LOCUS       DASTPM010000001         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_576630, whole genome shotgun sequence.
ACCESSION   DASTPM010000001 DASTPM010000000
VERSION     DASTPM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576630"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1905
                     /gene="secD"
                     /locus_tag="VFR42_00005"
     CDS             271..1905
                     /gene="secD"
                     /locus_tag="VFR42_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU0367559.1"
                     /translation="MNPNLKWKALFIVAVILVCIYGLIGLPTFPTSLAQIKDNFRKQI
                     KLGLDLQGGTHLVLQVQVQEAIAQETDQMVDRLTKQLRDKNISFDEIRRVDDTHILVR
                     NVDFQQAAAFRDLVQQQYGNLWDTAPAAGEQSGYTLTLKNSAIAAIQDSTMTQSLETI
                     DRRINALGLTEPTIQRRGRNENEILVQLPGEGDPEAAKRVIQAGGQLELRLVQDPRTY
                     SSQTEALSQHGGILPPGTELLQEDPRGRAASTQTPSGEVWYLVDRAPTVTGRDLRNAT
                     ENRSTNNPAQWQVNFVLSGEAARRFGPFTETNKGRQLAIVLEHKVYSAPVINGRIDDN
                     GMIEGNFGQESAHELALVLRAGALPASIKYLEQRTVGPSLGADSIRHGVQASVLSLVV
                     VMLFMLFYYRLSGANAVVALILNLIILLAALAYFGAVLTLPGIAGVILTIGMGVDSNV
                     LVFERIREELRNGKSAASAVSAGFDKAFLTIIDTHVTTVVSAFFLFLFGTGPIRGFAI
                     SLTIGLIANVFTAIYVSKTIFQYHLAKMDREAELSI"
     gene            2084..>2545
                     /locus_tag="VFR42_00010"
     CDS             2084..>2545
                     /locus_tag="VFR42_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367560.1"
                     /translation="MIEFFKEPNIDWMGKAKYFYALSAILLLAGWISIFAKGGMYYGI
                     DFKGGTNVDVKFAQPPNIDQLRGALSKQGLGNSEIQSIGDIGGNSNEVLIFVSQNGAN
                     DEALDSSKAQVLAALNATYNSSSGGKPDFNAATPSSLAAYLTQKDPLSLSSV"
BASE COUNT          563 a          697 c          751 g          534 t
ORIGIN      
        1 gaatggcgac aaagtggtga ccaccggcgg aatctatggc acggtcaatg gaatcgacgg
       61 cgacaccatc attctgaaaa tcgccgacca ggtgaaaatc cgcatcgcgc gttcggcgat
      121 tgcccaggtg gaggcgaccg aggatgccaa gtaacgcccg cctttgggca gcggaacgat
      181 ttttctctca gcggctgcac tgcgacactg ttgttctgaa ttcagcagcc gactctatct
      241 acggcggaga cgtagccgtc ctttctcatc atgaatccta atttgaagtg gaaagcgctg
      301 ttcatcgttg cggtaattct tgtctgcatt tatgggctga tcggattgcc gaccttccct
      361 acatcactgg cgcagatcaa ggacaatttc aggaaacaga tcaagctggg cttggacctg
      421 caggggggca cgcacctggt gttgcaggta caggtgcagg aagccatcgc gcaggaaacg
      481 gaccagatgg tggaccggct gacgaaacag ctgcgcgaca aaaacatcag cttcgacgag
      541 atccggcggg tggacgacac gcacattctg gtgcgcaatg tcgatttcca gcaagctgcg
      601 gcgtttcgcg atctggtgca gcagcagtac gggaatttgt gggataccgc tccggctgcg
      661 ggcgaacagt ctggttatac gctgaccttg aagaacagcg ccattgcggc gatacaagac
      721 agtacgatga cgcagtcgct ggaaaccatc gaccgccgca tcaacgctct gggcctgacc
      781 gagccgacga ttcagcgccg cggtcgcaat gagaacgaaa tcctggtgca gctgcccggc
      841 gagggcgacc cggaagccgc caagcgagtg attcaggccg gcggacaact cgaactacgg
      901 ctggtgcagg atccgcgcac gtacagctcg cagaccgaag cactcagcca gcacggcgga
      961 attttgccgc cgggcacgga gttgttgcag gaagatccgc gtggccgcgc cgcatcgacg
     1021 cagaccccca gcggggaagt ctggtatctg gtggaccgcg cgccgactgt gactggccgc
     1081 gacttgcgca acgcgacgga aaaccgcagc accaacaatc cggcgcagtg gcaggtgaat
     1141 ttcgtattgt ccggcgaagc agcgcgccgg tttggtccgt tcactgagac gaacaagggg
     1201 cgccagttgg caattgtgct ggagcacaaa gtctattccg ctccggtgat caacggccgg
     1261 atcgacgata acggcatgat cgaaggcaac ttcggtcaag aatcggctca cgaactggcg
     1321 ctggtgttgc gcgccggagc tttgccggct tcgatcaagt atctggaaca gcggacggtg
     1381 ggaccatcgc tcggcgccga ttcgattcgt cacggcgtgc aggcttcggt gttgagcctg
     1441 gttgtggtga tgctcttcat gttgttctat tacagactgt cgggagccaa cgcagtggtg
     1501 gcgttgatcc tcaacctcat catcctgctt gcggcattgg cctattttgg agcggtgctc
     1561 acattgcccg gaattgcggg tgttattctg accattggca tgggtgtgga ctccaacgtt
     1621 ctggtgtttg aacggatccg cgaagaactg cgcaacggaa agtcggcagc gtcggccgtt
     1681 tcggcgggat tcgacaaggc tttcttgacc atcatcgaca cgcacgtaac cacggtggtt
     1741 tcggcgttct tcctgttctt gtttggcacc gggccgattc gcggattcgc catttcgctg
     1801 accatcggtt tgatcgccaa cgtcttcacg gcgatttatg tgtccaagac gattttccag
     1861 tatcacctgg cgaaaatgga ccgcgaagcg gagctcagca tttagctgta ctaggactta
     1921 caagacaatc ttctcatttt ttatgggaag aacgctgtat tggttctgaa cccgttgtga
     1981 tggacgatgg cgggctgttg ctaccgtcca ttgggcagga accgacagcg agccgggcac
     2041 aaaccgcagc tcgctggagt ctaaggaagg gaagcaaatc ggaatgatcg agttcttcaa
     2101 agagcccaac atcgactgga tgggcaaagc caagtatttc tatgctttga gcgctattct
     2161 tttgctcgcc ggctggattt cgattttcgc caaaggcggt atgtactacg gaatcgactt
     2221 caagggcggc accaacgtag acgtaaaatt tgcacagccg cccaacattg accaactgcg
     2281 cggcgcgctc agcaaacagg gcctcggcaa cagcgaaatc caaagcattg gcgacatcgg
     2341 cggtaactcc aacgaagtgt tgatcttcgt gagccaaaac ggtgccaacg acgaagctct
     2401 cgactccagc aaggcacagg tactggccgc tctgaacgca acttataatt ccagctctgg
     2461 tggcaagccg gacttcaatg ccgccactcc ttcctcgctc gccgcttatc tgacgcaaaa
     2521 agacccgctg agtctttcct cggtg
//
LOCUS       DASTPM010000002         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_807274, whole genome shotgun sequence.
ACCESSION   DASTPM010000002 DASTPM010000000
VERSION     DASTPM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807274"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFR42_00015"
     CDS             <1..203
                     /locus_tag="VFR42_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEU0367561.1"
                     /translation="YVWNHLSSDFRPNNATTWNTEHGLEFNMLLDVAPMVLFQPVVQY
                     YANAGGGSEDAVVVGFRTKVEF"
     gene            237..1190
                     /locus_tag="VFR42_00020"
     CDS             237..1190
                     /locus_tag="VFR42_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU0367562.1"
                     /translation="MNIGGLISALLPVFFVLALGYFAGKRNAFDADQAAGLSKLALTF
                     ALPASLFVGMTDIRKELLLQQGPLVVALILSHAGLFLVAWLGLSFIKSLRGTASIIYA
                     LVLATSATPVFGLAVLQPILGPTSAGAVGLVALAINLTVPPAVVLLEMDAAARHQQAG
                     PGGSQPNPVLTGLNAGLRSPLLWAPILGIALVLAGIHLPPVVAACFQLIGSTTSGVAV
                     FAVGLVLAAHSIRLSPAVWVGSLGRITVQSAVLFALLHLMHVSSPFAREALVCCSFPL
                     ATVSVLFAARYKAVESEAASMLLVSTLALAITVPTILWLSK"
     gene            1328..>2588
                     /locus_tag="VFR42_00025"
     CDS             1328..>2588
                     /locus_tag="VFR42_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate decarboxylase family bicupin"
                     /protein_id="HEU0367563.1"
                     /translation="MNLNRTNSLNQKLNHITKQTKQQTIQLTNMSIQINDKKEQTITN
                     GSGEPIRGTWGAPILGPRNIAIERENPDLLASPETDAGTVPNLKFSYSAARNRLASGG
                     WAREVTVRELPVATKMAAVNMRLQPGGIREMHWHKEAEWGYVLAGRCRVTAVDAQGRN
                     FIDDVSPGDIWNFPAGIPHSIQGLEEGVEFLLVFDDGNFSENETFLISDWFAHTPREV
                     LAKNFGVSEAAFANLPKNVEKTRWIFPGKVPGPLASDAVVSPAGFVPESFTYRLSAQK
                     PIKVAGGQVRIVDSTKFPAASTIAAALVEVEPGGLRELHWHPNTDEWQYYISGKGRMT
                     VFADSGKARTFDYQAGDVGYVPFAMGHYVENTGDETLRFLEMFPSAYFADVSLNQWMA
                     LTPPELVQQHLNLDQQTLAVLRKDKPVI"
BASE COUNT          541 a          757 c          712 g          578 t
ORIGIN      
        1 gctatgtctg gaaccatctc agctccgact ttcggcctaa caatgccact acgtggaata
       61 ccgaacacgg ccttgagttc aacatgcttt tggatgtcgc gcccatggtc ctgttccaac
      121 cagtcgtaca gtattacgcc aacgcaggcg gagggtcaga ggacgcggtc gttgtcggat
      181 tcagaaccaa agtggagttc tagaaaatgc cttaagcgta tggaaggaag tgtctcatga
      241 acattggtgg attaataagc gcgctgctcc cggttttttt tgtcctcgcg ctcggctact
      301 ttgccggcaa acgcaacgcc tttgatgcgg atcaagccgc aggtttgagc aagcttgcgc
      361 tcaccttcgc gttaccggct tctctgtttg tcggcatgac tgatatccgc aaagagctcc
      421 ttcttcagca ggggccgctt gttgtagctc taattctttc tcatgccggc ctttttctgg
      481 tggcgtggtt aggcctcagc tttattaaat ctctgcgcgg gaccgcttct attatatacg
      541 cgctcgtgct ggcgacctct gccactcccg tttttggcct cgccgttctc caacctattc
      601 tcggtcctac cagtgcaggg gcggtcgggc ttgttgcgct cgcgatcaat cttacggtcc
      661 ctccggcggt cgtcctgctg gaaatggatg ccgcagcaag gcatcagcag gccgggcccg
      721 gcggttcgca gccgaacccc gtcttaacgg gcctgaatgc cggcctaaga tcacctttgc
      781 tttgggcgcc tatcctcggc attgctctcg tcctcgccgg catccatttg ccacccgtgg
      841 tagccgcctg tttccagttg attggctcga ccacttcggg cgtagcagtc tttgccgttg
      901 gtctcgtctt ggcggctcat tcgattcggc tttctcctgc cgtatgggtc ggttctcttg
      961 ggcgtatcac ggtgcaatcc gcagttttat tcgcgctgct gcatctgatg catgtcagca
     1021 gtccattcgc gcgcgaagca ttggtgtgct gcagttttcc acttgctact gtgagcgtgc
     1081 tatttgcagc acgctacaaa gccgtcgaat cagaagccgc ttcgatgctt ctcgtctcaa
     1141 cgcttgcctt ggcaatcacg gtgccgacca tcctctggtt gagcaaatga ttcacaaaaa
     1201 gaactccgac tggctactcc cagccgtttt gagtacgaaa gactcgccgg acacacaatt
     1261 acggctaacg atcgcacgcg agatgtcaga gttctgaaat ttcgttctcc gaaagcttga
     1321 gaaggaaatg aacctaaatc gaaccaactc actaaaccaa aaactaaatc acataactaa
     1381 acaaaccaaa caacaaacca tacaactaac caacatgagt atccaaatca atgataagaa
     1441 agaacaaacg ataactaacg gaagcgggga gccgattcgt ggaacctggg gcgcaccgat
     1501 cctggggcca cgaaacattg cgattgagcg cgagaaccca gatttactgg cctcgccgga
     1561 gaccgacgcc ggtaccgttc ctaacctcaa attctcctac tctgccgcca ggaaccgact
     1621 agcctcgggc ggctgggcgc gtgaggtaac agtgcgtgag ctgccggtcg ccaccaaaat
     1681 ggccgccgtg aacatgcgcc tccaacccgg aggtatccgc gagatgcact ggcacaagga
     1741 ggccgaatgg ggctacgtgc tcgccggccg ctgccgcgtc accgccgttg acgcccaagg
     1801 gcgcaacttc attgacgacg tcagcccggg cgacatctgg aacttcccgg ccggcattcc
     1861 ccactcgatc caagggctcg aggaaggggt cgagttcctg ctggtgtttg atgatggcaa
     1921 cttctcggag aacgagacct tcctcatcag cgactggttc gcgcacacgc cgcgtgaggt
     1981 gttggccaag aacttcggcg tgtccgaagc ggcctttgcc aatctgccta agaacgtgga
     2041 aaaaactcgg tggatctttc cgggcaaagt gcctggtccg ctggccagcg atgcagttgt
     2101 gtcacccgcg ggcttcgtgc ctgagagctt cacctaccgc ctctcggctc agaagccgat
     2161 caaagtggcg ggcggacagg tgcgcattgt cgattcgacc aaattcccgg ccgcgagcac
     2221 gatcgcagcg gcgctggtcg aggtcgaacc gggtggattg cgagagctgc actggcaccc
     2281 gaacacggac gagtggcagt actatattag tggcaaaggc cgcatgacgg tgttcgccga
     2341 cagtgggaaa gcgcggacct ttgactatca ggcgggcgac gtgggttacg tcccctttgc
     2401 aatgggtcac tacgtcgaga acacaggcga tgagacgctg cgcttcctgg agatgtttcc
     2461 cagcgcgtac tttgccgacg tgtcgctaaa ccagtggatg gcgcttacgc cacccgagct
     2521 tgtgcagcaa cacctgaact tggaccagca gacgctagcc gttctgcgca aggacaaacc
     2581 ggtcatcg
//
LOCUS       DASTPM010000003         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1326247, whole genome shotgun sequence.
ACCESSION   DASTPM010000003 DASTPM010000000
VERSION     DASTPM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326247"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFR42_00030"
     CDS             <1..292
                     /locus_tag="VFR42_00030"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU0367564.1"
                     /translation="LDPSSVAACRDADAILFFSPSAVHAFVALVDSGVLSPVADSTGI
                     GAIGPVTKENLLKEAKMRCDFEADEPSIEQVIAALTTFFAKGRATSAGAPVR"
     gene            289..1260
                     /gene="hemB"
                     /locus_tag="VFR42_00035"
     CDS             289..1260
                     /gene="hemB"
                     /locus_tag="VFR42_00035"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0367565.1"
                     /translation="MTFPVQRPRRLRKSELLRDLVRETRLSAKSFIYPLFVCPGSSVR
                     QEVGSMPGVFQQSADAIVEDCREVESLGIPGVILFGLPESKDPRGLSSLAQNGVVQRA
                     IDGIRKAKINLLVITDVCLCEYTDHGHCGVIENGEVANDPTLEILADQALSHARAGAD
                     IVAPSDMMDGRVGAIRTKLDANGFTDLPILSYAAKYCSGFYGPFREAADSAPQFGDRR
                     SYQMDPANAREALKEVALDLEEGADMVMVKPALPYLDIVRQVKDEFCVPVAAYNVSGE
                     YSMVKAAATNGWLDEKRVVFEILTGIQRAGADVILTYHAKDAARWLK"
     gene            1393..>2005
                     /locus_tag="VFR42_00040"
     CDS             1393..>2005
                     /locus_tag="VFR42_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0367566.1"
                     /translation="MSTSQPSPQHGRRRSREIFAEAEQILVGGVNSPVRAFRSVGGEP
                     LIIERGQGQYLFDADGNRLLDFVGSWGALLLGHAHPAVVAAIADQAQKGTSYGVTTEL
                     EVQLAQRIRRAVPFLEKIRFVSSGTEATMSALRLARGFTGRDVVIKFEGCYHGHADSF
                     LSQAGSGLATLGIAECPGVPQAVAQLTLNAPYNDLEAVEKLFSE"
BASE COUNT          393 a          670 c          523 g          419 t
ORIGIN      
        1 gctggatccc tcgagcgttg ccgcctgtcg agacgccgac gccattctct tttttagccc
       61 ttccgccgtg catgctttcg tggccttggt cgactccggc gtgctctccc cggtcgccga
      121 ctccaccggc atcggcgcca tcggacccgt taccaaggaa aatctgctca aggaagcgaa
      181 gatgcgctgc gacttcgagg ccgacgagcc ttccatcgaa caagtcatcg ccgcccttac
      241 cacgtttttt gcaaagggaa gagccacctc ggcgggggct cccgtacgat gacttttccg
      301 gtgcagcggc cgcgccgcct ccgcaaaagt gaacttctgc gcgacttagt ccgcgaaaca
      361 cgtcttagtg ccaagagttt tatctatccg ctttttgtgt gtcccggttc ttctgttcgc
      421 caggaagtcg gctccatgcc gggcgttttc cagcaatcgg ccgacgctat cgtcgaagac
      481 tgccgggaag tggaaagcct cggcatcccc ggcgttattc tctttggcct tcccgaaagt
      541 aaagatcctc gcggtctaag ctcactcgcg caaaacggcg tcgttcaacg tgccatcgac
      601 ggtattcgca aagccaagat caacctgctc gtcattacgg acgtctgcct ctgcgaatac
      661 accgaccacg gccactgcgg cgtcatcgaa aacggcgagg tcgccaatga tccaacactc
      721 gaaattcttg ccgaccaagc tctctcccac gcgcgtgcag gcgccgatat cgttgcgcct
      781 tccgacatga tggacggccg cgtcggcgcc atccgcacaa aacttgatgc gaacggcttc
      841 accgatctcc cgatcctctc ttatgccgca aaatactgct ccggcttcta tggccccttc
      901 cgtgaagccg ccgattccgc tccgcaattc ggcgatcgtc gcagctatca gatggaccca
      961 gccaatgccc gcgaagcttt gaaagaagtc gcgctcgacc tcgaagaagg cgccgacatg
     1021 gttatggtca agccggctct gccctatctc gacatcgttc gccaggtaaa agacgaattt
     1081 tgcgttcccg tagctgcgta caacgtcagc ggcgaatact cgatggtcaa agccgccgcc
     1141 acaaacggct ggctcgatga aaaacgcgtc gtcttcgaaa ttctcaccgg catccagcgc
     1201 gccggtgccg acgtcatttt gacgtatcat gccaaagacg ccgcccgctg gctcaagtag
     1261 caaccactgt tgaactcgtt tgccggcctt gcggtgactt acggggggcc atttggtctt
     1321 gtcgccaaaa gggactttaa ccctgaggaa ttgttttctg ctctagacgc aaattggaga
     1381 tttagacaac gtatgagcac cagccaaccc tcccctcaac acggccgccg ccgctcccgc
     1441 gaaatcttcg ccgaagccga gcaaattctg gtgggcggcg tcaacagtcc cgtccgtgct
     1501 ttccgctccg tcggtggaga acccctcatc atcgaacgcg gccagggcca atatctcttc
     1561 gatgccgacg gcaaccgcct tctcgatttc gtcggctcgt ggggcgctct actgctcggc
     1621 cacgctcatc ccgccgtcgt cgccgccatt gctgaccagg cgcagaaggg aaccagttac
     1681 ggcgtcacca cggaacttga agtccagctc gcgcagcgca tccggcgcgc cgtaccgttc
     1741 cttgaaaaaa tccgctttgt ttcgagcggc accgaagcca cgatgagcgc cctccgcttg
     1801 gcgcgcggtt tcaccggtcg cgacgtggtc atcaaattcg aaggatgcta ccacggccac
     1861 gccgacagct tcctatcgca agccggttct ggcctcgcca cgctcggcat cgcagaatgc
     1921 cccggcgttc cgcaggctgt cgcgcaactt accttgaacg caccctataa cgacctcgag
     1981 gccgtcgaga aacttttttc cgagc
//
LOCUS       DASTPM010000004         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_980302, whole genome shotgun sequence.
ACCESSION   DASTPM010000004 DASTPM010000000
VERSION     DASTPM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980302"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFR42_00045"
     CDS             complement(<1..690)
                     /locus_tag="VFR42_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0367567.1"
                     /translation="METIIEKDTIRTITTHFIDGAFVESHGREVMDIIKPSNGQLIGR
                     VTLGDEEDTRRAIAAAKRAFASFGRTTKEERSRYLRQLHKAVSARIDDLTAVMIEEYG
                     GVARFARLIVESGVNAFAAAESALQELPLRKSWGKTTVTLEPVGVAGLITAWNANALF
                     ICLKLASAVAAGCTVVLKPSEMSSLQTRVMVEALNAAELPKGVFNVVTGLGTVVGAEM
                     VRNPDVAKISFT"
     gene            complement(768..2162)
                     /locus_tag="VFR42_00050"
     CDS             complement(768..2162)
                     /locus_tag="VFR42_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0367568.1"
                     /translation="MRTWITLISAALFLSGCTMGPNYKRPTAAVPPAYRGIAADAPAQ
                     TESASLGDEKWWDVFQDEQLRSLVRTALQQNYDLRIAASRILEARAQLGITRADQFPA
                     VSGGAGITDVRTAQSKFLPAFETSTGQVNASAGWELDFWGKYRRATEAARANLLATEW
                     ARQEVASTLVANVAAAYFQLRALDLQLEISKRTLDSRQESLRLTRILANGGSTSLLDV
                     RQAEQLVFTASAEIPALEQQIEQQENFLSILLGRNPGNIARGQTLTEQHHPPEVPAGI
                     PSSLLERRPDIRQAEQQLVAANAEIGVARAAYFPQISLNGNGGFQSSALTNLFTGPAG
                     AWSFGASLTQPIFTAGKISSGVRLAEARQQTATLFYQQSVQGAFRSVSDALIGYRKTR
                     EFSSHQQELFESAQDAARLSHMRYNGGVTGYLEVLTNETNSFSAELGLAQARLNELLA
                     LVQLYEALGGGWQQ"
     gene            complement(2164..>3273)
                     /locus_tag="VFR42_00055"
     CDS             complement(2164..>3273)
                     /locus_tag="VFR42_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0367569.1"
                     /translation="NLQTFLTQARKRPELAGLSTTFLASVPQLYADVDREKLMKQGAS
                     VRDVYQTLQAFMGGYFINYFNRFGRQWQVYIEAEGDYRTQAENVSQFYVRNNKGGMLP
                     LSSVTRFESRDGPEFTMRFNEYRAAQINGAAAPGYSADQAMAALEDVFHKTMPREMGF
                     DYLGMSFQEKKAKEGISPAAIFGFSILFVFLILSALYESWSLPFSVLLSTPVAIFGAF
                     AVLWLRRTLLSTYLPSYMVQIESDVYSQIGLVMLIGLAAKNAILIVEFAKEQYEQGKP
                     LMDAALEGARLRLRPILMTSFAFILGAVPLWTATGAGAVARQIMGTAVIGGMLVASLV
                     GIFFIPAMFYLVEKWSGAGRKSAVISAHSAPAEAD"
BASE COUNT          696 a          977 c          905 g          695 t
ORIGIN      
        1 tgtgaacgat atcttcgcga cgtcgggatt gcgcaccatc tcagcgccga cgacagtacc
       61 gaggcccgtc accacgttga aaacaccctt gggtagctcg gcagcattca gagcctcaac
      121 catcacccgg gtctgcagcg aactcatttc gctcggctta agaacgacgg tacacccggc
      181 ggctacggcc gatgccagct tgaggcaaat gaacagcgca ttcgcgttcc aagctgtaat
      241 caaaccggca acgccgaccg gctccagggt gactgtcgtc ttgccccagc tttttcgcaa
      301 cggcagctct tgaagtgcgc tctccgccgc tgcgaaggcg ttgacgcccg actccacaat
      361 aagccgggca aacctggcga ctccgccata ctcctcaatc atgacagccg ttaaatcgtc
      421 gatacgagct gagaccgcct tatgtagctg gcgcaagtag cgcgagcgtt cttccttcgt
      481 cgttcggccg aaactggcga atgcgcgctt agccgcggca atagcgcgcc gcgtatcttc
      541 ctcgtctcct agcgtgaccc ggccgatcag ctgcccgttg ctcggcttga tgatatccat
      601 gacctcgcgg ccatgggatt caacgaacgc tccatcaatg aagtgcgtcg tgattgttct
      661 aatcgtgtct ttctctatga tggtttccat cgcgcttctc cttgcaaatg tatttccgtg
      721 ggaacgaagc aggctgccac cgcgctcctg taaggcgttt tcacgtctta ttgctgccac
      781 cctcctccca gtgcttcata cagctgaacg agagccaaca attcattgag tcgcgcctgt
      841 gcgagaccca attccgccga gaaggaattc gtttcgttcg tcaagacttc gagatagccc
      901 gtgacgccgc cgttgtagcg catgtgagaa aggcgtgcag cgtcctgtgc ggattcgaag
      961 agctcctgct ggtggctgct aaattcccgc gtcttgcgat aaccgatcaa ggcgtcggac
     1021 acgctgcgaa aagcaccctg aacggattgt tgataaaaga gcgtggccgt ttgctggcgg
     1081 gcttcggcta gacgaactcc agagctaatc tttcccgccg taaagatagg ctgcgtcagc
     1141 gaagcgccaa aactccacgc gccggcgggt ccagtaaaca gattcgtcag cgccgaactc
     1201 tgaaagccgc cattcccgtt aagcgagatc tgaggaaaat aagcggcacg cgcaacgccg
     1261 atctccgcat tcgcggcgac cagctgttgt tccgcctgcc gaatgtcggg ccggcgctca
     1321 agcaaagatg acggtatacc agcgggtact tccggagggt ggtgctgctc agtgagcgtt
     1381 tgacctcgcg ctatgttgcc gggattccgg ccaagcaaaa tgctgagaaa gttctcttgc
     1441 tgttcaattt gctgttcgag ggcaggaatt tccgcgctgg ccgtgaagac caattgctcc
     1501 gcctggcgca catcgaggag cgaagtcgaa cccccgtttg ccaagattct tgtcagtcgc
     1561 agagactcct gccgtgaatc gagcgtacgt ttcgaaatct ccagctgtag atcaagcgct
     1621 cgcagttgga agtaagctgc ggcgacgttc gccaccagcg tcgaagccac ttcttgcctc
     1681 gcccactccg tcgcaagcag attggcgcga gcggcttccg tcgcgcgacg atactttccc
     1741 cagaagtcca gttcccagcc agccgaggca tttacctgtc ccgtgcttgt ttcaaaggct
     1801 ggaaggaatt tcgattgggc cgtgcgaaca tccgtgattc ccgccccgcc gcttaccgct
     1861 ggaaactgat cggcgcgcgt aatcccaagt tgtgctctcg cttcaaggat gcgggacgcg
     1921 gcgatgcgca aatcgtagtt ttgctgtagt gccgtgcgga ccagcgatcg cagctgttcg
     1981 tcttggaaaa cgtcccacca cttctcatct cccagagaag cactctccgt ttgtgctggc
     2041 gcgtcagcgg ctatgccgcg gtacgcagga ggaacggcag ccgtcggccg tttgtagttt
     2101 ggccccatgg tgcagccgga aaggaaaagg gcggcagaaa tcagcgtgat ccacgttctc
     2161 atgctaatcc gcctcggccg gagcgctgtg ggcggaaatt accgcgctct tccggcccgc
     2221 gccagaccat ttctcgacaa gatagaacat ggcggggatg aagaagatgc cgacgaggct
     2281 cgctaccagc attccgccta tgaccgcggt gcccataatc tgccgagcca cggctccggc
     2341 cccggtagcg gtccacagag ggaccgcacc caaaatgaac gcgaatgaag tcatcaggat
     2401 cgggcgaagc cgcaggcgcg cgccttcgag cgccgcatcc atgagtggct tgccttgctc
     2461 atattgctct ttcgcaaact caacaatgag gatcgcgttc ttcgcagcga gaccaatcaa
     2521 catgaccaga ccgatttgcg aatacacgtc gctttcgatc tgcaccatgt aactcggcaa
     2581 gtatgtcgac agcagcgtgc gcctcaacca gagaactgca aacgctccga agatcgctac
     2641 gggtgtgctg aggagtacgc tgaatggcag cgaccagctt tcatagagcg ccgaaagaat
     2701 cagaaagacg aacagtatcg aaaatccgaa aatggccgct ggcgaaatac cttccttggc
     2761 tttcttttcc tggaaggaca tgccgagata gtcgaagccc atttcacgcg gcatggtctt
     2821 gtgaaataca tcttcgagcg ccgccatggc ctggtccgcg ctgtaacccg gagctgccgc
     2881 gccgttgatt tgggcagcgc gatattcgtt aaagcgcata gtgaattcgg gtccatcgcg
     2941 ggactcgaaa cgcgttacag acgaaagagg aagcattccg cccttgttgt ttcgcacgta
     3001 aaactggctg acattttcgg cttgcgtgcg ataatcgccc tccgcctcga tgtacacctg
     3061 ccactgtctt ccgaaccggt tgaagtaatt aatgaagtag ccgcccatga acgcctgcag
     3121 cgtttggtag acatccctga cggatgcacc ttgcttcatc aacttttcgc gatcgacatc
     3181 cgcgtaaagc tggggcacgc tcgcgagaaa cgtcgtgctc agccccgcga gttccggccg
     3241 tttccgggcc tgtgtgagaa acgtctgcag gtt
//
LOCUS       DASTPM010000005         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_490255, whole genome shotgun sequence.
ACCESSION   DASTPM010000005 DASTPM010000000
VERSION     DASTPM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490255"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..2442
                     /locus_tag="VFR42_00060"
     CDS             364..2442
                     /locus_tag="VFR42_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU0367570.1"
                     /translation="MFVVEWLAKLFFANPLLIGLTAVAVWSFVSSLRRIGPTEVGLVT
                     KRFSFKKLAKDNPVAFSGEAGYQADLLMPGLRWKPFFLYAVEKYPWVQVPAGEIGVVI
                     AQVGNPLPIGAKSAVYKKEFANFSDLRSFVQLGGQKGVQRPVLPPGSLVPIHPLGFLV
                     LTRSRVYGVPVSPDVRAKMGRGGELSPVALGLRPESLELVRIEPQPRGKDGTTLDFVG
                     IVTTYEGDPLPSGDIASRLDGYADIAKIEAAENKASDAQIIETLLGSKNQLHNNYQDF
                     QAFLDHGGHIGLQHDPLQYGAYALNPFLVSVELVPMMVVKQGQVAVVKAYVGLATEDT
                     SGAEFKYGSLVRPGHRGVWQEPLRTGKYPLNPRCYESEIVPTAILNLNWADAVSEAHN
                     LDAQLQQIVAKSKEGFVFKIDLQVQIHVSDTKAPRVISMVGTMKNLVNEVLQAAVGNH
                     FRNSLQSMPAISFIETRQQVQIEAFQHICQQLEQYQVETKGVYIQDVVLPEDMVTVLT
                     HREVANQEIETFKKQQAAQQQRIEMEQAKGTADMQADLARSKVGVDIKKNNADARVAE
                     ATGEAEYIRQTGTAKGAEVEAIGLARARGYRAQVEALGSNATAIVNVVAALAEGKAKF
                     VPDVLVAGGGNGNGAIEGLAATAMRFFGSGNGGSSASTAAGKLPTAPLTPEGEAAMKK
                     LMPKETPAKS"
BASE COUNT          600 a          651 c          899 g          498 t
ORIGIN      
        1 tgtgcccggt ctgtctgaaa gcccggaatt catttcttca ggggctgaag cccggagact
       61 aattgccaag caattatgtc ggagctaagg ctccgacccc cggttgccca cagactcatg
      121 tccccacaag cggacctgtg atggatctat ggtagcgcgt tgagcggttt cggcgggaaa
      181 cctcggaacg gcgtatgtcc attgcgtaaa gtcgtatctc actgaaaata agccagttta
      241 agttacgtac tgtgctctac ttgacaggcg cgttgcagac gcgcagattc ttgcccgtcg
      301 gaacatgtaa cccccttaga caaggcaagt tgaggtagcg cctggagcgc cggaggtgtg
      361 tgtatgttcg ttgttgagtg gcttgcgaag ctgttttttg ccaacccgct gctgattgga
      421 ttgactgcgg tggcggtgtg gtcgtttgta tcgtctttgc gccggatcgg gccgacggaa
      481 gtcggactgg tgacgaagcg gttttcgttc aagaagctgg cgaaggacaa cccggtggcg
      541 ttcagcgggg aggccggtta tcaggccgac ttgttgatgc ccgggctgcg ctggaagccg
      601 ttttttctgt acgcggtgga gaaatatcct tgggtgcagg tgccggcggg cgaaatcgga
      661 gtggtgattg cgcaggtggg caatccgctg ccgatcggcg cgaagtcggc ggtgtacaaa
      721 aaggagttcg cgaacttttc cgatctgcga tcgtttgtgc agctgggcgg acagaaaggc
      781 gtgcagcggc cggtgttgcc tcctggatcg ctggtgccga ttcatccgct gggatttttg
      841 gttctgacaa ggagccgggt gtacggagtg cccgtatcgc cggacgtgcg ggccaagatg
      901 gggcgaggcg gcgagctgag tccggtggct ttgggactgc ggccagagag cctggaactt
      961 gtgcgcatcg agccgcaacc gcgcggcaag gatggaacga ctttggactt cgtgggaatt
     1021 gtgacgacct acgaaggcga cccgctgccc tcgggcgaca ttgctagccg tctggatggc
     1081 tacgcggaca tcgcgaagat cgaagccgcg gagaataagg cgagcgatgc gcaaatcatc
     1141 gagacgttgc tgggcagcaa gaaccagctg cacaacaact atcaggactt ccaggcgttt
     1201 ctggatcacg gcgggcacat aggattgcag catgacccgc tgcaatacgg ggcgtacgcg
     1261 ctgaatccat ttttggtgag cgtggagctg gtaccgatga tggtggtgaa gcagggccag
     1321 gtggcggtgg tcaaggcgta cgttggactg gcgacggaag atacgtccgg cgcggagttc
     1381 aaatacggct cgctggtacg gccgggacat cgtggcgtgt ggcaggagcc gctacgcacg
     1441 ggcaagtatc cgctgaaccc gcggtgttat gagtcggaaa tcgtgccgac ggcgattctc
     1501 aacttgaatt gggcggacgc ggtgagcgaa gcgcacaacc tggacgccca actgcagcag
     1561 atcgtggcga agtccaaaga aggcttcgtt ttcaagattg acttgcaggt gcagatccac
     1621 gtgtcagaca cgaaagcgcc gcgcgtgatt tcgatggtcg gcacgatgaa gaacctggtg
     1681 aatgaagtgc tgcaggcggc ggtgggcaac cacttccgca actcgctcca gagtatgccg
     1741 gcgatcagtt ttattgagac gcggcagcag gtgcagatcg aagcgtttca acacatttgc
     1801 cagcagctag agcaatacca ggtcgagacg aaaggcgttt atatacagga cgtggtgctg
     1861 ccggaagaca tggtgacggt gctgacgcat cgcgaggtgg caaaccagga aatcgagacg
     1921 ttcaagaaac agcaagcggc gcaacaacag cgcatcgaga tggaacaagc caagggtacg
     1981 gccgatatgc aggcggacct ggcgcggtcg aaggtgggag tggacatcaa gaagaacaac
     2041 gcagatgcgc gtgttgcaga agcgaccggc gaagcggaat acatccggca gaccggtacg
     2101 gcgaagggcg cggaagtgga agcgatcgga ctggcgcggg ctcgcggtta tcgcgcgcag
     2161 gtggaagcgt tgggttccaa cgcgacagcg atcgtcaacg tggtggcggc gctggcggaa
     2221 gggaaggcga agttcgtgcc ggatgtgctg gtggccggag gaggaaacgg caacggcgcg
     2281 atcgaagggc tggcggctac agcgatgcgg ttctttgggt cgggtaatgg aggatcgagt
     2341 gccagtaccg cagcgggaaa attgccgacc gcaccgctga ctcccgaagg cgaagcggcg
     2401 atgaagaagc tgatgcctaa ggagacgccg gccaagtcct gaaggcaggg aaaatagcta
     2461 aacacgaaaa tcgaaatact aaactcgaaa aacgaattac ggaggatcac acccagaagg
     2521 tgaaaaagcg aaactcgaaa aacagacgtt agaaaacaga agcggctccc tacccagcca
     2581 cagggagccg ctatctgtac cagagaacct ccgctgcctc tcccttattc ctctattggt
     2641 tccctggg
//
LOCUS       DASTPM010000006         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_922753, whole genome shotgun sequence.
ACCESSION   DASTPM010000006 DASTPM010000000
VERSION     DASTPM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922753"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFR42_00065"
     CDS             <1..509
                     /locus_tag="VFR42_00065"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367571.1"
                     /translation="DLISPVVERAQRSCVDATGIGAQLAEDLRAKHGSKVEEVVFNLE
                     NKEKMATLTKTMFEERKCRIPSSPILRRSINAVKRYTSPTGHFRFDAERTEAGHADEF
                     WALALAIAAGASARPALGVAVDSRKDWYTNQQRGIMTRQAQEISGRDREQQQPAVTGG
                     RRSLWASR"
     gene            494..1984
                     /locus_tag="VFR42_00070"
     CDS             494..1984
                     /locus_tag="VFR42_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367572.1"
                     /translation="MGFSLVKTVQGWGSTVRKVFGKKVAEAVQPFLFGLTAADGEDAK
                     FRRITSPNSIRDLNPLMHERMQAVCFYLKSTTPFGKRIVEVITSYVVGEGFKPAATDP
                     DVQEVVTKFWNDQVNNLDQALKDYCDELTTFGELCLPVAVNPVDGAVRLGYIDPLMLD
                     SVEYGSIETASGQQQIMVPVAVWLKKKQMETVGQRLEIVRVDEDPNSETFGQLKGDCF
                     YFCINKAKGASRGLSELFSLADWIDVFDQMMFDFADRIRFLNAFVWQYTIKGAGEPEL
                     EKWNKKVTQNPPRQGSVQVTNENVEIKAQTPSFAGADMTQATRSIKLYGLGGAGLPAW
                     FFADPVDSNKSTADEMTGPTGKKLTDRQNNLKSAIMQVLTFVVQQAIYHGVLPEDIDT
                     TLSLQVPDLMIKDLQKAAQTLAAVVNSVSIMAENGYIQQKTAARASHIVLTQIGIEVD
                     PDEFDKAQAEKNTRDAELVPEQTVLSKALTQLETGKVPGAGAGAVQ"
     gene            1981..>2310
                     /locus_tag="VFR42_00075"
     CDS             1981..>2310
                     /locus_tag="VFR42_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367573.1"
                     /translation="MSVQTQFSAKIADLIAQSANLSLEARGKVLEMLDAVRTEITARL
                     ANLNAASFTAAQLTVLKGQIDALFKKFSVDATRLIDSYEAQEFNLGSQMIVQPISILG
                     TPTLGQIS"
BASE COUNT          532 a          648 c          716 g          414 t
ORIGIN      
        1 tcgatctgat cagcccggtc gttgagcggg cgcagcgctc atgtgtagac gcgacgggca
       61 tcggcgcgca gctggcagaa gatctccgcg caaaacatgg cagcaaggtt gaagaggtcg
      121 tattcaatct ggaaaacaaa gagaagatgg cgacgctcac gaagacgatg ttcgaggagc
      181 ggaagtgccg cattccctca tcgccaatac tgcggcgctc catcaatgcg gtgaagcgct
      241 atacctctcc aacgggccat ttccgttttg atgctgaaag aacggaagct gggcacgctg
      301 atgaattctg ggcgctggct cttgcaattg cagccggcgc gagcgcacgg ccagcgcttg
      361 gcgtcgcggt cgattctcgg aaggattggt acaccaacca gcagcgcggc atcatgacgc
      421 gccaggcgca ggagatctcc gggcgcgatc gcgaacagca acagccggca gtgactggcg
      481 gaaggaggtc cttatgggct tctcgctagt caagacggtg cagggctggg gcagcacggt
      541 gcgcaaagtc ttcggcaaga aagtggctga agccgtgcag ccgtttctct tcggcctgac
      601 ggccgctgat ggcgaggacg cgaagttccg gcgcatcact tcgcccaaca gcatccgcga
      661 tctcaatccg ctgatgcatg agcgcatgca ggccgtgtgc ttctatctga agtcaacgac
      721 gccattcggc aagcgcattg ttgaagtcat caccagctac gtggtgggtg aaggattcaa
      781 gccagccgcg accgatcctg acgttcagga agtggtgacg aagttctgga atgaccaggt
      841 caataacttg gaccaggcgc taaaggatta ttgcgacgag ctcaccacgt ttggagagct
      901 ctgtcttcct gttgcggtga atcccgtgga cggcgcggtt cggctgggat atatcgatcc
      961 gctgatgctc gattcggtgg agtacggcag catcgagacg gccagcggcc agcagcagat
     1021 catggttccc gtcgcggtgt ggctgaagaa aaagcagatg gaaactgtcg gacagcggct
     1081 ggagatcgtg cgcgtcgacg aggacccgaa ctccgagacg ttcggccagc tcaaaggcga
     1141 ctgcttttat ttctgcatta acaaggccaa gggcgcaagc cgcggactct ctgaattgtt
     1201 cagcctggcg gactggatcg atgtcttcga ccagatgatg tttgacttcg ctgatcgcat
     1261 tcgcttcctt aacgcgttcg tgtggcagta cacgatcaaa ggcgcgggcg agcctgagtt
     1321 ggagaagtgg aataagaaag tcacgcagaa cccgccgcgc cagggcagcg tccaggtcac
     1381 caatgaaaac gtggagatca aagcgcagac gcccagcttc gcgggcgcgg acatgacgca
     1441 ggccactcgc tccatcaaac tctatggcct gggcggagct ggactgccgg catggttctt
     1501 cgccgatccc gtcgacagca acaaatccac ggccgacgaa atgaccgggc caaccggaaa
     1561 gaagctgacg gaccgccaga acaatttgaa gagcgcgatc atgcaggtcc tcaccttcgt
     1621 ggtgcagcag gcgatttatc acggagtgct tccagaagac atcgacacga cgttgagcct
     1681 gcaagttccg gacctgatga tcaaggacct gcagaaggcc gcgcagacgc tggctgctgt
     1741 tgtgaactcc gtctccatca tggccgagaa cggttacatc cagcagaaga cggccgcgcg
     1801 cgcgtcgcac atcgttctca cgcagatcgg cattgaagtt gacccggatg agttcgacaa
     1861 agcgcaggcg gaaaagaaca cgcgcgatgc ggagctggtc cccgagcaga cggtcctgag
     1921 caaagcgctc acgcaacttg aaactgggaa agtgcctgga gccggagcag gagcggtgca
     1981 atgagcgtgc agacacagtt ctcggccaag atcgcggacc tgatcgcgca gtcggccaac
     2041 ctctcactcg aagcgcgcgg gaaagttctg gagatgctgg acgcggtccg cacagaaatc
     2101 accgcgcgcc tggcgaacct gaatgccgcc agcttcaccg cagcgcaact caccgtgctc
     2161 aaaggccaga tcgacgcgct tttcaagaaa ttcagcgtgg acgcaaccag gttgattgac
     2221 agctacgagg cgcaggaatt caatctcggg tcgcagatga tcgtgcagcc gatatccatc
     2281 ctgggcactc cgacgctggg ccagatatcc
//
LOCUS       DASTPM010000007         5522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1066986, whole genome shotgun sequence.
ACCESSION   DASTPM010000007 DASTPM010000000
VERSION     DASTPM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066986"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFR42_00080"
     CDS             <1..660
                     /locus_tag="VFR42_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0367574.1"
                     /translation="RGLVTATIGHLQIFRERDFWGCAAGHFSFNYVSYFFLTWLPFYL
                     SHERHLSQQALARAAGAYYLLDSASALSTGWLCDRWIQRGANAVFVRKAASAGGHVLA
                     AVAMIACAFAGADSYYPWLLLAAVGSGAAACGVFLFAQTIAGPRAVGQWSALQNGFGN
                     FAGLTGPALTGFLVDRSGHFFSAFALAAGISLAGALVWAFGVRLAPIQWPEPRAPQPG
                     E"
     gene            740..922
                     /locus_tag="VFR42_00085"
     CDS             740..922
                     /locus_tag="VFR42_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367575.1"
                     /translation="MPYGPVLCVQCDKPEYDCTCEKYCTICKSLDNVHLCTDGLYYCP
                     DCREACDVALAESRER"
     gene            912..1205
                     /locus_tag="VFR42_00090"
     CDS             912..1205
                     /locus_tag="VFR42_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367576.1"
                     /translation="MNAEPKRILLRMDSDPRFAAAAGGAVRFLAEAAGMPEDVCKEFQ
                     EATVRACSKAFDAQPKQPHTVEFLVFGDRLEVAIDADAGSHAIRLSRSVVPQR"
     gene            complement(1289..2320)
                     /locus_tag="VFR42_00095"
     CDS             complement(1289..2320)
                     /locus_tag="VFR42_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367577.1"
                     /translation="MRKTVLRIAAAAAVTMASLVTAQVSSAQRVGPDTIALFPKNVGE
                     FAYANLKQARSQKWYAQLQQQLLPERFRQFEKFLASAGVDPNTQVEELAWGLVAEGVN
                     DKTSGVGSDAVPTGEEVAGIAMGNYNPTSTEAFFKAQKLPTFTSHGFTMYAFGSGSGA
                     SDLFFLFIDSNTAAFGHRNVLEKMLDVRYGGAEGLLRNDKMYSLINEANGSGVVWAVL
                     NPAYTRLAVRQLAPQIQQFPEAAKLVTRMQNLILNIQASGGVDGKFQAVCGNTDDANT
                     LAQLMQLALVYQQYQAKQQGQTQNQDLADLLSQAQVSPSGDRVVLRLSLTDDQMTSLI
                     KKNTFALKM"
     gene            complement(2358..3887)
                     /gene="ftcD"
                     /locus_tag="VFR42_00100"
     CDS             complement(2358..3887)
                     /gene="ftcD"
                     /locus_tag="VFR42_00100"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEU0367578.1"
                     /translation="MKRLIECVPNFSEGRDPEKVDALIQAMSKVPGVWVLDYEMDADH
                     NRCVITLAGEPDAVAEAALQGTGKAVELIDMTQHTGAHPRVGATDVVPFIPVEGVSLE
                     DCVALARRVGQEIWKRYRIPVFFYEAAATRPDRVNLENIRRGQFEGLREELKKNHDRQ
                     PDVGEPKLHPTAGVTVVGARKFLVAYNVNLNTSDVGIANKIAKAIRFSSGGLRYVKSM
                     GVDLKARHIAQVSINLTDYEQTPMHRVYEMVKREAARYGAVPVGSEIVGLVPKKAIEM
                     AADYFLQLESFSPSQVFENKLADALVGAPLEEKKDGKLAALARPLVDAVAEATATPGG
                     GSVSAFAAALAAALGQMVAGLSRKKKSQAAHVDKLSEHLDALRREAEALTQAIDADAA
                     SYDAVMAAFKLPQGGSTEIAQREEAIQKSTKGAAEVPLQVAERAVALLERLRQLETIA
                     AASMKSDLRVARLVAAAGARGAIANVEINLDGIKDQGYVAAKRQAISVLIDKLGSVPA
                     F"
     gene            complement(3981..5246)
                     /gene="hutI"
                     /locus_tag="VFR42_00105"
     CDS             complement(3981..5246)
                     /gene="hutI"
                     /locus_tag="VFR42_00105"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU0367579.1"
                     /translation="MSNSLLIRGASQIVTVSGRAPRRGKALADIGVLRDGAVLLRDGV
                     ISAVGPAAKIDKLKESKSAEVLDLDGRVVLPGFVDSHTHLIHAASRAEEYELKIQGAG
                     YEEIAKKGGGILDSVGKLRVETAQELKSRARMFLERFAEHGTTTIEAKSGYGLEVTSE
                     LRILSLHKELDAEQPLEIVSTFLGAHVVPAEYRNKASGRRDYLRLLEQELIPEVAENG
                     LAEYGDVFCERSAFSRAESRQVLLAGKKHGLRPRVHAEQLSNTGATRLGIELGAASCD
                     HLEHLKPADIRALAKSNTAATLLPGCDFHLGLKQYAPARKLINEGSIVALATDFNPGT
                     SPTLSMPMVLSLACTQLRMTPAEAITAATLNAAYSLGRHRRIGSIEAGKQADLAVFEV
                     ADYREIPYYFGVNTCWLTMKRGEVIHMRD"
     gene            complement(5282..>5522)
                     /locus_tag="VFR42_00110"
     CDS             complement(5282..>5522)
                     /locus_tag="VFR42_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367580.1"
                     /translation="GKPKLSYQKFPAFLVEQGIKQLGIPKKTASLMTEMHEAGNEGLL
                     NPQEPRSEQNTTPTSIEAFAQEEFAPAYQAKAASA"
BASE COUNT         1005 a         1596 c         1682 g         1239 t
ORIGIN      
        1 cgaggactgg tgaccgcaac gatcggacac ctgcagattt ttcgcgaacg tgatttttgg
       61 ggttgtgccg ccgggcactt ttcctttaac tatgtttcct acttttttct gacctggctg
      121 ccattttatc tttcgcacga acgccatctt tcgcagcagg ctttggcgcg cgcagcggga
      181 gcatactacc tgctggattc cgcatcggca ctgtcgaccg gctggttatg cgaccgctgg
      241 atacaacgcg gggccaacgc agttttcgtg cgcaaagctg cttcggcggg aggccatgta
      301 ctagcggcgg tggccatgat cgcgtgtgct tttgcaggcg cggactccta ttacccctgg
      361 ctgctgctgg cggctgtggg ctccggggcc gccgcctgcg gcgtctttct gttcgcgcag
      421 acgattgccg gaccgcgcgc ggtggggcag tggtccgcgc tgcaaaatgg ctttggcaac
      481 tttgccggac ttaccgggcc cgcgttaacg gggtttctgg tcgaccgcag cgggcacttt
      541 ttttcggcgt tcgcgctagc ggcaggaatt tccctggctg gcgcactggt atgggcattc
      601 ggagtacgcc tggcgccgat ccagtggcca gagcctcgtg ctccccaacc gggcgaatga
      661 cagaaagcct cataacaaag agcaagcgct ccttgccgcg acgctttgcg gtcaggtaaa
      721 gtttcttctg gagattgcga tgccttacgg ccccgtcctg tgtgtgcaat gcgacaagcc
      781 ggagtatgac tgtacgtgcg agaaatactg caccatctgc aagagcctgg ataacgtgca
      841 cttgtgcacc gacggtttgt attattgccc ggactgccgg gaagcctgcg acgtagccct
      901 cgcggagtca cgtgaacgct gagccgaaac ggattttgct gcgtatggac tccgatccgc
      961 gcttcgccgc ggctgccggc ggcgcggtgc gattcctggc ggaggccgcg ggaatgcccg
     1021 aggacgtctg caaagagttt caggaagcta cggtgcgcgc gtgtagcaag gcgtttgacg
     1081 cgcagcccaa acagccgcac accgtggagt ttctggtgtt tggcgaccgg ctggaagtgg
     1141 caatcgatgc ggacgccggt tcgcacgcga ttcgcttgag ccgctctgtg gttccccagc
     1201 gttagagaaa aatcgtccga aaacaaaata ggagtggacg tgcgccggta atcccgcctg
     1261 atcgaactac ccgtcgagtt tcaaggagct acattttcag cgcgaacgtg tttttcttga
     1321 tcaacgaagt catctggtcg tcggtcagcg agagccgcaa gaccacgcga tcccccgacg
     1381 ggctcacttg ggcttggctc agaaggtcgg ccaggtcctg gttctgggtt tggccctgct
     1441 gcttggcctg atactgctga taaacgagcg caagctgcat tagttgagcg agcgtgttgg
     1501 cgtcgtctgt atttccacaa accgcctgaa attttccatc cacgccgccg ctggcttgaa
     1561 tgttcagaat caagttctgc atgcgcgtga ccagcttcgc cgcttcgggg aattgctgaa
     1621 tctgcggagc gagctggcgc accgccaggc gcgtgtaagc tggattcaac acggcccaca
     1681 ccacaccgct accgttggct tcgttgatca gcgaatacat cttgtcgttg cgcagcaaac
     1741 cttccgcgcc gccgtagcgg acatccagca tcttttcgag tacgttgcga tgaccgaagg
     1801 ctgccgtgtt ggaatcgatg aacaggaaaa acagatcgct cgccccggag ccgctgccaa
     1861 aggcgtacat ggtgaagccg tgcgaggtga acgtcgggag cttttgcgct ttgaaaaagg
     1921 cttcggtgga agtgggattg tagttgccca tggcgatgcc ggcgacttct tctccggtgg
     1981 gcacggcgtc tgagcctacg ccggaagtct tgtcgttcac gccttcggcc accaggcccc
     2041 acgccagttc ttcgacctgc gtgttcgggt ccactcccgc cgaggccaga aatttttcga
     2101 actggcggaa ccgctctggc aaaagctgct gttgcagctg tgcataccat ttttgcgaac
     2161 gcgcctgctt gagattggcg taggcaaact cgcccacgtt tttcgggaat aatgcgatgg
     2221 tgtccgggcc tacgcgctgt gcgctggaaa cctgcgccgt tactagcgac gccatcgtga
     2281 cggcggccgc ggccgcaatc cgcaaaacgg tcttcctcat ttcgtgctcc ttgctgattc
     2341 tcggcggtgc tgagctgcta aaacgcaggc accgaaccta atttgtcgat cagaacgctg
     2401 atggcctgtc gtttcgccgc cacgtacccc tgatctttga tgccgtcgag attgatctcc
     2461 acgttggcga tcgctccgcg cgcacccgct gcagccacca atctggccac ccttaagtct
     2521 gacttcatgg atgccgcggc gatagtctcc agttgtctta gacgttcgag gagtgcaact
     2581 gcccgttcgg ccacttgcaa agggacttcg gcggcgccct tggttgactt ttgtatggct
     2641 tcttcgcgct gcgcgatctc ggttgagccg ccctgcggca gtttgaacgc cgccattacg
     2701 gcgtcgtagg acgcggcatc cgcgtcgatg gcttgggtga gagcttcggc ttcgcggcgc
     2761 agggcgtcaa gatgctcgga gagtttgtcc acgtgcgcgg cttgcgattt ctttttgcgg
     2821 gaaaggccgg caaccatttg gccgagagcg gccgcgagtg ccgctgcgaa tgctgaaacg
     2881 ctgccgccgc cgggagttgc tgttgcctcg gcaacggcat ccaccaacgg ccgagccagc
     2941 gcagcaagtt tgccgtcctt tttttcttcg agtggcgcgc ccaccagggc atcggccaat
     3001 ttgttttcaa aaacctgcga cggagaaaag ctttccagtt gcaggaaata gtcggcggcc
     3061 atttcgatgg cttttttcgg gaccagaccg acaatttcgc tgcctacggg aaccgcgcca
     3121 tagcgggcgg cttcgcgttt caccatctcg tagacccgat gcatgggagt ttgctcgtaa
     3181 tcggtcaaat tgatggaaac ctgggcgatg tggcgcgcct ttaagtcgac gcccatcgat
     3241 ttcacgtaac gcaggccgcc gctcgaaaag cggatggctt tggcgatctt gttggcgatg
     3301 ccgacgtcgg aagtgttcaa gttcacgttg taggcgacca aaaacttgcg cgcgcccaca
     3361 accgtaacgc cagccgtagg atggagtttg ggctcgccga catcgggctg gcgatcgtgg
     3421 tttttcttca gctcttcgcg caagccttcg aactggccgc ggcgaatgtt ttccaggttg
     3481 acgcgatccg ggcgcgtggc ggccgcttca tagaaaaaga ccggtatgcg gtagcgtttc
     3541 cagatttcct ggccgactcg gcgggccagg gcaacgcaat cctcgagtga aacaccttca
     3601 accggaatga acgggacaac gtccgtggcg ccgacccggg gatgcgcacc ggtgtgctgg
     3661 gtcatatcaa tcagctcaac ggccttgccc gtgccttgca gcgcggcttc ggcaacggcg
     3721 tcgggttcgc cagccagcgt aatgacgcag cggttgtgat cggcgtccat ctcgtagtcc
     3781 aacacccaga cacccggcac cttgctcatg gcttgaatca gtgcgtcaac tttttcgggg
     3841 tcgcggcctt cgctgaaatt cggcacacac tcaatcagtc gtttcatgga aaagtcaccg
     3901 ccgccttaag tgaattctgg ttgagcggct acctcggagt agccgacctg gttcttgagc
     3961 cgcagttctg accgaatgcc tcaatctcgc atgtggatga cttcgccgcg cttcatggtg
     4021 agccagcaag tgttcacacc aaaatagtag ggaatttcgc gatagtccgc cacttcgaag
     4081 accgcgaggt cggcttgctt gccagcctcg atcgagccga tgcgcctgtg gcgaccaaga
     4141 gagtacgcgg cgttcagagt cgcagcggtt atggcctcgg cgggcgtcat gcgcagttgc
     4201 gtgcaggcca gggaaagcac catgggcata ctgagcgtag gactggtgcc cgggttgaaa
     4261 tccgtcgcca gcgcaacgat tgagccttcg ttgatcagtt tgcgcgcggg agcgtattgc
     4321 ttcaggccca gatggaagtc gcagccgggt agcagtgtgg cggcggtgtt ggattttgcc
     4381 agcgcgcgaa tgtcggcggg tttcagatgt tcgaggtgat cgcagctcgc tgcgccgagc
     4441 tcgattccca aacgcgttgc gccggtattc gataactgct ccgcgtgaac gcgcgggcgc
     4501 aagccgtgct tttttccggc gagcaagacc tgacgcgatt cggcgcgtga aaatgcgctg
     4561 cgctcacaaa aaacatcgcc atactcggca agaccgtttt cggccacttc aggaatcagt
     4621 tcctgctcca gaagccggag ataatcgcgc ctgccgctgg ctttgttgcg atactcggcc
     4681 gggaccacat gggcgccaag aaatgttgaa acaatctcga gtggctgctc cgcatccagt
     4741 tctttgtgca gcgaaaggat acgcagttcg ctggttacct cgaggccgta gccgctcttg
     4801 gcctcgatcg tagttgtgcc gtgctcggca aaccgttcga gaaacatccg cgcccgcgat
     4861 tttagttctt gcgccgtctc gacgcgcagt ttgccgacgg agtcgaggat gccgccgcct
     4921 tttttggcaa tctcttcgta gccggcaccc tgaattttaa gctcgtactc ctcggcgcgg
     4981 ctggccgcgt gaatcagatg ggtgtgcgaa tctacgaagc ctggcaggac cacgcggcca
     5041 tcgaggtcca gcacttccgc ggacttgctt tccttaagct tgtcgatttt tgcggctggc
     5101 ccgacggcgg aaatcacgcc atcgcgaagc aacactgcgc catcgcgaag aacgccaatg
     5161 tcggcaagcg cttttccacg gcgcggcgcg cggccgctca ccgtcacaat ctgcgaagcg
     5221 ccgcgaatca aaagggaatt gctcacggtc cggctattat gcttgaagtc tggggcgaac
     5281 ttcaggccga tgccgctttc gcctggtacg ccggagcaaa ttcttcctgg gcgaacgctt
     5341 cgatcgaagt cggcgtggtg ttttgttcgg agcgcggctc ctggggattt agcaggccct
     5401 cgttgccggc ctcgtgcatt tcggtcatca ggctggcggt ctttttggga ataccaagtt
     5461 gtttgatgcc ttgttcgacg aggaaggccg gaaatttctg ataggagagt tttggcttcc
     5521 ca
//
LOCUS       DASTPM010000008         4923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_749931, whole genome shotgun sequence.
ACCESSION   DASTPM010000008 DASTPM010000000
VERSION     DASTPM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749931"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFR42_00115"
     CDS             <1..239
                     /locus_tag="VFR42_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367581.1"
                     /translation="WLMRMGVSTQLEQLGKEEDFRRRYVASMLPMILAGAMEHARAEM
                     GNIQVCGSGGSLQICAHRGFDRAFLEFFSGGRAR"
     gene            complement(402..1760)
                     /locus_tag="VFR42_00120"
     CDS             complement(402..1760)
                     /locus_tag="VFR42_00120"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0367582.1"
                     /translation="MPPKPHILIVDDEAGIRESLSSILTDEGYIAEAADSAERAIERA
                     SHGGLDVILLDVWLPGMDGLEALSRIQTLPHPPAVIMISGHATIDTAVRATKLGAFDF
                     IEKPLTLEKIIVLVRNAVQQRRLEQENQTLRSELGHRYQVLGDSIPMKALRQQIGVTA
                     PTNGRVLIFGESGTGKELVARSLHAASLRSKGPFVEVNCAAIPEELIESELFGHIKGS
                     FTGAVDDKVGKFQQADGGTLFLDEIGDMSLRTQSKVLRVLEEQRFEPVGSNQTVRVDV
                     RVIAATNKELEQEIAHGRFRQDLFYRLNVIPFFVPPLRDRKEDIPILARHFLNEFSSG
                     YGKRTRELSDGALEILLRYPWPGNVRELRTLVERLVIVCPQVRIEPHHLPPELFRGVI
                     ESAHQPYSTLHEARSAYEREFILRKLQENRWNMTQTTNAIGLERSHLYRKMKSLGIAA
                     PD"
     gene            1969..2967
                     /locus_tag="VFR42_00125"
     CDS             1969..2967
                     /locus_tag="VFR42_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU0367583.1"
                     /translation="MSRITGVFARSRRISACKWAVLLAVLLAPQALPAQWKAAVGAQS
                     KDLGRQALAFLPNEIWIHEGESITWTFETDEIHTVSFLEAEQVRLPFQVGCPGEMVNN
                     ASFDGSTCVTTPPLVKGQTFTVQFPLRGNYKVVCLVHENMTGVVHVLEPAATLPLEQS
                     DYDEIAAQQSKRLLADVDKDQKETSAHHHSAKHVTSGTGEIVNTAGGSQTLSVMRFLN
                     DTLVIHVGETAEWTNEDPITPHTITFGTEPANPVPPSGNVTTDADGARHATISSVFDS
                     VHSGFIVAAPQDRIGLAQASNGVTRFRITFSKAGAYLFLCALHDDLGMKGKVIVLP"
     gene            complement(3207..>4923)
                     /locus_tag="VFR42_00130"
     CDS             complement(3207..>4923)
                     /locus_tag="VFR42_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0367584.1"
                     /translation="SRCTAAEAFWQIDAAGKPFSFFDCRDETPAKLDNTLSLPPNLSP
                     SRVQPLPSGAEVWQSGGKLLLAGSARSQNGKTYYAARTVPLDFLSRFNEITRQTLAYD
                     QEKQHLRALKSQLLLILSLFTVLLLSSVLWLALYLAKQVTGPIQVLAEGTREISAGNF
                     DYQVPEQTHDELGTLVRSFNSMTTQLRDSRSQIDQFTHNLQQAVQELERRRQLIETIL
                     ENIPTGALSLDPEGNIVRINPAVSKIVGPAAKDAHTLEELVGPEDARAIRYLMRRALR
                     TGVVSRELEIIAGGRILHAAVTVSSLGPRRANSGYVVVIDDLTELLRAQKTAAWQEVA
                     RRIAHEIKNPLTPIQLSAQRLLRYLEKRQENGAASPLGDPELARLVEEAGRLIEREVG
                     ALSALVNEFSQFVRFPSAKLESADVNSIVREAIEVFSNRLDGISLNTSFAAQPPTIRA
                     DASLLRSVVVNLIDNAAEALESSPVRNITVATRANPDSDFVEIVVADTGHGISPEDKD
                     KLFLPHFSTKDRGTGLGLAIAARIVAEHGGGLRVEDNSPTGSRFILELPSAEVLASTP
                     SDRPS"
BASE COUNT          999 a         1116 c         1837 g          971 t
ORIGIN      
        1 cgtggttgat gcgcatggga gtctcgacgc aactcgagca acttggtaaa gaggaagact
       61 ttcgccggag gtatgtcgcg tcgatgctgc cgatgatcct ggccggcgcg atggaacatg
      121 cgcgcgcgga gatgggcaac atacaggttt gcggcagtgg tggatcattg cagatttgcg
      181 cgcaccgtgg attcgaccgg gcatttctgg aattcttcag cggtggcaga gcgaggtagc
      241 tgcaccggcg agagggaggc gacgaaccga caaaggatgc aggtttttgg gcgcacggag
      301 ttggtgtttt agacgaggtc cggttgaaag cgtgagcagg gatggccaga cacctcggca
      361 gatgaaaacc agaactgcgt caagctgctg cgcactccga gctagtccgg ggcggcgatg
      421 ccgagggact tcatcttgcg atagaggtgg gagcgctcga ggccgatggc gttggtggtt
      481 tgcgtcatgt tccagcggtt ttcctgaagc ttgcgcagga taaattcgcg ttcgtaggcg
      541 gagcgcgctt cgtgaagcgt gctgtagggc tggtgcgctg attcaatgac cccgcggaag
      601 agttcaggcg ggaggtggtg cggctcgatg cgcacctgcg ggcagacgat gaccaggcgt
      661 tcgacgagag tgcggagttc gcggacgttg ccgggccagg ggtaacggag aaggatttcg
      721 agcgcgccgt cggaaagttc gcgcgtacgt ttgccatagc cggagctgaa ttcgttgagg
      781 aagtggcggg cgaggatggg gatatcttct ttgcgatcgc gaagaggcgg aacgaaaaag
      841 ggaatgacgt tgagccgata gaaaagatcc tggcggaagc ggccgtgggc gatttcctgt
      901 tcgagttctt tgttggtggc ggcgatgacg cgaacgtcta cgcgcacggt ttggttggag
      961 ccgacgggtt cgaagcgctg ctcttcgagg acacggagaa ctttggattg ggtgcggaga
     1021 ctcatgtcac cgatttcgtc gaggaagagg gtgccaccgt cggcttgctg gaatttgccg
     1081 actttgtcgt cgaccgcgcc ggtgaagctg cctttgatgt ggccgaagag ttcggattcg
     1141 atgagctctt cggggatggc ggcgcagttg acttcgacga aggggccttt gctccgcagg
     1201 ctggcggcgt ggagcgagcg ggcgacgagt tctttgccgg tgccgctttc gccgaagatg
     1261 aggacgcggc cgttggtggg agcggtcact ccgatttgtt ggcgaagagc tttcatggga
     1321 atgctgtcgc cgagaacttg atagcggtgg ccgagctcgg agcgcagggt ctggttttct
     1381 tgttcgaggc gacgctgctg cacagcgttt cgaacgagaa cgataatttt ttcgagagtg
     1441 aggggctttt cgatgaagtc gaatgcaccg agtttggtgg cgcggaccgc agtgtcaatg
     1501 gtggcgtggc cggagatcat gatgacggcg ggaggatgag gaagagtctg aatgcgggag
     1561 agagcttcca ggccgtccat gccagggagc cagacgtcga gcaggatgac gtcgaggccg
     1621 ccgtgggagg cgcgttcgat ggcgcgttcg gcggagtcag cggcttcggc gatgtagcct
     1681 tcgtcggtga ggatggacga gagagattcg cggatgccgg cttcgtcgtc gacgatgagg
     1741 atgtgaggtt tgggaggcat ctttctgagg tcggcagcaa agtctgcaag gtacaaagta
     1801 gcgcagatga agagaggaat caaaattgga acgtgttgga gccgcggcag ggtaccaccg
     1861 gaaggtgggt actggcggtt cgaaccgtaa gcgactactg tgtgcaagcc ggtgcagccc
     1921 gtgaacatga gctgaggcca acccaggaca gaaagacgga gaaaataaat gagcaggatt
     1981 acgggagtgt tcgcccgcag ccggcggata agcgcgtgta agtgggcagt gttgctggcg
     2041 gtgctgctag cgccgcaagc gttgcccgcg cagtggaaag ccgcagtggg agcgcaaagc
     2101 aaggatctgg gaaggcaggc tctggcgttt ttgccgaatg aaatctggat tcacgaaggt
     2161 gagagcatca cctggacgtt cgagacggac gaaattcaca cggtgagctt tcttgaggcg
     2221 gagcaggtgc ggctgccgtt ccaggtggga tgcccaggcg agatggtgaa taacgcgagc
     2281 tttgacggtt cgacttgcgt gaccacgccg ccgctggtga aagggcagac gttcacggtg
     2341 cagtttcccc tgcggggaaa ttacaaagtg gtttgtctgg tgcacgaaaa catgaccggc
     2401 gtggtgcacg tgctggagcc ggcggcgacg cttccgctgg aacagtcgga ttacgacgaa
     2461 attgccgcgc agcagagcaa gcgtttgctt gcagacgtgg acaaggatca gaaggaaacg
     2521 tcggcacacc atcactctgc gaaacacgtg accagcggga ccggagaaat tgtgaatacg
     2581 gcgggaggat cgcagacctt gtcggtgatg cgttttctca atgacacgct ggtgattcac
     2641 gtgggggaaa cggcggaatg gacaaatgag gatccgatca cgccgcacac gatcaccttc
     2701 ggcacagagc cggcgaatcc ggtgccgcca tcggggaatg tgactaccga tgcggacgga
     2761 gccagacacg ccaccatcag ctcagtattc gacagtgtcc actccgggtt cattgtggcg
     2821 gcgccgcagg accgcatcgg tctggcgcaa gcgagcaatg gcgtgacgcg cttccggatt
     2881 accttcagca aggcgggggc gtatctattt ctttgtgccc tgcatgatga tttggggatg
     2941 aagggaaaag tgatcgtact gccttgacgc ggtcacgatt cgaaatggaa aagcggcccg
     3001 gattttccgg caatgttgcc ggggattcgg gccggttttt tgtgcgagag cggaagagtt
     3061 atgaggcgtt gaggttgaaa tcgctctgag ctgctcttta ggcggtagtt tctccgggcg
     3121 gctcctttcc ggggcccacc tcagggcggc cgctacaagg gagaggctgg gcgtatgcga
     3181 ggaaagagat aagcctagaa agaattttag gaggggcggt cggagggggt ggaggccaaa
     3241 acttcggcgg aggggagttc gaggatgaag cgggagccgg tgggggagtt gtcttcgacg
     3301 cgcaggccgc caccatgctc ggcgacgata cgcgcggcaa tggcgaggcc gaggccggtg
     3361 ccgcgatctt tggttgagaa gtggggaaga aagagtttgt ctttgtcctc cggggagatg
     3421 ccgtggccgg tgtcggcgac gacgatttcg acgaagtcgg aatcgggatt ggcacgggtg
     3481 gctacggtga tgttgcggac aggagacgat tcgagggctt cggcggcgtt gtcgatcagg
     3541 ttgacgacaa cagagcggag cagggaagca tcggcgcgga tcgtgggagg ctgcgcggcg
     3601 aaggaagtgt tgaggctaat gccgtcgaga cgattggaga agacttcgat ggcttcgcgg
     3661 acgatggagt tgacgtcggc ggattcgagt ttggcggaag ggaagcggac gaactgggag
     3721 aattcgttga cgagcgcgga cagagcgccc acctcgcgct cgatgaggcg gccggcttcc
     3781 tcaaccagac gagctagctc gggatcgcct aatggggaag cggcgccatt ttcctggcgc
     3841 ttttcgaggt agcgaagcag gcgctgggcg gagagttgga tgggagtgag cggatttttg
     3901 atttcgtggg cgatgcggcg cgcgacttct tgccatgcgg cggttttttg cgcgcggagg
     3961 agttcggtga ggtcatcaat cacaacgacg tagccggagt tggcacggcg tggaccgagg
     4021 gagctcacag tgacggcggc gtgaaggata cggccgccgg cgatgatttc gagctcgcgg
     4081 gagacgacgc ccgtgcggag agcgcggcgc atgagatagc ggatggcgcg agcgtcttcg
     4141 gggccgacaa gctcttccag cgtgtgggca tccttggcgg cggggccgac aatttttgag
     4201 acggcgggat tgatgcggac gatgttgcct tcggggtcga gcgagagggc tccggtggga
     4261 atgttttcaa ggatggtttc gatgagctgg cggcggcgtt cgagttcctg cacggcttgc
     4321 tgcaggttgt gggtgaactg atcgatttgc gagcgggagt cgcggagctg ggtggtcatg
     4381 ctgttgaagg agcggaccag ggttccaagt tcgtcgtggg tctgctcagg aacctgataa
     4441 tcgaaatttc ctgcggagat ctcgcgtgtg ccttccgcga ggacctggat aggtccagtg
     4501 acttgtttgg cgagataaag agcgagccaa aggaccgagg acaggagaag gactgtaaac
     4561 aaggagagga tgagcaagag ctggctcttc agagcgcgga ggtgctgctt ttcctgatcg
     4621 taggcgagtg tctgacgcgt gatttcattg aaacgagaga gaaaatcgag gggaaccgtg
     4681 cgggccgcgt aataggtttt gccgttttgc gagcgggcgg aacctgcgag aaggagcttg
     4741 ccgccggatt gccagacttc cgcgccgctg gggagcggct gaacgcgaga aggggagaga
     4801 ttcggtggaa gggacagcgt gttgtcgagc ttcgcgggag tttcgtcgcg acaatcgaaa
     4861 aaggaaaatg gcttgccggc cgcgtcgatt tgccagaaag cctcggcggc ggtgcaacgg
     4921 gaa
//
LOCUS       DASTPM010000009         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_144537, whole genome shotgun sequence.
ACCESSION   DASTPM010000009 DASTPM010000000
VERSION     DASTPM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144537"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFR42_00135"
     CDS             complement(<1..381)
                     /locus_tag="VFR42_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0367585.1"
                     /translation="MRSPAQFLFLATLMFASQTNSQSSQPLNLMPQPADVNLGSGALK
                     IDVSFTIALAGHTEPRLDRAATRFLAQLQRQSALLFAPQPSRSAAKATLVIHTDHASK
                     DVQELGEDESYTLEVTTSGAKLTAA"
     gene            609..1091
                     /gene="coaD"
                     /locus_tag="VFR42_00140"
     CDS             609..1091
                     /gene="coaD"
                     /locus_tag="VFR42_00140"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU0367586.1"
                     /translation="MKTSIAIYPGSFDPVTNGHLDLIERGEKMFDLLIVAVLQNAEKR
                     PLFSVSERVEMLRETTKQWNDVEIDVFDGLLVDYARKKGAGVILRGIRAVSDYEYELQ
                     MALMNRKLEPRLETVFMLPGLTYSYLSSKLVREIAQLGAPLKGLVPAIVEERLRAKVS
                     "
     gene            1177..2337
                     /locus_tag="VFR42_00145"
     CDS             1177..2337
                     /locus_tag="VFR42_00145"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0367587.1"
                     /translation="MSPTSAVIAAADQLKSKGVDIADFGPGEPDFPTPDHIKQAAIEA
                     IHGNKTKYTPTGGIMPLREAICAWHKRELKSNYEPKECVVSVGGKHAIFNVASVLVQA
                     GDEVLIPAPYWVSFPDIVKYTGATPVYVQTSVEDGFAVRAEAIEKAITPKTRLLILNS
                     PSNPSGGVTPPDEYEKILAVCKRHNVWLMGDECYSHFTYDPHKPFSIASAKDSKENVI
                     IIGSVSKTFAMTGWRIGYALGPAALIQALVKLQSQSTSNPTSIAQYAALAAMTGTMET
                     VPVMLAEYAKRRKRVVEGLNAIPGVKCGQPGGAFYAFPDVSAHLSGPHALARDTTELA
                     TLLLDKARVALVSGDAFGAPGYLRISYATSLDRIEEGLRRLDRFFSRAEAAA"
     gene            2341..>3162
                     /locus_tag="VFR42_00150"
     CDS             2341..>3162
                     /locus_tag="VFR42_00150"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEU0367588.1"
                     /translation="MIPEPFRIEPTFSPRIWGARSLAPIFPDKKDLAEPIGEAWLTDV
                     RCQVASGEFQGMSLGEAWRYMPVEWRGERCAATQDFPLLVKFIFPKDKLSIQVHPDDE
                     YASKHEAAAGGRGKTEMWHVVSAEPDAQVFVGLKPGTTKKEFAGATGKRGLEELFQPH
                     TVEARETFFIPPGIPHTIGPGMILCEVQQYSDLTYRVYDYGRVDAAGKPRELHIEKAL
                     DVIRFGHPAIHRMQPLSWSSQMMTISLLVACRYFSTERWRIAEHFTARPKTDAFNL"
BASE COUNT          663 a          715 c         1133 g          651 t
ORIGIN      
        1 ggcagcggtt agcttggcgc cggaagttgt gacttcgagc gtgtaggatt cgtcttcgcc
       61 gagttcctgg acgtctttgc tggcatgatc cgtgtggatg acgagggtgg ctttggcggc
      121 gctgcgcgag ggctgcgggg cgaaaagtaa ggcggactgg cgctggagtt gtgcgaggaa
      181 gcgagtggcg gcgcggtcga ggcgaggctc agtgtggccg gcgagtgcga tggtgaagct
      241 gacgtcaatt tttagagcgc ctgagcccaa gtttacgtcg gccggttggg gcatcaggtt
      301 caacggttgc gaggattgcg agttggtttg tgaagcgaac atgagggtgg caagaaaaag
      361 gaattgggcc ggtgaccgca tgatgaacga agtcctccgt cgcaggaagt cggaatccgc
      421 gcgcctgggg tgctatgtag cagcggaaga gggatattgg cacactcttt tcggaatgcg
      481 aagaacaaat tggcggtctg ggttgtgggc ggggccaaag tagatgtttg gctggcgtaa
      541 gtacatgcga gttttgtgag tcggtgaagg tttctcgtgc gctacactaa gggtggttca
      601 ggaagccgat gaagacctcg atcgccattt atccgggttc gtttgatccg gtgaccaacg
      661 gccacctcga cttgatcgag cgcggcgaga agatgtttga tttgctgatt gtggcggtgc
      721 tgcagaacgc ggagaagcgg ccactgtttt cggtgagtga acgggtggag atgctgcgcg
      781 agacgacgaa gcagtggaat gacgtagaaa tcgatgtgtt tgacgggttg ctggtggatt
      841 acgcacggaa aaaaggcgcg ggcgtgattt tacgtggcat tcgcgctgtg agcgattacg
      901 agtacgaact gcagatggcg ctgatgaacc gcaaactgga gccccggctg gagacggtat
      961 ttatgctgcc ggggttgacg tacagctatt tgagttcgaa gctggtgcgg gagattgcgc
     1021 agctgggggc gccgctaaag gggctggtgc cggcgattgt tgaggagcgg ttgcgggcaa
     1081 aggtttcgta gaagaaggcg ttcaaaccgg cgtgagagag cgccgttacg actaggagaa
     1141 gcagtgaggc tgaccgatcg aattaatcga atacaagtgt cgccgacgtc cgccgtgatt
     1201 gcggcggcgg atcaattaaa gtccaaggga gtggacattg cggacttcgg tccgggcgag
     1261 ccggattttc ctacgccgga tcacatcaag caggcggcaa ttgaagcgat tcacgggaac
     1321 aagacgaaat acacgccgac gggtggaatc atgccgctgc gcgaagcgat ttgcgcgtgg
     1381 cacaagcggg agttgaagtc gaactacgag ccgaaggaat gcgtcgtgag cgtcggcggc
     1441 aagcacgcga tttttaatgt ggcgagcgtg ctggtgcagg ctggggacga agtgctgatt
     1501 cccgcaccgt actgggtgag ctttccggac atcgtcaagt acacgggcgc cacgccggtg
     1561 tatgtgcaga cgagcgtgga ggacggattt gcggtgcggg cggaggcgat tgaaaaggcg
     1621 attacgccaa agacgcggct gctgattttg aactcgccga gtaatccgag cgggggagtg
     1681 acgccgccgg acgagtacga aaagattttg gcggtctgca aaaggcacaa cgtctggctg
     1741 atgggcgacg agtgctattc gcactttact tatgatccgc acaaaccgtt ctcgattgcc
     1801 agcgcgaagg attcgaaaga gaacgtgatt attatcggct cggtgtcgaa gacgtttgcg
     1861 atgaccggat ggcgcattgg ttacgcgctg gggccggcgg cgctgattca agcgctggtg
     1921 aagctgcaga gccagtcgac gtcgaatccg acttcgattg cgcagtatgc ggcgctggcg
     1981 gcaatgaccg gaacgatgga gacggtgccg gtgatgctgg cggaatatgc gaagcggcgg
     2041 aagcgggtcg tggaaggact gaatgcgatt cccggcgtga agtgcgggca gcccgggggg
     2101 gcattttatg cgtttccgga tgtgtcggcg catttgagcg ggccgcatgc gctggcgagg
     2161 gatacgacgg aactggcgac gctgctgttg gataaagcgc gcgtggcgct ggtgagcggc
     2221 gatgcgttcg gcgcgccggg atatttgcgc atttcgtacg cgacttcgtt ggaccggatt
     2281 gaggagggac tgcggcgcct cgaccgattc ttttcccgcg ccgaggcagc agcctgattc
     2341 gtgattccgg aaccgtttcg aattgagccg acgttcagtc cccggatttg gggcgcgcgt
     2401 tcgctcgcgc cgattttccc agataaaaag gacttagctg agccgattgg cgaagcatgg
     2461 ctaacggacg tgcgctgcca ggtggcgagc ggggaattcc aggggatgag cctcggcgaa
     2521 gcgtggcgct acatgccagt ggagtggcgg ggcgagcggt gcgctgcgac gcaggatttt
     2581 ccgctgctgg tgaaattcat ttttccgaag gacaaactgt ctattcaagt acatcccgac
     2641 gatgaatacg ccagcaagca tgaagcggca gcgggtggac gcggaaagac ggagatgtgg
     2701 cacgtggttt cggctgagcc ggacgctcag gttttcgtcg ggctgaaacc ggggacaacg
     2761 aaaaaagagt ttgcgggggc gaccggtaag cgcggattgg aagagttgtt tcaaccgcat
     2821 acagtggaag cgcgcgagac gttttttata ccgccaggaa ttccgcacac gatcgggccg
     2881 ggaatgattt tgtgcgaggt acagcaatat tcggacctga cgtatcgcgt ttacgattac
     2941 ggccgggtgg atgcggcagg caagccgcgc gagctgcata ttgaaaaagc gctggacgta
     3001 attcggtttg ggcatccggc gattcaccgg atgcagccgc tgagctggtc gtcgcagatg
     3061 atgacgattt cactgttagt ggcgtgccga tatttttcca cggaacgctg gcggattgcg
     3121 gaacacttca cagcacggcc caaaaccgat gcgttcaatc ta
//
LOCUS       DASTPM010000010         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_173396, whole genome shotgun sequence.
ACCESSION   DASTPM010000010 DASTPM010000000
VERSION     DASTPM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173396"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..603)
                     /locus_tag="VFR42_00155"
     CDS             complement(235..603)
                     /locus_tag="VFR42_00155"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEU0367589.1"
                     /translation="MSNKPPSHDAIKKRLSRLNEITITVTGRKSGRAISVPVWFVLED
                     AKLYLLPVSGSDTQWYKNVLENSALRIDAGGDTAALQPIPITDPKQVASVVEKFRAKH
                     GTADVKKYYSKFDVAVVAEL"
     gene            complement(676..1266)
                     /locus_tag="VFR42_00160"
     CDS             complement(676..1266)
                     /locus_tag="VFR42_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367590.1"
                     /translation="MPRKFAPIFITFLLCAGAPRLFAQQRVEAGLFIDYLGISQTGTS
                     NFGLGGRFGYRVHRKVMLEGELAYDYGINFQEAYTNISNGNIAAIGRTSIGVTHGLFG
                     PALQPARGRLRPFVTMKAGFIDFRLSPSLLPLTSFESTLLGIRTSSLNAAVYPGAGVE
                     ATLGPLGLRLEFGDEIYFNHGAHNNLRITFGPIIRF"
     gene            complement(1383..>2243)
                     /gene="hemB"
                     /locus_tag="VFR42_00165"
     CDS             complement(1383..>2243)
                     /gene="hemB"
                     /locus_tag="VFR42_00165"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0367591.1"
                     /translation="GSKVRQEVSSMPDTFQQSADQIVEECREVESLGIPGVILFGLPE
                     SKDPRGASSLSTQGVVQRAIEAIRKANLKLLVITDVCLCEYTDHGHCGVIENGDVAND
                     ATVEILADQALSHARAGADIVAPSDMMDGRVAAIREKLDQHKLENVAILSYAAKYCSG
                     FYGPFREAAQSAPQFGDRRSYQMDPANAREALKEVALDLEEGADMVMVKPALPYLDVI
                     RAVREKFDVPVGAYNVSGEYAMVKAAAAKGWLDEKRVALEILTGIQRAGASFTLTYHA
                     KQAARWLKHC"
BASE COUNT          447 a          577 c          771 g          448 t
ORIGIN      
        1 agggggagga tcggcaacag gaggaagaaa ccaaagaggg gttcttcgct gcgctcagaa
       61 tgacaaggcg ggggaggggt tcttcgctat cgctcagaat gacggcggaa gaggggttcg
      121 tcgctggcag aaggcgcgcg gcgtcggcag gtcatcctga ggctctggcc gaaggatctc
      181 tcccgagggt tgggggagaa gagggattct tcgctgcgct cagaatgaca gctactacag
      241 ctcagcgacg acggcgacgt cgaatttgga gtaatacttt ttcacgtcgg cggtgccgtg
      301 cttggcgcga aatttttcca cgacggaggc gacttgtttg gggtcggtga tgggaattgg
      361 ttggagcgcg gcggtgtcgc cgccggcgtc gatgcgcagc gcggagttct cgagcacgtt
      421 cttgtaccac tgggtgtcgg agccgctgac cggcaaaaga tagagcttcg cgtcttccag
      481 gacgaaccac accgggacgg agatggcgcg cccggatttt cgcccggtca ccgtgatggt
      541 gatctcgttc aagcgcgaca gacgcttttt gatggcgtcg tgcgatgggg gcttgttgct
      601 catgggaacg tcctcgactc cagttggatg ccgcgcagcc cggcaagttg cggacaagcc
      661 agccgccggc gaacgtcaga acctgataat tggaccgaag gttatgcgca gattattgtg
      721 cgcgccatga ttaaagtaga tttcgtcgcc aaattccagg cgcaagccca gcgggccgag
      781 agttgcctcc acgcctgctc cgggatatac ggccgcgttc aagctggagg tgcgaatgcc
      841 gaggagcgtg ctctcaaacg aagtcagcgg gagcaggctg gggctcaggc gaaaatcgat
      901 gaagccggct ttcatggtga cgaaagggcg aaggcggccc ctcgcgggct ggagggcagg
      961 cccaaacagg ccatgcgtga cgccgatcga tgttcgtccg atggcggcaa tattgccgtt
     1021 gctgatgttg gtgtaggcct cctggaagtt gatcccgtag tcgtaggcga gttcgccttc
     1081 gagcatcact ttgcggtgaa cgcggtagcc aaagcgtccg ccgagcccaa aattgctggt
     1141 gccggtctgg gaaatgccga ggtaatcgat aaacaggccg gcttcgacgc gctgctgcgc
     1201 gaacaagcgg ggagcacctg cgcacaaaag aaacgttatg aagatgggag cgaatttgcg
     1261 cggcacagcg tgcacctccg gaggatcacg acctggccgg gcatagatga gatgaagcga
     1321 gccttgctaa aggcgtagac gaatcgatga gcgggagccg gtgagatagg cgctgcgagg
     1381 cgctagcagt gctttagcca gcgggcggct tgcttggcgt ggtaggtgag ggtgaaggag
     1441 gcgccggcgc gctggatgcc ggtgaggatt tcgagggcga cgcgtttttc gtcgagccag
     1501 cctttggcgg cggcggcttt gaccatggcg tattcgccgc tgacgttgta agcgccgacg
     1561 ggcacatcga atttttcgcg gacggcgcga atcacgtcga gatacggaag cgcgggcttg
     1621 accatgacca tgtcggcgcc ttcttccagg tcaagcgcaa cttctttcaa cgcttctcgg
     1681 gcgttggcgg gatccatctg gtagctgcgg cgatcgccaa attgaggagc ggattgcgcg
     1741 gcttcacgga acgggccgta gaatccggag caatatttgg cggcgtagga aagaatggcg
     1801 acgttttcca atttgtgctg gtcgagtttt tcgcggatgg cggcgactcg gccatccatc
     1861 atgtcagagg gcgcgacgat gtcggcgccg gcgcgcgcgt gagaaagagc ctggtcagcg
     1921 agaatttcga cagtggcgtc gttggcgacg tcaccatttt cgatgacgcc gcagtggccg
     1981 tggtcggtgt attcgcacag gcagacatcg gtaatgacca aaagttttaa gttcgctttg
     2041 cgaatggctt cgatggcgcg ctgcaccacg ccttgcgtgg aaagcgaact ggcgccgcgc
     2101 ggatccttcg attccggcag accaaagaga atgactccgg gaattcccag cgattccact
     2161 tccctgcact cttccacaat ttgatcggcg gattgctgga aagtatccgg catggagctg
     2221 acttcctggc gaactttgct gcc
//
LOCUS       DASTPM010000011         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_692438, whole genome shotgun sequence.
ACCESSION   DASTPM010000011 DASTPM010000000
VERSION     DASTPM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692438"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFR42_00170"
     CDS             <1..425
                     /locus_tag="VFR42_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367592.1"
                     /translation="AGVVGTDFFVSFDNTTGTMNVTVFEGVVKVCNLAGVCVEVKAGQ
                     FSNVRTNDSTGPQPPTQATLDVLMSAGSTTEVGGGPGVQNVGHHMGKGTAITLGILAA
                     IPAIVIPVVVARGNNGSTPPQVGKPSGGCSTNPNGPNC"
     gene            complement(500..2668)
                     /locus_tag="VFR42_00175"
     CDS             complement(500..2668)
                     /locus_tag="VFR42_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0367593.1"
                     /translation="MLSRHPTAATLVALLAATSAAISLRAQDASPATPARFADWPRYG
                     GTPENNHYSALAQINRENVGRLQVAWQFDTGEEGGLQTTPIIVHGVLYGITPTQKIFA
                     LDAATGKPLWKFDSGIVGTQPDRGLTFWQSPSVDGAVKDARILVGVLNYLYALDAATG
                     KPVATFGDNGRIDLRENLGREPASANSIYLTSPGVVFEDLIIVGGRMPETLPAPPGDV
                     RAFDVRTGKLRWAFHTIPRPGEAGYETWPKDAWKTSGAANNWTGMALDSARGIVYVPT
                     GSAASDFYGTDRAGDNLYANCLLALDARTGKRLWHFQAVHHDLWDRDFPSPPVLLTVQ
                     RDGQSVAAVAQTSKQGFVYLFNRSTGKPLFPLETKAYPASTVPGENAAKEQSLPTKPA
                     PFARQLLTEDMLTERTPEAHRWALENFKLFRSEGQFVPLSTRKETIVFPGFDGAAEWG
                     GPAVDPQTGVIYINSNEMAWNAALGESTDATGTRALYTSQCSICHHDDLKGAAPDFPS
                     LDGIGARLTFSQIAATIHNGKGRMPGFPNLTPEETSAIADFLVNGRSVDRSAAASDKE
                     IVSSGPQLPAMKYHLMGYNRFLDPDGYPAIAPPWGTLNAINLNTGEYLWKIPFGEYPE
                     LTAAGMKDTGSENYGGPVVTAGGLLFIGASNFDKKFRAYNSSTGALLWETTLPFSGNA
                     TPATYSIRGRQFVVIAAGGGKDRKSKSGGVYVAFFLPDGI"
BASE COUNT          529 a          726 c          976 g          551 t
ORIGIN      
        1 tggcgggcgt ggtgggtacg gatttctttg tgagcttcga caacaccacg ggaaccatga
       61 acgtgactgt gttcgaaggt gtggtgaagg tttgcaattt ggctggcgta tgtgtggagg
      121 tgaaggccgg ccaattctcc aacgtgcgca cgaatgacag cacaggcccg cagccgccga
      181 cgcaagcgac gctggacgtg ctgatgagcg cgggatcgac cacggaggtt ggcggcggac
      241 cgggcgtgca gaacgtggga catcatatgg gcaagggtac ggcgattacg ctggggattc
      301 tggcggcgat tccggccatt gtgatcccgg tggttgtggc gcggggaaac aacgggtcaa
      361 ctccgccgca agtgggcaag cccagtggcg gatgctctac aaatccgaac gggcctaatt
      421 gttgagggac gtcagctgag cgtagagagg gcctgcgcac tttgacgcaa gagcgtcgaa
      481 gcctctcgat gacgaatttt tagatcccat cgggaagaaa aaaagccaca tagactccgc
      541 cggatttgga cttgcggtcc ttgccgccgc ccgcggcaat cacgacaaat tgtcggcccc
      601 tgatggagta ggttgcgggc gtagcgttgc ctgaaaacgg cagcgtggtc tcccagagca
      661 atgcgcctgt ggaactgttg taggcgcgga attttttgtc gaagttgctg gcgccgatga
      721 agagcagtcc gcccgcggtg accacgggcc cgccgtaatt ttcgctacca gtatctttca
      781 tgcccgccgc ggttagttcg gggtactcgc cgaacggaat cttccagaga tattcgccgg
      841 tattcagatt gatggcgttg agcgtgcccc atggcggcgc gatggccgga tagccatcgg
      901 gatcgaggaa gcggttgtag cccatgaggt ggtacttcat ggcaggaagc tgcgggccag
      961 agctgacaat ttccttatca gaggcggctg cggagcggtc aacggaccga ccgttcacca
     1021 agaagtccgc gatggcgctg gtttcctcag gcgtgagatt gggaaagccc ggcatgcgcc
     1081 ccttgccatt gtgaatggtt gcggcgatct ggctgaaggt gaggcgcgcg ccgattccat
     1141 caagcgacgg aaaatcgggc gccgcgccct tgaggtcgtc gtgatgacaa atgctgcact
     1201 gcgaggtgta aagcgcgcgc gttccagtgg cgtcggtgga ttcgccgaga gccgcgttcc
     1261 aggccatttc gttggagttg atatagatga cgccggtttg tggatcgacc gcgggaccgc
     1321 cccactccgc ggctccgtcg aagcctggga aaacgatggt ttctttacgc gtggagagtg
     1381 gaacgaactg gccttcgctg cggaacagct tgaagttctc gagcgcccaa cggtgggctt
     1441 cgggcgtgcg ctcggtgagc atgtcttcgg tgagaagctg gcgcgcgaaa ggcgctggct
     1501 tggtgggcag cgattgttcc ttcgcggcgt tttcgccggg cacggtgctg gccggatagg
     1561 ctttcgtttc aagcgggaag agcggtttac ccgtgctgcg gttgaagaga tagacgaagc
     1621 cctgcttgga agtttgcgcg accgcggcga cggactggcc atcgcgttga acggtgagta
     1681 gtaccggcgg cgacgggaag tcgcggtccc agaggtcatg gtgcacggcc tggaaatgcc
     1741 aaaggcgctt gccggtgcgc gcgtcgagcg cgagcagaca gttggcgtat agattgtcgc
     1801 cggcgcggtc ggtaccgtag aaatccgatg cggccgaacc ggtcggaacg taaacgatgc
     1861 cgcgcgccga gtcgagcgcc ataccggtcc aattgttggc ggcgccgctg gtcttccagg
     1921 cgtccttcgg ccaggtttcg tagccggcct cgccgggtcg cggaattgtg tggaatgccc
     1981 agcgcaattt gccggtgcgg acatcgaagg cgcgaacatc gccgggaggc gcgggcaaag
     2041 tttccggcat gcggccgccg acgataatta ggtcttcaaa aacgacgccc gggctggtga
     2101 ggtagatgga gttggcggag gcaggttcgc ggccgagatt ttcgcgcagg tcgatgcggc
     2161 cgttgtcgcc aaaggttgcg acaggtttgc cagtggcggc atcgagcgcg tagagataat
     2221 tcagaacccc gaccaggatg cgagcgtctt tgacggcacc atcgacggag ggcgattgcc
     2281 agaaggtcag gccccgatcg ggctgtgtgc cgacaatgcc ggaatcgaat ttccagagcg
     2341 gttttccggt agccgcatcg agcgcaaaga ttttctgggt cggagtgatt ccgtagagca
     2401 cgccatggac gatgatgggg gtggtctgca gaccgccttc ttcgcccgtg tcgaactgcc
     2461 aggccacttg caagcggccg acattttcac ggttgatctg tgccaacgca gagtagtggt
     2521 tattctccgg cgttccgccg taacggggcc agtcagcgaa tctggcggga gtcgcggggc
     2581 ttgcgtcttg cgcgcgcaaa gaaatcgccg ctgacgtggc ggcaagcaag gcgacaagcg
     2641 tggcggcggt agggtgtcgg gagagcacgt ttttttcctt ggcgcaaaaa accggttacg
     2701 gtgcgtcgaa ccagagaggc gttcggacca ctttgcggcc cctcagggtg aaggagtgta
     2761 tcgcaggtgt cgatggaatt ca
//
LOCUS       DASTPM010000012         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_115813, whole genome shotgun sequence.
ACCESSION   DASTPM010000012 DASTPM010000000
VERSION     DASTPM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115813"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..876)
                     /locus_tag="VFR42_00180"
     CDS             complement(148..876)
                     /locus_tag="VFR42_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367594.1"
                     /translation="MITTNTKIFRIGCVAAFLLASLSLSERTNAQAPPGPLPGTAAQD
                     SASAPAAKAPAPNPRIKNLAGTWRLNADDSDDPRKKLQQARGGGRGQGGGRSGGGMGG
                     GWPGMGGHGGYGGHRQGGESDEERQKMLLFLQPAQRLNIAQKEPEIDINDDSDRKFTA
                     FTDGRKVEKSKDPGTQQFDAKWDDYRLVMEGKDPRGNKYQRSYEVLEGNQQLRETFFL
                     KVGRNNTEISIRYVYDLVSPPKKS"
     gene            complement(964..1734)
                     /locus_tag="VFR42_00185"
     CDS             complement(964..1734)
                     /locus_tag="VFR42_00185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0367595.1"
                     /translation="MEVAVVTGAAQGIGRRTAEILAERGFALALIDLREPSETLAALK
                     SRNADALSFAADITKETAISEFARAVESRWGRVDVLVNNAGVSFIAPAETMSAKDYRR
                     VLEVNLVAPFLLAQAFGKKMLAAGQGSIINIASVAGLLGIADRAAYNASKHGLIGLTR
                     TLAAEWGGRGVRVNAVCPGWVKTEMDVRDQTGGGYADADITGRVPMGRFASPDDIARA
                     VAFLADDDASAFINGHALAVDGGWTADGSWESLRLRHR"
     gene            2064..>2286
                     /locus_tag="VFR42_00190"
     CDS             2064..>2286
                     /locus_tag="VFR42_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367596.1"
                     /translation="MLIAWGIVTAVFLALLIWRNLLESHEDDQLFLDAAEEHMAKEQR
                     ALVQRINTLSKPIMMTGILAGILLLASGGL"
BASE COUNT          411 a          652 c          727 g          496 t
ORIGIN      
        1 agcagcggct ccatgggggg cgagcaaagc tggattccag cgccgaattg gttagttccg
       61 tgggctgggc tgcgcgagag aatctacaac ttccgcacaa gcggatatgg agacggttcc
      121 tctgccagac gccatcgcga agtggcttca ggacttttta ggaggcgaca ccaggtcgta
      181 gacgtagcgg atggagattt cggtattgtt gcggccgacc ttgaggaaga acgtttcgcg
      241 gagctgctgg ttgccttcga gcacttcgta ggagcgctgg tatttgttgc cgcgcgggtc
      301 tttgccttcc atgacgaggc ggtagtcatc ccacttggca tcaaactgct gtgtgccggg
      361 gtcttttgat ttttcaactt tgcggccgtc ggtgaaggca gtgaatttgc ggtcggagtc
      421 gtcgttgatg tcgatttccg gctctttttg agcgatgttc aaccgctgcg caggctgcag
      481 aaacagcagc atcttctgac gctcttcgtc gctctcgccg ccttgccggt gtccaccgta
      541 gccgccgtga ccgcccatgc ccggccagcc accgcccatt ccaccgccgg atcggccgcc
      601 gccctgtccg cggccaccgc cgcgggcctg ctgtagtttc ttgcgcggat cgtcgctgtc
      661 atcggcgttg agcctccagg tgccggcaag attcttgatg cgcggattgg gcgccggagc
      721 cttcgctgcc ggagcagatg ccgagtcttg cgctgcggtg cccggcagcg ggccgggagg
      781 cgcttgggca tttgttctct cggaaaggct gagggaagca aggaggaaag ctgcgacgca
      841 gcctatacgg aaaatttttg tgttggtagt aatcaccgat tgcccctatt tcgggattcc
      901 cgcgactggg cgggaaagtt cccctaagcg cgtgcctcac ttgattggat gcgaagtgga
      961 ttgtcagcga tggcgcaggc ggagggattc ccaactgcca tcggcggtcc agccgccgtc
     1021 gaccgcgagg gcgtggccgt tgataaaggc gctggcgtcg tcgtcggcaa gaaatgccac
     1081 ggcgcgggca atgtcgtcgg gagaagcgaa acgtcccatg ggcacgcggc cggtgatgtc
     1141 ggcatcggca tagccaccgc cggtctggtc tctaacgtcc atttcggttt tgacccagcc
     1201 ggggcaaacc gcgttgacgc ggacgccgcg gccgccccac tcggcggcga gcgtgcgcgt
     1261 gagtccgatc agtccgtgct tggaagcgtt gtacgcggcg cgatcggcga tgccaagcaa
     1321 gccggcaacc gaggcaatgt tgatgatcga accttggccg gcggccagca tttttttgcc
     1381 gaaggcttgc gccagcaaga atggagcgac cagattgact tcgaggacgc ggcgatagtc
     1441 tttcgcactc atagtttcgg cgggcgcgat gaagctgacg ccggcattgt tgacgagcac
     1501 atcaacgcgg ccccagcgcg attccacagc acgggcgaat tcgctgatgg cagtttcctt
     1561 ggtgatatcg gcggcaaagc tcagggcatc cgcgtttcgg cttttcaacg cagccagtgt
     1621 ttccgatggc tcgcgcagat cgatcagcgc cagagcaaat ccacgttcgg ccagaatttc
     1681 ggcggtgcgg cggccgatgc cctgcgctgc gccggtgaca acggcaactt ccattggcgg
     1741 tggctccttc ttaggttttg gtcgcgacat tcctaatcgt aacagggagc gatgggagga
     1801 aacgggcggc gaaagcgaaa accaggttgt gcatttattg ctctaaactc ttcaaaatgt
     1861 gccgtttgtg gcgttccgcc gcaaggatgc cttgcagcaa aaccgctact aggatgtgct
     1921 ggtttttttc attgttggtg ctatactccg ccgcagaagc cgtcccaccc cttggcaggt
     1981 cttgccaaaa gcctgccacc gcggttcggt tccaacagga ggtcttaatg tcggaatcaa
     2041 cgcttacggg gcctgtcctt tacatgctga tcgcctgggg gatcgtcacc gccgtatttc
     2101 tggccctgct catctggcgg aacttgctgg agagccacga ggacgatcaa ctctttctcg
     2161 atgcagcaga agaacacatg gccaaggaac agcgtgcgct ggtgcagcgt atcaacacgc
     2221 tgtcgaagcc gatcatgatg accggcattc tggcgggaat cctgctgctc gcctccggcg
     2281 gtctct
//
LOCUS       DASTPM010000013         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_605972, whole genome shotgun sequence.
ACCESSION   DASTPM010000013 DASTPM010000000
VERSION     DASTPM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605972"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFR42_00195"
     CDS             <1..357
                     /locus_tag="VFR42_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367597.1"
                     /translation="GKLSKKLTDGYRSIVKKVGLTAYVASAGVNGALMLYPQEIKNYR
                     DWTKIDIDLWRHYWFAMVNRGVLAQPYWWDEQWTISVQHSEADIDKHLAVFEEIAPGL
                     AKAQAERGMVTVAAAH"
     gene            494..1912
                     /locus_tag="VFR42_00200"
     CDS             494..1912
                     /locus_tag="VFR42_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0367598.1"
                     /translation="MTQEVNTAESHPHLKRVLGQRDLVLLFVVAVFNLNVVPSIAANG
                     GVTIWLWIISLILFFWPQGIAVIELAHRYPGEGGVYLWAKEVFGDFHGFLSGWCYWTN
                     NMMYVPTVLLYFVGVSVFALGSGHEALADNKIFALAACVVLLAVLVILNIVGLGVGKW
                     INNVGGIGTFFAAALLMGLGLIVWTRFGTTVTWSDFQIPANPRFVLNSFGVICFGLVG
                     LELASVMGDEIQDPAKTLPGAVAWGGVLSGVLYIGATLTLLVAVSKSDISVLQGIVQA
                     VTRMAGRVGVLWIVAPFAFLLSLSIAGIGSAWLGGSARIPFVAGLDSYMPRWLGSIHA
                     KYRTPYAALLVHAVVSLLLVLMNFWGSGVQESFQRMLSLAVVLQLVPFLYMFGALLKI
                     ALDQRFQPARYSKSTLLLAGASGLLTTSLGIGLAFFPAQQISSLVSYETWMVGGTIFF
                     IGAAAFFFFVYGRRKVAFKAAA"
     gene            1937..>2696
                     /locus_tag="VFR42_00205"
     CDS             1937..>2696
                     /locus_tag="VFR42_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0367599.1"
                     /translation="MSGSATTAGVRTYQNFVNGKWESAASGKTFPVYDPSTEEVIAHV
                     AAADVADVDRAVKAARAAFDSGPWPQTTAQDRGRILFRLAEEIRRNSAALAELECRNT
                     GKPIVEAEYDMADVSTCFEYYGGLANKVTGHVNPVPANALSFTMREPVGIAGQIIPWN
                     YPLLMAAWKLAPAIAAGCCCVLKPAEQTPLTALEFANYFEQAGLPPGVVNIVNGFGET
                     AGAALVAHPGVDKIAFTGSAAVGKIIVKSAADTLK"
BASE COUNT          480 a          846 c          724 g          646 t
ORIGIN      
        1 ggcaagctca gcaaaaagct caccgacggc tatcgctcga tagttaagaa agtcggtttg
       61 accgcgtacg tcgctagcgc tggagtcaac ggcgcgctca tgctctatcc gcaggaaatc
      121 aagaattatc gcgactggac caagatcgat atcgatcttt ggcgccacta ttggttcgcc
      181 atggtcaatc gcggcgtgct cgctcaaccc tattggtggg acgagcagtg gaccatttcc
      241 gtgcaacaca gcgaagcgga catcgacaag cacctcgcgg tgtttgaaga aatcgcgccc
      301 ggtttggcta aggcgcaggc cgaacgcggc atggtgacgg tcgcagctgc tcactaggtg
      361 ttttgcgtcg ccggtcgcgt tgacctgcgg ccaaccgtca ttccgagcct gcccgacgca
      421 ggcgggcgaa gcgaggaatc tctcttggcg cggttttgac cgggattccg gggaaacttt
      481 tcggagaaga acgttgaccc aggaagtaaa caccgcggaa tcccacccgc atctcaaacg
      541 agttctcggc cagcgcgacc tggtgctgct gttcgtcgtc gccgttttca atctcaacgt
      601 cgtgccttcg attgcggcca acggcggcgt caccatttgg ctatggatca tttctttgat
      661 cctgtttttc tggccgcagg gaattgccgt catcgaactc gctcaccgtt atccaggcga
      721 aggcggcgtt tatctctggg ccaaagaagt tttcggcgac tttcacggat ttctatcggg
      781 ctggtgctac tggacgaaca acatgatgta cgtgcccact gtcctgctgt acttcgtcgg
      841 tgtctcggtc tttgcccttg gctccggcca cgaagcgctc gccgataaca aaatctttgc
      901 gctcgccgct tgcgtggttc tgctggctgt cttggtcatt ctgaatatcg ttggcctcgg
      961 cgtcggcaag tggatcaaca acgtcggcgg cataggcact tttttcgccg ccgcgctact
     1021 catggggctc ggcctgattg tttggactcg tttcggtacc accgtgacat ggtctgactt
     1081 tcagatcccc gcaaatccac gtttcgttct aaattccttc ggcgtcattt gcttcggcct
     1141 tgtcggtctg gaactcgcct ccgtcatggg cgacgaaatt caagatcctg ccaagacctt
     1201 gcccggtgct gtcgcttggg gcggcgtgct ttccggcgtc ctctatattg gcgcaaccct
     1261 tacgctcttg gtcgcagtca gcaagtccga catcagcgtc cttcagggga tcgtccaggc
     1321 cgtcacacgc atggctggcc gcgtcggcgt cctgtggatc gtcgctccct tcgccttttt
     1381 actcagcttg tctattgcag gaatcggctc tgcctggctc ggcggctccg cccgtatccc
     1441 gtttgtcgcc ggtctcgatt cctatatgcc gcgctggctc ggcagcatcc atgcgaaata
     1501 tcgcactccc tacgccgccc tgctcgttca cgccgtcgtc tccttgctgc tcgtgctcat
     1561 gaatttctgg ggctccggcg ttcaggaatc ttttcagcgc atgttgtcgc tcgccgtcgt
     1621 gctgcagctt gttccgtttc tctacatgtt cggcgctttg cttaagattg ccctcgacca
     1681 acgcttccag cccgcccgct acagcaaaag cactttgctg ctcgccggcg caagcggtct
     1741 attgaccact tcgctcggca tcggcctggc gttctttccc gcccagcaaa tttcttcgct
     1801 tgtatcctat gaaacatgga tggtgggtgg caccatcttc tttataggcg ccgcggcatt
     1861 tttctttttc gtctacggcc gccgcaaagt ggcttttaaa gcagcggctt aaagttttgg
     1921 cagtggggag gtttatatgt ccggcagtgc aacaacagcg ggagtccgca cctatcagaa
     1981 tttcgtcaac ggcaaatggg aaagcgctgc ctccggcaaa acgtttcccg tctatgatcc
     2041 ctccaccgag gaagtgatcg cccacgtcgc cgcggctgat gtggccgacg tcgatcgcgc
     2101 tgtcaaagcc gcacgtgctg ctttcgattc cggcccttgg ccgcaaacta ccgcgcaaga
     2161 ccgcggccgc atccttttca ggctcgcgga agaaattcgc cgaaactccg ctgcgctcgc
     2221 cgaactcgaa tgccgcaata ccggcaagcc catcgtcgaa gccgaatacg acatggccga
     2281 cgtctccacc tgtttcgaat attacggggg cctcgccaac aaagtcaccg gccacgtcaa
     2341 tccggttccc gccaacgccc tcagcttcac catgcgggag cccgtgggca tcgccggcca
     2401 aatcattccg tggaactatc cgctgctcat ggctgcctgg aaactcgcac ccgccatcgc
     2461 cgctggttgt tgctgtgtgc tgaagcccgc cgaacaaacg cctctcactg ctctcgaatt
     2521 cgcaaattat tttgaacagg ctggcctgcc gcctggcgta gtcaacatcg tcaacggttt
     2581 tggcgaaacc gctggcgccg cgctcgtcgc tcatcccggc gttgacaaaa tcgccttcac
     2641 cggcagcgct gccgtcggca aaatcatcgt caagtccgcc gccgatacac tcaagc
//
LOCUS       DASTPM010000014         6623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_865482, whole genome shotgun sequence.
ACCESSION   DASTPM010000014 DASTPM010000000
VERSION     DASTPM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865482"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFR42_00210"
     CDS             <1..654
                     /locus_tag="VFR42_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367600.1"
                     /translation="ESLLAMLALISWYSHSVTTWAANALDSALRGGPEASVPGQAIGF
                     SALVLWCLHPFTWIIAYFVVEGMVRFLAAVSTEQILPSCPLAIADWSYGKLTQRPSEG
                     DALHTPTAKQQVQSLAATVRQSAKTAGLTELPDELLESADGGDAILEIHSSRPKAEWT
                     PPRVVRVGVVYYRLEVASEGSRPRPFIYRLRRLPAGVPGRNVLLYEPPHAMESAKQS"
     gene            732..1613
                     /locus_tag="VFR42_00215"
     CDS             732..1613
                     /locus_tag="VFR42_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367601.1"
                     /translation="MAPLRPSPVWPAVRTAVLLLAAFVLPCSVVGQQAPSAKNARILL
                     LPRKMVSGDRTTLAVLDFGGRLTPNVTVAFSNGDHVTTNSTGRATFVAPLTQGVLYAT
                     IQGRAGRVQTEVIAASANKSATQIDRAPHFASLSDRFELQGHGFCGDADKNVVQINGR
                     PSLVLASSSESLAVLPSADLDPGPAEVSVSCNKGDAGWTRIVLLALSLEADTSPLKAG
                     EKRTLNVRISGTSERVSLEARNLAPEVAALDGGNTARSVSSGGAQNVAAFPLTGKQHG
                     NILVSIRLLPGVVRAQP"
     gene            complement(1584..2783)
                     /locus_tag="VFR42_00220"
     CDS             complement(1584..2783)
                     /locus_tag="VFR42_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU0367602.1"
                     /translation="MSGIVVVAALVALGLVVWLALQSLSAQKKTEGVESQMNELRRDL
                     QTVATAQAQAAGQISTLTSSVTTRLDAVSRSLTDGVAKSADISAQGQTAMREELKSTQ
                     SMMERIHKQLGEFQEVSRGLSNAQQSLESVLGGAKTRGILGEVTLDRLLEDSLPSSQY
                     GTQYRFASGEAVDAVIFLRDKKLLAVDSKFPLEAFRRIQTDGDEARRSFASAVKGHAD
                     SIAKKYIRPDENTLDLALMFVPSESVYYELLQTSDSKGQALDEYCREKRIVAVSPNTL
                     YAHLCVIAMGLRGMQIEENARRLANGLSGLQKQLGNFVDAFDKVGTHLKNAQNSYQEA
                     GKRLDRTELALEGMLTSGDASAGAQTELPLNSSSTNGSNATASSTADEKPDEPQQMLR
                     LSADNSR"
     gene            complement(2918..>6623)
                     /locus_tag="VFR42_00225"
     CDS             complement(2918..>6623)
                     /locus_tag="VFR42_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0367603.1"
                     /translation="NDEGRYGFVSLVPGKYQITVDAGTNFSVFEQDGLTITVGQEAEF
                     DPLLNLRGVATTTTVTAETTLIEENKTEVSNTVDQRRIDNLPINGRDYKNFTLTDSRT
                     TRDVSPTIGPAPNSGLNIGGARARSNMVSVDGADAVDNSVNGIRATVSQEAVQEFQMV
                     LSNYNAEYGRATGGVINIVTKSGGNELHGDAFVFGRNKSFQARNPFSGQIDPTSGELV
                     PVKQPYTRIQTGLTLGGPIKKDKTFFFGSYEYTQREETGFSSIGIDNFGMAPFNCGGC
                     GLDGLMLTPDQSAAVLQLLAGTPQQQALAQGYAVLMGSASSVALNRLDFGQVAAGLTS
                     SFLTPGPGAQFPIPVACPKGQTMNDSAGTGTAPVCSAAGVYVAPLPVSYVGLNSIRGN
                     YPIQEKTSLWSLKLDQNWNANNHSFIRVGVSPSLVTGLPSTSQNQVFGQNSGSRAGYN
                     QSRDLNVTFQHDTITSDSAVNQFRFQFARRGLHFGFSQLPGGSDIGVNIPGFAYFGRE
                     PYSTVDRIERRYEFTDTIAKTFGRHTLKFGVDFNLIQLRSAKAQIFELDFGGDVNFGG
                     IDASNFSSAFAGLPGATGLQAYGLGIPTTYIQGIGNSNQPFDNLPIGFFAQDSWKVNR
                     RLTLNYGVRYDVEISPLFAPATAINAAAEKALGVVEGIPRDYNNFAPRFGLAWDPTGS
                     GKTSIRLGYGLFYDHPLLAIAFNSVTADGGRSVQLLSAGGKPSACGLVTPDCATFGST
                     GTDSPANLNGSSIFQGVLNTGSADLAFSPLSLAYLPAQQRFDPLAPNSLFANQNYLTA
                     GFPLPILPFTLPVAANFKYGYAQQGNLTIERAFSNSWKVSVGYQYTRGIHLNRPVDIN
                     STDPQLLSQNAFNAAVSGLGVSNPVTVVVPSGAPNSCVNQGSGSIFLIAPGALGQGFA
                     APNCNAAAAVGFVGTPAYFNFFRPSGPNPSFAAATPGGYGTQVALAHLAGYPTGFGVP
                     VPFNSVDAQLSDASSWYNALTVNMQKRFSHGFELLSSYTWSHSIDTGTDLQSTLEPQD
                     SRFPNLEKANSVNDQRHRWVTSAVFMTGAPKSGDSAWRHFLGGFTFAPLIEVSSGRPF
                     NVITGTDTRLDLGASQARPSVVSSGTTSPFIPGAQFGVADVCLTNAGTPFTVPFFTPP
                     AGCDGSLGRNAFTGPGFFQIDMRLSKTIPLGERWSVDLIADGFNMLNRTNIAAVNQLC
                     DPTVGTVCLAGQPTAAYDARQFQFALKLHW"
BASE COUNT         1273 a         2045 c         1899 g         1406 t
ORIGIN      
        1 gaaagcctgc tggccatgct ggccttgatt tcctggtact cgcactccgt aaccacctgg
       61 gccgccaatg ccctcgactc cgccctgcgt ggcggaccgg aagccagcgt gccgggccag
      121 gccatcgggt tctcggctct ggtgttgtgg tgcctgcacc cgttcacctg gattatcgcc
      181 tatttcgtcg tcgagggaat ggtccgcttc ctggctgctg tttccactga acagatcctt
      241 ccctcctgcc cgctggccat cgccgactgg tcctatggca agttgacgca gcgccctagc
      301 gaaggcgacg cgcttcatac accgaccgcg aagcagcaag tgcaatcgct cgccgccacc
      361 gtcaggcagt ccgccaagac ggcaggcttg accgagctgc ccgacgagct tctcgaatca
      421 gccgacggcg gcgacgcaat cttggagatt cattcctcgc ggccgaaagc cgagtggaca
      481 ccgccgcggg tcgttcgtgt tggcgttgtt tactaccgcc tggaagtcgc ctccgaaggt
      541 tctcggccgc gaccgttcat ttaccggttg cgccgcctcc ccgccggcgt tcccggtcgt
      601 aatgtgctgc tttacgagcc gccacacgcc atggaatcgg ccaagcaatc gtgaactgtt
      661 tcaagctgac gctgtcccag ctgactcttg ttcctgttct cacctacaat gcccgtacaa
      721 tacttgctgc tatggcaccg ctccgcccca gccccgtttg gcccgccgtt cgcactgccg
      781 tgctgctcct cgcagcgttc gtgttgccgt gctcggtagt tgggcaacaa gcgccttccg
      841 cgaagaacgc tcgaattctc ttgttgccgc gaaaaatggt ttccggcgat cgcaccacac
      901 tcgcggtcct cgacttcggc ggccggctca cgccgaatgt caccgtggcg ttttctaatg
      961 gcgatcacgt gacgaccaac tccactggcc gcgccacttt tgtcgctccg ctcacgcagg
     1021 gcgttctgta tgccaccatt cagggccgag ccggccgcgt gcagaccgaa gttatcgccg
     1081 cttccgccaa caaaagtgcc acgcaaattg atcgcgcacc gcacttcgct tccttgagcg
     1141 atcgctttga attgcagggc cacggcttct gcggcgacgc cgacaaaaat gtcgtccaga
     1201 tcaacgggcg cccctctctc gttctcgcct cgtcttccga atcgctggct gtcttgcctt
     1261 ccgccgatct cgatccgggg cccgcggaag tcagcgtgtc ttgcaacaaa ggcgatgccg
     1321 gctggacgcg catcgtactc ctggcgctct ccctggaagc agatacttct ccgcttaagg
     1381 ctggcgagaa acgcacgctc aatgtgcgca tcagcggcac cagcgaacgc gtctcgctgg
     1441 aggcgcgcaa tcttgctcct gaagttgcgg cgctcgacgg cggaaacacc gcgcggtctg
     1501 tctcttccgg aggcgcgcag aatgtcgccg cctttccgct taccggaaaa cagcacggca
     1561 acattctcgt ttcgattcgc ttgttacctg gagttgtccg cgctcaaccg tagcatttgc
     1621 tggggttcgt ccggtttttc gtcggcagtt gaactggctg ttgcgttcga gccatttgtg
     1681 ctggaactgt tcagcggcag ttcggtttgc gcgccagccg acgcgtctcc gctggtgagc
     1741 atgccttcca aagcgagttc cgtgcggtcc agccgcttgc ccgcttcctg ataactgttc
     1801 tgtgcgttct tcagatgcgt tccgactttg tcgaacgcat cgacgaaatt tcccaactgt
     1861 ttctgcaaac cggaaagccc gttggccagg cggcgcgcat tttcttcaat ctgcatgccg
     1921 cgcaatccca ttgcgattac gcacaagtgc gcgtacagcg tgttcggcga aaccgccaca
     1981 attctcttct cgcggcagta ttcatcgagc gcctggccct tgctgtccga agtttgcagc
     2041 agttcgtaat acacgctctc cgacggcaca aacatcagcg ccaggtccaa agtattttcg
     2101 tcggggcgga tatatttttt ggcgatggag tcggcgtggc cttttaccgc ggacgcaaac
     2161 gaccgccgtg cttcatcgcc gtccgtctga atgcgccgga aagcttccag tgggaatttg
     2221 gaatccacgg ccagcagttt tttgtcgcgc aagaaaatca ccgcgtccac cgcttcgcca
     2281 ctcgcaaagc ggtactgcgt tccgtactgc gaagagggaa gggaatcctc cagtaaacga
     2341 tccagcgtca cctcacccaa aatcccacgc gtcttcgctc cgcccagcac ggattcaagc
     2401 gactgctgcg cattgctcag cccgcggctg acttcctgaa attcccccag ctgcttgtga
     2461 atcctctcca tcatgctttg cgtgcttttc aattcttcgc gcatagctgt ctggccctgc
     2521 gcggaaatat ccgccgactt cgccacaccg tcggtgagcg aacggctcac cgcgtcgagt
     2581 cgcgtggtca cgctggaagt cagagtggaa atctgtccag ccgcctgagc ctgcgcagtc
     2641 gccacggttt gcaggtcgcg ccgcagttcg ttcatttgcg attcgacgcc ttcggttttc
     2701 ttctgtgcgg ataggctctg caacgccagc caaaccacca gccccaacgc cactagcgcc
     2761 gccactacca cgattccaga cattaggttc tccccgatca tttcgggaaa cggtgactct
     2821 actgtagcgg gttgcgccgc agaggcaaac ccgcaaaaaa caaaacggcc cgctctttcg
     2881 agcaggccgc tgatcctagt tggctttcaa acccggctta ccaatgcagt ttcaacgcaa
     2941 actggaactg ccgcgcgtca tacgccgccg tcggctgccc ggcaagacac accgttccca
     3001 ccgtcgggtc gcacaactgg ttcaccgctg cgatattcgt gcggttcaac atgttgaacc
     3061 cgtccgcgat caggtccacg ctccaccgtt cacccagcgg gatggtcttc gacaaccgca
     3121 tatcaatctg gaagaatccc ggcccggtaa aggcatttcg tcccaggctt ccatcgcaac
     3181 ccgccggtgg ggtaaagaac ggcaccgtga agggcgttcc cgcattggtc aggcaaacat
     3241 cggccacgcc aaactgcgcg ccgggaatga acggcgaggt ggtgccgcta gaaacgacgg
     3301 agggccgcgc ctgggaagcg ccgaggtcga gacgagtatc ggttccggtg atgacattga
     3361 acgggcgtcc cgaggacact tcgatcagcg gcgcgaacgt gaatccaccg aggaaatgcc
     3421 gccaagcgct gtcaccgctc ttcggcgcac ctgtcatgaa caccgcgctg gtgacccagc
     3481 ggtgccgctg gtcgttcacg gaattcgcct tttccaaatt ggggaatcgg ctgtcctgcg
     3541 gctccagcgt ggactgcagg tccgtgccgg tgtcgattga atgcgaccac gtatagctgg
     3601 agagcagttc aaatccatgg gaaaagcgct tctgcatgtt tacggtcagc gcgttatacc
     3661 acgagctcgc gtccgacagc tgcgcgtcca cgctgttgaa cggcaccggc actccaaaac
     3721 cagttggata gcccgcgagg tgcgcgagcg cgacttgcgt cccgtagccg cccggggttg
     3781 ccgcggcaaa ggacggattc ggtcccgatg gccggaagaa gttgaagtac gccggagtac
     3841 cgacgaaacc cacggctgct gctgcattgc agttcggtgc cgcgaaaccc tggccgaggg
     3901 cacccggcgc aatcaggaaa atgctgccgc tcccttgatt cacgcacgaa ttgggtgcac
     3961 cagagggcac cactaccgtc acgggattgc tgacgcccaa ccccgaaacc gcggcattga
     4021 atgcgttctg cgacaacaat tgcggatcgg ttgaattgat atcgaccggg cgattgaggt
     4081 gaatgccgcg ggtgtattga tagccgacgc tgaccttcca tgaattcgaa aaggcgcgct
     4141 caatcgttaa gttgccctgt tgggcgtatc catacttgaa attggccgcc accggcaaag
     4201 tgaacggcaa aatcggcagc gggaatcctg ccgtcaaata attctgattc gcaaaaaggg
     4261 aattcggcgc cagcggatca aatcgctgtt gagctggcaa atacgccaga gacagcggcg
     4321 aaaacgccag gtctgccgat cctgtattca gcacgccctg aaaaatagag gagccgttca
     4381 aattagcggg gctgtccgtt cccgtgctgc caaatgtcgc acaatccggc gtcaccaggc
     4441 cgcatgccga aggtttcccg ccagcggata agagctgcac cgatcgccct ccgtccgccg
     4501 tgacggaatt gaacgcaatc gccagcagcg ggtggtcgta gaacagtccg tatccgagcc
     4561 gaatggaggt tttgccgctg ccggtcggat cccaggccag tccaaagcgt ggcgcaaagt
     4621 tgttgtagtc gcgtggaatg ccctctacaa cgcccagcgc tttctcggct gcggcgttta
     4681 tggcggtagc cggcgcgaac aacggtgaaa tttcaacgtc gtagcggacc ccatagttta
     4741 acgtgagcct gcggttgacc ttccaggaat cctgcgcgaa gaatccgatg ggaaggttgt
     4801 cgaagggctg gttggaattg ccgatgccct gaatgtaggt tgttggaatt cccaatccgt
     4861 aggcttgcaa gccggtggcg cccggcaatc ccgcaaaggc actactgaaa ttcgaggcgt
     4921 caattccgcc gaagttcaca tcgccgccga agtccagttc gaagatctgc gctttcgcgg
     4981 agcgcagctg aatcaggttg aagtccactc cgaacttcag cgtatgccgc ccgaaagttt
     5041 tcgcaatggt atcggtgaat tcatagcggc gctcgatgcg gtccaccgtg gagtacggct
     5101 cgcggccaaa atacgcaaag ccgggaatgt ttacgccgat gtcgctgccg ccgggcagct
     5161 gggaaaaccc gaagtgtaat ccgcgtcgcg cgaactgaaa gcggaactgg tttaccgcgg
     5221 aatcgctggt gatggtgtcg tgctggaagg tgacgttcaa atcgcgcgac tggttgtagc
     5281 ccgcgcgcga gccggaattc tgtccgaaca cctggttctg cgaagtcgaa ggaagtccag
     5341 tgaccagcga aggcgaaact ccgacccgga tgaaggaatg attgttagca ttccagttct
     5401 ggtcgagctt tagcgaccac aggctggttt tctcctgaat tgggtaattt ccgcgaatgg
     5461 aattcaggcc tacataggaa acgggcagcg gtgcgacata cacgcccgcg gcggagcaaa
     5521 caggcgctgt ccctgttcct gcgctgtcgt tcatcgtctg ccccttgggg caagcaaccg
     5581 gaatcggaaa ttgcgcgccg ggtcccggcg tcaagaagct gctggtcagg cccgccgcga
     5641 cttgtccaaa gtcgagccgg ttcagcgcca cgcttgatgc cgatcccata agcaccgcgt
     5701 agccttgggc cagcgcctgc tgttgcggag ttcctgccag gagctgcaac acggcggccg
     5761 actggtccgg tgtgagcatc aatccgtcca gcccgcatcc gccgcaatta aagggcgcca
     5821 tgccgaagtt gtcgatcccg atgctcgaaa atcccgtctc ttcgcgctgc gtgtattcat
     5881 acgatccgaa gaagaacgtt ttgtcttttt tgatcggccc gcccagcgtc aatccggtct
     5941 gaattctcgt gtagggctgc ttcacgggaa ccaattcgcc gctcgtcgga tcgatctgcc
     6001 ccgaaaacgg gttgcgagcc tggaacgatt tgttacgccc aaacacaaag gcgtcgccgt
     6061 ggagttcatt gccgccactc ttggtgacga tgttgatgac gccgccggtg gcacggccgt
     6121 attccgcgtt gtaattgctc agcaccattt gaaattcctg cacggcttct tgcgaaaccg
     6181 ttgcgcggat gccgttcacg gagttgtcca ccgcgtcggc gccgtccacg ctaaccatgt
     6241 tggagcgcgc gcgcgcgccg ccgatgttga ggccgctgtt gggcgctgga ccgatcgtcg
     6301 gggaaacgtc gcgagtggtg cgcgaatctg taagggtaaa attcttgtag tcgcgtccgt
     6361 tgatgggaag gttgtcaatg cggcgctgat cgaccgtatt ggatacttcc gttttatttt
     6421 cttcgatcag cgtggtttcc gcagtcacgg tggtcgtcgt cgcgacgccg cgcaggttga
     6481 gcagcggatc gaattccgct tcttgtccga cggttatggt cagaccgtct tgctcaaaca
     6541 cactgaagtt cgtgcccgcg tctacggtga tctggtattt gcccgggacc agcgatacga
     6601 acccgtaccg cccttcgtcg ttt
//
LOCUS       DASTPM010000015         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_58355, whole genome shotgun sequence.
ACCESSION   DASTPM010000015 DASTPM010000000
VERSION     DASTPM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58355"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFR42_00230"
     CDS             <1..786
                     /locus_tag="VFR42_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU0367604.1"
                     /translation="GWGLFTTIRISRGEAFAYERHWRRLEKDAALIRMPMPYTAAKIR
                     VHLHEVIRANNVSEGCARIYLIYNQVGFWRSDEPQPQVDLIIYSAPLPEYREPVRLGL
                     RAHGRHAAAPLAGVKSISWLPNVWAVAEAQKEGFDEVMLLNERGEVAECTAANIFAVK
                     GGKIYTPPLNSGCLEGVTRGILAEIAPEAGINVVQQALRPEDLYSSDEVFISSTNRNI
                     IGVGEIDGHKIAAAPGPITRQVEDLLVAHVTDYVTRRHATASR"
     gene            953..1858
                     /locus_tag="VFR42_00235"
     CDS             953..1858
                     /locus_tag="VFR42_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0367605.1"
                     /translation="MQVPQRPSAKSLLLAFALFFATLCTCLVAGTHFAIAYAHNEAVS
                     WDEFVHAFTLVYRNPAALVAGLPFALTLLAILLVHELGHFYACRHHHIHSSYPFFIPF
                     PTLIGTLGAFILIRSPIRNTRALFDVGASGPLVGFVVAVPALIYGIVHAKFIPGLSIP
                     GDNKFVLGAPLLLHVLAPIFHHGARAANLLLPPVGRAAWVGLFATSLNLLPAGQLDGG
                     HILRSVSQNAHRISSIFLPLLLAFFGFRYWPGWYVWAGLLVAIRFLRTLPIYHPEPLG
                     SRRQAAALFALLIFILSFMPAPFLE"
     gene            1904..>2290
                     /locus_tag="VFR42_00240"
     CDS             1904..>2290
                     /locus_tag="VFR42_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0367606.1"
                     /translation="MTCLTGTGTLFPPTNMAAILPPTTEASTQARASLLRTLRQRDLF
                     LLFIGSVIGSGVFLTPGLILRQLHGSVGYSFLVWIVGGILSLLGALTYAELAAANPQA
                     GGLYVFIRDGFGRMSAFLYGWCLFLVI"
BASE COUNT          358 a          713 c          659 g          560 t
ORIGIN      
        1 ggctgggggc tgtttaccac catccgcatt tcgcggggcg aagctttcgc ttacgagcgg
       61 cactggcggc ggctggaaaa agatgcggcg ctgatccgca tgccgatgcc gtatacggca
      121 gccaagattc gtgtgcactt gcacgaagtg atccgcgcga ataatgtcag cgaaggatgc
      181 gcgcgcattt atctgattta caaccaggtt ggattctggc gcagcgacga gccgcagccg
      241 caggtggacc tgatcattta ttccgcgccg ctgccggaat atcgcgaacc ggttcggctg
      301 ggactgcgcg cacatggccg ccacgctgcc gcaccgctgg ccggagtgaa gagtatctcc
      361 tggcttccta atgtgtgggc cgtcgctgag gcgcaaaagg aaggtttcga cgaagtgatg
      421 ctgctcaacg agcgtgggga agtggctgaa tgcacggcgg ccaacatttt tgcggtcaag
      481 ggcgggaaga tttatacgcc gccgctgaat tcgggctgcc tggaaggcgt aacccgcggc
      541 attctggcgg agattgctcc ggaggcgggc atcaacgtgg tgcagcaagc gctgcgaccc
      601 gaggatcttt attcttcgga cgaggttttt atctcttcga ccaatcgaaa catcattggg
      661 gtgggcgaaa tcgacggcca caagatcgcg gcggcacccg gaccgattac ccgccaggtg
      721 gaagacctgc tcgtcgcgca cgtaaccgat tacgtgacgc ggcgtcacgc aactgcctca
      781 cgctgacgct tggcgccttc aactggcgca acgctggcgc ctatgatgta cttgtcattg
      841 ctaattttca ggccgctctt atatcgtgca gaacacttct cccgaaagcg caaccacagt
      901 tgctccggta ccgcccgact ggcgggccga ggacgcttgg cgtgcgtacg ccatgcaggt
      961 gccgcaaagg ccatccgcga aatccctgct gctggctttt gcgctatttt tcgccaccct
     1021 gtgcacctgc ctggtggccg gcacgcattt tgcgattgcc tatgcccaca acgaagcggt
     1081 ttcctgggac gaattcgttc acgccttcac cctcgtatat cgcaacccgg cggccctggt
     1141 ggcaggattg cccttcgcgc taactctgct cgcgattttg ctggttcacg agctcggaca
     1201 tttttacgct tgccgtcatc accacattca ttccagctat ccgttcttta ttccttttcc
     1261 gacgctgatc ggcacgctcg gcgccttcat cctgattcgt tctccgattc gtaatacccg
     1321 cgcgctgttt gacgtcgggg cttcgggacc gctggtgggg tttgttgtag cggtgcccgc
     1381 gcttatctac ggaattgtgc acgctaagtt cattcccggg ctgtccattc cgggcgacaa
     1441 caagttcgtc ctgggggctc cgctgttgct gcatgtgctc gcgccgattt ttcatcatgg
     1501 cgcgcgggcc gcaaacctgt tgctccctcc ggtgggccgg gccgcgtggg tcggcctgtt
     1561 cgcgacgtcg ctgaatctcc ttccagccgg gcaactcgac ggcggccaca tcctgcgctc
     1621 ggtcagccag aacgcgcatc gtatttcgtc catctttcta ccgctgctcc tggcgttttt
     1681 cggcttccgc tattggcctg gttggtatgt ttgggccggg ttgctggttg cgatccgttt
     1741 tcttcgcacg ctgccgatct atcatccgga gccgttaggc tcgcgccgcc aagcggcggc
     1801 gcttttcgcg ctcttaatat tcatcctttc tttcatgccc gcgccgttcc tcgagtagct
     1861 gacatccctg gtttcgggct gcgcactgcc cgcaaacttc cggttgacct gcctgactgg
     1921 cacaggcact ctctttccac ctaccaatat ggccgcaatt cttcccccga ccaccgaagc
     1981 gtccacacaa gctcgcgctt ccctgctgcg caccctgcgc cagcgcgacc tgttcctgct
     2041 gttcatcggc tcggtcatcg gctccggagt tttccttacg cctggactga tcctgcgtca
     2101 gcttcatggc tcggtgggct actcattcct ggtatggatc gttggcggca ttctttccct
     2161 gcttggcgcg ctaacctacg ccgagctggc cgccgccaat ccccaggctg gcgggttgta
     2221 tgttttcatt cgcgatggtt tcggccgcat gtctgcgttt ctgtatggct ggtgcctgtt
     2281 cctggtgatt
//
LOCUS       DASTPM010000016         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1298176, whole genome shotgun sequence.
ACCESSION   DASTPM010000016 DASTPM010000000
VERSION     DASTPM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298176"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1074
                     /locus_tag="VFR42_00245"
     CDS             130..1074
                     /locus_tag="VFR42_00245"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEU0367607.1"
                     /translation="MPVQKTEKIWHNGKFIKWDDATIHVLSHVVSYGSAVFEGIRCYD
                     TKQGPAIFRLREHMQRLLNSAKIYRMELPFSVDEFGSAACELVRENKLNVCYVKPVVL
                     RGYGEVGVNPLNSPIECYMACWSWGAYLGPDALTKGVDIGVSSWTRIAPNTLPAMSKA
                     AANYMNSQLIRMEATFNGYAEGIALDSDGHVSEGSGMNVFLAHDGVLYTPPLATSILP
                     GITRDTVVKMAEDMGITVKETVIPREMLYIVDEVFFVGTAVEITPIRSVDHIQVGQGV
                     AGPITRKLQEEFFAITSGSKPDRHSWLTPVNAPVAAAR"
     gene            1293..1985
                     /locus_tag="VFR42_00250"
     CDS             1293..1985
                     /locus_tag="VFR42_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HEU0367608.1"
                     /translation="MYNTPQKLVAEFLGTFALVFFGAGAICAEQFLHGAGGAGLLGIA
                     LAHGLAIGVMICAIGHVSGGHFNPAVTMAFWVTKRLSTPEVFGYWISQLLGGIAAAFF
                     LKAILPREEAWLPVLGGTPDLARDFTRLPAIGLEAVATFFLVFVVFAAAVDEASSARP
                     YAGFCIGLTITLGILAIGPLTGAALNPARAFGPALASTHWANQGVYWVGPLTGGFFAG
                     LLHDALYGKKQL"
     gene            2174..3319
                     /locus_tag="VFR42_00255"
     CDS             2174..3319
                     /locus_tag="VFR42_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU0367609.1"
                     /translation="MALSIDDLLRRAVEQKSSDLHLKVGNHPYLRVDGLLTPLTDVPR
                     ITPEEMLSMAFSMMTNRQKQKFKETAELDMAYGVAGLGRFRVNVFQQRGNVGMVLRVI
                     PTKIRTADELNLPPVVNKICEEQRGLVLVTGTTGSGKSTTLAAMIDRINATRSDHLIT
                     IEDPIEFLHRDKRSFINQREVEVDTANFSTALRAALRQDPDVILVGEMRDLETISTAL
                     LAAETGHLVFSTLHTLDATETIQRIIAVFPPPEQKQIRLQLAATLKAVVSQRLVRRAD
                     DQGRVPAVEVMVATAYIRDCIINADKTRLIHDAIAAGTSQYGMQTFDQSLFDLYTKQL
                     ITLDEALLRASNADEFKLRIQGVRSAADAAREEMERQMADFERFARK"
BASE COUNT          716 a         1047 c         1006 g          774 t
ORIGIN      
        1 gccaaattgg ttctaggtgc tttcgtgttt cccggcacat tgagttgacc gctttgccga
       61 cacacgcgaa aatcaaacgg agtgggtggc aagatatctg gctgagtggc ttcgccgaaa
      121 ggaagcttta tgccggttca gaaaacggaa aaaatctggc acaacgggaa gttcatcaaa
      181 tgggatgacg cgacgatcca tgttctatcg catgtggtga gctacggctc cgcggtattt
      241 gaaggcattc gctgctacga caccaagcaa gggccggcga ttttccgttt gcgcgagcat
      301 atgcagcggc tgctcaactc cgcgaagatt tatcgcatgg agctgccctt ttcggtggac
      361 gaattcggca gcgcagcctg cgaactggtg cgcgaaaaca aattgaatgt gtgttacgtg
      421 aagcccgtgg tgcttcgcgg ttatggcgaa gtaggggtaa atccgctgaa ttcgccgatc
      481 gaatgctaca tggcgtgctg gtcgtgggga gcctacctag gaccggatgc gctgaccaaa
      541 ggcgtggata tcggcgtgag ttcgtggacg cgcatcgcgc cgaacacgtt gccggcgatg
      601 tcgaaagcgg ctgccaacta catgaactcg cagctgattc gcatggaggc gacgttcaac
      661 ggctatgccg aaggaattgc gctcgattcg gatggccatg tgagcgaagg ttccggtatg
      721 aacgtattcc tggcgcacga tggcgtgctc tatacgccgc cgctggccac cagcattctc
      781 cccggcatta cccgcgacac tgtggtgaaa atggcggaag acatgggcat caccgtgaag
      841 gagaccgtga ttccgcgcga aatgctgtac atcgtagacg aagttttctt tgtcggcacg
      901 gcggtggaga ttacgccgat tcgttcggtg gaccacattc aagttggcca gggcgtggcc
      961 ggaccgatca cccgcaagct gcaggaagaa ttttttgcaa tcaccagcgg cagcaagccc
     1021 gatcgccaca gctggctcac gccggtcaac gcgccggtgg cggcggcgcg ctaaactggt
     1081 tagaagctgg gatctcaaag aggggcgcaa gcgattgagc cctttcgtct ttcatccaag
     1141 aacttaacgc agagagcact gacgcgcaga gtccacggag ggttcgcaag cccgctcaat
     1201 gttccttaca ccgttaattc ctcacttcat tcccgcgcta gccgctgttt tgctagaatc
     1261 tgttccgttg ctccctcggt ctggagatgc cggtgtacaa cacgcctcag aagcttgtgg
     1321 ccgagtttct cggcaccttt gcgctggtgt tcttcggagc cggcgcgatc tgcgcggaac
     1381 agtttcttca cggagccgga ggagccggcc ttcttggcat tgccttggcg cacggcttgg
     1441 caatcggggt gatgatttgc gcgatcggcc atgtgtcggg aggacatttc aatccggccg
     1501 tcacgatggc tttttgggtc acgaagcgac tgagcacgcc cgaagtattt ggctactgga
     1561 tcagccagct cctgggtggg attgcggcgg catttttctt gaaggccatc cttccgcgcg
     1621 aggaggcctg gctgccggtt cttggcggga cgccggacct ggcgagagat ttcacgcggc
     1681 ttccggcgat aggtctggaa gcggtcgcaa ccttctttct ggttttcgtg gtatttgccg
     1741 ccgccgtgga tgaagccagc tccgcgcggc cttacgcagg attttgtatc ggtctcacga
     1801 ttacgctcgg cattctggcg attggccctc tcacgggagc agcgctcaat ccggctcgcg
     1861 cgttcggtcc agccctggcc agcactcact gggccaacca gggcgtgtac tgggtcggtc
     1921 cgctcacagg cgggttcttc gccggccttc ttcacgacgc cctctacggc aagaaacaac
     1981 tctagttgcg aatgcgtagg tttgccggct gtccatgcac ctgtcccacc gcgcagggta
     2041 actagaccgc tacccggcct gacgactcgc ttgctggcct gggatacggg tgctactttc
     2101 gcgataggtc attaccgcga aatttcgggc gttttgctgg ttattcaaaa actgagagct
     2161 gagaactgtc cccatggcac tgagcattga cgatcttctc cgccgcgccg tcgaacagaa
     2221 atcgtccgac ttgcacctaa aagtcggcaa ccatccctat cttcgcgtcg acggcctgct
     2281 gactcctctc actgacgtgc cccgcatcac gccggaagaa atgctgtcca tggccttcag
     2341 catgatgacc aaccggcaaa agcaaaagtt caaggaaacc gccgagctgg atatggctta
     2401 cggtgtagcc ggcctgggcc gcttccgggt gaacgtattt cagcagcgcg gcaacgtcgg
     2461 tatggttctc cgcgttattc ccaccaagat tcgcactgcc gacgaactga acttgccgcc
     2521 ggtggtcaac aagatttgcg aagagcaacg cggacttgta cttgttacgg gaaccaccgg
     2581 ttccggtaag tccaccactc tcgccgcgat gatcgaccgc atcaacgcta cgcgctccga
     2641 tcacttgatc accatcgaag accccattga gtttctgcac cgcgacaaac gcagtttcat
     2701 caaccagcgc gaagtcgaag tggacacggc gaacttctcc acggcgttgc gcgctgcgtt
     2761 gcgtcaggac cccgacgtga ttctggtcgg cgaaatgcgc gacctggaaa ccatttccac
     2821 agctttgctg gccgcggaaa ccggtcacct ggttttctcc accttgcaca cattggacgc
     2881 tacggaaacc atacagcgca ttatcgcggt ctttcctccg cccgaacaaa aacagattcg
     2941 cttgcagctt gccgccactt tgaaagcggt cgtgtcgcag cgcctggtgc gccgcgccga
     3001 cgaccaaggc cgcgtccccg ccgtcgaagt tatggtcgcc accgcgtaca ttcgcgactg
     3061 catcatcaat gccgataaaa cacgcttgat tcacgatgcc attgccgccg gcacctcgca
     3121 gtacggtatg cagaccttcg atcagtcgtt gttcgatctc tacaccaagc aactcatcac
     3181 gcttgacgaa gccctgctgc gcgcgtcgaa tgccgacgaa ttcaagctgc gcatccaggg
     3241 tgtccgctcc gccgccgacg ccgcgcgcga agagatggaa cgccagatgg ccgacttcga
     3301 acgcttcgcc aggaaatgac ccaaatggcc aatggcctcc agagttgtgg ccgccatttt
     3361 ccgaagaaaa gctgtgccaa aaccagcatc ggtcacacct atccctgatt ctttgagccc
     3421 gttccgctcc cagccgacca gatcgtgact ggaaggttcg ccctgccttt tcttgcgctc
     3481 gtatggccag ctatctttac aggtaacaca tcgtttctca tcgttccttt cggctacgtc
     3541 gca
//
LOCUS       DASTPM010000017         3882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1269311, whole genome shotgun sequence.
ACCESSION   DASTPM010000017 DASTPM010000000
VERSION     DASTPM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269311"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..982
                     /locus_tag="VFR42_00260"
     CDS             212..982
                     /locus_tag="VFR42_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0367610.1"
                     /translation="MSDGRDIVKAESGAGNFGECLVEGSAEDKKRGRKIKRRALAVSI
                     ALQSLGLTALVIAPMLAKPAELVVTTTMPIPPYSSRPAPRRAATPDPGRTTHPCIVCT
                     NLPVAPITPTFGKIATTNTGDPDPLSIGTGTTDSGNGLLSMFDPRKQPKRPDDTPHEV
                     GRIHEPSIDPAMLMRRVEPIYPALARQIRKSGRVELRALIATDGTIQSLQVVGGDPLF
                     VRSALDAVGQWRYKPTLLNGQPVEIDTFITVIYTLNQQ"
     gene            complement(1190..1753)
                     /locus_tag="VFR42_00265"
     CDS             complement(1190..1753)
                     /locus_tag="VFR42_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367611.1"
                     /translation="MKLKVVVCLLAFASLFAAGVSAQDVQKFDLFAGYSYVRANPGTS
                     GVDGFNLHGGSASIAYNATSWLSGVADFGGYHSGNILGTGVDGTLSTYLFGPRVSYRH
                     HSHFTPFGQVLFGVAHIGGSDGLVFSTSNNSFAMALGGGVDVNVSHRFAIRPVQVDYL
                     LTRFNEFGNGAQNQNNLRVSTGVVFHF"
     gene            complement(1981..>3882)
                     /locus_tag="VFR42_00270"
     CDS             complement(1981..>3882)
                     /locus_tag="VFR42_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU0367612.1"
                     /translation="VGISWLPLYHDMGLIGAWLTLLHFGTPLSVMSPLAFLTRPERWL
                     WAFHKHRGTITAAPNFAYELCVRKIADKDIEGLDLSSMRAALNGAEPVNPETLGRFEQ
                     RFAKYGFRREAQLPVYGLAEASLAVTFPPLNRGPRIDLINREIFTAQGRAEPATGDPA
                     AIAFVSSGMPIPRHEVRIVDDSGNDVPDRTEGFLWFRGPSATTGYFNNPAATHTLFPE
                     GEPSHGEFAWVNSGDRAYRADGEIFVTGRVKDIIIKGGRNLYPHEVEELAGRADGIRK
                     GCIVAFGLKDLGTGTEKLVVVAESRESDPSRRSAIAAAVTYEVSAGLGLPPDRVELIP
                     PGSIPKTSSGKLRREETRQLYIAGTLSAKKAPAWLQIARLGTSGTAKNIFRNTVRGLK
                     RGLEILYGIYFILLFAVWIVPACAGVLFVKDHRAAGRYTSAVVKLLFFLAGIRVRVVG
                     KEHMNAPGAKIYASNHTSFFDVLPLMGALGVPYRFVAKHEVHAMPFIGTFLRKMKHLS
                     FERSDSNSRREAVQEMESTLRDGESVFVFPEGTFVPEEGVRPFQLGAFHTAVSTGIPI
                     VPVSLAGTRRFLRDGTLLPRPTTVTITLSPPVAPSVAAANSSDWHQVVRLRDTVREQI
                     ARFSGEPLI"
BASE COUNT          865 a          990 c         1303 g          724 t
ORIGIN      
        1 aacctcacat ccctgcgagc gccgcgccgg ccactgcatg gcaacctgca ggagccggtg
       61 cggcaaaagt tgccaactcg cggggctctc tcagggataa gcaaaacaat ttgatcggct
      121 gaagacccct atagaacagg ggacggaagc tgtgtgtctg caagagagtt tgcaaacgcc
      181 agatcctgaa ggactgagaa gggggtgtgc gatgtctgac ggaagagaca ttgtgaaggc
      241 agagtcagga gcgggaaatt ttggcgagtg tctggtcgag ggcagcgcag aagacaagaa
      301 acgcgggaga aagatcaagc ggcgcgcgct tgcggtttca attgcgctgc agagcctggg
      361 tctgacggcg ctggtgattg cgccgatgtt ggcgaagccg gcggaactgg tggtaactac
      421 tacgatgccg attccccctt atagttcgcg acctgcgcct cgtcgcgcgg ctacgccgga
      481 tccggggcgc acaacacatc cctgtatcgt ctgtacgaat ttgcccgttg cgccgattac
      541 cccaaccttc ggtaagatcg cgacaacgaa caccggtgat cccgacccat tatcgatcgg
      601 tacgggcacg acggattctg gcaacgggct gttaagcatg ttcgatccgc gcaaacagcc
      661 taagcggccc gatgacactc cgcacgaagt cggacggatt catgagccga gcattgatcc
      721 ggctatgctg atgcgccgcg tggagccgat ctatccggcg ctggcgaggc agattcggaa
      781 gagtggacga gtggagctgc gcgctctgat tgcgacggat ggcacgattc agtcgctcca
      841 agtagtgggc ggtgaccctc tgttcgtgcg gtcggcgctg gatgcggtgg ggcagtggag
      901 atataagccg acgctgttga acgggcagcc ggtggagatc gatacgttta ttaccgtgat
      961 ttacacgcta aaccagcagt aaaaagtggc ttggcgccgg ttcctcccag gaaccgttgg
     1021 gtgcgcgggc tgcatgcggt ctcagaacag cgagaacccg acggacggtg caagggctca
     1081 cgtctgcccc gtgggccctt ttttctgcct tgcaccggtc accgaaatga agcgggccgc
     1141 cagttgaaac ggcgcgagtc acttcggaat ccttcacgac cgaaaactgc tagaagtgga
     1201 aaacgacgcc ggtggaaacg cgcagattgt tctgattctg cgcaccgttg ccgaattcgt
     1261 tgaaacgggt gagcaggtag tccacctgca cgggacggat ggcgaagcga tgcgagacat
     1321 tgacgtccac gccgccgccg agagccattg cgaaagaatt gttggaggtg gagaaaacga
     1381 ggccgtcgct gccgccgatg tgcgctacgc cgaagagaac ttgtccgaac ggtgtgaagt
     1441 gagaatggtg gcgatacgaa acgcgcgggc cgaatagata tgtggagagc gtgccgtcca
     1501 cgccggtacc gagaatattt ccgctgtgat agccaccaaa gtcagcgacg ccactgagcc
     1561 agctggtggc gttgtaagcg atggaagcac tgccgccgtg gagattgaac ccatccacgc
     1621 cggaagttcc cgggttggcg cggacgtagg aatatccggc gaagagatcg aacttctgca
     1681 catcctgcgc ggatacgccg gcggcgaaaa gtgaagcgaa tgcgagcaag cacactacga
     1741 ccttcaactt catgagtcct ccaaatgggt gtatttgttg cgcgagcgcg cgccagaaat
     1801 gcgcggctac acgtgcgcgg cgcgcttcag gcagggaagc ggcgaaacgc aattatttaa
     1861 ttgtgttgtc ataagagatg cgttgcggca gggagaagtt gcaagaggtt gcgtgggtaa
     1921 cgtttttctt tacagagatt tatgcggaga acgacggaag attttttctt agattatttc
     1981 tcaaatcaag ggctcgccgg agaagcgagc gatctgttcg cggactgtgt cgcgcaggcg
     2041 gaccacctgg tgccagtcgg aggaattggc agcagcaacc gaaggagcga ccggaggcga
     2101 aagcgtgatg gtgactgtcg tggggcgggg aagaagggtg ccgtcacgga gaaaacggcg
     2161 cgtgccagcc agagaaactg gaacgatagg aatgccagtg gagaccgcgg tgtgaaacgc
     2221 gcccagctga aacgggcgga caccttcttc ggggacaaac gtgccttcgg gaaaaacgaa
     2281 aacggattcg ccgtcgcgca gagtggattc catttcctgg acagcctcgc ggcgcgaatt
     2341 ggagtcgctt cgttcgaacg aaagatgctt cattttgcgg aggaacgtgc cgatgaacgg
     2401 catggcgtgg acctcatgtt tggcgacgaa gcgataggga acgcccagcg cgcccatgag
     2461 cgggaggacg tcgaagaaac tggtgtgatt cgaagcgtag atttttgcgc cgggcgcgtt
     2521 catgtgttcc ttgccgacga cgcggacgcg aatgccggcg aggaagaaaa ggagtttcac
     2581 gacggcggag gtatagcgac cagcggcgcg atggtccttg acgaaaagaa cgcctgcgca
     2641 ggcgggaaca atccagacag caaatagaag aatgaagtag atgccgtaga gaatctccag
     2701 gccgcgcttc aagccgcgaa ccgtgttgcg aaaaatgttt ttggccgtgc cggacgtgcc
     2761 gaggcgcgcg atttgcagcc aggccggagc tttcttggcg ctcaatgttc ccgctatgta
     2821 gagctggcgt gtttcttcgc ggcgaagctt gccgctggaa gttttgggaa tgctacccgg
     2881 aggaatgagt tcgacgcgat cgggcgggag gccgagtccc gcggagactt cgtaggtaac
     2941 ggcggcggcg atggctgaac gacgcgaggg atcggattcg cgggattccg cgaccacgac
     3001 aagtttttcg gtgcccgtgc ccaaatcttt caaaccgaac gcgacgatgc aacccttacg
     3061 aatgccgtcg gcgcggcctg ctagctcttc gacttcgtgt ggatagagat tgcggccgcc
     3121 cttgatgatg atgtctttca cgcgaccggt gacgaagatt tcgccgtcgg cgcgataggc
     3181 gcgatcgcct gaattaaccc acgcaaattc tccgtgcgac ggctctcctt cggggaaaag
     3241 agtgtgcgtc gcggcgggat tgttgaagta acccgtggtg gcagaggggc cgcgaaacca
     3301 caggaagcct tcggtgcggt ccgggacgtc gttgccggaa tcgtcgacga tgcggacttc
     3361 gtgacgcggg atgggcatgc cggaagaaac aaacgcgatg gcggcagggt cgcccgttgc
     3421 gggttcggcg cgaccttgcg cagtgaaaat ttcccgattg atgagatcga tgcgcgggcc
     3481 gcgattgagg ggtggaaacg tgacggcgag agaggcttca gctaggccat aaaccgggag
     3541 ctgggcctcg cggcggaaac catatttggc gaagcgttgt tcgaagcgcc cgagcgtttc
     3601 cggatttaca ggttcggcgc catttagcgc ggcacgcatg ctgctgaggt cgaggccttc
     3661 aatgtccttg tcggcgattt tgcggacgca aagctcgtaa gcgaaattag gagcggcggt
     3721 gatggtgccg cgatgtttgt gaaaggccca gagccagcgt tcaggacgcg tgagaaaagc
     3781 gagcggcgac atgacggaaa gcggcgtgcc gaaatgcagc aatgtgagcc atgcgccgat
     3841 gagtcccatg tcgtgataga gcggcagcca gctgatgccc ac
//
LOCUS       DASTPM010000018         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_317943, whole genome shotgun sequence.
ACCESSION   DASTPM010000018 DASTPM010000000
VERSION     DASTPM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317943"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VFR42_00275"
     CDS             <1..652
                     /locus_tag="VFR42_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0367613.1"
                     /translation="TAQVAAQLGLTTFVHKQNLGYGRNQKTCYREALRRGADIVIMLH
                     PDYQYSPNLIVSMAAMIAYGEYDVALGSRILGVGALKGGMPLYKYISNRFLTLFENFL
                     LKYKISEYHTGYRAFSRTVLDALPLEANSDDFVFDNQMLAQIIFFGYRMGEVSCPTRY
                     FPEASSINFWRSCKYGLGVLATSMQFRLQKWGLANFRIFSSEGHKLLPDYYTMVKE"
     gene            736..2559
                     /locus_tag="VFR42_00280"
     CDS             736..2559
                     /locus_tag="VFR42_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0367614.1"
                     /translation="MIALGLIALTLVAYWPTFSNDFVNYDDPGYVVQNHFVQTGLNAS
                     SIAWAWRATIMANWHPLTWMSHMVDVRLFRLNPAGHHASSLLLHILNVVLLFFLLLKA
                     TGYLWRSGLVAAIFAVHPLNVECVAWISERKSLLCTAFLFFALLAYGWYVRQPGVTRY
                     LLVALLFALGLASKPMIITLPFALLLLDYWPLQRLPVPDSAENRPLFWKQLSGLALEK
                     VPLLLLSAGSAYITVFSQAKTNTIAANAFLSVPVRIANALWAYLMYIAKGIWPIHLAI
                     FYPHPESTIGWWRPALALLLIVLFSLFCLRHLGRRYLSVGWFWYLGCLVPVIGVIQVG
                     RQAMADRYAYATLPGIVVVVVWWVADYLAARRMKTESAGVCAVLVLIFFSVLTSRQTA
                     YWKDSFTLFQHALAVTPNNFIAETNLGEAFMQIGRPDLAYDHFLRATQEKPRFGVAQY
                     NLGTVLAGQGRRKEAREAFQMAIRYGQEGPDVAQAYHNLGIVMLDENELSEARAMFSE
                     ALRLVPARQSSYLARGMASFRLNDYRAAESDFIAATSLSPDPSAYFWIGRCREKLGDL
                     AGATEAYRKALALRPEMKDAKDRLDALLSGRPLPFLSQGSN"
     gene            complement(2571..>4259)
                     /gene="aceE"
                     /locus_tag="VFR42_00285"
     CDS             complement(2571..>4259)
                     /gene="aceE"
                     /locus_tag="VFR42_00285"
                     /inference="COORDINATES: protein motif:HMM:NF036501.4"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0367615.1"
                     /translation="VSSGAYVRQHFFGNDERLLKLVEPLPDEALRRLRLGGHDPRKVY
                     AAYKAAVEHKGGPTVILARTIKGYGLGEAGEGKNVTHQQKKLNEEELREFRSRFGIPL
                     NDEDCSTVPFYRPSEDSIEIQYLRARRESLGGYTPARSVRSTPIVADHDELFKEFTEG
                     SEDREVSTTMAFVAMLRKMLKDREIGKLIVPIVPDEARTFGMESLFRTVGIYASGGQK
                     YEPVDMNTLLLYREAKDGQILEEGITEAGSMASFIAAGSAYASHGINTIPFFIYYSMF
                     GFQRIGDFVWAAADMRTRGFLLGGTAGRTTLSGEGLQHQDGNSHILALPVPNLKAYDP
                     AYAYEIAVIIQDGIRRMYKDGENWFYYITVMNEPYAMPPMPAGVREGILKGIYKFRAS
                     TNKKSKLKAQLFGSGAILNEALRAQEILEQKYGVAADVWSVTSYKELYVDAIDAERWN
                     RLNPGAKPKVPYVSQCLADAPGVLVAASDYLKTLPNMIAKWMPRRLAALGTDGFGRSE
                     GRTALRDFFEVDAKFIALATLHELSADGKIDAKTVEKAAEELGISRDKPNPVVS"
BASE COUNT          795 a         1279 c         1179 g         1006 t
ORIGIN      
        1 gaccgcgcag gtcgcggcgc agctcggact gaccaccttc gttcacaaac aaaacctggg
       61 gtacgggcgg aaccagaaga cctgctatcg cgaagcgctt cgccgcggtg cggacatcgt
      121 gatcatgttg catcctgatt atcagtattc gccaaactta attgtttcta tggctgcgat
      181 gatcgcttat ggcgaatacg acgttgcgct cggctcgcgc atcctgggcg tcggcgcgct
      241 caagggcgga atgccgctgt acaaatacat ttcgaaccgg ttcctcaccc tgtttgagaa
      301 cttcttgctg aaatacaaga tttccgaata tcacacggga tatcgggcgt tttctcgcac
      361 ggttctggat gcactgccac tcgaagccaa tagcgacgac tttgttttcg ataatcagat
      421 gctggcgcag attattttct ttggatatcg catgggagaa gtttcctgcc cgacgcgcta
      481 ttttccggaa gcatccagca tcaatttctg gcgcagctgc aagtacggcc tgggcgttct
      541 tgctacatcg atgcaattcc gcctgcagaa atgggggttg gcaaacttcc gcatctttag
      601 cagcgaaggc cacaagctgc tgcccgacta ttacacgatg gtgaaggagt gaccgctccc
      661 gaaatctctc cagcgtcttc aactgacgca acgaactctt cagacggcgc ggcgcgattt
      721 cgctcgcgcc cttggttgat agcgctcggt ctgatcgcgc tcacgctggt cgcttactgg
      781 cccacattct ccaacgattt cgtcaattac gacgaccccg gctatgtcgt tcagaaccat
      841 ttcgtgcaaa ccggtctcaa tgcgtccagc atcgcgtggg cgtggcgcgc caccatcatg
      901 gccaactggc acccgctgac ctggatgtcg cacatggtgg acgtgcgcct gtttcggctc
      961 aatcctgctg gccatcacgc atccagtttg cttctccaca tcctaaacgt tgtgctgctg
     1021 ttctttcttc tcctgaaagc cactggttac ctgtggcgta gcggcctggt tgccgcaatt
     1081 tttgccgttc accctttgaa cgtcgagtgc gtcgcctgga tttctgaacg caaaagctta
     1141 ctctgcacgg cgttcctgtt ttttgcgctg ctggcgtacg ggtggtatgt ccgccagccc
     1201 ggcgttacaa ggtacctgct ggtagccttg ctcttcgcgt tggggctcgc gtccaagccc
     1261 atgatcatca cgctgccttt tgcactcttg ctcctcgact actggccgct gcaaaggcta
     1321 ccggttccgg atagcgccga gaaccgcccg cttttctgga agcagctctc aggcctcgct
     1381 ctcgagaagg ttcctctact tctgctttct gccggcagcg cctacatcac ggttttttcc
     1441 caggccaaga ccaataccat cgcggcgaac gcattccttt ccgtgcctgt ccgcattgca
     1501 aacgcattgt gggcttacct gatgtacatt gcgaaaggca tttggccaat tcatctggca
     1561 atcttttatc cgcaccctga aagcaccatt ggttggtgga gaccagcgct ggcactcctg
     1621 ctcatcgtac ttttttccct cttctgcctg cgccacctgg ggcggcggta tctatcggtg
     1681 ggctggtttt ggtatctcgg ctgcctggta ccggtaatcg gggtcatcca ggtcggccgc
     1741 caagcgatgg ctgatcggta cgcgtacgcg acgctgcctg gcatcgtggt agttgtggtt
     1801 tggtgggtcg ccgactatct ggccgcgcgg cgaatgaaga ccgaatccgc tggtgtatgc
     1861 gcagttctcg ttctgatctt cttttccgtc ctcacttcgc ggcaaaccgc ctattggaaa
     1921 gacagcttca cgctgtttca gcatgctctt gccgtcacac cgaacaactt catcgccgaa
     1981 accaatttgg gcgaagcctt catgcagatc ggtcggccag atctggccta cgatcacttc
     2041 cttcgcgcca cgcaagagaa gccacgattt ggagttgcgc aatacaacct gggaacggtt
     2101 ctggccggtc agggccggcg caaagaagcc cgcgaggcct ttcagatggc gattcgctat
     2161 ggccaggaag gtcccgacgt ggcgcaagcc tatcacaatc ttggcattgt aatgctcgac
     2221 gagaacgagt tgagcgaggc gcgagcgatg ttttccgagg cgcttcgcct tgtgcctgcc
     2281 aggcaatcca gctacttggc ccgtgggatg gccagcttcc gcttgaacga ttatcgcgcg
     2341 gccgaatctg acttcattgc ggccacgtcc ctttcgcccg atcccagtgc ttatttctgg
     2401 ataggacggt gccgcgaaaa gctgggagac ctcgcgggag cgacggaagc gtaccgcaag
     2461 gctctggcgt tacgaccgga gatgaaagac gccaaggatc gcttggacgc gttgctctcc
     2521 gggcgcccgc tcccctttct ttcgcaaggc agcaactgac agcttcgcgc tcaggaaacc
     2581 acggggtttg gcttgtctct tgagatgccc aactcttcgg cggctttctc gacggtcttg
     2641 gcgtcgattt ttccgtccgc gctgagttcg tgtagtgtcg ccagggcgat gaacttggcg
     2701 tctacttcga agaaatcgcg caacgccgtg cggccttcgc tgcggccgaa tccgtcggtg
     2761 ccgagcgccg ccagtctgcg cggcatccac ttggcaatca tgttgggcaa ggtcttcaag
     2821 tagtcggagg ccgccaccag cacgcccggc gcatccgcca ggcattggga aacatacggg
     2881 acctttggct tcgcgccggg gtttaggcgg ttccaccgct cggcgtcaat cgcgtcgacg
     2941 taaagttcct tatagctggt tacgctccaa acatccgccg caacgccata cttctgctcc
     3001 agaatttcct gggcccgcaa tgcttcgtta agaattgcac cgctgccgaa aagctgcgcc
     3061 ttgagcttgg acttcttgtt cgtcgaagcc cggaacttgt agataccttt taggatgcct
     3121 tcgcgcactc ccgccggcat cggcggcatc gcataaggtt cgttcatcac ggtgatgtag
     3181 tagaaccagt tttcgccgtc cttgtacatg cggcgaatgc catcctggat gatgaccgcg
     3241 atttcgtagg cataagctgg atcgtaggcc ttcaggttgg gcacaggcag cgcgagaatg
     3301 tggctgttgc cgtcctggtg ctgcaatcct tcgccggaaa gggtggtacg tcccgccgtc
     3361 ccgcccagca agaagccgcg cgtgcgcata tcagctgcag cccacacaaa atcgccgata
     3421 cgctgaaagc cgaacatcga gtagtaaata aaaaatggaa tcgtgttgat gccgtgcgag
     3481 gcataagcgc tgcccgccgc gataaacgac gccatcgaac cggcttcggt gattccctct
     3541 tccaggatct gcccatcctt ggcttcgcgg tagagcagca gggtgttcat gtccacgggt
     3601 tcatacttct gcccgccgct ggcgtagatg cctactgtgc ggaacaggga ctccatgccg
     3661 aaggtgcgcg cttcgtcagg aacgatgggc acaatcagct tgccgatttc ccgatccttg
     3721 agcattttgc gcagcatggc gacgaaggcc atggtagtgg aaacttcgcg gtcctcgctg
     3781 ccttccgtaa attccttgaa caactcatcg tgatcggcaa caatcggcgt cgaccgtacg
     3841 ctacgcgccg gtgtatagcc gccaagcgat tcgcgacgcg ctcgcaggta ttgaatctcg
     3901 atgctgtctt ccgatgggcg gtaaaacggc accgtcgaac aatcttcgtc atttagagga
     3961 atgccgaagc gagagcggaa ttcgcgcagc tcttcttcgt tcagcttctt ctgttggtgc
     4021 gtgacgttct tgccttcgcc cgcttcgccc agaccgtagc ccttgatcgt gcgcgccaga
     4081 ataactgtcg ggccaccctt gtgttccacg gcggctttgt atgccgcgta caccttgcgc
     4141 gggtcgtgtc cgccaagccg caaacgccgc agcgcctcgt ctggcagcgg ttccaccagc
     4201 ttcagcagac gctcgtcgtt tccgaagaag tgctgccgca cgtaagcgcc cgacgagac
//
LOCUS       DASTPM010000019         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_577411, whole genome shotgun sequence.
ACCESSION   DASTPM010000019 DASTPM010000000
VERSION     DASTPM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577411"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1868
                     /locus_tag="VFR42_00290"
     CDS             474..1868
                     /locus_tag="VFR42_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0367616.1"
                     /translation="MPITQPPEAGASKLAAKLGLFDATMIVMGGAIGSGIFVNPAVVA
                     RHVGSPVLILGAWLVGGVLALMGGMIYAELGVILPKAGGSYAYLHDCFHPILGFLYGW
                     FSLLVINAGGTAAVSLVFAKYVSVLLPVAIPEKVVAAGTILVLAAINCLGVRVGSNVQ
                     SALMILRAAGILVLIASGIGWCWTNSAATSLAWRPILDRPVSVDLGVAFGSAMIPVFF
                     AYGGWQTANFLAAEMREPRKNLPRALVLGVVGVVAFYVGVTFACLAVLGPAGLARTST
                     PASDVMQRVLGSKGAAFMAVGIAISTLGFLSQSVLTYPRVFFAMAADGVFPAGVARVN
                     ARTQVPVVAILLAGLLTVAVVLAGNYDAILVYVESMDGLFFGLSALALLVLRRRAAAA
                     GMQAVFRAPGHPWTTLIFVAAYWVVVATSFYKYRANAALVMAILLAGVPLYFYWSRPR
                     KAPASEGGRYVGEE"
     gene            1865..2944
                     /locus_tag="VFR42_00295"
     CDS             1865..2944
                     /locus_tag="VFR42_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0367617.1"
                     /translation="MSYRRTQARTAYIEWAKLSASAKFNLAASGVKGLPLRELPLPWD
                     ELELSAPGGYGYPPLLERLAARYGVSTKNVVTAIGTSMANYLAMSAVLEPGDEVVIED
                     PAYDPMVEVGHYLGAVVKRIPRRAENKFQIDVDELKEIVSGKTRLIVLTNLHNPSGVL
                     LEAEALREIGEVARSVGALVMVDEVYLELLFDRPLRPAFHLGSHFIVTSSLTKAYGLS
                     GLRCGWILAEPELAKRMWRQNDLFGNIPAHITELLSVVALDHLEAIRARAKDLLARNW
                     NLLDAFLAEHPELDVVRPQGGTIVFPRVPNGDGDAFCKHLSGKYETSAVPGSFFSMPD
                     RIRIGICNDPVEVEEGLRRVGRLLL"
BASE COUNT          557 a          735 c         1114 g          707 t
ORIGIN      
        1 tgtggacggt gaacgagagt gctcgggaag cttggtcgaa ccgatggcgc ggcggcacga
       61 acctgcctga tcttgctgga taattgttaa cttccatgtc atacttttag atgtattata
      121 gtagtacctc gattcgcgcc attcgccttc gaagcttccg cgttcccgtc gcacctcttg
      181 cggcacagca gggcgacaca agcggcaagc tctttctttt catgcagttt cgcggtcctc
      241 ggcaacggcc gaaagctcat ctcccctatt ttcagtcgtt tacgcattct taggccaaaa
      301 acaccggggg tggcatggcg aggacctccg ttctgcccac ccattttggc ccatcttggg
      361 aaccaggcgt cctcacgaac aaggcacagc gatgctgcta gaatgccgcc gctgtatcag
      421 caaactccgc cctctgaagt gggcggctac agggtgtgtg catcgtgagc agtgtgccca
      481 taacgcagcc cccggaagcc ggcgcctcta agctggcggc gaagctgggg ctctttgacg
      541 ccaccatgat cgtgatggga ggggcgatcg ggtcggggat ttttgtgaat ccggcggtcg
      601 tagcgcgcca tgtcggttcg ccggtgttga ttttgggtgc ttggctggtg ggcggagttc
      661 tggccctaat gggcgggatg atctacgcgg agctgggcgt gatcttgccg aaagcgggcg
      721 ggtcgtacgc ttatctgcac gactgttttc atccgatcct gggatttttg tatggatggt
      781 tttcgctgct ggtgatcaac gcgggcggaa cggcggcggt gtcgctggtg ttcgcaaagt
      841 atgtttcggt gctgttgcca gtggcgattc cggaaaaagt agtagcagcc ggcacgatcc
      901 tggttctagc ggcaatcaat tgcctgggcg tgcgagtcgg gagcaacgtg cagtcagcgc
      961 tgatgatttt gcgggcagcg ggaattctgg ttttgattgc cagcggaatt ggctggtgct
     1021 ggacgaactc cgcggcgacc agtttggcgt ggagaccgat tctggacagg ccggtgtcgg
     1081 tggatttggg agtggcattt ggttcggcaa tgattccggt attttttgcg tatggcggat
     1141 ggcagacggc gaattttctg gccgcggaga tgcgcgagcc gcgaaagaac ctgccgcgag
     1201 cgctagtgtt gggagtggtg ggcgtggtgg cgttttatgt gggcgtgacg ttcgcatgtc
     1261 tggcggtgct ggggccggca gggctggctc gaacatctac gccggcttcg gacgtgatgc
     1321 agcgcgtgtt gggcagcaag ggcgcagcat tcatggctgt ggggattgcg atttcgacgc
     1381 tgggattttt gagccagtcg gtgctgactt acccgcgggt attttttgcg atggcggcgg
     1441 atggggtgtt ccccgcggga gtggcgcggg taaatgcgcg aacgcaagtg ccggtggtgg
     1501 cgattctgct ggcgggtctg ctgacagtgg cggtggtgct ggctggaaat tacgatgcga
     1561 ttctggttta tgtggaatcg atggacggcc tgttcttcgg gctgagcgcg ctggcgctgt
     1621 tggtgctgcg gaggcgggca gcggcggcgg ggatgcaggc ggtatttcga gcgccggggc
     1681 atccttggac cacgctgatt ttcgtggctg cgtattgggt ggtggtggcg acttcgtttt
     1741 acaagtaccg ggcaaatgcg gcgctggtaa tggcgatttt gctggcgggg gttccgctat
     1801 atttttattg gagcaggcca aggaaggcgc ccgcctcaga aggcggccgc tacgtaggag
     1861 aggaatgagt taccggagga cgcaagcgcg aacggcgtat atcgagtggg cgaagctgag
     1921 cgcgtcggca aaatttaatt tggcggcgag tggcgtgaaa gggttgccgc tgcgcgagtt
     1981 gccgctgccc tgggatgagc tggagctttc ggcgccggga ggatacggct acccgccatt
     2041 gctggagcgg ctggcggcgc gctatggagt ttcgacgaag aatgtggtga cggcaatcgg
     2101 tacctcgatg gcgaattacc tggccatgtc tgctgtgctg gagccaggcg acgaagtggt
     2161 gatcgaggat cccgcttacg acccgatggt tgaagttggc cattatctgg gcgcggtggt
     2221 gaaacggatt ccgcgccggg cggaaaacaa atttcagatt gatgtggatg agcttaaaga
     2281 gattgtttcc ggcaagacgc ggctaatagt cctgacgaat ctgcacaatc caagcggcgt
     2341 gctgctggag gcggaggcgc tacgcgagat tggcgaagtg gcgcgcagcg tgggcgcgct
     2401 ggtgatggtc gacgaggtat atctggagct gctgtttgac cggccgctgc ggccggcatt
     2461 tcatttgggc tcgcatttta tagtgacgag cagtttgacc aaggcttacg ggctatcggg
     2521 attgcggtgc gggtggattc tggcggagcc ggaactggcg aaacgaatgt ggcggcagaa
     2581 cgacctgttc ggaaatattc cggcgcatat tacagagttg ctgagcgtgg tagcgctgga
     2641 tcatctggaa gccattcgtg cgcgtgcgaa ggacttgttg gcccgaaatt ggaatctatt
     2701 ggacgcgttt ctggcggagc atcccgagct ggacgtggtg cgcccgcaag gcggaacgat
     2761 cgtttttccg cgcgtgccga acggggatgg cgatgcattt tgcaaacatc tgagcgggaa
     2821 gtacgaaacg tcggctgtgc caggcagctt tttttccatg cccgatcgca ttcgcatcgg
     2881 gatctgcaat gacccagtcg aagtagagga gggactaagg cgggtgggcc ggttactcct
     2941 ctaagggcga cagagatttc gagtaagtgg ccatgtcttt atcgccgcgg ccggagaggt
     3001 tgaggatgac gacgtcgcca ggcgtgagga gcgagggaga agacccaggt tcagagaccg
     3061 aacctggggc acccgcagac ctagccgagg ccggagagat gcgttcgatg agg
//
LOCUS       DASTPM010000020         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1182899, whole genome shotgun sequence.
ACCESSION   DASTPM010000020 DASTPM010000000
VERSION     DASTPM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182899"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..434)
                     /locus_tag="VFR42_00300"
     CDS             complement(12..434)
                     /locus_tag="VFR42_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367618.1"
                     /translation="MGISLEILWSDEDLFAVKVVTWNGSFGAVAKLYVEIGGLDKLAA
                     IISGFPKDANDARETVLGAFGREYAGGAASLRLFCIDRAGHAYVECKVESGEGSAGVF
                     QTATLIMPVEASAIDRFVIQLRKLERDKQGAAVLVGQG"
     gene            610..1869
                     /locus_tag="VFR42_00305"
     CDS             610..1869
                     /locus_tag="VFR42_00305"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU0367619.1"
                     /translation="MPSHTKLVLGMMSGTSADGIDAALVEISGAPPNLKVNLLNHIHQ
                     SFPPQVRKEILRVAEQNPLTPREFSQLHARLGHAYAQAALAACKKFRISPKNIDLIGN
                     HGQTIFHQGRPTNFLGKPTASTLQVGDGSIIANLTAITTVSDFRPADMALGGQGAPLV
                     PFADYTLYRHPKLGRISLNIGGIANITVIPANAKPSDVFAFDTGPGNMLIDALVQHFT
                     HARRRYDKNAVLARRGHLNRTLLAQLMRDPYFKLKPPKSTGREYFGVSYVKRLLSHAR
                     RYKLKPHDLVHTATVFTAASIAESIHRFVLPSHKIHQLIVSGGGARNPLLLSQLAALL
                     GPSRFVISSEASNLLSGHVSVASRRSSTIDVLPSTEFHIPINAKEALAFAILAYETFH
                     QRPSNIPSATGARGPAMLGKISYAPSR"
     gene            1853..3544
                     /locus_tag="VFR42_00310"
     CDS             1853..3544
                     /locus_tag="VFR42_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0367620.1"
                     /translation="MHRPANFAFQPKRRLTPSEARNLSYLRVSANSAPLRYLSPAASL
                     AARHSPLATFFLLVTLSTLFSSCSHPAPFTGRDLTFLIEANPVNLDPRFATDGQSQRL
                     DGLIFSGLLARDAQMDLHGDLADSWQTPDPLTYIFNLKPNIKFHDGRPLTSSDVKSTF
                     DFILNPANKSPKRGAFRMVDKIEAPDPQTVIFHLKEPYASFLVNLIPNAAGIVPANAQ
                     PDFARHPIGSGPFQFVSQSQDEDVRLIRNADYFNHAPSLESVHFRIVPDAVVRALELR
                     KGSADLEMSSLSPDLIPVLIKQPTLALTQRPGTNFAYLGINLQDPLLASKEVRQALAF
                     ATDRAALIRYLLHGQAQVASGILPPNHWAYEPNVTQYSLDAARAEKLLDAAGFPRKEN
                     GVRLHLTLKTSTEEQARLIGAALQEQWRSVGVDLELRPLELATLLSDAARGNFQLTYA
                     RWVGANNDPDIFEFVFSSRRFPPDGANRGHYRNPRIDALTDQIHVESNHEKRKQLCSE
                     VQKIVADDLPYIPMWFTDVVSVHRRELGDLPVSPTGDYDFLISLRPQVATTSTVR"
     gene            complement(3632..4246)
                     /locus_tag="VFR42_00315"
     CDS             complement(3632..4246)
                     /locus_tag="VFR42_00315"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU0367621.1"
                     /translation="MSAQSPAAEALRYAAERLQHGEVVAIPTDTVYGLAADPFNLSAV
                     EEIFRVKGRQEDRALPILVNSLDQAMLLAREVPRNFLRLAEEFWPGALTLVVDASHRL
                     PLKVTANTGRIALRWPRSEVVGRLIEECDSPLTGTSANLSGSPACTDADMVMHQLGDR
                     LGLILDAGPTKSASPSTIVELHGDDWKILREGAIAAQEIEAALR"
BASE COUNT          963 a         1708 c          953 g          936 t
ORIGIN      
        1 gccgcacgcc actaaccttg gcccacgagt acagccgcac cctgtttgtc ccgctcgagc
       61 tttctcagct gaatcacgaa acgatcaatt gccgacgcct cgaccggcat gatcaacgtc
      121 gcagtctgaa agactccagc cgacccctcg ccggattcga ctttgcattc aacgtaagca
      181 tgccccgctc ggtcgataca gaacagcctg agtgatgcgg caccacccgc atactcccgg
      241 ccaaacgcgc cgagaacggt ttctctagcg tcatttgcgt ccttggggaa tccagaaata
      301 atcgcggcga gcttgtcgag tccgccgatc tcaacgtaca gcttcgcaac tgccccgaac
      361 gacccattcc atgtcaccac cttcacagca aagagatctt cgtccgacca gaggatctcc
      421 aggctgatac ccaccgaatt ttcgtattgt ctcaattcca caaagccacc tcgaacgcgg
      481 ccggtagagc tggtctatca gccgtgctgg ctggcctttt tttgctcatt cactcttagc
      541 actccgtccc tatcttcctc tctgtgtcct cagcgcgctc tgtgttaaaa aattctttca
      601 accattctca tgccctcaca cacaaaactt gtcctcggca tgatgtccgg cacgtccgcc
      661 gacggcatcg acgccgccct cgtcgaaatc tccggcgcac ctcccaacct gaaagttaat
      721 ctcctcaacc acatacacca atcgttcccg ccgcaagtcc gcaaagaaat cctccgcgta
      781 gccgaacaaa atcccctcac gcccagagaa ttcagccagc tccacgcccg cctaggccac
      841 gcctacgcgc aagccgccct cgccgcctgc aaaaagttcc gcatctcccc caaaaacatc
      901 gacctaatcg gcaaccacgg ccaaaccatc ttccaccagg gcaggccaac aaattttctc
      961 ggcaagccaa ccgcctccac cctgcaagtc ggcgacggct cgatcatcgc caatctcacc
     1021 gccatcacca ccgtctccga cttccgcccc gccgacatgg cgctcggcgg ccaaggcgca
     1081 ccgctggtcc ctttcgccga ctacactctt taccgccatc ccaaactcgg ccgcatcagc
     1141 ctcaacatcg gcggtatcgc caacatcacc gtaatccccg ccaatgccaa accttccgac
     1201 gtcttcgctt tcgacactgg ccccggcaac atgctcatcg acgccctggt ccaacacttc
     1261 acccacgccc ggcgccgcta cgacaaaaat gccgtgctcg cccgtcgcgg ccatctgaat
     1321 cgcaccctcc tagcgcagct catgcgtgat ccctatttca agctcaaacc gcccaaatcc
     1381 accggccgcg aatatttcgg cgtctcctac gtgaaaagac ttctatccca tgcccgccgc
     1441 tacaaactaa aaccgcacga cctcgttcac acggcaaccg tctttaccgc cgcatccatc
     1501 gccgagtcca tccaccgctt cgtccttccc agtcacaaaa ttcaccagct catcgtctcc
     1561 ggcgggggcg cccgaaaccc cctcctgctc tcgcaactcg ccgccttgct cggcccgtcg
     1621 cgcttcgtca tttcgagcga agcaagcaat ctcttgtctg gtcacgtgtc agtcgccagt
     1681 cggcgctctt ccaccatcga cgtgctgcct tctaccgaat tccacatccc catcaacgcc
     1741 aaagaagccc tggccttcgc catcctcgcc tacgagacct tccaccagcg cccctccaac
     1801 attcccagtg ctaccggcgc acgcggcccc gccatgctcg gtaaaatctc ctatgcaccg
     1861 tcccgctaat tttgcatttc agccaaaacg ccgactcact ccgagcgaag cgaggaatct
     1921 ctcttatctg cgcgtctccg cgaactctgc gcctctgcgt tatctttctc ctgccgcttc
     1981 actcgccgct cgccattcgc cactcgccac tttttttctc ctggttacac tttccacgct
     2041 attcagcagt tgctcccatc cagcgccctt caccggccgc gacctcacct tcctcatcga
     2101 agccaatccc gtcaacctcg atccccgctt cgccaccgac ggccaatctc agcgcctcga
     2161 tggcctcatc ttctccggcc tgctcgctcg cgacgcacaa atggatctcc acggcgacct
     2221 agccgactcc tggcaaactc ccgacccgct cacttacatc tttaatctca agcccaacat
     2281 aaaattccac gacggccgcc cgctcacctc tagcgacgtt aaatccacct tcgatttcat
     2341 cctcaacccc gctaacaaat ctcccaagcg cggcgccttc cgcatggtcg acaaaatcga
     2401 agctcccgat ccgcaaaccg tcatctttca cctcaaggag ccttacgctt ctttcctcgt
     2461 caatttgatt cccaacgcag ccggcatcgt ccccgctaac gcgcagcccg atttcgcgcg
     2521 ccatcccatt ggctccggcc ctttccaatt cgtcagccaa tctcaggacg aagatgtccg
     2581 gctgatccgc aatgcagatt atttcaacca tgcgccatct ctcgaatctg ttcacttccg
     2641 catcgtcccc gacgccgtgg tccgcgctct cgaactccgc aaaggttccg ccgacctcga
     2701 aatgagttca ctatctcccg acctcattcc ggtcctgatt aagcaaccaa cgctcgcgct
     2761 cacccagcgc cccggtacga acttcgccta cctcggcatc aatctccaag acccgctcct
     2821 cgctagcaaa gaagttcgac aagccctggc cttcgctacg gatcgagctg cattgattcg
     2881 atatttgctg cacggccagg cgcaagttgc cagcggcatc cttccgccca atcactgggc
     2941 ctacgaaccc aacgtcacgc aatactcgct cgacgccgct cgcgccgaaa aacttctcga
     3001 cgccgctggc ttcccacgca aagaaaacgg agtgcgcttg cacctcactc taaaaacttc
     3061 gaccgaggaa caagcacgcc tgattggcgc agcattgcag gaacagtggc gcagcgtcgg
     3121 tgtcgatcta gaacttcgcc cgctcgaatt ggccactttg ctttccgatg cggcacgcgg
     3181 aaattttcag ctcacttacg cccgctgggt cggcgccaac aacgatcccg acattttcga
     3241 atttgttttt tcgtctagac gtttcccgcc cgacggcgcc aaccgcggac actaccgcaa
     3301 tccgcgcatc gacgcgttaa ccgatcaaat ccatgtggag tccaatcatg aaaaacgaaa
     3361 acaactttgc agcgaagtac aaaaaatcgt cgccgacgat ctcccctaca tccccatgtg
     3421 gttcacggat gtagtctccg tccaccgccg cgaactaggc gacctgccgg tctctccaac
     3481 cggcgactac gattttctca tttcactaag gccgcaggtc gcgacgacct cgacagtccg
     3541 ctagctgcaa agcaaacgtc ccgtgaacag ttcaaccaaa gagaaacggc ggcccgcaac
     3601 gagggaggcc gattttcaac tttcgatttc ctcagcgaag cgcggcttcg atttcctgcg
     3661 cagcaatcgc accctcgcgc aaaatcttcc agtcatcgcc atgcaattcc acaatcgtgg
     3721 aagggctggc cgatttggtt gggccagcat ccaggatcaa tccgagccgg tcgcccagtt
     3781 ggtgcatcac catatccgcg tccgtacagg ccggcgaacc cgacaaattc gcactggtcc
     3841 ccgtcagcgg actgtcgcat tcttcgatca gtcgcccgac cacttcgctg cgtggccaac
     3901 gcagcgcaat tcttcccgtg ttcgcggtaa ccttcagcgg cagccggtgc gaagcatcca
     3961 ccactagcgt cagcgcgccc ggccagaatt cttccgccag ccgcaaaaaa ttccgcggta
     4021 cttcccgcgc cagcagcata gcctggtcca gcgaattcac taaaatcggc agcgcgcgat
     4081 cctcttgccg gcccttcacc cgaaaaatct cctcgactgc cgacaaatta aacgggtccg
     4141 cagcaagtcc atacacggtg tcggttggaa tggcaaccac ttctccgtgt tgcagacgtt
     4201 cggcggcgta ccgcaacgct tccgccgctg gcgactgcgc gctcacgcgc aaaatctccg
     4261 ccgacaatgc cgtgcctctc gcgaagtagt tcggcccgct gcagcgctcc gcgcccgctg
     4321 ccacgctgcg cgcgacgcta ccacacacct actaacgacg caataactct ctcatttccg
     4381 tcatttccgc tccacgtcgc agcgccttta cgtcgctaaa accgtcacaa caaaaaacgc
     4441 gccaacccga tatgcaggtc cgcgcgcttt tgtaatcttc ttcttttctt acttcgttgc
     4501 ttccgtatct aatccttctt cgccgacccc tgggcctgtt gcggcacata caccgacccc
//
LOCUS       DASTPM010000021         4064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_317987, whole genome shotgun sequence.
ACCESSION   DASTPM010000021 DASTPM010000000
VERSION     DASTPM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317987"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="VFR42_00320"
     CDS             complement(<1..1693)
                     /locus_tag="VFR42_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEU0367622.1"
                     /translation="MATQIDRSNVDKLKIAWTYRTGALEQSKRLIHKAAFEATPILVE
                     GKLFLSTPYNHVIALDPGTGEKLWEYDPAVNLERNYSEVSSRGVSAWHDTRAKAGEPC
                     RLRIYAGTLDARLIALDGKTGRLCGSFGTSGTVDLNQDAATQTEWTGGYQVTSPPAIF
                     RDLVIVGSSIADNWKVDTGRGIVRAFDARSGKLRWTWDPIPWGQKTQPRTGAGNAWAV
                     LSVDVEHDLVFVPTGAAAPDYFGGIRKGDNKWANSVVALRASTGEFVWGFQVVHHDLW
                     DYDVASQPTLFSWKDGTPAVVINTKMGHVFVLNRLTGAPLLPVEERKVPQSDIAGEES
                     SPTQPFSTISLVPEKFEAADVWGPKPEDVKWCQDKIAASRNEGIFTPPSLKGTVQAPG
                     NVGGVNWGSAAYDSQRHLMIANTNRLVAWMKLIPRAGYDSEVKRDQDNRIYGEFGDQD
                     PSAYGMYRTFLFSPSGTPCNAPPWGTAEAVDLFTGKIVWNVPLGTMVAGKATGSINLG
                     GPLMTAGGLVFTSATIDPHLHAFDSETGKELWNHELPAAAQATPMTYMWKGKQYLV"
     gene            1782..2090
                     /locus_tag="VFR42_00325"
     CDS             1782..2090
                     /locus_tag="VFR42_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367623.1"
                     /translation="MQTIPAIAALQTLGNRCADTRFTLFIACPHPAITIRHQPGHYRY
                     YPSLIYTTSIEELLQKFFAPPSNHFFLQGILIVHVLPSRGLEFAAKTLSRAAAWDFHF
                     "
     gene            2220..>4064
                     /locus_tag="VFR42_00330"
     CDS             2220..>4064
                     /locus_tag="VFR42_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367624.1"
                     /translation="MASLLVVAPPCLAQESSRPSGPGWVVIGLDEYKTLRAKAYPPER
                     EPEPPPVEATLSRVDYDLHINGEFASGRANLTIDVLKDGWVRVPIPAGLLVREAKLDG
                     KSVSLVSGPAGKSGGQLSAVLSHSGRSTLILDVALPISANAGEESISLPPSASGVTRA
                     TLELPRQGVDIRLNGGLLSDRSETQNTNRWIVYATGNAPLIFSWKRKTEDHRGELTLR
                     LRGNLTQILGLGEDSASLTAEVTLEIAQGLAREVKIQVPQQITINQVLGASVADWESH
                     PSEKSGQESGELTVTFLEPVDQSARFVITGETRLPRDGQIAIPLLKLLNTERISGGAA
                     VEVLGAGEIKDVKTQGFENADASDLGELVANRQSPSLAAFRLRAGDANQPRALTINVA
                     RYDQQAVLLANIEEARYRVLLSKEGKTLVQARYAVRNNQRNFVKIALPAGATLWSAAL
                     SGQPVRPGQAPDGSLLLPLEKSKAGGSTADDPPEFAVEIVYLTPGASWTDKGKARIAL
                     PALDLPVSRTGLQVYYPPLFRVTSEPGIFHTETYAAPTSPALSATLDGESRSVLLKSA
                     PGAGAAGGMARGIAGGSFQTGIGTDAGVAPPTNLPIEGRNMAAAQVVSP"
BASE COUNT          793 a         1421 c          995 g          855 t
ORIGIN      
        1 tcaccaggta ctgcttgcct ttccacatgt atgtcatcgg cgtggcttgc gccgccgccg
       61 gcaattcgtg attccataat tccttgccgg tctcggaatc aaacgcgtgc aggtgcgggt
      121 cgatcgttgc agaggtaaaa accagtccac ccgccgtcat cagcggtccg ccaaggttaa
      181 tcgagccggt cgcctttccc gccaccatgg tacccaacgg aacgttccac acaatcttgc
      241 ccgtaaataa atctaccgcc tccgcggtgc cccacggtgg agcgttgcag ggcgtgccgc
      301 tcggggaaaa caaaaatgtc cggtacattc catacgccga cggatcctga tcgccgaact
      361 cgccgtaaat ccggttgtcc tggtcgcgtt tcacctcgga gtcatagcca gcgcgcggga
      421 tcaatttcat ccaagcaacc agccggttgg tattcgcgat catcagatgg cgttgcgaat
      481 cgtacgccgc gctgccccaa ttcactccac ccacatttcc cggcgcttgc accgtccctt
      541 ttaggctcgg cggcgtaaaa attccttcgt tacgggacgc cgcaattttg tcctggcacc
      601 atttcacatc ctcaggcttt ggcccccaga catccgccgc ttcaaatttt tccggcacca
      661 gcgaaatggt cgagaacggt tgcgtgggcg aactctcctc gcccgcgata tccgattgcg
      721 gaacttttcg ttcctcgacc ggcagcagcg gcgctcccgt cagtcggttc aacacaaaca
      781 cgtgccccat cttggtgttg atgaccaccg ccggcgttcc atccttccag gaaaacaacg
      841 tcggctgcga cgccacgtcg tagtcccaca agtcgtggtg cacgacttgg aatccccaaa
      901 cgaattcgcc tgtcgaagcc cgcaacgcca ctacagaatt cgcccacttg ttgtcgcctt
      961 tccggatgcc gccaaaataa tccggcgccg ccgctcccgt cggcacaaac accagatcgt
     1021 gctccacgtc gaccgaaagg actgcccacg cattcccggc gcctgtacga ggctgcgtct
     1081 tttgccccca cggaattgga tcccacgtcc aacgcagctt tccgctgcgg gcatcgaacg
     1141 cacgaacaat tccccgcccc gtatccacct tccagttatc cgcaatcgaa gatcctacaa
     1201 tcaccagatc acggaagatc gccggcggcg acgtcacttg atacccgccc gtccactccg
     1261 tctgcgtcgc agcatcttga tttaggtcaa ccgtgccgct tgttccgaaa cttccgcaaa
     1321 gtcttcccgt tttcccgtcc aacgcaatca gtcgcgcgtc cagcgttcct gcatagatac
     1381 gcagccggca cggttcgcca gctttggccc gggtgtcgtg ccacgccgaa actccgcgtg
     1441 aagaaacctc cgaatagttc cgctccagat ttaccgcagg atcgtattcc cacaatttct
     1501 cgcctgttcc agggtcgagc gcaatcacgt ggttatacgg cgtgctcaaa aacagtttgc
     1561 cctccaccag aatcggcgtc gcttcgaacg cggccttgtg aatcaggcgc tttgactgtt
     1621 ccaacgcgcc cgtgcggtag gtccacgcga ttttgagctt atcgacgttg cttcggtcga
     1681 tctgggttgc cacggaatac cgcgttccac ccgcgtcgtt tccatacgtc ggccagctgg
     1741 catctgcctt cccctgccct aagacacttc ccgcacagct gatgcaaacg attccagcca
     1801 tcgctgcatt gcaaacgctt ggaaatagat gcgccgatac ccgcttcact cttttcatcg
     1861 cttgtcctca tcccgcaata acgattcgcc accagcccgg ccattaccgc tattaccctt
     1921 ccttaatcta cacgacctct atagaggagt tgctgcagaa attttttgcg ccgccaagca
     1981 accatttttt cctgcaagga attctcatcg ttcacgttct accttcgcgg ggcttggagt
     2041 ttgcagcaaa aacgttaagc cgagccgccg cctgggattt tcacttctga tccccggtta
     2101 atcccggatc gtaggtttgt gcctagaaac tctggccgaa caaggacacg cacatgacca
     2161 actctctggt tcttcttgtt tcaggaagat cgctctcgaa ctgtctcacg atccccatca
     2221 tggcttcact tctcgtcgtc gcgccccctt gtctcgcgca ggagtcctcg cgcccgtccg
     2281 gtcccggctg ggtggtcatc ggcctcgacg aatacaaaac tctgcgggcc aaagcctatc
     2341 ctcccgaacg cgaacccgag ccgcctcccg tcgaagccac tctatctcgt gtcgattacg
     2401 atcttcacat taacggcgaa tttgcctccg gccgcgcgaa tctcacgatt gacgttctca
     2461 aggacggctg ggttcgtgtt cccatccccg ccggacttct tgtccgcgaa gccaagcttg
     2521 atggcaaatc cgtatcgctc gtttccggtc ctgccggaaa atccggcgga cagctctccg
     2581 cggtactctc tcattccggc cgctctactc tcatcctcga cgtcgctctt cccatttccg
     2641 ccaacgccgg cgaagagagc atttccctgc caccttccgc ttccggcgtc actcgcgcga
     2701 cactcgaact tccgcgccag ggcgtggata ttcgcctcaa cggcgggttg ctctccgatc
     2761 gctccgaaac ccagaacacc aatcgctgga tcgtctacgc caccggcaac gcgcctctaa
     2821 tattttcgtg gaaacgcaag accgaagacc accgcggcga acttacgctc cggctgcgcg
     2881 gcaacctgac ccagatcctc ggcctcggcg aagactccgc ctcgctcacc gccgaagtga
     2941 ctctcgaaat tgcccagggc ctagcccgcg aagtgaaaat tcaggtgccg cagcagatca
     3001 ccatcaatca agtcctcggc gccagcgtcg ccgactggga atcccatccc agcgaaaagt
     3061 ccggtcaaga gtccggcgaa ctcaccgtca ccttccttga acctgtcgac caatccgccc
     3121 gtttcgtcat cactggtgaa acgcgcctcc cgcgcgacgg gcaaatcgcg attcctcttc
     3181 tcaagctgct caataccgag cgcatttcgg gtggtgctgc cgttgaagtc ctcggtgccg
     3241 gcgaaatcaa ggatgtcaaa acgcaaggct ttgaaaacgc cgacgccagc gatcttggcg
     3301 aactcgtcgc caaccgccag tcgccgtcgc tcgccgcctt tcggcttcgt gccggtgacg
     3361 ccaaccagcc ccgcgccctc accatcaacg ttgcccgtta cgaccagcag gcggtcctgc
     3421 tcgctaacat cgaagaagcc cgctatcgcg tcttgctcag taaggaagga aaaacgcttg
     3481 tccaggcgcg ctacgcggtc cgcaacaatc agcgcaactt cgtaaaaatc gcgctacccg
     3541 ccggtgccac cctgtggagc gccgcgcttt ccggtcagcc ggtccgcccc ggccaggcac
     3601 ccgacggcag tctcttgctt cccctcgaaa aatctaaagc tggcggcagc acagccgacg
     3661 atccgcccga attcgccgtc gaaatcgtct atctcactcc cggcgccagc tggaccgaca
     3721 aaggcaaagc acgcatcgcg cttcccgctc tcgacttgcc cgtctcccgc accggcctgc
     3781 aggtttacta cccgccactt ttccgagtca cttccgagcc aggtatcttc cacacagaaa
     3841 cctacgccgc cccgacctct ccagcactaa gcgcgacgtt ggacggcgag tctcgctcag
     3901 tcttgctcaa gtcagctcca ggggccggcg cagccggcgg catggcccgt ggaattgctg
     3961 gaggctcgtt ccagacgggt atcggcaccg acgcgggcgt ggctccgccg acgaatcttc
     4021 ccatcgaggg ccgcaacatg gctgcggcgc aagtcgtgtc tcct
//
LOCUS       DASTPM010000022         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_144983, whole genome shotgun sequence.
ACCESSION   DASTPM010000022 DASTPM010000000
VERSION     DASTPM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144983"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2428
                     /locus_tag="VFR42_00335"
     CDS             <1..2428
                     /locus_tag="VFR42_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367625.1"
                     /translation="SGTGLVDQYGRAISSGIEEPLAAADPVVRKVSGSIREVGVAAEG
                     TVPKMAAASAAIRTGFGEQSIRAVERFVSMIPGVGVALQAIFPVVGALALVEMLGKVF
                     EKSEKVTEAEKAMADATKQAHDAFTTMAQTIDRLAAKDIGRAFGPAAGAAAEIRAMEL
                     QKDDLYKDLQKAKDQLGALATEAQGKILKRPTGGSLIEEEYKPKFEEAGKAIEAAQSK
                     VTEFEARIKDARAQAAELAKTESGSLTAIEVSNAEKSAERRAEAVKSAALARIRSEHD
                     VRQAVIDSEQDAGTREIATAEERVRLAQNTATAEITYEQSVTKAKVDGINARAAAESR
                     GKPRSEVDKINATAGGEVAAAKDEGLQKGLDAQRAVDAEESKIGIAHAAAERGWAAET
                     TRAWEEAGRKREEAAKKAAEEDNREAELAAKRASAVSRVSEFQAKSKGDLAALATKQK
                     EIALEGEYDAKIAHSLSDEISYMRQIAELQHEQRAAEIAGVQAALDEAEAVSGELRDL
                     RRIAELKAQIAKLTQEDKNASEEAANKAAKMAKDKSLAGQVASKLPQGSLEDKTTAAA
                     ADSIAQAVDGISSALGRSVVQGKNLGKALADAGKQLAMTTATQVLKAGLSSAVNALMQ
                     MIPAFHATAAAATASEAAQKAAAAAGVAAQKTLAEASVMTAAGEAAAWGFESVMAALP
                     FPVNIAVAPAIAASALAQTEAYGQFAAGTDSAPGGMALVGEKGPELVNLPRGSQVIPN
                     HKIMQYANGTPGYSSTTHYQSTSFQTGSTELHFHAHGMSNPDRFIDHVMRKIPEALKR
                     RSSIFAPASR"
     gene            2430..>3233
                     /locus_tag="VFR42_00340"
     CDS             2430..>3233
                     /locus_tag="VFR42_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367626.1"
                     /translation="MSWTERARADIGSSGVNGATIYVADAPAAVAATYSASSEVSCVV
                     EDMNGILAHKGITGAMLQQTAGASLGTNLTTFTQTFPGSVTLGDAIIVALTCAPNAFG
                     STPITITDSLGNTYTEIYNHILTGGSIQLGIWIAISAASGANTVTATSPGGGGPFSSE
                     FAAPAMAILEYPGHATVDTLVVATSATGGTLPVSITTGGNGETLLNIVGLGPTCAPPS
                     PVLTGPSGGINIPEAWLIVYEYGGSPATGSYVDRSKFLFLGQGGQHTFNL"
BASE COUNT          736 a          912 c         1043 g          542 t
ORIGIN      
        1 ttccggcacc ggcctggtgg accagtacgg gcgcgcgatc tcatcgggca ttgaagagcc
       61 tctcgccgcg gctgatcccg tggtccgcaa agtttcgggc agcatccgcg aggtgggggt
      121 cgccgctgaa ggaacggtcc cgaagatggc ggcggcctcg gcggctatcc gaaccggatt
      181 cggggagcag tccatccgtg cggtcgagcg tttcgtttct atgattcctg gcgttggagt
      241 agcgctgcag gctatctttc cggtcgtcgg cgcgctcgct cttgtagaga tgctgggcaa
      301 ggtcttcgag aaatccgaga aggtgacgga agccgagaaa gcgatggccg acgccaccaa
      361 gcaggcccat gacgctttta ccacgatggc gcagaccatc gacaggctcg cggcaaagga
      421 cattggccgc gccttcggtc cggcggccgg cgcggcggcg gaaatccgcg cgatggagct
      481 gcagaaagac gatctctaca aggacctgca gaaggcgaaa gaccagctcg gcgcgctcgc
      541 cacggaggct cagggaaaga tactgaagcg cccgaccggc ggctcgttga tcgaagaaga
      601 gtacaagccg aagtttgagg aagcgggaaa agcgatcgag gccgcgcagt ccaaggtgac
      661 ggagtttgaa gcgcggatta aagacgcacg cgcgcaagct gcggaactgg caaaaacgga
      721 atccggctca ctgaccgcta tcgaagtgtc gaatgctgag aagtctgcag aacgtcgggc
      781 agaggcagtg aagagcgcag cgctggcccg cattaggtcc gaacacgatg tccgacaggc
      841 ggtcatcgat tcggaacagg atgctggtac gcgcgagatc gcgactgcag aggagcgtgt
      901 tcgtcttgca cagaacacgg ccactgcaga gatcacatac gaacagtctg tcaccaaagc
      961 caaggttgac gggataaacg cgcgcgcggc ggctgagtct cgcggtaaac cgcgttcgga
     1021 agtggacaag atcaatgcga ctgccggtgg cgaggtcgcc gcggcgaaag acgaaggtct
     1081 gcaaaaaggc ctcgacgcac agcgggcggt agatgcggaa gaaagcaaga tcggaattgc
     1141 tcatgctgct gcggaacgag gatgggcggc ggagacgacg cgcgcatggg aagaagctgg
     1201 aagaaagcgc gaagaagccg cgaaaaaagc tgccgaggaa gacaatcgtg aggcggaact
     1261 tgccgcgaaa cgcgcgtctg cggtttcgcg cgtttctgag tttcaggcca aatccaaggg
     1321 agacttagcg gcgcttgcga caaaacaaaa ggaaatcgct ctggagggcg aatacgatgc
     1381 caaaatagcg cattctctgt cggatgagat ttcgtatatg cgccagatcg cggagctgca
     1441 gcacgagcag agagcagcgg agatagcggg cgtgcaggcg gcgctggatg aagcggaagc
     1501 tgtgtccggc gaactacgcg acttgcgtcg catcgcagaa ctgaaagcgc aaatcgccaa
     1561 actcacgcag gaagacaaaa acgcatccga agaggcagcg aataaagcgg caaagatggc
     1621 aaaggataag tcgctggcgg ggcaggtggc ctccaagcta ccgcagggat cactcgaaga
     1681 taagaccacg gcggcggcag cagattccat cgcacaagca gtagacggga tttcttccgc
     1741 tctgggacga tctgtagtgc aaggaaaaaa tctgggcaaa gccctggccg acgcgggaaa
     1801 acagttggcc atgacgactg ctacccaagt gctgaaagcc ggcttatctt cggcggtcaa
     1861 tgcgctgatg cagatgattc cggcatttca tgcgacggct gcggcggcta ccgcgtctga
     1921 ggcggcccaa aaggccgctg cggcagcggg cgtcgcagct cagaaaacgc tggcggaagc
     1981 atccgtaatg acggcagcag gagaagctgc tgcatggggt ttcgagtcgg tgatggctgc
     2041 gctacctttt ccggttaaca ttgctgtcgc tccagcgata gctgcaagcg cattggcaca
     2101 gacggaggca tacggtcaat tcgccgctgg aactgattcg gcacccggcg gaatggctct
     2161 cgtaggcgaa aaaggtccgg agcttgtcaa cctgccgcgc ggctcgcagg tgatcccgaa
     2221 tcacaaaatc atgcaatacg ccaacggcac gcctggttac tcttcgacca cgcactacca
     2281 gtccacatcc tttcagaccg gctcgacgga actgcacttc cacgcgcacg gcatgagcaa
     2341 cccggacagg tttatcgatc acgtcatgcg caaaataccg gaggcactga agcggcgaag
     2401 ctcgatattc gctcccgctt cccgatgaca tgagctggac cgagcgagcg cgggcagata
     2461 ttggcagcag cggcgtaaac ggcgcgacga tctacgttgc tgacgcgccg gcggccgtcg
     2521 ctgcaacgta ttcggccagt tcggaggttt cctgtgttgt cgaggacatg aacggcatcc
     2581 tcgcgcacaa agggatcacg ggcgcgatgt tgcagcagac tgcgggcgct tcgctcggca
     2641 caaacctcac cacgttcacc cagacgtttc ctggcagcgt cacgttgggg gacgccatca
     2701 tcgtcgctct gacgtgcgcg cctaatgcct tcggctcgac tccgatcacg attaccgaca
     2761 gcctgggaaa cacgtacacg gaaatctaca atcacatcct cacgggcggg tcgatacagc
     2821 tcggcatctg gattgcgatc tcggcggcta gtggcgcgaa caccgttacc gcaacctcgc
     2881 ccggcggcgg cggtccgttc tcgagcgagt tcgcggcgcc cgcgatggct atcctcgaat
     2941 atcccggtca cgcgaccgtg gacactctgg tggttgccac ttctgcgaca ggcggaacgc
     3001 ttccggtttc gatcactacg ggcgggaacg gcgaaacgct gctcaacatc gttggcttgg
     3061 gaccgacctg tgcgccgccc tctccggtgc tgacgggtcc cagcggcggt atcaacatcc
     3121 cggaagcgtg gctgatcgtt tacgaatacg gcggatcgcc cgcaaccgga tcatatgttg
     3181 accgcagcaa gttcctgttt cttggccagg gcgggcagca cactttcaat ctg
//
LOCUS       DASTPM010000023         3785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_173833, whole genome shotgun sequence.
ACCESSION   DASTPM010000023 DASTPM010000000
VERSION     DASTPM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173833"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFR42_00345"
     CDS             complement(<1..502)
                     /locus_tag="VFR42_00345"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0367627.1"
                     /translation="MPRAALKLHPNDNVLIALRDLHKGDPISVAQHSFSLTSDVPAKH
                     KFAATDLAPGAAVLMYGVLVGKATQSVPQGSALNVLNIRHDALPFHENVSDYRWDAPD
                     VSRWRDRTFLGYRRADGQVGTRNYWLVVPLVFCENRNIETLKQAFEEELGFAAPRVYR
                     RQVADLV"
     gene            669..1763
                     /locus_tag="VFR42_00350"
     CDS             669..1763
                     /locus_tag="VFR42_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU0367628.1"
                     /translation="MALKIKEKKDCRWDLVSLGEVMLRFDPGDRRIATTRSFDVCEGG
                     GEYNVARGLKRCFGLETAVITAFADDPVGRLLQDFIYQGGVDQSLVRWVAHDGVGRSA
                     RNGLNFTERGFGVRAALGSYDRGHTAVSQLKPGDIDWDGIFGKHGARWLHTGGIFSAL
                     SSTTPDVALEAMQSAKRFGTIISYDLNYRPSLWKSIGGKKQAQEVNRRIAPLVDVMLG
                     NEEDFSAALGYEVAGVDASLSDFEVENFRHMIAKVVKDYPFKVVATTLRKAKTATRND
                     WGAICYSDGDFYQAKERKDLEIYDRVGGGDSFASGLIYGFLADKGPQWAVECGAAHGA
                     LAMTTPGDTSMVTLAEVLRVMEDKGARIAR"
     gene            1783..2469
                     /gene="eda"
                     /locus_tag="VFR42_00355"
     CDS             1783..2469
                     /gene="eda"
                     /locus_tag="VFR42_00355"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0367629.1"
                     /translation="MNKQQVRDRILEVGVIPVVRAKSPTEARLAAQAVCDGGIPIVEI
                     TMTVPGAVEVIRELRKSAPSSVLIGAGTVLDADAARRCIDAGAQFLVSPGLNIPTVEF
                     ASRQEILMLAGALTPTEVITAWSAGSDFVKIFPCGQVGGAKYIRALKGPLPQVPLVPT
                     GGVNLNTAAEFIEAGAAALGVGGELVLADALKSGKPDAIVENARKFAAIVKQARQAAV
                     KPTMPVASAS"
     gene            complement(2480..3256)
                     /locus_tag="VFR42_00360"
     CDS             complement(2480..3256)
                     /locus_tag="VFR42_00360"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0367630.1"
                     /translation="MGYPLLDLKNKTAVVIGGTSGIGLALAKGLAQAGASVVPTGRRE
                     AEAQSAAAAVEALGQKSLAVTCDVTDNGTLQRLLQGVLTKFGSVEILVNCAGRTKRQP
                     TLDVSEADWNVIMETNLNGTMRACRVFGRHMLERRHGRIINIGSLASFVGLYEVAAYC
                     ASKGAVASLTKSLAVEWAKFGVCVNAIVPGVLRPDLNAALLDGTERGREFLLRTPMGR
                     FGKPEELIGAAVFLASDAAGFVTGHLLAVDGGILASGVNQ"
     gene            complement(3348..>3785)
                     /locus_tag="VFR42_00365"
     CDS             complement(3348..>3785)
                     /locus_tag="VFR42_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367631.1"
                     /translation="LYYAVGVFSEKLPDGTVNGIPVFDQSWKNVVATFYHELNEARTD
                     PDVEDAIRAGDDPAAVNFLGWTSKQGEECGDFPVFEANPLTQVFQEVRLTGGSGTVPV
                     QFQYSDHVHGPEGPIKTRPPYAGELQSKSAAQPDSSGRLNKNR"
BASE COUNT          799 a         1118 c         1095 g          773 t
ORIGIN      
        1 tgaccagatc cgcgacttgc cgccggtaca ctctgggtgc cgcgaatcct aattcttctt
       61 cgaatgcctg cttcagagtc tcgatgttgc ggttctcgca aaagaccaac ggaacaacca
      121 gccagtagtt tcgggtgccc acctggccat cggcccggcg gtacccgaga aacgtacggt
      181 cgcgccagcg agatacgtcc ggagcgtccc aacgataatc gctaacgttt tcgtggaacg
      241 gcaaggcatc gtgacggatg ttcagtacgt tcagcgcaga tccttgcgga accgattgcg
      301 tcgccttccc caccaggact ccatacatga gtaccgcggc ccctggcgcc aggtccgtgg
      361 ccgcaaactt gtgctttgct ggcacatccg aagtcaacga gaacgaatgt tgtgcgaccg
      421 aaattggatc acccttgtgg agatctcgca atgcgattag cacgttgtca ttcgggtgca
      481 gtttgagagc cgcgcgcggc atgaacaatc ctcacagagt gttatacccg atcccctccg
      541 aatgcgcccc ttgggcgcga cttgcagacg agcggattca acttgtgtat aacaatacag
      601 aatctcagga acaagttcgc cgcaccgtat ccgcaactcg gcgctggcca gaaaaattcg
      661 aatcgacgat ggcacttaag atcaaagaga agaaagactg ccgttgggat ctcgtaagcc
      721 tcggcgaagt catgctgcgc ttcgatcccg gcgaccgccg aatcgccacc acgcgcagct
      781 ttgacgtatg cgaaggcgga ggagagtaca acgtggctcg cggcctgaag cgttgctttg
      841 ggcttgagac agccgtgatc accgctttcg cggacgaccc ggtcgggcgg cttctgcagg
      901 attttatcta tcaaggcggt gtggaccaat ctctggttcg ctgggtcgcc cacgacggcg
      961 tgggccgctc cgccagaaac ggcctcaatt tcacggagcg cggcttcggc gttcgggcgg
     1021 cccttggctc ttacgaccgc ggacatacag cggtctcgca attgaagccc ggggacatcg
     1081 attgggatgg gatttttggc aagcatggtg ctcgctggct gcacacaggt ggaatcttca
     1141 gcgcactctc ttcgactacg ccggatgtcg cgctcgaggc gatgcagtcc gccaagcggt
     1201 tcggtaccat catttcgtat gacctgaatt accggccttc gctgtggaag tcaatcgggg
     1261 ggaagaagca agcccaagag gtcaatcgtc gtatcgctcc cctggttgac gtcatgctgg
     1321 gcaacgaaga agatttttcc gctgcactcg gctacgaagt agccggggtg gatgcaagcc
     1381 tctccgattt cgaagtcgag aatttcaggc acatgattgc caaggtcgtg aaggactatc
     1441 cgtttaaggt ggtggcgacc accttgcgca aggctaagac cgcaacccgc aatgattggg
     1501 gtgcaatctg ctatagcgac ggcgatttct atcaggcgaa agagcgcaag gacctggaaa
     1561 tctacgatcg cgtcggcgga ggagattcgt tcgcatccgg cctgatctac ggttttctgg
     1621 ccgacaaggg accgcagtgg gcagtggaat gcggcgcagc ccatggcgct ctagcgatga
     1681 cgacaccggg cgacaccagc atggtcacgc tcgcggaggt gcttcgcgtg atggaagaca
     1741 aaggtgcgcg catcgcgcgc tgagcctggc ttggaggatt cgatgaataa acagcaggtg
     1801 cgtgaccgga tcttggaagt tggcgtgatt ccggtggtgc gagccaagtc acccaccgag
     1861 gctcggcttg cggcacaggc ggtgtgcgac ggaggtattc caatcgtcga aatcaccatg
     1921 acggtgcccg gagcggtcga agtgatccgc gaactacgca agagcgcacc ttcctccgtg
     1981 ctgatcggag ctggcacggt gctggatgca gacgcagcgc gccggtgtat agatgctggc
     2041 gcacagttcc tggtcagccc ggggctgaat attccgaccg tagaattcgc gagccgacag
     2101 gaaatcctga tgttggcggg cgcgctcact ccgaccgaag tcatcaccgc atggagtgca
     2161 ggatcggatt ttgtgaagat ttttccgtgt ggccaagtcg gcggcgccaa gtacatcaga
     2221 gcgttgaagg ggcccttgcc gcaggtccct cttgtgccga caggcggcgt caatctcaac
     2281 accgccgctg aattcattga agccggagca gcggctttgg gcgttggggg agaacttgtg
     2341 ctggccgacg ctttgaaatc agggaagccc gacgcgattg tagagaacgc acggaagttt
     2401 gcagcaatcg taaagcaggc tcgccaggcc gcagtgaaac cgacgatgcc ggtggctagc
     2461 gcaagttaga acgtcgaggc tactgattta ccccactcgc gagaatccct ccgtcaaccg
     2521 cgagcaaatg tccggtaaca aagccagccg cgtcagacgc caggaacacc gcggcaccaa
     2581 tcaactcctc tggcttgcca aaccggccca tgggtgttcg aagcaagaac tcacgccctc
     2641 gctccgtacc atccagcaac gcggcgttca aatccgggcg cagcaccccg ggcacaatcg
     2701 catttacgca caccccaaac tttgcccact ccacggcgag agatttcgtt agcgaagcca
     2761 ccgcgccttt gctggcgcaa taggcggcaa cttcatacag tccaacaaac gacgccagcg
     2821 agccaatatt gatgatgcgt ccatggcgtc gctcgagcat gtgccttcca aagactcggc
     2881 aagcccgcat cgtgccgttg agattggttt ccatgatcac gttccaatcc gcttcggaga
     2941 catccaaagt cggctggcgc ttcgtgcggc cggcgcagtt cacgagaatt tccacgcttc
     3001 cgaatttcgt caggacgcct tgcagtaatc tttgcagcgt gccgttgtct gtcacatcgc
     3061 acgtgaccgc gagtgacttc tgtcccaacg cctccaccgc ggccgctgcg ctttgcgctt
     3121 ccgcttcacg ccgccccgtc ggaaccacgc tcgcgccggc ctgcgccaat cctttggcta
     3181 gcgcgagacc aataccgctc gtcccgccaa tcaccacggc agtcttgttt ttgagatcga
     3241 gtaagggata tcccatggat tctcgcagcc ttcgccgaac aatcgaaggg tagcgaggaa
     3301 ttataaaggg aaggcgtagg cgatccttga ccgcgagtcg cgataggtca tctatttttg
     3361 tttagtcgcc cactagagtc cggctgtgcg gcgctcttcg actgcaattc ccccgcgtac
     3421 ggtggccgag tcttgatcgg cccctccggt ccgtggacat gatcggaata ctgaaattga
     3481 acgggcacgg tgccgctccc gcccgtcagt cttacttcct gaaagacctg cgttagtgga
     3541 tttgcttcga acaccggaaa gtcgccgcat tcttctccct gcttcgaagt ccaccctaga
     3601 aaattcacgg cggcgggatc atctcccgca cggatcgcat cctccacgtc cggatcggtg
     3661 cgcgcttcat tcaactcgtg atagaaggtc gcgaccacgt tcttccagga ttggtcaaat
     3721 accggaattc cattcacagt cccgtccggc agtttttccg agaacactcc taccgcgtaa
     3781 tagag
//
LOCUS       DASTPM010000024         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_721679, whole genome shotgun sequence.
ACCESSION   DASTPM010000024 DASTPM010000000
VERSION     DASTPM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721679"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFR42_00370"
     CDS             complement(<1..236)
                     /locus_tag="VFR42_00370"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU0367632.1"
                     /translation="MSNPARCTCPRCSIRNLMGPAVVTSIGVLFLLSELGNGYFHIAH
                     TYPVLFIVMGAILLASSLASMDGHVETAVPPMPPP"
     gene            complement(258..965)
                     /locus_tag="VFR42_00375"
     CDS             complement(258..965)
                     /locus_tag="VFR42_00375"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HEU0367633.1"
                     /translation="MKCAVHTDVDATGYCRNCGKPMCSACSRPVRDVLYCEDCLATVM
                     GHGTPAPVAAPATPGFASRPAEPSPAMPAMPAYAPPPGAPYQIRPAGSSSPTIAFILG
                     LFPGLGAIYNGQYNKGLIHIAIFASIIVALSTDIGDGLGAMLGIFLGGFVFYCAFEAM
                     RTAQAKLAGTAAPSDPLESWSKERPIGPIILIGLGALLLLNNFRFFDFIRLGRFWPLI
                     LIGVGFYMFRNRLAGRQ"
     gene            complement(1006..1761)
                     /locus_tag="VFR42_00380"
     CDS             complement(1006..1761)
                     /locus_tag="VFR42_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0367634.1"
                     /translation="MNWTCEQTELLLTDYLDGLLPADEQRAFDLHVNSCARCTPLLSS
                     VMHAMSSLRALPELETPPRLVYNILDATLGPRETVSGWAAFLAWLKSLASPKFAYGAV
                     SLAATFLMFVTVSGSVNWKKPKLADLAPSNIYRNTNRRVHLAYAAGTKYVSDLRVVYE
                     IQSRLRQDNEVPTNRENATPESNPTPGRTDGSGPASPRQQNRANDVSSQLQVLAEEVP
                     LLSGKLSLVSSGRLDFMTSGKLGFVIGGKLGVR"
     gene            complement(1758..>2216)
                     /locus_tag="VFR42_00385"
     CDS             complement(1758..>2216)
                     /locus_tag="VFR42_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0367635.1"
                     /translation="TGNRTEAEDLSQDVFLRVYRTLASYRSAHGGFATWMTSVTRNPL
                     IDHYRRTKRDRVTDSLDDAMPVVENKESSGRRPDEQALLGELSAQVQAALTHLSPELR
                     EAVVLRDLQQLEYSEIQTVLAVPEGTVKSRINRGRIELARILQQMGVRPS"
BASE COUNT          477 a          672 c          641 g          426 t
ORIGIN      
        1 gggggcggca tcggcggtac ggcagtttcc acatgtccgt ccatcgaagc taacgatgat
       61 gccagcagaa tggctcccat cacgatgaac agcaccggat acgtgtgagc aatatgaaag
      121 tatccgttac ccagttcgct cagcaagaag aggacgccga tgctggtgac caccgcagga
      181 cccatcaggt tgcggatcga gcagcgcgga caagtgcagc gggccggatt gctcatcgtc
      241 gaatcttcct ccaattgcta ctgccgtccc gcaagccggt tgcgaaacat gtagaagccc
      301 acaccgatca aaatcagcgg ccagaatcgc cccaggcgga taaaatcaaa aaagcggaag
      361 ttattgagca acagcaacgc gccgagtcca atcaaaatga tcgggccaat ggggcgttcc
      421 ttgctccagg attccagagg atcagacgga gcggcagtcc cggcgagctt ggcttgcgct
      481 gttcgcatgg cttcgaaggc gcaataaaaa acaaagccgc caagaaatat tcccagcatc
      541 gcgccgagcc catctccaat atcggtggaa agcgccacga tgatcgaagc gaaaatcgcg
      601 atgtgaatca gtcccttgtt gtattggccg ttgtaaatcg ccccgagccc cgggaagagc
      661 ccaaggatga atgcgatggt ggggctgctg ctaccggcag gccgaatttg ataaggagct
      721 ccgggcggcg gagcgtacgc aggcatggct ggcattgccg gagatggctc cgcaggccgc
      781 gaagcaaatc cgggtgttgc gggcgcggcc actggggcag gcgtgccatg ccccatcacc
      841 gtcgccaggc aatcttcgca gtacaacaca tcacgcactg gccgcgagca cgccgaacac
      901 atcggcttgc cacaatttct acagtacccg gttgcgtcca catccgtatg gacagcgcac
      961 ttcatcgagt tctcccttcc aacaacgtac ggcgaggcgt ttcgctcatc gcacgccgag
     1021 cttgccgccg attacaaaac cgagctttcc gctggtcatg aaatcgagcc tgccgctcga
     1081 gacaaggctg agtttgccgc taaggagggg aacttcctcg gccagcacct ggagctgtga
     1141 agaaacgtcg ttcgcgcggt tctgctgccg cggactcgcc ggcccgcttc catccgtccg
     1201 acccggagtc ggattcgatt ccggcgtcgc gttctcgcgg ttggtgggca cttcattgtc
     1261 ttgtcgcagc cgcgactgaa tctcatacac cacgcgcaaa tcgcttacat atttggtccc
     1321 ggccgcatac gccaaatgca ctcggcgatt ggtgttgcgg tagatattgg aaggcgccag
     1381 atcggccaat ttcggctttt tccagttcac actgcccgac accgtcacga acatcaggaa
     1441 tgttgctgcc agcgaaaccg ccccgtaggc aaacttcggc gaagccaggc tcttcagcca
     1501 cgccaagaag gccgcccagc cgcttacggt ttcgcgcggg cccagagttg cgtccaaaat
     1561 gttgtaaacc aggcgcggcg gcgtttcgag ttccggcaat gctcgcaggc tgctcattgc
     1621 gtgcatcacg ctggacagca aaggcgtgca gcgcgcacac gagttcacgt gcaggtcaaa
     1681 cgcgcgctgc tcgtcggcgg gcagcagccc gtcgagatag tcggtcagca gcagttcagt
     1741 ttgttcgcag gtccaattca tgatgggcga acccccattt gttgcaaaat ccgtgccagc
     1801 tcgatgcgac cgcggttgat cctcgatttt accgtgcctt cgggcacggc gaggacagtt
     1861 tggatctcac tgtactcaag ttgctgcagg tcgcgcaaaa ccaccgcctc gcgcaattcc
     1921 ggcgaaaggt gcgtcagcgc cgcttgcacc tgcgcgctta attcgccaag caaagcctgt
     1981 tcatcagggc ggcgtcccga tgattcctta ttctccacca ccggcatggc gtcatcgagc
     2041 gagtccgtga ctcggtcgcg tttggtgcgg cggtaatggt caatcaaagg gttgcgcgtc
     2101 acgctggtca tccaagtagc aaatccgccg tgcgccgaac gatagctggc caacgtacgg
     2161 tacacccgaa ggaacacatc ctgcgagagg tcctcggctt ccgtccggtt gccggt
//
LOCUS       DASTPM010000025         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1038857, whole genome shotgun sequence.
ACCESSION   DASTPM010000025 DASTPM010000000
VERSION     DASTPM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038857"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFR42_00390"
     CDS             complement(<1..304)
                     /locus_tag="VFR42_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367636.1"
                     /translation="MGAKVDNSMRGMIGRSAAGVLAVATGAYGNGHALHAPVLVLNAS
                     YEPINVCAARRAIVLVLKGVAMAEEENGHFLHAARVAMRVPSVIRLLEYRRIPHQTR"
     gene            complement(310..1113)
                     /locus_tag="VFR42_00395"
     CDS             complement(310..1113)
                     /locus_tag="VFR42_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU0367637.1"
                     /translation="MFPADCRICGDPLIRISRLPICETCLDAILPIAGTLCDLCGKRI
                     LSPYAIAQQSADNSYEQLCGLCRRLEPPFAKAAAYGSYQGGLRELIHMLKYQQVRPAA
                     NVLGRMLAEAVTDLEPHWGPASVLVIPVPLFARKLRQRGFNQAELIARVALKGIAAKD
                     KYALSADVLERTRETQSQIGLTRHQRRENLRGAFRVKDHTKISGREVLLVDDVFTTGT
                     TASECARVLRRAGASRVWVATVARTLKAEVLGLRLEPQDEDALTMAAHG"
     gene            1295..1540
                     /locus_tag="VFR42_00400"
     CDS             1295..1540
                     /locus_tag="VFR42_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367638.1"
                     /translation="MEGSRNAATLFGLNGCFLPGFLLVGGPGGSYHLFWIEAHGSLAG
                     EQGDGHEAPRAPVTTTASRWTHTQHTQEVAFEEIART"
     gene            1865..>2635
                     /locus_tag="VFR42_00405"
     CDS             1865..>2635
                     /locus_tag="VFR42_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HEU0367639.1"
                     /translation="MSTQTAAPPEKKYRPYVPENMEMKEFTFRAVLIGLVMTVILGAA
                     NAYLGLRAGMTIAATYPAAVIGMALLRLMKGSLLEENIARTVGSIGESVAAGAVFTIP
                     AFLISGAWSSFGFHDAYWKSVALMAVGGSLGILFVTLLRRVMVEDPELPFPESVAASE
                     IHKAGQQGAGAAKLLFANMGLGGLIYLLGAFQLFNASKAFVVHIGQIGTSMLKLGRTA
                     AAPIVNVGGVTTFQAPAISPAYLGVGYIIGPRLASLNFA"
BASE COUNT          525 a          772 c          823 g          515 t
ORIGIN      
        1 cccgcgtctg gtgcgggatg cggcgatact ccagcaggcg gatgacggac ggaacgcgca
       61 tggcgacgcg ggcggcatgc aggaaatgcc cattctcttc ttcggccatg gcaacgccct
      121 tcagcacgag cacgatggcg cggcgcgcgg cacacacgtt gatgggctcg taggacgcgt
      181 tcagcaccag caccggtgca tgcagagcat gaccattgcc gtaggctccg gtagccacgg
      241 ccagcacgcc ggcagcggat ctgccgatca tgccgcgcat cgaattgtcg actttcgctc
      301 ccataaaacc tagccgtgtg ctgccatggt cagggcatct tcgtcctgtg gttcaaggcg
      361 caggcccagc acttccgcct tcagtgtgcg cgccacggtg gccacccaga cccgacttgc
      421 gcctgcacga cgcaggacac gggcgcattc ggaagcggtg gttccggtgg tgaaaacgtc
      481 gtcgaccagc aggacttcac gtccagaaat tttggtgtga tccttgaccc ggaaagcacc
      541 gcgcaaattt tcgcggcgct ggtggcgcgt taaaccaatt tgcgattgcg tctcgcgcgt
      601 tctctccagg acatccgcgc tgagcgcgta cttgtctttc gccgcaatcc ctttcaacgc
      661 cacgcgggcg atcagttccg cctgattgaa cccacgctgc cgcagtttgc gggcaaacaa
      721 cggcaccgga ataacgagta ccgaggccgg tccccagtgc ggttcgagat cagtgactgc
      781 ttccgcgagc atgcgcccaa gcacgttggc ggcggggcgc acttgctggt acttcagcat
      841 gtgaatcaac tcgcggagcc caccttggta actgccatag gcggccgcct tagcgaaggg
      901 aggctcgaga cgacggcaga gtccacagag ctgctcgtac gagttgtccg cagactgctg
      961 agcgatcgcg tacggtgata ggatgcgctt tccacagagg tcgcagagag tcccggcgat
     1021 gggcaggatg gcatccagac aggtttcgca gatgggaagg cgcgaaatac ggatcagtgg
     1081 atcgccgcaa atgcggcagt cggcgggaaa caacgtggcg aagaggcctt cagcgacttc
     1141 cgttgcccac gacagaatag caggccctct ggctacagaa ctggcctgcg tagcccgagt
     1201 tcggtcccag ctactgctga agacgacctc ctgacatccg ggaatggcga gacggatgcg
     1261 tgcccggatg agatctgctt aagtataacg ggcgttggag ggctcacgca atgcggcgac
     1321 gctctttggt cttaatgggt gtttccttcc gggcttcctg ctggtcggcg gcccgggcgg
     1381 aagctaccat ctcttctgga ttgaagcgca tgggagcctc gcgggggagc aaggcgacgg
     1441 tcacgaagcg ccacgagcgc cggtaacaac caccgcatcg cgctggacgc atacgcagca
     1501 cacccaggaa gtggcgttcg aggaaattgc gcggacgtga aacgaaaccc tcgcggccgc
     1561 cacagtgcca acaatgaaac tctctaagca gcgaagccca aaagccggag ccgaacgagg
     1621 cgcacatcgg gatcactccc ttgggctcac gaaggacaaa tggcccaaaa ccgattgacc
     1681 tgattttaac cccgtttgcc tcggagtttc aaaagctgcc ggggtagcgg gcaacggttc
     1741 agaactggac acattcctat ctggcgaggt gcacttgtat tgggcggtca cgccgtgcta
     1801 acgtgcgcgg tcgcgaccta atttggggat atatctcgtc ccacgaagta cttgcgaggc
     1861 cttcatgagc actcagacag cggctccacc ggaaaagaag taccgtcctt acgtccccga
     1921 gaacatggag atgaaggaat tcacgtttcg agccgtgctg atcgggctag taatgaccgt
     1981 tatccttggc gccgccaacg catatctcgg attacgcgcc ggcatgacga ttgcagccac
     2041 ttacccggcg gcggtcatcg ggatggccct gttgcggttg atgaagggct cgctgttgga
     2101 agagaacatt gctcgaactg tgggttcgat tggcgaatcg gtagccgcgg gtgcggtctt
     2161 cacgattccc gcatttctga tttccggcgc ctggagttcg tttggatttc acgacgcgta
     2221 ctggaagtca gttgccttga tggcggtggg cggatcgctc ggcattttgt tcgtgaccct
     2281 gcttcgacgc gtcatggtgg aagacccgga acttcccttc ccggagtctg tcgccgcatc
     2341 ggaaatccac aaagcgggac agcagggtgc gggcgctgcc aagctgctct ttgccaatat
     2401 gggcctgggc gggttgatct atctgcttgg cgcctttcag ttgttcaacg ccagcaaagc
     2461 tttcgttgtg cacatcggac agatcggcac cagcatgctg aagctgggga ggaccgcagc
     2521 ggcacccatc gtgaacgttg gcggggtcac gactttccag gctcctgcga tcagccctgc
     2581 gtatctgggc gtgggctaca tcattgggcc gcggctggca tcgctgaact tcgca
//
LOCUS       DASTPM010000026         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_116361, whole genome shotgun sequence.
ACCESSION   DASTPM010000026 DASTPM010000000
VERSION     DASTPM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116361"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VFR42_00410"
     CDS             <1..1187
                     /locus_tag="VFR42_00410"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0367640.1"
                     /translation="FGLTNGAAGLGAVALPEKRTGLGSTGNVYEELGVATVINAQGTM
                     TYLGGSLPHPEVAAVMALAAQHFVPIVELEVAAGKRICELLKLPPDYDAIVTCGAAAG
                     MQSGLAGILTGNNPKFIEQIPDLTGMKSEVIIQKSHRNPFDHQLRNTGAKLVVVETRE
                     DVKKAINPQTAMMHFSNFANASGQIKVDEWVKLAHENNLPAFIDAAADTPPVSNLWNF
                     AKMGYDLIAFSGGKAIRGPQCAGLLIGKKELVANALLNNSPHEDVIGRSQKVGKEEIV
                     GMVKALEIYLNEDHAAIEKEWWRRLDAISSEVTRVPGVTTSYHVPDVANHVPHMNIFW
                     DPRKISLSPRDAAKAMREGKPSVVIGGSENGLGMSSFMLKPGEEKIIAARLVELFKSH
                     TA"
     gene            1259..>2469
                     /locus_tag="VFR42_00415"
     CDS             1259..>2469
                     /locus_tag="VFR42_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0367641.1"
                     /translation="MPDTAYHTMPTRTSYVRWGLVFWLFVLSAVAYLDRVNLSIAGAK
                     LTEEFGISNVRLGFVFSAFLFGYALFQTPAGWLADRFGPRRVLTLGVIWWGVFSALTA
                     AISHSIVNAVLVLIGIRFLLGAGEAIMYPASNQFVARWIPTQERGVANGIIFAGVGIG
                     AGITPPLISHIMLRYGWRSSFLLSAVIGLAAGAVWFLIARDTPEAHAAVSPSELAAIQ
                     QGIPKASGAVQSSRISWLTILTSPTVWMLSLGYFCFGYVAWVFFSWFYLYLARVRGLD
                     LKASSLYATLPFLAMAVCSPLGGAISYALAKRVGPRVGRCSIAIFGFALTSIFIIFGS
                     AAQDTHVASFVLAGGAGSLYLSQSAFWAVSADLGGKSAGGLSGFMNMANQIGGMITVS
                     LTPFLAGQFGW"
BASE COUNT          452 a          676 c          769 g          572 t
ORIGIN      
        1 cttttggcct gacgaatggg gcggctgggt taggtgccgt tgcgttgccg gagaaacgga
       61 ctggccttgg ctcgacaggg aatgtctatg aagagctcgg cgtcgccacg gtcatcaatg
      121 cgcaagggac catgacgtat ctcggcggct cgttgcccca tccggaggtc gcggcggtga
      181 tggcgctggc ggcgcagcac tttgtgccca tcgttgagtt ggaagttgct gcggggaagc
      241 ggatctgcga attgctgaag ctgccgccgg actatgacgc tatcgtcacc tgcggagcgg
      301 ccgctggaat gcagtccggg ctggccggga ttctgaccgg caacaatccg aaattcatcg
      361 agcagattcc cgacctgacc ggcatgaagt cggaagtgat catccaaaaa tcgcaccgca
      421 atccattcga tcatcagctt cggaatacgg gcgcgaagct ggtcgttgtg gaaacgcgcg
      481 aagacgtgaa aaaggcgatc aatccgcaga cggcgatgat gcacttttcg aatttcgcga
      541 acgcttcggg ccagatcaag gtggacgaat gggtgaagct cgctcacgag aacaatcttc
      601 ccgcgttcat tgacgcggcg gcggacactc ctcccgtttc gaatctgtgg aacttcgcaa
      661 aaatgggcta tgacttgatc gcgttcagcg gcggcaaggc aattcgcggg ccgcaatgcg
      721 cgggcttgct catcggcaaa aaggagctcg tcgccaacgc cctgctcaac aacagtccgc
      781 atgaggatgt cattggtcgc agccagaagg tcggcaaaga agagatcgtt ggcatggtca
      841 aagcactgga aatttatttg aacgaagacc acgccgcgat tgaaaaagaa tggtggcggc
      901 ggctggacgc gatttccagc gaggttaccc gcgttcccgg tgtgacgact tcttatcacg
      961 tgccggatgt ggcgaatcat gtgccgcata tgaatatttt ctgggacccg cggaagattt
     1021 cgctttcgcc gcgcgacgct gcgaaagcca tgcgcgaagg caaaccttcc gtcgtgatcg
     1081 gagggtcgga aaatggactg ggtatgagtt cgttcatgct gaagccgggc gaagaaaaaa
     1141 tcattgccgc gcggctggtc gagctcttca agtctcatac ggcctgaagc ggagtttttt
     1201 ttcacgcagc ggcgggccat gtgccgctct gatttttttc attgcgcaag gagcaaattt
     1261 gcctgatacg gcctaccaca ctatgcccac gcggacgagt tatgtccgtt ggggcctggt
     1321 gttttggctc ttcgtgctga gcgccgtcgc ttatctcgat cgggtcaatc tttccatcgc
     1381 tggcgcaaag ctgacggaag aattcggcat cagcaatgtc cggctcggtt ttgtgttcag
     1441 cgcatttttg ttcgggtacg cactgtttca gacgccggca ggttggctcg ctgatcgttt
     1501 tggtccgcgg cgagtactca ccctgggcgt aatctggtgg ggagtgttca gcgcgctgac
     1561 cgcggcaatt tcgcacagca tcgtcaacgc agtgctggtg ctgattggca ttcgattcct
     1621 ccttggcgcg ggcgaggcca tcatgtatcc ggcgtccaac cagttcgtgg cgcgctggat
     1681 tcccacgcag gaacgcggag ttgcgaatgg gatcatcttc gcgggagtcg gcatcggcgc
     1741 cggcatcact ccgcctctga tctcgcacat catgcttcgt tacggctggc gctcctcgtt
     1801 tttgctgagt gccgtcatcg gccttgccgc gggcgcggtg tggtttctga ttgcacgcga
     1861 tacgccggag gcgcatgcag ccgtttcgcc gagcgaactg gccgcgattc aacagggaat
     1921 ccccaaagcg agcggtgcgg tacagtcttc gcgaatttcc tggctaacca tcctgaccag
     1981 ccccacggtc tggatgcttt ctctgggcta cttctgcttt ggctatgtag cgtgggtttt
     2041 cttcagctgg ttttatcttt acctggcgcg ggttcgcggc ctcgacttga aggccagctc
     2101 gctttatgcc acgctgcctt ttctggcaat ggcggtttgc agcccgctcg gcggcgcgat
     2161 cagctatgcg ctggcaaagc gcgtcggtcc gcgcgtgggg cgctgcagta ttgccatttt
     2221 cggtttcgcg ctgacctcga ttttcattat cttcggctca gcggcacagg atacgcacgt
     2281 agctagcttc gttctggccg gaggcgcagg gtcactctat ctttcgcaga gcgcgttctg
     2341 ggcggtgtcg gcggacctgg gcggcaaatc cgccggcggg ctctccggct tcatgaacat
     2401 ggccaaccag attggcggca tgatcaccgt ttcgctgacg ccgtttcttg cgggtcagtt
     2461 tggatggaa
//
LOCUS       DASTPM010000027         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_231649, whole genome shotgun sequence.
ACCESSION   DASTPM010000027 DASTPM010000000
VERSION     DASTPM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231649"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFR42_00420"
     CDS             <1..1120
                     /locus_tag="VFR42_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0367642.1"
                     /translation="PYENQIFDYDATVIENLRRAGAVLLGKASMIELAGGMNYRFASA
                     SLQGAAKNPWNTSCWTCGSSSGSGAIVAAGLAAFAIGTETWGSIICPSAYCGISGLRP
                     TFGRVSRYGAMALSYSMDKIGPMARSAEDCAHIFAVIAGHDIKDQSTLPLDKAAFTYS
                     PSAELSAKPLRVGWLTNAWKQIDPRAEKPVQAAERTVRKMFPGTRDAALPEGPFEAAG
                     GIIISAEGAAAFRDLIRSGKVAQLADPLGQINGYVGEQIEAADYVRALQVRGFLQQKM
                     ETLFDGFDVLVAASQPLGANALDANLETDLVFPDPLGGIGNLCGLPALSVPCGFTDQN
                     LPVGLQFVAKASNDLAVIRAARTFQQHTDWHRKHPKVS"
     gene            complement(1143..>3353)
                     /locus_tag="VFR42_00425"
     CDS             complement(1143..>3353)
                     /locus_tag="VFR42_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0367643.1"
                     /translation="LHSASDAALIQQKFWSAVSARNDDTVSCRCSLRAKSGQFRWFDA
                     VATNRIANPDVHGILISYQDVTEIQRMEAQRLVLSNIVHALNQTSNLDELLFEIHDAL
                     KKVVYAENFFVALYDPQTEMFHFPFFVDQFDPPPPPQKVARTCMAYIFRTGNSRMIPQ
                     SEFDRLAAEGEVELVGSASPAWLGVPLKTPNATIGVMVVQHYQNENAYDQRDLEFLDS
                     VGGHIALAIERVRAEEALRKSESMFRLLFSHTPLPMWVFDTESLRFLQVNEAATKQYG
                     FSDDEFRRMTVRDIRTDGNEPSFAKHVEEWKQEGRHHGHWRHKRKDGKSFAVDVVSHK
                     LEYGGRSVRLVVAQDVSERQLLEQQLRQAQKMEAIGRLAGGVAHDFNNLLMVIKGHTE
                     LLLDVLPVADHVTRKIEQIDRSADRATALTRQLLAFSRMQVLQPRPMNLNSVVDEMGK
                     LLPRLIGEDIELIVRVSGDLGTIRADASQMEQIIMNLAVNARDAMPNGGKLTIETQNI
                     ELDHYYREAHPIVQPGRYVLLSVSDNGTGMDAETQAHIFEPFFTTKEQGKGTGLGLAT
                     VYGVVKQSGGFVWVYSELDKGTTFKIYLPRVDETAAAIVGAPSAAQVLRGTETVLLTE
                     DEQDVREVAREFLESAGYQVLQAPSGEAALKLAAAHNGPIALLVTDMVMPGMSGQELA
                     RKMRIVRQGLRVLYMSGYSEHAAEEAAKSEGSAVLAKPFSRGALLRTAREVLQK"
BASE COUNT          674 a         1013 c          944 g          722 t
ORIGIN      
        1 gccttacgaa aatcagattt tcgattacga cgctactgtc atcgaaaatc ttcgccgcgc
       61 gggtgccgtg ctgctgggta aagcttccat gatcgaactc gccggcggca tgaattaccg
      121 tttcgcctcc gcctcgctgc agggtgcggc aaaaaatccc tggaacactt cctgctggac
      181 ttgcggttca tcctccggtt cgggggcaat cgtcgccgcc ggactggccg cctttgcaat
      241 cggcacggaa acctggggct ctatcatttg tccgtcggcc tactgtggca tcagcggact
      301 tcgccccacc ttcggccgcg tcagccgtta tggcgctatg gctctttcgt attccatgga
      361 caagatcggt ccgatggccc gctccgccga agactgcgcg catatcttcg ccgtaattgc
      421 cggccacgac atcaaagacc aaagtacgct gccgctcgac aaagctgcct ttacctattc
      481 accttctgcc gaactctccg caaaacctct gcgcgtcggc tggctcacca atgcatggaa
      541 acaaatcgac ccacgcgcgg agaagcccgt acaggcggcg gaacgtacgg tccgaaaaat
      601 gttccccggc actcgcgatg cggcgcttcc tgaaggcccg tttgaagccg cgggtggaat
      661 catcatctcc gccgaagggg ctgccgcgtt ccgtgatcta attcgctccg gaaaagtggc
      721 gcaactcgcc gacccgctcg gacaaatcaa cggctacgtc ggcgaacaaa tcgaagccgc
      781 cgattacgtc agggcgttgc aggtccgcgg atttttgcag cagaaaatgg agacactctt
      841 cgatggcttc gacgtgctgg tagccgcttc gcagccgctg ggtgcaaacg cgctcgacgc
      901 caatctcgaa acggatctag tcttccccga tcccctcggg ggtatcggca atctttgcgg
      961 cctacctgca ctttcggtac cttgcggctt taccgatcaa aatcttcctg tcggcctgca
     1021 attcgtcgcc aaagccagca acgacttggc ggtaatccgc gccgctcgca cttttcagca
     1081 gcatacagac tggcatcgca aacacccaaa ggtctcttag tgtggacgaa aggattttcg
     1141 atttacttct gcagaacttc gcgcgcggtg cgcagcaggg cgccgcggct gaacggcttg
     1201 gccagcaccg cggaaccctc gctcttggca gcttcctccg cggcgtgctc gctgtatcca
     1261 gacatataca gcacgcgcaa gccttgccgc acaatgcgca tcttgcgagc aagctcctgg
     1321 ccgctcattc ccggcatcac catgtccgtg accagtagag cgatcgggcc gttgtgcgca
     1381 gcggccagtt tcagcgcagc ttcgccgctg ggcgcctgca gcacttggta gcctgctgat
     1441 tccaaaaatt cccgcgccac ttcacgcacg tcctgctcgt cctcagtaag cagcactgtt
     1501 tccgtcccgc gcaaaacttg ggccgccgag ggagcgccaa caatcgcggc ggctgtttcg
     1561 tccactcgcg gcaggtagat cttgaacgtg gtccctttgt ccagctcgct gtacacccac
     1621 acgaatccgc cgctctgttt caccacaccg tacacggtag ccagcccgag gccggttccc
     1681 ttcccttgtt ctttggtggt gaaaaacggt tcgaaaatgt gcgcctgcgt ttccgcgtcc
     1741 atgccagtgc cgttgtcgga aacggaaaga agtacgtacc gcccgggctg cacaatcggg
     1801 tgcgcctcgc gatagtagtg atccagctcg atgttctgcg tctcgatggt cagcttgccg
     1861 ccattgggca tcgcatcccg tgcgttgacc gccagattca tgatgatctg ttccatctgg
     1921 ctggcgtccg cgcgaatcgt accgaggtca cccgaaacgc gcacgatcaa ttcgatgtct
     1981 tcgccgatca gccgcggcag cagcttgccc atttcgtcca cgacggaatt gagattcatc
     2041 ggccgcggct gaagcacttg catccggctg aacgcaagca actggcgagt cagcgccgtg
     2101 gcacggtcgg cggagcgatc aatctgttca attttccgcg tcacatgatc ggcgacggga
     2161 agcacgtcga ggagtaattc tgtgtggccc ttgatgacca tcagcagatt gttgaagtcg
     2221 tgggccacac ctcccgctag gcggccgatg gcttccattt tttgcgcctg ccgcaactgt
     2281 tgttcgagca actggcgttc actcacgtcc tgcgccacca ccagacgcac gctgcggccg
     2341 ccatattcca gcttgtgtga gacgacgtcc actgcgaaac tcttgccgtc cttgcgtttg
     2401 tgacgccaat ggccgtgatg gcggccttcc tgtttccact cttcgacgtg tttcgcgaag
     2461 gatggttcgt taccgtcggt gcgaatgtca cgcacggtca tacggcgaaa ttcgtcgtcc
     2521 gaaaaaccgt attgtttggt ggcggcttcg ttcacctgca ggaaccgcag cgactcggtg
     2581 tcaaacaccc acatgggcag cggagtgtgc gaaaagagca gccggaacat ggattcgctc
     2641 ttgcgcaagg cttcttcggc gcgcacgcgt tcgatggcaa gagcaatgtg tccgccgacg
     2701 gaatcgagaa actccaggtc acgctggtcg taagcgtttt cattctggta atgctgaacg
     2761 accattactc caatcgtagc gttcggagtc ttaagcggaa cccccaacca tgcaggtgat
     2821 gccgagccga ccaattcgac ttcaccttcc gcggcaaggc gatcgaactc cgactgagga
     2881 atcatgcgcg aattgccggt gcgaaagatg tacgccatgc aggtgcgagc gactttttgt
     2941 ggcggcgggg gcgggtcaaa ctggtccacg aaaaacggga aatggaacat ttcagtctgc
     3001 ggatcgtaaa gagccacaaa aaagttttcc gcatagacga cttttttcag cgcgtcgtga
     3061 atttcaaaga gtagctcatc gaggttggaa gtctgattca gggcgtgaac gatgttggag
     3121 agaaccagtc gctgcgcttc catgcgctga atttcggtga catcttgata agaaatgaga
     3181 atgccgtgaa catcgggatt ggcgatgcgg ttggtggcca cagcgtcgaa ccagcggaac
     3241 tggccggact tggcgcgcaa actgcagcgg caggagaccg tatcgtcgtt ccttgcggag
     3301 acggcggacc agaatttttg ctgaatcagc gctgcgtcgg aggccgagtg aag
//
LOCUS       DASTPM010000028         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1356044, whole genome shotgun sequence.
ACCESSION   DASTPM010000028 DASTPM010000000
VERSION     DASTPM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356044"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFR42_00430"
     CDS             <1..667
                     /locus_tag="VFR42_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367644.1"
                     /translation="KEADPDLLNARIYLGTAYASLYIPGAPSKENQDHGNQAIQEFKE
                     ILDKDPNNISANDQIGSILFLMAGTPFDAKKFEESKTYHQKHIQLKPDDPEPYYWIGV
                     IDWTLAFRANGEMRMDYNKSNVKKQVRDTDPLPTAIRSDYASKYGPLIEEGIADLQKA
                     TSLRPDYDDAMAYLNLLYRRKADMVESKEEREALLKQADDLVDKVKEIKQKRAEQPQT
                     PGS"
     gene            771..2984
                     /locus_tag="VFR42_00435"
     CDS             771..2984
                     /locus_tag="VFR42_00435"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU0367645.1"
                     /translation="MPSPSNLPLHFHRDRTERELDARLDELLAKLRKNRPSEDPWMVR
                     RAYEIAAERHRDQLRSSGDPYLTHLLEVAHILADMRLDATTLTAALLHDAIEDTEYPV
                     TRIEERFGPEVARLVEGVTKIGRLNMMAPEARQAENVRKMLLAMVNDVRVVMVKLADR
                     LHNMRTLDFLEPAKQQRISRETLDIYAPIAHRLGMGIVRGELEDLAFRHLEPESFLSL
                     QKEVSDKAPVHKKFLEEVQATIRSKLVENGIPAELEARVKGLYSLHRKIVRQERALDQ
                     VYDLLAVRVITDTERNCYAALGVMHHIWRPVPGRFKDYIAMARPNLYQSLHTTVLHGG
                     QPFEVQIRTQDMHRIAEEGVAAHWKYKDGKPAQEDDQRITWMRQLIEWSQELEEPGEF
                     LQTLKVDLAPVEVYAFTPKGRVLELPRGATPVDFAYLVHTEVGHQCVGAKVNGQMVPL
                     RHEIVSGDVVEILTQKGHGPNRDWLSFAKSSHARSKIRHWINLQEREEAMQMGQRLLE
                     KDARHFGRSLKKIPEEDLLRVASDSGLSKIEDLYAAVGFGKYSARQILTRVLGDAAKT
                     AEQPGEDAKPTLVKTVKRMLGFGEAPLIVKGYDDLLVYRAKCCNPIPGDDIVGYITRG
                     RGVAVHTRGCPNVQNLLYQTERRIAVEWGGAGAATFQVQLMIRARDRAGLLAEITAVI
                     SGAGSNIHSLESRPDRSNARIDASLEIADRRQLETILANIRKVSGVFGVERVYQP"
     gene            3077..3685
                     /locus_tag="VFR42_00440"
     CDS             3077..3685
                     /locus_tag="VFR42_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367646.1"
                     /translation="MKLRVVLPGLCALLLAGCHQHPLTDYRPLDQAGMWSSGLEDLKK
                     LDTSDSEVAQLVKVKQARLSDDACVALVAAAHERKHPFVSGDSTANLAAAGYTEDQIL
                     EIAKADQLDAISGDAVALRLIGLSDGFVQFVLKRRLQKIPTLDTPQIARLKNTGLTEA
                     QIMEKIKAGMTDAQADREVTVREANRNHAGTGFVRVHGRKPR"
BASE COUNT          831 a         1172 c         1058 g          700 t
ORIGIN      
        1 caaagaagcg gaccccgatc tgttgaatgc gcgcatttac ctgggcaccg cgtacgccag
       61 cttgtacatt cccggcgcgc cctccaaaga aaatcaggat cacggaaatc aggccatcca
      121 ggagtttaag gaaatactgg acaaggatcc caacaatatt tcggccaacg accagatcgg
      181 gtccattctt ttcttgatgg ccggaacacc ctttgacgcc aagaagtttg aagagtccaa
      241 gacctatcac cagaagcata ttcagctgaa gcccgacgat ccggagccgt attactggat
      301 cggcgtgatc gattggacct tggcgtttcg tgccaacggc gaaatgcgta tggactacaa
      361 caagagcaac gtcaaaaagc aggtcaggga caccgatcct cttcccaccg ccatccgctc
      421 cgactatgct tccaagtatg gcccgctcat cgaagaagga atcgccgacc tgcaaaaagc
      481 cacttcactt cgccccgatt acgacgacgc catggcctat ctgaacctgc tgtaccgccg
      541 caaggccgat atggtggaaa gcaaggaaga gcgtgaggcg ttgttgaagc aagccgacga
      601 cctggtcgac aaggtcaagg aaatcaagca gaagcgcgcc gagcagccac aaacccccgg
      661 atcgtagtcg ccagttcaaa ctaagcgaaa gggcgtcccc cgagtacgcc ctttttttgc
      721 gctagccgcc ccagcctcgc gccgtaacct cttctggcgt aaactagcgt atgccgtcgc
      781 ccagcaatct acctctccat tttcatcgcg atcgcaccga gcgcgagctg gatgcgcgcc
      841 tcgatgagtt gctggccaag ctgcgcaaga atcgcccttc cgaagatccc tggatggtgc
      901 gccgcgcgta cgaaatcgcc gcggaacgcc atcgcgacca gttgcgcagt tccggcgatc
      961 cctacctcac gcatttgctg gaggttgcgc atattctggc cgacatgcgc ctcgacgcca
     1021 ccacgttgac cgcggcgttg ctgcacgacg ccatcgaaga caccgagtat ccggtcactc
     1081 gcatcgaaga gcgcttcggg ccggaagtgg cgcgcctggt ggaaggggtc accaagattg
     1141 gccgcctcaa catgatggcg ccggaagcgc gccaggcgga aaacgtccgc aaaatgctgc
     1201 tggccatggt taacgacgtc cgcgtggtga tggtcaagct ggccgatcgc ctgcacaata
     1261 tgcgcacgct ggactttctg gaaccggcca agcaacagcg catctctcgc gaaactctag
     1321 acatttatgc ccccatcgcg catcgcctgg gcatgggtat cgtgcgcggc gagctggaag
     1381 atctggcctt ccgccatctc gaacccgaat cgttcttgtc tctgcaaaag gaagtcagcg
     1441 acaaagctcc ggtgcacaag aaatttttgg aggaagtgca agccaccatc cgctccaagc
     1501 tggtggagaa cggaattccc gcagagctgg aagcgcgcgt caaaggcttg tactcgctgc
     1561 accgcaaaat cgtgcgccag gagcgcgcgc tcgaccaggt ctacgatctt ctcgccgtcc
     1621 gcgtcatcac cgacaccgag cggaattgct acgccgctct gggcgtaatg caccacatct
     1681 ggcgcccggt acccggccgc ttcaaagatt acatcgccat ggcccgcccc aatttgtatc
     1741 agtcgctgca caccactgtg ctgcacggcg gccagccctt cgaggtgcag attcgcaccc
     1801 aggatatgca tcgcatcgcc gaagaaggcg tagccgcaca ctggaaatat aaagatggca
     1861 agccggccca ggaagacgac cagcgcatca cctggatgcg gcaattgatc gagtggtcgc
     1921 aggaactgga agagcccggc gaatttttgc agacgctgaa agtggatctc gcgccggtcg
     1981 aggtctacgc tttcacgccc aagggccggg tgctggaatt gccgcgcggg gccactccgg
     2041 tcgattttgc gtacctggtg cacaccgagg tcggccatca atgtgtcggc gccaaagtta
     2101 atggacagat ggtgccgctg cgccacgaaa tcgtcagcgg cgacgtggtc gagatcctca
     2161 cccagaaggg tcacggcccc aaccgcgact ggctcagctt cgcgaaatcc tcgcatgcgc
     2221 gcagcaaaat ccgccactgg atcaatcttc aggagcgcga agaagccatg cagatgggcc
     2281 agcggctgct ggaaaaagat gcgcgccact ttggccgcag cctgaagaaa atcccggaag
     2341 aagacttgct gcgcgtggcc tccgattccg gcctctccaa aattgaagac ctgtacgcag
     2401 cggtaggctt cggaaaatat tccgcgcgcc agattctcac gcgcgttctg ggcgatgcgg
     2461 ccaagaccgc cgagcaacct ggcgaggatg ccaaacccac gctggtcaaa accgtcaagc
     2521 gcatgctcgg ttttggcgaa gcgccgctga tcgtcaaagg ttatgacgac ctgctggttt
     2581 accgcgccaa atgctgcaat cccattcctg gcgacgacat tgtcggctac atcacgcgcg
     2641 ggcggggtgt cgcagtccat acccgcggct gccccaacgt ccagaatctt ctttatcaga
     2701 ccgaacgccg catcgccgtt gaatggggcg gcgcaggagc cgccactttc caggtgcagc
     2761 tgatgattcg cgcgcgcgat cgggctggtt tgctggccga aattaccgcc gtaatcagcg
     2821 gggccggctc caacattcac agcctggaaa gccgcccaga ccgttccaac gcgcgtatcg
     2881 atgccagcct ggaaatcgcc gatcgccgcc agttggagac cattctcgcc aacatccgca
     2941 aggtttccgg cgttttcggc gtcgagcgcg tataccagcc ctgatttttc tcgtttgggt
     3001 ttacctgtcc tttcgcacaa gtacccccga cggcacacgc ccacctgtgg cccttgccag
     3061 ccgctaaaat aggagcatga agctccgcgt cgtgcttcct ggtctctgcg ccctattgct
     3121 cgccggatgc caccagcatc cgctaacgga ctatcgaccg ctcgatcagg ctggcatgtg
     3181 gtccagcggc ctggaagatc tgaagaagct ggatacctcc gattccgaag tggcgcaact
     3241 ggtcaaggtc aagcaagccc gcttgagcga tgatgcctgc gtggcgctgg tggccgcggc
     3301 gcacgagcgc aagcacccat tcgtgagcgg cgattcgacc gccaacctgg ccgccgccgg
     3361 ctatacggaa gatcaaattc tggaaatcgc caaagcggat cagctcgacg ccatcagcgg
     3421 ggacgcggtc gcgcttcgcc tgattggcct ttccgacgga ttcgtgcagt ttgttctgaa
     3481 gcgccgcttg caaaagattc ccacgctgga tacgccgcag atcgcacggc tgaaaaatac
     3541 cggactcacc gaagcccaga tcatggaaaa gatcaaagct gggatgaccg atgcccaggc
     3601 cgaccgcgaa gttacggttc gcgaagccaa tcgcaatcac gccgggaccg gttttgtccg
     3661 cgtccacggg cgtaagcccc gctgattggc aattcacgca atagtgtcat ggtatttttg
     3721 cttgcgggca tttccctcgc tacaatcgta gcgctttgaa a
//
LOCUS       DASTPM010000029         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_462368, whole genome shotgun sequence.
ACCESSION   DASTPM010000029 DASTPM010000000
VERSION     DASTPM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462368"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFR42_00445"
     CDS             <1..327
                     /locus_tag="VFR42_00445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0367647.1"
                     /translation="INDTHGHLVGSHALCRLADILRLHCREIDVAARYGGDEFVLVLP
                     EASVQAALHVAQRISSRLQNDGLEPALSVSTGVAAYPADGATLEELLSAADRALYANK
                     PTAVPK"
     gene            complement(332..1390)
                     /locus_tag="VFR42_00450"
     CDS             complement(332..1390)
                     /locus_tag="VFR42_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0367648.1"
                     /translation="MFSALGDLANGEKNVGVVAGVDLGGTAVNYTLVDDEEKFLIEGL
                     CEHPSLARQGPEICLQQIADGLKIATDKAGVALEDVVALGLDTPGPASAGGVMSARGS
                     TNFVHANWAGFDIREGLAHKLGKPVSYLNDGNAAALWGHYAIFGANSKETSISTIIGT
                     GNGGGVILEGNVVKGKNGFGGELGHVLLPYQSIAGIEDMVPKCNCGRVGDLESLCSLG
                     GIERNILPYLLKRNPGHELGGIEANAAAKKVRGMADRGDALCKEVFRIQAHAAGLFFD
                     EMINTFDPDALIVGGGALEASAGFQKWFMDEIRVGMPDQREEQAGIPIHVIPNGDTAG
                     ARGAAIEALKLARQSGLM"
     gene            complement(1785..2132)
                     /locus_tag="VFR42_00455"
     CDS             complement(1785..2132)
                     /locus_tag="VFR42_00455"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0367649.1"
                     /translation="MSRIPKLPSGRSTEASAVLFAALGDQQRLWLVSRLCDGGPRSIT
                     QLASGARISRQAVTKHLRVMERAGLVRSARRGRERMWQLEQRRLEEARRYLSQISDQW
                     DAVLERLRVFVEA"
     gene            complement(2116..2604)
                     /locus_tag="VFR42_00460"
     CDS             complement(2116..2604)
                     /locus_tag="VFR42_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0367650.1"
                     /translation="MNTDRIEKKILLRATRARVWRALSDSSEFGTWFGMKFDGPFRPG
                     ALMHGMITGTKVNAEVARLQKPHEGKKFDITIEQMEPERLFSFRWHPNAVDENVDYSA
                     EPTTLIAFTLKETRDGVLLTVTESGFDQIPPARRATAFEANEKGWGLMIKLVEEYLVQ
                     NP"
     gene            2763..3077
                     /locus_tag="VFR42_00465"
     CDS             2763..3077
                     /locus_tag="VFR42_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367651.1"
                     /translation="MIARVWHGWTNPDDADHYESHLRPELLPGISKMKGFRGSYLLRR
                     QAGEEVEFVTILLCDSIEGIKALAGPDYETAVIPEERTKYLKRFDAKAAHYEVVEEVR
                     KG"
     gene            complement(3128..>3523)
                     /locus_tag="VFR42_00470"
     CDS             complement(3128..>3523)
                     /locus_tag="VFR42_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0367652.1"
                     /translation="FCARHARNCNRRVTGLAKEALTCLTQYDWPGNVRELENALERAV
                     VIGSSDTILAEDLPDNILDSAKSAPALPVRYQDAIRNLKKQLILGSLEQTSGNITEAA
                     KLLGVHANYLHRLMRNLHLRPAAKKQAAE"
BASE COUNT          734 a         1117 c          905 g          767 t
ORIGIN      
        1 atcaacgaca cgcacggcca tctcgtaggc agccacgctc tgtgccgcct cgccgatatt
       61 ttgcgcctgc attgtcgaga gattgatgtg gccgcccgtt acggcggcga tgaatttgtc
      121 ctcgtactgc ccgaagccag cgtacaagcc gcgctccacg tcgcacagcg catctcttct
      181 cggctacaaa atgatggatt ggagccggcg ctatcggtaa gtaccggagt cgcagcctat
      241 cccgccgatg gcgcgactct cgaagagttg ctttctgcag ccgatcgcgc cctctacgcc
      301 aacaaaccta ccgccgttcc caagtaaggc ttcacatcag cccgctctgc cgagccagct
      361 tcaaagcctc aatcgccgcg ccccgcgctc ccgccgtgtc gccattgggg atcacatgaa
      421 tcggaatccc cgcctgttct tcccgctgat ccggcatgcc cacgcgaatc tcgtccataa
      481 accatttctg aaacccagcg ctcgcttcca atgctccgcc gcccacgatc agagcatcgg
      541 gatcaaaagt gttgatcatt tcgtcgaaaa acagtccggc cgcatgcgcc tgtatgcgga
      601 agacttcctt gcacagcgca tcgcctcggt ccgccatccc gcgcaccttc tttgccgcag
      661 cattcgcctc aattcctccc agttcgtgcc ccggattcct cttcaacaaa tacggcagga
      721 tatttcgctc aatccctccc agcgaacaca gcgactccag atctcccact cgtccgcaat
      781 tgcacttcgg caccatgtct tcaattcccg caatgctttg atagggcagc aacacatgcc
      841 ccaattcgcc gccaaatcca ttcttgccct ttaccacatt cccttccaga atgacgccgc
      901 cgccatttcc cgttccgata atcgtcgaaa tcgaagtctc tttgctgttc gctccaaaaa
      961 tcgcgtagtg cccccaaagc gccgcagcat ttccgtcatt caaataggaa accggcttgc
     1021 ccagtttgtg cgccagccct tcgcgaatat cgaatcccgc ccagttcgca tgcacaaaat
     1081 ttgtcgaacc ccgcgcgctc atcactccgc ccgcgctggc cgggccggga gtgtccaatc
     1141 ccaacgcgac cacgtcctcc agagcgactc ccgctttgtc cgtcgcgatc ttcaatccgt
     1201 cggcgatctg ttgcaggcaa atttccggcc cctgccttgc cagcgacgga tgctcgcaca
     1261 acccttcgat caggaacttc tcctcgtcgt ccaccagcgt gtagttcacc gccgtgccgc
     1321 ccaaatccac tcccgccact acgcccacgt ttttctctcc attggccaga tcgcccagcg
     1381 cgctgaacac ccaagcgcag cagcatcata aactaactcg ggtattttct gttcggaagg
     1441 ctgatacaag aatgtggcga acgcgccgtg gctttcggaa ctccggacga aggccaatac
     1501 gactgcgaag tgaggctagc ggacattgcc cttcgcgaag atcaatccgc tgtcttgatc
     1561 ggattgtagt ctttgcactt gcagccatcc aggcggcacg gactgcgctt gatttttttg
     1621 gtcgagagcc gttgagggta gtgaatggac ttggggtggc cgcaacggca aggttctctt
     1681 ttcatggcag tagcatagcc cgaccactag ccagaaatta agagaaatcg cgggccggcc
     1741 gcatcgcaga aaaactccgc cgtcgggcgc actcgcgaat tccgctacgc ctccacaaaa
     1801 actcgcagcc tctccagaac cgcgtcccac tgatcagaaa tctggctcag atagcggcgt
     1861 gcctcctcca accgccgctg ctccaattgc cacatccgct ctctcccgcg ccgcgcgctg
     1921 cgcaccagcc cggcgcgctc catcactctg aggtgcttgg tcacagcctg ccggctaatt
     1981 cgagctccgc tagccagctg cgttatcgat cgcggtccgc cgtcacacaa gcgggagacc
     2041 agccacaaac gttgctggtc ccccagcgct gcgaataaga ctgcagaagc ctcagtcgac
     2101 ctaccgctcg gtaatttagg gattctggac aaggtattcc tcgaccagct tgatcatcag
     2161 tccccatccc ttttcgttgg cttcgaatgc cgtagcccgt cgtgccggcg ggatttgatc
     2221 aaagcccgat tccgtaacgg tcagcagaac tccgtcacgc gtttctttca gtgtgaaagc
     2281 gatcaatgtc gtcggttctg cgctgtagtc aacattctca tctacggcgt taggatgcca
     2341 gcgaaacgaa aacaaccgtt ccggctccat ctgctcgatt gtgatgtcga atttcttgcc
     2401 ctcgtgcggc ttctgcagtc gggccacttc ggcattaacc ttggttccgg tgatcatccc
     2461 atgcataagg gcgcccggcc ggaacggccc gtcaaacttc atgccgaacc aggtgccaaa
     2521 ctcgctggaa tcgctcaatg ctcgccacac gcgcgcgcgt gtagcgcgca gcaggatttt
     2581 cttttcaatg cggtcggtat tcatgcaacc tcctggttgc agattagcag aagttcgcgg
     2641 tgacatgcaa ccatccggtt gccataacaa ccagcgggaa cccaggcttc ccgctaccgt
     2701 accgggtggc caccgttttt caggcgcgct aacctgaaaa tgcattccta tcaggaggac
     2761 aaatgattgc gagggtatgg cacggctgga caaatcccga cgacgcggac cactatgaat
     2821 cccatcttcg gccggagctg ctgccgggta taagcaagat gaaagggttt cgagggagct
     2881 atttattgcg ccgccaggca ggcgaagaag tggagttcgt gacgatattg ctttgcgatt
     2941 ccatcgaagg gatcaaggca ttagccgggc cggattatga aaccgcggta attccggaag
     3001 agcgaacgaa atatctgaag cgatttgatg cgaaagcagc ccactacgag gtggtagagg
     3061 aagttagaaa gggttgaaat ggcgcaatct acgcgcgcat ttgcaccaca ggaataggac
     3121 gtaatgctca ctcggccgcc tgcttcttag ctgccggccg gaggtgcaga ttgcgcataa
     3181 gccggtgaag gtaattcgcg tgtacaccga ggagttttgc ggcttcggtg atgttgccac
     3241 tggtttgttc cagtgaaccg aggatcaact gtttcttgag gttgcggatg gcgtcctgat
     3301 acctgaccgg cagggccggc gcggacttgg cgctgtcaag gatgttgtct ggcaaatcct
     3361 ctgccaggat cgtgtcggac gagccgataa ccacggcgcg ttccagtgcg ttttcgagtt
     3421 cgcgaacgtt tccgggccaa tcgtactgcg tcaaacatgt tagtgcctcc ttggcaagcc
     3481 cggtaacgcg gcggttacaa tttctggcgt gtcgcgcgca aaa
//
LOCUS       DASTPM010000030         4005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_548908, whole genome shotgun sequence.
ACCESSION   DASTPM010000030 DASTPM010000000
VERSION     DASTPM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548908"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..3106
                     /locus_tag="VFR42_00475"
     CDS             239..3106
                     /locus_tag="VFR42_00475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0367653.1"
                     /translation="MFLLSSIVFMAYSAAQTTTGSLRGQVMDPSGAAVAGATVIVLPA
                     QGAAITTTTNRDGSYEAKTLLPGKYTVQVFATGFAQFEAKDISVEAGAAAVLNVHLNI
                     EVQKEKVEVSENAATLDTSGASNANSITLTGKDLEALSDDPDEMQDELTALAGPSAGP
                     NGGQIYIDGFTAGQLPPKSSIREIRINQNPFSAEYDQLGYGRIEVLTKPGTDKFHGQF
                     MVHGTSDSFNAKDPFLGAAEQPSYHSILYNGNIGGPLSKKASFFFNIDRRNTNDISII
                     NTPALDSNFDPIQFTQGVENPRTRTNLTPRLDYQLTPSNTLTVRYQYYRENEQNDGIG
                     QSSLPSQAYNSLDTEHTVQISDTQTLGEHVINETRFQYVRSNATQTVLDLTPSINVSG
                     AFTGGGNTLGNLLQTTGGYEIQNYTSIAKGKHFIKFGVRARIDRDSTDKKLGFNGAFT
                     FDSLTAYQITEQGLAAGETPDQIRVAGGGAVQFTQTVGNSFVQDNYADLGFYVQDEWH
                     IRPSITASYGLRYEWQNQLSNDGGLAPRLGIAWGLGKNKQGTPKAVLRAGFGIFYNRL
                     KQQYIEQALLLNGVTQQSFVVINPDFFPNLPTPAELAAAQTSPTVYQIAPDWKTPYII
                     QSGITLEKQVTRNANLAFTYLNSRGVHALMTRNINAPLPPTFDPSNRPLGGDDNIYQY
                     DTSGDFKQNQFIVNGNVRMGSKLSLFGYYTLNYARSNTSGVTSFPSNQYDLALDYGRT
                     AFDIRNRLFLGGTISLPYMFRLSPFLVASSGVPFNVTSGVDANNDSVFTDRPTYAQFQ
                     AALANAEALGTVRSGVSFDCKPPAGQAEIPIYCGQSPARFALNLRLSKTFGFGKKAQT
                     NAAAGGPQGGGTFGRGPGGPGGGRGNRGGGMFGGGDSSGQRYSLTLAVSARNIFNNVN
                     VGTPIGVITSPIFGQSISLPVGPGSGLYSNRRIELQATFAF"
     gene            3235..3906
                     /locus_tag="VFR42_00480"
     CDS             3235..3906
                     /locus_tag="VFR42_00480"
                     /inference="COORDINATES: protein motif:HMM:NF019542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367654.1"
                     /translation="MSKQQSMGKCNLCGEMAGKASMTRHLQTCSPPEASRLSPASRKL
                     TPGPNFHLAIEGRDAKSYWMHVAVPLTAPLSRLDDFLRHTWLECCGHLSAFEVGGKRY
                     TSEPMDEEMGMRARLSEVLEVGMKFFYEYDYGSPTALVLKVVALREQGLPKGAVQLLA
                     RNEAPQVSCQRCSIQPATQICTECAWNGEGWLCEACAVAHECGDEMCLPVVNSPRAGV
                     CGYTG"
BASE COUNT          878 a         1196 c         1079 g          852 t
ORIGIN      
        1 gtgatggtgg ttagcacgca gggaaccgag aaagatgcgc cggcggtggt tacgctgttg
       61 gcgggagtcg aggccatcct gcgggcttca cccaaaggcg gtcaggacat gatcctttct
      121 ccgtggacgt tggggggcgg agaaccctcc ggcaattgaa ctaatcaagc gtgccgtgac
      181 gacataactc acaggagatg gaatgaattc gttccgtaga gccgctgcaa agtgtttcgt
      241 gtttcttctg tcctcgattg ttttcatggc gtattcggcg gcacagacca cgacgggttc
      301 gttgcgcggc caagtaatgg atccttccgg cgctgcagtt gctggtgcca cggtaatcgt
      361 tttgcccgcg caaggagcgg cgatcaccac cacgaccaat cgagatggtt cctacgaagc
      421 gaagacgctc ttgccgggca aatatactgt tcaggttttc gctactggat tcgcacaatt
      481 cgaagcgaag gacatatccg tcgaggctgg cgcggccgcg gtgctgaacg ttcacctgaa
      541 cattgaggtg caaaaggaaa aggtcgaagt ttcagagaac gcagctacgc tggacactag
      601 cggcgccagc aacgcgaatt cgattacgct gaccgggaag gacctggaag ccctttccga
      661 cgatcccgac gaaatgcagg acgaactcac cgcgttggct gggccttcgg cgggtccgaa
      721 cggcgggcaa atttacattg atggattcac cgccgggcaa ttgccgccga agtcctccat
      781 tcgcgaaatc cgcatcaacc agaatccttt ttcggcggaa tatgaccaac tgggttatgg
      841 tcgaattgaa gttcttacca agcccggtac cgacaaattt cacggccagt tcatggttca
      901 cggcaccagc gactccttca acgccaagga tcctttcctc ggagcggccg aacagcccag
      961 ctatcactcc attctgtaca atggcaacat cggcggcccg ctcagcaaga aggcatcctt
     1021 tttcttcaat atcgatcgcc gcaacaccaa cgatatctcc atcatcaata cgcctgctct
     1081 cgacagcaat ttcgatccca ttcagtttac gcagggcgtc gagaatccgc gcactcgcac
     1141 caatctcacg ccgcgcctcg attaccagct gacgccctca aatactctga ccgttcgcta
     1201 tcagtattac cgcgaaaacg aacagaacga cggcatcggc caatccagcc tcccgtcgca
     1261 agcatacaat tcgctggaca ccgaacacac cgtgcagatc agcgacacgc agacgctcgg
     1321 ggagcacgtc atcaatgaaa ctcgtttcca gtacgttcgc tccaacgcca cacagaccgt
     1381 cttggattta actccctcca ttaacgtgag cggggcgttc accgggggcg gcaatactct
     1441 cggtaacttg ctgcaaacca ctggcggata cgaaatccag aactacacct cgattgcgaa
     1501 gggaaaacac ttcattaagt ttggcgtgcg agcccgcatt gaccgcgatt cgacggataa
     1561 aaagctaggc ttcaacggcg cgttcacctt cgattccctc acggcgtacc agatcaccga
     1621 gcagggtctg gctgccggcg agacgcccga ccagatccgc gtcgctggcg gcggtgccgt
     1681 tcagttcacg caaacggtcg gcaactcgtt tgtgcaggat aactacgccg atctcggttt
     1741 ctacgtgcaa gacgagtggc acatccgacc gagcattacc gcaagctacg gcctgcgcta
     1801 cgaatggcag aatcagctca gcaacgatgg tgggctcgcc ccgcgtcttg ggattgcctg
     1861 gggtctcggc aagaataagc agggcactcc gaaagccgtg ctgcgcgctg gtttcggcat
     1921 tttctacaac cgcctcaagc agcagtacat cgaacaagcg cttttgctca acggcgtaac
     1981 ccaacaaagc ttcgttgtga tcaatcccga tttcttcccg aacctgccga caccggccga
     2041 actcgccgcg gcgcaaactt cgcccacggt ttatcagatc gctcccgact ggaaaacgcc
     2101 gtacatcatt cagtccggca tcaccctcga aaagcaagtg acccgcaatg cgaatctcgc
     2161 cttcacctac ttgaattcgc gcggtgtcca cgcgctcatg acgcgcaata tcaatgcgcc
     2221 actgccgcca acgttcgatc cttcgaaccg cccgctcggc ggcgacgaca acatctatca
     2281 gtacgacacc tccggcgact tcaagcagaa ccagttcatc gtcaacggca acgtccgcat
     2341 gggcagcaaa ttgtccctct tcggctacta cacgctgaac tatgcgcgca gcaacacgtc
     2401 cggcgtgacc agcttcccgt caaatcaata tgacctcgcg ctggactatg gacgcaccgc
     2461 ttttgatatt cgcaaccgtt tgtttcttgg tggcaccatc agtttaccgt acatgtttcg
     2521 tttgagcccg ttcttggtag ccagctccgg cgttccgttc aacgtcacca gcggcgtgga
     2581 tgccaacaat gactccgtgt ttaccgaccg gccgacctac gcgcagttcc aagccgcgct
     2641 agcaaatgca gaagccctcg gaacggttcg cagcggcgtc agcttcgatt gcaagccgcc
     2701 ggcgggtcag gcggaaattc ctatttactg cggccagagt cctgcgcgct tcgcactcaa
     2761 cctgcgcctg agcaagacct tcggtttcgg caaaaaagcc cagaccaacg cggcagcagg
     2821 aggtccgcag ggtggtggca cgtttggccg cggacccggc ggtccgggcg gcggacgcgg
     2881 caatcgcggc ggtggaatgt ttggcggtgg cgacagttcg ggccagcgtt atagcctgac
     2941 gctggcggtt tccgcgcgaa atattttcaa caacgtcaat gtaggcacac cgatcggcgt
     3001 catcacctca ccgatctttg gccagtccat ctctctgccg gtcggccccg gcagcggttt
     3061 gtactccaac cgccgcatcg aactacaggc aacctttgcc ttctagcttt gctctttgct
     3121 cgctggtcag aactacttgg ggtgcggttc gcatcagcca ggtcctccta tgctgcagga
     3181 acacagcgaa ataatagtgt ttctatttat gacgcattac aatattgctc ggccatgagc
     3241 aaacagcaat cgatgggaaa gtgtaatctc tgcggagaga tggccggtaa ggcttctatg
     3301 acccggcacc tgcagacctg cagtccaccg gaagcgtccc ggctatcgcc ggcttctaga
     3361 aaactaacac ctggcccgaa cttccattta gccatcgaag gacgtgacgc caagtcgtac
     3421 tggatgcatg tggcggtgcc gctgaccgcc ccgctgagta ggctcgacga ttttctccgc
     3481 catacctggc ttgaatgctg cggccacttg agcgcttttg aggttggcgg caagcgttat
     3541 acttccgagc ccatggatga agaaatgggc atgagagcac ggctgagcga agtgcttgag
     3601 gtagggatga agttctttta tgagtatgac tacggttctc caacggctct tgtgctaaag
     3661 gttgtcgccc ttcgtgagca aggcttgcca aaaggtgccg tacaactatt ggccagaaac
     3721 gaggcaccgc aggtaagctg ccagcggtgt agcatccagc ccgccaccca aatctgtacc
     3781 gaatgtgcct ggaacgggga gggctggctt tgtgaagcct gcgccgttgc acacgaatgt
     3841 ggcgacgaga tgtgcctgcc ggtagttaac tctcctcgcg ctggagtgtg cgggtatacg
     3901 ggttaggcgc ttttcttcca ttactactcg gaatttcgtc gcgctccact ggcttagctg
     3961 ctagtccaga tccttctctc tctttttgcg cgaggatttg agctc
//
LOCUS       DASTPM010000031         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_779626, whole genome shotgun sequence.
ACCESSION   DASTPM010000031 DASTPM010000000
VERSION     DASTPM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779626"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2632
                     /locus_tag="VFR42_00485"
     CDS             <1..2632
                     /locus_tag="VFR42_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0367655.1"
                     /translation="ATNRFRLLLRLLPAEFRGDYGSEMEQVFVAQHEEATREEGKMGI
                     WRLWWETAKGIFTTAPREHLSMLQQDSSFALRMMRKNLGFTIAAIIVLGLGIGANTAI
                     FSVVNAVLLKPLPYEHGDRLVILRERLSRNAPAGRNVSVPEMLDYRRQNRSLDAIVEY
                     HNMQFILLGRAEPAQVETGVVSWNFFDVFGAKPLIGRNFAPDDEKEGAPAVLLLSYEF
                     WLRSFGGDPTVVGKVFRMNDKPHTVIGVLPPFPQYPHENDVYMPSSACPFRTSAHMLN
                     NRQMRMLRLFGRMKPGVKPSEAEADLTTVAATMQKEFPNDYPDDAQPGVEATPLKSEL
                     THDARPTMLFLLAAAGFVLLIACANVANLNLARMVRRERELGVRTAMGASRIRLFRQL
                     LTESSLLALIGGALGLFLADSGLQLLSAYVARFTPRAREVHIDLTVLLFTLGVALFVS
                     VLTGTAPAVARRENLITALKEGGNQSTIGFVRGRLRSTLIVAQVAVSFLLLIGAGLTV
                     RSLINLQRVDPGFRPENVLTIQFSLDFSRYTSNEKTLAFWNSLLQKARSLPGVMSVAA
                     AGDFPLDKSPAFSNEFLIEGRTINPTDAKPTAELNVITPEYLSVLGIPLYSGRNFDAH
                     DQPTSPHVALINQSTAQRFWHNEDPVGKRILFQNIGGGTNWTQIVGVIGDVHEHGLDV
                     PVNDFIYLPFAQYPQTGPALMARTQGDPMVVARAVVQRLYEVDPNQPAGRIQSLEQYR
                     ADSIAAPRLTANLLGLFALLALAIAAAGIGGVMALAVSQRTHEIGVRMAIGARPAEIV
                     RMILSQGMGLALLGVVIGVATAFALTRAVKSLLFGVTPNDPATFLGVAAVLATAALAA
                     CYLPARRAARVDPLRALRTE"
     gene            2898..>3164
                     /locus_tag="VFR42_00490"
     CDS             2898..>3164
                     /locus_tag="VFR42_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367656.1"
                     /translation="MNVNERSARVCVSPAVPRLTALSIAALLFAVTAGAQSPANPRNT
                     SVALTKFVAATNHLSPEVRRHLSRGMQQYLRYAGSVANAVASPAT"
BASE COUNT          576 a         1217 c          742 g          629 t
ORIGIN      
        1 cgccaccaac cgcttccgcc ttctcctccg cctcctcccc gcggaattcc gcggcgacta
       61 cggctccgaa atggagcaag tcttcgtcgc tcaacacgaa gaagccacgc gtgaagaggg
      121 caagatgggc atctggcgcc tctggtggga aaccgccaaa ggcatcttca ccactgctcc
      181 gcgcgagcat ctctccatgc tccagcagga ttccagtttt gccctgcgca tgatgcgcaa
      241 aaatctcggc ttcaccatcg ccgccatcat cgttctaggc ctcggtatcg gcgccaacac
      301 cgcaatcttc agtgtggtca acgccgtcct cctcaagccc ctcccctacg agcacggcga
      361 ccgcctcgtc atccttcgcg agcgcctctc ccgcaacgct cccgccgggc gcaatgtctc
      421 tgtccccgaa atgctcgatt accgccgcca gaatcgcagc ctggacgcca tcgtcgaata
      481 tcacaacatg caattcattc tgctggggcg cgccgaaccc gcacaggtcg aaaccggcgt
      541 agtttcctgg aattttttcg atgtcttcgg tgccaagcct cttatcggcc gcaacttcgc
      601 gcccgacgat gaaaaagaag gcgcgcccgc cgtccttctt ctcagctacg aattctggct
      661 gcgcagcttt ggcggcgatc ccaccgttgt cggcaaagtt ttccgaatga acgacaaacc
      721 gcacaccgtc attggcgtgc ttcccccatt tcctcagtac ccgcacgaaa acgacgttta
      781 catgccatct tcggcgtgcc ccttccgcac cagcgcgcac atgcttaata accgccagat
      841 gcgcatgctg cgcctctttg gacgcatgaa gcccggcgtg aaacccagcg aagcggaagc
      901 cgacctcacc accgtcgccg ccaccatgca gaaagaattc ccgaacgatt atccagacga
      961 cgcgcagccc ggcgtggaag ccacccctct caaatccgaa ctcacccatg acgcccggcc
     1021 caccatgctc ttccttctcg ccgccgcggg gttcgttctc ctcatcgcct gcgccaacgt
     1081 cgccaatctc aatctcgccc gcatggtccg ccgcgaacgc gaactcggcg tacgcaccgc
     1141 catgggcgcc agccgtattc gactcttccg ccagctgctg actgaaagct cgctcctcgc
     1201 gctgatcggc ggcgcgctcg gcctttttct cgctgacagc ggtttgcagc tcctctccgc
     1261 ctacgtcgcc cgcttcaccc cgcgcgcccg cgaagtccac atcgatctga ccgttctcct
     1321 tttcaccctc ggcgtcgcgc ttttcgtcag cgtgctcacc ggcaccgccc ccgccgttgc
     1381 tcgccgcgaa aacctcatca ccgcgttaaa agaaggcggc aaccagtcca cgattggctt
     1441 cgtccgcgga agacttcgca gcactctcat cgtggcgcaa gtcgccgtct ctttcctcct
     1501 gctcatcggc gccggcctca ccgttcgcag cctcatcaat ctgcaacgcg tcgaccccgg
     1561 cttccgcccg gaaaatgtcc tcaccatcca attcagcctc gacttttcgc gctacacctc
     1621 caacgaaaaa actctcgcct tctggaattc actcctgcaa aaagctcggt ctttgccggg
     1681 tgtaatgtct gtagctgccg ccggcgattt ccccctcgac aaatcccccg ccttcagcaa
     1741 tgaatttctt atcgagggcc gcaccatcaa tcccaccgac gcaaaaccca ctgccgaact
     1801 caacgtaatc actccggaat atctcagcgt cctcggcatc ccgctctaca gtggccgcaa
     1861 tttcgatgcg cacgatcaac ccactagtcc tcacgtcgcg ctcatcaatc aatccaccgc
     1921 gcagcgtttc tggcacaacg aagatcccgt cggcaagcgc atcttgttcc agaatatcgg
     1981 cggcggcaca aactggacgc aaatcgtcgg cgtaatcggc gacgtccacg aacacggctt
     2041 ggacgttccc gtcaacgatt tcatctatct tcccttcgcc caatatccgc agaccggccc
     2101 cgcgctgatg gctcgcaccc agggcgaccc catggtcgta gcacgcgccg tagtccagcg
     2161 cctctacgag gtggacccca accagcccgc cggccgcatt caatcgctgg agcaataccg
     2221 cgccgactcc atcgccgcgc cgcgcctcac agccaacctt ctcggcctct tcgctctgct
     2281 cgccctggcc atcgctgccg ccggtattgg tggagtcatg gcactcgccg tcagccagcg
     2341 cacccacgaa atcggcgttc gcatggctat cggcgcccgc cccgcggaaa ttgtccgcat
     2401 gattctcagc cagggtatgg gcctcgccct cttgggagtc gtcattggcg tagccaccgc
     2461 cttcgccctc acccgcgccg ttaaatcgct cctgttcggt gtgactccga acgacccagc
     2521 cacctttctg ggcgtagccg ccgtcctcgc caccgccgct cttgccgcct gctacctacc
     2581 agcgcgtcgc gctgccaggg tcgatccctt gcgcgccttg cgcaccgaat agcgccgcgc
     2641 agccttacag gaacaccgta gggccatcta catggaccct cttatcgaca tccagcgcgc
     2701 gcctgataac gtaacctcac tcgctgccgc gggtcttact caccgttgcc gcgacgagat
     2761 tacggggtgt attcacccag cggcttattg ctcctgctca ccgggcgttt gaagcgcacc
     2821 ttcgcgtttc ccgcgctcgc cccctccacc aagccctggt tagtcccacc accattccac
     2881 gactgaggag cttcgcaatg aacgtcaacg agcgctctgc aagggtctgt gtgtctcctg
     2941 ccgttcctcg ccttaccgcc ctatccatcg ctgcgctgct ctttgcagtc acggccggtg
     3001 cccaatcccc cgcaaacccc cgcaacacct ccgtcgcgct aaccaaattc gtcgccgcca
     3061 ccaaccatct ttctcccgaa gtccgccgcc acctctcccg cggcatgcaa caatatcttc
     3121 gctacgccgg ttctgtagcc aacgccgtcg cctccccagc aacg
//
LOCUS       DASTPM010000032         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_404841, whole genome shotgun sequence.
ACCESSION   DASTPM010000032 DASTPM010000000
VERSION     DASTPM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404841"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFR42_00495"
     CDS             <1..545
                     /locus_tag="VFR42_00495"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEU0367657.1"
                     /translation="HCPSRRTGRFSAVAYPVEKQGNIVEHTTLSEDKTTQVVDTEARL
                     KNLTAYRDSLRAMLGKPGLNIKDSVEIQEKLTDVQSDLDGETAKRKILANETEKVTVE
                     IEFRVRDASSRRSSFAPLWSALGEAGETLAESLGSVVTFVVFIAPWILLFVVIWWFLR
                     RLWHKRRARKTAFAAAQTGS"
     gene            555..1091
                     /locus_tag="VFR42_00500"
     CDS             555..1091
                     /locus_tag="VFR42_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0367658.1"
                     /translation="MASKSYFLKSQRLGFRCWSRDDLPLARDLWGDLEVTKFFGGPFP
                     DGEIRRRLQVELDRAAAHNFQYWPMHLLDGGEFVGCCGLRPYKLEQGIPELGFHLCPK
                     FWGRGLAPEAAATVIHHAFHSLEFEVLSAGHHPDNASSKKVLEKLGFQYTHDEFFPAL
                     GMHIPYYLLTKPQEDGRN"
     gene            complement(1260..1496)
                     /locus_tag="VFR42_00505"
     CDS             complement(1260..1496)
                     /locus_tag="VFR42_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367659.1"
                     /translation="MFGSDIVDVALGMVFVYLLLSLICSAVKELIEQVMKHRARDLET
                     SLRELLADPDGTGLVQKIYNHGMIFGLFRISTTT"
     gene            complement(1527..2921)
                     /locus_tag="VFR42_00510"
     CDS             complement(1527..2921)
                     /locus_tag="VFR42_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367660.1"
                     /translation="MVKRLLLLLVLATAPLYGQENQLQADFRGEGERFQKGCLSFDLK
                     ALGSCAQVLFTDHPLHIAVGSIAPQNGFAAGAAFVAHWTPDETWRLNWNADAVASPNG
                     SWRAGVYMKAIRVPHHDITVTTGSAADAAASNLAVEEAPFFNVYAQAISLNKIYYFGL
                     GPSSTVANRSVFGMSERIVGTNVVWPIFGKLNLSLYGEVNGRFVDIRGNHSESSPSIE
                     TLFSEATAPGLTNQPGFAQFGEGARMRPHLLSDYLRLNYFVNFQQYVAGDSTFSFRRL
                     TVDLGHQIPLYRKSTRTLLPKNHNGPDACAVGTGADAQCPPVVRNLEGSINFRFFLAE
                     SFTSAGSVVPFYFQPTLGGGDLNGNTTLPSFQDYRFRAPNVMFLHEGFEHSIWKLPLG
                     VAFSADQGKVALNRGDLGSNPWLHTYSAGLTLRAGGFPQIFLLYSWGGGEGTHLIANV
                     NTSLLGGAARPSLF"
     gene            complement(2914..3207)
                     /locus_tag="VFR42_00515"
     CDS             complement(2914..3207)
                     /locus_tag="VFR42_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367661.1"
                     /translation="MAGKPKNIVRVPKPPRSAFDKERPASRLLLDQMIHVANALSMYL
                     RTFDNRAAQEPVTEEQAGHFVRVATSILHPHTAASSAKKKSGTARKKKRKGRG"
     gene            complement(3216..4433)
                     /locus_tag="VFR42_00520"
     CDS             complement(3216..4433)
                     /locus_tag="VFR42_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0367662.1"
                     /translation="MTAGLDNLRHIVVLMMENRSFDHMLGGLKKQDSRINGLTGNESN
                     LDTTNEPAQVQPKAQFQGQLDPDPDHHFPGVHQQLYFGTTGPPAAPSMNGFIQSYFQQ
                     QKDADHARKIMYYFPPEKLPVLSTLARSFAVFNGWFSSIPGPTICNRAFAHYGTSFGK
                     VGMDLFYENVPFKNIYDRMWGAGHTAKIYYFDQTSSTMEIVNLLKNQPQNFATYPQFL
                     DDCKKGLLPEYSFIEPNYNDHEGEGGEALASDQHPDHNIQQGEIFIASIYNAIRGNAV
                     LWKSTAMLLVYDEHGGIYDHVAPPNCKPDGFVASAADTGIGQEFKFDRLGVRVPAVLI
                     SPWIPKATVVAGPEDTNNGRTFEHASIPATVTKQFIGNYDARSPREKTAETFLDLLSD
                     NLRPDSDCPVFHF"
BASE COUNT         1048 a         1356 c         1294 g         1051 t
ORIGIN      
        1 gccattgtcc ttcgcgtcgt accggacgat tttccgcagt tgcttaccca gtcgaaaagc
       61 agggaaacat cgtcgagcac acaacactaa gcgaagacaa aacgacgcaa gtcgtggata
      121 ccgaggcacg gctgaaaaac ctgactgcgt atcgcgacag cctgcgagct atgctcggca
      181 agccgggctt gaacatcaaa gactccgtcg aaatacagga aaagctgacc gacgtccagt
      241 cggacctcga cggcgaaact gctaaacgaa aaatccttgc gaacgaaact gaaaaagtca
      301 ccgtcgaaat tgagttcagg gtgcgagacg cgagtagcag acgtagctcg ttcgcgcctt
      361 tgtggtcggc gctaggagaa gccggggaaa ccttggccga aagcctcggt tccgtagtaa
      421 ccttcgttgt gttcatcgct ccgtggatcc tgctgttcgt cgtgatttgg tggtttctgc
      481 gacggctctg gcacaagcgg cgcgcaagaa agacagcctt cgcggctgcg cagacgggct
      541 cctagttatt tagaatggct agcaaatcct actttctgaa atcgcagcgg ttgggatttc
      601 gctgctggtc gcgggatgat ctgccgctgg cacgcgacct gtggggcgat ctggaagtaa
      661 caaagttttt cggcgggccg tttcccgacg gagagatccg acgcaggctg caggtcgaac
      721 tagaccgagc ggccgcgcac aacttccaat attggccgat gcacctgctc gacggcggcg
      781 aattcgtcgg ctgctgtgga ctccgtccgt acaaactgga acaaggaatc cccgaattgg
      841 gcttccatct atgcccgaaa ttttggggac gtgggctagc gccggaagcg gccgccaccg
      901 taattcacca cgcattccac tctctcgaat tcgaagtact ctctgccggt catcatcccg
      961 acaacgcaag ctcaaaaaaa gtgctcgaga agctgggctt ccagtacacg cacgacgagt
     1021 tcttccccgc gttggggatg catattccct actatttgtt gacgaaaccg caagaggatg
     1081 ggcggaacta aaaacggcag gcatttctgg gttcttcggg ccacggttcg ggcggcagat
     1141 tcttcggctg gtagttcttg tcgcgttgca gcttgtcctt cacggtcgcg tgtagcaatg
     1201 aaccgtcggg aagagctcgc cgcgcaccga caggaactct cccgttcaac ttttgattat
     1261 tacgttgtag tacttatgcg gaagaggccg aaaatcatcc cgtgattata gattttctgc
     1321 accaggccgg tgccgtccgg gtcagccagc aattcgcgca ggctggtttc cagatctctc
     1381 gcgcggtgct tcatcacttg ctcgatcagt tccttcacgg cagaacagat caggctgagc
     1441 agcaagtaaa caaagaccat tcccagcgcg acatccacaa tgtcagaacc aaacataaaa
     1501 tctccgcgca aaacttcgta cgattttcaa aacagcgacg gtcgcgctgc cccgccgagc
     1561 agcgaagtgt tcacatttgc gatcaggtgc gttccttcgc cgcctcccca ggaatacaaa
     1621 agaaaaattt gcgggaagcc gcccgcacgc aaggtcagac cggcggaata cgtgtgcagc
     1681 cacggattgg aacccaagtc gccgcggttc aacgcgactt ttccctgatc ggcggaaaac
     1741 gccacgccca gcggcagctt ccagatggaa tgctcgaatc cttcatgtag aaacatcacg
     1801 ttcggcgcgc gaaagcggta gtcctgaaaa ctcggcagag ttgtattgcc gttcagatcg
     1861 ccgccaccga gcgtgggctg aaaataaaat ggcaccacgc tgcccgctga agtgaacgat
     1921 tcggcgagaa agaatcggaa gttaatgctg ccttccagat tgcgcaccac cggcgggcat
     1981 tgcgcatcag ctcccgttcc cacagcgcaa gcgtcagggc cgttgtgatt ttttggcagc
     2041 agcgtgcgcg tgcttttgcg atacagcgga atctggtggc ccaggtccac cgtcaggcgg
     2101 cggaacgaaa acgtcgaatc tcccgccaca tattgctgaa agttgacgaa gtaatttaac
     2161 cgcagatagt ccgacagaag atgcggccgc atccgcgcgc cttctccgaa ctgcgcaaat
     2221 ccaggctgat tcgtcagacc aggagccgtc gcctcggaaa aaagcgtttc gatcgacggg
     2281 ctggattcgc tgtgattgcc tcgaatatcc acaaagcggc cgtttacttc accatagagc
     2341 gaaagattca gctttccgaa gatcggccag accacattcg tgccgacgat tcgctcgctc
     2401 atgccgaaca ccgaacggtt tgctaccgtc gacgaagggc ccagaccgaa ataataaatt
     2461 ttgttcaacg agatggcctg cgcgtacaca ttgaaaaatg gagcctcttc gacagcgaga
     2521 ttggaggccg ccgcgtccgc cgcgcttccc gtggtcacgg taatgtcgtg atgcggcacg
     2581 cgaatcgcct tcatgtacac gcccgctcgc cacgatccgt tcggcgaggc taccgcgtcc
     2641 gcgttccaat tgagccgcca tgtttcgtcc ggcgtccagt gcgccacgaa cgcggctccc
     2701 gcggcgaatc cattttgcgg tgcgatgctg cccactgcga tgtgcagcgg atgatccgtg
     2761 aagagaactt gcgcgcaact tccgagcgct ttcaggtcaa atgacaggca gcctttctga
     2821 aaacgctcgc cttcgccgcg gaaatcagcc tgcagctggt tttcttgccc gtacaacggc
     2881 gccgtcgcta acaccaggag cagcagtaaa cgcttaacca cggcctttcc tctttttctt
     2941 tcttgcggtt ccgctcttct ttttggctga gctggccgca gtgtgcggat gcaagatgga
     3001 ggtggccacc cttacgaaat gtccagcctg ctcttccgta acgggttcct gtgccgcgcg
     3061 attgtcgaaa gttctcagat acatcgacag cgcattcgcg acatgaatca tctggtcgag
     3121 cagcaatcgc gatgccggcc gttccttgtc aaacgcagaa cgcggaggtt tggggacgcg
     3181 aactatattt ttcggtttgc ccgccattgg tcacctcaga aatgaaacac gggacagtcc
     3241 gaatcgggcc gcagattgtc cgatagcaga tccagaaacg tttccgccgt tttttcgcgc
     3301 ggcgaacgcg cgtcgtaatt cccgatgaat tgtttcgtca ccgtcgcagg gatggacgcg
     3361 tgttcgaacg tgcgcccatt gttcgtatcc tccggcccag caaccaccgt cgccttcggg
     3421 atccacggcg aaatcagcac cgctggaacc cgcacgccca atcgatcgaa cttgaactct
     3481 tgcccgatgc cggtatccgc ggcggaagcc acaaatccgt ccggcttgca gttcggcggc
     3541 gccacgtggt cgtagattcc gccgtgctcg tcgtacacca gcagcatcgc ggtgctcttc
     3601 cacagcacag cgttcccgcg aattgcgtta taaatcgacg cgataaaaat ttcgccctgc
     3661 tggatgttgt gatccggatg ctggtccgac gccagcgctt cgccgccttc gccttcatgg
     3721 tcgttgtagt tcggctcaat aaacgaatac tccggcagca gacctttctt gcagtcatcc
     3781 agaaactgcg gatacgtggc gaaattctgc ggctgatttt tcagcaggtt gacgatttcc
     3841 atcgtcgaac tggtctgatc gaagtaataa atttttgccg tgtgccccgc gccccacata
     3901 cgatcgtata tgttcttgaa cggaacattt tcatagaaca ggtccatccc gaccttccca
     3961 aacgacgtac catagtgcgc aaacgcgcgg ttgcaaatcg ttgggccggg aatggacgaa
     4021 aaccagccgt taaagaccgc gaagcttctg gcaagcgtcg agagcacggg caacttctcc
     4081 ggcgggaagt agtacatgat cttccgcgcg tggtcggcgt ccttctgctg ctgaaaatag
     4141 ctttggatga agccattcat cgagggcgca gccggtggcc cggtcgtccc gaaatagagc
     4201 tgctggtgca cgccggggaa atgatggtcc ggatcaggat ccagctggcc ctggaactgc
     4261 gcctttggct gcacctgcgc cggttcattc gtcgtatcga gattcgattc gttccctgtc
     4321 agcccgttga ttcgcgaatc ttgcttcttc agcccgccca gcatgtggtc aaacgagcgg
     4381 ttttccatca tgagaacaac aatgtgccta aggttatcca ggcctgctgt catgcgtgta
     4441 gtccccaact tcgaccggct ataagcgatg aactataagt agtgtaggga gacgccgcag
     4501 cgtttcaagt cacgattacg ttagatttca tgcctgccga aatgatgaat tttccgtggc
     4561 ggaaatcgct tgtttttggt taatgcaccg tctgcgattg atgcgcgcgc agattttcgg
     4621 tggtcgcgct cagctttttc aacatggtct ggatttccgg gtttgaattg aaaagtttcc
     4681 acaccaggcc ggttcgctga ttttcaatca ttacggtaat gggcgcctgg ttcagcccca
     4741 tataaatcg
//
LOCUS       DASTPM010000033         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_87715, whole genome shotgun sequence.
ACCESSION   DASTPM010000033 DASTPM010000000
VERSION     DASTPM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87715"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VFR42_00525"
     CDS             <1..956
                     /locus_tag="VFR42_00525"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEU0367663.1"
                     /translation="FVVSIVSSCPAQTSPTSEPYSVVHGWPQLADGFVLGQVSGVAVD
                     SHNHVFVFHRAENSWATDKTHVIASPTVLCFDGASGKLLSSWGQNRFLEPHGLRIDRE
                     DNVWLTDRALQQAFKFSHDGKLLLTIGTERTPGLDGTHFNLPADLAFAADGSVYVADG
                     YGNNRIAKFSPDGKFLLDWGRKGSADGEFDLPHSVAVDAQGLVYVADRNNARIQVFDA
                     NGKFLKTWKSNELGRPWSVTIGPDNLLYVVDGGDLKPNPPDRGQLLKLDLAGKIIAKW
                     SRFGNYDGQIYWGHDLAVGKDGAVYVGDVYHGMRVQKFIPH"
     gene            1011..2270
                     /locus_tag="VFR42_00530"
     CDS             1011..2270
                     /locus_tag="VFR42_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEU0367664.1"
                     /translation="MTSSSNSSKAAFYFILVTVGLDMIAFGIIAPVLPDLIRQFEGGD
                     FGRASAMIGYFGFAWATMQFIFSPILGAWSDRFGRRPVILLSCLGIGLDYIFMALAPS
                     LRWLFVGRLISGITTSNISTAFAYITDVTPAAERARKFGLLGAAFGLGFVIGPAVGGM
                     LGVHHLRMPFWAAAALSLANFFYGLFVLPESLPPERRAKSAWRMANPLGSLTLLRSHP
                     ELSALSIGITLYYLAHNSLPAMWAIYTEYRYAWNRRDVGLSLAAVGVCSALVSALLVG
                     PAVKRFGERVCLVAGLVFGTLGFLGFAFASRGTFAFAAIPLIALWGIAGPAIQSLMSQ
                     RVNPSSQGKLQGAINSLRSITGMAGPPLFTQVFTFAIAAKSALHFPGAPYFLAAVLLF
                     SSLLIAIAVLRAHTTSGSRAPSVPEPS"
     gene            2389..3036
                     /locus_tag="VFR42_00535"
     CDS             2389..3036
                     /locus_tag="VFR42_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU0367665.1"
                     /translation="MPKDFKSNHHAGTERSRPILHQHAREIQPYGKIAKLPIALDEST
                     CAESAENLNQVLADTITLRDLYKKHHWQVAGHTFYQIHLLFDKHYEEQNELVDTIAER
                     IQSLGAISLAMAPDVAETTIIPRPPRGREELPVQLSRLLEAHEFILKEARTMAKEASE
                     AGDDGTNDVLVSDVIRTNELQVWFLSEHLVDVPLVKADTPITEKEPAKSKSASVR"
BASE COUNT          565 a         1005 c          804 g          724 t
ORIGIN      
        1 tctttgtagt cagcattgtc tcgtcttgcc ctgcgcaaac ttcaccgacc agcgaaccat
       61 actccgtggt gcacggctgg ccacaactcg cggatggatt cgtgcttggc caggtttctg
      121 gagttgccgt ggactcgcac aaccatgtgt tcgtgtttca ccgcgctgaa aactcctggg
      181 ccacggataa aactcacgtc atcgcctctc caaccgtact ttgcttcgat ggtgcttccg
      241 gcaaactgct ctcctcctgg ggacagaacc gttttctcga accgcacggc ctgcgtatcg
      301 accgcgaaga caacgtctgg cttaccgatc gcgcgctgca gcaggccttc aagttttcgc
      361 atgacggcaa gctgctcctc accattggga cagagcgcac gcccgggctc gacggcacac
      421 actttaatct cccggcggac ctcgcgttcg ctgccgacgg aagcgtctac gtcgccgacg
      481 gctatggcaa caaccgcatt gccaagtttt ctcctgatgg caagttcctg ctggattggg
      541 gacgcaaagg ctccgccgac ggtgaattcg atctgccaca cagcgtcgcc gtcgacgcgc
      601 aaggcttggt ctacgtcgcc gaccgcaaca acgcgcgcat ccaggtcttc gatgccaacg
      661 gcaaattcct gaagacttgg aaatccaacg agcttggccg gccctggtcg gtcacaatcg
      721 gtcccgacaa tttgctctac gttgtcgatg gcggcgacct gaaacccaat ccacccgatc
      781 gcggccaact acttaagctc gacctcgccg gaaaaattat tgccaagtgg agccgcttcg
      841 gcaactacga cgggcaaatt tattggggcc atgatttggc cgttggcaaa gacggcgccg
      901 tgtacgttgg cgacgtgtat cacggcatgc gcgtacagaa atttattccg cactaagcga
      961 ttcgctggcg acaccgcgct ggctcggcta caatcttgcc ggagcagcgc gtgacttcgt
     1021 cttcaaactc ttccaaagct gccttttact tcatcctcgt aaccgtcggt ttggacatga
     1081 tcgcttttgg gatcattgcc ccggttcttc ccgatctgat tcgccagttt gaaggcggcg
     1141 actttggccg cgcctcagcc atgatcggct atttcggatt tgcctgggcc accatgcagt
     1201 tcattttctc gccgattctg ggagcctggt ccgaccgctt cggccgccgg cctgtcattc
     1261 ttctctcttg tctcggaatc ggactggatt acatttttat ggcgctggct ccgtctctcc
     1321 gatggttgtt tgttggccgc ctgatctccg gcatcaccac ttccaatatc tcaaccgcct
     1381 ttgcttatat caccgacgtc actccggcag cggaacgtgc caggaaattc ggattgctcg
     1441 gcgccgcctt cggactggga tttgtaatcg gtccggcggt tggcggcatg ctcggcgtcc
     1501 accatcttcg tatgccgttt tgggccgcgg cggctctgag tctggcgaat ttcttctacg
     1561 gactcttcgt cctgccggaa tcgcttccgc cggagcgccg cgccaaatct gcatggcgta
     1621 tggccaaccc gcttggctcg ctgacgcttc ttcgttcgca tccggagttg tccgctcttt
     1681 cgataggcat caccctctac tatcttgctc acaattccct tcctgccatg tgggcgattt
     1741 acaccgaata tcgctacgca tggaaccgcc gcgatgtcgg gctctcactc gccgccgtag
     1801 gagtctgttc ggcgctggtt tccgctttgc tggtcggtcc ggccgtgaaa cgcttcggag
     1861 aacgagtttg cttggtcgcc ggcctggttt tcggaaccct gggtttcttg gggtttgcat
     1921 ttgcatcccg cggcacattt gcctttgcag cgattccgct tatcgcactt tggggcatcg
     1981 ccgggccggc gattcaatcg ctgatgtctc agcgcgtcaa tccctcatcg caagggaagc
     2041 ttcagggcgc catcaactcc ttgcgctcca tcaccggcat ggccgggcct ccccttttta
     2101 cccaggtttt tacgttcgcc atcgctgcaa aatctgcact ccactttccg ggtgcaccct
     2161 atttcctcgc cgctgtgctg ttgttctcct cgctattgat cgcgatcgcc gttttgcgcg
     2221 cacacacaac ttccgggtct cgcgctccct ccgtccccga gccgtcctga gcggctcttc
     2281 gcccgtgggc taataccgca ttgacggagc gcgaattgtt tttctatcct cttcttcgcc
     2341 caccgcatcg aagggatatc gcacgcgccg atcctccaag gagctccaat gccaaaagat
     2401 ttcaaaagca atcatcacgc cggaacggag cgttcccggc cgattcttca ccagcacgca
     2461 cgcgagattc agccgtacgg caagatcgcc aagctgccca tagcgctcga tgaatccacc
     2521 tgtgcagaaa gcgccgagaa cctgaatcag gttctcgccg acacgattac tctccgcgat
     2581 ctctacaaaa agcatcattg gcaggtcgcc ggccacacct tctaccagat tcatcttctg
     2641 ttcgataagc actatgaaga gcagaacgaa ttggtggaca ccattgccga gcgcatccag
     2701 agcttgggcg cgattagcct ggccatggct cccgacgtcg ccgaaaccac catcatcccc
     2761 cgcccgccgc gcggccgcga agagcttccc gtccaactct cacggctgct ggaggctcac
     2821 gaattcatct tgaaggaagc ccgcaccatg gccaaggagg cctccgaagc cggcgacgac
     2881 ggcaccaatg acgtgctggt cagcgacgtg atccgcacca acgaacttca ggtatggttc
     2941 ctctcggaac acctcgtgga tgtgccgctc gtcaaggccg atacgccaat caccgagaaa
     3001 gagccggcca agtccaagtc ggcttccgtc cgctagtcat cacaactctt atcgttagtc
     3061 ttccaggggc tgccgcggca gatcagcggc ggcccctt
//
LOCUS       DASTPM010000034         5087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1269837, whole genome shotgun sequence.
ACCESSION   DASTPM010000034 DASTPM010000000
VERSION     DASTPM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269837"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFR42_00540"
     CDS             complement(<1..634)
                     /locus_tag="VFR42_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0367666.1"
                     /translation="MRKWCAAAAVGTLLALPLFAQQKDDTAKPATTAAAAPETTAAPE
                     SVAPLTRSLWAIPAIPRTTPFPGPQAATTTKDTRPPGKLLPRFEINGGYSYINFDPGD
                     PFRNFNNHGGSGGFTYNANRVLGLTAEVGGYNGHRDVNGVRQSAGLTSYLFGPRLNLR
                     MFDHFVPFAEFLFGGANGNLGFTGDQSQNAFALAAGGGVDVVLTKNLAWRF"
     gene            931..1866
                     /locus_tag="VFR42_00545"
     CDS             931..1866
                     /locus_tag="VFR42_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0367667.1"
                     /translation="MNPSAPFNIVDLDWTGRPQSIASLLIESQGFTALIDPGPESTLE
                     TLRNRLRQRGLQLSSLDAVLLTHIHLDHAGATGSLVRENPGLKVYVHEFGATHMADPS
                     RLLASAGRLYGDQLKTLYGDCLPVPETNLLWLAGSEKIQLGEVELDVFYTPGHASHHV
                     TYWNAASRIAFVGDTAGIRIEGKSYLLPATPPPDIDLERWNASLDTIASWNPERLFLT
                     HFGYIDNPAEHIRLYRERLSDWATLTQRLLDSSDSLESAERRFIEAIASEVRETLPAE
                     PAELYIFNGGLGLSWRGLVRYLKKKSVQTKPQASA"
     gene            complement(1914..3038)
                     /locus_tag="VFR42_00550"
     CDS             complement(1914..3038)
                     /locus_tag="VFR42_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU0367668.1"
                     /translation="MAVKIVRQPKKIALIGACSSAAAFLAGTEKAPAALRAAGLVEKL
                     RAAGFEVNDLGDCPPRMFADDEEHRRARNLLAIVAGLNDLKLHAETAIKSGALVLVLG
                     GDCAQVIGLLAGARRYYKHINLLWMDRDADLNTPASTPSGRIDGMVVAHAIGRGAPEL
                     VRFYGESALVREPDVLLFGLERVDPPEQEFLSKSPMRHVWAADIQKKGAAASMRDALA
                     HLHTESRDFVLHLDTDIIAQEEFPAVNMPGSGGLRLEEVRAALREALSQKTLLGLDVA
                     QYNPDRDADRSAAQKLVALLVEGLSVRFEAGSASEAPAAAPKAAVAVEPPSAPVVPAE
                     DAVAVEPVAGETVEEDAAAPETNVADSPAENSKSQSENSN"
     gene            complement(3146..4111)
                     /locus_tag="VFR42_00555"
     CDS             complement(3146..4111)
                     /locus_tag="VFR42_00555"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU0367669.1"
                     /translation="MVTDSNGRVLLRVRPRGFCAGVVRAVDIVELALEAYGAPVYVHH
                     EIVHNTYVVEQLRRRGAIFVESIDEVPHGAVLVFSAHGVPPGVRDEAKARSLKVIDAT
                     CPLVTKVHFEALKFAREERTIILIGHKDHQEIIGTSGEAPDRTIVVDSVEAVDKLEVD
                     NPEKLAFLTQTTLSLYDTQEIVSRLRQKFPKIVGPKSDDICYATQNRQEAVEQVAQDV
                     DLILVVGSQNSSNSNRLVEVAQRRGVKAELIDSASDIDPAWLQGVQRVGLTAGASAPE
                     VLVEQVSQRLAEHGYTDQRDLDLIREDVRFTLPPELASIAPAPRR"
     gene            4318..4986
                     /locus_tag="VFR42_00560"
     CDS             4318..4986
                     /locus_tag="VFR42_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEU0367670.1"
                     /translation="MKSRWLALTFLAVLGGLGLAGCDDHVVIDRDTTIPVRKGMTWAW
                     RPASPPAASNRPVTSRDTFHRRPPNYGPNSGANYDKVPAYERNSYWENDIVRNRIARA
                     FEQALNSRGLVQVSDPADADFLVDYQFAVQPRRERVATPLYSSGLVCGYYGCWNGFYG
                     PPGVYIHTVQYREGTIVFDLVDHRIKRLAYRAIRENVVHRDSFTDDEVREGVKHLLKG
                     LKPD"
BASE COUNT          984 a         1784 c         1240 g         1079 t
ORIGIN      
        1 cgaaccgcca cgccaggttc ttggtcagca ctacgtccac accaccgccc gccgccagcg
       61 caaaggcatt ctggctctgg tcgcctgtga atcccaagtt accgttggcg ccgccaaaca
      121 ggaattccgc gaacggcacg aagtggtcaa acatccgcag gttcagtcga ggaccaaaca
      181 aatacgacgt caaacccgcg ctctgccgaa ccccgtttac gtcccggtgg ccgttgtagc
      241 cgcccacttc tgccgttaac cccagcacgc ggttcgcgtt gtaggtaaag ccgccgcttc
      301 ccccatgatt gttgaaatta cgaaagggat ctcctggatc aaaatttata tagctgtatc
      361 cgccattaat ttcaaagcgc ggcagtaatt ttccaggcgg ccgtgtgtcc ttagtagttg
      421 tcgccgcctg cggtcccgga aagggagtcg tccgcggaat ggccggaatc gcccacaggc
      481 ttcgggtcaa cggcgctacg ctttccggag ccgccgtggt ttccggagcc gcagcggctg
      541 tcgtcgccgg cttcgctgta tcgtctttct gctgcgcgaa aagaggaagc gccaataacg
      601 ttcctactgc tgccgctgca caccacttac gcatatccat cctccctacc attcgttgta
      661 gaacttccgc ttgggtccca cgccccccgg ccaacagctg cgatcagagg gagcggcgca
      721 gcttgttacc ctgtctcttt tcatgcgcac aaattgaatc gacgcgtcct cacccttccc
      781 tgggttgttt atgggggagg aatagtaata agagacaaag cgcggtgccc atagcgtatg
      841 tcaacccgct ttctttccgc aagcaattcc ttgtcttttc atcagtttcc ggaaggcgaa
      901 tccgttcctc attgctacac tggtattggt atgaacccct ccgcgccctt taacatcgtc
      961 gatctcgact ggaccggccg tccccaatcc atcgcttctt tgctcatcga atcgcagggc
     1021 ttcacggctc tcatcgatcc cgggcccgag tcgaccctgg aaacacttcg caaccgcctc
     1081 cggcagcgcg gattgcaact ctcctcgctc gacgccgtgc tcctcacaca cattcacctc
     1141 gatcacgccg gcgccaccgg ctctctggta cgcgaaaatc ctggcctcaa ggtttacgtc
     1201 catgaattcg gcgccactca catggccgac ccttcgcgcc tgctggccag tgcgggccgc
     1261 ctttacggcg accagctcaa aactctttac ggcgattgcc ttcccgtgcc cgaaaccaat
     1321 ctgctctggc tcgccggcag cgaaaaaatc caactaggcg aagtcgagct cgatgtcttc
     1381 tacacgcccg gccacgcttc tcatcacgtt acctactgga acgccgcctc ccgcatcgcc
     1441 ttcgtcggcg acactgccgg catccgcatc gaaggcaaat cctatctctt gccagctact
     1501 ccgccacccg acatagacct ggaacgctgg aacgcttctc tcgacacgat tgcctcgtgg
     1561 aatccggagc gccttttcct cacccacttc ggctacatcg acaatcccgc cgagcacatc
     1621 cgtctctatc gggagcgcct cagtgactgg gcgacgctta cgcaacgtct gttggattca
     1681 agcgactcgc tcgaatccgc cgaacgccgc ttcatcgaag ccatcgcgag cgaggttcgc
     1741 gaaactttac ccgccgaacc cgccgaactc tacattttca atggaggcct gggactttcc
     1801 tggcgcggcc tggttcgtta tctcaagaaa aaatctgtgc agacgaaacc acaagcttcc
     1861 gcgtagcaat gtatcattgc gtcagaatta cggcgacgtt atcgcggcaa cgctcagttt
     1921 gaattttcgc tttgggactt tgaattttcg gccggggaat cggcgacgtt agtttccggc
     1981 gcagctgcat cttcctccac cgtctcgcca gcgacgggct ctaccgctac agcatcttcc
     2041 gcgggcacaa caggagcact gggaggctcg actgcgaccg ccgctttcgg cgcagccgcc
     2101 ggcgcctcgg atgccgagcc ggcctcgaaa cgtacgctca gcccctccac cagcagcgcc
     2161 accagctttt gcgcagcgga tctatccgca tcgcggtccg ggttgtactg cgccacatcc
     2221 agccccagca gcgtcttctg gctcaacgct tcgcgcaacg ccgcccgcac ttcttccagc
     2281 cgcaaaccgc cgcttcccgg catgttcacc gcagggaatt cttcctgcgc gatgatgtcc
     2341 gtatccagat gcagcacaaa gtcgcgcgac tccgtgtgca gatgggccag cgcgtctcgc
     2401 atgctcgccg ccgcgccttt tttctgaatg tccgcagccc acacatgccg catgggcgat
     2461 ttcgacaaaa attcctgttc cggcggatcc acccgctcca ggccaaaaag caaaacatct
     2521 ggctcgcgca ccagcgccga ttccccgtaa aacctgacca gctccggcgc gccgcgtcca
     2581 atcgcgtgcg ctaccaccat tccgtcaatt cgcccggaag gtgtggacgc cggggtattc
     2641 aaatccgcat cgcggtccat ccacaataaa ttgatgtgtt tgtagtagcg ccgcgcgccc
     2701 gccaacaatc cgattacttg ggcgcaatct ccgcccagca ccagcaccag agcgccgctc
     2761 ttgatcgccg tctcggcatg caacttcaaa tcattcagcc cagcgacgat ggccagaagg
     2821 tttcgcgcac ggcgatgttc ttcatcgtcg gcaaacatcc gcggcgggca atcgcccaaa
     2881 tcgttcactt caaatccggc agctcgcagt ttctcgacga gtcccgcagc gcgcaacgcc
     2941 gccggcgcct tctcggttcc tgccaggaac gctgcagcac tcgagcacgc acctatcaag
     3001 gcaatcttct tcggctgacg gacaattttg accgccaagg ctgtctcctg aagtttctga
     3061 aaaccgtgcc tgcgcttgcc tgacccgatc tacgttctac ttcccctgaa tcgacgggga
     3121 atcccccgcc cggtgaaact cgctcttatc gacgcggagc cggcgcgatg ctcgccagtt
     3181 ccggcggcag cgtaaaccgt acgtcttcgc gaatcaaatc caaatcccgc tggtccgtgt
     3241 agccgtgctc ggcaagccgc tggctcacct gctccaccag cacttccggc gctgacgcgc
     3301 ccgccgtcaa tcccacgcgc tgcactccct gcagccacgc cggatcgatg tccgaagctg
     3361 aatcaatcaa ctccgccttc acaccgcgcc gctgcgccac ttccaccagc cggttcgaat
     3421 tcgagctgtt ctgcgaaccc accaccagaa tcaaatccac gtcctgggcc acctgctcca
     3481 ccgcttcttg ccggttttgc gtggcataac aaatatcgtc gctcttcggc ccaacaatct
     3541 tcggaaattt ctgccgcagc cgcgacacaa tttcctgcgt gtcgtataag ctcagcgtcg
     3601 tctgcgtcag aaacgccagc ttttccggat tgtccacttc cagcttgtcc accgcttcca
     3661 ccgaatccac cacaatcgtc cggtccggcg cttcgcccga agtcccgata atctcttggt
     3721 ggtccttgtg cccgatcagg atgatcgtcc gctcctcacg cgcaaatttc agcgcctcaa
     3781 aatgcacctt ggtcaccaat ggacaagtcg cgtcaatcac tttcagcgaa cgtgccttgg
     3841 cctcatctcg cactcccggc ggcaccccgt gcgcgctgaa caccagtacc gccccgtgtg
     3901 gtacctcatc gatcgactcc acaaaaatcg ccccgcgccg ccgcaattgc tccacaacat
     3961 acgtgttatg aacgatttcg tggtgcacat acactggcgc cccataggct tccagtgcca
     4021 gttccacgat gtccaccgcg cgaaccactc ccgcgcaaaa ccctctcggt ctgactctca
     4081 gcaatactct tccgttggag tccgtcacca tctcactctc gtaccgaagt aatgtcttct
     4141 aatgtgctga tgccaaaact ctatcataac ccaccccccg gactgcctgc accgcttttc
     4201 cttttgttgt tcactggcct tcacttcacc agctcaccgg tcactaaagc gcaactttct
     4261 tgatcccagc gggttagatc tgaacgagcg cctcctctac ggcgcgaggg aacagccgtg
     4321 aaatcacgtt ggttagcgct caccttttta gcagtcctgg gaggcctggg cctcgccggt
     4381 tgcgacgatc atgtcgtgat cgaccgcgac accaccatcc ccgttcgcaa aggcatgacc
     4441 tgggcctggc gccccgccag tccgccggcg gcttcgaacc gaccggtaac ttcgcgcgac
     4501 accttccatc gccgtccacc taactatggc ccaaatagcg gcgccaacta tgacaaagtc
     4561 ccggcctacg aacgcaactc gtattgggag aacgacatcg tccgcaaccg catcgcccgc
     4621 gctttcgaac aagctctgaa ttcccgcggc ttggttcagg tcagcgaccc cgccgatgct
     4681 gacttcctgg tcgactacca gttcgccgtg cagcctcgac gcgagcgcgt tgccaccccc
     4741 ctgtatagct ccggcctcgt ctgcggctac tacggctgct ggaacggctt ttatggccct
     4801 ccgggcgtgt acattcacac cgtccaatat cgcgagggca ccatcgtctt cgacctggtc
     4861 gaccatcgca tcaagcggct cgcctatcgc gccatacgcg aaaacgtcgt ccaccgcgac
     4921 tccttcaccg acgacgaagt ccgcgagggc gtaaagcatt tgctcaaggg tctgaaaccg
     4981 gactgagcgc gacgccggct ctttccaaac gtgcctcatg gaatcggccc cgagttggcg
     5041 gggccgtttt tgttttcctt tcgcgaccgc ttgcgctagt cttgttt
//
LOCUS       DASTPM010000035         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_981605, whole genome shotgun sequence.
ACCESSION   DASTPM010000035 DASTPM010000000
VERSION     DASTPM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981605"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFR42_00565"
     CDS             complement(<1..1193)
                     /locus_tag="VFR42_00565"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0367671.1"
                     /translation="MPTLDSLNGKTFSHYRILERLGGGGMGVVYKAEDTRLHRFVALK
                     FLPENVAKDAQALARFEREAQAASALNHPNICTIHDIGEQDRQAFIAMELLEGSTLKH
                     RIGARPIEVETLLDLAIQIADALDAAHSKGIVHRDIKPANLFVTERGHAKILDFGLAK
                     VKSPVGVASGADSLATVGVDTDQLTSPGSTLGTAAYMSPEQVRGKELDARTDLFSFGV
                     VLYEMGTGTLPFRGETSGVITDAILNKAFVSPLRLNPDLPPKFEDIVNKALEKDRDLR
                     YQSAAEMRADLKRLRRDTDSGRISSSATSASAYAETVRRDASVPGSALNSAVRESASS
                     AGMAVRQAASGSRTRYIAAAALAVVLIAGAVYGAYHFGLGSRSSSTLAKITQISHWDK
                     AMFSPR"
     gene            complement(1287..>2023)
                     /locus_tag="VFR42_00570"
     CDS             complement(1287..>2023)
                     /locus_tag="VFR42_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0367672.1"
                     /translation="YHDQWLSEGFAEFAAGLFLQQAVGPKWQKDYLDFWERLRTRILE
                     KNNFGVAPNDSGPLWLGLRLVSPKTAQGYQNVTYPKGAYVLNMLRSLMYADQSTSGNP
                     DQALIDMMHDFTESHQKSPASTESFKQIAEKHMTKRMDLQQNGRLDWFFNEWVYGTAV
                     PKYSFKYDLESEPGDKTKLQVEITQSEVDRNFAMFVPVFADFGKGMMRLGQVAVVGNS
                     TRTAIFHMDNKPKKVALNAFKDVLER"
BASE COUNT          390 a          635 c          544 g          454 t
ORIGIN      
        1 cgcggcgaaa acattgcttt gtcccagtgg ctgatctgag tgatcttggc cagcgtgctg
       61 gaactgcggg agcccagtcc aaagtgatag gcaccataca ctgcgcccgc gatcaaaacc
      121 acggccaacg ctgctgccgc aatgtatctg gtgcggctgc cggatgccgc ttgcctcact
      181 gccatacccg cactgctggc cgattctcga acggcggaat tcaaggcgct gcccggcacg
      241 ctggcgtcgc gacggacggt ctcggcgtag gctgacgcag acgttgccga cgaggaaatc
      301 cgccccgaat ccgtatcccg ccgcaatctc ttcaaatcgg cccgcatctc cgccgcggac
      361 tggtagcgca gatcgcggtc tttctccagc gccttgttga cgatgtcctc gaattttggc
      421 ggcaaatccg gattcagtcg caaagggctg acaaacgctt tgttcaatat cgcgtcggtg
      481 attactcccg acgtttcacc gcggaatggc agcgtgcccg tccccatctc atacaacacc
      541 acgccaaacg aaaaaaggtc cgtgcgtgca tccaactctt ttccgcgcac ttgctcgggc
      601 gacatatacg ccgctgtccc cagcgtgctt cccgggctcg tcagttggtc tgtatcgacg
      661 ccgaccgtgg cgagcgaatc cgcgccactg gcgaccccaa caggcgattt caccttcgcc
      721 aagccgaaat ccagaatttt ggcgtgccca cgctcagtca cgaagaggtt ggccggcttg
      781 atgtcgcgat gcacaatgcc cttcgaatgc gcggcgtcga gcgcgtcggc aatctggatc
      841 gccaggtcca gcagcgtttc cacttctatc gggcgggcgc cgatgcggtg cttcagcgtc
      901 gagccttcga gcagttccat ggcgatgaac gcctgcctgt cctgctcgcc gatgtcatga
      961 atggtgcaga tgttgggatg attcaaggcg gaggccgctt gcgcttctcg ttcgaagcgc
     1021 gccaatgcct gcgcgtcttt ggccacattt tcaggaagga atttcagggc cacgaagcgg
     1081 tgcagccgcg tgtcctcggc cttgtacacc acacccatac ccccaccgcc gagccgttcg
     1141 aggatgcggt agtgcgaaaa agtcttgccg ttcaaagaat ctagggtggg catgtgcgca
     1201 gcatcttagg gcgcgctaat gtcgcaagtc aacgaactcg cgctcttgtc cgcatcgcta
     1261 atctctttcc gccgccgcta gacatttcac cgttccagga catccttaaa tgcgttcagt
     1321 gcgacctttt tcggcttgtt gtccatatga aaaatcgcgg tccgcgttga gttcccaacc
     1381 acggccactt gtcccagccg catcatgcct ttgccaaaat ccgcgaacac cggcacaaac
     1441 atagcgaagt tccgatccac ttcgctctgc gtaatctcca cctgcagctt ggtcttgtcg
     1501 ccaggttcgg attccaggtc gtacttgaag gaatacttcg gcaccgcggt accgtacacc
     1561 cattcgttga aaaaccagtc caaccgtccg ttctgctgca ggtccatgcg tttcgtcatg
     1621 tgcttttcgg ctatttgctt gaatgattct gtggacgccg gcgatttctg gtggctttcc
     1681 gtaaagtcgt gcatcatgtc aatcaaagct tggtccgggt tcccgctggt gctctggtct
     1741 gcgtacatca gcgagcggag catgttcagc acgtaggcgc ccttcggata ggtcacgttc
     1801 tgatagccct gcgcggtctt cggcgaaacc agcctcaagc ccagccacag cggacctgaa
     1861 tcgtttggcg caactccgaa attattcttt tccaggattc gagtccgcag gcgttcccaa
     1921 aagtctaagt agtccttctg ccactttggt ccgaccgctt gctgcaggaa caacccggcc
     1981 gcaaactccg cgaaaccttc ggacagccac tggtcgtgat acg
//
LOCUS       DASTPM010000036         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1039319, whole genome shotgun sequence.
ACCESSION   DASTPM010000036 DASTPM010000000
VERSION     DASTPM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039319"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..825)
                     /gene="phoU"
                     /locus_tag="VFR42_00575"
     CDS             complement(22..825)
                     /gene="phoU"
                     /locus_tag="VFR42_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU0367673.1"
                     /translation="MGSSAGLTETVLYDTLVNYLISMARTVEGAVNRALEAIVSVDDP
                     KAAALTGEVFLLEPRINEMEIVIDEHAIRLLRRGGFSDDEMRLIVASLKITNDLERIG
                     DLAVNLAERVTSLREMPGSHAPEELEAMTQAVKAMMTKSLGALIFRNATMATRVLESD
                     DLVDQFRDRIFEQLLDQMTKQTALVVPSMQFILATRHLERIADHATNIAEDIIYWVRG
                     LDVRHGRGLGISPVPASGEEEINETCDEVTQNSHGLYSDVNLASDTLSV"
     gene            complement(927..>2439)
                     /locus_tag="VFR42_00580"
     CDS             complement(927..>2439)
                     /locus_tag="VFR42_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0367674.1"
                     /translation="ENHAGRPEIAQAMAEGSGRSIRHSATVNRELLYYAFRQPSAVGK
                     SVVLRFAEPEETVGEVLGPFRKSLGLASLAILLVASGLILLVSRNFSERIEKLTAFSQ
                     RVAEGDFRPQASDGTGDALDRLSHSLNQTAARLDRTIHTLTEERNLSSAILGSMVEGV
                     LVVNGAERVVFANQSFAEILGLKVPPQPGSGLVEVVRQTELIEAARKVLAGEPRVEAE
                     IVTGTLRQHFFAATVASVRAAETNGAVIVLHDITDLRKLERVRRDFVANVSHEFRTPL
                     TAIQGFAETLLAGAMNDPQNRERFLGIIVEHSRRLARLTEDLLMLSKMDADRLELETR
                     RIPVGPFVESCIETSTPRAKDKDLKLLVNLAERVPDIAGDRRRLTEVLQNLLDNAIQY
                     TPAGGQIMVSAGPKNGEVIFTVSDTGIGIPQADQPRIFERFYRVDVARSREVGGTGLG
                     LSIAKHLIENHGGKIWVESEVGRGSQFHFSVPIFDLERAVPRGPASGGRAPAN"
BASE COUNT          483 a          806 c          620 g          530 t
ORIGIN      
        1 ggtaggttcg atcactgaag ttcaaacgct gagagtatcc gaagccaggt tcacatccga
       61 ataaagccca tgtgaatttt gcgtgacctc gtcgcacgtc tcgttgatct cttcctcgcc
      121 gctcgccggt accggagaaa ttcccaagcc gcggccgtgc cgcacgtcca acccgcgcac
      181 ccagtaaata atgtcctctg cgatgttggt ggcgtggtcg gcaatccgtt ccaggtggcg
      241 cgtggccaga ataaactgca tgctcggcac caccagtgcc gtttgcttcg tcatttgatc
      301 cagcagttgc tcaaaaatcc tgtcgcggaa ctgatccacc agatcgtcgc tttccagcac
      361 ccgcgtggcc atggtggcat tccggaaaat caacgccccc aggcttttcg tcatcatggc
      421 ctttacggcc tgcgtcatcg cttccagctc ctccggagca tgcgagcccg gcatctctcg
      481 caagctggtc acccgctctg ccaggttcac cgccaagtcg ccaattcgct ccagatcatt
      541 ggtgattttc agcgacgcca cgatcaaccg catctcatcg tcgctgaaac ccccgcgccg
      601 cagcagccgt atggcgtgct catcaatcac aatttccatt tcgttgatgc gtggctcgag
      661 caggaaaact tctccggtca aggccgccgc tttgggatca tccacactga caatcgcttc
      721 caatgcgcgg ttcacggccc cttccacggt ccgcgccatc gaaatcaggt agttcaccag
      781 cgtgtcgtac aacacggttt cggtaagtcc ggcggacgag cccatggagc cctctttccg
      841 agcgagaatc tttctccacc cgtacttcga gaatacgcaa acgtcattac agcaaagtga
      901 ttggctgatg aaacttcggt gagaattcag tttgcaggcg ctcgcccgcc gctcgccgga
      961 ccgcgcggca cggcacgctc tagatcgaaa atagggacgg aaaagtggaa ctgcgaaccg
     1021 cgcccgactt cgctctccac ccagattttg ccgccatggt tttcaatcag gtgtttggca
     1081 atcgataacc ccaggccggt acctccgact tcgcgcgacc tcgccacatc cactcgataa
     1141 aaccgctcaa aaattcgcgg ctggtccgct tgaggaattc ctattcccgt gtcggacacg
     1201 gtaaaaataa cttcaccatt cttcggcccg gcgctgacca tgatttgccc gcctgccgga
     1261 gtgtactgaa tcgcgttgtc taacagattt tgcagaactt ccgtaagccg ccttcggtcg
     1321 cccgcaatgt cgggaactcg ctcggccaaa ttcaccagta atttcaagtc tttgtctttt
     1381 gcgcgcggcg tcgaagtctc gatgcagctt tccacgaacg gtcctactgg gatgcgccgc
     1441 gtttccagtt ccagccggtc ggcatccatt ttcgaaagca tcagcaggtc ttcggtcagc
     1501 cgcgccagtc tgcgcgaatg ttccacgatg attcccaaaa agcgctcgcg gttttgcgga
     1561 tcgttcatcg ctcccgccag caacgtctcc gcgaacccct gaattgccgt cagcggtgtg
     1621 cgaaactcgt gcgatacgtt ggccacaaag tcgcgccgca cccgttccag tttccgcaaa
     1681 tccgtaatgt cgtgcagcac gatcactgcg ccattggttt ccgcggcgcg caccgacgcc
     1741 accgtggccg cgaaaaagtg ctgtcgcaag gttcccgtga caatttccgc ttccacgcgc
     1801 ggttcgcccg ctagcacctt gcgggccgcc tcgatcaatt cggtctgccg taccacctcc
     1861 accagcccgc tgcccggctg tggcggcact ttcaatccca gaatttccgc gaagctctgg
     1921 ttcgcgaaca ccacgcgctc ggctccgttt accaccagca cgccttccac catgctcccc
     1981 agaatcgccg acgaaagatt tcgctcttcg gtaagcgtat gaatcgtgcg gtccaggcgc
     2041 gccgcggtct ggttcagcga atggctcagc cgatccagcg cgtcgccggt gccgtctgaa
     2101 gcctgcgggc gaaaatcacc ctccgccact cgctgcgaaa atgcggtcaa cttttcgata
     2161 cgttctgaaa agttcctcga aaccaggagg atcaagccgc tggcaaccag caagatcgcc
     2221 agcgatgcca accccaggct cttgcgaaaa ggccctagca cttccccgac agtctcttct
     2281 ggctccgcga aacgcagcac aacggatttg cccacggctg acggttgccg gaacgcgtaa
     2341 tacaatagtt cgcggttgac cgttgcactg tgccggatgg agcggccgct gccttccgcc
     2401 atcgcctggg caatttctgg ccgtcctgcg tgattttcc
//
LOCUS       DASTPM010000037         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_462741, whole genome shotgun sequence.
ACCESSION   DASTPM010000037 DASTPM010000000
VERSION     DASTPM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462741"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VFR42_00585"
     CDS             <1..839
                     /locus_tag="VFR42_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367675.1"
                     /translation="TLEAIADGKRRVIPTSVAPITFFVREDAEWMIPRREDAEALPSG
                     LNADASAVLRFLRQRGASFFADIVRGTGKLKAEVETALWELVAAGLLTADGFDNLRAL
                     IDPKRRAGQGSGKAARPRHSTGRWSLLYSGLYSGEEQDRGRALEATCWMLLKRYGIVF
                     RELLTRESMVAKWRELLMTFRRLEDRGEIRGGRFVSGFLGEQFALPVAVESLRARRKQ
                     SPVGEITTVSAADPLNLVGIVVPGERVAANSGRVVAFRDGVAAGTDDDWPGRSHVVAM
                     AR"
     gene            complement(1242..2522)
                     /locus_tag="VFR42_00590"
     CDS             complement(1242..2522)
                     /locus_tag="VFR42_00590"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEU0367676.1"
                     /translation="MKKLLVLLLALSWPGLTRADAVLDWNETAASAAFAAGLDNLTDG
                     CVDALHESRIFAMMHAAIHDALNAIERRSQPYALDAQAPAGSSPEAAVAAAAHDVLVA
                     VFPQLPSNLGLTPAAATDLVETAYITALAAIPDTPAKAQGILIGQAAAAAILALREAD
                     GSDALFLDFAYVPGPNPGDFQLIPGLPFAAAPAWGDVTPFVLTSSSQHRPKDPYELSS
                     KKYAADFNEVKSLGAVNSSTRTAEQTEIALFWIEGAPQGWNRIARIVSAQYGLDLWEN
                     ARLFGLLNLASADGYIADFENKYFFKFWRPITAIRAADTDGNPDTVADPMWDSLRGTP
                     PAPDYTSGHSVEGGAMSEVLTRFFGTQVSFSTSSTMQPGVTRSFTSFSQAAEENGNSR
                     IYVGFHFRHAVTEGIKLGNKVGEVAFNHYLQPVH"
     gene            complement(2544..>2735)
                     /locus_tag="VFR42_00595"
     CDS             complement(2544..>2735)
                     /locus_tag="VFR42_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367677.1"
                     /translation="GNRWCIARLTCAAEIESALSMNPAQLIVAPFFAERRKRVVRHSL
                     ARPLGKNPRGLLSDQQTSE"
BASE COUNT          549 a          730 c          903 g          553 t
ORIGIN      
        1 cgacacttga agccattgcc gacggaaagc gccgggtgat cccgacgagt gttgcgccga
       61 ttactttttt tgtgcgcgaa gacgcagagt ggatgattcc ccggcgcgaa gatgccgagg
      121 cgcttccctc cggactgaat gcggacgcgt cggcggtttt gcgctttttg cggcagcgtg
      181 gcgcttcatt ttttgcggat atcgtgcgcg gcacggggaa actgaaagca gaagtcgaga
      241 ctgcgctgtg ggaactggtc gccgctggat tgctgacggc ggacggcttc gacaatttgc
      301 gtgcgctgat tgatccaaag aggcgtgccg gccagggcag cgggaaggcg gcgcggccac
      361 ggcacagcac cggccggtgg tccttgcttt actcggggct ttactcgggt gaagaacaag
      421 accgcggacg cgcgctggaa gcgacatgct ggatgctgtt gaaacgatac ggaattgttt
      481 ttcgcgagct attgacacgg gagtcgatgg tggccaagtg gcgcgaattg ctgatgacgt
      541 tccggaggct ggaggatcgc ggagaaattc gcggcgggcg atttgtgagc ggctttctgg
      601 gcgaacagtt tgcgttgcct gtagcggtgg aatcgctgcg ggcgcggcgg aagcagagtc
      661 cggtgggaga aattacgaca gtgtcggcgg cagacccgct gaacctggtt ggaatcgttg
      721 tgccgggcga acgagtggcg gcaaattccg ggagagtggt ggcgtttcgg gatggagttg
      781 cagccggcac ggatgatgac tggcctgggc gatcacatgt ggtggcgatg gcgcgctaaa
      841 agcctaaaga gacttggcgc gtccagcagt caacaattcg ttgtatttgt agctgccggg
      901 aattttgtgc tatcacttgc ccccgcgcca gcgcggccca gcttgcctgc gatcactgaa
      961 ttgtgattcg agggccagat gccggcgaaa aatccagaac cgaaaaacga gcaccggcga
     1021 aacagcaacg caatcgcaga tgcagcgaag agggtagccg gagcaattat gatcatgaag
     1081 cgcagcggtc ggcgcgaacg tgagatttgc tgcggtgtcg agaactgtgg cgctgcgagc
     1141 cgcgccagat gcagcgagag ataatcttaa ttgcacacaa gcgtctcgaa tccattggtc
     1201 tgatcgtcag gcccgccgcg tgagactcgt ttctttccgg gttaatgaac cggctgcagg
     1261 tagtggttaa aggcaacttc gcccaccttg ttccccagtt taattccctc ggtgacggcg
     1321 tgccggaaat gaaagccgac gtagatgcgc gagttgccgt tttcttccgc cgcttgggag
     1381 aagctcgtga atgaacgagt gacccccggc tgcatcgtgc tgctggtact gaagctgacc
     1441 tgggtgccga agaaccgcgt cagcacctct gacatcgctc ctccctcgac gctgtgtcct
     1501 gaggtgtaat ccggagcggg tggggtccca cgcaatgagt cccacatcgg gtcagcgaca
     1561 gtatccgggt tgccgtcggt atcggcggca cggattgcgg tgatcggtcg ccagaacttg
     1621 aagaagtact tgttttcaaa atccgcgatg tagccgtccg ccgaagcgag attgagcagg
     1681 ccaaacaggc gcgcattctc ccacagatca aggccatact gggccgacac aattctcgcg
     1741 atcctattcc acccctgggg agcgccttct atccagaaca gggcgatttc ggtttgttcg
     1801 gcggtgcggg tgctactgtt cactgcgccc agacttttta cctcattgaa atccgccgcg
     1861 tatttcttgc tgctcaattc gtaggggtcc ttcggccggt gctgcgagct ggaagtaagc
     1921 acgaagggag tgacatctcc ccatgctggt gcagcggcga acggaagccc ggggatgagc
     1981 tggaaatctc ccggattggg ccctgggaca taagcaaaat caaggaagag ggcgtccgac
     2041 ccatccgctt ctctcagggc gaggatggct gcggcggcag cctggccgat caggataccc
     2101 tgggccttgg ccggggtatc ggggatcgca gcgagcgccg tgatgtaggc cgtttcgacg
     2161 aggtcggtgg ctgcggctgg ggtcaagcca agattactag ggagttgagg aaagacggcg
     2221 acgagcacat catgtgcagc cgcggccacg gcagcttcgg gggaagaccc cgcgggcgcc
     2281 tgggcgtcca gggcgtaagg ctgggaccgc cgctcgatcg catttagcgc atcatggatt
     2341 gccgcgtgca tcatggcaaa gatccgcgat tcatggagcg cgtccacaca cccatccgtt
     2401 aaattgtcca ggccggcagc gaaggcggcg ctggcggcgg tctcgttcca atcaaggact
     2461 gcatcagccc tggtaagccc tggccaactc aaggcgagaa gtagaactag tagctttttc
     2521 atagggcctc cttttgttgt gtttcattcg ctggtctgtt gatcggagag aagccccctc
     2581 ggattcttgc ccaaaggacg agcgagggag tgcctcacca ctcttttgcg gcgctcggcg
     2641 aaaaacggcg ccacgatgag ctgggcagga ttcatcgaga gtgcactctc aatctccgca
     2701 gcacacgtaa ggcgcgcaat gcaccagcgg ttgcc
//
LOCUS       DASTPM010000038         6124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_289785, whole genome shotgun sequence.
ACCESSION   DASTPM010000038 DASTPM010000000
VERSION     DASTPM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289785"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1177
                     /locus_tag="VFR42_00600"
     CDS             59..1177
                     /locus_tag="VFR42_00600"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU0367678.1"
                     /translation="MKTSSAKKTIEAAIEEVVALARRVVPPKMTAAVLYGSEDLRIEE
                     IEVPSLAPDEVLLRVRLALTDGTDLKVWKRGYHAKMIRPPAVFGHEVVGEVVAVGKRV
                     DARWRMGMRVVAANSAPCFRCYHCRRGQENLCDDLLFNNGAYAEYMRIPGRIVTENML
                     EVPHSVDDQSAALVEPLACVLRGIHEMELRTGDTAVVIGCGPIGLKFVRMLSRRGIRV
                     IALARRDAPLDVARKLGAFATINVSTTPDPVATARALTEDQRGPDAVVEAAGNPSAWQ
                     LALGMIRRGGVVNFFSGLPSGTKVEIEPAAIHYSEIKVISTFHHTPRFIREALEAVRR
                     GDISAQDFVTEEIRLADLPQAFERMKARSGEIKLAVRP"
     gene            1255..3219
                     /gene="hpnC"
                     /locus_tag="VFR42_00605"
     CDS             1255..3219
                     /gene="hpnC"
                     /locus_tag="VFR42_00605"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEU0367679.1"
                     /translation="MLRPGELEIAKNAPPAGCSRESAQKYTRWLATHHYENFNVASWL
                     LPKELHQHFYNLYAYCRWADDLGDEIPDTRRALELLDWWEHELNLCYEGRPSHAVFVA
                     LRETIVAKDIPRQPFADLLKAFRQDQTVKRYHTWESVLDYCVYSANPVGRLVLYLCGY
                     RDEPRQRLSDATCTALQLANFWQDVSRDLEKGRIYIPLDAAARHGVTEDDIVSRRFSQ
                     NYVALMRNLIDRTRKLFNKGLPLARKVEGKLSIDLEMFSRGGIAVLDAIEAQGCDTLN
                     RRPSISKTKQASLLATVLVKQWFSRKPEVSTNGPAAARHAVSSSAVSRPVIIDETNVA
                     ASYEACHRIARAARSNFYYAFYLLPKAKRDGLAALYAFMRLVDNVADDGNDLAAKHRG
                     LAKWRAALDEAVTSYGAFVDDSSALALQAGPPGASRVLPALVDTMRRFDMPARYLHDQ
                     ISGAEMDLTVKSYPTFDRLREYCYRVAGTVGLTCTHIFGFEDSRALDLAEKLGLAFQL
                     TNIIRDVSEDAERGRVYLPDEDLARFNVSPADFSKNQATLGVRELLRFEADRAFQLYE
                     EGSELLDLIDADSRGTLWLLIHTYNALLARIESLDFAVCGERIRLSKAEKMLFIARAR
                     FARHTRDNVLEKRDRDWRRAGGAGGRRRAG"
     gene            3149..4591
                     /gene="hpnE"
                     /locus_tag="VFR42_00610"
     CDS             3149..4591
                     /gene="hpnE"
                     /locus_tag="VFR42_00610"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEU0367680.1"
                     /translation="MSSKNVIVIGGGLAGLAAGVALADAGCRVRLFEQRPYLGGRATS
                     YALPNGEHVDNCQHVTLGCCTNLEDFYKRVGSAGKIKFFDRLFFLDPQGRTGVMQAGF
                     LPAPAHMSGSFLAFDPLSLKDKLSIARALAAILFAGGQPKDAYPPAQPVSMLGWLRRH
                     KQTPRAIERFWRVVLVSALDEELDRTDARFGIDVFWKAFLKNSTGYRMGVPSVPLAEL
                     YDGCRIAIEQKRGEVILRSPVRNLCLKNGAIAAVQFDGGREESADALVLAVPHDRLSE
                     LLPAEVRAANPALAHLEKFKVSPITGVHFWFDRQVTSEPFITLLDTQTQWIFNKTALY
                     VSSVGAQHVAPQPATNAGQYLQLVISASYDLLKKSREEIIELCLKEVRQAFPAAYQAQ
                     LIKATVIKEVAATFSPEPGVDQWRPGQETRTPGLFLAGDWTATGWPATMEGAVRSGYL
                     AAEAVLRSVGAPQEFLQPDLPASGLCRLFD"
     gene            complement(4754..4975)
                     /locus_tag="VFR42_00615"
     CDS             complement(4754..4975)
                     /locus_tag="VFR42_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367681.1"
                     /translation="MELTKQGKHNAALELLEWSDCRIAAISGETADLHAISPCGSHRR
                     ATCGNLSAASYHYEPSLRANLKMHLPYTV"
     gene            5626..6108
                     /locus_tag="VFR42_00620"
     CDS             5626..6108
                     /locus_tag="VFR42_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367682.1"
                     /translation="MDRKYSHRGYRDSEKDDKKSRPEKKPPSGGPRGQNDHLGPRTPR
                     MVGTVTRARCANCGAVLAPGFDPSGTCPKCNTELHSCKQCRFFDSGAKFECMQPIPER
                     ITPKDGKNNCKFYEFRMTVEKDTAPTSYQQPAQAAAVAEAMPSRPLDARQAFENLFKK
                     "
BASE COUNT         1214 a         1934 c         1655 g         1321 t
ORIGIN      
        1 ggctggaact ggctgcgaac ccgactgcgg agtcgttgaa agtcttgatt acgcacgaat
       61 gaaaacatca tccgcaaaga agaccatcga agcggctatt gaagaagtcg tagctctggc
      121 gcggcgtgtc gttccgccga aaatgacggc ggccgtgctc tacggcagtg aagacttgcg
      181 tattgaagaa attgaggttc cctcgctggc ccccgatgaa gttttgctgc gtgtgcgcct
      241 tgcactgacc gacggcaccg acctcaaagt ctggaagcgt ggctaccacg ctaaaatgat
      301 tcgtccgccg gccgtgttcg gccacgaggt cgtcggtgaa gtagtcgccg tcggcaaacg
      361 ggtggacgca cgctggcgca tgggtatgcg cgtcgtggca gctaattccg ctccctgttt
      421 ccgctgctac cattgccgcc gcggtcagga aaatctttgc gacgatctgt tgttcaacaa
      481 tggcgcttac gccgaataca tgcgcatccc cgggcgtatc gtcacggaaa atatgctgga
      541 agtgccgcac tcggtggatg atcaaagcgc ggcgctggtg gagccgctgg cttgcgtgtt
      601 gcgcggcatt cacgaaatgg aattacgcac cggagacaca gctgtggtga tcggctgcgg
      661 ccctatcggg ctgaagtttg tacgcatgct ttcgcgccgc ggcattcgcg tgatcgcctt
      721 ggctcgccgc gatgcgccgc tcgatgtggc gcgtaagctc ggcgcttttg cgacgatcaa
      781 tgtttccaca acacctgatc ccgtagctac ggcgcgagcg cttactgaag atcagcgcgg
      841 cccggacgcc gtggttgaag ccgctggtaa cccatctgcc tggcaactcg ctctgggcat
      901 gattcgccgc ggcggcgtcg tcaacttctt tagcggcttg ccttccggca ccaaggtgga
      961 aattgagccg gccgccatcc actattccga aattaaagtc atttcaactt ttcatcatac
     1021 tccgcgtttt attcgcgaag cgctggaagc ggtgcgccgc ggcgacatct ccgcgcagga
     1081 ctttgtcacc gaagaaatcc gcctcgccga cctgccgcag gctttcgagc gcatgaaagc
     1141 gcgcagcggc gaaatcaagc tggccgtccg cccgtgataa tgctgtttgg ttcttgtcaa
     1201 aatctccgcg ctgtctgcct tcccggcgct ttctgtgttt aaacttttct attgatgctg
     1261 cgccccggtg aactcgagat cgccaagaac gccccgcccg cgggctgttc gcgggagtcc
     1321 gcgcagaaat acacgcgctg gctggccacg catcactatg aaaatttcaa tgtcgcgtcg
     1381 tggctgctgc ccaaggagct gcaccagcac ttctataatc tctacgccta ctgtcgctgg
     1441 gctgacgacc tcggcgacga aattcccgac acgcgacgcg ccctggaatt gctcgactgg
     1501 tgggaacacg aactgaatct ctgttacgaa ggccgccctt cacatgccgt gtttgtagcg
     1561 ctccgcgaaa ccatcgtggc gaaagatatt ccgcgccagc ctttcgccga tttgctcaag
     1621 gcctttcgcc aggaccagac ggtcaagcgc taccacacgt gggaatccgt tctcgattat
     1681 tgcgtttact ccgcgaatcc cgtcggccgg ctcgtgttgt acctttgcgg ctatcgcgat
     1741 gagcctcgcc agagactttc cgacgcgact tgcacggccc tgcagcttgc caacttttgg
     1801 caggacgttt cgcgtgactt ggaaaagggc cgcatctaca tcccgctgga cgcagccgcg
     1861 cggcatggcg tcaccgaaga cgacatcgtc tcgcggcgtt tcagccagaa ctacgtcgcg
     1921 ctgatgcgaa atctcatcga ccgcacacgc aagctcttca acaaaggact tccgctagcg
     1981 cgaaaggtcg aaggcaaact cagcatcgac ctggaaatgt tcagccgggg cggcatcgcc
     2041 gttctcgacg ccatcgaagc gcaaggctgc gacacgctga accgccgtcc gtcgatcagc
     2101 aagaccaaac aagcatcgct gctcgcgacg gtcttggtga agcagtggtt ctcccggaag
     2161 cccgaagtga gcactaacgg ccccgcagca gctcgtcatg cggtatcctc ttccgccgta
     2221 tctcgcccgg taatcattga cgaaacaaac gtcgctgctt cctacgaagc ctgccaccgg
     2281 atcgctcgcg cagcgcgcag taatttctac tacgcgtttt atcttctccc gaaagcaaaa
     2341 cgagacggct tggccgccct ctacgcgttc atgcgcctgg tggacaacgt cgccgacgat
     2401 ggcaacgacc tcgccgccaa gcatcgcggg ctcgccaaat ggcgcgccgc cctcgatgag
     2461 gctgtcacta gctacggcgc attcgtcgat gacagttcgg cgctcgccct ccaggcgggt
     2521 ccgccaggcg ccagccgagt ccttccggcg ctggtggaca ccatgcgccg gttcgacatg
     2581 cccgcgcgct atctgcatga tcagatctcc ggcgccgaaa tggacctgac cgtcaagtcg
     2641 tatccgacct tcgaccgtct tcgcgaatac tgctatcgcg tggccggcac cgtcggcctg
     2701 acatgcacgc acatcttcgg tttcgaggat tctcgcgcgc ttgatcttgc cgaaaaactc
     2761 ggcctcgctt ttcagctcac caacattatt cgcgacgtga gcgaagatgc cgagcgcggc
     2821 cgtgtttatc ttcccgatga agacctcgct cgcttcaacg tttcgcccgc tgacttttcc
     2881 aaaaatcaag ccacgctcgg cgtccgcgaa ctgctgcgct ttgaagccga ccgcgccttc
     2941 cagctttatg aagaaggctc agagcttctc gacctgattg acgccgacag ccgcggcact
     3001 ctctggctgc tcatccacac ctacaacgcg cttctcgcgc gcatcgaatc gctcgacttc
     3061 gctgtctgtg gcgaacgcat ccgcctcagt aaggcggaaa aaatgctctt catcgcccgc
     3121 gcacgcttcg cgcggcatac acgggataat gtcctcgaaa aacgtgatcg tgattggcgg
     3181 cgggctggcg gggctggcgg ccggcgtcgc gctggctgac gcaggctgcc gcgtacgact
     3241 ctttgagcag cgcccctacc tcggcggccg cgcgacttcc tacgcgctgc cgaatggcga
     3301 gcacgtggac aattgtcagc atgtcacact cggctgctgc accaacctcg aagattttta
     3361 caagcgcgtc ggctccgccg gcaaaatcaa atttttcgat cgcctgtttt ttctggaccc
     3421 acagggtcgc actggtgtga tgcaggcggg atttcttccg gcgcccgcgc acatgagcgg
     3481 ctcatttttg gccttcgatc cgctttcgtt gaaagacaag ctctccatcg cgagagccct
     3541 tgccgcgatt ctttttgccg gtggccagcc caaagacgct taccctcccg cgcaacctgt
     3601 ctcgatgctc ggctggttgc ggcgccacaa acagactccg cgcgccatcg agcgcttctg
     3661 gcgggtagtc ctggtcagcg cgcttgacga agaactcgac cgcaccgacg cccgttttgg
     3721 cattgacgtc ttctggaaag cttttctcaa gaacagcacc ggctatcgta tgggcgttcc
     3781 gtccgtcccg ctggccgagc tatatgacgg ctgccgcatt gctattgaac aaaaacgtgg
     3841 cgaagtgatt ctgcgttcgc cggttcgcaa cttgtgtcta aagaatggcg caatcgccgc
     3901 agtgcagttt gacgggggca gggaagagtc cgccgacgct ctcgtgctcg ccgttccgca
     3961 cgacagactg agcgaattgc tgcccgcgga agttcgcgcg gcgaatcccg cgcttgctca
     4021 tctcgaaaaa ttcaaagtct cacccattac cggcgtccat ttctggttcg accgacaggt
     4081 caccagcgag ccgttcatta cgctgctgga cacgcagacg cagtggattt tcaacaagac
     4141 cgctctgtat gtgtcaagtg taggggcgca gcatgttgcg cctcaaccgg caaccaatgc
     4201 aggccaatat ctgcagctgg tgatcagcgc gtcctacgac ctgctcaaaa aatcgcgcga
     4261 agaaatcatc gaattgtgct tgaaagaagt ccgccaggcc tttcccgccg cgtaccaagc
     4321 ccaactcatc aaagccacgg tcatcaaaga agtcgccgcc accttttcgc cggagcccgg
     4381 tgtagatcaa tggcgcccag gacaggaaac caggacgccc ggactattcc ttgccggcga
     4441 ttggaccgct accggctggc ccgccacgat ggagggcgcc gtccgcagcg gctacctcgc
     4501 cgccgaagct gtcctccgct ctgttggcgc gccgcaagaa ttcctccagc ccgaccttcc
     4561 cgcctcgggc ctctgccgcc tgtttgacta atggcgggtt tatcgttcct gagtggatca
     4621 ggccattcct ttttcaacca gctcccggaa gaccctgtct caaaatttgg tcgtcacctt
     4681 agaccaagcc ggcgtggcat tttcgcggca actttggcct tctcgggatc accctgttcg
     4741 agtgcggtag cggctagacc gtataaggca aatgcatttt caggtttgcg cgcagggacg
     4801 gttcgtagtg gtagctggcc gctgagagat tcccacaggt cgcgcggcga tgactgccgc
     4861 atggtgagat agcgtgcaag tcggctgttt ctcctgaaat agcagcaatc cggcaatcgc
     4921 tccattccag taactccagt gctgcgttgt gcttgccttg tttcgtcagc tccatgcttg
     4981 atggatcgcg cagcggaatc cgttttgttt gctcactgcg cagccttaca aattctacgg
     5041 ttggtcatgc ctctgcaata tgtctttcgc tcgcagtgca accttcgcat attcggtcga
     5101 tctccgcgta ctcctttgca tattttttcc gaaactcctc atacgcccca cgcgacttcc
     5161 acatatccat cgtcacgtac acgcgtgcac ggcttacgtc acgtagcagc cgcgtgccgc
     5221 aataacgcgc atcgcgccgg aacagctgta cccactcgcc ctccggccca tatgcctgct
     5281 caaacaattc ttcacttcct gacttgactt cgaattccca tagagttaca aacaaagatg
     5341 tcccatctcc tgcttctcgg atgttggcaa tccttttgaa cctgaatcct agtgcagtat
     5401 ccattgccgc gccgagtgta gcaatccgat tggatcaaat ttcagtatcg gtttacagaa
     5461 tctgagacaa gacaggattt cagggttgct ttacaaaccc tgggataacc agagaacagc
     5521 gtatcaatag ctttgaagct cccatttccc ggcctgaggg tgtaactctg cagtccagga
     5581 gccgcttccc caggctccgc cgggtttgct ttgcacggag gcgtcgtgga tcgtaagtac
     5641 agccatcgcg gttaccgcga ttccgaaaaa gacgacaaaa aatcacgtcc tgaaaagaag
     5701 ccaccctccg gtggcccgcg cggacagaac gatcatctcg gcccccgcac tccgcgcatg
     5761 gtcggcaccg tcacccgcgc ccgctgcgcc aactgcggtg cggttctcgc tcccggtttc
     5821 gatcccagcg gcacatgccc caagtgcaat accgagctgc acagctgcaa gcaatgtcgc
     5881 ttcttcgatt ccggggccaa attcgaatgc atgcagccga ttcccgaacg catcactccc
     5941 aaagatggca agaacaattg caagttctat gaatttcgca tgaccgtgga gaaggacacc
     6001 gctccaactt cctatcaaca gcccgcacag gccgccgctg ttgcggaagc catgccgtcg
     6061 cgcccgctcg acgctcgcca agccttcgaa aatctgttca agaaatagcg gagcgttttg
     6121 tcga
//
LOCUS       DASTPM010000039         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1183575, whole genome shotgun sequence.
ACCESSION   DASTPM010000039 DASTPM010000000
VERSION     DASTPM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183575"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFR42_00625"
     CDS             <1..414
                     /locus_tag="VFR42_00625"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367683.1"
                     /translation="LRYDLSPDHVRYVLQQLREVLTRHPKLDETSARVRLLKLGENAI
                     EAEIYGYILTREYREFLAVQEELILQAMDVLEASGAAVALPTQTTMVTRDAWIDPQKA
                     AAAQKAMEKTRDPGVPGMQRAEFAADVADIIPPKH"
     gene            complement(429..899)
                     /locus_tag="VFR42_00630"
     CDS             complement(429..899)
                     /locus_tag="VFR42_00630"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEU0367684.1"
                     /translation="MQKHRVVAIDTGIAEQVRGTLKSPVYGFPALLEDSGEPAPCRHC
                     LRSIAANEGRILFTYDRFSGKEQLPQPGPIYIHAKSCPRFSESDGFPEDLRNSPRTVE
                     AYGNGRNLKAQAYVTDGGFEPVIANLFADSEVDYLQIQSTTAGCFTFRVERAAK"
     gene            888..1070
                     /locus_tag="VFR42_00635"
     CDS             888..1070
                     /locus_tag="VFR42_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367685.1"
                     /translation="MLLHDVVSTVVILGPDAAAKAYCGCVVRSFLSAMSDQTIFVLAP
                     SAFPRFELILGIALAA"
     gene            1197..2558
                     /locus_tag="VFR42_00640"
     CDS             1197..2558
                     /locus_tag="VFR42_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0367686.1"
                     /translation="MLRNRLLITLGGCAVLASAAWADGVAYVDCTSHSEDTQVFGKPR
                     RSPDVVASLPCGERFTVIQNGFIFSRIQTKDGKIGYVYSNVISQDPGGKIGPRAAEQA
                     PAVAAKPPVTPALPPAATPAPVTPAAQPAATIAPASTSAPVQPTVQASSSQAQAAPEA
                     AKPAPAKPPVATSILPTPPTTLEAAAEKALEAAQQGNKTENAPATVAPAEVSPAAPAA
                     APVPSATSASTPAAASAANVPESSAAPAQPEAAPAASAQPEAAAAQPEPAPAPIRATP
                     RESWERPNPGGIRRTPLIELFGGYSFARVDNGSGSFSNLNGALGSFGWNAKPWLQVVA
                     DSSYNLVTIGGVKNVLYGNHWGARIFGRSRNRWGLTPFVEGLAGGSRADAKVSGAGGY
                     STSANCFSFKAGGGLDLRPSKHWELRLLNVDYYRTAFGTGLHQNNYTASVGIVLRLFG
                     GAE"
BASE COUNT          478 a          762 c          849 g          529 t
ORIGIN      
        1 ctgcgctatg acctttcacc ggatcatgtg cgctacgtgc tgcaacaact gcgcgaagtt
       61 ttgacgcgcc accccaagct ggacgaaacc agcgcgcggg tgcgtttgct gaagctgggg
      121 gaaaacgcca tcgaagcgga aatttatggc tacattctga cccgcgagta ccgcgaattc
      181 ttggcggtgc aggaagaatt gattttgcag gccatggatg tgctggaggc gtcgggcgcg
      241 gcggtggcgc tgccgacgca aaccaccatg gtcacgcgcg atgcctggat cgatccgcaa
      301 aaagccgccg cggcgcaaaa ggccatggag aaaacccgcg atcccggcgt ccccggcatg
      361 cagcgcgccg aattcgccgc cgatgtcgcg gatatcattc cgccaaaaca ctaagatccg
      421 acggattgct attttgccgc gcgctctacg cggaaggtga agcagccggc ggtggtgctt
      481 tgaatctgca ggtaatccac ttcgctatcg gcgaatagat tggcgatgac cggctcgaag
      541 ccgccgtcgg tcacgtacgc ttgcgctttg aggttgcggc cgttgccgta ggcttccacg
      601 gtgcgcgggc tgttgcgcag gtcctctggg aatccgtcgc tttcggaaaa acgcgggcag
      661 ctcttcgcgt gaatgtaaat cggccccggc tgtggcagct gttcctttcc gctgaagcga
      721 tcgtaggtga acaggattct tccttcattc gctgcgatgc tgcgcaggca gtggcggcaa
      781 ggggcgggtt cgcctgagtc ttccagcagg gcagggaagc cgtaaacggg agatttgagc
      841 gtcccgcgca cttgctccgc gattccagta tcgattgcga ctacccgatg cttctgcatg
      901 atgttgtctc cacggtggtt attttggggc cggatgccgc cgccaaggcc tattgtgggt
      961 gtgttgtccg ctcgtttcta tccgcgatga gcgatcaaac aatttttgtc ctggcgccgt
     1021 ccgccttccc cagatttgaa ctcattttgg gcatcgcttt agccgcgtag tggtaaccgt
     1081 cccttcgtcc agtactgttt tgggaattgc tggcctttgc gccctttgac cagcacgccg
     1141 caaggcttga taactgtaag caggggctcg gcttgggtgc cgagtggggg gtttgcatgt
     1201 tgcgtaatcg attgctgatc actttgggcg gatgtgcggt gctggcctcg gcagcttggg
     1261 ccgatggcgt cgcgtacgtg gattgcacca gccattccga agatacgcag gtgttcggca
     1321 agccgcgaag gtcgcccgac gtggtggctt cgctgccgtg cggcgagcgc ttcacggtaa
     1381 tacaaaacgg attcatattt tcgcgcattc aaaccaaaga cggaaagatc ggatacgtct
     1441 actcgaatgt gatttcgcag gaccccggcg gaaaaattgg gccaagggcg gcggaacagg
     1501 cgcctgcggt ggcggccaag cctcccgtta ctcccgctct tcctcctgcc gctacaccgg
     1561 ctccagtgac gcctgcggcg caacctgcgg cgacaatcgc gccagcttcg acgtcggcac
     1621 cagtacagcc aactgtgcaa gcttcctcat cgcaggcgca agcggccccg gaagcggcca
     1681 agccggcacc agcgaaaccg ccggtagcca catcgattct gccgactcct cccacgacgc
     1741 tggaagctgc ggcggagaag gctctcgaag ccgctcaaca gggcaacaag actgagaacg
     1801 cgccagctac cgtggctcct gcggaagtaa gtcctgcggc gcccgcggcg gcgcctgtgc
     1861 cgagcgcgac ttcggccagc actccagcgg ctgcgtcggc ggcgaacgtt ccggagagtt
     1921 ctgctgcgcc ggcacaacct gaggcggccc cggccgcgtc tgcgcagccg gaagctgctg
     1981 cggcacagcc tgagcccgct ccggcaccga ttcgggcgac cccacgcgaa agctgggaga
     2041 ggccgaaccc gggagggatt cgccggactc ctttgatcga actctttggc ggctattcct
     2101 ttgcgcgggt tgacaatggc agcggatcgt tcagcaacct gaacggtgcg ctgggatcgt
     2161 ttgggtggaa cgcgaagccc tggctgcagg tggtggcgga cagcagctac aacctggtaa
     2221 ccatcggcgg cgtgaagaac gtgctgtatg gaaatcattg gggagctcgc atttttggcc
     2281 ggtcacgcaa tcgctgggga ctgacgccct ttgtggaagg gttggccggg ggatcgcgcg
     2341 cggacgccaa ggtgagcggc gccggaggtt attcgacatc ggccaactgc ttttcgttca
     2401 aggcgggcgg cgggttggat ctgcggccgt cgaagcactg ggaactgcgg ctgctgaatg
     2461 tcgattacta ccggactgcg tttgggaccg gtctgcacca gaacaattac accgcgtcgg
     2521 tgggaattgt gctgcgactg tttggcggag cggagtaaga cttggccacc tgaggcattg
     2581 tgacggtcag gccttgtgcc cgataaagcc agtctttc
//
LOCUS       DASTPM010000040         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1327725, whole genome shotgun sequence.
ACCESSION   DASTPM010000040 DASTPM010000000
VERSION     DASTPM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327725"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1050
                     /locus_tag="VFR42_00645"
     CDS             211..1050
                     /locus_tag="VFR42_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEU0367687.1"
                     /translation="MDLFEEIVRMRKDGMRGALATIVHTNGSIPSYESSRMLVRDDGT
                     IVGTIGGGCVEADVWAAAKEVMEKESPRKMTFQLNNEASYDNGLICGGTLEVFVEPIL
                     PQPVAYIFGGGHISMALAKSASAAGFGIVVIDDREQFANKERFPMAQELYTSYEDAFA
                     RLTPNAASYIAIVTRGHRDDMRVLAWAVRTHAHYIGMIGSKRKVISVYQALEKEGYAP
                     EEFSHVYAPMGLEIGALSPEEIAISITAEIVAVRRNSSSAQHKKLQREAREPAVSARK
                     TSR"
     gene            1062..1649
                     /locus_tag="VFR42_00650"
     CDS             1062..1649
                     /locus_tag="VFR42_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU0367688.1"
                     /translation="MLAAVILSGGASRRMGSPKALVSYQGASFLEHLLSVTRHPAIGV
                     RRVVLGPDAEEISAQIALAPQEVVLNKDWEQGQLSSIQAALRTLPPGTEGMLLCPVDH
                     PLVSATLVNSLIVTFLETRAPVVLPMFEGRRGHPVIFAAAVYEELLRAPLDVGARAVV
                     WSHQHDVQEVSTTEEGCVLNLNDPGAILKSKNKRS"
     gene            complement(1675..2028)
                     /locus_tag="VFR42_00655"
     CDS             complement(1675..2028)
                     /locus_tag="VFR42_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU0367689.1"
                     /translation="MFSSQVLDHFQNPRNAGELRDATASVEVDNPVCGDVLRLAIKVE
                     QGRIAQARFRCRGCTTSIACASQLTVLLCGNSLDRLREISVGELAESLGGLPDETLHG
                     AHLAIDAARALATKV"
     gene            complement(2070..>2389)
                     /locus_tag="VFR42_00660"
     CDS             complement(2070..>2389)
                     /locus_tag="VFR42_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367690.1"
                     /translation="GSAVEVPMRVRLKLDLLKKKRIDWPRIENDNELMTAGIYRPVDD
                     AVRIAVSELVHWIHSDYGLSDLDAYELCSKVCKLHLTEMVDPNYVVVASMEKKYLPSR
                     IAK"
BASE COUNT          495 a          628 c          783 g          483 t
ORIGIN      
        1 acgaggactt cgccgaagcg cggctcgggc tcagggacct cggtgtagcg gaggacgtcg
       61 ggagtgccgt ggtggtcaaa gacgatggcc ttcatggtgc gaggaatcgt agcgtggaca
      121 cgccggagcc ggcaagatgc cggcggtacg acggaggagt tgcgttgaca ggatggggtg
      181 ggcggcatag gctcaatcag gaactcatcc atggacctgt ttgaagagat tgtgcggatg
      241 cgcaaggacg ggatgcgcgg ggcgctggct acgattgtgc ataccaatgg ctcgatcccg
      301 agctacgaga gctcgcggat gctggtgcgc gacgacggca cgattgtggg gaccatcggc
      361 ggcggctgcg tggaagccga cgtgtgggcg gcggcgaaag aagtgatgga gaaggaatcg
      421 ccgcgcaaga tgacgtttca gctgaataac gaagcgagct atgacaacgg gctgatttgc
      481 ggcggcacgc tggaagtttt tgtggagccg attctgccgc agcctgtggc gtacattttt
      541 ggcggcggac acatatcaat ggcgctggcg aaatcggcga gtgcggcggg attcgggatt
      601 gtggtgattg acgaccgcga gcagtttgcc aacaaagagc ggttcccgat ggcacaggag
      661 ctgtacacga gttatgagga tgcctttgcc cggctgacgc cgaatgccgc gtcgtacata
      721 gcgattgtga cgcgagggca tcgcgacgac atgcgggtgc tggcgtgggc ggtgcggact
      781 cacgcgcact atattggaat gatcggcagc aaacgcaaag tgatttcggt atatcaggct
      841 ctggaaaaag aaggctatgc gccggaagag ttctcgcatg tgtacgcgcc gatgggattg
      901 gagattggcg cgctttcgcc ggaagagatt gccatcagca tcacggcgga gattgtcgcc
      961 gtgcggcgca attcgagttc agcccagcac aagaagttgc agcgcgaagc tagggagccc
     1021 gctgtttcgg cacgtaagac ttctcgctga caggattcgt aatgctggct gcggtaatcc
     1081 tttctggcgg tgcttcgcgc cgcatgggtt cgccaaaggc tctggtgtcc taccagggcg
     1141 cgtcgtttct ggaacacttg ctgtccgtta cgcggcatcc ggcgatcggt gtgcggcgag
     1201 tcgtgctggg gccggatgcg gaagagattt ccgcacagat cgccttggct ccgcaagagg
     1261 ttgtcctcaa caaagactgg gagcaggggc aactaagctc gattcaagca gcattgcgga
     1321 ctttgccgcc tgggacagag ggcatgctgc tgtgcccggt cgatcatccg ctagtcagcg
     1381 caacgctggt caacagtttg attgtgacgt ttctcgagac acgcgcgccg gtggtgcttc
     1441 ctatgtttga gggccgccgc ggccacccag tgatttttgc ggctgcggtc tacgaggagt
     1501 tgttacgagc gccactcgac gtcggagcac gcgcggtggt ttggtcgcac caacacgacg
     1561 tgcaggaagt ttccacgacc gaagaaggct gcgtgctgaa cctgaatgat ccgggcgcga
     1621 ttctcaagtc aaaaaacaag cggtcttaat tttggcgtga agagtttttc accgctaaac
     1681 cttcgtagca agagcgcgcg cagcgtcgat ggccaagtgc gcgccgtgca acgtttcatc
     1741 cggtaagccg ccgagcgatt ctgccagctc gccgaccgaa atttcgcgca gccgatccaa
     1801 ggaattcccg caaagcaata ccgtcagttg cgacgcacaa gcgatagaag tagtgcatcc
     1861 tcgacagcgg aaacgcgcct gcgcgatcct tccctgctcc actttgatgg ccagccgcag
     1921 cacatcgccg caaaccggat tgtcgacttc caccgacgcc gtcgcgtccc gcagctcgcc
     1981 tgcgttgcgt ggattctgaa aatgatcgag gacctggctg gaaaacatgg cttgagtatg
     2041 accactttgc gacgaaccta gcaaccgcgc tatttggcga tccgcgaagg aagatacttc
     2101 ttttccatcg aagcgaccac gacgtagttc gggtcgacca tttccgtcag atggagcttg
     2161 cagactttgg agcacagttc gtaggcatcg aggtcagaca ggccatagtc ggagtggatc
     2221 caatggacaa gctcgctgac ggcgatgcgg acggcatcgt ccaccggacg gtagatgccg
     2281 gcggtcatca gttcgttgtc attttcgatt cgcggccagt cgatacgttt tttcttaagc
     2341 aggtcgagtt tcagccgaac gcgcatcgga acctcaacag cgctgccgg
//
LOCUS       DASTPM010000041         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_30483, whole genome shotgun sequence.
ACCESSION   DASTPM010000041 DASTPM010000000
VERSION     DASTPM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30483"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFR42_00665"
     CDS             <1..505
                     /locus_tag="VFR42_00665"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEU0367691.1"
                     /translation="VPNAYLPVLARAAFLHDIGKMGIPDKILLKPGPLTDDEKVIMRK
                     HCQIGYDMLIRIPFLRDAAEIVLAHQEFFDGTGYPRGLKGEEIPLGARIFAIADSMDA
                     MLSDRPYRKALPMSYAREEIQRCAGTQFDPKVVKVFLSIPELHWKELRETLGSPFRLA
                     HLKNLEA"
     gene            580..1719
                     /locus_tag="VFR42_00670"
     CDS             580..1719
                     /locus_tag="VFR42_00670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0367692.1"
                     /translation="MSSPKQSVQDIQPVPQMDLSAQYAAIGAEIRDAVTRVLASQHFV
                     LGKEGAALEQEIAQLCRVRHGIGVASGTDALILALRAVGVKAGDEVILPPFTFVATGS
                     AVSALGARPKFADIDPATFNISVAQIEKRITPRTRAIVVVHLYGLAADMDSIREVAAK
                     HRLPIVEDNAQAIGATYKGRSTGSLGDIACFSFYPTKNLGAYGDAGMITTDSPELDAR
                     LRTLRNHGQASRYNSVEPGWNSRLDEIQAAILRVKLRHLKEWERRRRAHAAQYARLLA
                     GIAEVQPPQVPSTREHVFHQYTIRAQRRDELQKFLTDRKIGNAVYYPVPLHLQPIYAN
                     EGFPAGSLPESEKAAAEVVSLPMFPELRLEQMERICSAITEFYGR"
     gene            complement(1739..2428)
                     /locus_tag="VFR42_00675"
     CDS             complement(1739..2428)
                     /locus_tag="VFR42_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU0367693.1"
                     /translation="MPGTVADDIELIIEEIHGDGGGKPPSRDGDDDGGGDDGGGGEHA
                     PEPRPPGSKKFSIAVLIGMVSIVMLFLVLTAAFVELRVQHLHTWIGIRIPKILWANTV
                     VLLLSSATLEMARRKLRLDDAAGFRWMWALTTALGILFVGGQVIAWWQLAQQGVYVTS
                     RLATSFFYVFTALHAVHLLGGVCALLYVALHKFDAARVSRSLAAQLASYYWHFMDGLW
                     VFLLALLYFGR"
     gene            complement(2572..2658)
                     /locus_tag="VFR42_00680"
     tRNA            complement(2572..2658)
                     /locus_tag="VFR42_00680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2622..2624),aa:Leu,seq:gag)
     gene            complement(2786..3268)
                     /gene="secG"
                     /locus_tag="VFR42_00685"
     CDS             complement(2786..3268)
                     /gene="secG"
                     /locus_tag="VFR42_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU0367694.1"
                     /translation="MPGWMKWGVALLIGFGLIIVGFRWAGWLLATFFVLNCLVLIIVV
                     LLQSGKAADLAGAFGGAGSQTAFGPRGAATVLSQATTWCAVMFMVCAMALALRSERSV
                     GGGSSVIYNSTKPAATKPAAPATTPAAPATQPAGTQSAPAPSQQTPAPASPAQSPKKQ
                     "
     gene            complement(3321..>3656)
                     /locus_tag="VFR42_00690"
     CDS             complement(3321..>3656)
                     /locus_tag="VFR42_00690"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU0367695.1"
                     /translation="EEVLSQQFIGGPGALTREEFSRILLAYEPVWAIGTGRTATPEIA
                     AAAHRFLRQCVRDQFSPEAAASVRILYGGSVKPDNIQGLMAQEELDGALVGGASLDAK
                     SFATIVNTK"
BASE COUNT          777 a         1246 c          959 g          674 t
ORIGIN      
        1 ggttcctaat gcctatttgc cggtgctggc gcgcgccgct ttcctgcacg acatcggcaa
       61 aatgggcatt ccggataaga ttttgctcaa gcccggcccg ctcacggacg acgaaaaagt
      121 catcatgcgc aagcactgcc agatcggcta cgacatgctg atccgcattc cttttttgcg
      181 cgacgccgcg gaaatcgttc tggcccacca ggaatttttt gacggcaccg gctatccccg
      241 cggactaaag ggcgaggaaa ttcctctggg cgcgcgcatt ttcgccatcg ccgacagcat
      301 ggacgccatg ctcagtgacc ggccgtatcg caaggcgctc cccatgtcct acgcacgaga
      361 agaaatccaa cgctgcgccg gcacacagtt cgacccgaaa gtcgtcaaag tgttcctgtc
      421 aatccccgaa cttcactgga aagaattgcg agaaaccttg ggctcgcctt tccgccttgc
      481 ccatttgaag aacctcgaag cctaaatcat cgccatccat cacctcggcc gcccgtctgc
      541 ggctccatct gattgacgta tacttatttt ggccacgtca tgagctcccc aaaacaatcg
      601 gttcaggaca tacagccggt tccccagatg gacctttcgg cgcaatacgc cgctatcggc
      661 gcggagattc gcgatgccgt caccagagtc ctggcctccc agcattttgt gctcggcaaa
      721 gagggagccg cgctcgaaca ggagattgca cagctctgcc gcgtgcgcca cggcatcggg
      781 gtcgcgtcgg ggacggacgc gctcatcctg gcgttgcgag ccgtcggcgt aaaagctggc
      841 gacgaggtca tccttccacc cttcacgttc gtcgccacgg gcagcgctgt cagcgcgctc
      901 ggcgcacggc caaaatttgc ggacatcgat cccgcgacat tcaacatcag cgtcgcgcag
      961 atcgaaaaac gcatcactcc acgtacccgc gccatcgtcg tggttcattt gtatggtttg
     1021 gctgcggata tggactccat ccgcgaagtc gccgcgaaac atcgcttgcc catcgtcgaa
     1081 gacaacgcgc aagccatcgg cgccacctac aaaggccgct ccaccggttc acttggcgac
     1141 atcgcctgct tcagctttta tcccacaaaa aatctgggcg cgtatggcga tgccggcatg
     1201 atcaccaccg actcgcccga actcgacgct cgtttgcgca cactgcgcaa tcacggccag
     1261 gcctcccgct ataacagcgt ggaacccggc tggaacagcc ggctcgatga aatccaggcc
     1321 gccatcttgc gcgtaaaact tcgccacctc aaagaatggg aacgcaggcg ccgcgctcac
     1381 gccgcgcaat acgcaaggct tctcgcgggc atcgccgaag tccagccgcc gcaagttccc
     1441 tcaacccgcg aacacgtctt ccaccaatac acgattcgcg cgcagcgccg cgacgagctg
     1501 cagaaatttc tcaccgatcg caagatcggc aacgccgtct actatccggt tccgcttcac
     1561 ctgcagccga tttatgcaaa cgaaggcttc cccgccggtt cccttcccga atcggaaaaa
     1621 gctgcggccg aagtggtatc gctgccgatg tttccagagc tccgcctgga gcaaatggag
     1681 cgcatctgct cagctatcac cgaattttac gggaggtaaa gactagatcc gccggaagtt
     1741 accgcccaaa atacagcagc gccagcaaga acacccacaa cccgtccata aagtgccagt
     1801 aatacgaagc cagttgggcg gccagcgacc ggctcacccg cgccgcgtcg aacttgtgca
     1861 gcgccacata caacagggcg catactccgc ccagcaagtg caccgcatgc agcgccgtaa
     1921 atacatagaa aaaactggtc gccaggcgcg atgtcacgta cacgccctgc tgagccagtt
     1981 gccaccatgc aatcacctgt ccaccgacga acagtatccc cagcgctgtg gtcaacgccc
     2041 acatccagcg aaagcccgca gcatcgtcca gccgcaattt ccgccgcgcc atttccagcg
     2101 tggcgctact caacagcaac acaacggtat tggcccaaag aattttgggt atccggatgc
     2161 cgatccaggt gtgcagatgc tgtacccgca gctccacaaa cgccgccgtc aacaccagaa
     2221 acaacatcac gatcgagacc atcccgatca gcacggcgat agaaaatttt ttgctgcccg
     2281 gcggccgcgg ttccggagcg tgctcgcccc cgcctccatc gtctccgccg ccgtcatcgt
     2341 cgccatcgcg cgaaggcggt ttccccccgc cgtcgccgtg gatctcttca ataatcagtt
     2401 caatgtcatc cgctacggtt cccggcatgg cccaacctca agctcgcaca tcagccgcca
     2461 acccagagtc tacacaaatt tgcggcggtg ggaactgccg aaaggaaggt acacgggggg
     2521 aacgccggaa gttaaggcag gaatggaaac cacgatggcg ggcaaaaaag ttggtgcggt
     2581 ggaaaggatt cgaaccttca cggtattgct accgccagcc cctcaagctg gtgcgtctgc
     2641 cagttccgcc accaccgcaa ttcgctgcaa gtcgccgagc tgcacggccg tgtgaggtca
     2701 cgccgcttct gattattctc cccttgccgt cgccaagcaa cagaaactct gccttgcctg
     2761 cctcggcaac ttatttccgc cgcagttact gtttcttcgg agactgcgcc ggtgaagccg
     2821 gagccggagt ctgttgcgaa ggtgctggcg cgctttgcgt ccccgccggc tgtgttgccg
     2881 gtgccgcagg cgtagtcgca ggagccgcgg gcttggtggc tgcaggcttt gtcgagttgt
     2941 aaatcaccga actgccgccg cccaccgagc gttcactgcg cagggccagc gccatcgcgc
     3001 acaccatgaa catcaccgcg caccacgtgg ttgcctggga aaggaccgtc gccgctccac
     3061 gcggcccaaa tgccgtctgg ctgccagcgc cgccgaatgc accggccaaa tccgccgctt
     3121 tgccgctctg cagcagcact acgatgatca gcaccaggca gttcagcaca aaaaatgtcg
     3181 ctagcagcca tcccgcccag cggaaaccca ctatgatgag cccaaatccg atcaggagcg
     3241 ccacgcccca tttcatccaa cctggcattg cctcacctcg aattttcttt gcatctggcc
     3301 gtcggcgcct gccgcgcggc ttacttcgtg ttcacaatcg tcgcgaacga tttcgcatcc
     3361 agactcgcgc cgcccaccag cgcgccgtcc agctcttcct gcgccatcaa tccctggatg
     3421 ttatcgggtt tgacgctgcc gccgtagagt atccgcacgc tggccgcggc ctccggcgaa
     3481 aactggtcgc gcacgcactg ccgcaagaac cgatgggctg ccgcagcaat ttccggcgtg
     3541 gccgtacggc ccgtcccgat tgcccagact ggctcgtatg ctaacaggat acgagagaat
     3601 tcctcacggg tcaacgcgcc cggaccaccg ataaactgct gagagagcac ttcttc
//
LOCUS       DASTPM010000042         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_636017, whole genome shotgun sequence.
ACCESSION   DASTPM010000042 DASTPM010000000
VERSION     DASTPM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636017"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VFR42_00695"
     CDS             <1..1676
                     /locus_tag="VFR42_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0367696.1"
                     /translation="FLPAHWSHANPIDILGDADPERYAKALEVAMADPGSDGLLAILA
                     PQGMTNPAEVAKHLAPYATGHNKPLLASWMGGEAIAEGERILSAAGIPTFSYPDTAAR
                     AFAYMWAYSEHLRGLYEVPALGDSLDGAEARRKKAEKLIGGVLATGRVLLTEAESKAL
                     LTLYNIPTVTTRVANTEEQAIAIAAETGYPVVAKLNSETVTHKTDVGGVRLNLASAEA
                     VREAYRAIENSVTVHAGRGAFQGVTIQPMIRTEGYELILGSSVDPVFGPVLLFGSGGQ
                     LVEVYRDRALAMPPLNATLAQRLLEQTKIHTALRGVRGRPAVDLVALRNVLVAFSQLV
                     LDLPRIAEIDINPLLASPEKIVALDARVVLAERKIPDEELPRPAIRAYPMQYVSRWTM
                     KSGAPVLIRPIRPEDEPAMVNFHKLLSDETVYLRYFHMAKLDTRIAHERLLRKCFIDY
                     DREMALVAEVPASDSEAAKIVGVGRLSRRPLSEEAELAVVVADRYQHQGIGSELIARL
                     VKIARAEKVKQIEAEFHSENSAIRHLAAHGGAKVERTSDPTCFRITVAL"
     gene            1955..>2171
                     /locus_tag="VFR42_00700"
     CDS             1955..>2171
                     /locus_tag="VFR42_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367697.1"
                     /translation="MKKPSVVIDGNEAAADVAYRLNEIIAIYPITPSSPMGEWSDQWQ
                     HEGRKNIWGAIPKVVEMQSEGGAAGTLH"
BASE COUNT          482 a          576 c          698 g          415 t
ORIGIN      
        1 aatttcttcc agcacactgg agccatgcga atccgatcga tatccttggc gatgcggatc
       61 cagaacgata tgccaaggcg ctggaagtgg ccatggccga tccgggcagc gatggacttc
      121 tggcgattct ggctccgcag gggatgacca atccagcgga ggtcgcaaag catttagcgc
      181 cttacgccac tgggcacaac aagccgttac tggcgagttg gatgggtggt gaagcaattg
      241 ccgaaggcga gcgaatcttg agcgccgcag gcatccccac attctcttat ccggatacgg
      301 cggcgcgggc gttcgcgtac atgtgggcat atagcgaaca cttgcgaggg ctgtacgaag
      361 tgcccgcact gggcgattcg ctcgacggcg cggaggcgcg tcgcaaaaaa gcggaaaagc
      421 tgatcggcgg cgttttggcc acaggccggg tacttctgac ggaggcggaa tcgaaggcgc
      481 ttttaacgct ctacaacatt ccgacggtga caacgcgtgt tgcgaataca gaggaacaag
      541 cgatcgccat cgctgcggag acaggttacc cggtggtcgc aaaactgaac tccgagacag
      601 tgacacacaa gacggatgtc ggaggagtca ggctaaatct tgcgagcgcc gaagcggtgc
      661 gagaggcgta ccgcgcgatc gagaattcgg tgacagtgca tgccggccgt ggcgcgtttc
      721 agggcgtgac catccagccg atgattcgaa cggagggcta tgaactgatc ctcggcagca
      781 gcgtagaccc ggtgtttggc ccagtgctgc tgtttgggtc gggaggccaa ctggtggaag
      841 tgtaccggga ccgcgcgctc gctatgccgc cgttgaatgc cacgctcgcg cagcggttgc
      901 tggagcaaac caagattcat accgcgctgc gaggggttcg ggggcggccg gcggtcgatc
      961 tggtcgcact ccggaatgtt ctcgtagcat tcagccagtt ggttctggat ctgccgcgca
     1021 tcgcggaaat agatatcaac ccgcttttgg cgtcccctga gaaaatcgtc gctttggacg
     1081 cgcgagtagt gcttgcggag cggaaaattc cggatgagga gttaccgcga cccgcgattc
     1141 gggcgtatcc gatgcagtat gtttcgcggt ggacgatgaa gagcggggcg ccggtgctca
     1201 ttcgcccgat tcgccccgag gacgaaccgg cgatggtgaa ttttcacaag ctgctatcgg
     1261 acgagaccgt gtatctgcgc tatttccaca tggcaaaact cgatacgcgg atcgcgcacg
     1321 aacgtttgct gcgcaaatgt ttcattgact acgaccgcga gatggcactg gttgcggaag
     1381 taccagcttc tgattctgaa gcggcaaaga ttgtgggcgt gggccggctg tcgcggcgtc
     1441 cattgtcaga agaggctgag cttgcagtgg tggtggcgga ccgctaccag catcaaggaa
     1501 taggcagcga actgatcgcg cggctggtga agattgcacg cgcggagaag gtgaaacaga
     1561 tcgaggctga attccattca gagaatagcg cgattcggca cctggccgct catggcggag
     1621 ccaaggtgga gcgcacttcc gatcctacgt gcttccgcat cacggtagcg ctgtagatga
     1681 cccgatgtag tgccattggt ccaaaacggg acagtcgtac accttccggc cgctaatcaa
     1741 gccagccttt taagtagaat tttagggagc attgtgacgc ccgtcacagt gggcagtgtc
     1801 acagcccaca gccgcgatgt tcgcggtctg ctaaaaaaag agacagcacg tatgccaggc
     1861 ccaagcgcag gcgatcaagg tgctgccggt aacccctcct tccgaggctg gcaatgatct
     1921 cgcacgcttc tcatcagcgg tagaagagaa cacaatgaaa aagcccagcg tagttatcga
     1981 cggaaacgaa gccgcggcag acgttgcgta tcggctgaac gagataatcg ccatctatcc
     2041 gattacgcct tcgtcgccca tgggcgaatg gtcggatcaa tggcagcacg aagggcggaa
     2101 aaatatctgg ggcgccattc ccaaggttgt ggaaatgcag tccgaagggg gcgcggcggg
     2161 cacgttgcac g
//
LOCUS       DASTPM010000043        10098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_751310, whole genome shotgun sequence.
ACCESSION   DASTPM010000043 DASTPM010000000
VERSION     DASTPM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751310"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFR42_00705"
     CDS             complement(<1..494)
                     /locus_tag="VFR42_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367698.1"
                     /translation="MSNSVGTQIARSFDWLVCKTAGITFDTIQYFNKRNPNPSVTPQW
                     SDKPLLKSWEKTKPTLGFPRQTDSLCPGCVKEAREAIIAGKKDWRDLMHEKVGEIKAQ
                     IVERDGQIWMLKDCPIHGHYEDMMAIDSKFLDWIEKQFPGRDIPAHNDEDLHKHGSST
                     VRYGR"
     gene            complement(900..1949)
                     /locus_tag="VFR42_00710"
     CDS             complement(900..1949)
                     /locus_tag="VFR42_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367699.1"
                     /translation="MADWKQITARIRRARGSKDPAGQLTLLFEKTKDAMVAFELGRHF
                     EIVRNPAQALEWYQNAYSRFRRGDWKTKCGEAIARLGGELPVDLAQKSSNESAAAVTA
                     PEPALPFEQNATAFESPIAVGAENPPSDAPSTAASEAEKKRRRRGRRGGRHRGNKASV
                     LPTSESPAPSPREHHTRREVAAGPPADIVPPRAVDSRAIPRFPVEPLPDSSAPGVKGR
                     SGDPGLSSRLSLLEMQLRRLLTCPPAKLDHAEHAPAGPGVFVLTDDDMTTYYHVETCD
                     TLRIAIGTLARTGSTRRGNPNLRPLLADHLGIAEARVSKYLVDHCVVRWLQLDEGAPQ
                     FAHFAIAVLRPILNE"
     gene            2098..2793
                     /locus_tag="VFR42_00715"
     CDS             2098..2793
                     /locus_tag="VFR42_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367700.1"
                     /translation="MFRPQAVLAVVVSLSLLVLPTYGAPASAIGTVTSASGARIGATT
                     ASVGSTVFVGDHLSTEKLGSIQVRAGAARLLLSGASAVDVAQLQGTTPAARLMNGTVV
                     FSTANAKAFVLHASKADIRAKTDEPTVGQITYVNDKELRVRCNRGALAITVEDETQFV
                     PEGMSYRVILDPDSYYAAEASAAQGPQGAGGRGGRPPLRAGRSRFLLIAIIITGVATG
                     VALYEALQSPDKP"
     gene            complement(2933..3310)
                     /locus_tag="VFR42_00720"
     CDS             complement(2933..3310)
                     /locus_tag="VFR42_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0367701.1"
                     /translation="MKDVVLTEKGPKPIGPYSQAIKSGGFLFASGQVALDPRSNEFLA
                     GDIRQQTERVLENVKAILEAGGANLHQVAKTTVFLKDMNDFAAMNEVYGKYFTSAPPA
                     RSTVQVARLPKDALVEIEVIASL"
     gene            complement(3307..4791)
                     /locus_tag="VFR42_00725"
     CDS             complement(3307..4791)
                     /locus_tag="VFR42_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367702.1"
                     /translation="MLLSRSTSFAAAHLFALAACAAGWLAGCAAPLGPAYVVQQQEIR
                     VDFSPQPQPEIRIAAEYRLQNTGNQPLDSLDVRLPGRRFRPHSMQALWDTAPIATNVS
                     PDNARDTELHFSQLWKIGETHTLKLSYEIPSASAGEDSLGFSADAFYLPAEGWTPQLP
                     QARGVFGSGGVPPKQWPLTVSVPQRFFVHASGGKPKRSAKGESAEYRFAQTSEDLNPF
                     VVAGAYLESQQTLAHNQKIRLWTRGKPDSTQLRDSGESLSHILATYDALFGSRGPSNP
                     PLWIVECPAPTGCISQRETTYSALLYGHQTARSAEMISRDCVIVDPRLAAGSLEASAG
                     PAFAAGWLGYGQNPGFYEQQPPVSALPAFAAALAREATSGPGVRTEIIQRALAAVPEP
                     APPASNSDPAVTRAKSLLLFFALRDRIGPDAFRKAVQHMLYARRGRGFDVTDLIAALE
                     QESHQPAGPFVRQWIKSPGVPAEFRSRYSQAASQQQIFLQETTR"
     gene            complement(4856..5467)
                     /locus_tag="VFR42_00730"
     CDS             complement(4856..5467)
                     /locus_tag="VFR42_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367703.1"
                     /translation="MRRPSYLPALLLLPELLAGCKSQPFTDYRPLDKAGMWSSSLQDL
                     KKLNVSDTEIAQLVALKNAGVNDDMCVSLVQAAHDHQHPFVSAASAASLNRAGFSDEQ
                     ILAIARNDKLDVLSGDAVMLRLIGLSDPTIQLILQRRMRGLTTMSSAEIGRLKNTGLS
                     EKEIVARMQSGMTDAQADAEAAAREKARAHSGTGFVRVHGRRR"
     gene            complement(5560..7779)
                     /locus_tag="VFR42_00735"
     CDS             complement(5560..7779)
                     /locus_tag="VFR42_00735"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU0367704.1"
                     /translation="MSGSSHLPLQFNRDRTERQLDARLAELLDKLKRNRPSDDPWLVR
                     RAYDIAAERHRDQLRRSGDPYLTHLLEVAHILADMRMDATTLSAALLHDVIEDTEFPI
                     SRIQERFGEETARLVEGVTKIGRLSMPTPETRQAENVRKMVLAMVNDVRVVVVKLADR
                     LHNMRTIEFLEPAKQQRIARETLEIYAPIAHRLGMGVIRNELEDLSFRVLEPESFLGL
                     QKEVTEQQPVHQRFLEEVQATIRSKLVENGIPAELEARVKGLYSLHRKVIRQQRGLEQ
                     VYDLLAVRVITDNERNCYAALGVVHHIWRPVPGRFKDYIAMPRPNLYQSLHTTVIHTG
                     QAFEVQIRTQEMHRIAEEGVAAHWKYKDGKPAGDDDQRITWMRQLIEWSQELQEPGEF
                     LTTLKVDLTPNEVYAFTPKGRVLELPAGATPVDFAYSVHTEVGHQCVGAKINGQMVTL
                     RHEISSGDVVEILTQKGHGPSRDWLSFVKSSGAKSKIRHWINVHERAEATEMGRRLLE
                     KESRAFGRSLKKIEEAELQKAAADYGLSKVEDLFAAVGFGKYSARQVLTKVVGEGVKP
                     AEVVDDTKPTLVKTVKRMLGIGEAPLVVKGHDDLLVFRAKCCNPIPGDEIVGYITRGR
                     GVAVHTRLCPNVQNLLYQAERRIAVEWGGASAATFPVQLAIRAKDRAGLLAEITAVIS
                     GAGSNIRNLESRPDRLNARIEASLEIVDRRQLESILANIRKISGVHGVERVYQPAGE"
     gene            complement(7907..8785)
                     /locus_tag="VFR42_00740"
     CDS             complement(7907..8785)
                     /locus_tag="VFR42_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367705.1"
                     /translation="MDASMTHAPVANTALPRSASSFRQAAAFLTLLLAVAGFSGCDKL
                     KARDLLNKGVQSFKNGQYDAAVEDFKQAKDLDPSLLNARLYLATAYASQYIPGAPSEQ
                     NRNIGKQAVEEFKNILQTNPDNLSAIDGIGSILFQMAGTPYDPKLFEESKTYHQKHIQ
                     LKPEDPEPYYWIGVIDWTLAFRANGEMRRDYNEKNIRKQVRDTDPLPPALRAEYLAKY
                     GPLVDEGIADLQKAISLRPDYDDAMAYLNLLYRRKADTVESATERASYQKQADDLVDK
                     VKEVKQKRASQPQPTT"
     gene            complement(8832..9248)
                     /locus_tag="VFR42_00745"
     CDS             complement(8832..9248)
                     /locus_tag="VFR42_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0367706.1"
                     /translation="MAMQVGGKGGAFAEMNVVPLIDILLVLLVIFMIIPHEQKGLVAQ
                     IPQPAPPTSPALPDEGVIVVQVQNSGELRINGQPVTWDELGNRLEAIFKLRARKVAFI
                     RGDALVEFALVARIVDVMHGSGISVGLLTPGLEQSR"
     gene            complement(9416..9838)
                     /locus_tag="VFR42_00750"
     CDS             complement(9416..9838)
                     /locus_tag="VFR42_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0367707.1"
                     /translation="MSVGESKGGAIAEMNVVPLIDILLVLLIIFMVITPLTPKGMDTL
                     VPQPNPNQQQNQDLENKTVVVQITKDGKVKINNEDSTWDGLGPRIEQIFKERAEKVAF
                     VKGDNEVLFQEVARAIDIMRSAGIDKVGLITAKLEAGQ"
BASE COUNT         2055 a         2706 c         3227 g         2110 t
ORIGIN      
        1 cggccgtagc gaacggtgga agagccgtgc ttgtggagat cttcgtcgtt atgcgcggga
       61 atgtcgcggc cggggaactg cttctcgatc cagtcgagaa acttggagtc gatggccatc
      121 atgtcttcgt agtggccgtg aatcgggcaa tccttgagca tccagatctg gccgtcgcgc
      181 tcgacgattt gggccttaat ttcgccgacc ttttcgtgca tcaggtcgcg ccagtctttt
      241 ttgccggcga tgatggcttc gcgggcttcc ttgacgcagc cgggacagag tgagtctgtt
      301 tggcgcggga agccgagggt gggcttggtc ttttcccaag acttgagcag cggcttatcg
      361 gaccactgcg gggtgacgga aggattcgga ttacgcttat tgaaatactg aatggtgtcg
      421 aacgtgatgc ctgcggtctt gcacacgagc caatcaaagg accttgcgat ctgcgtgcct
      481 actgagttcg acataaacct ctatcctcca cttttcaaaa cggcactttt gaaatgcgcg
      541 tccagcgatg gactcgcgaa gagccggtta aagcaaaaaa gtttgatgcg aaaggcagcc
      601 tgttatgagc tcaggatttg taaaaccact tctttacttc aggcttgcaa aacaagcact
      661 gaaaccccga aacccgttgg aatgccccca gcaggctact ttctaaccat aacacgccct
      721 gcgttatgga ctaagtcttt cggcttgtat agtttgcagg agggccgggg cggtgggaag
      781 gaagttgcac cggacggcga aaaagggggg ttgagcggca aaatcggggg ttaagcggat
      841 ggttgggaag gccgcaaaag gggtaacgaa tagggtgcaa aatgggaatc tctctgcagc
      901 tattcgttga ggatagggcg aagaacggcg atggcgaagt gggcgaactg cggagcacct
      961 tcgtcaagct gcagccagcg gacgacgcaa tggtcgacaa gatatttgga gacgcgcgcc
     1021 tcggcgatgc cgaggtgatc ggcgagcagg ggtctcaaat tgggattccc gcggcgcgtg
     1081 gagccggtgc gcgcgagcgt gccgatcgct atgcgcaaag tgtcgcaagt ctctacatga
     1141 tagtaggtag tcatgtcgtc gtcggtgagc acgaagacac ccggcccggc tggagcgtgc
     1201 tccgcgtgat caagttttgc cgggggacag gtgagcaaac gccggagctg catttcgaga
     1261 aggctgagtc gcgaagaaag gcccgggtcg ccggaacgtc ctttgacgcc gggggcagaa
     1321 gaatccggca acggttcgac gggaaaacgc gggatggctc gcgaatcgac cgcgcgcgga
     1381 gggacgatat cggcgggagg cccggcagcg acttcgcgac gggtgtggtg ttcgcgcggt
     1441 gaaggcgccg gagattcgga agttggcagc acggaagctt tgttgccgcg atggcggccc
     1501 ccgcgacggc cacggcggcg gcgttttttt tcggcttcgc tggccgctgt cgaaggagcg
     1561 tcggagggag gattttccgc gccgaccgcg atgggcgatt cgaatgcggt ggcattttgt
     1621 tcgaaaggta gtgcaggctc gggagccgtt acggccgcag ccgattcgtt actggatttc
     1681 tgcgcaagat ctacaggaag ttccccgccc aggcgcgcga tagcttcgcc acacttggtt
     1741 ttccagtcgc cgcgacggaa gcgggaatag gcgttctgat accactcgag agcttgcgct
     1801 ggattgcgca cgatttcgaa atggcgcccg agctcgaagg caaccatggc gtccttggtc
     1861 ttctcaaaga gaagagtgag ttgcccagca ggatctttac ttccgcgagc gcggcgaatg
     1921 cgcgcagtga tttgtttcca gtcggccatt gtcaacgcat tgtagcagcg gtaggcatac
     1981 cagcatcgtg aaaatacata cactagattc gactctaaga accttatggg gcgaatagcg
     2041 attgacggtt gcaaaagccg tgggtattct ggttgcggcc ctctcacgga ggcggtgatg
     2101 tttcgcccac aagcagtgct tgctgttgtg gtgtctcttt cgctccttgt gttgccgacc
     2161 tatggcgccc ccgcctcagc catcggaacg gtgacatcag cgtcgggtgc gcgtattggc
     2221 gccacgacag cgtcggttgg ttcgacggtt ttcgtcggag accatctcag cacggaaaaa
     2281 ttaggaagca ttcaagtgcg cgcgggcgcc gcgcgcttgc tgctctctgg cgcaagcgcg
     2341 gtagacgtgg cgcaactgca aggcacaacg ccagctgcgc gattgatgaa tggcaccgtg
     2401 gttttttcga cggctaacgc caaggcattt gtgttgcacg catcgaaagc ggacatccgt
     2461 gcgaagaccg atgagcccac agtcggtcaa attacttatg ttaacgacaa ggaattgcga
     2521 gtgcgctgca accgcggcgc gctggccatc actgtcgagg acgaaacaca gtttgttccg
     2581 gaaggaatgt cctatcgggt gattctcgat ccggattctt actacgcggc ggaagcttcg
     2641 gcggcgcaag ggccgcaggg cgcgggtggg cggggcggca gaccgccgtt gcgcgcgggc
     2701 cgcagccgtt ttctgctgat tgccatcatc atcacgggag tagctactgg cgttgcgttg
     2761 tatgaagcgc tgcagagccc ggacaaaccg tagaaacagt tcaaaattta gagttaaaag
     2821 ttcaaagtaa accttccagg ccaagtctcg gaagctgttc gcgtgcgttt gcgaaaaatc
     2881 gtacgtgaac gacgctcaac agatctccga gacgcggtgg cacggcctcg cgctagagtg
     2941 acgcaatgac ttcgatttcg acgagggcgt ctttggggag ccgcgcgacc tgcacggtgg
     3001 aacgggcggg cggtgcggaa gtgaaatact tgccgtagac ttcgttcatc gccgcaaaat
     3061 cattcatatc cttcaggaag acggtggttt tcgccacctg gtgcagattg gcgccgcccg
     3121 cttcgagaat cgccttgacg ttttccagaa ctcgttcggt ttgctggcga atatcgcccg
     3181 cgagaaattc gttggagcgc ggatcaagcg cgacctggcc cgaggcgaaa agaaatccgc
     3241 cggatttgat ggcctgggaa taagggccga ttggcttcgg gcctttctcg gtaagcacca
     3301 cgtctttcat cgtgtcgtct cctgtaagaa aatttgttgc tgcgaagcgg cttgcgaata
     3361 gcgactgcga aattcagcgg gaacaccggg acttttgatc cattggcgga caaagggacc
     3421 ggcgggttgg tgcgattcct gctccagggc ggcaatcagg tcagtgacat cgaaaccccg
     3481 gccgcggcgg gcatagagca tgtgttgaac ggccttccgg aaggcatccg gaccaattcg
     3541 atcgcgtagc gcaaagaaca gcagcaggct tttggcgcgt gtgacggccg ggtcggaatt
     3601 cgatgcgggc ggcgcgggct cgggaaccgc ggcgagcgcg cgctgaatga tctcagttcg
     3661 aactcccggg cccgaggtcg cttcgcgggc gagggctgcg gcaaaggccg gcaacgccga
     3721 gacaggcggt tgctgttcgt agaacccggg attttggccg tagcctaacc agccggccgc
     3781 gaacgcagga cccgcggaag cttccagcga gcctgcagca aggcgcggat caacgatcac
     3841 gcagtcgcgg gaaatcattt ccgcggaacg cgctgtttga tgtccgtaga gcaaagctga
     3901 ataggtggtt tctcgttgcg agatgcaccc ggttggcgcg ggacactcca caatccataa
     3961 gggaggatta gaagggccgc gcgaaccgaa cagggcgtca tacgtggcaa ggatgtgcga
     4021 caacgattcg cccgaatcac gcaactgggt ggagtcgggt ttgccgcgcg tccacaagcg
     4081 aatcttttga ttatgggcaa gggtctgctg cgattcgaga taagcgcccg ccacgacaaa
     4141 cggattcaaa tcctcgctgg tctgggcgaa gcggtattcg gcggattcgc cttttgcgga
     4201 tcgctttggc ttgcctccgc tggcgtgtac gaaaaatctc tgcggcacgg agacggtcaa
     4261 cggccattgt tttggcggca cgccgccaga accaaaaacg ccgcgcgcct gcggcaactg
     4321 tggagtccag ccttcggccg gaaggtaaaa cgcatctgca gagaaaccga gcgaatcttc
     4381 gccggcggaa gcggaaggaa tttcatagga aagtttgagc gtgtgagttt cgccgatttt
     4441 ccaaagctgg gaaaagtgca gctcggtatc gcgagcgtta tccggcgaaa catttgtcgc
     4501 aatgggagct gtgtcccaca aagcttgcat ggaatgcggc cggaagcgcc gtccgggcag
     4561 gcggacgtcc agggaatcga gcggctggtt gccggtattc tgcaagcgat attcggcggc
     4621 aatgcgaatc tccggctgag gctgcggaga aaaatcgacg cgaatttcct gctgctggac
     4681 cacataggcg ggcccaaggg gcgcagcgca acccgcaagc cagcccgcgg cgcaagcggc
     4741 aagagcgaac aggtgagcag ccgcaaacga agttgaacga gaaagcagca caggttgatt
     4801 gtagccacgc tgggaaagag cgcaagcgag gttgccgggg cacagccgcg atgaattagc
     4861 ggcggcggcc gtgcacacga acgaagccgg tgccggaatg cgcgcgggct ttttcgcgcg
     4921 ccgcagcttc ggcgtcggcc tgggcatcgg tcatgccact ttgcatgcgc gccacgattt
     4981 ccttttcgct gaggccggtg tttttcaagc ggccgatttc ggcgctggac atggtcgtga
     5041 ggccccgcat gcggcgctgc aagatcagct gaatcgtcgg atcggagagt ccgattagac
     5101 gcagcatgac ggcatcgccg ctcagcacat cgagcttgtc attgcgcgcg atggccagaa
     5161 tctgttcgtc gctaaagccg gcacgattga ggctggcggc agaagcagcg ctgacgaagg
     5221 gatgctggtg atcatgggcg gcttgcacca gcgaaacaca catatcgtca ttcacaccgg
     5281 catttttcaa cgcgacgagt tgcgcaattt ccgtgtcgct gacgttgagc ttcttcaggt
     5341 cttgcaggct gctggaccac atgccggctt tgtcgagagg acggtaatct gtgaacggct
     5401 ggctcttgca tccggcaaga agctccggca gaagcaggag tgcgggaaga tatgacggtc
     5461 ttctcatgct tctatcttat tggttacggc gtgagagggc acgaaatgtg ctgtggccga
     5521 tacccgcccg aaaggacagg cttcagattg gagagggctt cattcgccgg cgggttgata
     5581 gacgcgctcc acaccgtgta cgccggagat tttgcggatg ttcgcgagaa tgctttcgag
     5641 ctggcggcga tccacaattt caaggctggc ctcgatgcgg gcgttgaggc ggtcagggcg
     5701 gctttccagg ttgcgaatgt tggagccggc cccgctgatg acggcggtga tttcagcaag
     5761 cagtccggca cgatccttgg cgcggatggc cagctgcacc gggaaggtgg cggcgcttgc
     5821 gccaccccat tcgaccgcga tgcggcgctc cgcctgatag agaagattct ggacgttggg
     5881 gcaaaggcgg gtgtgcacgg caacgccgcg tccgcgtgtg atgtagccaa caatttcgtc
     5941 gcccggaatg ggattgcaac atttggcgcg gaaaaccagc agatcgtcgt gacctttcac
     6001 gacaagcggg gcttcgccga tgcccagcat gcgtttgacg gtcttgacca gcgtgggctt
     6061 ggtgtcgtcc accacttcgg cgggcttgac gccttcgccg acgacctttg tgagcacctg
     6121 gcgcgcggaa tatttgccga agcccacggc ggcgaacaga tcctcgactt tggacaggcc
     6181 gtagtcggca gcggctttct gcaattcggc ttcttcgatt tttttcaggc tgcggccgaa
     6241 ggcgcgcgat tccttttcga gcagtcggcg gcccatttcc gtggcttccg cccgttcatg
     6301 aacattgatc cagtggcgga ttttgctttt ggcgccggaa gatttgacga agctgagcca
     6361 atcacggctg ggcccgtggc ccttttgcgt gaggatttcg acgacgtcac cactggaaat
     6421 ttcatggcgc agcgtaacca tctggccgtt gatttttgcg ccgacacatt ggtggccgac
     6481 ttcagtatgg acggagtaag cgaaatccac cggggtggcg ccggcgggaa gctccagcac
     6541 gcggcccttg ggcgtaaaag cgtagacttc attgggagtg agatcgactt tgagggtggt
     6601 gaggaattcg ccgggctcct gcaattcctg cgaccactcg attaactggc gcatccaggt
     6661 gatgcgctgg tcgtcgtcgc cggcgggctt gccatccttg tatttccagt gggcagcgac
     6721 gccttcttct gcgatgcggt gcatctcctg cgtgcggatc tgaacttcga aggcctgccc
     6781 cgtatgaatg acggtggtgt gcagtgattg ataaaggtta ggccgcggca tggcgatgta
     6841 gtccttgaag cggccaggga ccgggcgcca gatgtggtgg actacgccga gggcggcgta
     6901 acagttgcgt tcgttatcag tgatgacgcg gacggcgagc aggtcataca cctgttcgag
     6961 gccgcgctgc tggcgaatga ccttgcggtg caaggaatac aagcccttta cgcgggcttc
     7021 gagttcggcg ggaatgccgt tttcgaccag cttggagcga atggtagcct ggacttcttc
     7081 cagaaaccgt tgatggaccg gctgctgttc ggtgacttct ttctgcaggc cgagaaacga
     7141 ttccggctcc aacacgcgaa aggaaagatc ttccaattca ttgcgaatta cgcccatacc
     7201 aaggcggtga gcaataggcg cgtagatctc aagagtttcg cgagcgatgc gctgctgctt
     7261 ggccggctcc aggaattcga tggtgcgcat gttgtgcagg cgatcagcga gcttgaccac
     7321 cacgacgcgg acgtcgttga ccatggccag gaccatcttg cgcacatttt cggcctggcg
     7381 cgtttccggg gtgggcatgc taagccggcc gattttagtt acgccttcaa ccaaccgggc
     7441 ggtttcttcg ccgaagcgtt cctgaatgcg cgagatggga aattccgtgt cctcgatgac
     7501 gtcgtggagc agcgcggcgg atagcgttgt cgcgtccatg cgcatgtcgg caaggatatg
     7561 cgcgacttcg agcaggtggg tgagataggg atcgccggag cggcgaagct ggtcgcggtg
     7621 acgctcggcg gcgatgtcgt aggcgcgtcg aaccagccag gggtcgtcgg aggggcggtt
     7681 ccttttgagc ttgtccagta gctcagccag gcgcgcgtcg agctgccgct cggtgcggtc
     7741 gcggttgaat tgcaagggaa gatgggacga cccggacata tttgaatagt ttacgcccga
     7801 aggcaaataa gggcgagccg agcggcgacc tgcgcggacg tgccgcaaaa acgaaacggg
     7861 gcaccccagg atgccccgct taaagatggc tgatttcgta actctatcag gttgtgggct
     7921 gcggttgcga agcgcgcttc tgcttcactt ccttaacctt gtcgaccagg tcgtcggctt
     7981 gcttctggta gctggcacgc tcagttgcgc tctcgacggt atcggccttg cggcgataga
     8041 gaaggttaag gtaagccatg gcgtcgtcat agtcaggacg cagagaaatt gctttttgga
     8101 ggtcggcgat gccttcgtca accagcgggc cgtatttggc caagtattcc gcacgaagcg
     8161 ccggaggcaa cggatcggta tccctgacct gcttgcggat atttttctcg ttgtagtccc
     8221 tgcgcatttc gccgttggca cggaacgcga gcgtccagtc aattacgccg atccagtaat
     8281 aaggttcggg atcttcgggc ttcagctgaa tatgcttctg gtggtaagtc ttggattcct
     8341 caaaaagctt ggggtcgtat ggagtaccgg ccatctggaa aaggatggag ccgatgccgt
     8401 cgatggcgct gagattgtcc ggattggttt gcaggatgtt cttgaattcc tcgaccgcct
     8461 gtttgccgat gttgcgattc tgctcggagg gagcgcccgg aatgtactgg ctggcgtaag
     8521 cagtggcgag atacaagcgg gcgttgagga gtgaaggatc taggtcctta gcctgcttga
     8581 aatcctcaac ggctgcgtcg tattggccgt tcttgaagga ctgaacgccc ttgttgagta
     8641 gatcgcgcgc cttcaacttg tcgcaaccgg agaaaccggc gacggccagc agcagcgtca
     8701 agaacgccgc agcctggcgg aaagaagatg cggaccgcgg aagggccgtg tttgcaaccg
     8761 gtgcatgagt catcgaagcg tccatcccca cgagaagtca gattcgccct gcgcgggcta
     8821 ttccatcaca atcaacggct ttgttccagt cccggagtca acaatccaac ggaaattccc
     8881 gagccgtgca taacgtccac gatgcgggcg accagcgcga actccaccag agcatcaccg
     8941 cgaatgaacg caaccttcct ggcgcgtaac ttgaaaatgg cctccaggcg atttcccagc
     9001 tcgtcccagg taaccggctg gccgttgatg cgcaactcgc cggagttctg cacttgcacg
     9061 actataacgc cttcatctgg gagcgcgggc gaagtcggcg gtgcgggctg aggtatctga
     9121 gccaccagcc ctttctgctc gtgcggaatg atcataaaga tcaccagcag aaccagcaga
     9181 atatcgatca ggggaactac gttcatttcg gcgaacgcgc cacccttacc accaacctgc
     9241 attgccataa tccacctcgc aagaaagagg tcaggatttg cactgacaaa ccctgagacc
     9301 aactaccccg gcgaggagga ctctcacaat ccctgtttat tagggttcgt aagacaaccc
     9361 taagaccctg gcggaaaaac aaaatggccg cgagcggccg gcggacccgg cttagttact
     9421 ggcccgcttc gagcttggcg gtgatcaggc caaccttgtc gattccggcg gagcgcatta
     9481 tgtcgatggc gcgcgccact tcctggaaga gcacttcgtt gtcgcctttg acgaaagcga
     9541 ccttttccgc gcgttcttta aagatctgtt cgatgcgcgg cccgaggcca tcccaagtgg
     9601 aatcttcgtt gttgatcttg accttgccgt ctttggtgat ctgcacgaca accgtcttgt
     9661 tttccaggtc ctggttctgc tgctggttcg gattgggctg cggaaccagc gtgtccatgc
     9721 ccttgggcgt cagcggcgtg atcaccatga aaataatcaa cagcacgagc agaatgtcga
     9781 ttagcggaac gacgttcatt tccgcaatcg caccgccctt gctttcacct acgctcatcc
     9841 ccattgcgag tctcctgtca gctcgtccat gtagctcggg gtttgtaaaa caaaccctga
     9901 aaccctgcga agggccgaag ctgttgcggc gcgccgggcg ggtgccagcg cgacccgcgg
     9961 aacttcttta actcagggtt tgcaagtaca aaccctgaaa cccgaaacga atggtaacca
    10021 gcagccgcaa cctcagtcgc cgttaggggg cggaggaggc ggtgttcctg ttctctgatt
    10081 gcgttcggtc agcaggcc
//
LOCUS       DASTPM010000044         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1097429, whole genome shotgun sequence.
ACCESSION   DASTPM010000044 DASTPM010000000
VERSION     DASTPM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097429"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFR42_00755"
     CDS             <1..316
                     /locus_tag="VFR42_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367708.1"
                     /translation="ATLGSGAPYLWRHSLYEFRKYRPQVSVKDYVGNYSFASCNSSVG
                     GAAALTIHLPSPLEFVILVAISFAIELSEDSNLQGSRLPFALLLAAAAARTFWICDVR
                     AP"
     gene            313..531
                     /locus_tag="VFR42_00760"
     CDS             313..531
                     /locus_tag="VFR42_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367709.1"
                     /translation="MNAHSGHRANSAVRGKTHHSFHFLPAIPRDYLARRRMFRNISNV
                     LMIVLLSLIIAGVIIYTSDPNSFSFLRH"
     gene            complement(708..1583)
                     /locus_tag="VFR42_00765"
     CDS             complement(708..1583)
                     /locus_tag="VFR42_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU0367710.1"
                     /translation="MNPCAGGGNAGRKIADTRSEFARRNFAVRFVASRSAEEFRTEVQ
                     AAVAQGCKTLVAMGGDGTLQLLVREAIGHEVIIGVMPAGGGNDFAAALGIRNWKQAVL
                     AIVHGKTRPVDIARATFANGDKAEYLGGGGVGLDAEAARHASGKFLGWPGRLRYLASA
                     IAALRAYAGVNVEIEFPETDPPALRKRVLLAAALNTPSYGGRVRLAPDARLDDGELDF
                     VILEMLSRLEVAKLILQLLLTGEFGTRHVTRVRATRARISTEEGSLFHGDGELLGNTP
                     VQIEVLPQAIRMFVP"
     gene            complement(1698..>2735)
                     /locus_tag="VFR42_00770"
     CDS             complement(1698..>2735)
                     /locus_tag="VFR42_00770"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0367711.1"
                     /translation="FGLTALCIAANRGYEEEGPIRDRVKATLGFALKSVQHQHGFLYH
                     FVNGETGQRILNSEVSPVDTCLFLCGELTCKGFFDDNEIQQLATELYERADWNWMLHS
                     GQTLAMGWMPEQGFLRARWDSYSELMMMYLLGMASRTHPLPPTAWNSWQRPQFEFNQI
                     AYIGAHAPIFAHQYSHAWFNFRGIHDKYGDYFANSIVASKIHKLWCLELAERFSDYSE
                     NLWGISASDSEHGYAIWGGPPAMGRIDGSIVPCASAGSLVFLPKECVSVLQNIREKFG
                     KRVWKKYGYVDAFNPLNNWSSPDALGIDAGITIIMAENARSSFVWEQFAKNPEVSKGL
                     ELAGFQPNAKS"
BASE COUNT          521 a          900 c          669 g          645 t
ORIGIN      
        1 tgccaccctg ggctccggag cgccctatct ttggcgtcat tcgctatatg agtttcgaaa
       61 ataccgcccg caagtttccg tcaaggacta cgttggcaac tactcgttcg catcatgcaa
      121 ctcgtcagtg ggtggcgcgg ccgccttaac aattcatctc ccatcgcctc ttgaatttgt
      181 tattctagtc gccatcagtt ttgctatcga gctgtccgag gatagtaacc tgcagggatc
      241 ccgcctcccg ttcgcactgc tcctggccgc cgctgcagct cgaactttct ggatttgtga
      301 cgtgagggct ccatgaacgc tcactctggc catcgagcga acagcgcggt tcgtggcaag
      361 acgcaccatt cttttcattt tctcccagcg attccccgtg actatctcgc ccgccgccgt
      421 atgttccgaa atatctctaa cgttctcatg atcgtgctct taagcctgat catcgctggt
      481 gtgatcatct atacgtccga ccccaattcg ttctccttcc tgcgtcactg atttcggagt
      541 cggttgaatt cttgcgccac cgacccgcct cgatgccgcg cgaattcggt ttggggccag
      601 ctttttttgt gcggtaggct cgtcggattt tggtggcaca gacttcagcc tattcctgcc
      661 gaagtttgtc gcggcgcgcg ccattcatcc gttcgcgccc gctgttgcta aggaacgaac
      721 attcgaatcg cctgcggcaa aacctcaatc tgtacaggcg tatttcccaa tagctcgccg
      781 tctccatgaa acaggcttcc ttcttccgtc gagatcctcg ccctcgtcgc tctaactcta
      841 gttacgtgcc gtgttccaaa ctcgccggtg agcaacagct gcagaatcag ctttgccact
      901 tccaggcgcg acagcatttc gaggatcaca aaatccagtt cgccatcgtc cagacgagcg
      961 tcgggcgcaa gccgcactct gccgccataa cttggcgtgt tcagggcggc ggccagcaac
     1021 actcgttttc gcaacgctgg cggatcagtc tcgggaaact caatctccac atttactccc
     1081 gcgtacgcgc gcaacgccgc gatcgcgctc gccagatacc gaagccttcc gggccatccc
     1141 agaaacttgc cgctcgcatg tcgcgctgcc tcggcgtcca gcccgactcc tcctccgccc
     1201 agatattcgg ccttgtcgcc atttgcaaat gtcgctctcg cgatgtccac cgggcgcgtc
     1261 ttcccatgca caatcgcgag taccgcctgt ttccagttcc gtattcccag cgccgcagca
     1321 aagtcattgc cgccgccagc gggcattacg ccgatgatca cctcatgacc gatcgcctcg
     1381 cgcaccagca attgcaaggt gccgtccccg cccatcgcaa ccagcgtctt gcagccttgt
     1441 gcgaccgccg cctgcacttc cgttcgaaat tcttctgcgg accgcgatgc aacaaatcgc
     1501 accgcaaagt ttcgccgtgc gaactccgat cgtgtatccg cgatcttgcg tcctgcattg
     1561 ccgccgccgg cacacggatt cacgaaaact gtcgcgttcg tgccctcgat tttcattatc
     1621 gtatgtgaaa tagacttgaa gacgtgcgga gtctgtcaca cgttctcgcc gcacgcaagg
     1681 cgcgcgcgcc agtcggctca actcttggcg ttgggctgga atccggccag ttctaatccc
     1741 ttggacactt ctggattttt cgcgaattgt tcccaaacga agctgcttcg cgcgttttcc
     1801 gccatgatga tggtgatccc cgcatcgatg cccaatgcgt ccgggctgct ccagttgttc
     1861 agcggattaa acgcatccac gtagccatac ttcttccata cccgcttgcc gaatttctcc
     1921 cggatattct gcagcacgct cacgcactct ttgggcagga ataccagtga gcctgccgag
     1981 gcacacggca caatgcttcc gtcaattcgt cccatcgcgg gcggtccgcc ccagatcgca
     2041 tagccgtgct cggaatccga agcgctgatt ccccacagat tctccgaata gtcggaaaac
     2101 cgctccgcca gttccaggca ccacaatttg tggattttcg acgccacaat tgaattcgca
     2161 aagtagtcgc cgtatttgtc atggatccct cgaaagttga accacgcgtg cgaatactgg
     2221 tgcgcaaata tgggcgcgtg cgctccgatg taggcaatct ggttgaattc gaattgcggc
     2281 cgttgccagc tgttccaggc cgtcggcggc agcgggtgcg tacgtgacgc catccccagc
     2341 aggtacatca tcatcagttc actgtagctg tcccaccgcg cgcgcaaaaa tccctgctcc
     2401 ggcatccagc ccatcgccag cgtctgccca ctgtgcaaca tccagttcca gtccgcccgt
     2461 tcgtagagct ccgtcgccag ctgctgaatt tcgttgtcgt caaagaagcc cttgcaggtc
     2521 agctcaccgc atagaaaaag acaagtatcc acgggcgaaa cttcggaatt cagaatccgc
     2581 tgtcccgtct ccccattcac gaagtgatag agaaacccgt gttggtgttg tacgctcttc
     2641 aacgcaaagc ccagcgtcgc ctttacgcgg tcccggatcg ggccttcttc ttcgtagccc
     2701 cgattcgcgg caatgcaaag cgccgtcagc ccgaa
//
LOCUS       DASTPM010000045         5427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1299262, whole genome shotgun sequence.
ACCESSION   DASTPM010000045 DASTPM010000000
VERSION     DASTPM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299262"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /gene="rplC"
                     /locus_tag="VFR42_00775"
     CDS             complement(<1..551)
                     /gene="rplC"
                     /locus_tag="VFR42_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU0367712.1"
                     /translation="MAVDGIIGIKLGMTQIFAEDGNLVGCTVLQAGPCVVVQRRTKEK
                     DGYDAAQLGLVEFVKPQRVNKAMTGHFKKADVAPMKVLKEVRIAESKDETKVGDRVLV
                     ENFKAGERVDVSGVSKGKGFAGGVKRWHYAGGDATHGSMFHRAPGGIGGSSFPSRVWK
                     NQHFPGHMGNQKVTAKNLKVIKV"
     gene            complement(626..955)
                     /gene="rpsJ"
                     /locus_tag="VFR42_00780"
     CDS             complement(626..955)
                     /gene="rpsJ"
                     /locus_tag="VFR42_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU0367713.1"
                     /translation="MRQGDKIRIRLKAYDHRILDQSTREIVETAKRTGADVAGPIPLP
                     TTINRWTVLRSPHVDKKSREQFEMRTHKRLIDILEPTPDTVDALMKLDLPPGVDVEIK
                     AFGHAAK"
     gene            complement(993..2180)
                     /gene="tuf"
                     /locus_tag="VFR42_00785"
     CDS             complement(993..2180)
                     /gene="tuf"
                     /locus_tag="VFR42_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU0367714.1"
                     /translation="MAKEKFERSKPHVNIGTIGHIDHGKTTLTAAITKVLSKHNPKVQ
                     FRAFDSIDNAPEERERGITIAVSHVEYETPNRHYAHMDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPAIVVFLNKCDMVEDPELLELVELEVRELLK
                     SYQFPGDTIAIVRGSALQALNGDAKWEGKIDELMEAVDKNVPLPVRDVDKPFAMPIED
                     IFSISGRGTVVTGRIERGKVKVGEEIEIVGFRPTQKKIVTGVEMFRKLLDEGIAGDNV
                     GLLLRGTEKEDVERGQVVCKPASITPHTKFKAEAYVLTKEEGGRHTPFFTGYRPQFYF
                     RTTDVTGDVKLKEGVEMVMPGDNVSCEVSLITPVALEKGLRFAIREGGHTVGAGAVTE
                     IIE"
     gene            complement(2250..2324)
                     /locus_tag="VFR42_00790"
     tRNA            complement(2250..2324)
                     /locus_tag="VFR42_00790"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2290..2292),aa:Gly,seq:tcc)
     gene            complement(2364..2439)
                     /locus_tag="VFR42_00795"
     tRNA            complement(2364..2439)
                     /locus_tag="VFR42_00795"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2404..2406),aa:Thr,seq:ggt)
     gene            complement(2604..2692)
                     /locus_tag="VFR42_00800"
     tRNA            complement(2604..2692)
                     /locus_tag="VFR42_00800"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2656..2658),aa:Tyr,seq:gta)
     gene            complement(2858..2933)
                     /locus_tag="VFR42_00805"
     tRNA            complement(2858..2933)
                     /locus_tag="VFR42_00805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2898..2900),aa:Thr,seq:tgt)
     gene            3242..4327
                     /locus_tag="VFR42_00810"
     CDS             3242..4327
                     /locus_tag="VFR42_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367715.1"
                     /translation="MRPANAKKIFRALGVAAVIATALLFVYFSSNHRDSTRAELLGLV
                     PPYTTSVVLVDLDQFRNSPFLATLYSWAPHAAEDTEYRQFIADTGFNYERDLAQVFLA
                     VSHHGGALHTLVLAEGKFDRNKIEAYLSRNSAPTQQGNLKVFRIPAWMRDKSIFFSFL
                     SDRRIAIADSENLAALLSAAKNESAHAEWQNRFDRLAGSPLFIVVRQDPTVHTVLASQ
                     SPQLATLIDQLPWITLAGKPSGNLLQVVIEGESSSDAISSQLLDFLQGIQLLAQNGLN
                     DPQLRQRMNPEERNAYIELLKGTLIDKISRGESKSVRIVLPITPQFLQVAAASKIGVM
                     PDTTTDPPPASPKRENAPQQAKPAKRN"
     gene            complement(4357..5241)
                     /locus_tag="VFR42_00815"
     CDS             complement(4357..5241)
                     /locus_tag="VFR42_00815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0367716.1"
                     /translation="MIKGKNYTFFIASTPGKLRKLVVPAYALHGLAVLALIGMITVTA
                     AVGSYSRMLWKVGNYNALRHEQESLKVQYHQLQTTVNDTNQRLDSLQSLATEVAMTYG
                     VLRYHPAAFDVSDNPVTAQDAFDRSVEQYTLLKKNAAAIAISSGGLRLMPSFSFADST
                     YTPAIWPVLGHITDSFGERLDPFSGEGAFHTGVDVASDYGAPVHVTADGVVISAENHA
                     GYGRVVVVDHGFGITTWYAHLSSFATIAGTRVKRGQVVGYCGISGRSTGPHVHYEVRM
                     NNAPVNPWRYMKSTPAGD"
BASE COUNT         1134 a         1795 c         1289 g         1209 t
ORIGIN      
        1 tccaccttga tcactttcaa attcttcgcg gtcactttct ggttgcccat gtgacccgga
       61 aagtgctggt tcttccacac ccgcgacgga aacgaactgc cgccgatgcc gcccggtgcg
      121 cggtggaaca tcgaaccgtg cgtagcatcg ccgcccgcgt agtgccagcg ctttacgccg
      181 ccggcaaaac ctttgccttt gctcacgccg ctgacgtcca cccgctcgcc ggctttgaaa
      241 ttctcgacca gcacgcgatc cccgactttc gtttcgtcct tggattccgc aatccgcact
      301 tccttcagga ccttcatcgg agcgacatcg gccttcttga agtggccggt catcgcctta
      361 ttcacgcgct gaggcttgac gaattccacc agtcccagct gcgcggcgtc atagccgtct
      421 ttttctttgg tacgccgctg caccaccacg catggtccgg cctggagcac tgtgcagccg
      481 accaaattgc cgtcttcggc aaaaatctgc gtcatgccca gcttgattcc gataatcccg
      541 tcgaccgcca tgtcctcttc tctctttcgc agcttgcctc aacttcccct gggtattggc
      601 tgcgtcctgc tggattttta aagacctact tcgcggcgtg gccaaacgct ttgatttcca
      661 cgtccacgcc cggcggcaga tcgagcttca tcagagcgtc cactgtatcc ggcgtcggct
      721 ccagaatgtc gatcaaacgc ttgtgcgtgc gcatctcaaa ctgctcgcgc gatttcttgt
      781 ccacgtgcgg cgagcgaagc accgtccagc ggttgatggt ggtcggcaga ggaatcgggc
      841 cggctacgtc cgcgccggta cgctttgccg tttccacaat ttcccgtgtg gactgatcga
      901 gaatgcggtg atcgtacgcc ttcaaacgaa tccgaatttt gtcgccttgt ctcatttttc
      961 tcttcttcag aatctctgtt tagaactacc gactactcaa taatctccgt gaccgctccc
     1021 gcgccgaccg tgtggccgcc ttcgcgaatg gcgaaccgca aacccttctc cagcgctacc
     1081 ggcgtgatca aactcacttc gcagctcacg ttgtcccccg gcatcaccat ttccacgcct
     1141 tctttcagct tcacgtcgcc ggtcacgtcc gtcgtccgga agtagaactg cggccggtac
     1201 cccgtgaaaa acggcgtgtg ccgcccgcct tcttccttcg tcaatacgta cgcttccgcc
     1261 ttgaacttcg tgtgtggcgt gatcgacgcc ggcttgcaca ccacctgccc gcgctccaca
     1321 tcttcttttt ccgtgccgcg cagcagcaat cccacgttgt cgcctgcaat cccttcatcc
     1381 agcagctttc tgaacatttc cacgcccgtc acaatcttct tctgcgtcgg acggaaaccc
     1441 acaatctcga tttcctcccc caccttcacc ttgccgcgtt cgattctgcc cgtcaccacc
     1501 gtgccgcggc cagaaatcga aaaaatatct tcaatcggca tggcgaacgg cttatccaca
     1561 tcgcgcaccg gcagcggaac gttcttgtcc accgcttcca tcaattcatc aatcttgcct
     1621 tcccacttgg catcgccgtt caacgcctgc agcgcgctgc cacgcacgat cgcaatggtg
     1681 tcgccaggaa actggtagct cttcaacaac tcccgtactt ccagttccac cagttccaaa
     1741 agctccggat cctccaccat gtcgcacttg ttcaggaaaa ccacgatcgc cggaacccca
     1801 acttgccgcg ccaacagaat atgttcccgc gtctgcggca tcggaccatc cgtcgccgcc
     1861 accaccagaa tcgcgccatc catctgtgcc gcgccggtaa tcatgttctt gatgtagtcc
     1921 gcatgtcccg ggcagtccat gtgcgcgtag tgccggttcg gcgtttcgta ctccacgtgc
     1981 gaaaccgcaa tggtgatgcc gcgctcgcgc tcctccggcg cattgtcaat cgaatcaaag
     2041 gcgcgaaact gcaccttggg attgtgctta ctcaacacct tggtgatcgc cgccgtcaat
     2101 gtcgtcttgc catggtcaat gtgaccaatc gtccctatgt tcacgtgcgg cttgctgcgc
     2161 tcgaatttct ccttggccat ctcgttaccc tcgtaagatc tgactgcaat ccggctcgca
     2221 cattagccag cacaagcctt gaaaactttt ggagcggggg acgggatttg aacccgcaac
     2281 catcaccttg gaagagtgag actctaccgt tgagttaccc ccgctcggcc cttcccaaac
     2341 cccaattccc aaacctttaa aactggagcc tgggaccggg attgaaccgg tgacctcgtc
     2401 cttaccaagg acgcgctcta ccaactgagc tacccaggct aagtccgtct tgctacccag
     2461 ccccagacaa cttcgcgacg gcgcacttca ccttaccggc taggtcgccg aattttagtc
     2521 cgcctgtgaa caactctggt ggttgccctg cggtttactg cgaactggct ttcatctcgc
     2581 gccgtgcgcg ttttccgaaa gcctggtgga cggggaagga ttcgaacctt cgtagcccgc
     2641 aaggagcggc agatttacag tctgctggtt ttagccactc acccacccgt cccaaaaatc
     2701 gggacgcaga attcgtgtcc caaaatcgtt tcgcttcaat aggttcggca cagttcagcg
     2761 gaaacacaaa acggactcgt gtccaaagac accagcccgt tcaattttga ctactagaat
     2821 tgctcccgcc tcactcactc ccctgccgaa ctcatggtgg agctggcgga gggaattgaa
     2881 cccccgaccc tctgattaca aatcagatgc tctaccagct gagctacgcc agcgtcgctc
     2941 aaaccgcgta agaatagcag actcggaatc cgaattgcaa ggggccggct caaaccgcca
     3001 gcagttagac agccagccct gtggaaatcc cgcatattgt ttcctgattc aatcgcaagt
     3061 aaccgactgt aaattcagca ggttagaaga ggacgcgtcc ctttaactgc agactcgaca
     3121 gtgccagcga gaatcgaccg attttttatt ttcctgctcc ccacaacaga ccctgcaagc
     3181 caggacttta agcaatacgg taagatgctc gttccgcaaa ggagttcgca cgataccaaa
     3241 catgcggccc gcgaacgcga agaaaatttt ccgcgcactc ggagtagcgg ctgtaatcgc
     3301 tacggcgctg ctttttgttt acttctcaag taaccatcgc gattctaccc gcgccgaact
     3361 tcttggcttg gtgcccccct acacaacgtc cgtcgttctg gtcgatctcg atcaattccg
     3421 caattccccc ttcctcgcca cactctactc gtgggccccc cacgccgccg aagacacgga
     3481 atataggcag ttcattgccg acaccggctt taattacgaa cgcgatctgg cgcaagtctt
     3541 cctcgccgtc tcccatcatg gaggcgcttt gcataccctt gtactagccg aaggaaaatt
     3601 cgatcgcaat aaaatcgaag cctatctcag tcgcaactcg gcccccacgc aacaaggcaa
     3661 tctcaaggtt tttcgcatcc ccgcatggat gcgcgacaaa tccattttct tttcgtttct
     3721 ttctgaccga cgcattgcca tcgccgactc ggaaaatctc gcagcgctgc tttccgctgc
     3781 gaagaacgaa tcggctcacg ccgaatggca gaatcgcttt gatcgtcttg ctggttcgcc
     3841 actcttcatc gtcgttcgcc aggatccaac cgtacatacg gtgttggcta gccagtctcc
     3901 gcaactcgca acactcatcg atcagcttcc ctggatcacc cttgccggca aacccagcgg
     3961 taatctgctg caagtagtca tcgaaggaga aagttcctcc gacgctataa gttctcagct
     4021 tctcgacttc ctgcagggca tccaactgct cgcccagaac ggcctgaacg atccccaact
     4081 ccgccagcgg atgaatcccg aagaacgcaa cgcctacatc gagctcttga agggaacgct
     4141 gatcgacaag atcagccgcg gcgaatcgaa atccgtccgt atcgtgcttc ccatcacgcc
     4201 gcagttcctg caagttgccg ccgcgtccaa gatcggagtc atgcccgata ctacaacgga
     4261 cccgccccca gccagcccga agcgggaaaa tgcgccgcag caggccaagc ccgcaaagag
     4321 aaactagccg gtcagtaatt ttaaggaagc tcgtgctcag tcgccggcag gagtggactt
     4381 catgtaccgc caagggttga ccggggcgtt gttcatccgc acttcgtaat gcacgtgcgg
     4441 cccagtcgat cgcccgctga tgccacaata tccaaccacc tggccacgct tcacgcgtgt
     4501 acccgcaatc gtcgcaaatg aagaaaggtg cgcgtaccag gtcgtaattc caaatccgtg
     4561 atcgaccacc accacgcggc cgtagcccgc atgattctcg gcgctgatca ccacgccgtc
     4621 cgccgtcacg tgtaccggag cgccataatc cgaagccaca tccacgcccg tgtggaaggc
     4681 gccttcaccg ctgaacggat ccagccgttc gccaaagcta tccgtgatgt gtcccagcac
     4741 cggccaaatc gccggcgtgt aggtcgaatc cgcaaagctg aacgacggca tcagccgcag
     4801 cccacccgac gaaatcgcaa tcgccgccgc atttttcttc aagagggtgt actgctcgac
     4861 ggaacgatcg aaggcatcct gggccgtcac cgggttatcc gaaacatcga acgccgccgg
     4921 atgataccgc agcactccgt acgtcatcgc cacttctgtc gccagggact gcagcgagtc
     4981 gagccgctgg ttggtatcgt taacggttgt ctgcagttgg tggtattgca ctttcaggga
     5041 ttcctgttcg tggcgcagcg cgttgtagtt gccgaccttc cacaacatcc gcgagtagga
     5101 cccaaccgcc gccgtaaccg tgatcatccc gatcagcgca agcaccgcca gtccgtgcag
     5161 cgcataagcc ggtaccacca gcttgcgcag cttgccgggc gtcgaggcaa taaagaatgt
     5221 atagttcttg cctttgatca caaacggccc ctcgggcctt ttctttccct acttcgaacg
     5281 cgggcaaccg aactgttcca cccgcaaaga accgcaagaa cgaacgttgg ttcgaattcg
     5341 aagcatcatc agcaagcctc gaattgccat cgtttaaatc tgaaccgtaa gttatggtcc
     5401 gcgaccgctt ctatcctagt tttgtag
//
LOCUS       DASTPM010000046         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_434296, whole genome shotgun sequence.
ACCESSION   DASTPM010000046 DASTPM010000000
VERSION     DASTPM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434296"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1404
                     /gene="pqqE"
                     /locus_tag="VFR42_00820"
     CDS             280..1404
                     /gene="pqqE"
                     /locus_tag="VFR42_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEU0367717.1"
                     /translation="MNAPLALIAELTHRCPLHCVYCSNPRELAARSEELPTEVWARVF
                     AEAAAAGVMQLDLTGGEPLARPDLTKLIQSARAAGLYVNLITSGLPLDEIRLDELVAA
                     GLDHFQLSFQGAREETANEISGTKTHAQKLKVLGWLKSRRVAVTLNFVLHRNNLDQLN
                     EMLSVAENSSASRIEFAHVQYYGWAFANRDQLLPSREQLMQSLEFFKQAEERLRGKIK
                     VEYVVPDYYAKFPKPCMGGWGRKAMLITPRGDAMPCHAASVIPGLRFDNVKDKSVKEF
                     WESSEAFQKFRGETWMQEPCKSCDRRTQDFGGCRCQAMLLAGNAAATDPVCSLSPERL
                     RVDSILAAIPAQREHSAPRVASQPTPALLLPQWLYRPNPQ"
     gene            complement(1469..>2073)
                     /locus_tag="VFR42_00825"
     CDS             complement(1469..>2073)
                     /locus_tag="VFR42_00825"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0367718.1"
                     /translation="FAMNDRPIRSTLLLMSMLSIFGLQYSVFMPIFAEDVLHSGARGL
                     GFLMSAAGVGAVLGALQFAARTAYRGLAKWIAAMSVICSVCLLIFSQSKVFWLSILVL
                     FIVGFAATSQMAATNTTVQNRSPEQLRGRLMAVYATMFMGVQPIGALLAGGLAKRFGA
                     PMTLAIFGSVCLLGSLLFAAWVVLRVRPAEQAATSAVGGK"
BASE COUNT          452 a          730 c          496 g          395 t
ORIGIN      
        1 ttctcgcccc tcgctccgcg gcagtttttg attccccggc gttgctaggt cccttccatt
       61 cctcacttac agacgaactc cgcgtttcac ccgaaattag ccgaaatcgc cgcagactaa
      121 gtcctttggt tgccacactt acgcaaactc gcacccgcaa gcttgtttgc cgtactcaca
      181 aaaaaatcgg ggagggggta cccgttccga atcgggaatt ttgtagattg cattgtggga
      241 ggtttgcctc gcttaggcgg accgctataa cttagaccta tgaacgctcc gctcgccctt
      301 atcgccgaac tcacccaccg ctgtccgctc cactgcgtct actgctccaa cccacgcgaa
      361 ctcgccgctc ggagcgaaga gcttcccacc gaggtatggg cgcgcgtttt tgccgaggcc
      421 gctgccgccg gcgtcatgca gctcgacctc accggcggcg aacctctcgc ccgccccgac
      481 ctcactaaac tcatccagtc cgcgcgggcc gctggactct atgtcaacct catcacttcc
      541 ggccttccgc tcgatgaaat acgcctggac gaactcgtcg ccgccggcct cgaccacttc
      601 cagctcagtt ttcaaggcgc tcgcgaagaa accgccaacg aaatctctgg caccaaaacc
      661 cacgcgcaaa aactcaaagt gctcggctgg ctcaagtccc gccgcgtcgc cgtcaccctc
      721 aattttgttc tgcatcgcaa caaccttgat caactcaacg agatgctttc cgtcgcggaa
      781 aattcttccg cctcccgcat cgaattcgcc cacgtgcaat attacggctg ggccttcgcc
      841 aaccgtgacc aactccttcc ctctcgcgaa cagctcatgc agtcactgga atttttcaag
      901 caggccgaag aacgcctgcg cggcaaaatc aaagtcgaat acgtcgttcc cgattactac
      961 gcgaaatttc ccaagccctg catgggcggt tggggccgca aggccatgct cattacgcca
     1021 cgcggcgatg caatgccgtg ccatgccgcg agtgtcattc ccggcctgcg cttcgataac
     1081 gtgaaagaca aatccgtaaa agaattttgg gaatcgagcg aagcgtttca gaaattccgc
     1141 ggcgaaacgt ggatgcagga accgtgtaaa tcctgcgacc gccgcaccca ggacttcggc
     1201 ggctgccgct gccaggccat gctgcttgcc ggaaacgccg ccgctaccga ccctgtttgc
     1261 tctctctctc cggagcgcct ccgcgtggac tccatcctcg ctgcaatccc cgcgcagcga
     1321 gagcactccg ctccgcgggt ggcctcgcaa ccaacccctg ccctgttgct accccaatgg
     1381 ctctaccggc cgaacccaca atgaatggaa atgagaaaat tgaaaatggc gccctcagag
     1441 cgggttcctc ttttccatct tcccttttct attttccgcc gacggcgctt gtcgccgcct
     1501 gctccgctgg ccgcacacgc agcaccaccc acgcggcgaa cagcaggctt cccagcaaac
     1561 aaacgctgcc aaagatcgcc agcgtcatcg gcgcaccgaa ccgcttggca agccctccag
     1621 ccagaagcgc accgatcggt tgtacgccca tgaacatcgt cgcgtacacc gccatcagcc
     1681 gcccgcgcaa ctgttccggc gaacgattct gcacggtggt gtttgtagcg gccatctgcg
     1741 aagtggccgc aaaccccaca atgaacagca ccaggatgga cagccagaag actttcgact
     1801 gcgaaaaaat cagcaggcat accgaacaga tcaccgacat cgccgcgatc catttcgcca
     1861 atcccctgta ggccgtgcgc gccgcaaact gcagcgctcc cagcactgcg ccgacccccg
     1921 ccgcgctcat caagaatccc aatccgcgcg cgccggaatg caaaacatct tcagcgaaaa
     1981 tcggcatgaa caccgaatac tgcaaaccaa aaatgctgag catgctcatc agcaacaacg
     2041 tcgaccgtat cggccggtcg ttcatcgcga agc
//
LOCUS       DASTPM010000047         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1212607, whole genome shotgun sequence.
ACCESSION   DASTPM010000047 DASTPM010000000
VERSION     DASTPM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212607"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VFR42_00830"
     CDS             <1..1368
                     /locus_tag="VFR42_00830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0367719.1"
                     /translation="GGMIPPGSRVLELGCGTGDLLASLKPRRAIGLNFAEGLTERARQ
                     KYPEFEFHTTGVDSAAFPRAFEPDYVVMTNMLDYVYDVWDVVDTLKPGISEHTLLIVT
                     TNNPLWAPLLRLASRLGLRVPESARNFITNRDICSVLSLQGFSIVEEGLLLPVPKRVP
                     LLGSLLNAVIPELPAIRFLSSIQYIAARRRVLRPKLSCSVVIPCHNEAENIAECVRRV
                     PQLGDWTELVVVDDGSTDGTRDKVAELMREDPRVRLIVLEKNQGKASAVRAGFSAARG
                     DVLMILDADMAVNPEELSKFLIPLQEGTADFVNGTRLIYPMHGKAMKTANFLGNKGFC
                     FLASKALRQRVSDTLCGTKAFFRRDYLRMPIGGKERWGDFDFLFGAARLKLRILELPV
                     HYTERRAGKSKMRVVVDGWRFLRACVTGWRMLRFPRTHPWGQRLPAVEGSHELRVDAE
                     VPAIR"
     gene            1374..2168
                     /locus_tag="VFR42_00835"
     CDS             1374..2168
                     /locus_tag="VFR42_00835"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0367720.1"
                     /translation="MPIDTQAREAFVRHRRVWQEKPVLRRIYNEEFFAKLLSSRVPGG
                     ISIEVGGGPGFFKELLPAVISTDLIPSPALDAVVDAQILPFRSASVTNIFGLDILHHI
                     AAPMAFLQEAQRVLAVGGRLILVEPWITPFSRFVYRYLHQEDCDLSARPWELNGSRGP
                     GKKAFEGNQAIPYLLFGPRAIARTLKTLPNLKLIKLETFCLFAYLLSFGFKPMNLMPE
                     FLYPALAGFERRTLPLWRRVAALRVLLVLEKISDSGRLAGSSASQG"
     gene            2176..>2518
                     /locus_tag="VFR42_00840"
     CDS             2176..>2518
                     /locus_tag="VFR42_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367721.1"
                     /translation="MRDKSSISLFTSAYNLARKARLLELPWFRRAFVSSYFLYKQHYE
                     DPFMELTRRMPGIFHNGDILDIGANIGYTAWVFAGALRPGARVYAFEPDDSSFALLQE
                     IIQRRKLSASVE"
BASE COUNT          440 a          796 c          721 g          561 t
ORIGIN      
        1 ggcggaatga ttccccccgg ttcgcgcgtt ctggagctcg gctgcggcac cggagacctg
       61 ctggcctcgc tgaaaccccg gcgcgccata ggactcaatt ttgccgaagg cctgaccgaa
      121 cgggcgcgcc agaaatatcc ggaattcgag ttccacacca ccggggttga ttcggcagct
      181 ttcccgcgtg ctttcgaacc cgactacgtg gtcatgacca acatgctcga ctacgtctac
      241 gacgtctggg atgtggtgga caccctgaag ccgggcatct ccgaacatac tttgctcatc
      301 gtcaccacca ataacccgct gtgggctccg ctgctgcgct tggccagccg gctgggcctg
      361 cgcgtgccgg aatcggcgcg caactttatc accaaccgcg acatctgcag cgtgctcagc
      421 ctgcaaggat tcagcatcgt cgaagaaggc ttgctgctcc cggtgcccaa gcgcgtgccg
      481 ttgctcggct cgctgctgaa cgccgttatc ccggagttgc cggccattcg tttcctttcc
      541 tccattcaat acattgccgc tcgccgccgc gtgctgcggc cgaagctctc ctgctcggtg
      601 gtgatccctt gccacaacga ggccgaaaac atcgcggaat gcgtgcggcg cgtcccgcag
      661 ctgggcgatt ggacggaact cgtggtcgtc gatgatggct ccacggatgg cacccgcgac
      721 aaagttgcgg agctgatgcg cgaagatccc cgggtgcgcc tgattgtcct ggagaagaat
      781 caaggcaaag ccagcgcggt gcgcgccggt ttttccgccg cgcgtggcga tgtgctgatg
      841 attctggacg ccgatatggc cgtcaacccc gaggagctct ccaaattttt gatccccttg
      901 caggaaggca ccgccgactt cgtgaatggc acccggctga tttacccgat gcatggcaaa
      961 gccatgaaaa ccgctaattt ccttggcaac aagggctttt gcttcctggc cagcaaagcg
     1021 ctccgccagc gggtcagcga cacattgtgc ggcaccaagg catttttccg ccgcgactac
     1081 ctgcgcatgc ccattggcgg aaaagagcgc tggggcgatt tcgatttcct gttcggcgcc
     1141 gcgcgcctga aactgcgaat cctcgagcta cccgtgcact acaccgagcg tcgcgccgga
     1201 aaatccaaga tgcgcgtggt cgtggatggc tggcgcttct tgcgagcctg tgtcaccgga
     1261 tggcgcatgt tgcgctttcc gcggacccat ccctggggcc agcgtctgcc cgccgttgag
     1321 ggcagccacg agcttcgcgt tgacgccgaa gtccccgcaa ttcgttagct accgtgccca
     1381 tcgacactca agcccgcgaa gcgttcgtcc gccatcgccg cgtctggcag gaaaagcccg
     1441 tcctgcggcg gatctacaac gaagagtttt ttgccaagct gctttcttcc cgggtgcccg
     1501 ggggaatctc catcgaggtt ggcggcggac ccggattctt caaggaactg ctgccagcag
     1561 tgatttccac cgacctgatt ccaagccctg cgcttgacgc cgtggtggac gcgcagattt
     1621 tgccctttcg ctccgccagc gtaaccaaca ttttcggctt ggacatcctg caccatatcg
     1681 ccgcgcccat ggcctttctt caggaagcgc aacgcgttct ggccgtgggt ggccgcctga
     1741 tcctggtcga accctggatt actccctttt cgcgcttcgt gtaccgctac ctgcatcagg
     1801 aggattgcga tctttccgcg cgcccctggg agctgaacgg cagtcgcgga cccggcaaga
     1861 aggcgtttga aggcaatcag gccattccgt atttgctctt cggtccgcgc gccatcgccc
     1921 ggacactgaa aacgcttccg aatctgaaac tcattaagct ggaaacgttt tgtttgtttg
     1981 cgtatttatt gagctttggt ttcaagccca tgaatctcat gcccgaattt ctttatccgg
     2041 ccctggcggg attcgagcgg cgtacgctgc cgctctggcg tcgcgtggcg gccttgcgcg
     2101 tcctgctggt cctcgagaaa atttccgatt ctggccggct cgccggctct tcagcatccc
     2161 aggggtgatt ggcgtatgcg cgacaaaagt tcaatttcgc tgttcacctc cgcctataat
     2221 ctggcgcgca aagcgcgctt gctggagttg ccttggttcc gccgcgcctt tgtgtcctcc
     2281 tattttcttt acaagcagca ttacgaagat ccgttcatgg aattgacgcg acgcatgccc
     2341 ggaatttttc acaacggcga catccttgat atcggcgcaa acattggcta taccgcctgg
     2401 gtattcgccg gcgcgctccg gccgggggcc cgggtctatg cctttgaacc ggatgattcc
     2461 agttttgcgc tgctccagga aattattcag cgccggaagc tatccgcctc ggtggaag
//
LOCUS       DASTPM010000048         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_751447, whole genome shotgun sequence.
ACCESSION   DASTPM010000048 DASTPM010000000
VERSION     DASTPM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751447"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1531
                     /locus_tag="VFR42_00845"
     CDS             185..1531
                     /locus_tag="VFR42_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367722.1"
                     /translation="MKLFRQGIGVRALALTVFALGLTTLGNRGTTAAQVSIGISVNFA
                     PPELPVYEQPLCPGEGYLWTPGYWAWDSDYGDYYWVPGTWVLAPQPGYLWTPPYWGWS
                     GAAFVFHEGYWGPRVGFYGGINYGYGYTGRGYYGGRWERERFYYNRNVNNINVTQIHN
                     VYNQTVVNNINVTRVSYNGGNGGIRERANRQEEQAARERHMGAVALQQQHIQEARTDR
                     ALRASDNHGKPPIAATDRPGQFRGGNVVPAKAGNYNPPPNRGGGGNGGRPDNNRVENN
                     RPDNRVDNHPNNRPNERPDSRVDNRPERNDRPPNARGPESNSSRPTYVHPNEAPKANR
                     PDRPPNSGNAKADQKYQQRTDNMYRKQEQERQNLQKQQDQQHARAEQQKANDARKQQM
                     EKQHQQQTQKMEQRQQQQQQKMERQAPKPKPQAQPKQQPPQQQQPKQQGGQHEKQP"
     gene            1633..1860
                     /locus_tag="VFR42_00850"
     CDS             1633..1860
                     /locus_tag="VFR42_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367723.1"
                     /translation="MANSVSKIQIGQLWKKDGTGETFLVTRVYSEALSTMATLRKSGA
                     ESEALVRVKVERAAGGQTLPGFSPAQEDERI"
     gene            complement(1972..2688)
                     /gene="bshB1"
                     /locus_tag="VFR42_00855"
     CDS             complement(1972..2688)
                     /gene="bshB1"
                     /locus_tag="VFR42_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEU0367724.1"
                     /translation="MTLDILAIAAHPDDVELTCGGTLLKSAQQGYKTGILDLTAGEMG
                     TRGTPGTRAKEAARAAKILKVSYRGTLGVPDSDVQPSRQHKLALAQRIRELRPHTVIL
                     PYWEARHPDHYHASALGYEGCFLAGLKALPIAGKPFRPFKILYCTSFEGMPATFVVDI
                     TKQYATRRRAILAYASQFRPAASERKQRVYLAIDELDSKMDLLARRNGDLIGVEYGEP
                     FLQKEPMQIEDVVNLPVRSI"
     gene            2789..>3240
                     /locus_tag="VFR42_00860"
     CDS             2789..>3240
                     /locus_tag="VFR42_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0367725.1"
                     /translation="MKQKLEGLEGKVALITGAGKRLGRAVALRLAEEGMDVAVHYGTS
                     AKDAAEVVGKIEEMGKKAVAIRADLRRVDEIRRMLLEVGNELGRLDVLVNSAANFLPG
                     SIISTTEEVWDASLDTNLKAPFFLAQAAAPMLRRSKGAIVNFADTGGVL"
BASE COUNT          774 a          774 c         1158 g          534 t
ORIGIN      
        1 ccgacgagga tgccacgcgc ggagttctac atgggattgg gtataggtga aaccaaaacg
       61 cgcggctagg gtttaataag tgagcgaagc ggattagcta gggaacactt cgccggagac
      121 aggtcgcgcc cgcagccagg aaacgagaac caatccagaa agaccagtgc gggcaggagg
      181 aagcatgaag ctttttcgac aaggaatagg tgtgcgggcc ctggcactga cagtgttcgc
      241 gttgggtttg acgacgttgg gaaaccgagg cacaacggca gcgcaagttt cgatcgggat
      301 ttcggtaaat tttgcgccgc cggaactacc ggtgtacgag cagccattgt gcccgggaga
      361 agggtacctc tggacgcccg gatactgggc gtgggattcc gactatggcg attactactg
      421 ggtgcccgga acttgggtgc tggcgccgca accgggatac ttgtggacgc cgccgtactg
      481 gggatggagc ggcgcagcgt tcgtttttca tgaaggctac tgggggccgc gcgtcggttt
      541 ctacggcgga atcaactatg ggtatggcta caccggacgc ggatattatg gcgggcggtg
      601 ggaacgggag cgcttttact acaaccgaaa cgtgaacaac atcaatgtca cgcagattca
      661 caacgtctat aaccagacgg tggtgaacaa catcaacgtg acccgggtga gctacaacgg
      721 cggaaacggc ggaatccgcg aacgtgcaaa ccggcaggaa gaacaagcag cacgggaacg
      781 gcacatgggc gctgtggcgc tgcaacagca gcatattcaa gaggcgcgaa cggatcgcgc
      841 gctgagagcg tcggataatc acggcaagcc gccaattgcg gcgaccgacc ggccggggca
      901 gttccgcggg ggcaatgtgg tgcccgccaa agcagggaac tacaacccac cgccgaaccg
      961 cggaggaggg ggcaacggcg gacgcccgga caataatcgc gtggaaaaca atcggccgga
     1021 caatcgcgta gacaaccacc caaataaccg gcccaatgag cgcccggata gtcgcgtcga
     1081 caaccggcca gagcgcaacg atcggccgcc gaacgcgaga gggccggaaa gcaacagcag
     1141 ccggccgacg tatgtgcatc ccaatgaagc gccgaaggcg aacaggccgg atcgtccccc
     1201 gaacagcggg aacgcgaagg cggaccagaa atatcagcag cgcaccgaca atatgtacag
     1261 gaagcaggaa caggaacgcc agaacctgca gaagcagcag gaccagcagc atgcgcgagc
     1321 ggaacagcaa aaagcgaacg atgcgcgcaa gcagcagatg gagaagcagc accagcaaca
     1381 aacccagaag atggaacagc gtcagcagca acaacagcag aagatggagc ggcaggcacc
     1441 caaaccgaag ccgcaagctc agccgaaaca acagccgccc cagcaacagc agcctaagca
     1501 gcagggcgga caacacgaaa aacagccgta gaggtctgct taaagcgcag acggaaaacc
     1561 ggcttgcttc gcaggatgca tgggcgaggc gagccggttg acacgttggg gctgtgacca
     1621 tacagtcagg ccatggcgaa tagcgtgtcg aaaattcaga ttgggcaatt gtggaagaag
     1681 gatggaacgg gcgagacgtt cctggtgacg cgagtgtact cggaagcctt gagcacgatg
     1741 gcgacgctgc ggaagtcggg cgcggagagt gaagcgctgg tgagggtgaa ggtggaacgc
     1801 gctgcgggcg ggcagacgct gccggggttt tcaccggcgc aagaagatga gcggatttga
     1861 gtgggaagta gacagtcgac agttgaaagt cgaaagaaaa gcaagattcg tcccttcgct
     1921 agccacggcg ggcaggatcg gaacggcagc gttggttcat ggcctctagg gtcagatcga
     1981 gcggacgggg agattaacta cgtcttcgat ttgcataggt tctttctgaa ggaacggttc
     2041 gccgtattca acaccaatca gatcgccatt gcggcgggcg agtaaatcca ttttagagtc
     2101 gagttcgtcg atggccaggt agacgcgctg tttgcgttcg ctggcggcgg gccgaaattg
     2161 cgacgcgtag gccaggatag cgcggcggcg cgtggcgtat tgtttggtga tgtccaccac
     2221 aaacgtggcg ggcatgcctt cgaacgatgt gcagtacaga attttgaagg ggcggaaggg
     2281 tttgccggcg atggggagag ccttgagtcc ggcgaggaag cagccttcgt agccaagcgc
     2341 ggatgcatgg tagtgatcgg ggtggcgagc ttcccagtaa ggaaggatga cagtgtgggg
     2401 gcggagttcg cggattcttt gagcaagcgc gagtttatgt tggcgcgaag gctggacgtc
     2461 ggagtcaggt acgccgagtg tgccgcgata gctgaccttg agaattttag cggcgcgggc
     2521 ggcttctttc gcgcgtgttc cgggagttcc gcgcgtgccc atttcgccag cagtgaggtc
     2581 gaggatgccg gttttgtagc cctgctgagc ggatttgagc agagtgccgc cgcaggtgag
     2641 ttcgacgtcg tcggggtggg cggcgatggc caggatgtcg agggtcatgg gtgctccgtt
     2701 gtggtgacgg ggaattgtaa aggtgagtgg cgacttgtga ctagtgactc tcgaagcggc
     2761 gcggcggtag atgtgttaat tttggcgcat gaaacaaaag ctggaaggcc tggaaggcaa
     2821 agtggcgctg ataacgggcg cggggaaacg gctgggacgt gcggtggcgc tgcggctggc
     2881 ggaagaggga atggatgtgg cggtgcatta cggcacgtcg gcgaaagacg cggcggaggt
     2941 tgtgggaaag atcgaggaga tggggaagaa agcggtggcg atccgcgcgg atttgcggag
     3001 ggtggacgag attcggcgca tgctgctgga agtgggaaac gagttgggac ggctggatgt
     3061 gctggtgaat agcgcagcga atttcttgcc gggaagcata atttcgacga cggaagaagt
     3121 gtgggacgct tcgctggaca cgaatctgaa ggcgccattt tttctggcgc aggcggcggc
     3181 gccgatgctg cggcgttcga aaggcgcaat cgtgaatttt gcggataccg gtggggtgct
//
LOCUS       DASTPM010000049         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_146017, whole genome shotgun sequence.
ACCESSION   DASTPM010000049 DASTPM010000000
VERSION     DASTPM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146017"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFR42_00865"
     CDS             <1..623
                     /locus_tag="VFR42_00865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU0367726.1"
                     /translation="EALLTENRIWQARLKGVGKISLDQAIAYSMSGPTLRAAGLAHDN
                     RKLFPYSSYEEFKFDVPTRTDSDCYGRYMLRVAEIRESLKIVEQAMAKITDSGPIRAE
                     APGIVPPEREKMKTEMEALIYHFKIFTEGFSPPPGDAYVAIESPRGELGCYIASDGSP
                     KPLRCHFRAPSFVNLQALPLLIQGQLIADVIACIGTIDIVLGDVDR"
     gene            639..2453
                     /gene="nuoF"
                     /locus_tag="VFR42_00870"
     CDS             639..2453
                     /gene="nuoF"
                     /locus_tag="VFR42_00870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU0367727.1"
                     /translation="MELSPQLAARFEKLQNAYPMKRSALIPMLMYAQDEAGFVSDEMI
                     AEIARRLDLRTVEVEETLAYYSMLRRKPVGKHHVQVCTNVACMLRGGYEILEQAKKRL
                     EIGHKETTNDGVFSLEEVECIGACTGAPAIQVNYDFYENLTPKQFDRIIEELDAGKRP
                     APVSVISGALHDRDPQETVLLSKRWGIKDSRKIDVYLQNDGYQALEKALQQMTPESII
                     DEVKKSSLRGRGGAGFPTGMKWSFVPKDSPKARYVICNADESEPGTCKDRPLMEMDPH
                     QLIEGMIIAGRAIGAHQGFIYIRGEYRYVLDLVEEAIEEAYQRGYLGKNILGSGFDYD
                     LLIHTGAGAYECGEESALMESLEGKRGYPRIKPPFPAVVGLYGCPTIINNVETLSAVP
                     AIIREGGEAYANRGTPKNGGTRLLCVAGHVNKPGIYEIPLGMNMKKFIYEMAGGIPNG
                     KKLKAVIPGGSSCPLMAADEIDLPMDYDAVAKAGSMLGSGGMVVMDEDTCMVDMARRI
                     MHFYAHESCGWCIPCREGTIWLRKMLERFHAGLGRSEDIDLVGELAKNMLGRTFCPLG
                     DAAAMPTISIVEKFRSEFEDHLHGRCAYKSAEAVVGTR"
BASE COUNT          579 a          727 c          716 g          459 t
ORIGIN      
        1 acgaggcatt gttgaccgaa aaccgcatct ggcaagcgcg cctgaagggc gtgggaaaga
       61 tttcactcga ccaagccatc gcttacagca tgagcgggcc gacgctacgg gcggcgggcc
      121 tggcgcacga caaccgcaag ctgtttccgt attcgagcta cgaagagttc aaattcgacg
      181 tgcccacgcg taccgactcg gattgctatg gccgctacat gctgcgcgtg gcggaaattc
      241 gcgagagcct gaaaatcgtc gagcaggcca tggcgaagat taccgactcc gggccgatcc
      301 gggcggaagc gccgggcatc gttcctcctg agcgcgaaaa aatgaagacg gagatggaag
      361 cgctcatcta ccacttcaag atcttcaccg aaggcttttc gccgccgccg ggcgacgcct
      421 atgtagccat tgaatcgccg cgcggggagc tgggctgcta catcgccagc gatggctcgc
      481 caaagccgct gcgctgccat ttccgcgcgc cttcgtttgt aaatttgcag gccttaccgc
      541 ttttgattca gggccagcta atcgcggacg tgattgcgtg tatcggcacg atcgatatcg
      601 tactgggtga cgtcgaccgg taatcggcca aagggattat ggaactttcg ccacaactcg
      661 cggcacggtt tgagaagctg cagaacgcct atcccatgaa gcgcagcgcg ttgattccga
      721 tgctgatgta tgcgcaggat gaagcaggtt ttgtcagcga cgaaatgatc gcggaaatcg
      781 cgcggcgttt ggatttgcgc acggtcgaag tcgaagaaac attggcgtac tactcgatgc
      841 tccgccgcaa gcccgtgggc aaacatcacg tacaggtttg caccaatgtc gcctgcatgc
      901 tgcgcggcgg ctatgaaatt ctcgaacaag ccaagaaacg cctggaaatc ggccacaagg
      961 aaaccaccaa cgacggcgtg ttctctctcg aagaagtcga atgcattggc gcgtgcaccg
     1021 gcgcccctgc catccaggtc aattacgatt tctacgaaaa tctgacgccg aaacagttcg
     1081 atcgcatcat cgaagaactc gacgcaggga aaagacccgc accggtgagc gtgatttccg
     1141 gcgcgctgca cgatcgtgat ccccaggaaa cggtgcttct cagcaagcgc tggggcatca
     1201 aagattcccg caagatcgat gtctatctgc aaaacgacgg ctatcaggca ctggaaaaag
     1261 cgctgcagca gatgacgccg gaatcgatca tcgacgaagt caagaagtcc agcctacgcg
     1321 ggcgcggcgg tgccggcttc cccactggca tgaagtggtc ctttgtgcca aaagattcgc
     1381 cgaaggcgcg ctacgtcatc tgcaacgccg atgaatccga gcccggcacc tgcaaagacc
     1441 gtccgctgat ggaaatggac ccgcaccagt tgatcgaggg tatgatcatc gcgggccgcg
     1501 ccatcggagc ccatcaaggt ttcatataca ttcgcggcga gtatcgttac gtgcttgatc
     1561 tcgtcgaaga agccatcgag gaagcgtacc agcgcggcta tctcggcaaa aacattctcg
     1621 gcagcggctt cgactacgac ctgctgattc acaccggcgc gggagcttac gagtgcggcg
     1681 aagagtccgc actgatggaa tcgctcgaag gcaagcgcgg ctatccccgc atcaagccgc
     1741 ccttccctgc cgtagtcggc ctctacggct gcccgaccat tattaataac gtggaaaccc
     1801 tcagcgccgt tccggctatc atccgcgagg gcggcgaagc gtacgccaat cgcggcacgc
     1861 cgaaaaacgg cggcacgcgt ttgctttgtg tcgccggcca cgtcaacaaa ccgggcatct
     1921 acgaaattcc actcggcatg aacatgaaaa agttcatcta cgaaatggcc gggggcattc
     1981 ccaacggcaa aaaactgaaa gccgtcattc ccggaggcag ctcctgcccg ctgatggctg
     2041 ccgacgaaat cgatttgcca atggattacg acgcggttgc caaggccggc agcatgcttg
     2101 ggtccggcgg catggtggtg atggacgaag acacctgcat ggtcgatatg gcgcgccgca
     2161 tcatgcattt ttatgcacac gaatcttgcg gctggtgcat cccctgccgc gaaggaacca
     2221 tctggctgcg caaaatgctg gagcgtttcc acgccggcct gggccgcagc gaagacattg
     2281 atctggtcgg tgaactcgca aaaaacatgc tcggccgaac cttctgcccg ctaggcgacg
     2341 ccgccgcgat gccgaccatc agcatcgtgg agaaattccg cagcgaattc gaagaccact
     2401 tgcacggtcg ctgcgcttat aaatcagccg aagcggtggt aggcacccgg tgaaacaaca
     2461 ctgctcatga acgaacagaa g
//
LOCUS       DASTPM010000050         5392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_146071, whole genome shotgun sequence.
ACCESSION   DASTPM010000050 DASTPM010000000
VERSION     DASTPM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146071"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..485)
                     /locus_tag="VFR42_00875"
     CDS             complement(120..485)
                     /locus_tag="VFR42_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0367728.1"
                     /translation="MRFLSIYKSPEKNAPPTQEEMEKMGKLIEEGMRAGWLLGTEGCL
                     PSALGARVRLASGNFTVTDGPFTESKELVGGFAILQAKSKQDAIELVRGFLRVVGQGE
                     CELRQLYEVPAEQSHSAAS"
     gene            complement(601..1020)
                     /locus_tag="VFR42_00880"
     CDS             complement(601..1020)
                     /locus_tag="VFR42_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0367729.1"
                     /translation="MRFMMIVKANQDSEAGVMPSEEMIATMGKFNEELLKAGVLVDLS
                     GLQPSSKGFRVKFSGGKRTIVDGPFAESKELIAGYWVINVKSRQEALEWAKKVPAPGP
                     NGEGEIEVRQFFELEDFAPSPAIDQARELGKKLAAKK"
     gene            complement(1156..1593)
                     /locus_tag="VFR42_00885"
     CDS             complement(1156..1593)
                     /locus_tag="VFR42_00885"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU0367730.1"
                     /translation="MKKTTKSGRRPSAKGNSAKQHSGSGDVDKYLARVPHPARTTLQK
                     IRAVIRSAAPREATEAISYGIPAFKYNGMLVAYAAFANHCSFFPASGELLKEFAEDLK
                     DYPCSKGTIRFAVDKPLPAALVKKLVKARVAKNEAKLKAKKAR"
     gene            complement(1684..2052)
                     /locus_tag="VFR42_00890"
     CDS             complement(1684..2052)
                     /locus_tag="VFR42_00890"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0367731.1"
                     /translation="MMSLPAPVEAQENFVPTRELVTAMRKYSDDMRKAGVLLLAEGLH
                     PSSKATRIKMSGGKREFTDGPFTEAKEIIAGFWLIQVKSREEALEWASRCPLAEGEIV
                     EIRPVFENTDFPPEFRNKSA"
     gene            complement(2125..2541)
                     /locus_tag="VFR42_00895"
     CDS             complement(2125..2541)
                     /locus_tag="VFR42_00895"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0367732.1"
                     /translation="MRFMIMVRANKDTEADVKPDESLIAEMVTYHEELAKAGALVDAS
                     GLQPSSKGWRIRYSGKKRSVVDGPYTEAKELIAGYTIIEAKSREEALEWTKRFPNPFP
                     NRQESEIEVRPIKGLEELGDSEAVQRVLHLGVGTQK"
     gene            complement(2645..2926)
                     /locus_tag="VFR42_00900"
     CDS             complement(2645..2926)
                     /locus_tag="VFR42_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367733.1"
                     /translation="MTFQFTTVQALKTFAGAAIFGLGMDAASLASCHLTEALWFVLRE
                     AVGLVFWGVSTGWQASHAQVLGHNLLFLGCPLQMLHLLGSLAHFLASVV"
     gene            3126..4475
                     /locus_tag="VFR42_00905"
     CDS             3126..4475
                     /locus_tag="VFR42_00905"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU0367734.1"
                     /translation="MRKLAVPLTFLAGLCASTLAGAQEVQKPAAPADGGPLTGRWTVS
                     SDFHGTPLYFKLELAQEGEKLTGNFDGDKLEGTLNGSNIQFLAKDDHGGSEDAKGTIK
                     GNTISGTVVFTNGDDPTHPETHPFTATLVPAPPTGPPKRHDFVPTVFYRRFSPENKPV
                     LTVLPGDTIHTTTVDAGGTDEKGVSRVLGGNPETGPFYIETAAPGDTLVVHITKLRLN
                     RDWAISDDGIVDRAVDSDLAEKNKDLWKDVRWHLDIAKGIASPEKPKEHLTNYSVPVR
                     PMLGCVATAVGPAQAPPGTGDSGRFGGNMDFNEIVEGVTVYLPVSNPGALLYLGDGHA
                     VQGDGELNGNALETSMDVEFTVNVLPGKGIRGPRVESATHIMAMGLGGSLEDALRGAT
                     GSMAAWLNEEYKLTPSEVAQVLGSSAEYRISEVADRNAGVVLKISKERLKGLTAAAK"
     gene            complement(4481..5023)
                     /locus_tag="VFR42_00910"
     CDS             complement(4481..5023)
                     /locus_tag="VFR42_00910"
                     /inference="COORDINATES: protein motif:HMM:NF039902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367735.1"
                     /translation="MGTAELHPQTFENNFTAADVLQILREHDWLVADPTPDQTAWCER
                     SAALLGAKSPHRAGLENLLALVFHYDAREILSGMDAHLVLSRYAARDVLRKLANLLLE
                     TAPLTTERFNEIIEALKSELDIRGRELFHPLRLALSGRSGEGELDRVVLLLDDAAAAN
                     FSVPVKSARIRIIEFCSALD"
     gene            complement(5042..>5392)
                     /locus_tag="VFR42_00915"
     CDS             complement(5042..>5392)
                     /locus_tag="VFR42_00915"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU0367736.1"
                     /translation="AGLGDIGTHFPDTDPKWKGANSLAFLEHARKLLDERGLKIEHVD
                     AVVIMERPKLGPHFPQMREALAKSLRVTPETIHLKAKTNEGVDAVGRGEAIAAHAVAT
                     LYFDDRAFPCVVRA"
BASE COUNT         1062 a         1638 c         1517 g         1175 t
ORIGIN      
        1 ggtgaatgtc cgggttgtgc tggatgtccg gtgaagccac gtgaacattg tcacgcggaa
       61 gcaatcacta gcaaagcggt gcttaccgtt cgaagctggc accgctcgtg tggggtggtt
      121 caggacgcgg cgctgtggct ctgctccgcc ggaacttcgt agagctggcg cagttcgcat
      181 tcgccctggc ccactacgcg caaaaaaccc cgcacaagtt cgatggcgtc ctgtttcgac
      241 ttggcctgca gaatagcgaa tccgcccacg agttccttcg actccgtgaa cggcccgtcg
      301 gtgaccgtga agtttccgct ggctagccgc acccgcgcgc ccagcgcgct gggcaaacat
      361 ccttccgtgc ccaacagcca gcccgccctc attccttctt cgatgagttt ccccatcttc
      421 tccatttcct cctgcgtggg aggagcgttc ttttccgggg atttgtagat gctcaggaac
      481 cgcatgattc tctccttttt cgttcggcga gagttacatc tcgccgcatc gtttaccgcc
      541 gatttgcctg ctcttctata cgtcgaacca ctcgctccga aatcgacgca gatccaaaat
      601 ttatttcttt gcggccagtt ttttgcccag ttcgcgtgcc tgatcaatcg ctggactcgg
      661 cgcgaaatct tccagctcga aaaactgccg cacttcaatt tcgccttcgc cgttgggccc
      721 cggggccgga accttcttgg cccattccag ggcttcttgg cgtgacttta cgttgatgac
      781 ccaatagccg gcgatcagtt ccttgctctc cgcgaatggc ccgtcgacga tcgtgcgctt
      841 gccgccggaa aacttcacgc gaaaaccctt ggagcttggc tgcaggcccg acagatcgac
      901 caggacgccg gccttcagca gctcctcgtt gaatttaccc atcgtggcga tcatttcctc
      961 gctcggcatc actcccgctt ccgagtcctg gttcgctttt acgatcatca tgaaacgcat
     1021 gactttactc ctagtaagtt ggcttccggg ctgaggggtc cggcttaggc cacctgaatt
     1081 gaccttcact tagacgtcga atcgaaatat ccgatatcga cacgctgagc ggtggttggt
     1141 gtgctgggag cggaactagc gggccttctt agccttcaac ttggcttcgt tcttcgccac
     1201 tcgcgctttc accagttttt tgaccaaggc agccgggagt ggcttgtcca ccgcaaaccg
     1261 gatcgtcccc ttggagcagg gatagtcttt cagatcttcg gcgaattcct tcagcaactc
     1321 accgctcgcc ggaaagaagc tgcaatggtt ggcaaacgca gcgtaagcca ccagcattcc
     1381 gttgtatttg aatgccggaa tgccgtagct gatggcctcg gtggcttccc gtggagctgc
     1441 cgaccgtatc acggcgcgaa tcttttgcag cgtggttcgc gccggatggg ggaccctggc
     1501 gagatacttg tccacgtcgc cggaaccgga atgctgcttg gcggaattcc ccttggcgga
     1561 gggccgccgg ccggatttcg tcgtcttttt catgctgcgc acctgaagac ctggaatgtg
     1621 cggcgagttc ccgtcagtct atcagttgta attcgtaagc gaaacgctgc ttcgctcagc
     1681 cgtttaggcg gatttgttgc gaaattcggg cggaaaatcc gtattttcga ataccggccg
     1741 aatctccaca atttcgcctt cggcgagcgg gcatcggcta gcccactcga gcgcttcttc
     1801 gcgagacttc acttggatca gccagaatcc agcaattatc tctttggctt cggtgaacgg
     1861 cccgtccgta aattcccgtt tcccaccgga catcttgatg cgagtggctt tcgaactggg
     1921 atgcagtcct tccgccagca gcagcactcc ggctttccgc atgtcatcgc tatatttgcg
     1981 catcgccgtg accagttccc gcgtcggcac aaaattttcc tgcgcttcca ccggcgccgg
     2041 caatgacatc atgaatctca tcctagttcc ctttcgcctt cgctcttgct ttcgcttagg
     2101 tattccagac ccaacaacgc ggaactactt ctgcgtgccg acgccgaggt ggagaacgcg
     2161 ctgcaccgcc tcgctatcac caagctcttc gaggcctttg atgggtcgca cttcgatctc
     2221 tgattcctgg cggttgggaa aaggatttgg aaaacgcttg gtccattcca gtgcttcttc
     2281 tctcgatttc gcctcgatga tggtgtaacc ggcgatcaat tccttggctt cggtgtacgg
     2341 tccatccacc acgctgcgct tttttccgga ataccggatg cgccagccct tggagctcgg
     2401 ctgcagtccc gacgcatcca ccagcgcgcc agccttggcc agctcttcgt gatacgtcac
     2461 catttccgcg atcagacttt cgtccggctt gacgtccgct tcggtatcct tgttcgctcg
     2521 aaccatgatc atgaatcgca tcgtcttccc tcttccggtc tcagttttgc ccataggttc
     2581 ccattcagac gacgaatcgg gttcaacgga atcgacacgg ccccaactag aattccgaaa
     2641 aagctcagac caccgaagcc aggaagtgcg cgagggagcc cagcaagtgc agcatttgca
     2701 gcgggcagcc caggaaaagc aggttatgcc ccaggacttg tgcgtgcgac gcctgccagc
     2761 ctgtcgaaac gccccagaaa accagcccca ccgcctcgcg caacacaaac cacagcgctt
     2821 ccgtcaggtg gcaggaggcc aagctcgcgg cgtccatgcc caacccgaag atggcggctc
     2881 cggcgaaggt tttcagggcc tggactgtgg tgaattggaa tgtcatgttc gcctcctgcg
     2941 tgtttccctg ctttctgtct atatgacgaa ccagggtggc gaaaatcgac agtctggcga
     3001 gctttttcaa cagcaccagg aaccgtctac ccttgccgtc cgtatcttat tcacgcgaaa
     3061 ggactaacta ttcgccgtgc gtgtcgtcta ccatacttca cgaaaacgct cattgaggtg
     3121 aattcatgcg taagctcgcc gtccctctca cattccttgc aggactatgc gccagcacac
     3181 tggccggcgc gcaggaggtc caaaagcccg cggcgcctgc ggatggtggc ccgctaacgg
     3241 gccgttggac ggtcagctcc gacttccacg gcacaccgct ctatttcaag ctcgagctcg
     3301 cccaggaggg cgaaaagctc actggcaatt tcgacggcga caagctggaa ggcaccttga
     3361 acggctccaa cattcaattc ctggcgaaag atgatcacgg cggctcggaa gacgccaagg
     3421 gcaccatcaa gggcaacaca atttccggta ccgtggtttt taccaacggc gacgatccaa
     3481 cccatccaga gacgcatcct tttaccgcca cgctcgttcc ggctcccccc actgggccac
     3541 caaagcggca cgatttcgtt ccgacggttt tctatcggcg attctctccg gaaaacaagc
     3601 cggtgctcac cgttttgccg ggcgacacca ttcacaccac caccgtcgat gccggcggca
     3661 cggacgaaaa aggcgtgagc cgcgtgctgg gcggcaatcc ggagaccgga ccgttctaca
     3721 tcgagaccgc cgcgcccggc gatactctgg tcgtgcacat caccaaactg cgcttgaatc
     3781 gcgattgggc catcagcgat gacggaatcg tggatcgcgc agtcgatagc gacctcgccg
     3841 agaaaaacaa ggatttgtgg aaagatgtgc gctggcatct ggacatcgcc aagggaatcg
     3901 cgtcaccgga aaagcccaaa gaacatttga ccaactactc cgtgcccgtg cggcccatgc
     3961 tgggttgcgt cgcaacggcc gtcgggcccg cgcaagctcc gccaggaacc ggcgactccg
     4021 gccgctttgg cggcaacatg gacttcaacg aaatcgtcga aggcgttacc gtctacctgc
     4081 cggtgagcaa tcccggcgca ctgctgtatc tcggcgatgg tcacgccgtg cagggcgacg
     4141 gtgagctgaa cggcaatgcg cttgaaactt ccatggacgt ggagttcacg gtaaacgtac
     4201 tccccggcaa ggggattcgc gggccgcgcg tggaatcggc cacccacatc atggccatgg
     4261 gcctcggagg atcgctggaa gatgcgctgc gcggagcgac tggcagcatg gctgcttggc
     4321 tcaacgagga gtacaagctg acgccgtcag aagtagcgca agtgctggga tcctcggcag
     4381 aataccgcat cagcgaagtc gccgatcgca acgccggtgt ggtgctcaag atcagcaaag
     4441 aacgcctgaa aggattgacc gccgccgcga agtgactgca ttaatccagg gcggaacaga
     4501 attcgatgat gcggatgcgg gccgatttta ccggcactga aaaattggcg gcggcagcgt
     4561 cgtcgagcag cagaacgacg cgatcgagct cgccttcgcc ggagcggccg gatagtgcga
     4621 ggcgcagcgg atgaaacagt tcgcggccgc gaatatccag ttcggatttg agtgcctcga
     4681 taatttcatt gaaacgctcc gtagtgagtg gcgcagtctc gagcagcagg ttggcaagtt
     4741 tgcggagaac atcgcgcgcg gcatagcgcg aaagtaccaa atgcgcgtcc atgccggaca
     4801 gaatttcccg tgcgtcgtag tggaacacta aggccagcag attctccagc ccggcgcggt
     4861 gcggtgactt ggcaccgagt agcgcggcgg agcgttcgca ccaagccgtt tgatccggcg
     4921 tgggatcggc gacgagccag tcgtgttcgc gcaggatttg cagcacgtcg gctgcagtga
     4981 agttgttttc gaaggtttgc ggatgtaatt cagcagtgcc catggtgcga acagttctgc
     5041 ctcaggcacg caccacacac ggaaaggcgc ggtcgtcgaa gtacagtgtg gccacggcgt
     5101 gggcggcgat ggcttcgccg cggcctacag cgtcgactcc ttcgttggtt tttgccttca
     5161 agtgtatcgt ttcgggcgta acgcgcaggg atttggcgag ggcttcgcgc atctgcggga
     5221 aatgggggcc cagcttgggc ctttccatga tcacgacagc atccacgtgc tcgatcttca
     5281 gtcctctttc gtccaataat tttcgagcat gctcgagaaa cgccaggctg ttcgcgccct
     5341 tccacttggg atcggtgtcg ggaaaatgag tgccgatatc gccgaggccc gc
//
LOCUS       DASTPM010000051         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1270599, whole genome shotgun sequence.
ACCESSION   DASTPM010000051 DASTPM010000000
VERSION     DASTPM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270599"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VFR42_00920"
     CDS             complement(<1..1641)
                     /locus_tag="VFR42_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367737.1"
                     /translation="MTTQHTVRRKWRWLRHVAWVLGVKVALILIAAAVFFGSGAGNPL
                     LRRLAIKRINSITGGQTEIRSLSIQWFSMSATIKGFVIHGKEPGGTEPLFSAEEIHAG
                     LKIDSFWGRKVSLSEIGVRQPHIHIRAEKDGTTNVPAFPRASTASSKPLRETLLDLRV
                     RRFSLTDGWVLYNDVKTPLALEGHDLQLNFDAGGSMDHPLYTGALQWNDVLFTSKRFL
                     PVPLSASAKFTFWRNGLTIEQGQFGAGTSRFDVQAEMSDFSAPQWKYRYRAWLNLTDF
                     RKTFRAPLTPAGKVDLRGEGTFSGGELKGTGNFNGSEILLSYDILHQGGLHSTGDYRL
                     DKNGLDIANFAAYAFGGSVKGRVTMKLPGLEFRAQTRVDGMRIAQILPAIEHSGFPID
                     HLHWDSVLAADTVETWKGGFEHFQISGQTDWTAPDTVTPGHIPVTANWKFTYKQDPEI
                     LTLESALFETPQSRIQASGTLGHSDSILELHVRSDELEAFNDLIQSIEEVVPGTPEEV
                     VPRIHGAANWDGRMSGPLGGPTFTGHVRGERLAYGKFHA"
     gene            complement(1638..2243)
                     /locus_tag="VFR42_00925"
     CDS             complement(1638..2243)
                     /locus_tag="VFR42_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367738.1"
                     /translation="MFGSFFSLAQELIDRVVARIENDVILLSDVRLLSHYQLLVEGKT
                     ESDSEILDRLIDQWIVRNEANVARTPSPSDPDIDRAMQRLIQGFSSQQDFDARRKLAG
                     LTEADVRRITADQLFLNSYLDSRFRPTVQISEQAIQDFYKNSLLPRAQARGATPPAFD
                     TAHDFIQEALVQRGINEQADKWLKESHARLRVTKMLEESHP"
BASE COUNT          515 a          626 c          827 g          495 t
ORIGIN      
        1 tgcatggaat tttccgtaag cgaggcgttc gccgcgcaca tggccggtga aggtggggcc
       61 gccgagcgga ccgctcatcc tgccatccca attggcggcg ccgtgaatgc gcggcaccac
      121 ttcttccggc gtgccgggaa caacctcttc aatagactgg atcaagtcgt tgaacgcttc
      181 gagttcgtcg gagcgcacgt gcagttcaag gatggagtcg ctgtggccga gcgtgccgct
      241 ggcctggatg cggctttgcg gagtttcaaa cagggcggat tcgagcgtca gaatttccgg
      301 gtcttgtttg taggtgaact tccagttcgc cgtgacggga atgtgaccgg gagtgactgt
      361 atcgggagcc gtccagtcgg tttgcccgga aatctgaaag tgctcgaagc cacctttcca
      421 agtttcgacg gtatccgcgg cgaggacgga atcccagtgc aagtgatcga tggggaaacc
      481 gctgtgttcg atggcaggaa gaatctgggc gatgcgcatt ccgtcgacgc gagtttgcgc
      541 gcggaattcc aggcccggca gtttcatggt gacgcggcct ttgaccgatc cgccaaaggc
      601 gtaagcggca aagttggcga tgtcgagacc gtttttgtcg agcctatagt cgccggtgct
      661 gtgcagcccg ccttgatgaa ggatgtcgta gctgagcagg atttcagagc cgttgaaatt
      721 gcccgtgccc ttgagttcgc cgcccgaaaa cgtgccttcg ccgcgcaggt ccactttgcc
      781 ggcgggagtg agaggcgcgc ggaaggtctt gcggaagtcc gtgaggttga gccaggcgcg
      841 gtaacggtat ttccattgcg gcgcggagaa atcggacatt tcggcttgca cgtcaaaacg
      901 cgaagtaccc gcgccaaact ggccttgctc gatggtgagc ccgttgcgcc agaaggtgaa
      961 tttggcggag gcgctgagcg ggacgggcag gaagcgcttg ctggtgaaga ggacgtcgtt
     1021 ccattgcagc gcgccggtat agagcggatg gtccatgctg ccgccggcgt cgaagtttaa
     1081 ttgcaaatcg tggccttcca gggccaatgg agttttgacg tcgttgtaga gcacccagcc
     1141 gtcggtcagc gagaagcggc gaacgcgcaa atcgagaagc gtttcgcgca atggtttgga
     1201 ggacgccgtc gacgcgcggg ggaacgcggg aacattggtg gtgccgtctt tttccgcgcg
     1261 gatgtggatg tgcggctggc gcacgccgat ttccgaaaga gagactttgc ggccccagaa
     1321 ggaatcgatc ttaagtccgg cgtgaatttc ttctgcggaa aagagcggct cggttccgcc
     1381 aggttccttg ccatggatga cgaaaccctt gatggtggcg ctcatggaaa accactggat
     1441 ggaaaggctg cggatttcag tttggccgcc ggtaatggaa ttgatgcgtt tgatggccag
     1501 gcggcgaagc agaggattgc cggcgccgct gccaaagaag acggctgccg cgatcagaat
     1561 gagcgcaact ttgacgccca gcacccaagc tacatggcgc agccatctcc atttgcggcg
     1621 gacggtgtgc tgcgttgtca cgggtggctc tcctcgagca ttttggtgac gcgaaggcgc
     1681 gcgtggcttt ccttgagcca tttatcggct tgctcattga tgccgcgctg aaccagcgcc
     1741 tcttgaatga agtcgtgcgc cgtatcgaac gccggaggcg ttgcgccgcg agcctgagcg
     1801 cggggcaaca gggaattctt gtagaagtcc tgaatggctt gctcgctgat ttgtacggtt
     1861 ggccggaaac gcgagtcgag atagctgttg agaaaaagct ggtcggcggt gatgcggcga
     1921 acgtcggctt cggtcaggcc agcgagtttg cggcgcgcgt caaagtcttg ctgagaagaa
     1981 aagccttgaa tgaggcgctg catggcgcga tcgatatccg ggtcggaagg cgagggagtg
     2041 cgggcaacgt tcgcttcatt gcgcacaatc cactggtcga tcaagcggtc cagaatctcg
     2101 gaatcgcttt ccgttttgcc ttcgaccagc aactggtaat gggaaagcag gcgaacatcg
     2161 ctgagaagga tgacgtcatt ttcaatgcga gcaacgacgc gatcaatcaa ttcctgcgcg
     2221 aggctgaaaa aagagccgaa caagcatagg acggatagaa aaaaggtgcg ccgagttgga
     2281 acagcgccac gcatcagaag ctggacccca gactgaagaa aacctgcagg gccggcaggc
     2341 gcgtagacgt gaagccgagc gaaccggtcg ggcaagtggt gggcgtagtg ccactgggac
     2401 acggaatcgg aatgacaaag ttggggcgat tcaattgata acccaaatcg acgcgaatcg
     2461 ggc
//
LOCUS       DASTPM010000052         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_751692, whole genome shotgun sequence.
ACCESSION   DASTPM010000052 DASTPM010000000
VERSION     DASTPM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751692"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFR42_00930"
     CDS             <1..583
                     /locus_tag="VFR42_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367739.1"
                     /translation="GLLMGAITPVAIGFSLWGAHATKREVLIHLSALAAALLSLGIFF
                     VHYTWNPAVDCYDSVLVSRSAANYFHFAFLMFANYLGFDGLSSKHPEIWGGLFLLLLL
                     GIFGATVASISRSRKLLARPNTVQLVAFVLLAFSLLFCAATARGRLCLGFGAAQESRY
                     MPYLTPAFLGAYFYLGLHCLRAGCDTPCLAPAW"
     gene            868..1830
                     /locus_tag="VFR42_00935"
     CDS             868..1830
                     /locus_tag="VFR42_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0367740.1"
                     /translation="MKNNRRTFLKSAAGSAIASAAVPLFAKSAGFRFWSPDQQAPSVQ
                     FPTAPRERVAVASYPFRDFIAGARDPHATSSKMPLKEFAGHVRQKFNVNKIEPWSEHF
                     VSTDAAYLDELRSTVTKAGCGFANIAADGENSFYSPDSGVRAKAVAFGRKWIDIAAHI
                     GSPSVRINLPPARDTKPEAAVVAESLKVLAAYGASKNVVLHHENDNPLSENPFFIADV
                     LDRVSSPWDRALPDFGNSFSALPVEEAFRGLEQMFAHAYGISHVKDVSMTPKREAIAV
                     DLGRAFAIAKEHNYKGYFSMEWDTEGDPYAGTENLIVVTLKNIS"
     gene            complement(1856..2122)
                     /locus_tag="VFR42_00940"
     CDS             complement(1856..2122)
                     /locus_tag="VFR42_00940"
                     /inference="COORDINATES: protein motif:HMM:NF023510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3565 domain-containing protein"
                     /protein_id="HEU0367741.1"
                     /translation="MVWTNLPSRAQAVPVSEHPQRKIIAFHQDQHSDWVADLECGHTQ
                     HVRHNPPWQNRPWVVTPEGRSAYLGRELPCKKCAEEISFGLSAG"
     gene            2224..>2850
                     /locus_tag="VFR42_00945"
     CDS             2224..>2850
                     /locus_tag="VFR42_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367742.1"
                     /translation="MVTRAKAKKIAEPQAAQQHSPLTKFKHPWRIVPLHTAPRLAAAV
                     TPLLTYRNGPLLTNVEVFTVFWGAAWQDATNSATAKQMNEFFDFVLTSKLMDQLTEYN
                     VEGKTIGHGTRAGSLTLPTSEPGAKVQDSAIQKMLQAEIAAGTLPAKTANSLYFVFLP
                     PGTQVELSGSASCTDFCGYHDATSDNVFYAAMPYPNCTGCEGGLAELDA"
BASE COUNT          613 a          795 c          833 g          609 t
ORIGIN      
        1 cggcttgctg atgggcgcaa tcacacctgt cgccatcggg tttagcctat ggggagccca
       61 tgccacaaaa cgcgaagttc tgattcatct ttcagctctg gcggcggctc tgctttcgct
      121 tggaatcttc ttcgtgcatt acacctggaa cccggcagtg gactgctacg acagtgttct
      181 ggtttcacga agcgcggcca actattttca tttcgcgttt ctgatgttcg caaattacct
      241 cggtttcgac gggctgagca gcaagcatcc ggaaatttgg ggaggtttgt tccttttgct
      301 ccttctgggc atcttcggcg caacggtcgc gagcatatcg cgatcgagaa aattgctggc
      361 gagaccaaac actgtgcagt tggttgcgtt cgtcctgctc gccttcagcc tgctattttg
      421 cgcggcgacc gcgcgcggca ggctgtgcct cggtttcggc gctgcgcagg agtctcgcta
      481 catgccgtat ctcacacccg cgttcctggg cgcctatttt tacttgggtt tgcactgcct
      541 cagagctgga tgcgacacgc cgtgcttagc gcctgcctgg tgatttgtgc ttatgctggc
      601 tggcccgtcc acctgggcga tcagttggaa attacagggt ttcatgccgt caaactgcag
      661 tggaaaactt gctatttggc gagcaaggat atcgccacct gcaaccgcca gattggccgc
      721 cgaatttgca atgctcccga ggcgcccaat ttgaaggaga aactcaattt tctagagaaa
      781 gaacatctca atctgtttaa tggccgctaa gagcggcact gcaaaaagta tctaggcgcc
      841 acagacactg atgtacactt ggccgctatg aaaaacaacc gacgcacatt cttgaaatcc
      901 gccgctggtt cggcgattgc aagcgcagcc gtgccgctct tcgcaaagtc cgcaggcttc
      961 aggttttggt cgcccgacca gcaggcgccc tccgtgcaat tccccaccgc gccccgcgaa
     1021 cgagtcgctg tcgcttcgta tccattcagg gacttcattg cgggtgcgcg cgatccacac
     1081 gctacgtcgt ccaagatgcc gctcaaggaa ttcgccgggc acgtaagaca gaaattcaat
     1141 gtgaacaaga tcgagccgtg gagcgaacac ttcgtttcga cggacgcggc gtatctggac
     1201 gagctgcgca gcacggttac caaagccggt tgtggcttcg caaacatcgc cgcggacggc
     1261 gagaacagtt tttattctcc agattcagga gtgcgcgcaa aggcggtcgc gtttggaagg
     1321 aagtggattg atattgcggc gcacattggg tcgccgagcg tgcggatcaa ccttccgccg
     1381 gccagagaca ccaagcccga agctgcggtg gttgcggagt ccttgaaagt gctggccgca
     1441 tatggcgcga gtaaaaacgt cgtactgcac cacgagaacg acaacccgct tagcgaaaac
     1501 ccctttttca ttgcggatgt gctggaccgg gtgagcagtc cgtgggaccg ggcgctgccg
     1561 gacttcggca acagtttctc ggctctcccc gtcgaggaag cattccgcgg gctggagcaa
     1621 atgttcgccc acgcttatgg catctcgcac gtaaaagacg tttccatgac gccgaagaga
     1681 gaagcgattg ccgtggatct ggggcgcgcg tttgccatcg cgaaagagca caactacaaa
     1741 ggctactttt cgatggagtg ggatacggaa ggcgatccgt atgccggtac ggaaaatttg
     1801 atcgtcgtta cattgaagaa tatttcgtga ggggagaatt caaagccagg gaagttcagc
     1861 cagcgcttag gccaaaagaa atttcctcgg cacatttctt gcaaggcaat tcgcggccaa
     1921 ggtaggccga gcggccctca ggagtaacga cccacggccg gttttgccag ggcggattgt
     1981 ggcgaacatg ctgggtgtgg ccgcactcga ggtcggcgac ccaatcgctg tgctggtcct
     2041 gatgaaaggc gataattttg cgttgcggat gctcgctcac cggaacagct tgcgcccgcg
     2101 aaggtagatt cgtccagacc attccgtacc atttacagcg cacgcgcatc gcgctagcgt
     2161 gaagtttcta gccgcagccc tcccagcagg ccagacattt ccacaaatcg gggaggagcc
     2221 accgtggtaa cacgagccaa agcaaagaaa atcgcagaac cccaagcagc gcagcagcat
     2281 tctccgctca ccaagttcaa acacccgtgg cgaattgtgc cgctgcacac cgcgccgcga
     2341 ctggccgcgg ccgtcactcc gctactcacg tatcgcaacg gcccgttgct tacgaatgtg
     2401 gaggttttca cggtgttttg gggcgcggca tggcaggatg cgacaaactc cgccacggcg
     2461 aagcagatga acgagttttt cgactttgtg ctgaccagca aactgatgga tcaactgacc
     2521 gaatacaacg tggaaggtaa gacgatcggc catggaacgc gggccggctc gctgacgctg
     2581 ccgacttcgg agccgggcgc gaaagtgcag gactcggcga ttcagaaaat gctgcaagcg
     2641 gagattgccg ccggaacgtt gccagctaag actgctaatt cgctttattt tgtgtttttg
     2701 ccgccgggga cgcaggtgga attgagcgga agcgcgtcct gtacggactt ttgcggatat
     2761 cacgacgcga ccagcgacaa tgttttttac gcggccatgc catacccgaa ttgcacggga
     2821 tgcgaaggcg ggctggcgga gctggacgcc
//
LOCUS       DASTPM010000053         6676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_377081, whole genome shotgun sequence.
ACCESSION   DASTPM010000053 DASTPM010000000
VERSION     DASTPM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377081"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2480
                     /locus_tag="VFR42_00950"
     CDS             <1..2480
                     /locus_tag="VFR42_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367743.1"
                     /translation="MALVAFSSSATPTTAKVGFLLDLNKDTQTCLSNGAQTTPPCVHA
                     QVTLNTGTYPQIAMVPHQHLAYVTPGGTGVINGIDVTKSSSQLQITNIALISGLVTVT
                     VNVPSGQTLGLNPGNPGSVLIQGVPPGSSNNTNFNGVFSVQSVLNSNSFTYALNSSVN
                     DNATGDSDSLVFFSSPNVVVAISQTAQGITFNPITRTLAIADANATGSNGPQINLLRS
                     ADLQISSITLRADCTSATTSCSGAPELLGTSSVAFQPYSNLLVSYNPQQNQVSISNPV
                     TLQRTAFVCNLSSGSSGCLTNPTTNTDLGTFQGQIKLPGTGTSQVTVTAGGNSTTLNL
                     FGGLAVDPATNQAFVAQSGSNQILIVNLGPTPSTTLKPAQITELQVPTDPRAIIGGIP
                     GALMPQGTLTSNADLTNVQIFGSGFGTAGNSTTQVRLDGVSINGTCGSGPCVSVVSDR
                     QINATIPASFLASPHRYSVDIVNGSVGSNSTDFFVIKAVDLKPVCSGGNPQPSSVAIA
                     DQLPGQSFSPIAVVSNSGCGNVAVIDINPASATFGGVKSTIATGNDPLGVAVSPRFGL
                     AVVANNTDGTASVLDLRTGTQKVAAVAVGAQPTGVAISEDTGAALIANTGANTVSEIN
                     LALLFGSSPATSLSATTIGVDTSPIAVAIDPDRGTNNRGLAVITALQLISGSTAVGVL
                     DAVDIGGTTPAKATTGAVGGSVTATPTGIVFDPTVSPRLFYATSSGGNVVTSFNPDNG
                     ATSSVHVGINPSSLAINPQTGGIMTINFSSQTVSIIDTISNPFTTRRTYGLGGSAQFG
                     IAIDQFTNLVVLADQANNRVLIFPLPN"
     gene            2796..3410
                     /locus_tag="VFR42_00955"
     CDS             2796..3410
                     /locus_tag="VFR42_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU0367744.1"
                     /translation="MFLTAALAAASLAGSASLAAQSAPVAGSTANTKVGVLNVRQAIV
                     ATAEGKQASTELQGQFASRQTELENMNKQINDIRQRLQANEGKLSPEEGQRLQRQGEA
                     LAKQLQRKQEDYQEDVNASQQEVIDRIGRKMMDVLDRYARENAYTAIFDSSAQGAPIY
                     VSNGVDVTPEIVKLYDQAYPAKASATAAKPAATKPAATTAAPKP"
     gene            3613..4113
                     /gene="rimP"
                     /locus_tag="VFR42_00960"
     CDS             3613..4113
                     /gene="rimP"
                     /locus_tag="VFR42_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU0367745.1"
                     /translation="MQLEKIREAAERVARSEGLEVVDVEWKVGKQRFLRVYIDRLPAS
                     AADTDGQPGPYPKSSHADCERVSQQLSVILDVEELVPGPGYVLEISSPGMDRALLKQA
                     DYERFTGRSAKIWTEEPVEEMSFFEGRLAGITESKVKLELKGKTPRTVEVPLALIRKA
                     HLVVEF"
     gene            4244..5785
                     /gene="nusA"
                     /locus_tag="VFR42_00965"
     CDS             4244..5785
                     /gene="nusA"
                     /locus_tag="VFR42_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU0367746.1"
                     /translation="MANLLYQQIEMLSREKHIEPEVVVSAIEDAMVVAARKYYKTEED
                     LRGKFNQETGQVDVFSVVTVVEEVGDPTREISLTDARRKNPAAEVGTEILTSKPTDVL
                     GRIAAQTAKQVILQKVREAERDTIFNEYHNRVGELVTVIVKRAEGPDLIVDMGRTEAR
                     LPKREQSKLETYNIGERLRVVIRMVDRAAKGPQVIVSRADAALVQRLFEMEVPEIYDG
                     TVQIRAAAREAGERTKVAVFSRDKDVDPVGACVGMKGMRVQSIIRELRGEKIDIIPYS
                     EETVAFAQKALSPAKVTRVQITDPESHKLEVIVEDSQLSLAIGKKGQNVRLASKLIGW
                     DIDIKSEEEKRREIEAEMTALTAPVTPLTALAGVGPKTIEKIEAAGITSLEQLAGMTP
                     EQLMEIPGIGEKMVDKIYQAVNQFYESGAGAAPAEGAAEATEESESSENQTETASKTD
                     TEAPTVEIAGASMEANSGTVAEGEVETQLPPEAAAEPSTDSEPAGSETESVKEDEKRN
                     SDTNS"
     gene            5819..>6676
                     /locus_tag="VFR42_00970"
     CDS             5819..>6676
                     /locus_tag="VFR42_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU0367747.1"
                     /translation="MTDANQVRINELARELEVKAKAIIDLLPGFGVAEKKTHSSSIPV
                     DVAEKVRRTIQGQAEADAAVEAAAVAEKATKEAAARAARQRPAGPPPPVGAKPPAPAP
                     QAPVVAKPAAPAAAAPAPAPQPPAPAVPAPAAPVSVAPAAPPKPAPVSTPPASPVQAP
                     AAPPVAAKPAAPAPAAPQIQAPPSTPTAPKPVPAAPLGQPLRPAAQPGQGGAPGQRPQ
                     PRPGQQPSQPGAVRPAAAGQPRPSGQPPRAMPTGNRGPLPTGNRGPLPTGNRGPLPSG
                     DRPSGPRPGQ"
BASE COUNT         1416 a         2074 c         1920 g         1266 t
ORIGIN      
        1 acatggcact ggttgcgttc agttccagcg ccacgcccac cacggccaag gtcggcttcc
       61 tgcttgacct gaacaaagac acgcagacct gtctgtccaa cggagcgcaa accacgccgc
      121 cgtgcgtgca cgcccaggtc acactgaaca ccggcactta tccgcaaatc gccatggtgc
      181 cgcaccagca cctcgcttat gtgacacccg gcggcaccgg cgtcattaac ggaatcgacg
      241 tcaccaaatc ttcttcacag ctgcagatca ccaatatcgc cttgatctcc ggcctggtga
      301 ccgtcacggt gaatgtgccg agcggccaga ctctcggcct caaccccggc aatccggggt
      361 ccgtcctgat tcaaggcgtg ccgccgggaa gttcgaacaa caccaacttc aatggtgttt
      421 tttcggtgca gagcgttttg aactccaaca gttttaccta cgcgctgaac agttcggtga
      481 acgacaatgc aacgggcgac agcgacagcc tggtgttctt cagcagtccg aacgttgtag
      541 tcgccatctc gcagaccgcg caaggaatca cttttaatcc gattacgcgg acgctggcaa
      601 ttgccgacgc gaatgccacc ggcagcaacg ggccgcaaat caatttgctc cgctccgccg
      661 atctgcagat ttcttccata acgcttcgcg cagactgtac ttcggcgact accagttgtt
      721 ccggagcgcc cgaattgctg gggacaagtt ccgtggcctt ccagccttat tcgaacttgc
      781 tggtttccta caatccgcag cagaatcagg tatccatctc taacccggtt acgctgcaac
      841 gcaccgcttt cgtttgcaat ctgtcatccg ggtcatccgg gtgcctgacg aatccgacaa
      901 ctaacactga cctgggcact ttccagggtc agatcaagct gcccggaacg ggaacgtcgc
      961 aggttactgt aacggccggg ggcaactcga cgactctgaa tttattcggc ggcctcgccg
     1021 tggatccggc caccaatcaa gcgttcgtgg cgcagtcggg cagcaaccag attctgatcg
     1081 tcaatctcgg gccgacccct tcgaccacgc tgaagccggc gcagattacg gaactgcagg
     1141 tgcctacgga tcctcgagcg atcatcggcg gtattccggg cgccctgatg ccgcagggga
     1201 ctttaacttc caatgccgat ctaaccaacg tgcagatttt cggctccggc ttcggaactg
     1261 cgggcaattc gacaacgcag gttcgtctcg atggggtctc gattaatgga acatgtggct
     1321 ccggcccgtg cgtctcagtt gtgagcgacc gtcagattaa cgcaaccatc ccggcatcgt
     1381 ttcttgccag cccacatcgc tactccgtcg atattgtcaa cgggagcgtt ggttccaact
     1441 cgaccgattt ttttgtcatc aaagcagtcg acttgaagcc ggtctgcagc ggcggcaacc
     1501 cgcagcccag ctctgtcgcc attgccgacc aattgcccgg acagagcttc tcgccgatcg
     1561 cagtcgtcag caacagcggg tgcggtaacg tcgctgtgat cgacatcaat ccggccagcg
     1621 cgaccttcgg cggcgtcaaa tccacgatcg ccacgggcaa cgatccactg ggcgtggccg
     1681 tcagcccgcg cttcggcctg gcggttgtcg ccaacaacac cgacggcact gcttccgtgc
     1741 ttgacctcag aaccggaacg caaaaagtgg cggcggttgc cgttggtgcg caacccaccg
     1801 gggttgccat tagcgaggac accggcgcgg ctctgatcgc gaacaccggc gccaacacag
     1861 tttccgagat caatctggcg ctattgtttg gttcttcacc ggcgacatca ctttcggcga
     1921 ctaccattgg tgtggacaca agtcccattg cggttgcgat tgatccagat cgcggcacca
     1981 acaatcgtgg tctcgcggtg atcaccgccc tgcaactcat cagcggttcc acggccgtcg
     2041 gcgttctcga tgccgtggac atcggcggca ccacgcctgc caaagccacc accggcgcgg
     2101 tcggcggaag cgttaccgct actcccaccg gaattgtttt cgacccgacc gtttcgccgc
     2161 gtcttttcta cgcgacttcc agcggtggaa acgtggtgac gtcgttcaat ccggataatg
     2221 gcgccacgtc ttcggtgcac gtcggcatca atccgagttc gctggcaatc aatccgcaaa
     2281 ccggtggcat catgaccatc aatttctcca gccagaccgt ttcgataatc gacaccattt
     2341 ccaacccgtt caccacgcgg cgcacttacg gtttgggagg ttctgcgcag tttggtatcg
     2401 cgatcgacca gtttacgaat ttggtcgttt tagcggacca ggccaataac cgggtcttga
     2461 ttttcccgct gcccaattag gcgccgatct tcgatgggct agtgggattg ggtacaactt
     2521 gacgcggcat tcggcgcatc gcatgtgaca ggacattcct gctctgcctg ctcacagcca
     2581 tgcgctttgc cgttgctcgg gagggtttgc ctcggctata ctttttggtt tgtacggggc
     2641 ctgcgaatcc cgcagagcgc ggcgaaaccg ggtttcagca aaaagggttc agggttggtt
     2701 tagcaagtcc tgaggcagaa aattttcggc caatctaggt tggcgtaagg gaagtgctaa
     2761 gcgcttcctc caataaggag actactgtga ataagatgtt tcttacggcc gctctcgccg
     2821 cagcgagcct cgctggctcg gcttctctcg ccgcccagtc ggcgcctgtg gccggttcga
     2881 cggctaacac gaaagttggt gtcttgaacg tgcgtcaggc gatcgtcgcg acggccgaag
     2941 gcaagcaagc ctcgacggag ctgcaaggcc agttcgcgtc gcgtcagacc gagctcgaga
     3001 acatgaacaa gcagatcaat gacattcggc agcgcttgca ggccaacgag ggcaagctca
     3061 gcccggaaga aggccagcgg ctgcaacggc aaggagaagc gcttgccaag cagctgcaac
     3121 gcaagcagga agactatcag gaagacgtga acgcgtcgca gcaggaagtg attgaccgca
     3181 tcggccgcaa aatgatggat gtgctcgatc gctatgcccg cgaaaacgcc tacacggcga
     3241 tcttcgattc ctccgcgcag ggcgctccga tttacgtttc gaacggtgtc gacgtgacac
     3301 cggaaatcgt caagctgtac gaccaggctt atccggccaa ggcttcggct actgcggcga
     3361 agcccgctgc caccaagccg gccgccacaa ccgccgctcc caagccgtaa atcttttgga
     3421 atcagggtat tgccgagggc gctgcgaacc gggcgccctt ctttttatcc cttaccttgc
     3481 ttttgccgta ccgtgggcat atactttggt ttcgtccgtt acgggtcgca cgatcgagtg
     3541 ggcgcaagcc cgcttttttg tgtctgccgg ttgtagtggg cgtttcaccg gcattggccg
     3601 tggagtctct gcttgcagtt ggaaaaaatt cgtgaagccg ccgagcgggt tgcccggtcg
     3661 gaagggctgg aagtcgttga cgtcgaatgg aaagtcggca aacagcgctt ccttagggtg
     3721 tatattgacc ggcttccggc ctctgcggcg gacaccgacg gtcagcccgg cccttatccg
     3781 aagagttctc acgctgactg cgaacgtgtc agtcagcaat tgagcgtgat cctcgatgtc
     3841 gaggaactgg ttccgggtcc tggttacgtg ctggagatca gttctccagg catggaccgt
     3901 gctctcctga agcaagcgga ctatgaacgc ttcaccgggc gttcggcaaa aatctggacc
     3961 gaagaaccgg ttgaagagat gagttttttt gagggacgtc tcgcgggcat tacggagagt
     4021 aaagtcaagt tggaattgaa aggcaagact ccgcggactg tggaagtgcc gctggccctg
     4081 atacgcaagg cgcacctcgt cgtcgagttt tagtaaacac tcttcgtcgt tgttgtggct
     4141 gccgcgccct cgtcggcgcg ctagcgcggt agttacgctg attttacgcc gggttgctcg
     4201 gtttcaccgg cacaggagaa acaagctcgt tcgggaaagt gttatggcga atctgctcta
     4261 tcaacagatc gaaatgctca gccgcgaaaa gcacattgag ccggaagtgg tggtgtccgc
     4321 gattgaagac gccatggtcg tcgcggcccg caaatattac aaaaccgaag aagacctgcg
     4381 cgggaagttc aaccaggaaa ccggtcaggt cgacgtgttc tctgtcgtta ccgtcgtcga
     4441 ggaagttggc gaccccaccc gcgaaatcag cctgaccgat gcgcgccgca aaaatccggc
     4501 cgccgaagtc ggcacggaaa ttctcacctc caaacccact gatgtgttgg gccgcatcgc
     4561 tgcgcaaacc gccaaacagg tgattctgca aaaagtgcgc gaagcggagc gcgacacaat
     4621 ttttaacgag taccacaacc gcgtcggcga actcgtcacc gtcatagtca agcgcgcaga
     4681 aggtcccgat ctcattgtgg atatgggccg cacggaggcg cgtttgccca agcgcgaaca
     4741 gtccaagctg gagacctaca acatcggcga gcgcctgcgc gtcgtcatcc gtatggtcga
     4801 ccgggctgcg aaaggtcctc aggtcatcgt ctcgcgcgcc gatgccgctc tcgtccagcg
     4861 cctgtttgaa atggaagttc cggaaattta tgacggcacg gtacagatcc gcgccgccgc
     4921 ccgtgaagca ggcgaacgca ccaaggtcgc tgtgttcagt cgcgataagg atgtggaccc
     4981 agtcggcgct tgcgtcggca tgaagggcat gcgcgtgcag tccatcattc gcgaactgcg
     5041 cggcgaaaag atcgacatca tcccttacag cgaagaaacc gtggccttcg cgcaaaaagc
     5101 gctgagccct gcgaaagtaa cgcgcgttca gatcaccgac ccggaaagtc acaagctaga
     5161 agtgattgtg gaagattcgc aactctcgct agccatcgga aaaaagggcc agaacgttcg
     5221 cctggccagc aagctgatcg gctgggatat cgacatcaag agcgaagaag agaagcgccg
     5281 tgagatcgaa gcggaaatga ccgcgcttac cgcgccggtg actccgctga ccgcgctcgc
     5341 cggtgttggt cccaagacga tcgaaaaaat cgaagcggcc ggaattactt ctcttgagca
     5401 actcgccggc atgacgcccg aacagctgat ggaaattccc ggaatcggcg aaaaaatggt
     5461 ggacaagatc taccaggccg taaaccagtt ctatgagagt ggcgccggcg cggctcccgc
     5521 ggaaggcgct gcggaagcaa cagaagaatc cgagagcagc gaaaatcaaa ccgagaccgc
     5581 gtcgaaaaca gacacggaag caccaacggt tgagattgcg ggcgcgtcga tggaagcgaa
     5641 ctcgggcacc gtggcggaag gcgaagtgga gacgcagctt cctcccgaag ctgccgccga
     5701 acccagcacc gactcagaac cagccggcag cgaaacagaa agcgtcaaag aagacgagaa
     5761 gagaaacagc gataccaatt cgtagttatt gcagcattag tggtagtgaa cggagcgcat
     5821 gaccgacgcc aatcaagtac gaatcaacga gctggcccgc gagctcgaag tcaaggccaa
     5881 ggccatcatt gaccttctgc ccggcttcgg cgtcgcggaa aagaaaacgc actcgagctc
     5941 catccctgtg gatgtcgcgg agaaagtacg ccgcaccatt cagggccagg ccgaagcgga
     6001 tgctgcggtc gaagctgcag ccgtagccga gaaggccacc aaggaagctg ccgcgcgcgc
     6061 tgcgcgtcaa agacctgccg gtccccctcc accggttggc gccaagcctc ccgctcctgc
     6121 tccacaagct cctgttgtag caaaaccagc ggctccggcc gccgcggcgc cagcaccggc
     6181 tccacagccg ccagcgccgg cagtgccagc tcccgctgct ccggtttccg tggctcccgc
     6241 ggcgccgccc aaaccggctc ccgtctcgac accaccggca tcacctgtgc aagctccggc
     6301 tgcgccgcct gttgccgcca agcctgcagc tcccgcaccc gctgcgccgc aaattcaagc
     6361 tccgcccagc actcccacgg cgcccaaacc ggtacctgct gctccactcg gtcagccgtt
     6421 acggcccgcg gcccagcccg gccaaggcgg cgcgcctggc cagcgtccgc aacctcgtcc
     6481 cgggcaacag cccagccagc ccggcgctgt gcgtccggct gcggccggcc aaccgcgacc
     6541 ttcggggcaa cccccgcgtg caatgcccac gggcaaccgc ggtcccctgc cgacggggaa
     6601 tcgcggaccc ttgcccactg gcaatcgcgg cccgcttcct tccggtgatc ggcctagcgg
     6661 cccgcgcccc ggccag
//
LOCUS       DASTPM010000054         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1385984, whole genome shotgun sequence.
ACCESSION   DASTPM010000054 DASTPM010000000
VERSION     DASTPM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385984"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1202
                     /locus_tag="VFR42_00975"
     CDS             111..1202
                     /locus_tag="VFR42_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0367748.1"
                     /translation="MKVLVQPEDGLNPIVSAITRAKKMVEIVIFRLDRNLIEEAIRAA
                     ASRGVFVRALVAFANRGGEQQLRQLETRFLDSGVTVTRTSGDLVRYHAKMMIIDRKTL
                     YVLSFNYTTLDIEHSRAFGIITRNKALVQEAVKLFEADTKRHPYTPQLDNFLVSPLNA
                     RKQLAEFIRKARKELLIYDPKISDSQMVRLLLERQKAGVEIKIIGRLGKKAGSLNARR
                     LGTMRLHTRTIIRDRKHAFVGSQSLRKLELDSRRELGLIVKERAVVSKLVETFEGDWT
                     TNEEKETKSKVAGREKDKPGEMDRLAKKLDPLSESIKRAVKKVVKENGHKIVADDETK
                     ETLKQAVKKTVKQAFKEAVQETAARVESA"
     gene            1199..1813
                     /locus_tag="VFR42_00980"
     CDS             1199..1813
                     /locus_tag="VFR42_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367749.1"
                     /translation="MIPGARLWLALCPLLLMAGARSMACGQSASEAVQEKGPHGKQER
                     FREFLARVNEYDKLRNSVRGGIPDAGKRATAEQIQKHQEMLAEKIQDARKDARQGEVF
                     TADSQVAFRRAIDRAYSGKRANKIERTLVQGEPVKLDLYINKPYPEKIPTTTVPPTLL
                     QHFPKLPKKIEYRIVGNDLVLEDTESHLVIDIFPGAFPNAPPHT"
     gene            1818..>2595
                     /locus_tag="VFR42_00985"
     CDS             1818..>2595
                     /locus_tag="VFR42_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0367750.1"
                     /translation="MLRILIRVLLGAMVVVPAAAQQVTLPQAKGSVRFAIIGDSGTGE
                     RPQYETAERLRLAHEQFPFDFVLMMGDNIYGGKTPQDFQRKFEQPYQPLLDNKVTFYA
                     SLGNHDVPEEQSYKLFNMDGRRYYSFKKGNVRFFALDSNYMDPDQLTWLTNQLQGSTD
                     EWKIAFFHHPLYSSAAAHGSSLELRGILEPLFQQYGVQVVFSGHDHVYERMTPQKGSA
                     YFTEGSSGQLRRGNIRGAEFSARSFDTDCAFMLVEIISNEL"
BASE COUNT          644 a          659 c          800 g          492 t
ORIGIN      
        1 tgaaccgatt gtagccttac ggagagaggc cctagcctgc atgaccggta cgaaattggg
       61 ctattgctgc gtttgggtca ggtaggaccg agaacggagg aacttcgcct gtgaaggtac
      121 tggtacaacc agaagacggt ctgaacccca tcgtaagcgc gattacccgc gcaaaaaaaa
      181 tggtggagat tgtgattttc cgcctagacc gcaacctgat tgaggaagcc attcgagcag
      241 cggcttcgcg gggcgtattt gttcgcgcac tcgtggcctt tgcgaatcgc ggcggagagc
      301 agcagctgcg gcagctggaa acgcggtttc tggattccgg cgtgacggtg acgcgcacca
      361 gcggcgacct ggtgcggtat cacgcgaaga tgatgatcat cgacaggaag acgctgtacg
      421 tcctctcttt caactacaca acgctggaca tcgaacatag cagggcattt ggaatcatta
      481 cgcgaaacaa ggcgctggtg caggaagcag tgaaactgtt cgaggcggat accaagcgtc
      541 atccgtacac tccgcaactg gacaattttc tggtgagccc gctgaacgcg aggaaacaac
      601 tggctgagtt catccgcaag gcgcgcaagg agctgttgat ttacgatcca aagatttccg
      661 attcgcagat ggtgcggctg ctgctcgagc gccaaaaggc cggggtagag atcaagatta
      721 tcgggcggct gggcaagaag gccggttcgc tgaatgcgcg gcggctcggg accatgcgcc
      781 tgcatactcg gacgatcatt cgggatcgta agcatgcttt tgtcggcagc cagagcctgc
      841 gcaagctgga actggattcg cgccgcgagc tggggctgat cgtgaaggag cgagccgtag
      901 tcagtaagct cgtcgagacg ttcgaaggtg attggacaac caacgaagaa aaagaaacca
      961 aatcgaaagt ggcgggccgc gaaaaagaca aacccgggga gatggacaga ctggccaaaa
     1021 agctggatcc gttgagcgag tccatcaagc gcgccgtgaa gaaggtggtc aaggaaaatg
     1081 ggcacaagat cgttgcagac gatgaaacca aggaaacatt gaagcaggcg gtgaagaaaa
     1141 cggtgaaaca ggcattcaag gaagcggtgc aagaaacggc agcccgggtg gaaagcgcat
     1201 gatcccgggt gcgcggctct ggctggcatt gtgccccttg ctactgatgg ccggcgcgcg
     1261 aagtatggcg tgtggccaga gcgcgagcga ggcggtgcag gaaaaaggtc ctcacggcaa
     1321 gcaggaacgc tttcgggaat ttctggcaag agtgaacgaa tacgacaagt tgcgcaactc
     1381 agttcgcggg gggatcccgg acgccggtaa gagggccacg gcggaacaga ttcaaaagca
     1441 ccaggaaatg ctggcggaga aaattcagga cgcccgcaag gatgccaggc agggagaagt
     1501 tttcaccgca gattcccaag tggcgttccg ccgcgcgatt gaccgagcgt actccggaaa
     1561 acgggcgaat aagatagagc gcacgctggt gcagggcgag ccggtgaagt tggatttgta
     1621 catcaacaag ccgtatccgg agaagatacc gacgactacg gtgccgccga ccttgctgca
     1681 gcatttcccc aaattgccga agaaaatcga ataccgaatt gtgggtaacg atctggtcct
     1741 cgaggacacg gagtcccacc tagttattga tatttttccg ggcgcattcc cgaacgcgcc
     1801 gccgcacacc tgaagccatg ctgcgaatcc tgattcgcgt tctcctgggc gcaatggttg
     1861 tcgtccccgc tgccgcgcag caagttacgc tgccgcaagc taagggatcg gtgcgttttg
     1921 ccatcatcgg ggattcgggt accggcgagc ggccgcagta tgagacggcg gagcggctgc
     1981 ggctggccca cgagcagttc ccttttgatt tcgtgctgat gatgggcgac aacatttacg
     2041 gcggcaagac accgcaagac tttcagagaa aatttgagca gccctaccag cctcttttgg
     2101 acaacaaagt cacgttttat gccagcctgg gcaatcatga cgtaccagag gaacagtcct
     2161 acaaactgtt caacatggac gggcgccgct attactcgtt caaaaaaggc aatgtacggt
     2221 ttttcgcgct ggacagtaac tacatggatc cggatcagtt gacatggctg acaaatcaac
     2281 tgcaaggctc gacggacgag tggaagattg cgttttttca ccacccgctt tactcgtcgg
     2341 cagcggcgca cgggtcctcg ctcgaactgc gcggaattct ggagcccctt tttcagcaat
     2401 acggcgtgca ggtggttttc tccggacacg accacgttta cgaacggatg actccgcaaa
     2461 aagggagtgc gtacttcacg gaaggctctt ccggacaact gagacgagga aatatccgcg
     2521 gtgcagaatt cagtgcgagg tcgttcgata ccgactgcgc gttcatgctg gtcgaaatca
     2581 tatcgaacga gcttt
//
LOCUS       DASTPM010000055         4041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_607661, whole genome shotgun sequence.
ACCESSION   DASTPM010000055 DASTPM010000000
VERSION     DASTPM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607661"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1174
                     /locus_tag="VFR42_00990"
     CDS             248..1174
                     /locus_tag="VFR42_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEU0367751.1"
                     /translation="MPEERNPNGKNGKSVGLLNRIKSGVKEDLDSLKTDFPAKAKDQV
                     ETLKKPTKTQVYTSIFRHKHDDTPRNRALGVLSNVFLHLHPAKINRDAVRYSYTWGMG
                     GITFYLFIVLTFTGVLLMFYYHPSKVQAFRDVLYLEHDVPFGKLLRNMHRWAAHLMVI
                     AVELHMLRVFLTGSYKKPREFNWNVGVILLVLTLLLSFTGYLLPDDQLGFWAVTVGTN
                     MARATPLLGHEGPFGAELGMTPYNDVRFGLLGGSIVDANALLRSYIWHCIGIPIIASV
                     FMIVHFWRVRKDGGISGPAQVILESEVKEAQR"
     gene            1177..2106
                     /locus_tag="VFR42_00995"
     CDS             1177..2106
                     /locus_tag="VFR42_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367752.1"
                     /translation="MDWNQLWQIVSAPDNVPIVALIPLLAFYLFLAWRQAKANDTLMA
                     QLAGDAALAKTHHRKAWPFQSGWQREVHVWPFLLRMEFLAAIIVTIILMVWSIGLNAP
                     LEEPANPNLTMNPAKAPWYFLGLQEMLVYFDPWIAGVVMPTLIIFGLMVIPYIDTNPL
                     GCGYYTWKQRKFAISTFLFGFVILWVSMIFIGTFIRGPGWQWFWPGQTWDHNRVIYEV
                     NRDLPDLFGINSNTAKSLFGAVVVGGYYVAFGLVVHALFRRYNAKNYRRMSLLQYSLM
                     MFFLLTMFGLPIKILLRHSLHVKYVWITPWFNI"
     gene            2127..>4041
                     /locus_tag="VFR42_01000"
     CDS             2127..>4041
                     /locus_tag="VFR42_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367753.1"
                     /translation="MAEKPVPESDPIVSKSLAPHYVVALVVLIGTLFWALWDEDFGQR
                     PWKSFQQEWKDRYLAFLISARSKSSASQSDVENSGQYQTLKTGYESAMRETSPRVREI
                     NEKLHDLSAKILAVQNVFTDRRAFVTALTYSIETEASAPSRQRKQKALSQYKQEITSV
                     TFPDGSKQNYSYDQLEETYNDLKNERTKLSAELGEVLKPLNEQKEKLDAFLSERMVDL
                     TPEQIRGLQNKTEAWDSKIVQINVAEANIVDRCESCHLGIREPVKLSAVSMSVKGKKP
                     DKYARVFTSHPESDLLKTHDAEKFGCSPCHQGNGRATTSVEKAHGTYEHWLWPLFPKG
                     NAEAGCQTCHAADMVLVNNEVGWTLSEGKNLFQQRGCVGCHRYEGYDKEPEDLLLTAQ
                     QIKQLEQEKKDSVKQAAYLIKQADAAATNEEASRLNERAVALKVSNSKLELRLVQLYR
                     RTKSLLQDMKKIGPNLKDVRLKLNKNWIPVWLEKPSAFRATTKMPDFRLNDDQVKAIS
                     SYLWQSALTDSLPKHKLGSATHGRELFETRGCLACHSIGEGDQLQGGTFAANLTRVGE
                     KDNFDYLVRWVHNARERTRPYCLLEKRDIGPEDYAKKGLPFVFDLEHSRCPNDGHELQ
                     VQNMTVMPSLRLSP"
BASE COUNT          960 a         1132 c         1089 g          860 t
ORIGIN      
        1 cgacaacgaa ggcgtgaact ttgagggacc tgcgccaagg ccgatggatc gcgctcacgt
       61 ggagttagca cccgatggtc agatcgtcgt ggatgtttca cgactctatc aatggccaaa
      121 aggccaagca acccacttca acgacgcagg atcgtatttg gtggcttgac gcggccacgt
      181 ggcgcgcaaa acggcgagga gagctagaga acaaatccga acggcgcgaa gacgggagac
      241 cacttaaatg cccgaagaga gaaacccgaa cggcaagaat ggcaaatccg tagggctgct
      301 taaccgcatc aaatcgggcg tgaaagaaga cctcgactct ctcaagacag attttcccgc
      361 caaagccaaa gaccaagtcg agacgctaaa gaaaccgacc aagacccaag tctatacttc
      421 gatcttccgc cacaagcacg acgacacgcc gcgcaatcgg gccttgggag ttctgtctaa
      481 cgtcttcctg catctccatc ctgcaaaaat caaccgcgac gccgtgcgct atagctatac
      541 ctggggaatg ggtggaatca ctttctactt gtttatcgtg ctcaccttta ccggcgtttt
      601 gctgatgttc tattaccacc ctagcaaggt ccaagcgttc cgggatgtgc tctacctcga
      661 acatgacgta cccttcggta aattactccg caacatgcac cgttgggccg cgcacctgat
      721 ggtgatcgct gtcgaattgc acatgctccg agtcttcctc accggctcct acaaaaagcc
      781 gagagagttc aattggaatg tcggggtcat tctgctcgtg ctcactctgc tgctatcttt
      841 caccggatat ttactgccgg atgatcaact cggtttctgg gcggtgaccg tgggcacgaa
      901 catggctcgc gccactcctc tgctcggcca cgagggtccg ttcggcgcgg agttgggcat
      961 gactccttac aacgacgttc gcttcgggct gctgggcggc tcaattgtcg atgcgaacgc
     1021 cttgctccgc tcctatatct ggcattgtat tggaattcca atcattgcgt ccgtcttcat
     1081 gattgtacac ttctggcgtg tgcgaaagga cggcggtatc tccggcccgg cgcaggtgat
     1141 tctcgaatcc gaggtcaagg aggcgcagcg ctagccatgg attggaatca attgtggcag
     1201 atcgtttccg cgccggacaa cgtgccgatc gtcgccctca ttcctttgct cgccttctat
     1261 ctttttctgg cttggcgtca ggctaaggca aacgacacgc tgatggcgca gctcgcggga
     1321 gacgcggcgt tagccaaaac ccaccacaga aaggcctggc cattccagtc cggctggcaa
     1381 agagaagttc acgtctggcc gttcctcctg cggatggaat tcctcgcggc gattatcgtg
     1441 accatcattc tcatggtctg gtccattggc ttgaatgcgc ctctcgaaga accggcgaac
     1501 cctaacctga ccatgaaccc tgccaaagca ccctggtact ttctcggcct gcaagagatg
     1561 ttggtttact ttgacccatg gatcgccggt gtggtcatgc cgacgctgat catcttcggt
     1621 ctcatggtga tcccgtatat cgacaccaac cctttgggtt gcggctacta cacctggaag
     1681 cagcgcaagt tcgccatctc tacatttcta ttcggttttg tcattttgtg ggtttcgatg
     1741 attttcatcg gcacctttat ccgtgggccc ggctggcaat ggttttggcc gggacagact
     1801 tgggaccaca accgcgtgat ctacgaagtg aaccgtgatc tgccggatct attcggaatt
     1861 aattccaaca cggcaaaaag tctttttggg gccgtagtgg tcggtggcta ctacgttgcc
     1921 tttgggctcg tcgtgcacgc cttgttccgc cgctacaacg ccaagaatta ccggcgcatg
     1981 agcctgctgc agtattcgct gatgatgttt ttcctgctca cgatgtttgg tttgccgatc
     2041 aaaattctgc tgcggcattc gcttcacgtg aaatacgtat ggatcacgcc atggttcaac
     2101 atttagcgcg agcctaggag gagctggtgg ccgaaaagcc tgtgcctgaa agcgacccaa
     2161 tcgtcagcaa atcgctggcg ccgcactacg tggtggcgct ggtcgtgttg attggcacat
     2221 tgttctgggc cttatgggat gaggacttcg ggcagcgccc ttggaagtcc ttccagcagg
     2281 agtggaaaga tcgctatctg gcatttctga tcagcgcccg ctccaaatca agtgcttcgc
     2341 agagtgacgt ggaaaacagt gggcagtatc agacgctaaa gacaggttat gaatcggcga
     2401 tgcgcgagac gtctcctcgc gttcgagaga tcaacgagaa gttgcacgac ttgagcgcaa
     2461 aaattctggc cgtgcaaaac gttttcactg accgacgcgc cttcgtcacg gctttgacct
     2521 acagtatcga aaccgaggcg agtgcgcctt cgcgacaacg caaacaaaaa gcactcagcc
     2581 agtacaagca ggaaatcacc agcgtgacgt ttccggacgg cagcaaacaa aactacagct
     2641 acgatcagct cgaagagacc tacaacgatc tgaaaaacga gcgcaccaag ctcagcgcgg
     2701 aactcggcga ggtgctcaag ccgctcaacg aacaaaaaga aaaactggac gcctttcttt
     2761 ccgaacgcat ggtggacctg acacctgagc agattcgcgg cctgcaaaat aaaactgaag
     2821 cctgggattc caaaatcgtc cagatcaacg tagccgaggc gaacatcgtg gatcgctgcg
     2881 agtcctgcca cctgggaatc cgcgagccgg tgaaacttag tgccgtttcg atgtcggtca
     2941 aagggaaaaa acccgacaaa tacgcgcggg tgttcaccag ccatccggag tctgatcttc
     3001 tgaagactca cgacgccgaa aaattcggct gttctccatg ccatcaggga aatggccgcg
     3061 ccacgacaag cgtggaaaag gcgcacggca cttacgagca ctggctgtgg ccactgtttc
     3121 ccaaaggaaa tgcagaagcc ggctgccaga cctgtcacgc cgccgatatg gtgttggtca
     3181 acaatgaagt cggctggacg ctcagcgaag gcaagaatct gttccagcag cgcggctgcg
     3241 tcggatgcca ccgctacgag ggatacgaca aagagccgga agatctgctt ttgaccgcac
     3301 aacagatcaa gcaactcgag caggagaaga aagacagcgt caagcaagcg gcctatttga
     3361 taaaacaagc cgatgccgcc gcgaccaacg aagaagccag ccggctcaac gagcgcgccg
     3421 tcgctttgaa ggtctccaac agcaagcttg aactgcgcct tgttcagctc tatcgcagga
     3481 ccaaaagcct gctgcaagac atgaagaaga tcggcccaaa cctgaaagat gttcgcctca
     3541 agttgaacaa aaattggatt cccgtatggc tcgaaaaacc gagcgctttc cgcgccacca
     3601 ccaagatgcc ggacttccgc ttgaacgacg accaggtcaa ggccatctcg tcctacttgt
     3661 ggcagtcggc gctcaccgat tctctgccca aacacaaact gggaagtgcg acgcacggaa
     3721 gggagctgtt tgagacgcgc ggatgcctgg cgtgtcactc cattggtgag ggagatcaat
     3781 tgcagggtgg aacatttgcc gcgaacctta ctcgtgtcgg tgagaaggac aacttcgact
     3841 accttgtgcg ctgggttcac aatgctcgcg agcgcacgcg tccgtattgc ttgttggaga
     3901 aaagagacat cggccccgaa gactacgcga aaaagggcct gccctttgtt tttgatttgg
     3961 agcacagccg ctgcccaaac gacggtcacg aactgcaagt gcagaacatg acggtgatgc
     4021 ccagtcttcg tctctccccg c
//
LOCUS       DASTPM010000056         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_694168, whole genome shotgun sequence.
ACCESSION   DASTPM010000056 DASTPM010000000
VERSION     DASTPM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694168"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFR42_01005"
     CDS             complement(<1..527)
                     /locus_tag="VFR42_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047215127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0367754.1"
                     /translation="MQNVTLIGIDLGKHSFHVHGQDRAGRAVFRRKFSRKQLVEFFAT
                     FHACTVVMEACAGAHWMAGKLSQFGHTVKLISPQFVRPFVKSNKNDFVDAEAICEAST
                     RPSMRFVSPKTESQQTLAALHRVRQALVRDRVRTTNQMHGFLLEFGISLPVGHAVVKR
                     LAVVLAEYQLPPRLVA"
     gene            921..1559
                     /locus_tag="VFR42_01010"
     CDS             921..1559
                     /locus_tag="VFR42_01010"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU0367755.1"
                     /translation="MHGLLRERNGSLFEVIHSLRKAYPIAAMCALAGVSRSSYYAFVR
                     PREQPRRASHLLDAARQIHDASRASYRSRRMAQSLQRKGFAVGRYKARSLMREAELAV
                     RKRRTHRYRKAEGEAQVAQHLLERKFEPGAINRVWAGDVTYVRSKQGWSYLAIVMDLH
                     SRRIVGWAFDTQADTELVIQALQHAYRSRKPRAGLMFHPDQGRPIYERALCR"
     gene            1828..>2645
                     /locus_tag="VFR42_01015"
     CDS             1828..>2645
                     /locus_tag="VFR42_01015"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0367756.1"
                     /translation="MNDQSIRSEVILGVDTHLDAHVGAVISEAEKLLGTLSVPADAAG
                     YLKLLIWANSFGCLLRAGVEGTGTYGAGLTSVLRDHEIDVLEVNRPDRSTRRLRGKSD
                     PTDAENAARSVISGSATAIPKTQSGAAEAMRAVSVARRSAVKAKTQAINQLRALLVSA
                     PQETRERLWKGKPAECVDGCARVRSLGSTPLLQTLTTTLRLLAKRWLMLTEELRTLDA
                     MLDRLTSEHASRLRERFGVGPQTAAVLVAVAGDNPERLKSEAALAALCEASPLQA"
BASE COUNT          584 a          726 c          819 g          516 t
ORIGIN      
        1 gcaacgaggc gcggcggtag ttgatattcg gccaatacga cggcgagccg cttcacaacg
       61 gcgtggccaa ctggcaggct tatgccgaat tcgagcagga atccgtgcat ctgattggta
      121 gtgcgtactc ggtcgcgaac caaggcctgg cggacgcggt gcagtgctgc aagggtttgc
      181 tgcgattcag ttttcgggct cacgaaccgc atcgaaggtc gcgtagaagc ctcacaaata
      241 gcctccgcgt caacgaaatc gttcttgttg ctcttgacga acgggcgtac gaattgcggt
      301 gagatcagct tgacagtgtg tccgaactgg ctaagcttgc ccgccatcca gtgagctcca
      361 gcgcatgcct ccattaccac ggtgcacgcg tgaaacgttg cgaagaactc caccaactgc
      421 ttgcgcgaga acttcctgcg aaacacggct ctgccggctc tatcctgccc atgcacgtga
      481 aaagagtgct tgcctagatc aatcccgatc aacgtgacgt tctgcatgga agtgccctcc
      541 ggtggaaatt atctccagcc tactccgcga taggcggggg tgaccatccc attacaggaa
      601 cgatcaagag ttaacagccc ccaaacatca ttttgtaaag acttctaaat gaggtaagaa
      661 aatgggcaga cgaagttttc cagatgaaat gaaggccgat gtaatccggc tggtcgtgga
      721 gcagcgatat ttctttgctc aggcgagcaa ggcaacgggc gttggcgaca cggcttgacg
      781 gcgctgggtg gcgcaatggc gcgcgcaaaa accggcgccc ccacgcacag ccgaccagat
      841 tagcgccgat acgcagcgca taaaggagct ggaagcgcgg gtcgccgatc tcgaaaggga
      901 gcgtgagatc ttaaaaaaaa atccacggcc ttcttcgtga aagaaatgga tcgctctttg
      961 aagtgattca ttcgttgagg aaggcctatc cgattgccgc catgtgcgct ttggcgggcg
     1021 tatcgcgcag cagttactac gcattcgtgc gcccacggga acaaccccgg cgggcatcgc
     1081 acctgcttga tgcagcgcgt cagatccacg acgcaagccg tgccagctac cgcagccgca
     1141 ggatggcgca gtcgctgcag cgcaaaggtt tcgcagtcgg gcgctacaag gcgcgcagct
     1201 tgatgcggga ggcagaactc gcggtacgca agcgtcgtac gcatcggtat cgaaaggctg
     1261 aaggcgaggc tcaggtggcg cagcacctgt tggagcgcaa gttcgaaccc ggcgcgatca
     1321 atcgcgtctg ggccggggat gtgacatacg tgaggagcaa gcagggctgg tcgtacctgg
     1381 ccatcgttat ggacttgcac tcgcgccgca tcgtaggttg ggcgttcgat acgcaagccg
     1441 acacggagct ggtgattcag gctttacagc acgcgtatcg gagccgaaaa ccgcgcgctg
     1501 gactcatgtt tcatccggat cagggccgcc caatatacga gcgcgcgctt tgtcgatgat
     1561 ctgaaaaaag ctggcattac acaaagcatg agccgccgag gtaattgttg ggacactcag
     1621 tctaagatcg gccctgaacg tcggaccgat ctgacccgtg ctgggattgg ccgaactgcc
     1681 ttggtgttga cctgtcgatc gggttccggc gttccccgag cgttgccggg cccagcgtct
     1741 gtgacctgat aggagctttg ttctgcaccg tgagtgtcct gtcctgcgaa tggggttggc
     1801 gatgcgttca ctacaaagga agctcgcatg aacgaccaat cgataagaag cgaagtcatt
     1861 ctgggtgtcg atacgcatct ggatgcacat gtcggcgcgg tgatcagtga agcagaaaag
     1921 cttctcggga cgctgtcggt gcccgccgat gcagccggat atctgaaact attgatctgg
     1981 gccaattcat tcggctgtct gttgcgcgcc ggggttgaag gaacgggtac gtacggagct
     2041 ggcttgacca gcgtattgcg ggaccacgag attgatgtgc tggaagtgaa tcgcccagac
     2101 cggagcacgc gccgactccg gggaaagtcc gatcccacgg atgccgagaa tgccgcgcga
     2161 tcggtcatct ccggcagcgc aacagcgatt ccgaaaacgc agtccggcgc agccgaagcg
     2221 atgcgcgctg tctctgttgc cagacgcagt gccgtgaagg cgaaaacgca ggccatcaat
     2281 caattgcggg cattgctggt cagcgctccc caggaaactc gcgagcgcct gtggaaagga
     2341 aagccggcag aatgcgtcga cggttgcgca cgtgttcgtt cgttgggatc aacgccactg
     2401 ctgcaaacgc taacaacgac gttgcgtttg ctggccaaac gctggctcat gttaaccgaa
     2461 gagctcagga cgcttgatgc gatgcttgat cggctgacta gcgagcacgc cagccgcctt
     2521 cgtgagagat tcggtgttgg tccacagacc gcggcagtgc tcgtcgccgt agcgggcgac
     2581 aatcccgaac gtctgaaaag tgaagctgca ttggcagcgc tttgtgaagc gagcccgctg
     2641 caggc
//
LOCUS       DASTPM010000057         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_146269, whole genome shotgun sequence.
ACCESSION   DASTPM010000057 DASTPM010000000
VERSION     DASTPM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146269"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VFR42_01020"
     CDS             complement(<1..1044)
                     /locus_tag="VFR42_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU0367757.1"
                     /translation="MSFTPDDRGTELRALFFESANELLQALNEAGLELEARPADEEVI
                     RSVRRAVHTLKGDSAACGFSKLSEVAHELEDILNPQMGQLRGAKFAEVVLVAADSFEA
                     MLSAYQRDAEPPNMDALRAMIYQLRDAPVSSAAVAGSVTVLPAKFDWSEYEHLMISEA
                     IHRGETVFNVALRVDQSSGMRAAAFQLIRNVLHGSGTVIALRPEDNSTAANVDLVEAA
                     LATAHTQQQLEQKCEIPSIVSQVLVEIASHAKTAEHELLADLLDAHAAKVNSAIAESA
                     ENPGSGKPGALGSAVAAVAESTLRVDAARIDSVMNLVGELIIGKSMLNRALTEFDRVH
                     SKDPVRAKLADALA"
     gene            complement(1041..1946)
                     /locus_tag="VFR42_01025"
     CDS             complement(1041..1946)
                     /locus_tag="VFR42_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEU0367758.1"
                     /translation="MGTLTAQIQLTEAELKLLQTLVYQECGMFFDERRSHFLKDRLQR
                     RLKVCQLDSFYSYYRLLTSREGRAELALLLENLTVNETSFFRNRPQLDLLQKTVLEEL
                     LRKKQERRDWTLRVWSAGCSSGQEAYSVAILICDALAYYYLRNPLPFDMPSPKPLIPP
                     PWKLEILASDISYASLRAGQEGLYSEQQMEPVDYTCRLRYFEKNGEKYGVKQQLKELV
                     QFDFHNLKTEFLPRRNDIILCRNVMIYFDEAEQKRLIDKFYNCLNPGGYLFVGHAESL
                     FGLTQKFRMVHENNGTAYQRLEVNG"
     gene            complement(1985..>2256)
                     /locus_tag="VFR42_01030"
     CDS             complement(1985..>2256)
                     /locus_tag="VFR42_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU0367759.1"
                     /translation="TDKKNRILVVELDGKLVGLIVNSASEVLKIPPSEIESPGSVFAD
                     NESSYVTGVGKLKGRLIILLDISKLLHRPEFKKLEEAAEPVAALR"
BASE COUNT          473 a          645 c          648 g          490 t
ORIGIN      
        1 ggcgagagca tccgcaagct ttgcgcgcac cggatctttg gagtgcacgc gatcgaattc
       61 cgtcagcgcg cggttcagca tggactttcc gatgatcaac tcgcccacaa gattcatcac
      121 cgagtcgatc cgcgcagcgt ctacgcgcag agtgctttcc gctacggctg ccacagcgga
      181 tcctagcgcg ccaggtttgc cggaccccgg gttttccgcc gactcggcga tcgcggaatt
      241 caccttggct gcgtgagcgt ccagaaggtc cgcaagcagt tcgtgttcgg cggttttggc
      301 gtgcgaagca atttcaacca gcacctgtga aacaatggaa gggatttcgc acttttgctc
      361 gagctgttgc tgcgtatgcg ccgtggcaag agcagcttcc accagatcca cattcgccgc
      421 ggtggaattg tcttccggcc gcaaagcgat gaccgtgccg ctgccgtgaa gcacatttcg
      481 aatcaactgg aacgccgcag cccgcatgcc gctggactgg tctacgcgca gcgcaacatt
      541 gaacacagtt tcgccgcgat ggatcgcctc cgagatcatt aagtgctcgt actcgctcca
      601 gtcaaacttg gccggcaaca ccgtgaccga gccggcgacg gcagcggagg aaacgggagc
      661 atcacgcaac tggtaaatca tagcgcgcaa cgcatccatg ttcgggggtt cagcatcgcg
      721 ctggtaggcc gaaagcatgg cctcaaatga atcggccgcg accaggacaa cctcagcaaa
      781 tttcgcgccc cgcaactgtc ccatctgggg attgaggatg tcctccagtt cgtgggccac
      841 ttcgctgagt ttgctgaaac cacaagccgc tgaatcgccc ttgagtgtgt gcacagcacg
      901 acgaacgctg cggatgactt cttcgtccgc cggacgagct tccagctcca gcccagcctc
      961 attgagagcc tggaggagtt cgttggcact ctcaaaaaag agcgcacgaa gctccgtgcc
     1021 gcgatcgtcg ggcgtaaagc tcaaccattc acctcaaggc gctgatacgc ggtgccgttg
     1081 ttctcatgca ccatgcggaa cttttgcgta agtccaaaaa ggctttcggc gtggcccaca
     1141 aaaagatagc cgccgggatt caagcagtta taaaacttgt cgatcaggcg cttttgttcg
     1201 gcttcgtcaa aatagatcat gacgttgcgg cagaggatga tgtcgttgcg gcgcggcaaa
     1261 aattccgttt tcaggttgtg gaagtcgaac tggaccagtt ccttgagctg ctgcttgacg
     1321 ccatactttt cgccgttctt ttcgaagtat cgcaagcggc aggtatagtc caccggttcc
     1381 atctgctgtt cgctgtagag accttcctgt ccggcgcgca aactggcgta actgatgtcc
     1441 gacgcgagaa tttccagttt ccacggcggc gggatcagcg gcttgggaga aggcatgtca
     1501 aacggcagag gattgcgcag gtagtagtag gccaacgcgt cacaaatgag tatggccact
     1561 gaatacgctt cttgacctga ggagcaaccg gcgctccata cccgcagagt ccagtcgcgg
     1621 cgttcttgtt tcttgcgcag cagttcttcc aaaacagttt tctgcagcag atccagctgc
     1681 ggccggttgc gaaagaaact ggtttcgttg acggtcaggt tttccagcaa cagggccagt
     1741 tccgcgcgcc cttcgcggct cgtaagcagg cggtagtagc tatagaacga gtcgagctga
     1801 cagactttca ggcggcgctg caggcgatcc ttgagaaaat gcgagcggcg ttcgtcaaaa
     1861 aacataccgc attcctgata aaccagcgtt tgcaggagct tcagctccgc ctctgtcaat
     1921 tgaatctgtg cggtaagcgt tcccatgcga atgtgcgcaa cgttgccgat tcccggagca
     1981 gcggttatct cagggcggcg acaggttccg ctgcctcctc gagctttttg aattcggggc
     2041 gatgcagcaa cttgctgatg tccagcagga tgatgaggcg gcctttcagc ttgccgactc
     2101 cggtgacgta actcgattcg ttgtcggcga ataccgagcc cggcgactcg atctccgagg
     2161 gcgggatctt gagcacttcc gaggcggagt tcacgatcaa gccgaccagc ttgccgtcga
     2221 gctccaccac caggatgcga ttttttttgt cggtga
//
LOCUS       DASTPM010000058         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_924863, whole genome shotgun sequence.
ACCESSION   DASTPM010000058 DASTPM010000000
VERSION     DASTPM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924863"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1076)
                     /locus_tag="VFR42_01035"
     CDS             complement(204..1076)
                     /locus_tag="VFR42_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367760.1"
                     /translation="MTVRTDTSRTSLLLPGVLALVILALSAADGVSAGEPRLRSDTVT
                     AFDEYVRLTDMRNDDELKRNANFLWIDGLPETARVQAYEALKRGEVKMEKLETRANGE
                     KISCPGGMIHHWVGVVFIPAAKLQTVLRMLQDYDHHAQIYAPDVERSKLESHNGDDFK
                     VFLRFRRHKVITVVLNTQHDVHYFRDSEAREHSRSSAVRIAEVENPGPSEHEKKPGED
                     GGFLWRMETWWRVEERDGGVYVQSEVASLTRDIPAGLGWLIGPFVNSIPRETLTFTLQ
                     ATRKAVVGQFPSIN"
     gene            complement(1526..1978)
                     /locus_tag="VFR42_01040"
     CDS             complement(1526..1978)
                     /locus_tag="VFR42_01040"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU0367761.1"
                     /translation="MTSLSKVFDPQTVLLAKHAQHVVLVHFPIGLFLTGVAFDWAARW
                     TRRQSLSEAAYYNLLVAAASTIPVVVTGLLAWQFQLEGQRLKGILLLHLLLGSASGLL
                     IWTVWWLHFKARRQATAMSSFLWVLEAFGVLVVGLTGHLGGFLAGVNG"
     gene            complement(1975..>2164)
                     /locus_tag="VFR42_01045"
     CDS             complement(1975..>2164)
                     /locus_tag="VFR42_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU0367762.1"
                     /translation="VGATVTWTNKDDIPHTSVSTDGVFKSKVLDTDEKFSFTFTKPGT
                     FPYFCSVHPKMTGKVVVQ"
BASE COUNT          461 a          640 c          615 g          448 t
ORIGIN      
        1 atgaggctct tagggaaaag ttggccgaag aagcagcgcg aactgccagc cttgaaacct
       61 gggatcacaa tgctgcgggc acttgggaat tcctgaaagc cacgctcgct agaaagcggg
      121 cgtgaaccgg tttgcccccg agcgtagcgg atccgtgcgc agctttcatg ctcggtttgg
      181 gattaccctc gttgtgtacg atattagttg atcgacggga actgccccac caccgctttg
      241 cgtgtcgcct gcaatgtaaa ggtcagcgtc tccctgggta tgctgttgac gaaggggcca
      301 atcaaccacc ctaatccggc tgggatgtct cgcgtgagcg acgccacttc gctttggacg
      361 tagacgccgc catcgcgttc ttccactcgc caccacgttt ccatgcgcca caagaagccg
      421 ccatcctcgc ccggtttttt ctcgtgttcg ctggggcccg gattctccac ttcggcaatg
      481 cgcaccgcgg aactgcggct gtgctcgcgc gcttcagaat cgcgaaaata gtgcacgtcg
      541 tgctgtgtgt tcaggacgac ggtaattacc ttgtgccggc gaaatcgcag aaagacctta
      601 aagtcatcgc cattgtgcga ctcgagtttc gagcgttcga catccggcgc gtaaatctgg
      661 gcgtgatgat cgtagtcctg cagcatccgc aggacagtct gcagcttcgc ggcggggata
      721 aacacgacgc cgacccagtg atggatcatc ccgccggggc acgagatttt ttctccgttg
      781 gcgcgcgttt ccagtttttc cattttcact tcgccgcgct tgagcgcctc ataagcctgc
      841 acgcgagcgg tttctggcag accatcgatc cacagaaaat tcgcattacg cttcaattcg
      901 tcgtcgtttc gcatatcggt cagtcgcaca tactcgtcaa aggcggtgac ggtatctgac
      961 cgcaaccgcg gctcgcccgc ggagaccccg tcggcggccg acaatgccag gataactaag
     1021 gccagaacgc ccggaagaag tagcgatgta cgcgaagtgt ccgtccttac cgtcaattgt
     1081 cctctaaatc gttcacgggg ccgtggtcgc ggtcccgtat ctccttgaaa acaaagacta
     1141 acatcctagc ctatgggagg cttggcgcga gaaaaggcaa ttcacgttcc tatcatttca
     1201 cgaatacgtc tctcgcgtgc aggaggagtt cgcttgagtg gaaaagtcag atactgcgga
     1261 ttggcctcgc gccaccgcac cggctggcgt gagaaggcag gagggaagga attggcgggt
     1321 tgggtagctc gcacacgacc ccttgcggag ccgctgtccc cgcgcggccc ccaacccgtt
     1381 ctttgcacat tacggattgc atctgcaaag gccaatgggc caccggagcc actcggcgcg
     1441 gtacgtgggt cgtggtacta cggctctact cgtcccaagg agcagggtgc tatatgctgc
     1501 tgataatacg gcacttgaat ctgttctagc cattcacgcc ggccaggaag ccgcccaaat
     1561 gcccggtgag cccgaccact aacaccccga aggcttccag cacccaaaga aatgatgaca
     1621 tggcagttgc ttgccgtcgc gccttgaaat gcagccacca taccgtccag atcagcaagc
     1681 ctgaggcaga gcccaagagc aggtgcagca acaggatgcc cttgaggcgc tggccctcta
     1741 actggaactg ccaagccagc aatccagtaa ctaccaccgg gatggtggag gcggcggcga
     1801 ccagcaggtt gtagtatgcc gcctcggaaa gcgactgccg cctggtccac cgtgcagccc
     1861 agtcaaaagc gaccccggtg agaaagagcc cgatcggaaa gtgcaccagc acaacgtgtt
     1921 gcgcgtgttt ggccagcagc acagtttgcg ggtcgaacac tttcgagagg ctggtcactg
     1981 cactaccacc ttgccggtca tcttggggtg caccgagcag aagtacggaa acgttccggg
     2041 cttggtaaac gtgaacgaaa atttctcgtc cgtatccagc accttggact tgaacacacc
     2101 atctgtgctg accgaggtat ggggaatgtc atctttattt gtccatgtga ccgtcgctcc
     2161 gacg
//
LOCUS       DASTPM010000059         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1212978, whole genome shotgun sequence.
ACCESSION   DASTPM010000059 DASTPM010000000
VERSION     DASTPM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212978"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..1187)
                     /locus_tag="VFR42_01050"
     CDS             complement(291..1187)
                     /locus_tag="VFR42_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0367763.1"
                     /translation="MKAATAKTRIQFRNILFATDFSPAAMRAIPIAKAIARNYDSNIV
                     ALHVRGPVVNPLTPPAAWPMVLEAEKQKDELRRGELLSYFAGVPAAARIEEGDILSHL
                     AEAIKNDNVDLVVVGTHGRTGFGKLILGSVAEEILRTVHCPVLTVGPNSGPAWATGTR
                     IREVLFATDFSPESREAAAYAVSLAQEFQARLVLLHVVANPEAGDLVSPADVTAASEN
                     LLRQFLPEDAELWCKPEFVVAHGKNAELILEIAKQRESDLIVLGARPESGVPGASTHL
                     PIATVHKVVTHANCPVLTVRHD"
     gene            1709..>2160
                     /locus_tag="VFR42_01055"
     CDS             1709..>2160
                     /locus_tag="VFR42_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0367764.1"
                     /translation="MRYGARVLFDDVTCTFMAGRRYAITGPNGAGKSTLMKILTGELE
                     PTKGNVARPKKLGVLSQDQFAFDGFRVIDTVIMGNASLWETLQEREALYAKPHDELTD
                     ADGMRLGELEGIVGEEGGYTAESDAAILLDGLDVPAELHERKMGELQGG"
BASE COUNT          426 a          590 c          691 g          453 t
ORIGIN      
        1 tagaaactag gccagaacgc atttctagcc gatcaggcgc atcggtttcg actttgttgc
       61 acctggtttt gctggctgct cggagtttgg aaatcgctat ctttgtggga cagcgccaac
      121 caggtgccct gccgtgccga acgaagtggc caaccggggt acaaccgtct gggtgtctag
      181 gtgctttcct gagcctgcat tattgattat tgcgttaagt atattgtgaa caaaagaagg
      241 gccggcgctc caatccggag cggtcggccc tgtgcagcgc ggtctgcggt tcagtcgtga
      301 cgaaccgtca gaaccgggca attggcgtgc gtcaccactt tgtgtaccgt cgcaatcggc
      361 agatgcgtcg acgcacccgg tactcccgac tccggacgcg cgccaagcac gatcaggtca
      421 ctttcacgct gttttgcaat ttccagaatc agctcggcgt ttttgccgtg cgcgacgacg
      481 aattccggct tgcaccaaag ctcggcgtct tccggcagga attgccgcaa gagattctcc
      541 gacgcggcag tcacgtccgc cggggagaca agatcaccgg cttccggatt ggccacgacg
      601 tggagcaaaa ccaggcgcgc ctggaactct tgcgccagtg acacggcgta ggcggctgct
      661 tcgcgtgact ctgggctaaa gtcggtcgcg aatagaactt cgcgaatcct tgtgccggtt
      721 gcccaagccg gaccggaatt cggtccgacg gtaagaaccg ggcagtgcac ggtgcgaagg
      781 atttcttctg caacggaacc gaggatgagc ttgccgaatc cggtgcgccc atgggttcct
      841 acgacaacga ggtccacgtt gtcattcttg atggcttctg cgaggtgcga aagaatgtct
      901 ccctcttcaa tgcgtgcggc ggctggaacg cccgcaaaat aggagagtaa ttcgccgcgc
      961 cgcagttcgt ctttctgctt ttcagcttcg agaaccatag gccaagcggc gggtggggtc
     1021 agcgggttca cgactggacc acgtacatgc aaggcgacga tgttggagtc atagttccgg
     1081 gctatggcct tggcgatagg gatcgctcgc atagccgcgg gagaaaagtc ggtggcaaat
     1141 agaatgttgc gaaattggat ccgggttttt gctgtggcgg ctttcatagg tccccctctc
     1201 gacctaagtt ttgaatcgct ggggggcagg agtctgtgac ggaagtcacc ggtcagagtg
     1261 actgcagaca cgtgcctggc cgaaagggct tgagtgcgtg aaggcaggta ggcgccagtt
     1321 tcgcgattgg cgccccgcca attttgttct ggaccgtctc cgcgggatgc tctactgccg
     1381 cgtccataaa cgatgtctgc accgggattc tgcgccaagc gcatcttttc tgttccgata
     1441 tcgatggcca caacatgcat gcccatggct ttagcgtact gaacggcgag gtggcctaaa
     1501 ccgcccacgg cggagatggc cacccattcg ccggagcgcg tctcggtttg ttttagtttg
     1561 tacgtggtga ccccggctct cgaattggtg cagcgggccg gaacgggacg gtgtcaccgc
     1621 aggccggaac agggcgtggc ttcgatttcg gagagtgtgg ggttccacgt taaaatagag
     1681 agctatgatt tcggtgaaca acgtaacgat gcgctatggt gcgcgcgtcc ttttcgacga
     1741 tgtgacctgc accttcatgg cgggccgccg ctatgccatt accgggccga acggcgcggg
     1801 caagtctact ttgatgaaaa tcctgaccgg cgaactggag ccgaccaagg gcaacgtcgc
     1861 gcggccgaag aaactgggcg tgctgagtca ggatcagttc gcttttgacg gcttccgcgt
     1921 gatcgacacg gtgatcatgg gcaacgcctc gctttgggaa acgctgcagg agcgcgaagc
     1981 gctatacgcc aaaccgcacg acgagctgac tgacgccgac ggcatgcgcc tgggcgaact
     2041 ggagggcatc gtcggcgaag aaggcggcta cacagcggag tcggacgccg cgattttgct
     2101 cgatgggctg gacgtgcccg ccgagttgca cgagcggaaa atgggcgagc tgcagggcgg
//
LOCUS       DASTPM010000060         4419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_434722, whole genome shotgun sequence.
ACCESSION   DASTPM010000060 DASTPM010000000
VERSION     DASTPM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434722"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1183)
                     /locus_tag="VFR42_01060"
     CDS             complement(83..1183)
                     /locus_tag="VFR42_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0367765.1"
                     /translation="MNTLLARPTCPNWLPFVMPASLLLLACVALAAFTQAQNDRPPWA
                     RKSKTANTSAPAADNSSSDVLQDRAKIKVKVDLVNVLVSVLDEHNRPAPNLPVEAFRL
                     FDEDNPQTIAIFEKETQQPLDLALMFDASLSTQLGQPAQREAATKFIQQVLRPGDRLA
                     VYAVDENVTQIAAFSDNVPRLQDAIKRIPQGAGTSIYDAVFLGSQALTRQGVDRRSVI
                     ILITDGGETTSHADFDTARREAVRNGTLLYTVVIRAMKNENGRNTAGEHALETITDTT
                     GGAVFYPDSTSQLGDIFDRINRELRTQYRLGFYPTPRGPADTYRHIEVSVVAPASPPS
                     PPAATEAATEQPSAPAGAYAVRHRKSYYTGLP"
     gene            complement(1188..1667)
                     /locus_tag="VFR42_01065"
     CDS             complement(1188..1667)
                     /locus_tag="VFR42_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU0367766.1"
                     /translation="MTAAQSEDRIEITRAVIPNAEIAVQVRTDGDGAVAAFDGCVRNQ
                     SHGRATLYLEYEAYEAMALAKMREIAAHIHANYAIRKVAMVHRLGRLEIGETSVFIAV
                     SAAHRAAAFDACRYAIDRLKKTVPIWKKEFFADGAVWADGELPPVPAETPAAKPASN"
     gene            complement(1692..1937)
                     /locus_tag="VFR42_01070"
     CDS             complement(1692..1937)
                     /locus_tag="VFR42_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0367767.1"
                     /translation="MTVKVLYFGRLKELSGTSEEIVEVWDGALIEDLFARCSTARPEL
                     SKFRTSLVAARNHEFAAWSTPLSAGDEVGFLPPVSGG"
     gene            2035..2955
                     /locus_tag="VFR42_01075"
     CDS             2035..2955
                     /locus_tag="VFR42_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU0367768.1"
                     /translation="MKILVAGATGLVGTALVGALAGDGHTVCRLVRPQTRTEGGAHGA
                     FDVAWNPETGELGGAAVGAEGVVNLAGASIADGRWTAARKRLLRTSRVDATRALVAVL
                     GKMNAKPSVLISASAIGFYGNRGDEVLTESSVPGSDFLSDLARDWEAEALRAETFRTR
                     VVLPRFGIILAKHGGALAKMMLPFRLGVGGRIASGQQWMPWVALDDVVEIIKFVLSTR
                     DVSGPINVVAPEPVRNVEFTRALAEALHRPALFPAPAIALRIALGHEMAEALLLSSHR
                     VVPAALTRAGYRFLHSQLGPVLKGILTSQR"
     gene            complement(2966..3496)
                     /locus_tag="VFR42_01080"
     CDS             complement(2966..3496)
                     /locus_tag="VFR42_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367769.1"
                     /translation="MKQNALLVMFVLAFSAVATAQETAKPKQDEHTPAVIPLRVQIVF
                     SEYDGEKKVANLPYTFTVNADERRAKPGSQIRTGARIPVTTGKDQFTYLDVGTNVDCS
                     ATLQDDGRYKLQMALERSSVSPDAQSAGSNPVLRTFRVDLNPVLKDGQSVESIVSADP
                     LNGHVYHVSVTLNVIK"
     gene            complement(3538..4179)
                     /locus_tag="VFR42_01085"
     CDS             complement(3538..4179)
                     /locus_tag="VFR42_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367770.1"
                     /translation="MNEAALSDERLPAEVQAHLASCASCRESFSDEKALFGLLEAEVR
                     MRINAEMPASLLPRVRHEIAALAAARTWRVPVPAYIASGLAVGAIALSFAVRTKVSSV
                     KPEPSAHNISLPAPNEPSVPQTGEGPVEMLVATSKRSHKQPQVALRAEPKVLVSAEEQ
                     VGLQRYTASLRSMAAERAANVKPEALLEIKHLEIAGVDLKGLSIDPLQSGDSD"
     gene            complement(4176..>4419)
                     /locus_tag="VFR42_01090"
     CDS             complement(4176..>4419)
                     /locus_tag="VFR42_01090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0367771.1"
                     /translation="RVASAGEGSGEFQRQLESALDQLPEKQRLVLLLAAMEGHTLDEV
                     SVLLGVPVGTVKSRLFFAKKALAEKLRCFVNPIEKR"
BASE COUNT          867 a         1317 c         1290 g          945 t
ORIGIN      
        1 tgcgggttcc aataaatgta ttgggaaagt caccacaact ggctggagtt tcctggctgc
       61 tcgatgcctc tcgccgggct ccctacggaa gccccgtgta ataagacttt cgatgccgca
      121 ccgcgtaagc gcccgccgga gcggaaggtt gctcggttgc tgcctctgtg gccgccggag
      181 gcgacggagg cgaagccgga gccacgaccg atacttcaat atgcctgtag gtgtccgcgg
      241 gcccgcgcgg cgtggggtaa aagcccaacc ggtattgcgt gcgcagttcg cgattgatgc
      301 gatcgaaaat gtcgcccaac tggctggtgc tgtcaggata gaaaaccgcg cctcctgtag
      361 tgtccgtaat ggtttcgagc gcgtgttcgc cggccgtgtt gcgcccattt tcgtttttca
      421 tggcgcgaat gaccacggtg tacagcagcg tgccgttgcg aaccgcttcg cgccgcgccg
      481 tatcaaagtc cgcatgactc gtggtttctc ccccgtccgt gatcaaaatg atgaccgagc
      541 ggcgatcaac gccctggcgt gtcaaggcct gcgagcccaa aaacacggcg tcgtagatcg
      601 aggtaccggc accttgcgga atgcgtttga tggcatcctg caggcgcggc acattatcag
      661 agaacgccgc aatctgcgtc acgttttcat ccacggcgta aaccgccagc cggtcgcccg
      721 gccggagcac ttgctgaatg aactttgtcg ccgcctcgcg ctgggcgggt tggccaagtt
      781 gcgtgctcaa gctggcgtca aacatcagcg ccaggtcgag gggctgctgc gtttctttct
      841 caaaaatagc gatcgtctgc gggttgtctt cgtcaaaaag ccggaacgct tccaccggca
      901 ggtttggcgc gggacggttg tgctcgtcga ggacgctcac cagcacgttt accaagtcca
      961 ccttgacctt gattttggca cggtcttgca agacgtcaga agaagaattg tccgcagctg
     1021 gcgcagaggt attggccgtt ttggacttgc gggcccaggg ggggcgatcg ttctgggctt
     1081 gggtgaaagc cgccagcgca acgcaagcca agagcaacaa gctcgccggc atcacgaacg
     1141 gcagccagtt ggggcaggtt gggcgcgcca gcagtgtgtt cattaattca gttggatgcc
     1201 ggtttcgcgg caggagtctc cgcgggcacg ggaggcagtt cgccatcggc ccataccgcg
     1261 ccatcggcga agaactcttt cttccagatg ggtacagtct ttttcaacct gtcgatcgca
     1321 tagcggcagg cgtcaaatgc agccgcacga tgcgcggcgc tcaccgcaat aaacacgctt
     1381 gtctcgccaa tctccagccg cccgaggcga tgcaccatgg cgactttgcg aatcgcatag
     1441 ttggcatgaa tgtgcgcagc gatctcccgc attttggcca gcgccatcgc ttcgtaggct
     1501 tcgtattcaa gataaagcgt cgcgcgcccg tgcgactggt tgcgcacaca gccgtcaaac
     1561 gccgcgactg cgccgtctcc gtccgtgcgc acctgcacgg caatctccgc gttgggaatc
     1621 accgcgcgtg taatttcgat gcgatcttcg ctttgagcag ccgtcatagc gctgtcggct
     1681 tagccgccgg tctagccccc actcacggga ggcaggaagc ccacttcgtc gccggcgctc
     1741 aatggcgtgc tccacgccgc gaattcgtga ttgcgtgcgg cgaccagcga cgtgcggaat
     1801 ttgctcaact ccggccgcgc tgtcgaacac cgcgcaaaca aatcttcgat caacgctccg
     1861 tcccacactt cgacaatttc ttccgaagtg ccggaaagct ccttcaatcg gccaaaatag
     1921 agcactttta cggtcattgg gattcgctgg tttattctaa cccgaattcc cgccaggggc
     1981 tgttcccacg aggacctgcc aacgactcat ctctcctagc ctatacttga gaccgtgaaa
     2041 attctggtag cgggagcgac aggactggtt ggcacggcgc tggtgggagc gctggccggc
     2101 gatggccaca ctgtctgccg actggtacgt ccgcaaacca gaaccgaagg cggggcacat
     2161 ggagccttcg acgtcgcttg gaacccggaa acaggcgaac tgggcggagc tgccgtcgga
     2221 gccgaagggg tcgtaaatct cgcaggcgcg tccattgcgg atggccggtg gacggcggcg
     2281 cgcaagcgtc tgctgcggac cagccgcgtg gacgcaactc gcgcactcgt cgcggttctc
     2341 ggcaaaatga acgccaagcc atcggtcttg atcagcgctt ctgcgatcgg cttctatgga
     2401 aatcgcggcg atgaagttct gaccgagtcc agtgttcccg gatcggattt tctctccgac
     2461 ctggcgcgcg attgggaagc ggaggcgctg cgggcggaga cctttcgcac gcgtgtcgta
     2521 ctgccgcgct ttggaatcat tttggccaag cacggcggcg cactagcgaa gatgatgctg
     2581 ccgtttcgtt tgggagtcgg cggaagaatc gccagcggcc agcaatggat gccttgggtg
     2641 gcgctcgatg acgtggtgga gatcatcaag tttgtgctca gcacgcggga cgtgagtggt
     2701 ccgatcaacg tcgtcgcgcc ggagccggta cggaacgttg aatttacgcg ggcgctcgcc
     2761 gaggcccttc atcgcccggc tctcttccct gctcccgcga tcgccctgcg gatcgctctc
     2821 ggccacgaaa tggctgaggc tttgctgctt tcgagccatc gcgttgtccc cgccgcatta
     2881 acgcgcgctg gctatcgatt cttgcactcg cagctcgggc cagtgctgaa aggcatcctg
     2941 accagccaaa ggtaagcctg gcgtcctact tgatgacgtt taatgtcaca ctgacgtggt
     3001 agacatgccc gttcagagga tcggccgaga caatgctttc caccgattgg ccatctttaa
     3061 gtactgggtt taagtcgacc ctgaaggtcc gcaagacggg attgctgcct gcggactgcg
     3121 catcgggcga caccgaactg cgttccagcg ccatttgcag cttgtagcgg ccgtcgtctt
     3181 gaagggtggc tgagcaatcc acgttggtcc caacgtcaag gtaagtaaac tggtccttgc
     3241 cagtggtgac cgggattcgc gcgccggtac gaatttgcga tccaggcttc gctcgccgtt
     3301 catccgcatt aaccgtaaag gtgtagggaa ggttggcaac cttcttttcg ccatcatact
     3361 cgctgaagac aatttgaacc cgcaatggaa tgaccgccgg agtatgttcg tcctgcttcg
     3421 gcttggcagt ttcctgcgca gttgcaacgg ctgagaaagc caggacaaac attacaagca
     3481 acgcattctg tttcatcgtc gactcctaat aatgaactcc gctaaatcca acctggttca
     3541 gtccgaatcg ccgctttgca gcggatcgat agatagaccc tttaaatcca cgccggcaat
     3601 ttctaaatgc ttgatctcaa gcagcgcttc cggtttcaca tttgcggctc gctcagctgc
     3661 catgcttcgt aaagatgccg tgtagcgctg cagcccgacc tgctcttcgg cggaaacaag
     3721 tacttttggt tcggcgcgta atgccacttg tggctgtttg tggctcctct tactggttgc
     3781 gacaagcatc tctaccggtc cttccccggt ttgcgggacg ctgggttcgt tgggagctgg
     3841 caaagaaata ttgtgagcag aaggctctgg tttcactgac gagactttgg tccgcacagc
     3901 aaacgagagt gcaatcgcgc caactgccag ccctgaagcg atatacgccg gaaccggtac
     3961 acgccaggtt cgcgccgcgg ctaacgcggc aatctcgtgc cgaactctcg gaagcagtga
     4021 cgcaggcatt tccgcattta tgcgcattcg cacttcggcc tctaataggc caaacaaggc
     4081 cttctcatct gaaaacgatt cacggcacga agcacacgac gcgagatgag cctgcacttc
     4141 agcgggcaat cgttcatcgc tcagtgccgc ttcgttcaac gtttctcgat aggattcaca
     4201 aaacatcgca gtttctccgc aagcgccttt ttcgcaaaaa acagccggga tttgaccgtt
     4261 cccaccggga cgcccagcaa aaccgaaact tcatccagcg tgtggccttc catggccgcg
     4321 agaagcagaa ccaaccgctg tttctccggc aattggtcca gcgccgattc cagttggcgc
     4381 tgaaattcgc cactgccctc tccggctgag gcaacgcgc
//
LOCUS       DASTPM010000061         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_780842, whole genome shotgun sequence.
ACCESSION   DASTPM010000061 DASTPM010000000
VERSION     DASTPM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780842"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1280
                     /locus_tag="VFR42_01095"
     CDS             180..1280
                     /locus_tag="VFR42_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0367772.1"
                     /translation="MQAVDRVKAGSRNRPVAVALQGGGAHGAFTWGVLDRLLEESDFE
                     IVGISGTSAGAMNAGILADGLRRGGPPEARAALARYWEDVGRLPGYASIAPTPSLATE
                     REWHLDRNPFFLWADMLARVWSPYQINPFNYNPLRGLLERIDFDGLRCDADAARVFIC
                     ATNVRSGLRRVFDNSELSVDVLLASACLPLMNQAVEIGGQHYWDGGYTGNPALAPLYL
                     KTAADDVIIIGINPLLRAEVPRTAREIISRIDEISFNSSFFMELGAIAFVDELLKAGA
                     PQNFVKLLFVHQICNEQLGSLGASSKMNNDPAFLRHLHTIGRQSAEDWLAEHREAVGK
                     ESTVDLTGLLPLQDGAFADPMGIRQTAAHTVS"
     gene            1475..2224
                     /locus_tag="VFR42_01100"
     CDS             1475..2224
                     /locus_tag="VFR42_01100"
                     /inference="COORDINATES: protein motif:HMM:NF004777.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0367773.1"
                     /translation="MMEKIILITGGGRGIGAACALLAAQQGYKVCVNYRSDGAAAFNV
                     VAAIEDVGGTALAIQADVSDEAAVERMFALVDERFGRLTALINNAGILSRQMRVEQMN
                     AERINRIMATNVTGSFLCAREAVRRMSTKLGGSGGSIVNISSRAAVLGSAGEYVDYAA
                     SKAAMDALTIGLSKEVASEKIRVNSVRPGLIRTEMHASGGEPNRVDRLQSFVPMERGG
                     EPIEVAKAALWLLSDEASYTTGTFIDVSGGR"
     gene            complement(2480..2758)
                     /locus_tag="VFR42_01105"
     CDS             complement(2480..2758)
                     /locus_tag="VFR42_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367774.1"
                     /translation="MSEHELVNAAEKLSEVIENLDQSKSLLRKAYSILRPIVNDALAG
                     RLQIPGELPHRIFFFGMYEDSLPANYLSDTLFMNALGDFDEAWRQAKA"
     gene            3062..>3426
                     /locus_tag="VFR42_01110"
     CDS             3062..>3426
                     /locus_tag="VFR42_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367775.1"
                     /translation="MKNPLIVTAICGAIAGCGGGGGGAASVTTPPVVERLASYIGSYS
                     CECAGHELYSATIVLTPGTTDSVNFTTQSDYYSGANCSGAILGTLTESGTFTAKYVAT
                     VSSSVIFTTGTAAIPANVD"
BASE COUNT          746 a          961 c          985 g          734 t
ORIGIN      
        1 tcttcggtct gctggcaagc ggcgccatcc acccgcgtgt tgcgggccgg atctccttcg
       61 atgaggtcgc gacggcgcac cgtcgcctcg aagaaggtgg ccttgagggc aaactcgtgc
      121 tgtgcccgga ccttcccgcc atccactagc aactgtcacc acatgcatgg agttccatca
      181 tgcaagccgt ggatagggtg aaagccggct ctcgcaaccg ccccgttgcc gtcgcgctgc
      241 agggaggcgg cgcacacggc gcattcacat ggggcgttct cgatcgtttg ttggaagagt
      301 cggacttcga gatcgtcggc atcagtggca ccagcgctgg cgcaatgaat gccggcattc
      361 tggcggacgg gctgcgacgt ggcgggccgc cggaagcccg ggccgcactg gctcgttatt
      421 gggaagacgt cggccgcctg cccggctatg ccagcatcgc tccgacgccg agcctcgcaa
      481 ccgaacgcga atggcatctg gaccgtaatc cgttttttct atgggccgac atgctcgcac
      541 gcgtttggtc cccctatcaa atcaatccct tcaactacaa cccgttgcgt ggcttgcttg
      601 agcggatcga tttcgacggc ctgcgttgcg acgccgatgc tgcccgcgtg ttcatttgcg
      661 cgaccaatgt ccgcagcggc ctgcgacgcg tattcgacaa cagcgagttg tctgtcgatg
      721 ttctgcttgc gtcggcgtgc ctgccgctga tgaatcaggc tgtggagatc ggcggccagc
      781 actactggga tggtggctat acggggaatc cagcgttggc gccgctctac ctgaagaccg
      841 cggcggacga tgtgattatc atcggcatca atccactctt gcgcgcagaa gtgcccagaa
      901 cggcgcgcga aatcattagc cggatcgacg agatcagctt caactcgagc ttcttcatgg
      961 aactcggcgc catcgccttc gtggacgaac tcctcaaagc cggagcgccg cagaatttcg
     1021 tcaagctcct gttcgtccac cagatttgca atgagcaact cggcagcctt ggcgcctcca
     1081 gcaagatgaa caacgaccct gcctttctga ggcatttaca cacgatcggc cggcaaagcg
     1141 ccgaggactg gctcgcagaa caccgcgaag cggttggaaa agagtcgacc gtcgatctaa
     1201 cagggctcct gccgttgcaa gatggcgcct tcgccgatcc gatgggaatc aggcaaacgg
     1261 ctgcgcatac cgtaagttga cgcgatcaac tcccgttcgg cagcgagtaa gtccgttagc
     1321 gtcgacgacc ttccaaaagc agaaccagag aagcaggccc ggttggggaa ctccgaagcc
     1381 gagcaattga atgagcgtcc caggaagacg ctaaactatg aaacgccggc tcggcgattt
     1441 ccttcttcca acttactgaa accgaaatga tcgcatgatg gaaaaaataa tcctcatcac
     1501 tggcggcggg cgcggcatcg gcgcagcttg cgcactgctc gcggcccaac agggatataa
     1561 ggtctgcgtc aattacagaa gcgatggcgc cgcagcgttc aacgttgtcg ccgccattga
     1621 ggacgtgggc ggtaccgcgc tggcgataca ggccgacgtc tccgacgagg ccgccgttga
     1681 acgaatgttc gcgttggtgg atgagcggtt tggcagactt acagcactca tcaacaatgc
     1741 cggtatcttg tcgcggcaaa tgcgtgtcga gcaaatgaat gccgaacgga tcaatcgcat
     1801 catggcgacc aatgtcactg gcagcttctt gtgcgcgcgc gaggcggtgc gccgcatgtc
     1861 aaccaagctt ggcggctcgg gcggcagcat cgtcaatatc tcatcacgcg ccgctgtatt
     1921 gggttccgcc ggcgagtatg tcgactacgc tgcatccaaa gccgcaatgg atgcgctaac
     1981 gataggcctg tccaaagaag tcgctagtga aaaaattcgc gtcaattccg tccgacctgg
     2041 cctgattcgt acagagatgc acgcgagcgg cggcgaacca aaccgcgtcg accgtttgca
     2101 gtctttcgtt cccatggagc gaggtggcga gcctatcgaa gtggcgaaag cagcgctgtg
     2161 gctgctctca gatgaggctt cctacactac ggggacattt atcgatgtgt ctggtggacg
     2221 ctgacagatg aatctgcagc cgaacgctgc tgaaaccaaa tgcgtacgcg cgccgtggcg
     2281 catgccagtg ttagcttgca tggcactgaa gcagtattaa gttcgccgtg gtgcccactt
     2341 gtacgtaaca atgaacttgt gacgtaatga gggcactgac cctggcttcc caaacgatga
     2401 gcttgtgtag taatgggggc actgaccccg gctctgcgac gcggagctcg gctccgatgc
     2461 ggttcaccgg cagggacaat cacgccttcg cttgccgcca ggcttcgtcg aaatccccaa
     2521 gagcgttcat gaaaagcgta tcgctaaggt agttggccgg aagcgagtct tcgtacatcc
     2581 caaagaagaa aatccggtgc ggaagttctc cggggatttg aagtctaccc gcaagggcgt
     2641 catttacaat tgggcgcaag attgagtatg cctttcgaag aagtgatttc gattggtcga
     2701 gattttcaat tacttcgctc aacttctctg ctgcgttcac gagttcatgt tcggacataa
     2761 ttcttgcctt agccgtgagc gggttgatcg agtgctgggg ttcatagaga tccgtgtgcc
     2821 cccattccga cacatactca gcaattacgc attgcaacgt cgtttagcaa tgacgccaat
     2881 gtgctttgtt gatagtgtgg cccggatata gctaacaatg agcttgcgtt gtaatggcgg
     2941 cacggcccct gggacccacc cggcacgcgg aagccgatat ttgtgataat tgaatagtat
     3001 ttacgaggct acccgcgagt acaatgcacc attaatcata acaataattg gatgctgaaa
     3061 aatgaaaaac cctttaatag tcactgcaat ctgtggcgct atcgctggat gtggcggcgg
     3121 cggaggcggc gcggcctcgg ttaccacgcc accggttgtg gaacggttgg cttcttatat
     3181 cggatcttat tcctgtgaat gcgcaggcca cgagctttat tcggcgacaa tcgtcctcac
     3241 accgggtaca accgattccg taaatttcac aacccagtct gattactatt cgggagcaaa
     3301 ctgttcgggt gctattcttg gaacgttaac ggaaagcggc acctttaccg ctaaatacgt
     3361 tgcaacggta agctcatcag tgattttcac aaccggaacg gcagcgattc cggcaaacgt
     3421 tgatca
//
LOCUS       DASTPM010000062         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_780914, whole genome shotgun sequence.
ACCESSION   DASTPM010000062 DASTPM010000000
VERSION     DASTPM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780914"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..2282)
                     /locus_tag="VFR42_01115"
     CDS             complement(75..2282)
                     /locus_tag="VFR42_01115"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEU0367776.1"
                     /translation="MKAQERLRFLQDRWDDQVAGSLDAAELLRYRSNLLGSDLRITNF
                     GGGNTSSKISDADPLDGSRKSVLWIKGSGGDIGSIQRQGFATLYLEKLHALKGRYRGV
                     QHEDEMVEMYPLCAFGNNTIAASIDTPLHGFLPHPHVDHLHPDWGIALAAAANGARKM
                     EEFNRQFGHRLIWLPWQRPGFELAMMLEKAVDANPGCDGIVLGGHGLFTWGETPRDCY
                     RNTLAVIDQLGQFVTDHVERNGRKLFGGAVHATVANHRELALDIFPFVRGRISTRQRM
                     IGHFNDLGEVQRFVNSAEAEKLAHLGTSCPDHFIRTKIRPLFVNWEPSTNLSGLKERI
                     DAALLRYREEYRLYYESFAGSDSPKMRDASPTVVLIPGIGMFSFGKNKPEARITGEFY
                     TNAIHVMEGATSLGDGAVTGELPQAGRAAKTEAFTVHANYVALPPSEAFRIEYWQLEE
                     AKIRRQPPEKELSRRVALIVGGGNGVGRETALLAAQRGAHVVVADRDQVAAKTVAAEA
                     QDSAGKEAATFSAIDIRERESIRKSLRDAVSAYGGVDIVVNTAAIFPSSPDGVINDSL
                     WSTTLDINVTANHRLAEESAKLLHEQDLDASIVLTSSANAVVPKRGSEAYDVSKAAVN
                     HLVREFAISLAPKIRVNGISPATVVKGSTMFPRDRVIASLSKYKIAFDPAAPDEQLRS
                     QLAEFYAQRTLTHQPIDPQDCAAAILFLAGPQSRCTTGHLIPVDGGLTEAFLR"
BASE COUNT          469 a          775 c          626 g          513 t
ORIGIN      
        1 ttgaccgatc catcgcctgt cccaggcacc gtcactgact agcgcgccgc agcggcagcc
       61 ggcgccgcga agcgctaacg aagaaatgcc tcagtcaatc cgccatccac cggaatcaaa
      121 tgccccgtgg tacagcgcga ttgcggccct gccagaaaca ggatcgctgc ggcgcaatcc
      181 tgaggatcga tcggctgatg ggtcagcgtc cgttgcgcgt agaactccgc gagctgcgag
      241 cggagttgtt catccggagc agccgggtca aacgcaatct tgtatttcga caatgaagcg
      301 attacccggt cgcggggaaa catcgtcgac ccttttacca ctgtcgcggg actgattccg
      361 ttcacccgga tttttggcgc aagcgaaatc gcaaattcgc gcaccaggtg gttcaccgcg
      421 gctttgctta cgtcataagc ctcgctccca cgtttgggca ccaccgcatt cgctgaactg
      481 gtcagcacaa tgctcgcatc caggtcttgc tcgtgtaaca acttcgcgct ctcctccgct
      541 aggcgatggt tggcagtcac attgatatcc aatgtggtgc tccacaggct gtcgttgatc
      601 acgccatccg gcgaggaagg aaaaatcgcg gccgtattca cgacaatgtc tacgccgccg
      661 tacgctgaca cggcgtcgcg cagagatttg cggatcgatt cgcgttctcg aatatcgatt
      721 gccgaaaaag ttgccgcttc cttgccggcg ctgtcctgcg cctccgcggc gacggtcttg
      781 gctgccacct ggtcgcggtc ggccaccaca acatgcgcgc cgcgctgcgc cgccagcagc
      841 gccgtctccc gtccaacgcc gtttcctccc ccgacaatca acgccactcg ccggctcagt
      901 tccttttccg gaggctgccg ccggatcttc gcctcttcca actgccaata ttcaatccga
      961 aacgcttccg acggtggcag cgccacgtaa ttcgcatgca ccgtaaacgc ctcggtcttc
     1021 gccgcccggc ccgcttgcgg aagctctcct gtcacggcac cgtcacccag tgacgtcgcg
     1081 ccctccatca catgaatcgc attcgtgtaa aactcgcccg taatccgcgc ttcgggcttg
     1141 ttttttccga agctgaacat cccgatgccg gggatcaaca ccaccgtcgg gcttgcgtcg
     1201 cgcattttcg gtgaatcgct gccggcaaac gattcgtaat acagccggta ttcttcccga
     1261 tagcgcagta gcgccgcgtc gatccgctcc ttcaatccgc tcaggttcgt cgaaggttcc
     1321 caatttacga atagcggccg gatcttcgtc cgaatgaaat ggtccgggca gctggttcca
     1381 agatgtgcca gtttttccgc ttccgccgaa ttcacaaaac gttgcacttc ccccagatcg
     1441 ttgaagtgcc cgatcatgcg ttgtctggtc gaaatccgtc cgcgcacgaa cggaaaaata
     1501 tccagtgcga gctcgcggtg atttgcgacg gtggcgtgaa cagcgccgcc aaaaagtttc
     1561 ctgccgtttc gttcgacatg atccgtcacg aactggccta gttgatcgat cacggccagt
     1621 gtattgcggt aacaatcgcg cggcgtctct ccccacgtga aaaggccatg tcctcccagc
     1681 acaattccgt cgcagccggg atttgcgtcg accgctttct ccagcatcat cgccagttcg
     1741 aatccgggac gctgccacgg cagccagatc aatcggtggc caaactggcg gttgaactct
     1801 tccatcttgc gcgcaccgtt cgccgccgcc gccagcgcaa ttccccagtc gggatgcaga
     1861 tggtcgacgt gcgggtgcgg aagaaaaccg tgcaggggag tatcgatgga ggcggcaatt
     1921 gtgttatttc cgaatgcgca cagcggatac atctccacca tctcgtcttc gtgctggact
     1981 ccgcggtagc gccctttcag cgcgtgcaat ttctccagat ataaggtcgc gaatccttgc
     2041 cgctggatgc tcccgatgtc cccaccgctc cctttgatcc acaacacgct tttcctcgaa
     2101 ccgtccagcg gatcggcgtc gctaatcttc gaactggtgt ttccaccgcc gaaattcgtg
     2161 atgcgcaggt ctgaacccag caggttcgag cggtaacgca acagttcggc ggcatccagc
     2221 gaaccggcaa cctggtcgtc ccagcgatcc tgcagaaagc gaagtcgttc ctgagccttc
     2281 atatccccgg acttccctaa gcgatgaaga ggttagtcta taagctggac atcacgcccg
     2341 cttcccaacc ttacgcaatc agttaccgca gtgcaataga tta
//
LOCUS       DASTPM010000063         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1126670, whole genome shotgun sequence.
ACCESSION   DASTPM010000063 DASTPM010000000
VERSION     DASTPM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126670"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="VFR42_01120"
     CDS             <1..1846
                     /locus_tag="VFR42_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEU0367777.1"
                     /translation="VRLNQVGYPSNASKRAYLMTSTTETGATFRVLNSSGTSIFNAGI
                     GAKLGSWGSFSNVYALDFDAVTTPGTYTISVTGPASATSPSFLIDVAANLYATPLANS
                     LYFYLNERDGANFVATPLRTAGAHLNDSAANVYLTPNVNKNGRFSGDLTPTGAQIDAS
                     GGWWDAGDYLKFVQTESYTVALMLIGVRDFPNQMGAGAPVATNFTGEAKFGTDWLQKM
                     WTDTSKTFYYQVGIGDGNAQTVSDHDIWRLPQDDDTFQSCTAQYRYICRRPVFINTAG
                     GAGASISPNLAGRMAAALAVCYQIFKSTQPAYANQCLVSAEHIFDLANTAPSGNLLTV
                     IPFSFYPESEWRDDLELGATELYFALESGGLPAGLPHSDPSFYLTAAAKWANAYITGP
                     GDAGDTLNLYDVSGLAHFELYRAMALAGNPGGLATTQAALLADLKKQLDKAVAQAATD
                     PFGFGFPWGTYDTTSHGAGLAVMANEYDNLTNSTAYAAYANRWLGNILGSNAWGTSLI
                     VGDGSTFPHCMQHQVANLVGSLNGTEPLLRGAAVEGPNGIATKGALSGMISCPTNGVD
                     VFAQFNGNGAVFKDNVQSYSTVEPAVDLTASSFLAFSWTIAGAPTGTP"
     gene            complement(1880..2164)
                     /locus_tag="VFR42_01125"
     CDS             complement(1880..2164)
                     /locus_tag="VFR42_01125"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0367778.1"
                     /translation="MAVTIEIPTAFRRFTDGTPKFDCNAATIAEALDILTTQFPGLSR
                     HVRDDQGQIRQFLNVYLNEEDIRFLGGEECAVKEGDRVLLVPSIAGGSAL"
     gene            complement(2255..3517)
                     /gene="thrC"
                     /locus_tag="VFR42_01130"
     CDS             complement(2255..3517)
                     /gene="thrC"
                     /locus_tag="VFR42_01130"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU0367779.1"
                     /translation="MPEIYQLRCRECGRLWGNAPRSFCEECFSPLEVAFDYEALKSVA
                     AKEKLSKRDFNMWRYAEFLPLPEGFTFPNAVGGTPLVTSKHLAKKWGLRNLYFKNDAV
                     CFPSLSFKDRVVATALAAAGRFGFKTVGCSSTGNLANAVAAQSAQQGFEACVFIPADL
                     EPAKILNTAVYGARIVRINGNYDHVNRLCAQIADRFQWGLVNVNLRPYYAEGSKTHGF
                     EIAEQLGWKLPDNVVVPMAGGSLITKIAKAFRELVTLGWVEEKPVKFFGAQATGCSPI
                     STAVKRGLARFEPQKPDTIARSLAIGNPADGPFAIKQIRESGGWADDTSDREVVEAIQ
                     VLAETEGIFAETAGGVTAGVTQKLIREGRIRSDETTVVCITGNGLKTTDAIAAEYPAT
                     EAIAAKLEAFEAYLDAQLAASTAVAGKA"
BASE COUNT          879 a         1275 c         1080 g          829 t
ORIGIN      
        1 cgttcgactg aatcaggtcg gctatcccag caacgcgagc aaacgagctt acctgatgac
       61 atctacaacg gaaaccggcg ccacctttcg agtactgaat tcttcgggga cgtccatctt
      121 caatgcgggc atcggtgcaa aactcggctc gtggggaagc ttctcgaatg tatatgcgct
      181 cgatttcgat gcggtgacta cgcccggaac ctacaccatc agcgtgactg gcccggcgtc
      241 cgccacttcg ccgagttttc tcatcgatgt tgcagcgaac ctgtacgcta cgccgctggc
      301 caacagcctc tacttctatc taaacgagcg cgacggcgct aactttgtgg ccacgcctct
      361 acgcactgcc ggcgcgcatc tcaatgactc cgcggcaaac gtgtacctca cgcccaacgt
      421 gaacaagaat ggccgctttt cgggcgatct cactcccacg ggagcgcaaa tcgacgcgtc
      481 cggaggctgg tgggatgccg gcgactatct gaaatttgtg caaacggaga gttataccgt
      541 ggcgctcatg ctgattggtg tgcgcgattt tcccaatcag atgggcgcgg gcgcgcccgt
      601 agcgacgaac ttcacgggcg aagccaaatt cggaaccgac tggctgcaaa aaatgtggac
      661 cgacacttcc aagaccttct actaccaggt tggcatcggc gacggaaatg cccagacggt
      721 cagcgaccat gacatctggc gcttgccgca ggacgatgac acgttccaga gctgcacggc
      781 gcaataccgc tacatttgtc gtcgcccggt ctttatcaac accgccggag gcgcgggggc
      841 gtccattagc ccgaacctgg ccggtcgcat ggctgcagcg ttggcagtgt gctatcagat
      901 tttcaagtcg acgcaaccag catacgccaa ccaatgtctg gtttccgcgg aacacatttt
      961 cgatcttgcc aacaccgcac ccagcggcaa cctgctcact gtgattccat tcagctttta
     1021 tccggaaagc gaatggcgcg acgatctgga actcggcgcc accgagcttt acttcgcgct
     1081 cgagtctggc ggcctgcctg caggcctccc gcacagcgat ccctcttttt atctgaccgc
     1141 tgcggccaag tgggccaacg cctacatcac cggacccggc gacgccggcg acacgttgaa
     1201 tctttacgac gtcagcggac tcgcgcactt cgagttgtac cgagcgatgg cgctcgccgg
     1261 aaatcccggc gggctggcca cgacgcaggc agcgttgctg gccgatctga aaaaacagct
     1321 cgacaaagcc gtcgcgcagg cggccacgga tcctttcggg tttggattcc cgtgggggac
     1381 ttacgacact acttcacacg gcgccggtct ggcggttatg gcgaatgaat acgacaacct
     1441 gacgaactcc accgcgtacg ccgcctacgc caaccgctgg ctcggcaata ttctgggcag
     1501 caacgcctgg ggaacttcac tcattgtcgg tgacggcagt acattcccgc attgcatgca
     1561 gcaccaagtt gcgaatcttg tcggctcgtt gaacggcacg gaaccgttgc ttcgcggtgc
     1621 ggctgtcgaa gggccaaacg gaatcgctac aaaaggagcg ctgagcggaa tgatctcttg
     1681 cccaacgaac ggtgtcgacg tgttcgctca attcaatggc aacggagccg tttttaagga
     1741 caacgtgcag tcttactcga ccgtcgaacc ggccgtggac ctgaccgctt cttcattcct
     1801 ggcgttctca tggactatcg caggagcgcc aaccggaacg ccttaagaac agaggtgagg
     1861 cgaagtaacg aattctgcgc tagagcgctg agccgccggc gatcgagggc accagcaaga
     1921 ctctgtcgcc ttccttcacg gcgcactcct cgccgccgag aaagcgaata tcttcttcgt
     1981 tgagatagac gttcaggaat tgccggatct gtccctgatc gtcgcgcacg tgtcgcgaca
     2041 ggccggggaa ctgcgtggtc aagatatcga gcgcctcagc aattgtagcg gcgttacagt
     2101 cgaacttcgg cgttccatcc gtgaaccggc gaaacgcagt aggaatttca atcgtcacag
     2161 ccatcatcct ctccgtaggt aaaatctgtt cccagactcg atcgagcttc tacttcgtga
     2221 ttccgagcgc agcggggaat ctctcctcgg tgcgctacgc cttgcctgcc actgccgtgc
     2281 tcgctgccag ctgcgcatcg agatacgcct caaacgcttc cagctttgca gcaatcgctt
     2341 ctgtcgccgg atactcggcc gcaatcgcat cagtggtctt caagccgttg ccggtaatgc
     2401 agaccacagt cgtttcgtcg gaacgaattc gcccttcgcg aatcagtttt tgcgtaacgc
     2461 cggcagtcac gccaccggcc gtctccgcaa aaattccttc cgtctccgcc agcacctgaa
     2521 tggcttccac cacctcacgg tccgaagtat cgtccgccca gccacctgac tcacgaatct
     2581 gtttgatggc gaacggccca tccgccggat tgccgatcgc cagcgagcgc gcaatggtgt
     2641 ccggcttctg gggctcgaaa cgcgcaagac cgcgtttcac ggccgtcgaa atcggcgagc
     2701 aaccggtggc ctgcgcgcca aaaaacttca ccggcttttc ttccacccag ccaagcgtaa
     2761 ccagttctcg aaacgcctta gcgatcttgg tgatcagcga accgcccgcc atgggcacca
     2821 ccacgttgtc cggcagcttc catcccaact gttcggcaat ttcaaatccg tgcgtcttcg
     2881 agccttccgc gtagtacggc cgcaagttca cgttgaccag tccccactgg aagcggtcgg
     2941 caatctgcgc gcacagccgg ttcacgtggt cgtagttccc gttgatgcgc acaatccgtg
     3001 cgccgtacac cgcggtgttc agaatcttcg ccggctccag atccgcaggg ataaagacgc
     3061 aagcttcaaa gccctgctgc gccgattgcg ccgccaccgc gttagccagg ttgccggtcg
     3121 aagaacaacc aaccgtcttg aacccaaatc gcccagccgc cgccaaagct gtcgccacca
     3181 cacggtcttt gaaagataga gaagggaagc acaccgcatc gttcttgaaa tacaaattcc
     3241 gcaagcccca tttttttgcc agatgcttcg aagtcacgag aggagtccca ccaacggcat
     3301 tcggaaaggt aaaaccctcc ggcaacggca gaaattcggc ataacgccac atgttaaagt
     3361 cgcgcttgct cagcttttcc ttcgcagcga cggatttcag tgcttcgtaa tcgaaggcaa
     3421 cttcgagagg ggagaagcat tcttcgcaga acgaccgcgg cgcattaccc cacagccttc
     3481 cgcactcccg acaacgtaat tgataaatct ccggcatagc gatatccaat cgtggcccaa
     3541 acagcacgag ccaacggaaa tggccgaagc ggcgatacga aacatgaatg agtcagggcc
     3601 agcgcggagg ggagcaaaat aaaaatccct ccgtgccaaa gagtgccgga gggtctcaga
     3661 atctttcgag aaactctacc agctctttag cagtttttta cgtggagcaa gtcgccctaa
     3721 atcgccacgt ttcgccgcag caagcggcag acttaattaa tgacagggaa gcactccgtt
     3781 gtcaatagct tccgtacgcg ccgcataccg gaaatgattc tggaccttgc agacggctga
     3841 cctaaaactg tcctttgggg tggttccccc gctcgtcttg ccctgctatt acagcaacag
     3901 ccgattttgt ctgcgttctc tgcggtcaag tcaaagacgt tcctcaggag gggccccatg
     3961 atccgtaagc tgggactcgc ttccgtatct ttcgcagcta tcctggcctg cttcgcgcct
     4021 gacactgccg cgcaatccgg cgatggggat ctctccagac ccc
//
LOCUS       DASTPM010000064         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_838523, whole genome shotgun sequence.
ACCESSION   DASTPM010000064 DASTPM010000000
VERSION     DASTPM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838523"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            502..660
                     /locus_tag="VFR42_01135"
     CDS             502..660
                     /locus_tag="VFR42_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367780.1"
                     /translation="MRDDEALPDIVTREFHHNYLHLACSGKPPKPSVELGGGTKNQGR
                     PAWSALRS"
     gene            complement(766..1872)
                     /locus_tag="VFR42_01140"
     CDS             complement(766..1872)
                     /locus_tag="VFR42_01140"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEU0367781.1"
                     /translation="MKQTVLVILLLFACNDLAAQQQNPFDAKSAERFANLALACVHKE
                     YPNKLSHSLNSDADVAPPHKLTPAFYGCFDWHSSVHGHWLLARLVRTFPEANFVPAAR
                     EALRQSLTPQNIAQEAAYLRAEGRASFERPYGLAWLLQLVAELREWDDPQAREMAANL
                     QPLEQAAIERVSNWLPKLSNPIRIGEHNQTAFAMGLMLEYARQAHNEKFAELIASKAR
                     QFYLKDRACPLAYEPSGEDFLSPCLGEANLMRRVLPQGEFAKWLGTFLPQIPATSKSS
                     WLTPVVSPDPSDPKLAHLDGLNLSRAWMLEGIASALARNDKRLAQLRIAADAHKLAGL
                     AAVTGEHYEGGHWLGSFAVYLVTQRGLSRQPPPY"
BASE COUNT          445 a          601 c          665 g          471 t
ORIGIN      
        1 gaaggttctt ccacgcgacc ttcccaaccc gttgtggtct cttggtgtct catgctggtg
       61 tgcctgccgg caagcgcggg acatttcacg gtagccgtag cttcgggagt tcaggatgct
      121 acggtcctag gcatggcgta ctgccaacca cgggagatag ggagacctga ggttgcaggc
      181 tgggaaggtg caacttcccg ccggcgcgaa ttcgtcgctg cggatttttc gttccggtga
      241 aaggacggtt gtggagctgc gagggttctt ttgacctact gggagaacgc ttccggcgac
      301 aaactgacgt tttgtgccgg tgatatccga tttgttccac gcgacaatga agaacggaac
      361 ttcggtcgga ttagtttgct ttcgagttgt gcagcgtttg ccggcgtttc gcagggggcg
      421 cctgggattg gttggcagcg gagcggccag agccactggg cgaccgcgga ggtcggcgcg
      481 gagtacaaga acacggaggg aatgcgagac gatgaggctc tgccggacat tgtgacccgg
      541 gaatttcatc acaattattt gcaccttgcc tgtagcggga agccgcctaa gccgagcgtg
      601 gagcttggcg gtggcacgaa aaatcaaggg cgcccggcgt ggtcggcgct acgatcgtga
      661 cgccgatcga agctctggag agtccggatc ggccgtaaag ctgccaccag ggcgccgctt
      721 gtgagacagt cccaacaaat tgccgcctag gactagcgcg tcgccttagt atggcggagg
      781 ctggcgggag agaccgcgct gcgtgaccag atacaccgcg aaacttccta accagtgccc
      841 gccttcgtag tgttcaccgg taaccgccgc aagaccggcg agtttatgcg cgtccgcagc
      901 tatgcgaagc tgcgccagcc gtttatcatt cctcgcgagc gcggacgcga ttccttccag
      961 catccacgcg cggctcagat tgaggccgtc gaggtgcgcc agctttggat cgcttggatc
     1021 gggtgaaacc accggtgtca accaactgga cttgcttgtg gcaggaattt gcggaagaaa
     1081 tgtgccaagc catttcgcaa attcaccttg cggcaacacg cggcgcatca aattcgcttc
     1141 gcccagacac ggcgaaagaa aatcttcacc agaaggctcg tacgctaaag ggcacgccct
     1201 gtccttcaaa taaaattggc gcgctttgga cgcaatcagc tcggcaaact tttcgttgtg
     1261 ggcttggcgc gcgtattcta gcatcaggcc catggcaaac gcggtctggt tgtgctcgcc
     1321 gatgcggatg ggattcgaga gtttaggcag ccagttgctt acgcgctcga tggcagcttg
     1381 ttcgagcggc tgaaggtttg cggccatttc cctggcttgg gggtcgtccc actcccgaag
     1441 ttccgctacc agttgtaata gccacgccag cccatacgga cgttcaaaac tggcgcgccc
     1501 ttccgcccgc agataggccg cttcttgcgc gatattttgc ggggttaaac tttgccgcaa
     1561 ggcctcacgg gctgcaggca caaagttcgc ctccggaaaa gtccggacca gccgcgccag
     1621 cagccagtgg ccatgcaccg aagaatgcca gtcaaagcag ccgtaaaacg ccggagtgag
     1681 tttatgcggc ggagctacgt cggcatcgct gttcagggag tggctcagct tgttcgggta
     1741 ttccttgtgc acacaagcta gcgccaggtt ggcgaaccgt tcggcggatt ttgcgtcaaa
     1801 tggattttgc tgctgcgcag ccaaatcgtt gcaggcaaat aataaaagaa ttactaaaac
     1861 cgtctgcttc atgaggcgct actgtatccg aaactccgcc gcgccgcgag gcattgccta
     1921 aaaacgcgct gagcaagacc ggacggtctg gtaagatttc gcacatggcg aagagtgttc
     1981 cggtcacgtt tccggtggag agcgccggcg aagtctctgc attacttctt cgtcctgaca
     2041 acccacggtg gttgctaatt ttcgctcatg gtgccggagc aggaatgcgc cattcgttca
     2101 tggagagcct ctcgagcgaa ctcgctgcgt cgcaaattgc cacttttcgc tatcaatttc
     2161 cttatctgga aaaacgcagc gg
//
LOCUS       DASTPM010000065         4669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_290717, whole genome shotgun sequence.
ACCESSION   DASTPM010000065 DASTPM010000000
VERSION     DASTPM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290717"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFR42_01145"
     CDS             complement(<1..289)
                     /locus_tag="VFR42_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEU0367782.1"
                     /translation="MTLPKKLYLASSNPGKLNEFLAIANVAAAGLTLELLPSFAQLPP
                     YPEESPTFAENAVGKALHAANIADAAVCADDSGLVVPSLGGEPGVRSARYAG"
     gene            complement(322..1059)
                     /gene="rph"
                     /locus_tag="VFR42_01150"
     CDS             complement(322..1059)
                     /gene="rph"
                     /locus_tag="VFR42_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU0367783.1"
                     /translation="MRADGRGVDQIRPLKITPDFISTAEGSVLIELGKTRVICTATVD
                     DGVPGFLKGAGKGWVTSEYGMLPRATEERTAREAARGKQSGRTQEIQRLIGRSLRAIT
                     DQEALGERTVWLDCDVIEADGGTRTASITGAFVAFALAMERLVAAGILKSSPLIDTVA
                     ATSVGIVDDRALLDLAYEEDSRAEVDMNVVMTGDGHFVEIQATAEGRPFSGSEMQDLL
                     ALAAAGIRRLSEAQRAILPAKFMARGR"
     gene            complement(1141..1845)
                     /locus_tag="VFR42_01155"
     CDS             complement(1141..1845)
                     /locus_tag="VFR42_01155"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEU0367784.1"
                     /translation="MNGRWRIWVLIGLGVAVVIAAIYFPMLKRRVRTAAKIPQPSEEQ
                     ARRELTTPTPKNTSDPVVKAKMFWASDEDESALSPITIELALASDPVLRAKQVLNTLL
                     AGPVSSDLRTLPPDAALLAFYLLPDGTGIADFSEALATSTPSGIESEQLAVNSIARTL
                     EANVSQVKRIKILIHGQEVETLAGHLDLTQMFIVNTKAAQPFSLSVNVPVKPSAADGA
                     TPASQPPAAKGKEPRR"
     gene            complement(1842..2648)
                     /locus_tag="VFR42_01160"
     CDS             complement(1842..2648)
                     /locus_tag="VFR42_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0367785.1"
                     /translation="MRFPIDLFLFDRKFLAVAVCLAVGSGAPAQTSSNVQASAPQQSV
                     APIQAPPYPTVAPPPAQRTGLNIVVLDPAHGGTDPGARGAQGIRESDLALDLTFQVRR
                     ALEAQGFQVLQTRSGNENPSFDDRSAMANAQRGAVFVTLHISSTGLPGTVRVYTLPDM
                     PSRATAGGILSWDFAQAPYVTLSRKFGDAVQGQLAQRFKGSPNNVQTASVRQLRTTAA
                     PAIAVELSSVSVQDRGDLNKMMPGVADAIARGLAVFRPSYVVPGEAGAQP"
     gene            complement(2768..3193)
                     /locus_tag="VFR42_01165"
     CDS             complement(2768..3193)
                     /locus_tag="VFR42_01165"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEU0367786.1"
                     /translation="MKTHNFTKTVLTVAGLLTLGTFAPHAFAQSGQEQTTPAAATQPG
                     TKTGPGMRSHGEKLFADLNLTDDQKTQIKKIHQDAKAKADAVMGNSSLSDADKKTKVR
                     EIHKSAMMESKKVLTAEQRAQLKEKMKERHATKSQTSPS"
     gene            complement(3413..>4669)
                     /locus_tag="VFR42_01170"
     CDS             complement(3413..>4669)
                     /locus_tag="VFR42_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0367787.1"
                     /translation="GAGYGHGILILLFLPLFWSLPTAYMIGELSSALPHEGGYYAWVR
                     RGLGNFWGFQEAWLSLVASIFDMAIYPTLFVFYLKQIAPWFGEGNHGIMAGLFVVVGC
                     AGLNLAGIRVVGITSLWLFFLLSFPFALVVAMTPFKVNAFSEPHTAAAGTSLGILGGV
                     LVAMWNYMGWDNASTIAQEVERPQRTYPRAMIAAVVLVSATYVLPFGAVYFTGVPASA
                     FADDGAWAAIGGLIGGKLFGIEWLRFLVVLGGMMSAFGMFNALVMSYSRLPLAMARDG
                     MLPKVFAKTNGRNAPWVAILVCAAGWALCLGLGFQRLITLDIMLYGASLMLEFVTLVV
                     LRIREPELKREFRVPGGLPGAILLGLFPLGLLLLALLKSGSETVLGLNGLGFGALMIA
                     SGFAVYAATRRLRAANRASVDLVEPT"
BASE COUNT          945 a         1372 c         1441 g          911 t
ORIGIN      
        1 tgcccgcata gcgtgccgag cggacgccgg gttcgccgcc caacgatgga acaaccagcc
       61 ctgagtcgtc cgcacaaacg gctgcgtcgg caatgttcgc cgcatggagg gccttaccga
      121 cggcgttttc cgcgaaggtt ggagactctt ctggatacgg tgggagttgc gcgaagctcg
      181 ggagtagctc cagcgtcaag ccagccgcgg ctacgttggc gatcgccaga aattcattca
      241 gcttgccggg attggaggag gcgaggtaaa gctttttcgg aagtgtcatc ggttctgcgc
      301 aggcgtctgt ggcccgggcg gtcaacgccc gcgggccata aatttcgcgg gcaagatcgc
      361 ccgttgtgct tcggaaaggc ggcgaatgcc cgctgcggcc agtgccagca ggtcctgcat
      421 ttcacttccc gaaaatggcc ggccctcggc agtggcctga atttccacga aatggccgtc
      481 gccggtcatg acgacgttca tgtccacttc cgcgcgggaa tcttcttcgt aagcaaggtc
      541 gagtagggcc cgatcgtcca cgataccgac gctggttgcc gcgacagtat cgatgagcgg
      601 agaactcttg agaatgccgg cggcgacgag acgctccata gccagcgcga aggccacgaa
      661 cgccccggtg atggatgcag tgcgggtgcc cccatcggct tcgatgacgt cgcagtcgag
      721 ccaaacagtg cgctcgccga gagcttcctg gtcggtgatg gcgcgcaggg aacggccaat
      781 caagcgctgg atttcctggg tgcggccgga ttgttttccg cgtgccgcct cgcgcgctgt
      841 gcgttcttcg gttgcgcgcg gcaacatgcc gtattcgctg gttacccagc ctttgccggc
      901 gcctttcaaa aagccgggga cgccgtcgtc caccgtggcg gtgcagatga cccgcgtctt
      961 gcctagctcg atcaacacgc tgccttcggc tgtcgaaata aaatccggag tgattttgag
     1021 cggccggatc tggtctacgc cacgcccatc tgctcgcaca tggtcccctg tttggccggg
     1081 aggcctgttt ttcgagattc aagaaaagca gggccagtgt gacccgcgtc ggggcgagtg
     1141 tcaacgccga ggttctttgc cttttgcggc tggaggctga gaagccggtg tcgcgccgtc
     1201 ggcggcagac ggtttcaccg gcacgttcac cgaaagagag aacggttgcg ccgctttggt
     1261 gttcacgatg aacatttgcg tcaagtcgag gtgtcctgcg agcgtttcca cttcctgtcc
     1321 gtgaattaga attttgattc gcttcacctg cgatacattg gcttcgagtg tacgcgcgat
     1381 cgaattgacg gccagttgct cactctcgat tcctgatggc gttgacgtag ccagcgcttc
     1441 cgagaaatcg gcgatgccag ttccgtctgg gagcaaatag aaggccagca gcgccgcgtc
     1501 cggaggcaat gtgcgcaaat ccgaactcac cgggcccgcc aggagagtgt ttaacacctg
     1561 cttggcacgg aggacggggt ccgatgcaag cgccaactca attgttattg gggacaacgc
     1621 actttcgtct tcgtcagaag cccagaacat cttggctttg accaccgggt ccgaagtatt
     1681 ttttggcgtc ggtgtggtca gctcacgccg cgcttgctct tcggaaggct gagggatttt
     1741 tgcggcagtt cttacgcgcc gctttagcat gggaaaataa atcgcggcga tcaccaccgc
     1801 gacgcctaaa ccgattaata cccagattcg ccagcggcca ttcatggctg cgctcccgct
     1861 tcgccgggaa cgacgtacga cggccgaaag actgcgagtc cgcgggcgat ggcgtccgct
     1921 acacccggca tcattttgtt gaggtcgcct cggtcctgca cgctgacgct ggaaagctcg
     1981 acggcaatcg ctggggccgc tgtggtgcgc aattgccgca cgctggcggt ctgcacattg
     2041 ttaggcgagc ctttgaatcg ctgggcgagt tgcccttgca cggcatcgcc aaatttgcgg
     2101 cttaacgtga cgtacggagc ctgcgcaaaa tcccagctaa ggatcccgcc tgccgtcgcg
     2161 cggcttggca tgtccggcag cgtgtagacg cgcaccgtgc ccggcaatcc agttgaggaa
     2221 atatgtagcg tgacgaagac cgcgccgcgc tgcgcgttgg ccatcgcgga gcgatcgtcg
     2281 aaggaaggat tttcgttgcc gctgcgagtt tgcaacacct gaaatccctg cgcttccagt
     2341 gcccgccgaa cctggaacgt caggtcgagt gccagatcgc tttcacgaat gccctgggcg
     2401 ccgcgcgctc ccggatcggt gccgccatga gcagggtcga gcacgacgat gttcaaaccc
     2461 gtccgctgtg caggaggagg agctacggtc ggatacggcg gcgcttgaat cggagccacg
     2521 gattgctgcg gagcggacgc ttgcacattg gaggacgtct gcgccggtgc gccggatccg
     2581 acggcaaggc acacggccac ggccagaaat ttcctgtcaa acagaaaaag gtcgatcggg
     2641 aatcgcacga aaagcagaat agcaaatctt ggttgtgctt cgggattccg aacggccctt
     2701 cgccgcctaa aagggacgag cacttggcgc cgcgatcggc cgcaacgcga ccgaggcggc
     2761 gctagactta ggaaggactg gtctgggact tggtcgcgtg gcgctccttc attttttctt
     2821 tcaattgggc gcgctgttcg gcggtcagca cctttttcga ttccatcatc gcggatttgt
     2881 gaatttcgcg aactttggtt ttcttgtccg cgtcagaaag cgaactgttg cccatcacgg
     2941 catcggcctt ggcctttgcg tcctgatgga ttttcttgat ctgcgttttc tgatcgtcgg
     3001 tcaggttcag gtccgcgaac agcttctcgc cgtggctgcg catgcctggt cctgtctttg
     3061 ttcctggttg cgttgccgcg gcaggagtgg tttgctcctg acccgactgg gcgaacgcat
     3121 ggggagcaaa cgtccccagc gtgagcaacc ccgccactgt cagcacggtc ttcgtgaaat
     3181 tatgcgtctt cattggattt gcctttcctc cagcggccgg gcgctgggca cctgaatagt
     3241 gaacccagtt cggggaaaaa agtcgctcgg cgcagcaagc gcgaaaaaat tatcgcgagg
     3301 cttgggcgct aaaggacgaa gagcctgtgc cgccgcgaca agaatttcct tgccgtcgga
     3361 tggaacggaa ttgttttgtt gtgtctgagc aagcgatgct ggtcagccga ggttaggtcg
     3421 gttcgaccaa atccaccgat gcacggtttg ctgctcgcaa ccggcgcgtc gcagcgtata
     3481 cagcaaaacc cgaggcgatc atgagtgcgc cgaaccccaa gccgttcagg cccagcacgg
     3541 tttctgaccc gctctttagc aatgccagca agagaagtcc cagcggaaag agtccgagca
     3601 agatggcgcc aggcagtccg ccgggtacgc gaaattcgcg cttcagttcc ggttcgcgga
     3661 tgcgcaaaac taccagtgtg acaaattcca gcatcaagct ggcgccgtag agcatgatgt
     3721 ccagcgtgat gagacgctga aagccgaggc ccaggcacag cgcccaaccg gcggcgcaaa
     3781 cgaggatagc gacccagggc gcgttgcggc cattggtctt ggcaaaaact ttcggaagca
     3841 tcccgtcacg cgccatagcc aatggcaggc gcgagtagct catgaccagc gcgttgaaca
     3901 tgccgaaggc gctcatcatg ccgcccagca cgaccagaaa acgcagccac tcgatgccga
     3961 aaagcttgcc gccgatcagg ccgccgatgg cggcccaggc gccgtcgtcg gcaaacgcgg
     4021 aggcaggaac gccggtgaaa taaaccgctc cgaaaggcag gacgtaggtt gctgaaacca
     4081 gcaccacggc tgcgatcatg gcacgcggat atgtgcgctg cggccgctct acttcctgag
     4141 cgatagtgga agcgttgtcc cagcccatat agttccacat ggcgacgagc acgccaccga
     4201 gaatgcccaa gctggtgccg gccgccgcgg tgtgcggctc cgaaaaagca ttcactttga
     4261 aaggcgtcat ggcgacgacc agcgcgaagg ggaacgaaag tagaaagaaa agccacagcg
     4321 atgtgatgcc cactacacgg attcccgcga gattcagccc cgcgcagccg accaccacaa
     4381 aaaggccagc catgattccg tgattgcctt cgccgaacca cggcgcgatc tgcttcaggt
     4441 aaaaaacgaa cagcgtaggg tagatcgcca tatcgaaaat gctggcgacc agcgagagcc
     4501 aggcttcctg aaagccccag aaatttccca aaccgcggcg cacccaggcg tagtaacccc
     4561 cttcgtgagg cagtgcgctc gaaagttcgc caatcatgta cgcggttggg agactccaga
     4621 agagcgggag aaacagcaga atcagaatcc cgtggccgta gccggcgcc
//
LOCUS       DASTPM010000066         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_953918, whole genome shotgun sequence.
ACCESSION   DASTPM010000066 DASTPM010000000
VERSION     DASTPM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953918"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="VFR42_01175"
     CDS             <1..1170
                     /locus_tag="VFR42_01175"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU0367788.1"
                     /translation="GNTVAQFEESLRACRPGIGPITQIDTSRLRFHNAAEVRGFEPTR
                     HFTESQLNWLDRFAQLGIVAARDAIADSTLTWNDELRARTGVITGSCLGGIGTQDGHY
                     FGFYHEKKPRCSPTAVPRIMSNAVASHVAMEQKFIGATFTTSTACSSANHAIGQAFWL
                     IRHGTLDCALAGGSEAPISFGHLKAWEAMRVVASDTCRPFSKNRAGLILGEGAAMLVL
                     ETLENARARGAKIYAELAGFGMSADAYHLTSPLAEGAARAMNAALEDGHLSREQINYV
                     NAHGTGTQANDSTETRAIRQVFGSHVDKLAVSSTKSMHGHALGAAGALEGVATLLAFR
                     SNFLPPTANFLERDPACDLDVVPNKARTGEIEAALSNSFAFGGLNAVLAFRRITD"
     gene            1180..2094
                     /locus_tag="VFR42_01180"
     CDS             1180..2094
                     /locus_tag="VFR42_01180"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0367789.1"
                     /translation="MKLARYLLEYIAVRAVLALLVVVPNSLARKVAMLGSRVLFSCLP
                     RLRNIGLRNLELAFPELSIAERRRLLTQSFENFGRLIADFAQFPRATPADLAARVDTS
                     FLEAVARYRAAKAKGRGVIFVTTHLGNWEMLAFVFSAIFETVAYLARPLDNPFLERYT
                     SRARSRFGNRPINKRDSILEGLAILDAGGSLGLLADVNTLQRDGVFVPFFGHLACTTR
                     AVAMLALRTDAIIVPVCCVWDTNHYRVLVGEFVEAVRSASYAKKNIIDTTALYTTEIE
                     KFIRAYPGQWIWIHRRWKTRPSGQPSLY"
     gene            complement(2283..>2672)
                     /locus_tag="VFR42_01185"
     CDS             complement(2283..>2672)
                     /locus_tag="VFR42_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEU0367790.1"
                     /translation="HVNYDAIPGIIYTNPELAGVGITEDQAKEKGMNVRVGKFPFRAN
                     GRALANEDAEGVVKFVADAKTDKILGAHILQHAASELIAEPTSVIEFGGSSEDLGRTC
                     HSHPTLSEAVKEAALAVEKRALHIVNR"
BASE COUNT          496 a          808 c          749 g          619 t
ORIGIN      
        1 ggcaataccg tcgcgcagtt cgaagaatcg ttgcgggcct gtcgtccggg catcggaccc
       61 attacccaga tcgatacgtc gcggcttcgc tttcataatg cggccgaggt ccgagggttt
      121 gaaccgacca ggcattttac cgaatcgcag ctgaactggc tcgaccgctt cgcccagctt
      181 ggcatcgtgg ccgctcgcga cgccatcgct gattcgacgc tcacctggaa tgacgaactg
      241 cgcgcgcgga ctggcgtcat taccggctcc tgtttgggcg gcatcggcac ccaggatggg
      301 cattactttg ggttttatca cgaaaagaaa ccgcgttgtt cgcctactgc tgttccgcgg
      361 attatgagta acgcggtcgc cagccatgtc gcgatggaac aaaaattcat cggcgcaacc
      421 ttcactacct ccacggcctg ctcctccgcg aaccacgcga tcggacaggc attttggttg
      481 atccgacacg gcacgctcga ttgcgcgctt gctgggggca gtgaagcgcc aatctctttt
      541 ggacacttaa aagcttggga agcgatgcgg gtcgtggcgt cggacacctg tcgtcccttt
      601 tcgaagaatc gggctggtct catccttggt gaaggtgccg ccatgctggt tctcgaaaca
      661 ctggagaacg cacgtgcgcg cggggcaaaa atttacgcag aacttgccgg cttcggcatg
      721 tcggctgacg cgtatcatct tactagtcca ctcgctgaag gcgcggctcg cgccatgaac
      781 gccgcgctcg aggacgggca tctttctcga gaacaaatca attacgtgaa cgcgcacggg
      841 accgggacgc aagcgaatga ttccacggaa acacgcgcaa tccggcaggt gtttggatcc
      901 cacgttgaca aacttgccgt tagttcgaca aaatccatgc atggccacgc gttaggcgcg
      961 gccggagcat tagaaggagt ggcgaccctg cttgctttcc gcagtaattt cttgccgccg
     1021 acggcgaatt tcctcgaacg cgatccggcc tgtgatctcg atgttgtccc gaataaagcg
     1081 cgcaccgggg aaatcgaagc ggcgctttcg aattctttcg ctttcggcgg actgaacgct
     1141 gttctcgctt ttcgccgtat caccgattag ccgcgcacga tgaaattggc gcgttatcta
     1201 ctcgagtata tcgcggttcg tgccgttttg gccttgctcg tggtcgtgcc gaattccctc
     1261 gcgagaaaag ttgctatgct cgggagccgt gtccttttct cctgtctgcc acgcctgcgc
     1321 aacattggct tgcgcaacct cgagctggct tttcctgagc tctccatcgc tgaacggcgc
     1381 cggttgctca cccaatcctt cgaaaacttc ggccgtctca tcgccgactt cgctcagttc
     1441 ccacgggcca cgccggctga tctggcggca cgagttgaca cgtctttcct tgaagcagtg
     1501 gcacgctatc gtgccgccaa agccaaggga cgaggcgtca tctttgtcac gacgcatttg
     1561 ggtaactggg aaatgctcgc gtttgttttc tccgccattt tcgagaccgt cgcctacctc
     1621 gctcgcccgc tggataaccc ttttctcgag cgttacacct cgcgcgcccg cagccgtttc
     1681 ggcaaccgtc cgataaacaa gcgggattca attttggagg gcctcgccat cctcgatgcc
     1741 ggtggcagtc tcggccttct cgcagacgta aacacgttgc agcgcgatgg tgtttttgtt
     1801 ccatttttcg gccatcttgc ctgcaccacc cgcgccgtcg cgatgcttgc cttgcgcacc
     1861 gacgccatca ttgttccggt ttgttgcgtc tgggatacaa atcattaccg cgttcttgtc
     1921 ggtgagttcg tcgaagctgt ccgtagcgcc agctacgcca agaaaaacat catcgatacg
     1981 actgcgctct acactaccga aatcgaaaaa ttcattcgcg cgtatccagg gcaatggatt
     2041 tggatccatc gccgctggaa aacccgccca tccgggcagc cgagtttgta ttagggtaca
     2101 ctcgcgggac gtctggggaa gctgcgggct gctagccctg gaaatctcgg caggttgccc
     2161 agactatgcg gctgctgttc tccgagtagc gcacgcgtct cgcgtgttgg cgatcgcgga
     2221 cttttccaga agactgtttc ggcgaaacgc gcacgctacc gcatgcggag cgatccgcgg
     2281 cgctagcgat tcacgatatg cagcgcacgt ttctccacgg caagagccgc ttcctttacc
     2341 gcttcgctca gagttggatg cgaatggcag gtccggccga ggtcttcgct gctgccgcca
     2401 aactcgatca ctgacgttgg ctcggcgatt agctcagagg cggcgtgctg caagatgtgc
     2461 gcacccagaa tcttatcggt cttggcgtcg gcgacgaact tgaccacccc ctccgcgtct
     2521 tcattggcca aggcacggcc gttggcgcgg aatggaaatt tgccgacccg cacattcatt
     2581 cctttctctt tcgcctggtc ctcggtgatg cctacgccag ccagctccgg attggtgtaa
     2641 atgatgccgg gaatggcgtc gtagttcacg tg
//
LOCUS       DASTPM010000067         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_290757, whole genome shotgun sequence.
ACCESSION   DASTPM010000067 DASTPM010000000
VERSION     DASTPM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290757"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..2440
                     /locus_tag="VFR42_01190"
     CDS             23..2440
                     /locus_tag="VFR42_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU0367791.1"
                     /translation="MKLRAASSRNANPYGRFFAPLTFTVFATLCFFLGVPAVPAQTAE
                     APAVAKAPTKASAKTSQNQLSSPAIESKVNALLTKMTLEEKIGQLVQYSAGAATGPTS
                     GRTDDKDMIRKGQVGSLFNVTGAHATNELQHVAVDESRLHIPLIFGLDVIHGFRTTFP
                     LNLGLAATWDPDLVQKTTRIAAQEASASGVRWTFSPMVDIARDARWGRMSEGAGEDPF
                     LGSAMARAYVRGYQGEHLDAPDSIVACAKHYVGYGAAEGGRDYNSTEISEHTLREYYL
                     PPFRAAAEAGSGTFMSAFNSLNGIPTSANPFTIKQILKKEWGFQGFVVSDWTSVSEVM
                     AHGIAIDGATAARKAFLAGVDMDMQSNLYHQNMLNLVQSGKVPQAAIDDGVRRILRVK
                     FAMGLFDHPYTDESKESGAFLRPESVAVAKTAGTRSIVLLRNESVSGTPLLPLSAKVS
                     SVALIGPLADDAGNMIGSWGALGRGEDAVTLRRALSQKLGESRTRYAKGGNFLDASDS
                     DISAAVSAAQNSDVAVLALGEDAPSMTGEAASRAWIGLPGRQQELLEKVVAAGKPVVL
                     ILFSGRPLTLPWAFEHVPAVLAAWYPGVQAGNALADLLLGDAAPTGHLPLSWPRSVGQ
                     EPLYYNALNTGRPAADPDHPAEKGETKYVSRYIDEKDTPQYPFGFGLTYTTFRYGATS
                     ANAKELSMSALRRSLAARKQGGKVISVSADVTNTGSRAGETMAQLYVRLDGTSIAMPV
                     RMLKGFQSVTLGPGESRKVTFDVDAESFAFWGAENTFGVEPARVTLWVAPSSAEGEPT
                     ALNIAER"
     gene            complement(2481..>4186)
                     /locus_tag="VFR42_01195"
     CDS             complement(2481..>4186)
                     /locus_tag="VFR42_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU0367792.1"
                     /translation="ARFLRLLAASLGAAGTRLRALPLLAGFARRVTPAAPLFEELPPS
                     STGITWHHVNGRSPEYYLPETTGAGCAFLDYDNDGWMDIYLVNSGKCDFLAPNPPLGN
                     ALYKNNRDGSFTDVTEKAGVAAGGYGMGVAVGDYNGDGFPDLYVTQYGRSILYRNNGD
                     GTFTDVTEKAGVAAPGWASSAVWFDYDNDGKLDLFVCRFVEFDKSKNKFCGNEKTKER
                     YYCIPRVYTPARSWLFRNNGDGTFTDVSMESGIGRVLGKAWGVVATDINNDGWMDLFV
                     ANDTVQNFLFLNKKNGKFEEIGLQSGVAFSQDGRERSGMGVDSADFNQDGWQDLFVTN
                     VDQEMYSIYKNNRDLTFEDLAGPMGLGRTTRLMSGWGLKFFDYDNDGNLDLFIANGHP
                     DDRIEEHSSHVQYKEPLLLFHNNGKTLENVSAAAGPAFDKNFAARGLAVGDFTNTGAL
                     DVLISMNGEPPVLLKNKAAAGNHWLGVKLVGKKSNPDAVGAKLTWAAGTLKRTQMKVG
                     GGSYLSSHDPRMVLGLGASARVDSLDVHWPLPSGRVESFKDLPADRYITIVEGEGIKE
                     T"
BASE COUNT          823 a         1237 c         1253 g          873 t
ORIGIN      
        1 ataaaggcgg tttacgtcag tcatgaaact tcgcgccgca tcgtcacgca acgccaaccc
       61 ttacggccgc ttttttgcgc cgctcacctt cactgttttc gctactctct gcttctttct
      121 cggtgtgccc gccgtcccgg cccaaacggc tgaggctccg gccgtcgcga aggctcccac
      181 gaaagcttct gcaaaaactt ctcagaatca gctgagctcc cccgccatcg aaagcaaagt
      241 caacgcactc ctcacgaaga tgactctcga agaaaaaatc ggccagctcg ttcagtattc
      301 cgccggagcc gccaccggcc ccacttccgg ccgcaccgac gataaagata tgattcgcaa
      361 gggtcaggtc ggctctctct tcaacgtcac aggcgcgcac gctaccaacg aattgcagca
      421 cgtcgccgtc gacgaatccc gcttgcacat tccgctcatc ttcggcctcg acgtcattca
      481 cggtttccgc acaacctttc cgctgaacct cggcctcgca gccacctggg atccggatct
      541 ggttcaaaaa acgacgcgca ttgccgcgca ggaagcttcc gcttccggtg ttcgctggac
      601 tttttctccc atggtggaca tcgcgcgcga cgcccgctgg ggccgcatgt ccgaaggcgc
      661 cggcgaagat ccattcctcg gctcagccat ggcgcgcgct tacgtgcgcg gctatcaggg
      721 cgaacatctc gatgcgccag acagcatcgt cgcgtgcgct aaacactacg ttggctacgg
      781 cgcagcggaa ggcggccgcg attacaactc cacggaaatt tccgaacaca cgctgcgcga
      841 atattacttg ccgccgttcc gcgcagccgc ggaggctggc tccggaacat ttatgagcgc
      901 attcaattcg ctcaacggaa ttcccacttc cgccaatccg ttcaccatca aacaaattct
      961 gaaaaaagaa tggggcttcc agggattcgt ggtcagcgac tggacttccg tgagcgaagt
     1021 gatggcgcac ggaatcgcga ttgacggcgc caccgccgcg cgcaaagcat tcctcgccgg
     1081 tgtcgacatg gacatgcaga gcaatctgta tcaccagaat atgctcaatt tggtgcaatc
     1141 cggtaaagtc ccgcaagccg cgattgacga cggtgttcgc cgcatcttgc gcgtgaaatt
     1201 tgctatgggt cttttcgatc atccttacac ggatgagagc aaagaatccg gcgcgttttt
     1261 gcggccggaa agcgtcgccg ttgcgaagac cgccggcact cgttccatcg ttctgttgcg
     1321 caatgaatct gtcagcggca ctccgctgct tccgctttcc gcaaaagtct cgtcggttgc
     1381 actgattggg cctctcgccg acgacgccgg caacatgatc ggttcgtggg gcgcgctggg
     1441 acgcggcgaa gatgccgtca ccctgcgccg cgccctttcg cagaaacttg gcgagtcgcg
     1501 cactcgctac gcaaagggtg gaaattttct ggatgcctcg gatagtgaca tttccgcagc
     1561 cgtcagcgcc gcacagaact ccgatgttgc ggtcctcgcc cttggtgaag acgcgcccag
     1621 catgaccggc gaagccgcat cccgcgcatg gatcggtctg cccggccgcc agcaagagct
     1681 tcttgaaaaa gttgtggccg ccggaaaacc ggtcgtgctg attctgttca gcggccgccc
     1741 gctcactctg ccctgggcgt ttgagcatgt gccggcggtg ctcgctgcct ggtacccagg
     1801 ggtgcaagcc ggcaacgcgc tggcggactt gctgcttggc gacgctgcgc ccaccggcca
     1861 ccttccgctc agctggcccc gctcagtagg gcaggagccg ctgtactaca acgcgctcaa
     1921 caccgggcgt ccggccgccg atcccgacca tccggcggag aagggcgaaa ccaaatacgt
     1981 atcgcgctac atcgacgaga aggatacgcc gcagtatcca ttcggcttcg ggctgacgta
     2041 cacgacgttt cgctatggag cgacctcagc caatgccaag gaactttcca tgagcgcgtt
     2101 gcggcgttca ctcgctgcga gaaagcaagg tggaaaagta atttccgtga gcgcggacgt
     2161 tacgaacacc ggatcgcgcg cgggagagac gatggcgcaa ttgtatgtgc ggctcgatgg
     2221 aaccagcatc gcgatgccgg tgcgaatgct gaaaggattt cagagcgtta cgctggggcc
     2281 gggcgagtcg cgcaaggtga cgttcgatgt cgacgcggaa tcatttgcat tctggggcgc
     2341 ggagaatacg tttggtgtcg agccggcgcg tgtgacgctg tgggttgctc caagcagcgc
     2401 cgaaggcgag ccgaccgcgc tcaacatcgc cgaacgatag gcgctagtcc gggtttcgct
     2461 tttctttccg ggatcttcgg ttaagtctcc ttgatgcctt cgccttcgac gatggtgatg
     2521 taacgatcgg cggggagatc cttgaagctt tccactctgc cgctgggcag gggccagtgg
     2581 acatcgagac tgtccacgcg tgcgctggca ccgaggccta acaccatgcg cggatcgtgg
     2641 gatgagagat aactgccgcc gccaaccttc atttgcgtgc gcttgagagt tccggcggcc
     2701 caggtgagtt ttgctcccac tgcatcggga ttggattttt tcccaacgag tttcacgccg
     2761 agccagtgat tgcctgccgc ggctttattc ttcagaagaa ccggcggttc gccattcatg
     2821 gaaatcagaa catcgagagc gccggtgtta gtgaaatctc cgacggccag gccgcgcgcg
     2881 gcaaaattct tgtcgaatgc cggacctgca gcagcgctta cgttttccag agtcttgccg
     2941 ttattgtgaa agagcagcag cggctccttg tactgcacgt ggctggaatg ctcttcgatg
     3001 cggtcgtctg ggtggccgtt ggcgataaag agatcgagat tcccatcgtt gtcgtagtcg
     3061 aaaaacttca ggccccagcc gctcatcaga cgagtggtgc ggcccaaacc cattggcccc
     3121 gcaagatctt caaacgtcag gtcgcggttg ttcttgtaga tggaatacat ttcctgatcg
     3181 acgttggtga cgaagaggtc ctgccagccg tcctggttga aatcggcgga gtctactccc
     3241 atgcctgagc gttcgcggcc atcctggctg aaggcgacgc cggactgcag gccgatttcc
     3301 tcaaactttc cattcttctt gttgagaaac agaaaattct gcacggtgtc gttggcgacg
     3361 aacagatcca tccatccgtc attgttgata tcggtggcca cgacgcccca agctttgccg
     3421 agaaccctgc caatgccgga ttccatgctg acgtcggtga aagtgccgtc gccgttgttg
     3481 cggaagagcc agcttcgcgc gggagtgtaa acgcggggga tgcagtagta acgctctttg
     3541 gttttttcgt tgccgcagaa tttgttttta cttttgtcga attcgacgaa gcggcagacg
     3601 aagaggtcga gtttgccgtc attgtcgtag tcgaaccaaa cggcgctgga agcccagccg
     3661 ggcgcggcga cgccggcttt ttcggtgacg tcggtgaagg tgccgtcgcc gttgttgcga
     3721 tagagaatgc tgcggccgta ttgcgtgaca taaagatcgg ggaagccgtc gccgttgtaa
     3781 tcgccgacgg caacgcccat gccgtagcct ccggcagcga caccggcctt ttccgttacg
     3841 tctgtgaagg atccgtcgcg attgtttttg tagagcgcgt tacctaacgg aggattgggc
     3901 gcgaggaaat cgcacttgcc gctgttgacg aggtagatgt ccatccagcc gtcgttgtcg
     3961 taatcgagga aagcgcagcc ggctccggtg gtttccggca agtagtattc gggcgagcgg
     4021 ccgttgacgt ggtgccaggt gatgccggtg gaggagggcg gcagctcctc aaagagaggc
     4081 gctgcgggag tgacacgacg agcgaagccg gcaagtagcg gaagtgcgcg cagacgagtg
     4141 cctgcggcgc cgagcgatgc ggcgaggagg cgaagaaagc gggcac
//
LOCUS       DASTPM010000068         7686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_867430, whole genome shotgun sequence.
ACCESSION   DASTPM010000068 DASTPM010000000
VERSION     DASTPM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867430"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFR42_01200"
     CDS             <1..259
                     /locus_tag="VFR42_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367793.1"
                     /translation="VLPTGLPAVFELLSGGSSNMREAVVIEFAGPDKAIFSSRLPVEF
                     EDRVQIENDRGYRAKAKVIAVRYHDGKTAVAVQLVSGTFLG"
     gene            267..1706
                     /locus_tag="VFR42_01205"
     CDS             267..1706
                     /locus_tag="VFR42_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367794.1"
                     /translation="MNNTLTTLKLPGAVKGEIARQEQWEELRSVFPIYLALARHLEFE
                     IPFGPDRRNLPAKADAELNSEVQNWVNSMDQRVMVHQLRHLLQMTTLNASESGLRALI
                     LRHLRKTAKNNTDRDKIDFLLVQYFALCAPPKIYHKQIDLNDTALVMRPVLGEPDPTP
                     LAWCEPLEKMIEALRAFRSLRDMLKTNFIEQGRRVKESAGGMFYDPSALLAFIRFNFL
                     VRRTLIELMHADLIAIRAGLAQLETNGIRFLDAHQGGFSANESITRVKQAAEDWKQPF
                     QKEYTERTVNQAFGKLLGLRTDVERALDKVTARQTPERPSGNDIAPLGRASSAPARPA
                     AEAGKTPAKPESGLDFEGCMEQIWEQLIAAPPSRGRSMTTIRLGSARILMSSWEVAAF
                     VSEDGPSAEDLRRAVVARALVTAAVESARQTGNATVLDRSLSVARVEVTRLLERVDAA
                     KQAKDTEAAVNLGISAKRLVSAIDEAEQL"
     gene            1748..2431
                     /locus_tag="VFR42_01210"
     CDS             1748..2431
                     /locus_tag="VFR42_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367795.1"
                     /translation="MPTPSVLILTDDSEFARLLSARWRAERRPPSLTVLNSQVEKTSH
                     MDQHDLVIIGPVAEGILPGILSALPATAAAILCAPAESREIHQLRLQNPRLPLVPLRD
                     DWTQTLVLVAGESLRRVEAARQTRQALAYAAQSERDAILGRYMLEMKHSVNNALTSIL
                     GNAELLLLEPGQLTAQSLQQIKTVQHMSLRLNEIMQRFSSLASEIRDTETPSQPETEA
                     AAENVSPRA"
     gene            2564..2926
                     /locus_tag="VFR42_01215"
     CDS             2564..2926
                     /locus_tag="VFR42_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0367796.1"
                     /translation="MLKKVLIVDDSPAQVKLMQGFLEHEGYQPVGLNDPLRVEETISS
                     VRPSVILLDVVMPDRNGFQVCRELKGSSEFQGIPVILVTSKDSASDKYWGEQQGADAY
                     VTKPFTKDELLRAVRRFA"
     gene            2933..3460
                     /locus_tag="VFR42_01220"
     CDS             2933..3460
                     /locus_tag="VFR42_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU0367797.1"
                     /translation="MTDDFQLAPLPPDADTAEMLEAIGPLEERPAESQYCVFRSGRER
                     FCLPVLDVEEVLEWPIVTKLPLAPGYMMGIFNLRGAIVPLIDIAITEGRRPGLHPKHV
                     VVASLRADSHHEDIRLGIASDEVVGTYSVTSEDMLRQAPENVPHCVGMLRHDDRLALV
                     LDLPRLLENFPGPSI"
     gene            3522..4979
                     /locus_tag="VFR42_01225"
     CDS             3522..4979
                     /locus_tag="VFR42_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU0367798.1"
                     /translation="MKSRLSSTIWTLVIVNGLAFFVAIGLAFHAGREANGNSLLDFAS
                     LPGGASGAVFGAFVLALAVAGFVAWRLGSAVITPVHQLAEFSERLAAGDPRARADVLS
                     NDDLGYIAENLNRAVAKVSKANSNQEASESLQRSITELLAVINQVARGELALRGKVTN
                     DALGNVTDSINYMLDNFTKVLERVRKAAMEVTACSNNILVAADEMQAGATQQDQEITN
                     TSSAVEELTVSMKQVSNNAEASAEAARRALDAAEQGNRAVRDTLEGMQRIRASVQATA
                     KKIKSLGDRSLEISEIINVINDITEQTNLLALNAAIEAARAGEAGRGFAVVADEVRKL
                     AEHSRSATKDIAALIKAIQAETNEAVVVMEEGTKEVESGATLADQAGRALDAISSVVR
                     QSAELVQEISLASKQQVRGTEGVAHAMQIISNITRQTSQGTRGTVATVSQLVKLSDQL
                     NEALSQFRAAQKQSGSSASVADQHDDRQVPVAALR"
     gene            5087..5947
                     /locus_tag="VFR42_01230"
     CDS             5087..5947
                     /locus_tag="VFR42_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEU0367799.1"
                     /translation="MSLTEHELSEIRMLIEERTSIRFDESRERFFSTRVREHMARKGH
                     ARGTDLLRSIRKTNFEFQALLESLLTQETSFFRYPGVFESFEKRILPELHIKKFWKNP
                     RSLRIWSAGCSTGEEPYSIAITIADAISFSDAWNVEILATDIGRHALATAERGIYSGR
                     SIASVSERQLAVHFQPVEGGHEVRPRLRKMVSFVHMNLNNGVYVGKMDVIFCMNVLIY
                     FSEERRRDLVQHFYNALEPGGYLFLGHSESISKMPVKFQAIVLNDCILYRKPTVEEAQ
                     KAELVTEGQA"
     gene            5944..>7686
                     /locus_tag="VFR42_01235"
     CDS             5944..>7686
                     /locus_tag="VFR42_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU0367800.1"
                     /translation="MSQPLDRESVEIFLQEASEHLQYLREYIGVLQEIEPRREDLERL
                     YISAHTLSGTSASYGFPHFSEVAAKLAHVFQYATQASLGEDLHGPLTEFLSDGISLLE
                     TDILEISDTGNEVVDDVSAFKERYRFAFPADPAPLGYSSSVEQPEEPVDEQHALAQTG
                     SYFDSLPEDDDVPSEILEFFQPEAEEHLQIVSDCLLSLEANHSDEEINKLFRAIHTVK
                     GSAAQVGLKRLGGIAHRVEDLIGRLREGELEPSAQVIDLCLESVDVLKKTLHKQWPDE
                     IQMRTGVDSLLNRIAELAPAETEEAVTSAAVTTPESSDSGPRRDSKPGKTITATSAAK
                     SVRIALSRLDRMMNTVGELVINRTVMVGRVAELERLRETLSFSKERLQGKVVEFQEKY
                     EFNRIGSNRGPLSQNTSPTPQMLTSAAAGDTSFWSEFSELEMDRYDDVNILSRSMTEI
                     SADVNEVLSQLNGFIGRVEGDIDEFTKLAHHLQDEITAARMVPIGTLYSRLSRAVRDA
                     ANSTGKLVELDLSGSETELDNNIIQQISDPLVHLVRNSVAHGIELPDGRSLGGKPETG
                     RVTLRAYHRGNHIYI"
BASE COUNT         1638 a         2565 c         1968 g         1515 t
ORIGIN      
        1 tgtgttgcca actgggctgc cggctgtgtt cgagttgctt agcggtggct cgtcgaatat
       61 gcgcgaagcc gtcgtcatcg agtttgcggg ccccgacaaa gcgatcttta gctccagact
      121 gcctgtggaa tttgaggatc gggtacagat cgagaacgat cgtggctacc gcgccaaagc
      181 aaaagtaatt gcggtgcgct atcacgatgg aaagaccgct gtggcggtac agcttgtcag
      241 cggaacgttt cttggatgaa gaggccgtga acaacacact gaccactcta aagctccccg
      301 gggccgtcaa gggcgaaatc gctcgccagg aacaatggga agagctgcgc tccgtctttc
      361 ctatttacct tgccctcgct cgccatctcg aattcgaaat cccatttgga ccggaccggc
      421 gaaatcttcc ggcaaaagcc gatgccgaac tcaacagtga agtccagaac tgggtcaact
      481 ccatggacca gcgcgtcatg gttcaccagt tgcgccatct gttgcagatg accacgctca
      541 acgcgagcga aagcggcttg cgcgccctga tcctgcgtca ccttcgcaaa accgcgaaga
      601 acaatactga tcgcgacaaa atcgactttc tcctggtgca gtatttcgcg ctctgcgctc
      661 ctccgaagat ttatcacaag cagatcgact tgaacgacac cgctctggtc atgcggcccg
      721 tgctgggcga acctgatccg actccgctcg cctggtgcga gcctctcgaa aaaatgatcg
      781 aagccctgcg cgcgttccgc agcctacggg acatgctcaa aaccaacttc atcgaacagg
      841 gtcgccgcgt gaaggaatca gccggcggca tgttctacga cccttccgct ctgctggctt
      901 tcatccgctt caattttctg gttcgccgca cattgatcga gctgatgcac gccgacctca
      961 ttgccattcg cgcgggcctc gctcaactcg aaaccaacgg aattcgcttc ctggacgccc
     1021 accagggggg cttctccgcc aacgaatcca tcacgcgcgt caagcaagct gccgaagact
     1081 ggaagcagcc tttccagaag gagtacaccg aaagaacggt gaatcaggcc ttcggcaaac
     1141 tgcttggctt gcgcaccgat gtcgaacgcg cgctcgataa agtcacggct cgtcagacgc
     1201 cggagcggcc gtccggcaac gatattgcgc ccctcgggcg agcctcttcc gcccccgcac
     1261 gtccggccgc ggaagccggc aaaacgcccg caaaacctga gtcaggtctc gactttgaag
     1321 gctgcatgga acaaatctgg gagcaactga tcgctgcgcc gccgtctcgt ggccgttcca
     1381 tgaccactat tcgccttggc tccgcgcgca tcttgatgtc ttcctgggaa gttgccgcgt
     1441 ttgtaagcga ggatggccct tccgccgaag atctgcgccg cgcggtcgtc gctcgcgcgc
     1501 tcgtcaccgc cgctgtggaa tccgcgcgcc agaccggcaa cgccactgtg ctcgaccgct
     1561 ctctctcggt cgcgcgggtc gaggtcacgc gccttctcga gcgcgtcgac gccgccaagc
     1621 aagccaagga taccgaggcc gcggtcaatc tcggcatcag cgccaagcgt ctggtttccg
     1681 ctatcgatga ggccgaacag ctttaactgc ggcaatggca gcgaaagttc ttccggagca
     1741 gcgacacgtg ccaaccccta gcgtcctaat tcttaccgat gacagcgaat tcgcgcgtct
     1801 gctcagcgcg cgctggcgcg ccgaacgccg cccgccttct ctgaccgtgc tgaatagcca
     1861 ggttgagaaa acgtcgcaca tggaccagca cgatctcgtt atcatcggtc cggtagccga
     1921 aggaatcctc cctggcatac tgtctgccct tcccgccact gccgctgcca ttctctgcgc
     1981 tcccgccgaa tcccgcgaaa tccatcagct ccgtttgcaa aatccgcgcc tgcctttggt
     2041 ccccctgcgc gacgattgga cacaaaccct cgttctggtc gcgggggaat ctctgcgccg
     2101 cgtcgaggcc gctcggcaaa cccgtcaggc tctcgcatac gccgcacaaa gtgaacgcga
     2161 tgccatcctc ggccgctaca tgctcgaaat gaagcacagc gtaaacaatg ctctgacctc
     2221 gatactcggc aatgccgaac ttcttctcct cgaacccggc cagcttactg ctcagtcgct
     2281 ccagcaaatc aagaccgtgc agcacatgag tttgcgcctg aacgagatca tgcaacgttt
     2341 ctcatcactg gccagcgaaa ttcgcgacac tgagactcct tctcagcctg agacggaagc
     2401 ggccgccgaa aacgtttctc cgcgcgcctg acgggtgcgc ccgctacagc taacaccatg
     2461 atttcacgcc actttctcgc tttcgacaca tctaaccttc ccgccccaaa catggccgcc
     2521 gacgtgcacc tcctgcttcg gacaactccg gagaattctg cgcgtgctaa aaaaggtcct
     2581 catcgttgac gactcgccgg cccaagtgaa gctcatgcag ggcttccttg agcacgaagg
     2641 ctatcagcct gtcggattga acgacccgct ccgcgtagaa gagaccatca gcagcgtgcg
     2701 acccagcgtc atcttgctcg acgtggtcat gcctgaccgc aacggtttcc aggtttgccg
     2761 cgaactcaaa ggcagctccg aattccaagg cattcctgta atcctcgtca cctccaagga
     2821 ttccgccagc gacaaatatt ggggcgaaca gcaaggtgcc gacgcttacg tcaccaaacc
     2881 ttttaccaag gatgaacttt tgcgagccgt ccgcagattt gcctaaaaac tcatgactga
     2941 cgacttccaa ctcgcaccgc ttccgcccga cgctgacaca gcagaaatgc tggaagccat
     3001 tggcccgctc gaagagcgcc cggccgagtc gcaatactgc gtcttccgca gcggccgcga
     3061 acgcttttgc ctgccggtac tagacgtcga agaagttctc gaatggccca tcgtcaccaa
     3121 gcttcccctc gctcccggct acatgatggg aatctttaat ctccgcggcg cgatcgttcc
     3181 gctgattgat atcgcgatta ccgaaggtcg ccgccccggc ctgcatccca agcacgtcgt
     3241 cgtcgcatcc cttcgcgccg acagccacca cgaagacatt cgcctcggca tcgcttcgga
     3301 cgaagtcgtt ggcacctatt ccgtcacatc ggaagacatg ctgcgccagg cgccggaaaa
     3361 cgttccgcac tgcgtgggca tgttgcgcca cgatgaccgt cttgctctgg ttttggattt
     3421 accccggctt ctggagaatt ttcccgggcc gtcgatctga tttttgctag gtggcgttac
     3481 ataacgccaa cgccacccgc tgatgagttt tggaggcagt gatgaaatcg cgattgagtt
     3541 ccaccatctg gacgctggtc atcgtcaacg gtctggcgtt cttcgtcgcc atcggcctgg
     3601 cattccacgc cggccgtgaa gcgaacggca attctctact ggacttcgca tcgctcccgg
     3661 gcggcgccag cggagccgtc ttcggcgcgt tcgtgctcgc tctcgcggtc gcaggcttcg
     3721 tcgcctggcg gctgggcagc gcggtcatca caccggtgca tcagctcgcc gagttctccg
     3781 agcggcttgc cgccggcgat ccccgcgcgc gcgctgacgt tctgtccaac gacgatctcg
     3841 gctacattgc ggaaaatctg aatcgcgccg tcgccaaagt ttccaaggcc aacagcaacc
     3901 aggaagccag cgaatcgctg cagcgcagca ttaccgaact gctcgccgtc atcaaccagg
     3961 tcgctcgcgg cgaactcgcc ctgcgcggca aggtgaccaa cgacgcgctc ggcaacgtca
     4021 ccgattccat taactacatg cttgacaact tcaccaaggt tctcgaacgc gtccgcaagg
     4081 ccgctatgga agtcaccgct tgctccaata acattcttgt cgccgctgat gaaatgcagg
     4141 cgggcgccac ccaacaggat caggaaatca ccaacacctc cagcgccgtg gaagagctca
     4201 ccgtctccat gaaacaggtg tccaacaacg ccgaagccag cgccgaagct gcacgccgcg
     4261 ccctcgacgc cgccgaacag ggcaaccgcg ccgtgcgcga cacgctcgaa ggcatgcagc
     4321 gtattcgtgc ctcggtgcaa gccaccgcca agaaaatcaa atcgctcggc gatcgttctc
     4381 tcgaaatttc ggaaatcatc aacgtcatca acgacatcac cgaacagacc aatttgctcg
     4441 ctttgaacgc ggccatcgaa gctgcgcgcg ccggtgaggc tggccgcgga ttcgcggtcg
     4501 tcgccgacga agtccgcaag ctcgccgagc actctcgcag cgccaccaag gacatcgcag
     4561 ccctaatcaa agctatccag gccgagacga acgaagctgt cgtggtcatg gaagaaggca
     4621 ccaaggaagt ggagtccggc gccactctgg ccgatcaggc cggacgcgcg ctcgacgcca
     4681 tctccagcgt cgtgcgccaa tccgccgaac tcgttcagga aatttctctc gcttccaaac
     4741 agcaggtgcg cggcacggaa ggtgtcgccc acgcaatgca gatcatttcc aacatcacgc
     4801 gccagacgtc gcaaggtacg cgcggcaccg tcgccaccgt cagccagttg gtcaagcttt
     4861 ccgatcagct caacgaagcg ctctcgcaat tccgcgccgc gcaaaaacaa tccggatcca
     4921 gcgcatcggt cgcggaccaa cacgacgacc ggcaagttcc cgtcgccgcc ctccgctagg
     4981 aggtcgtttt cttaactcct cttaggaggc cggggacttt agccccgacc taagagcaag
     5041 gactaagggg ctttagcccc tgaagagact aggcaagggc ggcccagtga gtttgactga
     5101 acacgaattg agcgaaatcc gcatgctgat tgaggagcgc acaagcattc ggttcgacga
     5161 atcgcgcgag cgattcttct ccactcgtgt gcgcgagcac atggcacgca agggccatgc
     5221 ccgcggcacc gacctgcttc gctccattcg caaaaccaat tttgagtttc aagcactgct
     5281 ggaatccttg ctcacacagg aaacttcttt cttccgctat cccggtgtct ttgaaagttt
     5341 tgaaaaacgc atcctgcccg aactgcacat caaaaagttc tggaagaacc cccgctctct
     5401 ccgcatctgg agcgcgggct gttccaccgg cgaagagccc tattcgatcg ccatcaccat
     5461 cgccgacgcc atttcctttt ccgacgcctg gaacgtcgaa attctcgcca ccgacatcgg
     5521 ccgccatgcc ctcgccacgg cggaacgcgg tatctattcc ggccgcagca tcgccagcgt
     5581 tagtgaacgc cagcttgccg ttcatttcca gcccgtcgaa ggcggccacg aagttcggcc
     5641 gcgcctccgc aaaatggtca gcttcgttca catgaacctg aacaatggcg tctacgtcgg
     5701 caaaatggac gtcattttct gcatgaacgt cctcatctat ttctccgaag agcgccgccg
     5761 cgacctggtc cagcattttt acaacgcgct cgagccgggc ggctacctgt tccttggtca
     5821 ttccgaatcc atttccaaaa tgccggtcaa atttcaggcc atcgtcctca acgattgcat
     5881 tctctatcgc aagcccactg tggaagaggc tcagaaagcc gagctcgtca cggagggcca
     5941 agcgtgagcc agccgctcga tcgcgaatcc gtcgagatat tccttcagga agcttccgag
     6001 cacctgcaat acctgcggga atacatcggc gtgctgcagg aaatcgaacc gcgccgcgag
     6061 gatctggagc gcctgtacat ttccgctcac acgctcagtg gcacttccgc cagctacggc
     6121 tttccccact tttctgaagt cgccgccaag ctcgctcacg tcttccaata cgccacgcaa
     6181 gcttccctcg gcgaagatct ccatggtccc ctgaccgaat ttctctctga tggcatctcg
     6241 ctgctcgaaa ccgatattct cgaaatcagc gacactggca acgaggtcgt cgacgacgtc
     6301 tccgctttca aagagcgata tcgcttcgcg tttccagccg atcccgcgcc gctcggctat
     6361 tcctcctcag tcgaacaacc cgaggaaccg gttgatgagc agcacgctct cgcgcaaaca
     6421 ggctcctatt tcgattcgct tccggaagac gatgacgttc cttcggaaat cctggaattc
     6481 tttcagcctg aagccgaaga gcatctgcaa atcgtcagtg attgcctgct ttctttagaa
     6541 gccaatcaca gtgacgaaga aatcaacaaa cttttccgcg ccatccacac cgtaaaaggt
     6601 tccgccgccc aagtcggcct caagcgtctc ggcggtatcg cccaccgcgt cgaggatctc
     6661 attggccggc tccgcgaagg tgaactagag cccagcgccc aggttatcga tctctgcctc
     6721 gaatccgtcg acgttctgaa aaagactctt cacaagcagt ggcccgacga aattcagatg
     6781 cgcaccgggg tcgattccct gctgaatcgc atagccgaac ttgcaccggc cgaaacggag
     6841 gaagcagtaa cgtcagccgc cgtcactact cctgaatctt ccgattctgg gccgcgccgc
     6901 gattccaaac ctggcaaaac catcactgca acctccgctg ccaaatccgt tcgcatcgcg
     6961 ctctctcgtc tcgaccgcat gatgaacacc gtcggggaac tcgtcatcaa ccgcaccgtc
     7021 atggtcggac gcgtcgctga actcgagcgc ctccgcgaaa ccctcagctt ttccaaagag
     7081 cgccttcagg gcaaggtcgt cgagttccag gagaaatacg aattcaaccg catcggatcc
     7141 aatcgcggcc cgctgtcgca aaatacttct cccacgccgc aaatgcttac cagcgccgcg
     7201 gccggcgata cttccttctg gtccgagttc agcgaactcg aaatggaccg ctacgacgac
     7261 gtcaacatcc tctcccgttc catgactgaa atttcagccg acgtcaacga agtcctctct
     7321 cagctgaacg gcttcatcgg ccgcgtcgaa ggcgacatcg acgagttcac caaactcgcc
     7381 catcacctgc aagacgaaat caccgccgcg cgcatggtgc ctattggcac tctttactca
     7441 cgcctttcac gcgccgttcg cgatgccgcc aactccaccg gcaagctcgt cgaactcgac
     7501 ttatctggca gcgaaaccga actcgacaac aacatcattc agcagatttc cgatccgctg
     7561 gttcacctcg tccgcaattc cgtcgcccat ggcatcgaac ttcccgatgg ccgctcgctc
     7621 ggcggcaagc cagaaaccgg tcgcgtcact ctccgcgctt accatcgcgg caatcacatc
     7681 tacatc
//
LOCUS       DASTPM010000069         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_60155, whole genome shotgun sequence.
ACCESSION   DASTPM010000069 DASTPM010000000
VERSION     DASTPM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60155"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1511
                     /gene="glgC"
                     /locus_tag="VFR42_01240"
     CDS             258..1511
                     /gene="glgC"
                     /locus_tag="VFR42_01240"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU0367801.1"
                     /translation="MGVTTMQDMRDAIVVLLAGGQGERLWPLTRDRAKPAVPFGAVYR
                     IIDITLSNCVNSGLRRVFVLTQYKALSLSRHVRAGWSGLAGLGEYIEILPPQMRVSKE
                     WYQGTADAVYQNIYSIGSERSKHVFILSGDHIYKMNYAKMLQQHLDSGAEVTVGTIQI
                     PTKEAARQFGVIEVDKDWRIVGFEEKPAAPKESPHNPGRCNASMGIYIFNTQLLIPVL
                     LADSEDPKSHHDFGKDILPRIISKYRVFAYNFVDENSSESLYWRDVGTIDAYYEANMD
                     LVSVSPIFNLYDHYWPLRTWQHQYPPAKFVFADPDRMGAAVDSIVAGGSVISGGKVTR
                     SVVGNNVRVNSYCDVSDSILYNHVNVGRHSKIRRAIIDRHVSLPEHTEIGFDVEADKR
                     RYHVTESGIVVVVRQESLIEEPETA"
     gene            1526..2743
                     /locus_tag="VFR42_01245"
     CDS             1526..2743
                     /locus_tag="VFR42_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU0367802.1"
                     /translation="MFIATKDLILPSTTTGSFPRPRWFDVCMWGKPLDTCMLDVRFRE
                     KFQDALAVVISDQERSGLDILSHGDFHVDEDLAGRAWHHYPLQRWAGFEGDYLQPEET
                     TAPWLHYPPGTLLNEIYTGWRWPHVVDKIEHRPLGYAKIWRLAQSKTRKPVKFGTCCS
                     QVMALFLDIHTPKYKDKRQIIWDMAEAMNKELLALRDAGCRCIQIEEPTFHFMANTFG
                     KDHDEVKFMIDAFNREVQGLDDVELWIHTCWGNPNMQRVMEDTSYANSIEIYLERCRG
                     DVWTLEMKDRNQKDLELFAPFKSNLKKKIAIGVVSHRQLQADRPEEVAAEIRKALKYI
                     PAEKLIVSNDCGFGRQGCNREIAFYKASAIAQAANIVRKELGLPTTYIPAADPGLQVD
                     VVPGRAAKAGQAS"
     gene            complement(2756..>3125)
                     /locus_tag="VFR42_01250"
     CDS             complement(2756..>3125)
                     /locus_tag="VFR42_01250"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEU0367803.1"
                     /translation="LYPVLVPQVDADGNERDGVRLPELSVPLATYASWNLRDSSIGAP
                     SERVAFEISYLPFPKTAAERKNSGDPRKSIAERYIGREDYVTRYAHAVDELIKQRWIL
                     PEDRDALLHRAEQEWAEATK"
BASE COUNT          718 a          815 c          943 g          649 t
ORIGIN      
        1 cggatggcgt gatgggactt cacgggtggt gctggaattc ggaaacgggc aagctttacg
       61 cttcgacaac ctcgcgtacc agcaccctta gttagggctt tcccacagcc agcgtttggc
      121 ccagccaaga cggcaaaact aaaggcatcg aaattaacag gccgctaacc agggagacgt
      181 ggacccagat attgtattgc ggctggctgg tgcatagaat ggcgcggccc ggtagatgag
      241 tgcgagaggt ggtcgcgatg ggtgtcacga caatgcagga catgcgggat gcgattgtgg
      301 tgctgctggc gggcggacag ggagaaaggt tgtggccgct gacgcgcgat cgcgccaagc
      361 cggcggtgcc gttcggcgcc gtttatcgca tcattgatat cacgctttca aattgcgtga
      421 actccggcct ccgccgcgta tttgtgctca cgcagtataa agctctgagt ctgagccggc
      481 acgtccgggc cggctggtcc gggctggccg ggcttggcga gtacatcgaa atattgccgc
      541 cgcagatgcg ggtttcgaaa gagtggtatc agggcaccgc cgacgccgtg taccagaaca
      601 tttattcgat cggcagcgaa cgttcgaaac acgtcttcat cctctccggc gaccacatct
      661 acaaaatgaa ctacgccaag atgctgcagc agcatctcga ttccggcgcg gaagtaaccg
      721 tcggaacgat tcagattccc accaaggagg cagcgcggca gtttggcgta attgaagtag
      781 ataaggattg gcgcatcgtc ggttttgagg aaaagccggc cgcaccgaaa gaatcgcccc
      841 acaatcccgg acgctgcaac gcatccatgg gcatctacat tttcaatacc cagctgctga
      901 ttcccgtatt actggcagat tcggaagatc cgaagtccca tcacgatttt ggcaaggaca
      961 ttttgccgcg catcatttcg aagtaccgcg tcttcgctta caattttgtg gacgagaaca
     1021 gcagcgagtc gctgtactgg cgcgacgtgg gaactatcga cgcatactac gaagccaaca
     1081 tggacctggt aagcgtctcg ccgattttca atttgtacga tcattattgg ccgctgcgga
     1141 cctggcagca tcagtacccg ccggcaaagt ttgtgtttgc cgatccggac cgcatgggcg
     1201 cggcggtcga ttcaatcgtg gccggaggtt cagtcatatc tggcggaaaa gttaccagaa
     1261 gcgtggtggg taacaacgtc cgcgtgaata gctattgcga cgtgagcgac agcattctgt
     1321 ataaccacgt aaacgtcgga aggcattcga agatccggag agctatcatt gaccggcacg
     1381 tcagtctgcc ggagcacacg gagatcggct tcgacgtgga agcggataag cggcgctatc
     1441 acgtaacgga gtcgggcata gtagttgttg tgcgtcagga gtcgctgatc gaggagccag
     1501 agaccgctta gtcggaaggg accatatgtt tattgccaca aaggatttga ttttgccgag
     1561 caccaccacc gggtcgtttc cgcggccacg atggtttgac gtgtgcatgt ggggcaagcc
     1621 gctcgatacg tgcatgctgg acgtgcgttt ccgcgaaaaa tttcaagatg cgctggcggt
     1681 tgtgatcagc gatcaggagc gctccggcct ggacatcttg agccatggcg attttcatgt
     1741 ggacgaggat ttggccggtc gagcgtggca tcactatccg ctgcaacgct gggcaggatt
     1801 cgagggcgat tatctgcagc ctgaggaaac cacggcgccc tggctgcact atccgcctgg
     1861 tacattgctg aatgaaattt acaccggctg gcgctggccg cacgtagtcg acaagatcga
     1921 acaccgcccg ctgggttatg caaagatctg gcgattggcc cagtcgaaaa cacgcaaacc
     1981 agtcaagttc ggcacctgct gctcgcaggt catggcgctg ttcctggaca ttcacacacc
     2041 gaaatacaag gacaagcggc aaattatctg ggacatggcc gaggccatga ataaggagct
     2101 gctggcgttg cgcgacgccg gctgccgctg cattcagatt gaagagccga cgttccactt
     2161 catggccaac acgttcggca aggatcatga cgaagtgaaa ttcatgattg atgcgttcaa
     2221 ccgcgaagtg caggggctcg acgatgtgga gctgtggatt cacacctgct ggggaaatcc
     2281 caacatgcag cgcgtgatgg aagacactag ctacgcgaat tcgattgaga tctatctgga
     2341 acgctgccgc ggcgacgtat ggacgctgga aatgaaagat cgcaatcaaa aggatctgga
     2401 gttgttcgcg ccgttcaaga gcaatttgaa gaagaaaatt gccatcggcg tggtgagtca
     2461 ccgccagttg caggcggatc gtccggagga agtcgcggcg gaaattcgca aagcgttgaa
     2521 gtacatccca gctgaaaagc tcatagtgtc aaatgattgc ggatttgggc gccagggctg
     2581 caaccgtgag atcgcttttt ataaagcgag tgccatcgcg caggcagcca acatcgttcg
     2641 caaggagctg ggattgccaa cgacgtatat accggcggcc gaccctgggt tgcaggtgga
     2701 tgtggtccca ggccgcgcgg cgaaagcggg ccaagcttcc tgatcgaaac aaaagttatt
     2761 tcgtggcttc ggcccactcc tgctcggcgc gatggagcag cgcatcgcgg tcttccggca
     2821 ggatccagcg ttgtttaatt aattcatcta cagcgtgggc gtaccgggtg acgtagtctt
     2881 cgcgcccgat gtagcgctca gcaattgact tgcgcggatc gcctgaattt ttgcgctcag
     2941 cggcagtttt aggaaatggc aaatacgaaa tttcgaaggc cacacgttca ctgggcgcgc
     3001 cgatggaaga gtcccgaagg ttccagcttg cataggtcgc taacggaacg gaaagctccg
     3061 gtaaacgcac cccatcgcgt tcgttgccgt cggcatccac ctgcggcacc agcaccggat
     3121 aaagc
//
LOCUS       DASTPM010000070         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1155710, whole genome shotgun sequence.
ACCESSION   DASTPM010000070 DASTPM010000000
VERSION     DASTPM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155710"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFR42_01255"
     CDS             <1..649
                     /locus_tag="VFR42_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367804.1"
                     /translation="AKAVAMAPNERDRLHTEFQKALVYFWEGRASQGLRAVNDVEQKA
                     RAAKEPYSLFEAQEALALLAATSRERLQKLSQIEATFSVPVENMGDADRNPTLAAIWR
                     DQARENAQQNKLDDARRAVQKLEQLAAKSRDLIVEDYYESARGYLYFAQKNFQDAADE
                     LSADPHSPVAVKWLAVAREKAGDLKGADVAKLRLKYLRAPTAEWYLATHSGSAAN"
     gene            complement(754..1002)
                     /locus_tag="VFR42_01260"
     CDS             complement(754..1002)
                     /locus_tag="VFR42_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0367805.1"
                     /translation="MSGLVYWIVVGLIAGFLAGKVMKGGGYGILMDIILGMLGGIVGG
                     WLFGLLGISAGGGIIGGILVAFVGAVVLVWLTRMLKRA"
     gene            complement(1058..2173)
                     /locus_tag="VFR42_01265"
     CDS             complement(1058..2173)
                     /locus_tag="VFR42_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU0367806.1"
                     /translation="MRRALILVMLSLSWFAALMKADQVTLRNGDRLTGTIVKTDDETK
                     TLLIKTELAGDVTVQWDAVTGIVSSQPLHLTLSDGRVVVGKVTTADGKLEVATKDAGE
                     VPAEHASIKAIRSDKEQAEYDRLQHPRLRDYWGGLFDLGLSVTQGNSATSALSIAGKA
                     ARVVPKNKLSLYYTQIYAKDSNQDPSATTANAIHAGIRYEFNLTPKIYLFAFTDFDSD
                     ALQNLDLRNVLGGGLGYHIINAKKTQFDVFGGGSFNQEYFSSYLTANPAPPPVEILVP
                     SESRHSAEVLVGESLSTKIGSRSTFTEQLTFFPNLSSPGDYRVNFDATATTKLKTWLS
                     WQVTFSDRYISNPPLNLKGNDLLLSTGLRLTFGRGIF"
     gene            2368..>3272
                     /locus_tag="VFR42_01270"
     CDS             2368..>3272
                     /locus_tag="VFR42_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU0367807.1"
                     /translation="MSLWLHRRAFLSLMAAVVFTGCASPPAPKELVQATPRKPTIVFM
                     TDFGTANDSVAICKAVIAGIAPEARIADITHQVTPFQIEEASRFLYGATPYYPSGTIF
                     LVVVDPGVGTSRKAVIVKTKKGQFFVLPDNGLASAVVDRDGLVGAREITNQNWMIQAA
                     VSSTFHGRDIFSPAAAHLAEGWDYTIAGPEVPQLVRLTPKTASVDEKGVTGEIIGLDD
                     PFCSLISDIPKEDFEKLGYKMGDRVSLLLNKKPITLPFAKTFMDVPVGEPLLYVDSRG
                     RMTIAVNQGNYSKKFGVEPGASIVVP"
BASE COUNT          694 a          988 c          869 g          721 t
ORIGIN      
        1 cgccaaggcc gtcgcaatgg ctccgaacga gcgcgatcgc ctgcacactg aatttcaaaa
       61 ggcgctggtc tatttttggg aaggccgcgc ctcgcaaggc ttgcgtgctg tgaatgacgt
      121 ggagcagaaa gcgcgcgctg cgaaagagcc ttactcgctc ttcgaagcgc aggaagcgct
      181 ggcactgctg gccgctactt cgcgcgagcg cctgcaaaaa ctcagccaga tcgaagccac
      241 tttcagcgtt cccgtggaga acatgggcga cgccgaccgc aatccgactc tcgcggccat
      301 ctggcgcgac caggctcgtg agaacgccca gcaaaataag ctcgacgacg cccggcgcgc
      361 ggtgcaaaag ctagagcaac ttgccgccaa atcccgtgac ctgattgtgg aagactatta
      421 cgaatccgct cgcggctacc tgtattttgc acagaagaat ttccaggatg ccgctgacga
      481 actctctgcg gacccgcatt cgccggtcgc cgtcaaatgg ctggctgtcg cccgcgaaaa
      541 agccggcgac ctcaaaggcg cggacgtcgc caagctccgg ctaaaatatt tgcgtgctcc
      601 gactgcggaa tggtacctgg ccacgcattc cggcagcgcg gccaactgag tttgccccaa
      661 acgcgaaccg tgccggtctg tctaccccct ggcaggctgg tgcggctcgc cccctggacg
      721 tcccgctaac tcgaatcgct tggaaaaatt ggttcaggct cttttcagca tgcgcgtcag
      781 ccaaaccaga actaccgctc cgacaaacgc tacgagaatg ccgccgatga ttccgcctcc
      841 cgcggaaatt cccagcagtc cgaacagcca accgccgaca attcctccca gcattccgag
      901 tatgatgtcc atcaagattc cgtagccacc gcccttcatg acttttccgg ccaggaatcc
      961 ggcaatcaac ccgactacaa tccaatagac cagtccgctc atggcagcct ccctgagtgt
     1021 cgtggagaaa tgaacgcggc gtgcgatgtg caaggtttta gaagattcct cgcccaaacg
     1081 tcaagcgcaa tccggtcgac agcaacaaat cgttgccctt cagatttaac gggggattgc
     1141 tgatgtagcg atcgctgaat gtgacctgcc aactaagcca cgtcttcagc ttagtggtgg
     1201 cggtcgcatc gaaattcacg cggtaatctc cgggcgaact caaattgggg aagaacgtca
     1261 gttgctcggt gaacgtggag cgcgacccga ttttggtgct cagtgattcg cccaccagca
     1321 cctcggcgct gtgccgcgat tcggaaggga caagaatttc gaccggcggc ggcgcgggat
     1381 ttgccgtcag gtatgacgaa aaatattcct gattgaagct gccgccgcca aacacatcga
     1441 actgcgtctt cttggcgttg atgatgtgat agcccaagcc tccgcccagc acgttgcgca
     1501 aatcaaggtt ctgcagggcg tccgaatcga agtcggtaaa cgcgaacagg taaatcttcg
     1561 gagttaggtt gaattcgtag cggattccag cgtggatggc gttcgccgtg gtcgccgagg
     1621 gatcttggtt gctgtctttc gcgtaaatct gcgtgtagta cagcgacagc ttatttttgg
     1681 gaaccacccg cgccgccttt cccgcgatgc tcagcgccga tgtcgcagag ttgccctgcg
     1741 tgacgctcag acctaaatcg aacagtccgc cccaataatc ccgcaaacgt ggatgctgca
     1801 accggtcata ctccgcctgt tctttgtcgc tgcggatggc tttgatggac gcatgttccg
     1861 cgggtacttc ccctgcgtcc ttcgtcgcca cttccagctt tccgtccgcc gtggtgacct
     1921 tgcctacgac cacgcggccg tcggaaaggg tgaggtgcag cggctgtgac gaaacaattc
     1981 ccgtgaccgc atcccattgc acggtcacat cccccgccaa ttccgtcttg atcagcagag
     2041 tcttggtttc atcatccgtt ttaacgatcg ttccagtgag ccggtcgcca tttcttaacg
     2101 tcacttgatc ggctttcatc aatgcagcga accatgacaa cgacagcatc accaggatta
     2161 acgctcgtcg catgcggatt tctccttgcg caaacttgag gatgtacttc gaggccgcgg
     2221 gcagggcctt acaaaaagcg attggctgaa gcgttcaaag tttacttgaa gcgcctagga
     2281 cttcccactt aatcgtgagg cggcgcgcat gctcctttcg caactgcttc cgcttatact
     2341 gtttcgacaa acggaaaagg agtctcgatg tcactctggt tgcatcggcg cgcgtttttg
     2401 tctctgatgg ctgccgtagt cttcacagga tgtgcgtctc cgcctgcccc caaggagttg
     2461 gtacaagcaa ctccgcgtaa gcccactatc gtttttatga ccgacttcgg cactgccaac
     2521 gactcggtgg ccatctgcaa ggcggttatc gcgggcatcg ccccggaagc gcggatcgcc
     2581 gacatcaccc atcaggtcac cccctttcaa atcgaagaag cctcgcgttt cctctacggc
     2641 gctacgcctt actatccttc gggcaccatt ttcctcgtgg tggttgatcc cggcgtaggc
     2701 acctcacgca aggcagtcat cgtcaaaacc aagaaaggtc agttctttgt cttgccggac
     2761 aacggcctcg ccagcgccgt agtagaccgc gacggtttag tcggcgcgcg cgaaatcacc
     2821 aatcagaatt ggatgattca ggccgctgtc tcttctacgt ttcacggtcg cgatattttt
     2881 tctcctgccg ccgcgcatct cgccgaaggc tgggattaca ccatcgccgg cccggaagtt
     2941 ccccaactcg ttcgccttac tcccaaaact gcctccgttg acgagaaggg tgtgaccggc
     3001 gaaatcatcg gcctcgatga tcctttttgc tctctcattt cggacatccc caaggaagat
     3061 ttcgagaaat tgggatataa aatgggagac agagtttccc tgcttttgaa caagaagccc
     3121 atcacgctgc ccttcgcaaa gacgttcatg gatgtgcctg tcggcgaacc gcttctctat
     3181 gtcgattcgc gcggacggat gacaattgca gttaatcagg ggaactattc gaagaaattt
     3241 ggcgtcgagc ctggggcttc catcgttgtc cc
//
LOCUS       DASTPM010000071         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1271092, whole genome shotgun sequence.
ACCESSION   DASTPM010000071 DASTPM010000000
VERSION     DASTPM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271092"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFR42_01275"
     CDS             <1..621
                     /locus_tag="VFR42_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367808.1"
                     /translation="KGQPITALSGSDANANGDVAGDRTIFNPNGSGLTGSAVNAVCNA
                     GPGGATSIVGIDPISPFGAWQCGTEDDSNIVGYVVAPATVGVDAGTIDPTARFIQAQV
                     GSKTNVGRNTVQTPGLNVWNIGLLKTTSLGERLKVQFRAETYNTFNHRNFSIGLPTNN
                     GNIDSTTNPNPLSTAYPFVTAGPLFLNNHQFTGGSRTMQLGLKLIF"
     gene            728..1666
                     /locus_tag="VFR42_01280"
     CDS             728..1666
                     /locus_tag="VFR42_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0367809.1"
                     /translation="MNPMKLSTALISRVWLVSMCLLIMDSSNEPAQGSDAAAEASAAY
                     EAHEWAKATRLYEGLLKEHPEVPRLWFRLADSQQQLGQFDQALTTAQNGLKAGAPPIF
                     VEFLIGEIYAQKNDKEKAFEHLKIAVDNGYNQPEQFDKVKHLDSLRSDTRFAQLKQQA
                     QHNLKPCADTSENRQFDFWIGEWNVETTQGGVPAGESKIELILGDCVIQENWQSNGNP
                     YAGKSYNMYNAALKRWEQYWVDNSAGNIFFYGGLKDGMMDYWTDQIPQPSGPPLKRHL
                     QFFKLGPDKVRQFSQGSTDGGKTWKSEYDFTYRRKK"
     gene            complement(1677..>3251)
                     /locus_tag="VFR42_01285"
     CDS             complement(1677..>3251)
                     /locus_tag="VFR42_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0367810.1"
                     /translation="YIFVFQDIRGRYGSEGKFEMLHTAPDAKGVNESTDAYDTIEWLV
                     KNVPKNNGRVGMNGISYSGYLVTMGMVNPHPALKAVSEQACMGDTWLGDDYFHNGAFR
                     LSYGFEYTALLESSNENFSFPFDRFDMFEWYLRLGPLSNANEKYFHGKIPTWNNFVAH
                     SSYDDFWKRHAVAYTLHEPTVPNLNVAGWWDQEDFFGPIMTYGRLEKSDRKNNNFLVV
                     GPWNHGGWAGGKGSSLGDIPFHNDTGVYFRQKIEAPWFAYWLHDKGTLPVKEATTFQT
                     GSDSWTSYDSWPPKEAQTKHLYFREDGKLSFDAPVTDSKDAADSYISDPAHPVPYRRR
                     PVDMTYPQDHPGSWFTWLVQDQRFVDGRPDVLTWQTDTLHDDVTVTGSVVAKLFASTT
                     GSDADWVVKLIDVYPEKVEEDWKLSGFELMVADEIFRGRYRKSFEMPEAIAPNQITPF
                     TVDLHSTNHVFKKGHRIMVQVQSTWFPLYDRNPQKFVPNIFEAKESDYQKATQTIYRS
                     KEHPSSVEVMVLPNGK"
BASE COUNT          717 a          857 c          970 g          707 t
ORIGIN      
        1 aaaggtcagc cgatcacggc gctctctgga agcgatgcga acgcgaatgg agacgtggct
       61 ggcgatcgca ccatcttcaa tcccaacggt agtggactca cgggctcggc ggtcaatgcg
      121 gtgtgcaatg ccggacctgg cggagcaacc agtatcgttg gcatcgatcc gatcagtccc
      181 tttggcgcgt ggcagtgcgg caccgaggac gactccaaca tcgtcgggta tgttgtcgcg
      241 ccggctacgg taggcgtgga tgcgggaacg attgatccca ctgcgcgctt catccaggct
      301 caggtcggca gcaagaccaa cgtgggccgc aacaccgtgc aaacgcccgg tctgaacgtg
      361 tggaatatcg gtctgctgaa gaccaccagc ctgggcgaac ggctcaaggt gcaattccgc
      421 gcggaaacgt acaatacgtt taaccatcgc aatttcagca tcggattgcc gacgaacaac
      481 ggcaatatcg attcgacgac aaaccccaat cctttgagca cggcgtatcc gttcgtgacg
      541 gccgggccgt tgttcctcaa caaccatcag ttcacgggcg gcagccgcac catgcaactg
      601 ggcctgaaac tgatcttcta acccgggtgg catacgtgct agcgagggag tcaatttggc
      661 tccctcgctt tctttttgtt cagcgagcgt ctaatggcgg caggcgaggt ggcgatggga
      721 gctttccatg aatccaatga aattgagcac agctctgatt tcgagagtgt ggctcgtaag
      781 catgtgtctg ctcataatgg atagttcgaa cgagccggcg caagggtccg atgcggctgc
      841 ggaggccagc gcggcgtatg aggcacatga gtgggcaaaa gccacgcggc tttacgaagg
      901 gctgctgaag gagcatcctg aagtgccgcg gctgtggttc cggcttgcgg attcgcagca
      961 gcaactcggg cagttcgacc aggctctgac gactgcgcag aacggattga aagcgggagc
     1021 gccgccgatt tttgtcgagt ttctgatcgg cgaaatttac gcgcagaaga acgataaaga
     1081 aaaagctttt gagcatctga agattgcagt ggataacggc tacaaccagc cggaacagtt
     1141 tgataaggtc aagcatctgg attcgctgcg aagcgacacc cgatttgctc agttgaaaca
     1201 gcaagcgcaa cacaacttga aaccgtgcgc ggacacctcg gaaaaccggc agttcgattt
     1261 ctggattggc gagtggaatg tagaaaccac gcagggtggc gtgccggccg gcgaaagcaa
     1321 aatcgaattg attctgggcg actgcgtcat tcaggaaaac tggcagagca acggcaatcc
     1381 ttatgccggc aagagctaca acatgtataa cgccgcgcta aagcgttggg agcaatattg
     1441 ggtggacaac agcgcgggca acatcttctt ctacggtgga ttgaaagacg gcatgatgga
     1501 ctactggacg gaccagatcc cgcagcccag tgggccgccg ctcaagcggc atctgcaatt
     1561 tttcaagctc ggtccggaca aagtgcggca gttcagtcag ggatcgaccg acggcgggaa
     1621 aacctggaag tcggaatacg acttcaccta caggcgcaag aaatagaggc ggcagcttac
     1681 ttgccgtttg gcagcaccat cacttccacg cttgagggat gctccttcga gcggtaaatg
     1741 gtttgcgtag ctttctggta gtccgattct ttggcctcga agatattcgg gacaaatttt
     1801 tgcggattgc ggtcatagag cgggaaccag gtgctctgca cctgcaccat gatgcggtgg
     1861 ccctttttga aaacgtgatt cgtcgagtgc aagtcgacgg tgaagggggt gatctggttc
     1921 ggcgcaatgg cttccggcat ttcgaaggac ttgcgatagc ggccgcgaaa aatttcgtcg
     1981 gccaccatca gttcgaaacc ggaaagcttc cagtcttctt ccactttttc gggatacacg
     2041 tcgatcagct tcaccaccca atcggcgtcg ctgccggtgg ttgatgcaaa aagttttgcg
     2101 accacgctgc cggttacagt gacgtcgtcg tgcaacgtgt cggtctgcca ggtcaaaacg
     2161 tcggggcgtc cgtccacgaa gcgctgatct tggaccagcc aggtgaacca gctaccggga
     2221 tgatcttgcg ggtaggtcat gtcgaccggc cgacggcggt aaggcaccgg atgtgcggga
     2281 tcgctaatgt agctatcggc cgcgtctttg gaatctgtaa cgggcgcatc gaacgagagc
     2341 ttgccgtctt cccgaaaata caggtgtttg gtttgcgctt ctttgggcgg ccaggaatcg
     2401 tagctggtcc agctatcgct gcccgtctgg aatgtggtcg cttcctttac tggcagcgtg
     2461 cctttgtcgt gcagccagta ggcgaaccac ggcgcttcaa ttttttgacg gaaataaacg
     2521 ccggtgtcgt tgtggaaggg aatgtcgccg agcgaggaac ccttgccgcc ggcccatccg
     2581 ccatggttcc agggccctac taccaggaaa ttattatttt tccgatcgga cttttccagc
     2641 cgtccgtagg tcatgatggg cccaaagaaa tcttcctgat cccaccagcc ggccacgttc
     2701 aagttgggga cggttggttc gtgcagcgtg taagccaccg catggcgctt ccagaagtcg
     2761 tcatagctgc tgtgcgcgac aaagttgttc cacgtcggga tttttccgtg gaaatatttc
     2821 tcgttcgcgt tagagagggg gcccaggcgc aggtaccact cgaacatgtc gaagcggtcg
     2881 aatggaaaac tgaaattttc gtttgagctt tcgagcagcg ccgtgtactc gaagccatag
     2941 ctcaggcgaa acgcgccgtt gtggaagtag tcgtcgccta gccaggtatc ccccatgcag
     3001 gcctgctcgg aaactgcttt cagcgccgga tgtgggttga ccatgcccat cgtcacgaga
     3061 tagcccgagt acgaaatgcc gttcatgccc acacggccgt tatttttggg cacatttttg
     3121 accagccatt cgatggtgtc gtaggcgtcg gtgctttcgt tcacgccttt cgcgtccggc
     3181 gcggtgtgca acatttcaaa tttgccttcg gagccgtagc ggccgcgaat gtcctgaaag
     3241 acaaaaatgt a
//
LOCUS       DASTPM010000072         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1300075, whole genome shotgun sequence.
ACCESSION   DASTPM010000072 DASTPM010000000
VERSION     DASTPM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300075"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFR42_01290"
     CDS             <1..252
                     /locus_tag="VFR42_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEU0367811.1"
                     /translation="RYVLPPAIFGCLERTQPGAGGEIQLTDAMKLLAKEQGLWAYIYE
                     GISYDAGEKLGFLKATVELALQNEELGEAFRGYLKSLKL"
     gene            complement(266..583)
                     /locus_tag="VFR42_01295"
     CDS             complement(266..583)
                     /locus_tag="VFR42_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU0367812.1"
                     /translation="MPLYEYQCAKCGKRTEKIESVSGPHLKRCPLCGGKVERMVTAPA
                     IQFKGSGWYVTDYAGKSSPKEGATAEVGSTDKKDSSERKDSASKESSSAKDSKDKKPA
                     KKK"
     gene            664..1539
                     /gene="rsmI"
                     /locus_tag="VFR42_01300"
     CDS             664..1539
                     /gene="rsmI"
                     /locus_tag="VFR42_01300"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU0367813.1"
                     /translation="MEPQSNGRTGCLYLVATPIGNLEDITLRALRTLKEVDQIACEDT
                     RHTQKLLSHYHIQKALVSYHEHNEMTRAPELVLAMEQGARIALVSDAGMPLVSDPGYR
                     LVTLSLRHHLPVVPIPGAAALLAALSASGLPNEEFLFAGFLPARTGERRRALERLRIE
                     DRTIIFYEAPHRIEETLADAREVLGDRPACLAREVTKLHEEFRRGTLSELAASLSDKP
                     ARGEITLLIGAVPPEERAAQRDTSQSLAERVDELIRQAKLDRKEALKLAAKERGMTKR
                     AAYQELHLHLREPEA"
     gene            complement(1549..1848)
                     /locus_tag="VFR42_01305"
     CDS             complement(1549..1848)
                     /locus_tag="VFR42_01305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0367814.1"
                     /translation="MQVTGRDSRGVVFTEETSSENLCRGGAAFVTRFDLPIGADIDIR
                     IPTSSQQAWKREDTNFATRGRVVHVSEATGPGERLVGVQFTGPRFQRVFRSESAA"
     gene            complement(1874..2935)
                     /gene="dusB"
                     /locus_tag="VFR42_01310"
     CDS             complement(1874..2935)
                     /gene="dusB"
                     /locus_tag="VFR42_01310"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU0367815.1"
                     /translation="MIPAEYKVRNLSIRPATVLAPMAGVTDTLFRRVIRGLGGCGLLM
                     TEFTSSEGITRSAKKTLRYLYFQPDEHPITAQLFGANPQVMADAAKMVEDHGFDAVDI
                     NLGCPAKKVVKCGGSGLLRDLALLGEIFKAVRAAVRIPLTIKLRAGWDENSIVAVEVA
                     RLAESIGVEAVAVHPRTRVQGYSGQADWNIIAAVKQAVKIPVIGNGDIQTPEDAARIV
                     AETDCDAVMIGRAAATNPWIFSQMAEYRETGRYQQPTDADRHRLLSGYYKEIQSANQS
                     DAIGKMKQFACWFTHGVGNGSELRRTVHAARTPSEVLDRVERFFESPAAARTAAGVSV
                     ANGEPAEDASEHAPLETAN"
     gene            complement(2956..>3207)
                     /locus_tag="VFR42_01315"
     CDS             complement(2956..>3207)
                     /locus_tag="VFR42_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367816.1"
                     /translation="DLDSKMAAARTALAQESGDEEWSAYSGALQRKFLKLDRYLESGE
                     KKKTHQASPSTEALAAQAAVQPASQPVTPPAGEKEGQRT"
BASE COUNT          611 a          983 c          903 g          710 t
ORIGIN      
        1 cgctacgtcc tgcctcccgc gattttcggc tgcctcgaac gcactcagcc gggggcgggc
       61 ggcgaaattc agctgactga tgcgatgaag ctcctcgcca aggaacaagg tttgtgggcg
      121 tacatctatg aagggatttc atacgacgcg ggtgagaagc tgggtttcct gaaagcgacc
      181 gtggaattgg cgctacaaaa cgaggaactt ggagaagcgt ttcgcggcta cctcaaatca
      241 ttgaagttgt agccgaggta ctttcttatt tctttttcgc gggcttcttg tcttttgaat
      301 ccttcgccga cgaactctcc ttcgacgctg aatcttttct ctcggaagaa tcctttttgt
      361 ctgtcgaacc tacttccgcg gtcgcccctt ccttggggct cgatttaccg gcatagtctg
      421 tcacgtacca acccgatccc ttgaactgaa tcgccggcgc ggtgaccatg cgctccacct
      481 tgccgccgca gagcgggcac cttttcagat gcgggccgga aacgctttca atcttttcag
      541 ttcgcttacc gcacttcgcg cattggtact cgtacaatgg cactgcccat cctccaaatg
      601 tcggtgaaag acgaagtata taccacgcgg ctgcagggcg aaaacgcgtg ttagactgct
      661 gccatggagc cgcagtccaa tgggcgcacg ggttgcttgt atctggttgc gacaccgatc
      721 ggcaatctgg aagacatcac gctgcgcgcg ctgcgcacct tgaaagaagt ggaccagatt
      781 gcctgcgagg atacgcgcca cacgcaaaaa ctcctgagtc attaccacat ccagaaagcg
      841 ttggtcagct accacgaaca caacgaaatg acccgcgcac ccgagctggt gctggcgatg
      901 gagcaaggcg cccggatcgc gctggtcagc gacgccggca tgcccctggt cagcgatccc
      961 ggctaccgcc tggtgactct ttctctgcgg catcatttgc cggtcgtgcc gattccgggc
     1021 gcggcagctc tgttggctgc gctttcggcg tccggtttgc cgaatgaaga gttcctgttc
     1081 gcgggattct tgccggcgcg cactggtgaa cgccgtcgcg cgctagaacg cctgcgcatc
     1141 gaagatcgca caatcatttt ttacgaggcg ccgcatcgga ttgaagaaac tcttgccgat
     1201 gctcgggaag ttttgggcga tcgtcccgct tgcctggccc gcgaagtgac caagctacac
     1261 gaagagttcc gccgtggcac gctgtcggaa ctggccgctt cgctttccga caagccggcg
     1321 cgtggagaaa tcaccctgct catcggagcc gtcccgcctg aggaacgcgc agcgcagcgt
     1381 gacacttcgc aaagtctcgc cgaacgcgtt gacgaactga tccgccaggc caaactcgat
     1441 cgcaaggaag ccctgaaact cgcggccaaa gagcgcggaa tgaccaagcg cgcggcttat
     1501 caggaattgc atttgcattt gcgcgaaccg gaagcctgat tactggctct acgccgccga
     1561 ctccgaccga aacacacgct gaaagcgtgg gccggtaaac tgcacgccca ccaatcgttc
     1621 gccgggacct gtcgcttctg aaacatgcac tacgcgtccg cgcgttgcaa aattcgtgtc
     1681 ttcgcgcttc caggcctgtt gtgacgacgt aggaatgcgg atgtcgatgt ccgcgccaat
     1741 gggcaggtca aaacgcgtga cgaacgcagc gccgccgcgg caaagatttt cgctcgaagt
     1801 ttcttcggta aagaccacgc cgcgggaatc gcggccggtc acttgcatgg gcaagcggac
     1861 ggcaatccgt cgttcagttc gctgtttcga gaggtgcgtg ctcgcttgca tcttcggccg
     1921 gctccccgtt tgccacactc acacctgcag cagttcgcgc ggcggccggg ctctcgaaaa
     1981 aacgttccac tcgatcgagc acttcgctgg gagtccgggc tgcgtgaacc gttcgtctca
     2041 gttcgctgcc gttgcccacg ccgtgcgtga accagcatgc gaactgcttc attttgccta
     2101 tggcgtcaga ctgatttgca gactgaatct ctttgtagta gccagaaagt aaacggtgcc
     2161 ggtcggcgtc cgtgggctgc tgatagcggc cggtttcccg gtattccgcc atttgcgaaa
     2221 aaatccacgg atttgttgcg gcggcgcgtc cgatcatgac cgcatcgcaa tcggtttccg
     2281 cgacaatgcg cgcagcgtct tcgggagtct gaatgtcgcc attgccgatc accggaattt
     2341 tcaccgcttg tttcaccgcc gcgatgatgt tccagtccgc ctggccgctg taaccttgca
     2401 cgcgcgtgcg cgggtgcacc gccaccgctt ccactccgat ggactcagcc agcctggcta
     2461 cctctacggc aacgatcgaa ttttcgtccc agcccgcgcg cagcttgatg gtcaggggaa
     2521 tgcgcacagc ggctcgcacc gctttaaaaa tttcgcccag tagcgctaaa tcccgcagca
     2581 gcccggaacc gccgcacttc acaacctttt ttgccgggca gccaaggtta atgtccactg
     2641 cgtcgaaccc gtgatcctcc accatcttcg ccgcgtcagc catcacttgc ggattcgcac
     2701 caaacagttg cgcggtgata gggtgttcgt ccggctggaa ataaaggtaa cgcagggttt
     2761 tcttggcgct gcgagtgatt ccttcggagc tggtgaactc cgtcataagt aatccgcaac
     2821 cgcccaagcc gcgaatcaca cgacggaaga gcgtgtccgt aacgccggcc attggcgcca
     2881 gcaccgtcgc cggccgtatg gacaggtttc tgaccttgta ttcagcggga atcattactt
     2941 ttactgtagc gcagcttacg tgcgctggcc ttctttttca ccggctggtg gtgttaccgg
     3001 ttggctagcg ggttgcactg ctgcctgagc ggcaagcgct tcagtcgacg gcgacgcttg
     3061 gtgggtcttt ttcttttcgc cgctctctag ataacggtcc agcttcagga attttcgctg
     3121 cagtgctccg ctgtacgccg accattcttc atcgcccgat tcctgagcca gcgcggtgcg
     3181 tgccgccgcc atcttggaat ccagatc
//
LOCUS       DASTPM010000073         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_867587, whole genome shotgun sequence.
ACCESSION   DASTPM010000073 DASTPM010000000
VERSION     DASTPM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867587"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..355
                     /locus_tag="VFR42_01320"
     CDS             38..355
                     /locus_tag="VFR42_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367817.1"
                     /translation="MGIETRDSAQYRFVRGQLQRAKKPQQIRGLNQNHNHEMKEIFKG
                     AATRASCGVGPLRDFYVALLDKGMKPEMARLTLARKIAAIALILWKKEERFDAEQLKT
                     QAA"
     gene            950..1687
                     /locus_tag="VFR42_01325"
     CDS             950..1687
                     /locus_tag="VFR42_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367818.1"
                     /translation="MRTSSIVSTALLLVLAGLVQAQDGFILECPAGHTDVMKYFVMDQ
                     ARRTNKFMKGRPNSLYTEVFPDRDFAARGHWLWLKSATGHGFDVKVFDEGHVYMRATE
                     LTWTDNTSFKRFVKDLPISERCVPEGKAGPEIKVEDTRYDYYSSCKPYKLSKLGTAVN
                     DLDAPALMDVGSNVGQAWTRVLHYHYNCDEQFRNCSDEEQFYLANGFGLWQWKHYRHG
                     ALMKSTLMNEIEAGNATGKLPCEDSYR"
     gene            1844..2908
                     /locus_tag="VFR42_01330"
     CDS             1844..2908
                     /locus_tag="VFR42_01330"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0367819.1"
                     /translation="MIIIGVDFHPEFQEMASVDTDTGEYQEKRLTHPEEAEQFYRSLA
                     SVGQTVRVGMEASGHGRWFERLLEELRIELWRGDATVIRAKRGRKQKTDREDARHILK
                     LLLKDDFPRIWVPSWENRDLRQLLWHRHRMVQARTRVMNQLQAVALNEGLRCKKRLWR
                     EGGRQKLESFRLAPWASRRRRELLELLDRLNPMIAELSQAVEQEAEKCPEAKQLMTHP
                     GVGELTALAFVLIIGNTERFGCGKQVASYLGLVPLEESSGNRRRLGHITKQGSSIVRF
                     LLVEAAQVTVRKLPDWRSKYVHLMMRRGRKTAKVAMARKLAVRLYWMWRKGWDYEQWK
                     KFGSHVGQPGNRDGVRQNTE"
     gene            3362..>3628
                     /locus_tag="VFR42_01335"
     CDS             3362..>3628
                     /locus_tag="VFR42_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367820.1"
                     /translation="MKRLLLIVVLLLVVFAPCAYADSIPTFEITQLSLNIFPSLFPGN
                     VGISFTGPGLFGSGVGTGNCLSPPFWCAQGNIDGLLPGASLNPTL"
BASE COUNT          875 a          923 c         1068 g          762 t
ORIGIN      
        1 gttccgtagc aagcgacaac tgtggacgta cagtggcttg ggcattgaaa cgcgcgacag
       61 tgcgcaatac cgctttgtcc gcggacaact gcaacgggcg aaaaaaccgc aacagattcg
      121 tggtctcaat cagaatcaca atcatgagat gaaagagatc tttaaaggag ccgcgacccg
      181 agccagctgt ggggtggggc cgttgcgcga cttctatgtg gctttgctgg acaaaggcat
      241 gaagcccgag atggcacgcc taacgctagc acggaagatc gcggccattg ctttgatact
      301 ttggaaaaag gaggaacgtt tcgacgccga acaactgaag acgcaagccg cttgagcgtt
      361 gcacagaact ccagttgctc tccgaggtct tcatacctcg tgggtggcca atcggttctg
      421 cgacgctcgg gttcgaggga gagtatccat tcactcatta ggcgccgtgt gctgcattca
      481 gcaccgagtc tccgggtcaa tcctatgccc cctcgcacaa cccaaaaaag ccataggcct
      541 cgagcctcac acaggatgat ggttggcact gaaagccaac ccatgggctt gcgtagcttc
      601 gcaacggatc ggcgtcaagc gcccacggca agatgaaacc attccctgca aacccgcaac
      661 tggggttggc atcgccgctg caatacgcgg cgatccaaag cggcaaaaac caaaaacccc
      721 tccacgaagg ggacggggct ggatgtaagc caccagcagg tctccagagg ggagagggga
      781 agagtttttt cttgacatcc tcttttacag gatgagtgaa acagcgatgt ttcaacacta
      841 acggccgcca cccctggggc aggccctccc ccgagatgct caccggcgaa aaaagcttgc
      901 gattcacaca cgggaaccat caaacggatt gtcatctcag aggtgcggaa tgcggacatc
      961 ctcaatcgtc tcaactgcct tgttgctggt gctcgccggc ctcgtccagg cccaagacgg
     1021 attcatcctc gagtgtccgg ctggccatac tgacgtaatg aagtatttcg tcatggacca
     1081 ggcacgacgc accaataaat tcatgaaagg gaggccaaac tcgctctaca ccgaagtctt
     1141 tccggatcgg gatttcgcag cccgcggtca ttggttgtgg ctgaaaagtg caacgggaca
     1201 cggcttcgac gtaaaggtgt ttgatgaagg gcacgtttac atgcgcgcta ccgaactcac
     1261 ttggaccgac aacacaagct ttaagcgatt cgtgaaagac cttcccattt cggagcgctg
     1321 tgtgcccgaa gggaaggccg ggcccgagat caaggttgag gatacgcgat atgactacta
     1381 ttcctcgtgc aagccctaca aattgagcaa gcttggcact gcagtgaatg acctggatgc
     1441 tccagcactg atggacgtgg gcagcaatgt cgggcaggca tggactcgcg tgttgcacta
     1501 ccactacaac tgcgacgagc agttccggaa ctgttcggac gaagaacagt tttacctggc
     1561 taacgggttc ggcctatggc aatggaaaca ctatcggcat ggggccctca tgaaatcgac
     1621 gctcatgaac gagatcgagg ccggaaacgc cacggggaag ttgccttgcg aggacagcta
     1681 tcgatgagtg attgcatctt aactggcgca aacaggcgat ttcactcata accaaatggt
     1741 tatgagcgta attgattcca tgacaatgtc ggctcctagg tcccgagtaa agttagtggt
     1801 gccaaaagcc aaccaacaag gctcgggaag aaggagccga acaatgatca ttataggagt
     1861 agatttccac cccgagtttc aagaaatggc atcggtggat acggacaccg gagagtacca
     1921 agaaaagcgt ttaacacatc ccgaggaagc agagcagttt tatcgttcct tggcgtctgt
     1981 aggtcagacg gtacgtgtgg ggatggaggc cagcgggcac ggacgctggt ttgagcggtt
     2041 gctggaagag ttgcgaatag agctgtggag gggggatgcc acggtgatcc gagcgaaacg
     2101 aggacgcaag cagaagacgg atcgcgagga tgctcggcac attctcaaac tcctgctgaa
     2161 ggatgatttt ccgcggatct gggtgcccag ctgggagaat cgtgatctgc ggcaattgct
     2221 gtggcaccgc caccgcatgg tgcaggcgcg gacgcgggtc atgaatcaac tgcaggcggt
     2281 cgcgctgaac gaaggcctgc gctgtaagaa gcgtttgtgg cgggaaggtg gacgccagaa
     2341 actggagtcc ttccggttgg cgccgtgggc cagccggcga cgacgtgagc tgctggagtt
     2401 gttggatcga ttgaacccca tgatcgcaga gttaagccag gcggtcgaac aagaagccga
     2461 aaaatgtccg gaggcaaagc agctgatgac ccaccccggg gtcggtgagc tcaccgcact
     2521 ggccttcgta ctgatcatcg ggaacacaga acgctttggc tgcggcaagc aagtggcgag
     2581 ctatctcgga ctggtgccgc tggaagaatc gagcgggaat cgtcgacgac taggacacat
     2641 caccaaacag ggaagttcga tcgtgcgttt cttgctggtg gaagcagcgc aagtcacggt
     2701 gcgcaaactg ccagactggc gcagcaagta tgtccacctg atgatgcggc gaggacgaaa
     2761 gaccgccaaa gttgccatgg ctcgtaagct ggccgttcgc ttgtactgga tgtggcgcaa
     2821 gggatgggac tacgagcagt ggaagaagtt cggttcgcac gtgggacagc ccggaaatcg
     2881 cgatggtgtg cgacagaaca ccgagtaatt gattgggcgt cccactcccc ttttccgggg
     2941 gagtctgaag tagtaatcat gatcgcagtt gtgaccgaag agatgcatgg gtcggatcga
     3001 cgttctggcc tgaattgatt acgagcgagc cttggttggc aatggcaatt gtttttcaga
     3061 gagagcgaaa tggcgctctt cttcgagcgg atgcggcagt gtgctcaaaa agtgcttgac
     3121 accaccgaca ttgtcagaga agcgattttt cgccaactgt cagtccgcat tgagtgtaat
     3181 gggatatttc aacatctaca cctcggtgcg atctgtggac gtattttggt gaaacgtcta
     3241 ttttgaccac gtcagttggg tgtaaaattt gtgtccaatt cgatgttcca ctctgcggcc
     3301 acagcaagca atagcccagc gttgtggcac gaaggccgat tggtgaaagg agtacccaac
     3361 catgaaacgc cttctcttga tagtcgtgtt gctgcttgtc gtgtttgcgc catgcgccta
     3421 cgctgacagc attcccacgt tcgagattac gcagctcagt cttaacattt ttcccagttt
     3481 atttcccgga aacgtgggta tcagtttcac tggtccaggg ttattcggtt ccggcgtcgg
     3541 tactggtaat tgcctgtcgc ctcccttctg gtgtgctcag ggaaatattg atggactctt
     3601 gcccggcgct tcgctaaacc caaccctt
//
LOCUS       DASTPM010000074         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1098353, whole genome shotgun sequence.
ACCESSION   DASTPM010000074 DASTPM010000000
VERSION     DASTPM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098353"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..403)
                     /locus_tag="VFR42_01340"
     CDS             complement(71..403)
                     /locus_tag="VFR42_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367821.1"
                     /translation="MKSNSISRMAALFIALAMTVPVFAKPFTKTINLSQSAKFGKASL
                     QAGEYQLSIDGTKATVQKGKNTLGESEGRWEERDSKSEYTSILVGENGQIREVRFAGQ
                     KKVFVFSE"
     gene            911..1654
                     /locus_tag="VFR42_01345"
     CDS             911..1654
                     /locus_tag="VFR42_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0367822.1"
                     /translation="MRLLILSDLHANLTAFEAALAAAAGRWDGAVCLGDVVGYGPDPV
                     EVSQKLQTLTQEVIRGNHDKAVAGIMSSEDFNPVAKMAVDWTREVLSRELLRWLAELP
                     AGPRKSQGVVLIHGAFQDEDEYVFTPAQALEGLLDSSTTLSFFGHTHHQGGFSYHESD
                     IELLPLRPRASELFSALHLEPGYKYLLNPGSIGQPRDGDPRAAFAIADFEHNVVEFWR
                     VPYDIPAVQERMRDASLPDPLIHRLSLGR"
     gene            1667..2185
                     /locus_tag="VFR42_01350"
     CDS             1667..2185
                     /locus_tag="VFR42_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367823.1"
                     /translation="MQDAFESPLPAPQKEPEGIFRHPAVIALATLFGVAALYLLFFRG
                     PVPSSNSVKQRLPFGAAEQAYAPKLQLDNFAMSEAENFLHQEVKYLDGDIRNAGDRTL
                     TGIEVIVEFQDDMKQIALRESRQALGGPSAALAPGGTAHFQVSFDHVPPSWNMQMPTV
                     QVSGLQFATTSR"
     gene            2483..2827
                     /locus_tag="VFR42_01355"
     CDS             2483..2827
                     /locus_tag="VFR42_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0367824.1"
                     /translation="MNFSVKIRQQDHVALIDVCGHLTFFEVRALRDSVQSLLREKRNN
                     ILLNLSELKYLDSSGIGELARIYVAVVKQGGAMKVVGLAPKVEEILKITHLSQVFQEF
                     PDEQTALRSFAQ"
     gene            2960..3919
                     /locus_tag="VFR42_01360"
     CDS             2960..3919
                     /locus_tag="VFR42_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0367825.1"
                     /translation="MRESLLAFLRCPECGCTLSSRPSKTRGEEILAGDLACTQCAARF
                     AVQAGVPRMLRDDAPATRTQLSFGKQWQLHSQGSFERGPIYSKSKEECLDDFRKAFGL
                     RDLSGLRDCVILDAGCGSGELTADLGVAAPSATIIGMDFSVAAHVAFDRCRGIPNVHI
                     VQADLSRPPFSSETFDFVWSEGVIHHTPDTARSFSSLSHLVKSRGKLYVWIYSDRVTT
                     PYRLARKILRKPYLLPSAALYALSWSLALPLHLLNKMREMLRLSRVRHRLSSTAYSFY
                     DTLSPEFVNFHSQEEVHGWFARQHFDPVVFLTGTDIAACGTKK"
     gene            complement(4084..4614)
                     /locus_tag="VFR42_01365"
     CDS             complement(4084..4614)
                     /locus_tag="VFR42_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU0367826.1"
                     /translation="MPRRASLMQEPVLVLNATYEPINVTAVRRAMVLLLKGVAQAEEL
                     HVSEVHSAANSHKVPSVIRLLAYRHIPQQSRALSRKNILLRDRNTCQFCGRVFPSSEL
                     TLDHVVPRSRGGRSSWENLVACCYRCNNSKGDRTPEEAGFKLARRPRPFTLHTSRQLM
                     RMIGHRDERWRKYLFY"
BASE COUNT         1070 a         1436 c         1543 g         1127 t
ORIGIN      
        1 aagaaggaaa ccgcacggag cggcgatttc cttcttcgca ttgagaggag tcagttaaac
       61 accgtgagcg ctattcgctg aaaacgaaaa ccttcttttg accggcgaag cgaacttcgc
      121 ggatctggcc gttttcgccc acgaggatgg aagtatattc ggatttggag tcgcgttctt
      181 cccagcggcc ttcggactcg ccaagagtgt tcttgccctt ttgcacggtg gccttagtgc
      241 cgtcaatgga gagctgatat tcgccggcct gcagcgaagc tttgccgaat ttggcggatt
      301 gagaaagatt gatggtcttg gtaaacggct tggcaaaaac gggcaccgtc attgccaggg
      361 caatgaacag ggccgccatg cgagaaatgg aattgctctt catgcgtgtc accttatcgt
      421 gcagccgctt tccgcggctg gctccctttc gctcaagata gaaatgagcc gcaggggatg
      481 ggtctgaaga cagttgacac gccccatctg tgcgagtaga ttcgccagca cgacaccaaa
      541 gtttctggac taagcgccgg aaaaacgtgc gtgtcaaaat tgccccgcgg ctgccaccgc
      601 ggggtttttt ctatctcaaa cttgtacagc cccaaaatcg gccgcacccg tatagacgag
      661 tgagcgcgac cgaatgtttc gcggacggag aaaagttttt tgggaaagtt ggcgtgttga
      721 atacattgag acacaaggaa caaaaagtga cagatgtaac ggcagcgtaa cggctgaatt
      781 gttgctaaat ttagggaatg ttatgagcca aaatggtttc taacgcgtca cgtatccttc
      841 tttcgtatcg tgtttcgtgc atcgcggagc atgttccact atactcaggt gcgctctttg
      901 gacccgtgga atgcgactgc tgattctcag cgatctccat gccaatttga cggcttttga
      961 agcggcgctg gcggctgcgg caggccggtg ggacggtgcc gtgtgtctcg gcgatgtggt
     1021 gggttacgga ccggacccgg tagaagtttc ccagaaactg cagacgttga cacaagaggt
     1081 gatccggggc aaccacgaca aagcggttgc gggaattatg agttcggaag atttcaaccc
     1141 ggtggccaaa atggccgtgg actggacgcg cgaagtgctg tcccgtgaat tgctgcgatg
     1201 gctggcggag ctgccggccg ggcccaggaa atcgcaggga gtggtgctga ttcacggcgc
     1261 cttccaggat gaagatgaat atgtgttcac gccggcgcaa gcgctcgaag gcttgctgga
     1321 ttcgtcgacg acgctgagtt ttttcggcca cacgcaccac cagggcggat tttcgtatca
     1381 cgagagcgac attgaactgc tgccccttcg gccgcgcgca tcggaattgt tttctgcgct
     1441 gcacctggaa cccggttata agtatttgct gaatccggga tcgatcggac aaccgcggga
     1501 cggcgatccg cgcgcggcat ttgccatcgc cgatttcgag cacaacgtcg tggaattctg
     1561 gcgcgtgccg tatgatattc ctgcggtcca ggagcgcatg cgcgatgcca gcctgcctga
     1621 cccgctgatt caccggcttt cgctgggccg ctagaaacgg acaagaatgc aggacgcgtt
     1681 tgaatcgcca ttgccggcgc cgcaaaagga gccggaagga attttccgtc accccgcggt
     1741 catcgcgctg gcgacattat tcggcgtggc ggcgttgtac ctgttgttct ttcgcgggcc
     1801 ggtcccgtcg tcgaatagcg tgaaacagcg gcttccgttc ggcgcggctg aacaggcgta
     1861 cgcgccgaaa ttgcaactcg acaatttcgc catgagcgag gcggaaaatt tcctgcacca
     1921 ggaagtgaag tacctggacg gcgacattcg caacgccggc gaccgcacac tgaccgggat
     1981 tgaagtgatc gtcgagtttc aggacgacat gaaacagatt gcgctgcgcg agagccggca
     2041 ggcgctgggc gggccgtcgg cagcgctggc tccgggtggc acggcgcact tccaggtttc
     2101 cttcgaccac gtgccgccgt catggaacat gcagatgccc accgtgcagg tgtccgggct
     2161 gcaattcgca actacttccc ggtagcttct gagtgcgtac ctcattttta ctcgcgcgat
     2221 gcctctggct gccgatctgg agtccgctct gcgcgaagtt tgccgccgct ggcccggcgg
     2281 aactgcgcgg caattccgaa aagccgctgc tgcatctgtg gtcggaaaat cacaacctga
     2341 cgcggcgccg gctggttggc ggaaagctgg cgccgcgggc tgtcccgcca tcctgcgcca
     2401 ggaattgtcg gtatctttat cgcgacaacc tttgggccct tggcgggtta taatcgccat
     2461 gcggcacgaa aggttattcc tcgtgaattt ttcggtgaag atacgccagc aggaccacgt
     2521 cgctctgatt gatgtatgcg gccatttgac tttttttgaa gtacgcgcgc tgcgcgattc
     2581 cgtgcaatct ttgctgcgcg agaagcgcaa taacattctg ctgaatctga gcgagctgaa
     2641 atatctggac agctctggta tcggcgaact agcgcgcatc tatgtagccg tggtgaagca
     2701 aggcggcgcg atgaaagtgg ttggattggc gccaaaggtg gaagaaatcc tcaagatcac
     2761 ccatttgtcg caagtgttcc aggaatttcc cgacgagcag accgcactgc gcagctttgc
     2821 acagtaaatc cggccaatca acattctatt gatgctgtat ttgcagcttg acctccggcg
     2881 ctggcctgcg cgacactcgc attgaactgg cccctgcctg aacgacacta cccgccccaa
     2941 ctttcaatga aggaatctca tgcgcgagtc cctgctggca ttcctgcggt gccccgaatg
     3001 cggttgtacc ctgtcttcgc gacccagcaa gacacgtggc gaagagattt tggccggcga
     3061 cctagcctgt acgcaatgcg cggcaaggtt tgcagtacaa gccggagtgc cgcggatgct
     3121 gcgcgatgat gcacctgcaa cacgaaccca gctctccttt ggcaagcagt ggcaactgca
     3181 cagccaaggc agctttgagc ggggcccgat ttacagcaag agcaaagagg aatgtttgga
     3241 cgattttcgc aaagctttcg gactgcggga cttaagcggg cttcgcgatt gcgtcattct
     3301 cgatgccggt tgcggctcgg gtgagctgac cgcggacctc ggcgtcgctg caccctccgc
     3361 gacgatcatc ggaatggact tcagcgttgc ggcgcacgtg gcgtttgatc ggtgccgcgg
     3421 cattccgaac gtacacatcg tccaggcgga tttgtcgcgc ccgccatttt catcggaaac
     3481 gttcgacttc gtctggagcg agggcgtgat tcaccatacg ccggatacgg cgcggagttt
     3541 ctccagcctc tcgcacctgg tgaagtcgcg cgggaaactg tatgtgtgga tttattctga
     3601 tcgggtgacg acgccatacc ggctggcgcg caagattctg cgcaagccgt acctgctgcc
     3661 ttccgccgct ttatacgcgc tctcgtggag tctggcgctc ccgctgcact tgctgaacaa
     3721 aatgcgcgag atgctgcgac tttcgcgcgt gcggcaccgg ctctcgtcca cggcttactc
     3781 gttttacgac acgctctcgc cggagttcgt caactttcat tcccaggaag aagtgcatgg
     3841 ttggtttgcg cggcagcatt ttgacccggt ggtgtttcta accggcaccg acatcgcggc
     3901 ttgcggtaca aaaaaataga aaaaaaaaga ggcctaaaag gagagaagca gttgggattt
     3961 tggtcccaac tgcctcccgg ggtgtgtcct cgaagtggtt ttgttagttt agatgtatac
     4021 cagcgaaaaa ggtttcctaa aactaaaagt tttttgtgaa agtactgtta cggcaggaaa
     4081 ggcctagtaa aacaagtact tacgccaccg ctcgtcacga tggccgatca tccgcatgag
     4141 ctggcgagaa gtatgtagtg taaaaggtct aggccggcgc gccaatttga agccagcttc
     4201 ttccggcgtg cggtcgcctt tgctgttatt gcagcggtag cagcaggcta ccagattctc
     4261 ccaggacgac cgcccaccgc gagagcgcgg aacgacgtga tccagcgtaa gttctgaact
     4321 gggaaaaacc cgtccgcaga actggcaagt gttgcgatcg cgcagcaaaa tgtttttgcg
     4381 cgaaagtgcg cggctctgct gcgggatgtg ccggtaggcc agcaagcgaa tcaccgacgg
     4441 cactttgtgc gagttcgcag ccgaatgcac ttccgaaacg tgcagctctt ccgcctgggc
     4501 gacgcctttg agcaacagca ccatggcgcg gcgcacggcg gtaacgttaa tcggctcata
     4561 ggtggcgttg agcacgagca ccggctcctg cattagcgat gcacgacggg gcaccagcga
     4621 gttgtgaaat ctactgtctg cgggaacttc gctggaaggc acaaccgaac tggccgagtt
     4681 cgttggagcg aatcggcggg aacttacacc ctcttttcgc actgcgctca tcgcgcgtac
     4741 ctcaagccag ctctaacttt cgactttatg cgggttccga accaccgctc ggcctgccgt
     4801 gaagcagggc accctgctag cgcccgcgtc gcgcaacgct cccgcacacc catcgagcgt
     4861 cgcaccagtc gtcattacat gatctaccag taagacgcgc tagttgtcaa cttggctgcc
     4921 tggacgtgtg gcaaaagcac cacgaaacgg ccttgcgagc gatcgccttc cgataatgct
     4981 tggcagggcg gcgggcgccg tctgaccagc aaaatgtctt ggtgttcggc agttttagcc
     5041 gcttggcgcc cttttgatca gcctggttga agccccgttc ttggagaaac gggcggtctc
     5101 gtagccaagg atacgctcgc cagtgcctcg ccggcctcgg gtcagccagc gcgagatgcc
     5161 gcggcgcgtc gagatc
//
LOCUS       DASTPM010000075         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1357741, whole genome shotgun sequence.
ACCESSION   DASTPM010000075 DASTPM010000000
VERSION     DASTPM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357741"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2080)
                     /locus_tag="VFR42_01370"
     CDS             complement(<1..2080)
                     /locus_tag="VFR42_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU0367827.1"
                     /translation="MKLLSAPRQPFVGLALMAATGIIVAEIVPLTPAALILAAIFLAI
                     CILVALCWPKLAATYLVVAAGFFLLHKFATTNTAGQQLADKLGERPRVVTAVGYVITE
                     PKIAPSGFATFLLKLRSIEFEGKNESSRAVWQVRWKGAPEFGDELKLFGTAEPISPPR
                     NPGEFDMRAYLARHDVRRMLFARYPEDGTLIRHGGGNPVLRAAQASRTWMQHALCRGL
                     DNSPEVKSFISGIVLGIRHETPEDIEEPFQQTGTIHLFAVAGLHVGIVAALLWVLAAI
                     ARLSRKRAAAVIIPALFFYAAVTGLHIPALRAAVMASILVGSYFFERRVFLLNSLAAA
                     AFFILCWNTNQLFSTGFQLSFAVVGAIVLFADPFFRLFQRRAAPDPFLPQSLVRGPRR
                     WIHSSYEWLCGAASVSLAAWIGSLPLILWYFHLVTPISLLANLVVVPVAFFVLAIALL
                     SLMTTPLLPWVALIFNNANWSLATLVIGIVHLFAQIPGGHFYVGEPDWGDRISAKMTV
                     LDLGVGAAVHIRVNGHDWLIDCGSERSYERIVREYLHWAGVNRLTGLVLTHGDSQHIG
                     GVTQLLNDFPRVRVIDNPAPDRSVIHRRLSRIVAGLEGRRRKPAELAAGDNVDFSRDA
                     VAHVLFPPRGFAGATADDQALVVRLLIPRETFVLFMSDNGAETERALLGNGSDLLSGI
                     LVKGQHYSGIS"
     gene            complement(2168..>2489)
                     /locus_tag="VFR42_01375"
     CDS             complement(2168..>2489)
                     /locus_tag="VFR42_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367828.1"
                     /translation="AVTGTGGLFDCWLYLRWNGSLWFVFRLHAFMNLWWEIFGVAETA
                     LGGWIANAARLATIGLAILFTIYKGRLWKTDVPEVDWRSENRSYCSKLGAARSSSSVP
                     ALPQ"
BASE COUNT          628 a          733 c          694 g          434 t
ORIGIN      
        1 cggaaattcc ggagtaatgt tggcccttca cgaggatgcc actcagcagg tccgaaccat
       61 tgcccaacaa tgcgcgctcg gtttctgcgc cattgtctga catgaacagc acgaacgtct
      121 cccgcgggat tagtaaccgg acaaccaacg cttgatcgtc cgctgtagct cctgcaaacc
      181 cgcgcggcgg gaacaaaaca tgtgcaacag cgtcgcggga gaaatcaaca ttgtcgcccg
      241 cagccaactc agcaggtttg cgtctgcgtc cttccaggcc tgccactatt cgggacagcc
      301 gtcgatgaat gaccgaacga tcgggcgcag gattatcgat tacacggacc cgggggaaat
      361 cgttcagcaa ttgcgtgacg ccaccgatgt gctgcgaatc accatgggtg agcaccaacc
      421 cggtgagccg attcacgccg gcccagtgca aatactcacg cacgatgcgt tcgtaactgc
      481 gttcactccc gcaatcgatc aaccaatcgt ggccattcac tcggatgtga acggccgcgc
      541 ccacacccaa gtccagcacc gtcatctttg cagagatccg gtcgccccag tcaggttcac
      601 cgacataaaa gtgtccgccg ggaatttgcg caaacagatg aacgatgccg atgaccagcg
      661 tggccagcga ccagttggca ttattgaaaa taagagcgac ccacgggagg agcggcgtcg
      721 tcatcagcga cagaagcgca atggcgagga caaagaatgc gaccggaaca acgaccaggt
      781 ttgccagcaa agaaatcggt gtgaccaagt ggaaatacca aagaatcaac ggcaatgagc
      841 caatccacgc cgcaagcgac acagacgcgg caccacaaag ccattcatag ctggagtgga
      901 tccagcggcg tggaccgcgc accaaacttt gcggcagaaa gggatccgga gccgcgcgcc
      961 gctggaaaag tctgaagaac gggtcggcga acagcacaat tgcgccaacc accgcgaagg
     1021 aaagctggaa gccggtggaa aacagttggt tcgtgttcca gcagagaatg aaaaacgccg
     1081 cggccgcgag gctgttcagc agaaatactc gtcgttcgaa gaagtaactg cccacgagga
     1141 ttgaagccat cacagccgcg cgtaatgccg ggatgtgcag ccccgttacc gccgcataga
     1201 aaaagagcgc cggaatgatg actgccgctg cgcgcttgcg tgacaggcgt gcgatcgcag
     1261 ccagcaccca caaaagcgcc gccacaattc ccacatgcaa gccagcgacg gcgaaaaggt
     1321 gaatcgtgcc ggtttgctga aacggctcct caatgtcctc cggtgtttcg tgccggatgc
     1381 caagcacgat tccactgatg aaacttttta cctcgggcga gttgtccaag ccgcggcaca
     1441 acgcgtgttg catccatgtg cgcgacgctt gtgcagcgcg cagcaccgga ttgccgccgc
     1501 cgtgccggat cagcgtgccg tcctccggat agcgagcgaa cagcattcga cggacatcat
     1561 gccgcgcaag ataagcacgc atatcgaatt cgccggggtt gcgcggcggg ctgatcggct
     1621 cggcggtacc aaaaagtttc agttcatcgc caaactccgg cgcgcctttc caacggacct
     1681 gccagactgc gcgcgaggat tcgtttttgc cttcgaattc aattgatcga agcttgagca
     1741 ggaaggttgc gaatccgctc ggcgcaatct tcggctcagt gatcacatat ccgactgctg
     1801 tgacgacgcg cggccgctcg ccaagtttgt ccgcgagttg ctggccagcg gtgttcgttg
     1861 tcgcgaattt gtgcagcaaa aaaaaaccag ccgcgacgac gaggtatgtc gcagctaatt
     1921 tcggccagca gagcgcaacg agaatgcaga ttgcgagaaa tatcgctgct aaaataagcg
     1981 ctgcaggtgt cagcggaacg atctccgcca cgataattcc ggtcgcagcc atcaacgcta
     2041 gaccgacaaa tggctgacgc ggcgcgctca gcaatttcat tcaccgattt ttcgctgatc
     2101 gagcggtggc aaagcaagaa gcaatcgcgg ttagccttgc gaaggccgca tcagctctgc
     2161 aaacagatca ctggggcaag gccggtacac tggatgaact tcgggcagct ccaagctttg
     2221 agcagtagct acggttctct gagcgccagt cgacttctgg cacatcagtt ttccacaaac
     2281 ggcctttgta gatcgtgaag aggattgcaa ggccgatggt cgcaagccga gcggcgttcg
     2341 cgatccagcc acccagcgca gtctccgcga cgccgaatat ctcccaccaa agattcatga
     2401 acgcatgaag acggaacaca aaccaaagac tgccgttcca acgcaaatag agccagcaat
     2461 cgaacaaccc accagttcca gtgacagca
//
LOCUS       DASTPM010000076         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_781239, whole genome shotgun sequence.
ACCESSION   DASTPM010000076 DASTPM010000000
VERSION     DASTPM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781239"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VFR42_01380"
     CDS             <1..1105
                     /locus_tag="VFR42_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0367829.1"
                     /translation="DFESDPGDFPTATTDNRSSNYAGGQATLVYVQGRNNARAGIYGF
                     GQKDDELLGVLFNDGSNPDFRTPIDATGSLVALFAEEQFRATSWLTLNAGVRQTHFSG
                     HLVENATSPRFGVAVRVPRLNWTFRAFYGHFYQAPPLLTASGPVVQFVNSQNLSFIPL
                     RGERDEESQFGVTIPLRGWTFDADTFKTRAKNYFDHSNVGNSDIFFPLTVEGARIVGW
                     ELTIRSPKIKNRGQVYLTYSNQVAEGFGAVNGGLTDFSPPGGFFLLDHDQRNTLHFGG
                     TLNLPWRSYASTDVYYGSGFANGNAPPDHLPGHTTFDLTLGKDFGEKFSASLGATNVA
                     NRRVLLDNSFTFGGTHYLNPREIFAQVRYRFHY"
     gene            1143..1514
                     /gene="crcB"
                     /locus_tag="VFR42_01385"
     CDS             1143..1514
                     /gene="crcB"
                     /locus_tag="VFR42_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU0367830.1"
                     /translation="MRLTLIALFGAIGTLARYGLQGLIHVRVGGTFPYGTLLVNLSGC
                     FLLGLIGQLTLNRLVISPDWRVAIAVGFFGGYTTFSSFGWETAKMLEDGEWLPATTYV
                     SASVVIGLLLSVAGIRLANRF"
     gene            1525..1872
                     /locus_tag="VFR42_01390"
     CDS             1525..1872
                     /locus_tag="VFR42_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU0367831.1"
                     /translation="MPHEKFEGERTLMRIHIGESDKWHGKPLYEAIVEMLRKEGFSGV
                     TVLRGVAGYGGSSVYHTDKILRLSQDLPIILEIVETTERIEEILPRLDGMVEGGLITL
                     EKVRVILYRAAKK"
     gene            2006..>2376
                     /locus_tag="VFR42_01395"
     CDS             2006..>2376
                     /locus_tag="VFR42_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0367832.1"
                     /translation="MSESLAVGRAARPDLVSGDLAASRGSTAITRVGQVLDALAARDV
                     HGELLTAIRHFPAREAQWAEFPAWTHEDLRAAYASKSIRQLYSHQAAAAEAVHAGKNI
                     VVITPTASGKTLCYNLPIVNA"
BASE COUNT          511 a          591 c          755 g          519 t
ORIGIN      
        1 ggactttgag agcgatcccg gagattttcc gacggccaca acggacaatc ggtcgtcaaa
       61 ctatgctggc ggccaggcga cgctcgtata cgttcagggg cgcaataacg cgcgagcggg
      121 aatttatgga ttcgggcaga aagacgacga attgctcggg gttcttttca atgatgggag
      181 caatcctgat tttcgcacgc cgatagacgc caccgggagc ttggtggcgc tgtttgcgga
      241 agagcaattt cgggcgacct cgtggttaac gttgaacgcc ggggtgcggc aaacgcactt
      301 ttcgggacac ctggtggaaa acgcgacgag tccccgattt ggtgtagcgg tgcgcgtgcc
      361 gcggttgaat tggacatttc gagcattcta cggacacttc taccaggcgc cgccgctgct
      421 tacggcgtca ggtccggtgg tgcaatttgt gaacagccaa aatctgagtt ttattccgct
      481 gcggggagag cgggatgaag agtcgcaatt tggcgtcacg attccgctgc gcggctggac
      541 ctttgacgcg gatacgttca aaacacgcgc gaagaattac tttgaccaca gcaatgtcgg
      601 aaactccgac attttctttc cgctgacggt cgaaggagcg cgcattgtag ggtgggaact
      661 taccatccgg tctccgaaga taaagaatcg cgggcaagtg tatttgacgt actcgaacca
      721 ggttgcggaa ggctttggtg ctgtaaacgg cgggttgacg gacttttcgc cgccaggcgg
      781 ctttttcctg ctcgatcacg atcagaggaa cacattgcac tttgggggaa ctcttaacct
      841 gccgtggcgc agctatgcgt caaccgacgt gtactatggg tcgggatttg cgaatgggaa
      901 tgcgcctccg gaccatttgc ccggacacac gacgtttgat ttgacgctgg gaaaggattt
      961 cggagaaaag ttttcggcgt cgctgggggc gacgaacgtg gccaaccggc gggtgttgct
     1021 ggataacagc tttacatttg gcggcacgca ctacttgaat ccacgggaaa ttttcgccca
     1081 agtgcgatac cgtttccact actgattgag gatcaaggtt tggagcacag ggtgcggcac
     1141 acttgcgact gacactgata gcgctgttcg gagcgatcgg cacgttggcg cgttacgggt
     1201 tacagggact gatacatgtc cgcgtgggcg gaacctttcc gtacgggacg ctgctcgtta
     1261 acttgagcgg gtgttttctg ctggggttga tcgggcaatt gacgctcaat cggctggtga
     1321 tttctccaga ctggcgcgtg gcgattgccg tgggcttttt tggcgggtat acaacgttct
     1381 cgagctttgg ctgggagacg gcgaagatgc tggaggacgg agagtggctt ccagcgacga
     1441 cctacgtaag tgccagcgtt gtgattgggc tactgctgtc agtggcggga atccggctgg
     1501 cgaaccggtt ctaggagttt gcaaatgcca cacgaaaaat ttgaaggaga gcgcacgttg
     1561 atgcgcatcc atatcgggga atcggacaag tggcatggca agccgcttta tgaagcgatt
     1621 gtggaaatgc ttcgcaagga aggattctcc ggggtcaccg tgctgcgcgg ggtagcgggt
     1681 tacggcggtt cgagcgtata tcacaccgat aagattttgc gattgtcgca ggacttgccc
     1741 atcatcctgg aaattgtgga gaccacagaa cgcatcgagg agatcttgcc ccgactggac
     1801 ggcatggtcg aaggcggact gatcacgctg gagaaagtgc gcgttatcct ttaccgcgcg
     1861 gccaagaaat aggccgggcc atttcgatcc gcgattccct cgcagctcac caagttagaa
     1921 aaagcagacg agaacgctgt tgcacgccgg aaaacctcat gttagccttt ggcgaataaa
     1981 aggcgaataa ccggaggggc gagcaatgtc ggaatcattg gccgttggca gggcggcgag
     2041 gccagaccta gtttccggtg accttgcggc gagccgcgga tcgacagcaa tcactcgcgt
     2101 aggccaggtg ctggatgcgc tcgcggctcg cgatgttcac ggcgagctgc ttacggcgat
     2161 tcgacacttc cctgctcgcg aggcgcagtg ggcagagttc ccggcctgga cacacgagga
     2221 tttgcgtgcg gcctacgcca gcaagagcat tcgacaactt tacagtcatc aggcggccgc
     2281 cgcggaagcg gtgcacgccg gcaaaaatat tgtggttatc acgcccacgg cttcgggaaa
     2341 aacactttgc tacaacctgc caattgtgaa tgcgat
//
LOCUS       DASTPM010000077         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1271226, whole genome shotgun sequence.
ACCESSION   DASTPM010000077 DASTPM010000000
VERSION     DASTPM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271226"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1460
                     /locus_tag="VFR42_01400"
     CDS             105..1460
                     /locus_tag="VFR42_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEU0367833.1"
                     /translation="MKKRTFLQLSGAMGAQSMLSPLLGWGARVPEKLKNWAGNIEYST
                     ESLYAAKSVEEVKSYVATTSKLKVLGTRHCFNTIADSKDAFLSLKPMEDVVALDAAAK
                     TVTLHAGVTYGRLAPYLHERGFALHNLASLPHISVVGACSTGTHGSGQKNGNLSTAAS
                     GLEMVTASGDVVVLARKKDGDKFNGAVVGLGALGAITKVTLDIQPTFMVRQWVYENMP
                     FEAATAHFDEIEASGYSVSLFTDWRNKNVNEVWIKERVGGGKEFAAKPEFFGAKLAKK
                     NLHPIAELGAENCTEQLGVPGPWYKRLPHFKMGFTPSAGKELQSEFFVPRKHAVEAIL
                     AVEKLHEKISPHLLITEIRAIAADDLWMSPCYQQDSLAIHFTWQQDWPAVKTVLPDIE
                     KTLSPFGVRPHWGKLFTMEPAVLRSRYKRLGEFVELAKKYDPQGKFRNEFVNAYIFGN
                     R"
     gene            complement(1583..2230)
                     /locus_tag="VFR42_01405"
     CDS             complement(1583..2230)
                     /locus_tag="VFR42_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367834.1"
                     /translation="MLRSTLIFLTVSVFLGQSAPERKVAGSAVSSTHDPHVQIHLPES
                     AQYVGADRWILYGIADCELHAFVEADAQHNVQRLYWVQFEGYVPSKPELTHTYDSARR
                     TAIGGLDFYVDTWPRADGEPTKPGSDREHIETLIRSKGYKMPAGMMYVRLVHLLDAKK
                     RKELMIIYGEDLAPTGFSAADLVQGGKSHHRWPELENGLIERAKVRIALEPAASR"
     gene            complement(2295..2687)
                     /locus_tag="VFR42_01410"
     CDS             complement(2295..2687)
                     /locus_tag="VFR42_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367835.1"
                     /translation="MKGFVGLLVALLTAGGVYFFYMKRMPTADAGTAPTQAISLTGVR
                     MDLMQIAQAERAYLASNGRCGSLGDLTSSGTLNLARTERDGYFYEVKCGDGSDFAVIA
                     HHAPAPPDSPIRYPILAIDQNLQVGEVQ"
     gene            2882..>3537
                     /locus_tag="VFR42_01415"
     CDS             2882..>3537
                     /locus_tag="VFR42_01415"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEU0367836.1"
                     /translation="MKMKSPYACLAAMAMSALLCVPNEIAAADTPGQRAGEVSRVIPT
                     VNIARGAKSFNASAKTQVDWLDVVNTQASARARITLDDGSVLNVGSESSIKVVKHDGG
                     AQQTELELTYGKLRSQAQKITKPDGKFQVKTPAGVAGVVGTDFFVSFDNTTGTMNVTV
                     FEGMVKVCNLAGVCVEVKAGQFSNLRTNDSTGPQPPTQATLDVLMSAGSTTDVGGGPG
                     V"
BASE COUNT          806 a          846 c         1198 g          687 t
ORIGIN      
        1 atgggataag tagcgccaag agatatacaa tcttggcgtt tttaagctat ggatggattt
       61 cgccattcct tcgcggaaga ggccggcgga gcggcttatc acgcatgaaa aagagaacct
      121 ttttgcagtt aagtggagca atgggcgcac aatcgatgct ttctcctctg ttggggtggg
      181 gagccagagt gccggaaaag ctgaaaaatt gggcgggaaa tatcgagtac agcacggaaa
      241 gcttgtacgc ggcgaaatct gtcgaagagg tgaagagtta cgtcgcgaca acgagcaagc
      301 tcaaagtact gggcacgcgg cactgcttca acaccattgc agacagcaag gacgcttttt
      361 tgtcgctgaa gccgatggaa gacgtggtgg cgctggatgc ggcggcaaaa acagtgacgc
      421 tgcatgcggg cgtgacgtac gggcggctgg cgccgtacct gcatgaaaga ggatttgcgc
      481 tgcacaacct ggcttcgctg ccgcacattt cggtggtggg agcgtgcagc acgggaacac
      541 atgggtcggg ccagaagaac ggcaatttgt ccacggcggc cagcgggctg gagatggtga
      601 cggcgagcgg cgatgtcgta gtgctggcgc gaaaaaagga cggcgacaaa ttcaacggag
      661 ccgtggtggg tttgggcgcg ctcggcgcaa ttacgaaagt gacgctggat attcagccga
      721 cgttcatggt gcggcagtgg gtttacgaaa acatgccgtt cgaggcggcg actgcacatt
      781 ttgacgaaat cgaagcgagc ggttacagcg tgagtctatt cacggactgg cgaaataaga
      841 acgtgaacga agtgtggatt aaggaacgcg tgggcggcgg aaaagagttc gcggccaagc
      901 cggaattttt tggcgcgaag ctggcgaaga aaaacctgca tcccatcgcg gagctgggtg
      961 cggagaattg cacggagcag ctgggtgtgc cggggccgtg gtacaaacgg ctgccgcatt
     1021 tcaagatggg attcacgccg agcgcgggga aagagctgca atcggaattt tttgtgccgc
     1081 gcaaacacgc ggtagaagca attttggcag tggagaaatt gcatgaaaag atcagcccgc
     1141 acttgctgat cacagagatt cgggcaattg cggcggacga tttgtggatg agtccatgtt
     1201 atcagcagga ttcgctggcg attcatttta cgtggcagca ggactggcca gcggtcaaga
     1261 ctgtgctgcc ggatattgaa aagacgttgt cgccgtttgg tgtgcggccg cattggggga
     1321 agctgtttac gatggagccg gcggtgttgc ggtcgcgcta caagaggtta ggggagttcg
     1381 tggaactggc gaagaaatat gacccgcagg gaaagttccg gaacgaattc gtgaacgctt
     1441 atatttttgg caatcgatga acgccaaacg agcaagagag attgcggtac agacgatact
     1501 ggcatttttt ctggttgtct tgcttgtctc cttgctggcg aaaagattgt ttcatcccag
     1561 ataaattcgc ttcgaccacg tttcagcgcg atgctgcggg ctcgagcgcg atcctgacct
     1621 tggcacgttc aatcaagccg ttttcgagct ccggccaccg gtggtgtgat ttgccgcctt
     1681 gcacaagatc ggcggcgcta aaaccggtgg gcgccagatc ttcgccgtag atgatcatca
     1741 actccttgcg cttcttggcg tcgagcagat ggacgaggcg gacgtacatc ataccggcgg
     1801 gcattttgta gcctttactg cggatgagag tttcgatgtg ttcgcggtcg gaaccgggct
     1861 tggttggttc gccgtcggcg cgcggccagg tgtccacgta gaaatcgagt ccgccgatgg
     1921 cagtgcggcg cgcagaatcg taagtgtggg tgagttccgg cttggatggg acgtagcctt
     1981 caaactgaac ccaatacaga cgttgcacgt tgtgctgcgc atcggcttcg acaaaggcgt
     2041 gcagttcgca gtcggcgatg ccgtaaagga tccagcggtc cgcgcccacg tattgggcgg
     2101 attcgggcag atggatttgc acgtggggat cgtgcgtgga agaaacagcc gagccggcga
     2161 ctttgcgctc gggcgcggat tggccgagga acacgcttac ggttagaaag atcagagtgg
     2221 atcgcagcat aggtggccgc cgacaaaaat agtgccacgc ctaggcgtat aaagtgcagg
     2281 aaatcagcga aaggctactg gacttcgccg acttgcaggt tctgatcaat agcgagaatg
     2341 gggtagcgaa tcggagagtc tggcggagct ggggcgtgat gagcgataac ggcaaaatcg
     2401 gagccgtcgc cgcatttgac ttcgtagaag tagccgtcgc gctcggtgcg agcgagattc
     2461 agggtgccag acgaagtgag gtcgccgagg gagccgcagc ggccgttaga agccaagtaa
     2521 gcacgctcgg cttgcgcgat ttgcatcagg tccatgcgaa cgccagtcag gctaatcgct
     2581 tgcgtgggcg ctgtaccagc gtccgcagtg ggcatcctct tcatataaaa aaaatagacg
     2641 ccgccggcgg tcagcagagc cacgagcaat ccgacaaagc ctttcatgag agcagtgtgg
     2701 cgcgaggcct ggcgccaggc aagtggccgg aagttcaggc gtgcggcgga aggcaacgtg
     2761 tcggggaatg cgcggccatg gcattggcag acgagcaacg ccaacgggaa ttcaaattga
     2821 cggcgcgggg cgtccggtaa caacaatagg taagccgaaa gtgtccttag gagagttccc
     2881 aatgaagatg aagtccccgt acgcctgcct ggcggccatg gcgatgtcgg ccctgctgtg
     2941 cgttcccaat gagattgcgg cggccgacac gcccgggcag cgagcgggcg aagtctcgcg
     3001 agtgattccc accgtgaata ttgcgcgggg cgcgaaaagc ttcaatgctt ccgccaagac
     3061 gcaggtggat tggctggacg tggtgaacac gcaggctagc gctcgggcgc gcattacgct
     3121 ggatgacggg tcggtgctga acgtggggtc ggaatcttcg atcaaagtgg tgaagcacga
     3181 cggcggagcg caacaaacgg agctggagct gacctatggc aagctgcgtt cgcaggccca
     3241 gaagatcacc aagccggacg gaaaattcca ggtgaagacg ccggccggag tggcgggcgt
     3301 ggtgggtacg gatttctttg tgagcttcga caacaccacg ggaaccatga acgtgaccgt
     3361 gttcgaaggt atggtgaagg tttgcaattt ggctggcgta tgtgtggaag tgaaggccgg
     3421 ccaattctcc aacctgcgca ccaatgacag cacaggcccg cagccgccga cgcaagcaac
     3481 gctggacgtg ctgatgagcg cgggatcgac cacggatgta ggcggcggac cgggcgt
//
LOCUS       DASTPM010000078         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_2740, whole genome shotgun sequence.
ACCESSION   DASTPM010000078 DASTPM010000000
VERSION     DASTPM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFR42_01420"
     CDS             <1..266
                     /locus_tag="VFR42_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEU0367837.1"
                     /translation="AMIPGLLEYLDYPDIATLAMPRPLLVVHGQQDTLFPPEGVKAAM
                     DTLRKSYQTIGQPERFSTFIFDGPHKFPFTAQQKMMDWFDRWV"
     gene            775..2520
                     /locus_tag="VFR42_01425"
                     /pseudo
     CDS             775..2520
                     /locus_tag="VFR42_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
     gene            2728..2970
                     /locus_tag="VFR42_01430"
     CDS             2728..2970
                     /locus_tag="VFR42_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0367838.1"
                     /translation="MPMLENTTQEVLALAEQHFKLAPGSLSANDDFFKKLGIDSLQTL
                     ELLSRLENHFNIELPDYEVQGVSDFKTLAERIQSRL"
     gene            2970..>4640
                     /locus_tag="VFR42_01435"
     CDS             2970..>4640
                     /locus_tag="VFR42_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0367839.1"
                     /translation="MLDLPKYSSIGAALKDAVEKYANETCLIESDREREKERITYQEF
                     GARSLPLARALQDAGFSAADRASIIMTNQSKWLISAYAIFYAGGVLVPLDYKLTPAEH
                     WQLLQHSGTKILITEYPIWRQLSTSPERANAANLQTVLVTEAPPKVDLAGALRWEDFK
                     SEQEPTFVPRDRKDVASIVYSSGTGGRPKGCMMTHENYLEQCVALTSLYPFWPGVRYL
                     SILPTNHAIDFMVGFFGPFTCGASVVHLRTLRPEFVRQAFTKYKITYVSLVPLVLKNL
                     QKGLQAKFAELPPSKRKIFDKLVAVNKAFTKSHPHLWLSRLLLKQVHAAFGGELRAII
                     VGGAFTEPQTLQLFYDLGIPVANGYGLSEAGTAITVNDLKPFRADTVGKPLPGMEVKI
                     VDPASDGVGEVYVRSKTVMSGYLNDPELTAETIVDGWLRTGDLGQFDARGHLHLSGRK
                     KNMIVTEEGKNIYPEDIESVFESLPVKEFCVFAANYVWPKRSMVGEQLILAIHLENNQ
                     FFTEQLKNDIAKRNSRLLNYKRIHGMVLFNEDFPRTASLKIKRNDLAIL"
BASE COUNT         1061 a         1396 c         1200 g          983 t
ORIGIN      
        1 cagcaatgat tcccggactg cttgagtatc tggactatcc ggacatcgcg acgctggcca
       61 tgccccgccc gctcctcgtg gtccacgggc agcaggacac gctgtttcct ccggaaggag
      121 tcaaggcggc catggacacg ttgcgcaagt catatcagac catcggccag ccggagcggt
      181 tcagcacgtt tatttttgac ggtccccaca agtttccgtt cacggctcag caaaaaatga
      241 tggactggtt cgatcggtgg gtttagggaa atgcggtagg aacgccttac ggtctcctga
      301 tgcgccgttt gccgggccgc gtagccgcga taagcaaaag ccctgggcgc aatgtcgcgc
      361 ggcagaatcg actccaggtt tggcgacgga cacaacccgc cgaatatccg aattcctcat
      421 ttcgcaagtg gtcgaaaaga tggtgagtgg caggctcgtg gggactcgaa gcgcagatcc
      481 accgtgttta gcgtatctcc attgcgcacg caactgcacc agcacgcata tgcgcttcca
      541 tgcgaagaga cctggggtta tggcacaaag tacggcacaa attttcgccg ccctctgcgt
      601 cgccgggaag ttggtgtcct agccgaactt tcgcagttgc cgacgttttt cggttttttg
      661 ctctgatttt tattgttaag ccgtgtgttt tcatcgttgc tttctgaaaa aacggtagag
      721 tgaagaccaa gtctcttccc ttggacaccg gagttctggc agtctgcggt cagcatgttt
      781 ctgcgcagca ttcatcgcaa aaaagacggc aaggatcatc gctacttcag cattgtagaa
      841 aaccatcgca tcgcttccga caagacggtg cagcgaaccg ttctctatct cggtgagatt
      901 aacgatcaac aacaagcgac atggcgcaaa acgctgtcgg tgtttgacga tgagcagcaa
      961 gagtatgcga acttgagttt gttccccgac gatcgtgaga ttcccgccga tgcggtcgac
     1021 agcctgcaag tgaaactgag cggcctcgaa ctgcggcgcc cgcgtctgtt cggtagctgc
     1081 tggctgggat gtcagttgtg gcagcaactg ggcctggatg agttttggga agctggcctt
     1141 gccggatcgc gcgaagacgt cgcttgggaa aaagtattgc aactactggt ggtgaaccga
     1201 ctgctggatc caggcagcga atttcgtgtg catcgccagt ggtacctgac caccgccatg
     1261 gatgcattgc tgggaaccga tttcgcggtg gccgagaagg atcgcttata tcgctgtctc
     1321 gaccgggtgt tggaacacaa gcaggaactc tttctatggc tgcgccagaa gtgggccgat
     1381 ctgtttcagg ccgacttcga agtgctgctc tacgacctga ccagcacgta cttcgagggc
     1441 gcgatggaag agaaccccaa ggccaagtac ggtcacagtc gcgataaacg gacggattgt
     1501 ttgcaggtgg tgattgcgct ggtgatcacc agcgatggtt ttccgttggc ctacgaagtg
     1561 atggacggca acaccagtga tcgcaccaca ctgcgtgggt ttctggacca catcgagaag
     1621 acctatggaa aagcaaaacg cctgtgggtg atggaccgcg gcattccgac cgaagagatt
     1681 ttgcaggaga tgcgggatcc ggcacgcgag attttctatc tggtgggaac accgaaaggc
     1741 aaaatccaac agtatgaaaa gaagtggctg gatttgccct ggcagaaggt ccgagattca
     1801 gtggacgtga aactgtttga gcaggacggc gaactctatg tgctggccaa gagtgaagga
     1861 agaagggcca aagagtccgc catgcgccgc aaacgtctgg cccgcttgct cagaaagctc
     1921 cgtgccatgc ggcgcagctt gccttctctg gtccagttgt tgatgcgctt gggcgccgcg
     1981 aaagcagcag cgggccgcgc attccagttc gtgcagatgc aaatacccag ggaaggccag
     2041 caggtcacgc gggagacttt tcagtttcgt gtggataaga aaaaactcca agaggcggaa
     2101 tggcgcgacg gacattactt gctgcgctcc aaccttaccg caggagatcc cagtgtgctg
     2161 tggatgcgct acgtgcagct gacccagatc gaatcggtct tccgatctct caagagcgaa
     2221 ctcggcatac gccccatcta tcatcaattg gagcatcgcg tcgacgcgca cattctgatc
     2281 gcttttctcg cttactgttt gcaagtgacg ctaaagcatc gcctgttgct gcacgccccg
     2341 gggttgacac caacggcggt gctggagaag ttggccgaca tcaaaatgat cgatgtttgg
     2401 attccgaccg tcgatcaacg gtggttgatt ctgccgcgct atactcagcc ttctccagac
     2461 atcaagctac tcatcgagaa gctgaaattg catttgccca gccaaccgcc accgcgcctt
     2521 gttgcgcgaa aagagagctc cgacatcgaa gtacaaaccg tccggcagga gggccgtttg
     2581 tggtgaagac ctttcactga cctcccttga ttccaaagca cttaactggc tcagaggtgc
     2641 ccaactgcga aagtccggct aactacatcc actccagaaa cgccgcattt ttcttctcgt
     2701 gtcaggggtg ttaaattatt cctagtcatg cccatgctcg aaaacactac ccaggaagtc
     2761 ctcgcgctcg ccgagcagca cttcaagctc gcgcccggct cgctctctgc caacgacgac
     2821 ttcttcaaga aacttggtat cgactccctg caaacgctcg aactgctctc ccgcctcgaa
     2881 aatcacttca acatcgaact acccgactac gaagtccagg gtgtctccga cttcaaaacc
     2941 ctagccgaac gcatccaatc ccgcctgtaa tgctcgatct cccgaagtat tcttccatag
     3001 gtgccgcgct gaaagacgcg gtagaaaagt atgccaatga aacttgtctg atcgaatccg
     3061 accgtgaacg agaaaaggag cgcatcacgt atcaagagtt tggcgcacgc tccctcccgc
     3121 tcgcccgcgc cttgcaggat gccggattct ccgccgccga ccgtgccagc atcataatga
     3181 ccaatcagtc caagtggctg atctccgcct atgcgatttt ctacgctggc ggcgtcctcg
     3241 ttcccctcga ttacaaacta actccagccg agcactggca gctcttgcaa cactccggca
     3301 caaaaattct gatcaccgaa tatcccatct ggcgccagct ttccacttcg cccgagcgcg
     3361 ccaacgccgc aaatctgcaa accgtcctcg tcaccgaggc tccgcccaag gtcgatctcg
     3421 ccggcgccct tcgctgggaa gacttcaaat ccgaacagga acctaccttc gtcccgcgcg
     3481 accgcaaaga cgtcgcctcg atcgtctact cctccggcac cggcggccgc cccaagggct
     3541 gcatgatgac ccacgaaaat tatctggaac agtgcgtcgc ccttacttcg ctctatcctt
     3601 tctggcccgg cgtgcgctat ctcagcatcc tccccaccaa tcacgcgatc gatttcatgg
     3661 ttggcttctt tgggccgttc acctgcggcg cctctgtcgt gcatctacgc acgctacgcc
     3721 ccgaattcgt tcgccaagcc ttcacgaaat acaaaatcac ctacgtcagt ctcgtcccgc
     3781 tagtcctgaa aaatctgcaa aaaggtctgc aagcaaaatt cgccgaactc cctcccagca
     3841 aacgcaaaat cttcgacaag ctcgttgctg tcaataaagc cttcaccaaa tcccatccgc
     3901 acctctggct aagccgtctc ctcctgaagc aggtccacgc agctttcggt ggcgaactcc
     3961 gcgccatcat tgtcggcggc gctttcaccg aaccgcaaac cctgcagttg ttctatgacc
     4021 tcggcatccc cgtcgccaac ggctatggcc tcagcgaagc cggcaccgcc atcaccgtca
     4081 atgacctaaa gcctttccgt gccgacaccg tcggcaaacc gctgcctggc atggaagtca
     4141 aaatcgtaga tcccgcttcg gacggcgtcg gcgaagtcta cgtccgcagc aaaacagtca
     4201 tgtccggcta tctcaacgat cccgaactca ccgccgaaac catcgtggac ggctggcttc
     4261 gcaccggaga tctcggccag ttcgatgcgc gtggccatct ccacctcagc ggccgcaaaa
     4321 aaaacatgat cgtcaccgag gaagggaaga acatctaccc ggaagacatc gagtccgtgt
     4381 tcgaatcgct gccggtgaag gaattctgcg ttttcgccgc aaactacgtc tggcccaaac
     4441 gttccatggt tggcgagcag ctcatcctcg ccatccatct cgagaacaac cagttcttca
     4501 ccgagcaact gaaaaacgac atcgccaagc gcaacagccg cttgctcaac tacaaacgca
     4561 tccacggtat ggtcctgttc aacgaagact ttccccgcac cgcctccctg aaaatcaaaa
     4621 ggaacgacct cgctatactc
//
LOCUS       DASTPM010000079         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1184795, whole genome shotgun sequence.
ACCESSION   DASTPM010000079 DASTPM010000000
VERSION     DASTPM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184795"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VFR42_01440"
     CDS             complement(<1..300)
                     /locus_tag="VFR42_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367840.1"
                     /translation="MLTAPGAFAAGAALFLIVMWDAFEAIILPRRVTRKFRLTRLFYR
                     STWRVWKLLVNLIPVRKTREALLGFYGPMSLILLIAVWAVGLVFGFGLMQFGTGSA"
     gene            complement(335..760)
                     /locus_tag="VFR42_01445"
     CDS             complement(335..760)
                     /locus_tag="VFR42_01445"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0367841.1"
                     /translation="MQRKASAVWQGDLKTGKGAISTDSGVLKQTQYSVSTRFENGVGT
                     NPEELLAAAHAGCFTMALSAQLGNAGLTAQKLETTATVSLEKVGDSFSITKSHLDLVA
                     RVPGADKAKFDAAVRAAETGCPVSKLFKAEISVSARLES"
     gene            881..1627
                     /locus_tag="VFR42_01450"
     CDS             881..1627
                     /locus_tag="VFR42_01450"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEU0367842.1"
                     /translation="MTSILDCTAHRPWPLPSGPWIMAQSWHDLLFAHWPIDAGALSRL
                     VPAELEIDTFEGSAWLGIVPFRMSGVRWRWTPALPWLSAFPELNVRTYVTAQGKPGVW
                     FFSLDATNAMAVAVARMSFHLPYFRARMACREVDGWIEYRSRRTHRGALDAVFEARYQ
                     GTGEVFEPKRGSLEHFLTERYCLYSAVSSGRIYRGEIHHPPWALQKAEAQFTKNTMAS
                     GAGLTQPDGLPLLHFARRQTMVAWAPRRIK"
     gene            complement(1635..1964)
                     /locus_tag="VFR42_01455"
     CDS             complement(1635..1964)
                     /locus_tag="VFR42_01455"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEU0367843.1"
                     /translation="MRTILLLTISNIFMTFAWYGHLKYRSEALWKVILASWGIAFFEY
                     CFQVPANRIGSYEFTTAQLKTIQEVITLSVFAVFSVFYLGEKLKWNYLAGFGCITLAV
                     FFVFHKW"
     gene            2161..>2595
                     /locus_tag="VFR42_01460"
     CDS             2161..>2595
                     /locus_tag="VFR42_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0367844.1"
                     /translation="MNLRARLRARIFGFLLASLIGSGGLLAQAQTSITLVATGSSLPE
                     PLYVAWGDAYHTRHPETQMRYLPEGTGESARKILSGVGDLGGGDAPVPENSMKGATTP
                     VLELPTVVIGIAIVYNLPSTAGELRLSGQVLADIFLGKVKIWN"
BASE COUNT          545 a          702 c          770 g          578 t
ORIGIN      
        1 cgccgagccg gtaccaaatt gcatcaagcc aaagccgaat accagaccga ccgcccaaac
       61 cgcaatcagc aaaatcagcg acatcgggcc gtaaaaaccg agcaaggctt cgcgggtttt
      121 gcgaacggga atgagattta cgaggagctt ccaaacgcgc caggtgcttc gataaaacaa
      181 gcgggtcagg cgaaactttc gcgtgacgcg gcgcggcagg atgatggctt cgaacgcatc
      241 ccacatcaca atcaggaaca gagccgcgcc tgcagcgaac gcgcccgggg ctgtgagcat
      301 tgggggcatt ttgcttatcc gccgaacggg tggatcaaga ttccaaacgc gcgctgaccg
      361 aaatttccgc tttgaagagc ttggaaaccg ggcagccggt ttcggcggct cttactgcgg
      421 cgtcaaactt cgctttgtcg gctccgggaa cgcgggcaac caggtcgaga tggctcttgg
      481 tgatgctgaa gctgtcgccg actttctcga gacttacggt tgcggtggtt tccagtttct
      541 gcgcggtcag gccggcattg cccaactgcg ctgaaagcgc catagtgaag cacccggcat
      601 gcgcggccgc gagcagttct tcgggattgg tgcccacgcc attttcgaaa cgggtgctga
      661 cggaatattg cgtttgcttg aggacgccgc tgtcggtgga aatggcgcct ttgccggtct
      721 tgagatcgcc ttgccagacc gcacttgctt tacgttgcat actttctcct tgtcgctttg
      781 aagcttttga aagttttgaa ttccagagat tcgattcgtc tacgccttcg aagggtatat
      841 tgaacggggc gaaggtgtcg agagcacgaa tcctatatcg atgacttcca tcttggattg
      901 caccgcgcac cggccatggc cgttgccgtc gggcccatgg attatggcgc aatcgtggca
      961 cgacctgttg ttcgcgcatt ggccgattga cgcgggcgcg ctgagccggc tcgttcccgc
     1021 ggagctggag atcgacacgt ttgagggcag tgcgtggttg ggcattgtgc cgttccgcat
     1081 gtccggcgtg cgctggcgct ggacgccggc gctgccgtgg ctttcggctt ttccggaact
     1141 gaatgtgcgg acctacgtca ccgcgcaggg taagcctggc gtttggtttt tcagtctcga
     1201 tgcaacaaac gccatggcgg tggcggttgc caggatgtcc tttcacttgc cgtattttcg
     1261 ggcgcggatg gcgtgccgtg aagttgatgg atggattgag tatcgcagcc ggcgaacgca
     1321 tcgaggcgcg ctcgacgctg tttttgaagc gcgataccaa ggcactggcg aggtcttcga
     1381 gccaaaacgg ggctcgttgg agcattttct gacggagcgt tattgcttat attcggcggt
     1441 ctccagtggg cgaatttatc gcggcgaaat tcaccacccg ccatgggcct tgcaaaaggc
     1501 cgaggcacaa ttcacgaaaa acaccatggc gtccggtgca ggtctcactc aacccgacgg
     1561 gcttccgctg ctgcactttg cgcgccggca aactatggtc gcctgggcgc ctcgccgaat
     1621 aaaatgacca cctctcacca tttgtgaaac acaaagaaaa cggccagggt gatgcagcca
     1681 aatcccgcca aataattcca cttcaacttt tcaccgaggt agaagaccga aaatacagcg
     1741 aatacggaca gcgtgatcac ttcctgaatg gttttgagtt gtgcggtcgt gaattcgtac
     1801 gagccgatgc gattggccgg aacctggaag cagtattcaa agaaagcgat gccccagctg
     1861 gccaggatga ctttccacaa agcttcgctg cgatatttga ggtggccata ccaggcaaag
     1921 gtcataaaga tgtttgagat ggtcagcagc aggatggttc gcacaggtat ctccgacagt
     1981 tgatagtgtc gcttctcaga ggcagtgtat tcgggaacgg tcgcgggtca aagtcgcgag
     2041 tcgaaatcga gttgctgcat gagtgccagt ttcggcaccg cgaagcaact ttctcgctcc
     2101 gcttcgctcc aacggatcta gcgtagctac gttcgtcaat attccctctc ggagaacacg
     2161 gtgaacctac gcgctcgttt acgtgcacga atatttggtt tcttgctcgc gagcctgatt
     2221 ggaagcggtg ggctccttgc ccaagcacaa acttcgatca ctttggtggc aactggttcc
     2281 agcttgcccg aacctcttta cgtagcgtgg ggcgacgcct atcacacccg gcatccggaa
     2341 acgcagatgc gttacttgcc cgaaggcact ggcgagagcg cgcgcaagat cctttccggc
     2401 gtaggagatt tgggcggcgg cgacgcgccc gttccggaga acagcatgaa gggagccacc
     2461 acacccgtat tggaattgcc cactgtggtg atcggcatcg ccattgtcta taacttgccg
     2521 tcgacggccg gtgaattgcg tctttccggg caagtacttg cggacatttt cttgggcaaa
     2581 gtgaaaatat ggaac
//
LOCUS       DASTPM010000080         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1213563, whole genome shotgun sequence.
ACCESSION   DASTPM010000080 DASTPM010000000
VERSION     DASTPM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213563"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..2726)
                     /locus_tag="VFR42_01465"
     CDS             complement(66..2726)
                     /locus_tag="VFR42_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0367845.1"
                     /translation="MTDQIRILATRFRALFRRRSLDRELDADLRSHLDMAVEHNLRQG
                     MSPEQARREALLGFGGLEQTKEIYREHRGLPFLETFLQDLRFALRMLRKSPGFTAIAV
                     LTLAFGIGTNTAIFSMVNALLLHPYRFHDLESLVLIWENRGIDEGFDARWISPGDTSD
                     LRANLQLFEDVASYQCGDFNLSSEGRVDMARGCRVSANFFSVLGVGPTQGRRFSAEEE
                     QPGSDQSVIISNSFWQSRFTGDPQILGKTIRLNARNYTIAGIMPPDFDFPVPMELWVP
                     LALTPAEKSDRAQLSLAALGRLKPGITVREAAAALSNLSQRLAELYPNTNAGRVLNIL
                     QLRKELYLFTLPLFLLLQGAAAFVLLLACANFANLLFTRIITRQKELAMRSALGASRT
                     RLARLLVSEMSLLAMLGGAMAIAASFSSVRILRNSISPDWTKWVPGWEGIQVDSTVLS
                     FAILLSLLLVLVFGLSTALPLRDIDLNTVLKETGRSSSGPSRGYLRNGLVSTQVALAL
                     VLLVCAGLTMQGFNRIAHVYQGFQPDQVLKFEVALPETSYPEGAKCAAFFQTALRSVS
                     SLPGVSGAALTSNLPASNVDNEKTLFTIEGRPALQASETPAADLQIISPDYFSVLKIP
                     LVAGRLFDEADAASAARVVLISRTMATRFWPAGDPLRQKLKVGAADSAEPWMTVVGVV
                     GDVRQNWWNPAAFPVIYQPYLQSSRRSFRFALRVASDPAGYAPAARAAISQIDPEIPI
                     TELKTLQSEVQDSIAIVHIMGILMGVFGAVALVLSSIGVYGILSENVAQRSHEFGIRF
                     ALGANPRDVLRLVLRHALIVSGVGLAVGLPIAFAVSRAMAAFVFGIVSVSLAVVAVLA
                     GLLILVALAAAYFPARRALRVDPMVALRNE"
BASE COUNT          686 a          768 c          864 g          502 t
ORIGIN      
        1 cggacaaacg ctacgccgtc tcgagcccat tttcatcttc gttgtgggcc cgcagggacc
       61 atgccttact cgtttcgaag cgccaccatg ggatcgacgc gaagcgcgcg gcgcgcgggg
      121 aaataggcgg ccgcgagcgc aacgagaatg agcagtcccg cgagcaccgc cacaaccgcc
      181 agactcaccg agacaattcc aaagacaaat gcagccatag ctctgctcac cgcgaacgca
      241 attggcaagc caacagccaa accgacgccg gaaacgatca gcgcatggcg caagacgagg
      301 cgaagcacat cgcgcggatt cgctccgagg gcgaaccgga tgccgaattc gtggctccgc
      361 tgtgcaacat tctcggacag aattccgtaa actccgatgg aggaaagcac caaggcgacg
      421 gctccgaaaa cgcccatcag aatacccatg atgtgcacga tggcgatgga atcctgaact
      481 tcggactgca gggttttcag ctcagtgatt ggaatttccg gatcgatttg agaaatcgcc
      541 gccctggccg ccggggcgta accggctggg tccgatgcca cgcgcaaagc gaaacggaat
      601 gagcgccgtg aagactggag ataaggctga tagatgacgg gaaaagctgc agggttccac
      661 caattctgtc ggacatctcc gacaacgcca accacggtca tccaaggttc agcagagtct
      721 gcggcaccga ccttgagctt ttgtctcagc ggatcgccag cgggccaaaa gcgagtggcc
      781 atcgtccggc tgataaggac aacgcgagcg gcactcgcgg cgtctgcttc gtcgaacaat
      841 ctcccggcaa ccagaggaat cttgagaacg gagaaataat ccgggctgat aatttgcaag
      901 tcagcggcag gcgtttcgct ggcttgcaga gcggggcggc cttcgatggt aaacaatgtc
      961 ttttcattgt ccacgttgct ggcgggaaga ttggacgtta aagcagcgcc agaaacaccc
     1021 ggcagggaag aaacactacg caacgcggtt tgaaagaaag cggcgcattt tgcaccttcg
     1081 ggataggaag tctccggtaa agcgacttca aatttcaaaa cttgatctgg ttgaaagcct
     1141 tgatagacat gcgcaatccg gttgaaaccc tgcatggtca aacccgcgca gaccaaaaga
     1201 actagagcga gggctacctg agtggaaacg agcccgttcc gcaggtaccc gcgagacggt
     1261 ccggaggaac tgcgtcccgt ttccttgagc acggtgttca ggtcgatatc gcgtagcggg
     1321 agcgcggtcg agaggccaaa gacgagtacc aggagcagtg aaagaagaat cgcgaaagat
     1381 aggacggtgg agtcgacttg aatgccttcc caaccgggaa cccacttggt ccaatcgggc
     1441 gatatgctgt tacgaaggat gcgcacactc gaaaaagagg ctgcaatcgc catggctccg
     1501 ccaagcatcg ccagcaggga catctccgag accaggaggc gcgccagccg tgtgcgacta
     1561 gcccccagcg ccgagcgcat ggcaagttcc ttctgccgcg taattatccg ggtaaacaaa
     1621 agattcgcaa aattggcgca agccagaagt agcacaaatg ccgcagcccc ctgaagcagc
     1681 aggaagaggg gcaaagtaaa aaggtaaagt tctttgcgaa gctgcaatat gttgagaacg
     1741 cggcctgcat tcgtgtttgg gtagagttcg gcgagccttt gggagagatt gcttagcgcc
     1801 gccgcagctt cccgaactgt gatgcctggc ttcagccggc caagagctgc gagagacaac
     1861 tgagcgcgat cggacttctc cgccggagta agcgccaaag gaacccacaa ttccatgggc
     1921 accgggaagt caaagtccgg gggcatgatt ccggcaatgg tgtagttgcg cgcgttgagg
     1981 cggatggtct tgcccaggat ttgcgggtca cccgtgaatc tggattgcca gaagctgttg
     2041 ctgatgatga ctgactgatc ggagccgggc tgttcttcct ccgcggaaaa gcgacgccct
     2101 tgcgttggcc cgaccccgag aaccgaaaag aagtttgcgg acacgcggca gccgcgcgcc
     2161 atgtccactc ggccctcgga agaaagattg aagtcgccgc actgatagct tgcgacgtcc
     2221 tcaaacaact gcaggttggc gcgaaggtcg gaagtgtcgc caggtgaaat ccatcgggcg
     2281 tcgaagcctt cgtcgattcc gcggttttcc caaatgagga ccagcgattc caaatcgtgg
     2341 aaacgatagg gatgcaacag cagggcgttc accatgctga aaatggcggt gttggttcca
     2401 ataccgaaag ccagcgtgag cacggcgatg gcagtgaagc cgggagactt gcgtagcatg
     2461 cgaagagcaa agcggaggtc ctgcaggaaa gtctcgagga aaggcaggcc tcgatgttcc
     2521 cggtagattt cttttgtttg ttcgagaccg ccgaagccga ggagcgcttc gcggcgagcc
     2581 tgttcggggc tcataccttg gcgaagattg tgctcgacgg ccatgtccaa atgtgagcgc
     2641 aggtcggcgt cgagctcgcg gtcgaggctg cgacggcgaa acagagcacg gaaacgcgtc
     2701 gccagaatgc gaatttggtc ggtcatgagg tggccttcag cacgcgatta atcacggcag
     2761 ccatctggtt ccaacccgca gcttcgtctc gaagctgctt gcggcctcgt cgtgtgagcg
//
LOCUS       DASTPM010000081         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_60593, whole genome shotgun sequence.
ACCESSION   DASTPM010000081 DASTPM010000000
VERSION     DASTPM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60593"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VFR42_01470"
     CDS             <1..747
                     /locus_tag="VFR42_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367846.1"
                     /translation="VTGLLEGKQVAFLARHGRGHRIPPTEINYRANIYALKLLGVDRI
                     ISVSAVGSLKEELAPGEFLVADQFVDRTRHRVSTFFGEGLVAHVTFDKPTCQEVSHVL
                     ADASVHCGVKVHRKGTYICMEGPQFSTLAEAHMHRGLGFDVIGMTNVTEAKLAREAEL
                     CYGAIAMITDYDCWHPDHESVTGAQIIATLNQNAENAQRVLREAVKSMPAAHQCKCGS
                     ALAHALITDPKLVPKSTKKRLAAIIGKYIS"
     gene            830..1738
                     /locus_tag="VFR42_01475"
     CDS             830..1738
                     /locus_tag="VFR42_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0367847.1"
                     /translation="MSLLVVGSVAFDAIESPYGKVDRIIGGAATYFAVAASFFTHVNL
                     VGIVGDDFSEKDQAIFRGRKIDTAGLERASGKCFFWAGKYSQHLNERETLVTELNVFA
                     DFNPKLPEKFRSSSHVFLANIAPGLQSSVLKQVKGKPKLAALDTMNYWIERTAADLRE
                     TLRHVDILMINDSETRQLSNEHNLLLAARHIFKLGPKTLIVKRGEYGAMMVDKRGVFC
                     VPAFPLEEPHDPTGAGDSFAGGFMGYLAGCGSSNDAALRRAMVYGSVLGSFAVEEFGP
                     ERLRKLKPKEIHARARHFLKLTQFKL"
     gene            1791..>2447
                     /locus_tag="VFR42_01480"
     CDS             1791..>2447
                     /locus_tag="VFR42_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU0367848.1"
                     /translation="MLPVSTVAYRLLWAALLGAAIGVERNIRKRPAGMRTGVCVCLGA
                     ALFTIISAEFAKLTGDPSTTRIASNIVQGIGFLGAGVILRERGNVIGVTTAAAIFAQA
                     AIGMGAGAGLYRVTGIAAVILLFALDGLIYIENLLSIKSRLTLFRISNGSAIDLMPEI
                     HRLFNEMNVKLENLRVSTSGDKHIIQFDSDVRHDQRHKALSALTRPGITVEMLPVERQ
                     Q"
BASE COUNT          506 a          840 c          602 g          499 t
ORIGIN      
        1 gtcaccggcc ttctcgaagg caaacaggta gctttcctcg cgcgccacgg tcgcggccat
       61 cgcattcccc ccaccgaaat taattaccgc gccaacattt acgccctcaa acttctcggc
      121 gtcgaccgca tcatctcggt cagcgccgtg ggctcgctaa aggaggaact tgcccctggt
      181 gaattcctcg tcgccgatca attcgtcgat cgtaccaggc atcgcgtctc cacttttttc
      241 ggcgaaggtc tcgtcgcgca tgtcaccttc gacaagccga cgtgccaaga ggtctcgcac
      301 gttctagccg acgccagcgt tcactgcggc gtcaaagtcc accgcaaggg cacttacatc
      361 tgcatggaag gcccgcaatt ttccacactt gccgaagccc acatgcaccg cggcctcggc
      421 ttcgacgtca tcggcatgac caacgtcacc gaggccaaac tcgctcgcga agcggaactc
      481 tgctacggcg ccatcgctat gatcacggac tacgactgct ggcatcccga tcacgaatct
      541 gtcaccggcg cgcaaatcat cgccacgctc aatcagaacg ccgaaaacgc tcaacgcgtt
      601 ctccgcgaag ccgtcaaatc catgccagcc gcgcaccagt gtaaatgcgg ctccgccctg
      661 gcccacgcgc tcatcaccga tcccaagctg gtgcccaaat ccaccaaaaa gcgcctggcc
      721 gcaatcatcg gcaaatacat ttcttgaggt tgtagcggct gccttctttg gcgggcgctt
      781 tgttttgctg cggcgcatct acgccgcttt ctcattacct ggagggtgaa tgtcacttct
      841 cgtcgtaggt tctgtagcat tcgacgccat tgaaagcccg tacggcaaag tcgaccgcat
      901 catcggcggc gccgcgacct atttcgccgt agccgccagc ttctttacac atgtgaatct
      961 tgtcggcatc gtgggcgacg acttttctga aaaggaccaa gccatcttcc gcgggcgcaa
     1021 aattgacacc gccggcctcg aacgcgcctc cggcaaatgt ttcttctggg ctggcaaata
     1081 ctcccagcat cttaacgagc gtgaaactct tgtaaccgaa ctcaacgtct tcgccgactt
     1141 caaccccaag cttccagaaa aatttcgctc ctcgtcgcat gtcttcctgg ccaacattgc
     1201 acctgggctg caaagcagcg ttctcaagca ggtcaaaggc aaacctaagc tagccgccct
     1261 cgataccatg aactactgga tcgagcgcac tgccgccgat ctacgcgaaa ccctgagaca
     1321 cgttgacatc ctaatgatca atgattcgga aacccgccag ctctccaacg agcacaatct
     1381 ccttctcgcc gcccgtcaca ttttcaagct cggccctaaa acgctcatcg tcaagcgcgg
     1441 cgaatacggc gccatgatgg tggacaagcg tggcgtcttc tgcgtcccgg catttccact
     1501 cgaagaaccg cacgatccca ccggcgcggg cgactcattc gccggaggtt tcatgggcta
     1561 tctcgccggc tgcggcagtt cgaacgatgc cgccctccgc cgggccatgg tctacggctc
     1621 cgtcctcggc agctttgccg tcgaagaatt cggccccgaa cgcctccgca aactcaagcc
     1681 gaaggagata cacgccagag cacgccactt cctcaaactc acgcagttca agctgtagcg
     1741 cgtgtagaat ccatttcgct gccggccggg ccaagcgctg gcgcaacaaa atgcttcctg
     1801 tctccaccgt cgcataccgc cttctctggg ctgccctcct gggcgccgcc atcggcgtcg
     1861 agcgcaacat ccgcaagcgc cccgccggaa tgcgcaccgg agtctgcgtt tgtctcggcg
     1921 cagctctttt caccatcatc tcagcggagt tcgcgaagct aacgggcgat cccagcacca
     1981 cgcgcattgc ttccaacatt gtccagggca tcggctttct cggtgccggc gtcatcctgc
     2041 gcgagcgcgg caacgtcatc ggagtaacaa ccgccgccgc aatcttcgcc caggccgcca
     2101 ttggcatggg cgccggagcc ggtctttatc gcgttaccgg aatcgctgcc gtgattcttc
     2161 tgttcgctct cgacggtctg atctatattg aaaatcttct tagcatcaag agccgcctca
     2221 cgctgttccg tatcagcaac ggttccgcaa tcgacttgat gccggaaatc caccgcctct
     2281 tcaacgagat gaacgtcaaa ctggagaacc ttcgcgtctc gacgtcgggc gacaagcaca
     2341 taattcagtt cgactccgac gttcgccacg atcagcgcca caaggctctc tccgccctaa
     2401 cgcgccccgg cattaccgtc gaaatgctgc ctgtcgaaag gcagcag
//
LOCUS       DASTPM010000082         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_175839, whole genome shotgun sequence.
ACCESSION   DASTPM010000082 DASTPM010000000
VERSION     DASTPM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175839"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1122)
                     /locus_tag="VFR42_01485"
     CDS             complement(292..1122)
                     /locus_tag="VFR42_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0367849.1"
                     /translation="MKCFRMALPASFLLVSLTLPTIGQTSDDTRKESFVPMQAQSQVS
                     TAAATPAERQLSGEEMGDLYMARKQYHEAMEQYQLLSQQNPHNAVYLNKLGIALHQQA
                     ALGAALKYYERAVKTDPKYADAENNIGTIWYQRKKYGKAVRAYQKAIKMNNDMPVLYS
                     NLGYAYFSEGRYEDSIAAFRIALAKDPQFFERGSSRTGSILQDRSVPDRGRFYFLLAK
                     SFAEAGNIERSVFYLRKAKEENYDKFNDVKKDPAFAAVLKDPVVIELLAPKTADSTQP
                     "
     gene            complement(1147..>3512)
                     /locus_tag="VFR42_01490"
     CDS             complement(1147..>3512)
                     /locus_tag="VFR42_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU0367850.1"
                     /translation="RTGYRPESDENALGQYTVQKSTLDIRPSKLSYFVGGNALDVQFT
                     GKTIRAIRPLGGGNAIDTAEIEPELITNLFDSAREKRRPVRYDDLPPVLVHAILSAED
                     KRFFDHGGFDFVRIAGAAWADLRHTSSHFQGASTITMQVARTFFFTNERTMRRKVAEA
                     MVSLELEQRYTKQQIFELYANEVYLGNRGSFGIRGFSQAAVAYFGKDLRQLSLAECAY
                     LAGIIRAPNYYSSADRHPERGLQARDRVLTQMLENKYINDADLEEAKKTQLKIVHASV
                     AGSEAPYFVDMVKDHLLDKYSENELLSENFRVYTTLDPALQRAAAAALEAGMKNVDVL
                     LAKKYERWQKELEKKGSAEAVPQAQGAMVALDPRTGEIKAVIGGRDYGQSQLNHALAH
                     RQPGSVFKPFVYAAAFDNAIDGVQPVVTPATTIDDEPTTFEYDGVDYTPNNYGQRFLG
                     RVTARDALTNSLNVATVKVAELIGYGRVVQIARQMGLGNNIRATPAVALGAYEMTPVD
                     VAAGYTTFANLGTRTEPQFIRNVIGADGTVLEKVTPQTHLALDPRVAYLVTSILKDVL
                     NHGTGAGVRARGFTLPAAGKTGTSRDGWFAGFTSNLLCVVWVGFDDNRDIGFTGGAVA
                     APIWADFMNHATALSTYRDVKDFDPPEGVQSVLIDPESLELATSNCPTTRQEVYIAGS
                     APTQYCEIHGSHNVFTSTGSWLSHVFGGGQPKEPPKDADGKPIPYDPNRPPNATGQGQ
                     PPEPVEAKDKEDEKKKNPLKKIFGIFGGKKKDSDKPEPADPPKPDKP"
BASE COUNT          686 a         1087 c          987 g          752 t
ORIGIN      
        1 gcccagccag tccaggcgcg gagaggcgcc ggcgttttca aagggaaaca caagatacac
       61 gccagcttga gagacggcag aggcgcgcgc aggcgcttgc tgggcattga gcgaggccac
      121 ccagaacaat gcacaacaaa aaagaaaagc acgggcaatg aaacgatgcg gggacatggt
      181 tcctaggaat tgtacagcga cttgaggctg aagggattac cacccgcgga tacgtacgtc
      241 gaatggtaaa cgccgtcaac aggtccgcta cgcaaaaccc ggtgttacca attacggttg
      301 cgtggagtcc gccgtcttcg gcgccagcag ctcgatgacc accggatcct tcaacaccgc
      361 cgcaaacgcc ggatcctttt tcacgtcatt gaacttatcg taattttctt ccttggcctt
      421 gcgcaaatag aacacgctgc gctcgatatt cccggcttcc gcaaacgatt tcgccagcag
      481 gaagtagaat cgcccgcggt ccggcaccga gcggtcttgc aaaatggaac cggtacgcga
      541 ggagccgcgc tcaaaaaatt gcgggtcttt ggccaaagcg atgcggaagg cggcgatgga
      601 atcttcatac cggccttcgc tgaagtacgc atagcccaga ttgctgtaca gcaccggcat
      661 gtcgttgttc atcttgatgg ctttctgata ggcgcgcacc gctttgccgt atttcttgcg
      721 ctgataccag atggtgccaa tgttattttc ggcatcggca tacttgggat cggttttcac
      781 cgcgcgctcg taatatttca gcgccgcgcc cagagcggcc tgctggtgca gcgcaatccc
      841 cagcttattc aagtacaccg cgttgtgcgg attctgctgg ctcagcagct ggtattgctc
      901 catggcttcg tggtactgct tgcgcgccat gtacaggtcg cccatctctt cgccgctgag
      961 ctggcgctcg gccggtgtgg ccgccgcagt ggacacttgc gactgcgcct gcatcggaac
     1021 gaatgattct ttgcgggtat cgtcgctggt ctggccaatg gtaggaaggg tcaggctaac
     1081 gaggagaaaa ctagcgggca acgccattcg aaaacatttc atggcgagtc tcctttgttt
     1141 ctccggctaa ggcttgtccg gtttcggtgg atcggccggt tcgggcttgt cggagtcctt
     1201 cttcttacct ccaaagatac caaaaatctt cttcaaggga ttctttttct tttcgtcttc
     1261 cttgtccttg gcttccaccg gctcgggcgg ctgcccttgc ccggtagcat ttggcggacg
     1321 attgggatcg tacggaatcg gctttccatc ggcgtccttc ggcggctcct tcggctgccc
     1381 tcccccgaaa acgtgcgata gccaggaacc cgtggaggtg aaaacgttgt gactcccatg
     1441 aatttcgcag tactgcgtcg gcgccgagcc ggctatatag acttcctgcc gggtcgtcgg
     1501 gcaattggag gtggccagct ccagtgattc cggatcgatc agcacggact gcacgccttc
     1561 cggcggatcg aaatccttta catcgcgata ggtagaaagc gctgtggcgt gattcatgaa
     1621 atcggcccag atgggcgcag ccacggcgcc tccggtaaac ccgatgtcgc gattatcgtc
     1681 aaatcccacc cacaccacgc acaacagatt cgacgtgaat cccgcaaacc agccatcgcg
     1741 cgacgtcccc gtttttcccg ccgccggcag cgtaaagccc cgcgcgcgaa ccccagcgcc
     1801 ggtgccatga ttcaggacat ctttcaggat gctggtgacc aggtacgcca cccgcggatc
     1861 aagcgccagg tgcgtttgcg gcgtgacctt ctccagcacc gtcccatccg ccccgatcac
     1921 gttgcgaata aattgcggct cggtgcgcgt gcccagattc gcaaacgtgg tgtatccggc
     1981 ggccacatcc accggcgtca tttcataagc gcccagcgca acggccggcg tggcgcgaat
     2041 attgttgccc agtcccatct ggcgcgcgat ttgcaccacc cggccatagc caatcagttc
     2101 ggcgactttt accgtggcca cgttcagcga attggtcagc gcgtcgcgcg cagtaacccg
     2161 gcccaaaaat ctctgcccgt aattgttcgg cgtgtaatcc actccgtcgt attcaaacgt
     2221 agtcggctcg tcgtcgatgg tcgtcgcggg cgtgaccacc ggctgcacgc catcaatggc
     2281 gttatcaaac gccgctgcgt aaacgaaagg cttgaacacc gaacccggct gccggtgcgc
     2341 cagcgcgtga ttcagctggc tctgcccgta gtcgcgtccg ccgatcaccg ccttgatttc
     2401 cccggtgcgg ggatcgagcg ccaccatcgc gccctgcgct tgcggcaccg cctccgcact
     2461 tcccttcttc tccagctcct tctgccagcg ctcgtatttt ttcgccagca gcacgtccac
     2521 attcttcatg cccgcttcca gcgccgcggc cgccgcgcgc tgcagcgcag gatcgagcgt
     2581 ggtgtatacc cggaaatttt cgctcagcaa ttcgttttcc gaatatttat ccagcaaatg
     2641 gtccttgacc atgtccacga agtacggagc ttcgctgccg gccaccgacg cgtgcacgat
     2701 cttcaactgc gttttcttgg cttcctccag gtccgcatcg ttgatgtact tgttttctag
     2761 catttgcgtc agcacccggt cgcgcgcctg caacccgcgc tccggatgcc ggtccgccga
     2821 agaatagtag ttgggcgcgc gaatgatgcc ggccagataa gcgcattcgg ccagcgagag
     2881 ctgccgcaaa tccttgccga agtaagccac cgcggcttgc gaaaatccgc gaatgccaaa
     2941 actgccgcga ttgcccagat aaacttcatt cgcatacaac tcgaaaattt gctgcttggt
     3001 gtagcgctgc tcgagctcca gcgaaaccat ggcctccgcc actttgcggc gcatggtgcg
     3061 ctcgttggtg aaaaagaaag tccgcgccac ctgcatggtg atcgtgctgg caccctggaa
     3121 gtggctgctg gtgtgccgca aatcggccca cgcggcgccg gcgatgcgca caaaatcaaa
     3181 gccgccgtgg tcgaaaaagc gcttgtcttc cgcggaaaga atggcatgca ccagcaccgg
     3241 cggcagatcg tcgtagcgca ccgggcggcg cttctcgcgc gcgctatcaa acaaattggt
     3301 gatcagctcc ggctcgattt ccgcggtatc aatggcattg ccgccgccga gcgggcgaat
     3361 cgcgcgaatg gtcttgcccg taaattgcac gtccagcgcg ttgccgccaa caaaatagga
     3421 gagcttcgac gggcggatat ccaaggtgct tttctgcacc gtgtattggc ccaaagcgtt
     3481 ttcgtcggat tccgggcgat agccggtgcg ct
//
LOCUS       DASTPM010000083         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_521980, whole genome shotgun sequence.
ACCESSION   DASTPM010000083 DASTPM010000000
VERSION     DASTPM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521980"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1447
                     /locus_tag="VFR42_01495"
     CDS             200..1447
                     /locus_tag="VFR42_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0367851.1"
                     /translation="MKKIRCYYPPNYQPNGPQAFPQSNMVVLVDTEAGITGIGQGGSP
                     DTVRNMARSVIGKNAFETEFIWQHGFMDGFYSPGKERLHALGAIDLALWDIKGKALET
                     PLYELFGGKARQHIELYATNGVPQGAVPQADLQRMSLKERAAATMAAGYRVFRVDSAI
                     RPTPAGGPPAGGRGGAGRGAVSPDAPVGVGGRGTFNSRSRIREIAEAAAQIREGVGPD
                     GDWMIDLHQSFDFAESVEVCNRIASARPFCAEDPLREEQFRTQLPKLRLMTPIPLAAG
                     EEWGTRADFAPLVEQRDIDFARASLPNVGGITEMLKIMALCDLHKVGIMPHFTGPIAT
                     VGHMQTMMAFPGQVLMEYNQGERPVPYLPEFLECRNGKVWPNDRPGLGVSVDEKQLTP
                     IETVTEAGPGPTYLRPDGSPTHW"
     gene            complement(1467..1652)
                     /locus_tag="VFR42_01500"
     CDS             complement(1467..1652)
                     /locus_tag="VFR42_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367852.1"
                     /translation="MTAATFNNGAINSNLTVNGAPGLNIVNLNNFTLSGNLTLNGAPG
                     TQVVININGNFNPCARI"
     gene            complement(1995..>2199)
                     /locus_tag="VFR42_01505"
     CDS             complement(1995..>2199)
                     /locus_tag="VFR42_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367853.1"
                     /translation="MTRQLMEANFRQREEPLAEVLGLLSTLLENWQGVQQLVAGERAP
                     QPLVSSYAVAQGTAAYTPQAWSL"
BASE COUNT          462 a          643 c          681 g          413 t
ORIGIN      
        1 cgtgccatgc tgcttcacgg tttatgatgg aatgcgcgca gacctctaaa ttgcgtgttg
       61 gcgccaaata gcaagtgaat atgaaatgag gacgaatatg aaattggatc gcagggtgtt
      121 caccaaagga ctggcagctg cgctggcgct gccctcctgg acaacaaagg catccgcagc
      181 aaagctaccg gtcaatcgcg tgaaaaagat ccgctgctat tatccgccga actaccagcc
      241 caacggtccg caggcttttc cgcagagcaa catggtggtg ctggtcgata cggaagccgg
      301 catcaccggc atcggtcagg gcggatcgcc cgataccgtg cggaatatgg cgcgcagcgt
      361 gatcggcaag aacgcttttg aaaccgagtt catttggcaa cacggattca tggatgggtt
      421 ctactcacca ggcaaagaac gattgcatgc cctcggcgct attgatttag cgttgtggga
      481 catcaagggc aaggcgctcg aaacccctct ctacgagttg ttcggcggca aggcgcgtca
      541 gcacatcgaa ctatacgcga ccaacggcgt gccgcagggc gcggtcccgc aagccgatct
      601 gcagcgaatg agtctgaagg agcgcgccgc cgcgaccatg gcggctgggt accgggtgtt
      661 ccgggtggac agcgcgatta ggcccacacc cgccggtgga cctcccgcgg gaggtagggg
      721 cggcgccgga cgcggggcag tttcgccgga tgcgcctgtc ggtgtgggag gccgcggcac
      781 cttcaactcg cgctcacgca tccgcgaaat cgccgaagcg gcggctcaga ttcgcgaagg
      841 ggtcgggccg gatggcgact ggatgatcga ccttcaccag agcttcgatt ttgccgaatc
      901 ggtcgaggtc tgtaatcgga tcgcgtcggc acggccgttt tgcgcggaag atcctctgcg
      961 ggaggaacag ttccgcacgc agctccccaa gctgcggctt atgactccga ttcctctggc
     1021 cgcgggagaa gaatggggga cccgcgcgga tttcgctccg ctcgtcgaac agcgcgatat
     1081 cgactttgca cgggcctccc tgccgaacgt cggcgggatt accgagatgc tcaaaatcat
     1141 ggcgctctgc gacctgcaca aagtcgggat tatgccgcac ttcacaggcc cgatcgcgac
     1201 ggtcggccac atgcagacaa tgatggcgtt cccggggcaa gtgctgatgg aatacaacca
     1261 gggcgaacgg cctgtgccgt atctgcccga gttcctggag tgccgaaacg gcaaggtgtg
     1321 gcctaacgac agacccggac tcggcgtctc cgtcgatgaa aagcaactca cgcctatcga
     1381 aacagtcacg gaagcaggcc ccggcccgac ctatctgcgt ccggacggat cgcccacgca
     1441 ctggtaagaa gcactggcca agcgaactat attcgggcgc acgggttgaa attgccgttg
     1501 atgttgatga cgacctgcgt gcctggcgct ccgttcaacg tcagattacc gctcagtgtg
     1561 aagttgttaa ggttgacgat gttgagtccc ggggcgccgt tgaccgtcag gttcgaattg
     1621 atggcgccgt tgttgaaggt cgcagccgtc attgtaggcg ttagcgccgc cagttgagtg
     1681 taagtcctca gaatcagcaa tgccctttag gttgccggga aacacgcggc atcaccgaac
     1741 ggcccggccc cgctccaagt ggtcggctac ccggagaaac caccaaagac cgttgttagc
     1801 gatcatcggt ccactgcgcc cggttcgaag ccgtccgctg gtttgtcacg cgacgacaaa
     1861 gcccgaccgc caggatcctt aacggaacgg tattgagggc aaacggtagt ccttcccact
     1921 ccgcttggaa tcgagaagcg cgcgaacggc aagaatgtgg atccgcctcg cgatcacaat
     1981 ccgaagcctg ggccttataa gctccaggct tgcggggtgt atgctgcggt tccttgagca
     2041 accgcataag aggaaacgag aggttgcggc gcccgctcgc cggcaacgag ctgctgaacg
     2101 ccttgccaat tctcgagcag cgtgcttaac agcccaagga cttccgccag tggttcttcg
     2161 cgctgccgga aattcgcctc catgagttga cgcgtcata
//
LOCUS       DASTPM010000084         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1012034, whole genome shotgun sequence.
ACCESSION   DASTPM010000084 DASTPM010000000
VERSION     DASTPM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012034"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VFR42_01510"
     CDS             complement(<1..640)
                     /locus_tag="VFR42_01510"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0367854.1"
                     /translation="MNQRRLWQILPGILTMAWWCPSAFSQAAAPSPAVAKSSASEMTL
                     YRNGRIYTNDATAPWAEALLVRGEEILAVGDDDEVSALTERGAKVVDLEKHFVMPGFN
                     DAHVHIGGAGADWLAVRLFGAATVAEMQKRLAAAVAGHKQGEWITGFGWDHTLWPEKK
                     FPNRQQLDEVSPKNPVILTHVSGHVAVANSMALQLAGITKDSPNPPGGEIEKD"
     gene            complement(637..1941)
                     /locus_tag="VFR42_01515"
     CDS             complement(637..1941)
                     /locus_tag="VFR42_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0367855.1"
                     /translation="MKLNGDCCLKSLVLSGILLAMPIRPVISFPANRAAHFTQLQTNP
                     QVRSALDWFAAHHSWIDDEQARLTEIPAPAFQESERAAAVKVLLSAVGLEVTTDKTGN
                     VIGLLRGTLDKEVVVLSAHLDTVFPAGTEVKVHREKSRMTAPGISDNGAGLAALVAVA
                     RAIHEARIQPQRGILFVANVGEEGEGNLRGMRAVVDAYRDRLKAVIVLDGSSVDHVTT
                     KALASRRVEVVITGPGGHSWSDFGVPNPINALVRGSVRFISTRIPSTPRTTFNLGQIE
                     GGTSVNSIPHEARLKVDLRSEKEDELTRLESALRECVAAGVRDELETSRDRSKGKLEW
                     KLDSLGERPGGELAANSALLAALRAADEFVGNQSRVERASTDANIPLSLGIEAIAIGA
                     GGSGGGAHSLQEWYESDGREVGLKRVLLTLLATAGVAAEKTR"
     gene            complement(1938..>2741)
                     /locus_tag="VFR42_01520"
     CDS             complement(1938..>2741)
                     /locus_tag="VFR42_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367856.1"
                     /translation="TTESAVKFAISHNLPSMYVTEDTIRTDPETVKRLYSTAIRSGAR
                     AIVLCDTVGHATPPGAYNLVKFTIEEVVKPSGEKIRVDWHGHCDRGLAVANSLWAIAA
                     GAHQVHAAANSLGERVGNTPMELMLVNLRLMGLIEQDLSHLKEYCETVATATHTVIPP
                     NYPVIGKDAFRTATGVHAAAIVKAFHKGNSELANIVYSGVPSHLFGLEQIIEIGPMSG
                     KSNVLFWLERRGIPADDALVARLLDAAKKSARVLTEGELLALSAGVTPH"
BASE COUNT          522 a          923 c          729 g          567 t
ORIGIN      
        1 cgtctttctc gatttcaccg cccggaggat ttggcgagtc cttcgtgatt cccgcaagtt
       61 gtaatgccat cgagttcgca acggctacgt gcccggaaac atgcgtcagg atcactggat
      121 tcttgggcga cacttcgtcg agttgctggc ggttcggaaa tttcttttcc ggccatagcg
      181 tgtggtccca tccgaatccg gtaatccatt ctccctgctt atgcccggcc actgcggccg
      241 ccagccgctt ttgcatttca gcaaccgtcg ccgctccgaa caaacgcacc gccagccaat
      301 ccgcccctgc gcctcctatg tgcacgtggg cgtcgttgaa gccgggcatc acaaaatgtt
      361 tttccagatc gacaaccttc gcgcctcgtt ccgtgagcgc gctcacctcg tcatcatcgc
      421 ctaccgcaag aatctcctcg ccacgcacca acaaagcctc cgcccacggc gcggtggcat
      481 cgttggtgta gattcttccg ttccggtaca gcgtcatttc tgacgcggat gacttcgcta
      541 ccgccggcga cggcgccgca gcctgcgaga atgccgaagg acaccaccac gccatcgtca
      601 gaatccctgg gaggatctgc cacagccgac gttgattcat cgagttttct ccgccgccac
      661 tcccgcagtt gccagcaagg tcagcaacac acgctttaaa ccaacttcac ggccatccga
      721 ctcgtaccac tcttgcaggg agtgcgcgcc gccgccgctg ccgcccgccc caatcgcaat
      781 cgcttcaata cccagcgaca acggaatatt cgcatcggtg gatgcgcgtt ccacgcgcga
      841 ctggttcccg acaaactcat ccgccgcgcg caacgccgcc agcagcgcgg aattcgccgc
      901 aagttcccca cccggccgct cgcccaggga atccagtttc cattccagct tccctttcga
      961 acggtcgcgc gaagtttcca gctcgtcgcg aacgcccgcg gcaacacatt cgcgcagcgc
     1021 gctttccaga cgcgtcaatt cgtcttcttt ttccgaacgc aggtccactt tcaaccgcgc
     1081 ttcatgcgga atggaattca ccgaggttcc gccttcaatt tgccccagat tgaaggttgt
     1141 ccgcggcgtc gaaggaatac gcgtgctgat aaagcggacg gaaccccgta ccagtgcgtt
     1201 gatgggattg ggaacgccga agtccgacca gctatgcccg ccggggccgg tgatcacaac
     1261 ttccacgcgg cgcgacgcca gcgctttcgt ggtgacgtgg tccacgctcg aaccatcgag
     1321 cacgatcacc gccttcagtc ggtctcggta cgcgtccacc acggcgcgca tcccgcgcaa
     1381 attcccttct ccttcttcgc caacgttggc cacaaacaaa atgccgcgtt gtggctgaat
     1441 cctggcctca tggatcgcgc gagccacggc taccagcgcc gccagtccgg ctccgttgtc
     1501 ggagattccc ggcgccgtca tgcggctctt ttcgcgatga actttcactt cggtgcctgc
     1561 cggaaaaacc gtgtcgaggt gcgccgacaa caccacgact tccttgtcca aggtgccgcg
     1621 cagcaggccg atcacatttc ccgtcttgtc ggtggtaact tccaatccaa ctgccgacaa
     1681 gagtaccttc acagccgcgg cgcgctcgga ttcctgaaac gccggcgcag gaatctccgt
     1741 cagccgcgct tgttcgtcgt caatccagga atgatgcgcc gcaaaccagt ccagcgcgga
     1801 acgcacttgc ggatttgtct gcagttgcgt gaagtgcgcc gcgcggttgg ccggaaagga
     1861 gatgaccggg cgaatcggca tggccagcag aatacccgaa agcaccaggc ttttcaggca
     1921 gcaatcgcca ttgagtttca atgcggagtc actccggcgg atagcgcgag cagctcgcct
     1981 tcggtcagca cgcgcgccga tttcttggcg gcatccagga gtcgtgccac caacgcgtca
     2041 tccgcgggaa tgccacgccg ttccagccag aacagcacgt tcgacttgcc gctcatcggc
     2101 ccaatctcga ttatttgttc cagtccgaac aaatgagaag gcacgccgct gtaaacgatg
     2161 ttggccagct cggaattccc cttatgaaat gctttgacga tcgcggccgc atgcacgccc
     2221 gtcgccgtcc gaaacgcatc ttttccaatc accggatagt tcggcggaat cacggtgtgg
     2281 gtggccgtcg ccaccgtttc gcaatactcc ttcaagtgcg acaagtcctg ttcgatcaag
     2341 cccatcaggc gcaaattgac cagcatgagt tccatcgggg tgttgcctac gcgttcgcct
     2401 agcgaattcg ccgccgcgtg cacctgatgc gcgccggcgg caatcgccca cagtgaattc
     2461 gccaccgcca gtccccggtc acagtggccg tgccagtcca cacgaatctt ttccccggat
     2521 ggcttcacta cttcttcgat cgtgaatttc accaggttgt atgcgccggg cggcgttgcg
     2581 tggccaaccg tgtcgcacag cacaatcgcg cgagcgccgc tgcgaattgc cgtcgaatac
     2641 aagcgcttca ccgtctccgg atcagtgcga atcgtgtctt cggtaacgta catggacggc
     2701 aagttgtggc ttatcgcgaa tttcaccgcg ctttcggtgg t
//
LOCUS       DASTPM010000085         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1213694, whole genome shotgun sequence.
ACCESSION   DASTPM010000085 DASTPM010000000
VERSION     DASTPM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213694"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFR42_01525"
     CDS             <1..529
                     /locus_tag="VFR42_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367857.1"
                     /translation="RIEPAVVNARSPGVRDLVDGGRFDARRRALRKIQSPETRRGREE
                     LNTHTLAAVAQITEKHDAALDLFLSFGVGDRQELAIIHFVFQHKKPAVRADHQGLARL
                     AEFFAIVSASLRLHLHFAKDASAAPGSGKLDRGHHAFIIEKALEGVNWPAGQVYRIKK
                     RVAGRLVLCRERVLQ"
     gene            634..1998
                     /locus_tag="VFR42_01530"
     CDS             634..1998
                     /locus_tag="VFR42_01530"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU0367858.1"
                     /translation="MTLDEFIDGRRDQNLTELNEFLRIPSVSAKSEHKPDVERAAKWV
                     ADHLRSAGFKTIEIVPTAMHPLVYAESLEVPGKPTVLFYGHYDVQPAEPLHLWTSPAF
                     EPTVRDGNIYARGTADDKGQVHIHLKALQGLNEMNGKFPINIKVLIEGEEEVGSVSLW
                     NFVQKNKEKLAADALVVSDTSMLGKGVPSITYGLRGLNYYQVELTGPERDLHSGVYGG
                     AVPNPLTILTELFAKLHDKNFRVTVPGFYDGVAKLSAKERKALNALPWNRKAFQQAVG
                     AAGYCGEKGFTTVEQIWTRPTLELNGIWGGYQGEGAKTVIPSKAYAKFSTRLVPGQDP
                     QRIAKLVEKHVRKLLPKTVKCKFDVLSTGKPWAASYSAPIFQKAQAALEKGFKKKAVF
                     IREGGSIPFVTQMHDTFKVPCVLIGFGLPDENAHAPDEHLNLENYFSGIKAIAHFYQD
                     LATL"
     gene            complement(2029..2508)
                     /locus_tag="VFR42_01535"
     CDS             complement(2029..2508)
                     /locus_tag="VFR42_01535"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEU0367859.1"
                     /translation="MQELKIVPISTAVAETVRATMKAPVYGFPAHREVAAGRAPCRQC
                     LQLIRPGAEELILFTYDAFHGQSVPPSPGPVYIHAAPCALFAGNGHFPQQYHGQPLTL
                     EVFGENRARLTERLVNGTDGDAELQEIFQDTNVKFVHVRSTTAGCYLFRVERAAQAG"
     gene            2597..2974
                     /locus_tag="VFR42_01540"
     CDS             2597..2974
                     /locus_tag="VFR42_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0367860.1"
                     /translation="MVPRLPARQGHFPSGRTVTEVAAGKQALDALEQGNFDAIVTDAR
                     VPRLAGIELVRPLHKFGSYTPVIIFVSGFVGLTLPDAYDLGVEAVLSKPCPGKELVNT
                     LRRSIERRNLVFDSDEDVPATGR"
BASE COUNT          651 a          825 c          915 g          612 t
ORIGIN      
        1 cagaattgag cccgctgttg tgaacgccag aagcccaggc gtgagggatt tagtggatgg
       61 cggacgattc gacgccagaa ggcgcgcgct cagaaaaatc cagtccccag aaacgcggcg
      121 agggcgtgag gaactcaata cccacacgct ggccgccgtc gcacagatca ccgagaaaca
      181 cgatgcggca ctcgatctct tcctgagttt cggggttggt gatcgtcaag agctcgccat
      241 tatccacttc gtgtttcaac ataagaagcc cgccgtgcgc gctgaccacc agggtctggc
      301 acgtctcgcg gaattttttg ccattgtgag cgcgtccctg cgcctgcatc ttcattttgc
      361 gaaagatgcg agcgctgcgc cggggagcgg aaaactcgac cgtggtcatc atgctttcat
      421 catcgaaaag gccttagaag gggtcaattg gcctgcgggg caggtgtatc gcatcaagaa
      481 acgggtggcc ggtcggcttg ttctttgccg cgagcgggtc ttacaatgaa cgtcgcgcgg
      541 tgcgcagctc cacccagcgt ttgtgcctgc cgcaacattc ttaagtcctc attcgtagat
      601 atccttggaa aggaccagct ctcttacgtc cccatgacac tggacgaatt cattgatggt
      661 cgccgcgacc agaacttgac cgagttgaac gaatttctcc gcattccgag cgtcagcgca
      721 aaatccgagc acaagcccga tgtagaacgc gccgcaaaat gggtggcgga tcatctgcga
      781 tcggccggct tcaaaacgat cgaaattgtg ccaaccgcga tgcatccctt ggtgtacgcg
      841 gaatcactcg aagtgccggg aaaaccgacc gtactttttt acggccatta cgacgtgcag
      901 cccgccgagc cgttgcactt gtggacctcg cccgccttcg agccgaccgt gcgggatgga
      961 aacatctacg cgcgcgggac cgccgacgac aaagggcagg tgcacattca cctgaaggcg
     1021 ctgcaggggc tcaatgaaat gaacggcaag ttccccatca acattaaggt tttgatcgag
     1081 ggcgaagagg aagtgggcag cgtcagcttg tggaactttg tccagaaaaa caaagaaaag
     1141 ctggcggcgg atgcgctggt ggtttccgat acctcgatgc ttggcaaggg cgttccgtcg
     1201 attacctacg gcttgcgcgg gttgaattac taccaggtgg aattgaccgg gccggaacgt
     1261 gatttgcact cgggcgtata cggtggagcg gtcccgaatc cgctgacgat tctgacggaa
     1321 ctgttcgcga agctacatga caagaatttc cgggtgaccg tgccgggatt ttacgatggc
     1381 gtggccaagc tgtccgcaaa ggagcgcaaa gcgctgaatg cgctgccgtg gaaccgcaag
     1441 gcgtttcagc aagccgttgg agcggcgggc tattgtggcg aaaagggatt tacgacggtc
     1501 gaacagatct ggacgcggcc cacactcgag ttgaacggga tttggggcgg ctatcagggc
     1561 gaaggcgcga agaccgtgat tccttcgaag gcctacgcaa agttttccac gcggctggtg
     1621 cccggccagg acccgcagcg catcgccaag ctggtggaaa agcacgtgcg caagctgttg
     1681 ccgaaaacgg tgaagtgcaa attcgatgtg ctgagcacgg gcaagccgtg ggcggcgtcc
     1741 tacagcgcgc cgatatttca gaaagcgcaa gcggcactgg aaaaaggttt caagaaaaag
     1801 gctgtcttta tccgtgaggg cggatcgatc ccgtttgtca cgcagatgca cgatacgttc
     1861 aaggtgccgt gcgtgctgat cggcttcggg ctgcccgatg aaaacgcgca cgcgcccgac
     1921 gagcatctga acctggaaaa ttatttcagc ggcatcaagg cgatcgcgca cttctatcag
     1981 gatcttgcga cactgtaagc ggtgcgatcg ttttttttga cgaggggctc agcccgcctg
     2041 agcggcgcgc tccacgcgaa acaggtagca accggccgtt gtactccgga catgcacgaa
     2101 cttcacgttt gtatcctgga agatctcctg cagttccgca tcgccatctg ttccgttgac
     2161 tagtctttcc gtcagtcgcg cgcgattctc cccaaacact tcgagggtca gcggctggcc
     2221 gtggtactgc tgcgggaaat gaccgttacc tgcaaacagc gcgcaaggcg cggcatgtat
     2281 gtaaaccgga ccagggctcg gcggcacact ttgcccatgg aatgcgtcgt aggtaaacag
     2341 aatcagctcc tccgcgcctg gccgaatgag ttggaggcat tgccggcaag gtgcgcggcc
     2401 agcagcgact tcgcggtgag cgggaaagcc gtacaccgga gctttcatgg tggcacgcac
     2461 ggtttccgca actgcggtgg aaatcggaac gatcttgagt tcttgcatgg tgacctcgtt
     2521 ttggtgttcc tgacgaggcc aagtttggac gaatcgcggg tgtgcaatct atccggtatg
     2581 tgcgcgcaaa ttcgcgatgg ttcctaggtt accggcccgc cagggtcact tcccgtcggg
     2641 ccgcacggta accgaagtgg ccgccggaaa acaggcattg gatgcgctcg agcaggggaa
     2701 ttttgacgcg atcgtaaccg acgcgcgcgt gccgcgcctc gccggcatcg aattggtgcg
     2761 cccgctgcac aagttcggca gctatactcc ggtaattatt ttcgtcagcg gctttgtcgg
     2821 tctaacgctt ccagacgctt atgacctggg cgtggaagcg gtcttgtcga agccgtgccc
     2881 tggcaaggaa ctggtaaaca ctctgcgccg gagcatcgaa cggcgcaatc tggtttttga
     2941 ttcggacgag gacgttcctg ctaccggcag atgatcagat ccagcaaaga cttcaaaact
     3001 gca
//
LOCUS       DASTPM010000086         4690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_291322, whole genome shotgun sequence.
ACCESSION   DASTPM010000086 DASTPM010000000
VERSION     DASTPM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291322"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..650
                     /locus_tag="VFR42_01545"
     CDS             39..650
                     /locus_tag="VFR42_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU0367861.1"
                     /translation="MSNVRLPLFPLNVVLLPGSDLPLHIFEPRYRQMVRECLDTQTPF
                     GILLALPNGIAGTGCTAEILEVTKRYPDGRCDILTVGRSPFRVVHLFNDEPLLRGEVD
                     FLEDRPSPINSRICRELNDLYETCHTLIRGDYPRDLCSTQDSDISYEVAAKLPMELLW
                     KQCILELRSEAERQERLVAYLREWAPHLEKTTTLRARAANGLN"
     gene            737..2248
                     /locus_tag="VFR42_01550"
     CDS             737..2248
                     /locus_tag="VFR42_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367862.1"
                     /translation="MTIATSTHSSGGRARPATLGELRRIPDFDARAASRCVKDEIRTN
                     LLRAIERGETLFPGVQGYEDTVIPQIVNALLSRHNFILLGLRGQAKSRILRGLINLLD
                     TEIPVIAGCEINDDPLKPICRACRERIAAEGDNTPIAWMPRDLRYVEKLATPDVTIAD
                     IIGDVDPIRAARGGRDLSDELTIHYGLLPRANRGIFAVNELPDLAGKIQVGLFNIMQE
                     GDIQIKGYPLRLPLDVLLVFSANPEDYTARGKIITPLKDRIGSEIRTHYPATLEEGVA
                     ITTQEAWTGRDSAYRVEVPDYLRELVEEIAFQARDDKRVDKRSGVSQRLPISVVESVV
                     SSAEQRAARTGEKLTVARVADVYAAMPSITGKLELEYEGELKGADTVARELIRTASGR
                     IFSKRFSDVNFQPIIQWFELGGELKLAELASTAERYKQLANIKNLMDHVDRLGADKKD
                     QAHAAAAAEMILEGLWAHRRIGRSEERGFFAEKPRQPEPRESGPPRPPRRSYN"
     gene            2478..3755
                     /locus_tag="VFR42_01555"
     CDS             2478..3755
                     /locus_tag="VFR42_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0367863.1"
                     /translation="MKFTKFTKYTGEPADSIDLEELIKRLGDFFLQSGFESQYYGITE
                     FDPQRSLEALKEAILRALQEGDLFPEGSLNEELRKMLDRSDAMNNQEIKDLLDKLVER
                     LSQEGFINPQQPPQITPPNQATARGSVGSPTERNTEARFEITDKTIDFLGFKTLRDLL
                     GSLGRSSFGRHDTRDLATGVEASGVSRQYEFGDTLNLDVSETLFNAVRRDGAKVPIDI
                     SYKDLMVHQCEYQSSCATVVMLDCSHSMILYGEDRFTPAKRVAMALSHLIRTQYPGDT
                     LNLVLFHDSAEEVPLNELARVQVGPFYTNTREGLRMAQRILLRQRKDMRQIIMITDGK
                     PSALTLEDGRIYKNAFGLDPFVVSETLEEVNKCKRANIMINTFMLATDYSLVNFVQKI
                     TQMCRGKAYFTTPHNLGQYLLMDFMQRKTRHIH"
     gene            4069..4518
                     /locus_tag="VFR42_01560"
     CDS             4069..4518
                     /locus_tag="VFR42_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU0367864.1"
                     /translation="MSDTNKNAVRRLFDEVWNKGQQQIADELFAPTYTHHDSSTPDAG
                     RGPESEKKRVAIYRNAFPDLRLTVEDLTAEGETVTARWSCHGTHKGDLNGIAPTGKQF
                     TISGISVARFTNGKMVEGWVNWDALGMLQQLGIVQELGKAKTAAAVR"
BASE COUNT         1020 a         1656 c         1089 g          925 t
ORIGIN      
        1 cctatctgtt attctttgcc tatagtcgga acgcgcctat gtctaatgtt cgcctgccgc
       61 tgtttccctt gaacgttgtt cttcttcctg gctctgatct tcccttgcat attttcgaac
      121 cgcgctatcg ccaaatggtc cgcgaatgtc tcgacacgca aacgcctttc ggaattttgc
      181 tcgctcttcc taacggcatc gccggcaccg gctgcaccgc cgaaatcctg gaagtcacca
      241 agcgttatcc cgatggccgc tgcgacatcc tcaccgtcgg ccgctcgccc ttccgcgtcg
      301 tccacctttt caacgacgag ccgctcctcc gcggcgaagt ggacttcctg gaagatcgcc
      361 cctcacccat aaactcccgc atttgccgcg aactcaacga tctctacgaa acttgccaca
      421 ccctgatccg cggagactac ccgcgcgacc tgtgctccac tcaggattcc gatatttcct
      481 atgaggtcgc tgccaaactt cccatggaac tgctctggaa gcagtgtatt ctcgagctgc
      541 gttctgaagc cgaacgccag gaacgcctgg tcgcgtatct tcgcgagtgg gcaccgcatc
      601 tagaaaagac cacgaccttg cgtgcgcgag ccgctaacgg cttaaattga actgagtttt
      661 gcgcgacaaa acgcttttgc cacgtcagcg catctaacaa gtggttcgcc ggatttgcat
      721 tgcgaacagg ggcccaatga ccattgctac ttcaacgcat tcttccggag gccgcgccag
      781 acccgcgaca ctcggtgaac tgcgccgcat cccggacttc gacgcccgcg ccgcatcccg
      841 ctgcgtgaaa gacgaaatcc gcaccaactt gctgcgcgcc atcgaacgcg gtgaaactct
      901 tttccccggc gtgcagggct atgaagacac cgtcatcccg caaatcgtca atgccctgct
      961 ctcgcgccac aacttcatcc ttctcggttt gcgcggacag gccaaaagcc gcatcctccg
     1021 cggcctgatc aatcttctcg ataccgaaat tcccgtaatc gccggctgcg aaatcaacga
     1081 tgatccgctc aagcccatct gccgcgcctg ccgcgaacgc atcgccgccg aaggcgacaa
     1141 cacgccaatt gcctggatgc cgcgcgacct tcgttacgta gaaaaactcg ccacgcccga
     1201 cgtcaccatc gccgacatca tcggcgacgt tgatcccatt cgcgctgcac gcggtggccg
     1261 cgatctctcc gacgaactca ccatccacta cggtctgctc ccccgggcca atcgcggcat
     1321 cttcgccgtc aacgaactcc ccgacctagc cggaaaaatt caagtaggcc ttttcaacat
     1381 catgcaagag ggcgacattc agattaaggg ctacccgctg cgcctgccac tcgatgtgct
     1441 gctcgttttt tccgccaatc ccgaagacta caccgcgcgc ggcaaaatca tcactccgct
     1501 caaagaccgc atcggctccg aaattcgcac ccactatccc gccactctcg aagaaggcgt
     1561 ggccatcacc acgcaagaag cctggaccgg ccgcgactcc gcctatcgcg tcgaagttcc
     1621 cgattacctc cgcgaactcg tcgaagaaat cgcgttccag gcccgcgacg acaaacgcgt
     1681 ggacaagcgt tccggcgtca gccagcgcct tcccattagc gttgtcgaat ccgtcgtcag
     1741 cagcgccgaa cagcgcgccg cccgcaccgg cgaaaaactc accgtcgcgc gcgtcgccga
     1801 tgtctacgcc gccatgcctt ccatcaccgg caagctcgag ctcgaatacg aaggcgaact
     1861 caaaggcgcc gacactgtcg cccgcgaact catccgcacc gccagcggcc gcattttctc
     1921 caagcgcttt tccgacgtga acttccagcc catcatccaa tggtttgaac taggcggcga
     1981 actcaaacta gccgagctcg cttccaccgc cgagcgctac aagcagctcg ccaacatcaa
     2041 aaacctgatg gatcacgtag accgactggg cgccgacaaa aaagatcagg cacacgccgc
     2101 cgccgccgcc gaaatgattc tcgaaggcct ctgggcccat cgccgcatcg gccgcagcga
     2161 agaacgcggc ttcttcgccg aaaagccgcg ccagcccgaa ccgcgcgaga gtggcccgcc
     2221 ccgccccccg cgccggagct acaactgatg cccagccgca ccagtggcac aggcttcagc
     2281 gtgtgctctt acgagcaagg cggtgctgtg ccgcctgctt cgatctcgga ccggcgtcaa
     2341 agcgagagtg tcatgctgac ttgcgcgaag cgctacgaag gatcttgctc ttgtttttgt
     2401 catcgcgagc gaagcgaggg atctgctttt tccgagcatt ttggaacttt aaacttttga
     2461 actcttaact gccttttatg aaattcacca aattcaccaa atacaccggc gaacccgccg
     2521 actccatcga cctcgaagaa ctcatcaagc gcctcggcga cttcttcctg caatccggtt
     2581 tcgaatcgca atactacggc atcactgaat tcgatccaca acgctcgctc gaagccctga
     2641 aagaagccat cctccgcgcc cttcaggaag gcgacctttt ccccgaaggc tccctcaacg
     2701 aagagctccg aaaaatgctc gaccgctccg acgccatgaa caatcaggaa atcaaggacc
     2761 tgctcgacaa gctcgtcgag cgcctctccc aggaaggttt catcaatccc caacagccgc
     2821 cgcaaatcac tccgcccaat caagccaccg cgcgcggctc cgtcggctct cccacggagc
     2881 gcaacaccga agctcgcttt gaaatcaccg acaagaccat cgacttcctc ggcttcaaaa
     2941 cgctccgcga tcttctcggt tccctcggcc gcagcagctt cggccgccac gacactcgcg
     3001 acctcgccac cggtgtcgaa gccagcggcg tctcccgcca gtacgaattc ggcgacacgc
     3061 tcaatctcga cgtcagcgaa actctcttca acgccgtccg ccgcgacggc gccaaagtcc
     3121 ccatcgacat cagttacaag gacctcatgg tccaccagtg cgaataccaa tcctcttgcg
     3181 ccactgtcgt catgctcgat tgttcgcaca gcatgattct ctacggcgaa gaccgcttca
     3241 ctcccgccaa gcgcgtcgcc atggcgctct cgcacctcat tcgcacgcaa tatccgggcg
     3301 acacgctcaa cctcgtcctc ttccacgact ccgccgaaga agttcctctc aacgaactcg
     3361 cccgcgtcca ggtgggtccg ttctacacca atacacgcga gggcctgcgc atggcccagc
     3421 gcattctgct ccgtcagcgc aaagacatgc gccagatcat catgattacc gacggcaaac
     3481 cctccgccct caccctcgaa gacggccgca tctacaaaaa cgctttcggc ctcgaccctt
     3541 tcgtcgtcag cgagaccctc gaagaagtca acaaatgcaa gcgcgccaac atcatgatca
     3601 acaccttcat gctggccacc gactattccc ttgtaaattt cgttcagaaa atcacccaaa
     3661 tgtgccgcgg caaagcctac ttcaccactc cccacaacct cggccaatac ctcctcatgg
     3721 atttcatgca gcgcaaaacg cgccacatcc attagccgct tcacaaaatt gcttctcgat
     3781 tgtccttgac atcgtcatcc gatgagacca cgctcactca gtggtttcgc cgtttgaggc
     3841 gaaactccag cttcgcagtc taaacgcagt tttctttaag gccaagcttc gcaggcgcta
     3901 gcagggcaca aacccctcga tcctttttcg ttccccctac gcagcctggc ttcctgtccg
     3961 acgtacaggt cgacgcacca aacacgccag cggtcacaag ccataccact caagcccgac
     4021 tcgcttcgcg cgggcggaat ctgaattttc taagctagga gaaatactat gtctgatacc
     4081 aacaagaatg ctgtccgtcg tctttttgac gaagtttgga acaagggtca acaacaaatt
     4141 gccgatgaac tcttcgctcc aacctacacc catcacgact cttctacgcc tgacgccggc
     4201 cgcggccccg aaagcgaaaa aaagcgtgtt gccatctacc gcaatgcgtt ccccgacctt
     4261 cgcttgaccg tcgaggatct caccgccgag ggcgaaaccg tcaccgcccg ttggtcctgc
     4321 cacgggacac acaagggcga tctcaacggc attgctccca ccggtaagca gttcaccatt
     4381 tccggaatca gcgttgcccg cttcaccaac ggcaaaatgg tcgaaggctg ggtcaattgg
     4441 gacgctctcg gcatgctgca gcagctcggc atcgtccagg aactcggcaa agccaaaacc
     4501 gccgctgccg ttcgctaacc ggttcctcga gcgcaaacca cggaaggccc gatctcacag
     4561 tcgggccttc tgttttcctt tttccgccga gttgtgcttc cagccacgcc tccggacggg
     4621 ccctgacaac cgtaccgggc acttattgtg gctaggttta ggtgtttgtc ttggctgccc
     4681 cgtcctcggt
//
LOCUS       DASTPM010000087         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1213743, whole genome shotgun sequence.
ACCESSION   DASTPM010000087 DASTPM010000000
VERSION     DASTPM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213743"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFR42_01565"
     CDS             <1..977
                     /locus_tag="VFR42_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0367865.1"
                     /translation="EALEADALHFSTDVWSTIVVIGGIGLVWAGERWNLPWLVYADAL
                     AGLAVAGVILWVGSRLGKRTLDALLDAVPEGLQEEIAGAVRKMDGVLSVERVRLRRAG
                     NRYFVDATVSVPRTASLEQVHSWSDAIEKRVGEIVPSDVVVHAEPRAPQGEPFFDSIR
                     AAAQRLGLAIHDLSAQQNKGQLFVELHLEVDPHLSLKDAHAQATQLEDAIRELRAEPT
                     EVNIHIEPLGTAITPTEESAGDLRDLGRTMEAFLHQLSSEYYELLNAHNVRVRQVEHH
                     ILASCHCTMRSELPITQIHDVTAALEDRVKERFPQVYRVTIHPEPLEE"
     gene            complement(1009..>2457)
                     /locus_tag="VFR42_01570"
     CDS             complement(1009..>2457)
                     /locus_tag="VFR42_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367866.1"
                     /translation="MKRVFSHTLTALITGFFFRLFFLLRLPSTAGDTALYETLSLNWV
                     KHSIYGMPVDGVLAPVDIRMPGYPAYLALVQAFTNLNGEAARFWVMLGQVFLDLACCI
                     FIAELASHCSGRKEDTRKVYLIALWLAALCPFTANYTAVPLTETFTMFWTALALTTLV
                     PMVNPEQATGELRKWGRFPQRSEFLKNAFWAGVCVGFATLFRPESPLLLVACLPVVLW
                     MMFFRGQFARGLWAAGLACVACFLVLLPWTVRNAVTLHEVQPLTPRYTTLPGERAPVG
                     FMSWEKTWLYRFREVFLVSWKMNDETIQIDDIPARAFDSPEERQHVAAILEQYNQDSN
                     LTAEQDAQFASIAQGRTERHPLRTYVWIPLQRVVTLWFTPRIEQLPISGSIFPLAHTW
                     QTDRLDMSATLALFFINIFYVVCALWGAVRMWRCAPETRAAVALLALFVLVRTAFLTT
                     VETPEPRYVLLCFPAVIALAAHGLLRGKLAQS"
BASE COUNT          542 a          763 c          715 g          437 t
ORIGIN      
        1 gcgaagccct tgaggccgac gcgctgcatt tttccaccga cgtgtggagc accatcgtcg
       61 tcatcggtgg cattggcctc gtatgggccg gcgaaagatg gaatttgccg tggctcgtct
      121 atgccgatgc gcttgctggc ctcgcggtcg cgggtgtcat cttgtgggta ggctcgcggc
      181 tgggtaagcg cacgctggac gccttgctcg acgcagtgcc tgaagggcta caagaagaga
      241 tcgcgggtgc cgtgagaaag atggacggcg tgctttccgt ggaacgggta cgtttgcgcc
      301 gcgcgggcaa tcgctatttt gtagatgcca cagtgagcgt gccgcgcacc gccagcctcg
      361 aacaggtgca ctcctggagc gacgctatcg aaaagcgcgt cggcgaaata gtgccttccg
      421 acgtcgtcgt ccacgccgag ccgcgtgcgc cgcaaggcga accctttttc gattccattc
      481 gcgcggcggc ccagcgcctg ggactggcca tccacgacct ttcggcccaa caaaacaaag
      541 gacagctgtt cgtcgaactc cacctcgaag tggacccgca cctcagtctg aaggacgctc
      601 acgcgcaggc tacgcagctg gaagacgcca ttcgcgagtt gcgcgccgag ccgaccgaag
      661 tcaacatcca catcgagccg ctgggaaccg ccatcactcc aacggaagaa agtgccgggg
      721 acttgcggga cctcggccgc accatggaag cctttttgca ccagctctcc tcggaatatt
      781 acgaactgct gaacgctcac aacgtgcgcg tgcgccaggt agagcaccac attcttgcgt
      841 cttgccattg cacgatgcga agcgaactgc cgattacgca aattcatgac gtcaccgcgg
      901 cgctggaaga ccgcgtgaag gaacgctttc cccaggttta tcgcgtgacg attcatccgg
      961 agccgctcga ggaataacga gtcaaacgat gctcgcccta cgacttgttc aggactgtgc
     1021 cagtttgcct cgcaggaggc catgcgccgc gagcgcgatc actgccggga aacacagcaa
     1081 aacataccgc ggctcaggtg tttccacggt cgtcagaaac gcggtgcgga ccagaacaaa
     1141 tagagccaac agggccactg cggcgcgggt ctccggcgcg catcgccaca tccgtacagc
     1201 tccccacagc gcgcagacca cgtagaaaat gtttatgaaa aagagagcca gggtggccga
     1261 catatccagc cggtcggttt gccaggtgtg cgccagcgga aaaatcgaac cggagatggg
     1321 cagctgttcg atgcgtggcg taaaccacag agtgaccaca cgctgcagtg gaatccacac
     1381 atatgtccgt aatggatgcc gctccgttcg gccctgcgcg atcgacgcaa attgtgcatc
     1441 ctgctccgcc gtcaggttcg agtcttgatt gtactgctcc agaattgccg ccacgtgctg
     1501 ccgctcttcc ggggaatcaa aggcacgcgc cggaatgtcg tcgatctgga tcgtttcatc
     1561 attcatcttc cacgaaacca ggaaaacttc gcggaagcgg tacagccagg ttttctccca
     1621 gctcatgaac cccacgggcg cccgttcacc gggcagcgtt gtataccgcg gcgtcagcgg
     1681 ctggacttca tgcaaagtga cggcattccg gaccgtccac ggcagcagca ccaggaaaca
     1741 cgcgacacat gccagccccg ccgcccacaa accacgcgcg aattggccgc ggaagaacat
     1801 catccacaaa accacgggca ggcacgcgac cagcagcaac ggcgactccg gccggaacaa
     1861 tgttgcaaat ccgacgcaga cgcccgccca aaatgcgttc ttcagaaatt ctgagcgttg
     1921 cggaaaccgg ccccacttgc gcagctcgcc ggttgcttgc tcgggattca ccatcggcac
     1981 caatgtggtc aaagccagtg cggtccagaa catggtgaaa gtttcggtca gcggaacggc
     2041 agtatagttc gccgtaaacg ggcagagcgc cgccagccac agcgcgatga ggtacacctt
     2101 tctggtgtct tccttgcgtc cggagcaatg cgaggccagc tccgcgatga atatgcagca
     2161 tgccaggtcc agaaaaacct gccccagcat cacccaaaag cgtgccgcct ctccgtttag
     2221 gttggtgaat gcctgcacca gtgccaggta cgcgggatag ccgggcatgc ggatgtccac
     2281 cggcgccagc acgccgtcca cgggcatgcc gtaaatgctg tgcttcaccc aattcagcga
     2341 cagcgtttcg tagagagcgg tgtctcccgc cgtgctcggc aagcgcaaca aaaaaaacag
     2401 cctgaagaag aatccggtta tcagtgcggt cagggtatgc gagaaaactc gcttcat
//
LOCUS       DASTPM010000088         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1156291, whole genome shotgun sequence.
ACCESSION   DASTPM010000088 DASTPM010000000
VERSION     DASTPM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156291"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="VFR42_01575"
     CDS             <1..1545
                     /locus_tag="VFR42_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0367867.1"
                     /translation="AGLMLSRASTRQKEMSVRLALGAGRGRVTRQLLAESVMLSLAGA
                     AIGILIAYWGANSLAAFVAANRYSPLYLNASPDLTVLAFTVAVALLTGILFGLAPALR
                     GTSVNVAAMLKENAASAAAVVSGKLPKFALGNILVVAQVTLSVIVLVGAGLVVRSLAH
                     LKGVDPGFDTHNILQFGVDPTLTGYYKENQIPPLYRELQTRLSALPGVVSVSYSSDTL
                     LDGSLWTSDVKIQGRADKATVETQMLAVGPDFFATMRIPAVTGSLLDQADVESSLEVA
                     VVNQAFVKKFLENREPLGLHFGGEDPKDPQYEIIGVVGDTKYSDLRLAVEPTAFKPLK
                     KGGVHFAVRTASHPLSVLPAARRVVADLDNNLPLFDVRTQTERIERLLFNERLIARLA
                     ALFALLALLLACLGLYGLLAYEVAQRTREIGIRTALGAQKRDVLRLVGTQGLALVVSG
                     IAFGVLAALVITRFLGSLLYEVRPTDPVTLLAVCILFLLVGVLACWIPARRAALVDPM
                     SALRCE"
     gene            1644..>2199
                     /locus_tag="VFR42_01580"
     CDS             1644..>2199
                     /locus_tag="VFR42_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEU0367868.1"
                     /translation="MKRRDNLLDNLGQEIREHIEIETQQNITRGMAPDDARRAALIKF
                     GNPTLVAEDTRAVWRHLWLDELVQDLRFGLRMLLKSPGFTVVAVLTLALGIGANTAIF
                     SVVNALLLQALPYPHPERLGTIYTRIAGSANSDERHNLNGEQLELLRANVPSLISAVS
                     SSGISGVNFRSGSHVQYLQSARVSA"
BASE COUNT          401 a          713 c          606 g          479 t
ORIGIN      
        1 gccgggttga tgctttctcg cgcgtccact cgtcagaaag aaatgagcgt gcggctggcc
       61 ttgggcgccg gacgcggccg cgtcacccgc cagctcctgg ccgaaagcgt gatgctgtcc
      121 ctcgccggag ctgccatcgg aattctgatc gcctattggg gagccaattc tcttgccgcc
      181 ttcgtcgctg ccaatcgcta ctctccgctg tacctgaacg ccagccccga cctcaccgtc
      241 ctggccttca cggttgctgt tgcgctgctt accgggattc tctttggtct cgcgcccgcc
      301 ctgcgcggca ccagcgtcaa tgtcgcggcc atgctcaagg aaaatgcggc tagcgcggcc
      361 gcggtagtgt ccggcaaatt acctaagttt gccttgggaa acatcctggt ggttgcgcaa
      421 gtgactcttt cggtgattgt tctggtcggc gccggactgg tggtccgctc gctggcccat
      481 ctaaagggag tggatcccgg attcgacacc cataacattc ttcagttcgg agtggatcca
      541 acccttaccg gctactacaa agaaaaccaa ataccgccgc tctaccgcga attgcaaacc
      601 cgcctgagcg cattgcccgg cgtggtttcc gtcagctatt cctcagatac gctcctggac
      661 ggcagcctgt ggacttcaga tgtaaagata cagggccgcg ccgacaaggc gacggtcgaa
      721 acgcagatgc tggcggttgg cccggacttc tttgccacca tgcggattcc cgcggtgacc
      781 ggcagtctgc tcgatcaagc cgacgtcgaa tcaagccttg aagttgccgt ggtaaaccag
      841 gcctttgtga aaaagttcct ggagaaccgc gagccgctgg gccttcattt tggcggtgaa
      901 gatcccaaag acccgcagta tgaaatcatc ggagttgttg gcgacacaaa atacagcgac
      961 ttgcgactcg ccgtggaacc gaccgctttc aaaccgctca aaaaaggcgg cgtacatttt
     1021 gccgtccgga cagcttccca cccactctcg gtgctaccgg cggctcgccg cgtggtcgcc
     1081 gacttggaca acaacctgcc tctgttcgac gtgcgcacgc agactgaacg catcgagagg
     1141 ctgctcttca acgaacgttt gatcgcgcgt ctggccgccc tgttcgcttt gctcgccttg
     1201 ctgctggcct gcctcggcct ttacggcctg ctggcttatg aagtggcgca gcgcactcgc
     1261 gaaatcggca ttcgcaccgc cctcggcgcg caaaaacgcg atgtactccg gttggtcggt
     1321 acacagggac ttgcattggt tgtcagcggc atcgccttcg gagtcctcgc cgcgctcgtg
     1381 attacgcgtt ttctcggcag cctgctctac gaagtgcgtc ccaccgatcc agtcactctt
     1441 ctggcagtct gcattctgtt cctgctggtt ggcgtgctgg cgtgctggat tcccgcgcgc
     1501 agggcagcgc tcgtcgaccc gatgtccgcc ctgcgctgcg aataaggcgc gtgcgaaacg
     1561 ccacgactca ccaacgccta gatcccgcct cctttcacgg gatgaatctc gtttccgctt
     1621 agttggcagc ggaggacaac tccatgaaac gtcgtgacaa tctccttgat aacctcggcc
     1681 aggaaatccg cgagcacatc gaaatcgaaa cccagcaaaa catcacccgc ggcatggctc
     1741 ccgatgacgc tcgccgcgca gcgctgatca aatttggcaa cccaacgctc gtcgccgaag
     1801 acactcgcgc tgtatggcgt cacctttggc tcgacgagct tgtgcaggac ctccgcttcg
     1861 ggctgcgcat gctcctcaag agccccggct ttaccgttgt cgcggtgctc acactggcgc
     1921 tcggcatcgg cgccaacact gcgatcttct cggtcgtgaa cgctttgctg ctacaagcct
     1981 tgccctatcc gcatccagag cgtttgggaa cgatttacac gcgtatagca ggctcagcga
     2041 actccgatga gcgtcacaac ctgaacggcg agcagttgga gcttctccgc gccaatgtcc
     2101 cctctctgat ctccgctgtt tcaagcagtg gaatttccgg cgtcaacttt cggtcgggtt
     2161 cccatgtgca gtaccttcag tcagcccgcg tttccgcac
//
LOCUS       DASTPM010000089         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_378060, whole genome shotgun sequence.
ACCESSION   DASTPM010000089 DASTPM010000000
VERSION     DASTPM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378060"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1148)
                     /locus_tag="VFR42_01585"
     CDS             complement(204..1148)
                     /locus_tag="VFR42_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367869.1"
                     /translation="MNRRHALVAYILVACTLVLALISRAAETRNGSWTIVRSEEAGKV
                     SFELIYHNKRNNSNHQSDWPVTDFKGVDFSKPGKQDVKFVIARDAGKFDCEGYLNNGE
                     GAGVFHFNADPQYPSEMSRLGFSGIDDEKQFAMAIFDVSLAYAKEMKSRNLKGLDADK
                     LIAFRIFNVTQNFIDDLRKSGLPATDADKLVAFRIHGVSPEMVAYLHKSGYQPDEDTL
                     VAMRIHGVSPEYMQQLKTDGYDHIDLQKLIAFRIHGVSPDFIEKLATLGYRHPEPDQL
                     VAMRIHGVSPEFISQMQSRGMKNLTVDQLVNLRIHGID"
     gene            complement(1366..3411)
                     /locus_tag="VFR42_01590"
     CDS             complement(1366..3411)
                     /locus_tag="VFR42_01590"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU0367870.1"
                     /translation="MNAFSEWLAPDVLRNLGLALSHFIWQGAAIAALAAAGIAFARKA
                     SVRYTIGVTALAFMFAAPVITFLVLNNSTIAPTPLATSDVSVIVAGDVPSLAYHGSAR
                     SAAPAVPVNMTSMNWLVAIWFAGVLLFSLRTAGGFFLVARLRRRDSAPVATELLSLCR
                     DLQQRLGIMRTVRYCESLHLDAPAVLGWFRPVVFLPVTALTGLSEGQLRAVIAHELAH
                     IRRFDAFVNLFQVAVETLLFYHPAVWWLSTRVRAERENCCDDVALSVFGNPAEYARAL
                     ALMEQWRVAPSFVMAANRGPLASRVARLLGLGEKGSSLRNAGVAFGILCVATALIAGN
                     ALFGMVRPVSARALFSLQTPAVQISSGEPVITITAPRPAPQAKPAQKPSPAQAAQPEQ
                     TPSPKSQSYIDGLRAEGFDNLSADDLIAMKVQGITPQYIHELRAEGLKPSADELVSMK
                     VQGITPDYIRQIRAMKLNSDIDSLIGMKVQGITPDYVAQIHKLGFNSDTDQLIAMKVQ
                     GITPEYVDQMRKLGFQPDGDQVIAMKVQGITPEYVDQMRKLGFQPDGDQLIGMKVQGI
                     GPEYVRALNDLGIHPDADDLIGMKVQGINADYVRSIRSTGINPDKDEWIALKVQGVTA
                     DYIKGLQAAGFRPDVDEIVGSKVQGITPQFIELAKSHGFKNLDLDKLIQLKHAGVLE"
     gene            complement(3411..3797)
                     /locus_tag="VFR42_01595"
     CDS             complement(3411..3797)
                     /locus_tag="VFR42_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEU0367871.1"
                     /translation="MKDASPQKPTAAELQILQVLWERGPSTVREVHEALQEEKALGYT
                     TVLKLMQIMTTKGLVRRDENQRAHVYEAQQPAEKTKRQFAADMLQRVFDGSARELMLH
                     ALATQRSTRQEIEELRNLLDEHERNL"
     gene            3930..4757
                     /locus_tag="VFR42_01600"
     CDS             3930..4757
                     /locus_tag="VFR42_01600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0367872.1"
                     /translation="MKNAKRRGRRNAGNGGPKVGLARALSKLGYCSRSVAAKLIGAGR
                     VRLNGSLTRNPETAVRMGTDRIEVDGVRVRLLEKMYWMLNKPRGVVTTAGDEQGRETV
                     YSGLPDGLPWMGPVGRLDKASEGLLLFTNDSEWAARITAPESHLDKTYHVQIAAVADE
                     VLLQKLREGVPAADGERLRVKTARIIRGGEKNTWIEIMLDEGKNRQIRRMVEGCDVEV
                     LRLIRVAIGRLELGSLGKGAARELSRQEKLALDRELGLLSQVSAVQGRNRIATANHQ"
     gene            4928..>5189
                     /locus_tag="VFR42_01605"
     CDS             4928..>5189
                     /locus_tag="VFR42_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367873.1"
                     /translation="MRTLGICWIVYGIFRLAMGAGAALCAPTATVMFGALLTRVADPF
                     TWMGFFHVGYVCWIGLCFVCGILGIVGGLALMRSPNGGRGLLV"
BASE COUNT         1113 a         1301 c         1690 g         1085 t
ORIGIN      
        1 tttctacgac tttcaaacgc gactagtccg gcagatcagt ctattggacc ggagcccttt
       61 ctggttgtcc gcgtcagcga atggcaaaga agtgttcttc aatcaggcag agcgcgatga
      121 gagcagcatt gtgctggtga aggcaaaatg aaaatgggac caaggcggcg acataaagcc
      181 gccgcgacag gcgcgtcgtg acttcagtca atgccatgga tgcggagatt tacgagctgg
      241 tcgacggtca aatttttcat gccgcgcgat tgcatctggc tgatgaattc cggcgagaca
      301 ccatggatgc gcatggcgac cagttgatcc ggctccgggt gcctgtagcc gagcgtcgcc
      361 aatttttcga tgaagtcggg cgaaacaccg tgaatgcgga acgcgatcaa cttttgcaga
      421 tcgatgtgat cgtagccgtc ggtcttgagc tgttgcatgt attcgggtga cacaccgtga
      481 atgcgcatgg cgacaagggt gtcttcgtcg ggctggtaac cggatttgtg gagatacgcg
      541 accatttccg gtgagacgcc gtgaatgcgg aacgccacaa gtttgtcggc atcggttgcg
      601 ggcaggccgg acttgcgcag gtcgtcgata aaattctgcg tgacgttgaa gatgcggaag
      661 gcgatgagct tgtcggcgtc tagtcctttg aggttgcgcg acttcatttc tttcgcatag
      721 gccagcgaga cgtcgaagat ggccatggcg aattgcttct cgtcgtcgat gccgctgaag
      781 ccgagcctgc tcatctcgga cgggtattgc gggtcagcgt tgaaatgaaa gacgccggcg
      841 ccctcaccgt tattcagata accttcgcaa tcgaacttgc cggcatcgcg agcgatcaca
      901 aacttgacgt cctgcttgcc gggttttgag aagtcgacac ccttgaaatc ggtcaccggc
      961 cagtcggatt gatgattcga attgttgcgc ttgttgtgat agatgagttc gaaggagacc
     1021 ttgccggcct cttcggaacg gacgatggtc cagcttccgt tgcgagtttc ggcagcgcga
     1081 gagatgagcg caagcaccag ggtgcaagcg accaggatgt aagcgacgag tgcgtgacgg
     1141 cgattcatgg tggcaatgtc ctccatatag gtacctctaa aagcgaactt cgagaaccgc
     1201 agagagattc ctcgctacgc tcggaatgac gacggcctct ttgttccgcc atcgcgattc
     1261 tcgtcggcgc agctcgaacg aacggagcga acaaccgggc acacgcagag ggcagcgcgc
     1321 gaacgacacg gttcaggcgc gacactcacc gagaacgaac tttgattact ccagaactcc
     1381 tgcgtgcttg agctggatca gcttgtctaa atcgagattc ttgaagccgt ggctcttcgc
     1441 cagttcgatg aactgcggcg ttattccctg cactttcgat ccgacaattt catccacgtc
     1501 aggcctgaag ccagcggctt gcagcccttt gatgtagtcg gcagtgacgc cttgtacttt
     1561 gagtgcgatc cattcgtctt tgtcgggatt gattccggtg gagcgaatgc tgcgcacgta
     1621 gtcggcgttg atgccttgca ccttcatgcc gatcagatcg tcggcgtcgg gatgtatccc
     1681 tagatcattc agcgcgcgaa cgtattcggg accgatgcct tgcactttca tgccgataag
     1741 ttgatcgccg tcaggctgaa agccgagctt gcgcatctgg tccacatatt cgggcgtgat
     1801 gccttgcacc ttcatggcaa tgacctgatc gccgtcgggc tgaaaaccaa gcttgcgcat
     1861 ctggtcgacg tattcgggcg tgatgccttg cactttcatg gcaatgagtt ggtcggtgtc
     1921 ggaattgaag ccaagtttat ggatctgagc gacgtaatcc ggtgtgattc cctgcacttt
     1981 catgccgatc agtgaatcga tgtcgctgtt gagcttcatg gcgcgaatct ggcggatgta
     2041 gtcaggggtg attccctgga ccttcatgga taccaactcg tcggcggaag gcttgaggcc
     2101 ttctgcgcga agttcgtgga tgtattgcgg cgtaatgccc tgcactttca tagcgatcag
     2161 atcatcggcg gaaagattgt caaagccttc ggccctaagg ccgtcgatgt aggattggga
     2221 tttcggcgat ggagtctgtt cgggctgagc cgcttgtgct ggagaaggct tttgcgcggg
     2281 cttggcctgg ggcgccggac gcggcgcggt gatggtgata accggttcac cggaggaaat
     2341 ttgtaccgcc ggtgtttgca gcgagaacaa ggcgcgcgcg gaaaccggac gaaccatgcc
     2401 gaagagggcg tttccagcga ttagcgcggt ggcgacgcag agaatcccga aggcgacgcc
     2461 cgcgttgcgc agactgcttc ccttttcgcc gaggccgagt aggcgagcga cgcgggaggc
     2521 cagcggaccg cgattggcgg ccataacaaa agacggagcg acccgccact gttccatgag
     2581 agcgagagcg cgcgcgtatt cggcgggatt gccgaaaaca gagagcgcga cgtcgtcgca
     2641 gcaattttcg cgttcggcgc gaacacgcgt gctgagccac cacaccgccg gatgatagaa
     2701 gaggagggtt tcgacggcga cctggaaaag attgacgaag gcgtcgaagc gacggatatg
     2761 cgccagctca tgagcaatga cggcgcgaag ttgcccttcg ctcaggccgg tgagcgcggt
     2821 taccggaaga aagacgaccg ggcggaacca gccgaggacg gcaggcgcgt ccaaatgaag
     2881 ggactcgcaa taacgcacgg tccgcatgat gcccagacgc tgctgcagat cgcggcaaag
     2941 cgagaggagt tcggtggcga cgggagcgga gtcgcggcgg cgcaggcgag cgacgaggaa
     3001 aaaaccgccg gcggtgcgaa ggctgaagag cagcacgcca gcaaaccata tggcgacaag
     3061 ccagttcatg ctggtcatgt tcaccggaac ggccggagcg gcggaacgcg ctgatccgtg
     3121 gtacgccagg ctgggaacat cgccggcgac tatcaccgag acgtcggatg tcgcaagtgg
     3181 agtcggagca atcgtcgagt tgttaaggac tagaaaagtg attacgggcg ctgcgaacat
     3241 gaacgccagc gcggtgactc cgatcgtgta gcggacggac gctttacgcg caaacgcgat
     3301 gcctgcggct gccagggccg cgatggccgc gccctgccag atgaaatgcg aaagggccaa
     3361 gcccagattg cgtagaacgt cgggagcgag ccactcagag aaggcgttca tcacagattc
     3421 ctttcgtgtt cgtcgagcag gttgcgaagt tcttcaattt cttgccgggt gctgcgctgc
     3481 gtggctagcg cgtgcaacat caactcgcgg gcggagccgt caaaaacgcg ctggagcatg
     3541 tccgcggcga attggcgctt ggtcttttcg gcgggctgct gcgcttcata gacgtgggcg
     3601 cgctggtttt cgtcacgccg gacgagccct tttgtggtca tgatctgcat gagtttcagg
     3661 accgtggtat agccgagagc cttctcttcc tggagcgctt cgtggacttc gcgaaccgtg
     3721 gagggcccac gttcccagag cacttgcagg atttgcagct cggctgcagt cggtttttgt
     3781 ggagaagcgt ctttcatgag aggcaccgta ctacgaagga attcgtaatg tcaacgaaaa
     3841 tcttcgtagt tcgtttggac gcaggtttca gggtctggtg agcagcccga aacatataat
     3901 tatggagaga cggcaagccg aggagcggaa tgaagaacgc gaaaaggcga ggcaggcgaa
     3961 acgccggcaa tggtgggccg aaagtcggat tggcccgtgc cctctccaag ctgggatatt
     4021 gttcgcgcag cgtggcggcg aagttgattg gcgctggtcg agtccggctg aacggttcgc
     4081 taacaagaaa tccggaaacg gcagtgcgga tgggtactga ccgaatcgaa gtagacggcg
     4141 ttcgcgttcg gctattggaa aaaatgtatt ggatgctgaa caaaccgcgt ggcgtggtga
     4201 ctacggccgg cgatgagcag ggacgcgaga cggtttattc gggactgccg gacggactgc
     4261 cgtggatggg gcccgttggt cggttggaca aagcgagcga gggactgttg ctcttcacca
     4321 atgattcgga gtgggcggca cggattaccg cgccagagtc acatctggat aagacgtacc
     4381 atgtccagat cgccgcggtt gccgatgaag tgctgctgca aaaactgaga gaaggcgtgc
     4441 cagctgcgga cggtgagcgc ttgcgcgtga agacagctcg cataatccgc ggcggcgaga
     4501 agaacacctg gattgagatc atgctggacg agggcaagaa caggcagatc cggcggatgg
     4561 tagaaggctg cgacgtggaa gtgttgcggc tgattcgcgt ggcgattgga cggttggaac
     4621 tgggttcgtt gggaaaaggt gcggctcgtg aactgagccg gcaagaaaaa ctggctttag
     4681 atcgcgaact cggtctgctt agtcaggttt cggcggtaca gggaagaaat cgaatcgcta
     4741 cagcaaatca ccaatagcag gacagtagag cgctagggca aattgttttc ggagacggat
     4801 acaatccgtt ttcactagag ttcgaaggga aaggcggagt ttcccaagaa attgggacct
     4861 cagggctggg acgcctttcc cttcgtaacc agtaccactc gcggcaaact ccagaaactg
     4921 aatcaatatg agaactctcg ggatctgctg gatcgtctac gggatatttc gcctggctat
     4981 gggagcgggc gcagcgctgt gcgcgcctac tgcgacggtc atgtttggcg cgctgttgac
     5041 aagggtggcc gatccattca cctggatggg atttttccac gtagggtacg tctgctggat
     5101 tgggctgtgt tttgtctgcg gaattctggg aattgttggc gggctggcac tgatgcgaag
     5161 cccgaatggc ggcagaggct tgctggttg
//
LOCUS       DASTPM010000090         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_147200, whole genome shotgun sequence.
ACCESSION   DASTPM010000090 DASTPM010000000
VERSION     DASTPM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147200"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFR42_01610"
     CDS             complement(<1..1463)
                     /locus_tag="VFR42_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0367874.1"
                     /translation="MPIVVGLSVALLILHCYLNNRYDFFRDEFNYLACARHLAWGYVD
                     QPPLIPWLARLSLATLGDSLRAIRFLPALFMSLTIVLTAQIARELGGRAFALICAAAA
                     VIAAPSYLNDGAMLTTNCLEPLLWTACAYFAILAAKHDQPRYWLWFGVAAGIGMQEKY
                     SIAVLGFAVVVGLLLTEQRRVLFNKWIWLGGAAAFLIFLPNLLWNIHYDFPFIQLMHN
                     IKAEGRDVVLSPLEYFLQQCLLLQPIVAPLWIAGVCAFLFWRPFRAYRFLGIAYLVSL
                     TVFIVLHGKNYYLAPIYPMLLAAGSVLYEHALDRPKLHWLKPVSVAVILAGGAWIAPV
                     AMPVLPVERYLAYQEKLPFKVPRSEHSHMGAALPQHFADQFGWHEIVSEVVVAWNRIS
                     PAERDDCGIFAQDYGAAGSIDWYGPGYGLPASLSGHQTWFLWGPRGYSGNCLIVLDDS
                     RKRLEELFSHVEFVGNSPDNPYALEKNLPVFICKGKK"
     gene            complement(1641..2411)
                     /locus_tag="VFR42_01615"
     CDS             complement(1641..2411)
                     /locus_tag="VFR42_01615"
                     /inference="COORDINATES: protein motif:HMM:NF004836.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367875.1"
                     /translation="MSDTVRIGILGDFTPEFRSHHATNDALQHAASKLKLKVESEWVP
                     TPSLVEPEARKVLESFDGLWASPGSPYKSVDGMLKGIEFARTRDWPFLGTCGGFQYAV
                     IECARNVLGMKDADTAENNSGSKNIVIYPVACAVPDRKGNAPKLSGVVPEIRIRPGSY
                     LETFYGKNVIQEEFFCNFELNPDYEWCSIEAGFPIAARGPQGEVRAIESPTHRFFIAT
                     LFQPQLSSTAKKPHPLVIAFVQAAADWGRKKLDDSVLE"
BASE COUNT          594 a          679 c          801 g          452 t
ORIGIN      
        1 aatttcttac ccttacagat gaaaaccggc aaattctttt cgagagcgta gggattgtcg
       61 ggcgagttgc cgacgaactc cacgtggctg aacaattcct ccaggcgttt tcgagaatca
      121 tcgagcacga tgaggcaatt gccggagtaa ccacgcgggc cccagagaaa ccacgtctga
      181 tgcccgctca gcgaagccgg gagtccatag ccaggaccgt accagtcgat ggacccggcg
      241 gcgccgtagt cttgcgcgaa gatgccacag tcgtcgcgct cggcgggcga aatcctattc
      301 catgccacga caacttcgct cacgatctcg tgccagccaa actggtccgc gaagtgttgc
      361 ggcagggccg cccccatgtg gctgtgttcg ctgcgcggaa ctttgaaggg tagcttctcc
      421 tgataggcca agtaccgttc caccggaagc actggcatgg cgaccggggc gatccacgcg
      481 cccccagcga gaatgacagc caccgagacg ggtttcagcc aatgaagctt ggggcgatcc
      541 aatgcgtgtt cgtacagaac cgatcccgcg gccagcagca tcgggtaaat cggcgccaga
      601 tagtaattct tcccatgcag gacgatgaac acggtgaggc tcaccaggta cgcgatgccg
      661 agaaagcggt acgcacgaaa cggacgccag aacaggaagg cgcatacgcc ggcaatccaa
      721 agcggcgcta cgatcggctg caggagcagg cactgctgaa ggaagtactc gagcgggctc
      781 agcacgacgt ctcggccttc agccttgatg ttgtgcatca gttggatgaa cgggaaatcg
      841 taatgaatgt tccacagcag gttaggcaag aagatgagga aagcagcggc cccgcccagc
      901 caaatccact tgttgaacag gacgcggcgc tgctcggtaa gcaaaagtcc gacaaccacg
      961 gcgaatccga gcacggcgat cgaatatttt tcctgcatac cgatgcccgc ggcgacgcca
     1021 aaccacagcc agtagcgcgg ctgatcgtgc ttagcggcga gaatcgcgaa gtaggcgcac
     1081 gcggtccaga gcagcggttc gagacagttc gtggtgagca tggcgccgtc gttgagatac
     1141 gacggggcgg cgatcacagc ggcagccgcg cagatgagag cgaaggcgcg ccctccaagt
     1201 tcacgggcga tctgcgctgt gagcacaatg gtgagcgaca tgaacagggc gggcaggaag
     1261 cgaatcgcac gaagggagtc gcccagagtt gccagcgaaa ggcgcgcgag ccaggggatc
     1321 agcggcggtt gatccacgta tccccacgcg agatgtcggg cgcaagccag gtagttgaat
     1381 tcgtctcgga agaaatcgta gcgattgttg aggtagcaat gcaggatcag aagcgcgact
     1441 gacaggccca ctacgatcgg catgccgtcc gagaggaacc ggcttcttgg cggagacgtt
     1501 tgcatgaaag atggaaggat agcggaagcg ggaactagtt gcgagccgca gaaaatcctt
     1561 taccacagag gacacaaagg agcacagagg tttcccgcgc tgagcagtgc tctccgaaca
     1621 ggtgtgtcca ctccacaacg ctactccagc acactgtcgt cgagtttctt gcggccccag
     1681 tcggcggcgg cctgaacgaa ggcgataacg agtggatgcg gtttcttcgc ggtggagctg
     1741 agttggggct ggaagagtgt cgcgatgaag aagcggtgcg tgggtgactc aatcgcgcgg
     1801 acttcgccct gcggaccgcg ggcggcgatc gggaatccgg cttcgatgga gcaccactcg
     1861 tagtcgggat tcaactcgaa attgcagaag aattcttcct gaatgacgtt cttgccgtaa
     1921 aacgtttcca gataggatcc gggacggatt cggatttcgg gtacgacccc ggaaagtttg
     1981 ggagcattgc ccttacgatc cggtacagcg caggcgacgg ggtaaatcac gatgttcttc
     2041 gagccggaat tgttttcggc tgtgtcggcg tctttcatgc caagcacatt gcgggcgcac
     2101 tcgatgacgg cgtactgaaa tcccccacag gtgccgagga aaggccagtc gcgggtgcgc
     2161 gcgaattcaa tgccttttag catgccgtcc acactcttgt aggggctgcc cggcgaagcc
     2221 caaagaccgt caaaactttc aagaaccttg cgggcctcgg gctcgaccag agacggcgta
     2281 ggcacccact cagattcgac tttgagctta agtttcgagg cagcatgctg cagtgcgtcg
     2341 ttggtagcgt gatgcgaccg aaattcagga gtgaaatccc cgaggatgcc aatgcgaacg
     2401 gtgtcagaca cattgtcatt ctaaataacg acaggcgcga gcaaccgagg aatcgcgaaa
     2461 agcgggttag aaggaaggtg acaggggtgt ggcggatggt cgtttgtcgt tggtcgtggg
     2521 caaaaa
//
LOCUS       DASTPM010000091         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_118793, whole genome shotgun sequence.
ACCESSION   DASTPM010000091 DASTPM010000000
VERSION     DASTPM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118793"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFR42_01620"
     CDS             <1..711
                     /locus_tag="VFR42_01620"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0367876.1"
                     /translation="GWLRSVCNVFHGFAEHCFADELAHAAGKDPRDYVLALLGPDRII
                     PKSELAKDYTNYDGDYEKYPIDTARFRRVLEIATEKAGWGKKKNGNGFGMGLAIHRSF
                     LTYVATVVQVEMDTDGKVKIGRVDTAVDAGTIVNPEMALQQFEGAATFSTSLALFGEI
                     TAKDGVIQQTNFDTYPMARMNTSPRETYVHLVPSEAPPAGIGEPGVPPFTPALCNAIF
                     AATGKRVRQLPLSKLGLA"
     gene            861..>2666
                     /locus_tag="VFR42_01625"
     CDS             861..>2666
                     /locus_tag="VFR42_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0367877.1"
                     /translation="MTSRRYFLFAAVALLAAHAVVLKWLGDVWPGPLLSDTVQLLMGV
                     VVFCASLLAMRRSRTLGRLFWKLNAIAVLLWCAGQALGAYYGSYLNLPTRSLWFVDIF
                     YVAWPAPLVMCLFLDSEEEPEGANWRRLLDFGQVAIVFVLLFFYFAALSAQNSAWSTY
                     GLSAATDGLLTAAFLARAFSMRGEQVRKLFLGIGLFRAVALLTDVYFLAGLPEFGNGT
                     WFDLVWSAPWLIPLVTAISWTDAKQVQPGAFEASRRRPRMLLAHILPLIFPVLVVVMA
                     ASVAPTQLKIAALAVLLSLGTSYARLLLTHDALRRSTEALRNQHEMLNAIVEGTTAAT
                     YVKDLQGRYLLMNSAGATLLGKGVSEIIGKDDLALFSGESAREIIARDRETIESGVMQ
                     TYEQDISRDGVTRTLLATKGPLRDAQGRIAGVVGSSLDITERYRAIEALAESEERFRT
                     IFDGSPIGMAVIGTDGSIVASNSACRQMLNLKEEALRGVAIFDELTHPDDRAADAARF
                     QQLIRGEIEYDRREKRYILRDGRRVFADLHLYLVRDKRGAARYLIGISVDITERKSLE
                     TQLRQAQRMETIGRLAGGVAHDFNNLLMVIKGYCEL"
BASE COUNT          538 a          748 c          803 g          577 t
ORIGIN      
        1 ggctggctgc gcagcgtctg caatgttttt cacggcttcg cagaacactg tttcgccgac
       61 gagttggcgc atgcagccgg caaagacccg cgcgattacg tcctggcgct gctcgggccg
      121 gatcgcatca ttccgaagtc agaattggcg aaggactaca ccaattacga cggcgactac
      181 gaaaaatatc cgatcgacac cgcgcgcttc cgccgcgtcc tcgaaatcgc cacagaaaag
      241 gccggctggg gcaaaaagaa aaatggcaac ggcttcggca tgggcctggc cattcaccgc
      301 agcttcctga cctacgtcgc caccgtcgtc caggtggaaa tggacacgga cggcaaagta
      361 aaaatcggcc gcgtggatac cgccgtcgac gccggcacca tcgtcaatcc ggaaatggcg
      421 ttgcaacagt ttgagggcgc cgcaacattc agcaccagcc tcgcgttatt tggagaaatc
      481 accgcgaaag acggagtgat ccaacaaact aatttcgaca catatccgat ggcgcgcatg
      541 aacacatctc cgcgcgaaac ttacgtgcac ctggttccca gcgaagcgcc gccggccggt
      601 attggcgaac cgggcgttcc gccgttcact cccgcactct gcaatgcgat ttttgccgcc
      661 actggcaagc gcgttcgcca gctgccgctc agcaagctcg gtctggcctg acacgttttc
      721 gagttgcatg ggctgtgttc atgggcggct ccgcatacag gtctgccact acgtaaccaa
      781 ttgaagcaat tccgccagga acgtccttcc tgcaatttgt cctggaataa aatagaagaa
      841 cttctctctt cagggcacaa atgacgtcgc gccgttattt tctgttcgct gccgttgccc
      901 ttctcgccgc tcacgcagtt gttctgaagt ggctgggcga cgtttggccc ggcccgctcc
      961 tctcggacac cgtgcaactc ctgatgggtg tggtggtatt ctgcgcgtcg ctgctggcca
     1021 tgcggcgctc gcgaacactg ggccgcctgt tttggaaact caacgcgatt gcagttcttc
     1081 tgtggtgcgc cggacaggct ctgggcgcct actacggaag ctacctgaat ctgcccaccc
     1141 gcagtctctg gttcgtggac attttttatg tggcgtggcc ggcaccgctg gtgatgtgcc
     1201 tctttctgga cagcgaggaa gaaccggaag gcgcgaattg gcgcaggttg ctggactttg
     1261 gccaagtggc cattgtcttc gtgttgctgt tcttttattt cgccgcgttg tccgcgcaga
     1321 attccgcgtg gtccacctac gggctttccg ccgccaccga cggcttgctg accgccgcgt
     1381 ttctggcgcg agcattttcg atgcgcggtg agcaggtaag aaaactattt cttggaattg
     1441 ggcttttccg ggccgtggcg cttttgaccg atgtttattt cctggctggc ctccccgagt
     1501 tcgggaatgg cacgtggttt gacctggtct ggagtgcccc gtggctgatt ccgttggtca
     1561 cggccatatc ctggaccgac gcaaagcaag tacaaccagg agcgttcgag gcgagccgac
     1621 ggcggccaag aatgctactg gcacatatcc ttccgctgat ttttcctgtg ctggtggtgg
     1681 tgatggccgc gagcgttgcg ccgacgcagc tgaaaattgc ggcgctggcc gtgctgcttt
     1741 cgctgggcac gtcgtacgcg cgactcctgc tgacgcacga cgcgttgcga cgcagcaccg
     1801 aagcacttcg taatcaacac gaaatgttga acgcaatcgt tgaaggcacg accgcggcga
     1861 cgtacgtgaa ggatctgcaa ggccgctacc tccttatgaa ctccgcaggc gcaacgcttc
     1921 tgggcaaggg cgtgagcgaa attattggaa aggatgattt ggctttgttt tcaggtgaat
     1981 cggcgcggga gatcatcgct cgcgatcgcg agaccataga gtcaggcgtc atgcaaacct
     2041 atgaacagga catttccaga gacggcgtaa cgcggacgtt gcttgccacc aaagggccgc
     2101 tgcgagatgc gcagggccgc atcgccggag tggttggcag ttcgctggat attacggagc
     2161 gttaccgagc gatcgaggcg ttggccgaga gcgaagaacg tttccgaacg attttcgacg
     2221 ggtccccgat cggaatggcg gtgatcggca cggacggcag catcgtggcg tcgaattcgg
     2281 cttgccgtca gatgctgaac ctcaaagaag aagctctgag gggagttgcg atttttgatg
     2341 aactgacgca tcccgacgac cgggcggctg atgcggcacg atttcagcaa ttgattcgcg
     2401 gcgaaatcga atacgaccgg cgtgaaaagc gctacattct gcgggacgga cggcgagttt
     2461 tcgccgatct ccatctatat ctggtgcgcg acaaacgcgg agcggcgcgc tacttgattg
     2521 gcatctccgt ggacattacg gaacgtaaat cgctggaaac gcagctgcga caagcgcaac
     2581 ggatggaaac gatcggacgc ctggcgggcg gagtggcgca cgacttcaat aatttgttga
     2641 tggtgatcaa gggttattgc gaattg
//
LOCUS       DASTPM010000092         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_752909, whole genome shotgun sequence.
ACCESSION   DASTPM010000092 DASTPM010000000
VERSION     DASTPM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752909"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1856
                     /locus_tag="VFR42_01630"
     CDS             456..1856
                     /locus_tag="VFR42_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HEU0367878.1"
                     /translation="MPLMPDPTFYPSPRMAMQAKPERLAYVALLNVTKNGKRDAIGVV
                     DLEPGSKSFGRLVGQTDFPHNDNELHHFGWNACSACLCPQNPHPHMERRYLIVPGIGS
                     SRMHILDTKENPRQPKIVKVIEPEEFIRKTGYTSPHTVHCGPDGIYGSALGAANGDGP
                     GGIFLMDAETFELKGQWEQERGPQHLAYDFWWHLGHDTMVTSEWGTPNMVKDGVNPEL
                     LLGGKYGHKLHVWNLDKRKHLQEIELGAEQQMVLELRPSHDPNKAYGFVGVVLSLKDL
                     SSSIWMWYREGNGKWAVRKVIEIPAEPAQADDLPPLLKGFKAVPPLVTDINLSLDDKF
                     LYASCWGTGEFLQYDVSDPANPKKTGSICTGGIVRRAAHPQNPKRALNGGPQMVEISR
                     DGKRIYYTNSLYSPWDEQFYPDGVKSWMVKIDASPAGGMKLDENFYLEFDGLRAHQLR
                     LEGGDASSDSYCYSDK"
     gene            1874..2518
                     /locus_tag="VFR42_01635"
     CDS             1874..2518
                     /locus_tag="VFR42_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367879.1"
                     /translation="MSAAAFWLMLLLGAYHGLNPAMGWLFAVALGMQRQQGSAVARSL
                     VPIALGHALAVGSAVVIAAILGLSLPLVVMRSIVATLLVGLGIFFLVRHWHPRWIRMQ
                     VGFRDLTVWSFLMATAHGAGLMVVPVLLRGSTAQASGATAGRDLAHFTATTSAAAAIF
                     ATAIHTAGYLAVTGIIAWIVYRRVGLAVLRKAWFNVDLLWAVALVSTGVVTFLM"
     gene            2602..3015
                     /locus_tag="VFR42_01640"
     CDS             2602..3015
                     /locus_tag="VFR42_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0367880.1"
                     /translation="MSTFGLIEYKDASPEVRAVYDDIMATRKTDWINNFWKAIAHDPA
                     TLKRTWESIKQIMGPGALDPLTKEMIYVAVSVANNCHYCIASHTAGAVKKGMTPEMFK
                     ELQAVIGMAAETNKLVTGYRVEIDPQFELPFGAAK"
BASE COUNT          686 a          862 c          966 g          630 t
ORIGIN      
        1 gtatgtctaa gtggctgcgt ggaacgcgca aaacggcgcg acgacgcaag acaaagggtg
       61 cggcgagaaa gtgacggcaa gccgacatct ccatgaacat ctccgtgacg ctcgcggtta
      121 cgatttaatt caaacaagaa aaggcgactg acgctgcaat ctgcgcgcgt ttaacgacgt
      181 tgcgaggtag agcgaatcgc ccgcgagcga tcctctgatt tggaagggtc gtagtggtcc
      241 agagaagcca gaagcgttat cctcacagga actgagatcc gggcgtccag acttgtggtg
      301 taaaaagtgg atggcggttc ttggcagacg ttggcttcgt tgcgtcccca cggtcccgca
      361 cctatctcga ggctcttgcc tcgcgactgc ggaacacgtg ccccgcgacc tctggtgcag
      421 acccttcgcc tgatttttca aaaggagaat ctcgtatgcc tctgatgccg gacccaactt
      481 tctacccctc cccacgcatg gccatgcaag cgaagccgga acggctcgca tatgtagcct
      541 tgctcaacgt cacgaaaaac gggaagcgcg acgcgatagg cgtggtggac ctcgagcccg
      601 gctcgaagag tttcgggcgg ctcgtggggc aaacggattt tccgcacaat gataatgagc
      661 tgcaccactt cggctggaac gcctgcagcg cttgcctctg cccgcagaac ccccatccgc
      721 acatggaacg gcgctacctc atcgtacccg gcatcggctc ctcgcgaatg cacattctgg
      781 acaccaaaga aaatccgcgg caaccgaaaa ttgtgaaggt gatcgagccg gaggagttca
      841 ttaggaaaac cggctacacc tccccgcaca ccgttcattg cgggccggat ggaatttacg
      901 gaagcgcact gggcgcggcg aacggcgacg gccccggcgg tattttcctg atggatgcag
      961 agacgttcga actgaaaggg caatgggagc aggagcgcgg cccgcagcac ctggcctatg
     1021 acttctggtg gcacctcgga cacgacacca tggtgacctc ggaatggggc acgccgaaca
     1081 tggtgaagga cggcgtgaac ccggagctgc tgctcggcgg aaagtacggc cacaaactgc
     1141 acgtctggaa cctggacaaa cggaaacacc tgcaggaaat cgaactcggc gcggaacagc
     1201 agatggtgct cgaactgagg ccgtcgcacg atcctaacaa agcatatgga tttgtgggtg
     1261 tggtgctttc gctgaaggat ctgtcctctt cgatctggat gtggtatcgc gagggcaacg
     1321 gaaagtgggc agtgcgaaag gtaatcgaaa ttccggctga gcccgcccag gcggacgacc
     1381 tgccgccctt gctaaaagga ttcaaggcag tgccgccgtt ggtaaccgac atcaacttgt
     1441 cgttggacga caaattcttg tacgcgtcgt gctggggaac gggcgaattt ctgcagtacg
     1501 acgtttccga tccggccaat ccgaagaaga ccggatcgat ttgcaccggt ggaattgtgc
     1561 ggcgtgccgc gcatccacaa aacccgaaac gagccctgaa cggcgggcca cagatggtgg
     1621 agatcagccg cgacggcaag cgcatttact acaccaattc actgtattcg ccctgggacg
     1681 agcagtttta tcccgacggt gtgaaaagct ggatggtgaa aattgacgcg tctccggccg
     1741 gcgggatgaa gctggacgag aacttctacc tggaatttga cggactgcgc gcgcaccaac
     1801 tacggctgga aggcggtgac gcttcgtcgg attcctactg ctattcggac aaataataat
     1861 ggtgcattca gatgtgagcg cagctgcgtt ttggctgatg ctgctgctcg gcgcgtacca
     1921 cgggctgaat cccgcgatgg gatggctgtt cgcggtggcg ctgggcatgc agcggcagca
     1981 aggcagcgcc gttgcgcgct cgctggtccc cattgcactg gggcacgcac tggcggtggg
     2041 aagcgcagtg gtaatcgccg ccattttggg gttgtcgctg ccgcttgtgg tgatgcgaag
     2101 tatcgtcgct acgctattgg tgggcctggg cattttcttt ctcgtacgcc attggcatcc
     2161 gcgctggatt cgcatgcaag tgggcttccg cgatttgacc gtgtggtctt tcctgatggc
     2221 caccgcccat ggagccggat tgatggtggt gccggtactg ctgcgcggaa gcacggcaca
     2281 agcgtccggg gccacggctg gtcgcgatct tgcgcacttc acggcgacga ccagcgctgc
     2341 ggcggcaatc ttcgctaccg caatccatac cgctggatat ttggccgtga caggcataat
     2401 cgcctggatc gtatatcgca gagttgggtt ggcggtgctt cgtaaagcct ggttcaacgt
     2461 ggatctgctg tgggccgtgg cgcttgtttc gacgggcgtg gttacgttcc tgatgtaaga
     2521 tgcctgaatt cgattgcgcc gtgacgatgc gataggtgcc ggtgcaaccc ggtgcagcta
     2581 atatttttcc gagggataag catgtccaca tttggattga ttgagtacaa agatgcgagc
     2641 ccggaagttc gcgccgtgta tgacgacatc atggccacgc gcaagacaga ctggatcaac
     2701 aacttctgga aagccatcgc gcacgatccc gcaaccctga agagaacctg ggaaagcatc
     2761 aaacagatca tggggccggg ggcgctcgac ccgctaacga aggaaatgat ttacgtcgcg
     2821 gtaagcgttg cgaacaactg ccactactgc atcgcgtcgc acacggcggg cgccgtgaaa
     2881 aagggaatga ccccggagat gttcaaggaa ctgcaagcgg tgatcggtat ggccgcggag
     2941 acgaataaac tggtgaccgg ctaccgcgtg gaaattgatc cgcagtttga attgccgttt
     3001 ggagcagcca agtaacactg cccagcagcc actttgctgc gatcttctcg ctcgcatgct
     3061 tctcgacata acttctcgcg cagcgccgaa ttgaacgatg ccatccgtac ctgtacctgc
     3121 ggacaggtgt ttccacggta gtac
//
LOCUS       DASTPM010000093         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_810587, whole genome shotgun sequence.
ACCESSION   DASTPM010000093 DASTPM010000000
VERSION     DASTPM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810587"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="VFR42_01645"
     CDS             complement(<1..496)
                     /locus_tag="VFR42_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367881.1"
                     /translation="MNRLVIGLFNWILRFCIARFAFEPQDVLDIRNLAIAPNKQNNFL
                     TPVAAASYTQRPRNPGNYNAIHQPSFYTDEQMANKGHQWAATKQRIMEDTSFDATMDV
                     DAFLAGWALFFTLGVETVTGTGPFTHTMKFLQSTNQMPVTSLLCQETNDVIFQLPDMA
                     ILDLT"
     gene            complement(493..753)
                     /locus_tag="VFR42_01650"
     CDS             complement(493..753)
                     /locus_tag="VFR42_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367882.1"
                     /translation="MAKEDLVEVQLTEFGETVAAGSVVQVHEGNHSFYFTPGEAQLVT
                     RAFDWHKVLRNQHLNGHPLFEIVPDKVEPSTTEDTKDTEVSA"
     gene            complement(763..1248)
                     /locus_tag="VFR42_01655"
     CDS             complement(763..1248)
                     /locus_tag="VFR42_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367883.1"
                     /translation="MSLRKILDQTAVMAAAVANSGKVYKHLVWSTDDADLVSLFQDSQ
                     NRMNTIMITRESTASIDRGPNDNRDRHTIALKWYRSATRAADASTATEDTFQDDVEAM
                     RTAFKSNRKLTDGNGVRQAIYCDPMIARLVGFVMFRGVLCNYAELVFVAEDGPFNTTS
                     V"
     gene            complement(1248..1766)
                     /locus_tag="VFR42_01660"
     CDS             complement(1248..1766)
                     /locus_tag="VFR42_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367884.1"
                     /translation="MAGQNTITITINGLAEATGELQAAMMAAIPIGLEKVGLRGVPLV
                     QDHTPVGATGNLFGGVFAEFHQDGPLMEEIIGVHPPADVYAGPVELGTRPHFPPPSAL
                     LLWVQKKLNVTDEKQALSIAYAIAKTIAKRGTQGQHMFDQALAQLQLEAPGIMEATIA
                     EALTAAGFGGKD"
     gene            complement(1766..2509)
                     /locus_tag="VFR42_01665"
     CDS             complement(1766..2509)
                     /locus_tag="VFR42_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367885.1"
                     /translation="MIFSDFETSVTSRIQATGSKINQADQDDAITQAVKRYSKDRPRE
                     LVTDITGVNPASAILALPTGPGNPPEQFEDGFSIIRAIEFPVGNLPPTYLEGEDWLLY
                     RTPAGLKLALTSLVPQTTDTLRVTWTLRHNPGTSGQNAIATTVPDSDFEAVCDLAASF
                     CCDKLSAAYARTSDSTISADAVNYRTKAQEYAAIAKQYKKRYDDHIGIEDSGTGAAGA
                     NPAAGAISIGNMFETQGSGVDRLTHRRPR"
     gene            complement(2513..2767)
                     /locus_tag="VFR42_01670"
     CDS             complement(2513..2767)
                     /locus_tag="VFR42_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367886.1"
                     /translation="MVTRLVCEASYRLARAGGAAMTQEEDSLVDEIMTTEFRLRENQR
                     VQRRVNSVRKGGRGESPEPLPVRSRRFANASTRLCLVMKK"
     gene            complement(2785..>2980)
                     /locus_tag="VFR42_01675"
     CDS             complement(2785..>2980)
                     /locus_tag="VFR42_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367887.1"
                     /translation="DDQGSVQDQRLNKIWHAAHLLVARQVLVKAARKQGASRPSFKEL
                     LWMRGSGNSQTVRSLSCWAA"
BASE COUNT          540 a          904 c          878 g          658 t
ORIGIN      
        1 tggtcaggtc caggatcgcc atgtcaggca gctggaagat gacgtcgttc gtttcctggc
       61 acagcaggga tgtgaccggc atctggttcg tcgactggag gaacttcatg gtgtgcgtaa
      121 agggcccggt ccccgtaacc gtttccacgc ccagcgtgaa gaacagcgcc cagccggcca
      181 ggaacgcgtc gacgtccatg gtggcatcga acgatgtgtc ttccatgatg cgctgcttcg
      241 tggccgccca ctgatggcct ttgttggcca tttgctcgtc tgtatagaac gacggctggt
      301 gaatcgcgtt gtagttgccc ggattgcgcg ggcgctgcgt gtagctggcc gcagccactg
      361 gcgtgaggaa gttgttctgc ttattcggcg cgatcgccag gttgcggatg tcgagcacgt
      421 cctgcggctc gaacgcgaac ctcgcaatgc agaaacgaag aatccagtta aagagtccga
      481 tgactaagcg attcaagcgc tcacctccgt gtccttggtg tcctctgtgg ttgagggttc
      541 taccttgtcc gggacgatct caaacagcgg atgcccgttg aggtgctggt tcctcagcac
      601 cttgtgccag tcgaatgcgc gcgtgaccag ctgcgcttcg cccggcgtga aataaaacga
      661 gtggttaccc tcatgcacct gcaccacgct gccggcagcg actgtctcgc cgaattccgt
      721 gagctgcact tccaccagat cttcttttgc catcactacc tcctagaccg atgtcgtatt
      781 gaacggcccg tcttcggcca caaaaaccag ctccgcgtaa ttgcagagca cacccctgaa
      841 catcacaaag cccaccagcc gcgcgatcat tggatcgcag taaatggcct ggcgcacgcc
      901 gttcccatcc gtcaacttgc ggttgctctt gaacgccgtc cgcatcgctt cgacgtcatc
      961 ctgaaacgtg tcttctgtcg cggtggacgc gtctgcagcg cgcgtggcgc tgcggtacca
     1021 cttgagcgcg atcgtatgcc ggtcgcgatt gtcattcgga ccgcgatcaa tcgaagccgt
     1081 actctcgcgc gtgatcatga tcgtgttcat ccgattctgg ctgtcctgaa aaagcgaaac
     1141 caggtccgcg tcgtcggtgc tccagaccag gtgtttgtaa accttgccgc tgttcgctac
     1201 ggccgcagcc atgacggcag tctggtcgag aattttacgg agagacatca gtccttacct
     1261 ccgaagccgg ccgctgtgag cgcctccgcg attgtggctt ccatgatgcc cggcgcttcc
     1321 agctgcagct gcgcgagcgc ctggtcaaac atgtgctgcc cttgcgtgcc gcgtttcgcg
     1381 atggtcttcg cgatggcata cgcgatcgag agcgcctgct tttcgtcggt gacatttaac
     1441 ttcttctgca cccacaagag cagagcggaa ggcggcggga agtgcggccg cgtgcccagc
     1501 tcgacaggac cggcgtaaac atcggccgga ggatgcacgc cgatgatttc ttccatcagc
     1561 ggaccgtcct ggtgaaactc cgcgaagacg ccaccaaaga gattgccagt cgctcccact
     1621 ggcgtgtggt cctggaccag aggaacgccg cgcaaaccga ccttctccag cccgatcgga
     1681 atcgcggcca tcatcgcggc ctgcaactcg ccggtggctt ccgccaggcc gttgatcgtg
     1741 atggtgattg tgttctgtcc agccatcagc gcggtctcct gtgggtgagc cggtccacac
     1801 ccgagccctg cgtctcaaac atgttgccga tggagattgc gccggccgca ggatttgcgc
     1861 cggcagctcc cgtgccgctg tcttcgatcc cgatgtgatc gtcatagcgc tttttgtatt
     1921 gcttggcgat cgcggcgtac tcctgcgcct tggtgcggta attcaccgcg tcagcgctga
     1981 tggtggagtc gctggtccgc gcatacgcgg ccgagagctt gtcgcagcag aatgacgcag
     2041 ccaggtcgca cacagcttca aagtccgagt cgggaacggt tgttgcgatc gcgttctggc
     2101 cggaagtgcc cggattgtga cgcagcgtcc aggttacgcg cagcgtgtcc gtggtctgcg
     2161 ggactaagct cgtgagagcc agcttgagtc ctgccggcgt gcgatagagc agccagtctt
     2221 caccttccag atatgtaggc ggcagattgc ccacgggaaa ctcaatggcg cgaatgatgg
     2281 agaaaccatc ttcaaattgt tcaggagggt tgcccggtcc tgtgggcaac gccaggatcg
     2341 cgctggccgg attcacgccg gtgatgtctg tcaccagttc gcgcggccgg tctttggaat
     2401 agcgcttcac ggcctgcgtg atggcgtcat cctggtccgc ctggtttatc ttgctgccag
     2461 tggcctgaat gcgcgatgta actgaggttt cgaagtcgga aaagatcata ggctactttt
     2521 tcatcaccag gcacagccgc gtgctggcgt tagcaaaacg acggctgcgc actggcaatg
     2581 gctctggcga ttctccgcga cctcctttcc tcaccgaatt aactcgccgc tgcacgcgct
     2641 ggttttcgcg caagcgaaac tccgtggtca tgatctcgtc aacgagactg tcttcttcct
     2701 gggtcatggc tgcgccgccc gcgcgcgcca ggcgatagct tgcttcgcag accagccgag
     2761 tcaccatgtt gatctcgcct gtattcaggc tgcccaacat gagagcgatc tgacggtctg
     2821 agaatttccc gagcctcgca tccatagcaa ctctttaaaa gaaggccgcg acgccccctg
     2881 tttgcgcgcg gccttcacca gcacctggcg tgcgacgagg agatgcgccg catgccagat
     2941 tttgtttaaa cgctggtctt gaactgaccc ctgatcgtca
//
LOCUS       DASTPM010000094         2226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_579868, whole genome shotgun sequence.
ACCESSION   DASTPM010000094 DASTPM010000000
VERSION     DASTPM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579868"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFR42_01680"
     CDS             <1..483
                     /locus_tag="VFR42_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0367888.1"
                     /translation="DYPLAEFDNVIAVHLRAAFLLSKLVLPEMYSRRSGVILNISSLS
                     AKSAFAWGSAYAAAKAGMLGLTRVAAAEAARKGVRINALCPGPVTETVMSQKLGATLA
                     KKMGVSPKEQLEGFLSTLLQGRAQTAEEIAQAALFLCSDQSSAITGQSVNVDGGAAFF
                     "
     gene            complement(526..>2226)
                     /locus_tag="VFR42_01685"
     CDS             complement(526..>2226)
                     /locus_tag="VFR42_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0367889.1"
                     /translation="RYRQQFANFADAGSDHVPKVESLADSLVGESRSSLLVLLAAVGF
                     VLLIACANVANLLLARATAREKEMALRKALGASRWRLIAQLLSESFVLSFIGSALGVG
                     LAFALMPLLRSLKQASLPRLAETRVDAAVLLFSLALCIVTAALFGLAPALQAAGKQLH
                     ESLKEGIRGSSAGGRRGRLRGVLVIAEMAVALILITAAGLLAESFARLLQVNLGFSPQ
                     NVTTFPVVLPSTRYAKPEQLTEFYRVALQRIRALPGVETAAFVSFLPLSGGYRLSYFC
                     AEGQVCQGLGKDPLIAFWQVTPGYFETMRAPLLRGRFFDEHDIAGAAPVIIVNETAAR
                     HFWPNENPLGKHVAGSRDLIPRQVVGVVADAKFSALKAVNAEQFYVPSEQMPYPAMTL
                     VVRSTADRESLVTAIRAKLAEVDPTLPISGILSMEAVIASSIAQPRLITQLVGAFAIF
                     ALLLAAIGMYGVMAYSVSQRKQELGIRMSLGAAPGDILRLVVRQGMTLAAIGTLVGIT
                     GSLALTRLMTSLLFRVQATDPLIFGSAALVLAVASFAACYIPARRATRVDSIIVLRHE
                     "
BASE COUNT          494 a          668 c          677 g          387 t
ORIGIN      
        1 gattatccgc tcgcggaatt cgacaacgtc attgcagttc acctgcgtgc cgcgtttcta
       61 ctgagcaagc tggtccttcc cgaaatgtac tcgcggcgtt ccggagtgat tctgaatatt
      121 tccagtttgt cagcgaagtc ggcatttgcg tggggttccg catatgctgc cgccaaggcc
      181 gggatgttgg ggctgactcg cgttgccgcc gcggaagcgg cgcgcaaggg cgtgcggatt
      241 aacgcgctgt gccctggccc ggtgacggaa accgtgatgt cgcaaaagct tggcgccacc
      301 ctggccaaaa aaatgggcgt cagcccgaag gaacagctgg aaggatttct cagcacgctg
      361 ttacaagggc gcgcgcaaac cgcggaggaa atcgcgcagg ccgcgctgtt tttgtgctcc
      421 gaccaatcca gcgccatcac tggacaatcg gttaacgtgg acggtggcgc ggcctttttt
      481 tgaaacgatt ccgttttgcc cacatgaaac gcggcgccga tttggctatt cgtgacgcag
      541 gacgatgatg gagtcgaccc gcgtagcgcg tcgcgctgga atgtagcagg ccgcgaagga
      601 agcgacggcc agcaccagcg cggccgagcc aaaaataagc ggatcggtcg cttgcacccg
      661 gaaaagcagg ctggtcatga ggcgagtcag ggccagcgaa ccggtaattc ccaccaacgt
      721 cccgatagcc gccagcgtca tgccttggcg caccaccaac cgcaaaatgt caccgggcgc
      781 cgctccgagc gacatgcgaa tgcccagttc ctgcttgcgc tgggacaccg agtaggccat
      841 gacgccatac atcccaatgg cggcgagcag gagcgcaaaa atggcgaagg cgccaaccaa
      901 ttgcgtgatc aagcgcggct gcgcaatgga gctggcgata accgcctcca tgctgaggat
      961 tccggagatg gggagagtcg ggtcgacctc cgccagcttg gcacgaatcg cggtcaccag
     1021 cgattcgcga tccgcggtcg aacggacgac cagcgtcatc gcgggatatg gcatttgctc
     1081 cgaaggaacg tagaactgct ccgcgttcac tgctttcagg gcactgaatt tcgcgtcagc
     1141 tactacgccg acgacttgcc gcggaatcaa atctcgagaa ccggcgacgt gttttcccag
     1201 tggattttca ttgggccaga aatgcctggc ggcagtctcg ttgacgatga tgaccggagc
     1261 cgcaccggcg atatcgtgtt catcgaaaaa ccggccgcgt aatagtggcg ccctcatagt
     1321 ctcgaagtag ccgggcgtca cctgccaaaa cgcgatcaag ggatccttgc ctaggccctg
     1381 gcagacttgc ccttccgcgc agaagtaact gagccggtag ccgcccgaga gcggaagaaa
     1441 gctcacgaac gcagcggtct cgacgcccgg cagggcgcgg atccgctgca aggccacccg
     1501 gtaaaactcc gtgagttgtt ccggcttggc atagcgcgtg gagggaagaa caaccgggaa
     1561 cgtagtgacg ttttgcggcg aaaatcccag gttgacctgc agcaggcgcg caaaactttc
     1621 ggccagcaag ccggcagcgg tgatgagaat aagcgctacc gccatttccg caatcaccaa
     1681 aacgccgcgc aatcttccgc ggcggccgcc tgcggaggag ccgcgaatgc cttccttaag
     1741 cgattcgtgc agctgcttcc cggcagcctg tagcgcaggc gccaggccga agagcgctgc
     1801 ggtgacaatg catagcgcca gggaaaacag cagcaccgca gcatcgacac gcgtttccgc
     1861 aagccgcgga aggctggctt gcttcagcga acgtagtaga ggcatcagcg caaaagccaa
     1921 gcccaccccc aaagcgctgc cgatgaagga caaaacgaag ctttcgctga gcagttgggc
     1981 aatcaatcgc catcgtgacg cgcccagcgc tttgcgcaaa gccatctctt tctcgcgcgc
     2041 ggtggcgcgc gccagcaata gattggccac attggcgcaa gcaatcagca agacaaatcc
     2101 aacggctgcg agcaatacca gcaacgacga tcgcgattct cccaccaggc tgtccgccag
     2161 ggattccact ttaggcacgt gatcactgcc ggcatccgcg aagtttgcga attgttggcg
     2221 atagcg
//
LOCUS       DASTPM010000095         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_61004, whole genome shotgun sequence.
ACCESSION   DASTPM010000095 DASTPM010000000
VERSION     DASTPM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61004"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /gene="groEL"
                     /locus_tag="VFR42_01690"
     CDS             <1..1134
                     /gene="groEL"
                     /locus_tag="VFR42_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU0367890.1"
                     /translation="GVITVEEAKSLETELEVVEGMQFDRGYISPYFVTNAEKMRVEME
                     DPYVLIYEKKLSGLQELLPLLEAVVQTSKPLLIIAEDIEGEALATLVVNKLRGGLKVA
                     AVKAPGFGDRRKAMLEDIAILTGGTMVSADLGIKLENVTINMLGRAKKVMIDKENTTI
                     VGGAGKKADIQARITQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEIEVK
                     ERKDRVDDAMHATRAAVEEGVLPGGGVALLRASETLKKVRTHNDDQKTGVEIVRKALS
                     WPARQIAINAGEDGSIVVGKILEKDTYAYGYDAQAGEYGNLISKGIIDPTKVVRAALQ
                     GAASVAGLLITTEAMVAELPKKQSPAMPGGPGGGMGGMGGMDF"
     gene            1306..1482
                     /locus_tag="VFR42_01695"
     CDS             1306..1482
                     /locus_tag="VFR42_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367891.1"
                     /translation="MRTHRFGQKIKLPATIAIKHQLRKLMFEDGVKKAEAGARAEAIR
                     GQLREETQPLVQSG"
     gene            2636..2800
                     /locus_tag="VFR42_01700"
     CDS             2636..2800
                     /locus_tag="VFR42_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367892.1"
                     /translation="MEEYQPSFDQLRNNAGECIRLAEAARTLAHKSFFIEMADRWLTL
                     AERAQKTQER"
BASE COUNT          709 a          895 c          963 g          563 t
ORIGIN      
        1 ggcgtgatca cggtcgagga agcaaagtcg ctcgagaccg agctcgaagt ggtcgagggc
       61 atgcagttcg accgcggcta catctcgccc tacttcgtga ccaacgctga gaagatgcgg
      121 gtcgagatgg aggatcctta tgtcctgatc tatgaaaaga agctgtccgg actgcaggag
      181 ttgctgccgc tgctggaagc ggtggtgcaa acgagcaagc cgttgctgat cattgccgag
      241 gacatagagg gtgaggcgtt agccaccctc gtggtcaaca agctgcgcgg cggcctcaag
      301 gtcgcggcgg tgaaggcccc cggcttcggc gatcgccgca aggcaatgct cgaggacatt
      361 gcgatcctca cgggcggcac catggtttcc gcggacctcg gcattaagct cgagaatgtg
      421 acaatcaata tgcttgggcg tgccaagaaa gtgatgatcg acaaggagaa caccacgatc
      481 gtcggtggcg ctggcaagaa ggccgacatc caggcgcgta ttacccagat caaggcgcag
      541 atcgaggaaa cgacctccga ctacgaccgg gagaagctgc aagagcgact ggccaagctc
      601 gccggcggcg tcgcggtgat ccgcgtcggc ggcgcgaccg agatcgaggt gaaggagcgc
      661 aaggaccgcg tagatgacgc catgcacgca acccgcgccg cggtcgagga aggtgtcctg
      721 ccaggcggcg gcgtggcgct gctgcgcgca agcgagacgc tcaagaaggt ccgcacccac
      781 aacgacgacc agaagaccgg cgtcgagatc gtgcgcaagg cgctctcctg gcccgcgcgt
      841 cagatcgcca tcaatgccgg cgaagacggt tcgatcgtgg tcggcaagat cttggagaag
      901 gacacctatg cctacggcta tgacgcgcag gcgggggagt acggcaatct gatctccaag
      961 gggatcatcg acccgaccaa agtggtgcgc gcggcactgc aaggtgcggc atcagtcgcc
     1021 ggcttgctca tcacgaccga ggccatggtc gccgagctgc cgaagaagca gagcccagcg
     1081 atgccgggcg gtcccggtgg aggcatgggc ggaatgggcg gcatggactt ctgaggcgct
     1141 cgatcgatca tcgaaaataa agaaggcctg agcacgctcc ggccttcttt gaaatctgac
     1201 acttgttcct gcggctcaac tggcaacccg caagtgcggg ggcgcagcgg tcgcaattgg
     1261 ccaggacgtg ccaggcgtaa gagtactcgg ccacaggagc ggccaatgcg cacgcaccgg
     1321 ttcggacaaa aaatcaaact tcctgcgacg atcgccatca aacatcagct cagaaagctg
     1381 atgtttgaag atggagtcaa gaaggcagag gcaggagcaa gagctgaagc aattcgcgga
     1441 caactgcgcg aagaaacaca accgctcgtg cagtcgggct gagacgtagt tgcagggcga
     1501 ccagctcttt cgcgagtgcc tggaccagat tcggggctat gacgcaaatt tcggtgcgtt
     1561 gatcgtcgag cgctacccct ttcgcgtcat ccggttggcg tactcttttt gacagtgatg
     1621 acaggacact atggtctggc gggccacgaa tccatcctag tcgtatcggg gggctattcg
     1681 accaggatct taaaaattca acctgagaca ccccccagag cgcacctctt caaatactcc
     1741 gggcggtact ggccgggttt aagccctcgt ggccgatttt gaatttcaca gatttccaag
     1801 taacgtgcct caacggggtc ggggggtgcg aagttcaagg aggtccgaac ccgatgcatc
     1861 cgccggaaga atttctaaaa catgctgcgg agtgtcagct aacggccaaa cttgcgcgcg
     1921 acccggcgag caaggctgcg tggagccaga tggcggagag atggcttcaa tgtgctgaat
     1981 tggcgcaacg caaaagcctc acggcacgct acgccacccc cgtggggcga tcccgcagac
     2041 cctcccctgc ttggcgtcat ctttgagttc tagcgagata gaggccgcct tggttggcgg
     2101 cccgcgtcat atggggacaa tcgcaccaat tagcgacatc agcggttgaa acatccgcgc
     2161 gccctcggga cggacccgcg cctcctccgt cgagatggcc aagcggctcg actgaacttg
     2221 aagcgcttcc gacgacctgc cgccaggcca attggcgaac tgcgctacgc caagaaggtg
     2281 cttagcgttt aattttcgac cagccattgc gctttgcgcc ctgtacccca tgtgatctga
     2341 tacgagcaag caccggcttg tccgagagct gcccaaaaaa cgcagacgta ccgaagtctc
     2401 cgtttccctg cagtggcagc cctggtgccg tcggcaggca caataacact gcaaatagag
     2461 ttgcgttcgg atgcggatgc cctaagcacc ccgcatctgg tgcgtgtccg cgcgctgcgc
     2521 ggcgcgcgtc ccccgcgcat cgaaagggca acacaggata cccggggcga ccacgcgagg
     2581 ccccccattc tcgagttcca aattctgaca tcgccaaagt agtacagaat tagagatgga
     2641 agagtaccag cccagcttcg atcagttacg caacaacgct ggcgaatgca ttcgattggc
     2701 ggaggcggcc cgcactttgg cacataaatc gtttttcatc gaaatggcgg accggtggct
     2761 tacgctcgcc gagcgcgccc agaagacgca agagagatag ctacacgagt ccgggcccat
     2821 tggagggttg gacagcggag accgagatgg tcatctatcc tttagaattt tatcgacgct
     2881 ccgaacggcg gtggaagcat cgtgtcaaag cctctacgcg atcccagcac gatgatacgc
     2941 ctacagaccg cggcaacgaa ctttgtccaa actgctgcct cccggtcgca agaccatttg
     3001 tgtctaggtt ccttgccgga tccgtcgttg aacatcattg gctgtgcaag acttgtgagt
     3061 ttagttggac cagtcgtttt catccgatgt tggtttaaaa taaagcgagc gaacgaccgt
     3121 gagagcaatg
//
LOCUS       DASTPM010000096         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_61026, whole genome shotgun sequence.
ACCESSION   DASTPM010000096 DASTPM010000000
VERSION     DASTPM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61026"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..923
                     /locus_tag="VFR42_01705"
     CDS             255..923
                     /locus_tag="VFR42_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0367893.1"
                     /translation="MEHPCHKCGQMVEDGVPFCKHCGAPQIRVVGFESATVPPLDSAE
                     APRISTMPPPPAFLPRAAGAPVVWSRALPGAALGGAFSLLALVIPFAIFGPAFLVGGA
                     IAVMLYRRQTAGALPTTAAGAQIGAASGGFGFLYSAVVAFATLVYSPTELRQAMLDSI
                     SQLAGRGYDPQKIAQVQELLKSPQGLTFFVVFALFLMFLITIVGSSIGGALYAAWLRK
                     RNLL"
     gene            1412..>2114
                     /locus_tag="VFR42_01710"
     CDS             1412..>2114
                     /locus_tag="VFR42_01710"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU0367894.1"
                     /translation="MNKVARGSLLGIEALDAGEILKILQLARRMNPQKPRPLLRGKRV
                     VLLFYEPSTRTRSSFEIAAKLMGAMTTLVQSSGSSIEKGESLLDTGYTLRAVGADVIV
                     IRHPLAGAPQLMAEHLDIPVVNAGDGMHEHPSQALLDAYTILKHKKTLKGLRVAIVGD
                     VFHSRVARSACYLLTKFGSKVTLCGPPELVPEVAATLAPGVSVTRHIEEALHGTDVIT
                     LLRVQKERLAGLKLRL"
BASE COUNT          368 a          604 c          689 g          453 t
ORIGIN      
        1 cgctgaagcg actggacctg tggtcgcgag tgggaatgac gcaccgctgg ttgtttccgg
       61 cgcccccggc gggctaacgc tttctagcag aaaccacggc ttaccccgtg ggttttgtct
      121 ggcgccggtt cgcggcttcg cgggcggggg cgcccgcgct acacagatgc gatcgatggg
      181 ttatgctcgc tgtcgatcgg ctcttgtctg cgtttcttgc ctgaatcctc cgggctacgt
      241 gaccttgaac ttcgatggag catccctgtc acaagtgcgg acaaatggtg gaggatggcg
      301 ttcctttctg caagcattgt ggagcgccgc agatccgcgt agtgggtttc gagtctgcca
      361 cggttccgcc actggattca gccgaggcgc cgcgcatttc gacgatgcct ccgccgcccg
      421 cattcctgcc tcgcgcggcc ggcgcaccag tcgtgtggtc gcgcgccctg cccggggccg
      481 ccctgggcgg cgcgttttca cttctggcct tggtcatccc ttttgccatc tttggcccgg
      541 cattcctggt gggaggtgca atcgcggtca tgctgtaccg gcgtcagact gcaggcgcac
      601 tgccgacgac cgcggccggc gcgcaaattg gggcagccag cggcggcttc ggctttctgt
      661 attcggcagt cgtggcattc gccactctcg tatacagtcc caccgagttg cgccaggcaa
      721 tgcttgacag tatttcgcaa ttggctggcc ggggctacga tccccagaaa attgcgcagg
      781 tgcaggaact gctcaagtca ccgcaagggc tgacgttctt tgtagtgttc gcactgttcc
      841 tgatgtttct gatcaccatc gtgggatcga gtatcggcgg cgcgctgtac gcagcctggc
      901 ttcggaagcg gaacttgctg tagatattcg gggagaattc gcgactgcgg gcctcccgtt
      961 tagagattca gagcagcgat gggtatcgag cgatggcggc tgtcaccgac caggagcgtg
     1021 cgagagttgg gttgtgcaga aattgcaagc atgcgcaccg tgtagagtcg gaccgcggat
     1081 caatcttttg gcgctgtgaa ttgtcggcgt ctgatcccag tttccccaag tatccccgcc
     1141 taccggttct ttcctgcagg ggatatgaac cgttgaacgg cccggaatta ctgccttcct
     1201 agcccggcat ggagaatgga gatatatggg cagctgcgta aaacgcgcgc gtgtaatgtg
     1261 cgtttccgcc atgcttgccg catggcgcag cgacgcgtag aatgctgcgt tcatccttcg
     1321 ccatcacgcg caaggcgagc cggacacggg gggaaggttg agcggaatcg gaagcgcagc
     1381 gctgcaacgc gcctgcctcc cgtgacaacc gatgaataaa gtggcacgcg gttctttgct
     1441 cggcattgaa gctctggatg ccggggaaat cctgaagatc ctgcaactgg cgcggcggat
     1501 gaatccgcag aagccgcgtc ccctgcttcg gggaaagcgc gtggtgctgc tgttctacga
     1561 accctccaca cggacgcgct cgtcgttcga aattgccgcc aagttgatgg gcgctatgac
     1621 gacgctggtg caatcgagcg gctcgagcat cgagaagggc gaatcgctgc tcgacaccgg
     1681 gtatacattg cgcgcggttg gtgcagatgt gatcgtgatc cgccatccgc tggcgggcgc
     1741 gccgcagttg atggcggagc atctcgacat tccggtggtg aatgcgggcg acgggatgca
     1801 tgagcatcca tcgcaagccc tgctcgacgc ttacacgatt ctgaagcaca agaagacact
     1861 caaggggttg cgggtcgcaa tcgtgggtga tgtttttcat agccgggtgg cccgatcggc
     1921 ttgctatctg ctcacgaagt ttggatcgaa ggtcacgctg tgtgggcccc cggaactggt
     1981 gcccgaagtg gccgcgacgc tggcacccgg ggtgagcgtc actcgtcata tagaggaagc
     2041 gttgcacggc acggatgtga tcacgctgtt gcgcgtgcaa aaagagcgcc tggctggact
     2101 gaaactgcgg ctcg
//
LOCUS       DASTPM010000097         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_983610, whole genome shotgun sequence.
ACCESSION   DASTPM010000097 DASTPM010000000
VERSION     DASTPM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983610"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFR42_01715"
     CDS             <1..1034
                     /locus_tag="VFR42_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEU0367895.1"
                     /translation="ESVRDLEKAQNDATAPVTVTRVLSYAKATTMTATLKKFLSSKGD
                     ILADDRSNQLIIRDIPSTIPIVDNLIHQLDRKSQQVEIEARVVAASRSFARDIGTELG
                     FAGTTTGGRSLFSGDPRVGGSGVTTGTGIPAPPVTIGAGGTSGNGVSTGLPLNTNFAA
                     GAPPTSGFGFSHRSPNFAIDFFISAAESKGVGKLLSKPRVVTQNNEKATVKQGTKIPI
                     QTTVNNTVSVQYIDAVLKLEVTPLITAEGTVFMDVLVENTQIDNGIARVQGIPALDTQ
                     SAETKVLVADGGTVVIGGIIVTQQQVTITQTPVVGSLPLIGNLFKRTQTTVTSQELLF
                     FLTPRIIPS"
     gene            1106..>2092
                     /locus_tag="VFR42_01720"
     CDS             1106..>2092
                     /locus_tag="VFR42_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU0367896.1"
                     /translation="MDTAYKRRIARLQTCIRKQRLDSLLVTSPYDWCYLTGFTGEAGV
                     LLVEPRGLTLVTDGRFTVQAKDEAPGLKVELQREGLYRTCGELLKRRQRRRAGFDAGQ
                     LTVEQMESLKKATGRTVRLEDAGGMVLSLRTVKDATELAAMRKAAALADQVVQSAVRL
                     LKPGVRELEIAAEIEYHMRKGGASGPAFESIVAFGERAALPHARPTAKRLKKGQLVVL
                     DLGAILNRYCSDITRTVFVGKAPAKVRLWYQAVLEAQAAAIAAVAAGRSCADVDAAAR
                     GVLAAYQLDQYFVHSTGHGLGLEVHENPRVAKGQKTLLASGNVVTIEPGVYVP"
BASE COUNT          437 a          598 c          652 g          405 t
ORIGIN      
        1 ccgagtcggt tcgtgatctg gaaaaggcgc aaaacgatgc tacggcgccg gtaaccgtca
       61 cacgtgtgct gagctacgcc aaagccacga ccatgaccgc gacactgaag aagtttcttt
      121 cgtccaaggg agacatcctg gccgatgacc gctccaatca gcttatcatt cgcgatattc
      181 cctccaccat tccgatcgtg gacaacctga tccaccagct ggaccgcaag tcgcagcagg
      241 tggaaatcga ggcgcgcgtc gtggctgcgt cccgttcctt cgctcgcgac attggtacag
      301 aactcggttt tgccggcact acgaccggcg gccggtcgct gttttctggc gatccccgtg
      361 tcggcggtag cggcgtaacc accggcaccg gcattcccgc gcctccggtc acgattgggg
      421 ctggcggcac gtcgggtaac ggcgtctcga ctggcctgcc cttaaacacc aacttcgccg
      481 cgggtgctcc tccgacttcg ggttttggtt tctcgcatcg ttcgccgaac tttgccatcg
      541 acttctttat ttcggcagca gagtccaagg gcgttggaaa gctgctctcc aaaccccgcg
      601 ttgtgaccca gaacaacgaa aaggcgacgg tcaaacaagg taccaagatt ccgattcaga
      661 ccactgtcaa caacaccgtt tcggtgcagt acattgacgc cgtgctgaag ctggaagtca
      721 ctccgctgat caccgccgaa ggcacggtct ttatggacgt gctggtggaa aatacccaga
      781 tcgacaacgg tattgctcgc gtgcagggca tcccggcctt ggatacgcag tctgccgaaa
      841 ccaaggtcct ggttgccgac ggcggcaccg tggtcatcgg cggcattatc gtgacccagc
      901 agcaggtgac catcacccag actccggtgg ttggcagctt gccgttgatt ggcaacctct
      961 tcaagcggac gcagacgacc gtcacgtcgc aggaactgct gttcttcctg acgccgcgca
     1021 tcatccccag ttgaagcagc ggctgaggac aaactctttc aactttgcag gaaatcgagg
     1081 agactaacat ctcctcgatt tttttatgga caccgcctac aagcgccgca tcgcccgttt
     1141 gcagacctgc atccggaaac aaaggctcga ctcattgctg gtgaccagcc cgtacgactg
     1201 gtgctacctg accggtttta cgggcgaagc aggcgtgctg ctggttgagc cgcggggcct
     1261 gacgctggta accgacgggc ggttcaccgt gcaggcgaaa gacgaagcgc caggtctaaa
     1321 ggttgagctg caacgcgaag gcttgtaccg cacatgcggg gaactgctga aacgccgcca
     1381 acggcgtcgc gccggattcg atgcggggca actgaccgta gagcagatgg agtcgcttaa
     1441 aaaagctacc ggcagaactg tgcggctcga ggatgcgggc gggatggtgc tgtcgctccg
     1501 aaccgtgaaa gatgcgacag agctggccgc aatgcgcaag gcggcggctc tggccgatca
     1561 ggtagtgcaa tccgcggtgc gcttactgaa gccgggagtg agggaactgg agattgccgc
     1621 cgagatcgaa taccacatgc ggaagggcgg cgcgtcaggg ccggcatttg aaagcattgt
     1681 ggcgtttggc gagcgggcgg cgctgccaca cgcacgacct accgctaaac gcttgaagaa
     1741 agggcagttg gtggtgctag acctgggtgc tatactcaat agatactgca gcgacattac
     1801 gcgaacagtt tttgtgggta aagcgcccgc gaaggttcgg ttgtggtatc aggcggtcct
     1861 ggaagcgcag gcggccgcca tcgctgcggt ggcagctgga cggagctgcg ccgacgtgga
     1921 cgcggcagcc agaggggttc tggcggcata ccagctggat cagtatttcg tgcacagtac
     1981 ggggcatggg ctgggcttgg aggtccatga gaatccgcgc gtggccaagg ggcaaaaaac
     2041 actgcttgcc tctggaaatg tggttacgat agaaccgggt gtgtacgtac cg
//
LOCUS       DASTPM010000098         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1416243, whole genome shotgun sequence.
ACCESSION   DASTPM010000098 DASTPM010000000
VERSION     DASTPM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416243"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /gene="rplB"
                     /locus_tag="VFR42_01725"
     CDS             complement(<1..305)
                     /gene="rplB"
                     /locus_tag="VFR42_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU0367897.1"
                     /translation="MTVLDKNEITKETPEKRLLEPKKRSGGRNNHGEVVVWHRGGGHK
                     KQYRKVDFRRDKDGVPAKVEAIEYDPNRSANIALLHYADGDKRYILHPVGLEVGATVT
                     "
     gene            complement(456..752)
                     /locus_tag="VFR42_01730"
     CDS             complement(456..752)
                     /locus_tag="VFR42_01730"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU0367898.1"
                     /translation="MSTPHYQIIRRPIITEKGLGVKEVQGTVVFEVSAKATKTQIKEA
                     VQQIFKVKVAGVRTANFVGKMRRRGQNEGYRRDWKKAYVKLAAGEKMIEFAENL"
     gene            complement(756..1520)
                     /gene="rplD"
                     /locus_tag="VFR42_01735"
     CDS             complement(756..1520)
                     /gene="rplD"
                     /locus_tag="VFR42_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU0367899.1"
                     /translation="MPVVDVVNLDGKKVGEVELADAVFGAKVNAHLLHEASRWYLAGL
                     RRGTHKVKEKSEVSGAGRKLWRQKGTGRARVGSIRSPLWRHGGTVHGPRPRDYSYALP
                     KKQLLGALRSALSAKLADQKLTVVDAWQLESHKTQSLLASLEKLNFTKSALLVSHGEN
                     RNLELASRNLEGVKLSAPNALQPYEVLKHDRLVLSKDAVARLVRSLDPEKKPVVVPDV
                     ETIAAAPVKKEAAARKPAAKKPAKPRGKSAAKKGKE"
     gene            complement(1587..2228)
                     /gene="rplC"
                     /locus_tag="VFR42_01740"
     CDS             complement(1587..2228)
                     /gene="rplC"
                     /locus_tag="VFR42_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU0367900.1"
                     /translation="MAVNGIIGIKLGMTQIFAEDGNLVGCTVLQAGPCVVVQRRTKEK
                     DGYDAAQLGLVEFVKPQRVNKAMTGHFKKADVAPMKVLREVRIAESKDETKVGDRVLV
                     ESFKAGELVDVSGVSKGKGFAGGVKRWHYAGGDATHGSMFHRAPGGIGGSSFPSRVWK
                     NQHFPGHMGNERVTAKNLKVIKVDTDENLLLVRGSVPGPRGQYIFIRKSQKAK"
     gene            complement(2302..2634)
                     /gene="rpsJ"
                     /locus_tag="VFR42_01745"
     CDS             complement(2302..2634)
                     /gene="rpsJ"
                     /locus_tag="VFR42_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU0367901.1"
                     /translation="MASLKERIRIRLKAYDHRVLDQSTTEIVDTAKRTGAQVAGPIPL
                     PTIKNKYCVLRSPHVDKKSREQFEMRTHKRLIDILEPTPDTVDALMKLDLPPGVDVEI
                     KAFGHAAK"
BASE COUNT          457 a          880 c          692 g          659 t
ORIGIN      
        1 gttaccgttg cgccgacttc cagacccacc gggtgcagga tgtagcgctt gtcgccgtcc
       61 gcatagtgca gcagtgcgat gttggcgctg cggttggggt cgtactcaat ggcctcaacc
      121 ttcgccggga ccccgtcttt gtcgcgacgg aaatcgactt tgcggtactg cttcttgtgg
      181 ccgccgccgc ggtgccaaac cacgacttcg ccgtggttgt tgcggccgcc ggagcgcttt
      241 ttcggttcca ggagccgctt ttccggcgtc tccttggtaa tctcgttttt gtcgagcacg
      301 gtcataaacc gtcgcgacgc tgtgtatgga ttaaagctct tcagtcccat cgctgcctct
      361 tgctactcaa tctctgcggc ttgcacctgc cgcgtcttgc ttccggtctt tactgcctga
      421 ccccgactta caaatctccg gttacaaatt ctccgctaca agttttcagc aaattcgatc
      481 atcttttcgc cggcggccag ctttacgtac gccttcttcc agtcgcggcg atagccttcg
      541 ttctgcccgc gccgccgcat cttgcccaca aaattcgcgg tacgcacacc ggctaccttc
      601 accttgaaaa tctgctgcac ggcttccttg atctgcgtct tggtggcttt tgcggaaact
      661 tcaaacacca ccgtgccttg cacttctttt acgcccaatc ctttttccgt gatgatcggg
      721 cggcgaataa tctgatagtg aggggtcgac atcgtttatt ccttcccctt tttcgcagcg
      781 gatttgcctc gaggcttggc cggttttttg gcggccggct ttctcgcagc ggcttctttt
      841 ttcaccggag ctgcagcaat cgtttcaacg tctggtacaa ccaccggttt cttttccggg
      901 tcgagcgaac gcaccagacg agccaccgca tccttcgaga gcaccagccg gtcatgcttc
      961 aaaacttcgt acggctgcaa cgcgttcggc gcagacagct tgaccccttc gagattgcgg
     1021 ctggccagtt ccaggttgcg gttttcaccg tgcgaaacca gcaacgcgga cttagtgaag
     1081 ttcaattttt cgagcgacgc cagcaacgac tgcgtcttgt gcgattcgag ctgccaggca
     1141 tccaccacgg tcagcttctg gtcggccagc tttgccgaca gcgccgagcg cagcgcgcca
     1201 agcaactgct tcttcggcaa agcgtaggaa taatcgcgcg ggcgaggtcc gtgaaccgtg
     1261 ccgccatggc gccatagagg cgaccggatc gagccgacac gcgcgcggcc agtgcctttt
     1321 tgccgccaca gcttgcgacc ggcgccggac acttcgctct tttcctttac cttgtgggtg
     1381 ccgcggcgaa gacccgcgag ataccaccgc gacgcttcgt gcagcaggtg cgcattgacc
     1441 ttggcgccga atacggcatc cgccagctcg acttcgccga ctttctttcc gtccagattg
     1501 actacatcca cgactggcat aatctcgttt ccttctgcgt atttctaaag cctgttactt
     1561 cgcactggtc ttgcttcgcc tcagccttac ttggctttct gcgacttgcg aataaaaatg
     1621 tactgcccgc gcggccccgg caccgacccc cgcaccagca gcaggttctc gtcggtgtcc
     1681 accttgatca ctttcaaatt cttggcggtc acccgttcat tgcccatgtg acccggaaag
     1741 tgctggttct tccacacgcg cgacggaaac gaactgccgc caatgccgcc cggcgcgcgg
     1801 tgaaacatcg aaccgtgcgt cgcatcgccg cccgcgtagt gccagcgctt cacgccgcct
     1861 gcaaaacctt tgcccttgct cacgccgctg acatcgacca gttcgccggc cttgaaactc
     1921 tccaccaaca cgcggtcgcc aaccttggtt tcatctttcg actcggcaat gcgcacttcg
     1981 cggagcacct tcatcggcgc cacatccgcc tttttgaagt ggccggtcat cgccttattc
     2041 acgcgctgcg gcttgacgaa ttcgaccagg cccagctgcg cagcgtcgta gccgtctttt
     2101 tccttggtgc ggcgctgcac cacaacgcaa ggcccggctt gcaacaccgt gcagccgacc
     2161 agattgccgt cttcggcaaa aatctgcgtc atgcccagct tgattccgat aatcccgttt
     2221 accgccatgt cctcttctct ctttcgcagc ttgccgcaac ttcccccggt attggctgcg
     2281 tcctgctgaa atttttaacg cttacttcgc ggcgtggcca aacgccttga tttcgacgtc
     2341 cacacccggc ggcagatcga gcttcatcag agcgtctacc gtatccggtg tcggctccag
     2401 aatgtcgatc aaacgcttgt gcgtgcgcat ctcaaactgc tcgcgcgatt tcttgtccac
     2461 gtgcggcgaa cgaagcacgc aatatttgtt cttgatcgtc ggcaagggaa tcggtccggc
     2521 aacctgggcg cccgtgcgct tggccgtatc gacgatttcc gtcgtcgatt ggtccaggac
     2581 ccggtgatcg tacgccttga ggcgaatgcg gatgcgttct tttaaactgg ccatgttgaa
     2641 ccgctattcc aaaatttccg ataccgtgcc ggcgcctacc gtgcggcc
//
LOCUS       DASTPM010000099         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1243170, whole genome shotgun sequence.
ACCESSION   DASTPM010000099 DASTPM010000000
VERSION     DASTPM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243170"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VFR42_01750"
     CDS             complement(<1..344)
                     /locus_tag="VFR42_01750"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0367902.1"
                     /translation="MFAATQDYQQGPDCLHQERAVISRVQDLFDEAQEIQSANTAAPL
                     ATRMRPRSLEEFVGQEHILGPGKLLRRAIEADRLPSVIFSGPPGSGKTTLARVIAEMT
                     HAKFIRISGVES"
     gene            392..>2340
                     /locus_tag="VFR42_01755"
     CDS             392..>2340
                     /locus_tag="VFR42_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU0367903.1"
                     /translation="MKSVSAPRQPFVGLALMAATGIAAAEIVPLRSTVLILAAIALAI
                     CILVALYWPNLAATYLFVAFGFFLLHKYATTNTAGQQLADNLGERPRVVTAVGYVITE
                     PKISPSGFATFLFKLRSIEFEGKNESTHAVWQVRWKGAPDFGDELKLFGTAEPIAPPR
                     NPGEFDIRAFLARHDVRRMLFARYPEDGTLIRHAGGNPVLRAAQASRTWMQNALCRGL
                     DDAPEVKSFITGIVLGIRHESPEDIEEPFQQTGTIHLFAVAGLHVGIVAALLWVLATI
                     AGLPRKRAAAFIIPSLFFYAAVTGLHIPALRAAVMASILVGSYFFERRVFLPNSLAAA
                     AFFILCWNTNELFSTGFQLSFTVVGAIVLFANPFFRILQRRATPDPFLPQSLVRGPRR
                     WMHATNEWLCGSASVSFAAWIGSLPFVLWYFHLVTPISLLANLVVVPIAFFVLAVALL
                     SLTTTPLLPWVALIFNNANWTLATLVIGIVHLFAQIPGGHFYVGEPDWGDRISAKMTV
                     LDLGAGAAVHVRVNGHDWLVDCGSERSYERIVREYLHWAGVNRLTGLVLTHGDSQHIG
                     GVTQLLSDFPPVHVIDNSAPDRSLIHRRLSRIVSGLEGGRRKPSELAAGDNFHLSRHA
                     IAHVLFPPGGFTGPTADDQAMVILVP"
BASE COUNT          439 a          674 c          655 g          572 t
ORIGIN      
        1 ttactttcca caccactgat tcgaatgaat ttggcgtgcg tcatctcggc aatgacgcgg
       61 gccagcgtgg tcttgccgct tccaggcggt ccggagaaga tcactgaggg caaacgatcg
      121 gcttcgatgg cgcgccgcaa gagtttgccc ggcccaagaa tgtgttcctg gccgacaaat
      181 tcctccagcg agcgcggccg catccgcgtc gcgagcggcg cagcggtatt tgcactttga
      241 atctcctgcg cttcatcgaa taggtcctgc acgcgagaaa ttacggcccg ctcctgatgc
      301 aagcaatcgg ggccctgctg atagtcctgc gtcgcggcaa acatattgcg gcttgcgctg
      361 ctaacgcgcg ataagcgaaa actgcgagta gatgaagtcg gtcagtgcgc cgcgtcagcc
      421 attcgttggg ctggcgttga tggccgcgac gggaattgcg gcagcggaaa tcgttcccct
      481 gcgatctact gtattgattt tagcagcgat agctctcgcc atctgcattc tcgttgcgct
      541 ctactggccg aacctggctg caacatatct tttcgtcgct tttggttttt tcctgctgca
      601 caaatacgcg acgacgaaca cggcaggcca gcaactcgca gacaaccttg gcgagcggcc
      661 gcgtgtggtc acagcggtcg gatacgtgat cactgagcca aagatctcgc ccagcggatt
      721 tgcaaccttc ctcttcaagc tgagatcaat tgaattcgaa ggcaaaaatg aatccactca
      781 tgctgtctgg caggtccgtt ggaaaggcgc gccggacttc ggcgatgaac tgaaactttt
      841 tggaaccgct gagccaatcg cgccgccacg aaaccccggc gaattcgaca ttcgcgcgtt
      901 cctcgcacga cacgatgtcc gtcgaatgct cttcgcccgt tatccggagg acggtacgct
      961 gatccgccac gccggcggca atccagtctt gcgcgctgca caagcatccc gcacgtggat
     1021 gcaaaacgcg ttgtgccgcg gcctggacga tgcgcctgag gtgaaaagtt tcattactgg
     1081 aatcgtactt ggcatccggc acgaatcacc ggaggacatt gaggagccgt ttcaacaaac
     1141 gggcacgatt caccttttcg ctgtcgccgg cttgcatgtg ggaattgtgg cggcgctttt
     1201 gtgggtgctg gctacgatcg cgggcctgcc acgcaagcgc gcagccgcat tcatcattcc
     1261 gtcgctcttt ttctatgcgg cagtaaccgg gctgcatatc ccggcgttac gtgcagctgt
     1321 gatggcatca atccttgtcg gcagttactt ctttgaacga cgagtatttt tgccaaacag
     1381 tctcgcggca gccgcgtttt tcatcctctg ctggaacacg aacgaactgt tttccaccgg
     1441 gttccagctt tccttcacgg tggttggcgc aattgtgctg tttgcaaacc cgtttttcag
     1501 aattctccag cggcgtgcga ctcccgatcc ttttctgccg caaagcctag tgcgcggtcc
     1561 acgccgatgg atgcacgcaa caaacgaatg gctttgcggc agcgcatctg tctcgtttgc
     1621 ggcgtggatt ggttcattgc ccttcgttct ttggtacttc cacctggtca caccgatttc
     1681 tttgctggcg aacctcgttg ttgttccgat cgcattcttt gtcctcgccg ttgccctcct
     1741 gtcgctgaca actacgccgc tgctgccgtg ggtcgctctt attttcaata atgccaattg
     1801 gacgctggcc acgctggtca tcggcatcgt tcatctgttc gcacaaattc ccggtggaca
     1861 cttttatgtt ggcgaacctg actggggcga tcggatctct gcaaagatga cggtgctgga
     1921 tttgggcgcg ggcgcagccg ttcacgtccg agtgaatggc cacgattggt tagtcgattg
     1981 cgggagcgaa cgcagttatg agcgaatcgt gcgcgagtat ttgcactggg ccggcgtgaa
     2041 tcggcttact gggttggtgc tcacccatgg cgattcgcag cacatcggtg gcgtcacgca
     2101 attgttgagc gatttcccac cagtccatgt gatcgataat tctgcgcctg atcgttcgct
     2161 cattcatcga cggctgtccc gaatcgtgtc gggcctggaa ggcggtagac gcaaaccttc
     2221 ggagttggct gccggcgaca attttcattt atcccgccac gccattgcac atgttttgtt
     2281 cccgcccggc gggtttacag gaccaacagc ggacgatcaa gcgatggtta tcctggtacc
//
LOCUS       DASTPM010000100         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_291735, whole genome shotgun sequence.
ACCESSION   DASTPM010000100 DASTPM010000000
VERSION     DASTPM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291735"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..648)
                     /locus_tag="VFR42_01760"
     CDS             complement(91..648)
                     /locus_tag="VFR42_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEU0367904.1"
                     /translation="MSTRTTTDRQQFTGSMRFLHWLMAAMVLAMLGIGVAMVASTGDY
                     HRLVSIHRPLGIAILIVVAIRFVNRQFSTLPPFPASMSSEERFVAHASEVLLYTLLFV
                     LPLIGWGMLSAARYPIVLFGSVHLFPILPHSVMLYAVLRRAHKFLAYLLFLAFVAHFG
                     AILFHTLIVRDGMLSRMAPWRARAR"
     gene            complement(645..1790)
                     /locus_tag="VFR42_01765"
     CDS             complement(645..1790)
                     /locus_tag="VFR42_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEU0367905.1"
                     /translation="MAPLGANQDAKTTNGSGELLGETAAMENHPAPLTAGNMFARLAG
                     IGVLLLGVVGAFLYLGGWFSPQKLTPARFVDEFERVGGTHSGFRRNHAKGVCVRGFFD
                     SNGQGARLSKAVVFQSGRVPVIGRFSLGGGDPHTTDELSTVRGLGLQFSLPGEQWRTA
                     MIDLPVFPFKDPQAFYDNLVASQRDPNTHQPDPAKAGAFLASHPETSRALVIIKSHAP
                     SSGFDNTAYYGLNAFRFVNADGGSTYVRWTLVPVQPYEAGTRASQDKNFLFDGLIASI
                     QQHPLQWHLVMTVAQSGDPTDNATIQWPEGRQQVDVGTLTLDSVESEESSPARDINFD
                     PLVLPAGMAPSNDPLLSARSAIYSQSFTRRAGETKEPSEITPSKVRK"
BASE COUNT          454 a          655 c          624 g          394 t
ORIGIN      
        1 aaagtgcgca cgcgcggctt tttcaacgtt gtcctcgcgc ttccatcggt cgtcagcatc
       61 gatgcggcca agaatgccgt ctcgaggaag ttagcgcgcg cgggcccgcc acggcgccat
      121 gcggctgagc atcccgtctc gcacaatcag cgtgtgaaac agaatggctc caaaatgagc
      181 gacgaatgcg aggaatagga gataggcgag aaacttgtgc gccctccgca agaccgcgta
      241 cagcatcacg ctgtgcggaa ggatgggaaa aagatgcact gacccgaaca ggacaatcgg
      301 atatcgcgca gcagacaaca tcccccaccc gatcaacggc agcacaaaca gcagcgtata
      361 caacaatact tcggatgcgt gtgcgacgaa gcgctcctcg gacgacatgc tggcgggaaa
      421 cggaggcaga gtactgaact ggcgattcac gaaacgaatc gcgaccacaa tcagaatggc
      481 gatccccaac gggcggtgaa tggaaaccag gcgatggtag tcgcccgtcg atgcgaccat
      541 cgcaacgcca atccccagca tggccaggac cattgcggcc atcagccagt gcaggaaccg
      601 catggaccca gtgaattgct gccggtcggt cgtcgtccta gtgctcactt tcgcaccttt
      661 gaaggcgtga tttcgctcgg ctctttcgtt tcgcccgctc tcctcgtaaa cgattgcgag
      721 taaatcgccg accgcgcgct cagcaaggga tcgttggacg gcgccatgcc ggccggcaaa
      781 accagcggat cgaaattgat gtcgcgcgca ggactgcttt cctcgctttc cacagaatcg
      841 agcgtcaggg ttcccacgtc cacctgctgc cgcccttctg gccactgaat agtcgcgttg
      901 tcggtcggat caccggactg tgcgaccgtc atgaccaagt gccattgcaa cgggtgctgc
      961 tggatgctcg cgatcagccc atcgaagagg aaattcttgt cctggcttgc cctggttccg
     1021 gcctcatacg gctgcaccgg aacgagcgtc cagcgcacgt aggtggaccc gccgtccgcg
     1081 ttgacgaatc ggaacgcatt gagaccgtag taggcggtgt tgtcgaaacc ggacgacggc
     1141 gcatggctct tgatgattac gagcgcccgc gaggtttccg ggtggctcgc gaggaacgcc
     1201 ccggctttcg ccggatcggg ttgatgcgtg ttggggtcgc gttgcgatgc caccaggttg
     1261 tcgtaaaagg cctgcggatc cttgaacgga aacaccggca gatcgatcat tgcggtccgc
     1321 cactgttcgc cgggcagaga aaactgaagc cccaagccgc ggacggtgct cagctcgtcg
     1381 gtcgtgtggg gatcgccgcc gcccagggaa aagcggccga tcactggcac gcggccgctc
     1441 tggaagacga cagccttcga gaggcgcgcc ccctgcccgt tgctatcgaa aaatcctcga
     1501 acgcatacgc ctttcgcgtg attgcgacgg aaacccgagt gggtgccgcc cacccgctca
     1561 aactcatcga cgaaccgcgc aggcgtcagt ttctgcgggc tgaaccaccc gcccagatag
     1621 agaaacgctc ccacaacacc tagaagcaga acgcctatac ccgccaatcg ggcgaacatg
     1681 tttcctgcgg tcaggggcgc tggatgattt tccatcgctg cggtttcacc caacaactct
     1741 ccgctgccgt ttgtggtttt tgcgtcctgg ttggccccta acggtgccac tgcgccctcc
     1801 cttccctttg gtccggagat agcactttga tgcaaaagta agcaaggaag gcgctttttt
     1861 tgtgtgccac cgtaatcggc cgccgccatg ccctgcggtc gctacttgca cgaacggatg
     1921 gattaagaac cgcctgtatt gattatgtcc cttcgcgttt tgatctacgc aagccgttga
     1981 acagcaccgg gcggacaggg aagtcggaag taatgcaaac ggcggcggcg ccgcaggcct
     2041 ccgttccacg agtggccagc gaagacaaac catgctctaa cgacggaacc gtcggcgagc
     2101 gacccgcacc gcaagctatt ccagcta
//
LOCUS       DASTPM010000101         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1185490, whole genome shotgun sequence.
ACCESSION   DASTPM010000101 DASTPM010000000
VERSION     DASTPM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185490"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFR42_01770"
     CDS             <1..571
                     /locus_tag="VFR42_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367906.1"
                     /translation="DSKDLVSALEKSSSVTLEQKAQLNELLSDETPDEAALALAAADL
                     EMDEERRKTLLQQISAMTVAQRVKFAMKGGADARRVLIRDTNKVVQRAVLQSPRLTDQ
                     EVEAFASMTNLTDEILRLIGKNRNFRKNYSVVRNLLNNGKAPLDVTMNLLPMLNPMDL
                     KRLTMNKNVPETLRTTAVKLQRQRSESKK"
     gene            597..1070
                     /locus_tag="VFR42_01775"
     CDS             597..1070
                     /locus_tag="VFR42_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0367907.1"
                     /translation="MKKLLVLSSAILAASSIFAVRSLRATPEDPWRSKQTVEAADFAK
                     ELQQEKDPYPTVIYVGVKTLYEGGHIPGAVFYGPSSTEPGLADLRKFAATLPKNSDVV
                     LYCGCCPLEKCPNLRPAFSALKDSGFARLRVLLLPTSFNTDWVEKGFPIHKGEKP"
     gene            1151..1696
                     /locus_tag="VFR42_01780"
     CDS             1151..1696
                     /locus_tag="VFR42_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367908.1"
                     /translation="MRQARFMPAILSLCLICFLVIPASAFLQSALPKPDKDTVLKSSD
                     IKARLFPEAVFFRGQSAPVQMRNTGGVHFIDDLYVLAGMVDNSGYSTGIREKYQAYLL
                     SEVTLQIGGQRLKPGAYGMGFIEGGKFVVMDLAANDVLQAASQHDAEMKRPTPLQVVA
                     ADGGKYRLYMGRDYVEFSRTK"
     gene            1770..>2639
                     /locus_tag="VFR42_01785"
     CDS             1770..>2639
                     /locus_tag="VFR42_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367909.1"
                     /translation="MTKTIHVRRAAACLLLFAATTIAAAKPAKLLFDGKSWWDHVKVI
                     ADDSMEGRETGSLGLRKAEAYAVEQLKRAGLQPAGTDGFYQNIQFVQRQIDENNSYVF
                     LMRNGQANPVALGDDAYFSTRVEGSDQEVDSPLVFAGNGLQVPESHLDELAGLDLKGK
                     VVVYLAGSPASVPGSLAAHYGGLAQRWKAYAAAGAVGIVVIPNPASMDIPWSRMSLNR
                     AHPSMDLADPEFNEVAGLKVAMVFNPAAADALFDGSGHSFADVAALGRDRKELPHFAL
                     AVSLRAQAAIRKTT"
BASE COUNT          597 a          633 c          844 g          565 t
ORIGIN      
        1 ggattcgaag gatctggtgt cggctttgga gaaatccagc tcggtgacac tggagcaaaa
       61 ggcgcagctc aatgaactgc tgagcgacga aacgccggat gaggcggccc tggcgctggc
      121 cgccgcagat ctggagatgg acgaagagcg ccggaagacg ttgctgcaac agatttcagc
      181 gatgaccgtg gcacagcgcg tgaaatttgc catgaagggc ggggcagatg cgcgtcgcgt
      241 gctgattcgc gataccaaca aagtggtgca gcgcgcagtg ctgcaatcgc cgcggctgac
      301 ggaccaggaa gtagaagcgt tcgcgtcgat gaccaacctg acggacgaga ttttacggtt
      361 gatcgggaaa aacaggaatt ttcggaagaa ctattcggtg gtgcggaact tattgaacaa
      421 cggaaaagct cccctggacg tgactatgaa tttgctgccc atgctgaatc cgatggactt
      481 gaagaggctg accatgaaca agaacgtgcc ggagacgctg cggaccacgg cggtcaaact
      541 gcaacgccag cgttcggaat cgaaaaagta ggaatccggg gataccttac atgcggatga
      601 aaaaattatt ggttctctcc agcgcgatac ttgccgcatc ttcgattttc gcagtgcgtt
      661 cactgcgcgc aactcctgaa gacccctggc gttcaaagca gaccgtcgaa gcagccgatt
      721 tcgcaaagga gctacagcag gagaaggatc cctaccccac ggtcatttac gttggagtga
      781 aaacacttta tgagggcggg cacatacccg gcgcggtgtt ttatgggccg agttcgacgg
      841 agccagggct ggcggacctg aggaaatttg cggccacgct gccgaagaat tctgatgttg
      901 tgttgtactg cggatgctgt ccgctggaga agtgtccgaa cctgcggccg gcctttagcg
      961 cgctgaaaga cagcggcttc gctcggctgc gagtactcct tctgcccacg agcttcaata
     1021 ccgattgggt ggaaaagggc tttcccattc acaagggcga gaagccgtag ttccttctgc
     1081 ttcgcgcacc gtttttttcc ggcgaatgat gtaccatcta gccgttttgt gaagtgcgac
     1141 gggaggcagt atgcgtcagg ctcgttttat gccggcaatt ctatcgctgt gcttgatttg
     1201 ttttctggtg attccggcaa gtgcttttct acagtcggcg ctgccgaagc cggacaaaga
     1261 cacggtactg aaatcgtcgg acatcaaggc cagacttttt ccggaagcgg ttttcttccg
     1321 cgggcaatcg gcgccagtgc agatgcgcaa cacgggcggg gtgcatttta tcgatgatct
     1381 ttatgtgctg gccggcatgg tggacaactc gggatattcg acgggcattc gggaaaagta
     1441 tcaggcgtat ttgctaagcg aagtgacact gcagattggc ggacaaaggc tgaagcctgg
     1501 cgcttacgga atgggtttta tagagggcgg aaaatttgta gtcatggatc tggcggcaaa
     1561 cgatgttttg caggcggcgt cgcaacacga cgcggaaatg aagcggccca cgccgctgca
     1621 ggtggtggcg gcggatggcg gcaagtaccg gttgtatatg ggacgggact acgtggagtt
     1681 cagccgtacc aaataggtcg acattttcgt cacatcaagc agcagttcag tttcagtgcg
     1741 gaacgctcgc ccaatcgaga gtgcataaca tgaccaagac gatccacgtt cgccgggcag
     1801 ctgcgtgcct gctgctgttc gcggctacaa ccatcgccgc agcaaagccc gcgaagctgc
     1861 tttttgacgg aaagagctgg tgggatcacg tgaaggtgat cgccgatgac agcatggagg
     1921 gccgagaaac cggtagccta ggactgcgga aagccgaagc ctacgcggtg gagcagctga
     1981 aacgcgcagg gttacagccg gcgggcacgg atgggtttta tcaaaacatt cagtttgtgc
     2041 agcggcaaat tgacgagaac aattcctatg tgttcttaat gcgaaatgga caagcgaatc
     2101 ctgttgcgct aggcgacgat gcgtattttt cgacgcgagt ggaaggttcg gatcaggaag
     2161 tggattcgcc tttggtattt gcgggaaatg gattgcaggt tccagaaagc catttggatg
     2221 aactcgctgg cttggacctg aaagggaaag tggtggtgta cctggcggga tcgccggcat
     2281 cggtgcctgg ttctttggcg gcgcactatg gcggattggc gcagcgttgg aaggcgtacg
     2341 cggcggcagg cgcagtagga attgtcgtaa ttccgaaccc cgcttcgatg gatattccat
     2401 ggtcgcggat gtcgctgaat cgggcgcatc cttcgatgga tttggcagat ccggagttca
     2461 acgaagtcgc cgggttaaaa gtggcgatgg tgttcaatcc ggccgcagca gacgcgctgt
     2521 ttgatggatc ggggcacagc tttgcggacg tggcggcgct cggacgtgat cgcaaggagt
     2581 tgccacattt tgcgctggcg gtttcactgc gagcgcaagc cgcgattcga aaaacgaca
//
LOCUS       DASTPM010000102         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_608993, whole genome shotgun sequence.
ACCESSION   DASTPM010000102 DASTPM010000000
VERSION     DASTPM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608993"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..2396
                     /locus_tag="VFR42_01790"
     CDS             366..2396
                     /locus_tag="VFR42_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367910.1"
                     /translation="MNSPSGKIKHVVYVEFDNVHFTRDNPNVPSDLEQMPNLLNFIKQ
                     NGALDTGDHTVLISHTANGILTTQTGLYSDNTSIFIANSFGDFTPSGSALFPSAFFYW
                     TDLVSDITPATQDNTFAMTTPAGLNVPAPWVPFTRAGCDVGAFSTANIVLERQPFDVK
                     KVFGANSDQAKETGGNQTNDFIGVAVHCALNSPLCSQQNNAVADMLPDEPGSYTGYQA
                     LFGMKYVAPALGGFTDYNGNALTGFSALNFNPQPSQTLAVVETMLKKGIPVVFAYIAD
                     AHDNHEGAKLSPAATFGPGEAAYVKQLADYNTAFGTFFANLKAAGIDQSNTLFIFTPD
                     EGDHFSGAAASPAGCDGINVPCTYGVNGVGELDFDLNLAAVNAGDSTPFTYHFDDSAT
                     VYVQGNPDPSSPTVRHLEKTLAGITATNPQTGISESILGTGLGASLQGAIVDKTGQKL
                     LHMSSSVDPNRNPTFTFFGDPNFFFETTGNPNPIVGPGFAWNHGDIQPQIARTFIAMA
                     GPGVRPIGVTQPTDFFTDHVDLRPTMMYLLGLKDDYQHDGRVIMEMVDPNVLPASLHA
                     HTETLLRLGQIYKQINAPFGALAQSTLTVSNFAIQSESDGDAVYASLEAKIAAWTAER
                     DTLCAQTKQMLEAAEFSGVPINEQAAKALIDQSQSLLDRASACAANPAACAN"
     gene            2953..>3299
                     /locus_tag="VFR42_01795"
     CDS             2953..>3299
                     /locus_tag="VFR42_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEU0367911.1"
                     /translation="MTKFWPGNEVRETFLKFFEAKGHRRVRSSSLVPHGDPTLLFTNA
                     GMNQFKDVFLGLEKRDYTRAATAQKCVRAGGKHNDLDNVGHTRRHQTFFEMLGNFSFG
                     AYFKKEAIDFAWAL"
BASE COUNT          709 a         1052 c          858 g          680 t
ORIGIN      
        1 ggctcacaga ctggtgagaa gataaatgac aaaaaaattg caagtatcta cggttccctg
       61 ctgcgcgctg gctttccctc cgacctcatc cgccgcgaac tccaacgcgc gacccgcgaa
      121 gaaattcccg aagcggttca ggacgactcg gaataaccgc ttaggagtcc cgtccgctta
      181 tctttcgtga acctttaatc gcctattcgc ctccgagtca caaaacccgg gtaatcgagt
      241 aacgtccctg gtcttatgtg tcactctgga ggaatagaat gcctggaaat aagtttttgc
      301 tccgcgcaat cggttgcgga ctacttgcgc ttggtctcgc cgcctcggct cggtcgcagt
      361 gtcaattgaa ctccccatcc ggaaaaatca agcacgtcgt gtacgtggaa ttcgataacg
      421 tacactttac gcgcgacaat cccaacgtcc cgtccgatct cgaacagatg cccaacctgc
      481 tgaatttcat caagcagaac ggcgctctcg ataccggcga ccacactgtc ctcatctcgc
      541 acaccgccaa cggcatcctc accacgcaga ccggcctgta ctccgacaac accagcattt
      601 tcatcgcgaa cagttttggc gatttcactc catccggctc agcgctcttc ccttctgcct
      661 ttttctattg gacggatctg gtctcggata tcacgccagc cacgcaagac aacaccttcg
      721 ccatgaccac gcccgccggc ctgaacgttc ctgcgccctg ggttcctttc acccgcgcag
      781 gctgcgacgt cggcgctttc tccactgcca acatcgtgct ggaacgccag cctttcgacg
      841 tgaaaaaagt gttcggcgca aattccgacc aggcaaagga aaccggcggc aaccagacca
      901 acgacttcat cggcgtggcg gttcactgcg ctctcaacag tccgctctgc tcgcagcaga
      961 acaatgccgt cgccgacatg ttaccggacg aacccggcag ctacaccggc tatcaggcgc
     1021 tcttcggcat gaagtacgtg gcgcccgcgc tgggcggctt caccgactac aacggcaacg
     1081 cgcttaccgg tttcagtgcc ctcaatttca atccgcagcc gtcccagacc ctcgcggtgg
     1141 tcgagacgat gttgaagaag ggcattccgg tggtcttcgc ctacatcgcc gacgcccacg
     1201 acaatcacga gggagcaaag ctcagccccg cggccacttt tgggccaggg gaagccgcct
     1261 acgtgaaaca actggccgac tacaacaccg ccttcggcac tttcttcgcc aacctcaagg
     1321 ccgccggcat cgaccagtcc aacacgctgt tcattttcac tcccgatgaa ggcgaccact
     1381 tctccggcgc agcggccagc cccgcgggtt gcgatggcat caatgtcccc tgcacgtacg
     1441 gcgtaaacgg cgtaggtgaa ctggacttcg acctcaacct cgcagcggtt aacgctggcg
     1501 acagcactcc gttcacctac cacttcgatg attccgccac ggtgtatgtg caaggcaatc
     1561 cggatcccag ctcgccgact gtacggcacc tggaaaagac tctggccggc atcaccgcca
     1621 cgaaccctca gaccgggatc agcgaaagca ttctggggac ggggctcggc gccagcctgc
     1681 aaggcgccat cgtggacaag accgggcaaa agctgctgca catgagttcc agcgtggatc
     1741 ctaaccgcaa tccgacgttt accttttttg gcgatcccaa cttcttcttt gagaccaccg
     1801 gcaatcccaa tcccatcgtg ggtccaggct ttgcctggaa tcacggcgac attcagccgc
     1861 agatcgcgcg cacttttatc gccatggcag ggcccggtgt tcgccctatt ggcgttactc
     1921 agcccaccga tttcttcacc gaccacgtcg acttgcgccc aaccatgatg tatttgctcg
     1981 gtctaaagga cgactaccag cacgacggcc gcgtcatcat ggaaatggtg gatcccaatg
     2041 ttttgccggc cagcctacac gcgcacacgg aaacgctgct tcgtctcggc caaatctaca
     2101 aacagatcaa cgctccgttc ggcgctttgg cgcaaagtac cctgaccgtt tccaatttcg
     2161 ccattcaaag cgagtcggac ggcgacgccg tgtacgccag cctggaagca aagatcgctg
     2221 cctggaccgc cgaacgcgac accctgtgcg cacaaaccaa acagatgctg gaagcagcgg
     2281 aattttccgg tgtacccatc aacgagcagg ccgcaaaagc gctcatcgac cagagccaat
     2341 cgttgctcga tcgtgccagc gcctgtgcgg cgaatcccgc cgcctgcgcc aactgagcat
     2401 ttcctggcct gtcggcgcca accgatttgg cgccggcgga ttccgtgggg cgaatgcgtt
     2461 tctatgggtt cgccccattt ttgtgcttcc ggactctggg ccatacctga ctaagccctc
     2521 gcgccgagcc ttggttcgag agcgtggtat taactgtatt gctaatttgt gctaagttta
     2581 gtgaatcgct caacccgcta agttcctgcc gcacatccgg gaaaatcgcc tcctgagttc
     2641 tttcgcgcgc tgtaagcctt aaagggacac ctacgaaaaa tggctttgta agtcctttgt
     2701 ttgccacatc cgaaaaaacc acagggagtt agatgggcgt tccatttttg gtgcggactt
     2761 cagtaggtgc gcggtgtcaa cccgcaggcg gccgcagacg gtcggacgcg gcccgtcgcg
     2821 ctggccactt tcttccgggg tctgcgataa tggatagctc taaatcagtt ccggcgaggt
     2881 ctcatcgccc ggctagggcg cagagaacaa gatgacggtt gaagctacta agacgtgccc
     2941 ggtgagggca cgatgacaaa attctggcca gggaacgagg ttcgcgagac cttcctcaag
     3001 ttctttgagg ccaagggcca tcgccgtgtg cggagcagct cgctggttcc tcatggcgat
     3061 cctacactgc tgttcaccaa tgccgggatg aaccagttca aggacgtttt cctgggtctg
     3121 gaaaaacgcg actacacgcg cgccgccacc gcgcagaaat gcgtgcgcgc cggcggcaaa
     3181 cacaacgatc tcgacaacgt cggccacacg cggcggcatc aaacgttttt cgagatgctt
     3241 gggaactttt ccttcggagc gtatttcaaa aaggaagcaa ttgattttgc gtgggcgct
//
LOCUS       DASTPM010000103         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1156634, whole genome shotgun sequence.
ACCESSION   DASTPM010000103 DASTPM010000000
VERSION     DASTPM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156634"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..890)
                     /locus_tag="VFR42_01800"
     CDS             complement(36..890)
                     /locus_tag="VFR42_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0367912.1"
                     /translation="MASVTNPVVPRTEEIRFMPYITMSGILHGGLIVLIALSAYFHWN
                     GNRWAGPGGGSENVTNVKLVGNSGMPLPTPPSLNESNVVDPSKSLWKNDIKPEPPQLK
                     KEEIVPPDLKNVPEFKTLEKNNKKPPPPKKENKAFQPKQPNPDNAVPGPNLGAMKVPT
                     GAGTQPGSSSTPGMAIKAEGGGDFASRYGWYIEAVKRRIQTNWLQNTIDPGVRAAHTA
                     HAVVEFTIYKDGTVKDIHISQTSGNLSMDNSGLRAIMSSNPMPGLPPDYAGSNVRVIF
                     DFDLSMTR"
     gene            complement(896..1312)
                     /locus_tag="VFR42_01805"
     CDS             complement(896..1312)
                     /locus_tag="VFR42_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0367913.1"
                     /translation="MPQLMGKTQSSLSEINMVPFIDVVLVLLIIFMITAPILQSGIEV
                     DVPKTKTVKEITEQRLVISVDKNQLIYLGNSPVNIHDLGAKVQAQLKKPQDAVFLRCD
                     EKVPFGVFASVIDALRQAGINNISIVTQPLTDKSRG"
     gene            complement(1325..1897)
                     /locus_tag="VFR42_01810"
     CDS             complement(1325..1897)
                     /locus_tag="VFR42_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0367914.1"
                     /translation="MIFQKLGLFGRVHRQSDQFLRIFRATRGVANPQALATAGSPFAN
                     VYAAGYRELQFQVGTHPGNPEPPRLKSLSAVTVAMQLASAEEVRRVEKGMSWLATTGS
                     VTPFIGLFGTVWGIMDAFTGLGDAGSASLRAVAPGIAEALVTTAMGLFAAIPAVIFYN
                     NFVGDIRDLGQRLDTFALEVTAQIEKTFVG"
BASE COUNT          487 a          725 c          571 g          530 t
ORIGIN      
        1 cttggtctct ggcggctggg aagggaactc gccatttatc ttgtcatact aaggtcgaaa
       61 tcgaaaatca ctcttacatt ggacccggcg tagtccggtg gcagacccgg catgggattc
      121 gacgacatga tcgcccgtag ccccgaattg tccatggaaa gattcccgct ggtttgcgaa
      181 atgtggatgt ctttcaccgt tccatctttg taaatcgtga actccactac tgcgtgcgcc
      241 gtatgcgccg ctcgcacgcc cggatcgatt gtgttctgca gccagttggt ctgaatccgg
      301 cgttttaccg cctcgatgta ccagccataa cgcgacgcaa aatctccgcc gccctcggcc
      361 ttaatggcca ttcccggcgt gctggatgat cccggctgcg taccggcgcc cgtcggcacc
      421 ttcattgcgc ccaagttagg ccccggcacg gcattgtccg gattcggctg ttttggctgg
      481 aacgccttat tttccttctt cgggggaggc ggcttcttgt tgttcttttc cagtgtcttg
      541 aattccggca cgttcttcaa atccggcgga acaatctctt ccttctttag ctgcggcggt
      601 tccggtttga tgtcgttctt ccacaaactc ttcgatggat ccaccacgtt gctttcgttc
      661 aacgacggcg gcgtaggcag aggcattccg ctgttgccta ccaatttcac attggtgacg
      721 ttttcgctcc ctccgccggg gcccgcccag cggttcccgt tccaatgaaa ataggccgaa
      781 agcgcgatta gcacgatcag tccgccatgc agaatcccgg acatggtgat gtacggcatg
      841 aagcggatct cttccgtgcg ggggaccacc ggattggtaa cactggccat tttttctatc
      901 cgcgactttt gtccgtcagt ggttgcgtca cgatactaat attattgatg ccggcttgcc
      961 gcagcgcgtc gatcaccgac gcgaacaccc caaacggaac cttctcgtcg cagcgcagaa
     1021 ataccgcatc ctgcggtttt ttcaattgcg cctgtacctt cgcgcccaga tcatgaatgt
     1081 tcaccgggct gtttcccaga taaatcagct ggttcttgtc cacgctgatc accagccgct
     1141 gctccgtaat ttcctttacc gtcttcgtct tcggcacgtc cacttcaatg cccgactgca
     1201 gaatcggcgc ggtgatcatg aagataatca gcagcaccag caccacgtcg atgaacggga
     1261 ccatgttgat ctccgacagc gacgactgtg ttttccccat caattgcggc ataaactcag
     1321 cgccttatcc gacaaacgtc ttttcaatct gcgccgttac ttccagcgca aatgtgtcca
     1381 gcctctgccc caggtcacga atatcgccca caaagttgtt gtagaaaatc accgcgggaa
     1441 tcgcggcgaa caaccccatt gccgtggtaa ccagcgcttc ggcaatgcca ggcgccactg
     1501 cacgcaagct ggccgagccc gcatcaccca gtccggtaaa cgcatccata attccccaaa
     1561 ccgtgccgaa caatccgata aacggagtca ccgacccggt cgtcgccagc cacgacatgc
     1621 ctttctccac gcgccgcacc tcctccgcgg aagccaattg catcgccacc gtcacggcac
     1681 ttaggctctt caacctcggc ggctccggat ttcccgggtg ggttcctacc tgaaattgca
     1741 gctcgcggta tcccgccgca tacacattcg cgaaaggcga gcccgctgtc gccagcgctt
     1801 gcggattggc gactccgcgc gtggctctga aaatccgcaa gaactgatca ctttgccggt
     1861 ggacgcggcc gaacaggcca agcttttgga agatcatgcc ccacgaaacc acggagaaaa
     1921 ataacagaag gcctaaaacg ccgcgcgcga cccaggtcgt ttgttccaga atctttccaa
     1981 gatcgacggc gaaggctgca atcagaaaaa aggaaatgtt cgtccctcag tttgagaaaa
     2041 tttgctggtc tttagcaacg taacacacgc tcggagtcga atcaacgaat cacgctctgc
     2101 gtgccgttgt ggtcaccgcc tcgaaagagg cccgcaatca cccgattgct agagtcttaa
     2161 ccttcaggtc agaacgattt ccggctcgcc agaggtcaca aattgccatt tgctaccgta
     2221 ctctcagcac gcgcagaaca agcatccagg tacgcgcacg caaccacgat caactctcgg
     2281 cggaaaatgt ctccttcctt ctcgggagcg ccc
//
LOCUS       DASTPM010000104         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_666630, whole genome shotgun sequence.
ACCESSION   DASTPM010000104 DASTPM010000000
VERSION     DASTPM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666630"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /gene="selA"
                     /locus_tag="VFR42_01815"
     CDS             complement(<1..811)
                     /gene="selA"
                     /locus_tag="VFR42_01815"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU0367915.1"
                     /translation="MDSRAKRALTADQTDLLRQIPSVDELLAQPRLATLAQRVDRELL
                     VEITRTVLAEVRGRIAGEDAAVVALDSFSLEERIASMVERVLESSLQAVINATGVILH
                     TNLGRAPLSEAAADEIRRTAIAYNNLEYDLEKGARGKRDVHVADMLARLTGAEAAIVV
                     NNCAAAVLLVLAALAKGGEVLVSRGELIEIGDGFRIPEIMAQSGAMLREVGTTNRTRV
                     ADYENAINENTRLLLRVHPSNFTITGFAEKPGLNELVSLGQRTGLPVVEDLG"
     gene            959..1561
                     /locus_tag="VFR42_01820"
     CDS             959..1561
                     /locus_tag="VFR42_01820"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEU0367916.1"
                     /translation="MATIDEEMSQLEKDIRQLKIEYDQFFGGGRKRPPTEIEWRIELI
                     IKRYGERGGEMKFGQRFRYNNLTQTYAKYKDIFRKKTKQREEGTVQRHFGAAAKAIEA
                     ERAKAAAASTRPAAPEPAAFRMVCSEPESETDKVDQLYQAFQQAMQETGGSVKQSREN
                     FNEFVSKKTKELQKQKNCREVEYVVEVVNGEVKLKALVKT"
     gene            1671..>2539
                     /gene="purH"
                     /locus_tag="VFR42_01825"
     CDS             1671..>2539
                     /gene="purH"
                     /locus_tag="VFR42_01825"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF013931.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0367917.1"
                     /translation="MAVQRALISVFDKTGIVDFAKKLAGLRIEILSTGGSAKTLREAG
                     LAVRDVSDFTGWPEMLGGRVKTLHPKVHGGLLFRRNLAEDQKQAADHGIAPIDLVVVN
                     LYPFEATAAKRDLTAEELIENIDIGGPTMLRSAAKNFESVTVITDPADYDRVAQELES
                     SQDTSLATRLELARKVFAATSRYDGLIARELERLSASTSHVVLTEKPLLPERLHLALR
                     RQQELRYGENPHQAAALYIPAGRPLHGLAAAQQLQGKELSYNNFVDLEAARSLAAEFR
                     QPAAVIIKHNNPCG"
BASE COUNT          568 a          838 c          631 g          502 t
ORIGIN      
        1 aacccaaatc ctcgaccacc ggcaaacccg tgcgttgccc aagcgacaca agttcattca
       61 gccccggctt ttccgcgaag ccggtaatcg taaagtttga aggatgcact cgcagcaaaa
      121 gccgcgtgtt ctcgttgatc gcgttttcat aatccgcgac acgcgtgcga ttcgttgtcc
      181 ccacttcgcg cagcatcgct ccgctttgcg ccatgatttc cggaatgcgg aagccgtccc
      241 cgatttcaat cagctcgccg cgcgaaacaa gcacctcgcc gccttttgct agggccgcca
      301 acaccagcag caccgctgcg gcgcagttgt ttaccactat cgctgcctca gccccggtca
      361 aacgggcgag catgtctgcc acgtgaacgt cgcgtttgcc gcgcgcgccc ttctctaggt
      421 catattcaag gttgttataa gcgatcgcgg tgcgcctgat ctcgtcggcc gcggcttcgc
      481 ttagcggagc tcgtccgaga ttcgtgtgca agatcacccc ggtggcattg atcaccgcct
      541 gcagcgaaga ctccagcact cgctccacca tggaggcaat gcgctcttcc agcgagaaac
      601 tatccaacgc caccactgcc gcatcctctc cggcgattcg ccctcgcact tccgccaaaa
      661 ctgttcgcgt tatctctaca agcagctcgc ggtccacgcg ttgcgcaagc gttgccagcc
      721 gcggttgcgc caacaattca tccacggacg gaatctgccg cagcaaatcc gtctgatccg
      781 ccgttagcgc ccgtttggca cgagaatcca tgccgccatt tcaacataca gacgcggagc
      841 ccgcaacgct tggtcaccca agggccgacc gtacccgatc agccgctcgc ggcgtctcat
      901 tttcttgacg ccctgcccgg ctcctcctag catgggagaa tcgggggcat cctcagttat
      961 ggcgacgatc gacgaagaga tgtctcagct cgaaaaagac attcgccaac tcaagatcga
     1021 atatgaccag ttttttggtg gaggcagaaa gcgcccgccc accgaaatcg aatggcgcat
     1081 tgaattgatc atcaagcgct acggcgagcg cggtggcgag atgaagtttg gccagcgctt
     1141 ccgctacaac aacctcacgc agacgtacgc gaaatataaa gacatcttcc gtaagaagac
     1201 gaagcagcgg gaagagggaa ctgtacagcg tcacttcgga gccgcggcca aggcaatcga
     1261 agccgagcgc gccaaagccg ctgctgcttc taccaggccc gccgcccccg aaccggccgc
     1321 ctttcgaatg gtttgctctg aacccgagtc cgagaccgac aaagtcgatc agctatatca
     1381 ggcctttcag caagctatgc aggaaaccgg cggcagcgtt aagcaatcgc gcgaaaattt
     1441 caatgaattc gtcagcaaga aaaccaaaga attgcaaaaa cagaaaaatt gccgcgaagt
     1501 cgaatacgta gtggaagtcg tcaacgggga agtaaagctc aaagccctcg tcaaaaccta
     1561 gccggcacat ccttctgcgc cgtatctgga ctcttttcca ccccgccgtt tcctctttcc
     1621 ctcaagccgt gccacgctta cactgttcta cttttccgga ggcagcatac atggcggtgc
     1681 agcgcgcgct catcagtgtt ttcgacaaaa caggcattgt ggacttcgcc aagaaactcg
     1741 ccggcctgcg catcgaaatt ctttccaccg gcggcagcgc caaaaccctc cgcgaagccg
     1801 gcctcgctgt ccgcgatgtt tccgatttca ctggctggcc ggaaatgctt ggcgggcgcg
     1861 tgaagactct ccatcccaag gtccatggcg gcctcctctt tcgtcgcaat cttgccgaag
     1921 atcaaaagca agccgcggat cacgggatcg ctcctataga cctggtcgtc gtaaatctat
     1981 atccgttcga agccaccgcg gcaaaacggg atctcaccgc cgaagagctc atagaaaaca
     2041 tcgatatcgg cggccccaca atgcttcgct ccgcggcgaa gaattttgaa agcgtcacgg
     2101 tgataaccga tcccgccgac tacgatcgcg tggcgcagga actcgaatct tcgcaagata
     2161 cgtcattggc aacccgcctc gagctcgccc gcaaagtctt tgctgccacc tctcgttatg
     2221 acggcctcat cgccagagaa ctcgagcgcc tttccgcctc caccagccac gtcgtcttga
     2281 ccgaaaaacc acttcttcca gaacgcttgc accttgctct ccgccgccag caggagcttc
     2341 gttacggtga aaatccgcac caggctgccg cgctctatat ccccgccggc cgtccgctac
     2401 atggtcttgc cgccgcccag caacttcaag gcaaggagct gtcctacaat aatttcgttg
     2461 acctcgaagc cgcccgcagt ctcgccgcgg aatttcgcca acccgctgcc gtcatcatca
     2521 agcacaacaa tccttgcgg
//
LOCUS       DASTPM010000105         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_983709, whole genome shotgun sequence.
ACCESSION   DASTPM010000105 DASTPM010000000
VERSION     DASTPM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983709"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VFR42_01830"
     CDS             complement(<1..1072)
                     /locus_tag="VFR42_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0367918.1"
                     /translation="MASSAEAGQVSHGKGTNLYQGMPALLEKTQRHAATWFASLAERA
                     ARATVSSDELRGLLGGPLPQRAGDADSVVEALANAGMRGTVATAGPRYFGFVVGGSVP
                     AAVAADWLVSSWDQNCGIYVLSPLVSVIEQITGSWLRELAGLPLTMSFGFVTGCQMAN
                     FTALAAARHRVLQNAGWNVETNGLFGAPPIEVFVGEEAHYTIAMALRLLGLGAERVRR
                     IPTDGQGRMRADQLKAAMRGHAAQKGPCMVCAQAGNVNTGAFDPLAEIAEIAKERGAW
                     LHIDSAFGMWAAVSSMSSARLRGAGHERASLVRGIEKADSIATDGHKWLNVPYDCGIV
                     FCADEAAHRAAMSLAAAYIVATG"
     gene            complement(1346..1936)
                     /locus_tag="VFR42_01835"
     CDS             complement(1346..1936)
                     /locus_tag="VFR42_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0367919.1"
                     /translation="MSTIGERKWSRGMLFAVAASASLLVIGAVYAAIPLELWVGTSNH
                     IGLWKGPSTLTARTLKTPIGETTGAWHYHPGFVYNVVRQGTITVEDGCGNVQSYSAGQ
                     AFETSEGRVHRAYNLGGADAIESNMFVGPPGRPITVPIPNNERRCGPPSTVDECKGDG
                     WASFDFPNQFPNQGACVEFVNHRPRITVLVPEDPLR"
     gene            2204..>3559
                     /locus_tag="VFR42_01840"
     CDS             2204..>3559
                     /locus_tag="VFR42_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0367920.1"
                     /translation="MTSPPLRFQDFELDPAQFELRRAGHRVRLERKPLELLILLAEKR
                     GNLVSREEMVQRVWGDDVYFDAERGINNAVRKIRAALHDESAQPQFVETVVGKGYRFI
                     APVNGGRAETTTGATAATPPPSVGAPAALAVPSVVATHGESTHLSWVSRTSHRRRLLA
                     ASGLFLAILAVVFALTVGRARQRLFASSSPPRIQSLAVLPLKNLSGDPTQEYLADGMT
                     EALIGRLSTIHGLRVISRTSVMSLKDTKMSAPEIAKTLQVDALVEGSVIREGSRIRVY
                     TQLIRGATDEHFWSETYDRELPDVLALESDVAQSVARKVEVTVTGEEHTRLATAHQVS
                     PEVYESYLKGQFSRSNSRADIEQRIAYFKDAIAKDPTFAPAYVGMAGAYGELGGSFTG
                     APTQETRPKVVRMAQKALELDPGLAEPHVLLGEILKKQFRWAEAEAEYKQALALNPNN
                     PA"
BASE COUNT          707 a         1166 c          956 g          730 t
ORIGIN      
        1 ttcctgtcgc cacaatgtac gctgccgcca gcgacatcgc cgcgcggtgg gccgcttcgt
       61 ccgcgcaaaa cacgattccg cagtcatacg gaacgttgag ccatttgtgc ccatcggtcg
      121 cgatggaatc ggccttttcg attccgcgca ccagagaagc ccgttcgtgt ccagcgccgc
      181 gtagtcgtgc gctagacatg gaggagaccg ctgcccacat cccaaacgca ctatctatgt
      241 gcagccaggc gccgcgctcc tttgcgattt cagcgatctc cgccagcgga tcgaacgcgc
      301 ctgtattcac gttgccagcc tgtgcgcaga ccatgcacgg ccctttctga gcagcgtgac
      361 cacgcattgc agccttcaac tgatcagcgc gcatccggcc ctgaccgtcc gtaggaatgc
      421 gccgaactct ctctgcgccc agtcccaaca atcgcagcgc catcgcgatc gtgtaatgcg
      481 cttcttcccc cacgaatact tcgatgggcg gagcgccaaa caatccgttg gtttcgacat
      541 tccatcccgc gttctgcaac actcggtggc gcgccgcagc cagtgccgtg aagttcgcca
      601 tctggcagcc ggtcacaaaa ccaaaactca tcgtcagcgg aagtcccgcc agctcgcgca
      661 accatgagcc tgtaatctgt tcgataaccg agaccagcgg agacaacacg tagatgccgc
      721 agttctgatc ccaggacgac acaagccagt cggcggcaac cgcggcagga acgctgcctc
      781 ccactacaaa accaaaatac cgcggccccg ccgtcgcaac cgtcccccgc attccagcat
      841 ttgcaagcgc ctcaacgacg ctgtcagcgt cacctgcgcg ctggggaagt ggcccgccca
      901 gcaatccgcg cagctcgtcg gacgacaccg tcgcccgtgc tgcccgctcc gcaagcgacg
      961 caaaccacgt tgctgcgtgc cgctgcgtct tctccagcag ggccggcatg ccctgataca
     1021 gattcgtccc cttcccatgc gaaacttgtc cagcttccgc ggaactagcc atgagccacc
     1081 tctcgacgcg acgaaaaacc ctgaagccgc ctttttagaa agcgggcttt cgtcggtagc
     1141 gtgggtgggt tcaaccgcca gtgcgacagc caaaatggtt gacggcactc agattgcttc
     1201 gccgtcttgc tgcggtctag cagggtctca caagaaaccc gggggcgacc ctggcggtct
     1261 cccctgagct ctcttcaatt tgttatgaag gcagggattt ttgagccccc cgcgcatgct
     1321 gtttcgtcag agcagctgaa ggccgctagc gcaggggatc ctcgggaacc agcaccgtga
     1381 tccttggccg atgattgacg aactccacgc aagccccttg atttgggaat tggttcggga
     1441 aatcaaaact tgcccaccca tcacccttgc attcgtcgac ggtgctcggc ggaccgcaac
     1501 ggcgctcgtt gtttggaatg ggcaccgtta tcggtcggcc cggaggacca acgaacatat
     1561 ttgactcgat cgcatccgca cctcccagat tgtacgctct gtggacacgc ccttcggaag
     1621 tttcaaaagc ctgccccgcg ctatacgatt gcacattgcc gcatccatct tcgacggtga
     1681 tcgtgccttg tcttacgacg ttgtagacaa acccggggtg ataatgccat gcacccgtcg
     1741 tttcaccgat cggcgtcttg agcgttctgg ccgtcagcgt tgacggacct ttccacaatc
     1801 caatgtgatt gctcgtacca acccaaagtt ccaacggtat cgcggcgtaa acggcgccaa
     1861 ttaccaacaa gctggcactg gcagccaccg cgaatagcat tccgcgagac cacttcctct
     1921 ccccgattgt gctcattgca tttctccttt gttcctttgg ttttgcaaag acccgccggc
     1981 cgtccggcgt tactcgaact ctcgtgctgg gggctcacgg tgttgaccga aagtcgcgag
     2041 ctggtcgcaa ctctcgtcgc agctccgccg cccgtcaatc acgcgcgggt tttgtcttcc
     2101 tcacgtttgt gtatcggtct aagtccttca agacattcag gcatccgccg ccacgccatt
     2161 gactttcgag atataatccg cgcaagtatc cgcggcgttc cctatgacct cgccacccct
     2221 acgtttccag gactttgagc tcgaccccgc tcaattcgag ctgcgtcgcg ccggtcaccg
     2281 cgttcggctc gaacgcaaac ctttggaact cctcatcctg cttgcggaaa agcggggaaa
     2341 cctggtgtct cgggaagaga tggttcagcg cgtgtggggc gacgacgtct atttcgacgc
     2401 cgaacgcggc atcaacaatg ccgttcggaa aattcgtgcc gccctccacg acgagagcgc
     2461 acagccgcag ttcgtcgaga ctgtcgtcgg caaaggctat cgctttattg cgcccgtgaa
     2521 cggtggaaga gccgaaacaa cgactggagc gaccgctgca acgccacccc cgagcgtcgg
     2581 cgcacccgca gctcttgctg tgccctctgt ggtggccacg cacggcgaat cgactcatct
     2641 ttcttgggtt tcccgcacca gccataggag gcgactcctc gcggcatcgg gccttttcct
     2701 ggctatcctg gcggttgtct ttgctctgac cgtaggccgt gctcgccaac gccttttcgc
     2761 ttccagttcc ccgccgcgca tccagtcgtt ggcggtcttg ccattgaaga atctgtcggg
     2821 cgatcccacc caggaatacc tggccgacgg catgaccgaa gccctcatcg gacgcctttc
     2881 tacgattcac ggccttcgcg ttatttcccg cacttcggtg atgtccctca aggacaccaa
     2941 gatgtccgca ccggaaattg ctaaaacgct tcaagtggac gccctcgtcg aaggttccgt
     3001 catccgcgaa ggcagccgga ttcgtgtcta cacgcaatta atccgtggcg cgaccgacga
     3061 acatttctgg tccgaaactt acgatcgcga actgcccgac gtgcttgctc tcgaaagcga
     3121 cgtcgctcag tccgtggcga ggaaagttga agtcaccgtc accggcgagg agcacacccg
     3181 cctcgccacc gctcatcagg tgtcccccga agtttatgaa agttatctca aaggacagtt
     3241 ttccaggagc aacagccgcg cggacatcga gcagaggatc gcatacttca aagacgcgat
     3301 tgctaaggac ccgacgtttg caccggctta cgtgggcatg gcgggcgctt acggagaact
     3361 aggcgggtcg ttcaccggcg ctccaacgca ggaaactcgt ccaaaggtgg ttcgcatggc
     3421 gcagaaggcg ctggaattgg atccggggct tgcggagccg cacgtgctgc ttggcgaaat
     3481 tctgaagaag caatttcgct gggcggaggc tgaggccgaa tacaagcaag ccctcgcttt
     3541 gaatcccaac aatccggct
//
LOCUS       DASTPM010000106         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_291823, whole genome shotgun sequence.
ACCESSION   DASTPM010000106 DASTPM010000000
VERSION     DASTPM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291823"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..1023
                     /locus_tag="VFR42_01845"
     CDS             412..1023
                     /locus_tag="VFR42_01845"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0367921.1"
                     /translation="MQRRQHPRVRLRMPARLRWSAPLGQRIEACETINASRGGLLLAC
                     GEAPSAGHPLWVTFPFDAEARGMQPEVLARVVRSVPNREDAGTQWKVGLQFERTAHPA
                     LSGNHSTDKKNGAGRNIALPIRVRPRSMPWHEEAMTMEVSPEKLKFVTHREYSFGQQL
                     LVSFDARSDAPWAGDQEWEGQVTGIEMDADSDSVTVTVRKKSA"
     gene            1060..1731
                     /locus_tag="VFR42_01850"
     CDS             1060..1731
                     /locus_tag="VFR42_01850"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU0367922.1"
                     /translation="MAPLMSQGHQSKNFFLEGPAGKLEAVLWTPHGDTPPKNTALVCH
                     PHPLFGGTLHNKVVYQAAKALDGLGMPVLRFNFRGAGLSEGKHDRGRGELDDVRSAVS
                     FLATEFPGTPLLLAGFSFGCWVGLRVACGDPRVERLIGIGAPVNSSDFSYLQQCSKPK
                     LFVHGSHDEHGEVNKVRAMVASLPGENDLVVVEGADHFFTGKIEQLGAAISTWGDRGH
                     HRHGI"
     gene            1793..2233
                     /locus_tag="VFR42_01855"
     CDS             1793..2233
                     /locus_tag="VFR42_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367923.1"
                     /translation="MFDKYLPYWMTRGILRAGTVMAVILFFISYIVDVTLAGVGILPS
                     STVLNDVAIALIAAAVLMFYLFSSRTEQIFLRARERMHLTAELNHHMRRVLSEMRNAA
                     EVENRAERMHMMDHAIEEADHLLIDLVPTVSAERSPRIRSFENR"
     gene            complement(2257..3366)
                     /locus_tag="VFR42_01860"
     CDS             complement(2257..3366)
                     /locus_tag="VFR42_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0367924.1"
                     /translation="MSEKATNDGTRAYAERFHAHAADGHFRETQGLVLSSLGIGTYLG
                     QPDERTDAGYTSAIVAAVENGINVVDAAINYRFQRSERSVGAALAQLEDKGYVREQIV
                     VCTKAGYLTPDAKLPADPNRYFFEEYIQRGIFQAKDISAGSHCMAPRFLQDQLARSLA
                     NMGVDSIDVFYLHNPETQLTEIPKPQFLSRVRDAFHFLEATVRSGKIQFYGMATWNGF
                     RQEAAVRDAMQLSDFVAIAKEIAGDGHHFRFVQLPFNLGMTEALTLGNQSVAGKTLTI
                     MEAAEELNITLIASASLLQGQVVSNLPSFVADALGLESDAERALQFVRSSPGITTALV
                     GMSREQHVLANARLVRIPPATIDQFSQLFSRGQAG"
BASE COUNT          799 a          953 c         1087 g          709 t
ORIGIN      
        1 ataaaggccg cggtagctga cgtcgcgggt gaaggctttc agctcgtgcg cgttcgaatc
       61 gagcgaaaga atcttcaacg gcaaggtcat gttgaaccgg cgagcttcgc ggcggtcggt
      121 cccttcagac ataccactcc ctgtggctga gaaaaaacgc aggcaaaacc tatcatcagc
      181 gcgggtaaaa ccgcaatccg aaattcgctg aatttggctc tagtacttac agaagaacca
      241 tgcaagtact aaacccgtgc ggctttgcgg cgcggcgggt ggaaaggtac tttctgcaag
      301 gtctagtacg gaaccttgcg gcactcgttc cagcgggaat atactccgca cagacccggc
      361 tgaaagcgct gaccccgcaa gaagatgacg tagtgagatg ggcgtgatga gatgcagcgc
      421 cgccaacatc cccgagtgcg actgcggatg cccgcaaggt tgagatggtc cgccccgctg
      481 ggccaacgta tcgaagcatg cgaaacgatc aatgcatcgc gcggcggttt gctgctggca
      541 tgcggagaag cgccaagcgc aggtcatccg ctgtgggtga cgtttccgtt cgacgccgaa
      601 gcgcgcggga tgcagccgga agtcctcgcg cgtgtggtgc gcagcgtgcc gaacagggaa
      661 gacgcgggga cgcagtggaa agtagggttg caatttgaac gaacggcgca tccggcactc
      721 tccggcaacc acagtaccga caagaaaaat ggagcgggac gcaacatcgc gctgccaatt
      781 cgcgtaaggc cgcggagtat gccatggcac gaggaagcga tgaccatgga agtttcgccg
      841 gaaaaactga agttcgtgac gcatcgggaa tattcgtttg ggcagcagct gctggtttca
      901 tttgatgcgc ggagcgacgc gccgtgggcc ggcgatcagg agtgggaagg ccaagtgacc
      961 ggaatcgaaa tggatgccga cagcgattcg gtgaccgtga ccgtgcgcaa gaaatcggcc
     1021 tgagcgagac acgggcagat aattagctcg tgtaaactga tggctccact catgagccag
     1081 ggacatcaat cgaaaaactt cttcttagaa ggaccggcag ggaagctgga ggcggtgtta
     1141 tggacaccgc atggtgacac gccgccgaag aacacagcgc tggtctgcca tccgcacccg
     1201 ctttttggcg gaacgctgca caacaaagtc gtttaccaag cggcaaaagc attggacggg
     1261 ctcggaatgc cggtactgcg atttaatttt cgtggggcgg gtttgagcga aggcaaacat
     1321 gatcgcggac gcggcgaact ggacgatgtc cggtcggcgg tgagctttct ggcaacggag
     1381 tttccgggga ccccgctgtt gctggcgggg ttcagtttcg ggtgctgggt cggcctccgc
     1441 gttgcatgcg gagaccctcg cgtggaaagg ctgatcggca tcggcgcgcc ggtaaacagc
     1501 tcggattttt cttatttgca gcagtgcagt aaaccaaagc tattcgtgca cggttcgcat
     1561 gacgaacatg gagaagtcaa caaagtgcgg gcgatggtgg catcacttcc gggagaaaac
     1621 gacctggttg tagtcgaggg ggccgatcat ttctttaccg gaaagattga gcaactgggc
     1681 gcggcgatca gcacctgggg tgatcggggc caccaccggc atgggattta attcggtgta
     1741 gaatattcta acgtccccgc cgcaatccgg ttctcacgga ggagagccgt ccatgttcga
     1801 caaatatctt ccttactgga tgactcgtgg aatcctgcgg gccggcacag taatggccgt
     1861 gatcttgttt ttcatttcct acatcgtgga tgtaacacta gccggagtcg gaatactgcc
     1921 gtcttccacg gttttgaacg acgtggcgat tgcgctgatc gccgctgccg ttttgatgtt
     1981 ttacctgttt tccagccgca cggaacaaat ctttctgcgc gcgcgcgagc gcatgcacct
     2041 caccgccgag ttgaaccacc atatgcggcg cgtgcttagc gagatgcgca atgccgcgga
     2101 agtcgaaaac cgcgcagagc ggatgcacat gatggaccat gctattgaag aagccgatca
     2161 cctattgatc gacctggtgc cgacggtcag cgccgaacgg tcgccgcgta tcaggtcgtt
     2221 tgagaatcgg tgaaccgtca ttctcgcgaa acagaactaa ccggcctggc cacgcgagaa
     2281 cagctgagaa aactggtcga ttgtggccgg cggaatccga acgagtcttg cattcgccag
     2341 tacgtgctgc tcgcgtgaca tccctaccag ggccgtggtg atgcccggcg atgaccttac
     2401 aaattgcaga gcgcgttccg cgtccgattc cagacccaaa gcgtcggcca caaaagaagg
     2461 caaattgctc acgacctggc cctgaagaag agatgcgctg gcaatcaggg taatgttcag
     2521 ctcttcagcc gcttccataa tggtaagcgt ttttccagcg accgactgat taccgagcgt
     2581 cagcgcttcg gtcatgccga gattgaacgg cagttgcacg aagcgaaagt gatggccgtc
     2641 gcccgcgatc tctttcgcaa tggcgacaaa atcagaaagc tgcatggcgt cgcgcacggc
     2701 cgcctcctgg cgaaaaccgt tccaggtggc cattccatag aactgtattt tcccgctgcg
     2761 gacagtcgcc tccagaaaat gaaacgcgtc gcggacgcgc gaaagaaact gcggcttggg
     2821 gatttcggtc agctgggttt cgggattgtg gagatagaag acgtcaatgg aatccacgcc
     2881 catgttggcc aggctgcgcg ccagttgatc ctgcaagaaa cgcggcgcca tgcaatggct
     2941 gcccgcggaa atgtccttgg cctgaaaaat tccgcgctga atgtattctt caaaaaaata
     3001 acgattgggg tcggctggca atttggcgtc gggcgtcagg taaccggcct tggtgcacac
     3061 cacgatttgt tcgcgcacat atcctttgtc ttcgagttgc gccaacgccg cgccgacgct
     3121 gcgttcgctg cgttgaaacc ggtaattaat ggcggcgtcc acgacgttga tgccgttttc
     3181 gacggcggcg acaatcgcgg acgtgtagcc cgcgtcggtg cgttcatcgg gctggccgag
     3241 gtaggtgcca atgccgagcg aggataaaac cagcccctgc gtttcccgga agtggccgtc
     3301 cgcggcgtgc gcatggaagc gctccgcata agctcgcgtg ccatcatttg tcgctttctc
     3361 gctcatcacc ggtcctattt tcgcaagatc gaaaactgcg aaagtatagc agacggattc
     3421 gcggcgctgc gaaaagctac cgcaaacgac atttccagtc aggacacagc gcggcggaga
     3481 aggtccagct gttcaagcgt agctcagtat catgaatgtg gaaccgagat gccgccttcc
     3541 atcgtgga
//
LOCUS       DASTPM010000107         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_810793, whole genome shotgun sequence.
ACCESSION   DASTPM010000107 DASTPM010000000
VERSION     DASTPM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810793"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFR42_01865"
     CDS             complement(<1..326)
                     /locus_tag="VFR42_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0367925.1"
                     /translation="MPQQFGLVINGNPHTIQADPDTNLLTVLRDTLGLTGSKYGCGEG
                     QCGACTVLLDGAARRACITPVSAAAQKLITTIEGLEQAGRLHPVQQAFVNNEAFQCAY
                     CTSGMI"
     gene            complement(418..2007)
                     /locus_tag="VFR42_01870"
     CDS             complement(418..2007)
                     /locus_tag="VFR42_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acido-empty-quinoprotein group A"
                     /protein_id="HEU0367926.1"
                     /translation="MKFSLAKFLRLMLTPVLLSSGLLPFRLAAQGLDPAALLNPPTDT
                     WPTYNGDYSGRRHSTLDQINASNVSSLTLAWAFRIRGSAIKSTPLVVNGVLYFTTPDN
                     VWAVDARYGHQVWHYHRASEGDHIGQRGLAMYKDYLFFETPDAHLICLNAKDGTVRWD
                     VQLADSKLGYFSTMAPLVVRDHVISGVSGDVTDIPGFLVSIDPETGAVQWRWNTQPKP
                     GEPGSETWPKDSDAIRHGGGMTWMTGTYDPALNLLYWGTGNPNPVLAGEGRPGDNLFT
                     CTIVALNPDTGKLVWYFQPSPHDVHDWDAVQTPVLFDADFKGKPRKLVAQASRNGFFF
                     VLDRETGEHLVTEPFIEQTWASGVDKRGRPISKPDSTPAPDGVLVSPGSDGATNWMAP
                     SFDPQSGLFFVNARRLFSVYYNTVTTKAEGWGGRDRILWANSAIRALDYRTGKVVWNR
                     EIGDGESIAGILTTAGRLLFTADNSGNLLALNPANGETLWHLNMGGALVASPMTYQLD
                     GRQFVILPVDNVLYALALPQK"
     gene            complement(2035..>2820)
                     /locus_tag="VFR42_01875"
     CDS             complement(2035..>2820)
                     /locus_tag="VFR42_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0367927.1"
                     /translation="RNLSAFLVLCAVFLLASIDLRSQAQAPPQPTPEQRAAIDRGRTQ
                     FQQACGFCHGPDATGARGPDLVRSALVAHDVNGDLIGEVIRDGRPDKGMPALPLTAAQ
                     ISDISAFLHARSKEAVESSGVPSDYPFEKLLTGDADKGKAYFEGAGGCSKCHSPAGDL
                     AGIAHKYNAIELQARMLYPEVKPVLATVILPTGEQLSGIVSHIDDFSVGIRIGDKNGW
                     YRSFPRSRVKLELKDPLAAHRELLPKISQTQMHDLFTYLYSLK"
BASE COUNT          606 a          712 c          947 g          555 t
ORIGIN      
        1 atgatcatgc ccgaagtgca gtaggcgcat tggaaggctt cgttgttaac gaaggcttgc
       61 tggacgggat gtagacggcc agcttgttcg agtccctcga tggtggtgat gagcttttgg
      121 gcggcggcag aaacgggagt aatgcaggcg cggcgagcgg cgccgtcgag cagcacggtg
      181 caggcgccgc attgcccttc tccgcagccg tacttggatc cggtaaggcc tagagtgtcg
      241 cgaagaacgg tgaggaggtt cgtatcggga tcggcctgga tagtatgagg attgccatta
      301 atgacgagac caaactgctg cggcatttcc acccgccaaa gccgcctgcc gagagacagc
      361 ggccgctacg tgaatcgcaa caccaacatc gtcatactgt gcgacgcgag atgcaaatta
      421 tttttgcggc agggccaaag cgtatagcac attgtcgaca ggaagaatta cgaactggcg
      481 gccgtcgagc tgatacgtca tcggagaggc cacgagcgct ccgcccatat tgaggtgcca
      541 gagcgtttcg ccgttggcgg gattgagagc gagcaggttg ccggaattat cggcggtaaa
      601 gagcaggcgg ccggcggtgg tgagaatgcc ggcgatgctt tcgccgtcgc caatttcgcg
      661 attccacacg actttaccgg tgcggtaatc gagagcacgg atggcggagt tggcccacag
      721 gatgcggtcg cggccgcccc agccttcggc cttggtagtg accgtgttgt agtaaacgct
      781 gaagaggcgg cgcgcgttga cgaagaaaag accgctctgc ggatcgaagc tgggagccat
      841 ccagtttgtg gcgccgtcag agccgggaga gacgagcacg ccgtcgggag caggcgtcga
      901 atcgggtttg ctgatcgggc ggccgcgttt gtccacaccg gacgcccagg tttgttcgat
      961 gaacggctcg gtgaccagat gttcgccagt ttcgcgatcg aggacaaaga aaaatccgtt
     1021 gcggctggcc tgggccacaa gtttgcgagg tttgcccttg aagtctgcgt cgaagagcac
     1081 aggagtttgt acggcgtccc agtcgtgaac gtcgtgcgga gaaggctgga aataccagac
     1141 gagttttccg gtgtcgggat tcagcgcgac gatggtgcac gtaaagagat tgtcgccggg
     1201 acggccttcg ccggccagta cgggattcgg attgccggtt ccccaatata aaaggttcag
     1261 tgcgggatcg tacgtgccag tcatccaggt cataccgccg ccatggcgga tggcgtcgga
     1321 atcttttggc caagtttcgg aaccgggctc gccgggcttg ggttgtgtgt tccagcgcca
     1381 ctgcacagcg ccggtttcgg gatcaatcga aacaagaaag cccggaatgt ccgttacgtc
     1441 tccggaaact cctgagatga catgatcgcg aaccaccaag ggagccatgg ttgaaaagta
     1501 gccgagtttg ctgtcagcca gctggacatc ccaacgaaca gtgccgtcct tggcgttgag
     1561 acagatgaga tgagcgtcgg gcgtttcgaa aaagagatag tccttataca tggcaaggcc
     1621 gcgctggccg atgtggtccc cttcggaggc gcgatgatag tgccaaacct ggtggccgta
     1681 acgagcatcg acggcccaga cgttgtcggg cgtggtgaaa tagagaacgc cgtttacgac
     1741 cagcggagtg gacttgatgg cgctgccgcg aatgcggaag gcccaggcga gggtaagcga
     1801 gctgacgttg gaagcgttga tctggtcgag cgtgctgtgg cggcggccag agtagtcgcc
     1861 attgtaggtg ggccaggtgt cggtcggagg attgagaagc gcagcaggat ctaggccctg
     1921 agcggccagg cggaatggaa ggagccctga agacagcagc actggcgtaa gcataagccg
     1981 tagaaatttt gcgagcgaaa acttcatgcg tgatggctcc atttttgcgc gaagctactt
     2041 caagctgtat agataagtga acagatcgtg catttgggtt tgcgagattt tgggaagcaa
     2101 ttcgcgatgc gctgcgagtg gatctttaag ttcgagtttg accctgctgc gcgggaaaga
     2161 gcgataccaa ccattcttgt caccgatgcg gatgcccacg gagaaatcgt cgatgtggga
     2221 gacgattccg gagagctgct cgccggtggg cagaatgacg gtggccagga ccggcttgac
     2281 ttcgggatag agcatgcgcg cctgaagctc aatggcattg tatttgtgcg cgatgccggc
     2341 tagatcgccc gctggcgaat ggcacttgct gcagcctcct gcgccttcga aataggcttt
     2401 gcctttatca gcatcgccgg tcagcagttt ctcgaacgga tagtcggagg gaacgccgga
     2461 cgattcgacg gcttctttcg accgggcgtg caaaaacgcg gatatgtctg agatctgtgc
     2521 agcggtcaaa ggcaatgcgg gcatgccctt gtccggccgg ccgtcacgga tgacttcgcc
     2581 gatgaggtca ccgttgacgt catgcgccac cagcgcggaa cgcaccagat cgggaccacg
     2641 ggcgcccgtg gcgtccggtc catgacaaaa accgcaagcc tgctgaaatt gcgtacggcc
     2701 gcgatcaatg gcggcgcgtt gttcgggagt aggctgtggc ggggcctgcg cctgagaacg
     2761 aaggtcgatg gaagcgagca ggaatactgc acaaagaacg aggaaagctg acagatttct
//
LOCUS       DASTPM010000108         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_234173, whole genome shotgun sequence.
ACCESSION   DASTPM010000108 DASTPM010000000
VERSION     DASTPM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234173"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..2114)
                     /locus_tag="VFR42_01880"
     CDS             complement(318..2114)
                     /locus_tag="VFR42_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0367928.1"
                     /translation="MATTAIPKSRISGGSFLLEERQPSEVFTPEDFTDQHLLIGQTTE
                     EFAVNEILPNVERIEHKEFQVTRDLLKKAGDLGLSGVEIPEAYGGLEMDKVTAAVIAD
                     HIAKYAGFATTWGGHSGIGTLPIVYFGTEEQKQKYLPKLASGEMVGAYALSESTSGSD
                     AMNCRARAELSSDGKHYILNGEKMWITNAGFADLFTVFAKIDGEKFSAFLVERGYAGF
                     SVGAEEHKMGIRGSSTCPIILNDCKVPVENLLGEIGKGHIIAFNILNIGRFKLGAMCV
                     GGARVSLENAIGYAKQRKAFNKVIADFGLIREKIANMATLLFVGESLVYRTVGMMDAA
                     LSEVDKSSADALKETRKAIEEYAVECSIIKVWGSEMIDYVVDETMQIYAGYGFVEEYP
                     AERAYRDARINRIFEGTNEINRLIITGFLLKRALSGQLPLMPAIKKLMDEVLSGPSLG
                     DEIDGPLAEERKLVTQAKKLGLFAAGSATQKYMQAIQDQQEVMGAIADMTIETYAMES
                     AVLRAQKLVEAKGEKGSEMALAMTRVYLTQAMEKIEAAAKRIIAAVAEGDMLRTQLAI
                     LRRLAKHEPFNTIELRQRIAQRVIEVGKYSLA"
     gene            2538..4142
                     /locus_tag="VFR42_01885"
     CDS             2538..4142
                     /locus_tag="VFR42_01885"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU0367929.1"
                     /translation="MEPRESAFWVNLKAKRWLYTLSILATLSVGILIGSVVSYGVKGK
                     EGQQSSDAKPLSVPSPQQMSNAFSQIAKQLEPAVVNINTESTIKNPHRQRRGARPGPN
                     DDDNGGGGGGSPVDDFFDRFFGGQGGQGGPGGGDIKERSLGSGVIVDAKGYIITNRHV
                     IEKADRIRVKLQDDPPGVQHDAKVVGSDQETDLAVIKIESEKGFPTAKIGNSDSMQVG
                     DWVLAIGSPFGLQATVTAGIVSAKGRNIVPGRQFQSFVQTDAAINPGNSGGPLVNMTG
                     EVIGINTAILTETNAYAGVGFAMPSNTVVSVYNQIIGPEHKVTRGSIGVEFAAQPVPA
                     IARVYGVQSGVTISNVVPGSPADTAGLKVGDTITAVDGKEVKNGDELVADIASRKPGS
                     KAKLSFVRNSKKEETTVTIADRSKLFAARLGEEQPGNDEQQPKESKLGLSVRPLTSDL
                     ADRLDVPAGKGVIVQDVKGGSFADDIGLNRGDVILEINKQPVNSAEDFTRIEGTLKSG
                     QDVVFLVRPRGAGRQDGTIFIAGTLP"
BASE COUNT          889 a         1294 c         1159 g          896 t
ORIGIN      
        1 acgcgccgca gggatgtcgc ctggaacgat tttccctagg agatcccgat ccgccagtgc
       61 tcctgaagat ctgcgccggc ttgggactgg atgtcgcggt cgagcgtgcg gataagacgg
      121 aactacgtgc acagattggc agtgcgaaag gtgtacttac tctgacgtcc tgatcagcag
      181 ttgtcccgaa tgggtgtggg aagggcacgg cttcagccgt gccgctcagg ctcccaaaat
      241 tgcggggttt tggcccttga gattcgacac cgaacttgga atccactgag agaccgttcc
      301 gccgtcgccc gacatgtcta cgccagcgag tacttcccaa cctcaatcac ccgctgcgca
      361 atccgctgcc gcagctcgat cgtattgaaa ggctcatgct tggccaaccg gcgcaaaatc
      421 gccaactgag tccgcagcat gtcgccctca gccaccgccg cgatgatgcg cttcgcggcc
      481 gcttcgattt tctccatcgc ttgcgtgaga tagacgcgcg tcatggccag cgccatctct
      541 gatcccttct cccctttggc ttcgaccaac ttctgtgcgc gcagcaccgc cgactccatg
      601 gcgtacgttt caatcgtcat gtcggcgatc gcgcccatca cttcctgctg atcctggatg
      661 gcctgcatat acttctgcgt cgctgatccg gccgcaaaca ggccgagttt cttggcctgc
      721 gtgaccaatt tgcgttcctc ggccagcggg ccatcaattt cgtcgcccag gctcggtccc
      781 gaaagaactt cgtccatcag cttcttgatg gcaggcatca acggcagttg tccgctcagg
      841 gcacgcttga gcaggaatcc agtgataatc agccggttga tttcgttcgt tccttcgaag
      901 atgcggttga tgcgcgcatc gcggtacgca cgttccgccg gatattcctc gacgaatcca
      961 taccccgcgt agatttgcat cgtctcatca acgacgtaat cgatcatctc cgatccccag
     1021 accttgatga tcgagcactc gaccgcgtac tcctcgatgg ccttgcgtgt ttccttgagg
     1081 gcgtcggcgg acgacttgtc cacttcgctc agcgccgcgt ccatcatgcc gaccgtacga
     1141 tagaccagcg attcccccac aaacagaagc gtcgccatgt tggcgatctt ttcgcggatt
     1201 agtccgaaat cggcgatcac cttgttaaag gccttgcgct gcttggcgta tccgatcgcg
     1261 ttttccagag agacccgcgc cccacccaca cacattgcgc ccagcttgaa gcggccgatg
     1321 ttcaggatgt tgaacgcaat gatgtgcccc ttgccgattt ctccaagcag gttttcgacg
     1381 ggaactttgc agtcattcag aatgatgggg caggtggagg acccgcgaat tcccatcttg
     1441 tgttcttctg cgccgacgga gaatccggca tacccacgct cgaccaggaa cgccgaaaat
     1501 ttctcgccat cgatcttcgc gaacaccgtg aacagatctg cgaaaccggc gttggtgatc
     1561 cacatctttt cgccattcag gatgtagtgc ttgccgtcgg acgaaagttc ggcccgggcg
     1621 cggcaattca tcgcatccga tcccgacgtc gattccgaaa gcgcgtacgc tcccaccatc
     1681 tcgcctgatg ccagcttggg cagatacttt tgcttctgct cctccgttcc gaagtagaca
     1741 atcggcagcg tcccgattcc ggagtgcccg ccccaggtcg tcgcgaaccc ggcgtacttg
     1801 gcaatgtggt cggcgatgac agccgctgtg actttgtcca tttcgaggcc gccgtacgcc
     1861 tcgggaatct cgacgcccga gagtcccaga tcgccagcct tcttcagcaa atcgcgcgtg
     1921 acttgaaatt ccttgtgctc gatgcgctcg acgttgggaa ggatctcatt caccgcaaac
     1981 tcttccgtcg tctggccgat cagtaagtgc tggtcggtga agtcctccgg cgtaaacact
     2041 tcggagggct ggcgctcttc caaaaggaac gaaccgccgg aaatcctgga cttgggtatc
     2101 gccgtggtgg ccatgctgaa aacctcgttt ttctaaaact cgcagccgag aaaaaactcg
     2161 cgccgcttac cttttcgacg agccctgggc atcctgccgt gagcaatgcc gaaaggttcc
     2221 cgcagtctcc acgatatacg cgttcgcggg tcagcggcgc aagtaccgcc agtgcttgga
     2281 tttctaacac tttcgtttgc ctgttcctgg tgtcgaatta cccgaaaatg atcgtattga
     2341 cttgttgaac gaccatcttg gatgaccgcg gtttggaaga ctcagagcag gcggacatat
     2401 ttcgcgacaa cgtgaaacgc tttcgcctca caccgcgtct aagacttaag gcctttacca
     2461 cctgccacat atctgcccag atgtgccaaa caggtataat ctagccggtc acgagattcc
     2521 cggaggaagt aaacactatg gaaccgcgcg aaagcgcttt ttgggtgaat ttgaaggcca
     2581 aacgctggct ctacaccctc agtattcttg ccactctcag cgtgggtatt ttaataggat
     2641 cggttgtctc ttacggagtg aagggcaaag aaggacaaca atcgtccgat gccaagccgc
     2701 tgagtgtccc gtctcctcag cagatgtcga acgcgttttc ccagattgcc aagcaactgg
     2761 aaccggccgt ggtcaacatc aacaccgaat cgactatcaa gaacccacac cggcagcgcc
     2821 ggggcgcgcg tcccggcccg aacgacgacg acaacggcgg aggcggtggg ggcagcccag
     2881 ttgacgattt cttcgatcgc ttctttggcg gccaaggcgg acagggtggt cctggcggcg
     2941 gcgacatcaa agagcgttcg ctaggctccg gcgtcatcgt tgatgccaag ggatacatca
     3001 tcaccaaccg ccacgtcatc gagaaggcgg atcgcatccg cgtgaaactc caggatgatc
     3061 ctccgggcgt ccaacatgat gccaaggtag tcggctccga ccaggagacc gaccttgccg
     3121 tcatcaagat cgaatccgaa aaagggttcc ccaccgcaaa gatcggaaac tccgacagca
     3181 tgcaggtcgg cgattgggtg ctcgccatcg gcagcccgtt cggccttcag gctaccgtta
     3241 ctgccggtat tgtctcggcc aagggacgca atatcgtgcc tggccgccag ttccaatcct
     3301 tcgtgcagac cgacgcggcc atcaatcccg gaaactccgg cggtccgctc gtgaacatga
     3361 cgggcgaagt catcgggatc aataccgcga tccttaccga aaccaacgcg tatgccggtg
     3421 tcggctttgc catgccatcg aacaccgtcg tcagcgtcta caaccagatc atcgggccgg
     3481 agcataaggt cacccgcgga tcgatcggtg tggagttcgc cgcgcagccg gtacctgcga
     3541 ttgcccgtgt ctacggggtg caatccggcg tcacaatttc aaacgtcgtc ccgggaagcc
     3601 cggccgatac cgccggtctg aaagtcggcg acacgatcac cgctgttgat ggcaaggaag
     3661 taaagaatgg cgacgaactg gtggccgaca ttgccagccg aaagcccggc tcgaaagcca
     3721 agttgagctt cgtgcggaac agcaagaaag aggagaccac ggtcaccatc gccgaccggt
     3781 ccaaactctt tgccgctcgc ctgggagagg aacaacccgg taacgacgag caacagccca
     3841 aggaaagcaa gctcggtctc agcgttcgtc cgcttacctc cgatctcgcc gaccgcctgg
     3901 acgttcccgc cggaaagggc gttatcgttc aggacgtaaa gggcggttcc ttcgccgatg
     3961 acatcgggct caatcggggt gacgtgatcc tcgaaatcaa caaacagccc gtaaattcag
     4021 cggaagattt cactcgtatt gaaggcacgc tgaagagcgg gcaggacgtc gtctttctcg
     4081 tccgccccag aggtgcaggc cggcaagacg gtacgatctt tattgcaggt acacttccgt
     4141 aacctgatta ttgcccaacg gttactggca ctgaggtcgc gattcaaggg atgattcagt
     4201 gccggtgacc ccgggcgtat tccttgacaa cacttggt
//
LOCUS       DASTPM010000109         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1127901, whole genome shotgun sequence.
ACCESSION   DASTPM010000109 DASTPM010000000
VERSION     DASTPM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127901"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1334
                     /locus_tag="VFR42_01890"
     CDS             240..1334
                     /locus_tag="VFR42_01890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0367930.1"
                     /translation="MAITHEPAAGKFYCGVDNGIAVLPWLIFEFLQFSLRPMMPIKPV
                     LSMLSPPGESARLSILIFHRVLSQPDPIFPGEVDVRQFDQILHWVKRWCNVLPLEEAT
                     TRLKGGTLPARAAAITFDDGYADNHDNALPILQQHGLSATFFIATGYLDGGRMWNDSV
                     IEAVRNSTADHVDLQDFELAPPPGAAQWPLGSAEARRDLIGAILGHIKYADPTRRQDM
                     ADAIAARLGGNMATNLMMSSDQVRKMRRAGMEIGAHTHTHPILARLDREVAYDEIRLN
                     KTNLEGLLNEPVKLFAYPNGRPAKDYLSESVEVVRSLGFTAAVTTAVGAADRASDPLQ
                     LPRFTPWDRTKTRFGLRLAANLMGKRPIEQ"
     gene            1331..2506
                     /locus_tag="VFR42_01895"
     CDS             1331..2506
                     /locus_tag="VFR42_01895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0367931.1"
                     /translation="MNALVEHPTRGADATTALELTFEQLQLPRDVARMQLSDPALDAA
                     LQSVLGDAACNPDGQASVIVGRRSGKVVLLAPCLEQTVGLPVRLGEKILLGQQLLSVR
                     LPPSAQNGVASETDALGLLMCLRKVAARRAIVVTDVTTNSPLYLGVLRAREADYVLTK
                     NEPDTHLFHRFEASYDEFFNRRSSKQRNQLRKKEKVFAERFGTTFEFREYRQTHEVSE
                     FLTAAKAINRKTYQYRMFGESVDDDQDSVAVVHRAAAAGCFRSFVLWHDAQPVCFVLG
                     HQRNDGTFEHRQTGFDPAFRDAAPGISTNILLLRRLYEADRPHILDFGSGDSDYKRLF
                     SNESRLTANPILLPRRGHFMLAYWLYEASAALNSAAIGCLERLGVKDWIKRRLRGAA"
     gene            2649..>3010
                     /locus_tag="VFR42_01900"
     CDS             2649..>3010
                     /locus_tag="VFR42_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367932.1"
                     /translation="MQLRADVLFEFDLTDLAADRAEIANDAIIVVDRGNFGSAATALV
                     ERDVLAGPNHEYVKELHGKGTLMAVTDGEGMVTSYAFVVFESFYKRILGESTETPIIS
                     NCVTLPAYRGQGIYPRLLR"
BASE COUNT          616 a          863 c          876 g          655 t
ORIGIN      
        1 tttcccctcc tagcgcctgg cggccggagg gcccgcatga catcgcttgt ctactatcca
       61 ttgccaacgt tttacgttct cccaccagtg tgccttccgc gccaccccat cgcttttccc
      121 gcgccaaatt cgctttgcga aagcgaacgg acgaggcgga ggaaaaaact gtatcctccg
      181 catcgcgccg gatgttccgt aggcgcaggt gtggttcggc gagaaatgtc ccccttccgg
      241 tggccatcac acatgagccc gccgccggaa agttttattg tggcgttgac aacggaattg
      301 ccgtgctacc atggctaata tttgaatttc tccagttttc cctgcgcccc atgatgccaa
      361 ttaaacccgt tctttcgatg ctgtcgccgc caggcgaaag tgcgcgtctt tcgatcctga
      421 tttttcaccg ggtgctttcc cagccggatc cgatctttcc tggcgaggtg gatgtgcgcc
      481 aattcgacca gatcctccat tgggtcaagc gttggtgtaa tgtcctgccg cttgaggagg
      541 cgacgacccg actcaaagga ggcaccttgc cggcgcgcgc cgcggccatc acgtttgatg
      601 atggttacgc ggacaaccat gataatgccc tccctattct tcagcagcac gggttgagcg
      661 cgacattttt catcgccacc ggctatctgg acggcggtcg gatgtggaac gacagcgtga
      721 tagaagcggt gcgaaacagc accgctgatc acgttgacct tcaagacttc gaactagcac
      781 cgccgccagg tgcagcgcag tggccgctcg gctccgctga agcgcgccgt gatctgatcg
      841 gcgccattct cggccacatt aaatatgctg atccgacacg gcggcaagat atggccgacg
      901 ccattgccgc acggctggga gggaacatgg caacgaactt gatgatgtct agcgatcaag
      961 tgcgtaagat gcgccgcgcc ggaatggaga ttggcgcgca tacccatacg catcccatcc
     1021 ttgcgcgtct ggaccgcgag gttgcgtacg atgaaattcg gctcaacaag accaatctcg
     1081 aaggcttgct caacgaaccg gtcaaactgt ttgcctatcc gaatggccgt cccgcgaagg
     1141 attacttgtc ggagagcgtc gaagtagtgc gcagcctggg cttcacggca gcggtcacga
     1201 cggcggtagg cgctgctgat cgagcaagcg atccccttca gttgccacgc ttcactccct
     1261 gggacagaac caagacgcga ttcggtttgc gtctggcggc gaacctaatg ggcaagcgcc
     1321 cgatcgaaca atgaacgctc tggtcgaaca cccaacgcgg ggcgcggacg cgactactgc
     1381 actcgagcta acgttcgagc agctacaact gccgcgcgat gttgcgcgca tgcaattgtc
     1441 ggatccagcg ctcgacgctg cattgcaatc ggtgcttggg gatgctgcct gcaacccgga
     1501 cggccaggcc tccgtgattg tcggtcgccg aagcgggaag gtggttctgc tggccccctg
     1561 tctcgagcaa acagtcggcc tgccggtccg gctcggcgaa aaaattctcc tgggtcagca
     1621 actcctgtct gttcgccttc cgccgtctgc gcaaaacggg gtagcttcgg aaaccgacgc
     1681 attgggactg ctgatgtgcc tgcgcaaagt tgcggcgcgg cgagcgatcg tggtgactga
     1741 tgtaacaacg aattcaccgc tctatcttgg ggtgctgcgg gcgagagaag cggattatgt
     1801 gcttaccaag aacgagccgg acacgcatct gttccatcga ttcgaggcaa gttacgacga
     1861 atttttcaat cggcgcagca gcaagcagcg gaaccagttg cgtaagaagg agaaggtatt
     1921 tgcagagaga ttcggcacta ccttcgaatt tagggaatac cggcaaacgc acgaggtgtc
     1981 ggagtttctc accgccgcca aagcgatcaa ccggaaaacc taccagtacc gaatgtttgg
     2041 cgaaagtgtc gacgatgatc aggatagtgt cgcagtcgtg catcgcgccg ccgcggccgg
     2101 atgttttcgg tcgttcgtac tgtggcatga tgcgcagccg gtttgcttcg tgcttggcca
     2161 ccagcgtaac gatggcacct tcgaacaccg tcaaaccggt ttcgatcccg cctttcgcga
     2221 tgcggcccct ggaatcagta cgaacatatt gctgctccgg cgcctttacg aggcggatcg
     2281 cccccatatt cttgatttcg ggagcggtga ttcggattac aaaaggctgt tttcgaatga
     2341 atcgcgcctc accgctaatc cgatcctgct accgcgccgc gggcacttca tgctggcata
     2401 ttggctgtac gaagcgtcgg ccgcactcaa ctccgccgcc atcggctgcc tggagcgcct
     2461 gggcgtgaaa gactggataa agcggcgcct gcgtggtgcc gcgtgacgat cgactttgat
     2521 gtagccggtc gacctaattc tttctgcaca aggcgttttc gatgatgatc aatatttgca
     2581 agcggctaaa tacggaactg cgaacgcgcg ggcctgccgg ctttgcgcgt ttcattgcac
     2641 tgcgactgat gcagcttcgc gccgatgtat tgttcgagtt cgacctgact gatttggccg
     2701 cagatcgggc cgagatcgcc aacgatgcaa tcatcgtggt agaccgtggc aatttcggta
     2761 gtgccgcaac agctttggtc gagcgcgatg tgttggctgg accgaaccac gaatatgtta
     2821 aggagctgca cggtaaaggc acgctcatgg cggtgacgga tggcgaaggc atggtcacca
     2881 gttacgcttt tgtggttttc gaatcgtttt acaagcgcat tctgggtgaa tcgaccgaaa
     2941 cgccaataat cagcaactgc gtaacactgc cggcttatcg cggacagggg atctatccgc
     3001 gactgttgcg
//
LOCUS       DASTPM010000110         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_61230, whole genome shotgun sequence.
ACCESSION   DASTPM010000110 DASTPM010000000
VERSION     DASTPM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61230"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFR42_01905"
     CDS             <1..212
                     /locus_tag="VFR42_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367933.1"
                     /translation="PSISSSLFAPGYSIAAVIANEFTEATGDLYLQSLIQLGLVLFLL
                     TFILNGLARLLMLLTASRGSGTGRA"
     gene            209..1060
                     /gene="pstA"
                     /locus_tag="VFR42_01910"
     CDS             209..1060
                     /gene="pstA"
                     /locus_tag="VFR42_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU0367934.1"
                     /translation="MNARLRLRKLVSAFMLTMTGVCAVVAVAVLFFILGYLVFHGGRS
                     INWDFFTKLPTPVGEGGGGMANAIVGSAKLLFLASLFGVPIGFFGAIYLAEFSGSKTA
                     FVVRYAADLLNGVPSIVIGIFAYALVVLPFKHFSAYAGGFALGIMMIPITLRSTEEFL
                     LGVPNTLREAAMALGASKWKTIATVIVPAAYRGILTSVLLAFARVAGETAPLLFTAFG
                     NRYWSPGFNQPIESLPHMVYIYAISPYEDWQRQAWAAGLVLLGLILIVNIVARFILAR
                     GVAVQRS"
     gene            1089..1892
                     /gene="pstB"
                     /locus_tag="VFR42_01915"
     CDS             1089..1892
                     /gene="pstB"
                     /locus_tag="VFR42_01915"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU0367935.1"
                     /translation="MTVNLTPTGVINTSRETAAPAPMRMTISKVNFFYGTKQTLFDVN
                     MSVAANRVTALIGPSGCGKSTLLRTFNRMHETVRNARLEGEILLDGQNILAQDVTQLR
                     RRIGMVFQRPNPFPKSIFDNVAYGPKINGSKDPIAEVVERSLRRAALWDEVKDHLHKS
                     AYELSGGQQQRLCIARGLAVEPEVLLLDEPCSALDPISTAKIEELLVQLKDSCTIAIV
                     THNMQQAARVSDFTAFLLVGQLVEFNPTPQLFTTPSDPRTEAYITGRFG"
     gene            1900..2565
                     /gene="phoU"
                     /locus_tag="VFR42_01920"
     CDS             1900..2565
                     /gene="phoU"
                     /locus_tag="VFR42_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU0367936.1"
                     /translation="MERHFERDLNELKERLLWMGSLAERSVHQSVHAVLDSDEQLAKR
                     VLEEESAINELQIEIDDRVVQLLALHQLMAMDLRFVLAVSRINNDLERIGDQAVNVAQ
                     GALRILRHPRVKPYVDLPRMSEVAEEMVRDSLNAVVRRDVDLARKVLESDDKVDHYRD
                     QIFRELLTYMMGDSSVVFPAFELILVAKNLERIGDHATNIAEDVIYIVQGQDVRHPAV
                     DRR"
     gene            complement(2562..>3746)
                     /locus_tag="VFR42_01925"
     CDS             complement(2562..>3746)
                     /locus_tag="VFR42_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367937.1"
                     /translation="CGFLEEQTDHSDRLQGIFFFAIAFSAVWLSNAPAAVIATYSVTF
                     LFLFAALQQRSPKPVLHGGGAMLLGFGLASFYLIPAIYEQRWVNITGALAGGLTPDEN
                     FLYATTKDAEHDAFNRVASNIAVVLLVWCGAGALAAWRTKPRSGFRLAALLPLATLTA
                     GVLLLLLPLTSLLWRFLPELRFVQFPWRWMSVLALCLMIFMAATATAKFRWIWLAVTA
                     VAAAGCGHYIAKHTWWDTEDMPNLEAAMASGEGFEGTDEYDPVGDDRTELAQKQPRAW
                     FVSEAGQAEERSDERISVDRWTAEHRALRSVTRRDARIAIRLLDYPAWQVSVNGKLMK
                     PRHGAATNQMILPVAAGESRIEIHFARTWDRTIGGWVSVLTACGLMGLGLWSRKRTDA
                     PL"
BASE COUNT          772 a         1205 c          979 g          790 t
ORIGIN      
        1 ctccgtctat tagcagctct cttttcgctc ccggatattc cattgcggct gtgattgcca
       61 acgaatttac ggaagccacg ggagacctgt acctccagtc gctgattcaa ctagggctgg
      121 tgctcttcct gctcaccttc atattgaacg gtctggcccg gctgctcatg ctgctcaccg
      181 cctcgcgcgg cagcggaacg gggcgcgcat gaacgcacgt ctgcggttgc gaaagctcgt
      241 cagcgcgttc atgctcacta tgaccggcgt gtgcgcggtt gtcgccgtcg cggtcctctt
      301 tttcattctt ggctacctgg tattccatgg cggccgctcg attaactggg acttcttcac
      361 caagctgccc actccagtag gtgaaggcgg cggcggcatg gccaacgcca tcgttggcag
      421 cgcgaaactg ctcttcttgg ccagcctgtt tggtgttcca atcggtttct ttggagcgat
      481 ctatctcgcc gagtttagcg gttcaaagac cgcctttgta gttcgctatg ccgccgacct
      541 gctgaacggc gttccctcca tcgtcatcgg catcttcgcc tatgccctgg ttgtcttgcc
      601 cttcaagcat ttctcggctt acgccggagg cttcgctctg ggcatcatga tgatccccat
      661 tacgctgcgc agcacggaag aatttctgct gggagtcccg aacacgctcc gcgaagcggc
      721 catggccctt ggggccagca aatggaagac gatcgccacc gtgatcgtcc cagccgccta
      781 ccgcggaatt ctcacctctg tcctcttggc ctttgcccgc gtggccggcg aaacggcccc
      841 gctacttttc accgcgttcg gcaatcgtta ttggagccct ggcttcaatc aacccatcga
      901 gtccttgcct catatggtct atatttacgc catttccccc tacgaagatt ggcaacgtca
      961 ggcatgggcc gctggcttgg tgctgctcgg gctgattctg attgtgaata tcgtcgctcg
     1021 gtttattctg gcccgaggcg tagcagtaca gaggtcttaa ccatatgcca ggttcagcga
     1081 ggtctgcagt gaccgtgaat ttaactccca ccggcgtcat aaacaccagc cgcgaaactg
     1141 ctgcgccagc acccatgcgc atgaccatct ccaaggtcaa tttcttctac ggcaccaagc
     1201 aaaccctttt cgacgtaaat atgagcgtcg ccgccaatcg cgtcaccgcg ctcatcggtc
     1261 cttcgggttg cggcaaatcc actttgctgc gcaccttcaa ccgcatgcac gaaaccgttc
     1321 gcaatgcgcg cctcgaaggt gaaattctct tggatgggca gaacattctt gcgcaagacg
     1381 tcactcaatt gcgccgccgg attggcatgg tctttcagcg ccctaatcct ttcccgaaat
     1441 cgattttcga caacgtggcc tacgggccga aaatcaacgg atcgaaggac ccgattgcgg
     1501 aagtcgtcga gcgcagcctt cgccgtgccg ccctatggga cgaagtcaag gaccacctgc
     1561 acaaatcggc ctatgagctt tccggcggcc agcaacagcg gctctgcatc gcccgcggcc
     1621 tggctgtgga accggaagtc ctgctcctcg acgaaccctg ctccgcgctc gaccccatca
     1681 gcacggcaaa aatcgaagag ctcctcgtgc agctgaaaga ctcctgcacc atcgccatcg
     1741 tgacccacaa tatgcagcaa gcggctcgcg tcagcgactt tacggcgttc ctgctggtcg
     1801 gccaattggt ggaattcaat ccgacgccgc aacttttcac cacgccttcc gatccgcgca
     1861 cagaggcgta catcaccggc cgtttcggat gaggaatcta tggaacgtca cttcgaacgc
     1921 gatctgaatg aactgaaaga acgcctgctg tggatgggca gcctcgcgga gcgctccgtg
     1981 caccagtcgg tccacgccgt gctggactcc gacgagcagc ttgccaagcg cgtcctcgaa
     2041 gaagaaagcg ccatcaacga gctgcagatc gaaattgatg atcgcgtcgt tcagcttctc
     2101 gctctgcacc agctgatggc catggactta cgtttcgtcc tggccgtttc gcgcatcaac
     2161 aatgacctgg aacgcatcgg cgatcaggcc gtcaatgtcg cccaaggggc gctgcgcatc
     2221 ctgcgtcatc cccgcgtgaa gccctacgta gaccttccgc gcatgagcga agtcgccgaa
     2281 gaaatggtgc gcgactcgct gaacgctgtt gtccgccgcg acgtggatct ggcccgcaaa
     2341 gttcttgaaa gcgacgacaa agtcgaccac taccgcgatc agatttttcg cgagcttctg
     2401 acttacatga tgggcgattc cagcgtggtc ttcccggcgt tcgagctcat cctggtcgcc
     2461 aaaaatctcg aacgcattgg cgaccatgcc accaacatcg cagaagacgt catctacatc
     2521 gtgcaaggcc aggacgtgcg ccatccggca gtcgatcgcc gctagagcgg cgcgtccgtc
     2581 cgttttctgc tccacaaccc aagacccatc aagccgcacg cggttaaaac cgaaacccat
     2641 ccgccgatgg ttcgatccca cgttcgcgca aaatgaattt cgattcgcga ttcgcccgcg
     2701 gctaccggga gaatcatctg gtttgttgct gctccgtggc gcggcttcat caactttccg
     2761 ttcacggaaa cctgccaagc cggatagtcc aatagtcgga tcgcaatgcg tgcgtcgcgg
     2821 cgtgtcaccg agcgcagagc gcgatgttcc gcggtccagc gatccacaga aattcgttcg
     2881 tcgctccgct cctccgcttg accggcttcc gaaacaaacc acgcgcgcgg ctgtttttgg
     2941 gccagttcgg tgcggtcatc gcccacagga tcgtactcat ccgtaccctc gaatccctcg
     3001 ccgctcgcca tcgctgcttc caggtttggc atgtcctccg tgtcccacca ggtgtgcttt
     3061 gcgatgtagt gtccgcaacc tgcagcagcc actgcggtca cggccagcca gatccagcga
     3121 aactttgcag tggccgttgc cgccatgaag atcatcagac agagcgccag cactgacatc
     3181 caccgccacg gaaactgcac aaaacgcagt tccggcagaa atcgccaaag gagtgacgtt
     3241 agaggcagca acaacaacaa cacgccggcc gtcagcgttg cgagcggcag cagcgctgcc
     3301 aatctgaacc cgctgcgcgg tttggtgcgc cacgcggcca gtgcacccgc gccgcaccac
     3361 accaacaaca ccaccgcaat gttggatgcc acgcgattga atgcatcgtg ttccgcgtct
     3421 ttggtcgtcg cataaagaaa attctcatca ggggttaatc cgcccgccag cgcgcccgtg
     3481 atattcaccc agcgttgttc gtaaattgcg ggaatcaggt aaaagcttgc caaaccaaag
     3541 cccagcaaca tcgcgccgcc cccatgcaac accggcttcg gtgaacgttg ctgcaacgcg
     3601 gcaaatagaa agagaaacgt aaccgaatac gtcgcaatca ccgccgcggg tgcattcgaa
     3661 agccacactg cactaaacgc aatcgcaaaa aaaaatatcc cctggagccg gtctgaatgg
     3721 tctgtctgtt cctcaaggaa gccaca
//
LOCUS       DASTPM010000111         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1301058, whole genome shotgun sequence.
ACCESSION   DASTPM010000111 DASTPM010000000
VERSION     DASTPM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301058"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFR42_01930"
     CDS             complement(<1..719)
                     /locus_tag="VFR42_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367938.1"
                     /translation="MTTVTVDGSEAMFTVMCALLASGFESDVNATSWSPVRAQLRDLL
                     QRQQGPAVDVMRTFFKQHELSDAGAMLSRYIWFGLVSGPAPKFEATMRRDELPPEVIA
                     LEGFQEILSNYYKEQKIGELWRQVQSVHAREIEQMHDALSQVVFVATGYLRQILDPTN
                     PRTFTVIVEPLVGRITNVRNFGDHYALVVGGAEQLQLDTVRHAFLHYLLDPLPLQYSH
                     VIAVKRPLFEMAAAAPRLDPSL"
     gene            1024..2241
                     /locus_tag="VFR42_01935"
     CDS             1024..2241
                     /locus_tag="VFR42_01935"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEU0367939.1"
                     /translation="MRKTTLRFLFSIFALTGVAFISSPARAQLVGDTPPQQLTNSPAA
                     DQPTQSTAKPTGKKRISKDFTLQGDSLWTDTGIDLQPGERVVITAKGTVRYADAKSDN
                     GPDGLTRGFKDLLRVLPFNDAGRGALIARIGDADTAPAFLIGVSKDTISPIVGRLALG
                     INQAKSETADGTYTVHVDVYATTGGAVRTVSKVVETMPGIDHDLFAKIPRRIADKEGN
                     PGDMVNFLILGTEAAMQKVFTTAGWVKVDADVKGTVLHGLIGSLSKESYLTMPMSPLY
                     LFGRQQDCGWAHAEPISVVASRNHLRIWKAPFTVNGTMVWVGAATHDVGFERDQRNNG
                     VTHKIDPDIDLERDYVQKTLSSTGLVVEISHFLPENPMKEARTATGGSFHSNGQVLIL
                     RLADSTKDLAAVN"
     gene            complement(2341..>2865)
                     /locus_tag="VFR42_01940"
     CDS             complement(2341..>2865)
                     /locus_tag="VFR42_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367940.1"
                     /translation="HQEGSPTHGSWFTATSMANLMKVLEGLRNAPGATAPVLAASKHW
                     DFILRSTEHNGQAGTFTNGYLRVARWPAKAGGNDPDGKIQRATMVGILEKLFADGALH
                     SYTIDEEVVHSEDPGTTYVVFVANGAEGLDKFDAALAEIRKSNPVALAAYGTLIESHG
                     HRDTLARVNTMTDK"
BASE COUNT          586 a          920 c          749 g          610 t
ORIGIN      
        1 agcgacgggt ccaggcgcgg tgccgccgcc gccatttcga acagcggccg ctttactgcg
       61 atgacgtggg aatactgcaa cggcaaaggg tccagcaaat aatgtaaaaa cgcgtgtcgc
      121 accgtatcca gttgaagctg ttcggcgccg cctaccacca gcgcgtagtg atcgccgaag
      181 ttcctcacgt tcgtgatgcg ccccaccagc ggctccacga tcaccgtgaa cgtgcgcgga
      241 ttcgtggggt ccaggatctg ccgcaaatat cctgtcgcca caaacaccac ttgcgacagc
      301 gcgtcgtgca tctgctcaat ttcccgcgca tgcaccgatt gcacttgccg ccatagttcg
      361 ccaatttttt gttccttgta ataattcgaa aggatttcct ggaagccttc tagagcgatc
      421 acttcaggag gcaattcatc acgccgcatc gtcgcttcga atttcggcgc tggcccgctc
      481 accagcccga accagatgta ccgagacagc attgctcccg cgtccgagag ttcgtgttgt
      541 ttgaaaaacg tccgcatcac atcgaccgcg ggaccttgct gccgctggag gaggtcgcgc
      601 agttgcgcgc gcactggcga ccaacttgtg gcatttacgt ccgattcgaa gccggaagcc
      661 agcaacgcgc acatcaccgt gaacatggcc tcgctgccgt ctaccgtaac tgtggtcacg
      721 ggtgccgtct gtggccggcg ttgcggcagc ggctcctgct tttgagcagc cccaggtaag
      781 gccaacgccg cactcaacac tattgccaga atccagtagg ttattttcat cccattgcgg
      841 gccgctgcgg acgagcccgt ccttcgaatc gttctgttca agtgccgttt ttcttcgtcg
      901 tttcaatcta caagcgtgcc gaaggccagt caagaaaccg ccggctaccg caccggcaac
      961 tcttgccatt actcgatgca tctgcctaaa ttgtggatgc ttgctagtat tgctggcggt
     1021 tcaatgcgaa agaccaccct gcggtttctc ttctcgattt ttgctttgac cggtgttgcg
     1081 ttcatctcta gcccagctcg cgcccaactg gttggcgaca cgcctccgca gcagctcacc
     1141 aattctcccg cggccgacca acccacccaa tccactgcca agcccaccgg aaagaagcgc
     1201 atctcaaagg atttcaccct tcaaggcgac tccctctgga ccgacaccgg catcgacctg
     1261 cagcccggtg agcgcgtggt catcacggca aagggcacgg ttcgctacgc tgacgccaaa
     1321 agcgacaatg gcccagacgg cctcacacgc ggtttcaagg accttcttcg cgtcctcccg
     1381 ttcaacgacg ccgggcgagg tgctttgatt gcccgcatcg gcgacgccga caccgcaccg
     1441 gcctttctca ttggtgtgtc caaagacacc atttcgccca tcgttggacg cctggcgctc
     1501 gggatcaatc aagccaaatc cgaaacggcc gacgggacgt acaccgttca cgtggacgtg
     1561 tacgcgacca ctggtggcgc cgtccgaacc gtctcgaaag ttgtggaaac tatgccgggc
     1621 atcgaccacg atctcttcgc caaaatcccc cgtcgtatcg ccgacaagga aggcaatccg
     1681 ggcgacatgg tcaacttcct catcctgggc accgaagccg ccatgcaaaa agttttcacg
     1741 actgcaggct gggtcaaagt cgacgccgac gtcaaaggca cagttctgca cggacttatc
     1801 gggagcctct ctaaggagtc ctatctcacc atgccgatga gcccgctgta tctttttggc
     1861 cgccagcaag attgcggctg ggctcacgcc gagcccatta gtgtggtcgc gtcgcgcaac
     1921 catcttcgca tttggaaagc tccgttcacc gtcaacggca cgatggtctg ggtcggcgcg
     1981 gcgacgcacg atgtcgggtt cgaacgcgat cagcgcaaca acggtgtcac gcacaaaatc
     2041 gatcccgata tcgatctgga gcgcgactac gtgcaaaaga ctttgtcgag cactggtctc
     2101 gtggtggaaa tctctcactt ccttcctgag aatccaatga aggaagcgag gaccgcaacc
     2161 ggcggttcct tccattcaaa cggccaagtg ctgattctca gactcgctga cagcaccaag
     2221 gacctcgctg cggtcaacta attcggggcc gcctgcggca cttgatccac aaaaaaaagc
     2281 cctggactaa cgtggtcttg ctcgccgtct cgaggccgcc gacattgctt ctggtgtttc
     2341 ctacttgtcg gtcatcgtat ttacgcgagc cagtgtgtcg cgatggccgt gtgactcgat
     2401 caacgttccg tacgcagcca gcgctactgg attgctcttc cgaatttcag cgagggccgc
     2461 atcaaactta tccagccctt cggccccgtt cgccacaaac accacgtacg tcgtgccggg
     2521 atcctcagag tgaacgacct cttcatcgat tgtgtacgaa tgcaaggctc catccgcgaa
     2581 cagcttttcc aaaatgccta ccatcgtggc tcgttggatt tttccatcgg ggtcgttgcc
     2641 gcccgctttt gccggccagc gcgccacgcg caaataacca ttcgtgaacg tgcccgcttg
     2701 tccgttgtgc tctgtgctgc gcagaatgaa gtcccagtgt ttcgacgcag caagaaccgg
     2761 cgccgtcgct ccgggagcat tccgcagacc ctccagcacc ttcatcaaat ttgccatcga
     2821 agtcgccgta aaccacgacc cgtgagtcgg cgagccttcc tgatg
//
LOCUS       DASTPM010000112         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_638068, whole genome shotgun sequence.
ACCESSION   DASTPM010000112 DASTPM010000000
VERSION     DASTPM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638068"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /gene="galE"
                     /locus_tag="VFR42_01945"
     CDS             <1..694
                     /gene="galE"
                     /locus_tag="VFR42_01945"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU0367941.1"
                     /translation="YFRNNTAATLTLLEACLRHKITRFVFSSSCSVFGFPQTVPISED
                     APKLPVNPYGQSKLQTEIMLEWFRQIHGLRYAVLRYFNAAGAWDGHAERHDPESHLIP
                     NLLRSALGSSGPVRIFGTDYPTPDGTCVRDYIHIYDLAQAHLLVLRALEDSGQRIYNL
                     GNGTGFSIREVLAAARRVTGREVLFIESPRRPGDPPALVASSEKIRRELHWVPEYPHL
                     DDILSSAWLALK"
     gene            complement(724..2181)
                     /locus_tag="VFR42_01950"
     CDS             complement(724..2181)
                     /locus_tag="VFR42_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0367942.1"
                     /translation="MPLAIPLSKGGGPLSRQVYARLRHAILSGALRSGEKLPSTRDLA
                     EQLAISRTVVLLAYDQLLAEGFVVGRQGSGTYVSSAVSASVSAASRNSAALRLSRFGS
                     AAAAAWARVNYPPRPERPLPYDFAYGISDVENFPFEMWRRILTRCARRAPVSELDYGP
                     AGGNAALRDAICAHLRRSRAVVCDASQVIVVNGSQQALDLIGRVLTEPGDRVALEDPS
                     YQGTREVFRAAGARVIPVPVDRLGLDPLKLPLDARLTVVTPSHQFPTGVILPLARRLA
                     LLQWAKRSNAIVIEDDYDGEFRYDGQSLDSLQSLDHEGRVIYIGTFSRTVFSTLRIGY
                     LIAPKRLVRAFLAAKWLCDRQTATLEQETLAEFISSGLYERYLRRVRRRNAARRDALL
                     GAVQAYWGDRVEVTGHEAGGHVVLWPRDPISEASIIKRAAAQGVRVYGISGYCLRKSP
                     RSGIILRYSRMNEKEIREGIRRLSEAVSTRSIRSA"
BASE COUNT          505 a          803 c          687 g          472 t
ORIGIN      
        1 ctatttccgc aacaataccg ctgccacgct cacacttctc gaagcctgtc ttcgccacaa
       61 aatcacgcgt tttgtcttct cctcctcctg ctccgtcttc ggttttccgc aaacggttcc
      121 catcagcgaa gacgctccca aactccccgt caatccctac ggccagtcca aactgcaaac
      181 cgaaatcatg ctcgaatggt ttcgccagat tcacggactc cgctacgccg tcctccgtta
      241 tttcaacgcc gccggcgcgt gggatggcca cgccgaacgc catgaccccg agtcccatct
      301 catccccaac ctgctccgct ccgctcttgg ttcgtccggt ccagttcgca tttttggcac
      361 cgactatccc actcctgacg gcacttgtgt ccgtgactat atccacatct acgacctcgc
      421 ccaggcccac ctcctcgtcc ttcgtgctct tgaagactct ggtcagcgta tctacaatct
      481 cggcaacggc accggcttca gcatccgcga agtcctcgct gccgcccgcc gagtcaccgg
      541 ccgcgaagtt ctcttcatcg aatccccccg ccgccccggc gatccccccg ccctggtcgc
      601 cagctccgaa aaaattcgcc gcgaactcca ctgggttccc gagtatccgc acctcgacga
      661 cattctttcc tccgcttggc tcgccctgaa gtgagaacgc aaaagcgtga cgattggaaa
      721 atttcaagcg cttcggatac tcctggtgga caccgcttcg ctcaaccggc ggatgccctc
      781 gcggatttct ttttcgttca tgcgcgaata acgcaggatg attccgctgc gagggctctt
      841 cctgaggcag taaccggaga ttccatacac ccgaacgccc tgcgcagcgg cccgcttgat
      901 gatggacgct tcgctgatgg gatcgcgtgg ccacagaacg acatgccctc cggcctcgtg
      961 acccgtcact tcgacgcgat ctccccaata ggcttggacg gcccccagca gcgcatcacg
     1021 acgcgctgca tttctgcgcc gcacgcggcg caggtagcgt tcatacagtc cgctggagat
     1081 aaattcggcg agagtctcct gttcgagagt cgctgtctgt cgatcgcaaa gccacttggc
     1141 ggccaggaat gcgcgaacca ggcgcttggg tgcgatcagg tagccaatgc gcagcgtgga
     1201 aaaaacggtg cgcgaaaaag ttccgatgta aatcacccgg ccttcgtggt ccaggctttg
     1261 cagcgagtcg agcgattgac cgtcatagcg aaactcgccg tcatagtcat cctcgatgac
     1321 gatcgcgttg ctgcgcttgg cccattgcaa gagcgcgaga cgcctggcca gcgggagaat
     1381 cacaccggtt ggaaactgat gcgacggcgt taccacggta agccgcgcgt ccagtggcag
     1441 cttcagagga tccagtccca gtcggtccac gggcacggga ataacgcgag cgccagcagc
     1501 gcgaaacact tctcgcgtac cttgatagct cggatcttca agagccaccc gatcgccggg
     1561 ttcagtcagg acgcggccga tcagatcgag cgcttgctgc gaaccattta ctacgatgac
     1621 ttgtgaggcg tcacagacga cggcgcgcga gcggcgcaag tgcgcgcaaa tggcgtcgcg
     1681 caaagcggcg ttaccgcccg ccggaccgta gtcgagttcg gagacaggcg ctctgcgcgc
     1741 acaccgggta agaatgcggc gccacatttc aaacgggaaa ttctccacgt cgctgatgcc
     1801 gtacgcgaag tcatacggaa gtgggcgctc cggccgtggt ggatagttga cccgggccca
     1861 cgcggcggcg gctgccgaac cgaaacgtga cagccgcagc gcggcagaat ttctggaggc
     1921 agcggacacg ctggcgctta cggcgctgga aacatacgtt cccgaaccct ggcggcccac
     1981 gacaaaccct tcggccaaca actggtcgta ggccagcagt accaccgtcc tggaaatcgc
     2041 aagttgctcg gcgagatctc tggtggatgg aagcttttcg ccgcttcgaa gggcgcccga
     2101 taaaatcgcg tgacggagcc gcgcgtatac ctggcgggac agtggtccac cgcccttcga
     2161 aagtggtatt gccaatggca caagaagtgg tgcttgcaag ataccacaag ccgcttcaag
     2221 gtcgagggct gcggaaacaa gaacggtttg ccaacggaga gtgaccgatg cacgagcggc
     2281 taagcagtcg aggatgtttt ttgatgtggg ttatgctgtt tgcgatgggt acttgcgccc
     2341 cgctgccagc aagagcgaac gccgctccgc ccaacggcca gggcgcaaaa gccggcaccg
     2401 aacacgacgg cagccatgac ttcgacttcg agattggcac ttggaaaact cacctcaagc
     2461 gtttggc
//
LOCUS       DASTPM010000113         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_897608, whole genome shotgun sequence.
ACCESSION   DASTPM010000113 DASTPM010000000
VERSION     DASTPM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897608"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFR42_01955"
     CDS             <1..732
                     /locus_tag="VFR42_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU0367943.1"
                     /translation="DDCGRSCRCFGTDSSPICALTMTTRLATLPVKHPSAALLFVQIM
                     SILLYPLMGDKSAERALFGVIGLLVLGAALYVVKRGPWLTGFAVMLALPVVGLSIWLA
                     FHFDPSRMVIMAALEAVFYFYATGSLIAYMLEDEFATVDELFAAGATFTLLAWAFAYV
                     YVVCQGLLPGSFGALTGPGNQRTWMELLFLSFTTLSGVGLGDIVPLLPMARGLVMIEE
                     FSGVMYLALVVSRLIALSVKTRTAR"
     gene            781..1515
                     /locus_tag="VFR42_01960"
     CDS             781..1515
                     /locus_tag="VFR42_01960"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0367944.1"
                     /translation="MDAFFTSSGLVALITLTFLEIVLGVDNVIFISILASKLPRSQQD
                     LARRMGLLAAMGMRILLLLSIVWITRLTAPLFHVLERPISGRDLILIAGGLFLLAKAT
                     IEIHDRLEGEEGHSSARVRPSFAAVIAQIVVLDIVFSLDSVITAVGMANEITVMVTAV
                     ILAVGVMMFSAGPISTFVNRHPTVKVLALSFLLLIGVALVGDGLGMHIPKGYIYFAMG
                     FSVFVEMINLRVRRATAPVHLHEPYI"
     gene            complement(1583..>2045)
                     /locus_tag="VFR42_01965"
     CDS             complement(1583..>2045)
                     /locus_tag="VFR42_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367945.1"
                     /translation="FMWAALSAQRKNPFVDVKLDFILGNAMAGREILPNLYMEGGVRR
                     LALDIHATVGSASAKRSPSYWDPLVGLTYRRQLGRKWRILIHGDGGGFGVGSDVDVTA
                     TARAEWQFARHFGIAMGYGGMHFSDSNTVDGQTLKISPTLHGPTFGLGIFF"
BASE COUNT          372 a          584 c          590 g          499 t
ORIGIN      
        1 gatgattgtg ggcgcagttg ccggtgtttt gggaccgaca gcagcccaat ctgtgccctc
       61 acgatgacga ctcgcctggc aactctcccc gtcaagcatc cgtcggccgc gcttctgttt
      121 gttcaaatca tgagcatttt gttgtatccg ctaatgggag acaagtcggc ggagcgtgcg
      181 ttgttcgggg ttatcggcct gctcgtgctc ggtgcggcac tatatgtcgt gaaacgcggt
      241 ccctggctga cggggttcgc cgtcatgctc gcactccccg tggttggcct ttctatctgg
      301 ctggcgttcc acttcgatcc cagcagaatg gtcatcatgg ccgcacttga ggccgtgttc
      361 tatttctacg caacgggcag cttgattgcc tacatgctcg aggacgaatt cgcaacggtc
      421 gacgaactgt tcgccgctgg ggcgacgttc acgctgctgg cctgggcatt cgcctatgtg
      481 tacgtggtgt gccaaggcct cctgccgggt agttttggcg ccctcactgg cccgggcaac
      541 cagcgcacgt ggatggagtt gctcttcctc agtttcacga cactgtccgg cgtcgggctc
      601 ggcgatatcg tacctctcct gccgatggcg cgcggcctcg tcatgattga ggaattctcg
      661 ggcgtgatgt acctcgccct ggtagtgtca cgattgattg cgctgtccgt aaagacgcga
      721 acggcgcggt gaatcagcgc tgaacgagcg atcttctggt tcagcgcgag ggagagctcc
      781 atggacgcat tcttcacttc atccggttta gtcgcgctca tcacgctgac gttccttgag
      841 atcgtgctcg gcgtcgataa tgtcatcttc atttcgatcc tggccagcaa acttccgagg
      901 tcgcagcaag acctggcgcg gcgaatgggg cttctggccg cgatgggaat gcgcatcctg
      961 cttttgcttt ccatcgtctg gatcaccagg ctgacggctc cgctttttca tgttttggag
     1021 cgccccatct ctggacgcga tctcatcctg atcgccggtg gcttgttcct gctcgcgaag
     1081 gcgacgatcg aaattcacga tcgcttggag ggcgaagaag gccacagctc ggcacgcgtg
     1141 cggccgtcat ttgccgctgt cattgctcag atcgtagtgc tggatatcgt cttttcgctg
     1201 gattcagtca ttaccgccgt cggtatggcc aacgaaatca ctgtcatggt gacagcggtg
     1261 atcctggccg tcggcgtgat gatgttctca gcggggccca tcagcacctt cgtcaatcgc
     1321 catcccacag tgaaagtcct cgcgctctcg tttttattgt tgattggcgt tgcgttggtt
     1381 ggcgacggac tcggcatgca catcccgaag ggctatatct actttgcgat gggcttctct
     1441 gtgtttgtgg aaatgatcaa cctgagggta cggagagcga ctgcgcctgt tcacctgcat
     1501 gagccgtaca tttagctcaa gattggcgta ggcgttatcg cacctttcct ggttgtcaac
     1561 atgaaggatt ccggctgcag ggctagaaga aaattcctaa tccaaaggtc ggcccgtgca
     1621 aggtaggact gatcttgaga gtctggccat ccacagtgtt gctgtcgcta aaatgcatgc
     1681 cgccgtaccc catcgcaatc ccgaaatgac gagcaaactg ccactcggca cgggccgttg
     1741 cggtcacgtc cacgtctgaa cctacgccga atcccccgcc atcgccgtgg attagaatcc
     1801 gccactttct gccaagctgt cgacggtaag tgagaccaac gagaggatcc caataactgg
     1861 gggaccgctt cgcgcttgct gacccgacgg tggcatgaat gtccaaggcc aacctccgga
     1921 cgccgccctc catataaagg ttggggagaa tttcgcggcc tgccatcgcg tttcccaaaa
     1981 taaagtcgag tttgacgtcg acgaatgggt ttttgcgttg agcggagaga gccgcccaca
     2041 taaac
//
LOCUS       DASTPM010000114         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_493930, whole genome shotgun sequence.
ACCESSION   DASTPM010000114 DASTPM010000000
VERSION     DASTPM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493930"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..588)
                     /locus_tag="VFR42_01970"
     CDS             complement(49..588)
                     /locus_tag="VFR42_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0367946.1"
                     /translation="MTKSLAAELELLANRMTSEGAGNQQLSIPALAERIAKNLNVKSE
                     EVAILVISEKQRSLQFLAPGALSNVGSIPLSSTSALAARTARESRPDILNNFTTVRHA
                     TVFEGVKADSLSANAIQKIISAPILCEGKVIGVIQISRKGASAVEAGPDFTPDDLGKV
                     LAICKPLGKLIRHAAGTGN"
     gene            844..1497
                     /locus_tag="VFR42_01975"
     CDS             844..1497
                     /locus_tag="VFR42_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367947.1"
                     /translation="MSVTTTRPDSSYLLRKLHSLSGVIPVGAFLAEHFWSNSAVLVSA
                     GKYDSVSQELQTIPFRPFVELAVIVLPILFHGGYGVYVWLRGKSNVSHYPWVGNWMYT
                     FQRYTGLVAFAFIAWHFYTERLLTGGRSTYQTVYADLQNPLFLAFFVVGILCSSFHLG
                     VGIWNFLCKWGLAATARAQRAAGQLGALVGVTFSLVGILILISFRFDWHPFSSYVTK"
     gene            1494..>2750
                     /gene="sdhA"
                     /locus_tag="VFR42_01980"
     CDS             1494..>2750
                     /gene="sdhA"
                     /locus_tag="VFR42_01980"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HEU0367948.1"
                     /translation="MKQPKIIVVGGGLAGLMATIKVAESGVPVDLFSIVPVKRSHSVC
                     AQGGINAAKNQKGEGDTTAKHFDDTIYGGDFLANQPLVKRMCEAAPGIIDLLDRMGVM
                     FNRTPEGLLDYRRFGGTLYHRTAFAGATTGQQLLYALDEQVRRHESEGKVRKYEGYTF
                     LSAVIDDAGICRGICAMDLRSMEVRTFAGDAVIFCTGGIGAIFGRSTNSVVCTGSAQS
                     ALFQQGVDYANGEFIQVHPTAIPGEDKLRLMSESARGEGGRVWVPKNPVDTRAAKSIP
                     QNERFYFLEEWYPKYGNLVPRDIATRAIHRIVYQEKLGLNGQAAVYLDVTHIPRETLD
                     RKLEGILEIYEKFLGVDPREEPMKVFPAMHYTMGGIWVNGEDQATSVPGIYAAGECEY
                     QYHGANRLGANSLVSCIFGGGLAGPAA"
BASE COUNT          558 a          826 c          726 g          640 t
ORIGIN      
        1 gtcaacgcaa aacgcgccgc tcaagcaaaa ttcatcgtgc tgcaaaaatt aatttcccgt
       61 tcctgctgcg tggcggatca atttgcctag cggtttgcag atcgccagca ccttgcccag
      121 atcatccggc gtaaagtccg ggcccgcttc gactgcgctc gctccttttc ggctgatttg
      181 aatcactcca atcaccttgc cttcgcacaa aattggcgcg ctgatgattt tttgaatcgc
      241 gttggcggaa agtgaatctg ccttcacacc ttcgaacacc gtagcgtggc gtaccgtcgt
      301 gaaattgttg agaatgtcgg gccggctttc ccgagcggtc cgcgcggcca atgccgacgt
      361 gctggaaagc ggaatggacc cgacgttgct cagtgccccg ggcgccagga attgcaggct
      421 ccgctgcttt tcggaaatca ccaggatcgc cacttcctcc gacttgacgt tcaaattctt
      481 cgcgatacgt tccgccagcg ccggaatcga caattgttga ttgccggcgc cttccgaggt
      541 catacgattc gcaagcagtt ccaattctgc ggcgaggctt ttcgtcatgg tggattaaag
      601 gccttgagag gaagtcacag tttttcggcg ggcgcgcgcc gtgttgcaac atctccaaca
      661 taacagcaat accgtcgcaa aagcgcgaca ttttcttgcc gagtttcgag aaatttgaaa
      721 cctcctcttt ggctttgatt ttgcttcgct gcgcgcaact gatataattt gtttgctgca
      781 ctttgagtcc ttccgcctga gactctagcg agctcggagc agaaaagttg cagcggagag
      841 ggcatgtccg taacgacaac ccggcctgac agtagttacc tcctcagaaa actccattct
      901 ttgagcggcg tgattcccgt cggtgcattt ttggcggaac acttctggtc caacagcgcc
      961 gttctggtca gcgcaggcaa gtacgattcc gtttcgcaag agctgcagac cattcctttt
     1021 cgtccttttg tcgaactcgc ggtcatcgtt ctacccatcc tgtttcacgg cggctacggc
     1081 gtttacgtct ggctgcgcgg caagtccaat gtgtcgcact acccatgggt cggcaactgg
     1141 atgtacacct tccagcgcta caccggcctc gtcgctttcg catttatcgc ctggcacttt
     1201 tataccgaac gcctgctgac gggaggccgc tccacttacc aaacggttta cgcggatttg
     1261 cagaatccgc tctttctggc atttttcgtc gtcggcatcc tctgttcgtc attccatctc
     1321 ggcgtcggta tctggaattt tctctgcaaa tggggactcg ctgccaccgc acgcgcgcag
     1381 cgcgctgccg gtcagcttgg cgcgctcgtc ggtgtgactt ttagtctcgt gggaattttg
     1441 attttgatca gttttcgttt cgattggcat ccgttttctt cgtacgtaac gaaatgaaac
     1501 agcccaagat cattgtcgtc ggtggaggac tggctggact gatggcaacc atcaaggtgg
     1561 ccgagtccgg cgttccagtc gatcttttct cgattgtccc ggtaaaacgc tcgcactcgg
     1621 tctgcgcgca aggcggcatc aacgcagcaa agaaccaaaa aggggaaggc gacaccaccg
     1681 ctaagcattt tgatgacact atctacggcg gcgatttcct ggcgaatcag ccactggtca
     1741 agcgaatgtg tgaagccgcg cccggcatca tcgatcttct cgatcgcatg ggcgtcatgt
     1801 ttaaccgcac gccagaaggc ttgctcgact accggcgttt cggcggcacg ctttaccatc
     1861 gcaccgcctt cgccggcgcg accaccggac agcaactgct ctacgccctc gatgaacaag
     1921 tccgccgcca cgaatccgaa ggcaaagtaa gaaagtacga gggctatact tttctctcgg
     1981 ctgtaatcga cgacgcaggc atttgccgcg gtatctgcgc catggatttg cgctctatgg
     2041 aagtccgcac cttcgccggc gacgcagtta ttttctgcac tggcggtatt ggcgcgatct
     2101 tcggccgctc caccaactcc gtcgtttgca ccggctccgc gcaatccgca ctgtttcagc
     2161 agggcgtgga ctacgccaac ggcgaattta tccaggttca tcccaccgcg attcccggcg
     2221 aagacaagct ccgtttgatg agcgaatccg cgcgcggcga aggcggccgc gtctgggtgc
     2281 caaaaaatcc ggtagacacc cgggcggcga aaagcattcc gcagaacgaa cgtttctact
     2341 tccttgaaga gtggtatccc aagtacggca acctggttcc gcgcgatatc gccacccgag
     2401 ccattcatcg cattgtctac caggaaaagc tcggcctcaa cggacaagcg gccgtttatc
     2461 tcgacgtcac gcacattcca cgcgaaacgc tcgaccgtaa gctggaaggc attctcgaaa
     2521 tctacgaaaa attcctcggc gtcgacccgc gcgaagagcc gatgaaagtt tttcccgcca
     2581 tgcactacac catgggtgga atctgggtga acggcgagga ccaggccacc agcgtccccg
     2641 gcatctacgc cgccggcgaa tgcgaatacc aatatcacgg cgcgaatcgc ctgggagcca
     2701 actcgctcgt ctcctgtatc ttcggcggcg gactggccgg gcccgctgcc
//
LOCUS       DASTPM010000115         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_955284, whole genome shotgun sequence.
ACCESSION   DASTPM010000115 DASTPM010000000
VERSION     DASTPM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955284"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1649)
                     /gene="lpdA"
                     /locus_tag="VFR42_01985"
     CDS             complement(225..1649)
                     /gene="lpdA"
                     /locus_tag="VFR42_01985"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0367949.1"
                     /translation="MADNSSLHLAVVGGGPGGYAAAFLAADLGLKVSLIDPEVNPGGV
                     CLYRGCIPSKALLHVAKLIEESHQAKNWGIEFSDPKIDLAKLRGWKESVVQRLTAGLG
                     QLSKQRKVQYIQGRAGFENSTTLRVTKADGSAESLRFDRVLLATGSRPAVIPTLKIDS
                     PRMLDSTGALDLNDIPKTLLVVGGGYIGLELGSVYAALGTRVTVVEMLSGLLPGADRD
                     LVLPLHKRLEKMFDGILLNTTVKSFKEESGGIRATFDGADVKEREKVFDKVLVSVGRK
                     ANSEIPGLDKTQVKVGPKGFIQVNKQLQTDDPSIYAIGDVVGEPMLAHKASHQGRTAV
                     EAIAGHKVAFEPHAIPAVVFTDPEIAWAGLTETQAKESGRDIAVAKFPWGASGRAITL
                     DRPEGMTKLILDPKTERVLGVGIVGVGAGELISEGVLAIEMAALAGDLELTIHPHPTL
                     SETVMEAAEVFFGTSTDVYRPKRG"
     gene            complement(1671..3413)
                     /gene="aceF"
                     /locus_tag="VFR42_01990"
     CDS             complement(1671..3413)
                     /gene="aceF"
                     /locus_tag="VFR42_01990"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEU0367950.1"
                     /translation="MTEFKLPELGENIEQGDLVRLMIAPGADVREGQPVMELETDKAV
                     IEVPSSVSGVVRDIRVKEGDKLKVGQVIFTVENGAGAKAQAQQAQAPPPKTPAPSAPA
                     QIERPAPASPGPAQPPSSPAAASRTPSGASQFTLPELGENIDQGDLVRLMIAPGAKVS
                     EGQPVMELETDKAVVEVPSSVSGVVKEIKVKEGDKVKVGQVIFTLEGGAQPQPAKAAS
                     EPVEHVSGQQAARLAFHLAMKAEGKTEEQALPPDRPQPAPPAFSMPVQLGKVAGTEHR
                     DPVPAAPHVRRLARELGLDIYNVTGTGPGGRISEDDVKACAKATLTAAVSAAQAPQAA
                     RFAEPKLPDFTKWGKVERVSMRGVRRKTAEHLREAWVTIPHVTQHDRADITELEQLRA
                     KFAPRAEEAGGKMTVTAIALKVCASALKVFPQFNASIDMEKEEIVYKQYINIGVAADT
                     DRGLLVPVIRDVDKKNIVELAAELSQFSKKARDKKLTPEEMEGGTFTITNLGGIGGTA
                     FTPIVNHPEVAILGLSRGRMEPEWINGKFEPRLILPLSLSYDHRLIDGADAARFLRWI
                     AEAFEQPFLLSVQG"
     gene            complement(3430..>3716)
                     /gene="aceE"
                     /locus_tag="VFR42_01995"
     CDS             complement(3430..>3716)
                     /gene="aceE"
                     /locus_tag="VFR42_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEU0367951.1"
                     /translation="DVPGPFVAASDYMKVLPESIARWVPGRLVSLGTDGFGRSENRAA
                     LRDFFEVDAKHIVLATLSALARENQIKMETVQQAVKDLGINPEKPNPAIS"
BASE COUNT          678 a         1101 c         1125 g          812 t
ORIGIN      
        1 ttcggcgtct gcctcccgag aggatcactg ggcgcagctt tttctttctg ggcttccgcg
       61 ccttgcagaa tctgggtgat ttgcgccgag gaagagaggc tgagagcgag gcagagaatc
      121 agaagcgaga ttttgcggaa agcattcatc caacttcctt cgaacggtgc gcgggcagca
      181 gacgccttcg cgcggtgctg tctagttctg ttggtcagtg cctgtcagcc gcgctttggg
      241 cgatacacgt ccgtgctggt gccgaagaaa acctcggcgg cttccatcac ggtctcagag
      301 agcgttgggt ggggatggat cgtcaattca aggtcacccg ctagtgcagc catctcgatg
      361 gcgaggaccc cttccgaaat caactctcct gctcccaccc caacgatacc cactcccagc
      421 acgcgttcgg tttttggatc tagaatcagc ttggtcattc cctccgggcg gtcgagcgtg
      481 atggcgcgtc cggatgcacc ccagggaaac ttggccacgg caatgtcgcg gccggattct
      541 ttcgcttggg tctccgtgag tcccgcccag gcaatctcgg gatctgtaaa taccaccgcg
      601 ggtatggcgt ggggctcgaa ggcaactttg tgtccagcga tcgcttcgac ggcagttctg
      661 ccctggtgcg aggccttgtg cgcgagcata ggttcgccca ccacgtcgcc gatcgcatag
      721 attgaaggat catcggtctg caactgcttg ttgacctgaa tgaagccctt gggcccaacc
      781 ttgacttgag tcttgtcgag tccgggaatt tccgagttcg cctttcggcc gaccgacacc
      841 aggactttgt caaagacctt ctcgcgctcc ttgacgtcgg ccccatcgaa agtggcgcga
      901 atgcctccgc tttcttcttt gaaggacttg acggtggtgt tcagcaggat gccatcaaac
      961 attttttcta gccgcttgtg gagcggcagc accaggtcgc gatcagcgcc gggcaacaat
     1021 cccgacaaca tttcgacgac ggtcacgcgc gttcccagtg cggcatagac cgatccgagt
     1081 tccagtccaa tgtagccacc acccaccacg agcagagtct taggaatgtc gttaaggtcg
     1141 agagccccgg tggaatcgag catgcgcggg ctgtcgatct tcaacgtggg aatcaccgcg
     1201 gggcgggagc cagtcgccag tagcacgcga tcgaagcgaa gcgattcggc gctgccatcg
     1261 gctttggtga cgcgcagcgt ggtcgagttc tcgaatccgg cgcgcccttg aatgtactgc
     1321 accttgcgtt gcttagatag ctggcccaac cctgcggtca ggcgctggac cacactctct
     1381 ttccagccgc gaagcttggc gagatcaatc ttcggatcgc tgaactcgat gccccagttt
     1441 ttcgcttgat gggattcttc gatgagcttt gcaacatgca gaagggcttt ggagggaatg
     1501 cagcctctgt acaagcagac gcccccgggg ttcacttccg gatcgatcaa actaactttc
     1561 aggccaagat cggccgctag aaaagcggcg gcgtatccgc ccggtccgcc gcccacaact
     1621 gcgagatgca agctggaatt gtctgccatg aatttgctgg tccttaagaa ttaaccctga
     1681 accgacagca ggaaaggctg ctcgaacgct tccgcgatcc aacgcaggaa gcgagccgcg
     1741 tcggcgccgt caattaaccg atgatcgtac gagagtgaca gcggcaggat tagccgaggt
     1801 tcgaatttcc cgttaatcca ctccggctcc atacgcccgc gtgacagtcc gagaatcgcg
     1861 acttccgggt gattgacgat tggagtgaac gctgtgccgc cgatgccacc cagattcgta
     1921 atcgtgaagg taccgccttc catttcctcg ggggtgagct tcttgtcgcg agcttttttg
     1981 gaaaattgcg agagttcggc ggcgagctcg acgatgttct tcttgtccac gtcccggatt
     2041 accgggacga gcagtccgcg gtcggtatcg gccgcgactc cgatgttgat gtattgcttg
     2101 tagacgattt cttccttctc catgtcgatg gaggcattga actggggaaa gactttcagc
     2161 gcagaggcgc agactttcaa agcgatggcg gtgaccgtca tcttgccgcc ggcttcttcg
     2221 gcgcggggcg cgaacttggc gcgcagctgt tcgagttcgg tgatgtcggc gcgatcgtgc
     2281 tgcgtcacat ggggaatcgt gacccaggct tcgcgcaaat gttcggcggt cttgcggcga
     2341 acgccgcgca tggacacgcg ctcgaccttt ccccacttgg tgaagtcggg aagctttggc
     2401 tcggcgaagc gcgcggcctg cggtgcctgg gcggcggaga cggcagccgt aagcgtcgct
     2461 ttcgcgcacg ctttcacatc gtcttcgctg atacgtccac cgggaccggt accggtcacg
     2521 ttgtagatgt cgagaccaag ttcgcgcgcc aggcggcgca catgcggagc agccggaaca
     2581 gggtcgcgat gctcggtccc agcaactttg ccgagttgca cgggcatgga gaaggcggga
     2641 ggcgcaggct gcggtcggtc cggaggcaga gcctgctctt cggttttgcc ctcggccttc
     2701 atcgcaagat gaaacgccag ccgtgcggct tgttgtccgg aaacgtgctc gacaggctcg
     2761 gatgcagctt ttgcgggctg cggctgcgca ccaccctcga gcgtgaagat gacctggccg
     2821 actttgacct tgtcgccttc tttgactttg atctccttga caaccccact gacggatgaa
     2881 ggcacctcga cgacggcctt gtcggtctcc agttccatga cgggctggcc ttcggagacc
     2941 ttcgcacccg gagcgatcat cagccgaacg aggtcaccct gatcgatgtt ctcgcctagt
     3001 tcgggaagcg tgaattgaga agctcctgat ggcgtgcgcg aagctgcggc gggggacgag
     3061 ggcggctgag cggggccagg cgatgccggc gccgggcgtt cgatctgtgc cggtgctgag
     3121 ggtgctggcg ttttcggcgg aggagcctgg gcttgctgag cttgcgcctt tgcgccggcg
     3181 ccattctcga ccgtaaagat cacttgcccg accttcagct tgtcgccttc cttcacgcgg
     3241 atatcgcgta ccacaccgct caccgacgac ggtacttcga tcacggcctt gtccgtttcc
     3301 aactccatca ccggctggcc ttctcgcaca tcggcaccag gcgcgatcat caggcgaacc
     3361 aggtcgcctt gctcgatgtt ttcgcccagc tcgggcagtt tgaattccgt cactgtactc
     3421 aaacctttct tagctgatgg ccggattcgg cttctcggga ttgatgccga gatccttcac
     3481 ggcttgctgt acggtttcca tcttgatttg attttcacgg gctagtgcgc tcagcgtggc
     3541 gagcacgatg tgcttggcat cgacttcaaa gaaatcgcgc aaagcggcgc gattctcgct
     3601 gcggccaaac ccgtcggtgc caagtgaaac caaacgtccc ggaacccagc gggcgatgct
     3661 ctccggcaac actttcatgt agtcggaagc ggcaacgaac gggcccggca cgtcct
//
LOCUS       DASTPM010000116         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_955305, whole genome shotgun sequence.
ACCESSION   DASTPM010000116 DASTPM010000000
VERSION     DASTPM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955305"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..702
                     /locus_tag="VFR42_02000"
     CDS             91..702
                     /locus_tag="VFR42_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0367952.1"
                     /translation="MEPRETTTRVSSAAQLSPDDSALAAAWQAGDLRAFERLYRLHGG
                     RMKSMARNLLGTASDAEDAVQETFLKIQRSISSFRGQSSFRTWSFRILINTCYDVRRS
                     RMRKKEVSQDEPEGAESAPRPEPRAPGSHPSLRLALERAVAQLTQQQREVFLMYEVEG
                     LRHSEIAAVLEISETASKNTLFQAKKNLRQMLEAPRSSAAEAR"
     gene            708..1370
                     /locus_tag="VFR42_02005"
     CDS             708..1370
                     /locus_tag="VFR42_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367953.1"
                     /translation="MNISCNDRERIFLDGTEEEWAALEVHAATCVACREEMNAWKSLS
                     VVASELHEEWDSPTLWPRIERALAKQSTSSQWSWWGRFFSGWNVSSLQWQTAAASLVL
                     VAVTSSAVWFVMKSRGTVDSPTPTLLKDEAVRSVERAEAVYEQAIDKLDLQARAQLEN
                     ASTPLMASYREKLQVLDSAIADLKLQAGVNPANGHLRRQLLAMYQEKQSTLEEVLEAK
                     PQ"
     gene            1418..2896
                     /locus_tag="VFR42_02010"
     CDS             1418..2896
                     /locus_tag="VFR42_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367954.1"
                     /translation="MAVLGAIFVLLAASAFGRGAAQNEVTRDFQKTVTLSGNQGVSVD
                     HRFGKVMVHGESGREVKISAIIRVQDSNANDAQEFLQKIQIDVREEGDGVHIRTIYPE
                     NKLPHIRIGGHSSFSVDYDIAMPTEAPLWLRNAFGNAEINGVRGWAQLENSNGGLLVR
                     DAGGAKLVNAFGKIELTNAAGNCSITNNNGAVNVTNVKGTLELRNRFGEIVASQISGA
                     ATISGGNGRVSLTQAGGNSLVSNSFGEVVVRNIAGNLSVTNSNGRIDVAEVSGNAELT
                     TSFGAVEATRVGGATIKDSNGSVHVTEAQGFVSVNSSFGTVKGSQLSKGANVVTGNGS
                     IELSNVQGEVYAKTSFGSIYAENVKGKFTAQDSNGSVTAKAIEGDASVTTSFSGVSLE
                     GVGGKISVENQNGAVDVTASSATCKDVSIRTSFSHIGVRLPTNAGYRVKARTSFGRIN
                     SQLPITASGAMGGDSLEGTIGNGGCALDLTNSNGNIDISRAQ"
     gene            complement(2915..3391)
                     /locus_tag="VFR42_02015"
     CDS             complement(2915..3391)
                     /locus_tag="VFR42_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0367955.1"
                     /translation="MSSWREYPEKPIVGVGGVVIEEGRALLIRRGSEPLLGQWSIPGG
                     TLELGETLVEGTERELKEETGLVVGILDLIEVFERINVGRGADETWTTLEEKRRPRFH
                     FVIVDYLCQRISGDAVAGGDVTDVAWASETELDKFGLTPTATRVIRKAFSLARNQK"
     gene            3587..>3805
                     /locus_tag="VFR42_02020"
     CDS             3587..>3805
                     /locus_tag="VFR42_02020"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367956.1"
                     /translation="MIRCPDCGAEIDVDEDEVEEGEILSCPECESELEVTQTHPVHVN
                     PISDDLDDEEDEEEEDDEAEEEEEDEEDL"
BASE COUNT          860 a         1041 c         1185 g          719 t
ORIGIN      
        1 tctaaacctt aggagccgcg aatgggaacg atgggacttg cgctgccctg ggcagctaga
       61 atcactcagg agcaggcacc aagggggcga gtggaaccaa gggaaacgac cacgcgagtt
      121 tcctccgccg cgcaattgtc gccggacgat tccgctttgg cggcggcgtg gcaagcggga
      181 gacctgcgtg cgttcgaacg tctgtatcgt ctgcacggcg ggcgcatgaa atcgatggcg
      241 cgtaacctgt tgggtaccgc gagcgacgct gaagacgcag tgcaagagac cttccttaaa
      301 atccagcgca gcatcagcag ctttcgcggc cagtcgagtt ttaggacgtg gtcgtttcgc
      361 atcctgatca acacgtgtta cgacgtgcgg cgcagccgga tgcgcaagaa agaagtttcg
      421 caggacgaac cggaaggcgc agagagtgcg ccgcggccgg agccgcgcgc gccgggttcg
      481 catccctcgc tgaggctggc gctggaacgc gctgtcgcgc agctgacgca gcagcagcgt
      541 gaagtttttc tgatgtatga ggtggaaggt ttgcgtcact ccgaaattgc ggcggtactg
      601 gaaatcagcg agaccgcttc gaaaaacacg ctcttccaag ccaagaaaaa tttgcgccag
      661 atgctggaag cgccgcgcag cagtgcggca gaggccaggt aatcgccatg aatattagtt
      721 gcaatgatcg cgaacggatt ttcctagatg ggacagagga ggagtgggct gcgctggagg
      781 ttcatgccgc cacttgtgtg gcgtgccgcg aagagatgaa tgcctggaag agcctgtcgg
      841 tggtcgcaag cgagttgcat gaggaatggg attcgccaac actgtggccg cggattgagc
      901 gggcgctggc gaagcagtcc acatcgtcgc aatggtcgtg gtgggggcgt ttcttcagcg
      961 gctggaacgt tagctcgctg caatggcaga ccgcagcggc ctcgttggta ctcgttgctg
     1021 ttacaagctc cgcagtgtgg tttgttatga agtcgcgagg gacagtcgat tctccaacac
     1081 cgacccttct gaaagatgag gcggtgcgga gtgtcgaacg cgcggaagcg gtttacgagc
     1141 aagcgattga caaactggac ctgcaggcgc gcgcacaact tgaaaatgcg tcgaccccgc
     1201 taatggccag ctaccgcgaa aagctgcaag tgctggacag tgcgatcgcc gatttgaaat
     1261 tgcaggcggg cgtgaatccc gcgaacggcc acctgcgccg gcagttgctg gcgatgtacc
     1321 aggaaaagca gagcacactt gaagaagtct tggaggcaaa accacagtga ttcacccagc
     1381 ctattattcc ccgtgcatgg ccccattgcg gcgagccgtg gcagtccttg gagcaatttt
     1441 cgtactgctt gctgcttcgg cgttcggccg tggcgcagcg caaaatgaag tgactcgcga
     1501 ctttcaaaag acggtcacgc ttagcggtaa tcagggcgtc agcgtggatc accggttcgg
     1561 aaaagtgatg gtgcacggcg aaagcggccg cgaggtgaaa atttccgcca tcatccgcgt
     1621 gcaggattcg aacgccaacg acgcgcagga gtttctgcaa aagattcaga tcgacgtccg
     1681 cgaagaaggc gacggggtgc acatccgcac catctatccg gaaaacaagt tgccgcacat
     1741 tcgcatcgga ggccactctt cattttccgt ggattacgac attgctatgc cgacggaagc
     1801 tcctctatgg ctgcgcaacg ccttcggcaa tgcggaaatc aacggagtgc gcggctgggc
     1861 gcagctggaa aactccaatg gagggcttct ggtgcgcgac gccggcgggg caaagctggt
     1921 caacgccttc ggcaaaatcg aactcaccaa cgcggccggc aactgcagca tcaccaacaa
     1981 caatggcgca gtaaacgtga ctaacgtgaa gggcacgctg gagctgcgca accgcttcgg
     2041 tgaaatcgtg gcttcgcaaa tcagtggcgc ggcgacgatt tccggcggca atggacgcgt
     2101 ttcactgacc caggcaggcg gcaattcgtt ggtgagcaac tcgtttggcg aagtggtggt
     2161 gcggaacatt gcgggaaacc tcagcgtaac caacagcaac ggccggattg acgtcgccga
     2221 agtttcgggc aacgcggagc tgaccaccag cttcggcgct gtggaagcga cgcgcgtggg
     2281 cggcgcaacg atcaaagaca gcaacggttc cgtgcatgta acggaagcgc agggatttgt
     2341 ttcggtgaac tccagtttcg gcacagtgaa aggcagccag ctatccaagg gcgcgaatgt
     2401 ggtgaccggc aacggctcga tcgagctgag caacgtgcag ggcgaggttt acgcgaagac
     2461 gtcgtttggc tcgatttacg cggaaaacgt gaaaggcaag ttcacagcgc aagattcgaa
     2521 cggttcggtg accgcaaaag cgatcgaggg cgacgccagc gtgacgactt ccttttccgg
     2581 tgtttcgctg gaaggcgtgg gcgggaaaat cagcgtggaa aaccagaacg gcgccgttga
     2641 cgtgaccgcg tcgagcgcca cttgcaagga cgtcagtatc cgcacatcgt tttcgcacat
     2701 cggagtgcgg ctgccgacga atgccggtta tcgcgtcaag gccagaacgt cattcggcag
     2761 aatcaactcg caactgccga tcacggcgtc gggcgcaatg ggcggcgatt cgctggaagg
     2821 caccattggc aacgggggct gcgcgctcga tctgaccaat tcgaacggca acatcgacat
     2881 ttccagagcg cagtagatac gcggactcgg tttgctactt ctggtttctc gccagagaaa
     2941 aagctttgcg gatcacacgg gttgcggttg gcgtcagtcc aaacttgtcc aactcagttt
     3001 cgctagccca agccacgtcg gtaacatcgc ctccggcaac tgcgtcgccg gatatccgct
     3061 ggcacaagta atccacgatg acaaaatgaa atcgcggccg ccgcttctct tcgagagtgg
     3121 tccaggtttc gtcggcgcca cgcccgacat tgatgcgttc aaaaacttcg atcaggtcaa
     3181 gaatccccac aacgaggccg gtttcctctt tcagctcgcg ttcagtgccc tccacaagcg
     3241 tttcgccgag ttccaaggtg ccgcctggaa tggaccactg tcccaggaga ggttcactgc
     3301 cccggcggat cagcagagcg cgcccttctt caatcaccac accgcccacg cccacaatcg
     3361 gtttttcagg atattcccgc caggaactca tgcagccccg ctctctacag gcttctcccc
     3421 ggtaatattc ggggcggccg tccccacgaa acagttgatt tctcgcgctg cggtatataa
     3481 tacgacggtc ggcggcggtc gcgattctcc ctggtcccgc cccgcgaatt agggtttcag
     3541 ggtttgtttg cgaaccctga actaaaattg tccggaggat cgtgccgtga ttcgctgccc
     3601 cgattgtggc gccgaaattg acgtggatga ggacgaagta gaggagggcg agatcttgag
     3661 ctgccccgaa tgtgaatccg aattggaagt cacgcagacg catcccgtcc atgtgaatcc
     3721 tatttccgac gacctcgatg atgaagaaga tgaagaggaa gaagacgacg aagccgagga
     3781 agaggaggaa gacgaggaag acctc
//
LOCUS       DASTPM010000117         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_926480, whole genome shotgun sequence.
ACCESSION   DASTPM010000117 DASTPM010000000
VERSION     DASTPM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926480"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VFR42_02025"
     CDS             <1..703
                     /locus_tag="VFR42_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271413141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEU0367957.1"
                     /translation="ATKPPDYQPKPGDEGDDALAGDKPFILHADGLGWIWVPAGLNDG
                     PLPAHYEPLESPIPNPIYPSRQANPVANRRTRPGNEYASSPDSRFPHVLTTYRLTEHH
                     TAGGMSRTLSHLAELQPELFCELSPELGNQLSISNGDWVTVITRRGAVEARALITRRM
                     KTLTIDGRTVQQVGLPFHFGYTGLVKGDISNDLIAISEEPNVRIMETKGLLCTVLPGT
                     RATNAHHLESLRPPS"
     gene            712..1491
                     /locus_tag="VFR42_02030"
     CDS             712..1491
                     /locus_tag="VFR42_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0367958.1"
                     /translation="MTKNGFLTDASICIGCKACEVACKEWNDVPADGMELSGLSYDNT
                     KALGASTWRHVLFLEQPLTAGPQLADTQNPFRWVFLSDVCKHCANAGCLEACPTGSIV
                     RTDVASVYVQPDICNGCGYCVVSCPFGVIDKRPGDGRAFKCTFCYDRQKVGLMPACAT
                     ACPTQSIQFGPFDELQERAMKRVAELKDRGYSDARLYDARDTSVGGIHSLSIILGQPE
                     NYNLPPAPEVPTAYLKSAWTSAFLSAAILVAVVCGAFALRL"
     gene            1419..>2248
                     /gene="nrfD"
                     /locus_tag="VFR42_02035"
     CDS             1419..>2248
                     /gene="nrfD"
                     /locus_tag="VFR42_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU0367959.1"
                     /translation="MDLGIPIRRDPGCGRLWRFRAALMTGRRTTEERLHEIRRTAAQN
                     QGRGGAASHPQIRGHDSYYGMPALKPPVWTWEVPLYFFIGGIAGVSSCIAFVAHIFQA
                     SPALIRLSLWMGLIGAAICPMLLIADLGRPSRFLNMLRVFKLRSPMSMGAWILVAFSG
                     CAFLAVAAKELSLLGYASPLVSGIDWLGEASGALMGLLLASYTGVLIGATAIPVWFTH
                     RRFLPAHFLTSGLGGASAILELSGFFVPATQVLGFFTAGLETIFEILFAIRKRDVDAP
                     L"
BASE COUNT          429 a          659 c          662 g          498 t
ORIGIN      
        1 cgcgacgaaa cctcccgatt accagcccaa acccggcgac gaaggcgacg atgcactggc
       61 aggagacaag ccctttatcc tgcatgccga cgggctcggt tggatctggg ttccggcagg
      121 gctgaacgat ggcccgcttc ctgcgcacta tgaaccgctg gaatcgccga ttccgaatcc
      181 gatttatcct tcgcggcagg caaatccggt ggctaaccgg cgcacgcgcc ctggaaacga
      241 atatgccagt tcacccgaca gccgattccc gcacgtgttg acaacctatc ggttgaccga
      301 acatcatact gccggcggca tgtcacgcac gctgtcgcat ttggcggaac ttcagcccga
      361 actgttttgc gaattgtcgc cagagttggg taaccagctt tccatctcga acggagactg
      421 ggtgaccgtt atcactcggc ggggcgcggt cgaggcgcgc gcactgatta cacgccggat
      481 gaagacttta acgatcgacg gacgaacggt ccaacaagta ggcctgccat ttcacttcgg
      541 atataccgga ttggtgaagg gcgacatcag caatgacttg attgctatct ccgaagaacc
      601 caacgtgcgc ataatggaaa cgaaagggct cctttgcacg gtgttgccag gtacgcgcgc
      661 caccaatgca catcatctcg aaagtcttag gccgccttcc taaaaacgcc cgtgaccaaa
      721 aacggattcc tcaccgacgc aagcatttgc ataggctgca aggcatgcga ggtagcttgc
      781 aaggaatgga acgacgttcc cgccgacgga atggagttga gcggcctctc gtacgacaat
      841 acgaaggctt tgggcgcgtc cacctggcgt catgtgcttt ttctggagca accgctcact
      901 gccggtccgc agcttgcgga tacgcagaat ccttttcgct gggtctttct atccgatgtt
      961 tgcaagcatt gcgccaacgc cggctgtctt gaggcctgtc caaccggctc tatcgttcgc
     1021 accgatgtcg cctcggttta cgttcaaccc gacatttgca atggttgcgg atattgcgtg
     1081 gtttcgtgcc cgttcggcgt gatcgataag cggccgggcg acggccgagc cttcaaatgc
     1141 acattttgct atgaccgtca gaaagtgggt ttgatgcctg catgcgcgac ggcgtgtccc
     1201 acacaatcta ttcagtttgg tccgtttgac gaactccagg agcgcgcgat gaagcgcgtg
     1261 gccgaactta aggaccgggg atattcggat gcgcggctgt acgacgcgcg agacaccagc
     1321 gtgggcggca tacactcgct ctctatcatt ctcggccagc cggaaaacta caatctgccg
     1381 ccggcgcccg aagtcccgac cgcatacttg aaaagcgcat ggacctcggc attcctatcc
     1441 gccgcgatcc tggttgcggt cgtctgtggc gctttcgcgc tgcgcttatg accgggcgga
     1501 gaacgaccga agagcggctg cacgaaattc gcaggacggc ggcgcaaaac cagggccggg
     1561 gcggcgcggc gagtcacccg cagattcggg gacacgacag ttactacggc atgccggcat
     1621 tgaagccacc cgtgtggacg tgggaggtgc cgctctattt cttcatcggg ggaattgcgg
     1681 gagtctcttc ttgcatcgca ttcgtggcgc atatttttca ggcctcaccg gccttgattc
     1741 gcctttcact atggatgggc ctgatagggg cggccatttg cccgatgctg ctgatcgccg
     1801 atcttggccg cccgagccgg ttcctcaaca tgcttcgcgt attcaagctc cggtccccca
     1861 tgtcgatggg cgcctggatt ctcgtcgcct tctcaggctg cgcgttcctg gcggttgccg
     1921 ccaaggaact ttcgctgctc ggctatgcct ctccactggt gagcggaatt gactggctgg
     1981 gggaagcgtc gggagcgctg atgggtcttc tgctggcaag ctacaccggg gtgttaatcg
     2041 gcgcgacggc aattcccgtc tggtttacgc atcgcagatt tcttcccgct catttcctga
     2101 cttcgggact tggcggagca tcggccatct tagagctgag cgggttcttc gttccggcta
     2161 cgcaggttct cggttttttc acggcggggt tggagacgat cttcgaaatc ctttttgcga
     2221 ttcgtaagcg ggacgtggat gcgccgct
//
LOCUS       DASTPM010000118         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_407667, whole genome shotgun sequence.
ACCESSION   DASTPM010000118 DASTPM010000000
VERSION     DASTPM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407667"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..327)
                     /locus_tag="VFR42_02040"
     CDS             complement(169..327)
                     /locus_tag="VFR42_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367960.1"
                     /translation="MKTIVEVLRAKEEEIARVRKEVEALRIAAGLLSDQDNATDRKSE
                     YRPLLQMP"
     gene            complement(437..880)
                     /locus_tag="VFR42_02045"
     CDS             complement(437..880)
                     /locus_tag="VFR42_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU0367961.1"
                     /translation="MKLRIAWIGKTKNQAIQSLTDEYLKRIQRYADAEGVSLADEAAL
                     LKLREKSATRPAHTIVLMEAGGKQLSSEDFAQFLQNHQDRNPQPLVLAIGPADGFSAA
                     ARKSAAVCLSFGKMTLAHELARVVLLEQVYRALTILHRHPYHTGH"
     gene            complement(925..1350)
                     /gene="rsfS"
                     /locus_tag="VFR42_02050"
     CDS             complement(925..1350)
                     /gene="rsfS"
                     /locus_tag="VFR42_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU0367962.1"
                     /translation="MKTNETRRQVSEAITACQEKKAEEITILELEKDSGAFTDYFVVC
                     SGTNPRQIQAIADEVELRLKKAGLYPAHIEGYRQAEWVLLDYVDFVVHVFAEKARRYY
                     ELERLWKSAKRLEPADLKTPARRAVKKRSDASTARRKRA"
     gene            complement(1438..>2000)
                     /locus_tag="VFR42_02055"
     CDS             complement(1438..>2000)
                     /locus_tag="VFR42_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HEU0367963.1"
                     /translation="MVALATAPDKALVPSLLEAPIEDVPGPSKAVAISSKSAPAKHPD
                     ANYTVDTLRRLKQSLKKIDEIFFLIGIDAFRDIAKWHDAEELFGLCEFIVASRPGYSL
                     ADVANSLPEKLRPAAHITKPFEKQPARGDLVLNGATVHLLDGVHHAISATAIREAVTA
                     GKPLGRLVDPAVADYIKKCELYKRAT"
BASE COUNT          392 a          569 c          583 g          456 t
ORIGIN      
        1 cggtcgtcac cgcgcgcgcc gatgtgcgtg gcaaagagtt aggcgaagag ttcagcggac
       61 cataccgctt cacacgggtc tgggtacggc ggaacggcca ctggcagaca gtgagctatc
      121 aggcaaccgt aacccgataa agatttcaac gcggttcggg agcagtcact atggcatttg
      181 cagcaggggc cgatactccg atttgcgatc ggtggcgttg tcctgatcac taagcagtcc
      241 ggcggcgatt cttaaagctt ccacttcctt gcgtacgcgc gcgatttcct cttccttggc
      301 gcgcagaact tccacgatcg ttttcatgat gaatccttgc ggcggggctg gaatttgtgg
      361 gcaacttctc cgccacgagc ctgaaatcta cgccgcggga agcactagtg gtacgtctca
      421 gaagtacatg ttggccttag tggccggtgt gataggggtg gcgatgcaag atcgtaagag
      481 cacggtacac ctgctccagc aatacgacac gggccagctc gtgggcgagg gtcatcttcc
      541 caaatgatag acatacggcc gccgacttgc gcgccgccgc ggagaatccg tccgccgggc
      601 cgatggccaa caccaggggt tgcgggttgc gatcttgatg gttttgcaga aactgggcaa
      661 aatcttcgga cgaaagctgc ttcccaccag cttccattag cacaatcgtg tgcgcgggcc
      721 gggtagcgga cttttcccgt aacttgagga gcgcggcttc gtcagccagc gaaacgcctt
      781 ccgcgtcggc ataccgttga atgcgcttga gatattcatc ggtaagcgac tggatggctt
      841 ggttcttggt cttgccgatc caggcgattc gcaatttcac ggaagcgatt taaccacaag
      901 caaggcggaa aactggccgc aggactacgc cctctttcgc ctggcggtcg atgcatcgct
      961 gcgttttttc acagctcgtc tggctggagt cttcagatca gcgggctcga gacgcttggc
     1021 cgatttccac agacgctcga gttcgtagta tcgccgcgcc ttctcggcga aaacgtggac
     1081 cacgaagtcc acatagtcga gcagaaccca ctccgcctga cgatagccct ctatgtgggc
     1141 tggataaagt ccggcctttt tcagacgcaa ttccacctcg tcggcaattg cctggatctg
     1201 ccgcggattc gtgccgctgc acacgacaaa gtagtccgta aacgcaccgg agtccttttc
     1261 cagttctaga atcgtgattt cttcggcctt cttttcctgg caggcggtaa tggcttcgga
     1321 gacttggcgg cgggtttcgt tggtcttcat gaaagcgttg gtcgatctct ggtccttagg
     1381 atttgattgt aaatgacaat tgtctccacg gttcgagcgt agctgcgggc ttacggctta
     1441 ggtggccctt ttgtacagct cgcacttttt gatgtagtcc gccaccgccg ggtcaaccaa
     1501 tctgccaaga ggcttgccgg cagtgaccgc ttcacggatc gcagtcgccg agattgcatg
     1561 atgaacgccg tcgagcagat ggaccgtggc gccattcaga acaagatccc cccgcgctgg
     1621 ctgcttctcg aacggtttgg tgatatgagc tgcgggtcgc agtttttccg gaagcgagtt
     1681 cgcgacatcg gccagcgagt atcccgggcg ggacgccacg atgaattcac aaaggccaaa
     1741 tagctcttcg gcatcgtgcc acttcgctat gtcgcgaaac gcgtcaatgc cgatcaggaa
     1801 gaatatctca tcgatcttct tgagcgactg cttgagccgc cgcagcgtgt cgaccgtgta
     1861 gttggcgtca gggtgcttcg ccggtgcact cttcgaactg atcgcaactg ccttcgacgg
     1921 ccccggcaca tcttcaattg gcgcctccag cagcgacggc accagcgctt tgtccggagc
     1981 tgttgcgagc gcgaccatcg
//
LOCUS       DASTPM010000119         3130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1128225, whole genome shotgun sequence.
ACCESSION   DASTPM010000119 DASTPM010000000
VERSION     DASTPM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128225"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1340
                     /locus_tag="VFR42_02060"
     CDS             261..1340
                     /locus_tag="VFR42_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEU0367964.1"
                     /translation="MESSNLYASPCFVAATATALPPHVITRDDVKYYMGRVFEIPERR
                     LEAMMSIVDNAQVHQRHVIFPIDYTVEPRSLEKTNQEYMDHAIQLGQSAAEQCLERAG
                     MRADEIDLIITVSCTGFMIPSLDAHLINRMGFRSNVRRMPFTELGCAAGAMAMGRASD
                     YLQAYPGGNVLIISVELPSLTFQRKDISQANLISSILFGDGAAAVIISGKPNGRNPRI
                     LTSETYTFPDSLGAMGFDLKDSGFHILLSKDVPEMIGAKIEGLVDGFLSRHGQSRENI
                     RGWILHPGGSRLLGNVQEALGLNNCDTKPSWDILGSVGNLSSATILFILQEWLEKRPL
                     KKDEIAMAAAFGPGFSAEFLLLQWN"
     gene            1331..1915
                     /locus_tag="VFR42_02065"
     CDS             1331..1915
                     /locus_tag="VFR42_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0367965.1"
                     /translation="MELKMSLSVILFITLLLAVAVLRIFELRISKRHQQRMTSRGAAK
                     VKDPYFRWMAMFHTLVLIGAAGEVVFLNRPFIPALAAPMFVLFLAANVVRWWVIRTLG
                     EHWNVQVVDSTKLGFVASGPFRYVRHPNYAAVFVEMIALPLIHTAWITALAGAVAHII
                     VLSLRLSVEDPVLLSNPDYAATMGSKPRFLPGLF"
     gene            2024..2875
                     /locus_tag="VFR42_02070"
     CDS             2024..2875
                     /locus_tag="VFR42_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0367966.1"
                     /translation="MFKTDLLEGRAIFLSGGGSGLGRSMSIHFASLGAKLFVIGRREE
                     PLEETCQAIQQAGGTAAYATCDVRDFVAVEAAADKADSAVGPIDTVINNAAGNFMART
                     EKLSPNAFDTIVSIVLNGSFHCTQAFARRWIAAKKPGNVLSITTTYAAANCGSAFVVP
                     SSCAKAGVLAMTTSLAVEWAKYKIRLNAIAPGPFPTEGAWSRLVPSKEFDDRARESHP
                     MKRYGRHQELTNLAAFLISDMSEYINGECVVIDGAQWLRGAGEFNDLLELPESAWEQM
                     EAARSKK"
BASE COUNT          621 a         1020 c          775 g          714 t
ORIGIN      
        1 agcaacaatc caagcgcagt tctaatataa tcaaaacggt ggcggctcat ccccgggatt
       61 ctcctcatga ggcagccatc ttacagcgca aaaatcggat tttcgcggtt gtaaagaaaa
      121 aatcacacac gcttcgatga aagcgaaaca cgtatccata tcacgctttc actggcaaat
      181 ctctgcatct cgactcagaa tagggtagag tatcgagttc atctgcttct cacttcgcgc
      241 ccaggggact tttggacaga atggaatctt ccaaccttta tgcttcaccg tgttttgtcg
      301 cagccacggc caccgcgttg cctccccacg tcatcacccg cgatgacgtg aaatattaca
      361 tgggccgcgt gtttgagatt cccgagcggc ggctcgaagc catgatgtcc atcgtggaca
      421 atgctcaggt ccaccagcgc cacgtcattt tccccatcga ttacaccgtc gagccgcgct
      481 ccctcgaaaa aacaaatcag gagtacatgg accacgccat ccagctcggc cagagcgccg
      541 cggaacagtg cctggaacgc gccggaatgc gcgccgacga aatcgatttg atcatcaccg
      601 tgtcgtgcac tggcttcatg attccttcgc tcgacgcgca cctcatcaat cgcatgggtt
      661 ttcgttccaa tgtacgccgc atgccgttca ccgaactggg ctgtgcggct ggagccatgg
      721 ccatgggccg ggccagcgat tatctgcagg catatccagg cggaaacgtt ctgattatct
      781 ccgtcgagct accttcgctt acctttcagc gcaaagacat ttcgcaagcc aacttgatct
      841 cgtccatttt gtttggcgac ggagccgccg ccgtcatcat cagcggcaag ccgaacggac
      901 gcaatccccg catcctcact tctgaaacct acaccttccc cgattcgctg ggagcaatgg
      961 gctttgactt gaaggattcc ggtttccaca ttcttctttc caaagacgtc cccgaaatga
     1021 tcggagccaa aatcgaaggg ctggtagacg gctttctttc ccgccatggc cagtcgcgag
     1081 aaaacattcg cggctggatt ttgcatccgg gaggctcgcg cctgcttggc aacgtgcagg
     1141 aagcgcttgg cctgaacaac tgcgacacca aaccgtcctg ggacattctc ggcagcgtcg
     1201 gcaatctttc cagcgccacc attcttttca ttcttcagga atggctcgaa aagcggcctc
     1261 ttaaaaaaga cgaaatcgcc atggccgccg ccttcggtcc cggattcagc gcggaatttc
     1321 ttctcctgca atggaattga agatgtcgct gagcgtcatc ctgtttatta ctttgctgct
     1381 cgccgttgcc gtgctgcgca ttttcgaatt gcgcatttcc aagcgccacc agcagcgcat
     1441 gacttcccgc ggcgctgcca aggtcaagga tccctacttc cgctggatgg ccatgtttca
     1501 cacgctggtc ctgatcggag ctgccggcga agtggttttt cttaaccggc cattcattcc
     1561 cgcgctcgcc gctcccatgt ttgttctgtt cctcgccgcc aatgtggttc gctggtgggt
     1621 cattcgaacg ctcggcgaac actggaacgt ccaggtggtg gactccacca agctcggctt
     1681 cgtcgcctcc ggcccgtttc gctacgtgcg ccatcccaac tacgcggccg tttttgtgga
     1741 gatgatcgcg ctgccgctca tccacaccgc atggatcacc gccctggccg gcgccgtcgc
     1801 gcacatcatc gttctttcgc tgcgcctctc tgtggaagat ccggtgctgc tttcgaatcc
     1861 cgactacgcc gccaccatgg gctcgaagcc acgcttcctc cccggcttgt tctgattctt
     1921 aggcagcaca gccgctcttg gctgtgctgc gacgtaaggc cctgctgctg gcctataatg
     1981 cgttcgcact gtcgcattac atgcgctatc ggagcgtaaa ctcatgttca aaactgactt
     2041 gctcgaaggc cgcgccatct ttttgtccgg cggtggttcc ggcctcggcc gctccatgtc
     2101 cattcacttc gcgtctctcg gcgcaaagtt gtttgtcatt ggccgccgcg aagaaccgct
     2161 agaggaaact tgccaagcca tccagcaagc tggaggcacc gcggcctacg cgacgtgcga
     2221 tgtccgcgat ttcgtcgccg tggaagctgc tgcggacaaa gctgactccg ccgtcggccc
     2281 gatcgacacc gtcattaaca acgccgccgg caacttcatg gcccgtaccg aaaaactttc
     2341 tcccaacgcg ttcgacacta ttgtcagcat cgtgctcaac ggctcgttcc attgcacgca
     2401 agccttcgct cgccgctgga tcgctgccaa aaaaccgggc aacgtgctga gcatcaccac
     2461 cacgtacgcc gcggcgaatt gcggctccgc gttcgtggtt ccgtcttcct gcgccaaagc
     2521 cggcgtcctg gccatgacca cttccctcgc cgtcgaatgg gccaagtaca aaatccgcct
     2581 caacgccata gcgcccggcc cgtttcccac tgaaggcgct tggtcccgcc tcgttccttc
     2641 caaagaattc gacgatcgcg cgcgcgaatc tcatcccatg aagcgctacg gccgccacca
     2701 ggaactcacc aacctcgcgg catttctgat cagcgacatg tcggaataca tcaacggcga
     2761 atgcgtggtg attgatggcg cccaatggct gcgcggcgct ggcgaattca acgatctgct
     2821 cgaactgcca gaatccgcct gggagcagat ggaagccgcc cgctcaaaaa agtagcctga
     2881 attactttat gcgccaccgt gcggtggcca gtcttcagac cggctcgtat tgcttctctt
     2941 tgcaggtgcg cagctttcca caacaaattc ccgcactccc tgctactgtg ccaacgcgct
     3001 cgctttaagc tgaactttaa gccgcacttc accggtgctt tcgcctgccg ctgctccccg
     3061 cacaaccact tcctgcgcag caatcttttt caattcctct ttactcaact ccggcgtcgc
     3121 tacctctacg
//
LOCUS       DASTPM010000120         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_32663, whole genome shotgun sequence.
ACCESSION   DASTPM010000120 DASTPM010000000
VERSION     DASTPM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32663"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFR42_02075"
     CDS             complement(<1..654)
                     /locus_tag="VFR42_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367967.1"
                     /translation="MKILNGQLRLAATDVSNHLACRHLTQLDLQVARGERSAPQWAAP
                     DLKVIQELGRRHEAAYLKQLEEEKKLQVVRLLEHGADAELLAETLRLMAEGVDVIAQG
                     ALRDGEWFGRPDILLKVAMPSKNREWSYEVQDTKLARETKATTILQLSVYSELLEQAQ
                     GKAPECMWVITPAAGFAGERYRVAEYAAYFRYVKRELLKSGGANSGASGEKRSEGQLG
                     "
     gene            1458..2315
                     /locus_tag="VFR42_02080"
     CDS             1458..2315
                     /locus_tag="VFR42_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367968.1"
                     /translation="MALLLPFSAPYGALRAFPGSAGNPPPSFKPKTMEAFSAYVRATD
                     ARNNTELQNSKDFLWVDALPEAARNRAHKSLAAGESQIEQRSTLADGRAIPCPDGMIH
                     HWEGLIFIPGARVDDVLHVLEDYDRHSVYYAPDVARSRLEERDGDHFHAFLRFRRQKV
                     ITVVLNTSHDIRYFRDGPQRAHSRSSATHIAEVDNAGKTNEREKSRDDDNGFLWGMET
                     WWRLQEKDGGVYVQSEVVSLTRNIPTGVGWMIGPFITSIPKDTLSFTLEATRKAVLAQ
                     MKQNQTAAR"
     gene            complement(2326..3516)
                     /locus_tag="VFR42_02085"
     CDS             complement(2326..3516)
                     /locus_tag="VFR42_02085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0367969.1"
                     /translation="MRIAVISPFVDRRHGTERAVAELIERLTGKYGDEVDLYAQSVND
                     VGNLRRAGETASAGGKICWHQVGQFPGPLWLGFRGWLWLNRRARVREVRRSGKKPEVT
                     FSPGINALDADVILVHAVFHRLAELQTAHDHTGLRGLHRRLYYGMLCKLEQRIYSDPR
                     VTLAAVSKHTAEQLHRYFGRDDVAVIPNGVDVEHFSPAVIAPLRDAARKQRHCAPEQM
                     ALLLVGNDWRNKGLGTLLAAMANCKELPLRLLVVGQDELTPFRARAAGVLERVEFCPP
                     VADVRPLYAAADILVAPSLEDSFNLPVLEAMACGLPVIVSPEAGVSEWLAHGFDALLL
                     KDPENTNELADLIRVVASSSQLRRSLAQNAAATARKFSWDEHTAELRQLMGKAAAKKK
                     AAGP"
BASE COUNT          771 a         1327 c          915 g          782 t
ORIGIN      
        1 acccaactga ccctccgatc tcttttcgcc ggacgcgcca ctatttgcgc caccgctttt
       61 caacaactcc cgcttcacat accggaaata cgccgcatac tccgccacac gatatctttc
      121 tcctgcgaaa cccgccgccg gtgtgattac ccacatgcac tccggcgcct taccctgcgc
      181 ctgctccagc agctccgaat acactgacag ctgcagaatc gtcgtagcct tcgtctcccg
      241 cgccagcttc gtgtcctgca cctcataaga ccattcccgg ttcttgcttg gcatggcgac
      301 tttcagcaag atatccggcc gcccaaacca ttccccatcc cgcagcgccc cctgcgcaat
      361 cacatccacg ccttccgcca tcagccgcag cgtttccgcc agcaattccg catccgcccc
      421 gtgctccagc agccgcacca cctgcagctt cttctcctcc tccagctgtt tcaggtacgc
      481 cgcctcgtgc cgcctgccca attcctgaat caccttcaaa tccggcgccg cccactgcgg
      541 cgcggacctc tcccctcgcg ccacctgcaa atccagctgc gtcaaatgcc ggcaggccag
      601 atgattgctc acatccgtag ccgccagcct cagttgtcca ttgagaattt tcatggcgaa
      661 gagttaaagg ttaagactag gctactttta catccagtac cagcattgac tcgcttcgaa
      721 tttcatccga tctccctagc cgtccgggga gtcagccgag cagagcccgg tatctaagaa
      781 ctgttcctct ggccgctaga cgcgccttcg cagcgcgcag caacaggttg cgtctaggaa
      841 ctttacgcaa ccagcgcgaa aatttttgcg gctcacgaat ccccaacgcc gtgtgtcaag
      901 gcgctctgag ggggatcggt ttgaacccga tgcgaaacgt ccgccaataa catccgcaga
      961 atcagcaaga tactgtaatc aaataactcc tgtaaaatca acagcttact gtgacatcag
     1021 agcgcagcag gattcaaact atctcaaaac ataacgcagc gcttacctct ttcatgacgc
     1081 gcactagtta ctggctcgct cgtactaaaa ccccggtaac ctgtactttg gctccgttgg
     1141 cccattggtt gttttccgag cccgctttaa tgtggcacaa cgggttgggg gccgcgcggg
     1201 gacagcggct ccgcaaggag ccgtgtgcga gcttcccaac ccgctttctt ttctctcggc
     1261 cgacgggccg gctaaccggc cctgttatcc ttcggccatt cccctgcatc ccatattgtg
     1321 cggttccatt gcatatgctg caaatgaagg acttaccggc ttcccggcag cctcgaggcc
     1381 accgttttgg cgggtttagc cggcgctcgc ctcgccttta cctttctctt cttgcctccc
     1441 cgctcgctgc gcttctggtg gctctgttgc taccgttttc cgctccctac ggcgcgctcc
     1501 gcgcatttcc gggctccgcg ggcaacccgc cgccttcctt caaaccaaaa accatggaag
     1561 cgttctccgc ctatgtgcgc gccaccgacg cgcgcaacaa caccgaactg cagaacagca
     1621 aggactttct ttgggtcgac gcgcttcccg aagctgcccg caaccgcgcc cacaagtcgc
     1681 tcgcggcagg cgagtcccaa atcgagcagc gcagcaccct cgccgacggt cgcgctatcc
     1741 cctgcccgga cggcatgatt catcactggg aaggcctgat ctttattccc ggcgcccgtg
     1801 tcgacgatgt tttgcacgtt ctggaagact acgaccgtca ttccgtttac tacgcgcccg
     1861 atgtggctcg ctccagactc gaagagcgcg atggcgatca cttccacgcc tttctccgct
     1921 tccgccggca aaaagtcatc accgtcgtgc tcaacaccag ccacgacatt cgttattttc
     1981 gcgacggccc gcagcgggcg cacagccgca gcagcgccac ccacatcgcc gaagtcgaca
     2041 acgccggcaa aaccaacgaa cgcgaaaaat cacgcgacga cgacaacgga tttctctggg
     2101 ggatggaaac ctggtggcgc ctgcaggaaa aagatggtgg cgtctacgtg cagagcgaag
     2161 tggtttcgct gacgcgaaat attccgaccg gcgtcggctg gatgatcggc ccttttatta
     2221 cctctattcc gaaggacacg ctcagcttca cgctcgaagc cacccgaaaa gccgtcctcg
     2281 cgcaaatgaa acaaaaccag acggcagcgc gctaggcgag aacccttatg gccccgccgc
     2341 ttttttcttc gccgcggctt ttcccatcag ctggcgcaac tccgcggtat gttcatccca
     2401 ggaaaatttc cgcgcggtcg ccgccgcatt ttgcgctagg cttctgcgca actgcgaact
     2461 gctcgccacc acacggatca gatccgccaa ttcgttagta ttctccggat ctttcagcag
     2521 cagtgcgtcg aagccgtgcg ccagccattc gctcacgccc gcttccgggc tgacaatcac
     2581 cggcaagcca caagccatcg cctccagcac cggtaaatta aacgaatctt ccagcgacgg
     2641 agccaccaga atatccgccg ccgcgtacag cggacgcaca tccgcaaccg gcgggcaaaa
     2701 ttccacgcgc tccagcacgc ccgcggcccg cgcgcgaaat ggcgttagct cgtcctggcc
     2761 taccaccagc aatcgcaacg gcagctcttt gcaatttgcc atcgctgcaa gcaacgttcc
     2821 caatcccttg ttgcgccagt cgtttccgac caatagcagt gccatctgct ccggcgcgca
     2881 gtgccgttgc ttgcgcgccg catcacgcag cggagcgatc accgccggcg agaaatgttc
     2941 cacgtccaca ccgttcggaa tcaccgccac gtcgtcgcgg ccgaaatatc ggtgtagttg
     3001 ttccgcagta tgtttcgaaa cggccgccag ggtcacgcga ggatcgctat aaatccgctg
     3061 ttccaatttg caaagcatcc cgtagtaaag cctgcgatgc agcccgcgca gtccggtgtg
     3121 gtcgtgcgcg gtctgcagct ccgccagacg gtgaaacacc gcgtgcacca ggatcacgtc
     3181 ggcatccagc gcattgattc cgggagagaa agtcacctcc ggctttttac cgctccgccg
     3241 aacttctcgc acccgcgccc gccgattcag ccacagccat ccgcgaaatc ccagccacaa
     3301 cggcccgggg aattgtccca cctgatgcca gcaaattttg ccgccagcgc tcgctgtttc
     3361 gcccgcccgc cgcaaattcc cgacatcatt cacgctctgc gcgtaaagat ccacttcatc
     3421 gccatatttc cccgtcagcc gctcaatcaa ctccgccacg gcgcgttccg tcccgtgccg
     3481 ccggtccaca aacggagaaa ttaccgcaat ccgcaacttg ttcgcctcgc tccgacggct
     3541 tgtgcgcgcg ccaaggttca acatcgcgca aggtatcgtg aatcggtttc caattcaggc
     3601 tgacaaaagc ttatagtctg cgttggttat ttcaaaagca tacgaaaagc tgaaactgca
     3661 ttcctggcaa ggcacaggtg cccgggccct aacgcagtgc aggcactaac ttaggcaggg
     3721 tctactctga tcgtccgcgc tgcctccatt gatttaatat ccgcctctat tatcgagtat
     3781 caccaccgca gctgg
//
LOCUS       DASTPM010000121         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_292210, whole genome shotgun sequence.
ACCESSION   DASTPM010000121 DASTPM010000000
VERSION     DASTPM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292210"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFR42_02090"
     CDS             complement(<1..1326)
                     /locus_tag="VFR42_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEU0367970.1"
                     /translation="MPRNCKRSLRITEFYKPFPRQEQFHGSSAKYRLFGGAAGPGKTK
                     ALLWEAIRQAQRVPRSDSLLLRRTYPELESSLLAYFRRDVPRKSYRSYNEAKHIVTWP
                     NGSTTRFGYCRNENDVYQYQGAEFLFIGLDELTHFTLKQWQFLTSRNRCPIPGSRTSM
                     AGASNPGNIGHAWVKALWVDKKPPAGFDRPELYDPGEYDFIRAKLADNPIYANDAAYH
                     RTLEALPEHLRRAFLDGDWNVFAGQYFDVFEFGRHTMRAESLRLEPWWPRWISIDWGF
                     QHPSAVYWHCAVPVTGDWRAVTGENLSSQPGFHQSAVTSHPSASRIVTYREFVQSGLS
                     PRMLAQAIAERSGRERISDVFLSPDAFAHRTSEASIAEQLGEVLTATGLPRPAPADDD
                     RIGGWQRMYQLLESDAWLITENCAKLIDCIPQLVRDAARVEDIRKVDGDD"
     gene            complement(1336..1515)
                     /locus_tag="VFR42_02095"
     CDS             complement(1336..1515)
                     /locus_tag="VFR42_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367971.1"
                     /translation="MEKLTQAIYLDRVLKIAGVAGFVVTYLNAWRSMHLGLKIGFICS
                     AAAWFVGARFTKIYR"
     gene            complement(1542..2093)
                     /locus_tag="VFR42_02100"
     CDS             complement(1542..2093)
                     /locus_tag="VFR42_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367972.1"
                     /translation="MNARSKPNGQGALLAKGSGANGHTISLSELDRAASVDASELSVA
                     NAAASSVVAGAEREAHAEVARSGGNGKRPGSAPAASRSHHRNDAEEQGSVSPAKKAKR
                     KIPPGTEALPVDGEAFVDAVHSRVDLVLLEEKLLNSKDEKVAQRELAYLRELRYGKRA
                     PSGDDGPQQLIDDIPRPEEDNTP"
     gene            2648..3001
                     /locus_tag="VFR42_02105"
     CDS             2648..3001
                     /locus_tag="VFR42_02105"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU0367973.1"
                     /translation="MPHYLSQVSYTPEAWARLVAHPQDRIEAVRGPIEKLGGRIHNAF
                     FAFGEYDVVVITEMPDNVSAAAIAIAFAAGGAVKNEKTTPLMTTAEALEAIKKAGTTG
                     YRSVTASASAASARP"
BASE COUNT          616 a         1006 c          849 g          615 t
ORIGIN      
        1 atcgtcgccg tctaccttgc gaatgtcttc cacgcgcgcg gcatcgcgca ccagttgcgg
       61 aatgcaatcg atcaatttcg cgcaattttc cgtgatcagc caggcatccg actccagcag
      121 ctgatacatc cgctgccagc cgccgatgcg atcgtcatcc gcgggagccg gccgcggcaa
      181 ccctgtcgcc gtgagcactt cgccgagttg ctcggcgatg gacgcttctg aagtgcgatg
      241 cgcaaacgcg tccggcgaga gaaacacgtc gctaatgcgc tcgcgcccgc tgcgctccgc
      301 aatcgcctgc gccagcatgc gcggcgaaag tccgctctgc acgaattcgc ggtaagtgac
      361 gatgcgagaa gctgatgggt gacttgtcac tgctgattgg tgaaacccgg gctgagatga
      421 aaggttctcc ccagtcaccg ctctccagtc accagtcact ggtactgcgc agtgccaata
      481 aaccgcgctc ggatgctgaa agccccagtc gatcgaaatc caccgcggcc accatggctc
      541 gagccgcaac gactcggcgc gcatcgtgtg gcggccgaac tcgaagacat cgaagtattg
      601 cccggcaaaa acgttccagt cgccgtccag aaacgcgcgc cgcaaatgct ccggcaaggc
      661 ctcgagcgtt cggtgatacg cggcgtcatt cgcgtaaatt ggattgtcgg ccagcttcgc
      721 gcgaatgaaa tcgtactcgc ccgggtcata cagttccgga cgatcgaagc ctgccggcgg
      781 cttcttatcg acccacagcg ccttcaccca cgcgtgcccg atgttgccgg gattgctggc
      841 tccggccatg ctcgtgcggc tgcccggtat cggacagcga ttgcgcgaag tcagaaattg
      901 ccactgcttc agcgtgaagt gtgtcagctc atcgagcccg atgaacagaa actccgcgcc
      961 ttgatactgg tagacatcat tttcgttgcg gcaataacca aaccgcgtgg tcgagccgtt
     1021 cggccacgtt acgatgtgct tcgcttcgtt gtagctgcga tacgatttgc gcggcacgtc
     1081 gcgccgaaaa tatgccagca acgacgactc cagctccgga tacgtgcggc ggagcaacag
     1141 cgagtccgac cgcggcaccc gctgcgcctg gcgaatggct tcccacagca gggctttggt
     1201 tttccccggc cccgccgcgc caccaaacag ccggtacttc gcggagctgc cgtggaactg
     1261 ttcctggcgc ggaaacggct tgtaaaactc ggtgatgcgc aacgaacgtt tgcaatttcg
     1321 cggcacgctt cacgcttagc gataaatttt ggtaaagcgc gcgcccacaa accacgccgc
     1381 cgcgctgcag atgaaaccga tcttcaggcc caggtgcatg ctgcgccaag cattcagata
     1441 ggtcaccacg aagcccgcta ctcccgctat cttcagcaca cgatcgagat agatggcttg
     1501 ggtcagtttt tccaacagac ttcgcgaagc actcaataca ctcatggcgt gttgtcctct
     1561 tccggccgcg gtatgtcgtc gatcagttgc tgcggcccgt cgtcaccgct gggcgcgcgc
     1621 ttgccgtagc gcagctcgcg caagtaagcc agctcccgct gcgccacctt ttcgtctttg
     1681 gagttcagca gcttttcttc cagcaaaacc aaatccacgc gactgtgcac ggcgtccacg
     1741 aaggcctccc catcgacagg aagagcctcg gtcccaggcg gtatttttcg ttttgctttt
     1801 ttagcagggg atacgcttcc ctgctcttca gcgtcgttcc ggtgatggga ccgggaagca
     1861 gccggcgccg accccggccg cttcccatta cccccggacc gagccacctc agcgtgcgcc
     1921 tcgcgttccg cgccagccac gacggaggat gccgcggcat ttgcaacgga gagttcgctt
     1981 gcgtcgacag acgcagcccg gtcgagttcg ctcaacgaaa tcgtgtgacc attcgcgccg
     2041 ctgcccttgg ccagcaacgc gccctgccca ttcggtttgc ttcttgcatt catgatttca
     2101 gttcactcgc tgcgttaaag cagattgcgg tctcgccgct tgcggccctt tcggatcgtg
     2161 cgggaacccg tgcgtccaca gccccagcaa cgcgccgtgc agcgaatcca ggtgcctgcg
     2221 gcagagtgga tgcccttgcg tacacgcccg tcgacacctc acccaggcat ttcgctgaac
     2281 ccgcgaaacg cgatatgcaa cacactgttt caaagttggt gcctgtgggt tagtgcccgg
     2341 atttctaggg cttacgttgg tgtagcgagc gcgccttaaa acaaaaacag cccccggcaa
     2401 accggaggct gtgcgagaaa tctgcgtccc gaaaagcaaa acgccccgtt tgcgtcgggg
     2461 cgtagtttct tacaagagag agtgtatacc gatttgtact tttttgtcaa gggaaatctt
     2521 caattatttt tgtttttcct tgaacccatt tcttttcagg agcatagttc cccaattccc
     2581 cagccaaatt ttaacttgcg cggtcccgct tccacccgtc ggtgccacgc aagccggagg
     2641 aggaaacatg ccgcactatc taagccaagt gagctacacg cccgaagcct gggcgcgtct
     2701 cgttgctcat ccccaggacc gcatcgaagc cgtgcgcggc ccaatcgaaa aactcggcgg
     2761 ccgtattcac aacgcgttct tcgcgttcgg cgaatacgat gtcgtcgtca tcaccgaaat
     2821 gcccgataac gtcagcgccg cggccatcgc catcgccttt gccgccggcg gcgcggtgaa
     2881 aaacgaaaag accaccccgc taatgaccac cgccgaagcc ctcgaggcca tcaagaaggc
     2941 cggcacgacc ggctatcggt ctgtcacagc atccgcaagc gccgcatccg cccgcccgtg
     3001 agcgtaacca gataggtcga tccgccaggc aggggcgcat cctgttgcgc ccccgccacc
     3061 aaccgcggaa agctcatggc taaaat
//
LOCUS       DASTPM010000122         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_782536, whole genome shotgun sequence.
ACCESSION   DASTPM010000122 DASTPM010000000
VERSION     DASTPM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782536"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /gene="menC"
                     /locus_tag="VFR42_02110"
     CDS             complement(<1..1024)
                     /gene="menC"
                     /locus_tag="VFR42_02110"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEU0367974.1"
                     /translation="MRVEQLTLRELHMKLVAPFETSMETTSLRRVVLVEAVVDGVTGW
                     GECVAGETPSYSPETTETAWHIVRDHLWPTLKGKQFASAGDVSDLFGWVRGHNIAKAG
                     LEAAVWDAEAKQKQIPLAKLLGGTRGEISSGVSIGIKASLDELVRAVEVELEAGYQRI
                     KIKIKPGCDLEAVRVLRTKFPAIKLMVDANSAYTLGDASLLKKLDEFYLMMIEQPLGW
                     DDLYSHVELQRELKTPICLDECIHAEEQAVAAIRLGACRIINIKLGRVGGYTEARRIH
                     DLCQGNGVPVWCGGMLESGIGRTHNIALSTLPNFTLPGDVTASKRYWAEDIIEPEVTV
                     SRHGTIR"
     gene            complement(1045..1782)
                     /locus_tag="VFR42_02115"
     CDS             complement(1045..1782)
                     /locus_tag="VFR42_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367975.1"
                     /translation="MSGEIVVRSCRGLDEFRACVALQKEIWNEADLEIVPDTVFVVAL
                     HTGGQILGAFDGERLIGFTLSMPGLRNGAPYLHSHMTGVRAEYRDRGAGRMLKLLQRD
                     EALSRDIRLIEWTFDPLEMRNAHFNLNRLGAIARRYEPNLYGITSSPLHRGLATDRLV
                     AEWYLDSPRALAAMNGELPPPQFAESIEIPATLEDWKASDLPQVQALQSRIREQFMEG
                     FAKGYAAVGVARTARGTAYQLAPWSEF"
     gene            complement(1779..2717)
                     /gene="mazG"
                     /locus_tag="VFR42_02120"
     CDS             complement(1779..2717)
                     /gene="mazG"
                     /locus_tag="VFR42_02120"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU0367976.1"
                     /translation="MARPKKQPAKAEKRAARSAQLRNESAGAWFERLVRLQARLRAPK
                     GCPWDIEQTHLSLRTYLIEEAYEVLEALESGDDGKFAEELGDLLLQVVFHAQIATEQG
                     RFTAADVVREIYQKMVRRHPHVFGEKSARNAADVLKNWEQIKAEERRAKGAEKGSGKA
                     SADQEPASLLDGIPHTLPAVMQGFQLTRRASRIGFDWERVEGVVEKLQEELGELSQAR
                     ETRSAQRVEEEIGDILFAAVNLARFLKVDPEIALKRTNAKFSARFREMERVAAASGRK
                     LADVARDEMEALWDRAKASEKAGAQAYRARKAAGKR"
     gene            2862..3356
                     /locus_tag="VFR42_02125"
     CDS             2862..3356
                     /locus_tag="VFR42_02125"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEU0367977.1"
                     /translation="MPEQKPEESFRVIDRRPFTNEGELRQEIVDEQEREARQEAKKEA
                     TAAPHPAPAAAGDGKTPPSADTPKRSPAFENLIRMLGSNAAMVLGGYADPRTGQPVID
                     PDAARELIDMLDALHEKTKGNLAPEEDSLLLDLNGKLKLTYLEINQALAAQAAAQTGK
                     AKAR"
     gene            3407..5233
                     /locus_tag="VFR42_02130"
     CDS             3407..5233
                     /locus_tag="VFR42_02130"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU0367978.1"
                     /translation="MSATATPLQLTILGSGTSMGVPTIGCRCAVCTSTDPRDNRMRTS
                     VLLSRNGQHVLIDTTPDFRSQALRAGIQRLEAVLLTHGHADHVMGFDDLRPLSIGQKR
                     PMPVYANAQTFDIVRRAFSYAFDGKPKVSSVPSVLLKEINGPFELLGVQITPVPLEHG
                     EMQVLGFRFGKGAYLTDFNAVPESSLALLRDLDDLVLDALRDTPHPMHQTVEQALALV
                     AKLKPQRAWFTHIAHDLGHAETNERLDRLGHPNVQLAYDGLQLEIATQASARARSGLE
                     VLDSRVAGLAVFDSAEKWREFYGPSGRRSVLAIGNFDGIHLGHQAILKQAVELAAKTA
                     AVATALTFEPSPLKVLRPESAPKRISTPEQRLEWFRAVDVESAVVLPFTIELSKLTPQ
                     EFIERILVRELQVRAVLVGENFRFGHKQAGDTTLLRELGRVHGFEVIAIAPVAAHGEI
                     VSSTVIRREIAAGDVTQAGRLLGRPYVLTGEIVSGTGTGRKFTFPTLNLKPDQELLPA
                     PGVYITRTLLAGEPKSRRSVTNIGTRPTFSGTALSVETHLLNFSCAIAPSRIELRFWK
                     RLRSERKFPAPEELRAQIGRDIRSAERFFTILRKFRVLRLSD"
BASE COUNT         1064 a         1831 c         1415 g         1090 t
ORIGIN      
        1 ctcgaatcgt gccgtggcga gagacggtga cctccggctc gatgatgtct tccgcccagt
       61 aacgcttgct cgccgtaacg tcgcccggca gcgtgaagtt tggcaacgtg gaaagcgcga
      121 tgttatgcgt ccggccgata cccgactcca gcattccgcc gcaccacacc ggcacgccgt
      181 ttccctgaca taagtcatgg atgcgccgcg cctcggtgta ccctcccacc cggcccagtt
      241 tgatattgat gatgcggcag gcgccgagcc gaatcgcggc caccgcttgc tcctcggcgt
      301 gaatacactc gtcgagacag ataggcgttt tcaactccct ctgcagctcc acatgcgagt
      361 agagatcgtc ccaaccgagc ggctgctcga tcatcatcag gtagaactcg tccagttttt
      421 tcaacagcga agcgtcaccc agcgtatatg ccgaattcgc atccaccatg agcttaatcg
      481 ccggaaattt cgtacgcagc actcgaaccg cttccaggtc gcagcccggc ttaatcttta
      541 tcttgatgcg ctgatatcca gcttcgagtt ccacttcaac tgcgcgcacc agctcgtcaa
      601 gtgaagcttt aattccgatt gatactccgc tggaaatctc tccccgtgtt ccgccaagta
      661 gcttcgccaa cggaatctgt ttttgcttcg cctccgcgtc ccaaaccgcc gcttcgagcc
      721 ccgccttggc tatgttgtgc ccacggaccc agccgaacaa atccgagacg tcgccggccg
      781 acgcaaactg cttgcccttc agggtgggcc acaaatgatc gcgaacgatg tgccaagccg
      841 tctcggtagt ctccgggctg taggaaggcg tctcgccggc cacgcattcg ccccagcccg
      901 tgactccgtc caccacggcc tcgaccagca ctacccggcg tagcgacgta gtctccatgc
      961 tggtctcgaa cggtgcgacc agcttcatgt gcagttcgcg aagtgtgagt tgttctacgc
     1021 gcataaatgg ccgcagggca ggtgctaaaa ctcgctccat ggcgccaatt gataggcggt
     1081 cccccgggca gtcctggcga cgccgacggc ggcgtagccc ttggcaaaac cctccataaa
     1141 ctgctcgcga atcctgctct gcagcgcttg cacttggggc aggtcgctgg ccttccagtc
     1201 ttccagggtc gccggaattt cgatcgactc ggcaaactgg ggaggcggca actcaccatt
     1261 cattgccgcc agcgcgcgcg gcgaatccag ataccactcc gccaccagac ggtccgtggc
     1321 aagtccgcgg tggagcgggc tgctggtaat gccatacaga ttcggttcgt agcggcgcgc
     1381 gatggcgccc aatcggttca ggttgaaatg tgcgttgcgc atttccagcg gatcgaaggt
     1441 ccactcaatc agtcgaatat cgcggctcag cgcttcgtcg cgctggagca gcttcaacat
     1501 gcggcccgcg ccgcggtcgc gatactccgc ccgaacacca gtcatgtgcg aatgcagata
     1561 gggcgcgcca ttgcgcagac ccggcatgga caaggtaaac ccgatcaatc gctcgccgtc
     1621 aaacgcgccg agtatctgcc cacccgtgtg caacgccact acaaagacgg tgtccggcac
     1681 aatctccaga tcggcctcgt tccagatctc tttttgcagc gcgacgcagg cgcgaaattc
     1741 gtccaggccc cggcaggagc gcactacaat ctcgccgctc atcgcttgcc cgccgctttt
     1801 cgcgcacggt aagcctgcgc accggctttt tcagaagctt tggcccgatc ccaaagcgct
     1861 tccatttcgt cgcgcgctac atccgccagc ttccgtcccg aggccgccgc cacccgctcc
     1921 atttctcgaa aacgcgcaga aaacttggcg ttggtgcgtt tcagcgcaat ctccggatcc
     1981 actttcagga atcgcgccag attcaccgcc gcaaaaagaa tgtcgccgat ctcttcctct
     2041 acccgctgcg ccgaccgcgt ctcgcgcgcc tggctcagct ctcccagctc ttcctgcaac
     2101 ttttcgacga cgccctccac cctctcccaa tcgaagccga tgcgcgacgc cctacgtgtc
     2161 aattgaaatc cctgcatcac cgccggcagg gtatgcggaa tgccgtcgag aagcgaagca
     2221 ggctcctggt ccgccgaagc tttgccgctt cccttctctg cacccttggc gcgccgctcc
     2281 tcggccttga tctgctccca gtttttcagt acgtccgcgg cgttgcgggc gctcttttcg
     2341 ccgaacacgt gcggatgccg ccgcaccatt ttttggtaaa tttcgcgtac cacatccgct
     2401 gcggtgaagc gaccttgttc cgtggcgatc tgcgcgtgga acaccacctg cagaagcaaa
     2461 tcgcccaatt cttccgcgaa cttgccgtcg tcaccgctct ccagcgcctc cagcacctcg
     2521 tacgcttctt cgatcagata ggtgcgaagc gaaagatggg tctgctcgat gtcccaggga
     2581 caccccttgg gcgcacgcaa gcgcgcctgc aatctaacca gtcgctcgaa ccacgcgcca
     2641 gcggattcgt tgcgtagttg cgcactccta gccgctcttt tttctgcttt cgccggttgc
     2701 ttttttggtc tggccatcgg tcctggctgc tatttcgctg catcgaaact ccacactgta
     2761 aacagcgcac ctcattttat caagccatgc accatacgac cgaagcagct ttcgccgcta
     2821 gccgtgctaa acttgtaaaa aatacgaatt gaggtcgcac cgtgcctgag cagaaaccag
     2881 aagagtcgtt ccgcgtaatt gaccgccgcc cgtttaccaa cgagggcgaa ttacgccaag
     2941 aaatcgtcga cgaacaggag cgcgaagccc gccaggaagc caaaaaggaa gccacagcag
     3001 ctccacaccc tgccccggca gccgctggtg acggaaagac gccgccctcc gcggacactc
     3061 ccaagcgttc gccggcgttt gaaaatctga ttcgcatgct gggcagcaac gcagcgatgg
     3121 tactcggcgg ttacgccgat ccgcgcaccg gccagccggt catcgatccc gacgccgctc
     3181 gcgaattaat tgacatgctc gacgcgctgc acgaaaagac caagggcaat ctcgcgcctg
     3241 aagaagacag cctgctgctt gacttgaacg gcaaactgaa gctgacctat ctggagatca
     3301 accaggcgct ggccgcgcag gccgcagcgc aaaccggcaa agccaaagct cgctaggact
     3361 cgctctgcgc cgtgtagcat aggtcggtcg ctaatttctt tcgataatgt ccgccaccgc
     3421 cactccgctg caattgacca ttctcggctc tggcacctcc atgggagttc cgaccatcgg
     3481 gtgtcgctgc gcggtctgca catcgaccga tccgcgcgac aaccgcatgc gcacttcggt
     3541 tctgctttca cgtaacggcc aacacgtctt gattgacacg acgccggact tccgctcaca
     3601 ggctttgcgc gcgggaattc aacgcctcga agccgtactt ttgacgcacg gccacgccga
     3661 tcacgtgatg ggtttcgacg acctgcgtcc gctgagcatc ggccagaagc gccccatgcc
     3721 cgtgtatgcg aatgcgcaaa cgttcgacat cgttcgccgc gcattttcct acgcgttcga
     3781 cggcaagccc aaggtcagca gtgtgcccag cgtcctgctc aaagaaatca atggcccgtt
     3841 cgaattgctc ggcgtccaga tcacaccagt ccctctcgag catggcgaaa tgcaagtgct
     3901 cggcttccgc ttcggcaaag gcgcctacct caccgacttt aacgcagtgc ccgaatcttc
     3961 tctggcactt ctgcgagatt tggatgattt ggtcctcgat gcgctgcgcg atacgccgca
     4021 tcctatgcat cagaccgtcg aacaagcatt ggctctggtg gcaaagctca aaccccagcg
     4081 cgcgtggttc acgcacattg cgcacgacct gggtcacgcg gaaaccaacg agcgactcga
     4141 ccgcctgggg caccccaacg ttcaactggc ttatgacggc ttgcaactcg aaatcgccac
     4201 gcaagcctcc gcacgcgctc gttccggtct cgaggttctc gattctcgcg tcgcaggcct
     4261 tgccgttttc gattctgctg aaaagtggcg cgagttttat ggccccagcg gccgccgcag
     4321 cgtgctggct attggcaact tcgacggcat tcatctgggc catcaagcca tcctgaaaca
     4381 ggcagtcgag ctcgcagcga agaccgccgc ggtagcgaca gccctcactt tcgagccctc
     4441 tccgctgaaa gtcctgcgcc cggagagcgc ccccaagcgc atctccacgc ccgagcagcg
     4501 cctggagtgg ttccgcgccg tggacgtcga atccgccgtc gtacttccct tcaccatcga
     4561 actctccaag cttacgccgc aagaattcat cgaacgcatc ctcgtccgcg agctgcaggt
     4621 gcgtgccgtt ctggtaggag aaaatttccg cttcggccac aaacaggccg gcgacaccac
     4681 attgctacgc gaactcggcc gcgttcatgg cttcgaagtc atcgccatcg ctccggtcgc
     4741 cgcgcacggt gaaattgtat ccagcaccgt cattcgtcgc gagattgcgg caggagacgt
     4801 cacccaagct ggacgtttgc tcggccgccc ctatgtacta accggcgaaa tcgtcagcgg
     4861 taccggcacc ggccgcaaat tcacttttcc aactttgaat ctgaaacccg accaagaatt
     4921 gctccccgct ccgggcgtct acatcacgcg cacactgctt gcaggcgaac ccaagagccg
     4981 ccgttccgtg acgaacatcg gcacacgtcc cacctttagc ggcaccgccc tgagcgtcga
     5041 aacccacttg ctgaatttta gttgtgcgat tgcgcccagc cgaatcgagc tgcgcttctg
     5101 gaagcgcctg cgttctgagc gaaaattccc cgctcccgaa gaacttcgcg cgcagattgg
     5161 ccgcgatatc cgttcagccg aacgcttctt cacaatcctg cgcaaattcc gcgttctgcg
     5221 gctttcggac taatcttccg ggcgcgtaca aggaaagcaa gcgcgaactc cgagcggcag
     5281 actttgcggc tcaatgccac atcgttcgcg aatcccaact cgagccatca ccttgctgtt
     5341 gcgtactcgc cacgttcgtc ggcatccagt tttccagcgc acgttccagc agcggcgaaa
//
LOCUS       DASTPM010000123         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_90574, whole genome shotgun sequence.
ACCESSION   DASTPM010000123 DASTPM010000000
VERSION     DASTPM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90574"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFR42_02135"
     CDS             <1..432
                     /locus_tag="VFR42_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367979.1"
                     /translation="HNVVLDIHTMIFGVIFALLGAQILAIGAFAKVFSYAERFDRNTV
                     SLKRVLKRVSLEKGLLLGGVLFAAGFLGCAYIAWQWAASGFGPLAQVRGVLFWSMWLF
                     LGVQIIFSSFFLSMLGISRGTYIGDYDLSAEERNASEAAGK"
     gene            433..990
                     /locus_tag="VFR42_02140"
     CDS             433..990
                     /locus_tag="VFR42_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0367980.1"
                     /translation="MAYTQLDHFIAKMRFRAAYPHLKPGSRVCDLGCGLEMAFLDFAE
                     DKIARGVGVDDQVESGTHGRWQRVHADLRAKLPFADGEFDHVVMLAVFEHLVQPEAVL
                     REAFRILTPGGSLVMTWPAALVDPILNVMHGFHLISDEMESDEHQKRLPVSEVLRMAE
                     RVGFTETFHRTFEMGLNNLLVAARS"
     gene            complement(1047..2756)
                     /locus_tag="VFR42_02145"
     CDS             complement(1047..2756)
                     /locus_tag="VFR42_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367981.1"
                     /translation="MICPDPPDLEPLKQTFYYSLIGAGILMALQFAFAKPAARISRVA
                     WQWLGAIGRRPGLCACGLFVAVVSVRVALLHRLPIPVGGIHDEFSYLLMGDTFVHGRL
                     SNPPHPMWMSLEAFHVNFFPRYASMYPPLQGVVLAVGQVLGNPWIGVVLSCGVMVAVM
                     YWMLLAWVPSRWALLGSVFVWLKFCVTSYWINSYWGGAVGATGGALLLGALARIVQKP
                     RIRDAAAFALGLAILANSRPYEGFLFSLPAGIYSLYRLYRSGARCFPTTSRWKIVLLP
                     MLAVLIPTAAAMAIYNHNLTGHVLVFPETLNVRTYHTAPMFLFQAAKPPLQYHNQQFE
                     EFYNGWEREEYDHTWESVKCLTWLKTVRFFSAFAWWGMLLVAPGIYWVLRNRRLRLLW
                     IMLALVGAGAYLVVWSNAHYASPATCIVILLYLQSLRYVRRMRGKRWRWGAILARSSV
                     LLLLADTTTAVVRKQCDTIYWTCQGDVSRLVVQRKLEALPGKHLVMVRYGEDHNIHDD
                     WVFNGADIDSQRVVWAREIDENTDAKLFDYFRDRKAWLVTPDDDNTYLAPYIPPPDRA
                     QEK"
     gene            complement(2845..>3359)
                     /locus_tag="VFR42_02150"
     CDS             complement(2845..>3359)
                     /locus_tag="VFR42_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU0367982.1"
                     /translation="MRDRETIEIALEASETGHLVLSTLHTIDAAKTVERIVGVFPLAD
                     QQAIRTRLGKAFRFIVSQRLLPRKDGNGRIAAVEVLKSTMRTRDYVEKGESEGKSLLD
                     AMRDGSNEGMQHFDAELSKYVREGLVNLDVAMGFATNAGNFRLEMQDYLDDPQNRRPK
                     APGTDPDLEP"
BASE COUNT          721 a         1028 c          932 g          678 t
ORIGIN      
        1 cacaacgtcg tgctcgacat tcacaccatg atcttcggtg tcattttcgc tctgctaggc
       61 gcgcaaattt tggccatagg cgcattcgcc aaagtcttca gctatgcgga gcgttttgac
      121 cgcaacacgg tttccctcaa gcgtgtattg aagcgcgtct ccctcgaaaa ggggttgctc
      181 ctcggcggcg tgctcttcgc ggctggcttc ctaggctgcg cctacatcgc ctggcaatgg
      241 gctgccagcg gattcggtcc gctcgcgcaa gtccgcggcg tcctgttctg gtccatgtgg
      301 ctgtttctcg gcgtgcagat tatcttttcc tcctttttcc tgagtatgct gggcatcagc
      361 cgcggcacct acatcggcga ctacgatctc tctgccgaag agagaaacgc ttccgaggcc
      421 gccggcaaat aaatggccta cacgcaactg gaccacttca tcgcaaaaat gaggtttcgc
      481 gcggcgtatc cgcatctgaa gccgggttcg cgtgtgtgcg acctgggctg cggcctggaa
      541 atggcctttc tggatttcgc ggaggacaaa atcgcgcgcg gcgttggcgt ggatgaccag
      601 gtggagagcg gaacccatgg ccgctggcag cgtgttcacg cagacctgcg cgccaagctg
      661 ccgtttgccg atggcgagtt cgatcatgtg gtgatgctcg ccgtcttcga gcacctagtg
      721 cagccggaag cggtgctgcg cgaagccttc cgcatcctca cgccgggtgg ctcgctcgtc
      781 atgacctggc ccgccgctct ggtcgatccc atcctcaacg tcatgcacgg atttcatctc
      841 atcagcgacg aaatggaaag cgacgagcac cagaagcgcc tgcccgtcag cgaagtctta
      901 agaatggccg agcgagtggg cttcaccgaa acgtttcacc ggacttttga gatgggattg
      961 aacaatctgc tggtcgcggc gagaagttga ctgtggaccg cctcatgtcg aacgacaagt
     1021 gatcggcatc gcgacgattt gatcgttcat ttttcctggg cgcgatcggg tggcggtatg
     1081 tagggtgcca ggtacgtgtt gtcgtcatcc ggcgtaacca accaagcctt ccggtcgcgg
     1141 aaataatcga agagctttgc gtccgtgttt tcatcgattt cacgagccca aactactctt
     1201 tgcgagtcga tgtccgcgcc attgaaaacc cagtcgtcat gaatgttgtg gtcttcgccg
     1261 taccgcacca tcaccagatg cttgccgggc agcgcttcga gtttgcgctg aaccaccagc
     1321 cggctgacat cgccctggca agtccagtaa atcgtgtcgc actgtttgcg caccactgcc
     1381 gtggtggtgt ccgccagcag caacagcacc gagctgcgcg ccaggatggc tccccagcgc
     1441 cagcgcttcc cgcgcatccg gcgtacatag cgcagcgact gcaaatagag aaggatcaca
     1501 atacaggtgg cgggcgaggc atagtgggca ttggaccaca ccaccaggta agccccagcc
     1561 ccgaccagtg ccagcatgat ccacagcagc cgcagccgac gattgcgcag aacccaataa
     1621 attcctggcg cgaccagcag cattccccac cacgcgaacg ccgaaaagaa gcgcaccgtt
     1681 tttagccacg tcaggcactt cacgctttcc catgtgtggt cgtactcttc gcgttcccag
     1741 ccgttgtaaa actcctcgaa ttgttgattg tgatattgca gcggtggctt ggcagcctgg
     1801 aaaagaaaca tcggcgcggt gtgatacgtc cgaacgttga gcgtttccgg aaaaaccagc
     1861 acgtggcccg tcaggttgtg gttatagatg gccattgccg ctgcggtcgg tatcagcacg
     1921 gcgagcatcg gaagaaggac aatcttccat cgtgaagtag ttggaaaaca ccgcgctcct
     1981 gatcggtaaa gccggtagag tgaatagatc ccggctggca aactaaacag aaaaccctcg
     2041 taaggccggc tgttcgcaag aattgccaga cccaaggcga acgccgccgc gtcgcgaatc
     2101 cgtggtttct gaacaatccg ggccagcgct cccagcaaga gggcgccgcc cgttgcaccc
     2161 acagcccctc cccaatagct gttgatccag tagctcgtca cacaaaattt gagccaaaca
     2221 aaaacgctgc ccagcagcgc ccagcgcgaa ggcacccatg ccagcagcat ccaatacatc
     2281 actgccacca tcacgccaca gctcagcacc acgccgatcc acggattccc caaaacttgg
     2341 ccaaccgcaa gcactactcc ctgcaacggc ggatacatcg acgcgtagcg gggaaaaaaa
     2401 ttgacgtgga aagcttccag gctcatccac atcgggtgcg gcggattcga cagacgtcca
     2461 tgcacgaacg tgtcgcccat cagcaaataa ctgaattcgt cgtggatccc gccgacagga
     2521 atgggcaagc ggtgcagcag cgcaacgcga acgcttacca ccgcaacgaa gagcccgcac
     2581 gcgcataagc ctggccggcg tcctatggct cctagccatt gccatgccac ccgtgaaatc
     2641 cgcgccgctg gcttggcaaa cgcgaattgc agcgccataa ggatgccggc gccgatcagc
     2701 gagtagtaga aggtttgctt tagcggttct agatctggcg gatcaggaca aatcatcgcc
     2761 aagaatcata caggaagctc tgcgcgtcgt ttaaggcgct actacagcca gaatccagtg
     2821 taaaagagag cgcttaaagt gccctcaggg ttccaggtca gggtcggtac ccggtgcctt
     2881 cgggcgacga ttttgcggat cgtcgaggta gtcctgcatt tccagccgga aatttccggc
     2941 gttggtcgca aagcccatgg ccacgtccaa gttcaccagg ccctcgcgga catatttgct
     3001 caattcagcg tcgaaatgct gcataccctc atttgaaccg tcgcgcatgg catccagcag
     3061 cgacttgcct tcactctcgc ccttttccac gtagtcgcgc gtgcgcatcg tggacttcaa
     3121 aacttccact gctgcgattc gcccgttgcc gtccttgcgc ggcagcaagc gctgcgaaac
     3181 gataaaacga aacgccttgc ccagccgcgt ccggatcgcc tgctggtcgg ccagcggaaa
     3241 cacacccaca atgcgctcca cggttttcgc agcgtcaatc gtgtgcaacg tcgaaagtac
     3301 caggtggccg gtttcagatg cctcgagcgc gatttcgatg gtctcgcggt cgcgcattt
//
LOCUS       DASTPM010000124         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_205939, whole genome shotgun sequence.
ACCESSION   DASTPM010000124 DASTPM010000000
VERSION     DASTPM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205939"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1633
                     /locus_tag="VFR42_02155"
     CDS             98..1633
                     /locus_tag="VFR42_02155"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU0367983.1"
                     /translation="MQSFRRTASSLSLIALFAFSTDLALRTSAQANSQSSDTILIFNA
                     QLADGTGAPLREGALRIRGDRIVSIGKLSPAPGERVFDAHGLVLAPGFIDIHNHSLEG
                     LDSDPLAETQIAQGITTAVQGPDGESPWPIADWIAARRKNPAALNVAVFAGHATIREQ
                     VMGKDFKRVATQPEIEKMAQLTWQAMNEGAIGLSSGLEYEVGSYSNTAELVAMARAAA
                     EHGGFYSTHIRDEADKAFEALLEEIEIADQAHIPIDHSHIKLGTVGVWGKAYEYIRVI
                     SEARDRGVDFLADCYPYEAWHSNIKVIVPDKQYENPKSVEKALADMGGADHLTITAFK
                     PNPSYEHRSLAQLAKSNKLSPVEMYIRIIREGDAANTEAGVIGHSMIENDIKAFYQQP
                     WVMVASDGGIGIEHPRGAGTFPRVLGRFVREKHWLSLPDAIRKMTSLPAHRLNWPDRG
                     VLKEGAVADLVLFDPNTVLDRSSFEKPQELPIGIENVFVNGQMVWESGKPTGARPGTV
                     ITR"
     gene            1834..2952
                     /locus_tag="VFR42_02160"
     CDS             1834..2952
                     /locus_tag="VFR42_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEU0367984.1"
                     /translation="MNSKFRPWSLIVSLVLCSCSLLWVGGANAQSSQEYRRVNLVSDI
                     PGVALRSDPNLVNCWGIAFSPTSPVWIANNGTGTSTLYQGDGTPAPTPAAPLIVNIPS
                     PTAGATAAPTGLVFNAAGGFVVSSGAKSGPSIFIFSTEDGTIVGWSPAVDLHNAVIAI
                     PNSGSAVYKGLATGTSGGKPFIYATNFHDGVVEMYDSSFHLTKTFTDPGVPLGYAPFG
                     IQNINGDLYVTFALQNDEKHDDVAGLGHGFIDVFSTDGTWKSRFASNGVLNSPWGLAL
                     APPSFGKLAGRLLVGNFGDGRINAFDFPSGVPDGALRDTLGQPMTINGLWGLKFANGG
                     PSGRKNHLLFTAGIGDESHGLFGLIRPADAEDKHDDTE"
     gene            complement(3043..4302)
                     /locus_tag="VFR42_02165"
     CDS             complement(3043..4302)
                     /locus_tag="VFR42_02165"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HEU0367985.1"
                     /translation="MVKRILAIIAIFVCTCVAWMILGTSIFLRTDSAGTLLSGRVASA
                     WGSAQEQHPPTVSYLRPEQKLVEVEEDGKKVKKNETVQVTIPVALDASQIRADFHIDY
                     RQKGLLWFSAYRVAFNGEYKMRNPTQREQNFTITLPLPAQQAVYDTVEISLDGQKLPL
                     DFAGSSVRAHGSVAAGATGVVEAAYRSQGLDNWSYRFSEDNQVGRAKDFHLLLTTDFS
                     GFDFPENSLSPTEKSSKGKGWELKWDYQNLVSGFNIALKMPQKLQPGPLAGRISYFAP
                     VSLFFFFFLVFILSTLRGIEVHPMNYFFLACAFFAFHLLLAYLCDHISIHAAFLVSST
                     VSILLVVSYLRLVVNARFAVVDAGLSQLIYLVLFSYAFFFEGYTGLAITIGAILTLFV
                     VMQMTGRIRWEERLMKRIAEPPPLPAR"
     gene            4458..>4710
                     /locus_tag="VFR42_02170"
     CDS             4458..>4710
                     /locus_tag="VFR42_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367986.1"
                     /translation="MNRVVCSLFTLAFVPALSLGQAQTAAPSTSPIADALRQSLARNS
                     KNMAAAVEAMPPEKFSFKPTASQNSYGHLSVHIAESNFRF"
BASE COUNT         1006 a         1585 c         1121 g          998 t
ORIGIN      
        1 gcacccgctc cgagcaccag tagcacggac cctggtcgcg ttagcaagat cgcggctgca
       61 tctgctccgg cgcgttgcca acttcttgag gaacagaatg caatcattcc gacgaaccgc
      121 atcttcactt tctctaatcg cgctcttcgc cttttccacc gacctggctc ttcgcacttc
      181 ggcgcaagcg aattctcaat cgtccgacac gatcctcata ttcaacgccc agctcgccga
      241 cggcaccggc gctccactcc gcgaaggcgc tctccgcatc cgtggcgacc gcatcgtctc
      301 gatcggcaaa ctctcgccgg ctcccggcga acgtgtcttc gacgcccacg gcctggttct
      361 cgctcccggc ttcattgaca tccacaatca ctcactcgaa ggcctcgatt ccgatccttt
      421 ggccgagacg caaatcgccc aaggcattac cacagccgtg caaggccccg acggagaatc
      481 tccctggccc atagccgatt ggatcgccgc ccgccgcaaa aaccccgccg cactcaacgt
      541 cgctgtcttc gccggccacg cgaccatccg tgaacaagtc atgggcaagg acttcaagcg
      601 cgtcgccact cagcccgaaa ttgaaaaaat ggcgcagctc acttggcagg caatgaacga
      661 aggcgccatc ggcctctcca gcggccttga atacgaagtc ggcagctaca gcaacactgc
      721 cgaactcgtc gctatggccc gcgccgccgc cgaacatggc ggcttctaca gcacacatat
      781 ccgcgacgaa gccgacaaag cgttcgaagc attactcgaa gaaattgaaa tcgccgacca
      841 ggcccacata cccatcgacc attcccacat caagctcggc acggtcggcg tttggggcaa
      901 ggcctacgaa tacattcgcg tcatcagtga ggcgcgcgac cgcggcgtgg attttctggc
      961 agattgctat ccttatgaag cctggcactc caatatcaaa gtcattgttc ccgacaagca
     1021 gtatgaaaat cccaagagtg ttgaaaaagc gctagctgat atgggcggcg cggatcatct
     1081 gacaatcact gctttcaagc ccaatccttc ctatgaacac cgctctctcg cccaactcgc
     1141 gaagtccaac aaactttctc ccgtcgaaat gtacattcgc attattcgcg aaggcgacgc
     1201 cgccaacacc gaagctggcg tcatcggaca ttccatgatc gaaaacgata tcaaggcttt
     1261 ctatcaacag ccgtgggtca tggtcgcgag tgatggcggt attggtattg aacatccgcg
     1321 cggcgccggc acttttcctc gtgtccttgg ccgtttcgtt cgcgaaaaac actggctgtc
     1381 gcttcccgac gctatccgca aaatgacttc tcttccggcg catcgcctca actggcctga
     1441 tcgaggagtc ctcaaggaag gcgccgtcgc cgatctcgtc ctcttcgatc ccaacacggt
     1501 cctcgatcgc tcctcgttcg aaaaacccca agaacttccg atcggcatcg aaaatgtttt
     1561 cgtcaacggc cagatggtct gggagtccgg caagcccact ggcgcccgcc ccggcacggt
     1621 cattactcgc tgaaaaacgc cgcgaataca gccccagtca ggcgttttcc tgtacggctt
     1681 gtccctttcc cgtcaaacgc tggttaaaat ttcgtcaggc ccgccattac tttgctactc
     1741 cgtgctactc ctttcttgcg catcccgccc cttttcggat ctcccttttg gatccccttg
     1801 gtcccaaaga tcgcctggaa ggagaatcgc tctatgaaca gcaagtttcg gccctggtct
     1861 ctcatcgtgt ctctggtcct ttgctcgtgc tcactactct gggtcggcgg agcaaatgct
     1921 caaagttccc aggagtaccg ccgggtaaac ctcgtttccg acatccctgg cgtcgcactc
     1981 cgctccgacc ccaacctggt caactgctgg ggcatcgcct tctcgccaac cagcccggtg
     2041 tggattgcca acaacggcac cggcacctct actctctatc agggcgacgg cactcccgct
     2101 cccacaccgg cagcacctct gatcgtgaat attcccagcc cgacggcggg cgcaaccgcc
     2161 gcacccacgg gcttggtctt caatgcagct ggcggcttcg tggtttcctc cggcgcgaag
     2221 tccggccctt ccatcttcat cttctctacc gaagacggca caatcgtcgg ctggagcccc
     2281 gcagttgacc tgcacaacgc ggtcatcgcc atacctaact ccggctccgc cgtgtacaaa
     2341 gggctcgcca caggcaccag cggcggcaaa cccttcatct acgccaccaa tttccatgac
     2401 ggcgtcgttg aaatgtacga ttcctccttc cacctcacaa aaacgtttac cgatcctggc
     2461 gttccattgg gctacgcgcc gtttggcatt caaaacatca acggcgatct ttacgtcacc
     2521 tttgctcttc agaacgacga aaagcatgac gacgtcgccg gcctcggaca cggcttcatt
     2581 gatgttttct ccaccgacgg aacctggaag tcccgtttcg cctcaaacgg cgtcttaaat
     2641 tcgccgtggg gactcgcgct cgctccgccc agcttcggta aactcgctgg ccgcctgctg
     2701 gtgggcaatt tcggtgacgg ccgcatcaac gctttcgatt ttccgagcgg cgttcccgat
     2761 ggcgcgctga gggacactct cggccagccg atgaccatca acggcttgtg gggattgaag
     2821 ttcgcgaacg gcgggccctc aggcagaaaa aatcatctgc tcttcaccgc tggcatcgga
     2881 gacgagagcc acggcctttt cggcttgatt cgccctgccg acgccgaaga taaacacgac
     2941 gatactgaat agccatcgcg cttcctacgc tgctctgccc cgaagctgcg ccttcgccac
     3001 ttcctcccgg cgggggcgca gctcttctcc cggctgcgac gctcagcgcg ctggcagagg
     3061 cggcggttcg gcgatccttt tcataagccg ttcctcccag cggattcgcc cggtcatctg
     3121 catcaccacg aacagtgtca agattgctcc gatcgtgatt gctagcccgg tgtaaccctc
     3181 aaaaaagaac gcatacgaaa acagcaccag ataaatcagc tgcgacagcc cggcatctac
     3241 cacagcaaac cgggcattaa ccacaagtct caggtagctc accaccagta aaatggatac
     3301 cgtcgaggac accaggaacg cagcatggat cgaaatgtgg tcgcacaaat acgccaacaa
     3361 caaatgaaac gcaaaaaaag cgcacgccag aaagaaataa ttcattgggt gtacttcaat
     3421 tccccgcagc gtcgacaaga taaagaccag gaagaagaaa aagaacagcg acaccggcgc
     3481 gaaatacgaa atccgccccg ccaatggccc gggttgcagt ttctgcggca tcttcaacgc
     3541 aatgttgaac cccgaaacca ggttctgata gtcccatttc agctcccagc ctttgccctt
     3601 gctgctcttt tccgtaggtg acagggaatt ctccggaaaa tcgaaccccg aaaaatctgt
     3661 cgtcagcagc aagtgaaaat ccttggcccg ccccacctgg ttgtcctccg aaaaccggta
     3721 gctccagttg tcgagtccct gcgaacgata cgctgcctcg accactcccg tcgcgcccgc
     3781 cgccacgctt ccgtgggccc tgactgagga gcccgcaaaa tcgagcggca gcttctgtcc
     3841 atccagcgaa atctccacgg tgtcgtacac cgcctgctgc gccggcagcg gcaaagtaat
     3901 cgtaaaattc tgctcccgtt gtgtcgggtt gcgcatcttg tactcgccat tgaatgcaac
     3961 gcggtacgcg ctgaaccaca gcaatccttt ctgccggtaa tcgatgtgaa agtccgcgcg
     4021 aatctggctg gcgtccagcg ccactggtat cgtcacctgc acggtttcat tcttcttgac
     4081 cttcttcccg tcctcttcca cttcgaccaa tttctgctcc ggccgcaaat aacttaccgt
     4141 tggaggatgc tgctcttggg ctgatcccca tgccgacgct acccgcccgg aaagcagcgt
     4201 gcccgcgcta tccgtacgca agaagatcga agtgcccaag atcatccaag ccacacacgt
     4261 acacacaaag atggcgataa tcgccaaaat gcgtttcacc attggttttt ccccggtagc
     4321 tctctccgcc gcggtcttac cttctcacat ccactacgcc atgtcaagta ctttatgttg
     4381 caaagctgaa aaccctatct gcttgctgct ccgccccgac gggcgtaatc tgtctcgcgc
     4441 cactttctgg aggcttcatg aaccgcgttg tctgctcgct tttcacgctg gctttcgtgc
     4501 cggctctctc gctcggtcag gcgcaaaccg ccgcgccctc aaccagtccg atcgccgacg
     4561 cgctccgcca atctctcgct cgcaattcca agaatatggc cgccgctgtt gaagccatgc
     4621 ctcccgaaaa gttctctttc aaacctaccg cttcgcaaaa ttcctacgga cacctctcgg
     4681 tccacattgc tgagtccaat tttcgttttt
//
LOCUS       DASTPM010000125         4235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_32817, whole genome shotgun sequence.
ACCESSION   DASTPM010000125 DASTPM010000000
VERSION     DASTPM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32817"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VFR42_02175"
     CDS             complement(<1..854)
                     /locus_tag="VFR42_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367987.1"
                     /translation="MSDVAKTCSQPGRKRRGKRQDGYAIMLVLSMATLMLIATMAVAP
                     NILNQGRREREAEMVWRGKQYVRAIKMYYRKTGKFPTSLEDLTKPKIGNIRFMRQTYK
                     DPMNGKDGEWRLIYVGPAGQLIGSLKPQRALQLTGLGGFQNMTAPNIGGAGLPGGQQT
                     FGQGLGQAFGQASSQIMGNSTGMTTGGMQGPQNQLAGVSADTTALGTTNSTDPSDTDT
                     AENAALLNSEPRTIMGGNIIGVGSKINQRSLMVYDKAKNYRQFEFIWDPSKDVMVVGS
                     APPAGATPA"
     gene            complement(851..1387)
                     /locus_tag="VFR42_02180"
     CDS             complement(851..1387)
                     /locus_tag="VFR42_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367988.1"
                     /translation="MKQQKQIIALVVLMIAATAIWFYNSRQNPIAAGASTIAWNYSPM
                     NVDNLKLRTAQRDASRISEYKGTGRDPFNATPPPPPPAPIPKPGDKDYVPPPPPPPPR
                     AELPANMKYYGYGTVPNGTARRAYLTDGDDVYIVAEGDTLLGRFRIVKIGNATLEFED
                     ISSHVQGQKNMEDAGPVS"
     gene            complement(1384..1938)
                     /gene="pilO"
                     /locus_tag="VFR42_02185"
     CDS             complement(1384..1938)
                     /gene="pilO"
                     /locus_tag="VFR42_02185"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU0367989.1"
                     /translation="MDRTLTVKRRIIFAGLVILLAGDLALGAYSWHTANALRRPMAEL
                     VADSRKLQLLRIDVDNADRIRHDLPATVAECDRFETGLLAASTGNSSVTAELDELAKK
                     SGLQIQALTFRHKDLAGRNLTQIDMDLAVSGTYSNIVKFMNSLQRSKSSYAVDALVLQ
                     PASQNTPNGLHIGMHMKTYFRTGA"
     gene            complement(1938..2507)
                     /locus_tag="VFR42_02190"
     CDS             complement(1938..2507)
                     /locus_tag="VFR42_02190"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU0367990.1"
                     /translation="MNVRLNLATKPLESQRKFYVSAGLVAFLGSVLFLVLGWHVYSEI
                     RAKEDLRRKEQDNDRVAAQLTARRRELDDFFGRQENAKLKDRVAFLNGFLNESSFDWT
                     RMFMDLEKTLPVGVHVLSIQPKFEKGHMSVHLTVGAVSDEAKVKFLRAMENSPAFNNV
                     TLINVRRPSGVQGETDQAVMELNAIYSRT"
     gene            complement(2523..3500)
                     /locus_tag="VFR42_02195"
     CDS             complement(2523..3500)
                     /locus_tag="VFR42_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367991.1"
                     /translation="MTEPAAYRHGIYKSDLVRKVALWLEAQPHPPVAIEIAPDRISGA
                     RFSRTGSLDGFAVEALPAGAIVPSAVETNLVNLAAVRTAMEGIRERLRVREEDATLLL
                     PDPVVRVFVQHFEQFPRSAKEAIPMLRWKLKKSVPFEAEETLISYMRQAPREGGVDVV
                     TALGRLRIVREYEALAEQAGFHPGVVLSSSLAAVSLLEDRRPALLARISGASLTTAIV
                     REGVLCGYRCTDLPAQVAQLTPQMLLEEIYPVAAYYQDTWQEGIQSVRVAGLAGRLPE
                     FVPPLENEFHCEVHSLLHTAISEGRVSNDARPLAERELEGLVGWMMQRN"
     gene            complement(3493..>4235)
                     /locus_tag="VFR42_02200"
     CDS             complement(3493..>4235)
                     /locus_tag="VFR42_02200"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU0367992.1"
                     /translation="LVTGPTGSGKTTTLYAAINEIKTDEDKIITIEDPVEYQLRGITQ
                     IPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAIQSALTGHLVFTTVHANN
                     VTDVIGRFINMGVEPYNFVSALNCIMAQRLVRIICSNCKRPKKLSSEEVKESGLQPED
                     LEHARISEGAGCLECSGTGYHGRTAICELLDLSDRIREMIINRRPTSEIKRVAREEGM
                     ITLRESGLAKVRMGATTLREINKVTFVD"
BASE COUNT          848 a         1239 c         1195 g          953 t
ORIGIN      
        1 gctggagtgg ctccggccgg cggtgcgcta cccacgacca taacgtcctt ggacggatcc
       61 cagataaatt caaactgccg atagtttttc gccttgtcgt acaccatcag tgagcgctgg
      121 ttgatcttgc tgccaacgcc gatgatgttg ccgcccatga tggtgcgcgg ttcggagttc
      181 aagagggccg cgttttcggc tgtgtcggta tccgagggat cggtggagtt ggtcgttccc
      241 aatgccgtag tgtcagccga aactcctgcg agctgatttt gtggtccctg cataccgccc
      301 gtcgtcattc cggtgctatt gcccatgatt tggctgcttg cctgaccaaa tgcctggcca
      361 agaccctgac caaaggtctg ctgaccacca ggcaatccgg ctccgccaat attgggcgcc
      421 gtcatgtttt gaaagccgcc caaaccagta agctgcaacg cacgctgggg ctttaggctc
      481 ccgatcagtt ggccggccgg accaacatag atcaggcgcc actcgccgtc tttgccgttc
      541 atcggatcct tgtaggtctg ccgcatgaaa cggatattgc cgatttttgg cttggtaagg
      601 tcttccagcg aggttgggaa cttaccggtc ttgcggtaat acatcttgat ggcccggacg
      661 tactgcttgc cgcgccagac catctctgct tcgcgctccc ggcgaccctg attcaggata
      721 ttgggcgcga cggccatggt ggcgatcagc atcagcgtgg ccatggacaa aacgagcatg
      781 atggcgtaac cgtcttgccg cttaccgcga cgcttgcggc caggctggct gcaagttttg
      841 gcgacgtcgc tcacgatacc ggccccgcgt cttccatgtt cttctgccct tgaacatgtg
      901 aagaaatatc ttcaaattcg agcgtagcgt ttccgatttt aacgatgcgg aaacgtccca
      961 agagcgtgtc accctcggcc acaatgtaga cgtcgtcgcc gtcggtcaaa taagcgcgcc
     1021 gcgcggtccc atttggaacg gttccgtagc cgtaatattt catattggcc ggcagttccg
     1081 ctctgggagg gggcggcgga ggcggtggaa cataatcctt gtcacccggc ttaggtatgg
     1141 gagcaggcgg cggaggcggt ggcgtggcat tgaacgggtc ccgacccgtg cccttgtatt
     1201 ccgaaatgcg gctcgcgtcg cgttgggccg tgcgcagctt gaggttgtcc acattcatcg
     1261 gcgaatagtt ccacgcgatg gtgctggcgc ccgccgcgat aggattctgg cggctgttat
     1321 aaaaccagat cgcggttgcc gcgatcatta aaacgaccaa cgcgataatt tgcttctgct
     1381 gcttcatgca ccggtcctga aatatgtttt catgtgcatg ccgatgtgca gaccattcgg
     1441 cgtattctgc gacgccggct gcagcaccag ggcgtcgact gcgtacgaac tcttcgaacg
     1501 ctgcaggctg ttcatgaact tgacgatgtt ggagtaagtc ccgctcaccg ccagatccat
     1561 gtctatttga gtcaagttcc ggccggcaag gtctttgtgg cgaaaggtca gtgcctgaat
     1621 ttgcagtccc gattttttcg caagttcatc gagctcggct gtaaccgaag aattgcccgt
     1681 gctggccgca agcaaccctg tctcgaagcg gtcgcattcc gccaccgtag caggcaaatc
     1741 gtggcgaatg cggtcggcgt tgtctacatc aatgcgcaag agctgcaatt tgcgcgaatc
     1801 ggcaaccaac tccgccatgg gcctacgcag cgcgttcgcc gtgtgccagc tgtaggcgcc
     1861 cagcgcgaga tcgccggcca gcaggatgac aagtcccgca aagattattc ttcgtttgac
     1921 ggtcagcgta cggtccatca agtcctcgag tagattgcgt tcagttccat cactgcctga
     1981 tcggtttcac cctgcacgcc ggagggacgg cgtacgttga tcagcgtaac gttgttgaac
     2041 gccggcgaat tctccatggc gcgcagaaac ttgaccttgg cctcatcgga aactgctccg
     2101 accgtgagat gcacggacat atgccccttt tcgaactttg gctgaatgct gagaacgtgg
     2161 acgcctaccg gcagggtctt ctccaggtcc ataaacatac gggtccagtc gaaactgctc
     2221 tcgttaagga atccgttgag aaatgccacg cggtctttga gtttggcgtt ttcctggcgc
     2281 ccgaaaaagt cgtccagttc cctgcggcgc gctgtcagct gggcggccac ccggtcgttg
     2341 tcttgctctt tacggcgcaa gtcttccttg gctcgaattt ccgaatacac gtgccaaccg
     2401 agaaccagaa aaagcacgct gcccaaaaag gcgacgagcc cggcagatac gtagaacttc
     2461 ctctgcgatt cgagcggctt ggtggcgaga tttaagcgga cattcatcga aaaatcctgt
     2521 gctcagttcc tttgcatcat ccagccgacc agtccttcca gttccctctc cgccaacggc
     2581 cgcgcatcgt tggaaacgcg tccctccgaa atggccgtgt gcagcagcga atgcacttcg
     2641 cagtgaaatt cattttcgag aggcggcaca aattccggta agcgcccagc aagtccagcg
     2701 acacgcacgg actgaatgcc ttcttgccag gtgtcctggt aataggccgc taccggatag
     2761 atctcttcca aaagcatctg cggggtgagc tgcgcgacct gcgcaggcaa atcggtgcaa
     2821 cgatagccac acaaaacgcc ttcccgcaca atcgcagtag tcagagacgc gccagaaatg
     2881 cgagcgagca aggccggcct gcggtcttcc agcaaggaaa ccgcggcaag cgacgaactc
     2941 agcactacgc ccggatgaaa tcccgcttgt tcggccagtg cttcgtactc gcgcacaatc
     3001 cgcaagcgtc ccagtgcagt cacaacatcc acaccgcctt ctcggggagc ctggcgcatg
     3061 taggagatca acgtttcttc cgcttcgaac ggaacacttt ttttcaattt ccagcgaagc
     3121 atcgggatcg cttctttagc gctgcgcgga aactgctcga aatgctggac gaatacgcga
     3181 acgaccggat cgggtaacag cagcgtcgcg tcctcttcgc gcacgcggag gcgctcgcgg
     3241 ataccttcca tcgccgtgcg cacggccgca aggttgacca gatttgtctc caccgcagag
     3301 ggaacaatgg cgcctgccgg cagagcttcc accgcgaagc cgtccagaga tccagtccgc
     3361 gaaaatcgcg cgccggaaat ccggtctggc gcgatttcga tcgccaccgg aggatgcggt
     3421 tgcgcttcca gccaaagcgc gactttgcgc accaggtcgc tcttgtaaat gccgtgtcgg
     3481 tatgcggcag gctcagtcaa cgaaagtcac cttgttaatt tcccgcagcg tggtagcccc
     3541 catacgaacc ttggctaggc cgctttctct caatgtgatc ataccttctt cgcgggcaac
     3601 tcgtttgatc tctgaggtcg ggcggcgatt aatgatcatc tcccggatgc ggtcggaaag
     3661 gtcgagaagt tcgcaaatcg cggtccggcc gtgatatccg gtgccagaac attccaggca
     3721 gcccgccccc tcgctaatcc tggcatgttc caggtcttcc ggctgaaggc cggactcctt
     3781 cacctcttcg gacgacagtt tcttggggcg cttgcagttg gaacagatga tacgcaccag
     3841 acgctgcgcc atgatgcagt tcaatgccga aacaaagttg taaggctcca cgcccatatt
     3901 gatgaagcgg ccgatgacgt ccgtaacgtt gttggcgtga acggtagtga aaaccaggtg
     3961 tcccgtgagc gcggactgaa tcgcgatctg ggcggtttcc tgatcgcgaa tttcgccgac
     4021 catgattttg tccgggtcgt gacgcaaaat cgaccgtaac ccacgggcga acgtcagccc
     4081 cttcttctcg ttcaccggga tttgtgtgat gccgcgcaat tgatattcga ccgggtcttc
     4141 gatggtgatg attttgtctt cgtcggtttt gatttcgtta atcgccgcgt acagcgtggt
     4201 ggtctttccg gaacctgtcg gtcctgtgac cagca
//
LOCUS       DASTPM010000126         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1301527, whole genome shotgun sequence.
ACCESSION   DASTPM010000126 DASTPM010000000
VERSION     DASTPM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301527"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFR42_02205"
     CDS             complement(<1..210)
                     /locus_tag="VFR42_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367993.1"
                     /translation="MQIKILGSAAGGGFPQWNCACPNCSGIRSGQLQATPRTQTQLAF
                     SPDGSISFLLGASPDLRTQILATPQL"
     gene            1031..1471
                     /locus_tag="VFR42_02210"
     CDS             1031..1471
                     /locus_tag="VFR42_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0367994.1"
                     /translation="MSLRGNCRATVDEKGRLKIPAVFLEGLREYGNQFYITSVTGEAA
                     LIYPLKIWAGIEERLAKVSSQNQAKSKFLKRTSYYGQVVEMDAQGRVLVPAVLRESAQ
                     AKGDVDVFGSLDHLEVMNHTRVMDQMKNEPFTADDYKALEDLGI"
     gene            1528..2421
                     /gene="rsmH"
                     /locus_tag="VFR42_02215"
     CDS             1528..2421
                     /gene="rsmH"
                     /locus_tag="VFR42_02215"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU0367995.1"
                     /translation="MNAPHTPVMLQEVLEWLRIKADGIYLDATAGAAGHSEAIAERLS
                     LQGRLISLDRDAQALEIARERLKRFGSRVSCVLSPFSRIAEGAHELGIPKVDGVLADL
                     GVSSMQLDQAERGFSFREAGPLNMRMGVSAEGEASELTADEIVNRWSEKELADLLYRE
                     ADEHDSRRIARAVVRARPIRDTAHLATVVAGSRKQWGRQRLHPATKTFLALRIAVNRE
                     MEELGQFLSRVPATMNSGGRCVILTYHSREDRIVKAAFREAERQGELKVLTKHVVRPS
                     EDEERANPRSRSAKLRCAEKV"
     gene            2426..2839
                     /locus_tag="VFR42_02220"
     CDS             2426..2839
                     /locus_tag="VFR42_02220"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU0367996.1"
                     /translation="MSKKKAALLMEFRTVKRIDNSRLVRHVEPAKQKNLFKTAGLGGV
                     VALFFLFYIFQHFKCIDLSFQLEDLKVQQAQATQLNSELKLEIANLRDPMRIDVIARR
                     QLGLTEPLPTQMREYEAPSGMQVAAARFVRRGQQR"
     gene            2913..>4658
                     /locus_tag="VFR42_02225"
     CDS             2913..>4658
                     /locus_tag="VFR42_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU0367997.1"
                     /translation="MQEEKVHVRLLIVAGVALLWMTAVFGRLGYLQLIRHSDYLARAQ
                     RQQQRTIEITPKRGAIYDRNLHPLAMSVPVESAFAVPSEIGDNKIMAARLLSNVLGVP
                     RDVLQARFESGGSFVWVLRKLPPDKVEAVRALNLKGVYLQAENQRYYPKRELAAHVLG
                     FVDLDEKGLGGIEYGLDSLIRGKSEKIVVMADARQRWFDGGSAQKDQGANVILTIDEK
                     IQYIAERELAAAIAKTHAMAGTVIVQNPNNGAILALANWPKFNPNSPTDAKADARMDR
                     AVSAIYEPGSTFKLITLAAALDQNLTRPDEVFNCENGAVYIAGHRIRDHKPFGMLTVS
                     DILAKSSDVGAIKIALRMGAPKFYEYIRVFGFGQQTGVELPGESKGILRKLDNWSAIS
                     IGSVSMGQEVGVTPMQLVTAVSAIANGGTLYKPHVVAEVKRGSESLPMAGVLAPGEPK
                     EAIRPETAATLRRLMEGVVLKGGTGTRARLDGWTAAGKTGSAQKIDPATGRYSPTQLI
                     ASFSGFAPINDPAVTILVSLDSPVGEHEGGQVAAPVFKRIAEQVLPYLDVPRDVPLSP
                     KLIQAAYKRNEMEEES"
BASE COUNT         1063 a         1066 c         1641 g          888 t
ORIGIN      
        1 gagttgcggc gtggccagga tttgggtgcg gaggtcggga gaggcgccga ggagaaacga
       61 gatagaaccg tcaggggaga aggcgagttg agtttgagtt cgtggcgtgg cttggagttg
      121 accagagcgg atgccggagc agttggggca agcgcagttc cattgcggga agccgccgcc
      181 ggctgcggag ccgaggatct taatttgcat ggtcgagtgt catgccggga atggagtcgc
      241 gccgatgcgg cgatcgcatg tgctcactct agcaccgaac gtcctcggtg atgctgacgc
      301 cgaggccctt cgcgtcgctg ccgcgccgct taggatacaa acttgaggcc gaaggcttga
      361 gcaaaagaaa aggccggacg ttgagtccgg cgaagaaatt ttggagtaga aaaagacctg
      421 gggcccgaag aacgccgtta gcgcttagat ttcagcgttg gcgtaggagc tgatttcgca
      481 gttgaggtcg atttcttcgt gctgcggtgt ggtccattcc atgggttaag actccttggt
      541 attggtcaaa caaataaagc ataacacggg cggaaaggaa cgcgaaggag aatcggttga
      601 gtgacgagag tggcgctcgc cagaacgccg ctttcccaag cgacgtgcta gcggtcgcgg
      661 gcgcgcaaaa gcggagcgag aatctcagaa gaagcgccgc tagcggtggt gagttgcaac
      721 gagagggtac acaaccggtg gcggtaagat tatatgcacc atcgaggaac agttttccaa
      781 aaaatatttc gagaaattga cgatttcgct tgacaagaaa tggaacgagt ttaatatgtg
      841 gcgaaaagtg gtacgaagtg gttggtagtg gatggaagta gggcagggta gggctttgag
      901 ggcgcggtcg taagaacacg aaaatttggg cggatttagg gcgatttgac gatctggggc
      961 aacagattgg caagtacata ccctgagtat cggttgtggg cgtcgccgag ctaccggcga
     1021 tcaggtgcaa atgagtcttc ggggaaactg tcgggcgacg gtggacgaga agggtcgtct
     1081 caagataccc gccgtgtttc tcgaggggct gcgggagtac gggaatcagt tttacatcac
     1141 cagcgtaacc ggtgaagcgg cgctgattta cccgctgaag atttgggcgg ggattgaaga
     1201 gaggctggcg aaggtgtcca gccaaaatca ggccaagagc aagtttttga agcggaccag
     1261 ctactacggg caagtggtag agatggacgc gcagggcagg gtgctggttc cggcggtgtt
     1321 gcgggaatcg gcgcaggcta agggcgacgt ggacgtgttc gggagtttgg accatctgga
     1381 agtgatgaat catacgagag tgatggacca gatgaagaac gaaccgttta cggcggacga
     1441 ctacaaggcg ttggaagatt tgggcattta gggcaagcaa gtcagcggtt atcgggcggg
     1501 caattgggct acggcggagg tgagcgagtg aatgcgccac atacgccggt gatgttgcaa
     1561 gaggtgctgg aatggctgcg aataaaggcc gacggaatct acctagacgc cacagcggga
     1621 gcagcggggc attcagaagc catcgccgaa agactctctt tgcagggccg gttgatcagc
     1681 ctggaccggg acgcacaggc gctggaaatc gcgcgggagc gtttgaagag gttcggatcg
     1741 agagtgagct gcgttttgtc tccgttctca aggattgcgg agggcgcaca cgagttgggg
     1801 attccgaagg tggacggggt cttggccgac ttgggggttt cgagcatgca gcttgaccag
     1861 gcggaacggg gattttcatt ccgcgaagct ggtccgctga acatgcgaat gggagtaagc
     1921 gctgaaggcg aagcgagcga gttgacggca gacgagattg tgaatcggtg gtcagagaaa
     1981 gagctggccg atttgctcta ccgcgaggcg gatgagcacg attcgagaag gatcgccagg
     2041 gcagtggtaa gagcgcggcc gatcagagac acggcgcacc tggcaacggt tgtggcaggg
     2101 tcccgaaagc aatggggaag gcagagactg catcccgcga caaaaacgtt tctggcgctg
     2161 cggatagcgg tgaatcgaga gatggaggaa ctcgggcagt ttctttctcg ggtccccgcc
     2221 acaatgaatt caggagggcg gtgcgtgatt ttgacgtacc actcgcgtga agaccggatc
     2281 gtgaaagcag cgttccggga agcagagcgg caaggcgagc tgaaagtttt gacgaagcac
     2341 gtggtgaggc ccagcgagga tgaagagcgg gcgaacccgc ggtcgcgaag cgcgaaactg
     2401 cggtgcgcgg aaaaggtctg acgttatgag caagaaaaaa gcagcactgc tgatggagtt
     2461 ccggacggta aagcgcatcg ataattcacg gctggtgcgg catgtggagc cagcgaagca
     2521 aaagaatctt ttcaagaccg caggactcgg cggcgtggtg gccctgtttt ttcttttcta
     2581 catttttcag catttcaagt gcatcgattt gagctttcag ctggaagatt tgaaggtgca
     2641 gcaggcgcag gcgacgcagc tgaattcgga gttgaagctg gagatcgcga atctgcgcga
     2701 tcccatgcgc attgacgtga ttgcgcgaag gcagttgggg ctgacggagc cgctgccgac
     2761 gcaaatgcgc gagtatgaag cgccgagcgg gatgcaggtc gcggcggcgc gatttgtgcg
     2821 ccggggccag cagcggtaga cccgcagtat gaaacgagct tcgcaagagc agtcgacctt
     2881 gatcacggag agaatcccga acactgcggg ccatgcaaga agaaaaagtc cacgtccgtt
     2941 tgctgatcgt cgccggcgtg gctttgttgt ggatgacggc ggttttcggt cgtctcggtt
     3001 atctccagtt aatccggcat agcgactacc tggcacgcgc gcagcggcag cagcagcgca
     3061 ccatcgaaat caccccgaag cgcggcgcaa tctacgaccg caacctgcat ccgctggcga
     3121 tgagcgtgcc ggtggagtcc gcgtttgcgg taccttccga aatcggcgac aacaaaatca
     3181 tggcggcgcg gctgctttcg aatgtgttgg gggttccgcg cgacgttttg caggcgcggt
     3241 tcgaatcggg cgggtccttt gtctgggtac tgaggaaact gccgccggac aaagtggaag
     3301 cggtacgcgc actgaattta aagggcgtgt acctgcaggc tgagaaccag cgctattacc
     3361 ccaagcgtga gctggccgcg cacgtcctgg gatttgtgga tctggatgaa aaaggtttgg
     3421 gcggaatcga gtacgggttg gacagcctga tccgcggcaa gagcgaaaag attgtagtga
     3481 tggcggatgc gcggcagaga tggttcgacg ggggctcggc gcaaaaagat caaggtgcga
     3541 acgtcatcct gacgatcgac gagaaaattc agtacatcgc ggaacgtgag ctggcggcgg
     3601 cgatcgcgaa gacgcacgcg atggccggta ccgtgattgt gcagaatccg aataatggag
     3661 cgattttggc gctggccaac tggccgaagt tcaatccgaa ttcgcccact gacgcgaagg
     3721 ccgacgcgcg aatggaccgc gccgtgagcg cgatttatga gccgggatcg accttcaagc
     3781 tgattacact ggcggcggcg cttgatcaaa acctgacgcg gccggacgag gtgttcaact
     3841 gcgaaaacgg cgcggtgtac atcgcgggtc acaggattcg cgaccacaag ccgtttggga
     3901 tgctgacggt gtcggacatt ctagcgaagt cgagcgacgt gggagcgatc aagattgccc
     3961 tgcgcatggg cgctccgaaa ttttatgaat atattcgcgt gtttggattt ggccagcaga
     4021 caggcgtgga attgcccggt gagagcaaag gcatcttgag gaaactggac aactggagcg
     4081 cgatttccat cggttccgtt tccatgggcc aggaagtggg cgttacgccc atgcagctgg
     4141 taacggcggt ttcggcgatc gccaacggcg ggacattgta taagccgcac gtggttgcgg
     4201 aagtgaagcg cggctcggaa tctctgccaa tggccggtgt gctagcgccg ggcgagccga
     4261 aggaagcgat tcgtccggag acggctgcga cgctgcgacg gctgatggaa ggggtggttt
     4321 taaaaggcgg tacggggacg agggctcggt tggacggctg gacggcagcg ggaaagacag
     4381 gctctgccca aaagatcgat ccggcgacgg ggcggtattc gccaacgcag ctgattgctt
     4441 cgttttcggg attcgcgccc atcaatgatc cggcggtgac gattttggtt tcgctcgatt
     4501 cgccggtcgg agagcacgag ggagggcagg ttgcggcgcc ggtgtttaaa aggatcgcag
     4561 agcaggtgct gccgtatctg gatgtaccgc gtgacgtgcc gctgagcccg aaactgatac
     4621 aggcggcgta caagcggaat gagatggaag aggaatca
//
LOCUS       DASTPM010000127         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_379194, whole genome shotgun sequence.
ACCESSION   DASTPM010000127 DASTPM010000000
VERSION     DASTPM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379194"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VFR42_02230"
     CDS             <1..328
                     /locus_tag="VFR42_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU0367998.1"
                     /translation="HGKDRYGKKLGQYYIHGLGHHIGLDVHDPGEYCKPLQPGMVVTM
                     EPGIYIPEENLGVRIEDDVLITDSGYEQLSQRLPRDPAEIEKIMADAARQRAGLAAKA
                     TPVLGD"
     gene            446..1018
                     /locus_tag="VFR42_02235"
     CDS             446..1018
                     /locus_tag="VFR42_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0367999.1"
                     /translation="MTNLSAKHPSSFSGARIAARVAELGTQITRAYNGRRMDVVITMD
                     RGFVFAADLVRQIAVPLVCHFVREQVSDVQQGKHARREVFFTGARPELKGRDVLLVDA
                     VLDSGVTQEFLLRRLADSQPRSLRLAVLLDKPESRRVALEPDYFGFQTASNQMWVGYG
                     LADASGAGRNARSLSAKRISASNKKGQRRR"
     gene            1070..3016
                     /gene="ftsH"
                     /locus_tag="VFR42_02240"
     CDS             1070..3016
                     /gene="ftsH"
                     /locus_tag="VFR42_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0368000.1"
                     /translation="MNPSSTAKTILFWLSIVLLGVMLWKLVSANGQAAREDEPSFSEF
                     MAHVDQGDVKEVTMYLSPNSYELQGEYAKASKKFHVTVFKEAAPDLTKQLRDKSVLIK
                     VKEVRSGDWLIILLNALPLLLLVGFCLFLMRQMQAGGNKALSFGKSRARLLSAQQKKV
                     TFKDVAGTDEAKEELQEIIEFLKDPQKFQKLGGRIPKGVLLVGPPGTGKTLLARAIAG
                     EANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLG
                     GGHDEREQTLNALLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRRVVVARPD
                     VKGREEILRVHTRKKPIADDVDLSIIARGTPGFSGADLESLANEAALWAARQNRKLVT
                     MADFEMAKDKVLMGVERKSMILSDEEKKNTAYHEAGHALVAAMTPGADPVHKVTIIPR
                     GMALGLTMQLPTDDKHSYTKDHLEGMLAVLMGGRSAEEIFLGHITTGAGNDIERATDI
                     ARNMVCEWGMSELGPLAFGKKEEAIFLGREIAQHRDFSEATAVDIDKEVKRIVSSAYD
                     NAKGILNTHRDVLERIAQALLEREVLDANEVRMLIEGTPLPEKPRTPPPSTPPLAPAA
                     DPKVVRPELRPAPGFTRGDSPAKA"
     gene            complement(3265..4029)
                     /locus_tag="VFR42_02245"
     CDS             complement(3265..4029)
                     /locus_tag="VFR42_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0368001.1"
                     /translation="MSAHMEKESSLNSLEMDREVIRCKTCGLVQYRTRTGNCRRCVRA
                     LPQRLEFLIPPAPPLEEQGVEVSTDKFANQQTVENIGQRIRQLRESRGMTQSQLQSKS
                     KVSRSYLSRIESGQMTPSLGTLEKISEALNVGLNRFFIPESDGEALLEDPFIQGLRPF
                     LQQLDWEQWQSILKRLQAISDHVGGAPVNLRPIGNPARLPQPAAVSGAGSMDPVATRV
                     NSVFRPAASSQALGHPQGYPLRRPGTSPIYSRPLAR"
     gene            complement(4152..>4388)
                     /locus_tag="VFR42_02250"
     CDS             complement(4152..>4388)
                     /locus_tag="VFR42_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368002.1"
                     /translation="TLRSAAVEMCNRTTIVAYDTRPAALRTQSGHEDCGGLKERTQGV
                     GSFWRERRKDGRKEAKIWRKPSASPTEKMGNLKK"
BASE COUNT          925 a         1370 c         1194 g          899 t
ORIGIN      
        1 gcatggcaag gaccggtacg gaaaaaaact cggccaatac tacatccacg ggctcggcca
       61 ccacatcggt ctcgatgtgc acgacccagg tgagtactgc aagcccttgc agcccggaat
      121 ggtggtcacc atggagcccg gcatttacat tcctgaagag aacctcggcg tgcgcatcga
      181 agacgacgtc ctcatcaccg actccggata cgaacagctc agccagcgcc tgccccgcga
      241 tcccgcggag atcgagaaga tcatggccga cgccgctcgc cagcgcgcag ggttggccgc
      301 caaggcaact cctgttctcg gtgattgact gccattgcct caggcgagac actatttaga
      361 gaggaaggta gctgcatcag ggtaacgttg tcccgcaacg ccgctagaac gggtttcaga
      421 gctgttttca gtctctggaa aaacaatgac gaacttgagc gcgaaacacc cttcttcttt
      481 ttccggagca cgcattgcgg cgcgagtggc ggagttaggc acgcagatta cccgtgccta
      541 caacggccgg cgtatggacg tggtaattac catggatcgc ggatttgtct ttgccgccga
      601 tctcgtccgg caaattgctg tgccgctggt ttgccacttt gttcgcgaac aggtaagcga
      661 cgtgcagcag ggcaaacatg cgcggcgtga agtttttttc actggcgcgc gcccggaatt
      721 gaaaggccgc gacgtcctgc tggtcgatgc agttctggac agcggcgtca cgcaggagtt
      781 tttgttgcgg cgtctggcag acagccagcc gcgttcgctg cgcttggccg ttttgctgga
      841 taagccagaa agccgtcgag tcgccttgga gcccgattac tttggcttcc aaaccgcatc
      901 taaccagatg tgggtcggtt acggtttggc tgatgcttcg ggcgcaggga gaaacgcccg
      961 tagcctgtcg gcaaagcgca tttcggcgtc taataagaag gggcagcgac gcaggtaggt
     1021 caagcgctcg aatttccgat ttttcggtac ccagttggag aggtaaaacg tgaaccccag
     1081 ttcgacagca aaaacgattt tattctggct ctcgatcgtg cttctaggcg tcatgctctg
     1141 gaagctcgtt tcggccaacg gtcaggcggc tcgcgaagac gagcccagct tcagcgagtt
     1201 catggcccac gtcgatcagg gcgacgtcaa agaggtcacc atgtacctct cgccaaacag
     1261 ctatgagctg caaggcgaat atgccaaggc cagcaagaaa ttccacgtca ccgttttcaa
     1321 ggaagccgct cccgatctca ccaaacaact ccgcgataaa tcggttctga ttaaagtaaa
     1381 agaagtccgc agcggtgatt ggctgatcat tcttttaaac gccctgccgc tccttcttct
     1441 cgtaggcttc tgcttgttcc tcatgcgcca gatgcaggcg ggcggcaaca aggctctaag
     1501 tttcggcaaa tcgcgagctc gcttgctttc cgctcagcag aaaaaagtta cctttaaaga
     1561 tgtcgccgga actgacgaag cgaaggaaga actccaggaa attatcgagt tcctcaaaga
     1621 cccgcagaaa ttccagaaac tcggcggccg cattcccaaa ggcgttctcc tggtcggacc
     1681 tccaggcacc ggcaagactt tgcttgcgcg cgccatcgcc ggtgaagcca atgttccctt
     1741 cttctccatc tccggttccg acttcgtcga aatgtttgtc ggcgtaggcg ccagccgcgt
     1801 gcgcgatctt ttcgagcaag gcaaaaagaa tgcgccctgc atcatcttca tcgacgagat
     1861 tgacgccgtt ggccgccatc gcggcgccgg actcggcggt ggtcacgatg agcgcgaaca
     1921 aactctaaac gccctgctcg ttgaaatgga cggctttgaa tcgaacgaag gcgtcattct
     1981 gatcgccgcc accaaccgtc ccgacgttct tgatcccgct ttactgcgcc ccggtcgttt
     2041 cgatcgtcgc gtcgttgtgg ctcggcccga cgtcaaggga cgcgaagaaa ttctccgcgt
     2101 gcacacgcgc aaaaagccca tcgccgacga tgtcgatctt tccatcatcg ctcgcggcac
     2161 tcccggattc tcgggcgccg atctcgagag cctcgccaac gaagccgctc tctgggccgc
     2221 acgtcagaac cgcaagctcg tcaccatggc cgactttgaa atggccaagg ataaagtcct
     2281 catgggcgtt gaacgcaagt ccatgattct gtccgatgag gaaaagaaaa acactgccta
     2341 ccatgaagcc ggccacgctc tcgtagccgc tatgactccc ggcgccgacc cggttcacaa
     2401 agtcaccatc atcccgcgcg gcatggctct cggcctcacc atgcagttgc cgaccgacga
     2461 caaacattcc tacaccaaag accatctcga aggcatgctc gccgttctca tgggtggccg
     2521 cagcgccgaa gaaatcttcc tcggccacat caccaccggc gccggcaatg acattgaacg
     2581 agccaccgac atcgcccgca acatggtctg cgaatggggc atgagcgaac tcggccctct
     2641 cgccttcggc aagaaagaag aagccatctt cctcggccgc gaaatcgccc agcaccgcga
     2701 cttcagcgaa gccactgccg tggacatcga caaggaagtg aagcgcatcg tcagctcggc
     2761 ctatgacaac gccaaaggca tcctgaatac gcatcgggac gtgctggagc gtatcgccca
     2821 ggctcttctc gaacgggaag tcctcgatgc caatgaagtc cgcatgctca tcgaaggcac
     2881 tccgctgccc gaaaaaccgc ggactccgcc gccgtctact ccgccgttgg cccctgcagc
     2941 cgatcccaag gtggtgcgcc ccgaactgcg ccccgctcct ggcttcaccc gcggggacag
     3001 ccccgctaag gcttaataag tctcaggact aagctcgaat accgccggca aatccatcca
     3061 gcggcactaa ctgaaacaga agaagggcgc ggcgcgctct tctttcttgg acccacaggc
     3121 ttccgcccgt gcctcggttt caactagctc aggccccctg ctctgtaccc ggatacacct
     3181 cgtccaaaaa atcagggcgt gccgaagcac gcccttaggg gacagatttc aattgtggtt
     3241 tcgcgcccaa caggcgcgaa acgactagcg agcgagcggc cgcgaataaa tcggcgacgt
     3301 gcccggccgg cgcagcggat atccttgcgg atgcccaagc gcctgactgg aagctgccgg
     3361 acggaacacc gagttcactc gcgtggcgac gggatccatc gagcctgctc ccgacaccgc
     3421 cgccggctgg ggcagccgcg ccgggttacc gatagggcgg aggttcacag gcgctcctcc
     3481 cacatggtcg ctgatcgcct gcaagcgctt caaaatcgat tgccattgct cccaatccag
     3541 ctgctgtaaa aatggccgca atccctggat gaacggatcc tcgagcagtg cctcgccgtc
     3601 cgattccggt atgaagaacc tgttcagccc gacgttcagc gcttcgctga tcttttccag
     3661 cgtgcccaag ctcggcgtca tctgcccgct ctcaatgcgc gagaggtagg accgcgacac
     3721 tttgctcttc gattgcagct ggctctgcgt catcccgcgg gattcccgca gctggcggat
     3781 gcgctggccg atgttctcca ccgtctgctg gttggcgaac ttatcggtcg acacttccac
     3841 gccctgctcc tccaacggtg gcgctggggg gatcaggaac tccagtctct gcggcaatgc
     3901 ccgcacacaa cggcggcaat tgcccgtgcg agtgcgatac tgcactaacc cgcacgtctt
     3961 gcagcggatc acttccctgt ccatctctaa tgagttcaaa gatgactctt tttccatatg
     4021 tgcggacacc ggtgctcctc gctgactttt gtccggcgtg cggataggag aggaacgtcg
     4081 gaacgttctt cgggggaagg cagatactgg cgcgagggag agtctctact aggtgtcgtg
     4141 tagtgtcaag atcatttttt caaatttccc attttttccg tgggactagc actcggttta
     4201 cgccagattt tcgcctcttt tcttccgtct tttctccttt cgcgccaaaa actcccaacc
     4261 ccttgtgtcc gctccttaag accaccacaa tcctcgtggc ctgattgcgt ccgtaacgcg
     4321 gcaggccgtg tgtcgtaagc gacaatcgtc gttctgttgc acatctcgac cgcagcagac
     4381 cgcaacgt
//
LOCUS       DASTPM010000128         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1301589, whole genome shotgun sequence.
ACCESSION   DASTPM010000128 DASTPM010000000
VERSION     DASTPM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301589"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1349)
                     /locus_tag="VFR42_02255"
     CDS             complement(222..1349)
                     /locus_tag="VFR42_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0368003.1"
                     /translation="MSAELLSRALVAKLRRSTLRPYEDGRRRLETDTCALLCLLTCLF
                     VSAAATTAVPSVDPGKSLRVYFVDVEGGQATLFVTPEGNSLLIDTGWPGNNGRDADRI
                     VAAAHDAGIQKIDYVLITHYHDDHVGGAPQLAAKIPVGTFIDHSENREPGAQKLYDAY
                     KVLLDSEKYKHIVAKPGDLLPIKGMRAEVISSDGAVISTPLPGAGQPNPTCNDFNPPD
                     DKTENPRSLGTLLTFGKLRILDLGDLTANKEKELMCPINRIGKIDIFIASHHGFNQSD
                     SAALVHGIAPRVAIVDNGEKKGGSPSVLDIIKSSPGIVGMWQLHYSQEAGDAHNAPAG
                     FIANLQGPDAGNYLKVMAQPDGSFAVFNSRTKETKTYSAPQ"
     gene            complement(1366..2196)
                     /locus_tag="VFR42_02260"
     CDS             complement(1366..2196)
                     /locus_tag="VFR42_02260"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0368004.1"
                     /translation="MTAARADDLFDLSGRTAIVTGTSRGLGQYLARALARAGADLVLT
                     SRKRDDLAAFAKEIEALGRHAVCLELDVRNEQSIEKFAADAEAACGHLDILVNNAGCN
                     VRKLALDVTWDDWNLILDTNLRGSFFVAQAVARRMIPRGYGRIINIGSVTSVAGYAGL
                     GPYGASRGGIRQLTMSLADDWGKHGITVNCLAPGWFRTAQNNVMYEDKEWVEYLTERI
                     PVKRPGQPNDLDGAVVFLASESSRYVTGQTLLVDGGISTGAMRALRQPASARSEPLRK
                     "
     gene            complement(2193..>3145)
                     /locus_tag="VFR42_02265"
     CDS             complement(2193..>3145)
                     /locus_tag="VFR42_02265"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEU0368005.1"
                     /translation="VAACGICGSDVHGYDGSSGRRIPPLVMGHEAAGTIEAVGREVKN
                     FAAADRVTFDSTVYCGECSNCRRGDINLCDRRQVLGVSCGDYRRAGAFAEFVAVPARI
                     LHRLPEALPFEEAAMLEAVAVAIHAVNLAEISANTTALVIGAGTIGILTMQALRAAGC
                     ARVFVTDVDQDRLEMARQLGATEILLSDESLNAKLLQLTDGEGVDMALEAVGRNETVA
                     AAIDGVRKGGGVVLVGNIAPNVNLPLQKVVTRQIRLQGSCASAGEYPQAIRWMASGTI
                     RVKPLITVVAPLEEGASWFERLYAREPNLLKVVLTPGTSA"
BASE COUNT          637 a         1015 c          860 g          633 t
ORIGIN      
        1 gtcggtataa atcgtgcgct ttgagcgttg caactcgatt gaaaccgcac ttactgagct
       61 ctgaaatcgc aaggtcggcc gaggacccga caggaacttt ctcacgaata ggcatttcgc
      121 actccgcgtt tgcaggcttc gcacatgaaa ctaggagcgt cacgacagac agcaagagcg
      181 caaccgccgt accctttaag cgtgctccga ccggttgagg tttactgcgg ggccgaatag
      241 gtcttggtct ccttagtccg cgagttgaaa acggcaaagc tcccatcagg ctgcgccatc
      301 accttcaagt agttgcctgc gtcggggcct tgcaggttag cgataaaccc tgcgggcgcg
      361 ttgtgggcgt cgcccgcttc ctgcgagtaa tgcagctgcc acattcccac aatccccggc
      421 gaagacttga tgatgtccaa cacggacggg cttccgcctt tcttctcgcc gttatccacg
      481 atcgcaactc gcggcgcgat gccgtggacc aacgcggcgc tgtcgctctg gttgaacccg
      541 tggtgggaag cgatgaagat gtcgattttc ccgatgcgat taatcggaca catcaactct
      601 ttctctttgt tcgcagtgag gtcacccaga tccaatattc tcagtttgcc aaacgtgagc
      661 aaagttccca gcgaacgcgg attctctgtc ttgtcgtcgg gaggattgaa atcattgcac
      721 gttggattgg gctgccctgc gccgggcaac ggtgtggaaa tgacggcgcc gtccgaggaa
      781 attacttccg cgcgcatccc tttgatcgga agcaaatcgc ctggcttggc gacgatgtgc
      841 ttgtattttt ccgagtcgag caggactttg taggcgtcgt aaagcttttg cgcgcccggt
      901 tcgcgatttt cactgtggtc gatgaacgtg ccgacgggaa ttttcgcagc aagctgcggc
      961 gcgccgccga cgtggtcgtc gtgatagtgc gtgatcagca cgtagtcgat tttctggatg
     1021 cccgcatcgt gtgctgcggc cacgattcgg tctgcatcgc gcccattgtt acccggccac
     1081 ccggtatcaa tcagcaggga attgccctcc ggcgtgacga acagcgtagc ctgcccacct
     1141 tctacgtcta caaaatagac gcgaagtgac ttgccggggt ccacggacgg taccgcggtt
     1201 gtggccgccg cagaaacaaa gaggcaggtg aggagacaga gaagtgcgca agtgtccgtc
     1261 tccaaacgcc gacgcccatc ttcgtagggg cgcagcgtgc tgcgccgcag cttggcaacc
     1321 aacgctcgtg acaaaagttc agcggacatt aggctttctc ccagactatt ttcgcaatgg
     1381 ctcggaccgt gccgaagctg gttggcgcaa cgctcgcatg gcccccgtgg aaatcccgcc
     1441 gtccaccagc agcgtctgcc cggtgacata ccggctagac tccgacgcca gaaaaaccac
     1501 cgcgccgtcg agatcgttcg gctgcccggg ccgcttcacc ggaattcgct ccgttaaata
     1561 ctccacccac tctttgtctt catacatcac attgttttgc gcggtgcgaa accatcccgg
     1621 cgctaggcaa ttcaccgtaa tcccgtgctt accccaatcg tcggccagac tcatcgtgag
     1681 ttgccggatc ccgccgcggc tcgctccata aggacccagc cccgcatatc ccgccacgct
     1741 cgtcaccgag ccaatgttga taatccgtcc gtagccgcgt ggaatcatgc gccgcgccac
     1801 cgcctgcgcc acaaaaaagc tgccccgcaa atttgtatca agaatcaaat tccagtcgtc
     1861 ccaggtgaca tccagcgcca gcttgcgcac gttgcatccc gcattgttca ccagaatatc
     1921 cagatggccg caggcagctt ccgcatcggc cgcaaacttt tcgatgcttt gctcgtttcg
     1981 cacatcaagt tcgagacata ccgcgtgccg cccaagcgct tcgatttctt tggcgaacgc
     2041 agcgaggtca tcgcgcttgc ggcttgtgag aacgaggtca gctccggccc tggccagcgc
     2101 tcgcgctaaa tattgcccca gcccgcgact cgttcccgta acgatcgcgg ttctcccgga
     2161 gagatcaaaa aggtcgtcag cgcgagccgc agtcatgcgg aagttcccgg agtgaggacg
     2221 actttgagga gattcggttc gcgcgcgtag agccgttcaa accagctcgc accttcctcc
     2281 agcggtgcga cgacggtaat cagcggcttc acacgaatcg tgccggaagc catccagcga
     2341 atcgcttgcg gatattcgcc ggccgaagcg caagaaccct gcaggcgaat ctgccgcgta
     2401 acaactttct gcaaaggaag attcacgttg ggcgcgatgt tgccgaccag tacgacgcct
     2461 ccgcctttgc ggacgccgtc aatcgcggcg gcgacggttt cgttgcgacc aacggcttcg
     2521 agcgccatat ccacaccttc gccatcggta agttgcagca gtttcgcgtt cagcgattcg
     2581 tcggaaagga gaatctccgt cgcccctagc tgccgcgcca tttccaggcg gtcttggtcc
     2641 acgtcggtga caaatacacg cgcgcacccg gccgcgcgaa gcgcctgcat cgtcagaatg
     2701 ccaatcgtgc cggcgccaat caccagcgcg gtggtgttgg cggaaatctc cgcaaggttc
     2761 accgcgtgaa tcgccaccgc aaccgcttcc agcatcgccg cttcctcaaa tggaagagct
     2821 tccggcaggc ggtgcagaat ccgcgccggc accgcaacaa actccgcaaa agctcccgcg
     2881 cgccgatagt ccccgcagga aactcccagc acctgtcgcc ggtcgcataa attgatatcg
     2941 ccgcgccggc agttggagca ctctccgcag tacaccgtcg aatcgaacgt aacacgatcc
     3001 gccgccgcaa aatttttcac ctcgcggccg accgcctcga tggtgccagc cgcttcatgc
     3061 cccatcacca gtggcgggat ccgccggccc gacgacccgt cgtacccgtg cacatcactg
     3121 ccacaaatcc cgcaagccgc caccc
//
LOCUS       DASTPM010000129         6163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1301614, whole genome shotgun sequence.
ACCESSION   DASTPM010000129 DASTPM010000000
VERSION     DASTPM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301614"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFR42_02270"
     CDS             complement(<1..541)
                     /locus_tag="VFR42_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEU0368006.1"
                     /translation="MFTGFVGVFFIAALTVPVFAQTKEQNRIENSGMVMGEILNIPDN
                     IPGGLLSKAECVVVIPSTLRFAFIVGGSYGRGVITCRSGQEFDGPWGAPGMMALEGAS
                     FGFQAGGQATDFVLLLMNPRAAGAILSSKVKLGVDASVAAGPVGRASEAALDVSMRAE
                     ILSYSRARGLFAGISLAGST"
     gene            903..2366
                     /locus_tag="VFR42_02275"
     CDS             903..2366
                     /locus_tag="VFR42_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368007.1"
                     /translation="MSSAAIVRLGTIKEVARFQEHLRALGVSIPCDSEIQHGDDSPLL
                     TTLQRGDLRIGNRIAINPMEGWDGTLDGRPSEHTLRRWRRFGQSGAKLIWGGEAVAVR
                     HEGRANPNQLVIAEHTREGLAQLRAVLIEEHKKVAGSDQGLLIGLQLTHSGRYCRPNA
                     DGRPEPRILYRHPILDRRLNLPADYPVLSDGEVRGIIEDFHRGARVAWELGFDFVDVK
                     HCHGYLGHEFLGGHTREGNYGGSFANRTRFLREVVEGIRAIAPGLKIGVRLSALDTIP
                     FRPDPAKSAPGKPGPGIPEEYEQLIPYRWGFGVNEQNPAEPDLAEGMQFLGVLEELQI
                     SLVNITAGSPYYNPHVQRPALYPPSDGYLPPEDPLVGVARQIQATRELKQRFPKLIVV
                     GTAYTYLQDFLPHVAQAALLEGWVDVVGIGRMTLAYPQILWDAANGRTVEHKRVCRTF
                     SDCTTAPRNGLPSGCYPLDGYYKGSEMAVKLAAIKKH"
     gene            2415..3371
                     /locus_tag="VFR42_02280"
     CDS             2415..3371
                     /locus_tag="VFR42_02280"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF017951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0368008.1"
                     /translation="MQVEEIRRRQKPRRKVQGISAALLPFEVDRRVAVAAFQKHLVAT
                     QRAGLANAVNMDTGYVNYLSDSEKDDVLRWTREALGKDVAFVAGAYIEGQDGDVVALY
                     RREMDRIVAHGGVPILFQTRRLHGKSAQEKAEVYRAACQGFPQVLAFELGTMFSPNGE
                     IFDEDTVRRLMDIPELKGMKHSSLDRSIELSRLGLRDAHRPDFRIYTGNDLGINMIEY
                     GSDYLLGLATFCPERFAERDRLWEAGEAAYYALSDALQHLGNVAFRAPVPAYKHSAAV
                     FLHLTGRIPSAQPHPKNPRRPEWEAEMLHDCARRLGYQAVFR"
     gene            complement(3392..3706)
                     /locus_tag="VFR42_02285"
     CDS             complement(3392..3706)
                     /locus_tag="VFR42_02285"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEU0368009.1"
                     /translation="MDEKQPQPQPGQMQIKADEKELQGLYSNLVMLQHNLEEFTLNFV
                     YIFPNGAQGKLLSSMIVSPGHAKRIWRALGENISRYEAQFGPIKEAPEGSNPTPNVGF
                     VQ"
     gene            3850..5232
                     /locus_tag="VFR42_02290"
     CDS             3850..5232
                     /locus_tag="VFR42_02290"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU0368010.1"
                     /translation="MAPPTEQLIEAEPTATATPLQSRAATTPVAQSPSLPEAHRTIPI
                     VSELSWFRKVLAFAGPGYLVAVGYMDPGNWATDLGGGAKFGYTLLSVILLSNLMAMLL
                     QGLSAKLGIVTGRDLAQACRESYSRPVSFVLWVICEIAIAACDLAEVLGSAVALKLLF
                     GLPLMAGVLITALDVLIVLALQGRGFRLIEAFVITLIASIAACFAYEIFFAQPLWREA
                     AAGFVPRLEILKNREMLYIAIGILGATVMPHNLYLHSSIVQTRAFATDASGKREALRF
                     AVFDSTLALTFALFINAAILILGAAAFHTRGLHDVAEISDAYKLLTPVLGASLASTLF
                     ACALLASGQNSTLTGTLAGQIVMEGFLNLRLKPWLRRLSTRALAIIPAIIVIGIYGEG
                     KVTSLLILSQVVLSFQLPFAVIPLVQFTSDKVKMGGFANSKLTAVIAWVVAIAIVVFN
                     AELLYLTFRS"
     gene            5250..5693
                     /locus_tag="VFR42_02295"
     CDS             5250..5693
                     /locus_tag="VFR42_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0368011.1"
                     /translation="MPSPGRKKLAGTIFTVLLLVAAGSAVYAFFHPGPWIVPEEAKRM
                     QNPLKRSQANLPAAQRLYLDKCAECHGDSGKGDGSQAKMYEPRPTDFTDVAHMHSVSD
                     GELFYKISEGHRPMPGFKKRFTEEQRWQLVLFLRSLAAQSDPHAS"
     gene            5805..>6163
                     /locus_tag="VFR42_02300"
     CDS             5805..>6163
                     /locus_tag="VFR42_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0368012.1"
                     /translation="MRRLTFVLSMWLLVTGVAAAAPGAVRCGKLLDVRSGRLLSDQVV
                     TFDADGVITSVAPASGTSAAAIDLSQATCLPGLIDVHTHITGDPTHSGYAGLGISVPR
                     SAVTGAKNARLTLRAGFT"
BASE COUNT         1225 a         1584 c         2019 g         1335 t
ORIGIN      
        1 aggtggagcc tgcaagcgag attccagcga acaggccgcg ggctcgtgag taagaaagaa
       61 tctcagcacg cattgagaca tcaagtgccg cctcggaggc gcgaccgact ggacccgccg
      121 caaccgaggc gtccacacct agcttgacct tgcttgagag aatggcgcca gcggcgcgtg
      181 gattcatgag aagaaggacg aaatccgtgg cttggccgcc agcctgaaat ccaaagctgg
      241 cgccttctag cgccatcata ccgggggcgc cccagggccc gtcgaattcc tggcccgacc
      301 gacaagtgat gacgccacgg ccgtagcttc cgccgacgat gaaagcgaaa cgcagggtgg
      361 aaggaatgac cacaacgcac tctgccttgc tgagaagacc gccaggaatg ttgtccggga
      421 tattgaggat ttcgcccatc accatgccgg aattttcgat gcgattctgc tccttggttt
      481 gagcaaaaac gggcacggta agagcagcga taaagaaaac tcccacgaac cctgtaaaca
      541 ttgcgccggg acgcggaagc gcggtgcaaa ttgattggag aagttggcgg agtaccgaag
      601 cagaccggta ggcgcggatg ctgcgaaagc ggaacggttg cgtcatggaa ggctctttct
      661 tacaggggga gataaggcgc gtggaggtct atattcgtgt tccggagcgt tggtgtcaag
      721 aaattgagca cgcccggaga gcctaccaca ccgacaggta ggcagggacg acttgccggg
      781 acgtgcggaa tcctgcaaac tgcacggtca tgagacttgg aacctgcata cctgcgcgaa
      841 gtctttgttg atcctgtgac cgaaccgagc cttgttatcg aaatgcggat tgaacaaacg
      901 gcatgagttc cgctgcgatt gtgcggcttg gaacgatcaa agaggtggcg cggttccagg
      961 agcatctgcg cgcactcggg gtgagtattc cctgcgacag cgaaattcag cacggggatg
     1021 attcgccgct gctgacgacg ctgcaacgcg gggatttgcg cattggcaat cgcattgcga
     1081 tcaatccgat ggaaggctgg gacggcacgc tggatgggag gccgagcgaa catacgctcc
     1141 ggcgctggcg gcgttttggt cagagcggcg cgaagctgat ctggggcggg gaggccgtgg
     1201 cggttcgaca tgaggggcgc gcgaatccga atcaactggt gattgccgag catacgcgcg
     1261 aagggttggc gcagttgcgc gccgtgttga tcgaggagca caagaaagta gccggttcgg
     1321 accaagggtt gctgattggg ctgcagttga cgcactccgg acgctattgc cggccgaatg
     1381 cggacggacg gccggagccg cgcattttgt atcggcaccc gattttggac cggcggttga
     1441 atttgcccgc agactatccg gtgctgagcg atggagaggt ccgcgggatt attgaggatt
     1501 ttcaccgcgg cgcgcgcgtg gcctgggagt taggatttga ttttgtggat gtgaagcact
     1561 gccacggcta tttgggccat gagtttctgg gcgggcacac gcgggaaggg aactacggtg
     1621 gaagttttgc gaatcgcact cggtttttgc gggaagtggt ggaagggatt cgcgcgattg
     1681 ctccggggtt gaagatcgga gtgcggctgt cggcgctcga cacgattccg ttccggccgg
     1741 atcccgcgaa atcggcgccg ggcaagcctg ggccggggat tcccgaagag tacgagcaac
     1801 tgattccgta ccggtgggga ttcggcgtga acgagcagaa tcctgccgag cctgatctag
     1861 ccgaagggat gcaatttctg ggtgtgctgg aggagttgca aatttcgctg gtgaatatca
     1921 cggcagggag tccttattac aatccgcacg tacagcggcc ggcattgtat ccgccttcgg
     1981 atggatattt gcctcccgaa gatccattgg tgggcgtggc gcggcagata caggctacgc
     2041 gagagctgaa acagcggttc ccgaaattga ttgttgtggg tacggcgtac acctatttgc
     2101 aggattttct gccccatgta gcgcaagctg cgctgctaga ggggtgggtg gatgtggtgg
     2161 gaattgggcg tatgactctt gcttatccgc aaattctgtg ggacgcggcg aatgggcgta
     2221 cggtggaaca caagcgggtt tgtagaacgt tcagcgattg caccacagcg ccgcggaacg
     2281 gattgccttc ggggtgttat ccgctggacg ggtattacaa gggttcggag atggcggtga
     2341 agctggcggc gatcaagaag cactgacggc aattctttgt tgcggtgcgc gagacacgga
     2401 ctttgagggg atagatgcag gttgaagaaa taaggcggcg gcaaaaaccg cggcgcaaag
     2461 tgcagggaat ttcggcagca ttgttgccgt tcgaagtgga ccgacgagtc gcggttgcgg
     2521 cttttcaaaa acatctcgtg gcgacgcagc gggctggcct ggcgaacgca gtcaacatgg
     2581 acactgggta tgtgaactac ctgagcgact cggagaaaga tgacgtgctg cgctggacgc
     2641 gcgaggcgtt gggcaaggac gttgcgtttg tggcgggagc gtatatcgaa gggcaagacg
     2701 gcgacgtggt cgcgttgtat cggcgggaga tggaccgcat tgtcgctcat ggtggtgtac
     2761 cgatactttt tcaaacgcgg cgactgcacg gaaaatcggc gcaagagaag gctgaggtgt
     2821 atcgcgcggc gtgccagggg ttcccgcaag tgctggcgtt cgaattgggg acgatgttct
     2881 cgccgaacgg cgagattttt gacgaagaca cggtgcggcg gttaatggac attcccgagt
     2941 tgaagggaat gaagcattct tcgctggacc gctcaattga attgagccgg cttggtttgc
     3001 gggacgcgca tcgcccggac tttcgcattt acaccgggaa tgatctgggc atcaacatga
     3061 tcgaatatgg ctcggactac ctgctggggc tggcgacgtt ttgcccggag cgattcgcgg
     3121 agcgggatcg attgtgggaa gccggggagg cggcgtatta cgcgctgtcc gatgcgctgc
     3181 agcacctggg caacgttgcg tttcgcgcgc cggtgccggc gtacaaacat tccgcggcag
     3241 tgtttttgca tttgacggga cgcattccga gcgcgcagcc gcatcccaaa aatcccagac
     3301 ggccggagtg ggaagcggag atgttgcacg actgcgcgcg gaggctgggc taccaagcgg
     3361 tatttcgcta agaaacgttt cgagtacggc gctactgcac aaagccgacg ttgggagtag
     3421 gattgctgcc ttcgggcgct tccttgatag ggccgaactg tgcttcgtat cgggagatat
     3481 tttcgccgag cgcgcgccag atgcgtttgg catggccggg gctgacgatc atgctgctga
     3541 gcagcttgcc ctgcgcgccg ttggggaaaa tgtaaacaaa attcagggtg aactcttcca
     3601 gattgtgctg gagcattacc agattgctgt aaaggccctg caattccttt tcgtcagcct
     3661 tgatttgcat ttggcctggc tgcggttgcg gctgcttctc gtccatgcgt gatctcctaa
     3721 ggttctttcc aaaggtacca cagaggtacg ggggagtagt ttctccggcg gcacttcagg
     3781 aaaaagacct attgacacca ggctgcatta aagatagttt agggatgcta ttaaccatag
     3841 ttaataagaa tggcgccccc gacggaacaa cttatcgaag cggaacccac cgcgactgca
     3901 acgcccttgc agtcgcgagc cgcaactact cccgtagcac agtcgccctc gcttcccgag
     3961 gcgcatcgca cgatccccat cgtttctgag ctcagctggt ttagaaaagt gctggcgttc
     4021 gcaggtcccg gctacctggt ggccgtcggg tatatggacc cgggcaactg ggcaacggac
     4081 ctcggtggcg gagcaaagtt cggctacacg ctgctgagcg tgattttgct gagcaatctg
     4141 atggcgatgc tgctgcaggg gctttcggcg aagctcggaa tcgttacggg gcgcgacctg
     4201 gctcaggcgt gccgggaatc gtactcacgg ccggtttcgt ttgtgctgtg ggtgatttgc
     4261 gaaatcgcga ttgcggcgtg cgatttggcg gaggtgctgg ggtcggcagt ggcactgaag
     4321 ctcctttttg gcctgccatt gatggccggc gtgctgatca ccgcgctgga cgtgctgatt
     4381 gttctggcgc tgcaggggcg cggattccgg ctgatcgagg ctttcgtgat taccctgatc
     4441 gcgagcatcg cggcgtgttt tgcttacgag atttttttcg cgcagccgct ttggcgcgag
     4501 gctgcggccg gctttgtgcc gcgactggaa atcctgaaga accgcgaaat gctttacatc
     4561 gcgattggaa tcctgggcgc gacggtgatg ccgcacaatc tctatctgca ttcgagtatt
     4621 gtgcagacgc gcgcttttgc cacggatgct tcggggaaac gcgaagcact gcgattcgcc
     4681 gtcttcgatt ccacgctggc gctgacattc gcgctcttta tcaatgccgc gattttgatt
     4741 ctgggcgcgg cggcgtttca cacgcgtggc ttgcatgacg tagcggagat ttccgacgcg
     4801 tacaagttac tgacaccggt tttgggagcg agcttggcta gcacgttgtt tgcctgcgcg
     4861 ctgctggcgt cggggcaaaa ctcgactttg acgggcacgc tggccggcca gatagtgatg
     4921 gaaggatttc tgaatttgcg gttaaagccc tggttgcggc ggttgagcac gcgcgcactg
     4981 gcgattattc ccgcgattat cgtgattggg atctatggtg aaggaaaagt gacctcgctg
     5041 ctgatcctca gccaggtggt gctgagcttt cagctgccgt tcgcggtgat tccgcttgtt
     5101 caattcacca gcgacaaggt gaagatgggc ggcttcgcca actcaaagct cacagctgtt
     5161 atcgcgtggg tcgtggcgat cgcgatcgtt gtcttcaacg ccgaactgct ttacctcacc
     5221 ttccgcagct gacaaaatca ttttcggaca tgccttcacc cggccgcaag aagctagctg
     5281 gtacgatctt cactgttctg ctcctggttg ccgcgggctc ggcggtgtat gcgtttttcc
     5341 atccgggacc gtggattgtg ccggaagaag ccaagcgaat gcagaatccg ctgaaacgct
     5401 cgcaggcgaa tcttcccgcg gcgcagagac tgtatctgga taaatgcgct gagtgccacg
     5461 gcgactccgg caaaggcgat ggctctcagg ccaaaatgta cgagccacgg ccgacggatt
     5521 tcacagacgt ggcgcatatg cattcggtca gcgacgggga attgttctac aagattagcg
     5581 aagggcaccg gcctatgccc ggcttcaaga aacgcttcac ggaagagcag cggtggcagt
     5641 tagtactgtt cttgcgctct ctggcggcgc aatcagatcc ccatgcaagc tagccgggag
     5701 gggcagcacc cctcactcac cgccaactcc gattcgttgg ccctccagtg ttttcgcgct
     5761 attctcgttg cgcacttttt tgattccaac ggcttgaggt attcatgcgc agacttactt
     5821 ttgttctgag tatgtggttg ctagtgacgg gcgtcgcggc ggcggcgccg ggagcggtgc
     5881 gctgcggcaa attgctggac gttcgctctg gaagactcct ttccgatcaa gtggtcacat
     5941 ttgacgcgga cggagttatt acttcggtgg ctcccgcctc cggaacaagc gcggctgcca
     6001 tcgacctgtc gcaggctact tgcctgccgg gcttgatcga tgtccacacc cacattaccg
     6061 gggatccgac ccacagcggc tacgcgggat tggggatttc cgtgccgcgg tccgctgtta
     6121 ccggcgcaaa aaatgcgcgg ctgacattgc gcgcgggatt cac
//
LOCUS       DASTPM010000130         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_465500, whole genome shotgun sequence.
ACCESSION   DASTPM010000130 DASTPM010000000
VERSION     DASTPM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465500"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1673)
                     /locus_tag="VFR42_02305"
     CDS             complement(<1..1673)
                     /locus_tag="VFR42_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU0368013.1"
                     /translation="MLKIPSKAQLPLRLFAMAISAGLFVYLIRRAGPSKLWTSLSELG
                     WGIILVIALAGVSHLVRTWGWRLTLGDDQHKISFSRLVGLRLGAEAAGQLGILGQTLG
                     DSVRVSRMSAEIPIAGGLASVTLDRGLYIVTGIVTTIAGILAALPLVALSHALRLYAS
                     LFAFALIAFLILAVLAVRKRWPVLSGSARLIGCVPSLKNWMESKYLLLQSVENALFDF
                     HHKTPGAFWSSFSLNLAAQCMAVSEVCLILWLMGVKMGFFSALVIEALTKLVNVLGNF
                     NPGNIGTYEGGTMLIGKMFGLSSATGLALGVSRRLRSFFWAAVGGICFVLLTPRKHRG
                     SKIPASTTVTVATSPRVRANSSSNRSPENEIVFAIFLAEGEASGSQFSSSLSRVGTLP
                     ILLRTILAAQKAEAARIMVVADPTIKRKVRRALLVTGRLPESVEWIDAAGGASLSQRL
                     LLIANKVPSERLVLIDGNTTYHPSLLKKASEWNNEGTALALTSGDKLVGIHALSVEMI
                     RDFAERCPTQAGSLEDLHASLIEMHSVACMPVPEHLWQRVSTPEDRQSA"
     gene            complement(1666..2427)
                     /locus_tag="VFR42_02310"
     CDS             complement(1666..2427)
                     /locus_tag="VFR42_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU0368014.1"
                     /translation="MTTVNECLILAAGNGSRIASVAAGVPKPLVPLRGIPLLEHVMTS
                     CQEAGITRFVIVVGYRADQIRRWFVERSFDNISVTLVENLDYHKANGVSALAAKDELR
                     NPFLLLMSDHIFEPKTAKALVEQPLVDGEVILAVDHNLDGVFDLDDATKVKCETDHIV
                     DIGKDLARYDALDTGMFLCSPALFLRLESAKKDGDCSLSDGMRQLAREQKLKAFDIGD
                     AHWQDVDSPEALAHAESVFDLNFCENPFAESFVDA"
     gene            complement(2497..3240)
                     /locus_tag="VFR42_02315"
     CDS             complement(2497..3240)
                     /locus_tag="VFR42_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HEU0368015.1"
                     /translation="MKYHFLLTVLFLLPASSVIAADGPSALPTAEEIVRQMGAHDQQR
                     QASMEGYAGKRRYILENHHFQKRAEMLVQVQGDRDGTKHFEVMSEDGWKAARKHVLRK
                     MLESETETSRPEMRARARLIPENYDFEVIGTELVSDRTAYVLAITPKRKEKYLFRGRI
                     WVDAEDYALVRAEGNPAKNPSFWTKNTHFVQSYQKNGPLWFPLSTQSVTEAHIFGTTD
                     VNIEYFDYAPKTSHHADNSVMTAMEMPKQ"
BASE COUNT          819 a          989 c         1010 g          800 t
ORIGIN      
        1 tcggcagact ggcggtcttc gggagtgctc acgcgttgcc acaagtgctc cggcaccggc
       61 atgcaagcga ccgagtgcat ctcgatgagt gaagcgtgaa ggtcctcgag gctgccagct
      121 tgcgttgggc aacgttctgc gaaatcgcgg atcatctcga cagagagcgc gtgaatgccg
      181 acaagtttgt cgccactcgt cagcgccaac gccgtgccct cgttattcca ctcactggct
      241 ttcttgagta gcgatggatg atacgtcgtg tttccgtcga tcagaaccaa gcgttcgctg
      301 ggcaccttat tggcaatgag caataatcgt tgggaaagag aggcgccacc ggctgcatcg
      361 atccactcga cagattcggg aagtcgcccg gtaacgagca aggcgcgccg cacttttcgc
      421 ttgatggtcg gatcggccac caccatgatg cgcgcagcct ccgccttttg cgcagcgagt
      481 atcgttcgga gaaggatggg cagagtgccg actcgcgaga gtgaagagct gaattggctg
      541 ccactcgcct ctccttcggc gaggaaaatg gcgaacacga tttcgttttc cggtgaacgg
      601 ttgcttgaag aattcgctcg caccctcgga gatgtcgcga ccgttacggt tgtgcttgca
      661 gggattttag atccgcggtg ctttcttggc gttagcaaga cgaaacaaat gccgccaacg
      721 gcggcccaga aaaaggaccg caagcgtctt gatactccca gagccaatcc ggtcgcgctg
      781 ctcaggccaa acatcttgcc aatcagcatg gttccacctt cgtaggttcc aatgttgcct
      841 gggttgaaat tgcccaggac gttcacaagc ttggtcaatg cttcgatgac caaggcactg
      901 aagaagccca tcttcacgcc catcagccag aggatcaaac acacctccga tacagccatg
      961 cactgagcgg ccaaatttag agaaaaactt gaccaaaatg ctccgggagt cttgtgatgg
     1021 aaatcgaaca gggcattctc aacggactgc agtagcagat atttgctttc catccaattt
     1081 ttgagggacg gaacgcagcc gatcaagcgg gcgctccccg aaagcaccgg ccaacgtttt
     1141 cgtaccgcca acaccgccaa tattagaaag gcgattagtg caaaagcgaa cagactcgca
     1201 tacaggcgca gagcatggga aagcgccacg aggggcagag cggccaagat ccccgcgata
     1261 gtggtcacaa tccccgttac gatgtagagg ccgcgatcga gagtcaccga ggcgagacca
     1321 ccggcaatgg gtatttctgc actcatgcgc gagactcgga cagaatctcc aagcgtctgc
     1381 cccaggatcc cgagttgtcc ggcggcttcc gctcctagtc gaagccccac caaacgcgaa
     1441 aaggagatct tatgctggtc gtcaccgagt gtaagccgcc agccccaggt cctcacaagg
     1501 tgcgagactc cggccagggc aatcactaaa atgattcccc aaccaagttc agaaaggctt
     1561 gtccatagct tgctgggacc agctcgccta ataaggtaaa caaacaagcc tgcgcttatc
     1621 gccatggcga agaggcgaag cgggagttgt gctttcgatg ggattttaag catcgacaaa
     1681 actttcagcg aatggatttt cacagaaatt cagatcaaag acgctttcgg cgtgagccaa
     1741 tgcttcgggg ctgtcgacgt cctgccagtg cgcgtcgcca atatcaaacg ccttcagttt
     1801 ttgctctctg gccagttggc gcatgccgtc cgacagcgag cagtctccat ctttcttcgc
     1861 tgattcgagt ctgaggaaga gcgccgggct gcacaagaac atccctgtgt cgagtgcgtc
     1921 ataacgggca agatctttgc cgatatcaac gatatgatcc gtttcgcatt tgaccttcgt
     1981 tgcgtcgtcg aggtcaaaga caccatccag attgtgatcg accgccagaa tgacctcgcc
     2041 gtcgacgagt ggctgctcca ctagcgcctt ggccgttttt ggctcgaaaa tgtggtccga
     2101 cattagaagt aagaacggat tgcgaagttc atctttggcc gccagggcag agaccccgtt
     2161 ggctttgtgg tagtccaggt tttcgaccaa cgtgacggaa atgttgtcaa atgaccgctc
     2221 gacaaaccag cggcggatct ggtcggcgcg atacccgacg acaatcacaa atctcgtaat
     2281 tccggcttcc tgacacgacg tcatgacgtg ttcgagaagt ggtatgcctc ggaggggcac
     2341 aagaggcttg ggtacgccgg ccgccacaga tgcaatccgg ctcccgttgc ccgcagcgag
     2401 aattaggcac tcattaacgg ttgtcatggc ccaccttagt gaatcggtgt ttttcgcaaa
     2461 gaagctctgt ggtccaaagg atggtctttg tctgtttcat tgcttcggca tctccattgc
     2521 ggtcataacg gagttgtcgg catggtggga cgttttgggc gcgtagtcga aatactcgat
     2581 gttcacgtcc gtggtgccaa aaatgtgagc ttcggtgacg ctctgggtcg agagcggaaa
     2641 ccagagagga ccgttcttct ggtagctctg cacgaaatgg gtgtttttcg tccagaacga
     2701 ggggttcttg gcagggttgc cttccgcgcg caccagcgcg tagtcctcag cgtctaccca
     2761 gatgcgccct cggaataggt atttctcctt ccgtttggga gtaattgcca gaacgtaggc
     2821 cgtgcgatcg gacaccagtt ccgttccgat cacttcaaag tcatagtttt cgggaatgag
     2881 tcttgccctg gcacgcatct cggggcgcga agtttctgtt tccgattcga gcattttgcg
     2941 caaaacatgc ttgcgcgctg ctttccatcc atcttcggac atgacctcga agtgctttgt
     3001 gccgtcccgg tcaccttgca cctgcacgag catttcggca cgtttttgga aatgatggtt
     3061 ttcgaggatg taccgccgtt ttccggcgta tccctccatg gaggcctgcc gttgctggtc
     3121 atgggcgccc atctgcctca cgatttcttc agcagttgga agcgcgctgg gaccgtccgc
     3181 cgcaatgaca gaagaggcgg gaagcagaaa caaaacggtg agcaggaagt gatatttcat
     3241 cgtcaaaact ccaaaacaaa ttttccgcgg tattcacgcc aaaactcgtc tgcgttccat
     3301 tccaccgaca atgagaaggg caacacgaat gccaaacttg ttatgcgttg gcacaacgga
     3361 acttcttgaa ttgtcttgag ataaacgcca agtaagagtt tcgtgagttg agtgcccgtc
     3421 tgctgctgcc gcagtggcgg caaggtgccc gccttggggc acaaggcgaa agcgacaaac
     3481 acgccacacc tcggcgctgc ttgattgaaa tgaacatggt tgatggacgt ggcgggactc
     3541 gaacccgcgg caccctgctt gcaaagcgtg aaccgccctg cctgacggtt gcgggctgat
     3601 tgcgataagc gtcgcaga
//
LOCUS       DASTPM010000131         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_234857, whole genome shotgun sequence.
ACCESSION   DASTPM010000131 DASTPM010000000
VERSION     DASTPM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234857"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="VFR42_02320"
     CDS             complement(<1..1738)
                     /locus_tag="VFR42_02320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0368016.1"
                     /translation="MSALPVSIGPYRILAQLGEGSMGVVLKAQDQRLDRTVALKLIRA
                     RTDDPAKRQRLWQEARAAAQVAHPNACRLYDITEESGELVLVMEFVEGESLAQRLVQG
                     PLPAREAAQIALEVLSALQAFHKLGIVHRDLKPANILLSPHGAKLLDFGIVKQVLVPE
                     SDETAATLSDTTVAGVFLGTPRYASPEQFNGRAVDVRSDIFSLGAILFEMLTGRFAFS
                     GETFAELAHSVLHVTPPALTGSPAITAIGRIVHSALSRAPHERYLSAEKMAEDLRKAL
                     LAEGIDTVARAQTLRRMIVLPFRLVRRSEELEFLTYSLPESITVSLSGLENLVVRSSI
                     IAARYAQDVPDFQKIASEAEVDVILTGALLPVGEQLRITAQLVQVPDGAVLWSHSSEA
                     TVKELLQLHDTLVDRVVQEVLPQLSPHDQAVLHHDRPASPTVYQLYLRANECSREWEN
                     LPAAIQLYEDCVRLDPRYAPAWARLGRARWLCDKYTFGSAEGLRSADDAFQKAFELSP
                     DLPLAQHFYTYLQVDQGRSLQALQRLLDQSSRRHSDPELFAALAHVCRYCGLLQPALF
                     AHHEARRLDPQVP"
     gene            complement(1826..2047)
                     /locus_tag="VFR42_02325"
     CDS             complement(1826..2047)
                     /locus_tag="VFR42_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0368017.1"
                     /translation="MPIYEYICDDCKSQFEKIVINRQQEISCPKCSSKKATIQLSVFS
                     SAIAGGSSKSGSGSSGSGGACCGGGCGCH"
BASE COUNT          522 a          609 c          881 g          449 t
ORIGIN      
        1 tgggcacctg gggatcgagg cgacgcgctt cgtggtgcgc gaaaagagcg ggctgaagga
       61 gaccgcagta acggcagacg tgggctaaag cagcgaagag ttcgggatca ctgtgacggc
      121 gggaagattg atcgagcagg cgctgcaaag cctgcaggct gcggccttga tcgacctgaa
      181 ggtaggtgta gaagtgctga gcgagcggaa ggtccgggct tagctcaaag gctttctgga
      241 aagcatcatc ggcggagcgc aggccttcgg cagagccgaa agtgtatttg tcgcagagcc
      301 aacgagcgcg cccgaggcga gcccaagccg gggcgtaacg cgggtcgagc cgcacgcagt
      361 cttcatagag ctgaatggcg gcggggagat tttcccattc gcgggaacat tcgttggcgc
      421 gaaggtaaag ctgatagacg gtggggctgg ccgggcggtc atgatgaagc accgcctgat
      481 cgtgcggcga gagctggggc aggacttcct ggacgacgcg gtcgaccagc gtgtcatgga
      541 gttgcagaag ttccttcact gtggcttccg aggaatggga ccagagcaca gcaccatcag
      601 gaacttgcac cagttgggcg gtgatacgca gctgttcgcc aacaggcaag agcgcgccgg
      661 tgagaatgac gtcgacttcg gcttcgctgg cgattttctg gaagtcgggc acgtcttgcg
      721 catatcgcgc ggcaatgatg gacgagcgca cgacgagatt ttccagcccg gaaagcgaaa
      781 cggtgatgga ttccgggagg ctataggtga ggaattcgag ttcttcgctg cggcgcacca
      841 ggcggaacgg gagaacgatc atgcggcgga gggtttgcgc gcgggcgacg gtgtcaatgc
      901 cctcggcaag aagagctttg cggagatctt cggccatttt ctcggcgctc agatagcgct
      961 cgtggggagc gcgagaaaga gcggaatgaa cgatgcgacc gatggcagtg atggcaggag
     1021 aaccggtgag agcgggagga gtgacgtgga gaacggagtg ggcgagttca gcgaaggttt
     1081 cgccggaaaa agcgaaccta ccggtgagca tttcgaacag gatggcgccg agcgaaaaaa
     1141 tgtcggagcg gacatcgacg gcgcggccgt tgaattgttc gggcgaggcg tagcggggag
     1201 tgccgagaaa aacgccggcg acggtggtgt cgctgagggt ggcagcggtt tcgtccgatt
     1261 cgggcacaag tacttgcttg acgatcccga agtcgagcag cttggcgccg tggggcgaaa
     1321 gcaagatgtt ggcgggcttc aaatcgcggt gcacgatgcc gagtttgtga aaggcctgca
     1381 aggcggagag cacttcgaga gcaatctggg cggcttcgcg agcgggaagc ggcccctgga
     1441 cgaggcgctg ggcaagcgat tcgccttcga caaactccat gaccaggacc agctcgccgg
     1501 attcttcggt gatgtcgtaa aggcggcagg catttgggtg ggcaacctga gcggcggcgc
     1561 gcgcttcctg ccacaagcgc tggcgcttgg cgggatcgtc ggtccgggcg cggatgagct
     1621 taagagcgac ggtgcggtcg aggcgctggt cctgcgcctt tagaacgaca cccatgctgc
     1681 cctcgcccaa ctgggcgagg atgcggtaag gaccaatgga cactgggaga gccgacactc
     1741 aacacactca atctacggtg ggatttagga taccggagtc agcggcaggc tgccatggcg
     1801 ttgaaatgcg aaagtgcggg aaaggctagt ggcagccgca accgccgccg cagcaggcgc
     1861 cgccgctgcc tgaggagccg gagccggact tagaggagcc gccggcgatg gcggagctga
     1921 agacggaaag ctggatggtg gcttttttgc tggaacactt cgggcaggag atttcctgct
     1981 ggcggttgat gacgattttt tcgaattgag atttgcagtc gtcgcagatg tattcgtaga
     2041 tgggcatgta attttcctac tctaagatac cgcagaaagt tctccagcgt catctccgcc
     2101 tgggcgcaga ctgctcatta gaatcggctg aggcaaatcg aaacggtcgt gtctgggatg
     2161 gcgtcgcggg ccatttcacg agcttcctcg agcggccgta ggtgatgacg ccgggcaaaa
     2221 gtggaacggt cacctgatag cccggataga agggattacg cgcccggcga attttctttc
     2281 cgttccttcg agctgttacg acctgaatga gaggttcgaa gacaaccgta aagccgtatt
     2341 cgcgggattg ttcctcttcg gcaattgcga ctcgcggcgt gggacaagtc tagccgattt
     2401 gtggggcgaa cgcgcaacgt cacgacatgt accagccgcc gttgaggttt agggtgtggc
     2461 c
//
LOCUS       DASTPM010000132         9134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_61860, whole genome shotgun sequence.
ACCESSION   DASTPM010000132 DASTPM010000000
VERSION     DASTPM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61860"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFR42_02330"
     CDS             <1..849
                     /locus_tag="VFR42_02330"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU0368018.1"
                     /translation="ITGREENLTHLKGNGKFSFARADVSDQLNVAGDVAYVLHFASPA
                     SPPDYLRHPIETMRVGSVGTQNALELARAKGAKFFLASTSECYGDPEISPQHEEYWGH
                     VNSVGPRSVYDEAKRFSEALTMGYHRHYGVDTHIVRIFNTYGPRMRLNDGRALPNFVF
                     QALSEKPITVYGDGKQTRSFCYVSDLIDGIFRLAMSEEHLPVNVGNPEEITLLEFAQR
                     VRGHFPKAGPIVFEPLPQDDPRRRCPDIGKAKRLLGWEPKVALSDGLRKTIEYFQEQF
                     AAEKTA"
     gene            complement(861..2213)
                     /locus_tag="VFR42_02335"
     CDS             complement(861..2213)
                     /locus_tag="VFR42_02335"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU0368019.1"
                     /translation="MPLRLSVIGCGYVGLVTGACLAEAGHEVICTDIDAARIAQLKAG
                     QVPIYEHHLDKILVSAGNSGKISYTANAGEAIRFGEAIFICVGTPPRPDGSADLSAID
                     HVAQQIAAEARSPKLVVEKSTVPARTGIELQRVLSAYSRGGNGLRVASNPEFLREGTA
                     VEDFFHPDRIVVGVEDQAAADQLREIYRPILERKFTCPVHAGKCPDMQKAEFLVTTIS
                     SAELIKHASNSFLALKISYANAVSDLCEKLGANVEEVTHAMGLDPRIGTQFLRAGLGF
                     GGFCFPKDIQAFISLAATVGVDFEILKAAERVNKQRIDRFLDKISKALWVVKGKRLAI
                     LGLAFKANTDDIRFAPALDVLRRLLEEGAEVHACDPEALEKAKKQFPQAHFHEDPYRA
                     LEGAHAALVCTEWDEYRKLDWEKAGKVMARKLIVDGRNLYSPAIIKALGFEYVSFGRE
                     "
     gene            complement(2293..3462)
                     /locus_tag="VFR42_02340"
     CDS             complement(2293..3462)
                     /locus_tag="VFR42_02340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0368020.1"
                     /translation="MYEDQPDKTKGSVIVVGSSAAGLLTAAKVAAGGCDVRVLDAKCG
                     LDPAPRSLIVTHHFREQLREVAASSIVNEIRRFELFTDGRSAEVALNKPDLIIERARL
                     IPSLAREAGKAGAKISFDTRFLGLGPNSRGLRLKVESGGRREELHADHVVGADGAATR
                     VGRCAGWPPVETVPLVQAIVKLPKDCRADTTRVWFVPDDTPYFYWLIPENESRGALGI
                     IGEGSGQETKRRIERFLEKKNMQPMGWQGARIPVYRKWIPVERRVGNGKVFLVGDAAA
                     QVKVSTVGGIVTGFRGAQGVADALLRGNGNGELRALRRELATHWLIRKTLHHFEQEHY
                     SYLVDLLNSATRESLGEIHRDHSTRLLWNVLRRQPRLILLGLRGLLMGVRPRSSF"
     gene            complement(3642..4697)
                     /locus_tag="VFR42_02345"
     CDS             complement(3642..4697)
                     /locus_tag="VFR42_02345"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU0368021.1"
                     /translation="MAAGLLAFLIALLASLTLTMPVRAFALRVGMVDRPGPRKVHLQP
                     IPLLGGLAIYGGVALSVLFLVQGAARDQVWGILAGATLIAAVGILDDRGLLHHQIKLF
                     VGMPLAAVILLLSGIRAQIFSTLVGGRTGDVLDAALTIIWVVGITASFSILDHMDGLC
                     AGVAAMAAAFFALLAYLNGQVLVTVLAAAVLGAACGFLRWNFKPAKIFMGDGGAMFLG
                     FLMATLALKLRFAQPNLVSAWLVPILILGATIFDTTLVTISRSRRGLLPFATPGKDHA
                     AHRLANMGLGQRGAVLTMYMIGAVSGALALLVSIMPISFAIVAGVFAVAAALAGVAYL
                     ERAPYERQVPKPAAATD"
     gene            complement(4986..6197)
                     /locus_tag="VFR42_02350"
     CDS             complement(4986..6197)
                     /locus_tag="VFR42_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0368022.1"
                     /translation="MYNLSVRILLLNLYYPPDTSATAKMAETVVNVLAKNNDVTILCG
                     RPSYDPTERRPWRLFQTEGTANRQVVRVGSTDFPRLSMKRRVLNYLTYTALVIPRALF
                     TPCDAVLAMTDPPFEGIIGAFVAMLKRRPYFYNIRDMYPDMAVGGSIVKPGVLARVWE
                     KLHRWALRRATTVIVLGEDMRKRILAKGVRRERIEIVRDGAEIVPADSPQPQLDPEVI
                     QTIRGDFRFVLLHAGNLGFYGAWSTLVTAAKQLASDGIGFVFVGEGAQRAEIEVLADG
                     VGNIRFLPFFPSNKVPSVLAAADAHLITVRRGLEGVVVPSKMYGILAAGKPIVAVAPW
                     ETDAVALGGEFGFGVGADPDRPEEVASVIRALARAPERLAQMAGAARQAAPAYDRANE
                     LRRFKQIVTGG"
     gene            6311..6748
                     /locus_tag="VFR42_02355"
     CDS             6311..6748
                     /locus_tag="VFR42_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368023.1"
                     /translation="MPLAGLVLFTVVSYQSSRVNDELQRSPNRYYWWSSLRLDTDPLN
                     RNPKPAERCKDDPDHCASWDVSSPRVAPGWLDKVLVVSAFPAFLAAFALVLAFGRQGT
                     SEVLIFMVVMPTFLFGWYYFIGRLIERWFYKRARAKSTPLKMT"
     gene            complement(6762..8504)
                     /locus_tag="VFR42_02360"
     CDS             complement(6762..8504)
                     /locus_tag="VFR42_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0368024.1"
                     /translation="MNILGINAYHGNASAAILCDGKLVAAVEEERFNRVKYAAGFPAA
                     AIRYCLQAAGISLAQVDHVAVPRNPYARLGTKLFYALRMPSFARERAKVLAKFTGIPE
                     ALAAAFDVAPAKLKARFHRVEHHVAHLASSFYCSPFEQAALLSADGLGDFASSMWGAG
                     FGRRMNFHGSVAFPHSLGLYYSAVTQYLGFLKFGDEYKVMGLGAYGQPEFLPEFRKIV
                     LSNGRRFHLGLEYFTHHRTGPEMSWAEADKTPVLGKMYSNEMPKKLGPVRAPEAPIEQ
                     RHKNLACSLQTRLEEVYLGMLQRLQAETGMKSVCLAGGVAFNCVANGKIPASTGFENV
                     YVHPAAGDAGLAVGAAQYVWHQVLGNPRGFVMEHAYWGPDFSREEIRAAVTASHLGRN
                     GCTVSELPEGELVRQTAGIIADGKILGWFQGRAEWGPRALGNRSIVADPRRAEMKEIL
                     NRRIKHREIFRPFAPSILAEKTGEWFEQSHPSPFMTMAYAVKHEKRDKIPAPTHVDGT
                     GRLQTVTPTANPRYWRLISEFERQAGVPVVLNTSFNDNEPIVCKPQEAIDCFERTQMD
                     ALVLGDFLITRTSN"
     gene            complement(8550..>9134)
                     /locus_tag="VFR42_02365"
     CDS             complement(8550..>9134)
                     /locus_tag="VFR42_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEU0368025.1"
                     /translation="NLMEPRFRPSSVAADGYSLEPTWLGWLQRGADEARQMEAAGGVS
                     WMEMRSYMASTLLRDTDSVSMARSLEVRVPLLDTPLVEFVNALPDAARRKDGVQKALL
                     AEALGSLLPPEIAAQKKRTFTLPWEQWMRGPLKARVEVSFAHIAPELAPLLKIAGVRS
                     VWQAFLAGQASWSRPWAIFALNEWARRGGAAPAD"
BASE COUNT         1923 a         2958 c         2489 g         1764 t
ORIGIN      
        1 atcaccggcc gggaagaaaa tctcacgcat cttaagggca acggaaagtt ttcgttcgca
       61 cgagccgatg tcagcgatca gctaaatgtc gcgggcgacg tggcgtacgt cctgcacttc
      121 gcatcaccgg ccagcccgcc cgactatctg cgccatccga ttgaaaccat gcgcgtcggc
      181 tcggtgggaa cacagaacgc attggaacta gcgcgagcca aaggcgcgaa gttctttctg
      241 gcttccacca gcgagtgtta tggcgatccg gaaatctctc cgcagcatga ggaatactgg
      301 ggccacgtga actcggtggg cccgcgcagc gtatacgacg aagcgaagcg cttttccgaa
      361 gcgctgacca tgggctacca ccgtcactac ggcgtggata cgcacatcgt gcgcatcttc
      421 aatacctatg gcccgcgcat gcggctgaac gacggccgcg cgctgcctaa ttttgttttt
      481 caggcgctaa gcgagaagcc catcacggtt tatggcgacg gcaagcagac gcgcagcttt
      541 tgttacgttt cggatttgat tgacggcatc tttcgtctgg ccatgtccga ggaacacttg
      601 ccagtgaatg tcggaaatcc ggaagagatt acgctgctgg agttcgcgca gcgtgtgcgc
      661 ggtcattttc cgaaagcggg accaattgtt ttcgagccgc tgccgcaaga cgatccccgg
      721 cgccgctgcc cggacatcgg caaagccaaa cggctgctcg gttgggaacc caaggtggcg
      781 ctaagcgacg gcctcaggaa aacgattgaa tactttcagg aacaatttgc cgctgagaaa
      841 acagcgtaga agaatcgcag ctattcgcgg ccgaaggaaa cgtactcgaa ccccagcgct
      901 ttgattatcg ccggtgaata gaggttgcgg ccgtccacaa tcagttttcg cgccatcact
      961 ttgccggcct tctcccagtc cagcttcctg tactcatccc attccgtgca aaccaaggcg
     1021 gcgtgagcgc cttccaatgc ccggtacgga tcttcgtgga aatgcgcctg cgggaattgt
     1081 tttttggctt tctccagcgc ctccggatcg caggcatgca cttccgcgcc ttcctccaat
     1141 agccgccgca gcacgtccaa tgcaggcgca aaccggatgt cgtcggtgtt ggctttgaag
     1201 gccagcccga gtatcgctaa gcgtttcccc ttcaccaccc acagagcctt gctgattttg
     1261 tccagaaatc ggtcaatgcg ctgcttgttg acccgttcgg ccgctttcaa tatttcgaag
     1321 tccaccccga ccgtcgccgc caggctgata aacgcctgga tgtccttggg aaaacaaaat
     1381 ccgccaaagc ccagtccggc gcgcaagaat tgcgtcccga tgcgcgggtc aagccccatc
     1441 gcgtgagtga cctcttccac attggcgccc agtttttcgc acaagtcgga caccgcgttg
     1501 gcatagctga tcttcaacgc cagaaacgaa ttggaagcat gcttgatcag ctcagcgctg
     1561 ctgatagtgg tgaccagaaa ttctgccttc tgcatatcgg gacacttgcc ggcgtgcacc
     1621 gggcaggtaa acttacgttc cagaatcggc cggtaaattt cgcgcagctg atctgctgcc
     1681 gcttggtctt cgactccgac cacgatgcga tcggggtgaa agaaatcctc aaccgccgtg
     1741 ccttcccgca aaaattccgg attcgacgct acgcgaagac cattgccgcc acggctataa
     1801 gcagaaagga cgcgctgcag ttcgatgccg gtccgcgccg gcacagtgct cttttccacc
     1861 accagcttgg gcgaacgcgc ctccgccgca atctgctgcg ccacgtggtc gatagcggaa
     1921 agatccgcgc tgccatcggg ccgcggcgga gtacccacgc agataaagat ggcttcgccg
     1981 aagcgaatcg cttcgccggc gttggcagtg taggaaattt tcccgctatt gcccgcggaa
     2041 accagaatct tgtccaggtg gtgctcgtag atgggaacct ggccggcctt caattgcgca
     2101 atgcgcgcag cgtcgatgtc cgtgcagatc acttcatggc cggcttccgc caggcacgcg
     2161 cccgtaacca gacccacata accgcaaccg atcaccgata aacgcaaggg catctcccac
     2221 gttcgagaat taccacgcgg actgggcagc gcggcgaaag actgcctgca aaaagcgaag
     2281 ccatcgagca gttcaaaagg acgatcgcgg ccgcacgccc atcaacaagc cgcgcaaacc
     2341 gagcaaaatc agacgcggct ggcggcggag gacgttccac agcaaccggg tggaatggtc
     2401 gcggtgaatt tcgccgagcg attcccgtgt ggccgaattc aacaggtcaa ccagatagga
     2461 atagtgttcc tgctcaaagt gatgcagcgt cttgcgaatc agccagtgcg tggccagttc
     2521 gcgtcgcagc gcgcgtaact ctccgttgcc gttccctcgc aacaacgcgt cggccactcc
     2581 ctgagcgcct cggaatccgg taacaatgcc gcccaccgtg gaaactttca cttgtgccgc
     2641 ggcgtcgccc accagaaata ccttgccgtt gcctacgcgt cgctccacgg gaatccactt
     2701 gcggtaaacc gggatgcgcg cgccttgcca ccccatcggc tgcatatttt tcttttccag
     2761 aaagcgctcg atgcgccgct tggtttcctg accactgcct tcgccgatga tacccaacgc
     2821 gccgcgtgat tcgttttcgg ggatcagcca gtaaaaataa ggagtgtcgt cgggaacaaa
     2881 ccacacacgt gtggtatccg cccggcaatc cttgggcaac ttcacgatgg cttgcacaag
     2941 aggcacggtt tcgacgggcg gccaacctgc gcagcggcca acacgagtcg cggccccgtc
     3001 tgcccccacc acgtgatcgg cgtgcaattc ctcccggcga ccgccgcttt cgactttcag
     3061 gcgcaacccg cgcgagttcg ggccgaggcc cagaaagcgc gtgtcgaatg agatcttggc
     3121 gccggctttg ccggcctcac gggccagaga cggaatcaaa cgcgcccgtt caatgatcag
     3181 gtcgggcttg ttgagcgcaa cctccgcgga acgcccgtca gtgaacaatt cgaagcggcg
     3241 gatttcgttg acgatgctcg aggccgccac ttcgcgcaac tgttcacgaa aatgatgcgt
     3301 cacaatcaac gaacgaggcg ctggatccag cccgcacttg gcgtcaagca cgcgcacgtc
     3361 gcagccgccc gccgctactt ttgcggcagt cagcagcccc gctgcggaac tgcccaccac
     3421 gattacactg ccttttgtct tgtcaggctg gtcttcgtac actcgtccgg ctcatcccat
     3481 cgacccggaa gcccacgcgg ggctccgggt gctctcagca aaatgtcctt gactctccac
     3541 tcgcaggctt tcgggcatgc gctgtagtag cgctaaaacc agcgagcggc cacaaaagta
     3601 taacgaatcc actggtgcgg gaaaactcta cagcacacag attagtcggt cgcagcggcg
     3661 ggcttaggaa cctgacgttc atagggagcg cgctccagat aagcgactcc tgccagtgca
     3721 gctgcgaccg caaagacgcc cgcgacaatc gcaaaggaga taggcatgat gctcaccagc
     3781 agcgcaaggg cgccggaaac cgcgccgatc atgtacatgg tcaggaccgc tccgcgctgg
     3841 ccaagcccca tattcgccag acgatgcgcg gcgtggtcct tgccaggagt cgcgaaagga
     3901 agcaatccgc gacggctgcg cgaaatggtg accagcgtcg tgtcaaaaat cgtagcgccc
     3961 agaatgagaa tgggaaccag ccaggcggaa accagatttg gctgcgcaaa gcgcagcttc
     4021 agcgccagcg tggccatcag aaatcccaga aacattgcgc cgccatcgcc catgaaaatc
     4081 ttggcgggct tgaaattcca gcgcaaaaag ccgcaagccg cgccgagcac tgccgcagcc
     4141 agtacagtca ccagcacctg gccgttcaaa taggcgagca gcgcaaaaaa cgcagcagcc
     4201 atggcggcca ctccggcgca caagccatcc atatgatcga gaatgctgaa ggaagcggtg
     4261 ataccgacaa cccagatgat ggtcagcgcg gcgtcgagaa cgtcgcccgt tcggccaccg
     4321 actagcgtcg agaaaatctg cgcgcgaatt ccacttagca gcaaaatgac cgccgctagc
     4381 ggcatgccta cgaatagctt gatctgatgg tgcagcagtc ctcgatcatc gaggatgcca
     4441 acggcggcga tcagcgtagc cccggccaga attccccaca cttggtcacg agcggcgcct
     4501 tgcacaagaa agagcaccga caaagcaaca ccgccataga tggctaaacc gccgagcaag
     4561 ggaatcggct gcaaatgaac tttgcgcggt ccgggacggt ccaccattcc aacgcgcagc
     4621 gcgaatgcgc gaacgggcat agtgagggtt agagatgcca gtagtgcaat caaaaacgct
     4681 agcaggccag cggccatgcg ccctcagttt tcagagttac ggaaccgatg cgggccggtg
     4741 tcatcggtgg acacacgcga ctcagtgccg gattggggga tccggctccc aagtaaattg
     4801 ccaggagttg accagggacg gtcaacgagc tgctccacgc agcccataaa ccatagcgcg
     4861 gaaaatccag attggctagt taaaatacga agaaattgaa aatacagata actatgtaag
     4921 ctctaggtca agcataaaat atgcttcatg gcagaagaat tttggcttct gcttcgttcg
     4981 ccggactaac cacccgtcac gatttgttta aatcttcgta attcgttcgc ccggtcatat
     5041 gcgggagcag cctgccgggc ggcgcctgcc atttgcgcca agcgctccgg agctctcgcc
     5101 agcgcgcgaa tcacgctggc cacctcttcg ggacggtcgg gatccgcgcc cacgccgaat
     5161 ccgaactccc ctcccaaagc caccgcgtcg gtttcccacg gcgccacggc aacgatcggc
     5221 tttccggccg cgaggatccc gtacatcttg ctgggcacca ccacgccctc cagcccgcgt
     5281 ctgacggtga tcaagtgagc gtcggccgcg gccagaaccg acgggacctt gttagaagga
     5341 aaaaacggca agaatcgaat gttgcccact ccgtcggcca gcacttcgat ttccgctcgc
     5401 tgcgcgcctt cgccgacaaa tacgaaaccg ataccatcgc tggcaagttg cttcgccgcg
     5461 gtgaccaacg tggaccacgc gccgtaaaat cccaggttcc cggcgtgtag cagtacgaat
     5521 cggaagtcgc cgcgaattgt ctgaataact tcgggatcga gctgcggctg gggtgaatca
     5581 gccggtacta tctccgcgcc gtcccgcacg atctcgattc gctcccggcg aacgcccttc
     5641 gccagaatgc gcttgcgcat gtcctcgcct aacacgatta ccgttgtggc gcggcgcagc
     5701 gcccaacgat gcagcttttc ccaaacccgt gccaggacac cgggcttcac gatcgacccg
     5761 cctaccgcca tatcggggta catatcgcga atgttgtaga aatacggtct ccgtttcagc
     5821 attgccacga acgcgccgat aattccctcg aacggcgggt cggtcatcgc caggaccgcg
     5881 tcgcatggcg taaacagagc tcgcggtatt accagcgccg tgtaggtgag gtagttcagg
     5941 acgcgccgct tcatgctcag gcggggaaaa tcggtcgagc ccacgcgcac gacctgccgg
     6001 ttcgcggtcc cttctgtctg gaacagacgc cagggacgcc gctccgtcgg gtcgtaagac
     6061 ggccgcccgc acaagatcgt gacgtcattg ttcttcgcca gtacgttcac aaccgtttcc
     6121 gccattttcg cggttgccga tgtgtccggg gggtagtaga gattcagcag caggatccgc
     6181 accgatagat tgtacacggg actcttcggc cgccccgctt gccgtcatcg cgacatactg
     6241 tgaaattcgc cagtccacac cgccgccgct ggtgtttcaa taaagtatgc gcttgcggtg
     6301 gagtgttctc ttgcccctgg ccgggttggt cttgtttaca gtggttagct accagtcttc
     6361 gcgcgtcaat gatgaactgc agcgctcccc caaccgctac tactggtggt cttcgctccg
     6421 cctcgatacg gatccgctca accgcaaccc caagcccgcc gagcgctgta aagacgaccc
     6481 cgatcactgc gccagttggg acgtttcgtc cccgcgcgta gctccgggtt ggctggacaa
     6541 ggtgctggtc gtttcggcct ttcccgcttt tctggcggct tttgcgctgg tgttggcgtt
     6601 cggccgccaa ggcaccagcg aagtgctgat tttcatggtg gtcatgccca cgtttctctt
     6661 cggctggtat tactttatcg gacggcttat cgaacgctgg ttttacaaac gcgctcgcgc
     6721 aaaaagcacg ccgctaaaaa tgacctgagt ccggccaggc atcagttcga tgttcgcgta
     6781 atcaggaagt cgcccagcac cagcgcatcc atctgcgttc gttcgaaaca gtcgatggct
     6841 tcctgcggct tgcaaacaat gggttcgtta tcgttgaacg aagtgttgag gaccaccggc
     6901 acgccggctt gccgctcaaa ttcactgatc agcctccaat aacgtggatt cgccgtcggc
     6961 gtcaccgttt gcaatcgccc cgttccatcc acatgcgtcg gcgccggtat tttgtcgcgc
     7021 ttctcgtgct tcactgcgta cgccatggtc atgaacggcg aaggatgcga ctgttcgaac
     7081 cattctcccg tcttctccgc gagaatcgaa ggcgcgaacg gccgaaaaat ttcgcgatgc
     7141 ttgatgcgcc gattcaagat ttctttcatc tcagccctgc gtggatcggc cacgatgctg
     7201 cggttgccca gcgcccgcgg cccccactcc gctcgtccct ggaaccaccc gagaatcttt
     7261 ccatccgcaa tgattcccgc cgtctggcgc actaactccc cttccggcaa ttcgctgacc
     7321 gtgcagccgt ttcgccccag gtgcgacgcg gtgaccgccg cgcgaatttc ctctcgcgaa
     7381 aaatccggtc cccaatacgc gtgctccatc acaaatccgc gtggattccc cagcacctgg
     7441 tgccacacat attgcgccgc gcccaccgcc agcccggcat cgccggcggc tggatgcaca
     7501 tagacatttt caaatccggt cgaagccgga atttttccat tagccacgca attgaacgcc
     7561 acgccgcccg ccaagcacac cgatttcatg ccggtttccg cttgcagccg ctgcagcatc
     7621 cccaaataaa cttcctccag ccgcgtttgc agcgaacacg ccagattttt gtggcgttgc
     7681 tcgatcggcg cttccggcgc ccgcaccggc ccaagtttct tgggcatttc gttggaatac
     7741 attttcccca gcaccggtgt cttgtctgct tctgcccagc tcatctccgg cccggttcga
     7801 tggtgcgtga agtattccag ccccaagtga aatcgcctgc cgtttgaaag cacaatcttc
     7861 cgaaactccg gcaagaactc cggctggccg taggcgccca gtcccatcac tttgtattcg
     7921 tcgccaaatt tcaagaagcc caggtactgc gtcaccgcgc tgtagtacag ccccagcgaa
     7981 tgcggaaaag ccaccgatcc gtggaagttc atccgtcggc caaaacccgc gccccacatg
     8041 gagctagcaa aatcacccag accatccgcc gaaagcagcg ccgcctgttc gaatggcgag
     8101 cagtagaagg agctggctag gtgcgccacg tggtgttcca cccggtgaaa tctcgccttc
     8161 aatttcgccg gcgcaacatc aaaggccgcc gccaacgcct cgggaatccc ggtaaacttc
     8221 gctagtacct tggcccgttc ccgcgcaaac gacggcatcc gcagcgcgta aaacagtttg
     8281 gtccccagcc gcgcataagg attccgcggc accgccacat gatccacttg cgccaggcta
     8341 atcccggccg cctgcaaaca atagcgaatc gccgccgccg gaaatcccgc cgcgtacttc
     8401 acccggttga agcgttcttc ttccaccgcg gcgaccagct tcccgtcgca caaaatcgct
     8461 gccgaggcgt tgccgtgata cgcgttgatc ccaagtatgt tcatgtcgcg aacggctgga
     8521 ctttcagtgt aacccagttg cggaaacgat cagtccgcgg gcgccgcgcc acctcggcga
     8581 gcccattcat tcagcgcaaa aatcgcccat ggccgcgacc aagaagcttg gcccgccaga
     8641 aatgcctgcc aaacgctccg cacgcctgcg atcttcagca gcggcgccag ctcaggcgca
     8701 atgtgcgcaa agcttacttc tacgcgcgct ttcaacggcc cgcgcatcca ctgttcccag
     8761 ggaagcgtaa acgtccgctt cttctgcgcg gcaatttccg gcggcagcag tgatcccagc
     8821 gcctccgcca gcagcgcttt ctgtacgcca tctttccgcc gcgccgcgtc cggcagggca
     8881 ttcacaaact ccaccagagg cgtgtccagc agcggcacgc gcacctccag cgaacgtgcc
     8941 atgctcaccg aatccgtatc gcgcagcagg gtgctggcca tatacgaacg catctccatc
     9001 caactcaccc cgccggccgc ctccatttgc cgcgcctcat ccgctccgcg ttgcagccag
     9061 cccagccagg tcggctccag tgaatatcca tccgccgcca cgctgcttgg ccggaatcgc
     9121 ggctccatca gatt
//
LOCUS       DASTPM010000133         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_4381, whole genome shotgun sequence.
ACCESSION   DASTPM010000133 DASTPM010000000
VERSION     DASTPM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4381"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..249)
                     /locus_tag="VFR42_02370"
     CDS             complement(52..249)
                     /locus_tag="VFR42_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368026.1"
                     /translation="MPSISKKKINFSTVFAGQAIGIKEVHDDIWLVSFMDYDLGYFDL
                     ETRVLEHSKIRSVRKRYPCSR"
     gene            522..1370
                     /locus_tag="VFR42_02375"
     CDS             522..1370
                     /locus_tag="VFR42_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0368027.1"
                     /translation="MQLIRWGRPGEEKPGILTNEGLRLDVSGFGSDFDEHFFASGSLQ
                     KLQTWLAANRATAPSVPDSARLGPPICRPSKIVCIGLNFRDHAAESKMEPPKEPVLFF
                     KSTTSLVGPNDALKIPRNAEKVDWEVELAVVMVKRASYVSEADALDYVAGFALHNDYS
                     ERAFQLERGGQWVKGKSADTFAPLGPFLATRDELPNFAQLKMWLKVNGVFRQNSSTSE
                     MIFGVPFLVSYVSQFMTLLPGDVISTGTPAGVGLGMKPPQYLKPGDVVELGIAGLGQS
                     RQLVIA"
     gene            1531..>2182
                     /locus_tag="VFR42_02380"
     CDS             1531..>2182
                     /locus_tag="VFR42_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0368028.1"
                     /translation="MNPKVLQLNPHDNVLVALQNLSSGESLSFGAVSYTLGSPVSAKH
                     KFATRDLAPGDPILMYGVIVGKAVEPIAKGHALTTRNLCHEAAPFSEKSEDYRWTPPD
                     ISRWADKTFRGYHRSDGQVGTRNYWLVVPLVFCENRNIGVLKQAFEEELGFAAPQIYR
                     RQVAELARLYKEGKSSEVSSAALSNTTAAPARSKIFENVDGIRFLLHEGGCGGTRED"
BASE COUNT          488 a          686 c          537 g          471 t
ORIGIN      
        1 ccgagcgcgc cattcctaga cggtccggcc cggacacatg ggttacacaa tctaccggct
       61 acatgggtaa cgctttcgga ccgaacggat tttcgagtgt tcgagcaccc gagtctccag
      121 atcgaagtat cccagatcat aatccataaa gctcaccagc cagatgtcgt cgtgcacttc
      181 tttgatgccg atggcttgtc ccgcaaagac cgtgctgaaa ttgatttttt tcttgctaat
      241 cgacggcaca ctgcttcagt ggagcatcag atgaccgaag tgttcagaaa cgaatcgcac
      301 ttacttaaca agtctgctaa gtaaataagc tccgacgttc gaggatcaga gtcacgcttg
      361 agttgtcaag ccaacctaga cccccatctg tagcagaact gatacgaaat ccgaaagaaa
      421 agttctaaga tcgtcgcctt gggcgcgcca ttttcacgac cacgcctccg ctttcttggc
      481 cttcactgat acaatctccg gttcacagga gcgcatcgcg catgcagcta attcgatggg
      541 gtcggcctgg cgaggagaaa ccggggattc tgacaaacga gggcctccgg ctcgacgttt
      601 ccggttttgg ttccgacttc gacgaacatt tctttgcgtc aggcagcttg caaaaattgc
      661 aaacctggct ggcagccaac agggcaactg cgccgagcgt tccagactcc gcgcgcctgg
      721 gcccgcccat ttgccgtccc agcaagatcg tctgcatcgg cctgaacttc cgcgatcacg
      781 ccgccgagag caaaatggag ccgcccaagg agccagtcct tttcttcaag tcaactacat
      841 cgttggttgg cccaaatgac gctctgaaaa ttccgcgcaa tgcagaaaaa gtcgattggg
      901 aagtcgagct ggccgtggtc atggtgaaga gagcctctta cgtttcagag gcggacgcac
      961 tcgactacgt cgctggcttc gctctgcata acgattactc cgaacgtgcc tttcaactcg
     1021 agcgcggcgg ccaatgggtc aaaggcaaaa gcgccgacac tttcgcgccg ctcggcccct
     1081 tcctcgccac ccgcgatgaa cttcccaatt ttgctcagct caaaatgtgg ctcaaggtca
     1141 acggcgtctt ccgccagaac agctccactt ctgaaatgat ttttggtgtg ccgttcctcg
     1201 tctcctacgt cagccagttc atgacgctgt tgccaggcga tgtcatcagc accggcacgc
     1261 ccgccggcgt tggcctcggc atgaaaccgc cgcagtatct caagcccggc gacgtcgtcg
     1321 aactcggcat cgcaggttta ggccaatccc gccagctagt catcgcttga atgcgcgcaa
     1381 acgaacccga atcctctctt tgaggtagga accttagctc cgacagaaat gcttggcact
     1441 cactcgggtt ttagcccctg aggaattgct gtcatcgcgc cgtcaaaatg ttcgccactg
     1501 taatacaatt gcacttctcg acgaattcaa atgaatccca aggtcctcca actaaacccg
     1561 cacgacaacg ttctcgtcgc cctccagaat ctttcttcgg gcgaatcgct ctccttcggt
     1621 gccgtgtcct acacgctggg ttcgccagtt tcggccaagc acaaatttgc cacccgcgat
     1681 cttgccccgg gcgatcctat cctcatgtac ggcgtgatcg tcggcaaagc ggttgagcca
     1741 atcgccaaag gtcacgcgct caccacgcgc aacctgtgcc acgaagccgc gcccttcagt
     1801 gaaaaatccg aagactatcg ctggacgccg cccgacattt cccgctgggc cgacaaaacc
     1861 tttcgcggct accatcgctc cgatggccaa gtaggaacgc gtaactactg gctggtcgtt
     1921 ccgctagttt tctgcgaaaa ccgcaacatc ggtgtcctca aacaagcctt tgaagaagaa
     1981 ctcggcttcg ccgctccaca aatctatcgc cgccaggttg ccgaactcgc gcgcctatat
     2041 aaggaaggga agtcctccga agtctcctca gccgccctct cgaacacgac cgccgctccc
     2101 gcccgctcca aaattttcga aaacgtcgac ggcattcggt tcctcttgca cgaaggcggc
     2161 tgcggcggca ctcgcgaaga ct
//
LOCUS       DASTPM010000134         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_263962, whole genome shotgun sequence.
ACCESSION   DASTPM010000134 DASTPM010000000
VERSION     DASTPM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263962"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFR42_02385"
     CDS             <1..858
                     /locus_tag="VFR42_02385"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEU0368029.1"
                     /translation="YGSPLHLVHFYFALGVIWYVGFIDDLLNLPPEVRLLAQLAAGFL
                     LSQTRWNLTLFNHPILDSILTCLFVAVFVNAFNFLDGSDGIAGGVAALVALGYAAVYA
                     TPTTSAGGAVAWSLLGACLGFLAYNFPPARIFMGDSGSTMLGFLIAFLSLDFYRVHHR
                     IGSHWLLPLVFAALPLMDFFLAVIRRLRNRVSPFSGDRGHFYDLLLDRGWSSLHVALG
                     AYAVTGALLVVGWLCIHPDWKISVLLLFVLAFLFAVSLRNFEGLRQKSQLFFGSPQRP
                     SNDGTSSDD"
     gene            912..1685
                     /locus_tag="VFR42_02390"
     CDS             912..1685
                     /locus_tag="VFR42_02390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0368030.1"
                     /translation="MALWKRANAAIRSYCSLNRNREFTLLRKRAEESTRGALLDIGSG
                     DGFWTERFAQRFASSYGLEPDPQALRTAQKLHGERTKYVHGFAENLCFEDEAFDCVVS
                     VSCFEHFRSAQAALQESFRVLKPGGKLAISVDSLLQDNSSKEFRAWHSKKYFVTEYFS
                     EKRLTELLVNAGFIVEHRHTRHLINSRFSARLRELYLHHPRSLLLFFPLLYLLVLAGD
                     RWGKQVPGQIVVMSARKPGRYAGEPESSFRDLDPALSPA"
     gene            1720..>2123
                     /locus_tag="VFR42_02395"
     CDS             1720..>2123
                     /locus_tag="VFR42_02395"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU0368031.1"
                     /translation="MILWNDQRYTPALRFGIAGDFLPAAGLAPAPGESWTECAELVRP
                     LFQDLAFSVANLECPVGVPGIPPKVKTSLGATFAAGVAALDYLGALHASVVGIANNHL
                     YDYGKAGAERTLQNVIARGKFSACGFGRSLADA"
BASE COUNT          378 a          624 c          584 g          537 t
ORIGIN      
        1 tacgggtcgc cattgcatct ggtgcacttc tactttgcgc tcggcgtgat ttggtatgtc
       61 ggcttcatcg atgacctgct gaatctgccg cccgaggtcc gcctgctcgc gcaattggct
      121 gcgggattcc tcctcagtca gacgcgctgg aatctcacac ttttcaatca tccaattctc
      181 gattccattt tgacttgcct gttcgtcgcg gttttcgtga acgcattcaa ctttctcgat
      241 ggttcagacg gaatagcggg cggtgtcgcc gcgctggtag ctttgggcta cgccgcagtt
      301 tacgctacgc ccacgaccag tgccggcggt gcggtggcgt ggagtttgct gggcgcctgc
      361 ctgggttttc tcgcctacaa ctttccgccg gctcgcatct tcatgggcga ttcgggcagc
      421 accatgctcg gtttcctgat tgcctttctt agtctggatt tttaccgcgt tcatcatcgc
      481 atcgggtcgc actggctcct gcctctggtg tttgcggcgc ttccgttgat ggattttttc
      541 ctcgcggtga tccgccgttt gcgcaatcgg gtttctccct tttcgggcga ccgggggcat
      601 ttttacgatc tgctgctcga tcgaggttgg tcgtcgctcc acgtcgctct gggagcatat
      661 gccgttacgg gcgcgctttt agttgttggt tggctctgca ttcatcccga ctggaaaatc
      721 tctgttttgc tgctttttgt gttggccttt cttttcgccg tgagcttgcg caatttcgag
      781 ggtctgcggc aaaagtcgca gctcttcttc ggttcgccgc agcgcccaag caatgacggt
      841 accagttccg acgattgacg ctcccaggat tttcccggcc aaacgcattc atcaacaaat
      901 acgaggacca catggctctt tggaaacgcg cgaatgccgc aattcgcagc tattgctcgc
      961 tgaaccgcaa ccgcgaattt accctattgc gcaaacgcgc cgaagaatcc actcgcggcg
     1021 cactgctgga tatcggcagc ggcgacggat tttggacaga acgctttgcg caacgttttg
     1081 cgtcatcgta cgggttggaa cccgatccgc aagcgctgcg cacggcgcag aaactacacg
     1141 gtgagcgaac caaatacgtg cacggcttcg cggaaaacct ttgctttgag gatgaagcgt
     1201 tcgactgtgt ggtcagcgta agctgcttcg agcatttccg cagcgcgcaa gccgctctgc
     1261 aagaaagctt tcgcgtcttg aaacctggcg gaaaactcgc catctccgtg gacagtctat
     1321 tgcaggacaa ttcctcgaaa gaatttcgcg cctggcacag caaaaagtat ttcgttacag
     1381 aatacttcag tgaaaaacgc ctgacggaac tgctggtgaa cgccggattt atcgtcgaac
     1441 atcgccacac gcggcatctg atcaactcgc gattcagcgc gcggctgcgc gaattatact
     1501 tgcatcatcc gcgttcgctc ctgctgtttt ttccgctcct gtatttgctg gtgctggcgg
     1561 gcgatcgctg gggaaaacag gtccccggac aaattgtggt gatgagcgcg cgcaaacccg
     1621 gccgctacgc cggcgaacca gaatcgtctt ttcgcgatct ggatccggcg ctttcaccgg
     1681 cctgacaaag taaagccaag cgatgcattt tcggacatca tgattctctg gaacgatcaa
     1741 cgctatactc cggcgctgcg tttcgggata gccggcgact ttctccccgc cgccgggctc
     1801 gctcccgctc ctggagaaag ctggacggag tgcgccgagt tggtccgccc gttatttcag
     1861 gatctggcgt tcagcgttgc caacctcgaa tgcccggtcg gcgtccccgg aattccaccc
     1921 aaagtaaaaa cttctctcgg agcgaccttt gccgcgggcg tcgctgcgct cgactatctc
     1981 ggcgcgttgc acgcttcggt cgtcgggatc gccaacaatc acctctacga ttacggcaaa
     2041 gccggcgcgg aacgtactct gcaaaatgta attgcgcgcg ggaagttctc tgcgtgcggc
     2101 tttggccgtt ctctcgctga tgc
//
LOCUS       DASTPM010000135         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_436860, whole genome shotgun sequence.
ACCESSION   DASTPM010000135 DASTPM010000000
VERSION     DASTPM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436860"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VFR42_02400"
     CDS             <1..992
                     /locus_tag="VFR42_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0368032.1"
                     /translation="REGGREGLLEYLEPAWVKSAPTFAPNVAAELDPVFSSSSSAVPD
                     VASPITNIDRTVKLYIGGKQARPDSGYSVPVYGLKGELLGEAPLGNRKDIRNAVEAAR
                     KAEGWAKTTAHNRAQILFYIAENLSQRADEITKRLSAAIGEKQAAQEVQASIERLFSY
                     AAWCDKFDGAVHNPPFRNISIAMNEPVGTVGVICPAESPLVGFFSLALPLICAGNTVV
                     AVPSEKYPLITGDLYQVFETSDLPGGVINLVTGRSSELLKVLAEHDDLDAIWCYGEES
                     LCSAAKALSAGNLKQVWTNEGRKINWFDAVQGEGRWFLQHAYQVKNIWVPYGE"
     gene            1112..2392
                     /locus_tag="VFR42_02405"
     CDS             1112..2392
                     /locus_tag="VFR42_02405"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368033.1"
                     /translation="MNDGATETVEAKLDARAAFRYPAFVNYQFARFCIVVATEMQAVA
                     VGWQVYEITKRPLDLGLVGLAQFLPSILLFLISGHVADRFNRRNLLLLCDFGFAICFA
                     LLIAITLGGNVSTISIYVVLVLLGVVRSFNGPVSRAIFPQLVPPEVFASSVAWGSTFW
                     QAATILGPVVGGMLYAFSERVFGHGAGPSAVYACSLLAAAIAVVLMLRVPSQEKERNK
                     PSATLHTVFEGFRYIWREKMILGAISLDLFAVLLGGAVALLPVYAREILQTGPWGLGI
                     LRSAPGVGAGIMAIAIAHNPLKKKAGATMLWCVAGFGLCTVLFGVSRSFAISLVALVL
                     VGATDMVSVIVRATLIQVATPDEMRGRVNAVDMIFIGASNELGQFESGITAQWFGTVP
                     AVILGGIGAIVVTGLWAWIFPQLRKVDQLPLKEL"
     gene            complement(2418..>2647)
                     /locus_tag="VFR42_02410"
     CDS             complement(2418..>2647)
                     /locus_tag="VFR42_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEU0368034.1"
                     /translation="ALRVLAYGGVYLAGGIALKILPLLQKSTFCTAFADKGQLAPLLA
                     NIPIAVVLNEDAPLLGAAHSALTVLQGGKLT"
BASE COUNT          529 a          784 c          760 g          574 t
ORIGIN      
        1 gtcgcgaagg aggccgcgaa ggcctgctcg aatatctgga acccgcttgg gtcaaaagcg
       61 ctcccacctt cgcgccaaat gtagcggcgg aacttgaccc ggtattttcg tcttcctctt
      121 cggctgtgcc cgacgtagcc agtcccatca ccaatatcga ccgcaccgta aaactctaca
      181 tcggtggcaa acaagcccgc ccagattcag gctacagcgt tccggtctac ggattgaagg
      241 gcgaactcct cggcgaggct cctctcggta atcgcaagga cattcgcaac gcggtcgaag
      301 ccgcccgcaa agccgaaggc tgggccaaaa ccaccgcgca taaccgcgcc caaatactct
      361 tctacattgc cgaaaatctt tcccaacggg ccgacgaaat caccaaacgc ctctccgcgg
      421 ccatcggcga aaagcaagcc gcgcaagaag ttcaagccag catcgaacgc ctgttctctt
      481 acgccgcctg gtgcgacaaa ttcgacggcg ccgttcacaa tccgcccttt cgcaatattt
      541 cgatcgccat gaacgaacct gttggcacgg tcggcgtcat ttgcccggcc gaatcgccgc
      601 tcgttggatt cttctcgctc gctctgcccc tgatttgcgc cggaaacacg gtggtagccg
      661 tcccgtcgga gaaatatcca ctcattaccg gcgatttgta tcaggttttt gaaaccagcg
      721 acttgcctgg cggcgtcata aatctcgtaa ccggcaggtc gtccgagttg ctgaaagtcc
      781 tcgccgaaca cgacgacctc gacgccatct ggtgctacgg cgaggaatcg ctttgttcag
      841 cagcgaaagc attatcggcg ggaaatctca agcaggtgtg gaccaacgaa ggccgcaaga
      901 tcaattggtt cgacgccgtg caaggcgaag ggcgctggtt tttgcaacac gcctatcagg
      961 tcaaaaatat ctgggtgccc tacggcgaat aaatgtcgcc aatttcttta ggaggcgacg
     1021 gctttagccg tcgcgtacgg cgcggacaac cgagcggctt tagccgctcc aggggaaatc
     1081 aactgaccca gcacaaacga gcgtcggccc ggtgaacgac ggagctaccg aaacagtcga
     1141 agcaaaattg gatgcccgcg cggcgttccg ctatcccgcg ttcgtcaatt atcagttcgc
     1201 ccgcttctgc atcgtcgtcg ccaccgaaat gcaggccgtt gcggtcggct ggcaagtcta
     1261 cgaaattacc aaacgccccc tcgacctggg tctggtcggc ctagcgcaat tcctccccag
     1321 catcctcctc ttccttattt ccgggcatgt ggcggatcgc ttcaaccgca gaaatttatt
     1381 gctactctgc gactttggct ttgcgatttg tttcgcgctg ttaatcgcga tcacgctcgg
     1441 cggcaacgtt tccaccatct ccatctatgt cgtgctggtg ttgctcgggg tggtgcgctc
     1501 cttcaacggc ccggtgagcc gcgctatctt cccgcaactt gtcccgccgg aagtgttcgc
     1561 cagttcggtg gcgtggggat cgaccttctg gcaggccgcc acgattcttg gcccggttgt
     1621 cgggggaatg ctttatgcat tttccgaacg tgtgtttggt cacggagcag gaccgtctgc
     1681 cgtgtatgcg tgttcgctcc tggccgccgc gatcgcggtt gtgctgatgc tgcgcgtgcc
     1741 ttcgcaggaa aaagaacgca acaaaccgtc agcgacgctg catacagttt ttgaaggttt
     1801 ccgctacatc tggcgcgaga agatgattct cggcgcgatt tcactggatc tgttcgcggt
     1861 tttgctgggt ggcgcagtgg cactgcttcc cgtgtatgcg cgcgaaattc tgcaaaccgg
     1921 tccatgggga ctgggaattt tgcgtagcgc ccccggtgtc ggagctggaa tcatggcgat
     1981 cgccattgcg cacaacccgc tcaagaaaaa agccggcgcc acaatgctgt ggtgcgtggc
     2041 cggatttggg ctgtgcacgg tgttgttcgg cgtgtcgcgc agtttcgcaa tttcgctggt
     2101 cgcgttggtc ttggttggcg cgacggacat ggtcagcgtg attgtgcgtg ccacactcat
     2161 acaagttgca acaccggatg aaatgagggg gcgcgtcaac gcagtagaca tgatcttcat
     2221 cggcgcttcc aacgaactcg gccagtttga atcaggaatt accgcgcaat ggttcggcac
     2281 ggtgcctgcc gtgatcctcg ggggcatcgg cgccatcgtc gtcacgggat tgtgggcatg
     2341 gatcttcccg caactgcgaa aagtagatca gctcccgctg aaggagctgt aggactctgt
     2401 ctttcgtgtg cagtgagcta ggtgagtttg ccgccctgca atacggtaag tgcggaatgg
     2461 gcggcgccca atagcggagc gtcttcgttc agcacaaccg cgatgggaat gttggccaat
     2521 agcggcgcca gctggccctt gtcggcgaac gccgtacaga acgtgctctt ctgcagcagc
     2581 ggaagaatct tcagcgcgat gccgcctgca aggtagacgc caccgtaggc aagcacccgc
     2641 aaggcca
//
LOCUS       DASTPM010000136         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_4455, whole genome shotgun sequence.
ACCESSION   DASTPM010000136 DASTPM010000000
VERSION     DASTPM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4455"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFR42_02415"
     CDS             <1..212
                     /locus_tag="VFR42_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368035.1"
                     /translation="ASANAARTKSKTTPEDCARVMLHAIETPRPKPRYGVTSLSPFVK
                     WSKRLLTDSAIDAMLRRRYGVTRES"
     gene            complement(242..658)
                     /locus_tag="VFR42_02420"
     CDS             complement(242..658)
                     /locus_tag="VFR42_02420"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEU0368036.1"
                     /translation="MKFAGRRIAKVLAGIVLFASGVAAGNWHASVVAAQNRFGQPKSV
                     LHFVVYKWKNTTSQNDKDQSLAGIKTMAAKIPGIKNIWLKTERNQIRDFDGVYAIEFT
                     SAEAAADYAESPLHETWRKKWEELRENSLSFQVSNP"
     gene            complement(803..2551)
                     /locus_tag="VFR42_02425"
     CDS             complement(803..2551)
                     /locus_tag="VFR42_02425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU0368037.1"
                     /translation="MRKLATTILVLVLSGMGGKAVVAAPRPADQPRTMDEVVDRVVVN
                     ENHLNQEIRKFSPLVETYIQNLRPDKELGYSPAGDKYFLGRADFSKGVNLVSLTDADT
                     KGKKIFSGIGNFFSFAMQYLPDGFLQMIFIDTNGFDKQHYKFDYVRREFLGEVRCLVF
                     DVSPQPKAGKGRFLGRIWVEDQDFHIVRFNGAYGGNGHTSWYFHFDSWRTNAQPGMWL
                     PSFVYSEEKDLHYALTKKLDFKAQTRLWGYNVGHAAEEQELSKILVETPVQDDSKTSN
                     DLTPVQAQRSWDRQAEDNVSDRLQRIGLMAPRGEVDKVLDTVVNNLEVTNNLDVEPEV
                     KCRVLMTTTLESFTLGHTIVLSRGIIDVLPDEASLATIIAHELGHVVLGHRMDTTYAF
                     FDQLLVDDKDTFRHFGFARTPEEEQAASTKAVELLNNSPYKNQLANSALFLAALQARQ
                     KEIPNLISAHLGDRVMTIADIKNKVAPVAAAADATDPKDPKKNSQAIAALPIGGRVKL
                     DVWNDKLDLIKSKPVGTVAEREKMPFEVTPFMPYLTRYNIEASKPANASSTEPKSPES
                     ASIPATDPKPADPTKP"
     gene            complement(2573..3709)
                     /locus_tag="VFR42_02430"
     CDS             complement(2573..3709)
                     /locus_tag="VFR42_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0368038.1"
                     /translation="MSTMRLGVGFDTRARARSEAWKTSIGNKKVPARFDLLPDGDKRW
                     DRIGVSAIVQILVVTFFVVMPIFFPERIASIKYSVVPLLNYVTEIPVAPKPPAPPKVR
                     AKAIEPKPIEEPKLSPKQPHIFVQQKAFQPQVRQLDVKAPELVKENVFEAKLNTPING
                     PKRPRDEVKVNNLNSGSAAPATVVAPVEKVQTGGFGDPNGLPGKGNPNKTTNINRLGS
                     PALPGGDGYGNGTGGAKGIRGTVASTGFGNGVANPPQGGGGKHGVVQSGGFADASVAD
                     NAPKKKAAASDSPTTMVDILDKPRPQYTAEGRSLRIEGDVVLDLVFQADGTVQVNKVV
                     SGLGHGLDEAAVRSAQQMKFKPAKRDGQPVDFPARVRIEFRLAY"
BASE COUNT          767 a         1200 c         1119 g          901 t
ORIGIN      
        1 tcgccagcgc caacgccgcg cgcacaaaat ccaagaccac gccggaggat tgcgcgcgcg
       61 tgatgctgca cgccatcgag acgccgcggc caaagccgcg ctacggcgtc acctcgctat
      121 cgccatttgt gaaatggagc aagcgtctgc tgaccgacag cgcgatagat gcaatgctgc
      181 gccggcgcta cggggtcacg cgcgaaagct gactcgtcgg acccagagtc agcgcacaac
      241 gtcaagggtt ggaaacctga aacgaaaggc tgttttcccg cagctcttcc cacttcttgc
      301 gccaggtttc atgcagcgga ctttccgcat agtcggcggc ggcctccgcg ctggtaaatt
      361 cgatggcata aacgccgtca aaatcgcgga tctggtttcg ctctgtcttc aaccagatat
      421 tcttgattcc ggggattttc gcggccatcg tcttgattcc cgccagcgac tggtctttat
      481 cgttttgcga agtggtgttc ttccatttgt aaaccacaaa gtgcaggaca gatttgggct
      541 ggccaaacct gttttgcgca gccaccacgc tggcgtgcca attccctgcc gccactccgc
      601 ttgcaaacag aacaatgcct gccagaacct tcgcaattcg ccgccccgca aatttcatgt
      661 cgtcctccct catcccacca aagcagtgta agtacacccg cgaatagacg caacgaatcg
      721 ccaaagggct ggccggcgag caccaaaaag aaaacccggt gagctgtggc tcaccgggcg
      781 aaaaactcag ttggaattaa tttcagggtt tcgtcggatc ggccggcttt gggtcggttg
      841 ctggaatcga agcggattcc ggcgatttcg gttcggtcga gctggcattc gccggcttgg
      901 aggcctcgat gttgtagcgc gtgagatagg gcataaacgg agtcacttca aacggcatct
      961 tttcccgctc cgccactgtt cccaccggct tgctcttgat caggtccagc ttgtcgttcc
     1021 agacgtccag cttgacgcga ccgccgatag gcagcgcggc aatcgcttgc gaattcttct
     1081 tcgggtcctt cggatcggtt gcatccgcgg cggcggccac gggagcaacc ttgttcttga
     1141 tgtccgcgat ggtcatcacc cggtcgccca agtgggcgct gatcaggttg gggatttcct
     1201 tctggcgcgc ctgcagcgcc gcaaggaaga gcgcggaatt cgccagctgg tttttgtacg
     1261 gcgaattgtt cagcagttcg accgctttag tgctggccgc ctgttcttct tccggcgtgc
     1321 gcgcaaaacc aaaatggcgg aacgtgtctt tgtcgtcgac cagcaactgg tcaaagaaag
     1381 cgtaagtggt gtccatgcga tggccgagca ccacgtgccc gagctcgtgc gcgataatcg
     1441 tggccaggct ggcttcatcg ggcagcacgt cgataattcc gcggctcagc acgatcgtgt
     1501 ggcccagggt aaaggattcg agcgtcgtgg tcatcagcac ccggcacttt acttccggct
     1561 ccacgtccag gttattggtg acctccagat tgttgaccac cgtgtcgaga actttgtcca
     1621 cttctccacg cggcgccatc aacccgatgc gctgcaagcg atcagaaacg ttatcttccg
     1681 cctggcggtc ccaggaacgc tgcgcctgca ctggagtcag atcattcgag gtcttggagt
     1741 cgtcctgcac cggagtttcc accaggatct tgctcagttc ctgttcttcc gcggcgtgcc
     1801 cgacgttata gccccacaag cgggtctgcg ccttgaagtc cagcttcttg gttagcgcgt
     1861 agtgcagatc cttctcttcg ctataaacga aagaaggcag ccacattccg ggctgcgcgt
     1921 tggtgcgcca gctatcgaag tggaagtacc agctggtgtg cccattgccg ccgtacgcgc
     1981 cattgaagcg cacgatatgg aaatcctgat cctccaccca gatgcggccc aggaagcggc
     2041 ctttgccggc cttgggctgc gggctgacgt cgaacaccag gcagcgcact tcgcccagga
     2101 attcgcggcg aacataatca aacttgtagt gctgcttgtc gaagccgttg gtgtcgatga
     2161 agatcatctg cagaaagccg tccggcagat actgcatggc aaaggaaaag aaattgccga
     2221 tgccggagaa aatcttcttg cccttggtgt cggcgtcggt cagggaaacc aggttcacgc
     2281 ccttggaaaa gtccgcgcgg cccaggaagt atttatcgcc cgctggactg tagcccagtt
     2341 ccttgtccgg cctgagattt tgaatgtagg tttctaccag tggtgaaaat ttacggatct
     2401 cttggttcag atggttctca ttcaccacca cgcgatccac aacttcgtcc atggtccgtg
     2461 gctggtcggc tggacggggc gccgcgacca cagctttgcc ccccatcccg ctcaaaacga
     2521 gcaccaggat agttgttgct agcttgcgca tttctctgct cccctcagta cctcagtagg
     2581 ccagtcggaa ttctatgcgc acgcgagcag gaaagtcgac cggctggcca tctcgcttgg
     2641 caggtttgaa tttcatttgc tgcgcggacc gcaccgcggc ctcatcgaga ccatggccga
     2701 gcccgctgac cactttgttt acctggaccg tgccgtcagc ctggaatacc aaatcgagta
     2761 ctacgtctcc ttcaattcta agactacgcc cttctgcggt gtattggggg cgcggtttgt
     2821 ccaggatgtc aaccatggtg gttggcgaat cgctggccgc cgctttcttc ttcggcgcgt
     2881 tatccgcgac gctggcatcg gcgaagccgc cgctctgcac cactccatgc ttgccaccgc
     2941 cgccttgcgg cggattggcc acgccattgc cgaatccggt actcgccacg gtgccacgga
     3001 ttcccttcgc tccgccggta ccgtttccgt acccatcccc gcctggaagt gccggcgagc
     3061 ccagccgatt aatgttcgtg gtcttgttag gatttccctt gcctggcaat ccgttggggt
     3121 cgccaaatcc tccggtctgt actttctcca ccggagcaac caccgtggcc ggagccgcgc
     3181 tgccgctgtt caggttattg accttcactt catcgcgcgg gcgcttgggt ccattgatcg
     3241 gcgtgttcag cttggcctca aaaacgtttt cctttaccaa ctccggcgcc ttcacgtcca
     3301 gttgccgcac ctgcggttga aacgccttct gctgcacaaa gatgtgcggc tgcttgggac
     3361 tcagcttcgg ttcctcaatt ggtttcggtt caatggcctt ggctcgcact ttcggcgggg
     3421 ctggcggctt gggcgcaacc ggaatttcgg taacgtaatt cagcagcggc acaacggagt
     3481 atttgatgga ggcgatgcgc tccggaaaaa agatgggcat gactacgaaa aatgtgacca
     3541 ccagaatctg cacgatcgcg ctcacgccga tgcgatccca gcgcttgtcg ccatccggca
     3601 acaaatcaaa gcgggcgggc accttcttgt ttccaatcga ggtcttccac gcctcgctgc
     3661 gggcgcgcgc gcgcgtatcg aatccgacgc ctaaacgcat ggtactcatg ggtgggtctg
     3721 cgatcattaa tgtcatccgc gttctgcctt agagtagatt ccctcttgga aacatgggtt
     3781 ttccaggcga gcctggcctt cccaagttca ttggcaggaa cagaaagtgc tcttcagcgc
     3841 gaaccagagt actaatgggg ctggggagcc cgaataggac catcgtacta tcgccgcgaa
     3901 gtcgccaatg gctccctcgc gatggaaaaa tcgctggcac ctctttgaaa taacccggcc
     3961 aggcatctta gagttgcggg gttgggg
//
LOCUS       DASTPM010000137         9102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_494687, whole genome shotgun sequence.
ACCESSION   DASTPM010000137 DASTPM010000000
VERSION     DASTPM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9102
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494687"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VFR42_02435"
     CDS             complement(<1..1955)
                     /locus_tag="VFR42_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0368039.1"
                     /translation="MSTTPNSPAAAAGRGNGIPRVIVATTALLTFISFWRAAAIVLND
                     LASSAYYAGGEAEGYIGKTAPWFILAIMLFSYAVRAVYVESCSMFVRGGVYRAVKQSL
                     GGTLAKFSVSALMFDYILTGPISGVSAGQYLGGLLSELLPYAHLTTGPLSPRTIDVIA
                     ASFAIIITIYFWWENTKGIPESSEKALKIMKLVTVMVVLLIGWCVYTIILRRPSLPPF
                     PYPSNLHLTSASLGWLAHSSLPHMFGIVAIFIALGHSVLAMSGEESLAQVYREIESPK
                     LVNLKKAGLVIFAYSMIFTSLVSFFAVMIIPDDVRQGFLENLIGGLAMSLEGPFLLRL
                     IFHAFVVVVGAVILAGAVNTAIVGSNGVLNRVSEDGVLSDWFRQPHPRFGTSYRIINI
                     IVVLQILTILISHGDVTFLANLYAFGVIWSFAMNGLAVFVLRYTNPGQREFQVPLNFN
                     IGKTQFPLGLGLITLTLFLIAFVNLFTKPVATMAGGVFSVILYVVFTISEKRARHTGV
                     AHVEMDQFNLEIEGELTPEAVGAKPGNILVPVSNHFNMYHLGNVLDRVKPGRRDVVVL
                     HVRILRRTPSGEQEFEADQLFGSIEQHLFSQALSMAEKRGKSIRLAVVAANDLWDGIL
                     RAGHSLQSSTIVVGTSGKWQSEEQARQ"
     gene            complement(2046..2378)
                     /locus_tag="VFR42_02440"
     CDS             complement(2046..2378)
                     /locus_tag="VFR42_02440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0368040.1"
                     /translation="MSVERRRAARIPFTASAEIIDDEENTRTSSQVSDLSLHGCCVKA
                     PNPFPVGTSVTIEIYRDADFLEAPATVAYFTPQKAMGLAFTSIEPRFAAVLKKWLATT
                     SVPKTLTH"
     gene            2520..2657
                     /locus_tag="VFR42_02445"
     CDS             2520..2657
                     /locus_tag="VFR42_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368041.1"
                     /translation="MNVGDPVFDHVEFAGETLNFGLGAAVHVVVEFAAEAVFRVLAIL
                     A"
     gene            complement(2780..3157)
                     /locus_tag="VFR42_02450"
     CDS             complement(2780..3157)
                     /locus_tag="VFR42_02450"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEU0368042.1"
                     /translation="MSTPIDQRRKPASVKIHVSSGAGVDIGWADGHASHFDFVYLRDH
                     CPCATCNDERVKKDSMAESSLGFASSPALPMFKPKPKAQSAQAVGQYAIQISFTDGHS
                     TGIYSYDLLRSICPCSECAAKRT"
     gene            complement(3209..4465)
                     /locus_tag="VFR42_02455"
     CDS             complement(3209..4465)
                     /locus_tag="VFR42_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0368043.1"
                     /translation="MSAHTTVPENVAAPRQASQVAIDTLVKQYGALRALDGVSFNISA
                     GEWVALMGPSGSGKTTLINILGGLDTLTSGRVVVDGVDLSRLGENQLVRYRAEKIGFV
                     FQQFHLVPYLNAVENVMLAQYFHSTTDEAEAAATLKRVGLGQRLTHLPAQLSGGEQQR
                     VAIARALINHPKLILADEPTGNLDEANEQIVINLFRELHKSGHTILMVTHDPDIARQA
                     DRRIELAHGHLSFDTAQHGPNHPMACPLANTADCCTPVSADDDIRVDHLLEQIWIFGE
                     EGQPAQAELLRVEGPAGQLPIVGTDLTPSRLLARMAESRLVELNGKRNGNFDAQHAGE
                     VRLTESGMRRARDVVRRHRLAERLFTDTFAIEDAEAHQQACRFEHIITPELDQRICSF
                     LGHPKTCPHGNPIPPGPCCEKAEAGK"
     gene            complement(4462..5703)
                     /locus_tag="VFR42_02460"
     CDS             complement(4462..5703)
                     /locus_tag="VFR42_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368044.1"
                     /translation="MMGIERAANIDQRTVPDRRSVNRSMFWRVLRRSVFANRGRLSVI
                     LLALGAGSAVTAALLNLQIDAKRRVAAEFRAFGANVTIAPRAAEVSGTMPASISDDIP
                     SATAAGPISRVALLSAVADVTPIDCDREKRKVSEAGPTVKVVVNGQTTGDRLFTDVFP
                     SRRVFQAERVYLDVNDCQLGARVAQRLGVRDNRQMRLKIGNSFDYCFAESIQDFGTAL
                     DDQAFAPLDVIQRLTGQVGQISLIELRVPGTPAQVDQTVDLLRQKLPRVDVRPIRQFT
                     EGEARLYGKISGILTATVAVVLVLTSLCVMAAMTNIAAERKNDLGLMKAIGGSVRRIL
                     RIFLAEAALLGLGAGLIGAAAGIVLSIGLGKAVFGVAAQPRLIVYPVSVALTVIVAIL
                     ASYPLRRLVHIRPASVFRGEE"
     gene            complement(5700..7016)
                     /locus_tag="VFR42_02465"
     CDS             complement(5700..7016)
                     /locus_tag="VFR42_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368045.1"
                     /translation="MFLRLIADNFARRPRRKMLTAAALSLGMAIATATLSVSLDVGDR
                     LAKEFRSLGANLVVTPEADSLPLEIGGVDYRPVNAGAYLPESDLPKIKSVFWHNNIIA
                     FAPILEVHAAASLFAAKDGQHESGDWTRDVSVIGTWSDHPVPLKDGGEFRTGIARTNP
                     WWRVKGRWFSDSRKECVIGAEFAAKYHLKVGEEVSLATGRVTKFPSPFTVTGIVSSGG
                     PEDEAILVPLTQAQSLAHAPGQYRKLYVSALTKPEDDFARRDPKSMNPDELERWSCSP
                     YVSSIAYSIKQVLPGSDVRVIRRVADGEGKILTRVRILLWLVTFASLLAAALAVGASS
                     AASVIERRGEIGLMKALGAASATVGWLLAAEQLLLALVGGCIGYGIGLLLARFLGTRI
                     FGVPPAPSFLVLGMVLVLAAGVTLLGSAWPLRRAANYDPAPILRGE"
     gene            complement(7091..8374)
                     /locus_tag="VFR42_02470"
     CDS             complement(7091..8374)
                     /locus_tag="VFR42_02470"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HEU0368046.1"
                     /translation="MLSAFLIALREGVEAALVVGIILVYLARTGRSQLARHVWSGVGL
                     AAALSLAVSIALDYWRIDGDGFEGLLMLLAAAFVISMIIWMNRVARHLRKDIEQKIEN
                     YAVRAGSAAGWGIFLFVFLMVLREGVELAVILRAVELSSEGIQTWIGTILGLAAAIAV
                     GLFFFKGTLKIPVHRFFAATTIILWLVTAQLALTGLHELSEARWLPSSKGEMALIGPI
                     VRNEIFFFVFIFGAAVLLILREWLTASHAKIEAAANDAERRLLASQQRRTRNWMLAAV
                     SACLAVILVLTADFIYAKASSAPPSARPVAPASASVDDLRIPLSEVQDGNLHLYTANV
                     AGGPDGTQSFRFLIIKKPQGWGVALDACRICGAEGYRQDGQNVVCRHCGSAIYVPTIG
                     EAGGCNPIGVPFNIDGADLIVHVSALAKAFTEVPK"
     gene            8492..>9102
                     /locus_tag="VFR42_02475"
     CDS             8492..>9102
                     /locus_tag="VFR42_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368047.1"
                     /translation="MPISVTTPAAQRSRRVFGACVLLVPTAMALFAGTAGLLAQAPAK
                     QAKPAQQIKEDVKGAEKAADQGDKAAAAGKMDEALARYDAAIRLAPGDIGIRRRAAGV
                     RAAVVQKIVDQAEAKALDGRVDDAIELLYQALQIDPGNTILSQRAAEMRQMPRQYLPR
                     GDREDYDLKGPVTLKPAGGKKSINVRGDAHSAYEQVAAMFGVQV"
BASE COUNT         2050 a         2503 c         2831 g         1718 t
ORIGIN      
        1 atttgccgcg cttgctcttc gctctgccat ttgcccgagg tgccgaccac aatcgtggaa
       61 gactgcaagc tgtggccggc gcgcagaata ccatcccaga ggtcgttggc cgcgacaacg
      121 gcgaggcgaa tggacttgcc gcgtttttcc gccatggaaa gcgcctggga aaatagatgc
      181 tgttcgatgc tgccgaagag ctggtcggcc tcgaactcct gctcgccaga aggtgtgcgg
      241 cgcaggatgc gaacgtgaag aacgacgaca tcacggcggc caggctttac gcggtcaagc
      301 acattcccga gatgatacat gttgaagtga ttgctaactg gcaccaggat gttgccgggc
      361 ttggcgccga ctgcttcggg tgtgagctcg ccttcgatct caagattgaa ctgatccatt
      421 tccacatgag cgacgccggt atggcgcgcg cgcttttctg aaatggtgaa aacgacgtag
      481 agaatcactg agaaaacgcc gccagccatg gtggccacag gcttggtgaa caagttgacg
      541 aaagcaatca ggaagagtgt gagggtgatg aggccgaggc cgagcgggaa ctgcgttttg
      601 ccgatgttga aattcaaagg gacctggaat tcgcgctgac cagggttggt gtagcggagc
      661 acaaagacgg cgaggccgtt catcgcgaag ctccagatga caccgaacgc gtagaggttg
      721 gccaggaaag tgacgtcgcc gtggctgatg agaatggtga ggatctgcag cacgacgatg
      781 atgttgatga tgcgatagct ggtgccgaag cgaggatgcg gctggcgaaa ccagtcgctc
      841 aggacaccat cttcggagac acgattgagc acaccgttgg agccgacgat ggcggtgttg
      901 accgcaccgg ccaggatgac ggcaccgact acgaccacga aggcgtggaa gatgaggcgc
      961 aataagaacg ggccctcgag actcatagcg agtccgccga tgagattttc gaggaagccc
     1021 tggcggacgt cgtccggaat aatcatcacc gcgaagaagg aaaccagcga ggtaaagatc
     1081 atgctgtagg cgaaaatcac cagcccggct tttttgaggt ttacgagctt ggggctttca
     1141 atttcgcgat agacctgggc gagagattct tcgccgctca tggccaggac ggagtggcca
     1201 agcgcgatga aaatggccac gatgccaaac atatgcggga gtgagctatg cgccagccag
     1261 cccagggaag cggacgtgag atgcaggttg ctggggtacg ggaatggcgg gagcgatggc
     1321 cgacgaagaa tgatggtgta aacgcaccag ccaatgagca ggaccaccat gacggtgacc
     1381 agcttcatga ttttgagggc tttttcgctt gattccggga tgcccttggt attttcccac
     1441 cagaaataaa tcgtgatgat gatggcgaag ctggcggcga tgacgtcaat ggtgcgcgga
     1501 gagagaggcc cggtcgtcag gtgggcataa ggcaggagtt cgctcagcag tccgcccaaa
     1561 tattggccgg cggaaacacc gctgatggga ccggtcaaaa tgtagtcaaa catcagcgcg
     1621 gatacgctga actttgccag cgttccaccg agtgattgct taacggcgcg gtagacgccg
     1681 cctcgcacaa acatgctgca actttccacg tacacggcgc gtaccgcgta ggagaaaagc
     1741 atgatggcta ggatgaacca gggagcggtc ttgccgatgt agccctcggc ctcgccgccg
     1801 gcgtagtaag cggaagaggc caagtcgttc aagacgatgg cggcggcgcg ccagaaggaa
     1861 ataaaagtca gaagcgctgt cgtggcaacg atgacgcgtg gaattccgtt gccgcgacca
     1921 gccgcagccg cggggctatt gggggtagtg ctcatgtaag ggctgtaaaa agctgcaggt
     1981 taaaatgctc gcaaatttgc tgggcgaagt ccagttagcg tggaagtggg tttttggcac
     2041 aaatctcaat gcgtcagtgt ctttggaacg gaggtggtgg ccagccattt tttcaggacg
     2101 gcggcaaagc gcggttcgat gctggtgaac gccaggccca tagccttctg aggggtgaaa
     2161 taggcgaccg tggcaggagc ttcgaggaaa tcagcgtcgc ggtagatttc gatggtcacg
     2221 ctggtgccta cgggaaacgg attaggtgcc ttcacgcagc agccatgcag gctaaggtcg
     2281 gaaacttgcg acgaagtgcg ggtattctct tcgtcatcga tgatctcggc cgaggccgta
     2341 aacggaattc gtgcggcgcg acgacgttca acggacataa gcacaccttc cggaggatgg
     2401 gcagccatga gagtagcgcg gagaaaatct ggcgcgggga ataatttcaa ggaaagctac
     2461 gaaaacatca ccgggaatcg ggaaccagct gttaggcatc gcgtttaccg caaatgctcg
     2521 tgaatgtcgg cgatccggtc ttcgaccatg tggagtttgc gggagagacc ctaaatttcg
     2581 gcctcggcgc ggcggttcac gtcgtagtcg agttcgccgc ggaggcggtc tttcgtgtcc
     2641 tggcgattct ggcatgatga ccggcgctta gatggcggcc tgcatggaga ggaaaaggtt
     2701 cagcaggata aagggggtag gggtcccagg agctcttgcc gccctgcccg ccgtctgacc
     2761 aggcggcatg gttagcaatt tacgtgcgtt tggctgcgca ttcggagcag gggcaaatgg
     2821 agcgaagcaa gtcgtaggag tagatgccgg tggagtggcc gtcggtgaag gaaatctgga
     2881 tggcatactg ccctactgcc tgggccgact gcgctttggg cttgggcttg aacatgggca
     2941 gcgcgggtga cgatgcaaaa cccagcgaag actcagccat tgaatctttc ttgacgcgtt
     3001 cgtcgttgca cgtcgcgcaa ggacagtggt cgcgcaaata cacaaaatca aagtggctgg
     3061 cgtggccgtc agcccagccg atgtccacac cggcaccgct gctcacatga attttcacgc
     3121 tagcgggctt gcggcgctgg tctatcggag tggacatgga aagagtctct caaatgcgct
     3181 tcggcgctag attccgaaat tccaatttct attttccagc ttcggctttc tcgcagcagg
     3241 gccccggcgg gatgggattg ccgtgtgggc acgttttcgg atggccgagg aaggagcaga
     3301 tgcgctgatc gagttcggga gtgatgatgt gttcgaagcg gcaagcttgc tggtgcgctt
     3361 cagcgtcttc gatggcgaag gtgtcggtaa agagacgctc ggccaggcga tggcggcgga
     3421 ctacgtcgcg cgcgcgccgc atgccggatt cggtgagtcg cacttcgccc gcgtgttgcg
     3481 cgtcgaagtt accgtttcgc ttgccgttca gttccaccag ccgcgactcg gccatgcgcg
     3541 ccagcagacg cgacggcgtc aaatcagtgc ctacgatcgg caactgcccc gccggccctt
     3601 cgacgcgcag taactctgcc tgagcaggct gcccttcttc gccaaaaatc cagatttgtt
     3661 cgagcaagtg gtccacgcga atatcatcgt cggccgaaac cggcgtgcag caatcggcgg
     3721 tgttggccag cgggcaggcc atgggatgat tcgggccgtg ttgcgcggtg tcgaaggaaa
     3781 gatggccgtg cgccagttcg atacgacgat cggcttggcg ggcgatgtct ggatcgtggg
     3841 tgaccatcaa aatggtgtgg ccggatttgt gcagctcacg gaacaagttg atgacgatct
     3901 gttcgttggc ttcgtccaga ttgccggttg gttcgtcggc cagaatcagc tttgggtgat
     3961 tgatgagcgc gcgggcaata gccacgcgct gctgttcgcc gccggagagt tgcgccggaa
     4021 gatgtgtaag ccgctggccg agtccgacgc gtttcagcgt tgcggcggct tcggcctcgt
     4081 cggtggtgga atgaaaatac tgcgccagca tcacgttttc caccgcattc agatagggca
     4141 ccaggtggaa ctgttggaat acgaagccga tcttttcagc gcgatagcgc accaactggt
     4201 tttcgccgag gcgcgaaagg tccaccccat cgacgacgac gcgaccggaa gttagagtgt
     4261 cgagtccgcc cagaatattg atgagagtgg tcttgccgga gccggacggt cccatcaggg
     4321 cgacccattc gccagcggag atgttaaagg acacgccgtc gagcgcgcgc agagcgccgt
     4381 actgcttcac gagtgtgtcg atcgcaactt gtgacgcttg gcgcggcgcc gcaacgttct
     4441 ccggaacggt cgtgtgcgcg ctcattcttc gcctcggaaa acggatgcgg gacgaatgtg
     4501 gacgaggcgg cgcaacggat aggacgctag aatcgcgacg atgacggtga gtgccacaga
     4561 aacaggatac acgatgagcc gcggctgtgc ggctacaccg aacactgctt taccgagacc
     4621 gatcgagagg acaatgcctg ctgcagcgcc gattagtccg gcgcctagcc ctagcagggc
     4681 tgcttcggcc agaaaaatcc gcaagatgcg ccgcactgaa ccgcctatcg ctttcatcaa
     4741 gccgaggtcg tttttgcgct ccgcggcgat gttggtcatc gcggccatca cgcaaagcga
     4801 ggtgagaacc aacactaccg ccacggtcgc cgtcaatatc ccggaaatct tgccgtatag
     4861 ccgcgcttcc ccttccgtga actgccgaat gggccgcacg tcgacccttg ggagtttctg
     4921 ccgaagcaga tcaacagtct gatcgacttg cgccggcgtt ccgggtacgc gcaattcaat
     4981 cagactaatt tggccgacct gtcctgtgag ccgctgaatc acatccaatg gcgcgaaggc
     5041 ttgatcgtca agcgccgtgc caaagtcctg aatactctct gcgaaacaat agtcgaacga
     5101 atttccaatc ttcagacgca tctgccggtt gtcacgcacg cccagtcttt gagcgactct
     5161 ggcaccgagc tgacaatcat tgacatcaag gtagactcgc tcagcttgaa agactcttct
     5221 ggatgggaac acgtcagtga aaaggcgatc cccagtggtc tgtccattta cgaccacctt
     5281 aactgtcgga ccggcttcac tcacttttct cttctcacgg tcgcaatcaa ttggcgttac
     5341 gtcggcgact gccgagagaa gcgcgactct agaaattggt ccggcggccg tggcagacgg
     5401 aatgtcatcc gagatcgaag cgggcatcgt accgctgact tctgcggcgc ggggagcgat
     5461 cgtaacgttc gctccgaagg cacgaaactc cgccgcgacg cggcgcttgg cgtcgatttg
     5521 aagattgagt agggcggcgg tgacggcgga acctgcgcct aatgccagca gaatgacgga
     5581 caggcgaccg cggttggcga aaaccgaacg gcgcaagacg cgccagaaca tgctgcgatt
     5641 gacggagcgc cggtccggca ctgttcgctg atcgatgttc gctgctcgct cgattcccat
     5701 cattcgcccc gcagaatcgg cgccggatcg tagttcgccg cgcgacgaag cggccacgcg
     5761 ctgcccagta gggtcacgcc ggcggccagc accaggacca tgcccagcac gaggaaggag
     5821 ggagcgggcg gaacgccaaa gattcttgtt cctagaaagc gggcgagcag caatccgatt
     5881 ccatagccaa tgcagccgcc gacaagcgcg agcaacaatt gttctgcggc taacagccag
     5941 ccgacggtgg cggaggcggc gccgagcgct ttcatcaagc cgatttcgcc gcgccgttcg
     6001 atcacggacg cggcggaaga tgcacctacc gccagggccg cagccagcaa cgaagcgaac
     6061 gtcaccagcc acagcagtat acggacgcgc gtcagaattt ttccttcgcc gtcggctacc
     6121 cgacggatca cgcgcacatc cgaaccaggc aaaacttgtt taatggaata ggcaatcgag
     6181 gaaacgtagg gcgagcacga ccagcgctcc aactcgtctg ggttcatgga tttcggatcg
     6241 cgccgcgcaa aatcatcttc cggcttggtc agcgccgaga cgtacaactt gcgatactga
     6301 cccggagcgt gagccagcga ctgcgcttgc gtaagcggga ccaaaatcgc ctcgtcctcg
     6361 ggcccacccg aggaaactat tccggtgacc gtgaagggcg aggggaattt agtcacacgg
     6421 ccggttgcca gagagacttc ctcgccaact ttcaaatgat acttcgccgc gaattcagcg
     6481 ccgatgacac actcttttct gctgtccgaa aaccaccggc ccttgactcg ccaccaagga
     6541 ttcgttctcg caatgccggt tctaaactca ccgccgtcct taagcggaac gggatggtct
     6601 gaccaagttc caatgacgga gacatcccgt gtccaatctc cagactcatg ctgtccatct
     6661 ttggccgcaa acagcgaagc tgcggcatga acctcaagga ttggcgcgaa agcgatgatg
     6721 ttgttgtgcc agaagacaga cttgattttg gggaggtcgg attcgggtag gtacgcgccg
     6781 gcgttgaccg ggcgatagtc cacgccgccg atttcgaggg gcagcgaatc cgcttcaggc
     6841 gtgaccacga gatttgcgcc cagcgagcga aattccttgg ccaggcggtc gccaacatcg
     6901 agagaaacgc ttagcgtcgc cgtggctatg gccatgccca gcgaaagcgc cgcagcggtc
     6961 agcatcttgc gccgcggcct gcgggcgaag ttatcggcga tgaggcgaag aaacataaag
     7021 gcggttcaac gtggaaagtt agatgttcac agtaaaatca aagccgtttc gtaattttcg
     7081 acttcccttc tcacttcggc acttccgtga aggccttcgc caaggccgat acatgtacta
     7141 tgagatcggc gccgtctatg ttaaagggca cgccgatggg attgcagccg cccgcttcgc
     7201 caatggttgg gacataaatc gctgagccac agtgacggca gaccacgttc tggccgtcct
     7261 ggcgatagcc ttcggcgccg caaatccggc atgcatccag cgcgacaccc cagccctggg
     7321 gcttcttgat gatcaaaaat cgaaacgatt gagttccgtc cgggccgcct gcaacattcg
     7381 ccgtgtacag gtgcaaattg ccgtcctgca cttcactgag aggaatccgc aaatcatcaa
     7441 cagaggcact cgccggagcc accggacggg ccgaaggggg cgcggagctt gccttggcgt
     7501 agatgaagtc cgccgttagc accagaatta ccgcaaggca tgccgaaact gccgcgagca
     7561 tccagtttcg cgtgcgacgc tgctgcgaag ccaacagcct tctttccgca tcgttggccg
     7621 ccgcttcgat ctttgcgtgc gaagcggtca gccactcgcg caaaatcagc aggacggccg
     7681 cgccgaaaat aaacacaaag aagaaaatct cattgcggac aatcgggccg atcagcgcca
     7741 tttcgccttt gctagagggc aaccagcgcg cttcgctcaa ctcatgcaag ccggtcaggg
     7801 ccagttgcgc tgtgaccagc cacaaaataa tcgtggtcgc ggcgaaaaag cggtgtacag
     7861 gaatcttcaa cgtgccctta aagaagaaaa ggccgacggc aattgccgcg gccaagccga
     7921 ggatggttcc gatccaggtc tggatgcctt cgctggaaag ctcgaccgcg cggagaatta
     7981 cagccaactc gacgccttcg cgcaggacca tgagaaaaac aaagaggaag ataccccagc
     8041 cggcggctga accagcacgc accgcataat tttcaatttt ttgctcgata tcttttcgca
     8101 agtgccgcgc aacgcggttc atccagataa tcatcgaaat gacgaacgct gctgcgagca
     8161 gcatcagcag gccttcgaag ccgtcgccgt cgatgcgcca atagtcgaga gcgatcgaca
     8221 cggccaggct gagagcggcg gcaaggccta cgccagacca gacatggcga gccagctggg
     8281 aacgaccggt gcgggccagg tacaccaaga tgatgccgac gaccagggcg gcttcgacgc
     8341 cttcacggag agcaatgaga aaagcagaga gcaagtggat gattccagtt gtaacttact
     8401 tgcaactaca attcaagttt accgccagag acccggcagg tcaatcggac taatctagtc
     8461 ctatttgttc aggatagaat acacgagtct tatgcccatt tcagtgacta cgcccgccgc
     8521 tcaacgaagc cgcagagtct ttggcgcttg cgttcttcta gtccccacag ccatggcgct
     8581 tttcgccggc acggctgggt tgttggcgca agcgcccgcg aagcaggcca aaccggctca
     8641 acaaatcaag gaagatgtga agggagcgga aaaggccgcc gaccagggcg acaaagcggc
     8701 ggcggcgggc aaaatggatg aagcactcgc tcgttacgac gccgcgatac ggctggcacc
     8761 gggtgacatt ggcatacggc ggcgggcagc gggagtccgt gcagcggtgg tgcagaaaat
     8821 cgtggatcag gcagaggcca aggcgctaga tggtcgcgtg gatgatgcca ttgaactgct
     8881 ctatcaggcg ctgcaaatcg atcccggcaa taccatattg tcgcaacgcg cagcggagat
     8941 gcggcagatg ccgaggcaat acctgccgcg cggcgaccgc gaagattacg atttgaaagg
     9001 accggtgacg ctcaaaccgg cgggcggcaa gaaatccatc aatgtgcgcg gtgatgcgca
     9061 cagtgcatac gagcaagtgg cagccatgtt cggcgtgcag gt
//
LOCUS       DASTPM010000138         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_581048, whole genome shotgun sequence.
ACCESSION   DASTPM010000138 DASTPM010000000
VERSION     DASTPM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581048"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VFR42_02480"
     CDS             <1..1258
                     /locus_tag="VFR42_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0368048.1"
                     /translation="TEVLKSGARGSTVSASQRVRSSLAIAEIGLAALLIIAAGLLVRS
                     FWALSHVNPGFRAEHVVTARITPNQSFCNDPSRCLAFYRNVLEQVRSSPAVSEVALVN
                     TLPLNGRVSKRSLQIENLTSSTGEVILPLFWLHVVTPDYFRVLGIPLQSGRWFSQADE
                     SVNAAIAVLSAESAERFWPGQEAVGKHIRFAGDTEWRTVVGVIGDVRDYDLQRTIPDY
                     MRGSVYVPYNLKATGELRQIPSEMTIAVRTAESDSQVGTMLRAVVSVANPEVPVSELK
                     TMRAVFVDAAATPASTASLFIAFAALALVLGMVGIYGVLAFLVSRRTREIGIRLAMGA
                     QRQNVFWMVLKEGAKFYVAGVTLGLIGAFATTRLLASELYGVSAFDPLTYAVVAVVMA
                     LITLLACCIPARRAMRVDPLVALRCE"
     gene            1268..>2464
                     /locus_tag="VFR42_02485"
     CDS             1268..>2464
                     /locus_tag="VFR42_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368049.1"
                     /translation="MDTLVQDFRYGLRMLLKAPAFVVIAVLTLALGIGANTALFSIVS
                     SVLLSPLPFHEPDRLMAVYSSNREFRNASISYPNFLDWVRNNRSFSDLAAFRQDSFEL
                     TGMGEPERLKVEMVSANFFPLLGVRPVIGRQFLVEEDQVGAAPVVLISAGVWQRKLGA
                     TQDLAGKTLHLNGTSYSVIGVIPASFHYYGGNFYRNTDIFVPIGQWNDPTFRDRRTGM
                     GTNAVGRLKPGVTFAQARSDMDAVAANLAMAFPDVDRDSGISLLPLRQNVVGEIRPVL
                     LVLFASVGFVLLIACANVANLLLARSTGRAREFSVRAALGAGRIRIIRQLLTESVLLS
                     LLGGALGLLLAAWSTRAGLAALPEALPRAEEIRLNLPVLLFTVGLSVLIGILFGLVPA
                     LRTSRAD"
BASE COUNT          403 a          795 c          676 g          590 t
ORIGIN      
        1 cacggaggtt ctgaagtccg gcgcgcgcgg gtccactgtt tccgcgtcgc agcgtgtccg
       61 aagctctttg gccatcgccg aaatcgggct ggccgccttg ctcatcattg ccgccggact
      121 gctggtcagg agtttctggg cgctctccca cgtgaatccc ggattccgtg cggagcatgt
      181 ggtgactgct cgcatcacgc ccaatcaatc gttctgcaat gatcccagcc ggtgcctggc
      241 cttctatcga aacgttttgg agcaagtgcg atcctctccg gcagtcagcg aagtagccct
      301 ggtgaacacg cttcctttga acggaagagt cagcaagcgc tcgcttcaaa ttgagaatct
      361 gaccagttcg accggcgaag tcatcctgcc tcttttttgg ctccacgtgg tcacccccga
      421 ttacttccgc gtcctgggca ttcctctgca gtccggccgt tggttcagcc aggcggacga
      481 gtccgtaaat gccgcaatcg cggtgctaag cgccgaatcg gccgaacgat tctggccagg
      541 gcaagaagcg gtcggaaaac acatccggtt cgccggagac actgaatggc ggacggtcgt
      601 tggtgtgatt ggcgatgtgc gcgactacga tttgcagcgg acgattcccg actacatgag
      661 gggctctgtg tacgttccct ataacctcaa agcaaccggc gaactccgcc agattccttc
      721 ggagatgacg atcgctgtgc ggacggcgga aagtgattca caagtcggaa ctatgctgcg
      781 cgcggttgtc tccgttgcca atcccgaggt tccggtgagt gagctgaaga ccatgcgcgc
      841 cgtttttgtc gatgccgcgg ccacccccgc ttcgaccgca tccttattta tcgctttcgc
      901 ggctttggcc ctggtacttg gcatggtggg gatttatggc gtcctggcgt ttctcgtttc
      961 acggcgcact cgcgaaatcg gcatccggct cgccatgggt gcgcagcgcc agaatgtgtt
     1021 ctggatggtc ctcaaggaag gcgccaagtt ctacgttgcg ggcgtgactc tgggtctcat
     1081 cggggctttc gcaaccactc gtttgctggc tagcgaactc tacggcgtca gcgctttcga
     1141 cccgctaacc tatgccgtag tcgccgtcgt catggctctc atcaccctac tggcatgctg
     1201 cattccagcg cgacgcgcca tgcgcgtcga tcccctggtc gctcttcgct gcgaataagg
     1261 agaactgatg gacacccttg ttcaagattt ccgatacggc ctcagaatgc tgctcaaggc
     1321 gcccgccttt gtcgtcattg ccgttctgac tctggctctc ggtatcggcg caaataccgc
     1381 tcttttttcc atcgtcagca gcgttctcct tagcccgctg ccttttcatg agcccgatcg
     1441 ccttatggcg gtctactcga gcaatcggga attccgcaac gcttcaatct cctatcccaa
     1501 tttcctcgac tgggttcgca acaaccgcag cttttccgac cttgccgcct ttcgccagga
     1561 tagcttcgaa ctgaccggca tgggcgaacc cgaacgcctc aaagtcgaaa tggtttcggc
     1621 caatttcttt ccgcttctcg gcgtccgacc ggtcatcggc cgtcagttcc tcgtcgaaga
     1681 agaccaggtc ggcgccgccc cggttgttct tatcagcgcc ggagtctggc agcgcaaact
     1741 cggtgccacg caggatctcg ccggcaaaac gctccacctc aatggcacct cgtactcagt
     1801 cataggcgtg attccggcca gttttcacta ctacggcggc aacttttatc gtaataccga
     1861 cattttcgtc cccatcggcc agtggaacga tcccactttc cgcgatcgcc gcaccgggat
     1921 gggcaccaac gccgttggcc gtctcaagcc cggcgtcacc tttgcccagg ctcgctctga
     1981 tatggacgcg gtcgccgcca acctcgctat ggctttcccc gacgtggacc gcgactccgg
     2041 catcagcctc ttgcctctcc gtcagaatgt cgtcggagaa atccgtccgg tcctcctggt
     2101 tctctttgcg tcggtgggat tcgtacttct gatcgcgtgc gccaatgtcg ccaatcttct
     2161 gctcgctcgt tccactggcc gcgcacgcga attcagtgtt cgcgcagccc tgggcgccgg
     2221 ccgcatccgt atcatccgtc agctcctgac ggaaagtgtc ctgctctcac tgcttggcgg
     2281 agcactcggc ttgcttctcg cggcctggag cacccgcgct ggcctggccg ccttgccgga
     2341 ggcccttcct cgcgcggagg agatccgttt gaacctgccg gttcttcttt tcacggttgg
     2401 gctctccgta ctcattggaa ttctctttgg tttggtcccc gctctgcgca cttcccgtgc
     2461 ggat
//
LOCUS       DASTPM010000139         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1100265, whole genome shotgun sequence.
ACCESSION   DASTPM010000139 DASTPM010000000
VERSION     DASTPM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100265"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VFR42_02490"
     CDS             complement(<1..1160)
                     /locus_tag="VFR42_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU0368050.1"
                     /translation="MRWQTAKPPNLWVFGIGSGIALFVVVMTALFFVRRYFRNARNAH
                     KEEEPQEARPQTPSAFMAASMQGVIQKLRDQEKELERLHRSEKERAEHTERLSEEVTR
                     NMPAGLLVVNANGIISSANPAAEQALGIHGLGFRRYSEALREGSELTRLVAECLSEGK
                     IFRREQVEHATPAGERRHLGVTISPIRKGQEKISGALCLLSDLTELSALQQQMQLKEN
                     LAALGELSAGIAHEFKNALATISGYAQMIRSETKPGTELREHGEKILDQTRALAHVVT
                     EFLKFARPLDLADEQVTLRPLIDRILMEVSEVVPEVPFTCEGEFGDVSGDDALLRQAI
                     LNLARNAAEAAADNPGGGRVIIRGEIEQTGAMQGQRISVSDNGPGIPAEALT"
     gene            complement(1183..2082)
                     /locus_tag="VFR42_02495"
     CDS             complement(1183..2082)
                     /locus_tag="VFR42_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU0368051.1"
                     /translation="MIVFIFLFGIVIGSFLNVCILRIPEELSIVMPASRCPQCHALIK
                     WYDNVPVFGWMWLRGKCRNCGTAISAMYPLVELVTGLLFVACYLEFGLTQAMVKWVFF
                     MCLLMVLSITDLRVRLLPDLVNWPGFAAGLFLSAMVPSEDGTALWICRSLFRAVPARP
                     VLGLLDSLLGAAFGSFLLWGVAAAYKRVRGREGMGMGDVKMMALVGAFLGVRGAFLTI
                     LVGTLLGTVIGVTVVLVLYAAGWHRKLAERASRRGVGSVNALRWAIASQYQLPLGTFL
                     GIAGVMVMFYGGEIMVRWSQLFR"
BASE COUNT          469 a          811 c          524 g          405 t
ORIGIN      
        1 ttggtcaagg cttcggcggg aattcccggt ccattgtcgg aaacggaaat tcgctggcct
       61 tgcatggcgc ccgtttgttc gatctcgccg cggataatga cgcgtccgcc gccgggattg
      121 tccgctgcag cctcggcggc gttgcgcgca aggttcagaa tggcttggcg tagcagggcg
      181 tcgtcgccgg aaacatcgcc aaattctccc tcgcaggtaa agggcacctc gggcacgact
      241 tcggacactt ccatcagaat ccggtcgatc aacggccgca gcgtcacctg ctcatcggcg
      301 agatcgagcg ggcgcgcgaa tttaagaaat tcggtcacga cgtgagccaa cgctcgcgtc
      361 tggtccagga ttttttcgcc gtgttcgcgc aattccgttc cgggttttgt ctcgctgcgg
      421 atcatttgcg cgtagcctga aattgtggcc agcgcgttct tgaattcgtg cgcgatgcct
      481 gcggatagct cccccagcgc cgccaggttt tccttcagct gcatctgctg ctgcaaagca
      541 gaaagctcgg tcaaatcgct cagcaggcaa agcgccccgc tgattttttc ctgccctttg
      601 cggatcggcg aaatcgtcac tcccaggtgc cgccgctcgc ccgcgggcgt ggcgtgctcc
      661 acctgttccc ggcgaaaaat cttcccttca ctcaaacatt ccgccaccag ccgcgtcaat
      721 tccgagccct cgcgcaatgc ctcgctgtag cgccggaatc ccaatccatg aatccccagc
      781 gcctgctccg ccgccggatt cgcgctggaa ataattccgt tggcgttcac caccagcaat
      841 cccgccggca tgttgcgggt cacttcttcg ctcagccgct cggtgtgctc ggcgcgctct
      901 ttctcgcttc gatgcagccg ctccagctct ttttcctgat cccgcagctt ctggatcact
      961 ccctgcatcg atgccgccat gaatgccgag ggagtttgcg ggcgcgcctc ttgcggttcc
     1021 tcctccttgt gcgcgtttcg cgcgttgcga aaatagcgcc gcacaaaaaa caacgccgtc
     1081 atcaccacca caaacagcgc gatgcccgat ccaatcccaa acacccaaag attgggcggc
     1141 ttggccgttt gccacctcac gaattggacc agtccgttca tctcatctaa acagttgcga
     1201 ccaccgcacc attatctctc caccgtaaaa catcaccatc actcccgcga ttcccagaaa
     1261 cgttccgagc ggcagctgat attggctggc gatcgcccag cgcaaagcat tcacgctgcc
     1321 caccccgcgt cggctggccc gctccgctag cttgcgatgc catcccgcgg catacaacac
     1381 cagaaccacc gtgacgccaa tcaccgtccc cagcaacgtt cccaccagga tcgtcagaaa
     1441 cgccccgcgc acgcccagaa acgcccccac caacgccatc atcttgacgt cccccatccc
     1501 catcccttct cgcccgcgca cccgcttgta cgccgccgcc actccccaca aaagaaagct
     1561 gccgaacgcc gcgcccagca gggaatcgag cagccccagc accggccgcg caggcaccgc
     1621 gcgaaatagc gacctgcaaa tccagagcgc cgtgccatcc tccgagggaa ccatcgccga
     1681 taagaacaaa cccgctgcga accccggcca gttcaccaga tccggtagca gccgcacccg
     1741 caagtccgta atcgacagca ccatcagcaa gcacatgaag aaaacccact tcaccatcgc
     1801 ctgcgttagc ccaaattcca gatagcacgc cacgaacagc agcccggtaa ccagctccac
     1861 cagcggatac atcgccgata tcgccgtccc gcagttccgg catttcccgc gcagccacat
     1921 ccacccaaat accggcacat tgtcatacca cttgatcagc gcgtggcact gcgggcagcg
     1981 cgaagccggc ataacaatcg acagctcctc cggaatccgc aaaatgcaga cattcaggaa
     2041 gctgccaatc acaatcccaa aaagaaaaat gaaaacaatc accaaggtaa gcgtagccgt
     2101 cccacggccc ctctacccaa tctcccacat cgcccgaccc cactcaacgc aagcaccgca
     2161 ccccagtgcc ccaaccctaa cacctctttc tgcgaacctc tgcgttctc
//
LOCUS       DASTPM010000140         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_984877, whole genome shotgun sequence.
ACCESSION   DASTPM010000140 DASTPM010000000
VERSION     DASTPM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984877"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFR42_02500"
     CDS             <1..190
                     /locus_tag="VFR42_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368052.1"
                     /translation="ILKKLGATQIRLLSNNPEKVRQLEAAGVNVVERVPCQPRISKIS
                     RAYLQTKKKKMGHLLKGL"
     gene            213..794
                     /locus_tag="VFR42_02505"
     CDS             213..794
                     /locus_tag="VFR42_02505"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0368053.1"
                     /translation="MTPALETERLFLKPLALADAKPIQKLFPRWEIVRYLNAAIPWPY
                     QADGARTFIERVLPEMERGESFFWTLRVKSAPQQLIGVISLFLKKEENRGFWMDPEWQ
                     GKGLMTEACDAVTAFWFDVLKQPVLRAPKALVNEASRRISVKQGMRIVSREERDYVIG
                     RLPSEIWEITAEEWRARQRMSLGHKREPDTRMS"
     gene            complement(791..1009)
                     /gene="thiS"
                     /locus_tag="VFR42_02510"
     CDS             complement(791..1009)
                     /gene="thiS"
                     /locus_tag="VFR42_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU0368054.1"
                     /translation="MQTVETISIIVNGEPKTAPGGATVTQLLESLALRPGRVAIERNL
                     QILPRSEWATTQVASGDRYEIVQFVGGG"
     gene            complement(1018..1629)
                     /locus_tag="VFR42_02515"
     CDS             complement(1018..1629)
                     /locus_tag="VFR42_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368055.1"
                     /translation="MVFGHNSNVAIGPDTVHVQTEDRGLAHAVIDTTVHWKGRVLHRR
                     TNNYKDLLPLDPGKEAELKLRVDEQHRAVVEQLRSGALHVAIPKTPAPPAPLPPKPAA
                     SAGLKVDLTNSRTWLSGRNATLQLLVCDGNGNPVANAAAKARVEGAASPFESAAETSP
                     AGIATLRFEMPRLTGDEPALVIEVAHNGAKGHLRFQLRAKPRL"
     gene            1773..2144
                     /gene="ndhC"
                     /locus_tag="VFR42_02520"
     CDS             1773..2144
                     /gene="ndhC"
                     /locus_tag="VFR42_02520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU0368056.1"
                     /translation="MSSSGLQTYAPLVLHLLIAAILAGLISSLSVLIGWRRPNRAKQQ
                     AYECGMEPTGDAREPFSVKFYLVAMVFILFDVEAIFLYPWAMVFRSLSWYGFSEMMMY
                     IAILLVGYFYLWKKGALDWHK"
     gene            2169..>2484
                     /locus_tag="VFR42_02525"
     CDS             2169..>2484
                     /locus_tag="VFR42_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU0368057.1"
                     /translation="MAESDATSVIVEKLRGWNANAVSEVIEFHGETTVVVPRELLRET
                     AEYCRSQLEFNYLSDATSVDRFPVEPRFEVSYHLVSIPKRVKIRLRTRVSGADPTVDS
                     VVP"
BASE COUNT          516 a          658 c          763 g          547 t
ORIGIN      
        1 aatcctgaag aagctgggcg cgacacagat ccggctgctg tccaacaatc cggagaaggt
       61 gcggcaactg gaagcggccg gggtaaacgt ggtggagcgc gttccctgtc agccgcgcat
      121 ttcgaaaatt tcgcgcgcct acctgcaaac caagaaaaag aagatggggc acctgctcaa
      181 gggactgtga attacgtttt gtcagccgcg cgatgactcc cgcacttgaa accgagcgtc
      241 tatttctaaa gccgttggcc ctcgcggacg ccaagccgat ccagaaactg tttccgcgct
      301 gggaaatcgt gcgctatctg aacgccgcga ttccctggcc atatcaggcc gatggcgcga
      361 ggacgttcat cgagcgagtt ctgccggaga tggagcgggg cgaatccttt ttctggacac
      421 tcagagtaaa gagcgcgccg cagcagctga tcggcgtgat cagtcttttt ttgaaaaaag
      481 aagaaaatcg cgggttctgg atggatccgg aatggcaggg taaaggcctg atgacagaag
      541 catgcgacgc ggtgacagcg ttctggtttg atgttttgaa acagccggta ttgcgcgcgc
      601 cgaaagcgct tgtgaacgaa gcgtcgcggc ggatttccgt gaaacaagga atgcgcatcg
      661 tttcgcgcga agagcgagac tacgtgatcg ggcgcttgcc ttcagaaatt tgggaaatca
      721 ccgcggaaga gtggcgtgcc cgccagcgaa tgagcttggg acacaagcgc gaaccagaca
      781 ctcgcatgag ttagccgccg ccgacaaact ggacgatttc atagcggtcg ccggatgcga
      841 cttgcgtcgt ggcccattcg gatcgcggca ggatttgcag attgcgttca atggccacgc
      901 ggcccgggcg cagagccagc gattcaagaa gttgcgtaac tgtggctccg ccgggcgcgg
      961 tcttgggttc gccgttgacg atgatggaaa tggtttcgac ggtttgcatt gctttgctca
     1021 gagcctaggt ttcgcccgca actgaaaacg cagatgaccc tttgcgccat tgtgtgcgac
     1081 ttcaatcacc aaggcgggct cgtcgccagt gagcctgggc atttcgaatc gcagcgtggc
     1141 gattccggcc gggcttgttt ctgcggcgga ttcgaacgga cttgccgcgc cttcgacacg
     1201 ggcttttgcc gccgcattcg ccacgggatt tccgtttccg tcgcagacca ggagttgcag
     1261 cgtggcgttg cggccggaaa gccaggtgcg agagttggtc agatccacct ttagcccggc
     1321 agaagcagcc ggctttggcg gtagcggcgc aggcggcgct ggggttttcg ggatggcaac
     1381 gtgcagcgcg cccgagcgaa gttgttccac cacggcgcgg tgctgttcgt ctacgcgcag
     1441 tttcagttca gcttccttgc cagggtcgag cggaagtaag tccttataat tgttcgtccg
     1501 gcgatggagg acgcggccct tccaatgtac ggtagtatca atcaccgcat gcgccaagcc
     1561 acgatcctcg gtctgcacgt gaaccgtgtc cggtccaatc gcgacattgc tgttgtggcc
     1621 gaagaccatg cggaagctgt cctaaaaagc taccccgaaa gctagtttat cgcgcagcga
     1681 aaactcaagg ttttctaatc agcgctttcg agagaatttg cggcgcgcga tttgaccgaa
     1741 cgccaactcc tcgttattat accgcttcgc ctatgagtag ttcaggtcta caaacctacg
     1801 cgcccctggt tttacacttg ctgatcgccg ccattttggc gggactcatt tcatccttgt
     1861 cggtactgat cggctggcgg cggccaaacc gcgccaaaca gcaagcttac gagtgcggca
     1921 tggagccgac cggcgacgcg cgcgagcctt tttccgtaaa attttattta gtggccatgg
     1981 tgttcatcct tttcgacgtg gaagccatct ttttgtatcc gtgggcgatg gtttttcgct
     2041 cgctgagctg gtacggattc tcggagatga tgatgtacat cgccatccta ctggtcgggt
     2101 acttttatct ctggaaaaag ggcgcgctgg actggcataa gtagccagcc gggcaaatcg
     2161 gaaaaattgt ggcagagagc gacgctacga gtgtaatagt cgagaagctg cgcgggtgga
     2221 acgcgaacgc ggtctcggaa gtcattgagt ttcatggtga aacgacggtg gtagtgccgc
     2281 gcgaactgct gcgcgagacg gcagaatatt gtcggagcca gctggaattc aattatctgt
     2341 cagatgctac ctccgttgac cgcttccctg tcgagccgcg ttttgaagtg agctatcacc
     2401 tggtttccat ccccaaacga gtgaagattc ggctgagaac gcgggtcagc ggagccgatc
     2461 cgactgtcga ttcggtggtg ccgg
//
LOCUS       DASTPM010000141         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_33439, whole genome shotgun sequence.
ACCESSION   DASTPM010000141 DASTPM010000000
VERSION     DASTPM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33439"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFR42_02530"
     rRNA            complement(<1..765)
                     /locus_tag="VFR42_02530"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1907..>4271
                     /locus_tag="VFR42_02535"
     CDS             1907..>4271
                     /locus_tag="VFR42_02535"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0368058.1"
                     /translation="MRRTQFLKSLSLLALLFLFASDAARAQSIVTGAISGTVTDSSGA
                     IIADAKVTLINESTQETQSVSTDESGVYQFPLLKPGRYSVSVEKEGFRRLTQKTDVLL
                     GQTANSNVRLEVGETSQSVEINESAPLLQTEDGNITSNVDLRTLQNIPNPGGDLSYIA
                     QISPGVTMNTSNGGGFGNFTAFGLPATANLFTINGNDYNDPFLNLNNSGASNLLLGSN
                     EVQEVAVVSNGYTGQYGRQAGAQVDYTTKSGGNSFHGNAVYYWNGSALNANDFFLNQA
                     GEARPFENNNQWAASFGGPIKKDKAFFFINTEGLRYTFGSSNEVFVPTAELQNFILAT
                     SLPANNAAAIPFYQQMFALYNNAPGAANAQPVAGDSCGSLDTVFSGPCLASYRVSVPN
                     GNREWLISGRVDYNLSQNDKIFGRVRFDRGNQPTYTDPINPVFNIQSNQPQNEGQLNY
                     THVFNPRVVNNFIGSVLYYSALFQSPNLSSALSAFPYILNISDANLTPLGSGSGSFPS
                     FAIFPQGRNVTQWQLVDDLSIARGKHTLKMGANFRRDDVSDHTASEGSYPTISSDMTQ
                     FADGVAGSIAQNFALRTTQPLAFYSMGLYFQDEFRVNSHLKLTLALRADRNSGGTCSS
                     DCVARASAPFAELSHDATIPYNQMMTTGTNQILPAVERVVFEPRAGFAWSPHGDKTVI
                     RGGVGLFTDLYPGTILNQFTTNFPQVTRFSLSQLGTIDTMSNPASAAAIIAQCNAAFQ
                     NTFNGGGTVTNFLNAAPAGCATPDLNDVVGRLLNPKYLEWNLQVQHTV"
BASE COUNT          956 a         1344 c         1033 g          938 t
ORIGIN      
        1 tatctaatcc tgtttgctcc cctagctttc gttcctcagc gtcagtagtg gtccagcgcg
       61 ccgctttcgc cacgggtgtt cctgcagata tctacgcatt tcaccgctac acctgcaatt
      121 ccacgcgcct ctcccacact caagcacagc agttttggaa gcagcctctg ggttaagccc
      181 agagatttca ctcccaactt gccgcaccgc ctacgaaccc tttacaccca gtaattccga
      241 acaacgctgg ccccctacgt cttaccgcgg ctgctggcac gtagttagcc ggggcttctt
      301 atatccgtac cgtcaatccc ttgcggggtt tcgtccggat cgaaaggggt ttacacaccg
      361 aaatgcttca tcccccacgc ggcgtcgctg catcaggctt tcgcccattg tgcaagattc
      421 cccactgctg cctcccgtag gagtctggac cgtgtctcag ttccagtgtg gctgatcatc
      481 ctctcagacc agctaccgat caaagccttg gtgagccgtt acctcaccaa ctagctaatc
      541 gggcgcgggc ccctccttcg gcgccttgcg gctttcatcc ttgcggatct tatgcggaat
      601 tagctccgct ttcgcggagt tattccccac caaagggcag gttacccacg tgttacgcac
      661 ccgtgcgccg tgcgccagtt gtattgctac aaccgcgcac tcgacttgca tgtgttaagc
      721 acgccgccag cgttcgttct gagccaggat caaactctcg tttgaagcct gtcgcttagg
      781 cgaaaaaaaa tgccctccga ccgaagtcga agagccagcc tacctaagca aattgttgag
      841 tcgctcgatt gttactcagt cattcgggct cgaataggcc gaatgactcg aacggattct
      901 ggcaaatcat attcagttgt ctatgaacga tagcctcccc tgtccagcag agtggcattc
      961 cgaagaacct gatgagtata gggaacgcct agcggattgt caacacatag aaaaaacttt
     1021 tttcgatttt tccacaagat tcacttcctt cctgtaactc gctgataact tacgcggata
     1081 ccagcctgca cccaagactt ttcctcgccg ggcactccaa gtgcagcgtc gcataatcgc
     1141 aaatcttgcc gctcgaactg gattctctta cgatagccct ccgcctgcac actactccaa
     1201 tccggaccat cgacgcgaag gggagacggg tatcgaccgc cctcgcgatt gggcagcgat
     1261 ccttcacacg agtcctctgc agtgatggtc ctgtcccgcc tccgagccat cgcgctaggc
     1321 gggatcgttc acgattgttt ttctggaagc cgccgagtcc taagccaagg aagagacttc
     1381 ctgcgcgcac cacgcatcac aatggcaagc ctcggtgtag ggaaacccgc gcgggcaaga
     1441 gtctgccggg ttctctcgat tccaaaaatt taattattca taaagataac tccagcattt
     1501 catacctttt gataactaca attttttgaa gaaatctgtg gtttagcaaa gcgtactagc
     1561 acccatccaa aaatcagaat tccgtaatac ctgcttaatc aagctattac aaagtttgcg
     1621 ccgactggca cggttcttgc agtcttactt ttcgccaagg tagttcttcg agtgggtact
     1681 gcaaagccct aacaggaaag tcgtcacgcc tcaggaaagt agcgacgggc tggcagtaat
     1741 aaaaacatag ctggtggtgg cggaggaaga gcgtgaattg gggttttcac gccggcgcag
     1801 tggtgtgcgg gctcgcgaag tttgaaaccg agctgcacga ggataaagca tttttgctgg
     1861 agaggaacta cgagtgggaa caccaactca aggagaacga aaataaatgc gaaggacgca
     1921 gtttctcaaa tcgctgtctt tgctggcgct gctttttctt ttcgctagcg acgccgccag
     1981 agcgcaaagc atcgttaccg gcgctatcag cggcaccgta accgactcat ctggggcgat
     2041 catagccgac gccaaagtca cactcatcaa tgaatcgaca caggaaacgc aatcggtttc
     2101 gaccgacgaa tccggcgttt accaattccc gttgctaaaa ccggggcgct actcggtctc
     2161 ggttgaaaag gaaggcttcc gccggctcac gcaaaaaacg gacgttctcc taggccagac
     2221 tgccaacagc aacgttcgtc tcgaagttgg cgaaacttcg cagtccgtcg agatcaacga
     2281 atccgctccg ctgctgcaga ccgaagacgg caatattacg tccaacgtgg acctgcgcac
     2341 gctgcagaac attcccaatc ccggcggcga cctcagttac atcgcgcaaa tttcccccgg
     2401 cgtgaccatg aacacctcga atggaggcgg cttcggcaac ttcactgcat tcggacttcc
     2461 cgccacagcg aatcttttca ccatcaacgg caacgactac aacgatcctt tcctcaactt
     2521 gaacaactcg ggagccagca acttgctcct cggctccaat gaagttcagg aagtcgcggt
     2581 ggtcagcaac ggctacaccg gccagtatgg ccgccaagcc ggcgcgcagg tggattacac
     2641 caccaagtcc ggcggcaact cgtttcacgg caatgccgtt tactactgga atggcagcgc
     2701 gctcaacgcc aacgattttt tcctgaacca ggcgggagaa gcgcgcccct ttgaaaacaa
     2761 caaccagtgg gcagcttcgt ttggcggccc catcaagaag gacaaagcct tcttcttcat
     2821 caacacggaa gggctgcgct acaccttcgg ctccagcaat gaagtattcg tgccgaccgc
     2881 ggaactgcaa aactttattc tcgctaccag cttgccggcc aataatgccg cggcaattcc
     2941 cttctaccag cagatgtttg cgctgtacaa caacgcgccc ggagcggcaa acgcacagcc
     3001 ggttgctggc gattcgtgcg gtagcctgga caccgtcttt tccggcccat gtctcgcgtc
     3061 ctaccgcgtc tcggttccca atggcaaccg cgaatggctc atcagcggcc gtgtggacta
     3121 caacctatcg caaaacgaca aaatctttgg tcgcgtcaga ttcgatcgcg gcaatcagcc
     3181 cacctacacc gaccccatca atccggtctt caacattcag agcaaccagc cgcagaacga
     3241 aggtcagttg aattacacgc acgtcttcaa tccgcgtgtc gtcaacaact tcatcgggtc
     3301 tgtcctctac tactccgccc tgttccagag ccccaatctc agcagcgcac tctcggcgtt
     3361 tccttacatt ctcaacatct ccgacgccaa ccttaccccg ctcggttcgg gctcaggttc
     3421 atttccttcc tttgcgattt ttccccaggg ccgcaatgtc acccagtggc aattggtgga
     3481 tgacctctcc atcgcgcgcg gcaagcacac tctcaaaatg ggcgcaaact tccgtcgcga
     3541 tgacgtcagc gaccacaccg ccagcgaagg tagctatccg accatcagct ccgacatgac
     3601 gcaattcgcc gacggcgtcg cgggcagcat cgcccagaat ttcgcgctgc gcaccacgca
     3661 gcccctggct ttctacagca tgggcttgta tttccaggat gagttccggg tgaattcgca
     3721 cttaaagctg acgctcgctc tgcgcgctga tcgcaactcc ggcgggacct gctcctccga
     3781 ctgtgtggcc cgcgccagcg cacctttcgc ggagttgagc catgatgcaa ccatccccta
     3841 caaccagatg atgaccaccg gcaccaacca gatccttccg gccgtcgaac gtgttgtctt
     3901 cgaaccgcgt gccggctttg cctggagccc gcacggcgac aagaccgtca tccgcggagg
     3961 cgtcggcctt ttcacagacc tttatcccgg cacgatcctg aatcagttca ccacgaactt
     4021 tcctcaggtg actcgtttct ctctctctca gcttggcacg atcgatacca tgtccaatcc
     4081 ggccagcgcg gcggccatca ttgcgcagtg caacgccgcc ttccagaaca ctttcaatgg
     4141 cggcggcacg gtcactaatt tcctgaacgc cgcacctgcg ggatgcgcga cgcccgacct
     4201 gaacgatgtc gtcggccggc tgctgaatcc caagtatttg gaatggaacc tgcaagttca
     4261 gcacaccgtc g
//
LOCUS       DASTPM010000142         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_292965, whole genome shotgun sequence.
ACCESSION   DASTPM010000142 DASTPM010000000
VERSION     DASTPM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292965"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..667
                     /locus_tag="VFR42_02540"
     CDS             125..667
                     /locus_tag="VFR42_02540"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0368059.1"
                     /translation="MSELAADQAYVLVQNALLGTIKSESRTTKSVLAAVPNSNLDYRP
                     DSCAKTAIELVRHIAVSENRFLECVSTGIYDTTSPALPANITAPADIAAWYEQSFARN
                     FDAVSKLSGEQLIKMVNFRGMFERPAYFFLQLCLVHSVHHRGQLSTYLRPMGSKVPAI
                     YGESYDSAEAKKAAAAQAQA"
     gene            complement(987..1763)
                     /locus_tag="VFR42_02545"
     CDS             complement(987..1763)
                     /locus_tag="VFR42_02545"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU0368060.1"
                     /translation="MSDQLVIAGRAFSSRLIVGTGKYRSFQEMARAHAASGADMVTVA
                     VRRVNLTDKSKESLLDFIPRDKIFILPNTAGCYTADEAVRTARLAQEVGLSNWVKLEV
                     IGDQQTLYPDTEQLIEATKILVKEGFVVLPYTNDDLIAARKLIDAGAAAVMPLAAPIG
                     SGLGVQNPAALRILRERITEVPLIVDAGVGTASDAAVAMELGADGILMNTAIAEANDA
                     ELMAEAMRDATSAGRKAYLAGRMPKRLYASASSPIAGVVR"
     gene            complement(1825..>2745)
                     /locus_tag="VFR42_02550"
     CDS             complement(1825..>2745)
                     /locus_tag="VFR42_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0368061.1"
                     /translation="MHVAERSASWRCGERSAGPLLKMQWALADKLVYQKIREGTGGRL
                     RIVCSGGAPLSRALCEFFWSVGIPIYQGYGLTETSPIVSNNFPRNRPGSSGKPIPNCE
                     VRAADDGEILVRGPMVMQGYYKSPEATREVIDEDGWFHTGDIGYLDKDNYLFITDRKK
                     DLLKTSAGKFIAPQPIENSLKTSPYIANAMVVGDRRKFVVALVVPNCKTISAKAAEQG
                     VAISTGREVAADPFARTLIHAEIQRLTTHLAQFETIKRFALLPGDFTFDNGALTFTMK
                     LKRRVVEEQYHDVIESLYADVAEPRPVIQD"
BASE COUNT          573 a          745 c          859 g          568 t
ORIGIN      
        1 aaaaagcagg cactgcacga catgatcgcc agttgcctgg tgctacgctc gtgaacagta
       61 tccatgtcgc gactgtgggg tacgaatttt gcttacgaaa tttgatgcaa aaaggagaag
      121 caacgtgtct gaactcgccg cggatcaagc ttacgtcctg gtacaaaacg ctctgttagg
      181 aacgatcaaa agtgaaagcc gtaccaccaa gtcggttctg gcggcggttc cgaactcgaa
      241 tctggattac cgtcccgatt cctgcgccaa aaccgccatc gaactggtgc gccacattgc
      301 cgtgtccgag aatcgttttc tcgaatgcgt gagcaccggc atctacgaca caacgtcccc
      361 ggctcttccc gccaacatca ccgccccggc ggatattgcg gcctggtacg agcaaagttt
      421 tgcgcgaaat ttcgatgccg tgtcgaagct gtcgggcgaa cagctcatca agatggtgaa
      481 tttccgcgga atgttcgagc gcccggcgta tttcttcctg caactctgtc tggtgcactc
      541 cgtccatcac cgcggccagc tttccacata cctgcgaccc atggggagca aagtgccagc
      601 catctacggc gaaagctacg attcggcgga agcgaagaag gcggccgccg cgcaggcgca
      661 ggcctgatcc agcatattcg cagaggaaag gaccggccgc ggaagcgccc aggggttgtg
      721 atggagcggc gctgtttgtt cgacaccaac acgatcgacg gacaacggat cttcgagtgc
      781 cgcggcaacg aaatcgtgtt acgcgagaag tctggcgcag cctgcagcgg cgatccagca
      841 gttccaacaa atcacatcgt tcctggtagt gcttcccctt cctgagtcgt tgattcttgt
      901 gaccaacaac gagccagagt ttcggcggat caggggccta aggatggaaa actgggccgc
      961 gtaggcgagc aacgccagat tgattttcag cggacgacgc cggcaatcgg ggacgaggcg
     1021 gaggcgtaga gtctcttagg catgcggccg gcgagatagg ctttgcggcc cgcgctggtg
     1081 gcgtcgcgca tggcttcggc catgagctcg gcatcattgg cttcggcgat ggcggtgttc
     1141 atcagaattc cgtcggcgcc tagttccatc gccaccgccg cgtcggaggc agtccccacg
     1201 ccggcgtcca cgatcaatgg cacttccgtg atgcgttcgc gcaaaatccg gagtgcggcg
     1261 ggattttgca ctccgagtcc ggagccgatg ggcgcggcaa gcggcattac ggctgcagcg
     1321 ccggcgtcaa tcaatttgcg ggcggcgatc aaatcgtcgt tcgtgtaagg gagaacgacg
     1381 aagccttcct tgacgagaat ctttgtggct tcgatcagct gctcggtgtc ggggtagagc
     1441 gtttgctgat cgccgatgac ttcgagcttg acccaatttg acaggccgac ttcctgcgca
     1501 agacgcgcgg tgcggaccgc ttcatcggcg gtatagcagc cggcggtgtt agggagaatg
     1561 aatattttgt cgcgcggaat gaaatcgagc agcgattcct tgctcttgtc ggtcaggttt
     1621 acgcggcgaa cagcgacggt gaccatgtcc gcaccggaag cggcgtgcgc gcgggccatt
     1681 tcctgaaaac tgcggtattt cccggtgccg acaatcagcc gcgaggaaaa agcgcggccg
     1741 gcgataacga gctggtcaga catatcgatt cctcatggaa attgtagaac gtcggcttgc
     1801 acaggacaaa tttcgatctt cggttcagtc ctggataacg gggcgaggtt cggcgacgtc
     1861 ggcgtaaagg gattcgatga cgtcgtgata ctgctcttcg accacgcggc gcttgagttt
     1921 catggtaaag gtgagagctc cattgtcgaa ggtgaagtca ccggggagca acgcaaagcg
     1981 cttgatcgtt tcgaattgcg cgagatgggt ggtgaggcgc tgaatttcgg cgtgaatcaa
     2041 agtgcgagcg aaaggatcag ccgcgacttc tcgaccggtg gagatggcaa ctccctgctc
     2101 ggcagctttc gcggaaatgg tcttgcagtt agggacgacc agagcgacaa caaatttgcg
     2161 gcggtcgcca accaccatgg cgttggcaat gtacgggctc gtcttcagcg agttttcgat
     2221 gggttgcgga gcgataaatt tgccggcgga agtcttgagc agatctttct tgcgatcggt
     2281 gatgaaaaga tagttgtcct tgtcgagata gccgatgtcg ccggtgtgga accagccgtc
     2341 ttcgtcaatg acttcgcgag tggcttcagg cgatttgtag tatccctgca tgaccatcgg
     2401 cccccgaacg aggatttcgc cgtcgtcggc tgccctcact tcgcagttgg ggatgggctt
     2461 accggagctg cccgggcggt tgcgcgggaa attattggag acgatcggcg aagtctccgt
     2521 taggccgtag ccttgataga tggggatgcc gacgctccag aaaaattcgc agagcgcgcg
     2581 cgagagcggc gctccgccgg aacagacgat gcgcaggcga ccaccggtgc cttcacgaat
     2641 cttctggtag acgagcttat cggctaaggc ccactgcatt ttgagcagcg gtccggccga
     2701 tcgttcgccg caacgccagg atgcggagcg ttcggcgacg tgcat
//
LOCUS       DASTPM010000143         5489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_783192, whole genome shotgun sequence.
ACCESSION   DASTPM010000143 DASTPM010000000
VERSION     DASTPM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783192"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..2065)
                     /locus_tag="VFR42_02555"
     CDS             complement(23..2065)
                     /locus_tag="VFR42_02555"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEU0368062.1"
                     /translation="MPRRQFVSTAASAAALSTLSFGGRSLFANQSPAAAPAGTPWNEQ
                     GILHLTHSPYAKLKNVPVHAVTIQPGFWASRRTANVERSIPSMGKLLEVNGRMDNFLR
                     LIGKSDAPQRGPVYSDSDVYKWLEAVGFALQTESRPEMRAQADSIIKEVVAAQQPDGY
                     LNTYYVREHADQRMTAKTQQWGHELYNMGHMLQGATAYYRATGDQTMLNSGTRFVNEF
                     LLPTYGPEPNKKPLMSGHPEMEMALVELYRMTGDKRQLALAEYLLRGDERIPLKKNDY
                     VYHFCGKPFTSRTHLEGHAVRAMYACCGATDYYLETGDASYWKTLAVLWDDLISSQMY
                     ITGGVGARSDGEAFGNSYELPNSQAYGESCAAIGNMMWNWRMLAVTGDARYADVIERA
                     LYNGINSGMSLSGTLYCYRNPLSFDPATDEKIRNDWYDTTCCPPNLERTFASLPGYFY
                     STSDDGLYVHLFHNSELNWRLESGAPIKVTQRTEYPWKGTVEIEVSPASPQEFTLYLR
                     LPRWSNTPATTVNGKQAASALPGRYLPIRRRWVAGDLVKLQLDMTPQIMEANSRVVEN
                     NGRAAVQRGPLVYCLEGLDQKSGVSLHDLALTAFRTAGPGGFTDTFEKDLLDGVVVLQ
                     HEGAAIQTSESRRALYFSANTTPNKSSKISLTFIPYYAWNNRQPTEMLVWTRLLRS"
     gene            complement(2172..2837)
                     /locus_tag="VFR42_02560"
     CDS             complement(2172..2837)
                     /locus_tag="VFR42_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368063.1"
                     /translation="MAKTKQQIRILMADDHAIFRDGLRKLLDGDEEIVIVGEAQNGAE
                     CVKMLAKLKPDILLLDLRMPDKDGLAVLEEVNFDQLPTRVIVLTAAEDDRDVVRAMRL
                     GARGVVLKQSATDLLVKSIQRVHAGEIWLDNRMTAEVMKAFSKSSESGPRREKPLLSD
                     REKEIVQLVAQGFRNKEIGEKLFISEQTVKNHLHNIFDKLGVSDRLELALYAIHHRLI
                     DHT"
     gene            complement(2883..4562)
                     /locus_tag="VFR42_02565"
     CDS             complement(2883..4562)
                     /locus_tag="VFR42_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU0368064.1"
                     /translation="MSSHETDRRHFERQILYARPITILLSVLAILEWPATRQSNRCLY
                     FLFGYLLLSLAIMGLEVLLSEREWHSPLTLDLILLGVLIYLAPFTVPVWFPFLFVSYA
                     AGSRWGLSTAVPVTALLALIITLVDVVDRDTGGYHLITWIAIVGAMFASGAGLAFLGD
                     RSQKFAAQNHFLSSITATMQVDQGLAESLRLFLEELAVWFRAEQAFLVFRDMDLERIF
                     LWRLKTGDSERLTPHSFALARADGFLLDDLDATICWNSLQGAGSGFGWDRRDHEAIKS
                     LPRLPGPTQAEFRIKSFMTVTFDQNDKPAGRLFVVNGRRSNGPFSKADLDWLERVAKH
                     VVPSLENVFLLRHLRARAIEAERSRISRDLHDGILQTLLSIEIQLDVLRRRVGTAPDH
                     AEASLASLQRTVRNESAELRTFVTDLKPVRVQSADLVDLMRGFAERFRNESDLALDLL
                     VDSAELRAPDRVCREIFQIYREALNNIKKHAKATHVVVKLSQDDSRLLLVVDDNGEGF
                     SFAGKFTGDELDRLRLGPISIKERTRTVNGDLTVESNPGHGARLTIEIPLG"
     gene            5045..5356
                     /locus_tag="VFR42_02570"
     CDS             5045..5356
                     /locus_tag="VFR42_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368065.1"
                     /translation="MGAATPPDLLVNLMKSLVPIPLESAEKLKKEKQTRLGYQFISIK
                     LKDGRYFEQAVASEGCIIQIKGQKDIPFTEADVESVELSDRPWNFRRPVRVNRKTDVA
                     Q"
BASE COUNT         1216 a         1639 c         1493 g         1141 t
ORIGIN      
        1 gacgcataac caacgatcgc gatcaagatc gcagcaagcg cgtccacacc agcatttcgg
       61 tgggctggcg gttgttccag gcatagtaag gaataaaggt cagtgaaatt ttgctcgact
      121 tgttgggcgt agtgtttgcc gaaaaataaa gcgcacgccg agactcggac gtttgtatgg
      181 ccgcgccttc gtgttgcagc acgacgactc cgtcaagaag atccttttcg aaagtatcag
      241 tgaaaccgcc ggggccagcc gttcgaaaag cagttagggc caggtcatgc aacgaaaccc
      301 cagatttctg gtccagccct tccagacaat aaaccagggg gccacgctgc acagcggcgc
      361 ggccattgtt ctccacgacg cgcgaattgg cttccatgat ttgcggcgtc atgtcgagtt
      421 gcagcttcac gagatcgcca gcaacccagc gccggcgaat cggcaggtaa cggccgggga
      481 gtgcgctggc cgcttgcttt ccattcacag tcgtcgccgg agtgttcgac cagcgcggca
      541 agcggaggta cagcgtaaac tcctgcggcg acgcggggct gacttcaatt tcgaccgttc
      601 ccttccaggg atattccgtt cgttgcgtaa ctttgatcgg cgcaccgctt tccaggcgcc
      661 aattgagctc ggaattgtga aacagatgca cgtacagccc gtcgtcgctg gtgctgtaga
      721 aatatccggg cagcgacgca aaggtgcgct ccagattggg cgggcagcaa gtcgtgtcgt
      781 accagtcgtt gcgaattttc tcgtcggtcg cgggatcgaa cgacagcgga ttgcggtagc
      841 aatatagcgt tccgctcagc gacatgccgg aattgatgcc gttgtagagc gcgcgctcga
      901 ttacatcggc gtagcgcgca tcccccgtca ccgccagcat gcgccagttc cacatcatgt
      961 tgccgatggc cgcgcagctt tcgccgtacg cctgcgaatt cggcaactca taggaattgc
     1021 cgaatgcctc gccgtcgctg cgcgctccca cgccgccggt gatgtacatc tgcgacgaaa
     1081 tcaggtcgtc ccacaacacg gcaagcgtct tccagtaaga agcgtcgccc gtttccaggt
     1141 agtagtccgt cgcgccgcaa cacgcgtaca tggcgcgcac cgcgtgccct tccagatgcg
     1201 ttcgagaagt aaacggcttg ccgcaaaagt gataaacgta gtcattcttc ttcagcggaa
     1261 tccgctcatc gccgcgcaac aaatattccg cgagcgccag ctgccgtttg tcgccggtca
     1321 tgcggtacag ctccaccagc gccatttcca tttccggatg gccggacatc aacggtttct
     1381 tgttaggctc cgggccatac gtcggcagca gaaactcgtt cacgaaacgc gtgccggagt
     1441 tgagcatggt ctgatcgcca gtcgcgcggt aataggccgt cgcgccctgc agcatgtggc
     1501 ccatgttgta cagctcgtgt ccccactgct gggttttggc cgtcatgcgc tggtcggcgt
     1561 gctctctcac gtaataggtg ttcaaataac cgtccggctg ctgcgccgca acgacttcct
     1621 tgatgatcga atccgcctga gcgcgcattt ctgggcggct ctccgtttgc agcgcgaacc
     1681 ccaccgcttc cagccatttg taaacatccg aatccgagta aaccggcccg cgctgcggcg
     1741 cgtcgctctt gccaatcagc cggaggaaat tgtccatgcg cccgttcact tcgagcagct
     1801 tgcccatgga aggaatgctg cgctcgacgt ttgcggttct gcgcgacgcc caaaatccag
     1861 gctgaattgt cactgcatga acggggacgt ttttcagctt cgcgtaagga gagtgcgtca
     1921 gatgcaaaat cccttgttcg ttccaaggcg tgccggcagg tgctgcagca ggcgattggt
     1981 tggcgaatag cgaccgtccg ccgaacgaca acgtggacaa agccgcggcg gaggctgcgg
     2041 tcgaaacaaa ctggcggcgt ggcatgcgac gagacatatc cagcgctcct tggacatcaa
     2101 gcggcgaaag tttagctgga agcgcccgga aacgcgatag gcgtccgcca aatgaaaatg
     2161 tgcgatgaag cttaggtgtg atcgatcaag cggtgatgga tggcgtaaag agcaagctcc
     2221 aagcgatcgg aaacgccgag cttgtcgaag atgttgtgca ggtggttctt cacagtttgt
     2281 tcggaaataa acagtttttc accgatttcc ttgttgcgga atccctgcgc caccagctgc
     2341 acaatctctt tctcgcgatc ggagagcagc ggcttttccc gccgcggacc cgactccgac
     2401 gattttgaaa atgccttcat cacctcggcg gtcatccggt tgtccagcca gatttcgccg
     2461 gcatgcacgc gctgaatact tttgaccaac aggtccgtag cggattgctt gagcaccacg
     2521 ccgcgcgcgc ccaaccgcat cgcgcgcacc acatcgcgat cgtcttccgc cgcggtcaac
     2581 acgatcacgc gggtcggcag ttgatcgaaa ttcacttctt ccagcaccgc cagaccatcc
     2641 ttgtccggca tgcgcaaatc gagcagcagg atatccggtt tcaatttcgc cagcattttc
     2701 acgcattcgg cgccgttttg cgcttcgccg acgatcacga tctcttcatc gccatccagc
     2761 aatttgcgca agccgtcgcg gaaaatcgcg tgatcatccg ccatcaaaat ccgaatctgt
     2821 tgttttgtct tcgccataat aaactcaggg cactcgctca gccccgaaac tcgttccagc
     2881 catcatccga gcggaatctc tatcgtcaga cgtgccccat gtcccgggtt cgactccacg
     2941 gtcaaatccc cgttgacggt acgcgttcgc tccttgatgg agatgggacc cagccgcaga
     3001 cggtccaatt cgtcaccggt gaatttcccg gcgaagctga agccttcacc gttgtcgtcc
     3061 accaccaaaa gcaggcggct gtcatcctgc gaaagtttta ccacgacatg ggtggcctta
     3121 gcatgctttt ttatgttgtt gagtgcttca cggtaaatct ggaaaatctc gcggcagacc
     3181 cggtccggag cgcgtaattc cgctgaatcc accagtaaat ccagcgccaa atcagactcg
     3241 ttgcggaacc gctcggcaaa gccgcgcatc aggtccacca ggtcggcgga ctgtacccgc
     3301 accggcttca ggtccgtcac gaaggttcgc aactccgccg attcgtttct taccgtccgc
     3361 tgcaagctgg ccagcgaagc ttccgcatgg tcgggcgctg ttccgacccg ccggcgcagc
     3421 acatccagtt ggatttcgat gctcagcagc gtctgcagga tcccgtcgtg caggtcgcgc
     3481 gagatgcgcg aacgctcagc ttcaatggcg cgagctctca ggtgccgcag cagaaacaca
     3541 ttttcgagcg aaggcaccac gtgcttggct acccgctcca gccagtccag gtccgccttg
     3601 ctgaacggcc cgttgctccg cctgccgttg accacgaaca accgccctgc gggtttgtcg
     3661 ttctgatcga acgtcaccgt catgaacgac ttgatgcgaa actcggcctg tgtgggtccc
     3721 ggcagccgcg gaagactctt gatggcttcg tggtcccgcc ggtcccatcc gaatcccgat
     3781 cccgctccct gcaacgaatt ccagcaaatg gtggcgtcca ggtcgtccag caaaaagcca
     3841 tccgctcggg ccagcgcaaa actgtgtggc gtcagccgct cgctatcccc tgttttcaat
     3901 cgccataaga agatacgctc cagatccata tcgcgaaaca ccaggaatgc ttgttccgcg
     3961 cgaaaccaca cggccaactc ctccaggaac agccgaagcg actccgccaa accttgatcc
     4021 acctgcattg tcgctgtaat cgaagagaga aaatgatttt gtgcggcgaa tttctggctg
     4081 cgatcaccca ggaacgccag ccccgccccc gacgcaaaca tcgctcctac gatcgcgatc
     4141 caggtgatca ggtgatagcc tccggtgtcc cggtccacca catccaccag ggtaatgatc
     4201 aacgccagca gcgccgttac gggaaccgcc gtgctcaagc cccagcggct gccggcggca
     4261 taagaaacaa acagaaaggg aaaccagacc ggcactgtaa aaggtgcaag gtaaatcaga
     4321 actcccagca aaatcagatc cagggtcagc ggagaatgcc attcccgttc gctgagcaac
     4381 acctccaatc ccataatcgc caggctcagc agcagatagc cgaacagaaa gtagaggcac
     4441 cgattcgatt gcctggtagc aggccattcc aaaatcgcca gaaccgaaag cagaatggtg
     4501 atcggccgcg catacaggat ttgccgctca aagtggcggc ggtcggtttc gtgcgaactc
     4561 attgcgggct catgcgcggg gcaccgtgct agagccagtc taacaagcgg cttttgcgcc
     4621 taaatcaggg actgtgagtg ccgtttccaa gctgccaaga gttagcaaaa ggacaaattt
     4681 cgtgtccaaa ggttaatgcc agcatcgtcg gctattccgg caaattttcc ggcgccggcg
     4741 cggtcgactc tgaaaggcga accgttctca gacgtagctc agctggtgga gcgtcggtcc
     4801 gtggcttgta ggctgtacac acctaacacg ctgattcggt accgttagct cgcaaatcga
     4861 atttcttaaa ttacccccta gttttgccct cttgacccaa gaaacgccaa taccgaaaac
     4921 cgattttcgc ccgcagtgtc cagtccacaa aaggccgatg gttaaacctt tccgaccact
     4981 aggtttcgcg tttatcgcga cctgtcgtta atatgatatt cacagattta ggtccagcat
     5041 tgccttggga gcagcaacgc cgcccgactt attggtgaat ctgatgaaat ccttggttcc
     5101 cattcctctt gagagcgccg agaagttgaa aaaagaaaag caaacgcggc tgggttacca
     5161 attcatctca atcaaactca aagacggtcg atactttgaa caggcagtcg caagtgaggg
     5221 ctgtatcatc caaatcaagg gtcagaagga catccccttc acagaggccg atgtagaatc
     5281 agtcgagctt agcgataggc cgtggaattt caggcgcccc gttcgcgtga accgaaaaac
     5341 cgacgttgcg cagtaagaga ttggcttctt caaacttttt agagggtgaa gttgcgcgga
     5401 ggaaacggaa aaggattaca gcccgcaggt cggttcggag aatggtggaa agccgtagag
     5461 catcctgcag tcttcgcaga gagcgatgc
//
LOCUS       DASTPM010000144         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1158011, whole genome shotgun sequence.
ACCESSION   DASTPM010000144 DASTPM010000000
VERSION     DASTPM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158011"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1353
                     /gene="deoC"
                     /locus_tag="VFR42_02575"
     CDS             289..1353
                     /gene="deoC"
                     /locus_tag="VFR42_02575"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU0368066.1"
                     /translation="MTPPENPPRLEIVNRDGSAIAPVQSVSGQVAIAALSGHPLHPNR
                     GTSLNLDWVESVRVNTSAVERRAGTLVTRRTVKKDWQIAWLLRAITCMDLTTLSGDDT
                     DERVRRLCAKARQPVQQELLDRLGVRDLHIKVGAVCVYHLFAETAVKALQGSGIPVAA
                     VATGFPAGLSPLEERIAEIKESVEAGAQEIDVVITRAHVFGAQWQKLYDEIVAFKDAC
                     GPAHLKVILGTGDLLTLRNVARASLVAMMAGADFIKTSTGKETVNATLPVGLVMVRAI
                     REYAQETGMAVGFKPAGGIRTAKQSLEWLALMKEELGNSWLQPHLFRFGASGMLADIE
                     RQLEFHSTGRYSADHRHPLA"
     gene            1399..1854
                     /locus_tag="VFR42_02580"
     CDS             1399..1854
                     /locus_tag="VFR42_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368067.1"
                     /translation="MSKKASSTDAQLILQLYDLRREPEMRKARHWWMNEFWPGNADDF
                     LKVAWAMGSEENNWLRQVGGYWGLAASFVLQGALNAELFLRPSCSGEMFVMFAKVHPF
                     LKDLREKLGDPEAFLDIEKVITNTKWGRDRLKFILKRVESWREKRANQV"
     gene            1901..>3566
                     /locus_tag="VFR42_02585"
     CDS             1901..>3566
                     /locus_tag="VFR42_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0368068.1"
                     /translation="MSVIEKFTTMEYGPAPEDPKESLLWLDRHQRRFGHFINGKFQPP
                     SEGKYFETSDPGTGEKIADVAQGSAADVDAAVKSARAALPAWQKLSGHQRAKFLYALA
                     RQVQKHSRRLSVLETLDNGKPIRESRDIDIPLVARHFYHHAGWAQLLAQEFPGYQPCG
                     VVGQIIPWNFPLLMLAWKIAPALATGNTVVLKPAEYTPLTALAFAELTNEVGLPAGVV
                     NIVTGDGATGETLVKHPDADKIAFTGSTEVGRAIRRATAPSHKKLSLELGGKSPFIIF
                     EDADLDSAVEGLVDGIWFNQGQVCCAGSRLLVQESVAEKLTRKLQARMSTLRVGSPLD
                     KAIDIGAIIARVQLERIDGLVKQGIAEGATCWQPEIVLPSRGLYYKPTLLSNVHPTSI
                     VAQQEIFGPVLAAMTFRTPQEAVQLANNTAYGLAASVWSESVNMALQAAAQIKAGVVW
                     VNSTNLFDAACGFGGYRESGYGREGGREGLLEYLEPAWFKEAPTFAPNVAAELNPVFS
                     SSPSAVPDVANPITNIDRTVKLYIGGKQARPDSGYSVPVYGLKGELL"
BASE COUNT          751 a         1126 c          949 g          740 t
ORIGIN      
        1 gatggacgaa ctctacaaaa accttagcgc tggtcaggac cccgcatccg ctctccgtaa
       61 cgccaagctc accctggtcc attcccccgg aatctatcgc aagcccttct actgggctcc
      121 cttccttttg tattccggct cttaagtcct ttgtttgcta cacttgcgag aaagcgagcg
      181 gtaccccgcg ccctcgttcc aaaatggaac tgtgtcccga ctttcccgtt ctcgcctcct
      241 ctttgctcct ggtgttatcc tttctcgttc cgtggtactg gagttttcat gacgcctcct
      301 gaaaatcctc ctcgccttga aatcgtcaac cgcgacggct ctgctatcgc gccggtccag
      361 tctgtttccg ggcaggttgc catcgctgct ctctccggcc atccgctcca tccgaatcgc
      421 ggcacttctc tcaatctcga ttgggtcgaa tcggttcgcg tcaatacctc cgccgtcgag
      481 cgccgcgccg gcactctggt cacccgccgc acggtgaaaa aagattggca gatcgcctgg
      541 cttctccgcg ccatcacttg catggacctg accacgctct ccggcgacga caccgatgaa
      601 cgcgttcgcc gcctctgcgc caaagcgcgc cagcccgttc aacaggaact cctcgacaga
      661 ctcggtgtcc gcgatctgca catcaaagtg ggagccgtat gcgtctatca cttgttcgca
      721 gaaaccgcgg tgaaagcatt acagggcagc ggcattcccg ttgccgccgt ggcgacaggt
      781 tttcccgctg gtctttcgcc gctggaagag cgcatcgcag aaatcaagga atccgtcgaa
      841 gctggcgcgc aggaaattga tgtcgtcata acccgcgctc atgtcttcgg cgcccaatgg
      901 caaaagctct acgacgaaat cgtggccttt aaggacgcct gcggaccggc gcacttgaaa
      961 gtaattctgg gcaccggtga tttactcact ttgcggaacg tcgccagggc cagcctcgtg
     1021 gccatgatgg ccggcgccga cttcatcaaa acgtccaccg gcaaagagac cgtcaacgcc
     1081 actcttcccg tcggcctggt tatggttcgc gccatccgcg aatacgcaca ggaaaccggc
     1141 atggccgtcg gcttcaaacc cgccggcggc atccgcactg ccaaacaatc tctggaatgg
     1201 ctcgcgctca tgaaagaaga actcggcaat agctggctac agccccatct attccgcttt
     1261 ggtgccagcg gcatgcttgc cgacatcgag cgtcaactcg aattccactc gaccggccgc
     1321 tactcggcag atcatcgcca tccgcttgct tagccggcga ccatatttgg tgtagctcag
     1381 tctaaacgag gagagaagat gtcgaaaaag gccagctcca ccgacgcgca actcatcctt
     1441 cagctttatg acctgcgccg tgagccggaa atgcgaaagg cacgccactg gtggatgaac
     1501 gaattttggc cgggcaacgc tgacgatttc ctgaaagtcg cctgggccat gggctcagaa
     1561 gaaaacaatt ggttgcgtca agtcggcggc tactggggtc tggccgcctc attcgtcctg
     1621 caaggcgctt taaatgcaga gctgttcctt cggccctcgt gcagcggcga gatgtttgtg
     1681 atgttcgcga aggttcatcc ctttctcaaa gatctgcgcg agaagcttgg cgatcccgaa
     1741 gcatttctgg acattgagaa ggtgattacc aataccaagt ggggtcgcga ccggctcaaa
     1801 ttcattttga agcgcgtcga aagctggcgc gaaaaaaggg ctaaccaggt ctgaagcagc
     1861 accgaacttt cagcgcgtcg ccgcaggggc acgaagaaaa atgagcgtca tcgaaaagtt
     1921 caccaccatg gaatacggcc cggcgccgga agatccaaag gaatctttgt tgtggctgga
     1981 tcgccaccag cgacgctttg gccatttcat caacggaaaa ttccagccgc ccagcgaggg
     2041 aaaatatttc gagactagcg atcctggaac cggagaaaaa attgccgatg tcgcgcaggg
     2101 aagcgccgcc gatgttgacg ccgcggtaaa atcggcacgc gccgctctac cggcttggca
     2161 aaagctatcc ggacaccaac gcgcgaaatt cctgtacgcg cttgcgcgtc aggtgcaaaa
     2221 gcattcgcgc cgtctctctg tcctcgagac gctggacaac ggcaaaccaa tccgagagag
     2281 tcgcgacatt gatattccgc tcgttgcccg ccacttctat caccacgccg gctgggcaca
     2341 actgctcgcg caggaatttc ccggttacca accttgcggc gtcgtcggcc aaatcattcc
     2401 gtggaatttt cctctgctga tgctggcatg gaaaatcgct ccggcgctcg ccacgggaaa
     2461 tactgtcgta ctcaagcccg cggagtacac gccgctcacc gcgctcgcct tcgccgaact
     2521 cactaacgaa gtcggccttc ctgcgggcgt cgtgaatatc gtcacgggcg acggggccac
     2581 cggcgaaacg cttgtcaagc atccggacgc agacaagatc gctttcactg gctctaccga
     2641 agtcggccgc gccatccgcc gtgccacggc gccatctcac aaaaagcttt ctctcgagct
     2701 cggcggcaag tcgccgttca ttatttttga agacgccgat ctcgactccg ctgtcgaagg
     2761 attggtcgac ggcatctggt tcaatcaggg ccaggtctgc tgcgccggtt ctcgtttgct
     2821 ggttcaggaa tccgtcgccg agaaacttac ccgcaaactg caggcacgca tgagtacgct
     2881 ccgcgtcgga tcgccgctcg acaaagccat cgacatcggc gcgatcatcg cgcgtgttca
     2941 gttggagcgc attgacggcc tcgtcaaaca gggaatcgcc gaaggtgcca cctgctggca
     3001 gcccgaaatc gttctcccgt cgcgcggcct ctattacaag ccgacgctgc tgagcaacgt
     3061 tcatcccacg tccatcgttg cccaacagga aattttcgga ccagttctcg ccgccatgac
     3121 ttttcgcacg ccgcaagaag ctgtccagct tgccaataac acggcgtatg gccttgccgc
     3181 ctccgtctgg agcgaaagtg tgaacatggc gctgcaggcc gctgcgcaaa ttaaagccgg
     3241 cgtggtgtgg gtgaacagca ccaacctttt tgacgccgcc tgcggcttcg gtggctatcg
     3301 cgaaagcggc tatggtcgcg aaggaggccg cgaaggcctg ctcgaatatc tggaacccgc
     3361 ttggttcaaa gaagctccca ccttcgcgcc aaatgtagcg gcggaactta acccggtatt
     3421 ttcgtcttcc ccttcggctg tgcccgatgt agccaatccc atcaccaaca tcgatcgcac
     3481 tgtaaaactc tacatcggcg gcaaacaagc ccgcccagat tcaggctaca gcgttccggt
     3541 ctacggattg aagggcgaac tcctcg
//
LOCUS       DASTPM010000145         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_148729, whole genome shotgun sequence.
ACCESSION   DASTPM010000145 DASTPM010000000
VERSION     DASTPM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148729"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VFR42_02590"
     CDS             <1..1055
                     /locus_tag="VFR42_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368069.1"
                     /translation="IPTAAPRAANPADHGLRQSRVPAVSRANDFEVWNEPNIDFWVGN
                     PKESTYYELYDHAAAAVKHVSARLRVGGPATAQAAWADRFLAHCKEKNIPVDFVSTHV
                     YGNDKAEDVFATHENIPRTQMVCRAVKKVHEQIAASAYPKMPLIWSEFNADYSNQPQV
                     TDSAFMGPWLADTIRQCDGLVDSMSYWTFSDVFEEQGVVKTPFYGGFGLLAEDSIPKA
                     AFNDFAMLHQLGDTRLDVQSESALLTRRKDGSLAIAVWTLFLPEETGSPKTVTLHFAG
                     ASKAKTAQFTVVDKEHGSPLPAWEKMGSPKNPTAAQIEELRKAGAMPATQSQKLTNGA
                     LTLTLPPHALALITVK"
     gene            complement(1062..>2152)
                     /locus_tag="VFR42_02595"
     CDS             complement(1062..>2152)
                     /locus_tag="VFR42_02595"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0368070.1"
                     /translation="GLARLTKITTFLKGAPRYHGAWSHFMNGSTNQSLPVFDMIDDAG
                     DLVETSFLMQGLLTARQYFQGPSAAEKALYQSISDLWNTVEWDWYRRSPQSDALYWHW
                     SPDFSWHINHRLTGFNEVMIVYLLAVASPTHAVPPELYYTGWAGESKAAREYRKDWSG
                     TEDGERYANGHTYDGIKLDVGVGSGGPLFFAHYSYMGFDPHVRDRYTNYFENNRNLAL
                     INLAYCQRNPKGHKGYGANNWGLTASDGSEEYGAHAPDPRDDDGTMTPTGALASFPYT
                     PEASMAALRHFYRDLGNPLWDVYGPRDAFNLDTNWYSPIYMGLNQAPITVMIENYRTG
                     LIWKMFMSNPEIKPMLDKIGFKVEPGGR"
BASE COUNT          473 a          636 c          608 g          435 t
ORIGIN      
        1 gcattcctac tgcagctcct cgtgcggcta atcctgctga ccacggtcta cgtcagtccc
       61 gcgtgccggc ggtgagtcga gcgaacgatt tcgaggtgtg gaacgagccg aatattgatt
      121 tctgggtcgg caatccgaag gaatcgactt attacgagct gtacgaccac gccgctgccg
      181 ccgtcaagca cgtcagcgcc cggctgcgcg tgggcggacc ggcgactgca caggccgcgt
      241 gggcagatcg ctttctggcg cactgcaaag aaaaaaatat cccggtcgat tttgtgtcca
      301 cgcacgttta cggcaacgac aaagccgaag acgtctttgc cacccacgaa aatattccgc
      361 gaacgcagat ggtgtgccgc gcggtgaaga aagtgcacga acagattgcg gcgtcggcgt
      421 atccgaagat gccgctgatc tggagcgaat tcaacgcgga ttacagcaat cagccgcagg
      481 tcaccgacag cgcgttcatg gggccgtggc tcgccgatac catccggcag tgcgacgggc
      541 tggtcgactc catgagttac tggacttttt ccgatgtgtt tgaggagcag ggagtggtaa
      601 agaccccgtt ctacggcgga ttcggcctgc tggcggagga cagcattccc aaggcagcat
      661 tcaacgattt cgccatgctg catcagttag gcgacacgcg attggatgtg cagtcagaat
      721 ctgcgctgct gacgcgccgc aaagacggtt cgctggccat cgctgtctgg actctcttct
      781 tgcccgagga aaccggctca cccaaaacag tgacgctgca ctttgccggc gcatcgaaag
      841 caaaaaccgc gcagttcacc gtcgtcgata aagaacatgg ttcgccgctg ccggcctggg
      901 aaaagatggg cagcccgaag aatcccacgg cagcgcaaat cgaagaacta cgcaaagccg
      961 gcgcgatgcc tgcaacgcaa tcgcaaaaat tgaccaacgg cgcgctgaca ctcacgttgc
     1021 cgccgcacgc gctggcgctg atcactgtga aataagaaag gtcagcgccc cccaggctcg
     1081 actttaaaac cgatcttatc cagcatcggc ttgatttccg gattggacat gaacattttc
     1141 caaatcaatc cggtccgata attttctatc attaccgtga tgggcgcctg gttgagcccc
     1201 atgtaaatcg gtgaatacca gttggtgtct aaattaaacg cgtcgcgcgg cccgtacaca
     1261 tcccacagcg gattccccaa gtcgcgataa aaatgtctca gcgccgccat cgaagcttcc
     1321 ggcgtatagg gaaatgacgc cagcgcgcca gtcggcgtca tggtgccgtc atcgtcgcgc
     1381 gggtccggag catgcgcgcc atattcctcg gagccatcgc tggcggtcag tccccagtta
     1441 ttcgcgccgt agcctttgtg gcccttcggg ttccgctggc aatatgcaag attgatcagc
     1501 gcgaggttgc gattgttctc gaaataattg gtgtaacggt cacgaacgtg tggatcgaat
     1561 cccatataag aatagtgcgc gaaaaatagc ggcccgccgc tgcccacgcc cacgtcgagc
     1621 ttgatcccat cataggtatg cccgttggcg tagcgctctc cgtcctcggt ccccgaccaa
     1681 tctttgcgat actcacgcgc cgccttcgac tcgccggccc atcccgtgta ataaagctcc
     1741 ggtggcactg catgggtcgg cgaagcaacc gccaacagat aaacgatcat gacttcattg
     1801 aagccggtca ggcggtggtt gatgtgccag gaaaaatcgg gcgaccagtg ccagtaaagc
     1861 gcgtcgcttt gcggagagcg ccggtaccaa tcccactcta ccgtgttcca caaatccgaa
     1921 atgctctggt acagcgcctt ttcggccgcg ctcggcccct gaaaatattg ccgcgcggtc
     1981 agcaggccct gcatcaggaa cgaagtttcg accaggtcgc ccgcgtcgtc gatcatgtca
     2041 aataccggca agctctggtt cgtgcttccg ttcatgaaat gcgaccaagc gccgtggtag
     2101 cggggcgcgc cctttagaaa tgtggtgatt ttggtcagcc gggccaaacc tt
//
LOCUS       DASTPM010000146         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1100478, whole genome shotgun sequence.
ACCESSION   DASTPM010000146 DASTPM010000000
VERSION     DASTPM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100478"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..976)
                     /gene="ccsA"
                     /locus_tag="VFR42_02600"
     CDS             complement(152..976)
                     /gene="ccsA"
                     /locus_tag="VFR42_02600"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU0368071.1"
                     /translation="MAETSVFWLRVAAAFYSLGLLHAILTLTRRREQIFRVAIGASAL
                     GAVFHFVSIVEDGLATGQCPITNFYQTLSMCAFLIVLLYLFVEWRYRLQSMSVFLFPL
                     VFVMSLVATFGNPVGSWPNPVVGGAWLTAHIALALLGYAALALMALASVLYLAQERAL
                     KSKKPRKSYPWLPALGVLDEFIAKSMGAGFALITLAVITASIWAFIEHKAAWIRQPGI
                     PIAFFTWGVYLVLVCLRTTVGWRGRKAALMTVIVLACSAATWVAHAQLGALLAGRQ"
     gene            1394..2188
                     /locus_tag="VFR42_02605"
     CDS             1394..2188
                     /locus_tag="VFR42_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368072.1"
                     /translation="MAHRSRFVCAIAFVLVNAFAGFGQVTPSQPYVAASPVSCFLTSW
                     ESSLWRPVMNFFKTLFEQVPAEPEQVLADQPAVPEEPCGVAPLDPITDPAAQHLEASS
                     GSDVVDVENMVPAAARALDRFQSKVEGLGGTIVLKSAYRPASYQKHLQNVWYKWMDEL
                     RNNNDRGCQALRAQVQDEFTRHRLIETQHPVAISDHTRGLAFDATVALPASARIGRRR
                     MTLDGVARLAGLMRPAIAADPVHFKFLGGVRSASVKTAALRRRANA"
BASE COUNT          534 a          624 c          760 g          456 t
ORIGIN      
        1 cggttgcagg tggaaaggat catggcctcg cggacgcccg actgcgcttt caattcgcct
       61 aacgccgcgg gcacagtctc ttcgcggaag gcaagcgatt cgcgcacttc gactggagcg
      121 gtcttgtggc ttacgccaac gatcgccagc ttcattggcg ccccgccaga agcgctccca
      181 attgcgcgtg ggcgacccag gtcgccgcag agcacgcgag cacgatcacc gtcatcagcg
      241 cggctttgcg accgcgccat ccgaccgtag tgcgaaggca gacgagcacg aggtacactc
      301 cccaagtgaa gaatgcaata ggaattcctg gctgcctgat ccaggcggcc ttatgctcga
      361 tgaatgccca gatgctggcg gtgatgacag ccagcgtaat gagcgcgaaa ccggcgccca
      421 tggatttggc gatgaattca tcgaggacgc caagcgcggg gagccagggg taagatttgc
      481 gcggcttctt gcttttgagg gcgcgttcct gcgccaaata gagcaccgag gcgagcgcca
      541 tcagggcaag cgcagcatag cctaagagcg caagggcgat gtgcgccgtg agccaggcgc
      601 cgccgacaac tgggttgggc catgatccga ccgggttgcc gaaggtggcg acaagcgaca
      661 tgacgaagac caagggaaaa agaaagacgc tcatgctttg cagtctgtaa cgccactcaa
      721 cgaacagata cagcaacacg ataagaaagg cgcacatgga gagcgtctgg tagaagttgg
      781 tgattggaca ctggccggta gcgagcccgt cctcaacgat ggagacgaaa tggaagaccg
      841 ctccgagggc tgacgcaccg atggctacgc ggaagatttg ttcgcggcgg cgggttaggg
      901 tgaggatggc gtgcaggagt cccagggaat agaacgcagc cgcgacgcgc agccaaaaaa
      961 cgctggtttc agccatgaac gtgggtacct ttagtgtaac cggaggagga ccgggctttg
     1021 gggctagtgt ccgattttgg gattaggcag tttagcgaca aatcgtctag taaaaaactg
     1081 cttacggggg gacagaagtg tggatggttc tagaatcgat acgcgctgtc accctgatac
     1141 aacgcccttg aggttattgc gaccgggtgc aacgagattt cgcgaccggg acactggttg
     1201 aaggacgatg acgacgcagt ggccgaaact atctaagttg ttgatattaa atgggaattt
     1261 cctattaaag gaccttgtat aagggcatcc gaagaaaacg gccgtgcgcc gtcggtaatt
     1321 gcaacttcga caatggtagg aacgtcaaac tcattgagtc ccaagtacca accataacca
     1381 caggagtcca atagtggccc atcgttcgcg attcgtgtgc gcgattgcgt ttgtgctcgt
     1441 caacgctttt gcaggatttg gacaggttac cccctctcag ccctacgttg ccgcttcgcc
     1501 ggtatcatgt ttcctcacat catgggagtc gtcgctgtgg cggccggtga tgaatttctt
     1561 caagactctg ttcgaacaag ttccggccga gccggagcaa gttttggccg atcaaccggc
     1621 agtgccggag gaaccgtgcg gtgtggctcc actcgatccc attaccgatc cggcggcgca
     1681 gcacctggaa gccagttcgg gaagcgatgt ggtggatgtg gagaatatgg tacctgcggc
     1741 agctcgcgca ttggatcggt tccaatcgaa ggtggaaggt ttgggaggga cgatcgtgct
     1801 gaagtcggca tatcgtccgg cttcatatca gaagcacctg cagaatgtct ggtacaagtg
     1861 gatggacgag cttcgtaaca acaacgaccg aggatgccag gctctgcggg cacaggtgca
     1921 agacgagttc acgagacacc gtctgatcga gacgcaacat ccagtggcga tttcggacca
     1981 cacgcgagga ctagcgttcg acgcaaccgt ggcgcttccc gccagtgcaa gaatagggcg
     2041 ccggcgaatg acgctggacg gggttgcgcg cctggcgggg ctgatgaggc cagcgattgc
     2101 ggcggacccg gtgcacttta agtttctggg cggagtgagg agcgcatcgg tgaagactgc
     2161 ggctctgcgg cgccgggcga acgcatagga aaaaacggct caggcgagtg attggtcctt
     2221 ggccatctgg tgaacccgcc ggggagatcc aaggcgttcg aggcccgctc cgcatcatag
     2281 gcgaaatcct atttggctcc tgcgaagcca ggtcaggtca gacatcctta ccccggctct
     2341 gcctagttct tttcgagtgc ggagtctggc tggc
//
LOCUS       DASTPM010000147         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_148743, whole genome shotgun sequence.
ACCESSION   DASTPM010000147 DASTPM010000000
VERSION     DASTPM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148743"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFR42_02610"
     CDS             complement(<1..253)
                     /locus_tag="VFR42_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368073.1"
                     /translation="MKPIRIGTRGSLLAKWQAEYVRKRIFQATGVETEIVIIKTTGDS
                     MQQAPFSQIGGKGVFIKELEQALLEEHIDLAVHSVKDIPT"
     gene            complement(250..1527)
                     /gene="hemA"
                     /locus_tag="VFR42_02615"
     CDS             complement(250..1527)
                     /gene="hemA"
                     /locus_tag="VFR42_02615"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU0368074.1"
                     /translation="MEIVLVGLNHRSAPVEVRERVSFTADQARQAAEELRARGIFHET
                     LVLSTCNRSEVYGVPPEASRESAAALSSYLSDFHAVQLEQLNGSLYRHYDRHAVRHLF
                     RVASGLDSMVLGEAEILGQVREAYRVAHESGATGPVLNRMFQGALEVGKRVRHETELG
                     ARPMSVASAGVKLAERIFGKLNQRTALVLGAGTISEQVVAQLRDRSIAHLYVMNRSRD
                     RAELLAREFSGKVINWEDWDAALAQPDVIVSSISTEEPALKHAMLERAMAARGNRPLF
                     VMDLGLPRNVESTAADLYNLYLYNIDDLTQIVEHNRGARQSQVPRAEAIVDEHVDKFL
                     TWQASVELIGMVETLRGKLKEERAAFVLERVQRVPNLSPEQREQMESLMDELLEKLLL
                     DPARRLQGEKDLRRKIQNVEAIRELFLSGRERA"
     gene            complement(1546..2358)
                     /gene="ccsA"
                     /locus_tag="VFR42_02620"
     CDS             complement(1546..2358)
                     /gene="ccsA"
                     /locus_tag="VFR42_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU0368075.1"
                     /translation="MDHLFFLLTLAAYLGSFACYAGYLVAGREWSGRAATLLLAAGLV
                     VHYYALLERSRGLHTVPYHDLAGSMSLFGWLLALTYLSLEVFHRQRSVGALVLPFVLA
                     FFLFANAATPDHNAAAPAKGVAFALHVTLSILAYAAFGLSCVLSVIYLAQQRLLQKHK
                     VGSVLWRLPPLDLLDRMSQSSVLVGLIAITIGTSLGFLWVDRLTGAVWHYDPKYVVTL
                     LVLAAYVIYLRLARNPAWRGSRASRLCIFNFAIVIFSFTVVNLFFSHAHRYF"
     gene            2505..3569
                     /gene="hemE"
                     /locus_tag="VFR42_02625"
     CDS             2505..3569
                     /gene="hemE"
                     /locus_tag="VFR42_02625"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU0368076.1"
                     /translation="MTSSSVAPTRKELFLRACRGEALPRVPVWMMRQAGRYLPEYREL
                     RARHPFLEVCKTPDLAVEVSLQPFRRLDVDAIIVFSDILIPAEAMGVPLELGDAGPHL
                     LEPVRSAEAVRKLHLFDPEADTGFLMEAIRRLVRAAGPDVPVLGFAGAPWTMACYMVE
                     GRTKEGFATVKSFLYAEPATFRELLHRIAQATVGYLTAQIAAGAAAVQLFDTWCGELA
                     LPEYREFALPAVQEIIAGLPGTVPVIYYSKASHHLLSAVADSRADVLSLDWRVDLGEL
                     RAKLGSRVALQGNLDPAVLFGPHENIVGATRRILGQLGGKGHILNLGHGILPATPIEA
                     AQAFIRTGQQFPFSSTAANS"
BASE COUNT          768 a         1086 c         1030 g          706 t
ORIGIN      
        1 cggtgggaat gtctttgaca ctgtgaacgg ccaaatcgat gtgttcttcg agaagagctt
       61 gttcaagctc cttgatgaag acgcctttgc cgccgatttg agaaaagggc gcctgctgca
      121 tgctgtcgcc cgtggtcttg atgatgacga tctcggtttc aacgcccgtc gcctggaaga
      181 tgcgcttgcg cacgtattcc gcctgccatt tggcgagaag gctgccccgg gttccgattc
      241 gaatgggctt cacgcgcgct cccggccgga cagaaaaagc tcacggatgg cttcgacgtt
      301 ctggattttg cgccgcaagt ctttttctcc ctgcaggcga cgggcgggat cgagcagcag
      361 cttttccagc aattcatcca tgagagattc catctgctcg cgctgctcgg gcgaaagatt
      421 cgggacgcgc tgcacacgtt caagcacaaa tgcagcgcgc tcctctttca gtttgccacg
      481 caacgtttcg accatgccta tcagttcgac gctggcttgc caggtaagaa atttgtccac
      541 atgctcgtcc acgatggctt ccgcgcgtgg cacttggctt tgccgcgcgc cgcgattgtg
      601 ctccacgatt tgcgtcaggt cgtcgatgtt gtaaaggtag aggttataga gatcggcagc
      661 ggtcgattca acattgcgcg gaaggcccag atccatcacg aagagcggac gatttccgcg
      721 ggccgccatg gccctctcca acatggcgtg cttgagggca ggctcttcag tggaaatgct
      781 ggacacgata acgtcgggct gagcaagcgc cgcatcccag tcctcccaat taatcacttt
      841 gcccgaaaat tcccgtgcca gcagctcggc ccgatcgcgc gaacgattca tcacataaag
      901 gtgcgcgatg ctgcggtctc gcaattgcgc gacgacttgc tcgctgatgg tgccggcacc
      961 aaggacgagc gcagtccgct ggttgagttt gccaaaaatt cgttcggcca gcttgactcc
     1021 cgcggaagca acggacattg ggcgagcgcc aagctccgtc tcgtggcgca cccgttttcc
     1081 tacctccagc gcaccctgaa acattcgatt cagaacgggg ccggtcgccc cggattcgtg
     1141 tgcaacacga taggcttccc gcacctggcc tagaatttca gcttcgccca gcaccatgga
     1201 gtccaggccg gacgccacgc gaaaaagatg gcgcacagca tggcggtcgt agtggcgata
     1261 aagcgagcca ttcagctgtt cgagctgcac cgcatgaaag tcgctgagat agctcgacag
     1321 tgctgctgct gactcgcgcg aggcttccgg aggcacgccg tacacttcac ttcgattgca
     1381 ggtggaaagg accagggtct cgtgaaaaat cccgcgcgcg cgaagttcct cggcggcctg
     1441 gcgcgcctgg tcggcggtga acgacacacg ctcgcgcacc tcaaccgggg ccgagcggtg
     1501 attcagtccg acgagcacaa tttccataac gctaattgca tgctcctaaa aatagcgatg
     1561 cgcatgcgag aaaaataggt tcaccactgt gaagctgaaa atcacgatag cgaagttaaa
     1621 aatgcaaagt cgcgaggccc gcgaaccgcg ccatgccgga ttgcgcgcca ggcgcagata
     1681 aatgacgtat gcggccagca ctaggagcgt aacaacatac ttgggatcgt agtgccagac
     1741 cgcacccgtc agccggtcca cccacaagaa acctagcgac gtcccaattg tgattgcaat
     1801 caagccaaca agtacgctgc tctgactcat ccggtcgaga agatcgagcg gcggaagccg
     1861 ccacagtacg cttcccactt tgtgtttttg cagcagccgc tgctgcgcca ggtaaataac
     1921 actgagcacg caggaaaggc caaacgcggc gtacgcgaga atgctgagcg tgacgtggag
     1981 cgcgaacgca actcctttgg cgggcgcagc ggcattatga tccggtgtgg ccgcattggc
     2041 aaaaagaaaa aacgccaaca cgaacggaag gaccagcgcc ccaacagaac gctggcggtg
     2101 gaaaacttcc aggctcagat aagtcagcgc gagcagccag ccaaacagcg acatcgaacc
     2161 ggccaagtcg tggtaaggga cagtgtgcaa cccgcgcgaa cgctccaaca aagcgtaata
     2221 gtgaaccacc agccctgcag ccagcagcag cgtagctgcc cgtccagacc attcccggcc
     2281 cgcaaccagg taccccgcat agcaggcaaa gctgcccaga tatgccgcta gtgtaaggag
     2341 gaaaaagaga tggtccatgg tcggctcgaa ctgcgcgcaa ttccggcgtg gctggtgctc
     2401 ggagtcctgg agagaagtat agcgtagcca ctacgcctcc gcatgcgcaa cgctgcgcag
     2461 agtaaacaac gttcctgccg atttgctatc ctgacggtgc ccacatgacc tcttccagtg
     2521 ttgcgccgac tcgaaaagaa ttgttcttgc gtgcgtgtcg tggcgaagct ctgccgcgcg
     2581 tgccggtctg gatgatgcgc caggcggggc gctatttgcc cgaataccgc gaactgcgcg
     2641 ccaggcaccc gttcctcgaa gtctgcaaga cacccgatct ggcggtcgag gtttctctcc
     2701 aaccttttcg ccgccttgat gtggacgcca tcatcgtctt ctccgatatc ttaattccgg
     2761 cggaggccat gggcgtcccg ctggaacttg gcgatgcggg gccgcatctg ttggaacctg
     2821 ttcgctcggc ggaagcggtc cgcaaactgc atctgttcga tcccgaagcc gatacgggct
     2881 tccttatgga ggcgattcgg cggctggttc gcgcggcggg gccagatgtg cccgttctcg
     2941 gctttgctgg cgccccctgg actatggctt gctacatggt cgagggccgc accaaagaag
     3001 gcttcgccac tgtgaagagt tttctctacg ccgagcccgc aaccttccgc gaactgctcc
     3061 accgcatcgc ccaagcgacg gtcggctatc taacggcaca aatcgcggca ggagccgcag
     3121 ccgtacagct gttcgacacc tggtgcggcg aactggcact gcctgaatac cgggaattcg
     3181 ccttgcctgc tgttcaggaa atcatcgctg gcttacccgg taccgtgccg gtcatttatt
     3241 acagcaaggc gtcgcaccat ttgctttccg cagtcgccga tagcagggcg gacgtgttga
     3301 gcctagactg gcgcgtggac cttggcgaac tgcgcgcgaa gctcggatcc cgcgtggctt
     3361 tacagggaaa tctcgatccc gccgttttgt tcgggccgca cgaaaatatc gtaggcgcaa
     3421 cgcggcgaat tctcggtcag ttgggcggca aaggccacat tctcaatctc ggtcatggca
     3481 ttctccccgc caccccaata gaagccgcgc aggcgtttat tcgaaccggc caacaatttc
     3541 cgttctcgtc taccgccgca aattcctgaa actacccgca tccacagaaa
//
LOCUS       DASTPM010000148         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_350930, whole genome shotgun sequence.
ACCESSION   DASTPM010000148 DASTPM010000000
VERSION     DASTPM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350930"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1196)
                     /gene="queA"
                     /locus_tag="VFR42_02630"
     CDS             complement(99..1196)
                     /gene="queA"
                     /locus_tag="VFR42_02630"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU0368077.1"
                     /translation="MQITELDYQLPQELIAQQPLAERDASRMLLLDRCSGSVQDDHFV
                     ALAERLRGNELLVFNNARVSPARLLGRKAGIHAQPPSHASAREHLTGTVEVFLSRQIE
                     PDTWEALVRPGRKLPAGERVIFGKGELEAEIVARGQFGLRTVRFISRDDKTPSVHIEQ
                     LGHVPLPPYIEREDESSDRERYQTVFAKRPGAVAAPTAGLHFTQQTMERLSGKGIQTC
                     EITLDVGLGTFQPIHSETLEGHKIHGEAYEIPQDTAEAVREARAAKKPLIAIGTTVVR
                     ALEDAAQRAVQENRKEPVLAGRSEARIFITPGHEFRVVDGMLTNFHLPRSTLLALVCA
                     FAGREKVLAAYQHAVAARYRFYSYGDCMLIR"
     gene            1436..1903
                     /locus_tag="VFR42_02635"
     CDS             1436..1903
                     /locus_tag="VFR42_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU0368078.1"
                     /translation="MATLDRWEPFRGSYDSQLNRLFTDFFGRASQDQNLTPWAPAVDI
                     FEGEHELVVKADLPDIKPEELDIRVENNILTIRGERKFEKKVDEKNYLRVERSYGSFA
                     RSFSLANTVNTEAIKADYKDGVLTLSIPKREEAKPKQIKVSVGGQASAAAAAR"
     gene            1997..>4571
                     /gene="clpB"
                     /locus_tag="VFR42_02640"
     CDS             1997..>4571
                     /gene="clpB"
                     /locus_tag="VFR42_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEU0368079.1"
                     /translation="MLRFEKLTVKAQEALQDANEVAARHENQEIAPLHLLRALVHQAE
                     GVVPPLLARLGIRKEALDSEVQREMGRLPRVHGFSEQRMGRALNDALERAFKEADNFK
                     DDYVSTEHLFLGIASLDRDPAGQLLKKLGASHEAILQALTGVRGTQRVTSQNPEATYE
                     ALEKYARDLTELARKSKLDPVIGRDEEIRRVMQILARRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIISGDVPDVLKTKRLVALDLAALVAGAKFRGEFEDRLKAVLKEISQAEGQIIL
                     FIDELHTLVGAGAAEGAMDASNMLKPALARGELRAIGATTLNEYKKYIEKDPALERRF
                     QPVLVSEPTVEDTIAILRGLKERYEVHHGVRIKDAAIIAAATLSHRYISDRFLPDKAI
                     DLIDEAAASLRMQIDSLPTEIDEIERRIMQLEIERQALLKESDAHSKERRAQIEKELA
                     ALKEDSSGRKARWQAEKHAIGRIRKLKEQIEQLKAEEQRYERSGELAKVAEIRYGRIA
                     AAEAELKKAQDDFAAMQKDAPMLKEEVDEEDIAKLVSKWTGIPAGRLMEGEAQKLVKM
                     EQRLRQRVVGQDDALGRVANAVRRSRAGLSDTKRPIGSFIFLGPTGVGKTELARALAE
                     FLFDDEKLMIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEQVRRHPYSVVLFDE
                     VEKAHPDVFNVLLQILEDGRLTDGKGRTVDFRNTVLVMTSNVGSSAIFELSAQNPERA
                     RKEAMEALRASFRPEFINRIDEIVIFNPLGKAQLEGIVKLLLKNVEKLLEERKITLEL
                     TPKAYELLLEEGYDPAYGARPLRRTIQRLIQDPLALQILEGKVLPGDHVRVDRDGKSG
                     AM"
BASE COUNT         1006 a         1402 c         1277 g          886 t
ORIGIN      
        1 gactcctctc agtgcgaaga aggaaatcgt cgctccgtgc ggcttccttc ttcgcagttt
       61 ttttctcttt gcgacctttt gacggcgggc atatcgcctc agcgaatcag catgcaatcc
      121 ccgtaactgt agaagcgata ccgcgccgcc actgcatgct ggtacgccgc cagcactttt
      181 tctcttccgg cgaacgcgca caccagcgcc agcaatgtcg atcgcggcag atgaaagttc
      241 gttagcattc cgtccactac gcgaaattcg tggccgggcg tgatgaaaat gcgcgcctcc
      301 gaacgccccg caagcaccgg ctctttccta ttctcttgca cagcgcgctg tgccgcatcc
      361 tccagcgcgc gaaccacggt agtgccgatc gcaatcagcg gttttttcgc ggcgcgcgcc
      421 tcgcgaacgg cttctgccgt gtcttgcgga atctcatacg cttcgccgtg aatcttgtgc
      481 ccttcgagcg tctcactatg aatgggctga aacgtgccca accccacgtc cagcgtaatt
      541 tcgcaagtct gaatcccttt gccgcttaaa cgctccatcg tctgctgcgt aaaatgcagc
      601 ccggccgtgg gcgccgcaac tgctcccggt cttttcgcga acacggtttg gtatctttcg
      661 cggtccgaac tttcatcttc tcgttcgatg tatggtggca gcggaacatg gcccagctgc
      721 tcgatatgca ccgacggtgt cttgtcatcg cggctgataa agcgcactgt ccgcaaaccg
      781 aactggccgc gtgctacgat ctccgcttcc agttcgcctt tgccaaaaat tacccgctcg
      841 ccagccggca gcttgcgccc cggccgcacc agcgcctccc aagtatccgg ttcgatctgg
      901 cggctcagaa acacttctac ggttcccgtc agatgctcgc gcgccgaagc atgcgaagga
      961 ggctgcgcgt gaatccctgc cttccggccc aagagccgtg ccggactgac ccgggcgttg
     1021 ttgaatacca gcaactcgtt tccgcggagc cgttcggcta acgccacaaa gtggtcgtcc
     1081 tgcacgctcc ccgagcatcg gtcgagcaaa agcatccggg aagcgtctcg ctccgccaga
     1141 ggctgctggg cgatcagttc ctgaggcagc tgatagtcga gttctgttat ctgcatgaac
     1201 gtaagtttac acgcctcccc aattccgaga aaatgcgtaa cttatccccg acaatggcgc
     1261 aataagggag gcaacagcct gcgtcgtcta aggctcttta cggactcggg gcgctgtaag
     1321 ctattcaata taggtatttt accggaaagg tggtggcaag ctaaaatata gttgacaata
     1381 gtatgctcat attttatgat ctgtgtgttg taagtttatt ggaggtgcct gttccatggc
     1441 aactttggat cgttgggaac cctttcgcgg ttcctatgac tctcaactga accgcttgtt
     1501 cactgacttt ttcggccgcg cgtcgcaaga ccagaacctg acgccgtggg ctccggcagt
     1561 ggatatcttc gaaggtgagc acgaactcgt cgtcaaggcc gacttgccgg acatcaagcc
     1621 ggaagagttg gacattcgcg tcgagaacaa cattctcacc atccgcggcg agcgcaagtt
     1681 cgagaagaaa gtggacgaaa agaactacct gcgcgtcgag cgcagctacg gctctttcgc
     1741 tcgcagcttc tcgctggcca acaccgtcaa caccgaagcc atcaaggccg actacaaaga
     1801 cggcgtgctg acgctgagca ttcccaagcg ggaagaggcc aagccgaagc agatcaaggt
     1861 gagcgtcggc ggtcaggcgt cggcggcggc agcggcgcga taagcgcgac ctgtcggttc
     1921 accaaagccg aagtcaggga gtacgatggg gctggccgcg cgccagccct ttttttgaag
     1981 cggggagtca attgaaatgc tgcgatttga aaaattaacg gtaaaagcgc aggaagcgct
     2041 gcaagacgcc aatgaagtcg ccgcgcgcca tgaaaatcag gaaattgcgc cgttgcatct
     2101 gcttcgagcg ctggtccatc aggcggaagg agtcgtccct ccgctgctcg cgcgtctcgg
     2161 catccgcaaa gaagcgctcg actccgaagt ccagcgcgaa atggggcgac tgccacgcgt
     2221 gcacggcttt tccgaacagc gcatgggccg cgcgttgaac gacgcgctcg agcgcgcttt
     2281 caaggaagct gacaatttca aggacgacta cgtttccacc gaacatctgt ttctcggcat
     2341 cgccagtttg gatcgcgacc ctgccggcca gcttctgaaa aaattgggag cttcgcacga
     2401 agccattctc caggcgctca ccggcgtgcg cggcacgcag cgtgtcacct cgcaaaatcc
     2461 ggaggctacc tacgaggctc tggagaagta cgcccgcgat ttaactgaac tcgctcgcaa
     2521 atccaagctc gacccagtca tcggccgcga cgaagagatt cgccgcgtca tgcagatcct
     2581 ggcgcgccgc accaaaaata atccggtgct gatcggcgaa cctggcgtcg gcaagacggc
     2641 tatcgtcgaa ggcctggccc agcgcatcat ttccggcgac gtgcccgacg tgctgaaaac
     2701 caagcgtctg gtcgcgctcg atcttgcagc cctggttgcc ggcgcaaaat tccgcggcga
     2761 atttgaagac cgcctcaagg ccgtcctgaa agaaatctcg caggctgaag gccagatcat
     2821 tctgttcatc gacgagctgc acaccttggt cggcgctggc gccgccgaag gcgccatgga
     2881 cgcttccaac atgctgaagc cggctctcgc tcgtggcgaa ctccgtgcga tcggcgccac
     2941 cacgctcaac gagtacaaaa aatacatcga aaaagatccc gcgctggagc gccgcttcca
     3001 gcccgttctg gtcagcgagc cgaccgtgga agacaccatc gccattttgc gcggcttgaa
     3061 ggaacgctac gaagtgcatc acggcgttcg catcaaagac gccgcgatta ttgcagcggc
     3121 cacgctttcg catcgctaca tttccgaccg ctttctgccg gacaaagcca tcgacttgat
     3181 tgacgaagcc gccgcgagcc tgcgcatgca aatcgattct ttgcccacgg aaatcgacga
     3241 aatcgagcgc cgcatcatgc agctcgaaat cgagcgccag gcgttgctta aggaatccga
     3301 cgcccactcg aaagagcgtc gcgcgcaaat cgaaaaagaa ctcgctgccc tgaaagaaga
     3361 ttccagcggc cggaaagcgc gctggcaagc tgaaaaacat gccatcggca gaatccgcaa
     3421 actcaaggag cagatcgagc agctcaaagc ggaagaacag cgctacgaga ggtccggcga
     3481 acttgccaag gtcgcggaaa ttcgctacgg ccgcatcgcc gcagccgaag ctgagctcaa
     3541 gaaagctcag gacgacttcg ccgcaatgca aaaagacgcg cccatgctca aggaagaagt
     3601 cgacgaagag gacatcgcaa aactggtcag caagtggacc ggcattcccg caggccgcct
     3661 tatggaaggc gaagcgcaaa agctcgtcaa gatggagcaa cgcctccgcc agcgcgtcgt
     3721 gggacaggac gatgctctcg gccgcgtcgc caatgctgtt cgccgcagtc gcgccggcct
     3781 ttccgacacc aagcgcccca tcgggtcatt tatttttttg ggaccgacgg gggtgggcaa
     3841 aactgaactc gcgcgcgccc ttgctgaatt tcttttcgac gacgaaaagc tcatgatccg
     3901 catcgacatg agcgagtaca tggaaaagca cagcgtctcg cggttgatcg gcgcgcctcc
     3961 gggctatgtc ggttacgaag aaggtggcca gctcaccgaa caggtgcgtc gtcatcccta
     4021 ttccgtggtg ctcttcgacg aagtcgaaaa agcgcatccc gacgttttca acgtgctgct
     4081 gcaaattctc gaggacggcc gcctcaccga cggtaagggc cgcaccgtgg atttccgcaa
     4141 caccgttctg gtcatgacaa gcaacgtcgg ctccagcgcg atttttgaac tgtccgcgca
     4201 aaatcccgag cgcgcccgca aggaagccat ggaagctctt cgcgcgtcgt ttcggccgga
     4261 gttcatcaac cgcatcgacg aaatcgttat cttcaacccg ctcggcaaag cgcagctcga
     4321 aggcatcgtc aagctgctgc tcaagaacgt cgaaaagctg ctcgaggaac gcaaaattac
     4381 gctggagctt acgccgaagg cgtacgaact cctgctcgag gagggctacg atcccgcgta
     4441 tggcgctcgt ccgctgcggc gcacgatcca gcggctcatt caggatccac tggctctgca
     4501 aattctcgag ggcaaggttc tccccggcga tcacgttcgc gtcgatcgcg acggcaagag
     4561 cggcgccatg c
//
LOCUS       DASTPM010000149         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_639345, whole genome shotgun sequence.
ACCESSION   DASTPM010000149 DASTPM010000000
VERSION     DASTPM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639345"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2529)
                     /locus_tag="VFR42_02645"
     CDS             complement(<1..2529)
                     /locus_tag="VFR42_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0368080.1"
                     /translation="MAKLFRVPLCLWAMFALCARAQEFRGSLLVEVRDASGGAIPSAK
                     AELTDAGSGLAQSVTANSLGEARFQAIPPSVYSLTVTAVGFAAQTEKITVAIGSRPTV
                     RVTLRPESVRHSVEVHDRGPSLASQPLETTSSTVQTLITANDIDEVPLSARSFANIAL
                     MAPFTAPVEPSDPTKARITAVSFGGSSGLNVDLSVDGGDNNDDFIGGFLQNYSPEAMQ
                     EFVVRSAQFGADTSRTNGGSIILSTRRGSNDWHGSASYYYRGKSLNARNPLDNPEPDP
                     KQPFARQNGVATIGGPLRRDKLWFFSSYEYVDENASVAYSANSQTEFRALGQLAADGL
                     IPAVPSIAVPTNVPVPCRDTLFMGRVDWAQSERSQWFLRGSLDRNHTQNDLVQQAALP
                     STGSTTTSNYFNILLGQQFRFSPNWTGALTLEASNFHHTKERNSNLGLALAFPFSANF
                     HTTSGFETFGDNQFATEISAFPVVRDQQKYQFRYDVFHTAGQHAVRFGLNLIHEPVLR
                     GALAASAEHLVQFTQDPSFYLSNPAQFVADYNCTAAALPDTTCNDTDAGDGTFAQSLR
                     RLGFYVEDSWHVTPSLTVNAGLRYDTTFGLFIASGRNQMQNPAFSTLQALNIPLATGI
                     PHDDRDALAPRLGIAYAPGGSQRTVLRAGIGLYYNDLAQNGWVDAFTAVNQTITPCTT
                     YDLTNPACLPSGEDGGQGALIDPHYHTPYALQASAGVEHDFGKSWRIGVTYEHQQGVH
                     QYRRYEYVAGFTLPAESPNISLFRSDNRSSYNGLAVQLQHRFSNRFELTANYVLASAT
                     TWGAVVGELFDYVNGVSNPLNAFGPGDHGPSGEDIRHRFVLIGTL"
     gene            complement(2613..>2931)
                     /locus_tag="VFR42_02650"
     CDS             complement(2613..>2931)
                     /locus_tag="VFR42_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0368081.1"
                     /translation="GQNSIDQWMIPYFGAPVYDDPAVYAKSSPITFIKNVKTPTLVIV
                     GDSDGECPTPQSYEFWHALKTLGVETQLVVYKNEGHHFVKPEHLRDRIRRTVAWFDAH
                     LQH"
BASE COUNT          625 a          879 c          827 g          600 t
ORIGIN      
        1 cagcgttccg atcagtacaa agcggtggcg gatgtcttct ccggaagggc cgtgatcgcc
       61 aggcccgaaa gcgttcaatg gattcgacac gccgttcaca taatcgaaca gctcgccgac
      121 aacagcgccc caagtcgtcg cgctggccaa aacataattg gctgtcagct cgaaacgatt
      181 cgaaaaacgg tgctgtagtt gcaccgcaag gccgttgtag ctggagcggt tgtcgctgcg
      241 gaacaatgaa atattgggtg actccgctgg tagcgtgaat cccgcaacgt attcgtagcg
      301 gcgatactgg tgtacgccct gctgatgctc gtaggtgact ccgatccgcc agcttttacc
      361 gaaatcatgc tccactccgg cgctggcctg cagcgcataa ggcgtgtggt agtgcggatc
      421 gatcagcgcg ccctgcccgc catcttctcc gcttggcaaa caagcgggat tcgtcaaatc
      481 gtaagtcgtg cacggcgtga tcgtttggtt gactgccgtg aacgcatcca cccaaccgtt
      541 ctgcgctagg tcgttgtaat acagtccgat gccggctcga agcaccgttc gctgcgaacc
      601 gcccggcgca taggcgatgc ccaaacgagg tgcgagcgcg tcgcgatcgt cgtgggggat
      661 tccagttgcg agcggaatat tcagagcttg cagcgtcgag aacgccggat tttgcatttg
      721 atttcggccc gacgcgataa acaacccgaa cgtcgtgtcg tagcgcagcc cggcattaac
      781 cgtcagcgac ggtgttacat gccaactgtc ttccacataa aagcccagcc gccgcaaact
      841 ttgcgcgaac gtgccatcac ccgcgtccgt atcgttgcac gtcgtatccg gcaaagccgc
      901 cgcggtgcag ttgtaatccg ccacaaactg cgccggattc gacaaataga aggacggatc
      961 ctgagtgaac tgcaccagat gttcggctga agccgccagc gcaccgcgca gcaccggctc
     1021 gtggatcaga ttcaacccga atcgcactgc gtgctgtcca gcggtgtgaa agacgtcgta
     1081 ccggaattga tacttctgct ggtcccgcac caccgggaac gccgaaattt ccgtcgcgaa
     1141 ttggttgtct ccaaacgttt cgaagcccga agtcgtatga aaatttgcgc tgaagggaaa
     1201 cgccagtgcc agccccagat tggagttgcg ttccttggtg tgatgaaaat tgctggcttc
     1261 cagcgtcagc gcgccggtcc agttgggtga gaagcggaat tgctggccca gcagaatatt
     1321 gaagtagttc gaagtggtcg tcgaacccgt cgaaggcagc gccgcttgtt gcaccagatc
     1381 gttctgtgtg tggttgcgat ccagcgatcc gcgcaagaac cattgcgagc gttccgattg
     1441 cgcccaatcc accctcccca tgaaaagtgt gtcccgacac ggcaccggca cattcgtggg
     1501 cacagcgatg gacggaacag cgggaatcag tccgtccgcc gcgagttgcc ctagcgcccg
     1561 aaactcagtt tgtgagttcg cgctgtaggc gacgctggca ttttcgtcca cgtattcgta
     1621 cgacgagaaa aaccacagct tgtcacgccg aagcggtcct ccgatcgtcg ctaccccgtt
     1681 ctgcctcgca aaaggctgtt tcggatccgg ttccggattg tccagtgggt tccgcgcgtt
     1741 caggctcttg ccgcgataat agtaggaagc gcttccgtgc cagtcgttcg aaccgcgccg
     1801 agtggacaaa ataatgcttc caccgttggt acgcgaagtg tctgctccga actgtgccga
     1861 ccgcactaca aattcctgca tcgcctcggg cgaatagttc tgcaaaaatc cgccgataaa
     1921 gtcgtcgttg ttgtcgccgc cgtccacgga caaatcaacg ttcagtcccg agcttccacc
     1981 gaacgataca gccgtgatcc gcgccttcgt tggatcggac ggctcgactg gcgcggtgaa
     2041 cggcgccatc agcgcaatgt tcgcaaaact ccgcgcggaa agcggtactt cgtcaatgtc
     2101 attcgccgtg attagggtct gcaccgtgct ggaagttgtt tccagcggct ggcttgctag
     2161 cgagggtccg cgatcgtgta cttccacaga atggcgcacc gattcaggac gcagggtcac
     2221 gcgcacggta ggccgcgaac cgatcgccac agtgatcttc tcggtttgcg cagcgaagcc
     2281 cacggccgtc acggtcaagg agtaaaccga gggtggaatc gcctgaaagc gcgcttcgcc
     2341 cagcgaattc gcagtaaccg actgggccag gcccgaaccc gcgtcagtca gctctgcctt
     2401 cgccgatgga atggccccgc cggatgcgtc tcgcacctcc accagcaacg agccgcgaaa
     2461 ctcctgggcg cgcgcgcaca gcgcgaacat cgcccacaaa cacaatggga cccggaaaag
     2521 tttagccatt aaaagagacc acttcacttt cgccaacaca cgattgtagg actagacgcg
     2581 agccgtcgaa aaaaggtttc tcgctatcgc gtttaatgct gcaaatgcgc gtcaaaccat
     2641 gccaccgtcc gccggatacg atcgcgcagg tgctcgggct ttacaaagtg atgcccttcg
     2701 tttttgtaga ccaccagttg cgtttccacg ccgagcgttt tcagcgcgtg ccagaattcg
     2761 tacgattgcg gcgttggaca ctcgccgtcg ctgtcgccca caatcaccag cgtcggcgtc
     2821 ttcacgttct tgatgaacgt aatcggcgaa ctcttggcgt aaaccgcggg atcgtcgtag
     2881 accggtgcgc caaaatacgg aatcatccac tggtcaatcg agttctgtcc a
//
LOCUS       DASTPM010000150         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_869969, whole genome shotgun sequence.
ACCESSION   DASTPM010000150 DASTPM010000000
VERSION     DASTPM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869969"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFR42_02655"
     CDS             <1..309
                     /locus_tag="VFR42_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368082.1"
                     /translation="IEKETGKLLGTAQLHFRQRCDHRTHCTPCLANLASSDGNAEQGH
                     TPSGEERSTKPAKRIGKWQELKAIKTNGLESSASTLAVQPSQPETLLSHAWGWIRRLV
                     "
     gene            complement(301..1707)
                     /locus_tag="VFR42_02660"
     CDS             complement(301..1707)
                     /locus_tag="VFR42_02660"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0368083.1"
                     /translation="MSDSLKVRLLVVDDEQSIRKLCMTIGGSLGYSCAEAESAEVALS
                     RIEQEAPDLILTDLKLPTQSGVELLRQAKSLFPRTEIAIMTGHGSIESAVDAMKLGAY
                     DYIEKPFRVEKMRLLLQRMAEKVRLVTENEFLRERVNTEDNLDGIVGASANIQDVLRM
                     ISRLKDTRTPVLVAGESGTGKELVARAIHFRGAMAQTPFVAVDCGSLVPTLMESELFG
                     YEKGAFTGAMKSKAGLFQAANGGTIFLDEIGELPLEMQAKLLRVLQEREVRPVGSNSA
                     INVDVRVIAATNRDLEAAYRAGTFRKDLYFRLNVVTVHLPSLRERRSDIPMLVHHFLN
                     RYAPGTNLQVTPAAMKSLLQYEWPGNVRELENCVARAITLGDRQLIDVADLPPAIRTE
                     SSDTTPRSSLDNNSLSTTALAEMERMTILKVFEQANGDKALAGRMLGISRATLYRKLK
                     RYNISLRPGPGEPVSTSN"
     gene            complement(1704..2087)
                     /locus_tag="VFR42_02665"
     CDS             complement(1704..2087)
                     /locus_tag="VFR42_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0368084.1"
                     /translation="MTPALNPILLIEDEPSVTAFVRAALERHGYTVVPAASGAEGLAR
                     LSENSFGGVISDIRMPGSVNGAEVHDWIRAHRPELQQRVILISGDTANSKTQALLAGS
                     GIPCIEKPFRVQQLISVVESTFGKP"
     gene            complement(2095..4401)
                     /locus_tag="VFR42_02670"
     CDS             complement(2095..4401)
                     /locus_tag="VFR42_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0368085.1"
                     /translation="MPETPRVRKASRRAADFIELATEAASFQQLPDFLDNFSRRVACT
                     LSAKWCGVVVFSGRSAKLYSAMPADAVGLDENALLDRANGAALHCNQKSAFQAFSFHI
                     SDGDSPELFVIPVRASNEEALGAICVYGASPAIFKRETKLLRALANHAALSLEASLRF
                     SQLERSKRQWVQDIDAISDYIVVHDRNWKIVRTNRSLADYLGRSPVELVGQPVSSLRR
                     LALGDAELPCPFCLNAGLNKEEHTVSSAEKIYLVSTSRASGATEEDARIIHVLKDITD
                     RREAERRYRELYDSIQEGLFFATPDGRFLDVNDAMVRMLGYSSREELLRDDVGSYLYP
                     EPGARSRLLSAMRKTGVLRNHEETLRRKDGRLLHTLQNITAVRDSHGKIKQFRGLMLD
                     VTEQKMFQSQLQRQRDFNQKILNATQSMIMVLDTAGLISYANRRCYEAGYKQDEIIGR
                     RLVDLVDSSQREEFDSSLETTAHGQQVENLELRAKRSDGTLGHFSISLSPMRDEQNAV
                     NSVVVVMTDITDAALMQAKLAHAERMATIGRLVSGVAHEVNNPLAAILGFTDLLLENP
                     QVPANAREDLQIILQETQRTKDIVQDLLSFARQRPVKRELAHINAVLRQTTKLRSYDF
                     ASHGVEVTEEYDENLPTVLGDAQQLQQVFLNLLNNAYDATEDAGRRGKIHLKTLHTSE
                     WVEVTIRDNGTGITDVERIFDPFYTTKQTGKGTGLGLSICYGIVRAHGGEIQCWNNTD
                     GEGSTFSIRVPVANQNANATASAKQAGR"
BASE COUNT          988 a         1258 c         1442 g         1032 t
ORIGIN      
        1 atcgagaaag aaacgggcaa gctgctcggt acagctcaac tccatttccg tcagcgttgt
       61 gaccatcgaa ctcactgcac cccctgtctt gctaacttgg ccagctcaga cggcaacgcc
      121 gagcaaggac acacaccgag cggagaagaa agaagcacga agccagccaa aagaattgga
      181 aagtggcagg agttgaaggc tatcaaaaca aatggcttag aaagttccgc cagtactttg
      241 gctgttcaac cgtctcagcc tgaaacgctt ttgtctcacg cttgggggtg gatacgacgg
      301 ctagtttgag gttgagacag gttcgcccgg gccaggacgc aatgaaatgt tgtagcgctt
      361 gagcttgcga tagagcgtgg cgcgactgat gccgagcatg cggccggcga gcgccttgtc
      421 tccattggcc tgttcgaaga ccttgaggat ggtcatgcgc tccatttcag ccagtgccgt
      481 agtggacagg gaattgttgt cgagcgaaga gcgtggcgtg gtatcgctgc tctcagtgcg
      541 aattgcggga ggcaagtcgg cgacgtcgat cagctggcga tcgccgaggg tgatggcgcg
      601 cgcaacgcag ttctccagtt cacgcacgtt gcccggccat tcgtattgca gcaggctctt
      661 catggcggcg ggagtgactt gcaggttggt gccgggagcg tagcgattga gaaagtggtg
      721 caccagcatg ggaatgtcgg agcgacgttc gcgcaaagaa ggcaggtgga cggtaacgac
      781 gttcaagcgg aaataaaggt ccttgcgaaa tgttccggct cgataggcgg cttcgagatc
      841 gcgattggtt gcggcgatga cgcggacgtc cacgttgatg gcagagttgc tgccgaccgg
      901 gcggacttcg cgctcttgca gcacgcgcag cagttttgcc tgcatttcca ggggaagttc
      961 gccgatttca tcgagaaaaa tagttccgcc attcgccgcc tgaaacaggc cggctttgga
     1021 cttcatggcg ccggtgaagg cgcccttttc gtagccgaaa agttcgcttt ccatcagcgt
     1081 gggaaccagg gagccgcagt ccactgcgac aaagggagtt tgcgccatgg cgccgcgaaa
     1141 atggattgcc cgtgctacca gttccttgcc cgtgccgctt tcgcccgcga ccagcaccgg
     1201 cgttcgcgtg tctttcaggc gagaaatcat ccgcaaaacg tcttgaatgt tggcggaggc
     1261 gccgacgatc ccgtccaggt tgtcttccgt gttgacgcgc tcacgcagga attcgttttc
     1321 ggtgaccagg cggacttttt ccgccatgcg ctgcaggaga aggcgcattt tctcaacgcg
     1381 gaaaggcttc tcaatatagt cgtaagcgcc aagtttcatg gcgtcgacgg cggattcgat
     1441 ggaaccgtgc ccggtcatga tggcaatttc agtgcgcgga aaaagcgact tggcttggcg
     1501 gagaagttcc acaccggatt gtgtcggtag cttcagatcg gtgaggatga ggtcgggagc
     1561 ttcctgttcg atgcgcgaca acgcaacttc cgcgctttcc gcttccgcac aggaatagcc
     1621 caaagacccg ccaatggtca tgcaaagttt gcgaatgctt tgctcatcat ccaccaccaa
     1681 caaccggact tttagcgagt cgctcatggt tttccgaagg tactctccac aacagagatc
     1741 aattgttgta cgcgaaaagg tttttcgatg caggggattc cgctgccggc gagtagcgcc
     1801 tgggtcttac tgttggcggt gtcgccgctg atcagaatca cgcgctgttg aagttcgggg
     1861 cgatgcgcgc gaatccaatc gtgaacttcc gcgccgttca ccgaaccagg catccgaata
     1921 tcagagataa cgccgccgaa actgttttcc gaaaggcgcg ccaggccttc ggcgccggaa
     1981 gcggccggta cgacggtgta gccgtggcgt tccagagcgg cacgcacaaa ggcagtgacc
     2041 gatggttcgt cttcaatcag cagaatgggg ttgagagcag gtgtcatcgc gaggtcacct
     2101 gccagcctgt ttggcggacg cggtggcatt ggcgttctgg tttgccacgg gaacgcgaat
     2161 ggagaacgtg ctgccttcac cgtccgtgtt gttccagcac tgaatttcgc caccgtgcgc
     2221 gcgaacgatg ccgtagcaga tgctcaggcc aagtccggta cctttgccgg tttgcttggt
     2281 tgtgtaaaag ggatcgaaga tgcgttcgac gtcagtgatg ccggttccgt tgtcgcgaat
     2341 ggtgacttcg acccactcgc tcgtgtggag ggttttcaag tggattttgc cgcgccggcc
     2401 cgcgtcttcg gtggcgtcat aagcgttatt caaaagattg agaaaaacct gctggagttg
     2461 ctgagcatcg cccaaaacgg ttggcagatt ttcgtcgtat tcttcggtca cctcgacgcc
     2521 gtggctggcg aaatcgtagc tgcgcagttt cgtagtttgt cgcagcacgg cgttgatgtg
     2581 cgccaattcg cgttttacag ggcgctggcg ggcaaagctg agcaagtcct gcacaatgtc
     2641 tttggtgcgc tgcgtttcct gcaaaatgat ctgcagatct tcgcgagcat tcgctggaac
     2701 ctgcggattt tcgagcaaca aatcggtgaa gcccaaaatc gcggccagtg gattgttgac
     2761 ttcgtgagcg acgccggaca ccaggcggcc aatagtagcc atgcgctcgg cgtgagccag
     2821 cttggcctgc attagcgctg cgtcggtgat gtcggtcatg actaccacga cgctgttgac
     2881 ggcgttttgt tcatcacgca tcggactgag actgatggaa aagtgtccga gcgtgccgtc
     2941 gctgcgtttg gcgcggagtt cgagattttc cacctgctgg ccatgggctg tggtttccag
     3001 cgaggagtca aattcctccc gctggctgga atccacaagg tcgaccaagc ggcgtccgat
     3061 aatctcgtcc tgtttatagc cggcttcata gcagcgacga ttggcataac tgatgagtcc
     3121 agcggtgtcc agaaccatga tcatgctctg cgtggcattc agaattttct ggttgaagtc
     3181 gcgctggcgc tgcagttggg actgaaacat cttttgctcg gtgacgtcga gcatcagccc
     3241 gcggaactgt ttgatcttgc catgtgaatc tcgcaccgcg gtaatgtttt gcaacgtgtg
     3301 caggagccga ccgtctttgc gacgaagagt ttcctcgtgg ttgcggagga cgccggtctt
     3361 gcgcatggca gagagcaggc gcgaccgggc tcccggctcg gggtacaaat aggagcccac
     3421 atcgtcgcgc agcagttctt cacgcgaaga atagccaagc atgcggacca tggcgtcgtt
     3481 tacgtcgaga aagcgcccat cgggtgtcgc gaagaaaagg ccttcctgga tgctgtcgta
     3541 gagttcgcga tagcgccgct ccgcttcacg gcggtcggta atgtccttta agacgtgaat
     3601 gatgcgcgca tcttcctcgg ttgcgccgct ggctcgagaa gttgaaacca aataaatttt
     3661 ttctgccgaa gaaacggtgt gctcttcctt attcaaccct gcgttcaggc agaaagggca
     3721 gggaagttcg gcatcaccga gagccagacg acgaaggctg cttacgggtt ggccgaccaa
     3781 ttcgacaggt gagcggccaa ggtagtcggc gagcgaacga ttggtgcgga cgatcttcca
     3841 attgcgatcg tgcaccacga tgtaatcgct gatggcgtcg atgtcctgaa cccactggcg
     3901 cttggagcgt tcgagttgcg aaaagcgcag cgatgcttct agagacagcg cggcatgatt
     3961 ggcgagagcg cgcaacaatt tcgtttcacg cttgaaaata gcaggggagg cgccgtaaac
     4021 gcagattgcg ccgagcgctt cctcgttgct ggctcgcaca gggattacga aaagctcagg
     4081 gctatcgcca tcggaaatgt ggaaactaaa agcctggaag gcggattttt ggttgcagtg
     4141 caacgccgcc ccgttggcgc ggtctaaaag cgcgttttcg tcgaggccca ccgcatccgc
     4201 tggcatcgcg ctgtacagtt tcgcagagcg gccgctaaag accaccactc cgcaccattt
     4261 ggcagacaag gtacaggcca cacgccgcga aaaattgtcc aaaaaatcgg gcagttgttg
     4321 gaagctggcc gcttcggtag ccagttcaat gaagtcagcc gcgcggcgcg aagctttgcg
     4381 cacacggggc gtttcaggca cgacgcgcgc taacctgcgg gccgacttgg gtttccgatt
     4441 tcgcaacgct tgagaatgtg gagatgccgt ctcaggatga acacatccac gcccttccac
     4501 gctaaacggc aatctcattc tgagtcaatt gcagaacgaa acggtgttgc tcatcacgct
     4561 acgctcccgg ccttccggcc aggggaggcg tctcagcctg agacctggcc gcgggacttc
     4621 cgagagtttg agacgaaaca cgtgcaccgg gcgtgctcag agcaagtaag cttgatcgca
     4681 taagtgatgt ttcctcagca agttccgggc aaattctcct
//
LOCUS       DASTPM010000151         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1129462, whole genome shotgun sequence.
ACCESSION   DASTPM010000151 DASTPM010000000
VERSION     DASTPM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129462"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFR42_02675"
     CDS             <1..389
                     /locus_tag="VFR42_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0368086.1"
                     /translation="AAISGAGEALNTSFYGHDEYQRDYETELGRPWENHALWDKLSPF
                     YKVANITTPTLFMGGDIDWNVPILGGEQMYQALKSLGRDTQLVVYPGEYHEFKAPTHL
                     KDRLERYLAWYAHYVKGDSAPATPKP"
     gene            563..1063
                     /locus_tag="VFR42_02680"
     CDS             563..1063
                     /locus_tag="VFR42_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368087.1"
                     /translation="MPDDLPMNPRDPASQPPATRRPNAPLWLGFLITMFAFLSYIPIF
                     AKYPITRDVPWVNYLIFALGLPLLFAGVLRAFRQPTIYRGKIAGPILATLGVLSLAFF
                     CLSIFSLGKHLPPSAQAPKVGQKAPDFALNDANGATVSLASLLATPGAAHPPKGVLVI
                     FYRGYW"
     gene            1076..1405
                     /locus_tag="VFR42_02685"
                     /pseudo
     CDS             1076..1405
                     /locus_tag="VFR42_02685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(1588..>2286)
                     /locus_tag="VFR42_02690"
     CDS             complement(1588..>2286)
                     /locus_tag="VFR42_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU0368088.1"
                     /translation="AVCKAEELWFHVDGAYGALSRLSEKLRGVVDGIELADSLAFDLH
                     QWMYLPFEVGCVLVRDADAHVRTFAAKQNYFGDGERGVIGGGLPFAERGIELTRSFRA
                     LKVWMSLKAHGVAAYARLIEQNVVQAAYLAELIGREDEMELLAPVTLNVVCFRARPRG
                     IAEAELNALNKEILLRVQESGAAVASSTVLDGKFAMRVAITNHRSRREDFGVLLEAVR
                     EMAGRVAAESVKRG"
BASE COUNT          458 a          826 c          528 g          474 t
ORIGIN      
        1 aagccgccat ctccggcgcc ggggaagccc tcaacaccag tttttacggc cacgatgaat
       61 accaacgcga ctacgaaacc gaactgggcc gcccgtggga aaaccacgcc ctatgggaca
      121 agctctctcc tttctacaag gtagccaaca tcaccacccc aactcttttc atgggcggcg
      181 atatcgattg gaatgtcccg atcctaggtg gcgagcaaat gtaccaggcc ctgaaaagtc
      241 tgggccgcga tacccagcta gtcgtctatc ccggcgaata ccacgaattt aaagcgccga
      301 cgcacctgaa agaccgtctc gagcgttatc tcgcctggta cgcccactac gtaaaaggcg
      361 actccgctcc ggccacgccg aagccttgac tttgtccagt cgttgtaggg gccgcgcttc
      421 agtttagccc gagcttcgag gggcgcgccc cagcgagcga agggaggcat ttattcttaa
      481 tccaaaccgc ggtccgaatt tgtgttagcc tttgccatcg cacacaagtt cggcatccgc
      541 aacatcctta aaggtctacg caatgcctga tgatttgccg atgaacccaa gagaccccgc
      601 ttcgcagcct ccggctacgc gccgccccaa cgcccctctc tggctgggct tcctcatcac
      661 catgttcgct ttcctcagct acatcccgat ttttgcgaaa tatcccatca cccgcgatgt
      721 gccttgggtc aactatctga tctttgcgct cggcttaccc ctgctcttcg ccggcgtcct
      781 ccgcgccttc cgccagccga ccatttatcg cgggaaaatc gccggaccca ttcttgccac
      841 cctcggcgtc ctatcgctcg cctttttctg tctatcgatt ttttctttgg gcaaacactt
      901 gccgccttcc gcgcaagctc ccaaagtcgg tcagaaagct cccgactttg cgcttaacga
      961 cgccaatggc gccacggttt cgctcgcatc gctgctcgcc acgcccggcg cggcgcaccc
     1021 gccaaagggt gttctcgtca ttttctatcg gggctattgg tgaccgttct gcaactccga
     1081 gttacggggt attcaggaaa gcctctctga tctggaaaag ctgggcgtaa cgccggtggc
     1141 catcagcgtc gattctcccg cggagtccaa ggatctctgc cgcaaagccg gttacaccta
     1201 cccgtttctt tctgacccca acgccgaagt cattcgccgc tacgacgtcc tgcacacagg
     1261 cgccggcatc aatcaccggg acattgcccg cccggccgaa ttcctgatcg attccaccgg
     1321 caccattcgc tggatcaatc tcaccggcga catccgcatc cgcgcccgcc ccgacgaaat
     1381 cctgcaagcc gccaagtctc tgtagccgtg cccagcgttg taggggtgcg cttcagcgca
     1441 acccagcgag cgaagcgagt cttttcgtgt tcctgtcatc tcgaccggag cgggccagct
     1501 ttttcttgcg ctcgggttat gagcgctggc ccgcgcagtg gagagatcac ggcaccactg
     1561 actgagtgcc ggaagaccgg tctaaattca acctcgcttc acactttccg ccgccactct
     1621 ccccgccatc tccctcaccg cctccaataa aaccccaaaa tcctcccgcc ggctccggtg
     1681 attggtaatc gccacgcgca tggcaaattt cccgtccagc actgtgctcg acgctaccgc
     1741 cgctcctgat tcctgcaccc gcagcaaaat ctccttgttc aacgcattca actctgcctc
     1801 tgcaatgccg cgcggccgcg cccgaaaaca aaccacattc agcgtcactg gcgccagcaa
     1861 ttccatttcg tcctcgcgcc caatcaactc cgccagatac gccgcctgca ccacgttctg
     1921 ctcaatcaac cgcgcgtacg cagcaactcc gtgcgctttc agcgacatcc acaccttcag
     1981 cgctcgaaaa ctccgtgtca gctcgatgcc acgctcggca aacggcaacc cgccgccaat
     2041 cactccgcgt tctccatcgc cgaaataatt ctgcttggct gcaaacgtcc gcacatgcgc
     2101 gtccgcgtcg cgcaccagca cgcatccgac ctcgaacggc aggtacatcc attggtgcag
     2161 atcaaacgcc agcgaatccg ccaattcgat tccgtccacc actccgcgca atttctcgga
     2221 caaccggctc aacgccccgt acgctccgtc cacgtgaaac cacaactcct ccgctttgca
     2281 aacagc
//
LOCUS       DASTPM010000152         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_725811, whole genome shotgun sequence.
ACCESSION   DASTPM010000152 DASTPM010000000
VERSION     DASTPM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725811"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1695
                     /locus_tag="VFR42_02695"
     CDS             280..1695
                     /locus_tag="VFR42_02695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0368089.1"
                     /translation="MHLLGLRRIAQATVVAGALAFGAGSLRAEPPATELQIPVEYYKL
                     PNGLRVVLSQDHTAPTVCVAVYYRIGFRVEPKDRTGFAHLFEHMMFQGSQNLGKMEFI
                     KLVQQNGGILNGSTRFDFTNYFEILPSNKLETALWAEADRMNGLAVTEDNLKNQQGVV
                     GNEVKVNVINRPYGGFPWLDMPQYANTNWYNAHNFYGDLKDIDAATLTDVQAFFKTYY
                     APNNAALAIVGDFDVVQARQWVEKYFGGIKASDLPAQPDLSEPKQTKEKNASRVDALA
                     KRPGIAIAYHMPERNTPEYYAMGLIDQILIEGDDSLAHQELVQKQGLTAQVQGGINYL
                     GNMFNYKGPMLFMADLRYDAATKPETIVKAWDTAAEPLRAKSVDKAILDRARVKLRSD
                     LYDNIGSVGGFGLADLLASFALFDDNPARINTLVSEFDKVTPELIQKTAQEYLKPENR
                     TVLVLEVKAEAAKPAEAPAKN"
     gene            1705..>2702
                     /locus_tag="VFR42_02700"
     CDS             1705..>2702
                     /locus_tag="VFR42_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0368090.1"
                     /translation="MKMNTKKIAFAVATIALCTLPLAAQEKQTPPAGGPPKPFNVPAH
                     ESYALPNGMKVTLVPYGNLPKVTLSVVVRSGNLNDPTDMPGLADIAGKLMKEGTASRS
                     SRQIAEEAAGMGGAVNITVGADESDATTDVLSEFGPKAAALLADVVEHPLFPDSELPR
                     LKNDAQRQLTISKSLPQSMALEKFRKILYGDHPYAAVFPTQESIEKTTVADIKKFYEG
                     NFGATRAHLYVAGRFDTAEMKKAIAASFGGWTKGPAAVTNVPKVKPQHVVDLTDRPGA
                     AQSTLLVGMPVPDATSPDAIPLGVTNALLGGSFGSRITSNIREQKGYTYSPSSQISR"
BASE COUNT          634 a          703 c          876 g          489 t
ORIGIN      
        1 tatgtggcgg ggcaactgct ggacgaagct aagccggagc cgcgcttttc cctggcgagc
       61 aaggagaccg tgcagaaacg ggcggtttac taggctcgcc agcgaaagct gggacgacct
      121 cggcatgcgc agatgaacag actgaagtct gtgctaccta aggcggggcc gggcgaaacc
      181 atttttcgcc cgatacgtct gtctagcatg aggcggagtg tggtcatttc ctttcgttcc
      241 tattggcagt aaattcaagc ggcagttttt ggaggcggta tgcacttgct aggtttgcgg
      301 aggattgcgc aggcgacggt ggttgcaggg gcgctggcgt ttggcgcggg cagcttgcgg
      361 gcagagccac cggcgacgga gctgcagatt ccggtggagt actacaaact gccgaacgga
      421 ttgcgggtgg tgctttcgca ggatcacacg gcgccgacgg tatgcgtcgc ggtgtattac
      481 cgcatcggat ttcgcgtaga gccgaaggac cggacgggct tcgcacattt gttcgaacac
      541 atgatgttcc agggctcgca gaacctgggc aagatggagt tcatcaagct ggtgcagcag
      601 aacggcggca tcctgaacgg atcgacgcgc tttgacttca cgaactactt tgagattttg
      661 ccttcgaaca aattggagac ggcattgtgg gccgaagccg accgcatgaa tggtttggcg
      721 gtcaccgaag acaatctgaa aaatcagcaa ggcgtggtcg gcaacgaagt gaaagtcaat
      781 gtgatcaacc ggccgtacgg gggattcccg tggctggaca tgccgcaata tgcgaacacc
      841 aattggtaca atgcgcacaa tttctacggc gatttgaagg acattgatgc ggcgacgctg
      901 accgacgtgc aggcattctt caagacgtat tatgcgccga acaacgcggc gctggcgatc
      961 gtaggcgact ttgacgtggt gcaagctcga cagtgggtgg aaaagtattt tggcggaatc
     1021 aaagcatcgg acctgccagc acagccagac ttgagcgagc cgaagcagac gaaagagaaa
     1081 aacgccagcc gcgtggatgc actggcgaag cggccgggca tagccattgc ataccacatg
     1141 ccggaacgca acaccccgga atactacgcg atgggtttga tcgatcagat cctgatcgag
     1201 ggtgacgaca gcctggcgca tcaagagctg gtgcagaagc agggattgac cgcgcaggtg
     1261 caaggcggaa tcaattacct gggcaacatg ttcaactaca aggggccgat gctgttcatg
     1321 gcggatctgc gctatgacgc ggcgaccaag ccggaaacga ttgtgaaggc gtgggatacc
     1381 gcagcggagc cgctgcgagc gaagtcagtg gacaaagcga ttctggatcg ggcccgcgtg
     1441 aagctgcgct ccgacctcta cgacaacatc gggtccgtgg gcggattcgg tttggccgac
     1501 ttgctggcca gcttcgcgct tttcgacgac aaccctgcgc gcatcaacac gctggtatct
     1561 gaattcgaca aagtgacgcc ggagttgatc caaaaaacgg cgcaggaata cttgaagccg
     1621 gaaaaccgga cggtgctggt gctggaagtg aaagcggaag cggcgaaacc ggccgaagcg
     1681 ccggcgaaga actaggagag cgaaatgaaa atgaatacga agaagattgc attcgctgtc
     1741 gcaacgatcg ctctttgcac tttgccactg gccgcgcagg aaaagcagac tcctccggcg
     1801 ggcggtccgc cgaaaccgtt caatgtgccg gcgcacgaaa gctatgccct gccaaacgga
     1861 atgaaggtga cgctggtgcc ttacgggaac ttgccgaaag tcacgctcag cgtggtggtg
     1921 cgcagcggca acctgaacga ccccaccgac atgccgggac tggccgacat cgccggaaaa
     1981 ctgatgaagg aaggaaccgc ttcgaggagc tcgaggcaga ttgccgaaga ggccgccggg
     2041 atgggcgggg cggtgaacat taccgtgggg gcggacgaga gcgatgcgac gactgacgtg
     2101 ttaagcgagt ttggtccaaa ggccgcggct ttgcttgcgg atgtcgttga gcacccgctc
     2161 ttcccggatt cggaactgcc gcggttgaag aatgatgcgc agcgacagct gaccatttcc
     2221 aaatcgctgc cgcaaagcat ggcgctggaa aagttccgca agattctcta cggcgatcat
     2281 ccatacgcag cggtgttccc aacgcaggaa agcattgaga aaaccaccgt ggcggacatc
     2341 aagaagttct acgaggggaa ttttggggcg actcgcgcgc acttgtacgt tgcgggaaga
     2401 ttcgacacgg cggaaatgaa gaaagcgatc gcggcaagtt ttggcgggtg gaccaaaggc
     2461 ccggcggcgg tgacgaacgt acccaaagtg aagccgcagc acgtggtaga tttaaccgac
     2521 cggcccggcg cggcgcagtc tacgctgctg gtgggcatgc cagttcccga tgcaaccagc
     2581 cccgacgcga ttcccctggg cgtgaccaat gccttgctgg gcggctcgtt tggatcgcgc
     2641 attacgtcca acattcgtga gcagaagggc tacacatact cgccgagcag ccagatttcg
     2701 cg
//
LOCUS       DASTPM010000153         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_783752, whole genome shotgun sequence.
ACCESSION   DASTPM010000153 DASTPM010000000
VERSION     DASTPM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783752"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFR42_02705"
     CDS             <1..462
                     /locus_tag="VFR42_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368091.1"
                     /translation="KANPVGYWLYKEKGYLQLSSSPEVLSLSDTGRTASKGWTLVALP
                     GKEVPGALERWEVPLAAKKFVAITNVLREQRMGVQVAQVTYSWVYSLTPLGTELFKSE
                     RIPSAGRGNESSAPADLTGIDLKKTYEGKAIFTLVRGSWHLEDDCRPLDVC"
     gene            complement(569..886)
                     /locus_tag="VFR42_02710"
     CDS             complement(569..886)
                     /locus_tag="VFR42_02710"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0368092.1"
                     /translation="MARVHSDHNAPVLPFRGLIIHQNEPARQRARELVNTPEFAKAQR
                     QRKKVEALFAELKNQIGLRRLRLRRLKFVREQFFLAAIAQNIKRLVRFLSQPTIPVLL
                     TTA"
     gene            complement(1045..1965)
                     /locus_tag="VFR42_02715"
     CDS             complement(1045..1965)
                     /locus_tag="VFR42_02715"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0368093.1"
                     /translation="MLRWISIFGGLLIMMGQHDRSEALFYYFRLEDQVPETHLLRLVE
                     KHISFAFVRERLKQSYSETGRPSIDPELLLRILLIGYLYGVTSERKLVEELRMHLAWR
                     WFTGLSFDQEIPHHSTFSKNRHGRFQQSKLFEELFEQIVRQCVGVGLVEGKHLSVDGS
                     FVEANASKESRIPREQLAEAAQVNRTVRQYVAELEEQNPLEEPVHQQDQVSTTDPDST
                     YATKGGTPARLGYLRQLSGGQSQLCDRGGASDGSAHEPGDGCGTRHAHPLRSMAGTRA
                     CHGRCRHDLRERRVSAMASRSEHHSLHADP"
     gene            complement(2297..3379)
                     /locus_tag="VFR42_02720"
     CDS             complement(2297..3379)
                     /locus_tag="VFR42_02720"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0368094.1"
                     /translation="MKIVGVDLHAKQQNIAMLDTETGELTEKLIRHEGNVVREFYVGL
                     EGPVVVGLEATGSMQWFLELLDELGIQYRVGHPAKIRAAEPRKQKHDRRDARLVLSLL
                     ERDHFPDIWMPSTEQRDLRTLLRDRHQWVKMRARLQHTLQSIALNHALRKGTSLWTAA
                     GQDSLQRLPLPPYTRQRRNQLLSLYAELQRRIQELDRQVDEQARQRPQARRLLTHPGV
                     GPVTALATEVFLGDPSRFTTAKQVASYIGMIPREYSSGQRQRLGNMTKQGNSLLRYLW
                     TEAAMHAVRNDPELKRFYRRKLIQKGMGKARIAAARKLGIRLWIMLRDQIEYEEFCRR
                     GKLRQNGEAHAGMPGFNSGPATTVTG"
     gene            complement(3610..>4039)
                     /locus_tag="VFR42_02725"
     CDS             complement(3610..>4039)
                     /locus_tag="VFR42_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEU0368095.1"
                     /translation="LMFVTADERDVIRLRTNYRKDEEVYMYRVRVQPEVARAMFLTYI
                     TYLNKLKNQPEWYNAVTRNCTTTLDKQLAADVNNPHPWNYQIILNGTLDRLLYDRGRL
                     VAGGLPFPELKQREHINAAGQAADHSPEFSALIRVGRIGF"
BASE COUNT          824 a         1161 c         1082 g          972 t
ORIGIN      
        1 aaagctaatc cagtcggata ttggctgtac aaagagaagg gctatttgca actctcatcc
       61 tcaccagaag tactcagcct ttcggatacg ggcagaaccg cgtcaaaggg ttggaccctc
      121 gtagccttgc caggcaagga ggtgcccggg gcactggaac gatgggaggt ccctctggca
      181 gcaaaaaaat tcgtggcaat cacaaacgtc ctgcgagagc agcgaatggg agttcaggtt
      241 gcgcaagtga cctatagttg ggtctattct cttacgccat taggtactga gctttttaaa
      301 agtgaacgca ttccgagcgc cggaagagga aatgagtcga gtgcaccagc agaccttaca
      361 ggtattgact tgaaaaagac ctacgaaggc aaggcaatct tcacccttgt ccgtggtagc
      421 tggcacttag aggatgactg taggccgctg gacgtctgct gaaccaaacg tcatttggtg
      481 aaaaatctcg tgggtgtcga aaaagtccct caacagaacg ccttctcatc tgcgagcatg
      541 cctcgccggt ttttttgtct ccgttcgact aagcggtggt cagcagaacc ggtatggtcg
      601 gttggctgag gaagcgcacg agccgcttga tgttttgagc tatcgctgcc aggaagaact
      661 gctcgcgcac gaacttgagt ctccgcagac gcaagcgccg caatccgatc tgattcttca
      721 gttccgcgaa caaagcttcc accttctttc tttgccgctg tgctttggcg aactctggcg
      781 tgttgaccaa ctcccgcgca cgttgtcggg ctggttcgtt ctggtggatg ataaggcccc
      841 ggaagggcag cactggtgca ttgtggtcgg agtgaacacg ggccacactt tttgcgtgtc
      901 ccgatgtagt tgtaagcgcg gttccgatga acccaaccgc cgtagttcag gggctgaccg
      961 gcggggcaga tatagcgatt gttttccggt tcgtacgtga aacgctcggg gccaaagaat
     1021 gggctcctct ttcggtggat gctgtcacgg gtccgcatgt agggagtgat gctccgatcg
     1081 actagccatt gcagaaactc gccgttcccg taggtcgtgt cggcagcgac cgtggcaggc
     1141 tctcgtccct gccattgagc gaagcgggtg agcatgtctt gtgccgcaac cgtctcctgg
     1201 ctcatgcgcg ctgccgtcgc ttgcaccccc acgatcacac aactgtgatt gtccaccaga
     1261 tagttgtcgt aaatacccca gccgggccgg cgtgccgccc ttggtggcat aggtggagtc
     1321 gggatccgtg gtcgacacct ggtcctgctg atgcaccggt tcctccagcg ggttctgctc
     1381 ttccagttcc gccacatact gccgcaccgt gcggttgacc tgtgctgctt ccgctagctg
     1441 ctcgcgcgga atgcggcttt ctttggaggc gttggcttcc acgaagctgc cgtccaccga
     1501 caaatgcttg ccctcgacca gtcccactcc cacgcactgc cgcacgatct gttcaaacaa
     1561 ctcctcgaat agcttcgact gctgaaagcg tccgtgacgg ttcttggaga acgttgagtg
     1621 atgcggaatc tcttggtcga agctcagacc ggtgaaccat cgccaggcca gatgcatgcg
     1681 caactcttcc acaagtttgc gttcactggt gacaccatac agatagccga tcagcagaat
     1741 gcgtagcagc aactcaggat caatcgaagg tcggccggtc tcgctgtaac tctgtttcaa
     1801 cctctcgcgc acgaaggcaa agctgatgtg cttttcgacc agccgcagca aatgagtttc
     1861 cggaacctga tcttccaacc ggaaataata gaacagtgct tcagaacggt cgtgttgccc
     1921 catcatgatc agcagtcctc cgaagataga aatccagcgc agcatgggaa gcaatggttg
     1981 cttctaacac gctttggcca aatctcagct gctgatttca gtgacttttt caacatctct
     2041 aagtacgccg gcactgtcaa gatttttgcg gttatgaatt tcataatttg gagcacacta
     2101 ttcacggcag cgcaggtgca ctgtgttctt cgacaagtaa ctggcttcgt tccgtgatct
     2161 gtatccaacc aatgggctcg gcctttcgct ttcatgttcc ggtggcgtag ccccaccaaa
     2221 catctctccg gtcacgttcg atcaggatga gttatgcgaa ctcccctccc ttcggggagg
     2281 cgggtagcct attcactcac ccggtcactg tagttgcagg accactgttg aaaccaggca
     2341 ttcccgcatg ggcttccccg ttctgccgca gctttccccg acggcagaat tcttcgtatt
     2401 caatctgatc gcgcaacata atccagagcc gaattccaag tttccgtgcg gccgcgatcc
     2461 tggccttgcc cattcctttc tggatgagct tgcgacggta gaatcgtttc agctccgggt
     2521 cgtttcgtac ggcatgcatc gccgcttcgg tccacagata gcgcagcagc gagttgcctt
     2581 gcttggtcat gttccccagc cgttgtcgct gcccgctgct gtactcacgg gggatcatcc
     2641 cgatatagct ggcaacctgc ttggcggtgg taaagcggct gggatctccc agaaacacct
     2701 cggtcgccaa cgccgtcacc ggccccacac ctggatgcgt cagcaatcta cgcgcttggg
     2761 gcctttgtcg cgcttgctcg tccacttgtc gatccagttc ttggatccgc ctttgcagtt
     2821 cagcgtacaa gcttaatagc tgattgcgtc gttggcgggt gtagggtggg agaggcaatc
     2881 gctgcagtga atcctgcccc gctgccgtcc acagggaagt gccttttcgc agggcatgat
     2941 tgagtgcgat cgactgtaac gtgtgttgca atcgcgctcg catcttcacc cactggtggc
     3001 gatcccgcag cagggttcgc aaatctcgct gttcggtgga tggcatccag atatccggaa
     3061 agtgatcgcg ctctagcagg gacaaaacta agcgtgcgtc ccggcgatca tgtttctgtt
     3121 tccggggctc ggccgcccgg atcttcgccg gatgaccgac ccgatactgg attcccaact
     3181 cgtccagcaa ctccaaaaac cactgcatcg acccggtggc ttccaagccc accaccaccg
     3241 gaccttctaa gcctacgtag aactctcgta ccacatttcc ttcgtggcga attagctttt
     3301 ccgtcaactc gccggtttcg gtgtccaaca tggcgatgtt ttgttgcttt gcgtgcagat
     3361 cgacgcctac aattttcatc tcggcctcct tcgcccgagc ctttttggtt ggatcccata
     3421 aaagcttact cgggtcgtcg cgaggccgac gtacttatgt catcacccac aggcgattac
     3481 accaactgcg accccgggga gcatccccag gggcagcttc ctgcggcgtt tgtgagtgta
     3541 atcgcgatgt aacgcccaag aatggcttcg cccatgaaca cgagcccaaa gccgccgatt
     3601 cgccattact cagaaaccga tccgccccac tctaatcagt gcagaaaact caggagagtg
     3661 gtctgctgct tggcctgcgg cgttgatatg ctcgcgttgc ttcagctctg ggaatggaag
     3721 cccaccagcg accaatcgtc cccgatcgta tagcaaccta tccaacgtgc cgttcagtat
     3781 gatttgatag ttccaagggt gcgggttgtt aacatcagca gcaagctgtt tgtcgagcgt
     3841 agtcgtgcaa tttcttgtca ctgcgttata ccactcgggc tgatttttca atttgttcag
     3901 gtaggtgata taggtgagga acatggcccg tgctacctcc ggctgtaccc ggacgcggta
     3961 catgtaaact tcctcatctt tccggtagtt ggttcgcaat cggatgacat cccgctcgtc
     4021 ggcagttaca aacatcagc
//
LOCUS       DASTPM010000154         2348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_754917, whole genome shotgun sequence.
ACCESSION   DASTPM010000154 DASTPM010000000
VERSION     DASTPM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754917"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1756)
                     /locus_tag="VFR42_02730"
     CDS             complement(329..1756)
                     /locus_tag="VFR42_02730"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU0368096.1"
                     /translation="MTQKLQVMKFGGTSVGDATCIRRSAQIVATAAKERPVAVVVSAM
                     SGVTNRLIEAANRAEGGDREAGKALCESLRKQHFEALAALVADEAGGATVQGKIEEIL
                     SEAARLYEGTALLRELTPRTLDAISSLGERLCAPIFAATLKGLGARGAAIEATELIMT
                     DSFHGGAEPQTAETRKKSQARIGPLLQEKVVPVVTGFIGATADGALTTLGRGGSDYSA
                     TILGAALDAEEIIIWTDVEGVLTADPRLVSDARTIPEISYREAAELAYFGAKVLHPKT
                     LRPVLPAGIPVWIRNSFAPDQPGTKITAKGRSIGGGVKALTAIRDVALISVGGPGIVG
                     VTDVVGRTFTTTAELRAEVLLISQSSSQNDICFIVAKAHAQATLEALRREFAQDLTHD
                     VVEHITMDPEIAIVAVVGENMRGTPGVAGRTFHCLGQEGVNIIAIAQGSSETNISFVV
                     SDKSVKQALLAAHREFGLANASSSR"
     gene            1999..>2348
                     /locus_tag="VFR42_02735"
     CDS             1999..>2348
                     /locus_tag="VFR42_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0368097.1"
                     /translation="MAKPVLLTVDDDPEVLRAVERDLRKRYADRYRILRADSGATALR
                     SLETLRRRNESVALFLADQRMPEMSGVEFISQAIHVYPEAKRVLLTAYADTDAAIQAI
                     NQARINHYLLKPWDP"
BASE COUNT          446 a          791 c          620 g          491 t
ORIGIN      
        1 cccaccccac ttcaagtcgc tctcttccgt ttgttccact tactcttcga ggccgtttcg
       61 ggggcttgcc ctcggaagtc gtccagcagc aactctggca gcgggatcat cgaaatcgtt
      121 tggagccaca aacttccccg cgagtctccc gcgtggccgt gcccctgcag cgttaccggt
      181 attaaacgcg ccacctcgcg accgcggatc gtgattccta tcttctcgcc cgccatcacc
      241 cgttccagca atttttaaaa ctgcaccttc gcctcagtca cactcgcggt tttcattttt
      301 ggaaagccta atcaatcgac ggactgaatc atctagaaga cgaagcattt gcgaggccaa
      361 actcgcggtg ggcggccagc agggcctgct tcaccgattt gtccgaaacc acgaaggaga
      421 tgttggtttc cgacgagcct tgtgcgattg caatgatgtt cacgccttcc tgacccagac
      481 aatgaaacgt ccggccagcg acgcccggcg tcccgcgcat gttttctccc accaccgcca
      541 cgatggcgat ttccgggtcc atggtgatgt gctccaccac gtcgtgcgta aggtcttggg
      601 caaattcgcg acgcagagct tcgagagttg cctgggcgtg cgccttggcc acgataaagc
      661 aaatatcgtt ctgcgaagac gactgcgaaa tcagcagcac ttccgcgcgc agctcggccg
      721 tcgtggtaaa ggttcgtccg acaacgtcgg taacgcccac aattcccggg ccgcctacgc
      781 tgatgagcgc gacgtcgcga atcgccgtca gtgctttcac tccgccgccg atgctgcgcc
      841 ctttcgcagt aatttttgtg ccgggctggt ccggcgcaaa gctgttgcga atccacaccg
      901 gaatccccgc cggtaagact ggccgcagcg tcttgggatg cagcactttc gcgccaaagt
      961 aagccagctc cgccgcctcg cgataggaaa tttcaggaat cgtccgcgcg tcgctcacca
     1021 accgcgggtc cgccgtgagc acgccctcca cgtcggtcca gatgatgatc tcttcggcgt
     1081 ccagcgccgc gcccagaatc gtggccgaat aatccgaacc gccgcggccc agggtggtca
     1141 acgcgccgtc ggccgttgcg ccgatgaacc cggtcaccac cggcacaact ttttcctgca
     1201 gcagcgggcc gatccgcgcc tgcgacttct tgcgcgtctc cgcggtctgc ggctccgcgc
     1261 cgccgtgaaa cgaatccgtc attatcagtt cggtcgcttc aatcgccgcg ccccgcgcgc
     1321 ccaacccttt cagcgtggcc gcaaaaatcg gcgcgcacaa ccgctcaccg aggctcgaaa
     1381 ttgcgtccag cgtccgtggc gtcagttcgc gcagcagcgc cgttccctca tacagccgcg
     1441 ccgcctcgct tagaatttct tcgatttttc cctgcacggt tgcgccgccg gcttcgtccg
     1501 ccaccaaagc cgccagcgcc tcaaagtgct gtttccgcag cgattcacat aacgctttgc
     1561 ccgcctcgcg gtcgccgccc tcggcgcgat tcgctgcttc gatcagccgg ttggtcacgc
     1621 cgctcatcgc cgaaaccacc accgccaccg gacgttcctt cgccgctgta gccacgattt
     1681 gcgcggagcg ccggatacac gtcgcgtctc ccaccgacgt gccgccaaac ttcatcactt
     1741 gaagtttttg tgtcatgagt tcttttcggc tgattaaaaa tcataaccgc agccaccggc
     1801 agtgacttgc actttcttgc cggatgtttc aatttttgca gcatcgcaag cgcccgtggc
     1861 gcaggcgcgc accggccgat ttgtccattt cccgcgctgg gagtataaaa aagaggcgcg
     1921 ccaaattcct tgcgcatgtg tccgctgggc ctgttctggc atccaactgg aacacctgcg
     1981 taaaagagaa agaatgaaat ggccaaacct gtactgttga ccgtcgatga cgaccccgag
     2041 gttttgcgcg cggtggagcg cgatctgcgc aaacgctatg ccgaccgcta ccgcattctg
     2101 cgcgcagact cgggcgcaac ggcgcttcgc tcgctggaaa ccttaagaag gcgcaacgaa
     2161 tcggtggctc tttttctagc cgaccagcgc atgccggaaa tgagcggcgt cgaattcatc
     2221 agccaggcca ttcatgttta tccagaagcc aaacgagttc ttctgacggc atatgccgac
     2281 accgatgcag ccattcaggc catcaatcag gcgcgcatca atcattatct gctcaagccc
     2341 tgggaccc
//
LOCUS       DASTPM010000155         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_351492, whole genome shotgun sequence.
ACCESSION   DASTPM010000155 DASTPM010000000
VERSION     DASTPM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351492"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFR42_02740"
     CDS             complement(<1..637)
                     /locus_tag="VFR42_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0368098.1"
                     /translation="MSELAIRAEGLGKLYELGELERYSALRDSLARAVTKPWTFFRRR
                     KKETFWALQDVSLEVRHGEVLGLIGRNGAGKTTLLKILSRITKPTRGWAEIYGRVGSL
                     LEIGTGFHPELSGRDNIYLSGAILGMSKREIARKFDEIVAFAEIEKFLDMQVKHYSSG
                     MYVRLAFAVAAHLEPEILLVDEVLAVGDIKFQKKCLGKMGDVAKAGRTVVLV"
     gene            complement(1179..2069)
                     /locus_tag="VFR42_02745"
     CDS             complement(1179..2069)
                     /locus_tag="VFR42_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368099.1"
                     /translation="MIASMAYVTRDQPLTAQATQPVTTAATVRIEPPGRFLAFDLRET
                     WNYRELLYFFVWRDVKVRYKQTVIGVAWVVLQPLLSMGVFTMFFGRLAKLPSDGLPYP
                     LFYFAALVPWSYFATALSSATNVVVENQRVITRVYFPRLILPLASVFSGLLDFAIAFL
                     VLVVMVVGYGLRPGAQVLWLPFLLLLAVGTALAIGLWLSALNALYRDVRYVIPFVMQF
                     WLLASPVAYPSSLVPEKWRWLYGMNPMAGVIEGFRWALTGHGQPPGVLLLASASAVAV
                     LLVSGTMFFQRMEGEVADRV"
     gene            complement(2265..3344)
                     /locus_tag="VFR42_02750"
     CDS             complement(2265..3344)
                     /locus_tag="VFR42_02750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0368100.1"
                     /translation="MFLSVLVDTYNHQGLIEKALRSVLDQDYPPDGFEIIVVDDGSTD
                     STPEIVRQFEPRVRLIRKTNGGQASAFNTGIPQCRGDVIVFLDGDDWWAQGKLQRVAE
                     IFSTDRELGMLGHAFSESFDDGTERVIQPDQPANFRLDSASNANYFRLSRCYFGTSRL
                     ALRAELARKILPVPQSLVFEADEYLFTMASALAPGLVLAEPLTHYRVHGGNLFLAAGK
                     STEGERRKASVLATLAAALSKDLRATAASPEAIAMIVELVQAEADQLRLKLDGGWPWE
                     TFRTERTIYRIQHGDASWKSSAFRTLSMAPAMLLPPKWFYAGRAWLAAQSWYKRARAR
                     ALPVPGFTKLDAPAGSTSPNRKAPS"
BASE COUNT          804 a         1188 c         1032 g          713 t
ORIGIN      
        1 tcaccagcac caccgtccgt ccggccttcg ctacgtctcc catttttccc aaacattttt
       61 tctgaaactt gatgtctccc accgccaaca cctcatccac cagaagaatt tccggctcca
      121 gatgtgctgc caccgcaaag gccagccgca cgtacatgcc gctcgaataa tgctttacct
      181 gcatgtccag aaatttctca atttccgcga acgccacgat ttcgtcgaac ttccgcgcga
      241 tctcacgttt ggacatgccc aggatcgcgc cgctcagata gatattgtcc cgccccgata
      301 gctccggatg gaatcccgtc ccgatttcca gcaagctgcc cacgcggccg taaatttccg
      361 cccatccacg cgtcggcttg gtgatccggc ttagaatctt cagcagtgtg gtttttccgg
      421 cgccgtttcg accgatcagc cccagcactt ccccgtgccg cacttccagg ctcacatcct
      481 gcaatgccca gaacgtctct ttcttccgtc gccggaaaaa tgtccacggc ttcgtcactg
      541 cccgtgccag cgaatcccgc agagcgctgt accgttccaa ctcgcccaac tcgtacagct
      601 tgcccagtcc ctccgcgcgg atcgccagtt cgctcatttc ccgccagccg gcccgttcga
      661 aggcacttcg gaacgcaccg gcttttctgc gccgccgaat cgcaaaaacc gttcgtcgca
      721 gccagcgcac cggtatgctc gtatacccac cgggcgcaag atacttcgct caatcatgcc
      781 gtgcatctgc gatcgccgca gttctgctgt tcgacaccgg ggacacattc gcttaccgac
      841 cgacgtcttg ctttccatga cccacctcac ttacttcgcc ctgcctattt cgctgacttc
      901 gctaacctca tgcgcggtcc gctgttcccg cagccgcgac cgcgtcaccg aaacctcgaa
      961 atcccttgtt gtaaagcctt tagagtaact agttacaaag agcctgagct ctaagtcctt
     1021 tagaaccaat actgggaaaa acaggggatt aaggctaccg gcctcgcttc cagagccctg
     1081 ctgcagaaac ttgtcctgtt gccgcaacag ctttatttcc atttacttgc tgcgacagcc
     1141 gaggtggagg gtcaacccgc caacctcaga ttcgaccttc acacccggtc cgccacttct
     1201 ccttccatcc tctggaagaa catcgtcccg ctcactagca gcaccgctac ggcgctcgcc
     1261 gacgccagca gcagcactcc gggcggctgg ccgtgtcccg tcagtgccca ccggaaccct
     1321 tcgatcaccc cagccatcgg attcatgccg tacagccatc gccacttctc cggaaccagg
     1381 ctgctcgggt acgccaccgg cgaagccagc agccaaaact gcatcacgaa tggaatcaca
     1441 tatcttacgt cgcggtatag cgcgttgagc gcggaaagcc acagacctat cgccagcgcc
     1501 gtgccgaccg ccagcagcaa caaaaatggc agccacaata cctgcgcgcc cgggcgcaat
     1561 ccatacccga caaccattac aaccagcacc agaaaagcaa tcgcaaaatc cagcaggccg
     1621 gagaacacgc tcgcaagcgg caaaatcagc cgcggaaagt acactcgcgt gatgacacgc
     1681 tggttctcca ccaccacatt cgttgctgaa gaaagcgccg tcgcgaaata actccacggc
     1741 accagcgccg caaaataaaa caacggatac ggcaatccgt ccgacggcag cttcgccaaa
     1801 cgcccaaaaa acatggtaaa cacgcccatg ctcagcagcg gctggagcac cacccacgcc
     1861 accccgatca ccgtctgctt gtagcgcacc ttgacgtcgc gccacacaaa gaagtacagg
     1921 agttcgcggt agttccaggt ttcgcggaga tcgaacgcca ggaagcggcc cggcggttca
     1981 atgcgcacgg tcgctgcggt cgtcaccggc tgggtagcct gtgccgtcag gggctggtct
     2041 cgagtcacat acgccatgga agcgatcaat ccttcgcggg cgcggctgga cgtacccgca
     2101 tcttagggta gcaaaacagt ccgcggattt cgcgtgaatg gcgccgctgc gctcgccaca
     2161 tagtacttta tatattatat ttatatatat acaacagagc acacaactcc gcggtaggat
     2221 cgctagcgat ggcgcaccgc ggtgtcgagc gtcgcgcagg actttcagct aggcgccttc
     2281 cgatttggtg aagtgctgcc agccggggcg tccagtttgg tgaagccggg gacaggcagc
     2341 gctcgcgcgc gggcacgctt gtaccaggat tgcgcggcga gccaggcgcg gccggcataa
     2401 aaccatttgg gcggaagcag catagcggga gccatgctga gcgttcgaaa ggcactgctt
     2461 ttccaactgg catcaccatg ctgaatgcgg taaatggtgc gttcggttcg aaaggtttcc
     2521 cagggccagc cgccgtcgag ttttaggcgc aactgatcgg cttcggcttg caccaattcg
     2581 acgatcatgg cgatcgcttc gggcgaggcg gcggtggccc gcaaatcctt gcttaaggcg
     2641 gcggccagag tagcgagcac gctggccttg cggcgctcgc cttctgtgct cttgccggcg
     2701 gcaagaaaca gattgccgcc gtgcacacga tagtgggtga gaggctctgc caaaaccaga
     2761 cccggcgcga gggcggacgc catggtaaac agatactcgt cggcttcgaa gaccagtgat
     2821 tgcgggaccg gaaggatttt gcgcgcgagt tctgcgcgca gggcgagacg gctggtgcca
     2881 aagtagcagc ggctgaggcg aaagtagttg gcattcgaag cgctatcgag ccggaagtta
     2941 gcgggctgat cgggctgaat cacgcgttcg gtaccgtcat cgaacgattc gctgaaggcg
     3001 tggccgagca tgccgagctc gcggtcggtg gaaaaaattt cagcgactcg ctggagtttc
     3061 ccctgcgccc accaatcgtc gccgtcgaga aagacaatga catcaccgcg gcattgggga
     3121 atgccagtat tgaaagcgga ggcttggccg ccgttcgttt tgcggatcag acggacgcgc
     3181 ggttcgaatt gacgaacgat ttccggagtg ctgtccgtgg aaccatcgtc caccacgatg
     3241 atctcgaaac catccggtgg atagtcctgg tcgagcacgc tgcggagggc cttttcgatg
     3301 aggccctggt gattgtaggt atcgacaagg acgctgagaa acacgattga gagaagttcc
     3361 gatccgagtc cgatcccggc ctagggctgc ccggggttag gccggcgcgc caggcaatac
     3421 aggtacgagc caaactatcc ctccgccatc ttacataggg aattgcggca gcggccagcg
     3481 gaaaacaagc ggcgtgttgg acgcggccgg ggatctgcac cacgcaggta cggaaggaga
     3541 gaccgacggc attgtgcagt tcctccgggc tctcctgaat ttcgtcgcag aaccagccgg
     3601 ggagttccgg cgcgagaaac gccagagcgc agactgccgt tgatttgctg ggcgatacgc
     3661 gacggagctt tttcctgctg catggacagg aacggagaaa ccgtgcgcgc gcaacgccgt
     3721 gctgagcgaa tcaggat
//
LOCUS       DASTPM010000156         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1158677, whole genome shotgun sequence.
ACCESSION   DASTPM010000156 DASTPM010000000
VERSION     DASTPM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158677"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFR42_02755"
     CDS             complement(<1..341)
                     /locus_tag="VFR42_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368101.1"
                     /translation="MFTEIFKQAWSALGRNPVRSFLTMSGIAWGIVAVTLLLSYGSGF
                     RSVLMYTFEVFGKGAVVAWPGTTSEQAGGERAGKPVRFEAEDAEWVKAQSPLIRRVTR
                     ETVRFQSVSHN"
     gene            complement(345..1601)
                     /locus_tag="VFR42_02760"
     CDS             complement(345..1601)
                     /locus_tag="VFR42_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368102.1"
                     /translation="MSFRDLLRDTLVTLWAQKRRTLLTMFGIAWGIISITVMVAAGEG
                     LGDGIQKNQQTFGKDVMIVFSGRTSMQAGGSRAGRMVRWMEDDYIPVAAESPACKYVM
                     PELGNDVQARSLFNSGTIPVVGALPPFSEIRSVQPAQGRFYNNEDNENARNVAFLGSD
                     VKKQLFADREAMGQTVWMNGIPYTVIGVMKAKDQNSSYDGFDTRKIFVPFNSMKRDFP
                     NKPPAVEHTVDRLLIAPWSVETHKDCVRQLRRTLARLHNFDPRDEEAASIWDTVKNAQ
                     ANRMIIVGMEIFMGAVGIATLFLGGLSVMNVMLVSVRERTREIGVRMALGATRKSILR
                     QFFVETIFVVAISGGAGLIISYGFCALVNLAPMPPFFSGMLTSWKIAAMSVLLLGLIS
                     ILSALYPANRAASVDPIEALRFEAGG"
     gene            complement(1618..>2290)
                     /locus_tag="VFR42_02765"
     CDS             complement(1618..>2290)
                     /locus_tag="VFR42_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0368103.1"
                     /translation="MSAQRNLAVSKAEISKAQAQAAQAKAALDNADEDLRNSTIISPI
                     DGLVLSRDVNVGDAVSSILILGSQATPIMTLGDVSEVYVQGKVDEADIGKVYLNQPAR
                     IVVESFKDKKFEGKVTKISPLGKEKDNVTTFEVRVSISNFSGELKANMSANAEILLEE
                     KKSVLMAPEAAMIYDKDRNAQVEIPDAKGPNGKKKVSVKLGISNGVKTEILAGLNEKQ
                     QVVLQ"
BASE COUNT          471 a          789 c          514 g          516 t
ORIGIN      
        1 tcgttgtgcg aaacactctg gaaccgtacg gtttctcgcg tcacccgccg aatcaaggga
       61 gattgcgcct tcacccactc cgcgtcttcc gcctcaaatc gcaccggctt cccggcccgc
      121 tctccgccgg cctgctcgct ggtcgttccc ggccatgcca ccactgcccc tttgccaaac
      181 acctcaaacg tgtacatcag cacgctgcga aatcccgatc cataactcaa cagcagcgtc
      241 accgccacaa ttccccatgc aatccccgac atggtgagaa aactcctcac cggattccgc
      301 cccagcgccg accaagcctg cttgaatatc tccgtgaaca tggcttaccc gccggcctca
      361 aagcgcagcg cctcgatcgg atccaccgac gcagcccgat tcgcgggata aagcgcggat
      421 agaatcgaaa tcagccccaa cagcaagacg gacattgccg caatcttcca actcgtcagc
      481 atcccgctga agaatggcgg cattggcgcc agattcacaa gcgcgcagaa tccgtaagaa
      541 atgatcaagc cggcgccgcc actgatcgcc accacaaaaa tcgtttccac aaaaaactgc
      601 cgcaagatgg acttgcgcgt tgcccccagg gccatgcgca ctccgatttc acgcgtccgt
      661 tcccgaacac tcaccagcat cacattcatc acgcttagcc cgccgagaaa cagtgtcgca
      721 attcccactg cgcccatgaa aatttccatt cccacgataa tcatccggtt ggcttgggcg
      781 ttctttactg tgtcccagat gctcgctgct tcttcatcgc gcggatcaaa gttgtgcagc
      841 cgcgccagcg tccgccgcag ttgccgcaca cagtccttat gcgtctccac cgaccagggt
      901 gcaatcagca accgatccac cgtgtgttcc acagcgggag gcttgttcgg aaagtcgcgc
      961 ttcatcgaat tgaacggaac aaaaatcttg cgggtatcaa atccgtcgta actggagttc
     1021 tggtctttcg ccttcatcac gccaatcacc gtgtagggaa tcccattcat ccatactgtc
     1081 tggcccatgg cctcgcggtc cgcaaacaac tgcttcttca cgtcgctacc cagaaacgcg
     1141 acgttccgtg cattctcatt gtcttcgttg ttgtaaaaac gcccttgcgc cggctgtacg
     1201 ctgcggatct cgctgaacgg cggcaacgcg cctaccaccg gaatcgtccc gctgttgaac
     1261 aggctcctcg cctgcacgtc gttgccaagc tccggcatca cgtatttgca cgcgggtgac
     1321 tcggccgcca ccgggatgta gtcatcctcc atccaacgca ccatgcgtcc cgcgcgcgat
     1381 ccgcccgcct gcatgctggt ccgtccggaa aacacgatca tcacgtcttt gccgaacgtc
     1441 tgctgattct tctggattcc atcgcccagg ccttcgcccg ccgccaccat taccgtgatc
     1501 gagatgattc cccacgcgat cccgaacatg gtcagcagcg tccggcgttt ttgcgcccac
     1561 agtgtcacta gcgtatcgcg cagcaaatct cgaaaggaca cgattcgccc ccgttgccta
     1621 ttgcaaaacc acctgctgct tctcgttcaa cccggccaaa atttccgtct tcacgccgtt
     1681 ggagatcccc agcttcacgc tgaccttttt tttcccgttg ggccctttag cgtcgggaat
     1741 ttccacctgc gcgttgcgat ccttgtcgta aatcatggcc gcttccggcg ccatcaacac
     1801 gctcttcttc tcttccagca gaatttccgc gttggcactc atgttcgcct tcaattcgcc
     1861 gctgaaattc gaaatcgaaa cacgcacttc aaacgttgtg acgttgtcct tctccttacc
     1921 caacggagaa atcttcgtca ccttaccctc aaactttttg tccttgaagg attccaccac
     1981 gatgcgtgcc ggctggttca aatagacctt gccgatatcc gcctcgtcca cttttccttg
     2041 cacgtatact tcgctcacgt cgcccagcgt cataatcggc gtcgcctggg aacccagaat
     2101 caaaatcgaa ctcacggcgt cgccgacgtt cacatctcgc gaaagcacta gtccgtcaat
     2161 cgggctgata atcgtggaat tccgcaaatc ttcgtcagca ttgtccagtg cggctttggc
     2221 ttgagcggct tgcgcttgcg ctttggaaat ttccgccttc gataccgcca gattccgctg
     2281 cgcgctcatc
//
LOCUS       DASTPM010000157         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_265076, whole genome shotgun sequence.
ACCESSION   DASTPM010000157 DASTPM010000000
VERSION     DASTPM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265076"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFR42_02770"
     CDS             complement(<1..861)
                     /locus_tag="VFR42_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368104.1"
                     /translation="MKPRLLVVPHIYADNISVREIELARRFVPHFDVFLLKWPDALHV
                     DAKSVIGRRVRQASVALHAAWRRRMQSTDRDGLCLVEVPVWQPLLFHKLIGERAALKM
                     CQLRNRATLRGIVAELGISHILLANALFGMERIQGARTFYDVVDWFPEDASSIGKLAQ
                     IRGDLVAASRQMDGIFAVSQPLCEKLARDCDVEAVPLPNGADIAGLRGVAPARASGVR
                     RELGVAEDFVIGYIGNHGSYTGVDLVVDAFLAVRARIPNAKLLIVGPADYWHQLLQAN
                     RGAGVIATGAV"
     gene            complement(893..>2414)
                     /locus_tag="VFR42_02775"
     CDS             complement(893..>2414)
                     /locus_tag="VFR42_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0368105.1"
                     /translation="FSGVRADQLDEVCFGWSTAGRGYRHDLKCLATGGLPLTYLNVLT
                     STLYFLSMWHQEGGAKRFAQHFGSTKARMRFVDHHLAHAISAYAFSGFDDSAVVVMDG
                     RGAWEATSIWHGSKGKLRHVATIPFPNSLGWFYSEFTSYLGFQRNSDEWKVMGLAPYG
                     APGVRLDAFIEPEASPYRVNFKSLFGDGNGSFPGIIPILGPARLPESEIEDRHKNVAY
                     AVQDACELAMISVVRDAVERTRSRNLCLAGGVALNSKANGKIAASGLVDNLFVQPASS
                     DDGVALGAALAPYMDNGGALPNHAMRHAYLGPAFDDPAIETALRTYKLSYKRLDDPAA
                     TAAQFLSQSKILGWFQGRMEFGPRALGSRSILADPREPDMTAKVNNAVKFREWWRPFA
                     PSLKKESAGEFLESATDSPFMILTAQVRPEKRAVIPAVTHVDGSARPQTVEKETNPLY
                     WRLLDQFEKRTGVPVIMNTSFNLRGEAIVHTPTDAVRTFFSSGMDALVIGSFLVEK"
BASE COUNT          550 a          735 c          655 g          474 t
ORIGIN      
        1 gacggcaccc gtggctataa cgcccgcgcc ccgatttgcc tgcaacagtt ggtgccaata
       61 atcagcgggt ccgacgatga gcagtttggc attcggtatg cgtgcacgca cagcaagaaa
      121 ggcgtcgacc accaaatcga cgcctgtgta ggatccgtgg tttccgatgt aaccaattac
      181 aaaatcttcg gccacgccca gctcacgtcg cacgccggac gctcttgccg gtgcgacacc
      241 gcgcaggccc gctatgtcgg cgccattggg caagggtacc gcctccacgt cacaatcgcg
      301 cgcgagcttt tcgcaaagcg gctgggaaac cgcgaatatg ccatccatct gtctggaagc
      361 cgccaccaga tcgccgcgaa tttgagccag ttttccgatg gaactagcgt cctccggaaa
      421 ccaatcgacc acatcgtaaa aagtccgcgc tccctggatt cgttccattc cgaacaatgc
      481 gttggccaac aaaatgtgag aaattcccag ttcggcgaca atcccgcgca gcgtggcgcg
      541 attacgtagc tggcacattt tgagagcagc cctttcgccg atcagtttgt gaaacaagag
      601 cggctgccat accggaactt caacgaggca aagcccatcg cggtccgtcg attgcatcct
      661 tcggcgccaa gcggcatgca gagcgacact cgcttgccgc acgcgcctac caatcaccga
      721 cttggcgtcc acgtgcagcg cgtccggcca cttcaaaagg aatacgtcga aatgagggac
      781 aaaacggcgc gccagttcga tttcgcggac cgaaatgttg tcggcgtaaa tgtggggaac
      841 gaccaaaagt cgcggcttca cgggcgcttc gtccagtcca atcgcccttc actcatttct
      901 ccaccaaaaa gctcccaatt accagcgcgt ccattccgga actgaaaaaa gtgcgcactg
      961 catcagtcgg cgtgtgcacg atcgcttcgc cgcgcagatt gaaagaggtg ttcataatta
     1021 ccggcactcc ggtccgtttt tcgaattggt cgaggaggcg ccaatacagc ggattggttt
     1081 ctttttcgac ggtttgcggc cgggcgctgc cgtcgacatg ggttaccgca ggaatcacag
     1141 cacgtttttc cggcctgact tgcgcggtca gaatcatgaa tggtgaatcg gtggcgctct
     1201 ccaggaattc gccggctgat tcctttttta gcgaaggcgc gaaaggccgc caccactcgc
     1261 gaaacttgac ggcattgttc accttcgcgg tcatatccgg ctcgcgcgga tcggcgagaa
     1321 tcgagcggct ccccagagct cgcggaccaa attccatgcg gccctgaaac cagccaagaa
     1381 ttttactttg cgagagaaat tgcgcagcgg ttgccgcggg gtcgtccagg cgtttgtagg
     1441 atagcttata ggtacgcagg gccgtctcga tcgctggatc gtcaaacgcg ggaccgaggt
     1501 aagcatggcg catcgcgtgg ttcggcagcg cgccgccgtt atccatataa ggtgcaagcg
     1561 cggcgcccag agccacaccg tcgtccgagg aggccggctg cacaaaaagg ttgtccacca
     1621 aaccggaggc ggcgatcttg ccgttagctt tcgaattcag cgccacaccg ccggccaggc
     1681 aaagatttcg cgagcgcgtt ctctccaccg cgtcacgcac aacgctgatc atggccagct
     1741 cgcacgcatc ctgtacggca taagccacat tcttgtggcg gtcctcaatc tcgctttccg
     1801 gcaagcgtgc cggccccaaa attgggataa ttcccggaaa tgagccattg ccatcgccaa
     1861 acaggctctt gaaatttacg cgatacgggg atgcctcagg ttcgatgaaa gcatccaagc
     1921 gaaccccggg agcaccgtag ggcgccagtc ccatcacctt ccattcatcg ctattgcgtt
     1981 gaaatcccaa atagctggta aattcgctgt aaaaccagcc aagcgaattg ggaaaaggta
     2041 ttgtcgccac gtggcgcaac ttgcctttgc tgccgtgcca aatcgaagtc gcttcccacg
     2101 cgccacgtcc atccatcaca acgacagcgg aatcatcgaa gcctgaaaac gcataggcgc
     2161 taatcgcgtg ggccaaatgg tgatcgacaa aacgcatccg ggccttcgtg gaaccgaaat
     2221 gttgtgcaaa ccgcttcgcg ccaccctcct ggtgccacat gctgagaaaa tacagcgtgg
     2281 aagtcagcac attcaggtac gtcaggggca ggcctccggt ggcaaggcat ttcagatcat
     2341 gccgataccc gcggcccgcg gtgctccagc caaaacagac ttcgtcaagt tgatcagcgc
     2401 gcactcctga aaac
//
LOCUS       DASTPM010000158         5903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_466883, whole genome shotgun sequence.
ACCESSION   DASTPM010000158 DASTPM010000000
VERSION     DASTPM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466883"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..3360
                     /gene="carB"
                     /locus_tag="VFR42_02780"
     CDS             100..3360
                     /gene="carB"
                     /locus_tag="VFR42_02780"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU0368106.1"
                     /translation="MPKRKDIKKILIIGSGPIVIGQACEFDYSGVQACKSLRDEGYQT
                     VLVNSNPATIMTDPGMANRTYIEPLAKEYLEAIIAQERPDALLPTVGGQTALNLAVEL
                     AEGGILEKYKVEMIGASLRAIKVAEDRLWFKDACRKIGLEVPASALINNAQDAMRLCE
                     QLGFPLVIRPSFTLGGTGGAIAYNREEFGEAIQHALDMSPVHEALVEESVLGWKEYEL
                     EVMRDYRDNFVVICTIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAASAILREVG
                     VETGGSNVQFAVNPENGRMVVIEMNPRVSRSSALASKATGFPIAKIAARLAVGMSLDE
                     IPNDITKKTPASFEPTIDYVVTKIPKWQFEKFPGSDSTLGTQMKSVGEVMAIGRTFKE
                     SLQKAIRSLEPHTPWRAPADTPDSLLREKLATPRPDRIHWLLTAVERGLHAEEIGELT
                     KIDPWFLKQVEDLVAMNKRAAGVTVETASKELLREVKRHGTSDEQLAQMWKTTPAAVR
                     LQRARYGVAPVFKRVDTCAAEFESYTPYMYSTYEDEDESDATSKPKIMILGSGPNRIG
                     QGIEFDYCCCHASFALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLAIVE
                     REKPQGVIVQFGGQTPLNLALELKRNGVPIIGTAPESIDLAEDRRRFGRLLEELKIPQ
                     PRNATALVPEEAARLAGEIGLPVLVRPSYVLGGRAMVIAYDANTVQDYVAQAALMGPA
                     RPVLIDQFLEEATEVDVDALADGHTVVIGGIMEHIEEAGVHSGDSSCVLPPVSLSPAV
                     IETIRDYTKRLALALKVVGLMNVQYAIQRDMVYVLEVNPRASRTVPYVSKATGVPLAK
                     VAARLMAGKKLADMDLPLVHHNGVAEIAVRDFYSVKSPVFPFNKFRGVDTILGPEMRS
                     TGEVMGISTSYGQAFAKAQLAAGQRLPRKGTVFLSVNDRDKKHLVTIGKELSSLGFQL
                     TATRGTTAALRAAGIEAEAVFKVNEGRPNIVDLIKTGKIDLVINTPLGRESFYDEKSI
                     RRAAIRYNIPCITTLAAAQAAARGIRALIEQGTEVAALQDLHRSQVAASGSPASGD"
     gene            3360..4256
                     /locus_tag="VFR42_02785"
     CDS             3360..4256
                     /locus_tag="VFR42_02785"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0368107.1"
                     /translation="MEISGIFPALTTPYGEDGSIAVERFKENISRYNRVGLAGYVVLG
                     STGESVLLSSAESEALLAAAQEVAASGKLMLAGTGAESTAETIARCKRAAALGYDLAL
                     VKTPYYYKPAYKPEVYLRHYRSVADASPIPVLLYSVPIFTGITLETPEIVALAEHPNI
                     VGIKDSSGAIQRVVEVVAQAPSHFHVLTGGAGVIYPALAAGAKGAILALASALPERCV
                     AVYEDCQKGRHAEALAQQQQLARASKTIASDNGIAGLKYAMDLRGYFGGVPRLPLLPL
                     SDEKKQQIHKLVTELEPAAVSA"
     gene            4341..>5903
                     /gene="bshC"
                     /locus_tag="VFR42_02790"
     CDS             4341..>5903
                     /gene="bshC"
                     /locus_tag="VFR42_02790"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEU0368108.1"
                     /translation="MHCRALPIDVLPHQPKLLRDYLQSHERVSAFFQHQPDLENILKV
                     AGALDYPDARRAEVAAVLRQQNELLGAAPATFANLSRFEKGAVAVVSGQQVGLFSGPG
                     YAVYKAIAAARIAKDLTEQGVDAVPVFWMATEDHDLDEVRRTTFFNDGKLIEFELPQG
                     AGNGAPVGRVPLGAAVEALQQAAVTLLGGEQGELAGLLNECYRAEETYGSGFGKLFAR
                     LLKHAGLILLDPLDLRLHRLAQPLFSRAVEERDAIDEALLQRDQQLEQAGYAPQVKVT
                     SRSTVLFHISRDGRQVITDGEGKFQSGERSWNKQELLDAVRSEPENFSPNALFRPVVQ
                     DFLLPTVAYIGGPSEISYFAQSEVLYRHLLGRMPVMLPRPGFTLIDAKAQRLLKQYNI
                     DVEQVWEGPQELGKRMSAASIPENLATLLQENGAEIQKRLKQWSDAVAVLDPTLKTAV
                     ETAQNKIEYQAEKLQQMIGRALDRKSGTVAAHQEFLSNLIYPRKSLQSREVCFLPFAA
                     RWGQAAFEEIERH"
BASE COUNT         1255 a         1662 c         1821 g         1165 t
ORIGIN      
        1 cggagtttac ggatttgatg aaggaatgga agcaaccggg gagttcgcgg cgataactgg
       61 ctcgcgtctg agccgcgtca agtttttcga agaggtgaaa tgccgaagcg gaaagacatc
      121 aagaagattc tgatcatcgg atcgggaccg atcgtcatcg gtcaagcgtg cgagtttgac
      181 tattcgggcg tgcaggcgtg taaatctctg cgcgacgaag gctaccagac cgtgctggtc
      241 aattcgaatc cggcgacgat catgaccgat cccgggatgg cgaaccggac ttatatcgag
      301 ccgcttgcga aggagtacct ggaagcgatc atcgcgcagg agcggcccga tgcgctgctg
      361 ccgaccgtcg gtgggcagac agccttgaac cttgccgtgg aattagccga aggcggcatc
      421 cttgaaaaat acaaagtgga aatgatcggc gcgtcgctgc gcgcaatcaa agtcgccgaa
      481 gaccgtctgt ggtttaagga tgcttgccgc aagattggac tggaggttcc ggcgtccgcg
      541 ctgatcaaca acgcgcagga tgccatgcgc ttgtgcgaac aacttggatt tccgctggtc
      601 atccggccgt cgttcacgct gggcgggacc ggtggagcaa tcgcctataa ccgcgaggag
      661 tttggcgaag cgatccagca tgcgctggac atgagtccgg tgcacgaggc gctggtggaa
      721 gaatcagtgc tcggctggaa agagtacgaa ctcgaggtca tgcgggatta ccgcgacaac
      781 ttcgtggtga tctgcaccat tgaaaatttt gaccccatgg gcgtgcacac cggcgattcg
      841 attaccgtgg cgccggcgca gacgctgacc gacaaggaat accagcggat gcgcgacgca
      901 gcgtcggcga tcttgcgcga agtcggtgtg gaaaccggcg ggtccaacgt gcaattcgcg
      961 gtgaatccgg aaaacggccg catggtggtc atcgagatga atccgcgggt gtcacgcagt
     1021 tcggcactgg caagcaaagc taccggtttt ccgattgcaa agatcgcggc gcggctggca
     1081 gtgggcatgt cgctggacga aattcccaac gatattacga aaaagacccc ggcatcgttt
     1141 gagccgacca tcgattacgt ggtgacaaag attcccaagt ggcaatttga aaagtttccg
     1201 ggctccgaca gcacgctggg cacgcagatg aagtcggtgg gcgaagtgat ggcgattgga
     1261 cgcaccttca aggaatcact ccagaaggcg attcgttcgc tcgaaccgca cactccatgg
     1321 cgcgcgccag ccgacacgcc ggattcgctg ttacgcgaga aactcgctac gccgcggccg
     1381 gatcgcatcc actggcttct aaccgcggtt gagcgcggac tgcacgctga agagatcggc
     1441 gaactgacga agattgatcc gtggtttttg aaacaggtgg aagacctggt ggcgatgaac
     1501 aagcgcgctg ccggcgtcac cgtagaaacc gcttcgaagg aattgttgcg cgaggtgaag
     1561 cgccacggta ccagcgatga gcagctggcg cagatgtgga agaccactcc ggcggcagtg
     1621 cggctacagc gcgcgcgtta cggcgttgcg cctgtgttca agcgcgtcga cacctgcgcg
     1681 gcggaattcg agtcctacac gccatacatg tattcgactt acgaggacga agacgagtcc
     1741 gacgcgacga gcaagccaaa gattatgatt cttggcagtg ggccgaaccg gatcgggcag
     1801 ggaatcgagt tcgactactg ctgctgccat gcttcgttcg cgctgcgcga agacggcttc
     1861 gaaaccatca tggtgaattg caatcctgaa acggtttcca ccgattatga cacctccgat
     1921 cgtctgtatt tcgagccgct gacgctggaa gacgtgctcg caatcgtcga gcgagaaaaa
     1981 ccgcagggcg tcatcgtgca attcggcggc caaacgccgc taaacctcgc gttggaactg
     2041 aaacgcaatg gtgtgccgat catcggcacg gcaccggaat ccattgacct tgcagaagat
     2101 cgtcgtcggt ttggacgcct gctggaagaa ctgaagattc cgcaaccgcg caatgctacg
     2161 gccctggtgc cggaagaagc ggctcgcctg gccggcgaaa ttgggttgcc ggtgctggtg
     2221 cggccaagct atgtgctggg cgggcgtgcg atggtcatcg cctacgacgc gaacacggta
     2281 caggactacg tggcgcaagc ggcgctgatg gggccggcgc gtcctgttct catcgatcag
     2341 ttcctggaag aagccaccga agtggatgtc gacgcgctcg ctgatggcca caccgtcgtc
     2401 atcggcggga tcatggaaca catcgaagaa gctggtgtgc actccggcga ttcttcctgt
     2461 gtccttccgc ccgttagtct ttcgccagcg gtgatcgaaa cgatccgcga ttacacgaaa
     2521 cgtctggcgc tagcactgaa ggtggtcggc ctgatgaacg tgcagtatgc gatacagcgt
     2581 gacatggtct atgtgctgga agtaaacccg cgcgcctcgc gcaccgtgcc atacgtcagc
     2641 aaagccactg gggtgccact ggcaaaagtt gcggctcggt tgatggctgg gaagaaactt
     2701 gccgatatgg atctgccgtt agttcatcac aacggggtgg cggaaattgc cgtgcgcgac
     2761 ttttattcag tcaagtcgcc ggtctttccg tttaacaagt ttcgtggagt cgatacgatc
     2821 ctcgggcccg aaatgcgatc aacgggcgaa gtcatgggca tttccacctc gtacggtcaa
     2881 gccttcgcga aagcgcagct tgccgcgggg cagcgtttgc cgcgcaaagg aacggtgttc
     2941 ctcagcgtga acgaccgaga caaaaagcac ttggtcacga ttggcaagga attgtcttcg
     3001 cttggctttc aactgaccgc cacgcggggt accactgcgg ctttgcgcgc tgccggcatc
     3061 gaagcggagg ccgtattcaa ggtcaacgaa ggccgtccga atatcgtcga cctcatcaag
     3121 accggcaaga ttgatctggt cattaacacg ccgcttggtc gcgagtcgtt ttatgacgaa
     3181 aaatcgattc ggcgggcagc gattcgctac aacattccgt gcatcaccac actggcggca
     3241 gcccaggcgg cggcacgcgg catccgggcg ctgatcgagc agggaaccga agtcgccgca
     3301 ttgcaggatc tgcatcgcag ccaggtcgca gcctcgggct cgcccgcgtc gggagattaa
     3361 tggaaatttc tggaatattc cctgcgctca ccacgcctta cggcgaagac ggctctatcg
     3421 ccgtagaacg atttaaggaa aatatttcgc gctacaacag ggttggcctg gccgggtatg
     3481 tagtcctcgg ctcgaccgga gaatccgtgc tgctttccag cgccgagagc gaggcgcttc
     3541 tcgcggcggc gcaagaagta gctgccagcg gcaaattgat gttagcgggt accggcgcgg
     3601 agtcgacggc ggagaccatc gcccggtgca aacgcgcggc cgccctgggt tatgacctgg
     3661 cgctggtcaa gacgccttac tactacaagc cggcctataa gcccgaagtt taccttcggc
     3721 attatcgcag cgtggccgac gcttcgccca tccctgtact gctttattcc gtgccgattt
     3781 ttaccggcat cacgctcgaa acgcccgaga ttgtggcgct cgctgagcat cccaacatcg
     3841 tcgggatcaa ggacagctcc ggcgcgattc agcgcgtggt ggaagtcgtc gctcaggcgc
     3901 ctagccactt ccacgtgctg accggaggtg ccggggtgat ttatccggcg ctggcggccg
     3961 gtgccaaagg agccattctt gccctcgcgt cggctttgcc cgagcgatgt gtggccgttt
     4021 atgaagattg ccagaaaggg cggcacgcgg aagcgctggc gcagcagcag caactggcgc
     4081 gggcatctaa aaccatcgcc agcgacaacg gaattgccgg tctgaaatat gccatggacc
     4141 tgcgcggcta ctttggcggc gttccgcggc tgcctttgct gccgctttcc gacgaaaaga
     4201 aacaacaaat tcacaaactg gtgaccgaac tggagcctgc cgctgtttca gcctgagcct
     4261 aaagccagtg ccggctttcc ttcacggttc tcccgcgtcc attgcttgtg accgaatcgc
     4321 ccgcgtatca tctaaaatcc atgcattgtc gcgccctgcc gatagacgtc ctgccacacc
     4381 agcccaaatt gctgcgagac tacctgcaat cccacgagcg tgtgagcgcg ttttttcagc
     4441 accagccgga tctggagaac atcctcaagg tagctggtgc actcgactat cccgatgcgc
     4501 gtcgggctga agttgccgca gtcctgcgcc agcagaacga acttctcggc gctgctcccg
     4561 ccacatttgc caatctcagc cgctttgaaa agggtgcggt tgccgtggtt tcgggccagc
     4621 aagtcggact cttcagtggg ccggggtatg ccgtttacaa ggccatcgcc gccgcccgaa
     4681 tcgcaaagga tctgaccgag caaggggtag atgcggttcc tgttttctgg atggctaccg
     4741 aggatcacga tctggacgaa gttcggcgca ccacgttttt caatgacggc aaattgattg
     4801 agttcgaact gccgcaaggc gcggggaacg gcgcgccggt tggcagagtg cctctcggag
     4861 ccgcagtcga agccctgcag caagccgccg tcacgttgct aggcggagag cagggcgaac
     4921 tcgccggcct cctcaacgag tgttatcgcg ctgaggaaac ctacggcagc gggtttggca
     4981 aacttttcgc acggctgctg aagcatgcag gcctgatctt gctcgacccg ctcgatttgc
     5041 gcctgcatcg cctggcgcaa ccgcttttct cgcgagccgt cgaggagcgc gatgcaattg
     5101 acgaagctct cctgcagcgt gaccagcagt tggaacaggc tggctatgcc ccgcaagtaa
     5161 aagtcacttc gcgcagcaca gtgttgttcc acatttcgcg cgacggccgc caggtgatta
     5221 ccgacggaga gggcaaattc caatcgggcg agcggagctg gaataagcag gaactgctcg
     5281 atgccgtgcg gagcgaaccg gagaatttta gccccaacgc gctttttcgg ccggtggtgc
     5341 aggactttct cctgcctacg gttgcctaca ttggcggtcc ctctgaaatt tcgtatttcg
     5401 cgcaatccga agttttgtac cggcatctgc tcggcaggat gccggtgatg cttccgcgac
     5461 cgggctttac cctgatcgat gcgaaagcgc agcgcctctt gaagcagtac aacatcgacg
     5521 tggagcaagt ctgggaaggc ccgcaagaac ttggcaagcg catgagcgct gcgagcattc
     5581 cggagaacct ggccacgctg ctgcaggaaa atggagcgga aatccagaag cgcctgaagc
     5641 agtggtcaga cgcggtggcg gtattggatc cgacgttgaa gaccgccgtt gaaacggcgc
     5701 aaaacaagat cgaataccag gccgaaaagc tgcagcagat gattggccgc gcgttggatc
     5761 gcaagtctgg aacggttgcc gcgcatcagg aattcctgtc caatctgatt tatccccgca
     5821 agtcattgca gtcccgcgag gtttgtttcc tgccttttgc ggcgcgttgg ggccaggcag
     5881 ccttcgagga aatcgagcgg cat
//
LOCUS       DASTPM010000159         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1101373, whole genome shotgun sequence.
ACCESSION   DASTPM010000159 DASTPM010000000
VERSION     DASTPM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101373"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFR42_02795"
     CDS             complement(<1..281)
                     /locus_tag="VFR42_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368109.1"
                     /translation="MKHAALWVILLTMPVRSASVPHNRASPAGQFADNAEVRSALSWF
                     TRNLAWINDQQIRLTEIPAPPFQEAQRAVAVKALLASSGLDVQIDKTGNV"
     gene            complement(353..1597)
                     /locus_tag="VFR42_02800"
     CDS             complement(353..1597)
                     /locus_tag="VFR42_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEU0368110.1"
                     /translation="MTSPTNNEWIYDWNQASAPLIPPGTRILLNDETLRDGLQNPSVH
                     DPSIEEKIELLHLMEALGIDSLKIGLPGAGPRAVAHSEALAREIATNRMKIRPNCAAR
                     TVEADIRPIAEISQRAGIAIEAATFLGSSPIRRLVEDWTVDHLERTTEKAVKFAVEHN
                     LPSMYVTEDTIRTDPSTVKRLYSTAIRSGARAIVLCDTVGHATPQGAYNLIKFAIDEV
                     VKPSGEKIRVDWHGHNDRGLAIANSLWAVAAGADCVHAAANSLGERVGNTAMELMLVN
                     LRLMGLIDRDLSRLKEYCEKVAKATHTSIPPNYPVIGRDAFRTATGVHAAAIIKAFHK
                     KDAALADAVYSGVPSHLFGLDQVIEIGPMSGKSNVLFWLERHGIPADEALVTRIFDAA
                     KQSPRVLTESEVQALCAGAAHH"
     gene            complement(1594..1839)
                     /locus_tag="VFR42_02805"
     CDS             complement(1594..1839)
                     /locus_tag="VFR42_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368111.1"
                     /translation="MAKGTTIYIELLEEGTPCWRPVTAEHLDGDLYRIIGETPDDEVW
                     PFVQGDVVKCRIRTFQSELPQLVAYEKARRESGGAGK"
BASE COUNT          341 a          549 c          685 g          426 t
ORIGIN      
        1 acgttaccgg tcttatctat ttgcacgtcg aggccggagg aagccagcag cgcttttacg
       61 gccaccgcgc gctgcgcttc ctgaaatggc ggggcgggaa tttccgtgag gcggatttgc
      121 tggtcattaa tccaggcgag attgcgggtg aaccaggaca gggccgagcg gacttccgcg
      181 ttgtcggcaa attgcccggc cggagaggcg cggttgtgcg gaacgctggc ggagcgaacg
      241 ggcatggtca agaggataac ccaaagggcg gcgtgtttca tcgcggagtt ccgggcgcgt
      301 tgcgcggccc ggtttcgcgc ggcagattgt cgggcggtcg aatgttgcgc gctcaatggt
      361 gcgcggcccc ggcgcagagc gcttgcactt cggattcggt gagcacgcgc ggggattgct
      421 tggcagcatc gaagatgcgc gtgaccagcg cttcgtcggc gggaatgccg tggcgctcga
      481 gccagaaaag cacgtttgat tttccgctca tcgggccgat ctcgatgacc tgatcgaggc
      541 cgaaaaggtg cgacggcact ccgctataaa cggcgtcggc cagcgcggca tctttcttgt
      601 gaaaggcttt gatgatggcg gcggcgtgca cgccggtggc ggtgcggaac gcgtcgcggc
      661 cgatcaccgg ataattgggc gggatggagg tgtgcgtggc cttggcaact ttttcacaat
      721 attccttgag gcgcgagagg tcgcggtcga tcaggcccat caggcggagg tttacgagca
      781 taagttccat agcggtattg ccgacgcgct cgccgagcga atttgcagcg gcatggacgc
      841 aatcggcgcc tgcagccact gcccacagcg aattggcgat ggccaggccg cgatcgttgt
      901 ggccatgcca atcgacgcgg attttttcgc cgctgggctt caccacttcg tcgatcgcga
      961 attttattaa attataggcg ccttgcggcg ttgcgtggcc gacggtgtcg cacagcacga
     1021 tggcgcgcgc gccactgcga atggcggtgg agtagaggcg cttgaccgta gaaggatcgg
     1081 tgcgaatggt gtcttctgtg acgtacatcg aagggagatt gtgttccacg gcgaacttca
     1141 cggctttttc cgtggtgcgc tcgagatgat cgactgtcca atcttccacc aggcggcgaa
     1201 tggggctgga cccgagaaag gtggcggctt caatggcgat gcccgcgcgc tgggaaattt
     1261 cggcgatggg gcgaatgtcg gcctcgacgg tgcgcgcggc gcagttgggg cgaatcttca
     1321 tgcgattggt ggcgatttcg cgggccagcg cttcgctgtg cgcgacggcg cgcggaccgg
     1381 cgccgggcag cccgattttg agcgaatcga tgcccagcgc ttccatcagg tgcaggagtt
     1441 cgattttttc ttcgatgctg ggatcgtgca cggagggatt ttgcaggccg tcgcgcaagg
     1501 tttcatcgtt gagcaagatg cgcgtgcctg gcggaatcag cggcgcggag gcttggttcc
     1561 agtcgtagat ccattcgttg tttgtgggag aggtcatttg cctgctccac ccgattcccg
     1621 gcgcgccttc tcataagcca ccaactgggg aagttctgac tggaaggtcc ggattctaca
     1681 tttcacgacg tccccttgca caaacggcca gacttcgtcg tccggcgttt ctccgatgat
     1741 gcgatagagg tctccgtcca agtgttccgc cgttactggg cgccagcaag gcgttccttc
     1801 ttccagcagt tcaatgtaga ttgtcgtccc ttttgccatg gtcaacgtaa tccgtggtcg
     1861 cggaacattg tttttagcgc gccgcgggcg tggcgcaggc gggcggccaa gccgcggcca
     1921 tactcgaatt gcagaaaggc gtcggcggcg cgctccagat cggcgccgta gcgataccac
     1981 cacggttttt cgcgccgcgg c
//
LOCUS       DASTPM010000160         7942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1129912, whole genome shotgun sequence.
ACCESSION   DASTPM010000160 DASTPM010000000
VERSION     DASTPM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7942
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129912"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFR42_02810"
     CDS             <1..630
                     /locus_tag="VFR42_02810"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0368112.1"
                     /translation="LGPEYSDAEIDAVLRSHKFKSAVETKNSQEVERSVAELLAKGSV
                     VARFKGRMEFGARALGNRSILANPAVPGVVKIINEMIKCRDFWMPFAPSVLAERSEKY
                     FYKPKPVEAPYMILTLDSRPEKRDVMAAAIHPYDATGRPQEVYESWNPDYHRLISHFE
                     QLTGEAIILNTSFNLHGEPVVCSPEDALRVFDISGLEHLAIGNFLLSKK"
     gene            677..2194
                     /locus_tag="VFR42_02815"
     CDS             677..2194
                     /locus_tag="VFR42_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU0368113.1"
                     /translation="MNAKENPGMPARSDCAELPSQRSAILGMFGQNTFWLWLDMGALR
                     IGGLLAGFFLIRYFGPHDFGLYSTAMAVGFLVNALSDLGLTRFTARAVSADRREAPPI
                     LAVTFLSTALFATVELIALGVALFRGNTTAAVICAGLLVNNFEGSAILCSAMLNATLR
                     ARLILPGSIVSTLCLIVFATLVIVLHLPVLTFVLLSCGRSFAVFLVRLWQLRDLWPRT
                     TAWAAENLRVTIRSAWPYFTYNITQVSYGRISIVCFGLVASQAWVGIYSAAFVLSDIY
                     PQWSYSASGAVLPLWTRLYEGSRMQELVETRESLLDVIVLISVPIAIGLSVFAPEICS
                     FLGPRYVASASVLRIIAYRALLSVIDGLVGHGFLIAINQVRKRQRSQLIALVVLAVLT
                     LALGRWWGPQGVAIALFVADAVLITQYYGILSKLNLQIRTWFFVPVCAAGGCMALISL
                     ALPAEFSLAGRVIAGVLAYGCALFLLSGNRVLDVIRTVRHCLRGIAPSRAAETAP"
     gene            2191..3612
                     /locus_tag="VFR42_02820"
     CDS             2191..3612
                     /locus_tag="VFR42_02820"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU0368114.1"
                     /translation="MISSYQKSAQQLRRLKWIVAALAIALSFAVAMFVPLLSKVQSRA
                     GQLLATHAIYIPFLLIGGVAAVLFFLKFPEIAVSLFYVVGFFKGDPRLDATPVDLTVT
                     VAVMMLLAMGVRLFFTDSTLHLPRDFVFYVPLLALMFISLTYTPDFAAGLDKTLRFVF
                     LTLMGAISPFLLVDTPEKIRRFLVGLVLGGILMSVNSFFMLGGEDRLTAPSGETTALG
                     FSAGLALVIIWALWFPRMSLFRRILLYPFIAVLMVALVGSGGRFANVGTAVCIGLSIL
                     FYRKLVVDLAIMLGAGIAALPFVRIPAASLDYLASLAHPHQAFGTRTDLMFFGLRTFL
                     DHPLFGVGIQGYRYVTPNPLTYNFPHNLLLELGAELGIFAIITFLALAVCSYRSMFRV
                     LRDAYSENAPLYRTVFLMLILTCMDASVSGEMNNDRLLFFMLSMPFVLERIVWKENTA
                     RSESADILVFPNDPSRLESVYSN"
     gene            3618..4934
                     /locus_tag="VFR42_02825"
     CDS             3618..4934
                     /locus_tag="VFR42_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0368115.1"
                     /translation="MEPSSMKFDLSHFVPPPAALAGAAGVQPALDPGVTIPPAIDKKT
                     ATEPMHEGDGISVCHLSPVKGRLDARTFNMEILPAVKHGVRAALVGPHGTEHHENGLD
                     VVPLTGGRSRLSRFFSAPAIAFFARRQPARIYHLHNPEMIPAGLLLKLLFRKTVVYDT
                     QEDFAAMMLTKTYIPPRLRGFMGKAVHAAERLAANLFDGFMTADSGTLRSLAHSGKSK
                     KLVFYNLPNLEFFATLKPRPKRFDVVYRGGLSERAGTFVLLEALQLLRRRGILARALL
                     FGYTDDQAVRSSILDRVGRMNLTSQVFLHGRVPHSEMADTLAQARISVSPLMAIPKFM
                     HNIPVKVFESWACGLPVISSDLPPIRPFFRDGEFGILVPPGDSQALANAIEDLLKNPS
                     RAQQMGRAGRQAVFERCNNKREIHKLISFYSKVLGGRPHTGAATAN"
     gene            4971..5831
                     /locus_tag="VFR42_02830"
     CDS             4971..5831
                     /locus_tag="VFR42_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU0368116.1"
                     /translation="MEKRLIINADDFGLSHGITDGILITHRQGLLTSTSLMVNQPATE
                     YAVRRAAEAPELGVGIHLNLTEGKPVLAKERVPTLVNAAGEFVGPTEMGRRLLRWEAS
                     PREVEAEFRAQIRQMKSLGLPPTHADSHHRIHMYPAAARAFYKAVTAEGIHRARAPRK
                     RYWPSNGRLGGPHIGPLYRRIAAKSYLEFLQSFVFRGLHLPDAGVTFHPKYGSNPTQL
                     PEAWRSTFEFMPEGAYELWCHPGFLQPGFSESDSLSERRELEISILLDPQLRDIVARR
                     QLRLINFSQL"
     gene            5887..7284
                     /locus_tag="VFR42_02835"
     CDS             5887..7284
                     /locus_tag="VFR42_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368117.1"
                     /translation="MIEAQLRANSAALQRAYDRMPYSVQNLLTSARGWFLTRNRYAPR
                     MFSLLRELRGHEKWTSQQITAFQLQALQKIVRHARATSPFYADYPDLNLRSCADIARY
                     PVLTREAVRESGDRLASRAVQTAKRIKVGTTGTTGASLQVTYSEPVARRHWAFRMRQW
                     SWAGVKTRTPRITFFGSRVVPPERTEPPFWTHNFLERQTLASIFHLSESNASHYIDFL
                     RQNSGKVLEGFPSVLAILADFILKTGEPIPMRAVFADGEPLYPFLRESIEQAFATRAY
                     DLYGNTEYAGLIHECEHGQMHAIPEYAYLEILDAEDRPVGPGEEGYFVWTSFVNDAMP
                     LIRYRIGDRGCWLATGPCSCGRNFPLVVPTITRDSDTLRCPDGRLFSPRALNQCLKGA
                     TSLRFCQLLHERPGHVLIRGVASSPDSYADLMEIQKRLQKVLGQQFFVTATLADAPIV
                     RAGGKIPLIIQHGQS"
     gene            7358..>7942
                     /locus_tag="VFR42_02840"
     CDS             7358..>7942
                     /locus_tag="VFR42_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU0368118.1"
                     /translation="MANLAVFGLGYVGSVSAACFASRGNQVIGVDTNLQKLDLLRQGI
                     PPVAEPGLPELVQSAVRNRKLCVTHDVHEAVRRSDVSFISVGTPSRRNGDVDLSALES
                     VLEEIGAALRDHDKFHTIVLRSTVPPGTTSRFAIPILEEMSGRTAGTGFEIYFNPEFL
                     REGCSIRDFHAPPYIVIGKQKNLANGTLRKVWEEM"
BASE COUNT         1477 a         2390 c         2197 g         1878 t
ORIGIN      
        1 ctagggccgg aatattccga cgcggaaatt gacgctgtgc tgcgcagcca caagttcaaa
       61 agcgctgtcg agacaaagaa ttcgcaggaa gtcgaacgca gtgtcgccga actgctggcc
      121 aaggggagcg tggtagctcg tttcaaaggg cgaatggagt ttggcgcgcg ggcgctcgga
      181 aaccgttcga tcctggctaa ccccgcggta ccgggcgtgg tgaaaatcat caacgagatg
      241 atcaaatgcc gcgacttctg gatgccgttc gcgccttcgg tgctggcgga gcggagcgag
      301 aaatacttct acaagccgaa accggtcgag gcgccgtaca tgattctcac gctggatagc
      361 cggccggaaa aacgcgacgt tatggcggcg gcgattcacc cgtacgatgc gacgggtcgg
      421 ccgcaggaag tgtacgaatc ctggaacccc gattatcaca ggctgattag ccatttcgag
      481 cagctgaccg gtgaagccat catcctgaat acgtcgttta acttgcacgg cgagccggtg
      541 gtgtgttcgc cggaagatgc gctgcgcgtg tttgacattt ccgggctcga gcatttggcc
      601 atcggcaact ttctgctttc gaagaaatag ctgtcagcgc aagtcggcca tcgacggaaa
      661 ctaaatctcc atcactatga atgcaaaaga aaacccgggt atgccggcgc ggagcgattg
      721 tgccgagctg ccgtcgcagc gttccgcgat cctcggcatg ttcgggcaaa acacgttctg
      781 gttgtggctg gacatgggcg cgttgcgaat cggaggactg ctggctggct ttttcctgat
      841 ccgctacttc gggccgcacg actttggtct ttatagcacc gcaatggcgg tcggtttttt
      901 ggtgaatgcg ctcagcgatc tgggcctcac gcgcttcacc gcgcgggccg tttccgctga
      961 tcgacgcgaa gccccgccga ttttggcggt aacgtttctt tccacagcgt tgtttgcgac
     1021 ggttgaactc attgctttgg gcgtagcgct gtttcgtgga aacacaactg cggccgtaat
     1081 ttgcgccggg ttgctggtca acaattttga aggctcggcc attttgtgtt cggccatgct
     1141 gaacgcaacg ctgcgcgcgc gcctcatctt gccaggctcc atcgtaagca cgctgtgcct
     1201 aattgtgttt gcgactttag tgatcgtgct gcacttgccg gtgctgacct ttgtgctttt
     1261 gagttgcggc cgcagcttcg ccgtatttct tgtgcggcta tggcagttgc gggacctctg
     1321 gccacggaca accgcgtggg ccgccgaaaa tctgcgcgta acgattcgct cggcctggcc
     1381 ctatttcacc tacaacatca cgcaagtcag ctacgggcga atttcaattg tctgcttcgg
     1441 gttggtggct tcgcaggcgt gggtcggcat ctactccgct gcgtttgtgc tttccgatat
     1501 ttatccgcag tggagctact ccgccagcgg cgcggttctg ccgctgtgga cgcggctcta
     1561 cgaaggcagc cgcatgcagg aattggtgga aacacgcgaa tcacttctcg acgtgattgt
     1621 tctcatttcc gtgcctatcg ccatcggcct ctctgtattc gcgcccgaaa tttgcagctt
     1681 cctcgggccg cgctacgtcg ccagcgcttc ggtgttgcga attatcgcgt atcgcgcgct
     1741 gctttccgtg attgacgggt tggtcggcca cggatttctg atcgccatca atcaagtccg
     1801 caaacgtcag cgttctcagc tgattgctct agttgtgctt gccgtgctca cgctggcgtt
     1861 agggcgttgg tggggaccgc agggcgttgc gattgcgctc ttcgtggcgg acgccgttct
     1921 cattacccag tattacggga tcctttccaa gctgaacttg cagattcgca catggttttt
     1981 cgttcccgta tgcgccgccg gcggctgtat ggccctgatt tcgctggcgc tgccagctga
     2041 attcagtcta gccggtcgcg tgatcgccgg agtacttgcg tatggctgcg cgctcttcct
     2101 gctttccggc aaccgagtcc tggacgtcat tcgaacggtg cggcattgtc tgcgcggcat
     2161 cgcgcccagt cgggcggcgg aaacagcgcc atgatttcca gctaccaaaa gagcgcgcag
     2221 caactgcggc gccttaaatg gatcgtcgca gcgttggcaa tcgcgttgag ctttgccgtg
     2281 gcgatgtttg tgcctctctt gtcgaaagtg cagtctcgcg ccgggcagtt actggcaacc
     2341 cacgcgattt acattccgtt tcttctgatc ggcggcgtgg cggccgtgct ctttttcttg
     2401 aagtttccgg aaattgccgt gtccctgttt tatgttgtgg gtttcttcaa aggcgatccg
     2461 cgtttggacg cgacgccggt ggacctcacc gtcacggttg ccgtaatgat gttgctggcg
     2521 atgggtgttc ggctgttctt cacggacagc acgttgcatt tgccgcgcga cttcgtcttt
     2581 tacgtgcctc ttttggcgct gatgttcatc agtttgacct acacacccga cttcgccgcc
     2641 ggtttagaca aaacgcttcg ttttgttttt ctcaccttga tgggagcaat ttcaccgttc
     2701 ctcttggtgg acacgcccga aaaaatccgc cgtttcctgg tagggctggt gcttggcggc
     2761 atcctgatgt ccgtaaactc atttttcatg ctgggtggag aagaccggct caccgctccc
     2821 agcggcgaaa caaccgcgct cggtttttcc gcgggtttgg cgctcgtgat tatctgggct
     2881 ttgtggttcc cgcgaatgtc gctgtttcgc cgcatcctgc tttacccctt cattgcggtg
     2941 ctgatggtgg cgctggtagg ctcgggcggc cgctttgcga acgtaggcac cgccgtgtgt
     3001 attggcttga gcattttgtt ctaccgcaaa ctggtcgtgg atcttgccat catgttgggc
     3061 gctggcattg cggctttgcc ctttgttcgg attcccgccg cgtcgctcga ctatctggcg
     3121 tcgctcgctc acccgcatca agcttttgga acgcgcaccg acctgatgtt cttcggtctg
     3181 cgaacctttc tggaccatcc gctgttcgga gtcggcattc agggctaccg ttatgtcacg
     3241 ccgaatccgt tgacctacaa ctttccccac aacttgttgc tcgagctggg cgcggaactc
     3301 gggatcttcg ctatcatcac atttctcgcg cttgcggtgt gttcctaccg ctccatgttt
     3361 cgcgtgctgc gcgacgccta ttcggaaaac gcgccgttat accgcacggt tttcctgatg
     3421 ctgatcctga cgtgcatgga cgcctccgtt tccggcgaaa tgaacaatga ccgcttgctc
     3481 tttttcatgc tgagtatgcc gtttgttttg gagcgcatcg tctggaaaga aaacaccgct
     3541 cgctccgaaa gcgccgacat tctggttttc ccgaacgatc cttcgcgact ggaatctgtg
     3601 tacagcaact aaaaagaatg gaaccgtctt cgatgaaatt cgacctgagc catttcgtac
     3661 ctcctccagc cgcactagcg ggagctgccg gagttcaacc tgctctcgat ccgggcgtga
     3721 caattccgcc agcgattgac aaaaagacgg ccaccgaacc aatgcacgaa ggcgacggca
     3781 tttccgtgtg tcatttgtca ccggtaaaag ggcgcttgga tgctcgcact ttcaacatgg
     3841 agattttgcc ggcggtcaaa cacggcgtca gagccgccct ggtcggtcct cacggcacag
     3901 agcaccacga aaacgggctc gacgttgtgc ctcttaccgg gggccgcagc cggttgagcc
     3961 gctttttttc agcgccagcc attgcatttt tcgccagacg ccagccggcg cgcatctacc
     4021 atctgcacaa tcccgagatg attccagcag gtctgctctt gaaattgctc tttcgaaaaa
     4081 cggtcgtgta cgacacgcaa gaagatttcg ccgccatgat gctcaccaag acctacattc
     4141 caccccgcct gcgcggcttc atgggcaagg cggttcacgc cgctgagcgg ttggccgcga
     4201 acctgttcga cggattcatg accgccgatt ccggaacctt gcgctctctg gcgcattccg
     4261 gaaaaagcaa aaagctggtt ttctataacc tcccgaatct ggagtttttc gcgactctca
     4321 agccgaggcc gaaacgcttc gatgtggttt atcgtggcgg cctctctgaa cgggccggca
     4381 ccttcgtgct tctcgaagcg cttcagttgc ttcgccgccg cggaattctg gcgcgcgctc
     4441 tgctttttgg ctacaccgac gaccaagcgg ttcgctcctc gatcctcgat cgcgtcggtc
     4501 gcatgaatct gacttcgcag gttttcctgc atggacgggt tccccattcg gaaatggcgg
     4561 atacgctcgc gcaagcgcgc atttccgttt cgccgttgat ggccattccc aaattcatgc
     4621 acaacattcc ggtcaaagtt tttgagtcct gggcctgcgg tctcccggtg atcagcagcg
     4681 atctcccgcc gattcggcca ttcttccgtg acggcgagtt cggtatttta gttcctccgg
     4741 gcgactcgca agcgctggcc aacgcgatcg aagacctgct gaagaatccc tcgcgcgcgc
     4801 aacagatggg ccgcgcgggc cgccaggccg ttttcgaacg ctgcaacaac aagcgcgaaa
     4861 tccataagct gatttctttc tattcgaagg tattaggtgg acggccgcac accggcgcgg
     4921 caactgcgaa ttgaccaaag cgccgcaaac ggacaactcg ggaaaacaaa atggaaaaaa
     4981 gactgatcat caacgctgac gatttcggct tgagccacgg cattaccgat ggaatcctga
     5041 tcacgcatcg ccagggcctc ctcaccagca cttctctgat ggtgaaccag cccgcgactg
     5101 aatatgccgt gcgccgcgct gccgaagccc ccgagctcgg cgtcggcatc catctcaacc
     5161 tcaccgaggg aaagcctgtt ctcgcgaaag aaagggttcc tacgctcgtc aacgcggccg
     5221 gcgaatttgt cggacccacg gaaatggggc gcaggctgct acgctgggag gcgtcgccgc
     5281 gcgaagtcga agcggaattc cgcgcgcaaa tccgtcagat gaagtcgctc ggcctaccgc
     5341 cgacccacgc cgactcccac caccgcatcc acatgtaccc ggcggcggcg cgtgcttttt
     5401 acaaagcagt gacggccgaa ggcattcacc gcgcccgcgc tccacgcaaa cggtactggc
     5461 cgtcgaacgg ccgcctgggc ggccctcaca ttggtccgtt gtaccgccgc atcgccgcaa
     5521 aatcctatct tgaatttttg cagtccttcg tttttcgcgg tctgcatctt cccgacgcgg
     5581 gcgtgacatt ccatcccaaa tacggcagca accccacgca attgcccgag gcgtggcggt
     5641 ccaccttcga attcatgccg gaaggcgctt atgaactctg gtgccatccc ggttttctcc
     5701 agcccggctt ttccgaatcg gattcgcttt ccgaacgccg cgaactggaa atctcaattc
     5761 tgctggatcc acaactgagg gacattgtcg cccgccggca gcttcggctt atcaattttt
     5821 cccagctgta aacccaggcc cgatcgacga ctcaaccttt cggccttttt ggaatcagga
     5881 ggttccatga ttgaagctca acttcgcgcc aacagtgccg cgttgcagcg tgcctacgat
     5941 cgtatgccgt attcggtgca aaacctgctg accagcgcgc gcggctggtt tctcacgcga
     6001 aaccgttatg cgccgcgcat gttttctctt ttgcgcgagc tgcgcggcca cgagaagtgg
     6061 acctcccaac aaatcaccgc gttccagctg caagctttgc agaagatcgt tcgccacgcg
     6121 cgcgccacat cgccttttta tgctgattat cccgacctca acctgcgatc ttgcgcggat
     6181 atcgctcgtt acccggttct cacgcgagaa gctgtccgcg aaagcggcga ccgcctcgcg
     6241 tcgcgcgccg tgcagactgc caagcgcatc aaagtcggca ccaccggcac caccggcgcc
     6301 agccttcagg tgacctacag cgaaccggtg gcccgccggc actgggcttt tcgcatgcgc
     6361 caatggtcat gggccggggt caaaacgcgc acgcctcgca tcacgttttt tggtagtcgc
     6421 gtcgtgccac ctgagcgtac cgaaccgcct ttctggacgc acaactttct cgaacgccag
     6481 acgctggcca gcatctttca cctttccgaa tcaaacgcat ctcattacat cgattttctg
     6541 cgccagaatt ccggcaaagt tctggaaggt tttccatcgg tccttgccat ccttgccgat
     6601 ttcattctga aaaccggtga gcccattccc atgcgcgccg ttttcgccga tggagagccg
     6661 ctctatccgt tcctccgaga aagcatcgaa caggccttcg cgacgcgcgc ctacgatctc
     6721 tacggcaaca ccgaatatgc gggcctgata catgaatgcg agcacggaca aatgcacgcg
     6781 attccggaat atgcgtatct ggaaattctg gacgccgaag accgtcccgt gggccccggc
     6841 gaagaaggct atttcgtctg gaccagtttc gtaaatgacg ccatgccgct cattcgctat
     6901 cgcatcggag accgcggctg ctggctggca accggtccat gctcctgcgg acgtaacttc
     6961 cccctggttg tgccgacgat tacacgcgac agcgacacgc tacgctgtcc cgatggccgg
     7021 ctgttttcac cgcgcgccct caatcagtgc ttgaagggcg caacgtctct tcgcttttgt
     7081 cagctcctcc acgagcggcc aggccacgtg ctcattcgcg gcgtcgccag cagcccggat
     7141 tcttacgcgg acttaatgga aatccaaaag cgtttgcaga aagtgttggg gcagcagttc
     7201 ttcgtgaccg caacgcttgc cgatgcgccc atcgtgcgcg ccggcggcaa aattccgctg
     7261 attatccaac acggtcagag ctaaatccgc tgtttgttcc ggtttcattt caatcgccag
     7321 ccgtaaaaaa tcaggcgtgt cctggaggct tgtaaaaatg gcaaatcttg cggtgtttgg
     7381 tcttggttat gtcggaagcg tcagcgcggc gtgcttcgcg tcgcgcggca atcaagtgat
     7441 tggtgtcgat acgaatcttc aaaaactcga tttgctccgt cagggaattc cgccggttgc
     7501 agaaccgggc cttccggaac tggtgcagag cgcagttcgt aatcgcaaac tctgcgtcac
     7561 ccacgatgtt catgaagcgg tgcgccgctc cgatgtttcg ttcatcagtg tcggcactcc
     7621 ttctcgtcgc aatggcgacg tcgatttgtc cgctctggaa tccgtgctgg aggagatcgg
     7681 tgccgcactg cgcgatcacg acaagtttca caccatcgtt ctgcgcagca ccgtcccacc
     7741 cggcacgact agtcgctttg cgattccgat tctcgaggaa atgtccggcc gtactgcggg
     7801 taccggcttt gagatctact tcaatccgga attcttgcgc gaaggctgtt ccatccgaga
     7861 ctttcacgcg cccccgtata tcgttatcgg caaacagaaa aaccttgcca acggaacgct
     7921 gcgtaaagtc tgggaagaaa tg
//
LOCUS       DASTPM010000161         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_63293, whole genome shotgun sequence.
ACCESSION   DASTPM010000161 DASTPM010000000
VERSION     DASTPM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63293"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="VFR42_02845"
     CDS             <1..1438
                     /locus_tag="VFR42_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0368119.1"
                     /translation="DLIFEVAPAERGAILLDGTNGREFNSVFARNRDAGNDRAVRVSR
                     TIAERVLSQGLAILGSDVPGSTGLGAVESLIALQVRSLLCVPLTIYQRTIGCIYLDTI
                     NVADRFNDDHLQLVTAIAGISAVALENARRLQWLEQENQRLTTEITLDRTLVGESPRM
                     KDVFQFLARVAPTDATVLVGGESGTGKELAARAIHRKSPRASKPFVAINCAAIPENLL
                     ESELFGYEKGAFTGAVSQKKGRLEMADGGVLFLDEIGELALSLQVKLLRVLQEREFER
                     VGGNRPIHVDIRLIAATNRDLEEASRSGGFRQDLYFRLNVVSLKMPPLREHKEDIPML
                     VSFLVEKHCNKCGAKPKPLSREALACLQNYDWPGNVRELENVIERALVLGSSEDILPE
                     DLPENLLERDPAPGVGEARYHAAVKDLKKRLIQNALEETHGSITEAAKALGVHPNYLH
                     RLIRNLDLKESKPPAPRGPATKQPGARL"
     gene            complement(1439..2383)
                     /locus_tag="VFR42_02850"
     CDS             complement(1439..2383)
                     /locus_tag="VFR42_02850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0368120.1"
                     /translation="MVLIPCSRRLWFVVVVCLLCRSRLLFAQGTKTSPAQQGTRWSMQ
                     VTPAKIVNGVPVFVEVKPTVAMKSMSGTWLGHDLTFDHGSAKNWFVLAGVSLETRPGA
                     YPLKVEAVTSAGKHVHFEKNLKVGLAKYPKIELKVSKQYTEPNPEQQQEIKQAQDVKH
                     EAFSRISPEREWAGSFSAPVNAQTSDIFGTRRVFNGVTKSVHQGLDFRVGTGTPIMAV
                     NYGTVILARFLYFEGNCVVIDHGQGLLSLYLHLSELKVKEGDRVERGTEIGLSGGTGR
                     ATGPHLHLAVRWQGVYLDPAVLLRMKLPDGPTQNSQLR"
     gene            2403..2930
                     /locus_tag="VFR42_02855"
     CDS             2403..2930
                     /locus_tag="VFR42_02855"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU0368121.1"
                     /translation="MAAARTGHSFLILFLIGWLAIPQTGHADGRLPKEAHADKVRVLK
                     SKRTLELIDHDKILKKYKIALGSQPVGKKEHQGDHKTPEGFYFIDHRNERSQFYRSFH
                     ISYPNAEDRVRAKRAGAAPRGDIMIHGLPNGYPWIGSRHRLRDWTDGCIAVSNEEIDE
                     IWRAVADGTPIEIKP"
BASE COUNT          647 a          846 c          866 g          582 t
ORIGIN      
        1 cgatctgatc ttcgaagtcg ccccggcgga gcgcggagcg atccttctcg acgggacaaa
       61 cgggcgcgaa ttcaattctg tcttcgcacg caatcgggat gcggggaacg accgagctgt
      121 gcgggtaagc cggacgattg ccgagcgggt tctctcgcaa gggctggcaa tcctgggttc
      181 cgatgttccg gggagcaccg gtctgggcgc cgtcgaaagc ctgattgcgc tgcaggtacg
      241 ttctctgttg tgtgtgccgt tgacaatcta ccagcgcacg attggttgca tctatcttga
      301 caccatcaac gtcgccgacc gcttcaacga tgaccatctg caactggtca cggccatcgc
      361 gggcatttcc gcggtcgcgc ttgagaatgc gcgtcggctg caatggctgg agcaggaaaa
      421 tcagaggttg accaccgaaa ttactctcga tcgcaccctt gtcggcgaaa gcccgcgcat
      481 gaaggatgtt ttccagttcc tggcgcgcgt agctccgacc gatgcaacag ttctggtcgg
      541 aggcgagagt ggcacgggta aggaactcgc ggcgcgcgcg atccaccgca agagcccgcg
      601 tgccagcaaa ccgttcgtcg ccatcaattg cgccgccatt cccgaaaact tgctggagag
      661 cgagcttttc gggtacgaga agggtgcatt caccggtgcg gtatcgcaga agaaaggacg
      721 cctggaaatg gccgacggcg gcgtgctgtt tctggatgaa atcggcgaac tcgcgctctc
      781 gttgcaggtc aagctactgc gggttctaca agagcgggag tttgagcgcg tgggcggcaa
      841 ccggccgatt catgtggaca ttcgattgat tgcggcaacc aatcgcgatc tcgaagaagc
      901 atctcgcagc ggagggttcc gtcaggatct gtacttccgc ctgaacgtgg tgagcctgaa
      961 gatgccgccc ctccgcgagc acaaggaaga catcccgatg ctggtgagct tcctggtgga
     1021 aaagcattgc aacaaatgcg gcgcaaaacc caaaccactg tcgcgagaag cgctggcttg
     1081 cctgcagaat tacgattggc ccggaaacgt gcgtgaactg gagaatgtca ttgagcgggc
     1141 gctggtgctg ggctcgtcag aggacatttt gccggaagac ttgccggaaa atcttctgga
     1201 acgcgatccc gcgccgggcg tcggagaggc caggtatcac gccgcggtca aggacctgaa
     1261 gaagcgactg atccaaaatg cgctggaaga aacccacggc agcattacag aagcagccaa
     1321 ggcgttgggg gtccacccca actacctgca ccgcttgatc cgtaatctcg atttgaagga
     1381 atcgaaaccg cccgcgcccc gcgggccggc aaccaagcag cccggagcgc gactctaatc
     1441 agcgcaattg cgagttctgg gtcggcccgt ccggtaactt catgcgcaag agaacagcgg
     1501 ggtctaggta cactccctgc caccgcacag cgagatgcag gtgcgggccg gtcgcccgcc
     1561 cagttccgcc actcaatccg atttccgttc cgcgctccac gcggtctccc tcttttacct
     1621 tgagttccga caaatgcagg tagaggctaa gcaagccttg gccgtgatca atcacgacac
     1681 agttcccttc gaagtagagg aatcgcgcca ggatcactgt cccgtaattc accgccatga
     1741 tcggcgtacc ggtgccgacg cgaaaatcta gtccttgatg aacgctcttg gtcacgccat
     1801 tgaagacccg gcgcgtgccg aaaatgtccg acgtttgcgc gtttacggga gctgagaacg
     1861 atccagccca ctctcgctcg ggagaaatac ggctgaacgc ttcgtgcttg acgtcttgcg
     1921 cctgcttgat ttcctgttgc tgctcaggat tgggctcggt gtactgcttt gataccttga
     1981 gttcgatttt cggatacttg gcgagtccga ctttaagatt cttctcgaag tggacgtgtt
     2041 tgccagcgga agtgaccgct tcgaccttca gcggatacgc tcccggccta gtctcaagac
     2101 tcacgcctgc cagtacgaac cagttctttg cgctgccgtg gtcaaaggtg aggtcgtgcc
     2161 ccaaccaggt gccgctcatg gatttcatgg caaccgtggg tttcacttcc acgaacactg
     2221 gaacaccgtt gacaatcttc gcaggtgtga cttgcatgga ccagcgcgtt ccctgttggg
     2281 ctggcgacgt tttggtaccc tgtgcgaaga gcaatcgcga ccggcagagc aggcacacca
     2341 ccacgacaaa ccataagcgt cggctacagg gaatcagaac catcttcaag agcgtagcac
     2401 cagtggcggc tgcgcgaact ggccactcgt tcctaatctt gttcctcatc ggatggctcg
     2461 ccatcccgca gactggccat gcggatggtc gcctcccgaa ggaggcccat gccgacaagg
     2521 tacgggttct aaaaagcaaa cgaacgctcg aactgatcga tcacgacaag attctaaaga
     2581 agtacaagat cgcgctggga agtcagccgg tcggcaagaa agaacaccaa ggtgatcaca
     2641 agactccgga gggcttctac ttcatcgatc accgcaatga acgcagtcag ttttaccgat
     2701 cgtttcacat ctcatatccg aacgcggaag accgcgtgcg ggccaaaagg gcaggtgcag
     2761 cgccccgtgg cgacatcatg attcacgggc tgcctaacgg ctacccatgg atcggcagcc
     2821 gacatcggct gcgcgattgg actgacggtt gtatcgcggt gagcaacgaa gagatagacg
     2881 aaatctggcg ggccgtggcg gatggaacgc caatcgaaat caaaccgtaa gcccatgttt
     2941 a
//
LOCUS       DASTPM010000162         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_668870, whole genome shotgun sequence.
ACCESSION   DASTPM010000162 DASTPM010000000
VERSION     DASTPM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668870"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFR42_02860"
     CDS             complement(<1..828)
                     /locus_tag="VFR42_02860"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0368122.1"
                     /translation="MRTIGFLPLLAIFYGYTAGGPFGYEDIFSQSGPGMALVFLAFVP
                     FFWSIPISFASAELNSILPVQGGFYRWSRAAFGNFWGFQCGWWNWAGTFLLNSLYGVL
                     VMDYLVHYFPWLTGNAKWAGACLVLCLLSYLNMRGIQVSGRLSVAMLVAVLLPVIWLC
                     VVSLFHWHHDPVLPWIPPGKPFSSVFGVGLALAMWNYAGYEQLSSVTAEMDNPQKTFT
                     RILLWNTPMNILTYILPASLAIAALGNWQDWHTGYIVEASRRIGGEALGVAMLLASII
                     "
     gene            1016..2077
                     /locus_tag="VFR42_02865"
     CDS             1016..2077
                     /locus_tag="VFR42_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HEU0368123.1"
                     /translation="MKRILFFTVLVSFFAFEAQADKPKSLFYLTGDSTSIHSFLAHAD
                     KVDTLVPAWYNANAEGLLSGGPNPLVMQTAKQHHVPVMPIVVNAGFVQAEFHKFLGDP
                     AAQRRMLDALIRACKENGYIGIQFDFENIRWTDRDGLSELVKFSAAAFHKEGLQISIA
                     TVPNAPGGPGSETPFSAWLYENWRGAYDLKAIGEAADLVCLMTYDENTQWTVPGPVAG
                     WNWTVANLEYALQAVPPGKLSLGIPLYGYHWFAGMPVKDTSELGYKANPSAKYISAPD
                     AVDLARTYGAKIEWDAAERTAWCYINRGDMREWIFFTDMRTFKERYDLVRERGLQGFC
                     SWVLGTEDLAIWDLLPSHK"
     gene            2189..>2889
                     /locus_tag="VFR42_02870"
     CDS             2189..>2889
                     /locus_tag="VFR42_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEU0368124.1"
                     /translation="MAEEKLPTRAQDFSEWYNQLVLKAQLADYAPVRGCMIVRPYGWA
                     LWENIQQALDSRFKATGHQNVAFPLLIPKSFIEKEKHHVEGFSPELAVVTIGGGEELA
                     EPLILRPTSETIIGHMWSKWIQSYRDLPVLMNQWNSVVRWELRTKLFLRTLEFYWQEG
                     HTAHATQQEAMDETRQMLDVYADFAVNEAAIPVIPGAKSDAEKFAGADITYSIEAMMG
                     DGKALQAGTSHYLGQ"
BASE COUNT          661 a          775 c          869 g          584 t
ORIGIN      
        1 gatgatcgag gccagcagca tggccacgcc gagcgcttcg ccgccgatcc gccgcgacgc
       61 ttccacgatg tagccggtgt gccagtcctg ccagttgccg agcgctgcga tggccagcga
      121 cgcgggcagg atgtaggtaa ggatgttcat cggcgtgttc cacagtaaga ttctggtgaa
      181 cgttttttgc ggattgtcca tctccgcggt cacgctggaa agctgttcgt atccggcgta
      241 attccacatt gccaacgcca gacccacgcc aaataccgag ctaaaaggct ttccgggcgg
      301 aatccaagga agcactggat cgtgatgcca gtgaaaaaga gaaacgacgc acagccaaat
      361 caccggcagc aagactgcga ccagcatggc gacagagagc cttccggaga cctgaatgcc
      421 gcgcatgttg agataggaaa gaagacaaag caccagacac gcgccagccc atttcgcgtt
      481 gccggtaagc cacggaaagt agtggaccag atagtccatc accaaaacgc cgtaaagact
      541 gttcagcaga aaggtgccgg cccagttcca ccagccgcac tgaaatcccc agaagttgcc
      601 gaacgcggca cggctccagc gatagaaacc gccctgcact ggaagaatgc tgttcagctc
      661 cgccgaagcg aacgaaatcg ggatgctcca gaaaaacggc acaaacgcaa gaaacaccag
      721 cgccatgccg gggccagact gcgaaaaaat atcttcgtag ccaaaggggc cgcccgcggt
      781 gtaaccgtag aaaatcgcca gcagcggcag aaacccaatc gtgcgcactg cggcgccttg
      841 ggaccgtgag gctatgccgt ttgcagacaa gaagttgacc ttggcgaagg atgctacctt
      901 gcaccggcgg tattgcaaag gcgcaacaag gaatttcgac aagacgattt gcgacgcgcg
      961 ccctaccaat atgcgtttgc gctcgttgca gaccatccgt tagactcgcg gtcttatgaa
     1021 acggatcctc tttttcacgg ttttggtttc cttttttgct tttgaggcgc aagccgacaa
     1081 acccaagtcg ttgttttatt tgaccggcga ctcgacttcg atccattcgt ttctcgcgca
     1141 cgcggacaag gtggataccc tggtccccgc ctggtacaac gccaatgccg agggtttgct
     1201 gtctggtggg ccgaatccac tggttatgca aacggccaaa cagcatcacg tgccggtgat
     1261 gccgattgtc gttaacgctg gttttgtcca ggcggagttc cacaaatttc tcggcgatcc
     1321 tgctgcacaa agaagaatgc tggatgcact gattcgtgct tgcaaggaaa acggctacat
     1381 cggcattcaa tttgattttg agaatattcg ctggactgac cgagacggtc tttccgaact
     1441 ggtgaagttc agcgccgcag cttttcacaa ggagggtttg cagatttcga ttgcgaccgt
     1501 gcccaacgcg ccgggcggtc cgggaagtga gactccgttt tcggcctggc tgtatgaaaa
     1561 ctggcgcggc gcttacgacc tgaaagccat cggcgaagcc gccgatctgg tttgtctgat
     1621 gacgtatgac gagaacacgc aatggaccgt gccgggaccg gtcgccggct ggaattggac
     1681 ggtcgcaaat ttggaatacg ctctgcaggc tgtcccgcca ggaaaactct cgttgggaat
     1741 tccgctgtac ggctatcact ggttcgccgg aatgccggtg aaggacacca gtgagttggg
     1801 ttataaagcc aatccgtcgg cgaaatatat ttccgcgccg gatgcggtgg accttgctcg
     1861 cacttacggc gcaaaaatcg aatgggacgc ggcggagcgc acggcgtggt gctacatcaa
     1921 tcgtggcgat atgcgcgagt ggatcttctt cactgacatg cgcaccttca aggaacgcta
     1981 cgacctggtg cgcgagcgcg gcctgcaggg cttctgctcg tgggtccttg gcaccgaaga
     2041 tcttgccatc tgggacctgc tcccctcgca caagtaactg cccgcttgca gcagcattcg
     2101 ggcgttcgtt gacagtagtg gaggggtccg ttacgctttt ttgttcgccg ttgcacactt
     2161 ttcctgacaa gggatgattg ggagtttcat ggccgaagaa aaacttccca caagggccca
     2221 ggacttctcc gagtggtaca accagctggt gctaaaggcg caactggcgg actacgcgcc
     2281 ggtgcgcggc tgcatgatcg tgcggccata cggctgggcg ttgtgggaga acatccagca
     2341 agcgctggac tcgcggttca aggcgaccgg tcaccagaac gtggcttttc ctttgttgat
     2401 tccgaagagc tttatcgaaa aagaaaagca ccacgtggag gggttttcgc cggagctggc
     2461 ggtggtgact atcggcggcg gcgaggagct ggccgagccg ctgattctgc ggccgacttc
     2521 ggaaacgatt atcgggcaca tgtggtcgaa atggattcag tcgtaccgcg atttgccggt
     2581 gctgatgaac cagtggaatt cggtagtgcg ctgggaactg cgcaccaaac tgtttttaag
     2641 gacgctggaa ttctactggc aggaagggca taccgcgcac gccacgcagc aggaagcgat
     2701 ggacgagacg cggcagatgc tggacgttta cgcggatttt gcggtgaacg aagcggcgat
     2761 tccggtgata ccgggagcga aatcggatgc ggagaaattc gccggcgcag acataacgta
     2821 ttcgatcgaa gcgatgatgg gcgacggaaa agctttgcag gcgggaacgt cgcattacct
     2881 ggggcaaaa
//
LOCUS       DASTPM010000163         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1130009, whole genome shotgun sequence.
ACCESSION   DASTPM010000163 DASTPM010000000
VERSION     DASTPM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130009"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFR42_02875"
     CDS             <1..486
                     /locus_tag="VFR42_02875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0368125.1"
                     /translation="IINIASSAIPVAYFSSYATSKAALVRFTETIAGEVKPYDVSTFA
                     VGPGTVRTAMAEHSLRSDDGQKWLPWFKRIFDEGLDVSAERPAQFVLELASGRADNLS
                     GRFLSIWDNLDSLLQNVREIEERHLYSLRVQTLDGRNDGSALTAIRGEAERPLAQEPK
                     P"
     gene            758..>2017
                     /locus_tag="VFR42_02880"
     CDS             758..>2017
                     /locus_tag="VFR42_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU0368126.1"
                     /translation="MMRHGMRTQHVWRIMTCLVALCVAAFATAGQDVPPYRNSRLPVE
                     QRVADLLSRMTLEEKVAQLEGTWQNPAFATDPAKRIVDDKGAFLPEHAAVLLKYGLGE
                     MSRPSEKRGPREMAEFTNAVQKWLKENTRLGIPILFHEECLHGNAAPKGTSFPQAIAL
                     ASTWDPPLLHDVFTSVAAEVRARGAQECLAPVLDLAREPRWGRTEETYGEDPYLVSRL
                     GVAAIEGYQGAGPALDKSHVFATAKHFAVHGQPEGGTNAAPGNYSERVIREYFLKPFE
                     AAVREAHVQAVMPSYNEIDGIPSHSNLKLLTGILRQEWGFTGMVVSDYFGIDELRKLH
                     HVVANKPDAARMALEAGVDIELPFADAYQSLPAQISEGRIAESTVDHAVARMLRAKFL
                     AGLFDDPFTDPAAAEKITNSVEHQQLAL"
BASE COUNT          430 a          584 c          578 g          425 t
ORIGIN      
        1 atcatcaaca ttgcgagcag cgccattcca gtcgcctact tctcaagtta cgccacaagc
       61 aaggctgcgc ttgttcgatt cacagagacg atagcgggag aggtgaagcc ttacgacgtc
      121 agcacgttcg cggttggacc gggaacggtg cgtaccgcga tggccgagca ttccctaaga
      181 tccgacgatg gccaaaagtg gcttccctgg ttcaaacgga ttttcgatga aggcttggac
      241 gtatccgcag agcgtcccgc gcagttcgtt ctggagctgg catcgggccg ggcagacaat
      301 ctgtccggac gttttctttc catctgggat aacctcgatt cactcctgca aaacgtgaga
      361 gaaatcgaag aacgacatct ctattctctt cgcgtgcaaa cgctggatgg ccgaaatgac
      421 ggctccgcac tgaccgccat tcgcggggaa gctgagcggc ccctcgctca agaaccaaaa
      481 ccctgacttt ctgactcccg ggaagaaaac gtcaatagtt gcgatgttcc gactcgattg
      541 aaggccattt ttctgcggct ggctaccgct agtccgcggc gacctgctgt tgttctcgcg
      601 aatactgctg ccggagctgc gcttcatcat tattccttgt gccgcacgct gatcatttcc
      661 aattcaccat acatttgcgc tagattctcc cgcacgacgg tcagcggctc ggcccctcgg
      721 tcgagagctc gttcttttgc ggtcttgagg agtcgatatg atgcggcatg gtatgaggac
      781 acagcatgtt tggcgaatca tgacttgcct ggtggccctc tgtgttgccg cgttcgcgac
      841 agccgggcag gatgtgccgc cgtatcggaa ttcgagattg cctgtggagc agcgcgtggc
      901 ggatctactg tcgcggatga cgctcgaaga gaaagtggcg caactcgagg gcacttggca
      961 aaacccggct tttgccaccg atcccgccaa acgcattgtc gacgacaaag gagcctttct
     1021 ccccgaacat gccgccgtgt tgttgaagta tggcttgggg gagatgtcgc ggccgagcga
     1081 aaaacgcggc ccgcgcgaga tggcggaatt cacaaacgcg gtgcagaagt ggctgaaaga
     1141 aaacacgcgg ttagggattc cgattctctt tcatgaagaa tgcctgcatg ggaacgcagc
     1201 gccaaaggga acttcgttcc cgcaagctat tgcgttggct tccacgtggg atccaccgct
     1261 gctgcacgac gtcttcacat cggttgccgc cgaagttcgc gcgcgcgggg cgcaagagtg
     1321 cctggctccc gtgctggacc ttgctcgtga accacgctgg ggacgtacgg aagagaccta
     1381 cggagaagac ccttacctgg tctcgcgtct gggcgttgcg gcgattgagg gataccaggg
     1441 tgccggtcca gccctggata aatcccatgt ttttgccacc gccaaacact tcgccgttca
     1501 cggccagccg gaaggcggca ccaatgccgc tcccggaaac tattccgaac gcgttatccg
     1561 cgaatatttc ctgaagccct tcgaggccgc tgtccgcgaa gcccatgtac aggcggtaat
     1621 gccctcctac aatgaaatcg acggaattcc ctcgcacagc aatctgaaat tgctcactgg
     1681 aatcttgcgg caagagtggg gctttaccgg gatggtcgtc tccgactatt tcgggatcga
     1741 tgaattgcga aaactgcatc acgttgtcgc caacaagccc gacgccgcaa ggatggcgct
     1801 ggaagcgggg gtggatatcg agttgccttt cgcagacgca tatcaatcgc tgccggcgca
     1861 aatctcggaa ggacgcatcg cggaatccac cgtggatcac gcagtcgcac gtatgctccg
     1921 cgccaaattc ctggccggac tgtttgacga tcccttcacc gatccggccg ccgcggagaa
     1981 aatcaccaac agcgttgagc accaacaatt ggcgcta
//
LOCUS       DASTPM010000164         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_236315, whole genome shotgun sequence.
ACCESSION   DASTPM010000164 DASTPM010000000
VERSION     DASTPM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236315"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="VFR42_02885"
     CDS             complement(<1..1482)
                     /locus_tag="VFR42_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0368127.1"
                     /translation="MEQRLMKPMDKRRWLVRFAAFGLSVAWLVAGAEKFFANDARDGA
                     AATREEDDAQSVAEIREHYTKYEYLIVMRDGAKLFTAVYVPKDASPAKTYPLLIERTP
                     YSIGPYGPDNYPKHLGPAPSFVKDGFIFVYQDVRGRYQSDGTFIEMTPHKDAKKGPKD
                     VDESTDTYDTVDWLIKNVPNNNGKAGIYGISYPGFYVAAGMPDTHPALKACSPQAPVT
                     DLYMGDDAYHNGAFMLAANFGFYVFFKPRAGMEFPPKRWRDFDYGTNDGYDFYLAMGP
                     TSNAKKLYMKDTNFYWDDQVRHPNYDEFWKTRDISAHMNNVHCAVLTVGGLFDAEDLV
                     GPYKIFNAVARMSPETPNKIVEGPWVHGGWAGTKGDHLGPVNFAAENSAYFNDHVLLP
                     FFQQYLKDGLDAKLPTALVFETGTNVWRHYDAWPPKNVQTKALYFHAGGKLSWDAASK
                     DAAVGAFDEYISDPNRPVPFVETQTTGVPQTYMDADQRFASKRP"
     gene            1710..2042
                     /locus_tag="VFR42_02890"
     CDS             1710..2042
                     /locus_tag="VFR42_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU0368128.1"
                     /translation="MIARIVTCTVQPAKLNEFRKILNDQILPLVQAQPGFVENIEALD
                     PNNGQFSCTTLWNSRSDVEKYDNSVFAEVACKMSPMLQGNPKVQTLPVENSSTHRVRA
                     AAASASSR"
     gene            2274..2858
                     /locus_tag="VFR42_02895"
     CDS             2274..2858
                     /locus_tag="VFR42_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEU0368129.1"
                     /translation="MVHPWHDVSPGKEVPRDFQAVIEIPLGSNLKYELDKPTGLLKVD
                     RVIHSAVFYPANYGFIPQTYAEDNDPLDVLVLCQEAVQPLALIKARAIGVMTMVDGGA
                     TDDKIIAVATNDPEFSEYLEARDLPGHRLLVLKRFFQDYKQLEGKQVQVEEIRPAYAA
                     IGIIEQSLARYQKHREELLNNEVIPTHNRRKTDK"
     gene            2929..3195
                     /locus_tag="VFR42_02900"
     CDS             2929..3195
                     /locus_tag="VFR42_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368130.1"
                     /translation="MKKISMWIGVSLGALLLIAFVALSVMAGGPKDALYMVRYALPHM
                     HRGNLKVGDSAPDAKLFALNGAETFHVRQRTGGKPLVLVFGSFT"
     gene            3208..3498
                     /locus_tag="VFR42_02905"
                     /pseudo
     CDS             3208..3498
                     /locus_tag="VFR42_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
BASE COUNT          723 a         1083 c          905 g          787 t
ORIGIN      
        1 cggccgcttc gacgcaaaac gctggtcggc gtccatgtag gtctgcggaa caccggtcgt
       61 ctgcgtttcg acgaacggaa ccggtcggtt cggatcactg atgtattcgt cgaacgcacc
      121 aaccgccgca tccttcgacg ccgcgtccca ggatagtttg ccacccgcgt ggaagtacag
      181 cgccttggtc tgcacattct ttggcggcca ggcatcgtaa tgccgccaga cgttcgttcc
      241 tgtttcgaac accagcgcag tcggcagttt ggcgtccaga ccatctttca aatactgctg
      301 gaaaaatggc agcaggacgt ggtcgttgaa ataggccgag ttctcggcgg cgaaatttac
      361 cggccccaaa tgatcgcctt tggtgccggc ccagccgccg tgtacccacg gcccttcgac
      421 aattttattt ggcgtttcgg gactcatcct ggctaccgcg ttgaaaatct tgtacggccc
      481 gaccaaatct tccgcatcga acaaaccgcc gaccgtcagc accgcacagt gaacgttgtt
      541 catgtgagcc gaaatgtcgc gggtcttcca gaattcgtcg taattgggat gccgcacctg
      601 gtcgtcccaa tagaaattgg tgtccttcat gtacagcttc ttggcattcg aagtcggtcc
      661 catcgccaag tagaagtcgt agccgtcgtt cgttccatag tcaaagtcac gccagcgttt
      721 gggcggaaat tccatcccgg cgcgcggttt gaaaaacaca taaaatccaa aattcgcggc
      781 cagcatgaag gcgccgttgt gatacgcatc gtcacccatg tacaagtcgg tgaccggagc
      841 ttgcggcgaa caggccttta acgccggatg ggtgtcaggc atcccggccg ctacgtaaaa
      901 tccgggatac gagatgccgt aaatgccggc cttgccgttg ttgttcggaa cattcttgat
      961 cagccaatcg accgtgtcgt aggtgtctgt actttcgtcg acgtccttcg gccctttctt
     1021 cgcgtccttg tgaggcgtca tctcgatgaa cgttccatca ctctggtagc gtccgcgcac
     1081 atcctgatag acaaaaatga atccgtcctt cacgaagctc ggcgccgggc ccagatgctt
     1141 cggatagttg tccggcccgt acgggccgat gctgtagggg gtgcgctcga tcagcaacgg
     1201 ataggttttc gctggcgagg cgtccttggg cacgtagacg gcggtaaaca gcttcgcgcc
     1261 gtcgcgcatc acaattaggt attcgtattt cgtgtagtgc tcgcggatct cggcgacact
     1321 ttgagcgtca tcttcttccc ttgtggctgc cgcgccgtct cgcgcatcat tcgcgaaaaa
     1381 cttttctgcg ccggcgacta accacgccac gctcagccca aaggccgcaa accgcaccaa
     1441 ccaccgtctc ttatccatcg gcttcatcaa ccgctgctcc attggaattc gctaatctgc
     1501 aaacgctttg gacgatatcg ttcgcgttcc tgactgtcaa cgcactgggc tggttgccgt
     1561 tacagatttg ttccttcttg tccctattgt ctaacgtcct tattgtcttg accttcgcag
     1621 ttcgcggcgc taccctccgc tcttacccgc cccccacgga gctttgccct tctcgcgcag
     1681 ctccttcatt tatccttcgg aggtacatca tgatcgctcg catcgttacg tgcaccgttc
     1741 agcccgcaaa actgaatgag ttccgcaaga ttctcaacga ccagatcttg ccactggttc
     1801 aagcacagcc cggtttcgtg gaaaatatcg aggcgctcga ccccaacaac ggtcaattca
     1861 gctgcacgac gttatggaat tcccggtcag acgtggaaaa gtatgacaac agcgtttttg
     1921 cggaagtcgc ttgcaagatg agcccgatgc tgcagggcaa tccaaaggtg cagacgctgc
     1981 cagtcgagaa ttcttccaca caccgagtgc gggctgcggc agcttcggcg tcaagcagat
     2041 aagccgcgcc tatcccgcac acggaagggc ggagcctcgc tctttccacc acgtggagcg
     2101 ccttcccggc tccgcccttc ctgatatcca ttgttcgtcc ccggctttca ccacaagcgc
     2161 gaaaaggcta ccgctagact cgacatgcgc gaattgcgcc gagcctgcgc atccttctgg
     2221 ccgtcgcatg ctttataatc tttgtttact caaccgctcg cgaggccgct gacatggtgc
     2281 atccctggca tgacgtttcc cccggcaagg aggtcccccg cgacttccag gccgttattg
     2341 agattccgct cggttccaac cttaaatacg aactcgacaa gcccaccgga ctgctaaagg
     2401 tcgatcgagt cattcactcg gccgtttttt atcctgcgaa ttacggtttc atcccgcaga
     2461 cctacgccga ggacaatgat cccctcgacg tgctggttct ttgccaggaa gcggtccagc
     2521 cactcgcact catcaaagcg cgcgccatcg gagtgatgac catggtagac ggaggcgcca
     2581 ccgacgataa aatcatcgcc gtggctacca acgaccccga gttcagcgag tatctggaag
     2641 cccgcgatct tcccggccat cgcctgctgg tgctcaagag gttcttccag gattacaaac
     2701 agctcgaagg caagcaggtg caggtggaag aaattcgccc tgcctacgcc gccattggca
     2761 tcattgagca atcgctggcg cgctaccaaa agcaccggga agagctgctg aacaacgaag
     2821 tgattccgac gcacaaccgc cgcaagaccg acaagtagta gaaaagcatt attccgcgtg
     2881 ggcgtgatgg ttgatacttt gtgcacacct ttttcaagga acctcgcggt gaaaaagatc
     2941 tccatgtgga ttggcgtcag cctgggagcg ctgcttctga ttgcgttcgt tgcgctctcc
     3001 gtgatggcgg gcggacccaa ggacgcgctc tacatggtcc gctacgcgct gccccacatg
     3061 caccgcggaa atctgaaagt gggcgattcc gcccccgatg ccaaactatt cgcgctcaac
     3121 ggcgccgaaa cttttcacgt tcgccagcgc accggcggca aacctctggt tctcgtcttc
     3181 ggcagcttca cttgaccgcc cttccgtcgc caggtcggcg acatcgtgca gatcttcaac
     3241 gattaccatg atcatgcgga ttttctgacc gtctacgtgc gcgaggcgca tcccaccgac
     3301 gaatggcaga tgaagtccaa catcaaggat gacgtttgct acgcccagcc gcgcaatctt
     3361 tccgaccgcc tggctatcgc caacgatttc accaaacgtt tcagctaccc ggtccctttc
     3421 ggcgtggacg atatgaacaa cgccgccaac gacgcctatg ccgcgtggcc cgaacggctc
     3481 tatatcatcg atgaaagt
//
LOCUS       DASTPM010000165         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_5733, whole genome shotgun sequence.
ACCESSION   DASTPM010000165 DASTPM010000000
VERSION     DASTPM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5733"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..2039
                     /locus_tag="VFR42_02910"
     CDS             90..2039
                     /locus_tag="VFR42_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEU0368131.1"
                     /translation="MIESDDKKLGMYRNITRRDFLNGVAVGAAGILGESLLPPSTLSA
                     LEPSAAAQDRPGYYPPALTGMRGSHAGSFEAAHAVRDGKSPGKGARAADTKEAYDLVI
                     VGAGISGLAAAYFWRTKNPNARILILDNHDDFGGHAKRNEFRADGRMLLANGGTWAIE
                     SPFPYSKVARGLMDELGIQPAALEAKSYRRNVYKHLSQAVFFDQQTFGADRLVSPAPG
                     GRRGGGSAEEWKSFLERSPLSAAVRKDILRVETEATDYFPGLTSAQKKDKLSRISYKS
                     FLLDVVKVHKNVIPYYQTRTHGLFGVGIDAVPALDCWVLQQPGFKGLNLEPGPHPRMG
                     YTPKGFASPKEPYEFHYPDGNATIARMLVRKMIPAAAPGHDAEDIVTAGFNYAQLDKA
                     DAPVRIRLSSIVVSVNHVEDQASAKQVDVTYVRDGKLAAVHGQKVILACWNMMIPYLC
                     PELPEKQKEALHYGVKVPLVYTSVAIRNWTAFEKLGVHAISSPGMYHHEMNLDLCVDI
                     GGYESPKTPDQPILVRMERTPCLPGLPERDQHRAGRGDLLSAPFETFERSIRDQFARV
                     LGEGGFDPARDITAITVNRWPHGYAYEYNYLFDPEWPAGESPCEIARKPFGRIAIANS
                     DAGAAAYTDGAMDQAWRAVEELQST"
BASE COUNT          482 a          598 c          669 g          438 t
ORIGIN      
        1 agctggtctt cctgcattac tccgcagcgc ccaaaaaatg attcgttgct gtgcgatgac
       61 tcttacattg cacagcgtcc ggagatttta tgatcgaatc cgacgacaaa aagctcggca
      121 tgtatcggaa catcacgcgg cgcgatttcc tgaacggcgt tgcggtaggc gccgccggaa
      181 ttcttggcga gtcgctgctt ccgccatcga ccctgtctgc cttggaacca tctgccgcgg
      241 cgcaggaccg tccgggctac tacccgcccg cgctcaccgg aatgcgcggc agtcatgccg
      301 gttcgtttga agcggcgcat gcggttcgcg acggtaaatc ccccggcaag ggcgcaagag
      361 cagccgatac caaggaagcc tacgatctgg tgatcgtcgg agccggaatc agcggccttg
      421 cagctgcgta tttctggcgg accaagaatc ccaacgcgcg catcctgatt ctggacaatc
      481 acgacgactt cggcggacat gcaaagcgca acgaatttcg cgcggacggc cgcatgctgc
      541 tggccaacgg cggcacctgg gccatcgaat cgcctttccc ttacagcaaa gttgcgcgcg
      601 ggttgatgga cgaactcggt atccagcctg ctgcgctaga agccaagagc tatcgcagga
      661 acgtttacaa acatttgtcg caagcagtct tttttgacca gcagacgttc ggggcggacc
      721 gcctggtttc gccggctccc ggcggacgga ggggcggagg cagcgctgaa gaatggaaaa
      781 gttttctgga gaggtcgcca ctgtcggcgg cggtgcgaaa agatattctg cgcgtggaga
      841 cagaagcgac ggattatttt ccgggcctga cgtccgcaca gaagaaagac aaactttcgc
      901 gcatcagtta caaaagcttt cttctcgacg tagtcaaggt tcataaaaac gtcattcctt
      961 attatcagac gcgcacacac ggcctgttcg gcgtggggat tgatgccgtg ccggcgttgg
     1021 attgttgggt gttgcagcag cccggcttca aaggactgaa cctggagccg gggccgcatc
     1081 cgcgcatggg ctatacgccg aaaggtttcg cgtcgcctaa ggagccttac gaatttcatt
     1141 acccggatgg caacgccaca attgcgcgca tgctggtgag aaagatgatt ccggcggctg
     1201 cgccggggca cgatgcggaa gacatcgtta ccgcaggatt caactatgca cagctcgaca
     1261 aagcggatgc gccggtacga atccggctaa gcagtattgt ggtatcggtg aaccacgtcg
     1321 aagaccaagc tagcgcaaaa caggtggacg taacttatgt gagggacggg aaacttgccg
     1381 ccgtccatgg gcaaaaagtg attttggcat gctggaacat gatgatcccg tatttgtgcc
     1441 cggagcttcc ggagaagcag aaagaagcgc tgcactacgg cgtgaaagtg ccgctggttt
     1501 acacttcggt ggccattcgt aattggacgg catttgaaaa actcggagtg cacgcgattt
     1561 ccagcccggg gatgtaccac cacgagatga atctggattt gtgcgtggat attggcggct
     1621 acgagtcacc aaaaactcct gaccagccga ttctggttcg catggagcgt acgccgtgct
     1681 tgcccggttt gccggaacgg gatcagcacc gggcggggcg gggcgatttg ctctccgcgc
     1741 cgttcgagac atttgaacgc agcattcgcg atcagtttgc gcgtgttctg ggcgagggcg
     1801 gattcgatcc ggcgcgcgac attaccgcaa ttacggtgaa ccgatggccg catggctacg
     1861 cgtacgagta caactacctg ttcgatcctg aatggcctgc cggcgaaagt ccctgcgaaa
     1921 tcgcgcgtaa gccgttcggg cgaatcgcga tcgcgaattc cgatgcgggc gccgcagctt
     1981 acaccgacgg agctatggat caggcctggc gcgcggtgga agagttgcag agcacgtagc
     2041 cttgcccgca ttatgcaaaa aaaaaatcgt cctgagccat gagcgaagaa aatcccaaaa
     2101 actgctctga ccgcgaactc ggaatggatc gaggcattcc gcgccgcgat tttctgaatg
     2161 gcgcggcgat ggctgtgggg tcggcgc
//
LOCUS       DASTPM010000166         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1274277, whole genome shotgun sequence.
ACCESSION   DASTPM010000166 DASTPM010000000
VERSION     DASTPM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274277"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..426
                     /gene="rpmB"
                     /locus_tag="VFR42_02915"
     CDS             190..426
                     /gene="rpmB"
                     /locus_tag="VFR42_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU0368132.1"
                     /translation="MARVCQVTGKGPMVGNNVSHANNKTKRRFLPNLQNRRIYVESEN
                     RWVSLRLSNAGLRVIDKVGIDAVLVDMRARGEKV"
     gene            440..607
                     /gene="rpmG"
                     /locus_tag="VFR42_02920"
     CDS             440..607
                     /gene="rpmG"
                     /locus_tag="VFR42_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU0368133.1"
                     /translation="MAKTGRDKIKLESTAGTGHFYTTTKNKRTMPAKMEIIKFDPKAR
                     KHVAYKETKIK"
     gene            complement(678..1088)
                     /locus_tag="VFR42_02925"
     CDS             complement(678..1088)
                     /locus_tag="VFR42_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368134.1"
                     /translation="MKFSTQQILRAAVVLALGAASASSFAQSTEYRRGYDAGYGDGVR
                     AAQGGGGRGGDRWNLRIEEATYGVRGASCDARRAVREEAERNRNDVRVGNQLCGDPAR
                     GAQKRLFVTYRCGDGDIMRASGREDEVLHLSCRR"
     gene            complement(1347..2558)
                     /locus_tag="VFR42_02930"
     CDS             complement(1347..2558)
                     /locus_tag="VFR42_02930"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEU0368135.1"
                     /translation="MNLLSSVFDAVLGFLDGGIVGLSGWQVLFYTLAVTHITIASVTI
                     YLHRHQAHRALELHAIPSHFFRFWLWLTTGQVTKEWAAIHRKHHAKCDTEEDPHSPVT
                     RGIKKVFFEGAELYRAESKNLETMEKYGHGTPTDWIERNLYTRFSWQGVVLQLVLNVA
                     LFGVIGITIWAVQMMWIPVMAAGVINGIGHWWGYRNYDCADAATNIIPFGILIGGEEL
                     HNNHHTFATSAKLSNKWYEFDIGWLYIRILETFGLAKVKKVAPEPKFAKDKLVADFDT
                     LQSVIANRYDVMAKYAKSVKLACREEFELLKHKSDLQKPFLKTSRKLLQREPGKLGAP
                     QQEHLVELFQHSKALETMHHMRVELGAIWERSHSTREQLLHQLQDWCVRAEKSGIKSL
                     EEFSLRLRSYA"
     gene            complement(2665..>3270)
                     /locus_tag="VFR42_02935"
     CDS             complement(2665..>3270)
                     /locus_tag="VFR42_02935"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU0368136.1"
                     /translation="VFGGALGVGLGLGMQKIASNYVSGFVILLERSLSIGDMISVDKY
                     SGKVSQINTRYTVLMGLDGVETVLPNEMLISGPVQNQSLSNRTVRVSTVLTAAYGSDL
                     DFLIPMLEALPLGVARVLESPAPGVSLNKFSPDGFELELGFWISDPESGRGSVISEVN
                     KKIYALVQSGDIKLALPALDPRLIDAHIASAVAKNSQSVTN"
BASE COUNT          660 a          971 c          964 g          675 t
ORIGIN      
        1 agaagaaaaa ttgtatgttc gatctcacat tgatggcgcg cgcgcgggca tggattcgcg
       61 ccaagaaaga atgtgacttt tatgaataga caaaagacac aattgttttt cgcaagtgat
      121 tgattccgcg atggttttca cgctatactc tcgtttttcc gaattcggaa gtttatttag
      181 gagtgcgcta tggcacgtgt ttgccaagta actggcaagg gtccgatggt tggcaacaac
      241 gtctcccacg ctaacaacaa aacgaaacgt cgtttcctgc cgaacctgca gaaccgccgt
      301 atctacgtcg aatccgaaaa ccgctgggtc tccctgcgtc tgtccaacgc cggtctgcgc
      361 gtcatcgaca aggttggcat cgacgccgtc ctggtcgaca tgcgcgcccg tggcgaaaaa
      421 gtttaattag ggagctatca tggcaaaaac tggccgcgac aaaatcaagc tggaatcgac
      481 cgcaggcacg ggtcacttct acacgaccac caagaacaag cgcaccatgc ctgcaaaaat
      541 ggagatcatc aagtttgatc cgaaagcacg caagcacgtc gcctacaaag aaaccaagat
      601 caagtaatcg atctttgttc caatgaaaaa agccgctcac tgtcgtgtgc ggcttttttt
      661 atgggcgctg cgatggctta acggcggcag ctcaggtgca ggacctcgtc ctcgcggccg
      721 ctggcgcgca tgatgtcgcc gtcgccgcag cggtaggtca cgaacaaacg cttctgggcg
      781 ccgcgggcgg gatcgccgca cagctggtta ccgacgcgca cgtcgttgcg gttgcgctca
      841 gcttcttcac gcacggcgcg gcgcgcatcg caggacgcgc cacgcacgcc gtaggtggct
      901 tcttcgatgc gcagattcca gcgatcgccg ccgcggccac cgccgccttg cgctgcgcgc
      961 acgccatcgc cgtagccggc gtcatagcca cgacggtatt cggtgctctg tgcaaacgag
     1021 cttgccgagg cggcgcccaa ggccaggaca acggcggcgc gcaagatctg ttgggtcgaa
     1081 aatttcatgg ttattctcct gtcaagttca gtatggaaac atcgtaccgc cacgcgggcc
     1141 gtttgtttgt acgtgtgttc acactgtgca tgtactgaca agaattcgcg ccccaattcc
     1201 tgtaagaagc gtttcaatct gtaagtcggc ctggcggcgc cgcgtgaatt gcctgcatga
     1261 cgttcgatcg tcctcgctgc gatcaaccgg aggccccatg caaaacggct cccgaaggag
     1321 ccgtccagga tgacgcgacg cggacgttac gcgtagctgc gcagccgcag cgaaaactct
     1381 tccagcgact tgatgccgga cttctcggcc cgaacgcacc agtcttgcag ttggtgcagc
     1441 aactgctcgc gggtcgaatg cgaacgctcc cagatcgcgc ccaactcgac acgcatatgg
     1501 tgcatggttt ccagcgcctt gctgtgctgg aacagctcaa ccagatgctc ctgctgcggc
     1561 gcccccagct tgcctggctc gcgctgcagc agcttgcgcg aggtcttcag gaacggcttt
     1621 tgcaagtcgc tcttgtgctt gagcagctcg aactcttcgc ggcacgccag cttgaccgac
     1681 ttggcatact tggccatgac gtcataccgg ttggcgatca ccgactgcag cgtgtcgaag
     1741 tccgcgacca gcttgtcctt ggcgaacttt ggctcaggcg ccactttctt gaccttggcc
     1801 aggccgaagg tttcgaggat gcggatatac agccagccaa tgtcgaactc gtaccacttg
     1861 ttcgacagct tggccgaggt ggcgaaggtg tggtggttgt tgtgcagctc ttcgccgccg
     1921 atcaggatgc cgaacggaat gatgttggtc gctgcgtccg cgcagtcgta gttgcggtac
     1981 ccccaccagt ggccgatgcc gttgatgacg ccggctgcca tgaccgggat ccacatcatc
     2041 tggacggccc agatggtgat gccgatgacg ccgaacaggg cgacgttgag gaccagctgc
     2101 aagaccacgc cctgccagct gaagcgggtg tacaggttgc gctcgatcca gtcggtcggg
     2161 gtgccgtggc cgtacttctc catggtctcc aggttcttcg actcggcgcg gtacagctcg
     2221 gcgccttcga agaacacttt cttgatgccg cgcgtgactg ggctgtgcgg atcctcttcg
     2281 gtatcgcact tggcgtgatg cttgcggtgg atcgcagccc attccttggt gacctggccg
     2341 gtggtgagcc acagccagaa acggaagaag tgactcggaa tggcgtgcag ctccagcgcg
     2401 cggtgcgcct ggtgacggtg caggtagatc gttacgctgg cgatcgtaat gtgcgtcact
     2461 gcaagggtgt agaagaggac ttgccagccg gacaggccga cgatgccgcc gtcgagaaag
     2521 cccaacacgg cgtcaaacac gctgcttaac aaattcattc atgctccaaa tgtggatatt
     2581 gtctacgcct ccgggattct tcgacccggt cagccaacct cacattttac gcggttccct
     2641 gccgcacggc acaattaacc cccatcaatt ggtgacagat tgtgagtttt tcgcgactgc
     2701 cgatgctata tgcgcatcga tcaggcgcgg atcgagcgcc ggcagtgcaa gcttgatatc
     2761 gccggactga accagtgcgt agattttctt gttgacctcc gagatcacgc tgccgcgtcc
     2821 gctttccgga tcgctgatcc aaaagccgag ctcgagctcg aagccgtccg ggctgaactt
     2881 gttgagcgaa acgccaggcg ccggcgattc cagcacgcgc gccacgccca acgggagggc
     2941 ttcgagcatg ggaatcagga aatcgaggtc gctgccgtag gccgcggtca gcacggtcga
     3001 cacccgcacg gtgcggttgc tgagcgactg gttctgcacc ggcccggaaa tgagcatctc
     3061 gttcggcaac accgtctcga cgccgtcgag ccccatcaac acggtgtagc gggtgttgat
     3121 ctggctgacc tttcccgaat acttgtcgac cgagatcatg tcgccgatcg acaggctgcg
     3181 ctcgagcagg atgacgaagc cggacacgta gttgctggcg attttctgca tgccgaggcc
     3241 gaggccgacg ccgagcgcgc cgccgaacac
//
LOCUS       DASTPM010000167         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_986130, whole genome shotgun sequence.
ACCESSION   DASTPM010000167 DASTPM010000000
VERSION     DASTPM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986130"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /gene="aspS"
                     /locus_tag="VFR42_02940"
     CDS             <1..1581
                     /gene="aspS"
                     /locus_tag="VFR42_02940"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU0368137.1"
                     /translation="HARSEFVVAVEGEVVKRQKANPELATGEVEMNATRLHILNNAKT
                     PPFPIEDQINAAEETRLRFRYLDLRRPKPHGNLALRHKIILEIRKTMDDLGFIEVETP
                     MLTRSTPEGARDYLVPSRVHHGHFYALPQSPQIFKQILMIGGLDRYFQIVKCFRDEDL
                     RADRQPEFTQLDLEMSFPRQEDIFHVIEQVMVRACAVAGIKTEAPFPHMLYKDVIRKY
                     GSDKPDMRFGMELHEVTSCFPEEAKQKLQIEGNVFAFAAPSAASYSRKQLDELTEKAK
                     SHGARGAYFVKVAAEGLTSTVEKLIGGENAKRLAEACSAKTGDLIVAVSAKEQIKGTE
                     AAALVAGQLRLQLGEALGAIDKSQWKFLWITGFPLFEWSESDKTWVSAQHPFTGIVDE
                     DLEQLESAPWGVRSKGYDLVLNGYELGSGSIRIHRQDIQERLFKALGLSEEQLRKRFG
                     FFLDALTYGTPPHGGIALGIDRVVMLLAGEKSIREVIAFGKTTAAQDLMAESPSEVDA
                     AQLDQLGIALKQKEKAAE"
     gene            1618..1884
                     /locus_tag="VFR42_02945"
     CDS             1618..1884
                     /locus_tag="VFR42_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368138.1"
                     /translation="MSWLLEVIKSILGLTFHLARSATLRDESRPRWVNYSLAGCSYAF
                     PVLLIVFSFISWKIAIVVAAAFIVSLVIGAVTEADDVWGSQGVE"
     gene            1881..>2308
                     /gene="mutL"
                     /locus_tag="VFR42_02950"
     CDS             1881..>2308
                     /gene="mutL"
                     /locus_tag="VFR42_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU0368139.1"
                     /translation="MSRIRILPESVANKIAAGEVVERPASVVKELLENAIDARAKTIR
                     IETEAGGKRMIRVIDDGHGMTHDDALLAFERHATSKLKTADDLLSIATLGFRGEALPT
                     IASVSRLLLESRDPSEAEGTRVEFANGKLVGVKPAGLPAGT"
BASE COUNT          509 a          718 c          633 g          448 t
ORIGIN      
        1 cacgcgcgca gcgagttcgt ggtcgccgtc gaaggcgaag tggtgaaacg ccagaaagcc
       61 aatcccgagc tcgccaccgg cgaagtcgaa atgaacgcga cgagattgca cattctgaac
      121 aacgcaaaaa cgccgccatt cccgatcgaa gaccagatta acgccgcgga agaaactcgt
      181 ctgcgtttcc gctatctcga cctgcgccgt cccaagccgc atggcaatct cgccctgcgc
      241 cacaagatca ttctggaaat tcgcaagact atggacgacc tgggcttcat cgaagtcgaa
      301 acgcccatgc tcacgcgctc cacgcccgaa ggcgcgcgcg actacctcgt gcccagccgc
      361 gtgcatcatg gccatttcta tgcgctgccg cagtccccgc aaatcttcaa gcagattctg
      421 atgatcggcg gcctcgaccg gtatttccag attgtgaagt gttttcgtga tgaagatctg
      481 cgcgccgacc gccagcccga attcacgcag ctcgacctcg aaatgtcctt cccgcgccaa
      541 gaggacattt ttcacgtcat cgaacaggtc atggtgcgcg cctgtgccgt cgccggaatc
      601 aaaacggaag ccccgtttcc gcacatgctc tacaaggacg tgattcgcaa gtacggcagc
      661 gacaagcccg acatgcgttt tggcatggag ctgcacgaag tcacgtcgtg ctttcctgaa
      721 gaagccaaac agaagctgca gatcgaaggc aacgtcttcg cgttcgccgc tccgagcgct
      781 gccagctatt cgcgcaagca actcgatgaa ctgaccgaaa aagccaagtc ccacggcgcg
      841 cgcggcgcgt acttcgttaa agtcgccgcc gaaggcctta cgtcgaccgt cgaaaaattg
      901 atcggcggag aaaatgccaa gcgactagcc gaggcctgta gcgcaaagac cggtgacttg
      961 atcgttgcag tgtccgctaa agaacagatc aaaggcacag aagcggccgc gctcgtcgcc
     1021 ggccagcttc gtctgcaact cggcgaagcg ttaggcgcca tcgacaaatc gcagtggaaa
     1081 tttctctgga tcacgggctt cccgctcttt gagtggagcg agtccgacaa gacctgggtc
     1141 agcgcgcagc atccgtttac cggcatcgtg gacgaagacc tggaacaact cgaatccgcg
     1201 ccgtggggag tgcgctccaa gggctacgac ctcgttctta acggctacga actcggctcc
     1261 ggcagcatcc ggattcaccg ccaggatatt caggagcggc ttttcaaggc cctcggcctc
     1321 agcgaagagc agctgcgcaa acgcttcggc ttcttcctcg acgccctcac ctacggcacc
     1381 ccgccgcatg gcggcatcgc cctcggcatc gaccgcgtcg tcatgctcct cgccggcgaa
     1441 aaatcgatcc gcgaagtcat cgccttcggc aaaaccactg ccgcccaaga tttaatggcc
     1501 gagtcgccat ccgaagtcga cgccgcccag ctcgaccagc tcggcatcgc cctaaaacaa
     1561 aaggagaagg cggcagagta atatgcttgc ttacaagttt gcgttggaag agcgccgatg
     1621 tcctggttat tagaagtcat aaaatctatt ttgggcctga cctttcatct tgcgcgctcg
     1681 gcaactctac gcgacgaatc tagaccacga tgggtcaact actccttggc gggctgctct
     1741 tacgcgtttc cggtgttgct gatagtcttt tctttcattt cttggaagat cgcgatcgtc
     1801 gttgcagctg cattcatcgt ttccttggtt attggggcgg tgacggaagc agacgatgta
     1861 tgggggtcac aaggagttga atgagccgaa tccgcatact ccccgaatct gtcgccaaca
     1921 aaatcgccgc cggcgaagtt gtcgagcgtc ccgcttccgt ggtcaaggaa ttgctggaaa
     1981 acgcgattga tgcccgcgcg aaaaccattc gcattgaaac cgaagcaggc ggcaagcgca
     2041 tgatccgcgt cattgacgat ggccacggca tgacgcacga cgacgcgctg ctcgccttcg
     2101 aacggcacgc caccagcaag ctcaaaaccg ccgatgacct gctctcgatt gccacgctcg
     2161 ggtttcgcgg cgaagcgctc cctaccatcg cgtccgtttc gcgtctgctg ctggagtccc
     2221 gggacccgtc tgaagcggaa ggcacgcgag tggagttcgc caacggcaaa cttgttggcg
     2281 ttaagcctgc cggtcttccc gctggcac
//
LOCUS       DASTPM010000168         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_611472, whole genome shotgun sequence.
ACCESSION   DASTPM010000168 DASTPM010000000
VERSION     DASTPM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611472"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFR42_02955"
     CDS             <1..400
                     /locus_tag="VFR42_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0368140.1"
                     /translation="SPEQLAEAQTITEIVCVQNLYNLAHRNDDGFINDLAGQGIAYVP
                     FFPLGGFTPLQSAKLDSVAASLESTPMQVALAWLLQRSPNILLIPGTSSVVHLRQNLS
                     AATLTLSSELIDDLNSIGQPAKQVGSAEGA"
     gene            509..970
                     /locus_tag="VFR42_02960"
     CDS             509..970
                     /locus_tag="VFR42_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368141.1"
                     /translation="MKLKVIFATLIVCLAAAAVFAADAFTGTWKLNEAKSKLGAGAVK
                     NTTVTYEVMGDTIKVTLEGVGADGKATHDEWTGKYDGKDYPVTGNPMTDTRAYKKVND
                     HTLEATGKKGDKVVLTAKITVSADGKTRTVVSTQTTADGKKVTSTAVYEKQ"
     gene            1090..>2387
                     /gene="miaB"
                     /locus_tag="VFR42_02965"
     CDS             1090..>2387
                     /gene="miaB"
                     /locus_tag="VFR42_02965"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU0368142.1"
                     /translation="MVKLQTMGSSFDIRLESLTGVPAATPSATLHAMSSLTASSGGRG
                     TFFLETFGCQMNEHDSEKVAGVLVARGYRQVETPEAASLILYNTCSIREKAAQKVFSR
                     LGVYRDTLSKEPGKMQKIGVLGCVAQQEGEAIFSRAPWVSLVCGSASYRNLPQMLARL
                     EAGEERITGLDNDTDETFETEVTRRDNPWRAYLTIIEGCDKACAYCVVPFTRGPERSR
                     DSARVLAELRSLAGMGYSEVQFLGQTVNSYADPSPRKTRFSELLVEAAQVPGIRRVRF
                     TTSHPRDFTPDIVEAIDAHPAICNHVHLPVQSGSTRVLRAMQRTYSRAEYLEKIAMIR
                     GAKRAIAITSDIIVGFPGETETDFDETLSLLDAVKYEGVFSFKYSPRPNTPASSMPDA
                     IPEDEKSRRLAVLMERQRGIQVEALSKLGNEIFEVHVDGKS"
BASE COUNT          525 a          727 c          696 g          439 t
ORIGIN      
        1 cagtcccgag cagttggcgg aggctcagac aattacagag atagtctgcg tgcagaacct
       61 ctacaacctg gcacaccgga acgatgatgg attcatcaat gacctcgcgg ggcaaggcat
      121 agcctatgtg ccgttcttcc cgctcggagg attcacccca ctacagtccg cgaaactcga
      181 tagcgttgcc gcgtcgctag agagcacccc gatgcaggtc gccctcgcat ggctcctcca
      241 gcgctcgccg aatattctgc tgattcccgg cacttcatcc gtcgtgcacc ttcgtcaaaa
      301 tcttagcgcc gcaaccctga cactttccag cgaactcatc gatgatctga actccatcgg
      361 tcaacccgcc aaacaagtgg ggagcgccga gggagcatga cccgtacgga gaaacacttc
      421 ggccgatgag agttgctaac ttcgtccccg ggcgatagaa tccgccgcaa ttgggcccac
      481 cgctagtttt tgctccgagg aggagtgcat gaaattgaaa gtcatttttg ccacgctgat
      541 tgtgtgcctg gcggctgccg cggttttcgc ggccgacgcg ttcaccggca cgtggaaatt
      601 gaatgaagcc aaatccaaac taggtgcggg agcagtcaaa aacactaccg tcacctacga
      661 ggttatgggc gacaccatca aggtcacact cgaaggtgtg ggcgccgatg gcaaggccac
      721 gcacgacgaa tggacgggca agtacgacgg caaagactat cccgttaccg gcaatccaat
      781 gaccgatacg cgggcctaca agaaggtgaa tgaccacacg ctggaagcta ccgggaagaa
      841 gggcgacaag gtggtgctga ccgccaagat caccgtttcc gctgacggaa agacacgcac
      901 cgtcgtctca acccaaacta ccgccgacgg caagaaagtg accagcaccg ccgtctacga
      961 aaagcagtaa cggctccgca gcttctacca tgccgccgcc aagcgcgccc gttcgctggg
     1021 cgtatgcttc cggcggcatt ttcctttccc gctggtccat cctcgtacga acagccagcc
     1081 gcggttcgta tggtaaaatt gcaaaccatg ggcagcagct tcgacattcg actggaatcg
     1141 ctcaccggtg tgccggcagc caccccgtcg gcgacgctgc atgcgatgtc ttcgctgact
     1201 gcatcgtcgg gcggtcgcgg cactttcttc ctcgaaacct ttggctgcca gatgaacgag
     1261 cacgactcag aaaaagtcgc cggggtgctg gtggcccgcg gttaccggca ggtggagacg
     1321 ccggaagcgg cgagcttgat tctgtacaac acgtgcagca tccgcgaaaa ggcggcgcaa
     1381 aaagttttct cgcgtctcgg cgtgtaccgc gacacgctga gcaaagagcc cggcaaaatg
     1441 caaaaaatcg gcgtactcgg ctgcgtggcg cagcaggagg gggaagccat cttctcccgc
     1501 gcgccgtggg tgagcctggt ctgcggttcg gccagctacc gcaaccttcc gcagatgctg
     1561 gcgcgtctcg aagccggcga agagcgcatc accggcctcg acaacgacac cgacgaaacg
     1621 tttgaaacgg aagtcacccg ccgcgacaat ccctggcgcg cctacctcac cattatcgaa
     1681 ggctgcgaca aggcttgcgc ttattgcgtg gtgcctttca cgcgcggccc ggaacgaagc
     1741 cgcgattccg ctcgcgtgct cgccgaattg cgatctctcg ctggcatggg ctactccgaa
     1801 gtgcaattcc tcgggcaaac ggtcaactcc tacgccgacc cttcgccgcg caaaacgcgc
     1861 ttcagcgaat tgctcgtcga agccgcgcag gttccgggga ttcgccgtgt gcggtttacc
     1921 acttcgcatc cgcgcgactt cacgccggat attgtggagg cgatcgacgc gcatccggcc
     1981 atttgcaacc acgtccatct gccggtgcaa tccgggtcca cgcgagtttt gcgcgcgatg
     2041 cagcggacgt attcgcgcgc ggagtatctg gaaaagattg cgatgatccg cggcgcgaag
     2101 cgcgcaatcg cgatcacgtc ggacattatt gtcggatttc ccggcgaaac ggaaacggac
     2161 ttcgacgaaa cgctttcctt actcgacgcg gtgaaatacg aaggcgtgtt ttcgttcaag
     2221 tattcgccgc ggccgaacac tccggcaagt tcgatgccgg atgcgattcc ggaagacgag
     2281 aagagccggc ggctggcagt gttgatggaa cggcagcgcg ggatccaagt ggaggcgctg
     2341 agcaagctgg gcaacgagat ttttgaagta cacgtagacg gcaaatc
//
LOCUS       DASTPM010000169         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_842086, whole genome shotgun sequence.
ACCESSION   DASTPM010000169 DASTPM010000000
VERSION     DASTPM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842086"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1202
                     /locus_tag="VFR42_02970"
     CDS             240..1202
                     /locus_tag="VFR42_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0368143.1"
                     /translation="MTKKNLWVAAGVALLAVGLIALPSHSRSMQDPQATPPAPPEAGV
                     REMPDVELTPGMPDIEREFNIVTSLSDDMGSWLGVETREVTAENVKEFKLPGERGVVI
                     GKVLDDSPAAKAGLKDNDVVTEINGQRVEGAAQFRRMIREIPAGRSVQLTVWRNGRAQ
                     TISTTLGKMQEGRKLRISKAAPQVFNFRVPEIAPMPEIRSFEWDGGDLLGSRPRLGID
                     AEDIGGQLGSYFGAPDGEGILVREVNPGSAAEKAGVKAGDVITSLNGDKIHGLAELRS
                     KLAGNDGKPIKLGVLRNKSALTLDVEIPAAKQKTVHKMEMRTKI"
     gene            complement(1291..2448)
                     /locus_tag="VFR42_02975"
     CDS             complement(1291..2448)
                     /locus_tag="VFR42_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU0368144.1"
                     /translation="MSETFNLDQVAGELNSRKSSGSEDPSGTERLDHWLASLVHRYGS
                     DLLLVEGAPPCIRKDGEIRKIENTVLDGADIEAAVIPALSQHALNLFRERGIADSSYR
                     IQGLGRFRINLHRERSRSAAAIRALPTRVPALRELHLPPSVENLARLPRGLVLLGGPA
                     GSGKSTTMAALINEINQREARHIVIIEDPIEYEHTHIRSVVEQVEIGADAPDFPTALR
                     AALRQAPDVIVVGEMRDPETMRIAVAAAETGHLVLSTLHTVDVASTISRISDSFALER
                     QNSIRQELAMALAAVHTQMLLPKKGGGRVPAAELLMVAYGARHHIRRNALQHLHQEIA
                     LTKKKGSFTLEDSLVQLIHSGQIDREEALHCSIHPDDLDILLKSAASGAHA"
BASE COUNT          561 a          699 c          871 g          490 t
ORIGIN      
        1 aaggctcgct gcgaccgcgc gaaatccacg ggaaactggg acagggcgga tcaccgatca
       61 agctgcacac ggtcaacggc ggcatccgcg tggtggcgct gcgcaccact gtctaggatt
      121 ttgagacaac tctttgacaa cgtgtgcgtc taagtgcaca gccgcgcacc gcggtgagtt
      181 ggcaatcgct cgctgcgcat gagtgaaacg gacagcgcgg cgtggtaagg aggagaagaa
      241 tgactaagaa aaatttatgg gtggccgcag gagtagcgct attggcagtg ggactgattg
      301 cgttgcctag ccactcgcgt tcgatgcagg atcctcaagc aactccgccg gcgccacccg
      361 aagccggagt gcgagaaatg ccggatgtgg agcttactcc gggcatgccg gatattgaac
      421 gcgaattcaa cattgtgact tcgctgagcg acgacatggg cagctggctg ggcgtcgaaa
      481 cgcgcgaagt aaccgcggag aacgtgaagg aattcaagct gcctggcgag cgcggcgtgg
      541 tgatcggaaa agtgctggac gacagccccg cggccaaagc cggtttgaaa gacaacgacg
      601 tggtgaccga gattaacggt cagcgcgtcg aaggcgctgc gcagttccgc agaatgattc
      661 gcgaaattcc cgccggccga agcgtgcagc tgaccgtgtg gcgcaatggc cgcgcgcaaa
      721 ccatcagcac cacgctcgga aaaatgcagg aaggccgcaa gcttcgcatt tcgaaggccg
      781 cgccccaggt tttcaacttc cgcgtgccgg aaatcgcgcc gatgccggag attcgctcat
      841 tcgaatggga tgggggagac ctgcttggca gccggccgcg actgggcatt gacgcagaag
      901 acatcggcgg gcagctcggt tcgtattttg gcgcgccgga tggcgaggga attttggtgc
      961 gcgaagtgaa tcccggttcg gctgcggaaa aagccggagt aaaagcggga gacgtaatca
     1021 cttcgctcaa cggcgacaaa atccacggcc tggccgagct gcgctccaag ctggcgggca
     1081 acgacggcaa gcccatcaag ctaggcgtgc tgcggaacaa gagcgcgttg acgctagatg
     1141 tggaaattcc cgcggcgaaa cagaagaccg tacacaagat ggagatgcgg acgaagatct
     1201 gatcgactca atcagagtcc tattaagcaa aggctcttcg ctcgggcgga gggcctttct
     1261 ttttgcgaga tgcttagagc gcttcattcg ctacgcgtgc gcacccgacg ccgcggactt
     1321 cagcagaatg tccaggtcgt cgggatgaat cgagcagtgc agcgcttctt cgcgatcgat
     1381 ttgccccgaa tgaatcagct gaacgagtga atcctccagc gtaaaagaac ctttcttttt
     1441 ggtaagggcg atttcctgat gcaggtgctg gagcgcgttg cggcggatgt gatggcgcgc
     1501 gccgtaggcg accatcagca actcggcagc gggaacgcgc ccaccgccct ttttagggag
     1561 gagcatctgc gtgtgcaccg cggcaagcgc catggccaac tcctgacgga tggagttttg
     1621 cctctccaat gcgaacgaat cggaaatgcg cgagatcgtg gaagcaacgt cgacggtgtg
     1681 cagcgtggag aggaccaggt ggccggtttc cgcggcagcg accgcgatgc gcattgtttc
     1741 gggatcccgc atttcgccga cgacaatcac atcgggcgct tgacgcaatg ccgcgcgcaa
     1801 agcagtggga aaatcgggcg catcggcgcc aatttcaact tgttcgacca cgctgcgaat
     1861 atgcgtgtgt tcatattcga tggggtcttc gatgatgacg atgtgccgcg cttcgcgctg
     1921 attgatttcg ttgattagcg cggccatggt ggtggatttg ccggaaccgg cggggccgcc
     1981 aagcagaacg agaccgcgtg gcaggcgtgc gagattttcg accgatggcg gaagatgcag
     2041 ctcacgcagg gcgggaactc ttgtgggcag cgcgcgaatc gccgcggcgg aacgggagcg
     2101 ttcgcgatgc aggttgatgc ggaagcggcc cagcccctga atgcgataag aggaatcggc
     2161 gatgccgcgt tcacgaaaga gattcaaggc gtgttgcgag agggcaggaa tgaccgcagc
     2221 ctcgatgtcc gcgccgtcga gaacggtgtt ttcgattttg cggatttcgc catccttgcg
     2281 aatacaaggc ggagcgcctt cgaccagcag caaatcgctg ccgtagcgat gcaccaggct
     2341 cgccagccag tggtcgagcc gctcggttcc cgacgggtct tcggagccgg acgatttgcg
     2401 gctgttgagt tcgccggcga cttgatcgag gttgaaagtt tcactcatgg atgactcacg
     2461 cggttggtgg acatgaggtg ccgcaaggca gcagtgtgcc gcatcgaaag gctttcgtcc
     2521 actggcttcg tctgccagca aagttggccg gatgtgggtg attttccata cgcagggcta
     2581 taatcaggcg aattgcggcc gccgaggtgg cgtcgttggt c
//
LOCUS       DASTPM010000170         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1130203, whole genome shotgun sequence.
ACCESSION   DASTPM010000170 DASTPM010000000
VERSION     DASTPM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130203"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFR42_02980"
     CDS             complement(<1..440)
                     /locus_tag="VFR42_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU0368145.1"
                     /translation="MNGSRRLRALFPFCALSVFLACGCASRRTTASRTDSPTTQPEDT
                     SESKEQPNTPERSEPPAETSNRASAARPAKKRDAATPNPALSAYTEEGKASWYGIPFH
                     GRRASNGEIYDMNKMTAAHRTLPFDTVVRVTNLSNGKSAVVRIT"
     gene            complement(437..646)
                     /locus_tag="VFR42_02985"
     CDS             complement(437..646)
                     /locus_tag="VFR42_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368146.1"
                     /translation="MDKFEEFGKRIDEELTRLRRYIETEVAPETERRSAEFLREVSAK
                     LSEAASKLEARNARRTTPPPPNPQS"
     gene            721..1641
                     /locus_tag="VFR42_02990"
     CDS             721..1641
                     /locus_tag="VFR42_02990"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0368147.1"
                     /translation="MPLPYQWQYRLDRWKAALQNMFGGGNQQPRPKLCPNCGSLVGIN
                     ATRCHNCGTNLKFSVAAINKSLSGVFSGPSPVTTALLVSNLLMFGVEFMAIAAQGGGG
                     GLSILWGVGWEATYRLGMSWHDAIYVPHEWYRLITAMFLHGGLIHIGFNMMALMQLGP
                     ALEELYGSSRYFFLYIFTGAFGFLLSSFIGNNSLGASGGLLGLVGAMLAITSKRGGAY
                     MRELRSRLVSNVVILFVLGFMGMGIDNWAHGGGLAAGFFLGKIFADRQPMNVRERQVA
                     NALGWIAGIAVIASFALMLLHYRDPLPGQG"
     gene            complement(1632..>2051)
                     /locus_tag="VFR42_02995"
     CDS             complement(1632..>2051)
                     /locus_tag="VFR42_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0368148.1"
                     /translation="LARKLNQRSSLGAYLDPIADKLLLSSSFVILAMEKQIAWWLAII
                     VLSRDVIILVVAVVIILIWGYRPFPPSLLGKLTTFFQIVLVFTVVLVAAYPNVTLASI
                     KQILIYTVTVFATVSAFHYSFSIARKLSSPAQPPAQP"
BASE COUNT          376 a          536 c          688 g          451 t
ORIGIN      
        1 tcggtgatgc ggactaccgc ggatttgccg ttgcttagat tggttacgcg gaccacggtg
       61 tcaaagggca gagtgcgatg cgccgcggtc attttgttca tgtcgtagat ttcgccgttc
      121 gaagcgcggc gcccgtggaa ggggatgccg taccatgagg ctttgccttc ctcggtgtag
      181 gcggagagcg cgggatttgg tgttgcagcg tcgcgtttct tcgcaggccg cgcggcggaa
      241 gcgcggttgg aagtctctgc gggcggctcg gagcgctcgg gggtgttcgg ttgttctttg
      301 ctttcggaag tatcttcagg ctgcgtggtt ggggagtcag tgcggctcgc ggtggtgcgt
      361 cgggacgcgc aaccgcatgc caggaaaaca ctcagcgcgc agaacgggaa gagcgcgcgg
      421 agacggcgag aaccattcac gattgcgggt tgggcggcgg aggcgtggtg cggcgggcgt
      481 tgcgcgcttc cagcttgctg gccgcttcgc tgagcttggc ggaaacttcg cgcaaaaatt
      541 ccgcgctgcg gcgctcggtt tccggcgcca cttcggtttc gatgtagcgg cgcaggcgcg
      601 tcaattcctc gtcaatgcgt ttgccgaact cttcaaattt gtccatagag gtgatcgccg
      661 gactggccgg ggcgatttgg gtattatagg aacgccgcgc gagcgccgca aggaggaccg
      721 ttgccgcttc cctatcaatg gcagtaccgg ctggaccgct ggaaagctgc gctgcagaac
      781 atgtttggcg gcggaaacca gcagccgcgt cccaagctgt gcccgaactg cggctcgctg
      841 gtgggcatca acgctacgcg ttgccacaat tgcggcacta acctgaaatt ttccgtggcg
      901 gccatcaata agagcctctc tggggttttc tccgggccgt caccggtaac gaccgcgctg
      961 ctggtgtcga atctgctgat gttcggcgtc gaatttatgg ctatcgctgc gcagggcggc
     1021 gggggcggac tgagcatttt atggggtgtc ggctgggagg cgacctatcg cctcgggatg
     1081 agctggcatg acgcgattta cgtgcctcat gaatggtatc ggctgatcac cgcgatgttt
     1141 ctgcacggcg gattgattca catcggattt aacatgatgg cgctgatgca gctgggtccg
     1201 gcgcttgaag agctctacgg ctcctcgcga tatttctttc tgtacatctt caccggcgcg
     1261 ttcggatttc tactgagttc ttttatcggc aacaattcgc tgggggccag cggcggactg
     1321 ctgggcctgg tgggtgcgat gctggcaatc acttcgaaac gcggcggcgc gtacatgcgc
     1381 gagctgcgtt cgcggctggt cagcaacgtg gtgattctgt ttgtgctggg attcatgggc
     1441 atgggcatcg acaattgggc gcacggcggc gggctggctg ccggattttt tcttggcaaa
     1501 atatttgccg accggcagcc gatgaatgtg cgcgaacgcc aggtggccaa tgcgcttggc
     1561 tggattgcgg gaatcgcggt gattgccagt ttcgcgctca tgcttttgca ctaccgcgat
     1621 ccgcttccgg gtcagggctg agccgggggc tgggctggcg agctcagttt ccgcgcgatg
     1681 ctgaaggaat aatggaacgc ggaaacggtg gcgaacacgg tgaccgtgta aatcagaatc
     1741 tgcttgatgc tagccagcgt cacgttggga taagccgcca ccagcaccac tgtgaacacc
     1801 agcacaatct gaaaaaaagt ggtgagcttg cccagcaaac tggggggaaa cggccggtag
     1861 ccccagatca aaataattac taccgccacc accagaataa ttacgtcgcg actcagcaca
     1921 atgatggcca gccaccaggc gatttgtttt tccatcgcca aaatcacaaa ggaagaagag
     1981 agcagcagct tgtccgcgat gggatcgagg taggctccca gtgaagagcg ctgattgagt
     2041 ttgcgggcca g
//
LOCUS       DASTPM010000171         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_149890, whole genome shotgun sequence.
ACCESSION   DASTPM010000171 DASTPM010000000
VERSION     DASTPM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149890"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1982
                     /locus_tag="VFR42_03000"
     CDS             159..1982
                     /locus_tag="VFR42_03000"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368149.1"
                     /translation="MADTSVPGVSSAPLPPERRLESWGEIATYLRREIRTVQRWERNL
                     GLPVHRLSVGKQSSVFAYPSELDKWFRERESRLKEDKDDDTATRSPEIPNRTTSPEDR
                     GERNPDENAEQRRVRRRALWLSGLAFLIVAGVLVARFTVSVQPRVSVAQGDKLRLFVR
                     PFKTIAGDASQAEFTEGLTSEMITRLGHIEPKHLGVIAPTSSQQLGGKPIAELVSLLG
                     VNYVLEGTVRRGNDQVRINMTLVSAKDQTPLWSDSFTENITDILKVQNKVADSVAEKL
                     LLSLPPVDAASPARVDPAGYDYYLRGRRAWMVRDLAHAVSDYKLAVTWLPDYALAHSG
                     LAAAYALLGEAPNDAVPPTESAPKARAEAQRALTLDPSNAEAHYVLGNLAMYFDFDFP
                     AAEREFRRALALEPNNPTAHEWLGQFLAAQNRMAEAQAETLKALELDPVSPIFTTARA
                     DAFYYARDFDSTIQQANLTLEQSPNFVLAEFWLASGQREKKLYPAALKHFRAANHLAP
                     YSPALMMALGHALAVSGDRPGALAVLTQLQTLSTKRHVPAIYFAGVYVGLNDKDMAFH
                     FLDKAFQEHDDRLPYLAVEPMADPLRSDPRFHDLLAKMNLR"
BASE COUNT          457 a          697 c          639 g          498 t
ORIGIN      
        1 ataactccgt ccgctcgcag cctcgaacct ccactccccc tttcggccgc ggatgaaaca
       61 tttgcttcat tttctgtttt tgggttgtgc acagcttggc aatgacggcc gggcgatata
      121 tactccgccc aattccatcg cctggaaaaa ctagtcaaat ggctgacacg tccgtgcccg
      181 gggtgagcag cgcaccgctg ccgccggaac gtcgcctcga gtcctggggc gagatcgcca
      241 cataccttcg ccgcgaaata cgcacggtgc agaggtggga gcggaacctc ggcctgcccg
      301 tccatcgcct ctccgtcggc aaacaatcct cggtttttgc ttatccttcc gaactggaca
      361 aatggtttcg cgaacgcgag tcgcgcctca aggaagacaa ggacgacgat acggctacgc
      421 ggtcgccgga aatcccgaat aggacgacca gtcctgaaga tcgcggggag cggaatcccg
      481 atgaaaacgc cgagcagcgt cgagttcgcc ggagggcgct gtggttgagc ggtcttgcgt
      541 tcttgattgt cgcgggcgta ctggttgcgc gatttacggt cagcgttcaa ccccgggttt
      601 cggttgcgca aggtgacaag cttcgcctct ttgtaaggcc attcaaaaca atcgcgggag
      661 acgcatcaca agctgagttc accgaaggtc tcaccagcga aatgatcacg cggctcggcc
      721 acattgagcc gaagcatctt ggagtaattg cgccgacgtc gtcacaacaa cttggtggca
      781 aaccaatcgc cgaattggtg tctctactcg gggttaacta cgtcttagaa ggaacggttc
      841 gccggggcaa tgatcaggtc cgcatcaata tgacgctggt ttccgcgaag gaccaaacgc
      901 cactgtggtc tgactccttc accgaaaaca taacggacat tctaaaggta caaaacaagg
      961 tagcggattc ggttgctgaa aaactcctgc tcagccttcc gcccgtggat gctgcttcgc
     1021 ctgcgagagt tgaccctgcg ggatatgact actatctgcg cgggcgcagg gcatggatgg
     1081 ttcgcgattt ggcgcacgca gtttccgatt acaaactcgc tgtgacgtgg ctgcccgact
     1141 atgccctggc gcattcgggc ctggcggccg cgtacgcgct gctcggcgaa gcaccgaacg
     1201 acgccgttcc gccgacggaa tccgccccca aggccagggc cgaggcgcaa cgcgctctga
     1261 cgcttgatcc ctccaacgcc gaagcccact acgttctggg caatctggcg atgtatttcg
     1321 actttgattt cccggctgca gaacgggagt tccggcgagc acttgccctc gagccgaaca
     1381 atcccacagc ccacgaatgg ctgggccaat tcctcgcggc gcaaaaccgc atggcggaag
     1441 cacaggcgga aacattgaaa gcgcttgagc tggaccccgt ttctccgatt ttcacgacgg
     1501 cgcgggcgga cgcgttctac tatgcgcgcg attttgattc gaccatccag caggcgaacc
     1561 tcacgctcga acagtctccc aatttcgttc tggcagaatt ctggctggcc tccggccagc
     1621 gcgagaaaaa gttgtacccc gccgctctta agcacttccg tgcggccaac cacctggctc
     1681 cgtacagccc ggcgctgatg atggcattgg gccatgctct tgccgtttca ggcgaccgtc
     1741 ccggagctct cgcagttttg actcagctcc agaccctatc caccaagcgc catgttccgg
     1801 cgatctattt cgccggtgtt tatgtgggat tgaacgacaa agatatggct tttcactttc
     1861 tggataaggc gttccaggag cacgacgacc gtctgccgta tcttgccgtg gagcccatgg
     1921 ccgaccccct ccgctccgat cctcgcttcc atgatctgct tgccaaaatg aaccttcgct
     1981 agctcgcggg cttcttactt ccctgttttg ctccacttgt aggcgggtgc tggctagatt
     2041 gcgtgacctt tgcggcgagc cgagtcttcg gcttggagcg acatccgacg tcagttcccc
     2101 accggcgcac cttgtcgctt gcgtgtcgtt ttgcgctttc cgtagcttcc atgcacggca
     2161 tcgttttcgt ttcgtatgtg tcgaacagag gtcggcaatt cgaaacgaac ggagggagtg
     2221 gcacggtcac ccgctccctc tgatttcaaa ccaacatggt tcgaaagaag aaggagacgt
     2281 tatggcggtc c
//
LOCUS       DASTPM010000172         7523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1072556, whole genome shotgun sequence.
ACCESSION   DASTPM010000172 DASTPM010000000
VERSION     DASTPM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7523
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072556"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFR42_03005"
     CDS             <1..696
                     /locus_tag="VFR42_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368150.1"
                     /translation="DASREVLRVKQPAGTFHCPLNLPRPTQDAKLLLKLLRLRLQDKP
                     PNAPVQKLWIRADTDRPLAVQGGLFLPAAPDPDKLELTLARIASVVGENNVGSAELLD
                     SHRPDAFRMQTFATTGVALRKTVSVASGEIPEELHKAKIGFRYFRPPLPVYVALDEGH
                     PTKVSWKGNTGKVVHASGPWRLSGQWWEEHPWQEDTWEVELSFAGKTPLSGTYQIVFD
                     GLQKKWFVRGSHD"
     gene            696..3845
                     /locus_tag="VFR42_03010"
     CDS             696..3845
                     /locus_tag="VFR42_03010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEU0368151.1"
                     /translation="MYVELHARSAFSFLEGASTPEQLASACSALSMPSMALLDRDGMY
                     GSPRFHLAMTKIGLKAHIGAEISCAEGGRLVLLVSSAEGYKNLCRLLTRMKLRSEKGK
                     GAIRLEEAEEFSSGLICLTGGEEGLFANSLRQGGPKTASALVERLRGIFGRENLYAEL
                     QRHFLREEERRNQVAVELARRFHLPLLATNGVCHASPEEREILDAFSCLRHHRVLANA
                     GKLLSCNAERYLKSPEQMAALFADLPEAIANTNELAARLQYTMRDLGYQFPKYPTPNG
                     ESQIEFLRARAWEGMLNRYGSGNEKAEKQIGKELDLIEKLDLAGYFLIVWDIIRFCRE
                     KNILVQGRGSAANSAVCYALGITAVDAVGMNLLFERFLSEERGEWPDIDLDLPSGDQR
                     ERAIQYVYERYGKFGAAMCANVITYRGKSAAREIGKVLSFDAETLDKLSSLVSAWEYS
                     DSKDTLARQFKDAGLDLGQHRILEFFELCQKVQDLPRHLGQHSGGMVICQGQLDSVVP
                     LEPATMPGRVVVQWDKEDCADMGIVKVDLLGLGMMAVLEDTIRLIREHQQEEIDLSHL
                     PQNDKAVYSALQTADTIGLFQVESRAQMSCLPRLKPTTFYDIVVQVAIIRPGPIVGKM
                     VHPYLNRRQGREPAETLHPLLESTLKRTLGVPLFQEQLLRMAMLAAGFSGGEAEELRR
                     ALGFKRSEARMKEVEVKLRRGMAANGIVGETQEKIVQSITSFALYGFPESHAASFALI
                     AYASAFLKCHYLAAFTAAMLNNQPMGFYRPATLIKDAQRHGLKFKPIDITQSQWDCTI
                     EDHGGELRVRIGLRYVKGLRKDTALQIVEQRAKHPFVSIDDVKRRIPQMQINELRVLA
                     SIGALNFISGKTAVHRRDALWQVERANRRPGPLMENVALPEIPEVAASSGSPLRPMTP
                     QERIFADFRGTGLTVGPHPLAQHRVQLNALGISPAGSLKHLLEGKSVQVVGAVIVRQR
                     PGTAKGFVFLSLEDETGVANIIITPQLFERDHTVVVHHAFLQIRGLVQNQDGVVSVKA
                     VHIEPFELATAEVPSHDFH"
     gene            complement(3858..5186)
                     /locus_tag="VFR42_03015"
     CDS             complement(3858..5186)
                     /locus_tag="VFR42_03015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368152.1"
                     /translation="MLEHVAGNAGTRLGTANQATVRKIGLRLLPFLFLLYIVAFLDRI
                     NVSFAGLEMTRDLNFSDRVFGLGAGIFFVGYVVFEIPGALLVERWSARKVIARILLTW
                     GIVTVLVALVRTPVQFYAARFLLGAAEAGFFPGVLVYLTHWFRYEDRAKAAALFMAAI
                     PVANVIGSPLAGAILNVHWAGWPGWRWLFVLEGLPAVVLGVVTLYFLPDWPVDASWLA
                     REERAWIIRELESEKTAKTVAREISIGRALRMPRVILLTLVYFLSVTGIYGFAMWFPT
                     ILKRATGYSNLTVTLLAALPYVAGVAAMLFNGWHSDRSRERRWHTAIPLFAGAACLGG
                     AVFFSDQLAVAFALMIVVGACTTAFLPSFWPLPSEFLVASAAAAAMGLINALGNLGGF
                     LGPYAMGFFRSRTGSFTPGLLVLLSCMAGAGMLVLLLPARAVAQSDRGIG"
     gene            complement(5246..5815)
                     /locus_tag="VFR42_03020"
     CDS             complement(5246..5815)
                     /locus_tag="VFR42_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368153.1"
                     /translation="MGEVKGEWIEMLGSKNAEERVRAAAEIYHSGGEQAESAVRIWWT
                     NPELVRLCGTPPTVTVGLAVRPTTFARIREANGWPQLAVVPPEQDAAEFELHFAGGVL
                     LDVLTTREESGTGAIARFLSKHGEGVQQVEFRCEDVDRAAAILQEQFGAAAVYPAKRA
                     GADGTKVNFFLVVAPDNSKVLIELYEPER"
     gene            complement(5843..6769)
                     /locus_tag="VFR42_03025"
     CDS             complement(5843..6769)
                     /locus_tag="VFR42_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0368154.1"
                     /translation="MDTPLHWIAFISFVLVAVTLDLGVFHRQAHKVSLKESLAWSVVW
                     IALAVAFGLWILYGYGRQHALEFFTGYVIEKALSVDNLFVFLVVFRVFAVDEKYQHRV
                     LGYGILGALLMRGAMIAAGAALIERFEWIMYVFGAFIIYAGLHMLLAGEAQSHPENNL
                     LVRYVSKHLRLTQEYREEKFFVRESGKLFATPLFLVLLVVEISDVTFAVDSIPAIFGI
                     TRDPFIVFTSNVFAILGLRALYFLLAGVLDKFVYLKVGLGLVLVFVGAKMIIEPWLHI
                     SVAVSLTVVCGMLAVAMIVSLLVKPKAEPATK"
     gene            complement(6874..>7523)
                     /locus_tag="VFR42_03030"
     CDS             complement(6874..>7523)
                     /locus_tag="VFR42_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368155.1"
                     /translation="RDGSPAREKFIDGLKLSPELRAWLQKHDVMDLTLPGVDKLITPD
                     DVLQVPEFLQAYERSNRGGVTSGLPVPKFREADKAGNPARYEKQHQEYMSALKKFITG
                     RPETMSGMELELDGVNPARKWAEAQNTHKRRVLQMAPAEAQTKYLAAKADTDLEGHAS
                     IGSLAPGNYWLSTLALTATAGDARLRWDVPVKIAAGRTLRIELTNLNATDNLSSR"
BASE COUNT         1722 a         2301 c         1953 g         1547 t
ORIGIN      
        1 gacgcctcgc gagaagtttt acgcgtcaag cagcctgctg gaactttcca ttgtccgctt
       61 aatctgccgc ggcctacgca agacgccaaa ctgctgttga aacttctgcg cttgcggctg
      121 caggacaaac cgcctaacgc accggtacaa aaattatgga tacgcgccga tactgatcgg
      181 ccgctcgccg tccagggtgg cttgtttctc ccagctgcac ctgatcctga caagctggag
      241 ctgacactcg ctcgcatcgc cagtgtagta ggcgaaaaca atgtcggctc ggcggaactt
      301 ctagattctc atcgtccgga tgcttttcgg atgcaaacat ttgctacgac gggtgttgct
      361 ttacgaaaaa ctgtgagtgt ggctagcgga gaaattccag aagaactgca caaagcaaaa
      421 atcggttttc gctatttccg cccgccgctg cccgtctacg ttgccctcga tgaagggcat
      481 cccacaaaag tcagctggaa aggaaacacc gggaaggtag tacatgcttc ggggccatgg
      541 cgcttgtccg ggcagtggtg ggaagagcat ccctggcagg aagacacttg ggaagtcgag
      601 ctcagcttcg ccggaaaaac tccgctttcc ggcacttatc aaatcgtctt tgatgggctg
      661 cagaaaaagt ggtttgtgcg tgggagccac gactgatgta tgtcgaactt catgcgcggt
      721 ccgcattcag ctttctggaa ggcgcatcca cgccagagca acttgctagt gcctgttcag
      781 ctttaagcat gccgtctatg gcgttgctgg atcgcgatgg aatgtacggt tcgccgcgct
      841 ttcacctggc catgacaaaa attgggctga aggcgcacat cggcgcggaa atttcttgcg
      901 ccgaaggtgg gcggctggtc ctgcttgtca gctccgcgga aggttacaaa aatctgtgcc
      961 gccttttgac gcgtatgaaa ttgcgctcgg aaaaaggcaa gggcgcaatt cgccttgagg
     1021 aagcggaaga attcagcagt gggctgattt gcctgaccgg cggagaggaa gggctattcg
     1081 caaacagcct tcgtcaaggt ggcccgaaaa ctgcttcagc gctcgtcgaa cggctgcgcg
     1141 gaatttttgg ccgagaaaat ctctacgccg aactgcagcg ccacttcctt cgtgaagaag
     1201 agcgccgcaa ccaggtagcg gtggaactgg cacgacggtt tcaccttcca cttctagcca
     1261 ccaacggcgt ctgccatgcc tcgccggaag agcgcgaaat tctcgacgcc ttctcctgtt
     1321 tgcgccatca tcgcgtgctg gccaacgccg gcaagttgct ctcttgcaat gcggagcgct
     1381 atctcaagtc gcccgaacaa atggcggcac tctttgccga tttgcccgaa gccatcgcca
     1441 acactaacga actcgctgcg cgcctgcagt acaccatgcg cgacctcggc taccagtttc
     1501 caaaatatcc gacgccaaat ggcgaatcgc aaatcgagtt tttgcgcgcg cgcgcttggg
     1561 aaggaatgct gaaccgttat ggctcaggaa acgaaaaggc cgagaagcag atcggcaagg
     1621 agttggacct tatcgaaaaa ctcgaccttg ccggctattt cctgatcgtt tgggacatca
     1681 tccgtttctg ccgcgagaaa aatatcctgg ttcagggtcg gggctccgcc gcgaacagcg
     1741 ccgtgtgcta tgcgttgggc ataactgcgg tcgatgcggt tggcatgaac ttgttgttcg
     1801 agcggttcct atccgaagaa cgcggtgaat ggccggacat tgatctcgat cttcccagcg
     1861 gcgatcagcg cgaacgtgcc atccaatacg tttatgagcg ttacggaaaa tttggcgctg
     1921 cgatgtgcgc caatgtcatc acctatcgtg gaaaatcggc ggctcgtgaa atcggcaaag
     1981 tactgtcctt cgatgcagaa actctcgaca agctttccag cctggtcagc gcctgggaat
     2041 acagcgattc gaaagacacg ctggcgcggc aattcaagga tgccggcctc gatctgggcc
     2101 agcaccgcat ccttgaattt ttcgagttgt gccagaaagt gcaggaccta ccgcgtcacc
     2161 tgggccagca ttccggcgga atggtcattt gtcaggggca gctggactcc gtcgttccgc
     2221 ttgagcccgc cactatgccc ggacgtgtcg tcgtgcaatg ggacaaggaa gattgcgcgg
     2281 acatgggcat cgtcaaagtc gatttgcttg ggctgggcat gatggctgtt ctggaagata
     2341 cgatccggct tattcgagaa caccagcaag aggaaatcga tttaagccat ttgccgcaaa
     2401 acgataaggc cgtttattcg gccctgcaga ccgccgacac cattggttta tttcaagtgg
     2461 aaagccgcgc acaaatgtcc tgcttgccgc ggctcaagcc cacaaccttt tatgacatcg
     2521 tcgtacaggt ggcgattatc cgtcccggcc cgatcgtcgg aaaaatggtt catccctact
     2581 tgaatcgccg tcagggccgc gagcctgccg agacgctgca tcctcttctc gaatccactc
     2641 tcaagcgcac gctcggcgtc cctcttttcc aggaacagct tctccgcatg gccatgcttg
     2701 ccgcgggctt tagcggtggg gaagcggaag aactccgccg cgcgcttggt ttcaaacgat
     2761 cggaagcacg catgaaagaa gtcgaagtca agttgcgccg cggaatggcg gcaaacggca
     2821 ttgttggcga aacgcaggaa aaaatcgttc aatccatcac ttcgttcgct ttgtatggtt
     2881 ttccggaatc ccatgcggcc agcttcgcac taattgcgta cgccagcgcg ttcttgaaat
     2941 gccactacct cgcggcattc accgcggcca tgctcaataa tcagccgatg ggtttctatc
     3001 gaccggcaac tttgatcaaa gacgcacagc gacatggctt gaaattcaag ccgattgaca
     3061 ttactcaatc gcagtgggat tgcacgatcg aagatcacgg cggcgaattg cgtgtgcgca
     3121 tcggtttgcg ttacgtcaag ggtctgcgca aagacactgc gctgcagatt gtggagcagc
     3181 gtgcgaagca tccttttgtt tcaattgacg acgtcaaacg ccgcattccg caaatgcaga
     3241 tcaatgaatt gcgtgtgctg gcttccatcg gcgctcttaa tttcatttcc ggcaaaacag
     3301 ccgtgcatcg ccgcgatgca ctgtggcaag tggagcgcgc caatcgccgc ccagggccgc
     3361 tgatggaaaa tgtcgctctg ccggaaatac cggaagttgc cgcgtcgtcc ggatcgcctt
     3421 tgcgtccgat gaccccccaa gaacgcatct tcgcggactt tcgcggcact ggcctgaccg
     3481 tcggcccgca tcccttagcg caacatcgtg tacaactgaa cgcgctggga atttcaccgg
     3541 caggcagcct gaaacatctt cttgagggaa aatccgttca agttgtcggc gctgtgatag
     3601 ttcgccagcg ccccggcact gccaaaggat ttgtctttct gagtctggaa gacgaaaccg
     3661 gagtggccaa catcatcatc acgccgcagc tcttcgagcg tgatcacacc gtcgtggttc
     3721 atcacgcttt cttgcaaatc aggggacttg tgcaaaatca ggacggcgtt gtttcggtaa
     3781 aagccgtcca catcgagccg ttcgaattag ccaccgccga agtgccttcc cacgactttc
     3841 actaagacaa acttccacta gcctattcct cgatcgcttt gcgcaactgc gcgtgccggc
     3901 aaaagcaaca ccagcatccc cgcacccgcc atgcagctca gcaggaccag cagcccgggc
     3961 gtaaacgaac cagtgcgcga tcggaaaaac cccatcgcgt aaggtcccag aaaaccgccc
     4021 agattgccca gcgcgttaat cagccccatc gccgcagccg ctgccgaggc caccagaaac
     4081 tccgacggca acggccaaaa gcttggcaaa aacgccgtcg tgcaagcacc aaccacaatc
     4141 atcagcgcaa aagccacagc aagctggtcg ctgaagaaaa ccgctccgcc aagacacgca
     4201 gcaccagcaa acaacggaat ggcggtgtgc cagcgccgtt cgcgtgagcg atcggaatgc
     4261 catccgttaa aaagcattgc cgcgacgcct gcaacataag gaagcgccgc gagcaaagta
     4321 accgtcagat tagaatagcc agtggcgcgc ttcagaatcg tcggaaacca catcgcaaag
     4381 ccatagatgc cggtgacgga aagaaagtac accagcgtca acagaatcac tcgcggcatc
     4441 cgcaacgctc ggccgatgga aatttctctc gcgactgttt tcgccgtttt ttcagattca
     4501 agctcccgga taatccacgc gcgttcttcc ctggctagcc agctggcgtc tactggccag
     4561 tcgggaagga aatagagcgt aaccactccc aggacaaccg ccggaagccc ctccagaaca
     4621 aaaagccatc gccagccggg ccatcccgcc cagtgcacgt tcagtattgc tcccgcgagc
     4681 ggcgatccaa tcacgttcgc caccggaatc gccgccataa acaatgccgc ggctttggca
     4741 cggtcctcgt agcggaacca gtgcgtcaga tacaccagca ctcccggaaa gaagcccgcc
     4801 tccgccgcgc ccagcaagaa tctggctgca tagaactgca caggtgtgcg caccaacgcg
     4861 accaataccg tcacaattcc ccaagtcaac aagatgcgcg cgatcacctt acgcgcgctc
     4921 catcgctcca ccagaagcgc accggggatt tcgaacacca catatccaac aaagaaaatt
     4981 cctgctccca gcccaaacac gcgatcgctg aaattcagat cgcgagtcat ctccagcccg
     5041 gcaaaactca cgttgatgcg atcgagaaac gccacgatgt acagcaaaaa gagaaaggga
     5101 agcagtctca ggccgatttt gcgaacggtg gcttgattcg ctgtccctag ccgcgtaccg
     5161 gcatttcctg cgacatgttc cagcacttaa aactccacca cggcggcctc aggaaatctc
     5221 aacggaacca cctggaatga gcgtcttacc gttcgggctc gtacaactcg atcagtactt
     5281 tgctattgtc tggtgccact accagaaaga agttaacctt cgtgccgtct gctccggctc
     5341 tctttgcagg atataccgcc gctgccccaa actgttcttg cagaatcgcc gccgcacggt
     5401 ccacgtcctc gcagcgaaac tccacctgct gcacgccctc gccgtgtttc gataggaacc
     5461 gcgcaatcgc tcccgtgcct gattcctcgc gagtcgtcag tacgtccagc aaaactcctc
     5521 cggcaaaatg aagctcaaat tcagcggcgt cctgctccgg cggcacgacc gccagctgcg
     5581 gccagccatt cgcctcacga atcctggcga atgtcgtcgg ccgaaccgcc agtcccaccg
     5641 tcacggttgg aggtgtgccg catagccgca ccaactccgg attggtccac caaatccgca
     5701 ccgccgattc cgcctgttct cctccgctgt ggtaaatctc agcggcagcg cgaacgcgct
     5761 cttcggcatt cttgctgccg agcatctcaa tccattcgcc ctttacttcg cccatgaacg
     5821 ttccgctatt ctctgcagaa aattattttg tcgcaggctc cgctttcggc ttcaccagca
     5881 gcgacacaat catcgccacg gctaacattc cacaaactac tgtcaacgaa accgccaccg
     5941 aaatgtgcag ccacggttcg atgatcatct tggcgcccac aaaaaccagc accagcccca
     6001 gccctacttt caaataaaca aacttgtcca gcacgccagc cagcaggaaa tacagtgcgc
     6061 gcaatcccag aatagcgaac acgttggagg taaacacgat gaacggatcc cgcgtgatgc
     6121 caaaaatcgc cggaatggag tctaccgcga aggttacatc gctaatttcc accaccagca
     6181 acaccagaaa cagcggagta gcaaacagct tgccgctctc cctcacaaaa aatttctctt
     6241 cgcgatattc ctgcgtcagc cgcaaatgct tgctgacgta gcgcaccaga agattgtttt
     6301 ctggatggct ttgcgcctcc ccggccagca acatatgcag accggcatag atgatgaacg
     6361 ctccgaaaac gtacatgatc cattcgaagc gttcgatcag cgccgcgcct gccgcaatca
     6421 tggccccacg catcagcaat gcgccaagaa ttccatagcc cagcacgcga tgctggtatt
     6481 tctcatccac cgcgaagact cggaagacca caagaaagac aaacagattg tctacgctca
     6541 gagctttttc gatcacatag ccggtgaaaa actccagcgc gtgctgccgc ccgtagccgt
     6601 atagaatcca tagaccgaac gcgactgcca gcgcgatcca caccacgctc cacgccagcg
     6661 attctttcag ggaaaccttg tgcgcctggc ggtgaaacac ccccaggtcc agcgtcacag
     6721 caaccagcac aaacgaaatg aaggcaatcc agtgaagcgg tgtgtccatc tccccgtcag
     6781 ttcttccaga tcatggctct gccgctttgg tcgagaagcc ggcggcgctt gccgttctgc
     6841 gcggagcaca cgctgtctcc gcgcgctctt ttttcagcgt gaagatagat tgtcggtcgc
     6901 gttcagattc gtcaactcaa ttcggagggt ccgtccggcg gcgattttca ccggcacgtc
     6961 ccagcgcagc cgcgcatctc cggcggtcgc ggtcagcgcg agagtactca gccaataatt
     7021 tccgggtgcc aggctgccaa tcgaagcgtg cccctccaga tcggtgtcgg ctttcgccgc
     7081 caggtacttg gtctgagctt ctgcgggcgc catttgcaga acccgtcgct tatgagtgtt
     7141 ttgtgcttcc gcccatttgc gtgcggggtt cacgccgtcc aactccagct ccatgccgga
     7201 catggtttcc ggccgccccg taatgaattt cttcaacgcg ctcatgtact cctggtgctg
     7261 cttttcgtaa cgtgccggat ttccggcctt gtcggcctct ctgaattttg gcaccggcag
     7321 gccgctggtc actccccctc tgttcgaacg ttcgtaggcc tgcaagaatt ccggcacttg
     7381 cagcacgtca tccggcgtga tgagcttgtc gactccaggc agcgtaaggt ccatcacgtc
     7441 gtgcttttgc agccacgcgc gcaactccgg cgaaagtttc agtccgtcaa taaatttctc
     7501 gcgcgcaggc gacccgtcac gct
//
LOCUS       DASTPM010000173         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_899771, whole genome shotgun sequence.
ACCESSION   DASTPM010000173 DASTPM010000000
VERSION     DASTPM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899771"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1834)
                     /locus_tag="VFR42_03035"
     CDS             complement(<1..1834)
                     /locus_tag="VFR42_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0368156.1"
                     /translation="MDVQISSKDRVGARYVFQQSLATGLLAGAGRFAAGAWVDIPARD
                     QQIALDWTHTFSGSFVNQVRYSFSRAGFGFEGGSFPNCTRAGINDCPTNISLPTSLVS
                     FGMQNNLPQGRTINNTQVQDNASITRGRHTFKVGGEFYKQRSPNVFLPNTNGSYNISG
                     SNAGGSCAAAFPLLSSTSSAPCGFSRFLADSPGSLSLTDGTPKFNFKEYDVAAYFGDD
                     WRVKDNLTINLGLRWEYSSQAINLLHDLTVANQAGSNPFWNTSLPASVTTLPTIPNHY
                     KYFGPNVGFAWNPHLLGMTGSKTVIRGGFRITYDPAYYNMFLNVATSAPVVNAGSISA
                     AACTAPCLPTSGFLGTDVRTAHLGDIPRGVNPGVRNYTQVASNFHEPYTQSWSLGVQH
                     EISSKIVFESRYVGNRVVGNFQTVNANPSLAGLIANGFSSFIPAGVTPCATSGTPGFA
                     SKRENCDFTNVRNRENTAFSTYHGLQNELRLRGFHGLSGGFSYTFSKTMDNASEIFST
                     FSGGNTVAGAQNPFDISKGEKALSGLDFPHTASIYFIYDLPFFKAQKGFLGHALGGFQ
                     TSATWRYSIGQLWTPTQLAGISTSCQTSFDTAFFNPASACRPFAG"
BASE COUNT          513 a          584 c          636 g          464 t
ORIGIN      
        1 tccctgcgaa tggacggcat gcagacgccg ggttaaagaa agcagtatcg aagctagtct
       61 ggcacgacgt gctaattcca gccagttgcg tgggggtcca aagctgccca atggaatagc
      121 gccacgtagc acttgtctgg aagccaccca acgcatgccc gaggaatcct ttctgggctt
      181 tgaagaacgg caagtcgtag atgaagtaga tggatgcggt gtgcgggaag tcgagaccgc
      241 ttaacgcttt ttcgcctttc gaaatgtcga agggattttg cgcaccggcc acggtgttac
      301 cgccgctgaa cgtcgaaaag atttcacttg cattgtccat tgtcttgctg aaggtgtagg
      361 agaaacctcc actcaggcca tggaaacctc gcagtcgcaa ttcgttctgc aagccgtggt
      421 aggtggagaa cgccgtattc tcccggtttc ggacgttggt gaaatcacaa ttttcacgct
      481 tactggcaaa accgggcgtg cccgaggtgg cgcagggcgt cacacccgct ggaataaacg
      541 aactgaatcc gttggcgatt aagcccgcca aagaaggatt cgcattcacg gtctggaagt
      601 taccgacaac gcgatttccc acgtaacgag actcaaagac tatcttgctg gaaatttcgt
      661 gttggacgcc tagcgaccag ctttgcgtat agggttcgtg gaagttcgat gccacctgtg
      721 tgtaattccg cacgcctggg ttcactccgc gaggaatgtc gcccaaatgt gccgtgcgta
      781 cgtcggtccc gaggaacccg gaagtcggga ggcaaggtgc agtgcacgcg gccgccgaga
      841 ttgagccggc gttgacaacc ggagctgaag tggccacgtt caagaacatg ttgtagtacg
      901 cggggtcata agtgatccgg aagccgccgc ggataaccgt tttcgaaccg gtcatgccga
      961 gaagatgagg attccaagcg aacccaacgt taggcccgaa gtatttgtag tgatttggaa
     1021 tcgtcggtaa cgtcgtcacg gatgctggaa gggaggtatt ccagaaggga ttggaacccg
     1081 cctgatttgc aactgtcagg tcatggagaa ggttgattgc ttggctcgaa tattcccagc
     1141 gcagaccaag gttgattgtc aggttgtcct taacccgcca atcgtcgcca aagtacgctg
     1201 cgacatcgta ttccttgaag ttaaatttgg gtgtcccgtc tgttaacgac agcgaaccag
     1261 gtgagtcagc caggaaccgc gaaaatccgc acggggcact gctggtgctg gacaggagcg
     1321 ggaacgctgc cgcacaactc cccccagcgt tggaaccgct gatgttatag gatccattgg
     1381 tattcggcaa aaacacgttt ggcgagcgct gcttgtagaa ttccccgccg accttgaagg
     1441 tgtgacggcc acgcgtgatc gaggcattgt cctgcacctg cgtattgttg attgtacggc
     1501 cctgtggcag gttgttttgc atcccaaagc taacaagact ggtgggcagg ctaatattcg
     1561 tcgggcaatc gttaatgccc gctcgagtgc aattcgggaa cgagcctccc tcaaatccga
     1621 agcccgcgcg cgaaaagctg taccttacct ggttgacgaa actgccgctg aatgtatggg
     1681 tccaatcgag cgcaatctgc tgatcacgag ccggaatgtc aacccaagcg cctgcagcaa
     1741 agcggccggc gccagctaga aggccagtcg ctaaactctg ttggaaaaca taccgagcgc
     1801 ccacgcgatc cttactgctg atctggacgt ccacgcggcc agtgaactcg cgatcattga
     1861 acaatccggg cgggttccgc tcgaccggcg cgaattcaat gggagccgcc gtgactccat
     1921 cggaaacgac cagcgttgtg ggagtgccag taacatgcgg gttacctagt ccaacagaat
     1981 aaggaccgat cgcattcagc gcagctaccg ccagattacc cgggaaagcc gcttgcagct
     2041 gtgttaggcc gttgggcgtc tgcgtcaagg ccgagccgga actctgcgga ctgccggctt
     2101 cacggattct gtcgaaataa ggggagaaaa agaaccacgc cttatcccgc ttgattggtc
     2161 ccccaaaagt tcctccaaag cggttttcaa cataccg
//
LOCUS       DASTPM010000174         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_784559, whole genome shotgun sequence.
ACCESSION   DASTPM010000174 DASTPM010000000
VERSION     DASTPM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784559"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..678)
                     /locus_tag="VFR42_03040"
     CDS             complement(91..678)
                     /locus_tag="VFR42_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEU0368157.1"
                     /translation="MPFINFPAREINCKLVYYGPGLGGKTANLQWIYDHTGQNQKGKM
                     VSLATETDRTLFFDFLPLDLGTVRGFKTRFHLYTVPGQVFYEASRKLILKGADGVIFV
                     ADSQEERLDANFETMENLQEHLKEHNLKFESIPYVLQLNKRDLPNAMAVPELTRQLQK
                     KGEPVLEAVAVTGSGVFETLREVAKLVLAELKKSG"
     gene            1032..1538
                     /locus_tag="VFR42_03045"
     CDS             1032..1538
                     /locus_tag="VFR42_03045"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368158.1"
                     /translation="MVDPRVQAQLKLYDEALALFHQQKFSRAKQELEKVLEGPSKELC
                     ERARMHIKIAEQRMKPSAEQNPRTAEEHYQRGVAMMNVGRWDDARESLDKARKAAPKA
                     DYIHYALAALDCLTGEADSAMKTLKVAIELRPENRYHARNDEDFAFLQEDPRFTELLY
                     PEKDGTAG"
     gene            1652..2695
                     /locus_tag="VFR42_03050"
     CDS             1652..2695
                     /locus_tag="VFR42_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU0368159.1"
                     /translation="MKKRSSNAKRHARGLNVVALGGGTGLSTLLRGLKEFVTRRRDET
                     ATHQRPVSDLTAIVTVTDDGGSSGRLRRENRVLPPGDIRNCMVALSQDEALLGRLFQY
                     RFHAGRGLVGHNFGNLFLAALTHVTGDFAEAVRVSSKVLAIKGRIFPSTVSNVSLVAT
                     MGDGAKVHGETRITASKKPIRKLTLLPRTVRPLPKALEAIQQADLILLGPGSLYTSIL
                     PNLLIPEIAHAIAKSKAPRVYISNLMTQPGETSGYALADHLRAIQRHAPRRVIDWVVA
                     NRQAVSPEVAKRYRAQGAEPVAVDIPELQKLGYRVVLDNLLEEHGVIRHNPTRLARLL
                     LEEFLARPRPSHS"
     gene            2786..3538
                     /locus_tag="VFR42_03055"
     CDS             2786..3538
                     /locus_tag="VFR42_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0368160.1"
                     /translation="MRLRIHRKRPEPWAIRREKLKTTWTMPLWALEWFWEWTAYVLSN
                     WSFLEVLEYLSSFGVLIAVVFYFAESGDRIKQKHYQAWQVINTAQGKGGNGGRIDALQ
                     ELNADGIALVGVDLSDAFLQGIHLQNAHLSRANLSAADLRDSDLVSADISVANLRSVN
                     CRQCTLRNADLQHADLTDADFAGADLAGADISGATLANIDLSNANLTNLKWRDKPNLK
                     NANLFGVKNSPEGFLAYALQHGAVQDAADSQE"
BASE COUNT          771 a         1156 c          941 g          740 t
ORIGIN      
        1 aaatctaaaa ttttcgggga agaatgcttt cagggtcgtc gagggatttc tgttttgttc
       61 ggacgtccgc taatcaggaa gttctaaccg ttatccgctc ttcttcaatt ccgccagcac
      121 cagcttggcc acttcccgca aagtttcaaa cactccgctg cccgtcaccg ccaccgcctc
      181 cagcaccggc tcgcccttct tctgcaattg cctggtcagc tccggcaccg ccatggcatt
      241 cggcaaatcg cgcttgttca actgcagaac gtaagggatg ctctcgaatt tcaggttgtg
      301 ctctttcaag tgctcctgaa gattctccat ggtctcaaaa ttcgcatcca gacgctcttc
      361 ctgcgaatca gccacaaaga tcaccccatc cgcgcccttc aagatcagtt tgcggctggc
      421 ctcgtaaaaa acctggccgg gaacggtata taaatggaag cgcgtcttga agccgcgcac
      481 agtccccagg tcgaggggca gaaagtcgaa gaacagggtc cggtcggtct ccgtcgccag
      541 cgaaaccatc tttccctttt gattttgccc ggtgtgatcg tagatccatt gcaggttggc
      601 ggtctttccg cccagacctg gaccgtagta caccagcttg cagttaattt cgcgcgcagg
      661 aaaattgatg aaaggcacgt gtcgttggtc cgaattcggt cagagactta tagcacacgc
      721 gccgagggca ggccagcata tcgccagcgg gtgtcgcgtg ttccgcatcg cttctatttt
      781 ccgtgaaatc gcgccgcggc aggaaagatt ttcatcaggt tacgcccggg aggtgacgtt
      841 atcgtcatcg cggccttttc cctctcgttg acgcgcgaaa cctgctctgc tacattcccc
      901 gcatggcaaa agcgcataag ccctctgcgg gctcatcgag ccgggccaaa gcatccagtt
      961 ccgcttcgtc taagcatatg aaaaaaaaga acttacctcc tcctcaccct ccccagcgtg
     1021 gcggcgtcga attggtagac cctcgcgtcc aggcccaact caagctctac gatgaagccc
     1081 tcgccctctt ccaccagcaa aagttttccc gcgccaagca ggaactcgag aaggttcttg
     1141 aggggcccag caaagaactc tgcgagcgcg cgcgcatgca catcaaaatt gcagagcagc
     1201 gcatgaagcc gagcgcggag caaaatcccc gcaccgccga agaacattac cagcgcggtg
     1261 tggccatgat gaacgtaggc cgctgggacg atgcccgcga aagcctcgac aaagcccgca
     1321 aggctgcgcc caaggctgac tacattcact acgcgctagc agctctggat tgcctgacgg
     1381 gtgaagccga ttccgcaatg aagactttga aggtggccat cgagcttcgt ccggaaaacc
     1441 gctatcacgc gcgcaatgac gaagactttg ctttcctgca ggaagacccc cgtttcaccg
     1501 aactgctcta tccggaaaaa gacggcaccg ccggctgatt cgaatctcga ggaatttctc
     1561 ggcataatag ggttcgctgg ttcctctcta cgcaatcccg ggtaatccac tttgggaatc
     1621 tgagcccgtt tgacgtgaag aaacattcgt cgtgaagaaa cgcagctcca atgcaaagcg
     1681 ccacgcgcgc gggctcaacg tcgtcgcact cggcgggggc accggactat ccactttatt
     1741 acgcgggctc aaggaatttg tcacccgccg tcgcgacgag accgcgaccc atcagcggcc
     1801 ggttagcgac ctcactgcca tcgtcaccgt aaccgacgat ggcggcagct cgggccgcct
     1861 gcgccgcgaa aaccgcgttc tccccccggg tgacatccgc aattgcatgg tcgcgctctc
     1921 gcaggatgag gccctgcttg gccgactgtt tcagtatcgc ttccatgccg gtcgcgggct
     1981 cgttgggcac aatttcggca atcttttcct ggccgcgctc acgcatgtca ccggcgattt
     2041 tgccgaagcg gtccgcgtta gcagcaaggt cctggccatc aagggccgga ttttcccttc
     2101 gactgtctcc aacgtctccc tcgtcgcaac gatgggagac ggtgcaaagg tccatggtga
     2161 aacccgaatt accgccagca aaaaacctat caggaagctg acgctgttgc cccgcaccgt
     2221 ccgcccgctg cccaaagcac tcgaggctat tcagcaggcc gacctgattc tgctggggcc
     2281 cggttcgctg tacaccagta ttcttcccaa cctgctcatt cccgagatcg cccatgccat
     2341 cgcaaaatcg aaagccccgc gcgtttacat ttcgaatctc atgacccagc ccggcgagac
     2401 ctcgggttat gcgctcgccg atcatcttcg cgccatccaa cggcacgcgc cgcgtcgcgt
     2461 aatagattgg gttgtcgcca accggcaggc cgtgtcgccg gaggtcgcca aacgctaccg
     2521 cgcacagggc gcagagccgg tggctgtcga tattcctgaa ttacagaaac tcggctatcg
     2581 cgtcgtgctc gacaatctcc tcgaagaaca cggcgtgatc cgccataacc ccacgcgcct
     2641 ggcgcgcctt ctgctggagg agttcctcgc aagaccgcgt ccctcgcata gctgagacgt
     2701 tctgccgcgc actagctgcc cagccccgct tttttgcctc aagctcagcg ccattggaat
     2761 agaatcctcg ctccagaaaa ataggatgcg actccgcata cacagaaaac gcccagagcc
     2821 ctgggccatt cgccgcgaaa agctgaagac cacctggacc atgcctctgt gggctcttga
     2881 gtggttctgg gaatggaccg cctatgtgct cagcaactgg tccttcctcg aggtcctcga
     2941 atatctcagc agcttcggcg tcctgatcgc ggtggtcttc tatttcgccg agtccggcga
     3001 ccgcatcaag cagaagcact atcaggcttg gcaagtgatc aacaccgccc agggaaaggg
     3061 cggcaatggt ggccgcatcg acgctcttca ggaactcaac gcagatggca ttgctctggt
     3121 cggcgtcgat ctatccgacg cattcttgca aggcattcat ctgcagaacg cccatttgtc
     3181 acgcgccaat ttatcggccg ccgacctccg tgatagtgat ctggtttcgg ccgatatttc
     3241 cgtcgccaat ttgcgctcag tgaattgccg ccaatgcacg ctgcgtaacg ccgacctgca
     3301 gcacgccgat ctcaccgacg cggacttcgc cggcgcggac ctcgccggag ccgatatttc
     3361 cggcgcgact ctcgccaaca tagacctgag caacgccaat ctcaccaact tgaagtggcg
     3421 cgacaagcca aatctcaaaa atgccaattt gttcggtgtg aagaactcgc cggaaggttt
     3481 tctcgcctac gcgttgcaac atggcgctgt ccaagacgca gccgactctc aggaatgatt
     3541 caaaacgccc accgcatcag cagcccgccg gtcgtgtagc cggctccgac cgcggcaaga
     3601 agcaccaa
//
LOCUS       DASTPM010000175         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_496161, whole genome shotgun sequence.
ACCESSION   DASTPM010000175 DASTPM010000000
VERSION     DASTPM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496161"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFR42_03060"
     CDS             <1..1067
                     /locus_tag="VFR42_03060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368161.1"
                     /translation="GIGQRARIMGTVWFFAAYLPISNIVQLNATVAEHWLYLPSVGFL
                     LFLGGCALDLPRRFGTGLTGVAAITILALSARSATRSNDWSSEETFYKRTLAAGGTSS
                     RVGVNLAQIYSNRGEYAVAEKMFRHILETNPDYPIARNNLAGVLVHEGKTAEAESLLA
                     RDVQHAKEARVQYPRTWIAAFNVAHMRSRANDDAGAIAMLERARADYPEVWELISLES
                     ELLRRTQGPLTALRLIENYTRQNWWHHAAWLAEGRLYAENNDVGQSTRALRYAALLDV
                     HDVQALNLLAAISVGQNQLEQACATQRRAIAREPNQPRQYLMLAEILDKMGRTSEAPT
                     ARDDATRLKAIVTSPRIAAN"
     gene            1113..2108
                     /gene="ilvC"
                     /locus_tag="VFR42_03065"
     CDS             1113..2108
                     /gene="ilvC"
                     /locus_tag="VFR42_03065"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU0368162.1"
                     /translation="MPVRIYRDKQIRLEPLRGKICGVIGFGSQGHAHALNLRDSGFEV
                     VVGLHRGSKSRPAARAHKLAVLETSEAVGQSDIVFLALPDTKMPKIFVKQIAPYLRRG
                     QTLLFAHGFTVVYRTVIPPHNVDVVMVAPKGLGQMVRREFKAGRGVPALLAVENDFSG
                     QARALAFAWAKAIGCARAGVIETTFREETETDLFGEQAVLCGGTTALIRAAFEILVRA
                     GYAPELAYFECLHELKFIVDMIHEAGIAGMRDLISDTAKWGDLTVGPKIVDKHVHKKM
                     AMALEQIRTGKFAREFIREMGTGGKRYRALLQKGRRHPLEKTGRRLRALMDWRKK"
BASE COUNT          491 a          612 c          642 g          445 t
ORIGIN      
        1 gcggaatagg ccagcgggcg cgtatcatgg gtacagtctg gttttttgcc gcttatcttc
       61 cgatctcgaa catcgtgcag cttaacgcga ccgtggcgga gcattggctt tacctgccaa
      121 gcgtcggctt cctgcttttc ctcggcggtt gcgcattgga cttgccgcgg aggttcggaa
      181 ccggtctaac tggtgttgcc gcgatcacaa tcctcgcctt aagcgctcgc agtgcgactc
      241 gcagcaatga ctggagcagc gaagaaacct tttacaagcg aactctggca gccggtggga
      301 caagttcgcg cgtcggtgtg aacctggcgc agatttactc gaaccgcggc gagtatgccg
      361 tggcagagaa aatgttccgc cacattctcg aaaccaaccc agactacccg atcgcgcgca
      421 ataatctcgc cggtgttctg gtgcacgagg gaaaaactgc cgaggcagag agcctgcttg
      481 cacgggatgt ccaacacgcc aaggaagcga gagttcagta tcctcgcacc tggattgccg
      541 cattcaacgt cgcgcatatg cgttcgcgag cgaacgacga tgccggagcc attgcgatgc
      601 ttgagcgggc acgcgcagac tatcccgagg tttgggagct gatcagtctt gaatcggaac
      661 tgctgcgccg aacacaaggt ccccttacgg cgcttcgtct aatcgaaaat tacacccgtc
      721 aaaactggtg gcaccacgcc gcctggctgg cagaaggcag gctatacgcg gagaacaatg
      781 acgttggtca atcgacccgt gctctccgct acgctgcttt actcgacgtg cacgacgtcc
      841 aggcgttgaa tttgctcgcc gcaatttcgg ttggccaaaa ccagctcgaa caagcatgcg
      901 ctacccaacg ccgcgccatc gcacgcgaac caaatcagcc ccggcaatat ctcatgctcg
      961 ccgagatcct ggataaaatg ggacgcacgt cggaggcacc gaccgcgcgc gatgacgcca
     1021 cacgattgaa ggcgattgtg acttcgccgc ggatagcggc caattaaatt gaatttggcg
     1081 tcgggggccg cttcagccac tcttgtccgg ccatgccggt gcgaatttat cgcgacaagc
     1141 agatcagact cgagccactg cgcggcaaaa tttgcggcgt gatcgggttt ggctcgcaag
     1201 gccatgccca cgccctaaat cttcgggaca gcgggttcga agtcgtcgtt ggattgcacc
     1261 gcggtagtaa gtcgcgcccg gcggcgcgag cgcacaaact tgcggttctg gaaacatcgg
     1321 aagcggttgg gcaaagcgac atcgtttttc tcgcgctgcc ggacacaaag atgccaaaga
     1381 ttttcgtcaa acaaatcgcg ccgtatttac ggcgtggaca aacgcttctc ttcgctcacg
     1441 ggttcaccgt ggtctaccgg actgtcatcc ctccgcacaa tgtggatgtg gtaatggtag
     1501 caccgaaagg attagggcaa atggtgcggc gcgagtttaa agctggccgc ggcgtgccgg
     1561 ccttgctcgc agtggaaaat gactttagcg ggcaggcgcg ggcgctggca tttgcctggg
     1621 ccaaagcaat cggatgcgcc cgggccggtg tcatcgaaac aacttttcgg gaagaaacgg
     1681 agaccgatct tttcggggag caggccgtgc tgtgcggcgg aacaaccgcg cttattcggg
     1741 ccgcgtttga aatcttggtc cgtgctggtt acgcgccgga actggcctac ttcgaatgcc
     1801 tgcacgaact aaaattcatt gtcgatatga tccacgaagc cggaatcgca gggatgcgcg
     1861 acctgatttc cgacaccgca aagtggggcg atctcaccgt tggaccaaaa attgtggata
     1921 agcacgtcca caaaaagatg gcgatggcac tggaacaaat ccgcaccgga aaattcgcgc
     1981 gcgaatttat tcgggagatg ggaactggag ggaaacgcta tcgggcgttg ctgcaaaagg
     2041 ggcgacgtca cccactggaa aaaaccggac gtcgattgcg tgccttgatg gattggcgaa
     2101 aaaaatgaat tttccccacg cgctttttca agcgcgttgt tcgcggcgga aaattctact
     2161 gccgcttact cgccagtgaa attcccgtac
//
LOCUS       DASTPM010000176         5055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1072625, whole genome shotgun sequence.
ACCESSION   DASTPM010000176 DASTPM010000000
VERSION     DASTPM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072625"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(39..249)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(326..1288)
                     /locus_tag="VFR42_03070"
     CDS             complement(326..1288)
                     /locus_tag="VFR42_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368163.1"
                     /translation="MTKVRLCFHDRCFDGTASAALFYRFYRERINPSAEFHFTGMTHR
                     AAQPWESDLFDGDENVIVDFKYSNSPKVTWWFDHHQSAFLTPADAQNFKRNPGPHMFY
                     EPDYKSCTLLIADTAREKFGFETTPLRDLIHWGDIIDGAQFPTPQSAVELKEPATQLA
                     LVIEAAPEDGVPAKIIRDLAYTPLSEMVKLPIVAKHLGPLLERHQRSIEILRERCEAR
                     DGVIFFDVSDLDLEGYNKFIPYLLHPECVYSISVSASALRAKVSLGTNPWNQATADQN
                     LASLAEKFGGGGHQRVAAISFDPKELPRALVVARGLVETLRNRN"
     gene            complement(1550..2014)
                     /locus_tag="VFR42_03075"
     CDS             complement(1550..2014)
                     /locus_tag="VFR42_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368164.1"
                     /translation="MAFCKACGQEIGTATFCPKCGASQEAVAPAGAGAPAVAAPSSEG
                     LQENVAALLAYVLGWLTGIIFLLIDKRPYVKFHAAQSIVVFGGLTILRIGIGIVGGIF
                     GGLSGLGAIFALGTVISLVGLVLWILLMVKAYQHQNFRVPIAAGIADSIAGK"
     gene            2158..3438
                     /locus_tag="VFR42_03080"
     CDS             2158..3438
                     /locus_tag="VFR42_03080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368165.1"
                     /translation="MNSPQPNRANDPSDGKPQTAPAFSLPRDVTPAERRTLIAGGLGW
                     LLDAMDVMLFSLIVGYLLREFSMDTRTAGFLNSLTLIASAIGGFLFGFLADRIGRVRA
                     LMLSILVYSLSSAACAFAHTIPQLAFFRFILGLGMGGEWTTAAALIAETWRAEHRGKA
                     LGLMQSAYAIGEAVAALVVAIVLPHYGWRAVFLVGVLPALAVFWIQAKVPEPELWKQR
                     ARLGSRPQLTSLLRGETLRNGMIATTMNAFAMFGYWGLFIWIPGYLSLPVEQGGRGLS
                     LTKTTTFFLVLCAGKWLGYALFGFFADAYGRKRPYFAYLVIAAMLVPIYGLVHTPFWL
                     LVLGPLVGFFGIGFFSGFAAIASEIFPGEIRATAMGLSYNVGRGLSAVAPFAVGALAI
                     HYGIGPAFMMQAAAYLVAALLSLLLPETRGQQLT"
     gene            complement(3476..4327)
                     /locus_tag="VFR42_03085"
     CDS             complement(3476..4327)
                     /locus_tag="VFR42_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEU0368166.1"
                     /translation="MILVEEKGRSESLLVIRQTDHAFLAAFFARAWGNERFSKPQPNG
                     SFCLAVAEHDNGWSDWELQPTVDPKTHLPYSFMSIPTETHVAIYHRGIERLVKVDHYA
                     GLLVTLHASELYDRGRATMPGYSAKYVKANENQIVSEFVQRLRLQQLRLKTDLRANSA
                     IKGHAEEPQLKANAARLEVMDRLSLHFCMNPQQDVMLEAVPVNDQGEEVDMELHAEGG
                     GLIAVAPYPFRRDPLAVSIMARRVPKKIYSGDQEFQRTLASAQYFPLKFTLRARRAGG
                     FSRGVGL"
     gene            4603..>5055
                     /locus_tag="VFR42_03090"
     CDS             4603..>5055
                     /locus_tag="VFR42_03090"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEU0368167.1"
                     /translation="MRKVVLFGSAVLLAITAAATIHAQQDKSKRPSPPAAAKCDLGGG
                     KSISVDYSSPRAKGRKVFGGIVPYGEVWRAGANEATTFVTTGDLMLGGHHVPAGSYTL
                     FTIPAQDKWTLIISKKTGEWGIPYPGMDSDLARVDMKVSATPSAVENFT"
BASE COUNT         1007 a         1592 c         1372 g         1084 t
ORIGIN      
        1 agaaggagag aaccgcaaaa tacggtgcgg gatcgctgct cctcgatagc gctccactcc
       61 gaatgagccc ggcaagggca cttcgtagtg acagtgaaca ggccttagac ccgttcccca
      121 acgccggcct gtttcagctg caggcggcga ttcggcggaa tgaacttctt gctccctttc
      181 gcgcaccctc cgaagctgct cggtacctcg ggtcacgcta ctgttgggct ccgcgcctct
      241 accaagaacc ttttcagatt aagtcatgcc tccgtggaaa gcaaccaggt tatgggaagg
      301 caagccagac tatgcggtta ttctgctaat ttcgattgcg caacgtctcg accagcccgc
      361 gagccactac gagcgcccgc ggcagttctt ttgggtcgaa agagatggcc gcaacccgct
      421 ggtgtccacc gccgccaaac ttctccgcta gcgacgctaa attctggtcc gcggtcgcct
      481 gattccacgg attcgtgccc agcgagacct tggcgcgcag cgccgacgcg ctcacactga
      541 tcgagtaaac gcactcgggg tgaagcagat acggaatgaa tttgttgtag ccctcgagat
      601 ccaggtcgct gacgtcaaaa aaaataacgc cgtcgcgggc ttcacagcgc tcccgcagaa
      661 tctcaatcga acgctggtgc cgttccaaca gcggccccag gtgcttcgcg acaatcggga
      721 gcttcaccat ttcgcttagc ggcgtatacg ctaagtcgcg aatgatcttg gcaggcaccc
      781 cgtcttccgg cgccgcttcg atcaccaacg ccagctgcgt cgctggctcc ttcaattcca
      841 cggccgactg cggtgtcgga aattgcgcgc cgtcgatgat gtcgccccaa tgaatcaggt
      901 cccgcaacgg agtggtctcg aacccaaact tctctcgtgc ggtgtccgca atcagcaggg
      961 tgcaggattt gtagtccggc tcgtaaaaca tatgtggccc aggattcctt ttgaagttct
     1021 gcgcatcagc gggagtcaaa aacgcactct ggtgatgatc gaaccaccag gtcacttttg
     1081 gcgaattcga gtacttgaaa tcgacgatta cgttctcgtc gccgtcaaac agatcgcttt
     1141 cccacggctg ggcggcccga tgggtcattc ctgtgaaatg aaattcggcg ctagggttaa
     1201 tccgctcgcg atagaagcgg taaaaaagtg cggcggaagc ggtgccatca aagcaacggt
     1261 catgaaagca cagtcgtact ttggtcatag acgagacgtt agagcctttc gagtcatacg
     1321 gtcactgcta ccaccaccag caaagctttt taagatgctg gcagcggcgc ggaaagtcaa
     1381 gtaccgacgt ttcattaccg acgtttcatc gacggcctcc agaacctttt caggggcagg
     1441 ttgggacggg acttgcaccc gccagccgcc gaacctgcac ggcgtacagc aaaaagggac
     1501 cgcgtttcgc ggtccctgtg ttatccgtca ttccagactt gaagcgaact tatttgcccg
     1561 caatactatc ggcgattcca gcagcaatgg gcacacggaa attctgatgc tggtaggcct
     1621 tcaccatcag caaaatccat agcaccaagc cgaccagact gatcactgtc cccagcgcaa
     1681 agatcgctcc cagaccggag agtccgccga aaatgccgcc tacgattccg ataccgatgc
     1741 gcaaaatcgt cagcccgcca aaaaccacaa tcgactgcgc cgcgtgaaac ttcacatacg
     1801 gccgcttatc aatcaaaaga aaaatgatgc ctgtcagcca tccgaggaca tacgcgagca
     1861 acgccgccac gttttcctgc agtccttcgc tggaaggtgc cgccaccgcc ggcgcacccg
     1921 ctccagccgg cgcgaccgct tcctggctcg ctccgcactt aggacaaaac gtagctgtgc
     1981 ctatttcttg accgcacgct ttacaaaatg ccatttgttc ctccgagggg gttccgatgc
     2041 gcaggagggt agcaaaggcc ttggagacag gcaatggaaa tcctcctttc cgtcggtggc
     2101 gaaactaacc tacgattcgc ctgcgcacgc ggccaacctc aggtagaatc cgcaccaatg
     2161 aacagccctc agccgaatcg ggccaacgac ccgtcggacg gcaagccaca aaccgcgcca
     2221 gccttttcac ttccacgcga tgtcacgccg gcggagcgcc gcaccctgat cgctggcggc
     2281 ctcggctggc tgcttgacgc catggacgtc atgctgttct cgctcatcgt cggctacttg
     2341 ctacgcgaat tctccatgga cacgcgcacg gccggcttcc taaactccct cactctcatc
     2401 gcatccgcaa tcggcggatt tctctttgga tttctagccg accgcatcgg ccgtgtgcgc
     2461 gcgctgatgc tttccattct ggtgtactcg ctctccagcg ccgcgtgcgc gttcgcgcac
     2521 accatcccgc agctcgcctt cttccgtttc attctcggcc tgggcatggg cggtgaatgg
     2581 acgacggccg ccgcattgat cgccgaaacc tggcgcgccg agcatcgcgg caaggcgctc
     2641 ggcctcatgc agtccgcgta cgcaatcggc gaagctgtcg ccgccctggt tgtcgccatc
     2701 gtgctcccgc attacggctg gcgcgcggtc ttcctggtcg gcgtccttcc cgcgctcgcg
     2761 gtgttttgga ttcaggcgaa agtccccgag cccgagcttt ggaaacagcg cgcccggcta
     2821 ggatcgcgtc cccagctcac gtccctgttg cgcggcgaaa cgctgcgcaa tggaatgatc
     2881 gccaccacca tgaacgcctt cgccatgttc ggttactggg gcctgtttat ttggattccc
     2941 ggctatcttt cccttcccgt cgaacagggc ggccgcggtc tgagcctcac caaaacaacc
     3001 acatttttcc tggtattgtg cgccggcaaa tggctgggtt acgcgctatt cggatttttt
     3061 gccgatgcct atggccgcaa acggccatat ttcgcttacc tggtgattgc cgccatgctc
     3121 gtgccaatct acggcctggt ccacacaccg ttttggctac tggtactggg accgttggtg
     3181 ggatttttcg gaatcggttt cttttctggc ttcgccgcaa tcgccagcga aattttcccg
     3241 ggcgaaatcc gcgccacggc catgggtctc agctacaacg tgggacgagg gctttccgcg
     3301 gtcgcgccat tcgccgttgg cgcgttggcc atccattacg ggattggacc tgccttcatg
     3361 atgcaagcgg ccgcctatct ggttgcggcg ctactctcac tattgctgcc cgaaacccgc
     3421 ggccaacagc ttacctgaca tacgaaagag cgccctggcc cagaaaacgc agactctata
     3481 ggccaacccc gcgcgagaac ccgccggctc gtcgggcccg caacgtaaat ttcaggggaa
     3541 agtactgggc ggacgcaagc gttctctgaa actcctgatc accggaatag attttctttg
     3601 gaacacgccg cgccataatg ctgactgcga gcggatcgcg tcggaacgga tacggcgcaa
     3661 cggcaatcag accgccgccc tccgcgtgga gctccatgtc aacctcctcg ccctgatcgt
     3721 tcaccggcac cgcctccagc atcacgtcct gttgcggatt catacagaag tgcagcgaaa
     3781 ggcggtccat cacttccagt cgcgccgcat tggctttcag ctgcggctct tctgcatgac
     3841 cctttatggc ggagttcgct cgcaagtcgg tcttcagtcg taactgctgc agcctgagtc
     3901 tctgaacaaa ctcactgact atctggttct cgttcgcctt gacgtatttg gcggagtacc
     3961 ccggcatagt cgcgcgcccg cgatcgtaga gttcggacgc atgcagggtc accaggaggc
     4021 ccgcgtagtg atccaccttg acgagtcttt cgatgccgcg gtgataaatc gcaacatgag
     4081 tttccgtcgg aatggacatg aagctgtatg gcaggtgggt ctttggatcc accgtcggtt
     4141 gcagttccca gtcgctccag ccgttatcgt gttcggcgac cgccaggcaa aacgacccat
     4201 tcggctgtgg cttgctgaat ctctcgtttc cccacgcgcg cgcgaagaaa gccgccaaaa
     4261 aagcgtgatc cgtctggcgg atgaccagca gcgactcgct ccggcccttt tcttcgacta
     4321 ggatcattcc gctgccccgc gttcgcagtt tcgccctgcg agaatcgctt cttgcttgtc
     4381 gcctttggtg attctatatt cctgcccggg tgcggggtgt ccgggctggg tcccaaggga
     4441 tttgtattat gcgctccacc ggcggtccaa tccacccgaa tcctctcatt taaccctgca
     4501 ggcgccaaac cgccgaggcc gcaacattcc agatcccgtg cctctttcct ttatgctttc
     4561 cgtgcgtcca tttgttagag tttctatccg ggaggctcca ccatgcggaa agttgttcta
     4621 ttcggaagtg cagtattgct ggccatcact gcggcagcta ccattcatgc gcaacaggat
     4681 aaatcgaagc ggccaagccc gccggccgcc gcgaaatgcg atctaggagg cggtaagtcc
     4741 atttcggtgg actattcgag tccacgggcc aagggacgta aggtctttgg cggcatcgta
     4801 ccctacggcg aagtctggcg cgccggcgca aatgaagcaa caacgtttgt gaccactggc
     4861 gatctgatgc tgggtggcca ccacgttcct gcgggcagct acaccctgtt taccatcccc
     4921 gcccaggaca aatggacttt gatcatcagc aagaagaccg gcgagtgggg cattccctat
     4981 ccgggcatgg attccgacct ggcccgcgtg gacatgaagg tttctgccac gccgagcgcc
     5041 gtggagaact ttacg
//
LOCUS       DASTPM010000177         3971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_438560, whole genome shotgun sequence.
ACCESSION   DASTPM010000177 DASTPM010000000
VERSION     DASTPM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438560"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2181
                     /locus_tag="VFR42_03095"
     CDS             <1..2181
                     /locus_tag="VFR42_03095"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HEU0368168.1"
                     /translation="DSVTVVDNGRGIPVDMHATEKRPAAEVVLTVLHAGGKFDSDTYK
                     VSGGLHGVGVSVVNALSDWLELEIQRDGAVWEQTYEKGKPTCKLKQTGKTRKTGTKVT
                     FHPDASIFEKTVFSYDTLATRLREYAFLNKGLKITLTDERTEKSAEFRFTGGIAEFVK
                     HLNRGKETLHDSPIYMDGKKGNVEIEIALQYNDTYGENVFAFANTINTVDGGTHLSGF
                     RSALTRTINNFASNAGMLKEQKDEVSISGDDVREGLVAVVSVRLPQPQFEGQTKGKLN
                     SDIKGDVEQLVNEKLGEWFDKHSTVARRIIGKCIDAARAREAARKARELTRRKSAMDS
                     SGLPGKLADCQERDPARCELFVVEGESAGGSAKMGRDRKYQAILPLKGKILNVEKARY
                     DKMLGHEEIRAIITALGTGIGKDDFDAGKLRYHKLILMTDADVDGSHIRTLLLTFFFR
                     HMKELIERGHVYIAQPPLYRVKRGKTEKYIRDERDFARELMKRATEDHTVKSKDGAAL
                     DGAKLTSFLLNVQEYEAAVAKLARRLREPELVELLAESGLEKKTDFEDKKALEKLLKE
                     VDKAKLKLDGKVIFDEEHSLYELQFGPPHNQKINWAVVSTAEFKRLRALSKQIDEFNH
                     PPFTVSMNGTKVTKDKAKDVLSYVVEDAKKDFTITRFKGLGEMNAEQLWDTTMNSDTR
                     TLLQVKLEDAVAAEEIFTTLMGENVEARRKFIEDNALEVVNLDI"
     gene            2240..2434
                     /locus_tag="VFR42_03100"
     CDS             2240..2434
                     /locus_tag="VFR42_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368169.1"
                     /translation="MSEFRAELTEHPDTQSGVAVQFASAAACDEFLKAAEAEFAVRRF
                     TNAADAFDTVKAYVFEHINK"
     gene            2492..>3971
                     /locus_tag="VFR42_03105"
     CDS             2492..>3971
                     /locus_tag="VFR42_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU0368170.1"
                     /translation="MADETNTSILLPIDIEAEMRKSYLDYAMSVIIARALPDVRDGLK
                     PVQRRILVAMNDLGLASNRGYRKCAKICGDTSGNYHPHGEAVIYPALVRQAQDFNMRY
                     PLVDGQGNFGSVDGDPPAAMRYTEARLAKITEDILADLEKETVDFIPNFDQTREEPVV
                     LPSRIPNLLVNGASGIAVGMATNIPPHNLREIVDAAILLIEKPDATLKEVLKIVPGPD
                     FPTGAYIAGREGIEAAYRTGRGSFIMRAKAAIEEAGKDRENIVITEIPYQVNKSRLIE
                     RIAELVQTKKIEGIGDVRDESSREGMRIVIELKRGEESQLILNNLFKHTQMQESFHMI
                     LLSIVAGQPRELGLIPMIKLFIEHRMDVVRRRTIFELRKAEEREHILIGYQIAVDHLD
                     NVIRIIRGSASRAEAKENLFKYFSEQDVTISEGGKSKKIEGVKLDGRKYRFAGAPEVE
                     AAGRGNALHLSYTQIDAILELQLHRLTQLSIDDILKELKSIRE"
BASE COUNT         1007 a         1167 c         1083 g          714 t
ORIGIN      
        1 gattccgtca ccgtcgtgga caacggccgc ggcattccgg tggatatgca cgccaccgaa
       61 aaacgacccg cggcagaagt ggtacttacc gttcttcacg ccgggggaaa attcgacagc
      121 gacacctaca aagtgtccgg cggtctgcac ggcgtcggcg tttccgtcgt caacgcgctc
      181 tcggattggc tcgaactcga aattcagcgc gacggcgccg tttgggagca aacctacgaa
      241 aagggcaagc ctacttgcaa gctcaagcag accggcaaaa cgcgcaaaac cggcaccaag
      301 gtcaccttcc accccgacgc ctcgatcttc gaaaagaccg tcttcagcta cgacaccctg
      361 gcaacgcgcc tgcgtgaata cgccttcctg aacaaaggct tgaagatcac gctgaccgac
      421 gaacgcaccg agaaatccgc ggagttccgg ttcaccggtg gaattgctga attcgtaaag
      481 catcttaatc gcggcaagga aaccctccat gattcgccaa tctacatgga cgggaaaaaa
      541 ggcaacgtcg aaatcgagat cgccctgcag tacaacgaca cctatggcga aaacgtgttt
      601 gccttcgcca acaccatcaa taccgtcgat ggcggcacgc atctctccgg ttttcgttcc
      661 gcgctcaccc gcactatcaa caacttcgcg tccaatgcag gcatgcttaa ggaacagaaa
      721 gacgaagtaa gcatctccgg cgacgacgtc cgcgaaggct tggtcgccgt cgtcagcgta
      781 cggctgcccc agccgcaatt tgaagggcaa accaaaggca agctcaacag cgatatcaag
      841 ggcgacgtcg agcaactcgt caacgaaaag ctgggcgaat ggtttgacaa gcactccacc
      901 gtggcgcggc gcatcatcgg aaagtgcatt gacgctgccc gcgcccgcga agccgcccgc
      961 aaggctcggg aactgactcg ccgcaaatcc gctatggatt cgagcggcct tcccggcaaa
     1021 cttgccgatt gtcaggagcg cgatccggcc cgctgcgaac tgtttgtggt cgagggcgaa
     1081 tcggccggcg gctcggcaaa aatgggacgc gaccggaagt atcaggccat cctcccgctc
     1141 aaaggcaaaa ttctcaatgt ggaaaaggcc cgctacgaca aaatgctcgg ccacgaggaa
     1201 attcgcgcca ttatcaccgc gcttgggacc ggcatcggca aggacgactt tgacgccggc
     1261 aagcttcgct atcacaaact gattctgatg accgacgccg acgtcgacgg ttcgcacatc
     1321 cgtactctgc tgctcacttt tttcttccgc cacatgaagg aactgatcga gcgcggccat
     1381 gtgtacatcg cccagccgcc gctctaccgc gtcaagcgcg gcaagaccga aaaatacatc
     1441 cgcgacgaac gtgattttgc gcgcgagttg atgaaacgcg ccaccgaaga ccacaccgtc
     1501 aaatcgaaag atggcgccgc gctggacggc gcgaaactca cttcgttcct gctgaatgtc
     1561 caggagtacg aggcagccgt ggccaagctc gctagacgct tgcgcgaacc ggaactggtg
     1621 gaactcctcg ccgagagcgg cctggaaaag aaaaccgact tcgaagacaa gaaagccctg
     1681 gaaaagttat tgaaggaagt ggataaggcc aaactgaagc tcgacggaaa agtgatcttt
     1741 gacgaagagc acagtctcta cgaactccag tttgggccgc cgcacaacca gaaaattaat
     1801 tgggcagtgg tttcgaccgc ggaattcaag cgtttgcggg cattgtcaaa acagatcgac
     1861 gaattcaacc acccgccgtt cacggtttcg atgaatggaa ccaaggtcac gaaagacaag
     1921 gcgaaggacg tcctcagcta cgtcgtcgaa gacgctaaga aagacttcac catcacgcga
     1981 ttcaaagggt tgggcgaaat gaacgccgag caactctggg atacgacgat gaactccgac
     2041 acgcggacct tgctgcaagt gaagctcgag gatgccgtgg cggccgagga aattttcacg
     2101 acactgatgg gcgaaaacgt cgaagcccgc cgcaagttca ttgaggacaa cgccctcgaa
     2161 gtcgtgaatc tggatattta gggcgagcgt ggtttaccaa ttgggttgga caacgttacc
     2221 tggattgcgt ggacttagcg tgagcgaatt tcgcgccgag ctgacggaac acccggacac
     2281 acaaagcgga gtggcggtgc aatttgccag cgctgcggcg tgtgatgagt ttctaaaagc
     2341 cgccgaagcc gagttcgcgg tacggcgatt cactaacgcg gcggacgcgt tcgataccgt
     2401 gaaggcttac gttttcgaac acatcaacaa atgatccacg aatttcgaat ttttgcaatt
     2461 cagaggcgta aataaccaag gctgaggacc aatggcggac gaaaccaaca catccatatt
     2521 gctgccgatc gatatcgaag cggaaatgcg caagtcgtat ctcgactatg ccatgagcgt
     2581 gattattgcg cgcgccctgc ccgacgtgcg cgatggccta aagccggtgc agcgccgcat
     2641 cctcgtggcc atgaacgacc tcggcctcgc ctccaaccgc ggctaccgca aatgcgcaaa
     2701 aatttgcgga gacacttccg gcaactacca cccgcacggc gaagcggtga tttaccccgc
     2761 actagtgcgt caggcgcagg acttcaacat gcgctatccc ctggtggatg gtcagggaaa
     2821 ctttggctca gtggacggcg atccccccgc ggccatgcgg tacaccgaag cccgcctggc
     2881 gaaaattacg gaagacattc tcgccgacct ggaaaaagag actgtcgatt tcattccgaa
     2941 cttcgatcag acacgcgaag aaccggttgt tctcccttcg cgcattccca atcttctggt
     3001 caacggtgcc agcggcatcg cggttggcat ggccaccaat attccacctc acaacttgcg
     3061 cgaaattgtc gacgccgcaa ttttgctgat tgaaaagccg gacgccacgc tgaaggaagt
     3121 cttgaagatt gttccggggc ctgattttcc caccggcgcc tatattgccg gccgcgaagg
     3181 catcgaagcg gcttaccgaa ccggacgcgg cagcttcatc atgcgcgcca aagccgccat
     3241 cgaagaggca ggcaaagacc gcgaaaacat cgtcatcacc gaaatccctt accaggtgaa
     3301 caagtcccgt ctgatagagc gcattgccga actggtgcag accaagaaaa tcgaaggcat
     3361 cggcgacgtt cgtgacgaat cttcgcgcga aggcatgcgc atcgtcatcg aactgaagcg
     3421 cggcgaagag tctcagctca tcctcaacaa tctcttcaag cacacccaga tgcaggaatc
     3481 gttccacatg attctgctct cgatcgtggc cgggcagccc cgcgaactgg gcctgatccc
     3541 gatgatcaag ctctttatcg agcaccgcat ggacgtcgtg cggcgccgca ccattttcga
     3601 attgcgcaag gcggaagagc gcgaacacat actgatcggc taccagattg ccgtcgacca
     3661 tctggacaac gtcatccgca tcatccgggg atccgccagc cgcgccgaag ccaaggaaaa
     3721 cctgttcaag tatttcagcg agcaggacgt caccatcagc gaaggtggaa agagcaagaa
     3781 gatcgaaggc gtgaagctcg acgggcgaaa gtaccgcttt gccggagcac ccgaagtcga
     3841 agccgctggg cggggcaatg cgctgcatct cagctatacg caaatcgatg ccatcctcga
     3901 actgcaattg catcgcctga cccagctttc tatcgacgac atcttgaaag agttgaagtc
     3961 gatccgcgaa c
//
LOCUS       DASTPM010000178         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_611815, whole genome shotgun sequence.
ACCESSION   DASTPM010000178 DASTPM010000000
VERSION     DASTPM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611815"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1895
                     /locus_tag="VFR42_03110"
     CDS             <1..1895
                     /locus_tag="VFR42_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368171.1"
                     /translation="RADLKRLRRDTDSGRISSLATSASAHAETVRSDASVPGSALNSA
                     VRESASSAGMAVRQAASGSRTRYVAAAALAVVLIAGAVYGAYHFGLGSRSASPPTKIT
                     QISHWDKAMFSPRLSPDGHTVAFVSPVDGISQVFVMLTSGGEPLQLTHDEGDKVARSF
                     STDGTEIYYRRSFGREEVWGVPTLGGVPRRVVTGDLLTPAPGGSFMYFVRRAERGIFR
                     ADKGGRGEELVYTWDQNTFPPGALLAYPDGKHLLMLTGDPTSFLERFRAYSIDVAARN
                     AEDLGEISGVAGGVAWSEPGKSIFLTRTVNGLTNIWKYNLQDKTFTQISFGPGPDYAP
                     MPDPAGHGLFIVNGKAADILSAYDVRTKQTTDIAGENATQPALSRDGKHLMYITIPSR
                     DRNALWVADVDGSNKRKLAEAPSLATGNWSPDSRFLAYADAEAGKTPKAYWVGADGSG
                     LHTLTWNGGEIQGVIWGGDQKSVFLNIFEKGKGPVIWKEAAEGGTPEKVAEGCGFTFD
                     VAPAGGYIFTLITQGERAGIYEFSLAEQKCLVLVPGVITFGLTVANDGKSFLYAVPSR
                     SDVTIYRQNWQDGKAVGQPQVALKLPFSFPLTTGGNAYDFTRDLSTVVYARPVGHAEL
                     YYLGDK"
     gene            2613..4010
                     /gene="lpdA"
                     /locus_tag="VFR42_03115"
     CDS             2613..4010
                     /gene="lpdA"
                     /locus_tag="VFR42_03115"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0368172.1"
                     /translation="MAKYDVAIIGSGPGGYVAAIRAGELGLKTVVVEKDPFLGGTCLH
                     VGCIPTKVLLHHADIYDHFKDGAELGFEVSGLKVNWASVISRKEKIVKKHAKGIEFLF
                     KKNKVEWVQGWGRYEGPGKVSIEKDGKQTTIEASNILMVSGSEARALPGIEPDHKQIL
                     TNRSILELPAIPKSLIVVGAGAVGVEFASIYNSFGTQVTILEALPRVVPVEDEEISVE
                     LDKAFKKKNIQIQTGCKVESVKKDAKGVTVSFSDKDGKKQTLQAEKLLLAVGRMPLTE
                     NCGLEKSKAKIERGFVHVGENMETAEKGLYAIGDIVAGLPQLAHAAMMEGIVAVTHIA
                     GKTTQQIRKDRIPNATYCEPQIGSIGLTEKQARDAGYAVKIGKFPFVGNSKATILGNH
                     GGFIKVVSEEKYGEVLGIHIIGPLATEILAEAAAVLNLEGTIDDLMNMVHAHPTVWEA
                     MGDAFASVRGLQINV"
     gene            4036..>4665
                     /gene="lipB"
                     /locus_tag="VFR42_03120"
     CDS             4036..>4665
                     /gene="lipB"
                     /locus_tag="VFR42_03120"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU0368173.1"
                     /translation="MNSCSVNDLGLIGYAEAWELQKRVVVARKAGLIEDVLLLCEHPH
                     VITLGRSGKRSNLLAGENVLRQKGVEYFETSRGGDITYHGPGQVVGYPILNLGAIKRD
                     VVWYVRTLEEVMIRTTADFGIAAQREAGKTGIWVGEDAAAQKLAAIGVHISRWVTSHG
                     FAYNVATDLRYFDLIVPCGIPGRKATSLEKLLSRCVSLNEVKSRLAVRFG"
BASE COUNT         1025 a         1237 c         1503 g          900 t
ORIGIN      
        1 tgcgggccga tttgaagaga ttgcggcggg atacggattc ggggcggatt tcctcgttgg
       61 caacgtctgc gtcagcccac gccgagaccg tccgttctga cgccagcgtg ccgggcagcg
      121 ccttgaattc cgccgttcgt gaatcagcaa gcagtgcggg tatggcagtg aggcaagcgg
      181 catctggcag ccgcaccaga tacgttgcgg cagcagccct ggccgtggtt ttgattgcgg
      241 gcgcagtgta tggtgcctat cactttggac taggctcccg cagtgccagc ccgccgacca
      301 agatcactca gatcagccac tgggacaaag caatgttttc gccgcggctg tcgcccgatg
      361 ggcacacagt ggcgttcgtt tcgccggtcg atggtatttc tcaggtgttc gtgatgctga
      421 cttcgggcgg cgaaccgctg cagctcaccc atgacgaagg cgacaaagtg gcgcgcagtt
      481 tttccactga cgggacagag atttactacc gcagaagttt tggcagagag gaagtttggg
      541 gcgttcccac attgggaggc gtcccgcggc gagtagttac tggagacttg ttgacgcccg
      601 cgcctggggg tagtttcatg tattttgtgc ggcgggccga gcgcggcatc tttcgcgcgg
      661 acaagggcgg ccggggcgaa gaactcgtct acacctggga tcaaaacacc tttccgccgg
      721 gggcgctgtt ggcctacccg gatggaaagc atctgcttat gctcacgggc gatccgactt
      781 cttttctcga gcgtttccgc gcctacagca ttgatgtagc ggcgcgaaac gcggaagacc
      841 tcggtgaaat atccggtgta gctggcggcg tcgcctggag cgaaccgggg aagagcattt
      901 ttctgactcg tacggtcaac ggactgacca acatctggaa gtacaatctg caggacaaaa
      961 cgttcacgca gatctcgttt ggacccggcc cggactatgc gccgatgccc gacccggcgg
     1021 gacatgggtt gttcatcgtg aacggcaagg cagcggatat tctctcggcc tacgatgtgc
     1081 ggacaaagca gacgacagat attgcgggag aaaatgccac gcagccggcg ctgtctcgcg
     1141 acggcaaaca cctcatgtac atcaccatcc cgtcgcgcga tcgcaacgca ctgtgggtcg
     1201 ccgatgtcga cggcagcaat aaaaggaaac tggcggaggc gccttcgcta gcgacgggaa
     1261 actggtcacc ggacagccgc ttcctcgctt acgcggacgc ggaagcgggg aagactccca
     1321 aggcgtactg ggttggcgca gatggcagcg gactgcacac actgacatgg aacggaggcg
     1381 agattcaggg tgtgatctgg ggaggggacc agaaaagcgt atttctgaac atattcgaaa
     1441 aaggcaaggg accggtgatc tggaaggagg cggcggaagg cggaacgcct gaaaaggtgg
     1501 cggaaggttg cgggtttacg tttgatgtgg cgcccgctgg cggttatatc tttacgttga
     1561 tcacgcaagg agagagggcc ggaatttacg aattttcgct ggccgagcag aagtgcctgg
     1621 tgctggtgcc aggagtgatt acctttgggc tgaccgtggc caacgacggc aagtcgtttt
     1681 tgtatgcggt gccttctcgc agcgacgtta cgatctaccg gcagaattgg caggacggca
     1741 aagcggttgg ccagccgcag gttgcgctga aactgccgtt ttcctttccg ctgaccacgg
     1801 gcggcaatgc ctatgacttc acgcgcgacc tctccactgt ggtgtacgcc cggccggtcg
     1861 gccacgccga gctctactac ctgggcgaca aataagcaca gattcgctgt agttcgcctc
     1921 ctgcgtcttt aaaaaacccg ggaaagacct gctcaaaaaa gtgagccgcc ggtgggggtg
     1981 ccggcggctc gggcgtgggt caggagggaa gttaaagaag gtcgattcgg tttgtcagcc
     2041 tcgcgatact gacctgcgct cgacgcggac ggttcccggt tcggggccgg ccacgctgag
     2101 tagcaatgtc accatcacgc taaaaaccag gccagccacc agcacggcgt gtctccgctg
     2161 ggaggttgtt ttccgggcct cccgcttaca aagccacttt gccgctcgac gccgcgcggg
     2221 tcactagacc ggcgggttta ttttgagggg taccggtgag ggaggacgct cgggtaccga
     2281 agtactgggc cccagcgcgg cgtcgaaagc gtcagaaggg gcgcggttgc ggggagtcac
     2341 tggcgctgct aaaattgcaa atttgccggg atgcagcagg ccgcaacagg gtagagggcc
     2401 gctgaaatcc cgtcaggaaa gaaacttcgc ggctgagggg gcccatcgtg aaaaacctgg
     2461 gccgatcttt atcgtgccag ccttgcgcga agtatgaaga ctcgccaaag aatgttcgcc
     2521 ggaaacagcc aggacaacgt ggattgccgt gaaaaaccac ggtctggggt gccgggcaat
     2581 tctgcaaggt tgccgaccga agggagattc gagtggccaa gtacgatgta gcgattatcg
     2641 ggagcggacc gggcggctat gttgccgcca ttcgcgccgg cgaactggga ttgaagacgg
     2701 tggtggtgga aaaggatcca ttcctgggcg gcacctgcct gcacgtcggc tgcattccca
     2761 ccaaagtgct gcttcatcat gcggatattt atgaccactt caaggatggc gcggagctgg
     2821 gatttgaggt cagcggcctg aaagtgaatt gggccagcgt gatttcccgc aaagagaaaa
     2881 tcgtcaagaa gcacgccaag ggcatcgagt ttcttttcaa gaagaacaaa gtcgagtggg
     2941 tgcagggctg gggccgctac gagggaccgg gcaaagtcag catcgaaaaa gacggcaagc
     3001 aaaccaccat cgaagccagc aacattttga tggtcagcgg cagcgaagcc cgtgcacttc
     3061 ctggcatcga gccggaccac aagcaaattt tgaccaaccg gtcgattctg gagctgccag
     3121 caattcccaa gagtctgatc gtggtaggcg ctggcgcagt cggcgttgag ttcgcctcta
     3181 tttacaattc gtttggcacc caggtgacga ttctggaggc tctgccgcgc gtcgttccgg
     3241 tggaagacga agaaatttcc gtggagctcg acaaggcatt caagaagaaa aatattcaga
     3301 ttcaaaccgg ctgcaaagtc gaaagcgtca agaaagacgc caagggcgtt accgtttcgt
     3361 tcagcgacaa agatggaaag aagcagacgc tgcaggcgga aaagctgctg ctggccgtgg
     3421 ggcgcatgcc gttgacggag aactgcggcc tggaaaaatc caaggccaaa attgaacgtg
     3481 gctttgtgca cgtcggcgaa aacatggaaa cggcggagaa ggggctatac gcgatcggcg
     3541 acatcgtcgc cggtctgccc caattggcgc acgcggccat gatggaagga atcgtcgccg
     3601 tcacccacat cgccggaaaa acaacgcagc agatccgcaa ggaccgcatt cccaacgcga
     3661 cgtattgcga accgcaaatt ggctccatcg gccttacgga aaagcaggct cgcgacgcgg
     3721 gctacgccgt aaaaatcggc aagttcccat ttgttggcaa cagcaaggcc accatcctcg
     3781 gcaatcacgg cggcttcatc aaagtcgtca gcgaagaaaa atacggtgaa gtgctgggca
     3841 ttcacatcat cgggccactg gccacggaaa ttctagccga ggcggcggcg gtgttgaacc
     3901 tggaaggcac catcgacgat ctgatgaaca tggtgcacgc gcatccaacg gtatgggaag
     3961 ccatgggcga cgcgtttgcc tcggtgcgcg gtttgcagat taacgtgtaa tggtaggatt
     4021 cgtgacgggt gactcgtgaa cagctgttcg gtcaacgatc ttggcttgat tggctacgcc
     4081 gaagcgtggg agctgcagaa acgcgtcgtc gtggcgcgca aggctggatt gattgaggat
     4141 gtgctgctgc tttgcgagca tccgcatgtg attacgctgg gccgcagcgg gaagcgctcg
     4201 aacttactgg ccggcgagaa cgtgctgcgc cagaagggcg tcgaatattt tgagacttcg
     4261 cgtggcggcg acatcaccta tcacggcccg gggcaggttg tcggttatcc gatcctgaat
     4321 ctcggcgcca tcaaacgcga tgtggtctgg tacgtacgca cgctggaaga agtgatgatt
     4381 cgcactaccg cagatttcgg aatcgcggca cagcgcgagg cgggcaagac aggtatttgg
     4441 gttggcgaag atgcagctgc gcaaaaactt gccgccatcg gcgtgcacat cagccgctgg
     4501 gtcacttcgc atggcttcgc ctacaacgtg gccaccgatt tgcgttactt tgatctgatc
     4561 gtgccttgcg gcatccccgg gcggaaagcg acatcgctcg aaaaattact gagccggtgc
     4621 gtgagcttga atgaagtgaa gtcgcgcctg gccgtgcgct tcggc
//
LOCUS       DASTPM010000179         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_784727, whole genome shotgun sequence.
ACCESSION   DASTPM010000179 DASTPM010000000
VERSION     DASTPM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784727"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFR42_03125"
     CDS             <1..427
                     /locus_tag="VFR42_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0368174.1"
                     /translation="DQVTADLNAAADYGLKLPASSGKLFVAGFCWGGGQTFRFATNRG
                     DLSAAFVFYGPPPAKDAMARIKAPVYGFYGGNDARINATIPETAEAMKAAGKTYDPVT
                     YDGAGHGFMRAGETPDDTNPANKTAREQAWARWKSHMSK"
     gene            518..2230
                     /locus_tag="VFR42_03130"
     CDS             518..2230
                     /locus_tag="VFR42_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368175.1"
                     /translation="MFFWIEGSLALLCLLLAILISNKRWLWLEKIEHAFCRLAEKRIA
                     SVILIGFLALAARLVLLPLMAVPHPNINDEFSFLLASDTFAHGRATNPPHPMWVHFEN
                     LHELQQPTYASMYPPAQGVFLAAGQRVTGHPFAGVCLVVALFCAATCWMLQGWASPAL
                     ALLGGLFAIVRFGMFSYWANSYWGGATAALGGALVLGALPRMRQLQNPKAALTLGVGL
                     AILANSRPYEGLLLAIPVAVRLLIWLLQKKDVELRQAALRVVLPLVLVLLLTAAAMGY
                     YFWRITGNPFLMPQVLERNTYAVAPYFLWESPRPVPAYDHPFLRDFYVGTELSFYESN
                     RSLTAIAALTIVKIFNTWLFYLGPLLTLPLAISFFANSRKLSWRRINADTRFLLAVVA
                     CSLFGLSFETFFFPHYAAPIACVLLALSMRALGNVRQLKERGGPAGLFLSRSVPVVCL
                     ILLALRCASAPLHFSVTSDWPPTWYNAIPAVPNRAKVLAQLKHTPGMHLVLVQYGKTS
                     RSRNQWVYNDADVDASRVVWAWDMGPARNRELLDYFKDRRVWSIDPDRETSDIAPYHA
                     VGSF"
     gene            complement(2391..2672)
                     /locus_tag="VFR42_03135"
     CDS             complement(2391..2672)
                     /locus_tag="VFR42_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368176.1"
                     /translation="MFCESGMQQDDGRKVDKLSDREGLHPERGVSKPSEPFAQAGSGE
                     ADQEKDNCEPGGESCGGSGAGFIQSKSGKRSDDDGAIRNGGEGEPFSSK"
     gene            complement(2742..2903)
                     /locus_tag="VFR42_03140"
     CDS             complement(2742..2903)
                     /locus_tag="VFR42_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368177.1"
                     /translation="MAQQQGQDDRQQHPAQRLAGMLGIIGGEDQKNHVNQENVNGRAE
                     ATAGQRAGG"
     gene            complement(2989..>3602)
                     /locus_tag="VFR42_03145"
     CDS             complement(2989..>3602)
                     /locus_tag="VFR42_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU0368178.1"
                     /translation="KWHGRSVETGIFKEPVAGRVALHTLNLDGDAQADLTVHGGEYKA
                     VYCYPVEHYAYWKKELGRELPKGMFGENFTTEGLLEKDAHLGDRLRVGTAEVVVTQPR
                     LPCYKLGVRFGADDMVKRFLASGRTGFYLAVTRDGEVEAGEEIVRISRDANAVPVTEI
                     TRLYVTKRFGPDDVDSLRRIMKIESVPEDWKGYFQERMEKAGV"
BASE COUNT          630 a         1252 c          874 g          846 t
ORIGIN      
        1 cgatcaggtc accgccgacc tcaacgccgc cgccgattac ggcctgaagc tccctgcatc
       61 cagtggaaag cttttcgtcg ccggcttctg ctggggcggt gggcaaacct tccgctttgc
      121 cacgaatcgc ggcgatcttt ccgcggcatt tgttttctac ggaccgccac ccgccaaaga
      181 cgccatggcc cgcatcaaag ctcctgtcta cggcttctac ggtggcaatg acgcccgcat
      241 caacgccacc atccccgaaa ccgccgaggc catgaaggcc gccggtaaaa cctacgaccc
      301 cgtcacctac gacggcgccg gtcacggttt catgcgcgcc ggcgaaactc ccgacgacac
      361 caaccccgcc aacaagaccg cccgcgaaca agcctgggcg cgctggaaat cccacatgtc
      421 caagtgacgt gatgcgccaa caagcttatg attccagcct gaaatagttc catgccaacg
      481 agcccgcgaa aacaacgcgg actgaacgga cgcattcttg ttcttttgga ttgaaggttc
      541 tctcgcgctg ttgtgtttgc tgttggcgat cttaatttcg aacaagcgct ggctttggct
      601 agaaaaaatt gaacacgcat tctgccgctt agccgaaaag cgaatcgcct cagtcattct
      661 gatcggattt ctggctctcg cagcgcgcct cgtcctcctg ccgctcatgg ccgtgccgca
      721 ccccaacatc aatgacgagt tcagcttcct gctcgcctca gacactttcg ctcacggccg
      781 ggccaccaat cctccccacc ccatgtgggt ccacttcgaa aatcttcacg aactgcagca
      841 gcccacctac gcgtcgatgt atccgcccgc tcaaggtgtt tttcttgccg cgggccagcg
      901 cgtcaccggt catcccttcg cgggagtttg tctcgtcgtc gcgctattct gcgcggcaac
      961 ttgctggatg ttgcaaggct gggcttctcc cgcccttgca cttttgggcg gattgttcgc
     1021 catcgtgcgc ttcggcatgt tcagctactg ggccaatagt tattggggag gcgctaccgc
     1081 tgcgctgggc ggggccttgg tcctcggcgc gctgccgcgc atgcgccaac tacaaaatcc
     1141 taaagccgct ctgaccttgg gcgttggttt ggcgattctg gccaacagcc gtccttatga
     1201 gggattgctc ctggcgatcc cggtcgccgt acggctgttg atctggctct tgcaaaaaaa
     1261 ggatgtggaa ctccgccagg ctgcgctgcg cgtcgtcctt ccgctggtcc tggtcttgct
     1321 gctcactgcc gcggctatgg gctactactt ctggcgaata accggaaatc ccttcctcat
     1381 gccgcaagtg ctggagcgca acacctacgc cgtcgcgcca tattttttgt gggaatctcc
     1441 gcgtcccgtg cccgcctatg atcatccgtt cttgcgcgat ttctacgttg gcactgagct
     1501 gtccttctat gagagcaacc gcagcctgac cgccatcgcc gccctgacca tcgtaaaaat
     1561 cttcaacacc tggctcttct acctgggacc gcttctcaca ctcccgttgg ccatttcttt
     1621 cttcgcaaat tcccgcaagc tctcctggcg cagaatcaac gccgacacgc ggtttctgct
     1681 ggcggtagtc gcttgctcgc tgttcggcct ttccttcgaa acattctttt tcccccacta
     1741 cgccgcccca atcgcttgcg tactcttggc tctgtcgatg cgcgcgctag gcaatgttcg
     1801 gcaactcaag gagcgaggcg gtcccgccgg actttttctc tcgcgtagcg ttcccgtcgt
     1861 ctgcctgatc ctgctggcgc tgcgctgcgc ctccgcgcct cttcattttt ccgtcacttc
     1921 cgattggccg cccacgtggt acaacgccat tcccgccgtt cccaatcgcg caaaagtttt
     1981 ggcgcaactg aagcacaccc ccggaatgca ccttgtcttg gtgcagtatg gaaaaacgtc
     2041 ccgcagccgc aaccagtggg tctacaacga cgctgacgta gatgcctcgc gagtggtctg
     2101 ggcctgggat atgggcccag cccgcaaccg cgaactcctc gactacttca aagaccgccg
     2161 cgtgtggtcc atcgatcccg accgcgaaac gtctgacatt gctccttacc acgccgtggg
     2221 ttcattttag cggccgacct tcaggtcggc tcgctgcgtt ttttttcaat tggtaagaga
     2281 tggatcttgg tccttactct ccgactagtt ccaacgctat caccccgaat aagctgcctg
     2341 tctccttgca tctgccttat acttgcccca acttgtcggc cgattcctct tcatttcgac
     2401 gaaaacggct cgccttcgcc gccattgcga attgcgccat catcgtcgct tcgtttgcca
     2461 ctcttggact gaatgaagcc ggcgcccgag ccgccacacg attcaccgcc aggttcgcaa
     2521 ttgtcttttt cctgatcggc ttcgccgctc ccggcctgcg caaatggctc gctcggtttg
     2581 ctgactccgc gctctggatg caggccttcg cgatcgctca gtttgtccac tttgcggccg
     2641 tcatcctgct gcataccgct ttcgcagaac acggtttgca tctccgtatc ggggaaggtg
     2701 cgatcgcgct cgccggtttc acggccgtcg cgggaatcgg actaaccgcc agccctttgc
     2761 ccggccgtcg cttccgctct gccattcaca ttttcctgat ttacgtgatt cttctgatcc
     2821 tcgccgccga ttattcccag catcccagca agccgctgcg ctggatgttg ttgccgatca
     2881 tcctggcctt gctgctgcgc catctctggc gccggacgcc aatccagaat ggcggcgaac
     2941 agatccagga aagttcccgc gaaccgcgcg cagcaaacct tctcgtatct aaacgccggc
     3001 cttttccatt cgttcctgga aatacccctt ccagtcctca gggaccgact cgatcttcat
     3061 gatgcgccgc agggaatcca catcgtccgg tccaaatcgc ttggtgacat acaggcgcgt
     3121 aatttccgtc accggcacgg cattcgcgtc tcgcgaaatt ctcacaattt cctctcccgc
     3181 ctcgacttct ccgtcacgcg tcaccgcgag ataaaatccc gtccgcccgc tggccagaaa
     3241 ccgcttcacc atgtcgtccg cgccgaaccg cacgcccagc ttgtagcacg gtaaccgcgg
     3301 ttgcgtcacc accacttccg cagtacccac gcgcaaccgg tcgcccaggt gggcatcctt
     3361 ttcgagcaat ccctccgtcg taaaattttc cccgaacatc cccttcggca attcccgccc
     3421 cagctctttc ttccaatacg cgtaatgctc caccggatag caatacaccg ctttatactc
     3481 gcccccgtgt accgtcaaat ccgcctgcgc gtctccatcc aggttcagcg tatgcagcgc
     3541 cactcgccct gctactggct ccttgaaaat ccctgtctcc acactccgcc catgccactt
     3601 ca
//
LOCUS       DASTPM010000180         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_179215, whole genome shotgun sequence.
ACCESSION   DASTPM010000180 DASTPM010000000
VERSION     DASTPM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179215"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFR42_03150"
     CDS             complement(<1..221)
                     /locus_tag="VFR42_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368179.1"
                     /translation="MASPVRIYAGTQEGLFVWRSKNGSWENVAVSFETGTIDSIDGLR
                     SQPNVVYLGVTQDGLYRTDDAGKSWCRVF"
     gene            complement(356..817)
                     /locus_tag="VFR42_03155"
     CDS             complement(356..817)
                     /locus_tag="VFR42_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0368180.1"
                     /translation="MKITISTTINGQQQETTVRPNQTLLEFLRDDLSLKGSVEGCGVG
                     VCGSCTVLVDGRPVSSCLMLATNAEGKMITTIEGLSQSGELDPVQQAFLKHQAFQCGY
                     CTPGMIMAVKGLLASNPHPTEEQARDYLSGNLCRCGTYVEVLAAVKELAER"
     gene            complement(814..>2332)
                     /locus_tag="VFR42_03160"
     CDS             complement(814..>2332)
                     /locus_tag="VFR42_03160"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0368181.1"
                     /translation="LLARKARRPVQWVLTRDEVFHTGRRYGAVVKIKTGVKRDGAIVA
                     RKVEVFYEIGAYALNGPVNAKTGCYVSSGPYNIPSRSLSTYSVYTNLPPTGPFRGVGV
                     SHVCWAYESEMDDIARRLGIDPLALRLKHLVKEGDVFVTGEKLVSVGITDCLQRTAAA
                     IGWKGKEEQSAPTAGMNVVCGKGLAVTIKSTTTPTTSSANVRLNADGSAILLTSSVDM
                     GQGALTSLAQIVGDELGLPFEKVSVSFPDTDITPYDKSSSSSRTTFHMGQAAQKAAGE
                     IRNQLLEVGAKKLEARIEDLELRDGAVWVRGVPDKRLSITEIFRARFGSSVGSMFGGY
                     CFKTEGGLNPKTGKGKAAAFWFFSACGVEVEVDTETGKVRVLRIVTAVDAGKAIHPKQ
                     CGLQNEGSMLSGMGSALFEEMIYDNGQPINSNFLDYMLPSMEDQPLEFCSILVETPHP
                     EGPSGAKGVGEAALPPVAPAIGNAIANALGGIRIRDLPVRPDKIVAMINAAKEKR"
BASE COUNT          469 a          724 c          627 g          512 t
ORIGIN      
        1 tcgaacaccc ggcaccagct cttgccggcg tcgtccgtgc gatagagtcc atcttgtgtg
       61 actccgaggt aaacgacgtt gggttggctt cttagcccgt cgatggaatc gatcgtgccg
      121 gtctcgaagg agaccgccac gttctcccag gagccattct tggaacgcca gacgaacaaa
      181 ccttcttggg tgcccgcata gatgcgaacg ggagaggcca tgagcaattc ctcctttgtc
      241 tgaaacgtcg atactggata cttgcttact ggagtgtgga gtactggagt attgggaaga
      301 caggaatccg agtcacccgt cactccaaca ctccattact ccatcactcc atttttcatc
      361 tttccgctaa ctctttgacc gccgccagca cctccacata ggtgccacaa cggcagaggt
      421 tccccgaaag ataatcgcgt gcctgttctt ctgtaggatg cggattactc gcgagcagtc
      481 ccttgaccgc catgatcatc cccggcgtgc agtagccgca ctggaacgcc tgatgtttga
      541 gaaaggcttg ctgcaccggg tcgagttcgc cgctctgcga caaaccttcg atggtagtaa
      601 tcatcttacc ctcggcgtta gtggcgagca tgagacagga gctcacgggc cggccatcga
      661 ccaggacggt gcaggaaccg cacacgccga cgccgcagcc ctcgaccgaa cctttgagcg
      721 agagatcgtc gcgcaagaac tcgagtagcg tctgattcgg tctaaccgta gtctcttgtt
      781 gttgcccgtt gatcgtggtc gagatggtga tcttcacctt ttttccttgg ccgcgttgat
      841 catcgcgacg atcttgtccg gcctgaccgg caagtctctt atgcgaatgc cgcccagcgc
      901 gttggcgatg gcgttgccga tggccggtgc caccggcggc aacgccgctt ctcccactcc
      961 cttggcgccg gagggaccct cggggtgagg cgtttcgacc aagatggaac aaaactccag
     1021 cggctgatct tccatggatg gaagcatgta gtccaagaaa ttgctattaa tcggctggcc
     1081 attgtcgtaa atcatttctt cgaaaagcgc cgagcccata ccggacaaca tcgacccttc
     1141 attctgcaac ccgcactgtt tgggatgaat cgctttgccc gcgtccaccg ctgtcacaat
     1201 gcgcaagacg cgcaccttgc ccgtctccgt atcgacctcg acttcaacac cgcaggcgga
     1261 gaagaaccag aatgccgcgg cctttccttt tcccgtcttc gggttcaaac ccccttcggt
     1321 cttgaaacaa taaccgccga acatgctgcc gactgacgat ccaaagcgtg ccctgaaaat
     1381 ttccgtgatc gataggcgtt tatcgggcac gccgcgcacc cacacggcgc catccctcaa
     1441 ttctagatcc tcgatccgcg cttcaagttt tttcgctccg acttccagca actgatttct
     1501 gatctcgccc gccgccttct gcgccgcctg ccccatgtga aaggtcgtgc ggcttgagct
     1561 cgacgactta tcgtagggcg taatgtcggt atccgggaag ctcaccgaga ctttctcaaa
     1621 aggcaatccc agctcgtcgc cgacgatttg cgccagcgaa gtcaacgccc cctgtcccat
     1681 gtcgacgctg ctggtcagaa gaatagccga gccgtcggcg ttaagccgga cgttagccga
     1741 cgaagtcgtc ggtgtggtcg tgcttttgat tgtaaccgca agccctttgc cgcagaccac
     1801 gttcattccg gcggttggcg ccgactgttc ttccttcccc ttccagccga tggccgcggc
     1861 cgtgcgctgc aagcaatcgg tgatgccaac ggaaaccagc ttttcgccag tgacgaagac
     1921 atcaccctct ttgacgaggt gtttcaagcg caatgcgaga ggatcgatcc caagccgccg
     1981 cgcgatgtcg tccatctcgg attcgtaagc ccaacacaca tgggagacgc cgacgccgcg
     2041 aaacgggccg gtgggcggca aattggtgta gacggagtaa gtcgacagac tccggctggg
     2101 aatattgtag ggaccgctcg atacataaca accggttttg gcgttgaccg gcccattgag
     2161 cgcgtaagcg ccgatttcat aaaagacctc gactttgcgc gcgacgatgg cgccgtcccg
     2221 cttcactccg gttttgattt ttaccaccgc gccgtagcgt ctgccggtgt gaaacacttc
     2281 gtcccgcgtc aaaacccact gtacgggccg ccgcgccttg cgcgcgagca gg
//
LOCUS       DASTPM010000181         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_121914, whole genome shotgun sequence.
ACCESSION   DASTPM010000181 DASTPM010000000
VERSION     DASTPM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121914"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..551
                     /locus_tag="VFR42_03165"
     CDS             111..551
                     /locus_tag="VFR42_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368182.1"
                     /translation="MADWKMLATVIGAAIAGIALIAYAFFRPTVDPEEAERKRRLHLN
                     QIGRIAEGQVVELAEHQAEPQPAPRGLFGAKAQPLVENRPRHLVSYSYAISGVTYHTA
                     QDITGLEGQVSMERLVAGQPASIKYDPSNPVDSILVADDWSGLR"
     gene            612..866
                     /locus_tag="VFR42_03170"
     CDS             612..866
                     /locus_tag="VFR42_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368183.1"
                     /translation="MKQIFASILTTVLIIALTLGAMFALVAATLKVTAIQSPLQRAFA
                     IGVELVLGIVLLLGTVWVATHLAVRIFHVQGPPSTGGPLA"
     gene            859..2448
                     /locus_tag="VFR42_03175"
     CDS             859..2448
                     /locus_tag="VFR42_03175"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU0368184.1"
                     /translation="MPEPKPSATPKSAQEKRLEELRRRSAEAEAGGGAERREREHQAG
                     KLTARERINLLLDEGTFEELDKFVRHHCTDFAMESQRPAGDGFVTGFGCIDGRLVYVF
                     AQDFTVFGGSLSESNAQKICKIMDLAMRNGAPVIGLNDSGGARIQEGVMSLAGYAEIF
                     LRNTLASGLIPQISAIMGPCAGGAVYSPAITDFIFMTRETSYMFVTGPDVIKTVTHEE
                     VSKQELGGALTHNEKSGVAHFISRDDADGCAMIRELMSFLPSNNLEDPPRRATNDSAT
                     RRDAALNTLVPEDAQKPYDIKDAIHSIVDDGYFFEVQEHFAKNLVVGFARMDGRPVGI
                     VANQPAYLAGVLDINASVKGARFVRFCDAFNIPLITFEDVPGFLPGTQQEFGGIIRHG
                     AKLLYAFAEATVPKITVITRKAYGGAYCVMASKHIRTDANFAWPTAEIAVMGPEGAVN
                     IVHKRELDKAPEAEREQLRQEKIAEFRERFANPFVAAERGYIDAVIEPAETRERIIVS
                     LRALENKRDNNPRKKHGNIPL"
     gene            2718..2960
                     /locus_tag="VFR42_03180"
     CDS             2718..2960
                     /locus_tag="VFR42_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368185.1"
                     /translation="MSKQLTVIAFAMFVLVGAMGLKSAVTSKDSGAVLMANGSAPTPP
                     TPKDWKRNGSAPTPPTPKDWKRNGSAPTPPTPKDWK"
     gene            3005..3724
                     /locus_tag="VFR42_03185"
     CDS             3005..3724
                     /locus_tag="VFR42_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368186.1"
                     /translation="MPGPAQYIVWLIGALLEATVVVCAFRRNAFRRYIFLNVYMASEF
                     VASAARFEMMRHYGFLSLAYAYFYYYSDALLTILLFLALSSLYSEVFRELNAERYVQM
                     GSVVLLAGTALFSFAVVHQSFTVAEQSSGKIFFHFVFEVSQNLYFVGLVLTYILWAAI
                     LKLRETRTRLIQLVLSLGVYFSLYAANYALRNLYPSAEAIFTVLNPLIGCFLPFAWAY
                     AFWKLPEEARLAPAELAVSPR"
     gene            3721..>4084
                     /locus_tag="VFR42_03190"
     CDS             3721..>4084
                     /locus_tag="VFR42_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368187.1"
                     /translation="MTAAFILVCSVGFLLMFFVSYCRSLLATSSTVALTQEVREVAGI
                     ANGISPDDFHRVVQLLQLCPDRPEERGRVRTVSAYYGFLGFVRSTLAVAVPSLKSWTD
                     RERAQCGYFAAVALGRRIA"
BASE COUNT          769 a         1160 c         1206 g          949 t
ORIGIN      
        1 cctcgggggc tgaggcgtaa tttgcgccgc tgcgaacgct gtgttcgcaa gaagtgctcc
       61 tcagtcttct cccgctgaac ttcttggggg ccgtaacgtt gaagaacttg ttggcagatt
      121 ggaagatgct ggccaccgtg attggagcgg ccatagccgg aattgcgttg atcgcgtacg
      181 cattttttcg tccgacagtg gatccggaag aggccgaacg caaacgccgg ttgcatttaa
      241 accagattgg ccgtattgcg gaaggccagg ttgtggagct ggcggagcat caagctgagc
      301 cgcagcccgc gccgcgcgga ttgtttggcg cgaaggcgca gccgctcgtg gaaaatcgtc
      361 cgcgccatct ggtttcttac agttacgcca tctccggcgt cacctaccac accgcgcagg
      421 acattaccgg tctcgaaggt caggtgagca tggagcgcct ggtggcgggc cagccggcca
      481 gcatcaagta tgatccttcg aacccggtgg attcgattct ggtcgccgac gactggtcgg
      541 gtttgcgcta agctaaagcg tcggcgggat ccgcgacact tatgggcctg tcgtgtttct
      601 gagaattttc catgaagcaa atcttcgctt ccattctgac gacggtctta atcattgcgc
      661 tcactcttgg cgccatgttc gcgttggtgg ccgcgacgct gaaagtgacc gctatccagt
      721 cgccactgca gcgagctttt gcgatcggtg tggagctggt tctcggcatc gtcctattgt
      781 tgggaaccgt ctgggtagcg acgcaccttg cggtacgcat tttccacgtg caggggccgc
      841 cgtctacagg agggccgctt gcctgagccg aagccgtctg ccacgccgaa gagtgcccag
      901 gaaaagcgcc tggaagaatt gcgccgccgc agcgccgagg ccgaggcggg tgggggcgcc
      961 gaacgccgcg aacgggaaca ccaagccggc aaactgaccg cgcgcgaacg gatcaaccta
     1021 ctgctggacg aaggcacgtt cgaagaactg gataagttcg ttcgccatca ttgcacggat
     1081 tttgcgatgg aatcgcagcg gccggcgggc gacgggtttg taacgggatt tggctgcatc
     1141 gatggccggc tggtctacgt gtttgcgcag gactttaccg tgtttggcgg gtcgctctcg
     1201 gaatcgaacg cccagaaaat ctgtaaaatc atggacctgg cgatgcgcaa tggcgcgccg
     1261 gtgatcggac tgaatgattc cggcggagcg cgcattcagg aaggtgtgat gtcgctcgcc
     1321 gggtacgcgg agatttttct gcgcaatacc ctggccagcg gcctgattcc gcaaatcagc
     1381 gccattatgg gcccgtgcgc gggcggagca gtgtattcac cggccatcac cgactttatt
     1441 tttatgacgc gcgaaacgtc gtacatgttc gtcaccggcc cggacgtgat caagactgtg
     1501 acgcacgaag aagtgagcaa gcaggagctg ggcggcgcgc tgacgcacaa cgagaaaagc
     1561 ggcgtcgcac actttatttc gcgcgacgat gccgacggct gcgccatgat ccgcgagctg
     1621 atgagctttc tgccctccaa caacctggag gatccgccgc gccgcgccac caatgattcc
     1681 gccactcgac gtgacgccgc gctcaatacg ctggttcccg aagacgccca aaaaccctac
     1741 gacattaagg acgcgattca ttccatcgtg gacgatggat actttttcga agtgcaggag
     1801 cacttcgcga agaatctggt ggtcggattc gcgcgaatgg atggccggcc ggttgggatc
     1861 gttgccaatc agcctgccta tctggcgggc gtgctggaca tcaatgcgtc ggttaaaggc
     1921 gcgcggtttg tacgtttctg cgacgcgttc aatattccgc tgatcacgtt tgaagacgtc
     1981 cccggtttcc ttcccgggac ccagcaggag tttggcggga tcattcgtca tggggcaaag
     2041 ctgctgtatg ccttcgccga agcgaccgtg cccaagatta cggtgattac gcggaaggcg
     2101 tatggcggag cctattgcgt tatggcctcc aagcacattc gaaccgacgc gaattttgcc
     2161 tggccaaccg cggaaatcgc ggtgatggga ccggaaggcg cggtcaatat tgtccataag
     2221 cgggagttgg acaaggctcc cgaggctgaa cgtgagcagc ttcgccagga aaagattgct
     2281 gaattccgtg aacggtttgc gaacccgttt gttgcggcgg agcggggcta catcgacgct
     2341 gtcattgagc ccgccgaaac ccgtgagcgc atcatcgttt ccttgcgcgc actggaaaac
     2401 aagcgagaca acaatccccg caaaaagcac ggaaacatcc cgctctgagg ggcgccttaa
     2461 cgggccaaat cgcgacaaaa acgcctgtcc gtaaagtcaa agttctagta ctactgtact
     2521 gttgtccaaa cgcctttctt ttctctatgc ttcccccgta agcagtgcct tccattcgct
     2581 ccggggatgt gcctcccctc ggcgattggc gcctgcttaa ggagaaattt ttttaggccc
     2641 gaaccttcgg gctgcttgat tattggtgtc gtctaagata ctgtaccccc gcggttgcac
     2701 catacctcgg aggcacaatg tccaagcagc tcacggtgat cgcatttgcg atgtttgttc
     2761 ttgttggtgc gatgggtctg aaatcagcag ttacttcgaa agacagcggt gcagtgttga
     2821 tggccaatgg cagcgcgcca acgccgccca ctcctaagga ttggaagcgg aatggcagcg
     2881 ctcctacgcc accgaccccc aaggattgga agcgcaatgg tagcgctcca acgccgccta
     2941 cgcccaagga ttggaagtag ctagcctgta gtcaaggcca gggcgtagac actaaggtga
     3001 atacgtgcct ggtccagcgc aatacatcgt ttggctcatt ggcgctctct tagaagcgac
     3061 cgtggtggtg tgcgcgttcc ggcgcaatgc gtttcggcgc tacatttttc tcaacgtgta
     3121 catggcgtcg gaatttgtcg ctagcgcggc tcgttttgag atgatgcgtc attacgggtt
     3181 cctttccctc gcctacgcgt acttctacta ctactcggat gcgctactga ctatccttct
     3241 gttcctggcg ctcagcagcc tctactcgga ggttttccgg gagttgaacg ctgagcgcta
     3301 cgttcaaatg ggttcggttg ttcttttggc gggtacagcc ctcttctcat tcgccgtcgt
     3361 ccaccaatcc ttcaccgtcg ccgagcaatc cagtggcaag attttctttc attttgtttt
     3421 tgaggtttcc cagaatcttt atttcgtcgg actggtgttg acctatattc tctgggccgc
     3481 cattcttaaa ctccgcgaaa cccgcactcg cctcatccag ttggtgctct ccctcggcgt
     3541 ttacttcagc ctttacgcgg ctaattacgc gcttcgtaat ctgtacccct cggcggaagc
     3601 catctttacc gtgctgaatc cgctgatcgg ttgcttcctg ccttttgctt gggcgtatgc
     3661 cttctggaag ctgccggaag aagcccgcct ggcgccggca gaactcgcgg tgagcccgcg
     3721 atgaccgccg ccttcattct tgtttgctcc gtcggctttt tgctgatgtt cttcgtgtct
     3781 tactgccgct cgttgctggc tacatcgtca accgtggctt tgacacagga agttcgcgaa
     3841 gtggcgggca tcgccaacgg tatctcgccg gacgattttc accgcgtggt gcagttgctg
     3901 cagctctgtc cggatcgccc tgaagagcgg ggccgcgtac gcacggtctc agcttattat
     3961 ggctttttgg gttttgtgcg gtccaccttg gcggtggcgg ttccttcgct gaagtcctgg
     4021 accgaccgcg aacgtgcgca gtgcggatac ttcgcggccg tggcccttgg ccggcgcatc
     4081 gcct
//
LOCUS       DASTPM010000182         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_6330, whole genome shotgun sequence.
ACCESSION   DASTPM010000182 DASTPM010000000
VERSION     DASTPM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6330"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFR42_03195"
     CDS             complement(<1..263)
                     /locus_tag="VFR42_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368188.1"
                     /translation="MGAAAGGHESTWSGGTGSSNGGFIAKKMGIADAQASTETINDAR
                     LAVRLVSNDGDVLWSTTQESKGAKYKGAGADAADKVVKQLLWD"
     gene            complement(572..2710)
                     /locus_tag="VFR42_03200"
     CDS             complement(572..2710)
                     /locus_tag="VFR42_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0368189.1"
                     /translation="MTDRPLSSIISFDSFELDLRTGELRKEGQRIKLQEQPFRVLSLL
                     VNRPGALVNREELHNSLWPADTFVDFDHGLNSAIARLREALRDSADKPRYIETVAKRG
                     YRFIAPLVEPARATLPPLRGLDAMPRNERPAETHTTKKVLVGVLLCLALVCAVGAWTT
                     FRGNPDAQLARIEVVPVGPLRGFQATPAISPDGTLVAFRQSGGSNDTGLYVAIVGGDK
                     PLQLTNGPGDCCPTWSPDGRQIAFTRYSEKSDAIYIVPALGGSEHRVYSSLASLDGGV
                     TWSADGRFLAFTESTAADLRSATVSVLSLADSSVRPITSPPPGWLDHEPAFSPDGTQL
                     AFVRSTVAGVSNDVFVMPATGGQARRLTFDNRPIMGAPTWTPDSHELVFSSNRGGVTG
                     LWRISAGGGVPRPLAGPVGAAMWPSIPAKAGALVYEQALSKLNIWRLNLKSAKQPERP
                     PSAIIAEKGEKMRPELSPDGKKIAFESNRLGFWDIWTCNTDSSDCQQVTSLHGTAGRA
                     RWSPDGRFLAFEFHPGERGEIYVVEVPGGTPRLMPTIPGADNLSPNWSRDGKWLYFAS
                     KRGNEPFQAWKIPAQGGSPVQLTKNGGISPVESPDGRFLYYSKYEQGGLWRLTLRGGE
                     ETEILSDLPGHAWPNWAVTPSGIYFLEFGKSRRGAIKFLDFAAHKILPLWTLENEPGW
                     GLSVSQDGKSIVYVQDEYAESSIMLVKNFR"
     gene            3033..3941
                     /locus_tag="VFR42_03205"
     CDS             3033..3941
                     /locus_tag="VFR42_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368190.1"
                     /translation="MGVGQEKVVGETVQAEKISREVEALRQRAAEGDSRAAYQLGRCY
                     MNGTGVPQDYREAATWYRRAAERDLPDAKFMMGYLYEQGKGVRRDYRQAVTYYTAAAK
                     QGHATAQNNLGSMYEHGQGVAKNIQEAAEWYRAAAKQGEVTAQCNVASLYFKGRGLPR
                     DNVQAAAWFRAAAERGYAPAQENLASMYYTGTGVALDYVEAANWVRRAAEGGYARAEL
                     DLGYLYEQGKGVPLDYVSAYMWYSSAAKKGERVGQEKLKELARLMTRQQISIAAARAA
                     ELPSAPGATISEDRSSATQSIFPKRP"
BASE COUNT          962 a         1150 c         1330 g          872 t
ORIGIN      
        1 aaatcccaaa gaagttgttt tacaaccttg tccgccgcgt cagcacctgc gcctttgtac
       61 ttcgcgccct tactctcttg tgttgtagac cacaatacat cgccatcatt cgaaacgagt
      121 cgaacggcaa gtcgggcatc attgattgtt tcggtagaag cctgggcatc agcaatgccc
      181 atcttcttgg cgatgaatcc tccattgctt gatcccgtac cgccggacca tgtgctttca
      241 tgcccaccag cagcggcgcc caccgacgtg gcatcagagc tggagtgcag aaactatttt
      301 ggaaatggtt ccggatcgcc aaggcggctg ccttccgccg tctcgctgcg cagccgatca
      361 agttcccgct gggcaaggag ttcttttcct tccaagttca agaattcaat ccgttcgcgg
      421 cggctcttga gcgcgctaga cggaccggtt gaagaaacca cgtgctcgtg accttgggaa
      481 gctagcgatt cggcgaaaat ccggagatgg ccgcccgcga ggatgatgca ggcgattcag
      541 acagcgatga aagctgcgag tggttttaat atcaacggaa attcttgacc agcatgatgc
      601 tggactcagc gtattcgtcc tggacgtaaa cgatggattt gccgtcttgg gagacgctca
      661 gaccccagcc gggttcattc tccagtgtcc aaaggggaag aattttgtgg gcagcaaagt
      721 ccaagaactt gatggcgccg cgacgcgatt ttccaaattc gagaaagtag atgccgctgg
      781 gtgtcaccgc ccaattgggc catgcgtggc ccggaaggtc gcttaggatt tcagtttctt
      841 cgccaccccg tagagtcaat cgccagagtc caccctgctc atacttggag taatacaaaa
      901 agcgtccgtc gggcgactcc accggagaga ttccgccgtt cttggtgagt tggacgggag
      961 agccgccctg agcgggaatc ttccaggctt gaaaaggttc attgccacgc tttgaggcga
     1021 agtacaacca cttaccatcg cgtgaccaat tcggggatag attgtcggca ccaggaattg
     1081 tgggcataag gcggggggtg ccgccaggca cctcgacaac gtagatttcg cctcgctcgc
     1141 cggggtgaaa ttcgaacgcg aggaagcgcc catcgggaga ccagcgggcg cgtccggcag
     1201 tgccatgcaa ggaagtgacc tgctgacaat cgctcgagtc ggtattgcat gtccaaatgt
     1261 cccagaagcc taagcgattc gactcaaatg caatcttctt cccatcgggc gacaattccg
     1321 gacgcatctt ctcgcctttc tcggcgatga tcgctgatgg gggccgttcc ggttgttttg
     1381 cagacttgag gttgagacgc cagatattga gttttgagag cgcctgctcg tagaccagcg
     1441 cacctgcttt tgcgggaata gacggccaca tggccgcgcc gaccgggcca gccagcggcc
     1501 tggggacgcc gccccccgcg gagattcgcc acagaccagt tacaccacct cgattcgagg
     1561 aaaacacgag ttcgtgacta tcaggagtcc aggtgggtgc gcccataatg ggccgattgt
     1621 cgaaggtgag gcgcctggcc tggccacccg tggcgggcat gacaaacaca tcgttactga
     1681 cacctgcgac cgtggaccgt acaaaagcga gctgtgtgcc atcgggcgaa aacgcgggct
     1741 catgatcaag ccaaccagga ggaggagatg taattggccg aacgctcgaa tctgctagcg
     1801 agagcaccga gacagtggcg ctcctgaggt ccgcagcggt gctctcggtg aaggcgagga
     1861 accggccatc ggctgaccag gtaactcccc catccagaga cgccaagctc gaataaacac
     1921 gatgctcgct gccgccgagg gcagggacga tatagatcgc gtcggacttt tcagaatacc
     1981 gagtgaaagc gatctgtcgg ccatcgggcg accacgtggg acagcagtcg ccggggccat
     2041 tggttagttg tagaggtttg tcacctccaa cgatcgctac gtagagacct gtatcgttcg
     2101 acccaccgct ttgccgaaat gccaccagtg tcccgtctgg cgaaatcgcg ggcgtagcct
     2161 ggaatccgcg caagggtccc acgggtacca cttcgattcg cgccaattgg gcatcgggat
     2221 ttccacggaa cgtggtccaa gcaccgacag cacacactaa ggccagacag aggagcaccc
     2281 caactagaac ctttttggtg gtgtgagttt cggcgggccg ctcgtttcga ggcatagcgt
     2341 ccagaccgcg aagcggcggt agcgttgccc gtgccggttc gaccaaaggc gcgatgaaac
     2401 ggtaaccccg ttttgccact gtttcaatgt agcggggctt gtccgcagaa tcgcgcaacg
     2461 cttcacgcag cctggcgata gcgctgttca gtccatgatc aaagtcaaca aaggtatccg
     2521 ccggccaaag gctgttatgg agttcttcgc gatttaccaa agcgcccgga cggttgacca
     2581 ataacgaaag aacccgaaag ggttgttctt gcaacttgat gcgctgccct tccttgcgca
     2641 attccccggt tcggaggtcg agctcgaagc tatcgaagga aatgatggaa gagagaggcc
     2701 gatcagtcat tgtgtcacct gtggattttg gacgtgaatt gtattgcagg cgttagtacg
     2761 gtgcatcggg ccgggtgtgg tttgcgaggt ctttggcaag tccaattaat tcaacagagt
     2821 atgcgattat ccgcgcgtgt gaaagatgtg ttcgcggttg cattgccggc cgcacggacc
     2881 gggcggagag ttgagcgcca gaggccggaa agaaagcgcg ttcgagtggc tttttgatct
     2941 ggcgttcacg gatgggggag acggccatgt tgagctcgcg aagactcgct gtaattacgt
     3001 tattcgggtc gatttggttc ggtaattcta gcgtgggggt cgggcaagag aaggtggttg
     3061 gcgaaaccgt ccaagcggaa aagatttcaa gggaggtaga ggccctccgg cagcgtgctg
     3121 cggagggaga ttcgcgagca gcataccagc tgggccggtg ttacatgaat ggcaccggcg
     3181 ttcctcagga ctaccgggag gcggccacgt ggtatcgacg ggccgcagag cgggatttgc
     3241 cggatgcgaa gttcatgatg ggctatttgt acgaacaagg caaaggtgtg aggcgagact
     3301 acaggcaggc agtgacttat tacactgcgg ccgccaaaca gggccacgcg acggctcaga
     3361 acaatcttgg ttccatgtac gagcacgggc aaggtgtggc aaaaaatatt caggaggcgg
     3421 cagagtggta tcgagcagcg gcgaagcaag gagaagttac ggcgcaatgc aatgtggcgt
     3481 cgctgtattt caagggccga ggcctgccgc gggacaatgt acaagcagcg gcatggtttc
     3541 gggcagcggc ggagcgcggg tatgcaccgg cgcaagagaa tcttgcttcg atgtactaca
     3601 ccggaacggg cgtggctctc gactacgtgg aggccgccaa ttgggtgcga cgggccgccg
     3661 aggggggata cgctcgcgcc gagctggacc tcggatatct ttacgagcag ggcaagggag
     3721 ttccgctgga ttacgtctcg gcttacatgt ggtacagctc ggcggcgaaa aaaggggaga
     3781 gggtgggcca ggaaaaactg aaagaacttg cgcggttaat gacgcggcaa caaatcagca
     3841 tagcggccgc ccgagcggcg gaactacctt cggcgccagg agcaaccatc tccgaggatc
     3901 ggtcaagcgc gacgcagagc atatttccca agcgaccctg aaggcggttt ccctgcggcc
     3961 agcacgtgtt cgctttctgg tagtgcctta caaatttcta ataaacgctc ttggatacca
     4021 cgctcgtcag aatagcaggc ggaaggatta gctgaatgtt atcctggaag tcagccacag
     4081 attgagccga tgtacctgca gtgtacctcg gttatcagtt cgggcagctt cagtcattct
     4141 cgcagggtgt cgggcaacct tctgaggatc gagcattgcg cggcccatga cgcgaacggt
     4201 ccgcggcggg ccaactggga aaaacgcccc cgcgttaact agcgggtttt gcctagtcct
     4261 ggaaaaaaga gcagcacgat tcgtcggagt gctacccagc cgacctttaa tccc
//
LOCUS       DASTPM010000183         3699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_121963, whole genome shotgun sequence.
ACCESSION   DASTPM010000183 DASTPM010000000
VERSION     DASTPM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121963"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VFR42_03210"
     CDS             complement(<1..490)
                     /locus_tag="VFR42_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368191.1"
                     /translation="MSASPSIAASPIKFGTSGWRGLIADDFTFANVRLAVTAIAEHAK
                     AKTAKPTILVGYDTRFYSEEFSALAVTILRQHGIRALLCETFAPTPAIAYEIQRAKLD
                     GAVNFTASHNPAQYHGLKFSSSDAGPALPEITKDLEARSAKLFAAGGVAPVKSDTSGA
                     ENV"
     gene            549..878
                     /gene="cutA"
                     /locus_tag="VFR42_03215"
     CDS             549..878
                     /gene="cutA"
                     /locus_tag="VFR42_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEU0368192.1"
                     /translation="MPAGMNAKVVLVTCGSRAEARRIAQAAVAKRLAACVNLVTVPVE
                     SLYRWKEKVERATEFLLVIKTTGARLKELEEEVARLHGYDVPEFLVIGVAGGSRKYLK
                     WLSQSVS"
     gene            complement(887..2047)
                     /locus_tag="VFR42_03220"
     CDS             complement(887..2047)
                     /locus_tag="VFR42_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEU0368193.1"
                     /translation="MFRLRTVNSVAQVLAALALLAALSGSSPTLFVAEQSRVTPPLYT
                     SGYQILDAKNHAVRLTSVNWYGFDQKEFVPGGLDHAPLAKIIEQIHALGVNSVRLPWA
                     NETLEKDPVVPDYAVTANPQFRGKHTMQIIDAVIAALAKARIMVILDNHVSRADWCCK
                     DDDGNGLWYNDDYPEEKWLADWRTMVLRYKGQPWVVGADLRNELRSGAAWGGSDPKLD
                     WHAAAERGGNTVLDANPNLLVMVEGPQYSTDFTAFAALPLTLKVPNRLVYSPHAYASA
                     SHTFASYDEMKHAYDTRAGFLLHHKPEVPLWVGEFGTCQTLDCGANSQWFAWFVQYLK
                     ENNLSWGYWPLNGTQSTGYSRTYDTLETYGLLSTDYQHIAAPKIAALFRTIQ"
     gene            complement(2040..3179)
                     /locus_tag="VFR42_03225"
     CDS             complement(2040..3179)
                     /locus_tag="VFR42_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU0368194.1"
                     /translation="MGFATNAIHVGQEPDPATGSIVAPIYQTSTYVQEELGKNKGYDY
                     ARTNHPNRKALEKTLAKLEGGHAAYAFSSGMAAIDSVFRLLRPGDHVVLSEAVYGGVF
                     RLTTQLLVHFGLEFSFVDTSSSDSVLAAMRANTRMLYVETPTNPTMRISDIAAMAKLA
                     SERNATLVVDNTFLSPYLQRPIELGAHIVVHSMTKYLNGHSDSTGGCVVLTRPEDTEK
                     IYFIQRSAGAGLAPMDCFLVSRGLKTLAVRMLQHNANGIAVARHLDAHAKVRKVYYPG
                     LASHPQHDIARKQQRGPGAMLSFDLGSLEAARRFLNQVRLCALAESLGGVESLISLPA
                     LMTHASMPKEVQERIGISEGLVRLSVGIEDVEDIIADLDQALLYV"
     gene            3486..>3699
                     /locus_tag="VFR42_03230"
     CDS             3486..>3699
                     /locus_tag="VFR42_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368195.1"
                     /translation="MKSTRFPVAALALLFFLALPLCAQDTPAKDSQSPDSADAATAVK
                     RLRIEVTGGERNVAVENASVYLKYVEE"
BASE COUNT          751 a         1032 c         1150 g          766 t
ORIGIN      
        1 caacgttctc ggcgccggag gtgtcgcttt tcactggagc gacgccgccc gccgcaaaga
       61 gttttgccga tcgcgcttcc aggtccttgg tgatttctgg cagcgccggt ccggcatccg
      121 acgaagagaa tttcagaccg tgatactgcg ccggattgtg gctggctgtg aagttgaccg
      181 cgccgtccag cttggcgcgt tgaatttcgt acgcaatcgc gggagtcggc gcaaaggtct
      241 cacacagcaa agcgcgaatc ccgtgctggc gcagaatggt cacggccagc gcggaaaatt
      301 cctcggagta aaatcgtgtg tcgtaaccga ccaaaatggt gggcttggca gtttttgctt
      361 ttgcgtgttc ggcgatggca gtcaccgcca gccgtacgtt ggcaaacgta aaatcgtcgg
      421 cgatgagtcc tcgccatccg gaagtgccaa acttgatcgg cgaagccgcg atacttggag
      481 aagcgctcat aatttggtta gtgtagccct tccggcatag cctgcaaata gcgcaaagag
      541 gagaaaccat gccagcggga atgaatgcga aagtggttct ggtcacatgc ggcagccggg
      601 ccgaagcgag gaggattgca caagcggcgg tggcgaaacg gctggcggct tgcgtcaacc
      661 tggtcaccgt cccggtcgaa tccttatacc gctggaaaga aaaggtggag agggcgacgg
      721 agtttctgct ggtgatcaaa accacgggcg ccaggcttaa ggaattggaa gaggaagtgg
      781 ctcggctgca tggctatgat gtgccggaat tcctggtgat cggagtggcc ggcggttcgc
      841 ggaagtatct gaaatggcta agccaaagcg tttcgtagcc gcgagattat tggatggtgc
      901 ggaagagtgc ggcgatcttc ggtgcggcga tgtgctggta atcggtcgaa agaaggccgt
      961 aagtttcgag tgtgtcgtaa gtgcggctgt agccggtcga ttgcgtgccg ttgaggggcc
     1021 aatagcccca gcttaaattg ttttccttca ggtattgcac gaaccaagcg aaccactggc
     1081 tgttggctcc gcagtcgagg gtctggcagg taccaaattc gccgacccac aatggaactt
     1141 caggcttgtg atgcagcaga aagccggcac gcgtgtcata ggcatgcttc atttcgtcgt
     1201 agctggcgaa ggtatgagaa gcggacgcgt aggcgtgcgg cgaatagacc agccgattgg
     1261 gaacttttag cgtaagcggc agtgcagcga acgctgtaaa atcggtggag tattgcggcc
     1321 cttcgaccat taccaataga ttcgggttgg cgtcgaggac cgtgttccca ccgcgttcgg
     1381 ctgcggcgtg ccaatcgagc ttgggatcac tgccgcccca ggccgctcca ctgcgcagct
     1441 cgttgcgcag atcggcgccc accacccagg gttgcccttt gtagcgcagg accatggtgc
     1501 gccagtcggc cagccacttt tcttccggat agtcgtcgtt gtaccagagc ccgttgccgt
     1561 cgtcgtcctt gcagcaccaa tcggctcggc tgacgtgatt gtcgagaatc accatgatgc
     1621 gcgctttagc gagagcggcg atgaccgcgt cgataatttg catagtgtgt ttgccgcgaa
     1681 attgcggatt agctgtgacg gcataatccg gaacgacggg atctttctcg agcgtctcat
     1741 tggcccaggg aagccggacg gagttgacgc cgagcgcgtg aatttgttcg atgatcttgg
     1801 cgagcggcgc atggtccagc ccgcccggaa cgaactcctt ctgatcgaag ccgtaccagt
     1861 tgaccgaggt aagccggacg gcgtggttct tcgcgtccag aatctggtag ccgctggtgt
     1921 agagtggcgg cgtgacgcgc gattgctcgg cgacaaacaa cgttggggag gagccggaca
     1981 acgcagccag aagagccaac gcggcaagaa cttgcgcaac ggaattcacg gttcgaagcc
     2041 taaacataaa ggagcgcctg gtccaagtcg gcgatgatgt cttctacgtc ttcgatgccg
     2101 accgacaggc gcaccagacc ctcgctgatg ccaatgcgtt cttgcacctc tttgggcatg
     2161 gaggcgtggg tcatgagcgc gggaagggaa atgagtgact cgacgccgcc gaggctttcc
     2221 gccagcgcgc acagccttac ctgattcaag aaccgccgcg ccgcttcgag gctgcccaag
     2281 tcaaacgaga gcatcgctcc cggcccgcgc tgttgtttgc gcgcgatatc gtgctgcgga
     2341 tgggacgcga ggcccgggta atacactttg cggactttcg cgtgggcatc gagatgccgc
     2401 gccacggcga tgccgttggc gttgtgttgc agcatgcgca cggccagcgt tttcaatccg
     2461 cgcgaaacca ggaagcaatc catcggcgcc agtcctgctc ccgccgagcg ctgaatgaag
     2521 tagatcttct cggtgtcctc gggccgcgtc agcaccacgc atccaccggt cgagtcgctg
     2581 tgcccgttca aatacttagt catcgaatgg accacgatgt gcgcgccgag ttcgatcggg
     2641 cgctgcaagt aagggcttaa gaatgtgttg tctaccacca acgtggcgtt gcgctcgctc
     2701 gccagtttgg ccatcgccgc aatgtcgctg atgcgcatgg tcggattggt cggcgtctcg
     2761 acatagagca tgcgcgtgtt ggcgcgcata gcggccagca ccgagtccga cgaagatgtg
     2821 tcgacaaaac tgaactccag cccgaaatga accagtagtt gtgtcgtgag gcggaaaact
     2881 ccaccataaa ccgcttccga aagcacgacg tgatcgccgg ggcgcaacaa acgaaacacc
     2941 gagtcgatcg ccgccatacc cgatgaaaac gcgtaggctg cgtgtccccc ttccagtttt
     3001 gccagcgtct tctccaacgc tttgcggttc ggatgattcg tccgcgcgta gtcgtagccc
     3061 ttgttcttgc ccaactcttc ctgcacgtac gttgatgtct ggtaaatcgg cgcgacgatc
     3121 gatccggtgg ccgggtccgg ctcctgcccg acgtgtatgg cgtttgtggc gaaacccatg
     3181 gctttctcct tgactgagcg gggacgtccc gtcaggcgac gcgatcaacg cacgcgatgg
     3241 aacggaattg aattgcgcgc gcccgtaaag gcgcgacttg ggagcttcct tgcagcggaa
     3301 gccgcacagt tcaaacaact tacaggaggc aatacgcgcc gtcaattgtc actccagacg
     3361 cttggttcac ggtttgcagc gcggccatgt ctttttcgga atccaattca gttgccagcg
     3421 atcgcccagc cgcacgccgc ttgacgccgc gccgccattt tgtggacgat gttagtggag
     3481 aagccatgaa atccacccgt ttcccggttg ccgcgcttgc tttgctcttc tttctcgcgc
     3541 ttccgctctg tgcgcaggac actccggcaa aggattcgca gtctccggac agcgcggacg
     3601 ccgctaccgc ggtcaagcgc ctgcgaattg aagtcaccgg tggcgaaaga aacgtggctg
     3661 ttgaaaacgc cagcgtctac ctgaagtacg tggaagaaa
//
LOCUS       DASTPM010000184         6951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1159611, whole genome shotgun sequence.
ACCESSION   DASTPM010000184 DASTPM010000000
VERSION     DASTPM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159611"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1471)
                     /locus_tag="VFR42_03235"
     CDS             complement(149..1471)
                     /locus_tag="VFR42_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368196.1"
                     /translation="MVPSPIRCLRNASLALALAAFFGTTLAQIPAKPAPAPAQKKTPP
                     SPPAPQSKHYPILLIATGTEPFWSLRIGMKGAERLERVGYPPIPLEPGEIEQEGTAEA
                     WLYHAKDAATTADVTVRVSREACSDNMSETKYSFHILVTHAQIGELKGCAKIAPDQFP
                     EFKQKNLDDDDPEKKKVTPPAITNFKPPVAVAYLDATGKVMLARGEAAKLVAPKGSQL
                     SLSHDGKRLLYTREDSPSERTIVLYDATTAKSTDLIRGAQSAFWSPDESQIAFLKLGD
                     GVFYVWTAPSNAPDKAAQLSNAPVVALHGWQDVHTVLATDSGKLYFLKTEAPPSSTDL
                     REIYGSSFQIAATDRLRASPVNSDLLAISAMAGATSNVFLYELRSKRRVPLTLPNLYA
                     ASPEWSRDGLQIFFTTRDAAKNSSIYRTLWDGSGQKRIRPGSDLVIGQ"
     gene            complement(1617..2480)
                     /locus_tag="VFR42_03240"
     CDS             complement(1617..2480)
                     /locus_tag="VFR42_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368197.1"
                     /translation="MGATDRFSRGEVQRILDVTEKQLEYWERLRIVSPQKGRGEKFYD
                     FRDLIGLRTAKQLIAAGVPANRLRRSLEALREKLSQITSPLAELRVVSDGRDIIVERD
                     GARLEPLSGQFVLNFETRELDDKVHVMPQRTADEWLAVARTYEAERQTWPDALYAYEQ
                     VLRIDPQRAEAVINSGTLQYEQGDLAAAAECFANAVALQPTDALAQFNLGSVLDESGR
                     LEDARHHLRQAVSLKPDYSDAHYNLAFVCEKLGAFEEARRHWRRYVELDPTSPWCEYA
                     RQRLGQNSAHA"
     gene            complement(2583..3443)
                     /locus_tag="VFR42_03245"
     CDS             complement(2583..3443)
                     /locus_tag="VFR42_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU0368198.1"
                     /translation="MASTVWKGHLTFGLISIPIRLFSAARGERISFNQLHSVCHSRVK
                     QPLYCPTCNRMVERSEIVKGYEYEKDQYVLFSEEELDKIEPVSAHTMEILEFVRADEV
                     DPLYLDASYYVNPEEAGLKAYQLLMKAMTDSGYAAIAKLTMHQREHIVIIRPGSKGLT
                     LHTMYYTNEVRAAEALPPAKVELKEAEKKLAQQLIESLAAPFDPVKYHDEYQDNVRKM
                     IDAKLKGQEITAVAQPQLAPVIDLMEALKKSIAEKQTTAQATAAKKPPARAVPELVPA
                     QKKSKKQQAG"
     gene            complement(3913..5676)
                     /gene="dnaG"
                     /locus_tag="VFR42_03250"
     CDS             complement(3913..5676)
                     /gene="dnaG"
                     /locus_tag="VFR42_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU0368199.1"
                     /translation="MAEAGSLAEKVKQQADIVRVISEYVQLKKAGQNFRGLCPFHAEK
                     TPSFNVHPTKQIYHCFGCGQGGDVFKFVMEMEKCSFPEAMRIVAEKCGIALPRPKERS
                     PEELRENQQRTVLVEIHREAQSWFVKQLEGTLEGKAARAYLEDRGLDRDTTTRFGIGY
                     APSGGDLLLRHLKAKHPEKSLVESGLISRDQSGRLFDRFRRRITFPIANESSKIVAFG
                     ARALGDDQPKYLNSPETPIYSKSTILHHLDRAKDALRRQDFAVLVEGYMDAIAVAHAG
                     ISNVVASCGTALAESQIKLLGRYTKRVIVNYDPDAAGQAATERSLTALLEQDFEVRVL
                     NLPSTGNKKADPDLFIREKGVDAYREQIKNSPPYVDYLITRARQMDLTTGEGKSRAVN
                     FLLPYVQRIPNSILRSEWATRIAQQLRIDEPVLRAALSKAAKERRSEVKLQPELVSRS
                     AKPAERRLIRMLAEAEGFRTELAQHLQASQLYHGLETEKIFAALVASGLSGNPFHASE
                     IGAMLDDRERRLFFEILFEDSQEPSWEEAQSCLDTLARKQLERELSEVQRSIEANPTG
                     QAMKDLLTRKQDLMRRLAASA"
     gene            complement(5722..6114)
                     /locus_tag="VFR42_03255"
     CDS             complement(5722..6114)
                     /locus_tag="VFR42_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU0368200.1"
                     /translation="MKNGVKDFTQLDAWKMARQLRSEIYKASKIFPRDETYALISQMR
                     RSAISVTANFAEGYLRYSVQENIQFCRQCRASAYELRDHCTAALDSGYISKKVFDELN
                     ALGISVIKLLNGYIRATMTGQQNAKKST"
     gene            complement(6132..>6951)
                     /locus_tag="VFR42_03260"
     CDS             complement(6132..>6951)
                     /locus_tag="VFR42_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEU0368201.1"
                     /translation="YLHRSREELDRMQQQMAAERSELEAERTKLRTEWAARQQKRIRE
                     LEAQFAEMQKRFEENIARVLEAVKERELRAQLEKTTRRKLGEVRSEAREELNAAVVQT
                     ISESQADLGVSPGSAAVVTAEQLQIGAKIRVRGFSKPVILRRIEGRNADIEAGPLRMK
                     IATDEIIGIEAPPAGKVAAPPSSIGAPNRSSATSEINVIGQTVEQATEQVDKFLDDAA
                     LAHLPSVRIIHGHGTGALRKGLSEFLRSHPLVAAHSAEAEERGGKAITVVELRT"
BASE COUNT         1350 a         1759 c         2268 g         1574 t
ORIGIN      
        1 aggaccagaa tattgaccgg actcggttcg ggctgagtag gggatcccat ttttgaagta
       61 taggcggccg ccgcatcgaa attgcgtggc cgtgattggc ggccgcttaa cgggcacctg
      121 tacgaaggga ctggcccgac cctaaaggct actggccgat gacgagatcg ctgccggggc
      181 gaatccgctt ctgccccgag ccgtcccaca aggtgcgata aatcgacgaa tttttggcgg
      241 cgtcacgagt cgtaaaaaag atttggaggc cgtcgcgcga ccattcgggt gaggcagcgt
      301 aaagattcgg caaggtcagc ggaacgcggc gcttggatct aagctcgtaa agaaaaacgt
      361 tgctggtggc gccggccatt gcggaaatcg cgagcagatc ggaattgaca gggctggcac
      421 gcaacctgtc cgtagccgcg atttgaaagc tggagccata gatctcgcga aggtcggtgg
      481 aggaaggtgg agcttctgtc tttagaaaat agagtttgcc ggagtcggtg gccaggacag
      541 tgtgaacgtc ttgccatccg tgcagcgcca caacaggagc attcgagagc tgcgccgctt
      601 tgtcgggagc gttggatggc gcggtccata cgtagaatac gccatcgccg agtttgagaa
      661 acgcgatttg ggactcgtcg ggcgaccaga aagcggactg cgcgccgcgg atcaagtcgg
      721 tggacttggc ggtcgttgcg tcgtaaagaa cgatagtacg ttcactcggc gagtcttcgc
      781 gcgtgtacag caatcgtttg ccatcgtgcg agagagaaag ctgcgagcct ttgggcgcga
      841 caagctttgc ggcttcgccg cgggcgagca taacttttcc ggtggcgtcg agataggcga
      901 ccgcaactgg cggcttgaaa ttagtgatcg ccggcggagt aactttcttt ttctcggggt
      961 cgtcatcgtc gagattcttc tgcttgaatt cgggaaactg atccggcgcg attttcgcgc
     1021 accctttcag ctcgccgatc tgcgcgtgcg tgacgaggat gtggaaggaa tacttggtct
     1081 ccgacatgtt gtcggagcac gcttcacggg aaacacgcac cgtgacatcg gccgttgttg
     1141 ctgcgtcttt cgcatggtag agccacgctt ccgcggtgcc ttcctgctcg atttcgcccg
     1201 gctcaagagg aatgggcggg tagccaacgc gttcaaggcg ttcggcgcct ttcatgccga
     1261 tgcgcaaact ccagaacggc tcggtgcccg tggcgatcaa aagaatcgga tagtgcttcg
     1321 attgcggggc gggaggggaa ggcggagttt tcttttgcgc cggtgctgga gccggcttgg
     1381 cgggaatttg cgccaacgtt gtgccgaaaa acgccgcgag cgcgagggca agggaagcgt
     1441 tgcgaagaca acggatcggg cttgggacca tggtcgccgt gcggagtcct tttgagagcg
     1501 cccgaggatt agaccaaatg gaagtagcga aaggcaaggt tgcaacgcaa aaagaacttc
     1561 gcatctcagg ctcatcctcg cactccccaa gaattttcga aatcagaaat tgcgggtcat
     1621 gcgtgcgcgg aattttggcc gaggcgctgg cgtgcgtatt cgcaccaggg gctggtggga
     1681 tccagttcca catagcggcg ccagtggcgg cgggcttcct cgaaagcgcc gagcttctcg
     1741 cagacgaagg cgagattgta gtgcgcgtcg gaataatcgg gcttgagcga aacggcctgg
     1801 cgcaaatggt ggcgcgcgtc ttccaggcgg ccggactcat ccagcacgct gcctaagttg
     1861 aactgtgcca gcgcgtcggt aggctgcaat gccacggcgt tcgcgaaaca ttctgcagcc
     1921 gccgcgagat cgccctgttc gtactgcagc gtgccagaat tgatgacggc ttcggcgcgc
     1981 tgcggatcaa tgcgcaacac ctgctcgtac gcgtaaagcg cgtcgggcca ggtttgccgt
     2041 tcggcttcgt aggtacgtgc aacggcgagc cattcgtcgg cggtgcgctg cggcatgacg
     2101 tgaactttgt cgtcgagttc gcgggtctca aagttcaaga cgaattggcc ggagagcggt
     2161 tcgaggcgcg cgccgtcgcg ttcgacgatg atgtcgcggc catcggaaac cacgcgcaac
     2221 tccgcgagag gtgacgtaat ttgcgataac ttctcgcgca gcgcttcgag cgagcggcga
     2281 aggcgattcg cgggaactcc ggcggcgatg agttgtttcg cggtgcgcag gccgatcaga
     2341 tcgcggaaat cgtaaaattt ttcgccgcgg cctttttggg gcgagacaat acgcaagcgc
     2401 tcccagtatt cgagctgctt ttccgtcaca tccaggatgc gctgtacttc tccgcggctg
     2461 aatcggtcgg tggcacccat ctagattgcc aaccctgtcg taagcttcaa aaacagttcc
     2521 tcaggggcta aagccccgct tgcttcgttc ctcaaatgcc ggagcttaag ctccgaccca
     2581 cactagccgg cttgctgctt tttgcttttc ttctgagccg gcaccaattc cggcactgcg
     2641 cgggccggtg gcttctttgc agccgtagct tgcgcggttg tttgcttttc cgcaatgctc
     2701 tttttcaagg cttccatcaa atcgatcacg ggcgcgagct gcggctgggc gacggcggta
     2761 atttcctggc ccttcagctt ggcgtcgatc attttgcgga cattgtcctg atattcgtcg
     2821 tgatatttga cgggatcgaa aggcgcggcc aggctttcga tgagctgctg cgcgagcttc
     2881 ttttcggctt ccttgagctc gaccttggcc ggcggcagag cttcggcggc gcggacttca
     2941 ttggtgtagt acatggtgtg cagcgtgagg cccttgctgc ccgggcggat gatgacgatg
     3001 tgttcgcgct ggtgcatggt gagcttggca atggcggcgt aaccggagtc ggtcatggcc
     3061 ttcatcaaaa gttgatacgc cttcaagccg gcctcctcgg gattcacgta ataagaagcg
     3121 tccaggtaga gcgggtccac ttcatcggcc cggacaaatt cgaggatttc catggtgtgg
     3181 gcggaaaccg gttcgatctt gtccagctct tcttcactga agaggacgta ctggtctttt
     3241 tcatattcgt agcccttgac gatttcagag cgttccacca tgcggttgca ggtcgggcaa
     3301 tagagcggct gcttgacccg cgagtggcag accgagtgga gctgattgaa gctgatgcgt
     3361 tcaccgcgcg cggcggaaaa aaggcgaatc ggaatcgaaa tgagaccgaa agtgaggtgg
     3421 cccttccaga ctgtcgaagc catgagctgc tccttagcgc gaggcgcttg caattgtcca
     3481 ggaacctaca agcgtagtcg cgacggcgag cggaagcaag attaagctcc atattggtaa
     3541 cgagatacga aagcgccgta ggggctgtaa agcccacccc ggtgtttttc ataagcggaa
     3601 attgaaggag ttagcaggca cgtcttaact gtggcaaaga agggacttac gttagggaga
     3661 tagggggtcg atttcagcta aaacgcggaa ggacttgtaa gtgcggagtt ctaaacggct
     3721 taaagcgggt tagcttgcag caattggcga ggcgaaatca gcggtttcgc gggaaagggt
     3781 cctgcgggct ggcaaccagt taacgcaaac cattatcgca tgtgtttacc gatcttcaca
     3841 tggcgatgtg aggtgctgga ccgttccgat atgggaaaag cctggcgccg ctggcgatcc
     3901 gcaaggaaaa ggtcaggccg aggcggctag acggcgcatc aggtcctgtt tgcgggtgag
     3961 gaggtctttc atggcctggc cggtggggtt ggcttcaata ctgcgttgga cttcgctaag
     4021 ctcacgctca agttgcttgc gagcgagggt gtcgagacaa ctttgcgcct cttcccagct
     4081 gggttcttgc gagtcctcga agagaatttc aaagaaaagg cggcgttcgc ggtcgtcaag
     4141 catggcgccg atttcggaag cgtggaaagg gttgccggaa aggccggagg cgaccagggc
     4201 agcaaaaatc ttttcggttt cgaggccgtg gtagagctgc gaggcttgca ggtgctgggc
     4261 gagttcagtg cggaagccct cggcttcggc aagcatgcga atgaggcggc gttcggcggg
     4321 cttggcggaa cggctgacga gttcgggctg gagtttgact tcgctgcggc gctctttggc
     4381 ggctttgctg agcgcggcac ggaggacagg ctcgtcaatg cgaagctgct gggcgatgcg
     4441 ggtggcccat tcggagcgga ggatgctgtt tgggattcgc tgcacgtaag gcaaaagaaa
     4501 atttacggcg cggcttttgc cttcgccggt ggtgagatcc atctggcgag cgcgggtgat
     4561 gaggtagtcg acgtagggcg gcgagttttt gatctgctcg cggtaggcgt caacaccttt
     4621 ttcgcgaatg aagagatcag ggtcggcttt cttattgccg gtggaaggca gattgaggac
     4681 gcgaacttcg aaatcctgct cgaggagcgc agtgagagac cgttcagtgg cagcctggcc
     4741 ggcggcgtcg gggtcgtagt tgacgatgac gcgcttggtg tagcggccca agagcttgat
     4801 ttgcgattcg gcgagagcag tgccacaact ggcgacgacg ttgctgattc cggcgtgggc
     4861 gacggcgatg gcgtccatgt agccttcgac gaggacggcg aaatcctgac ggcgaagcgc
     4921 gtccttggcg cggtcgagat ggtggaggat ggtgcttttg gagtagatcg gcgtttcggg
     4981 tgaattgagg tatttgggct ggtcgtcacc gagagcgcgt gcgccaaagg ccacgatttt
     5041 tgaggattca ttggcaatgg gaaaagtaat gcgacgacgg aaacggtcga agagacggcc
     5101 gctctggtcg cgggagatga ggccggattc gacgagagat ttttcggggt gttttgcttt
     5161 gagatggcgg aggagaagat cgccgccgct gggtgcgtaa ccgatgccga agcgcgtggt
     5221 ggtgtcgcgg tccaggccgc ggtcttcgag ataggcgcgg gcggctttac cttcgagagt
     5281 gccttccagc tgcttgacga accaggattg cgcttcgcgg tggatttcga cgagcacggt
     5341 gcgctgttga ttttcgcgca gttcttcggg tgaacgctct ttgggacggg gaagcgcgat
     5401 gccgcatttt tcggcgacga tgcgcatggc ctcgggaaac gaacattttt ccatttccat
     5461 tacgaatttg aagacatcgc cgccctggcc gcagccgaag caatgataga tctgcttggt
     5521 cgggtggacg ttgaaggacg gagttttttc cgcgtggaag gggcaaagtc cacggaaatt
     5581 ttgtccggcc ttttttagct ggacgtattc gctgattacg cggactatgt ccgcctgttg
     5641 cttgaccttc tccgctaatg agccggcttc agccatgtct tggaaaatgg aaaagagaaa
     5701 atggaaaatt ggcccgttgc atcaggtgct cttctttgcg ttctgttgtc cagtcattgt
     5761 cgcgcggatg tagccattca aaagcttgat cacagaaatg cctagagcgt tcaactcatc
     5821 aaatactttc ttggaaatat atcccgaatc aagcgccgcc gtgcagtggt cacgaagttc
     5881 gtaagccgaa gcgcggcact gccggcaaaa ctgtatgttc tcctggacgg aatagcgtag
     5941 atagccttcg gcgaagttgg cagtcacaga aatcgcggag cgtctcatct gagaaatcag
     6001 cgcgtaagtt tcatcccttg gaaagatctt actagctttg taaatctctg agcgcagttg
     6061 acgcgccatt ttccatgcat ccaactgggt aaagtccttg acgccatttt tcattttcat
     6121 gcttccatct tctaggtgcg caattctacg acagtgatgg ctttgccgcc gcgttcttcg
     6181 gcttcggcgg agtgtgcggc tacgagcgga tgggaacgga gaaattcgga aaggccttta
     6241 cgaagggcgc cggtgccgtg gccgtggatg atgcgtacgc tgggaagatg agctaacgcg
     6301 gcgtcgtcga ggaatttgtc tacctgttcc gttgcttgct ccacggtttg gccgatgacg
     6361 ttgatttctg aggtggccga ggaacggttc ggcgcgccta tagacgatgg cggggcggca
     6421 actttgccgg cgggcggcgc ttcgattccg atgatttcgt ctgtagcgat tttcatgcgc
     6481 agcgggccag cttcgatatc agcgttgcgg ccttcgatgc ggcgcaggat gaccggcttg
     6541 gaaaagccgc gtacgcggat ttttgcgccg atttgcagct gctccgccgt gaccaccgcg
     6601 gcggaacccg gcgaaacgcc gagatcggct tgcgattcgg aaatggtttg cactacggct
     6661 gcgttgagct cttcacgggc ttcgctgcgc acctcgccta gtttgcggcg ggtggttttt
     6721 tcaagttgtg cgcggagctc gcgctctttc accgcttcca ggacgcgcgc aatgttttct
     6781 tcgaaacgct tttgcatctc ggcgaactgc gcttccagtt cgcgaatgcg tttttgctgg
     6841 cgtgcggccc actcggtccg cagtttggtc cgctccgctt ccaattcgct gcgttccgcg
     6901 gccatctgct gctgcatgcg atcgagttct tcacggctgc ggtgaaggta g
//
LOCUS       DASTPM010000185         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_150298, whole genome shotgun sequence.
ACCESSION   DASTPM010000185 DASTPM010000000
VERSION     DASTPM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150298"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFR42_03265"
     CDS             <1..619
                     /locus_tag="VFR42_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0368202.1"
                     /translation="TTLRMLATILEPTGGTARLCGHDIVEHPERVRQHCGFLSTATAL
                     YPRLTAQEMVEYFGRLNGLDETTLKKRIDEIFQRLDMNGFCDRRCDKLSTGMKQKTSI
                     ARTLVHDPPVMIFDEPTLGLDIMTARTIIEFIRECRDRGKTVIFSTHVMREVEKLCDI
                     IGIIQNGKLLAEGTIEQLRQRHGTNDMEEIFVKIVTPHYQEGSLI"
     gene            616..1830
                     /locus_tag="VFR42_03270"
     CDS             616..1830
                     /locus_tag="VFR42_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368203.1"
                     /translation="MSMRNIGIVYRKELTEALRDRRTLITTFLVPLLLIPVLGAGFTG
                     VMSAVIGNAKKEKPRIMIIGGEDSPGVVEALRNNPKINVVPTSEDWKDLVVGKKIRAA
                     VEIPPSFQADLSKGQAKTVKINVYGGELKSEFAAANIESLLKEYRDSVATERLTANHL
                     PAELLKPFDVKRQNIAPPEKEAGAILGGIIAYMLILMCLNGAMHPAIDLTAGEKERGT
                     METILSSPVSRTHLVLGKFLLVMTASLITAVLLMVSVSVSSAVLQKSGALNQMVDEGE
                     PVPQLSLGPAAVASVIIMAVPLSVLFSAGLFTIALFAKSHKEAQSYIAPLMFLVVIPA
                     VSAMLPGVELTPKLAIVPLLNVSLLCKELVTGEYHWNFIILIFLSTCVYAAGALYLAV
                     KMFQRESVLFRS"
     gene            1881..>2175
                     /locus_tag="VFR42_03275"
     CDS             1881..>2175
                     /locus_tag="VFR42_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368204.1"
                     /translation="MARLIRIALILVFFPALLSAVVGWYGAAGFLHPEKRALTHDMTR
                     DADVTFAQIGAHREDFDVRAPDGTMLRGWKVHAAKPNGSCILVFHGVADNRYGM"
BASE COUNT          472 a          608 c          636 g          459 t
ORIGIN      
        1 caccacgctg cgcatgctcg cgacgatcct cgagccgacc ggcggaacgg cacggctctg
       61 cgggcacgat atcgtggagc atccagagcg tgtgcggcag cattgcgggt tcctttcgac
      121 ggcgacggct ttgtatccgc ggctgacggc gcaagaaatg gttgagtact ttgggcgtct
      181 gaacgggctt gacgagacaa ctctcaagaa gcgcatcgat gagatttttc agcggctgga
      241 tatgaacggc ttttgcgacc ggcgctgcga caagctttcc actggtatga agcagaagac
      301 ctccattgca cggacgctgg tgcacgatcc accagtgatg atcttcgacg agccgacgct
      361 ggggctggac atcatgacgg cgcggaccat tatcgagttc attcgcgaat gccgcgaccg
      421 gggaaaaacc gtgatttttt cgacgcacgt gatgcgcgag gtggaaaagc tttgcgacat
      481 cattggaatt atccagaacg gcaagctgct ggccgaaggc accatcgaac aactgcgcca
      541 acggcacggc acaaacgaca tggaggagat cttcgtaaaa attgttacgc cacactacca
      601 ggagggtagt ctgatatgag catgcgcaat atcggcatcg tctaccgcaa ggaattgacc
      661 gaggccctgc gcgaccgccg cacactaatc acaacatttc tggtaccact gctgctgatt
      721 cccgtcctcg gcgcgggatt cacgggcgtg atgtccgcag tgatcggcaa cgcgaaaaaa
      781 gagaaaccga gaatcatgat catcggcggc gaggactcgc ccggcgtcgt ggaagcgctg
      841 cgcaataacc caaagatcaa cgttgtgccg accagtgagg actggaaaga cctggtggtc
      901 ggaaagaaaa tccgcgccgc ggtcgaaatt ccccccagtt ttcaggccga cttgtccaaa
      961 ggccaggcga agacggtcaa gatcaatgtt tacggcggcg agttgaagtc cgaatttgcg
     1021 gcggccaaca tcgagagctt actgaaggag tatcgcgaca gtgtcgctac agagcgcctc
     1081 acggcgaacc atctgcccgc agagttgctg aaacctttcg acgtgaagcg gcaaaatatc
     1141 gcgcccccgg aaaaggaggc tggcgccatt ctaggcggaa tcatcgccta catgctgatc
     1201 ttgatgtgcc tgaacggcgc gatgcatccg gcgatcgatt tgaccgccgg cgaaaaggag
     1261 cgcggcacca tggagacgat cctgtcgagc cccgtttcgc gcacccacct ggtgctggga
     1321 aaattcctgt tggtaatgac cgcttcgctg atcaccgcgg tgctgttgat ggtctccgta
     1381 agcgtctctt cggcggtcct gcaaaagtcg ggcgcgttga atcaaatggt tgatgaaggc
     1441 gaaccagtgc cgcaactgtc gctcggaccg gcggcggtcg cctcggtgat catcatggcg
     1501 gtaccgctgt cggtactttt ttcggctgga ctcttcacca ttgcgctgtt cgccaagagc
     1561 cacaaggaag cgcaaagcta catcgctccg ttgatgtttt tggtagtgat tccggcggtg
     1621 tcggccatgc tgcccggcgt ggagcttacg cccaaactcg ccatcgttcc gctcctgaac
     1681 gtcagcctgt tgtgcaagga gctggtgacc ggcgaatacc actggaactt cattattctg
     1741 atctttcttt ccacctgcgt gtacgcagca ggcgcactgt acctggccgt caaaatgttc
     1801 cagcgcgaat cagtgctgtt tcgttcctag cgcgccttct tttttagaaa cattggacgc
     1861 agccgacgcg tatctattgc atggcccgcc tgatacgcat tgccctgatt ctggtgttct
     1921 ttcccgcatt gctttctgct gtcgtcggct ggtatggcgc cgcgggcttt ttgcatcctg
     1981 aaaagcgagc gctcacacac gacatgacgc gtgacgcgga cgtaactttt gcgcagattg
     2041 gagcgcaccg cgaagatttc gacgtacgag cgccggacgg cacgatgctg cgcggctgga
     2101 aagtacacgc cgcaaaaccc aacggttcct gcatactggt atttcatgga gttgccgaca
     2161 atcgttacgg aatgg
//
LOCUS       DASTPM010000186         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_92880, whole genome shotgun sequence.
ACCESSION   DASTPM010000186 DASTPM010000000
VERSION     DASTPM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92880"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFR42_03280"
     CDS             <1..346
                     /locus_tag="VFR42_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0368205.1"
                     /translation="LLAVAGVYGVVAYGVTQRTREIGMRIALGANSADVLRLILRQGL
                     TTTLIGVAIGVAGSLATARTIQSLLFGVKPTDPLTFIAVAASLVAVAGLACYIPARRA
                     TKVDPMVALRYD"
     gene            405..653
                     /locus_tag="VFR42_03285"
     CDS             405..653
                     /locus_tag="VFR42_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU0368206.1"
                     /translation="MIRGTIEMPIYEYVCDACETRFEKIVINKRQEIACPKCASKKAS
                     IQLSVFATANGSSNGSSAKSSGPSGGGGSCCGGGCGCH"
     gene            complement(796..1188)
                     /locus_tag="VFR42_03290"
     CDS             complement(796..1188)
                     /locus_tag="VFR42_03290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEU0368207.1"
                     /translation="MADARFAALAMHKYLNLETFRKNGQGVRTPVWFAGEPELGTPEK
                     VYVYSTESSGKAKRIRNSGRARVAPCTGRGKLLGEWMEARAEIVSGAEAEHGTKLLNT
                     KYFPLKQILGFFALFSRQKRVVFLIRPA"
     gene            complement(1201..1653)
                     /locus_tag="VFR42_03295"
     CDS             complement(1201..1653)
                     /locus_tag="VFR42_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368208.1"
                     /translation="MKKKKNQKKKATSKSRVSAKAKLRAKSAAKGKSAKKKAASPRAR
                     VAPMGRIRESQDVMENAGVRKVPMSAVRPKARSARVGGGAGDYEGISRVESVDSESPE
                     ELLEEGNAFEAGIVEGVQNAPNANRGEVRTREVPEDDVPTEYDDQDRP"
     gene            complement(1719..>2024)
                     /locus_tag="VFR42_03300"
     CDS             complement(1719..>2024)
                     /locus_tag="VFR42_03300"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0368209.1"
                     /translation="TAEWADELPESAVAEAGAVNIYVLHDVHALDLNARAAGFHVVVS
                     GHSHKPGRSERDGVVYINPGSAGPRRFDLPITVARLDLSTTPWAVEFLDLEKGRRVK"
BASE COUNT          369 a          698 c          515 g          442 t
ORIGIN      
        1 gctgctggcc gttgcggggg tttatggggt ggtggcgtac ggcgtcacgc agcgaacccg
       61 cgagatcggc atgcgtatcg cgctcggcgc caattcagcc gatgttctgc gcctgatcct
      121 gcgtcaggga ttaacgacca cgctcatcgg agtggccatc ggggtcgccg ggtcgctggc
      181 cacagctcgc accattcaat cattattgtt cggtgttaag cccaccgatc cgctcacatt
      241 tattgccgtg gcggcatccc tggtcgccgt tgccgggctg gcctgctata tccccgcgcg
      301 gcgcgccact aaagtggatc ccatggtggc cttgcgatac gactgagtac gccggacgat
      361 tcgggaagcg ctgcgctctg ctataattta gctgaaaacg ctgagtgatt cgaggaacaa
      421 tcgaaatgcc catctacgaa tacgtttgcg acgcctgcga aacccgtttc gagaaaatcg
      481 ttattaacaa gcggcaggaa atcgcctgtc ccaagtgcgc cagcaaaaaa gccagcatcc
      541 agctttccgt cttcgccacc gccaacggtt ccagcaacgg cagctccgca aaatcttccg
      601 gcccctccgg cggaggcggc tcctgctgcg gcggcggttg cggctgccac tagtagcaca
      661 gccactcttg gctgtgcagc gtcaacccca gcaacccatt ccgcgaaacg ccacctgacg
      721 tgatagcggc aaagaatccg gttgtccggt tcaatcttta aattattcca cgcgagattt
      781 gtacgatcga gagaactacg ccggccggat cagaaacacc acacgcttct gccgtgaaaa
      841 cagtgcgaaa aaccccaata tttgcttgag cggaaaatac ttcgtattca gcagcttcgt
      901 cccgtgttcc gcttccgccc cgctgacaat ttccgcgcgc gcctccatcc attcgccgag
      961 caattttcca cgcccggtac aaggcgccac gcgcgcacgt ccactattac ggatgcgctt
     1021 ggccttgccg gaactctcgg tgctatacac atacactttt tccggcgtgc ctagctccgg
     1081 ctctcccgca aaccacaccg gagtccgcac accctgccca ttcttgcgaa acgtttcgag
     1141 attcaaatac ttgtgcatcg caagcgcagc aaatcgcgcg tccgccataa cggcattccg
     1201 ctacgggcgg tcctggtcgt cgtactccgt cggaacgtcg tcctcgggga cttcgcgggt
     1261 gcgtacttcg ccgcggttcg cgttcggcgc gttttgcacg ccttcgacga tgccggcttc
     1321 aaaggcgttg ccttcttcca aaagttcctc cgggctttcc gaatctacgc tttccactcg
     1381 ggaaattccc tcgtagtcgc ctgcgccgcc gcccacgcgc gccgacctcg ccttcggccg
     1441 caccgccgac atcggcactt tgcggacgcc cgcattttcc atcacatcct ggctctcgcg
     1501 gatgcgcccc atcggtgcaa cgcgcgcccg tggactcgcc gcctttttct tcgcactctt
     1561 gcccttcgcc gcgctcttgg cgcgcaattt tgctttcgcc gaaacccggc tcttgctggt
     1621 tgcctttttc ttctgattct tctttttctt catcgtcgcg ctccgttatg gcgccgcttt
     1681 gttgccagaa caagacaagg ccgttcgaac cgccgcgcct acttcactct acgccctttt
     1741 tccaaatcca gaaactccac cgcccacggc gtcgtgctca ggtccagccg tgccaccgtg
     1801 atcggcaaat cgaaccgccg cggccccgcg ctccccggat tgatgtacac aactccatcg
     1861 cgctcgctgc gccccggctt gtgggaatgc ccgctcacca caacgtgaaa tccggcggcc
     1921 cgcgcattca gatctagagc atgcacatca tgcaacacat aaatgtttac cgcgcccgct
     1981 tccgccactg cggactccgg caactcatcg gcccactcgg cagt
//
LOCUS       DASTPM010000187         3667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1332521, whole genome shotgun sequence.
ACCESSION   DASTPM010000187 DASTPM010000000
VERSION     DASTPM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332521"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..645
                     /locus_tag="VFR42_03305"
     CDS             304..645
                     /locus_tag="VFR42_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0368210.1"
                     /translation="MTTPRGEVLQGTLDLIVLKTLDALGPLHGYGIAQRIQQVSEDLL
                     KLNQGTLYPALLRLEQRGWISSKWGVSDNNRKAKYYSLTRSGRKQLATEAESWERMAG
                     IIARLLGAEGA"
     gene            647..3346
                     /locus_tag="VFR42_03310"
     CDS             647..3346
                     /locus_tag="VFR42_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368211.1"
                     /translation="MSRLHIFRSRLLALFRKRSSDENLETELQAHLEALTQENIRRGM
                     TPDEARHAALREFGGLEQTKETYREQRGLPFLETLSQDVRFGARLLRKEAALALVIVA
                     ILAIGIGAGTSIYSLIDACLVRSITYPVVDRWVAVHAYLPGQKSFVNYMSVPEMLEVK
                     QLHELFEDVGSIHGDGFTLTEGQYPERVAGTWVSANAITMTHVAPILGRVFREDEDRP
                     GGPRVVVLSYELWQRRFAGDRNILGRVMRLNDLDYTVIGVMPRYYGLWGGELWIPVQL
                     DLGNSNRSDRRNWIIAVLRKGVTEAQANARLHALSKQLEQQYGITTPEYRDWDLSVWN
                     IREAINGAVKPALLVLAGAVGLLILVVCANVAVLLLARATSRLKEVALRTALGAGRAR
                     LLRQMLTESLLLAFTGAVAGICVSIACLPLLVHLIPSEYLNTEPELVRVDHNAIAVAC
                     AIAAFMGILFGIVPALQVSKQDFVETLKQGGSKIGGSRFGRFTRNALITVEIALSLVV
                     LAAAVLMAQSYRRLEGIDLGFRADHLLTFELSLPDTRYGLADQITNFFSRALQEIRSS
                     PGVVSVAAASGRPMGERTSDLLSRDFSIEGRPSEDARSAENAVFRVISPEYFQTMGAR
                     ILQGRSFSEQDGRGAPLSAIVNQALARQYWPAGDAVGHRIRLGRQYGRREAFSGSDAE
                     EPSLMIVGVVSDVRQVRVIDAPIRQELYVPLAQQTNPPRIMNVLVRSTMEPSSLADSA
                     RAAIRRVDAEQPIYGVTTMDQLVADSFGPKRLTLFLLVFLSAIVLLLACTGLYAALSY
                     SVGQRRREIGIRMAVGAKSRDILRLIVSEGAFLAVRGVALGLLAAVALTRLMQTLLYQ
                     VAASDPLTLFGVAVILVAVAMIASYLPARRAARVDPTAVLRSE"
     gene            complement(3368..>3667)
                     /locus_tag="VFR42_03315"
     CDS             complement(3368..>3667)
                     /locus_tag="VFR42_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368212.1"
                     /translation="RFLAEHAQGKTQILEKGVEERQAALVSIGFFGLLEAAKFAARGL
                     ACLLSGHAAAEIFFFQQLQMRAELGVEGLLIARPCENRKKPFCQRLDVVHCSILA"
BASE COUNT          679 a         1150 c         1075 g          763 t
ORIGIN      
        1 ctcttcagcc cttgcgctgg actttttgcg gccagcgcgg tcagaaagca gagcgtaacg
       61 gccatcccta gcaccgtgct tcgcttcggc atacgcataa gaatcccctt tcgccacgga
      121 caggcgtgaa ttggcccggc catgctaggc cgcggaaagc cctcgggtca acctgggcaa
      181 cagcggtcct ccatcagcac agaaacattt cttccggtcc gtcgtctaat atgtgagacg
      241 cgacctgttg acaatcaaca ggagcgcagt ttagcctcct gttgatcgcc taaaggaact
      301 actatgacca cgccgcgcgg cgaagttctg cagggaacgc tggacctgat cgtcttgaag
      361 acgctcgacg ccctcggccc gctgcacggc tatggcatcg cccagcgcat ccagcaagtc
      421 tcagaagatt tgctcaagtt gaaccagggc acgctgtatc cggcattgct gcgcctcgaa
      481 cagcgcggct ggatcagctc caagtggggc gtctccgaca acaatcgcaa agccaaatat
      541 tactcgctga cccgctctgg gcgaaaacaa ctggccaccg aagcggaaag ctgggagcgc
      601 atggctggaa tcatcgcccg cttactcggc gccgaaggtg cgtgagatga gtcgcctgca
      661 cattttccgt tcgcgcctgc tcgctttatt tcgcaaacgc agcagcgatg aaaatctcga
      721 aaccgagctg caggcgcacc tcgaagccct gacgcaggaa aatattcgcc gcggcatgac
      781 cccggatgaa gcacgccacg ccgcgctccg cgaattcggc ggcctggagc aaaccaagga
      841 aacctaccgt gagcagcgcg gactgccttt tctcgaaacg ttgtcgcaag acgtgcgctt
      901 cggtgcgcgg ttattgcgca aggaagccgc cctcgctctg gtcatcgtcg ccatcctggc
      961 catcggcatc ggagccggca catccatcta cagcctgatc gatgcctgcc tggtgcgcag
     1021 catcacctat ccggttgtag accgttgggt ggcggtccat gcctacctgc ccggccagaa
     1081 atcgttcgtc aattacatgt ccgttccaga gatgctcgag gtcaagcagc tgcatgaact
     1141 ttttgaagat gtaggttcaa tccatggcga cggcttcacg ctgaccgaag gccagtatcc
     1201 ggaacgcgtg gccggcacct gggtatccgc caatgccatc accatgaccc atgtagctcc
     1261 catcctgggc cgcgttttcc gcgaggatga agaccgcccc ggcggtccgc gcgtggtggt
     1321 gctcagctac gagctgtggc aacgccgctt tgccggcgac cgcaacatcc tgggccgcgt
     1381 gatgcggctc aatgacctcg attacaccgt catcggagtg atgccgcgtt attacgggct
     1441 gtggggcggc gaactgtgga ttccggtcca acttgatttg ggaaacagca atcgttccga
     1501 ccgacgcaat tggatcatcg ccgtgttgcg aaaaggcgta accgaggcgc aggccaacgc
     1561 ccgtttgcac gctctttcca agcaactcga gcagcagtac ggcatcacca cgccggaata
     1621 tcgcgattgg gatctgagcg tctggaacat tcgggaagcg atcaatggcg cggtcaagcc
     1681 ggcgctgctg gtgctggccg gcgcggtcgg attactgata ctggtggtgt gcgccaacgt
     1741 ggccgtgctg ctgctcgctc gcgctacatc gcggctcaag gaagtggcct tgcgcaccgc
     1801 gctcggcgcc ggccgggcac gcctgcttcg tcaaatgttg acggaaagcc tgttgttggc
     1861 gttcaccggc gcggtcgccg gcatttgtgt atccatcgcg tgtctgcccc tgctggtcca
     1921 cttgattccc agcgaatacc tgaacaccga accggagttg gtgcgcgtcg atcacaacgc
     1981 cattgccgtg gcctgcgcaa tcgccgcgtt catgggcatc ttgttcggca tcgtgcccgc
     2041 gctgcaggtc tccaaacagg acttcgtcga aacgctgaag cagggcggct caaaaatcgg
     2101 cggcagccgc tttggccgct tcactcgcaa tgcactgatc accgtggaga ttgccttgtc
     2161 cctggtggtg ctggccgccg cggtgttgat ggcccagagc tatcgccgcc tggaaggcat
     2221 tgatctgggt tttcgtgcgg atcacttgct gacgtttgag ctttccttac cggacacccg
     2281 atatgggctc gccgatcaaa tcaccaattt cttttcgcgg gccctgcagg aaattcgctc
     2341 ctcaccgggg gtggtcagcg ttgccgctgc gtccgggcgt ccgatggggg aacgcaccag
     2401 cgatctgctc tcccgcgact tcagcatcga ggggcgcccc agcgaagatg cgcgctcagc
     2461 ggaaaatgcc gtcttccggg taatcagccc ggagtatttt caaactatgg gtgcgcggat
     2521 cctgcaaggc cgcagctttt ccgagcagga tggccgtggc gctccgcttt ccgcgatcgt
     2581 caatcaagcc ctggctcgcc agtactggcc ggcgggagat gcggtcggtc atcgtatccg
     2641 gctcggcagg caatacgggc ggcgcgaggc cttttccggt tccgatgcgg aagaaccttc
     2701 tttgatgatt gtcggcgtcg tctccgatgt gcgccaggtt cgcgtgatcg acgcgcccat
     2761 tcgccaggaa ctatacgtgc ctctggcgca gcagaccaat ccgccccgca tcatgaatgt
     2821 tttggtgcgc agcacaatgg aaccctcttc gctggccgat tcagcacgcg ccgccatccg
     2881 cagagtcgat gctgaacagc caatctatgg cgtcaccacc atggaccagc tggtggccga
     2941 ctccttcgga ccgaaacgcc tgacactttt cctgcttgtc ttcctttccg cgattgttct
     3001 tttgctggct tgcactggtt tatacgccgc gctttcctat tccgtcggac agcgccgccg
     3061 cgagatcggc atccgcatgg cggtgggcgc gaaatcccgc gacattctgc gcctgatcgt
     3121 ttctgagggc gcgttcctgg ccgtgcgcgg cgttgcgttg gggttgctcg ccgccgtcgc
     3181 gctcactcgt ctgatgcaaa cgcttctgta tcaggtcgcc gcttccgatc cgcttacgtt
     3241 gttcggcgta gcggtgattt tggtcgccgt ggccatgatc gcaagttatc tccctgcgcg
     3301 ccgcgcggcc cgtgtcgacc ccaccgccgt gctgcgcagc gagtgacgcc atgcaaacgt
     3361 ttgagattca cgcgaggatt gagcaatgaa ctacatcaag acgttggcaa aacggctttt
     3421 ttctgttttc acaaggacga gcaatgagca atccctcgac gccgagctcc gcacgcatct
     3481 ggagctgctg gaaaaagaaa atctccgccg cggcatgacc gctgaggagg cacgccaggc
     3541 cgcgcgccgc gaatttggcg gcctcgagca aaccaaagaa gcctatcgag accagcgcgg
     3601 cctgcctttc ttcgacgccc ttctccagga tctgcgtttt gccttgcgca tgctcggcaa
     3661 gaaaccg
//
LOCUS       DASTPM010000188         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_496559, whole genome shotgun sequence.
ACCESSION   DASTPM010000188 DASTPM010000000
VERSION     DASTPM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496559"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFR42_03320"
     CDS             complement(<1..579)
                     /locus_tag="VFR42_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368213.1"
                     /translation="MRVLLEIFGQTLSTLWAHKLRSLLTMFGIAWGVGSLLLLVGLGE
                     GFRSGNKKQFDSLGENVMFIWGGRAPAVEGSSTGMRQYYLTYKDYLDIVADCPDVKAA
                     VPAISRQDLRSVSDFFQSSGQLLGVPASFNQIRFVPIREGRWLNDLDDAQRRHVIVLG
                     DEARRILFPGRPSTGSSIMINGIRYQVIGTLLP"
     gene            746..1174
                     /locus_tag="VFR42_03325"
     CDS             746..1174
                     /locus_tag="VFR42_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368214.1"
                     /translation="MRLTVLCLLSFGPMLMPRHASAAACPNLSVHPGVTTVFIQSDFD
                     EKDAAAKAYVFGLQEAVKKSKTFCPVEDLRAATFAVDVAGVDLNEDHERAAFSVVIVS
                     EKGTMISHWVRLSSVDNVEKNATDDVVKLERVVQKAKRKG"
     gene            1281..2462
                     /locus_tag="VFR42_03330"
     CDS             1281..2462
                     /locus_tag="VFR42_03330"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEU0368215.1"
                     /translation="MPWRECSVMDERMQFVARRLAGEPMAELCREFGISRKTGYKLFD
                     RYQECGLQGLTDRSRRPYRYANQLPFQVENFILNMKREHPSWGARKIRERLLRRFSGI
                     PIPAKSTVHAVLDRHGLVERRARVRRRVQGTSLSLGQRPNDLWCTDYKGEFLLGNRQY
                     CYPLTVTDHASRFLLTCEALSSTKENYAFAVFERLFKDRGLPANIRSDNGVPFASAHA
                     LFHLSKLAVWWLRLGIGIERIQPGHPQQNGRHERMHLTLKKEATKPAAANLLQQQARF
                     DKFIEVFNHQRPHEALNMKCPAEIYQPSPRPYTGLPDIDYPFHDKTIVVTRCGRICLG
                     NRKINFSQVFAGQAVGIKEMHDDIWLVSFMDYDLGYFDLETRVLEPLENPFGPKVLPM
                     S"
BASE COUNT          591 a          785 c          694 g          553 t
ORIGIN      
        1 gggcagcaac gtgccgatta cctgatagcg aattccattg atcatgatgc tgctgccagt
       61 cgatggcctc cctggaaaca ggattcggcg cgcctcatct cccaacacga tcacgtgtct
      121 ccgctgcgcg tcatcgagat cgttaagcca gcgcccctca cgaatcggta cgaaccggat
      181 ctggttgaaa ctcgcgggca cgcccaacaa ttggccgctg ctttgaaaga agtcgctgac
      241 cgagcgcagg tcctgacgcg aaatcgccgg aacggctgcc ttcacgtccg ggcagtccgc
      301 aacaatgtcg aggtaatcct tgtaagtgag atagtactgg cgcatgccag tggagctgcc
      361 ttccaccgca ggcgcccggc ctccccagat gaacatcacg ttctcgccca gagaatcgaa
      421 ctgtttcttg ttccccgacc gaaagccttc gcccaatcca accagcagca agagcgaacc
      481 gacaccccac gcgatgccga acatggtcag caatgagcgc agtttgtgcg cccacagcgt
      541 gctcaacgtc tgaccaaaaa tttccaacag gactcgcatg cttacctttg atcataattc
      601 atggctcgga gtgtcgtcgg tgacgactaa taactgtttt cgatgaactg ggagagccgc
      661 gaagcttgga tagaattgat aatgtttggc ttgtgttcct agagacccgt ttcacaattc
      721 atcctgtccg cgtacggagg ccttcatgcg tttaacagtc ttatgcctgc tttcattcgg
      781 gcccatgctg atgcctcgcc acgcgagtgc cgccgcttgc ccgaacctca gcgtccatcc
      841 cggcgtcacc accgttttca ttcaaagtga ttttgacgaa aaagacgctg ccgccaaagc
      901 ctacgtattt ggcctgcagg aggcagtgaa gaaatccaaa accttctgcc ccgtggaaga
      961 cctgcgcgcg gcaacctttg ccgtcgacgt cgccggggtc gacctcaacg aagaccatga
     1021 acgtgccgct ttcagcgttg tcatcgtctc ggaaaagggc accatgatca gccattgggt
     1081 tcgtctgtcc tccgtcgaca acgtcgaaaa gaatgctacc gacgatgtgg ttaagcttga
     1141 gcgcgttgtg cagaaggcga aaagaaaggg ctaggcccaa tacttacaat gcaaagcaag
     1201 ggctgagcgg cctgagacat aggtaacaga ttgtaccgga cacataggtg acacttcctc
     1261 cctttcgggg aggagtgaaa atgccttgga gggagtgttc ggtgatggat gaacggatgc
     1321 agtttgtggc tcgccggcta gctggcgagc cgatggcgga actttgcaga gagtttggga
     1381 tttctcgaaa gaccggctac aagctctttg atcgctatca ggaatgtggc cttcaagggc
     1441 tgaccgaccg cagccgccgc ccatatcgct atgccaacca gctccccttc caggtggaaa
     1501 atttcatctt gaacatgaag cgcgagcacc ccagctgggg tgctcgaaag attcgcgagc
     1561 gcttgctccg cagattctcg ggcattccga tcccggccaa aagcacggtc catgccgtgt
     1621 tggatcgcca cggcctggtc gagcgccgcg ctcgcgtgcg tcgtcgtgtg caaggcactt
     1681 cgttgtctct gggacagcgg cccaacgatc tctggtgcac cgactacaaa ggcgagttct
     1741 tgctcgggaa tcgccaatac tgctatccgc tgaccgttac cgaccacgcc agccgctttc
     1801 tcctcacttg tgaagcgctt tcttccacca aggaaaacta cgctttcgct gtatttgagc
     1861 ggctgtttaa agatcgtggc ttgcccgcca acatccgcag cgacaacggc gtgcccttcg
     1921 cttccgcaca cgccttgttc catctgagca agctcgcggt ctggtggtta cgtctcggca
     1981 ttggcatcga acgcatccaa cctggccatc ctcagcaaaa cggtcgtcac gaacgcatgc
     2041 acctcacttt gaagaaagaa gccaccaaac ccgccgccgc caatctcctg caacagcagg
     2101 cccgcttcga caagtttatc gaggtcttca accaccaacg tcctcacgaa gccctgaaca
     2161 tgaaatgccc ggcggagatc taccagcctt ccccacgtcc ctacaccgga ttacccgaca
     2221 tcgactatcc ctttcacgac aaaaccatcg tggtcacgcg ctgcgggcgc atctgcctgg
     2281 ggaacaggaa aatcaatttc agccaagtct tcgccggaca agccgtgggc atcaaagaga
     2341 tgcacgacga catctggctg gtgagcttta tggattatga tctgggatat tttgatctgg
     2401 agacgcgggt gctcgaaccg ctcgaaaacc cgttcggccc aaaagtgtta cctatgtctt
     2461 aggtacgatc tgtcacccat gtctccgggc cggaccgaaa attgttggtc gggacgggca
     2521 gatttgaact gccgaccctc gcacctcaag cgagattggc accaagtggc agccctgctc
     2581 gggaagcaag cggttaagcg agtatcactc caggcgcgca agg
//
LOCUS       DASTPM010000189         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1275051, whole genome shotgun sequence.
ACCESSION   DASTPM010000189 DASTPM010000000
VERSION     DASTPM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275051"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..559)
                     /locus_tag="VFR42_03335"
     CDS             complement(131..559)
                     /locus_tag="VFR42_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0368216.1"
                     /translation="MIRRGESYTLPRNMSRVEALEFWFAPGHETFVFEESGVVLGTYF
                     LRANQGGGGSHVANCGYVTAETAQGRGIARTMCLHSLERAKERGFRAMHFNFVVGTNE
                     RAVKLWLSLGFEIVGRLPGAFRHPENGFVDALVMYKRLCE"
     gene            complement(632..>2506)
                     /gene="ligA"
                     /locus_tag="VFR42_03340"
     CDS             complement(632..>2506)
                     /gene="ligA"
                     /locus_tag="VFR42_03340"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU0368217.1"
                     /translation="ETAHPQLVTPDSPTARVGAPPRAGFQTVKHTRPMLSLDNAFSFD
                     ALRDFDRRVREGVGREEIEYIAEHKFDGLSISLIYEDGVLARGVTRGDGTTGEDVTPN
                     VRTIRSIPLRIALETLKKAKMVGSFEVRGEIIMTRKAFEAMNRQQEQSGGKIFANARN
                     AAAGAVRTLDSSVTAQRKLDFFAYYLLRDGRVAFPKHSESLEALNLLRFRASSDWKLC
                     DGIDEVIKYCGAWDSKREKLPYEIDGVVVKVNSVAIQNELGFTSKSPRWAIAYKYPAR
                     QETTVVNDIQVNVGRTGVLTPWTLLQPVQIGGVTVSRSTLHNMDEIERLGVQIGDTVL
                     VERAGEVIPHVLKVVKEGKDRRPFRMPKNCPECGSKIHRVEGEVAYRCLNAACPAKRR
                     ESLLHYASRHAMNIDGLGEKIVDQLVEKDMVKDVADLYSLKLEEVAELERMAEKSAQN
                     LLDEIESSKKNSLSRLIYALGILFVGERTGQLLAEHFSSLDELAEATQEQLEEVPEVG
                     PKVANSIVQFFSEPANRQLIKKLEKAGVRPTAEKREVKSDKFAGKSFVFTGGLANRSR
                     EDAGELVKQHGGKVASSVSKKTDYVVVGTDPGSKFDKAKELSVPILSEAEFETLIGLK
                     "
BASE COUNT          512 a          824 c          602 g          568 t
ORIGIN      
        1 ctgcgccact ctgtgcgcga gacccagagc tgagccgata tccgacaacg acggatagtg
       61 ctcggcacaa aattgaaacg ccgcccaaac cacacctact agaatgatcc ctctagcaac
      121 tcctacgatt tcattcgcac aaacgcttgt acatcaccag cgcatcgaca aacccattct
      181 ccggatgcct aaatgctccc ggcaggcgcc cgacaatttc aaagcccagg ctgagccaga
      241 gcttgacggc ccgctcgttc gtgccgacta cgaaattaaa gtgcatggcg cgaaacccac
      301 gctccttcgc acgctccaga gaatgcaggc acatcgtccg ggcaatcccg cggccctgcg
      361 ctgtctctgc cgtcacatag ccgcaattcg caacgtgcga tccgcccccg ccttgattag
      421 cccgcaagaa gtacgtcccg agtaccacac cactttcttc gaacacgaaa gtttcatgtc
      481 caggtgcaaa ccaaaactca agtgcctcga ctctgctcat gttgcgcgga agcgtatacg
      541 actcgccgcg gcggatcata ggctctagga tcgcccagat cgactcttgg tcctgctccg
      601 tcgctacccg aatcgccaaa ctgagcttcc ttcacttcaa tccgatcagt gtctcgaatt
      661 cggcttcgga cagaatcgga acgctgagct cttttgcttt gtcgaatttc gatcccgggt
      721 cagtgcccac gacgacataa tccgtttttt tgctcacgga actggctacc tttccgccat
      781 gttgctttac gagttcgcct gcatcctcgc gcgaacgatt agccagcccg ccagtgaaca
      841 cgaacgactt tcctgcgaac ttatcgctct tcacctcgcg tttttcggca gtgggccgca
      901 cgccggcctt ttcgagcttc ttgatgagct gccggttcgc cggttccgaa aaaaactgca
      961 cgatgctgtt ggcaaccttg gggccaacct ccggcacttc ttccagttgc tcctgtgtgg
     1021 cctcggccaa ttcgtcgagt gaggaaaaat gctctgccag caattgtcca gtgcgttcgc
     1081 ctacgaaaag aattcccagc gcgtaaatca gccgagacaa cgaattcttt ttgctggact
     1141 cgatctcatc cagtaagttt tgcgcggact tttccgccat tcgctccagc tccgccacct
     1201 cctccaactt cagcgaatac aggtcggcga cgtccttaac catatctttc tcaacaagct
     1261 ggtccacgat cttttcgccc agaccgtcaa tattcatggc gtgccggctc gcataatgca
     1321 gcaacgactc gcgccgcttg gccgggcaag cggcgttcag gcagcggtac gctacttccc
     1381 cttccacgcg gtggatctta ctgccgcatt ccgggcaatt cttcggcatg cggaacggcc
     1441 ggcgatcctt cccttccttc accactttca gaacgtgcgg aatcacttcc ccggcgcgct
     1501 ccaccagcac ggtgtcgcca atctgcacgc ccagccgctc gatttcgtcc atgttgtgca
     1561 acgtcgagcg gctcaccgtc acgccgccaa tctgcaccgg ctgcagcagc gtccacggcg
     1621 tcaagacacc cgttcttcct acgttcacct gaatatcgtt cactaccgtc gtttcttgcc
     1681 gcgccggata tttgtaagcg atagcccagc gcggtgattt cgacgtgaat cccagctcgt
     1741 tctgaatcgc cacggaattt acttttacca ccacgccgtc aatttcatac ggcaattttt
     1801 cccgcttcga atcccacgct ccgcaatatt tgatgacttc gtcgatgccg tcgcatagct
     1861 tccagtcgga agatgcgcga aatcgtaaaa ggttcaacgc ttcgagcgat tccgaatgct
     1921 tgggaaacgc aaccctcccg tcgcgcagca ggtaatacgc aaaaaaatcc agtttgcgct
     1981 gtgcggtaac gctggaatcc agtgttcgca cggccccggc cgccgcgttg cgcgcattgg
     2041 caaaaatctt gccgccgctc tgctcctgtt gacggttcat ggcttcaaag gctttgcggg
     2101 tcataatgat ttcgccacgc acttcaaagg aacccaccat tttggccttc ttcagagtct
     2161 ctagcgcgat gcgcagcggg atagaccgga tggtccgcac attcggcgta acgtcttcgc
     2221 cggtcgtccc atcgccgcgt gtgacaccgc gcgccagcac tccgtcttca taaattagcg
     2281 aaatactcag cccgtcaaac ttgtgttcgg caatgtattc gatctcttcc cggccgacgc
     2341 cttcgcgtac tcgccggtca aaatcgcgca gcgcgtcgaa cgaaaaagca ttgtccaagc
     2401 tgagcatcgg ccgcgtatgc tttacggttt ggaaccccgc gcgcggtggc gccccgacgc
     2461 gcgccgtcgg cgaatccggc gtgaccagct gtggatgcgc cgtttc
//
LOCUS       DASTPM010000190         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_352731, whole genome shotgun sequence.
ACCESSION   DASTPM010000190 DASTPM010000000
VERSION     DASTPM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352731"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VFR42_03345"
     CDS             complement(<1..1631)
                     /locus_tag="VFR42_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU0368218.1"
                     /translation="MAAKYIVGIDLGTTNSALAWCDSTAAEESLIEVCSIPQLVNPNE
                     VAERSLLPSFLYIPGEFDFPRGSLALPWEAEPKLVIGELARKRGAESPNRLVASAKSW
                     LSYAAVNRTAPILPWQAPEEVPKLSPLEASSQFLQYLRTVWDSGEAGGQRRLALAEQE
                     VLLTVPASFDEEARELTRRAAEQGGYQQVTLLEEPQAAFYAWLESQGDAWRRRIKVGD
                     LVLVCDVGGGTTDFSLITVSEENGELTLKRVAVGDHILLGGDNMDLALARALQGRLKA
                     SGHLIDTWQLHGLWHQCRLAKEKLFESRKTQKHPVTLLGKGTKLIGGTIKTELTREDL
                     DQVLVEGFFPKVTSDELPARQRRVGFQELGLPYAADPAITKHLARFLSEQVRNSSEAA
                     GIRRGRSGLACPTHILFNGGVMKAAVLRERVVEVLNNWLRQEGLDALRAEQILEAPDL
                     EHAVARGAAYYGKARRGRGVRIRSGASRTYYIGIESAMPAVPGMEAPLKALCVVPFGM
                     EEGTEATIPDREFGLVVGEPAEFRFLSSSVRKQDQA"
     gene            complement(1682..2248)
                     /locus_tag="VFR42_03350"
     CDS             complement(1682..2248)
                     /locus_tag="VFR42_03350"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HEU0368219.1"
                     /translation="MIGFWERVAFAFRCFLSVLLHGDIPNDIAQKLAKPAAPVPQASS
                     PAAPWVSPLREVERPPLETFDRAAQMLTLLQRDGRLIDFLTEDITLYPDEQLGAAVRT
                     IHESCRQVLERYLKLEPILNSEEEQPVTVQAGFDPAAIKLIGNVAGKPPVRGVLRHKG
                     WRVKEMNLPPLPQGAGRMVLAPAEVELT"
BASE COUNT          466 a          780 c          692 g          474 t
ORIGIN      
        1 gcctggtcct gcttgcggac gctggaactc aggaaacgaa attcggctgg ttcaccaacc
       61 acgaggccaa actctctgtc gggaatcgtg gcctcggtgc cctcctccat gccaaaggga
      121 acgacgcaca gcgccttgag cggagcttcc atgccgggca ccgcaggcat ggcgctttca
      181 attccgatgt agtaggtccg cgaagcgcca ctgcggatac gcacgccgcg accacgacga
      241 gccttgccgt agtaggccgc tccgcgcgca acggcgtgct ccaagtcggg ggcttcgagg
      301 atctgctctg cccggagcgc gtccagccct tcttgtcgca accagttatt cagcacctcg
      361 accacccgtt cgcgcagcac cgccgccttc atcacgccgc cgttgaacag gatgtgcgtg
      421 ggacatgcca gtccgctgcg gccgcgccgg atgcccgccg cctcggaact gttgcgaact
      481 tgctccgaca gaaaccgcgc gaggtgcttg gtgatggctg gatcggccgc gtacggcagg
      541 cccaactcct gaaacccgac gcggcgttgt cgcgcgggta attcgtcgct tgttactttg
      601 gggaagaatc cttcgaccag cacctggtca aggtcttcgc gcgtcagttc cgtctttatg
      661 gtgccgccga tgagcttcgt ccccttgcct agcaacgtaa ccgggtgttt ctgcgtcttg
      721 cgcgattcga aaagcttttc cttggcgagg cggcactgat gccataggcc gtgcaactgc
      781 caggtatcaa taagatggcc cgaggccttc aggcgccctt gcagagcgcg cgccagcgcc
      841 agatccatgt tgtcaccgcc cagcagaatg tgatcgccga ccgcgacgcg tttcagcgtc
      901 agttcgccgt tctcctcgga aaccgtaatc aggctaaaat cggtcgtgcc gccgcccacg
      961 tcacacacca gaacaaggtc gccaaccttg attcgcctcc gccaggcatc accttggctt
     1021 tccagccatg cgtaaaaggc tgcctggggc tcctccagca aggtgacctg ctgatagccg
     1081 ccctgttcgg cggcgcgccg ggtcaactcg cgtgcttctt cgtcgaacga agccggtacg
     1141 gtcagcagca cctcctgttc cgcgagagcg agtctccgtt gcccccctgc ctcgccgctg
     1201 tcccacacgg tgcgcaagta ctgcaagaac tgcgaggacg cttctagcgg cgatagcttg
     1261 ggcacctcct cgggcgcttg ccacggcaag atcggcgcag tgcggttcac cgcagcgtac
     1321 gaaagccagg attttgcgga ggcgactagg cggttgggac tctccgcacc gcgtttgcgc
     1381 gccagttcgc cgatgacgag cttcggctcc gcctcccatg gcaaggcgag gctgccccta
     1441 ggaaaatcga attcgcctgg gatgtagaga aacgacggca gcaagctgcg ttccgccact
     1501 tcgttcggat tcacaagctg cggaatgctg cagacctcga tgaggctttc ttcggcagca
     1561 gtgctgtcgc accacgccag cgccgaattt gtggtcccca ggtcgatgcc aacgatgtat
     1621 tttgccgcca atgaaactct cctcttttat acccacacct gttgcgcaga tgtggatcac
     1681 gttacgtaag ctcgacctca gccggtgcaa gcaccatgcg gccggcaccc tgcggcaacg
     1741 gcggcagatt catttccttc acgcgccaac ctttgtggcg taacacaccg cgaactggcg
     1801 gctttcccgc cacgttgccg atcaatttga tggctgccgg gtcaaaaccc gcttgcaccg
     1861 tcaccggttg ctcctcttcg gagttcagaa tcggctccag cttcaggtaa cgctcgagca
     1921 cctgccggca actttcgtgg atggtgcgga cagcggctcc gagctgttcg tcgggataga
     1981 gggtgatgtc ctccgtcagg aaatcgatga gccgcccatc gcgttgcagt aacgtaagca
     2041 tctgcgccgc gcgatcgaac gtttccaagg gcggccgttc aacttccctc aaaggcgaaa
     2101 cccaaggtgc cgcaggggat gacgcctgcg gcaccggagc agccggcttc gccagcttct
     2161 gcgcgatgtc gttgggaatg tcaccgtgca acaggactga gaggaagcac cgaaacgcaa
     2221 aagctactcg ttcccagaat ccgatcatag gtactccgga tgatccaaac cactgcatta
     2281 gaataattca cggccacctg cacgcgtcga gttggctcag ataatcggcg cgatttgcat
     2341 gctcgatgca aaattcaggc ccagacgtag gagcaagcga cacgcacaac cattatcagc
     2401 tatcggcatg ac
//
LOCUS       DASTPM010000191         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_929197, whole genome shotgun sequence.
ACCESSION   DASTPM010000191 DASTPM010000000
VERSION     DASTPM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929197"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFR42_03355"
     CDS             complement(<1..811)
                     /locus_tag="VFR42_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0368220.1"
                     /translation="MTSEHTPAGLIGASIRRVEDPVLVAGKGCYVDDIQLPGMLYLAF
                     LRTTYPHAKIISITTNAAKAMAGVLTVVIGEDVKQLNIPVAPMVPDQKIPPHPLLARG
                     AVHEAGVAVAAVVAHSRALAQDAAGAIEVEYEALPSVIDAEKALEPGAPLAREELDSN
                     ICYVATKKGGDVDKAFAQAEHICRMHIASPRQVALAIEPRGIVVRPEPTGDLTVWLST
                     QAPHRVRGDLATALGFPEQKIRIIAPDVGGGFGSKGPLYREYVLACHLALKL"
     gene            complement(905..1774)
                     /locus_tag="VFR42_03360"
     CDS             complement(905..1774)
                     /locus_tag="VFR42_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0368221.1"
                     /translation="MFPASFGYVAAHSVEEALQLLTKHGEDAKLLAGGHSLIPAMKLR
                     LASPRTLIDLGTVAGLRGVRMDGNTLAIGALTVHTDVASAELVQKHLPGLADAASVIG
                     DLQVRNRGTIGGSIAHADPGADFPVMLTALNASFVLQSTSGSRTVAADDFFIDFYTTA
                     MNANEVLTEIRVPLPTSGSGTAYVKLPHPASCYVVVSAGVLITRQASGSCSLARVALG
                     GLGSGPIRAVVTETELQGKLLAPEVIAAAAAKAAEETDPEGDTYASAEYKRHVATVYA
                     RRAIEAAVRRAIG"
     gene            complement(1761..2267)
                     /locus_tag="VFR42_03365"
     CDS             complement(1761..2267)
                     /locus_tag="VFR42_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0368222.1"
                     /translation="MAAEIEFAAVPIRVTVNGVQHEAVVEPRTLLVYFLREHLSLTGV
                     HVGCDTSQCGACTIQLNGRAVKSCTILAAQANGAEITTVEGLAREGVLHPVQEGFREK
                     HGLQCGYCTPGMIMTAVSLLETNPNPTEEEIRHALEGNLCRCTGYINIVESIRWAAEK
                     MGGKNVSR"
     gene            2541..>3112
                     /locus_tag="VFR42_03370"
     CDS             2541..>3112
                     /locus_tag="VFR42_03370"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0368223.1"
                     /translation="MVCEVTVLMIAFAAVGPAPTRANPYLGKPGEPPATVRAATCALS
                     GGFIHLYAALDNGLFEKYGIKVEFVSVRGSVVSLAALASDEIQFLYCAADATIPGVAA
                     GSDAKLIGAPLVGLPWVLLTRKDIKRPEDLRGKSIAVTRAGDLVYRLARALIRKFNFT
                     EDEVRIRPVGGTGQVEPLNAVFAGLAEACLV"
BASE COUNT          606 a          986 c          866 g          654 t
ORIGIN      
        1 ccagcttgag cgccagatga caggccagca catactcgcg atacagcggc cccttgctgc
       61 cgaacccgcc gccgacgtca ggtgcgataa tccgaatctt ttgttcggga aatccgaggg
      121 ctgtggccag gtcgccacgc actcggtgcg gcgcctgagt agaaagccat acagtgaggt
      181 cacccgttgg ttccggcctg acaacgatcc ctctgggctc gatcgccagc gcgacctggc
      241 gcgggctggc gatatgcatg cggcaaatat gctctgcttg ggcaaaggct ttgtccacgt
      301 cgccgccttt ctttgtcgcg acgtaacaga tgttgctgtc cagctcttcg cgcgccagcg
      361 gcgctccggg ctcgagcgcc ttctccgcgt cgattacaga gggcagcgct tcgtattcta
      421 cttcgatggc gccggcagcg tcctgcgcga gggcgcggct gtgcgctact acggcggcga
      481 ctgcaacgcc ggcttcatgc acggcaccgc gggccaacaa tgggtgcggc ggtatcttct
      541 gatccggcac catcggtgcc accggtatgt tgagctgttt aacatcctcg ccgatgacaa
      601 cggttaggac gccggccatt gccttggccg cgttcgtggt aatggaaatt atttttgcgt
      661 gcggatacgt cgtacgcaga aaagctagat acaacatccc tggcagctgg atgtcgtcca
      721 catagcatcc tttaccggca accagaaccg gatcttcgac ccgccgaatg gatgcgccga
      781 taagtccagc cggagtatgc tcgcttgtca tgggataacc tcttcttgag aaaaaaatcg
      841 cgttcgcgtc catgattcgt gcccgcgatg aaggagagca cgatcacgca tcacgagcac
      901 gaccttaacc tatggcccgc cgcactgccg cttcgatcgc ccgccgcgca tagacggtcg
      961 ctacgtggcg tttgtattct gcgctcgcgt aagtgtctcc ctccgggtcc gtctcttctg
     1021 ccgccttcgc tgcggccgcg gcgatgactt ccggcgccag cagttttcct tgcagctccg
     1081 tctccgtaac cacggccctt atcggaccgc tgcccaatcc gccgagagcg acccgggcca
     1141 aagaacatga gccggatgcc tgccgggtga ttagaacgcc ggcgctcacg accacgtagc
     1201 aggacgccgg atgcggtagt ttgacatagg ctgtgccact accggacgtc ggcaacggca
     1261 cacggatttc cgtcagcact tcattcgcat tcatggcggt tgtatagaaa tcgataaaga
     1321 aatcatccgc ggccaccgtt cggctgcccg atgtcgattg caacacaaac gacgcgttca
     1381 gcgcggtgag catcacggga aaatccgctc ccggatcggc gtgggcgata cttccgccga
     1441 tagtgccgcg attgcgcacc tgcagatcgc cgatcaccga tgccgcgtcc gccaaacccg
     1501 gcaagtgctt ctgcaccaac tccgccgaag cgacgtcggt gtgcaccgtg agggcgccga
     1561 tcgccagcgt attgccatcc atgcgcacgc cgcgcaggcc ggccacggtg ccgaggtcga
     1621 tcaaagtgcg cggcgacgcc agcctgagct tcatcgctgg gatcaagctg tgcccaccgg
     1681 ccagcaactt ggcgtcttcg ccatgcttgg tcaataattg caacgcctcc tccaccgaat
     1741 gcgccgcgac gtagccaaag ctagcgggaa acatttttgc ctcccatctt ttccgcggcc
     1801 cagcggatcg attcgacgat attgatgtag cccgtgcagc ggcacaggtt gccttctaac
     1861 gcgtggcgga tttcttcttc cgtcggattc gggttcgttt ctaacaagct cactgcggtc
     1921 atgatcatgc ccggcgtgca gtaaccacac tgcaagccat gtttttcgcg aaacccttcc
     1981 tgcaccggat gcagcacgcc ttcgcgcgca agaccttcga ctgtggtgat ttccgccccg
     2041 tttgcctggg ccgccaggat cgtgcacgat ttgacggctc ggccgttcaa ttgaatcgtg
     2101 caagcgccgc attggctggt atcgcagccg acgtgcacgc ccgtcagact taaatgctcc
     2161 cgcaagaaat aaaccagcag cgtccttggc tccacaacag cttcatgctg cacaccgttg
     2221 acagtgaccc ttataggaac agcggcaaac tcgatctcag cagccatgct cacctggcct
     2281 ccattcgtac ttcacaacat tcatcgctga aactgtttct tccaaatcgc agacactgat
     2341 cgaagtgctg caaaaaaaat cggtttcgac tgtcatacac cgacttcgtc ccggtctcaa
     2401 tacctcgtgg agaaatcgcg gcgattttca gcggtaaaat cagctgagcc cctctttttc
     2461 aacacttgac atcgcgcttg acaagtacaa tgcgtttcca ctatgaccgg cccatgtcgc
     2521 gccgaggccg tattttccga atggtgtgcg aggtgaccgt tttaatgata gcattcgccg
     2581 ccgtcggccc cgcgcccacc cgagccaacc cttacctcgg caagcccgga gagcccccgg
     2641 caacggtgcg cgcagctacc tgtgccctga gcggcggatt cattcatctc tacgcggctc
     2701 tcgataatgg tctgttcgaa aaatacggga tcaaagttga gtttgtctcg gttcgcggca
     2761 gcgttgtttc gctggcggcg ctggcttccg acgaaatcca atttctctat tgcgccgccg
     2821 atgcgaccat acccggtgtg gcggcaggca gcgacgccaa gctgatcggc gcaccgcttg
     2881 tcggtcttcc ctgggtcctg ctcacccgca aagatatcaa acggccggaa gatttgagag
     2941 gcaagagcat tgccgtcacc cgcgcgggcg atctcgtcta tcggctcgcc agggcgctga
     3001 tcagaaaatt caacttcaca gaagatgagg tgagaatccg gccggtcggc ggcaccggcc
     3061 aggttgagcc tttgaacgca gtctttgccg ggctggctga agcttgcctg gt
//
LOCUS       DASTPM010000192         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1159921, whole genome shotgun sequence.
ACCESSION   DASTPM010000192 DASTPM010000000
VERSION     DASTPM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159921"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /locus_tag="VFR42_03375"
     CDS             complement(<1..1798)
                     /locus_tag="VFR42_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0368224.1"
                     /translation="MNRKKSLGSRESSTRHSSSPSWETLGDFTTTLRVLPISGLAMVV
                     GAVCAFVALALLRLIGLFTNLFYYGRWSCAMVSPVGNHLGWFSVLIPVAGALIIGVMA
                     RYGSERIRGHGIPEAIEAILMNGSRVEPKVALLKPIASAISIGSGGPFGAEGPIIMTG
                     GAFGSMIAQLFHLTAAERKTLLVAGAAGGMSATFASPVAAVLLAVELLLFEWKPRSLI
                     PVALASAVAAVLRRYILGFGPLFPVPQHPLFIGPKALLACVLVGLLAGALSALLTLSV
                     YAAEDFFQRIPIHWMWWPAIGGLAIGFGGLIFPQALGAGYDTIGNLLRGSVTTKVILG
                     VLLVKWFIWAVSLGSGTSGGVLAPLLMMGGALGGLEAMFLPNEGAGFWPLVSMGAILG
                     GTMRSPFTSIIFAFELTHDANVFLPLLVGSVVAHAFTVLTLKRSILTEKVARRGYHLS
                     REYSIDPLELLFAREVMRTKIAALSAASMLREVQHSLRSDHRQKQRLLPVVDAEGRLT
                     GVITRGDIREQIELGGESALDRRLSEMAQTSFVAAFPDEPLRIVVHRMAEKGITRLPV
                     IERDSRKLLGLLSLDDLLKARSRHLEEERHREQ"
     gene            complement(1875..2294)
                     /locus_tag="VFR42_03380"
     CDS             complement(1875..2294)
                     /locus_tag="VFR42_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0368225.1"
                     /translation="MKRISPSEYQAMAELRYRIRLFLREGDAAARTAGLEPQQYLMLL
                     AIRGMPDGSAAKIQSLADSLLIRHHSAVELVDRLEKNGYVRRTRGREDRRQVLVSLLP
                     KGQRVLERVVQQRISELRAGGRQLVRAIDAVLSNGRR"
     gene            complement(2366..>2726)
                     /locus_tag="VFR42_03385"
     CDS             complement(2366..>2726)
                     /locus_tag="VFR42_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0368226.1"
                     /translation="TSPEEMAARLAHLIRHPRFGSVLIAETSERRVIGWVHVSVTPLL
                     EVSLRAEVNGLIVAEGQRSSGAGAKLLQATEEWAKSKGCDSMSVRSNVIRDRAHAFYL
                     RNGYEHYKTQKAFRKKL"
BASE COUNT          628 a          785 c          827 g          486 t
ORIGIN      
        1 tctgttcacg atgccgctct tcttccaggt ggcgcgatct ggctttcagt agatcgtcga
       61 gcgagagcag tcccaggagt ttgcgtgaat cgcgttcgat gaccggcaag cgggtaattc
      121 ctttttctgc catgcggtgg acgacaatgc gcaatggttc gtcgggaaac gcagccacga
      181 aagacgtttg cgccatctca gacaaacgcc tgtccaaagc ggactcgccg cccaattcaa
      241 tctgctcccg aatgtcgcca cgcgtgatga cgccagtaag acggccttcc gcatctacca
      301 cgggaagcag acgctgcttc tggcgatgat cggaacgaag cgagtgctgt acctcccgca
      361 acatgctggc cgcggagagc gcagcgattt tggtgcgcat cacttcgcgc gcgaaaagaa
      421 gctcaagcgg atcaatagag tattcgcggc tgaggtggta accgcggcga gcgacctttt
      481 ccgtgaggat ggaacgcttc agtgtcagca cggtgaatgc atgagcgacg acactgccca
      541 ccagcaatgg cagaaataca ttggcgtcgt gcgtcaattc aaatgcaaaa ataatgctgg
      601 tgaacggcga acgcatggtg ccgccaagaa tggcgcccat actgaccagc ggccagaagc
      661 ccgcgccttc gttgggcaga aacatagctt cgaggccgcc gagcgcgccg cccatcatca
      721 acaaaggtgc gaggacgccg cccgacgtgc cggagccgag cgagaccgcc cagatgaacc
      781 atttaaccag cagaaccccg agaatgactt ttgtcgtgac gctgcctcgc agcagattgc
      841 cgatggtgtc gtagccagcg ccgagcgctt gcggaaaaat caggccgccg aaaccaatgg
      901 ccaggccgcc aatagcgggc caccacatcc agtgaatagg aatccgctga aagaaatctt
      961 ccgcggcgta aacagaaagc gtcaagagtg cagaaagcgc gcctgcgaga aggccgacca
     1021 gaacgcaagc caagagcgct tttggcccga tgaacagcgg atgttgaggc acaggaaaga
     1081 gcgggccaaa tcctaggatg tagcgccgta agacagcggc aacggcgctg gccagggcaa
     1141 ctggaatcag gctgcgcggc ttccactcga agaggagaag ttccacggca agaagcacgg
     1201 cggcgacagg cgaagcaaat gttgcggaca ttccgcccgc cgcgcccgcg acgagcagag
     1261 tcttgcgttc cgccgcggtc agatgaaaca actgggcgat catcgaacca aatgcgccgc
     1321 cggtcatgat gatgggcccc tctgcgccga acggtccgcc cgaaccgatg gagatcgccg
     1381 aagcaattgg cttcagcagc gcaactttgg gttcgacgcg gctgccgttc atcaaaattg
     1441 cttcaatcgc ttcgggaata ccgtggccgc gaatccgctc ggagccgtag cgcgccatga
     1501 cgccgatgat caaagcgccg gctaccggaa tgagcacact gaaccagccc aggtggtttc
     1561 caaccggcga aaccatcgcg cagctccagc ggccgtagta gaagaggttg gtgaagagtc
     1621 cgatcagtcg gagcaaagct agtgcgacaa acgcgcaaac cgcgccgacc accatcgcca
     1681 agccggagat gggcagcaca cgcagcgtgg tggtgaagtc accgagggtt tcccaggaag
     1741 ggctggagga atgacgagta gaactttctc tgcttccgag cgattttttc cgattcacga
     1801 actgctttct ccttgtaaac ctgcggcggg agaaacctgc cgctgcacta aggattcagg
     1861 cgttgcgcac gctctcaacg cctaccatta ctgaggaccg cgtcaatagc gcggacgagc
     1921 tggcggccgc cggcgcgcag ttcgctgatg cgttgttgga ccacgcgttc cagaacgcgc
     1981 tggcccttgg gaagaagcga aacgagcacc tggcgacggt cttcgcggcc ccgcgtgcgg
     2041 cggacgtagc catttttttc cagacgatcc acgagctcca ccgcgctgtg atggcggatg
     2101 agcaggctgt ccgcgagcga ctggattttg gcggcggagc catccggcat tccgcgaatg
     2161 gcaaggagca tgaggtactg ctgaggctcc aggccggcgg ttcgcgcagc ggcatcgcct
     2221 tcgcggagaa atagccggat gcggtaacgg agctctgcca tggcctggta ttcggaagga
     2281 cttattcgct tcatagaaat attatatcgt agaacgatat gctttgccac agcggtgcga
     2341 gaatgaaaac cagttcacag aatccttaca actttttacg gaaggccttt tgcgttttgt
     2401 agtgttcgta gccgttgcgc agataaaaag catgcgcacg gtcgcggatg acgttagagc
     2461 ggaccgacat gctgtcgcag cccttggatt tggcccattc ttcggtggct tgcagaagct
     2521 ttgcgccggc gccgctgctg cgttgccctt cagcgacgat caacccattc acttcagcgc
     2581 gcagcgaaac ttcgagcaga ggagtgacgc tgacgtgaac ccagccaata acccgacgct
     2641 cggaagtctc agcaatcagg acgctgccaa aacgtggatg gcggatcagg tgagcaaggc
     2701 gcgcggccat ttcttccggc gacgtt
//
LOCUS       DASTPM010000193         5913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_785277, whole genome shotgun sequence.
ACCESSION   DASTPM010000193 DASTPM010000000
VERSION     DASTPM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785277"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFR42_03390"
     CDS             <1..291
                     /locus_tag="VFR42_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0368227.1"
                     /translation="LAMPGLRFFGITDPKRFEERCSTVSFRLAKGAPTDAATFLGDRG
                     IFTWDGNFYALNLTERLGVEQHGGVLRVGLVHYNTADEVDRLLAALREFASL"
     gene            298..627
                     /locus_tag="VFR42_03395"
     CDS             298..627
                     /locus_tag="VFR42_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368228.1"
                     /translation="MAVPHNPVPTPRSYRLVSFHALRALFWQIISTLLHACGMILMFA
                     TASGHHGFHATHKRQRTLRFEQLFVVLPFFWLIMMGGFATLLTLGIIYCLKAMRGEWA
                     GFPLISR"
     gene            671..2230
                     /gene="gpmI"
                     /locus_tag="VFR42_03400"
     CDS             671..2230
                     /gene="gpmI"
                     /locus_tag="VFR42_03400"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU0368229.1"
                     /translation="MTKRPKPIVLTVLDGWGYRAETKGNAIALARKPAYDHLVQNFAN
                     TLIETSGPAVGLPDGQMGNSEVGHMNIGAGRVVRMDITKIDVLVANKSLGEVPLFQRA
                     MERSRQRQLHFLGLLSDGGVHSHIQHLFALLELAKQQKVENVFVHCFMDGRDTPPHSG
                     RGFVQQLQQRMRELGVGKIATVVGRYYAMDRDNRWERIQLAYNAMVHGEAESHSSDPI
                     AALSASYEAGVTDEFVKPVVITDGDSASAKPVGLIHDDDAIIFYNFRADRARQMTYAL
                     AVPDFDKFADPKRPKNLFYVAMTQYDKNWPWLQSVIKPDKLEHILAQVFADLNYKNLR
                     TAETEKYAHVTYFFNGGVEKPFSGEERILVPSPKVATYDLKPEMSAAGITDTVVKAIE
                     KGEFDAIIMNYANADMVGHSGKLEAAIKAVEAVDAGLAKILQTLKPRGGAWIITADHG
                     NAETMIDPVSGGPHTYHTTNPVPFILVTENDKLKLKSGGALRDIAPTLLGILGQNRPA
                     EMTGTDLRVLS"
     gene            2230..3255
                     /locus_tag="VFR42_03405"
     CDS             2230..3255
                     /locus_tag="VFR42_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0368230.1"
                     /translation="MSQTPDTARRSPAETKLVVCVKHAFSEWCAKPMLADAIRKGWPQ
                     MRVVHLPDYSRIKEELPDTHIFVGASLRAEQFAHAKQLQWIHSTAAGVSQLMYPELRS
                     SSVIVTNPSGIFSVPMAGHTMGLILALARNFPDSVRFQDAAKWAQQDLWDKTQHLTEI
                     NGSVLLIVGYGSIGRELAKRAQVFDMRVWGVTRSGKGDTAHAEKILPAADLHNALPQA
                     DYVVIAAPETAETKHLIGVEQIARMKRGARLINVARGSLLDEAALISALQTGQLSGAA
                     LDVTSEEPLPADSQLWKAPNLFITPHTSGVSDRLWHRQTELLLRLLEEWFAGKELSNR
                     VGLSRGY"
     gene            complement(3298..4392)
                     /locus_tag="VFR42_03410"
     CDS             complement(3298..4392)
                     /locus_tag="VFR42_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0368231.1"
                     /translation="MKRMLANALRGLVFAATLSALGISLALAQAPTGPAPGGQPQQTP
                     PPGQNPPQQQAKPQEGGLSIAVEVPVVTMDVVATTQRGDIITGLKRENFRLFEDGVPQ
                     TITNFGPTDAPITMVVLMEFSARYGGYFSYVAKYWTDALFPQLKKDDWVALVTFDMKP
                     KLEVDFTQNKDEVRQAVYHLYFPGFSESNVFDALLDTTERLRDVKGKKCILLIATGRD
                     TFSKHTYDQTMKALRQSDVPVFIVGMSKAIQNMRDMRYPMASDIDLLQDENEMKTFAK
                     LTGGFAWFPQFTGEIPGIFQDVAAFLRNQYSLSYSPTNRTRDGKFRKVKIDLVAPDGS
                     AFTVTDQKGKKQKYQVYAREGYSAPQGAVD"
     gene            4581..>5913
                     /locus_tag="VFR42_03415"
     CDS             4581..>5913
                     /locus_tag="VFR42_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0368232.1"
                     /translation="MQGARKSVFSARFVAIFAAISATFAIALTAQTALGQVPGIGQPV
                     VMQTNSGPGDTSALAREATTWLAELIKINTTNPPGNEEAAAKYIAGILQKEGIKAELL
                     PLAPGRSALVARLNASAFADPSKALLLVAHMDVVGVERAKWTVDPFGAVPKDGYIYGR
                     GAIDDKGMLAANLAAFVYLKRTNARLNRDIIFLATDDEEGFGNASVKTLIAKYWDKIA
                     AKYSINEGGNVFLKDRKVQYVAVQASEKVSMNVTVTAKGSSGHASIPAKDNAVTHLAA
                     AVEKIGNYTPPVRFTTIVRRYFEGIAPLEDGDIAKWIRSLDTPDRGEHAARVIADAKP
                     LWNAMLRDTISPTMLNAGVRPNVIPSEATATLNVRLLPGNTITTLLSELNKLVNDPQV
                     KLEVEKDAGLAAPDSSLDSDFYQAIVKASAEEFAGAPVLPFQSTWATDSAQL"
BASE COUNT         1273 a         1778 c         1685 g         1177 t
ORIGIN      
        1 ctcgccatgc ctggcctgcg cttcttcggc atcaccgatc ccaaacgctt cgaagagcgc
       61 tgttctaccg tttcgtttcg cctcgccaaa ggcgcgccca ccgacgccgc cacattcctc
      121 ggcgaccgcg gcatcttcac ctgggacgga aatttttatg ccctcaatct caccgaacgc
      181 ctcggcgtcg aacagcacgg cggcgtcctc cgcgtcggcc tcgtccatta caataccgcc
      241 gacgaagtcg accgcctgct cgccgccctc cgcgaattcg cgtccttata aatgatcatg
      301 gccgtcccgc acaacccagt cccaactccg cgaagttacc gattggtcag ttttcacgct
      361 ctgcgggcgc tcttctggca gatcatctct acgctgctcc atgcatgcgg aatgatcctg
      421 atgttcgcta ccgcgtctgg gcatcatggc ttccatgcca cacacaaaag acagcggacg
      481 ctccgtttcg aacagctttt cgtcgtgttg ccgttcttct ggctcatcat gatgggagga
      541 ttcgccacct tactcacgct cggaatcatc tactgcctca aggccatgcg tggcgaatgg
      601 gccggctttc cactcattag ccgctaggca gagcgaatcg tcagaaacaa aaatggtatt
      661 ctgatccgct atgaccaaac gaccgaaacc aatcgtattg accgtgctgg acggctgggg
      721 ctatcgagcg gaaaccaaag gcaacgccat cgctctggcc cgcaaacccg cttacgacca
      781 tctggtccag aattttgcca acacgctgat tgaaacttcc ggccccgccg tcggcctgcc
      841 cgatgggcaa atgggcaaca gcgaagttgg ccacatgaat atcggcgccg gccgggttgt
      901 ccgcatggac atcaccaaga tcgacgtgct ggtggccaac aaatcgctcg gcgaagttcc
      961 gcttttccag cgagcgatgg agcgcagtcg ccagcgccag ttgcatttcc tcggcctgct
     1021 cagcgacggc ggcgtccact cgcatattca gcatctgttc gcactgctcg aacttgccaa
     1081 acagcaaaaa gtcgaaaacg tttttgtcca ctgtttcatg gatggccgcg atactccgcc
     1141 gcacagtggc cgcggctttg tccagcagct acaacaaaga atgcgcgagc tgggcgtcgg
     1201 caaaattgcc actgttgtgg gccggtacta cgcgatggac cgcgacaatc gttgggagcg
     1261 tattcagctt gcttacaacg ccatggttca cggcgaagcc gaatcacact ccagcgatcc
     1321 catcgctgcg ctcagcgcca gctacgaagc cggcgtcacc gatgaattcg tcaagcccgt
     1381 cgtcatcacc gatggcgata gcgcttccgc gaaacccgtc ggcctcattc acgacgacga
     1441 cgcgatcatc ttctacaatt tccgcgccga ccgcgctcgc cagatgacct acgcgctcgc
     1501 agttcctgac tttgacaagt tcgccgatcc caagcgcccc aaaaatcttt tctacgtagc
     1561 catgacgcaa tacgacaaga actggccctg gctgcagtcc gttatcaagc ccgacaaact
     1621 tgaacacatc ctcgcgcaag tcttcgccga ccttaactac aaaaatctgc gaaccgcgga
     1681 aaccgaaaaa tacgcgcacg taacctattt cttcaacggc ggcgtcgaaa agccgtttag
     1741 tggcgaagaa cgcatactcg ttccttcgcc caaagtcgcc acctacgatc tgaaaccgga
     1801 aatgtccgcc gcaggcatca ccgacaccgt ggtaaaagcc atcgaaaaag gcgagttcga
     1861 cgccatcatc atgaattacg ccaacgccga catggttggc cactccggca aactcgaagc
     1921 cgccatcaag gccgtcgaag ccgtggacgc cggcctcgcc aagatcctcc agactttgaa
     1981 gccgcgtggc ggcgcctgga tcatcaccgc cgatcacggc aacgccgaaa ccatgatcga
     2041 tccggtcagc ggcggcccgc acacctacca caccaccaat cccgtcccgt tcatcctggt
     2101 caccgaaaac gacaagctca aactgaaatc cggcggtgcc ctccgcgaca tcgcccccac
     2161 tctgctcggc atcctcggcc agaaccgccc cgccgagatg accggcaccg acctgcgcgt
     2221 tctttcgtaa tgtcccaaac gccagacacc gcacgacgca gcccagcgga aaccaagctg
     2281 gtggtttgcg tgaagcatgc gttcagcgaa tggtgcgcca agcccatgct ggccgacgcg
     2341 attcgcaaag gttggccgca gatgcgcgtc gtccatcttc ccgactattc gcgcatcaag
     2401 gaagaactgc ccgacacgca catctttgtc ggcgcatcat tgcgtgctga acagttcgcg
     2461 catgcaaagc aactacagtg gattcactcc acggcagcgg gcgtttcgca actgatgtat
     2521 ccggagttgc gcagctccag cgtgattgtg acgaatccaa gcggtatctt ttccgtgccg
     2581 atggccgggc acaccatggg gttgattctc gcgctggcgc gcaacttccc ggactcggtg
     2641 cgctttcaag atgccgcgaa atgggcgcaa caggatttgt gggacaagac gcagcacctc
     2701 acggaaatta acggcagcgt cctgctcatc gtcggctacg gttccatcgg ccgcgagcta
     2761 gcgaaacgcg cccaagtatt cgacatgcgg gtgtggggtg tgacgcgctc cggcaaaggc
     2821 gacacagcgc atgcagagaa aattctgccc gctgcggatc tgcacaacgc gctgccccaa
     2881 gccgattacg tcgtgatcgc cgcgcccgag acagcagaaa caaaacatct gatcggcgtg
     2941 gagcagatcg cgcggatgaa gcgcggtgct cgcttgatta atgtcgcccg cgggtcactg
     3001 ctggatgaag cagcactgat cagtgcgctg caaaccggcc aactgagcgg tgcggcactc
     3061 gacgtcactt ccgaagaacc attgcctgcg gattcgcagc tgtggaaggc gccgaatctt
     3121 tttattacgc cgcataccag cggagtgagc gatcgcttgt ggcatcgcca aacagagttg
     3181 ctgctgcgcc tgctcgagga atggttcgcc ggaaaagaat tgtcaaaccg cgtgggtttg
     3241 tctcgcggct actaacctct cctggtgcaa ccggtcggga taaacccgcg gctacattca
     3301 gtcgacggca ccttggggcg cgctgtagcc ttcgcgggcg tacacctggt atttctgttt
     3361 tttacccttc tggtcagtga ccgtaaacgc cgaaccgtcc ggcgcgacca aatcaatttt
     3421 gactttgcgg aattttccgt cgcgcgtgcg gttggtcggg ctgtagctca ggctgtactg
     3481 gttgcgcaaa aacgccgcta cgtcctgaaa aattccggga atttccccgg tgaattgggg
     3541 aaaccaggca aagccgccgg tcagtttggc gaaggtcttc atttcgtttt cgtcctgcag
     3601 cagatcaatg tcgctggcca tgggataacg catgtcgcgc atgttctgaa tggctttgct
     3661 catgccgacg atgaacaccg gaacatccga ttgccgcagc gctttcatgg tctggtcgta
     3721 ggtgtgtttt gaaaaagtgt cgcggccagt ggcgatcagc aagatgcact ttttgccttt
     3781 tacatctctc aggcgctcgg tggtgtcgag cagcgcgtca aaaacattcg attcgctgaa
     3841 gccggggaag tacaagtgat agacggcctg gcggacttca tctttatttt gcgtgaagtc
     3901 tacctcgagc ttcggtttca tgtcgaacgt gaccagagct acccagtcgt cttttttcag
     3961 ctgagggaag agtgcgtcgg tccaatactt cgcgacgtaa gaaaagtagc cgccatagcg
     4021 cgcgctgaat tccatgagca cgaccatggt gatcggcgcg tccgtcgggc caaagttggt
     4081 gatggtttgc ggaacgccgt cttcaaagag ccggaaattc tcgcgtttca gacccgtgat
     4141 gatgtcgccc cgctgggttg tggcgaccac gtccatggtg acgacgggaa cttcgacggc
     4201 gatcgataag ccgccttcct gcggctttgc ctgttgctga gggggattct gtcccggagg
     4261 tggagtctgt tgcggctggc ctccgggcgc cgggccggtc ggagcctgcg caagggccag
     4321 agaaattccc agtgctgaaa gcgttgcggc aaaaaccaga ccacggagcg cgttcgcgag
     4381 cattcgtttc atccttgtct cccaaaaccg gggcggctcg ccagaggccg accgtggaac
     4441 ttaggaacgc gcagcgattg cgtgagccag gcgcttccca gtggcatgtt agtatgaagc
     4501 agatttgaag caaaacgttt gctaagcggc cgctcgctgc agaaaacgtg gcccgaccgg
     4561 gagaagataa ggtgcaaagc atgcagggcg caagaaaaag tgtgtttagc gcgcgttttg
     4621 tggcgatttt tgcggcgatt tccgctacgt tcgcgatcgc cttgaccgcc cagacagctt
     4681 tgggacaggt gccagggatc gggcagccgg ttgtaatgca gactaactcc ggcccgggcg
     4741 acacttccgc gttggcgcgc gaagctacga cgtggctcgc cgaactgatc aagatcaaca
     4801 ctaccaatcc gccggggaat gaagaagctg ccgccaaata tattgcagga attttgcaaa
     4861 aagaggggat taaggcggag ttgctgccgc tcgctccggg gcgcagcgcg ctggtcgcgc
     4921 ggctgaacgc ttcggcgttt gccgatccgt cgaaagcgct gctactggtg gcgcacatgg
     4981 atgtcgtggg tgtggagcgc gcgaagtgga ccgtggatcc cttcggcgcg gtgcccaagg
     5041 acggctacat ctacggccgc ggagcgatcg acgacaaggg aatgctggcc gcgaacctgg
     5101 cggcgttcgt ttacttgaaa cgaacgaacg cgcgcctgaa ccgcgacata atttttctgg
     5161 cgaccgacga cgaagaaggc ttcggcaatg ccagcgtcaa gacgctgatt gctaaatact
     5221 gggacaaaat cgccgcgaag tattcgatca acgaaggcgg caacgtcttc ctgaaagacc
     5281 gcaaagtgca atacgtggcg gtgcaagcca gcgaaaaagt ttccatgaat gtgacggtga
     5341 ccgcgaaagg gtcttccggc cacgcttcga ttccggcgaa ggacaacgcg gtgacgcatc
     5401 tcgcggcggc ggtggaaaaa atcggaaatt acactccgcc ggtccgcttc acgacgatcg
     5461 tgcggcgtta ctttgaaggg attgcaccgc tagaagacgg tgatatcgcg aagtggattc
     5521 gttcgttgga cacgccggat cgcggcgaac atgcggcgcg cgtgatcgca gacgccaagc
     5581 cgctgtggaa cgccatgctg cgcgacacca tttcgccgac catgctgaat gccggcgtac
     5641 ggccaaatgt gattccatcg gaagcgacgg caaccctcaa cgtgcgtctg ctgccgggca
     5701 acacgattac cacgctgctc tccgagctga acaagctggt gaacgatccg caggtgaagc
     5761 tggaagtaga gaaggatgcc ggcctggcgg cgccggactc gtcgctggac tcggatttct
     5821 atcaagcgat cgtgaaggcg tcggcggaag aatttgctgg agcgccggtg ctgccgttcc
     5881 agtccacctg ggcgacggac tcggcgcaac tgc
//
LOCUS       DASTPM010000194         3933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_179725, whole genome shotgun sequence.
ACCESSION   DASTPM010000194 DASTPM010000000
VERSION     DASTPM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179725"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..1359
                     /locus_tag="VFR42_03420"
     CDS             364..1359
                     /locus_tag="VFR42_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU0368233.1"
                     /translation="MKRIAWILGILSLGGAFGPASGQTPATAPAGGAQKIILDTDIGD
                     DIDDAFALALALRSPEVEVIGITTAWGDTQLRARLANRLVKQGGRGSIPVLAGVPTKG
                     KTNFTQADWARDGEAAPARGDGVAFLLDQAQKAPGEVMLVAIGPLTNIGAAIDRDAEA
                     FRKFKRVVLMGGSIRRGYGDLGYGPDRGPEPEYNIYSDVKAAQKLFASGVPIFMMPLD
                     STQLLLDESKRTLLFSTGSPLTNALAALYFQWVRGTRTATATLFDAMAVAYAVDPALC
                     PVEPFHITVEENGLTKPTEGTPNASACLASDSEKFFHFLLPRLMTSPVRDASSKN"
     gene            complement(1398..1847)
                     /locus_tag="VFR42_03425"
     CDS             complement(1398..1847)
                     /locus_tag="VFR42_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368234.1"
                     /translation="MANPSTAIVSAGTICGIMDITAALVVYGTMGAKPLRLLQGIAGG
                     VLGPRTYTGGIATALLGLALHFVIAFGAATVFFIASRGIGFLLDHAVVSGALYGIAVY
                     FFMQRVVIPLSRANRNPFSLKFMIIGIIIHIFCVGLPIALSVRKHSR"
     gene            complement(1928..2767)
                     /locus_tag="VFR42_03430"
     CDS             complement(1928..2767)
                     /locus_tag="VFR42_03430"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0368235.1"
                     /translation="MTTVRTEALRQSDRITLRLPLEASWVDGSGLIRRLPAQTLLVSR
                     NGGVLRLQDAFTAGQELTLKRPLEGDAVKTVRARILAEIDREPEGFLYAIHILESRAD
                     FWDIEFPPPHKAEEALARLLMECSFCQRREVVYLNELELKSFEARKCVARICKICDAP
                     SIWIEAQSAISESGTVVSRSPGEERVLPRRNRTRVKARVLACIRRRGFQEEVAVCEDL
                     SKGGIAFRSRNQYPEGSRAEVAVPFTPGSSAIFVPIRIVFSQAIPSAGLFRHGAAYLK
                     PPG"
     gene            2996..3373
                     /locus_tag="VFR42_03435"
     CDS             2996..3373
                     /locus_tag="VFR42_03435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0368236.1"
                     /translation="MKNVISMDEHLNKSGSRSLLVRDRRYAIRHPFAADATFLDLETG
                     AAADGVTSDLSMGGCFVCTSKPLAAGCRMRLTLRRKEETVQALAVVRIFKPRVGMGLE
                     FLDVEERHQPTLERWLEQLRRDR"
     gene            complement(3410..>3933)
                     /locus_tag="VFR42_03440"
     CDS             complement(3410..>3933)
                     /locus_tag="VFR42_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368237.1"
                     /translation="FLAKLGVTGCRWFGGGVGSYFEVCRKALVGDVPPSISPDQPIVV
                     TLKILFSYPGLSTEDQGHGGRAQLITTSFREYERRIREQFAEMFSGVGFDSRRDIAGI
                     ILNRWGHAYLSPQPGFFFGTAGKPAPREVLRSAPFGRIAFANTDLAGAMDHRYSILEA
                     RRAVSQLCDSVIS"
BASE COUNT          897 a         1019 c         1279 g          738 t
ORIGIN      
        1 accatggctg cgccgagaaa tggggtttca tcttcgaggc gcagacaaat cttaagggtc
       61 gcttggccga ccggcgggtt tgaatcgagc aacgcgttgg taggagaggt ttgcgcagag
      121 aggctagcgg ccgccagtag agaaactgca gcaactaagg gagacagacg cgattgacgc
      181 caacccatgc ggacctgcgc agaaacactg gctaaatgaa acagcggagt caggaaagtg
      241 tcaagtcaac tttgtgtcag gcgggggagg cgacgggcta gcgaggtcag gctcgccgat
      301 tccctggcct aagcgtctca ggtccgaaat gttaagctgg gcggcctgtg atgggagccg
      361 agcatgaaac ggattgcgtg gatcctgggg atcttaagtc tgggcggggc attcgggcca
      421 gcctccggcc aaacccctgc gaccgctccc gccggcggcg cgcagaagat tatcttggat
      481 acggacattg gtgacgacat tgacgacgcc tttgcgctgg cactcgcgct gcgcagcccg
      541 gaagttgaag tgatcgggat taccacggcc tggggagaca cgcagctgcg ggctcgcttg
      601 gcgaatcgtt tggtgaaaca gggcgggcgc ggctcgatac ctgtgctggc cggagttccg
      661 acaaaaggca aaacgaattt cacacaggca gattgggcgc gggatggaga ggcagcgccc
      721 gctcgagggg atggcgtggc gttcctgttg gatcaagcgc aaaaggcgcc gggagaagtt
      781 atgctggttg cgatcggccc gctcacgaac atcggcgcgg caattgaccg tgacgcggag
      841 gccttccgca agttcaagcg cgtcgtgctg atgggcggtt cgatccggcg gggatatggg
      901 gacctcggct acgggccgga tcgcgggcca gagccggagt acaacatcta cagcgacgtg
      961 aaggcagctc aaaaattgtt cgcgagcggc gtgccgatct tcatgatgcc gctggactcg
     1021 acgcagcttt tgctggatga gagcaagcgc accctgctgt tcagcacggg ttcgccgctg
     1081 accaatgctt tagcggcgct gtacttccag tgggtcagag gaactcggac cgcgacggcg
     1141 acgttgttcg atgcgatggc ggttgcttat gcggtggatc cagcgctgtg tccggtggaa
     1201 ccattccaca ttactgtgga ggagaacggc ctaacaaaac ctacggaggg aacaccgaat
     1261 gcgtcggcct gcctggcttc tgactcggaa aagttctttc acttcctgtt gccacgattg
     1321 atgacctcac ctgtacggga tgcaagctcg aagaattaat ttcatcacaa gagcagactg
     1381 aagatcggtc ctgaatctca gcgtgagtgt ttgcggacgg aaagagcgat cggcaggccc
     1441 acacaaaaga tgtggataat gatgccgatg atcatgaact tgagcgaaaa gggattgcga
     1501 ttggccctgg agagcgggat gacgacgcgt tgcatgaaga aataaacggc gatgccgtac
     1561 aaggccccag aaaccactgc atgatcgagc aagaagccaa tgccgcggct ggctataaag
     1621 aaaactgtcg cggcgccgaa ggcgatgaca aaatgcagcg cgagtccgag taatgcggtt
     1681 gctattccac cggtataagt gcgtggacca agaactccgc cggcaattcc ttgcagaagg
     1741 cgcagaggct tcgcgcccat cgtcccgtaa acaacgagag ctgcagttat atccataatt
     1801 ccgcagatgg tgccagcgga aacgatcgct gtgcttggat tggccatgcg ctgctccaga
     1861 gtcaggtgaa gtggcacgca gattctagat ggattgcaga gaaccgccaa ccttgcggag
     1921 ataactttta tccgggaggc ttgaggtaag cagcgccgtg gcggaaaagg ccagccgacg
     1981 ggatggcttg agagaagaca atgcgaatgg gaacgaaaat ggcgctgctg ccaggggtga
     2041 acggaacggc aacctcggca cggctaccct cgggatactg gttgcgactg cgaaaggcga
     2101 tgccgccttt agacaggtct tcacaaaccg ccacctcttc ctgaaaacca cgacgccgga
     2161 tgcaagccag gacccgcgct ttgacacgag ttcgattacg gcgcggtagg acgcgttctt
     2221 cgcccgggct gcgcgaaacc accgtaccgg attccgaaat tgccgattgc gcttcgatcc
     2281 agatggaagg agcgtcgcag attttgcaga tgcgagcgac gcacttacgc gcttcgaaag
     2341 atttcagttc cagttcgttg aggtacacca cttcacggcg ctggcaaaaa ctgcattcca
     2401 ttaacaagcg ggcgagggct tcttcagctt tgtgaggagg aggaaactcg atatcccaga
     2461 agtccgcgcg ggactcgagg atgtggattg catacaagaa gccttcgggt tcgcggtcga
     2521 tctcggcgag tatgcgggca cgaacagttt tgacagcgtc gccttccaat ggcctcttca
     2581 gagtaagttc ctggccggca gtgaaagcgt cctgcaagcg aaggacgccg ccattgcggc
     2641 taaccagaag cgtttgggcg ggcaaacggc gaatcaatcc ggagccatcc acccaggatg
     2701 cctcgagggg caggcgaagg gtgatgcgat cgctttgacg cagggcttcg gtgcgaacag
     2761 tggtcatgag aggcaaagac cgcacatgaa gtcagtgtag caattccaag agagacggct
     2821 ggcgcaggaa tggcgaactt tgtcgcatgc actaataacc gcccgcggca ggattggtac
     2881 caaagaactg ttatctctta agcagattct ggcgcgagtg caggcctgga gaaaccgatt
     2941 tcagtcaaac tggtggacac cgcactgcga gaatgacgaa cggaactcag ctgccgtgaa
     3001 gaacgttatt tccatggacg aacatctcaa caagagcgga tcgcgctcgc tgctggtacg
     3061 cgaccggcga tacgcgattc ggcatccatt tgccgcagac gcgacttttc tggatttgga
     3121 gacgggcgcc gctgccgatg gcgtgacgtc ggacctttcg atgggcggtt gttttgtttg
     3181 taccagcaag ccgctggcgg cgggctgccg catgcgtttg acgctgcggc gcaaagaaga
     3241 aacggtgcag gccttggcgg tggtgcggat tttcaagccg agggtcggca tggggttgga
     3301 gtttcttgac gtcgaggagc ggcatcagcc cacactggag cgctggctgg agcagttgcg
     3361 gcgcgatcgc taaatccgta cgtttcttcg cttacattct tccgaccaac taggaaatca
     3421 cggaatcgca gagctgtgag acggctcgcc gcgcctccag aatggagtag cggtgatcca
     3481 tggcgcccgc gagatctgtg ttggcgaatg cgatgcggcc aaaaggcgcg gaacgcaaga
     3541 cttcgcgggg cgcgggtttg ccggcagtgc cgaagaaaaa accgggctga gggctgagat
     3601 aggcgtggcc ccagcgattc agaatgatgc cggcgatgtc gcgcctggag tcaaagccga
     3661 cccctgaaaa catttcggcg aactgttcgc ggattcggcg ttcgtattca cggaacgaag
     3721 tggtgatgag ctgtgcgcgg ccgccatggc cctgatcttc ggtggaaagg cctgggtacg
     3781 agaaaaggat tttcagagtg acgacgatcg gctggtcggg gctgatggag ggtgggacat
     3841 ctccgacgag agccttgcgg cacacttcga aatagctgcc gacgccgccg ccaaaccaac
     3901 ggcagcctgt gacgccgagt ttcgccagaa aac
//
LOCUS       DASTPM010000195         3653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_410706, whole genome shotgun sequence.
ACCESSION   DASTPM010000195 DASTPM010000000
VERSION     DASTPM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410706"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..869)
                     /gene="lepB"
                     /locus_tag="VFR42_03445"
     CDS             complement(117..869)
                     /gene="lepB"
                     /locus_tag="VFR42_03445"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0368238.1"
                     /translation="MANSSNPGHEGNTDWLTSLQSLLATVVIAVFVITYIVQAFQIPS
                     VSMENTLLIGDYLLVDKLCYGGSGSYLVPYRPIRRGDIIVFHYPVNPSQHFVKRVIGV
                     PGDRIHLVNKRVFVNGTPLPEPYVRYFKPERNFFRDDFPRLDVLDPGINADWWVQMPK
                     LVEDGQLIVPEGHYFAMGDNRDDSSDSRYWGFVPRENIVGRPLLIYWSIRNLDNDLPI
                     PASVGDRLYHFAYAVTHFFQITRWDRTFRVVS"
     gene            complement(870..1667)
                     /gene="rnc"
                     /locus_tag="VFR42_03450"
     CDS             complement(870..1667)
                     /gene="rnc"
                     /locus_tag="VFR42_03450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU0368239.1"
                     /translation="MKVKEIAALEGLLGYHFERPELLDQALTHSSQARELEALQPLKT
                     GVRPGDNEQLEFLGDAVLALVTGEELFRRFPTFREGELSKLRAHLVSERHLIQVAQGL
                     NLGGYLRLGRGEEKSGGRSKTALLVDAFEAVLAALYLDGGLETARRFILQTVVAPELE
                     RLNPASGGVPVMDFKSALQENLQATGRQASYVLVKEAGPEHSKTFTVEVRLSSPGMSK
                     VEFVGRAEGTTKKKAEQDAARQVLEYLAAFPAVAEEKVGSKSPARPT"
     gene            complement(1735..2619)
                     /locus_tag="VFR42_03455"
     CDS             complement(1735..2619)
                     /locus_tag="VFR42_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368240.1"
                     /translation="MKRVAMIGAVLAVATWGVAQSNSKPDTQKAAQPGTAQTGTQAPA
                     AAQGKRPPQAKTQPEYDAYKTAVALTDAAAAEKAADDFATKFPDSELRVMLYKSAMHT
                     YQKAGNSDKIIEMGRKGLAIDGDDPELLVTVASELAEKTRDTDLDKDQRLDEAMKMAQ
                     KSLQTVDTDISIPPGTPQDQVDGYKGQLRSFAYSIIGTLEFKKDNFAAAETDLRKSID
                     AFPSQPDPVVVLRLAISLDRQNKYPEALKEANHAVELTQETTSAGKLARQERDRLNQL
                     VGGNAAAPKPATPEQPKN"
     gene            complement(2661..>3653)
                     /locus_tag="VFR42_03460"
     CDS             complement(2661..>3653)
                     /locus_tag="VFR42_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368241.1"
                     /translation="TEIYLKLPSAGYLGRGFSVFLEPMGAPSQVNARNYGSDYFVVIS
                     PAGQELKMDQIRHTYLHYLLDPLSLKYPTSMKRLEPLLESVKGAPMEESFKIDTSLLV
                     TECLVRAVEIRMQGMSKTPEALRDDAIEKSAEQGFVLTRYFYDSLAQFEKAPEGIRNA
                     YIDLLANVDLKKEQKRAAETHFASAAVPDVLHFARPKQQHLLVLAEKRLAAGDLKTSQ
                     ELAQQALHDGHEDAGRALFILAQVATANRDMAGATGYFEQALKVAQEPKVIAWSHIYL
                     GRIFDLQERRENALDEYHAALNAGGTLPEVKAAAERGLQQPYEPPASRQQSDKK"
BASE COUNT          693 a         1042 c         1069 g          849 t
ORIGIN      
        1 aaccagcact gcggccagag aggcaaggaa ctctaccgtg gtctcgcttt gtttctctgc
       61 cggaggattt tctttcttag ccattttccg ttgaatccag gatgacattg aaattgtcag
      121 ctgacgacgc gaaatgtacg gtcccagcgg gtgatctgaa agaaatgcgt taccgcgtat
      181 gcgaaatgat agagtctatc acccaccgag gcaggtatcg gaagatcgtt gtcgagatta
      241 cgaatcgacc agtagatcag cagcggccgt cccacgatat tctcgcgcgg cacaaatccc
      301 caataccggc tgtcggaact gtcatcgcga ttgtccccca tcgcgaagta gtgaccttcc
      361 ggcacgataa gttgtccgtc ttcgacgagt ttcggcattt gcacccacca gtccgcgttg
      421 atgccgggat cgaggacatc cagccgggga aagtcgtcac gaaagaagtt tcgttccggc
      481 ttgaaatagc gaacgtacgg ttccggcagc ggcgtgccat tcacgaaaac gcgcttgttc
      541 accaggtgaa tgcggtctcc cggtacgccg atgactcgct ttacgaaatg ctgggaagga
      601 ttgacgggat agtggaacac aatgatgtcg ccacgtcgaa tcggtcggta gggcacaagg
      661 tagcttccac ttccgccata gcagagtttg tcgacgagca ggtagtcgcc gatcagcaat
      721 gtgttttcca tcgagacgga gggaatctgg aaggcctgca cgatgtaagt gatgacgaaa
      781 accgcaatca ctaccgtcgc aagcagcgat tgcagggagg tcagccagtc agtgttgccc
      841 tcatggcccg ggttggacga gttcgccatt tacgttggac gcgcgggaga tttcgatccc
      901 actttttctt ccgcgaccgc cggaaaggcg gcgaggtatt caagaacctg gcgcgccgca
      961 tcttgctccg ctttcttctt ggttgtgccc tccgcccggc caacgaattc gactttgctc
     1021 atcccgggag acgagagtcg gacctccacg gtgaatgtct tgctgtgttc cggtcctgcc
     1081 tcttttacca ggacgtagga ggcctggcgg ccggtggctt gcaggttctc ttgcaaagcg
     1141 gacttgaaat ccatgaccgg cacaccgcca ctggctgggt tcaagcgctc aagttcgggg
     1201 gccacaacgg tctgcagaat gaatctgcgg gcggtctcta accctccgtc caggtatagt
     1261 gcagcgagca cggcttcgaa agcatctacc aggagcgcgg tcttgctgcg gccgccgctt
     1321 ttttcttccc ctcggccgag gcgcaggtag cctcccaggt tcaagccttg cgccacctga
     1381 atgagatgcc gttcactgac caggtgggcg cgtaatttgg agagctcgcc ttcgcgaaac
     1441 gtcgggaagc ggcggaacaa ctcttcgccg gttacaaggg cgagtacggc gtcaccgagg
     1501 aactcaagct gctcgttgtc gccgggccgc acgccggttt tcaaaggctg caaagcttcg
     1561 agttcccgag cctgggaact gtgggtcagc gcctggtcga gaagttcggg gcgctcgaag
     1621 tgatatccca acaaaccttc cagcgccgca atttctttga ctttcatgca gattcggggt
     1681 cgaagctttc tgcgaacctc tcgagccccg tgagtggtgc atctttcgaa cccgtcagtt
     1741 cttcggctgc tcgggggtag ctggtttggg agcggcggcg ttgccgccga ccagttgatt
     1801 gagacgatcg cgctcctggc gcgcaagttt gccggcgctg gtggtttcct gcgtcagctc
     1861 cactgcgtgg ttagcttctt tcaacgcttc cggatatttg ttctgacggt cgagggaaat
     1921 cgcaagacgc aggactacca cggggtccgg ttgcgagggg aaagcatcga ttgacttgcg
     1981 cagatcggtt tccgccgcgg cgaagttatc tttcttgaac tccagggttc cgatgatgga
     2041 atacgcgaac gagcgcagct gtcccttgta gccatcgact tggtcttgcg gcgttccggg
     2101 cggaatcgaa atgtcggtgt caaccgtctg cagggacttc tgcgccatct tcatcgcttc
     2161 gtcaagacgc tgatctttgt cgagatcggt atcacgcgtc ttttcggcga gttcgctggc
     2221 gaccgtgacg agcagttccg gatcgtcgcc atcgatggcg agccccttgc ggcccatctc
     2281 aatgatcttg tcggagttgc cagccttttg gtaggtatgc atcgcggact tatacagcat
     2341 cacgcgcagc tcactgtccg ggaacttcgt ggcaaagtcg tcggcggcct tctcggccgc
     2401 ggcggcatcg gtcagcgcga ccgcggtttt gtacgcatcg tactcgggtt gcgtcttggc
     2461 ttgtggtgga cgcttgccct gcgccgctgc gggagcctgg gttccggttt gtgccgttcc
     2521 gggttgcgct gccttttgcg tgtcgggttt gctattggac tgcgccacgc cccacgtcgc
     2581 gaccgccagc acagcaccga tcatcgctac tcgcttcatg ctctctcctt agaatgacgc
     2641 tcacaaaaga atcgaagtgc ttacttcttg tctgactgct gccggcttgc gggaggctca
     2701 tacggttgct gcagcccacg ctccgcggcc gctttgactt cgggcagggt gccgccggcg
     2761 ttgagcgcgg catgatattc atccaacgcg ttctcacgcc gttcctgcag atcaaaaatc
     2821 ctgcccagat agatatgaga ccaggcaatc accttgggtt cctgtgccac tttcaaggct
     2881 tgttcgaaat atccggtggc gcccgccatg tcacggtttg ccgtggcgac ttgcgccagg
     2941 ataaacaacg cgcgtcccgc gtcttcatgg ccgtcatgca acgcttgctg ggccagttcc
     3001 tgagaagttt tcaaatcgcc cgccgccaga cgcttctccg ccagcacgag aagatgctgt
     3061 tgcttgggac gggcaaaatg taacacgtcg ggaaccgctg cagaggcgaa gtgcgtttcg
     3121 gctgcacgct tctgttcttt cttcaaatcg acattggcga gcaaatcgat gtaggcgttg
     3181 cggattcctt ccggcgcctt ttcgaattgc gcaagcgaat cataaaagta gcgcgtcagt
     3241 acgaatcctt gttccgccga tttctcgatc gcgtcgtcac gcagggcctc cggcgtcttg
     3301 ctcatccctt gcatccggat ctcgacggcg cgcaccaggc attccgtgac caagagggaa
     3361 gtgtcgatct tgaaggactc ttccatggga gcccccttga ccgattcgag cagaggctcc
     3421 agtcgtttca tcgatgtcgg gtatttcagc gacaacggat ccagcaggta gtgcaaatag
     3481 gtgtgccgga tctgatccat cttgagttcc tgaccagccg gtgaaattac cacgaagtag
     3541 tcgctgccgt aattacgcgc gttcacctgg ctgggtgcgc ccatgggctc caggaatact
     3601 gagaatccgc ggcccaggta tcccgctgac ggtaatttga ggtaaatctc ggt
//
LOCUS       DASTPM010000196         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_352976, whole genome shotgun sequence.
ACCESSION   DASTPM010000196 DASTPM010000000
VERSION     DASTPM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352976"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2222)
                     /locus_tag="VFR42_03465"
     CDS             complement(<1..2222)
                     /locus_tag="VFR42_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HEU0368242.1"
                     /translation="MGFRFESAGVETYPEPVEHCTVCRWFKECDTRRRQDDHLSLVAG
                     IRTQQRKQLEEWNVATLAELAKMPVPLKQRPRYGSRDGYERVREQARVQVQGRAENKP
                     VHEPILPVAQGTGFCRLPEPNRLDMFVDLEGDPFAGTQGQQYLFGLSARGADGELAYE
                     KRWSLTAAEEKSGFEWLMDEIVRRWAEEPGMHVYHYGANEPGAFKRLMGQYATREEEM
                     DRMLRGGKFVDLHRVVKQGVRASVEEYSLKKMEVFYRFVRKTSLDASRAAMRYIEHHL
                     ELGWKGEAPRDDMREAMEGYNREDCESTAALRNWLEAERAKLIRAGEKIARPVAGDPE
                     PPEELSERQKRVAELVQRLTKDLPVERADRSAAQQAHWLMAQLLDWHRREDKATWWEG
                     FRLAELDEDELLEERSGLGGLRHVATLRVEKQIRTDRYEFEKQETEVREEKELYYKGE
                     KFGTVVAVDFAARTVDVKKTKKSAELQPKGVYLWDAPLNVTQQADALLRVGEWVAANG
                     IERAGEFQAARDLMLRKAPRLGNDETVAVLPGELTEDAACRVGSAMRETAFAIQGPPG
                     AGKTYTGARMICDLVKRGKRVGVAALSHKVIRKLLEEVVKAALEKKIRGVCCLQRWNE
                     GEAEPGIGVAKKNEEIYATLQTRTASVIGGTSFLWSRGEMTDAVDVLFIDEAGQMALA
                     DLISVAHAAKSLVLIGDPQQLERPLKGSHPEGAAKSALEHLLGEHKTILPDVGLLLPE
                     T"
BASE COUNT          377 a          887 c          484 g          529 t
ORIGIN      
        1 gtctcgggca gcagcaatcc cacgtccggc aagatcgtct tgtgctctcc cagcaagtgt
       61 tccaaggctg acttcgccgc gccttccgga tgacttccct tcagcggccg ctccaattgt
      121 tgcggatcgc cgatcagcac caggcttttc gccgcgtgcg ccaccgaaat cagatcggcc
      181 agcgccattt gccccgcttc atcaatgaac agcacatcca ccgcatccgt catctctccg
      241 cgcgaccaca aaaacgaggt tccgccgatc acgctggccg ttcgcgtctg cagcgtcgca
      301 taaatctctt catttttctt ggccacgcca attcccggct ccgcttctcc ttcgttccac
      361 cgttgcagac aacacacgcc gcgtatcttt ttttccaaag ccgctttcac tacctcttcc
      421 agcaatttgc gaatcacttt gtggctgagc gccgccacgc caaccctttt cccgcgtttc
      481 accaagtcgc agatcatccg cgcgccggta tatgtttttc cggctcccgg cggcccttga
      541 atcgcaaatg ccgtttccct catcgcactc ccgacacggc aagcggcgtc ctccgtcagt
      601 tcgcctggca gcacagccac ggtctcgtca tttcccagcc gcggggcttt ccgcagcatc
      661 agatcgcgtg ccgcttgaaa ttcacccgcg cgctcgattc cgttcgccgc cacccattcg
      721 cccacgcgca acagcgcatc cgcttgctgc gtcacattca gcggagcatc ccacagatac
      781 actcccttcg gttgcagctc cgccgatttc ttcgttttct tcacgtccac tgtgcgtgcc
      841 gcaaaatcca ccgccaccac tgtcccaaac ttttctcctt tgtaataaag ctccttttcc
      901 tcgcgtactt ccgtttcctg tttttcaaac tcgtagcggt ccgtacggat ctgcttctcc
      961 acgcgcaacg ttgcgacgtg ccgcagcccg ccgagacccg accgctcctc cagcaattca
     1021 tcctcatcta gctccgccag ccggaaccct tcccaccacg tcgccttatc ctcacgccgg
     1081 tgccagtcga gcaactgcgc catcagccag tgcgcctgct gagccgccga tcggtccgct
     1141 cgttccaccg gcaagtcctt cgtcagtctc tgcaccagtt ctgcgacccg cttctgcctt
     1201 tcgctcaact cttctggcgg ttccggatcc cctgccaccg gccgcgcaat cttctccccc
     1261 gccctaatca gcttcgcccg ctccgcctcc agccaatttc gcagcgccgc cgtggattcg
     1321 caatcctctc ggttgtaacc ctccatcgcc tcgcgcatat cgtcccgcgg cgcctcgcct
     1381 ttccatccca gctccagatg atgctcgatg taccgcatgg ccgcccgcga agcatcgagc
     1441 gaagtcttcc tcacaaatct gtaaaacact tccatcttct tcagcgaata ctcttccacg
     1501 ctcgcgcgca cgccctgctt caccactcga tgcaggtcga caaattttcc cccgcgcagc
     1561 atccggtcca tctcttcttc tcgcgtcgcg tattgcccca tcaatctctt gaacgcgccc
     1621 ggctcattcg cgccgtagtg atacacgtgc atccccggct cttccgccca ccgccgcaca
     1681 atttcgtcca tcagccattc gaacccgctc ttttcttccg ccgccgtcag cgaccaccgt
     1741 ttttcgtacg ccagctctcc atccgccccg cgcgccgaca atccaaacaa atattgttgg
     1801 ccctgcgtcc cggcgaaggg gtcgccctcc aggtccacaa acatgtccag ccggtttggc
     1861 tccggcagcc ggcaaaatcc cgtgccttgc gccaccggca aaatcggctc gtgcaccggc
     1921 ttgttttctg cccggccttg cacctgcacg cgcgcctgtt ctcttacgcg ctcatacccg
     1981 tcgcgcgatc catatctcgg cctctgcttc agcggcaccg gcatctttgc cagctctgct
     2041 agcgtcgcca cattccactc ttccagttgc ttccgctgct gcgtccgtat tcccgccacc
     2101 agcgacaaat gatcgtcctg gcgtcgccgg gtgtcgcatt ccttgaacca tcggcacaca
     2161 gtgcaatgct ccaccggctc cggatacgtc tctacgcctg cagactcaaa acgaaaaccc
     2221 aactgaccat ccggtctctt ttcgccagac gcgtcactat ttacgccacc gcttttc
//
LOCUS       DASTPM010000197         3252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1246632, whole genome shotgun sequence.
ACCESSION   DASTPM010000197 DASTPM010000000
VERSION     DASTPM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246632"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFR42_03470"
     CDS             <1..630
                     /locus_tag="VFR42_03470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0368243.1"
                     /translation="QASSFQRVRVWGEILLVFFLLELIFWTPRSLAHSGLIVAAVGSS
                     IWLGFRGHRWEELGFVWPSRAGTLTILASGCVVAIAVPAVVLLSGHPVPANPDWPKFQ
                     NVWPYVLWAVGQQFLLQSFFYVRFESLLGTRAAVIVSTVLFALLHLPNLPLTAMTVLA
                     GLFFTEMFRTYRSLYPLGIAHAVMGIAIAYSFPDALMHHMRVGLSYWQF"
     gene            657..2321
                     /locus_tag="VFR42_03475"
     CDS             657..2321
                     /locus_tag="VFR42_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368244.1"
                     /translation="MEPDLTGPNFLSTKFENDIARQEATESTRRDAVRHGAPGSSWLR
                     FAVPSVADLVFVLLLVALSWGTLSKALLGDAGIGWHIRTGELILSTHAIPRVDPFSSV
                     MRGQPWYAWEWFYDGIVGVLHGVAGLNGVVLFSATVIALTFALVYRKMIESGAQLPVA
                     VVMLLLVFAASSIHFLARPHVLSWLFVVMWFGFLERFEADGNWRRLAWLPALMVLWVN
                     VHGGFLLGLALLGIYFVSALLSAWVWQGSEERDRAFGRARILAAVATASTVVTLANPF
                     GYKLHVHICRYLSDRFLMDHIDEFLSPNFHGFPQKCFAVIVLFAVAAMAGSRKHFRLS
                     HLLVVLFAIYSGLYASRNIPPSAILLTLIAAPQLSMAFSEAAGNRDVSELWRNRLARF
                     DRFGSKMVDLDRGLSGHVWPALALLVLVWACAHQGYIGTNHVLDAHFDDKRFPVDAVD
                     VLASGQSRGSVFCPDKWGGYVIYRLYPKTLVAVDDRHDLYGTAFLKRYLKIVHGEPGW
                     DGELMAMHADWVLVPADSTMASILSGSAGWKIAYRDDVAALFAGQP"
     gene            complement(2398..>3252)
                     /locus_tag="VFR42_03480"
     CDS             complement(2398..>3252)
                     /locus_tag="VFR42_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368245.1"
                     /translation="GGIQYHSLLLPAKQPVQMDYAFADGSLILSSSHEAAAEAIALHQ
                     HGESLAKSKNFLPSYPSGRSHEASALLYEDAFALMSARMQQLPLEIAQPISQVQPGSA
                     PVTFWGYGEDTAIRGISSNAGADAGVILVGAAIAIPNLLRARIAANESSAAGNLRTIN
                     TARITYSVAYPQREYAPDLATLGMDPHNPAAMSITHAGLIDTPLGDAGCVAGTWCLKS
                     GYRFMVSAVCKVRACKEYVAIATPDSANTGTKNFCSTSDGLIRLQVGQPLSKVITAPE
                     CQEWPPIQ"
BASE COUNT          549 a          864 c         1071 g          768 t
ORIGIN      
        1 caagcgtcga gctttcaacg agttcgtgtc tggggagaaa tccttctcgt cttcttcctg
       61 ctggagctaa ttttctggac gccgcgcagc ctggcacaca gtggcttgat tgtagccgcg
      121 gtcggcagtt cgatatggct aggcttccgg ggacaccgct gggaagaatt gggattcgtg
      181 tggccttcgc gcgcggggac cttgacaatt ctcgcgagcg gctgcgtggt ggcgattgcg
      241 gttccggcag tggttctgtt gagcggccat ccggtaccgg cgaatccgga ctggccaaag
      301 ttccaaaatg tctggccgta tgtactttgg gcggtggggc aacaatttct gttgcagtcg
      361 ttcttctacg tgcgctttga atcgctgctg gggacgcgcg cggccgttat cgtcagcacc
      421 gtcctgtttg ccctgcttca tcttccaaat cttcccctga ccgccatgac ggtgcttgcc
      481 ggtctgtttt tcacggagat gttccggacg tatcgcagcc tgtatccgct cgggatcgcg
      541 cacgccgtga tggggatcgc catcgcttac agctttccgg atgccctcat gcaccacatg
      601 cgggtaggac tgtcgtactg gcagttctaa gtggattttt ctctccttcg attaccatgg
      661 aacccgatct gaccgggccg aacttcttga gcacaaaatt tgagaacgac atagcgcggc
      721 aggaggcgac agagagcaca cgccgcgatg ctgttcgcca tggcgcgccg ggttcttcct
      781 ggttgcgctt cgccgttcca tcggtggccg acctcgtgtt tgtgctcttg ctggtggcgc
      841 tcagttgggg aacgttatcc aaagcattgc tcggcgatgc gggcattggg tggcacatcc
      901 gcacgggaga gttgattctc agcacacatg cgatcccgcg agttgaccca ttttcctccg
      961 tgatgcgggg acagccctgg tatgcatggg agtggtttta cgacgggatc gtgggcgtgt
     1021 tgcacggagt cgccgggctg aatggcgttg tgctgttcag tgccacagtg atcgcgctga
     1081 cgtttgcgct ggtgtatcgc aagatgatcg agagcggagc gcaactgccc gtcgcggttg
     1141 taatgttgct gctggtgttt gccgcgtcaa gcattcactt cctggctcgt cctcatgttt
     1201 tgagctggct gttcgtggtg atgtggtttg gttttctgga gcgcttcgag gccgacggca
     1261 attggcgaag gctggcttgg ctaccggcgc tgatggtgtt gtgggtaaac gtgcatggcg
     1321 ggttcctgct gggattggcg ctgctgggga tctacttcgt gagtgctttg ctcagcgcgt
     1381 gggtctggca ggggagtgag gaacgggatc gggcgttcgg gcgtgcgcga attttggctg
     1441 ctgtagccac ggcctcgaca gttgtgaccc ttgccaatcc gtttgggtac aagctgcacg
     1501 ttcatatctg ccgttacttg agcgaccggt ttctgatgga tcatatcgac gaattcctct
     1561 cgccgaattt tcatgggttc ccgcagaaat gctttgcggt cattgtgctg tttgcggtgg
     1621 cggccatggc gggatcgcga aaacacttca gactcagcca tctgctggtg gtgttgtttg
     1681 cgatttactc gggattgtac gcgtccagaa atattccgcc ttcggcgatt ctgctgacgc
     1741 tgatcgcggc gccgcagctt tcgatggctt ttagcgaggc ggccgggaac cgggacgtga
     1801 gcgagttatg gcgcaaccgc ctggcgcgct tcgatcgatt tggctcaaag atggtggatc
     1861 tggatcgcgg cctgtcgggg cacgtgtggc cggcactcgc attacttgtg ctggtgtggg
     1921 cttgcgcaca tcaagggtac atcggaacga accacgtgct ggacgcgcat tttgatgaca
     1981 agcggtttcc cgtggatgcg gtggacgtgc ttgcgagcgg gcagagccga ggctcggtct
     2041 tctgccctga caagtggggc gggtatgtga tttaccggct ctatccgaaa acgctggtcg
     2101 cggtggatga ccggcacgat ctgtatggaa cggcgttctt aaagcgttac ttgaagattg
     2161 tgcacgggga gccggggtgg gatggagagc tgatggcgat gcatgctgac tgggtgctgg
     2221 ttccggcgga ttcgacgatg gcgagtattc tcagcggcag cgccggctgg aagattgcgt
     2281 atcgagatga cgtcgcggct ctttttgcgg ggcagccgta gcgcggctgc accctcttga
     2341 cccgcgggaa ggcccgcacc acattgcggg ctcggattac gccgacgatc ggcctggcta
     2401 ctgaatcggc ggccactcct gacactctgg cgccgtgatg actttgctca gcggttggcc
     2461 cacttgcaag cggatcagcc catcggaagt ggagcagaag ttcttggtgc cggtgttggc
     2521 cgagtctggg gtggcgatcg ccacgtattc cttgcacgcg cgcaccttgc aaactgccga
     2581 gaccatgaag cggtatccgg atttcaaaca ccacgtaccc gcgacacagc cggcatcgcc
     2641 tagtggcgta tcgatcaaac ctgcatgagt aatcgacatg gcggctgggt tatggggatc
     2701 catgccgagc gtcgccaggt cgggcgcata ctctctctgc gggtacgcga ccgaataagt
     2761 gatccgtgcg gtgttgatgg tgcgcaagtt gccagccgca gaggactcgt tggcagctat
     2821 tcgcgcacgc agcagattgg gaatggcaat ggcggcgccc accaggatta cacccgcatc
     2881 cgcacccgcg ttcgaactga tgccgcggat ggccgtgtct tcgccgtagc cccagaaggt
     2941 gacgggcgca gagccgggtt gcacttggga aatcggctgc gctatctcaa gtggcagttg
     3001 ctgcatcctc gccgacatca aggcgaacgc gtcctcatac agcagggcgg acgcctcatg
     3061 cgaacggcca gacggatagg acggcagaaa attcttggat tttgccagcg actctccgtg
     3121 ctggtggagc gcgatcgctt cggcagcagc ttcatggctc gaactcagga tcagggaacc
     3181 gtcggcgaag gcgtagtcca tttgaaccgg ttgcttcgca ggcagcagga gggagtggta
     3241 ttggattccg cc
//
LOCUS       DASTPM010000198         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_93321, whole genome shotgun sequence.
ACCESSION   DASTPM010000198 DASTPM010000000
VERSION     DASTPM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93321"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VFR42_03485"
     CDS             complement(<1..924)
                     /locus_tag="VFR42_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU0368246.1"
                     /translation="MPTDRKPEFFKGAQLKVGIIGCGYVGLPLALRFAEAGHKVTGFD
                     TDPSKVSMLNAGKSYIEHIRQDKIQQFVNSRHFSATTDFERLKDVDAIIICVPTPLDE
                     RREPDLSYVEQTAVSIQPNLQKGQLVVLESTTYPGTTEELVLPILEKTGLRCPIAAGI
                     ESDNIPTDFFLAFSPEREDPGNKQYGLAQIPKVVGGTNPPSARAAQALYAQVVAKVVP
                     VSSTRAAEMAKLLENIFRCVNIALVNELKLLASRMNIDIWEVIDAAATKPFGFMPFYP
                     GPGLGGHCIPVDPFYLSWKAREYDFSARFIEL"
     gene            1186..1596
                     /locus_tag="VFR42_03490"
     CDS             1186..1596
                     /locus_tag="VFR42_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0368247.1"
                     /translation="MTTILVLALGVVAGILLGLLGIGGGVVLVPAMVHVLHMDQHLAQ
                     GTSLFILLPPIGLGALRQYWKAGHVDLRAGILCAVGMLLGAWIGGLIAVPMSTPALKV
                     WFGGYLIFSAVLLWMKATPAQKVPVPASLKEGGA"
     gene            1593..1976
                     /locus_tag="VFR42_03495"
     CDS             1593..1976
                     /locus_tag="VFR42_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0368248.1"
                     /translation="MNRKILLDVGVLFTGLAGGMASGLFGIGGGTIMVPILGLLLGFT
                     QHRAQGTSLVALIPPTGLLAVLAYGKAGYLDWRTGLLLVPGVFLGGVAGGNLAKRIPS
                     VAMRKIFAALMFLLGVYQIYSSVRG"
     gene            1991..2758
                     /locus_tag="VFR42_03500"
     CDS             1991..2758
                     /locus_tag="VFR42_03500"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEU0368249.1"
                     /translation="MVDLHCHILSGMDDGARSFEESVAMAEMAIADGITHVVATPHAS
                     QEFKFDYANVRRSAEELQKAVGDRLKLATGCDFHLSPENVAALKKAPSPFCIHQKDYL
                     LVEFNEFAIPPQSDQTLYELQLAGLRPIITHPERNGILRAQPERFEKWVRNGCYGQVT
                     AGSLTGTFGPNAQTASMEWLARGLVHFIASDAHNTTARPHKLRAAYELVRKEFGSGKA
                     EALFVENPLAAFEGRGLPHVPEISDVELKKKRRFIFF"
     gene            complement(2760..3434)
                     /locus_tag="VFR42_03505"
     CDS             complement(2760..3434)
                     /locus_tag="VFR42_03505"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU0368250.1"
                     /translation="MNDPMNDTPPRFCLSRRAFYWLGGCNVVLFLLAVGAFFHAGTWL
                     VREDAVQKAQIVVVLSGGLPERALAAAEVFKASGAREVWLTRTLQPGAAMQQLHLPYA
                     GEEQYSRMVLIDRGVPPAAIRALTPQINNTADELKAVFDELNSQPETTVVVVTSKAHT
                     RRVRAIWNVVSRRSTRGNLWVRAAPQDGFDAEHWWRSTNDAWSVVHEYLGLLNAWVGL
                     PIHYMR"
BASE COUNT          729 a          907 c         1203 g          767 t
ORIGIN      
        1 gagttcgatg aagcgtgctg agaagtcgta ctcgcgggcc ttccaggaga gatagaaagg
       61 gtcgactggg atgcagtggc cgccgaggcc cgggccggga tagaacggca tgaaaccgaa
      121 gggtttggta gcggcggcgt cgataacttc ccagatatca atgttcatgc gggaagcgag
      181 gagcttgagt tcgttgacca gagcgatgtt gacgcagcga aagatgtttt cgagcagctt
      241 ggccatttcg gcggcgcgag tggagctgac cgggacaact ttggcgacca cttgtgcgta
      301 aagggcttgc gcggcgcgag cgctgggcgg gttagtgccc ccaacgactt tgggaatctg
      361 cgccaggccg tattgtttgt tgccgggatc ttcgcgttcg ggagagaagg cgaggaagaa
      421 gtcggtcgga atgttgtcgc tctcgatgcc ggcggcgata ggacagcgca ggccggtttt
      481 ttcgaggatg ggtaggacga gttcttcggt ggttccggga taggtggtgg attcgaggac
      541 gacgagctgg cccttttgca gattgggctg gatggagacg gcggtttgtt cgacgtagct
      601 gaggtcgggt tcgcggcgtt cgtcaagcgg agtgggaacg cagatgatga tggcgtcgac
      661 atccttgagg cgctcgaaat ccgtggtggc gctgaaatgg cggctgttca caaactgctg
      721 aattttgtcc tggcggatgt gctcgatgta tgacttgccg gcgttgagca tggaaacttt
      781 ggaaggatcg gtgtcaaagc cggtgacctt gtggccggct tcggcgaagc gcagagcgag
      841 tgggagccct acataaccgc acccaataat tccaactttt aactgcgcac ctttgaagaa
      901 ttctggtttc cggtcggtag gcatctgaac ttgatcgctc ctcgattagt ggatcagggt
      961 tcgtgagtac aagctggaaa accctgaaag aatgaattga agaaacacgc gcacagggcg
     1021 cgaagggctg cactatcaat ataacaggtt cgccgcgcag gccggccggg caggtgatgt
     1081 gtatgttacg cgttgttcaa ggttgtgtgg cattgcctcg gacaggattg ccgcagagaa
     1141 tggacacgtg tgcgatagcg aaggagaacg ccgcggagcg ttgatttgac gaccattttg
     1201 gtattggcgt tgggagtcgt agcgggaatt ttgctgggct tattaggaat tggaggcggc
     1261 gtcgttctgg tgccggccat ggtccatgtt ctacacatgg accagcacct ggcgcagggg
     1321 acttcgctgt ttatcctgtt gcctccgatt gggctgggtg cgctgcggca atactggaag
     1381 gcggggcatg tcgacttgcg cgcgggaatc ttgtgcgcgg ttgggatgct tttgggcgca
     1441 tggatcggcg ggctgattgc ggtgccgatg tccacgccgg cgctcaaggt gtggtttggc
     1501 ggatacctga tattttccgc ggtgctcctg tggatgaaag ccacgccggc acaaaaggtt
     1561 ccggtacccg cctcgttgaa agagggcggc gcgtgaatcg caaaattctt ctggatgtgg
     1621 gagtgctgtt tactggattg gccggaggga tggcgtcagg gctgttcggg atcggcggag
     1681 gaaccatcat ggtgccgatt ctgggattgt tactgggctt cacgcagcat cgcgcgcagg
     1741 gaaccagcct ggtcgcgttg attccgccca cggggttgct ggccgtgctg gcgtacggta
     1801 aagcgggcta cctggactgg cgaacgggtt tgctgctggt ccccggagtc ttcctgggcg
     1861 gcgtcgccgg tggaaatctg gcgaagcgca ttccatcggt ggcgatgcgg aaaatatttg
     1921 cggcattgat gttcctgctc ggcgtgtatc agatttacag ttcagttcgc ggatgaaggc
     1981 cgcgtgagcg atggttgact tgcattgtca catcctgagc ggtatggacg atggagcgag
     2041 atcgttcgaa gaatcggtcg ccatggcgga gatggcgatc gctgacggca tcacacacgt
     2101 tgtggctacg ccgcacgcca gccaggagtt taagttcgac tatgccaacg taaggcggag
     2161 cgctgaggaa ttacagaagg cggtcggcga tcgcctgaag ctcgctaccg ggtgcgattt
     2221 tcacttgagc ccggaaaacg tcgccgcact gaaaaaagct ccgtcgccgt tttgcatcca
     2281 ccagaaagat tacctgctgg tggagttcaa cgagttcgcc attccaccgc agtcggacca
     2341 aacgctttac gagcttcagt tggcggggct gcggccgatc atcacgcacc ccgaacgaaa
     2401 cgggattctt cgcgcgcagc cggaacgttt tgagaagtgg gttcgcaacg gctgctacgg
     2461 gcaagtcact gctggctcgc tgaccggcac gtttggaccc aacgcgcaga ctgcgtcaat
     2521 ggaatggctt gcgcggggcc tggtgcactt catcgccagc gatgcccaca atacgacggc
     2581 gcgaccgcac aaattgcgcg ccgcgtatga gttggtgcga aaggaatttg gctcgggaaa
     2641 agctgaagcg ctgtttgtcg aaaatcctct ggcggctttc gaagggcgag gattgccgca
     2701 cgtgccggag atcagtgacg tcgagttgaa aaaaaagagg cgtttcatat ttttctaagt
     2761 cagcgcatgt aatgaatggg aaggccgacc cacgcgttga gcaggccgag atattcgtgc
     2821 acgacgctcc aagcgtcgtt cgtggaacgc caccagtgct cggcgtcgaa cccgtcctgc
     2881 ggagcggcgc gaacccacag gttgccgcgc gtgcttcggc gcgaaacgac attccagatc
     2941 gctcgaaccc gccgggtatg cgccttggac gtaactacaa ctaccgtcgt ttccggttgc
     3001 gaattcaatt cgtcgaacac ggccttgagc tcgtcggcgg tgttattgat ttgcggcgtc
     3061 agcgcgcgga tcgcggccgg cgggacgcca cggtcaatca ataccattcg gctgtattgt
     3121 tcttcgcccg catagggcaa atgaagttgc tgcatcgcgg cgccaggctg tagcgtacgc
     3181 gtcagccaaa cttcgcgcgc cccgctcgct ttaaaaactt cggccgcggc caaagcacgc
     3241 tcgggcagcc cgccactgag caccacaaca atttgggcct tttgcacggc gtcctcgcga
     3301 accagccacg tgccggcgtg aaaaaatgcg ccaaccgcca gcaagaacag aacaacgttg
     3361 cagccaccga gccaatagaa agcgcggcgc gacaggcaga atcgcggcgg agtgtcattc
     3421 atcggatcgt tcatcgtgaa ccgcgcatgg tagttccgcc gggcacgagt ggtcaatcac
     3481 tccaggcaaa gatacactga caaagggaat tttttctgga ggaagtatgc gggcagtggt
     3541 aaccggagcg gcgggattcc tggggtcgca cctatgcgac cgttttctgg cggaaggatg
     3601 gaacgt
//
LOCUS       DASTPM010000199         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_410753, whole genome shotgun sequence.
ACCESSION   DASTPM010000199 DASTPM010000000
VERSION     DASTPM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410753"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2221)
                     /locus_tag="VFR42_03510"
     CDS             complement(<1..2221)
                     /locus_tag="VFR42_03510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0368251.1"
                     /translation="MIGQTISHYRVVEKLGGGGMGVVYKAEDTRLHRFVALKFLPDEV
                     SRDPQALSRFQREAQAASALNHPNICTIYDIGDQDGRAFIAMEFLEGVTLKHALSGRA
                     METDTLLALGIEISEALEAAHAKGIVHRDIKPANIFVTSRGHAKILDFGLAKRTATSN
                     SSSKAGATSADTLDSGHLTNPGTTIGTVAYMSPEQTRGKELDPRTDLFSFGTVLYEMA
                     TGIMPFRGDTSALIFNAILEKEPTPPLRLNPDLAPKLQDIIEKALEKDRALRYQSAAE
                     MHADLARLKRDTASRAVPAAMGHDSAEKAPASANLQASASAAANATPAPVVSSASVRG
                     PQTKSGSTTITVPTWLASRGVWGGVAAAVILLAGGIFFWQRPARGLSEKDAILVTDFT
                     NTTGDSVFDGTLKSAAAVGLGQSPYLNVVSDQKILQTLKLMGQPAGARITPEIGRQIC
                     SRNAIKAMMTGSIASIGSQYIITLNAVNASTGDNIAQEQLQASKKDDVLSTLGTAVTN
                     LRGKLGESLASVKKFDKPLDEATTSSLDALKAYTEGSALRSKGNEAAAVPLLKHAIEL
                     DPNFASAHAILGTTYSNLGQVGLYEKYLKQAFDLKDRSSERERLYIAGHYYDSIGDIN
                     QELQTWQLYSQTYPNDEIPTSNLAVLYARLGDYETGLKSSLRGIQLAPDSNYGYLNAA
                     WAYDALGRFEEAKAIANESIKKVGETPIAHTMLLTTAIAQGDTAAEEKELALAAEDSG
                     "
BASE COUNT          428 a          579 c          766 g          521 t
ORIGIN      
        1 caccggaatc ttccgccgcg agcgccagct ctttttcctc ggcggcggta tcgccttgtg
       61 cgatggcggt ggttaacaac atggtgtggg caataggcgt ttcgcctact ttttttatgc
      121 tttcgttggc gatggctttg gcttcttcga agcggccaag agcgtcataa gcccaggcgg
      181 cgttcagata gccatagttg gaatccggtg cgagttggat accgcggaga gaagacttca
      241 ggccggtttc gtagtcgccg agacgagcgt agagaaccgc aagattagag gtgggaatct
      301 catcattcgg ataggtttga gaatataact gccaggtctg cagctcctgg ttgatgtcgc
      361 cgatggaatc ataatagtgg ccggcgatgt agaggcgttc gcgttcgctg gagcgatcct
      421 tgagatcgaa agcctgcttt agatattttt cgtagaggcc gacctggccc aggttgctgt
      481 aggtggtgcc gagaatggcg tgagcggagg cgaagttcgg atcaagttcg atggcgtgct
      541 tgagcagcgg gacggctgct gcttcgttgc ctttgctgcg gagcgctgat ccttcggtgt
      601 aagccttgag agcatccagc gaagaagtag tggcttcgtc gagcggcttg tcgaattttt
      661 ttacggaggc gagcgattcg ccgagtttgc cgcgcaagtt ggtcacagcg gtgccgagcg
      721 tgcttaggac gtcgtccttc ttggaagcct gcaattgttc ctgggcgatg ttgtcgccgg
      781 ttgaggcgtt caccgcattc aaggtgatga tgtactggct gccgatggag gcgatggagc
      841 ccgtcatcat tgctttgatg gcgttgcgcg aacagatctg gcgaccaatc tccggcgtga
      901 tgcgcgcgcc cgcaggctgg cccatgagct tcagagtctg caggatcttc tggtcggaga
      961 cgacgttcaa atagggggac tggccgagac cgacggccgc cgcgctcttt aacgtgccgt
     1021 caaacacgga gtcgcccgta gtgttggtga aatcggtaac cagaatggcg tctttttcgc
     1081 tgagaccgcg agcggggcgc tgccagaaga agattccacc cgctaacagg attactgccg
     1141 ctgcgactcc gccccagacg cctctgctgg ccagccacgt ggggactgtg atggtggtgg
     1201 aaccggattt ggtttgcgga ccgcgtacgg aagccgagga tacaacaggc gcaggcgtgg
     1261 cgtttgccgc cgcagaagct gaagcctgca gatttgccga agccggcgct ttttccgccg
     1321 aatcgtgtcc catggccgcg ggaactgcgc ggctggcggt atcgcgtttc aggcgcgcca
     1381 ggtcagcgtg catttccgcc gcgctttggt agcgcagggc acgatctttc tccagagctt
     1441 tttcgatgat gtcttgcagc ttgggcgcta aatccggatt caagcggagg ggcggcgtag
     1501 gctccttttc caggatggcg ttgaaaatca gcgcggatgt atcaccgcgg aacggcatga
     1561 ttccagtggc catttcgtac aacacggtac cgaaggaaaa gaggtcggta cgaggatcga
     1621 gttccttgcc gcgagtctgc tcaggcgaca tgtaagcgac tgtgccaatg gtcgtgccgg
     1681 gattggtgag atggccgctg tccagggtgt cggcggaagt cgcgcccgct ttgctggagg
     1741 agttcgacgt agcggtcctt ttcgccaggc cgaaatcgag aattttggcg tgaccgcggc
     1801 tggttacaaa aatgttggct ggcttgatat cgcggtggac gatgcctttt gcatgcgcgg
     1861 cttcaagcgc ttccgaaatt tcgatgccga gggcgagcag ggtgtcggtt tccatcgccc
     1921 ggccggagag tgcgtgtttc aatgtgacgc cttcgagaaa ttccatggcg atgaaggcgc
     1981 gaccgtcctg gtcgccaatg tcgtaaatgg tgcagatgtt gggatggttc agcgccgatg
     2041 cagcctgcgc ctcgcgctgg aagcggctga gggcctgggg atcgcgggag acttcatcgg
     2101 gcaggaattt gagggcgaca aatcgatgta agcgggtgtc ttctgccttg tagacgacac
     2161 ccatcccgcc cccgccgagt ttttcgacaa cgcgatagtg cgagatggtt tggccgatca
     2221 tgcgctgaca tgttaggagt gaaggtgcag caagtcaacg cgaagctcgg tcatgcagcg
     2281 ggaggctcac agcc
//
LOCUS       DASTPM010000200         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1419836, whole genome shotgun sequence.
ACCESSION   DASTPM010000200 DASTPM010000000
VERSION     DASTPM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419836"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2110
                     /locus_tag="VFR42_03515"
     CDS             <1..2110
                     /locus_tag="VFR42_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0368252.1"
                     /translation="MTGHLNVPALEPDPDTPATLSSYILTDLLRTDLHFQGLVVTDAM
                     DMGGITVRFAPGEAAVRAVLAGVDCVLMPPVPDAAFEALQQAVKSGRISRERLDASVR
                     RILEAKARVGLNKNRLVDLGAINSKFAEPAWQAQAQQISDHGITLLRDTPHRLPLDGS
                     KPSRALLLALYADPEPTPGEDLERELSRRFDSVSTVRADTRFRSAANLKLPPPDSYDV
                     AILALFVRVSDRKGNVDVPEEQAALAHQLYKSGKPVITLALGSPYLIERFPQAETWLS
                     AFGISDVAQISMARALFGEIPVRGHLPVTIPGIPLKAGFGLETPADPMKLQSMDAQSE
                     AQLQPAFDVVEAAIKDKAFPGAALAVGYRGKVSLHAFGKLTYDSKSPEVSLNTRYDIA
                     SLTKVVATTTLVAKLAEGDFPVPIDLDAQVERYLPEWASGPQPEWRHRVTVRHLLTHT
                     SGLPPFKEYWRTSKSKQDTLDKIFVEPLEYEPGTKEVYSDLGIILMAEIIERLTGKPL
                     DVLAHDDIFAPLAMANSMYKPPKKLWPAIAPTEIDNQYRHRLIQGEVHDENAAAIGGV
                     SGHAGVFSTAPDLAAFCQMLLNGGVYAHQRILRRSTIAEFTTPQQLSNGTRTLGWAAP
                     TEGGSSGHFFSPHSFGHTGFTGTSIWIDPDRQLFVVFLTNRVYPTRDNQKHIKVRPAL
                     HDAVVQALGLASVAEPATNR"
     gene            complement(2112..>2588)
                     /locus_tag="VFR42_03520"
     CDS             complement(2112..>2588)
                     /locus_tag="VFR42_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368253.1"
                     /translation="KKAAWFFLEDAGDEPFLLHNVDVISAIDLRQMVGEHKEAGALAT
                     LAVQERKSSRQLLFDEHGQLCGRRLGKDSADEIARSSAQLTPLAFSGIHVISPRFLKM
                     MTEEGVFSIIETYLRLASGGEKIQEFRADQYYWRDLGRPENIQQVEQDLGQKIIAY"
BASE COUNT          523 a          871 c          655 g          539 t
ORIGIN      
        1 catgaccggc cacctcaatg tccccgccct cgagccggat ccagacacgc ctgctaccct
       61 ttcgtcttac atcctgacag accttctccg cacggacttg cactttcaag gtctggttgt
      121 caccgacgcg atggacatgg gtggaatcac ggtgcgtttc gctccaggcg aagccgcggt
      181 tcgcgcggtt ttggctggcg tcgattgcgt gctcatgcct ccagtgcccg acgccgcctt
      241 cgaagcgttg cagcaggccg taaaatccgg ccgcatttcg cgcgaacggc tcgatgcttc
      301 ggttcgccgc attctcgaag ccaaagctcg cgtcggcctg aacaaaaatc gcctggtaga
      361 cctcggcgct atcaattcca aatttgccga accggcgtgg caagcccaag cccagcaaat
      421 ctccgatcac ggaatcactt tgctgcgcga cacgcctcat cgcctcccgc tcgacggaag
      481 caaaccgtct cgtgccctcc tgctcgctct ctatgctgat cccgagccca ctcccggcga
      541 agatctggag cgcgaactga gccgtcgctt cgattctgtc agcacggtgc gcgccgacac
      601 gcgttttcgc agcgccgcaa atctaaagtt gccgccgccg gattcctacg atgtggccat
      661 cctcgccctg ttcgtgcgcg tcagcgatcg caaaggcaac gtcgacgttc ccgaagagca
      721 ggccgctctc gcccatcaac tctacaagtc cggcaagccg gtcatcaccc tcgcgctcgg
      781 cagcccttat ctgattgaac gtttccctca agccgaaaca tggctgagtg cattcggcat
      841 cagcgacgtc gcccaaatct ccatggcccg cgcactgttc ggcgaaattc cagttcgtgg
      901 ccacttgcca gtcacgatcc ctgggattcc tctcaaggcg ggcttcggcc ttgaaactcc
      961 tgccgatccg atgaagcttc agtccatgga tgctcagagc gaagcccaat tgcagccagc
     1021 cttcgacgtt gtggaagccg ccatcaagga caaagcgttc cccggcgccg ccctcgccgt
     1081 cggctatcga ggaaaagttt cgctccacgc cttcggcaag ctcacctatg actcaaaatc
     1141 ccccgaggtc tccctcaaca ccaggtacga catcgcttcg ctcaccaaag ttgtcgcgac
     1201 aacaacgcta gtcgccaagc ttgccgaggg cgacttcccc gttcccattg acctcgatgc
     1261 ccaggtcgaa cgctatctcc ccgaatgggc cagcggtccg cagcccgaat ggcgccatcg
     1321 cgtcacggtt cgccatctgc tcacccacac ttccggcttg ccgcccttca aggaatattg
     1381 gcgcacctca aagtcgaaac aagacacgct cgacaagata ttcgtggagc ctcttgaata
     1441 cgaacccggc accaaagagg tctattcaga cctgggcatc atcttgatgg ccgaaattat
     1501 cgagcgcctc acaggcaagc cgctggacgt tctggcgcat gacgacatct ttgcgccgct
     1561 ggcgatggcg aactccatgt acaagccgcc gaagaaactt tggccggcca tcgccccgac
     1621 ggaaatcgac aaccagtacc ggcatcgttt gattcaaggt gaagtgcacg acgaaaacgc
     1681 cgcagcgatc ggcggcgtgt cgggccacgc gggcgttttt agcaccgcac cggaccttgc
     1741 ggcgttctgc cagatgctgc tcaacggcgg tgtttacgcg caccagcgta ttcttcgccg
     1801 ctcgaccata gcggaattca ccacgccgca gcaactttca aatggtacgc gtaccctagg
     1861 ctgggcggct cccacagagg gaggcagcag tggccatttc ttcagcccgc attcctttgg
     1921 ccacaccggt ttcaccggca cttccatctg gatcgatccc gatcgccagc tttttgtggt
     1981 ttttcttacg aaccgcgtct atcctacgcg cgataaccag aaacacatca aggttcgccc
     2041 cgcgctgcac gatgctgtcg tgcaggcgct gggacttgca tccgtcgcgg agcccgcgac
     2101 caatcgctga gttagtacgc gattattttc tgtcctaagt cctgctcgac ttgctggata
     2161 ttctctggtc tacccagatc gcgccagtag tattgatcgg cacgaaactc ctgaatcttc
     2221 tcgccaccac tggcgagtct caagtaggtc tcgatgatcg agaacactcc ttcttcggtc
     2281 atcatcttca ggaatcgcgg ggaaatcaca tgaattccag aaaatgccag cggcgtcagc
     2341 tgtgccgagg atcgcgcgat ctcatcggcg gaatctttcc ccaagcgccg tccgcaaagc
     2401 tggccgtgtt cgtcaaacag cagttgccgc gaacttttcc gctcctgcac cgccagagtc
     2461 gccagagcac cggcctcctt gtgctcccct accatctgcc gtaaatcgat cgcgctgatc
     2521 acatccacgt tgtgcagcaa aaagggttcg tcgcccgcat cttccagaaa aaaccacgca
     2581 gctttttt
//
LOCUS       DASTPM010000201         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_756461, whole genome shotgun sequence.
ACCESSION   DASTPM010000201 DASTPM010000000
VERSION     DASTPM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756461"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFR42_03525"
     CDS             complement(<1..937)
                     /locus_tag="VFR42_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368254.1"
                     /translation="MTAILGMLASERLVKMSPVDLGIVVFYFALVLGIGFYLKRFAKT
                     GEDFFLAGREMTAWVAGLAFVSANLGSLELLGWAGSAYQYGILAAHWYWIGAIPAMIF
                     LGLVMMPFYYISKTHSVPGYLQLRYGPEASGLSAITFALMTILMSGVNMYAMAVVMKV
                     VLGWDINFSIWVSSLTVGVYVAAGGLFSAIFNEVLQFFLIWFGALLIPILGLIETGGW
                     KGMVARIQHNFPGQDFTHMWGPMHSFSSNPMGIHWTGIVFGLGAVQAMGYWTTDFLVV
                     QRVLSAKDIRSAKMAPIIGSFFKMAVPLIVILPGLL"
     gene            complement(1467..2618)
                     /locus_tag="VFR42_03530"
     CDS             complement(1467..2618)
                     /locus_tag="VFR42_03530"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HEU0368255.1"
                     /translation="MNGRTIAICCLAAFPFLALMVIPTDLRAEANPPEGAQKVEKKSF
                     GKLPDGTEIEEYTLHSAKGAGAKVITYGATLTELRVPDKAGKNADVVLGFDSLAAYTG
                     DHPFFGATIGRYGNRIAKGKFSIDGHEYSLFLNNGPNSLHGGKEGFNRKVWTAELVKA
                     NHGAAVKFTYVSKDGEEGYPGTLTTVVTYELTDDNALKLTYHASTDKPTVVNLTNHSY
                     FNLSGAGSGDILNEVLQLDADRFTPVDATLIPTGELKSVEGTPFDFRKPTAIGERNAQ
                     VPGTGGYDHNFVLNGTSGKLRKIGSVSDPASGRAMEIWTTEPGVQLYVSLGLNGSLKG
                     IGGAYAKFGALCLETQHFPDSPNHPDFPSTVVRPGQDYHSETIYKFSAK"
     gene            complement(2710..>3483)
                     /locus_tag="VFR42_03535"
     CDS             complement(2710..>3483)
                     /locus_tag="VFR42_03535"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HEU0368256.1"
                     /translation="RAHGTRTTNQNEIWSYGPQMQAILERYDRLRYRLLPYIYSVAWK
                     TTSENYTQMRALAMDFRDDPRVLNIGDQYLFGPSLLVNPVTEPGATTRHLYLPKGNWY
                     NFWTGESVEGGRAIDTPAPLEEMPIFVRAGAILPMGPTIEYAQQPSNDPLEIRVYTGA
                     DGDFTFYEDDGDTYDYEKGAFATIPIHWNEATHRLIIGARKGSYAGMPQTLPLVVVFV
                     RKGTGIGFEEAKFAPREVIPYDGRMVEVTLSENLSGSDR"
BASE COUNT          752 a          979 c         1037 g          715 t
ORIGIN      
        1 ccagcaaacc tggaagaatc acgatcagcg gcacagccat tttgaaaaat gatccgatga
       61 tcggcgccat tttcgccgaa cgaatatcct tagccgacaa cacgcgctgc accaccagga
      121 agtccgtggt ccagtagccc atggcctgca ccgcgccgag tccgaacaca attcccgtcc
      181 agtgtatgcc catcgggttg ctggagaatg aatgcatcgg gccccacatg tgcgtgaagt
      241 cctggcccgg aaaattgtgc tggatgcgcg cgaccatgcc tttccatccg cccgtttcaa
      301 tcaagccgag tatcgggatt agcagcgcgc caaaccagat caggaaaaat tgcagcactt
      361 cgttgaagat cgcggagaac aaaccgccgg cagcgacata cacgccgact gtcaatgacg
      421 aaacccagat gctgaaattg atgtcccagc cgagaactac tttcatcacc acggccatgg
      481 cgtacatatt cacgccggac atcagaatgg tcatcaacgc gaatgtgatg gcgctgagcc
      541 cgctggcttc cgggccataa cgcaattgca gatagcccgg caccgagtgc gtcttggaga
      601 tgtaatagaa cggcatcatc accaagccga ggaaaatcat ggcggggatt gcgccgatcc
      661 aataccagtg tgcggccagg atgccgtact gataggccga gcccgcccag ccaagcagtt
      721 ccagcgagcc gaggttggcg gaaacgaagg ccaggccggc gacccatgcg gtcatttcgc
      781 ggccggccag gaagaagtct tcgccagttt tggcgaagcg cttcagataa aagccgatgc
      841 ccaggaccag ggcgaagtaa aagaccacaa tgcccaggtc taccggggac atcttgacca
      901 gacgttcgct cgccagcatt cccaagatcg ccgtcatctg tgccttcctg tgttcctagc
      961 tgctgcgtgc gaaatcaccg agacgccctc accgggtcac cgcgccaact aaaccgttgc
     1021 gccaggcgtg accagcagcg ggaagcgcgc cgctataatt tacgaaacgc ggacagcttg
     1081 caacgcttaa acggcctggt gtttcgtaaa aaaaggggag gcaattcctg ccaccccgca
     1141 agcaaaatcc aaagctgcaa acgcgacgtt gccccgcaca tcacaacaaa gcagagctca
     1201 tctccgaaaa actggggtag gagaaaaagc cccggggtcg gtaaaaagcc ggttgcagcg
     1261 gtccggcttc ctgcgggaaa ggaagcgagc cggcgaacga agaaagaaag ggcttcaacg
     1321 ttaccgtaaa cgcttacgat ccgatggtac acgtttacac tggctgggga cgggttgtca
     1381 agggtgcggc ccgaggggtt acgcggtcag atgacggcga ccgagcagct agggacaccc
     1441 gctgccgccc gctccacgcc ggcgttttat ttggcggaga atttgtagat ggtctcggag
     1501 tgatagtcct ggccaggacg caccacggtc gatggaaagt ctgggtgatt cggcgaatcg
     1561 ggaaagtgct gcgtttccag gcacagcgcg ccgaacttcg catacgcgcc gccgatgcct
     1621 ttgagcgagc cgttgagccc gaggcttaca taaagctgaa cgcccggctc ggtggtccag
     1681 atttccattg cgcggccgga ggcgggatcg gaaacggagc cgatcttgcg cagcttgccg
     1741 gaggtcccgt ttaaaacaaa gttgtggtca tagccgccgg tgccgggcac ctgggcgttg
     1801 cgctcgccga tggcggtggg cttgcgaaaa tcaaatggtg tgccttccac cgacttgagt
     1861 tccccggtgg gaattaatgt ggcatcgacg ggagtgaagc ggtcggcgtc gagctggagg
     1921 acttcgttga gaatgtcgcc gctgccggct ccggaaagat tgaagtagct gtgattggtc
     1981 agattgacga ccgtgggctt gtcggtcgaa gcgtgatagg taagtttcaa tgcgttgtca
     2041 tcggtcagtt cgtaggtcac aaccgtggtc agtgtgccgg gatagccttc ttcgccgtct
     2101 ttgctgacgt aggtaaattt caccgcggcg ccgtgattgg ctttcactag ctcggctgtc
     2161 cacactttgc gattaaaccc ttccttgccg ccgtgcagag aattcggccc gttgttcagg
     2221 aatagcgaat attcgtggcc atcaatggaa aatttgcctt tggcgatgcg gttgccgtag
     2281 cggccgatcg tcgcgccgaa aaagggatgg tcgccggtat atgcggccaa gctgtcgaag
     2341 ccgagaacga catcagcgtt tttgccggct ttgtcgggca cccggagttc cgtcagcgtt
     2401 gcgccgtagg tgatgacttt ggccccggcg cccttggcgc tgtgcagcgt gtactcctcg
     2461 atttcagtgc cgtcaggaag tttgccgaac gatttctttt ccactttttg tgctccctcg
     2521 ggaggatttg cctccgcgcg cagatcggtg ggaatcacca tgagcgcgag aaatgggaaa
     2581 gctgccaggc agcatattgc gatggtgcgg ccgttcatgt tcgacctcca caaaaaaccc
     2641 tcgaaagggt actacgattc gtccacgctc tgggaagcca gcttcagcgg aacccgcgtt
     2701 gcatcggttt cacctgtcgc tgccagagag attttcgctg agcgttactt ctaccatgcg
     2761 tccgtcgtat ggaatcactt ccctcggcgc gaacttggct tcctcgaagc cgattcccgt
     2821 tcccttgcgg acaaacacga cgactaacgg aagcgtttgc ggcattccgg catacgagcc
     2881 tttgcgcgcc ccaattatca gtcggtgcgt cgcctcattc caatgaatcg gaatcgttgc
     2941 aaaggcgccc ttctcatagt catacgtgtc gccgtcgtct tcgtagaacg tgaaatcgcc
     3001 gtcggcgcct gtgtagacgc ggatctccaa gggatcgttg gatggttgct gcgcatattc
     3061 gatggtaggc cccataggca ggatcgctcc cgctctcacg aaaatgggca tctcttccag
     3121 cggagcgggc gtatcaatcg cgcggccgcc ttcgacggat tcgccggtcc aaaagttgta
     3181 ccagttgcct tttggcagat agaggtggcg cgtggtggcg ccgggctcgg tgactggatt
     3241 caccagcagc gagggcccga acaaatactg gtcgccgatg ttcagaacgc ggggatcgtc
     3301 gcggaaatcc atcgccagcg cgcgcatctg ggtgtagttc tcgctggtgg ttttccacgc
     3361 gacagaatag atgtatggga gcaggcggta acgcaagcgg tcatagcgct ccagaatcgc
     3421 ctgcatttgc gggccgtacg accagatttc attttgattt gtagtgcgcg tgccatgcgc
     3481 gcg
//
LOCUS       DASTPM010000202         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_381909, whole genome shotgun sequence.
ACCESSION   DASTPM010000202 DASTPM010000000
VERSION     DASTPM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381909"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1018..1782
                     /locus_tag="VFR42_03540"
     CDS             1018..1782
                     /locus_tag="VFR42_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368257.1"
                     /translation="MQKTNGIASQVSVLVAESCPMNCELMSSALERFKQRLTVVASAT
                     DSGEIQAAFEQHHPDVCIISAQLKDGPTAGFAALRELIAKFTEARAILLLDASERHAV
                     VDAFRAGAQGVFSRAQPLEILCKSVHKVHEGQVWASNDQIRYLIDALAAPPPPITDAK
                     GAPLLTKREQTLVQLVTEGRSNKDISRELNLSEHTVRNYLFRIFNKLGTSSRLELALY
                     AINQRQAQHQSQPEVQRDSQPEPEPPDRTNSKATAA"
     gene            complement(1779..3884)
                     /gene="mgtE"
                     /locus_tag="VFR42_03545"
     CDS             complement(1779..3884)
                     /gene="mgtE"
                     /locus_tag="VFR42_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0368258.1"
                     /translation="MSHKTRRGRLKIYLGYAAGVGKTYRMLEDAHEMQGRGMDVVLGF
                     LEPQGRSDVNEKAREFESIPLLRVACRGSHTEELDVVGILRRAPKVCIIDDLAHTNAP
                     GSERQRRWQDVQALLDGGMDVLTTMNVQDLASLSDQILHITGLRVRETVPDWVFQEAD
                     EVVMVDVTPRALLHRLERGAIYTPERAKVESERLFQEPTLVALRELAIRQTAQALEAR
                     AAAKRREQTEKGPEKILVNVTADPSTAMLLRRARRVADYLHAGCVAVFVCAAEDFSKL
                     PVPERQAVERHLRFAQDLRVETAIINGNNRARVLVDYAHNNGVTQVFIGPAAESQKRW
                     FRGLDFTEQVLYQARDLEVTVVAERSREGGRPSLYPEDEAAGLMHSGFVRISEEMTVE
                     EAIATIRRQAENVEMIYYAYVLDDERHLQGVMSFRELVSADRSKKVRDTMRKDFVFVP
                     EDEDKEVVAQILAKHRLLAVPVLDMNRHMLGIVSSADLAGVVQQAASEDILKIGGMEA
                     LEGPYMDVTFLQMIKKRAGWLAVLFLGEMLTATAMGYFSDEISRAVVLALFIPLIISS
                     GGNSGSQATTLVIRAMALGEIRLRDWFRVIRRELMAGLALGAILGMIGITRIFLWHLL
                     RGTYGEHYGIIALTIGCSLIGVVMFGTLAGSMLPFILRRCGLDPASASAPFVATLVDV
                     TGLIIYFSVASVILRGVML"
     gene            complement(3921..>4876)
                     /locus_tag="VFR42_03550"
     CDS             complement(3921..>4876)
                     /locus_tag="VFR42_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0368259.1"
                     /translation="LGYASGVGKSFRMFDEGRRRKERGQDVVVGALQSKIPPDIEPLL
                     STLEIVPTVSIKGVPVMDVEAILRRQPHICLVDGLAYDNPPGSRNAHRWQDVEELLHA
                     GITVVGSVNLQYIEDQRDAVEALTGKRVTQTIPREFLNTADEIVIVDAPTEKGTERRT
                     ALREMALVLSADVIDAGLQRYLRSHGIEQTWGMHERFLICLTPRANFERMIASARRSA
                     ARFHCDVLAVYVKQEKLSGADQKSLEEGLAKARAGGLRVEMLEGEDPVATIVQYARAH
                     GVSQIFVGHSAKKDLGTRMFGSPVSRLVRAARGMDVRVFPH"
BASE COUNT          955 a         1685 c         1156 g         1080 t
ORIGIN      
        1 gatcgagcgt cttcttgtaa aacgggctgg acctgcgggc tccggttctg ctcttcgttc
       61 ctaccctgcg aagaaccagg caaactgctt ctggcgccca actcttccct gcgagcgctc
      121 ttccactctt ttcgggttgc ttttgattcc gtgtcgcttg gccttctttg cgtgtcatcc
      181 gttccgctcg ttactcgatc accttcgaac actgcaggct cattctcatc cgccagttgg
      241 ttcggggatc cggtctgcaa ccgctagaaa ctactctccc tcacgctact ggatcacccc
      301 taagcctctc ctcacgttcg ttctcgccgg ctcttttcta tcttaattac accatgcata
      361 gttccctgcg ccctgcttgc tccgaaatca gcttaggttc tctttcgtct gccgcaaaga
      421 aacgcaatat ctggtgacaa aaggagttac ggaccatcgt gatttgtggc aattcatcct
      481 cgggaatgac ctgtaccttt cggtctatgc gcaaacattt cgctctcctg gctgaacggc
      541 tgtgctcaaa tcgctggtga caaaactctc cggtcgctct tctcgtttgg cagcccgcat
      601 gcactcacct aaatgcatga ccattactct cgacagccta gaaacgcttc tcttcgcgat
      661 aacacgcaac cagacacgaa tttacggggg actagtacac cattcactgc atacgcagaa
      721 attccattgc agaacctttt acaccattga atgaaaaaaa gagttacacc gaaaaaggga
      781 ctttgcgatt tcagcatcgc ttcctttatc gcaatccggt tagttctggg atcacttgct
      841 agggaggatt gatttcttga agtactctta gaaacatact tagaacttcg ttacgctatc
      901 ggccctgagc cttccggccc tgttcgactt cgcccccagt cgttgcccag ttctcgcaac
      961 gcccagcccc aagcccagct taagccctgg tccacaattt cacgcggaag gaccgctatg
     1021 caaaaaacga acggcatcgc ctcgcaggtt tctgtcttgg ttgctgagtc ttgtcctatg
     1081 aattgcgaat tgatgagttc cgctctggaa cgattcaagc agcgcttaac tgtggtcgcc
     1141 tcagccaccg attccggcga aattcaagcc gccttcgaac agcatcaccc tgacgtctgc
     1201 atcatcagcg cccagctcaa agacggcccc accgccggct tcgcggccct ccgtgaactc
     1261 attgcaaaat ttacggaagc tcgcgcgatt cttctcttgg acgcttccga gcgtcacgcc
     1321 gtggtggacg cattccgagc cggcgcccaa ggtgtgttct ctcgcgcaca gcccctcgaa
     1381 attctctgca agagcgtgca caaagttcac gaaggccagg tttgggccag caacgaccaa
     1441 atccgctatc ttattgacgc gctcgcggct ccgccgccgc ccatcaccga cgccaagggc
     1501 gctcccctgc tcaccaagcg cgagcaaact ctggttcagc tggtcaccga aggacgctcc
     1561 aacaaggaca tttcgcgcga actcaatctg agtgagcaca ccgtgcggaa ttatctgttt
     1621 cgcattttta acaagctcgg cacgtcttct cgcctcgagc ttgcccttta cgccatcaat
     1681 cagcgccaag ctcagcacca atcccagccg gaagttcaac gggactctca gcccgaaccg
     1741 gagccgcctg accggaccaa cagcaaggcc accgccgcct aaagcatcac tccgcgcagg
     1801 attacgctgg ccacgctgaa ataaatgatc agtcccgtca cgtcgaccag cgtggccaca
     1861 aacggagccg acgcgctcgc cggatccagc ccgcagcgcc gcaggataaa cggcagcatc
     1921 gaacccgcca gcgttccgaa catcaccacg ccgatcaggc tgcatccgat agtcagcgcg
     1981 ataattccat agtgctcgcc atacgtaccg cgcagcaggt gccacagaaa aattcgcgtg
     2041 atgccgatca ttcccaggat tgcgcccagc gccaacccgg ccatcagctc gcgtcggatc
     2101 actcggaacc agtcgcgcag ccggatttcc cccagcgcca tcgctcgaat caccagcgtc
     2161 gtcgcttgcg aacccgaatt tccgccgctg ctgatgatca gcggaatgaa cagcgccagc
     2221 accaccgcgc gcgaaatttc atccgaaaaa tatcccatcg cggtggcggt cagcatttcc
     2281 cccaagaaca gaaccgccag ccagcccgct ctcttcttga tcatctgcag aaaggtcacg
     2341 tccatgtagg ggccttccag cgcttccata ccgccgatct tcaggatgtc ttcgctggcg
     2401 gcttgctgca ccactcccgc caagtccgcg ctactcacaa ttcccagcat gtgccggttc
     2461 atatccagta ctggcacggc cagcaatcgg tgttttgcca atatctgggc tacgacctct
     2521 ttgtcttcgt cctccggcac aaaaacgaaa tctttcctca tggtgtcgcg caccttcttg
     2581 gagcggtccg ccgacaccag ctcgcggaac gacatcaccc cttgcaggtg ccgctcgtcg
     2641 tccagaacgt acgcgtagta aatcatctcc acgttttcag cctgtcgccg aatcgttgcg
     2701 atcgcttcct cgaccgtcat ctcctcggaa atccgtacaa accccgagtg catcagccct
     2761 gccgcttcgt cttccgggta gagcgagggc cgcccgcctt cccggctccg ctctgccacc
     2821 acggtcactt ccaggtctcg cgcctgatac agcacctgtt cggtgaagtc cagcccccgg
     2881 aaccatcgct tctgcgattc cgccgctggc ccgataaaga cttgcgtcac tccgttgttg
     2941 tgcgcatagt caaccagaac tcgggcccgg ttgttgccat tgataatcgc tgtttccact
     3001 cgcaagtctt gtgcgaaacg caggtgccgc tccacggcct ggcgttccgg cacaggtagc
     3061 ttgctgaaat cctccgccgc gcagacaaac acagccacgc aacccgcgtg cagataatct
     3121 gccacccggc gggccctccg cagcagcatc gcggtggaag gatcggccgt cacgttcacc
     3181 aggattttct ccgggccctt ttcggtttgt tcccttctct tcgccgcggc ccgcgcttcc
     3241 agcgcctgcg cggtttgccg gatcgccaat tcgcgcagtg ccaccagcgt cggctcctga
     3301 aacagccgtt cgctttccac tttggcgcgc tccggcgtat aaatcgcgcc gcgctccagc
     3361 cggtgcagca gcgcccgcgg cgttacgtcg accatcacca cttcgtcggc ttcctgaaac
     3421 acccagtccg gtaccgtctc tcgtacccgc aacccggtga tgtgcagaat ttgatcgctc
     3481 agactcgcca gatcctgcac gttcatcgtc gtcaggacgt ccatcccgcc gtccagcagt
     3541 gcttgtacat cctgccaacg ccgttgccgc tccgaccctg gcgcattggt gtgcgccaaa
     3601 tcatcgatga tacaaacctt cggcgcccgc ctcaggatcc ccacaacgtc aagttcttcg
     3661 gtgtgactgc cgcggcaggc cacccgcagc agcggaatcg attcaaactc tctcgccttc
     3721 tcattgacgt cacttcgccc ctgcggctcc agaaagccca gcactacgtc cattccgcgc
     3781 ccctgcatct cgtgcgcgtc ttccagcatg cggtaggttt ttccaacgcc cgccgcatat
     3841 cccaggtaaa tcttcaaacg gccccgtcgc gttttgtgac tcattgaatt ttctctcgca
     3901 tgaaaaaacc ggaaagcaaa ctagtgggga aacactcgca cgtccatccc tcgcgccgcc
     3961 cgcaccagcc ggctcacggg gctgccaaac attcgcgtgc ctaaatcttt tttcgcgctg
     4021 tgcccgacaa atatctggct gactccatgc gcgcgcgcat actgcacgat ggtcgctacc
     4081 gggtcctcgc cttccagcat ttccacccgc agcccccccg ctcgcgcttt cgccagccct
     4141 tcttccaggc tcttttggtc ggctccggaa agtttctcct gcttcacata cacggccagc
     4201 acatcgcaat gaaaccgcgc cgcgctccgc cgcgcgctcg cgatcattct ttcgaaattc
     4261 gcccgcggcg tcaggcaaat tagaaacctt tcgtgcatcc cccaggtttg ttcgatccca
     4321 tgcgagcgca aataacgctg taatcccgca tcgattacat cggcggaaag aaccagtgcc
     4381 atctctcgca gcgccgtccg ccgttccgtt cccttctcag tcggcgcatc cacaatcacg
     4441 atctcgtccg cagtgttcag aaactcgcgc ggaatcgttt gcgtaactct ttttcccgtc
     4501 agcgcctcca ccgcgtcgcg ttggtcttcg atgtattgca ggttcaccga tcccaccacg
     4561 gtgatgcctg cgtgcagcag ctcttctacg tcctgccagc ggtgcgcgtt tcggctaccc
     4621 ggcggattgt cgtacgccaa tccatccacc aggcaaatgt gcggctgcct gcgcaggatc
     4681 gcttccacat ccatcaccgg cacgcccttg atgctgaccg tcggaacgat ctccaacgtg
     4741 ctgaggagcg gttcgatatc cgggggaatc ttgctttgca gcgctccgac cactacgtcc
     4801 tggccgcgtt cctttcgccg cctgccttca tcgaacatgc gaaaggactt gccgacgcct
     4861 gacgcgtagc ccagga
//
LOCUS       DASTPM010000203         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1333103, whole genome shotgun sequence.
ACCESSION   DASTPM010000203 DASTPM010000000
VERSION     DASTPM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333103"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1466
                     /locus_tag="VFR42_03555"
     CDS             147..1466
                     /locus_tag="VFR42_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0368260.1"
                     /translation="MRKLFVMIAVCGLIAMPASSQEKKSGKSYVLKAARMFDGKSESL
                     ATPGMVVVQDGKIVGVGANANAPAGAEVIDLGDATLLPGFIDAHTHLTMMYREDYTKA
                     MLDSLQKPIPEMALDASVNARVTLMAGFTTVRDVGSSHYLDAGLRNAINRGVVPGPRM
                     LVAVHAIGATGGHCDETGFREGALGKESGPEDGVVNGADEARRAVRLAHKYGATVIKT
                     CATGGVLSLADAVDTPQLTQAELDALVDEAHALGLKTAAHAHGAEGAKRAIRAGIDSI
                     EHGSFLDDEDLNLMKQKGTYYVPTLMAVQGLTEQIQKGVAIPPSIRPKAEAAIAAIHQ
                     TFQRAIAKGVKVGLGTDAAVYPHGRNAEEFHQMVDLGMKPIAALKAGTSADADLLGLA
                     DKIGTLESGKLADVVAVPGNPLENIRETEHVFFVMKEGKIFKNEKPN"
     gene            1513..>2125
                     /locus_tag="VFR42_03560"
     CDS             1513..>2125
                     /locus_tag="VFR42_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368261.1"
                     /translation="MSNLRDSGFRHRPTIAPREWAGAAGLSLLVAVAVVSPFFFLGTA
                     SGHDISFHMASWLDAAGQWKQGLLLPRWTEWANYGYGEPRFIFYPPLSWLLGACLGSV
                     IPWRAVAATFVVGVQTFAGISAFALLRRLVDSRWAALFGAACFAANPYALVIIYARSD
                     FAELLAMAFFPLLFGSALRLCGFLEEQTNRSDRLQGIFFFAVAF"
BASE COUNT          446 a          505 c          732 g          442 t
ORIGIN      
        1 gaaggcgcga agttcaggac aaccgagcac gcgcaacgac gcctacgggg cagcgaggca
       61 gcgaccggga gcttgacatt tgcgagcagc acgctatttt gggcgttcgg attttggcct
      121 gcaaactctg gagatggaga acaacgatgc gcaagctttt cgtaatgata gcagtgtgcg
      181 gattgatcgc aatgcccgca agcagccagg agaaaaaaag cgggaagagc tacgtattga
      241 aggccgcgag gatgtttgac gggaaaagcg aatcgttggc gacgccggga atggtggtgg
      301 tgcaggacgg aaaaattgtg ggagtcggcg cgaatgcgaa tgctcccgcg ggcgccgagg
      361 tgattgatct gggcgatgcg acgctgctgc cgggatttat cgatgcgcac acgcacctga
      421 ccatgatgta tcgcgaggat tacacgaagg cgatgctgga ttcgctgcag aagccgattc
      481 cagaaatggc tctggatgcg agcgtgaacg cgcgggtgac actgatggcc ggattcacga
      541 cggtgcgcga cgtcgggtcg tcacattatc tggacgcggg cttgcgcaat gcgatcaatc
      601 gaggcgtggt gccggggccg cggatgctgg tggcggtgca cgcgatcggg gcgacaggcg
      661 ggcactgcga tgaaacgggt tttcgcgaag gggcactggg taaagagtcg ggaccggaag
      721 acggcgtggt gaatggagcg gacgaagcgc ggcgcgcagt gcgtttggcg cacaagtatg
      781 gtgcgacggt gatcaagact tgcgcgacag gaggtgtgtt atccctcgcc gacgcagtgg
      841 atacgccgca gctgacgcaa gccgaactcg acgcgctggt ggatgaagct cacgcactag
      901 ggttgaagac ggcggcgcac gcgcacgggg ccgagggagc taagcgggca attcgcgcgg
      961 ggattgattc catcgagcat ggatcgtttc tggacgacga ggatttgaat ctgatgaagc
     1021 agaagggcac gtattacgtg ccgactctga tggcagtgca gggactgacg gaacagattc
     1081 agaagggcgt ggcgattccg ccatcaatac ggccgaaagc ggaggcggcg atcgcggcga
     1141 ttcaccaaac atttcagcga gcgattgcga agggcgtgaa ggttgggctg ggcacggatg
     1201 ccgccgtcta tccgcacgga cgaaatgccg aggagttcca tcaaatggtg gacttgggga
     1261 tgaaaccaat cgcggctctg aaggcaggaa catccgcgga tgcggatttg cttggactcg
     1321 ccgataaaat cgggaccttg gaaagcggca aactggcgga cgtggtggcg gtgccgggaa
     1381 atccgctgga aaacatccgg gagaccgaac atgtattttt tgtaatgaag gaaggcaaga
     1441 tcttcaagaa cgaaaagcct aattgacgct tcgcaggagg attgtggttc cggttacaat
     1501 tccctgcact caatgagcaa cctgcgtgat tccggattcc ggcaccgtcc cacgattgcc
     1561 ccgcgagaat gggcgggcgc ggctgggctg tcgttgctcg ttgccgttgc agtcgtaagt
     1621 ccattctttt ttctgggtac agcttccgga cacgacatct cgtttcatat ggcttcgtgg
     1681 ctggatgccg cggggcagtg gaaacaggga ctcttgctgc cgcgatggac tgagtgggcc
     1741 aattacgggt atggcgaacc gcggtttatt ttttatccgc cgctttcctg gctactggga
     1801 gcctgccttg ggagcgtaat cccgtggaga gcggtggcgg ccacgtttgt tgttggcgtg
     1861 caaacatttg cgggcatatc tgcgttcgca ttgttgcgac ggctggtgga ttcgcgttgg
     1921 gcggctctgt ttggagcagc atgcttcgcc gcgaatccgt acgcactggt catcatttat
     1981 gcgcgaagtg attttgcaga actgctggcg atggcatttt ttccgctgct ctttgggagc
     2041 gcgcttcgct tatgtggctt ccttgaggaa cagacaaacc gttcagaccg gctccagggt
     2101 atattttttt ttgcggttgc gtttg
//
LOCUS       DASTPM010000204         9076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_814313, whole genome shotgun sequence.
ACCESSION   DASTPM010000204 DASTPM010000000
VERSION     DASTPM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9076
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814313"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VFR42_03565"
     CDS             <1..1403
                     /locus_tag="VFR42_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEU0368262.1"
                     /translation="ITICMNVPPARAQKLAAGIEVVQSSEEKHESLAEKPSLTFHAER
                     TPALTIFVDDTVKYQEIDGFGASLTESSAWLLKRKLTDAQRSDAVRMLFDRQKGIGLS
                     MLRQPMGASDFALQEYSYDEMPAGETDPELKKFSIDHDRADILPVLKEILAVNPKIKV
                     IGSPWSAPGWMKTSGSMTQGALLPSAYDAYANYFVKYVQAYGAEGIPIFALTMQNEPL
                     HVPHDYPGLGMMAREQTAFLRDHLGPAFQKAGVNAKILIFDHNWDLIDYPINVLNDSN
                     AARFAGGIATHCYGGSATAQDELHQRFPGQEIWMTECSGGDWQKGKVLQQQVQLIINV
                     TRHWAKSVILWNLALNQNHEPYLGGCTTCRGEIVVDESSSPAQVKTTADYTALGHASK
                     FVRPGARRIDSNTFGQGSLEDVAFQNPDGSIVLLVLNSGEGTAQFNIGWRGQFASYKL
                     ASGTAATFVWNGTTAK"
     gene            complement(1418..2881)
                     /locus_tag="VFR42_03570"
     CDS             complement(1418..2881)
                     /locus_tag="VFR42_03570"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEU0368263.1"
                     /translation="MLLSTRLVIFGVGVALLLILLWPLLHAAPDGLEHGEVSQFVGRF
                     HPLIVHLPIAFLLLVPLLEGAGWIPRWSRVREAAEFVLMLATVSVFVAAFLGWLLARS
                     GGYEGKLVTSHMWGGFTLAAATLVCFILRAWDKRAYGAALSATLLVLFWTSDKGGQLT
                     HGETFMTKHMPSELRSVFHMPPAANTTQHAAEVTKVPPTDRVPASVAAFPLPSATFFD
                     ARVAPIFENKCSSCHNAQKKKGKLRLDSFENVMHGGKDGVVIKAGDPRHSELFRRINL
                     PPDDKDFMPTDGKPPLTASEIKVIQLWIASGASSSLAANEVPGAPPLPASEQPALPLA
                     KDYRPQLPLITSLERELGVHLVPRSRNPEDGLILRTASAPERCDDAALARLKAIGNLI
                     VDAELARTRITDIGLKTLVTFPNLRVIDLAHTAVTSAGVPTLARLNRLQSLNLTATAV
                     DDRGVAALRRKQTLKRLYLFETKCTTRSALGSREEVP"
     gene            complement(2891..3946)
                     /locus_tag="VFR42_03575"
     CDS             complement(2891..3946)
                     /locus_tag="VFR42_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU0368264.1"
                     /translation="MRSRSRRSFLARCSAAAAAAAIPPFYLHTSDKSGQRLPTVGSGP
                     HTYECVHDWLIPPDGLVWGDTHGLAQDEQGRIYVAHTVNKSSMRGEAIVVYGADGRFV
                     SAFGEEFRGGAHGLSIRRETDGEFLYHCDINRCKIVKTTLDGKTVWSHAYPREDGAYA
                     ERPIDFVPTNVAFAPNGDFYVGDGYGSHHVLRFSQGGKFLGEIGRPGHGDGAFDTPHG
                     LWVDPRGKEPVLVVADRGNRRMQTFSLEGAHIRTVKDEAHLRMPCNFHVQGEWMVCPD
                     LDSQVCIFDSEYNVIAQLGDGQAANGEVGSRRKQTRREFTPGQFITPHAAIFLRSGDI
                     LVAEWLPIGRITLLRRV"
     gene            complement(3943..5502)
                     /locus_tag="VFR42_03580"
     CDS             complement(3943..5502)
                     /locus_tag="VFR42_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0368265.1"
                     /translation="MKAKNHEQSGHCCDHGGAGPTVYDLPIPLELREEWRTLATRRHF
                     LGKMGKTLGWASLATLFGNVIAARVARASADSQPGSPSHYVPDFAPKAKRVIYLFMSG
                     APPQMDLWDYKPGLAGLYDKDLPDSVRGTQALTGMTSGQARFPIAPSHWGFTRQGKSG
                     RWVSDLLPWTGRLVDQLTLIQSLETDAINHEPAILLMNTGNMVPGKPSMGSWLSYGLG
                     SMNENLPTFVVLNSLTLPGTDLQPISPKLWSSGFLSNEYAGVAFRSQGEPVLYLGDPQ
                     GMGRETRRTLIDAVNSINQMTFEEVGDPETHARIHQYEMAFRMQVSLPELTDMRDEPD
                     STWTLYGDEAKQAGTFAYNCLLARRMAERGVRFTQIYQRGWDVHSNAVGNLPKLCAAT
                     DRACYALVTDLRRRGLLDDTLVLWGGEFGRTVYSQGGLSKENYGRDHHPRCFTMWMAG
                     GGTKAGLVHGQTDDFCYNVVKDPVHVRDFNATVLRLLGIDHEKLKFKFQGLEQKLTGV
                     VPAKVVPELIA"
     gene            complement(5499..8783)
                     /locus_tag="VFR42_03585"
     CDS             complement(5499..8783)
                     /locus_tag="VFR42_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1553 domain-containing protein"
                     /protein_id="HEU0368266.1"
                     /translation="MVLSQRGRRSGSPLYGTALYVLAGWLCLNPCAAAQSREAKPAAV
                     PENPQKLSFNGTIQPILSENCYACHGPDPGGRKAGLRLDRSEFSLAPHQDSHEKYGPA
                     ILPGKPDKSPLVQRIEAQNAKERMPPPEAHKTLKPDQIALLREWVKQGAQYEELWSFL
                     PPKQQAVPVVQNSESVRNAIDNFVLAQLEKEGLKPSPEADRRSLIRRVTYDLTGLPPT
                     PEEVQGFVADSAPDAYEKVVDRLLASPRYGEHRAHYWLDVARYGDTHGLHLDNFRSIW
                     AYRDYVIRSYNENKPFDQFVREQLAGDMLPVKTLDSLTASAYLRAGISSGEGGTLIEE
                     LRVNNKRERTEAYGAAFLGLTVGCANCHDHKFDPITQKDFYRLTAFFNNLTEIPSNDD
                     RNDWPPFLRLPQAENRDSYEQTLAKRSELERRMAVHREQAHELIGAWIGQRTSAAVPV
                     SSEGLAVRLRFDEQNGSTFADSAPGASHKHVSATGAPVVWGEGTWFWPYMRMEISTKL
                     ELPSAGEVEANQPFSAATWVRPHLRSLEAKDEEKPAGVILSAADSGQKQRGWQLRVSK
                     GKLEFVLANEMPDNAIQVATKLKVLTVGRWNHVLATYDGSGKAAGVVLYIDGRAQELA
                     VVKDSLQGSTRTSAPVEFGRMYPDSDPLRQTAFQDFRFFNRALSTSEASRLPVEDYVS
                     EIVRKPMSSWSEDEFHTVSEFYFAQRDDVTQKLRAQIAPLNEELERLSKDGAITLVSE
                     EGPGLPYADVLTRGAFGARTERVRPGVPHFLPPLPANAPLNRLGLAEWTVSASNPLTA
                     RVTVNRMWAEIFGAGLVETTEDFGVMGGRPSHPELLDWLALDFRANGWEVKRFYRQLV
                     LSATYRQSAQVTPQLLENDPKNRLLARGPRYRMDGEMLRDTALATSGLLVEKIGGPSV
                     KPYQPAGVWEAGSHQGSDTKSYTQDHGDSLYRRSLYTFWKRMATMPDMDTLDVPVRDA
                     ACTRRQRTDTPLQALVLMNDPQWLEASRRLAERVIRHSSDTSTRLDYLGEVLLARDWQ
                     PREKSVLENALHKFQSTYEHDQVRAEEILKVGESSRDTGIRSDELASWMLVTSAAMNL
                     DAVLNK"
BASE COUNT         1892 a         2692 c         2550 g         1942 t
ORIGIN      
        1 gcatcacaat atgcatgaac gtcccgcctg cccgagccca aaaattagcc gccgggatcg
       61 aggtggtcca gtcctccgag gaaaaacacg aatcgctagc cgaaaaaccg tccttgacct
      121 tccacgccga gcgcacgcca gcgctaacca ttttcgtgga cgacaccgtc aaataccagg
      181 agatcgacgg attcggagcg tcattgacgg agtcttcggc atggttgctg aaacgtaagt
      241 tgacggacgc acagaggagc gacgcggtgc gcatgttgtt cgatcggcaa aaaggaatcg
      301 gcttgagcat gttgcgccag cctatgggcg ccagtgattt cgcgctgcag gaatacagct
      361 atgacgaaat gccggccggc gaaaccgatc ccgaattgaa gaaattctcg atcgaccacg
      421 atcgcgccga catcctgccc gtgctgaaag aaattctggc ggtgaaccca aagatcaagg
      481 ttattggatc gccttggagc gctcccggct ggatgaagac gtcaggctcg atgacgcagg
      541 gcgcgctttt gccttccgct tacgacgctt acgcaaacta ttttgtgaag tatgtgcagg
      601 catacggggc cgaaggcatt ccgatttttg cccttaccat gcagaacgaa ccgcttcatg
      661 tgccgcacga ctatcccggc ctgggaatga tggctcgtga gcagaccgca ttcttgcggg
      721 accatctcgg cccggcgttc caaaaagcgg gtgtaaacgc aaagattcta attttcgatc
      781 acaactggga cctcatcgac tatccgatca acgtcctgaa cgattcaaac gccgcccgct
      841 ttgccggtgg catcgccacg cattgctatg gcggcagcgc caccgcgcaa gacgaattgc
      901 accagcgctt tccgggccag gaaatctgga tgaccgaatg ttccggcggc gactggcaga
      961 aaggcaaagt cctgcagcag caggtccaac tgatcatcaa tgtgacccgg cactgggcca
     1021 aaagcgtgat cctgtggaat ctcgcgctga accagaatca tgagccgtac ctgggcggct
     1081 gcaccacgtg ccgtggcgaa attgttgtgg acgaatcgag ttcgccagcg caagtgaaaa
     1141 caacagcgga ttacacggcg ctcggccacg ccagcaagtt tgtacggccc ggcgcgcgtc
     1201 ggattgattc gaacaccttt gggcaaggca gcctggaaga tgtggcattc caaaacccgg
     1261 atgggtccat cgtgctgctg gttttaaatt ccggcgaagg aaccgcgcaa ttcaacatcg
     1321 gctggcgcgg acagtttgcc agctacaagc ttgccagcgg cactgcggcg acgtttgtgt
     1381 ggaacggcac tacggcgaaa taaagcgttg cacatcgcta cggcacctcc tctcttgatc
     1441 ccagcgcaga ccgagtcgtg cattttgttt cgaaaagata cagccgcttg agggtttgct
     1501 tgcggcgaag tgcggcaacc cctcgatcgt caaccgcggt cgcggtcaga ttcagcgact
     1561 gcaaacgatt gaggcgagca agagtcggca cgcccgccga agtaacggcc gtgtgcgcaa
     1621 ggtcgatgac acgaaggttg gggaacgtca caagtgtttt taggccaatg tcagtaattc
     1681 ttgtgcgagc cagttccgcg tctacgatca gatttccaat agcttttagc cgggcgagtg
     1741 cagcgtcatc gcagcgctcc ggcgcactcg cggtacgcaa gattaatccg tcttcgggat
     1801 ttcgcgaacg gggaacgagg tgcacaccca gctcgcgctc gagcgacgtg attaacggaa
     1861 gttggggccg ataatctttg gccaagggca gcgcgggctg ctcggaagcc ggaagaggcg
     1921 gagcgccggg cacttcattc gcggccaggc tgctcgatgc ccccgacgcg atccaaagct
     1981 gaattacttt gatttcggag gctgtcagcg gcggcttccc gtcggtgggc atgaaatctt
     2041 tatcgtccgg cggcaaatta atccgccgga acaattcgct gtggcgcggg tctccggctt
     2101 ttataaccac gccgtctttt ccgccgtgca tcacattttc aaaactatcc agccggagtt
     2161 tccccttttt cttttgtgcg ttgtggcagc tggagcattt gttttcaaag atgggagcta
     2221 cccgtgcgtc aaaaaatgtt gcggatggca aaggaaaggc ggctacactg gccgggactc
     2281 ggtcggtcgg cggtactttc gttacttccg cggcatgctg cgttgtgttt gctgccgggg
     2341 gcatgtggaa cacgctccgc agttcgcttg gcatgtgctt ggtcatgaac gtttcgccgt
     2401 gggtcagctg gccgcctttg tcgctcgtcc agaacagaac cagcaatgtt gcagaaagcg
     2461 ccgccccgta ggctctcttg tcccaggccc gcaggataaa acagaccagc gtcgcggccg
     2521 ccagcgtgaa accgccccac atgtggctgg ttaccagttt tccttcgtat cctccgctcc
     2581 gggccaaaag ccaacctaga aatgccgcca cgaagacgct aactgttgcg agcatcagaa
     2641 cgaactccgc cgcctctcgc acgcgactcc agcggggaat ccagcccgcc ccttccagta
     2701 gcggaaccag cagcaagaac gcgatgggga gatgaacgat tagaggatgg aagcggccca
     2761 caaattggct tacttcaccg tgttcgaggc cgtcaggcgc ggcatgaagc agaggccaga
     2821 gaagaattag aagcaaagcg acgccgacgc caaatattac caaccgagtg gacaaaagca
     2881 tcgagcggcc tcagacgcgc cgcaagagcg taattcgccc gatcggcaac cactccgcca
     2941 ccagaatgtc gccgcttcgc agaaaaatgg cggcgtgcgg cgtaatgaac tgccccggcg
     3001 taaattcacg cctcgtctgc ttccggcgag aaccaacttc tccatttgcg gcttggccat
     3061 cgcccagttg ggcgatcacg ttgtactcgc tgtcgaatat gcagacctgg ctgtcgagat
     3121 ccggacagac catccactct ccctgaacgt gaaaattgca gggcatgcgt aaatgggctt
     3181 catccttcac tgtgcggatg tgagcacctt cgagcgagaa cgtttgcatg cgccggtttc
     3241 cgcgatcagc caccaccagc acgggctctt tgcctcgtgg gtccacccac aaaccgtgcg
     3301 gtgtatcgaa cgctccgtcg ccgtggcccg gccggccaat ttcgccgaga aatttaccgc
     3361 cttgcgaaaa gcgcaacacg tgatgcgagc cgtacccatc gcccacatag aaatcgccat
     3421 ttggagcgaa ggcgacattt gtagggacaa agtcgatcgg ccgctcggca taggcgccat
     3481 cttcgcgtgg atacgcgtgt gaccagaccg ttttcccgtc aagcgtggtt tttacgatct
     3541 tgcagcgatt gatatcgcag tgatacaaaa actcgccgtc ggtctccctg cggatgctca
     3601 ggccatgagc gccgccgcga aattcttcgc cgaacgcact cacaaagcgg ccatcggcgc
     3661 cgtaaacgac aatcgcttcg cctcgcatgc tggacttgtt gaccgtgtgg gccacgtaaa
     3721 tgcgcccttg ctcgtcctga gcgaggccat gcgtgtctcc ccaaaccagc ccatcgggtg
     3781 gaataagcca gtcgtgcacg cattcatagg tgtgagggcc ggaaccaacc gtcggcaaac
     3841 gctggccgga tttgtccgat gtgtgcaggt aaaaaggtgg gattgcggcc gcagcggccg
     3901 ctgccgagca gcgagctaga aacgaccgtc gagagcgaga tctcatgcga ttagttccgg
     3961 cacgactttt gccggaacca cccccgtcaa tttttgctcc agaccttgaa acttgaattt
     4021 cagcttttcg tgatcgatgc ccagcaagcg cagcacggtc gcattgaagt cgcgcacgtg
     4081 gactgggtct ttcaccacgt tatagcaaaa atcgtcggtt tgtccgtgaa caagtccggc
     4141 ttttgtaccc ccgcccgcca tccacatggt gaaacaacgc gggtgatgat cccgcccata
     4201 gttttccttg gagagacctc cctgactgta tacggtgcgc ccgaattcgc cgccccatag
     4261 gaccaatgtg tcatccagca ggccacgacg ccgcaaatcc gtgaccagag cgtagcaggc
     4321 acgatcggtg gctgcgcaaa gcttgggcaa atttccgacg gcgttcgagt gcacatccca
     4381 tccacgctga taaatctggg tgaagcggac tccacgctcc gccatgcgcc gcgccagcag
     4441 gcagttgtac gcgaacgtgc cggcttgctt tgcttcgtcg ccgtacaaag tccacgtact
     4501 atccggttcg tcacgcatat ccgtaagttc cggcagagaa acttgcatgc gaaacgccat
     4561 ttcgtattga tgaatgcgcg catgagtctc cggatcacca acttcttcaa aggtcatctg
     4621 gttgatcgaa ttaacagcgt cgatcagcgt acggcgggtt tcccggccca ttccttgcgg
     4681 atcgcccagg taaagtactg gctcgccctg agaacggaat gcaaccccgg cgtactcgtt
     4741 cgacagaaat ccgctgctcc acaatttcgg tgatatgggc tgcaggtctg tgccaggcaa
     4801 cgtaagcgaa ttcagcacca caaaggtcgg caggttttca ttcatgctgc caagcccata
     4861 agaaagccac gagcccatcg acggcttgcc gggcaccata tttccggtgt tcatcaacaa
     4921 gattgccggt tcgtgattaa tggcgtccgt ctcgagcgat tggatcagtg tcagctggtc
     4981 cactagcctt cccgtccacg gcagcagatc gcttacccag cgcccagatt tgccctggcg
     5041 agtgaatccc caatgggacg gcgcaatcgg aaagcgcgcc tggccggaag tcattcccgt
     5101 tagcgcctgg gtgccacgca cggaatccgg aagatcctta tcataaagac ccgcgagacc
     5161 cggcttgtag tcccacagat ccatttgcgg gggcgcgccg gacatgaaga gatagatcac
     5221 gcgtttagct tttggcgcaa agtccggcac atagtgcgat ggcgaacctg gctgggaatc
     5281 agctgaggcc cgagcgactc gcgccgctat tacattgcca aacaacgttg ccaggcttgc
     5341 ccagcccagc gtcttgccca tcttgccgag gaagtgcctg cgcgtagcca gggttcgcca
     5401 ttcttcgcgg agttcaagcg ggatcgggag gtcgtaaacc gtgggcccgg cgccgccatg
     5461 atcacaacag tgtccgcttt gctcgtgatt cttcgctttc atttgtttag caccgcatca
     5521 agattcattg ctgcgctggt taccagcatc cacgaagcca gctcatccga acggattccc
     5581 gtgtcccggc tgctctcgcc cactttcaaa atctcctctg cccgcacctg atcatgctcg
     5641 taggtacttt gaaacttgtg cagcgcattc tcaaggaccg atttttcccg cggttgccaa
     5701 tcccgcgcca gcagaacttc gccaagatag tcgagacggg tagaagtgtc ggaggagtgc
     5761 cggattaccc gctcggcaag ccggcgcgat gcttcgagcc actgcggatc gttcatcaag
     5821 accaacgctt gcaacggagt gtccgtgcgt tggcgccgcg tacaagcggc atcgcgcacc
     5881 ggcacatcca gtgtgtccat atcgggcatc gtcgccattc gtttccaaaa agtgtaaagg
     5941 ctccggcggt acagcgaatc accgtggtct tgcgtataac ttttggtgtc gctgccctga
     6001 tggcttccgg cttcccacac tccagcgggc tgatatggct ttacgctagg gcctccaatt
     6061 ttttcgacaa gcaaaccgct agtcgccagc gccgtgtcgc gcagcatctc gccgtccatc
     6121 cgatagcgtg ggccgcgggc cagcaggcgg ttcttgggat cgttctcgag caactgcggc
     6181 gttacctggg cggattgccg ataggtcgca gaaagcacca gttgccggta aaagcgtttc
     6241 acctcccagc cgttcgcgcg aaaatccagg gccagccaat ccaggagttc cggatggctg
     6301 ggccgcccgc ccatcactcc gaagtcctct gtagtttcga cgaggccggc tccgaaaatc
     6361 tccgcccaca tccggttcac agtcactcgc gctgtcagcg gattagaggc gcttacggtc
     6421 cactcggcca agcccagccg gttgagcggc gcattcgcgg ggagcggggg caagaagtgc
     6481 ggcacgcccg ggcgcacgcg ttcggtgcgg gcgccgaacg cgccgcgggt caacacgtcg
     6541 gcgtagggta aacccggccc ttcttcactc accagagtga tggccccgtc tttcgaaagg
     6601 cgctcaagtt cttcattcaa aggcgcgatc tgggccctca gtttttgcgt aacatcatcg
     6661 cgctgcgcaa aataaaactc gctgaccgta tggaattcgt cctccgacca cgacgacatc
     6721 ggtttgcgta cgatttcgga tacatagtct tcgacaggaa gcctcgaagc ctcgctcgtc
     6781 gagagtgcgc gattgaagaa tcgaaaatcc tgaaacgcag tttggcgcaa gggatcggaa
     6841 tccgggtaca tacggccaaa ctccaccggc gcgctggttc gcgtactacc ctgtagtgaa
     6901 tctttgacga cggctaattc ctgcgctcgt ccatcgatat ataacaccac acctgcggcc
     6961 ttgcccgagc catcgtaggt ggccaataca tgattccagc ggccaaccgt caatactttc
     7021 agtttggtgg cgacttgaat cgcgttatcc ggcatttcgt tcgccagaac gaattcgagc
     7081 ttgcccttgc tgactcgcaa ctgccagccg cgctgctttt gccctgaatc cgcggccgat
     7141 agaattacgc ctgcgggctt ttcctcgtcc ttcgcttcga gggatcgtaa gtgcggccgc
     7201 acccaagttg ccgcgctgaa cggctgattt gcctcaactt ctcctgcgct tggcaattcc
     7261 aacttggtgc tgatctccat ccgcatatac ggccagaacc aggtgccttc gccccagact
     7321 accggtgctc ccgttgccga aacatgcttg tgcgaagcgc ctggcgccga gtccgcaaac
     7381 gtcgatccat tctgttcgtc gaagcgcaac ctgaccgcga ggccctctga acttacgggc
     7441 actgcggcag aagtgcgctg gccaatccag gcaccaatca gttcgtgggc ttgttcgcgg
     7501 tgaacggcca ttcgccgctc gagttccgag cgcttggcca gcgtttgctc gtaactatcg
     7561 cggttctctg cctggggaag gcgaagaaac ggtggccagt cattgcgatc atcgttcgaa
     7621 ggaatttccg tcagattgtt gaagaaggcg gtcagtcgat agaaatcttt ctgcgtaatc
     7681 gggtcaaact tatggtcatg gcaattagcg cagcctactg tcagcccgag aaacgctgca
     7741 ccgtatgctt ctgttcgttc ccgcttgttg ttgactcgca attcctcgat cagcgtgccg
     7801 ccttcgccgc tgctgatccc tgcgcgcagg tatgccgagg ccgtcaggct atccagcgtt
     7861 ttcaccggta gcatgtcgcc ggcaagttgc tcgcggacaa attgatcgaa aggtttgttt
     7921 tcgttgtacg agcggattac ataatcgcgg taagcccaga tgcttctgaa attgtcgaga
     7981 tgcagaccgt gtgtgtcgcc gtaccgggcg acgtccagcc agtagtgcgc tcgatgttca
     8041 ccgtagcgcg gactggccag caagcgatcg acaactttct cgtacgcgtc gggtgctgag
     8101 tccgcaacga agccttggac ttcttccggc gtgggcggta atccagtcag atcgtaggtc
     8161 actcgacgaa tcaagcttcg ccgatctgct tccggagaag gtttcaaccc ttctttttcg
     8221 agctgcgcca gcacaaagtt gtcgatggcg ttccgtactg attcactgtt ctgcaccacc
     8281 ggaaccgctt gctgctttgg cggaaggaag gaccatagtt cttcgtactg tgcgccctgc
     8341 tttacccact ctctgagcag cgcgatctga tctggcttta atgttttgtg cgcctccggt
     8401 ggaggcatgc gttccttcgc attctgggcc tcgatacgct gaaccagcgg gcttttgtcc
     8461 ggcttgccgg gaagaattgc cggcccgtat ttctcgtggg aatcctgatg aggagcaagc
     8521 gaaaactcgc ttcggtccag ccgtaggcca gctttccggc ctcctggatc cggaccatgg
     8581 catgcgtaac agttttccga caggattggc tggatggtgc cattgaacga cagcttttgg
     8641 ggattttcag gaacggcagc gggttttgct tcacgtgact gggcggcggc gcaggggttc
     8701 aggcagagcc accccgcaag cacgtataag gctgtgccat agagtggcga tccggatctt
     8761 cgaccgcgct gactcagcac catttagttt ccccggggga ttttactgcc tgtgacgccc
     8821 tagcttcaca cactatatta cacccacacg tatcggagcc ctgcgtttcc tcgccgcgac
     8881 ctgttttccg acgagccata ccaatcttca cctacactcc tcacgcacgc agaaccgagg
     8941 cggggcgccc gacgttgccg aatgcgccgc gcccaaggga gctcacggct gcctatcaac
     9001 tgacttttta tttgctattg agaaatttta acgttgcgac catttgatgg ggttcgtttg
     9061 cgaataggcg gtgacg
//
LOCUS       DASTPM010000205         5771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1016083, whole genome shotgun sequence.
ACCESSION   DASTPM010000205 DASTPM010000000
VERSION     DASTPM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016083"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VFR42_03590"
     CDS             <1..466
                     /locus_tag="VFR42_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368267.1"
                     /translation="AGLLVAFLFTFSKVLFTAAEGAEGWTPALTVLLVRDVRFVQVAA
                     TLILVAVVLVYGIPMSRNLKGIILGYGLYLSLVVLNLTYLGVFGERVQFVVTKIQACS
                     YLLALLAWNAALWSYQPIESRAFVAQKSYSAICEETNEQLARTKNSVESVLP"
     gene            463..1017
                     /locus_tag="VFR42_03595"
     CDS             463..1017
                     /locus_tag="VFR42_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368268.1"
                     /translation="MTEYALMAAVTMSALLFVALFRRATRDAREEAQAGFRGLNDAGD
                     WWEHGSYVSREVLQQIFSQDDENFLNGEADPGTKFFFRRERKKLALRWIERRKLEAAA
                     VMRSHRQAARGAEDLQPSEEMRLLLRHAKLKIMCEALAVTVWLAGPQSLHGLAQRADE
                     IFHGAQRLRQAVRSRGDASAQQAQ"
     gene            1034..1552
                     /locus_tag="VFR42_03600"
     CDS             1034..1552
                     /locus_tag="VFR42_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368269.1"
                     /translation="MNGATLNRVLPADAWRVYDDFRTRTLARIPSALERLIYIASTRD
                     YNSGVYQHEGLAGRFGADAAAQALEYAHWEVFQSVSLLSLRRLTEELHKYMQSSREQP
                     IAFLNAWQKLEPYRVAIPLRVDPTIAELFISNIKIALAVLRHRQSAKPDRPQGALPPP
                     SPAPRSPLPYRG"
     gene            complement(1437..2252)
                     /locus_tag="VFR42_03605"
     CDS             complement(1437..2252)
                     /locus_tag="VFR42_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0368270.1"
                     /translation="MLPSKPSDQLKEIRAKLGITTRDVAVLSQRLAEVENNPEYAISN
                     AWLTQVENSTSVPSIYKLYALSAIYRIKFTDLLLLFGVDLQKLGHHQKELQLPNTHLV
                     TMEVYDSEKTVSFPIRFDRSFDIENTNLLNRMVEIWGEVPISLIQHLDLRHSLYGYVG
                     LRDFTLYPLLRPGSFVQIDQKVRKIRPARWRTEHDRPIFFVELRDGYACSWCEIQDGY
                     LLLLPHPLSPVSVRRFIHGTEAEIVGQVTAVAMRLADDPASQTDDAARLPKRS"
     gene            complement(2409..3005)
                     /locus_tag="VFR42_03610"
     CDS             complement(2409..3005)
                     /locus_tag="VFR42_03610"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0368271.1"
                     /translation="MKRHGREELTTADLVLLSLLAEQPMHGYQANRELQRREIRDWAA
                     ISRPQVYYSMEKLARLGFLRKVQGGAASGGPEKQSFTATKKGVAALADALEREDWCTQ
                     REKPPFLTWLALTWQARSGVFHRQLRRREQFLKHELQRARATLRSVLEEVGHPYHEAV
                     WMLKLMVAQFSIELRWLGELRKQSRRRARARHPTLETP"
     gene            complement(3002..3307)
                     /locus_tag="VFR42_03615"
     CDS             complement(3002..3307)
                     /locus_tag="VFR42_03615"
                     /inference="COORDINATES: protein motif:HMM:NF023159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU0368272.1"
                     /translation="MKTQRRELKDLISVGPATLRDFDRLGIRSVAQLATQNPQGLYDK
                     FNRLSGHSHDICLLDVFRSAVAQARNPRLRPEQCQWWWWSKKRKRAAAAKLGKESSV"
     gene            3384..3776
                     /locus_tag="VFR42_03620"
     CDS             3384..3776
                     /locus_tag="VFR42_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0368273.1"
                     /translation="MPVKKLTPVLFVEKIEAVLPFWTQHLGFIKTAEVPDGARLSFVI
                     LQQGTAEVMYQSYASVEKDMPAILADVRKGPSFLYLEVDDLQTIRGALKGAETYMPER
                     TTFYGAREIGVKDPSGHFITFAQFEAPA"
     gene            3942..>5771
                     /locus_tag="VFR42_03625"
     CDS             3942..>5771
                     /locus_tag="VFR42_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0368274.1"
                     /translation="MISCYLRPPAAPAALAQAPSGAAPAPAFDAVAFIRVAPDGIVTI
                     LSKNPEVGQGVKTHLPMILADELDVDWKDVRIEQADLDETKFGPQRAGGSTSTPTNWD
                     PLRRAGAAVRQMFVTAAAEKWSVPAAECSTASGRVLHGASNRSVGYGELAGKVATMSP
                     PDSQTVKPKDPKDYKIIGKPVRSVDNPKIVRGKQTYSIDTKVPGMLYAVFEKCPVFAG
                     KIASANIDEIKTMPGVRHAFIVDGTQELLGLHCGIAVVGDSWWQAQSARKKLRVTWNE
                     GTTAEQSSAGFAKRADELSKQPPVFAVRADGDADKALQSAAKVVEASYAYPFIAHAPL
                     EPQNCLAHYKDGKLEFWSPSQTPGRGRAQVAQLLKISENDITLHICRIGGGFGRRLTN
                     DYMLEAAAIAKEVGVPVKLLWTREDDFHHDHYRPIGFHYLKAGVDGSGKVTAWRNHFV
                     SLGQDKQFAPTAGIGPGEFPASFVPDYLFAASLMPCGVPTYALRAPGSNAYAFVFQSF
                     LDEVAHAAGKDPIAFQLDLLSAPRVRNAANTPAQGQPEFDAERMRAVLAQVAKESNWG
                     QRKLAKGTAMGAACYFSHRGYFAEVAEVRVDSGKKVKVNKVWVV"
BASE COUNT         1194 a         1489 c         1878 g         1210 t
ORIGIN      
        1 ggccggactc ctcgtcgcct tcctatttac gttcagcaag gttttattca ccgccgccga
       61 gggtgcggaa ggctggaccc ctgcacttac cgtccttctc gtgcgagacg tgaggttcgt
      121 acaagtagcg gcaacgctta ttctggtggc cgtagtgctg gtgtacggca ttccgatgag
      181 ccgaaatttg aaggggatca ttcttggata tggattgtat ttgagcctgg tggtgctgaa
      241 tctgacgtac ctgggtgttt ttggcgagcg cgtgcagttt gttgtgacga agatccaggc
      301 gtgttcgtat ctcttggcgt tgctggcatg gaatgcggcg ctgtggtcct atcagccgat
      361 tgagagccgg gcgttcgtag cgcagaagag ttactccgcg atttgcgaag agacaaacga
      421 acagttggca cgaaccaaga attcggtgga aagcgtgctg ccatgacgga atacgcgctg
      481 atggcggcag tgacgatgag cgcactcctg ttcgtggcgt tattccggcg cgcgacccgg
      541 gatgcgcgcg aagaggctca agccggattt agaggcttga acgacgcggg cgactggtgg
      601 gaacacggaa gctatgtgtc acgcgaagtt ctgcagcaga ttttttctca agatgatgaa
      661 aacttcctca acggagaagc cgatccggga acgaagttct ttttccggcg ggagagaaaa
      721 aaactcgccc tgcggtggat cgagcgcaga aagttagagg ccgcggctgt gatgaggagt
      781 caccggcaag cggcgcgagg agccgaggac ttgcagccat ccgaggaaat gaggctgttg
      841 ctgcggcacg cgaagctgaa gatcatgtgc gaagcattgg cagtgaccgt atggctggcg
      901 ggaccacaaa gtttgcacgg gctcgcgcaa cgggcagatg agatttttca cggcgcacaa
      961 cgtttgcggc aagcagtaag aagtcgaggg gacgcctcgg cgcagcaagc gcagtaacta
     1021 gcgacgggat cgaatgaacg gagccacgtt gaaccgagtg ctaccagccg atgcctggag
     1081 ggtgtacgac gatttccgca cgcgaacatt agcgcggatt ccgtcggcgc tggagcgact
     1141 gatttatatc gcgtcgacgc gtgactacaa ctccggtgta taccagcacg aaggactggc
     1201 cggaaggttt ggagcggatg cggcggcgca agcgctggag tacgcgcatt gggaagtgtt
     1261 tcagtcggtt tcgttgctgt cgttgcggcg gctgacggaa gagctgcaca agtatatgca
     1321 atcgtcgcgc gaacagccga tcgctttttt gaatgcctgg cagaagctgg agccttaccg
     1381 agtggcgatt ccgctgcgcg tggatcctac gattgcggag ctatttattt ccaacatcaa
     1441 gatcgctttg gcagtcttgc ggcatcgtca gtctgcgaag ccggatcgtc cgcaaggcgc
     1501 attgccaccg ccgtcacctg ccccacgatc tccgcttccg taccgtggat gaaacgccgc
     1561 acgctgaccg gcgacagcgg gtgcggtaac aacaagagat agccatcctg aatttcgcac
     1621 cacgaacagg catatccgtc gcgaagctcc acaaagaaaa tagggcgatc atgttccgtg
     1681 cgccagcgtg ccgggcgaat tttgcggact ttctgatcga tctgcacgaa ggaacccggg
     1741 cgaagcagcg ggtacagagt gaaatcgcgc aggccgacgt agccgtagag gctgtggcgg
     1801 aggtccaggt gttggatgag ggagatgggg acttcacccc agatttccac catgcgattg
     1861 aggagattgg tattttcgat gtcaaaggaa cggtcaaagc gaatcggaaa agagacggtt
     1921 ttttccgagt cgtagacttc catagtgacc aggtgggtat tgggaagctg cagctccttt
     1981 tggtggtgcc cgagtttttg caaatcgacc ccgaagagca agaggagatc ggtaaacttg
     2041 atccggtaaa tcgcgctgag tgcgtaaagt ttgtagatgc tggggacgga agtggaattt
     2101 tcgacctgcg tgagccaggc attggagatg gcgtactcgg ggttgttttc gacttcggcc
     2161 agacgctggc ttaggacggc gacgtcgcgc gtggtaattc ccagtttggc ccgaatttct
     2221 ttgagctggt cggatggctt cgagggaagc atgctccccg agtgtaccct attggggcgg
     2281 cagaacgcat ccgtgcaaca aacgtttcat tggcggtagt gaatcaggca attggcggat
     2341 agcgaatttg tgtcgtctac aggaagcccg ggctggagtc agactttttc cacggccagc
     2401 cattttggct atggcgtctc cagggtggga tggcgggcgc gggcgcgccg gcgcgactgc
     2461 tttcgcagct cgccaagcca gcggagctcg attgaaaact gggcgaccat caacttcagc
     2521 atccacacag cctcgtgata cggatggccg acttcctcca agacgctgcg cagcgtagcg
     2581 cgtgcgcgct gcaactcgtg ttttagaaat tgttcccgcc gccgcaattg ccggtggaaa
     2641 actccgctgc gggcctgcca ggtaagcgcc aaccaggtta gaaaaggcgg cttttcgcgt
     2701 tgcgtgcacc agtcttcgcg ctccagtgca tcggcaagcg cggcaacgcc tttttttgtt
     2761 gccgtaaagc tttgtttttc cgggccgccc gaggctgcgc caccttgcac tttgcgcaag
     2821 aagccgaggc gggccaattt ttccatcgag tagtacacct gcgggcgtga gatggccgcc
     2881 caatctcgaa tttcgcgccg ctgcagctcg cgattcgctt gatagccgtg catgggctgt
     2941 tcggcgagca ggctgagcag caccagatca gcggtggtga gttcttcgcg accgtgccgt
     3001 ttcaaaccga ggattccttg cccaactttg cagcggcggc ccttttccgc tttttgctcc
     3061 accaccacca ttggcactgt tcggggcgca agcgtggatt gcgcgcctgg gcgacggccg
     3121 agcggaaaac gtcgagcagg cagatgtcgt gagagtgtcc ggataggcga ttgaacttgt
     3181 catacagccc ctgcggattt tgcgtggcga gctgcgcgac gctgcggatg ccgagccggt
     3241 cgaagtcgcg cagggtggcg ggcccgactg agatcaaatc tttgagttcg cggcgttggg
     3301 ttttcatggc gcttgtcctt gacagtctca ttttgtttca ctagtctaat ttagactagt
     3361 tagcgaaaca aggagattcc gccatgccgg tcaagaaatt aacgccagtg ctcttcgtcg
     3421 agaagatcga agcggtactt ccgttctgga cgcaacatct cggctttatt aagacagcgg
     3481 aagtgccgga cggcgccagg ctgtcgttcg tcattctgca gcaaggcacc gccgaggtca
     3541 tgtatcagtc ctacgccagc gtcgagaaag atatgccggc gatcttggcc gacgttcgca
     3601 aaggcccctc gtttctctat ctcgaagtgg acgacttgca gacgatcagg ggcgcgctaa
     3661 agggcgcgga aacctacatg ccggagcgca ccacgtttta tggagcgcgc gaaatcggag
     3721 tgaaggatcc gagcgggcac ttcattacgt ttgcgcagtt cgaggcgccg gcttgaccgg
     3781 ctggctatcg gtcagtgagc gggcttgcga gactctattg gcaccagtcg cgggcggcgc
     3841 tagaatcagt cttccagcca gcagcgaggt gcgcgatgca agccgcagtg ctcaatcgcc
     3901 gatcatttct gaaggttacg tctatcgcgg gcggaggagt tttgatttcc tgctatttgc
     3961 ggccgcccgc ggcgcccgcc gcgctggcac aagcgccgtc tggcgccgca cccgcgccgg
     4021 catttgatgc ggtggcgttt attcgcgtgg cgcccgatgg catcgtgacc atcctgtcca
     4081 agaatccgga agttgggcaa ggcgtaaaga cgcatttgcc gatgatcctg gcggatgaat
     4141 tggatgtgga ttggaaagat gtgcggatcg agcaagcgga cctggatgaa acgaagttcg
     4201 gtccacaacg cgccggaggg agcacgtcga ctcccacgaa ctgggatccc ctgcggcgcg
     4261 ctggcgccgc tgttcggcaa atgtttgtca cggcagccgc ggaaaaatgg tcggtgcctg
     4321 cagctgaatg ttcgacggct tcgggccgag tgctgcacgg ggcttcgaat cgttcggtag
     4381 gttacggtga actcgcgggg aaagtggcca cgatgtctcc gccggattcc caaacggtga
     4441 agccgaaaga tccgaaagat tacaagatta tcgggaagcc cgttcgcagc gtggacaatc
     4501 cgaagattgt ccgcggcaaa cagacttaca gcattgacac gaaagttccc ggaatgctgt
     4561 atgccgtctt tgaaaaatgt ccggtgttcg cggggaaaat cgcttcggcg aatatcgatg
     4621 agattaagac gatgccggga gtgcggcatg cgttcattgt agacggaacg caggagctgc
     4681 tgggactgca ttgcgggatc gcggtggtgg gggattcatg gtggcaagcg caatcggcgc
     4741 gcaagaagct gcgcgtgact tggaatgaag gcactacggc ggagcagagc agcgcgggat
     4801 ttgcaaagcg ggcggacgaa ctctccaagc agccgccggt tttcgcggtg cgggcagatg
     4861 gcgacgccga caaggcgctg cagtccgccg caaaagttgt ggaagcgtcc tatgcgtatc
     4921 cgttcattgc gcatgctccg ctggagccgc agaactgcct ggcccactat aaggatggaa
     4981 agttggaatt ctggtcgccg agccaaacgc cgggacgcgg ccgagcacaa gtggcgcagc
     5041 ttttaaaaat ttcggagaac gatataacgc tgcacatttg ccggattggc ggaggttttg
     5101 gccgccggtt gacgaacgat tacatgctgg aagctgcggc gattgccaag gaagtaggcg
     5161 tgccggtcaa gctgctgtgg acgcgcgaag acgactttca tcacgatcac taccggccca
     5221 tcggctttca ctacctgaag gcgggcgtcg acggttccgg gaaagtgacc gcgtggcgaa
     5281 atcatttcgt cagtctgggg caggacaagc agtttgcgcc gactgcgggg atcggtcctg
     5341 gagaatttcc cgccagtttt gtgccggatt acctttttgc ggcatcgctg atgccatgcg
     5401 gcgtgccgac gtatgccctg cgtgcgcccg gaagcaacgc gtatgcattc gtcttccagt
     5461 cgtttttgga cgaggtggct catgcggcgg gcaaggatcc gatcgcgttt cagctcgatt
     5521 tattgagcgc gccgcgcgtt cggaatgcgg caaacacgcc agcgcagggg cagccggagt
     5581 ttgacgcgga gcggatgcgc gcggtgctgg cacaagtggc gaaggagtct aactgggggc
     5641 agcgcaagct ggcgaaaggc acagcaatgg gcgcggcatg ctatttctcg caccgaggct
     5701 attttgccga agtggccgaa gtgcgcgtgg acagcggcaa aaaagttaag gtgaacaagg
     5761 tttgggtggt a
//
LOCUS       DASTPM010000206         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1304530, whole genome shotgun sequence.
ACCESSION   DASTPM010000206 DASTPM010000000
VERSION     DASTPM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304530"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR42_03630"
     CDS             <1..258
                     /locus_tag="VFR42_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368275.1"
                     /translation="VLACSALKQSYRDTLSAGLDFRIVYLRGTYEQMRQHILARHGHF
                     AGESILAGQFADLEEPRDALAIDVVGTADEIANQAIEKLKL"
     gene            374..1519
                     /locus_tag="VFR42_03635"
     CDS             374..1519
                     /locus_tag="VFR42_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368276.1"
                     /translation="MSNEVFSSRIQNSQNRSTHIVRWLTLAAALVAVSLFNSTQIKAQ
                     DQGGQDQPVPQSAQSQQPPAQPPDRQPAPPRRQDSSDSHPVTSRDTFPQDQEPNAASV
                     PANRHSHDRPWVAASAPANAPVPANLTISAGTVIFARLAEPLTSDHNHAGDTFTATLD
                     KPIIVDGWVVARRGETILGNVTTAQKAGRIKGVSQLGLELIDLTVVDGQQLPILTELW
                     KGSAGTSHGTDAAGVAATTGVGTIIGAAADGGAGAGIGAGAGAAAGLALVLLTRGKPT
                     VLGTEALLSFRLRQPLTVSTTRNQQAFLPVGPNDYSSPSLGRRDDAYVAYPPPYYAPP
                     YYATCGPYGCAPYYGYGYWPAFGFAYYGGYGYRGRFYRGGFGHGFHR"
     gene            complement(1542..2432)
                     /locus_tag="VFR42_03640"
     CDS             complement(1542..2432)
                     /locus_tag="VFR42_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0368277.1"
                     /translation="MATQEPAAPQLALLVEGDAEIEQALRKILELRGWEIVRAAGATE
                     ALEQVKLRQFGLIVTAGHTSGAEDVELLRKMRRVHAHTRMIIVTDESTPQDVLDSMRE
                     RAFSYFAKPFAMESFEHIVDIAINCPCWDDGIEVVSATPDWVNLQVRCDPNVADRLLQ
                     FMREISDLPDKEGRDAGVAFREVLLNAMEHGGHFDPEEYVEISYVRTRKMVTCRIKDP
                     GKGFSLQELKHSALANPPGEPLKHATVREEQGMRPGGYGVLLARHLVDEMLYNEKGNE
                     VFLVKYLGQARPWTGSAQSL"
BASE COUNT          460 a          933 c          663 g          512 t
ORIGIN      
        1 gtcctcgcct gctccgctct gaagcaaagt tatcgtgaca cgctctccgc cggcctcgat
       61 ttccgcattg tctatctgcg cggcacttac gagcagatgc gccagcacat cctcgcccgc
      121 cacggccact tcgctggaga atccattctt gcaggccagt tcgccgacct cgaagaaccc
      181 cgcgacgcgc tcgccatcga tgttgtgggc acagccgacg aaattgcgaa ccaggccatc
      241 gaaaaattga agctctgact tccgtccgcc gcaggcatcc agcccaccgc aacttctatc
      301 aaacttccgt gttaaacaaa tgcagtacct tcgccgtgcg aggtctggac ccttgccggc
      361 ttcagagaag cccatgtcaa acgaggtttt tagcagtcgc attcagaatt cgcaaaatcg
      421 ctccacccac atcgtccgtt ggctcacgct ggccgcagct ctggtcgctg tgagcctctt
      481 caattccacg caaataaaag cgcaagacca agggggccaa gatcagcctg ttccgcagtc
      541 tgcgcagtcg cagcagcccc ccgcacaacc acctgaccgg cagccagctc ctcctcggcg
      601 gcaagacagc agcgattcac atcccgtcac ttctcgcgac acattccccc aggatcagga
      661 gcccaacgcc gcttccgttc ccgcgaaccg ccattcgcat gatcgtccgt gggtggctgc
      721 gagcgcgcct gccaatgcgc ctgttccagc aaacttgacc atctccgccg gcaccgtcat
      781 cttcgcgcgc ctcgccgaac cgctcaccag cgaccacaat cacgcgggcg acaccttcac
      841 cgccacgctc gacaagccca tcatcgtgga cggctgggtc gttgcgcgcc gcggcgaaac
      901 cattctcggc aatgtcacca ccgcgcagaa agccggtcgc atcaaaggcg tttcgcagct
      961 gggactcgaa ctgattgacc tcaccgtcgt cgacggccaa cagctgccga tcctcactga
     1021 attgtggaaa ggttccgccg gcacttcgca tggcacggat gcggctggcg tcgccgctac
     1081 gaccggagtc ggcaccatca tcggcgctgc cgccgatggc ggagccggcg cgggcattgg
     1141 cgctggcgca ggcgccgccg ctggactcgc cctggtgctg ctcactcgcg gcaaacccac
     1201 tgtacttggc acggaggctc tgctcagctt ccgcctcagg cagccgttga cggtctccac
     1261 aacgcgcaac cagcaagcat tcttgccggt cggcccgaac gactactctt cgccgtcact
     1321 gggccgtcgc gacgatgcct acgtcgccta tccgccgcca tactatgccc cgccttatta
     1381 cgctacgtgc ggcccctacg gttgcgctcc ttactatggt tatggctact ggccggcgtt
     1441 tggctttgcc tactacggcg ggtacggcta tcgcggccga ttctaccgcg gcggcttcgg
     1501 ccacggcttt caccgctagc tggcggttga tgaacgccct ctcacaacga ctgggctgag
     1561 ccggtccacg gacgagcttg gcccaggtac ttcaccagaa aaacttcgtt ccctttttcg
     1621 ttgtaaagca tttcatcgac caggtgccgc gccagcagca ctccatatcc gcccggccgc
     1681 attccttgct cctcgcgcac cgtcgcatgc ttcagcggtt cgcccggcgg attcgccagc
     1741 gctgaatgtt tcagctcctg aagcgaaaat cccttgcccg gatccttgat gcggcacgtc
     1801 accatcttgc gcgtccgcac gtaggaaatc tccacgtact cctcagggtc gaaatgcccg
     1861 ccgtgctcca tcgcgttcag caagacttcg cgaaacgcca ctcccgcatc ccgtccctct
     1921 ttatccggca agtcggaaat ctcccgcatg aattgcagca gccgatccgc cacgttggga
     1981 tcgcagcgca cctgcaggtt cacccagtcc ggcgtcgccg acaccacttc gattccgtcg
     2041 tcccagcacg gacagttgat cgcgatgtcc acgatgtgct cgaacgattc cattgcaaac
     2101 ggtttggcga aatagctgaa cgcccgctcc cgcatggagt ccagcacgtc ctgcggcgtg
     2161 ctttcgtccg tcacgatgat catccgggtg tgcgcatgca cgcggcgcat cttgcgcagc
     2221 agctcgacgt cttccgctcc cgacgtgtgc cctgccgtga cgatcaatcc gaactgccgc
     2281 agcttcacct gctccagtgc ttccgtcgcg cccgcggccc gcacaatctc ccagccccgc
     2341 agctccagta tcttccgcag cgcctgctca atctccgcgt caccttcaac cagcaaggcg
     2401 agctggggtg cagccggttc ctgagtcgcc atgaaagctc cttgcggcgc gaatcagcat
     2461 cttacccgtt tctgccgccg ccccgcgaac tgcgttgcat ctccggtccg tctttctccg
     2521 tgggcaaccc tctgtcacca cggtgaatct ctcttgcagc ttgttacc
//
LOCUS       DASTPM010000207         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1102920, whole genome shotgun sequence.
ACCESSION   DASTPM010000207 DASTPM010000000
VERSION     DASTPM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102920"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFR42_03645"
     CDS             complement(<1..330)
                     /locus_tag="VFR42_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368278.1"
                     /translation="MRYLKLTIAYDGTDFHGWQIQTGKPTIQGEIVAILRRLTQENIA
                     IHGAGRTDAGVHALGQVASFRTQTALSTGELQRALNALLPQTIRIMAVEETGPDFSAR
                     WSARAKTY"
     gene            complement(351..971)
                     /gene="pyrE"
                     /locus_tag="VFR42_03650"
     CDS             complement(351..971)
                     /gene="pyrE"
                     /locus_tag="VFR42_03650"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU0368279.1"
                     /translation="MSATDQYVKIFEAAGAIRHGHFELSSGLHSATYVQCALVLQYPR
                     FAEKLGQALAALFSDTVIEAVVSPALGGVIIGQEVARALPEVPRGVAGGGVPALFVER
                     DASGTMTMRRGFSLQPDQHVLVIEDVWTTGGSTQEAIRVVQEAGGRVVAAGALIDRSG
                     GTIDFPVESQALLNLPIASYDPDECPLCREGSAAVKPGSRFVRSAP"
     gene            1241..3346
                     /locus_tag="VFR42_03655"
     CDS             1241..3346
                     /locus_tag="VFR42_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0368280.1"
                     /translation="MKQLVRPLMAIWLALFLAFGACSPSFAQSATQNPTTPAQTTVGT
                     GTLPPISLGMAKYNYSRAPKIFPNLIAPYTGGNIPSPSLINSPKIEQLIRDGRLEISL
                     QDAIELALQNSLDIEVARYNVWFADTDLLRTAGGGVGRGTAGASFPFSQANVPFLSFD
                     PTVTGTVSFDSQVVPVNNPFLSGTGTAALVTGLGIHTNQYNFGVSDGFSPGTTVSVNW
                     DNTRSSSSSPVNFFNPSVQSSLYVSISQQLLNGFGTFVNRRNIMISKNNRKIADLAFT
                     QQAITTVTATITAYYELAYARENVKVQQQAVTVAEKLYNDNKKQLEIGTMAPLDVTRA
                     ESELATDRQNLIVAQTAQLQQQQTLRNAISKDPMATSLVNVEIITTDKPTPPAIVEAA
                     SFEEAVKEAFIKRPDLLEQEINLTNAGIDAKANKKALLPIATVIARYGSQGLAGNSPI
                     LTSTPAAGGQIVDASGTPVTVIGAGGTPVAVFEPTTTTTVTGTKTGGLGDAQSQIFHN
                     QFPDYLLALNLNIPIRNRVAQADYQRAVLTQRQNEAQLQQLRNAAILDVRNTYIALVQ
                     NRARVEAAGKARELQQQTFDAEQKKYQLGASTVYNVILTQRDLITAQGTELRALADLV
                     EAKANYERALGRTLEVNNVTIAENGKGGAIEKDTLIPGTLNGQVVGTDKLFKKLDAQS
                     AVSAGASAAPAAATASGQK"
     gene            3553..>3982
                     /locus_tag="VFR42_03660"
     CDS             3553..>3982
                     /locus_tag="VFR42_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU0368281.1"
                     /translation="MSDSIKTKTLAPATAISPERKPSLEEKSWETNTLAPALAKSPER
                     QKEFTTLSGHPIRRLYTEADLANWDPKAQLAHPGEPPYTRGIHATMHRGRLWTMRQFA
                     GFGAAEDTNERFRYLLTHGQTGLSTAFDLPTLMGYDSDHPL"
BASE COUNT          861 a         1330 c         1014 g          777 t
ORIGIN      
        1 ataggttttg gcgcgcgccg accagcgcgc gctaaagtcc ggtccggtct cttccaccgc
       61 cataatacga atcgtctgcg gcaacaacgc attcagtgcc cgctgcaatt cgccagtaga
      121 aagcgccgtc tgcgtgcgaa aactcgctac ctgtcccagc gcgtgaacgc ccgcgtcggt
      181 tcgcccagcg ccatgaatcg caatattttc ttgtgtcaac cgccgtaaga tcgcgacgat
      241 ttcgccttga atggtgggtt tgcccgtttg aatctgccag ccgtggaaat ccgttccgtc
      301 gtaggcaatc gtgagtttga gatagcgcat gcgcgaacgt ggtcaggatt tcacggcgcc
      361 gagcgcacaa accggctgcc cggcttcact gccgccgaac cttcgcggca cagcgggcat
      421 tcgtcagggt cgtagctcgc gatgggcagg ttgagcagcg cctgcgattc caccggaaaa
      481 tcaattgttc ccccgctgcg atcgatcaag gcgcccgccg ccaccacgcg gcctccggct
      541 tcctggacca cgcgaatcgc ttcctgcgtg gagccgcccg tcgtccagac atcttcaatc
      601 accaacacat gctggtccgg ctgcagcgaa aatcctcgcc gcatggtcat ggttccgcta
      661 gcatcgcgct ccacaaacaa tgcgggaaca ccgccgccag caacgccccg cggaacttcc
      721 ggaagcgccc gcgccacttc ctgcccgatg atcacgccgc ccaaggccgg cgaaaccaca
      781 gcttcgatca ccgtatcgga aaacagcgca gccagcgctt ggccgagctt ttccgcaaag
      841 cgcggatatt gcagcaccag cgcgcactgc acataggttg cgctgtgcaa tccgctggac
      901 aattcaaaat gcccatgccg gatggctccg gccgcctcaa aaatcttcac gtattggtct
      961 gtcgcgctca tcctcaggct aaccgttgaa ttcctctaaa cgtcgaaatg tcaggagctt
     1021 agtggctgcg ccgtcgcgtt gtcaaggaat cgaggcgctg ttagactctt tcattccgcc
     1081 ccacggtgcc ctgcatcgat tccggtgccc ggcgggacgc cttctcctgc cggtttcatg
     1141 aaacaaatac ggagctgcac gcgttttaca tagtgaagat attcagaatc agcaccttaa
     1201 gtcccacggg acatctactt gaggggataa gcacaacatg atgaagcagc ttgtacgacc
     1261 gctgatggcg atctggctgg ccttgtttct ggccttcggc gcatgctcgc cctcgttcgc
     1321 gcaaagtgcc acgcagaacc cgaccactcc ggcgcaaacc accgtgggca cgggcactct
     1381 gccgccaatt tctttgggca tggcgaagta caactactct cgtgcgccca aaatctttcc
     1441 caatctcatc gctccttaca ccggcggcaa tatcccttcg ccgagcctca tcaattcccc
     1501 gaagattgaa caactgatcc gcgacggtag gctcgaaatc agcttgcagg acgccattga
     1561 gctcgccctg cagaacagcc tggacatcga agtcgctcgc tataatgttt ggttcgcgga
     1621 caccgacctg ctgcgcacgg ctggcggcgg cgttggccgc ggcaccgcgg gggccagttt
     1681 tcctttttcg caagccaacg tgccatttct aagctttgat ccgaccgtaa ccggaactgt
     1741 ttcgtttgat tcgcaggtcg ttcccgtaaa caaccctttt ctctcgggta ccggaacggc
     1801 ggctttggtc accggcctcg ggatacacac caatcaatat aattttggcg tgtcggacgg
     1861 tttttcgcca ggcaccaccg taagtgtgaa ctgggacaat acacgcagtt cctccagctc
     1921 tccggtcaac tttttcaatc cttcggtgca gtcatcgtta tacgtcagca tctcgcaaca
     1981 gttgctcaac ggcttcggca ccttcgtcaa ccggcgcaac atcatgatct ccaagaacaa
     2041 ccgcaagatc gccgacctgg ctttcacgca acaggccatc actaccgtca ccgctaccat
     2101 cactgcttac tacgaactcg cctacgcccg cgaaaacgtc aaagtgcagc agcaggccgt
     2161 gaccgtcgcg gaaaagcttt acaacgacaa caagaagcaa cttgagattg gcaccatggc
     2221 tccactcgat gtaacccgcg ccgagtccga gcttgctacc gaccgtcaga acctgatcgt
     2281 cgcgcagacc gcacagcttc agcagcagca gactctgcgc aatgccattt cgaaagaccc
     2341 gatggccacc agtctggtca acgtcgaaat cattaccacc gacaagccca cgccgccagc
     2401 catcgtcgaa gccgcttcgt ttgaagaggc cgtcaaggaa gcgttcatca agcgtcccga
     2461 tttgctagaa caggaaatca acctcaccaa tgccggcatc gacgccaagg ccaataagaa
     2521 ggcgcttttg ccgatcgcca ccgtcatcgc gcgttacggc tctcagggtc tggcgggtaa
     2581 ttctcccatt ctcacgtcca ctcctgccgc ggggggccaa atcgttgatg ccagtggcac
     2641 gcccgttaca gtgatcggcg ccggcggcac tccggtggcg gtttttgagc ccactaccac
     2701 caccaccgtg accggcacga agaccggagg tttgggcgac gcgcagagtc agatatttca
     2761 caatcagttt ccggactatc tgttggctct caacctcaac atcccgattc gcaaccgcgt
     2821 agcccaggcc gactatcaac gcgccgtact tacgcagcgt cagaacgaag cacagctgca
     2881 gcagctgaga aacgcggcga ttctcgacgt ccgcaacacc tacatcgcgc tcgtccaaaa
     2941 ccgcgcccgc gtggaagccg ccggcaaagc gcgcgaactc cagcagcaaa cttttgacgc
     3001 cgaacagaaa aaatatcagc tcggcgcgtc caccgtgtac aacgtcattc tgacgcagcg
     3061 cgacctgatc accgcgcagg gcaccgaact acgcgccctg gccgatctcg tcgaagccaa
     3121 ggctaactac gaacgcgcgc tcggccgcac cctcgaagtc aacaacgtga ccatagccga
     3181 aaacggtaaa ggcggcgcga tcgaaaaaga cactttaatt cccggcacct tgaatggtca
     3241 ggtggtcggc accgataagc tcttcaagaa actcgacgcg caaagcgccg tgagcgctgg
     3301 tgcgtctgcc gctccggcag cggcaaccgc ttcgggccaa aagtagattt cactcgattc
     3361 cgtcggggag gcgagcagct tggttgcttc cccgaaaacg ccggtctttt gatcggcgtt
     3421 tttctcttgc aggccgtgtt gcggcccggg cccgaccctc tttctcgcgt cacggttgcc
     3481 cgctcacccg ctttcgacta gcataagaaa ttgttattgc agcgatgcgg tcggcgaatc
     3541 ctcgccgctt ccatgagcga ctcgattaaa accaaaacac tcgcaccagc cacggcaata
     3601 tctccggagc gcaagccttc tctcgaagaa aaatcctggg aaaccaacac gctggctccc
     3661 gcccttgcca agtcccccga acggcaaaag gaattcacca cactttccgg ccacccgatt
     3721 cgccgcctgt acacggaagc cgacctggcg aactgggatc ccaaggcgca actcgctcat
     3781 cctggcgaac ctccttacac ccgcggaatt cacgccacca tgcaccgcgg ccgcctgtgg
     3841 accatgcgcc agttcgccgg cttcggtgct gcggaggata ccaacgagcg cttccgctac
     3901 ctcctcaccc atggccagac cggcctctct actgcattcg atctgcccac gctcatgggc
     3961 tacgactccg accatccgct ct
//
LOCUS       DASTPM010000208         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1333272, whole genome shotgun sequence.
ACCESSION   DASTPM010000208 DASTPM010000000
VERSION     DASTPM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333272"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1494)
                     /locus_tag="VFR42_03665"
     CDS             complement(67..1494)
                     /locus_tag="VFR42_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0368282.1"
                     /translation="MQATKTVAKEAARGVAAHILVVDDERSICELLEITFRKEGHRVE
                     VAHNVESARRKLESQIFDIVISDVKMPGEDGVELLKFAKEIAPECFFLLVTGVPTLDT
                     AIAAINAGADRYVIKDHELVDQLRRAVRSVAESLKWKKEAGYLRRELRRLTGLDNIIG
                     QSAKMRAIFDLIQTVAPQTSRVLITGESGTGKELVARAIHENSSRAQAPFITINCGAF
                     PETLLESELFGYMKGAFTGANENRQGLFQAAHGGTLFMDEIGNMTLTMQVKLYRVLQE
                     GKVRPLGSTEEVDVDVRVIAATNKDFEKEIAEGRFREDLYYRLSVIPLQLPSLRERRE
                     DIPLLARHFLERFRKSMEKPIGGISPEAMRKLESYEWPGNVRELENTMERAVALESGN
                     EISLRVLPDRIAGYASTPGLNSSNGSSQPFPEAGVDFEKEIADTERRYLLAALEKAGG
                     VRTRASELLKITYRSFRHYAKKHGL"
     gene            complement(1503..>2074)
                     /locus_tag="VFR42_03670"
     CDS             complement(1503..>2074)
                     /locus_tag="VFR42_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0368283.1"
                     /translation="GRMAPLEEDEKHLVNIVGRESERLNRIITDFLNYSREKSYEFRD
                     EDVRTILDDALTLMEKKPDVGSKYQIERAYNGKAVKARVDASKIKQVFWNLCDNALRA
                     MPQGGKLTVRLEERATWLRIAFRDTGFGLDARQKAKIFEPLQSGFEGGTGLGLSIVYQ
                     IVQAHNGRISVISERDKGAEFIVELPRAL"
BASE COUNT          390 a          684 c          497 g          503 t
ORIGIN      
        1 ctcagcagat cagcacagag ggagtccaat cgcagtctta aagccgatcg gccggttccg
       61 tcgcgatcac aggccgtgtt tcttcgcgta gtgccggaac gatcggtaag ttatcttcaa
      121 caattcagaa gcgcgcgttc gtacgccgcc ggctttctcc agcgctgcca gcaagtaacg
      181 ccgctccgtg tccgcgattt ccttttcgaa atccacaccg gcctcgggaa acggttgcga
      241 actcccgttc gacgagttga gtcctggcgt gctggcatag ccggcgatcc ggtcgggcag
      301 cacccgcaac gaaatctcat tgcctgattc cagggccacc gcgcgctcca tcgtattttc
      361 cagctcccgc acgtttcccg gccactcata ggattccagc ttgcgcatgg cctccggcga
      421 tatacccccg atcggcttct ccatgctctt ccggaaacgc tccagaaaat ggcgcgccaa
      481 caacggaatg tcttcccgcc tctcgcgcag cgacggtaac tgcaaaggaa tcacgctcag
      541 ccgatagtaa agatcttccc ggaaccgccc ttcggcaatc tctttctcga aatccttatt
      601 ggtggcggcg attacgcgaa catccacatc gacttcctcc gtgctgccta agggccgcac
      661 tttcccttct tgcagaacgc ggtacagctt cacttgcatg gtcagggtca tgttgccgat
      721 ttcgtccata aacagggttc cgccatgcgc cgcttgaaac aatccctgac ggttctcatt
      781 tgcgccggtg aatgcgcctt tcatgtaccc gaataattcc gattccagca aggtctccgg
      841 aaacgctccg cagttgatgg tgatgaacgg cgcttgggcc cgcgagctgt tttcgtgaat
      901 ggcgcgagct actaactcct taccggttcc gctttctccc gtgatcaaca cgcggctcgt
      961 ttgcggcgcg accgtctgaa tcaaatcgaa gatcgcgcgc atcttggcgc tttgcccgat
     1021 gatgttgtcc agtccggtca gccgccgcag ttccctgcgc aagtagcccg cctctttctt
     1081 ccatttcagg ctctcggcta cgctgcgcac agcccgccgc aattggtcca ccagctcgtg
     1141 gtccttgatc acatagcggt ccgcgcccgc attgatggcc gctattgcgg tatccagcgt
     1201 cggcacgcca gtgaccagaa gaaagaaaca ctcgggcgct atctctttcg cgaatttcaa
     1261 cagctccacg ccgtcttcgc caggcatctt cacatcggaa atgacgatgt caaaaatctg
     1321 cgattccagc ttgcgtcttg ccgattcgac gttgtgcgcc acctccacac ggtgcccctc
     1381 tttgcgaaag gtaatttcca gcaattcgca aatcgagcgt tcgtcatcca ccaccaggat
     1441 gtgcgccgca accccgcgcg ctgcctcttt cgctactgtc ttcgtcgcct gcatagaatc
     1501 ctttacaggg cccgtggcag ttccacgata aattcggcgc ccttgtccct ttctgagatc
     1561 acgctgatcc gtccgttgtg cgcttgcaca atttgataca cgatcgagag tcctagcccg
     1621 gtgccgcctt cgaagcctga ctgcaacggc tcaaaaatct tggctttttg cctggcatcc
     1681 aaaccgaatc ctgtatcgcg aaacgcgatg cgcagccagg ttgcccgttc ttccagtctt
     1741 accgtcagct ttccgccttg cggcatcgct cgcagcgcgt tgtcgcacag attccagaac
     1801 acctgtttga tcttgctggc gtccacccgc gctttcaccg cttttccgtt gtacgcccgc
     1861 tcgatctggt acttactccc aacatccggc tttttctcca tcaacgtcag agcgtcgtcc
     1921 agaatcgtcc gcacatcttc gtcgcggaat tcgtagctct tctctcttga ataattcaag
     1981 aaatccgtga tgattcggtt tagccgttcc gactctcgcc ccacgatgtt gaccaggtgc
     2041 ttttcatctt cctccagcgg agccatccgc ccca
//
LOCUS       DASTPM010000209         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_36013, whole genome shotgun sequence.
ACCESSION   DASTPM010000209 DASTPM010000000
VERSION     DASTPM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36013"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..784)
                     /gene="rpsD"
                     /locus_tag="VFR42_03675"
     CDS             complement(152..784)
                     /gene="rpsD"
                     /locus_tag="VFR42_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0368284.1"
                     /translation="MARHRDAVCRLCRREAQKLFLKGLRCFTEKCAIEKRNFVPGQHG
                     QSRRAKIAGYGLQLREKQKVKRTYGLLERQFRNYFEKAERTKGPTGENLIIMLERRLD
                     NVVCRAGLASSRAQARQLVLHGHVRVSGKKVNIPSYQVNVGDEIAIKDKMRDNAMVLD
                     ARNVAQSQNVAPWLEQDREQFRAKVVALPKRDDVQTPAINEQLIVELYSK"
     gene            complement(917..1285)
                     /gene="rpsK"
                     /locus_tag="VFR42_03680"
                     /pseudo
     CDS             complement(917..1285)
                     /gene="rpsK"
                     /locus_tag="VFR42_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
     gene            complement(1484..1873)
                     /gene="rpsM"
                     /locus_tag="VFR42_03685"
     CDS             complement(1484..1873)
                     /gene="rpsM"
                     /locus_tag="VFR42_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU0368285.1"
                     /translation="MARIAGVDLPVQKRLWVGLTSIYGIGQVRAKRLAEKAGVDHTKK
                     IRDLTEDEVNKLRNAIEQEGRVEGDLRKEIQMNIRRLIEIQAYRGIRHRRNLPVRGQR
                     THTNARTRKGPRRGAVAAKKQVKGKKG"
     gene            complement(1954..2064)
                     /gene="rpmJ"
                     /locus_tag="VFR42_03690"
                     /pseudo
     CDS             complement(1954..2064)
                     /gene="rpmJ"
                     /locus_tag="VFR42_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
     gene            complement(2137..2397)
                     /gene="infA"
                     /locus_tag="VFR42_03695"
     CDS             complement(2137..2397)
                     /gene="infA"
                     /locus_tag="VFR42_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0368286.1"
                     /translation="MAKHEDDKKKGDSGNPKEDAIEVMATVIEPLPNAMFRVELENKH
                     QVLAHVSGKMRKNFIRILAGDKVAVELSPYDLTRGRIVYRYK"
BASE COUNT          456 a          763 c          726 g          556 t
ORIGIN      
        1 tcaggcgcga gacgcttggg tttctggaaa cctttccaca tattcagttc cttccttctg
       61 gaagccggtg atgcggcttc cggtacttcg taaactcttc gtgctgcgtg ccaggctaaa
      121 gcctggcgct acatgacccg gcaaaagcgg tttacttcga atagagttcg acgatcagct
      181 gttcgttgat ggccggggtc tgcacgtcgt cacgcttggg cagagccacg accttggccc
      241 tgaactgctc gcgatcctgt tcgagccagg gagcgacgtt ctggctttgc gcaacgttgc
      301 gcgcgtcgag aaccatagcg ttgtcgcgca tcttatcttt gatggcgatt tcatcgccca
      361 cgttaacctg gtaggaaggg atgttgacct tcttgccgga aacgcggacg tgaccgtgga
      421 gcacgagctg gcgagcctga gcgcgcgaag acgcgaggcc ggcgcggcaa acgacgttgt
      481 cgagtcgccg ctcgagcatg atgatcaggt tctcaccggt tgggcccttg gtgcgctccg
      541 ccttttcgaa atagttgcgg aactggcgct ccagcaggcc gtacgtgcgc tttacctttt
      601 gcttttcgcg caactgcaag ccgtacccgg cgattttggc gcggcgcgac tggccgtgct
      661 gacctggaac gaagttgcgc ttttcaatgg cgcacttctc ggtgaagcaa cggagtccct
      721 ttagaaacag cttctgcgcc tcgcggcggc acaagcggca aactgcatca cgatgtcttg
      781 ccaacttctt ttctcccttc gcaaatcagt ggtccgggta ttgcgtaact gtcagcccgt
      841 accaaatttc ttttccacgg tgcgcgcctc gatgaggctt acatcgggcg cagacagggt
      901 tgtctgccct acatcatcag acgcggcggc gcttgggcgg acggcagccg ttatgcggaa
      961 tcggcgtgac gtcgcgaatc accacgatgt gcaagccagc cgcagcgagc gcgcgtacgg
     1021 cggattcacg gccggaaccg ggaccctgca cgcggacttc gacggacttg aggccgtact
     1081 gatcgcgcgc gttgctggcg gcgctgagcg aagcctgctg cgcggcgtac ggcgtgccct
     1141 tgcgcgaacc cttgaagccg atggcgccgg cggaagacca gcacagcgtg cggccgtcgg
     1201 tgtcggtgat ggtgatctgc gtgttgttga aggacgcagc cacgtgcacg acgccgtgcg
     1261 gcaccacgcg cttttcgcgc ttcttcttga attctttttt gcgcttggca gcggctccag
     1321 tggcggccgg ttttgccggt gccgctgttc cgtcaggcgc tgttttctgc tgctcttttg
     1381 ccacgtgatg ctccgtttcg taaaacttca aattcggagg ccaccggaag gtggccgcta
     1441 caaaatcccg caaagggcgg cgggacaaac tcgccgctac gaactagccc ttcttgccct
     1501 tgacctgctt cttggcagcg acggcgccgc ggcgcggacc cttacgggta cgagcgttgg
     1561 tgtgggtgcg ctgtccgcga accggcaggt tgcggcgatg acgaatgccg cggtaggcct
     1621 gaatttcaat caggcggcga atgttcatct gaatctcctt gcggagatcg ccttcgacgc
     1681 ggccttcctg ctcaatggcg ttacgaagct tgttcacttc gtcttcggtg agatcgcgga
     1741 tttttttggt atggtcgaca cccgcctttt cggcgagccg cttcgcgcgc acctggccga
     1801 tgccgtagat cgacgtgagg cccacccaca accgcttctg caccggaaga tcaacacctg
     1861 cgatacgtgc catgacttct cctcagaact gcaatcagaa aatcgaaccg caaccaccat
     1921 cgcccgcctc agaaggcggc cgctacaaga ctctccctgg cgctgcttgt gcttcgggtt
     1981 gacgcaaatt acccgaacca cgccgcggcg atggatcacc ttgcatttgt cacaaatctt
     2041 ttttaccgac gcgcgaactt tcattgcctg actccctttt gccaccaaca gatgccggct
     2101 taaaagccgg cacttcgtaa acccacaacc cggccgctac ttgtagcggt aaacgatgcg
     2161 accccgcgtc aaatcgtacg gcgaaagctc aacagcgact ttgtcaccag ccagaatgcg
     2221 aatgaaattc ttgcgcatct tgccggaaac gtgcgccaga acctggtgtt tgttttccag
     2281 ttcgacgcgg aacatcgcgt tcggcagcgg ctcgatcacg gtcgccatca cttcgatggc
     2341 gtcctctttc ggattgcccg agtcgccttt tttcttgtcg tcttcgtgct tcgccataag
     2401 ctcctgagaa aatcacgggg ctaaagaccc gccactacga aactcgcgga ggccagctgg
     2461 agctggccgc tacaacaccg caacggctac caggacgggc c
//
LOCUS       DASTPM010000210         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_180148, whole genome shotgun sequence.
ACCESSION   DASTPM010000210 DASTPM010000000
VERSION     DASTPM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180148"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFR42_03700"
     CDS             complement(<1..866)
                     /locus_tag="VFR42_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0368287.1"
                     /translation="MARVVQDVRGYRQANEDRIVRELREFLSIPNVASDTANIQKNAE
                     RLKEMLEARGVETHLLSIEARGPVVFGKLDTLGATRTIIFYAHYDGQPVVASEWTDKA
                     PFDPVLRSDSIEAGGGKIPFPDAGKNPSAYEDGWRIYARSAADDKSPIVAMLAALDAL
                     RAKNIPLAVNLKFVLEGEEEAGSEHLQQTLQLHKNLLKGDLLITGDGPVHQSGRPLVF
                     FGNRGDIGLDITVYGPVRSLHSGHYGNWAPNPALQLAQLLAAMKDENGRVAIPGYYDD
                     LVPFTALEVCAL"
     gene            complement(1000..2154)
                     /locus_tag="VFR42_03705"
     CDS             complement(1000..2154)
                     /locus_tag="VFR42_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0368288.1"
                     /translation="MKPGPLPSGALVPDDAGTEKLVKRPSSKSAAVTMRDVANASGFS
                     PATVSIVLNNAPLARYIAPATKKKIEDSARKLGYRPNVMARFLRSKRTHSVGVMFFDV
                     TDPFCTPILRGIENSLFQGSYVPILADAHNQRNRFERYLELLLERHVEALVVVANWLF
                     VDIHLLADFSKRNIPAATIGWELPGDTVSSVMVDNEAGGRLAMEHLHKLGHRKIAYIR
                     GPKTLIDSGPRWKGIQKFAHAAGLEIDSDLTMQLPEAFDPNSGFEGGFRLTEEFLQRR
                     KKFTALLAFDDLTAFGAIRALNKAGIRVPEQCSVIGFDDVALSALAAPSLTTVRQPLE
                     VMGNLAVNIIMEGIAASQEKREWKITHQRVAPELMIRESTQAIAISSSHD"
     gene            complement(2160..3527)
                     /locus_tag="VFR42_03710"
     CDS             complement(2160..3527)
                     /locus_tag="VFR42_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0368289.1"
                     /translation="MTNDPAGATRLRRSLTLWNLVIIGIVLIQPIAPMGIFGVVNNAA
                     RGHVVTTILIAMVAMLLTAISYGMLARIYPSAGSAYTYVGQELHSGLGFITGWAILMD
                     YILNPLICVILCSKLTQNILPEIPYWILAIFYAVAFTLLNLRGVKTSARINEVMAGAM
                     SIVVVVFFAYVVRFLIQLHNPEPHFFSKPFYDPAQFQLGLIFHGTSIAVLTYIGFDAL
                     STFSEEVENPRRNILLATVLVCLLTGILASLEVYAAQLVWGGKPFPANQVESAFPLVA
                     RQAGGVFLFQLLNFTILVANIGSGMGAQLAASRLLYGMGRSEAIPKGFFAKLHSKRGI
                     PANNVILVGALALAGALCDMLREPFRISYEKGAELLNFGAFIAFMGVNLAAFFRYFLR
                     AERKTAWTFAWPLLGFAICAYIWWELSKPAKILGAVWLAIGLGYGAWKTSGFRKSISF
                     DVPAE"
     gene            3797..>4252
                     /locus_tag="VFR42_03715"
     CDS             3797..>4252
                     /locus_tag="VFR42_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368290.1"
                     /translation="MGEGTLNPERWEATPVFWHVVALLLILLFALWLRPVPLHADPSL
                     LGYLQVVTGLLAFVFSAVTLVRFQGTQDRISLILGAGFLLSGAVLAGSSVLFFQIPAE
                     QPLRIQWAPVALWVGRIILGLLLVVALLVEHFLPRSRRPKIEIAGALLTV"
BASE COUNT          892 a         1290 c         1149 g          921 t
ORIGIN      
        1 tccagcgcgc atacttccag cgccgtaaac ggaaccaggt cgtcgtagta gccggggata
       61 gccacccggc cgttttcgtc cttcatggct gcgagcagtt gcgccaattg cagcgccgga
      121 ttcggcgccc agttcccgta gtggccgctg tgcagcgaac gaactggtcc atacacggta
      181 atgtcgaggc caatgtcacc ccgattgccg aagaacacca gcggccgacc ggattgatgc
      241 acggggccgt cgcccgtgat gagcaaatcg cccttgagca aattcttgtg cagctgcaag
      301 gtttgctgga gatgttccga ccctgcctcc tcttcgcctt caagaacaaa tttcaggttg
      361 actgcaagcg gaatattttt cgcacgcaat gcgtccagcg ccgcaagcat cgccacgatg
      421 ggggatttgt cgtctgcggc ggaacgcgca tagatgcgcc agccgtcctc gtaagcactg
      481 gggttcttgc cggcatctgg aaacggaatc ttgccgccgc cggcttcgat ggagtcgctg
      541 cgcaaaactg gatcgaaggg cgccttgtcg gtccattcgg aagcgactac cggctggcca
      601 tcataatgcg cataaaaaat aatcgtccgt gtggctccga gcgtgtccag tttgccgaac
      661 acaaccggcc cgcgcgcttc gattgacaac aggtgcgttt cgacgccgcg ggcttccagc
      721 atttccttca agcgctctgc gttcttttga atatttgccg tgtcgctggc gacgtttgga
      781 atcgacagaa attcgcgcag ctcacgaacg atgcggtctt cgttcgcctg gcgatagccg
      841 cggacgtcct gaacgacgcg agccacaggc ggaagcgttt tctttggttc tactcggcca
      901 ctcagcgatg actgcgcaac catcggtgcg cctgaacagg ccaacatcgc aaaactgcat
      961 aaaaagcgac tgcgggtgcc catggttctc cgtcaaaagc taatcgtgcg aagaggaaat
     1021 tgcaattgcc tgcgtggact cgcgaatcat gagctccggc gccacccgtt gatgagtaat
     1081 cttccattcg cgcttttctt gcgaagccgc gatgccttcc atgatgatgt tcaccgcaag
     1141 attgcccatc acttcaagcg gctgacgcac cgtggtcaac gagggcgcgg ccagcgccga
     1201 tagcgccacg tcgtcgaagc cgattaccga acactgctcc ggcacgcgaa ttcccgcttt
     1261 gttcagcgcg cgaatcgctc cgaacgcagt caagtcgtcg aacgccagca gcgccgtaaa
     1321 cttctttcgc cgctgcagaa actcttcggt caggcggaat ccgccctcga accccgaatt
     1381 cgggtcaaac gcttccggaa gctgcatcgt caggtccgaa tcaatttcca gtccggcggc
     1441 gtgcgcgaat ttctgaatgc ccttccaccg tggcccagaa tcgatcaacg tttttggtcc
     1501 gcgaatgtat gcgatcttac ggtggccgag tttgtgcaag tgttccatgg ccagccgtcc
     1561 gcccgcttcg ttatccacca ttacggaact cacggtgtca ccaggcagtt cccagccaat
     1621 cgtcgccgcc ggaatgttac gcttcgaaaa atccgccagc aggtgaatgt ccacaaacag
     1681 ccagtttgct acaaccacca gcgcttccac gtggcgttcg agaagcagtt ccaggtaacg
     1741 ctcgaagcgg tttctctggt tgtgcgcatc tgccagaatc ggcacgtacg atccctgaaa
     1801 cagtgagttc tcgatgccgc gcaaaatcgg cgtgcaaaat ggatcggtaa cgtcaaaaaa
     1861 catcacgccg acgctgtgcg tgcgcttgct gcgcaggaac cgcgccatca cgttcgggcg
     1921 gtaccctagt ttgcgcgcgg aatcttcgat ttttttcttg gtggccggcg caatgtagcg
     1981 ggccagcggt gcattgttca acacaatgga aactgtcgcg ggcgaaaagc cgctcgcgtt
     2041 cgccacatct ctcatggtca ctgcggcaga cttgctggat ggtcgcttca ccagtttttc
     2101 cgtcccagca tcatccggca caagcgcccc gcttggcaat ggcccaggct tcatcgccct
     2161 tactccgctg gcacgtcaaa cgatatggac ttgcgaaatc cggaagtctt ccacgccccg
     2221 tagcccagcc cgatcgccaa ccacacggca cccaaaattt ttgcgggctt gctgagttcc
     2281 caccagatgt aagcgcaaat ggcaaagccg aggagcggcc acgcaaatgt ccacgcggtt
     2341 ttgcgctcag cgcgcaggaa ataccggaag aacgctgcga ggttgacgcc catgaacgcg
     2401 atgaaagctc caaagttgag cagttccgcg cccttttcat aggaaatccg gaacggttca
     2461 cgcagcatgt cgcacagtgc gccggcaagc gccagcgccc caaccaggat cacgttgttg
     2521 gcggggatcc cgcgcttcga gtgcagcttc gcaaagaagc ctttcggtat cgcttcgctt
     2581 cggcccatgc catagagcag gcgcgaagct gcgagctgcg ctcccattcc cgaaccgata
     2641 ttcgccacca gaatcgtgaa attgagcaat tgaaatagaa aaactcctcc ggcctggcgt
     2701 gccaccaggg gaaacgcgga ttccacctgg ttcgcgggaa agggcttgcc gccccacacc
     2761 agttgcgcag cgtagacctc cagcgacgcg agaattcccg tgagcagaca aaccaggacg
     2821 gtggccaaca aaatattgcg ccgaggattt tcgacttctt cggagaacgt ggacaacgcg
     2881 tcgaatccga tgtaggtcaa caccgcgatg gaggtcccgt ggaatatcaa tcccagctgg
     2941 aattgtgcgg gatcgtaaaa cggcttgcta aaaaagtgcg gctcaggatt gtgcagctga
     3001 atcagaaagc gcaccacgta cgcgaagaac acgaccacca cgatgctcat ggctcccgcc
     3061 atcacttcgt taatccgcgc cgaagtcttc accccgcgca gattcagcag cgtgaacgcg
     3121 accgcgtaga aaatcgccag aatccagtag ggaatttccg ggaggatgtt ctgcgtaagc
     3181 ttgctgcaaa gaataacgca gatcagcggg ttgagaatgt agtccatcag tatcgcccaa
     3241 cccgtgatga aacctaagcc ggagtgcagt tcttgcccga catacgtgta agccgagccg
     3301 gcgcttggat aaattcgcgc cagcatcccg taactgatcg ccgtcaacag catcgcgacc
     3361 atggcgatca aaattgtcgt gaccacatgc ccgcgcgccg cgttgtttac cacgccgaag
     3421 attcccattg gcgcgatcgg ttggatcagc acaatcccga taatcaccag gttccagagc
     3481 gtcaagctcc ggcgcagccg ggtcgcgcct gcgggatcgt tggtcatcaa aatgtcctca
     3541 tatcatgttt gcctttgcgt gtctggaaga agacgccatt ctttgaaacg atttaagtca
     3601 cagattgtgc ggattttgcc gcgtccgcgc aactcttttt cttgccccgc cgcgtcccta
     3661 ggtgtcaact ccttacctgt tccaaaggac agtttgaccg cgcgcaacgt acagcaaaat
     3721 ccgataactc atctccctcg caatacagtc taatgaattg aacgcgccca actcccagtg
     3781 cgggccattt gcatctatgg gcgaggggac cctaaatccg gagcggtggg aagcgacgcc
     3841 tgtcttttgg cacgtggtgg cgctgcttct gattttgctt tttgcgttgt ggctgcggcc
     3901 cgtgccgctc cacgccgatc cttctctttt gggctatctt caggttgtta ccggcctact
     3961 ggcttttgtt ttttccgctg ttactctcgt tcgatttcaa ggcacgcagg atcgtatttc
     4021 actcatcctc ggcgccggct tcctgctgtc cggtgcggtc cttgccggct ccagcgttct
     4081 cttctttcaa attcctgccg agcagccgtt gcgcattcaa tgggcgccgg tggccttatg
     4141 ggtcggccgc atcattctcg gtttgttgct cgtcgtcgca ctgctcgtcg aacatttcct
     4201 gcctcgctcg cggcggccaa aaatcgaaat cgccggcgcg ttgctcaccg tc
//
LOCUS       DASTPM010000211         5280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1246985, whole genome shotgun sequence.
ACCESSION   DASTPM010000211 DASTPM010000000
VERSION     DASTPM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246985"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1759)
                     /locus_tag="VFR42_03720"
     CDS             complement(<1..1759)
                     /locus_tag="VFR42_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU0368291.1"
                     /translation="MNKLAAVISSVLLCFSFLFPANAKAQGAQAGVQFVDVTAKAGIR
                     FVHNNGAFGKKFLPETIGPGVAFIDYDNDGWPDIFLVNGTDWPGHVAKHTTPKLYHNN
                     HDGTFTDVTRKAGLDVEMYGIGVAVGDYDNDGYDDLFVTAYGQSRLFHNNGPDKTGNV
                     TFSDVTQKAGLSGVKEFSTSAAWVDYDKDGRLDLVVGNYVQWSPENDLYCTMDGKNKS
                     YCTPESYRGTSVRLWHNDGGGRFSDATQKSGLGEPTSKTLGVAMLDYDNDGWPDLLFS
                     NDTQPNKLYKNNGNGTFTEKAVVAGVAFSEDGVARAGMGVDTFDYDRSGNTSLMITNF
                     SNQMISLYHNEGKGLFVDEAPRSDVGRASLLTLGFGCFFFDYDLDGWPDILVANGHID
                     PDIQKVQVNVKFAEPPHLFRNLGKGKFAEVTKSAGAEFASARVGRSVAYADVFNDGRL
                     DVLFSTNAGAPHLFRNTAAGNANHSVRLKLTGTKSNRDGIGAVVKLTTPDGTQTQMLR
                     SGSSYLAANELALTFGLGQWSKADAVEIRWPSGQVDKLSDVSAGQTLMVTEGKGQTSA
                     RTFAKQSLPKTAEKFSSSGL"
     gene            complement(1756..2022)
                     /locus_tag="VFR42_03725"
     CDS             complement(1756..2022)
                     /locus_tag="VFR42_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368292.1"
                     /translation="MDDSLVEALRRDYHSAPITDQERVMCDYVAKLTKDATQCRPADL
                     DGLRAVGFDDKGILQITLIASWFSYINRVADALGVGRGDAAEKP"
     gene            complement(2059..2340)
                     /locus_tag="VFR42_03730"
     CDS             complement(2059..2340)
                     /locus_tag="VFR42_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368293.1"
                     /translation="MTWIKTVAMEDDPRVKAVVEAERKLYPQEYATPVPAVDRGFQNS
                     IVGSHTLLPDVLYHSFSAFGAMMAPELPLQRRQHEMIATMVSVTNRCVY"
     gene            complement(2389..>5280)
                     /locus_tag="VFR42_03735"
     CDS             complement(2389..>5280)
                     /locus_tag="VFR42_03735"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368294.1"
                     /translation="QNVSWGKRAMSRWRSILVLVLTLSAIYLYTFPAATIIYGGGVLL
                     HAGAGILLAVLLIPILRAVFRECSVGEKFGWVLLTAGTILGLALIKIGTPNRFKTWLY
                     LHIALCAAGVLLLASAWIARRGWLGKGFAGAAASFAVVALLFTGASAGAWWTRAVAWK
                     NAYRVVNPKMPAETMDGEGDGPNGKFFPSSVETRDGKNIPAKYFMQSQACERCHNDVY
                     KQWYSSAHHFSSFNNQWYRKSIEYMQDVAGVKASKWCAGCHDPALLFSGMFDTPIRQI
                     ENTPAGQAGLSCMMCHSIVKVKSTMGQGDFVLEYPKLHELAASENPVMRTLHDFMVKV
                     NPEPHRRTFLKPFMREQTAEFCSGCHKVHLDVPVNRYRWVRGFNEYDNWQASGVSGQG
                     ARSFYYPANSMMCADCHMPLQPSKDEGNINGFVHSHRFPGANTAVPTANGDAEQLAQS
                     VNFLQDKQVSVDVFAISPAGKEETSASPSAVASGQELSTTFAVGEEADVNAPKGATRE
                     ARAITAPLGRVNASVRRGEDYRVDVVVRTRKVGHFFPGGTVDAFDVWLELQATDDKGQ
                     LLYWSGKVEDDGKGPVEPGAHFYRSQQIDAHGNVINKRNAWSNRAVVYVHLIPPGAAD
                     TVHYRIHIPENAGEKINLHAKLNYRKFMWFNTQFSFAGQEDPKKAGGVFTPDYDDRKW
                     NFDADTSGVAGPMKRIPDLPIITVAEDTKSVAVLPKKDAEPAANTIAVKEDWVRWNDY
                     GIGLFLQGDLKAAEAAFTKVTEADAQNADGWVNIGRVRQLEGQTDAAAQVLDKALALK
                     PGLARANYFYARVLKEEGKYDEAIAKLQSVLQQYPRDRVVRNELGRIYFLQKKYADAV
                     KEFEVTLSIDPEDLQAHYNLMLCYNGLGNEAQAEAHKVRYLRFKADESAQAITGPYRQ
                     SHPEDNTERQAIHEHVSVALTAPSSASSAKKTTSVQKQLQSPIAASGKD"
BASE COUNT         1030 a         1664 c         1387 g         1199 t
ORIGIN      
        1 atagccctga ggaactgaat ttctctgccg ttttgggcag cgactgtttc gcaaacgttc
       61 gtgcactcgt ctggccctta ccctcggtca ccatcaacgt ctgaccggca ctcacgtcgc
      121 ttagcttgtc cacctggccg ctcggccacc ggatctcaac cgcatcggct ttgctccact
      181 ggccaagccc aaacgtcagc gccagctcgt tcgcggccag gtagctcgac ccgctccgca
      241 gcatctgcgt ctgcgttccg tccggagtcg tcagctttac caccgcgcca atcccgtcgc
      301 gattggactt ggtccccgtc agcttcaaac gcacgctgtg attcgcattt cccgcggcag
      361 tgtttcggaa cagatgcggt gctccagcgt tcgtcgaaaa caaaacgtcc agtcgcccat
      421 cgttgaacac atcggcgtac gcgacagacc ggcccacgcg cgccgaagca aattccgctc
      481 ccgccgattt cgtcacctcc gcgaattttc cctttcctaa attccggaaa aggtgtggcg
      541 gctccgcgaa cttcacgttc acctgcacct tctgaatgtc cgggtcgata tgaccattcg
      601 ccaccagaat gtccggccag ccgtccaggt cgtaatcaaa gaagaagcac ccaaatccca
      661 gcgtcagcag ggaagcgcgg cccacatcgc tgcgtggcgc ctcatccaca aacaatcctt
      721 tgccttcgtt gtgatacagc gaaatcatct ggttggaaaa attcgtgatc atcaggctgg
      781 tgttgccgct gcggtcgtag tcaaacgtat ccacgcccat ccccgcgcgc gccaccccgt
      841 cctcgctgaa cgccactcca gcaaccaccg ctttttctgt gaaggtcccg tttccgttgt
      901 tcttgtaaag cttgttcggt tgcgtgtcgt tggaaaaaag caggtccggc cagccgtcgt
      961 tgtcgtagtc cagcatcgcc actcccaacg ttttggaagt cggctcgccg agtcccgact
     1021 tctgcgtcgc gtcggaaaat ctcccgccac catcgttgtg ccacaaccgc accgaagttc
     1081 cccggtacga ttccggcgtg caataggatt tgtttttccc gtccatcgtg cagtacaagt
     1141 cgttttccgg cgaccactgc acataatttc caaccaccag atccagccgt ccgtctttgt
     1201 catagtccac ccaagccgcg ctggtgctga actctttcac tccgctcagt ccggcctttt
     1261 gcgtgacgtc ggaaaaagtt acgtttcccg ttttgtccgg accattgttg tggaacaagc
     1321 ggctctgccc gtaagcggtg acaaacaggt cgtcgtaccc gtcgttgtcg tagtcgccca
     1381 ccgcaacacc gatcccgtac atctcgacgt cgagtcccgc tttgcgcgtc acgtcggtga
     1441 atgttccgtc gtggttgttg tgataaagct ttggcgtcgt atgtttcgcg acgtgccccg
     1501 gccagtccgt tccattcacc agaaaaatgt ctggccaccc gtcgttgtcg tagtcaatga
     1561 atgccacgcc tggcccgatc gtttccggca aaaacttttt cccaaacgca ccattgttgt
     1621 gcacgaagcg aattccggcc ttcgccgtaa cgtccacgaa ttgcacgcca gcctgcgcgc
     1681 cttgcgcctt ggcgttcgcg ggaaaaagaa atgaaaaaca caacaaaact gaagaaatta
     1741 cggcggcaag tttgttcaag gcttctccgc agcgtcgccg cgcccgacgc ccagcgcatc
     1801 ggccacacga ttgatgtagc tgaaccatga agcgatcagc gtaatttgca aaatcccttt
     1861 atcgtcaaag cccacggcgc gcagcccgtc cagatcggcc ggtctgcact gcgtcgcgtc
     1921 tttcgtcaac ttcgccacgt aatcgcacat cacgcgttcc tgatcggtga tcggcgcaga
     1981 gtggtaatcg cgccgcagcg cctcaaccag cgaatcgtcc aacgtcaccc tacgcagaaa
     2041 ctctgcgtgc gattcaattc aataaacgca ccggttggtt acagaaacca tcgtcgcgat
     2101 catttcgtgc tgccggcgct gcagcggcag ttccggcgcc atcatcgcgc cgaacgcact
     2161 gaacgagtgg tacagcacat cgggcagcag cgtatgcgag cccacgatgc tgttctgaaa
     2221 accgcgatcc accgccggca ccggcgtggc gtactcctgc ggatacaatt tccgctccgc
     2281 ctcaacgaca gctttcactc gcgggtcgtc ttccattgca acagttttga tccaggtcat
     2341 cggctcggcc cttgggcaaa atttctttct gttttcggca aaagcaaatc agtcctttcc
     2401 gctggctgca atcggcgatt gcaattgctt ttggacactc gtagttttct ttgcagaact
     2461 cgccgagctc ggcgcagtca gcgctacgga aacgtgttcg tgaatcgcct gccgctcggt
     2521 gttgtcttcc ggatggctct gccggtacgg cccggtaatc gcctgagccg attcgtccgc
     2581 tttgaatcgc aggtagcgca ccttgtgagc ttcggcttgt gcttcattgc cgagcccgtt
     2641 gtagcaaagc atcaaattgt agtgcgcctg caaatcttcc ggatcgatgc tcagcgttac
     2701 ttcaaactct ttcaccgcgt cagcatattt cttctgcaag aagtaaatcc ggcccagttc
     2761 gttgcgcacc acgcggtcgc ggggatactg ctgcagcacg ctctgcagct tcgcaatcgc
     2821 ttcgtcgtac ttgccttctt ctttcagcac gcgcgcgtaa aaataattcg cgcgcgcgag
     2881 tcccggtttc agcgccagcg ccttgtccaa aacctgggcc gcggcatcgg tctggccttc
     2941 cagctgccgc actcggccga tatttaccca gccatcggca ttttgcgcat cggcttcggt
     3001 cacttttgta aacgcggctt cagcggcttt gagatcgccc tgcagaaaca atccgatgcc
     3061 gtagtcgttc cagcgcaccc aatcttcttt cactgcgatc gtatttgccg caggttcagc
     3121 gtctttcttc ggcagcacgg caacactctt ggtgtcttcc gcaacggtaa tgatcggcag
     3181 gtctggaatc ctcttcattg gcccagcgac tccggaagta tcggcgtcaa agttccactt
     3241 gcggtcgtcg tagtcgggag taaaaacgcc tccggccttc ttcggatcct cctgcccggc
     3301 aaatgagaac tgcgtgttga accacatgaa tttcctgtag ttcagcttgg cgtgcaggtt
     3361 gattttctcg cccgcatttt ccggaatgtg aatccggtag tgcacggtgt ccgctgcgcc
     3421 gggcggaatc agatgcacat aaaccaccgc gcgattcgac caggcgttgc gcttgttgat
     3481 gacgtttccg tgcgcgtcga tctgctgcga gcggtagaaa tgtgctcccg gctcgacggg
     3541 gcccttcccg tcgtcctcca cttttccgct ccaatacaag agctgtcctt tgtcgtcagt
     3601 ggcttgcagt tcgagccaca cgtcgaacgc atccaccgtt ccgccgggga agaagtggcc
     3661 gaccttgcgt gtacgaacta caacgtccac gcgataatct tcgccgcgcc gcaccgaagc
     3721 attcacgcgg ccgagcggtg cggtgatcgc gcgggcttcg cgcgtcgctc cttttggagc
     3781 attcacatcg gcttcttcgc cgacggcgaa agttgtggac agttcctggc cgctagcgac
     3841 agcggaaggc gacgccgaag tttcttcttt gccagccggc gaaatcgcga acacgtccac
     3901 gctgacttgc ttgtcttgta ggaagttcac gctttgcgcc aattgttccg cgtcgccgtt
     3961 tgctgtcggc accgctgtgt tcgcgcccgg aaagcggtgt gagtgtacga agccgttgat
     4021 gttgccttcg tctttcgacg gttgcagcgg catgtggcaa tccgcgcaca tcatcgaatt
     4081 tgccggatag taaaacgacc gcgcgccttg cccgcttacg ccgctggctt gccagttgtc
     4141 gtattcattg aagccgcgca cccagcgata acgattcacc ggcacgtcga gatgcacttt
     4201 gtggcagccg gaacagaatt ccgccgtctg ctcgcgcatg aacggtttca ggaaagttcg
     4261 gcggtgcggt tcgggattca ctttcaccat gaagtcgtgc aacgtgcgca tcacgggatt
     4321 ttcgctggcg gccagttcgt gcagtttggg gtattcgagc acgaaatcgc cctggcccat
     4381 ggtgcttttg actttcacga tgctgtggca catcatgcaa ctcaagccgg cctggccagc
     4441 gggcgtgttt tcgatctgcc ggatcggcgt atcgaacatt ccactgaaca aaagcgcggg
     4501 gtcgtggcaa ccggcgcacc acttggaagc cttcacgccg gcgacgtcct gcatgtactc
     4561 gatacttttg cgataccact gattgttgaa cgaagaaaaa tgatgggccg agctgtacca
     4621 ctgcttgtac acatcgttgt ggcagcgttc gcaggcctgc gactgcatga aatacttcgc
     4681 cggaatgttt ttaccgtcgc gggtctcgac cgaactcggg aagaacttgc cgttcgggcc
     4741 gtcgccttca ccgtccatgg tttcagcggg cattttggga ttcaccacgc ggtaggcgtt
     4801 tttccaagcg acagcgcgcg tccaccaagc gccggcgctc gcgcctgtga agagtaacgc
     4861 aacgacggca aagcttgccg cggctccagc gaatccttta ccgagccagc cgcgccgtgc
     4921 gatccacgcg ctcgccagga gcaaaacgcc cgccgcacac agcgcgatat gcaggtacaa
     4981 ccaggtcttg aaacggttcg gcgtaccgat cttgatcagc gcgagcccga gaatcgtccc
     5041 cgccgtcagc agcacccagc caaatttctc gccaacggag cattcccgaa agacggcgcg
     5101 taaaatcgga atcagcagca ccgccaacaa aatgccagcg cccgcatgca gcaagactcc
     5161 gccaccgtag atgatcgtcg cggcaggaaa cgtatagaga tagatcgcgg aaagtgtcag
     5221 gaccaggacc aggatcgacc gccagcggct cattgctcgc ttaccccaac taacattttg
//
LOCUS       DASTPM010000212         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_641553, whole genome shotgun sequence.
ACCESSION   DASTPM010000212 DASTPM010000000
VERSION     DASTPM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641553"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /gene="lptF"
                     /locus_tag="VFR42_03740"
     CDS             complement(<1..1196)
                     /gene="lptF"
                     /locus_tag="VFR42_03740"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEU0368295.1"
                     /translation="MRILDRYIVREIFRHAFLGLIVFTFVLFVPQLVRLMELLVRHSG
                     SGAQIARLFLYVVPGVLIFTIPMAVLVGVLLGLGRMSADSEIIALTALGISRQRMLVP
                     VAVLALTGGVATATLTLWLGPAAFRSLRNIEADLLASQASFQVQPRVFDERFPKMVLY
                     INDISASGTQWHGVFLAEVGEESGSQLTLAENAIVIAEPKQGKLELHLQGGTTHEVSR
                     QDPDHYSLTDFGQSDMPIEVSGLVPTQQRALSNAERRTLDLLSDRSANWRETRVEFHR
                     RLAFPFACLVFALVAVPLGSQPRRGGRAAGTILSVLLIGLYYSLFITGAGLSREGKLS
                     AGLGIWLANIALTALGLALLPRMEQLGSDSKWLRRFTRLDLWKRLLRRKKVQARLKAA
                     EAARTG"
     gene            1253..3454
                     /locus_tag="VFR42_03745"
     CDS             1253..3454
                     /locus_tag="VFR42_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HEU0368296.1"
                     /translation="MNKVHLVLLWHMHQPQYRDPETGRYVLPWTRLHALKDYWGMVKI
                     LEDFPKFHATFNMVPSLCMQLEEYASGKFNEPWFELAFRDAEQLSREEKAEILQRAFQ
                     VNHERLLARWPRFLELYEWSRPAGGAQAQVTFTPRDWRDLQLLSQLVWMDEEWIAKDP
                     LISPLCAKGKDFSEKDKATLKKKQLEYLQIVLPIYREAASRGQIEISTTPFYHPILPL
                     ICDSDIARIANPSTLVPRRPYRHPEDAREQLRRARECHQRIFGVLPSGLWPSEGSVSD
                     QTLAIAAEEGFRWFGTDEGVLGRTLNVGFFREASGLPANADKLYQPWRVQMGSNGITG
                     LFRDHHISDLIGFVYSRMDSKAAAADLHGRLRLIGDRVPSKQPLTVCIFLDGENAWEY
                     YPGHGREFLREFYRRIQDDSDFRALSATEAIAAAGEIPQIGGIFPASWINANFDVWIG
                     HGEDIQAWELLWDAREAYGKAEEARTQGRSDAPSEVALKQAKESLLAAEGSDWCWWYG
                     PEHSTANDAEFDALYRKHLTGIYRALGVVAPDELAKPIKRQPERALHLTPSEYLRIDV
                     NGRDSSYFEWLGAALYSPERRSGAMHGRTYYLRELRYGFEPERFCVRLDAFPEALAEL
                     EEAEFRVSVHAQCGVTITAKLKHGKLVEFSVEQERVCLLNPSGVATVAFETILEVAIH
                     KDLFDLRGATRLRLGVALWHGGLPVDVLPAEGILDVQLGEDNFAWPLEPLS"
     gene            complement(3496..>4405)
                     /locus_tag="VFR42_03750"
     CDS             complement(3496..>4405)
                     /locus_tag="VFR42_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HEU0368297.1"
                     /translation="PTRAEVWGVSAVGGFAWVLDSRTSKILARIPVGALPYSLDFSPD
                     GARVYVPSSGGNLLSAIDCVSHQIVGQAPTGAQPVAARISSDGKLALVLNKKDATVSV
                     YDAATLRLLAVVPVIPDPEDAAILPDNSVAFILSRSQKRLSVIDLRKGLLLTNLELAG
                     VPSQMILKADGGELYVLSPEAHGLQAINTWTHEVGDYVELGSAPTRGVLLPDTGQMYV
                     SDAETNMVTGVDLNYRRLIRPSVPAGRIPGALRFDPGDRARLLLAVSQASGDLSIIRV
                     TSDRPSGLLTMIPVGDQPRELAVKLF"
BASE COUNT          925 a         1323 c         1315 g          842 t
ORIGIN      
        1 ttgcctgtgc gcgccgcctc ggctgccttt agccgcgctt ggactttttt gcggcgcagg
       61 agacgcttcc acaggtctag cctagtgaag cgtctaagcc acttgctgtc gctgcctagt
      121 tgttccatgc gcggcagcaa cgccagtccc agcgcagtca acgcgatgtt ggccagccag
      181 attcccagtc cagcactcag tttgccttcg cgtgaaagcc ccgcgcccgt gatgaacaac
      241 gagtagtaca gcccgatcag cagcaccgaa agaatcgtac cggccgcgcg accaccgcgc
      301 cgcggttgcg aaccaagcgg taccgccacc agcgcaaaca ccaggcacgc aaaggggaac
      361 gccagccggc ggtgaaactc cacgcgcgtt tcgcgccagt tcgcgctgcg gtcagacaac
      421 aaatccaagg tgcggcgctc ggcattactc agcgccctct gctgcgttgg caccaggccg
      481 cttacttcaa tcggcatgtc gctctggcca aagtccgtca gcgaataatg gtcgggatcc
      541 tgccggctca cctcgtgtgt tgttccgccc tgtaagtgca gctccagctt gccctgcttc
      601 ggttcggcaa ttacaatcgc attctctgcc agcgtcagct gtgagccact ttcttcgccg
      661 acctctgcga ggaacactcc gtgccactgc gttccgcttg cggaaatgtc gttgatgtac
      721 agcaccatct tggggaagcg ctcgtcgaac acacggggct gcacttgaaa cgacgcctgc
      781 gaagccagca ggtcggcctc gatgttgcgg agcgaacgga acgcggctgg cccgagccat
      841 agcgtgagtg ttgcggtggc gacgccgccg gtcagcgcca gtaccgctac cggcaccagc
      901 atgcgctgcc ggctaatacc cagcgcggtc agtgcgatga tttcgctgtc ggcggacatg
      961 cggcccagtc ccagcagcac gccgaccagc acggccatgg gaattgtgaa gatcagcacg
     1021 ccggggacga cgtacaagaa gagtcttgcg atctgcgcgc cggaacccga atggcgcacc
     1081 aggagctcca tgagccttac cagttgcggc acaaagagca cgaacgtgaa gacaatcagt
     1141 ccgaggaagg catgacggaa aatttcgcgg acgatataac ggtcaagaat gcgcatggcg
     1201 cgattcgtga gtgtagcatg ctacctgccg cgttgcggga gcgctgccgg caatgaacaa
     1261 agtccatctg gtcctgctct ggcacatgca ccagccgcag tatcgcgacc cggaaaccgg
     1321 ccgttatgtc ttgccgtgga cccggctcca cgccttaaaa gactactggg gcatggtgaa
     1381 aatcctggaa gattttccga agttccatgc caccttcaac atggttccct cgttgtgcat
     1441 gcagctggag gaatacgcca gcggaaaatt taacgaaccg tggttcgaac tggccttccg
     1501 cgacgcggag cagctgagtc gagaagaaaa ggccgaaatc ttgcagcgcg cttttcaggt
     1561 caatcacgag cgcctgttgg cgcgctggcc gcgcttcctg gagttgtacg aatggtcgcg
     1621 accggctggt ggcgcgcaag cgcaggtgac atttaccccg cgtgactggc gcgacctgca
     1681 acttctttcc cagctggtgt ggatggacga agagtggatc gccaaagatc ccctcatcag
     1741 ccccctttgc gccaaaggaa aagacttcag tgaaaaagac aaggccacgc tgaagaaaaa
     1801 gcagcttgaa tatcttcaga ttgttctgcc catttatcgc gaagcggcca gccgcgggca
     1861 gatcgaaatc agcaccacgc cgttctacca cccgattttg ccgctcatct gtgattcgga
     1921 catcgcgcgc atcgcaaatc cttccaccct ggtgccgcgc cggccctatc gccatccgga
     1981 agacgcccgc gaacaactgc gccgggcgcg cgaatgccac caacgcattt tcggcgtgct
     2041 gccttccggg ttgtggccgt cggaaggttc cgtctccgat cagactcttg cgatcgcggc
     2101 agaggaaggt tttcgctggt tcggaaccga cgaaggcgta cttggccgaa cgctgaacgt
     2161 tggatttttc cgcgaagcga gcggcctgcc tgcgaacgcc gacaagctct atcaaccgtg
     2221 gcgcgtgcag atgggttcga acggcatcac tggactcttc cgtgaccatc acatttccga
     2281 tttgatcggc tttgtctaca gccgcatgga ttcgaaagcc gctgccgcgg acctgcacgg
     2341 acgcctgcgc ctcatcggcg atcgtgtgcc gagcaaacag ccgctcaccg tctgcatttt
     2401 tctggatggt gaaaacgcct gggaatatta tcccggccac gggcgcgaat ttttacgcga
     2461 attctatcgc cgcatccagg atgattctga ttttcgcgcg ctctccgcga ccgaagcgat
     2521 cgccgcggct ggcgagattc cgcagatcgg cggaattttt cctgcttcct ggatcaatgc
     2581 caattttgac gtgtggatcg gccacggcga agacatacaa gcgtgggagt tgctctggga
     2641 tgctcgcgag gcctacggca aagcggaaga agcgcgcaca cagggacgca gcgatgcgcc
     2701 cagcgaagtg gccttgaaac aagccaagga gtctttgctg gcggctgaag gtagcgactg
     2761 gtgctggtgg tatggccccg aacacagcac cgcgaacgac gcggaattcg acgcactgta
     2821 tcgcaaacac ctcactggta tctaccgcgc tctcggcgtg gtcgctccgg acgaactggc
     2881 caagcccatc aaacgccagc ccgaacgcgc actgcatctg acgccttctg aatatctccg
     2941 gatcgacgtg aatggtcgcg actcttctta tttcgaatgg ctgggcgcgg cgctgtattc
     3001 gccggagcgg cgcagcgggg ccatgcacgg tcgcacatac tacttgcgcg aactgcgcta
     3061 cggttttgaa ccggaacgtt tttgcgtccg gctggatgca tttcccgaag cgctggccga
     3121 actggaagaa gccgagtttc gcgtgagcgt ccatgcgcaa tgcggagtca ccatcacggc
     3181 caagctgaaa catggcaaac tggtggaatt ttcggtggag caggaacgcg tctgcctgct
     3241 caacccatcc ggagtcgcca ccgtcgcgtt cgagaccatc ctcgaagtgg ccattcacaa
     3301 agaccttttc gatttgcgcg gtgcgacccg cttgcgacta ggcgtagccc tgtggcatgg
     3361 tgggctgccc gtggacgtgc tgcccgcgga agggattttg gatgtgcaac tcggagagga
     3421 taactttgcc tggccgctgg agccgctctc gtagttaaaa ccacagccga gccgagacgt
     3481 agtccgctag gcgccctaga acaatttgac ggcaagctcg cgcggttgat cgcctacggg
     3541 aatcatcgtg agcaagccgc tcggacgatc cgaagttact cgaatgatgg agagatcgcc
     3601 ggacgcttgg ctgaccgcca gcaacaagcg cgctctgtcg cctggatcaa aacgcaacgc
     3661 tccaggaatc cgtccggcgg gaaccgaagg gcgaatcagg cggcggtaat tcaagtctac
     3721 gccggtcacc atgttggttt cggcatcgga gacatacatc tggccggtat cgggcaagag
     3781 tacgccgcgg gtcggcgccg agcctaattc aacatagtcg ccaacttcgt gcgtccacgt
     3841 attgatggct tgcaagccat gcgcttcggg agaaagcaca tagagttcgc caccatcggc
     3901 tttcaaaatc atttgtgaag gcacgccggc gagttccaga ttggtcagca acaggccttt
     3961 gcggagatcg atcaccgaca agcgtttttg cgagcggctg agaataaagg ccaccgagtt
     4021 gtccggcaag atcgcggcgt cttcgggatc cggaatcacg gggactaccg ccagcagccg
     4081 caaggttgcc gcgtcataga cgcttaccgt ggcgtctttt ttgttcaaaa ccagtgccag
     4141 ctttccatcg gacgaaatgc gcgccgcgac gggctgcgcg ccggtcggag cctgcccgac
     4201 gatttggtga gacacgcagt cgattgcgga aagaaggttg cctccggatg agggaacgta
     4261 aacgcgggcg ccgtcgggag aaaaatccag agaataggga agcgcgccaa ccggaatgcg
     4321 ggccaggatt ttggatgtgc gcgaatccag aacccaagcg aagccgccta cggcgctaac
     4381 gccccagacc tcggcacggg tcggg
//
LOCUS       DASTPM010000213         5163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_266931, whole genome shotgun sequence.
ACCESSION   DASTPM010000213 DASTPM010000000
VERSION     DASTPM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266931"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="VFR42_03755"
     CDS             complement(<1..1633)
                     /locus_tag="VFR42_03755"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0368298.1"
                     /translation="MNRIRKWWKPLVAVVLAALALQFGASLLVRTTRLRLFLTRQLER
                     SFGRPVDVREFSASLFPTPQLDAYAVSVGEDPAFGNEYFLRADRLSASLRWWGLLRGR
                     FELGTLQLNRPSLVLVRNAEAQWNLERWLPAAGFEAKPTMGLALKAGLPINHLQKIEI
                     NDGRVNFKIADDKASFAFQQVEGTMEQTAAGRWRLNLEAEPWRSGVPLQLAGTMRVSG
                     DVAGTSVRLRPAHFRVSWTKSSLADVFRLIGGSDFGVRGTFSGDATVDSDENSLPETK
                     DATLGDWAFSAQLRATEIHRWDLSERGDNPRLAFRIKGRWNPALGITQASEVVLETPR
                     SNVRGTAALQSASELSLEVRLDSAGIQAADFLDWYRAFQPDVAEGIAASQYFTGTASL
                     SGWPLRLREAAFSSPGGRWTVPGFASVVNVRPIRGGTQRGRLITEPFTISLPGKKHGE
                     SDRRTTLTTAKDTTGNSTVGTITVTFQDDLAQHSGSLRLEGQLQQVETVLAAASAFGH
                     DLRHGWDLSGKCAGDLRLEWSRNHPALWNGRADLTR"
     gene            complement(1732..2544)
                     /gene="lepB"
                     /locus_tag="VFR42_03760"
     CDS             complement(1732..2544)
                     /gene="lepB"
                     /locus_tag="VFR42_03760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0368299.1"
                     /translation="MSEPSTVAQVDAAEPPPIAEHTAHRQATTLSEYMESLLVTVILA
                     LFGTSFVVQAFKIPSSSMERTLLIGDHLLVNKFIFGGTGAWYERFLPYRPLQRGDIIV
                     FKYPYADHQHFVKRVIGLPGDRLKLVDQQVYINGKTLNEPYVVHDAAAGYDPLNYSFP
                     PLGNPLYSSPVEPEWARDIRKHIKGDELIVPPDKYFAMGDNRDHSLDSRYWGFVDRDA
                     IMGRPFLIYWSVEATSADYGESTFTQRLRGVLVTIAHLPARTRWSRMLHTVH"
     gene            complement(2541..3293)
                     /gene="rnc"
                     /locus_tag="VFR42_03765"
     CDS             complement(2541..3293)
                     /gene="rnc"
                     /locus_tag="VFR42_03765"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU0368300.1"
                     /translation="MTRLEDDELQERLGYEFAKPSLLVTALTHSSAIPELRTAAESAG
                     TDAAEIQDNERLEFLGDAVLDLLASEYLLESFPSWSEGQLSRSRAYLVNAQALEIVAR
                     KIGIGEFLRLGRGEEKTGGREKAAIIADALEAVVAAIYLDSGLEAARRVLHRVLFESA
                     LELGGVRIADSDRKSALQEFLQGKGKPPAEYRLAGETGPDHQKSFYVEVWSLGELLAS
                     GQGYTKKEAEQKAARMALERLQQQADVSTKAE"
     gene            complement(3327..4193)
                     /locus_tag="VFR42_03770"
     CDS             complement(3327..4193)
                     /locus_tag="VFR42_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368301.1"
                     /translation="MSGRKSWVTAVFVAAGLWALGAPAFAQGQSGQPGQSQPGQQTAD
                     PSKSGSSLAVPQAPQNAEELAAVKVFQEMPNTDLPKKIAAGEEFLNKYPNSRYRPMVY
                     SALTLEYIQAGNTDKAFETGDKEIALKPDDVQTLAVLGQTIPRVMNASTPEPEKQLAK
                     AEDYSKRAIQVTPTIPKPEGMPDQNFVAAKNSTLAMAHSGLGLVYFRRGKFSDAIPEL
                     EQSVKIDPNPTPDPVNLYLLGLANQKASHYDDAAVAFNKCASLNSKLQETCRNGADQS
                     KKQASTQLSAPK"
     gene            complement(4282..>5163)
                     /locus_tag="VFR42_03775"
     CDS             complement(4282..>5163)
                     /locus_tag="VFR42_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368302.1"
                     /translation="QLDTVRHAFLHYLLDPLPLQYSHVIAVKRPLFEMAAAAPRLDPT
                     LKDDFPSYFAECTVRAVDLKLKRMSPGEREAALGVDDADGYVLVRPLFAALTKFEQSE
                     PAMKLYFPDLVRSVDVGTEQKRLAAITFATGRPTSEAAEAESVARHKTVAPSTVPNDA
                     DAIAALTEGERRIAEKNPRAAEASFQKVLMKYPDQPRAWYGLGVVAMMEQNGPRAKEV
                     FGRLTEGEHAAMQDPLVLAWSHVHLGNLYDIEGQPDRAKSEFEAALSVQGAPERAKEA
                     ATKGLSTISTEKKPERP"
BASE COUNT         1047 a         1561 c         1398 g         1157 t
ORIGIN      
        1 cacgggtcag gtcggcacga ccgttccaaa gggcgggatg atttcgactc cattccagtc
       61 gcaagtcgcc cgcgcacttg cccgacaaat cccaaccatg ccttaggtca tgcccgaacg
      121 ccgatgcggc ggcaagaacc gtttccacct gttgcagctg gccctcaagg cgcaaactgc
      181 ccgaatgctg tgcgagatcg tcctggaaag tgacagtaat ggtgcctacc gtggaattgc
      241 cggtggtgtc cttggcggtg gtcaaagtcg ttctgcggtc agactctcca tgtttttttc
      301 cgggcaggct gattgtaaaa ggttcggtta ttagccttcc gcgctgagtt ccgccgcgaa
      361 taggtcgaac gttcacaacc gacgcaaatc ccggcacagt ccagcggcct ccgggactcg
      421 aaaacgctgc ctcccgcagt cgcaacggcc acccgcttag gctagccgtg ccggtgaaat
      481 actgggacgc agcgattcct tcggctacgt cgggctgaaa tgcccggtac caatccagaa
      541 agtctgcggc ctgaatgccg gcggagtcca gacggacctc aaggctcaac tctgacgcgc
      601 tctgcagtgc ggcagttcca cgcacgttgg agcggggagt ctccagcaca acttcgctag
      661 cttgggtaat tcccaaggca ggattccagc gacccttgat gcgaaatgcg agtcgaggat
      721 tgtctccacg ttcagacaga tcccagcggt gaatttctgt tgcccggagc tgggcgctaa
      781 aggcccagtc gccgagtgtg gcgtccttgg tttcgggtaa tgaattttca tcgctgtcaa
      841 cggtggcgtc tccggaaaac gtgccgcgca cgccgaagtc gcttcctccg atcaatcgaa
      901 atacatccgc cagcgaggac ttggtccaac tcacgcggaa gtgcgctggg cgcaggcgga
      961 ccgacgtgcc cgcgacatct ccgctcacac gcatcgtacc agccaattgt agcggaaccc
     1021 cgctgcgcca cggttccgct tccaagttga gccgccagcg acccgcagcg gtctgttcca
     1081 tggtgccttc gacttgctgg aatgcaaacg aagctttgtc gtcggcaatc ttgaagttga
     1141 cacggccatc gttgatctca atcttttgca gatgatttat cggcagcccc gccttcagag
     1201 cgaggcccat ggtcggtttc gcttcaaagc ccgcggccgg cagccaccgc tcgaggttcc
     1261 actgcgcttc cgcatttcgc accaggacca agcttgggcg attgagttgc agcgttccta
     1321 gttcgaatcg accgcgcagt aacccccacc agcgcaaact agcggaaaga cgatccgcgc
     1381 gcaggaagta ttcgttgccg aaagctgggt cttcgcctac ggaaaccgcg taagcgtcca
     1441 actgcggcgt gggaaaaagg ctcgctgaaa actcacgcac atctaccggg cggccaaagg
     1501 agcgctccaa ctgacgcgtc agaaacaatc tgaggcgtgt cgtacggacc agcagcgagg
     1561 cgccaaattg cagcgccagc gcagccagca cgactgcgac tagaggcttc caccattttc
     1621 tgattcgatt cactgaattc gattcggaac ggacagcgcc gttcgatgcc aaaaaattct
     1681 cgcagacttg gtggcgaatt ggtagcaggc taaacgagtt tcagcaagag gctagtgaac
     1741 ggtgtgcagc atgcggctcc agcgcgtgcg cgccggcaag tgtgcaatcg tcaccaacac
     1801 tccgcgaagc ctctgcgtaa aagtggactc gccatagtct gctgaagttg cctccacaga
     1861 ccaataaatc aggaacggac gacccatgat cgcgtcccgg tccacaaatc cccaataccg
     1921 gctgtcgaga ctatggtcac ggttatcgcc catcgcgaaa tacttgtcgg ggggaacgat
     1981 caactcatcc cctttgatgt gctttctgat atcgcgagcc cattctggtt ccaccggcga
     2041 actgtacaga ggattcccta gcggtggaaa ggaatagttc agcgggtcat aacctgctgc
     2101 cgcgtcgtgc accacgtatg gctcgttgag cgtctttccg ttgatgtaaa cctgttggtc
     2161 caccaatttg agacgatcgc ctggcaaacc aatcacacgc ttcacaaaat gctgatgatc
     2221 ggcgtaggga tatttgaaaa cgatgatgtc accacgttgc agcggccggt agggcaagaa
     2281 cctttcatac caggctcccg tcccgccgaa gatgaatttg ttcaccagca ggtggtcacc
     2341 tattaaaaga gttcgttcca tcgaggacga ggggattttg aaagcctgca caacgaaact
     2401 ggtgccaaag agcgccagaa tcactgtgac cagcagcgat tccatgtatt cggaaagcgt
     2461 cgtggcctgg cgatgcgcgg tatgctccgc aatcggaggc ggttcggcgg catcaacttg
     2521 agcaactgtg cttggttcgc tcattcggct ttggtggaca cgtcggcctg ctgttgtaag
     2581 cgctccagcg ccatgcgcgc cgccttctgt tccgcctctt ttttcgtata gccttggccg
     2641 ctcgctagaa gctctccgag cgaccacact tctacataaa acgatttctg gtgatcggga
     2701 ccggtctcac cggcgaggcg atactcggcg ggcggcttgc cctttccctg cagaaattcc
     2761 tgcagagccg atttgcggtc cgaatccgca atgcgaactc cgcccaactc tagtgcggat
     2821 tcgaacagca cgcggtgaag cacacgcctc gctgcctcca acccggaatc gagatagatc
     2881 gcggcaacta ctgcttccag cgcatccgcg atgatcgcgg ctttctcgcg tccgcccgtc
     2941 ttttcttcac ctcgacccag ccgcaagaat tcgccgatgc cgatctttcg cgcaacaatt
     3001 tcaagtgctt gcgcgtttac aagatacgcc cgactccgcg acagctgccc ttcgctccac
     3061 gatggaaaac tttccagcaa atactcactc gccaacagat ctaggaccgc gtcgcccaaa
     3121 aattcaagac gttcgttgtc ctgaatttcc gctgcatcag ttcccgctga ctcggccgcc
     3181 gtacgcagtt caggtatggc ggaactgtga gtcagcgccg taaccagtag gctaggtttg
     3241 gcgaattcat atccgagtcg ttcctgcagt tcgtcgtcct cgagacgggt catcttttac
     3301 cgccataata catcccggca gcaatgttat ttcggcgcgc tcagttgtgt cgaggcctgt
     3361 ttcttggatt gatcagcacc gtttcggcat gtctcttgca gtttgctgtt gagcgaagcg
     3421 catttattga acgccacagc cgcgtcgtca taatgcgagg ctttttggtt cgcaagcccc
     3481 agcaagtaca aattcaccgg gtcgggggtt ggattcggat cgatttttac agactgttct
     3541 agctccggaa tcgcatcact aaattttccg cgccgaaaat acactaagcc gagaccgcta
     3601 tgcgccatgg ccaatgttga attcttggcg gcaacaaagt tttgatccgg cattccttct
     3661 ggcttgggaa tggtaggggt cacctgaatg gcccgcttcg aatagtcttc cgccttggcg
     3721 agctgttttt ccggctcggg cgtgctggcg ttcatgactc gcgggatagt ttgcccgagc
     3781 actgccaggg tctgcacgtc gtccggcttt agcgcgatct ctttgtcgcc agtttcgaat
     3841 gccttgtcag tattgcctgc ctggatgtat tcaagtgtca atgccgagta gaccatcgga
     3901 cggtaccggc tatttggata tttgttcagg aattcttcgc ctgccgcgat tttcttgggc
     3961 agatccgtgt taggcatttc ttgaaacacc ttcaccgccg cgagctcctc cgcgttttgc
     4021 ggagcttgtg gcacggcgag tgaacttccg gacttgctag gatccgcggt ctgctggccg
     4081 ggttgacttt gtccaggttg gccggattgg ccctgcgcga atgccggcgc gcccaacgcc
     4141 cacagccccg ccgcgacgaa cacggcggtg acccaactct tcctaccgct cattttgctt
     4201 ctccttgctt tggaatcgtc tgcggctggt gtgatcttca tgatacatct ctgcgtacca
     4261 tcggtacgta cagcatttcg cctaaggccg ttccggcttt ttctccgtgc tgatcgtact
     4321 caaacctttt gtagccgctt ccttcgccct ttcgggcgct ccctggacgg aaagcgctgc
     4381 ctcaaattca gacttggcgc gatcaggctg accttctatg tcgtagaggt tacccaaatg
     4441 aacgtgcgac catgcgagaa cgagcggatc ctgcatcgcc gcgtgttctc cctcggtcag
     4501 ccgaccgaag acttccttcg cgcgagggcc gttctgctcc atcatggcca caactccgag
     4561 cccgtaccac gcacgcggtt gatcgggata tttcatcagc actttctgaa acgacgcttc
     4621 ggccgcgcgc gggttctttt ccgcgattcg ccgctcgcct tccgtcagcg ccgcaatcgc
     4681 atcggcgtcg ttcggcaccg tagaaggcgc aaccgttttg tgtctggcaa ccgactcggc
     4741 ctcggcggcc tcggaggtcg ggcggccggt cgcaaacgta atagcggcta gtcgtttttg
     4801 ctcggtacca acgtccaccg atcgcaccaa atccggaaaa tacaatttca tcgctggctc
     4861 ggactgttcg aactttgtca gtgccgcaaa cagtggccgc accaaaacat agccgtcggc
     4921 atcgtcgacg cccaatgccg cttcccgttc gcccggcgac attcgcttca gcttcaaatc
     4981 tacagcccgc accgtgcact cggcaaagta cgaaggaaaa tcatccttca gcgttgggtc
     5041 cagacgcggt gccgccgccg ccatttcgaa cagcggccgc ttcaccgcga taacgtggga
     5101 atactgcaac ggcaacgggt ccagcaaata atgcagaaac gcgtgtcgca ccgtatccag
     5161 ctg
//
LOCUS       DASTPM010000214         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1275871, whole genome shotgun sequence.
ACCESSION   DASTPM010000214 DASTPM010000000
VERSION     DASTPM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275871"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1124
                     /locus_tag="VFR42_03780"
     CDS             66..1124
                     /locus_tag="VFR42_03780"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0368303.1"
                     /translation="MKRKLLVAVPLLCLVVVAAWYVRPKHEYLGEAYVSERSTTLWSS
                     VAQVREPIDLLHYGDRVEVLARRNDSAKVRTTSGAIGWVDARLLLEPALWQRSVKLLS
                     AAKVMPVQARGRTKVQTNLRVEPGRTQPRLYQFGRGVPVEVVARAVSDWVQVSDEREN
                     NEAQATKKEDWFLVRGLATRPPGEASARSAESNTTTQPGDQTVPIAGWVVARFVELDL
                     PEPVREGMSSANVRPISWFELNRVDSPSGAKPQYLVAGTRSPEGQSCDFTTLRVYTWN
                     QKRTRYETAFIENNLCGQMPIRLDIGSKGEPQFRFTQMQGAKEERLYRLTQTVVRRVR
                     EPGEPVHKSAPTTKAAKP"
     gene            1316..2266
                     /locus_tag="VFR42_03785"
     CDS             1316..2266
                     /locus_tag="VFR42_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368304.1"
                     /translation="MSASLLLSACSHPTQTVAAQQPPPPPFEYVDTWGSHGDGPGQFS
                     KPIAMASDGESIIYIADAATGFVHKFAPSGEPRLSFQDDRTDMHPVDLAVDAGAAIYV
                     ADGRRATVAIFFSDGMRHRELRTNAISADRQSLRIAVDSYGTIYATAKSPYGVRKFST
                     ALRHLGTWAAPAPKVAKAAKNSSPAASPDNLIDDPSAIAVGPDGLIYVSESAQPQIKV
                     YDSAGAPQRTLTVPAEVTDAQLTGLAINKKFIFAVSATRPSIYIWSLDGNYRMTQDLS
                     TWVPGTGSAITRKIAVTPANDLLLLDTAAARIFRFRLHLQ"
BASE COUNT          450 a          929 c          567 g          390 t
ORIGIN      
        1 cgtgtcgcgc aaaaaatgat caccccttca ctccgtgtgc catccgccga gcccgaggcg
       61 cgcccatgaa gcgcaagctt ctcgtcgccg tcccgctgct ctgcctcgtc gtcgtcgccg
      121 cctggtacgt gcgccccaag cacgaatacc tcggcgaggc ctacgtcagc gagcgcagca
      181 ccacgctgtg gagcagcgtc gcgcaagtcc gcgagcccat cgacctcttg cactacggcg
      241 atcgcgtcga agtcctcgcg cgccgcaacg acagcgccaa agtccgcacc accagcggcg
      301 ccatcggctg ggtcgatgcc cgcctgctcc tcgagcccgc gctctggcag cgcagcgtca
      361 agctgctctc cgccgccaaa gtcatgccgg tgcaagcccg cggccgcacc aaagtgcaaa
      421 ccaatcttcg cgtcgagccc ggccgcaccc agccgcgcct ctatcaattt ggccgcggcg
      481 ttcccgtcga agtcgtcgcg cgcgccgtct ctgattgggt gcaggtcagc gacgagcgcg
      541 aaaacaacga agcgcaagcc accaaaaaag aagactggtt cctggttcgc ggcctggcca
      601 cgcgcccgcc cggcgaagct tccgcgcgca gcgccgaatc caacaccacc acgcagcccg
      661 gcgatcagac cgttcccatc gccggttggg tcgtcgcccg tttcgtcgag ctcgatttgc
      721 ccgagccggt ccgcgaaggc atgtcctccg ccaacgtccg ccccatttcc tggttcgagc
      781 tcaatcgagt cgatagccct tccggcgcaa agccgcaata cctggtcgcc ggaacgcgca
      841 gcccggaggg ccagtcctgc gatttcacca ccctccgcgt ctatacctgg aatcagaagc
      901 gcactcgcta cgaaaccgcc ttcatcgaaa ataatctctg cggccagatg cctatccgcc
      961 tcgacatcgg ctccaagggc gaaccccaat ttcgtttcac ccaaatgcaa ggcgccaaag
     1021 aagagcgcct ctatcgcctg acgcaaaccg tagtccgccg cgtccgcgaa cccggcgaac
     1081 cagttcataa atccgcgccg accacgaaag ccgcaaaacc ctagtgcgac ctccatttcg
     1141 ccatcacgca cgccatcatc aagcactccg tcatcaagga cactgccatc acgcactctg
     1201 tcatcctgag cgtacccgac aagggtacgc gaaggacctc aacgtaatgc cctctcaaga
     1261 cgtaaaccga gcaactcgca ccgcgacccc agcgcccgca atttttttga ttgcaatgag
     1321 cgcatcactg ctcctgtccg cctgttccca ccccacgcaa accgtcgccg cccagcaacc
     1381 ccctccgcct cccttcgagt acgtcgacac ctggggctcc cacggcgatg gccccggcca
     1441 attctccaag cccatcgcca tggccagcga tggcgaatcc atcatctata tcgccgacgc
     1501 cgccaccggc ttcgttcaca aatttgcgcc tagcggcgaa ccgcgcctct ccttccagga
     1561 cgatcgcacc gacatgcacc cggtcgacct cgccgtcgat gccggcgccg ccatctacgt
     1621 cgccgacggc cgccgcgcca ccgtcgccat cttcttctcc gacggtatgc gccatcgcga
     1681 gctccgcacc aatgccatct ccgccgaccg ccaatctctg cgaatcgccg ttgattccta
     1741 cggcaccatt tacgccaccg ccaagagccc ctacggcgtc cgcaaattca gtaccgcgct
     1801 gcgccacctc ggcacctggg ccgcacctgc gccgaaagtt gcgaaggccg caaagaattc
     1861 ttcgcccgcc gcgtcaccag acaacctgat cgacgatccc tccgccattg ccgtcggccc
     1921 cgacggcttg atctacgtca gcgaatcggc gcaaccgcaa atcaaggtct acgattctgc
     1981 cggcgccccg caacgaacgc tcaccgtccc cgccgaagtc accgacgccc agctcaccgg
     2041 cctcgccatc aacaagaaat tcatcttcgc cgtcagcgcc actcgtccct ccatctacat
     2101 ctggtccctc gatggcaatt accgcatgac ccaggatctc tccacctggg ttcccggcac
     2161 cggctccgca atcacccgca aaatcgcggt aacccccgcc aacgacctcc tcctcctcga
     2221 caccgccgcc gcccgcatct tccgcttccg cctccacctc cagtagttgt aggggtgcgc
     2281 tttagcgcat cccaccgagc aagtccgcga gcgcgtctct ttcttctccg tgtcat
//
LOCUS       DASTPM010000215         3962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_497531, whole genome shotgun sequence.
ACCESSION   DASTPM010000215 DASTPM010000000
VERSION     DASTPM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497531"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2574)
                     /locus_tag="VFR42_03790"
     CDS             complement(<1..2574)
                     /locus_tag="VFR42_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368305.1"
                     /translation="MPRLQTSEDVRLEDNRTRKEYWKRWGPYLSERQWGTVREDYSAN
                     GTAWEFFPHDHARSRAYRWGEDGIGGISDRHQIICFALALWNGRDPILKERIFGLTGN
                     EGNHGEDPKEYYFYLDSTPTHSYMRMLYKYPQTEFPYQQLVEQNRRRGRSQPEFELYD
                     TGVFAENRYFDVFAEYAKGSDEDIFIRITAWNRGPEKAPLHLLPTLWFRNTWSWGKDL
                     RRPTIRRSTPLDGILTAELQHWQYGKRWLLAHGQPEFLFTENETNNARLFATRNRSPY
                     VKDAFNEYLVHQNKAAVNPDQNGTKIAAYYRFDLPSGQCCVLKLRLTDIEPLGGMDRD
                     VEQVGTITSPGHQERAQEVPGTNDFGAGFDRLFEIRKTEADEFYASRIPEHLSDDAKN
                     VMRQAYAGMLWSKQFYHYDVKAWLEGDPACPTPPKERWHGRNKDWTHLYNDDIVSMPD
                     KWEYPWYAAWDLAFHAIPLAIVDPDFAKEQLILFLREWYMHPNGQLPAYEWAFGDVNP
                     PVHAWAAWRVYKIERRIRGVADRGFLEKVFHKLLLNFTWWVNRKDPDGANIFQGGFLG
                     LDNIGVFDRSAPLPTGGHLEQSDGTSWMGMYCLNMLAMALELAKDDPAYEDVASKFFE
                     HFVYIARAMNDFGSGGVSLWDEEDGFYYDVLHLPNGEEHFLKIRSMVGLIPLFAVETL
                     EPEIIERLPGFKRRMQWFIDNHPDVPEHIEMTQRSARGVRRLLSLVNRKQLKRVLARM
                     LDETEFLSPYGIRALSRYHLDHPYEVYVNGTTNRVDYEPAESTTGLFGGNSNWRGPIW
                     MPVNYLLIESLQKFHHYYGEDFKVPCPSRSKGDADLWQVACELSRRLCHIFVRGKDGR
                     RP"
     gene            complement(2791..>3962)
                     /locus_tag="VFR42_03795"
     CDS             complement(2791..>3962)
                     /locus_tag="VFR42_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368306.1"
                     /translation="GKKDRLSRISYKDFLLNIAKVDPGVVAFYQTRTNGEWGVGIDAE
                     PALDCWALGLPGFQGLKLAAGSAPRMSYSGAGYANGGSDRFHFPDGNASIARLLVRNL
                     IPTAVPGNSVEDVVTTSVNYARLDSPESSVRIRLNSTVVRVRNASRPHEDPHQAKEVT
                     VTYANGQEVFSVRAGACVLACWNMAIPYLCPDLPEKQKEALHYLVKVPLVYTSVGLRN
                     WTAFQKLGVSSIYCPGAYWNSVSLNATVNIGDYRSPRSPEEPILVHLSRTPCKPGLPA
                     REQHKAGRMELLVTTFETFERKIRDQLARTLSAGGFDPPRDIEAITVNRWPHGYSYEY
                     NPLFDTDWPEGQAPHLVGRERLGRIAIANSDSGATAYTDVAMDQAYRAVGELLAS"
BASE COUNT          877 a         1196 c         1049 g          840 t
ORIGIN      
        1 aggcctccgg ccgtctttgc cacgaacgaa aatgtggcac aaccgtcgtg aaagttcgca
       61 tgccacctgc cacagatccg cgtcgccctt cgaacgcgac gggcacggca ctttaaaatc
      121 ttctccgtaa tagtgatgaa acttctgcag cgattcgatc agcagataat tgaccggcat
      181 ccagatgggc ccgcgccaat tggagttgcc gccaaaaagc cccgtggtcg attcagcggg
      241 ctcatagtcc acgcgattcg tggtgccgtt cacgtacacc tcgtagggat gatccaggtg
      301 atagcgggac agtgcgcgaa ttccgtaggg cgaaagaaac tccgtctcat ccagcatgcg
      361 cgccaatact cgcttaagtt gcttccgatt caccagggag agcagccgtc gcacgccacg
      421 cgccgatcgc tgcgtcattt cgatgtgctc cggaacatcc ggatgattgt cgatgaacca
      481 ctgcatgcgc cgcttgaaac ccggcagtcg ttcgataatc tccggctcca gcgtttccac
      541 cgcgaagagc gggatcagcc cgaccatcga acgaattttc aaaaaatgtt cttcgccgtt
      601 cggcaaatgc agcacgtcgt aataaaagcc atcctcttca tcccacaacg acacgccgcc
      661 ggaaccgaaa tcgttcatcg cccgcgcgat gtaaacgaag tgctcaaaaa acttgctggc
      721 cacgtcctcg tacgccggat catccttggc cagctccagc gccatcgcca gcatattcag
      781 gcaatacatg cccatccaac tcgtcccatc cgattgttcc agatgtccgc cggtaggtag
      841 cggcgccgac cgatcgaaaa ctccgatgtt gtccaggccc aggaatccgc cctgaaaaat
      901 gtttgcccca tccgggtcct tgcggtttac ccaccaggta aaattcagca gcagcttgtg
      961 aaaaaccttc tccagaaatc cgcggtcggc tactccacgg atgcggcgtt caattttgta
     1021 aacacgccaa gctgcccacg catgcaccgg cggattcaca tcgccgaacg cccattcata
     1081 agcgggaagc tggccgttgg gatgcatgta ccactcgcgc aaaaatagaa tcagctgctc
     1141 tttcgcaaaa tccggatcca caatcgccag gggaatcgcg tgaaaggcca ggtcccacgc
     1201 ggcataccac ggatattccc acttgtccgg catggacaca atgtcgtcgt tataaagatg
     1261 cgtccaatcc ttgttgcgcc catgccaccg ttccttcggc ggcgtcggac aggccggatc
     1321 gccttccagc catgccttca cgtcgtagtg gtagaactgc ttggaccaca gcattcctgc
     1381 atacgcctgc cgcattacat ttttcgcgtc gtccgaaaga tgctccggaa ttcgcgaagc
     1441 gtaaaattca tccgcttccg tcttgcgaat ttcaaaaagc cgatcgaaac ctgcgccaaa
     1501 atcattggta ccgggaactt cctgcgcccg ctcctgatgt ccgggcgaag tgatagtgcc
     1561 aacctgctcg acgtctcgat ccatcccgcc aagtggctcg atatcggtga gccgcaattt
     1621 caaaacgcag cattgcccgg acggaagatc aaaacgatag tatgcggcta tcttggtgcc
     1681 gttctgatca ggattcaccg cggccttgtt ctggtggacc agatattcgt tgaacgcatc
     1741 ctttacatag ggcgaacggt tccgcgtcgc aaaaagccgc gcgttgttcg tttcgttctc
     1801 cgtaaaaaga aactccggct ggccgtgcgc aagcagccag cgtttgccat attgccagtg
     1861 ctgcaattcc gctgtgagta tgccgtcgag cggcgtggag cggcgaatcg tagggcggcg
     1921 cagatccttg ccccacgacc aggtattgcg gaaccacaac gtcggcaaca gatgcagcgg
     1981 tgccttttcc ggtccccggt tccacgctgt aatccggatg aaaatgtctt catcactgcc
     2041 cttcgcgtac tccgcgaata catcgaagta gcggttctcc gcaaatacgc cggtatcgta
     2101 cagttcaaat tccggctggc tccggccgcg acgccggttt tgttccacca attgctggta
     2161 agggaattcc gtctgcgggt acttgtacag catgcgcata tagctgtgcg tcggcgtcga
     2221 atccaaataa aaataatatt ccttgggatc ttcgccgtga ttgccttcat tgccggtcag
     2281 gccgaaaatg cgctctttca aaatcggatc gcgcccattc caaagcgcca gcgcaaaaca
     2341 aatgatttgg tgccggtcgc ttattccgcc gataccgtct tcgccccagc ggtacgcccg
     2401 cgaacgcgcg tggtcgtgcg gaaaaaattc ccacgccgtt ccgttcgcgc tgtagtcttc
     2461 acgcaccgtg ccccattgcc gctcggaaag ataggggccc cagcgcttcc aatattcctt
     2521 ccgcgtgcga ttgtcttcga gccgaacatc ttcactggtc tggagtctcg gcatgggggc
     2581 tgcggcctcg ttccggaaat gagtcgtcct atcgtcgcac caaaccaagc cgagtgggcc
     2641 gcgggacgcg aaacaccaac cacccgcgcc caaaaaccag aggcactggc gaaggacatc
     2701 ctggactcgg ataggatgcg ccagtcgcga taaagtgaca aacagttcgt gattcgaaca
     2761 ctcagtccat ccgaaagagt cgaatgctgt tcagctagcg agcaattcac cgaccgcgcg
     2821 atacgcctga tccatcgcca cgtccgtgta cgccgtggcg ccggagtcgc tgttggcaat
     2881 cgcaattctt ccaagccgct cgcggccgac aaggtgtggt gcctggccct cgggccaatc
     2941 cgtatcgaag agcggattgt attcatagct atagccgtgc ggccagcgat tgacggtaat
     3001 ggcctcgatg tcgcgcgggg gatcgaatcc gcccgcggaa agagtgcgcg cgagttggtc
     3061 gcgaattttg cgctcgaacg tttcaaatgt agtgaccaga agttccatcc ggccggcttt
     3121 gtgctgctcg cgggcaggca gcccgggctt gcagggcgtc cggctgaggt gcaccaggat
     3181 tggctcttct ggagaacgcg ggctgcgata gtcgcctata ttgaccgtgg cattcaggct
     3241 caccgaattc cagtaagctc ccgggcagta aatgctggaa acgccgagtt tctgaaaagc
     3301 cgtccagttg cgcaaaccga cgctcgtata caccagagga actttcacca aataatgcag
     3361 cgcctctttt tgtttttccg gcagatccgg acacagataa ggaatggcca tgttccagca
     3421 ggccagaacg cacgcaccgg cgcggacaga aaagacttct tggccatttg cataggtcac
     3481 tgtgacttcc ttggcttgat gcggatcctc atgcggcctg ctcgcgttgc gaacgcgtac
     3541 tacggtgctg ttcagtcgaa tccgaacgga agattcgggg gaatccaacc gagcgtagtt
     3601 caccgacgtg gtgactacgt cttcaacgga gttcccgggt actgccgtag gaatcagatt
     3661 gcgtaccagc aagcgcgcga tcgacgcatt gccatccgga aaatgaaaac ggtccgaacc
     3721 gccatttgca tatcctgcgc ctgagtaact catgcgtggt gcggagccag cggcgagctt
     3781 gaggccctga aatccgggaa gtccgagcgc ccagcaatcc agcgcgggct ctgcatcaat
     3841 tcctactccc cattcgccgt tggttcgcgt ttgataaaac gccaccacgc cgggatccac
     3901 cttcgcaatg ttcaacagga aatccttgta gctgatacgc gaaagccgat ccttctttcc
     3961 ag
//
LOCUS       DASTPM010000216         6337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_641712, whole genome shotgun sequence.
ACCESSION   DASTPM010000216 DASTPM010000000
VERSION     DASTPM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641712"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFR42_03800"
     CDS             complement(<1..776)
                     /locus_tag="VFR42_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368307.1"
                     /translation="MQGTLRIAYDALVRVLIAEDDPGVQRFVTKGLQEQAYAVDAVAT
                     GPEALELAEINSYDLIILDVMLPGMSGFEVCRQLRELGLKIPLLMLTARDAVEDRIAG
                     LDHGADDYLTKPFEFRELLARMRALLRRPAELKAAQLHVGDLTLDTAAQSATRAGRNI
                     PLTHKEYALLEYLVRNVNRVVGRAEIAEHVWNEQFDPFSNLIEVYVNRLRRKIDGAKE
                     APLLHTRRGAGYLLGLVETRGEASEAAGSGAAATREKNKP"
     gene            complement(801..1520)
                     /locus_tag="VFR42_03805"
     CDS             complement(801..1520)
                     /locus_tag="VFR42_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368308.1"
                     /translation="MSSAKILIVDDAQQVRRVLRTALSAEGYTIFEAGTGEEALESVR
                     QSTPDAILLDVNMPGIGGLETCREIRRTMDIPILMLTVRNAERDKVMALDAGADDYVV
                     KPFSMAELLARIRAALRRRLPQEKVTSFSTKDFAVDFETRQVTVQGRDVHLTPKEFEL
                     LRHLISNAGKPITHRRLLQAVWGPDYGDEPEYLRVMINQLRKKIESDPGRPKLILTEP
                     WVGYRFQAPREPRAPKEAKQD"
     gene            complement(1517..2980)
                     /locus_tag="VFR42_03810"
     CDS             complement(1517..2980)
                     /locus_tag="VFR42_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEU0368309.1"
                     /translation="MIPVIQRRTLRSFLALLLVAGITVFFRKALPHVNQTTVALSFLL
                     AILAVSAVWGMTVSVLMSIAAMLAFNYFFLPPVGTLTITDPQNWVALLAFLLTSITGS
                     RLSSRIRREADVANRRRREIEHLYSFSQKLLGEGNVIQLMNAIPNHIVESFESGAASL
                     YLADKQKFYRSGFGTLQLEEEQLRTAYEREEPYADAARGFCYGPVRIGMKSIGSFGIS
                     GAPLSRQTLEALGTLLGIAIERARAVEQLSRTEADRQGERLKSALLDSITHNFRTPLT
                     SIKASVTSLLSDHAPEGAQQRELLEIMDEECDRLNKLVEDASEMSKLEAGEIELQLRP
                     VTARELVETALANCKTSLGAREVRIQLPPYLPMVRADLPRAKEVLVHLLENANLYSEK
                     DQPIVISAESSGGLVTFAVADQGPGIDVMEQGLIFDKFYRGRDQRTVIQGTGMGLPIS
                     KAIVEAHGGTIAVTSQLNHGSVFTFTLPASRDGVERP"
     gene            3031..4155
                     /locus_tag="VFR42_03815"
     CDS             3031..4155
                     /locus_tag="VFR42_03815"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0368310.1"
                     /translation="MSKNPEEWLAVASPPEKARGNFKLFLGYAPGVGKTFAMLSEAIR
                     RHSRGEDVVIGVVETHGRKGIEELVTQLEAVPRKKIEYKGTLFEEMDVDAILARKPAV
                     VLVDELAHTNIPGSKNRKRYEDVQAVLAAKIDVISTLNIQHIESISPVVRNITGITIR
                     ETVPDWVPMTASETVMVDLTPEALQNRMRRGDVYLPAKVEQALNNFFRRGNLIALREL
                     ALRQVAEQVDRSLENYMAAKDIQKNWAVRERIAVCVSSNPAAQYLIARAARMARRLDS
                     ELYVIHVDTGRDNSDPSKKTLAANLRFAENLGAKVVQLKGLSVADSVAKVVREKHITH
                     VIFGRSVEHGWKKYLYLSAAHRFLRESPEVDVHIVTQEKD"
     gene            complement(4269..5207)
                     /gene="rbsK"
                     /locus_tag="VFR42_03820"
     CDS             complement(4269..5207)
                     /gene="rbsK"
                     /locus_tag="VFR42_03820"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEU0368311.1"
                     /translation="MNRKPIVVVGSINMDLVARAERIPLPGETVHGSALQMHPGGKGA
                     NQAVAAARLGYPVRMISSLGSDTFGEKLRAQLVQSGVDTSRVRTCEGPSGVALIAVAL
                     NGENAIVVTPGANALVSPSDIDENTDAIREAGVVLAQLEIPFETVQHLGKLCTRLNVP
                     LILDPAPARALTAELLEQVSWLTPNETEITQCIPEAKEVSRSQDAFEIVEALAKRVRR
                     GVVLKMGSRGAYVATNDGDRQQIDAFRVTAIDTTGAGDAFNGAFAAGLIAGKSPLESA
                     QFAVAASAVSVMRAGAQPSMPTMVEVNLLLDQSGRD"
     gene            complement(5228..6193)
                     /locus_tag="VFR42_03825"
     CDS             complement(5228..6193)
                     /locus_tag="VFR42_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU0368312.1"
                     /translation="MTRTLLIDTDGGSDDVVALIMALRSPAVRVAAITVIAGNVPVEQ
                     ATRNVLYTVELRGAQTPVYAGAAKPLLRDLVTAEWFHGKDGLGDHGYAPASREQESAH
                     AVDAIIETVLANPGMEIVTLGPLTNLALAIGREPRISKAIGRCIVMGGAPCCEGNVTP
                     AAEFNIWVDPEAAKMVFRAGLPIEVVGWHLCRGMAALNEKDIEKIRALKTPVAEFALR
                     CNSVAAEAYRVQTGEAGISLPDPIAMAIALQPGLCTSSSMHYVDVETASGLARGMTVV
                     DRLNVAGDERNKGQWEWAIKAQVKARVCWTLNIDGWKAALRAALA"
BASE COUNT         1264 a         2121 c         1658 g         1294 t
ORIGIN      
        1 tgcggcttgt tcttctcgcg cgtcgctgcc gctccagatc cagcagcttc cgacgcttcg
       61 ccacgagttt cgaccagtcc aagaagatac ccggccccgc gccgcgtatg cagaagtggc
      121 gcttccttgg cgccgtcgat cttgcgccgc aaacgattca cgtaaacttc gataagattg
      181 gaaaaggggt cgaactgctc gttccaaacg tgttcggcaa tttcagcacg gcccaccact
      241 cgattcacgt tccgaaccaa atactccagc agcgcgtact ctttgtgtgt gagggggatg
      301 ttgcgccccg cccgcgttgc tgattgcgcg gccgtatcca gcgtcaaatc gccaacatgc
      361 agttgcgccg ccttcaactc tgccgggcgc cgcaacagcg cgcgcatccg cgccagcagt
      421 tcgcgaaact caaacggttt ggtgaggtaa tcatccgcgc cgtgatcgag ccccgcgata
      481 cgatcttcca ccgcgtcgcg tgcggtcagc atcagcagcg gaattttcag tccaagctcg
      541 cgcagctgcc ggcacacctc aaagccgctc atgcccggca gcatcacatc cagaatgatc
      601 aggtcgtagg aattaatctc cgccagttcc agcgcttccg gccccgttgc gactgcgtcc
      661 accgcgtagg cctgctcctg cagtcctttc gttacgaagc gctggacgcc cggatcgtct
      721 tctgcaatca gtacccgcac cagcgcatca tacgcgattc gcagcgtccc ctgcacccgg
      781 ctcgaacacg aatgtgccgc tcagtcttgc ttcgcttctt tcggcgcccg aggttcacgc
      841 ggcgcctgaa accggtagcc cacccacggc tccgtcagga ttaatttggg ccgccccgga
      901 tcgctctcaa ttttcttccg cagctgattg atcattaccc gcaaatattc cggctcgtct
      961 ccgtaatccg gtccccacac cgcttgcagc agccgccggt gcgtgatcgg cttgcccgcg
     1021 ttcgaaatca agtgtcgcag cagttcgaac tctttgggtg tcaggtgaac atcgcgcccc
     1081 tggaccgtta cttgccgcgt ttcgaagtcc accgcaaaat cctttgtgga aaatgacgta
     1141 actttttcct gcggcagtcg gcggcgcagc gccgcgcgaa tccgcgccag caactctgcc
     1201 atgctgaacg gtttcactac gtaatcatca gccccggcat cgagcgccat cactttgtcg
     1261 cgctccgcgt tgcgtaccgt cagcatcaga atcggaatgt ccatcgtgcg gcgaatctcg
     1321 cggcaagttt ccagcccgcc gatacccggc atgttgacgt ccagcaaaat cgcgtccggc
     1381 gtggattgcc gaaccgactc cagcgcttcc tcgcctgtgc ctgcctcaaa aatcgtgtaa
     1441 ccttccgccg acaacgcggt ccgcagcacg cgccgcacct gctgcgcgtc gtccacgatc
     1501 aggattttcg cggaactcac gggcgctcca ctccgtcccg cgacgcaggc aacgtgaacg
     1561 taaataccga accgtgattc agttggctgg tcacggcgat cgttccgccg tgcgcttcca
     1621 caatagcctt tgaaatcggc aagcccattc cggtgccctg aatcaccgtg cgctggtctc
     1681 ggccccgata aaatttgtca aaaatcaatc cctgctccat cacgtcgatg cccggtccct
     1741 gatccgccac ggcgaacgtc accaacccgc cgctgctttc cgcgctgatc acaatcggct
     1801 ggtctttttc cgaatacaga ttcgcatttt ccagcaggtg taccaatact tccttcgccc
     1861 gcggcaggtc cgcgcgcacc atgggcaggt atggcggcaa ttgaattcgc acttcacgcg
     1921 cgcccagcga agttttgcag ttcgccagag cggtttcgac caattcgcgc gccgtcaccg
     1981 gacgcagctg cagctcgatt tccccggctt ccagcttcga catttctgac gcgtcctcga
     2041 ccagtttgtt caagcggtcg cactcttcgt ccatgatctc cagtagttcg cgttgttgcg
     2101 ctccctccgg cgcatggtcg gacaacaagg aggtaaccga cgctttgatt gaagtcagcg
     2161 gcgtgcggaa gttgtgcgta attgaatcga gcaacgccga tttcagccgc tcgccctgac
     2221 gatccgcttc cgttcggctg agctgctcca cggcccgcgc tcgctcaatc gcaattccca
     2281 gcagtgtccc cagcgcctcc agcgtctgcc gcgaaagtgg cgcgccggaa attccaaagc
     2341 ttcctatgga tttcatgccg atccgcaccg gcccgtagca aaaacctcgc gccgcgtccg
     2401 cgtacggttc ttcgcgctcg taggccgtcc gcagttgttc ttcctccagc tgcagcgtcc
     2461 cgaagcccga ccggtaaaat ttctgcttgt cagcgagata caggctggct gcgccgcttt
     2521 cgaaagactc cacaatgtga ttcggaatcg cgttcatcag ttgaatcacg ttcccttcgc
     2581 ctagcagttt ctggctgaag gagtagagat gttcgatttc gcgccgccgc cggttggcca
     2641 catctgcctc gcgccggatt cggctggaca gccggctgcc cgtgatcgac gtcagcaaaa
     2701 acgccagcaa ggccacccag ttctgcgggt cggtgatagt cagtgtccct acgggcggca
     2761 gaaagaaata gttgaacgcc agcatcgccg cgatcgacat cagcaccgac acggtcatgc
     2821 cccaaaccgc ggaaactgct aaaattgcta aaagaaacga caatgccacc gtggtctggt
     2881 ttacgtgggg caacgccttg cggaaaaaca cggtgattcc tgcaaccagc agtagggcta
     2941 aaaagcttcg caacgttcgc cgctggatga ccggaatcat gccgcaattc tagcgcagtc
     3001 gctgccatcc acggattagg gaagcataaa gtgagcaaaa atcctgaaga atggctggcg
     3061 gtcgcctcgc ctccggaaaa ggcgcgcggc aacttcaaac tgtttttggg ttacgctccc
     3121 ggcgtcggta aaaccttcgc catgctgagc gaagccatcc gccgccacag ccgcggcgaa
     3181 gacgtcgtca tcggtgtggt ggaaacgcac ggccgcaaag gtatcgaaga gctggtcacc
     3241 cagctggagg cagtcccgcg caagaaaatc gaatacaaag gcacgctgtt cgaagagatg
     3301 gacgtggatg ccatactcgc ccgtaagccc gccgtggtct tggtggatga gctggcccac
     3361 accaacattc ccggcagcaa gaatcgcaaa cgctacgaag acgtgcaggc cgtcctggcc
     3421 gcgaaaattg atgtcatctc cactctcaac atccagcaca tcgaaagcat ttcgcccgta
     3481 gttcgcaaca tcaccggcat caccatccgc gaaaccgttc cggactgggt gcccatgacc
     3541 gccagcgaaa ccgtcatggt cgaccttact cccgaggccc tgcaaaaccg tatgcgccgc
     3601 ggcgacgtct acctgccggc caaagtcgaa caggctctaa acaacttttt ccgccgcggc
     3661 aatctcattg ctctccgcga gctcgctttg cgccaggtgg ccgaacaggt cgatcgcagc
     3721 ctggaaaatt acatggccgc caaggacatc cagaaaaact gggccgtccg cgaacgcatc
     3781 gccgtctgtg taagctccaa tcccgccgcg caatatctga tcgctcgcgc cgcccgcatg
     3841 gctcgccgcc tggactcgga gctctacgtg attcacgtcg acaccggccg cgacaattcc
     3901 gatccaagca agaaaactct cgccgccaac ctccgcttcg ccgaaaatct cggcgccaag
     3961 gtcgtccagc tcaagggctt gagcgtggct gactccgtgg cgaaagtggt ccgcgaaaag
     4021 cacatcaccc acgtcatctt tggccgctca gtcgaacacg gctggaaaaa atatctctat
     4081 ctttccgcag cccatcgctt tttgcgcgaa tcgccggaag tcgacgtcca catcgtcact
     4141 caggaaaagg actaacggtt gggcggaaag ctttcttctc ttacggaaaa cgggcaaccg
     4201 aaaacacata tcgtgcagcg gcagccttgt agcggccttt tttccgcgga ctcatgcgtg
     4261 ggaaacgcct agtcgcgacc actctgatct aacagtaggt tcacttctac catcgtcggc
     4321 atcgacggct gcgctccggc tcgcatcaca gaaaccgcgc tggcagccac cgcgaactgg
     4381 gcgctctcca gcggcgattt ccccgctatc agtcccgctg caaaagctcc gttgaacgcg
     4441 tcgcctgccc cggtagtgtc gatcgcagtc acacgaaatg cgtcaatctg ctggcggtcg
     4501 ccgtcgtttg tagccacata cgcgccgcgc gagcccatct tcagcactac tccgcgccta
     4561 actcgctttg ccagcgcttc cacaatctcg aaagcgtcct gcgaacgcga aacttccttc
     4621 gcctccggaa tacactgcgt gatctccgtc tcgttcggcg tgagccagct gacttgctcc
     4681 agcaattccg ccgttaatgc cctcgccgga gccggatcca gaatgagtgg aacattcagt
     4741 ctggtgcaaa gttttcccag atgctgcacc gtctcgaacg gaatctccag ctgcgcgagc
     4801 accacacctg cttcgcggat cgcgtccgtg ttctcatcaa tgtcgctcgg cgacaccaga
     4861 gcattcgcgc ccggcgttac cacaatcgca ttctcgccat tgagagctac cgcaatcaac
     4921 gcgactcccg atggaccttc gcacgtgcgc acgcgggaag tgtcgacccc actctgcacc
     4981 agctgcgcgc gcaatttctc gccgaacgta tcggaaccca ggctgctgat catgcgcacg
     5041 gggtacccaa gccgcgcagc agcgactgcc tggttcgcgc cctttccgcc cggatgcatc
     5101 tgcaaggcgc tgccgtgaac ggtttctccc ggaagtggaa tccgttcggc acgcgcaacc
     5161 aagtccatgt tgatgcttcc gaccaccacg atcggttttc gattcatcgc caccgctcat
     5221 cacctggcta cgcaagcgcg gcgcgcagcg cggccttcca accgtcaatg ttgagtgtcc
     5281 agcaaacgcg ggcctttacc tgcgccttga tcgcccactc ccactggcct ttattccgct
     5341 cgtcacctgc cacattcaga cggtccacca ccgtcatccc ccgcgccaga ccgctggccg
     5401 tttccacgtc gacgtaatgc atgctcgacg atgtgcaaag tcccggctgc agggcgatcg
     5461 ccattgcgat cggatccggc agcgaaatac ccgcttctcc ggtttgcacg cgatacgcct
     5521 ccgccgcaac gctattgcag cggagggcaa attccgccac cggcgttttc aaagcacgga
     5581 tcttctcgat gtctttctcg ttgagcgccg ccatgccgcg gcaaagatgc cagccaacca
     5641 cctcgatggg cagtccggca cggaaaacca tttttgccgc ttccggatct acccagatgt
     5701 tgaactcagc cgccggcgtg acgttcccct cgcagcaggg cgcgcctccc atcacaatgc
     5761 aacgtccgat cgctttgctg atccgcggct ctctgccgat cgccagcgcc agattcgtca
     5821 gtggacccag agtaacaatt tccattccag gattggccag taccgtttcg atgatcgcgt
     5881 ccaccgcgtg cgcggattcc tgctctcgcg acgcgggcgc gtatccgtgg tctcccaggc
     5941 cgtccttccc atggaaccac tctgccgtca ctagatctct cagcagaggt ttcgcggcgc
     6001 ctgcgtaaac cggagtctgc gctccgcgca gttccacggt gtacagcaca ttgcgtgtgg
     6061 cctgttcgac cggtacgttt ccggcaatca ccgtaattgc tgctacacga actgcgggcg
     6121 accgcagcgc catgatcagc gccacgacat cgtctgaacc gccatccgta tcgattaaca
     6181 aagttcgcgt catagccgac tctgctcgtc ggcttcgcga tggcgctcga ggcggtcgat
     6241 gaattgttca aacgaaggag caatctcaac gatgatggcg ttttcgccgg ggtagaaaat
     6301 cttgaccggc gctggtgtcg tgctaatgtc tagaaaa
//
LOCUS       DASTPM010000217         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1131664, whole genome shotgun sequence.
ACCESSION   DASTPM010000217 DASTPM010000000
VERSION     DASTPM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131664"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="VFR42_03830"
     CDS             complement(<1..2040)
                     /locus_tag="VFR42_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0368313.1"
                     /translation="MEPERWQRIEQLYYAALELGEGARASFLDNSCSGDDGLRQEVES
                     LLIQERNAEGFLEVAVLESLVQARAPREEPQAKGQDDDPLEISGRSVSHYRVIEKLGG
                     GGMGIVYKAEDSRLGRFVALKFLPQVANADRVAIERFRREARAASALNHPHICTIHDI
                     GEHEGRQFIVMELLEGQTLKRAMAGGAVEPARVIKIGLQIAEALQAAHTKGIVHRDIK
                     PANVFVTERWQVKVLDFGLAKLLAPVNSEATLLEEEICTRGPVGTLPYMAPEQALGRN
                     IDARTDIYGLGMVLYEMTAGRRPFREDFPTHLIDDILHKAPPSLGSFGGGTPEGLNQI
                     VLRCLEKNPAARFSSAGELIVALEGILSGPGSPVANARRPSRRTMLAAYGLVALLLAG
                     ASLVLNVGGWRARLLTPSPSINSLAVLPLANLSGDSSQDYFSEGITEALTTELAQVSA
                     LRVISRTSAGHFRNSRETVPEIARKLNVDAIVEGSVTRSANRVRITAHMIDIQDDRDV
                     WARTYEKDISDILALQDEVARDIAAEIRIKITPQEKVRLGTDRQTNPKAYDAYLRGRY
                     LWSQRNAEAITKAVSYFQEAVREDPNFAAAYSGLADCYWVGWGARVDIALADSYAQKA
                     VELQPDLAEAHASLGIVRHFERNMPAAEREFKKALDLNPNYSMAHHFYSAYLLGQG"
     gene            complement(2030..>2542)
                     /locus_tag="VFR42_03835"
     CDS             complement(2030..>2542)
                     /locus_tag="VFR42_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0368314.1"
                     /translation="DHLMPIVYEELHHAARRYMRRENSGNTLQTTALINEVYLRLVNV
                     PGVSWQNRAHFFAVCAKLMRRILVDRARARRAQKRGGGMAAPAFDEAFFVPNAPSSDM
                     VALDEAMNRLSKIDERKSLVVEMRFFGGLSIEESAEVLNVSAETVKRDWKLAKVWLLR
                     ELSPENHDGA"
BASE COUNT          499 a          770 c          664 g          609 t
ORIGIN      
        1 gccttggcct agcaagtagg ccgagtaaaa atgatgggcc attgaatagt tgggatttag
       61 gtctaaggcc tttttaaatt cgcgttcggc ggcaggcata ttgcgctcaa agtgacggac
      121 gattcccagg gaagcgtggg cttctgcgag atccggctga agctcgactg ccttttgggc
      181 atacgagtcc gcaagtgcaa tgtccactct tgcgccccag ccaacccagt agcaatcggc
      241 cagtccggaa taagccgccg caaaattggg atcctcccgt accgcttctt ggaaataact
      301 caccgctttc gtaatcgcct ctgcgttcct ttggctccaa aggtagcgac cgcggaggta
      361 cgcatcgtaa gctttaggat tggtttgcct gtccgtcccc aggcgaacct tttcttgcgg
      421 tgtgattttt atgcgaatct ctgctgcgat atcgcgggcc acttcgtcct gcaacgctag
      481 gatgtcgctt atgtctttct cataggttct ggcccacacg tcgcgatcat cctgtatgtc
      541 aatcatgtga gccgtaatcc gtacgcggtt cgcggaacgc gtaaccgagc cttccacaat
      601 cgcatcgacg ttgagtttcc gagcaatttc gggcactgtc tcccggctat ttctaaaatg
      661 tcccgcggac gtgcgcgaaa tcacgcgaag cgcgctgacc tgcgcgagtt cggtcgttaa
      721 cgcctcggtg attccttcac tgaaataatc ctggctagaa tcgccggaaa gatttgcgag
      781 cggcagaact gcaagcgaat tgatgctcgg ggaaggtgtc agcagccgcg cgcgccatcc
      841 gccaacattc agcacgaggc tggcgccggc tagcagcagc gccacaagtc cgtatgcagc
      901 caacatcgtc cgtcgagaag ggcgccttgc gttagccact ggactgccgg ggccactcaa
      961 aatcccttcc agtgcgacaa tcaattcacc tgcagacgaa aaccgcgctg ccggattctt
     1021 ctcgaggcac cgcagcacaa tttgattcag cccttcgggc gtgccgccgc caaagcttcc
     1081 tagtgatggt ggggccttgt gcagaatgtc gtcgatcagg tgcgtaggga aatcctcacg
     1141 aaaggggcgc ctgccagcgg tcatttcgta aagcaccatt cccagcccgt aaatgtccgt
     1201 acgcgcatca atgtttcgcc cgagtgcttg ttccggagcc atgtagggca acgttcccac
     1261 cggacctctc gtgcatatct cttcttcgag aagtgtagct tccgaattca ccggagcaag
     1321 cagtttggcc agcccaaagt ccagcacctt cacctgccat cgctccgtca caaaaacgtt
     1381 tgccggtttt atgtctcgat gcacgattcc tttcgtatgg gccgcttgta gtgcctcggc
     1441 gatttgcagc ccgatcttga taactctcgc gggctccacc gccccgccgg ccatggcgcg
     1501 tttcaacgtc tgtccttcga gcagttccat cacgatgaac tgccgccctt cgtgctcgcc
     1561 aatgtcgtgg atcgtacaga tgtgtggatg attcaacgcc gaagcggccc gcgcctcgcg
     1621 tcggaatcgt tcgatggcta ctcgatcggc attggcgact tgcggtagga acttgagtgc
     1681 cacgaacctt cccaggcggc tgtcttcggc cttgtatacg atccccatgc ctccgccgcc
     1741 aagtttttca attactcgat agtgcgaaac ggaccttccg gatatttcca gtggatcgtc
     1801 atcttggccc ttagcttgag gctcttcccg gggcgctctt gcttgcacca ggctttccag
     1861 gacagcgact tccaggaagc cctcggcgtt tctttcctgt atcaacagcg actcgacttc
     1921 ctgccgcagg ccgtcgtcac ctgagcacga attgtctaga aaactcgcgc gtgccccttc
     1981 ccccagctcc agcgctgcgt aatacaattg ctcgattcgt tgccagcgtt caggctccat
     2041 cgtgattttc cggactgagt tcgcgcaaaa gccaaacctt ggctagcttc caatctcgtt
     2101 ttaccgtctc cgcggaaacg ttcagtactt cggctgattc ttcgatgctt aggcccccga
     2161 agaatctcat ctccacgaca aggctctttc tctcgtcgat tttgctcaag cggttcattg
     2221 cctcatccag cgccaccatg tcagaagatg gtgcgtttgg cacgaagaac gcttcatcaa
     2281 atgccggagc ggccatgccg ccgccgcgct tttgtgcgcg cctggcccgt gcccgatcaa
     2341 ccaggattcg tctcatcagc ttcgcgcaaa cagcgaaaaa gtgcgccctg ttctgccagc
     2401 tgactcccgg aacgttcacc aggcgaaggt agacttcgtt gatcaacgcc gtggtttgca
     2461 gggtgttacc ggaattttcc cgtcgcatgt agcgccgtgc tgcgtgatgt aactcctcgt
     2521 aaactatggg catgagatgg tc
//
LOCUS       DASTPM010000218         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_526497, whole genome shotgun sequence.
ACCESSION   DASTPM010000218 DASTPM010000000
VERSION     DASTPM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526497"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..921)
                     /locus_tag="VFR42_03840"
     CDS             complement(289..921)
                     /locus_tag="VFR42_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU0368315.1"
                     /translation="MNGSQKQRQRGGIITTLVALLFLALLCLAIYLARHPLLRFAGEE
                     LIVEDTLEKSDAIILLSDDNFYADRATRAAELYRQNLAPVVVASGIRLRPYAGIAELM
                     THDLVERGVPKEHIVPFPHDADDTREEAAALNTLIAQKGWKSVIIVTSNYRTRRARLI
                     FRRTFPAGVRVEMASAHDGDFDPANWYQHGRAIRRFEHEVLGLVAAHWQI"
     gene            complement(918..1892)
                     /locus_tag="VFR42_03845"
     CDS             complement(918..1892)
                     /locus_tag="VFR42_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEU0368316.1"
                     /translation="MEIRRDPITQSWVVVGHRDSPENDSCPFERPAIDKQQTILSVPA
                     EGPWQVRVLPHPDPLYRIEGEPGRLPDGMYDRMGPTGAHEVVVESPDHNKRLSQLSDE
                     EIDRVLAVWVSRVADLKKDIRLKYVSVFKNQGELAGEEWSHAHSEITGTIFVPRRIKY
                     ELRAAREWFKEKERCVFCDIVRQEEKKGKRIVDVQGDYYALCPYAARVPYEVWLLHRR
                     HNHLFEQPKAGSNRKQLAALLGRVLRRLEKISPAFHLVVHTAPSTLSKKGELAGYWKT
                     LADDFHWHIEILPIVEKRSKSYSIKETYFNAVLPEQAAEQLRAIDPNS"
     gene            2094..2543
                     /gene="tadA"
                     /locus_tag="VFR42_03850"
     CDS             2094..2543
                     /gene="tadA"
                     /locus_tag="VFR42_03850"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEU0368317.1"
                     /translation="MITDADYMQLALEEARLAAEAGEVPIGAVLVRDEQVLARGGNRT
                     IRDCDPTAHAEIVILREASRAMQNYRLSGTTMYVTLEPCAMCAGAIIQARVPRVVYGA
                     DDPKGGAVRTCFAVLSHPKLNHQVQTTAGLLAEECAAVLHKFFTVRR"
     gene            2640..2729
                     /locus_tag="VFR42_03855"
     tRNA            2640..2729
                     /locus_tag="VFR42_03855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2674..2676,aa:Ser,seq:cga)
     gene            complement(2785..3933)
                     /locus_tag="VFR42_03860"
     CDS             complement(2785..3933)
                     /locus_tag="VFR42_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEU0368318.1"
                     /translation="MEERMRFVSRLKDGESMASLCREFGISRVTGYKVYERYKECGLE
                     GLTDRARTPYRYANKLPAQLEAMIVSMRREKPTWGARKLRDRLLRKLPNDVRVPACST
                     IHAILDRHGLVTRQRRSRTKTEGTPLSQGLAPNALWCTDYKGEFMLGDKRYCYPLTVS
                     DHASRYLLLCEAMESVKEQGAFTAFERLFKERGLPLAIRSDNGVPFASPNGLFNLSRL
                     AVWWLRLGITIERIQPGHPQQNGRHERMHRTLKIEATRPAGSNFLQQQAKFDAFVREF
                     NNERPHEALAMKYPADVYKASTRPYRGIGELSYPFHDRTALVTCCGRICIYKKKINLS
                     TSLAGQAVGIREVDDGIWLVSFMDYDLGYIDLEERTLQPLNNPFGPKV"
BASE COUNT          861 a         1167 c         1190 g          904 t
ORIGIN      
        1 aggagtactt gcctgcggaa ttgcggcact gggcggcata gcgggttccg cgacgccgga
       61 agactttgac tcttctttcc tccacattcg acacctcgac agggacagtt ggattttcga
      121 agtcgccagt cgtcgagcgg gagggcggcg cgacctcaga aacttcaaga tagtacagca
      181 cttactctat gtaaagcaga atgagacaaa aacagtatct cattggccac ttcgggtacc
      241 agtggttctg cgcggcacgg gccatgcctt gtggaaaccc gctaacggct aaatctgcca
      301 gtgtgccgcg acaagtccca ggacttcgtg ttcgaagcgt cttatggccc gaccgtgctg
      361 ataccagttg gccgggtcaa aatcaccatc gtgagcgctg gccatctcca ctcgcacgcc
      421 tgcgggaaac gtcctgcgga aaattagccg ggcacggcgt gtgcgatagt tggacgttac
      481 gataatgacg ctcttccagc ccttttgcgc gatcagcgta ttcagcgctg ccgcttcttc
      541 gcgcgtgtcg tcggcatcgt gcgggaacgg cacgatgtgt tctttgggca cgcctcgctc
      601 gaccaaatcg tgcgtcatca attcagcgat tccggcataa ggccgcaggc gaattccgct
      661 ggccaccacc acgggcgcca ggttctggcg gtatagctcc gccgcccgcg tggcccggtc
      721 cgcataaaaa ttgtcgtcgc ttaaaagaat gatggcgtcg gatttttcca aggtgtcttc
      781 gacgatcaat tcttcgccgg caaaacgcag cagcgggtgc cgcgccagat aaatcgccag
      841 gcacaacaac gccagaaaca gcagggcgac caaggtggtg atgattccgc cgcgctgccg
      901 ctgcttctgg cttccgttca tgagttggga tcgatggccc gcaactgttc ggcagcctgc
      961 tccggcagca ccgcgttaaa atacgtttcc ttgatgctgt aggacttgct gcgcttttcc
     1021 acgatgggga gaatttcgat gtgccagtgg aaatcgtcgg ccaacgtttt ccaatacccg
     1081 gccagttcgc cttttttgct caaagtgctg ggcgcggtgt gcaccaccag atgaaacgcc
     1141 ggcgagattt tctccaggcg ccgcaggact cggcccagca aagccgccaa ttgcttgcga
     1201 tttgaaccgg ccttcggttg ctcgaacaaa tgattgtggc gtcgatgcag caaccacact
     1261 tcatagggga cgcgggccgc gtacggacaa agcgcatagt aatcgccttg cacatctaca
     1321 atgcgcttcc ctttcttctc ttcctggcga acgatatcgc aaaacacgca gcgctccttt
     1381 tccttgaacc attcgcgcgc ggcgcgcagc tcgtacttga tgcggcgcgg cacgaaaatg
     1441 gttccggtga tttcggagtg cgcatgcgac cattcctcgc cggccaactc gccctggttc
     1501 ttgaacacgc tcacatactt aagacgaatg tcctttttca ggtccgcaac gcgggaaacc
     1561 cacaccgcga gtacgcgatc gatttcctcg tcgctcaatt gcgacaggcg cttgttgtgg
     1621 tccgggcttt ctaccactac ttcgtgcgct ccggttggtc ccatgcgatc gtacatcccg
     1681 tccggcaggc ggcccggctc gccttcaata cgatagagag ggtcgggatg cggcagcact
     1741 cgcacctgcc agggcccttc agcgggaacg gagagaatcg tctgctgctt gtcgatggcc
     1801 gggcgctcga aaggacaaga atcgttttcc ggcgaatcgc ggtggccgac aactacccag
     1861 ctttgtgtga tcggatctcg gcggatttcc attcgtatgt tgtccaggtc aagagcaaat
     1921 gctatcgcgc aaccgtgctt tatcctcgat ctttgcgtat tcagttgtgt cgtgagtcgc
     1981 gtttagtttc ggtgcggcgt tgcgcaaagt caagcgcgcg gagagtcagc gataaggctt
     2041 gatggcactc ccgccgcgcc atcttgctgc ataatacttg gaacacaaca ctcatgatta
     2101 ctgacgcaga ctacatgcag ttggcgctgg aggaagcgcg cctggcggcc gaagcgggcg
     2161 aagtgcccat cggtgctgta ctggtacggg acgagcaggt tctggcgcgc ggcggcaacc
     2221 gcactatccg cgattgcgac ccgacggcac acgccgaaat tgtgatcctg cgcgaagcgt
     2281 cgcgcgccat gcagaactat cgcctgagcg gaacgaccat gtacgttacg ctggagcctt
     2341 gcgccatgtg cgcgggcgca atcattcagg cgcgcgtgcc tcgggtggtg tatggcgccg
     2401 atgatccgaa aggcggcgcg gtgcgcacgt gctttgcggt gctatcgcat cccaagttga
     2461 atcatcaggt gcaaacgaca gcggggcttc ttgcagagga atgcgcagcg gtgctgcaca
     2521 aatttttcac cgtacgccgc tagttccctg ctcttgcgca cattcttccg tcgcaactcc
     2581 ctgctcggct gccgttgccg tcgcgcgaaa tggtctgcta atcttaagga accgaccgcg
     2641 gagaggtgac tgagcggttg aaagtactcg cctcgaaagc gagcgtacgg gaaaccgtac
     2701 cgtgggttcg aatcccaccc tctccgccat aactcaattg tacagtccgg agacatccct
     2761 tacagaacgt tcctgagaca tactttacac ctttgggcca aaagggttgt ttaaaggctg
     2821 cagagtcctt tcctcgagat cgatgtaacc gagatcgtag tccataaagc tcaccaacca
     2881 gataccgtcg tcgacctctc tgatacccac agcttgacct gcaagagaag tgctgagatt
     2941 gattttcttt ttgtaaatgc agattcttcc gcagcaggtg acaagagctg tccgatcgtg
     3001 gaaaggatag gagagttctc cgatgccacg ataaggccgc gtggaagctt tgtagacgtc
     3061 ggcggggtac ttcatagcga gagcttcatg cggacgctcg ttgttaaact cacgcacgaa
     3121 ggcatcgaac ttggcttgct gctgaaggaa attagaaccg gctggccgtg tagcttcgat
     3181 ctttaaggtt cgatgcatgc gttcatggcg accattctgt tgaggatggc caggctggat
     3241 acgctcgata gtgatgccga gccggagcca ccaaacggcc agcctgctta agttgaagag
     3301 accattgggt gaggcgaagg gaacgccgtt gtcggaacga atggctaggg gcaggccgcg
     3361 ttccttgaaa aggcgctcaa aggccgtaaa cgcgccctgt tctttgaccg actccatggc
     3421 ttcgcagagc aaaagataac ggctggcgtg gtcggaaacg gtgagcggat agcagtatct
     3481 tttatccccg agcatgaact cgcccttgta gtcggtgcac cacaaagcat tcggagcaag
     3541 cccttgggat agcggagtac cttccgtttt tgtgcgagag cgccgttgcc gggtcaccag
     3601 tccatggcgg tccaggatgg cgtggatggt gctgcaagcg ggtactcgaa catcattggg
     3661 caacttgcgt aacaagcgat ccctgagttt gcgagcgccc caagtgggct tctcgcggcg
     3721 catggagacg atcatggctt cgagctgcgc cgggagctta ttggcgtagc ggtagggggt
     3781 tcgcgcgcgg tcggttaatc cttccagccc acactctttg tagcgttcat agactttgta
     3841 tccggtaacc cgggagatgc cgaactcgcg gcacaaggag gccatgctct ccccatcctt
     3901 cagccgcgat acgaaccgca tgcgttcttc cacgacagac cgttccttcc atggcatgga
     3961 cacatccgtg ctggactagt ccagcacgga ggtgtaaacc atgtctccgg aacgttttgt
     4021 aaagggatga tccgaccgct catgatgatt caactaccct tacgcggctg agcgactcga
     4081 aaaccccata aaaaccagag attgtggacg gcaattggac gg
//
LOCUS       DASTPM010000219         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1189675, whole genome shotgun sequence.
ACCESSION   DASTPM010000219 DASTPM010000000
VERSION     DASTPM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189675"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VFR42_03865"
     CDS             <1..1130
                     /locus_tag="VFR42_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HEU0368319.1"
                     /translation="PTIDGSTWYPFSGHLLFTSESGSNASIMQATLDVPSKVEDISGI
                     VGRAAYEGVQADHWGRLILVEDSSGATGTAFPHARQPNSFVYRFLPNSPSDLKAGGKL
                     QVLQVKSKAHAGAIVFHAGSADADISSQDQKDLHTYGNVFQTTWVTIHDTAVNGFAPY
                     SANALAKTAGGTPFKRPENGQFRPGTNFSEFIFVATGDTNALTEAAAFGGFGAIFRLH
                     LSGDSGTLALVFRGDLTHTGMDNCGFWDQNRIVFVEDAGDGLHGQRNALDSAYLFDLN
                     TDYSNPTNKPVRVLAEGRDASATLDSQFSGMTGFQNDGDNEITGWHESDGDPGSSGLL
                     GAKIPTPFRGGWRVFFTQQHGDNNTFEILRKDKAGDDDDRD"
     gene            1264..2166
                     /locus_tag="VFR42_03870"
     CDS             1264..2166
                     /locus_tag="VFR42_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEU0368320.1"
                     /translation="MKTKALIRESADLAKPYRVFKGDNFRLKDFDPEDTSGMKDKKQA
                     KEILEHSRDLLSQFQEKLYAQDRWALLIIFQAMDAAGKDGAVTHVMSGINPQGCDVTS
                     FKAPSKEELDHEYLWRAHRAVPQRGKIGIFNRSYYEEVLVVRVHQDLLNAQQLPKELI
                     NKHIWEERYEDINNFEKYLGRNGIIVMKFFLHLSKSEQKKRFLERLEMPEKNWKFSMA
                     DVKERSYWKDYQQAYEEMIQNTATKHAPWHVIPADNKWFTRLAVAGAIIEKLHSLDLQ
                     FPEVDDDKKKELAQVRKALLAGKE"
     gene            2274..2747
                     /locus_tag="VFR42_03875"
     CDS             2274..2747
                     /locus_tag="VFR42_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0368321.1"
                     /translation="MNHVAAVIGAGFVGKAHIEALRRIPVRVKGTLVSSGDRSSAAAK
                     ALGLERAYSSVEEIAADPEITAVHICTPNYVHFGQSATLLRAGKHVMCEKPLAMDSRE
                     SAQLVALAKETNRVDGVAHNLRYYPLCIQAKALVDSGAIGKVRLVHGGFLQDWLT"
     gene            2769..3419
                     /locus_tag="VFR42_03880"
                     /pseudo
     CDS             2769..3419
                     /locus_tag="VFR42_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(3520..>3822)
                     /locus_tag="VFR42_03885"
     CDS             complement(3520..>3822)
                     /locus_tag="VFR42_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368322.1"
                     /translation="GSRQIARSLSRRFAKVLEFPVTEPSNLLGDFRPVAPLKTLDQLG
                     DQRSDLGSVERIRDEIVPADDETTLFAFLFEPFRRAYIEIERLGGFATPRTNIRAN"
BASE COUNT          887 a         1269 c          933 g          733 t
ORIGIN      
        1 tgccgaccat tgatggctcg acttggtatc cattttcggg ccatctgctg ttcacttccg
       61 agagcggctc caacgccagc atcatgcaag ctactctcga tgtgccttcc aaagtcgaag
      121 acatttccgg catcgtcggc cgcgcagcgt acgaaggcgt acaagccgat cactggggtc
      181 gcctcatttt ggttgaagac tccagcggcg ccacgggaac cgcgttcccg cacgctcgac
      241 agccaaacag ctttgtctat cgcttcctac cgaatagtcc ttccgacctg aaggcaggcg
      301 gcaagttgca ggttctccag gtgaaatcga aggcccacgc cggcgcaatc gtgttccatg
      361 cgggcagcgc cgacgctgac atctcctcgc aggatcaaaa ggatctacat acctacggca
      421 acgttttcca aaccacgtgg gtcaccattc acgacactgc ggtgaacggg tttgcgccat
      481 acagcgccaa tgccctggcg aaaaccgctg gcggcacccc cttcaagcgt ccggaaaacg
      541 gccagttccg gccgggcacc aacttctctg aatttatctt cgtcgccacc ggcgacacca
      601 acgccctcac cgaggccgct gcctttggcg ggtttggcgc aattttccgc ctccatctct
      661 cgggagactc cggcacgctc gcgcttgttt tccgcggcga cctgacccac accggcatgg
      721 acaactgcgg tttctgggac cagaaccgca tcgtctttgt cgaggacgcg ggtgacgggc
      781 ttcacggcca acgtaacgcc cttgattccg catatctctt cgacctgaac accgactaca
      841 gtaatccgac caataaacct gttcgcgtgc tcgccgaagg ccgcgatgcc agcgccacgc
      901 tggactcgca attcagcggt atgaccggct tccagaacga cggcgacaac gaaatcaccg
      961 gctggcatga atccgacggc gatcccggca gcagcggcct gctcggcgcc aaaatcccca
     1021 ctcccttccg cggcggctgg cgcgttttct tcacccagca acacggcgac aacaacacct
     1081 ttgaaattct cagaaaggac aaagccggcg acgacgacga tcgcgactaa attcgtagtc
     1141 aggtatttcg tctgatcgcg catccccgaa tgacgaatgg cccggctctt tcgagctggg
     1201 ccatttttat tccccgcgca agaatagccc gttctttttc cttcctgtac tagactgaag
     1261 cctatgaaaa ccaaagcact catccgcgaa tccgccgatc tggccaaacc ctatcgcgtc
     1321 ttcaagggag acaacttccg cctcaaggac ttcgaccccg aagacaccag cggaatgaag
     1381 gacaagaagc aggccaaaga gatactggaa catagccggg acctgctttc tcaattccag
     1441 gaaaaactct acgcgcagga tcgctgggcg cttctcatca tttttcaagc catggacgcc
     1501 gccggcaaag acggcgccgt cacgcatgtc atgtccggca tcaatccgca gggctgcgac
     1561 gtcacctcct tcaaagcacc gtcaaaagaa gaactcgatc atgaatatct ctggcgcgcc
     1621 caccgcgctg tgccccaacg cggcaaaatc ggcatcttca accgctccta ctacgaagaa
     1681 gtcctggtcg tccgcgtcca tcaggatctt ctcaatgccc agcaacttcc caaagagctg
     1741 atcaacaaac atatctggga agaacgctat gaagacatca acaattttga aaaatatttg
     1801 ggtcgcaacg gcatcatcgt catgaagttt ttcctgcacc tctccaaaag tgagcagaaa
     1861 aagcgcttcc tcgaacgcct ggaaatgccc gaaaagaact ggaagttctc catggccgac
     1921 gtgaaagaac gttcctactg gaaggactac cagcaagcct atgaggaaat gatccagaac
     1981 accgccacca agcacgcgcc ctggcatgtc atccccgccg acaacaaatg gttcacacgt
     2041 ttggcggttg ccggcgccat catcgaaaaa ctccactctc tcgacctgca gttccccgaa
     2101 gtcgacgacg acaaaaagaa ggagctggcc caggtccgca aagccctcct ggccggcaaa
     2161 gaatagcggt aagccgagtc ctgtcgtaaa atgccgcctc ttggtagcac agacttcagt
     2221 ctgtgcagcc ttgcgcgggc gacactgcct gccttatggg agaactccac actatgaacc
     2281 acgtagccgc cgtcattgga gccggattcg tcggaaaagc ccacatcgaa gcgctgcgcc
     2341 gcattcctgt ccgcgtcaaa ggcactcttg tcagctccgg cgatcgcagt tccgctgccg
     2401 ccaaagctct cggtcttgaa cgcgcctact ccagtgtcga agaaatcgcc gccgacccag
     2461 aaatcaccgc cgtacacatc tgcaccccca actacgttca cttcggccag tccgccacgc
     2521 tcctgcgggc aggcaagcac gtcatgtgcg aaaagcccct cgccatggac tcgcgcgaat
     2581 ccgcacagct cgtcgcgcta gccaaagaaa ccaatcgtgt cgatggcgtc gcgcacaacc
     2641 ttcgctacta tccgctctgc atccaggcca aagcgctggt cgactccggc gccatcggca
     2701 aagtccgcct cgtccatggc ggcttcctcc aggactggct cacctagccc accgactgga
     2761 actggcgcct cgaatccaaa ctcggcggcg aactccgcgc cgtctctgac atcggcaccc
     2821 attggctgga cctcgttcag tggatcaccg gcgaaaaagt caccgaactc tgcgccgacc
     2881 tcgccaccat cattcctgtt cgccacaaac cgcgcggcca tgtcgaatcc ttctcctccg
     2941 ccaagaatgt cgccaccgac gagatcccca tcgaaaccga cgactacgcc tcggtcctcg
     3001 tgcatctcga acatggcatc cacggcgtgt tcaccgtttc gcaagtcagc cccggccgca
     3061 agtgcgcgct gaattttgaa atcgacggca gcgaaggctc gctctcttgg aacgccgaag
     3121 atcccaacac cctgtggatc ggccgccgca acgaacccaa cggcaaactc atcaaggatc
     3181 cttccctgct caaacccgaa gcccgtacct acgccgccta tcccggtgga cacgccgaag
     3241 gctaccccga caccttcgtg cagctctttc gcgacttcta caactacatc gccgccggca
     3301 acttcagcgc cccacgaaag ttccccacct tccaaactgg ccacgacgaa ctattactct
     3361 gcgaagccat ccaaaaatcc gcccagtccc gctcctggac caaactcgaa tgggactagc
     3421 caaatgacat tcgtggctgc agaagtaaaa ggcctcttca catcggaggg agagccatta
     3481 gagaattgca ccccttccga ctctgaatgt ttccggctct caattcgcgc gtatgttggt
     3541 ccttggggta gcgaagccgc cgagtctttc gatttctatg tatgcacgcc gaaatggctc
     3601 gaacaagaat gcgaaaagcg tggtttcatc gtcggcaggc actatctcat cacgaattct
     3661 ttcgacgctg cccaaatcag agcgctgatc accaagctga tccagcgttt tcaagggagc
     3721 aactggccgg aagtcgccca aaaggttgct cggttcggtt actgggaatt cgaggacctt
     3781 cgcgaatcgg cgtgacaggg atctagcgat ctgcctggaa cc
//
LOCUS       DASTPM010000220         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1160765, whole genome shotgun sequence.
ACCESSION   DASTPM010000220 DASTPM010000000
VERSION     DASTPM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160765"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..908
                     /locus_tag="VFR42_03890"
     CDS             <1..908
                     /locus_tag="VFR42_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU0368323.1"
                     /translation="SGLQQLVRRSGLLRMLPKRLQSMEALLPQLPASETVAEVTPAIG
                     EKRRRVGMLLGCVQREFFPQVNAATARVLAAEGCEVISPGDQPCCGALLVHAGEEDSA
                     LALARYLINVFERAEVDTVVTNAAGCGSNMKEYGHLLRDDPNYAERARAFSAKCRDIS
                     EILAELPSKAKRHPLRLRVAFHDSCHLQHAQGVRIQPRKLLSDIPQLDLLEIPDAAIC
                     CGSAGIYNLVQPNAANALGDRKAALIAPLDPDVVVTGNPGCILQMQASLERAGKRIPV
                     LHTIQLLDTSIRGESLAAIIPTRDS"
     gene            1016..1981
                     /gene="ftsY"
                     /locus_tag="VFR42_03895"
     CDS             1016..1981
                     /gene="ftsY"
                     /locus_tag="VFR42_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU0368324.1"
                     /translation="MITLFGKPEPTLAERLKASVSKTKAALSETVDTIFLGEKRVDPA
                     LLKQLENALLSADLGVGTTREILDSVREKLDRNALSDASQLKAEIKSHIARILNASNG
                     SKAAHPGIVAASPNGKGPRVIFIVGVNGAGKTTSIGKLANLLRKEGSSVILCAADTFR
                     AAAVEQLEIWAQRNGIEVIKQKSGADPAAVVYDAVAAAKARSADAVIVDTAGRLHTKS
                     NLMAELDKMKRTASKVIPGAPHDVLLVLDATTGQNGLAQAREFTSTVGVTGIILTKLD
                     GTAKGGIVVAISRELALPIRFVGTGEQINDLVPFDSETYVNSLFD"
     gene            1985..3094
                     /gene="ribD"
                     /locus_tag="VFR42_03900"
     CDS             1985..3094
                     /gene="ribD"
                     /locus_tag="VFR42_03900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU0368325.1"
                     /translation="MDAHDIRFMEHALALARKGIGLASPNPSVGCVIVREGQIVGEGF
                     HQYEWRDHAEIVALKSAGEKARGATLYVSLEPCNHTGRTGPCSEVIIAAGIHRVVAAM
                     VDPNPVTSGRGFARLRAANVEVACGVLEEDARRLNEAFACWIRSKKPLVTLKSALTLD
                     GQLALPQGKKGRTSAWVTSEESRADVHRMRHASDALLTGIGTILADDPLLTDRSGLPR
                     RRRLLRVVLDTKLRLPPRSRIVQTADDDLLVFTAVSLSGAKARKLQTKGVELVRAPAR
                     RGRMDLSAVLAELGRREVLSVLLEAGPTLNGAALSAGLVHKLRLFYAPKLAGDNGVPF
                     AHMPTVAHSPLPNLRVEQFGPDVALEAYLHDVYRA"
BASE COUNT          669 a          966 c          925 g          671 t
ORIGIN      
        1 gttcagggct gcagcaattg gtgcgccgca gcggtctgtt gcgaatgttg ccaaagcgtt
       61 tgcaaagtat ggaggcgttg ctgccgcaat tgccggcctc cgaaacggtg gcggaagtga
      121 cgccagctat cggtgaaaaa cgccgccgcg tcggaatgtt gctaggttgt gtgcagcgag
      181 agtttttccc gcaggtgaac gccgcgacgg cgcgcgtatt ggcagcggaa ggttgcgaag
      241 tgatcagtcc gggagaccag ccttgctgcg gtgcgttgct ggtccatgcc ggtgaggaag
      301 acagcgccct tgcgttggcg aggtacttga ttaacgtttt tgagcgcgca gaagtggaca
      361 cagttgtgac caatgcggcg gggtgcggct cgaacatgaa agagtacggt catttgctgc
      421 gcgacgatcc gaattacgcc gaacgcgcca gggctttttc cgcgaagtgc agagacatca
      481 gcgaaatact ggcagagctt ccaagcaaag ccaagcgaca tcctctcagg ttgcgggtgg
      541 cgtttcacga ctcttgccac ttgcaacatg cccaaggcgt ccggattcag ccgaggaaat
      601 tattgtcaga cattccgcag ctggaccttc tcgagattcc ggatgcggca atctgctgtg
      661 gttccgccgg aatctataac ctggttcagc cgaacgccgc gaatgcgctt ggtgaccgca
      721 aggcggcact aattgctccg ctcgatcccg atgtcgtggt taccgggaat ccgggatgca
      781 ttctgcagat gcaagcatca ctggagcgtg cagggaagcg cattcccgtt ctgcacacga
      841 ttcaacttct tgacacatcg attcgcgggg agtcgctggc cgcgataatc cccacgcgcg
      901 attcgtaacg ctccgcccgt gtttttccaa tagatttggc ccaatgccgt gccccggcgt
      961 gcggcaaggg cttctgttac acttttctag aactggtatc aagccgaaac cttagatgat
     1021 cacactgttt ggaaaaccgg aaccgacgct ggcagaacgc ctgaaggcca gcgtcagcaa
     1081 gaccaaggcg gcgttatcgg aaaccgtcga cacgatcttt cttggcgaaa agagagtcga
     1141 ccccgcgctt ctgaagcagc tcgaaaacgc actgctttcg gccgatcttg gtgtcgggac
     1201 cacgcgcgaa attctggata gcgtccgcga aaagctggat cgcaacgcgc tttcggacgc
     1261 ttcacaactc aaagccgaaa tcaaatcgca cattgcgcgc attctcaatg cctcaaacgg
     1321 ttccaaagcc gctcatcccg gaattgttgc cgcctcgcct aacgggaaag gaccgcgcgt
     1381 cattttcatt gtcggggtta acggtgctgg aaagaccacc agcatcggga agcttgcgaa
     1441 tctgctgcgt aaggaaggct caagcgtaat cttgtgcgct gcggatacgt tccgcgccgc
     1501 cgctgtcgag caactcgaga tttgggcgca acgcaacggc atcgaagtga tcaagcaaaa
     1561 gtccggcgcc gatccagcgg ctgtggtgta cgatgccgtc gctgcggcga aagcgcgttc
     1621 cgcggatgcc gtcattgtcg atactgccgg ccgcctgcac acaaaatcca accttatggc
     1681 tgagctggac aaaatgaagc gcacggcgtc caaagtgatt cccggcgcgc ctcacgacgt
     1741 gcttcttgtt cttgacgcaa ccaccggtca gaacggtttg gcgcaggccc gcgagttcac
     1801 ttccaccgtc ggtgttaccg gaatcattct gaccaagctc gacggcaccg ccaaaggtgg
     1861 cattgtcgtt gccatttcac gcgagctggc cctgccgatt cgcttcgtgg gcaccggcga
     1921 gcagatcaac gaccttgtcc cgttcgactc cgaaacatac gtcaattcgc tcttcgactg
     1981 attcgtggac gcacacgaca ttcgtttcat ggaacacgcg ctagccctgg cgcgcaaagg
     2041 catcggcctg gccagtccaa atccttccgt gggctgcgtg attgtgcgcg agggccaaat
     2101 cgtcggcgaa ggctttcatc aatatgagtg gcgcgatcac gcggagatcg ttgcgctcaa
     2161 atcggccgga gaaaaggcgc gtggcgcaac cctctacgtt tcccttgaac cttgtaacca
     2221 caccgggcgc accggacctt gctcagaagt gatcatcgct gccgggattc atcgtgttgt
     2281 tgccgcgatg gtggatccta acccggtgac ttccggccgt ggtttcgcac gtcttcgcgc
     2341 cgccaatgtc gaagtggcgt gcggagtgct cgaagaggat gcgcgccgcc ttaatgaagc
     2401 ctttgcctgc tggattcgct caaagaaacc gcttgttacg ctaaagtccg cgctcactct
     2461 cgacggccaa cttgcgttgc ctcaagggaa aaagggccgc acgtccgctt gggtcacgtc
     2521 ggaagaatct cgcgctgacg tccatcgcat gcggcacgcc tcagacgcat tgctcaccgg
     2581 catcggcaca attttggccg acgacccact gctcaccgac cgcagcggct tgccacgtcg
     2641 ccgtcgtttg cttcgcgtag tactcgatac caaactgcgc ctgccgccca gatcgcgcat
     2701 tgtacaaacc gcggacgacg atcttctcgt tttcaccgcc gtgtcgctca gtggggcaaa
     2761 agcccgcaag ctgcaaacca aaggtgttga actcgtccgt gcgccagcaa gacgcggccg
     2821 catggatcta agcgcagtgc ttgccgaact cggacgccgt gaggtcctaa gcgtattgct
     2881 tgaggcgggg cccacgctca atggcgcagc gctttccgcc ggcttggtgc ataaactgcg
     2941 gctcttttac gcgccgaaac tcgccggcga caatggcgtt ccgttcgcgc acatgccaac
     3001 tgttgcgcac agtccactac caaaccttcg agtcgagcag tttggcccag atgtcgccct
     3061 cgaggcctat cttcacgacg tctatcgtgc atgagcctcc gaccgctccc caccacgtta
     3121 aacgccattt ggcggccaaa atgctatcct caattcaatg ttcaccggaa ttatcgagca
     3181 ccttggcatc atagagtcgc tcgcgctgca acccgatggc gggcgcgtca c
//
LOCUS       DASTPM010000221         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_267185, whole genome shotgun sequence.
ACCESSION   DASTPM010000221 DASTPM010000000
VERSION     DASTPM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267185"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VFR42_03905"
     CDS             complement(<1..688)
                     /locus_tag="VFR42_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0368326.1"
                     /translation="MRKLTGSAAGLLLALAAIAPGVRAGTVRGTVNNGTTGKPGAGVE
                     VVLIQLQGGMTPVAKTKSDAGGQFTFDNPGIGAQPMLVRAIYKGVNFHQPLPPGKDSV
                     SVDVFEPTSDTKSISVPSRVVIFQPNGSSLIVGEEYGVKNDSTPPQAYFRTDGNFELL
                     LPEMAELKQIAASGPSGMPVVQAPIDKGKNRYAIAFAFRPGESDVRLSYELPYPGNAA
                     TVKIPTVYGGG"
     gene            complement(685..1050)
                     /locus_tag="VFR42_03910"
     CDS             complement(685..1050)
                     /locus_tag="VFR42_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368327.1"
                     /translation="MFCSLLATSQIASSDWAVLGACFALAVATGLFIFYIQPDASDLA
                     PHRSKLDQLLERRDTIYDNLRDLRFEHRAGKYSESDYETMKNSMEIEAAVVLAEIDQT
                     TDSITRRPRGPKLAQRGAQ"
     gene            complement(1052..1591)
                     /locus_tag="VFR42_03915"
     CDS             complement(1052..1591)
                     /locus_tag="VFR42_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEU0368328.1"
                     /translation="MDNRKAKIGLLALLPLLLMATGAPRLLADQNDRAHKIGDKLKCM
                     CGGCDQSAAKCYHVGGAYSGPCDKAKGMLKEIDAHIAQGKNDEQALQAMIAEYGPLAY
                     VEPPKSGFGLVAWLMPILYLLGGTGAVILIMKRWRKRPAAIAGAAMAAGAGGGHASVQ
                     VTPELLARARELARRETED"
     gene            complement(1615..>3498)
                     /locus_tag="VFR42_03920"
     CDS             complement(1615..>3498)
                     /locus_tag="VFR42_03920"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEU0368329.1"
                     /translation="FFSDNYSVAYVVSHSNRDLSTFFKVAALWSGQEGSLLFWSFLLA
                     VYVFSVLFTYRNKNGELMPYVGVILAGVQLFFLTISNFVASPFRALGVANASGAFDLV
                     ARADGNGLNPVLQYPEMVIHPPNLYSGYTGFTIPFAFALAALLARYPGEKWIHLTRKW
                     TMIAWCFQSMGILLGAHWAYAVLGWGGYWGWDPVENASLLPWLTGTAFLHSVMMQEKR
                     GMMKVWNVWLVFVTFMLCILGTLLTRSGVVSSVHAFAQSSIGNWFVGFLCVVLGVCMA
                     AYFKNKDYLKSENQLDSLVSRESSFLFNNLVLLVACVAVLSGTLFPVFSEWFTGSRIS
                     VGAPFFNKVNIPLGLLLLFLTGVGPLLAWRKTSTDSLLRNFKWPGILGLLGGVIGLVS
                     GFREMFSLICLSLCVFVASTIVQEFYRGAKVVRIRSGASWLASCVDLTLRNTRRYGGY
                     VVHFGMVMIFIGLSGQAFNKEVEKELPIGSTVKIGNYDLLLQSMDQKQEKNYVADRMI
                     VEVLKNDKPVMMLYPERRNFPTNQESGTMVAIYSTLREDLYVVYAGMNPQTNLPTVHA
                     FINPLVKWVWWGGMVVVMGTIVALLPNRVAVVALARAESPVGAPGITGMRPAQVTLRE
                     GHD"
BASE COUNT          779 a         1081 c          961 g          677 t
ORIGIN      
        1 ggccgccgcc atagacggtt ggaattttca ccgtagcggc gttgccggga tacggtagtt
       61 cgtaggataa acgcacgtcg ctctcgccgg gacgaaacgc gaaggcaatg gcgtagcgat
      121 tcttgccttt gtcgatcggc gcttgcacca caggcattcc cgatggcccg gacgccgcaa
      181 tctgcttcag ttcagccatc tcgggcagca atagctcaaa atttccatcc gtccgaaaat
      241 atgcctgcgg cggagtcgag tcattcttta cgccgtactc ttcgccgacg atcaggctcg
      301 atccgtttgg ctgaaaaatg accacgcgcg acggaacaga gatgcttttt gtatcggaag
      361 taggttcaaa gacatcgacg ctgacggaat ctttgccggg cggcagcggc tggtgaaagt
      421 tcacaccctt gtaaatggcg cgcaccagca tcggctgcgc gccaatgcct ggattgtcaa
      481 acgtgaattg gccgccggcg tctgacttgg ttttagcgac cggagtcatg ccgccttgca
      541 actggatcaa aacgacttcc acgccggctc ccggcttgcc agtggtgcca ttgttgaccg
      601 taccgcgtac tgtacccgcg cgaacgcccg gagcaatcgc cgccagcgca aggagtagtc
      661 cggcagcgct gcctgtgagc tttctcattg cgcgccccgc tgcgccagtt tcggtccgcg
      721 cggacgcctg gtgatggagt cggtcgtctg atcaatctcg gccaacacca cggccgcttc
      781 tatttccatg gaatttttca tcgtttcgta atcgctctcg gagtattttc ccgcacgatg
      841 ttcaaaccgc agatcgcgca ggttgtcgta gatggtgtca cggcgctcca gcaactggtc
      901 gagtttcgag cgatggggcg ccagatcgga tgcgtcgggc tgaatgtaga agatgaacag
      961 gccggtggcg acggccagag caaagcatgc ccccagcacg gcccaatccg aggacgcaat
     1021 ttgagacgtt gccagcagcg agcagaacaa cctagtcctc cgtctcgcgg cgggccaatt
     1081 cgcgggcgcg ggcgagcagt tccggagtta cctgcaccga ggcgtggccg ccgcccgctc
     1141 ctgccgccat tgcggcgccg gcgatcgcgg cgggccgctt tctccagcgc ttcataatca
     1201 ggatgaccgc tccagtcccg ccaagcaggt acagaatcgg catcagccaa gctaccaggc
     1261 caaatcccga tttcggaggc tccacatacg ccagcggccc gtattccgcg atcatggcct
     1321 gcaaagcctg ttcgtcgttt ttgccctgcg cgatgtgggc gtcgatttct ttcaacatac
     1381 ctttcgcctt gtcgcagggt ccggaatacg cgccgccgac gtgatagcac ttcgcagccg
     1441 actggtcgca cccgccgcac atgcatttca atttgtcgcc aatcttatgg gcccggtcgt
     1501 tctgatcggc cagcaggcgg ggagcacctg tcgccatcag cagtagcggc aagagcgcca
     1561 gcaatccgat tttcgctttt cggttgtcca ttttcgagtt ccgctgttca gggttcagtc
     1621 gtgcccttcc cgcagggtaa cctgcgccgg acgcatcccg gtgattcccg gtgcgcctac
     1681 tggcgattcc gccctggcca gcgcgaccac tgcgacccgg ttcggcagca atgccacgat
     1741 cgtgcccata acgacgacca tgccgcccca ccacacccac ttcaccagcg ggttgatgaa
     1801 cgcgtgcacc gtcggcagat tggtttgcgg attcatgccc gcgtagacga cgtacaaatc
     1861 ctcgcgcaaa gtggagtaga tcgccaccat tgttccagat tcctgattgg tcggaaaatt
     1921 ccgccgttcg ggatacagca tcatcaccgg cttatcgttc ttgagcactt ccacgatcat
     1981 gcggtctgcg acgtagttct tttcctgttt ctgatccatg ctctgcagca ggagatcgta
     2041 gttgccgatt tttacggtcg agccgatggg cagttccttt tccacttcct tgttgaaggc
     2101 ctggccggat agtccgatga aaatcatcac catgccgaaa tgcacgacgt agccgccata
     2161 acggcgcgtg ttgcgcagcg tcaggtccac gcaggacgcc agccaagaag caccggacct
     2221 aatccgcacc acttttgcgc cgcgataaaa ctcctgcaca atggtgctgg ctacaaacac
     2281 gcaaagggaa aggcatatca gcgaaaacat ttcgcgaaag ccggacacca atccgatcac
     2341 tccgcccaat agccccagga tgccgggcca cttgaaattg cgcagcaaac tatcggtaga
     2401 agtcttgcgc caggcgagca gtggacccac gccggtcaag aacagcagca gcaagcccag
     2461 ggggatgttc actttgttga agaatggcgc gccaacgctg atgcgcgaac cggtgaacca
     2521 ctccgagaat actggaaaca gcgtgccgga gagcaccgcc acgcaggcga ccagcagcac
     2581 caaattgttg aacagaaagc tggactcacg cgagaccagc gaatcaagtt gattctcgct
     2641 cttcaaataa tccttgttct tgaagtacgc agccatgcaa acgcccaaaa cgacgcacaa
     2701 gaaaccgaca aaccagtttc cgatgctcga ctgcgcgaaa gcgtgaacgg aactgacgac
     2761 tccgctgcgc gtcagcagcg tgcccagaat gcacaacatg aacgtcacaa ataccagcca
     2821 gacgttccag accttcatca tgccgcgctt ttcctgcatc atcaccgaat gcaggaacgc
     2881 ggtgccggtc agccacggca gtagcgatgc gttttcgacg gggtcccaac cccagtaacc
     2941 gccccagccg agcacggcat acgcccagtg cgcaccgagc agaattccca tggactggaa
     3001 gcaccacgcg atcatggtcc acttgcgcgt caggtgaatc cacttttcgc caggatagcg
     3061 cgcgagcagc gccgccagcg cgaacgcaaa cggaatcgtg aagcccgtat agcccgaata
     3121 gaggttcgga ggatgaatca ccatttccgg gtactgcagc accggattca gtccgttgcc
     3181 atcagcacgc gcgaccagat cgaacgcgcc actcgcattg gcaacgccca gcgccctaaa
     3241 cgggctcgcc acaaaattgc tgatggtcag gaaaaacagc tgcacgccgg ccagaattac
     3301 gccgacgtat ggcatcagtt cgccattttt gttgcggtag gtgaacagca ccgagaaaac
     3361 ataaacggcc agcaggaaac tccagaacag gagcgagcct tcctggcccg accacaacgc
     3421 cgcaactttg aaaaacgtgg agagatcgcg attgctgtgc gaaaccacgt aggcaaccga
     3481 atagttgtcg ctgaaaaa
//
LOCUS       DASTPM010000222         4750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1016516, whole genome shotgun sequence.
ACCESSION   DASTPM010000222 DASTPM010000000
VERSION     DASTPM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016516"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1619
                     /locus_tag="VFR42_03925"
     CDS             198..1619
                     /locus_tag="VFR42_03925"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEU0368330.1"
                     /translation="MRSTTVPHLDPRSLLEAQRAAFRRGAPHHRQRVAALKSLEAAVI
                     EHREEIVRATNEDFGGRARQETLLLELAPLLDGIRHTRKHLAGWMKPRRVSAGINFFP
                     ARARIIYQPLGVVGILGAWNYQTLLSLSPLVDAIAAGNHAMVKPSELAPKTAEVLQKM
                     ISAIFPPDYIAVVTGDAQLAAEFTSLPFDHLLFTGSTRVGKLVMKAASENLTPVTLEL
                     GGKCPAIVHPEFPLRTAVERILTGKLYNAGQTCLAPDYVFVHESQRDEFVKLASEVAQ
                     QLYPKWSGNPDYTNIISTHHLERLKGYITDAVSRGATKVPLGSQEAPTNTTDRLFTPV
                     VLLDTNDSMQVMREEIFGPILPVLTYRDLEEALEFVNAHERPLALYYFDYNAGRLDHV
                     LQTTISGGVTINDVIYHIAQNNLPFGGVGSSGMGHYHGRAGFETFSKKKSVFLQSRFS
                     TLKFLRPPYGALTDRVIRFLLRT"
     gene            complement(1649..2851)
                     /gene="pcaF"
                     /locus_tag="VFR42_03930"
     CDS             complement(1649..2851)
                     /gene="pcaF"
                     /locus_tag="VFR42_03930"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:TIGR02430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU0368331.1"
                     /translation="MKTVLICDAIRTPIGRYGGALSCVRTDDLAAIPLRALRQKYPSI
                     DWQQLDEVILGCANQAGEDNRNVARMALLLAGLPHNVPGSTVNRLCSSGMEAIGTAAR
                     AIAAGEYDFAIAGGVESMSRAPFVMGKADSAFSRSAEIYDTTIGWRFINPLMKKQYGV
                     DSMPETGENVAEEFRISREDQDAFALRSQQRAAKAQSAGFFAEEIVPVEIAGKKGPTI
                     VSADEHPRPDTTLETLAKLKPAFRTPGTITAGNASGVNDGAAAMVLASENAANRHGLT
                     PRARFLSMAAAGVPPRVMGMGPVPSTQKLLSRLQFSINDFDCIELNEAFASQALACLR
                     QLGIPDDAPHVNPNGGAIALGHPLGMSGARLVLTAIHQLQKTGGRRALATMCVGVGQG
                     VSLAVERA"
     gene            complement(2848..3645)
                     /locus_tag="VFR42_03935"
     CDS             complement(2848..3645)
                     /locus_tag="VFR42_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HEU0368332.1"
                     /translation="MSAAQPAYTTNEIMTVAAARLLKNGAVCFVGIGLPSTAANLARL
                     THAPDIVLIYESGPIGAKPTVLPLSIGDGNLAETADTVVSTPEIFRYWLQGGRVDIGF
                     LGAAQIDRFANINTTVIGDYKKPATRLPGAGGAPEIASCAREVVLVLKQSARAFVKKL
                     DFVTSVGHLDGGDARKRLGLPGKGPVAVVTDLCLMEPDLATKELTVTQLHPGITREQV
                     SKATGWEIHFAGTLKETPAPTSLELETLRDLERRTRLAHGIPLAAST"
     gene            complement(3642..4508)
                     /locus_tag="VFR42_03940"
     CDS             complement(3642..4508)
                     /locus_tag="VFR42_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEU0368333.1"
                     /translation="MAEITTLREAIAALVRDGDTVAMEGFTHLIPFAAGHEVIRQGRK
                     QLTLVRMTPDVIYDQLIGAGCAKKLIFSWGGNPGVGSLHRLRDAVERDWPAPVEIQEH
                     GHGDLSNAYVAGAAKLPFAVIRAYAGSDLPKHNHKIKFIECPFTGEKLAAVPAINPDV
                     TIVHAQKADRKGNVLLWGILGVQKEAILAARKAIVTVEEIVDDLKAWPNACILPAWTL
                     SAICCVPGGAFPSYAHGYYERDNRFYQAWDAISRERETFQNWIKTHILRTPDFSEFRR
                     VLAESAAVGVQQ"
BASE COUNT          974 a         1354 c         1471 g          951 t
ORIGIN      
        1 gcaggggcga acccagccgc ggaactatcg cctgcgagcc cggcccgaca gccatacacc
       61 tccgaaactc ttcgccctcg cttgtccctc gccctccttg tactctgtat cgagccgccg
      121 cctgcgccgg gcagcacaag cctattgccg cgaaacccgc gtcggagcct atggccgcac
      181 cttcaaaaag cccgtcgttg agatcgacta cagtccccca cctggatcct cgctctcttt
      241 tggaggctca gcgggcggcg tttcgccgcg gcgctccgca ccaccggcaa cgagtcgccg
      301 ccctgaagtc gctcgaagct gccgtaatcg agcatcgcga agaaattgtc cgtgcaacga
      361 acgaagattt tggcgggcgg gcacggcagg aaacactctt gctggaactc gcaccattgc
      421 tggacggcat ccgtcacacg cgaaaacatc tcgccggttg gatgaagcct cgccgcgtga
      481 gcgcaggcat taattttttc ccggcccgcg cgcgcatcat ctatcagccg ctcggagtcg
      541 tcggaattct cggtgcatgg aactaccaga cgcttttgtc gctttcccca ttggtcgacg
      601 cgattgctgc aggcaatcac gccatggtca agccgtcgga gctcgctccc aaaaccgccg
      661 aagtcctgca gaaaatgatc tcggctattt ttccgccaga ctacatcgcg gtggtcaccg
      721 gcgacgcaca actcgcggcg gaatttacct cgctgccgtt tgaccacttg ctgttcaccg
      781 gctcgactcg cgtcgggaag ctggtaatga aagccgccag cgagaatctg actcccgtca
      841 cgctggagct gggagggaag tgcccggcga ttgtgcatcc cgaattcccg ctgcgcaccg
      901 cggtcgagcg catcctcacg ggaaagctgt acaacgcagg acagacatgt ctcgcgcccg
      961 actatgtctt cgtccacgaa tcgcaacgcg acgagtttgt aaaactggct agcgaagtgg
     1021 cgcagcagct ttacccaaag tggagcggca atcccgacta cacgaacatc atcagcacgc
     1081 atcacctcga gcgcctgaaa ggttacatca ccgatgccgt ctcgcgtggc gcaacgaaag
     1141 tgccgctcgg gagccaggaa gcaccgacaa acacaaccga cagattgttt acgcccgtcg
     1201 tgctcctcga tacgaatgat tccatgcaag tcatgcgaga agaaatcttt ggcccgatac
     1261 ttccggtact gacgtatcga gatctagagg aagctctgga attcgtgaac gcgcacgaac
     1321 gcccgctcgc gctctactat ttcgactaca acgctggccg cctagaccac gtactgcaga
     1381 ccacgatctc cggtggagtc acgatcaacg atgtgatcta ccacatcgcg cagaacaatt
     1441 tgccttttgg gggagtcggc tccagcggca tgggccacta ccacggacgc gccggtttcg
     1501 agaccttctc caagaaaaag agcgtttttc tgcagtctcg tttcagcacg ctcaaattct
     1561 tgcgccctcc ttacggtgca ctcaccgatc gcgttatccg cttcctgctc cgcacgtaag
     1621 cagcgctcga caaaaggaac tcgggaactc aggctcgttc gaccgccaag gaaacgcctt
     1681 ggcctactcc tacacacatg gtggcgagag cgcggcggcc accggtcttt tgcagttgat
     1741 gaatcgcggt gagcacgagg cgtgcgccgc tcatgcccag aggatgacca agtgcgatgg
     1801 ctccgccgtt gggattgacg tgcggcgcgt catcaggaat gcccagttgg cgcagacacg
     1861 ccagggcctg gctggcgaag gcttcgttca attcgatgca atcaaaatcg ttgatggaaa
     1921 actgaaggcg cgaaagaagc ttttgcgtgg acggcacggg acccatgccc ataacgcgcg
     1981 gcgggacgcc ggctgccgcc atactgagga agcgcgcgcg cggtgtcaga ccgtgacgat
     2041 ttgcggcgtt ttcggaggcc aggaccattg cggctgcgcc atcgttcaca ccggaagcat
     2101 tgcccgcggt gatggtgccg ggcgtgcgaa aggctggctt gagtttggcg agagtttcga
     2161 gcgtagtatc ggggcgcgga tgctcgtcgg cggaaacgat ggtggggccc tttttaccag
     2221 cgatttcgac ggggacgatt tcctcagcaa aaaatcctgc agattgggct ttcgccgcgc
     2281 gctgctggct ccgcaaagcg aaggcgtcct gatcttcgcg gctgatgcga aattcttccg
     2341 cgacgttttc gccggtctcc ggcatggaat ccacgccgta ttgttttttc atgagcgggt
     2401 tgatgaaacg ccagccgatg gtggtgtcgt aaatttcggc cgagcgcgaa aaggcggaat
     2461 cggctttgcc catgacgaag ggtgcgcgcg acatggactc aacgcccccg gcaatggcga
     2521 aatcgtattc gccggcggca atcgcccgcg ccgccgtacc gatggcctcc atgccggaag
     2581 agcacaggcg gttgaccgtt gagcctggaa cgttgtgggg aagtccggcc agaagcagag
     2641 ccatgcgagc gacgttgcga ttgtcttcgc cggcttgatt ggcgcagccg aggatgactt
     2701 cgtcgagttg ctgccagtcg atgctgggat atttctgtct aagagcgcgc agcgggattg
     2761 cggcgagatc gtcagtacga acgcaagaaa gcgcgccgcc atagcggcct atcggtgtgc
     2821 ggatcgcgtc gcaaatcaga actgtcttca tgttgaggcc gctagcggaa tgccgtgcgc
     2881 gagtctagtg cgccgttcaa ggtcgcgcag cgtttcaagt tcgaggctgg tcggagcggg
     2941 agtctctttc agagtgcctg cgaaatgaat ttcccagcca gtggctttgc tgacttgctc
     3001 gcgagtgatg ccgggatgga gttgcgtgac ggtgagttcc ttggtggcca gatctggctc
     3061 catcagacaa aggtcggtga caacggcgac gggacccttg ccggggaggc caaggcgttt
     3121 gcgtgcgtca ccgccgtcga gatgaccgac ggaagtgacg aagtcgagtt tcttgacgaa
     3181 agcgcgagcg gattgtttga gtacgaggac gacttcacgc gcacaactgg cgatctcggg
     3241 agcaccaccc gctccgggaa ggcgcgtggc gggctttttg taatctccga tcaccgtggt
     3301 gttgatgttg gcgaagcgat cgatttgcgc ggcgccgagg aaaccgatat cgacgcggcc
     3361 accttgtagc caatagcgaa aaatttccgg ggtggagaca acggtgtcag cagtttcggc
     3421 gaggttgccg tccccgatgg agagcggaag gacggtaggt ttggcgccga tggggccgga
     3481 ttcatagatt aaaacgatgt ccggcgcatg ggtaaggcgc gcgagatttg ctgcggtgga
     3541 aggaagccca ataccgacga aacagactgc gccgtttttg agaaggcgag cggcggcaac
     3601 ggtcatgatt tcgttggtgg tataagcagg ctgggcagca ctcattgttg gacacccacg
     3661 gcggcggatt cggcgaggac acggcgaaac tcgctgaagt ccggcgtcct caggatgtgc
     3721 gttttgatcc aattctgaaa agtttcgcgc tcgcgggaaa tagcgtccca ggcctgatag
     3781 aagcgattgt cgcgttcgta gtagccgtgc gcgtaagagg ggaaagcgcc gccgggaacg
     3841 cagcagatgg cggaaagcgt ccatgccggg agaatgcagg cgttaggcca ggctttcagg
     3901 tcgtcgacga tttcttcgac ggtgacgatg gcttttcttg cggcgagtat cgcttctttt
     3961 tgcacgccaa gaattcccca gagcaagacg ttgcctttgc gatcggcctt ctgcgcatga
     4021 acgatggtga cgtcgggatt gatcgccggg acggcagcga gtttttctcc ggtgaacggg
     4081 cactcgatga atttgatttt gtggttatgt ttgggcaaat cggaaccggc ataggcgcga
     4141 attacggcaa acgggagttt ggcagctccg gcgacgtaag cattggataa gtcgccgtga
     4201 ccgtgttcct ggatttcgac cggcgccggc cagtcgcgtt ctacggcgtc gcgcagacgg
     4261 tgcagggaac cgacgccggg attgccgccc caggagaaaa tcaatttttt tgcgcagccg
     4321 gcgccgatga gctggtcgta gatcacatcg ggtgtcatgc ggacgagagt gagctgcttg
     4381 cggccctggc ggatgacctc gtggccggcg gcaaaaggga tcaggtgcgt gaagccttcc
     4441 attgcgaccg tgtcgccatc gcgcaccaga gcggcaattg cttcacgaag agtggtgatt
     4501 tcagccattg gaagttcgct gctgtcagtg cggcaatatt tcagtccgca tgcattctat
     4561 ctcaatgata cacgcaggag cgcacgtgcg ggacgggcac catcgtttgc gctgcataaa
     4621 caaggcttcc gccagcgaaa gcgcgcggcc taaatgtgga gcgtacgcac cagcgcactc
     4681 actccgttcc acaaaatctg cacgccgatg cacattcggc aataacgccg acaggcgtag
     4741 gatcacgtcc
//
LOCUS       DASTPM010000223         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_958966, whole genome shotgun sequence.
ACCESSION   DASTPM010000223 DASTPM010000000
VERSION     DASTPM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958966"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VFR42_03945"
     CDS             complement(<1..959)
                     /locus_tag="VFR42_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0368334.1"
                     /translation="MSYQLNRRSFIVAGGLTALASTRVLGANDTLRIGVLGSGGRMRD
                     LLNAANSVGGYQLVAINDVYGPHREQVKERFGPTATLHGDYREVLQQPIDAVFIASPD
                     HWHVRMAVDALAAGKDVYLEKPVTHTLEEGAALTRAVRSGKQILQCGMQQRSWSHFRD
                     AVELIQGGSLGRVPQVRTYWWQNYLAAWPMKPMEMDKLDWKQWLGGAPEQAFTEEKFY
                     HWRWFWNFGGGAMTDLFTHWIDVVHWAMKADQPSEAQMLADKYVFQSWDCPDTLQAAL
                     RYPAFDVVYEGSMASSIDDGGLEFRGTEATLKINRSGFAVHREG"
     gene            1191..1784
                     /locus_tag="VFR42_03950"
     CDS             1191..1784
                     /locus_tag="VFR42_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HEU0368335.1"
                     /translation="MQKIQIAVGSTRRPKLSAVSEALREFGPLLAPNAEFEIIGVEVE
                     SGVSHTPANRAELMSGARQRAEAVQRLAQEKSESWRYFVGLEGGLDLIEEPAGRRVFL
                     ESWAYVSDGRTGHYGRSGGVEIPAALAREVLENGVELSLAIDRFAGAVGIRDAQGAWG
                     VLSAGLISRTEAFRVAVVAAFAPFYNAKMYRAASSAS"
     gene            1840..2460
                     /locus_tag="VFR42_03955"
     CDS             1840..2460
                     /locus_tag="VFR42_03955"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF003296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU0368336.1"
                     /translation="MDIVKGNMGWIEVVVGPMFSGKSEELIRRLRRAEIARQRVQIFK
                     PAIDERYAANEIISHSGLGIASDNIHKAGEILDKLQPRTEVIGIDEAQFLGDEVVDVC
                     TKLANLGKRVIVTGLDTDYRGRPFEPMPRLLAIAEEITKLLAICVRCGNPAVHTQRLV
                     ESEELIVVGASGMYEARCRRCFEPNPEAAREKKETPLLIRNRAAHS"
BASE COUNT          503 a          908 c          655 g          466 t
ORIGIN      
        1 ccctcgcgat gcaccgcaaa gccgctgcga ttgattttca gcgtcgcttc cgttccgcgg
       61 aattccagtc cgccgtcgtc aatcgacgac gccatcgagc cctcgtacac cacgtcaaac
      121 gccggatagc gcagcgccgc ctgcagcgtg tccgggcaat cccagctctg gaaaacatac
      181 ttgtcggcca gcatctgtgc ctcgctcggc tggtccgctt tcatcgccca gtgcaccacg
      241 tcaatccagt gcgtaaacaa atcggtcatc gccccgccgc caaaattcca gaaccaccgc
      301 cagtgataaa atttttcctc ggtgaacgcc tgttccggcg cgccgcccag ccactgcttc
      361 caatccagct tgtccatctc catcggcttc atcggccacg ccgccaaata gttttgccac
      421 caatacgttc gcacctgcgg cacccgtccc aggctgccgc cctggattag ttccaccgcg
      481 tcgcgaaagt ggctccagct gcgctgctgc ataccgcatt gcagaatctg ctttccagag
      541 cgaaccgcgc gcgtcagcgc cgcaccctcc tccagcgtgt gcgtcaccgg cttttccagg
      601 tacacatctt tgcccgcggc cagcgcatcc accgccatcc gcacgtgcca atgatccggt
      661 gatgcgataa acaccgcatc gatcggctgt tgcagcacct cgcgataatc accatgcaag
      721 gtcgccgtcg ggccaaagcg ttccttcact tgctcgcgat gcggtccata cacatcgttg
      781 atcgccacca gttgatagcc gcccacgctg ttcgcggcgt tcaacaggtc gcgcatccgc
      841 ccgccggatc ccagcactcc aatccgcagc gtgtcgttcg cacccagcac ccgcgtggac
      901 gccagcgcgg tcaatcctcc cgccacaata aaactgcgtc gattcagttg gtagctcatg
      961 cccgacatgt tagcgactcg cgccgcagta gggaagcgcc ctcatccagc aacgccgtca
     1021 tcctgaggct acccgacaag ggtagccgaa ggatctcaac cgttggcctg gcacgacgaa
     1081 acaggtctcc acgcttgtct gcacctcgcc tcagatattt ttcctcagct aacattgttt
     1141 tccacctggc acttcgcaca gggaagccat tacaatcgcc tcgatcccca atgcaaaaaa
     1201 tccaaatcgc cgttggcagc acccgccgcc ccaagctctc cgccgtcagc gaagccctcc
     1261 gcgaatttgg cccccttctc gcgcccaacg cggaattcga aatcatcggc gtagaggtag
     1321 aaagtggcgt cagccatacg cccgccaatc gcgccgaact gatgtccggc gctcgccagc
     1381 gcgccgaagc cgtccagcgt ctagcccagg aaaaatccga atcctggcgc tattttgtcg
     1441 gcttggaagg cgggctggat ctgatcgaag agccagccgg ccgccgcgtc ttcctcgaaa
     1501 gctgggccta tgtctccgat ggccgcactg gccattacgg ccggtccggc ggcgtcgaga
     1561 tccccgcagc cctcgcccgc gaagtcctcg aaaacggtgt cgagctttcc ctcgccatcg
     1621 accgcttcgc gggcgccgta ggcatccgcg acgcccaagg cgcttggggc gtgctctccg
     1681 ccggcctcat cagccgcacc gaagcctttc gggtagcggt ggtcgccgcc ttcgctcctt
     1741 tttataacgc aaagatgtac cgcgcggcct catctgcgtc ataataagag caacgtcatt
     1801 cggtcggcat gagcagcgta cgtccccagg gagacttcag tggatatcgt caaaggcaat
     1861 atgggctgga tcgaagtggt cgtcggcccc atgttttcgg gaaaaagcga agaactgatc
     1921 cgccggctgc gccgcgcgga aatcgcccgc cagcgtgtgc aaatcttcaa gcccgcgatt
     1981 gacgagcgct acgccgccaa cgaaatcatc tctcactccg gcttgggcat cgcctccgac
     2041 aacatccaca aagccggcga aatcctcgac aagctgcagc cccgcaccga agtcatcggc
     2101 atcgacgaag cgcaattcct cggcgacgaa gtggtcgacg tctgcaccaa gctggccaac
     2161 ctcggcaagc gcgtcatcgt cactggcctg gacaccgact accgtggccg cccgttcgag
     2221 cccatgcctc gccttctcgc catcgccgaa gaaatcacca agctcctggc catctgcgtg
     2281 cgctgcggca accccgccgt gcacacccag cgcctggttg aaagcgaaga gctgatcgtg
     2341 gtcggcgcct ccggcatgta cgaagcccgc tgccgccgct gcttcgaacc caacccggaa
     2401 gccgcccgcg aaaaaaaaga gaccccgctt ctgatccgta atcgagcagc gcattcctaa
     2461 gtagcatagg cttcagcctg tgctccgacg atgaagcccg tcattgacgg cgaccgcgat
     2521 ctcggaccta cc
//
LOCUS       DASTPM010000224         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1333752, whole genome shotgun sequence.
ACCESSION   DASTPM010000224 DASTPM010000000
VERSION     DASTPM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333752"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..>2719
                     /locus_tag="VFR42_03960"
     CDS             466..>2719
                     /locus_tag="VFR42_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0368337.1"
                     /translation="MRRLLALLILGSPVVSLALKAQSNSGELRLKLTDPSGSAVKGSI
                     ELVCDANQFRQTYVTDNSGAAIAKRLAFGVYEVGVRQAGFAPYHSLVEIRSAVPEELR
                     ISLGLASVNTSVTVNDTETLVDPHRTGSVNRIGSETLEARPTALPGRSVVDLVNSQPG
                     WLYEGNAVLHPRGSEYHTQFVVDGVPLTDNRSPSFGTEIEADDVQSLSIYTANIPAEY
                     GRKLGGVVEVTTAKDPRLGFHGKAALTGGSFATGSAYLLGEYARPKDAVSVSLDGART
                     DRFLNPPVAQNFSNQATTADFSARYERDFSESDRIGLTVRHGLSRFEVPNEQLQQAAG
                     QRQDRANSETMGILSYQHIFSPNVLGDLRLMVRDDSDTLGSNPLSTPIIAFQDRGFRE
                     EYLKGSVSAHHGRHEWKVGVEADFTSIRENFRDVITDAAQFAPGTQASFRFAGTGRDL
                     EQSGFVQDFIRLGKWTASAGLRWDHYQLLVNQNAVSPRLGIARYVPALDLVLHASYDR
                     VFQTPAFENILLSSSAEVVALNPNVLRLPVEPSLGNYYEAGATKGFAGRVKLDVNYFR
                     RNAGNFADDDQLLNTGVSFPIAFRKSSIYGAEGKLEIPHLGNLSGFVSYSYMVGSTYL
                     PVTGGLFLGDDAANALSKTVGRFWDTQDQRHTARTRFRYVLTPRLWAALGAEYGSGLP
                     VAFEGSVTEAIAQYGPEIIDRVNFARNRVKPSFSLDASAGAEIWKHDSLQVRVQADAA
                     NLTNRLNLIDF"
BASE COUNT          546 a          815 c          793 g          565 t
ORIGIN      
        1 gccaccgaac tggcggaacg tcgaaagatt cctctcgccg cactgtttgc ggccaacgaa
       61 tcggtgccgg caaatagacc tggttgagga tcgaagaact aggtccggtc ggacctaatg
      121 cggagcaagg aaagtaaaaa aactcggtcg cgcgacatcg ctatgcgtcg aggtctaccc
      181 aatccttccg atgttttggc ggtcgcttac gtttatcggg gatcactcga gtggaagcgg
      241 gcgcaatgcc agaaacccgc gccctgcggc gcgcctcctt gcctgcctcg aagcgcttct
      301 gtttgcgtct tttcacgaca ccgccactcc acatgatatc tctcatgcgc cgcgttgcat
      361 ggtcaactgt agtctgggcg gtgcacgccc aaggcgcaac ccgaatgaac gcgcgcggag
      421 tctaaatgtt gcgtagctgc ccgtcaaagc ggatgaccat ctgaaatgcg aaggctcctg
      481 gccctcttga ttcttggctc gccggtcgtt tcccttgcgc tcaaggcgca aagcaactcc
      541 ggtgagctgc gcctgaaact gaccgacccc tccggctcgg cggtgaaggg ctcgatcgag
      601 ctggtctgtg atgcgaacca gtttcgccag acctacgtta cggataactc cggcgccgcc
      661 atcgcaaagc ggctggcgtt tggcgtctac gaggttggcg tgcggcaagc gggctttgcg
      721 ccgtatcaca gtcttgtgga aattcgctct gccgtcccgg aggagctccg gatttcgctt
      781 ggcctggcgt ccgtgaatac atcggtgacc gtgaatgata ccgagaccct ggtggatccc
      841 cacagaaccg gcagcgtgaa tcgaatcggc tccgaaactt tggaggcccg gcccaccgct
      901 ctgcccggac gctcggtcgt tgacctcgtc aactctcaac ctggttggct ttacgaaggc
      961 aacgcggtgt tgcatccgcg aggctccgaa taccacacgc aattcgtggt ggatggtgta
     1021 ccgctcacag acaatcgctc accgagtttc ggcaccgaaa tcgaagcgga tgatgtgcag
     1081 tcgctgagta tctacaccgc gaacattccc gcggaatatg gccggaagct gggcggcgtg
     1141 gtcgaagtga ccaccgcgaa agacccgcgg ctgggctttc atggtaaagc ggcgctgacc
     1201 gggggaagtt ttgctacggg cagcgcttat ctcctgggcg aatacgcgag gccgaaagac
     1261 gcagtcagcg tttcgctgga cggcgctcgc accgatcgat tcctgaatcc gccggtggct
     1321 caaaacttct cgaaccaggc aaccacggcc gatttctccg cgcgctatga acgcgatttc
     1381 agcgaaagcg atcgcatcgg tctcaccgtg cgccacggcc tttcacgatt tgaggttccg
     1441 aacgaacaat tgcaacaagc cgcgggacag cggcaagacc gtgccaactc agagaccatg
     1501 ggaatattgt cgtatcagca tattttttca ccgaatgtgc tgggcgacct tcgactgatg
     1561 gttcgggacg actcggatac tctcggctcg aatccccttt ccacgccgat aatcgcgttc
     1621 caggacagag gttttcgcga ggaatacctc aagggcagcg tttccgcgca tcacggaagg
     1681 cacgaatgga aggtcggcgt cgaagccgat ttcacatcga tccgcgagaa ctttcgcgac
     1741 gtgatcacag acgcggcgca attcgcgcct ggtacgcagg caagcttccg tttcgcgggc
     1801 accggccgcg atctggagca atccggtttc gttcaggact tcattcgcct ggggaaatgg
     1861 acggcaagcg ccggcctgcg ctgggatcat taccaactgc tcgtaaacca gaacgcggtc
     1921 agcccgcgcc tgggaatagc gcgatacgtg cccgccctcg atctggtgtt gcacgcctcg
     1981 tatgaccgcg tgtttcagac gcccgctttc gagaatatcc tgctgtccag ttccgcggag
     2041 gtggtcgcgc tgaacccgaa cgtcctgcgc ctcccggttg agccttcgct tggaaactat
     2101 tatgaagccg gagctacaaa aggattcgcc gggcgcgtaa agctcgacgt caactatttc
     2161 cggcgaaacg ccggcaattt tgccgacgac gatcaattgc tcaataccgg cgtgagcttc
     2221 cccattgcct tccgcaagtc ctcgatttac ggcgcagagg gaaagctgga gattccgcac
     2281 ctcggaaatc ttagcggatt tgtcagctat tcctacatgg tggggtccac gtatttgccg
     2341 gtgaccggcg gccttttcct tggcgatgat gccgcgaacg cactcagcaa gaccgtgggg
     2401 cgtttttggg atacgcagga tcagcgccac accgcccgca cgcgtttccg ttacgtactc
     2461 actccgcgtt tgtgggccgc gcttggcgcg gaatatggaa gcggtctacc cgtcgccttc
     2521 gaaggaagcg tgaccgaggc catcgcacag tatggccccg aaattatcga ccgcgtcaat
     2581 ttcgcccgaa atcgcgtgaa gccatctttc tcgctcgatg catccgcggg cgcggagatt
     2641 tggaagcatg attccctgca ggtccgcgtg caggccgatg cggctaacct gaccaatcgc
     2701 ctgaatctga tcgattttg
//
LOCUS       DASTPM010000225         6026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1420617, whole genome shotgun sequence.
ACCESSION   DASTPM010000225 DASTPM010000000
VERSION     DASTPM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420617"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..620
                     /gene="rplF"
                     /locus_tag="VFR42_03965"
     CDS             75..620
                     /gene="rplF"
                     /locus_tag="VFR42_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU0368338.1"
                     /translation="MSRIGNKPIPVPAGVKIAMVSDKIDVQGPKGKLSVAIPRGISFE
                     QKDGVLNVKRESDEHRAVHGLARALVANAVHGVTQGFKKEMDIVGVGYRAELKGKAVA
                     FALGKSHPVEFKVPEGIQIAVDKQTHLVISGADKGQVGQVAADIRSLRPPDPYKQKGI
                     RITGERLKKKAGKAGAKAGAA"
     gene            697..1077
                     /gene="rplR"
                     /locus_tag="VFR42_03970"
     CDS             697..1077
                     /gene="rplR"
                     /locus_tag="VFR42_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU0368339.1"
                     /translation="MASVPVRRLSRDLKRQRVHQRVRMTVSGTAERPRLCVYRSLDHI
                     YAQVIDDRAGKTLVSASSADKDTKKNLKGGGNVAAAKVVGKTVAERAKAAGVSKVVFD
                     RGGYKYHGRVKALADAAREAGLQF"
     gene            1124..1657
                     /gene="rpsE"
                     /locus_tag="VFR42_03975"
     CDS             1124..1657
                     /gene="rpsE"
                     /locus_tag="VFR42_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU0368340.1"
                     /translation="MLEVNPSELKDDVIAINRVTKVVKGGKNLSFSALVVVGDNHGHA
                     GFGMGKAREVPMAIKKGIEQAKKNLIQLNLKGSTIPHQVIGRYGSAQVLLKPAPEGTG
                     VIAGGPVRKVMQVAGIHNVLTKSLGTSNPHNVVKATFAALLGLKDAAQVAETRTKTVE
                     EITGREKKTEQAAGEAR"
     gene            1654..1872
                     /gene="rpmD"
                     /locus_tag="VFR42_03980"
     CDS             1654..1872
                     /gene="rpmD"
                     /locus_tag="VFR42_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU0368341.1"
                     /translation="MTAQKKTGTQSQGKGGTIKVKWVVSFIACTDDMRQTIRGLGLRR
                     LQQVVERQDTPETRGMIHKVRHLVEVVD"
     gene            1934..2374
                     /gene="rplO"
                     /locus_tag="VFR42_03985"
     CDS             1934..2374
                     /gene="rplO"
                     /locus_tag="VFR42_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU0368342.1"
                     /translation="MGLETLRPPRGSRHRKVRVGRGIGSKLGKTSGSGNKGQKSRRGY
                     SRRRGFEGGQMPLHRRIPKRGFNNPFASSFAVINLEELNVFPAGETVTPDLLRAHGFV
                     RTRQQIKVLGDGELKSKLVIHAHAFSASAKEKIAKAGGSFEVLS"
     gene            2454..3860
                     /gene="secY"
                     /locus_tag="VFR42_03990"
     CDS             2454..3860
                     /gene="secY"
                     /locus_tag="VFR42_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU0368343.1"
                     /translation="MNRFLQALANLFRIEDLRKRVLFTLALLAVYRIGAHIPTPGINS
                     ELLNQIFAQAQGTALGIFDLFSGGNFRRLTIFALGIMPYITSSIILQLMTVVFPYLER
                     LQKEGELGRRKITQYTRYLTIVLSVIQSLSTASLLQSQSLNGASVVYHPGWGFKLMTV
                     LTLTTGSAFIMWLGEQISERGIGNGMSLIIFVGIVVGLPRAMVDLWEKSRTQAWGVFT
                     PMAIILLLVLMVAVVAFIVLVEQGQRRIPVQYAKRVVGRRVMGGQSTYLPLRVNSGGV
                     IPPIFASSLLAFPATLGLLLGSRWVFVKDFADKVKWGEPLYTLLYVTLILLFAFFYVG
                     IVFNPTELADNMRKNGGFIPGIRPGRNTSEYVSRILKRLTLIGAVYLALVCLLPEWMI
                     AGIHLNHLPTWLGGSWFDRHFPQWILNGLGVNFYFGGTSLLIVVGVAMDTVQQLEAQL
                     VMRNYEGFARKGRIRGRR"
     gene            3889..4581
                     /locus_tag="VFR42_03995"
     CDS             3889..4581
                     /locus_tag="VFR42_03995"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU0368344.1"
                     /translation="MTSDIRPHEKLDRAVIFLGPPGAGKGTQAKELARRFSAPHLSTG
                     DMLREHVAQGTSLGQRAQPIMSRGELVPDSIVLRMVAERIERPDCTHGFVFDGFPRTV
                     AQAQWLGELLKQHGFKQPLVVHMVIDPALLLRRITGRRMCKVGGEIYNVYERPPKVEG
                     RCDNDGGELEQRADDREEIVGPRLAAYEKQTAPLAAYYRRLGRLRDIDASKSVDDVAA
                     SIVAVVCDAPKQ"
     gene            4613..5386
                     /gene="map"
                     /locus_tag="VFR42_04000"
     CDS             4613..5386
                     /gene="map"
                     /locus_tag="VFR42_04000"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU0368345.1"
                     /translation="MAIHLRSADEIEKMHRAGLIVHEVLNTLRSSVRPGMTTMDLEKI
                     AEEKIAGKPGHPAFKGYRGYPCVLCTSVNSEIVHGIPSPKRKLKEGDIVSIDFGMEVD
                     GYFADSAVTVPVGKIKPELQKLLDVTRDSLDRAIEQMRAGNRLGDVGHAVQSLVEENG
                     FSVVREFVGHGIGTKMHDEPNLPNYGEPGRGARLQEGMVIAVEPMVNAGRHEVRMRDE
                     WVAETADGSPSAHFEHTVAVTSNGPLILTRPKEMTGPSW"
     gene            5495..5755
                     /gene="infA"
                     /locus_tag="VFR42_04005"
     CDS             5495..5755
                     /gene="infA"
                     /locus_tag="VFR42_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0368346.1"
                     /translation="MAKHEDDKKKGDSGNPKEDAIEVMATVIEPLPNAMFRVELENKH
                     QVLAHVSGKMRKNFIRILAGDKVAVELSPYDLTRGRIVYRYK"
     gene            5859..5972
                     /gene="rpmJ"
                     /locus_tag="VFR42_04010"
     CDS             5859..5972
                     /gene="rpmJ"
                     /locus_tag="VFR42_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU0368347.1"
                     /translation="MKVRASVKKICDKCKVIHRRGVVRVICVNPKHKQRQG"
BASE COUNT         1258 a         1659 c         1875 g         1234 t
ORIGIN      
        1 tgggcggcga attgctttgc gaagtctggt aggttttggt tttttaaaca aggtttttaa
       61 aggttgaatt aaaaatgtca cgtatcggaa acaagccaat tccagtgccc gcgggcgtaa
      121 agattgcgat ggtgtcggac aagattgatg tccaggggcc caagggcaag ctcagcgtcg
      181 ccattccgcg cggaatttcg ttcgagcaga aagacggcgt gctcaacgtc aagcgcgaat
      241 cggacgaaca ccgcgccgtg cacggccttg ctcgcgcgct ggtcgccaac gcggttcacg
      301 gcgtcacgca gggcttcaag aaagagatgg acattgtcgg tgtcggctac cgcgcggaac
      361 tcaagggcaa agccgtagcg ttcgcgcttg gcaaatcgca cccggtcgaa ttcaaagttc
      421 cggaaggcat ccagatcgcc gtggacaagc agacccacct tgtcatcagc ggcgcggaca
      481 aaggccaagt cggccaggtg gcggcggata tccgttcgct gcgtccgccc gatccgtaca
      541 agcaaaaggg aattcgcatt accggcgagc gcttgaagaa gaaagcaggc aaggctggag
      601 ccaaggctgg cgcagcatag ccgcgccgct ggagagggtt gctgccagtc gttttggcca
      661 ggaatttgaa ggaccaggaa ttgaaggaga agaacagtgg catccgtacc ggttcgcaga
      721 ctatcgagag atctcaagcg ccagcgcgtc catcagcgcg tgcgcatgac ggtgtcgggc
      781 accgccgagc ggccgcgcct gtgcgtgtat cgttcgctgg atcacattta tgcgcaggtg
      841 attgacgacc gcgcgggcaa gacgctggtt tccgcgagtt ccgcggacaa ggacaccaaa
      901 aagaatttga agggcggcgg caacgtcgca gcagccaaag tcgtcggcaa aactgttgcc
      961 gaacgcgcca aggctgccgg agtttccaag gttgtgttcg accgcggcgg atacaagtat
     1021 cacggccgcg tcaaggcgct cgcggatgcg gcgcgggaag cggggttaca gttctaagcc
     1081 tatgtcgaag caaattttga gggacagtct tgcggtgcgg cgcttgctcg aagtcaatcc
     1141 ttccgagttg aaggacgacg tcatcgccat caaccgcgtc accaaggtgg tcaagggcgg
     1201 caagaatctg agcttctccg cgctggtcgt cgttggcgac aatcacggcc atgccggatt
     1261 tggcatgggc aaagcgcgcg aagtgccgat ggccatcaag aagggcatcg agcaggcgaa
     1321 aaagaatttg attcagctga acctgaaggg ctctaccatt ccgcatcagg tcattggccg
     1381 ctatggttcg gcgcaggtgc tgctgaagcc agctcccgaa ggtaccggcg tgatcgccgg
     1441 cggtccggtg cgcaaagtga tgcaggttgc gggcattcac aacgtgctga ccaagtcgct
     1501 cggcacttcc aacccgcaca acgtcgtcaa ggctacgttt gcggcattgc tgggattgaa
     1561 agatgccgct caggtggcgg aaacccgcac caagacggtt gaagagatta ccggccgcga
     1621 aaagaaaact gagcaggccg caggggaagc aagatgacgg cacagaaaaa aactggcacg
     1681 cagagccagg gaaaaggcgg cacgatcaaa gtaaaatggg tggtcagctt tatcgcctgc
     1741 acggatgaca tgcgccagac cattcgcggg ctcggcttgc gccgcctgca gcaggttgtt
     1801 gagcgccagg atacgccgga aactcgcgga atgattcaca aagtgcgcca tctagtggaa
     1861 gtggtggact aaattcatgg ctaagaaaaa agaagagtcg actgcttcgc agtcgcggat
     1921 caaaaagacg accatcgggc tcgaaacgct gcgcccgccg cgtggctcgc gacaccgcaa
     1981 agtgcgcgtc ggccgaggca tcggctccaa gctgggcaag acgtccggct ccggcaacaa
     2041 gggacaaaag tcgcgtcgcg gttattcgcg ccggcgcggt tttgagggcg gccagatgcc
     2101 gttgcatcgc cgtattccca agcgcggatt caacaatccg tttgcgtcga gtttcgccgt
     2161 gatcaatctg gaagaactca acgtttttcc ggctggtgaa acggtaacgc cagatctgct
     2221 tcgcgcgcat ggttttgtcc gcacacggca gcaaataaaa gttttgggtg atggcgaact
     2281 gaagtccaag ctggtcatcc acgctcacgc cttcagcgcc tccgccaagg aaaagatcgc
     2341 caaggccggc ggctcgttcg aggttttgtc ttaacggttt cgtattgctg ggtcttcaga
     2401 tccgtgcagt tgtagttccg ggcttttagc tcgattggtt tacaagggta cgcatgaatc
     2461 gcttcttaca agctctcgcg aatctcttcc gcatcgaaga cttgcggaag cgcgtcctgt
     2521 ttacgctggc attgctagcg gtgtaccgca tcggtgcgca cataccgact cccggcatca
     2581 acagcgagtt gctcaaccag atctttgcgc aagcgcaggg caccgctctc ggcatcttcg
     2641 atcttttcag cggcggtaat ttccgccgtt tgacgatctt cgctctgggc atcatgccgt
     2701 acatcacttc gtccatcatc cttcagttga tgacggtggt attcccgtac ctcgagcgct
     2761 tgcagaaaga gggcgagctc ggccgccgta aaattacgca gtacacgcgc tacctgacca
     2821 tcgtcttgag cgtgatccag tcgctctcca ccgccagctt gctgcaaagc cagagcctca
     2881 atggcgcctc ggtggtttac cacccgggct ggggcttcaa gctgatgacc gtgctgaccc
     2941 tcaccaccgg ctccgctttc atcatgtggc tcggtgaaca gatcagtgaa cgcggcatcg
     3001 gcaacggtat gtcgctcatc atttttgtcg gcatcgtggt tggcctgccg cgtgccatgg
     3061 tggacctctg ggaaaagtcg cgcacccagg cttggggcgt tttcacgccg atggccatca
     3121 ttcttttgct ggttttgatg gttgccgttg tcgcttttat cgtcctggtc gagcaggggc
     3181 aacgccgtat tccggtccag tacgccaagc gtgttgtcgg ccgccgcgtc atgggtggtc
     3241 agtccaccta tttgccgttg cgcgtcaatt ccggcggcgt gattccgccg atctttgcca
     3301 gctccttgct ggcgtttccg gccacactcg gcctgctcct cggcagccgc tgggttttcg
     3361 tcaaggattt tgcggacaag gtcaaatggg gcgagccgct gtacaccttg ctctatgtca
     3421 cgctgattct gctcttcgcg tttttctatg tcggcatcgt attcaatccg accgaactcg
     3481 ccgacaatat gcgcaagaac ggcggtttca ttcccggcat tcgccctggg cgcaacacca
     3541 gtgaatacgt cagccgcatt ctgaagcgcc ttacgctcat cggtgcggtt tatctggcgc
     3601 tggtttgctt gctgccggaa tggatgatcg ccggcattca cctcaaccac ttgcccacct
     3661 ggctcggtgg cagctggttt gaccgtcact tcccgcagtg gattctgaac ggtctgggcg
     3721 tcaactttta ttttggtggt acatctctgc tgatcgtcgt cggcgtcgct atggacaccg
     3781 tccagcagct ggaagcgcag ttggtcatgc gcaactatga aggattcgca cggaaaggcc
     3841 ggatccgcgg ccggcgctag agtccggtag tcagagggag acgacggagt gacctccgac
     3901 attcggccgc acgagaagct ggaccgtgca gtgatttttc tcggccctcc gggggcggga
     3961 aaagggaccc aggcgaaaga gctggcgcgc cggttttccg cgccgcacct ttccacgggc
     4021 gacatgctcc gcgagcacgt ggcgcagggc acttcgctcg ggcagcgcgc tcagccgatt
     4081 atgagccgcg gcgagctggt tcccgattcg attgtgttac gcatggtcgc cgagcgcatc
     4141 gaacgtccgg actgtacgca tggcttcgtg ttcgatggct ttccgcggac cgtggctcag
     4201 gcgcaatggc ttggcgaact gctcaagcag catggcttca aacagccgct ggttgtgcac
     4261 atggtgatcg acccggcctt gttgttgcgg cgcattaccg gccggcggat gtgcaaagta
     4321 ggcggagaaa tctacaacgt ctacgagcgc ccgcctaaag tggaaggccg ttgcgacaac
     4381 gatggcggcg aactggagca gcgcgccgat gaccgcgaag aaattgtcgg tccgcggctg
     4441 gcagcgtacg agaagcaaac cgctccgctg gcggcgtatt accggcggtt gggacgtctg
     4501 cgcgacatcg acgcctccaa aagcgtggac gatgtcgcgg cgagcatcgt tgcagtcgta
     4561 tgcgacgcac ccaagcagta gtggccgctt gtaagggtcg tttgtaaagg aaatggcgat
     4621 acatctgcgc agtgctgacg aaattgaaaa gatgcaccgt gcgggtctga ttgtgcatga
     4681 agtgctgaac acgctgcgtt ccagcgtgcg tccgggaatg acgacgatgg acctcgagaa
     4741 gatcgccgag gaaaaaatcg ccggcaaacc gggacacccg gctttcaaag ggtatcgcgg
     4801 gtatccctgc gtgctctgca catcggtgaa cagcgagatc gttcacggga tcccttcgcc
     4861 gaagcgcaag ttgaaggaag gcgacatcgt ctccatcgac ttcggcatgg aagtggatgg
     4921 atatttcgcg gactcggcag tcaccgttcc ggtcggcaag atcaaaccgg aattgcagaa
     4981 actgctggat gtgacccgcg actctctcga ccgcgccatc gaacagatgc gcgccggcaa
     5041 ccgcctaggc gatgtaggcc acgcggtgca aagtttggtc gaggagaatg gattttcggt
     5101 agtacgcgaa ttcgtcgggc atggcattgg caccaagatg cacgacgaac cgaacctgcc
     5161 gaattacggg gaacccgggc gcggtgcgcg ccttcaggaa ggtatggtca tcgccgtcga
     5221 accgatggtg aatgccggcc gtcatgaagt gcgcatgcgg gacgaatggg tagccgaaac
     5281 cgccgatggc agtccttcgg cgcattttga gcacaccgta gccgtcacct ccaacggtcc
     5341 gctgattctg acgcggccta aggaaatgac cggcccgtcc tggtagtcgt cagggtgttg
     5401 tagcggccac ctttaggtgg cctccgcggg tttcgtagtg ccgggcttca gccggcatcg
     5461 gtgttacggc ggtaaggctg tgattcagga gcttatggcg aagcacgaag acgacaagaa
     5521 aaaaggcgac tcgggcaatc ccaaagagga cgccattgaa gtcatggcga ccgtgatcga
     5581 gccgctgccg aacgcgatgt tccgcgtcga gctggaaaac aagcaccagg ttctggcgca
     5641 cgtttccggc aagatgcgca agaacttcat ccgcattctg gctggtgaca aagtcgctgt
     5701 tgagctttcg ccgtacgatt tgacccgcgg ccgtatcgtg taccgctaca aatagcggcg
     5761 gcttgggtag cacagaccgg tctgtgcatg ttttgttagg tgctcggtgg ttgcagcggc
     5821 cacattcagg tggcctgggc aaaaaaggga gtcaggcaat gaaagttcgc gcgtcggtaa
     5881 aaaagatttg tgacaaatgc aaggtgatcc atcgccgcgg cgtggttcgg gtaatttgcg
     5941 tcaacccgaa gcacaagcag cgccagggat agtcttgtag cggccgcctt ctgaggcggg
     6001 cgatggtggt tgcggttcga ttttct
//
LOCUS       DASTPM010000226         8681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1218528, whole genome shotgun sequence.
ACCESSION   DASTPM010000226 DASTPM010000000
VERSION     DASTPM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218528"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..924
                     /locus_tag="VFR42_04015"
     CDS             118..924
                     /locus_tag="VFR42_04015"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0368348.1"
                     /translation="MRENRKPLLTITAALAAISLLAGCKSNEQSAQKPSAEPQKTEAP
                     AQTAASAEPTSPNAAVPDREPLHAGKPEAKPTSAAAGTKALLAPKKLTEKAPDAYKVK
                     FDTTRGTFTVTVTRAWSPLGADRFYNLVKHHFYDNAAFFRVVPGFVVQFGISPTPAVS
                     AAWKHTDIKDDPVTQTNKRGSLTFATAGPNTRTTQIFINLKDNARLDGMGFSPFGTVD
                     GNGMNVVEMMYEGYGDNAAPDQDQLEKQGDPYLKKGWPKLDYIKSATLVP"
     gene            1066..2577
                     /locus_tag="VFR42_04020"
     CDS             1066..2577
                     /locus_tag="VFR42_04020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0368349.1"
                     /translation="MPIAPSVYFLAAAAVLIHFLFNRQYGYFRDELYYAACGEHLAWG
                     YVDHAPLVAFASAFTRALFGDSLFALRLLPALSAAPKVFLAGWMARELDGGKFSQFFA
                     ALLVLLAPIYLTFDNFLSMNAFEPVFWMLCAAIVLKIMNGGSTRLWLLFGLVAGIGIL
                     NKHSMLFFGSGLAVGLLLTPARQQFARIEIWLGALIAFLIFLPNLLWEIHNGYPTIAL
                     LHTVIGSKYTTVSPLSFIGEQLLLVNPLAAPIWLSGLWFLLADKSGRKYAVLGIAYLV
                     VLVEMLVLHGKIYYLAPAYMMLLAAGAVAWQRQVFQRAAAWLKPAIIALLVISGIVAA
                     PLAMPILPVAAAVKYTQFFGVQDVKVENVPLNSLPQLFGDMFGWPEQVQAMSRAFHSL
                     SPEEQRRATLLAYNYGEAGAIDYLGKHYGLPKAISGHNQYGYWGPRGATAEIVIAIGF
                     PKSRLEQSFSDVQPFETISTAYALPEESGLTIYLCRRPRQPLSSSWPQWMYLD"
     gene            2969..4678
                     /locus_tag="VFR42_04025"
     CDS             2969..4678
                     /locus_tag="VFR42_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368350.1"
                     /translation="MPEAAAPKETKAQRVERLKREKLAWDHLDEIREFARNGRNSVPP
                     EWLGTYFRSWGIYTQGDGAGVVGGANGEGKSTEFFMVRIRIPNGLLRSEQVRVLADLA
                     ENHARGVADITVRQNLQLHWVTIEAIPDIIEKLQQAGLTTTGACGDVVRNVTGCPVAG
                     LAHDEHFDASPWALAVDRALGGNRDFYNLPRKFKMTVTGCRTWCSYPEINDLAFIATR
                     RVRGSNNEHGFSVRIGGGLSTEPHLALRLNAFVRQDQVVDVARGVAEIFRSRDVLRIS
                     RENARLKYLFLKFDWTAEKFLAELRRVIGYSLDPAEPEDIPSDTHRDHTGIHRQKQAG
                     LAFIGASVLRGRITPSQLRHAAELADQFGDGSLRFTVQQNLLLANVREEHAETVAAKL
                     ASAGLPVEVPAFARGTVACTGSEFCKLALTETKGFARWLSTELDDRLPGFDQQIKLHI
                     TGCPNSCGQHWIADIGIEGKKIKSNGKLVDAYYFCVGGSLGQIASIARPVGYRCAATD
                     VPDAIERLVANFQENRYEKEPLRAFLARKSNDDIREILAGETFAPVERDLPAARTPQS
                     VEG"
     gene            4682..5305
                     /locus_tag="VFR42_04030"
     CDS             4682..5305
                     /locus_tag="VFR42_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HEU0368351.1"
                     /translation="MNLFPMFVKLHGRQVVVVGGGEIAAGKIDGLLKAGASVRLIAPQ
                     VHLSLVSLIRARKVAWIPREFAAGDLEGAALAIAATSAPGVNASVFREAESRGIFCNA
                     VDDIENCHFYYGSVVQRGDLQIAISTSGKSPALAQLLRQELEQQFGPEYEVWLEWLGA
                     ARELLRAADGGSESTKQMLHHLASKTMYEQFVKEAGKKSAASRQGVS"
     gene            5302..6093
                     /gene="cobA"
                     /locus_tag="VFR42_04035"
     CDS             5302..6093
                     /gene="cobA"
                     /locus_tag="VFR42_04035"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEU0368352.1"
                     /translation="MNHIASGKVYLVGAGPGDPDLLTLKAAKLLTEAEVVLYDSLVSR
                     DVLALISQTALLVDVGKRAGKKLLTQDEINSLLVSYAQQGKIVVRLKGGDPLLFGRAG
                     EEMEALRRAAIDFEIVPGITAAVGAAVAAKISLTDRRVASQVLFTTFSRGETSNWLNW
                     AAITPETTIAIYMPGTHYGEVAARLIENGLAPETPCVVVSHATRAEQHLRWSSIAALS
                     DADQLPAPSLLLVGKVATHDVQYCGERVPPDLFSFTDSKLERREQ"
     gene            6090..6221
                     /locus_tag="VFR42_04040"
     CDS             6090..6221
                     /locus_tag="VFR42_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368353.1"
                     /translation="MSQAQNVAFPAKPRRFSLDTWAVLLAFTLAALVRAGVFKHIPW"
     gene            6294..7379
                     /locus_tag="VFR42_04045"
     CDS             6294..7379
                     /locus_tag="VFR42_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEU0368354.1"
                     /translation="MASSLAVAQVRPVNPALQFLKLVPGVLLLAGVGYAGKLLEANVG
                     KYAKAHHWTFPNIEYVLWAILLGLLISNTAGVPEIFRAGVATYEFWLKAGIVLLGARF
                     LLGDVLKLGGTSLALVAIEIVGAIAFMTFLGRVFKLKPKLTSLLAVGSAVCGVSAIIA
                     TQGAIDADEEDSSFAIAAILALGALSLFTFPLIGHSLHLTDHAYGVWSGLAVDNTAEA
                     AAAGALYSDAAGKLAVLTKTTRNALIGFVVLAYAFYWASRGQAQAVGNKAKFLWQKFP
                     KFVLGFLLISTLATFAFFSKEQIGALANLSRWAFLLTFAGVGLRTNFRTLSKQGLKPF
                     LVGAIGEIAIAGFTLGLVLAAQRIFGW"
     gene            7490..8116
                     /locus_tag="VFR42_04050"
     CDS             7490..8116
                     /locus_tag="VFR42_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368355.1"
                     /translation="MPVRIRRVTAILVLASSLAFAANDKKPVLPAYVLRAETVFVLID
                     PDAGTSPASPQANQKARDDVEKAIMKWGRFRLVMEPGSADLIVVVRKGSGKVVQPTIG
                     GLPTNDRPVIGQPTDNGIRLGGQKGRPPGSTDPSPQDTHPTPQIEAGMTDDMFVVYQG
                     GAGNPLDGPAAWRYMNKDALKSPSVPAVAEFRKAIEEAEKQQQQKKKP"
     gene            8195..>8681
                     /locus_tag="VFR42_04055"
     CDS             8195..>8681
                     /locus_tag="VFR42_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0368356.1"
                     /translation="MQNPTRRSFLIASGLTALSATRILGANDTLRIGVIGAGGRMNDL
                     LNAADKAGGVQITAVSDVYAPHLDAVKQRAGAKDATAHADYRELLQQNVDVVLIASPD
                     HWHVRMAVDALNAGKDVYLEKPVTHTIEEGGALVKVVRSNKKILQCGMQQRSWTHFRD
                     AV"
BASE COUNT         1771 a         2829 c         2305 g         1776 t
ORIGIN      
        1 ggcgtcgagc gagtgagcag ggaactgaac gccaagcagt aactcattgt ctggatccga
       61 tttcgctcgg cgagcggcgt ccggacaaca cgaaaactgt ttaggagaga agcgagcatg
      121 cgggaaaatc gaaagccctt acttacaatt acggcggctc tagcagccat ttcgctcctt
      181 gccggctgca agagcaacga acagtcggcc caaaagcctt cggccgagcc gcaaaaaacc
      241 gaagcgcccg cgcaaaccgc cgcgtccgcg gaacctactt ccccgaacgc cgctgtaccc
      301 gaccgcgaac cgctccacgc gggaaaacct gaagccaaac caacgagcgc cgctgcaggc
      361 acgaaagctc ttctcgcgcc caaaaaactc accgaaaaag cccccgacgc ctacaaagta
      421 aaattcgaca ccacccgcgg caccttcacg gtgaccgtta ctcgtgcctg gtctccgctc
      481 ggtgccgacc gcttctacaa tctggtcaaa caccattttt atgacaacgc tgccttcttc
      541 cgtgtcgtcc ccggcttcgt cgtgcaattc ggcatcagtc ccacgcccgc cgtttccgcc
      601 gcatggaagc acaccgatat caaggacgat cccgtcacgc aaaccaacaa gcgcggctcc
      661 ctcaccttcg ccaccgctgg cccgaacaca cgcaccacac agattttcat caatctgaaa
      721 gataacgctc gcctcgacgg catgggcttc tccccattcg gcaccgtcga cggcaacggc
      781 atgaacgtcg tcgaaatgat gtatgaaggt tacggcgaca acgccgctcc cgatcaggat
      841 cagctcgaga agcagggtga cccgtacctc aaaaagggtt ggccgaaact cgactacatc
      901 aaatccgcca ctctggttcc gtagagcggt tcgtcctcaa gacgatccgc tctctggctc
      961 aagtacgtgt ccgcaccagt caatacttcc gttattccga gcgaagcgag gaatctctct
     1021 tcggacgcag ccacctcgct tcgcgggaac aaaactgaaa cacttttgcc cattgcgccc
     1081 tccgtttact tcctcgccgc ggcagccgtc ctcatccact tcctcttcaa ccgccaatac
     1141 gggtacttcc gcgatgaact ctactacgcc gcctgcggcg aacacctcgc ctggggctat
     1201 gtcgaccacg cccctctcgt agccttcgct tccgctttca cccgagcgct cttcggcgac
     1261 tcgctcttcg ctcttcgcct tctgcccgct ctctccgctg ctcccaaagt ttttctcgcc
     1321 ggatggatgg ctcgcgagct cgacggtggc aaattctcac aatttttcgc agcactgctc
     1381 gtcctcctcg ccccgatcta tctcaccttt gataacttcc tctccatgaa cgccttcgaa
     1441 ccggttttct ggatgctctg cgccgccatc gttctgaaaa taatgaacgg cggcagcacc
     1501 cgcctctggc ttctgttcgg actcgtggct ggcatcggca tcctcaacaa gcactccatg
     1561 ctcttcttcg gttcgggact cgccgtcgga ctactcctta ctcccgcccg ccagcagttt
     1621 gcgcgcatcg aaatctggct cggtgcgttg atcgctttcc ttatattcct tcccaatctg
     1681 ctctgggaaa ttcacaacgg ctatcccacg atcgctctct tgcacaccgt gattggcagc
     1741 aagtacacaa cggtctcgcc gctctccttc atcggcgaac aactcctcct ggtgaaccct
     1801 ctcgccgcgc ccatctggct ttccggctta tggtttttgc tggctgacaa gtccggccgc
     1861 aaatacgccg tcctcggcat tgcttatctc gtcgtcctcg tcgaaatgct ggtgctccac
     1921 ggcaaaatct actatctcgc ccccgcctac atgatgctgc tcgccgcagg cgcggtcgct
     1981 tggcaacgcc aggtatttca gcgcgcggcg gcttggctga aacccgcaat cattgccctg
     2041 cttgtcatat ccggaatcgt cgctgctccc ctcgccatgc ccatcctccc ggttgccgcc
     2101 gccgtgaagt acacccaatt cttcggggtt caagacgtca aagtggaaaa cgtgccgctg
     2161 aactcgcttc cgcaactctt cggcgatatg ttcggctggc ccgaacaagt ccaggccatg
     2221 tcccgcgcat tccattccct ttcgcccgaa gaacaacgcc gcgcaaccct actcgcctac
     2281 aactacggcg aagccggcgc catcgactac ctcggcaaac actacggctt gccgaaagcc
     2341 atcagcggcc ataaccagta cggctattgg ggcccgcgcg gcgccaccgc cgaaatcgtc
     2401 atcgccatcg gcttccccaa gtcccgcctc gagcaatcct tctctgacgt ccaacctttc
     2461 gaaaccatca gcaccgctta cgccctcccc gaagaatccg gcttaaccat ctacctctgc
     2521 cgccgacccc gccagcccct ctcctcctcc tggccccaat ggatgtacct cgactaacta
     2581 aggcttttgg attgggacag cgtgccgcgc agggatccac tgtttttcca atttcccttt
     2641 tctattttcc accgcataac cactccaccc gccccagcat ccaaattatc gttcgcaagc
     2701 atcatctttt tgcgagcaca aacggcgttg acacggcccg ctacgccgcg tactctgcta
     2761 acgtaatgcg aatgacctgc cagaccgtgt gttgttgctg ctgcgactgc tgttgctgtc
     2821 gcgaagcaca catttctggc ttgaggcggc tgcggaccta gaaccgcact aaacaaagaa
     2881 attccaaagc ccacgtgcca gaaatactgg tccacgtggg ctttttcttt ttctaaacaa
     2941 actctctgca attaaggaac aacggcaaat gccagaagca gcagcgccaa aagaaacgaa
     3001 agcccagcgc gtcgaacgcc tgaaacgcga aaagctcgcg tgggaccacc tcgacgaaat
     3061 ccgcgaattt gcgcgcaacg gccgtaattc cgtcccaccc gaatggctcg gcacctattt
     3121 ccgttcctgg ggcatataca cacagggcga cggcgccggc gttgtgggtg gcgcgaacgg
     3181 cgaaggcaag tccaccgaat ttttcatggt ccgcatccgc atccccaacg gtcttttgcg
     3241 tagcgagcag gttcgcgtac tcgccgatct cgcggaaaat cacgcccgcg gcgtcgccga
     3301 catcaccgtc cgccaaaatc tccaactcca ctgggtcacc atcgaagcca ttcccgacat
     3361 tatcgaaaag ttgcagcagg ccggcttgac caccaccggt gcatgcggcg acgttgttcg
     3421 caacgttacc ggctgccctg tcgctggcct tgctcacgac gaacatttcg atgcatcccc
     3481 ctgggccctt gcggtagacc gcgctcttgg cggcaatcgc gacttctaca accttccgcg
     3541 caaattcaaa atgaccgtca ccggctgccg cacctggtgt agctatcctg aaatcaatga
     3601 cctcgccttc atcgccacgc ggcgcgttcg cggttcgaac aacgagcatg gcttttcggt
     3661 tcgcatcggc ggcggcctct ccaccgaacc gcatctcgcg ctccgcctca acgcctttgt
     3721 ccgccaggat caagtcgtcg acgtcgcccg cggcgtcgca gaaatcttcc gctcccgcga
     3781 tgtgcttcgc atcagccggg aaaatgctcg cttgaaatat ctcttcctca aattcgactg
     3841 gactgcggaa aagttcctcg ccgaactgcg gcgcgttatt ggctactccc tcgatcccgc
     3901 cgaaccggaa gacattcctt ccgacacgca ccgcgaccac acgggaatcc atcgccagaa
     3961 acaagccggt ctcgccttca tcggtgcttc cgtgctgcgc ggccgcatca cgccgtccca
     4021 gcttcgccac gccgccgaac tcgccgacca gttcggtgac ggcagcctgc gcttcacggt
     4081 tcaacaaaac ttgctgcttg ccaatgtccg cgaagagcac gccgaaaccg tcgccgctaa
     4141 actcgcaagc gctggccttc cggtggaagt tccggcattc gcgcgcggca cggtcgcctg
     4201 cactggctcc gaattctgca agctggccct caccgaaacc aaaggcttcg cccgctggct
     4261 ctccaccgaa cttgacgacc ggctgcccgg cttcgaccaa cagatcaagc tgcacattac
     4321 cggctgcccc aacagttgcg gccagcactg gatcgctgac atcggcattg aaggcaaaaa
     4381 aattaaatcc aacgggaagt tggtggacgc ctactacttc tgcgtcggcg gcagtctcgg
     4441 gcaaatcgct tccatcgcgc ggcctgtcgg ctatcgctgt gccgccaccg atgttcccga
     4501 tgccatcgaa cgcctggtcg caaattttca ggaaaatcgt tacgaaaaag aaccgcttcg
     4561 cgctttcctt gcgcgcaaat ccaatgacga tatccgcgaa atcctcgccg gcgaaacgtt
     4621 tgcgccggtc gagcgcgatc tgcccgcggc gcgtacaccg caaagtgtgg agggctgacc
     4681 gatgaacctt tttcccatgt tcgtgaaatt gcacggacga caggtcgtcg tcgtgggcgg
     4741 cggtgagatc gctgccggca agatcgacgg cctcctcaaa gccggagcaa gcgttcgcct
     4801 gatagctccg caagttcact tgtctttagt aagtctgatc cgggcgcgca aggttgcatg
     4861 gattccgcgt gaattcgcgg caggcgatct ggagggcgcg gcactcgcga tcgccgccac
     4921 ttcagcgccc ggcgtcaacg ccagcgtctt tcgcgaagcc gaatcgcgcg gcattttctg
     4981 caatgccgtg gacgacatcg aaaactgcca cttctactac ggctcggtgg tccagcgcgg
     5041 cgacttgcaa atcgccatct ccaccagcgg aaagagcccc gcgctcgcgc agcttctgcg
     5101 tcaggaactc gaacagcaat tcggccctga gtacgaagtg tggctcgagt ggctgggcgc
     5161 ggcgcgtgaa cttcttcgtg cagccgatgg cggtagcgaa agcaccaagc agatgctgca
     5221 tcacttggcc agcaagacga tgtacgaaca gtttgtaaaa gaagccggca agaagtcagc
     5281 ggcatcgcgg caaggtgtct cgtgaatcac atcgccagcg gaaaagtcta tctggtcggc
     5341 gctggcccgg gcgatcccga tctgctcacg ctgaaagccg cgaagctcct cacggaagct
     5401 gaggtcgtcc tgtacgactc gctggtgagc agggacgtac tcgctctgat ttcccaaact
     5461 gctcttctgg tagatgtcgg caagcgtgcc ggaaaaaagc tgctcacaca ggatgaaatc
     5521 aacagcctgc tggtgagcta cgcgcagcaa ggaaaaatcg tcgtgagatt gaaaggcggc
     5581 gatccgctgc tctttggccg cgccggcgaa gagatggaag ccctgcgccg cgccgcaatc
     5641 gattttgaaa tcgtgccggg aatcactgca gctgtcggcg ccgcagtagc cgccaaaatc
     5701 tctctcactg atcgccgcgt agcctcgcag gttctcttca caacattttc ccgcggcgaa
     5761 actagcaatt ggctgaactg ggcggccatc actccggaga ctacgattgc gatttacatg
     5821 cccggcacgc actacggcga agttgccgcg cgcctcatcg agaacggcct ggcgccagaa
     5881 acgccatgcg tggtggtgtc gcacgcaaca cgcgccgagc aacacttgcg ctggtcctcc
     5941 attgcggcgc tttcggacgc cgaccaattg cctgcaccgt cgctgcttct cgttggcaaa
     6001 gttgccacgc acgatgtgca gtattgtggc gaacgcgttc cgcccgacct tttttcattc
     6061 acagattcaa aactggagag gagagagcaa tgagtcaggc acaaaatgtg gcgtttccag
     6121 cgaagcccag acggttttcg ctggacacct gggcggtgct gctggcgttc acgctggccg
     6181 cgttggtccg cgccggcgtc ttcaaacaca tcccctggtg agtcttgtag tggccgacca
     6241 ggaagtcgct tttcttgagg gcgttgtttc atttgacgct gaagaggtga ggcatggcaa
     6301 gttcgctagc agttgcgcag gtcaggccgg tgaatccggc gctgcaattt ttgaagctgg
     6361 tgcccggagt gctgctcctg gcgggcgtgg gctatgctgg caaactgctg gaagccaacg
     6421 tcggcaaata cgccaaagcg catcactgga catttcccaa tatcgagtac gtgctgtggg
     6481 cgattctgct gggcctgctg atctcgaata cggcgggagt tccagaaatt tttcgcgccg
     6541 gcgtggcgac gtatgagttc tggcttaaag ccggcatcgt cctgctcggc gcgcgattct
     6601 tgctcggcga cgtgctgaaa ctcggcggca ccagcctggc gctggtggcg attgagattg
     6661 tcggcgcgat tgccttcatg acatttctcg gccgcgtatt caagctgaag ccgaagctca
     6721 cctccttact cgccgttggc tcggcggttt gcggcgtcag cgccatcatc gccacgcaag
     6781 gagccatcga cgctgacgaa gaagattcct cgttcgctat cgctgcgatt ctcgcgctcg
     6841 gcgctctttc tcttttcacg tttcctctca tcggccactc gttgcacctc accgatcacg
     6901 cctacggcgt ctggtccgga ctcgcggtgg ataacactgc cgaagccgcg gctgccggcg
     6961 ccctgtactc cgacgccgcc ggaaagctgg ctgtactcac caagaccacg cgcaacgcgc
     7021 tgatcggttt tgtcgtcctg gcctatgcgt tctattgggc cagccgcggg caagcgcaag
     7081 ccgttggcaa caaagcgaag ttcctgtggc agaaatttcc caagttcgtg ttgggcttcc
     7141 tcctgatctc tacgctggcg acgttcgcat ttttttccaa ggaacaaatc ggcgcgctgg
     7201 ccaatctttc acgctgggcc tttctgctaa catttgccgg ggtcggcctc cgcaccaatt
     7261 tccgcactct ttccaagcag ggactcaagc cttttctcgt tggcgccatc ggagaaatcg
     7321 ccatcgccgg tttcacgctt ggcttggtgt tggcggccca gcgcatcttc ggttggtgat
     7381 ggacagcttc gcgacggcaa gtcaagccgg tttgtcgctg gaccagtcga caccgcgcca
     7441 gcgaaggccc gccggcgtat gatgaacttg cccgctctgg gaggttctga tgcccgttcg
     7501 tattcgccgg gtaactgcaa ttttggtttt ggcttcgtcg ctggctttcg cggcaaatga
     7561 caaaaagccc gtcctgccgg catacgttct gcgcgccgaa accgtgtttg tattgatcga
     7621 tccagacgcc ggaacgtccc cggcctcgcc gcaggcgaat caaaaagctc gtgatgatgt
     7681 cgagaaggca ataatgaaat gggggcgctt tcgcctggtg atggaaccgg gcagcgccga
     7741 cttgatcgtc gttgtgcgca agggctcggg caaagtggtc cagccgacca tcggcgggtt
     7801 gccaacgaat gaccggccgg tgattgggca gccaaccgat aacggcatcc ggctcggggg
     7861 gcaaaaagga cgtcctcccg gctccacgga tccatccccg caggacacgc atccgacacc
     7921 tcaaattgag gcgggcatga ccgacgacat gttcgtggtc taccagggag gtgcaggcaa
     7981 tccattggat ggtccagcgg cctggcgcta catgaataag gatgcgctga agtcgccgag
     8041 cgttcctgcc gttgcggaat tccgcaaggc catcgaagaa gcagaaaaac aacagcagca
     8101 gaaaaagaaa ccttgagcgc agaaccccgc ggctgagttt gaaacctaaa acagcgcctt
     8161 tccggctcgc gcaggtacgg ctaagatagg caccatgcaa aatccgactc gtcgtagctt
     8221 cctcatcgct tccggactca ccgcactttc cgctacccgc attctgggcg ccaacgacac
     8281 gctgcgcatc ggcgtcatcg gcgcgggcgg ccgtatgaat gatctgctga acgctgccga
     8341 caaagctggc ggcgttcaga tcaccgcggt cagcgacgta tacgcgccac accttgacgc
     8401 cgtcaagcaa cgcgcaggcg cgaaggatgc aaccgctcac gcagactatc gcgaactgct
     8461 ccaacaaaac gtcgatgttg tcttaattgc ctcaccggac cactggcatg tgcgcatggc
     8521 agtggacgcg ctgaatgccg gcaaggatgt ctacctcgaa aagccggtaa cacacaccat
     8581 tgaggaaggc ggggcgctcg tcaaagtggt gcgttcgaac aaaaagattc tgcaatgcgg
     8641 catgcagcaa cgcagctgga cgcacttccg cgatgccgtg g
//
LOCUS       DASTPM010000227         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_123323, whole genome shotgun sequence.
ACCESSION   DASTPM010000227 DASTPM010000000
VERSION     DASTPM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123323"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFR42_04060"
     CDS             <1..735
                     /locus_tag="VFR42_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0368357.1"
                     /translation="EQPILKDLSFKIKAGQVAAFVGPTGAGKTTIISLVARFYDPTNG
                     VIKIDGRDIRTYKLRSLRGQMSFVLQETLLFRAPVWQNIAYGKPQASRAEIIKAAEIA
                     NAAEFIDKLPNGYDTLLGERGVTLSGGQRQRIAIARAVLRDAPILILDEPTSSLDASA
                     ETLVVEALDRLMKGKTCIVIAHHLSTIRNADVIFVVKDGAIAEYGTHSELLVAGGIYT
                     ELFNAQSQPLNGEGADIPKDVEPVER"
     gene            complement(748..1905)
                     /locus_tag="VFR42_04065"
     CDS             complement(748..1905)
                     /locus_tag="VFR42_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368358.1"
                     /translation="MTGLRWYKLLLLGMVSVAALPYPSAHPAQAAFAADERKPAPDPV
                     KPVSISALWHDPGEISSLDLAFGEGGPAHAPPGDAIYSFEKEDLGGSSPKFYVKDQNG
                     VQWLVKVGDEARGETAATRFVWAMGYFVDEDYFVPEIHVSGIAKLHRQSHSIHKDGTI
                     TNARLKRTPPKQKKLNTWAWADNPFRGSRELNGLRVMMALINNWDLKTVNNKVYGRKE
                     GKDAAEKGDSDGKEENSTDVADGDHNSSPQIPEGERNVANVRYVVSDLGASFGRTGGF
                     SSRSKGKLKDYQKDNFIRATTPETVDFVMRTKPGGILRVFKPHYYRQRTAMAETVEHV
                     PRADARWIGRQLAQLTPEQIRSAFLAAGFTPREADGYLVELQKRIVELNAL"
     gene            2094..>2430
                     /locus_tag="VFR42_04070"
     CDS             2094..>2430
                     /locus_tag="VFR42_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU0368359.1"
                     /translation="MKVLVQPQDGAHPILSAITRAKKMVEIVIFRLDRNQIEEAVRAA
                     AARGVFVRALVAFANRGGEQQLRRLETRFLDSGVTVTRTSGDLVRYHAKMMIIDRKTL
                     YVLSFNYTTL"
BASE COUNT          493 a          635 c          781 g          521 t
ORIGIN      
        1 gagcagccga ttttgaagga cttgagcttc aagattaaag cgggacaagt ggcagcgttt
       61 gtgggaccga ccggcgcggg gaagacgacg atcataagcc tggtggcgcg gttttatgat
      121 cccacgaatg gcgtgatcaa aatcgacggg cgggatatcc ggacatacaa gttgcggtcg
      181 ttgcgcgggc agatgagctt cgtgctgcag gagactctgc tgtttcgcgc gccggtctgg
      241 cagaacatcg cgtacggcaa gccgcaagcc tcgcgtgcgg agatcatcaa ggcggcggag
      301 atcgcgaacg cggcggagtt catcgacaag ttgccgaacg gctacgacac gctgctggga
      361 gagcgcggcg taacattgtc gggcgggcaa cggcagcgca tcgccattgc gcgagcggtt
      421 ttgcgcgatg cgccgatatt gattctggat gagccgacgt cgagcctgga cgcttccgcg
      481 gaaacgctgg tcgtggaagc gctggaccgg ctgatgaaag gcaagacgtg cattgtgatc
      541 gcgcatcatt tatcgaccat cagaaacgct gacgtgatct ttgtggttaa ggatggagcg
      601 attgcggaat acggcacgca cagcgaactg ctggtagccg gcggaattta caccgagctt
      661 ttcaatgcgc agtcgcagcc actgaacggc gagggagcgg acataccgaa ggatgtcgag
      721 ccggtggagc gctgacccgc tgcagcctca cagcgcgttc aactcgacaa tgcgcttctg
      781 cagttcgacc aggtagccgt ccgcctctct tggcgtgaag ccggcagcca ggaaggcgga
      841 gcgaatttgc tccggagtga gttgtgcgag ctgtcggccg atccatctgg catcggcgcg
      901 gggaacatgt tctacggtct cggccatggc ggtgcgctgt cggtagtagt gcggtttgaa
      961 gacacgcaga attccgcctg gtttggtgcg catcacaaag tcgaccgttt cgggcgtggt
     1021 ggcgcgaata aaattatctt tctggtagtc cttgagcttc ccttttgaac ggctggagaa
     1081 gccacccgtg cggccgaagc tggcgccgag gtcgctgaca acgtagcgga catttgcaac
     1141 atttctttcg ccctcgggaa tttgtggcga gctgttgtgg tccccgtccg cgacgtccgt
     1201 ggaattttcc tctttgccgt ctgaatcacc cttctcggcc gcgtctttgc cttccttcct
     1261 gccgtagact ttgttgttta cggttttgag atcccagttg ttgatcaggg ccatcatgac
     1321 gcgaaggccg ttcaattccc tgctgccacg gaagggattg tcagcccagg cccaggtgtt
     1381 gagctttttc tgtttgggtg gagtacgctt gagcctcgcg ttggtgatgg tgccgtcttt
     1441 gtggatcgag tggctctggc gatgcagctt agctatgccg gagacatgga tttcaggcac
     1501 aaagtagtcc tcgtcgacaa agtaacccat ggcccagaca aagcgtgtag cggcggtttc
     1561 gccgcgagct tcgtcgccga cttttacgag ccattgcacg ccattctgat ctttgacgta
     1621 gaacttgggg gaagatccgc cgaggtcttc tttttcaaag gagtagatcg catcgccagg
     1681 tggagcgtgc gctgggccac cctcgccgaa ggcaaggtcc agcgaggaaa tttcacccgg
     1741 gtcgtgccaa agagccgaga tggatacagg cttaaccgga tcaggagcag gtttgcgttc
     1801 atctgccgca aatgcagcct gagctggatg agcagaagga tacggaagcg ccgcaacgct
     1861 taccattccg agcaaaagca gcttgtacca gcgaaggccc gtcataatct tgtctccctg
     1921 caaggccggc aggcaaaagt aggcattttg gcgggggttg gcaatcgggg aatctactgt
     1981 gttgaaccga ttgtggcctt acggagacag gccctagcct gcatcaccgg caagaaattg
     2041 ggcagttgct gcgtttgggc caggtaggga ccgacaacgg aggaacttcg cctgtgaagg
     2101 tactggtaca gcctcaagac ggcgcgcatc ccattttgag cgccattacg cgcgcaaaaa
     2161 agatggtaga gattgtgatc ttccgactgg accgcaacca gattgaggag gccgttcggg
     2221 cggcggcagc gcggggtgta tttgttcgcg ccctggtggc ctttgccaat cgcggcggag
     2281 agcagcaact gcggcgcctg gaaacgcggt ttctggattc cggcgtgacg gtgacgcgca
     2341 ccagcggcga cctggtgcgg tatcacgcga agatgatgat catcgacagg aagacgctgt
     2401 acgtcctctc tttcaactac acaacgctgg
//
LOCUS       DASTPM010000228         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_382716, whole genome shotgun sequence.
ACCESSION   DASTPM010000228 DASTPM010000000
VERSION     DASTPM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382716"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1219)
                     /locus_tag="VFR42_04075"
     CDS             complement(314..1219)
                     /locus_tag="VFR42_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0368360.1"
                     /translation="MSSAPKTPRARPTGSPPSVIASRDNKWLKEFRMALRGGLPTESG
                     AVGVEGPRLVEEALRSACPIRAVLFSETGERHRARLAQYLDRPEVSIPLLHTTDRLFE
                     GIADTEHPQGVAALAAPRTFTFEELLRSPSNSGEKCGSALILVLVGVQDPGNVGTIIR
                     TAAAFGASAAITAASGQSGTASPFNPKALRASAGAALHLPVLAGQTAAAAVEQLRGAG
                     IHVLASSSRESADFDQPLLTPWQVNWCQPVALLIGNEGQGLPEEIERSALARIRIPMS
                     SSVESLNAAAAAAVLFYEAARQRSG"
     gene            1449..>2474
                     /locus_tag="VFR42_04080"
     CDS             1449..>2474
                     /locus_tag="VFR42_04080"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEU0368361.1"
                     /translation="MKTKILCGLAVTAALAWFGAGEANAQVKLIAKGTLTESSAGLFA
                     DLSGLDYNLENGAPANLLGGLGSGIAWGFGDTFLALPDRGPNAVSFDSLVDDTVSYVP
                     RFHTIKMSLQPSSGSLPFTLTPTLEATTLLYNASPLFYGSGSGLGVGPGAPVINTPSR
                     NYFSGRSDNFDPSGNSGNANNARLDCEGIRLSSDGKRVYISDEYGPYIYEFDRSSGQR
                     IRAFKLPDHLFVSTLSAQGALEIANNTIGRVANKGMEGLAITPDGNTLVGIMQNALIQ
                     DAAQGGKAANLLRIVTVNTSTGKTTHEYAYLLTTGSGVSEITALNDHEFLVDERDGKG
                     LGDGSKAK"
BASE COUNT          533 a          709 c          767 g          465 t
ORIGIN      
        1 atcaaggaag gcgatgagtt caactacagc atcatgaata tgcagagtat gaatgccttg
       61 accggccaag gcggcggccc gggcaaaaac tgattctgct ttcagaaagc cgcccactac
      121 caggatttcc tgtcgggacg catctgccca tggcagttcc gtactggtgt gcccgtaagg
      181 acacccgtcc tgctgcacct tctggcgacg aagaggatcc ttacccggac gagccctgct
      241 acgattcaag aattagttcg tcgcgcggcc gatcgcgttg atttccgtgt tggtggggag
      301 ttagtgtccc ggcttaacca gatcgctggc gagcagcttc gtagaacaat accgccgcgg
      361 cagcggccgc attcagcgat tcgacagaag aggacatggg gatgcgaatt ctcgcaaggg
      421 cgctgcgctc gatttcttct ggcagtcctt ggccttcgtt gccgatcaac agcgctactg
      481 gctggcacca gttcacctgc cacggagtca gcagcggctg atcgaaatcc gctgactcgc
      541 gcgaactgga cgccagcaca tgaatgcctg cgccgcgcaa ttgctcgacg gctgctgctg
      601 cagtttgtcc cgcgagcacc ggcaggtgca aagcggctcc cgccgatgcg cgcagagcct
      661 tgggattaaa aggactcgcc gtcccgcttt gtcccgaagc ggcggtgatc gcggcggagg
      721 cgccaaaggc tgcggcagta cgaatgatcg tgccgacgtt gcccgggtcc tgcacgccca
      781 ccagcaccag gatgagcgcc gagccgcact tttcgccgga attggaaggg gaacgtaaaa
      841 gttcctcgaa cgtaaaagta cgaggagccg ccagagccgc cacgccttgc gggtgttcgg
      901 tgtccgcaat gccctcgaag agccggtccg tggtatgaag aagggggatg gaaacttccg
      961 gccgatcgag atattgcgcc agccgcgcgc ggtgccgctc gccggtttcg ctgaaaagca
     1021 cggcgcgaat gggacaagcc gagcgcagcg cttcttccac caggcgcggg ccttccactc
     1081 caacggcgcc ggattctgtt ggcaggccgc cgcgcagcgc catgcgaaac tccttgagcc
     1141 acttgttgtc gcgcgaagcg attaccgatg gcggcgatcc ggtgggacgc gcgcgcggcg
     1201 tttttggggc agaggacatt ccgggtgcga gcgtagcaga gtttttgctg aatcggggaa
     1261 ttcgcaacga gtggacgagg agaaaaccac cactacttcg catcgaagcc gatgcccgaa
     1321 aggttgtgag aatcgcgaaa caatatattc aaccaacgtt aacccaattc ctgaaccccg
     1381 ccgcaacaat cgtcgcgtcc cactgcacgg cgcttcagga gagaacaatc caaagggaga
     1441 aacgtttcgt gaaaactaag attttgtgtg gtttagcggt gacagcggcg ctggcctggt
     1501 ttggcgccgg ggaagcgaac gctcaggtaa aactgattgc gaaaggaacc ttgaccgaat
     1561 cgagcgcagg gctgtttgcc gatctatccg gactggatta caacctggaa aacggggcac
     1621 cagcaaattt gctgggcggc ctgggttcag gaattgcctg gggctttggc gatacgtttc
     1681 tcgccttgcc cgatcgcggg cccaacgccg tgagtttcga ttcgttggtg gacgacacgg
     1741 tgagttatgt tccgcgcttc cacaccatca agatgagcct gcagccgagc agcgggtcgt
     1801 tgccgttcac gctcacgccg acgctcgagg cgacaacgct gctctacaat gcctcgccgc
     1861 ttttctatgg aagcggatca gggctgggcg tcggacccgg agcgccggtg attaacacgc
     1921 cgtcgcgcaa ttatttttcg ggccggtcgg acaatttcga tccgagcggg aattccggca
     1981 atgccaacaa tgcgcggctg gactgcgaag gaatccggct gtcgagcgac ggcaagcgcg
     2041 tttacatctc cgatgagtac ggcccataca tttacgaatt tgatcgcagc agcgggcaga
     2101 gaatccgggc gtttaagttg ccggatcatc tgttcgtttc cacgctgagc gcgcaaggcg
     2161 cgctggagat tgccaacaac accatcggcc gagtggccaa caagggcatg gaagggctgg
     2221 cgattactcc tgacggcaac acgctggtcg gaatcatgca gaacgcgctg attcaggatg
     2281 cggcgcaagg tgggaaggcc gcgaacttgc tgcgcatcgt gaccgttaac actagcaccg
     2341 gaaaaaccac gcacgagtat gcgtacttgt tgaccaccgg ttccggcgtc agcgaaatca
     2401 ccgcgctgaa cgatcacgaa tttctggtcg acgaacgcga cggcaaagga ctcggcgacg
     2461 gcagcaaggc caag
//
LOCUS       DASTPM010000229         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_584305, whole genome shotgun sequence.
ACCESSION   DASTPM010000229 DASTPM010000000
VERSION     DASTPM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584305"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="VFR42_04085"
     CDS             complement(<1..1781)
                     /locus_tag="VFR42_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0368362.1"
                     /translation="MRFGVYEVDPRAGELRRNGVKIKIQEQPYQVLALLLERPGEVVT
                     REELKRRLWPADTFVDFDHSLNAAIKRLRDVLRDSADNPRFVETLARRGYRFLAPVTG
                     PLAAPVPAISVVPPTYWRSTNRARWIAAIVVLAGACISVGFHIGNRAARAEVPREAKL
                     TANSPDIPVYRAAISPDARLLAYTDPRGLFLREIAREDSHQLWLPPGLKPNVVSWFPD
                     GNHLLVGGVQSEGEQPSLWNVSILGGPSHKISDETDSGVVSPDGTRIAALRGNGKLNA
                     SELWLMNSDGSQPRKLLDIAGYSNSLTWSPSGTRLAYMKASYWPGYKEEVHIETIDLA
                     TGETKTTFSDDKIGGGLVWSRDGRLFFSRAEEDSNIKSDIWSLRVDERTGEARGNIAR
                     LTNSPDWKPRPEVSADGRKMIFLRNNIAPTVYLAQVDARTKNLDQLERLTLDESVSRP
                     YEWTPDGTALLYVSNRDGEFHIFRQRLGAASPELLVDGHDNPNIMRLNPDGTQILYMS
                     EASRAGRSGTTPAEHTEGFDREKVRIMTAPIAGGVGKLVLEADGMNNFQCARAPSTFC
                     LFSRFTKDALAFVQFDSATGEMKDFFQT"
     gene            2058..2714
                     /locus_tag="VFR42_04090"
     CDS             2058..2714
                     /locus_tag="VFR42_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368363.1"
                     /translation="MWRKFQLARAFGWLFISLLPPVVSAAPDAHNMPVSLTVSIFNDA
                     GVPASVLRQAELRARAVLMDAGVTLTWLDCGVPGQWRTKLGCQAIVFPTHFSVRLVAG
                     RQGVSDDTFGQSYLNERGEGNYAYVYVTPLSSARALSVIKEGDLLGYVVAHELGHLLL
                     GKESHSAGGLMRARWEVAELQEGARGKLLFSRGEAEEIRGRYLAAAARAGILVAGAGK
                     "
     gene            complement(2737..>3503)
                     /locus_tag="VFR42_04095"
     CDS             complement(2737..>3503)
                     /locus_tag="VFR42_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU0368364.1"
                     /translation="GMGSTIGDYNGDGRLDIFKTNFSDDTATLYRNNGDGTFDDVTFP
                     AGLGLYTKYLGWGTMFLDVDNDGWPDLLLVNGHVYPEVDSQHLGSDYLEPRILYHNKG
                     NGTFEDISASAGPAITAAASGRGLAIGDLWNDGRISAVISNMNAPVSLLVNQERSSNH
                     WIAFKTVGTKSNRDGIGAKIRVKAGERILVDEVRSGSSYVSNNDMRVHFGLGAISKPE
                     WVEVRWPSGLTETFVELRPDSINTLNEGSGVVKANR"
BASE COUNT          691 a          960 c         1076 g          776 t
ORIGIN      
        1 gtttgaaaga aatctttcat ctcgccggtt gcggaatcaa attgcacaaa ggcaagcgca
       61 tctttcgtaa accggctgaa cagacagaaa gtcgatggcg ctctggcgca ttgaaagttg
      121 ttcatgccgt cggcttcaag aacaagtttg cctacgccgc cggcgatggg cgcggtcatg
      181 atgcgaactt tttcgcggtc aaatccttcg gtgtgctcgg cgggggtggt tccgctgcgt
      241 ccggcgcggc tggcttcact catgtagagg atctgagtac cgtcagggtt gaggcgcatg
      301 atgttcggat tgtcatggcc atccacgagc agctcaggag aagcggctcc cagacgttgc
      361 cggaaaatgt ggaattcgcc gtcgcgattc gagacgtaga gcagcgcagt tccgtccgga
      421 gtccattcat agggccggct gacactttcg tcgagggtca agcgttccag ttgatccagg
      481 ttctttgttc ttgcgtcgac ttgcgcaaga taaacggtgg gagcgatgtt gttgcgcaga
      541 aaaatcattt tccggccgtc ggcggatact tcaggacgtg gcttccaatc cgggctgttg
      601 gtcaggcggg cgatattgcc tcgggcttct ccggtgcgtt cgtcgacacg caacgaccag
      661 atatcggact tgatgttgga atcttcctcg gcacggctga agaagaggcg gccatcgcgg
      721 ctccacacca ggccaccacc gatcttgtcg tcggaaaaag tggtcttcgt ttcgcctgtg
      781 gcgagatcga ttgtttcgat gtggacttct tctttgtagc ccggccagta actggctttc
      841 atgtaagcga ggcgcgtgcc actgggcgac cacgtcagtg agttggagta tccggcgata
      901 tccagaagct tcctgggctg actgccatcc gagttcatca gccacagttc cgatgcgttc
      961 agtttgccgt tgccacggag agcggcgatt ctggttccat cgggagagac gactccggag
     1021 tcggtttcat cggagatttt gtgggagggt ccgccgagga tggaaacatt ccacagactc
     1081 ggttgctcgc cctcagattg aacaccacca accagcaaat ggttgccgtc ggggaaccag
     1141 gacacgacat tgggtttcag ccccggcgga agccatagct gatgagaatc ctcacgagcg
     1201 atttcccgga ggaagagtcc gcgcgggtca gtgtacgcaa gaagccgcgc atcgggcgag
     1261 atggccgcgc ggtaaaccgg aatgtccgga gaatttgcgg tgagtttggc ttcgcgcggc
     1321 acctcggcgc gcgccgcgcg attcccaatg tgaaagccga ccgagatgca tgcgccggcc
     1381 aacacgacga ttgccgcgat ccagcgagcc ctgttggtgc tacgccagta agttggcggg
     1441 acgacggaga ttgccggcac gggcgcggcc aggggtccgg tcaccggagc gaggaatctg
     1501 tatccgcgcc tggccagagt ttcgacgaag cgtggattat cggccgagtc tcggagcacg
     1561 tcccgcagcc tcttaatggc ggcgtttagg ctatggtcaa agtcaacaaa tgtgtcagcg
     1621 ggccacaggc gccgtttcaa ttcctcgcgg gtgaccactt ccccgggtcg ttccagcaat
     1681 aacgcgagca cctggtaggg ctgctcctgg atcttgatct tgacgccgtt ccggcgtagc
     1741 tccccggcgc gagggtccac ttcataaact ccgaatctca cgacctccga taggttattg
     1801 ctggttggga cgctggccac cgggttcacc tttgatgaca ttagacgtca tctctcttac
     1861 ttatacgttc tacgaaggat ttcgttccac taagcctttc tcattttccg caaagtgaca
     1921 gggtatttct ggccgtaaac ctatgagtta cagcggttta ctgggttacc tacatttggg
     1981 cggagcgtca cccgcgaagc cttgtagcac gcaccggctc aggagacttt gcgccattcg
     2041 cgaggaggaa tcccgcaatg tggcgcaaat tccagttggc cagggccttt ggttggctgt
     2101 tcataagcct ccttccgccg gttgtgagtg cagctccaga cgcccacaat atgcccgtaa
     2161 gtctgacggt ttccatcttc aatgacgctg gcgtgccggc atccgtctta cgacaagccg
     2221 agttgcgtgc ccgtgcggtg ttaatggatg ccggcgttac gctcacttgg ctcgactgcg
     2281 gcgtacccgg tcaatggcga accaaactgg gctgccaggc gatagtcttt ccgacgcact
     2341 tctccgtgcg gttggtggcc ggccgacaag gggtatccga cgacaccttc ggacagtctt
     2401 acctgaacga acgcggcgag ggcaattacg cttacgttta tgtcacgccg ttaagctccg
     2461 caagagcgct aagtgtgatc aaagaaggcg atttgctggg atacgtggtg gcgcatgaac
     2521 tgggacattt gcttttggga aaggaatcgc attccgccgg ggggttgatg agggccaggt
     2581 gggaagtcgc ggaacttcag gaaggggcgc ggggcaaatt gctctttagc cgcggcgaag
     2641 cggaagagat ccgcggccgg tatctggcgg ccgcagctcg agcggggatt cttgtggccg
     2701 gggcgggcaa ataaagcacc gaccaaccag cacggcttag cggtttgcct tcacgacgcc
     2761 actgccttcg ttcagagtat tgatggagtc tggtcgaagc tcaacgaacg tttcggtcaa
     2821 gccgctaggc cagcggactt cgacccattc aggcttgctg atcgccccga gtccaaaatg
     2881 cacgcgcatg tcgttgttgg acacgtagct ggaaccgctg cgcacttcgt ccaccaaaat
     2941 ccgctcgcct gccttcacgc ggattttcgc gccaattccg tcgcgattgg attttgtgcc
     3001 gaccgtcttg aaagcgatcc agtgattcga agatcgctcc tgattgacca gcaggctgac
     3061 cggagcgttc atgttgctga tgaccgcgga gatgcggccg tcattccaga gatcgccgat
     3121 ggcgagtccg cggccggaag cagccgcggt aattgcagga cccgcgctgg cagaaatgtc
     3181 ttcaaatgtg ccgttgccct tgttgtgata aagaatgcgc ggctcgagat agtcgctgcc
     3241 gaggtgctgg ctgtctactt ccggatagac gtggccgttt accaggagca ggtcgggcca
     3301 accatcattg tccacgtcga ggaacatcgt tccccagccc agatactttg tgtacagacc
     3361 gaggccggcc gggaaagtga cgtcgtcgaa cgtgccgtcg ccgttgttgc ggtaaagtgt
     3421 cgccgtgtcg tcggaaaaat tggtcttgaa gatatccagg cggccgtcgc cattgtaatc
     3481 gccaatggta gagcccatgc cgg
//
LOCUS       DASTPM010000230         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_151821, whole genome shotgun sequence.
ACCESSION   DASTPM010000230 DASTPM010000000
VERSION     DASTPM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151821"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1004
                     /locus_tag="VFR42_04100"
     CDS             135..1004
                     /locus_tag="VFR42_04100"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEU0368365.1"
                     /translation="MSAALRAMRALSIPLPNGSRISVALTLPFAVIHLLALGVFFVKF
                     RWPYLVTCLALVAFRMFWVTAGYHRYFSHRSYKTSRAFQFVIAFMAMTSSQKGVLWWA
                     AHHRHHHRFSDQENDLHSPTLFGFFWSHVGWIISDKYNDTRLNYIADFAKFPELRWLN
                     KYHLIPPVALATVLILIGGWPLFVWGFCMSTVLLWHDTFTINSLSHLFGTRRYDTSDT
                     SKNNWLLALLTLGEGWHNNHHHYMASARQGFFWWEIDITYYTLKVLSWVGLVWDLRKV
                     PAHMLAEEQQMAA"
     gene            1490..2713
                     /locus_tag="VFR42_04105"
     CDS             1490..2713
                     /locus_tag="VFR42_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU0368366.1"
                     /translation="MSHGTKSGIGELAPPMRLMMTPGPSSVDPRVYRALSAPIVGHLD
                     PWFKTCMDETQVLMRQVFQTENRITMPLSASGSGGIEAAVFNTLEEGDEAIVCVNGYF
                     SGRMYETAIRTPAKITKVEAPLGRAVDPEDVRKAGAGRKIKVVGFAHGETSSGVVTAL
                     DPYRKVADELGALLVVDAVASLAAEPMHVDKQRLDIVFSGSQKALSAPPGMSPITISP
                     RAEEVLRNRKTRVQSWYFDLTTAMNYWGKDRLYHHTPPISLIYALHEAMRLTMEEGLE
                     VRWERHRLNQQALIAGVEAMGLRLFVENPKERLVTVTPVMVPAGVDDVKYRNQLLDEF
                     NIEIAGGIGPLKGKIWRLGLMGYCAQRANVLLLLAAMEQVLHAQGARVPAGAGVAAAI
                     RSYAEAETAVGAAKS"
     gene            2744..3118
                     /locus_tag="VFR42_04110"
     CDS             2744..3118
                     /locus_tag="VFR42_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368367.1"
                     /translation="MTTYPVLANNGAMSQFVGAFEVTSKLTGKVYQCRFSHMWNGIAT
                     RHADTIDTKFFVDGDALVVGLAHTAFIKFRATTGRDLSDREASFVSAQYLRERLEEED
                     ERKLYDVAGEDVTRLITQAGIK"
BASE COUNT          692 a          939 c          967 g          704 t
ORIGIN      
        1 taaactaaaa ttggttgcgt agtgcgaacg tccggccctg ttttgcgcgt ttcggactgg
       61 ctgcgcaaaa cttcccaccc cagggttgcc agaatcctgc gaaccgggca acggtacacg
      121 gaggaaattt cgggatgtct gctgcactac gcgcgatgcg cgccctttcg atacccctgc
      181 ctaatggatc ccgcatttcc gtagccttga cgctgccgtt cgccgtcatt cacctgctgg
      241 ccctgggcgt gttttttgtg aaatttcgct ggccgtattt ggtcacctgc ctggcgctag
      301 tggcttttcg aatgttctgg gtcaccgccg gctatcatcg ctacttttcg catcggtcct
      361 ataagaccag ccgcgccttc caattcgtga tcgcgtttat ggcgatgact tcatcacaaa
      421 agggcgtgct gtggtgggcg gcgcatcatc gccaccatca ccgtttttcc gatcaggaaa
      481 acgatttgca ctccccgacg ctatttggat ttttctggtc gcacgtgggc tggattatct
      541 cggacaagta caacgacacg cggctgaact atattgcaga cttcgccaag tttccggaat
      601 tgcgttggct caacaagtac cacctgattc cgcccgtggc gctggccacc gtgctgatct
      661 tgattggcgg atggccgttg tttgtgtggg gattctgcat gagcacggtg ctgctgtggc
      721 acgacacttt tacaattaac tcgttgtcgc accttttcgg cacgcggcgc tacgacacca
      781 gcgataccag caagaacaac tggctactgg cgctgctcac gctgggcgaa ggctggcaca
      841 acaatcacca ccactacatg gcttcggcgc gccagggctt cttctggtgg gaaatcgata
      901 ttacctacta cacgctgaag gtgctcagct gggtgggcct ggtttgggac ctccgcaagg
      961 tcccggcgca catgcttgct gaagagcagc agatggccgc gtagcgactg tttccaacac
     1021 ttttgccgag ataacgggca gcggcggtcg ctggtcccgt ttttgctcac cgggcagagg
     1081 gaaattgcct gatggcaatt gctggaaagg ccggcgaaag cgtcagcgct gggcgcgctg
     1141 cagaaaaggg cgatgatttg atcttcgggt ctgccggtga ccgcattagg gcttccgagt
     1201 tcgcaacgcg gccggcaccg gcttcgctga ctctggtacg gccgaacgac ctacgctggc
     1261 tattcctaga ccaccgggtc cggacagaga atccggacgc tcttagggtt gggcacaaat
     1321 caatacaatt tgtcgctgac tgacccgccc gtagagattt gaagcacctt ccgtatagcg
     1381 gccaatatgt cggttgcggg gctccggcaa ttgcgggaaa atctaggccc gcctaacaag
     1441 ccctgctgca ccggatggcg agctgaggtc gcgaattgag gtcgcatcga tgagccacgg
     1501 aacgaaatcg ggaattggag aattggcgcc acccatgcgc ctgatgatga cgccggggcc
     1561 gtcttcggtc gatccgcgcg tttatcgcgc gctttcggcg cccatcgttg gccacctcga
     1621 tccctggttc aaaacgtgca tggatgaaac gcaagtcctg atgcgacagg tttttcagac
     1681 cgaaaatcgc atcaccatgc cgctatccgc ttcaggttcc ggcggtatcg aagctgcggt
     1741 ctttaacaca ctcgaagagg gtgacgaagc gatcgtttgc gtgaacggct acttttctgg
     1801 gcgaatgtat gagaccgcga ttcgcacccc ggcaaaaatt acgaaggtgg aagctccgct
     1861 tggcagagcg gtggatcccg aagatgtgcg aaaagcaggc gcgggaagaa agattaaggt
     1921 cgtcgggttc gctcacgggg aaacttcttc gggtgtggtt acggcgttgg atccgtaccg
     1981 aaaagtagcg gacgagctgg gcgcattgtt ggtggtcgac gcggtagcgt cgctggccgc
     2041 agagcctatg cacgtggaca agcagcggct ggacattgtt ttcagcggct cccagaaagc
     2101 cttaagcgcg ccgccgggca tgtctcccat aactataagt ccgcgggccg aagaagtcct
     2161 gcgcaaccgc aagactagag tacagagctg gtacttcgac ctgaccaccg cgatgaacta
     2221 ctggggcaaa gatcgcctct atcatcacac gccgccgatt tccctgattt acgcgctgca
     2281 cgaagctatg cgcctcacga tggaagaggg cctggaagtt agatgggagc gccatcgcct
     2341 caatcagcaa gcgctcattg ccggcgttga agccatgggc ctgcgactgt tcgttgaaaa
     2401 tcccaaagag cgcctggtca ccgtcacacc ggtgatggtt cccgccgggg ttgacgacgt
     2461 aaaataccgc aaccagttgc tcgatgaatt caatattgag atcgcgggtg gtatcgggcc
     2521 gctgaagggc aagatctggc gcctcggcct gatgggttac tgcgcgcagc gagccaatgt
     2581 attattgttg ctggcggcca tggaacaagt gctgcacgcg cagggcgcgc gtgttccggc
     2641 gggcgcaggt gtcgcagcgg ccatacgcag ttacgccgaa gcggaaaccg cagtgggcgc
     2701 ggcaaaaagc tgagcgtaac gcgattcgat ttcaggagca ggcgtgacca catatccagt
     2761 cctagccaac aacggcgcca tgagccagtt cgtcggcgcc ttcgaggtca cctcgaagct
     2821 caccgggaaa gtttatcagt gccgtttttc gcacatgtgg aacggcatcg ccacccgcca
     2881 tgccgatacg atcgacacaa agttttttgt ggatggcgac gcgctggtcg tcggcctggc
     2941 acacacggcg tttatcaaat tccgcgccac gactggccgc gatctcagcg accgcgaagc
     3001 cagcttcgtt tccgcccaat atttgcgcga acgcctggaa gaagaagacg aacgcaagct
     3061 ctatgatgtc gccggggaag atgtgactcg tctgattacc caggccggca ttaagtaagc
     3121 cgtcagcgca agttttgcgt gccggttagc tgcgtcccac catctctggc tgctccacat
     3181 gaaacgcctc aagctgcgga tgccgcaatc catcgcgccg aataaagtga atcaccagca
     3241 gcatcagcaa ggctccgcaa accaaaatcc ccacggcaat ttccagcccg acttgtagat
     3301 cg
//
LOCUS       DASTPM010000231         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_469232, whole genome shotgun sequence.
ACCESSION   DASTPM010000231 DASTPM010000000
VERSION     DASTPM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469232"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="VFR42_04115"
     CDS             complement(<1..1431)
                     /locus_tag="VFR42_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0368368.1"
                     /translation="MAHRTTLEAERPAKTAKAGKKSASDANASKAGSSERERIFDAFR
                     RWGYNEANLDPLGFYGPFPQPDLEGLTGNIAAEARRLYCGTIGADFMHLPQPERRRWI
                     AERMESAAEPVDQDKILERLVRADLFEQVLQSRYLGTKRFSLEGVTALIPLLDSILDT
                     AGEHGAVESVMAMSHRGRLNVMVHAACKSPHEVVAGFEDVDPRSVLGAGDVKYHVGAT
                     GTYVTSEGKEIAIHLVSNPSHLEAVDPVAIGRVRAKQTRYGAASAEKVVPIVMHGDAA
                     FAGQGVWAETLNLADLQAYTVGGSIHIIVNNLIGFTTRPPQEQSSRFASDLAKRQYVP
                     VFHVNGEDPDAVVRVGKIAAEYRAQFGSDVVIDLVGYRRHGHSEVDDPTITQPRLYER
                     IKNHPPLWRIYSERIGVQPMQIVENVRKEYEEEQSKARQIKKIPLLRQLPDYWSAYHR
                     GRFKPEYEAETGLDREKLAEITDALVR"
     gene            complement(1513..>2158)
                     /locus_tag="VFR42_04120"
     CDS             complement(1513..>2158)
                     /locus_tag="VFR42_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0368369.1"
                     /translation="TVYYALVWPATQILGHPKIIGRENLKNLRGPVLVVSNHITRRAD
                     MGLILAALPRRFRHRLTTAMGGESLQRMRRPPREWFAAKRWAYQLGYRLVVALFNVFP
                     LPQFSGFRESFRYAGESADRGYSVLIFPEGEVNNTETGEMAPFQSGIGILADNLRIPI
                     VPMRLDGVWQMKRQHRRLARLGEITVHIGAPVTFPAGTPADEVARALESAVRNL"
BASE COUNT          398 a          596 c          715 g          449 t
ORIGIN      
        1 gcggacaaga gcgtcggtaa tctccgccaa tttttcgcgg tcgagaccgg tttccgcttc
       61 gtactcgggt ttgaagcggc cgcgatgata ggcggaccaa tagtcgggaa gctgccgcaa
      121 gagcggaatc tttttgatct ggcgcgcttt gctttgctcc tcttcatatt ccttgcgcac
      181 attttcgaca atctgcatgg gttgcacgcc gatgcgctcg gaataaatgc gccagagtgg
      241 cgggtggttc ttgatgcgct cgtacaagcg aggctgggtg atggtggggt cgtccacttc
      301 gctgtggccg tgacggcgat agccgacgag gtcgatcacc acgtcgctgc caaattgcgc
      361 gcgatactca gcggcgattt tccccacgcg caccacggcg tcgggatctt cgccgttgac
      421 gtggaaaacg gggacgtact ggcgcttggc caggtcagag gcaaatcgcg aggattgctc
      481 ctgcggcggg cgggtggtga agccgatcag gttatttacg atgatgtgga tcgatccgcc
      541 gacggtgtag gcctgcagat ccgcgaggtt gagcgtttcc gcccagacac cttgcccagc
      601 gaaagcagcg tcaccgtgca tgacgatggg gacaactttt tccgcgctgg cggcgccgta
      661 acgggtttgc ttggcgcgca cacggccaat ggccacagga tcgacagctt cgaggtggct
      721 ggggttggaa accaggtgaa tggcgatttc cttaccttca gaggtaacgt aggtgccggt
      781 tgcgccgacg tgatatttca cgtcgccggc gccgagcacg ctgcgcggat cgacatcttc
      841 aaatccggcg acgacctcgt gcggcgattt gcacgcggcg tgcaccataa cgttcaggcg
      901 cccgcgatgg ctcatggcca tcacggattc gacggcgccg tgttcgccgg cagtgtcgag
      961 aatggaatcg agcagcggaa tgagcgcggt cacgccttcc agggaaaagc gcttggtgcc
     1021 caggtagcgc gattgcagga cctgctcgaa aaggtcggcg cgaacgaggc gctcgagaat
     1081 tttgtcttga tcgacgggct cggccgcgct ttccatgcgc tcggcgatcc agcggcggcg
     1141 ttccggttgc ggcaggtgca tgaaatctgc gccgatggtg ccgcaataga ggcgacgagc
     1201 ttccgcggcg atgttgccgg tcaagccctc caggtccggt tgcggaaaag gcccgtagaa
     1261 accgagggga tcgagattgg cctcgttgta gccccagcgg cgaaaggcgt caaagatgcg
     1321 ctcgcgctcg gatgagccgg ccttcgaggc gttggcgtcg gaagcggatt ttttccccgc
     1381 cttcgccgtt tttgcggggc gttctgcttc aagggtggtt cggtgggcca taaattcgtt
     1441 cctgcaaacc tctattttgc cacagaacga agagctgtgc tttttgccgg cctgaggttt
     1501 gttttttcag gtttaaagat ttcgcaccgc ggattctaac gctcgtgcaa cttcgtccgc
     1561 tggcgtgcct gcggggaacg taactggggc gccgatgtgt acggtgattt cgccgaggcg
     1621 ggccaggcgg cgatgctggc gcttcatctg ccagacgccg tcgaggcgca tgggcacgat
     1681 ggggatgcgc agattatcgg cgaggatgcc aattccggat tgaaacgggg ccatctcgcc
     1741 ggtttccgta ttgttgactt cgccttccgg aaagatcagg acactgtaac cgcggtccgc
     1801 cgattcaccc gcataacgaa agctttcgcg aaagccggaa aactgcggca gcgggaaaac
     1861 gttgaagagc gcgacgacca gccggtagcc aagttgatag gcccagcgtt tggccgcgaa
     1921 ccattcgcgc ggcggacgcc gcatccgttg cagcgattcg ccgcccatgg cggtggtcag
     1981 ccgatggcgg aatcgccgcg gcagggctgc cagtatcagg cccatgtcag cgcgacgagt
     2041 gatgtgattg gagacgacca gcaccgggcc ccgaagattt ttcagatttt cccgaccaat
     2101 gattttggga tggccaagga tttgcgtggc gggccagacg agcgcgtagt acacagtt
//
LOCUS       DASTPM010000232         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_94366, whole genome shotgun sequence.
ACCESSION   DASTPM010000232 DASTPM010000000
VERSION     DASTPM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94366"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /locus_tag="VFR42_04125"
     CDS             complement(<1..1895)
                     /locus_tag="VFR42_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0368370.1"
                     /translation="MFYGREKALLRLIGLACAPALLPAVVQAQWKAKDTEWPTYAADL
                     AGTRYRALDQINASNFSDLEIAWRIKTDNFGNRPEYKLEGTPLMVNGVLYATAGSRRA
                     AIAVDAVTGELLWIHGEHEGARGGAAPRQLSGRGLAYWSDGKDERIFYVTPGYQLICL
                     DAKTGLRVASFADKGAIDLKQNDDQEIFPDLTTGEIGIQSAPVVAKNTIIIGAAFREG
                     MTPKSMHNNKGYVRGFDVRTGKRLWIFHTIPKKGEFGYDTWLNGSAEYTGNNGVWTQV
                     TVDEELGLVYLPVESPTNDFYGGHRHGNNLFGETLVCVDLKTGERKWHYQLVHHPLWD
                     MDISSAPILANITVDGKSVKAVAQPSKQGFLYVLDRVTGKPVWPIEEKQVEVGTVPDE
                     WYSPTQPIPSKPPAYSRNGVLESDLIDFTPELRERGKTIASKYKLGPVFTPPSESKVG
                     GPLGTLTVGTASGGTNWPGGSYDPETHTVYAFACNACAEPIGLVRAPKTVSDMNFIAG
                     TAGEEVTILRGPGENAGADSPMPPKKRSAGSGYVALNVDGLPLLKPPYGTISAINLDK
                     GEIIWQIAHGETPDVVRNHPALKGMNIPRTGQETYNIGTLVTKTLVIAGDGQITTTSE
                     HPRGAMLR"
     gene            complement(1951..>2248)
                     /locus_tag="VFR42_04130"
     CDS             complement(1951..>2248)
                     /locus_tag="VFR42_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368371.1"
                     /translation="LDHGMGAILAEGNNFTKLIANVFAAKRAGKEIDAPIAKLNTALK
                     LLRAGGVDEYGPMKYALSLQMGTRQFYQRPPNSDVSEEQKVKIKAALDEIKRMG"
BASE COUNT          400 a          712 c          610 g          526 t
ORIGIN      
        1 cgcagcatcg cgccgcgcgg gtgctccgac gtggttgtaa tctggccgtc gcccgcgatc
       61 actaaagtct tggttaccag cgtcccaatg ttgtaggttt cctgcccggt gcgcggaata
      121 ttcatcccct tcaatgccgg atggtttcgc acgacgtcgg gtgtttcgcc gtgggcgatc
      181 tgccagatga tttcgccttt gtcgaggttg atcgcgctga tcgtcccgta cggcggtttc
      241 aaaagcggca agccatccac attcagcgca acgtaaccgc tgcctgccga ccgcttttta
      301 ggcggcatcg gcgaatccgc gccggcgttt tcgccgggcc cgcgcaaaat tgtgacttcc
      361 tctcccgccg ttccggcgat gaaattcata tccgaaactg ttttcggcgc gcgtacgagt
      421 ccgatgggct cggcgcaggc gttgcacgcg aaggcgtata ccgtatgcgt ttcgggatcg
      481 tacgatccgc ccggccaatt tgtgccgccc gaggccgttc ccacggttaa cgtgcccagt
      541 ggcccgccca ctttgctttc gctgggtggc gtaaacaccg gtcccaattt gtacttggac
      601 gcgatggttt tgcctcgctc gcgcaactcc ggggtgaaat cgataaggtc gctctccaac
      661 acgccattgc ggctgtaagc gggcggcttc gaggggatgg gttgcgtggg tgaataccat
      721 tcgtcgggca ccgttcccac ttccacttgc ttttcttcaa tcggccagac cggtttcccc
      781 gtgacacgat ccagcacata cagaaagcct tgtttgctcg gctgggctac ggccttcaca
      841 gatttaccgt ccacggtgat gtttgcgagt atcggagcgg aagaaatatc catgtcccac
      901 aggggatgat gcacgagctg gtagtgccac ttgcgctcgc ccgtcttcag atcgacgcac
      961 accaaagttt cgccaaaaag gttattgccg tgccggtgcc cgccatagaa atcattcgtc
     1021 ggcgattcca ccggtaagta caccagtccc aattcttcgt cgacggtcac ctgcgtccag
     1081 acaccattgt ttcccgtgta ctctgccgag ccgttcagcc aagtgtcgta gccgaactca
     1141 cctttcttcg ggatagtgtg gaaaatccac aagcgcttgc ccgtccgcac gtcgaatccg
     1201 cgtacgtacc ctttgttgtt gtgcatgctc ttcggcgtca tgccctcgcg gaacgccgcg
     1261 ccgatgataa ttgtgttctt ggcgactacc ggagctgact ggatgccaat ctcgccggtt
     1321 gtcaggtcgg gaaaaatctc ctggtcgtca ttttgtttca gatcaatcgc gcctttgtcc
     1381 gcaaacgatg cgacccgcag ccccgttttt gcgtccaggc agatcagttg gtagccgggc
     1441 gtcacgtaga aaatgcgctc atcttttccg tccgaccagt aggcgaggcc gcgaccggaa
     1501 agctggcgcg gagccgctcc gccgcgagcg ccttcatgct cgccgtgaat ccacagcaac
     1561 tcccctgtga ctgcatccac cgcaatggca gcgcgccggg accccgcagt cgcgtacaac
     1621 acgccgttga ccatcagcgg cgttccctcc agcttgtact ccgggcggtt gccgaaatta
     1681 tcggttttga tccgccaggc aatttccaga tcgctgaagt tcgaggcgtt aatctgatcg
     1741 agcgcgcggt agcgggttcc cgccaagtcc gcggcgtacg taggccattc ggtatccttg
     1801 gccttccact gggcttgtac caccgcgggc aaaagggccg gtgcgcacgc caaaccgatc
     1861 agacgaagca gtgccttttc tctgccgtaa aacattccgc tcctcctcga ttcgcccagc
     1921 acagccttat ggcgttgtcc cctcgggcgc tcatcccatg cgcttgattt cgtccaatgc
     1981 cgccttgatc ttcaccttct gctcttcgct aacgtccgaa ttcggtggcc gctgatagaa
     2041 ctgccgcgtc cccatctgca aactcagcgc gtatttcatt ggcccgtact cgtcgacacc
     2101 gcctgcgcgc aggagtttga gcgccgtatt caatttcgca atcggcgcgt caatctcttt
     2161 gcctgcgcgc ttggccgcaa acacattggc gatcagctta gtaaaattat tgccctccgc
     2221 gagaatcgcg cccatgccat ggtccaga
//
LOCUS       DASTPM010000233         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1161316, whole genome shotgun sequence.
ACCESSION   DASTPM010000233 DASTPM010000000
VERSION     DASTPM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161316"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VFR42_04135"
     CDS             <1..1064
                     /locus_tag="VFR42_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0368372.1"
                     /translation="AQPPPPPQEKPQPTPPQQNTQQPTQEGPRFVTVVNLVDVLATVL
                     DRRNKLVPDLDKGDFKISDDSAQQEIRFFSKQSDLPLRIGMLLDTSNSIRDRIKFEQE
                     AAVDFLYSVLRRGKDQAFAMTFDDEPQVIQAFTGDGGMLRDEINKTRAGGGTAVYDAV
                     YEACAKQLSNPPRPPGDQPDIVRRVMILISDGEDNLSNHTRAEAIEMAQRTNVVIYTI
                     STSTQWIQLSQTDPSKAANRKMHLTEGDEILKNLAEETGGRAFFPYHIDDLNQSFQDI
                     GDELRNQYTITYSPSNFVANGKYHRIKIEIPNHNGYQVRARRGYFARSRTGPATPSQA
                     PAQNPSTSSPPTQTTTEKP"
     gene            complement(1090..>3230)
                     /locus_tag="VFR42_04140"
     CDS             complement(1090..>3230)
                     /locus_tag="VFR42_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368373.1"
                     /translation="EDLDARTDLFSLGAVLYEMVTGHHAFPGSTSAIIFDGILRNTPT
                     SPVTLNPSLPPEFERILNKALEKDRDVRYQVAAELRADLKRLQRESDSGRTPVAASAT
                     AAAASATASRSASTAAPAQPAKSSGSVIVAAAKANKLGTGLIAVLLVLLVAAAGYGGY
                     SLLKKPKHLPFENFAITNLTNSGHVSRAAISPDGKYLLQVHSEGGLQSLWLRHIATGS
                     NAQVVAPAATRYQALTFSRDGSYLYFLRRDEEEHIIGILYAAPVLGGTPHVVAKDVDS
                     PITFSPDGQRFAFLREKHDSPYWDLIVMKSDGSDGRQIFKDQNLLSDSFAPAWSPDGK
                     TIVIPVVQPSKDALGGLVQVDAATGKITNFVASTRIFYEPVWMPDGQGLIIPSLDLGS
                     GSQQSQLGYMTYPGGEYRLLTHDTNNYRNPSLAADGKTLVANQTKVTFDLAIAPAVSP
                     SQLKSVPLSSHEELWRWNWGANGKLILPQAGQVKSVGANGEETMLLNDTNYISDQTSA
                     CGDGRYIVFRRVSRAGTAAVHLWRMDANGNNVTQLTSGQNDREAVCTRDGKWVYYTDQ
                     TDHQTVKRVSIDGGAAQTIVSEPVGQFDLSADGKWIASVEVGEADHKLKIRLDPAAGG
                     KPTYADADARMSSPPLFGPDGKSIVYVAREKGVDNLWSQALDGTNRKQLTTFDKDFIF
                     RYEYSQDGKLIAIERGNIEADAFLLRDTSK"
BASE COUNT          660 a         1083 c          819 g          668 t
ORIGIN      
        1 gagcgcaacc acctccgccc cctcaggaga aacctcagcc aacgccacca cagcaaaaca
       61 cgcagcagcc cacccaggaa ggtccaaggt tcgttaccgt cgtcaacctg gtggacgtct
      121 tggcgactgt gctcgaccgc cgcaacaagc tggttcccga tctcgacaag ggcgatttca
      181 aaatcagcga cgacagcgcg cagcaagaga ttcgcttctt cagcaagcag tccgacttgc
      241 cgcttcgcat cggtatgttg ctcgacactt ccaacagcat tcgcgaccgc atcaagttcg
      301 agcaggaagc cgctgtggat tttctctaca gcgtgctgcg ccgcggcaag gaccaggcct
      361 ttgccatgac ctttgacgac gagcctcaag tcatccaggc cttcaccggc gatggcggca
      421 tgttgcgcga cgaaatcaac aaaacgcgcg ccggcggcgg caccgccgtg tatgacgcgg
      481 tctacgaagc atgcgccaag cagctcagca atcccccgcg cccgcccggc gaccagcccg
      541 acatcgtgcg ccgtgtgatg atcctcatca gtgacggcga agacaatctt tccaatcaca
      601 cccgcgccga ggctatcgaa atggcccagc gcacaaacgt ggtcatctac accatcagca
      661 ccagcaccca gtggatccag ctttcccaga ccgatccgtc aaaagccgcc aatcgcaaaa
      721 tgcacctcac cgagggcgac gagatcctga aaaatcttgc ggaagaaacc ggcggccgcg
      781 cctttttccc gtaccacatc gacgacttga atcaatcctt tcaggacatt ggcgacgaac
      841 tccgcaatca gtacaccatc acctacagcc cgtccaattt cgtcgccaac ggcaaatacc
      901 accgcatcaa aatcgaaatc cccaatcaca atggctatca ggtgcgcgcg cggcggggct
      961 acttcgcgcg gtctcgcacc ggaccggcca ccccatctca ggcgcccgcg cagaatccct
     1021 cgacttcgtc gccgcccaca caaaccacca ccgaaaaacc gtagcgggtg cttatccttg
     1081 ccagatttct tacttggaag tatcgcggag cagaaacgcg tccgcttcaa tgtttccgcg
     1141 ttcgatcgcg attagcttgc catcctgcga atactcgtaa cggaaaatga aatccttatc
     1201 aaacgtcgtc agttgcttcc ggttggtgcc gtccagggct tgcgaccata gattgtctac
     1261 gcctttttcc cgcgccacgt acacaatgga cttgccatct ggcccaaaca acggcggcga
     1321 actcatgcgc gcatccgcat ccgcatacgt cggtttaccc ccggccgcgg ggtccagccg
     1381 tatcttcagc ttgtgatccg cttcacccac ttcgacggac gcaatccact ttccatcggc
     1441 cgacaaatca aattggccca caggttcgct gacaattgtt tgcgctgctc cgccgtcaat
     1501 ggagacccgc ttcaccgtct gatggtccgt ctggtccgtg tagtacaccc actttccatc
     1561 tcgtgtgcat acagcctccc ggtcgttctg ccctgacgtt aattgcgtca cgttgtttcc
     1621 gtttgcgtcc attcgccata gatggaccgc cgctgttccc gcccggctca cccgccggaa
     1681 aacaatgtag cgcccgtctc cgcaagcgct ggtctggtcg gaaatataat tggtgtcgtt
     1741 cagcagcatc gtctcttcgc cgttcgcccc aaccgatttc acctgccccg cctgcggcag
     1801 aatcagcttt ccattggcgc cccagttcca tcgccacaac tcttcgtgcg aactgagcgg
     1861 cactgatttc agttgcgacg gagaaaccgc cggcgcaatt gccaagtcga atgtcacctt
     1921 cgtctggttt gccaccagcg tcttgccatc cgcggccagg ctcggatttc ggtaattatt
     1981 cgtgtcgtgc gtcagcagcc ggtattcgcc ccccggatag gtcatgtatc ccaattgcga
     2041 ttgctgtgaa ccggagccta agtccaggga gggaatgatc agcccttgcc catccggcat
     2101 ccacaccggt tcatagaaaa tcctggtcga cgccacaaag ttcgtgatct ttcccgtggc
     2161 cgcgtcgact tggaccagtc cgcccagcgc gtccttactt ggttgcacca ccggaatcac
     2221 gattgttttt ccgtccggcg accacgctgg tgcgaagctg tcgctcagca ggttttgatc
     2281 cttgaaaatt tgccgcccgt cgctcccgtc gctcttcatc acgatcaggt cccagtacgg
     2341 cgagtcgtgt ttttcgcgca aaaacgcaaa ccgttgcccg tccggtgaaa aagtaatcgg
     2401 gctgtccacg tctttcgcta ccacgtgcgg cgttccaccc agtaccggcg ccgcatacag
     2461 gatgccgatg atgtgctcct cttcatcgcg tcgcaaaaag tacaaatagc tcccgtcgcg
     2521 tgaaaaagtc agcgcctgat accgcgtcgc cgccggcgcc accacctgcg cgttgcttcc
     2581 ggtagcgatg tgccgcagcc acaacgattg caacccgcct tcggagtgca cttgcaacag
     2641 atacttcccg tcgggcgaaa tggccgcccg cgatacgtgt ccgctgttgg tcagattcgt
     2701 gatcgcaaaa ttttcgaacg gcagatgttt tggcttcttc agcagcgaat atccgccata
     2761 gcccgccgct gccaccagaa gcaccagcag cactgcaatc aacccggtgc ccagtttgtt
     2821 cgctttggct gccgctacga tcaccgagcc gctggatttg gccggttgcg ctggcgctgc
     2881 agtcgaagca gaccgcgacg cggttgcgct ggccgcagct gctgtcgccg aagccgccac
     2941 cggcgtccgc ccggaatcgc tttcccgctg cagccgcttc aaatccgctc gcagttccgc
     3001 cgctacttga tagcggacat cgcggtcctt ctccagcgct ttgttcagga ttcgctcgaa
     3061 ctccggcggc aggctgggat tcagcgtcac cggagaagtg ggtgtgttgc gcagaatccc
     3121 gtcaaagatg atcgcgctgg ttgaaccggg gaatgcgtga tgccccgtca ccatttcata
     3181 gagcactgcg cccagcgaaa acaaatcggt gcgcgcatcc agatcttcgc
//
LOCUS       DASTPM010000234         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_469299, whole genome shotgun sequence.
ACCESSION   DASTPM010000234 DASTPM010000000
VERSION     DASTPM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469299"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..658
                     /locus_tag="VFR42_04145"
     CDS             167..658
                     /locus_tag="VFR42_04145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0368374.1"
                     /translation="MDHISYTWYESPVGPLLLAGSHAALRYVSFSAGKHAVAIEPRWT
                     ENRNMFREAIRQLSEYFAGERKTFTLELGPPGTEFQRSVWTELRKIPYGETISYKQLA
                     ERIRKPKAVRAVGAANGANPIPIIIPCHRVIGHDGGLTGFGGGLPLKKRLLELESRQV
                     ALL"
     gene            complement(664..1815)
                     /locus_tag="VFR42_04150"
     CDS             complement(664..1815)
                     /locus_tag="VFR42_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0368375.1"
                     /translation="MRRIGIIFLLCALHTSTAFAQNTTQQQDRVVQLLKLLTEAPGPP
                     GFEEPVRKIMAEQMKPFADKISYDGLGSVIAQQGTSGPHIMLDAHMDELGGMVRRIRP
                     DGFVSMQMLGYWLTAALPDQRWVILGSKGPVLAVTDIWDAHIAPHDASGHTAQQDLYL
                     DTGARSAADVAALGISPGDPVAPVSDFAMLANNRYLAKAWDDRVGCAVMLEVMRRLQK
                     TSHPNQVFYAATVQEEGSIEMRGAQTSARLINPDIGFSLEVGIPNDVPGPGPEAAQEV
                     LGGGPGMMLYTFSELPNRKLVAHVKEIAAARQIPLQFDFVPGFGDDAGAIKLHNIGVP
                     VTTVLVPARSTHAHNGIIDRADFDRTVDLMVALIQSLDAATVAKLKSFE"
     gene            1959..2504
                     /locus_tag="VFR42_04155"
     CDS             1959..2504
                     /locus_tag="VFR42_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0368376.1"
                     /translation="MAIREILQLGDPRLRQAATLHDFAGDPADLQSLIVDLRDTLGHW
                     RKLTGYGRGIAAPQLGLSERVIFLQLAGAEPWPLINPQIMQRSEEKIVVWDACLSFLA
                     IFMQVERNKEVFVRYQNVQGEWRELRAGEERDLSELLQHEIDHLDGILCIDRVSDVTS
                     ICTKEEFEKRYRANSPYAAKV"
BASE COUNT          545 a          728 c          813 g          549 t
ORIGIN      
        1 gaatcttttt tcgaaaggga gttgaaggtc gtattttgtt tcgtcgcgac gcggccggga
       61 aggtggacgc gctgatcgac cggcggaaca acgaagacat tgtgtggaaa cgcatcagct
      121 aaaccacacg cgagcaagcc gtcgcaagac agattagact agaaccgtgg accacatttc
      181 ttatacctgg tacgagagtc cggttggtcc cttgcttctg gccgggtcgc acgccgcttt
      241 gcgctatgtg agcttttctg cgggaaaaca tgccgtggcg atcgaaccgc gctggacaga
      301 aaatcgcaac atgtttcgcg aagccattcg gcaattgtca gaatattttg ccggagagcg
      361 aaaaacgttc acgctggaac tgggtccgcc cggcacggaa tttcagcgat cggtctggac
      421 ggaacttcgg aaaattccct acggggaaac catttcttac aagcaattgg cggagcgtat
      481 ccgcaagcca aaggcggtgc gagcggttgg cgccgcgaat ggcgcgaatc ccattccgat
      541 tatcattccc tgccaccgcg tgatcggaca tgacggcggc ctcacgggct tcggcggcgg
      601 actcccgctc aagaaacgct tgctggagct ggaaagccgg caagttgcgc tgctgtgacg
      661 gctttattca aatgatttga gctttgccac cgtcgcggcg tcgagcgact ggatcaacgc
      721 caccatcaag tccacggtac ggtcgaagtc ggcgcggtcg atgattccgt tgtgtgcgtg
      781 ggtagatcgc gccggcacca ggacggtggt caccggtacg ccaatgttgt gcagcttgat
      841 cgcgccggca tcgtcgccga agccgggcac gaaatcgaac tgcagaggaa tttgccgcgc
      901 ggctgcgatt tcttttacgt gggcgaccag cttgcggtta ggcagctcgc tgaaggtata
      961 gagcatcatg cccgggccgc cgccgagcac ttcttgcgcg gcttcggggc ccggtcccgg
     1021 cacatcgttg ggaatgccca cttcaaggct gaaaccgata tcgggattaa tcaggcgtgc
     1081 cgaagtttgc gcgccgcgca tttcgatgct gccctcttcc tgaaccgtcg cggcgtagaa
     1141 gacctggttc gggtgagacg ttttctgcag ccgccgcatt acttccagca taacggcgca
     1201 gccgacgcga tcgtcccaag ccttggctaa atagcgattg ttcgcgagca tcgcgaagtc
     1261 agatacggga gccacaggat cgccgggaga aatgccgagt gcggccacgt cggccgccga
     1321 gcgcgcgccg gtgtccaggt acaaatcctg ctgcgcggtg tgcccggacg cgtcgtgcgg
     1381 ggcgatgtgc gcgtcccaga tgtcggtcac ggcgagcact ggacctttcg agcccagaat
     1441 cacccagcgc tggtcgggga gtgccgcggt cagccagtaa cccaacattt gcatggagac
     1501 gaatccatcc ggccggatgc ggcgtaccat gccgccgagt tcgtccatgt gggcgtccag
     1561 catgatgtgc ggaccggaag ttccttgttg cgcgatcacg gagccgaggc cgtcgtaaga
     1621 aatcttgtcg gcaaacggtt tcatctgctc ggccatgatt ttgcgtaccg gttcttcgaa
     1681 gcctggcgga cctggcgctt cagtcagcag cttgagtagt tgcactaccc gatcctgttg
     1741 ctgcgtcgtg ttttgcgcga atgcagtgct cgtatgcagt gcgcagagca agaaaatgat
     1801 tccgattcgt ctcaccgatg gtctcccggt gaacgatgat acggaaacat gttctttatg
     1861 gaaacgattt aatacggact tttcgcggcg gcggagtcgg tacaacgtgc cgtgccgagt
     1921 ggaagagagt aagattcgtt ttggggaggc gggttgaaat ggcgatccgc gaaattcttc
     1981 agctcggcga tcccaggtta cgccaggctg cgacgctgca cgactttgcc ggcgacccgg
     2041 ctgatttaca aagcttaatc gtggatctgc gcgacacact aggccactgg cgcaaactga
     2101 caggctacgg ccgcggcatt gccgcgccac aactgggcct ttccgagcgg gtgatatttc
     2161 tgcagctagc gggcgccgag ccctggccgc tgatcaatcc ccagatcatg cagcgcagcg
     2221 aagagaaaat cgtggtgtgg gacgcatgcc tgagctttct ggcaattttt atgcaggttg
     2281 aacggaacaa ggaagtcttc gtgcgctacc aaaatgtaca gggcgaatgg cgggaacttc
     2341 gcgcggggga agagcgcgat ctgtctgaac tcctgcagca cgaaatcgat catctggatg
     2401 gcattttgtg catcgatcgc gtgtccgacg ttacgagcat ttgcaccaag gaagaattcg
     2461 agaagcgcta ccgcgccaac agtccgtacg ccgcaaaagt ttgagctggt gtcttgtact
     2521 attcagaaga agcggcttca gcacctcgga gtttgcctgg gccgcccctt tgcaccaaca
     2581 ccgatcgggc cgtccgcaca ggttcgctgc gcttgaagcc gatcgacagg gaata
//
LOCUS       DASTPM010000235         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_757618, whole genome shotgun sequence.
ACCESSION   DASTPM010000235 DASTPM010000000
VERSION     DASTPM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757618"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VFR42_04160"
     CDS             <1..941
                     /locus_tag="VFR42_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368377.1"
                     /translation="LNVTASGAVRTTRYGASLDTSGNLSVQPVTRNDGEFTLEIRPPT
                     LERIFGAPSKKRRYKHTIGPEVTYRYVSGINDFERFIRFDSDATLSNTNEVEYGFTQR
                     LYVKNGDGQPTEFLSWNVVQKHYFDPTFGGAIADGERNVLQALNSITPFAFATGPRDW
                     SPIVSDFKVTPGGKYDAEQIIEYDPQLNKIVAEGTLVKVRPYSEFFATVAHFHIQDDP
                     VLQPLSNQIRALVGYGAFNRKGFNFTGGVSYDIQNSALQNQIAQVTYNGSCCGLSFEY
                     RRIALGQVRTENQFRVAFTIANIGTFGNLRRQEKIF"
     gene            complement(1192..2157)
                     /locus_tag="VFR42_04165"
     CDS             complement(1192..2157)
                     /locus_tag="VFR42_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0368378.1"
                     /translation="MQQSSWTAVLRRDLARTWASLGTFEHVALSYLALSSAMISVFSR
                     NLAHPWKLIGAQAIVATIIFLLCAAAARSEQRALRNGVTSSARFWHFWRHWYPHLFFL
                     FCFEELAYLVHLVNPNWQDAKLIAFDHWMFGVHPAVWLEQFATPLRNDLFQLAYLTYF
                     VYLLVLGGILYYRREWHAYWSVMTYSAVGYAIGYVIAALFPIQSPWFATAGMWQGELH
                     GGPFTATINFIEHYGRVRGAAFPSEHVAGSVAVVWGAWRHRRWLFWVMLPLVAAMCVS
                     TIWGRYHYVADIFGGMVTGTLGYFIGSWLVNRRPGLAPEIAPRTF"
BASE COUNT          542 a          621 c          690 g          419 t
ORIGIN      
        1 ggctgaacgt gacggcctcg ggagcggtgc ggacgactcg ttatggggct tcgctcgata
       61 catcagggaa tttaagcgta cagccggtga cgcggaacga cggcgaattc acgctggaga
      121 tccggcctcc gacgctggaa cggattttcg gggcgcccag caagaagcgg cgatacaagc
      181 acactatcgg gccggaagtg acgtaccgct atgtttcggg gattaacgat ttcgagcggt
      241 tcatccggtt tgattcggac gcgacgctct caaacaccaa tgaagtggaa tacggattta
      301 cgcaacggct ctatgtgaaa aatggcgacg ggcagccgac ggaatttttg tcgtggaacg
      361 tggtgcagaa gcattacttc gatccgacgt ttggcggtgc gatcgcggat ggcgagcgaa
      421 acgtgctgca ggcgctgaat tcgatcacgc cgtttgcatt tgcgacgggc ccacgggact
      481 ggtcgcccat cgtaagcgac ttcaaggtga ccccgggtgg caaatacgac gccgagcaga
      541 tcatcgaata tgacccgcaa ctgaacaaaa tcgtggcgga aggaacgctg gtaaaagtga
      601 ggccgtatag cgagtttttt gcgacggtgg cgcatttcca tattcaggat gatccggtgc
      661 tgcaaccact ttcgaaccag atacgcgccc tggtgggata cggagcgttc aaccggaaag
      721 gcttcaactt taccggcggc gtgagttacg acattcagaa ttcggcgctg caaaaccaga
      781 ttgcgcaggt tacctataac gggagttgct gcggactttc cttcgaatac cggcgcattg
      841 cgctggggca ggtgcgcaca gaaaaccagt tccgggtggc gttcaccatt gcgaatatcg
      901 gaacgtttgg gaacctgcgt cgccaggaga agattttcta gcgtactgcc cctgcgaacg
      961 acaagagcac attatttgca ggctgagccc cccatacagt catcccgccc cgcaatcctt
     1021 ccgattacgg tccccgtcgc agtcttggcc aaccagttca gcgttgacca tctgcggacc
     1081 ccgtggaccg cttgatcggc gcaaccacgc tcgcggaggg aattccgttg ctcaccaaag
     1141 atcgcaatat tttgaaaagc cgggcatttc gaacgatctg gtgatcccgg cctaaaatgt
     1201 gcgtggggca atttccggcg ccagtcccgg ccgccgattc acaagccacg agccgatgaa
     1261 ataccccagc gtgccggtga ccatgccgcc aaaaatgtct gccacgtagt gatagcggcc
     1321 ccaaatcgtg gagacgcaca tggcggcgac cagcggcagc atgacccaga aaagccagcg
     1381 acgatgccgc cacgcgcccc aaactacggc gacggaaccg gcgacatgct ccgagggaaa
     1441 agcagcgccg cgaacgcggc cgtagtgttc gatgaaattt atggtggcgg tgaacggacc
     1501 gccgtgaagt tcgccttgcc acatgccagc cgtggcgaac cacggactct gaatcgggaa
     1561 aagcgcggcg atcacatagc cgatcgcata gccgacggcg gagtaagtca tgaccgacca
     1621 atacgcgtgc cattcgcggc ggtaatagag gatgccgccg agaaccagca aataaacaaa
     1681 ataggttagg taggcgagct ggaagagatc gttgcggagc ggcgtggcga attgttcgag
     1741 ccacacggcc ggatgcacgc cgaacatcca gtggtcgaaa gcgatgagtt tggcgtcttg
     1801 ccaattggga ttcaccaagt ggacgaggta agcgagctct tcgaagcaaa aaagaaaaaa
     1861 cagatgcgga taccaatgtc gccagaaatg ccaaaagcgc gcgctggagg ttacaccgtt
     1921 gcgcagcgcg cgctgctccg agcgggcggc ggccgcgcaa agcaagaaga tgattgttgc
     1981 gacgatggcc tgcgcgccaa tcagcttcca gggatgcgca agattccggg agaagacgga
     2041 aatcatagcg ctggagaggg ccaaatagct cagggcgaca tgctcgaagg tacccagcga
     2101 cgcccaggtg cgcgcgaggt cccgtcgcag cacggcggtc cagcttgatt gttgcaatga
     2161 gaccagtgca cccatacgtg gtgacggata ttccctcaaa cttgtccgcc caatggatag
     2221 atgctctcgg gccggacttc gtgctgttct tgcatattta ttgctcagct cc
//
LOCUS       DASTPM010000236         6461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_584553, whole genome shotgun sequence.
ACCESSION   DASTPM010000236 DASTPM010000000
VERSION     DASTPM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584553"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1941)
                     /locus_tag="VFR42_04170"
     CDS             complement(139..1941)
                     /locus_tag="VFR42_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0368379.1"
                     /translation="MTASVRLRSWPSLVILVLLWLAVYVPGLVTPPLQDDVDASHADA
                     GQEILTRHDWVTLHENGIRYLEKAPLPYWGMAVGMKLFGPDAWSARLFLHLSVLANAF
                     LLYFFGRRFLTERAGFWAAVVFLACCGPYLFTRILIPDVTVGLWIGLILYLFLDGWQS
                     EKPTLLSCWSIAALIALNVLTKSLIGIVFPCAIIFLFLLLARDLRHLLKMHLVSSALV
                     FLLVAVPWHILAALRNPPAGESKGFLWFYFVNEQFLRYLNKRYPHDYNTVPLLLFYGL
                     LLLWFLPWSSFLPQALAQVSLRLPRVAGVRRSPRAVLLLLFCWPAIILLFFSFSTRQE
                     YYVAPGLPGLALIIGYWLARESEAPVSSPIARAGRRSALVLLVTGLVIAAITGFLALT
                     TRTPAPGSDIFDALNQNPNVYTLSLGHFLDLTSNAMAFFRGPLIGTALAFTIAPLLNW
                     FLRRRGQRAAANWALVAMMMVFIECAHIALGVFYPILGSKQFAVAIQQRLQPGELIVC
                     DGEYANASSVRFYTGQQLLILNGRINGLWYGSLFPDSPDIFLDDARFRKLWSGANRVY
                     LVTGDLKKRDSIAAVAPAYLLTKAGGRYVFSNRP"
     gene            2137..2865
                     /locus_tag="VFR42_04175"
     CDS             2137..2865
                     /locus_tag="VFR42_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU0368380.1"
                     /translation="MSSPTERQAVILAGGLGTRMRPITETIPKPMIAVAGKPFLEWQL
                     ALLRSGGIANALLLVAYLGEQIEEYFGNGAKTGGCVEYSYERSPLGTGGALKNAEAKL
                     RDWFVLVNGDTYLAIDYQKLMRDFVDADSRAMIVAYEKPAMATAAVPASGLPGNLGVS
                     SNGRVTAYRKREPEGLNHIDAGVIVLKKEVLLNLPMEKKCSLEEEIYPQLIARGEMRA
                     WVTTEPFYDMGSPAGLAALEAKLG"
     gene            2862..3875
                     /locus_tag="VFR42_04180"
     CDS             2862..3875
                     /locus_tag="VFR42_04180"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368381.1"
                     /translation="MILTRTPFRVSFAGGGSDLAEFYRRSTGAVLSCTIDKAMYIAVH
                     PYFHDKIRIKYSRTEDVATAAEVEHPLVRECLNLVGIERGMEIASFADVPAGTGMGSS
                     SAFTVGLLHALWAKSGRNPSAQELAAAACEIEIERAKEPIGKQDQYAAAYGGLNFIRF
                     NKDETVEVHPVKCLAKTRQELSRRLMLFYIGLERPAASILSEQGRNMANKDKFRRVEQ
                     MVELAVELRKSLERDSLDSFGDVLDQGWKLKRGLASGITNDVVERNYQLARKAGAAGG
                     KLLGAGSGGFLLLSCALQKQEQVRKALASLREMPVSLTETGSEVVHNDGMNGRAGVAT
                     GGN"
     gene            3953..4564
                     /locus_tag="VFR42_04185"
     CDS             3953..4564
                     /locus_tag="VFR42_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0368382.1"
                     /translation="MAGTKTNTVTAPSGFAAKEYLLAVQEVLERLDHAVIDQMVEVIW
                     RGYQAQRTLFLFGNGGSAALASHFACDIGKGTIAGRERRLKTIALTDNMALITAWAND
                     KSYDDIFSEQLDGLAEKGDIAMAISGSGNSPNIIRGLEMARKIGLQTLVLTGFAGGKA
                     KALGDLCLVAPSNSMQHIEDAHLCATHAIFLAIRERMKQANGG"
     gene            4554..5300
                     /locus_tag="VFR42_04190"
     CDS             4554..5300
                     /locus_tag="VFR42_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0368383.1"
                     /translation="MAVKQVHAAKKGPGANRQPVLKAVIFDLDGVVADSHPIHEIAWA
                     KLLVEQGLDRVTLNLDFLYAGHPRRAILKHYLGPISAADVEQLGRRKDELYGSAARLL
                     MPKPRIPETLRELNEDGIVCALATSAGRTRTYETLEKFGLTKSFATIVTGEEAGTPKP
                     APEIFLLAARRIETDPKCCVVVEDSVAGVAAARAAGMKCVGFAPEKRFRELAEAGADD
                     LISKLPADATSYFKSVLTAKSEEPAARARA"
     gene            5297..>6461
                     /gene="hpnI"
                     /locus_tag="VFR42_04195"
     CDS             5297..>6461
                     /gene="hpnI"
                     /locus_tag="VFR42_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEU0368384.1"
                     /translation="MIWLAYFLLAIALLGTLTSAIFLLLTIVGVFRFRGETRKFAGAK
                     VRDAELPPVSVLKPLHGMEAQLRENIESFFRQDYPRYEILFAADEADDPALNLVREIS
                     ARYPQIPCRILVTGKPPWPNPPAYCFYRMAEVAAHEILVTSDSDVEVAANYLREVVAP
                     ILDEKVGMVTCVYRGKNAAEFWAGLTAIGMSVEMTAGVLVANLLEGMKFGLGPTIAVK
                     KEAVNKIGGYEVLGDYFANDFMIGNLIDKAGYRVVLSEHVIDHVVSQKNFKKMWENQL
                     RWAKSTRYSRPKGHFGSGLIFAIPYGLLGLAAAILLGMPGLGSALFGVALVNRILESW
                     IVGWGVVRDPVAVKRIWMFPLRDLLGFIVWVASYTGATTAWRDSRYELRGDKIV"
BASE COUNT         1552 a         1510 c         2139 g         1260 t
ORIGIN      
        1 tgattctggt gtgcgccggg gtggcgctgg tggcggcagg ctaagcggca gtttcagtgg
       61 acaatatcca acttacatag caaaaggaat ttggcgaatc gaggtatcgc cgaattcgta
      121 tgctcggaca ccggcgaact aaggccgatt ggaaaacacg tagcggccgc cggccttggt
      181 gagcagatag gcaggcgcga cggcggcgat tgagtcgcgc tttttgaggt cgccagtgac
      241 aaggtacacg cgattggcgc cggaccagag tttcctaaag cgtgcgtcat cgagaaaaat
      301 gtccggagag tccgggaaaa gagagccgta ccagaggccg ttgatgcggc cgttaaggat
      361 gaggagttgc tggccggtgt agaagcgaac ggaagacgcg ttggcgtact cgccgtcgca
      421 aacgatgagt tcgccgggct gtaagcgctg ctggatggcg accgcgaatt gcttggagcc
      481 aaggatcgga tagaaaacac ccagagcgat gtgggcgcat tcgatgaaca ccatcatcat
      541 ggctacgagc gcccagtttg cggctgcgcg ttgaccgcga cggcgcagga accagttcaa
      601 aagaggggca atcgtaaagg ccagcgcggt gccaatgagg ggtccgcgga aaaaggccat
      661 agcgttgctg gtaagatcga ggaaatgacc tagagaaaga gtgtagacgt tgggattttg
      721 attgagcgcg tcaaaaatat cggagcctgg agccggggtg cgagtggtga gagcgagaaa
      781 tccggtgatg gcggcgatga ccagcccagt gacgagcaga accagagcgg agcggcgtcc
      841 ggcgcgggcg atcggtgatg agaccggagc ctcagattcg cgtgccagcc aatacccgat
      901 gatgagcgcg agaccaggga ggccgggagc tacgtaatac tcttgccggg tcgaaaaact
      961 gaaaaataga agaatgatgg cgggccagca gaagagcaac agaagaactg ctcggggtga
     1021 acggcgcaca cccgcaaccc gaggtaaacg tagacttacc tgagcaagcg cctgaggaag
     1081 aaacgaactc caaggaagaa accacagaag caaaagcccg tagaagagca gaaggggaac
     1141 ggtgttgtag tcgtgcggat agcgcttgtt taaatagcgg agaaattgtt cgttgacaaa
     1201 gtaaaaccag agaaagcctt tggactctcc ggcaggaggg tttcgcagcg ccgcgagaat
     1261 atgccaaggg accgcgacca gaagaaaaac caacgcgctc gagaccaggt gcattttcaa
     1321 gaggtgccgt aaatcacgtg ccaacaaaag gaacagaaaa atgatggcgc aggggaagac
     1381 gatgccgata aggcttttgg tgaggacgtt gagggcgata agggccgcga tgctccaaca
     1441 ggaaagcagc gttggctttt cggattgcca gccgtcgagg aagagataga ggatgaggcc
     1501 gatccagaga ccgacggtga cgtcgggaat gagaatgcgt gtgaacaaat aggggccaca
     1561 gcaggcaaga aagacgacgg cggcccagaa tcctgcccgc tcggtgagga accgcctgcc
     1621 gaagaagtaa agcaagaatg cgttagccaa gacactgaga tgcagaaaca ggcgcgcact
     1681 ccaggcgtcg gggccgaaaa gtttcatacc gacagccatg ccccagtaag gcagcggcgc
     1741 tttttcgagg taacggatgc cgttttcgtg cagggtgacc cagtcgtggc gggtaagaat
     1801 ttcctggccg gcgtccgcgt gagaggcgtc gacgtcatcc tgaagcggcg gcgtgacgag
     1861 gccgggaacg tagacggcga gccataacag aacgaggatt acgagcgacg gccaggaacg
     1921 cagtcgaaca gatgctgtca ttgactagga gtgcctcgga aggcgcgttt gtggcgcgaa
     1981 cgccttagaa aataaagaat agtttaccat gcggggcgct ggcgccaggc cgggaaggcc
     2041 gaaacggcat ggtattctga cgattacgct cgcgccagct gacagtcggt tcttttagac
     2101 ttgccgtggg gaaattccaa gagagtgaca ttgcggatga gttcgccaac ggaaaggcaa
     2161 gcggtgatcc ttgcgggcgg gctggggacg cgcatgcgtc cgatcaccga gacaattccg
     2221 aagccgatga tcgcggtggc tggcaagccg tttctcgaat ggcaactagc gttgctgcgt
     2281 agcggcggga tagcgaacgc tttgttgctg gtggcttact tgggtgaaca gatcgaagag
     2341 tattttggga atggtgcgaa aacggggggc tgcgtggagt attcatacga gcggtcgccc
     2401 cttggaacgg gcggcgcgct gaagaacgca gaggcgaagc tgcgcgactg gtttgtgctg
     2461 gtgaacggag atacgtatct ggcgatcgac tatcagaaat tgatgcgtga ttttgtcgac
     2521 gcggatagta gagcgatgat tgtggcttat gaaaagccgg cgatggcgac ggcggcggtt
     2581 ccagcaagcg gattgccggg aaacttgggt gtgagctcca atggaagagt taccgcgtat
     2641 cggaagcgag agcctgaagg gctgaaccac atcgatgcag gggtgattgt gctgaagaag
     2701 gaggttttgc tgaatttgcc tatggaaaag aagtgttcgc tggaggaaga gatctatccg
     2761 caattgattg cgcgaggtga aatgcgggct tgggttacga cggaaccttt ttatgacatg
     2821 ggaagcccgg cgggattggc ggcgctggaa gcgaagctcg gatgatcctc acgcggacgc
     2881 catttcgggt gagctttgcc ggaggcggca gcgacctggc agagttttac cgacgaagca
     2941 cgggagccgt gctgagctgc accatcgaca aggcgatgta catcgctgtg catccgtatt
     3001 ttcacgacaa aatccgcatc aaatattcgc gaactgaaga tgtagccact gccgcggagg
     3061 tagagcatcc gttggtacgc gaatgcctga atctcgtggg catcgaacgc gggatggaga
     3121 ttgcgagttt tgcggacgtg ccggcgggca cgggcatggg ttcgagcagc gcttttaccg
     3181 tgggcttatt gcacgcacta tgggcgaagt ctggtaggaa tcccagcgca caggaattgg
     3241 ctgccgctgc ctgcgaaatt gaaatcgagc gagccaaaga gccgatcggg aaacaagatc
     3301 aatatgcggc agcgtacggc gggctgaact ttatccggtt taataaagac gaaacggtag
     3361 aggttcatcc ggtgaaatgt ttggccaaga cgcggcaaga actctcgcga aggctcatgc
     3421 ttttctatat agggttggaa cggccagcgg cttcgatttt gagcgagcaa gggcgcaaca
     3481 tggcgaacaa agacaagttc cgccgcgtcg agcaaatggt cgagctggca gtggaacttc
     3541 ggaaatcgct ggagagagat agcctggatt cgttcggtga cgttctggat caggggtgga
     3601 aactgaagcg cggcttggcg agcgggatta ccaacgatgt cgtggagagg aattatcaac
     3661 tggcacggaa agccggggcg gcgggcggga aattactggg tgctggttcg ggcggatttt
     3721 tgttgctgag ttgcgccctg caaaagcagg aacaagttcg gaaagcgctg gcgagcctcc
     3781 gggagatgcc ggtaagtttg acggagaccg gctcagaggt tgttcacaac gacggaatga
     3841 acggcagggc cggcgtggcc acaggcggga actagcgcag gcagggaaca attgaaggcc
     3901 ccctgcggcc gagcgggtat ataattttaa gagccaccaa tgcggtgatt tcttggcagg
     3961 aacaaagaca aacacggtta cagctccgtc cggattcgcg gccaaggaat atctcctggc
     4021 cgtgcaagaa gtattagagc gactggacca cgcggtcatc gatcagatgg tggaagtgat
     4081 ttggcgcgga taccaggcgc aacggacatt gtttctgttt ggaaatggcg gaagcgcggc
     4141 gctggcgtcc cattttgcct gcgatatcgg caagggcacg atcgccgggc gtgaacgccg
     4201 gctgaaaacg atagcgctaa ccgacaacat ggcattgatt accgcgtggg cgaatgacaa
     4261 gagctacgac gatatttttt ctgagcaact ggacggactg gccgaaaaag gggatatcgc
     4321 gatggcgatc tccggcagtg ggaattcgcc caatatcatt cgcgggttgg aaatggcgcg
     4381 caagataggt ttgcagacgc tggtgctgac cggatttgcc ggcgggaaag cgaaggcgct
     4441 gggcgattta tgtctcgtag caccttccaa cagcatgcag cacattgaag atgctcacct
     4501 ttgcgcgacc cacgcgatct ttctggcgat tcgcgaacgc atgaagcagg ccaatggcgg
     4561 ttaagcaggt tcatgcggcg aaaaaaggcc ccggcgccaa ccgccagcct gtactgaagg
     4621 ccgttatttt tgacctcgac ggcgtcgtcg ccgacagtca tcccatacac gaaatagcct
     4681 gggcaaaact cttggtcgaa caagggctgg accgagttac gttgaatctg gattttctgt
     4741 atgcgggaca tcccaggcgg gccattctga aacactattt gggaccgatt agcgcggccg
     4801 atgtggagca gcttggtcgt cgcaaggacg agctttacgg ctctgcggcc aggttgctga
     4861 tgcccaaacc acgcattcct gaaacgctcc gcgagttgaa cgaagacgga attgtgtgcg
     4921 cgcttgcgac ctcggctggc cggacgcgaa cgtatgagac gctcgaaaaa ttcggcttaa
     4981 caaaaagctt tgcgacgatt gttacgggag aggaagcagg cactcccaaa ccggctcctg
     5041 agattttctt gttggcggcc agaagaatag agactgatcc aaagtgctgc gtggtggtgg
     5101 aagactcggt agcgggcgtg gcggcagcgc gagcggcggg gatgaagtgc gtaggatttg
     5161 ctccagagaa gcggttccgt gagcttgccg aagccggagc tgatgatttg atcagcaaac
     5221 tgcctgcgga tgcaaccagc tacttcaagt ccgttttgac cgcaaaaagc gaagagcctg
     5281 ctgcgagggc ccgcgcatga tttggctcgc ctattttctt cttgccatag cccttctggg
     5341 gacgctcaca tcggccattt ttctattgtt gaccatcgtt ggcgtatttc gattccgcgg
     5401 tgagaccagg aaatttgcgg gcgcgaaggt aagggacgcg gagcttccgc cagtgtcggt
     5461 attgaagccg cttcacggta tggaagcgca gctacgcgaa aacattgaga gcttttttcg
     5521 tcaagactac ccgaggtacg agattctgtt tgctgcagac gaggcggacg atcctgcgct
     5581 aaacctcgtg cgtgagatca gcgcgcgcta tccgcaaatt ccatgccgga tcctggtgac
     5641 cggtaaaccg ccatggccga atccgccggc gtattgcttt taccggatgg ccgaagtggc
     5701 ggcgcacgaa attctcgtga ccagcgacag cgacgtcgag gtcgccgcca actatctgcg
     5761 ggaagtagta gcgcctatac tggacgaaaa agtcggcatg gtgacgtgtg tttaccgcgg
     5821 caagaacgcg gcggaattct gggcggggct tacagcgatt ggaatgtccg tggaaatgac
     5881 cgccggcgtg ctggtggcga atttgctcga aggaatgaag tttgggctgg gcccaacgat
     5941 tgctgtgaag aaagaagccg tgaacaaaat tggcggctat gaagtgctgg gcgattattt
     6001 cgcgaatgac ttcatgatcg ggaatctgat cgacaaagca ggataccgcg tggtgctgtc
     6061 ggaacacgtt attgaccatg tggtcagcca gaagaatttt aaaaaaatgt gggaaaacca
     6121 gttgcgctgg gctaaatcca cgcgatattc gcggcccaag ggccatttcg gcagtggtct
     6181 gattttcgcg ataccttatg gattgcttgg attggccgct gcaattttgc tgggaatgcc
     6241 tggtctcggt tcggccctgt ttggagtcgc gctggtgaat cgcatcctcg aatcgtggat
     6301 tgtgggatgg ggcgtggttc gtgatcccgt cgctgtgaaa cggatttgga tgttccccct
     6361 gcgggatttg ctcggtttta ttgtgtgggt cgccagctac acgggagcca cgaccgcctg
     6421 gcgcgacagc cggtatgaat tgcgcggcga caaaattgtg c
//
LOCUS       DASTPM010000237         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_354313, whole genome shotgun sequence.
ACCESSION   DASTPM010000237 DASTPM010000000
VERSION     DASTPM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354313"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..494)
                     /locus_tag="VFR42_04200"
     CDS             complement(123..494)
                     /locus_tag="VFR42_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0368385.1"
                     /translation="MILQFLRHELSPGVAALEIKGNVHCGPECIRLEREVDAMVKDNL
                     TCVVFDLKSVTHMDSAAIGAIVRCHTKLKNAGGALRLASAQPMIAHSLQITKVDRIIK
                     VFPTIPDATQGFAPSASASPA"
     gene            complement(491..793)
                     /locus_tag="VFR42_04205"
     CDS             complement(491..793)
                     /locus_tag="VFR42_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368386.1"
                     /translation="MSEWVYIQAEPGEQLMQLHSFSFIKKQATGDVQFSITVKEFAVP
                     PPGQRVRFFAEADKQVNQKSGAFVPCGWGTTLFSALGDCVRLIRQFPYDQEGSSAA"
     gene            complement(1012..1344)
                     /locus_tag="VFR42_04210"
     CDS             complement(1012..1344)
                     /locus_tag="VFR42_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368387.1"
                     /translation="MISGMPQLVFIHVVISLPGAWSGITVWFGFFGNKRLDAGTHFLL
                     MPPPSPASPDFFFLRSSCRLPTSSAFSRWSFSPSPALLATAKKKCRGWHKTYAVAAMI
                     SLPSTSSC"
     gene            complement(1511..>2755)
                     /locus_tag="VFR42_04215"
     CDS             complement(1511..>2755)
                     /locus_tag="VFR42_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEU0368388.1"
                     /translation="FLTTTPNAAPVDAQAVFIGYGLKIPEANYDDFAGLDLKGKIAVY
                     ITGGPASIPTSVKAHYQSGEERRKHLAAAGAVGLITIPNPKAEEVPWSRVAGARFSQR
                     MELRDAAGAPAPQFTLVVNPEHAEKFFSGSGHTFSELLALLGSDKPLPHFPLNIIVRA
                     NTAVKRSTAQSQNLAGVLPGNDPQLKNEYVVISAHLDHLGIGQPVNGDKIYNGAMDDA
                     SGIASLVEVARAMRESNAQPKRSILFLAVTAEEKGLLGSQYYASHPTVKGPIVADLNM
                     DMYLPLFPLRYLEVQGLSESTLGDNITEVCKESGITVQADKQPDHVRFIRSDQYSFIK
                     KGVPSLAFKFGWLPGTPEEKLFNDWYKDRYHGPADDTSQPVDKAAAAKFDAILEKLAL
                     RVADADSRPTWKPDSFFRRFAQ"
BASE COUNT          584 a          641 c          984 g          546 t
ORIGIN      
        1 ccgaagaggt tagcgcgaac gggtgcgtaa tgggtgagat gcgctcagaa gagctctggt
       61 gtcgctggac gaaagccgga gcgtggcttc gggttcgaaa cagcgacagc aatgcgaact
      121 gcctaagcgg ggctggcgct agcggatggg gcaaaaccct gggtggcgtc ggggatagtg
      181 ggaaagactt tgatgatgcg gtcgacttta gtgatttgca ggctgtgggc gatcatcggc
      241 tgagcggaag cgaggcgcag cgcgccgcca gcgtttttga gcttggtgtg gcagcgaaca
      301 atggcgccga tggcggcgct gtccatgtgg gtgacacttt tcagatcgaa cacgacgcag
      361 gtaaggttgt cctttaccat ggcgtccact tccctttcaa gacggataca ctcggggccg
      421 cagtgaacgt tgcctttgat ttccagggcg gccacgccgg ggctgagttc gtggcgaaga
      481 aattggagaa tcatgccgcg gagctccctt cttggtcgta gggaaactgg cgaatgaggc
      541 gaacgcaatc gccgagcgcg gaaaacagag tagtgcccca tccgcagggg acaaacgctc
      601 cggatttctg attaacctgt ttgtcggctt cagcgaaaaa gcggacgcgc tggccaggcg
      661 gcggcacggc gaactccttg acagtgatgg aaaactgcac gtcgccggtg gcctgcttct
      721 tgataaagga aaacgagtgc aactgcatta actgctcgcc gggctcggcc tggatgtaaa
      781 cccactcgct catttgctca ccccgatgtg tgccccgata ggtgaaagcg atgctaggac
      841 agcggctgca gcctgtcaac gatgcctcag gccgggtgga ttttttgacc gcaaagacgg
      901 ggcggatgat gaagagaacg aggttgaagc ctggcgtgac ggcgaagggc ggcgcggatc
      961 cgctgggggc gagcccgtgc aacgcgggaa ctcttatgaa ggattgtgcg atcaacacga
     1021 agacgttgaa ggtagagaga tcatcgccgc tacggcgtag gttttgtgcc agcccctgca
     1081 tttttttttt gcagtagcca gcaaggcagg cgatggcgag aacgaccagc gggagaacgc
     1141 cgaggatgtg ggaaggcggc agctgctgcg caggaagaag aaatccggcg atgctggtga
     1201 gggtggtggc atgagaagga aatgggtccc ggcatcgaga cgcttgttgc cgaagaagcc
     1261 gaaccagacc gtgatgcccg accaggctcc gggcaggctg atgacaacgt ggatgaacac
     1321 cagttgtggc atgcccgaga tcacggctgc gattctccga atggggaaag agtaagagcg
     1381 tttgaagcgg acacaaccca cgaaataagc ggatgcgggt tcgagaaagc gatggcgggc
     1441 ggagatgcaa ggttttctcg cgcagaggcg cagagaacta acgcagagcc ggttgcagcc
     1501 cggttacaag ctattgagcg aagcggcgga agaagctgtc gggtttccag gtgggacgcg
     1561 agtcagcgtc ggctacacgg agcgcaagtt tttcgaggat ggcgtcgaat tttgcggcgg
     1621 cggccttgtc caccggttgt gaagtgtcgt cggcggggcc gtggtagcgg tctttgtacc
     1681 aatcgttgaa aagcttttct tcgggagtgc cggggagcca gccgaacttg aaagccagcg
     1741 aagggacgcc ttttttgatg aaactgtatt gatcgctacg aatgaagcgg acgtggtcgg
     1801 gctgtttgtc ggcttgcact gtgatgccgg attccttgca gacttccgtg atgttatcgc
     1861 ccagtgtgga ttcagagagt ccctgcactt ccagatatct cagcggaaaa agcggaaggt
     1921 acatatccat gttgaggtcg gcgacgatcg ggccttttac agtgggatgc gaagcgtagt
     1981 actgcgagcc tagaaggccc ttttcttccg cggtgacggc gaggaacaga atggaacgct
     2041 tgggctgggc gttcgattcg cgcatggcgc gagcgacttc gacgagcgac gcgataccgg
     2101 aagcgtcgtc catggcgccg ttgtagattt tgtcgccgtt gacgggctgg ccgatgccga
     2161 ggtggtcgag atgcgcgctg atgacgacat attcgttttt gagttgggga tcgttgccgg
     2221 ggaggacgcc agcaaggttt tgcgattgag cggtggaacg tttgacggcg gtattggcgc
     2281 ggacgatgat gttcagcggg aaatgcggaa gaggtttatc ggaaccgagg agcgcgagga
     2341 gttcggaaaa agtgtggccg ctgccggaga aaaacttttc ggcgtgttcg ggattgacga
     2401 ccagcgtgaa ctggggagcg ggagcgccag cggcgtcgcg cagttccatg cgctgagaga
     2461 aacgcgcgcc ggcgacgcgt gaccagggca cttcttcggc tttgggattg ggaatggtga
     2521 tgaggccaac ggcgccggcg gcggcgagat gtttgcggcg ctcttcgccg gactggtagt
     2581 gggctttgac ggaagtggga atggaagcgg ggccgccagt gatgtaaacg gcgattttgc
     2641 ctttgagatc gaggccggcg aaatcgtcgt aattggcctc gggaatcttg agtccgtagc
     2701 cgatgaagac ggcttgcgcg tcgacaggag cggcgttggg agtggtggtg agaaa
//
LOCUS       DASTPM010000238         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_325456, whole genome shotgun sequence.
ACCESSION   DASTPM010000238 DASTPM010000000
VERSION     DASTPM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325456"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..558)
                     /locus_tag="VFR42_04220"
     CDS             complement(85..558)
                     /locus_tag="VFR42_04220"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HEU0368389.1"
                     /translation="MQMKREWMAGAVLFSAMVLAILIPDGARSAAQQNGADEPVPAYH
                     TQPPAGEPPQTMDPAEFDTAVVKNSYALAAKVKKVLYQQPCYCHCDRSVGHGSLLDCF
                     TSKHGSMCNVCMGEALYSYEQTKKGKTPAQIREGIQKGEWKQIDAAKYQTYPVKP"
     gene            complement(631..>2097)
                     /locus_tag="VFR42_04225"
     CDS             complement(631..>2097)
                     /locus_tag="VFR42_04225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0368390.1"
                     /translation="GAILVVDDESEIREGLELLLTGEGYGVSSADTGESGLGKLEERP
                     FDLMLLDVSLPDRNGLDLLKEVRRRDPGLPVVLITAYGSIDMARAAFKGGALDYITKP
                     WSNDELLAQVAQAVESRRLREENVQLKRALKQRSSFPSIVGKSEKMLTLFDLVAQVAP
                     SRSTVLISGESGTGKELIAKAIHSASPRADKAFVPVNTGSIPVDLLESQLFGHVKGAF
                     TSAVSSKKGLFEVADQGTIFFDEIATISPETQAKLLRVIQEREFMRLGGTEQIKVDVR
                     IVAASNIELLTLVREGRFREDLYHRLNVIHLTLPPLRDRREDVPMLLAHFLVRYCEEN
                     GKPMRQFTPAAMKLMMDYDWPGNVRELENVVERAVVLSITERVDADLLPESIRSKEIV
                     KGVRLQLSEFMPPLPGEPGARTGADQPQHPSLFQIMDEIERRMIVDMLDRSGWNQTEA
                     AERFLIPLSTLNQKIKRLGIDVRRRGRGDDSFTASSGK"
BASE COUNT          405 a          674 c          539 g          479 t
ORIGIN      
        1 ggtagcgtcc cgttcaaagt ttcgaggcag gcgggtttcc ggaaattcta ctttgaacct
       61 taaactttcg atcttgaact gtctttacgg cttcacggga tacgtctgat acttggcggc
      121 gtcgatctgc ttccactcgc ccttctggat cccttcgcga atctgcgctg gcgtcttgcc
      181 cttcttggtt tgctcgtagg agtacagcgc ctcgcccatg cagacattac acatcgatcc
      241 gtgcttgctc gtgaagcagt ccaacaaact tccgtgaccc acgctgcggt cacagtggca
      301 ataacacggc tgctggtaca ggactttctt caccttggcc gcgagtgcgt aagaattttt
      361 caccaccgca gtgtcgaact ccgcgggatc catagtctgc ggcggctcgc cggctggtgg
      421 ctgcgtgtga tacgcgggaa ccggctcgtc cgcgccgttc tgctgcgccg ccgatcgcgc
      481 gccatcggga atcaaaatcg ccagcaccat ggccgaaaac aggaccgcgc ccgccatcca
      541 ctcgcgtttc atttgcattg gctccctccc gttgcgcgaa aaatgtcgca gcccattttc
      601 cgaatttcga atttcgcgtt tttttctgcg ttacttgccc gaagacgccg taaacgaatc
      661 gtcgccacgt ccgcgccgcc gcacatcgat tcccagccgc ttgatctttt gatttagcgt
      721 cgaaagcgga atcagaaacc gttccgccgc ctccgtctga ttccatccgc tgcgatccag
      781 catgtccacg atcatacgcc gttcgatttc gtccatgatc tggaacaaag acggatgttg
      841 cggctggtcc gcgccggttc gcgccccggg ttcgccaggc aacggcggca tgaactccga
      901 aagctgcagc cgcacacctt tcacaatttc cttgctgcga atgctttccg gcagcagatc
      961 ggcgtcgacg cgctccgtga tcgagagcac taccgcgcgt tccacaacat tttccagttc
     1021 gcgtacgttg cccggccaat cgtagtccat catcagcttc atcgcggccg gcgtgaattg
     1081 ccgcatcggc ttgccgtttt cctcgcaata ccgcaccagg aaatgcgcca gcagcatcgg
     1141 cacatcttcg cggcgatcgc gcaagggcgg cagcgtcagg tggattacat tcaggcggtg
     1201 atacaaatct tcgcggaagc gcccttcgcg caccagcgtc agcaattcaa tgttcgacgc
     1261 agctacgatg cgaacatcca ccttgatttg ttcggtcccg ccgaggcgca tgaattcgcg
     1321 ctcctggatc acgcgcaaca gctttgcctg cgtctccgga ctgatcgtcg caatctcatc
     1381 aaagaaaatg gttccctgat cggcaacttc aaacagtccc ttcttggaag agacagcgct
     1441 ggtgaacgcg cctttcacat ggccaaacag ctgcgattcc agcaggtcca ccggaatcga
     1501 gccggtgttc accggcacaa acgctttgtc cgcgcgcggc gaagccgagt gaatggcttt
     1561 ggcgatcagc tctttacccg tgccgctttc tccgctgatc agcactgtgg aacgtgacgg
     1621 tgctacctgc gcgaccaggt caaacagagt cagcatcttt tcgctttttc cgacgatgct
     1681 cggaaaactc gaacgctgct tcagtgcccg tttgagctgc acgttttctt cgcgcagccg
     1741 ccgcgattcc accgcctgcg ccacttgtgc cagcaattcg tcgttggacc acggtttggt
     1801 gatgtaatcg agcgcgccgc ctttgaacgc cgcgcgcgcc atatcaattg agccgtatgc
     1861 ggtaatcaac actaccggca ggccagggtc gcgccgccgc acttctttca gtaaatcgag
     1921 tccattacgg tcgggcagac tgacgtccaa cagcatcaga tcgaacggcc gctcttccaa
     1981 tttccctaat ccggactcgc cggtgtcggc gctcgacacg ccgtagcctt cgcccgttaa
     2041 cagcaactcc agaccttctc gaatttcaga ttcgtcgtca acgacgagaa tcgcacc
//
LOCUS       DASTPM010000239         5869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1363285, whole genome shotgun sequence.
ACCESSION   DASTPM010000239 DASTPM010000000
VERSION     DASTPM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363285"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1520
                     /locus_tag="VFR42_04230"
     CDS             270..1520
                     /locus_tag="VFR42_04230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0368391.1"
                     /translation="MSTNPDVLIIGAGLAGLSCGRHLADCGISVQIIETSDGIGGRVR
                     TDEHEGFLLDRGFQVLLTAYPEAQRTLDYHALDLKKFRPGAYSWFAGRMNELVDPWRT
                     AGGWSTAFKSDCGTLWDKLRIMRLRTRLRRSTIEQLFQRPESSTKDALKAAGFSDEFI
                     HRFFRPFFGGILLDGEMKSSSRMFEFIFKMLSEGDIAVPARGMGEIPAQVASHLPENS
                     VRLNAHAESLHENELTLAGGETLSARAIVVAADGPGAAHLVGEAEPASRSVTCFYYSA
                     DESPMPHPTIVLNGDGAGPVNNFAVMSQVAPSYAPARKSLIAISVLGTQQLTEDQLGG
                     FIIAQMKNWFGPVARSWRYLKSYRIPHAQPQQFPGALEPPQRPVRIRPGIYLCGDHRD
                     NASIHGAMISGRRAAEAVLADLAR"
     gene            complement(1545..2687)
                     /locus_tag="VFR42_04235"
     CDS             complement(1545..2687)
                     /locus_tag="VFR42_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEU0368392.1"
                     /translation="MTIRKIFFWLHLSAGIVAGSVILAMCLTGAALGFEKQIVHWAQR
                     HERTVAASDGAQLLSMDALLAQALPRESDQSVSVTWRSQPNATVELAYGRSRTVFLNP
                     YNGQILEDRAVQLRSFFDDTENIHRWLGVSGANRPTARAITGAANLLFLFLGCSGLYL
                     WRPRKWTWNALKTGTTLRRGLSGRARDFNWHNAIGFWMCLPLFVIVVCSTVMSYQWAN
                     NLVYRLSGSPVPLANAAPRPAGVRAGPQEKLKIEGLASLCALAERKVPEWQSITMRLP
                     AGKSANVIFQIDAGDGGRPDKRAQLTLDRETGDEVRWEPFSENTRGRKIRAWMRFAHT
                     GEAGGLLGQSIASAASLGGVFLACTGISLAIRRFLGVWSKAKPSDL"
     gene            complement(2707..3387)
                     /locus_tag="VFR42_04240"
     CDS             complement(2707..3387)
                     /locus_tag="VFR42_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004775794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEU0368393.1"
                     /translation="MLLAIPDVLSAAQVSQARQILDSALWVDGKVTAGHQSAQVKDNQ
                     QLREGSAESQQLGELILTALGQNAMFISAALPLRVFPPLFNKYTGGQSFGTHVDNAIR
                     QVPGTGHRIRTDLSATLFLAQPEEYDGGELVAEDTYGVHSVKLPAGHMVLYPSTSLHH
                     VRPVTRGARIASFFWIQSMVRDDGERTLLFDLDAAIQRVNRDAPASPSAVQLTGIYHN
                     LMRRWADA"
     gene            complement(3426..>5869)
                     /locus_tag="VFR42_04245"
     CDS             complement(3426..>5869)
                     /locus_tag="VFR42_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEU0368394.1"
                     /translation="VDRPDRSKSIKINRVDHWPVTYRWAAMGTLLAYSALGVTRVASA
                     APCAEARGKDGTPLGPQALVVRRFEIASSTLGDTMDAFAKVCGISVKFADDELRTLPS
                     KGVMGLYTSDQALKLLVADSGASYRFVAADAVSIQLAQVATSVEVSTDGAQMIASAKY
                     SELLRDTPQTISVIDNQILEQQGVNTLRDALRNVAGISLAAGEGGAQGDNLTIRGFSG
                     RNDIFLDGMRDFGSYYRDPFNYESVDVLQGPSSMTFGRGSSGGVVNQETKAPEVAKLL
                     GGTLQFGSDLTRRITADVNEPIGPTGAFRLNVMGHDSQVADRDIAENRRFGVAPSLAF
                     GLGTTTRLVLSYYHQQADDTPDYGIPWLFNGPSPVDRSNYYGFRDGNYLRTGVDIATA
                     KGEHDFGTAVTLRNQVRYGHYKRDVLITEPRIAGTPDPSTPLEDISVARNEIAVRSLE
                     TYLGDQLDLTFRFRTGFAEHTVVTGVEGGRETSSPMRFAWSGVPGTSLLNPDQNQELS
                     GTAAVSSDVKAMAISFGAYAIDTVKLSRQWSVIGGLRWDRFDTDYSQSVAPVSSFNRV
                     DQKPSYRAAVVYKPKPYGSIYFDYGTSFNPSAESLSLSASNANLPPENNQTFELGTKW
                     DFPGKRLSVEGAVFRTDKLNAREPDPNNPLLNVLAGEQRVNGAQVSVTGKITDGWQVL
                     GSYAYLDGKLVSSQFYPQAVGAQLANVPKNTFNLWTTHALPWKLTVGGGAQFVDSRTA
                     STTVPIDPFTGLVKQVPSYWLFNAMASRPVTEHLGVQLNLYNLANREYINEIHPAHLV
                     PGAGFTALAGLNFKF"
BASE COUNT         1160 a         1746 c         1747 g         1216 t
ORIGIN      
        1 cggcgcgaaa tcccaaacgc gcaacgtctc gagctttgat acacgcatgg atatcgtgtt
       61 gaacctgaaa ctgtcgccca agaagagttg aaggcgctcc tccacctgat tgcccctccg
      121 ctatctggca tcgaataacg caagaacgtt gctctacatc agcgcttgcg cagatcagcc
      181 agcttgccgc accatattcc cttcgcccag ggtcgttggc gataccgcgt atccatcctt
      241 caccaggcaa tcgggagtaa ctcggcagaa tgtccaccaa tccggacgtg ttgattatcg
      301 gcgcgggtct ggccggccta tcctgcggcc ggcatctcgc cgactgcgga atttccgtac
      361 agattattga gacgtcagac ggcattggcg gacgcgtgcg tacggatgag cacgaaggct
      421 tcctgctcga tcggggcttt caggtgctgc tcacggcata tcccgaagcg cagcgtaccc
      481 ttgactatca cgcgctggac ctgaagaaat tcaggcccgg cgcgtattcg tggttcgccg
      541 gaagaatgaa tgagctggtt gatccatggc ggactgccgg cggctggagc acggctttca
      601 aatccgactg cggcacgctg tgggacaagc ttcgcatcat gcggctgcgc acaagattgc
      661 ggcgatcgac gatcgagcag ctcttccagc ggccagaatc ttcgacgaag gatgctttga
      721 aggctgccgg cttttccgac gagttcatcc atcgcttttt tcgcccgttt tttggcggta
      781 tcttgctcga tggcgagatg aaatcctcta gccggatgtt tgagtttatt ttcaagatgc
      841 tttccgaggg agatatcgcg gtgcccgcgc gcggcatggg cgagataccg gcacaagtcg
      901 cctctcacct gccggaaaat tcggttcgct tgaatgcaca tgcggagtcg ctgcacgaaa
      961 acgaattgac tcttgcagga ggcgaaacgc tgagcgcgcg ggccatcgtt gtggctgcgg
     1021 acggcccggg agcggcgcac ctggttggcg aagcggagcc cgcgtcgcgc agtgtgacct
     1081 gtttttatta ttccgcggac gaatctccga tgccgcatcc gacgattgtg ttgaacggcg
     1141 acggcgcagg accggtgaac aattttgcgg tgatgagcca ggtagctccg tcgtatgccc
     1201 cggcgcgaaa atcgctgatc gcgatcagcg tgctcggcac gcagcagctg accgaagacc
     1261 agctcggcgg attcatcatc gcgcagatga agaactggtt tggcccggtg gcccgttcgt
     1321 ggcgctacct gaagagctac cgaattccgc atgcgcagcc gcagcaattt ccgggcgctc
     1381 tcgaaccgcc acagcggcca gtgcggatcc ggcccgggat ctacctttgc ggcgatcatc
     1441 gcgacaacgc ttcgattcat ggcgcgatga tcagcgggcg gcgtgccgcg gaagccgttc
     1501 ttgccgactt ggcgcgctaa gttgttctgt tgaatcggag tgagctagag gtctgaaggc
     1561 ttggccttgg accaaacgcc gagaaatcgc cgaatagcca gcgagatacc ggtgcaagcg
     1621 agaaaaactc cgccaagaga ggccgcgctg gcgatgcttt gaccgagaag gccgccggct
     1681 tcgccggtgt gcgcaaaacg catccatgcg cggatctttc taccgcgagt attctccgag
     1741 aatggttccc agcgaacttc gtcaccggtt tcgcgatcca gcgttaattg cgcgcgcttg
     1801 tcggggcgtc cgccatcgcc ggcgtcgatc tgaaaaatta cattcgcgga tttacctgcc
     1861 ggcaggcgca tggtgatgga ctgccattcc ggaactttcc tttccgcgag cgcgcacagg
     1921 gaagccaatc cttcgatttt gagtttttct tgcggccctg cccggactcc ggcgggtcga
     1981 ggcgctgcgt ttgcaagcgg cacggggctg ccgctcaagc gatacaccaa attgttggcc
     2041 cattgatagg acatcaccgt tgagcagacc acgattacga acagcggcag gcacatccag
     2101 aaaccgatcg cgttgtgcca gttgaaatcg cgagcgcggc cagagagtcc gcggcgaagc
     2161 gtcgtgccgg tctttagcgc gttccaggtc cacttgcgcg gccgccagag atagaggccg
     2221 ctgcacccaa ggaaaagaaa caacagattt gccgcgccgg tgatggcgcg agccgttggc
     2281 cgattagccc cactgacgcc gagccatcgg tgaatatttt cggtgtcatc aaagaacgat
     2341 cggagctgca cggcgcggtc ttccaggatt tgtccgttat aggggttcag gaaaacggta
     2401 cggctccggc cgtaggcaag ctctacggtt gcgttcggtt gggaacgcca tgtgaccgac
     2461 accgattgat cggattcgcg cgggagcgct tgcgcgagga gcgcatccat cgagaggagt
     2521 tgtgcgccgt cgctcgcggc gaccgttctt tcgtgccgtt gagcccaatg gacgatttgt
     2581 ttttcgaatc cgagggccgc gccggtcagg cacattgcaa ggatcacgct accggcgacg
     2641 atgcccgcag aaaggtgcag ccagaaaaat attttgcgaa tggtcatgag ctcggcggcg
     2701 cagcgatcag gcgtctgccc aacgcctcat caggttgtgg tagatgccgg tgagttggac
     2761 ggcgcttggg ctggcaggcg cgtcgcgatt cacgcgctgg atggcggcgt ccaggtcgaa
     2821 caataatgtg cgttcgccat catcgcgcac catgctctgg atccagaaga acgatgcgat
     2881 gcgggcgcca cgcgtcaccg gacgcacgtg atggagactc gtcgaggggt agaggaccat
     2941 gtggccggcg ggcagcttca cgctgtgcac cccatatgtg tcctcggcca ccagttcgcc
     3001 gccgtcgtat tcttctggct gcgcaagaaa taaagttgcg gacagatcgg tgcgaatgcg
     3061 atggcccgtt cccggcactt gacggatcgc gttgtccacg tgcgtgccaa aggattggcc
     3121 gccggtgtat ttgttgaaca gcggcggaaa aacgcgcaac ggtagcgcag cggaaatgaa
     3181 catggcgttt tgccccagag cggtaaggat cagctcgccc agctgctgag attccgcgct
     3241 tccttcgcgc aactgctggt tgtctttcac ttgagcggat tgatggccag cagtcacctt
     3301 gccgtccacc catagggcgg agtccagaat ttgccgcgct tgcgaaactt gcgctgcgga
     3361 aagcacatcc ggtattgcca gcaacatggt cgagcctttc ttgccagaag taaatttgcg
     3421 cgcgcttaaa acttgaagtt gagccctgcc aacgcggtga atcccgcgcc tggcaccagg
     3481 tgcgcaggat gaatttcgtt aatgtattcg cggttggcaa ggttgtagag attcagctgc
     3541 acgcccaggt gctcggtgac gggacggctg gccatcgcgt tgaataacca gtagctgggg
     3601 acttgcttca ccagtcccgt gaaaggatcg atgggcacag tggtgcttgc cgtgcgcgaa
     3661 tcgacgaact gcgcgccccc gccaaccgtg agcttccacg gcaaagcgtg ggtggtccac
     3721 aaattgaaag tgtttttcgg aacgttggcc agctgcgcgc cgacagcttg aggatagaat
     3781 tgcgagctca ccagcttgcc gtccaggtac gcatagctgc cgagcacttg ccatccatcc
     3841 gtaattttcc ccgtcacaga gacttgcgcg ccgttcactc gctgctcgcc ggccagcaca
     3901 ttgagcagag gattattggg atcgggctcg cgcgcgttca atttgtcggt gcgaaacacc
     3961 gcgccttcca cggacaaccg ctttccggga aaatcccact tcgtgccgag ctcaaacgtc
     4021 tggttatttt ccggaggcag attggcgttg ctggcgctta gggaaagcga ttccgccgag
     4081 gggttgaacg aggtgccgta atcgaaataa atgcttccat acggcttcgg tttgtagacc
     4141 acagccgcgc ggtagctcgg cttctggtcg acccggttga aggaggaaac cggcgcaacc
     4201 gactgactgt agtcggtatc gaagcggtcc cagcgcagcc cgccgatcac gctccactgc
     4261 cggcttaact tgaccgtatc aattgcgtac gcgccaaagc tgatcgccat cgctttgacg
     4321 tccgaagaaa ccgcggcggt gccgctcaat tcctggttct gatcgggatt cagcaaactc
     4381 gttcctggca ctccactcca ggcaaaccgc atcggcgaag acgtctcgcg gccgccctca
     4441 acacccgtta cgacggtgtg ctcggcaaag ccggttcgaa agcggaacgt gaggtccaac
     4501 tgatcgccga gataggtctc caagctccgc accgcgatct cgttgcgcgc taccgaaatg
     4561 tcttccagcg gcgtgctcgg gtccggtgtg cccgcgatgc gcggttccgt gatcagcacg
     4621 tcacgcttgt aatgtccgta ccgcacctgg ttccgtagcg tgacagctgt tccgaaatcg
     4681 tgctccccct tcgcagttgc gatgtccacg cccgttcgta ggtagttgcc gtcgcgaaat
     4741 ccgtagtagt tgctgcggtc cactggagac ggcccgttga acagccacgg aattccataa
     4801 tcgggcgtgt cgtcggcttg ttggtggtag tagctcagca caagccgtgt agtggtgccc
     4861 aagccgaacg ccagcgacgg cgccacgcca aaccgccggt tctccgcaat gtcccggtcc
     4921 gctacttgtg aatcgtgccc catcacattc agacggaatg cccccgtcgg tccgattggt
     4981 tcgttcacat ccgcggtaat acggcgcgtc aggtcgctgc caaattgcaa agtgccgccg
     5041 agaagctttg cgacctctgg cgccttggtt tcctgattca cgacgccgcc gctcgagccg
     5101 cggccaaacg tcatcgaact cggcccctgc agcacgtcca cgctttcgta gttgaacggg
     5161 tcgcggtaat aactgccgaa atcccgcatg ccatccagaa aaatgtcgtt gcgtcccgaa
     5221 aatccgcgga tcgtcaggtt gtctccctga gcgccgcctt ctcccgccgc tagactgatt
     5281 cccgccacat tgcgcagcgc atcccgaagt gtgttcacgc cttgctgctc caggatctgg
     5341 ttgtcaatca cgctgatcgt ttgtggcgtg tcgcgcagca gttcgctgta cttcgccgaa
     5401 gcgatcattt gcgcaccgtc agtcgaaact tccacggaag tcgccacctg cgccaattga
     5461 atggagacgg catccgctgc aacgaaccgg taactggcgc cgctatccgc gaccagcagc
     5521 ttcagcgcct gatccgatgt atacagaccc atcacgccct tcgagggcag tgtgcgcagt
     5581 tcgtcgtctg cgaacttcac cgagattccg caaactttgg cgaatgcatc catcgtgtcg
     5641 ccgagagtgc tggaggcaat ctcaaatcga cgtaccacca gagcctgcgg accaagcgga
     5701 gtgccgtctt tgcccctggc ttccgcgcaa ggagccgcac tagccaccct ggtgacgcca
     5761 agcgccgagt atgccaaaag cgttcccatg gcagcccacc gataagtcac cggccaatgg
     5821 tctacacgat tgattttaat ggacttactc cgatctggac gatccacgg
//
LOCUS       DASTPM010000240         2643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_584927, whole genome shotgun sequence.
ACCESSION   DASTPM010000240 DASTPM010000000
VERSION     DASTPM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584927"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1387
                     /locus_tag="VFR42_04250"
     CDS             236..1387
                     /locus_tag="VFR42_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEU0368395.1"
                     /translation="MLTSPAAFAAGVAILIVVVWDAFEVIVLPRRVTRRFRLSHFFYK
                     NLWSIWKSLARLIPSKKVRESAFGVFGPISLLILVAIWAVGLVYSFALMQYGAGSSVN
                     VSVPSRTTFWTDIYLSGTTFFTLGLGDVVPRSNFARALVVTEAGLGFGFLAGVIGYLP
                     FIYGSFSKREVSITLLDSRAGTPPSAGELLRRHSYPYGADSLRELLKEWELWSAELME
                     SHLSYPVLAYFRSQHDNQSWIASLTAILDTCALIMVGVEGACERQAELTFAISRHAVV
                     DLSQVFGTSPRLLPQDRLPAAELLRIRNVLAQHGLKLNDGAAADARLIELRDMYEPYI
                     FALASYLNQTLPPWIPQKKGKDNWQTTAWGQAAGLLEPQPATHHHDDHF"
     gene            1624..2589
                     /locus_tag="VFR42_04255"
     CDS             1624..2589
                     /locus_tag="VFR42_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HEU0368396.1"
                     /translation="MKAIRVHKFGGPEVLQLEEVPTPKPAAGQVLVKIRAAGVNPADT
                     YARTGNYAILPQLPYTPGTDGAGIVESIGDSVSKVRPGDRVYLAKNLTGSYAEYALAL
                     EPQVYRLPEKNSFAQGAGVFIPYGTAYHALHHQALAKGGETVLIHGASGGVGIAAVQI
                     ARAMGCTVFGTAGTPKGLSLIEREGAQLSFDHCKPDYKDQITKATAGKGVDVILEMLA
                     NVNLGADLKLLAQYGRVIVIGSRGDVTITPRDLMARRASVRAFTLWAITEAEANDIHA
                     GIFAGLDNGTFRPVVGAELPLRDAAQSHIDVMKSSGAHGKIVLIP"
BASE COUNT          499 a          968 c          616 g          560 t
ORIGIN      
        1 aaatccccgg cggcgacaag gccagattcg acgcggccgt caaggccgcc gaaaccggct
       61 gccccgtctc caaactcttc aaagccgaaa tcaccgttaa tgccaaactg gtcagctgag
      121 ttccggtcga gctagaacct accggttcgc gggcgtttta tgctcgctgg atctgctttt
      181 tctttcgtcg tacatgctca gtacaactta gccccaaaca ggtacaatct cgcccatgct
      241 gacatccccc gccgctttcg ccgccggcgt cgccatcctc atcgtggtag tctgggacgc
      301 cttcgaggtc atcgtccttc cgcgccgcgt cacgcgccga ttccgtctgt cgcacttttt
      361 ctataaaaat ctctggagca tctggaaatc gctcgcccga ctcatccctt ccaaaaaagt
      421 ccgcgaatcc gccttcggcg tcttcggtcc catctctttg ctgatcctgg tggccatctg
      481 ggccgttggc ctggtctaca gcttcgctct catgcagtac ggtgctggtt cctctgtcaa
      541 cgtttccgtc ccttcccgga ccactttctg gaccgacatc tatctcagcg gcaccacttt
      601 cttcactctc ggcctcggcg atgtcgtccc gcgcagcaac ttcgcgcgtg ccctcgtcgt
      661 caccgaagcc ggcctcggct ttggcttcct cgccggcgtc atcggctacc tgcccttcat
      721 ttacggttct ttttccaaac gcgaagttag tatcactctg ctcgactctc gcgctggcac
      781 tccgccatcc gccggagaac ttcttcgccg ccacagctat ccttacggcg ccgactctct
      841 ccgcgaactc cttaaagaat gggaactctg gtccgccgaa ttaatggaga gccatctcag
      901 ttatcccgtg ctggcctatt tccggtccca acacgacaat cagtcctgga tcgcgtctct
      961 caccgccatc ctcgacactt gtgcgctgat catggttgga gtcgaaggtg cctgcgaacg
     1021 acaggccgaa ctcaccttcg cgatctctcg tcacgccgtc gtcgacctct cgcaagtttt
     1081 cggcacttct ccacgtctgc tgccgcaaga ccgtcttccc gccgccgagc ttctccgcat
     1141 ccgtaacgtc ctcgcccaac acggcctcaa gctaaacgac ggcgcagccg ccgacgcgcg
     1201 ccttatcgaa cttcgcgaca tgtacgagcc ttacatcttc gccctggcct cgtatctcaa
     1261 ccaaacactt ccgccctgga tccctcagaa aaaaggcaaa gacaattggc aaaccaccgc
     1321 ctggggccaa gccgccggcc tcctcgaacc ccaacccgct acccaccatc acgacgacca
     1381 cttctagggc gccaacaccg tcagcctcac ccgacctagt cattccgagc gaagcagtcg
     1441 aggactcgct tgatcactgc cttcgcgcaa cagcgtcctt ggtagcggcg actttcaagc
     1501 ggcctccacc cgtcctccgc tctaccgtct ccgtgatctc tgtgccctgt gtgttaaaat
     1561 tcgcttggtt ttgtcactga ttttccattt tcaattttcc aatttctatc ttctggggta
     1621 accatgaaag ccattcgcgt ccacaaattt ggcggtcctg aagttctcca actcgaagaa
     1681 gtccccacgc ccaagcccgc cgccggacaa gtcctggtca agatccgcgc ggcaggcgtc
     1741 aatcccgccg acacttacgc gcgtaccggc aattacgcaa tccttccgca actcccttac
     1801 acgcccggca ccgatggcgc cggcatcgtc gaatccatcg gcgacagcgt cagcaaagtc
     1861 cgtcccggcg atcgcgtcta cctcgccaag aatctcaccg gctcttacgc cgaatacgcc
     1921 ctcgcgctcg aacctcaggt ctatcgcctg cccgagaaaa attctttcgc gcaaggcgcc
     1981 ggcgttttca ttccctacgg cactgcttac catgcgctcc atcaccaggc gctggccaaa
     2041 ggcggcgaaa ccgttctcat ccacggagcc agcggcggtg tcggcatcgc cgcggttcaa
     2101 atcgcccgcg ccatgggctg cactgtcttc ggcactgctg gcactcccaa gggcctgtcg
     2161 cttatcgaac gcgaaggcgc ccagctctcc ttcgatcact gcaagcccga ctacaaagac
     2221 cagatcacaa aggccactgc cggcaagggc gtggacgtca ttctcgaaat gctcgccaac
     2281 gtgaacctcg gcgccgatct caaactcctc gcccaatacg gccgcgtcat cgtcatcggc
     2341 agccgcggcg atgtcaccat caccccgcgc gacctcatgg cccgccgcgc ctccgtccgg
     2401 gccttcacgc tttgggcaat caccgaagcc gaagctaacg acatccacgc cggcatcttc
     2461 gccggcctcg acaacggcac cttccggccg gtggtcggcg ccgaactccc cctaagagac
     2521 gccgcgcaat cccacatcga cgtcatgaag tcctccggcg cccacggcaa aatcgtcctg
     2581 attccctaac cccgggaaag agactcgccc gcagtatcgt gttctcaatc ggcggtttca
     2641 aaa
//
LOCUS       DASTPM010000241         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_469761, whole genome shotgun sequence.
ACCESSION   DASTPM010000241 DASTPM010000000
VERSION     DASTPM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469761"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFR42_04260"
     CDS             complement(<1..272)
                     /locus_tag="VFR42_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368397.1"
                     /translation="MTAFAVTQKRLVGAATIFLLAFYPAFAQRLPTSPGLHRGQTLVY
                     QLELSGSRSTRAESRVIAPQSAPSVGLNALCLLQVHVEQVTASGLRL"
     gene            complement(269..1216)
                     /locus_tag="VFR42_04265"
     CDS             complement(269..1216)
                     /locus_tag="VFR42_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368398.1"
                     /translation="MTTSKSSARFCLLAVVCAIGTHGKPQAQGKPLGRVFTAESEERY
                     EVSVSMKAQTQSVSTETVASTTYVMPVVHSAQFQVTWNARRRILSTAPDGAAEIEETL
                     TPTHPCEQVAQPRETADARLLESLSSLCAALAAPVSIRYTEDAKGAQRESGVSKLPLD
                     FGEGQPPLLALWLRRAVRPSVVFPSLTFAVGASATQELRPSGELWNNAWGSESTDWLE
                     AESATPAASLHVVQQLAWDPPTQTKPATNVAKGTVPKHESFFADSITALSLLDGSVVR
                     ANRSASRTTTHKIEPVAGLPEPPDFSSRLTISVLIERLP"
     gene            complement(1230..3356)
                     /locus_tag="VFR42_04270"
     CDS             complement(1230..3356)
                     /locus_tag="VFR42_04270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0368399.1"
                     /translation="MKIHIRNRMLPSAATLLSFAVLFIASAAAAQQSFTLEQVLSAPF
                     PSSLITAKSVPRIAWVLDEQGKRNIYVAEAPDFKARRLTSYMDEDGQELSGLQFSSDA
                     NTIVFTRGDGKDKSGQSPNPASNPAGAEQAVYRIAWSGGETQKIDAGHAPQLSSKGVC
                     AYVKDGALWLATVVSTEKPVQLMIRGTNGEQRWSPDGSKLAFTSDRGDHKFIGIYDVL
                     TKGIRFVAPTVDSDSTPEWSEDGERLAFIRRPAIARDTPEGYFIEPDRPHPWAIWVAD
                     TDIWSAKELWHSGKELADSYTYMAGDTGGGVIHWAAENRIVFASEQDGWQHLYSIATS
                     GGTPTLLTPGNCEVEQWSFTPDGRSVLFNSNCNDIDRRHLWSVATSGGKLAQLSSGQG
                     IEWSPIAVADSGYLAFVASDASHAARPFVRRLAGSSSEQKPLAAETWPAQFPADKLIT
                     PQQVVFKSPDGLDIHGQLFLPRNIKPGEKRPGVLFFHGGSMRQMLLGWHYMYYYSNAY
                     GMNQYLASRGYVVLSVNYRSGIGYSRAFREAPGRAGRGASEYKDVVAAGKYLQSRPDV
                     DPSHIGLWGGSYGGYLTALGLARNSDLFAAGVDLHGVHDWPTDNWDGKNISPELTKIA
                     HESSPVSSVDKWKSPVLFIHGDDDRNVYFAQTVDLAARLRERGVHIEQLIFPDEIHDF
                     LLHHSWLKAYSAASDFFDRTLAKSAN"
     gene            complement(3366..>4399)
                     /locus_tag="VFR42_04275"
     CDS             complement(3366..>4399)
                     /locus_tag="VFR42_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU0368400.1"
                     /translation="LTRATLPVAGLNQPIQLENVRGEWRDTGRKFTLEKAGAFGATWS
                     GSVELARTVGDESGEGDVPHWSFQLQADQLDAAELDRWIGPRARPNWLQRLLPAGLGG
                     NSTPLSSLTPLRRIRAEGDLKVDEIAIEKIHLKGFRAHARLDGSDLNLENAQAQWSGG
                     TAQGRLSASLSGQPTYDVVASFDHISLVQTPWLADVANHLAGTASGRIEMKAKGIGRE
                     ALLKSLSGKGDLHLSRVELRGWDLAGTMAQGEWKTGISRWASGSGTFHIGDGGFELNT
                     LRLASSSEEFLLKGSISFSQDADLTAESHVVGRAVRPETAVRFLTISGPLTEPKVSLE
                     KVTAQQPGD"
BASE COUNT          936 a         1262 c         1285 g          916 t
ORIGIN      
        1 aggcgaagcc cggaggctgt cacttgctca acgtgtacct ggaggaggca taaagcgttc
       61 aggcccacgc tgggcgcgga ttgcggagca attacccggc tttcagctct agtggagcga
      121 gagccactta gttccagttg gtacaccaga gtttggccgc gatgcaggcc aggcgaggtc
      181 gggaggcgct gtgcaaatgc cggataaaag gccagcaaga agattgtggc ggcgcccaca
      241 aggcgcttct gtgtcacggc gaatgcggtc acggtaagcg ctcgatcaat acggatattg
      301 taagcctcga tgaaaagtcg ggtggttcgg gaaggccggc gacgggctct atcttatgag
      361 tcgtcgtacg agacgccgag cgattggcgc gcacaacgct accgtcgagc aaggaaagtg
      421 ccgtgattga gtcggcaaaa aaactttcgt gtttgggaac cgttccctta gccacattcg
      481 ttgccggctt tgtttgcgtt ggaggatccc aagccagttg ctgcaccacg tgcaacgagg
      541 cggcgggcgt ggcggattcg gcttcgagcc aatcggtcga ctcggaaccc caagcattgt
      601 tccataactc accggagggg cgtaattcct gtgtggcgct tgccccgact gcgaacgtca
      661 acgagggaaa aactacgctg ggccgcacgg cgcgccgcaa ccacaaggcc agaagcggcg
      721 gttggccctc tccgaaatcc agaggcaact tggaaacacc agactcgcgc tgcgcacctt
      781 tcgcatcctc cgtataacga atgctgacgg gcgcggctag agccgcgcag agactcgaca
      841 acgattccag caacctggcg tccgcagtct cgcgtggttg cgcgacctgt tcgcacggat
      901 gcgttggcgt caaagtttct tcgatttcag cggcaccatc aggcgcggtc gaaagaatcc
      961 ttcgcctggc gttccacgtc acctggaact gcgctgaatg tacgacgggc atgacgtaag
     1021 tcgtggaagc gacggtctcc gtggaaacac tctgggtttg agctttcatg ctgacggaaa
     1081 cctcgtagcg ctcttcgctt tctgccgtga aaacacgccc gagcggttta ccttgcgcct
     1141 gtggcttgcc atgtgtgcct atcgcacaga cgacggccag caggcagaat cttgcggagc
     1201 ttttgctggt ggtcattggg gcagcagagt caattagcgg atttcgccag agtccggtca
     1261 aagaaatcgc tcgccgcgct ataggctttg agccacgaat gatgcaacaa aaagtcgtga
     1321 atttcgtcgg ggaaaatcag ctgttcgatg tgcacgccgc gttcgcgtaa acgagccgcc
     1381 aagtcgaccg tttgcgcgaa gtaaacgttg cggtcatcgt cgccgtgaat gaaaagaacg
     1441 ggcgatttcc atttgtcgac ggaagagact ggagaggatt cgtgcgcgat tttggtgagc
     1501 tcgggcgaaa tatttttgcc gtcccagtta tccgtgggcc agtcgtgaac accatgcagg
     1561 tccactcccg cggcgaaaag atccgagttg cgggccaagc cgagtgctgt gagatagcca
     1621 ccgtagctgc cgccccacag gccgatgtgt gagggatcca catcggggcg ggactgcaga
     1681 tacttccctg ccgcgacgac atccttatat tcgctcgccc cgcggcctgc gcggccggga
     1741 gcttcacgaa aagcacggct gtaaccgatg ccgctgcgat aattcactga gagcactacg
     1801 tagccgcggc tagccaaata ctgattcatg ccgtacgcgt tcgagtagta gtacatgtaa
     1861 tgccagccca gcaacatttg gcgcatcgaa ccgccgtgaa aaaagaggac accgggacgc
     1921 ttttcgccgg gcttgatgtt cctcggcaga aagagctggc cgtgaatgtc gaggccatcc
     1981 ggcgacttga acacaacttg ctgcggcgta ataagcttgt ccgctggaaa ttgggctggc
     2041 caagtctcgg cggctaacgg tttttgttca gacgatgaac cggcaaggcg acgaacgaat
     2101 ggccgcgcag cgtgtgaagc atccgaagcg acgaacgcaa gataaccgga atcggccact
     2161 gcaatgggac tccattcaat cccttgccca ctgcttaatt gcgcaagctt accccccgaa
     2221 gttgccacac tccacagatg ccgacggtca atgtcgttgc agttggaatt gaacaaaacg
     2281 gacctgccgt cgggtgtaaa cgaccattgc tcgacttcac agttgccggg agtcaggagc
     2341 gtgggggttc cgccgctcgt cgcgatcgaa tacaaatgtt gccagccgtc ctgttcgctg
     2401 gcgaaaacga ttcgattttc ggcggcccaa tgaatgacac cgccgcccgt gtcgcccgcc
     2461 atgtaggtat aggaatccgc taactctttt ccgctgtgcc aaagttcctt tgcgctccag
     2521 atatcggtgt cggcaaccca aatcgcccag ggatgcggcc ggtcaggttc gataaaatat
     2581 ccttccggag tgtcacgtgc gatagccggt cgacgaatga aagctaagcg ctcgccatct
     2641 tcagaccatt ctggcgttga atcgctgtcc accgttggcg caacgaaccg aatacctttc
     2701 gtgagcacat cgtagatgcc gatgaacttg tggtcgccac ggtcggaggt gaaagcaagc
     2761 ttgctaccgt cgggcgacca gcgctgttcc ccattcgtac cgcgaatcat gagctgcacc
     2821 ggcttttcgg tagagaccac agtggcgagc cacaacgcgc catccttcac ataagcgcac
     2881 acgcctttgc tagatagttg cggcgcgtgg ccggcgtcaa tcttttgcgt ttcgccgccg
     2941 ctccacgcaa tacggtatac ggcttgttca gccccagccg gattgctggc cggattcggc
     3001 gattggccgg atttgtcctt cccgtcgccg cgcgtgaaaa caatcgtgtt cgcgtccgag
     3061 gaaaactgga gaccggaaag ttcctgtccg tcttcatcca tgtacgacgt cagacggcgc
     3121 gccttgaagt ctggggcttc cgcaacgtaa atattccgtt tgccctgttc gtccaaaacc
     3181 catgcgattc gcggaacgga ttttgctgtg atcagactgc tcgggaaggg agcgcttaat
     3241 acttgttcca gtgtgaatga ctgctgagca gcggccgcag acgcgatgaa caagactgca
     3301 aaagacaata gtgtagcggc gctaggcaac attcgattgc ggatgtgaat tttcatcacg
     3361 ttcctctagt ctcccggctg ttgtgcggtg actttttcca gagaaacctt gggctccgtt
     3421 aacgggcctg agatagtcag gaaacgaact gcagtttcgg ggcgcacggc gcgtccaaca
     3481 acgtggctct ccgcggtgag gtccgcgtct tgcgaaaagc ttatgctgcc cttcaaaagg
     3541 aactcttccg aggaagaggc aagacgcagc gtgtttaatt caaagccgcc gtcgccaata
     3601 tgaaatgtcc cggaacccga cgcccagcgg gagattccgg ttttccactc gccttgcgcc
     3661 atggtccctg ccaagtccca gccgcgcaat tcgactctgc tgagatgcaa gtcgcccttg
     3721 ccggacaggc ttttaagcaa tgcctcgcgc ccgatcccct ttgctttcat ttcaatgcgc
     3781 ccgctcgccg tgccagccaa atggtttgcg acgtcggcca gccagggggt ctggacgagc
     3841 gaaatgtggt caaaactggc aacgacatca tacgttggtt gacctgacag actcgcggac
     3901 aagcgtccct gcgccgtgcc gcccgaccat tgtgcttgcg cgttttccag attcagatcg
     3961 gatccgtcca atctcgcatg agcacggaag cccttcaggt ggatcttttc gatcgcaatt
     4021 tcgtccactt tcaagtcgcc ctcggcacgg atacgcctta agggcgtgag cgacgagagt
     4081 ggtgttgagt ttccgcccag ccccgcgggc agcaagcgct gcagccagtt tgggcgggcg
     4141 cgagggccga tccaacgatc caactccgca gcgtcgagtt gatcggcttg aagttgaaaa
     4201 gaccagtgag gaacgtcgcc ttcgccactc tcgtcgccaa cggtgcgcgc cagctcgaca
     4261 gaaccgctcc aggtcgcgcc aaacgcaccg gctttctcca aagtgaactt gcgacccgtg
     4321 tcccgccatt cgccccggac attttcgagc tgaataggtt gattgagtcc cgcgacggga
     4381 agagtcgcac gggtcaggt
//
LOCUS       DASTPM010000242         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1190757, whole genome shotgun sequence.
ACCESSION   DASTPM010000242 DASTPM010000000
VERSION     DASTPM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190757"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFR42_04280"
     CDS             <1..576
                     /locus_tag="VFR42_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0368401.1"
                     /translation="KGVLFLTAHIGAWELSSYAHALYGYPLHYMARPLDNAPLDALVN
                     RYRGLSGNAPIFKNESARAILKILKDAGTIGILADQNTMPQEGVFVDFFGTPACTTAG
                     MARVALHTGAAVVPGYAVWDAALKKYRLRFEPPLELVRTGDTERDIAENTQLFAKVTE
                     DIVRRYPEQWVWIHARWKTRPQGEPPLYDFL"
     gene            604..1617
                     /gene="lpxD"
                     /locus_tag="VFR42_04285"
     CDS             604..1617
                     /gene="lpxD"
                     /locus_tag="VFR42_04285"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU0368402.1"
                     /translation="MKKTVQELASAVGAQLEGDGAFELMGVAAPERASSSDLIFVDAA
                     KHLDRAAASAARCVVVSENISVPGKTLLRAKDAKVAFAKAAGLLREPSIIASGVHPTA
                     VIAPLAKIAPRASIGPYAVIGEDAHIGENTQIGAHCVIGAGCWIGANCRIHPRVTFYS
                     NIRVGNRVEIHSGAVLGADGFGYAYGEGRHWKFPQTGIIEVADDVEIGANATIDRGSL
                     NDTHIAEGVKLDNLVHVGHNVQIGTHTVVAAQTGISGSSVLGHHVVVGGQVGIADHCT
                     LEDGAIVGAQAGIPTGKTIRGGQVVWGTPARPLHKFKEQYAWFARLPELAARIKKLEA
                     KES"
     gene            1670..2347
                     /locus_tag="VFR42_04290"
     CDS             1670..2347
                     /locus_tag="VFR42_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368403.1"
                     /translation="MIVVGGHTRSIGKTQLVCDLIAALPQARWLAGKITQYGHGVCAQ
                     NGQDCDCAPTDHVVALDWESADRTLTHAGSDSARFLQAGADRAFWLRTKQGYLAEGMP
                     LLREALASIRAHAKDDGACDAVNVMIESNSLLQFVKPSLYVTVFDASREDFKNSARQM
                     LDRADVFVFRRGLDENEVSQPPWLQLPAQLLRQKPSLLQREGEPLPEPLFQLAKRILE
                     SPPAVTI"
     gene            2593..4617
                     /gene="shc"
                     /locus_tag="VFR42_04295"
     CDS             2593..4617
                     /gene="shc"
                     /locus_tag="VFR42_04295"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEU0368404.1"
                     /translation="MASFVGMGEEYSSTATAPALLREQDWAQQFPDSIERGAEHLLSL
                     QAKEGYWLGELEADTTLESDYIYYLFVLGKADPVRIEKLANYVRQRQLQDGGWSIYPG
                     GPSELNATCKAYFALKLAGDDPRAPHLIAARDTIHRLGGLEHANSYVRFYLALVGAVG
                     WELVPAIPPELMLLPNWFSINIYEMSSWTRGIVIPMAILSALRPDWRLPEHARVDELF
                     KDPSNKTVAFEWSAQLISWKNFFLALDRALKVYEKLPWKPLRQRAIREAKQWMLQHIE
                     RTDGLAAIYPAMMNSIFALMALGHRPDDPLTWREIKEFSRFEIEDADTIRLQPCVSPV
                     WDTCIAMVALEEAGVAPDHPALVQAAEWILSKQILGPGDWQVKNKDAEPGGWAFEFRN
                     DYYPDVDDTAFVLMALQRVKFPDEKRMEGALRRGIQWMLSMQNRDGGWAAFDRDNDRK
                     LLCNIPFADHNAMIDPSTADVTARVLECLGRFGWTAEHPAVRRAVKFLLRDQCKDGSW
                     FGRWGVNYVYGTSGVLRALETLSLNAQDYCKRAVNWLKAVQKPDGSFGESLRSYHVES
                     SKGQGNSTPSQTAWGLIGLLAAADVSDPAVSQAAESLVRQQNEDGSWDEDDFTGTGFP
                     CVFYLKYHLYRNSFPVYALARFYKQAQNAASAEFCSIKFRPDEFRMRSGF"
     gene            4625..>5227
                     /locus_tag="VFR42_04300"
     CDS             4625..>5227
                     /locus_tag="VFR42_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0368405.1"
                     /translation="MKFPLRLTVDLWRSRLSHLFASGDRDGTIFHLPSAVLPALHKNG
                     AKNVSGENGHSAAESVRAAAHTAAPVLWIGGSEPLEHSEVGRVAFALNGQGRNVFLHT
                     DGQRLRQRIHEFHPDPRLFLTVELAGREALHDRITGRPDAFRRVIEGIRAAKLSGFHV
                     CAHVTVNEKTEVCETGELFELLDRYDVDGYIVSSGGRTPLV"
BASE COUNT         1068 a         1485 c         1593 g         1081 t
ORIGIN      
        1 aagggcgtgc tgtttctcac ggcgcatatc ggagcgtggg agctgtcgtc gtacgcgcac
       61 gccttgtacg gatatccgct gcactacatg gcgcggccac tcgacaacgc gccgctggac
      121 gcgctggtga atcgataccg aggcctatcc ggcaacgcgc cgatttttaa gaacgagtcc
      181 gcgcgagcca tactgaaaat tctgaaagac gccggcacca tcgggatcct ggctgatcag
      241 aacaccatgc cgcaagaggg agtcttcgtg gacttcttcg gcacgccggc gtgcacgaca
      301 gcaggcatgg cgcgcgttgc actgcacact ggcgcggcgg tggttcccgg ttacgcggta
      361 tgggacgccg ccctgaagaa ataccgcctg cgtttcgagc ctccgctcga actggttcgc
      421 actggcgata ccgaacgcga cattgccgaa aatacgcagc tcttcgccaa ggtcaccgag
      481 gatattgttc gcagatatcc ggagcagtgg gtgtggattc acgcgcgctg gaaaacacga
      541 ccacagggcg aaccgccgct gtacgatttt ctgtaaccaa tgagaggttc agctggagga
      601 gcgatgaaga aaaccgtgca ggaactggcg tcggctgttg gcgcgcaact cgaaggtgac
      661 ggcgcgttcg agttgatggg agtcgccgcg ccggagcgcg cctcttcgag cgatctgatc
      721 tttgtggacg ccgccaaaca cctggatcgc gccgccgcct ccgcggcgcg ttgcgtagtc
      781 gtgtctgaaa atatttctgt gccgggcaag acgctattgc gcgccaagga cgcgaaagtc
      841 gcgttcgcca aagcggcggg gctgctgcgt gagccttcga ttatcgcaag tggcgttcat
      901 ccgacggcgg taattgcccc gctagccaaa atcgcgccgc gtgccagcat cggtccgtac
      961 gctgtgatcg gcgaagacgc tcacattggc gaaaacacgc aaattggcgc gcactgtgtc
     1021 atcggggctg gctgctggat cggagcgaat tgccgcatcc accctcgcgt tacgttctac
     1081 tcgaatattc gggttggtaa ccgcgtggaa attcattccg gagcggtgtt gggtgcagac
     1141 ggtttcggct acgcttatgg agaaggtcgc cattggaagt ttccgcaaac aggaatcatc
     1201 gaggttgccg acgacgttga aatcggtgcg aatgccacta ttgatcgcgg ttcattgaat
     1261 gatacgcata tcgcagaagg cgtgaagctc gataatctgg ttcacgtcgg gcacaacgtg
     1321 cagattggca cgcatacggt ggtggcggcg cagaccggta tttctggttc gagcgtgctg
     1381 ggccatcatg tggttgtggg tgggcaggtg ggcattgcgg atcactgtac tctggaggat
     1441 ggtgcgatcg tcggagcgca ggcgggtatt ccgacaggca agaccattcg cggtggccag
     1501 gtagtctggg gcacgccggc gcggccgttg cataaattca aggagcaata tgcatggttt
     1561 gcgcgactcc cggaattggc tgcacgcatc aaaaagttag aggcaaaaga aagctaagag
     1621 gccaaagttg gcgagttcta acgcaacacc gaagtcggcg ccttgtcgct tgatcgtagt
     1681 cggcggccac acccgcagca tcggcaaaac gcaactggtc tgcgacctga tcgcagcgct
     1741 gccgcaagca cgttggctcg cgggaaaaat tactcagtat ggccacgggg tctgcgcgca
     1801 gaacggccag gactgtgatt gcgccccgac cgatcatgtc gtagcactgg attgggaatc
     1861 ggccgaccgc accctcacac acgccggctc ggacagcgcg cgcttcctgc aggcgggggc
     1921 cgaccgcgcc ttctggctgc gcaccaagca aggctatctg gccgaaggta tgccgctgct
     1981 gcgcgaagcg ctggcgtcca tccgggcgca cgccaaagac gacggcgcat gcgatgcggt
     2041 caacgtcatg atcgaaagca attcgttgct gcaattcgtg aagccgtcct tgtacgtcac
     2101 cgtgttcgat gcgtcgcgcg aagactttaa aaattccgcg cgccagatgc tcgaccgtgc
     2161 ggacgtcttc gtttttcgtc gcggcctcga cgaaaatgaa gtttctcagc cgccctggtt
     2221 gcagctcccg gcgcagttgc tgcgccaaaa gccgtcgcta ctgcagcgcg aaggcgaacc
     2281 gttgcctgag ccgcttttcc agctggcgaa gcgcattctg gaatcaccgc cggcggtgac
     2341 tatctgactt ccgcggggcc gcttgcccgg taaaaccttg tagcgttttg ccgcagtatc
     2401 gaatccaaaa aacgggacat cttgggcgtt gacccgtacc atcgaatgaa gggatactta
     2461 gggtagtacg ggaaagactg caagccgtcc tgaccactct ggaagcataa gtaaaataaa
     2521 gggttagagg cggcaaggct agtgccggaa atccatccgg cagacttggg ttgattctgg
     2581 gggaaagctg agatggcctc attcgtagga atgggtgaag agtattcgtc caccgccacg
     2641 gctccggcac tgctgcgcga gcaggactgg gcgcaacaat tcccagattc catcgaacgc
     2701 ggcgcggagc atctgctttc gctgcaagcc aaggaaggct actggctggg tgaactggaa
     2761 gccgacacca cgctggaatc cgactacatt tactacctat tcgttctggg caaagccgat
     2821 ccggtgcgca tcgaaaagct cgctaactac gtgcgccaac ggcaactgca agatggcgga
     2881 tggagcatct atccaggtgg accgtccgag ctaaacgcca cctgcaaagc ctattttgcg
     2941 ctaaagctcg ctggggatga tccgcgcgcg cctcacctga tcgccgcgcg cgatacaatt
     3001 caccgacttg gcggcctcga gcacgctaat tcttatgtgc gcttttatct cgcgctggtc
     3061 ggtgcagtgg gttgggaact ggttcccgca attccaccgg agctgatgct cctgcccaac
     3121 tggttttcca tcaacatcta cgagatgtct tcctggacgc gaggcatcgt catccccatg
     3181 gcaattctca gcgctttgcg gccggactgg cgtctgcccg aacacgcccg cgtggatgag
     3241 ttgttcaagg acccgtccaa caagaccgtt gcgtttgagt ggagcgcgca gctcatctcc
     3301 tggaagaatt tctttctggc gctggaccgc gcgctgaaag tctacgaaaa gctgccttgg
     3361 aagccgctcc ggcaacgcgc catccgtgaa gccaagcagt ggatgctgca gcacatcgaa
     3421 cgtaccgatg ggctggcggc gatttatccg gcgatgatga attcgatttt tgcgctgatg
     3481 gcgctgggac acaggccgga cgatccccta acttggcgcg agatcaagga attctcgcgc
     3541 tttgaaattg aggatgcaga caccatccgc ctacaaccgt gcgtttcacc agtgtgggac
     3601 acctgtatcg ccatggtcgc gcttgaggaa gcaggcgtcg ctcccgacca tccggcatta
     3661 gttcaagccg ctgagtggat tttgtcgaag cagatcctcg gccccggcga ctggcaggtg
     3721 aaaaacaagg atgcggagcc cggcggctgg gcattcgaat tccgcaacga ctactatccc
     3781 gacgtggacg atacagcttt tgtgctcatg gcgttgcagc gagtgaagtt ccccgatgag
     3841 aagcggatgg aaggcgcgct gcgtcgcggc attcagtgga tgctgagcat gcagaatcgc
     3901 gacggtggct gggcggcgtt tgaccgcgac aatgaccgca aacttttgtg taatatcccg
     3961 ttcgcggatc acaacgcgat gatcgatcca tccaccgcgg atgtaacggc tcgcgtcctg
     4021 gagtgcctcg gccgctttgg atggaccgcg gagcatcccg ccgtgcggcg cgctgtgaaa
     4081 tttctgttgc gagatcagtg taaagacggc tcatggttcg gtcgctgggg cgtgaactac
     4141 gtttatggaa ccagtggcgt actacgcgct ctcgaaacgc tttcgcttaa tgcgcaagac
     4201 tactgcaaac gggctgtaaa ctggctgaag gcggtacaga agcccgacgg cagtttcggc
     4261 gaatcgttgc gtagttatca cgtcgaatcg tccaaagggc agggcaactc gactccatct
     4321 cagactgcct ggggcttgat tggcttgctg gctgcggccg acgtcagcga tcctgcggtt
     4381 tcccaagccg ccgaatccct ggtgcgccaa cagaacgaag acggctcctg ggatgaagat
     4441 gatttcaccg gcaccggctt tccttgcgtt ttctacctga agtatcacct ctaccgcaat
     4501 tcgtttcccg tatacgcgct ggcgcgtttt tacaagcagg cgcaaaatgc cgcgagtgcg
     4561 gaattctgct ccatcaaatt ccgcccggac gagttccgga tgcgcagcgg cttttgacac
     4621 gcgcatgaag ttccctttaa gactgactgt ggatctctgg cgtagtcgcc tgtcacatct
     4681 ttttgccagc ggcgatcgcg acggaacaat ttttcattta ccctccgcgg tgctgcccgc
     4741 gctgcacaaa aatggtgcga aaaatgtgtc cggcgaaaat ggccacagcg ccgcggaatc
     4801 cgtccgagcc gccgcgcaca cggccgcgcc agtactgtgg atcggcggta gcgaaccgct
     4861 cgaacactcc gaggtcggcc gcgtggcctt cgcattgaat ggccaggggc gcaacgtttt
     4921 tctgcacacc gacggccagc ggttgcgtca gcgcattcac gaatttcatc ccgatccccg
     4981 gctgtttttg actgtcgaac tcgctggccg ggaagcgttg catgatcgga tcactggccg
     5041 tcccgatgca ttccgccgcg ttatcgaggg aatccgcgcg gcaaaacttt ccggcttcca
     5101 tgtgtgtgct cacgtgaccg tgaatgagaa gaccgaagtt tgcgagacgg gcgagctttt
     5161 tgagcttttg gaccgctacg acgtggatgg ttatattgtg tcgtccggcg gccgcactcc
     5221 cttggtc
//
LOCUS       DASTPM010000243         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_412458, whole genome shotgun sequence.
ACCESSION   DASTPM010000243 DASTPM010000000
VERSION     DASTPM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412458"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1284)
                     /locus_tag="VFR42_04305"
     CDS             complement(166..1284)
                     /locus_tag="VFR42_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0368406.1"
                     /translation="MRNTPPRSGPQRLYLIPVLSKALDILELLQSENQPKSLEEIYQR
                     TNISKTTVYRILKTFTHRGYLAQSENGLYRLVTRPRKVRFGFGGESSEMPFSEAVRES
                     LAAAAASAGVDLLVLDNRYDAATAVRNAEEFVKQGVDLVIEFQIDQRIAPVIADKIHE
                     AGIPLVAVDIPHPHAMFFGVDNFRVGFEAGALLAQHAKNEWRGRVSWALGLDIQEAGP
                     LVQSRMTGAFAGIRSKLPHLPDSKYVLLNAEGLRGKSHRMMKEFLRRHSREPGILVAA
                     ATDTSGLGALQAVREAKKEKDVVIVGQDCIPEALDEMEVPGSPFIGSVSHEAGSYGPQ
                     LIQLGLAILNGQTLPPYHYVRHRLVTALRLAKRLRAAR"
     gene            1443..2720
                     /gene="rhaT"
                     /locus_tag="VFR42_04310"
     CDS             1443..2720
                     /gene="rhaT"
                     /locus_tag="VFR42_04310"
                     /inference="COORDINATES: protein motif:HMM:NF010024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HEU0368407.1"
                     /translation="MAPNPAIGVLFHWLGGLASGSFYVPFRGVKRWAWETYWLVGGFF
                     SWIIAPWTLALLMTSDLLLVLRESPGRNVFWAYAFGVLWGLGGLTFGLTMRYLGMSLG
                     MAVALGYTAAFGTLMPPIFRGQFASEVLGTRSGLTILTGVVICLLGIVVAGAAGLSKE
                     REMTDEQKRASIKEFSLKKGLLVATFSGVMSACFAYGLAAGDPIKEITLRHGTAVLWQ
                     GLPVLIVVLLGGFTTNFIWCVILNIRYGTGYQYFNSEIRSAIPERSAELILENVIDAP
                     SEEMATAAASIDKTAKLRTGADTIRAPMLSNYLFSALAGTTWYFQFFFYTMGETQMGR
                     FKFSSWTLHMASIIIFSTLWGIALKEWKGASRKTWLLVVLSLFVLIASTVVVGYGNYL
                     GAKPSAPQEPVTRASALSAPEMHVQAKRIIVAC"
     gene            complement(2910..>4050)
                     /locus_tag="VFR42_04315"
     CDS             complement(2910..>4050)
                     /locus_tag="VFR42_04315"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0368408.1"
                     /translation="STPNPGYGTGWSDAGVIIPWTSWMQTGDQKVIEENWGGMEKYLA
                     AIESANPDYLWKKNYGIPFADWLAPEGVTPVDLIATAYWAYDANLMKQMAHATGRTEA
                     EKKYGELFAKIKEAFNQAYVKPDGFVGGVPPPPVFASGTDTKLSDKPVETQTGYVLAL
                     QMNMLPEALRPVAAKRLVDRIAASGWRLGTGFLGTPYLLSSLTDTGHADVAYRLLLNK
                     EYPSWGYLVEHGATTMWERWNGDQKRDDPTMNSYNHYAYGAVGEWIYRYAAGIDAAPD
                     DPGFHTVLLHPNFDARLGSVDFSYDSSYGTIRSAWSVAGKKATWKLTIPPNATGRLPL
                     SPEELKMYQLDGKPLENTDKIRRAGSTSGVFEAPAGSYQFEVALP"
BASE COUNT          795 a         1344 c          987 g          924 t
ORIGIN      
        1 cgtcccagcg atcctgcaga aagcgaagcc gttcctgagt cttcatatcc ccggacttcc
       61 ctaagcgatg aacaggttct atgcgctgca catcgcgccc gccccccaac cttacgcaat
      121 cagtttcagc agtgcaatag attaattgtt tcaatagaga aacaactaac gcgcggcacg
      181 cagacgcttc gccaaccgca acgccgtcac tagtctgtgc ctcacgtagt gataaggcgg
      241 cagcgtttgc ccgttcagaa tcgccagccc cagctgaatc aattgcggcc cgtagctgcc
      301 cgcttcgtgc gacaccgatc cgatgaacgg gctgcccggc acttccattt catccagcgc
      361 ttccggaatg caatcctgcc cgacgatcac aacgtccttt tcttttttgg cttctcgcac
      421 agcttgcaaa gccccaagcc cactggtgtc ggtcgccgcc gccaccagaa ttcccggctc
      481 gcgtgaatgg cgtcgcagaa attccttcat catccgatgg cttttgccgc gcagcccctc
      541 cgcattcaac aacacatatt tgctgtccgg caggtgcggc aattttgatc gtataccggc
      601 aaatgctccg gtcatccggc tttgcaccag cggacccgct tcctgaatgt ccagtccaag
      661 cgcccagctg acgcgccctc gccactcgtt ttttgcgtgc tgcgctagca gcgctccggc
      721 ctcaaatccg acgcggaagt tatccactcc aaaaaacatc gcatgcggat gcggaatgtc
      781 caccgccacc aacggaatcc cggcttcatg aatcttgtcc gcgatcaccg gcgcaatgcg
      841 ttggtcaatc tggaattcga tgactaggtc cacaccttgc ttcacgaatt cttccgcgtt
      901 gcgcacagct gtcgccgcat cgtacctgtt gtcgagaacc agcaggtcca ctcccgctga
      961 agccgcggct gcggccagac tctcgcgcac cgcttcagaa aacggcatct ctgaggactc
     1021 gcctccaaaa ccaaaacgca cttttcttgg ccgcgtcacc agccgatata acccattttc
     1081 cgattgcgcc aggtaccctc ggtgcgtaaa cgtcttcagg attcggtata ccgtggtctt
     1141 cgaaatattc gtccgctggt aaatctcctc cagggacttc ggttggtttt ccgactgtag
     1201 tagctccagg atgtccagag cctttgacag caccggaatc agatacagtc gctgcggtcc
     1261 gcttctgggc ggcgtgttgc gcataaggtg ctcgataata gcacccgcgc tgtttcaata
     1321 gatacattgc atttgtattg acgcagcgcc gtggacgaac taaagtcccg cacggcgcgc
     1381 ggtggctaac gttggcctct gccgcgctgc ttccttctgg ggaacacgag aggaacactt
     1441 ccttggcgcc gaaccccgcg attggtgtgt tgttccactg gcttggcggc ctcgcctccg
     1501 gcagttttta cgtgcccttt cgcggcgtca agcgctgggc ctgggaaacc tattggctgg
     1561 ttggcggctt ctttagctgg atcatcgcgc cctggactct ggccctgctc atgacttcgg
     1621 acctgctcct ggttttgcgc gagtcccccg gccgcaatgt tttttgggct tacgccttcg
     1681 gcgtcctctg gggccttggt ggcctcacct ttggcctcac catgcgctat ctcggcatgt
     1741 cgctcggcat ggccgtcgct ctcggttaca ccgcagcgtt tggcacgctg atgcccccca
     1801 tttttcgcgg ccagttcgcc agcgaagtct tgggaacgcg ctccggcttg accatcctca
     1861 cgggagtcgt tatctgtttg ctcggcatcg tcgtcgccgg cgccgcgggc ctctccaaag
     1921 aacgcgaaat gaccgacgaa caaaaacgcg cctccatcaa ggaattcagc ctgaaaaagg
     1981 gcttgctcgt cgccactttt tccggcgtca tgagcgcttg tttcgcctac ggtctcgccg
     2041 ccggcgatcc tatcaaggaa atcacgcttc gccacggcac cgccgtcctg tggcaaggat
     2101 tgccggtcct gattgtcgtg cttctcggcg gcttcaccac caacttcatc tggtgcgtca
     2161 ttctcaacat tcgctatggc accggctacc aatatttcaa ctcggaaatt cgcagcgcca
     2221 ttcccgaaag atccgccgaa cttattctgg aaaacgtgat cgacgcgcca tccgaagaaa
     2281 tggccacggc ggcggcatca atagacaaga ccgcaaaact tcgcaccggc gccgacacca
     2341 ttcgcgctcc catgctctcc aactatctgt tttccgcgct cgccggcacc acctggtatt
     2401 tccagttttt cttctacacc atgggcgaaa cccagatggg ccgcttcaaa ttttccagct
     2461 ggaccctgca catggccagc atcatcatct tcagcaccct ctggggcatc gccctcaaag
     2521 agtggaaagg cgccagccgc aagacttggc tcctcgtcgt cctcagcctc ttcgttctga
     2581 tagcctcgac ggtagtcgtc ggctacggca actacctcgg cgccaagcca tccgccccac
     2641 aagaacccgt cacccgcgcc tctgctctaa gcgctcccga gatgcatgtc caagcgaaaa
     2701 gaatcatcgt ggcctgttga ctttggtggc acaagaatcg tcgctgctgc tagtcgccac
     2761 tcgattgccc tgactaagtt ccttaaaatg aatgcttacg gaaacacagt ccactaccta
     2821 aggcccttat attcaatccg tagaaaaaaa gacgggtggg aatcctgatc gtcgttccca
     2881 gactttgtaa gctttttgtt tacagctacc tacgggagag caacctcgaa ctggtagctc
     2941 ccggccggag cctcgaacac gcctgaagtg cttcccgccc gccgaatctt gtcggtattc
     3001 tccaacggct ttccgtccaa ttggtacatc ttcaattctt ccgggctcag cggcaatctg
     3061 cccgtcgcgt tcggcggaat cgtcagcttc cacgtcgcct tcttgccagc aaccgaccag
     3121 gctgaccgaa tcgttccgta gcttgaatca taggaaaagt ccacgctgcc gagccgagcg
     3181 tcaaaattgg ggtgcagcaa cacggtgtga aatcccggat cgtccggcgc cgcatcgatc
     3241 cctgccgcgt agcgatagat ccattctccc accgcaccat acgcgtaatg gttgtaggag
     3301 ttcatcgtcg gatcgtcacg cttctgatcg ccgttccagc gctcccacat cgtcgtcgct
     3361 ccgtgctcca ccagataacc ccacgacgga tattccttgt taagcagcaa ccggtatgcc
     3421 acgtccgcat gaccggtatc cgtcaacgaa ctcagcaggt acggggtgcc cagaaatccc
     3481 gttcccagcc gccatccgct cgcggcaatc cggtccacca aacgcttcgc ggcgaccggc
     3541 cgcaacgcct ctggcagcat attcatctgc agagccaaga catatcccgt ctgcgtttcc
     3601 accggcttgt cgctcaactt cgtgtccgtc cccgacgcaa acaccggagg gggtggcacg
     3661 ccgcccacga acccatccgg cttcacatac gcctgattga acgcttcttt gatttttgca
     3721 aacagctcgc cgtacttttt ctccgcttcc gtccgccccg tagcatgcgc catctgcttc
     3781 atcaaattcg cgtcgtacgc ccaatacgct gtcgcaatca aatccacagg cgttacccct
     3841 tccggcgcca gccagtccgc gaatggaatt ccataatttt tcttccacaa gtaatccggg
     3901 tttgcagact cgattgcggc cagatacttt tccatgccgc cccaattttc ctcgatcacc
     3961 ttttggtcgc cggtttgcat ccacgaagtc catggaatga tcacgccggc atcgctccaa
     4021 ccggtgccgt agccgggatt cggcgtcgaa
//
LOCUS       DASTPM010000244         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_94920, whole genome shotgun sequence.
ACCESSION   DASTPM010000244 DASTPM010000000
VERSION     DASTPM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94920"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFR42_04320"
     CDS             <1..522
                     /locus_tag="VFR42_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU0368409.1"
                     /translation="LCGSRAESAARKDADMLARVAAQLKCAPQDLDASVERLLQEREV
                     ATRNLRSLLPKLADADVATRLQSIAPSGDGEVVVVEMLQNLGPEYLQFYATALTRSEK
                     VLALLAVQETGALLFAQHPAVGKDMNALLRGVFDQLGGKGGGTKDFARGALADQAKVS
                     AALALAESMLKQK"
     gene            complement(537..1307)
                     /locus_tag="VFR42_04325"
     CDS             complement(537..1307)
                     /locus_tag="VFR42_04325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEU0368410.1"
                     /translation="MPSSTALWHGPVSPLAQSAAYEVSLATTLEEVCACQRLRYQVFN
                     GELGEGLESSHALGLDRDHFDLICDHLMVRETKTGEVVGTYRMQTGYRAKGNAGYYSE
                     EFFDFAPYQAMRSAMLELGRASVQRDHRDSMVLMLLWKGIFRYACETGARYLVGCSSL
                     TSQDPAEGHALYSRLREEHLAPGYLQTVPRPDHVLPEMQTPPGPVKTPRLLRTYLELS
                     AKICGPPAIDREFRTIDFLTLLDLEEIPARLRARLTAD"
     gene            1449..1904
                     /locus_tag="VFR42_04330"
     CDS             1449..1904
                     /locus_tag="VFR42_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU0368411.1"
                     /translation="MLAGSSFTHLCRARDLLREVHDQPVSIRCVAREAAMSPFHFIRQ
                     FAAIFGETPHQFRIRARLERAKHLLALSDYSVTDVCMEVGFSSLGSFSTLFAHRFGSA
                     PSEYRRQLRSLMTVPGILPAVLEPGCLSLMARAFAILEKHPRPVLAPSE"
     gene            1985..2374
                     /locus_tag="VFR42_04335"
     CDS             1985..2374
                     /locus_tag="VFR42_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0368412.1"
                     /translation="MRIKLTSIMVDDQDKAEKFYTGVFGFTKKHDIPVGGGLRWLTVV
                     SPEGPGDLELSLEPNSNPAGKAFQEAMFKQRIPLAAFEVGDLRKEFDSLKKAGVVFTQ
                     DPTPTGPVTIAVCSDTCGNLIQLYQPA"
     gene            2429..>2965
                     /locus_tag="VFR42_04340"
     CDS             2429..>2965
                     /locus_tag="VFR42_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEU0368413.1"
                     /translation="MSPQADLRDYLASERTFLAWIRTGLALMGFGFVVARFGLFLQQI
                     QLVNHALAAQPTGLSLWFGTALIAAGILVNVGSAWRHVQLVRQLDRGDTSSRSLSYAV
                     ATALFLALVGLAMAVYLISVSDSADLRSSMSGETIMALEKENGIVVLPSNHSVNETVE
                     KLKGILQAKGVTIFALVDH"
BASE COUNT          601 a          894 c          822 g          648 t
ORIGIN      
        1 ctctgcggca gccgtgccga atccgccgcc cgcaaagatg ccgacatgct cgctcgcgtc
       61 gctgcccaac tcaaatgtgc cccgcaagac ctcgacgctt ccgtcgaacg tctgctgcaa
      121 gagcgggagg tggccaccag gaatttaaga agccttcttc caaaactggc agacgccgac
      181 gtcgcgacac gcctgcaatc gatcgcacca tcaggcgacg gcgaagtggt tgtcgtggaa
      241 atgttacaaa accttggccc ggaatatctc cagttttatg ccaccgctct cacgcgctcc
      301 gaaaaagttc tcgcgctcct ggccgttcaa gagaccggcg cgcttctctt tgcgcagcac
      361 cctgctgttg gtaaggacat gaacgccttg ctgcgcggcg tctttgatca gttgggcgga
      421 aaaggtggag gtacaaagga tttcgcgcgc ggcgcgctcg ccgaccaggc aaaggtttcg
      481 gccgctctgg ctcttgccga atccatgctg aaacagaagt aactcctttc cgcaacctaa
      541 tccgccgtta accgtgcccg caagcgcgct ggaatctctt cgaggtccag cagcgtcaag
      601 aaatcgatcg ttcggaactc ccggtcaatg gcgggcggtc cgcaaatctt cgcagagagt
      661 tcgagatagg tccgcaacag ccgaggcgtt ttgaccggac cgggcggcgt ttgcatctcc
      721 ggcagcacat gatccggccg aggaacggtt tgtaggtacc ctggggccaa atgctcctcg
      781 cgcaaccgcg agtaaagggc gtgaccttcc gcgggatcct gcgacgtcag cgaactgcat
      841 cccaccaggt accttgcgcc ggtttcacac gcgtagcgga agatgccttt ccacaacagc
      901 attaatacca tcgaatcgcg atgatcgcgt tggacagagg cgcgccccag ttccagcatc
      961 gcgcttcgca tggcttggta aggcgcgaaa tcaaaaaact cttcgctgta gtagccggca
     1021 ttgcccttgg cacgataacc cgtctgcatg cggtaggttc ccacgacttc accggtcttc
     1081 gtttcgcgca ccatcaagtg atcgcaaata aggtcaaagt ggtcgcgatc gagccccagc
     1141 gcatgcgacg attccagtcc ttcccccagt tcgccgttaa atacttgata gcgcagccgc
     1201 tggcacgcgc acacctcttc gagagttgtt gcgagcgata cttcatatgc ggctgactgg
     1261 gccaagggag aaacgggacc gtgccaaagc gcggtgctgg atggcatggg ccatcttcca
     1321 ccgacctgta tgaatgtttt gtcgaagcag cgttaaagtt cgcggacggt gcggttacag
     1381 ataagacctg agtccagcaa gcggccttgg caggttgaac ctttacgcgg gagaggtaga
     1441 atttgtttat gctggcgggc agcagcttta cgcatttgtg ccgggcgcgc gatctcttgc
     1501 gcgaagttca cgaccagccc gtctcaattc ggtgtgtcgc tcgtgaagcc gccatgtcac
     1561 cctttcactt cattcgccag tttgctgcga tcttcggcga aacgccgcac cagttccgca
     1621 ttcgcgcgcg cctagagcgg gcaaaacact tgctggcact cagcgactac tcggtcaccg
     1681 acgtctgcat ggaggtcggc ttcagcagtc tcggcagctt cagcactctc tttgcccacc
     1741 gcttcggctc ggcgccttcg gaatatcgcc gccaacttcg ctcccttatg acggtgccgg
     1801 gaatccttcc ggcagtgttg gagcctggtt gtttatcatt gatggccagg gctttcgcaa
     1861 ttttggagaa gcacccacgg ccagttctgg caccatccga gtagcattcg gtccggccgc
     1921 cgcaggcagg ccgtacgccg ctgcccgttt cggctaagcc atcaatttct ccctcgagga
     1981 actcatgcga atcaagctca ccagcatcat ggtagatgat caggacaaag cggagaagtt
     2041 ctacaccggt gtcttcggtt tcacaaagaa gcacgacatc ccggtaggcg gcggcttgcg
     2101 ttggctcacc gtcgtttctc cagagggccc cggcgatctt gaactttccc tggagcccaa
     2161 ctccaatccc gccgggaaag ccttccaaga agccatgttc aaacaaagaa ttcctctggc
     2221 ggcgttcgaa gtgggcgatc tccgcaaaga gttcgatagt ttgaagaaag ctggtgtggt
     2281 gtttacccag gaccctaccc caaccgggcc ggtgacgatc gccgtttgtt cagacacctg
     2341 cggcaacctc atccagcttt atcaaccggc ataggctcgg gcaaattcgc atcgctgatc
     2401 atctggaaag acggcgtata atttcgccgt gtcaccgcaa gccgatctgc gcgactatct
     2461 ggcctccgaa cgcactttcc tcgcgtggat ccgtaccggc ttggcgctca tgggatttgg
     2521 ttttgtggtg gcccgcttcg ggctgttcct gcaacagatt cagctcgtca accacgctct
     2581 agccgcgcag cctaccggtc tttccctgtg gttcggcacg gcattgatcg ccgccggcat
     2641 cctggtcaat gtcggttccg cctggcgtca tgtccaactc gtccgccagc tggatcgagg
     2701 cgacacgagc tcccgttcgc tctcctatgc tgtggcaacc gcattgtttc tggcattggt
     2761 cggcctggct atggccgtgt atctgatttc tgtaagcgat tccgcagatc tgcgttcgtc
     2821 aatgagtggg gaaaccatca tggctctgga aaaagaaaat ggcatagtcg ttctgccgag
     2881 caatcactcg gtcaacgaaa cagtcgaaaa gttgaagggc attctgcaag ccaaaggcgt
     2941 gacaattttt gcgctggtag accac
//
LOCUS       DASTPM010000245         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1277116, whole genome shotgun sequence.
ACCESSION   DASTPM010000245 DASTPM010000000
VERSION     DASTPM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277116"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFR42_04345"
     CDS             <1..335
                     /locus_tag="VFR42_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368414.1"
                     /translation="TPSDDPLVVSTIYEAPRRGLRAPLYAAPAAEGELNSDGMFSPDS
                     KGAFGRPDMKVYTPVSEGSHGTVLLSAKKQMRLESGTRLLLVVQPPPNGDPDAAQNID
                     LDDISPNP"
     gene            387..1145
                     /locus_tag="VFR42_04350"
     CDS             387..1145
                     /locus_tag="VFR42_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368415.1"
                     /translation="MKLLAAAFVVAGLCAASSVAQTPTPSENTSSDASLSGGTVVNAA
                     LNSSIDSKKAKPGDQIVAHTTEAVKSSDGRTILPNGTKLIGHVTKASARSNGQGEALL
                     ALQFEKAVLKDKQEMPINVVIQAVAAPAQAASTFSSDTERTTSPSSAGTPSSNPSMSG
                     SRGARQDGATPAPQQYPSASANGSSTGDANSAGPLPANTHGVYGLQGVQMSPNASGEG
                     TVLTSTGKNVHLDGGTRLLLAVQPQSASAMPGGK"
     gene            1248..>2253
                     /locus_tag="VFR42_04355"
     CDS             1248..>2253
                     /locus_tag="VFR42_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU0368416.1"
                     /translation="MASSQATPKLPGAGPAKKLFLVDAMAHVYRAFFAPMNRMNAPSG
                     IPTKVPFLFNNILRRLLNDYKPDYVGIVFDTRQPTFRDKLFEQYKAKRAPMPDELAQQ
                     LPYVRRLCEAMRLPILELDGYEADDIIGCLAQKAAKQNLDVLLVTNDKDMMQLVDGNV
                     RVLRAGTGGAKNDVIVDAEKVKEILGVPPEKVVDVMSLMGDTIDNIPGAKGIGEKGAT
                     ALIQQYGSVENALEHAAEVSNKRYREALQQQREQVLMSKQLATLATTVPVELDLPSLA
                     RREPDVPALSALYRELGFNSLLRELGSEAVAASAVANAEPVMQTDYAQLGSPGEFGDY
                     L"
BASE COUNT          493 a          606 c          743 g          411 t
ORIGIN      
        1 cgacgcccag cgacgatccg ctggtggttt ccacaattta tgaagcgccg cgacgcgggc
       61 tgcgcgcgcc gctgtatgcc gcgcctgccg cggaaggcga gttgaactcc gacgggatgt
      121 tttcgccgga cagcaaaggg gctttcgggc gcccggacat gaaggtttat acgccggtgt
      181 cggaaggttc gcacggcacg gtgttgctct cggccaagaa gcagatgcgg ctggagagcg
      241 gcacgcgttt gttgttggtg gtgcagccgc cgccgaatgg cgatcccgat gcggcgcaga
      301 atattgattt agacgatatt tctcccaacc cgtagtccag ccaattccag cctccatcaa
      361 ttccaaaccg gaggaggttg tttcccatga aactgttggc agcagctttt gttgttgcgg
      421 ggctttgcgc ggccagttcg gttgcacaga cccctacgcc atcggaaaac accagcagcg
      481 acgcttcact atcgggcgga acggtggtga atgccgcgct gaattcgtcg atcgattcga
      541 aaaaagcgaa gccgggcgat caaatcgtcg cgcacaccac cgaagcggtg aaatcctcgg
      601 acggcagaac gattttgcca aatggaacca agctgattgg acatgtgacc aaggcttcgg
      661 cgcgttcgaa cgggcagggc gaagcgctgc tggcgctgca gttcgagaag gcggtgctga
      721 aagacaagca ggaaatgcca atcaatgtgg tgatacaggc ggtggcagcg cctgcgcagg
      781 cggcgtcgac tttctcttcc gatacggaac ggaccaccag cccaagctcg gcgggcacgc
      841 ctagcagcaa tcccagcatg tccgggagcc gcggagcgcg gcaggacggc gccacgcctg
      901 caccgcagca gtatccgagt gctagtgcaa acggaagttc tactggtgac gcaaattccg
      961 cagggccgct tcccgcgaat acgcacggcg tgtatggctt gcagggcgtg caaatgagcc
     1021 caaacgcgag tggcgaagga acggtgctga cttccaccgg gaagaatgtg caccttgacg
     1081 gagggacgcg attgctgctg gcggtgcagc cgcaaagcgc aagcgcaatg cctggaggca
     1141 agtaagggac gagacaaaaa aaacggtcgg tccaaggggc gcgaatgtgc gccccttctt
     1201 ttttgggtgc cgaagacctg cagcggcgct ttgctatact cggctggatg gcttcttcgc
     1261 aagcgactcc caagctgccg ggcgcgggtc ccgccaaaaa attgttcctg gtggacgcca
     1321 tggcgcatgt ttaccgggcg ttttttgcgc cgatgaaccg catgaacgcg ccttccggga
     1381 ttccgaccaa ggtccccttt ttgttcaaca acatcctgcg gcggctgctt aacgactata
     1441 agccggacta tgtgggaatc gtttttgata cgcggcaacc gacatttcgc gacaaattat
     1501 tcgagcaata caaggcgaaa cgcgcgccga tgccggacga gctggcgcag cagttgccgt
     1561 atgtgcggcg gctgtgcgag gcgatgcggc tgcccattct agagctggat ggctacgagg
     1621 cggatgacat tatcggatgc ctggcgcaga aagccgcgaa acagaacctg gacgtgctgc
     1681 tggtcaccaa cgacaaggac atgatgcagt tggtggacgg aaatgtgcgc gtgctgcggg
     1741 ccggaaccgg cggcgcgaaa aacgatgtga tcgtcgacgc ggaaaaagtg aaggagattc
     1801 tgggagtacc gccagagaaa gttgtggacg tgatgtcgct gatgggcgac acgatcgaca
     1861 acatacctgg agcgaaaggg atcggcgaaa aaggcgcgac ggccctgatc cagcaatacg
     1921 gcagcgtgga aaatgcgctg gagcatgctg cggaggtctc gaataaacgg tatcgcgagg
     1981 cgctacagca gcagcgcgag caagtgctaa tgtcaaagca actggctacg ctggcgacta
     2041 ctgtgccggt ggaactggat ttgccgagcc tggcgaggcg cgagccggat gttccggcgc
     2101 tttccgcgct gtatcgcgag ctgggattca attcgctgct gcgggaactg ggttcggagg
     2161 ccgtggcggc ttcggcggtg gcaaacgcag agccggtgat gcagaccgat tacgcgcagt
     2221 taggttcgcc gggcgaattt ggcgactatc taa
//
LOCUS       DASTPM010000246         5818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1104537, whole genome shotgun sequence.
ACCESSION   DASTPM010000246 DASTPM010000000
VERSION     DASTPM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104537"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFR42_04360"
     CDS             <1..799
                     /locus_tag="VFR42_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0368417.1"
                     /translation="MARAHRQIPLLISRRVGFPLGSGYFSRARFSAATRIIANSNWVA
                     EQTAASGAPKEKLVVIYEGVEIPPLPSATGRRAARMRWGIADQDRLLGCAGVLLEDKG
                     QEWVIRALAQLRQEFPRLKLLLAGEGPDRARLQKLAAELSVQDAVLFAGFVKDVESFY
                     RAIDVFVFPALFEGLGTSLLAAMAHGVPSVTYFGCALGEIVENGKSGLQVEPRNAPAL
                     GEALRKILRDDNLATQLGAAGRRRIENIFAADRMVEETLRFYREVCA"
     gene            884..2527
                     /locus_tag="VFR42_04365"
     CDS             884..2527
                     /locus_tag="VFR42_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368418.1"
                     /translation="MSTLVRLFLLFFALSELVCAQHTAPGGKTVDLSLTAVGRQHHPI
                     HTNSKEAQEYFDQGITLVYGFNHEEAARAFESASHLDPASPMPLWGIALAVGPNYNLD
                     VDAEREKQAFEAIQKAAKLAQHAPQVEQDYVNALAARYSGDANPDYKRLARNYADHMR
                     ALTKKYPDDLDAATLYAESLMNLNPWKLWSLDGAPGEDTAEIVSVLESVLARDPLHAG
                     ANHYYIHAVEASPSPQRALPSAARLEEMVPKAGHLVHMPSHIYSRVGYFSNAAESNAK
                     AIAADATYAKEAERSGSLYDLMYHSHNEHFLAFAAAMEGRYTEARRAADAMEKRLLPH
                     ADTMPMLESFLWTPIWVDLRFAKWQEILARPEPPANRKVPHLMWRYSRTIAYAAERQA
                     AKAEAEHALYAKEAGLLPHEIPLGQMNPNESMLAVMNEIVAAKLHAAKGNAESALKHW
                     RTAVEAQDKLNYTEPPDWYYPVRESLGASLLAGGNAKEAEQDFREDLRRNPRNPRSLF
                     GLKESLRAQHLENDAAWVDREFQRTWKHADSQLKIGDLY"
     gene            complement(2662..2985)
                     /locus_tag="VFR42_04370"
     CDS             complement(2662..2985)
                     /locus_tag="VFR42_04370"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0368419.1"
                     /translation="MNSQRGQAFPFHRNERRIPMEIGVYLEGNRQVPGAESTFTENVS
                     ERGARVISVRRWEPNDRLTVTSRSGEFRSSARVAYCQPMQGDGFAIGVEFLEPRGRWV
                     VQNAS"
     gene            complement(2999..4318)
                     /locus_tag="VFR42_04375"
     CDS             complement(2999..4318)
                     /locus_tag="VFR42_04375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU0368420.1"
                     /translation="MRLWSRIAEFLRRWPSAAWTLGFLGVGLATFPLIVYFRSANFTG
                     SYQTYLYLEVGGTLLSFCYAANAVVRFRGTHDRIALILSFGFVLSGLIETVGYFHFGQ
                     YLHAGSSAFLHIPLGWMVSRTLLAVLLLAALAVETYTPTSRQPSKEIAAALLVVALCA
                     YATSAAFLAAPSAPSSHPARLLSRSWDLLPAALFFVAAMAFRWRLSKDAKSASVYDYS
                     LFIVASLNVFCHVSATFSRHLLDGPFFLAELAKTTSYVVMLLGALLDQARLFDQVRTL
                     AVSDPLTGLANYRRLLSVIEAELDRSRRTQRPFSVVLFDMDGLKAINDRYGHLTGSRA
                     LVRLGKILRNHCRAIDTAARYGGDEFAIVFPEAGSDIASRIVGRIHEKMAAEVEMPGL
                     SASAGTATFPDDGDTPEKLLAAADRALYRMKHRGSSIQNLSRIAACL"
     gene            complement(4624..4857)
                     /locus_tag="VFR42_04380"
     CDS             complement(4624..4857)
                     /locus_tag="VFR42_04380"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEU0368421.1"
                     /translation="MPATKVTVNNNGSIRIEGDFEIVDPQGQAFGLAGRTAIGLCRCG
                     HSANKPFCDGSHKTSGFSDTVVARELPAAKPKI"
     gene            complement(4876..5376)
                     /locus_tag="VFR42_04385"
     CDS             complement(4876..5376)
                     /locus_tag="VFR42_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU0368422.1"
                     /translation="MSVFDEMLAHNASYAQRFDLGHLQTPPVRKLVILTCMDSRMDLE
                     QLLGLRVGDAHMIRNAGGLATDDAVRSLILSTHLLGTRSIAIIQHTECGLMSITDEEF
                     RWRLSTSTGKDASHLQFHAFHDLDQNVREQVARVRRDPFLPKQIEVRGFVYDVQRGSL
                     REIKSE"
     gene            complement(5409..>5818)
                     /locus_tag="VFR42_04390"
     CDS             complement(5409..>5818)
                     /locus_tag="VFR42_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0368423.1"
                     /translation="MCGKNRTIHELIDYYEFCGVRGEEAPTPDLQVPELTPRELKARL
                     DRGDDLYILDVREPHEYQICNIKGHLLPLGELPRRLHELDSSREIVAHCRSGKRSAEA
                     VDFLRKAGFRKIHNLKGGILAWSDEVDPSVPKY"
BASE COUNT         1277 a         1709 c         1708 g         1124 t
ORIGIN      
        1 gatggccaga gctcaccggc aaattccgct gctaatttca aggcgggttg gattcccgct
       61 cggttcgggc tatttttcgc gggcgcgatt tagcgcggcc acccggatta ttgccaattc
      121 gaattgggtc gcggaacaaa ccgcggcctc cggcgctccc aaggaaaaac tggttgttat
      181 ttatgaaggt gtggagattc cgcccctgcc gagcgctact ggaaggcgcg cagcccggat
      241 gcgttggggc atcgccgacc aggaccggct gctcggttgt gcgggagtac tgctcgaaga
      301 caagggccag gagtgggtga tccgggcgct ggcgcaattg cgccaggaat ttccgaggtt
      361 gaaactgctg ttggcgggcg aggggcccga ccgcgcaagg ctgcaaaagc tcgccgccga
      421 gcttagcgta caggatgcgg tgctctttgc gggtttcgtg aaagatgtgg aatcgttcta
      481 tcgcgcgatc gacgtgttcg tattcccggc gttgttcgag gggctgggca cttccttgct
      541 cgcggccatg gcccacggtg tcccatccgt gacctacttc ggctgcgctt taggcgagat
      601 tgtagaaaat ggcaagagcg gcttgcaggt cgagccacga aacgccccgg cattgggcga
      661 agcgctgcgc aaaatcctgc gggacgataa cttggcgacc caattgggcg cggctggtcg
      721 ccgcagaatc gaaaacattt ttgccgcaga ccgcatggta gaggaaacgc tgcgctttta
      781 tcgcgaagtt tgcgcctagc tgccaagggg ccactcctcg aaaaaccttg cctcggcatt
      841 tttccgctgg taagttgagc tgcaaccgtc gacggagaaa tccatgtcaa cgctggttcg
      901 cctcttcttg ctcttttttg cgcttagcga attggtttgc gcgcaacata ctgcccccgg
      961 gggaaaaacc gtcgacttga gcctgacggc tgtcggtcgt cagcatcatc cgattcatac
     1021 caacagcaag gaagctcagg aatatttcga ccagggcatc acactcgttt atgggttcaa
     1081 ccacgaggag gcggcacgcg catttgaaag cgccagccac ctcgatccgg catcgccgat
     1141 gccgctgtgg ggaatcgccc tggcagtcgg gccaaactac aacctggatg tggacgccga
     1201 acgggaaaag caggcgttcg aggcaataca gaaagccgcg aagctcgcgc agcacgctcc
     1261 acaggtggag caggactacg tgaacgcgct ggccgcgcgc tactccggcg acgccaatcc
     1321 cgattacaaa agactggcac gaaactatgc cgaccacatg cgagcgctga ccaaaaaata
     1381 tccggacgat ctcgacgctg cgactttgta cgccgagagc ctgatgaatc tgaatccctg
     1441 gaagctctgg agcctggacg gtgcgcccgg agaagacacc gccgaaatcg tcagcgtgtt
     1501 ggagagtgtc ttggcgcgcg atccgctgca cgccggcgca aaccactatt acattcatgc
     1561 ggtcgaagct tcgccctcac cgcagcgtgc tctgccaagt gcggcacgcc tcgaagagat
     1621 ggtgccgaaa gccggacacc tcgtacacat gccgtcgcac atttattcgc gcgtcggata
     1681 tttttcaaac gcggcggaga gcaatgccaa agcgatcgcc gcggacgcca cttatgcaaa
     1741 agaagccgaa cgcagcggca gcctgtacga cctgatgtac cacagccaca acgaacactt
     1801 tctggctttt gccgcggcaa tggagggccg ctacacagag gccagacgag ccgccgatgc
     1861 gatggaaaag cgcttgctgc cgcacgccga cacgatgccc atgctcgaat cgtttctctg
     1921 gacgcccatc tgggtggatc tgcgcttcgc gaaatggcag gaaatcctcg cgcgtccgga
     1981 accgcccgca aatcgaaaag ttcctcatct gatgtggcga tattcgcgaa cgatcgccta
     2041 tgcggcggaa cggcaagcgg cgaaggccga ggccgaacac gctctctatg cgaaagaggc
     2101 cggtttgctt ccgcatgaga ttccccttgg gcagatgaat cccaatgaat ccatgcttgc
     2161 agtgatgaat gagatcgttg cggccaaact gcatgccgcc aaaggcaacg cagagtctgc
     2221 actaaagcac tggcgcacag cggtcgaagc ccaagacaag ctgaactaca cggagccgcc
     2281 ggactggtat tatcccgtgc gagaatcgct aggtgcttcg ctgctggccg gtggcaacgc
     2341 gaaagaggca gaacaggact tccgggagga cttgcgtcgc aatccccgca acccgcgctc
     2401 cttgtttggc ttgaaggaaa gtcttcgtgc tcagcatttg gagaatgatg cagcgtgggt
     2461 ggatcgcgag ttccaaagaa cctggaagca tgcggactcg cagctgaaga ttggtgacct
     2521 gtactaactt aaaaaagcct tgctacaaag accaaagcgc cggatgggct ccttctcagt
     2581 ccacacggcg cttcagttgc gcttagtatc tgtttccact tgttccgttc gttcccctcc
     2641 tccgctcgcc gttcggccta gctaactagc gttttgaaca acccatcgtc ctcgcggctc
     2701 gaggaactcc acgccgatgg caaagccgtc gccttgcatc ggctggcagt aagccactct
     2761 tgccgaggaa cgaaactctc cggaacgcga agtgacggtg agccggtcgt tgggctccca
     2821 gcgccgcacg ctgattacgc gcgcgccacg ttcgctgaca ttttccgtga aggtagattc
     2881 ggcgcccggc acttgccggt tgccttccag atacacgccg atctccatgg ggatgcggcg
     2941 ttcgttacga tgaaagggga aagcttggcc gcgttgtgag ttcatggcca tcggctcctc
     3001 acaaacacgc cgcgatgcgc gagagatttt gaatgctgct gccccgatgc ttcatgcggt
     3061 agagcgcacg gtcggcagcc gccagcaatt tttccggcgt gtcgccgtcg tcggggaaag
     3121 ttgccgtgcc cgcgctggcg gagagaccag gcatctctac ctccgcggcc attttttcgt
     3181 gaatacggcc gacgatgcgg ctcgcaatgt ccgacccagc ctcgggaaac acaatggcga
     3241 attcgtcgcc gccatagcgc gctgcggtgt cgatcgcccg gcagtggttc cgcagaatct
     3301 tgcctaaacg aaccagcgcc cggctgcccg tcagatgccc atagcggtca ttgatcgcct
     3361 tcaggccatc catatcgaaa agcaccacgc taaacgggcg ctgcgtgcgg cgcgaacgat
     3421 caagttcggc ttcgatcacg ctcaacagac gtcgatagtt ggcgagcccg gtcagcggat
     3481 cactgactgc cagcgtgcgc acctgatcaa agagtctggc ctggtccagc agggcgccca
     3541 acagcatcac cacgtagctc gtcgtcttgg cgagttcggc tagaaagaat gggccgtcca
     3601 gaaggtggcg ggaaaatgtc gccgaaacgt gacaaaagac gttcagcgaa gccacgataa
     3661 aaagcgagta atcataaacg gacgcagact tcgcgtcctt gctcagccgc cagcgaaacg
     3721 ccattgcggc gacaaagaaa agcgctgcgg gaagcaagtc ccaggaacgc gaaagcaagc
     3781 gcgccggatg cgaacttggc gcggacgggg ctgccagaaa cgcggcgctc gtcgcgtagg
     3841 cgcacagcgc caccaccagc aacgccgcgg caatttcctt acttggctga cgcgaggtgg
     3901 gcgtatacgt ctccacggcc aaggctgcca gaagcagcac cgccagcaga gtgcggctga
     3961 ccatccaccc gaggggaatg tgcaagaacg cactgctgcc tgcatgcaga tactgcccga
     4021 aatggaaata accaacagtc tcgatcaaac cggaaagaac aaaaccaaac gaaagaatca
     4081 gagcgatgcg gtcgtgcgtc ccgcggaatc gcaccacggc attggctgca tagcagaagc
     4141 tgagcaacgt tccgcccact tccagataga ggtaagtctg ataactgccg gtaaaattgg
     4201 cagagcggaa ataaacgatc agcggaaagg ttgccaagcc cacgcccaaa aagcccaagg
     4261 tccacgccgc ggaaggccag cggcggagaa actcggcgat tcgggaccaa agtcgcaaga
     4321 aaacccccag agaccggaca aaaacctgca aagccggaag aatgcacgcc tgtgacctat
     4381 ccgggcggct ccacggggtc gctcaaaaag ctgaaaacaa aaggcttact tgcgggcgag
     4441 atttcaactg tgtaccgttt ggtcgcaagg cgagacgcga atcctacctc gccgagctgt
     4501 ctagcgcagt ttaagactcg tgtttacagc cagttacgtc tggattgggc acttttcctg
     4561 gggattagcc gcccagtctc agtgtgagac tgtctcaaag gggcccgctg ggctcgatcg
     4621 caatcagatc ttgggcttgg ccgccggcag ctcgcgggca accaccgtat ccgagaagcc
     4681 gctggtcttg tgggagccat cgcaaaaagg cttgttcgcg gagtgtccac atcggcatag
     4741 gccaatagcc gttcgtccag ccagaccgaa tgcctgtccc tgcgggtcca caatctcgaa
     4801 gtcgccttca attctgattg agccattatt attgacagta actttggtgg ctggcatctg
     4861 ctctccggta atcggtcatt ccgattttat ttctctcagg ctcccgcgct gtacatcgta
     4921 cacgaacccg cgcacctcaa tctgcttcgg caagaatgga tcgcgccgca cgcgcgccac
     4981 ttgttcccga acattctgat ccaggtcgtg gaacgcgtgg aattgcaggt gcgaagcgtc
     5041 tttgccagtg gaagtggaaa ggcgccagcg gaactcttcg tcggtgatcg acatcagtcc
     5101 gcattcggtg tgctgtatga ttgcgatcga gcgggtgccc agcaagtgcg tggaaagtat
     5161 cagcgaacgc accgcgtcat cagtcgccag cccgccggcg ttgcgaatca tatgcgcgtc
     5221 ccccacgcgc aagccgagca actgctcgag gtccatgcgc gagtccatgc acgtaaggat
     5281 gaccagcttg cgcacgggcg gtgtctgaag gtggcccagg tcaaagcgct gcgcgtagct
     5341 cgcgttatgg gcgagcatct cgtcaaacac gctcattgct cttttggcct gaatcggaag
     5401 tccgctactc agtatttggg aacgctcggg tccacttcat cgctccatgc aagaattccg
     5461 cccttcaggt tatgtatttt acggaagcct gccttgcgga gaaaatccac ggcttccgcg
     5521 gaacgcttgc cactacggca atgcgccacg atttcgcgcg aagagtccag ctcgtgtaag
     5581 cgccgtggca attcgccgag cggcagcaag tgtcccttga tgttgcagat ctggtattcg
     5641 tgcggctcgc gtacatctaa gatgtacaga tcgtcgccgc ggtcgagccg cgctttcagt
     5701 tcgcggggcg tcagttccgg tacctgcaga tcgggcgtgg gagcttcttc tccgcgcact
     5761 ccgcagaatt cgtaataatc tatcagttcg tgaatcgtcc ggttcttgcc gcacatcg
//
LOCUS       DASTPM010000247         3072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_787347, whole genome shotgun sequence.
ACCESSION   DASTPM010000247 DASTPM010000000
VERSION     DASTPM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787347"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFR42_04395"
     CDS             <1..481
                     /locus_tag="VFR42_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368424.1"
                     /translation="ATVLWCYLRNNAFKKDKDYHVSADLTGQANHLGVTIQADIIKQK
                     LPDVNGGYQALNIGDSSYGKFDKRIYTDLASDHPIDLCRYQVANCFMGRSGLINSGGA
                     SGANDFEEAVATAVINKRAGGTGLISGRKAFQRPMKEGVQLLNAIQDVYLSPEVTVA"
     gene            738..1304
                     /locus_tag="VFR42_04400"
     CDS             738..1304
                     /locus_tag="VFR42_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0368425.1"
                     /translation="MKKLIAGAVLTLAVSLGASFLFAPVASQAAPASASASQAVLSQW
                     NEIGRKLIAMAEDFPEDKYDFKATPAVRTFAEQLLHATGSMYYFTDPAQGQKPRYAED
                     PKRDSLKTKAEIVAFAKKCVEDGAAVIKSKGDKGMSELVTDPESKQQVQVSDLAYGLI
                     EHSGEHYGQLVVYYRLAGLVPPESRPKK"
     gene            complement(1329..>3072)
                     /locus_tag="VFR42_04405"
     CDS             complement(1329..>3072)
                     /locus_tag="VFR42_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368426.1"
                     /translation="DQGGRPGFRRVLLAVADSYRNKRADLERAANSLADAVAQAETFN
                     GARAKFDLSVVDAQMQSITKLFDIRNGGFGQSPKFPHASAIELVLERYQETKEKHLLA
                     MAETTLEKMARGGVYDQLAGGFHRYSVDERWLVPHFEKMSYDNSELLKNYLHGWQVTQ
                     NPFLRETAEGIIGWVNELLSDQRGGGFYASQDADYCLSDDGDYFTWTLEEVREVVLPA
                     EAQIIELYYDVEARGEMHHNPAKNVLWVAREPAAIAKTLGGDETAVRLTIARARDKML
                     AGRLRRITPYIDTTMYVSWNAMFVSAYLEAARILGRSIGDNCRDFALKTIDRMLNEAW
                     TEDKGFAHRIGGPHLEGSLDDQVFSVVALLDAYETTLHPRYFHAAQQTMDYLLENYAD
                     TEGGGFFDRSRKAAPLAGLDVRRKPFQDSPTPGANSVAIMALVRMHVFTGDERYYNFA
                     QKTLEAFAGIAPQYGLFAGTYGLAALMFSGHPVQVVITGRADDPAARSLEVAANRVYR
                     LGKSVLRFTGATGDDTAMENLPEALKETLPHLQGDKAVAIVCVGQSCLPPTADPTQLI
                     ALLENGASGAVGRS"
BASE COUNT          627 a          994 c          861 g          590 t
ORIGIN      
        1 cgcaaccgtg ctgtggtgct acctgcgcaa caacgcgttc aagaaggaca aggactacca
       61 cgtctcggcg gacctgaccg gccaggcgaa tcatctcggc gtcaccatcc aggccgatat
      121 catcaagcaa aaactgccgg acgttaacgg cggctaccag gcgctcaaca tcggcgattc
      181 cagctacggc aagttcgaca agcgcatcta cactgatctc gccagcgacc accccattga
      241 tctctgccgc taccaggtgg ccaactgctt catgggccgc tccggcctca tcaacagcgg
      301 cggcgcctcg ggcgcgaacg atttcgagga agctgtggct accgcggtca ttaacaagcg
      361 tgccggcggc accggcctca tctccggccg caaagccttc cagcgtccaa tgaaggaagg
      421 cgtccagctc ttgaacgcaa tccaggacgt ttacctcagc cccgaagtca ccgtcgccta
      481 actgcatcgc ggcgcaggtt ttccagggga aggagcgctt gtcatgcccg gttcggcaaa
      541 cggcgttcct gctttcccag ttaccctgga gaaactccgg cccgtttgca gattggctct
      601 attcctgagt actatcgaac gcgctgaagc cggaaacact ttcggccgaa cgccgttttc
      661 tcagtcagtc cgtcttgtcg atttgcagca gtgaaggcaa agcggcgtgg tccgctgtgg
      721 caaatcaagg agaaaaaatg aagaagttga ttgcaggcgc agtgctgact ctagctgtgt
      781 cgctcggggc ctcgtttcta ttcgcgccag tcgccagcca ggcggcgccc gcaagtgcaa
      841 gcgcgtccca ggcggtgctc tcgcaatgga atgagatcgg ccgcaaactg atcgctatgg
      901 cggaagattt tcccgaagac aagtacgact tcaaggccac gccggcggtg cgcacctttg
      961 ccgagcaact gctgcacgct acggggtcca tgtattactt caccgatccg gcgcaaggcc
     1021 aaaaacctcg ttatgcggaa gaccccaagc gcgacagttt gaagaccaag gcggagatcg
     1081 ttgcctttgc aaagaaatgc gtggaggacg gcgccgcggt tatcaagtct aaaggcgaca
     1141 agggcatgag cgagttggtc accgacccgg aaagcaaaca gcaggtgcag gtttccgatc
     1201 tcgcctacgg cctgatcgaa cactcgggtg agcactacgg ccagctggtg gtttactacc
     1261 ggctcgcggg acttgttcca ccggaatctc gcccgaagaa gtaggaaaca ccgaattcaa
     1321 gcagcctgct aggaccggcc gaccgctccg ctcgcaccgt tttccaggag cgcgattagc
     1381 tgcgtgggat cggccgtcgg cggcaggcac gattgcccta cgcatacgat ggcgacagct
     1441 ttatccccct gcaagtgcgg caacgtttcc ttcagcgcct cgggcagatt ctccattgcg
     1501 gtatcgtccc cggttgcacc tgtgaagcgc aacaccgatt tgccgagtcg atagacacga
     1561 ttagcggcaa cctccagcga ccgtgccgcg ggatcatccg cgcggcccgt gatcaccact
     1621 tgcaccggat gccctgagaa catcagcgcc gccagaccat acgtgcccgc gaacaacccg
     1681 tactgcgggg caatgcccgc gaaggcttcc agagtctttt gcgcgaaatt gtaatagcgc
     1741 tcgtcgccgg tgaaaacgtg catgcgcacc agcgccatga tggccacgga gttcgctccc
     1801 ggcgtcggcg aatcctgaaa tggcttgcgc ctcacatcca accccgccag cggcgcggcc
     1861 ttgcgcgacc gatcgaagaa tccgccgccc tccgtatccg cgtaattctc cagcaggtaa
     1921 tccatcgttt gttgcgcggc gtgaaaatag cgcggatgga gcgtggtctc gtaggcatcg
     1981 agcaacgcca caacgctgaa cacctgatcg tccagcgacc cctcgagatg cgggccgcca
     2041 atccggtgcg cgaacccttt atcttccgtc cacgcttcgt tcaacatccg gtcgattgtc
     2101 ttcagcgcaa aatcgcggca gttgtcgccg atggagcggc ccagaatgcg cgccgcctcc
     2161 aggtacgcgg aaacaaacat cgcgttccac gacacataca tggtcgtatc aatgtacggc
     2221 gtaatccttc gcaggcgccc cgccagcatc ttgtctctgg cgcgcgcgat ggtcaggcgc
     2281 accgcggttt catcgccgcc cagggtcttc gcaattgcag ccggctcccg cgccacccac
     2341 aacacgttct tcgccggatt gtggtgcatc tctccgcgcg cttcgacgtc atagtaaagc
     2401 tcgatgatct gggcctccgc cgggagcacc acctcgcgca cttcttccag cgtccaggtg
     2461 aaatagtcgc catcatcgga aaggcaatag tccgcatcct ggctggcgta gaatccgccg
     2521 ccgcgctggt ccgacagcaa ttcattcacc cagccgataa ttccttcggc ggtttcgcgc
     2581 aaaaacggat tttgcgtgac ctgccacccg tgcagataat ttttgagcag ctcggaattg
     2641 tcgtaactca tcttctcgaa gtgcggcacc aaccaacgct cgtctaccga gtagcggtga
     2701 aatccgccgg ctaattggtc gtacacgccg ccccgcgcca ttttttccag cgtggtttcg
     2761 gccatggcca gcagatgctt ttccttggtt tcctgatagc gctccagcac cagctcgatg
     2821 gccgaagcat gcggaaattt cggcgattgc ccgaagccgc cgttgcgaat gtcgaaaagt
     2881 ttggtgatcg actgcatctg cgcgtcgacc acgctcagat cgaacttggc ccgcgcgccg
     2941 ttaaaggtct ccgcttgggc cacggcgtcg gccagcgaat tggccgcccg ctccaggtcc
     3001 gcccgcttgt tgcgatagga atcggcgacc gccagcaaca cccggcggaa gcccggccgc
     3061 ccgccctgat cc
//
LOCUS       DASTPM010000248         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_556699, whole genome shotgun sequence.
ACCESSION   DASTPM010000248 DASTPM010000000
VERSION     DASTPM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556699"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /gene="lpxK"
                     /locus_tag="VFR42_04410"
     CDS             complement(<1..733)
                     /gene="lpxK"
                     /locus_tag="VFR42_04410"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU0368427.1"
                     /translation="MDWPLPIRILLLPLSWVYGLVVRCKTILYGSRILQPKRLKGAVI
                     SVGNLTVGGTGKTPMVLWLAEKFLGEGKRVAILSRGYQGSAGTSDEIELLKSRLEGRV
                     RFGVGADRFAEGSKLEQAEPVDVFLLDDGFQHLKLARDLDIVMLDGSKRLTNEWLLPA
                     GSLREPINACRRSDLLVVTRKFERPPIEANDSHEHQLFYAQTRLLGYRHMGEPATPLY
                     LSEIGPGPFFAFCGIGNPQAFFADLK"
     gene            complement(734..2101)
                     /locus_tag="VFR42_04415"
     CDS             complement(734..2101)
                     /locus_tag="VFR42_04415"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEU0368428.1"
                     /translation="MYLVYSLLMGLAAILLTPYWLVQGIRHKKYLTNLNERLGLSLPA
                     LGKLPAERKGALWLHAVSVGEVLAGVTLAKRLKEEFPERPLVVSTTTITGQSLAKERM
                     AFADAVIYFPLDWQFCVRRALAAVKPALIVVLETEIWPNFLREASDHNIPVLFVSGRI
                     SDRSFARSQSWLSLFGFYLRPFWKDALGRAAGFWMQSERDAERVKALGAPAANVRVGG
                     NLKYDLELPKNTPLADWLEGEVARDGRRPVIVAGSVVATEEPLALIAFGVLQGEHRNA
                     LLVLAPRKPERFEAAAQFIEDSHQKWIKRSGLRVASPSGPNAKGGETAIASDVAVLLL
                     DSIGELASLYRLADGVFVGGSLVESGGHNILEPAAFGKVPVFGESMENFAEMAERFVT
                     AEAALQVASPEDAGVSWIELLRNPERNKKMGETARRLVEESRGATERAVTAIRGELDK
                     VHAAD"
     gene            2174..2863
                     /locus_tag="VFR42_04420"
     CDS             2174..2863
                     /locus_tag="VFR42_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368429.1"
                     /translation="MQKVYYTRMKIASSAVISVALPLTMFILSAKPPASAQQQSASKP
                     ITTASLDSHEGLTIAADPWLSAERYQQAFPKKSPYAAGILAIKVTLRNDSAESIRVDI
                     ERIRLNLTFDDNNRQELPGLTSEQLADAVLHPRVKSPSKPRIPLPLPSSSGGRDKNWE
                     QFQKLAEDNGLHASILAPHSTTEGLLYFDLQNQFDLLANARLYIPNLFALEKNQALMY
                     FDIDLSRPGSR"
     gene            2989..>3381
                     /locus_tag="VFR42_04425"
     CDS             2989..>3381
                     /locus_tag="VFR42_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0368430.1"
                     /translation="MQHLLNSLNPEQRLAAETTEGPVLLLAGAGTGKTRAITFRMANL
                     ISRGVPAESILAVTFTNKAAEEMQSRVSDLLLKAGVPPERPWLSTFHSLCARLLRREA
                     ASAGLPSNFSVFDDSDQLAAIKLAMTALN"
BASE COUNT          708 a         1176 c          795 g          702 t
ORIGIN      
        1 gtttgagatc tgcaaaaaac gcctgcggat tgccaatccc gcaaaaagca aaaaacggtc
       61 ccggtccgat ttcactcaaa tagagcgggg tcgctggctc gcccatgtgc cgatatccta
      121 gtaagcgggt ctgtgcataa aacaactggt gctcgtgcga atcgttggcc tcgatcggcg
      181 gacgctcaaa cttgcgtgtc acgacaagca aatccgacct gcgacacgcg ttgatgggct
      241 cgcgcaagct tcccgccggc aaaagccatt cattcgtcaa tcgtttcgag ccatccagca
      301 ttacgatatc caggtcgcgt gccaacttca gatgctggaa gccgtcgtcc agcagaaaaa
      361 cgtcgacagg ctccgcctgc tcgagtttgc tgccttcggc aaaccggtcc gcgccgacac
      421 cgaatcgcac acgcccctcc aaccggctct tcaacaactc aatctcatcg ctcgttcccg
      481 cgctgccctg atatccgcga ctcagaattg cgacgcgttt cccttcgccc agaaattttt
      541 ccgccaacca cagcaccatc ggtgtcttac ccgtgccgcc gacggtcaga ttaccaacac
      601 tgatcactgc gcctttaagc cgtttcggct gcaaaatcct gctgccatag agaattgttt
      661 tgcagcgcac gaccagtcca tacacccacg acagaggtaa aagcaaaatc cggattggca
      721 gcggccagtc catctaatct gctgcgtgca ccttgtcgag ttccccgcga atcgcggtaa
      781 ctgcgcgctc agtcgcgcct cggctctctt ccaccagccg ccgcgccgtt tctcccatct
      841 ttttgttccg ctccgggttg cgcagcaact caatccacga cacgcctgca tcctcgggac
      901 tcgccacttg cagcgccgct tccgcggtca cgaatcgctc cgccatctcc gcaaaatttt
      961 ccatggattc gccgaacacc ggcactttgc caaaagccgc tggctccaaa atgttatgcc
     1021 cgcccgattc caccagcgag ccgcctacaa atactccgtc cgccagccgg tagagcgacg
     1081 ccagttcgcc aatactgtcc agcaaaagca ctgctacgtc gctggcaatt gccgtttcgc
     1141 cgcctttggc gttcggcccg gagggcgatg ccactcgcaa acccgatcgc ttgatccatt
     1201 tttggtgcga atcttcgata aactgtgccg ctgcctcaaa acgttccggc ttgcgcggcg
     1261 ccagcaccag cagcgcgttg cggtgttctc cctgcagtac gccgaacgcg atcagcgcca
     1321 gcggttcttc cgtagccacc acgctgcccg caacaatcac cggcctccgc ccgtcgcgcg
     1381 ccacctcgcc ttccagccag tcggccagcg gcgtgttttt tggcagctcc aggtcgtatt
     1441 tcagatttcc acccacgcga acgttggccg ccggcgcacc cagcgccttc actcgttccg
     1501 cgtcccgttc gctttgcatc cagaaaccgg cggcgcgtcc cagcgcgtct ttccagaagg
     1561 gtcgcaagta aaatccgaat agactcagcc acgattgcga gcgcgcaaac gaacggtctg
     1621 aaatccgccc gctgacgaac aggacgggaa tgttgtgatc cgaagcctca cgcaagaaat
     1681 tcggccagat ttccgtttcc agcacgacga tcaatgcagg cttcacagcg gctagtgctc
     1741 tccgaacaca aaactgccag tccagcggga aataaatcac cgcatccgcg aacgccatcc
     1801 gctctttcgc cagcgattgt cccgtaattg tcgtagtcga aaccaccagc ggccgctcgg
     1861 gaaactcttc cttgagccgt ttggccagcg tcacgcctgc cagcacttcg cccaccgaca
     1921 ccgcatgcag ccacagcgcg ccctttcgct cggcgggtaa tttccccagc gctgggagcg
     1981 acaagcccag cctttcattc aaattggtga ggtatttttt gtgacgaatg ccttgcacca
     2041 gccagtatgg cgttagcaga atcgcagcaa gccccatcag caggctgtaa acgagataca
     2101 ctccgcaagg ctccggtctg acccgtgggc gcagggcaac cctgcacaca acccaggaat
     2161 cgaaggcgtc gagatgcaaa aagtctatta tacccggatg aaaatcgcct cctcggccgt
     2221 tatctccgtt gctctgccgc tgacgatgtt catcctgtca gccaagccgc cagcaagcgc
     2281 gcaacagcaa tccgcttcca agcccatcac caccgcctct ctcgattcgc acgaaggtct
     2341 gaccattgcc gctgacccgt ggctctccgc cgagcgctac cagcaggcgt ttcccaaaaa
     2401 gtcgccgtac gccgcgggaa tcctcgccat caaggtcacg ctgcgcaacg actccgccga
     2461 aagcatccgc gtcgacatcg aacgcattcg cctgaatctc acgtttgacg acaacaatcg
     2521 tcaggaattg ccggggctca cttctgaaca acttgccgac gccgtactgc atccccgcgt
     2581 gaaaagtcct tccaagccgc gtattccgct tcccttgcca agttcttccg gaggtcgcga
     2641 caaaaattgg gagcaattcc agaagctcgc cgaagacaac ggtctgcacg ccagcatcct
     2701 cgcgccgcat tccactaccg aaggtcttct ctatttcgat ctacaaaacc aattcgatct
     2761 gctcgctaac gctcgtcttt acattcccaa tctgtttgcg ctcgaaaaaa atcaggcgct
     2821 gatgtatttc gacatcgatc tcagccggcc gggttcacgc tagctctcca cactccgctc
     2881 gcgggagttt cgagatttcg tttgccattt tccttagcaa attttcacaa atttgcgccg
     2941 tctctctccg tccgttaaca tcgttgagtc actttcttcg accgacccat gcaacacctt
     3001 ctcaactcgc tcaatccgga acagcgtctc gccgcagaaa ccaccgaagg tccggtgcta
     3061 ttgctcgccg gcgccggtac cggcaaaact cgcgccatca ccttccgcat ggccaatctc
     3121 atttcgcgcg gcgtccccgc cgaatccatc ctcgcggtca ccttcaccaa caaagcagcc
     3181 gaggaaatgc agtcgcgcgt ctccgacctt ttgctgaaag ccggtgttcc acccgaacgc
     3241 ccgtggctct ccacgtttca ttccttgtgc gcgcgtttgc tgcgccgcga agccgcctcc
     3301 gccggcctcc cgtcaaactt ttccgtcttc gacgattccg accagctcgc cgcgatcaaa
     3361 ctcgcaatga ccgctctcaa c
//
LOCUS       DASTPM010000249         6765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_152743, whole genome shotgun sequence.
ACCESSION   DASTPM010000249 DASTPM010000000
VERSION     DASTPM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152743"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2898)
                     /locus_tag="VFR42_04430"
     CDS             complement(<1..2898)
                     /locus_tag="VFR42_04430"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368431.1"
                     /translation="MKRLLQRAKTKKCAGKPGLLIALFLCASTGLLVTSLRGFSATPQ
                     ISMGQSSQEADRKSAGCITCHWPMDEPTMHPTKTVQLGCTDCHGGNSSAGITPGTAQN
                     SSEYLARKEKAHVQPRDPGFDRGALPKRVFTKWLGESAEYVKFVNPGDLRVAAETCGS
                     AGCHASETRAVSTSMMTHTGMLWNAALYNNGGIPTKNTRFGESYDRDGKPQSLKTVPP
                     PSDQEIRAKGVLPELSPLNRWETSQPGNVLRVFERGGEKKAELGNPTRAEQPGKPDDK
                     LSDRGFGTELRTDPVFLGLQKTRLVDPVMSLPGTNDHPGDYRASGCSACHVIYANDRD
                     PAHSASYSAFGHSGAGATVDPTIDKSQPGHPIKHTFTRSIPSSQCMVCHMHPGTNMLT
                     TYFGLTWWDNEIDGERMYPAKQKNPTEEERYQSGLRNPEGAAARGLWGEEKFLDQTGS
                     PEFNRQLKTTQFADFHGHGWVYRGVYAHDRKGNWLDKSGAKIAFDDPDRFGKAVHLAD
                     IHLEKGMQCNDCHFAQDNHGNGKIYGEPRAAVEIDCTDCHGTINAPALLITSGPAAPP
                     GGRQLEALRTPWRQRRFEWRGDKLIQRSMSDEHKEWDVVQTRDTVTPGNVHFSPKSYR
                     AKLMSKSGWPFAAVPPEAAQLAHANNAMTCYTCHTSWTPTCFGCHLQMTANAKRPMLH
                     NEGTTTRNYTSYNFQVLRDDIYMLGIDGTVTGHRIAPARSSCAVLVSSQNANREWLYY
                     QQQTISAPGLSGQAFSTFVPHTVRAKETKQCSDCHVSAANDNNAWMAQLLLQGTNFMN
                     FMGRYIYVATGHHGLEAVAVAEHDEPNAIYGSDLHRIAYPDDYKKFVARGRELRTTYE
                     HPGTVLDVQARGEYAYAAQGKGGFRVFDIANIDNKGFSERMTTAPVSPLGQKFFVPTK
                     DAVAVASPTTLGVDPLRVQLPENEEQPVPPLYGFLYVADSEEGLVVIGNPNL"
     gene            complement(2903..5113)
                     /locus_tag="VFR42_04435"
     CDS             complement(2903..5113)
                     /locus_tag="VFR42_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0368432.1"
                     /translation="MRLPAGLYWRGLIGLILVSAAFSAHAFPKQQAQQSQEAGPAVRI
                     PRQQAQITAALDGVVRSGSAASSPQLPVSGALLTLRNAATNVTTQVAANGEGVFRVFP
                     LPPGDFALQVHAEGFAEFALERLTLHADEVLTLEISLPPAATTELRSRLPRWPELGPP
                     PTQAAEVLSPSYREFRHRMDWDANYVLNLAPESLPPAADVFATVPDRWALHQPEYRRY
                     SAPGEYVYSKPRWYDPFNRNRFKGDEPIWPSLLGQQVFLNITAASDTTFDARRVPSPS
                     NVSAANPGSDEFFGRGEQEFVDQTLRFSFDLFHGDTSFKPVDWRARITPEISINDLNV
                     RELGIVGPDVRSGTNRFDTHVGLQEAFAEVKLHDLGPNYDFVSLRAGIQQFNADFRGF
                     LFVDEQPAVRIFGDLFSDKIEYNAAYFHFLEKNTNSGLNTFERRHQQVVLGNVYLQDF
                     FFPGYTAEFVAAWNKDDATIHYDDNGFLVRPAPIGNVINQNPGAGPIPHAIRVGYFGW
                     LGSGHIHRFNITHAFYQAVGEDTFNPIAGRPVTVNAQLAAAEISYDRDWVRYRVSAFY
                     TSGDANPRDSRARGFDSILDLDSFAGGLFSFWNREGFRLTGSGVLLTTPGSLIPSMRA
                     SKDEGQANFVNPGIFLLNAGADFDITPKLKGFANVNFLRFERTEPLEFALFQGPIRHA
                     IGVDYGAGVTYRPPLSENIVLTGGTAMLTPGDGFRDIYGGKTLFSLFGSVKFAF"
     gene            5453..>6765
                     /locus_tag="VFR42_04440"
     CDS             5453..>6765
                     /locus_tag="VFR42_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368433.1"
                     /translation="MFALAAAGAMALLFAGCGGGSGTVATPTPPSPSVQPLQIADVQK
                     IVQAAVNSANADMVVAVVDRAGFVLGVFRTQNAPSIAIGNFSATVDANDLAVALARTG
                     AFFSNNRAPLSSRTVRFISGIHFPAGVQNQPPADLYGIENTNRGCSLVNDPTFESRIA
                     PSLALGGGFGPGVITGKKDVRDSDPNVVNPGGLPIFYQNVVLGGVGVVTSSTNLNVAE
                     YAALVGATTQRGAAGDNFGPTPAAPGVVFIGGIALPFVLQITLPAGFSAGPATGTGAF
                     LVAPSNSAGQPPEGDLIPPAAGPLGGLSATDVKQILDNAETEANLTRAAIRLPLGSRT
                     RMVISVADLDGTIIGLRRMPDSTVFSIDVAATKARNMVYFNGPNRLSSDLNQVPMGTA
                     VTNRTISFGAEPLFPAGIDGSGSGPFFNLYTMDTANPCTQGFQTGA"
BASE COUNT         1360 a         1890 c         2090 g         1425 t
ORIGIN      
        1 gagattcgga ttgccgatga caacgaggcc ttcttcgctg tcggcgacgt agagaaaacc
       61 ataaagcggc ggaaccggct gttcttcgtt ttcgggaagt tgcacgcgaa gcggatccac
      121 gccgagcgtc gtgggtgagg caacggccac cgcgtctttg gtgggaacaa agaatttctg
      181 gcccagcggc gagacggggg ccgtcgtcat acgctctgag aagcctttgt tgtcaatgtt
      241 ggcgatgtca aagacgcgga agccgccttt tccttgcgcc gcgtaggcgt attcgccgcg
      301 tgcctggacg tcaagtaccg tacccgggtg ttcgtacgtg gtgcgcagtt ccctgccgcg
      361 tgcgacgaat ttcttgtagt cgtcgggata ggcgatacgg tgcaggtcgc taccataaat
      421 cgcgttgggc tcgtcgtgtt cggcgacggc aacggcttcg aggccgtggt gtcccgtcgc
      481 gacgtaaatg tagcggccca tgaagttcat gaaattggtg ccttgcagaa gcagctgcgc
      541 catccaggcg ttgttgtcgt tggcagcgga aacgtggcag tcgctgcact gttttgtttc
      601 tttcgcgcga acagtatgcg gcacgaaagt gctgaaggcc tgaccggaaa ggcccggcgc
      661 ggaaatggtc tgttgctgat agtaaagcca ttcgcgattg gcgttttgcg agctgaccag
      721 cacagcgcag gaggagcgcg cgggcgcgat acgatggccg gtgacggtgc cgtcgatacc
      781 gagcatgtaa atgtcgtcgc gcagcacctg aaagttatag ctggtgtagt tgcgcgtggt
      841 ggttccttcg ttgtgcagca tggggcgttt ggcgttggcc gtcatctgca agtggcaacc
      901 gaagcaggtc ggcgtccacg aagtgtggca ggtgtagcag gtcattgcgt tattggcgtg
      961 agctagctgg gcagcttcgg ggggaactgc ggcgaacggc caaccggact tgctcattaa
     1021 cttggcgcga taggatttgg ggctgaaatg cacgttgccg ggagtgacgg tgtcgcgtgt
     1081 ctggacgacg tcccactcct tgtgttcgtc gctcatggag cgctgaatca gcttgtcgcc
     1141 gcgccattcg aagcggcgtt gcctccacgg cgtgcgcagc gcttcgagtt gccggcctcc
     1201 tggaggagct gcgggcccgg aagtgatgag cagagctggc gcgttgatgg tgccgtggca
     1261 atcggtgcag tcgatttcga cggcggcacg cggttcgcca taaatctttc cgttgccgtg
     1321 attgtcctgc gcgaaatggc aatcgttgca ctgcataccc ttttccaagt gaatgtcggc
     1381 caagtggacg gcctttccga aacggtcagg atcgtcgaaa gcaattttcg cgccgctctt
     1441 gtcgagccaa ttgcccttgc gatcatgcgc gtagacgccg cgatagaccc agccatggcc
     1501 gtggaaatcg gcgaactgcg tggtcttgag ctgcctgttg aattccgggc tgccggtttg
     1561 atcgagaaat ttttcttcgc cccacaggcc gcgagcggcg gcgccctccg gattgcgcaa
     1621 tcccgattga tagcgctctt cctcggtagg atttttctgt ttcgcgggat acattcgctc
     1681 gccatcaatt tcgttgtccc accaggtcaa gccgaagtag gtggtaagca tgttcgtgcc
     1741 ggggtgcatg tggcagacca tgcattggct ggagggaata gaacgcgtga aggtgtgttt
     1801 gatcggatgg ccgggctgcg acttgtcaat cgtcggatca actgtggcgc cggcaccgga
     1861 atgcccgaag gccgaatacg acgcggaatg ggcggggtcg cgatcgttcg cgtaaatcac
     1921 atggcacgcc gaacagccgc tggcgcgata gtcgccggga tgatcgttgg ttccgggcaa
     1981 ggacatcacg ggatcgacga gccgcgtttt ctgcaggcca agaaaaacgg gatcggtgcg
     2041 cagttcggtg ccgaagccgc ggtcgctgag tttgtcgtca ggcttgccgg gctgctccgc
     2101 ccgtgtagga tttccgagtt cagccttctt ttcgccgccg cgttcgaata cacgcaacac
     2161 gttgccaggt tgcgaagttt cccagcgatt gagcggcgac aattcaggaa gcacgccctt
     2221 cgcgcgaatc tcctgatcgg atggcggtgg aacggttttg agcgattgcg gcttgccgtc
     2281 gcgatcgtaa ctttcgccga atcgggtatt ttttgtcggg atgccgccgt tgttgtagag
     2341 cgccgcgttc cagagcatgc cggtgtgcgt catcatgctg gtggaaacgg cgcgtgtttc
     2401 actggcatgg cagcccgcgc tgccgcaggt ttcggccgcg acacgcaagt cgcccggatt
     2461 tacaaatttc acgtactcgg ccgattcccc aagccattta gtgaaaactc tcttcggcag
     2521 agcgccacga tcgaaaccgg gatcgcgcgg ttgtacgtgg gccttttctt tcctagcgag
     2581 atattcagat gaattctgtg cggtgcctgg cgtaatccct gctgaggaat ttccgccgtg
     2641 gcagtcggtg cagccgagct gcacggtttt tgtgggatgc atggtgggct cgtccatcgg
     2701 ccaatggcag gtgatgcagc cagccgattt gcggtccgct tcttggctag attggcccat
     2761 gctgatttgc ggcgttgcgg aaaatccgcg caaactggtg acgagcagcc cggtcgacgc
     2821 gcacaagaat aaagcgatca acaagccggg tttgccggca catttcttcg tcttcgctcg
     2881 ttggagtagt cgcttcatca ggtcagaagg caaacttcac cgagccgaat agcgaaaaca
     2941 gagtcttgcc gccgtagatg tcgcgaaagc cgtcgcccgg cgtcagcatc gcggttccgc
     3001 cggtgaggac aatattctcc gaaagtggcg ggcggtaagt cacacccgcg ccgtaatcca
     3061 cgccaatggc atggcgaatc ggcccctgaa agagggcaaa ctcaagcggc tcggtgcgtt
     3121 cgaagcgaag aaagttcacg ttcgcaaaac ctttcagctt cggggtgatg tcgaaatccg
     3181 cgccggcatt gaggagaaaa attcccggat tcacgaaatt ggcttggcct tcgtctttgc
     3241 tggcgcgcat ggacggaatg aggctgcccg gtgtggtcag caggacgccc gaaccggtca
     3301 gacggaaacc ttcgcgattc cagaaactga agaggccgcc ggcgaatgaa tccagatcga
     3361 gaatggaatc gaagccgcgt gcacggctat cgcggggatt agcgtcaccg ctggtgtaaa
     3421 acgcggagac acgatagcgc acccagtcgc ggtcgtagga aatttcagcg gcggcgagtt
     3481 gcgcgttgac cgtgaccggc cgacccgcga tgggattaaa cgtgtcttcg ccgacggctt
     3541 gataaaacgc atgcgtaata ttgaagcgat ggatgtgacc gctgcccagc cagccaaagt
     3601 atccgacgcg aatcgcgtga ggtatgggcc ccgctccggg attctggtta atgacatttc
     3661 caatcggcgc aggccgcacg agaaacccgt tgtcgtcata gtgaatcgtc gcgtcatcct
     3721 tgttccaggc agcgacaaat tccgctgtgt aacccggaaa gaagaaatcc tgcaggtaga
     3781 cattgcccaa cacgacctgt tgatggcggc gttcgaatgt attcagcccg ctgttggtgt
     3841 tcttctcgag aaaatggaag tacgcggcgt tgtattcaat tttgtccgaa aacaaatcgc
     3901 cgaagatgcg gacagcgggt tgttcatcca cgaatagaaa accacgaaag tcggcgttga
     3961 attgctggat acccgcgcga aggctgacga aatcgtagtt cgggccgaga tcgtgcaact
     4021 tcacttccgc gaaagcttcc tgcagtccga cgtgcgtatc gaagcgattg gtgccgctgc
     4081 gcacatcggg tccgacgatg cccagttcgc ggacgttaag atcgttgatg ctgatttccg
     4141 gcgtaatgcg ggcgcgccag tccaccggtt tgaacgaagt atcaccgtga aacagatcga
     4201 agctgaagcg aagcgtctga tccacgaatt cctgttcacc acgtccgaag aattcatcgc
     4261 tgccgggatt cgccgcgctg acattgctgg gtgaaggaac tcggcgcgcg tcgaaggttg
     4321 tgtcggaggc ggccgttatg ttcaggaaaa cttgctggcc aagcagcgag ggccagatcg
     4381 gctcatcacc cttgaagcgg ttgcgattga atggatcgta ccagcgcggc ttgctgtaga
     4441 catactcacc cggagcagaa tagcggcgat actctggctg gtgcagtgcc cagcgatccg
     4501 gcaccgtcgc gaaaacgtcg gcggccggcg gcaaactttc cggtgccaga ttcaaaacgt
     4561 agttggcatc ccaatccatg cgatggcgaa attcgcgata actgggggag agcacttcgg
     4621 ctgcttgagt cggaggaggc cccagctcgg gccagcgtgg aaggcgtgag cgaagttcgg
     4681 tggttgctgc gggcggcagc gaaatctcca acgtgagaac ctcgtctgca tgcagcgtca
     4741 gcctttccag ggcaaactcg gcaaagcctt ccgcgtgtac ctgcaatgca aaatcgcccg
     4801 gcggcagcgg gaagacgcga aagacacctt ctccattcgc ggcgacttgc gtggtcacgt
     4861 tcgtagcggc gtttcgcaat gtgagcaacg cgccactcac cggcaactgc ggcgaggacg
     4921 cggcgctccc cgatcgcaca acgccgtcga gtgccgctgt gatctgcgct tgctgacgcg
     4981 ggattcgcac ggccgggccc gcttcctgcg actgctgcgc ttgttgcttt ggaaaagcat
     5041 gcgcagagaa tgccgcgctg accaaaatca agccgatcag cccgcgccag tacagaccag
     5101 ctggaagccg cacgggacgc gaagataatg tctgcatgca ggttgcctga aagtgtcgct
     5161 ctgaaaacga cgcccgaaag cgccgcaaga aaaagaagtc tcgtatagct aacaacacac
     5221 accgcaggcg agcaacgttt ttcaaaacgg atgaggacag tcaataaccg gagttgccgg
     5281 cgccctaagg ttcactgttt agcgccaatc tgcgcggcga agattgagcg cgtgtacctg
     5341 ctgtgttacc aacactagta tcgttccgat gaaaaaagtc agagagctcc ggcgtccctt
     5401 gcgatcgcag cgcttgttgg agagacgccg ctgcattctc gcgtttggcg ccatgttcgc
     5461 tctcgcagcc gctggcgcga tggcattgct tttcgcaggc tgcggagggg gcagcggcac
     5521 tgttgccact cccacgccgc cctcgccctc cgttcagcct ttgcaaatcg ccgacgtgca
     5581 aaaaatcgtg caagccgcgg tgaactcggc gaacgcggac atggtagtcg ccgtcgttga
     5641 ccgcgcaggc tttgtcctcg gcgtttttcg cactcaaaat gcaccgtcga ttgccattgg
     5701 aaatttcagc gcaacggttg atgccaatga cctggcggta gcccttgcgc gcaccggagc
     5761 atttttcagc aacaatcgag ctccgctcag ttcgcgtacc gtgcggttca tcagcggcat
     5821 tcatttccct gccggcgtgc aaaaccagcc acccgccgat ttatatggca tagaaaatac
     5881 caaccgcggc tgctcgctgg tcaacgatcc gacattcgag agccgaattg ctccctcgtt
     5941 agcgctgggc ggcggattcg ggccgggcgt catcaccggt aagaaggatg tgagggattc
     6001 tgatccaaac gtcgtaaatc ccggcggctt gccgattttt tatcagaacg tcgtactggg
     6061 cggggtgggt gtcgtaactt cttccaccaa cctgaacgtg gctgagtatg cagcgctggt
     6121 gggagcaact acacagcgcg gagcggcggg cgacaatttt ggaccgacgc cggcggcgcc
     6181 cggagtcgtc ttcatcggtg gcatcgcgtt gcctttcgta ctgcaaatca cacttcccgc
     6241 cggattctca gcgggtccgg cgaccggcac tggggccttt ctggtcgctc cttcaaatag
     6301 cgcgggccaa cctccggaag gcgacttgat cccgccggcc gctggtccac tgggtggttt
     6361 gagcgcgacg gatgtcaaac aaatcctcga caatgcggaa acggaagcca acttgacgcg
     6421 agccgccatt cgccttccct tgggctcccg cacgcgcatg gtcatttccg tggccgacct
     6481 cgacggtacg atcatcggcc tgcgccgtat gccagactca accgttttca gcatcgacgt
     6541 tgccgcaacc aaagcccgca acatggtcta tttcaacgga cccaatcgtc tttcgtccga
     6601 tttgaatcaa gtgccgatgg gaaccgccgt aactaaccgc acgatcagct tcggcgcgga
     6661 acctttgttt ccagccggga tcgacggcag cggctccgga cctttcttca acctctacac
     6721 gatggacaca gcgaacccct gcacgcaggg atttcaaacc ggagc
//
LOCUS       DASTPM010000250         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_960169, whole genome shotgun sequence.
ACCESSION   DASTPM010000250 DASTPM010000000
VERSION     DASTPM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960169"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VFR42_04445"
     CDS             complement(<1..1119)
                     /locus_tag="VFR42_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0368434.1"
                     /translation="MQKSGSTQASPTTGLLLGLVLTLAAVLVYSFYTRSQLTGLRKLQ
                     SEMVDRNRKDSLQLLRVQNDLNSLGLAMRDMLDAREPYPLTAWSSQFQRIREDLDAAL
                     KAEARVSPATRTEDQRQFLNQAVAQFWDAVDRMFELAASGKEKEARDQIQLTLQARQA
                     ALSTAVSRLLVQNNEGEQQAAEQIAKIYNGVQRQLYLLLGATLIAIVLTSLYLIRSNR
                     RIFARIELLSEQRSELAQKLIHTQESTLHHISRELHDEFGQVLTAIGTLLGRAGKQVP
                     ENSPLHGELKEVREITQSTLNNIRSLSQALHPVLLEEAGLESTLDWYIPTVERQMGIS
                     LHYEKSGLAFTVDSAAGVQIYRIAQEALNNVSRHAGVHE"
     gene            1308..3296
                     /locus_tag="VFR42_04450"
                     /pseudo
     CDS             1308..3296
                     /locus_tag="VFR42_04450"
                     /inference="COORDINATES: protein motif:HMM:NF016563.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-lysosomal glucosylceramidase"
BASE COUNT          680 a          833 c         1049 g          734 t
ORIGIN      
        1 ctcgtgaacg ccggcgtggc ggctgacgtt gttgagcgct tcttgggcga tgcgataaat
       61 ctgaacgccg gccgccgaat ccaccgtgaa cgccagcccc gatttttcgt agtgcaatga
      121 aattcccatc tgccgctcca cggtgggaat ataccagtcg agcgtgcttt cgaggccggc
      181 ctcttcgagc agcaccggat ggagcgcttg cgacaggctg cgaatgttgt tgagcgtgga
      241 ctgggtgatt tcgcggactt ctttcaattc gccgtgcagc ggcgaatttt ccggcacttg
      301 tttgccagcg cggccgagca gtgtgccgat tgccgtcagc acctggccga attcgtcgtg
      361 cagttcgcgc gaaatgtggt gcagcgtgga ttcctgggtg tgaatgagtt tttgcgcgag
      421 ttcgctgcgc tgttcggaga gcagctcgat gcgggcgaag atgcggcgat tcgaacgaat
      481 cagatacaag ctggtcagaa cgatcgcgat tagtgttgct ccgagcagca gatacaattg
      541 ccgctgcacg ccattgtaga ttttcgcgat ttgttccgcc gcttgctgct cgccttcgtt
      601 gttctgcacc agcaggcgcg agaccgccgt gctcagcgcg gcttggcgag cttgcagggt
      661 cagctgaatc tgatcgcggg cttccttttc ttttccggag gcggccagtt cgaacatgcg
      721 gtccaccgca tcccagaatt gcgctacggc ttgattgagg aattggcgct gatcttcggt
      781 gcgggtggcg ggcgaaacgc gggcttccgc tttcagggcc gcgtccaggt cttcacgaat
      841 gcgctggaac tgcgagctcc aggcggtgag cggataaggc tcacgggcgt cgagcatgtc
      901 gcgcatcgcg aggcctagcg agttcaagtc gttctgtacg cgaagaagct ggagcgagtc
      961 cttgcgattg cggtctacca tttcgctttg cagtttacgc aagccggtga gttgggagcg
     1021 cgtataaaac gaatacacaa gaacggcggc cagcgtcagc accaggccca gcagtagtcc
     1081 ggttgtcggc gaagcttgcg tcgaaccgct tttttgcacg gagtagacga taacactgca
     1141 agtgcaactg ttgtagcggc ggcccttcgt gcttctaatg gaaacgcaat ccttgtgtag
     1201 aataacgatt ttgtatagca tcctcgcacc ggttgttggt cgctgttgcg caccctgcca
     1261 ccagcaatcc atcacgcgca gcgttttttt tttgggggag gctcgccatg gttcgccgat
     1321 ttgctctggt cactttgtgt gtgctttcct gtatagccac atcggcggcg tccgcggacg
     1381 atattcccaa ggcagcctgg aagcgaccga ttgggctggc gcttgagaat gcgggcacgc
     1441 gcaagcccgc gctcgacaac ggtcacgtcg acgacagtta ttggcagggc gcgccggtgg
     1501 gtggattcgg cgcggggact ttttcgcgga cgtttcgagg cgatttttcg cgctggcatt
     1561 tgcaggctgg cgtgcaccgc taccagaccg tttatgccaa ccagtttgca atgtttcaga
     1621 aatccgaaag cgacgaggcc ggtacggcga aagttctgtt tgctggacat ccgaacaacg
     1681 gatccctgaa aagctggggc tggggttatc cggtgggcgc gggcgattat gctgcgctgt
     1741 acccgaagtc gtggttcgac tatcgctggg ataagtttcc agcgcacgtg accgtggagc
     1801 agttctcgcc ggtgctgccg gacaactaca aagagtctag ctaccccgtg gcggtgtatc
     1861 gctggcaggc ggaaaatccg acgagtaaaa aggtgacggt gagcgtgctg ctatcgtgga
     1921 ccaacatgct gggctggctg cgcggttacg accgcgattt tcgtggcgcg ctgaatgaag
     1981 ggaacttcaa tcgcttccat tctgaaaaaa ttggcgacgg cgcgatgaag ggaatcgtgt
     2041 tcgaacggaa tcgcggcagc aaggaggcta acgcatggga cgggcaaatg accatagcgg
     2101 cgctggagtc gtccggtgtg gaaatttctt atgtcacgac gttttcgccc gacggtcccg
     2161 gcaacgaggt gtgggactcg ttcgctaaag atggccgctt gtctgattcg aatcagccgt
     2221 ggcttagcag cggcgaaccg ctggccgggg caatcgcggt gcgcttcacg ctggcgccgg
     2281 gcgaaaagaa aaccgtgccg atggtgatcg cgtgggattt tccggtgacc gagtttgggg
     2341 cgggaagaaa atggttgcgg cgctacacag agttttacgg gaagagcggc gcgaatgcct
     2401 gggagattgc gcgggacgga ttgcaaaatg cacgcaaatg gagcgaagca atcgatgctt
     2461 ggcaagcacc ttacctaaat gacgaatcga agccgctgtg gtatcgcggc atgctgttca
     2521 atgagctgta tgtatttgcg gacctcggtt ctttttgggg aaagcccgtg ggtgctgacg
     2581 cgaagacccc ctcgacgtat tcgttcatgg agtgcttcga ctatccgttt tacgagactc
     2641 tggatgtgcg gttttatggc tcgatgccgc tggtgaaatt ctggccggag attgacaagc
     2701 aggtgatgcg agagttttcc gagacggtgc cgaaacgttt gaacgaacag atgatgtgga
     2761 tctggaaaac tcaagaggcg cagaagctta catttcgcga gcgaaaaaca aagggagccg
     2821 ttccgcacga cctaggtgtg ccgaacgaag atccgttctt tcagatcaac cagttcagct
     2881 ggcaggacac taacggctgg aaggatctga attcaaaatt tgttttgatg gtgtatcgcg
     2941 attacgtttt gacgggaagc caggacgcgg tattcctgaa atacacgtgg ccgtcgatcc
     3001 aggaagcgct gacttatctc ggaaaattcg acaaggacgg cgacggtatt ccggacaacg
     3061 acggctaccc cgatcagacc tatgacacgt ggctggtacg cggagacagc gcatagtcgg
     3121 ggagtttgtg gctggcttcg ctgcgcgccg cggaagaaat cgccaagaca cttggtgata
     3181 cgcaggcgac ggcgaaatat cacgagcttt ttgcgaaggg gcagaaaagt ttcatcgcca
     3241 aactctggaa cggatcgtat ttccgctacg acacgggaag cgaataccgg gacaac
//
LOCUS       DASTPM010000251         6455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_95279, whole genome shotgun sequence.
ACCESSION   DASTPM010000251 DASTPM010000000
VERSION     DASTPM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95279"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFR42_04455"
     CDS             <1..333
                     /locus_tag="VFR42_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368435.1"
                     /translation="LSLLAVLPQAALWVAFRVAYPYFHTSFLLYLLVPVYLGAIVAAL
                     LPTSLSGRSFSNVHWTEIVASSTVAALLLIAIRLSSYSGDIFNSQAGDQVILAARVLA
                     GIARGMLT"
     gene            498..2849
                     /locus_tag="VFR42_04460"
     CDS             498..2849
                     /locus_tag="VFR42_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0368436.1"
                     /translation="MPAISSNQENSANAAPNAPVYWRVEGSLLELSALRQVGFFTWNA
                     QSFAQRWRRRLGMALMTLLRPFAYLVGRTFATRLLYSLLRGVTRDRLDLLGEEYFEYA
                     LKPSLSSEGVAALRRALDAGRPIVLFGESLDHVLRPLARHLGVDRFIANRMEFRDGVA
                     TGRLLAPVIHPRGPLAWIATSHADGRVPESQLLIELGLAAATQLHASVQPVLRSLPME
                     PPSVVSFDSAPSVAQLSVRRALAGKHVLLIGVTGFIGKVWMVDLLEQVPDIAKFTLLI
                     RRNRTTTAQRRFEKIVAESPTLDGLQKACGARLGDFLREKIDVVEGDVSLPGLGLDAD
                     VRARLARSLDLVVNSAGLTDFNPDLREALSSNVESAVHLIDFIRSTEHAALMHLSTCY
                     VVGMRDGRVAEELAENYNPAGIKNFDAEREITSLREMIQRIEERAESPELERALRRQA
                     LGRGMDETRVAAGELDGVVKRNRVRWARNKLVRVGMKRAQHLGWPNTYTFTKSLGESV
                     LARRGKDLPVAVVRPSIVESSTRSPFSGWNEGVNTSGPLSYLLGTNFRQLPTNAKKCL
                     DVIPVDMVCRGMSLIAAALIERKAARMYQLATSGVNPCDMGRSIELTGLAHRKHYRTQ
                     QGIEHWLKLKFETIPVSKQRYERLSIPMQKAVVSGINRFAEKLSMKKPPLAKAERDLG
                     RAEKLIELYEPFILHNEHVFSCENARLLSAALPEAERAIFSFDPEALDWWDYWINIHI
                     PALRRWCYPLMEGRPLESRPARDVGWGAQSAAAAAVAHSGENH"
     gene            2854..4011
                     /locus_tag="VFR42_04465"
     CDS             2854..4011
                     /locus_tag="VFR42_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0368437.1"
                     /translation="MAILLTGSTGYLGGYLATGLLRGYRDPLNLLVRARNQQEARERL
                     WQSLQLHMEFAEFSDRISACTRIFLGDLTNQQFGLPDDEYHALVQSTDSVIHCAASLN
                     RKSEKQCLNVNLRGTLEVIQLARRAQDHHGLRRFSHVSTVAVAGHRQDEVVQEDTAID
                     WARSDYDPYARTKKFCEHMVHQLLSEVDRRIFRPAIVLGDSTRPETTQFDMVQAFSVL
                     QALPVLPLKPTHRIDIVPANYVGKAIVTIHQKEKPSYRRYHLSSGTGSQTYRELTDAL
                     ASAASGSHPTYLPSLGKTFSGTVNWLSHKKSNLGRGASLLKVFWPYLVWNTVFDNARV
                     VEELGGEAPAKFSAYAYPLLKFSRDNKFRYPARPWPSQNASMTTASGAARA"
     gene            4008..5447
                     /locus_tag="VFR42_04470"
     CDS             4008..5447
                     /locus_tag="VFR42_04470"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEU0368438.1"
                     /translation="MTDFLLVAWVSMLIELTRLQWMLGGGKRWTPGEKLKLLFAGYNG
                     TRNTGSDVRVNEMLRQIRHVLGAENVDFSVMTQSFDRTKGYFEGSRQVHLPDVFPPFL
                     YRETRANHGVVACEGSMFKSKFANALTTMMIGSLGHASSENKLSVAYGAEAGHMESLI
                     EKMCARYVHDSLVITRNVESQSVLGKLGISTELGTDTAWTFEPHPDSYGRKALRDAGW
                     DEKTPVLVLCPIHPFVWPVKASLAKYIARVATGAYQESQYRTVYFHESGDEVERKFKH
                     YLDAFVSSTRAFTQKHRVFPILVAMERLDAVACRAIAAEIPGLPIFTSDDYHMYQLVS
                     ILRQCSYMVSSRYHGIVTCMPSLVPSAGVTMDERIRNLMRERGHQHLFLTVDDPDLGP
                     KMLQMMETLVVEADSVREGIGQTVVRNLKVMARMGVIFEDVLRRQYPEFPLRSGVRSW
                     EEYLPPLSEPLVRLAEKHDVAEAALAASK"
     gene            5453..>6455
                     /locus_tag="VFR42_04475"
     CDS             5453..>6455
                     /locus_tag="VFR42_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0368439.1"
                     /translation="MPNFLENIFARLQASPQRVVLREVHSEAADKVVSVSAGELLALV
                     ERARAWLRARQIAPGERCGLIGANSIHWIAADLALMAEGIVVVPLYHRQTAAELGGML
                     HDCQARLVLTGEAETATALSAWPQMPPRVALAEIFSDAAQPCLPGAPRERPDSQLLSI
                     IYTSGTSGEPKGVCLNISNLNHMTGCTTERLDQLMAASKKSQPDAIFLYAPMNFAASW
                     MLVLSALLRESVLTLSTDLNKLADEIRLAAPHYFLNVPTLLERIKRGVEDNLAKQPGP
                     LQSLYKKARDAWQRQRVGRRKPFDSITVALGRKLIFSKVTARFGPNLRALICGSAPLA
                     "
BASE COUNT         1229 a         2254 c         1657 g         1315 t
ORIGIN      
        1 ctaagcctcc tcgccgtcct gcctcaagct gcgctgtggg tagcctttcg agtggcctat
       61 ccgtattttc acaccagctt cctcttgtac ctgctggtac cggtctattt gggcgcaatc
      121 gtggcggctc tgctgccgac cagcctgtct ggccggtcgt tttcgaacgt ccattggacc
      181 gaaatcgtgg cgagttccac cgtagctgcc cttttgttga tcgcgattcg gctgagcagc
      241 tattcgggcg acattttcaa ttcacaagct ggcgaccagg tcattctggc cgcgcgcgtc
      301 cttgcaggaa ttgctcgtgg aatgttaact tagcgtttca cagtatctgt cgttccttcg
      361 ccggaaagcc aagaaatgtc aggggatgcc cgggacgccg gcggcggaat ccaggccaac
      421 aggatcgctt tgccttacga aacccatacc attgaattct cgcgcgccgc gcggcgccgt
      481 taggcattac gctcgccatg cctgctattt cttccaacca agaaaactcc gccaacgccg
      541 cgccgaatgc gccggtttac tggcgcgtgg aaggctcgct gctggagctg agtgccctgc
      601 gtcaggtcgg tttcttcacc tggaacgccc aaagcttcgc ccaaaggtgg cgccgccggt
      661 tgggaatggc gctgatgacc ctgctgcggc ctttcgccta cctggtgggc cgtaccttcg
      721 ctacgcggct gctatattcg ttgctgcgcg gggtcactcg cgatcgtctc gacctgctcg
      781 gcgaagaata ctttgaatac gcgcttaagc cgagtctaag cagcgaaggt gtggcggcat
      841 tgcgtcgcgc cctggatgcc ggacgaccca tcgtattgtt cggcgaatcg ctcgaccacg
      901 ttcttcggcc gctcgccagg cacttgggcg ttgatcgctt tatcgccaat cgcatggaat
      961 ttcgcgacgg cgtggccacc ggccgccttt tggctccggt catccatcct cgcggtcctc
     1021 ttgcctggat cgccaccagc cacgcggacg gccgcgttcc agaatcgcag ctcttgatcg
     1081 aactcgggct tgccgcagcc acgcaattgc acgcctccgt gcaacccgtg ctccgatcgc
     1141 ttcccatgga gccgccttcc gtcgtgtctt tcgatagcgc cccatccgtg gcgcagctca
     1201 gcgtgcgccg tgccctcgcc ggcaagcacg ttcttttgat cggtgtcacc ggcttcatcg
     1261 gcaaggtgtg gatggtggac ttgctcgagc aggttcccga catcgccaaa ttcaccttgt
     1321 tgatccggcg taaccgcacc acgacggctc agcgccgctt cgaaaagatc gtcgcggagt
     1381 cacccacact cgatggctta cagaaagcct gcggcgcgcg cctgggcgat tttctgcgcg
     1441 aaaaaatcga cgtcgtcgag ggcgacgtca gtctccctgg cttgggcttg gacgcagacg
     1501 ttcgcgctcg cctggcccgc tctctcgatt tggtcgtcaa cagcgccggt ctgacggatt
     1561 tcaatcctga cttgcgcgaa gcactttctt ctaacgttga atccgccgtg catctgattg
     1621 actttattcg ctctacggag catgccgccc tcatgcatct gtctacttgc tatgtcgttg
     1681 gcatgcgcga tggccgggtc gccgaggagc tggcggaaaa ctacaatccg gctggcatca
     1741 aaaactttga cgcggagcgt gaaatcactt cgctgcgcga gatgattcag cgcatcgaag
     1801 agcgcgcgga aagtcctgaa ctggagcgcg cattgcgccg ccaggccctt gggcgcggca
     1861 tggacgaaac tcgcgtcgct gccggcgaac tcgacggcgt tgtcaaacgc aaccgtgtcc
     1921 gttgggcgcg caacaagctc gtccgcgtcg gcatgaagcg cgcgcagcac ctcggctggc
     1981 ccaacaccta caccttcact aagagtttgg gcgaatccgt actcgcgcgc cgcggaaaag
     2041 acttgcccgt cgccgtggtt cgcccttcca tcgtcgaaag ctccacccgc agccccttca
     2101 gcggttggaa cgagggcgtc aacacttccg gcccactttc ctacttactg ggcacgaatt
     2161 tccgccagct tcccaccaac gccaaaaagt gtctcgacgt aattcccgtg gacatggtct
     2221 gccgcggcat gtcgctcata gccgccgcgc tcatcgaacg caaagccgcc cgcatgtatc
     2281 agctcgccac ttccggcgtg aatccctgcg acatgggccg cagcattgaa ctcaccggcc
     2341 tcgcgcatcg caagcattac cgcacgcagc agggaattga gcactggttg aaactgaaat
     2401 ttgaaaccat ccccgtttcc aagcagcgct acgaacgcct ctccattccc atgcagaagg
     2461 ccgtagtctc cggcatcaac cgcttcgctg aaaaactcag catgaaaaag cctcccctcg
     2521 ccaaggccga acgcgatcta ggccgcgccg aaaaacttat cgagctctac gaacctttca
     2581 tccttcacaa cgaacacgtc ttctcttgcg aaaatgctcg actgctctcc gccgcgcttc
     2641 ccgaagccga acgtgccatt ttcagtttcg accccgaagc gctcgactgg tgggactact
     2701 ggatcaacat ccacatcccc gctctgcgcc gctggtgcta tcccttgatg gaaggccgtc
     2761 cgctcgaatc gcgtcccgcg cgtgatgttg gctggggtgc gcaatcggcg gctgcggcag
     2821 ccgtggccca ctccggggag aatcactagc ccgatggcca ttttacttac cggctcaacc
     2881 ggatacctcg ggggctacct cgccacggga ctgcttcgcg gctatcgcga tccgttgaat
     2941 ctcctggtcc gcgcgaggaa tcagcaggaa gcccgcgaac gtctctggca atctcttcag
     3001 ctgcacatgg agtttgccga attcagcgac cgaatttctg cttgcacgcg cattttcctg
     3061 ggcgacctca ccaaccagca atttggcctg cccgacgacg aataccacgc cctcgtacaa
     3121 tccaccgatt ccgtcattca ttgcgcggcc tcgctcaatc gcaaatccga aaagcagtgc
     3181 ctcaacgtca atcttcgcgg cacgcttgaa gtcatccagc tcgcacgccg cgcccaggac
     3241 catcacggcc tccgccgctt ctcgcacgtc tcgactgtcg cggttgccgg acatcggcag
     3301 gacgaagtcg tccaggaaga caccgccatc gactgggctc gctccgacta cgacccctac
     3361 gcccgcacca agaaattctg cgaacacatg gtccaccaac tcctgtccga agtagaccgc
     3421 cgcatcttcc gccccgccat cgtcctcggt gacagcacgc gcccggaaac cacccaattc
     3481 gacatggtgc aggccttcag cgtcctgcag gctctgcccg tgctgcccct aaaacccacc
     3541 catcgcatcg acattgttcc cgccaactac gtcggcaaag ccatcgtcac cattcaccaa
     3601 aaagaaaagc cgtcttaccg gcgctaccat ctttcctccg gcaccggctc gcaaacctac
     3661 cgcgaactca ccgacgccct ggcctccgcc gcttccggct cgcatccaac ttaccttccg
     3721 tcactcggca agaccttttc cggcaccgtc aactggctct cccacaaaaa gagcaatttg
     3781 ggccgcggcg cttcgctcct caaagttttc tggccctacc ttgtgtggaa cacggtcttc
     3841 gacaacgccc gcgtggtcga agaactcggc ggcgaagcac cggccaagtt ttctgcctac
     3901 gcctacccgt tgctgaaatt cagccgcgac aataaattcc gctatcccgc caggccctgg
     3961 ccgtcgcaaa atgcctccat gaccaccgcc agcggagccg ctcgcgcatg accgattttc
     4021 tcctcgtcgc ctgggtcagc atgctgatcg aacttacccg cttgcaatgg atgcttggcg
     4081 gcggcaaaag gtggacgccc ggcgaaaaac tcaagctgct cttcgccggc tacaacggca
     4141 cccgcaatac cggctccgat gtccgcgtca acgaaatgct gcgccagatc cgccacgtcc
     4201 tcggcgctga aaacgtggac ttcagcgtga tgacgcaaag tttcgatcgc accaagggtt
     4261 actttgaagg ctcacgccag gtccacttgc ccgacgtctt cccgccgttc ctctatcgcg
     4321 aaacgcgcgc caatcatggt gtcgtggctt gcgaaggctc catgttcaag agcaaattcg
     4381 ccaacgccct cactaccatg atgatcggtt cgcttggcca cgcctcttca gagaacaaac
     4441 tctccgtcgc atacggcgcc gaggctggcc acatggagtc gctcatcgaa aaaatgtgcg
     4501 cgcgctacgt ccacgattcg ctggtcatca cccgcaacgt cgagtcgcaa tccgtcctcg
     4561 gcaaactcgg catctctacc gaactaggca ccgacacggc ttggaccttc gaaccccatc
     4621 ccgattccta cggccgcaaa gcgttgcgcg acgccggttg ggacgaaaaa actcccgtcc
     4681 tcgtgctttg cccgattcat cccttcgtct ggcccgtgaa agcatctctc gccaaataca
     4741 tcgcgcgcgt tgccactggc gcttaccagg aaagccagta ccgcaccgtc tatttccacg
     4801 agtccggcga cgaagtcgag cgcaagttca agcactacct tgacgccttt gtctcttcca
     4861 cgcgcgcgtt cacgcaaaag catcgcgtct tcccgatcct cgttgccatg gagcgcctcg
     4921 acgccgttgc ctgccgcgcc atcgccgctg aaattcctgg cctgcccatc ttcacttccg
     4981 acgattacca catgtaccag ctcgtctcga ttttgcggca atgctcttac atggtttcgt
     5041 cccgctatca cggcatcgtc acctgcatgc cctcgctcgt cccttccgcc ggcgtcacca
     5101 tggacgagcg catccgcaac ctcatgcgcg aacgcggcca ccagcatctc ttcctcacgg
     5161 tcgacgatcc cgatctcggc cctaaaatgc tgcaaatgat ggaaaccctc gtcgtcgagg
     5221 cagactccgt gcgcgaaggt atcggtcaga ccgtagtccg caacctcaag gtcatggctc
     5281 gcatgggcgt catctttgaa gacgttttac gccgtcaata tccggaattt cccctgcgca
     5341 gcggtgtacg ctcttgggaa gaatatcttc cgccgctcag cgagccgctt gtccgcctcg
     5401 ccgagaaaca cgatgtcgcc gaagcagccc tggccgcatc gaaatagctt tcatgcccaa
     5461 ttttctggaa aacatttttg cgcgcctgca agcctcgccg cagcgcgtcg tgcttcgcga
     5521 agtccatagc gaagccgccg ataaagtcgt ctccgttagt gcgggcgaac ttctggcgct
     5581 agtcgaacgg gcccgcgcgt ggctgcgtgc tcgacaaatc gcacccggcg aacgctgcgg
     5641 cctcattggc gccaattcga ttcactggat cgccgccgac ctcgcgctaa tggccgaagg
     5701 catcgtcgtc gtcccgctat accaccgcca gaccgcggct gaactcggtg gcatgctgca
     5761 cgactgccag gcgcggctcg tcctcaccgg cgaggcagaa acggccaccg cactttccgc
     5821 ctggccgcaa atgccaccac gcgtcgcact cgccgagatt ttctccgacg ccgctcaacc
     5881 ttgcctacca ggagcgcctc gcgagcgccc cgacagccag ctgctcagca tcatctacac
     5941 ctccggcact tccggcgaac caaaaggcgt ctgcctcaac atcagcaacc tcaatcacat
     6001 gaccggctgc accactgagc gcctcgacca gttgatggcc gcctccaaga aatcccagcc
     6061 tgacgccatc ttcctctacg cgcccatgaa cttcgccgcc tcctggatgc tggtgctctc
     6121 cgctctgctc cgcgaaagtg tcctcacgct ctctaccgac ctcaacaaac tcgccgacga
     6181 gattcgtctc gcagcaccgc attatttcct gaacgtcccc acgctcctcg agcgcatcaa
     6241 gcgcggcgtg gaagacaacc tcgccaagca gccgggcccc cttcagtctc tttacaaaaa
     6301 agcgcgagac gcatggcagc gccaacgcgt cggccgccgc aagcccttcg atagcatcac
     6361 cgtggcgctc ggccgcaaac tcatcttttc caaagtcacg gcgcgcttcg gtcccaacct
     6421 ccgcgcgctc atctgcggct ccgccccgct cgctc
//
LOCUS       DASTPM010000252         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_816240, whole genome shotgun sequence.
ACCESSION   DASTPM010000252 DASTPM010000000
VERSION     DASTPM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816240"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VFR42_04480"
     CDS             <1..1076
                     /locus_tag="VFR42_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0368440.1"
                     /translation="EMPRPFGTPASAARMTVSLPPPSPADALKQLARDIDSSIRFYET
                     YSGPFPVKQLNVSQIPGSFGQGWPGLLYLSTFSFLSRATQEQAGLSSSGQEHFHELVP
                     FHEVAHQWWGNVVAWSSYRDQWIDEALSSYLSVLFADSQKEPNRRLRVWLDRYRKRLL
                     DKGNSDTAPADIGALTLGNRLDSSKMPNGFEIVVYSKGTWIFHMIREMLREPKSKSPD
                     ARFIAFLLQLQQKYAYGSLSTQDLQRELEAVMTPAMAIEGRRSMEWFFADWVRGAGVP
                     HYKLEYTTKRSDKGFVVKGKLQQTGVPDSFVAPVPIYSVGAGYLGRVIAGGPETAFHF
                     VSQREPGKLVVDPQMTLLCVVDR"
     gene            complement(1080..1976)
                     /locus_tag="VFR42_04485"
     CDS             complement(1080..1976)
                     /locus_tag="VFR42_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368441.1"
                     /translation="MNTGKATAWGLLLLLPVGFAGVWRLQRKINTARDAMHREQDEVL
                     LRSPKLMKVLTGEYATFAADIYWTRAVQYYGNKRLGRDTDLQSLWPLLDLATTLDPNL
                     LPAYRFGATFLSEPEPRGAGRPDLAIQLLERGIQANPEYWRLNQDLGNVYYLELKDFP
                     KAGEAYLEGSRKPGSASWMKVMAARFLEKGDSRETAVMLWSEVYESTTDAALKENARI
                     NLQLLRTDEDIEHLNAMSEQFAGRAGRPPHSMHELAQAAKTGGEPADPLGYPYTIGPD
                     GKAEISEKSPLFKQKTVYRRPL"
BASE COUNT          406 a          680 c          568 g          481 t
ORIGIN      
        1 cggagatgcc gcggcctttt ggcactcccg cctcggcggc gcgcatgact gtgtcccttc
       61 ctcctcccag tccagccgac gcgctcaagc aattggctcg cgacatcgat tcctccattc
      121 gtttttacga aacctactct gggccctttc cggtgaagca attaaacgtt tcgcagatcc
      181 ccggcagctt cggccagggc tggcccggct tgctttatct atcgactttt tcttttttat
      241 cgagggctac tcaggaacag gcagggcttt cttctagcgg ccaggaacat tttcacgaac
      301 tggttccctt ccatgaagtc gcccatcagt ggtggggcaa tgtcgtcgcc tggtccagct
      361 atcgcgacca atggattgac gaggcgcttt ccagttatct gtcggtgctc tttgccgata
      421 gtcagaaaga gcccaaccgc aggttgcgcg tttggctgga tcgctaccgc aagcgtctgc
      481 tcgataaagg taattccgac acggctcccg ccgatatcgg cgccctaacg ctgggcaacc
      541 gcctcgattc ttcaaaaatg ccaaatggat tcgaaatcgt cgtctacagc aagggcacct
      601 ggatcttcca tatgattcgc gaaatgttgc gcgaacccaa atccaaaagc cctgacgcac
      661 ggttcatcgc tttccttctg cagctgcaac agaagtacgc ctatggctcg ctttcgacgc
      721 aagatttgca gcgtgaactc gaagcggtca tgacgcccgc catggccatc gaaggccgcc
      781 gctcgatgga atggttcttc gccgattggg tgcgcggcgc cggcgttccc cattacaagc
      841 tggaatacac caccaaacgc tccgacaaag gttttgtcgt aaagggaaag ctgcagcaga
      901 ccggcgtgcc ggattctttc gtagctcctg taccaatcta cagtgtgggt gccggctatc
      961 tcggccgcgt cattgcgggt ggaccggaaa cggcgtttca cttcgtttcg cagcgcgaac
     1021 ctggaaagct agtcgtcgat ccccaaatga cgctgctctg cgttgtcgat cgataaagtt
     1081 tatagcggcc gccgatacac ggttttctgt ttgaacagcg gactcttctc cgagatttcc
     1141 gccttgccgt ccggaccgat ggtgtacgga tagccgagcg gatcagcggg ctccccgccg
     1201 gtcttcgctg cctgggctag ttcgtgcatg ctgtgcggcg gccgcccggc gcgtccggca
     1261 aactgctccg acatcgcgtt caggtgttcg atgtcttcat ccgttcgcaa caactgcaaa
     1321 ttgattcgcg cattctcttt tagcgccgcg tcggtagtgg attcgtacac ttcgctccac
     1381 agcatcactg cagtctcgcg cgaatcgcct ttttccagaa accgcgccgc catcactttc
     1441 atccacgacg cggaacccgg tttccggctg ccttccagat acgcttcgcc ggctttcgga
     1501 aagtccttca attccaggta gtagacgttg cccaaatcct gattcagtcg ccagtattcc
     1561 gggttcgcct ggattcctct ttccagcagc tggatggcca ggtcgggccg ccccgccccc
     1621 cgaggctccg gctcgctcag aaacgtcgcg ccgaagcgat acgccggaag caggttcgga
     1681 tcgagcgtgg tggccaggtc cagcaacggc cacaaacttt gcaggtccgt gtccctgccc
     1741 agacgcttgt tgccgtaata ctgcaccgcg cgggtccaat aaatatccgc cgcgaaggtc
     1801 gcgtattcgc cggtgagcac tttcatcaac ttcggcgagc gcagcaatac ttcatcctgc
     1861 tcccgatgca tcgcgtcgcg cgcggtgttg atcttgcgct gcagccgcca cacaccggcg
     1921 aagcccaccg gcagcaacag cagcagcccc cacgccgtgg ccttgccggt attcatttca
     1981 ggtccttgcg cgagaagatt accgccgccg cgctcaacac gattccgcag taaagaatgg
     2041 tgtacagcgt gttgtgcaga accagcatcc ccgcaactcc gcgcccgtgt gccgccgcac
     2101 ccatcaggtc ataattgcca aagttcggta acacg
//
LOCUS       DASTPM010000253         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_787617, whole genome shotgun sequence.
ACCESSION   DASTPM010000253 DASTPM010000000
VERSION     DASTPM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787617"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VFR42_04490"
     CDS             complement(<1..669)
                     /locus_tag="VFR42_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEU0368442.1"
                     /translation="MQFSKYLAAAVLLIAPATMAEPAAGPKADKEKDVDRMENAGMVI
                     DEILNIPDDIPQDLLDKARCVVVLPSVLKAAFVVGGSYGRGVMVCRTGADFKGPWGAP
                     AMYALEGGSIGFQIGGQATDFVILVMNDRGASSLLKSKVKLGADAAVAAGPKGRDASA
                     NTDAYMRAEMLSYSRSRGVFAGISLEGSTLRPDNDANRNVYGKDATAAVIITESKFEA
                     PPEGR"
     gene            complement(722..937)
                     /locus_tag="VFR42_04495"
     CDS             complement(722..937)
                     /locus_tag="VFR42_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368443.1"
                     /translation="MKGATLLGFILVALGILALVYQGITYTTHKKVVDIGPIEATKEE
                     HKTIPLPPILGVIALVGGIVIIARDRK"
     gene            complement(1001..1165)
                     /locus_tag="VFR42_04500"
     CDS             complement(1001..1165)
                     /locus_tag="VFR42_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HEU0368444.1"
                     /translation="MLRWALIFFVVALLAAVLGFSGIAVAAAGVAKVLFFLFVVLLLI
                     TLLGHVARRQ"
     gene            1654..2319
                     /locus_tag="VFR42_04505"
     CDS             1654..2319
                     /locus_tag="VFR42_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368445.1"
                     /translation="MATGTLKVLVADDHEVVRKGLRTILEEQPGWEVSGEASDGREAV
                     DKVRSLKPDVTVVDVGMPGLNGLEATRQMLKNDPETKVLILTMHESDPLIREVLDAGA
                     RGYLLKSDAGKDLVSAVEAIRRNKTYFTARVAQMVLDGYLDKKPRTQVDTDVPASRLT
                     PRQREIVQLLAEGKSSKEVAVALNLSVKTAETHRANIMRRLNCHSVSELVRYAIRNNI
                     IDA"
     gene            2512..2928
                     /locus_tag="VFR42_04510"
     CDS             2512..2928
                     /locus_tag="VFR42_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368446.1"
                     /translation="MGALKILIADDHQSVRVLLRNILESVPNWKVCGEASNGTAVIAS
                     VRSLRPDVIVMDLMMPECNGLEATRELLRSEPEARVVLTTLHEFPSFAEEARRVGACG
                     CFFKAESGRQLIPAVRAAAERKPFFTSHDLEAGRPS"
     gene            3166..3348
                     /locus_tag="VFR42_04515"
                     /pseudo
     CDS             3166..3348
                     /locus_tag="VFR42_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
     gene            3468..3617
                     /locus_tag="VFR42_04520"
     CDS             3468..3617
                     /locus_tag="VFR42_04520"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEU0368447.1"
                     /translation="MLWTIFVILVVLWLLGMVSSYTLGGYIHLLLILAIAVVLIRVIQ
                     GRRPV"
     gene            complement(3635..>4015)
                     /locus_tag="VFR42_04525"
     CDS             complement(3635..>4015)
                     /locus_tag="VFR42_04525"
                     /inference="COORDINATES: protein motif:HMM:NF028495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEU0368448.1"
                     /translation="MLFQIRRRDGSVDPFSAGTFVDPRGNTRHLRSTEFQMTPTASAI
                     WTSPRTGAGYPVQWQVRVTCLDLTLAVSTPLPSQELVATSNIAPSYWEGAVLLQGKKN
                     SADIAGSGYLEMTGYAGPVSAREF"
BASE COUNT          908 a         1004 c         1312 g          791 t
ORIGIN      
        1 gcggccttcg ggaggagctt cgaacttgga ttcggtgatg atgacggctg cggtggcgtc
       61 tttgccatag acgttgcggt tggcgtcatt gtctgggcgc aaggtggaac cttcgagcga
      121 gatgccggcg aagacgccgc gcgaccgtga gtagctcaac atttcggcac gcatgtaggc
      181 atcggtgttc gcggaagcgt cacggccctt aggtccggca gcgacagcgg catcagcgcc
      241 gagcttcact ttgcttttga gaaggctgga ggctccgcga tcgttcatga ccagaatgac
      301 gaagtcggtg gcttggccgc cgatctggaa gccgatgcta cccccttcga gagcgtacat
      361 agccggcgct ccccatgggc ctttaaagtc cgcccccgtg cggcaaacca tcacaccgcg
      421 gccataactg ccgccgacga caaaagcggc tttcagcacg gatggcaaga cgacgacgca
      481 gcgggctttg tcgaggagat cctgtggaat gtcatcggga atattcagga tttcgtcgat
      541 gaccatgccg gcgttttcca tgcggtcaac gtctttttcc ttgtcagcct tggggccagc
      601 cgcgggctcc gccatggttg cgggcgcaat aaggagcaca gcggccgcga ggtattttga
      661 gaattgcatt tcttttcctc cctacgttgg tattgggacc ttcgtgcaaa tctggatgcg
      721 actatttgcg atcgcgggca atgatgacga tccctccgac aagagcgatg accccaagta
      781 tcggaggaag aggaatggtc ttgtgctctt ccttggtagc ttcaatgggg ccgatatcaa
      841 ccactttctt gtgggtggtg tacgtgatgc cctggtagac gagagcgagg atgccgagcg
      901 ctaccaggat gaaaccgagc aatgttgcgc ctttcatctt atggcctccg tgccattggg
      961 aaaattgcgc tgccggaatt gcgcggcgcg cctgaggtct ttattggcgt ctagcaacgt
     1021 gtcctaacag cgtgatgagc aacaggacca caaaaaggaa gaaaaggact ttagcgactc
     1081 cggcagcagc cacggcaata ccggagaagc cgagaaccgc tgccagcagc gctacaacga
     1141 aaaagatgag agcccagcgc agcatggcaa atgctccttt tacagccgct tcttaggaag
     1201 attgggtaca tccgtcacga agtgggaccg tttcctcctt ttgagatccg gaataagtga
     1261 tgactgggga gagtttgcag aagggcgggg gaatcgggcc atacagcgga cagagtatct
     1321 ttgcggaggg aatctgcgac ggaccgaaga actgtccgga aaggaagaga acaggaacat
     1381 gcgcggcaaa tatatcgtgc tgcgatagaa acatagttcg ctctgtcgag gcgaaaactg
     1441 tctaaccgag caaaaaggaa tggtatttcc tggggccgtg taaaaaatgc gaggccaggc
     1501 gtggatgtac cagtacccgg cgggcgggct aaagtctttc cctacagcga tctacgcaat
     1561 ggcgcgtgcc atccagattt tggccgattt tttgcgctga tgtggaatga ccctgcgaag
     1621 gaattaaggc attttattac ggaggaaata gcaatggcta cgggaactct taaggtgctg
     1681 gtggcggatg atcacgaagt ggtgcgcaaa ggtctccgca cgattctgga agaacagccc
     1741 ggctgggaag taagcggcga agccagcgac ggccgggaag cggtagacaa ggtgcgatcg
     1801 ctgaagccgg atgtgaccgt agtggacgta ggcatgccgg gtctcaacgg cctggaggcg
     1861 acgcgccaga tgttgaagaa cgatcccgag acgaaggtat tgatcttaac gatgcacgag
     1921 tccgatccat tgatccggga agtgctggac gccggcgcgc ggggatactt gctgaaatcg
     1981 gacgcgggaa aagatctggt gagcgcggtg gaagctatca ggcgcaataa gacttatttt
     2041 acggcgcgcg tggcgcagat ggtgctcgat gggtacctcg acaagaagcc gcgcacgcag
     2101 gtggacaccg acgtgccggc aagccggctt acgccacggc agcgcgagat cgtgcaattg
     2161 ctggccgagg gcaagagcag caaggaagtg gcggtggcgc tgaacttgag cgtgaagacg
     2221 gcggaaacgc accgcgcgaa catcatgcgg cggctgaatt gtcattcggt cagcgagttg
     2281 gtgcgttacg cgatccgcaa caacattatc gacgcttagg cagcaaacgt tgagaaccag
     2341 ggcttgcggg acgcgtacgg gaagtacgcg acgcgcaacg ccaggctaaa ctgctttacc
     2401 tggcaagaat cacttccata gttacagcga actaccgatt gcgggctcag cggtgaggtc
     2461 caattgtggg agtgtgccgc gcttggaagg ggccggcact ggccacgacc catgggagcc
     2521 ctaaaaatat tgatcgcaga cgatcatcaa tcggtgcgcg tgctgctgag aaacatcctg
     2581 gagtccgtgc cgaattggaa agtctgcggc gaagcttcca acggcacggc ggtgatcgct
     2641 tccgtacgga gtttgcgtcc ggacgtaatc gtaatggacc tgatgatgcc cgaatgcaac
     2701 ggcttggaag cgacacgaga gttgttgcgc tccgaaccgg aagcgcgcgt agtgctgaca
     2761 actctgcatg agtttccctc gtttgcggag gaagcgcgcc gggtgggcgc ctgcgggtgc
     2821 tttttcaagg cggagtccgg ccgacagctg attccggcag tacgggccgc cgccgagcgc
     2881 aagccgtttt ttacgtcgca cgatctggaa gccggccgcc cttcctgagg ccgccgaact
     2941 cctgttttca taaaataagt ccaagcgcgg ctacacgcgg ctgttgctgg tcctggttca
     3001 ctttctaaca gacagggcta cacggtattc gggaaagcag tggggcgttc gccgtattcc
     3061 ccgctttggg agcgcgtgtt tctctaggag tgaacggcga acgcgtggcc gcgcaaacga
     3121 agtgttcagc acgcggcgct gaaattccga cccgggagaa cgccgatgaa ttgggaacaa
     3181 gtagaaggga aatggaaaca gtacacggga aaagtccgcg agaagtgggg aaagctgacg
     3241 gacagcgact tggaaatgat caagggccag cggcagcagt tggtcggcaa gatccaggag
     3301 cgttacggaa ttgcgaaaga ggatgcggag cgccaggtga atgagttcac gagcggtctt
     3361 tttgaagacg acgctacgcg cgaagagcag cgagaggaca aggcgcgccg ggcaggacgc
     3421 taaagacttg aaggctcgat cgatctgacg gaagaaggag aacaggaatg ctttggacga
     3481 tctttgtgat tctcgtagtg ttgtggctgc tggggatggt gtcgtcctac acgctgggag
     3541 gatacatcca cctgttgctg attttggcga ttgcggtggt tctgatccgg gtgatccagg
     3601 ggcggagacc ggtctagcac gttcgaaacg ctcgtcaaaa ttcgcgcgcg gatacgggcc
     3661 cagcgtagcc ggtcatctcg agatagccgg agccagcgat atcagcggag ttcttctttc
     3721 cctgaagaag gacagctcct tcccagtagc tgggagcgat gttcgaggtc gcgaccagtt
     3781 cctgggaggg gagtggagtg ctgaccgcga gcgtgaggtc caggcaagtc acgcggacct
     3841 gccattgaac cgggtaacca gcgcctgtgc ggggacttgt ccagatggcg gaggctgttg
     3901 gcgtcatctg gaactcggta gaccgcaggt gcctggtgtt tccgcgcgga tcgacgaagg
     3961 ttccggcgga aaatggatcg actgaaccat ctctcctgcg aatttgaaag agcat
//
LOCUS       DASTPM010000254         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_902896, whole genome shotgun sequence.
ACCESSION   DASTPM010000254 DASTPM010000000
VERSION     DASTPM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902896"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFR42_04530"
     CDS             complement(<1..740)
                     /locus_tag="VFR42_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368449.1"
                     /translation="MRSTLDRIGLCALIAMMAAIFLSVAGPAALAQGGEPQYFAIRGA
                     KVVPVSGPPLEDATVVVARGVILAVGKDAAIPADAWVIEGKGLTIYPGLVDALTDVGL
                     ATAAPVPSPENAAAPGPARRAEELARGPEDRPNSTPWRSAADEVSLADKRIETWRSAG
                     FTTVVSSPRTGFFPGQAAVLDLNGERPGDLVVKAPVAIPISTKPASSFGRGFPDSLMG
                     VIAYEHQVLLDTDWLTKAEVAYEKNPRGT"
     gene            complement(907..2652)
                     /locus_tag="VFR42_04535"
     CDS             complement(907..2652)
                     /locus_tag="VFR42_04535"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0368450.1"
                     /translation="MNRTLRYTLIAVGVLVVLLIIVPFLIPVNRFRPTIEEKASAALG
                     RKVQVGDLSLSLLRGALTAKDLSIGDDPKFSPSPFLTAKSLTVGVEMMPLIFSKQLNV
                     TGITIDEPQVTLLKNPNGDWNYSSIGGNSGGGTPASAGKETKEAPANSPNASSSSSAP
                     DVSVKKLELKNGKINLGSTNSQQRSTYDHVNVTASDFSLHSKFPVSVTAELPGGGDLK
                     LDGNVGPIDKTDATLTPLDAKLHVSSMNLATTGILDPSLGLGGLVDLDTTLANQGGFA
                     QTNGTVKVSKALLVQGGSPAGVPMNVNYSTKYDLRKNSGVLNPSTVKIGSATAKLSGT
                     YASPGDATVVDLKLAGDGMSAKDLEAFLPAIGVHLPKGASLTGGTLSTNLNVKGPTNK
                     LVTDGTIGLFNANLAGFDLGSKMSAVSALTGLKTGKDLDIEKLTTNVHLAPSGLRADN
                     FLAVVPSLGNLAGGGTLDSKNNLDFKMAATLTGGAIGAIGGSGAGVGSVMGALGGSQG
                     SGCKGGTTIPFLVQGTASDPKFVPDVGGVAAGMLKSQLGCAGGSVANVGKAGGQTPAD
                     AVNALGGLLGKKKKP"
     gene            2844..2920
                     /locus_tag="VFR42_04540"
     tRNA            2844..2920
                     /locus_tag="VFR42_04540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2878..2880,aa:Arg,seq:cct)
BASE COUNT          627 a          954 c          867 g          667 t
ORIGIN      
        1 gtgccccttg gatttttttc ataggcgact tctgctttgg tcagccagtc ggtatccagg
       61 agcacctgat gctcgtacgc gataacgccc ataagcgaat cgggaaagcc tcggccaaaa
      121 ctgctcgccg gctttgtgga aatcggtatc gctacgggag ccttcaccac caaatcgcct
      181 ggtctctctc cattgagatc aaggacggcc gcctgccccg ggaaaaatcc tgttctagga
      241 gaggagacga cggtcgtaaa tcctgcgctg cgccacgtct cgatgcgctt atccgccagg
      301 ctgacttcat cggccgcact gcgccagggc gtgctgttcg gacgatcctc tggtccacgt
      361 gcgagttctt cggcgcgccg tgcgggtcct ggcgccgctg cattttccgg cgaaggcacg
      421 ggcgcagcag ttgccaggcc aacgtcggtg agcgcgtcca ccaggcccgg ataaatcgtg
      481 aggcctttac cttcgatgac ccacgcatcg gcgggaatcg ctgcgtcttt tccgaccgcg
      541 agaatgacac cgcgcgccac cacaacggtc gcatcctcca gtggcggccc agacaccggc
      601 accaccttgg cgccacgaat ggcgaaatat tgcggctcgc cgccttgcgc aagcgctgcg
      661 ggacccgcga ccgacaagaa aatcgccgcc atcatggcga tgagcgcaca caaaccaatg
      721 cgatccagag tagaacgcat gccagactcc ttatggaaag aaacgaaaag atcgaccgac
      781 cgcccgtcag tcgtttggac gcatgaggct actgggaagt ttcaagtcag tcaagccgat
      841 tggccgattg gccgcaacct tctaggcgtc gagaatgagc gcactcgatt gaacgcagaa
      901 aaatctctac ggctttttct ttttccctaa gagaccgcct agcgcgttaa cagcgtcagc
      961 cggagtttgt cccccggctt tgccaacatt agccaccgaa ccgccggcgc agcccagctg
     1021 cgatttcagc atgccagcgg cgacgccgcc gacgtccggc acaaatttgg gatcggaagc
     1081 agttccttga accaggaaag gaatcgttgt gccgcccttg caaccgctgc cttggctgcc
     1141 gcccaacgcg cccatcacgc tgccaactcc cgcgcccgaa ccgccaattg cgccgatggc
     1201 gccgccggtc agcgtcgcgg ccatcttgaa gtccagatta tttttcgagt ccagagttcc
     1261 accgccggct agatttccca agctaggcac gacggcaagg aaattgtcag cgcgcagccc
     1321 gctgggcgcc aggtgcacgt tagtggtgag cttctcgatg tccagatcct ttccggtctt
     1381 caacccggtc aacgcagata ccgccgacat tttcgatccc aggtcaaaac ccgccagatt
     1441 ggcgttgaac aggccgatgg tgccgtcggt caccagcttg ttggtgggcc ctttaacgtt
     1501 gaggttcgtg ctcaaagttc cgccggtgag cgaggctcct ttgggcaagt gcacgccaat
     1561 cgcgggcaga aacgcttcca aatctttggc tgacattcca tcgcccgcca gcttcaaatc
     1621 caccaccgtc gcatcccctg gcgacgcata cgttccgctc aacttggccg tcgccgaccc
     1681 gattttcacg gtcgacggat tcagtacacc ggaattcttc cgcaaatcat acttcgtgct
     1741 gtagttcaca ttcatcggca cgccggccgg acttccgccc tgcaccagca aagccttgga
     1801 taccttcacg gtgccattgg tctgcgcaaa accgccctgg tttgcgagcg tggtatccaa
     1861 atccaccagg ccgcccagtc ccagcgacgg atcgaggatg ccggtggttg ccaggttcat
     1921 cgaggaaaca tgcagcttgg cgtcgagcgg cgtcagcgtg gcgtccgtct tgtcgattgg
     1981 gccgacgttt ccgtccagct tcaggtcgcc gccgccgggc agttcggcgg tgacgcttac
     2041 cggaaacttg gagtggagag aaaaatcgga agccgtcacg ttcacgtggt cgtacgtgga
     2101 acgctgttga gaattcgtac tacccaagtt gatcttgccg tttttcaatt ccaatttctt
     2161 aactgagacg tcaggcgcac tcgaggaact cgacgcattt ggcgagttgg ctggcgcctc
     2221 tttcgtttcc ttgcccgcgc ttgccggtgt tccgccgccc gagtttccgc cgatagatga
     2281 atagttccag tcgccattgg gatttttcag cagcgttacc tgcggctcgt caatcgtgat
     2341 gccggtaacg ttcagctgct tcgagaaaat cagcggcatc atttccacgc cgacggtcag
     2401 cgatttcgcc gtcagaaacg gagagggact gaatttcggg tcgtcgccaa tggaaaggtc
     2461 ctttgcggtc agcgctccgc gcagcagcga caaactcaaa tcgcctacct ggaccttacg
     2521 tcccagcgcc gccgacgctt tttcttcaat cgttggccgg aaacgattca ccggaatcag
     2581 gaagggcaca atgatcagca aaaccaccag gacaccaacg gcaatcaggg tatagcgtag
     2641 ggtgcgattc atgatgacct ccgccttggc aggacccgtt cgcagggatg tttcgattgc
     2701 cctgttttcg aaagtgggtc ggaattgggg cggcgaaccg cataatacca tacggtcatt
     2761 aaataagaag catatggcct gcgcagcgcg gttggccaca atgaggcggc gttttgccgc
     2821 tataataaaa attgaccgac atcggggacg tagctcagat gcatagagcg aggcactcct
     2881 aacgcctagg tcgcgggttg gactcccgcc gtccccacca gtttttccgt tagtactttc
     2941 aaaccggcgc gctgtcgctc gcgttgacgc actcattcgc ctggcgtagc ctgattgcat
     3001 gagtcttgcg ccggaaaaat tgtcggccat tcgaggggct tctgtatttg tactggcgtt
     3061 tctgctgctc ctctcgcctg tttgcggcgc gttttgccag gcgcaaatgt gcgat
//
LOCUS       DASTPM010000255         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_931510, whole genome shotgun sequence.
ACCESSION   DASTPM010000255 DASTPM010000000
VERSION     DASTPM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931510"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(979..1698)
                     /locus_tag="VFR42_04545"
     CDS             complement(979..1698)
                     /locus_tag="VFR42_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU0368451.1"
                     /translation="MSFRERLEWLARMVFLIFILASAAFLSAITAMRIAIHGREVSMP
                     NLVGKGVSDASSDLRSRGLILRVADRVYSEQPINTVVRQTPSPGMLMKVSQQAHVVLS
                     LGQRALQIPPLEGDSLRVSRIELLRGGLQVGEVSALSLPDVPADTVVLQSPKPGASAA
                     TPRVDVLVSQGPREDSYVMPHLVGMNVADAVRRLESVNIKRKLNTLSAPQWPHGVVVD
                     QTPAAGTRIPISTTAELTVAE"
     gene            complement(1741..2454)
                     /locus_tag="VFR42_04550"
     CDS             complement(1741..2454)
                     /locus_tag="VFR42_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368452.1"
                     /translation="MLYSPLLPVHIAGGITGILFGAAALIYRKGSARHALAGKVFVAA
                     MLTMASAAVYLAVLKNQPGNIGGGIFTFYLILTAWLTARRREGETRTYDWAAVVIPVA
                     LGVLTWMNGIRVLRSGATSENGVPVGMSLFMGTVMLLAAAGDVRMLLRGGLYGAGRVV
                     RHLWRMCFGLFIATGSFFLGQQQVFPKWLRGSPALFVPALLPLVLLIFWVLRVRFGKI
                     YKGSGPRKAPVLVQKETAA"
BASE COUNT          640 a          901 c          741 g          484 t
ORIGIN      
        1 gatggtcgga ttctttaacc tcgttttcga cgctcaaatg cccgtcgaaa acctcttcgc
       61 agacaagaaa aacaacgccg gcatcgctaa aaactgacca cgtgtagcgg ccgccttctg
      121 aggcggggcc tcttgcgact ctgcttacga ttcgcctgtg caaaactccc accactttgc
      181 acagccgtcc acagatttgc acaagtgcga attacgaatt ccgttgacgt gaattcgaaa
      241 tgaaactatc ttgcgcacca gattgaaccg ggaagcaaag aactttacgg atcaatttat
      301 aaggcgcagc aacctaacgg tatgcagcgc cgcgagattg aactgcaaag ctcgaatcct
      361 tgacggtttc gtatcggtcg agcggcaagt ctaaacagcg agccgaactg ccggacggaa
      421 tcgggaagcc caaagacttc taggttcggt gaagcaggcg cagcaaactt aaccgtatgc
      481 agcgccgcga aatcgaaaac cggaagggaa taagcttcac gctctactcg gacggtcatt
      541 cgcaggaacg cgcaagcggg acagcggacg catcgccaga atggaatacg caaggcccga
      601 aactttacgg ctcagtagag cacgcaacgc gccgcaaggc gtaaccgcgg acccaggcca
      661 gaggcggacg ccgcaaggcg gaagcccaag gccaaaagtc caaggtgcag ttgcgatgcg
      721 aaattgaact ggcaaagcat tcgaagcccg atcgttttcg gacgaaggag tcggcaagcc
      781 gcaaggcccg ccgcaaccga cggaagggaa cgggaaggtc agaaacttta cggatcaatt
      841 agagggcgca acacccgaag ggccaaaagc ccagcggaag ttgcgccctc gatttttttc
      901 gcctcgatgt tctcaagctc gccgttcttc tcttctttcc gctcaattct tttcgaagca
      961 aaatctttcg cagtaacttt actccgccac cgtcaactcc gccgtagttg aaattggaat
     1021 tcgcgttccc gccgcgggtg tctgatcgac caccaccccg tgcggccact gcggcgcgct
     1081 cagcgtattc agcttgcgtt tgatgttcac gctctcgagc cgtcgcaccg catccgccac
     1141 attcatcccg accagatgcg gcatcacgta cgaatcttcg cgcggcccct gcgataccag
     1201 cacgtccacg cgcggcgtcg cggcgctagc acccggcttg ggactttgca gcaccacggt
     1261 atccgccgga acgtccggca acgacagtgc cgacacttcg ccgacctgca atcctccgcg
     1321 caacaactca atccgcgaca cccgcagcga atctccctcc agcggaggaa tctgcagcgc
     1381 gcgctgtccg aggctcagca ccacgtgcgc ctgctgcgaa actttcatca gcattcccgg
     1441 cgaaggcgtc tgccgcacca ccgtattgat cggctgctcg ctatacacgc gatcggcgac
     1501 tcgcagaatc agcccgcgcg atcgcaagtc gctactcgcg tcgctgacgc ctttgcccac
     1561 cagattaggc atgctcactt cgcgcccgtg aatcgcaatg cgcatcgccg taatcgcgct
     1621 caaaaaggcg gccgacgcca gaataaaaat cagaaacacc atgcgggcca gccattccag
     1681 gcgttcgcgg aagctcatgg cagtagttta cggcgctgcg gctcgtcgcg gtttgcgaat
     1741 ttacgccgcg gtttcctttt gcaccagcac cggcgccttg cgtggtcccg atcctttata
     1801 gattttccca aaccgcacgc gcagcaccca gaaaataagc agcaccagcg gcagcaacgc
     1861 cggcacaaaa agcgctggcg atccgcgcag ccacttggga aaaacctgct gctgccccaa
     1921 aaagaatgag cccgtggcga tgaacagccc aaagcacatc cgccacaaat gccgaaccac
     1981 gcgcccggcg ccataaaggc ccccacgcaa caacatgcgc acgtctcccg ccgcggccag
     2041 cagcatcacc gtccccatga ataagctcat tccgaccggt actccgttct ccgaagttgc
     2101 cccgctgcgc agtactcgta tgccgttcat ccaggtcagg acgccgagtg ccaccgggat
     2161 caccacagcc gcccagtcgt acgtgcgcgt ttcgccttcg cgccgcctcg cggtcagcca
     2221 cgccgtcagg atcaggtaga aggtgaaaat gcccccgcca atattgcccg gctggttttt
     2281 cagcacggcc aggtacaccg cggccgacgc catggtcagc atagccgcca caaacacttt
     2341 tccggcaaga gcatgccgcg ccgatccctt tcgatagatc agcgccgcgg cgccaaacag
     2401 aattccggtg attccgccgg caatatggac gggcagcagg ggagagtaaa gcattggggc
     2461 acttccctgc agttaggtac ggggaacctg ctaggattgt ttcacagaca cgccggaaaa
     2521 cgcgcccttc cgcgctcctg gggcgagccg ggctccaggg gcgccactac aagcacgtca
     2581 atagtcgcgc cagggcttcc agacgggagt gctgaggtag cgaatctcgc tcggccggga
     2641 cagacgccgc ctcaaggccg caagaactcg cgcaaatctg ttcttgagca gtacggcaaa
     2701 cataatgcac gaactatgga cgccggctcg gccgctgtca atcgcccgtt ccggggttca
     2761 ccctca
//
LOCUS       DASTPM010000256         4083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1392890, whole genome shotgun sequence.
ACCESSION   DASTPM010000256 DASTPM010000000
VERSION     DASTPM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392890"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFR42_04555"
     CDS             <1..544
                     /locus_tag="VFR42_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368453.1"
                     /translation="ALHQQEDLGNALKSYQQAVKVDPKYADAQNNIGTIWYQRKKFGK
                     AIRSYQKAIKLRTDMAVLYSNLGYAYFGERKYEESIGAFRQALAVDPQCFEHSSSRSG
                     SLLQDRSVSDRGRFYFLLAKSFAQAGNIERSLVYLRKAKEEGYGAMNEVKTDPAFAAM
                     VKLPETQEILLPKTTDPTQP"
     gene            655..2361
                     /locus_tag="VFR42_04560"
     CDS             655..2361
                     /locus_tag="VFR42_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368454.1"
                     /translation="MKWKLVRMLCLSGYAAVCVASLSSQTTVPGRPVVPASQGIFLVF
                     PFENNGANARLDWLCEGLEELTIQRLSAAGQKVFTHAGRTTELDRYGLPSNAQFSHAT
                     MLRIGADLDADFVVFGKFSSDGKSLTIEARALRVNPTKLSEVVRESGTLDSLMELNSK
                     VVWKLLAANDKGLPINLTEFSQLQRPLRLDAFEHYIRGLLATDDDGRVRELRESARLD
                     PDWPSPAFALGQAYFSRRDCESALPWLAKVPAGNSLSAEAMFTSGVCRLLMNQPDKAE
                     ETFGALQNNLKNDLVAGAELPEVLNNLALARARRGNTTAAASGLLRALDLDPDEDDYP
                     FNLGLLYLRGNDATTAAKYFREASDREPENPEDRAMLVYALEKTGNKTEAAQERNSAI
                     ETLALNALPPVKPESFARLDRVSTDLDVTTLQLQIASRDSNGGTTGSASSSAVSSPTS
                     LVRKGRQELAAGRVDAAEADFRAALAAAPNDPAAHRGMAEVLRRRSKRAEAVQELQAA
                     LSERDSAVDRTTLARIYLEQKKPELAKSELQKALKIAPNYSEARQLLDVLQNGNGKAA
                     TP"
     gene            2358..3728
                     /locus_tag="VFR42_04565"
     CDS             2358..3728
                     /locus_tag="VFR42_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEU0368455.1"
                     /translation="MKPLKKCHATARVSIFASVAMWALVLLLPAASVSDENSTRPLVM
                     DLNLDGEVEPVLATYIDEGLAEAARRNASLVLITMDTPGGLSDSMKDIIQHVLASPVP
                     VAVYVGPTGARGASAGFFILMSADVAAMAPGTRTGAASPVIAIGGFTIGVDEVLRRKI
                     MNDAMAFLRSYTEKRGRNAKLAETAVTDARAFTEKEALEGNLIELVVSSREELLRDLD
                     GRTVARFDGSKTTLKLTNYQRAAFELSPRQKFLSRIVEPDMFFLLLILGTLGLYAEFT
                     HPGAVIPGVVGAICSVLALYAMHLLPVNIAGVLLILTAVTLFVLEAKFTSHGVLLLGG
                     VIAMVFGAIFLIRSPLTTGGVSLGMAVAVTLPFAAISVFLMRLVLRSRRWKPAAGAEE
                     LLSEQGVAVSGLKRGEEGMIRIHGETWRAVSSEDVAVGAPVRVRRIEGLKLHVVPASE
                     AAGKSS"
     gene            3766..>4083
                     /locus_tag="VFR42_04570"
     CDS             3766..>4083
                     /locus_tag="VFR42_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU0368456.1"
                     /translation="MNIVLLGIAFVVFMVLIWIRNSIYIIKEWERGVVLRLGKLLPQA
                     KSSGINFVFYPIETLQRVSLRLETLDVPPQDIITHDNVSVKVNAVCYFRVVDANLAIS
                     QVAN"
BASE COUNT          847 a         1031 c         1358 g          847 t
ORIGIN      
        1 cgctttgcat cagcaagaag atctgggcaa cgccctgaag tcgtatcagc aggccgtgaa
       61 ggtggatccg aagtacgccg acgcgcagaa caacattggg acgatttggt atcagcggaa
      121 aaaattcggg aaggcgattc gttcctacca aaaggcgatc aagctccgca ccgatatggc
      181 cgtgctctac agcaacctgg gctatgcgta tttcggcgag aggaagtacg aagagtcgat
      241 tggcgcgttc cggcaggcgc tggcggtcga tccgcagtgc tttgagcaca gttcgtcgcg
      301 ttccggttcg ctgttgcagg accgctcggt tagcgaccgc ggcagattct atttcctgct
      361 ggcgaagtca ttcgcgcaag ccggcaatat cgaaagaagt ctggtctatt tgcgcaaagc
      421 caaagaagaa ggctatggcg cgatgaacga agtgaagacc gacccggcgt ttgcggccat
      481 ggtgaagctg ccggaaacgc aagagatttt gctgccaaag acgaccgatc ccacgcaacc
      541 ctaaacaaag ttcctggata acaagcggat gcccgccgat ccgtgttgct gcaggtgtgg
      601 caacatgctt cgttcttgcg cgttaggcga ttcgcgctct gttacaattt gcgcatgaaa
      661 tggaagctgg tgaggatgct atgcctgagc ggttatgcgg cggtctgtgt agcgtcgctt
      721 tccagccaga cgacggttcc cggacgtccc gtggtgcccg cctcgcaggg aattttcctc
      781 gtttttcctt ttgaaaataa tggcgcgaat gcccgtctgg actggctttg cgaagggctg
      841 gaagaattga cgattcagcg tttgtccgcg gcagggcaga aggtttttac gcatgcaggc
      901 cggacgacgg aactggatcg ttatggactt ccttccaacg cgcagttcag ccacgcgacc
      961 atgctgcgga ttggtgcgga cctggacgcc gactttgtgg tcttcgggaa attctcttcg
     1021 gacgggaaat cgctgaccat cgaagcgcgt gccttgcgtg tgaatccgac gaaattgtcc
     1081 gaagttgttc gcgagagcgg aacgctggat tcgctgatgg agctgaacag caaagtagtg
     1141 tggaagctgc tggcagcgaa cgacaagggg ttaccgatca acctgaccga attttcgcaa
     1201 ttgcagcgcc cgctgcgatt ggacgcattc gaacattaca tccgaggctt gttggccacc
     1261 gatgatgacg gcagggtgcg tgaattgcgc gaatcggcgc ggctagatcc ggattggccg
     1321 tcgcccgcgt ttgcgctggg gcaggcatat ttttcgcggc gcgattgcga gtcggcgctg
     1381 ccgtggctgg cgaaggtgcc ggcggggaat tcgctttcag cggaagcgat gttcaccagc
     1441 ggggtttgcc gcctgttgat gaaccagcca gataaggccg aggaaacgtt cggtgcgctg
     1501 caaaataatc tgaagaacga cctggttgcc ggcgcggaac tgcccgaagt gctgaacaat
     1561 ctggcgctgg cgcgagcgcg ccggggaaat acgacggctg cggcttcggg tttgctgcgg
     1621 gcgctggacc tcgacccgga cgaagacgac tatccgttta acctcggctt gttgtacctc
     1681 cgcggaaacg atgcgacgac ggcggcaaaa tattttcgcg aagcctcgga tcgcgagccg
     1741 gaaaaccccg aggatcgcgc gatgctggtt tacgcgctgg agaaaaccgg caacaagacc
     1801 gaggccgctc aggagcgcaa ttcggcgata gagacgcttg cgctgaatgc gcttcctccg
     1861 gtgaagccgg aaagctttgc ccggctggac cgtgtttcga cggacttgga tgtaaccacc
     1921 ttgcagctgc agatcgcttc gcgcgatagc aatggtggca cgaccgggag tgctagttcc
     1981 tcggcagtca gttcaccgac gtcactcgtc cgtaagggcc gacaggaatt ggcggcagga
     2041 cgcgtggatg cggcagaagc ggattttcgc gccgccctgg cggcggcgcc aaacgatccc
     2101 gccgcgcacc ggggtatggc cgaagtgttg cgacgcagat cgaagcgagc ggaagccgtt
     2161 caggaattgc aggcggcgct ctcggaacgg gattcagcgg tggatcgcac cactttggca
     2221 cgaatctatc tggaacaaaa gaagccggag ctggccaaga gcgaattgca gaaagcactg
     2281 aagatcgcgc cgaattactc ggaagcgcgg cagttactgg acgttttgca gaacgggaac
     2341 ggaaaagcgg cgacaccatg aagccgttga aaaaatgcca cgcgacggct cgcgtatcta
     2401 ttttcgcgag tgtagcgatg tgggcgctgg tgttgttact tcctgcagcg agcgtctcag
     2461 acgaaaattc gacacggcca ctggtgatgg acctcaacct ggatggcgag gttgagccgg
     2521 ttctggccac gtacattgac gaagggttgg ctgaagcggc acggcgcaac gcctcgctcg
     2581 tactgatcac aatggacacg cccggcggac tgagcgattc catgaaggac atcattcagc
     2641 atgtgctggc gtcgccagtg ccggtggctg tgtatgttgg gccgaccgga gcgcgcggcg
     2701 cttcggcagg atttttcatc ttaatgagcg cggatgtggc ggcgatggca ccgggcacgc
     2761 ggacgggcgc cgcttcaccg gtgattgcga tcggcggatt cacgatcggc gtggacgaag
     2821 tgttgcgccg aaaaataatg aacgacgcaa tggcatttct ccgcagctac acggagaagc
     2881 gcgggcgcaa cgcgaaacta gcggaaacgg cggtgacgga tgcgcgggcg ttcacggaga
     2941 aagaagcgct ggaggggaac ctgatcgagc tggtggtgtc ctcacgggaa gagttgctgc
     3001 gcgacctgga tggccggacg gtggcgcggt tcgacggcag caagacgacg ctaaagctga
     3061 cgaactatca gcgggccgca ttcgagttgt cgccgcggca gaagtttcta tcgcgcatcg
     3121 tcgagccgga tatgtttttt ctcctgctga ttctgggtac gctcggcctg tatgcagagt
     3181 ttacgcatcc aggcgcggtg attcccggtg tcgtcggggc gatctgttcg gtgctggcgc
     3241 tgtatgccat gcatctctta ccagtgaata tcgccggggt gttgctgatc ctgacagcgg
     3301 tcacgctgtt tgtgttggag gcgaagttca cgagccatgg agtcttattg ctgggtggtg
     3361 tgatcgcgat ggtgttcggg gcgatttttc tgattcggtc accgctgaca acgggtggag
     3421 taagcctggg gatggcggtt gccgtgacgc ttccgttcgc cgccatcagc gtgtttttaa
     3481 tgaggctggt gctgcgctca cgcagatgga aaccggcggc gggagcggag gaactgctga
     3541 gcgaacaggg agtcgcggtt tccggattga agcgtgggga agagggcatg atccggattc
     3601 atggcgagac ctggcgggcg gtgtcatcgg aggacgtggc tgtgggagcg ccagtgcgcg
     3661 tacgacgcat cgagggttta aaactgcatg tcgtgcctgc atccgaggca gcgggaaaga
     3721 gttcttgaag gcgtagtgca tgaaaagaag gcgggggcag cgtccatgaa tatcgtactg
     3781 ttggggattg ctttcgtagt gtttatggtt ctgatttgga ttcgcaattc gatctacatc
     3841 atcaaggaat gggaacgcgg cgtggtgctg cggttgggaa aacttttgcc gcaggcgaaa
     3901 tccagcggca tcaattttgt gttctacccg attgaaacgt tgcagcgggt cagtctgcgg
     3961 ttagagacgc tggacgtgcc gccgcaggac atcatcacgc acgataacgt ttccgtaaag
     4021 gtgaacgcgg tatgttactt ccgggtagta gacgcgaacc tggcgatttc ccaggtggct
     4081 aac
//
LOCUS       DASTPM010000257         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_729824, whole genome shotgun sequence.
ACCESSION   DASTPM010000257 DASTPM010000000
VERSION     DASTPM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729824"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFR42_04575"
     CDS             <1..836
                     /locus_tag="VFR42_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368457.1"
                     /translation="SPAAFQAGQSNEPAQVSTEIKKEKKRSFFHAWLALADQVQATQP
                     DWLSPLATTSGRLKQEFRYDIWDQPTIGGNRSYQFGGNKGLEFITSSRTQILIGVPTY
                     TLLSPSGPPAGFGDLPLMLKVRISSAERTEGNYLVTFLLAATAPTGSHRYGAGEAVVT
                     PTMAFGKGWRRFDVQSTLGVNLPAAGTAKLGRQVLWNTAFQYQAAWKLWPELEVNSTF
                     YTTGKYAGETQVFLTPGLGFGRAHLAGRFRFSSAVGVQIAATPFKTYNHRWMLSTRLS
                     F"
     gene            1026..1982
                     /locus_tag="VFR42_04580"
     CDS             1026..1982
                     /locus_tag="VFR42_04580"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368458.1"
                     /translation="MILHQLRHDINRALTPRVPKPMQNQQQILKAIAAKAQELGRAPT
                     LLEFVTDARIPKNWVFRLFPRWNEAVQAAGLQPFRLYRRPEDHELLIDWGQTVRKLRV
                     VPSARAYDLHGTYYPATLEKRFGSWPVIPQVFRDFAKGQREWADVLALLREAEQSAAA
                     ALSQQTGSRHTIVGARDTRARCASSRASNPSKTSSHPPLKQRPTYGEPLDFPTLRHEP
                     MNEQGVVLLFGMLAKDLGYVIESVQTGFPDCEAKRQIGPSRWQRVNLEFEYESRNFHE
                     HGHPLTGCDVIVCWRHNWPDCPANIEVLELSTRIHSFCSAAR"
     gene            complement(2128..>2424)
                     /locus_tag="VFR42_04585"
     CDS             complement(2128..>2424)
                     /locus_tag="VFR42_04585"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU0368459.1"
                     /translation="EYVNPWKIPTFDLNGPLCFYMVAKEHVVFGFMRGAKLRDPEKLL
                     EGTGKYLRHVKLRSAAEVRQPGVRNLVKQAAALNRKKPVTGTKVQMKKRSADRK"
BASE COUNT          515 a          804 c          633 g          472 t
ORIGIN      
        1 agagtcccgc agcatttcag gccggccaaa gcaatgaacc agcccaggtg tccactgaga
       61 taaagaaaga gaaaaaacga agtttctttc acgcctggct cgccctggca gatcaagtgc
      121 aagcgacaca accagactgg ctttctccct tggcaaccac atcggggcgc ctgaaacagg
      181 aattccgcta cgatatttgg gatcaaccaa ccatcggcgg gaacaggagt taccaatttg
      241 gtggaaacaa gggcctggag ttcatcacca gctcacgcac gcagatcctg atcggtgtgc
      301 cgacctatac gctgctatcc ccgagcggtc cgcctgcggg attcggtgat ctccccttga
      361 tgttgaaagt ccgcatctct tcggcggagc gcacagaagg caattatttg gtgacgttct
      421 tattggcggc aaccgcgccg acgggttcgc atcgctacgg cgctggcgaa gccgttgtca
      481 cgccgacaat ggcgttcggc aaaggctgga gacgatttga cgtgcagtcg accttgggcg
      541 tcaatctgcc tgcagcaggc acggccaagt tgggcagaca agtgctgtgg aacaccgcgt
      601 tccaatatca ggcggcgtgg aaactctggc ccgagctgga agtcaactcc acgttctaca
      661 ccaccggaaa atatgccggc gaaacgcaag tgttcctgac ccccggactt ggctttgggc
      721 gcgcgcatct cgctggacga tttcggttct cttccgcggt gggcgtgcaa atcgcggcga
      781 ccccgttcaa gacctacaat catcgctgga tgctttcgac gcgcctctcg ttctgagcac
      841 aactctgcac gacaccgctg atggcgttcg ctcgagtccc gcctaaatcg gcaacccgga
      901 agttatcagt ttccccgtag ctgccacctt caggtgggcc agcttgcgcg ccagcgcaag
      961 ctctccctag cgcaaaaata ttcatcgaag tgtgattcaa gaaggtctca gcgccataag
     1021 gcgtcttgat cctgcaccaa ctccggcacg acataaatcg cgccctgaca ccacgtgtcc
     1081 ccaagcccat gcaaaaccaa cagcaaatcc taaaagccat tgccgccaag gcccaggaac
     1141 tcggccgcgc acccactctc ctggaattcg tcaccgacgc gcgcattccc aagaattggg
     1201 tcttccgtct cttccctcga tggaatgagg ccgtccaagc cgcgggtctc cagccgttca
     1261 ggctgtatcg ccgcccggag gaccacgaac tcctgatcga ctggggccaa acggtccgca
     1321 aacttcgcgt cgtgccttct gcgcgcgcct acgatctgca cggaacgtat tacccggcca
     1381 cgctggaaaa acgcttcggc tcctggcccg tcatcccgca agtctttcgc gatttcgcca
     1441 aaggccagcg cgagtgggcc gacgtcctgg ccctccttcg cgaagccgag cagtccgctg
     1501 cagccgcatt aagccagcaa acaggttccc gccacacaat cgtaggggca cgggacaccc
     1561 gtgcccgctg cgcaagctcc cgcgcgtcaa atccatccaa gacatcttca cacccgcccc
     1621 taaaacaacg ccccacctac ggtgagcccc tggacttccc gaccctgcgc cacgaaccga
     1681 tgaacgaaca gggcgtcgtt ctcctgtttg gaatgctcgc gaaagacttg ggctacgtga
     1741 tcgaatccgt gcaaaccggt ttccccgatt gcgaggccaa gcgccagatc gggcccagcc
     1801 gctggcagcg cgtgaacctc gaatttgaat acgagagccg caatttccac gaacacggcc
     1861 acccgttaac cggctgcgac gtaatcgtct gctggcgcca caactggccc gactgccccg
     1921 ccaacatcga ggtcctcgaa ctctccaccc gcattcactc gttttgcagc gccgccaggt
     1981 gaccgccaaa gaatgtggag tgcggcggac ttgccgccgc ttttacagcg taatgtactt
     2041 aggctgcctg atctgatcat cgagcagtag gccccgacac gcagaaagcg gcgcccgcct
     2101 gcggcaggca agcctcccgc actccaatta tttccgatcc gcgctgcgtt tcttcatctg
     2161 aaccttggtg ccggtgaccg gctttttgcg gttcaaggcg gccgcctgtt tgacgaggtt
     2221 gcgcactccc ggttgccgca cctccgccgc gctgcgcagc tttacgtgcc gcagatattt
     2281 tcccgtgcct tccagcaact tttccgggtc gcgcaatttc gctccgcgca taaatccaaa
     2341 caccacgtgt tctttcgcca ccatgtaaaa acacaacggc ccattcagat cgaacgtggg
     2401 aatcttccac ggattgacgt actc
//
LOCUS       DASTPM010000258         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_528225, whole genome shotgun sequence.
ACCESSION   DASTPM010000258 DASTPM010000000
VERSION     DASTPM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528225"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /gene="ruvA"
                     /locus_tag="VFR42_04590"
     CDS             <1..455
                     /gene="ruvA"
                     /locus_tag="VFR42_04590"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU0368460.1"
                     /translation="DTLALYGFLSSREKHFFELLLGASGVGPSLALKILSGMTVEELV
                     PAIRGGDLVRLTKIPGVGRKTAERMVVELKDKLEAVTVAEEKQPASKAGVEADVVSAL
                     TNLGYDGRQAESAVAEGKRAAGTASFEKLLKAALQALAGPKETSARVP"
     gene            481..1551
                     /gene="ruvB"
                     /locus_tag="VFR42_04595"
     CDS             481..1551
                     /gene="ruvB"
                     /locus_tag="VFR42_04595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0368461.1"
                     /translation="MADGTAKRMMQREHDMRERPERIVSGAAYDEDARLDASVRPKTL
                     DEYIGQKRVKENISIALEAARNRGEALDHVLLYGPPGLGKTTLAQIIANELNVPIKTS
                     AGPLLERKGDLTAILTSLETREVFFLDEIHRLQAAVEEVLYPAMEDFRVDLVIGQGPG
                     ARIHPFHLNHFTLVGATTRAGLITAPLRSRFGIVHRLDFYEPEDLLIILKRSAKILHI
                     EMEEGGAEEIARRCRGTPRIANRLLRRARDFAEVRADGKITRGVAQEALSMLGVDGKG
                     LDEVDRNLMLALLDKYGGGPVGVGTLAAALSEEEDAIEEMYEPYLMQIGMIDRTPRGR
                     VATPRAYEYFGRELPRRQPRLF"
BASE COUNT          455 a          441 c          740 g          367 t
ORIGIN      
        1 aagatacgct ggcgctttac ggatttcttt cttcgcgcga aaagcatttt tttgagctgc
       61 tgctgggagc gtcgggagtc ggcccgtcgc tggcgctgaa aattttgtcg ggcatgacag
      121 tagaggaatt ggtgccggcg attcgcggtg gcgatctagt gcggctgacg aaaattcccg
      181 gcgtggggcg aaagaccgcg gagcggatgg tagtggagtt gaaggacaag ctggaagcgg
      241 tgacggtagc agaagaaaag cagcccgcgt cgaaggcggg agtggaagcg gacgtcgttt
      301 cggcgctgac gaatctgggt tacgacgggc ggcaagcgga gagcgccgtg gcggaaggaa
      361 agcgcgcggc ggggacagcg agcttcgaga aattgttgaa ggcggcgttg caggcgttgg
      421 ctggaccgaa ggaaacgtcc gcgagagttc cgtaagcgga tgaacggact tgagtgaccg
      481 atggcggatg ggacggcgaa acggatgatg caaagagagc acgacatgcg cgaacggccg
      541 gagaggattg tttcgggcgc ggcgtacgat gaggacgcgc gactggacgc cagtgtgcgg
      601 ccgaagacgc tcgacgaata cattggtcag aagcgtgtga aagaaaatat ttcgattgcg
      661 ctggaagcgg cacggaaccg cggcgaagcg ttggatcacg ttttgctcta tgggccgccg
      721 ggattgggaa aaaccacgct ggcgcaaatt attgcgaatg aattgaatgt gccgatcaag
      781 accagcgcag ggccgctgct ggaacgcaaa ggcgatttga cggcgatttt gacttcgctg
      841 gaaacgcgcg aagttttttt tctggatgag attcaccggc tgcaggcggc ggtggaagaa
      901 gtgctgtacc cggcgatgga ggattttcgc gtggacctgg tgatcggtca ggggccgggg
      961 gcgcgcatcc atccgtttca cctgaaccat ttcacgctgg tgggagcaac gactcgcgcg
     1021 gggctgatca cggcgccgct gcgttcgcga tttgggattg tgcaccggct ggacttttac
     1081 gagccggaag atctgctgat cattttgaag cggtcggcga aaatcttgca tatcgagatg
     1141 gaagaaggcg gagcggagga gattgcgcga cggtgccgcg ggacgccgcg cattgccaac
     1201 cggctgctac ggcgggcgcg agattttgcg gaagtgcgcg ccgacggaaa gattacgcgc
     1261 ggcgtggcgc aggaagcgct gtccatgctg ggcgtggacg gcaagggact agacgaagtg
     1321 gaccggaacc tgatgctggc gctgctggac aagtacggtg gcgggccggt gggggtgggg
     1381 acgctggcag ccgcgctctc cgaagaagag gatgcgatcg aggagatgta cgagccgtac
     1441 ctgatgcaga ttgggatgat tgaccggaca ccgcgagggc gtgtggcgac gccgcgggcg
     1501 tatgagtact ttgggcgaga gctgccgcgc cgacagcccc gattgtttta ggtcgctaga
     1561 ccccctcccc ctctttggag aaagcagtta cgggaggggc gggcgatagg aggggttggg
     1621 ctgcgatttt cacggagggt gttagcacgg aagttactga atagcaacag atattaggag
     1681 tacgactctg ggaaaacggg cagggcatga gtttgaggcg gaagcgggaa gaatggcgag
     1741 gtgggacagc aggcaagcca atggcaaagc gagcgccacg ctaattcgat ggaaatgaag
     1801 gggctttgcg aaacgactca ggaggtgggc cgggcatagt tgtagaccag aatgttggca
     1861 tgaaggtaag aaaccaggcg ggtgagaaaa gctgcctact ctcagataaa cttagttaaa
     1921 ttagatattt agagaaagtt tgcttaacca gtcgcctgag cgaagtcggc acgccgctga
     1981 tgccataagc gataaaggct gag
//
LOCUS       DASTPM010000259         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_902985, whole genome shotgun sequence.
ACCESSION   DASTPM010000259 DASTPM010000000
VERSION     DASTPM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902985"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..185)
                     /locus_tag="VFR42_04600"
     tRNA            complement(110..185)
                     /locus_tag="VFR42_04600"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Asn,seq:gtt)
     gene            584..1243
                     /locus_tag="VFR42_04605"
     CDS             584..1243
                     /locus_tag="VFR42_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU0368462.1"
                     /translation="MAQIFHRSTNFIARFSLFSGLFLAGLALTGVLGLARSPYFTRQN
                     ISRSQPVQFSHKHHVGDDGIDCRYCHTGVETSATAGVPATKTCMSCHSVLFNNVGYLE
                     PVRESYRSDTSIEWVKVHRLADYVYFNHSIHVNKGVGCSTCHGRVDQMPLIFQANTLL
                     MEWCLDCHKNTEKVLRPLDKVYDMTWTAPTNQAELGKKYHAERNIRSTTELTSCSTCH
                     R"
     gene            1252..>3236
                     /locus_tag="VFR42_04610"
     CDS             1252..>3236
                     /locus_tag="VFR42_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEU0368463.1"
                     /translation="MSDHEHRRDVDNHPPTAEERELRDFPLNLTAVRQKLGGQQGIQQ
                     GKKYWRTLDELAADPHFEDMLHREYPRGASEWDESVDRRDFLKLMGASLALAGMAGCG
                     LPPEQHIVPYVKQPDGLVLGKPQHYATVMPFSGDALGLLVESHEGRPTKIEGNPDHPA
                     SLGATDAFAQATILDLYDPDRAKTTTNLGQLSTWETFQGAAQGLATSQKAVNGAGLRI
                     LTGTITSPTVAGQIEALQKLYPQAKWHQWEPAVGDGAREGAKLAFGRVLNPVYRLDKA
                     EVIVSLDADFLASGPGRVRYMKDFYRQRHLTGPDGEMSRLYAVEPTPTVTGATADNRL
                     PLKANDVELFARALAAKIGPGGSATAPAGSEKFLNAAASDLLKHKGAGLVVAGAQQSA
                     EVHALAHLLNDLLGNAGATVYYTESVQANPMNHAESLRELCSDMARGKVDLLVILGGN
                     PVYDAPHDWDFAAKLKKVGTIVHLASHYNETSGYAHWHIPEAHFLETWGDARAFDGTY
                     SVIQPLIAPLYRAHSAFEVLAAFSDKPGVTAYDTVRERMKAMPGGADLEKFWRKSLND
                     GFVAGSAFAPVSISAKATLASLPAAKSTADTEFLFRPDPTIYDGRFANNGWLQELAKP
                     VTKITWDNAALMSPATAETLGVTHTIMSRGGEHGQVRS"
BASE COUNT          719 a          813 c         1053 g          651 t
ORIGIN      
        1 agttgatact tcgtttgggt tggaaacagg aattcggtct cggaacaaag gtggcgcaat
       61 ccgtattaaa actgcccgag cgcgcaatac caacattttt tggaaaattt ggctggggcg
      121 gaaggattcg aaccttcacc gtcctcatta acagtgaggt gtcctaccag ttggaccacg
      181 ccccagcagg gccgcgcgta aggggagagg cgcgctttgg taatgacttc gcgcaaaaac
      241 ggtcacgaag tatctacata gaataccacg ccgacgcagt tcgccaagag gcggcggtgc
      301 gacagatctg aaaatattca aataaccgca gaggaaggcg tgcacgaggc ggcatggtgt
      361 gttctaaatt cgaatgtttc aaagtgggtg aatcaaccga aaaaaaattg atgcacggaa
      421 aaaataattt gttttgcgcc gtggtgttgc aattcgaaaa gatttgttgg aaactatcgc
      481 gtcatattcg acccgggcac gcactcgtcc ggtgatgact gagggtggtt tctcctaggt
      541 gcccgctcct ttctcggaga ccccgccaga gtgcaacttg gacatggccc agatttttca
      601 ccgcagtacg aatttcatcg cccggtttag tctgttttca gggctattcc tggccggact
      661 ggcgttgacc ggagtgttag ggctggcccg ttcaccgtat ttcacgcggc aaaacatttc
      721 gcgttcgcag ccggtacaat tcagccacaa acatcacgtg ggcgatgacg gaatcgactg
      781 ccgctactgc cataccggcg tagagacgtc ggcaaccgca ggtgtccctg ccaccaagac
      841 ctgcatgagc tgccattcgg tgctattcaa taatgtcgga tatctggagc cggtgcgcga
      901 aagttatcgc tcggatactt cgattgaatg ggtaaaagtg caccggctgg cggattacgt
      961 gtatttcaat cacagcattc acgtgaacaa gggcgtgggc tgttcgacct gccacggccg
     1021 cgtggatcag atgccgctga tatttcaggc gaacacgctg ctgatggaat ggtgcctgga
     1081 ctgccacaag aatacggaaa aggttttgcg tccactggac aaggtgtatg acatgacgtg
     1141 gacggcgccg accaaccagg cggaacttgg caagaaatat cacgcggaac gcaatattcg
     1201 gagcacgacc gagctgacca gctgctcgac gtgccaccgt tagtggacgg tatgagcgat
     1261 cacgagcaca gacgcgacgt agataatcat ccgccaaccg cagaagaacg cgaattgcgc
     1321 gacttcccat tgaatttgac ggcggtgcgg caaaagctgg gcggacaaca aggcatccaa
     1381 cagggcaaaa aatattggcg aacgctggat gagctggcgg ccgatcctca ttttgaggac
     1441 atgctgcacc gggaatatcc gcgcggtgct tcggagtggg atgaatcggt agatcgccgt
     1501 gactttttga aattgatggg agcgtcgctg gcgctggcgg gaatggcggg atgcgggctg
     1561 ccgccggaac agcacatcgt tccttacgtg aagcagccag acgggttggt gctgggcaag
     1621 ccacagcact acgcgacggt gatgccgttt agcggcgacg cgctgggctt gctggtggaa
     1681 agccacgaag ggcgtcccac aaaaattgag ggaaatccgg atcatccggc gagcctgggg
     1741 gctacggacg cgtttgcgca ggcaacgatt ctggatttgt acgaccctga ccgcgccaag
     1801 acgactacga acctggggca gttgagcact tgggaaacgt tccagggcgc cgcgcagggc
     1861 ttggcaactt cacaaaaggc agtgaatggc gcgggactgc ggattttgac gggaacgatt
     1921 acgtcgccca ccgttgccgg tcagatcgaa gcgttgcaga agttgtatcc gcaagcgaag
     1981 tggcaccagt gggagcctgc agttggcgat ggcgcgcgcg aaggtgcgaa gctggcgttc
     2041 gggcgcgtgc tgaatccggt gtaccggctg gacaaggcgg aagtgatcgt ttcgctggac
     2101 gcggattttc tggcaagcgg gccgggacgc gtccgctaca tgaaagattt ttatcggcag
     2161 cggcatttga ccggtccaga cggcgagatg agccggcttt atgcggttga gccgactccg
     2221 acggtgaccg gcgcaaccgc ggataaccgg ttgccgctga aagcgaatga tgtggagttg
     2281 ttcgcgcggg cgctggcggc gaagattgga ccgggtggtt cggcaacggc accggcggga
     2341 tcggagaaat tcctgaacgc tgcggcttcc gatttgttga agcacaaggg cgcgggatta
     2401 gtggtagctg gggcgcagca atccgccgaa gtgcatgcgc tggcgcacct gctgaacgat
     2461 ctactgggga atgccggcgc gacggtgtac tacaccgagt cagtgcaagc gaatccaatg
     2521 aatcatgcgg aatcgctgcg ggaactttgt tcggacatgg cgcgcggcaa agtggatttg
     2581 ctggtgattc tgggcggcaa tccggtatat gacgcgccgc acgattggga ttttgcggcg
     2641 aagctgaaga aggtcggcac gatcgtgcat ctggcttcgc attacaacga gacgagcggc
     2701 tacgcgcatt ggcacattcc ggaggcgcat tttctggaga cttggggcga tgcgcgggcg
     2761 tttgacggca cgtatagcgt gattcagccg ctcatcgcgc cgttgtatcg cgcacattcg
     2821 gcgtttgaag tgctagcggc attttccgac aagccgggcg tgacggcgta cgacacggtg
     2881 cgcgaacgca tgaaggcaat gccgggcggc gcggacctgg aaaaattctg gcgcaagtcg
     2941 ttgaacgacg gatttgtggc cggctctgcg tttgcaccgg tgagcatttc ggcaaaggcg
     3001 acgctggcga gtttgccggc ggcgaagtcg actgcggata cggaattttt gttccggccg
     3061 gacccgacga tttacgacgg gcggtttgcg aacaacggct ggctgcagga attggccaag
     3121 ccggtgacca agattacctg ggacaatgcg gcgctgatga gtccggcgac ggccgaaacg
     3181 ctgggcgtga cgcatacgat tatgtcgcgt ggcggcgagc acggtcaggt gcgctc
//
LOCUS       DASTPM010000260         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_614585, whole genome shotgun sequence.
ACCESSION   DASTPM010000260 DASTPM010000000
VERSION     DASTPM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614585"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..625
                     /locus_tag="VFR42_04615"
     CDS             104..625
                     /locus_tag="VFR42_04615"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU0368464.1"
                     /translation="MNDLKKLIREVPDYPKPGILFYDLTTLLKDKQGFRTLIDRLCDH
                     YKGHTIDVVAGMEARGFIFAPALAYRLGAGFVPVRKPKKLPAKTASISYALEYGTDTL
                     EVHEDAIQKGTRVIICDDLLATGGTAAATAQLIQKLGGVVEGAAFAVELTFLNGRSKL
                     PGLDLFSLIQYDK"
     gene            complement(717..1454)
                     /gene="rlmB"
                     /locus_tag="VFR42_04620"
     CDS             complement(717..1454)
                     /gene="rlmB"
                     /locus_tag="VFR42_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU0368465.1"
                     /translation="MDKLTGIHAVKEALEAARPLEYVLVAKGRQDSRAEEIVHLARRN
                     GIPVRFEERAQLDRTAGTRDHQGVVALGAARAAGTLEDILASANQEKGKPGLIVLLDG
                     VEDPHNLGAIIRTALAAGAHGVVIPERRAAGLTDTVARASAGALAHLPVAKVTNLARS
                     MEELKEAGYWLVGLDERADKGYTEVDYTSPTGIVMGGEDGMHELTRKRCDFVVSLPTS
                     GPVKSLNVSVAAGVVLFEAVRQRHFRK"
     gene            complement(1550..>2810)
                     /locus_tag="VFR42_04625"
     CDS             complement(1550..>2810)
                     /locus_tag="VFR42_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0368466.1"
                     /translation="HNPDIHRTIDAVWRIESARIIASLTRITHDVGLSEELAQDAMVA
                     ALEQWPVSGIPDNPGAWLMAAAKNRAIDRFRRNKLLERKHEELGRELKSQQDAAMADF
                     EIADDDISDDLLRLVFISCHPLLSTESRAALTLRLLGGLTTQEIARAFLTPEPTIAQR
                     IVRAKRTLTEARVPFEVPRGLDRAARLSSVLEVIYLIFNEGYSATAGDDWMRPALCED
                     ALRVGRILAELAPQEPEVHGLVALMEIQASRSKARVDAAGEPVLLLEQDRSQWDQLLI
                     HRGFDAIERVEKLGGANGPYALQAAIAACHARARSGEETDWPRIVRLYEELERLNPSP
                     IVELNRAVAVAMASGPAAGLELVDKLVSEPSLKAYHLLPSVRGDFLFKLGRLEEARRE
                     FERAAELTRNARERKLLLDRAQACARQ"
BASE COUNT          548 a          973 c          707 g          582 t
ORIGIN      
        1 gtcgccgagc atccagccga aattgatccg caattttcta gcgagttcag tatcacctac
       61 gattcttgaa ccaaatacct gcaacagcga gggagccgtc cgcatgaatg atttaaaaaa
      121 gctgattcgc gaagtacccg attatccgaa gccgggaatt ttgttttacg acctgactac
      181 tctgttgaaa gacaagcagg gtttccgcac tttgattgat cgcctgtgcg atcactataa
      241 aggccacact atcgacgtcg tcgcgggcat ggaagcgcgc ggctttatct tcgcaccggc
      301 gttggcctac cgtctcggcg ccggattcgt cccggtgcgc aagcccaaga agttgcctgc
      361 gaaaaccgcc tcgatttcct acgcgctgga atatggcact gacacgctgg aagttcacga
      421 agacgccatt cagaaaggca ctcgcgtcat catctgcgac gacttgctcg ccaccggtgg
      481 cactgccgcc gctaccgcgc aactcatcca aaaacttggc ggcgttgttg aaggtgccgc
      541 cttcgctgtc gaactcacgt ttctcaatgg ccgctccaaa ctccccggcc tagacttatt
      601 ttctctgatc caatacgata aatgattgcg ccgtggcgca aacgtctcta tgaactctgc
      661 gcctcctcct cttcctctgt gttaagtttt tttctgattc ttctgaagac ctgaatttat
      721 tttctaaagt gccgttgccg caccgcctca aacagcacca cgcccgcagc caccgacaca
      781 ttcaacgatt tcaccggccc gctcgtcggc aacgacacca caaaatcgca gcgcttccgc
      841 gtcaattcgt gcattccatc ttcgccgccc atcacaattc cggtcggcga cgtgtaatcc
      901 acctcggtgt atcccttgtc cgcacgctcg tccaatccca ccagccaata ccctgcctct
      961 ttcaactcct ccatcgatcg cgccaggtta gtcactttcg caaccggcaa atgcgccagc
     1021 gctcccgccg acgcccgcgc caccgtgtcc gtcagtcccg ctgcacgtcg ttcgggaatc
     1081 accactccgt gcgctcccgc cgccagcgcg gtgcgtatga tcgcgcccag attgtgcggg
     1141 tcctccacac catccagcag gacaatcaat cccggcttac ctttttcctg gttggcgctg
     1201 gccagaatgt cttcgagcgt gcccgcagct cgcgccgcac ccagtgccac cactccctgg
     1261 tggtctcgcg tcccagcagt gcgatccaac tgcgcccgct cctcgaagcg caccggaatt
     1321 ccgttgcgcc gcgccaagtg cacgatttct tccgcgcggc tgtcttgccg gcccttcgcc
     1381 accaatacgt attccagcgg ccgcgctgcc tccagcgcct ctttcaccgc atgtatccca
     1441 gtcagcttgt ccatggattg acagtgtagc ttcgccgctc ctcgccgcaa aaccgcggcg
     1501 agccccgaag gtaaccatca gtcgtagcgg ccaccttcag gtggcctttt cattgcctgg
     1561 cacacgcctg cgcacggtcc aatagcaatt tgcgttcgcg cgcattgcgc gtaagctcgg
     1621 ccgcacgctc aaactcgcgg cgcgcctctt ccaaccttcc caacttaaaa agaaaatcgc
     1681 cgcgcacgct aggcaacagg tgatacgcct tcagagaagg ctcggacaca agtttatcta
     1741 ccagctcaag ccctgccgct ggtccagacg ccattgctac ggcgaccgcg cgattcagtt
     1801 ccacgatcgg cgaaggattc agccgctcca gctcttcata cagccgcaca atgcgcggcc
     1861 aatccgtctc ttcgccgctg cgcgcgcggg catggcacgc cgcaatcgcc gcctgcagcg
     1921 catacggccc gttggccccg cctaatttct ccacacgctc aatcgcgtcg aacccgcgat
     1981 gaatcagcag ctgatcccac tgcgaacgat cctgctccaa cagcagcacc ggctcgcccg
     2041 ccgcgtccac gcgagccttg ctgcgcgacg cctgaatctc catcaacgct accagcccat
     2101 gcacctcagg ctcctgcgga gccagttccg ccagaatccg tccgacccgc agcgcgtctt
     2161 cgcaaagcgc gggacgcatc caatcgtcgc cggcagtcgc cgagtagcct tcattgaaaa
     2221 tcaggtagat gacttccagc accgatgaca gccgcgccgc gcgatccagc ccgcgcggaa
     2281 cttcaaacgg aacgcgtgcc tccgtcaaag ttcgtttcgc tcgtacgatg cgctgcgcga
     2341 tggtcggctc cggagtcaga aatgctcgag caatttcctg cgtggtcagc ccgcccagca
     2401 atcgcagcgt caacgcggcg cgcgactcgg tggacagaag tggatgacag gaaatgaaaa
     2461 ccaggcgcag cagatcgtcg ctaatgtcat cgtccgcaat ctcaaaatcc gccatggcgg
     2521 catcctgctg ggatttcagc tcgcggccca gctcttcatg tttgcgttcc aatagtttgt
     2581 ttcggcgaaa ccggtcgatt gcgcggttct tggcggcggc catcagccac gcgccgggat
     2641 tgtcggggat gccggacact ggccattgct cgagcgccgc caccatcgcg tcttgtgcca
     2701 gctcttcaga caaaccgacg tcgtgggtga tgcgagtcag cgaggcgatg atcctggctg
     2761 actcgatcct ccacaccgcg tcgatcgtcc ggtgaatgtc cgggttgtgc
//
LOCUS       DASTPM010000261         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_931562, whole genome shotgun sequence.
ACCESSION   DASTPM010000261 DASTPM010000000
VERSION     DASTPM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931562"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..196)
                     /locus_tag="VFR42_04630"
     CDS             complement(8..196)
                     /locus_tag="VFR42_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368467.1"
                     /translation="MELTLAMPRQRTTAPILVVSILLGLLLSPLLERALENWMPANVA
                     STQQQGDGSSWDPRTMWY"
     gene            complement(253..747)
                     /locus_tag="VFR42_04635"
     CDS             complement(253..747)
                     /locus_tag="VFR42_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU0368468.1"
                     /translation="MAATPTHRASTGERINTSETTGHLVKTLGERGIRLTRQRRVILQ
                     VMDNAEQHLDVDQIFERAKRIDSGVHLVTVYRTIELLKKQGLIDELDLLHLRGDRHYY
                     ETHGPRDHIHVACLKCGKVREFESRLYEQLKSQIEKDFSMKVTISRTEVGGYCAECLA
                     SGAS"
     gene            complement(806..2128)
                     /gene="rseP"
                     /locus_tag="VFR42_04640"
     CDS             complement(806..2128)
                     /gene="rseP"
                     /locus_tag="VFR42_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU0368469.1"
                     /translation="MALDILIGVALVLGVIILIHEWGHFIMARLFGVRVDVFSIGFGP
                     RLFGWKKGATDYRISALPLGGYVRMAGQDPSEVDSGNTAPTGASDELMSKPRWQRALI
                     AFGGPGVNFIFPILLLAAFYALVGIPHSMVLRQPLEVSAFSATLKQPSPLEVGDKVIE
                     MNDLRNPTWESALESLDHVPLGSTITLKVEAKDGQQRTVQTALKDAGSFDQIFGFIPF
                     GPVIDDVSRRSPAKRAGLKADDRVVEANGQPVTYWGQFVDAIHQSKGQPVALKVLRKG
                     QTIPITVTPQQGLTDTKDATWQIGVANQNDLVYDRTSLGLATTYAFRKTGDFFVQTLG
                     VVGKLFSGRASVKQLQSVVGIAQISGDAVRKGPPQVVFLMAFISVNLGILNLLPIPIL
                     DGGMIMMLTIEGLLRRDLSLTVKERFVQVGFVFLLLLFAYVMYNDVVG"
     gene            complement(2157..>3154)
                     /gene="dxr"
                     /locus_tag="VFR42_04645"
     CDS             complement(2157..>3154)
                     /gene="dxr"
                     /locus_tag="VFR42_04645"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU0368470.1"
                     /translation="AALLGEKGIAPLPAIHHGREGMLAAGTHGAADIVVSAAVGVVGL
                     EATYEAVKLGKTIALSNKEVLVAAGEIVMAAAHNAGKELLPVDSEHNAVHQCLRGGKH
                     SEVRKLVLTASGGPFRKTPIHKMAEVTVEQALAHPNWKMGNRITVDSATMMNKGFEVI
                     EARWLFDMKPAQVDVVIHPQSTVHSMVEFVDGSVLAQLGATDMRMPIQYALTYPERVA
                     SNQVDLDWVKLKHLDFEQVSTRRYLCLRLAREAMKKGGAWPCALNAADEVAVAAFLER
                     RIPFLGIAEVVEKVLERTPHVKIEKMDDVLAADAEARRIGREEVNRLASRAVAVS"
BASE COUNT          666 a         1054 c          778 g          656 t
ORIGIN      
        1 ttgcggctca ataccacatc gttcgcggat cccaactcga gccatcacct tgctgttgag
       61 tactcgccac gttcgccggc atccagtttt ccagcgcacg ttccagcagc ggcgaaagca
      121 gcaatcctaa aagaatcgaa acaaccagga tgggagccgt cgtccgctgc cgcggcattg
      181 ccagcgtcag ttccatagcc gcttagacac cgcatccaac aataagtttc cgtgcaaacc
      241 ttacaggagg gcttacgacg cgccgctggc cagacattcg gcgcagtatc cgccaacttc
      301 ggtacgggaa atagtgacct tcatgctgaa atctttttcg atctgcgatt tcagctgctc
      361 gtaaagccgg ctctcaaact cgcgcacctt gccgcacttc aggcatgcca catgaatgtg
      421 gtcgcgcggt ccgtgtgttt cgtagtagtg gcggtcgccg cgcagatgca gcaggtccag
      481 ctcgtcaatc agtccctgct ttttcaaaag ttcaatcgtc cggtacaccg tcaccaggtg
      541 tacgccggag tcgatccgct tggcgcgttc gaagatctgg tcgacatcca ggtgctgctc
      601 ggcattgtcc atcacttgca ggatcacgcg acgctgccgg gtcaagcgga tgccccgctc
      661 ccccagcgtt ttcaccaaat gtcccgtagt ttccgaagtg ttgatgcgct cgccggtaga
      721 ggcgcgatgc gtgggcgtgg ctgccatgaa attcctcaac ttgctgcagc aaaacgcggc
      781 ctaaccgtca aatccgcggt tcgtttcatc caaccacgtc attgtacatg acgtaagcga
      841 agagcaacaa aaggaacaca aatcccacct gaacgaagcg ctctttcacc gtaaggctca
      901 ggtcgcggcg tagcaatccc tcaatcgtca gcatcatgat cataccgccg tccagaatcg
      961 gaatcggcag caaattcagg atgcccaggt tcacgctgat aaacgccatc aggaaaacca
     1021 cttgcggcgg acccttgcgt accgcatcgc cggaaatctg cgcgatgccc accacgctct
     1081 gcagctgctt cacggaagcg cggccgctga acaatttgcc gacgacgccc agcgtttgca
     1141 cgaagaagtc gcccgtcttg cgaaacgcat aggtcgtcgc caagccgagc gaagtgcggt
     1201 cgtacaccag atcattttga ttggccacac caatttgcca ggtggcgtcc ttggtgtcgg
     1261 tcagcccctg ctgcggcgtc accgtgatcg gaatcgtctg tcctttgcgc aaaaccttga
     1321 gcgcaaccgg ctggcccttg gactggtgga tcgcgtccac aaattggccc caataagtca
     1381 ccggttgccc gttcgcttcg accacgcgat cgtcggcctt caaccccgca cgctttgccg
     1441 gcgacctccg gctgacatcg tcaatcaccg gtccaaacgg gataaatcca aaaatctggt
     1501 caaacgagcc cgcatctttc agcgcggttt gcacggtacg ctgctgtccg tctttcgcct
     1561 ctaccttgag cgtaatcgtc gaaccgagcg gcacatggtc cagactctcc agtgcgcttt
     1621 cccatgttgg attcctgaga tcgttcatct caataacttt gtcgccgact tcgagcgggc
     1681 tcggctgctt cagcgtcgcc gaaaatgcgc tcacttctag cggctgccgc aagaccatcg
     1741 agtgcgggat gccaaccaag gcgtaaaatg ccgcgagcag caggatggga aaaataaaat
     1801 tcactcccgg cccgccaaac gcgatcaacg ccctctgcca gcgcggtttg ctcatcagct
     1861 catcggaagc gcccgtcgga gccgtattgc ccgagtccac ttccgaggga tcctgccccg
     1921 ccatccgcac atacccgcca agcggtagcg cgctgattcg atagtccgta gcccccttct
     1981 tccagccgaa cagccgcggc ccgaagccaa tcgagaaaac gtccacccgg acgccaaaca
     2041 ggcgcgccat gatgaagtgc ccccattcgt gaatgaggat aatcacgccc agcaccaggg
     2101 cgacgccaat caaaatatcc aacgccattt cgggcaacct ctcgacctga gttaactcac
     2161 gaaaccgcca cggccctact ggccaggcga ttcacttcct ctctccctat tcgacgcgct
     2221 tccgcatccg cggcgagcac atcgtccatc ttctcaatct tcacgtgcgg tgtgcgctcc
     2281 agcactttct ccacaacttc cgcaataccc aaaaacggaa tccgccgttc caggaacgcg
     2341 gcaaccgcaa cttcatcggc ggcgttcaat gcgcagggcc aggctcctcc ctttttcatc
     2401 gcttcgcggg ctaggcgcag gcagagatac cgccgcgtag agacctgctc aaaatccaga
     2461 tgtttcagtt tgacccagtc caggtccacc tggtttgatg ccaccctctc tggataggtt
     2521 aacgcatatt ggatgggcat gcgcatgtcg gttgccccga gttgtgccag aaccgaaccg
     2581 tccacaaact ccaccatcga gtgcaccgtc gactgcgggt ggataaccac atccacctgc
     2641 gccggcttca tatcaaacag ccagcgcgct tcaatcactt caaatccctt gttcatcatg
     2701 gtcgccgaat ccaccgtaat ccggtttccc attttccagt ttggatgcgc caacgcttgc
     2761 tccaccgtca cttctgccat tttgtgaatg ggagtcttgc gaaacgggcc gccggatgcc
     2821 gtcagcacca gcttgcgaac ctcagagtgc ttcccgcccc gcaaacactg atgcactgcg
     2881 ttgtgctcgc tgtctactgg cagcaattcc ttgcctgcat tgtgcgccgc ggccattacg
     2941 atttcgcccg cggccaccag cacttcctta ttcgacaacg cgatcgtctt gcccagcttc
     3001 accgcttcgt acgtcgcttc cagcccaacg acgcccaccg ccgcggagac cacaatgtcc
     3061 gccgccccgt gcgtccctgc cgccaacatc ccttcacgtc catgatggat tgccggcaac
     3121 ggcgcaattc ccttttcacc taaaagcgca gcca
//
LOCUS       DASTPM010000262         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1162661, whole genome shotgun sequence.
ACCESSION   DASTPM010000262 DASTPM010000000
VERSION     DASTPM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162661"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFR42_04650"
     CDS             complement(<1..925)
                     /locus_tag="VFR42_04650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0368471.1"
                     /translation="MDSARWQRIQTLFHEAADLPPAQQHAFLQENCGDDQALFAEVVQ
                     LLREDAEASSLLERDVAHVAHEVLGDGSTPAGEQFGRYRIKRTLGAGGMGIVYLAERE
                     HLGSLVAIKILRDAWLSPARRERFTFEQRTLAQLNHPYIARLYDADTSPDGTPFFVME
                     YVEGVPLTAYCTQHRCSIDDRLKLFREVSEAVVYAHQHALIHRDLKPSNILVKEDGTV
                     RLLDFGIAKQLEVLGEAVDQTMTGLRLMTPAYASPEQIRGQQVGIQTDVYSLGVVLYE
                     LLSGRLPFDLSSKTPAQAEKVLTEQEPQRPSL"
     gene            complement(969..1556)
                     /locus_tag="VFR42_04655"
     CDS             complement(969..1556)
                     /locus_tag="VFR42_04655"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0368472.1"
                     /translation="MAMENSGWNGSFEGLHSHDVTVPPGDRRALDHLFSVTYEELRRL
                     ASSVRRGDPSATLSPTALVNEAWLKLADSPQVASTSRLHFKRIAARAMRQLLVEAARR
                     RNADKRNGGAEVTVTFDESVQANPSGSRELLALNSALDELARMNPRQAVMVESRFFGG
                     LDIPETASLLKISEATVQRDWRAARAWLAHELRQA"
     gene            1801..>2893
                     /locus_tag="VFR42_04660"
     CDS             1801..>2893
                     /locus_tag="VFR42_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368473.1"
                     /translation="MRTSQRVSLLSSIVFLCVTAGSAQAQQQPGVSPSVWAAPAQPQA
                     AQASSAFQSACGNQPMCYDAPDFAVAVVDFRMSMASGYKLMDATLRFVNKTNQPLILG
                     YLDGSAVALDDQGNRYAPYGNRGLAGIGLVNGNSADPKFVLQPGGAGDARWELLWRPG
                     PQDPIGSAFDLSMTIREVNTLVGGQHALGGEIPLRFQGLANGVVASGGAPAGGTAGVA
                     GVAGASPMLAGGSSGLPACGPSGNAAGTMTAIAGAANSVGGQQTANATNTATNTAGNA
                     MSQLAGLKSIFGKKNATAPATSASGANCIPTATANAMPAAVGTSTAPAAAGQAVTTAP
                     TAVAPVAAAPVGVAPAVAVPATAGKPTANA"
BASE COUNT          550 a          933 c          869 g          541 t
ORIGIN      
        1 caagagacgg ccgctgcggc tcttgttccg tcagcacttt ttcggcttgc gccggcgttt
       61 tagaggacaa atcaaacggc agccggccac ttaacaactc gtagagcacc actcccaatg
      121 aatagacatc cgtctggatc cccacctgtt ggccgcgaat ctgctccggc gacgcataag
      181 ccggagtcat caagcgcaag ccggtcatgg tctggtcgac cgcttcgccc aggacttcca
      241 gttgcttggc gataccaaag tccagcagcc ggacggtgcc atcttctttc accagaatgt
      301 tcgaaggctt caggtcccgg tgaatcaggg cgtgctggtg cgcgtagacc actgcttctg
      361 aaacttcgcg gaaaagtttc agccgatcgt cgatgctgca gcgatgttgc gtgcagtatg
      421 cggtcagcgg gacgccttcg acatactcca tcacgaaaaa gggcgtgcca tcgggcgaag
      481 tgtcggcgtc gtacaggcgc gcaatgtaag gatgattgag ctgtgctagc gtgcgctgtt
      541 cgaacgtaaa ccgttcgcgg cgcgcgggcg ataaccacgc atcgcgcaga atctttatcg
      601 ccaccaggct gcccaggtgc tcgcgttcgg ccaggtacac aatccccatt ccgcccgcgc
      661 ccagggttcg tttgatccgg tagcgtccaa attgctcccc agcgggcgtc gagccatcgc
      721 ccagcacttc gtgcgcaaca tgcgcgacat cccgctccag cagggagctc gcctccgcat
      781 cttcgcgaag cagttggacg acttccgcga agagcgcctg gtcgtcgccg caattttctt
      841 gcaggaatgc gtgctgttgt gcgggcggca ggtcggccgc ctcatgaaac agagtctgga
      901 ttctctgcca gcgagcgctg tccatcctca caccacttcc gcaaccggca actttccact
      961 aaagtcagct acgcttggcg aagttcgtgt gccagccaag cccttgccgc ccgccaatcg
     1021 cgttgcacag tagcctcgga aatcttgagc agcgaagcgg tctccgggat gtccagcccg
     1081 ccgaaaaagc ggctttccac cataaccgcc tgacgcggat tcattcgcgc cagctcatcc
     1141 agggcgctgt tcagcgccag cagttcgcgg ctgccggagg ggttggcttg taccgactcg
     1201 tcaaacgtta ccgtcacttc tgcgccgccg ttgcgcttgt cggcgttgcg gcgccgagct
     1261 gcctcgacca aaagttgacg catggcgcgc gcggcaatcc gcttgaaatg cagtcgggag
     1321 gttgacgcaa cctgcggaga gtccgccagt ttcagccagg cctcattcac cagggccgta
     1381 gggcttagtg tggcgctcgg atcgcctcgg cgcaccgagg acgccaaacg gcgaagctct
     1441 tcataggtga cgctgaaaag gtggtctaac gcccgtcgat ccccgggcgg tacggttacg
     1501 tcgtggctgt gtaagccctc gaaactgcca ttccagccgg aattttccat cgccatgacg
     1561 gtatttctcc gccgtttgcg caatgcgaag taggaagcaa aacactcagg accgacagtg
     1621 cggagcctgg gactgccgct cgcgcagtgg ccgaactcta tcacccgaag cggtttcccg
     1681 gcaacgggca gattgcggct tcaccgcggg catagtcacc tccgccagaa ggtttgagtg
     1741 cgctgtctgg gccctccgag gacgcggtgg gcccacaaag tctttcgaag gagcattgcg
     1801 atgagaacca gccaacgagt gtcacttctt tcctcgatcg ttttcctttg tgtcacagcg
     1861 ggaagtgccc aagcgcagca acagccaggg gtttcgccat ctgtgtgggc ggcaccggct
     1921 cagccgcaag cggcgcaggc tagctccgcg tttcaatcgg cctgcggaaa ccagccgatg
     1981 tgctacgacg cgcccgattt tgccgtcgct gtagtcgact tccgcatgag catggctagc
     2041 ggctataagt tgatggacgc cacgctgcgc ttcgtaaaca aaaccaatca gccgctgatt
     2101 ctcggctacc ttgacggttc cgccgtggca ctggacgacc aaggcaatcg ctacgcgcct
     2161 tatggcaatc gcggtctcgc aggcatcggg ctggtcaacg gcaactctgc cgatccgaag
     2221 tttgtcctac aacccggcgg cgccggcgat gcacgctggg aactcctgtg gaggcccggc
     2281 ccgcaggatc cgatcggcag cgcctttgac ctctcgatga ccatccggga ggtaaatacg
     2341 ttggtgggtg gccaacacgc acttggcggc gaaattcctc tgcgattcca gggcctagcc
     2401 aacggcgtgg tcgcttctgg cggagccccc gcgggaggaa ccgcgggcgt cgccggcgtg
     2461 gcaggagcat cacccatgct ggctggcgga tccagcggac tccccgcgtg tgggccttct
     2521 ggaaatgccg ctggcaccat gacggcaatc gcgggagcgg ccaactcggt gggcggtcag
     2581 cagaccgcga atgcgaccaa caccgcaacg aacaccgccg gcaatgccat gtcccaactc
     2641 gccgggctca agtcgatttt cggcaagaag aacgccacgg ctcccgcaac cagtgcgtcc
     2701 ggcgcaaatt gcatccccac ggcgactgcc aacgcaatgc cggccgccgt cgggacgtcc
     2761 actgcgccgg ccgcagcggg ccaagccgtt acaaccgccc cgactgcggt agcaccagtt
     2821 gcggcggcac cggtgggtgt agcgccagcg gttgctgttc ctgccactgc tggaaagccg
     2881 accgccaacg cca
//
LOCUS       DASTPM010000263         2999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_787801, whole genome shotgun sequence.
ACCESSION   DASTPM010000263 DASTPM010000000
VERSION     DASTPM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787801"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..2075)
                     /locus_tag="VFR42_04665"
     CDS             complement(354..2075)
                     /locus_tag="VFR42_04665"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU0368474.1"
                     /translation="MAPKYIFVTGGVVSSLGKGVAASSIGCLLESRGFKVTLQKCDPY
                     LNVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFTHAKLTRDNNWTTGRIYETILTK
                     ERRGDYLGKTVQVIPHVTDEIKNIIKKVSADVDVVIVEIGGTVGDIESLPFLEAIRQM
                     RLELGAENTLFVHVTLVPYIAAAGEQKTKPTQHSVKEMLGIGIQPDILLCRSDRHIPS
                     ELKKKVALFCNVAESCVISMEDVDTIYAVPAELGREGLDKQILRLLKLEERPQNFQPW
                     LDLIQRIHHPAGEARIAIVGKYVQLEDAYKSLREALLHGGLAHNHKTVIDWIESEEID
                     SAETAASRLRAYDGVLVPGGFGKRGIEGMIHTIRYARENKIPFLGICLGMQCATIEYA
                     RDVAGLKQANSTEFDQQTPFRVIYKLRELLGIDEMGGTMRLGAWQCKLEAGSFAAKAY
                     GRLEISERHRHRYEFNREHEKTLVAAGLKITGRTPDENYVEIVEAPEHPWFLGCQFHP
                     EFKSKPLEPHPLFAAFIGAALEHKKARMKPASSGMGAAAGGEGMHAPAVETPRTPVVR
                     AAPGVQA"
     gene            2570..>2999
                     /locus_tag="VFR42_04670"
     CDS             2570..>2999
                     /locus_tag="VFR42_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368475.1"
                     /translation="MNLSPRIRRYAACLCLLAAPAAAVAQRPHANATTAVSGGYNLAN
                     ESSLAGTILSYTENSRTPPLGTHVLLQTASGNVDVHLGDARVLHQAKLNLAQGMSVRF
                     VGQSQAVGKNNVFLARLVQVGSQVVAVRSTHGLPVSPGVAR"
BASE COUNT          564 a          931 c          810 g          694 t
ORIGIN      
        1 ccatcgcgtc acggtttctc cgcctgcaaa cacagaaact tcgcctaccg gccatccgat
       61 agcttgaaat gctacctgaa tttcagcaac gtcgatcaaa ccatcgcctt ctacgcacca
      121 gaccaaggtg cgcttgccgc cgaattcgcc gacatggagc agcattcagc atgccgcttc
      181 gggatacgca cgacggctta cgcggcttcg agatttgcgg tccccacggc tacgtcttgt
      241 tggttcgggg cggtcaaggt agtcgggccc atcaccagag ttggccgggc gcttccgcgc
      301 agcgcctggt gcgcgaagct cggaccgcct gaccctatgc ctgttaacta gctctacgct
      361 tgcacgccag gcgcagcgcg tacaactggc gttcgcggcg tttccacagc cggcgcgtgc
      421 atcccttcgc cgccagccgc tgctcccatg ccggaacttg ccggtttcat ccgagccttc
      481 ttatgttcga gcgccgctcc aatgaacgct gcaaacaggg ggtgcggctc gagcggcttg
      541 gacttgaatt ccgggtggaa ctggcagccc aggaaccatg gatgttccgg ggcttccacg
      601 atttccacgt aattttcgtc cggagtgcgg ccggtgattt tcaatcctgc cgcaaccagc
      661 gtcttttcgt gctcgcggtt gaattcatac cggtggcggt ggcgctcgct gatctccagc
      721 ctgccgtacg ctttcgccgc aaacgaaccg gcctccagct tgcactgcca cgcacccagc
      781 cgcatggtgc cgcccatttc gtcgatgccc agcagttcgc gcaatttgta aatcacgcgg
      841 aaaggagtct gttggtcgaa ttcggtgctg ttcgcctgct tcagtcccgc aacgtcgcgc
      901 gcatattcga tcgtcgcgca ctgcatgccc aggcaaattc caaggaaggg aatcttgttt
      961 tcccgcgcgt agcgaatcgt atgaatcatt ccttcaatgc cgcgcttgcc gaatccgccc
     1021 ggcaccagga cgccgtcgta ggcgcgcagc cggctcgccg cggtctcggc cgaatcgatc
     1081 tcctccgatt cgatccaatc aattacggtc ttgtgattgt gcgccagccc gccgtgcagc
     1141 agcgcttctc gcaagctctt gtacgcatct tccagctgca cgtacttccc gacgatcgca
     1201 atgcgcgctt cgccggccgg atgatgaatg cgctggatca aatccaacca cggctgaaaa
     1261 ttctgcggac gctcttccag cttcaacaag cgcaagatct gtttgtccag tccttcgcgg
     1321 cccagttccg ccggcaccgc gtaaatcgtg tccacatctt ccatagagat gacgcacgat
     1381 tcggccacgt tgcagaacaa cgccactttc tttttcagtt cggagggaat gtgccggtcg
     1441 ctgcggcaca gcagtatgtc cggctgaata ccgatgccca gcatctcttt cacactgtgc
     1501 tgcgtcggct tggtcttctg ttcgcccgcc gccgcaatgt aaggaaccag cgtcacatgc
     1561 acaaacaaag tattttccgc gccaagttcc agccgcatct ggcggatagc ttccagaaac
     1621 ggcagcgact caatgtcgcc caccgttccg ccgatttcga caatcaccac gtccacgtcg
     1681 gcggaaacct tcttgatgat gtttttgatt tcgtcggtaa cgtgcggaat cacctgcacg
     1741 gttttgccca ggtaatcgcc gcgccgctct ttggtcagga tcgtttcgta gattcttccg
     1801 gtggtccagt tgttgtcacg cgtcagcttg gcgtgcgtga agcgctcgta atgtccgagg
     1861 tccaggtccg tctccgcgcc gtcttccgtc acgaacactt cgccgtgctg gaacgggctc
     1921 atcgtgccgg gatcgacgtt cagatacgga tcgcactttt gcagggtgac tttgaaacca
     1981 cgactttcga ggagacaacc gatggaggag gctgctacgc ctttacctag tgaggaaaca
     2041 acaccgccag taacaaatat atattttggc gccatccgtg tagtccgccc gggttccagg
     2101 gattgcttta caaaccctgg gataacgaaa accccgcttt ggactcttct tacctctcag
     2161 gcctgccatc aacagcaagc cataggtttc aggtttgttc aacaaacctg agtacgatga
     2221 cgacgtacgc acaattatgg gcacgcggcg atattctgtc aattccaact cttggcgtct
     2281 ataaatgtaa atttcttatc cacgccgaaa ccttttccgc gcaaaggggt tccaactttg
     2341 cgagggtctt tcccagtccg gacgctgact cttccggcac acccaccgtt ggaacggaaa
     2401 cggcggcagc aaccctgcca tctaaactgg gaaggtaccg caaagttcga tctttcgctt
     2461 ggttgtgcca cgcccgcttt ctggcgccgg gctggcttcg gggtcttgaa cctcgacatt
     2521 gttttgacta cagcgcccag ccattgggcc tcgattgggg tgaattgaaa tgaatctgtc
     2581 gccgcgcatc cgtcgttatg ccgcatgttt gtgtctactg gccgctcccg ctgcggcggt
     2641 ggcacagcgt ccgcacgcca acgccactac ggcagtttct ggcggctaca atctggcgaa
     2701 cgagtcttcg ctagcgggca cgattttgag ttacacggaa aattccagga cgccgccgct
     2761 cggcacgcac gttcttttgc aaaccgcttc cggaaatgtg gacgtgcatc tgggcgatgc
     2821 tcgggtgctg catcaagcca aactgaatct tgcgcagggc atgagcgttc gcttcgttgg
     2881 ccagtcgcag gccgttggca agaataatgt tttcctggcg cgcctggtgc aagtcggctc
     2941 gcaggtagtc gcggttcgct cgacgcacgg tttgccggtt tcgcccggcg ttgcgcgtg
//
LOCUS       DASTPM010000264         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_845427, whole genome shotgun sequence.
ACCESSION   DASTPM010000264 DASTPM010000000
VERSION     DASTPM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845427"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFR42_04675"
     CDS             complement(<1..570)
                     /locus_tag="VFR42_04675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0368476.1"
                     /translation="MGPYEIVAPLGAGGMGEVYRARDTRLDRDVALKVLPRHLSSDAS
                     LKQRLEREAKAVSKLSHPHICTLHDIGHQDGTDFLVMEYLDGETLEHRLLRGPLPVQQ
                     ALRYAAQIADALAKAHKLGITHRDLKPANVMLTKSGAKLMDFGLAKQTGPAPLAAALT
                     EMTMEQSKLTGEGTIVGTFQYMAPEQLEGK"
     gene            complement(1033..2136)
                     /locus_tag="VFR42_04680"
     CDS             complement(1033..2136)
                     /locus_tag="VFR42_04680"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0368477.1"
                     /translation="MKKVSTAAARQSRNICQQKLTIGLDLGDRNSWYCVVDEAGQIQL
                     EQRVRTSAKALQEAFGAMPHCRIALETGTHSPWISRLLAELGHEVIVANARKVRLIGE
                     SRKKDDRLDAQTLARLARIDPELLCPVKHRSAQAQADLAVIRARAGLVRARTGLVNTV
                     RGLAKSYGERLRGCNVRNMNPEKAEGLSPELQRALEPLLSAIEELSQRIGEYNERIET
                     LAQQSYPKVALLKQIKGVGTLIALTFLLTLEDPHRFRRSRDVGCYLGLQPGRRNSGQS
                     EPQLHISKEGDPYLRTLLVQGAQHILGPFGVDCDLRRWGLKLAERGGRNGKKRAIVAT
                     ARKLAVLLHHLWVSGEVYEALHNTSRTTAAAAA"
BASE COUNT          400 a          740 c          634 g          547 t
ORIGIN      
        1 tttcccttcc agttgctcag gcgccatgta ctggaacgtc ccgacgatcg taccctcgcc
       61 ggtcagtttg gactgctcca tggtcatctc agtcagggcc gcggccagcg gcgccggccc
      121 agtctgcttg gcgagaccga agtccatcag cttggcgccg ctcttggtca gcatgacgtt
      181 ggcgggcttc aaatcacggt gggtaatccc cagcttgtgc gccttggcta gtgcgtcggc
      241 aatctgtgcg gcatagcgca acgcctgttg gacgggcaac ggtccgcgga gcaggcggtg
      301 ttcgagggtc tcgccgtcca ggtactccat gacgagaaaa tcggtgccgt cctggtggcc
      361 gatatcgtgg agggtgcata tgtggggatg cgagagtttg gagaccgctt tcgcttcgcg
      421 ttccagccgc tgcttcaaag atgcgtcgga ggaaagatgc cgcggcaaga ctttaagggc
      481 aacatcgcgg tcgagtcgag tatcgcgggc gcgatacact tcgcccatgc cacccgcgcc
      541 tagcggagcg acgatttcgt aaggccccaa ctttgtgcca gacgagaggg ccatcggtgg
      601 acgacattat agtctcaaag aagaccaagc accgaattgc ggatggatcg tcggcattcg
      661 gtacgacatt gtagttggcg taataatcgc cattaaccaa ttgaccgtgg gtcaatgagt
      721 ataaacgcct tccatgagaa ggccggtcgg agtcaagtcg attcttcgtt cgtcttgtga
      781 cacaaaggtt tttctcccgc cgagtagcca ctgtcgccat gcttccatcc gcactctcga
      841 ggagcgaatc gtttcgctcg gtgcatgttg ggcgtccacg tttcagccgg tgctgctatc
      901 tgatttgcga cctgtcgttg cacttcaaag tgcaacgtgg gccaggcgat taacgcagtc
      961 accggaactc ggctttggct tttgcctttt ctcttcctgg aacggttttg gggttgttct
     1021 tgcctttttg ttttacgcgg ctgcggccgc tgtcgttcgg ctggtgttgt gcaacgcttc
     1081 gtacacttct ccgctcaccc agagatgatg cagcaacacc gccagctttc ttgccgtggc
     1141 aacgatggct cgcttcttcc cgttccttcc accacgctcc gccagcttca gtccccagcg
     1201 cctgagatcg cagtcgaccc cgaatggtcc caagatgtgc tgcgctccct gcaccagcag
     1261 cgtacgcaga tacggatcgc cctccttgct gatgtgcaac tgcggttcgc tctgtcccga
     1321 gtttctccgt cccggctgca atcccagata acaacctacg tcgcggctcc tgcgaaaccg
     1381 atgcggatct tccagcgtca gcaggaaggt cagtgcgatc agcgtgccca cacctttgat
     1441 ctgtttcagc agcgccacct tgggataact ctgttgcgcc agcgtttcga tgcgttcgtt
     1501 gtactcgccg atccgctggc tcagctcctc gatcgccgac agcaatggct ccagcgcccg
     1561 ttgcagttcc ggacttagcc cttcggcttt ctccggattc atgttgcgca cgttgcagcc
     1621 acgcagccgc tcgccgtaac tcttggccag cccgcgtacg gtgttgacca gccccgttcg
     1681 cgcccgcacc aggccggctc gcgcccggat caccgccaaa tccgcttgcg cctgcgcgct
     1741 gcgatgcttc accggacaca gcaactcggg atcgatccgg gccagtcgcg ccaaggtctg
     1801 cgcatccagg cgatcgtcct ttttccggct ctctccgatc agccgcacct tgcgcgcatt
     1861 cgccacgatc acttcgtgcc ccaactcggc gagcaaacga ctgatccagg gcgaatgcgt
     1921 tccggtctcc agcgcaatcc ggcagtgcgg catcgcgccg aaggcttcct gcaacgcctt
     1981 cgcactcgtg cggactcgct gctccaactg tatctggcct gcttcatcca ccacgcagta
     2041 ccaactgttg cgatctccta gatccaaacc gatggtcagc ttctgctgac aaatattcct
     2101 gctctgcctt gccgccgcgg tgctaacctt cttcattgcc ggtctccttt atctcttgcg
     2161 cttcgagcgc gtcgataact tgggagcgta cagcatcccg tccggagacc ggccttctca
     2221 tcccatctga tttcgccaac gggccgatga ttggtgtcta atgtgaatgt gagaccactt
     2281 gccaacattt tccgcgtagc ggcattcctt gtactgacat t
//
LOCUS       DASTPM010000265         2631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_816578, whole genome shotgun sequence.
ACCESSION   DASTPM010000265 DASTPM010000000
VERSION     DASTPM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816578"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..856)
                     /locus_tag="VFR42_04685"
     CDS             complement(89..856)
                     /locus_tag="VFR42_04685"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0368478.1"
                     /translation="MQYPQFTLSGQVALVTGAARGLGRAIALALAHAGADVALGLRDV
                     QADAGLVAEITKMGRKTLPLQMDMREMEQIFGAVEKTVKEFGRIDILVNNAGIAPENP
                     AEDVREGDYDATVAINLKGTFFASQAAGRIMIRQKSGKIINMSSQAGFAALPTESVYC
                     MTKAGIAHLTKCLAVEWGKHGITVNAVAPTFIYTPGTQGVLADAAFRADTVERIAALH
                     RIGEPMEVAGAVVFLASPAASLITGATILIDGGWTAR"
     gene            1036..1470
                     /locus_tag="VFR42_04690"
     CDS             1036..1470
                     /locus_tag="VFR42_04690"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HEU0368479.1"
                     /translation="MKKLLVVSAVAGLIAAAALAATVRAQKSKKVVYASSAQATYKPS
                     QMKGVSMEAVWGDADKGAHGTFTKFDPGYDAGMHTHTNDVSIVVIKGAYLYKDEAGEK
                     RVGPGDYLFVPGGHKHWSGGDKTEGAVFYEEGSGKFDLVPAK"
     gene            complement(1555..1785)
                     /locus_tag="VFR42_04695"
     CDS             complement(1555..1785)
                     /locus_tag="VFR42_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368480.1"
                     /translation="MSSIRDAAKSNADPVNNTWQKTHDYQLIYDTARNRGYPCRPSDR
                     WLAADSDEHGNREPRPGAGVYRFGESDSVSLP"
     gene            complement(1786..2127)
                     /locus_tag="VFR42_04700"
     CDS             complement(1786..2127)
                     /locus_tag="VFR42_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0368481.1"
                     /translation="MSVTMRDSEVNGVSVVELDGRIVLGEESNSLREKLKSLIAAGKR
                     KIVLNMANVRYIDSAGLGALVAGHVSAKTQGASVRLCNLGQKFYEVLQVTRLMTVFDV
                     YDTEAAAVSSF"
BASE COUNT          548 a          814 c          680 g          589 t
ORIGIN      
        1 aagggcgacg tgcgctttct gtcctttttg cttaaagacg cggcaaaaac ggccctaccg
       61 aatcgcgttt agcgcgtaag cccggccgct accgcgccgt ccagccgccg tctatcagta
      121 tcgtcgcgcc ggtgatcaac gacgctgccg gcgaagccag aaacaccacc gcaccggcca
      181 cttccatcgg ttcgccgatg cggtgcagtg ctgcgatccg ttccaccgtg tccgcgcgga
      241 atgccgcatc cgccaatacg ccctgggtcc ccggtgtgta gatgaatgtc ggtgccaccg
      301 cgttcacggt tatcccatgc ttgccccatt ccaccgccag gcatttcgtc aaatgcgcga
      361 ttcccgcttt ggtcatgcaa taaaccgatt ccgtgggcag ggctgcaaat ccggcttgcg
      421 aactcatgtt gataatcttt ccgcttttct gccggatcat gattcgtccc gccgcttggc
      481 tcgcaaaaaa tgtccccttc aggtttatgg ccaccgtggc gtcataatct ccctcacgta
      541 catcctcagc cggattttcc ggcgcgatcc cggcattgtt caccaggatg tcgattcgtc
      601 cgaactcctt gacggttttc tccaccgctc caaaaatttg ttccatctcg cgcatatcca
      661 tttgtagtgg tagcgtcttt cttcccatct tggttatctc cgcgaccaat cccgcgtcgg
      721 cctgtacgtc gcgtaaaccc aacgccacgt ccgcgcccgc atgcgccagt gccaatgcaa
      781 tcgcccgccc caacccacgc gccgctccgg ttaccagcgc cacttggccg ctcaaagtaa
      841 actgcggata ttgcattact ttcctccgcc cttcggatta attctccgag cgcgtccatg
      901 ttcccataat ctcggagcat tctgaaaacg tgtcggctgg aattctttac ccgcgagcag
      961 agcgttggtg tataactcat ctcaaccctg ggcagttcaa acgcattccc cacctggaag
     1021 tatacggaga gaaaaatgaa aaaattgttg gttgtatcgg ccgttgcggg tctcatcgcc
     1081 gcggcggctt tggcagccac agtacgcgct cagaaaagca aaaaggtcgt ctacgcatct
     1141 tcggcgcaag ccacctacaa accttcgcag atgaagggcg tttccatgga agccgtttgg
     1201 ggcgacgcgg acaaaggtgc tcacggaaca ttcaccaagt tcgatcccgg gtacgatgcg
     1261 ggcatgcaca cgcacaccaa cgacgtttcc atcgtggtca tcaagggcgc ctatctatat
     1321 aaggacgaag ccggcgagaa gcgtgtcggc ccgggcgact acctgtttgt tccaggcggc
     1381 cacaaacact ggagcggcgg cgacaaaacc gaaggcgcgg ttttctacga ggaaggctca
     1441 ggaaaattcg acctcgtacc ggcgaagtag ttgctggcag ggtgactgat tgacacaggc
     1501 aaaccagggc ggctggattg gcaatctcgc cgccactcta ctcaagagag ttacttaagg
     1561 tagggagacg gaatcgctct ctccaaaacg ataaacacct gcacctggtc ggggttcacg
     1621 gttgccgtgc tcatcagaat cggccgccag ccatctgtcg cttgggcggc aagggtagcc
     1681 tcgatttcgg gccgtgtcgt agatcagctg gtagtcatgc gttttttgcc aagtgttgtt
     1741 cacaggatcg gcgttgctct tcgccgcatc gcgaatcgaa ctcatttaga aactgctgac
     1801 cgccgctgct tcggtgtcat acacgtcgaa aaccgtcatc aaccgcgtca cctgcagaac
     1861 ctcgtagaat ttctggccca gattgcagag acgcaccgaa gcgccctgtg tttttgcgct
     1921 gacgtgcccc gccaccagcg cgcccagccc ggcgctgtcg atgtaacgca cgttagccat
     1981 gttcaagaca attttccttt tgccggcggc gatcaggctc ttcaattttt cgcgcagaga
     2041 attgctctcc tcgcccagca cgatgcgtcc gtccagttcc accacggaca cgccattcac
     2101 ttcgctatcc ctcatggtta cggacatgct tgttcctcct cccgctcggc caacccagcg
     2161 ccaggcgggc actccagtag agcacgccgc tacgcgcgaa tgtggaaaga aatacatgtt
     2221 tcttggtcgc cggccggttc gcgcccttcc tgccgcgccc ttccgtggcc caatcccgat
     2281 ggtgtgtcca atcacctgcc gaattctgac agatccagga gttcgttcag agcggcggac
     2341 atccggaatg ccgggcgtga tcggtcaggg catggtcaat ttccagctga tccgttactc
     2401 tcagaaggaa tggaccaaac ggctcattta gctgaccgac gcagacaagg aactggcgta
     2461 ccgctatgag aaaacagata ccatcgcaaa agcgatgtta ggccttcgca aattctctgc
     2521 cattcagctg gcggactata accaattcat aaaaacgcgt ccggaatttc ttgtgtattc
     2581 tgaggttcgg gctggatact cgaccacctg atccgcgagg tagcttcggt t
//
LOCUS       DASTPM010000266         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_758909, whole genome shotgun sequence.
ACCESSION   DASTPM010000266 DASTPM010000000
VERSION     DASTPM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758909"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..2784)
                     /locus_tag="VFR42_04705"
     CDS             complement(145..2784)
                     /locus_tag="VFR42_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0368482.1"
                     /translation="MPEPVHKDSPVGGGSNEPPRSNVADRLAQFEERQRHLWRVTYGL
                     LGLLTLAYVAVSRDAIRNFAQRYEVLLLGLIVLVGTFIFYAWRRNKEIAELRGLVRGI
                     EQRAATPPSDHQLDKLFSVIEKSQQGYRDLIDSFDDLLIAVTLDGEIRAANRSFADLV
                     GASFQEIVGHPLSEFLEDGGGEGPNLLERHMPRFLENRHWYGVIQVRLKKRNTINYFD
                     CVIHAMLRDGQVHGMTILGRDITASRRNEARFTELFETLQEGIYIVTPDDQILDVNPA
                     LVRILGYDSKTELLARKVSEVFPDESLRAVIRHEVDRQPVLEGREITLLRKDGVAITC
                     LNTAAAVRDSSGKIVRYQGALMDISSRREMERRLHKQQEFARRLVDSFPDLILVLDTK
                     SHYTFISPRCRDVLGYEQDDTRDMQFGGRTHPEDLPGLMTLYRDIIRGNQSYSSMEVR
                     VRHKQGDWRRILFNFSPLFDESNKIEGVVLSGRDVTDLKRLEEQLIQAEKLAAMGQML
                     AGVAHELNNPLTAILGVTELVREREGLDDSMKRQLDLTHRQARRAARIVQNLLEFSRP
                     ASPQKKPIDLNSIIERTLQLHEHSLRRNQVAVDFSPRTDLPAVVGDANQLIQVLLNLI
                     SNAEQAIREVRESGRVQIRLASSSGSVIVTVQDDGVGIPADALPKLFDPFFTTKRPGG
                     GTGLGLSICLSIVREHGGTIQAESLPGGGSAFKIYLPVARDVPHGASPSRSTESSDSG
                     SDHDPRPTPSVLKGIRVIVLDDEESIRSLLQEGLRAHGLDVACAATAEEAAALITARP
                     CDALLCDLRLKGASALSDGKAAAAHVLSAAGAQKPVVIFMTGEFVDQNSNLAGASLLQ
                     KPFRILDVLAVMKEALVFAKQGR"
     gene            3034..>3576
                     /locus_tag="VFR42_04710"
     CDS             3034..>3576
                     /locus_tag="VFR42_04710"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368483.1"
                     /translation="MTTEGVASELVPAASAEVNPWLIAVVVSLAAFMEVLDTSIANVA
                     LPHIAGDLGASTDESTWVLTSYLVSNAIILPVSGWFVGIFGRKRFFMLCILIFTVSSL
                     LCGVAWSLGLLLLARILQGAGGGGLQPLAQAILADTFPPEKRGLAFSLYGVTAIVAPS
                     IGPTLGGWITDNYSWRWIFYI"
BASE COUNT          756 a          999 c         1086 g          735 t
ORIGIN      
        1 acgctgggtg aagcgttgcc ggcgggcgag tacgccttcg cgcagatttt gccggacgga
       61 atcaatctgt acgtatggga ttttggggtg gaccgaaaaa gttccagcgg ctcggaaaag
      121 aaaaactgaa ccagaacgct acttttatct tccctgcttg gcgaaaacga gggcttcctt
      181 cattaccgcc aaaacatcga gaatgcgaaa tggcttctga agaaggctgg caccggcaag
      241 attgctgttt tggtcgacga attctccggt cataaaaatg acgacaggct tctgcgcgcc
      301 agccgcagag agcacgtgtg ccgcagcagc cttcccgtca cttaaggcgc tggcgccctt
      361 gagtcgcaag tcgcacagca gcgcgtcgca ggggcgggcg gttatcaagg cagcagcctc
      421 ctcggcggtt gctgcgcacg caacatcaag gccgtgagcg cgcagtccct cttgcagaag
      481 cgagcggatg ctctcttcgt cgtccaacac gatgacacga atacctttca gcacgctagg
      541 tgtggggcga ggatcgtggt cggaacccga atcggacgac tccgtcgagc gtgacgggga
      601 ggctccgtgc ggaacgtcac gggcaacagg cagataaatc ttgaatgctg aacccccgcc
      661 gggtagagac tccgcttgaa tcgttccccc atgttcgcga acgatcgaaa gacagatgct
      721 caaacccagg ccggttccac ctccgggacg cttggtggtg aaaaacgggt cgaacaattt
      781 gggcaaagcg tctgcgggaa tcccaacgcc gtcatcttga acggtaacga tcacactgcc
      841 gcttgaagag gcaaggcgaa tctggacgcg gcccgattcg cgaacttcgc ggatggcctg
      901 ttcggcgttg ctgatgaggt ttaacaagac ctggatcaat tggttggcgt cgcccacaac
      961 cgctggcaga tcggttcgtg gactgaaatc caccgccacc tggttgcggc ggagcgaatg
     1021 ttcgtgaagt tgcagcgtgc gctcaatgat cgaattgagg tcgatgggct ttttctgcgg
     1081 ggaggcagga cgcgagaatt ccagcagatt ctgcacaatg cgcgcggcgc ggcgagcttg
     1141 gcggtgcgtg agatcgagct gccgtttcat ggagtcgtcg aggccctcgc gctcgcgaac
     1201 cagttcggtg acacccagaa tggcggtgag cggattgttc aattcgtgag cgacgccggc
     1261 cagcatctgg cccatggcgg cgagtttttc ggcctggatc aattgctctt cgagacgctt
     1321 caggtcggta acatcgcggc cggaaaggac gacaccttcg atcttattgc tctcgtcaaa
     1381 caacggactg aaattaaaga ggatgcggcg ccaatcgccc tgcttgtggc gaacgcgcac
     1441 ttccatggac gaatagcttt gattgccgcg aatgatgtcg cgataaagag tcatcagccc
     1501 cggcaagtct tccggatgag tccggccgcc gaactgcatg tcgcgcgtat cgtcctgttc
     1561 gtagccgaga acatcacggc agcgtgggct gataaacgta tagtgtgact tcgtatccag
     1621 aaccaggatc agatcgggga agctgtccac caagcggcgc gcaaattcct gctgtttgtg
     1681 cagccgccgc tccatttcac gccgggacga aatgtccatg agcgcgcctt gatagcgcac
     1741 aatttttccg gacgagtcgc gtaccgcggc ggccgtgttc aggcaagtga tcgcaacgcc
     1801 gtccttgcgg agcaaagtaa tttcacggcc ttccaggacc ggctggcggt cgacctcatg
     1861 ccggattacg gcgcgcagcg attcgtccgg aaacacttcg ctgactttgc gggcgagcag
     1921 ctccgtcttg gagtcgtaac cgagaatacg cacgagagcc gggttaacgt caaggatctg
     1981 atcgtcgggc gtgacgatgt aaatgccttc ctgaagcgtt tcgaagagct cggtgaagcg
     2041 ggcttcattc cgccgggaag cagtgatgtc acggccgagg atggtcatgc cgtgaacctg
     2101 gccgtcacgc agcatcgcgt gaatcacaca atcgaagtag ttgatggtgt tgcgcttttt
     2161 caagcggacc tggatgacgc cgtaccagtg gcgattttcc agaaagcgcg gcatatgccg
     2221 ctccagcagg ttcggccctt ctcctccgcc atcttcaaga aactcagata gtggatggcc
     2281 gacgatctcc tggaaggatg cgcccaccaa atcggcaaaa ctacggttgg cggcgcggat
     2341 ttcgccgtcg agagtgacgg caatcagcag gtcatcgaag gaatcgatca gatcacggta
     2401 accctgctgc gatttttcga tgaccgagaa cagcttgtct agctggtgat cgctcggcgg
     2461 agtagcggcg cgttgttcaa tcccccggac caagcctcgg agttccgcaa tttctttgtt
     2521 gcgcctccag gcatagaaaa taaacgttcc aacgagtacg atcagcccta agagcaaaac
     2581 ttcataacgc tgagcgaagt tgcgaatggc gtcccgggaa acggcgacgt aggccagcgt
     2641 cagcaggcca agaagtccgt aggtgacgcg ccacaaatgg cgctggcgct cctcgaactg
     2701 cgccaggcgg tcggcgacgt tagaacgtgg aggctcgttg gagcctccgc ccacagggga
     2761 atccttatgg accggttctg gcatgagcga ttcaataacg cgccagagac gaagctctgt
     2821 gcccgcaggt tcattctccg cctcggccgg agaagcggca attggtagaa ggggcggaag
     2881 ttcctgagcg gcacctgtac ggacgatatg cgccaaccag cgccgcggat aggccagacc
     2941 tcaggtcgag gcggggtcaa tttggcgtcc ctgcggaaaa cgggctaaac tcgaccgcca
     3001 ccgcatgata attccagaga gtcctttcca tccatgacca cggaaggagt tgcgagcgag
     3061 ttggtgcccg ccgcaagcgc ggaagtgaat ccgtggctca tcgccgtggt ggtttcgctc
     3121 gccgccttca tggaagtgct ggacaccagc atcgccaacg tggcgcttcc gcacatcgcc
     3181 ggagatctcg gggccagcac cgatgaaagc acctgggttc tgacttcgta cttagtttcc
     3241 aacgcgatca tcttgccggt gagcggctgg tttgtcggca ttttcggccg caaacgcttt
     3301 ttcatgctgt gcatcctgat ttttacggtc agttccctgc tatgcggagt tgcctggagc
     3361 ctcggcttgc tgctgttggc acgaatcttg caaggcgcgg gcggaggagg actgcaacca
     3421 ctggcgcagg cgatccttgc ggataccttt cctcccgaaa aacgcgggtt ggcattctca
     3481 ctttatgggg tgaccgctat cgtcgcgcct tccatcggcc ccacgctcgg cggatggatt
     3541 accgacaatt attcgtggcg ctggattttc tatatc
//
LOCUS       DASTPM010000267         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_326540, whole genome shotgun sequence.
ACCESSION   DASTPM010000267 DASTPM010000000
VERSION     DASTPM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326540"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFR42_04715"
     CDS             <1..686
                     /locus_tag="VFR42_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0368484.1"
                     /translation="ESIEIIEAMLAGDEVKYEGKAFQAFAPALKADTHVPRWRVPIYV
                     GATGPVLQRLAGSHSDGLLTASITTPKFVQYSRKMMEEGARKAGKETAKLDLGSVIVG
                     SVGENPKTAREGAREMAAMYLANKVQNIRGSADTLLECAGLAFDEIRPIAEAMEQGGR
                     KAAARAVTDAVLQKVCAIAGTPAQCVERLEEYRAAGCTHIMLELWGDDRLEQARLFGE
                     KVIPHFKRK"
     gene            complement(701..1906)
                     /locus_tag="VFR42_04720"
     CDS             complement(701..1906)
                     /locus_tag="VFR42_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368485.1"
                     /translation="MNEKPDGIWVSELNNRPEVRKAFDSTRAVQFYDTTLRDGEQSVG
                     VVLTPQQKLEIARALDALGVGRIEAGFPRVSADDAEAFGLIAKAGLKAEIWGFGRALK
                     ADIDELLRLGAHATVIETPTSAIKLKAYGLSIDDAKRRAAEAISYASKNGVTVAFFTV
                     DGTRTDLDVLRDFYRCALDAGAQELVVVDTIGACGPEAVEFLVRQVREWVGIKIPLHF
                     HGHNDFGMATACAVAAVRGGAQWIQGTINGMGERAGNADIGEVAMALKGLYGVPVALD
                     LPKIREVSKLVRRIAGHEMEPWKPVVGENLFIRESGAVASQFHIPEAIEPFSATLVGA
                     TRSIVLGKKSGIDSIAIKAKELSISLTTEEQQAALAAVKRAGTEKRGLVTDHEFRAIT
                     ERVKRAATA"
     gene            complement(1933..>2208)
                     /locus_tag="VFR42_04725"
     CDS             complement(1933..>2208)
                     /locus_tag="VFR42_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0368486.1"
                     /translation="VNGKARQQSHSSKMSVTVPEIIAHYSPMGYSAGDVLSTGTVSGV
                     AAFSGDPKSLYLRPGDVVECEIEKIGVLRNPVVSYREKHGGKTVAAA"
BASE COUNT          426 a          622 c          687 g          473 t
ORIGIN      
        1 gggaaagcat cgagattatt gaagcgatgc tggcgggcga tgaagtgaag tatgaaggca
       61 aagcgtttca ggctttcgcg ccggcgttga aagcggatac gcacgtgccg cgctggcggg
      121 tgccgattta tgttggggct accggcccgg tgttgcaacg gctggctggt tcgcattccg
      181 atggcctgtt gacggccagc atcactacac cgaaatttgt gcagtattcg cggaagatga
      241 tggaggaagg cgcgcgcaag gccgggaaag agaccgcgaa gctggatctc ggctcggtga
      301 ttgtcggcag cgtgggagaa aatccgaaga cggcgcgcga gggcgcgcgt gaaatggcgg
      361 ccatgtacct ggccaacaag gtgcagaaca ttcgcggatc tgcggatacg ctgctggaat
      421 gcgcgggact tgcgttcgat gagattcgtc cgattgccga ggcgatggaa caaggcggaa
      481 ggaaggccgc agctcgcgcg gtgaccgatg cggtgctgca aaaagtctgc gcgattgccg
      541 ggactccggc gcagtgcgtt gaacggctgg aggagtatcg cgcagcgggc tgcacgcata
      601 tcatgctgga attgtgggga gacgaccggt tggagcaggc gcggctgttc ggggaaaaag
      661 taattccaca tttcaagcgg aaatgattcg gcgaaaaggc ctaagcagtg gctgcccgtt
      721 ttacgcgctc ggtgatagcg cgaaattcgt ggtcggtcac caggccgcgc ttttccgttc
      781 cagcgcgctt cacggcggcg agcgccgcct gctgctcctc ggtggtcagc gaaatactca
      841 gttctttggc tttgatggcg atgctatcga ttccgctttt ctttcctaac acgatcgagc
      901 gggtggcgcc gaccaacgtt gccgaaaatg gttcaattgc ttcgggaatg tgaaactggc
      961 tcgccacggc tccgctttcg cgtataaata aattttcgcc gacgactggc ttccatggtt
     1021 ccatctcatg accggcaata cggcggacca atttagaaac ttcacgaatc ttgggcaagt
     1081 ccagcgctac gggcacgccg tagagtccct tcaatgccat ggcgacttcg ccgatgtcgg
     1141 catttccggc gcgttcgccc attccgttga tggtgccctg aatccattgc gcaccgccgc
     1201 gcaccgcggc cacggcgcag gcggtggcca ttccaaaatc gttgtgcccg tgaaaatgca
     1261 gcgggatttt gatgccgacc cattcgcgca cctggcggac gagaaattcc accgcttccg
     1321 ggccgcaagc gccgatggtg tcgacgacga ccaattcctg cgcacccgcg tccagggcgc
     1381 agcggtaaaa atcgcgcagg acgtctagat cggtccgcgt gccatcgacc gtgaaaaacg
     1441 ccacggtgac tccgttcttg ctggcgtagg aaatggcttc ggcggcgcgg cgtttggcgt
     1501 catcgatact caggccgtag gctttgagtt tgatcgcgct ggtaggcgtt tcgatgaccg
     1561 tggcgtgcgc gcccaagcgc aataattcgt cgatatccgc tttgagggca cgcccgaatc
     1621 cccaaatttc ggccttgagg ccggctttgg ctatcaggcc gaatgcttcg gcatcgtcgg
     1681 cggaaacgcg tggaaagcct gcttcgatgc gccccacacc caatgcatcc agcgccctcg
     1741 cgatttccag cttctgttgg ggcgtcagga caacgccgac gctttgctcc ccgtcgcgca
     1801 gagtggtgtc gtaaaactgg acggcgcggg tggagtcaaa tgctttgcgc acttccggcc
     1861 tgttgttcag ttcgctgacc cagatgccat ctggtttctc gttcatagtg aattttcaaa
     1921 tttcaacttt gttcacgccg cggccaccgt ttttccgccg tgcttctcgc ggtacgagac
     1981 caccgggttt cgcagcacgc cgattttttc aatctcgcac tccaccacat cgccgggcct
     2041 caagtacagg gatttgggat cgccgctgaa cgctgcgacg ccggaaaccg ttccggtgga
     2101 cagcacatca cccgccgaat agcccatcgg cgaatagtgc gcgatgattt ccgggacggt
     2161 gaccgacatt ttgctggaat gcgactgttg ccgggctttt ccgtttac
//
LOCUS       DASTPM010000268         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_153330, whole genome shotgun sequence.
ACCESSION   DASTPM010000268 DASTPM010000000
VERSION     DASTPM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153330"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VFR42_04730"
     CDS             <1..1207
                     /locus_tag="VFR42_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368487.1"
                     /translation="PSAAAEVSSDPDSGPAPSSTSAKNTPEAASLPPANECLPSLSAK
                     TGRDFWAPHELEESVPHVDPTVACPSDDVLRGVGARMTEFVQIMERFTATEKLEHFPV
                     DRKGVRKSPETRSFAYVVSVTQNSMGTFLLDEFRDGRMDPEQFPAHTASRGSPAMALI
                     FHPILASDFEFRCEGLGLWAGREAWQVHFVQRPDHPIRIRSYSVGGRSVGLALEGRVW
                     IDPGSYQVLRLESELAKSLPEIALTLEHFTIDYQPVRFQSTGQQLWLPQVAEIYVERK
                     NKRFYRRHVFSDFRLFNVDTSQKIRTPSESFSFTNTSDRDIGGELTIERNNSSIGGPV
                     TLRFVVPAHSVIFKIVGPGKDINIPPSTVASATFTHEGEAAAIKVDTRLAKETAVDVV
                     PQTSVSPQP"
     gene            1360..2685
                     /locus_tag="VFR42_04735"
     CDS             1360..2685
                     /locus_tag="VFR42_04735"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0368488.1"
                     /translation="MATLLLSPLPVAQSQSPGAILEGKVCNSTGDPVASVLIQLKKES
                     GEVIASTQTNAAGQYRFSSLQAGSYRLTLTSDSFRALELSAGSLKTGEAKRLDATLEK
                     VTNAASSPGAGPLEFSDAPAFTVAGVTDWSNVGLHGSDANVHTSDALAKETASLKSSS
                     PARVTGASRAGDAHRSLGDAKESSGDPVGAVNEYAAAVKADPSEQNYFAWGAELLLHR
                     AGQPAVQVFSAGSKAHPDSSRMLAGLAAAFYSNGQYNEAATEICRASALSPADSAPYL
                     FLGKMEQAAADPFSCSEKTLARFATDQPANAQANFLYGLVLWKRARKTQDYSGMAHAE
                     RLFQKSAGLDPHFADVYLQLGMLYNAQGKKDAALGAFQKAAAADPEFSAAHYQLSLAY
                     RRSGNAAKADEEMKTYDELRRSEAVAFEKERHELRQFVTTLKDEHASPR"
     gene            2792..>4701
                     /locus_tag="VFR42_04740"
     CDS             2792..>4701
                     /locus_tag="VFR42_04740"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEU0368489.1"
                     /translation="MNSFWRKVTDAVLLAALLAPISYAADSSFTLRSPDDRIEVQIHV
                     ANRITYDVALNGKPLLKDSSLSINIAGKTLGENPQLQSTKKNSVDKMLEPVVRQKFAK
                     IREHYNELRLDFDGGYAVVFRAYPEGVAYRIETSLGGENVKVFSEDATFRFVSDFSVF
                     YPAEESVFSHNERKYLPRPLKEISDKTFATLPAVVDANGVKIAVAESDVEEYPGLWLR
                     GTGDNALAASFEHYPLKETLEKDRDFKVTEAADYIAVTKGTRTFPWRVLGLAERDRDL
                     ISNSLIWLLEKPSQLSDTSWIKPGKVAWDWWNYNNIYGVDFKAGINTQTYKYYLDFAA
                     QNHLDYIILDEGWYKLGNVLDVVPEINMEELTGYAKQKKVGVILWVVWKSLADQMQPA
                     LDLYAKWGIKGIKVDFMQRNDQLLMNFYHQLSAECAKRKMLVDFHGAQRPASLTRTWP
                     NLISTEGVRGMEWSKWSAEAEPTHNVTLPFTRMFLGPMDYTPGAMLNAQKDMFAPIMK
                     RPMALGTRCQQLAMYVVYESPLQMLSDSPSNYEREPESLEFLRAVPSAWDETVPLDGK
                     ISEHVVVARRSGKDWYVGAMTNWTARDIEIDLSFLPAGNFTLDAYQDGINADRMASDY
                     KKTTSPVTKSTKL"
BASE COUNT         1011 a         1589 c         1143 g          958 t
ORIGIN      
        1 accttccgcc gcggcggagg tttcttctga cccggattct ggccctgcgc caagttctac
       61 ctccgcgaag aacactcctg aggccgcctc cctacctccg gccaatgagt gtttgccttc
      121 gctgtccgcg aagactggcc gcgatttctg ggcgccccac gagctcgaag aatcggtacc
      181 ccacgtggat cccacggtgg cttgtccgtc ggacgacgtc cttcgcggtg tcggcgcacg
      241 catgacggaa ttcgtgcaaa tcatggagcg cttcaccgct actgaaaagc tggagcactt
      301 tccggtggat cgcaaaggcg tccgcaagtc gcccgaaacc cgttcgttcg cctatgttgt
      361 ctcggttacc caaaatagta tgggcacttt ccttctcgac gaattccgcg acggcagaat
      421 ggaccccgaa caatttcccg cgcacactgc ttcgcgcggt tctcccgcta tggcgctgat
      481 ttttcatcca attcttgcct ccgactttga atttcgctgc gaaggtctgg gcctttgggc
      541 cggacgtgaa gcctggcaag tccatttcgt ccagcgcccc gaccacccga ttcgtattcg
      601 ttcctactct gtcggcggcc gctccgtcgg cctggcgctt gaggggcgcg tttggatcga
      661 tcccggcagt tatcaggtct tgcgtctgga atccgagctc gcaaagtccc ttccggaaat
      721 tgccctgacc ctcgagcatt tcaccatcga ctatcagccc gttcgcttcc agtccaccgg
      781 ccagcaactc tggcttcccc aggtcgccga gatttatgtc gaacgaaaga acaaacgctt
      841 ttatcgccgc cacgtctttt ccgatttccg tctttttaac gtcgacacct cgcagaaaat
      901 ccgcacgccg agcgagtcct tcagctttac caatacctcg gatcgcgaca ttggtggcga
      961 attgaccatc gaacgcaata actcctcgat cggaggtccg gtcactctcc gcttcgtggt
     1021 tcccgcgcat tccgtcattt tcaaaatcgt cggccccggg aaggacatca acatcccgcc
     1081 ttccaccgtc gcatccgcca cctttacgca cgaaggcgaa gccgccgcca ttaaagtgga
     1141 cacccgcctg gccaaagaaa ccgccgtcga tgtagtcccg caaacctccg tctccccgca
     1201 accttagctg ggattcattt ccttccccgc gattcgaaat gcgcgctcct cattgcggct
     1261 cgccgaccca cagtttagac tgcgaactgc cccatgctca tcagcctttc acagtcggcg
     1321 cgccctcgcg ggaaatgctt tgccttttca gtttctctta tggctacgct gctcctgtcc
     1381 cccctgccgg tggctcaaag ccaatcgcca ggagcaattc tcgaaggcaa agtctgcaac
     1441 tcaactggcg accctgtcgc cagcgttttg attcagctca agaaagagtc cggcgaagtg
     1501 atcgcgtcaa ctcagaccaa cgcggcgggt caatatcgct tttccagttt gcaagccggc
     1561 tcgtatcgtt taacgctgac cagcgattcg tttcgcgctc tcgaactatc ggcagggtcg
     1621 ctaaaaaccg gcgaggccaa gcgcttggat gcaaccctcg agaaggtcac aaacgccgcc
     1681 agcagccccg gcgccggccc actggaattt tctgacgctc cagccttcac agtcgccggc
     1741 gtcaccgatt ggagcaacgt cggcctgcac ggctccgacg ccaacgtcca caccagcgac
     1801 gcgctcgcca aggagaccgc ttctctaaaa tcttcctcgc cggcaagagt gaccggcgcc
     1861 tcgcgcgccg gtgacgcgca ccgctccctc ggcgatgcca aggagtccag cggcgatccg
     1921 gtcggcgcgg tcaacgagta cgccgccgcg gtgaaagccg accccagcga gcaaaattat
     1981 ttcgcctggg gcgccgagct tcttctccat cgcgctggcc aaccggcggt tcaagttttc
     2041 tccgccggct cgaaagccca tcccgattca tctcgtatgc tcgctggcct ggccgccgct
     2101 ttttattcga atggccagta caacgaagcc gccacggaaa tctgccgcgc ctcagctctc
     2161 agccccgccg attccgctcc ctatctcttc ctcggaaaaa tggaacaagc cgccgccgat
     2221 cccttctctt gcagcgagaa gacactggct cgctttgcga ccgatcagcc cgccaacgcg
     2281 caagccaact tcctttatgg tttggtcctt tggaaaagag cccgcaaaac tcaagattac
     2341 tcgggcatgg cccatgccga gcgcctcttt caaaaatccg ccggcctaga cccgcacttc
     2401 gccgacgttt acctccaact cgggatgttg tataacgcgc agggcaaaaa ggacgctgcg
     2461 ctcggcgcat ttcagaaagc cgcggccgct gatccggagt tcagcgctgc tcactaccag
     2521 ttgagcctcg cttaccgccg ctcagggaac gctgcgaaag ccgacgagga aatgaaaaca
     2581 tacgacgaac tgcgtcgctc tgaagccgtc gcctttgaaa aagagcgcca tgaacttcgc
     2641 cagttcgtca ccaccttgaa ggatgaacac gcttcgccgc gatgaacgtt ggatttcgtg
     2701 cgtttttcat gccgcgaaaa gagtttggct gcctgtcatt ccgagcgtcg tcgaggaacc
     2761 tcggcaacgt gcgaacacaa aggatcaacc tatgaactcg ttttggcgca aagtcaccga
     2821 cgctgtactt cttgcggctc tgctggcccc gatttcatat gccgccgact cgagtttcac
     2881 cctccgctcg cccgacgatc gcatcgaagt ccaaatccac gtcgccaatc gcattaccta
     2941 cgacgtcgcg ctcaacggta aaccgctcct caaagactcc tcgctctcca tcaacatcgc
     3001 cggcaaaacc ctcggcgaga atcctcaact ccagtccacc aaaaagaatt ccgtcgacaa
     3061 aatgctcgag ccggtcgtcc gccagaaatt cgcaaaaatc cgcgaacact acaacgaact
     3121 tcgcctcgac ttcgacggcg gctacgccgt agtcttccgc gcctatccgg agggcgttgc
     3181 ctatcgcatc gaaacctctc tcggtggcga aaatgtaaag gttttcagcg aggatgctac
     3241 cttccgcttc gtctccgatt tctccgtctt ctaccccgcc gaggaaagtg tgttctccca
     3301 caacgaacgc aaatatcttc cgcgaccgct caaggaaatt tccgacaaaa cttttgctac
     3361 actccccgcc gtcgttgatg ccaacggcgt caaaatcgcc gtcgccgaat ccgatgtcga
     3421 ggaatatccc ggcctctggc tgcgcggtac cggcgacaac gccctggccg cctccttcga
     3481 acactatccc ctcaaggaaa ccctcgaaaa agaccgcgac ttcaaagtca ccgaagccgc
     3541 cgactacatc gctgtcacga aaggcacgcg cacgtttccc tggcgcgtac tcggcctggc
     3601 cgaacgcgat cgagacctca tctccaattc gctcatctgg ctcctcgaaa agccctcgca
     3661 gctcagcgac acttcctgga tcaaaccggg caaagtcgcc tgggactggt ggaactacaa
     3721 caatatctac ggcgtcgact tcaaagccgg catcaacacc caaacttaca agtactacct
     3781 cgacttcgcc gcccaaaatc atctcgacta catcattctc gatgaaggtt ggtacaagct
     3841 tggcaacgtt ctcgacgtgg ttcccgaaat caacatggag gagctgaccg gctacgccaa
     3901 acagaaaaaa gtcggtgtaa tcctttgggt agtctggaaa tccctcgccg atcagatgca
     3961 gcccgccctc gatctgtacg ccaagtgggg aattaaggga atcaaagttg acttcatgca
     4021 gcgcaacgac cagctgctga tgaatttcta tcatcagctc agcgccgaat gcgccaaacg
     4081 caaaatgctt gtggacttcc acggtgccca gcgtcccgct tcgcttaccc gcacctggcc
     4141 caatctcatc agcaccgaag gcgtacgcgg catggaatgg agcaagtgga gcgccgaagc
     4201 cgaacctacg cacaacgtca cgctcccgtt cacgcgaatg tttcttggcc ccatggacta
     4261 cacgcccggc gccatgctca acgcgcaaaa agacatgttc gcgccaatca tgaagcgccc
     4321 gatggcgctc ggcacccgct gtcagcaact cgccatgtac gtcgtctatg aaagcccgct
     4381 gcaaatgctt tccgacagcc cttcgaacta cgagcgtgaa cccgaatcgc tcgaatttct
     4441 ccgtgccgtc ccctctgcct gggacgaaac cgttccgctc gacggaaaaa tttccgaaca
     4501 tgtagtcgtc gcccgccgca gcggtaaaga ctggtacgtc ggagccatga ccaactggac
     4561 cgcgcgcgac atcgaaatcg atctgtcgtt cctgcctgcc ggcaacttta ccctggacgc
     4621 ctaccaggac ggcatcaacg ccgaccgcat ggccagcgac tacaaaaaga ccaccagccc
     4681 ggtcaccaaa tccaccaaac t
//
LOCUS       DASTPM010000269         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1364430, whole genome shotgun sequence.
ACCESSION   DASTPM010000269 DASTPM010000000
VERSION     DASTPM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364430"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFR42_04745"
     CDS             <1..462
                     /locus_tag="VFR42_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEU0368490.1"
                     /translation="EQAARAANAGGLPPDLSLIAKARSYERGFPNFIFDFFTQFQEQG
                     PNYVSAILQGFEDKPPAGVTIPDGTYYNKYFPGHAIKMPKPLSDGQVQFDDGSPATLP
                     QYAHDVTTFLMWAAEPKMEARKQLGFQVFVILLIFAFLMYFTKKKVWADAH"
     gene            624..1298
                     /locus_tag="VFR42_04750"
     CDS             624..1298
                     /locus_tag="VFR42_04750"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0368491.1"
                     /translation="MGTVITFKRPDGNEASGYLANAARGNAPGVVVIQEWWGVQDQIK
                     GMCERFARAGFDALAPDLYKGTVVPYHDTSKAEKEMNSLDFMDATTQTVRGAAQYLAR
                     NGARVGLTGFCLGGAVTIIGATKIPELAAGVVFYGIPPEAAAKPADVRIPLQAHFASK
                     DDWCTPALVDAFEKGMKAAGKSLELHRYDAEHGFANEQRMAVHDRQAAELAWGRATEY
                     FAKHLG"
     gene            1477..1860
                     /locus_tag="VFR42_04755"
     CDS             1477..1860
                     /locus_tag="VFR42_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368492.1"
                     /translation="MAITFTRVKDYLNAIANNANLDAGNAGHRVFWNTDYNSFFRGNV
                     PSKSCNAVPVPIINQADKVNSAFFLILKANWCTSPVMPQMPRTGPFVTDNGYTVQLPD
                     KSTVTGAQILTDIENWLKAGAPENG"
     gene            1853..>2427
                     /locus_tag="VFR42_04760"
     CDS             1853..>2427
                     /locus_tag="VFR42_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368493.1"
                     /translation="MASLRCGASRRFFALLVGAFLGLSGAAYADEDCPAPKTWFQAKG
                     TPVPPTSEPKRGDDCEFYQRAWQTFLYSTDKKGDQPRVLTPPYKSYLQVFGTDVPAGL
                     VLPVASKTVAATSNMLVLAPRNMEVARVTEAEDVFQAGSSAILIDQNGRPIFYNIIMN
                     PQFVNFITDKKNSYTDVQKLRAAPATQELPVG"
BASE COUNT          515 a          724 c          706 g          482 t
ORIGIN      
        1 gagcaggcgg cgcgcgccgc caatgccggc ggcctgccgc ccgacctgtc gctgatcgcc
       61 aaggcgcggt cctatgagcg cggcttcccg aatttcatct tcgacttctt cactcaattc
      121 caggagcagg ggccgaacta cgtctccgcg atcctgcaag gcttcgaaga caagccgccg
      181 gcgggcgtca ccattcctga cgggacctac tacaacaaat atttccccgg ccacgccatc
      241 aagatgccga agccgctctc cgacggccag gtgcagttcg atgacggctc gccggcgacg
      301 ctgccgcaat atgcccacga cgttaccacc ttcctgatgt gggcggcgga accgaagatg
      361 gaggcgcgca agcagctcgg cttccaggtg ttcgtcatcc tgctcatctt cgccttcctg
      421 atgtatttca cgaagaagaa ggtctgggcc gacgcgcact gatccgcgaa gtcgacatga
      481 attgaaaaag cccccgaaat gggggctttt ttgttttcgt caccgtcccg gcgacgcatt
      541 cgggaacgac ggcgtgtgat ttgcgtctat gatgtgccgc ggccaaaatc gccgcatttc
      601 aacgaagagc gccggaggaa accatgggca ccgtcatcac tttcaagcgt ccggatggca
      661 acgaagcgtc cggatatctc gccaatgccg cccgcggcaa cgcgccgggc gtcgtggtga
      721 tccaggaatg gtggggcgtt caggaccaga tcaagggcat gtgcgagcgt ttcgcgcggg
      781 ccggcttcga tgcgctggcg cccgatctct acaagggcac cgtggtgccg tatcacgaca
      841 cgagcaaggc cgaaaaagag atgaactcgc tcgacttcat ggacgccacc acgcagaccg
      901 tgcgcggcgc cgcgcagtat ctcgccagga acggcgccag ggtcggtctc accggcttct
      961 gccttggcgg cgccgtgacc atcatcggcg ccaccaaaat tccggagctc gctgccggcg
     1021 tggtgttcta cggtataccg ccggaagcgg ccgcaaagcc cgcggatgtg agaattcccc
     1081 tgcaggcgca tttcgcgagc aaggacgact ggtgcacgcc ggccttggtc gacgccttcg
     1141 agaagggcat gaaagccgcc ggcaaatcgc tagagctgca ccgctatgac gccgagcacg
     1201 gcttcgccaa cgagcagcgg atggccgtgc acgaccgcca ggccgccgag ctcgcctggg
     1261 gccgggcgac ggaatatttc gcgaagcatc tggggtaggg gcggcggcgg cggccgctgg
     1321 cctctcgcat ttttttggcc atggcctcct gaaatctagg ttcgacccgc ttaacggacg
     1381 ggcattccct attattgcta cttgtggtaa tgctcaactg agaagtacca tcttccccgc
     1441 aatgcatttc ctaattgtat atagtggaga cggtcgatgg ctatcacctt cactcgggta
     1501 aaagattatc tcaacgcgat cgcaaacaat gcaaaccttg atgccggcaa tgccgggcat
     1561 cgggtattct ggaatacgga ctacaattcg ttttttcgcg gtaacgtgcc ctcgaaatca
     1621 tgcaacgccg tacccgtgcc gatcatcaac caggcggaca aagtaaattc ggcattcttt
     1681 ctcatcttga aagcgaactg gtgcacgtcg ccggtaatgc cgcagatgcc gaggaccggg
     1741 cctttcgtca ccgataacgg gtacaccgtg caattgccag acaagtcaac ggtcactggc
     1801 gctcagattt tgacggatat tgagaattgg ctcaaagccg gagcgcccga aaatggctag
     1861 tctgcgctgc ggcgcctcgc gtcgtttttt tgcgctcctc gtcggcgcct tcctcggact
     1921 atcgggagcc gcctacgccg acgaggattg tccggctccg aagacctggt ttcaagctaa
     1981 aggaacgccg gtaccgccga cgtccgagcc gaagagaggg gacgattgcg agttttacca
     2041 acgcgcctgg caaacgttct tatattcaac cgataaaaaa ggtgaccaac cgcgcgtcct
     2101 tacgccgccc tataagtcat acttgcaagt gttcgggacc gacgtgccgg ccgggctggt
     2161 gcttcctgtg gcgtccaaaa cggttgccgc cacgtcaaat atgctcgttt tggcaccaag
     2221 gaatatggag gtggcgcggg tcaccgaagc tgaagacgtt tttcaggccg gttcaagtgc
     2281 gattttgatc gatcaaaacg gcaggccgat cttttacaac atcatcatga atccgcaatt
     2341 cgtgaatttc atcacagata aaaagaacag ctatacggat gttcagaagc ttagggccgc
     2401 gccagcgacc caagaattgc ctgtggg
//
LOCUS       DASTPM010000270         4595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_66889, whole genome shotgun sequence.
ACCESSION   DASTPM010000270 DASTPM010000000
VERSION     DASTPM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66889"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFR42_04765"
     CDS             <1..565
                     /locus_tag="VFR42_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0368494.1"
                     /translation="RAEAVDGGFLWNQGARVDEKREFTLKNLAEGELRLAVYGAAKDC
                     YVKQITFGQTLAKDDVISVVKGSNPVLEITLSSRGARVQGAVTDTDGLPAAGVWVVAV
                     PDEARRGNFRLFKSQTTDQYGKYDLHGLAPGSYRIFAWAGIEQGEWEDPEFLRPQEAK
                     GELLELQDEDAKTVNLKMIEKKSLGPE"
     gene            complement(587..856)
                     /locus_tag="VFR42_04770"
     CDS             complement(587..856)
                     /locus_tag="VFR42_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368495.1"
                     /translation="MPIKELASRLSQQPPAMAGVRLRNAQTLHGQLRDSGEVKTLRAV
                     QELSDPGKTPAEDAKEILENIGLVLAGMVTFPLMLLAVLTGWDGR"
     gene            complement(957..1145)
                     /locus_tag="VFR42_04775"
     CDS             complement(957..1145)
                     /locus_tag="VFR42_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368496.1"
                     /translation="MRSKLSFIALARRSNSIVWEEPNPRGVGNVALSIALYDELTVTV
                     AQQDGRLLPGENRSGARE"
     gene            complement(1333..1605)
                     /locus_tag="VFR42_04780"
     CDS             complement(1333..1605)
                     /locus_tag="VFR42_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368497.1"
                     /translation="MSSWQKSDWRQFYEIARRPWRRHRPPRPVYPSGLNRVAPAQGFS
                     LSELDDAGVDLDLAETLGLPVDAGRIGVYGPNVSVLRDFIRSSRPL"
     gene            complement(1900..2265)
                     /locus_tag="VFR42_04785"
     CDS             complement(1900..2265)
                     /locus_tag="VFR42_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368498.1"
                     /translation="MARTLAGAIWSSTKLVPRSNAAAVVAGAVSAAVAAEAVVDMAAV
                     VAAGAAAATVEIAATGAIAAIAGSDFLLPDSCKKRLFIVDAASPFTTLDARALFKSAP
                     SIGPRSRSSLRCLRDVTVS"
     gene            2763..4382
                     /locus_tag="VFR42_04790"
     CDS             2763..4382
                     /locus_tag="VFR42_04790"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU0368499.1"
                     /translation="MRGTMMDFPLTLVTLLERAGKLFSDVEILSRGPDRSVVRTNYGT
                     FYKRARRLASALTKLGLQPGDRVASMMWNHVGHLDAFFGVPCAGGILHTLNLRLHPHE
                     IAAIAQHARDRFLLIDDVLLPVLEEFRADAPFENVIVAPYGCGGAVPDGFLNLEELLA
                     EAGDDFKYPAIDENAGAAMCFTSGTTGFSKGVIYSHRSLVLHSMAECMVDAFALSNND
                     TVLPVAPMFHANAWGVPYTCVMTGAKLMLSGPNVEPEGLLDWMVEERATFVSGVPTVW
                     IGVLDTLEKNPGRWKFDWPVRIICGGTAPPVELIRKLDRFGLQLIHLWGMTETAPLAT
                     TGRLKANMRDWPEEKKYEVRARQGWPVPFLELRTMRPEGEAPWDDETPGEIEVRGPWV
                     AAEYFESPDQAHRWTADGWFKTGDVATIDRDGIVKIVDRAKDLVKSGGEWISSLDLEN
                     TLMGHPAVKEACVVGVPHPKWQERPLAAIVLKDGKAASPEELRGFLAATFAKWQLPDA
                     FVFLDAIPRTSVGKFKKIALREQFADWEWES"
     gene            complement(4410..>4595)
                     /locus_tag="VFR42_04795"
     CDS             complement(4410..>4595)
                     /locus_tag="VFR42_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368500.1"
                     /translation="YEYLRAKGIKTQEPQTAPYGMRQLYLLDPDGYRLCLQWPASDEM
                     RAEWKKRYGSSVVSASG"
BASE COUNT          997 a         1217 c         1514 g          867 t
ORIGIN      
        1 acgggcggaa gccgtcgatg gcgggtttct gtggaaccag ggagcgcggg tggatgagaa
       61 acgggagttc acgctgaaga atctggccga aggcgagttg cgcctggcgg tttacggcgc
      121 agcgaaagac tgttacgtaa agcagattac gtttgggcag acgctcgcga aagacgacgt
      181 aatcagcgtc gtcaagggaa gcaatccggt gctggagata acgctaagtt cgcgcggcgc
      241 gcgggtgcaa ggagccgtga cggataccga tgggttgccc gccgcgggcg tgtgggtggt
      301 agcggtgccg gatgaggcgc gacgcggcaa ttttcgttta ttcaaatcac aaactacaga
      361 ccaatacggg aaatacgatc tgcacgggct cgcgccggga agctacagga tcttcgcctg
      421 ggccgggatc gaacaaggtg aatgggagga tccggaattt ttgaggccgc aggaagcgaa
      481 gggcgagttg ctggaattgc aggatgaaga tgcgaagacg gtgaatctga aaatgattga
      541 gaagaagagc ctgggcccgg agtgagcgcc cgggcgcttg cggccttcag cggccgtccc
      601 agccggtgag cacggccaat agcatcaacg gaaaagtcac catgccggcc agcaccagtc
      661 cgatgttctc aagaatttct ttggcatctt cagcgggagt tttgccggga tcggaaagct
      721 cctgcacagc acggagtgtt tttacctcgc cggaatcgcg cagctggccg tgcagcgtct
      781 gtgcattgcg aaggcggacg ccagccatcg cgggcggctg ctgactgagg cgcgaggcca
      841 actctttgat tggcacgggg atcgccggcg tgtttgggat ccgtccagcg caaggaccga
      901 acgtcggcaa aggaatgctg accgtctgag gagagaattg tatcttgagt gtgaagtcat
      961 tcgcgcgcac ccgatcgatt ctcgcccggg agaagccggc catcttgctg ggcaaccgtg
     1021 acggtcagtt cgtcgtacag agcgatggag agggcaacgt tgcctacgcc gcgaggattt
     1081 ggctcttccc agacaataga gttggagcga cgagccaggg cgatgaaaga aagtttcgat
     1141 cgcatctgca actccaaaga cggcttggtt cgagtagtgc agccgggctg ccgtaacact
     1201 ggggcggatg gagcatcgca atcgcgcgaa aatgcgaagc ctggtcggtt agtaaccgca
     1261 aaagaagtca ctggccgcag gttggccgga ccgaagtgta ctgaatttta gacaggctaa
     1321 ggacggacga gactagagcg gacgagagga gcggatgaaa tcgcggagga cggagacgtt
     1381 ggggccgtac acgccgatgc gaccggcatc gacggggagg cccagggttt cggcgagatc
     1441 gagatcgacg ccggcgtcat cgagttcgga aaggctaaag ccctgcgcgg gcgcaacgcg
     1501 gttaagcccc gaaggataca cgggacgagg cggccggtga cggcgccagg ggcggcgagc
     1561 aatttcataa aattgccgcc aatcgctttt ttgccaggag ctcataccaa tatccatgcc
     1621 aagccttgcg tttccctttc gtgttccgcg ccagccatgc tcacgtctgc actacggctg
     1681 cttgcgaaga ttggatgctc atccttacca aaaggttcac cgatgtcact accaggcatt
     1741 ccgcaaaggc cgggcggaac gtccggtagg cggaaacctt gccgcggcgc agcgccaaga
     1801 tactgcaaaa cgcaaggaac cggagacttt ccttgcgtaa tgtttcaaac ccgatactgc
     1861 gggaaaagtt tcgcagaacg agcgggccca aaaacgagcc tacgaaacgg tgacatcacg
     1921 gagacaacgc agcgaagaac ggctgcgcgg accgatcgag ggggcggact taaacaaagc
     1981 tctagcgtcg agtgtggtaa agggagaagc ggcgtccacg ataaagagcc gcttcttgca
     2041 ggagtctgga aggagaaagt cgctaccagc gattgccgcg atcgccccgg tcgccgcgat
     2101 ctccacggtc gccgcggccg cgcccgccgc caccaccgcc gccatgtcca ccacggcctc
     2161 cgccgccacc gccgccgaaa ccgcgcccgc caccaccgcc gccgcgttgc tcctggggac
     2221 gagcttcgtt gacgaccaga ttgcgcccgc caaagttctg gccattcatg gaattgaccg
     2281 cgcgatccgc ttcttcgtcg ttggcaattt caacgaaagc gaaaccacgc gattggcccg
     2341 tgaaccggtc acgaatgaga ttgactgccg agggattaac accagcctgc gagaaaaaat
     2401 catgcagcga atcttccgtt gcggaaaacg gaagattacc aacgtagagc ttcttcactt
     2461 acggactcct tctgcccgga acctaactgc gtatcgcgga gcgcgctgga aggccgtgac
     2521 gatccttacg ggcagtccgg agaaaccgaa gccaccttga ttgcaaggcg attggcgcgg
     2581 gccttgaacc tggggtaccg aatcgggcgc tccagcggtc agaggcatcg tccaagaaat
     2641 tgacaagttt tgcaatgaga aattgctgaa tccgggctag cggccactcc ccgcgccggg
     2701 taaatacagt acatcggaat aagtgatagg atgccgtcaa gtaccgcctc cggaggaatt
     2761 cgatgcgcgg caccatgatg gatttcccct tgaccctggt gacgctgctg gagcgggcgg
     2821 gcaagctttt ctccgatgtt gaaatactgt cgcggggccc ggatcgttcg gtggtgcgaa
     2881 cgaattatgg aacgttttat aagcgcgcgc ggcgcctggc cagcgcactg acgaagctgg
     2941 gcctgcaacc gggcgaccgt gtcgcctcca tgatgtggaa tcacgtcggc cacctggatg
     3001 cgttctttgg agtgccctgc gcgggcggaa tcctgcacac gctgaatttg cggctgcatc
     3061 cgcacgagat tgcggcgatt gcgcagcatg cgcgagaccg cttcctgctg atcgacgatg
     3121 tactgctgcc ggtattggaa gaatttcggg cggatgcccc gtttgaaaat gtgattgtcg
     3181 cgccgtacgg ctgcggcggc gcggtgccag acgggttttt gaacctggaa gaactgctgg
     3241 cggaagccgg cgacgatttc aaatatcccg caattgacga aaatgctggc gcggcaatgt
     3301 gcttcacgtc cggcacgacc ggcttttcga aaggcgtgat ttattcgcac cgctcgctgg
     3361 tgctgcattc catggcggag tgcatggtgg atgcgtttgc gctaagcaac aacgatacag
     3421 tgttgccggt ggcgccgatg ttccacgcca acgcctgggg cgtgccgtat acctgcgtga
     3481 tgacgggcgc aaagctgatg ctgagcgggc ctaacgtaga accggaaggc ttgctggact
     3541 ggatggtcga agagcgggcg acgtttgtca gcggcgtgcc aacggtatgg atcggcgtgc
     3601 tggatacgct cgaaaaaaat cccggccgct ggaagtttga ctggcctgtc cgcatcattt
     3661 gtgggggcac agcgcctcct gtggagctga ttcgaaaact ggatcgtttc ggtttgcagc
     3721 tgattcattt gtggggcatg acggaaaccg caccattggc gacaactggg cgactgaaag
     3781 cgaacatgcg cgactggccg gaagaaaaaa aatatgaagt gcgcgcgagg cagggatggc
     3841 cggtgccgtt tttggaattg cgcaccatgc ggccggaagg cgaggctccc tgggatgacg
     3901 aaacgccggg cgagatcgag gtgcgcggcc cgtgggtggc cgcggaatat tttgaatcgc
     3961 cggatcaggc gcatcgctgg accgcggacg gctggtttaa aaccggcgat gtggcgacga
     4021 ttgatcgcga tgggattgtg aagatcgttg atcgcgcgaa agacctggtg aaaagcggcg
     4081 gcgagtggat cagctcgctg gacctggaaa atacgttgat ggggcatccg gcggtgaaag
     4141 aggcttgcgt ggtgggcgta ccgcatccga aatggcagga gcggccgctg gcggcgattg
     4201 tgctaaaaga cggcaaggcg gctagcccgg aggagttgcg cggatttctg gcggcgacgt
     4261 ttgccaagtg gcaactgccg gacgcgtttg tgtttttgga tgcgattccg cgcaccagcg
     4321 tcggcaaatt taagaaaatt gcgttgcgcg aacagttcgc ggattgggag tgggaaagtt
     4381 agtcgtcggc cttgcgccgg ctggtgacat tagccactgg cggacactac gctcgaaccg
     4441 tagcgttttt tccattcggc gcgcatctcg tccgacgccg gccattgcag gcataacctg
     4501 tagccatcgg gatccagtag atagagctgg cgcattccgt acggtgcggt ctgcggttcc
     4561 tgcgttttaa tgcccttcgc ccgcaaatat tcgta
//
LOCUS       DASTPM010000271         3850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1277997, whole genome shotgun sequence.
ACCESSION   DASTPM010000271 DASTPM010000000
VERSION     DASTPM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277997"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFR42_04800"
     CDS             complement(<1..546)
                     /locus_tag="VFR42_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0368501.1"
                     /translation="MVETVNSEEFVLLPQKKKIEDYEFVKVRLDGGVARMTLSRPEHN
                     LLNEAMLRELAEGIEHVGSREDIKIILLDSACKVFCGGIEVGEYTSQRVFQMLDAFHA
                     AFAAMVDVAKPVICVVNGPAIGGGAELAAFGDMVIATPKARFAQPEITIGMFPPLAST
                     ILPYLVGPKVALELVLTGEPVT"
     gene            699..1355
                     /locus_tag="VFR42_04805"
     CDS             699..1355
                     /locus_tag="VFR42_04805"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEU0368502.1"
                     /translation="MSWASAATGTAIALAFSGAPDHEGFPMQEAWAKAQPVQFDADWQ
                     GANSDAQRETEVRLLWTRETLFLRFRCRYRNLTTFPDSDPDGRRYQLWDRDVAEVFLQ
                     PDPANPHRYWELEISPDGKWIDLDINLDKPPGHKENPHSGMKSRVYLDEKQKIWTAEI
                     ALPMRTIKPMFDPKADWRVNFFRVEGPNEPRFYSSWRPTNTPKPSFHVPEAFGKLSFR
                     "
     gene            1386..3317
                     /locus_tag="VFR42_04810"
     CDS             1386..3317
                     /locus_tag="VFR42_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU0368503.1"
                     /translation="MSFLTLRVIATFLVTASVFLGSNSISAAPQAGQASAAPAVTKVE
                     PPNWWVRLTPDLMILLSGHGLQATKVACNLPDVLVLRTEATQGGEYLFVWLKFGPQLR
                     SGTAVCRATTPTGEASFELPIAARLPTAQRFHGLNSDDVIYLIMPDRFSNGDPTNDEP
                     AEFPGSHDRTKPRAWHGGDLKGVRDHISYLKELGVTTVWLTPVVKNGATQDYHGYGAV
                     DLYAVDPHLGSLDDYKGLASDLHKQRMKLFFDAVPNHVGPKHPWVAKPPMADWFHGTA
                     DKHLDSYSPIKNSFYGQGAEAPVKNDPFEALVDSHATPAMRQNLTDGWFLGILPDLNT
                     ENPVVAKYLLQNSIWWIESAGLDGLRVDTFPYVSRTFWQQWHAGLRRLYPNLTTIGEV
                     FHPAPVVTSFFVGGQKGWDGIDTGLSTVFDFPLFFAIRDVLLHSAPVGRIANVLRQDE
                     LYSHPDWLVPFFANHDVPRMISEPGSSPEKLLSALALVLTLRGIPELYYGDEIGVTGG
                     GDPDNRHDFPGGWPGDAQNAFTQAGRTAEQQRILAATQKLLKLRREHAALRTGKLFHL
                     FSDDGAYIFLRQTDDERIVVVFNNGNARRELLIPQSNTPASNAVRTSLLYGDAVATTN
                     GKELRITAPAQSVSIFSLD"
     gene            complement(3332..>3850)
                     /locus_tag="VFR42_04815"
     CDS             complement(3332..>3850)
                     /locus_tag="VFR42_04815"
                     /inference="COORDINATES: protein motif:HMM:NF028447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HEU0368504.1"
                     /translation="KAIQLPPGTWYNYWTGEKTSDRELIKMNPALDEVPVYARAGAIV
                     PRQPLVQNTGETPNGPLELRIYPGSDCKGSLYQDDGHSFNFEKGEFLRVNYACQLAAS
                     GIKIISAVETDGYKPWWNSAQIMVYGFDKAPQSVRVGDTMIRDWHYDSVQQAAVFTLP
                     DAKSNWTAELIF"
BASE COUNT          806 a         1098 c         1124 g          822 t
ORIGIN      
        1 agttaccggc tcaccggtaa gaacgagttc gagcgctact ttcggcccta ctaaataagg
       61 aaggatcgta gaggccaggg gcggaaacat gccgatggta atttccggct gagcaaagcg
      121 cgctttggga gtggcgatga ccatatcgcc gaatgcggct aattccgcgc cgccgccaat
      181 cgctggaccg ttcaccacgc agatgaccgg cttggccacg tccaccatgg ccgcaaaggc
      241 cgcgtgaaac gcgtccagca tctggaaaac ccgctgcgag gtgtattcgc cgacttcgat
      301 gccaccgcag aaaaccttgc aggcggagtc cagcagaatg atcttgatgt cttcgcggga
      361 gccgacgtgt tcaattcctt cggccagttc gcgcagcatg gcttcattga ggaggttgtg
      421 ctcggggcgg ctgagggtca tgcgggccac gccgccgtcc aggcggacct ttacgaattc
      481 gtagtcttca atcttcttct tctgcggcag aagaacgaat tcttcagaat tgaccgtttc
      541 gaccatcttc gcctccgcgc cagcggctga gcgcagctat ccctcagttg gtcgatgcta
      601 gcatggcggt tccaggcggt ttcgtccttg aacaagactg gtgcgcaaag agtttgctgt
      661 cccgatggcc gagggagttc gctatcctat ggagcaccat gagctgggca tcagcggcaa
      721 caggaacggc aatcgcactg gcgttttcgg gtgcgccgga ccacgaagga tttccaatgc
      781 aggaggcctg ggccaaggcg cagccggttc agtttgacgc ggactggcag ggcgcgaatt
      841 ccgatgcgca gcgcgaaacc gaggttcgac tgctttggac gcgcgaaacg ctgttcctgc
      901 gctttcgctg ccgctaccgg aacttgacca ccttcccgga cagcgacccc gacggacggc
      961 gttaccagtt gtgggatcgc gacgtggcgg aagtttttct gcaacccgac cctgccaacc
     1021 cgcaccgcta ttgggaattg gaaatcagcc cagacggcaa atggatcgac ctggacatta
     1081 atctggacaa gccaccgggg cataaggaaa acccgcacag cggaatgaaa tcgcgtgtgt
     1141 atctggacga aaagcagaaa atctggacgg cggaaattgc gctgccgatg cggaccatca
     1201 agcctatgtt tgatccaaaa gcagactggc gcgtgaattt ctttcgcgta gagggaccga
     1261 acgagccgcg cttttattcc tcgtggcgcc cgacgaacac accgaagccg agttttcacg
     1321 taccggaagc attcggcaaa ctgagcttcc ggtaaaaggc gcgccagacg tattcttacc
     1381 tcgccatgtc ctttctcacg ttgcgcgtaa tcgcgacttt cctggttact gcctcggtat
     1441 tcttgggatc gaattcgatt tcggctgcgc cacaggcggg ccaagccagt gcagcacccg
     1501 ctgtaaccaa agtggaaccg ccgaactggt gggtgcgact aacgccggat ttgatgattc
     1561 tactgtccgg tcacggatta caagccacca aagtcgcctg caatttgccg gacgtgctgg
     1621 ttttgcgcac ggaagctaca cagggcggcg aatacttgtt cgtgtggctg aagttcggcc
     1681 cgcagttgcg ctccggcacc gcggtgtgcc gcgcgacgac accaaccggc gaagcatcct
     1741 tcgaattgcc gatcgccgcg agactgccga ccgctcagcg ctttcatggc ttgaattcgg
     1801 atgacgtgat ctacctgatc atgccggacc gtttttccaa cggggatcct accaacgacg
     1861 agcccgcgga atttcccggt tcgcacgatc gcacaaagcc gcgggcctgg cacggcggcg
     1921 atctgaaagg cgtccgcgat catatttctt atttgaagga gctgggagtc accacggtgt
     1981 ggctgacgcc ggtggtgaaa aacggagcga cgcaggacta ccacggctac ggcgcagttg
     2041 atctttacgc ggtcgatccg cacctgggtt cccttgacga ctacaaaggc ctggcgtccg
     2101 atctgcacaa gcagcgcatg aaactgtttt tcgatgcggt gccgaatcac gtcgggccaa
     2161 agcatccgtg ggtggctaag ccgccgatgg ccgactggtt tcatggcacc gcggacaaac
     2221 atctggattc gtattcacca ataaaaaact ctttttacgg acaaggcgcg gaagcgcctg
     2281 tcaaaaacga tcctttcgaa gcgctggttg actcgcacgc gactcccgcg atgcggcaaa
     2341 acctgacgga cggctggttt ttgggcatcc tgcccgacct gaatacggag aatccggtcg
     2401 ttgcgaaata ccttttgcag aattccattt ggtggatcga atcggcgggc cttgacggat
     2461 tgcgagtaga tacgtttccg tatgtctcac gcacattctg gcaacaatgg cacgccggcc
     2521 ttcgccgcct gtatccgaat ctgacgacca tcggcgaagt atttcatcca gctccggtgg
     2581 tcacgtcctt tttcgtgggc gggcaaaagg gttgggatgg aatcgatacc ggcctttcga
     2641 cggtttttga ttttccgttg ttctttgcga tccgcgatgt gttgctgcac agcgcgcctg
     2701 ttggccgcat cgcgaacgtg ctgcggcagg atgagctgta ttcgcatccg gattggctgg
     2761 tgccgttttt cgccaaccac gatgtgccgc gcatgatcag cgagccgggc agttcgccgg
     2821 agaagctgct ttcagcactt gcgctggttc tcacgctgcg tgggattccc gaactttatt
     2881 acggcgacga aatcggcgtg accggtggcg gggacccgga taaccggcac gattttccgg
     2941 gcggctggcc cggtgacgcg cagaatgcat ttactcaggc gggccgcacg gcagagcagc
     3001 agcgaatctt ggcagcgacg cagaaactgc tgaagcttcg ccgcgagcac gcggcgttac
     3061 gaaccggaaa gctctttcac ctgttttctg acgatggagc gtacattttt ctgcgtcaga
     3121 ccgacgatga gcgaattgtt gtagtattca acaacggtaa cgcgaggcgg gaactgctga
     3181 ttccgcaatc gaacacgccc gcgagcaatg ccgtgcgaac gagcctgctt tacggcgacg
     3241 cagtggctac gactaacggc aaagaactcc gcatcaccgc gccggcccaa tccgtctcca
     3301 ttttttccct ggactaagct gttacggaag tctagaaaat cagttccgct gtccaattgc
     3361 tctttgcgtc gggaagagta aagacggctg cctgctgaac cgaatcgtaa tgccagtcgc
     3421 gaatcatggt gtcgccgacg cgaacgctct gcggcgcctt atcaaatcca tagaccatga
     3481 tttgcgccga attccaccac ggcttatagc cgtccgtttc aacggcgctg ataattttaa
     3541 tgcccgaggc cgccagctga cacgcatagt tcacgcgcag gaattcgccc ttttcaaaat
     3601 taaaggaatg gccgtcatcc tgatagagcg aacctttgca gtcggagccg ggatagatgc
     3661 gtagttccag cgggccgttt ggcgtctcac cggtgttctg caccaatggc tggcgcggta
     3721 cgattgcgcc ggcacgggcg taaaccggaa cttcatcgag agcaggattc atcttgatca
     3781 gctcgcgatc ggacgttttc tcgccggtcc agtagttgta ccaggtgccc ggcggcaact
     3841 ggatcgcctt
//
LOCUS       DASTPM010000272         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_182489, whole genome shotgun sequence.
ACCESSION   DASTPM010000272 DASTPM010000000
VERSION     DASTPM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182489"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFR42_04820"
     CDS             complement(<1..190)
                     /locus_tag="VFR42_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0368505.1"
                     /translation="MAIQKVGVVGCGLMGSGIAQVCAQAGFPTIVREVSAEIVEKGLK
                     SIDKNLARLVEKNTITDAQ"
     gene            complement(293..1483)
                     /locus_tag="VFR42_04825"
     CDS             complement(293..1483)
                     /locus_tag="VFR42_04825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEU0368506.1"
                     /translation="MFPTDVVIVAGARTPMARYTGAFSDVSAIDLAAHASKEAIRRSG
                     ADAGEIDHAIFGNVMQTSADALYGARHVGLKAGLKTETPAVTVNRLCGSGIEAIAQGA
                     QRLLLGEAKMVLAGGMENMTQAPFVIRGARGGLKLGGGALEDSLMAGLTDSYCGLPMA
                     LTAEKLAEQQGITRKDADAYALRSQQAADAAYKACRIREELVAVEVKQGKKTITVSED
                     DHRRPETTMEILEKLQPSFKKDGIVTAGNASGIVDGAAAVVVTREKTAKEQRLKPVGR
                     IISWAVAGVDPSIMGIGPVPSSQKALQLAGLTLEQMDRVEVNEAFAAQYLAVEKVLGL
                     NRDKTNVNGGAIALGHPLGASGTRLVITILNELRRNNLRYGLTTACIGGGQGIAMIVE
                     ALYH"
     gene            complement(1510..>2798)
                     /locus_tag="VFR42_04830"
     CDS             complement(1510..>2798)
                     /locus_tag="VFR42_04830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0368507.1"
                     /translation="IVQTEIFLQYSLQNKALERLQKIAATFPGEEERNTRLRNLYQTA
                     NWWPSGAAPSSASGAGAPKTVSASPARPAEPVPESPGGITQKTGVYTAETLRDLAKIS
                     EINQKIFRQQTPRAMLNTAVNEVGAYLKCSRALAVFGASGRPPEMAAEFCAAGMRATP
                     GSQVVQLLAQMEKVQPDELGGLTLNASGSTILKDMGMSAALGVSLTDKETQSPAGMLI
                     VAHAEPYKWKPNEAYFLQAIGDQMLMSVSHTRLRSLVRRMGVSDERTGLLSRSSYQSC
                     LLTEADRSRTQGTPLSLAILQIDRGAELLRQQGESPVEGFLEQLAKSLQPIVRQNDVA
                     VKYTSWSLAFILPDTALAGAQNLATKLRRAASGLRPPWDSGQLTLSAGIVEAVAKSDY
                     ESEDIVTDLINRAETSVEEARKRGGDTIVALESPKL"
BASE COUNT          518 a          912 c          782 g          586 t
ORIGIN      
        1 tctgcgcgtc ggtaatggtg ttcttctcga ccaacctcgc aagattcttg tcgatgctct
       61 tcagtccctt ctcgacaatt tccgcactga cctcccgcac gatggtggga aagccggctt
      121 gcgcgcaaac ctgcgcgatt ccggagccca tcaagccaca ccctacgacg ccaacttttt
      181 gaatcgccat tcggttcctc tgtaggaaat gccgcggtac gtcgccgaag gagcggcaac
      241 ccgcaggaat gcctggtttc ggcaggcaaa agtgtagcag aaacgccgcg aactagtggt
      301 acaaggcctc gacgatcatg gcgatgccct ggccgccgcc gatgcacgcg gtggtcagtc
      361 cgtaacgcaa gttgttgcgg cgcagttcgt tcagaatggt gatgaccagg cgcgttccgg
      421 aggcgccgag cggatggccg agggcaatag cgccgccatt cacattggtc ttgtcgcgat
      481 tcaaacccaa aactttttcc accgccagat attgggcggc gaacgcttcg ttgacttcca
      541 cgcgatccat ctgctccagg gtaagcccgg ccagctgcaa tgctttctgc gatgaaggca
      601 ctggcccgat gcccatgatg ctggggtcga cgccggcgac ggcccaggaa atgatgcgcc
      661 cgaccggctt cagcctctgc tccttcgccg tcttttcccg cgttacgacc accgcggcgg
      721 ccccatccac gattccgctg gcattgcccg cagtgacgat gccgtctttc ttgaacgaag
      781 gctgcaattt ttccagaatc tccatggtcg tttcgggccg ccgatgatcg tcttcgctga
      841 ccgtgatggt cttcttaccc tgcttcactt ccaccgccac caattcttca cgaatgcggc
      901 aagccttgta ggcagcatcc gccgcttgct ggctgcgcag tgcgtacgcg tccgcgtctt
      961 tgcgggtaat tccctgctgc tcggcgagtt tttcagcggt cagggccatt ggcaatccgc
     1021 aataagaatc tgtcagcccc gccatcagcg aatcctccag cgccccgccg ccaagtttca
     1081 gcccgccgcg agcaccacga atcacaaacg gcgcttgcgt catattttcc atgccgcccg
     1141 caagtaccat cttggcttca cccagcaaca agcgctgcgc gccctgtgcg atggcctcaa
     1201 tcccagagcc gcacaaccga ttgacggtaa ccgcgggtgt ttccgtcttc agtcccgctt
     1261 tcaaccccac gtgccgtgct ccatacagcg cgtcggcact ggtctgcatg acattgccaa
     1321 aaatcgcgtg atcgatttcc ccggcatcgg cgcccgagcg gcggatcgct tccttggaag
     1381 catgcgccgc cagatcaatc gcggagacat ctgaaaacgc gccggtataa cgcgccatgg
     1441 gcgtgcgtgc tccggcaaca atcactacat cggtcgggaa catatttttc ctctctaatt
     1501 tgttgagggc tagagtttgg ggctttccag ggccacgatg gtgtcgccgc cccgcttacg
     1561 cgcttcttcc acgctggttt ccgcgcgatt gatcaggtcc gtaacaatgt cctcactctc
     1621 ataatccgat tttgccaccg cttcgacaat tcccgcgctc agcgtgagct ggccggaatc
     1681 ccacggtgga cgcagaccag atgcggcccg gcgcagcttg gtggccagat tctgcgcgcc
     1741 agccagcgcg gtatccggca aaatgaaggc cagcgaccac gaggtatatt ttaccgcgac
     1801 atcgttctgc cgcacgatag gctgcagcga cttggccagt tgttcgagaa atccttccac
     1861 cggcgattca ccctgttgcc gcaagagctc tgcgccgcgg tcaatctgca gaatggccag
     1921 cgacagcggc gtgccctggg tgcgcgagcg atccgcctcg gtcagcaaac aactctggta
     1981 ggagctgcgg ctcaacaagc cggtgcgttc atcggaaacg cccatgcggc gcaccagcga
     2041 acgcaggcgc gtgtggctga cgctcatcaa catctggtcg ccaatggcct gcaggaagta
     2101 cgcctcgtta ggtttccatt tgtagggctc ggcgtgcgcg acaatcagca tgccagccgg
     2161 gctctgcgtt tccttgtcgg tcaggctgac tcccagcgcg gcgctcatgc ccatgtcttt
     2221 cagaatcgtg ctgcccgaag cattcagggt caacccgccc agttcatcgg gttgcacttt
     2281 ctccatctgc gccagcaact gcaccacttg cgagccgggc gtggcgcgca tgcccgccgc
     2341 gcaaaactcc gcggccatct ccgggggccg ccccgaggcg ccaaaaacgg ccagcgcacg
     2401 cgagcacttc aaatacgcgc cgacttcgtt gaccgcggtg ttgagcatgg ctcgcggggt
     2461 ctgttggcgg aaaatcttct gattgatttc ggaaatcttg gccaagtcgc gcagcgtttc
     2521 cgccgtgtac acccccgttt tttgcgtaat gccaccgggg gattctggca ccggctcggc
     2581 tggccgcgcc ggcgacgcag atacggtttt cggcgcacca gcgccggaag cagagctggg
     2641 agcggcacca gacggccacc aattcgcggt ctgatataaa ttgcgcagcc gcgtattgcg
     2701 ctcttcttca cccgggaagg tggcagcgat tttctgcaac cgctccagcg ccttattctg
     2761 cagcgaatac tgcaggaaaa tttccgtctg cacgatca
//
LOCUS       DASTPM010000273         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_931962, whole genome shotgun sequence.
ACCESSION   DASTPM010000273 DASTPM010000000
VERSION     DASTPM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931962"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFR42_04835"
     CDS             <1..529
                     /locus_tag="VFR42_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU0368508.1"
                     /translation="TFQNGAFRGDKVEAGTSAAPVETIAVDIGDGVVRNKPQEVFKEA
                     KQAVDLTQAEVIVAVGRGIKEQKNIEIAKQLAEALGGELAASRPICDSGWLPMDRQIG
                     SSGQTVAPKLYLALGISGAIQHIVGMKGARTIVAVNKDSEAPIFEIADYAVVGNLFEV
                     VPPLIEEIKKAKAGA"
     gene            590..2305
                     /locus_tag="VFR42_04840"
     CDS             590..2305
                     /locus_tag="VFR42_04840"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEU0368509.1"
                     /translation="MLAFRTPLENIDRPQMEADVVIVGGGPAGMACALRLSQLIDEHN
                     AKHPDALLSKENIYVLEKAREVGQHCLSGALLDPRSMRELLPGFEKEAPIDAQCSQEA
                     VYFLTKNGKFKLPITPPPLRDHGNYVISINKFVKWLGSKVEETGITIFTGFAGSQLLY
                     DGNRVAGVRTDDKGVDKENHPKSNFEPGYDLKAKITILAEGTRGSLTKELINKFDLAK
                     DRNPQTYGVGVKELWEVPSDRIAPGEVIYTMGWPLTTKEYGGAWIYGAKDNLVSLGFV
                     IGLDYPDPRLDPQHVLQDFKQHPFVAKLLAGGKMIRYGAKSMPYGGWWAVPPVAGNGW
                     MILGDSAGLVNSQRLKGIHLAIKSGMLAAETAFEALKKGDASAATLAQYQQKVDASWI
                     KEELWKVRNFHQGFEKGFFAGMFHAGLQQFTGGRGLWNRYPAHAGHERLEKLSQLPAD
                     GGNEAHLLGSAKGDGKLTFDKLTDLYHSGTKHEEDQPSHLVIADTNVCNARCAVEYSG
                     PCQNFCPANVYELVDDANQPHGKRISLNPANCVHCKTCDIADPYQVINWVPPEGGGGP
                     NYDGM"
BASE COUNT          549 a          699 c          677 g          429 t
ORIGIN      
        1 gaccttccag aacggagcgt tccgcggaga caaagtggag gccggtacca gtgcggcgcc
       61 ggttgagacc atagccgtcg acatcggcga tggtgtcgtc cgtaacaagc cgcaggaggt
      121 ctttaaagaa gccaagcaag cggtcgacct aacccaggca gaagtcatcg tagccgtggg
      181 ccgaggaatc aaagaacaga agaacatcga aatcgcgaag cagttggccg aagcgttggg
      241 cggcgaactc gcggcgtcgc gtcctatctg tgattccggc tggctgccca tggatcgcca
      301 gatcggctct tctggtcaaa ccgtcgcgcc gaaactttat ctggcgctgg gaatctccgg
      361 ggccatccaa cacattgtcg gcatgaaagg cgcgcgcact atcgtggctg taaacaaaga
      421 tagcgaagcg ccgatcttcg aaattgccga ctacgccgtg gtaggcaatc tatttgaagt
      481 cgttccgccg ctgattgaag aaatcaaaaa ggccaaagcc ggagcctaat gcggcctgcg
      541 tttaacgcat gcaaatggtt gtggcgagac gctaaaggct aattgctaaa tgcttgcttt
      601 tcgcactccc ctcgaaaata tcgaccgccc ccagatggaa gccgacgtgg tcatcgtggg
      661 cggcggcccg gcgggtatgg cctgcgcttt gcggctttcg cagctcatcg acgagcacaa
      721 cgccaagcac cccgacgccc tgctcagcaa agaaaacatc tacgttctgg agaaggctcg
      781 cgaagttggc cagcactgtc tttccggtgc gctgctcgac ccgcgctcca tgcgcgaact
      841 ccttccgggt ttcgagaaag aagcgcctat cgacgcacag tgcagccagg aagcagtcta
      901 cttcttaacg aagaatggca aattcaaact gccgatcacg ccgccgcccc tgcgcgatca
      961 tggcaactac gtcatctcga tcaacaagtt cgtgaagtgg ctgggttcca aggtcgaaga
     1021 aaccggaatc acgatcttca ccggtttcgc cggatcgcaa cttctctacg acggcaatcg
     1081 ggtcgcaggt gttcgaaccg acgacaaggg cgtcgataaa gaaaatcacc ccaagtccaa
     1141 tttcgaaccc ggctacgacc taaaagcgaa aatcacaatc ctcgccgaag gcacgcgcgg
     1201 ctcactgacc aaagagctaa tcaacaagtt cgacctcgcc aaagatcgca acccgcaaac
     1261 ttatggcgtc ggcgtcaagg aactctggga agtgccctcg gaccgcattg cgccgggcga
     1321 agtgatttac acgatgggct ggcctctcac cacgaaggaa tacggcgggg cttggatcta
     1381 cggcgccaag gacaatctcg tctcgcttgg ttttgtcatt ggtctcgatt atcccgaccc
     1441 acgccttgat ccgcagcatg tgctgcagga cttcaagcaa catcctttcg tcgcgaagct
     1501 gcttgcgggc ggcaagatga tccggtacgg ggcgaagtcc atgccctacg gcggatggtg
     1561 ggcggtcccg ccggttgcgg gcaacggctg gatgatcctc ggcgattcgg ccggcctggt
     1621 caactcgcag cgcctgaaag gcattcatct cgccatcaag agcggcatgc tcgcggccga
     1681 aaccgcattc gaggcgctga agaagggcga cgcctccgcc gccacgctcg cgcagtatca
     1741 gcagaaagtc gacgcgagct ggatcaaaga agaactctgg aaggtgcgaa acttccacca
     1801 gggatttgaa aagggttttt tcgcagggat gttccacgcg ggcctgcagc aattcactgg
     1861 cggccgcggc ctgtggaacc gctatccggc ccacgctggg cacgagcgtt tggaaaagct
     1921 ttcgcaactg cccgccgacg gaggtaatga agctcacctg ctcggctccg ccaaaggcga
     1981 cggcaagctt accttcgaca agctcaccga cctctaccac tccggcacca agcacgaaga
     2041 agaccagccc tcgcacctgg tgatcgccga caccaacgtc tgcaacgcgc gatgtgcggt
     2101 ggagtatagc ggtccttgcc agaatttctg tcccgcgaac gtgtatgagt tagtggatga
     2161 cgcaaaccag ccgcatggta agcgcatcag cctgaacccg gcaaactgcg tgcattgcaa
     2221 aacttgcgac atcgcggatc cctaccaggt catcaactgg gttccacccg aaggtggggg
     2281 cgggccgaat tacgacggaa tgtgaaccag tgtgggcgca gtctccctca cctgcgatga
     2341 gggcaaggcc tcac
//
LOCUS       DASTPM010000274         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_932000, whole genome shotgun sequence.
ACCESSION   DASTPM010000274 DASTPM010000000
VERSION     DASTPM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932000"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2354)
                     /locus_tag="VFR42_04845"
     CDS             complement(<1..2354)
                     /locus_tag="VFR42_04845"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEU0368510.1"
                     /translation="MTRQPANWPSISGNSAVWALLAYLTLAAAPAFSQSGRPGKLRCE
                     SLASPLGVDARQPVFSWQLRDTRYGAKQSAYQLEVASSEKLLASGKSDIWDSGKIQSD
                     HSIGVAFAGRPLKPSTRYFWRVKVWGRDGKPYPASETAWWETGLMEQQNWRAKWIGYD
                     EPELAAVRKANAEWITNSETDSAATTGDTHHEFRFHFDASKKVQRALLYVTGQDSAAA
                     WVNGQQVLESQPLPPWKQMPWKTYSVKDITKNVARGPNLLGVEVVRYREERRRSRPDD
                     FRAPMSATIYLQFADGSTQIFKSSAQGWRAALDAKGDWRDANFADSAWTEAIRYTPPA
                     ESFDGPLGDPWPTGAVKLLRRTFDVAKPVAAARLYVTALGAYKLSINGKEVGDQILAP
                     GWTDFRERVIYQTYDVTGNLKSGRNAIGALLAPGWYATPLQWYRQGNNYGKTPPALRA
                     QLRIAYADGSVDWVATDEKWKADVSPIPSAEIYDGETYDARKAQAGWSTAAFDDGKWK
                     AAEVIQPGETEIVWQYFPPIREHQVLSAKSITNPGAGVYVFDFAQNLSGVARVRATGV
                     EGTEVKLRFAEVLNDDGTLYVENLRTAKATDHFILSGRRAEEFEPKFTFHGFRYVEVS
                     GLSSKPELGDVKAVVFHTDAPFTTELKTGNTMINQLWSNILWGQRSNFVGVPTDCPQR
                     DERLGWTADAQVFWRTATYNMDLATFSRKFSTDMRGTQVGTAMYGIFAPGTSTSNPGY
                     GTGWSDAGVIIPWTSWIQTGDQKVIEENWGGMEQYLAAIESANP"
     gene            complement(2537..3754)
                     /locus_tag="VFR42_04850"
     CDS             complement(2537..3754)
                     /locus_tag="VFR42_04850"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU0368511.1"
                     /translation="MDTITHGIAGALIGKAFCGGDSMFPVKPVSRARIATWALIVGAI
                     FPDSDVFRDMLSRNPLLIITWHRSYTHSLLMLPLFALALAGITRWIARKFGWDAPSYA
                     ALTGIYAIGIASHIFLDLVTTFGTMVWSPLAWSRPAWDLIFIVDFSFSGILLIPQLLA
                     WVHRDEKHAKRRAVMMWMLFVPVTFGIAALGRIVGAPISTATIWSAIGIFSLLFLVPA
                     FFGWGSRLRLATWNRAGFVGACIYVGLTVVAHHQALERVKKFAEFQGVRSDAIAALPF
                     PPSLWNWDGLIRTPRGVYEMRMDLSQPSGIPRTEAEAAAAAKVPLTYNYFPDAPVNNW
                     IQLARQLPEVQKVLWFDRFPVTRFHKEGEDAVVEILDLRFAKVRPDRPASFTYRVRFD
                     ATGKVTEQGWVRE"
     gene            complement(3778..4857)
                     /gene="selD"
                     /locus_tag="VFR42_04855"
     CDS             complement(3778..4857)
                     /gene="selD"
                     /locus_tag="VFR42_04855"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4810..4812),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU0368512.1"
                     /translation="MGDRVKLTSMAKAAGUAAKLSPGTLDSVLRRLPRQTDPNVLVGF
                     ETSDDAGVYLLDETTALVQTVDFFTPIVDDPRTFGEIAAANSVSDIYAMGGRPISALS
                     LVGFPEKGDPEILGEILQGGLSKMMEANCSVIGGHSVRNEDVLFGYSVTGVIHPKRVW
                     KNVGACTGDALLFTKPLGTGVISTALKKEKASAESVNAAIASMRTLNRAAADALHEIE
                     TQEEWSDPIHAVTDVTGFGFLGHAREMALGNPDGGIAPVSMEIDHAAFAYLPGALEAA
                     KDGHIPGGLINNREFVGECAEFAAGVAQEFRDLLFDPQTSGGLLVAISADAAEEALRA
                     MERHGFAGLQVGNAVSRRAPLLFVK"
     gene            4977..5729
                     /locus_tag="VFR42_04860"
     CDS             4977..5729
                     /locus_tag="VFR42_04860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0368513.1"
                     /translation="MLSSPMPWDFWLIFLIVGVVIPWRGHARMRQLMALPEVTSRDRV
                     KMYLATILFQWLLTAFVSWRALARGLSSRELGVSSAASFPLLAVTIAGATLIAAAHWA
                     NVRRMARSDHPNVERLRALGLRLFPRSNSELSLFIVLSLTAGICEEFLFRGFVMAALF
                     RAGLAAWLVVILSSAMFGVAHLYQGKSGSLGTGILGTLFALVRLAYRSLLPAVVWHAV
                     LDIVAGIAGARFLLGPTNTTSGEPPVLDEIHR"
     gene            5790..>6049
                     /locus_tag="VFR42_04865"
     CDS             5790..>6049
                     /locus_tag="VFR42_04865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEU0368514.1"
                     /translation="MSEFGLQLDQHQVQQIQQYTKILLAWNDKVNLTAIRDPLEILYR
                     HFCESMFGAAVLPVEKCRLADVGSGGGFPGLPLKIVRPDLEV"
BASE COUNT         1196 a         1923 c         1608 g         1322 t
ORIGIN      
        1 tccgggtttg cagactcgat tgctgccaga tactgttcca tgccgcccca gttttcctcg
       61 atcacctttt gatccccggt ttggatccag gaagtccatg gaatgatcac gccggcatcg
      121 ctccaaccag tgccgtagcc gggattcgac gtcgacgtgc ccggcgcaaa aattccgtac
      181 atcgcagtgc ccacctgggt gccgcgcatg tccgtactga actttctcga aaacgtcgcc
      241 aggtccatgt tgtacgtcgc ggtgcgccag aatacttgag cgtccgccgt ccagcccagc
      301 cgttcgtcgc gctgcggaca atcagtaggc acgccgacaa aattcgagcg ctgcccccac
      361 aaaatattgc tccacagctg gttgatcatg gtgttgccgg tcttgagttc ggtagtaaac
      421 ggcgcatcgg tgtgaaaaac taccgctttc acgtccccca gttcaggctt gctggaaagt
      481 cccgacactt ccacgtagcg aaagccgtgg aacgtaaatt tcggctcgaa ctcctccgct
      541 ctcctgcccg acagaatgaa atgatccgta gccttggcgg tccgcaaatt ctccacgtaa
      601 agcgtgccgt cgtcgttcag cacttcggca aaacgcagtt tcacctccgt tccctccacg
      661 ccggtggcgc gtacgcgcgc cacgcccgaa agattttgcg caaaatcaaa aacataaact
      721 cccgcgcctg gattcgtgat gctcttggct gacaacacct ggtgttcgcg aatcggtgga
      781 aagtattgcc agacaatctc cgtttcgccc ggctggatga cttccgccgc tttccatttt
      841 ccatcgtcaa aggccgccgt gctccatccc gcctgcgctt tgcgcgcgtc gtaagtttcg
      901 ccgtcgtaaa tttccgcgct cggaatcggc gaaacatccg ctttccactt ttcatccgtc
      961 gcaacccagt ccactgaacc gtccgcatac gcaatgcgca gttgcgcgcg cagcgccggc
     1021 ggtgtctttc cgtaattgtt cccctgccgg taccactgca gcggagttgc gtaccacccc
     1081 ggcgccagca gcgcgccgat cgcgttgcgt cccgatttca ggttgccggt gacatcgtag
     1141 gtctggtaaa tcacgcgctc gcgaaaatcc gtccatcccg gcgcaagaat ttggtcgcca
     1201 acttcttttc cgttgatcga cagtttgtac gcgccgagcg ccgtcacata caggcgggcc
     1261 gcggccaccg gcttcgccac gtcgaaagtt ctacggagca atttcaccgc gcccgtcggc
     1321 cacggatctc ccaacggccc gtcaaacgac tccgccggcg gtgtgtagcg aatcgcctct
     1381 gtccatgcac tgtcggcgaa gtttgcgtcg cgccagtcgc ctttggcatc caacgccgcg
     1441 cgccatccct gcgcgctgct cttgaagatt tgtgtcgaac catcggcaaa ctgcaaataa
     1501 atagtagcgc tcatcggcgc ccggaaatca tccggcctgc ttctccggcg ttcctcgcga
     1561 tagcgcacca cctccacgcc cagcaggttc ggtccgcgcg ccacgttttt tgtgatgtcc
     1621 tttacgctgt acgtcttcca cggcatctgc ttccacggag gcagcggctg cgactccagc
     1681 acttgctggc cattgaccca cgccgccgcg gaatcctgcc cggtgacgta aagcaaggct
     1741 cgctgcactt ttttgctcgc atcaaaatgg aagcggaatt cgtgatgagt atctcctgtg
     1801 gttgccgcgc tatccgtctc cgaattggta atccattccg cattcgcttt gcgaaccgcg
     1861 gcgagttccg gctcgtcata gccaatccat tttgctctcc agttctgctg ctccatcaga
     1921 ccagtttccc accacgccgt ttcgcttgcc ggatacggct tcccatcgcg accccacact
     1981 ttcacgcgcc agaaataccg cgtactcggc ttgagcggtc tgccggcgaa ggcgacgcca
     2041 atggaatggt ccgactgaat tttgccgctg tcccaaatgt cggacttccc ggaggcgagc
     2101 aacttttccg aagacgcgac ttccagttga tacgcggatt gtttcgcgcc ataacgcgta
     2161 tcgcgcagtt gccaggaaaa aacgggttgc ctggcgtcca cgcccagcgg cgaagccagt
     2221 gattcacagc gaagctttcc cggccggcca ctttgcgaaa aagctggcgc cgcggcgagc
     2281 gtcaagtagg caagcagggc ccataccgca ctgttcccgg aaatgcttgg ccagttcgca
     2341 ggttgccgcg tcaccttgtt ctcctcgggc ggattcgccc tggccaccgg aatcgtggct
     2401 cgtcggctat gtgggtcgca cgtagcaagc gtcgggcact ctagcggcaa attcccgacg
     2461 ctgtcaacag aatgtggaaa agtttctgta ttgaaatcta ggctgcgccg cgaagctccc
     2521 ggacgcaacc agtcagctat tcccggaccc agccctgctc cgtaaccttt ccagtcgcat
     2581 cgaaccgcac acgataggtg aacgacgccg gccggtcggg ccgcaccttc gcaaaacgaa
     2641 gatcaagaat ttccaccacc gcgtcctcgc cctccttgtg aaaccgggtc accgggaacc
     2701 ggtcgaacca caacactttc tgcacttccg gcagttgccg cgcaagctgg atccagttgt
     2761 ttacgggcgc gtcgggaaaa tagttgtaag tgagcggcac cttggccgcg gccgccgcct
     2821 cggcttccgt cctcggtatc cccgagggct ggctcagatc catgcgcatc tcatagactc
     2881 cgcgaggagt acggatcaag ccatcccagt tccacagcga tgggggaaac ggcagagctg
     2941 cgatcgcgtc agagcgcacg ccttgaaact cagcaaactt tttcacccgc tctaaagctt
     3001 ggtgatgcgc gacgaccgtc agcccgacat agatgcaggc gcccacgaaa cctgcgcggt
     3061 tccaggttgc cagacgcaac cgcgagcccc atccaaaaaa agcgggcacc aggaataata
     3121 gggaaaaaat tccaatcgcg ctccagattg tcgcggtaga aatcggcgcg ccgacgattc
     3181 tcccgagggc ggcgattcca aatgtcaccg gcacaaatag catccacatc attacggcac
     3241 gccgcttggc gtgtttttcg tcgcgatgca cccacgcaag cagttgggga atcagcagaa
     3301 tgccggagaa cgaaaaatca acgatgaaaa ttaaatccca tgccgggcgc gaccacgcca
     3361 gcggcgacca caccatcgtg ccgaatgtgg tcaccagatc caaaaaaatg tggctggcaa
     3421 tgccgatcgc atagattccc gtcaacgccg catacgacgg cgcgtcccag ccgaatttcc
     3481 tggcaatcca gcgcgtgatg cccgccagcg ccagcgcaaa cagcggcagc atcagcaacg
     3541 aatgcgtgta gctgcgatgc cacgtaatga tcagcagggg gttccgggag agcatgtcgc
     3601 gaaacacatc gctgtccgga aaaatggcgc ccactatcag cgcccacgtc gcgattcgag
     3661 cgcggctcac cggctttacc ggaaacatgc tgtcgccgcc gcaaaacgcc ttgccgatca
     3721 gcgctcctgc aattccatgt gtgatcgtgt ccattcgttt tcgatcttcc ggattattta
     3781 ttttacgaag agcagtggtg cgcgcctgga gaccgcattg ccaacctgga ggccagcaaa
     3841 tccatgccgt tccatcgcgc gcaacgcttc ctccgcggca tcagcactga tcgccaccag
     3901 caatccacct gacgtctgcg gatcaaacag caagtcgcga aattcttgtg ctacgccggc
     3961 ggcgaactcc gcgcactccc ctacaaattc gcggttgttg atcaagccgc ccggaatatg
     4021 gccgtcttta gcggcttcta gcgctcccgg caaatacgca aacgctgcgt gatcgatctc
     4081 catcgagacc ggtgcgattc ctccatccgg attgcccaga gccatttccc gcgcgtgtcc
     4141 cagaaatcca aaccccgtca catccgtcac cgcatggatt gggtcgctcc actcttcctg
     4201 cgtttcgatt tcgtgcagcg catcggcagc ggctcgattc aacgtgcgca tcgacgcgat
     4261 ggcggcattt actgactccg cgctggcctt ttcttttttt aacgcggtcg aaatcacgcc
     4321 agtgcccagg ggtttggtaa acagcaacgc atcgccggtg cacgcgccga catttttcca
     4381 aactcgcttc ggatgaatca ctccggttac cgaatacccg aacagcacat cttcattgcg
     4441 tacggagtgg ccgccgatca cgctgcaatt cgcttccatc atttttgaca gcccgccctg
     4501 cagaatctcg cccagtattt ccgggtcacc tttttcggga aatcccacca atgaaagcgc
     4561 ggaaattggc cgcccgccca tggcgtaaat gtcgctcacc gaattggccg cagcgatttc
     4621 tccaaaggtt cgcggatcgt ccacaatcgg cgtaaaaaaa tcgacggtct gaaccagcgc
     4681 cgttgtttcg tcgagcagat acaccccggc atcgtcgctg gtttcaaatc caacgagcac
     4741 gttcgggtca gtttgccgcg gcagccgccg caacaccgag tccagcgtcc cgggactcag
     4801 cttggctgct caaccggcgg ccttggccat cgacgtcaac ttgacgcgat cgcccatgag
     4861 ggcctccttt gtaactgcgc tggtttctct actttcgcaa atgcacacgc attatagaag
     4921 atgcggtccc gttcgcgctg cctgccgcag cgaaagttgc ggaaacgctc ttcgccgtgc
     4981 tttcatcacc catgccgtgg gacttctggt tgatcttttt gatcgtggga gtcgtgattc
     5041 cttggcgcgg gcacgcgcgt atgcgccagc tcatggcgct tccggaagtt accagccgcg
     5101 atcgcgtcaa aatgtacttg gccacgatcc tttttcagtg gctgttgacg gctttcgttt
     5161 catggcgcgc attggcgcgg ggcttgtctt cgcgtgaact cggagtcagc agcgccgcga
     5221 gctttccgtt gctcgccgtg acaattgctg gcgcgacatt gattgcggcc gcgcactggg
     5281 ccaacgttcg gcgcatggcg cgttcagatc atccaaatgt ggagcgcctg cgggctctcg
     5341 gcttgcgtct tttcccgcga tcaaattccg aactgtccct attcatcgtt ctttcgctga
     5401 ccgccgggat ctgtgaagaa ttcctgttcc gcggcttcgt gatggcggca ttgttccgcg
     5461 ccggcctcgc ggcttggctg gtcgtcatcc tctcctccgc catgtttggt gtggcgcatc
     5521 tttatcaggg aaagagtggt agcctgggga ccgggattct gggcacattg tttgcattgg
     5581 ttcggctggc gtatcgcagt ctcttaccgg ccgtggtctg gcacgcggtg ctcgatattg
     5641 ttgcgggaat tgccggagca cggtttcttc tcgggcccac aaacaccacg tcgggcgagc
     5701 cgccagtttt agatgaaatc cacagatgat ttactataat gacagctact agcatgacgc
     5761 caccctcccc ggctgccatc gagcgcgcca tgagcgaatt tggcttgcaa ttggatcagc
     5821 atcaagtcca gcaaattcaa caatatacaa agatcctgct ggcctggaat gacaaggtaa
     5881 atctcacggc gattcgcgac ccgctcgaaa tactatatcg tcatttttgc gagtccatgt
     5941 ttggtgcggc agtcttgcct gtggaaaagt gtcggctcgc cgacgtaggt tccggaggcg
     6001 gtttcccagg tctccctctc aagattgtgc gtcctgacct ggaggtctt
//
LOCUS       DASTPM010000275         3488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_9611, whole genome shotgun sequence.
ACCESSION   DASTPM010000275 DASTPM010000000
VERSION     DASTPM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9611"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VFR42_04870"
     CDS             <1..1037
                     /locus_tag="VFR42_04870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0368515.1"
                     /translation="GLECRILSLNDKAELKRLNVTGREIVFTGSERAKGNFLAAALKS
                     ARRAAGKGKKAPKIVVAGHPNLAPVVRAMRLVTPRMKAILCTHGVEVWQPLPRMRRNA
                     LCKADVVLAPSKYTADHVAADQGVDTAKIRVLPWALDPQFEALATKASKTPPPAKFPQ
                     GHVVLTVGRWRADERYKGMDTLITALPRLLPRWPELQLVAVGDGDDRAWLEDLAEETG
                     VDRHVHFLQGLNYEELAACYGHCEMFALPSRGEGFGLVYLEAMACGKPVIGGAHGGAP
                     EVIEDGKTGYLVPHGDAAQLAIAMETLLADRTLREEMGRRGKQRVENEFRFNVFSKSF
                     RKILRELCES"
     gene            1025..2290
                     /locus_tag="VFR42_04875"
     CDS             1025..2290
                     /locus_tag="VFR42_04875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0368516.1"
                     /translation="MRILSVTQSYAPFYEFGGPPVKVEALAKGLAQRGHSVTVLTVDW
                     GYQKRKEADADGVSSRRSRFGWTREANGVEGIYLPTRFRYRALSWNPAVERYCRERLG
                     QFDVVHIFGLYDLLGPAVARTCRKQGIPYVIEPIGMFLPIVRNIALKRLYHKFWGHKL
                     LAGAAAVVATSEQEAEELAKGRGPAGQGGIPREKIVLRRNGVMAPRELPARGTFRAKY
                     GLPADSLVVLFLGRLSVKKSPELLLEAFARLPERIEGNTMRLVFAGPDESGMLARLQS
                     QARKLGVETRVIFAGSVFGQEKWTAYRDADVFVLPSQNENFGNSAAEAAACGTPVVVT
                     ENCGIAPLLAGVAGLVVGHETLKVGQAVQQVLSDEALRERLAQGGRSAAGRLGWDEPV
                     SEMARLYEKLAKPTQASQFRPNKETANQG"
     gene            2304..3152
                     /locus_tag="VFR42_04880"
     CDS             2304..3152
                     /locus_tag="VFR42_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU0368517.1"
                     /translation="MGQKLFRISWWTVNVLLLVSLLALAHSTAWEYSVRRYLSGFSDA
                     IVPAGAIPEERVEAILNWMREGPPRLEGSTPTGLAIRDPEKTLNYRQLLTVCGTATNA
                     FLNLARSSDLQVRRLLLLTPYRNTKHVVAEVQLNGRWVVVDPTYRVILRNGAGEMVTL
                     SELKNPQTFAEVIGRVPGYPAEYNYQNYAHVRVARLPMQGWQLRPLLDRVFPGWEERL
                     DWSLLLERASFFALTLSACATLILLGLRLVMGWYADRKLKIPRFRLRAHLARAGAAFL
                     TSPEIK"
     gene            3157..>3488
                     /locus_tag="VFR42_04885"
     CDS             3157..>3488
                     /locus_tag="VFR42_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368518.1"
                     /translation="MCGICGVAFLNTAKDAESRVRAMAAAMRHRGPDGEGFLINDRRA
                     PGLALAMRRLSIIDLAGGQQPIWNETLDVGVVFNGELYNYRELRERLTRCGHQFTTHS
                     DTEILVHA"
BASE COUNT          729 a          860 c         1249 g          650 t
ORIGIN      
        1 gcggattgga atgccgcatt ctgagcctga atgacaaagc cgagctgaag cggctgaatg
       61 tgaccgggcg ggagatcgtt ttcaccggaa gcgagcgcgc gaagggaaat tttctggcgg
      121 cggcgctgaa gagcgcgcgg cgcgcggcgg gcaagggcaa gaaggcaccc aaaatcgtgg
      181 tcgccggcca tccgaacttg gcgccggtgg tacgggcgat gcggctggtg acgccgcgaa
      241 tgaaagcaat cctctgcaca catggcgtgg aagtgtggca gccactgcca cgcatgcggc
      301 gaaacgctct gtgcaaagcg gatgttgtgt tggctccgag caagtacacg gcggaccatg
      361 tggcggcgga tcagggagtg gatacggcaa agatccgcgt attgccgtgg gcgctcgatc
      421 cacaatttga ggcgctggcg acgaaggcgt caaaaactcc tcccccggca aaatttccgc
      481 aaggacatgt ggtattgacg gtggggcggt ggcgcgccga tgagcgctac aagggaatgg
      541 acacgctgat taccgcgctg ccgagactgc tgccgcgctg gccggagctg caactggtag
      601 ccgtgggcga cggcgacgac cgcgcatggc tggaagattt agcggaagag acgggcgtag
      661 accggcatgt tcactttctg caagggctca attacgaaga gctggccgcg tgctatgggc
      721 actgcgagat gttcgcgctg ccgagccggg gagaaggatt cggactggta tacctggaag
      781 cgatggcgtg cggtaagccc gtgatcggtg gagcgcatgg cggtgcgccg gaagtgattg
      841 aggatggaaa gacgggctac ctggtaccgc acggagacgc ggcgcaactg gccatagcga
      901 tggaaacttt gctggcggat cgaacactgc gggaggaaat ggggcggcgc gggaagcagc
      961 gcgtggaaaa tgaatttcgg ttcaatgtgt tttccaaatc tttccggaaa atcttgcgcg
     1021 agttatgcga atcctgagcg ttacacaatc ctacgcgccg ttttatgaat ttggcgggcc
     1081 accggtgaaa gtggaagcgc tggcgaaggg gctggcacag cgcgggcaca gcgtgacggt
     1141 gctaaccgtg gactggggtt accagaagcg aaaggaagcg gatgcggacg gagtgagttc
     1201 gcgccggtcg cgatttgggt ggacgcggga agcgaacgga gtcgagggca tctatctgcc
     1261 gaccaggttt cgatataggg cactgagctg gaacccggcg gtggaacgat attgccggga
     1321 acggctgggt caattcgatg tggtccatat cttcggcttg tacgatttgc tgggaccggc
     1381 ggtggcgcgc acttgccgca aacaggggat tccctacgtg atcgagccga tcgggatgtt
     1441 cttgccgatt gtgcgcaaca ttgccttgaa gcggctctac cacaaatttt ggggccacaa
     1501 actgctggcc ggcgcggcgg ctgtggtggc aacgtcagag caggaagccg aggaattagc
     1561 caaaggccgt ggccccgccg ggcaaggtgg aatcccacga gagaagatcg tgctgcggcg
     1621 aaatggggtg atggctccac gagagctacc agcgcgaggc acgttccggg cgaagtacgg
     1681 gctgccagcc gactccctgg tggtgctgtt tctgggccgg ctttccgtga agaaaagtcc
     1741 ggaattgctg ctggaggcgt tcgccagact tcccgagcgg atagaaggaa ataccatgcg
     1801 tctggtattc gcaggtccgg acgaatccgg catgctggcg cggttgcaat cccaggcgag
     1861 gaaactgggc gtggagacgc gggtgatttt tgcaggtagc gtcttcggcc aggaaaagtg
     1921 gacggcgtac cgcgatgccg acgtgttcgt gctgccctca cagaacgaga atttcggcaa
     1981 tagcgcggca gaagcggcgg cttgcgggac gccggtggtg gtgacggaga actgcgggat
     2041 cgcgccgttg ttggcgggcg tagcgggact cgtggttggg catgaaacct taaaggtggg
     2101 tcaggccgtc caacaggtat tatctgacga agcgttgcgc gagcgcctgg cccagggcgg
     2161 aagaagcgcg gcggggcgtc tcggttggga cgagccggta agcgaaatgg caagactgta
     2221 cgagaaactc gcgaagccga ctcaggccag tcaatttagg ccaaacaaag aaaccgcaaa
     2281 ccagggctag cagtgtgttg agcgtgggac aaaaactctt tcgaatcagc tggtggactg
     2341 tgaatgtgct gctattggtg tcgttgctgg cgctggcaca ttctacagcc tgggaatatt
     2401 cggtgcgccg gtatctgagc gggttttcgg atgcgatcgt gccggctggc gccataccgg
     2461 aagaaagagt ggaggcgatt ctgaactgga tgcgggaggg acctccgaga ttggagggat
     2521 cgaccccgac ggggctggca atacgcgacc cggaaaagac gctgaattac cggcaattat
     2581 tgaccgtttg cgggacggcg acgaatgcgt ttctgaatct agcgcgaagt tccgacttgc
     2641 aggtacggcg cctgctgctg ttgacgccct accggaatac caagcacgtg gtagcggaag
     2701 tgcagttgaa cggacgatgg gtggtggtgg atccgacgta ccgggtaatc ctgcggaatg
     2761 gcgcgggcga aatggtcaca ctcagcgaac tgaagaatcc gcaaacgttt gccgaggtga
     2821 tcgggagagt gcccggctat ccggcggaat acaactatca aaactatgcg cacgtgcggg
     2881 tagcgcggtt gccgatgcaa gggtggcagc tgcggccgtt gctggatcgt gtttttccag
     2941 gttgggagga gagactggat tggagcctgt tgttggagcg agcgtcattt ttcgcgctga
     3001 ccctgtcggc gtgcgcgacg ttgattctgc ttgggctcag gctggtcatg ggctggtacg
     3061 ccgaccgaaa attgaagatc ccgaggtttc gcctgcgcgc gcacctggcg cgagcggggg
     3121 cagcgtttct tacatcgcct gagataaaat agcggcgtgt gtggaatctg cggcgtcgca
     3181 ttcctgaaca ctgcaaaaga tgctgaatcg cgggtgcgcg cgatggcggc ggcgatgcgc
     3241 catcgcgggc cggacggcga aggatttttg atcaatgatc ggcgagcgcc ggggttggcg
     3301 ctggcgatga ggcggctgag catcattgac ctggccggcg ggcagcaacc catctggaac
     3361 gaaacgctcg atgtgggagt tgtgttcaac ggcgagttgt acaactaccg cgagctgcgc
     3421 gagcggctga cgcgctgcgg gcatcaattc acaacccatt cggatacgga aatcctggta
     3481 cacgcatg
//
LOCUS       DASTPM010000276         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_614949, whole genome shotgun sequence.
ACCESSION   DASTPM010000276 DASTPM010000000
VERSION     DASTPM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614949"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..577)
                     /locus_tag="VFR42_04890"
     CDS             complement(59..577)
                     /locus_tag="VFR42_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0368519.1"
                     /translation="MPARKKQLPAKKIAADQSLRKHLVALLRGGEAHIQFADALADFP
                     SNKRGAFAAGLPHTGWQLLEHARIAQWDILEFSRNPKHVSPDFPEGYWPKTPGPPDGA
                     AWNKSMKGFQRDLQAMVALISNVRVDLHAPLPSGGGQTLLREALVLADHNAYHLGQLV
                     DLRRALKIWPES"
     gene            complement(583..1602)
                     /locus_tag="VFR42_04895"
     CDS             complement(583..1602)
                     /locus_tag="VFR42_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0368520.1"
                     /translation="MSSAVAPKPSPQLIFDSLNSYQHAFALKAAIELDLFTAIAEGAT
                     DAAAIAKRVNAAERGVRILADGMTVQGFLRKENGKYSLSPDSALFLDKRSPAYMGGMA
                     EFLVSAENIANFSQFTDCVRKGGTVSSIGDNSKPVDHRWVTFAKAMGSMAGPMAGVLS
                     QIVNSNPAPEIKVLDIAAGHGMYGITVAKNNPRVHVTALDWPAVLEVAKENAQKAGVS
                     DRYSLCPGSAFDADLGSGYDYVFITNFLHHFDIPTNEKLLRRFHAALKPGGKAITVEF
                     VPNDDRVSPPMAAAFSMVMLINTDAGEAFTFPEYESMCRNAGYKNATLQTIPDLPQRL
                     VIAEK"
     gene            1822..2553
                     /locus_tag="VFR42_04900"
     CDS             1822..2553
                     /locus_tag="VFR42_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0368521.1"
                     /translation="MKKEVHRWYSHRLGLELGVVVYGHWGAPLLGFPTSAGDEWELEG
                     QSLISALGDFIDGGKIKFYTVNSINGLSFYNQGAHPFHRSYVQSVFDAYLREEVVPFI
                     WDNCQSPGLAISTMGASFGAYHAANSLFKHPDVIKRCFALSGVYDLKNFMDGMYDDNF
                     YFNNPVDYIASLSDPWVYGQLASCDIHLSTGTGQWENSGPTYHFSGILASKGIRHSLD
                     NWGPMGGHDWPYWKHQMREYISHLF"
BASE COUNT          542 a          667 c          935 g          605 t
ORIGIN      
        1 attcggccgt gccattcgac gggccggcaa agttttgccg acggtccttt cgcgtgaatc
       61 agctttccgg ccagattttg agggcgcgac ggaggtcaac gagttggccg aggtggtagg
      121 cgttgtggtc ggcgagaacg agggcttcgc ggagcaaggt ttgaccgccg cccgagggga
      181 gcggggcgtg caggtccaca cggacattgg aaatcagcgc gaccattgct tgcaaatcac
      241 gttgaaaccc tttcatgctt ttgttccatg cggcgccatc cggtggcccg ggcgtttttg
      301 gccagtagcc ctccggaaaa tcgggcgaaa cgtgtttggg attgcggctg aattcgagga
      361 tgtcccactg ggcgatgcgg gcgtgttcga ggagttgcca gccagtatgg ggaagacctg
      421 cggcgaaggc gccgcgttta ttagacggga aatctgcaag cgcgtcggca aattgtatgt
      481 gggcttcgcc gcctcgtaac agggcgacga gatgcttgcg gagtgattga tcggccgcaa
      541 tcttttttgc tggcagctgc ttcttccgcg cgggcatggc ggctactttt cggcaataac
      601 cagacgctgt ggcaagtccg gaatggtctg aagggtggcg ttcttgtagc cggcgttgcg
      661 gcacatgctt tcgtattccg gaaaagtgaa ggcttcgccg gcatcggtgt tgatcaacat
      721 gaccatgctg aaggcggcgg ccatgggcgg actgacgcga tcgtcattgg gaacaaattc
      781 aaccgtgatg gctttgcctc ctggcttcag ggccgcgtgg aagcggcgga gaagcttctc
      841 attggttgga atgtcgaagt gatggagaaa gttggtgatg aaaacgtagt cgtagccgga
      901 gccgaggtcg gcgtcgaagg cactgccagg acagagcgag taacgatcgg agacgccggc
      961 cttttgagca ttttccttgg cgacttcgag aacggcgggc cagtccagcg cggtgacatg
     1021 aacccgcgga ttatttttgg cgacggtgat gccgtacatg ccgtgaccgg cggcgatatc
     1081 gaggacttta atttcggggg cgggattgga attgacgatt tgcgatagga cgccggccat
     1141 cggaccggcc atggagccca tggccttggc gaaagtgacc cagcgatggt cgaccggctt
     1201 gctgttgtcg ccgatgctgc tgacggtgcc gcctttgcgg acacaatcgg tgaactgaga
     1261 gaaattggcg atgttttcgg cactcactag gaattcggcc atgccaccca tgtatgcagg
     1321 cgagcgcttg tccaggaaga gtgcggagtc aggagagagc gaatactttc cgttttcctt
     1381 gcgcaggaat ccctgaacgg tcatgccatc ggcgagaatc cggacgccgc gttcggcggc
     1441 attgacgcgc ttggcgatgg cagccgcgtc ggtcgcgcct tcggcgattg cggtgaagag
     1501 atcgagttcg atggcggctt tcagggcgaa agcatgctgg taggaattga gagagtcaaa
     1561 aatcaattgg gggctgggtt tgggcgcgac ggcagaagac atgaaaagct cctttagaag
     1621 aatagcgagg ttctggcgat ccgaggagcc cgcaaaaagg ggggctggaa tcgagcgcat
     1681 cctttatacg cctgctgaga gatccaggaa agagtttccg gagtcggcag aagcccagtg
     1741 gcgcggtgtc gatgcggagt tgcgccgtgc cggggccgct ggcatactca cgcttctcca
     1801 aaattgcggg aagggcgcgt gatgaaaaag gaagtacatc ggtggtattc gcatcgcttg
     1861 ggactggaac tgggcgtagt ggtgtatgga cactggggcg cgccgttgct gggatttccg
     1921 acgagcgcgg gcgacgagtg ggagctggaa gggcagtcgc tgatcagtgc gctgggcgat
     1981 ttcatcgacg ggggcaaaat caagttttac acggtgaatt cgatcaacgg gcttagcttt
     2041 tacaaccagg gtgcgcaccc gtttcatcgc agctatgtgc agtcggtgtt tgacgcgtat
     2101 ttgcgcgagg aagtggtgcc gttcatctgg gacaactgcc agtcacccgg gctggcgatt
     2161 tcgacgatgg gcgcttcgtt tggcgcgtac catgcggcga attcgctgtt caagcacccc
     2221 gatgtgatca agcggtgctt tgcgctttca ggtgtgtacg acctgaagaa tttcatggac
     2281 ggaatgtacg acgacaattt ttatttcaac aacccggtgg actacatagc gagtttgagc
     2341 gatccgtggg tgtatgggca gctggcgagt tgcgacattc atttatcgac ggggaccggc
     2401 cagtgggaaa attccgggcc tacatatcat ttttcgggaa ttctggccag caaagggatt
     2461 cggcactcgc tggataattg gggaccgatg ggcgggcacg actggccgta ctggaagcac
     2521 cagatgagag agtacatttc gcatctattt tgagtgccgg gctcgcgtac gcgcggcttt
     2581 ttggctcgcg cgtcgtaagc cgctcttgcc cttggtccgg acagctttca tcgcagcctc
     2641 atgcattgaa gagtgcccgc gtcggcgcgc tgcgctccgc gtttgccggg acaatgcgga
     2701 atgagaggcc gagctgtggc gctcgcgctt taaagcgttt gtggtcgca
//
LOCUS       DASTPM010000277         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1076398, whole genome shotgun sequence.
ACCESSION   DASTPM010000277 DASTPM010000000
VERSION     DASTPM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076398"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFR42_04905"
     CDS             complement(<1..846)
                     /locus_tag="VFR42_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0368522.1"
                     /translation="MALLLFAVTFLTCLAAGRQFAISFANGHAASIDEFVVSLKLLYK
                     DPTALGPGIPFAAALMSILLAHELGHYFACRYHGIRATYPYFIPAPTPIGTFGAFILM
                     RSAIRSRRALFDVGASGPLVGFVVAIPLLIYGVAHSHVVAGLASRDGEWIFGVPLALR
                     FVESTFFPGVHPGAILLHPVARAAWVGLLATSLNLLPAGQLDGGHILRSLSTRLHKWS
                     GLAVPLVLIALWFLNPTAKVWLLWAGILLALRILRIPPVYDERPLDPVRVVLAAASLL
                     VMILCF"
     gene            complement(1027..1326)
                     /locus_tag="VFR42_04910"
     CDS             complement(1027..1326)
                     /locus_tag="VFR42_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0368523.1"
                     /translation="MAEERLIKLDIVNAVLAKLEGASISRVKAEQAVETVFESLKNGM
                     GRSQRIELRRFGVFTVKPRKTGIGRNPRTGEEVNIPPGKAVRFKPGKELQGLGEQ"
     gene            1569..>2067
                     /gene="dcd"
                     /locus_tag="VFR42_04915"
     CDS             1569..>2067
                     /gene="dcd"
                     /locus_tag="VFR42_04915"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU0368524.1"
                     /translation="MSVKPDKWIRRMSLEHKMIEPFTDRQVREGVISYGVSSYGYDIR
                     VADEFRIFTNVNTTIVDPKNFDPRSLVEFKGEVCIVPPNSFALARSIEYFRIPRNVLT
                     VCVGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGLCQVIFFESDE
                     DCEISY"
BASE COUNT          476 a          659 c          510 g          422 t
ORIGIN      
        1 aaaacacaaa atcatcacga gcaacgatgc cgctgccaac acgacgcgga ccggatccag
       61 cggccgctcg tcatataccg gtggaattcg caaaattcgc agcgccagca gaattcctgc
      121 ccacaataac cacaccttcg cggtggggtt cagaaaccac aatgcgatca acaccagcgg
      181 cacagccagt ccgctccact tatgcagccg cgtactcagc gaacgcaaaa tgtgcccgcc
      241 gtccagctgc cccgccggca ataaattcag cgacgtcgcc agcagtccta cccacgcagc
      301 ccgcgccacc ggatgcagca gaatcgcgcc agggtggact cccggaaaaa acgtgctctc
      361 cacaaaccgc aatgctagcg gcacgccaaa gatccactcg ccatcccgag aagccagccc
      421 cgccaccaca tgcgaatgtg ccacgccata gatcagcagc ggaattgcca ccacaaatcc
      481 tactagcggt cccgacgctc ccacatcgaa cagtgctcgc cggctgcgaa ttgcagaccg
      541 catcagaatg aacgcgccaa acgtacctat cggcgttggt gctggaataa agtacggata
      601 ggtcgcacga atcccgtgat acctgcacgc gaaatagtgt cccaactcgt gcgccagcag
      661 gatgctcatc agagcagccg caaacggaat tccgggcccc agtgccgtcg gatccttgta
      721 cagaagtttc aacgaaacaa cgaactcatc gatggaagcc gcgtggccgt tggcgaatga
      781 aatcgcgaat tgccgtcccg ctgccaggca ggtaagaaac gtcacggcaa aaagcagcag
      841 cgccaaccac aatcgcttcg gtctttgctt gggaggctgc caaacgggaa cggctggagg
      901 aaagtcttcg attcgtgatc gcgtctctgc tagctcgatg gtgtgcggtg gaaactgatt
      961 ttcactgttc acaaaacttg gatgcgggtc tttcttctac ggtgagccac aaagaatctt
     1021 ctcaaactat tgttcgccca ggccttgcag ttccttgccg ggcttaaaac gcactgcctt
     1081 acccggtggg atattgactt cttcgccggt gcgcgggttg cgcccgatgc cggtctttct
     1141 cggcttcacg gtaaacacgc cgaagcgccg cagctcgatc cgctggctgc gccccatgcc
     1201 gttcttcagc gactcaaaaa ccgtttctac cgcctgctcg gccttcacac ggctgatgct
     1261 cgcgccttcc agtttggcca gcaccgcgtt tacgatgtcc agtttaatca agcgttcctc
     1321 cgccaatccc tgttgcagat tcctcgcgct gcttctgcaa atcccgcgct gttatcggga
     1381 cattcgcgcc gtgcccttac cttagcgaat tccgctggca gatgctagag gcaccttgtc
     1441 aggttgtcaa gacgttgtgg ctcgtctaca ctgactgttc ttcacgcaac atacaacttg
     1501 ttttcgtcgt gcggttcgcg aactcaatca tgaaattgca tttcacattc gcgccgcaag
     1561 gagaaatgat gtccgttaag ccggataagt ggattcgccg catgtctctc gaacacaaaa
     1621 tgatcgagcc tttcaccgac cgccaagtcc gcgaaggcgt gatcagctac ggcgtctctt
     1681 cgtacggcta cgacattcgt gtggccgacg aattcaggat cttcaccaac gtcaacacca
     1741 ccattgtcga ccccaaaaat ttcgatccgc gctcgctcgt ggaattcaaa ggcgaggttt
     1801 gtatcgttcc cccgaactcc tttgctctcg cgcgctctat cgaatatttc cgcatcccgc
     1861 gcaacgtgct gacggtctgc gtcggcaagt ccacctacgc gcgctgcggc atcatcgtca
     1921 acgtcacgcc gttcgagccg gagtgggaag gctatgtcac gctcgaaatc agcaacacca
     1981 ctccgctgcc cgcaaagatt tacgccaacg aaggactttg ccaggtcatc ttcttcgagt
     2041 ccgacgaaga ctgcgaaatc agctaca
//
LOCUS       DASTPM010000278         6091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_557830, whole genome shotgun sequence.
ACCESSION   DASTPM010000278 DASTPM010000000
VERSION     DASTPM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557830"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..959)
                     /locus_tag="VFR42_04920"
     CDS             complement(51..959)
                     /locus_tag="VFR42_04920"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0368525.1"
                     /translation="MSIRPVKRLIKSKPTMEGAGVHLRRAFGFGNTAEFDPFLLLDDF
                     RNDVPEDYLKGFPWHPHRGIETITYVLAGTVEHGDSIGNHGAITPGDVQWMTAGRGII
                     HQEMPKGDAAGRMHGFQLWANLPSALKMTPPRYQEVKSNDIPQITDDDGTHVRVICGH
                     FWGKSGPVDGIAAEPVYLDISLAPGRRKALPVGTTRHAFAYVFAGSGKFCNASNPLAV
                     PTEPVGWLDTAPPAEADNRSLVLFDRGDEVVVQAGEEGIRFLLVSGKPLEEPVAWYGP
                     IVMNTQEQLQQAFRELEKGTFLKSGT"
     gene            1090..2370
                     /locus_tag="VFR42_04925"
     CDS             1090..2370
                     /locus_tag="VFR42_04925"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0368526.1"
                     /translation="MKRLGVELLLAVGLVLASLAAAAQDVPTAKPESVGLSSERLERI
                     TAKVQQAIDDKRIAGAVTLVARHGKIAYFKPQGSLDREANKAMRNDAIFRICSMTKPI
                     TSVAVMMLYEEGRFLLEDPISKYLPEFKNAKVYVKPAQGEAYTIPAAREITILDLLTH
                     TSGLTYDWNETLGEQYKAANVATGLLQYDGTIEDNVKGLATVPLLFSPGTRWEYSLGV
                     DVLGRLVEVASGMPLDRFFRTRIFEPLGMKDTYFYLPDNKVDRLAVAYTYYSDKGLQR
                     FPDAPITEGSFRYSADYPYRGPKKLFAGGAGLSSTAADYYRFCQMMLQGGKLGDVRLL
                     SRKSVELMTHDQLGRISDEQAFGLGFGIDGVKTPLKELSSPGTFAWGGFFYTAFTIDP
                     KEDMIVIFMGQLHPTGGLTLDSQVHALAYQAIVD"
     gene            2439..2858
                     /locus_tag="VFR42_04930"
     CDS             2439..2858
                     /locus_tag="VFR42_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0368527.1"
                     /translation="MRPGQALAGSGLENKLLLLIKMRASQINGCAYCLDMHSIDARAE
                     GESEQRLYTLNAWAETPFFSDRERAALAWTEAVTNVSQTHVPDEVFEEVKKHFSEKEI
                     VDLTLALAMINLWNRIAISLRAVPGHYRAAVKSSAAS"
     gene            3021..4718
                     /locus_tag="VFR42_04935"
     CDS             3021..4718
                     /locus_tag="VFR42_04935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU0368528.1"
                     /translation="MRLRTIFLSLGFSLLSLAAFESDGAAQEPTIAQAIDKIVTQEQA
                     EVQSLRRYSPLVETYIQLVRPDKDLGAVPDGDRYFLGRAALAKGVELEPLVTNNGTST
                     AKQKLFSTIGNFFSAEFLPRGFLQMIYLDMDGFDRQHYKFTYAGREFLGEVRCLVFDV
                     DPLPNTGKGRFAGRIWAEDEGYHIVRFNGAYGGSSRGSYYFNFDSWRVNTGKNEWLPA
                     YIYSEEGNVQYGVAKHTAFRAFKSQTRLWGYDLDQSKEEQALSSVSIEAPKINDQSQK
                     GNGYSPLQARRAWGRLAEDNVTAKMQALGLLAPYGDVDRILETVVNNLEVTNELDIQP
                     DVRCRVLMTSTLESFTLGHTIVVSRGLIDVLPDEASLAAVLAHELAHVTLGHRMDTQF
                     AFFNRTRFDEKETFHHFGFARNNEEEEAANQKAVVLLKKSPYVDQSGTAQAFFLALQS
                     RAKEIPNLISPHLGDRVPTNWTLADTASVQTASAKLGAATTPALPLGGRIKVEPWNDQ
                     LTLLKPQAEGGAVAEYEKMPFEVAPFLVYLTRQDAASKLTSRPTVINAEAVVDPSRQ"
     gene            4944..5417
                     /gene="msrA"
                     /locus_tag="VFR42_04940"
     CDS             4944..5417
                     /gene="msrA"
                     /locus_tag="VFR42_04940"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU0368529.1"
                     /translation="MTELATFAAGCFWGVEVKFRMIPGVVDAAVGYSNGHTENPTYKD
                     VCTDETGHAEVVQVTFDPAKVSYETLVDAFWQMHDPTQMNRQGPDLGKQYRSAIFTHS
                     PEQEAIAHKSKIALEASKKFHRPIATEITPAGTFYRAEEYHQRYLEKRGMATCHL"
     gene            5508..>6091
                     /locus_tag="VFR42_04945"
     CDS             5508..>6091
                     /locus_tag="VFR42_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368530.1"
                     /translation="MAHAKTPIAKTPMLLAAFIFLATASPAQIPHQPTVPSIQLSHIL
                     SADRAEFVAQDKKVIMKKGEASDRWTLVELIPVADNAASSYAVLEDYTQINGRILFVD
                     SAGVRLDFPKSSEPTSSDPAKLYLGHSREEVKQSPTDLLAREILARPGDPKYEEVAAV
                     FEPLRKMQTYSFVGTPNTMDKIGFVYGGRSPNFDP"
BASE COUNT         1351 a         1891 c         1589 g         1260 t
ORIGIN      
        1 actgcccact gcgcaagcct caccgaattt cattccgagc gcttccgtta ctacgtccca
       61 ctttttaaaa acgtgccctt ttccagttcg cgaaacgcct gctgcaactg ctcctgggtg
      121 ttcatcacga tcggtccgta ccacgccacc ggttcttcca gcggttttcc ggataccagc
      181 agaaagcgaa ttccttcctc gcccgcctgt acgacaactt cgtcgccgcg atcgaacaac
      241 accagcgaac gattgtccgc ctccgctgga ggagccgtat cgagccagcc gacaggttcg
      301 gtaggcacgg ccagcggatt ggacgcgttg cagaattttc ccgagccggc aaacacatat
      361 gcaaacgcat gccgcgtcgt gccgaccggc aacgctttgc gccgccccgg cgcaagtgaa
      421 atatccagat aaacaggctc cgccgcaatt ccatccaccg gaccgctttt gccccagaag
      481 tgtccgcaga tcacgcgcac gtgcgtcccg tcatcgtcgg taatttgcgg aatgtcgttc
      541 gacttcactt cctgatagcg tggcggtgtc atcttcagcg ctgacggcaa gttcgcccac
      601 aactgaaagc cgtgcatacg gcctgccgca tcacccttgg gcatttcttg atgaatgata
      661 ccgcggcccg cggtcatcca ttgcacatcg cccggcgtta tcgcgccatg attgccgatg
      721 ctgtcgccat gttcgacggt tcccgccagc acgtaggtaa tcgtttcaat gccgcggtgc
      781 ggatgccacg gaaaaccctt cagataatct tctggcacat cattgcggaa gtcatcgagc
      841 aacaggaaag gatcgaactc tgcggtatta ccgaagccaa atgcgcgtcg caggtgcact
      901 cccgcgcctt ccatggtggg cttggatttg atgagacgtt ttaccggtcg gatggacata
      961 cggacctcct gtgctccccc tcggaattta gcacaaattg caatttgcct ttcgaggagt
     1021 cgtccgttgt agaattcccg ccataacgca aggtcatctt ccatgaggtg caagatgcaa
     1081 cgaaaaacta tgaagagact tggagtggag ttgcttctcg cagtcggcct ggttttggcg
     1141 agcctcgccg cggcagcgca ggatgtacct accgccaagc ccgaatcggt cggcctctcc
     1201 tcggagcgcc tcgaacgcat caccgcgaag gtgcagcaag cgattgacga caagcgcatc
     1261 gccggagccg tcacactcgt cgcgcgtcac gggaaaattg cgtacttcaa gccgcaggga
     1321 tcgcttgatc gcgaagccaa caaagcgatg cgcaacgacg cgattttccg catctgctcc
     1381 atgactaagc ccattaccag cgtcgcagtg atgatgcttt acgaagaggg ccgcttcctt
     1441 ctcgaagatc ccatttccaa atatttgcct gaattcaaga atgcaaaggt ctacgtcaag
     1501 cctgcccagg gcgaggcgta caccattccg gccgcgcgcg aaatcaccat cctcgatttg
     1561 cttacgcaca cttccggcct cacctacgac tggaacgaaa cgctgggcga acagtacaaa
     1621 gcggcaaacg tcgcgaccgg cctgctgcaa tatgacggca caattgaaga caacgtaaag
     1681 ggcctcgcga cggttccgtt gctgttcagc cccggcacgc gatgggagta cagcctcggc
     1741 gttgatgttc ttggccgcct ggtcgaagtc gcctccggca tgccgcttga tcggtttttc
     1801 cgcacccgta ttttcgagcc gctgggcatg aaggacacct acttctactt gccggacaac
     1861 aaagtggatc gtctcgctgt cgcttacacc tactattcgg acaagggcct gcagcgcttt
     1921 ccggacgcac ccatcaccga aggctcgttc cgttattccg ctgactatcc ttaccgcggc
     1981 cccaaaaaac tctttgcagg tggcgcaggt ctcagctcga ctgcagccga ttactaccgt
     2041 ttttgccaaa tgatgctaca gggcggcaag ctcggcgacg tacgcctcct gagccgcaaa
     2101 tccgtcgagc taatgacgca cgaccagctc ggccgcatca gtgacgagca ggcgtttggc
     2161 ctcggcttcg gcatcgacgg cgtaaagacg ccgctcaaag agctaagttc accgggcacg
     2221 tttgcgtggg gcggattttt ctacaccgcg ttcaccatcg atcccaaaga ggacatgatc
     2281 gtgattttca tgggccagct gcacccgacc ggtggcttga cgctggacag ccaggtccat
     2341 gcgctggcct atcaggccat cgtcgactga cgcttcttcg tccgctcggg cgcctgattt
     2401 ttttgtataa ctccgccgtg gctcgttcat ctatttcttt gcgacccggc caagcattag
     2461 cgggcagcgg gctcgaaaat aagctgctgc tcttgataaa gatgcgcgcc tcgcaaatca
     2521 acggctgcgc atattgtttg gacatgcact ccattgacgc gcgcgccgaa ggcgagagcg
     2581 aacaacgcct ctacacgctg aacgcctggg cggaaactcc gttcttctct gatcgcgaac
     2641 gtgctgcgct cgcctggacc gaggcggtca ccaacgtttc acagactcac gtacccgacg
     2701 aagtgttcga agaagtaaaa aagcatttca gcgaaaaaga aattgtcgac ctgaccctcg
     2761 cgctggccat gatcaatctc tggaatcgca tcgccatttc gctgcgcgcg gttcccggcc
     2821 actatcgcgc cgccgtcaaa tcgtccgccg cgtcctgacg gtcttgccat caggcgcaaa
     2881 tttaccgcag aattctcgtg gcagcctgtc aatacagacg gctgtcccaa aactgttctt
     2941 tcgagcagct gccagcaacg tccccgccag ctaacctccg acgtcgagat atcctaaacg
     3001 ttcaccagca cggggtaatt atgcgtcttc ggaccatttt tctttctctc ggattttccc
     3061 tattgagttt ggcagcattc gagagtgacg gggcggcgca ggagcctacc atcgcgcaag
     3121 ccatcgataa aattgtcacg caggaacaag ccgaggttca gtcgctgcgg cggtattcgc
     3181 cgcttgtcga aacgtatatt caactggtgc gtcccgacaa ggatttgggt gccgtgcctg
     3241 atggtgaccg ctattttcta ggccgcgccg cgctcgccaa aggtgtggag ctggagccgc
     3301 tggtcaccaa caacggaacc agcaccgcaa aacaaaagct gttctccact atcggcaatt
     3361 ttttctcagc tgagttcttg ccgcgcggct tcttgcagat gatctatctg gatatggacg
     3421 gtttcgatcg ccagcattac aagttcacct acgcagggcg cgaatttctg ggcgaagtcc
     3481 gctgcctggt ctttgacgta gacccgctgc ccaacaccgg caagggccgc ttcgccgggc
     3541 gcatctgggc ggaagacgag ggttaccaca tcgtgcgttt caacggcgcg tatggcggtt
     3601 cgtcgcgcgg cagctactac ttcaattttg acagttggcg tgtgaacacg ggcaagaatg
     3661 aatggctgcc ggcctacatc tacagcgaag aaggcaacgt acaatacggc gtagccaagc
     3721 acaccgcgtt tcgcgcgttc aaatcgcaaa cgcgtctctg gggatacgac ctggaccaat
     3781 cgaaggaaga gcaggcactg agtagcgttt ccattgaagc gccaaaaatt aacgatcaat
     3841 cccagaaggg caacggatac tcgccgctgc aagcccgccg cgcgtggggt cgcttggctg
     3901 aggacaacgt cacggcgaag atgcaggcgc taggcttgct cgctccttat ggcgacgttg
     3961 accgcatcct tgaaaccgtg gtcaacaatc tcgaagtcac caacgagttg gacattcagc
     4021 ccgacgtccg ttgccgtgtg ctcatgacgt ccacgctgga atcattcaca cttggccaca
     4081 cgatcgtagt tagccgcgga ctcattgacg tcctgcccga tgaagccagt ctcgcggcgg
     4141 tgcttgcaca tgaactcgcg cacgtcaccc tcggccaccg aatggacaca caatttgcgt
     4201 tcttcaaccg cacgcgcttc gatgaaaagg aaacctttca ccatttcgga ttcgcgcgca
     4261 acaacgaaga agaggaagcc gctaaccaaa aagcggtggt gttgctgaaa aagtcgccat
     4321 acgtcgatca atccggaacc gcgcaggcgt ttttcctagc attacaaagc cgcgccaagg
     4381 aaattcctaa tctgatcagc ccgcatctgg gcgaccgcgt accaaccaac tggacgctgg
     4441 ccgacaccgc ttccgtccag acggcctccg caaaactggg cgccgcgaca acgcctgcgc
     4501 tgccgcttgg cggccgcatc aaggtcgaac cctggaacga tcaacttacc ctgctcaagc
     4561 cgcaagccga aggcggcgcg gttgccgaat acgagaaaat gccatttgaa gtcgcgccct
     4621 tcctggttta cctgacccgg caggatgccg cctccaaact cacatccagg cccacggtaa
     4681 tcaacgcgga agccgtcgtc gaccccagcc gtcaataacc ttggcatcag ctccaatcga
     4741 tctgaacgtg cccgccgcat gactgggcgc gttcatttcc gctcgcggtt tcttgccgcc
     4801 gtaaccctca gaaacctcgc cgattaggag ccttcggcag tcctagcccc tgcctggagc
     4861 ccggaaacct ttcgcgccgc cacgcatcag atgggcaaac caaatcgagt tacaatgaag
     4921 ctgaaagaca ggaaggaaat cccatgactg aactcgcaac ctttgccgca ggatgtttct
     4981 ggggagtcga ggtgaaattt cgcatgatcc ccggcgtggt cgacgccgca gtaggctaca
     5041 gcaacggcca cacagaaaat ccaacataca aagacgtctg caccgacgaa actggccacg
     5101 ccgaagtcgt gcaagtaacc ttcgacccgg ccaaggtcag ctacgaaacg ctggtggacg
     5161 ccttctggca gatgcacgat ccaacgcaaa tgaaccgcca gggccccgac cttggcaagc
     5221 agtatcgttc cgcgattttc actcattcac cggagcagga agcgatcgcc cacaagtcca
     5281 agattgctct cgaagccagc aagaagttcc accggcccat cgcgaccgaa attactcccg
     5341 ccggcacgtt ctatcgtgcg gaggagtatc accagcgcta tctcgaaaag cgcggcatgg
     5401 ccacctgcca tctatgatcg ccggttcgac ttaacccacc gcaaaaatcg attgctcaac
     5461 ttagacggcg cccgcatact tgggcgccgc ctttctttgg aggagcattg gcgcacgcga
     5521 agaccccgat cgcgaagacc ccgatgttgc tggctgcatt cattttcttg gccaccgcat
     5581 cccccgctca gattccgcat cagccaacag ttccgtccat ccagctctct catattcttt
     5641 ctgcggatcg cgccgagttc gtcgctcaag acaagaaagt catcatgaag aaaggcgaag
     5701 cctctgaccg ttggacgctg gtggaactaa tccccgtcgc ggacaatgca gcatccagct
     5761 atgcggtcct cgaggactat acccaaatca atggccgcat cctttttgtc gattcggccg
     5821 gcgtccgtct ggattttccg aaatcctcgg agccaacttc gtccgatccc gcaaaattgt
     5881 atctaggcca ttcgcgggaa gaagttaagc agagcccaac ggatttgcta gctcgcgaaa
     5941 ttttggcccg gcctggtgat ccgaaatacg aggaagtcgc cgccgttttc gagccgctgc
     6001 gcaaaatgca aacctacagc ttcgttggaa cgcccaacac catggacaag attggctttg
     6061 tctacggcgg tcgctcgcct aatttcgatc c
//
LOCUS       DASTPM010000279         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1076428, whole genome shotgun sequence.
ACCESSION   DASTPM010000279 DASTPM010000000
VERSION     DASTPM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076428"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="VFR42_04950"
     CDS             complement(<1..1229)
                     /locus_tag="VFR42_04950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368531.1"
                     /translation="MPDMLQAPEGRVTGRAKPSRIFDFPRDITSPERKSLLAGGFGWM
                     LDAMDVMLYSLVLTYLIREFSMDTRTAGFLNSLTLVASAIGGLLFGIVADRIGRTRSL
                     MISILVYSLASAACAFSHSVSQLAAFRFVLGLGMGGEWTAAAALIAETWRPEHRGKAL
                     GLMQSAYAIGEAIAALVVGLVLPSFGWRAVFLVGVLPALLVFWIQASVPESTLWKTLR
                     SAHTERPPLRQLLSGKVLRTGLLATTMNTCALFGYWGLFTWIPAYLSLPPAQGGRGLS
                     LVKTTTFFLVLCAGKFLGYSAFGFFADAFGRRKPYFGYLIIAAALVPIYGMTSSPTLL
                     LLLGPLVAFFGTGFFSGYAAIASEIFPGEIRAAAMGLSYNVGRGISAVAPFAVGALAV
                     THGIGPAFLLQAGAFFVA"
     gene            1414..2373
                     /locus_tag="VFR42_04955"
     CDS             1414..2373
                     /locus_tag="VFR42_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368532.1"
                     /translation="MTRVRLCFHDRCFDGTASAALFYRFYRERFDPSAEFYFSGMAHR
                     ASHPWEGGILDGDENVIVDFKYSNSPKLTWWFDHHQSAFLTPDDARHFREHRGAHMFY
                     EPDYRSCTKLIATVGKEKFGFDTRPLENLIHWGDIIDGAQYPTPESAVEIAEPATQLA
                     LVIEAAPEEGLPARIIPELAYRPLEEMIQLPLVAKHLGPLLERHRRSIDILRQRAEAR
                     DNVIFFDVSDLDLEGYNKFIPYLLHPECVYSISVSASAMRAKVSLGTNPWNQATADKN
                     LASLAEKFGGGGHPRVAAISFEPGDLPRAREVARELTAVLRAG"
BASE COUNT          532 a          737 c          736 g          456 t
ORIGIN      
        1 gctacaaaga aggcgccggc ctgcagcagg aacgcggggc caatgccatg cgtaaccgcc
       61 agcgccccca ccgcaaatgg cgccacggcg gaaattccgc gtccaacgtt gtaactcaac
      121 cccatggcgg ctgcgcgaat ctcgccgggg aagatttcgc tggcgatggc cgcataacct
      181 gaaaagaatc ctgtaccaaa gaaagccacc aatgggccga gcagcagcag caacgtcggg
      241 ctgctggtca tcccatagat gggcaccagg gcggcggcga ttatgaggta tccaaaataa
      301 ggttttctcc gcccgaaggc gtccgcgaag aatccaaacg cgctgtatcc aagaaatttt
      361 cccgcgcaaa gaaccaggaa aaaagtggtt gtcttcacca ggctgagccc gcgtccacct
      421 tgcgccggag gcagcgacag gtacgcaggg atccacgtga acaagcccca gtagccgaaa
      481 agggcgcaag tgttcatggt ggtcgccagc aagcctgtgc gcaaaacctt tcccgagagc
      541 aactgccgga gcggcggtct ttctgtgtga gcggagcgga gtgttttcca caaagtggat
      601 tccgggacgc tcgcttgaat ccagaagacc agcagggccg ggagcacgcc aaccagaaaa
      661 accgcgcgcc agccaaagct cggaagcacc aggcccacga ccagggcggc aattgcttcg
      721 ccgatggcat atgccgactg catcagcccg agcgccttac cgcgatgctc cgggcgccac
      781 gtttcggcaa tcagagcggc ggccgcggtc cattcgccgc ccatgcccaa cccaaggaca
      841 aatcggaacg ccgccaattg cgagaccgaa tgcgaaaacg cgcacgctgc gcttgccagg
      901 gaatagacca ggatggagat catcagcgaa cgcgtgcgcc cgatgcggtc cgccacgatg
      961 ccgaaaagca ggccgccgat tgccgaggcc accagggtga gtgagttcag aaagccggct
     1021 gtccgcgtgt ccattgagaa ttcgcggatg agataggtga gcaccagcga gtacagcatc
     1081 acgtccatgg cgtccagcat ccagccgaag ccgcccgcaa gcagggattt gcgttccggg
     1141 gaggtgatat cgcgagggaa gtcaaatatt cgggacggct tggcgcggcc tgtaacccgg
     1201 ccttcggggg cttgcagcat gtccggcatt gcggcggatt gtagccgaat agaggctctg
     1261 cgtgttgctc ggcggaatct gcgtagaacg ccggcagcac agtttcgtcg cgcgcatttc
     1321 cgcttgactt ccgaaagacc tacttgcatt gttaagagct ttgtccctcg aaaaatccgc
     1381 accgggggaa agaaaggcgc actccggctc gacatgaccc gcgtacggct ctgttttcac
     1441 gatcgctgct ttgatggcac cgcctccgcc gcgctctttt accgctttta tcgcgaacgt
     1501 tttgaccctt ccgctgagtt ttacttcagc ggcatggcgc atcgcgccag ccacccgtgg
     1561 gagggcggca tcctggatgg cgatgagaac gtgattgtgg acttcaagta ctcgaattcg
     1621 ccgaagctga cctggtggtt tgaccatcac cagagcgcgt ttctcactcc ggacgacgcc
     1681 cggcattttc gcgaacatcg cggcgcacac atgttttatg agcccgacta ccgttcctgc
     1741 accaagctga tcgccaccgt ggggaaagag aaattcggat tcgatacccg gccgctcgag
     1801 aacttgattc attggggcga catcatcgat ggcgcgcaat atcccactcc ggaatctgcc
     1861 gtcgagattg ccgagccggc cacgcaactc gctctggtga ttgaagccgc tccggaagaa
     1921 ggattgcccg cgcgcatcat tccagagcta gcctaccggc cgctcgagga aatgattcag
     1981 ttgccgctcg tggctaagca cttgggccca ttgctcgagc gtcatcgccg ctccatcgat
     2041 attcttcggc agcgcgcgga agcccgcgac aatgtaattt tctttgacgt gagcgatttg
     2101 gacctggagg gatacaacaa gttcattcct tatttgctgc atcccgaatg cgtctattcc
     2161 atcagcgtga gcgcgtcggc catgcgcgcc aaggtcagcc ttggcacaaa tccctggaac
     2221 caggcgacag cggacaagaa tctggcgtcg ctcgcggaga aatttggcgg gggcggccat
     2281 ccgcgcgtcg ccgcgatctc ttttgaaccc ggtgacctgc cccgcgcccg cgaagtcgcg
     2341 cgcgaactta ccgcggttct gcgcgcgggg tgagtttagg cggaagttgc gcgaagtatg
     2401 tagcgcgggc gtgcgttcga caaagttgca gagcgcatgt aaaattctaa ggttgtcttt
     2461 g
//
LOCUS       DASTPM010000280         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1018759, whole genome shotgun sequence.
ACCESSION   DASTPM010000280 DASTPM010000000
VERSION     DASTPM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018759"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="mtnB"
                     /locus_tag="VFR42_04960"
     CDS             <1..618
                     /gene="mtnB"
                     /locus_tag="VFR42_04960"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HEU0368533.1"
                     /translation="ALAARLAAIGREFHSRRWALGTSGNYSAVLSHAPLRLLITSSGL
                     DKSALCAKDFLEIDQHAAVLKGRGKPSAETSLHLAVVAVRNAGCVLHTHSVWSTLLSE
                     RYQPQGGLAFSGYEMLKGLEGVKTHLHREWIPIFANDQDIPALAARVEAELDRDPASH
                     GFLLSGHGLYTWGEDLAEARRHIEIFEFLFEVAGRSEFGTPPSQS"
     gene            641..1207
                     /locus_tag="VFR42_04965"
     CDS             641..1207
                     /locus_tag="VFR42_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0368534.1"
                     /translation="MASVTIPDEKKSLTQEAEVRDYLATIGITYEKWQPSHELLAGAS
                     AAEVLAAYAPEIDRLKDQGGYVTADVIDVSPATPGLDAMLNRFNSEHWHDEDEVRFII
                     EGRGLFHIHPREGKVVSITVEAGDLLRVPRGTWHWFDLCQEKRIRAIRLFQDSSGWTP
                     HYTESGADRHYDPVCFGPSYIAGRGAIS"
     gene            1223..1978
                     /gene="mtnC"
                     /locus_tag="VFR42_04970"
     CDS             1223..1978
                     /gene="mtnC"
                     /locus_tag="VFR42_04970"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR01691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HEU0368535.1"
                     /translation="MLPPFESAEIRALLLDIEGTTTPIDFVFKTLFPYAAANVEQFLR
                     QNQSDPEIKNLIAELRSTWSGAASSGAPVWIESSREEKLASAASYVRWLIARDSKITP
                     LKTLQGKIWEQGFRSGALKGEVYPDVAPAFARWRTQHKRIVTFSSGSVLAQQLLFGHS
                     TLGDLSSFIEAYFDTITGPKREPASYRKIAAALKLSPTAVLFVSDTGQELDAARDADL
                     RTALSLRAGNPSEITVVEQRHPAIHNFNELFAA"
BASE COUNT          436 a          742 c          516 g          437 t
ORIGIN      
        1 gcgctcgccg cacgactcgc cgccatcggc cgcgaatttc attcccgccg ctgggctctc
       61 ggcaccagcg gaaactacag cgcagttctc agtcatgctc ctctgcgtct gctgatcacc
      121 agttcgggac tcgacaagtc cgcgctctgc gccaaagact tcctcgaaat cgatcagcac
      181 gccgccgtcc ttaagggaag aggcaaaccc tcggccgaaa cttcccttca cctcgccgtt
      241 gtggccgtcc gcaacgccgg gtgcgtcctc cacacccatt ccgtttggag caccctgctt
      301 tccgagcgtt accagccgca aggcggcctg gccttctccg gctacgaaat gcttaagggc
      361 ctcgaaggtg tcaaaaccca cctccaccgc gaatggattc cgatttttgc gaatgaccaa
      421 gacatccccg ctctagccgc cagagtggaa gctgagttgg accgggatcc ggcctcccac
      481 ggattccttc tcagtggcca cgggttatat acttggggag aagacttggc cgaggcgcgc
      541 cgccacattg agattttcga gtttctgttc gaagtcgccg gcaggagcga attcggcact
      601 ccgccaagtc agtcttagcg atatccgacg aaggtgcgca atggccagcg taacgattcc
      661 cgacgaaaag aaatccctca cgcaagaagc cgaagtccgc gattacctcg ccaccatcgg
      721 catcacttac gaaaaatggc agccttcaca tgaacttctg gccggcgctt cggccgctga
      781 agtcctggct gcctatgctc ctgaaattga ccgcctcaag gaccagggcg gctacgtcac
      841 cgccgacgtg attgacgtat cccctgccac gcccggtctc gacgccatgc tcaatcgctt
      901 caactccgag cattggcacg acgaagacga agtccgcttc attatcgagg gccgcggcct
      961 gtttcacatt catccgcggg aaggcaaagt cgtttccatc actgtcgaag ccggcgatct
     1021 tctccgcgtt ccacgcggca cttggcactg gttcgatctc tgccaggaaa agcgcattcg
     1081 cgccattcgc ctatttcagg acagttccgg ctggactccc cattacactg aaagcggcgc
     1141 cgaccgccac tacgatcccg tctgcttcgg cccctcctac attgcgggcc gcggcgctat
     1201 cagttgatgc agcgagctga ctttgctgcc gccgtttgag tctgctgaaa tccgcgccct
     1261 tctcctcgac atcgaaggca ctaccactcc catagacttc gtcttcaaaa ctctcttccc
     1321 ctacgccgct gctaacgtcg aacaattcct ccggcaaaat caatccgatc cggaaatcaa
     1381 aaacctcatc gccgaactcc gttccacctg gagcggcgcc gcttcttccg gtgcacctgt
     1441 ctggatcgaa tcttctcgcg aagaaaaact cgcatccgcc gcctcctacg tccgctggct
     1501 gatcgcacgc gacagcaaaa tcacgccgct caaaactctg caaggcaaaa tctgggaaca
     1561 aggttttcgc agcggcgccc taaaaggcga agtctatccc gacgtagctc cagccttcgc
     1621 ccgttggcgc acacaacata aacgcatcgt cactttttct tccggcagcg tcctcgccca
     1681 acaacttctc ttcggccact ccaccctcgg tgacctctcg tcattcatcg aagcctattt
     1741 cgacaccatc accgggccca agcgcgagcc cgccagctat cgcaaaatcg ccgctgccct
     1801 gaaactctct cccaccgcgg ttctctttgt ctctgacaca ggccaagagc tggatgccgc
     1861 gcgcgatgcc gacctgcgta cagctctctc attgcgggcc ggcaacccca gcgaaattac
     1921 cgtggtcgaa caacggcatc ccgcgatcca caactttaat gaactctttg ctgcttaggt
     1981 gcagggcgga ctttactcgc cgggccgcga gatgactggc cacctgccct gagactgtcc
     2041 cttcggccaa ttggcgctga cttcccagac ccttatcttg cacgcaaccc cggccggcaa
     2101 tcccgtgcag ctcaatcaag ctcatggagg t
//
LOCUS       DASTPM010000281         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_211854, whole genome shotgun sequence.
ACCESSION   DASTPM010000281 DASTPM010000000
VERSION     DASTPM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211854"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /gene="purH"
                     /locus_tag="VFR42_04975"
     CDS             complement(<1..257)
                     /gene="purH"
                     /locus_tag="VFR42_04975"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0368536.1"
                     /translation="MAVERALISVFDKTGVLDFAKRLAALKIEILSTGGTAKLLRDSG
                     VAVKDVSEFTGWPEMLGGRVKTLHPKVHGGLLFRRNLAEDQ"
     gene            complement(386..1003)
                     /locus_tag="VFR42_04980"
     CDS             complement(386..1003)
                     /locus_tag="VFR42_04980"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEU0368537.1"
                     /translation="MATIDEELGAMERDIRQLKIEYDQYFGGGRKRPPNEIEWRIDLI
                     VKKYGERSGDMKIGQRFRFNNLTQTYAKYKDIFRKKLRQREEGTVQRHFGAAAKAIEA
                     ERTRAAEAAQAQAAAAVAELPGAFRIVCSHPEKETEKVEQLFRAFIEAKKSAGEDVGR
                     VSHAGFSEFVRKKTKDLQKQNACNDVEYVVEMVGGQVKLKALVKA"
     gene            1183..2610
                     /gene="selA"
                     /locus_tag="VFR42_04985"
     CDS             1183..2610
                     /gene="selA"
                     /locus_tag="VFR42_04985"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU0368538.1"
                     /translation="MESSTKRSLGSDKAALLRQIPSVDELLHQPRLAKLAARIPRELL
                     VEVAREVLAGVRDEITGERGVAVIPVIPANLEEVIAREVERILARTLQPVINATGVIL
                     HTNLGRAPLAPAVLEELRQTAIHYSNLEYDLDAGARGKRDVHTSELIARLTGAEDAIV
                     VNNCAAAILVVLAALAKGGEVLVSRGELIEIGDGFRIPEVMAQSGAVLREVGTTNRTH
                     VEDYESAISDKTRLLLRVHPSNFQMSGFTEKPSIEDLVELSRRANLPLIEDLGSGCLV
                     DLSPYGIQEPTVRQSVEAGISLVTFSGDKLLGGPQAGIIAGKKELVQRVRRHPLFRAL
                     RVDKLIIAALEVTLGSYQRGALDEIPAMAMIRATPREIEERSRHFIRELTRSIPMDRL
                     DIEITNGESLAGGGSTPAQSLRTKIIRIRSDRLSASQIEQRLRRGAATSVIARVEDNS
                     VVLDLRTVFAEQETALAEALTAALR"
     gene            2680..2952
                     /locus_tag="VFR42_04990"
     CDS             2680..2952
                     /locus_tag="VFR42_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368539.1"
                     /translation="MAAALIHFDPKQKRLLTRRAKERGRSFSQEVRNAVDLYLSVPVE
                     TEEGLSLLAKAANEAADRMIERLDETIAHVDRTLKQYRKVHNELGR"
     gene            2936..3145
                     /locus_tag="VFR42_04995"
     CDS             2936..3145
                     /locus_tag="VFR42_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368540.1"
                     /translation="MSWAASAYAAIRKIVLLEDRMESLSTQVVGLATTFSELDRRLIR
                     LEAKFELLERMASPSRRALPEKSEK"
BASE COUNT          639 a         1014 c          839 g          662 t
ORIGIN      
        1 ttctgatcct cggcgaggtt gcggcgaaac agcagcccgc catgcacctt ggggtgcagc
       61 gtctttacgc gcccgcccag catctccggc cagccggtaa actccgaaac atccttcact
      121 gccacaccgg aatcgcgcag caactttgct gtgccgcctg ttgagagaat ttcaatcttc
      181 agcgccgcca gccgcttggc gaagtccaac acacctgtct tgtcgaagac gctgatcagc
      241 gcgcgttcca ccgccatgtc gttccctcca ctgtaggaaa acacttcagt gtaagcggaa
      301 cgcgcagaaa tggaaaagca gcacgctgtg ggaaactttg aagactctcg agggaacaat
      361 gaagtaaatc aggtcaagaa cgaggttaag ctttgaccag cgccttgagc ttcacctggc
      421 cgccgaccat ttcgaccaca tactccacgt cgttgcaggc gttttgtttc tgcaaatctt
      481 tggtcttctt gcgcacaaat tccgagaagc ccgcgtggga aacccggccg acatcctctc
      541 ccgccgattt tttcgcttcg ataaaggcgc gaaatagctg ctccactttt tccgtttctt
      601 tttcgggatg cgaacaaacg atgcgaaacg ctccgggcag ctctgccacg gctgcggccg
      661 cctgcgcctg tgcggcttcg gctgcacgcg tgcgctccgc ctcgatcgct ttagccgctg
      721 ccccgaaatg tcgctgcacg gttccctctt cgcgctgccg cagcttcttg cggaaaatgt
      781 ccttatattt cgcgtaggtc tgcgtgaggt tgttgaagcg aaagcgttgg ccgatcttca
      841 tgtcgcccga acgctcgcca tacttcttga cgatcagatc gatccgccac tcaatttcgt
      901 tgggcgggcg cttacgccct ccgccgaaat attgatcgta ttcgatcttc agctggcgga
      961 tgtcgcgttc cattgcgccg agttcttcgt caatcgtagc catgggacaa tgctcgctgc
     1021 ctatcctatg gggcgcgcgc cggtgcgtca atgaatcggt agtcctggga aactcatggt
     1081 gaactgtacc agccccggct tcacctgtac caaagtacag gcactgttcg tcaaagcgcg
     1141 gccagcattg ctccctttcg gcgcctgtgc tccaatagcc tcatggagtc cagtacgaaa
     1201 cgctctctcg gctccgacaa agccgctctg ctgcgccaga ttccttcggt ggacgaactc
     1261 ctgcatcagc cgcggttggc caaactcgcg gctcgcatcc ctcgcgaact gctcgttgaa
     1321 gtcgcccgcg aagtcctggc gggcgtgcgc gacgaaatta ccggtgagcg tggtgtcgcg
     1381 gtcattccgg ttatccccgc caatctggaa gaggtgatcg cccgcgaagt cgaacgcatc
     1441 ctggcgcgca cgctgcaacc ggtcatcaat gccaccggtg tgatcctgca caccaacctt
     1501 ggccgcgccc cactcgcccc ggccgtactc gaagaacttc ggcaaaccgc cattcactat
     1561 tcgaatctcg agtacgacct ggacgccggt gcacgcggca aacgcgacgt tcacacttcg
     1621 gagctgattg cccgcctcac cggcgccgag gacgccatcg tcgtcaacaa ctgtgctgct
     1681 gcgattctgg tagtcctcgc agcactcgcc aagggcggcg aagtgctcgt ctcgcgcggc
     1741 gaactcatcg aaattggcga tggctttcgc atcccggaag taatggcgca aagcggcgcc
     1801 gtcttgcgtg aggtcggcac caccaatcgc actcatgtcg aagactacga gagcgcgatc
     1861 agtgacaaga cgcgcttgct gctgcgcgtc cacccatcca atttccagat gtccggattc
     1921 accgagaaac catctataga agacttggtc gaactcagtc ggcgcgccaa tcttccttta
     1981 atcgaagacc tcggctccgg ctgcctggtg gacctttctc cttacggcat tcaggagccg
     2041 accgtccgcc agagtgttga ggctggtatt tcgctggtaa ccttcagcgg ggataaattg
     2101 ctcggcggcc cccaggccgg catcattgcc ggcaaaaagg aactggtgca gcgggtccgc
     2161 cgccatccgc tctttcgcgc tctgcgtgta gataagctca tcatcgccgc gctcgaagtt
     2221 accctcggtt cctaccagcg cggcgccctc gatgaaatac ctgccatggc catgattcgc
     2281 gccacaccgc gcgagatcga agaacgcagc cgccacttca ttcgtgaact gacacgctcc
     2341 atcccgatgg accgcctgga catcgaaata accaacggcg aatccctggc cggcggcggt
     2401 tctactccgg cgcagtctct tcgcaccaaa atcattcgca tccgcagtga ccgcctctct
     2461 gcttctcaaa tcgagcaacg cctgcgccgc ggagctgcga cttccgtcat cgctcgagtc
     2521 gaagacaatt cagtggtgct ggatctgcgc acggtgtttg ccgaacagga aactgccctc
     2581 gccgaagccc tcacggccgc tttgagataa attggcgcgc ctcactggtt tctcacagcc
     2641 tcgatcatgc tatgatataa ctctgatata ggagggtcga tggctgccgc tctaatccat
     2701 tttgatccca aacaaaagcg cttgctgacc cgtcgcgcca aggagcgcgg gcgatccttt
     2761 tcccaggaag tacgcaatgc cgtggacctc tatctttccg tgccagtcga aaccgaggaa
     2821 ggactctccc tcctggcgaa agccgcgaac gaggcggccg accgcatgat cgaacggctc
     2881 gacgaaacga tagctcacgt cgatcggaca ctgaagcaat accgcaaggt ccacaatgag
     2941 ctgggccgct agcgcctacg cagcgatccg caagatcgtc ttgcttgagg accgcatgga
     3001 gagcctcagc acccaagtcg ttggccttgc cacaactttc agcgaactcg accgccgact
     3061 gattcggctc gaagcaaagt ttgaactgct cgagcgaatg gcgtctccct cgcggcgcgc
     3121 gcttccggaa aaatccgaaa agtagattcc cttg
//
LOCUS       DASTPM010000282         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_211877, whole genome shotgun sequence.
ACCESSION   DASTPM010000282 DASTPM010000000
VERSION     DASTPM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211877"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..2350
                     /gene="fusA"
                     /locus_tag="VFR42_05000"
     CDS             89..2350
                     /gene="fusA"
                     /locus_tag="VFR42_05000"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0368541.1"
                     /translation="MGRAFKLEDTRNIGIMAHIDAGKTTSTERILFYTGVTYKIGSVD
                     EGTATMDWMEQERERGITITSAATTASWTRFDKKYRVNIIDTPGHVDFTVEVERSLRV
                     LDGAVCVFDSVAGVQPQSETVWRQADKYRVPRICFVNKMDRMGADFDHVVRTIRQRLG
                     AHPVPLQFPLGREDNFIGIIDFLEQKVVVWLEETLGAKYEIFEVEKLWDKAFVDSRPD
                     VAAALKGSAIDKAFYDEHRNKVVEYIAEHDDEVLDKYLTEGTLSLEEMRKSIRKSTLA
                     LKIVPVLAGSAFKNKGIQPLLDAVIDFLPSPIDVPPVEGVNPEDDETVLRRAADDQPF
                     AALAFKIMSDPFVGHVTYIRVYSGVLKSGSYVYNSGKRSRERISRLLQMHANKREEID
                     EVYAGDICAVVGLKSVNTGDTLCDENKQVILESIEFPAPVISVAIEPKTKADQDRLGV
                     ALQRLAQEDPTFRVSTEPDTGQTLISGMGELHLEIIVDRMLREYNVQANVGRPQVAYR
                     ETIRRKAEAEGKYIKQTGGKGQYGHAVIELHPLPSSQHENMAEMSTDDLDALAKQVTA
                     SSNPGKWKFDKEHRFLFIDKIVGGSIPREFIPPIEAGIREALDTGILAGFPTVDVAVV
                     LTDGSYHDVDSNEMAFKIAGSMAYKEACAKAKPVLLEPIMKVEVVVPEEYMAAVFGDL
                     NSRRSAIQGTEYRAGSNVIRVEVPLAQMFGYATDLRSRTQGRATFTMHFSHYAELPSN
                     LAEEVVKKARGEK"
     gene            2443..>2827
                     /locus_tag="VFR42_05005"
     CDS             2443..>2827
                     /locus_tag="VFR42_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0368542.1"
                     /translation="MAKEKFERSKPHVNIGTIGHIDHGKTTLTAAITKVLSKHNPKVQ
                     FRAFDSIDNAPEERERGITIAVSHVEYETPNRHYAHMDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVG"
BASE COUNT          657 a          696 c          919 g          555 t
ORIGIN      
        1 aagatggcgg cagtacaaca aaaggatttg gtgagctgtg agtgaagcag tagcaacgcg
       61 gggagaaaaa agggccgggc aaccgactat gggcagggca ttcaaactcg aagacacgcg
      121 caatatcggg atcatggcgc atattgacgc ggggaagact acgtctaccg agcgcatcct
      181 gttctatacg ggtgtgacct acaagatcgg ttcggtggat gagggcacgg ccaccatgga
      241 ctggatggag caggagcgcg agcgcggaat tacgattacc tcggcggcga caactgcttc
      301 gtggacgcgc ttcgacaaga agtatcgcgt caatattatt gatacgcctg gccacgtgga
      361 ctttacggta gaggtagagc gttcgctgcg ggtgctggac ggcgcggttt gcgttttcga
      421 cagcgtggcg ggcgtgcagc cacagtcgga aacggtgtgg cgccaggccg acaagtatcg
      481 cgttcctcgc atttgtttcg tcaacaagat ggaccgcatg ggcgcggatt ttgaccacgt
      541 cgtgcgaacg attcgccaac gcctcggtgc ccaccccgtt cctctgcagt ttcccctcgg
      601 tcgcgaagat aatttcatcg ggattattga tttcctcgaa caaaaagttg tggtgtggct
      661 ggaagagacg ctgggcgcga aatacgaaat ttttgaagta gaaaaactgt gggacaaagc
      721 gtttgtggat tcgcggccgg atgtggccgc agcgctgaag ggttccgcga tcgacaaggc
      781 gttttacgac gaacaccgca acaaagtggt ggagtacata gccgagcacg acgacgaagt
      841 gctggacaag tatttgacgg aaggcacgtt gtcgctggaa gagatgcgca agtcgatccg
      901 caaatcgacg ctggcgctga aaattgtgcc ggtactggcg ggttcagcgt tcaagaacaa
      961 aggcattcag ccgctgctgg atgcagtgat tgatttcttg ccgtcgccga tcgatgtgcc
     1021 gccggtggaa ggcgtgaatc cagaggacga cgaaacggtt ttgcggcgcg ctgcggatga
     1081 tcagccgttt gccgcgctgg cattcaagat catgtccgac ccgttcgtcg ggcatgtgac
     1141 gtacatccgc gtgtattcgg gcgtactgaa atcgggcagc tatgtgtaca actccggcaa
     1201 gcgttcgcgc gagcgcatca gccgcttgct gcagatgcat gccaacaagc gcgaagaaat
     1261 tgacgaagtg tatgccggcg acatttgcgc ggtggtcggg ctgaagagcg tgaacaccgg
     1321 cgatacgctg tgcgatgaaa acaagcaggt gattctggaa tcgattgagt ttccagcgcc
     1381 ggtaatttcg gtggcgattg aaccgaagac caaagcggac caggatcgct tgggggtggc
     1441 gctgcagcgt ttggcgcagg aagatccgac attccgtgtt tcgaccgagc ccgataccgg
     1501 ccaaacattg atttccggaa tgggcgaact tcatctggaa atcatcgtgg accgcatgct
     1561 gcgcgagtac aacgtgcagg caaacgtggg ccggccgcag gtggcgtacc gcgaaacgat
     1621 tcgccggaag gccgaagcgg aaggcaagta catcaagcag accggcggta aaggtcagta
     1681 cggccatgcg gtcattgagc tgcatccgct gccgagttcg cagcacgaga acatggcgga
     1741 aatgtcgacc gacgatctgg atgcgctggc caagcaggtc acggcaagca gcaatccggg
     1801 caaatggaaa ttcgacaagg aacaccggtt tctgttcatt gacaagattg tgggcggatc
     1861 gattccgcgt gagtttattc ctccgatcga ggcgggcatt cgcgaggctt tggacaccgg
     1921 cattttggcg ggttttccga ctgtggatgt ggcggtggtg ctgaccgacg gcagctacca
     1981 cgatgtggac tcgaacgaaa tggcgttcaa gattgccggt tcgatggctt acaaggaagc
     2041 ttgcgcgaaa gccaagccgg ttttgctgga accgatcatg aaagtggaag tagtagttcc
     2101 ggaagaatac atggcggcgg tgtttggcga tctgaactcg cggcgttccg cgattcaggg
     2161 tacggaatac cgcgcgggca gcaacgtgat tcgcgtggaa gtgccgctgg cgcagatgtt
     2221 cgggtacgcc acggatttgc gcagccgcac gcagggtcgc gcgacgttca ccatgcactt
     2281 ttcgcactac gcggaattgc cgagcaatct ggcggaagaa gtcgtcaaga aagcgcgcgg
     2341 cgaaaagtaa aggatgtagc ggtagaagtt tgatttgcac gaattcgatc agtgtttcag
     2401 agcttgtagt tgcgagcttt gcgacaacga ggagcagagg gaatggccaa ggagaaattc
     2461 gagcgcagta agccgcacgt gaacattggg acgattggtc acattgacca tggcaagacg
     2521 acattgacgg cggcgatcac caaggtgctc tcgaagcaca atcccaaggt gcagtttcgg
     2581 gccttcgatt cgattgacaa tgctccggaa gagcgcgagc gcggtatcac cattgcggtt
     2641 tcgcacgtgg agtatgaaac gccgaaccgg cactacgcgc acatggactg cccgggacac
     2701 gccgactaca tcaagaacat gatcaccggc gcggcgcaga tggatggcgc gattctggtg
     2761 gttgccgcga ccgacggacc gatgccgcag acgcgcgagc acattctgct ggctcgccag
     2821 gtcggcg
//
LOCUS       DASTPM010000283         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1105732, whole genome shotgun sequence.
ACCESSION   DASTPM010000283 DASTPM010000000
VERSION     DASTPM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105732"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..824)
                     /locus_tag="VFR42_05010"
     CDS             complement(144..824)
                     /locus_tag="VFR42_05010"
                     /inference="COORDINATES: protein motif:HMM:NF020922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HEU0368543.1"
                     /translation="MAAGKPIFTAEIFRFFRDLKKHNKKAWMDANRERYQETVCKPFR
                     RLLEELSPALLKLDANFDVAARGGRNFSRINRDIRFAKDKTPYRTQMYVKCSVPLPGE
                     MESGEMYLGISADTVTCGFRIYAVPKRKHSPIARVAEPKLAANPRLLARQKKRLAKKY
                     DSYWYSTVKGEWTKNDGWPTLEDWGKLQAWIVRRKLRKSDATRASLPADAARIFREVY
                     PLLEFTSI"
     gene            933..>2339
                     /locus_tag="VFR42_05015"
     CDS             933..>2339
                     /locus_tag="VFR42_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368544.1"
                     /translation="MRRSALILSLFFVVSSANLSWADEKAGTSDKKEPWKPEDIVYGE
                     TAAPQTRISPDGKWLVWVKSNGDKEKDARVSNLILSSLTESKEIQLTRGGDNNGQPQW
                     SPDGQLIAFTSNRARQGAKPDNAPVQIWIINPFGGEPWSITELARAPRRIDWLDKDTV
                     VFSAQEDPSLYAQEMKQKKDDSEVVDDSEHEPPVRLFKINVKDNKITRLTANTDWIGN
                     WAASKDGKFVAAVHEKSLHYTFDQKTPPIAVLHNLSDGTEKRIFAEGRIYPRGFEWLP
                     DNLGFYAWAPYSTHPKFLTATVELLYFYDVAGGKSVQVPLDWENGIGSDLAATQDGFV
                     VGLAAGARFETSRYTRSKAGDGWAWKRATLEGEHAKNIQFFAVTEEAKEIVYFYSTAS
                     KLPQIYRARLDGTQLVSPVQVSKLNEGLVKNRAFAKTEVVRWKGANDQEVEGILYYPA
                     NYEAGKKYPLITAIHGGPS"
BASE COUNT          517 a          639 c          684 g          499 t
ORIGIN      
        1 ttcgtcccac gaagatcatc ctgaacggca cagcacgtcg ctgatgctca cagcatgaag
       61 gacaccggcc gaagcgtctg tggccgctac cttcccgagc gcagctgttt gcccgaacga
      121 ttccctattg ctcgaagagt tcgctatatg gaggtaaatt cgagtagcgg gtagacctca
      181 cggaagattc tcgccgcgtc ggcgggcaga gacgcgcgcg ttgcgtccga cttgcgtaac
      241 tttctgcgga cgatccaggc ttgcagtttc ccccaatcct ctaaggtggg ccagccgtcg
      301 tttttcgtcc attcgccttt cacagtggaa taccagtagc tatcgtactt tttcgcaagc
      361 cgtttctttt gccgagccag caagcgcgga ttggcagcca gcttcggctc ggctacccgt
      421 gcaattggcg aatgtttgcg tttcggcact gcgtaaatgc ggaagccaca ggtcaccgtg
      481 tccgcggaaa ttccaagata catttcaccg ctttccattt cgccgggcag cggcacgctg
      541 catttcacgt acatctgcgt gcgataaggc gtcttgtcct ttgcaaagcg gatgtcgcgg
      601 ttgatgcgcg aaaaattcct tccgccgcgc gccgccacgt cgaaattcgc gtccagcttc
      661 agcagcgccg gcgacaattc ctccaataac cgccggaacg gtttgcacac ggtttcctga
      721 taccgctcgc ggttcgcgtc catccacgct tttttgttgt gcttcttcaa atctcggaaa
      781 aaccggaaaa tctccgccgt aaagatcggc ttccctgccg ccattgcgtc tctccttgcc
      841 aacgagcatt tcagcgtacc tcattcccaa ttcccgtcaa ttcgactatc cttttccgtt
      901 cgccaaacat ctctcgaatc caaggatcct cgatgcgccg ctctgctttg attctttccc
      961 tgttcttcgt tgtgtcttcc gcaaatcttt cgtgggcaga cgaaaaagct ggtaccagcg
     1021 acaagaagga accgtggaag ccggaagaca tcgtctatgg cgaaacggcg gcgccgcaga
     1081 cgcgcatctc cccggacggc aagtggctcg tctgggtgaa gtccaacggc gacaaagaga
     1141 aggacgcgcg cgtttcgaac ctgatactct ccagcctcac cgaaagcaag gaaattcaac
     1201 tgacgcgggg cggcgacaac aacggccagc cgcagtggtc ccccgacggc caactgattg
     1261 ccttcaccag caatcgcgca cggcagggcg cgaagcccga caacgcgcca gtgcagattt
     1321 ggatcatcaa tccatttggt ggcgagccgt ggtcgattac ggaattggcg cgcgcgccgc
     1381 gtcgcatcga ttggctggat aaagatacgg tcgttttcag cgcgcaggaa gatccgtcgc
     1441 tctacgcgca ggaaatgaaa cagaagaaag atgattcgga agttgtggat gactcggaac
     1501 atgaaccgcc ggtgcgcttg ttcaagataa atgtaaagga caacaagatc acacgtctga
     1561 ctgcgaacac agactggatt ggcaattggg cggcgtcgaa agatggaaaa ttcgtcgcgg
     1621 cggtgcacga aaaaagcttg cactacacgt ttgaccagaa gactccaccg attgcggtgt
     1681 tgcacaactt aagcgatgga acggagaagc ggattttcgc ggagggccgc atctacccgc
     1741 gcggatttga gtggttgccg gacaatttgg ggttttatgc gtgggcgccg tactccaccc
     1801 atccgaaatt tctgacggcg acggtggagc tgctttattt ttacgacgtg gcgggcggga
     1861 agagtgtaca ggtgccgctg gattgggaga acggcatcgg gtcggacctg gcggcgacgc
     1921 aggatggatt tgtggtggga cttgcggcgg gggcgcgatt tgagacttcg cgttacacgc
     1981 gcagcaaggc gggtgatgga tgggcttgga agcgcgcgac gctggaaggc gagcacgcga
     2041 aaaacattca gttcttcgcg gtgacggaag aagcgaagga aattgtttat ttctattcga
     2101 cggccagcaa gctgccgcag atttatcgcg cacggctcga cggtacgcag ctggtttcgc
     2161 cggtgcaggt gagcaaactg aacgaagggc tggtgaagaa ccgcgctttc gcaaagactg
     2221 aagttgttcg ctggaagggc gcgaacgacc aagaagtgga agggattctc tactacccgg
     2281 cgaattacga agcggggaaa aagtatccgc tgattacggc gattcatggc gggccgagc
//
LOCUS       DASTPM010000284         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_990294, whole genome shotgun sequence.
ACCESSION   DASTPM010000284 DASTPM010000000
VERSION     DASTPM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990294"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFR42_05020"
     CDS             complement(<1..656)
                     /locus_tag="VFR42_05020"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEU0368545.1"
                     /translation="MSRIHEALKRAEQERAASQGTRTDSSPVTDLPASDSGPVMPDLT
                     HTAAAAAASAPAPAGPGNKASAPSFTNAFTFDTLLARCSPAEWQPDAKTMLFFNQEEN
                     ALGTEQFRTLRSRLYQMREKVSLKKILVTSALPQEGKSFVSANLAQVLVRQHGRRVLL
                     IDGDMRVARLHTALGTTSSPGLADYLLGESDEFSVMQRGPMENLFFIPCGRTVAHPAE
                     L"
     gene            complement(653..1621)
                     /locus_tag="VFR42_05025"
     CDS             complement(653..1621)
                     /locus_tag="VFR42_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0368546.1"
                     /translation="MYKEFFGLRANPFNVNPDPRYLFLTRHTEEALACLTYGIQSRKG
                     FVLLTGEVGTGKTTLINKLLEWLRLQQVATAFIFNSRMNVPQFLDYMMADFGIPCDSK
                     SKSQILQRLYNWLLDRYRAGETAVLIVDEAQNLTDEVLEEIRMLTNLETFTEKLLQIV
                     LVGQPELETKLKQPQLRQLRQRLTLRAKTHPLTLEETKAYVQQRLRIAGSDGRQIFDG
                     EALNAVHKYSNGIPRVVNLLCEHCLVSAFVDQKPTIHEGVVDAVARDFDLSDNIASGA
                     MTVPAAPPAGTPGAERFDLVEALRTLATLADRLRQSEQDLPKERKS"
     gene            complement(1631..3187)
                     /locus_tag="VFR42_05030"
     CDS             complement(1631..3187)
                     /locus_tag="VFR42_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEU0368547.1"
                     /translation="MIENRDLTLDDYLAMLRRRSKMILIPALLAPLVGFLISYAFTAK
                     YTSQSLVLVEGQKVPEGVVQPVVTADLAQRIATMQQQVMGRNRLQPIVEKRPNLFKGG
                     KSLEDVLEEVRAGVQIEPVVTDLSQIANTGGGKRKSGQGSVPGFYVNFTTATPRDAQD
                     ICNDLTSAMLTEFQNSREQVAVSTTEFISRQLDEAKRNLDEQDAKLAAFKRQYAGQLP
                     GDEDNNLKVLGALNSQLDANTQTVNRAQQDKSYTESMLAQQLAAWKSAQGSNNPQSLE
                     TQLTQLQSQLLSLQARYTEDHPDVIKTKADIAEVKKRLAEVNEAAAKGGDANVKASAG
                     EPPEIRQLRLQVHQYENVLANATREQKRIGDQIRMYQGRVAISPDVEEKYKLLTRDYD
                     TAQKFYADLLAKKSTSEMATDMERRQQGEQMHLLNQANLPESPSFPNRLLFAAGGLGG
                     GLVIGMGLALWLELRDKSIRNEADVVASLQMPVLVSLPWVTEEAPSKNGKFWNRDKAD
                     AEAVKEKVEV"
     gene            complement(3228..>4097)
                     /locus_tag="VFR42_05035"
     CDS             complement(3228..>4097)
                     /locus_tag="VFR42_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368548.1"
                     /translation="MGILGSGFGGHFGIMGSQQLGSLGQEPRLTNVVLADVVQAISPR
                     SSLTAAGNFGVVDFIDNTQGLINSRQTGGQVGYSHSISRKDQFALTYGYQRFRFPAEV
                     GADDVNTHVVQAMYGHRISGRMDLVIGLGPQWTHVNDPLTGESSRLSAAGRFTLRYQF
                     RQTATSLAFERYDTSGSGFFAGAISNIARAQASRRLGRRYTGQVALGFAHNQRLQQST
                     LGVAASTYNYGFAGLALRRQFGYNWGAFLAYQWNDQIFNTCPVVGTTCNRISVRHVLT
                     LGVDWHFRPIRLD"
BASE COUNT          774 a         1292 c         1135 g          896 t
ORIGIN      
        1 agttctgccg gatgagccac ggtccgcccg cagggaatga agaacaggtt ttccatcggc
       61 ccgcgctgca tcaccgaaaa ctcatcgctc tcacccagca ggtaatccgc aagaccgggt
      121 gaagaggtgg tacccagggc ggtgtgcagg cgtgccacac gcatatcgcc gtcgatcaag
      181 agaacgcgcc gcccatgctg ccgcaccagc acctgcgcca ggttggccga gacgaacgac
      241 ttgccttcct gcggcaatgc actggtcacg agaatcttct tgagtgacac cttttcgcgc
      301 atttggtaga gccgtgaacg cagcgtgcga aactgctccg tacccaacgc gttctcttcc
      361 tggttaaaga agagcatggt cttggcatcc ggctgccact ccgccgggct acagcgcgcc
      421 agcaaggtgt cgaacgtgaa agcgttggtg aacgaaggcg ccgaggcctt gttgcccgga
      481 ccagcgggcg caggcgccga agccgcagct gccgccgccg tatgcgtcaa gtccggcatc
      541 accggtccag agtcggacgc tggcagatcc gtcacgggag atgagtccgt gcgggttccc
      601 tgcgacgctg ccctttcctg ttcggctcgt ttcagtgctt cgtggattcg gctcatgatt
      661 tcctctcttt gggcagatct tgctcacttt ggcgcagccg gtctgccagg gttgccagcg
      721 tacgcagtgc ttccaccaga tcaaatcgtt ccgcgcccgg agtgcctgcg ggcggtgctg
      781 cgggaaccgt catcgccccc gacgcgatgt tgtcgctgag gtcaaaatca cgcgctaccg
      841 cgtccaccac gccctcgtga atggtgggct tctggtcgac aaaggcgctt accaggcagt
      901 gttcacagag caggttcacc acgcgcggaa ttccattcga atacttgtgc accgcgttca
      961 acgcttcgcc gtcgaaaatc tgccgcccgt cggaacccgc gatgcgcagg cgctgctgca
     1021 catacgcttt cgtttcctcc agggtcagcg gatgcgtctt ggcccgcagc gtgagccgtt
     1081 gccggagttg ccgcaactgc ggctgcttca acttcgtctc cagttctggc tgtcccacca
     1141 ggacaatctg cagcaacttc tcggtgaagg tctcgagatt cgtcagcatg cgaatctctt
     1201 ccagcacctc gtcggtaagg ttctgcgctt catcgacaat cagcaccgca gtctcgccgg
     1261 cccgataacg atccagcagc cagttataga ggcgctgcag aatctggctc ttcgacttcg
     1321 aatcgcacgg aatcccgaag tccgccatca tgtaatcgag gaattgcgga acattcatgc
     1381 gcgagttgaa gatgaaagcc gtcgccacct gctgcaggcg caaccactcc agcaacttgt
     1441 tgatcaacgt cgtcttgcct gttccgacct caccggtcag aaggacaaag cctttgcggc
     1501 tctgaatgcc gtaagtcagg cacgcgagcg cctcttccgt gtgccgcgtc agaaacagat
     1561 aacgcggatc cggattgacg ttgaacggat tggctcgtag tccaaaaaac tctttataca
     1621 taccgttcgc ctagacttcg actttctcct tgacggcttc cgcatctgcc ttgtcgcggt
     1681 tccagaattt tccgttcttg ctcggcgcct cttccgttac ccagggcagc gatacgagca
     1741 ccggcatctg caaactggcc acgacatccg cttcgttgcg aatcgacttg tctctcaact
     1801 ccagccacaa cgcgaggccc atcccaatca ccaatccgcc acctaaccca ccagcggcaa
     1861 acaacagccg gttcggaaag ctgggcgatt ccggcagatt ggcttggttc agcaggtgca
     1921 tctgctcgcc ctgctggcgc cgttccatgt ccgtggccat ttccgacgtg ctcttcttgg
     1981 ccagcaggtc cgcatagaac ttctgcgccg tgtcgtaatc gcgggtcagc agcttgtatt
     2041 tctcttccac atccggactg atcgccacgc ggccttgata catacgaatc tgatcgccga
     2101 tgcgcttttg ctcgcgggtc gcgttcgcca gaacattctc gtattggtgg acctgcaagc
     2161 gaagttggcg aatctccggc ggttccccgg cgctggcctt gacattggcg tctccgccct
     2221 tggcggcagc ctcgttcact tccgcgagtc tcttcttcac ctcggcaata tcggccttgg
     2281 ttttgataac gtccggatgg tcttcggtat atcgagcctg caatgacagc agttgactct
     2341 gaagttgggt cagctgggtt tccaggctct gcggattgtt cgacccctgc gccgacttcc
     2401 acgctgccaa ctgctgtgcg agcatggatt cggtgtacga cttgtcctgc tgcgcgcgat
     2461 ttacggtttg cgtgttcgca tccagctgcg aattcagcgc acccagcacc ttgaggttat
     2521 tgtcttcatc gccaggaagc tgcccggcgt actggcgctt gaatgcagcc agcttggcgt
     2581 cctgttcatc caggttccgt ttggcttcat ccagctgccg gctgatgaat tcggtggtgc
     2641 tgaccgccac ctgctcgcgc gaattctgaa attccgtcag catcgccgag gtcaggtcgt
     2701 tgcaaatgtc ttgcgcgtca cgcggggttg ccgtcgtgaa gttcacgtag aaacccggca
     2761 cgcttccctg cccactcttt cgtttaccgc cgccggtatt ggctatttgg ctgaggtcgg
     2821 tgaccaccgg ctcaatctgc acgcctgccc ggacttcctc aagaacatcc tccagacttt
     2881 ttccgccctt gaacaggttg ggccgcttct cgacaatcgg ctgcaaccga ttccggccca
     2941 tcacctgctg ctgcatggtg gcaatgcgct gtgccaggtc ggcggtgacc accggctgca
     3001 ccacaccctc gggcactttc tgtccttcca ccagcacgag ggattgcgat gtgtacttcg
     3061 cagtgaaggc gtaggaaatg aggaaaccga ccagcggcgc taacagcgcc gggatcagaa
     3121 tcatcttcga gcgccggcga agcatcgcca gatagtcatc cagagtgaga tcgcgatttt
     3181 caatcattcc agtgtttccg ttctggctgc attgtcagca gcctgtatta atcgagtcga
     3241 atcggccgga aatgccaatc cacgccaagt gtcaggacat gtcttactga aattcggtta
     3301 caggtcgtgc ctacgaccgg gcacgtgttg aagatctgat cgttccactg atacgcgagg
     3361 aaggcgcccc agttgtatcc aaactgccgg cgcagggcga ggccggcgaa accgtagttg
     3421 taggtgctgg ctgccacccc gagcgtactc tgctgcaggc gctggttatg cgcgaagccc
     3481 agagctacct ggcccgtata ccgccgcccc agtctccggc ttgcctgcgc ccgcgcgata
     3541 ttactgatgg cacccgcgaa gaaacccgaa ccgctagtgt cataacgttc gaacgccagc
     3601 gaggtcgccg tttgacgaaa ttggtagcgc agcgtaaatc ggccggcggc cgaaagccgc
     3661 gagctttccc cagtgagggg gtcgttcacg tgcgtccact gcggacccag accgatcacc
     3721 agatccatgc gtccagaaat tcgatgcccg tacatcgcct gcaccacgtg tgtgttcaca
     3781 tcgtcggcgc ccacctcggc aggaaaacga aacctctggt aaccgtaggt cagcgcgaac
     3841 tgatccttcc ggctaataga gtggctgtag cctacctgtc ccccggtttg ccggctgttg
     3901 ataagtcctt gcgtgttatc gataaagtcc accacgccga aattgccagc cgctgtcagc
     3961 gacgagcgcg gactgatggc ctgcactacg tccgctagca ccacattggt gaggcgaggc
     4021 tcttggccaa gcgaccctaa ctgctgcgag cccatgatcc cgaagtggcc gccaaagcct
     4081 gacccgagaa tgcccat
//
LOCUS       DASTPM010000285         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_298201, whole genome shotgun sequence.
ACCESSION   DASTPM010000285 DASTPM010000000
VERSION     DASTPM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298201"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..546
                     /locus_tag="VFR42_05040"
     CDS             253..546
                     /locus_tag="VFR42_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0368549.1"
                     /translation="MAAKPLSKTKIVAHIAEKLGTPKKQVATFFEELHKLAVKEAKGG
                     AGKFVIPGIGRVVKAHRKARMGRNPQTGEAIKIKAKTVVRLRPAKALKDAILK"
     gene            complement(731..1108)
                     /locus_tag="VFR42_05045"
     CDS             complement(731..1108)
                     /locus_tag="VFR42_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0368550.1"
                     /translation="MNRILAVLGIAALGTSLAVAGVGLAQTKTTKKSPAGKTDAAAAA
                     RGKELFQQKCSTCHYDTSDAKKIGPGLKGLNKRGTFTVNNNKVTDDNLKTWIENGDSL
                     MPPFKDVIDDSQIKDVIAYVKTL"
     gene            1318..1800
                     /locus_tag="VFR42_05050"
     CDS             1318..1800
                     /locus_tag="VFR42_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0368551.1"
                     /translation="MNPETLTQAWEVLQDAYQAQMEGDYERAVELYQSSLDLHPTAEA
                     HTFLGWTYHFQGRLEDAIAECKRAIEIDPDFGNPYNDIGAYMIEQGNLDQAIPWLQQA
                     IDSKRYEPRHFPHYNLGRAYLGKEMYSQAMRCFQEALEIEPRYGLARQALDSLRRMLN
                     "
     gene            complement(1797..3074)
                     /locus_tag="VFR42_05055"
     CDS             complement(1797..3074)
                     /locus_tag="VFR42_05055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368552.1"
                     /translation="MSFQQRLQDVRTGFERPFWVANISEIFERLAYYGAFASLANYLR
                     ETLSFPTEQTGTLTGIFGGMVWFLAIFGGAVADKLGFRRALSLAYAILAGAYFLLGSI
                     GAPWLAPVRSAVPLSFFVGVILILPALGISLVKPCVVGTTARASRENVRSLGYSIYYT
                     MVNIGGLLGPFAGSLARRYLGAGKIFLIAAAAVFAMFFFVLAFFREPKHLDEKPMPTI
                     AETLRNFGTVLSNPKFMLFLLIFTGYWIVFWQQWIVMPVYLHEYVNKDIDVERILMTD
                     SIVVITCTILVNYLTKKVPAFSAIIIGTFITGISWMILAFYPSMWGAIATIAVLAIGE
                     IVHQPRYYEYVSRLAPPGQQGTYMGFAFVPLGIGSLVGGWFGGKVLHHFGEVLNKPQW
                     TWFVISGVGVVTTLLLLVYDRTVRPKSAEVPAA"
BASE COUNT          773 a          905 c          921 g          694 t
ORIGIN      
        1 cacgcagcgc ctggaccctg gccgcgcgca gcagaaaagc cgctaaaaca gcggcgtttt
       61 ccacaccatc ctgaaaaaaa cagcgttttt acggcatttt tgcggcaatt gcccttgcgt
      121 tcccgtgccg tgttcgctat agtcccacac ggctcgagtt ttttcgcccg gattcagggt
      181 agacgaaaaa attcccgctg gtcccgagtt cctcgggacg gaaagaaatt tacaggagga
      241 taggcagact agatggcagc taagccactc agcaagacca agatcgttgc gcacatcgcc
      301 gagaagctcg gcacaccgaa gaagcaggta gctacctttt tcgaggagct gcacaagctg
      361 gcggtgaagg aagccaaggg cggcgcgggt aaatttgtca tcccgggaat cgggcgcgtg
      421 gtcaaggcgc accgcaaggc gcgcatgggc cgcaatccgc agaccggcga agcaatcaag
      481 atcaaggcca agaccgtggt gcggctgcgc ccggccaagg ccctgaaaga cgccatcctg
      541 aagtagggtt ccagggtttg ttttacaact tcttttcgca ggaccgattt tgtcctgaag
      601 ccctgagtaa ataaacgggc cctcctgcac agggaagcat agaaaaattt gcccccgtgg
      661 tcacgataac ccacgggggc tttttccttc tgcgactgcg gtttgtcgcg gatagcgctg
      721 gagtgagttc ttacagagtc ttgacgtagg cgatcacgtc tttgatctgg ctgtcgtcaa
      781 tcacgtcttt gaacggcggc atcaacgagt cgccgttttc gatccaagtc ttcagattgt
      841 catccgtgac tttgttgttg ttcacggtga aggtgccgcg tttgttgagt cccttaagcc
      901 cgggcccgat tttcttggcg tcgctggtgt cgtaatggca ggtcgagcac ttctgctgga
      961 acagctcttt gccacgcgct gcagcggccg cgtctgtttt tcccgcggga gatttctttg
     1021 tggtcttcgt ttgcgccaag cccacgccag cgacagcgag cgaggttcca agagcggcga
     1081 tccccagtac ggccagaatg cgattcacga ttctctcctg ttagtcgtgc gtagcacgct
     1141 tgttcacttc ttattctctc tgtttgatgc ctgaaattgc aattcttctg caaaacggga
     1201 cacttattct gcgaaaaaac tgcttgcacg cccgatagga ggtacaggtt caccttgcgc
     1261 gccattggga cttccgtacc gtaggcagca tacgtcccga gtggtaacaa tgacggcatg
     1321 aatccggaga cccttacgca ggcgtgggaa gttttgcagg acgcatatca ggcgcagatg
     1381 gaaggcgact acgaacgcgc cgtggagctg tatcaatctt cactggatct gcatccgaca
     1441 gctgaggcgc atacttttct cggttggacc tatcactttc agggcaggct ggaagacgcc
     1501 atcgcggaat gcaagcgagc gattgagatc gatcctgatt ttggcaatcc ctataacgac
     1561 attggcgcct acatgatcga gcagggcaac ctggatcagg cgattccctg gctgcagcag
     1621 gccattgact ccaaacgcta tgaaccccgg catttccctc actacaatct gggccgagcc
     1681 tatttgggca aggaaatgta ttcgcaagcc atgcgctgtt ttcaggaagc gttagaaatc
     1741 gagccgcgct acggcttggc gcgccaggcg ctggactcgc tgcgccgcat gttgaattaa
     1801 gcggctggta cctccgctga tttcggccgg accgtacggt cgtaaaccaa caacaaaagc
     1861 gtagtcacca cgcccacgcc gctgatgacg aaccacgtcc actgcggctt gttcaggact
     1921 tcgccgaaat gatgcaacac ttttccgcca aaccatccac cgactagcga accgattccc
     1981 agcggcacga atgcaaaccc catgtaggtt ccctgctgcc ccggcggcgc cagccgcgaa
     2041 acatattcgt aatagcgcgg ttgatgaacg atctctccga tggcgagcac cgcgatggtt
     2101 gcgatcgctc cccacatgga cgggtaaaat gcgaggatca tccaggagat tcctgtgata
     2161 aacgtaccaa taatgatggc gctgaaagcc gggacttttt tggtgagata gttcaccaga
     2221 atcgtgcagg tgatcacgac aatgctgtcg gtcatcagga ttcgctcgac gtcgatatcc
     2281 ttgttcacgt attcgtgtaa atagacgggc atcacgatcc actgctgcca gaagacgatc
     2341 caatagcccg tgaaaatcag taagaaaagc atgaactttg gattgcttag gactgttcca
     2401 aaatttctga gcgtctccgc aatcgttggc atgggttttt cgtccaggtg cttcggttcg
     2461 cgaaaaaagg ccaaaacaaa aaagaacatg gcaaacactg ccgctgccgc gatcagaaaa
     2521 atctttccgg cgcccaagta gcgcctggcc agcgatccgg cgaacgggcc gagcagcccg
     2581 ccgatattca ccatggtgta gtaaatggag tagccaagcg agcgcacatt ttcgcgcgaa
     2641 gctcgagccg tcgttccgac aacgcagggt ttgacgagcg aaatgcccaa cgccggcaag
     2701 atcagaatga ccccgacaaa aaaactgagc ggaaccgccg agcgcacggg cgctaaccag
     2761 ggggcgccga tcgatccgag caggaagtac gcgccagcca gaatcgcgta agccagcgaa
     2821 agggccctgc gaaaaccaag tttgtcggcc acggccccgc cgaaaatggc gaggaaccac
     2881 accataccgc cgaagattcc cgttagcgtg ccggtctgct cagttggaaa actcagcgtc
     2941 tcgcgcaggt aattcgctag tgatgcgaaa gcgccgtagt aggccagccg ctcgaagatt
     3001 tcgctgatgt tggcgaccca gaaggggcgc tcaaatccgg tgcggacgtc ctgcaggcgt
     3061 tgttgaaagg acatgtaccc tcatggcggc agccgctcgg gaaaggaacg cagcgggcgc
     3121 ggcgggatga taacagagga aaaggacaga ggcgcgtgtt cccatttggg aagtggtggc
     3181 gaggccaccc ctctcccttt tttttaagta ttgattctac tgagcttagc agccacgtat
     3241 tttgtatgca cttagcgagc aaccaaaagt ttagaaagag tggtgcacaa ttc
//
LOCUS       DASTPM010000286         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1336218, whole genome shotgun sequence.
ACCESSION   DASTPM010000286 DASTPM010000000
VERSION     DASTPM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336218"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..913)
                     /locus_tag="VFR42_05060"
     CDS             complement(101..913)
                     /locus_tag="VFR42_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0368553.1"
                     /translation="MLEIARVSKTFNAATPNELRALRGIDLKVQEGSFVAVIGTNGSG
                     KSTLLNAVAGSFFVDSGAISVGGEDITRWAERRRAKFIGRVFQNPFSGTAASMSIAEN
                     LALAAYRGKRRGMSWLLSKNLLSSMRERVRELNMGLEDRLKNAIGSLSGGQRQALTLL
                     MATWLRPQVLLLDEHTAALDPKSADQVIALTDAIVKREKLTALMVTHSMQQAASLGDR
                     LIMMHRGQLIQDFCGAEKKRLRPEELMARFEEVRRAELLDESAGVMLRRMYV"
     gene            complement(906..1850)
                     /locus_tag="VFR42_05065"
     CDS             complement(906..1850)
                     /locus_tag="VFR42_05065"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368554.1"
                     /translation="MSLLIGALSLGFIFSLIALGVLISFRVFSFPDITADGSSTLGAA
                     VAAVLLVNHLPAPIATVAGFLAGFLAGALTGTLATKCRINGLLSGILVMTGLYSVNLR
                     IMGRSNVSLSGASTLANFAERVGGALMGRTTFSVMGWEVASREASILLAAVAFIVLAG
                     CVMYVFLQTNLGTAMRATGDNPLMVRSLGVSDDNMIILGLAVSNGLVGLGGALLAQYQ
                     GFADVQMGIGMVVWGLASVIIGEALVGTRQLGLSITGAVMGSVLFRLLVAIALRVGLN
                     PNDLKLATAVFVFLALVAPSLLQRPRPFGQKLAGAPNA"
     gene            complement(1847..>2373)
                     /locus_tag="VFR42_05070"
     CDS             complement(1847..>2373)
                     /locus_tag="VFR42_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368555.1"
                     /translation="LSALLDSCLELAETQQAGVVMIAESAGLVGAALRRSPVRAHAVG
                     DVFVFPQVREWLSFTAEPAHTKSVVLAAGVALRGECEELKDWVRPLCTASAKKPPVLG
                     HLHMAAFSYRPVPRGKIELKESVRALFERQSLEGILHLLTDDRGGTRRESEFVRGACW
                     IAPIVRFAGEEARG"
BASE COUNT          492 a          825 c          608 g          448 t
ORIGIN      
        1 agatgttcat ggagatgtcg gtttgccgtc aatttcttgc cgcacccttt gttttgcgtc
       61 cgacatcggt cgcgccgttt tgcgcgttcc acgaaaccac ttagacatac attcgccgca
      121 gcattacgcc agcgctctcg tccagtagtt ccgcccgccg cacttcttca aagcgcgcca
      181 tcagctcttc cggccgcagc cgcttttttt ccgcgccgca gaagtcctga atcagctggc
      241 cgcgatgcat catgattaac cggtcgccca ggctggccgc ctgttgcatg gaatgcgtca
      301 ccatcagcgc cgtcagcttc tctcgtttca caatggcgtc ggtcagcgcg atcacctggt
      361 cagcactttt cggatccagc gcggcggtgt gctcatcgag tagcagcacc tgcgggcgca
      421 gccaagtcgc catcagcagc gtcagcgcct gccgctggcc tcccgaaagg gacccgatgg
      481 cattcttcag ccggtcttcc agtcccatgt tcagctcccg gacgcgctcc cgcatgctac
      541 tgagcaaatt tttgctcagt agccagctca ttccccgtcg tttcccgcgg tatgctgcca
      601 gcgccaggtt ttccgctatg gacatgctcg ccgccgtgcc gctgaaggga ttttggaaaa
      661 ccctgccgat aaacttcgcg cggcgccgct cagcccacct ggtaatgtcc tcgcctccca
      721 cgctgatggc tccggagtcc acgaaaaacg atccagccac cgcgttcaag agcgtggatt
      781 tcccggagcc gtttgttccg atcactgcaa cgaacgagcc ttcctggact ttcaagtcaa
      841 ttcctcgcag cgcccgcagt tcgttgggtg ttgcggcgtt aaacgtcttg ctgacgcgcg
      901 cgatctcaag cattgggagc cccggccaac ttctgcccga acggcctcgg tcgctgcagg
      961 agtgaaggag caaccagcgc aagaaacacg aacaccgccg tggccaattt caggtcgttc
     1021 gggttaaggc ccacccgcaa cgcaatcgct accagaagac ggaataacac cgagcccatc
     1081 accgccccgg taatgctcag tccgagttgc cgcgttccta ccagcgcctc gccgataatc
     1141 acgctggcca agccccacac caccatgccg atgcccatct gcacgtccgc gaatccctga
     1201 tattgcgcca acaacgctcc gcccaggcca accagtccgt tcgaaacggc caaaccaaga
     1261 atgatcatgt tgtcgtcgct tactccgagc gagcgcacca tcagcgggtt atcaccggtt
     1321 gcacgcatgg ccgtgcccag gttggtttgc aagaaaacgt acatcacgca acccgccagc
     1381 acgatgaacg ccaccgcggc caatagaatg gatgcttcgc gcgacgcgac ctcccaaccc
     1441 atcacgctaa acgtcgtccg gcccattagc gcgccgccaa ctcgctccgc gaagttggct
     1501 agcgtcgaag ctcccgacag cgacacattg cttcggccca tgattcgcaa gttgaccgag
     1561 tacagccccg tcatcaccag aatgcccgag agcagtccgt tgatccggca ctttgtcgcg
     1621 agagtgccgg tgagcgcgcc tgccagaaat cctgccagga aaccggcgac ggttgcgatg
     1681 ggggcaggca agtggttcac cagcagcacc gccgccaccg ccgctccgag cgtgcttgat
     1741 ccatccgcgg taatgtccgg aaacgaaaac acccgaaaac tgatcagcac gcccagcgcg
     1801 atcagcgaaa aaatgaatcc cagcgacagc gcgccgatca gcaggctcat ccgcgcgcct
     1861 cttcgcctgc aaacctcaca atcggcgcga tccagcacgc gccccgcaca aattcgcttt
     1921 cccgacgcgt tccgcctcgg tcatcagtca acagatgcag aatgccctcg agcgattgcc
     1981 tttcaaacag cgcgcgcacc gattccttca gttcgatttt tccccgtggc accggacggt
     2041 aagaaaatgc cgccatgtgc aaatgtccca aaaccggtgg cttcttagcc gatgcggtgc
     2101 acagcggccg cacccagtcc tttaactctt cgcactctcc ccgtagcgca actcctgcag
     2161 ccaacaccac actcttagtg tgcgcaggtt ccgctgtgaa actgagccat tcgcgaacct
     2221 gaggaaatac aaaaacatcc cccacggcat gtgccctgac cggcgaccgg cgcaacgcgg
     2281 ctccaacgag cccggccgac tcggcgatca ttaccacacc ggcttgctgg gtctccgcca
     2341 gttccaggca agaatcaagt agcgcggaaa gcg
//
LOCUS       DASTPM010000287         6589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_644309, whole genome shotgun sequence.
ACCESSION   DASTPM010000287 DASTPM010000000
VERSION     DASTPM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644309"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFR42_05075"
     CDS             <1..573
                     /locus_tag="VFR42_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368556.1"
                     /translation="LLACGLLAGASGTKIAFAQEKKDAGTGAAALSGKSAADMPAAKP
                     ADVKTLDAIVAAVYDVISGPPGERDWNRFNSLFTKDARLIAVRTPKDGGKPSLAVMTP
                     ADYAAHAGGYFKEHGFFEHELSRKTDSFGAMTHVYTTYESRETKDGKPIDRGINSMEF
                     FFDGERWWCVQIYWDAERPGNPIPEKYLGK"
     gene            complement(657..2180)
                     /locus_tag="VFR42_05080"
     CDS             complement(657..2180)
                     /locus_tag="VFR42_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368557.1"
                     /translation="MKRFGAACALALAVICWTAGCNDYGNTFQSPTGASLVSLSPSNA
                     SAGGDTFTLNLFGGGFVPKTVVQWNGHTIPTTVTTDVNNNVLTLTATVDKSLIATTGS
                     ARVNTLNPHSGSTDNGLSNTIVFIINPQPNPLPVLSSISPTSAAPGSASLALTLTGSS
                     FIPSSDPTGGSQVRWNANGTQTTLATTSVSASQITATVPADLLTAQTCAIVSVNNPPA
                     QGTAPPSGVSNPFNGGGGTSASTPGFAVSTDSTFVSNCNALGANAAKTSALAVAEETP
                     ALGSDGRFVSYTAAQNGHSQIFLRDTCTGAATDCQPRTTLLSASADGSAGNADSNTPS
                     MSVDGRFVAFSSAATNLVANTPAGRQVFVHDTCFGAATSCTPQTTLASTDEGGALSGN
                     DNLLPSLSSSGRFVAFLSVTPSKYPSKSETANSGYRQIFVRDTCFGATSSCTPKTTRL
                     SVMPGDTNSLQSKPAGPAISGSAQAVGVSSATAPTVLTRTVPVDDRVFLALTNSHQQ"
     gene            2552..3976
                     /locus_tag="VFR42_05085"
     CDS             2552..3976
                     /locus_tag="VFR42_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0368558.1"
                     /translation="MAETKKELTKAHAPQHASAHPTGGLERGLGLKEAVALNMIEIVG
                     IGPFVVSGVVIKAMGGPQALLAWLGGAVLATLDAFVWSELGAAMPKAGGTYVFLREAY
                     GPQRWGRLMSFLFVWQTFVQAPLSVASASIGFTRYAAYLHPLSGWQAKAVSGGLVILL
                     VILLYRRITTIGKISLLLWVGVVGTLLWLIWGGARHFDAKVAFDFPPGAFSFSWVWFA
                     GLGSAMVSTIYSYWGYYNICHLGGEIREPEKNIPRGIFLSVLGITILYLAMQTSILGV
                     VPWREAENSKFIVSTFVERLYGHTAAMVATGMVLWIALASVFSVLLGYSRVPYSAALD
                     GNFFPIFGRLHPTKHFPHISLLALGALAFFFSVKLDLETSIAGILAVRLIVQFIGQAV
                     GVMLLRRRFGTERLPFKMWLYPVPAILTMVGWAWLFWHTGKARKWGLLEIVMGVLAFL
                     VWTAAMKQWPFAKNANPPDQVEAG"
     gene            4079..>6589
                     /locus_tag="VFR42_05090"
     CDS             4079..>6589
                     /locus_tag="VFR42_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU0368559.1"
                     /translation="MKTCRAGVVFAVLMLLAGVPGRAQFPPAPGTGPGLAETIEAIDS
                     APVTTRILYVTAHPDDESAAVLTYLARGLHADVALLSLTRGEGGQNALGPEQAPQLGL
                     IRTQELLAATRGYGVKLYFTRAKDFGFSKTPEETEKVWGDQVMEDMVRVIRTFRPNIV
                     VNNWGDVHGGHGHHQAAGLLTPKAVTLAADEKAFPAQLQNEGLRAWGSKQNPGTILDL
                     ERGVEKPRGYVLPLDEVSPLWGKTWREIGMDAFANHRTQGVAVFFGNPFFRRPISLKK
                     EDGTAPDPGSFAASLHAVAGDGEGANCKAAAFFCPVLKSAEQNIAAAGTSALSLQWKE
                     TTALLARAAQQIASLYPGGSHPAHWVANDPLLDELKLAEKRVQRALNLSAGLTISATA
                     DKSALIAGEPCVVTTQKKCRPGIDCEFLAARIDAPSGWTETKAGNGSEAEKIQVTPGK
                     DGRENSAWSEQIPEPPPFLSVVQDVRVEGYTYKTEVPVTHIESSSTRVDRVPLRLVPA
                     YTLSVEPKQLARLIENNGKPFEMFIRVHSYSARKEDVSVGLDLPSGWTSGGDSKLTFA
                     GAGDAYTKIVVTPPAALTAGNYRLAVHIKRGAEIFATSLEPLPSVPTLLWQESAQCVV
                     HAFDINVPKNLRVGYITAESEPVPDSLRMLGIKVDLLDAAALNFGDLSRYDAIVVGVR
                     AYELRSELPGANQRLLDYVSNGGTLVVQYERDFAWDGKNYAPYPAKIDGGQAGQPLPR
                     ITDETAAVNFLKPDDPLLNKPNKITAKDFDGWVQERGLYYWTDFDTSKYTPLLSMHDP
                     GEKDLNGGLVYTKLGKGTYIYTGLAFFRQLPEGVPGAYR"
BASE COUNT         1384 a         1877 c         2029 g         1299 t
ORIGIN      
        1 ttgctggctt gtgggttgct ggctggtgcg agtgggacca agatcgcgtt cgcgcaggaa
       61 aagaaagatg ctgggactgg cgcggctgcg ctgagcggaa agagtgcagc ggatatgccg
      121 gccgcgaaac ctgcggatgt aaaaaccctg gatgcgattg tggcggccgt ttacgacgtg
      181 atttcagggc cgccgggcga gcgcgactgg aaccggttca actccctgtt cacaaaagac
      241 gcgcggctga tcgcggtgcg cacgccgaaa gatggcggga agccaagcct agcggttatg
      301 acaccggcgg attacgcggc gcatgcgggc ggatatttca aggagcacgg attttttgag
      361 cacgaactga gccgcaagac cgattcattc ggcgcgatga cgcacgtcta cacgacatac
      421 gaatcgcgcg aaacgaagga cggaaaaccg atcgaccggg gcatcaacag catggaattt
      481 ttctttgacg gcgagcgctg gtggtgcgtg cagatttatt gggacgccga gcgcccggga
      541 aatccgattc cagaaaaata tttgggcaag taaagagcaa cagcgcagca aagaaaaaac
      601 cggcccggcg ctcgggagga gtgcccgatc gctgagcaat cccgctactt caacacctat
      661 tgctgatggg agttggtaag ggcgaggaaa acgcgatcgt ccaccggcac cgtgcgtgtc
      721 aaaacggttg gtgcggtggc actggaaacg cccacggctt gcgcggaacc tgagatggca
      781 ggaccggcgg gcttgctctg cagcgaattg gtgtcgccgg gcatgaccga aagacgagtc
      841 gtcttgggtg tacaggaact ggtggcgccg aaacaagtgt cgcggacaaa aatctgacgg
      901 tagccgctgt tggctgtttc ggatttcgaa ggatatttcg acggcgtaac cgacagaaac
      961 gcgacgaagc gcccggaaga gcttagcgac ggcaaaagat tgtcgttgcc ggagagcgcg
     1021 ccgccttcgt ccgtggaggc gagcgtcgtc tgtggcgtgc aagatgtggc cgcgccgaaa
     1081 cacgtgtcgt gcacaaagac ttgccgtccc gcgggcgtat tggcgacgag attggttgcc
     1141 gcggaactga aagctacaaa gcggccgtcg acgctcatgg acggagtgtt gctgtcggca
     1201 ttcccggcac tgccatcggc gctggcggaa agcagggtgg tgcgcggctg gcaatccgtc
     1261 gccgcgccgg tgcaggtgtc gcggagaaaa atctgcgagt ggccattttg cgcagcggta
     1321 taggaaacaa aacggccgtc agacccaaga gccggcgttt cttccgcaac ggcaagcgct
     1381 gaagttttgg cagcattcgc gcctagcgcg ttgcagttgg aaacaaaggt cgagtccgtg
     1441 gacacggcga atcctggagt gctcgcactg gtgccgccac cgccgttgaa gggattgctg
     1501 acgccgctgg gcggagcggt tccctgcgcc ggtggattat ttacggaaac aatcgcacac
     1561 gtttgcgcgg tgagcagatc ggccggtacg gtcgctgtaa tttgcgaggc gctgacgctg
     1621 gtagtcgcca gcgtagtctg cgtgccgttg gcgttccatc gaacctgcga gccaccggtg
     1681 ggatcggaag agggaatgaa actcgagcca gtaagggtca aagccaatga agcgctgcca
     1741 ggagccgcgg aggtgggcga aatggaagac aacactggca acggattcgg ctgaggattg
     1801 atgatgaaaa cgatggtgtt cgacagcccg ttgtccgtgg atccggaatg tggattcaag
     1861 gtgttgactc gggccgaacc ggtcgtagcg atcagcgact tatccacggt ggcggtaagc
     1921 gtgaggacgt tattgttaac atctgtagtg acggtggtgg ggatcgtatg gccgttccat
     1981 tgaaccaccg tcttcggcac aaagcccccg ccaaaaaggt tcagcgtgaa ggtgtcgccg
     2041 cccgcgctgg cattcgaggg agacaaagag acgaggctgg ccccggtagg cgactgaaac
     2101 gtattgccgt agtcgttgca acccgcagtc caacagatca cggcgagcgc taatgcgcac
     2161 gctgccccaa accgcttcat gagaactccc tttatgtcta caggctttca gcggggaacc
     2221 cgccgagcca gccagctgac tgctgaggtg agatgcgaaa agcaacgggc gcgttgactg
     2281 gacgcagata cgcaaaaacc tgctctgcgg tcactcgaaa ttgctaggca atcacgcgaa
     2341 aaactcgacc ggccggctgg cctgtaactg gcactgcagc cggggcttcc ccgacgcatc
     2401 ttcccgaacc ctgcctcctt atcatccatt gcaggcgggg aaaggtcaat gcagagcaca
     2461 tcttcattcg ttcgccccat aagttgaccg aatcggcact ggcccgcgcg ccacgaaaag
     2521 caagcgagaa gctgctatcc tcagcagcgc gatggcggag acaaaaaaag aattaaccaa
     2581 ggcacatgct cctcagcacg cgagcgcgca cccaaccggc ggactggagc gcgggctcgg
     2641 cctgaaagag gccgtcgcgc tcaacatgat tgagattgtc ggcatcggcc cattcgtcgt
     2701 gagcggcgta gtgatcaagg ccatgggcgg gccgcaagcg ttgctggcat ggctgggcgg
     2761 tgcggtgctg gcgacgctcg acgcattcgt ctggtcggag ctgggcgcag ccatgcccaa
     2821 agccggcggc acctacgttt ttttgcgtga agcttatggg ccgcagcgct ggggtcgcct
     2881 gatgtctttc ctcttcgtct ggcaaacgtt tgtgcaagcg ccgctgtcgg tcgcgtcggc
     2941 gtccatcggg ttcacgcgct atgcggcgta cttgcatccg ctttccgggt ggcaagcaaa
     3001 agcagtctcg ggcggtctgg taatcctcct ggtgattctg ctttaccggc gcatcaccac
     3061 catcggaaaa atttctctgt tgctgtgggt cggcgtggtc ggcactttgt tgtggctcat
     3121 ttggggtggc gcgcggcatt tcgacgccaa ggtggcgttc gattttccgc ccggcgcgtt
     3181 ttcgttctcg tgggtatggt tcgccgggct gggctcggcc atggtcagca cgatttacag
     3241 ctactggggc tactacaaca tctgccatct gggcggcgag attcgcgaac cggagaaaaa
     3301 tattccacgc ggcattttcc tctcggtgct gggcatcacc atcctgtacc tggcgatgca
     3361 aaccagcatt ttgggcgtcg tgccatggcg cgaagcggaa aattccaagt ttatcgtcag
     3421 cacattcgtg gagcggcttt acggccacac agccgcgatg gtcgccaccg gcatggttct
     3481 ttggatcgcc ctggcctctg tgttttcggt gttgctggga tattcgcgcg tgccctattc
     3541 cgcggctctc gacggaaact tcttcccgat ctttggccgc ttgcatccga cgaaacactt
     3601 tccgcatatt tcgctgctgg cgctgggtgc gctggcattt ttcttcagcg tgaagctcga
     3661 tctggaaaca tcgatcgcag gcatcctcgc cgtccgcctg attgtccagt tcatcgggca
     3721 ggcggtcggc gtaatgttgt tgcgcagaag gttcggcacc gaacggctgc ctttcaagat
     3781 gtggctgtat cctgttcccg ccattttgac catggttggt tgggcctggc ttttctggca
     3841 taccggcaag gcccgcaagt ggggcttgct ggaaattgtg atgggcgtgc tcgcctttct
     3901 ggtctggacg gccgccatga aacagtggcc gtttgccaag aacgccaacc cgccggacca
     3961 ggtagaggca ggctaaaccg aaaatgcgcg gcatacatct catctttgtt atcaaacgcg
     4021 cttatgagca gtcgccggag tggatgaaat tttcgcaact gagttggaag ggggacgagt
     4081 gaagacttgc cgcgcgggag tcgtctttgc agtgttgatg ttgttggccg gggtacccgg
     4141 gcgcgcgcaa tttccgccgg ccccgggcac cggtcccggc ctcgccgaaa ccattgaagc
     4201 gattgacagc gcgcccgtca ccacgcgcat cttgtatgtc accgcgcatc ccgacgacga
     4261 aagcgccgca gtactcactt atctcgcgcg tgggttgcat gcggacgtag cactgctctc
     4321 gctgacccgc ggcgaaggcg gccagaacgc gctcgggccg gaacaagctc cgcaactcgg
     4381 actgattcgt acgcaggaat tattggcagc aacacgcggc tacggcgtga agctctactt
     4441 tacccgagcc aaggactttg gcttttccaa aacccccgaa gagaccgaaa aagtctgggg
     4501 cgaccaggtg atggaagata tggtgcgagt catccgcacc ttccgcccca acattgtcgt
     4561 caacaactgg ggcgacgtac acggcgggca cggccatcac caggcagcgg gcctgttgac
     4621 gcccaaagcc gtcaccttgg ctgccgatga aaaagccttc cccgcgcagc ttcaaaatga
     4681 agggctgcgc gcgtggggaa gcaaacaaaa tccgggtacc atcctcgatc tcgaacgcgg
     4741 agtcgaaaag ccgcgtggat acgtcttgcc cttggacgaa gtttctccgt tgtggggcaa
     4801 gacctggcgc gaaattggaa tggacgcctt cgcgaaccat aggacgcaag gcgtggcggt
     4861 tttttttggc aaccctttct tccgccggcc gatctcgcta aaaaaggaag atggcacggc
     4921 gcccgatccg ggatcgttcg ctgcttcttt gcacgccgtc gctggcgacg gggaaggagc
     4981 caattgcaag gcggcggcgt tcttctgccc ggttttgaaa tccgcggaac aaaatattgc
     5041 tgccgccggc acctcggcgc tttctctgca atggaaggag acaaccgcgt tactggcgcg
     5101 cgctgcgcaa caaattgcgt ccctctatcc cgggggatcc caccccgccc actgggtagc
     5161 gaacgatccg ctgctcgacg aactgaaact cgccgagaag agggttcaac gtgcgcttaa
     5221 cctctcggcg ggactaacga tttccgccac ggccgacaaa agcgcgctga tcgcgggaga
     5281 gccctgtgtt gttaccacgc agaaaaagtg tcgcccagga attgactgcg aatttctggc
     5341 cgcgcgcatc gatgcgccat cgggatggac agaaacaaaa gctggaaacg ggtctgaggc
     5401 agaaaagatc caagtcacac ctggaaaaga tggccgagaa aattccgcgt ggagcgagca
     5461 gattcccgag ccccctccat tcctttccgt cgtgcaggat gtaagagtgg aaggctatac
     5521 ctataaaacc gaggtcccag ttactcacat agaatcctcc tcaacaagag ttgaccgcgt
     5581 gccgctgcgc cttgtcccgg cttacacgct tagtgtagag cccaagcagc tcgcccgcct
     5641 cattgagaac aatggcaaac cgttcgaaat gtttattcgt gtccactctt attccgccag
     5701 aaaggaagac gtgagcgtcg gattggatct tccttccggc tggaccagcg gtggcgactc
     5761 gaaactgacc tttgcgggcg cgggggatgc gtacacaaag attgtcgtga cgccacccgc
     5821 tgcgttgact gccggcaatt accggctcgc agtacacatc aaacgaggcg ctgaaatttt
     5881 cgccacttcg ctggagccgc tgccctccgt gcccactttg ctctggcaag agtcggcgca
     5941 atgcgtcgtg cacgccttcg acatcaacgt tcccaagaat ctgcgcgtcg gctatatcac
     6001 cgcggaaagc gagcctgtcc ccgattcgct ccgcatgctc ggcattaaag tggatttgct
     6061 ggacgcggcc gcgctgaatt ttggcgacct ctctcgctac gacgccatag tggttggcgt
     6121 gcgcgcttac gaactccgca gcgaactccc cggcgcgaac cagcgcctgc tcgactacgt
     6181 ttcgaacggg ggaaccctgg tggtgcaata cgaacgcgac tttgcctggg acggcaaaaa
     6241 ctacgcgccg taccccgcaa aaattgacgg cggccaggct ggccagcctc tgccacgcat
     6301 caccgacgaa accgcggcgg tgaacttcct caagcccgac gatccgcttc tcaacaagcc
     6361 caacaaaatc accgcaaaag acttcgacgg ctgggtgcaa gaacgcgggc tctactattg
     6421 gactgacttc gacacttcaa aatacacgcc gctgctttcc atgcacgatc ccggtgaaaa
     6481 ggatctgaac ggcggactgg tttacacgaa gctcggcaag ggaacctata tctacaccgg
     6541 cctcgcgttt ttcaggcagc tgccggaagg cgtccccggc gcctatcgc
//
LOCUS       DASTPM010000288         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1307629, whole genome shotgun sequence.
ACCESSION   DASTPM010000288 DASTPM010000000
VERSION     DASTPM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307629"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VFR42_05095"
     CDS             complement(<1..1050)
                     /locus_tag="VFR42_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0368560.1"
                     /translation="MPERTRAQIPGHWAGPHRSGHVVPGWILSFHDPELTALVADAVE
                     RNPDLKAAAARVEASRAAVRIAASSLYPRIAMKGLGERQGQELRGDLGRGINPPDFGS
                     SGTENAGGAGLDTSVDESSRRWVYGIAAGAAWEADVWGRIRSKKAAAKAESLALEADY
                     EFARESLAAAVARAYFTTIEAAQQEANAQETLDIYQEYSKLTDIQKQHGFASDFDVSQ
                     IKSRTASAEDALYTAQQVRAQTIRAIEVVTSHYPAGRFDVRRSFPGQPRAVPAGLPAQ
                     ILERRPDLIASERRFAAAFHRVNEARTARLPRFVLSAGSGLGTAQLDGVGTLDAVLWT
                     FAAGVTQPIFFGGELQ"
     gene            complement(1156..>2027)
                     /locus_tag="VFR42_05100"
     CDS             complement(1156..>2027)
                     /locus_tag="VFR42_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU0368561.1"
                     /translation="VEPNRPIKKGDVLFKIDPVPFEAAEKAAEATLQGAKDQLNNAAN
                     KKASLTPRIELAKKRVEQFTALATTGAGKRADLEQAQSDLGNLQSEFLAADATESQAR
                     AQISKSEADLINAKFDLEGTTHLAPSNGRVANLAVQPGYRAVTLPAFPVMSFIQEDDP
                     WILAFFRQNELRYVEPGNEAEIFMKMYPGRIIKCKVDSILWATAQGQMPISGNLPNVA
                     PTGAPEQRIAVRLLLDGRDSNVFLAAGARGQGTIYTNDITPIQILRRIIVRIAAKFDW
                     FVIKHLSALFGAH"
BASE COUNT          355 a          611 c          637 g          424 t
ORIGIN      
        1 ttgaagttcg ccgccgaaga aaatcggttg agtgactcca gcggcgaaag tccaaaggac
       61 ggcatcgagg gtcccaaccc catcgagttg agcagttccg agtccgctcc cggcactgag
      121 cacaaaccgc ggcaggcgag cagtgcgagc ctcgttcacc cgatgaaacg cagcggcgaa
      181 gcggcgctcg ctggcgatga ggtccggccg gcgttcgaga atttgcgcag gcagacctgc
      241 gggcaccgct ctcggttggc cggggaacga acgtcgtacg tcaaaacggc cggccgggta
      301 atggctggtg acaacttcga tcgcgcggat tgtttgcgcc cgcacctgct gtgcggtgta
      361 gagcgcgtcc tctgcgctgg cggtgcgcga cttgatctgc gacacgtcaa agtcgctcgc
      421 gaagccgtgc tgcttctgga tatccgtgag ctttgaatac tcctggtaga tgtcgagcgt
      481 ctcctgcgcg ttggcttcct gttgcgcggc ctcgatggtg gtgaagtacg cgcgtgccac
      541 cgccgcggcg agcgattccc gggcaaattc gtagtcggct tccaacgcaa ggctttccgc
      601 ttttgcggca gccttttttg agcgaatgcg tccccaaaca tcggcctccc atgcagcgcc
      661 ggcggcgatg ccatacaccc agcgccgtga tgattcatct acgctcgtgt cgaggccggc
      721 gccgccagcg ttctccgttc ccgagctccc gaagtccggt gggttgatgc cccggccgag
      781 atcgccgcgg agttcctgtc cctgccgttc acccagtccc ttcatcgcaa tgcgcggata
      841 gagcgatgat gcagcaattc gcaccgcagc gcgcgatgct tcgactcgcg cagcggcagc
      901 tttaagatcg ggatttcgtt cgacggcgtc ggcaaccagc gcggtcagct ccggatcgtg
      961 aaagctgaga atccagcccg gcacgacgtg gccgctgcga tgcgggccgg cccagtgccc
     1021 cggaatctgc gcgcgagtcc tttcaggcat gatctcctcg ccaacgggcg cctgcctaat
     1081 cgcgcagccg gcaagtgcga ggcacgcggc ggcagcgaga ggcagggtgc agcgccgtcc
     1141 gttcatcgtt tgatcctagt gagcgccgaa gagagcggag agatgtttga tgacgaacca
     1201 gtcgaacttc gcggcgatgc ggacgatgat gcgacggagg atctggatcg gcgtaatatc
     1261 gtttgtgtag atggttccct ggccgcgggc cccagcggcc agaaaaacat tgctgtcccg
     1321 cccgtcaagg agaaggcgca cagcgatacg ctgttcgggc gcgccggttg gcgcgacatt
     1381 gggcaggttt ccgctaatcg gcatttgccc ctgtgctgtg gcccaaagaa tcgagtccac
     1441 cttgcatttg atgatgcggc ctggatacat cttcatgaag atttccgcct cgttccctgg
     1501 ctctacatag cgcaattcat tctggcggaa aaaggcaagt atccacggat cgtcctcctg
     1561 gataaagctc atcaccggga acgccggcag ggtcaccgcc cggtagccgg gctgcacagc
     1621 gagattcgct acgcggccat tggacggcgc gagatgcgta gtgccttcga ggtcgaattt
     1681 cgcattgatc aggtctgctt ccgacttgct gatctgcgct ctggcctgcg attccgtagc
     1741 atcggcggcg aggaattcgc tctggagatt gccgaggtcg ctttgcgcct gctcgaggtc
     1801 agcccgcttc ccggccccgg ttgtggcgag tgccgtgaat tgttcgacgc gttttttcgc
     1861 caactcaatc cgcggagtca aggaggcttt tttgtttgcg gcattgttga gttgatcctt
     1921 cgctccctgc agcgtggcct cggccgcttt ctcagccgct tcgaaaggca ccgggtctat
     1981 cttaaaaaga acatcgcctt ttttgatcgg acggtttggt tcaaccg
//
LOCUS       DASTPM010000289         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_932725, whole genome shotgun sequence.
ACCESSION   DASTPM010000289 DASTPM010000000
VERSION     DASTPM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932725"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFR42_05105"
     CDS             <1..334
                     /locus_tag="VFR42_05105"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU0368562.1"
                     /translation="VNSATHMWGSQRFETGDTSTNSFWVAMLTFGEGWHNNHHAVPQA
                     ARHGLAWYEFDINWYGIAALRFLGLAWDVKLQKPLSELEASPESQPRLLVARPVPVVE
                     PEAVNSGD"
     gene            435..1334
                     /locus_tag="VFR42_05110"
     CDS             435..1334
                     /locus_tag="VFR42_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0368563.1"
                     /translation="MRITSRTRAIVFFIALGVCLVGLALFLNVSWIVVNWRRDVPLVL
                     GIIFFGVIIAGVVVNTIFLVREIRRNEQQDSFLNAVTHELKTPIASIRLYLETLQSRG
                     IDDAQRREFYRIMLEDADRLLATVEQVLKAGEARQGSRPEHWQEVDFAKIVEETAELA
                     RLRHNLPPQTMRFGAEARGELLVMGKPEELRTAVFNLFDNAIKYSGEQKDIVVDLASA
                     ERDTIVLSVRDRGIGIPRPELKRIFSRFYRVPNSGQVKGTGLGLFIVRAVARRYGGDA
                     YAESEGEGRGSTFTISLPRAHRA"
     gene            1331..2074
                     /locus_tag="VFR42_05115"
     CDS             1331..2074
                     /locus_tag="VFR42_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368564.1"
                     /translation="MSRILVVEDEAHIAEGVRFNLEAEGHSVRVAGRGEEALHLLLEE
                     KEGFEALVLDVMLPGKDGFTVARELREAQNYIPLLMLTARSRPEDVLRGFESGADDYL
                     PKPFNLAILLARVQSLLRRKNWQNSAAPALEPVASESEAPEVFQFDDKTVDFQRLQLR
                     SGEQVFQMTLMESELLRYLIRNRGKAVSRKAILQDVWNLHEDTDTRAIDNFVVRLRRY
                     IEREPAKPRYLLTVRGVGYQFYPDPSKKV"
     gene            complement(2116..2958)
                     /locus_tag="VFR42_05120"
     CDS             complement(2116..2958)
                     /locus_tag="VFR42_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368565.1"
                     /translation="MSARKSVVRFLAHTLMVVGAIFVVTVFLAAMPGHNAPSIQAIGP
                     QQQPGGSQGASSSASQEMPGMDHSQMQIDAHEKANEKAAVTEMSHMHGGGPHMYMTEM
                     RAGNSADLERASEIVTQLRAGIEKYKDYQVALNDGYRIFLPNLPQPEYHFTSYKNGFM
                     AAMRFDPSQPTSLLYRKTSDGYQLVGAMYTMPERASEDQLNEHLPLSVARWHLHTNLC
                     MPPKGQRQTADWTKFGLQGSISTQAACEAAGGNFHPSVFGWMVHVYPYEDSLDKIFAM
                     HRRD"
     gene            3198..>3591
                     /locus_tag="VFR42_05125"
     CDS             3198..>3591
                     /locus_tag="VFR42_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368566.1"
                     /translation="MSKKTIHPAKTVAGGVAPPGDKSISHRYAMLAGIAEGTSELSNF
                     ALAADCHSTLGCMKALGAEVKVEKTLVRVTGRGLRGLQSSWRALDAGNSGTTIRLLSG
                     ILAGQSFTTKIAGDHSLNKRPMQRILTPL"
BASE COUNT          725 a          965 c         1127 g          774 t
ORIGIN      
        1 ggtgaattca gccacgcaca tgtggggctc gcagcgcttt gagacaggag atacctcgac
       61 caacagtttt tgggtggcga tgctcacgtt cggcgaaggc tggcacaaca atcatcacgc
      121 cgtgccgcag gctgctcggc atggtttggc gtggtacgaa ttcgacataa attggtatgg
      181 gattgccgca ctgcgctttc tcggtttggc atgggacgtc aagctgcaaa agccgctgag
      241 cgagttagag gcatctccgg aatcgcagcc gcgcctcctg gtagctcggc cggtgccggt
      301 agtcgaaccg gaagccgtca actccggcga ctaggacttc gcaaccgtca tggctatctc
      361 ccgaccatgc taactcttca tccctagttt catttcgtgc agcgatcccg ctggatggcc
      421 taaactagct gtccatgcgc atcaccagtc gaaccagggc gatcgtgttt tttatcgcgc
      481 tgggcgtttg cctggtggga ctcgcgctct tcctaaacgt cagttggatt gtggtgaact
      541 ggcggcgcga tgtgccgctg gtgttgggga ttattttttt tggagtgatc atcgcaggcg
      601 tggtggtcaa caccattttt cttgtgcgag aaatccgccg caacgaacaa caggacagct
      661 tcctcaatgc ggtcacacac gagctgaaaa cgccgatcgc ttccattcgt ctgtatctgg
      721 agacgctgca aagccgcggc attgatgacg cgcagcgccg cgagttttat cgcatcatgc
      781 tggaagacgc cgaccggctg ctggccaccg tcgaacaagt gctgaaagcc ggagaggcgc
      841 ggcagggcag ccgcccagag cattggcagg aagtggactt cgcaaagatt gtggaggaaa
      901 ccgcggagct ggctcgcttg cgccacaatt tgccgccgca gaccatgcgg tttggcgccg
      961 aagcgcgcgg cgaattactg gtaatgggaa aacccgaaga gctgcgcacg gctgttttca
     1021 atttgttcga caatgccatc aagtactccg gcgaacagaa ggacatcgtg gtggacctgg
     1081 cgagcgcgga gcgcgacacc atcgttttga gcgtccgcga tcgcggcatc ggcataccgc
     1141 gcccggaact caagcgtatc ttcagccgat tttaccgtgt acccaactcc ggccaggtaa
     1201 agggaaccgg cctggggctg tttatcgttc gcgctgtggc gcgccgatac ggcggcgacg
     1261 cttacgcgga aagcgaagga gagggccgcg gcagcacctt tacgatttca ttgccgaggg
     1321 cgcatcgggc atgagccgaa ttctggtggt tgaagacgaa gcacatattg cggagggcgt
     1381 gcgctttaac ctggaggccg aggggcattc ggtacgcgtc gccggacgtg gcgaagaagc
     1441 gctgcacctt ctgttggaag aaaaagaagg cttcgaggcg ctggtgctcg acgtgatgtt
     1501 gccgggcaag gatggcttta ccgtcgcccg cgagctgcgc gaggcgcaga actatattcc
     1561 gctactgatg ttgacggcgc gcagccggcc ggaggatgtg ctgcgcggat tcgaatcggg
     1621 cgcggacgac tatctgccca agccgtttaa cctggctatt ctgctggcgc gcgtgcaaag
     1681 cttgctgcgc cggaagaatt ggcagaactc cgcggctccc gctctggaac cggttgcatc
     1741 ggagtcagaa gcgcctgaag tctttcagtt cgacgacaag acggtggatt ttcagcggtt
     1801 gcaattacgc agtggcgagc aagtctttca aatgacgctg atggaaagcg aattgcttcg
     1861 ttacctgatt cgcaatcgcg gaaaagcggt ttcgcgcaag gccatactgc aggacgtgtg
     1921 gaatctgcac gaagacacgg atactcgcgc cattgacaat ttcgtcgtcc gcttgcggcg
     1981 ctacattgag cgggaacccg ccaagccgcg ctatctcttg acggttcgcg gcgtcggata
     2041 tcaattctat ccagatccca gcaagaaagt ctgagcctgc gatcgctcca tgttgtgccg
     2101 cagggcagct agtgtctaat cgcggcggtg catggcgaag attttgtcga gcgaatcttc
     2161 gtagggatag acgtggacca tccagccgaa gaccgaggga tggaaattgc cgcctgctgc
     2221 ctcgcaggct gcttgcgttg agatcgaacc ttgcaaacca aacttggtcc aatcggcggt
     2281 ttgccgctgg cctttggggg gcatgcagag attggtgtgc aggtgccacc tggccacgct
     2341 gagtggcagg tgttcgttca gttggtcttc gctggcgcgt tctggcatgg tgtacatggc
     2401 gccgacaagt tggtagccgt ccgacgtttt gcggtagagc agggaagtcg gctgcgacgg
     2461 atcgaaccgc atcgccgcca tgaatccgtt tttgtagctg gtgaagtgat attccggttg
     2521 cggaaggttg ggcaagaaga tgcgataacc gtcgttcagc gcaacctggt aatccttgta
     2581 cttctcgatg ccggcacgga gttgggtgac gatttcgctg gcgcgctcga ggtcagccga
     2641 attgcccgca cgcatttcgg tcatgtacat gtgcggaccg ccgccatgca tgtggctcat
     2701 ctccgtgacg gctgcctttt cattggcctt ctcgtgggca tctatctgca tctggctgtg
     2761 atccatgccg ggcatttctt gggaagcgga cgaggaggcg ccctgcgatc cgcctggttg
     2821 ttgttgcggg ccgatggctt gtatcgacgg agcgttgtgg ccgggcatcg cggccaggaa
     2881 aactgtgact acgaaaatgg cgcctaccac catcaaggtg tgggcaagaa atcgaactac
     2941 ggacttgcgg gccgacatga cttcgtttct ccagtcttcc tgtggcgctg ctgagcagcg
     3001 catcgggatg ctcttgccga gcatcctgct acaactatga tacggaagcg cgggtgccgg
     3061 tatttcagcg cgtttgtaag gaagtgcatg cggcaaaact gtccgccggg acagttgcaa
     3121 ccaggaaacc gcaagccgtg cgtgttaaaa tttcctcctg gtgaaatctg cggttaactc
     3181 ctcgtgagcg gactatcttg agcaaaaaga cgattcatcc ggcaaaaaca gtggcgggag
     3241 gtgtcgcgcc acctggcgac aagtccattt cgcaccgtta cgccatgctg gcgggcattg
     3301 ccgaaggcac cagcgaactg agcaactttg cactggctgc ggactgtcac agcacgctcg
     3361 gatgtatgaa ggccctaggc gccgaagtga aagtcgaaaa aacgctggtg cgcgtgaccg
     3421 gtcgcggctt acgtggcttg cagagtagct ggcgggcgct cgatgcaggg aactccggca
     3481 cgaccattcg gttgctgtcc gggattctgg ctggtcagag ttttacgacg aagattgcgg
     3541 gcgaccattc actaaataaa cggccgatgc aacgcatcct gacaccgctt c
//
LOCUS       DASTPM010000290         6417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1192563, whole genome shotgun sequence.
ACCESSION   DASTPM010000290 DASTPM010000000
VERSION     DASTPM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192563"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VFR42_05130"
     CDS             <1..791
                     /locus_tag="VFR42_05130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU0368567.1"
                     /translation="FQIKIGYPDKWRDYSALTVDKGSYLANERRATAFENARDLNKIG
                     KPVDRSEWQMTPPTVNAYYDAAMNEIVFPAGILQPPFYDPKADDAVNYGGMGAAIGHE
                     ITHGFDDEGSQFDAKGTLRDWWTKDDRKNFDERASCVQKQFDGYEVEPGLHENGKLVL
                     GESIADLGGLAISYAAYEKSIEGKRPPTLGGFTPEQRFFLGWAQVWGANMRPENARLM
                     ANTNEHPLPKFRTNGPLSNMELFAKAFGCKKGEAMVRENVCKIW"
     gene            881..1948
                     /locus_tag="VFR42_05135"
     CDS             881..1948
                     /locus_tag="VFR42_05135"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0368568.1"
                     /translation="MKTILVTGSSGLIGSEAAEHFDRQGQRVIGVDNNMRQVFFGTQG
                     DTTWNLQRLKRSTKTFEHANIDIRDRAALDNLFEANRFDMIVHCAAQPSHDKARDIPI
                     LDFEVNALGTVNLLEATRRHCREAVFILMSTNKVYGDSPNEIPLKELETRWEYARPED
                     YNGITESCRIDQTLHSLFGASKAAADLVAQEYGRYFGMNTCIFRGGCLTGPSHSGVEL
                     HGFLSYLVKVALTGGTYSVFGYKAKQVRDNIHSYDVVRAMEEFAASPRPGEVYNLGGG
                     RANSVSMLEAIAKIADMTGKKLNWKYVAENRKGDHICYISDLRKFESHYPNWKLTKNL
                     DAIFQEIIASQQAQQTQSVAS"
     gene            1997..3073
                     /locus_tag="VFR42_05140"
     CDS             1997..3073
                     /locus_tag="VFR42_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0368569.1"
                     /translation="MTILCLCREQHHWKLIPGYAEAFRRREIGFVCVDEAIPFDADLD
                     EVVRRSGATPSAIFHFESAYPLFPIGLEKSEVPTVCFHPDTYAFTQRRIRWSSVFDHA
                     VVFHPGFVEQFTKAGHPGAFLLPHAVRREFFDGPELPREFAVGWVGQTDGPFYRARAE
                     WLPKLARHFRTNDWSRSYSVQEVAEIYRRSKVVVNIGRDDFPQDANLRVFEVLGSGAL
                     LLTSLPSELQELGFHEGEHFIGYRDHREINGLVRKYLDDDPGRARIAAAARELVMSEH
                     TYDRRAEQLLSRLADKSKIGEAPARSWPDTSARLVAMDFYAAHGLTRCAATQFRRIAG
                     RGFRETIEAASLLSRASLRSLRGG"
     gene            3134..4807
                     /locus_tag="VFR42_05145"
     CDS             3134..4807
                     /locus_tag="VFR42_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0368570.1"
                     /translation="MLTLGINYSQMHDSSACMVRDGELLFAVAEERISRLKHDARFPQ
                     LAIQACLDFAKVTAAQVDEVCFGWQPAGQVFRHDLKLYATGQWPLSYMNLLNSTRHFA
                     SMWHQESGGAPFRRIFGSGKTRFRYVDHHLAHALSCYAYSGFDDAAVVVMDGRGAWEA
                     TSIWHGHAGKLGHVLTLNWPDSLGLFYAQFTGFLGFVPNSDEWKVMGLAPYGQAGVNL
                     SAYISPDDSPYRVHTKQLIGDSLGFAHFAPRLGPPREAESDISDHHKNIAFAVQDACE
                     VAVMSVVKLALAKTKAKNLCLAGGVALNSKANGKIVASGMIDQIFVQPAASDDGVALG
                     AALAPCLDGNGKLPDKPMRHGYWGPAFDDEAIEKALLTYKIRYTKLSDPATTAAQLLS
                     EGKILGWFQGRMEFGPRALGSRSIIADPRDPEMNAKVNNAVKFREWWRPFAPSLKKEA
                     APEYLESAYDSPFMILTAQVRHEKRSVIPSVTHVDGSARPQTVEREINPLYYRLIDEF
                     GKISGVPVIMNTSFNLRGEAIVHTPTDALRTFYSSGMDALVIGSFLVEK"
     gene            4804..5916
                     /locus_tag="VFR42_05150"
     CDS             4804..5916
                     /locus_tag="VFR42_05150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0368571.1"
                     /translation="MSAERVIALLGRRDEPTDAVEEYCRLLGSALHEHSFSMEIRRVP
                     WNEHGWPASLNALRLQGESWRDTWVLAQYTNLAWSSRGFPGRFLRVLRVLRKAGARIA
                     VVFHDAEPFSGTRLIDHFRRAVQLGVMRDAVAFSDCAVLTVPPARLSWLQNLQPTVSF
                     IPVGANLPQPLLEQDHDKLHDPPTIAVYSITGGPPGDRETDEIIRVVRHAAARVGKLR
                     LLVFGRHAEVRESLLQTGLQGCDVEVRVEGVIDADTLVERFAASDVLLFLRGTISSRR
                     GSAIAGIACGLPVIGLRGPETDAPITDAGVVLLEDNANDEDLSQQFAEALAKILSDDT
                     YRHGLAARSRSAQEDFFSWPAIARRYATMLQKNWQV"
     gene            complement(6172..>6417)
                     /locus_tag="VFR42_05155"
     CDS             complement(6172..>6417)
                     /locus_tag="VFR42_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368572.1"
                     /translation="VLERAYRLTTDEIVGAIERVLQTDVLIVENEQEVFTAMIALKEG
                     PGAFADAVIAALGVRMGCSWTLTFDQKALRLPGFKRP"
BASE COUNT         1375 a         1828 c         1872 g         1342 t
ORIGIN      
        1 cgttccagat caagatcggt tatccggaca agtggcgcga ctattcggcg ctcaccgtgg
       61 acaagggaag ctatctggca aacgagcggc gggcgactgc gtttgagaac gctcgcgact
      121 tgaacaagat cggcaaaccc gtggatcgca gtgaatggca aatgacgccg ccgacggtga
      181 acgcgtacta cgatgccgcc atgaacgaaa ttgtgtttcc tgcgggaatt ttgcaaccac
      241 ctttttacga tccgaaagcg gacgatgcgg tgaattacgg aggcatgggc gctgccatcg
      301 gtcacgaaat cactcacggc ttcgacgacg agggttcgca gttcgacgcg aaggggaccc
      361 tgcgcgactg gtggacgaaa gacgaccgca agaattttga cgagcgcgcc agctgcgtgc
      421 aaaaacagtt tgatggctac gaagtggagc ccggtctgca cgagaacggc aagctggtgc
      481 tgggagagag cattgcggac ctcggcggtc tggccatctc ctatgcagct tatgaaaaat
      541 caatcgaagg aaagcggccg ccgacgctgg gcggatttac gccggagcaa cgattcttcc
      601 tcggctgggc gcaagtgtgg ggcgccaaca tgcgtccgga aaatgcccgg ctgatggcga
      661 ataccaacga gcaccctctg cccaaattca ggacgaacgg gccgctctcc aacatggagc
      721 tttttgccaa agccttcgga tgcaaaaagg gcgaagccat ggtgcgggaa aacgtctgca
      781 agatctggta acgcaacgcg gtgtttttga tgaacaactt gggccgcggc gtgcgcgacg
      841 ccgcaaaatt gtctaaacta ttcctgccag ccacaacaag atgaaaacca tacttgtcac
      901 cggcagcagc ggtctgatcg gatccgaagc ggcggagcat tttgaccggc agggccagcg
      961 cgtcatcggc gtggacaata acatgcgcca ggtgtttttt ggcacacaag gcgatacgac
     1021 gtggaatctg cagcggctaa agcgctccac caaaacattc gagcacgcga acattgacat
     1081 tcgcgaccgc gcggcgctgg ataatttgtt cgaggccaat cggttcgaca tgatcgtgca
     1141 ttgcgccgcg cagccgtcgc acgacaaggc tcgcgatatt ccgattctcg acttcgaggt
     1201 taatgcgctc ggcacggtca atctcctgga agccacgcgc cggcactgcc gcgaagcggt
     1261 gttcatcttg atgagcacta acaaggttta cggcgactca cctaacgaaa tcccattgaa
     1321 ggaactggaa acgcggtggg aatatgcgcg gccggaagac tacaacggga ttaccgaatc
     1381 ttgccgcatc gatcagacgc tgcactcgct ttttggcgcg tccaaggccg cagcagattt
     1441 ggttgcgcag gaatacggcc gctatttcgg gatgaatacg tgcatttttc gcggcggctg
     1501 cctgaccggc ccatcgcatt ccggcgtgga gctgcacggc tttctgtcgt atttggtgaa
     1561 agtggcgttg accggcggga cgtattctgt gttcggctac aaagccaagc aggtccgcga
     1621 caacattcac agctacgacg tggtacgcgc gatggaagag ttcgcggcaa gtccgcggcc
     1681 gggagaagtt tataatctgg gcggcggccg ggccaacagc gtctccatgc tggaagcaat
     1741 tgcaaaaatc gccgacatga ccggaaagaa gctcaactgg aaatatgtgg ccgaaaaccg
     1801 caagggagat cacatttgtt atatctccga tctgcggaaa tttgagtcgc actatcccaa
     1861 ctggaagctc accaagaatc tcgacgctat cttccaagag attattgctt cgcagcaggc
     1921 ccagcaaact caaagcgttg cgagctaacg gtaccttttc ttggattcat gaatcctctc
     1981 gtcgcaccgt gtgcgcatga cgatcctttg cctctgccgc gaacagcacc actggaaatt
     2041 gattccgggc tatgccgaag cctttcgccg gcgcgaaatt ggttttgtgt gcgtggacga
     2101 agcgatcccg ttcgatgccg acctggacga agtcgtgcgg cgcagtggtg cgactccgtc
     2161 ggcgattttc cactttgaat cggcatatcc gctctttcct atcggcctgg aaaaatcaga
     2221 agttccaacg gtgtgcttcc atccggatac atacgccttc acccagcgca gaatccgctg
     2281 gtcttcggtt ttcgatcacg ccgtggtttt ccatccggga tttgtggagc aatttacaaa
     2341 agcgggccat cccggcgctt ttctgttgcc ccacgccgtg cggcgagaat tctttgacgg
     2401 ccccgaactg ccgcgcgaat ttgcagttgg ctgggtgggg caaaccgacg gacctttcta
     2461 ccgggcacgc gccgaatggc tcccaaaact cgcgcggcat tttcgtacta acgactggtc
     2521 gcgctcctat tccgtgcagg aagtggccga aatctatcgt cgttcaaagg tcgtcgtaaa
     2581 tatcgggcgc gacgattttc cacaggacgc caacctgcga gtgtttgagg tgctggggtc
     2641 gggtgcgctt ctgctgacgt cgcttccaag cgaattgcaa gagctgggat tccacgaggg
     2701 cgagcacttc attggttatc gcgaccatcg agaaatcaac ggcttggtgc ggaaatatct
     2761 ggacgacgac cccggccgcg cgcgaattgc tgccgccgcg cgcgaactcg tcatgagcga
     2821 acacacttac gatcgccgtg ccgagcagct gttgtcgcga ctcgcggaca aatcgaagat
     2881 aggggaggcg ccggcgcgct cgtggcccga caccagtgcc cgcctcgtgg caatggattt
     2941 ttatgccgca catggcctga cgcgctgcgc cgccacccaa tttcgtcgta ttgcggggcg
     3001 cggttttcgc gagacaatag aagcagcttc tctgctttct cgtgcctcgt tgcgctcctt
     3061 gcgaggcggg tgatgcggca atcgaacgtc gttttaggcg atatcgcgtg aaatcggaat
     3121 tgtgcgcaaa agcatgctga ctcttgggat caactattca cagatgcacg attcgtcggc
     3181 gtgtatggtt cgcgatggag aattgctgtt tgcggttgcc gaagagcgca tttcgcggct
     3241 gaaacatgat gcacgctttc cgcagctagc cattcaagct tgcctggatt tcgcaaaagt
     3301 cactgcggcg caagtggacg aagtatgttt cggctggcag ccggcaggcc aagtctttcg
     3361 ccacgacttg aagctctacg ccaccgggca atggccgctc tcttacatga atttgctgaa
     3421 ctccacgcgc cattttgcca gcatgtggca ccaggagtcc ggcggggcgc catttcgcag
     3481 gatctttggc tccggcaaga cgcgttttcg atacgtagat caccacctgg ctcacgcgct
     3541 aagttgctac gcgtattcgg gcttcgacga cgcggcagtc gtggtcatgg acggtcgcgg
     3601 cgcatgggaa gcgacttcga tttggcacgg ccacgctgga aaactcggcc acgttcttac
     3661 gctcaactgg cccgattcgc ttggcttgtt ttacgcgcag ttcaccggct ttttgggatt
     3721 tgtacccaac agcgacgaat ggaaagtgat ggggcttgct ccttacggcc aggccggagt
     3781 caatctcagc gcatacatca gtcccgacga ttcgccgtat cgtgtacata cgaagcagtt
     3841 gatcggggac agccttggct ttgcgcattt tgcgcctcgg ctcggccctc ctcgcgaagc
     3901 ggaatccgat atttccgatc atcacaaaaa tatcgccttc gccgtgcaag atgcctgtga
     3961 agtagccgtg atgtcggtgg tgaaactagc tctggcaaaa acaaaagcca aaaatctctg
     4021 cctggcgggt ggagttgcac tcaattccaa agcaaacgga aaaatcgtcg cgtccggaat
     4081 gattgatcag atctttgtgc agcctgcggc atcggatgat ggcgtggcgc tgggggccgc
     4141 gctcgctcca tgcctcgacg gaaacggtaa gctcccggac aagccgatgc gccacggcta
     4201 ttggggaccg gcgttcgacg acgaagctat cgaaaaagcg ctactgactt acaaaatccg
     4261 ctatacgaag ttgagcgatc cagcgacaac cgccgcgcaa ttgttgagcg aaggcaaaat
     4321 tcttggctgg ttccagggac gcatggaatt tggcccgcgt gcgcttggca gccgctccat
     4381 cattgccgat ccgcgcgacc cggaaatgaa cgccaaagta aacaacgccg tgaagttccg
     4441 ggaatggtgg cgcccgtttg caccttcttt aaaaaaagag gcggcgccgg agtatctgga
     4501 gtcggcgtac gattcgccgt ttatgattct gaccgcgcaa gtgcgtcacg aaaagcgcag
     4561 cgtcatcccg tcggtaacgc acgtagacgg tagcgcgcgc ccccagacgg tcgaacgcga
     4621 aatcaatccg ctctactacc gcttgatcga tgaattcggc aaaatctccg gcgtcccggt
     4681 catcatgaac acttctttta atttgcgcgg cgaagccatc gtccacacgc caaccgacgc
     4741 gctgcgaact ttctacagct cgggcatgga cgcgctggtc atcggcagct tcctagtgga
     4801 aaaatgagcg ccgagcgcgt cattgcactg ctggggcgca gggacgaacc gaccgacgcc
     4861 gtcgaagagt attgtcgact tcttggaagc gcgctgcacg aacattcgtt cagcatggaa
     4921 atccgccgcg ttccctggaa tgagcacggc tggccggcct cgctgaacgc ccttcgtctc
     4981 cagggagagt cctggcggga cacatgggtt ttggcgcagt acaccaacct ggcgtggtcg
     5041 tcgcggggct ttcccggccg tttccttcga gtgcttcgtg tgctgcgcaa agccggcgct
     5101 cgaatcgccg tcgtcttcca cgatgccgag ccattcagcg gaacgcgtct gatcgaccac
     5161 ttccgacgag cggttcagct tggcgtcatg cgtgatgctg ttgcgttttc agactgcgcc
     5221 gtgctgaccg tgccgcctgc tcgcttgagc tggttgcaga atcttcaacc aaccgtttct
     5281 ttcattccgg tcggagcaaa ccttccgcag ccgctgctgg aacaggatca cgacaaactt
     5341 cacgatccgc caacaatcgc ggtttacagc atcacaggcg gcccgccggg cgaccgcgaa
     5401 accgacgaga ttatccgcgt ggttcgccat gccgcggcaa gagtcggcaa actgcgcctg
     5461 ctggtttttg gccggcatgc ggaggtccgc gaaagtttgt tgcaaactgg actgcaaggt
     5521 tgcgacgtgg aagtccgcgt ggagggtgtc atcgacgctg acacgctcgt cgagcgcttc
     5581 gcggcttcgg acgttctgct ttttctccgc ggcacaattt cttcgcgacg aggcagcgcc
     5641 atagcgggca tcgcttgcgg actacccgtg ataggtctgc gcggcccgga aaccgatgca
     5701 cccatcaccg atgccggtgt cgttctgctc gaagacaacg caaatgacga agatttgagc
     5761 caacaattcg ccgaagcgct tgcgaagatt ctttccgatg acacctaccg tcacggcctg
     5821 gccgcccgca gccgctcggc acaagaagat tttttctcct ggccggccat cgcccgccgc
     5881 tacgccacaa tgcttcagaa aaactggcaa gtctaaatat ctgaaggaag caaccagctg
     5941 gcggaggagg gattcgactc tgcacagaat gagggcggca cgtgttggca tgcgttggcc
     6001 cgctaccgtc ggaagactga aaacaggtaa atttacgctc ttccgcataa cacaggctgt
     6061 ctccatccct gattgaggcc gttttgtagc acatgtagca tgacgggtgg ccgcgcctgc
     6121 gagaagccat agctacggag gcttcctcac gaaaggcgag acggcgtgaa tctatggacg
     6181 cttgaagcca gggagccgta gcgccttctg gtcgaatgtc agggtccagg aacagcccat
     6241 tctgacgccc agtgccgcga tcacggcgtc ggcgaacgcg cccggccctt ccttgagtgc
     6301 gatcatggcg gtgaaaactt cctgctcgtt ctccacaatg agcacgtcgg tctgcagcac
     6361 gcgttcgatc gccccgacga tttcatcggt tgttaacctg taagcacgct cgaggac
//
LOCUS       DASTPM010000291         3647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_10365, whole genome shotgun sequence.
ACCESSION   DASTPM010000291 DASTPM010000000
VERSION     DASTPM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10365"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /locus_tag="VFR42_05160"
     CDS             complement(<1..2538)
                     /locus_tag="VFR42_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0368573.1"
                     /translation="MFRPALIGCISLLALLVLLSAVSSTAQITTGEITGTVMDQSGAV
                     VGGATISAVCPDTNRTRSVSSGTAGEYRMTDMAACIYKVSVSAAGFKTTVRNVTVSVA
                     QTTNADFQLQLGERTETVTVEAATPLVEYSPGVNNEVDTKSIVDLPTEGRDFKSILAL
                     TPGVQRTPGGGFLDVSVSGQRTTSNNYMIDGMPNNDRFYGSEVVGQPGVLGVPASVLG
                     NDSISEYTVQQLPTAENGVKGGAAVNVTLKSGTNQFHGTAFYFGDYDWLNAKNYFSSG
                     TLPYHNHNYGGTVGGPIVKDRTFFFFAFEGQRNKALEPYDVLIPTQGDLAAALSDFAS
                     PTVNAHGLPLNSAGLNMLKFYPCSDGAGGVVSCTPSAANAVVDTGGFTQTISSPNTDK
                     MSSYIVRMDHKMNDKMQLSGRYTFADSVQSAPLGGYTLPPAPGSGLGPDAFNSVAPTR
                     VQLAGATWTYSMSPNKILDVRFNWSRYAQILAPNNKVNPLDLGIDTGPLNPADFGVPP
                     VYASSVTYGNPGGIQGYPLSTRPTQNYNASAKISWIKGKHTFKFGGSYEYASTFSLRN
                     RARTGLVSSQFDFETLIDQLLLGRFDQAGRSFGDTSRHLYQPSMGLFFQDEWKVTPRV
                     TISYGLRWDVNGALGDADKNASNFFPCAEPEPTVCAPKGFPNGLVQVQEGFPRLYNLD
                     LKNLGPRAGLAWDVFGNGKTALRVGYAMAYDVANFAAISAPYTFQGARAGAFTNSNLG
                     VFSVNAVGNGCTGFLNPDPDVGCEPGLSTLFEAFGPNTCYNPATNTSDLNPLTGTKGY
                     DWVCIGPQAGSPNPSVPFQTFGASPGATPPFNIFGTVPDLKTPRIQYY"
     gene            complement(2850..>3647)
                     /locus_tag="VFR42_05165"
     CDS             complement(2850..>3647)
                     /locus_tag="VFR42_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368574.1"
                     /translation="GFRQRNSQVPTYAPGLFRASSDQDVRNRITFSGGWDLPFDRAWN
                     SGPKRLTQGWSVFPIVTWRSGLPIDIFARLPDRFDPGAEGPSGAGDPFSVHANIVGST
                     ATFDPKKAQTFVNPNDPTDVRTGNFYFSPFSFSNAQCGDANHPLATCIPGPDILPANS
                     QVVADPALATYGTYPRNFLRGPGYINFDLGFAKTTAITERLKLEFRAEFFNIFNHANF
                     LNPNVINNGDGTFAGGAPGTNINSGSFGQITGTYDPRIIQFGLRLAF"
BASE COUNT          822 a          995 c         1076 g          754 t
ORIGIN      
        1 gtaatactgg attcttggcg tctttaaatc cggcacggtt ccaaaaatgt taaagggcgg
       61 cgtggcccca ggactggcgc caaaagtttg gaacggtacg gatgggttgg gactcccagc
      121 ttggggaccg atgcaaaccc agtcataccc ctttgtacca gtgagagggt tcagatctga
      181 agtgttcgtc gccgggttat agcaagtatt cggcccaaac gcttcaaaga gcgttgagag
      241 gcccggttca catccgacgt ccgggtccgg gttgaggaat cccgtgcagc cgtttcctac
      301 ggcgttaacc gaaaacacgc ccaggttgga gttcgtgaat gccccggctc tggcaccctg
      361 gaaagtgtaa ggcgcactga tcgcggcaaa gttcgccacg tcgtaggcca tagcataacc
      421 aactcggaga gcggtcttgc cgttgccgaa gacatcccaa gccaatcctg cgcgtggacc
      481 gagattctta aggtccaaat tatacaagcg ggggaagccc tcctgaacct gaaccaaacc
      541 gttcgggaac ccttttggcg cacagaccgt aggctcaggc tcagcgcatg ggaagaaatt
      601 ggaagcattc ttgtcggcgt cacccaaggc gccattgacg tcccagcgca agccataact
      661 gatcgtcacg cgcggagtga ctttccattc gtcttggaaa aacaacccca tcgaaggctg
      721 gtagagatgt cgcgaagtat cgccaaagga acggccagcc tggtcgaagc ggccgaggag
      781 caactggtct atcagcgttt caaaatcaaa ttgcgagcta accaaaccgg tccgtgcacg
      841 atttcgcagg ctgaacgtgg aggcgtattc atagctgccg ccaaatttga aggtgtgctt
      901 gcccttgatc caggagatct tggccgaagc gttataattt tgcgtgggcc gggtgctgag
      961 cgggtaacct tgtatgccac cgggatttcc ataggtgacc gaactagcat agaccggagg
     1021 cacaccgaaa tccgcggggt tcagagggcc ggtgtcaatg cccaggtcca agggattaac
     1081 cttgttattt ggcgccagaa tctgtgcgta acgcgaccag ttgaatcgca cgtcgagaat
     1141 tttgttgggg gacatgctat aggtccaggt ggcgccggcc aattgcacgc gcgtcggagc
     1201 gacggagtta aatgcatccg gtccgagacc gctgccaggc gccggcggaa gggtatatcc
     1261 gccgagcggt gcggattgaa cgctgtccgc gaacgtatac cggcccgaca gctgcatctt
     1321 gtcgttcatc ttatggtcca tcctgacgat gtagctgctc attttgtcgg tattcgggct
     1381 ggaaatggtc tgcgtaaacc cacccgtgtc tactactgcg ttagccgcgg agggtgtgca
     1441 ggaaacaacc ccgcccgcgc catccgaaca ggggtaaaac ttcagcatgt tcagtcctgc
     1501 gctattgaga ggcaggccat gagcgttgac agtaggactg gcgaagtccg acagagccgc
     1561 ggccagatcg ccttgggtgg ggataagtac atcataaggc tcgagcgctt tgttgcgctg
     1621 cccttcaaac gcgaagaaga aaaacgtgcg gtccttgacg atcggtccgc cgaccgtgcc
     1681 gccataattg tgattgtggt agggcagcgt tcctgaggag aagtagttct tggcgttcaa
     1741 ccagtcgtag tctccaaaat agaaggccgt tccgtggaac tggttagtac cgctcttgag
     1801 ggtaacgttg actgcggctc cacccttaac cccattttct gccgtcggaa gctgctggac
     1861 cgtatactcc gagatcgaat cgttgcccag aacggaagcg ggaaccccca atacgccggg
     1921 ctgcccgacg acctcgctgc cgtagaagcg gtcgttgtta ggcatgccat caatcatgta
     1981 attgttggag gtggtgcgct ggccgcttac gctaacgtcc aggaacccgc caccgggcgt
     2041 gcgctgcact ccgggagtca gggccaagat tgacttaaag tcacggccct cggtcggcaa
     2101 atccacgata gacttcgtgt ccacctcgtt gttcacgccc ggggagtact ccaccaatgg
     2161 cgtggccgct tccactgtga cggtttccgt gcgctcgcca agctgaagct ggaaatcggc
     2221 attggtcgtc tgggcgacgc tgaccgttac gttgcgtaca gtagtcttga atcctgcggc
     2281 ggaaacggaa actttgtaga tacaggccgc catgtccgtc atccggtatt cgccagcagt
     2341 tccggaggaa accgagcgcg ttcggttcgt gtcgggacaa accgcggaga tagttgcgcc
     2401 acccacgact gcgccgcttt gatccatcac cgtcccggtg atttctccgg tggtgatttg
     2461 agcagtcgag gaaacagccg acagcagaac aagaagcgcg aggagagaaa tacagccaat
     2521 aagtgcagga cgaaacatga ggaccacccc ctagggcgct ttaggccgtc gtacgccaat
     2581 tacaattggg agtgttttag gacaatttga cctaaagtgt caagcaaagt tcctctaatt
     2641 catacgcacc tgacacccaa tacaggacgc atttgtcggc ctcgacaagt aatgtccaaa
     2701 aaaacagaca cctcggaggt gtttcgctcc agggccacct tgtgtgttcg tttgttgctg
     2761 cgttgaaact cgtcgcgcag cgcgacgagg agagccaacc gggacgaaaa gctttggctt
     2821 tccgtccctg gccgacgcga agaacacggc taaaaggcaa gccgcaagcc gaactggata
     2881 atgcgagggt cgtaagttcc agtgatttga ccgaaactcc cgctgttgat gttcgtgccg
     2941 ggcgcgccgc cggcgaaagt gccatcacca ttgttgatga cgttgggatt caggaagttt
     3001 gcatggttga agatgttgaa gaactcggcg cggaattcca gcttcaacct ctcagtgatg
     3061 gcggtggtct tcgcaaaccc caggtcaaag ttaatgtatc caggtccgcg caagaaattc
     3121 cgcgggtacg tgccatatgt ggcgagtgca ggatcagcga cgacctgcga attggccggc
     3181 aagatatcgg gaccaggaat gcacgtcgca agcgggtggt ttgcgtcgcc gcactgcgca
     3241 ttgctgaagc tgaacggact aaaatagaag ttgccggttc tcacgtcggt cgggtcgttc
     3301 ggattcacga acgtctgagc tttcttcgga tcaaaagtcg ccgtcgatcc cacgatgttc
     3361 gcgtggacac taaacggatc tcccgctccc gaaggacctt ctgcgcccgg atcgaaccga
     3421 tccggcaacc gcgcgaaaat gtcgatcggc agccccgaac gccaggtgac aatcggaaag
     3481 acactccagc cctgcgtcag ccgcttcggg ccggaattcc atgcccggtc gaagggcaaa
     3541 tcccatccac cgctgaaggt gatgcggttg cggacgtcct ggtcgctgga agcgcggaaa
     3601 aggcccggcg catacgtggg aacctgcgag tttctttgcc ggaagcc
//
LOCUS       DASTPM010000292         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_529493, whole genome shotgun sequence.
ACCESSION   DASTPM010000292 DASTPM010000000
VERSION     DASTPM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529493"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFR42_05170"
     CDS             <1..451
                     /locus_tag="VFR42_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368575.1"
                     /translation="ERLESAGIPFRDKALGEDQVAPTADPLPISWKPRFGFEVSVLAS
                     DFTAANVIAEELLGQEPADLELPAQDQGSSPEPPLVSETELNPTAEVWSGMDDRIAQF
                     LTAAMQENEIPIHLETSGGLTRIFVSPANEVRAREIVREIVQAAPPE"
     gene            530..1393
                     /locus_tag="VFR42_05175"
     CDS             530..1393
                     /locus_tag="VFR42_05175"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEU0368576.1"
                     /translation="MSIDSIRTVAVLGAGTMGNGIAHVFARAGYKVLLRDVEERYLDR
                     ALDTISKNLDREVKKGKLAEAEKATVLGHLVPVTDMSTIANADFVVEAVPEKLELKRQ
                     VLTEADRLLRDDVIIASNTSSIAITTLAALTKRPDRFVGMHFMNPVPVMVLVEVIRAL
                     QTSEATFNATMQLAERLGKKPVAVNDAPGFVSNRVLMPLINEAAYCVMEGVATPEAVD
                     AVMKTGMNHPMGPLELADFIGLDVCVDIMHVLHEGLGDPKYRACPLLKKYVSAGWLGR
                     KAGRGFYSYGS"
     gene            1714..2838
                     /gene="recA"
                     /locus_tag="VFR42_05180"
     CDS             1714..2838
                     /gene="recA"
                     /locus_tag="VFR42_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU0368577.1"
                     /translation="MADEKTKQLETAIAVIEKSYGKGSIMRLGSRDVLVPVSVIPTGC
                     LSLDAALGVGGFPRGRVVEVYGPESGGKTTMTLHVIAEAQKLGGTAAFIDAEHALDPI
                     YARKLGVDVDNLLVSQPDNGEQALEIAQELIRSNAVDIVVVDSVAALVPKAELEGDMG
                     DPQMGLQARLMSQALRKLTAIVSKSRTCLIFINQIREKIGVMFGNPETTTGGRALKFY
                     ASIRLDIRRIQAIKEGDRVIGSRTRGKVVKNKVAAPFREAEFDILYGEGISREGDLID
                     LGIDKGLLEKSGTWISFGGERMGQGRENARVFLKENKDIRDKLETALRKKLEIALPGN
                     SGATSSGVNGHAATHPQTDKVPQKAAAAAASAADARRPSR"
BASE COUNT          648 a          809 c          924 g          631 t
ORIGIN      
        1 ggagaggctg gagtccgcgg ggattccctt tcgtgataag gctttgggcg aggaccaagt
       61 cgcccccacg gctgatcctt tgccgatctc ttggaaaccg cgctttggat ttgaggtgtc
      121 tgtactggcg agcgacttca cggcggcgaa cgtcatcgcg gaggagttgc tcgggcaaga
      181 acccgccgac ctggaattgc cggcgcagga tcaggggtct tctccggagc cgccgcttgt
      241 ttctgaaacc gaactaaatc cgaccgctga ggtttggtcc ggaatggacg atcgaatcgc
      301 gcaatttttg actgccgcta tgcaggagaa cgaaatcccc attcatctcg agacatccgg
      361 cggtctaacg cggatatttg tgtcgccggc gaatgaagtg cgcgctcgcg aaatcgttcg
      421 cgaaatcgtc caggccgcac cccccgaata agggagcgca attcagagcg ctttacgttt
      481 tccttctggc gcattcaact gtagactttc ggcgatcttg gagagctttg tgtcgatcga
      541 ttcgattaga acggttgcgg tgcttggcgc tgggaccatg ggaaacggca tcgcgcacgt
      601 gttcgcgcgg gcgggatata aggtgctgct gcgcgacgtt gaggaacggt atctcgatcg
      661 ggcgctggac actatttcaa agaatctcga ccgcgaggtg aagaagggca aattggcgga
      721 ggcggaaaag gcgaccgtgc ttgggcacct tgttccggtc actgatatgt cgaccatcgc
      781 caacgctgat tttgtggtcg aggccgtgcc ggaaaagctg gagctgaagc gtcaggttct
      841 caccgaggct gatcggctgc ttcgagacga tgtgatcatc gcaagtaata cctcgtccat
      901 tgcgattacg acgctggccg cgcttaccaa gcggccggat cgctttgtgg gcatgcactt
      961 catgaatccc gttccggtca tggtgctggt ggaagtaatc cgggcgttgc aaaccagcga
     1021 ggctaccttt aacgccacga tgcaactcgc agagagactc ggcaaaaagc ctgtggcggt
     1081 aaatgatgcc cccggtttcg tttccaaccg cgtgctcatg ccgctgataa atgaggccgc
     1141 ctattgcgtg atggaaggcg tggccactcc ggaggccgtt gacgcggtga tgaaaacggg
     1201 catgaaccat cctatgggcc cactggagtt ggcggacttt atcggtcttg atgtttgcgt
     1261 cgatatcatg cacgttttgc acgagggtct cggcgacccc aaataccgcg cgtgtccctt
     1321 gctgaagaaa tacgtgtccg cgggctggct gggccgcaag gccggccgtg ggttctatag
     1381 ctacggctca tagctggagg agctacggct catagctgga ggcctagaac agctcacgaa
     1441 agtatttgct ttttgttcgc ctacgtgcta cggtccttct gcgcggtttt caacaggagg
     1501 ctttcattgg gctcttgctc caagacggaa acctgtcatt ctgacggcct ccatgaaatc
     1561 gcacgagaaa agcgtggcag cggcataaac agaatggtaa cctaccgctt ccattatgta
     1621 atccggcgac ggctcggcgc tttcccggcg ccaagacgtg cctaacaagg tcgtgcagca
     1681 aggatttcct aactttcctt accgaggtgt gaaatggcag acgagaagac gaaacagctg
     1741 gaaacggcga tagcggtaat tgaaaagtcc tacggcaagg gctctatcat gcgtctgggg
     1801 agccgcgatg tgctcgtgcc ggttagcgtt atacccaccg gatgcctttc gctcgatgcg
     1861 gctctggggg taggcgggtt tccgcgcggg cgcgttgtcg aagtctatgg cccggaatcg
     1921 ggcggaaaaa ccactatgac gctgcacgtc attgccgaag cgcaaaagct tggcggcaca
     1981 gccgcgttta tcgatgccga gcatgcgctc gaccccattt acgcacgcaa gcttggcgta
     2041 gatgtcgaca atcttcttgt ttcccagccg gataacggcg aacaggcgct ggagatcgcg
     2101 caggagctga tccgctcgaa tgccgtcgat atcgtggttg tggattcggt cgctgcgctg
     2161 gtgcccaagg ccgagcttga gggcgatatg ggcgaccctc aaatgggttt gcaagcgcgg
     2221 ctgatgtcgc aagcattgcg caagctcacc gccatcgtct ccaagtcgcg cacgtgcctg
     2281 atattcatca atcaaatccg cgaaaaaata ggggtcatgt tcggcaatcc ggaaacaaca
     2341 actgggggcc gcgcgctgaa gttttacgca tccattcgcc tggacatacg ccgcattcaa
     2401 gctatcaagg aaggcgaccg ggtgattggc tcgcggactc gcggcaaagt ggtgaagaac
     2461 aaagtggcgg cgccattccg cgaagccgaa ttcgacattc tctatggcga aggcatctcc
     2521 cgcgaaggcg acttgatcga cctcggcata gacaagggct tgctggaaaa gagcggcaca
     2581 tggatcagtt ttggcggcga acgcatgggc caaggccggg aaaacgcccg cgttttcctt
     2641 aaggagaaca aagatatccg tgacaagctg gaaaccgcgt tgcgcaagaa gctggaaatc
     2701 gcgctgcctg gcaattcggg cgcaacgtcc agcggcgtca acggccacgc cgcaacgcat
     2761 ccccagaccg acaaagtgcc gcagaaagcc gccgcagctg cagcgtcagc agctgatgcc
     2821 cggcgcccat ctcgttagct aagggctgat tgcgctgtga tggctgcggt gagcacgaac
     2881 aggctcgggt tgccagcgct acagcatcat tgcagatgtg gctacaaatt tccgcgtatg
     2941 gtcactgacc ttgcctaatg cgagtatcgc gttgcgggtt actaagctcc gctagaacga
     3001 agtagaaggg gg
//
LOCUS       DASTPM010000293         5277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_183442, whole genome shotgun sequence.
ACCESSION   DASTPM010000293 DASTPM010000000
VERSION     DASTPM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5277
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183442"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            776..2611
                     /gene="typA"
                     /locus_tag="VFR42_05185"
     CDS             776..2611
                     /gene="typA"
                     /locus_tag="VFR42_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU0368578.1"
                     /translation="MSQNIRNIAIIAHVDHGKTTLVDAMLKQSGIFRANQEVADRVMD
                     SNDLERERGITILAKNTALFFHDTKINIVDTPGHSDFGGEVERALRMVDGVVMLVDAS
                     EGPLPQTRYVLQKALAAKLPPVVVLNKIDRTDARPKEVLDEIYDLFIDLDATEDQLDF
                     PIIYTNARDGVAHRTPGDGSTDLLPLFETIVARIPAPAGAPAATLQLQVMNLDYSEFL
                     GRIAIARVFNGTLKRGTEVGISKLDGKLQPTRITKLFTFRGLERDEAEEVHAGDVVAI
                     AGVEGIQIGESITDLSDPQPLEPLIIDEPTLAMIFTVNSSPLSGREGSYLTSRDLRDR
                     LQRELLTNVSIRVEDTDTPESFRVLGRGELQLAILIETMRREGFELMVGKPEIVVRNE
                     NGKKLEPVERLVVDIPENFIGIILETLGSRRGEMLKMNNHGSGRVRMDFKIPSRGLIG
                     LRSQLLTDTRGTALLHSIFDGWVEYGGEMALRPTGALVADRAGVSTAYALWNIQERGE
                     LFVGPAIDVYEGMIVGENSREQDMEVNVTREKKLTNMRSSSADEAIRLIPHRELSLEQ
                     AIEFIADDEYVEITPKSIRLRKKVLQYNKRPRRWQEIRASQEAAG"
     gene            complement(2631..3722)
                     /locus_tag="VFR42_05190"
     CDS             complement(2631..3722)
                     /locus_tag="VFR42_05190"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0368579.1"
                     /translation="MKRLAFPWISVVALAITFATDGRAQNAPKYAAEEGDYVAKDFRF
                     RSGETLPELKLHYRTLGKPVPDAKGHITNAILVLHGTGGSGQQFLSPQFAEELYGAGQ
                     PLDTSKYFVILPDGIGHGKSSKPSDGMHAHFPSYDYDDMVAAHHLLLTKGLSVDHLRL
                     VFGTSMGCMHSFVWGETFPDFMDALMPLACLPVQIAGRNRIWRKMVMDGIRNDPEWNG
                     GEYKEEPKQAVQIALDFLIIAGSAPLPMQKSFPTRDAADKFLEGYFQSHASSLDVNDL
                     LYQLNASRNYDPSPQLEKITARVMWVNSGDDFINPPELGIAQREAKHLKNGNFILLPA
                     SEQTHGHGSHTWAVLWKKQLSELLEQTAK"
     gene            complement(3760..5127)
                     /locus_tag="VFR42_05195"
     CDS             complement(3760..5127)
                     /locus_tag="VFR42_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0368580.1"
                     /translation="MNRHRFDYLRTALGLLLIAWACAANARAQQGNQPLLVMISVDGM
                     RPDYITEADSHGAKVPNLRRFMKEGAYADGVQGIVPTVTYPSHTTLVTGVWPAKHGIY
                     SNSTFDPLQKNADGWYWYNEDLHASTLWDAVAKTGRKTASVQWPVTVGARIDWLIPEI
                     WRAGTAEDGKLLRALCTPGLLAEISPELGMYRGGFDSSPEADALRGKYAEWILQKKHP
                     GILTLHLLGLDHEEHDSGPFSPQSIAILEQLDAVIGSLRAVAEKVAPGHAYIAVVSDH
                     GFAKVDSQLNLFAAFLEAKLFTLNDKGKIGDWKAMPWETGGSAAIVLKDPNDQATLSA
                     VRDLLAKLAADPANGIDRLLNADELHERGGYPIASFFVGLKPGWKTVSSLTGPVVSKT
                     AKTLGTHGELPDLPDLRASFFVVGAGVPAGKDLGLIDMRSVAPTLAHLIGAPLPSADG
                     KNLLP"
BASE COUNT         1147 a         1576 c         1444 g         1110 t
ORIGIN      
        1 ttctgcagca aaagcagtac agcctcaaat cttcatttga cagtgcgaaa tcatctccag
       61 aaggcagcaa tagaatcggc tggtgccatc ggagcggacc tgctcattac tggcatcgtc
      121 ggaaacgagc agcagggctc aattccgacg ataaccgtct tgcgagtttc caccggggag
      181 aaagtggagc aactgaaact tcgattccct cgactgaccc gtccaccagc ctggcaccag
      241 cctggcaaac cagcttgtgc atccgcttgg cccgatctac cttatggcca gcacggtgtt
      301 agcgctccgt cacctattgt ccgacgcctc tctgcttcga ccaagcccga aaggaaaaga
      361 tccaaggcaa ggtagtggcg acccatatca tcaataccgt cgggcaaccg gaaaaaatct
      421 ggctgaccaa gggattgccc cgcggcttgg cgcgcgactg gaaatggcaa gcctatacca
      481 gtgatcgttc ccgtcgatgt cgccttccat ttgtgacccg gggttctcta tgaccttggc
      541 ggtcatctac ctgtctcctt aactggctgg aatgctccgc ctttgataaa ctattcctgc
      601 aacgccacac ctgagaaatt cctgatctgg tccgtccgga tcgatacgag taaggaagga
      661 ttcgcaaagg ctgctggttg ttccagcagc tatttttgtc tccgaccgct aagcggtccc
      721 cgcaattcga ccgcccaagc ggcgaagcgg ttcgaagaga cccgagaagc aaattatgag
      781 ccagaatatt cgcaacatcg ccattattgc ccacgtggac cacggcaaga ccacgctggt
      841 ggatgccatg ctcaaacaga gcggtatttt tcgcgccaac caggaagtcg ccgaccgcgt
      901 tatggactct aatgacctgg agcgcgagcg cggtatcacc atcctggcca aaaacaccgc
      961 gctctttttc cacgacacca agatcaacat tgtggacacg cccggccaca gcgatttcgg
     1021 cggcgaagtg gaacgcgcgc tgcgcatggt agacggcgtg gtgatgctgg tggacgccag
     1081 cgaagggccg ctgccacaga cgcgctatgt tctgcagaaa gcgctggcgg cgaagctgcc
     1141 tcccgtcgta gtgctcaata aaattgaccg caccgacgcc cgccccaagg aagtgctgga
     1201 cgaaatttac gacctcttca tcgacctgga cgcgacggaa gaccagctcg acttcccgat
     1261 catttatacg aatgcccggg acggagttgc gcatcgcacg cccggcgatg gttcgacgga
     1321 cttgctgccg ttgtttgaaa ccatcgttgc gcgcattccc gctcccgccg gcgctcccgc
     1381 ggcgacactg caactgcagg tgatgaacct cgactacagc gaatttttgg gccgcattgc
     1441 gatcgcgcgc gtcttcaacg gaacattgaa gcgcggcacg gaagtcggca tcagcaagct
     1501 ggatggcaag ctgcagccca ccagaattac caaacttttt acgttccgcg ggctcgagcg
     1561 cgacgaagcg gaagaggtgc acgcaggcga tgtcgtcgcc attgcgggag tcgaaggcat
     1621 tcagatcggc gaaagcatca ccgatttgtc cgatccgcaa ccgctcgaac cgctcatcat
     1681 cgacgagccg acgctggcga tgatttttac ggtcaactcc agcccgcttt ccggtcgcga
     1741 aggcagctat ctaacgtcgc gcgatttgcg cgaccggcta cagcgcgaat tgctaaccaa
     1801 cgtttccatc cgcgtggaag ataccgatac gcccgagtca ttccgcgtgc tgggccgcgg
     1861 cgaattgcaa ctagccattt tgattgaaac tatgcgccgc gaaggctttg agctgatggt
     1921 cggcaagccg gaaatcgtgg tgcgcaacga aaacggcaag aagctggagc ccgtggaacg
     1981 gctcgtggtg gacattccgg aaaacttcat cggcatcatt ctggaaacgc tgggcagccg
     2041 gcgcggcgaa atgctcaaga tgaataatca tggctccggc cgcgtgcgta tggacttcaa
     2101 gattccttcg cgcggattga tcggcttgcg cagccagttg ctgacggaca ctcgcggcac
     2161 ggcgctgcta cactccatat ttgatggatg ggtcgaatat ggcggcgaaa tggcgctgcg
     2221 tccgacaggc gcattggtgg ctgaccgcgc cggggtttcc actgcttatg cgttatggaa
     2281 cattcaggag cgcggcgaac tttttgttgg cccggcaatc gacgtgtacg aaggcatgat
     2341 cgtcggcgaa aattcgcgtg agcaggacat ggaagtaaac gttacgcgcg aaaagaagct
     2401 gaccaacatg cgttcgtcca gcgcggacga agcgatccgc ttgattccgc accgcgaatt
     2461 aagcctcgaa caagccatcg agttcatcgc cgacgacgag tacgtcgaga tcactcccaa
     2521 gtccattcgc ttgcgcaaga aagtattgca gtacaacaag cgcccgcgcc gctggcagga
     2581 gatacgcgcc agccaggaag ccgcgggcta agcagatttt ttctgaacac ttacttcgcg
     2641 gtttgctcca gcaactcgga cagctgcttc ttccacagca ccgcccaggt gtgtgaaccg
     2701 tgcccatgcg tttgttcgct agcgggaagc aggatgaaat tcccgttttt tagatgtttc
     2761 gcttcgcgct gcgcgatgcc cagctccggc ggattgataa agtcgtcccc cgaattaacc
     2821 cacatgacac gcgccgtgat cttttccagt tgcggcgacg gatcgtagtt gcgcgacgca
     2881 ttcagctgat agagcagatc gttcacatcc agactgctcg cgtgcgactg gaagtagccc
     2941 tccagaaact tgtcggcagc atctcgtgtt ggaaaagact tttgcatcgg aagaggcgcg
     3001 ctgcccgcaa tgatcagaaa gtccagcgcg atctgaacgg cttgcttcgg ctcttccttg
     3061 tattcaccgc cgttccactc cgggtcgttg cgaattccgt ccatcaccat cttgcgccag
     3121 atccggttgc ggcccgcgat ctgcaccggc aggcacgcca agggcatcaa cgcgtccatg
     3181 aaatctggaa acgtctctcc ccaaacgaaa gaatgcatgc agcccatcga cgtgccgaaa
     3241 accagccgca gatggtcgac gctgagccct ttcgtcagca gcagatgatg cgcggcgacc
     3301 atatcgtcgt agtcgtacga aggaaaatgg gcgtgcatgc cgtcgctggg cttcgacgac
     3361 tttccgtgtc cgatgccatc cggcaaaatc acgaagtatt tgctggtatc gagaggctgg
     3421 ccggcaccgt acagctcctc ggcaaattgc ggcgacaaaa attgctggcc agagccgccg
     3481 gtgccgtgaa ggaccaatat ggcatttgtt atgtggcctt ttgcatcggg gactggcttg
     3541 ccaagcgtgc gatagtggag cttaagttcg ggcaaagttt cgccggagcg gaacctgaag
     3601 tccttcgcaa cgtaatcgcc ttcttcggct gcatacttgg gcgcgttttg tgcgcggccg
     3661 tccgtggcga atgtaatcgc tagcgcgacc accgaaatcc agggaaacgc caagcgtttc
     3721 atattctgct ctcccatgtt gtgcgcgatt ccaatgcttt tacggcaaca aattctttcc
     3781 gtcggcgctg ggtaacggcg ctccaatcag atgcgccagc gtcggagcga cgctgcgcat
     3841 gtcgatcagg cccagatcct tcccggccgg tacgccggcc cccaccacga agaacgacgc
     3901 gcgcagatcg ggcaaatccg gcagctcgcc atgtgtgcca agcgtctttg cggttttgct
     3961 gaccaccgga ccggttagcg acgaaactgt cttccagccc ggcttcaatc ccacaaagaa
     4021 agaagcaatc ggatatccgc cgcgttcatg caattcgtca gcgttcagca agcggtcgat
     4081 gccgttggcc ggatcggccg ccagcttcgc caacaaatcc cgcacagctg acagcgtcgc
     4141 ttgatcgttt gggtctttca gcacaatcgc tgccgagccg ccggtttccc acggcatggc
     4201 cttccagtcg ccgatttttc ctttgtcgtt cagcgtaaaa agtttcgctt ctagaaacgc
     4261 cgcaaacaaa ttgagctgcg aatccacctt tgcgaatccg tgatcggaaa ccaccgcgat
     4321 gtaggcatgg ccgggcgcca ctttttccgc gacggctcgc aacgaaccaa tgacggcgtc
     4381 aagttgttcc agtattgcga tcgactgcgg gctgaaaggc ccggagtcgt gctcctcgtg
     4441 atcgaggccg agcagatgca gcgtcagaat tcctggatgt tttttctgta aaatccactc
     4501 tgcatacttg ccgcgcagcg catccgcttc cggcgaagaa tcgaatcccc ctcgatacat
     4561 gcccagttcc ggactaattt ccgccagcag tcccggtgta cacaacgcgc gcagcagctt
     4621 tccatcctcc gcagtgccgg cgcgccagat ttccggaatc agccagtcga tccgtgcgcc
     4681 aaccgttacc ggccactgca cgctggccgt tttcctgcct gttttggcca ccgcatccca
     4741 caaagtcgat gcgtgcaaat cctcgttgta ccaataccag ccatctgcat tcttctgcag
     4801 cggatcgaac gtcgagttcg aatagattcc gtgcttcgcc ggccatactc ccgtcaccag
     4861 tgtggtgtga ctcggatagg tgaccgtcgg cacgatgcct tgcaccccat cggcgtacgc
     4921 gccctctttc atgaatcgcc gcaggttcgg cactttcgct ccatgcgaat cggcttccgt
     4981 gatgtaatcg ggccgcatgc cgtccacgga aatcatcacc agcaatggtt gattgccttg
     5041 ctgcgcgcgg gcgttggctg cgcaagccca tgcaatgagc aacaatccaa gcgcagttct
     5101 gagataatca aaacggtggc ggttcatcct tgggattctc ctgatgaggc agctatctta
     5161 cagcgcgaag atccgttttt cgcggtggca aagaaaaatc acacattatc gttgaaagcg
     5221 acacagcgct cctgttgttg caaatgtcgc gctttcactg gcaaaaccct gcatctc
//
LOCUS       DASTPM010000294         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_875346, whole genome shotgun sequence.
ACCESSION   DASTPM010000294 DASTPM010000000
VERSION     DASTPM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875346"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VFR42_05200"
     CDS             <1..1583
                     /locus_tag="VFR42_05200"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HEU0368581.1"
                     /translation="SSQGTSGKSTNTSCVFNDITGTTSKDGLTPTLNNSVPCTGASTN
                     CSKTSSGGFGVLTSAGSPAFTAGLGYDLATGLGTPNVANLINAWAAVSGTGSNATTVT
                     LAPATINGTAGSNFTLSGTVTKSSGSGTPTGVVVFENAATGTPAGNIPLLNLLFGGIS
                     NDPATLNSGGSYSVSTGLLPAGTYSLKAHYVGDATFAPSDSAPISVTLSKQASTVVVS
                     FVTANGTITTNSQSVQYGSPYILRVDVNGTSGSCTDSNGNVVRICPTGTITLKDNGSA
                     LNDFPNAQNANATNVAKLNNRGFAEDQPIQLNVGAHPITASYTPDPTSSYLVPTTGSN
                     TLSVTITQATTSVVVTPSVTSVVSGGSITLTATVNSQSNSAQGPTGTVQFKNGSTNLG
                     AAVTCTPKGADSNGSTMVGALCTAQLATTLSALPPGFFVEQRPRNTPFVIVTSLAALL
                     AMLSFVLALKLGGRRRRFAYAGAMFALIAASALAGCNGGSPSGGGGGGGSARTISAAY
                     SGDTNYATSTGSTSVTVE"
     gene            complement(1917..2300)
                     /locus_tag="VFR42_05205"
     CDS             complement(1917..2300)
                     /locus_tag="VFR42_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0368582.1"
                     /translation="MKPPTPPHKHVLGTPVKPGLTRPLTSEERRRAQRVLLRVPVRIS
                     VAGKKQPLEGATHTVSATGALIVLSEALQQGTKLTLENATTQKKVEAHVARPPQFSPD
                     GSLVAIEFASPSPNFWNVFFPPVIN"
     gene            complement(2297..2653)
                     /locus_tag="VFR42_05210"
     CDS             complement(2297..2653)
                     /locus_tag="VFR42_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368583.1"
                     /translation="MQVKTPEQKRVGLREVGNRYSEKRRGHRMNSRVRVRAEWEAEGK
                     RVVAESFTRVVNPYGCMVIFKDALDLEHRLSLTNLATGSTNAAVVVWRGNPRPEGWEY
                     GIELIAPEMDFWGLDL"
     gene            2810..3694
                     /locus_tag="VFR42_05215"
     CDS             2810..3694
                     /locus_tag="VFR42_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU0368584.1"
                     /translation="MRNLIVNADDLGWTAGVNRGIAEAHRHGIVTSTSLLANGCAFED
                     AVATASEMPGLGVGVHLNLSDGAPIAPAEQVKSLLDAHGKFAGGPESLLMRLTTKSLQ
                     PKEVEKEWEAQIEKVRGAGIRPTHLDGHKHVQMLPGLFGIALRLAKRHGILAVRVSHE
                     ASPLRTALSDGNESSGVALKQGVQARGLKLLARDAREMAERAGIVTADYFCGIAQTGV
                     LTKAGVQKLLGSLPEGTTELMCHPGYADEDLRKSHTRLQESRQTELQILTDKTIRKSV
                     AELGIRLINYEQASVTGR"
     gene            3744..>4238
                     /locus_tag="VFR42_05220"
     CDS             3744..>4238
                     /locus_tag="VFR42_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0368585.1"
                     /translation="MSSEANIKYSIVVPFFNEQENIPPLYMKLTEVMDSIGEPYELVF
                     VDDGSRDSTFRVLSEIFEHDRRVNLIRLRRNFGQTPALKAGFDFARGSIIISMDGDLQ
                     HDPEEIPRFLEKIEEGYDLVSGWRYARRDHWLMRQIPSRTANWMMAKLSGVDLHDFGT
                     TFKAY"
BASE COUNT          941 a         1072 c         1379 g          846 t
ORIGIN      
        1 attcgagtca gggcaccagc ggaaagtcaa ccaacaccag ctgcgtgttc aacgacatta
       61 ccggaacgac ctcaaaagac ggcctgacgc caacgctgaa caattcggtt ccctgcaccg
      121 gcgcttcgac caattgcagc aaaaccagca gcggcggatt tggagttttg acatcggcgg
      181 gcagcccggc gttcacggcg ggattgggct acgacctggc aacgggactg ggcacgccaa
      241 acgtggcgaa tctgatcaac gcgtgggccg ccgtcagcgg gacgggcagt aatgcgacta
      301 ccgtaacgct ggctccggcg accatcaatg gaacagctgg ttccaatttt actttgagtg
      361 ggactgttac gaagtcctcc ggcagcggga ccccgaccgg tgtggtagtt tttgaaaatg
      421 cggcaaccgg aactccagca ggcaacattc cgctgctgaa tctcttgttt ggcggaattt
      481 ccaacgatcc cgctactctc aactccggcg ggagctacag cgtctcgaca ggattgcttc
      541 ccgctggaac ctattctttg aaagcgcatt atgtcggcga tgctacgttt gctccgagcg
      601 attcagctcc catctcagta acgctttcaa agcaagctag caccgtagta gtaagtttcg
      661 taacggcgaa cgggacgatc acgacgaact cgcaaagtgt acagtacggc tcaccttaca
      721 tcctccgagt cgacgtaaac ggaacatctg ggtcttgcac ggatagcaat ggcaatgtgg
      781 ttcgcatctg cccgaccggc acgatcacac tgaaagataa tggcagcgcg ctgaatgatt
      841 tcccgaatgc gcaaaatgcc aacgcgacaa acgtggcgaa gctaaacaac cgcggatttg
      901 cagaagacca acctatccaa ctgaacgtcg gggcccaccc aatcacagct tcttatacgc
      961 cagatccgac cagcagctac ctcgtcccaa ctaccggcag caacacttta tcggtgacga
     1021 tcacgcaggc gacgacctcc gttgtggtga cgccgagcgt gaccagtgtc gtgtcgggtg
     1081 gaagcatcac gctgacggct accgtgaatt ctcagagcaa ttcagcgcaa gggccgaccg
     1141 ggacggtgca attcaagaac ggcagcacga atttgggggc ggcggtgacg tgcacgccga
     1201 aaggcgcgga ttccaacggc tctaccatgg tgggcgcgct ttgcacggcg caattggcga
     1261 ccacgctctc ggcgttgccg ccgggatttt ttgtggagca gcgaccgcgc aacactccgt
     1321 ttgtaatagt cacatcactg gcggcgttgt tggcgatgct gagcttcgta ctagcgctga
     1381 aattgggcgg acgacgccgg cgcttcgctt acgcgggagc gatgtttgcg ctgatcgctg
     1441 cgtccgcgct ggctggatgc aacggaggta gtccgagcgg aggcggtgga ggcggaggat
     1501 cggcacgcac aatctcggcg gcgtacagcg gcgacacgaa ctacgcgact tcgacgggat
     1561 cgacgtcggt tacggtagag tgagaacagc acttcaatgg ttaaaagatt aaagagttta
     1621 attctggaga acggctgacg cgaaatttcg taagtcgctg atttagctgg gcatcggcga
     1681 tggccgtttg gagcgggaac tatagttctg gtttcgcggc gagatgaacc gttttggttc
     1741 actttttgtg agtgttggcc cgtccctaaa ctcctgattc cacgagcata ggcgggcaag
     1801 gtttcgaact gcaaaaaaaa agcagcggat aaagccgctg ctacaagatg ggcgcaattt
     1861 ccccactccg aactctggca ccgcggattg atttggcgag ccaaaatttg ttcgatttag
     1921 ttgatcaccg gggggaagaa aacgttccag aaatttggcg acggcgaggc gaattcaatc
     1981 gctaccaggg agccgtcggg actgaactgc ggcggacggg ccacgtgggc ttccactttt
     2041 ttctgggtgg tggcgttttc cagagtgagc ttggtgccct gctgaagcgc ttcgcttaaa
     2101 acgatgagcg caccggtggc gctgacggtg tgcgtggcgc cttcaagagg ttgttttttg
     2161 ccggcgacgg agatgcgaac cggcaccctt agcagcacgc gttgagcgcg gcggcgttct
     2221 tcggatgtga gcggccgggt aagaccgggt ttcaccggcg taccgagaac gtgtttatgc
     2281 ggaggcgttg gaggtttcat aggtcgagtc cccagaaatc catttccggc gcgatgagtt
     2341 cgatgccgta ctcccagccc tcggggcggg gattgccgcg ccagacgacc acggcggcgt
     2401 tggtggagcc ggtggcgagg ttggtgagcg agaggcgatg ctcgagatcg agcgcatcct
     2461 tgaagatgac catgcagccg tagggattga cgacacgtgt gaaggattcg gcgacaacgc
     2521 gcttgccttc ggcttcccat tcggcgcgaa cgcgcacgcg ggaattcatg cggtggccgc
     2581 gacgtttttc gctgtagcgg ttgccgactt cgcgcagtcc tacgcgcttc tgctcaggcg
     2641 ttttgacctg cattcttacc tcacgtagcc atgctagagt tgggagaaat cacggtcaat
     2701 ggtacggaag cggcgcggat gggaaatgag tagctgtccg aaggtacagg aaatgcggaa
     2761 gttttgagtc gtcagtatta cgttgtgcgg gctctcggtg tgggagaaga tgcggaatct
     2821 gattgtgaat gcggatgatt tggggtggac cgcgggagtg aaccgcggga tcgcggaagc
     2881 gcatcggcat ggaattgtga ccagcacttc cctgctggcg aacggctgcg cgtttgagga
     2941 tgccgtggcc acggcaagcg agatgccggg gttaggagtg ggagtgcatt tgaatttaag
     3001 cgatggagcg ccgatcgctc ccgcggagca agtgaagagt ttgctggatg cgcacggaaa
     3061 gtttgcgggc ggaccggaga gtttgctgat gcggctgacc acgaagagcc tgcagccgaa
     3121 ggaagtggag aaagagtggg aggcgcagat tgagaaagtg cgcggcgctg gaattcggcc
     3181 gacgcacctg gatgggcata agcatgtgca gatgctgccg ggcttgtttg gaattgccct
     3241 gcggctggcg aagcggcatg ggattctggc ggtgcgggtt tcgcacgaag cgtcgccgct
     3301 gcggacggcg ctttctgacg gaaacgagtc gagcggcgtg gcgctgaaac agggagtgca
     3361 ggcgcgggga ctaaaattgt tggcacgcga tgcgcgggaa atggcggagc gcgctggaat
     3421 cgtgacggcg gattattttt gcgggattgc gcagacagga gtgttgacga aagccggagt
     3481 gcagaagttg ctgggcagct tgccggaagg gacgacggaa ctgatgtgcc acccagggta
     3541 tgcagacgag gatctgcgca aatcccacac gcgtttgcag gaatcgagac agacagaact
     3601 gcagatattg acagacaaaa ccatacgaaa aagtgtcgca gagcttggga tacgcctgat
     3661 aaactatgaa caggccagcg taacgggcag atagggcggc tgaagcagcg accagcccga
     3721 aacgcggcct tccgatcgtc aaaatgagca gcgaagccaa catcaaatac tcgatcgtgg
     3781 tgccgttctt caacgagcag gagaacattc ccccgctcta tatgaagctg acggaagtaa
     3841 tggactcgat cggtgagccc tacgaactgg tgtttgtgga tgacggcagc cgggacagca
     3901 cgtttcgggt gttatcggaa atttttgagc acgaccgccg ggtgaacctg atccggctgc
     3961 gcaggaattt cgggcagacg ccagcgttga aagcgggctt cgattttgcg cggggcagca
     4021 tcatcatttc gatggacggc gacctgcagc acgatcccga agagattccg cggttcctgg
     4081 aaaaaattga agaaggttat gacctggtga gcgggtggcg gtatgcgcgg cgcgaccact
     4141 ggctgatgcg gcagattccg agccggacag cgaactggat gatggcgaaa ttgtccggcg
     4201 tggatttgca cgatttcggg acgacgttca aggcgtat
//
LOCUS       DASTPM010000295         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1221423, whole genome shotgun sequence.
ACCESSION   DASTPM010000295 DASTPM010000000
VERSION     DASTPM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221423"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VFR42_05225"
     CDS             complement(<1..1703)
                     /locus_tag="VFR42_05225"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEU0368586.1"
                     /translation="MRRRMILLLLAGALWGQSGDFRTTLFPIFEQANCRACHNTDGVA
                     SATRLHFPEADANVDKIAAFGNSLVVLVDRQNLEASLLLRKPTARMPHTGGERIKQDS
                     PDEAVLKTWIARLAQLSGAELATALKYRELEKEGGGSAQDAQLRRLTHSQYNHTVRDL
                     LGDQTGPAMQFPPEDFVNGFLNQSRGQSLSPLLLEAYSNAAEKLSRSAFRGGDTRGLL
                     SCKPSPECGKRFVREFGLKAFRRPLEPEEQQRYESLLAKEPDFLKGAQLVVEAMLQSP
                     NFLFWINTASDPKLKAWATASRLSYSVWDTMPDAELFAAAKRGELATRAGVEKQVRRM
                     LSDPRAHESLDEFVSQWLRFDRLTTASKDRRKFPYFTRETANAMITEARTFVSDLVWN
                     DGDFMDLFRANYGYISPELARIYGVPAPARDFERVAFPANSERAGILGQGLFLALTAK
                     PEDSSPTARGLFVREQFLCQHVPDPPPGVNTNLPPVTEAKPQTNRDRMSEHTTNTSCA
                     TCHKLMDAVGFGLEKFDAIGARREQMVLEFRKKSYEDEDEDGKHPVPKRIITLPLNTN
                     A"
     gene            1771..>2511
                     /locus_tag="VFR42_05230"
     CDS             1771..>2511
                     /locus_tag="VFR42_05230"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368587.1"
                     /translation="MRQLIAALLFFAVAAPAATEREVAEWVLRWEGSLILEGSNEPIR
                     NLSQLPPSDFHIASIDLTAAVMRPVELRKLEGLTHVRQLYLPGRIWNPGAGNEDKTGV
                     FEALASLTSVERMAFSWHFNSNIEVDDKDIDKLALWSGLKQFRCAQCRLAKPKLSAFS
                     QLRDLDLSDNPFTDEGMASLAGLRNLKHLILRNTLVTDDGLKYLQDLTALEELDLSGT
                     RITGRGLEHLRNLKSIRSLNLLGAQVPDE"
BASE COUNT          509 a          808 c          664 g          530 t
ORIGIN      
        1 tacgcatttg tgttcagcgg caacgtgatg attcgcttcg gtacgggatg ttttccatcc
       61 tcgtcttcgt cctcatagga tttcttgcgg aattcgagga ccatttgttc tctccgtgcc
      121 ccaatcgcat cgaacttctc aagtccgaat cccacggcat ccatcagctt gtggcaggtg
      181 gcgcacgagg tgttggtcgt gtgttccgac atccggtcgc gattggtctg cggcttcgcc
      241 tccgtgaccg gtggcagatt cgtgttcacg cccggcggcg gatccggcac atgctgacag
      301 aggaactgtt cccgtacaaa aaggcctcgc gccgtgggcg atgaatcttc cggtttcgcc
      361 gtcaatgcca gaaacaaacc ttgccctaaa atccccgccc gttcagagtt cgctggaaat
      421 gccacccgct cgaaatccct ggccggtgcc ggtacgccat agatccgggc tagttccggg
      481 ctgatgtagc cgtaattagc cctaaaaaga tccatgaaat cgccatcgtt ccataccaga
      541 tcggagacaa acgtgcgcgc ctctgtgatc atggcgttcg ctgtttcgcg cgtaaaatac
      601 gggaattttc gccggtcctt gctcgcggtc gtcaaccggt caaatcgcaa ccattggctt
      661 acaaactcat ccaacgattc gtgagcccgc gggtcggaca gcatgcgccg cacctgcttc
      721 tccacgcccg cccgcgtcgc cagttcgcca cgcttagcag cggcgaaaag ttcggcatcg
      781 ggcatcgtgt cccacaccga atacgaaagc cggctggccg tcgcccacgc cttcaatttc
      841 gggtctgacg cagtgttgat ccaaaacagg aagttcggcg attgcaacat cgcctccact
      901 accaactgcg cgcccttcaa gaaatccggt tcttttgcca gcagcgattc gtagcgctgc
      961 tgctcctccg gttccagggg gcgccggaac gccttcagac cgaattcccg cacaaaccgc
     1021 ttcccacact ccggcgaagg cttacaggaa agcagcccgc gggtatcgcc accgcgaaaa
     1081 gccgatcgcg acagcttctc cgcggcattg ctataagcct ccaacagcag cggcgaaagg
     1141 ctctgcccgc gggactggtt caagaatccg ttgacgaagt cctccggcgg aaattgcatc
     1201 gcgggtccag tttggtcgcc cagtaaatcg cgcaccgtgt ggttgtattg gctgtgggtc
     1261 aagcgtctca gttgcgcatc ctgcgccgag ccaccgccct ctttttccag ctcccgatat
     1321 ttcagcgccg tggccagttc ggcgcctgac aattgtgcca gcctcgcaat ccacgttttc
     1381 aataccgctt cgtcggggct gtcctgctta atccgctcgc cgccggtgtg cggcatccgc
     1441 gccgtcggct tccgcagtaa cagtgacgcc tccaagtttt gccggtccac caaaacaacc
     1501 agcgaatttc caaaagcggc gatcttatcg acgttggcat cggcctcggg aaaatggagt
     1561 cgagtcgcgg acgccacgcc atcggtgttg tggcaggcac ggcagtttgc ctgttcgaaa
     1621 atcgggaaaa gggtagtccg gaaatcgccg ctctgccccc atagggcgcc cgccaaaagc
     1681 aacaaaatca tccgcctacg cacgtaaaat ctccatcaga ccttgaggct ttcttcagcc
     1741 gcaccgctcg tccctgtaga atagcaggag atgcgccagc tcattgcggc cctcctcttt
     1801 tttgcagtcg cggcgccggc agcgaccgaa cgcgaggtcg ccgagtgggt gctccgctgg
     1861 gaaggaagtt tgatcctgga agggtcaaac gagcccatcc gtaatctctc ccaattgcct
     1921 cccagcgact tccacattgc ctccatcgat ctcaccgccg ccgtcatgcg ccccgttgag
     1981 ctccgcaaac tcgaaggcct gacccatgtg cgccagcttt atctgccggg ccgaatctgg
     2041 aacccgggcg cgggcaacga agacaagacc ggtgtgtttg aagccctggc ctcacttacc
     2101 tccgtcgagc gcatggcctt cagctggcat ttcaactcga atatcgaagt cgatgacaaa
     2161 gacatcgaca agctcgctct ctggtcggga ctaaagcaat tccgctgcgc tcaatgccgc
     2221 ctcgccaaac ccaagttgtc ggccttctcg caattgcgcg acctcgatct cagcgacaat
     2281 cccttcaccg acgaaggcat ggcttccctc gccgggctaa ggaacctgaa acatctgatt
     2341 ctgcggaaca cgctggtcac cgacgacggc ctcaaatatc tgcaggattt gaccgctctg
     2401 gaagaactcg atctgtccgg gactcgcatc acgggccgcg gcctggagca tctcagaaac
     2461 ttgaaatcga ttcgcagctt gaatcttcta ggcgcgcaag tgcccgatga a
//
LOCUS       DASTPM010000296         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_442925, whole genome shotgun sequence.
ACCESSION   DASTPM010000296 DASTPM010000000
VERSION     DASTPM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442925"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="VFR42_05235"
     CDS             complement(<1..1162)
                     /locus_tag="VFR42_05235"
                     /inference="COORDINATES: protein motif:HMM:NF040546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEU0368588.1"
                     /translation="MNTLLLATYELGRQPFGLASPAAWLRKRGHPVRCFDLSRQVLDE
                     QAMREAQLIVFYLPMHTATRLALQWAGIIRAQNPSARLCACGLYAPLNEAHLRAAGFG
                     FVLGPEFEEDLSKLAEDLSTGEMLQVSRKGHSSLPRLTFEMPDRAGLPALQKYAHVVL
                     PHGSHRTAGYTETSRGCKHLCRHCPIVPVYDGQFRVVPREIVLADIRQQVAGGAQHIT
                     FGDPDFFNGVTHAARIVDELHAEFPHLTYDVTIKVEHLLRHADVLPKLARTGCLFVTS
                     AVESFDDEVLLKLDKRHTRADFFQALDVCAAAELALQPTFVPFTPWTTLATYQDLLEV
                     IADRDLISSVPSIQLAIRLLIPSRSRLLELPDISGIVSDFDSERLVYPWKHSD"
     gene            1272..>3759
                     /gene="acnA"
                     /locus_tag="VFR42_05240"
     CDS             1272..>3759
                     /gene="acnA"
                     /locus_tag="VFR42_05240"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU0368589.1"
                     /translation="MAISATNSFGTRDKLNVGAQSFDIYRLEHLERQSSESMARLPFS
                     LRILLENLLRNEDARFVHKEDIQGLANWTPKAAEKEIAYMPARVLLQDFTGVPAVVDL
                     AAMREAVKKMGGNPKRINPLFPAELVIDHSVQVDSFGNANAFGLNAQLEFQRNAERYA
                     FLRWGQTAFQNFKVVPPETGICHQVNLEYLARVVCAVPSGNRFEAYPDSLVGTDSHTT
                     MVNALGVFGWGVGGIEAEAAMLGQPSSMLIPQVVGFKLHGKLPEGATATDLVLTVTEM
                     LRKKGVVGKFVEFYGSGLSTLSVPDRATIANMAPEYGATMGFFPVDAETLAYLQFTAR
                     PHEQIALVEAYCKEQGLFRTDATPDPSFTDTLQLDLGTVEPTVAGPKRPQDRVPLRMA
                     KSSFTKIIEGAPTKQAHVQNNGDSFELSSGAVVIAAITSCTNTSNPSLMLGAGLLAKK
                     AVERGLQVKPWVKTSLAPGSKVVTDYLAKAGLTPYLDKLKFNLVGYGCTTCIGNSGPL
                     PDAIGHAIKENNLVAVAVLSGNRNFEGRIHPLVRANYLASPPLVVAYALAGRMDVDLT
                     NEPLGNDSAGKPVYLHEIWPTPQEVEATVRQAVTQEQYRKQYAAVYDGDEHWKSMAVP
                     EGDLYQWDAHSTYIKLPPYFEKMAKTAPPLADIHGARVLAVLGDSITTDHVSPAGSIG
                     VDTPAGKYLIANGVKPHEFNSYGARRGNHEVMARGTFANIRLKNQLAPGTEGGWTTYQ
                     PGGEQMSIYDASVKYREAGVPLVILAGKEYGSGSSRDWAAKGPKLLGVRSVIAESYER
                     IHRSNLVGMGILPLEFKAGETAAMLGVSGHE"
BASE COUNT          824 a         1117 c         1110 g          708 t
ORIGIN      
        1 gatcggagtg cttccatgga tataccagcc tctccgaatc aaaatcgctg acaattcccg
       61 agatgtccgg aagttccagc aggcgtgagc gcgacggaat cagtaagcga atggccaact
      121 ggatcgaagg cacggaagaa atcaggtcac ggtcggcaat gacttccagt agatcctggt
      181 aggttgcgag cgtcgtccac ggcgtaaacg gaacgaatgt gggctgcagc gccagctccg
      241 cagcggcgca cacatcgaga gcctggaaaa agtctgcgcg cgtgtggcgt ttatctagct
      301 tgaggagcac ctcatcgtcg aacgattcga ccgcgctggt gacgaacaga caaccggtcc
      361 gcgccagctt cgggagcacg tctgcgtgcc gcagcagatg ctcgacttta atagttacgt
      421 cgtaggtgag gtgcgggaac tccgcatgca attcgtccac aatgcgcgcg gcgtgcgtca
      481 cgccgttgaa gaaatccgga tcgccaaagg tgatgtgctg cgcgccgccg gctacctgct
      541 ggcgaatgtc agccaaaaca atctcacgcg gcacaacgcg aaactgtccg tcgtaaaccg
      601 gcacgattgg acaatgccgg cataggtgct tgcaccctcg cgaagtttct gtatagcccg
      661 ccgtacgatg cgaaccatgg ggcaaaacca cgtgggcgta tttttgcagt gccggcaagc
      721 ctgctcggtc cggcatttcg aaggtgagac gcggcaagga tgagtggccc ttcctactga
      781 cctgaagcat ctctccggtg gaaaggtctt ccgcaagctt gctaaggtct tcttcaaatt
      841 ccggcccaag gacaaagccg aatccggccg cgcgcaggtg cgcctcattc agcggggcgt
      901 acaacccgca agcgcaaagc cgcgccgagg gattctgcgc gcgaataatt ccggcccatt
      961 gcagcgccag ccgggtggcc gtatgcatcg gcaagtagaa aacgatgagc tgcgcttcgc
     1021 gcatcgcttg ttcgtcaagc acctgtcgtg aaaggtcgaa gcaccgcacc ggatgcccgc
     1081 gcttgcgcag ccaggccgcc ggcgaagcca acccaaacgg ctggcgaccc aattcatacg
     1141 tagcgagcag caaagtgttc acgacgaacc cattataacg cgctgctcct aacatttcag
     1201 acgccccacg cacgccttgc actttaagtt cggttatcat agaaggttcg taacgctcga
     1261 aaggaccgca gatggctatc tccgcaacga attcgttcgg cacccgcgac aagctgaatg
     1321 tcggcgccca atctttcgac atttaccgcc tcgagcatct cgagcggcaa agctcggaaa
     1381 gtatggcgcg actgcccttc tcgctgcgga tcctgctgga gaatttgttg cgcaacgaag
     1441 atgcgcggtt cgttcacaag gaagacattc aggggctggc gaactggacg cccaaggccg
     1501 cggaaaaaga aatcgcgtac atgcccgcgc gcgtattgct gcaagacttt accggcgtgc
     1561 cggctgtcgt tgatcttgcg gcgatgcggg aagccgtcaa gaaaatgggc ggtaatccga
     1621 agcgcatcaa cccgctcttc cctgccgaat tggtgattga ccactccgtc caagttgaca
     1681 gtttcggaaa cgcgaacgca tttggcttga acgcgcagct tgaatttcag cgcaacgcag
     1741 aacgttatgc gtttctccgc tgggggcaaa ccgcttttca gaatttcaaa gttgttccgc
     1801 cggaaaccgg catctgccat caagtcaacc tggagtatct cgcgcgcgta gtttgcgccg
     1861 ttccgtcggg caatcgcttc gaagcctatc cggattcgct ggtcggtacc gattcgcata
     1921 ccaccatggt gaatgcgctc ggcgtgtttg gctggggcgt tggcggcatc gaagcagagg
     1981 ccgcgatgct gggccagccg agttcgatgc tgattccgca agttgtcggt ttcaagctgc
     2041 acggcaagtt gcccgaaggt gcgacggcca ccgatttggt cctgaccgtg acggagatgc
     2101 tgcgcaagaa aggcgtggtc ggaaaatttg tcgagtttta cggaagcgga ctatctacct
     2161 taagcgttcc cgaccgcgcc accatcgcca atatggctcc cgaatatggc gcgacgatgg
     2221 gcttctttcc ggtcgatgcc gagacgctgg cgtacttgca attcaccgcg cgaccgcacg
     2281 aacaaattgc gctggtggag gcgtactgca aggaacaggg ccttttccgt acggacgcga
     2341 cgcccgaccc gtccttcacg gacacattac aattggacct cggcacggtg gagccgacgg
     2401 tggccggacc gaagcgtccg caggatcgcg ttccgctgcg catggccaaa tcatcattca
     2461 ccaaaatcat tgaaggcgca cccacgaagc aagcgcacgt gcagaacaac ggcgactcgt
     2521 ttgaattgtc cagcggcgcc gtggtgattg ccgctatcac cagttgcacc aacacgtcga
     2581 acccgtcgct gatgctcggc gccggcttgc tcgccaagaa ggccgtggag cgcgggctac
     2641 aagtaaagcc gtgggtgaag accagtctcg cgccgggatc gaaagtggtt accgattacc
     2701 tcgcgaaggc cggcctcacg ccgtatctcg ataagctgaa attcaatctg gtaggctacg
     2761 gctgcacaac ctgtatcggc aacagcggcc cgctgcccga cgccatcggc cacgccatta
     2821 aggaaaataa tctggtagca gtcgcggtct tgagcggcaa ccgaaatttc gaaggccgta
     2881 ttcatccgct ggtccgcgcg aactatcttg cctcgccgcc gctggtggtt gcctacgcgc
     2941 tggcgggacg gatggacgtg gacctgacca acgaaccgct cggcaatgat tccgccggaa
     3001 agccggttta cctgcatgaa atctggccga ccccgcagga agtcgaagca accgtgcgcc
     3061 aggcagtgac ccaggagcag tatcggaaac agtacgccgc ggtttacgac ggcgacgaac
     3121 attggaagag catggccgtg ccggaaggcg acttgtatca atgggacgcg cactccactt
     3181 acatcaagtt gccgccgtac tttgaaaaga tggccaagac cgcgcctccg ctcgctgaca
     3241 ttcacggcgc gcgcgtcctg gctgttctcg gcgacagcat caccacggat cacgtctctc
     3301 ccgccggctc gattggcgtg gacacacccg ccgggaaata tctgatcgcg aacggcgtca
     3361 agccgcacga gttcaattcc tacggtgcgc ggcgtggcaa ccacgaggtg atggcccgcg
     3421 gaactttcgc caacattcgg ctgaaaaacc agcttgcgcc cggcaccgaa ggcggttgga
     3481 ccacctatca gccgggcggc gagcagatga gcatttacga cgcatcggtg aaatatcgcg
     3541 aagctggtgt gccgctggtc attctcgccg gcaaagagta tggctccggc tcatcgcgcg
     3601 attgggccgc caagggaccg aaactcctgg gtgtccgctc ggtcatcgcc gaaagctacg
     3661 aacgcattca ccggagcaac ctcgtcggaa tgggcattct gccgctcgaa ttcaaagcgg
     3721 gtgaaactgc ggcgatgctc ggcgtctccg gtcacgaaa
//
LOCUS       DASTPM010000297         6169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_68011, whole genome shotgun sequence.
ACCESSION   DASTPM010000297 DASTPM010000000
VERSION     DASTPM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68011"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1097)
                     /locus_tag="VFR42_05245"
     CDS             complement(165..1097)
                     /locus_tag="VFR42_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0368590.1"
                     /translation="MREPALGLKTSLLNGGSFLKRIHENLRSVWRLPWAPIPSAGAPI
                     HLLDERRAWTAPAAHVGSVLVHGVLFALILALTLWAPKRREIDPFTVLPGRSIEYWSD
                     HASHANGPDGGGKAGGGGNHNPLPPPAGELAPHSRIQLAPPRLPDGRIHPLPDPATIL
                     DAEAPVVVAPVNELGLPWMKDKTDSAGPGTSGVGNTSGTSMGRNGLDGDGESDSSLAY
                     NRVASQVICRVCPDPLYSEEARKTKLQGSVLLAVLVGADGRVNEVRVLRGLGMGLDEN
                     AVQAVRGWQFLPAKDAAQHPVASWIRVETMFRLF"
     gene            complement(1201..2406)
                     /locus_tag="VFR42_05250"
     CDS             complement(1201..2406)
                     /locus_tag="VFR42_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0368591.1"
                     /translation="MPGTLLNLGPPRNPEKTVSGENSSASTYGVPRFGRPPMLMPDNF
                     WSNLKQFLFERPVKVRGDVKSTLMPAQYGDGFWNNVSEFFTSRPVPKGTSDSRLSTGW
                     VEGFGGFGTRIKEFFSPAKQAPLPFEVKPVRVKEIWTKDENFGWTQVISISLHVVLIG
                     LIVVPLFTHVLPPPTEAMNKTDVLPLDISPYAAKLPSGTKPAGGGGGANDHTTTPVNR
                     GKLPKFKWTQFTPPQVKIMNPAPKLPMDPSLLGPPDLRVSNIQAPTFGDPLANGVSDS
                     LGHGNGTGIGSGSGGGLGPGEGGGTGGGAFRAGVNGVGMPSCIYCPDPQYSDEARKAK
                     YQGVVVLMVVITLDGRATNIQVVKSPGLGLDEKAIEAVRQWKFRPAVGPGGKAVPAQV
                     PIEVTFRLF"
     gene            2639..2818
                     /locus_tag="VFR42_05255"
     CDS             2639..2818
                     /locus_tag="VFR42_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368592.1"
                     /translation="MEDLQKQARQQAMVMAAAIFAARSLTDWDGGRSPRAIAAAADAI
                     AKAKFIVEEIERKLR"
     gene            complement(3486..4124)
                     /locus_tag="VFR42_05260"
     CDS             complement(3486..4124)
                     /locus_tag="VFR42_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0368593.1"
                     /translation="MNCKMSRSTIAAIVVTSLWSIGAPALETNRDERQELTRLAELTR
                     WKEGTVVADIGAGDGTYSFLAAERVGPSGRVYATEIDKEKLQVLNAEAAKRKLKNIVV
                     VEGSTDDTKLPSSCCDTIFLRHVYHHLTQPQDFDRNLVRSLRPGAYLAIIDFPPSPNL
                     PPVEGVPKNRGGHGIPEKLVVEELATAGLEVEKIVDDWSAQDYCVVFVKRSP"
     gene            complement(4331..5038)
                     /gene="mtgA"
                     /locus_tag="VFR42_05265"
     CDS             complement(4331..5038)
                     /gene="mtgA"
                     /locus_tag="VFR42_05265"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEU0368594.1"
                     /translation="MTATPTTQTKTPLRTGFLRSFIRWLVIGVALLWSLAALILVAVR
                     WIDPPTTAVHIQRRLQAWIHHKPYRERYKFIPLSQISADLQHAVVAAEDAHFYQHHGF
                     DWHEIQIAAEDDLEGDRTRGASTITQQLVKNLFFGTGRSILRKGAEVTLVPVAEFVLG
                     KQRILELYLNVVEWGPGIYGAESACRYYDESAARSIGRQQAARLAAILPAPLKRRPER
                     MNNYSAIILKRMGQMGW"
     gene            5320..>6169
                     /locus_tag="VFR42_05270"
     CDS             5320..>6169
                     /locus_tag="VFR42_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU0368595.1"
                     /translation="MSEPLVLKSTLNLPKTDFPMKAGLPQNEPKQLDAWQEADLYQRI
                     LDARQGKPPFVLHDGPPYPTGTIHLGTGLNKILKDLIVKTKSMAGHYAPYVPGWDCHG
                     LPIETQVEKELGGKGKVPPAEFRKMCREFAARYVEQHKKDFKRLGVFGRWNDPYLTMS
                     FDYEATIAGAFLEFMEKGYVYRGRKPVYWCLYDSTALAEAEVEYEDHISPSIWVKFAV
                     VGPAEGPQANAGGKNEAAKIGSDVSAAIWTTTPWTIPHNRALAFHPDFEYAVVQTEKG
                     KLLLAAD"
BASE COUNT         1324 a         1911 c         1652 g         1282 t
ORIGIN      
        1 tcgtctttgt ccttggccgc ggtgcgaact cgtggcaacc tcatcctgct catcgcggaa
       61 cgaaacggtg cctggcgccg ctacaattgc gcaaatgacc acgacgacaa acaggctgac
      121 acgtttcttg ggcatcggct cggttcttaa ttaaccgcga cagcctagaa caaccggaac
      181 atggtttcga ctctgatcca cgacgcaaca ggatgctgcg ccgcgtcttt ggcaggaaga
      241 aactgccagc cacgcacggc ttgaacagca ttttcgtcca gccccatgcc gagcccccga
      301 agcacccgaa cctcattgac acgcccgtcc gcaccgacca gcaccgcgag cagtaccgat
      361 ccctgcagct tggtcttgcg cgcttcctcg gaatagagcg gatcaggaca gacgcgacaa
      421 atcacctgag aggcgacgcg gttgtaagcc aagctgctat ccgattcgcc gtcgccatct
      481 aggccgttgc gccccatcga ggttccggac gtatttccca ctccactggt cccaggacca
      541 gcggaatcgg ttttgtcctt catccacggt agccccagtt cgttcaccgg agccacgaca
      601 actggcgcct ccgcatcaag tatcgtcgca gggtcgggaa gcggatgaat gcggccatcc
      661 ggtaagcggg gcggcgccaa ttgaattctc gaatgcggcg cgagttctcc tgccggcggc
      721 ggaagtggat tgtggttgcc cccgccaccc gcctttcctc caccgtccgg tccatttgca
      781 tgcgatgcat ggtcggacca atactcgatg cttcggccgg gcaagaccgt aaagggatca
      841 atttcgcgcc gttttggcgc ccatagtgtc agcgcaagga tcaacgcaaa cagcactccg
      901 tgcaccagaa cggaaccgac atgtgccgcc ggagcggtcc acgcacgccg ctcatccagc
      961 aaatggatcg gcgcaccagc cgacggaatt ggcgcccacg gcagcctcca tacgctccgc
     1021 agattctcat gaattcgctt caggaaggaa ccaccgttca acagcgaagt cttcaagccc
     1081 agggctggct cgcgcatcgc agacctctgc agccctccct ctcgcaccac aaacaaaagc
     1141 ggctcccgac gcactgtggg agccgcaaaa acttcaaaag gttgcttagt cgccaattaa
     1201 ttagaagagc cggaaggtaa cctcgatcgg cacctgcgcc ggaacagctt taccgccggg
     1261 cccaaccgcc ggccggaact tccattgccg cacagcctca atcgcttttt catccaatcc
     1321 caagccgggg ctcttgacca cctgaatgtt ggtagcgcga ccatccagcg tgatgaccac
     1381 catcagcacc accacgcctt gatatttggc cttgcgcgcc tcatctgaat attgcgggtc
     1441 cgggcaatag atgcagctcg gcatgccgac tccgttcact ccggcgcgga acgctccgcc
     1501 accggtgccg ccgccttcgc cgggaccaag cccgccgcca gaaccgctgc caattccagt
     1561 gccattgccg tggcccagtg aatcgctcac gccattcgcc agcggatcgc caaaggtcgg
     1621 cgcctgaata ttcgatacgc gcagatccgg cgggccaagc aaagacggat ccatcggcag
     1681 cttaggcgcg ggattcataa tcttgacttg cggcggcgta aactgcgtcc acttaaactt
     1741 gggcagcttg ccgcgattca ccggagtggt tgtgtggtcg ttcgctccac cgccgccacc
     1801 cgctggcttg gtgcccgagg gcagcttcgc cgcgtacggc gaaatatcca gcggcaaaac
     1861 gtccgtcttg ttcatcgcct ccgtcggagg cggaagcacg tgcgtaaaca acggcaccac
     1921 gatcagtccg attagcacca cgtgcaggct gatcgaaatc acttgcgtcc agccgaaatt
     1981 ttcgtccttg gtccagattt ccttcacgcg gaccggcttc acttcaaacg gcagcggtgc
     2041 ctgcttggcg ggcgagaaaa attctttgat gcgcgtaccg aatccgccaa agccttccac
     2101 ccatcccgtc gaaagcctgc tgtcggaagt tcccttgggc acgggccggc tggtaaaaaa
     2161 ttcgcttacg ttgttccaaa acccgtcgcc atactgcgcg ggcatcagcg tggatttcac
     2221 gtcgccgcgc accttcacgg gccgctcgaa caaaaattgc ttcagattgg accagaaatt
     2281 gtccggcatc agcatcggcg gccggccaaa acgcggtact ccgtatgtcg aggcggagga
     2341 gttctccccg gaaacggtct tttccgggtt acgcggcggc cccaagttga gaagtgttcc
     2401 tggcaagtgt ctccgcctat cagtgtttag acgcagcccg acccttctgg gtgcaccttc
     2461 gggtacgatt cgcggcgcct gagaaaggtt gctaccgtca attccgctcc atacccgctt
     2521 ctagcattgt aacaaccgct tgcccaagaa tctacgccca taagccaaag ccgcaacgtg
     2581 ctagaatcca cctcccgctt cgtctgcgac acctcccgtc ctccccggag aaagttctat
     2641 ggaagatctg caaaaacagg cccgccaaca ggctatggtc atggccgcgg ccatattcgc
     2701 ggcgcgcagt ttgacggatt gggacggcgg acgctcgccc cgcgccattg ccgccgcggc
     2761 cgacgccatc gccaaagcca agtttatcgt cgaagagatc gaacgaaagt tgcgatgaac
     2821 ctaggcgctt actctctccg gccgccgacg ctatggctcg cgcggccgga gagcctcgca
     2881 ccctgtctct gcggcttccc cgaaatctcc cgcagcactg ctcaggttta ctgccgcccg
     2941 ggaccaagct cctcgactct cgcttggttc ttcagatagt ctggcactgc gagtgtgtga
     3001 agaagagttg gtcgtagcaa cgctcgtccg ctcggcatcc ccaaccgaag tagtgcgcta
     3061 tggggtctcc aaccgagata tgctcgcgcc gcaatctgaa ccggaaagaa cggcgtgttc
     3121 gacagcacca ggtgcatcgc ccacataaag gcagaatgcg cccatgcttg gtgcatcatg
     3181 gttattgacc ctcgcagaaa tgtgcccaaa atcggagcca aaatcacacc ttccgtccca
     3241 atgatttggt gcgccataaa aacgagtgcg gacctgaccc aataacactg cttctttgtc
     3301 tgcgagactc tggccatagg accttgagcg cggacacaaa tcctgagttg cgagtctaag
     3361 ccgtcacaaa atagacgcta agtggcctaa cgcccacctc agtgcgggca tggcattgaa
     3421 tctgcgttgt tttagtggag gaccaggtca tacctaagca tgcgtcgaac attgcttcca
     3481 tccgctcacg gagagcgctt cacgaaaaca acgcaatagt cctgcgccga ccaatcgtca
     3541 acaatcttct cgacttccaa tccggcggtc gccagctctt ctaccaccag cttctctggt
     3601 attccgtgcc ccccacgatt cttggggact ccttctaccg gtggcaaatt ggggctgggt
     3661 ggaaaatcga tgattgcaag ataagctccc ggcctaagcg aacgcaccaa atttctgtca
     3721 aaatcctgcg gttgcgtaag gtggtgatat acgtgccgta gaaaaatcgt gtcgcagcaa
     3781 ctcgatggga gcttggtgtc atctgtcgac ccctccacta cgacgatgtt tttcagcttg
     3841 cgttttgcag cttccgcatt cagaacttgc aatttttcct tatcaatttc ggttgcataa
     3901 acacgtcccg acggcccaac ccgttcggca gccaggaagc tgtacgttcc atcgcctgca
     3961 ccgatatcgg ccacaacagt cccctccttc catcgagtca actccgctag ccgagtgagc
     4021 tcttgccgtt cgtcgcggtt tgtttctaga gctggggctc ctatggacca gagcgacgtg
     4081 acgacaatgg cggcgattgt ggagcgagac attttgcaat tcattgattg ttcccccctt
     4141 cgagaggggg cttcctagac cgctcgattc ttgcactttt tagaggttac ttggtaccga
     4201 acctctacct attatcccag gtcgcagatc cagttcggat tagggtaatg catcgggagc
     4261 attgcgcatc ccgtggagac cggccttctc actcggtaaa ccaccctaat tgataaaatg
     4321 ttagccgcga ttaccaaccc atttggccca tccgcttgag tataatcgcg ctgtagttat
     4381 tcatgcgctc gggtcgtcgt ttcaggggag ctggcagaat tgccgcgagc cgtgccgctt
     4441 gttgccggcc gatactccgg gctgcgcttt cgtcgtagta acgacacgcc gactctgcac
     4501 catagatacc agggccccac tcgaccacgt tgaggtatag ctccaggatg cgctgtttgc
     4561 cgaggacgaa ttcggccacc ggtaccagtg tgacctctgc gcccttacgg aggattgagc
     4621 ggcccgttcc gaagaacagg ttttttacaa gttgctgcgt aatggtggac gctccgcgag
     4681 tgcgatctcc ttccaaatcg tcttcggcag cgatttggat ctcgtgccaa tcgaatccat
     4741 ggtgctggta gaagtgcgcg tcttctgcgg cgactacagc atgctggaga tcagccgaga
     4801 tttgactgag cggaatgaat ttgtagcgtt cgcgatacgg cttgtggtga atccaggcct
     4861 gcaatcgtcg ctggatgtgc accgcggtgg tcggtgggtc gatccaccgg acggccacca
     4921 gaatcagggc ggccagcgac caaagaagcg ccacgccaat gacaagccat cgaataaagg
     4981 atcggaggaa gcccgtgcga agcggggtct tggtctgtgt ggtcggcgtt gcagtcatat
     5041 ttcaggataa tagcctgaac tggtgccgct gatgatcttg tgcgcgcgag gaaagaccgc
     5101 tgcgttcgac tcaggctact tggacaaagt cccttggtcc tgattcactt tgaactacat
     5161 tacaaaggat aaagtggaat cggaaactgc cccactcccc gtcggggaat ttgtcccttc
     5221 gccgttccgt tgtaaaatct ttggttacac ttccccatgt tttcccgcca ggcttccggc
     5281 gaaaaaccag ggttgagaag ggattgcgac gcggtatcta tgtctgaacc acttgtactc
     5341 aaatccacgt tgaacctgcc caagaccgac tttccgatga aggcgggtct gccgcagaac
     5401 gagcccaagc agctcgacgc gtggcaggaa gccgatcttt accagcggat tctcgatgcc
     5461 cgccagggta agccgccctt cgtcttgcat gacgggccgc cgtatcccac cggcactatt
     5521 catctcggca ccggactcaa caaaatcctg aaagatttga tcgtcaagac caagagcatg
     5581 gccggccact acgcccccta cgttccgggc tgggattgcc acggcctccc gatcgaaacc
     5641 caggtcgaaa aagaactcgg cggcaaagga aaagtcccgc ctgccgagtt ccgcaagatg
     5701 tgccgcgaat ttgccgctcg ctacgtcgag cagcacaaga aagatttcaa gcgactcggc
     5761 gttttcggcc gctggaatga cccttacctg accatgagct tcgattacga agcaaccatc
     5821 gccggtgctt ttctcgagtt catggaaaag ggttacgtct atcgcggccg taagccggtg
     5881 tattggtgcc tctacgattc aacggcgctg gcggaagcgg aagtcgaata cgaagatcac
     5941 atcagcccgt cgatctgggt gaaattcgcc gtagtcggcc cggcggaggg gccgcaagca
     6001 aatgccggcg gcaagaacga agcggcaaaa atcggcagtg acgtttccgc cgccatctgg
     6061 accaccacgc cctggaccat tccccacaac cgcgcactcg cctttcatcc cgactttgaa
     6121 tacgcggtcg tacaaaccga aaaagggaag ttgctgctgg ccgccgatc
//
LOCUS       DASTPM010000298         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1365654, whole genome shotgun sequence.
ACCESSION   DASTPM010000298 DASTPM010000000
VERSION     DASTPM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365654"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VFR42_05275"
     CDS             complement(<1..200)
                     /locus_tag="VFR42_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368596.1"
                     /translation="MAQFKDLARVPNDQVRNFRSDMYLTSEALRIMQKSGKPQLAAAD
                     VAVLKNYKTHIDRATRFIPLWV"
     gene            313..1701
                     /locus_tag="VFR42_05280"
     CDS             313..1701
                     /locus_tag="VFR42_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HEU0368597.1"
                     /translation="MTNTRYDAIVIGAGQAGVPLSQALAGAGMRTALVEREHVGGTCI
                     NEGCTPTKTMVASGRVAYLARRGADYGVQTGALNIELARVRERKRAIVDSFRNGSQGR
                     IEKTKNLELIFGEAEFTGARTVRVRKADEAERTLSAERIFINAGCRPAVPNLEGLSGV
                     PYLNSTSIMELDQVPEHLIVLGGGYIGLEFGQMFRRFRARVTIVQSGAQLLGQEDADV
                     AKAVLEILQEDGIEILLNSRAKSVSGSPGQLQLTAESAGKSREIAGSHLLVAAGRVPN
                     TDSLHLDAAGIATDARRFIRTNDKLETNVAGVYGLGDIKGGPAFTHISYDDFRIIRTN
                     LIEKGSATIADRLVPYTVFIDPQLGRVGISEVEARKSGRAIRVAKMPMNYVARALEMD
                     ESRGFMKAIVDAKSGQILGAAVLGIEGGEIMSQLQIAIMGKLPYTVLKDGVFAHPTLA
                     ESLNNLFGHFQD"
     gene            complement(1787..>2066)
                     /locus_tag="VFR42_05285"
     CDS             complement(1787..>2066)
                     /locus_tag="VFR42_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368598.1"
                     /translation="YGTNELFGNQYYLFRVGYLHDLFTLPPFLGKKIYAIGSYEAGKM
                     YGFPQETAFPNDVAAGVLAETAFGPLFIGGSYGDSGHRKLYFQLGRVF"
BASE COUNT          471 a          468 c          732 g          395 t
ORIGIN      
        1 tttacccaca gaggaatgaa gcgcgtggcg cggtcgatgt gggttttgta attcttgagg
       61 acggcgacat cggcggcggc gagttgcggc ttcccgcttt tttgcataat gcgaagcgct
      121 tcgctggtca gatacatgtc gctgcggaag tttctgacct ggtcgttggg aactctggcg
      181 aggtccttga actgggccat ttcggtgccg acacggaaca gggaattctt gacaagcgaa
      241 gagagggcgg cgcaacgtag tgcgcaggcc cgcgcaaata atttgtgttc ccccaaaaag
      301 tgaggatttt cgatgaccaa tacgcgatac gacgcgattg tgattggcgc ggggcaggcc
      361 ggagtgccgc tgtcacaagc actggcggga gccggaatgc ggactgcgct ggtggagcgg
      421 gagcacgtgg gcgggacgtg catcaacgag ggatgcacgc cgacgaaaac gatggtggcg
      481 agcgggcgcg tggcgtacct agcgaggcga ggagcggact atggggttca aacgggtgcg
      541 ctgaacattg aactggcaag agtgcgcgaa cgcaagcggg cgattgtaga tagcttccgg
      601 aatgggagcc aggggcgaat cgagaagacg aaaaacctgg agctgatctt tggggaggcg
      661 gagtttacgg gcgcgcggac cgtcagagta cggaaagcgg acgaggcgga aaggacgttg
      721 agcgcggaaa ggattttcat caatgcaggg tgccggccgg cggtgccaaa cctggaaggg
      781 ctaagtggcg ttccgtacct gaacagcacg agcatcatgg aactggatca ggtgccggaa
      841 catttgatcg tgttgggagg cggttacatt gggctggaat ttgggcagat gttccggcga
      901 tttcgagcgc gagtgacgat tgttcagtcc ggggcgcaac tgttggggca ggaagatgcc
      961 gacgtggcga aggcggtgct ggaaattttg caagaagatg gaattgaaat actgctgaat
     1021 tcgagagcca agagcgtaag tggaagcccc ggccaattgc agttgaccgc ggagagtgct
     1081 gggaagtcgc gcgagatagc aggcagccat ctgctggtgg cggcgggacg ggtgccgaat
     1141 acggacagcc tgcacttgga cgcagcgggg attgcgaccg atgcgcgccg gttcattcga
     1201 accaacgaca agctagaaac gaatgtcgca ggcgtgtatg gactgggaga catcaagggc
     1261 gggccggcgt ttacccacat ttcgtacgac gatttccgga tcattcgaac gaacctgatc
     1321 gaaaagggaa gcgcgacgat tgcggaccgg ctggttccct acaccgtgtt cattgacccg
     1381 caactggggc gagtgggaat aagcgaggtg gaggcgcgca aatccgggcg cgcgattcgt
     1441 gtggcgaaga tgccgatgaa ctacgtggcg cgggcgctgg agatggatga atcgcgcggt
     1501 ttcatgaaag cgattgtgga cgcgaagagc ggacagatcc tgggcgcggc cgttttggga
     1561 attgaaggtg gggagatcat gtcgcagctg cagattgcga taatggggaa gctgccgtac
     1621 acggtattga aggatggagt gttcgcgcat ccgacgctgg ccgagtcgct gaacaatttg
     1681 ttcgggcatt ttcaggacta ggagccttcc tttagaggct aaagcgcaac tttcgcagag
     1741 ccgcgcacgt cggcctaaac cccaacctgc taacgaatat tggagatcaa aagactcggc
     1801 caagctggaa atatagtttg cggtggccgc tatcgccgta gctgccgccg ataaagagcg
     1861 ggccgaaggc ggtttcggcg aggacgccgg cggctacatc gtttggaaaa gcggtttcct
     1921 gcggaaaacc gtacattttg cctgcttcgt agctgccgat ggcgtagatc tttttgccga
     1981 gaaacggcgg cagggtgaag aggtcgtgca gatagccaac gcggaagagg taatactgat
     2041 tgccgaaaag ttcgttggtg ccgtaa
//
LOCUS       DASTPM010000299         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_529684, whole genome shotgun sequence.
ACCESSION   DASTPM010000299 DASTPM010000000
VERSION     DASTPM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529684"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /gene="terL"
                     /locus_tag="VFR42_05290"
     CDS             complement(<1..1667)
                     /gene="terL"
                     /locus_tag="VFR42_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEU0368599.1"
                     /translation="MTYQVIARAKLPLAEERAELEGSLLKFVEAAWPSLDPAPYQRCW
                     AIDALCEHLQAVTEGQIKKLLVNFPPRSAKTNISSICYPAWTWARSEKSYLSGPQVRF
                     LCGSYNDDLALQNSTKRRRLLQSPFYQRRWGKRFTITLDQNTKTQFDTSAGGSRISTS
                     ARGSLLGIGGDIVILDDPHNTKQAESESERLSALTWWREISTTRLNNPKASALVVIMQ
                     RLHEEDVSGVILSSDHSADWVHLMIPMEYEHRRHCTTVLGWQDPRGLDEEGEPLLEHG
                     VPRDARAAQILEEREGALMWPERFGPKEIVRIKNELGPYMASGRLQQSPQPKGGGIFK
                     REWVELWDPPDRMFPVMSFIVGSVDGAFTEKQTNDPSGMTVWGVFVHPELRKTRVMLI
                     DAWRKHLQMHGAPTPRLGDEVVNIGDTPQIVRRKALLWEKRVGQEWGLVEWVAQTCRK
                     WGVQKLLIENKGPGITAAQELQRLHGREGWSVWLVDPKRSDKVARALAIQPMFSQGLV
                     YAPDRAWVDMWLTELCLFPNAKHDEMVDTTTQALKYLRGVGAVWFDD"
     gene            complement(1702..2196)
                     /locus_tag="VFR42_05295"
     CDS             complement(1702..2196)
                     /locus_tag="VFR42_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368600.1"
                     /translation="MPILKNPRRERFAQLLASGKTAKDAYEIAGYKPSESNGAWLARK
                     EEISSRVAEINNETLERERATAATAAERAAVTRQSLIEKAEAIRAAAMEAGQFSAAVA
                     ATKEIGVLSGIRIERSERGQPGEFDWVEKLSVEELQALAEGKLDIASFKDERASGSGH
                     SRLN"
     gene            complement(2197..>2422)
                     /locus_tag="VFR42_05300"
     CDS             complement(2197..>2422)
                     /locus_tag="VFR42_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368601.1"
                     /translation="GTRCTQRRVFFVMGFGMKDRTDEIDSAILAFRCPKDLVGAIEHQ
                     AAVEGISKRDVARRACIRDLRRAKGQEAEG"
BASE COUNT          475 a          776 c          646 g          525 t
ORIGIN      
        1 tcgtcatcaa accaaaccgc gccgacacca cgcagatatt tcaatgcttg cgtagttgtg
       61 tcgaccattt cgtcatgctt ggcgtttgga aacaaacaaa gctccgtgag ccacatatca
      121 acccatgccc gatcgggggc gtacacgagg ccttgagaaa acatcggctg tatagcgagg
      181 gcgcgcgcga ccttgtcact gcgcttcgga tcgaccaacc agaccgacca accctctctt
      241 ccatgaagtc gctggagttc ctgtgccgcg gtgatgccgg ggcctttatt ctcaatgagc
      301 agtttttgca ccccccattt gcggcaggtt tgggccaccc attcgacaag accccattcc
      361 tgcccgacgc gcttctccca aagcagcgct ttgcggcgca cgatctgcgg cgtgtctcca
      421 atgttaacaa cctcgtcccc gagccgcggc gtcggcgcgc cgtgcatctg cagatgcttg
      481 cgccaagcgt caattaacat aacgcgcgtc ttgcgaagct cgggatgcac gaacaccccc
      541 cagaccgtca tgccgctcgg atcgttggtt tgcttctcgg tgaaagcacc atcgaccgaa
      601 ccaacgatga agctcattac aggaaacata cgatcgggcg ggtcccacag ctcgacccac
      661 tccctcttga aaataccccc gccctttggc tgcggcgact gctgcaaacg accgctcgcc
      721 atgtaagggc ccagctcgtt cttgatccgc acaatttcct ttgggccaaa ccgctcaggc
      781 cacatcaacg ctccctcacg ctcctcgagg atctgtgccg cgcgcgcatc acgcgggacg
      841 ccgtgctcaa ggagcggttc accttcttca tcaagaccgc gagggtcttg ccagccgagt
      901 actgttgtgc agtggcgccg gtgctcatac tccatcggta tcatgagatg gacccagtca
      961 gccgaatgat cggacgacaa gatcacgccg ctcacatcct cctcgtgcag cctttgcatt
     1021 atcaccacga gcgcggatgc tttcgggttg ttcaagcgcg tcgtactaat ttcacgccac
     1081 cacgtcaggg ctgagagccg ctcagactct gactcggcct gcttcgtgtt atgagggtcg
     1141 tccaaaatga cgatgtcgcc gccgatgccg agcaaggagc cgcgagcaga agtcgatatg
     1201 cgagagccgc ccgcactcgt gtcgaattgg gtcttcgtgt tctggtccag cgtgatcgtg
     1261 aaccgcttgc cccaccgccg ttgatagaac ggcgactgca acagcctgcg acgcttcgtg
     1321 ctgttctgca gcgccaggtc gtcgttgtag gagccgcaaa ggaaacgaac ctgtggacca
     1381 ctgagataac ttttctcaga gcgtgcccaa gtccacgcgg gatagcagat agacgatatg
     1441 tttgttttag ctgacctcgg gggaaaattc acgagcagct tcttgatctg gccttccgtg
     1501 acggcttgaa gatgttcgca cagcgcatcg atggcccagc acctctgata aggagcggga
     1561 tcgagcgatg gccacgcggc ctcgacaaac tttagcaggc tgccctcaag ctcggcgcgc
     1621 tcctcggcca gcggcagctt cgcccgcgca ataacctgat aagtcaagcg ctcccgaaga
     1681 gatacgtcca tgtcaggtca atcaattcag ccgagaatgc cctgaaccgg atgcgcgctc
     1741 gtccttgaag ctcgcaatgt ctagcttccc ttcggcaagc gcctgcagct cttcgacact
     1801 cagcttctcc acccagtcga actcgccggg ctgaccccgc tctgaacgtt caatgcgaat
     1861 gccggacagc acgcctattt ccttggtggc ggcgacagcg gccgaaaact gtccggcctc
     1921 cattgccgca gcgcggattg cttcggcttt ttcgatcaac gactgcctgg tgacggcggc
     1981 acgctcagca gcggtggccg ctgtggcccg ctcccgttcg agggtctcgt tattaatttc
     2041 tgctaccctg ctgctaatct cttcttttcg cgcaagccaa gcgccgttac tttcactggg
     2101 tttgtagccc gctatctcat aggcgtcctt ggccgtcttg ccgctggcca gcaattgggc
     2161 gaagcgttca cggcgcgggt ttttcaaaat aggcatttag ccctcggctt cctgcccctt
     2221 ggcgcggcgt aggtctctga tgcacgcccg ccgagccacg tctctttttg agatgccctc
     2281 gacggcggct tgatgctcaa tggcgccgac taggtctttc gggcacctga aggcgagaat
     2341 cgctgaatca atttcgtccg ttcggtcttt catcccaaac cccataacga aaaagacccg
     2401 ccgctgggtg caacgagtcc ct
//
LOCUS       DASTPM010000300         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_270212, whole genome shotgun sequence.
ACCESSION   DASTPM010000300 DASTPM010000000
VERSION     DASTPM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270212"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="hslU"
                     /locus_tag="VFR42_05305"
     CDS             <1..1120
                     /gene="hslU"
                     /locus_tag="VFR42_05305"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU0368602.1"
                     /translation="VKVEASKYTEVGYVGRDVESMVRDLVEMSIDMVREEKLDEVAER
                     AEQAAEERVLDLLLPPPPPAGPGTTESDAASQREQNQRTREKLRAQLREGKLDQRMVD
                     LEVRERMMPSFEVISNQGVEEMDVNFKEMLSGMFGQQKKKRKMSVADAFEYLIQEEEG
                     KLVDMDQVTRIAVERAEQMGIIFIDEIDKVAGRESGHGPDVSREGVQRDILPIIEGTT
                     VNTRYGMIRTDHILFVAAGAFHVTKPSDLIPELQGRLPIRVELQSLKEEDFVRILTEP
                     KNALIKQYIALLETEGLKLTFTDDAVETVAKFATQVNEQTENIGARRLHTILEKVLDE
                     LSFEAPDLKKKTVKVDAAYVNKQLADIVKNQDLSRYIL"
     gene            1137..>2120
                     /locus_tag="VFR42_05310"
     CDS             1137..>2120
                     /locus_tag="VFR42_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368603.1"
                     /translation="MKNRKGPRLFDQSFVALLAFFCFVLVCAMAGCGAPGEPQPPSPP
                     IPAAITDLTAHQQGNGVQLVFTLPGRTTAGERLAEAPATEIFRGTLKPDGKPENKSFR
                     LVYTIPGALADTYAAQGKIRFTDPIAAEELRARPSTLLAYRVRTRASKKKDSADSNTV
                     VAKLYAVADKISAIDVNVTQNAIELSWQAPKIDAGSGETVAAYHIYRGELDAGAPIPT
                     DGDLSQARWKNPPLLLAPAQNNSYSDTFFEFGKTYVYLVRSVTIAEGNPIESDDSAPA
                     IVAPRDTFSPAPPQNVVAAVLPGENDSRIVDLSWSINVENDLAGYRVYRSEK"
BASE COUNT          489 a          576 c          659 g          396 t
ORIGIN      
        1 cgtgaaagtg gaggcttcga aatacaccga agtcggctat gttgggcgcg acgtggagtc
       61 catggtgcgc gacctggtgg aaatgtccat cgacatggtg cgcgaagaga aactggatga
      121 agtcgccgag cgggccgagc aggccgccga agagcgggtg ctggatttgt tgctgccgcc
      181 gccccctccc gccgggccgg gaacgacgga gagtgatgct gcgtcacaac gcgaacagaa
      241 tcagcgcacg cgcgaaaaac tgcgcgcgca gcttcgtgaa ggcaaacttg accagcgcat
      301 ggtggacttg gaagtccgcg agcgcatgat gccctcgttc gaagtcatct ccaaccaggg
      361 cgtggaggaa atggacgtca acttcaagga aatgctcagc ggcatgttcg gccagcaaaa
      421 gaagaagcgc aaaatgagcg tggccgacgc cttcgagtat ttgatccagg aagaagaagg
      481 caagctggtg gacatggatc aggtgacgcg catcgccgtt gaacgcgccg aacagatggg
      541 gattattttc atcgacgaaa tcgacaaggt ggcggggcgc gagtccggcc acgggcccga
      601 cgtaagccgc gaaggcgtgc agcgcgacat tttgccgatc atcgaaggca cgacggtgaa
      661 cacacgctac ggcatgatcc gcaccgacca cattttgttt gtggcggcgg gtgcgttcca
      721 tgtgaccaag ccttccgatc tgataccgga gctgcaaggc cggctgccga ttcgcgttga
      781 gctgcaatcg ctaaaagaag aggactttgt gcgcatcttg acggagccga agaatgcgct
      841 catcaagcag tacatcgccc tgctcgaaac ggaaggtttg aagctgactt tcaccgacga
      901 cgctgtggag accgtagcga agttcgccac ccaggtgaac gagcagacgg aaaacattgg
      961 cgcgcggcgg ctccatacga ttctggaaaa agtgctcgac gaactgtcgt ttgaggcgcc
     1021 ggacctgaag aaaaagacgg tgaaagtgga cgccgcgtac gtgaacaagc agctggcgga
     1081 catcgtgaag aaccaggatt tgagccgcta cattttgtaa tgactgacaa ttgaaaatga
     1141 aaaacagaaa agggccgcgc ctttttgacc agtctttcgt ggcgctcctg gcttttttct
     1201 gttttgtgct ggtttgcgcc atggcgggtt gtggcgctcc cggcgaaccg cagccgcctt
     1261 cgccgccgat tcccgcggca attacagatt tgaccgccca tcagcaaggc aacggtgttc
     1321 agctggtttt cacattgcca gggcgcacga ccgccggcga acggctagcc gaagcgcccg
     1381 cgacggaaat ctttcgcggc acgctgaagc ccgacggaaa gccggagaac aaatcgttcc
     1441 gtttggtgta cacgattccc ggcgcgctgg ccgacaccta cgccgcgcag ggaaagattc
     1501 ggtttaccga tccgattgcg gcggaagagc tgcgggcgcg gccgagcacg ctgctggcct
     1561 atcgggtgcg aacgcgcgcg tcgaaaaaga aggattccgc cgactcgaac acggtggtcg
     1621 caaagttata cgccgttgcc gacaaaatct ctgcaataga cgtgaacgtt acgcagaacg
     1681 caatcgagct ttcctggcag gcgccaaaaa ttgacgcagg ctccggcgaa acagttgccg
     1741 cctatcacat ttatcgcggg gagttggatg cgggggcacc tatcccaaca gacggcgatc
     1801 tgtcgcaagc taggtggaaa aatccgccgc tcttgctcgc gcccgcgcag aacaattctt
     1861 atagcgatac gtttttcgag tttggcaaga cgtacgtcta cctggtgcgc agtgtgacca
     1921 ttgcggaggg aaacccgatt gaatcagacg actcggctcc ggcaatcgtg gcgccgcgcg
     1981 ataccttctc tccggctccg ccgcaaaacg tcgtggcggc ggttcttccc ggcgagaacg
     2041 actcgcgtat cgtggatttg tcgtggtcca tcaatgtaga gaacgatctg gcgggctatc
     2101 gagtctatcg ttctgaaaag
//
LOCUS       DASTPM010000301         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1221569, whole genome shotgun sequence.
ACCESSION   DASTPM010000301 DASTPM010000000
VERSION     DASTPM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221569"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1231
                     /locus_tag="VFR42_05315"
     CDS             341..1231
                     /locus_tag="VFR42_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEU0368604.1"
                     /translation="MNERGLRSGWRIVIYLFQILVFSLAVNFLLGSVLHLPKTAQPPM
                     WQMTLQEGLSFLLVLLPAVVMSRIESRSPGDYGLPLNELLGARFWHGCVLGLVEISVL
                     IACIAAFGGYKFGALDLHGSAILRWALLWAFFFLLVGLFEEFAFRGYLQFTLADGIGF
                     WLAAWILSLGFGAVHLMNKGESPVGALSVVSIALVFALALKRTGNLWLVVGWHAAFDF
                     GETFLFSVPNSGNVFEGHLSNATLQGPVWLTGGTVGPEGSVFSFVTMAVAAFYVHKVF
                     PRKPANSLPAEKRSMALPVA"
     gene            complement(1228..2316)
                     /locus_tag="VFR42_05320"
     CDS             complement(1228..2316)
                     /locus_tag="VFR42_05320"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEU0368605.1"
                     /translation="MRIFAAGAVACALVLFLLSGGCKRSKPGRLSPARIHQITQELAR
                     AAAEATSKDSVVKSRRARGKSAGAGAEEIYVGLRGAGSNMQILKQKLEEIASRHGLAV
                     ASEELNKGGVQMTLRSRGVVTHRIEIKQLAAAANQTSAPPGQARLAILLDDLGNDRSV
                     ADKVFALRVPITISVLPFHAHSEEIAKEAGQHGCEVMLHLPMQSLANESPEQQELRPG
                     LSTDEVRTTVENMLDAVPEADGVNNHQGSQATADNALMNELMLVLKDQGVFYVDSRTT
                     AATVAFDAAKRAGVRTAFRNVPFLDDIQNPEAIKRQLQLAIQGAKWKGEAIAIGHPHS
                     ATLAALREVLPLAEKDGVQLVVVSELVH"
     gene            complement(2399..3631)
                     /locus_tag="VFR42_05325"
     CDS             complement(2399..3631)
                     /locus_tag="VFR42_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0368606.1"
                     /translation="MNRTARIAIIAISIAVFCYAGLGHVLGQTHEERAYKSLTVYGEV
                     LQKIQSDYVDEPNMHLVTAGALHGLLESLDPQSSYLTPREYTEYKQKLASTNPGDTGL
                     TLSKRFGYIIVVSVLPESPAEKTGIRSGDIFESVAGFTTREMSVGQAMNLLRGQPGSG
                     VKVGVIRRGKNEPESVDIVREKLAPAKILSQKTDPDILALRLTSLEAGRADELKTRLQ
                     DAEKQGVHKVVLDLRDCGRGPVSEGLAVARLFISSGNLATLKGQTVSAQTFAAEPSKV
                     VWKGPVAVLTDGTTSGAAEVLASAIGGTHRGDIVGERTFGLASEQKLITIDDGSALIL
                     TVANYYNFDGKSILEEGVTPSEVVRQASAAVDEGADDDADSGQTAQPPAEPRPLSPED
                     AVYKKAVEMFKAPAKKAA"
BASE COUNT          715 a         1199 c         1083 g          899 t
ORIGIN      
        1 cagaaaatcg gcctttccgt ttgatcgccc gaagcgaacg gcaggttggc aaaatcgagg
       61 cagagccggt tggcggagaa ctgaggcgtg aaagggtccg cggacaagtt ggcctccgtg
      121 aggctgcact aactgctaaa agttattctt ctggttaggt ccaagtcaac cactttcgct
      181 gcggcaatga gccggagaaa tcgatcgccg gccagtgctt cgggtcttgt ggtactgttc
      241 atagccaatg gacgaacaca gcactccttc ttcctcccaa ccgagcgaaa cagcaccgcc
      301 acccatccag gtaccgcgtc atccgttcaa cctgtggttt atgaacgagc gcggactacg
      361 ctccggctgg cgaatcgtga tctatctttt ccagattctg gtttttagcc ttgccgtcaa
      421 cttcttgctc ggcagcgttt tgcacttgcc caaaacggcg caacctccca tgtggcagat
      481 gacactgcag gagggacttt cctttctgct tgtgcttctt cccgccgtcg tgatgtcgcg
      541 gatcgagtcg cgctcgcccg gcgactatgg cctcccgctg aacgaattgc tgggcgcgcg
      601 gttctggcat gggtgcgttt tgggactcgt ggaaatttcc gtcctgatcg cgtgcattgc
      661 cgcgtttggg ggctacaaat ttggtgccct ggaccttcac ggttcggcca tcctgcgatg
      721 ggcgctactg tgggcgtttt ttttcctgct ggtcggtctg tttgaagagt ttgcgttccg
      781 cggctatctg caatttacgc tcgcggacgg gatcggcttt tggctcgcgg cctggattct
      841 ctcgctcgga ttcggtgcag tgcacctgat gaataaaggg gagtctcctg tcggcgcttt
      901 gagcgtcgtt agcatcgcgt tggttttcgc gctggcgctt aaacgcacag gaaacctttg
      961 gctggtggtc ggttggcatg cggccttcga cttcggcgaa acatttttgt tctccgttcc
     1021 caacagcggc aacgtttttg aaggccatct ctccaacgct actttgcagg ggccggtgtg
     1081 gctaaccgga ggaacggttg ggccggaagg cagcgtgttc agcttcgtca ccatggccgt
     1141 tgcggcgttt tacgttcaca aggtttttcc ccgcaagcca gccaattcat tgccagcgga
     1201 gaagcggtcg atggctttgc ccgtggccta gtgaaccagt tcggaaacca caactagctg
     1261 tacgccgtcc ttttccgcca ggggaaggac ttcgcgcagc gccgccagcg ttgcggaatg
     1321 cgggtggccg atggcaatcg cttcgccctt ccactttgcc ccctgaatcg cgagttgcaa
     1381 ctgcctcttt atggcttccg ggttttgaat atcatcgaga aaaggaacgt tacgaaacgc
     1441 cgtgcgcacg ccggcgcgct ttgccgcatc gaacgccacg gtggccgcgg tggtgcggct
     1501 gtccacatag aaaactcctt ggtctttcag taccagcatc aattcattca tcagcgcgtt
     1561 gtccgccgtc gcctgcgaac cctgatgatt gttcaccccg tcggcttccg gaaccgcatc
     1621 cagcatgttt tccactgtgg tgcgcacctc gtccgtcgag aggccgggac gcagctcttg
     1681 ctgttcgggc gattcattgg ccagcgattg catcggcagg tgcagcatca cttcgcatcc
     1741 gtgttggccc gcttccttgg cgatttcttc ggagtgagcg tgaaagggca ggaccgaaat
     1801 ggtgatggga acgcgcaacg cgaagacttt gtcggccacg ctgcgatcat tgcctagatc
     1861 gtccagcagg attgccagcc gtgcctgtcc tggcggcgcc gaagtttgat tcgcagctgc
     1921 cgcgagctgc ttgatctcga ttcggtgtgt gacgacgcca cgcgaacgca aggtcatctg
     1981 aacgccgccc ttattcagct cttcggatgc gacggcgagg ccgtgccgcg acgcaatctc
     2041 ttccagcttt tgcttcagga tttgcatatt cgaaccggcg ccgcgcaacc caacgtagat
     2101 ttcctccgcg ccggcgccag ccgatttgcc gcgggcgcgc cgggacttca ccaccgaatc
     2161 tttcgacgtt gcctcggcgg cggctctggc cagctcctga gtaatttgat gaatcctcgc
     2221 cggactcagc cggccaggtt tgctgcgttt gcatccgccg gaaagcaaga acagaacaag
     2281 cgcgcacgcg actgcgcccg ccgcaaaaat tctcatcggc ctgttagcta agccaggtct
     2341 tattgtgtga tggtaactga gttggatcgc gtcgcccgac tgcacggacc ggctatgcct
     2401 acgccgcctt tttcgcggga gccttaaaca tctcgacggc tttcttataa acagcgtcct
     2461 cgggagaaag cggccggggc tctgctggcg gttgcgccgt ctgaccggaa tccgcatcgt
     2521 catccgcgcc ctcatcaact gcggcggagg cttgccgaac cacttcgctt ggcgtcacgc
     2581 cttcttccaa aatcgatttg ccgtcgaagt tgtagtaatt cgccacagtc aagatcagtg
     2641 ccgagccatc gtcaatcgtg atgagcttct gctcggaagc cagcccgaag gtgcgctccc
     2701 cgacgatgtc gccacggtgt gtgccgccaa tcgccgacgc cagcacttcc gccgcgcccg
     2761 acgtcgttcc atcggtcagc acggcaaccg ggcccttcca aaccaccttg ctcggttcag
     2821 cagcaaacgt ctgcgcagaa accgtctggc cctttagcgt cgccagattc ccgctcgaaa
     2881 taaacaagcg ggccacggcc aatccttccg aaaccgggcc acggccgcag tcccgcaaat
     2941 ccaggacaac cttgtgtacg ccctgctttt ccgcatcctg cagacgagtc ttcaactcat
     3001 cggcccgacc agcttccagg ctcgtcagcc gcagcgccaa aatatcggga tcggtctttt
     3061 ggctcaaaat cttcgccggt gccagcttct cgcgcacgat gtccaccgac tccggttcgt
     3121 tctttccgcg gcggatcact ccgactttta ctccactgcc cggctgcccg cgcagcaagt
     3181 tcatcgcctg cccgacagac atctcccgtg tcgtaaatcc tgcgaccgac tcaaaaatat
     3241 cgccgctgcg aatgcccgtc ttttccgcgg gactttccgg aagcaccgaa accacaatga
     3301 tgtagccaaa ccgcttgctc aacgtgaggc ccgtatcgcc gggattcgtg cttgccagct
     3361 tctgtttgta ttccgtgtat tcgcgcggcg taaggtagct cgactgcgga tccaatgact
     3421 ccagcaatcc gtgcagcgct cccgccgtca ccaggtgcat gttcggttcg tccacgtaat
     3481 cggactgaat cttttgcagg acttcgccgt agaccgtgag cgacttgtag gctcgctctt
     3541 cgtgggtctg cccgaggacg tgtcccagtc cggcataaca gaacacggca atcgaaatag
     3601 cgatgatggc gatcctggca gtgcggttca ttctttgccc ttcctttggc gctattaccg
     3661 aagtatagca ccgtacccag ttgggacgcg ccactaaccg gaaaggaagc ctgcgacccc
     3721 tagccgtgac gtgaacgctc tccgctaagt tcgctctaag gcccgaactc attgccacag
     3781 cgaaccttcc atttcgttgt gcgtttacca caccgtccga ccagccgtgt tcttggcggc
     3841 ctgtaagtgt ttgaaaacaa tgcctcaatc aagcgaaagg gagtggagcg gaagat
//
LOCUS       DASTPM010000302         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_846904, whole genome shotgun sequence.
ACCESSION   DASTPM010000302 DASTPM010000000
VERSION     DASTPM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846904"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..820
                     /locus_tag="VFR42_05330"
     CDS             485..820
                     /locus_tag="VFR42_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368607.1"
                     /translation="MESRKYEQRVRDQQVQKQREDDQKTIEQLGGSELAIQTLYATPQ
                     VRRGEAAQVCFGVANAKTVTLEPQSNPVWPSHNLCVDVKPIKTTTYRLTATGADGQSV
                     SQEVTVKVR"
     gene            997..1476
                     /gene="greA"
                     /locus_tag="VFR42_05335"
     CDS             997..1476
                     /gene="greA"
                     /locus_tag="VFR42_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU0368608.1"
                     /translation="MSDPIAKVKQKLQEEIDQLEHELNVDLPKEIAVARAHGDLSENA
                     EYKYAKERQGFVNARIGQLKKRMGDLGMLNLSNIPKDRSGYGSRIVVIDTQRDVRIEY
                     RLVSSEEADVDKGHISTTSPIGRALLNRKVGDEVKVVTPAGAKEFEVVELLTIHQLD"
     gene            1545..1865
                     /locus_tag="VFR42_05340"
     CDS             1545..1865
                     /locus_tag="VFR42_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368609.1"
                     /translation="MVWGSGSALGVLALCGYAVFVCGAVLLWANRMDLPVWVNDEFGA
                     VRRSLVRHAFSVSFHGLREELACKAAPTNFIRRLARHSRRRINRGTILLSVGLMLFLL
                     DFLV"
     gene            1935..2864
                     /locus_tag="VFR42_05345"
     CDS             1935..2864
                     /locus_tag="VFR42_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEU0368610.1"
                     /translation="MRPSLIVHGGAWDIPDEAVTACQEGCRNALAAGWSILSKGGSAL
                     DAVEAAVVVLENDPVFDAGYGSHLNADGIVECDAILMDAATLRAGAAAGLQRIKNPIR
                     LARKIWESCPHMMLLADGAERFAQKSGMALCDPAELVSEAERAAYLRCREDQHAAIHH
                     RGHEQGTVGAVAMDRAGRLFAATSTGGTCCKLPGRVGDSPLIGCGCYADCEAGGISCT
                     GYGEAIMKIVMAKTATDYLRREPLANENEVSFATRAAQDAVDLLARRGKGTGGLILLD
                     RHGNPAFAFNTPRMAYGYVAADASFFTAVESRL"
     gene            2914..>3635
                     /locus_tag="VFR42_05350"
     CDS             2914..>3635
                     /locus_tag="VFR42_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0368611.1"
                     /translation="MPLLYNRGPLPMPMEIDPAQAHSIALVIEEAGAFPEMKRGLQPL
                     FRARLANNENAIKDAVEDASLQAMLLDLDSIGDGASDGVEVLQEIRAIRADLVLVAMT
                     RSREHTIPLRASQAGADEFVHAPVNFEELQGLIARAIEKRALELEGRRIVEQAERRSA
                     LFSLIGASAAMQKLYQTIQAVADTNTSVVLRGESGTGKELIAKAIVQASDRVAQPYIC
                     LNCSALPESLIESELFGYEKGA"
BASE COUNT          761 a         1040 c         1071 g          763 t
ORIGIN      
        1 atgagcacag cagtacccac atgactaaca tggtaaaaac acgtttcact tcgagccccc
       61 caaatttaaa gttcggttga tgaagctatc gcttcgggaa caaaccgcgc ggtcgagacc
      121 tgtggcggcg gactggagtt ccttagaaag cttagtaagt gtgtcgccta gtgatatgga
      181 aattgtgatg agaagttgaa ttctctgtga caatgcgatc gttgcgaaag gggcttttta
      241 ctgcatctgc tctcattcaa ggtgctctag gagtttcacg gcgaccagcg aaatctcgct
      301 ctttaccaat tgccggttgg catttatgtt ggaacaagat tttgcgggac atggaaagtt
      361 tatgctcagc gagaaaccga ctcaagttcc gagaaagacg gacctaaaac gtccgctggt
      421 ctattcgtcg ttactccttg ttgttgtggc ggcctatgta agctgggttt tctactcgcg
      481 ccatatggaa agtcgcaaat atgagcagcg agtccgcgac caacaagtgc agaaacagcg
      541 ggaagacgac cagaaaacga ttgagcaact cggagggagc gagctggcga tccagaccct
      601 gtacgccacg ccgcaggtcc gccggggtga agctgcgcaa gtgtgctttg gcgtggctaa
      661 cgccaagacc gtaactctcg agccgcagag caaccctgtt tggccttcgc acaacctctg
      721 cgttgacgta aagccaataa aaacaactac ttataggctt acagccaccg gagcggatgg
      781 ccaatcggtg agccaagaag tcacggtgaa ggttcgctga cagttcgtcg aaccggcccg
      841 gaccggccgg agctaaatcg gctcttgtcc acccaattta gtcgcatcta tacttatgtc
      901 agcgcggggg cggccttata tcatccttcc cccgcgaatc tttttttatg tccgggtgtt
      961 tcgaacgaac ccgcgaaacc catttggtga aggcgtatgt ctgaccctat cgcaaaggtt
     1021 aagcagaagc tgcaagaaga aatcgaccag ctcgaacacg aactgaacgt cgatctgcca
     1081 aaagagattg cggtggcgcg agcccacggt gacctctccg agaacgccga gtacaagtac
     1141 gccaaggagc gccagggctt cgtcaatgcc cggatcggtc aactcaagaa gcgcatgggc
     1201 gaccttggca tgctgaatct cagcaatatt cccaaagacc gctccggcta tgggtcccgt
     1261 attgtggtta ttgacaccca gcgcgatgtc cgtatcgaat accgcctggt cagcagtgaa
     1321 gaggccgacg tggacaaggg acatatttcc acgacctcac cgatcggccg cgcgctgctg
     1381 aaccgcaaag tcggcgatga agtcaaggtt gtcaccccgg ccggcgcgaa agaatttgaa
     1441 gtcgttgagc tgctcaccat ccaccagctt gactagtcgc cgaaaccagc caacccagcc
     1501 agccgtttca ttcgccagtg catctcaagc tctcgagagg ttagatggtc tggggttctg
     1561 gttccgcgct cggcgttctg gccctttgcg gctacgcggt gttcgtctgt ggggcggtgc
     1621 tgctgtgggc gaatcgcatg gacctgccgg tttgggtcaa cgacgagttt ggggcggtgc
     1681 ggcggagcct ggtgcgccac gcgttttctg tttcgttcca cggcttacgc gaagaacttg
     1741 cttgtaaggc tgcacccacc aatttcatcc ggcgacttgc tcgccactcg cgccgccgta
     1801 ttaaccgagg caccatcctt ctcagcgttg ggctgatgct gttcctgctc gacttcctgg
     1861 tgtagacgct ttacgccaag tgccttcctg ttcttggcga ttacccgcgc tggtgccgta
     1921 caatttcttt cgctatgcga ccctctttga tcgttcacgg cggggcttgg gacattcccg
     1981 atgaagctgt aactgcatgc caggaaggct gccgcaatgc tctggccgcc ggctggtcga
     2041 ttctttcgaa aggaggttcg gctctggacg ccgttgaggc cgcagtcgtt gtgctggaga
     2101 acgatccggt gtttgacgca ggttacgggt cccacctcaa cgcggacggc atagtggaat
     2161 gcgacgccat cctgatggac gccgcaacgc ttcgcgccgg agctgccgcc ggattacagc
     2221 gcatcaagaa tccgattcga ctggcgcgca agatttggga gtcctgtccg cacatgatgc
     2281 tgttagcgga cggtgctgaa agattcgcgc agaagtccgg catggcactg tgcgaccctg
     2341 cggagcttgt cagcgaagcc gaacgagcgg cttatctgcg ttgccgcgaa gaccagcacg
     2401 ccgccattca ccatcgcggc cacgaacagg gtacggtggg cgctgtggct atggaccgcg
     2461 ccggccggct cttcgccgcc acttccactg gcggcacttg ctgcaagctt cccgggcgcg
     2521 tcggcgactc gcctctgatt gggtgtggct gttacgcgga ctgcgaagcg ggcggcatct
     2581 cctgcactgg ctacggcgag gccatcatga aaatcgtaat ggccaagacg gccaccgatt
     2641 atctgcggcg cgaaccgctg gccaacgaga atgaggtttc ttttgcgacc cgcgccgcgc
     2701 aggacgcagt agaccttctg gcccgccgcg gcaagggcac tggtggtctc attcttcttg
     2761 atcgccatgg caaccccgca ttcgccttca acacgcccag gatggcctac ggctatgtgg
     2821 ctgcggatgc ctcattcttc accgcggtcg agtcgcgtct ctgaatctgc gcgcggtcaa
     2881 gatgaaacat gcgcggcttc tgctgacaga catgtgccgc tcctttataa tcgcggccca
     2941 ctgcccatgc cgatggaaat cgatcccgcc caagcacatt cgattgcgct ggtaatagag
     3001 gaagccggcg cattcccgga aatgaagcgc gggttgcagc cgctgtttcg cgccaggctc
     3061 gcgaacaacg aaaacgccat caaggacgcg gtagaagacg ccagcctgca ggcgatgctg
     3121 ttggatctgg acagcatcgg cgacggagcc agcgacggcg ttgaggtgtt gcaggaaatt
     3181 cgcgcgattc gcgccgatct ggtcctggtg gctatgacgc gttcgcgcga acacaccatc
     3241 cctttgcggg cgagccaggc gggagcggac gagttcgtac atgctccggt caatttcgaa
     3301 gaattgcagg gcctgatcgc gcgcgccatc gaaaaacgcg ccctcgaatt ggaaggccgg
     3361 cgcatcgtcg aacaggccga acggcggtcc gcgctctttt cactgattgg cgccagcgcc
     3421 gccatgcaaa agctgtacca gacgattcag gccgtggcgg atacgaacac ctcggtggtg
     3481 ctgcgcggcg aaagcggaac gggcaaggaa ctcatcgcga aagcgattgt gcaggccagc
     3541 gatcgcgtcg cgcagcccta catctgcctg aattgctccg ccttgcctga atcgctgatt
     3601 gaatcagaac tttttggcta cgagaaagga gcgtt
//
LOCUS       DASTPM010000303         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_126367, whole genome shotgun sequence.
ACCESSION   DASTPM010000303 DASTPM010000000
VERSION     DASTPM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126367"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFR42_05355"
     CDS             <1..925
                     /locus_tag="VFR42_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0368612.1"
                     /translation="GRPAQGSTAPAKTRILALQNSFHGRTFGAVSVTATEKYRLPFAP
                     LVPGVEFVAFNDVADLEAKLDDTVCAVILETIQGEAGIYPVSEEFWERARSLATMHGA
                     LLIADEIQCGLGRTGRYFAYQKFPSKPDIAVVAKPLAAGLPLGAILTNEAVASRVSPG
                     LHGTTFGGGPMICSVALEFLEIIESENLLKNARERGEELRAGLKKLQAKYDFIKEIRG
                     EGCMFGVELSIEGGPVVAEALQQGLLINCTHEFTIRLLPPFLISKGQVREFLKVFENV
                     LFQTPRVAPAAETSEKKAPSRVARVATSVSR"
     gene            928..1875
                     /gene="argF"
                     /locus_tag="VFR42_05360"
     CDS             928..1875
                     /gene="argF"
                     /locus_tag="VFR42_05360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0368613.1"
                     /translation="MATTTLAIPAQETNDLLTGAEWSPAHSRELLAITSDIKANPGRY
                     AETLRGRFLALIFEKPSLRTRVTFEVGIQSMGGHVVFLDHTQARLGERESIADVARNL
                     ERWVQGIVARVYEQRVLDEMAATTEIPIINALSDRFHPCQALADYFTLEEKFGSLKGL
                     KLAYVGDGNNVCHSLLYLGARLGVNLRVATPANYAPDADVVADSRRVAKETRAKIELF
                     SDAREAVEDVQAVYTDSWTSMGFEAEEKVRSEVFKPYQVNRRLMALAERGAAFMHCLP
                     AHRGYEVTSDVLDGAQSVVLDQSENRMYVQKAILHTLFR"
     gene            1936..>2827
                     /locus_tag="VFR42_05365"
     CDS             1936..>2827
                     /locus_tag="VFR42_05365"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU0368614.1"
                     /translation="MKTAAPKPKKVVLAYSGGLDTSIIIPWLKENYGCEVIAMIGDVG
                     QQEDLEAARKKALATGASTAYVEDLREEFIRDYIWPTLRAGAVYEHTYLLGTSMARPV
                     LGKRQAELALQLGADGLSHGCTGKGNDQVRFELAYKAIAPNLQIIAPWREWDIDSRED
                     AIAYAEKHNVPIEQSKKNIYSRDRNIWHLSHEGGELEDPANAPSEAMWQWTVSPEKAP
                     DKPEEVEIGFEAGTPVSVNGAKLGPIDLLNSLNDIAARHGVGRTDLVENRLVGMKSRG
                     AYETPGGTLLVTAHRELEALT"
BASE COUNT          609 a          769 c          906 g          543 t
ORIGIN      
        1 cgggcggccc gcgcagggtt ccacggcgcc ggcaaaaacc cgcatcctgg ccttgcagaa
       61 ttccttccat ggcagaactt ttggcgcggt tagcgtcacg gccacggaaa aatatcgttt
      121 gccgttcgct ccgctggtgc ctggagtaga gtttgttgcg tttaacgacg tggcggacct
      181 ggaagcaaaa ttggacgaca cggtctgcgc ggtcattctg gagacgattc aaggcgaagc
      241 cggaatttat ccggtaagtg aagagttttg ggagcgcgcg cggtcactgg ccacgatgca
      301 tggtgcgttg ctgattgccg acgagattca atgcgggttg ggccgcacgg gacgatattt
      361 cgcgtatcag aaatttccgt ccaagccgga tattgccgtc gtcgctaagc cgctggcggc
      421 ggggttgccg cttggcgcaa ttttgacgaa tgaagccgtg gcgtcgcggg tctcgcccgg
      481 attgcacggc acgactttcg gcggcggccc gatgatctgc tcggtagcgt tggaatttct
      541 ggaaatcatt gaaagcgaga acctgctgaa gaatgcgcgc gagcgtggcg aggaacttcg
      601 cgccgggctg aagaagctgc aagccaagta cgacttcatc aaggaaattc gcggcgaagg
      661 ctgcatgttc ggcgtggagc tctccatcga aggcggtccg gtcgtcgcgg aagcgctgca
      721 gcagggactg ctcatcaatt gcacgcacga atttacgatt cgcctgctgc cgccgttctt
      781 gatcagcaaa gggcaagtgc gcgaatttct caaggtattt gaaaacgtgt tgtttcaaac
      841 cccgcgagtg gcgccggcgg cggagacctc tgaaaagaag gctccgtcgc gggtggccag
      901 ggtcgcgact tccgtttcga ggtgaaaatg gcaacgacta cactggcaat tccggcgcag
      961 gaaaccaacg atctcctcac cggagcggaa tggagcccgg cgcattcgcg ggagctgctc
     1021 gcgatcactt cggacatcaa agccaatccg ggacgctacg ccgaaacgct gcgcggcaga
     1081 tttctggcgc tgatttttga gaagccctcg ctgcgcacgc gtgtcacgtt cgaggtgggc
     1141 atccagagca tgggcggcca cgtggtgttt ctggaccaca cgcaggcgcg gctcggcgag
     1201 cgcgagtcca tcgcggacgt ggcgcgcaac ctggagcgct gggtgcaagg catcgtggcg
     1261 cgcgtatacg agcagcgcgt gctcgacgaa atggcggcga cgacggagat tccgatcatc
     1321 aatgcgctgt ccgatcggtt tcatccgtgc caggcgctgg ctgattattt cactctggaa
     1381 gaaaagttcg gctcactgaa gggtctgaag ctggcgtacg tcggcgacgg caacaacgtt
     1441 tgccattcgc tgctctacct cggagcgcgg ctgggcgtga atttgcgcgt ggcgacgccc
     1501 gcgaactatg ctccggatgc ggacgtggtt gcggattcgc ggcgggtggc gaaggaaacc
     1561 agggctaaga tcgaattgtt ctccgatgcg cgcgaagccg tcgaagacgt gcaggcggtg
     1621 tacaccgatt cgtggaccag catgggcttt gaggcggaag aaaaggtgcg cagcgaggtg
     1681 ttcaagccat atcaggtgaa tcgcaggctg atggcgctgg ccgaacgcgg tgcggcgttc
     1741 atgcactgtc tgccggcgca tcgcggctac gaagtaacgt ccgacgtgct cgacggcgcg
     1801 cagtccgttg tgctcgatca atccgaaaat cgcatgtacg tgcagaaggc gattctgcat
     1861 accctgttcc gctagaaact tttcgcagga gtctcggcgc acttcgtgtg ccaacaacaa
     1921 ccagaaagag gaatcgtgaa aacagcagca cccaaaccaa agaaagtagt tcttgcttac
     1981 tccggaggat tggacacctc cattattatt ccgtggctca aggaaaacta cggttgcgaa
     2041 gtgatcgcca tgatcggcga cgtcggccag caggaagatc tggaagcggc gcgcaagaag
     2101 gcgctggcta cgggagcttc caccgcatac gtggaagatc tgcgcgaaga attcattcgc
     2161 gattatatct ggccgacgct gcgcgctggc gcggtgtacg aacatacata tttgctgggt
     2221 acttcgatgg cgcggccagt attggggaag cggcaggctg aactggcatt gcaacttggc
     2281 gcggatggcc tttcgcacgg ttgcaccggc aaaggtaacg accaggtgcg cttcgaactg
     2341 gcctacaagg cgattgcgcc gaatctgcaa atcattgcgc cctggcgcga atgggacatt
     2401 gattcccgcg aagacgccat cgcctacgcc gaaaagcaca acgtgcccat cgagcagagc
     2461 aagaaaaata tctatagccg cgaccgcaac atctggcact tgagccatga aggtggcgaa
     2521 ctggaagatc cggccaacgc cccgagcgaa gccatgtggc aatggacggt ctcgccggaa
     2581 aaggcgcccg acaaacccga agaagtggag attggctttg aagcaggcac gccggtttca
     2641 gtgaacggcg cgaagctcgg gccgatcgac ttgctgaatt cgttgaacga tattgcagcg
     2701 cgtcacggtg ttggacgcac ggacttggtg gaaaatcgcc tggtcggcat gaagtctcgc
     2761 ggcgcctacg aaactcccgg cggcacattg ctcgtcaccg cgcatcgcga actcgaagcc
     2821 ctcaccg
//
LOCUS       DASTPM010000304         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_154732, whole genome shotgun sequence.
ACCESSION   DASTPM010000304 DASTPM010000000
VERSION     DASTPM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154732"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..973
                     /locus_tag="VFR42_05370"
     CDS             80..973
                     /locus_tag="VFR42_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0368615.1"
                     /translation="MGRTAGVIVVQRIAAGLVENNQVTGAMKLFPEKGESHDALLSMP
                     ADSIAVNIAELVSWLANGQTLSALGVGFPGIIRDGVVEESPNLQQIKGLRLQLALEQI
                     LAASGLSVPVRLYNDADATAAGIAATRGVLHKLNRVWTLGNGVGFGRYPWAAGTWEGG
                     HSVVTLDPKEQFCGCGGRGHLEGILGYRAMRMRFLDLEPEEVFANAKTGDPRCVEFVN
                     RCHRALAAATATSIHMDGPGPFYLTGPNAKWIDVGLLDRLVHEMVKMSPLQGSRIEVI
                     PPNDDLGIIGAAVNAERATTT"
     gene            complement(988..1461)
                     /locus_tag="VFR42_05375"
     CDS             complement(988..1461)
                     /locus_tag="VFR42_05375"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0368616.1"
                     /translation="MKKILLCWVAMLVWGGIVRAQEPDEPKPNPEMIRQELINLEKEN
                     ARAIKLHNPTFFKSAYSEDFTGVTRYGEVLNKAAIIREIASMPQEFDSVISSDAQVRM
                     FRDTASVLSMRSEVGHANGKKFYNQYRILRMYINTGSGWKVVSQLETMLSGESGH"
     gene            1670..>2145
                     /locus_tag="VFR42_05380"
     CDS             1670..>2145
                     /locus_tag="VFR42_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0368617.1"
                     /translation="MQVNGLSGEPNYSRRLFLGRVSQWSALLAAYPLIPLPGLAETLA
                     SDKRIAQTPLVDKGFAAVRNVGDGLYATISDTSKGLQTMCNGGFLAGKDAALLVEGFV
                     SPAGAAFQHETLRSVSKAPVMGAIDTHYHFDHSTGNSYYGANGIALWAHANTSRRI"
BASE COUNT          437 a          613 c          662 g          433 t
ORIGIN      
        1 cgcggatcac aaccgcggca agggaacgaa ctagctggaa ctcgccggcg gcagtcggcc
       61 gggattacgg gcggaacata tgggcaggac agccggagtc atcgtggtac agcgaatcgc
      121 cgcgggcctg gtggaaaaca accaggtcac cggagcgatg aagctcttcc cagaaaaggg
      181 cgaatcgcat gacgcgctgc tctccatgcc ggccgatagc atcgcggtca atatcgcgga
      241 actcgtctcg tggctcgcga atggccagac gctgagcgcg ctgggcgttg gttttcccgg
      301 gatcatccgc gacggcgtgg tggaagaatc gcccaacctg caacagatca agggccttcg
      361 gctgcagctt gccctggaac aaatcctcgc ggcgagcggc ctcagcgtgc cggttcgtct
      421 ttacaatgac gcggacgcta cggcggccgg tatcgctgcc acgcgcggcg tactgcacaa
      481 gctgaaccgc gtttggacct tgggcaacgg cgtcggcttt gggcgctatc cgtgggcggc
      541 gggaacgtgg gagggcggcc actcagtggt tacgctcgat cccaaggaac aattttgcgg
      601 atgcggcggc cgcggccacc tggaaggtat tctgggttat cgcgcgatgc gcatgcggtt
      661 tctggatctc gagccggagg aagtgttcgc caacgccaag actggcgatc ctcgctgcgt
      721 ggaatttgta aaccggtgcc atcgcgcact tgcggctgca acggcgacca gcattcacat
      781 ggacggcccc ggcccgttct atctgactgg gccaaacgca aagtggatcg acgtcggcct
      841 gctggatcgc ctggtgcacg aaatggtcaa gatgagcccg ctgcagggca gccgcatcga
      901 ggtgataccg ccgaatgacg acttggggat aattggcgcg gcggtaaacg ccgagcgcgc
      961 aaccacgacg tagcgcgctt cgcagcctta gtgtccggat tcgccggaga gcatcgtctc
     1021 caactgtgag accaccttcc agccgcttcc tgtattgata tacatcctta agatccggta
     1081 ctggttgtag aacttcttcc cgttagcgtg gccgacttcc gatcgcatgc tgagcaccga
     1141 agcggtatcg cgaaacatcc gcacctgggc atcgctgctg atcacggaat cgaattcctg
     1201 gggcatgctc gctatttcgc ggatgatggc ggctttattc aacacttctc cgtagcgcgt
     1261 gactcccgta aagtcctcag aatacgccga cttgaaaaag gtgggattgt gcagcttgat
     1321 ggcgcgggcg ttctcttttt ccaggttgat cagctcttgc cggatcattt ccgggttggg
     1381 tttcggctca tcaggctcct gagcacggac aatccctccc cagacaagca tcgcgaccca
     1441 gcacaagaga atcttcttca cggtcgttta cctcaaggcc ttgcaacacg atttgagaga
     1501 gaaaagaagc tgaatccagt atagttccgg gcggtggcgg ttggcgcggt tgcgcgtaac
     1561 cctgcaccca aacaggcatc caacggggac tcttagtttt aagaagtgga acgatgtcgg
     1621 tcgtgcgcga atgcgcgatt agagctgtct ttactactga ggaggaatca tgcaggttaa
     1681 tggtttgagt ggcgagccga actattcgcg gcgtctattt ttgggccgcg tttcgcaatg
     1741 gagcgcgttg ctcgcggcgt acccgctcat ccctttgccg ggattggcgg agacgctcgc
     1801 gagcgacaag cggattgcgc aaacgccgct ggtggacaaa ggatttgcgg cggtgcgcaa
     1861 cgtcggtgat ggcctatacg caaccatttc ggacacgtcg aagggcttgc agaccatgtg
     1921 caacggcggt ttcctggccg gcaaagacgc tgcgctgtta gtcgaaggat ttgtcagccc
     1981 tgccggcgcc gcatttcaac acgaaacctt gcgctcggtt agcaaggctc ccgtcatggg
     2041 cgccatcgac acgcactacc acttcgacca cagcacggga aattcctact atggagcaaa
     2101 cgggattgcg ctctgggccc acgcaaacac atcgcggcga atctt
//
LOCUS       DASTPM010000305         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_760460, whole genome shotgun sequence.
ACCESSION   DASTPM010000305 DASTPM010000000
VERSION     DASTPM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760460"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VFR42_05385"
     CDS             complement(<1..1292)
                     /locus_tag="VFR42_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0368618.1"
                     /translation="MSEVKKMSRRAFARNAAAVGAGLTIVPRHVLGRGMTPPSDLLNV
                     AGVGIGGMGRTNLVNLASQNIVALCDVDWGYADKGFERLGTDIENLQRRMDGPDPELQ
                     PRRPQRPFDRNEARLRLAGMQRLRDVHLPKAKRYVDYREMLDKQKDIEAVLVATADHM
                     HAPIAVAAMEVGKHVYVQKPLTWSVAESRQLARLAKERRVATQMGNQGHSLDDARKAV
                     EYVWAGAIGEVREVHIWTNRPLGFWPQGVPRPAAAKEPVDTLRWNMNGVEARLAAAEL
                     GSGYPVPEKLNWDLFLGVAPKVEYHPIYHPFNWRGWADWGMGPIGDMGAHLIDHSMWA
                     LKLGYPTTIETISTPFNGASYPSATTTYYEFPSREVDGKTMPAVKLTWYDGGLMPPKP
                     TELGDEELDKGGGALLIGSKGKLLHETYGLNPRLLPKSL"
     gene            1414..1701
                     /locus_tag="VFR42_05390"
     CDS             1414..1701
                     /locus_tag="VFR42_05390"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEU0368619.1"
                     /translation="MYIHMFAFRLKPGVTEEQKDRMIREIGALQKQIPGILETAVGKN
                     DSPRGQGYEIGGAMKFPDKAALEAYNVHPVHQALLKWLVPLIEAIEVDFPA"
     gene            complement(1705..>2240)
                     /locus_tag="VFR42_05395"
     CDS             complement(1705..>2240)
                     /locus_tag="VFR42_05395"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HEU0368620.1"
                     /translation="NRVDQETGWRYMGIDLSPAPLKEVSIGAAIEELIQQPFGSPGTL
                     TAAGTITTAVRNVPVKQTGYNGLMLPILEDSRLAQRWSEGQVSLEGLLSYSAVCGTGL
                     DTVPLPGAVTIEQLDMILADVATLAVKWHKPLSARLLPVKGKGPGEMTEFDDPFLVNS
                     TIQPFAQNAATGGNAPK"
BASE COUNT          419 a          735 c          592 g          494 t
ORIGIN      
        1 agcgatttgg gcagcagccg cggattcaat ccgtaagttt cgtgcagcag cttgcctttg
       61 cttccgatca gcagcgcgcc gccgcccttg tccagttctt cgtcgccaag ttctgtaggc
      121 ttcggcggca tcaatccgcc gtcgtaccac gtcaacttca ccgcaggcat cgtctttccg
      181 tcaacctcgc gcgacgggaa ttcgtagtac gtcgtcgtcg cgctcggata cgaagctcca
      241 ttgaacggcg ttgaaattgt ctcaatcgtt gtaggatagc ccagctttag cgcccacatc
      301 gaatgatcga tcaagtgcgc gcccatatca ccaatcgggc ccatgcccca atccgcccag
      361 ccgcgccaat tgaacggatg atagatcggg tgatactcca ctttcggcgc cactcccaaa
      421 aacaggtccc agttcaattt ctccggcacc ggataaccac ttcccagttc tgcggctgcg
      481 aggcgtgctt ccactccgtt catgttccag cgcagcgtgt ccaccggctc cttcgccgcg
      541 gctggccgcg gtactccttg cggccagaat cccagcggcc ggttggtcca gatgtgaact
      601 tcgcgcactt cgccaatcgc gcctgcccac acgtattcga ctgctttgcg cgcgtcgtcc
      661 agcgaatggc cttggttgcc catctgtgta gcgactctcc tctctttcgc cagccgcgcc
      721 agttgccgtg actccgctac gctccaggta agcggcttct gcacgtatac gtgcttgccc
      781 acttccatcg ccgctacggc gatcggcgcg tgcatgtggt ccgctgtcgc caccagcacc
      841 gcctcgatgt ctttctgctt gtccaacatc tcgcggtagt ccacataccg cttggctttc
      901 ggcaggtgca catctctcag ccgctgcatt ccggccagcc gcaacctcgc ttcattgcgg
      961 tcaaacggcc gctgtggtct tcgcggttgc agctccggat cgggaccatc catccgcctc
     1021 tgcaaattct caatgtcggt tcccagccgt tcgaatccct tgtccgcata gccccaatcc
     1081 acatcgcaca gcgccacgat attctggcta gcaaggttca ccaggttggt gcggcccatt
     1141 ccgccgatgc ccacgcccgc aacgttcagc aagtcgctcg gcggcgtcat cccccgcccc
     1201 agcacgtgcc gcggaacgat cgtcaatccg gcgcctaccg ccgccgcatt tctcgcgaac
     1261 gcccgccgcg acatcttctt cacttcgctc attgcttccc ccaggtcgtt tcttgccttg
     1321 ttcgccgcaa aatcgcagcg ttcattctta ttgacacgcg gtggccgcgt caccaaaaat
     1381 cttttcgtaa attttcggag ccagcaacca gaaatgtata tccacatgtt tgcgtttcgt
     1441 ttgaagccgg gagttaccga agagcagaaa gaccgcatga ttcgggaaat cggcgcactg
     1501 caaaaacaaa ttccgggcat cctggaaacc gcggtcggca aaaatgattc gccgcgcggc
     1561 cagggctacg aaatcggcgg cgccatgaaa tttccggaca aagccgcgct ggaagcctac
     1621 aacgtccacc ccgtgcatca ggctttgctg aagtggctcg tcccgctcat cgaagccatc
     1681 gaagtcgatt tccccgctta gaaattattt gggagcattc ccgcccgtcg cggcgttctg
     1741 cgcgaagggc tgaatcgttg agttaacgag gaacggatcg tcaaactcgg tcatttcacc
     1801 gggacctttg ccctttaccg gaagcagccg cgcggagagc ggcttatgcc acttcaccgc
     1861 cagcgtggcc acgtcggcga gaatcatgtc gagctgctcg atcgtcacgg cgccgggtag
     1921 cggcacagtg tccagtccgg tgccgcagac cgccgaataa gagagcagac cttctagtga
     1981 gacttggcct tcgctccaac gctgcgccag ccgggagtct tccagaatcg gcagcatcaa
     2041 tccgttgtag ccggtctgtt tgacgggaac atttcttacg gcggtggtga tagtgcccgc
     2101 agcggtcaga gttccgggtg agccaaacgg ctgctgaatt aattcttcga ttgcagcgcc
     2161 gatggaaact tctttgagcg gggcgggcga aaggtcaatg cccatgtagc gccagccggt
     2221 ttcctgatcc acgcgattgg
//
LOCUS       DASTPM010000306         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_97203, whole genome shotgun sequence.
ACCESSION   DASTPM010000306 DASTPM010000000
VERSION     DASTPM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97203"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFR42_05400"
     CDS             <1..190
                     /locus_tag="VFR42_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368621.1"
                     /translation="DLALLDLAWPCAVAVKVLRNIRTKKPELPLPIRYGLRPTGRSAR
                     CLTGRLFASYEAPAIKSR"
     gene            complement(286..2346)
                     /locus_tag="VFR42_05405"
     CDS             complement(286..2346)
                     /locus_tag="VFR42_05405"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU0368622.1"
                     /translation="MNSKWFGGVALALAAAVASPAQQWRQVGPAGGTVISLEADPRDV
                     NKIYLGTSDGHVFFSSDEGQHWNLLSRIGSGQDDVITHILVDPRDSKRLYASTWTLYS
                     GGGGVYRSDDSGRSWRLIGLAKETVRALAQSPTNPKLFVAGSLTGVYRSNDDGNTWER
                     ITPANHEDLRNFDSLAFDTKDSNTIYAGTYHLPWKTTDGGKNWTSIKAGMIDDSDVMT
                     VIVDPANPSNVHATACSGIYRSVDAGQNWKRYGGIPFVYRRTQLIRQDPQHPEVLYAG
                     TTSGLWKTTNEGVDQWKRLTPLDWVVNAILIDPRNPQRIIIGTEREGVQISENGGATF
                     SAGNAGFHHQHILDVAIDREHPDHALTVLTFDTNAFLATKNGGASWTTLGPGLKRTDL
                     RHVYAMPNGWWASLANGGLMKYDEATNKWVKAGLFVAEAPAAVAQTSTKTTAKGKKAA
                     PAPAAKKPLPKKAVPQVLTFQVNDLTYGGSKWFAATTGGVLVSTDKGATWKQAASEAL
                     VKLPAQSLEASSDSSQVWAVSQKNLLYSADGGAHWEAKELSFASAGNLRLRRMDDDNL
                     FITTNMGLYTSRDSGRNWSRADIRDLQFQDAAGAGTARLVALQRRGLVASFDSGKTWQ
                     RINDPLAEGYFPLVRANNSGSLFAVSATEGLFTLEAGARSASEAVGGSSLMMLDTNGA
                     QKPR"
BASE COUNT          467 a          782 c          706 g          533 t
ORIGIN      
        1 cgatctcgcg ctgctcgatc ttgcctggcc ctgcgcggtt gccgtgaaag tgctccgcaa
       61 catccgcact aaaaagccgg agctaccgct ccctattcgt tacggcctcc gcccgacggg
      121 gaggagcgcg cgctgtctga ccggccgctt attcgcatcg tacgaggcac cggctatcaa
      181 atcgcggtga acgggcaggt ttcctgacat tgtgaagccg ccgaaaaaaa gcaaaagcgc
      241 cccgaaattt ccggggcgcc ttcgcgtctg tagaaagaag ctaacttagc ggggcttctg
      301 cgctccattc gtatcgagca tcattagcga tgaacctccc accgcctcgc tcgccgaacg
      361 tgcgcccgct tccagcgtga agagtccttc cgtcgccgat accgcaaaca gtgagccgct
      421 gttgttcgcg cggacgagtg ggaagtaacc ttccgccagt ggatcgttga tgcgctgcca
      481 cgtcttgccg ctgtcgaacg acgccaccaa tccgcgccgc tgcaaagcta ccagccgcgc
      541 agtgcccgcg cccgccgcat cctggaactg caaatcgcgg atgtccgcac ggctccagtt
      601 gcgtcccgag tcgcgcgaag tgtataggcc catgttggtc gtgataaaaa gattgtcgtc
      661 atccatgcgg cgcaaacgca ggttgccggc cgaggcgaac gatagttcct tcgcttccca
      721 gtgcgctcca ccgtccgctg agtagagaag attcttctgc gacacggccc atacttgcga
      781 gctgtcgctg gaggcttcga gcgattgcgc cggcagcttc accaacgcct cggacgccgc
      841 ttgcttccat gtcgcgcctt tatcggtcga aaccaatacg cctccggtcg tcgctgcgaa
      901 ccacttgctg ccgccgtagg tcaggtcatt cacctggaac gtcagcactt gcggaacagc
      961 cttcttgggc aacggcttct tcgcagccgg ggctggcgca gccttctttc ccttcgccgt
     1021 tgttttggtg ctcgtttgcg caaccgcagc cggcgcttcc gcaacaaaca aaccagcttt
     1081 cacccactta ttggtcgcct cgtcgtactt catcaaaccg ccgttggcca gcgatgccca
     1141 ccagccgttc ggcatggcat acacgtgacg cagatcggta cgcttaagcc ccggaccgag
     1201 cgtcgtccag ctcgctccgc catttttggt ggcaagaaat gcgttggtat cgaatgttag
     1261 taccgtcagc gcatggtccg gatgctcgcg atcaatcgcc acatcgagaa tgtgctggtg
     1321 atggaaaccg gcgttcccgg cgctaaacgt cgcaccgcca ttttcgctga tctgtacgcc
     1381 ttcgcgttcc gtaccgatga tgattcgctg cggattgcgt ggatcgatca gaatcgcgtt
     1441 cacgacccag tccagcggag tcaaccgctt ccactggtcg acgccttcat tcgtggtctt
     1501 ccagagcccg ctagtcgtac cggcatacaa aacttccgga tgctgcgggt cttgccgaat
     1561 cagctgcgtg cggcgatata cgaacggaat gccgccgtag cgcttccagt tctggcccgc
     1621 atccacgctg cggtaaatac cggaacatgc ggtggcgtgc acgttcgagg ggttcgcagg
     1681 atcgacgatg accgtcatca cgtcggaatc atcaatcatc ccggctttga tcgaggtcca
     1741 gttcttgccg ccgtccgtgg ttttccaagg caagtggtaa gtgcctgcat aaatcgtgtt
     1801 ggaatctttc gtatcgaatg ccagcgaatc gaagttccgc aaatcttcgt ggttcgcggg
     1861 agtaatgcgt tcccacgtat tgccgtcgtc attcgagcgg taaacaccgg tcagcgaacc
     1921 ggccacaaac agctttggat ttgtcggcga ttgcgccagc gcgcgcaccg tttctttggc
     1981 aagtccgatc agccgccagc tgcggcccga atcgtcgctg cgataaacac cgccgccgcc
     2041 cgagtaaagc gtccaggtgc tggcgtaaag gcgcttcgaa tcgcgcgggt ccacaaggat
     2101 gtgagtgatg acgtcgtcct ggccggagcc gatgcggctc agcaggttcc agtgttggcc
     2161 ctcgtcgctt gagaaaaaga cgtgcccgtc ggaagtgccg agataaatct tgtttacgtc
     2221 gcgcgggtcg gcctcgagcg agatcaccgt cccacctgcc ggtcccacct gtctccattg
     2281 ctgcgccggg ctggccactg cggctgccag ggcgagcgcc actccgccaa accacttcga
     2341 gttcatacgc atgcattctc ccagaggggg cttgtgccca caagccagga gtgtaggtta
     2401 tcagctggat tatccgcgcc tgtcctaagg gccagattcg gggcagttta gttatagttc
     2461 tagtactcgc gatggccgac tgtcagga
//
LOCUS       DASTPM010000307         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1423757, whole genome shotgun sequence.
ACCESSION   DASTPM010000307 DASTPM010000000
VERSION     DASTPM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423757"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2590)
                     /locus_tag="VFR42_05410"
     CDS             complement(<1..>2590)
                     /locus_tag="VFR42_05410"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0368623.1"
                     /translation="SYRGTLLDRACKTAVELQIEYGNSHGVPWGVSESGFNQGDAHQI
                     YQYRAFGVPGLGLKRGLAEDLVIAPYATALALLVAPREASENLQRLARDGREGAYGFY
                     EAVDYTPSRLPPDESSVTVRSYMAHHQGMSLLALVSLLRDLPMQRRFMARPLLKSADL
                     LLQERLPKTEASVLPEDLELEEARPRFGEGEDLMRVFKSPISRTPEIHLLSNGRYHVA
                     ISNAGGGYSRWKDLAITRWREDATCDYWGAFIYLRDVTTGELWSAAYQPTLRATKNYE
                     AIFTQARAEFRQRHGNLEIHTELSVSPEDDIELRRVTLTNHSSTERTIELTSYAEVVL
                     ATQAADETHPAFSNLFVQTEFVPGSSAILCTRRARMAEEKPPWLLHLLVGQGGTHGET
                     SCETDRARFIGRDGNLVNPAAMQKVAPLSNTAGSVLDPIISLRRTVTLQPDEIAILDF
                     VIGAAEDRETANALVEKYQHFRMADRAFDLAWTHSQVILRQLNATEAETQLYARLAGA
                     IIYADPARRATSGILLENRRGQSALWTYGISGDAPLVLLRVTNLEKIELVRQLIKAHS
                     YWRAKGLTVELVILNEDVSVYRQNLQDQITSLIAAGSEAQMLDKPGGIFVRRLEQIPN
                     DDRVLFQSAARIVLDDEQGSLLEQLDHRSVLEPPVPAFNASRAARVETPSPPPSRDLI
                     FRNGLGGFTPDGQEYVITLSPGQVTPAPWVNVLANPSFGTVISENGGAYTWFENAHEF
                     RLTPWFNDPVRDLTGEAFYIRDEETGQVWSPTPGPARGAAAYVIRHGFGYTVFEHTEH
                     GILSELWIYVAMDVPVKLAVFKLRNISGGPRRLSVTGYCEWVLGDLRHKTLLNVQTDV
                     DLKSGAL"
BASE COUNT          539 a          729 c          811 g          511 t
ORIGIN      
        1 agcgcgccac tcttgagatc gacatcggtc tggacgttga ggagcgtttt gtggcgcaaa
       61 tcgcccagca cccattcgca atagccggta acggaaaggc ggcgcggtcc gcctgagatg
      121 ttgcgaagtt tgaaaactgc gagcttcact ggcacgtcca tcgccacgta aatccacaac
      181 tcggaaagga tgccgtgctc ggtgtgctcg aaaacggtgt aaccaaatcc gtgccgaatc
      241 acataagcgg cggcgccgcg ggccggccct ggcgtgggcg accagacctg gccggtttct
      301 tcatcgcgga tgtaaaaggc ctcgccggta aggtcgcgca ccggatcgtt aaaccagggg
      361 gtgagccgga attcgtgcgc gttttcaaac caggtgtagg cgccgccgtt ttccgaaatg
      421 acggtgccga acgatggatt ggccagcaca tttacccagg gcgcgggcgt gacctggccg
      481 ggtgaaagcg tgatgacgta ttcctggcca tcgggagtga acccgccaag gccgttgcga
      541 aaaatgaggt cgcgcgacgg cggcggcgaa ggcgtctcga cccgggccgc gcggctggca
      601 ttgaatgcgg ggacgggcgg ctcgaggacg ctgcggtggt cgagttgttc caggagcgag
      661 ccttgctcgt catcgaggac aatgcgggcg gcagattgga aaagaacacg atcgtcattc
      721 gggatttgct cgagacggcg aacgaaaatg ccgccgggtt tatcgagcat ttgcgcttcg
      781 ctgccggctg cgatcagcga tgtaatttga tcctgcaaat tctggcgata aaccgaaacg
      841 tcttcattca aaatcaccag ctcgactgtc aaccctttcg cacgccagta ggaatgcgcc
      901 ttaatgagct ggcgcacgag ttcgattttt tccaggttgg tgacgcgcag cagaacgagc
      961 ggcgcgtcgc cggagatgcc gtaggtccaa agagcgcttt gtccgcggcg gttctccagc
     1021 agaattcctg aggtcgcgcg gcgggcggga tcggcataaa tgatcgcgcc agccagccgg
     1081 gcataaagtt gcgtttcggc ttcggtggcg ttgagctggc gcaggatcac ctggctgtgg
     1141 gtccaagcca gatcaaaggc gcgatcggcc atgcggaaat gttgatattt ttccactaac
     1201 gcgtttgcgg tttcgcggtc ttcggccgcg ccgatcacaa aatcgaggat ggcaatttca
     1261 tctggttgca gcgtgacggt gcggcggagc gagataatgg gatcgagcac ggaacccgcg
     1321 gtgttcgaaa gcggcgcaac cttttgcatc gccgccgggt tcaccagatt tccatcacga
     1381 ccgataaatc gggcacggtc ggtttcgcac gaggtttcgc cgtgagtgcc accctgccca
     1441 accagcaaat gcaggagcca gggtggtttt tcctcggcca tgcgcgctcg ccgggtgcag
     1501 agaatggcag acgagccggg aacaaattca gtctgcacaa acaaattgct aaaggccggg
     1561 tgcgtttcgt ccgcagcctg agtggcgagc acgacttcgg cgtaactcgt tagctcgatg
     1621 gtgcgctcgg tggaggaatg attggtcagg gtaacacgac gcagctcgat atcgtcttcg
     1681 ggcgaaacgc tcaactccgt atgaatctcg agattaccat ggcgctggcg gaattccgcc
     1741 cgggcctggg tgaaaatagc ttcgtaattt tttgtcgcgc gcagcgttgg ttgataagcg
     1801 gcggaccaga gctctcccgt cgtcacgtcc cgcagataga tgaacgcgcc ccagtaatca
     1861 caggtcgcat cctcgcgcca gcgggtgatg gcgagatctt tccagcgact atatccgccg
     1921 ccggcattac tgatagcgac gtgatagcgg ccattggaaa ggagatgaat ttccggcgtc
     1981 cgcgaaatcg ggctcttgaa gacccgcatg agatcttcgc cctcaccaaa ccgcggccgt
     2041 gcttcttcca gctcaaggtc ttcgggcaag acgctggctt ccgttttggg aagccgttcc
     2101 tgcaacaata aatcggccga cttcagcaat ggacgcgcca tgaagcgacg ttgcatcggc
     2161 aaatcgcgca gcaacgaaac gagggcgagc agactcattc cctggtgatg cgccatgtaa
     2221 gaacgaacgg tgacgctgct ctcatcgggc ggcagacgcg atggcgtata atcgactgcc
     2281 tcgtaaaatc cgtaagcgcc ttcgcgtcca tcgcgggcca ggcgctgcag attctcgctg
     2341 gcttcccgcg gcgctaccag caacgccagc gcggtggcat acggcgcgat gaccagatct
     2401 tcggccaggc cgcgtttcaa acccagcccc ggcacaccga aggcgcgata ttgatagatt
     2461 tggtgtgcat cgccctggtt aaagcccgat tccgaaacgc cccacgggac accgtgcgaa
     2521 ttgccgtact cgatttgcaa ttcaaccgcg gttttgcagg cgcgatcgag tagggtgccg
     2581 cggtagctcg
//
LOCUS       DASTPM010000308         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_789410, whole genome shotgun sequence.
ACCESSION   DASTPM010000308 DASTPM010000000
VERSION     DASTPM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789410"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..455
                     /locus_tag="VFR42_05415"
     CDS             258..455
                     /locus_tag="VFR42_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0368624.1"
                     /translation="MSESREVMNVRQASQYLGISPDTLYRYITEGEIPAFKLGNRWKL
                     RKTILDRWMERKMSQMHAKRR"
     gene            530..1579
                     /gene="pilM"
                     /locus_tag="VFR42_05420"
     CDS             530..1579
                     /gene="pilM"
                     /locus_tag="VFR42_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEU0368625.1"
                     /translation="MSLFGGKSKQLVGLDIGSSSIKSVELKSTKAGYELMSFGMESLA
                     PDTVVDGAIMDAPQVANAIGRIFDSSGVKTKNVATSVSGHSVIVKRVPLPLMNEEELY
                     DRIPSEASQHIPFDIADVNLSYQLLESMDAQMDVLLVAVKKDKILNHTNVLAQAGKTP
                     VVVDIDAFGLQNCFEVNYEPDAGQTVALLNIGASVMNINIVRGGIPLFTRDVSVGGNQ
                     YTDALQKELDLSFEDAERLKKGDTLPSVTDEQKQQILRSVSDILTLEIQKTFDFFRAT
                     ASGENIQRILVAGGTARVPGLVDLLREEFAMPVEELNPFRRVLINPGKHSEDQIREMA
                     PRLAIAVGLALRSFD"
     gene            1582..2205
                     /locus_tag="VFR42_05425"
     CDS             1582..2205
                     /locus_tag="VFR42_05425"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU0368626.1"
                     /translation="MIRINLLGQTRPKAARRPVDTGAALPAVFIGAGFLLGGLVLGYI
                     YYSWQSQLNAENNRIKQLQAQKTELQQIKQQVEQFEKQSKVLQVRVDTIEKLQRDRTG
                     GQELLDMVANTVSRTENLWLTNLTRKGNSLDILGSSASVNAVANFITAMKRSGYFDKI
                     EMTETKEDSNKNVQTFSFKLTAEINQSAPQAKPVSAPPQAAAPAKKS"
     gene            2215..2850
                     /gene="pilO"
                     /locus_tag="VFR42_05430"
     CDS             2215..2850
                     /gene="pilO"
                     /locus_tag="VFR42_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU0368627.1"
                     /translation="MANPLRDMSVIMQGLVAAAIAVVLVLLGIYLPFSPVAQEKDQYD
                     KAVQQRTQLTQEVTQLSVYKQRFGELKQQMDALNKQLDTLKTIVPEEKETDEFIRLLQ
                     GAATSSNVQIRHLTAKSVAPKDNNYYEMPFEVQADGPYFNVLDFFGRLSRLSRIINVG
                     DLQFSDVAKGGGYVMRPGTTVTGIFTATTYFTKPADAISTSSNAKPGAKKN"
     gene            2868..3485
                     /locus_tag="VFR42_05435"
     CDS             2868..3485
                     /locus_tag="VFR42_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368628.1"
                     /translation="MRLLITVATGMTLIAAPGMRAQNAQAVKNLANQAAAATGSKPAA
                     SGQAATKPAPAKSATVGQPAASKSAVGNSKSATPAAKTVSVKKPAESEQRIARRDPFE
                     SLVGRQQAQDALAKNLPPGKAGLQVGTLRLDGIVRSPNGMIAVVANPQQRTYFLREGD
                     KLYDGTVEKISMEGVTFHEEGKDAFGKPVERQVNKRLYSTAGEQQ"
     gene            3482..>4021
                     /locus_tag="VFR42_05440"
     CDS             3482..>4021
                     /locus_tag="VFR42_05440"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEU0368629.1"
                     /translation="MKTNWPALRNGCLILASLACSAAPMLAQSHEAAAVAPAAAVISS
                     VAVTQASARSSVRVEGEGELYARATRMQNPDRLVLDFTSTRMAVQKTVIPGVSAPVRG
                     VRLGQYRPDVARVVIDLTAATPYQIAHEGSALVISFDTQDSRVISNAPTLTAATEAAK
                     SDFRYADAAPRGTNHRGAAL"
BASE COUNT          931 a         1172 c         1194 g          724 t
ORIGIN      
        1 actgatgctc ctatacctgc atatatagag tacagtgccc atgataaccc tagaattccc
       61 taagattccc cctattctta ggacaactgc cggagttaag cgcttgacca ctatcgtttc
      121 cggatgggat aacgctgaca ccggacgcgc tcccgatttc tcggtgcaag tgcgggagcg
      181 cagtcagcag ttcagttcgg gcgtgttcca gtttcaagcg gtaacgaaaa tgacggtcga
      241 tggagttgtg ttccaccgtg agcgaatctc gcgaagtgat gaatgtgcgg caagcgtcgc
      301 agtatctggg catcagcccg gacacgctgt accggtacat caccgaaggc gaaattccgg
      361 cgttcaagct gggcaaccgc tggaaactcc gtaagacgat tttggaccgc tggatggaac
      421 gcaagatgag ccagatgcac gccaagcgtc gttaagagac tttgttgaag taaaaagggc
      481 tcgcggattt cgagcagttt gtagcaggaa agaataaagg ggctgcaaga tgtcgttatt
      541 cggcggaaag agcaagcagc tagtggggtt ggacatcggt tcgagctcga tcaaatcggt
      601 ggagctcaag tccacgaaag cgggctacga actgatgagc ttcggcatgg agtcgctggc
      661 gccggacacg gtggtggacg gagccatcat ggacgcgccg caggtggcca atgccatcgg
      721 gcgcatcttc gattcttcgg gtgtgaaaac caagaacgtc gcgacttcgg tttccggtca
      781 ctcggtgatc gtgaagcggg tgccgctgcc cctgatgaac gaagaagagc tgtacgatcg
      841 cattccttcc gaagccagcc agcacattcc tttcgacatt gccgacgtga atctcagcta
      901 ccaactgctg gagtccatgg acgcgcagat ggacgtgctc cttgtcgccg tgaagaaaga
      961 caaaattttg aaccacacca acgtgctggc acaggccggc aagacgccgg tggtggtgga
     1021 tatcgacgcg ttcggcttgc agaactgctt tgaagtgaac tacgaacccg acgccggaca
     1081 gacggtggcg ctgctcaaca tcggcgccag cgtgatgaac atcaacatcg tgcgtggcgg
     1141 cattccgcta tttacgcgcg acgtcagcgt gggcggcaac cagtacaccg acgcgctgca
     1201 aaaggaattg gacctgagct tcgaggacgc cgagcggttg aaaaagggcg acacgctgcc
     1261 cagcgtcacc gacgaacaga agcagcagat cctgcgcagc gtcagcgaca tcctgacact
     1321 ggaaattcag aaaactttcg acttcttccg cgccaccgcc agcggtgaga acatccagcg
     1381 catattggtg gccggcggaa cggctcgcgt gcccggtctg gtggacctgc tgcgcgaaga
     1441 attcgccatg ccggtggaag agctgaatcc tttccggcgc gtgctgatca atcccggcaa
     1501 gcacagcgaa gatcagattc gagagatggc gccgcggctg gcgattgccg tgggcctggc
     1561 gttgaggagc tttgactagc catgatccgc ataaaccttc ttggacaaac gcggcctaag
     1621 gcagcaaggc gtcccgtgga taccggggcg gcgctgccgg cggtcttcat cggagcggga
     1681 ttcctgctgg gcggcctggt gctcggctac atctactatt cgtggcagag ccaactgaac
     1741 gccgaaaata atcgcatcaa gcaactgcag gcgcaaaaaa cggaactcca gcaaatcaag
     1801 cagcaggtcg aacaatttga aaagcagagc aaggtcctgc aggtccgcgt ggacaccatt
     1861 gaaaaactgc agcgcgaccg caccggcggc caggaactgc tggacatggt ggctaacacc
     1921 gtgtcgcgca cggaaaatct ttggctgacg aacctcaccc gcaagggcaa ttcgctggac
     1981 atcctcggct cctcggcatc ggtgaatgcg gtggcaaact tcatcaccgc catgaagcgc
     2041 tctggctatt tcgacaagat cgaaatgacg gagaccaagg aagacagcaa caaaaacgtg
     2101 caaacctttt cgttcaagct cacggcggaa atcaatcaat ccgcgccgca ggctaagcct
     2161 gttagcgcac ccccgcaggc cgccgcgccc gcgaagaaga gttaggagag cgacatggcc
     2221 aatccattgc gcgacatgtc ggtgattatg cagggcctgg tggccgcggc gattgccgtg
     2281 gtgctggtgc tgctgggaat ttacctgccg ttttcgccgg tggcgcagga aaaagatcaa
     2341 tacgacaaag ccgtgcagca gcgcacgcag ctcacccagg aagtgaccca gctttcggtg
     2401 tacaaacagc gcttcggcga actgaaacag cagatggacg ccttgaataa gcagctggac
     2461 acgctgaaga ccatcgttcc cgaagaaaag gaaaccgacg aatttatccg cttgctgcag
     2521 ggcgcggcca cttcgtccaa cgtccagatt cgccacctga cggccaagtc ggtggcgccc
     2581 aaggacaaca actactacga gatgccgttc gaagttcagg ccgatggtcc gtacttcaac
     2641 gtgctggact tctttgggag actcagccgc ctctcgcgca tcatcaacgt tggtgacctg
     2701 caattttcgg acgtcgccaa aggcggcggc tatgtgatgc gtccggggac caccgtgacg
     2761 ggaatcttca ccgcgacaac ctatttcacc aaaccggccg acgcaatttc gacttccagt
     2821 aacgccaagc cgggagccaa gaagaattga gcgggaataa gcaaactatg cgccttctga
     2881 ttacagttgc cacaggaatg acgctgattg ccgcgcccgg aatgcgcgcg caaaatgcgc
     2941 aagcggtcaa gaatctcgcc aaccaggcag cggcggcgac cggttcgaag ccggctgctt
     3001 cgggccaggc cgccaccaaa cccgcacccg ccaagtcggc cacggtcggc caacccgcag
     3061 cctccaaatc ggcggttggc aattccaagt ccgccacgcc agccgctaaa accgtttccg
     3121 tgaagaagcc ggcggaaagc gagcagagaa tcgcccgccg cgatccgttc gaatcactgg
     3181 ttggccgcca gcaggcgcag gacgccctgg ccaagaacct tccgccgggc aaagccgggt
     3241 tgcaagtggg cacgcttcga ttggacggca tcgtccgctc gccgaacgga atgattgccg
     3301 tagtagccaa tcctcagcag cgcacctact tcttgcgcga gggcgacaag ctctacgatg
     3361 gcaccgtgga aaagatttct atggaaggcg tcaccttcca tgaagagggc aaggacgcgt
     3421 tcggcaaacc tgttgagcgc caagtgaaca aaagacttta ctcgaccgca ggagaacagc
     3481 aatgaagacg aactggccgg ccttacggaa tggctgcctt atccttgcaa gcctggcgtg
     3541 cagtgcggcg cccatgctgg cacagtccca tgaagcggct gccgttgcgc ctgcagcggc
     3601 ggtaatctcc agcgtcgcgg tcacccaggc ctccgcgcgg tccagcgtgc gcgtcgaggg
     3661 cgaaggcgaa ctctatgccc gtgcgacgcg gatgcagaat cccgaccgct tggtgctgga
     3721 tttcacctct acccgcatgg ccgtgcaaaa aacggtcatc cccggcgttt cggcgccggt
     3781 gcgcggcgtg cggctcgggc aatatcggcc cgatgtggcc cgcgtggtga ttgatctgac
     3841 tgcggctact ccgtatcaga tcgcccacga aggcagcgcc ctggtaattt ccttcgatac
     3901 ccaggattcg cgggtaattt ctaacgcccc cacgctgacc gctgccacgg aagccgccaa
     3961 gtcggacttc cgctatgccg atgcggcccc gcgcggaaca aatcatcgcg gagctgcgtt
     4021 g
//
LOCUS       DASTPM010000309         7323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1048878, whole genome shotgun sequence.
ACCESSION   DASTPM010000309 DASTPM010000000
VERSION     DASTPM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048878"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /gene="recG"
                     /locus_tag="VFR42_05445"
     CDS             complement(<1..2110)
                     /gene="recG"
                     /locus_tag="VFR42_05445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU0368630.1"
                     /translation="MPEGLQTDVRMLKGVGPQRAELLAKRGIYTLEDLLGYLPFRYED
                     RIHFSLIKDIRPGNVYTLRVRVASGSAVRGMRGRDAIYHLLVKDETGFSLACKFFHGG
                     YLEGRLKSGQLLVLHGKVEVDRLRPARMEMVNPQFELLSDDPAGQNDGQSDARPADST
                     EVGRIVPIYEAIGTFSTRAIRRATYAALQRLDPNFPDLLPASIRRRMNFPLRRDAIIQ
                     THFPPPDSSLDALNQFRAPAQQRLIFEEFFFYQLSMALNRKLARKDNAIAFRVREDRV
                     RDALKRMLPFKPTAAQKRVLAEIAADLEKPAPMNRLLQGDVGSGKTIVALQAAVIAIE
                     NGCQAALMAPTEILAVQHYFAARRLLEKAGYKVELLISGLKPSEKSAARDRIANGQAR
                     LVVGTHALIEDDVLFSRLGFVAIDEQHRFGVLQRKELMDKAATHGHAPHVLVLTATPI
                     PRTLSLTLYGDLDVSVLDELPPGRTPIETHMTTESHLAGVWESLRREVAAGRQGYIVY
                     PVIEESKLELKAAIEEYERLSIQVFPKLKLGLLHGRLSSEEKDAVMQAFRNNETQILV
                     ATTVIEVGVDVPNATVMVVEHAERFGLSQLHQLRGRIGRGVHKSHCVLIAPGRMTEEA
                     RARLDTMVRTNNGFEIAEADLQIRGPGEFFGTRQSGEMGFHVANPLRDKEFLETARKE
                     AFSLADDAGRRDELKQTLRAL"
     gene            complement(2661..3515)
                     /locus_tag="VFR42_05450"
     CDS             complement(2661..3515)
                     /locus_tag="VFR42_05450"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU0368631.1"
                     /translation="MNPILVIGATGMVGRQVVSQLIAAGSPVRAMARKPEAANLPPQV
                     QVVRGDLTVPETLDACLDGADAVFLVWTAPPSAVAPALQRITQRARRIVYLSAPLKTP
                     HPLFQQPNLLRPMFEQIERTIVASGVPWTFLRPGMFAPNALLWWAPQIRAGNLVRWPY
                     LAVPTAPIDERDIATVAVRALCEEGHAGAEYVLTGSQSLTHSEQISTIAGVLGRELRV
                     EEISPDEARRELLTIGPLPAVNMLLDAWSAALGHPAHMTSTFTQITGAPAKSFRTWAT
                     DNASAFRA"
     gene            3630..4088
                     /locus_tag="VFR42_05455"
     CDS             3630..4088
                     /locus_tag="VFR42_05455"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEU0368632.1"
                     /translation="MRSSDSRPLRLIALFKLLKALLLVIVGVSGLRLIHTDITGLATE
                     WVLRLGLDPGSRQVGRFLVKAAALTPSRLRELGVGSLVYAGLFLTEGLGLWRLKRWAE
                     WLTVLITSSLVPVEAYEIYQHPTVVRVLLVLVNLGVVAYLVYRIRSDRAK"
     gene            4199..>7323
                     /locus_tag="VFR42_05460"
     CDS             4199..>7323
                     /locus_tag="VFR42_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0368633.1"
                     /translation="MAKVQLSGRDKRALILWVLAGVVGLWYAQRHFFEAFPEASVNFK
                     VTRGEALERARNFVESLGHSTAGYRSVIVFGVDDNAKTYLEREVGLKEANRLMASEVN
                     VWHWQVRFFKPEQEEEFLVGVSPEGKITGYVHKIPEAQAGAEPTRDAAQQTAQNFLTA
                     KLGKAATDWDFLPEQANSAKKPNRLDWSFTWEKHGFKAKDAPERLTIALHGAEVGSAH
                     EAPKVPEQWERDYRHLRSTNEFYNTIAIIPYLLLLGGVLWYGIQLTRRGQTNWRLALQ
                     LGVMVAVLLTAMQLNRWPLDVFGYNTDSSYGSFTIQQILGALAFGIFSALTVSLVLPG
                     GEPFYREAKPHFLRLKKALTWRGLRTREFFSSVVVGLSLAGAHMGFLVAFYLIANHFG
                     AWSPQEVNYEDSVSTAIPWIGGIAIGLLAATSEEFLFRLFAIPFLQKFTKSNVLAVIL
                     PAFSWGFLHTAYPNEPPYIRGLEVGLIGIVAGVVMLRWGILATLVWHYTVDAALVGLL
                     LIRSTSLYFRISGIVVGLAILIPFGYAVCARVKRGTFEEDEDLLNRTPDPEDAVTAKE
                     AEPARQLGHAPTGRLSKGVLAFLAVCVVLGAAAAVKLKPVHLGDYLKLSINTKEAGAK
                     AQDILKQRGVEPQSYKSVTTFVDVTDPTASEYLRQKIGVPALNNIYEKQVPGGLWDTR
                     FFRDGQAEEYSVILRPDGSLHSVHHDLAEAAKGASLSKEEAIAKATAYLQNEKRIDLA
                     NWNLVDSSSEKRPNRIDHTLIWQAKQPLDSESATANADPATHAFQRMQVIVAGDEPTS
                     FRTFIKIPEEWRRKQESQSIWRTLHTVLVMCLAVGLSIAALIFFLREIKSDLMKQIPW
                     RRLTFWGLFALAAYVAIGVFGDRIPAFLSQYKTANPLKFMYGGLAIGFTIGAFFYLGL
                     LVILFAMGWFFLKQAYGEVAWPGWRGMTREYYRDALFIGLGGTAALIAVSRATEFVFR
                     RWPTPHRSFPASFGLDFDSYVPGISIGASAALRGLLVAGLIAAVGGFVLAHCKSPMLR
                     ALLFVTTSVALV"
BASE COUNT         1434 a         1772 c         2593 g         1524 t
ORIGIN      
        1 gcagtgcccg cagagtctgc ttgagttcgt cgcgacgtcc ggcatcatct gccagagaaa
       61 aagcttcttt gcgcgcggtt tccagaaatt ccttgtcgcg taacggattg gcgacgtgga
      121 agcccatttc gccggactgg cgagtgccaa aaaattcacc cggtccacgg atttgcagat
      181 cggcttcggc aatttcgaaa ccgttgttgg tgcggaccat ggtgtcgagg cgggcgcggg
      241 cttcctccgt catacggcca ggagcaatca agacgcagtg ggacttgtgc acgccgcgac
      301 ctatgcgtcc gcgcaattgg tgcaactgcg aaaggccgaa acgttcggcg tgttcgacaa
      361 ccatgacggt ggcgttggga acgtcgactc cgacttcgat gacggtggtg gcgacgagga
      421 tttgcgtttc gttattgcgg aaggcctgca tgacggcatc tttttcttcg ctggagaggc
      481 ggccgtgcag caggccgagc ttgagtttcg ggaagacctg tatcgaaagg cgttcgtact
      541 cttcgatggc agctttgagt tcgagtttcg attcttcgat aaccggatag acgatatagc
      601 cctggcggcc ggcggcaact tcgcggcgca gcgattccca cacgccggcc agatgcgatt
      661 cggtggtcat gtgggtttcg atgggagtgc ggccgggggg aagctcgtcg agaacagaaa
      721 cgtcgagatc gccgtagagc gtgagcgaaa gcgtgcgtgg gatgggcgtg gcggtgagga
      781 cgagaacgtg aggcgcgtga ccgtgggtgg cagctttgtc catgagctct ttgcgctgga
      841 gaacgccgaa gcggtgctgc tcgtcaatgg cgacgaagcc gaggcgggag aagagaacgt
      901 catcctcgat gagggcgtga gtgccaacga cgagccgcgc ctggccattg gcgatgcgat
      961 cgcgtgcggc ggatttttca gatggcttta gaccgctgat gaggagctcg actttgtagc
     1021 cggctttttc gaggaggcgg cgtgcggcaa aataatgttg gacggcgaga atttcggtcg
     1081 gcgccatgag agcagcctgg cagccgtttt cgatggcgat gaccgcggcc tgcaacgcga
     1141 caatggtttt gccgctgccg acgtcgcctt gcaggaggcg gttcatgggt gcgggctttt
     1201 ccagatcagc ggcaatttcc gcgaggacgc gtttttgcgc ggcggtgggc ttgaacggga
     1261 gcatgcgctt gagggcgtcg cggacgcggt cctcgcgcac gcggaaagcg atggcgttgt
     1321 ccttgcgggc gagcttgcga ttgagcgcca tgctcaactg gtagaagaaa aattcctcga
     1381 agatgaggcg ctgctgcgcg ggagcacgga actggttgag cgcatcgagc gaagaatccg
     1441 gcggagggaa atgagtttga atgatggcgt cgcggcgcaa cggaaaattc atacggcggc
     1501 gaatggacgc gggaagcaga tccgggaagt taggatcgag acgctgcagc gcggcatagg
     1561 tagcacgtcg aatggcgcgc gtgctgaagg tgccgatggc ttcatagatc ggaacgatgc
     1621 ggccgacttc ggtggagtcg gcgggtcgag cgtcgctctg gccgtcgttc tgcccggcgg
     1681 ggtcgtcgct gagtagttcg aactggggat taaccatttc catgcgggcg gggcggaggc
     1741 gatcgacttc gactttgccg tgaaggacga gcaactggcc cgacttgagg cggccttcga
     1801 gatagccgcc atgaaaaaac ttgcaggcga gggagaaacc ggtttcgtcc tttaccagca
     1861 gatgatagat ggcgtcgcga ccgcgcatgc cacgcacggc agaaccgctg gcgacgcgca
     1921 cgcgcaacgt atagacgttg cccgggcgta tatccttgat cagggaaaag tggatgcggt
     1981 cttcgtagcg gaacggaaga tagccgagca ggtcttcgag cgtgtagatg ccgcgcttgg
     2041 cgaggagttc ggcgcgctgc gggccgactc ctttgagcat gcgcacgtcg gtttgcaggc
     2101 cttcgggcat gcttggagtt atagccaaaa agggtgggca gcacaaaggc gggcagcggg
     2161 actctggtta gagcgtcaat aaggtcgccg cgataaattc taaatcggag atgagcgaag
     2221 gcctttccga ctgcttccgc ttaactttcc gtccgaattg ggcgcggcag cttttcgaat
     2281 aggcgaagaa gtggcatggc cgccgacgct cgcggtggcg gcagttgagt ggttcagtag
     2341 gaatggatac gccgtgcttg aaacggagct ttggctttgc cggaccggga aattcaaaag
     2401 cgtaccgctc ggactcagcg gaatcgcggc aataccgtaa accggggaac cgaagaatcg
     2461 tggagttcat ttagtgcacg cgctgaagca gaaccccgcg acactctgga aacgttcaaa
     2521 ccctccatat tttcgagcga ggtcagttac gttcaacgtg gtttggcagc gaagccgagt
     2581 tccagaatct tgcggacgag ctcttggtaa cctaatcggt cggactgagg aacacgtgca
     2641 ttttgggtgg gcgcatcggc ctaggcgcga aaggcggagg cattgtcggt tgcccaggtg
     2701 cggaaggatt ttgcgggggc gccagtgatt tgtgtaaacg tggaagtcat gtgggcgggg
     2761 tggccgaggg cggcggacca ggcgtcgagg agcatgttga cggcggggag gggaccgatg
     2821 gtgaggagtt cgcggcgcgc ttcatcgggg gagatttctt cgacacgcag ctcgcggccg
     2881 agcacgccgg cgatggtaga gatttgttcg gagtgagtga gcgattgtga accggtgaga
     2941 acatattcgg cccccgcgtg gccttcttcg cacaaggcgc ggacagcgac tgtggcgatg
     3001 tcgcgctcat cgatcggcgc ggtgggaacg gcgagatagg gccagcgaac taggttgccc
     3061 gcgcgaatct gcggcgccca ccaaagcaaa gcattggggg cgaacatgcc ggggcggaga
     3121 aaagtccacg ggacgccgga ggctacgatg gtgcgttcga tttgttcgaa catcggccgg
     3181 agcagattgg gctgctgaaa gagcgggtgc ggcgttttga gcggagcgga aaggtagacg
     3241 atgcggcggg cgcgctgggt gatgcgctgc aaagccggag ctaccgcgga aggtggggcg
     3301 gtccacacga gaaagacggc gtcggcgcca tcgaggcatg catcgagagt ttcgggaacg
     3361 gtgagatcac cgcgaacgac ctggacctgg ggcggcaaat tggcggcttc tggtttgcgg
     3421 gccatggcgc gcaccggcga gccggcagcg attagttgag agacgacctg gcggccgacc
     3481 atgccggtgg cgccgattac gagaatggga ttcatgggac tccattctaa cctgcggact
     3541 taaattccaa cggatccaaa gcaaagagcg gtgctcctgg aattgaggtt gaccagggaa
     3601 gagccagcta tcggctatga tgaaacggga tgaggtcctc ggacagcagg ccgctccggc
     3661 tgatcgcgct tttcaagctg ttgaaggcgc tccttctggt catcgttgga gtgagcgggc
     3721 tgagattgat ccacaccgac atcaccggcc tggcgaccga gtgggtgctt cggctcggct
     3781 tggatccagg gagccggcag gtgggccgat ttttggtcaa ggcggcagcg cttacgccga
     3841 gccggttgag ggaactggga gtcgggagcc tggtgtatgc cggactgttt ctgaccgagg
     3901 gactcggact gtggcggttg aaacggtggg ctgagtggct cacggtgctc atcaccagtt
     3961 cactggttcc agtggaagct tacgaaatct accagcatcc gacggtggta cgagtcttgc
     4021 tggtgctcgt caatctcggg gtagtggcct acctcgtgta ccggattcgt agcgaccgcg
     4081 cgaagtagaa cgcggaaacg ggagggaggt tacttgcgcg gtttggcggc cttatgaaag
     4141 agtgggtggt tgagcggttg tgcgaattcc gttggtgagt gcgggaagcg gaggcgtggt
     4201 ggcaaaagtc cagctaagcg ggcgagacaa gcgggcgctg atcttgtggg tgctggcggg
     4261 cgtggtgggg ctgtggtacg cgcaacggca tttttttgag gcgtttccgg aagcgtcggt
     4321 gaacttcaag gtgacgcgtg gagaagcgct ggaacgcgcg cgcaattttg tagagtcgct
     4381 ggggcacagc acggcaggct accgctcggt gatcgtgttt ggcgtggacg acaatgcgaa
     4441 gacgtacctg gagcgggaag tcggattgaa agaagccaat cggctgatgg cttcggaagt
     4501 gaatgtgtgg cactggcagg tgcgtttttt caagccggag caggaagagg agttcttggt
     4561 tggcgtgagc ccggaaggaa agatcaccgg gtacgtgcac aagattcccg aagcgcaagc
     4621 gggcgcggaa cctacgcgcg atgcagcgca gcagacggcg cagaattttc ttacggcaaa
     4681 gcttggaaag gcggcgacgg attgggattt tttgccggaa caggcgaact cggcgaaaaa
     4741 gccgaaccgg ctggactggt cgttcacttg ggaaaagcac ggatttaaag cgaaggatgc
     4801 gccggagcgg ttgacgattg cgctgcatgg cgcggaggtt ggatcggcgc atgaggcgcc
     4861 gaaagtgccg gagcagtggg agcgggatta ccggcacctg cgctcgacca atgagtttta
     4921 caacaccatt gcgattattc cgtacttgct gctgctgggc ggagtgctgt ggtatggcat
     4981 tcagctgacg cggcgtgggc aaacgaattg gcggctggcg ctgcagcttg gcgtgatggt
     5041 ggcggtgctg ctgacggcaa tgcagctgaa tcgctggccg ctagacgtgt ttgggtacaa
     5101 cacggattcg tcgtacggca gcttcacgat tcagcagatt cttggagcgc tggcatttgg
     5161 aattttttcg gcgctgacgg tgagcctggt tttgcccggc ggcgagccgt tttatcgcga
     5221 agcgaagccg cactttttgc gtttgaaaaa agcgctgacg tggcgcgggt tgcgaaccag
     5281 ggaatttttc tcgtccgtgg tggtgggact gtcgctcgca ggcgcgcaca tgggatttct
     5341 ggtggcgttc tatctgatcg ccaaccattt cggtgcgtgg tcgccgcagg aagtgaatta
     5401 cgaagattcg gtgagtacag cgattccgtg gattggcggg attgcgatcg ggctgctggc
     5461 ggcgaccagc gaagaattct tgttccggct gttcgctatt ccctttttgc agaaatttac
     5521 gaagtcgaat gtgctggcgg tgattcttcc cgccttttcg tggggatttc tgcacacggc
     5581 gtatcccaat gagccgccgt acattcgcgg gctggaagtt gggctgatcg gaatcgtggc
     5641 gggtgtggtg atgctgcgct ggggaattct ggcgacgctg gtgtggcact acacggtgga
     5701 cgcggcgcta gtggggctgt tgctgatacg ttcgacgagc ttgtattttc ggatttcggg
     5761 catcgtggtg ggcctggcga tcctgattcc gttcggctac gcagtctgtg cgcgcgtgaa
     5821 gcgcgggacg tttgaagagg acgaagattt gctgaaccgc acgcccgacc cggaagatgc
     5881 tgtgaccgcg aaggaagcgg aaccagcgcg ccagttgggg catgcaccga cgggacggtt
     5941 gtccaagggg gtgctagcgt ttctcgcagt gtgcgtggtt ctgggcgccg cggctgcggt
     6001 aaagctgaaa cccgtgcatc tgggcgatta cttgaaactt tccatcaaca ccaaggaagc
     6061 gggggcaaag gcgcaagaca tcttgaaaca gaggggcgtt gagccgcaga gctacaaatc
     6121 ggtgacaacg tttgtggatg tgacggaccc gacggcaagc gaatacctgc ggcagaaaat
     6181 cggcgtgccg gcgttgaaca acatttatga gaagcaggtg ccgggcggtt tgtgggacac
     6241 gcgatttttc cgggatggcc aggcggaaga gtattcggtg attttacggc cggatggttc
     6301 gctgcatagc gtgcatcacg acttggccga ggcggcgaaa ggcgcttcgc tttccaagga
     6361 ggaagcgatc gcgaaagcga cggcgtattt gcagaatgaa aagaggatcg atcttgcgaa
     6421 ctggaacctg gtggattcga gttcggagaa gcgaccgaat cgaattgatc acacgctgat
     6481 ctggcaggcg aagcagccgc tggattcgga gagcgcgaca gcgaacgccg atccggcgac
     6541 gcatgcgttt cagcgaatgc aagtgattgt ggcgggcgac gaaccgacca gtttccgcac
     6601 atttatcaag attcccgagg aatggcggcg caagcaggaa tcgcagtcca tctggcggac
     6661 gctgcacaca gtgctggtga tgtgcctggc ggtgggtctg agtattgcgg cactgatttt
     6721 ctttttacgg gaaatcaaat cggatttgat gaagcagatt ccctggcgcc gactcacgtt
     6781 ctggggtttg tttgcgctgg cggcctatgt ggcgattgga gtattcggcg accgcattcc
     6841 ggcgttcttg agccagtaca agacggcgaa tccgttgaaa ttcatgtacg gcgggctggc
     6901 gattggattc accatcggcg cgtttttcta cttaggactg ttggtgattt tgttcgcgat
     6961 gggctggttc ttcctgaagc aagcgtatgg agaagtagcg tggccgggct ggcgcggcat
     7021 gacgcgagag tactatcgcg acgcactgtt cattggtttg ggcggaacag cggctctcat
     7081 cgcggtgagt cgcgcgaccg aatttgtctt tcgccgctgg cctactccgc accgctcgtt
     7141 tcccgccagc ttcgggctgg actttgattc ttacgtaccg ggcatttcca ttggcgcatc
     7201 ggcagctttg cgcggtttgc tggtcgcggg actaatcgcc gcagtgggcg gatttgtgct
     7261 ggcgcactgc aagtcgccaa tgctccgcgc gctgctgttt gtgaccactt cggtggcgct
     7321 ggt
//
LOCUS       DASTPM010000310         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_183971, whole genome shotgun sequence.
ACCESSION   DASTPM010000310 DASTPM010000000
VERSION     DASTPM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183971"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..950)
                     /locus_tag="VFR42_05465"
     CDS             complement(102..950)
                     /locus_tag="VFR42_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368634.1"
                     /translation="MADTPLQVTGNPQAPQRNDPASIYEILIGSAKEAVLRVQEYSYL
                     AAQSVTNIFSRPHYVQDTLDQMDSIGVGSLPIVLLTGFFIGAVMVLQTASQFVRFGQT
                     SLTGDAVALALVRELGPTLTSLLVTGRCASGIASELGSMLVTEQVDAMRAMGTDPSRK
                     LVTPRVVATVLVVPLLTTMSDFIGLVGGCVAAVFSLRLGVVQFWTRAIDALEFSDIMQ
                     GMMKSIVFAFILSTVGCYKGLTVRGGTQGVGRSTTQAVVISSVMIIVADTFLTKLALY
                     LADKLF"
     gene            complement(1128..1916)
                     /locus_tag="VFR42_05470"
     CDS             complement(1128..1916)
                     /locus_tag="VFR42_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0368635.1"
                     /translation="MSQIGKFTQPDASPAYFIEFLEFLDRREDIRHVRAQAAKGLNLA
                     AGHKVLDLGCGIGGATFPLAEITGPTGLAAGVDISAAMVEAASRRVNGQAGIEFQRGE
                     ACSIPYPDKFFDAARSERVFLYLPDRLAAIQEIKRVVKPDGRVCLIDVDLDCTAVYSK
                     NPALARKLTSIVAASMPNANSARDLPALARQAGLKDVKTEVSAISTPYEFFLRVMAGT
                     LISAAEQGIVSRAEVDEWMQEQALLKDSGDFFQLWFMVVVCGTV"
     gene            complement(2066..2992)
                     /locus_tag="VFR42_05475"
     CDS             complement(2066..2992)
                     /locus_tag="VFR42_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0368636.1"
                     /translation="MRFMGLAVVFALLFPVTFLGQEPDPSVMRVAVNLVMVDATVKTK
                     AGQILGDLKKEDFELREDGAAQKVEIFSRDELPLNVALVLDLSDSIEPFLGPLRDAAN
                     TALGALKPEDEVALFTFSTEAELRVPFTRDKKQLAEQINAFKVGGATNINDGIFVPAK
                     YLLDAPNRGRRVIILISDDVATYSGGEGTRDIITELIASDAVLYNLKIPGYNPPGTML
                     HAAMEKGLVNLRAVMEQTGGEIFNVQDVSHLDAAFRALIERIKTRYTLGYYTKASAAE
                     GKPHKLDVRLAASHGKKGKDYVVLSKNGFYVH"
BASE COUNT          657 a         1068 c          688 g          624 t
ORIGIN      
        1 cggatacacc tcaaacgaca gcccgtgcac gatgtcgccc tcttcaaatc ccaggcgtac
       61 atcgcggaag gcaatcgtcg cttccgattt ctgttgcgtg ttcaaaagag cttgtccgcc
      121 aggtacaacg ccagtttggt caggaacgtg tccgccacaa tgatcatcac cgacgaaatc
      181 accaccgcct gcgtggtcga acgtcccact ccctgcgttc cgccgcgcac cgtcaaacct
      241 ttgtaacagc ccaccgtgga caaaataaac gcgaacacga tggacttcat catcccctgc
      301 atgatgtcgg aaaattccag cgcatcaatc gcccgcgtcc agaactgcac cacacccagc
      361 cgcaacgaaa ataccgccgc cacgcatccg cccaccaatc caatgaaatc cgacatggtc
      421 gtcagcagcg gcaccaccaa aactgtcgcc accacgcgcg gcgttaccag cttgcggctc
      481 gggtccgtgc ccatggcccg catggcgtcg acttgctcgg tcaccagcat cgagcccagc
      541 tccgacgcaa tccccgacgc gcaccgcccg gtcaccagca agctggtcag cgttggcccc
      601 agttcgcgca ccagtgccag cgccaccgcg tcgccagtaa gcgaagtctg cccaaagcgc
      661 acaaactgtg acgccgtctg cagcaccatc accgcaccaa taaaaaaccc cgtcagcagc
      721 acaatcggca gcgaacccac accgatgctg tccatctggt ccagcgtgtc ctgcacgtaa
      781 tgcggccgcg agaatatatt ggttacggat tgggctgcca ggtacgagta ttcctgcaca
      841 cgcagcaccg cttccttggc gctgcctatc aatatttcgt aaatcgaggc gggatcgttc
      901 ctctgcggtg cttgcggatt gccggtgacc tgaagaggcg tgtctgccac gtgttcctgt
      961 tccgcgacga tctcccggcg cacctggttc cttgcttgga aattacgccg gccacaacta
     1021 ggtcacagaa aagtacttcc ccgaaagcct aaccgcgcct tcgcattgcg tcaagaaaag
     1081 ttgtgttttt gcacgtcgcg ccggccgaaa ttttctgtca ccagccctta caccgttccg
     1141 cagacgacta ccataaacca caactggaaa aagtctccgc tgtccttcag cagcgcctgc
     1201 tcttgcatcc attcgtccac ttccgcgcgt gaaacaatgc cttgctctgc cgcacttatc
     1261 agagttcccg ccatcacccg cagaaaaaac tcatacggcg tggagatcgc acttacctcg
     1321 gtcttcacgt ccttcaaacc cgcttgtcgc gccaacgccg gtaaatccct agccgaattc
     1381 gcatttggca tactagccgc cacaatcgac gtcagcttgc gtgcaagcgc agggtttttc
     1441 gaatacaccg ccgtgcaatc taggtccacg tcgatcaggc atactcgccc gtctggcttt
     1501 accactcgct tgatttcttg aatcgcggcc agccggtcgg gcaggtacaa aaacactcgc
     1561 tcgctgcgcg ccgcgtcgaa aaacttgtcc ggatacggaa tcgagcaagc ctctccacgc
     1621 tgaaattcaa ttccggcctg tccattcaca cgacggcttg ctgcttcaac catcgctgcg
     1681 ctgatatcca ctccagctgc caaaccggtc ggcccggtaa tttccgccag cgggaacgtc
     1741 gcgccgccaa tcccacagcc caaatcgagt accttgtgcc cggcggccag gttcaagccc
     1801 ttcgccgctt gcgctcgaac atgccgaatg tcctcgcgcc tgtcgaggaa ctccagaaat
     1861 tcaatgaaat atgccggcga cgcatccggc tgcgtgaact tcccaatttg tgacatatcg
     1921 ctttctaagc tccagttggc gagacgatac cacgcccctt acccctcaac aaaatatccg
     1981 cgccgttaca cgctcgaaac caacgggcat ccagccgcag gcccgccctc ggtcttcgag
     2041 tttcgaattt cgctctcctg ccccttcagt gcacataaaa tccgttcttc gacagcacca
     2101 cataatcctt gcccttcttc ccatgtgaag ccgccagccg cacatccagc ttatgcggct
     2161 tcccttccgc cgcgctggcc ttggtgtagt accccagcgt gtagcgcgtc ttgatccgct
     2221 cgatcagcgc acgaaacgcc gcgtccaggt gcgatacatc ctgcacatta aaaatttctc
     2281 cgccggtctg ctccatcacc gcgcgcagat tcaccaaccc cttctccatc gccgcgtgca
     2341 gcatcgtccc cggcgggtta tagcctggaa ttttcaggtt gtacaaaacc gcatccgacg
     2401 caatcagttc cgtaatgatg tcgcgcgttc cctctccgcc cgaatatgtc gccacatcat
     2461 cgctgatcag gatgatcacc cgccgaccgc gattcggcgc atccagcaaa tatttcgccg
     2521 gcacaaagat tccatcattg atgttcgtcg cgccgcccac tttgaacgcg ttgatctgct
     2581 cggccagctg tttcttgtcc cgcgtgaacg gcacacgcag ctccgcttcc gtcgaaaacg
     2641 taaacagcgc tacttcatct tccggtttca gcgcgcccag cgcggtgttc gccgcgtccc
     2701 gcagcggccc cagaaacggt tcgatcgaat cgctcaaatc cagcaccagc gccacattca
     2761 gtggcaactc gtcccggctg aaaatctcca ccttctgagc tgccccatct tcccgcagct
     2821 caaaatcctc tttcttcaaa tcgccgagga tctgccccgc cttggtcttc accgtggcat
     2881 ccaccatcac caggttcacc gccacccgca tcaccgatgg gtccggctcc tgccccaaaa
     2941 acgtaactgg aaatagcaac gcaaagacca cagccaaacc cataaaacgc attaaggtca
     3001 ccggatttat agcagcggtt tatcccgccc ctttgtc
//
LOCUS       DASTPM010000311         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1193172, whole genome shotgun sequence.
ACCESSION   DASTPM010000311 DASTPM010000000
VERSION     DASTPM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193172"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFR42_05480"
     CDS             complement(<1..495)
                     /locus_tag="VFR42_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368637.1"
                     /translation="MARSICVVRLVLSLVAMDIFAIPSRGQLSPSQTDALSKLQSSAN
                     SAACSATDVSSCAQAAAKLMPVILGDSPMIENLRKLTDEIGGRVSGTPEMAKAVQWGV
                     AGFRAAGVDVHTEKYKLPVMWKEGATRLIITAPASAIAGGATLRVVSEGWAPGTTREG
                     IEANA"
     gene            complement(546..1370)
                     /gene="folP"
                     /locus_tag="VFR42_05485"
     CDS             complement(546..1370)
                     /gene="folP"
                     /locus_tag="VFR42_05485"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU0368638.1"
                     /translation="MRLGERTLVMGVLNITPDSFSDGGKFFDPKLAVKQALAMQRDGA
                     DIIDIGAESTRPGSEGISAAEELRRLLPVMQALRGKLKLPISVDTQKSAVAEIALGAG
                     AQLLNDVSGLRSDPALAKVAAKYNAPLILMHMRGKPRSMQKGPFARDVVKDVVGGLRK
                     SIAIARKAGVAAAQIVIDPGIGFGKSFPQNYELLAKLPALAKLGFPILVGTSRKGFLG
                     ATLARNGKSAPLNQRLWGTAATVAASILNGVHIVRVHDVAKMVQVARVTDCLLEQL"
     gene            complement(1450..1758)
                     /locus_tag="VFR42_05490"
     CDS             complement(1450..1758)
                     /locus_tag="VFR42_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368639.1"
                     /translation="MSKRGSDNLNVTCPCCQAKLVVDPVFGAVLSHEAPPKKGPDVDL
                     TQASSILQEQQRQREDKFADSWFQETNKEDILTKKFEEAMKKAKDAPAGKPIRDFDLD
                     "
     gene            complement(2030..2761)
                     /locus_tag="VFR42_05495"
     CDS             complement(2030..2761)
                     /locus_tag="VFR42_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368640.1"
                     /translation="MNELLSHFRTRHRWPRRAFPTSLLILAFGFTFAHAQKKESVSAL
                     QPDKGKFSLLVEGKTMGQEEFEIAPSGGGWMARGATTIKTEKGDTKVTGNLTLQADGA
                     PISYDWTSQTDKVNGAHILFANGIAKITLEMQGSRPYEQDLSFGTPLVAVLDNNLYHQ
                     YAVLARLYDWTKAGAQQLPVLIPQELTPGNITLESTGSANAGGKTYEGLRVNTGDLEI
                     LLLLDAKHRLMRIEVPAAKVSVVRE"
     gene            complement(2871..3446)
                     /locus_tag="VFR42_05500"
     CDS             complement(2871..3446)
                     /locus_tag="VFR42_05500"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0368641.1"
                     /translation="MTRIPITSVVALAGFMGSGKTTVGTQLARQLAWRFVDLDDLIET
                     ASGTRIAEFFERHGEPSFRVLEAAQLRGALGRAFELREPVVLALGGGTYAQHGAPEFL
                     RMQNVPVIWLDSPIETLLARCMTMTGRPLFRDETSFRALHAQRLPSYQLADFRVDSGR
                     DPSIVVADILRLGIFPKADPDSHLLVEEPRS"
BASE COUNT          784 a         1092 c         1060 g          746 t
ORIGIN      
        1 cgcgttcgct tcgatgcctt cgcgcgtcgt gcccggcgcc cagccctcgg agacgacgcg
       61 cagcgttgct ccgcccgcga tcgccgatgc tggcgcggtg atgatgagcc gcgtcgctcc
      121 ttctttccac atcaccggca acttgtactt ttccgtatgc acgtccacgc cggcagcgcg
      181 aaaccccgcg acgccccact gcaccgcttt agccatttcg ggagtgccgc taacccgccc
      241 tccgatttcg tcggtcagct tgcgcagatt ttcgatcatc ggcgagtcgc ccaaaatgac
      301 cggcatcagc ttcgccgccg cctgcgcgca cgaagacaca tcggtcgccg aacatgccgc
      361 gctgttcgcc gaagattgca gtttgctcag cgcgtccgtc tgcgaaggcg acagctgccc
      421 tcgagatgga attgcgaaga tgtccatcgc aacaaggctc agaactaacc gaacaacgca
      481 aatcgaacgt gccatgtacg cctcgcaaaa tcatgagaac tgcagcaagg gaaagggtcg
      541 ggagattata gctgctcaag cagacaatcc gtcacgcggg ctacttgcac cattttggcg
      601 acgtcgtgga cgcggacgat gtgcacgccg ttgaggatac tggcggcgac ggttgccgcg
      661 gtgccccaaa gtcgctggtt caagggagcg gacttaccgt ttctggcgag cgttgcgccg
      721 agaaaacctt tgcgtgaagt gccaaccaga atcgggaagc ccagcttcgc gagagcgggt
      781 agcttggcaa gcaattcgta gttctgcggg aagctcttgc caaagccgat gcccggatcg
      841 atgacgatct gtgctgctgc gactccggcc tttctggcga tcgcgatgga cttacgcagt
      901 ccgcctacaa catccttcac tacatcgcgc gcgaatggac ctttttgcat ggagcgcggc
      961 ttaccgcgca tgtgcatcag gataagcggc gcattgtatt tcgccgctac cttggccaga
     1021 gcaggatccg accgcaagcc ggagacgtca ttgagcaatt gcgcgcctgc cccgagcgcg
     1081 atttccgcga cggcggattt ctgcgtgtcc acggaaatgg gaagttttag tttgccgcgc
     1141 agcgcttgca tcaccggcag caaccggcgc aattcttccg cggctgaaat tccttccgat
     1201 ccggggcgcg tcgattccgc tccgatgtca atgatatccg ctccatcgcg ctgcatggcg
     1261 agcgcttgtt ttacggcaag tttcggatca aaaaactttc cgccatcgga aaaactatcg
     1321 ggcgtgatat tgagtacgcc catcaccagc gtgcgttcgc caagtcgcaa aattccagaa
     1381 cgaagccgaa gcctgaagct tcctcgtaga cacgagtcac ccgtcacgaa tcaccagaca
     1441 ctgagctctt caatccagat cgaagtcgcg gatgggtttt ccggcgggcg cgtctttggc
     1501 cttcttcatg gcctcttcaa acttcttggt caggatgtct tccttattgg tttcctggaa
     1561 ccacgaatcg gcaaacttgt cttcgcgctg ccgctgctgt tcctgcagga tgctggaggc
     1621 ctgcgtcaaa tccacgtccg ggcccttttt cggaggcgct tcgtgcgaga gcaccgcgcc
     1681 aaaaacggga tccactacca gcttggcctg gcagcacggg cacgttacgt tcaagttgtc
     1741 gctgcctcgt ttgctcatgc ccgcattgta cgacggtgct gaccgtcggg gaagggcgca
     1801 agcgagctgc gcaaccgtgc cacccaaccc atcccaactg acgccgcatt gccgaccccg
     1861 gcggccacct gtaagttctt tatttccaac acttgcaaac tatatgaaaa caaatgactt
     1921 taagcatctt tagagtcagc agtccgcaaa acagggaggg ggcgccagca accggctacg
     1981 ctatgtaggc caaaaatcct ataaccaagt gcagtttttg ctcagcgatt cactcgcgaa
     2041 ccacagaaac cttcgccgcg ggaacctcaa tccgcatcag tcggtgtttc gcgtccagca
     2101 gcagaagaat ctccaggtcg cccgtgttca cccgcaaacc ctcataggtc tttccgcccg
     2161 cattcgccga tccagtcgat tccagcgtga tgtttcccgg agtcagttcc tgcggaatca
     2221 gcaccggcaa ttgctgtgct ccagctttcg tccaatcgta gagccgcgcc agcactgcgt
     2281 attggtgata gagattgttg tccagcaccg ctactagcgg cgtgccgaaa gaaaggtcct
     2341 gctcataggg ccgcgagcct tgcatttcca gcgtgatttt cgcgatgccg ttcgcgaaga
     2401 gaatgtgcgc gccgttgacc ttgtccgttt gtgaggtcca gtcatacgaa atcggcgcgc
     2461 cgtctgcttg cagcgtcaaa ttgccggtaa ctttggtatc gcccttttcg gttttgatcg
     2521 tcgtggcgcc acgggccatc cagccgccgc ccgagggagc aatctcaaac tcttcctgtc
     2581 ccatcgtttt gccttccacc agcagcgaga attttccctt gtctggctgg agtgcagaga
     2641 cagactcttt tttctgcgcg tgtgcgaatg tgaatccgaa ggcaaggatg agaagggaag
     2701 tgggaaaagc gcggcgcggc cagcgatggc gagtgcgaaa gtgcgataac aattcgttca
     2761 tacgtccccc aacgaaaaga gccaacaaaa acaggctagg aaaccagatt gtcacgatac
     2821 gagcgcgcga agcggaaaaa gagcggcggt gttgcgtgtt gtgcaaacgg tcaggagcgc
     2881 ggctcttcga caaggagatg cgaatcggga tcggccttcg gaaatatgcc gagcctgaga
     2941 atatcggcga cgacgataga cggatcgcga ccgctgtcca cgcgaaaatc ggccagttga
     3001 taggaaggga gacgctgcgc gtgcaacgcg cgaaagctgg tttcgtcgcg aaacagcgga
     3061 cgtccggtca tggtcatgca acgcgcgagc aaagtctcga tcggcgaatc gagccagatg
     3121 accggaacgt tttgcatacg cagaaattcc ggcgcaccat gctgcgcgta ggtgcctccc
     3181 cccagcgcca gcacgaccgg ttcacgaagt tcaaatgcgc gtccaagcgc gccgcgcagt
     3241 tgcgccgctt cgagcacgcg aaacgacggt tcgccatgac gctcgaagaa ttccgcgatg
     3301 cgcgtgcccg acgccgtttc gatcaggtcg tcgagatcca caaaccgcca ggcaagttgc
     3361 cgcgccaact gtgtgccgac agtggtcttg ccagagccca tgaagccggc caatgccaca
     3421 acggaagtga tgggaatacg agtcacgagg gaagtttaca gttgaggcga caaagttgga
     3481 aggcgctgcg ccgtgctgcc ggcgatcaga ggccagaaat cggcatcgct gcgttagtcg
     3541 cgcttgagaa tgcggatggt accgctgaaa gaggaaagct caactttggc gaggccgctg
     3601 cctacggagc cgaaaaggcc tttggcatag cgcgtcgcgg tgtggcggat gccgtgtgag
     3661 tcgggcttaa gaaacgcttc gg
//
LOCUS       DASTPM010000312         2355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_184044, whole genome shotgun sequence.
ACCESSION   DASTPM010000312 DASTPM010000000
VERSION     DASTPM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184044"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFR42_05505"
     CDS             <1..261
                     /locus_tag="VFR42_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368642.1"
                     /translation="VAWLAGTLFCGAETRSCPPRAEALKASRPITHQPSVRLTDFTFL
                     DSSFPMILGWAEAPIMITAESLLSLDAGLSAEETVNGRSRRK"
     gene            289..2307
                     /locus_tag="VFR42_05510"
     CDS             289..2307
                     /locus_tag="VFR42_05510"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEU0368643.1"
                     /translation="MNIRRCLRSFLMLFTLAACPLSARVTRVEIVSRADVLDGRSFGD
                     AGAYERVTGRVYFSAAVANAHNRSIVDLDKAGNLTDGEVEFSADFIAVRPKDAHKGNG
                     ALLLEVPNRGRGRIIALVDGGKWDISEDAGDGWLLRNGFTIVSLGWQWDADGPDALRL
                     YAPIAKENGKAINGLLRGDLMPSKLMDEIPLGHLITGNIGGTEYPVAAPNDPRNTLTV
                     RDSREAKRKVIPRSEWQFAHRVDGKLVPSDWHIHLSGGFQPGKIYEYVYVVKDPVVAG
                     LGFAAIRDFASYAKNNPDAITPARRVYGEGISQNGRFLRDLLYEGFNADEEGRIALDG
                     VLAHVAGAGRGSFNFRFAQPSRDAQPTSSVYFPTDTFPFTDLPEKDPVTHETGGLLDL
                     AISEKVVPKIFFSNTSYEYWGRAAALIHSTADGKRDVPISENVRIYHYTGLQHFSGPF
                     PPEKGSGDLLGQQPQSPLPVKFFWRAMIANMDAWVRDSTLPPPSSYPKISDHTLVSLL
                     AYAFPKIPEVNRPHEATTAYRLDFGPNWRQGILQWQPPKVGLLYPVLVPQVDADGNER
                     DGVRLPELSVPLATYASWNLRDPSIGAPSERVAFELSYLPFPKTAAERKNSGDPRKSI
                     AERYTGREDYVTRYAHAVDELIKQRWILPEDRDALLHRAAREWAEATK"
BASE COUNT          479 a          686 c          683 g          507 t
ORIGIN      
        1 gtggcttggc tggcggggac gctattttgc ggtgcggaaa cacgatcctg tccccctcga
       61 gccgaagcac tcaaagcgag caggcccatc acccatcaac ccagtgtgcg gctaactgat
      121 ttcacgtttc ttgactcctc attcccgatg attctgggct gggctgaggc tccaatcatg
      181 ataacagcgg aaagcctcct ttctttggat gccggtttga gcgccgaaga aaccgtaaac
      241 ggccgctcgc gccgaaaata attgcgctgc atgttatgct gccgggccat gaacatccgc
      301 cgttgtctca gatcgtttct gatgctgttt acgctggccg catgcccact ttcggcacga
      361 gtcactcgcg tcgaaattgt ctcgcgcgcg gatgtgctag acggcaggtc cttcggcgac
      421 gccggagctt acgaacgcgt cacggggcga gtgtattttt cggcggccgt cgccaatgca
      481 cataaccgga gcatcgtcga tctcgacaaa gccgggaacc tgacggacgg cgaggtcgaa
      541 ttctccgcag acttcatcgc cgtccgtccc aaggacgcgc ataaaggtaa cggcgcgctg
      601 ctgctggaag ttcccaatcg cggccgcggc cgcatcatcg ctctggttga cggcggcaag
      661 tgggatattt ccgaagatgc gggggacggg tggctgctgc gcaacggatt caccatcgtg
      721 tcgcttggat ggcagtggga cgcggacggg ccggatgcgc tgaggctcta tgcgccgatt
      781 gccaaggaaa acgggaaggc cattaacggg ttgctgcgtg gcgacctgat gccatccaaa
      841 ttgatggatg aaattccatt gggacatctg atcaccggga acattggcgg gacggaatat
      901 ccggtagccg cgccgaacga tccgcgcaac acgctcacgg taagggactc gcgcgaagcg
      961 aaacgaaagg tcattccacg ttcggaatgg caattcgcgc acagggtgga cggcaaactc
     1021 gtcccaagcg actggcacat tcatctgagc ggcggctttc agccgggaaa aatctacgaa
     1081 tatgtttacg ttgtgaagga tcccgtcgtc gcggggctgg gtttcgcggc gattcgcgac
     1141 tttgcttcct atgccaaaaa caatcctgac gcgattactc ctgcgcgacg cgtgtatggc
     1201 gaggggattt cgcagaacgg gcgcttcttg cgcgatttac tttatgaagg gttcaatgcc
     1261 gacgaagagg ggcgcatcgc gcttgacggt gtcctggcac acgttgcggg ggcgggccgc
     1321 ggcagtttta atttccgttt cgcgcaacct tcccgtgatg cacaacctac ttcgtccgtg
     1381 tatttcccta cggacacttt tccgttcacg gacctgccgg agaaagatcc ggtcacgcat
     1441 gaaaccggcg gactgctcga cctcgctatt tcagaaaagg tggtgccgaa gattttcttt
     1501 tccaatacgt cttacgagta ttggggacgc gccgccgcgc tgattcactc cactgctgat
     1561 ggcaaacgcg acgttccgat ttcggaaaac gtccgaatct atcattacac cggattgcag
     1621 cacttttccg gaccgttccc accggaaaaa ggctcgggtg atttgctggg ccaacaaccg
     1681 cagtctcctc tgccggtgaa atttttctgg cgggcgatga tcgccaacat ggatgcttgg
     1741 gttcgcgaca gcacgctgcc gccgccgagc agctatccaa aaatttcgga ccacacgctc
     1801 gtttcgctat tggcctatgc ttttcctaag atccccgaag tcaatcggcc gcatgaagcc
     1861 acaacggctt accgcctcga cttcggcccc aattggcggc aagggatctt gcaatggcag
     1921 ccgcccaaag tcggtctgct ttatccggtg ctggtgccgc aggtggatgc cgacggcaac
     1981 gaacgcgatg gcgtgcgttt accggagctt tccgttccgt tagcgaccta tgcaagttgg
     2041 aaccttcggg acccttccat cggcgcgccc agtgaacgtg tggccttcga actttcttat
     2101 ttgccatttc ccaaaactgc cgctgagcgc aaaaattccg gcgatccgcg caagtcaatt
     2161 gctgagcgct acaccgggcg cgaagactac gtcacccggt acgcccacgc tgtagacgaa
     2221 ttaattaaac aacgctggat cctgccggaa gaccgcgatg cgctgctcca tcgcgccgcg
     2281 cgggagtggg ccgaagccac gaaatagcct ttgtttcgat caggaagctt ggcccgcttt
     2341 cgccgctcgg cccgg
//
LOCUS       DASTPM010000313         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1250906, whole genome shotgun sequence.
ACCESSION   DASTPM010000313 DASTPM010000000
VERSION     DASTPM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250906"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFR42_05515"
     CDS             complement(<1..215)
                     /locus_tag="VFR42_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368644.1"
                     /translation="MHRSIAALLAAPSCDNAPPADHARSLRRNFGIGERDAMSAAGTN
                     PAAEADRQTAMAIARPGNGTMIFFSQFG"
     gene            complement(489..1367)
                     /locus_tag="VFR42_05520"
     CDS             complement(489..1367)
                     /locus_tag="VFR42_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368645.1"
                     /translation="MRQPEVCKHQVCDHCGGRFGMVTHRWWGNKFCKRACKNAHRREN
                     HHTQSGWVIPRFFTFAGGACLAAAVAVATLTLLLASANAAPGEERPVAGSSLKFNKED
                     GTLYIDLSGPIVAGMADDLRAALGKYGTTLNRVVLFLASAGGQVEEGDRVIEALNEIK
                     LRHQLITVVAHGKLCASMCIRIFLQGEDCLAAATAAVTPASTAASYIVPKHLLERLIV
                     GRHVQLVEVRKGTINNARFPHASHRFNPLFRDRSGLNSGFRLPGKQHIRSNKRQRQHL
                     PRGRGTCRMRRLSFDF"
BASE COUNT          532 a          768 c          801 g          548 t
ORIGIN      
        1 ccgaactgcg aaaaaaagat catggtgcca ttccccggcc gcgcgatggc cattgccgtc
       61 tgtctgtccg cctcggcggc gggattggtg ccagccgcgc tcatcgcatc gcgttcgcca
      121 atgccgaaat ttctgcgcaa gctgcgggcg tgatcggcag gcggagcgtt gtcgcaggac
      181 ggcgccgcaa gcaaggcggc aatgctgcgg tgcatatgtc gcctattggc acaaagcgga
      241 cttagcgaga catctgctcg tttgtccgct tttggggcga agcggacatt caaggccgtg
      301 cggctttgac ctcgtcggtc gctaatgacc cacaagcgac atcagcggga ttaaaattcc
      361 gcagcgcaga gtctcctgcc gttgtcgctt ccgaaaactt catccatcgg cctcgcggtt
      421 ggcagcagct gcgtggattg aagccccggc ggctttgcgg catgtcaccg ccggggctag
      481 tcgggccttc agaagtcgaa gctcagccgc cgcatcctgc aggtccctcg tccacggggc
      541 aggtgctggc gctgacgctt gttgctccga atgtgttgct tgccggggag gcggaagccg
      601 gaattgaggc cagagcgatc ccggaagaga ggattaaagc gatgagacgc atggggaaac
      661 ctcgcgttgt tgatggtgcc tttgcgaacc tcgacgagct gaacatgccg cccaacgatc
      721 aatctttcca atagatgttt tggaacgata tacgaggcgg cggtggaagc tggtgtcacc
      781 gccgccgtag cggccgcgag acagtcctcg ccttggagga agatccgtat gcacatggag
      841 gcacacagct tgccgtgggc caccacggtg atgagttgat gccgtagctt gatctcgttg
      901 agggcctcga tgacacggtc gccctcctcg acctgaccac cagccgaggc gaggaagagg
      961 accacgcggt tcaaggtcgt tccatactta cccagcgcag cgcgcagatc gtccgccatt
     1021 ccggccacga tcggtcccga caaatcgatg tagagcgtgc cgtcctcctt gttgaacttg
     1081 agcgacgagc cggctactgg tcgctcttcg ccgggtgctg cgttggcgga cgcaagcagc
     1141 agcgtgagcg tggcaactgc aactgctgcg gcaagacagg cgccaccagc gaacgtgaaa
     1201 aaccgaggga tgacccaacc tgactgggtg tggtgatttt cgcggcggtg ggcgtttttg
     1261 cacgccctct tgcagaactt atttccccac caacgatgcg tcaccatgcc gaaacgcccg
     1321 ccacaatgat cgcatacttg gtgcttgcag acttccggct gtctcatcac gccctcgcaa
     1381 gacagttcga tgggacctcg ttaagatagc agccaaccga acagaagaag gcgatcgaga
     1441 acttctctcc aacgatcgcc tcgacggcaa atccattcgc cagtgcgaaa gagttctgac
     1501 actttgcccc cgcacagccg accacccgcg aggggtcgag acccatctgt ggcaggtact
     1561 tcaaagcgtc ggtggttatc atgacgccct cgcatgcttt tggagggcta gggcggtaac
     1621 cttgcagtta ccggcgtaac acttgtgatc gcagtaggag agccgcgttg ttagttctcg
     1681 caggtagctc tccccgattg cctcacagca caacgcacag aaagattgcg ggcggggtgt
     1741 cctgccattc accaagacaa atttcatatg ccgcctcccc gttcagtttc ttgggtctgg
     1801 actcgtgtcg cgagcaggca gcaaagaaaa ctccagatga ggagcctgca aagaaagttc
     1861 attggggagc ctcctcggtt gcaagaccgc tacatccgtc gtctcctgag actgcatcag
     1921 gtcgttgctt attccggcca gagcgggtgc agtcaggaat gcgcggttct ttttgtgcag
     1981 ttcggcaatg aaaagcgctt ttttgtgatg gccatcacat aacgaacgca gtgccagcgc
     2041 gcggccccct gcaccgtttc gcacttgagc gacaaataat cggtcatgtt ttcggagcgg
     2101 ggttatcgga tcgaaatcct gcagtgcagc agacgtttgc cgggcgcgcg ccttcgttag
     2161 gaacggccgg gggaagcctg tacgggcgcc ctagtgattg agcgccaatc agggcttcgc
     2221 tcaggatctg tttcacggtt cgggcgcagc gcccgcaccg catgttgcag ccgaggcaac
     2281 cgtagacttg atgtgcgctc agcggttgca ctcgtgctgc cgtcgccacg ttccgtatct
     2341 cgcggtcgct cagtacattg catgagcaga caatcattga gctgcctgcg cttgctgctt
     2401 cagaagaggc cttagcgttt gccatacatc gatggacact tcgcagaggg ggtccacaac
     2461 gtcgctacta cggcgggatt gcgcttccgg tactgaaatc acgagaccca acgcggcggg
     2521 caccctcagt agaaaatcgg tccgtacggt gcggcgtagc ctgatttcag acgttcgcaa
     2581 gcttgggcgt agctgctggc ggagatgcca taaggtgcag tccagcttgt tccgtaaggt
     2641 atccagtag
//
LOCUS       DASTPM010000314         3877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_299245, whole genome shotgun sequence.
ACCESSION   DASTPM010000314 DASTPM010000000
VERSION     DASTPM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299245"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2150
                     /locus_tag="VFR42_05525"
     CDS             <1..2150
                     /locus_tag="VFR42_05525"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0368646.1"
                     /translation="GVKAVEVVQQPGSEIYWAGDEIVAVAAVDEPTVRDAVRAVKVQY
                     EELPHLVIDSDEPPRNLPPDTSPLSLNDLQDMARNQVPGAQVVKEINERGITFKPEEA
                     PLDRIPPDIANAIKSAKQKPAEKKAPSWYKPAAAETQGDAEAAFRAPDTVVSEGIYGI
                     PVIVHCCLETHGAVAEWTDNDHLLMHASTQYVSGIPGQMAEVIGIPAANIREVQENIG
                     GGFGSKFGPDRWGIYSAKLSKKAGGAAVKMMLSRAEEFAVAGCRPSAYARVRVAAKKD
                     GTLTAWESFGWGTGGPGGGGSPPIPYLIRDRIPNYRTQWTAVVNNIGPARAWRAPNHP
                     QACLITMTALDDLAHTLGMNPLDFFKKNVELTGPRANIYMDEFPIADELMGWSKRWHP
                     REDKTSAPIKRGLGLSMHTWGGRGHQSECDLTIQPDGSVTIKMGTQDLGTGTRTAIMA
                     IAADTLGLTPQQINLLYGDTQYPPSGGSGGSTTIGGVSATTRRAAVDARDALFAKVAP
                     TLGATAEQLEAVKGQIRVVGQPSKALSWKDACAKIGAVPINAHGKNLGGRDPLMNSGV
                     GGVQMADVSVDTETGVVKVNKMVAVQDCGLIVSLETAESQCYGALIMGISYALYEEKI
                     MDRTTGQMLNANLEFYRLARIGDIGELVVKMMVNKYDSRGVIGLGEPPVVSPGAAISN
                     AVANAIGVRVPILPLTPERVLAALRGARKGESA"
     gene            2147..3166
                     /locus_tag="VFR42_05530"
     CDS             2147..3166
                     /locus_tag="VFR42_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0368647.1"
                     /translation="MNRFEYVSPNSVKQALGLLSANWGEAEILAGGTDLLALMKDYIV
                     TPKRVVNIKQLPGMHEIAAERDGLRIGTLVTLDRIADAAQIRRDYPALAQCVGDAASP
                     QIRNMATIGGNLCQRPRCWYFRNGLGLLPKTADGKSLVVEGDNRYHAILGNDGPASFV
                     SPSTIAPMLIAYGARLLINGPAGEREVDVGKFFQVPTSENEREHDLKPNELITAVVVP
                     AARDIRAANYEVRQRASFDWPLATAAVALQMNGSTVKSASIVMGAVAPVPWVSHEASQ
                     AIAGKSIDERTASAAGQAAVSKARPLRHNDYKVKLAAVAVKRALLTAAGHAVPDVHSA
                     KGGAA"
     gene            3163..3402
                     /locus_tag="VFR42_05535"
     CDS             3163..3402
                     /locus_tag="VFR42_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368648.1"
                     /translation="MIAKPERLRFDTKHPHLCSALRWKGLFIDAERDETVPPCNDGLF
                     WCMYTQTCIGPDGQLAEPGNCSNPSRKCHGTGKCH"
BASE COUNT          845 a         1050 c         1248 g          734 t
ORIGIN      
        1 cgggcgtgaa ggcggtggag gtcgtacagc agcccggcag cgaaatctat tgggcgggcg
       61 acgagatcgt cgcggtagcc gcggtggatg agccgaccgt gcgcgacgcg gtgcgggcgg
      121 tgaaagtgca atacgaagaa ctgccgcacc tggtcatcga ttcggacgag ccgccgcgaa
      181 atcttccgcc ggacacaagt ccgctgagcc tcaatgattt gcaggacatg gcgagaaacc
      241 aggtgccggg ggcgcaggtc gtcaaggaga tcaatgagcg cggcatcact ttcaagccgg
      301 aagaggcgcc gctcgaccgc ataccgcctg atatcgcgaa cgcgattaag tcagcgaagc
      361 agaagcccgc ggaaaagaag gctccgtcct ggtacaagcc tgcggccgcg gagacgcaag
      421 gagacgcaga agctgcattt cgagcgccgg acacggtggt gtcggaaggg atttatggaa
      481 taccggtgat cgtgcattgc tgcctggaaa cgcacggtgc ggtcgcggaa tggaccgaca
      541 acgatcactt gttgatgcac gcttccacgc aatatgtttc gggcataccc gggcagatgg
      601 cggaggtcat cggaattccc gcggcgaaca tccgcgaagt gcaggagaac atcggcggtg
      661 gcttcggcag caagtttgga cccgaccgct ggggaatcta cagcgcgaag ctctcaaaga
      721 aggccggcgg tgctgcggtg aaaatgatgc tcagccgcgc ggaggagttc gcggtagcgg
      781 gctgtaggcc atcggcgtat gcccgcgtgc gagtggcggc gaagaaagac ggcacactga
      841 ccgcgtggga atcgtttggc tggggcacgg gcggaccggg cgggggcggt tcgccaccga
      901 ttccctatct gattcgagat cgcatcccga actaccgaac gcaatggacg gcggtggtca
      961 ataacatcgg tccggcacgc gcgtggcgcg cccccaatca tccacaagcg tgcctgatca
     1021 ccatgaccgc gctggacgat ctggcgcaca cattgggaat gaatcccctc gactttttca
     1081 agaagaacgt cgaattgacc ggaccacggg cgaacatcta catggacgaa ttccccatcg
     1141 ccgacgagct gatgggttgg tccaagcgct ggcatccgcg agaagacaaa acgtctgcgc
     1201 ccatcaaacg cggactaggc ctctccatgc atacctgggg tgggcgcggg caccagagcg
     1261 aatgcgacct gacgatccag ccggacggat cggtgacgat caagatggga acgcaggatc
     1321 tgggtacagg aacgcggacg gcgatcatgg ccatcgcggc ggatactctg gggctgacgc
     1381 cgcaacaaat caatctgttg tatggcgata cgcagtatcc gccgtcgggt ggttctggcg
     1441 gcagcacgac gattggcggc gtttccgcga caacgcggcg cgcagcagtg gacgcgcgtg
     1501 atgctctttt cgccaaagtt gcgccgacgc tcggtgccac agccgaacag ttggaggcag
     1561 tcaagggcca aattcgcgtg gtgggccagc catccaaggc gctgagctgg aaggatgcct
     1621 gcgccaagat aggtgcggtg cctatcaacg cgcacggcaa aaacctcggc ggccgcgatc
     1681 cgctaatgaa cagcggcgtc ggaggcgtgc agatggcgga cgtatcggta gacacggaaa
     1741 cgggcgtcgt caaagtgaac aagatggttg cggtgcagga ctgcgggcta atcgtttcgc
     1801 tggagaccgc ggaatcccag tgctatggag cgctcattat gggcatcagc tatgcgctct
     1861 acgaagaaaa aatcatggac cggactactg ggcagatgtt gaacgcgaac ctcgagtttt
     1921 atcggctggc gcgtatcgga gatatcgggg agctggtcgt gaagatgatg gtgaacaaat
     1981 acgacagccg cggggtaatc gggctgggag agcctccggt cgtttcacca ggcgcggcga
     2041 tcagcaacgc cgtggccaac gcgattggcg tgcgcgtacc gattttgccg ctgacgcccg
     2101 agcgcgtgtt ggcggccttg cgcggcgcgc ggaaggggga aagcgcatga atcgcttcga
     2161 gtatgtgagc cccaactcag tgaaacaggc cctcgggctg ctgtcggcca attggggcga
     2221 agctgagatt ctggccggcg ggaccgatct gctggcgctc atgaaagatt acattgtgac
     2281 acccaagcgg gtggtcaaca tcaagcagct tcctgggatg cacgagatcg ccgcggaacg
     2341 cgatgggttg cgtatcggca cattggtcac gttggaccgc atcgcagacg cagcgcagat
     2401 ccgccgcgat tatcccgcac ttgcgcaatg tgtaggcgac gccgccagcc cgcaaatccg
     2461 caacatggcc acgattgggg ggaacttgtg tcagcgtcca cgctgctggt atttccgcaa
     2521 cggactgggt ttgttgccca agacagcgga tgggaagtcg ctcgtggttg agggcgacaa
     2581 tcgctatcac gcaattcttg gcaacgatgg acccgcatct tttgtgagtc cctcaaccat
     2641 tgcgccaatg ctgattgcct atggcgcgcg gctgctcatt aatggcccgg ccggggagcg
     2701 cgaagtggat gttggaaagt tctttcaggt tccgacctcg gaaaatgagc gtgagcacga
     2761 tttgaagccg aatgagctga ttaccgcggt agtcgttccg gctgcaagag acatacgcgc
     2821 agcaaactac gaggtacggc agagagcctc ttttgattgg cctttggcca ctgcggcggt
     2881 tgcactgcaa atgaacggaa gcacagtcaa gagcgcaagc atcgtcatgg gtgcggttgc
     2941 gccggtaccc tgggtttcgc acgaagcctc gcaagcgatt gccggcaaat ccattgacga
     3001 gcgcactgcg agcgccgccg gccaagcagc cgtaagcaag gctcgtccgt tgcgtcacaa
     3061 cgattacaaa gtaaagttgg cggcagtggc ggtgaagcgc gcgctactga ccgcagccgg
     3121 tcacgcagtt cccgacgtgc acagcgcaaa agggggtgcg gcatgattgc caaaccagag
     3181 cggttgcgat ttgataccaa acatcctcat ctgtgctcgg cgcttcgctg gaagggactt
     3241 ttcatcgatg cggaacgcga tgagaccgtg ccgccatgca atgacggttt gttttggtgc
     3301 atgtacacgc agacgtgcat cggtcctgac ggtcaactcg ccgagccggg taactgcagc
     3361 aatccgtcgc ggaagtgcca cggcacgggc aaatgccatt gaagcgtgcg cgcactctcc
     3421 gtggttcttt ctctgacatc ctctgggctt tattccctat tctgggctcg ttgttgctcc
     3481 aataaagcaa ttcgaggcgc cccgacgtgt acacgcacgc tgagtggccg gagaacgtcg
     3541 aagctgcgaa tctggcggga gaccaaatcg aaaaagcggt gaattctgtc agcttaactg
     3601 caaataaaga aaaagggcct ctggttggag caccagaagc ccttgcagct taacaactta
     3661 attgggggtc agatttgaac tgacgacctt cgggttaacg catcttcatc gacgccttgt
     3721 ctctccaagt agagcgatca cgacggttcc ttgaaaagct tgtgtttgcc tttcaataat
     3781 ttacgttgtg cttgtattac ggtatttcgt gctgttctcg cgtgttctgt tcgtgagctg
     3841 ttccacgagt tgttcgcatg tcggaatcag acaattc
//
LOCUS       DASTPM010000315         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1077680, whole genome shotgun sequence.
ACCESSION   DASTPM010000315 DASTPM010000000
VERSION     DASTPM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077680"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="VFR42_05540"
     CDS             complement(<1..1016)
                     /locus_tag="VFR42_05540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0368649.1"
                     /translation="MSEGVQVSPAATAELSLPHRPPASPPAEVSSPISINRWVYALAA
                     FATLFHLVFSMRYGFFRDELYYAACGQHLAWGYVDHAPLAALIARISRAFFGDSLFAL
                     RFVPALAAAAKVYLAGWMARELNGDRFAQLLAALTALVAPIYLTFDSFLSMNSFEPVF
                     WMACAAVLLRILNGGSEKLWLLFGLIAGIGILNKHSMLFFGSGVFAALLLTPARREFA
                     KKYIWFGGAIAFLIFLPNLLWEINHGWPTISLLHKVIGTKYTSIAPWEYIWQQSLLTH
                     PVATPIWLVGLCYFLFDRAGRKYAALGLAYLIVLAEMIALHGKIYYLAPAYLMLFAAG
                     AVWS"
     gene            complement(1178..3055)
                     /locus_tag="VFR42_05545"
     CDS             complement(1178..3055)
                     /locus_tag="VFR42_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0368650.1"
                     /translation="MNILDLLVGKPLRTSDERAEKIGSSRGVPIFGLDGLSSAAYGPE
                     AALSLLIPLSLAGVRYIIPISTAIIALLVIVYFSYRQTIAAYPSGGGSYTVARFNLGA
                     PAGLLAAAALLADYILTAAVGISAGVGALVSAVPALLPHTVSICMTILVIVTVLNLRG
                     VREAGLIFAVPTYLFVGTLLITIAAGLVRVLLSGGHPTPIAPLPPPPPITEAVSYWLL
                     LKVFASGCTALTGVEAVSNGVKAFREPAVKNAQRTLTIIILILAILLAGISYLVKVYG
                     IVATDPGQPGYQSLLSMLIAAVFGKGYFYYVTIGSILIVLSLSANTAFADFPRLCRAI
                     AQNNYLPHAFGYRGRRLVYSYGIVVLAVLCGALLMLFRGVTDRLIPLYAVGAFLAFTL
                     SQSGMVVHWWRNRGAHWRKSAFVNGLGAFVTGITVIVVLVAKFVEGAWITLIFIPLTI
                     AFFSAVRRHYHHVRALTTCRIPVHMPDRQQTPIAVIPIDRWSNITRQGIEFAARLSSE
                     VIALHVDPSEHSELLKEDWERYVVQPFREAGKQPPKLEILPSPYRFIIIPVVQFIVEL
                     SERHPDRRIIVVIPELVEDRWYEYFLHNQRGRLLEWVLLVHGNERIFTVSAPWYVHER
                     K"
     gene            3157..3759
                     /locus_tag="VFR42_05550"
     CDS             3157..3759
                     /locus_tag="VFR42_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368651.1"
                     /translation="MKAQPFAPQMRVYTPRMKCVALLMVLAFSASAQSPVDKPSDSET
                     SLAKKLDPKLHADAIKLVEVSDAKQRMQDSLEKMVDESQKQMMDKCPGCDPEFGKEWK
                     KRFIQRTNVNDYVDVWVRVYEKYFTDAEINELLALVKDKSKTPSAPLKEKLTAVMPSL
                     LADVMGDCAKIGAKLGAEIAAEIEHEHPEYVKPPANSATP"
BASE COUNT         1005 a          981 c         1188 g          702 t
ORIGIN      
        1 tcgctccaaa cggctcctgc cgcgaagagc atgagatagg ccggcgcgag gtaatagatt
       61 tttccgtgca gtgcaatcat ttctgccagc acgatcaaat aggccaagcc tagcgcagca
      121 tatttcctcc cggcgcgatc gaaaaggaag tagcagagtc cgacgagcca gattggcgtg
      181 gcgaccgggt gggtcaagag tgactgctgc cagatgtact cccatggtgc gatgctggtg
      241 tatttcgtgc cgatcacttt gtgtagtagc gatatggttg gccagccatg attgatttcc
      301 cagagtagat tggggagaaa aatcaggaag gcgatagcgc ctccgaacca gatatatttt
      361 tttgcaaatt cgcggcgcgc cggggtgagc aacagggcgg cgaatacccc tgagccaaaa
      421 aacagcatcg agtgcttgtt gaggattccg atccctgcaa tcaagccgaa cagcagccac
      481 aatttttcgc tgccgccatt tagaatgcgc aagaggacgg cggcacaggc catccagaag
      541 actggctcga aggaattcat agagaggaaa ctatcgaagg tgagatagat cggcgcgacc
      601 agcgcggtaa gagctgccag caattgggcg aaacgatctc cgttcagttc ccgggccatc
      661 cagcccgcca ggtacacctt ggccgccgcc gcgagggccg ggacaaagcg aagagcaaat
      721 agcgaatctc cgaaaaatgc gcggctgata cgcgcgatca gggccgccag tggcgcatga
      781 tcgacatagc cccaggccaa atgctggcca caggccgcgt aatacaattc gtcgcggaaa
      841 aatccgtaac gcatgctgaa aacaagatgg aaaagcgtgg cgaacgcggc cagcgcatag
      901 acccagcgat taatcgatat tggtgaagaa acttcagccg gtggtgaagc cggtggccga
      961 tgcgggagag atagctcggc cgttgccgcc ggggagacct ggacgccttc cgacaaagta
     1021 ccgcctgacg gcgtgtggtt gcgaccgtga gagtcgccgt gaactctcga aagctttgcg
     1081 aatcaatgac agtgtcgaga ttgtagcaga acccacgaac cagaaacgcg gatcgaatta
     1141 aaagtggtcg atcgattttg caacgcccga gaataagcta tttgcgttcg tggacgtacc
     1201 acggagcact tacagtgaag atgcgctcat tgccgtggac cagcagcacc cattcgagga
     1261 ggcggccgcg ctgattgtgg aggaaatatt cataccagcg gtcttcgacg agttcaggga
     1321 tgactacgat aatgcgtcgg tccggatgcc tttcggaaag ttcaacgata aattgcacga
     1381 caggaataat gatgaaacgg tagggcgaag gaaggatttc gagcttgggc ggctgtttgc
     1441 ccgcttcgcg gaagggctgt acgacgtagc gttcccaatc ttctttgagc agctccgagt
     1501 gctcggaggg gtcgacgtgc agagcgatca cttcgctgga aaggcgcgcg gcaaattcga
     1561 tgccctggcg ggtgatgttg ctccagcggt cgataggaat aacggcgatg ggagtttgct
     1621 gtcgatcggg catgtgcaca ggaatccggc aggtggtaag ggccctcaca tgatggtagt
     1681 ggcgacggac ggcggagaag aaggcaatcg tcagcggaat gaaaattaaa gtgatccacg
     1741 caccctcgac gaatttggcg accaggacga cgattaccgt gatcccggtg acgaatgccc
     1801 cgagaccatt aacgaacgca cttttgcgcc aatgcgcgcc gcgatttctc caccaatgca
     1861 cgaccatgcc ggattgcgaa agggtgaaag ccagaaacgc accgacggca tagagcggaa
     1921 tgagccggtc ggtaacgccg cggaagagca tcagcagcgc gccgcacagc accgcgagga
     1981 ctacgatgcc gtaggaatag accaaacggc gtccgcgata gccgaaggcg tggggcagat
     2041 aattgttttg cgcgatcgcc cggcacagcc gcggaaaatc cgcgaaggcg gtgttggcgg
     2101 aaagcgaaag tacgataagg atggagccga tggtcacata gtagaaataa cccttgccga
     2161 aaacggcagc gatcagcatg gagagcaggc tctggtagcc gggctggcca gggtcggtgg
     2221 cgacaatgcc gtacactttg acgagatacg aaattcccgc caggaggatc gccagaataa
     2281 gaatgattat cgtcagggtg cgctgcgcgt tttttacggc cggctcacgg aaggctttta
     2341 ccccgttgct gacagcctct acgccggtca aagcagtgca gccgctggcg aaaaccttca
     2401 gcagcaacca atagctgacc gcttcggtaa tgggcggcgg aggcggaagg ggcgcaatcg
     2461 gcgtgggatg tccgccgctg agcagcacgc ggaccaggcc ggcggcgatc gtgataagta
     2521 gggtgccaac aaaaagataa gtggggacgg cgaagatcag gccggcttcg cgcacgccgc
     2581 gaagattcag gacagtcaca attaccagta tggtcatgca gatcgaaacg gtgtgcggca
     2641 gcagcgcggg aaccgcggaa accagagcgc cgactcccgc ggagatgccc acggcagcgg
     2701 tcagaatata gtccgcgagg agcgcggcgg cagcgagcag gccggccggc gcgccgaggt
     2761 tgaagcgggc gacggtgtag gagccgccgc ccgagggata agcggcgatg gtctggcgat
     2821 acgaaaagta gacaatcacc agcaaggcga tgatggccgt gctgatggga atgatgtagc
     2881 gaacaccagc caggctcagc ggaatcagca ggctgagggc ggcttcaggt ccgtaggccg
     2941 cggaactcag gccatccaag ccgaaaatag gcacgccgcg ggaagaaccg attttctcgg
     3001 cacgctcatc actggttctc agaggtttgc cgactagaag gtcgaggatg ttcatgagtc
     3061 ggaggggcta ttcaactgga tgtgactgcc gactaggcag accacgataa caccgtagag
     3121 cttgcattca aaagtggcag cgttttggca acaactatga aagcacaacc cttcgctcca
     3181 caaatgaggg tgtatactcc gcgcatgaaa tgtgttgcct tgcttatggt tctggctttc
     3241 tcagcgagtg cacaatctcc cgttgataag ccctcggaca gtgagacctc tcttgctaag
     3301 aagcttgatc ccaaactcca cgcggatgca attaagctgg tcgaagtcag cgatgcgaaa
     3361 caacgcatgc aagatagcct cgaaaaaatg gttgatgaat cgcaaaaaca gatgatggac
     3421 aagtgtccgg gatgtgatcc tgagttcgga aaagaatgga aaaaacgatt tatccaacga
     3481 acaaacgtca acgactatgt ggatgtctgg gttcgcgtgt acgagaaata ttttacggat
     3541 gcagaaatca atgaattgct tgccctagtg aaagacaagt cgaagactcc ctcggccccg
     3601 ctgaaggaga aattgaccgc ggttatgccg tctcttttgg ccgacgtaat gggtgattgc
     3661 gccaaaatag gcgcgaaact cggggccgag atcgccgctg agattgagca cgagcacccg
     3721 gaatacgtga agcctcccgc aaattctgct acaccttagt gcaacacaat catcgcgttt
     3781 gatctcaaga ggcgtttaga cgatatccat ctcgaaggtc gcaataaagc ctacgacacg
     3841 ggatatgtcg ccaaaaaacg gaagtcactc tatgca
//
LOCUS       DASTPM010000316         7402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_299385, whole genome shotgun sequence.
ACCESSION   DASTPM010000316 DASTPM010000000
VERSION     DASTPM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299385"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            478..1110
                     /locus_tag="VFR42_05555"
     CDS             478..1110
                     /locus_tag="VFR42_05555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU0368652.1"
                     /translation="MKRRELLRAGAFAGAVAALRPPVGQAQSQTQNQVQGQELTPAQS
                     GVDASNDLAAPGWKPRFFDEHQNETLIVLSDLIIPATDTPGAKEALANRYIDLVLAAE
                     NPEGQRAFLNSLSYLDGESMRRYKAAFRYLKREDQDDLLHALAYPKAGSGWTGEAASA
                     DVGHTHFEFLKQRIAGAYYSSAIGEKELGWDGAFAHGPYKGCDHAEGNHK"
     gene            1107..2843
                     /locus_tag="VFR42_05560"
     CDS             1107..2843
                     /locus_tag="VFR42_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0368653.1"
                     /translation="MKGIYKGGTASGSEGGLYGLEDPLTQTYDVIVVGSGATGGWAAK
                     QMAEAGLKVALLEAGRAVSPKEFTEHMPAYKLKYRDHSPEIARTRPVQKQCYACMEYN
                     YEWFVNDLENPYSTPQGKPFTWQRLRILGGRSLVWGRQSYRMSDNDFKAASRDGYGDD
                     WPISYADVAPYYDIVEKYVGITGAAEGNEMLPDGQFLPPMKMSCGEILMRDRVKAKFG
                     RTVTIGRAAVLTQNHNGRLGCHYCGPCERGCSTFSYFSSPFTTVKDAVASGNCALITN
                     AVVSHVDMDNATNKARGVTYLDRLTRQVKEVKGKSVILCAQALESTRILLNSSTREHP
                     NGLGNSSGALGHYLMDHVVGGGAYGKLPDMKGQADANEPQRPNGIYVPRFRNTPTSGK
                     HSRFIRGYGFQGGADMDFDFGAEGYGTTFKNAVKEGAYRISLGGFGESLARWDNYVEI
                     DKDLKDAWGIPALRISMTHGDNEAALMEDAAATAAEMLEATGAKDIKLQANVEMPGMA
                     IHELGTARMGTDPKRSVVNPFNQTHDVKNVFVMDGATFVSSACQNPTLSMMAITVRAC
                     ANLIDRFHKNDV"
     gene            complement(2870..3529)
                     /locus_tag="VFR42_05565"
     CDS             complement(2870..3529)
                     /locus_tag="VFR42_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368654.1"
                     /translation="MSLSRSIVLCILVAVLFFAGFAWKRYPSLGQPREASKPIIEKQP
                     VVFSKRSFDPAAPADDMPPLGEGEEAECDSNFISDANVSGRMQKIDATNATVTVTQVK
                     VTLQLKITIWVPNGSTQHVIEHEQGHRQISEHYYELADKVAAEITAPYIGRQIAISGS
                     DLNAEFRNALLQLSKQITAEYNDKLTPGPAQQRYEDLTDHSRNDRDANDAAAQAIKET
                     M"
     gene            3712..>7402
                     /locus_tag="VFR42_05570"
     CDS             3712..>7402
                     /locus_tag="VFR42_05570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0368655.1"
                     /translation="MDAVSASPLSTDSRSSLEWAHPLVRDWFVNRFGTATEPQLEGWP
                     RILAGETTLISAPTGSGKTLAAFLICIDRLVRKAIAGDLLDRTEVLYVSPLKALSNDI
                     QKNLETPLGEIFQLAGERGILMPEIRTSVRTGDTLAHERRAMLARPPHILVTTPESLY
                     ILLTAARSREILRTVDSVIVDEIHAMADDKRGAHLALSLERLDHLTGKRLTRIGLSAT
                     QKPIELVAHFLTGNDQPEPAVVQIGHRRQMDLAVEVPGDELGPVASNEMWGETYDRLA
                     ELAKQHRSTLIFVNTRRLAERVSHHLAERLGNEAVAAHHGSLSRKLRLHAEKRLKSGE
                     LRALVATASLELGIDIGAVDLVCQIGSPRSISVALQRIGRAGHWRGAISKGRLFATTR
                     DELVECAALVRAIHQGDLDRIAIPETALDILAQQIVAMCAAEDWKEDELFAVTRRAYP
                     YAGLTRATFDEIVEMLSEGIASARGRYGAYLHRDRVNGMLRARRGSRLAAITSGGAIP
                     ENALYTVIAQPEGTVVGTIDEDFAVESLAGDVMLLGNTSWRIRRVQAGSVLVEDAQGA
                     APTIPFWRGEAPARTDELSAHVGELREKLSGLLANVAPSADGKLPSEAAAAVDWLKFE
                     CGVDEAGAEQIVEYILEGRAVLGAVPTQTTVIAERFFDESGGMQLVIHAPFGGRINKA
                     WGLALRKRFCRSFNFELQAAATDNGLNISLSEQHSFPLADVFHFLHPNSARTVLAQAV
                     LASPYFTTRWRWDAGRALALLRFRGGKKVPPNIQRMRADDLLASVFPEQQACPENLAA
                     GDIEIPNHPLIREVMKDVLTEAMDVDGLERVLRGINDGSIQCLAVDTPVASQFSHEIL
                     NANPYAYLDDAPLEERRARAVEMRRVLPQAVLEEIGKLDPAAIEEVCEEAWPDVRDAE
                     ELHDALLTLIAIPASREHVNTSNRVRSRLENSLPSWTPHFERLVAERRTGIVRVGQAS
                     YWVSAERVAAFRQIFPAAQFEPEMPTLDAREISREDAVLACITGWMTHSGPVSANQLS
                     DLLGASVGEVEKTLLRLESRGVILRGDFTGAVTSPGDTGSIEWCERRLLARIHRLTLG
                     RLRKEVQSVTPAQFMDWLLSWQHVAPGTQLLGERGTLEAIRQMQGFEAPANAWERQIL
                     LRRIADYDGKVLDQLCLTGAVGWGRLSPHPATLETIADGKRRVIPTSVAPITFFVRED
                     AEWMIPRREDAEALPSGLNADASAVL"
BASE COUNT         1526 a         1755 c         2651 g         1470 t
ORIGIN      
        1 gtgcacgggg catacgcttc ttacgaagaa aacatgaaag ggtcgctgga agcgggcaaa
       61 ctggcggacc tggtggtgtt gggccgcgat ccgatgaagg aagagccctc cacgctgatt
      121 acgattccgg tggagcgcac gatggcggac gggcgttgga cttacgaatc gtaaaacgat
      181 ttgctgagtc gacattggtg tagactttcg agggccagga cctcagtaag tccggtggat
      241 ggatgtagtg agaatctgct gagtcacttt caacatgcga ctggagccgt tagtagtact
      301 ggaaattcgc ggtcgtacaa gtttgaagag acaaatcctc aggggctaaa cccagtctat
      361 ttgccaagcc caaatgtcgg agctaagctc cgactcctga caaagcagta gcgaatggca
      421 agaggttaaa agatttacag gcggcgctac aagagaggtt gagggaggct ggtttccatg
      481 aaacgacggg aattgttgcg tgctggagcg tttgcgggag cggtggcggc gctgcggccg
      541 ccggtcgggc aggcgcaaag ccagactcag aaccaggtgc aggggcaaga gctgacgccg
      601 gcgcaaagcg gagtggatgc ttcgaacgac ttagccgcgc cgggatggaa gccgcgattt
      661 ttcgacgaac atcagaatga gacgctgatt gtgctgagcg atttgattat tccggcgaca
      721 gacacgccgg gcgcgaagga agcgctggcg aatcgctaca ttgatttagt gctggcggcg
      781 gaaaatcccg agggtcagag agcgtttttg aacagcttga gttatctgga cggggagagc
      841 atgcggcgct acaaggcggc atttcgttac ttgaagcgcg aggaccagga cgatttgctg
      901 catgcgctgg cgtatccgaa ggcgggcagc ggatggaccg gagaagcggc aagcgccgac
      961 gttggtcata cacattttga atttttgaag cagcgaatcg ccggagcgta ttacagctcg
     1021 gcgattggtg agaaggaatt gggatgggac ggcgcgtttg cgcatggacc atataagggt
     1081 tgcgatcacg cggaggggaa ccacaaatga agggaatcta caaaggtgga actgcgtccg
     1141 gctcagaagg cggcctgtac gggctggaag atccgctgac gcaaacgtat gacgtgattg
     1201 tggtgggctc gggtgcgacg ggcggctggg cggccaagca gatggccgaa gccgggttga
     1261 aagtggcgct gctggaggcg gggcgcgcgg tgagtccgaa ggaattcacc gagcacatgc
     1321 cggcgtacaa attgaaatat cgtgatcatt cgccggaaat tgcgcgcacg cggccggtgc
     1381 agaagcagtg ctacgcgtgc atggagtaca actacgaatg gttcgtgaac gatctggaga
     1441 atccgtacag cacgccgcag ggaaaaccgt tcacgtggca gcggctgcgg attctggggg
     1501 ggcggtcgct ggtgtggggg cggcagagct accgcatgag tgacaacgac tttaaggcgg
     1561 cgagccgcga tggttacggc gatgactggc cgatttcgta cgcggatgtc gcaccgtatt
     1621 acgacatcgt ggagaaatac gtcggcatta cgggcgcggc ggaaggcaat gaaatgctgc
     1681 cggacgggca gtttttgccg ccgatgaaga tgagctgcgg cgaaatccta atgcgggacc
     1741 gcgtgaaagc aaaattcggg cgcacggtga cgatcggacg tgcggcggtg ctgacgcaga
     1801 atcataacgg gcggttgggt tgccactatt gcgggccgtg cgagcgggga tgttcgacat
     1861 tttcgtattt cagcagtccg ttcacgacgg taaaggacgc ggtggcgtcg ggaaattgcg
     1921 cgctcatcac gaacgcagtg gtgagtcacg tggatatgga caacgcgacc aacaaggcgc
     1981 gcggcgtgac gtatctagac cggttgacgc ggcaggtaaa agaggtgaag ggcaagtcgg
     2041 tgattctgtg cgcgcaggcg ctggagtcga cgcggatcct actgaactct tcgacacggg
     2101 agcatccgaa cgggctgggg aattcgagcg gggcgctggg acattacttg atggaccacg
     2161 tggtgggcgg cggagcgtat ggcaaattgc ctgacatgaa gggccaggcc gatgcgaacg
     2221 agccgcaacg tccaaatgga atctatgttc cgcgctttcg caacacgcca acgagcggga
     2281 aacattcgcg gtttattcgc ggctatggat ttcagggcgg cgcggacatg gattttgact
     2341 tcggtgccga aggatacggg acgacgttca agaacgcggt gaaagaaggc gcatatcgga
     2401 tttcgctggg cgggttcggc gaatcgctgg cgcgctggga caactacgtg gagattgaca
     2461 aggatctgaa ggatgcgtgg ggaattccgg cgctgcggat ttcgatgacg cacggcgaca
     2521 acgaagcggc gctgatggaa gacgcggcgg caacggcggc ggaaatgctg gaggcgacag
     2581 gcgcgaaaga catcaagctg caggccaacg tggagatgcc aggaatggcg attcacgagc
     2641 tggggacggc acggatgggc accgatccga aaaggtcggt ggtgaatccg ttcaatcaga
     2701 cgcacgacgt gaagaatgtg tttgtgatgg acggagcgac tttcgtgtcg tcggcgtgcc
     2761 agaacccgac gctaagcatg atggcgatta cggtgcgggc gtgcgcaaac ctgattgacc
     2821 ggtttcacaa gaacgacgtt taacgccggg tgggttgcgg agttcccaat tacattgttt
     2881 ctttgatggc ctgagcggca gcgtcgttgg cgtcgcgatc gttgcggctg tggtcggtga
     2941 gatcctcgta acgctgctgg gctgggccgg gagtgagttt gtcgttgtat tctgcggtga
     3001 tctgcttact gagctgcaag agagcgttgc ggaattcggc gttcaagtca gatccgctga
     3061 tggcgatctg ccggccgatg tagggcgcgg taatttccgc ggcgaccttg tcggcgagct
     3121 catagtagtg ttctgaaatt tgacgatggc cttgctcgtg ttcgatgaca tgctgcgtgg
     3181 agccattggg aacccaaatt gtaattttga gctgcagagt cactttcacc tgagtgacgg
     3241 tgacggttgc gttggtggcg tcaatttttt gcatgcggcc gctgacgttg gcatcggaaa
     3301 tgaaattgga gtcgcactcg gcttcttcac cttcgccgag tggcggcata tcgtcggcag
     3361 gcgcggcggg gtcgaaactg cgtttcgaaa aaacgaccgg ttgcttctcg atgatgggct
     3421 tggaggcttc ccgcggctgc ccgagcgaag ggtagcgttt ccaggcgaaa ccggcgaaga
     3481 acagtacggc gaccaagatg cataggacga tggaacggga aagggacacg cggcctccgc
     3541 gttctatttt ccgagagcgc gtatggcggg ggccacgaaa tagtgttgcg cgattgccgc
     3601 gagagtcacg gatgcgtgca cgcgttttat cgaagaaaag cagcggctaa agagcggtgg
     3661 agggcttgca agcggcggaa ctgcggcact gtataataag gatgcaaggc tatggacgca
     3721 gtttccgcat cgccgctgtc cactgactca cgatcgagcc tggaatgggc gcacccgctt
     3781 gtgcgggact ggtttgtcaa ccgattcggc actgccacag aaccacagtt agaaggctgg
     3841 ccgcggatcc tggcgggcga gacgacactg atttcggctc cgactggttc gggaaaaacg
     3901 ctggcggcat tcttgatttg cattgaccgg ctggtgcgga aggcgattgc cggagacttg
     3961 ctggatcgca cggaagttct ctacgtctca cccctaaaag cgctcagcaa cgacattcag
     4021 aaaaatctgg agactccgct gggggagatt tttcagctgg cgggggagcg cggaattttg
     4081 atgccggaga ttcgcacgtc ggtgcggacc ggcgatacgc tggcgcacga gcggcgcgcg
     4141 atgctggcgc gtccgccgca cattctggtg accacgccgg agtcactcta cattcttttg
     4201 accgctgcaa ggagccggga aattttgcgg acggttgact ccgtgattgt ggacgaaatt
     4261 catgcaatgg cagatgacaa gcgcggtgcg cacctggcgt tgtcattgga acggcttgac
     4321 catttgactg gcaagcggct gacgcggatc ggcctttcgg ctacgcaaaa accgattgag
     4381 ttggtggcac attttctgac gggcaatgac cagccggaac cggcggtggt gcagatcggg
     4441 caccggcgcc agatggattt ggcggtggag gttccggggg atgagctggg gccggtcgct
     4501 tcgaacgaaa tgtggggtga gacttacgac cggttggcgg agttggcgaa acagcatcgg
     4561 tcgacgttga tttttgtgaa tacgcggcgg ctggcggagc gggtgtcgca tcacctggcg
     4621 gagcgtttgg ggaatgaggc agtggcggcg catcacggca gtttatcgcg caagctgcgg
     4681 ctgcatgcgg agaagaggct aaagtccggc gaactgcggg cgctggtggc gacggcttcg
     4741 ctggagctgg gaattgatat tggcgcggtg gatctggttt gccagatcgg gtcaccgcgt
     4801 tcgatttcag tggcgttgca gcggattgga cgcgcggggc actggcgcgg tgcgatttcg
     4861 aaggggcggc tgttcgcgac gacgcgcgac gaattagtgg agtgcgcggc gttggtgcgg
     4921 gcgattcatc aaggcgattt ggaccggatt gcgatccctg agactgcgct ggatatattg
     4981 gcgcaacaga ttgtggcgat gtgcgcggcg gaggattgga aggaagacga attgtttgcg
     5041 gtgacgcggc gggcctatcc ctacgccggg ctgacgcgag cgacgttcga tgaaattgtc
     5101 gagatgcttt cggagggcat tgccagcgcg cgcgggcgtt atggagcgta tctgcaccgc
     5161 gaccgcgtga atggaatgct gcgcgcgcgg cgtggaagcc ggctggcggc gattaccagc
     5221 ggcggtgcga ttccggagaa tgcgctgtac acggtgattg cgcagccgga aggaacagta
     5281 gttggcacga ttgacgagga tttcgcggtg gaaagtttgg ccggcgacgt gatgctgctg
     5341 gggaatactt cgtggcggat ccggcgcgtg caagcaggca gcgttctggt agaggatgcg
     5401 cagggcgcgg cgcctacgat tccattttgg cgaggtgaag cgccggcgag aacggatgag
     5461 ctttcggcgc atgtgggtga attgcgggag aagctcagcg ggctgctggc gaatgtcgct
     5521 ccgagtgccg atggaaaact gccgagtgag gcggctgctg cagtggactg gctgaagttc
     5581 gaatgcggtg tggacgaggc tggggccgag caaattgtgg aatacattct ggaagggcgg
     5641 gcggttttgg gagctgtgcc gacgcaaacg accgtgattg cggaacggtt ttttgatgaa
     5701 agcggcggga tgcagctggt gattcacgcg ccgttcgggg gacggatcaa caaggcgtgg
     5761 gggctggcgc tgcggaagag gttttgccgg tcgttcaatt tcgaattgca ggcggcggcg
     5821 acggacaacg gattaaatat ttcgttgagc gagcagcaca gttttccgct ggcggacgtg
     5881 tttcatttcc tgcatccgaa ctccgcgcgg acggtgctgg cgcaggcggt actggcttca
     5941 ccgtatttca caacgcgctg gcggtgggat gcgggtcgag cgctggcgct gctacgcttt
     6001 cgcggcggga aaaaagtgcc gccgaatatt caacggatgc gcgcggatga tttgctggcg
     6061 tcggtgtttc cggagcagca ggcttgtccg gaaaatctgg cggctggcga cattgaaatt
     6121 cccaaccatc cgctgatccg cgaagtgatg aaggatgttt tgacggaggc gatggatgtt
     6181 gatggcctgg agcgcgtgct gcgcggcatc aacgacggca gcatccagtg tctggcggtg
     6241 gatacgccgg tggcctcgca gttttcgcac gaaattctga acgcgaatcc ctatgcgtat
     6301 ctggatgatg cgccgctgga agaacggcga gcccgggcgg tggaaatgcg ccgcgtcttg
     6361 ccgcaggcag tgctggaaga aatcggcaag ctcgatccgg cggcaattga agaagtttgc
     6421 gaagaggcgt ggccggatgt gcgcgacgcg gaagaattgc acgatgcatt gctgacattg
     6481 attgcgattc ccgctagccg agagcacgta aacacaagca atcgcgtgcg gtcgaggctg
     6541 gagaattccc tgccctcctg gacaccgcat tttgagcgac tggtggcgga acggcgcacg
     6601 gggattgtac gggtgggcca ggcaagttac tgggtttcgg cggagcgagt tgcggcgttt
     6661 cgacaaattt tccctgcggc gcagtttgag ccggagatgc cgacgctcga tgcgcgagag
     6721 atttcgcgcg aagacgccgt gctggcgtgc atcaccgggt ggatgacgca ctcgggacca
     6781 gtgtcggcga atcagctgag cgacttgctc ggcgcaagcg ttggcgaggt cgaaaagacg
     6841 ctgttacggc tggagtcgcg cggcgtgatt ttgcgcggcg attttacggg agcggtgacg
     6901 tcaccaggcg acacaggttc catcgaatgg tgcgaacgca ggctgctggc gcggattcac
     6961 cggctgacgc tggggcggct gcgcaaggaa gtgcagtcgg ttacgcctgc tcaatttatg
     7021 gattggttgc tatcgtggca gcacgtggca ccgggaaccc aactgcttgg ggaacgtgga
     7081 acgctggaag caattcgcca gatgcagggg ttcgaagcgc cggcgaacgc ctgggagcgg
     7141 cagatactgc tgcggcgcat cgcggattat gacgggaagg tcttggatca actgtgcctt
     7201 actggagcgg tgggatgggg acgactctcg ccgcatccgg caacacttga aaccatcgcg
     7261 gatggaaagc gccgagtgat cccgacgagt gttgcgccga ttactttttt tgtgcgcgaa
     7321 gacgcagagt ggatgattcc ccggcgcgaa gatgccgagg cgcttccctc cggactgaat
     7381 gcggacgcgt cggcggtttt gc
//
LOCUS       DASTPM010000317         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1020124, whole genome shotgun sequence.
ACCESSION   DASTPM010000317 DASTPM010000000
VERSION     DASTPM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020124"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1682
                     /gene="sufD"
                     /locus_tag="VFR42_05575"
     CDS             312..1682
                     /gene="sufD"
                     /locus_tag="VFR42_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU0368656.1"
                     /translation="MITATQAPEKYLEAFEHLKGRLGAQPHWLQSLRQEALARFAETG
                     FPTTHDEDWRFTNVAPVSNTQFELAGAENVTKQQLEPFAVTEFATRLVFINGLFAGKL
                     SSLAPMPAGVKVGSLAAQLKSDPASVEPHLGRYLNAQRDAFCSLNTAFIEDGTFVSVP
                     AKVVVDAPIYALFVTVPGASPAMNHPRNLIIAGEGSQVTVVEDYVSLGEGTTFSNAAT
                     ELVAGDSAHVSHYMVVREGEQAYNFSTLRIQQGRSANVATHSLLLSGALVRNNVHPVL
                     AGEGSECLINGLFMANGRQHMDNYMLVEHASPHCDSRQFYNGILNGQSHGVFHGRIIV
                     HKDAQKTDAKQTNRNLLLSDDAQIDTKPQLEIYADDVKCTHGATIGQFDDNALFYLRS
                     RGLDEASARHVLLLAFANECLDRMNSPQVRQHLEKLVVAGMPEAATFSGDRSPQVTER
                     NWEEVG"
     gene            1679..2941
                     /locus_tag="VFR42_05580"
     CDS             1679..2941
                     /locus_tag="VFR42_05580"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU0368657.1"
                     /translation="MSTVTKTPQPIHAAPAFDVHKVREDFPVLHQRVHGKPLVYLDNA
                     ATTQKPREVLDAIERYYRHDNSNIHRGVHTLSERATEAYENVRVAAQKFLNAADSREI
                     IFVRGTTEAINLVAQTYGRKNVGSGDEVLITAMEHHSNIVPWQLLCEEKGAKLRVAPI
                     NDRGELLLDEFEKLLSSKTKIVAVAHLSNALGTINPVREIVRLAHARNIPVLVDGAQA
                     VARMKVDVQALDCDFYALSSHKLYGPTGIGVLYGKTKLLEAMPPWQGGGDMIASVTFD
                     KTIYNRLPYKFEAGTPNIADTIGLGAAIEYVNRLGLQAIEEHEADLLAYATSAVEAIP
                     GVRVIGTAKEKAGVLSFVMEDIHPHDVGTILDSEGIAVRTGHHCAQPVMQRFNIPATA
                     RASFGLYNTREEVDALAAGIQKVREVLG"
BASE COUNT          682 a          892 c          871 g          597 t
ORIGIN      
        1 tgctcgacta catcgtgccc gactttgtcc acgtgctggt gaatggccgc atcgtgaagt
       61 caggcgggcc tgaactggcg ctggaactcg aagcaaaagg ctacagctgg accgagcacg
      121 agttggttgg aaagcgctaa ctgtcgcgtg gagcaggact ctggtcttgc gtcgagaaca
      181 gttattttct ggctttagcc gccgtggcaa ctccagcggt tgaaaccggc acgagacgca
      241 gcttggccgc aggccttaag gcctgcccca cccggtttca ggaaacagat tttcaagagg
      301 attgactaat cgtgattaca gcaacccagg ccccggaaaa atatctcgag gcattcgaac
      361 acttgaaggg ccggctcggc gcccagccgc actggctgca atcgctgcgg caggaagccc
      421 tggcccgttt cgccgaaacc ggcttcccca ccacgcatga cgaagactgg cgtttcacca
      481 acgtcgctcc cgtaagcaat actcagttcg aattggccgg cgcggagaac gtaaccaaac
      541 agcagctcga acctttcgct gtaacggaat ttgccacgcg gctggtcttt atcaatggcc
      601 tctttgccgg caaattatcc tctctcgcac cgatgccggc cggcgtcaag gtgggcagcc
      661 tggccgcgca gctcaagagt gatcctgcaa gcgtggagcc tcatcttggc cgctatttga
      721 acgctcaacg tgacgcgttc tgctctctca atacagcttt catcgaagac ggcacttttg
      781 tttctgttcc ggccaaagtt gttgtggacg cgcccatcta tgcgcttttt gtcactgttc
      841 ccggcgcttc gccggccatg aaccatcctc gcaacctgat catcgccggg gaaggcagcc
      901 aggttactgt cgtcgaagat tacgtttcac tgggcgaagg caccaccttt tccaacgccg
      961 ccacggagct tgttgccgga gacagcgccc atgtctcgca ttacatggtc gtgcgcgaag
     1021 gcgagcaagc ttataacttc tctactctgc gcatccagca gggacgcagc gccaatgtgg
     1081 ccacacactc actgctgctg agcggcgcgc tggtgcggaa caacgtccat cccgttttgg
     1141 caggtgaggg aagcgaatgc ctgatcaatg gcctgtttat ggccaacggc cgccagcaca
     1201 tggataacta catgctggtg gagcacgcca gcccgcattg cgacagccgg cagttctaca
     1261 acggcatctt gaacggacag tcgcatggcg tgtttcacgg tcgcatcatc gtccataaag
     1321 acgcgcagaa gactgacgcc aagcagacca accgcaacct gcttctttct gacgacgcgc
     1381 agattgacac caagccgcaa ctggaaatct atgctgacga tgtgaagtgc acccacggcg
     1441 ccaccatcgg ccagtttgac gacaatgcgc tcttttatct gcgttcgcgc ggcctggacg
     1501 aagcgtccgc ccgccacgtg ctgctgctgg catttgccaa tgaatgcctc gatcgcatga
     1561 attccccaca ggtgcggcag cacctggaaa agctggtagt cgccggcatg cctgaggctg
     1621 ccacgttctc cggtgatcgc tctccccagg tcacggagag gaattgggag gaagtgggat
     1681 gagcaccgta accaaaacgc cccagccaat tcacgccgcg ccggcattcg acgtgcacaa
     1741 ggtgcgcgaa gattttcccg tcctgcacca gagagtgcat ggaaaaccgc tggtgtatct
     1801 ggataacgcc gccaccacgc agaagccccg ggaagtcctg gacgcgatcg aacgctatta
     1861 tcgccacgat aattccaaca ttcatcgcgg cgtgcacacg ctctcggagc gcgcgactga
     1921 agcctacgag aatgtccgcg tagccgcgca gaaattcctg aacgcagccg acagcaggga
     1981 aatcatcttt gtccgcggca ccactgaagc cattaacctg gtggcgcaaa cctacggccg
     2041 aaaaaatgtc ggcagcggtg acgaagtcct gatcacagcc atggagcacc attccaatat
     2101 tgtgccgtgg caattgctgt gcgaagaaaa aggcgcaaag ctgcgcgtgg ccccgatcaa
     2161 cgatcgcggc gagctgttgc tggacgaatt tgaaaagctt ctgagcagca agacaaagat
     2221 tgttgcggtg gcacatctgt cgaacgccct gggcacgatc aatccggtaa gggagatcgt
     2281 gcgtctggcc catgcgagaa atattccggt gctggtggat ggcgcccagg ctgtggcgcg
     2341 catgaaggtg gatgtacagg cgctggattg cgatttctac gcgctctcca gccacaagct
     2401 ttacggccct accggcatcg gcgttctgta tggcaagact aagctcctgg aagccatgcc
     2461 gccgtggcag ggcggaggtg acatgatcgc ttccgtcact ttcgacaaaa caatttacaa
     2521 ccgcctgcct tacaaatttg aggccggcac gccgaacatt gcggacacca tcggcctcgg
     2581 cgcggccatc gagtatgtaa accgtctggg actccaggca atcgaggaac acgaagccga
     2641 tctgctggcc tacgccacgt cagcagttga ggcgattccc ggagtgcgcg tgatcggcac
     2701 tgcaaaggaa aaagccggag tgctctcttt cgtgatggaa gatattcatc cgcatgacgt
     2761 cggcaccatc ctggatagtg aaggcatcgc ggtgcgcacc ggacaccact gtgcgcagcc
     2821 ggtaatgcag cgtttcaata ttcctgccac cgcgcgcgcg tcgttcgggc tgtataacac
     2881 ccgtgaggaa gtggatgccc tggcggccgg aatacagaaa gtgcgcgagg tgttggggta
     2941 atgtccgaac tgcgcgactt atatcaggaa gtgattctgg agcacagcaa gaagccaagg
     3001 aattttcacg cgctggagaa cgccaatcac aaggcggagg ga
//
LOCUS       DASTPM010000318         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_40088, whole genome shotgun sequence.
ACCESSION   DASTPM010000318 DASTPM010000000
VERSION     DASTPM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40088"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1028
                     /locus_tag="VFR42_05585"
     CDS             231..1028
                     /locus_tag="VFR42_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0368658.1"
                     /translation="MTSAETPSASPEPAGDAGSREVGLHYDTRLPQLPWSRRIQIPLI
                     AALVYSVICVLGPTLRFEVLGWHHARGVHASGRRCVWAFWHRVIIPISWWARDMGIVV
                     MNTTAFDGQWTRKVIEWLGFGTAQGSSSRGGLRGLAVMAQRIEEGKDCAFTIDGPRGP
                     RYVAKPGPVMLARRTGCPITVFHIGVEYGKTFEKTWDHFLLPLPFSRAVVIFAPPIEV
                     PPNADANAMEDKHNEMQKELERVRDIAEAWFTLPESDRDRYRSEFGS"
     gene            complement(1037..2218)
                     /locus_tag="VFR42_05590"
     CDS             complement(1037..2218)
                     /locus_tag="VFR42_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0368659.1"
                     /translation="MGSGRILVLRPLAVIAILILVAYWAGARWGPRGPISVEAVPPVT
                     VDKGEVRNVSLAERDAALSNDEVLNVRVYRETSPAVANVLTKATEYDFWMDPVPVEGA
                     GSGFVIDGKGYILTNFHVVQGAQTIEVVLGDQSRYTAKFIGADQRNDVALLKVEPKGK
                     PLMALSLGDSAALQVGQKVLAIGNPFGFQSTLTTGVVSALGRTVQTSQTTFIDQAIQT
                     DAAINRGNSGGPLINSHGEVIGINSAIYTPSGTTAGIGFAIPINTAKRIAHDLITDGR
                     VHQAFLGVETIPINESFAEALGLPAQEGLLVQTTTRGGPAAEAGILGGDRVAQAGMRR
                     FYVGGDVLTAIDGQKIANRVDVDLMLNKKRPGEEVTITLFRGGKKMDLKVKLGERPQS
                     N"
BASE COUNT          464 a          846 c          581 g          519 t
ORIGIN      
        1 cacaaatgca gcgcttaaga gcagcgtccc gcgaagatag cggacgctgc ggcgacactc
       61 tcgtctttgg ggtttcaggc cttgcatact ttatctgtaa tcgcgacttg ccgctcttct
      121 ttatactata cggcatgcca actgtggcta cagcgaagag gattccaggg tcgaaacacc
      181 taccctggca catccatcct gaatgtcaca cagaatacca ttctgactaa atgaccagcg
      241 ccgaaactcc atccgcttcc cccgaacccg ctggcgacgc tggctcccgc gaagtcggcc
      301 tgcattacga cacgcgtctt ccgcagcttc cctggtcgcg ccgcatccag atccccctca
      361 tcgccgcgct ggtctattcc gtaatctgcg tgcttggccc gacgctccgt tttgaagtcc
      421 tcggatggca ccacgctcgc ggtgtccacg cttccggccg ccgctgcgtg tgggcctttt
      481 ggcatcgcgt catcattcct atttcctggt gggcgcgcga tatgggcatc gtcgtgatga
      541 acaccaccgc attcgacggc cagtggacgc gcaaagtaat cgagtggctc ggtttcggca
      601 ccgcacaagg cagttcgtcg cgcggcggcc tacgcggcct cgccgtcatg gcccaacgca
      661 tcgaggaagg gaaggactgc gccttcacca ttgatggccc gcgcggcccg cgctacgtcg
      721 ccaagcccgg tccagtcatg ctcgctcgcc gcaccggttg ccccatcacg gtctttcaca
      781 tcggcgtcga atacggcaaa acttttgaaa aaacctggga ccacttcctg cttccactcc
      841 cgttcagtcg tgccgtggtc attttcgcgc caccgattga ggtcccaccg aatgcggacg
      901 ccaatgcgat ggaagacaag cacaacgaaa tgcaaaagga actggaacgc gtgcgcgata
      961 tcgccgaagc ctggttcact ctccccgaat ccgaccgcga tcgctatcgc tccgaattcg
     1021 gctcgtaacc gcgcgattag ttgctttgcg gccgctcgcc cagcttcact ttcaaatcca
     1081 ttttcttgcc gccgcggaac aacgtaatgg tcacttcttc gcccggccgc ttcttattca
     1141 gcatcaggtc cacgtccacg cggttcgcga tcttctgtcc gtcgatcgcc gtcagcacat
     1201 cgccacccac gtaaaatcgc cgcattcccg cttgcgccac gcgatccccg ccgaggatac
     1261 cagcttccgc cgccggcccg ccgcgcgtcg ttgtctgtac cagcagtcct tcctgcgccg
     1321 gtagtcccaa tgcttccgcg aacgattcat tgatgggaat tgtttcgact cccaaaaatg
     1381 cctggtgtac gcgcccatcc gtaatcagat cgtgcgcaat ccgcttcgcc gtattgatcg
     1441 gaatcgcaaa cccgattccc gccgtcgttc ccgatggcgt ataaatcgcc gagttgatgc
     1501 cgatcacttc gccgtgtgaa ttgatcagcg gcccgcccga attccctcgg ttgattgccg
     1561 catccgtctg aatcgcctga tcgatgaagg tcgtctggct ggtctgcacc gtgcgcccca
     1621 gcgcgctcac cactcccgtc gtcaacgtgc tctgaaatcc gaacggattt ccgatcgcca
     1681 aaactttttg ccctacctgc aatgccgccg agtcgcccaa ggacaaagcc atcagcggtt
     1741 tgcccttggg ttccactttc agcagcgcca cgtcgttgcg ctgatccgca ccgatgaatt
     1801 tcgccgtata gcgcgattga tcgcccagca ccacctcgat cgtctgcgcc ccttgcacca
     1861 cgtgaaaatt cgtcaggatg taacccttgc cgtcaatcac aaagcccgag cctgctccct
     1921 ccaccggcac cggatccatc caaaaatcgt actcggtcgc cttcgtcagc acgttcgcca
     1981 ccgcgggcga agtctcccga tagacccgca cattcagcac ttcatcgttg ctcagcgccg
     2041 catcacgttc cgccaggcta acgttacgca cctcgccttt gtctaccgta acgggcggta
     2101 ccgcttccac gctgatcggt ccgcgcggtc cccagcgtgc ccctgcccag tacgccacca
     2161 gaatcaagat cgcaatcacg gccaatggcc gcagcaccaa aattctgcca gatcccatcg
     2221 gacttctccc tctcacaact aaactttgtt cttagccaga aatgataacc ccgatcaaaa
     2281 ctcgctgctc agttcgccga agcagccgcg cgcaatttgg ccgccagcgc atccacctga
     2341 ccccgcgtct cttctataga acccgagcaa tcgatttttt ctgcggccaa tgccaacttt
     2401 tcctgcaccg
//
LOCUS       DASTPM010000319         4165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_847408, whole genome shotgun sequence.
ACCESSION   DASTPM010000319 DASTPM010000000
VERSION     DASTPM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847408"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFR42_05595"
     CDS             complement(<1..259)
                     /locus_tag="VFR42_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0368660.1"
                     /translation="MSIAKSLLAEFEIQAPITRKFLERLPEDTLTWKPHEKSMTAGQL
                     AYHLASVPGGVIKFVQNNPAQAPDFASFQQPATRDEILKTFD"
     gene            complement(291..761)
                     /locus_tag="VFR42_05600"
     CDS             complement(291..761)
                     /locus_tag="VFR42_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0368661.1"
                     /translation="MTVATSVLEDLTLTVTQEIRVKASLETTFAALLEQLGPYNEKPD
                     GDPLPMVLEPWPGGRWYRDLGNGNGHFWANVQAIKKPTLLEFAGPLMMSHPVANNVQY
                     RLSEENGVTVIKFRHSGFGLIQPEFKTGVVTGWTYIHESVRKRAELGRSKSAVQ"
     gene            complement(758..1138)
                     /locus_tag="VFR42_05605"
     CDS             complement(758..1138)
                     /locus_tag="VFR42_05605"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0368662.1"
                     /translation="MYSHLMPRASTTSDAFNAVAEPRRRDILSFLALDERSVGDIVDA
                     LSLEQPSVSKHLRVLKDVGLVHVRRDGRHMLYRTNADAIRPLHEWTETFERFWRHQLT
                     RIKERAEAKSLPISNSQKTEKEPS"
     gene            complement(1263..1886)
                     /locus_tag="VFR42_05610"
     CDS             complement(1263..1886)
                     /locus_tag="VFR42_05610"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU0368663.1"
                     /translation="MKVGIIIVVVLLILVLILGSSFVSRRNQMAVKREAVNAAWSQVD
                     VVLQRRADLIPNLVATVKGFAVHEEQVFSEIAQARSALIGARTPADKIAANGALDSAL
                     SRLLVITENYPQLKSNENFLRLQDELAGTENRIAVERRRYNDVVQEYNTFISLFPNSL
                     VASMSGFTRNNAYFKADEGSRTAPKVNFDFNKSQAPAATPTPAPAKP"
     gene            complement(2130..2879)
                     /locus_tag="VFR42_05615"
     CDS             complement(2130..2879)
                     /locus_tag="VFR42_05615"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEU0368664.1"
                     /translation="MIAREEKLAEFTRRLHDAAGDNLHSIVLYGSAARGEYHEQKSDL
                     NVLCVLNSAKANDLARLARAIHWWSGQLKEPPPQFFTLEELRHCADVFSIELLDIGQN
                     HKILFGPDPIAGLEVPMNLHRIEIEHDLRALLQKLRVHFVHYSENEAQLREIYAKSIS
                     GMTVLLRHVLIALGEDVPSDRSHLYQRIAELTGADAAVFELGRALRDNHPSAEIPRAF
                     GKYLEALTAVIHALDAIVPKREWQRLKKAHP"
     gene            complement(3026..3874)
                     /locus_tag="VFR42_05620"
     CDS             complement(3026..3874)
                     /locus_tag="VFR42_05620"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU0368665.1"
                     /translation="MTSTHRARLLRLPVYALPLLFALTAAFADDIKRIAPTGYVTDLA
                     GVIPASTRQRLEALCTELEQKTGAQLAIVTVRSLDDRPVEDYAVDLFKHLGVGSKKQD
                     SGVLLLLAPNDRKYRVEVGYGLEPIINDARAGDAGRAMVPLLRQNDFGAAAEVAAWQL
                     AKYIADDKGVTLSGAPPARPVSRQTHSEKSPISLFAVFFILFFLFQIFGSAVRRTSGR
                     RGGGMDGCWWILPFLGGWGGGGRGGWGGGGSWGGGGSWGGGGSGGGFGGFGGGSSGGG
                     GASGNW"
     gene            complement(3888..>4165)
                     /locus_tag="VFR42_05625"
     CDS             complement(3888..>4165)
                     /locus_tag="VFR42_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368666.1"
                     /translation="LPPCEDRKMGQRSSHTSILGFVAWMRTSFPQVFSCRHLFPRTVR
                     PSVLNCVLSAIRFCIGKPALSSRLALALFFKNRDAYPSPTAISVTMR"
BASE COUNT          911 a         1001 c         1432 g          821 t
ORIGIN      
        1 cgtcgaaagt cttgagaatt tcgtcacgcg tggccggttg ctggaaagag gcaaaatctg
       61 gagcttgagc tggattgttc tgcacgaatt taatgacgcc gccgggaacg gacgcgaggt
      121 gataagcgag ctggccggcg gtcatggact tttcgtgtgg cttccaagtc agtgtgtctt
      181 cgggcaggcg ttcgaggaat ttgcgcgtga tgggcgcctg gatttcgaac tccgccagca
      241 gtgattttgc gatcgacata tgattctcct tttgatgtgg aaacggaaat ctactggacg
      301 gccgacttag agcggcccag ctcggcgcgc ttgcggacac tttcgtgaat gtaggtccag
      361 ccggtgacga cgccggtttt gaattccggc tggatgagtc cgaatccgga gtgacggaac
      421 ttgatcacgg tgacaccgtt ttcttcgctg agacggtatt ggacgttgtt ggcgacagga
      481 tgggacatca tgagcgggcc ggcaaattca agaagcgttg gcttcttgat ggcttgaacg
      541 ttggcccaga aatggccatt gccattgccg agatcacggt accagcggcc gccaggccag
      601 ggttcgagaa ccatggggag aggatcgccg tcgggctttt cgttgtaggg gccgagctgc
      661 tcaaggagcg ccgcgaaggt ggtctcgagc gatgccttga cgcggatttc ctgggttacg
      721 gtcagggtta gatcttccag tacagatgtg gctacagtca tgacggctcc ttttcggttt
      781 tttgggaatt ggaaatggga agcgatttgg cttcggcgcg ctctttgatg cgggtgagct
      841 ggtggcgcca gaagcgctca aaggtttcgg tccactcgtg gagggggcga atggcatcgg
      901 cattggtgcg gtagagcatg tggcggccat cgcggcgaac gtggaccaga ccgacgtctt
      961 tgaggacgcg gaggtgtttg gaaacggagg gctgttcgag gctgagagca tccacgatat
     1021 caccgaccga gcgttcgtcg agagcgagaa aggagaggat gtcgcggcgg cgcggttctg
     1081 cgacggcgtt gaaggcgtcg gaggtggtgg aggcgcgcgg catgaggtga gaatacattc
     1141 ccatatggga atatgtcaag cgaaaaatct gatgtcttca agaccatgga aatcagcaac
     1201 ttacagggca gaagagagac tggcggggcc ggagctagga ttcgggcccc acgcgagaga
     1261 ggtcagggct tggcaggagc tggagtcggc gtcgccgcgg gagcttgcga tttgttgaag
     1321 tcgaaattca ccttaggcgc ggtgcgggag ccttcatcag ctttgaagta ggcgttgttg
     1381 cgggtgaagc cgctcatgct ggcgaccagg ctgttgggga agagcgagat gaaagtgttg
     1441 tactcctgaa cgacgtcatt gtaacggcgg cgctcgacag cgatgcggtt ttcggtgccg
     1501 gcgagctcgt cctgcaggcg gagaaaattc tcattggatt tgagctgggg atagttttcc
     1561 gtgatgacca gcaaacggct tagagccgaa tcaagcgcgc cgttcgcggc gattttgtca
     1621 gcgggagttc ttgcgccgat caaagcagag cgagcttggg cgatttcact gaagacctgt
     1681 tcttcgtgga ctgcgaaacc ttttacggtg gcgactagat tagggatgag atcagcgcgg
     1741 cgctgcagca cgacgtcgac ttgggaccat gcggcgttga cggcttcgcg cttgacggcc
     1801 atctggttgc gacggctgac gaacgagctg ccgaggatga gcacgagaat gagcaggact
     1861 acgacgatga tgatgccaac tttcattggg actctccttt acgtaacgga tctttacgcc
     1921 taacgctagc gaatgcgccg gaggcaaaca gaaggcggat cgcagaggga ggtcgcgcac
     1981 tgacggaaaa ggcgggcgcc tcagaaggcg gccgttagca gggatgcggg acgagaaaaa
     2041 gcacaggtct gaagacccgc cactacgaac gcggggcaac gctcgcggtg aaatcgaaaa
     2101 tgtaagggag tgaatcatca tttcgtccat tacggatgcg ctttcttcag gcgctgccat
     2161 tcgcgtttgg ggacgatggc atcgagcgcg tggatgacgg cggtgagggc ttcgagatat
     2221 ttgccgaagg cgcggggaat ttcggccgag ggatgattgt cacgcagagc gcggccgagt
     2281 tcgaagacgg cggcgtcggc gccggtgagc tccgcgatgc gctgatagag atggctgcgg
     2341 tcggaaggaa cgtcttcacc gagtgcgatg aggacgtggc gaaggagaac cgtcatgccg
     2401 gagatggact tggcgtaaat ttcgcggagc tgcgcttcgt tttcggaata gtggacgaaa
     2461 tggacgcgga gtttttgcag cagagcgcgg agatcgtgtt cgatttcgat gcgatggaga
     2521 ttcatgggga cttcgaggcc ggcgatggga tcggggccaa agaggatttt gtggttctgg
     2581 ccgatgtcga gcaattcaat ggagaagacg tcggcgcaat ggcgaagttc ttcgagagtg
     2641 aagaattgtg gaggaggctc tttgagctgg ccggaccacc agtggatagc gcgggcgagg
     2701 cgagcgagat cgttggcctt ggcggaattg aggacgcaga ggacgttcag atcggacttt
     2761 tgttcgtgat attcgccgcg cgcggcagag ccgtagagga cgatggagtg gagattgtcg
     2821 ccggcagcat cgtggaggcg gcgggtgaac tcggctagtt tttcttcgcg ggcaatcatg
     2881 tagtcctcaa agcgaacaga aggtttgctg cgacccaatt tagtgcttaa agccgaatca
     2941 attcctcagg gggctgaagc ctgattggca gccaagctcg gatgtcgggg ttaaagctcc
     3001 gaccccaaat gccctatgcc cgacactacc agtttccgga agcgccgcca ccgccactgg
     3061 agccgccgcc gaatccgccg aagccaccac cactgccgcc gccgccccag cttccaccac
     3121 caccccaact gccgccaccg ccccaaccgc cacgacctcc gccaccccag ccgccaagga
     3181 aaggaaggat ccaccaacag ccgtccatgc cgccgccgcg acggccggaa gtccgccgga
     3241 ctgcggagcc gaaaatctgg aacaggaaga agagaatgaa gaaaacagcg aagaggctga
     3301 tgggcgattt ttcggagtgc gtctgacgcg aaacaggtcg agcgggcggc gcgccggaga
     3361 gagtgacgcc tttgtcgtcg gcaatgtatt tggccaattg ccaggcggcg acttctgcgg
     3421 cggcgccgaa atcgttttgc cgaaggagag gaaccatcgc gcgtccggcg tcgccggccc
     3481 gagcgtcatt gatgatgggc tcgagaccgt aaccgacttc gacacggtac ttgcggtcgt
     3541 tgggagcgag gagtaagagg acacctgagt cctgtttctt tgacccgacg cccagatgtt
     3601 tgaagaggtc gacagcgtaa tcttcgacgg gacgatcatc gagagagcga acggtgacga
     3661 tggcgagttg ggctccggtt ttttgttcaa gttcggtgca aagagcttcc agccgctggc
     3721 gggtgctggc ggggattacg ccggcaagat cggtgacgta cccggtgggg gcgattctct
     3781 tgatgtcgtc cgcgaacgcg gcagtgagcg caaacagaag cggaagtgca tagacgggaa
     3841 gccgcagcag gcgtgcacgg tgtgtggagg tcaaaatcga ttccagatca gcgcatcgtt
     3901 acggatatgg cggtgggcga cggataagca tcgcggtttt tgaaaaaaag agcaagggcg
     3961 aggcggctgg agagagcagg tttgccgatg caaaaacgga tagcggaaag cacgcaattg
     4021 agtacagacg ggcgaactgt tcgtgggaac aggtgacgac aagagaaaac ctgcggaaag
     4081 gaagtgcgca tccaagcaac gaaacctaga atggaagtgt gagaagaccg ttgacccatc
     4141 tttctatctt cacagggcgg caacc
//
LOCUS       DASTPM010000320         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_270849, whole genome shotgun sequence.
ACCESSION   DASTPM010000320 DASTPM010000000
VERSION     DASTPM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270849"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VFR42_05630"
     CDS             complement(<1..225)
                     /locus_tag="VFR42_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368667.1"
                     /translation="MRAHDVRPRRGSGRIVAAYGVFAWLTLYLAFAGNAKAQEADLAA
                     HEWGTFTSIAGNAGTAVQWFPWAVPSDLPKF"
     gene            complement(222..923)
                     /locus_tag="VFR42_05635"
     CDS             complement(222..923)
                     /locus_tag="VFR42_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368668.1"
                     /translation="METRSVRAFRIALTAFVAMFAALSPAFAGPPLICHAFDIGNAKS
                     LPWVSHDWNLNGGESYDTHDLAKDTIAILDASQVTLVHMETLRRATLYARKDAQAAKQ
                     LLLMVTARAEATQNSGHPDALAVFDAGYLAEAYKQWLGEGGQNPANGMDGYGLVKKAL
                     GFRGNDGQMEFAAALVTLSGPARENQEHAQKARDGAKTDALLARNLNTRFLGAGSQTM
                     AEMMQRNATTKVARE"
     gene            complement(986..2062)
                     /locus_tag="VFR42_05640"
     CDS             complement(986..2062)
                     /locus_tag="VFR42_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEU0368669.1"
                     /translation="MKSDYLWDGSGEPDPELQKLERALGQFRSQAEAPTYPVDVEAGK
                     QRGSKVIGLAWRIDRLAASVVFGVAAATVAVVAFLDVPKSPTGPGWDVARLEGAPSVG
                     ELTVAKTGGMAKLHVGQVLTTNATSRASVQVANIGELYVDPGSRVRLLETGSARKRIA
                     VEVGTIHAAIWAPPGEFVVDTPSATAVDLGCAYTLQVAADGSGTLRTKMGWVGFHQNG
                     HDSFIPAGAMCLTRAKEGPGTPFFEDSPEEFRLALHALDFGGLSAAERSEALQTVLRK
                     ARARDGFTLWHLLARVSDEERPIVYDRLTALVRAPSGTTREGVLRLDRPMMDAWWNAF
                     DLGDIGVWRFWEQREAPRVKKAAG"
     gene            complement(2121..2729)
                     /locus_tag="VFR42_05645"
     CDS             complement(2121..2729)
                     /locus_tag="VFR42_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0368670.1"
                     /translation="MNDGMAATGWMQIRGASLTSVSDPARADTASDEALVCAAQAGDR
                     VAFGTLYQRYSRMVHGILLARVPRAAAEDLLQDVFLQALPRLSLLRDATRFGGWLAAI
                     ARNRASDYHRQTRPTQEIEENFLENSKDGGRNSTKQVSEGLFFLEAVRSLPEAYREPL
                     ILRFVEGMTGPEIAERTGLKHGSVRVNLYRGMQMLREKLEKK"
     gene            2861..>3294
                     /locus_tag="VFR42_05650"
     CDS             2861..>3294
                     /locus_tag="VFR42_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0368671.1"
                     /translation="MAPPALEFRPHPLLKNGHVMTVASAFWRRSFSLPSPEDRLFPVD
                     PESRILGHCHWQQGKDKATPLLVLVHGLEGSSDSNYMRGIAEKASQRGFHVIRLNQRN
                     CGGTELLTPTLYNSGMSADYRAVLEELAGDGFAKIFFAGYSMG"
BASE COUNT          638 a         1176 c          784 g          696 t
ORIGIN      
        1 gaatttcggc aggtccgaag gaaccgccca cgggaaccat tgcaccgctg tcccggcgtt
       61 acctgcaatg gatgtaaacg tgccccactc gtgcgccgcc agatcggcct cctgtgcctt
      121 ggcatttcct gcgaacgcca gatagagggt caaccaggcg aatactccgt atgcagcgac
      181 gatgcggcca ctgccacgtc tcggtctgac gtcatgggct ctcattcccg tgccaccttt
      241 gttgtcgcat tccgctgcat catttctgcc atcgtctgac tccccgcgcc gaggaatcgc
      301 gtattcagat tccgcgccag cagcgcatct gttttcgctc cgtcccttgc tttctgtgcg
      361 tgttcctgat tttcccgtgc cggcccggac aacgtaacga gcgccgctgc aaattccatc
      421 tgtccatcat taccgcggaa ccccagagcc ttcttgacca gcccgtatcc gtccatcccg
      481 ttcgccggat tttgtccgcc ctctcccagc cactgtttgt acgcctcggc gagatagccc
      541 gcatcaaaca ccgccagtgc gtccggatgc cccgagttct gcgtcgcctc ggctcgcgcg
      601 gtcaccatca gcaacagctg ctttgcggct tgtgcatcct tccgcgcata cagcgtcgcc
      661 cgccgcagcg tctccatatg gaccagcgtc acctggcttg catcgagaat cgcaatcgtg
      721 tcctttgcca ggtcatgggt gtcgtagctc tccccgccgt tcagattcca gtcatgactc
      781 acccacggca gcgatttcgc attgccaata tcaaatgcat ggcaaatcaa tggcggcccg
      841 gcgaaggccg gactcaacgc agcgaacatt gcaacgaatg cagtcaaggc gattcgaaac
      901 gctcgaactg accttgtttc catgtttgtc tccttcggcg ctctgggcag cggcaactaa
      961 ggagacgtct gccactcgac cattgttaac cggctgcctt cttcactcgc ggcgcttcac
     1021 gctgctccca aaatctccac acaccgatat ctcctaaatc aaacgcgttc caccaggcgt
     1081 ccatcattgg tcgatcgaga cgcagaacgc cctccctcgt cgttcccgaa ggcgcccgca
     1141 ccagtgccgt tagtcggtca tagacgattg gcctttcttc atcgctcacc cgcgccagca
     1201 gatgccacag cgtgaatcca tctcgcgccc gcgcttttcg caataccgtt tgcaatgcct
     1261 cacttctctc ggccgcgctc aatcctccaa aatccagtgc gtgcagcgcc aagcgaaact
     1321 cctccggcga atcttcaaaa aacggagtgc ccggtccttc cttcgctcgc gtcaggcaca
     1381 tcgctcccgc cggaataaac gagtcatggc cgttctgatg aaatcccacc cagcccatct
     1441 tggttcgaag cgtacctgat ccgtccgccg ccacctgcaa cgtgtacgcg caacccaaat
     1501 ccaccgccgt cgccgacggc gtgtccacca caaattcccc cggcggagcc cagatcgcgg
     1561 cgtgaatcgt gcccacctcc accgcaattc gcttgcgagc tgaacccgtc tccagtaatc
     1621 gcacccgcga tcccggatcc acgtacaact ccccgatgtt cgccacctgt accgacgccc
     1681 gcgacgtcgc gttcgtagtc aagacttggc ccacgtgcag tttcgccatc ccgcccgtct
     1741 tcgcgacggt caactcgccc accgacggcg ccccttccag ccgcgcaaca tcccaaccag
     1801 gtccagttgg cgatttcgga acgtcgagaa acgccaccac ggcaaccgtg gcagccgcca
     1861 cgccgaacac cactgacgcg gccaatcgat ctatccgcca cgccaaacca atcaccttcg
     1921 aacctcgctg tttccccgct tcaacgtcca caggatacgt cggcgcttcg gcctggctgc
     1981 gaaactgtcc cagcgccctc tccagctttt gcaattccgg atcgggctcg cccgaaccgt
     2041 cccacaaata atcgctcttc atttcttcgc ctcgaaattc aaatcaatcg ctctccgaac
     2101 cgcgccctcg cctttgcgca tcacttcttc tccagcttct cgcgcaacat ctgcatcccg
     2161 cgatacaggt tcacgcggac cgagccatgc tttagtccgg tcctttcggc gatctccggt
     2221 ccagtcatgc cctccacaaa ccgcaaaata agcggctcac gataggcttc cggcaatgac
     2281 cgcaccgcct ccagaaaaaa caatccttcc gacacctgtt tggtcgagtt tcgcccgccg
     2341 tcttttgagt tctccagaaa attctcttcg atctcttggg tagggcgcgt ttgccggtga
     2401 tagtcgctgg cacgatttcg cgcgatggca gccagccaac cgccaaaccg cgtcgcatcg
     2461 cgcagcaaag acagcctggg cagagcctgc agaaatacgt cttgcaacaa gtcctcagcc
     2521 gccgctcgtg gcacgcgcgc cagcaaaatt ccatgcacca ttcgcgaata tcgctggtag
     2581 agcgtcccaa atgccacgcg gtcgcccgcc tgcgcggcgc acaccagtgc ctcgtcagaa
     2641 gcggtgtccg ctcgtgccgg atcgctcaca ctcgtcaggc tcgctcctcg aatttgcatc
     2701 catcccgtgg cggccatgcc atcgttcacg cctctcgcac ccagttctcc ctgcctacag
     2761 tgacgcacgg ctcatccggt ttgttaacca tacgtccggg caaatcaaat cgaaagtctc
     2821 ccgtgttgcc ggttcgcctc gcctcgctta cattggaaca atggccccgc ccgcgctgga
     2881 atttcgtcca cacccgctcc tcaagaacgg tcacgtcatg accgtagcct ccgcattctg
     2941 gaggcggagt ttcagcctgc cttctcccga agaccgtctc tttccagtcg atccagaatc
     3001 cagaatcctc ggccattgtc attggcagca gggaaaagac aaggccactc ctctattggt
     3061 actggtacat ggtttagagg gttccagcga ttcgaactat atgcgcggta tcgcggaaaa
     3121 ggcctcacag cgcggcttcc acgtgatccg cctcaaccag cgcaattgcg gaggcaccga
     3181 actcctcacc ccgactctct acaattccgg aatgagcgcg gattaccgtg ccgttctcga
     3241 ggaactcgcg ggtgacggtt tcgcgaagat attcttcgcg ggatattcca tggg
//
LOCUS       DASTPM010000321         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_645403, whole genome shotgun sequence.
ACCESSION   DASTPM010000321 DASTPM010000000
VERSION     DASTPM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645403"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2765
                     /locus_tag="VFR42_05655"
     CDS             <1..2765
                     /locus_tag="VFR42_05655"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU0368672.1"
                     /translation="TDWTAPDTVAPGHIPVTANWKFTYKQDPEVLTLESALFETPQSR
                     IQASGTLGHSDSVLELHVRADELEAFNDLIQSIEEVVPGTPEEVVPRIHGAVNWDGKM
                     SGPLGGPTFTGHVRGERLAYGKFHADSVESDVTYSPSELSVSRGHVQYGAMRADFQGS
                     LDLDRWGFRAENEWTAEASVEKIPVASMLAVVGEKYPVEGTLTGQFHGRGTRQEPTIT
                     GLFDLADGKVYGFEFNRLRGQVNVQPDEARISNAELRVFAPGKETGGAGIITGSVGYQ
                     FSTKNVSAELVGAGLPLRNIEKIQSERLPISGQISFHLKASGPYQAPVGDGSMRVVDF
                     RVGQEVIGSFDGELHSDGKIAKLDLHSAMSDGGISGGYTLGLTEPYPVSGKITLQNIA
                     LDPFLLTALHMEHISGHGTADGEVSLEGNLKQPESLIVDANFSRLLMNYANVQLENTG
                     PIHFRSSKDEITIDSATFKGTDTNLQLAGNVKFSGRRNLNLNLNGALDLRLLSGFVPG
                     LDARGPAQVNAAFEGTLDRPRITGKVHIENAQARAIDFPTGLSGIKGDLIFDASRLYF
                     ENVTAQVGGGELSASGSVYYADRPIRFDITTRTDRVRIRYPEGMSWQAAGNLRLTGTP
                     QGGLISGRVQIERVNMSGGLETAGNIMGGGESGSAVSSSPFLRNLQFDIEAVSTPDAR
                     MEWPSAELEAEANLRVRGTWDHPILLGHIHVLSGDLYFHGSRYRVARGDLNFANPFQL
                     NPDINVEATTTIQQYEITLNFSGPANKLSLSYRSDPPLPSNDIITLLALGQTSSESEV
                     RSGGTGAGAVSAGSGAGALLSEAISSQVGGRLERLFGITRLRVDPGLAGVGSTGSEQN
                     AAARITVEQQIARDLTITYVSNVSSTQQQVIQVEYNVNRNVSIVALRDYNGTFGIDVK
                     IKKRFD"
     gene            2980..>3468
                     /locus_tag="VFR42_05660"
     CDS             2980..>3468
                     /locus_tag="VFR42_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368673.1"
                     /translation="MLWRVCGVVVLAATSVAGRTLGQEQHSHSSADRLGEVWFPVSCL
                     PEVQKTFERGVALLHSFAYAAAEKQFAEVSAADPKCAMAHWGVAMSLFHQIWERPEDS
                     TLKRGPEEMEQAQKIGAKTERERGFISALAAFYSDPSKDHYQKQATAYSDAMAKVYQQ
                     NPN"
BASE COUNT          768 a          978 c         1062 g          660 t
ORIGIN      
        1 aaaccgactg gacggctccc gatacagtcg cacccggtca cattccagtc acggcgaact
       61 ggaagttcac ttacaaacaa gacccggaag ttctgaccct cgaatccgcc ctgtttgaaa
      121 ctccgcaaag ccgcatccag gccagcggca cgctcggcca cagcgactct gtccttgaac
      181 tgcacgtgcg cgccgacgag ctcgaagcgt tcaacgactt gatccagtcc attgaagagg
      241 ttgttcccgg cacgccggaa gaagtggttc cgcgcattca cggcgccgtc aattgggatg
      301 gcaagatgag cggtccgctc ggcggcccca cgttcaccgg ccatgtgcgc ggcgaacgcc
      361 tcgcttacgg aaaattccat gcagactcgg tcgaaagcga cgtcacctat tcgccttcgg
      421 aactttccgt ttcacgcggg cacgtgcaat acggcgcgat gcgcgccgac ttccaaggaa
      481 gtctggacct cgataggtgg ggtttccgcg cggaaaatga atggaccgcc gaagccagcg
      541 tcgaaaaaat tcctgtggcc agcatgctcg cggtggtcgg cgagaaatat cccgtcgagg
      601 gaactctgac gggacaattt cacggccgcg gcacgcgcca ggaaccgacg ataaccggcc
      661 tcttcgattt ggcggacgga aaagtttacg gctttgaatt taatcgcttg cgcggccagg
      721 tgaatgtgca gcccgacgaa gcgcgcatca gcaatgccga gctgcgcgtt ttcgcgccgg
      781 gaaaagaaac cggcggagcg ggcatcatca ccggcagcgt gggctatcag ttctccacaa
      841 aaaatgtttc cgcggaactg gtcggtgcag gcctgccgct tcgcaatatc gaaaaaattc
      901 aatccgagcg cctgccgatt tccggccaaa tctcctttca cttgaaagcg tcggggccct
      961 atcaggcgcc ggtcggcgac ggctccatgc gcgttgtgga tttccgcgtc ggacaggaag
     1021 taatcggcag cttcgacggc gaactgcatt ccgacggaaa aatcgccaag ctcgatttgc
     1081 attccgccat gagcgacggc ggcatttccg gcggctacac gctgggcttg accgagccct
     1141 atccggtcag cggcaaaatc acgctgcaaa atatcgcgct cgatccattc ttgttgacgg
     1201 cgttgcacat ggaacacatc agcggccacg gcacggccga cggcgaagtg tcgctcgaag
     1261 gaaatctgaa acagccggaa agcttgattg tggatgcgaa cttttcgcgg ctgctgatga
     1321 attacgccaa cgtacagctg gaaaataccg gacccattca tttccgttcg tcgaaggatg
     1381 aaatcacgat tgattcggcg accttcaaag gcaccgacac caacctgcag ctagccggca
     1441 acgtaaagtt ttctggacgc cgcaatctca atctgaattt aaacggcgcc ctggatttgc
     1501 gattgctcag cggttttgtg ccggggctcg atgctcgcgg cccggcgcaa gtgaatgccg
     1561 cgtttgaagg aaccttggac cggccgcgca tcaccggcaa agtgcacatt gaaaacgcgc
     1621 aagcgcgcgc gatcgatttt ccgaccggac tgagcggcat taagggcgac ctgatttttg
     1681 acgcttcgcg cctgtatttt gaaaatgtta ctgcgcaagt cggcggcggc gagttgagcg
     1741 ccagcggcag cgtgtactac gcggaccgcc cgattcgttt tgacatcacc acgcgtaccg
     1801 accgtgtgcg catccgctat cccgaaggaa tgagctggca ggccgcggga aatctgcgcc
     1861 tgaccggcac gccacaaggc gggttgattt ccggccgcgt gcagatcgag cgcgtgaaca
     1921 tgagcggcgg tctcgaaacc gccggcaaca ttatgggcgg cggcgaaagc ggcagcgcag
     1981 tgagcagttc gccgtttctg cgcaacctgc agtttgacat cgaggcggtg tcgacgcccg
     2041 acgcgcggat ggaatggccg agcgcggagc tggaagccga agcgaatctt cgcgtgcgcg
     2101 gaacctggga ccatccgatc ttgctgggac acatacacgt gctttccggc gatctgtatt
     2161 tccacggcag ccgctatcgc gtggcgcgcg gcgatttgaa ttttgcgaat ccgttccagt
     2221 taaatccgga catcaacgta gaagcgacga cgacgattca gcagtatgaa atcacgctga
     2281 acttcagcgg gccagcgaac aaactttctc tgtcctatcg ctccgacccg ccgctgccga
     2341 gcaacgacat catcactctg ttggcactgg ggcaaaccag ctcggaatcg gaagtgcgca
     2401 gcggagggac cggagcggga gcggtaagcg cgggaagcgg cgcgggcgcg ctgttgtccg
     2461 aagcgatttc gagccaagtg ggcgggcgac tggaaagact gtttggcatc actcgcctcc
     2521 gagtggatcc gggacttgcg ggagtcggtt cgaccggatc ggaacagaac gccgcggcac
     2581 gcattacggt ggagcagcag atcgcgcgcg acctgacgat tacctacgtg agcaacgtga
     2641 gttcaacgca gcagcaggta atccaagtgg agtacaacgt gaaccggaac gtgtcgatcg
     2701 tggcgctgag ggattacaac ggaacctttg gaatcgacgt gaagatcaag aagcgctttg
     2761 attgaaggtc gcgttccatt ttggaacgac gacgggaagg tagccccacc ccctgttttt
     2821 tctgtagcta acggatctaa agggcttagc cgcagcgtcg acctctccgc ctgcagccct
     2881 cgcgtcaaac tgcgggtcat ccggagattc ggtttgtctc acaatggaac gagtgaggaa
     2941 gcagcctaag gcgagagtga accggcttgg aggtggcaga tgttatggcg agtgtgtggc
     3001 gtagtggttc ttgcggcgac gagcgttgcg ggaaggaccc tggggcagga gcagcattcg
     3061 cattccagcg cggatcgttt gggcgaagtt tggtttccag tttcgtgctt gccggaggta
     3121 cagaaaacat tcgaacgcgg agtggctctg ctgcactcgt ttgcttacgc cgcagcggag
     3181 aagcagtttg ctgaggtcag cgcagcggac ccaaagtgcg ccatggcgca ttggggcgtt
     3241 gccatgagcc tgttccatca gatctgggag cggccggagg actcgacgct gaagcgcggg
     3301 cccgaggaga tggaacaagc acaaaaaatt ggggcaaaga cggagcgtga gcgcggattt
     3361 atctcggcac tagccgcctt ctacagcgat ccttccaaag accattacca gaagcaagcc
     3421 acagcctatt ccgacgcgat ggcaaaggtt tatcagcaga atccgaat
//
LOCUS       DASTPM010000322         3967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1395094, whole genome shotgun sequence.
ACCESSION   DASTPM010000322 DASTPM010000000
VERSION     DASTPM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395094"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2859
                     /locus_tag="VFR42_05665"
     CDS             <1..2859
                     /locus_tag="VFR42_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU0368674.1"
                     /translation="GHVRTAAQFETTWKELRSALDEAGDAGLLLGASGAQILLDGVPL
                     GAAAAERSFAQLLSTSGIASIHFAAHLSQAQFARFVRAFPSGNAKPQSLAEQLKQALA
                     GETTIKVNEIRYVAEDSSIAGAKIAANLTAKVLGAQGDKFKDFLEDPNRLLQMILAAE
                     GSRGGSGGGPGGGGPGFGGGGGVPGGSGGSGGGSGAGAGTAGLPGGNLWESGTGGGSG
                     GSGSAGGGGAANGFVPSGTAGKGGNSFPALSTAAAALGAAAPANPSKWLTASALLHSG
                     SAGGAAEFTVQEEEIRSMLSLFAQLGRARKDAGSGGMDATTFQSRLSAMPVRNQVTLQ
                     QALAGLAAQAPTEKPDQPMLLKLAEHVAIRFALDSYEKGELRVNAVKQLLDRMNTEIE
                     ALRKILTSQEEQMASAGLNVQSYTELLDQEFWEQVPDENKKEVLASDEAWCVPPRNVR
                     GFLEEMLRRGELKTVNEILMKYAACIGLEPEEARRTTAIGLGELAELYGSGDGSALIE
                     SIRRVGNQLAAERDPELQTLISAAFVRLSQEAGSKRCYPAMQQALASLDSVEAQRPGA
                     SQNLRPRIGAEERIPEFVEETLRTGEIADGLLDILLLMPKATINYITNRFGHSGFRDD
                     CELLADLVKGLGEDAVHRLAETLQTASANEAAEVVGLLGQLDPDSVARVLPVRLSQWP
                     RSVHDRVIRQLCTVPQESRGILLLSIFDALDSLIKPLALDEIGMTGLPDAIHALLDLA
                     SSDGIGGYIRLKAIEALGRLRATAAAPSLQTILEARQVWRWQYPAELRVAAAQALQRI
                     DPVTTMQRLEAWGFEKKDLALEPTDPEANSAVIRQRRYARLKLNRNLAFITTNLRENF
                     RLNVVELNLGGGIGSGERHLAPGTLVHFKLSQGVRTIKAQALVRGARPQAMAFEFVDM
                     DLEDRARLRKILLELGGLPQAAHVASRARRRGRMAVLK"
     gene            3011..>3967
                     /locus_tag="VFR42_05670"
     CDS             3011..>3967
                     /locus_tag="VFR42_05670"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368675.1"
                     /translation="MDAAKILDRVVASFIGTKHERDIKKLQPVVAAISAKEPEMQLLS
                     DDALKSEFAKLKDEVQARLKDVDASEPGYREKMQAALEPAIVPSFALAREAGRRFLNM
                     RHFDVQLIGGLVLHDGKIAEMKTGEGKTLVATLPAVLNSLASRGVHIVTVNDYLARRD
                     AEWMSPLYRGLGLTIGVIVHDLDDAQRRAAYGADITYGTNNEYGFDYLRDNMKYDITH
                     CVQRGHQFAIVDEVDSILIDEARTPLIISGPAEESTDKYYRVDKIIPKLIKEIDYTID
                     EKHRTATLTEEGVSKTERLMGLGNLYDPAHMEIIHHVYQALRA"
BASE COUNT          796 a         1322 c         1106 g          743 t
ORIGIN      
        1 gggcacgtgc gtacggcggc tcaatttgaa acaacttgga aggaactgcg ctccgctctc
       61 gatgaggccg gcgacgccgg gctcctgctt ggtgcatccg gcgcgcaaat tctcttagat
      121 ggcgtgccgc ttggcgcggc agccgccgaa cgtagttttg cccagctgct ttccacttcc
      181 ggcatcgcca gcatccactt cgccgcccat ctctcgcagg cccagtttgc ccgcttcgtg
      241 cgcgctttcc ccagcggaaa cgctaagccg cagtcgctcg ccgagcaact caagcaagct
      301 ctcgccggcg aaaccaccat caaggtgaac gaaattcgct acgtggccga agactcttcc
      361 atagcaggcg caaaaatcgc cgccaatctc accgccaaag ttctcggcgc acagggcgac
      421 aagttcaagg attttctgga agatccgaat cgtttgctac agatgattct cgccgcggaa
      481 ggttcgcgcg gaggcagcgg aggcggccct ggcggaggcg gtcccggatt tggcggtggc
      541 ggcggagtcc cgggaggtag cggaggttcg ggcgggggca gcggcgctgg cgccggaact
      601 gctggacttc cgggcggaaa tctttgggaa tctggaactg gcgggggaag cggcggttcg
      661 ggtagcgcgg gaggcggtgg ggctgcgaac ggcttcgtgc catccggtac tgcgggaaag
      721 ggcggcaatt cctttcccgc tctctccact gcggctgcgg ccctgggtgc tgccgctccg
      781 gcaaatccca gcaaatggct gactgcgtcg gctctgctgc actccggctc tgctggaggc
      841 gccgctgagt ttaccgtgca agaagaagaa atccgctcga tgctcagtct cttcgcccag
      901 ctcggccgcg cgcgcaagga cgccggcagc gggggaatgg acgcgacgac ttttcaatcg
      961 cgcctttccg cgatgcctgt gcgcaaccag gttaccctgc agcaagctct tgccggcctc
     1021 gcggcgcaag ctcctacgga gaagcccgac cagccaatgc tcttgaagct tgcagagcac
     1081 gtcgccattc gcttcgctct ggacagctac gaaaaaggcg aacttcgcgt taatgcggtc
     1141 aagcagttgc tcgatcgcat gaacaccgaa atcgaagccc tgcgcaaaat cctcacctcg
     1201 caggaagagc aaatggcctc tgccgggctg aacgtccagt cctacactga actgctcgat
     1261 caggaattct gggaacaggt acccgacgaa aacaagaaag aagtcttggc atcggacgaa
     1321 gcctggtgtg tccctccgcg caacgtgcgc ggttttctgg aagaaatgtt gcgccgcggc
     1381 gaactcaaaa ccgtcaacga aattttaatg aagtacgccg cctgcatcgg cctggaaccg
     1441 gaagaagcgc gccgcaccac cgccatcggc ctgggcgaac tcgccgaact ctacggcagc
     1501 ggcgacggca gcgccttgat cgaaagtatt cgccgcgtcg gcaaccagct ggcggccgaa
     1561 cgcgatccgg aattgcaaac tttgatcagc gccgcgtttg tccgccttag ccaggaagcc
     1621 ggttcgaagc gctgctatcc cgccatgcag caggcgcttg cttcgctcga tagcgtcgag
     1681 gcccagcgcc ccggtgcgtc gcaaaatctc cgcccgcgca tcggcgccga agaacgcatc
     1741 cccgaattcg tcgaggagac cttgcgtacc ggcgaaatcg ccgacggcct gctggacatt
     1801 ctgctgctca tgcccaaggc cacgatcaac tacatcacca atcgcttcgg ccattctggc
     1861 ttccgcgacg attgcgaact cctggccgat ctggtcaaag gtttgggcga agatgccgtc
     1921 caccgcctcg ctgaaacgct gcaaaccgcc tccgccaatg aagctgccga agtggtcggc
     1981 ctgcttggcc agctcgatcc cgactctgtc gcacgcgttc tcccggttcg cctctcgcaa
     2041 tggccgcgtt ccgtgcacga tcgcgtcatt cgccagcttt gcactgttcc gcaggaatcg
     2101 cgcggcattt tgctgctctc catctttgac gcgctcgata gcctcatcaa gccgctcgcg
     2161 ctcgacgaaa ttggcatgac tggcctgccc gacgccattc acgccctcct cgatcttgcc
     2221 tccagcgatg gcatcggcgg ctacatccga ctgaaggcca tcgaagcctt gggtcgactc
     2281 cgcgcaacgg cggccgctcc gtccttgcaa acgattctgg aggcccgcca ggtctggcgc
     2341 tggcaatatc ccgccgagtt gcgcgttgct gcagcgcaag ccctgcagcg catcgatccc
     2401 gtcaccacta tgcaacgcct cgaagcctgg ggctttgaga aaaaagatct cgccctcgaa
     2461 cccaccgatc ccgaagccaa cagcgccgta atccgtcagc gccgctatgc tcgtctcaaa
     2521 ctcaatcgca acttggcctt catcaccacc aacttgcggg aaaattttag gctcaatgtc
     2581 gttgagttga atctgggcgg cggcattggc tccggtgaac gccatctcgc tcccggcacg
     2641 ctagtccact tcaagttgag ccagggcgtc cgcaccatca aggctcaggc gctcgtccgc
     2701 ggcgctcgtc cgcaagctat ggcttttgaa ttcgtagata tggacctcga agatcgcgcc
     2761 cgcttgcgca aaatcctcct cgaactcggc ggcctgcccc aggccgctca cgtggccagc
     2821 cgcgcgcgcc gccgcggccg catggccgtt ctcaagtgac cttcctagga gtccgctgtg
     2881 ttccaatcgc ctggccggtt ggcgcagtct ccgcgaaacc gctacaattg atatttgcgt
     2941 actcctcgcc ggacttggtc gtgtgtccgg tcgcggattc attcgcttct caggaaacga
     3001 cacacacttc atggacgccg ctaaaattct cgaccgcgtg gttgcctctt tcatcggcac
     3061 caagcacgaa cgcgacatca agaaacttca gcccgtagtc gctgccatca gcgccaaaga
     3121 gccggagatg caactgctct ccgatgacgc gctcaaatcc gaattcgcca agctgaaaga
     3181 cgaagttcag gcgcggctca aggacgtcga tgcctcggaa ccgggctatc gggaaaaaat
     3241 gcaggcggcg ctcgagccgg cgatcgtccc gtctttcgct ctcgctcgcg aagcgggccg
     3301 ccgtttctta aacatgcgcc atttcgacgt ccagctcatc ggcggactcg ttctccacga
     3361 cggcaaaatc gccgaaatga aaaccggtga aggcaagacc ctcgtcgcca ccctccccgc
     3421 cgttctcaac tctctcgcca gtcgcggcgt ccacatcgtc accgtcaacg actatctcgc
     3481 tcgtcgcgac gccgagtgga tgagccctct ctaccgcggt ctgggcctca ccattggagt
     3541 tatcgtccat gacctcgacg acgcgcaacg ccgtgcggcc tacggagccg acatcaccta
     3601 cggcaccaac aacgaatacg gcttcgacta cctccgcgac aacatgaagt acgacatcac
     3661 ccactgcgtt cagcgcggcc accagtttgc catcgtcgac gaagtcgact ccatcctcat
     3721 cgacgaagcc cgtactccgc tcatcatttc cggacccgcc gaggaatcca ccgacaagta
     3781 ctaccgcgtc gacaaaatca ttcccaagct gatcaaggaa attgactaca ccatcgatga
     3841 aaagcaccgc accgccacgt tgacggaaga aggcgtcagc aagaccgaac gcctcatggg
     3901 gctcggcaac ttatacgacc ctgcgcacat ggaaatcatc catcacgttt atcaggcatt
     3961 gcgcgcg
//
LOCUS       DASTPM010000323         4153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_732035, whole genome shotgun sequence.
ACCESSION   DASTPM010000323 DASTPM010000000
VERSION     DASTPM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732035"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFR42_05675"
     CDS             complement(<1..425)
                     /locus_tag="VFR42_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368676.1"
                     /translation="MRWLRLLLLGLASIYPLYWTAQFLLYFCPESAVGYWLGQPVRVI
                     SISYLQATAVTHPHPVFPAYWEAMVSACFFSLLILALRGDRFVSGAFSIVVLGQAALL
                     PFLNAFPSPTESRVTVVLGGLAAFLLIVLGLYRILSCTGG"
     gene            complement(493..1599)
                     /gene="leuB"
                     /locus_tag="VFR42_05680"
     CDS             complement(493..1599)
                     /gene="leuB"
                     /locus_tag="VFR42_05680"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU0368677.1"
                     /translation="MKKTIVLLPGDGIGPEVTAAAGNVLKECAREFHHQFEFQEQPIG
                     GAAIDLTGTPLPNATIDACRKADAVFLGAVGGPKWDSLPVGKRPESGLLALRQGLGLY
                     VNVRPVKVLEPLRSMSPLKPERLGDLDVEIVRELAGGMYFGERGNVAEGGKERSWDTE
                     SYSTAEVERIAAFAYNRAENRSRRLCSVDKANVLASSQLWRRTVTAMSSSYPNVQLEH
                     MYVDNAAMQLTLRPSQFDVMLTTNMFGDILSDAAAALVGSIGLVPSASFGSGAPLFEP
                     IHGSAPTLAGKDSANPIGSILSVTMMLRDSFGLGLEADWVEQSVVRVLAAGYRTPDLA
                     APGSKPTGTNGFMEILHVEMQRTLEHAERYGWGV"
     gene            complement(1617..2810)
                     /locus_tag="VFR42_05685"
     CDS             complement(1617..2810)
                     /locus_tag="VFR42_05685"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU0368678.1"
                     /translation="MDVVRIFDTTLRDGEQSPGFSMNTEEKIRMARQLAKLGVDIIEA
                     GFPISSRGDLEAVQAVAREVRDVPIAALARAKKVDLDAAIEALRPAASSRLHIFLATS
                     DLHLKVKLNMTREQALEAITSMVRFGRQHVAEVEFSAEDAGRTEIDYLCQVCRAAVDA
                     GASILNLPDTVGYSVPEESGGMFLKVREHLGDTPSVTLSAHCHDDLGLAVANSLAAVR
                     AGVRQIECTVNGIGERAGNASLEEIAVALAVRKENFGVTTNIKLDQLFPASRMLTEIT
                     GAQVAPNKAVVGANAFAHEAGIHQDGIIKNPLTYEIISPETVGVPARSLVMGKHSGRN
                     ALRVSLRDLGFEPSDAELAEIYRRVTTLADQSKTVRPRDILGIAHEVIRRGGSSVAAE
                     ASSAA"
     gene            2914..3789
                     /locus_tag="VFR42_05690"
     CDS             2914..3789
                     /locus_tag="VFR42_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0368679.1"
                     /translation="MDLSELQVFLTVAREGSFSRAAERLYRTQPAVSLAIRKLEDGLG
                     QPLFVRGARPVRMTDAGNLLRDYAERLINLRDEVKKGLSELEGLTRGELSLGVNESSI
                     HALLPALAQFREAHSGVQIRVHRMFSRDIPHEVLNYRLDLGAVSFVPRDAQLQATEIL
                     KDELTLVVPPKHPLAKKREVDVSDLGGESFVAHSVESPFRRRVIELFGRNKTTLNMPI
                     EMPTLESIKRFVQMGMGVAIVPRMCVAWEIDHGWMKEVKVRQLNLPRHVYMISRRGAR
                     LPHAAAELLRLLKPD"
     gene            complement(3931..>4153)
                     /locus_tag="VFR42_05695"
     CDS             complement(3931..>4153)
                     /locus_tag="VFR42_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368680.1"
                     /translation="PDLDGIQLLFDISGWPYTFPTLGSYPVSGSRLFCVTMIAFSTLL
                     GLRMYFLRWPATFPLALCPSRGPVRCLFL"
BASE COUNT          891 a         1193 c         1277 g          792 t
ORIGIN      
        1 ccgcctgtac agctcaaaat ccggtacagg ccaaggacaa tcagcagaaa agccgccagt
       61 ccgcccaaaa ccaccgttac gcgagactcg gttggagacg gaaaggcgtt taagaacggc
      121 aaaagcgccg cctggccgag cacgacaatg gaaaaggcac cggagacgaa gcgatcgccg
      181 cgcaaagcca agatcaacag ggaaaaaaag caggccgaaa ccatggcttc ccagtatgcc
      241 gggaaaaccg gatgcgggtg agttacggcg gtggcttgca agtaggagat gctgatcacg
      301 cgaacgggct ggccgagcca atagccgact gcgctttccg ggcagaaata caaaaggaac
      361 tgagccgtcc aataaagcgg ataaatggac gcgaggccca gcagcaatag acggagccag
      421 cgcattctgc ccctcagcag gggagtgatt ttgtcgcgac acagcggcat ttgcaagacc
      481 cagaactatc gatcagacgc cccagccgta gcgctcggcg tgctccagcg tgcgctgcat
      541 ttcgacgtgg agaatttcca taaacccatt ggttccagtg ggtttggagc caggcgcggc
      601 aaggtcgggc gtgcgataac cagctgcaag tacccgcacg acggactgct cgacccagtc
      661 ggcttcaagg cccagtccaa acgagtcgcg aagcatcatg gtgacgctca gaatggagcc
      721 aatgggattg gcggagtctt ttccggctaa tgtaggcgcg gaaccgtgga tcggctcaaa
      781 caagggcgcg ccggagccaa agctggccga cggaaccagt ccaatcgagc cgaccagggc
      841 cgctgctgcg tcgctcagga tgtcgccaaa catgttcgtg gtgagcatca cgtcaaattg
      901 cgagggtctc aacgttagct gcatggctgc gttgtcgaca tacatgtgct ccagctggac
      961 gttagggtag gagctgctca tcgccgtgac cgtgcgacgc cagagttgcg aactagccag
     1021 gacgttggcc ttgtccaccg aacaaagccg ccgcgagcga ttttctgcgc ggttgtaggc
     1081 aaaggccgcg atgcgctcga cttccgctgt ggaataggac tcggtatccc atgaacgctc
     1141 tttaccgcct tcggcgacgt tgccacgctc gccgaagtac ataccgccgg cgagttctct
     1201 gacgatttcg acatccagat cgccgaggcg ctcgggcttg agcggcgaca tgctgcgcag
     1261 aggctccaag acctttacag gccggacgtt cacatagagg cccaagccct ggcgcaaagc
     1321 cagcagaccg ctctccgggc gtttgcccac gggcagcgaa tcccatttgg ggccacccac
     1381 cgcgccgaga aaaaccgcat ctgctttgcg gcacgcgtcg atggtggcgt tcggcaaggg
     1441 cgttccggtc aagtcgatgg cggcgccgcc gatgggctgc tcctgaaact caaattgatg
     1501 atggaattcg cgcgcgcact ctttgagtac gttgccagcc gcagccgtca cttccgggcc
     1561 gatcccgtcg cccggcagaa gtacgatggt ctttttcatc gctgtaccgt gccgcactat
     1621 gccgcggaac tagcttctgc cgccaccgac gaaccgccgc ggcggatcac ttcgtgcgca
     1681 atgccaagga tatcgcgtgg gcggacggtt ttggactggt cggccagcgt agtcacgcgg
     1741 cggtagattt ccgccagttc ggcgtcgctc ggttcaaagc ccaggtcgcg caagctcaca
     1801 cgcagcgcat tgcggccgga gtgcttgccc atgaccagcg agcgcgccgg cacgccgaca
     1861 gtttccggag aaataatttc gtaagtaaga ggattcttga tgatgccgtc ctggtgaatg
     1921 ccggcttcgt gagcgaatgc gttcgcgccg acgaccgctt tgttgggagc aacctgggcg
     1981 ccagtaattt cggtgagcat gcggctggcg ggaaaaagct gatcgagctt aatgttggtg
     2041 gtgacgccga aattttcttt gcgcactgcc agcgctactg caatttcttc caaagaagcg
     2101 ttccctgcgc gctcgccgat gccgttgacg gtgcactcga tttgtcgcac accggcgcga
     2161 acagcggcca gcgagttcgc gacggccaag ccgagatcat cgtggcagtg ggcgctgagc
     2221 gtgacgctag gcgtgtcacc gagatgttcg cgcactttca gaaacatccc gccagattct
     2281 tccgggaccg agtagccgac ggtgtccggc aaattcaaga tgctggcgcc agcatccacg
     2341 gcggctctgc atacctggca gagatagtcg atttcggtgc ggcctgcatc ctcagcggaa
     2401 aattccactt cggcgacatg ctggcggcca aagcgaacca tggaggtgat cgcctcaagc
     2461 gcctgctcgc gcgtcatgtt gagtttgacc ttcaggtgca ggtcggaggt cgccaagaaa
     2521 atgtgtaaac gcgaggaggc ggcgggccgc aaggcttcga tggccgcgtc gaggtccact
     2581 tttttcgcgc gggccagcgc tgcaatgggc acatcacgaa cttcccgcgc cacggcttga
     2641 actgcttcca gatcgccgcg cgaagaaatc ggaaatccgg cttcgataat gtccacgccc
     2701 agcttggcca gctgcctggc catgcgtatc ttctcctcgg tattcatcga gaagccgggc
     2761 gactgctcgc cgtcgcggag tgtggtgtcg aagatgcgca ctacgtccat ggagtgctcc
     2821 tgttagtagt aggtcagaac agccccattt ggtcaaattt atctttattc tacttttgat
     2881 aatttcacct tataatgcct ggcgaaggat ttcatggatc tgagcgaatt acaggtgttt
     2941 ttgacggttg cccgggaagg cagcttctcg cgagccgcgg aacggttata ccggacgcaa
     3001 ccggcggtca gcctggccat ccgcaagctg gaagacgggc tggggcagcc acttttcgtg
     3061 cggggagcgc ggccggtgcg gatgaccgat gctggcaacc tgttgcgaga ctatgccgag
     3121 aggttgatca atcttcgcga cgaagtgaaa aagggcttgt ccgaactgga aggcctgacg
     3181 cgcggcgagc tttctctggg cgtgaacgaa agctccatcc acgcgctact tcccgctttg
     3241 gcgcagttcc gcgaggccca ttcgggcgtg cagattcgag tgcatcgcat gttctcccgc
     3301 gatatcccgc atgaagtgct gaattaccgg ctggacctgg gcgcggtttc atttgtgccg
     3361 cgcgacgcgc agttgcaggc cacggaaatc ctgaaagacg aattgacatt ggtggtgcct
     3421 cccaagcatc cgctggccaa aaagcgagag gtggatgtgt ccgacctggg cggggagagt
     3481 ttcgttgccc acagtgtcga gtcgccattt cgcaggcggg taatcgaact cttcgggcgc
     3541 aataagacca cgctgaatat gcctatcgaa atgcccacgc tcgagagcat caagcgcttc
     3601 gtgcaaatgg gcatgggcgt agccatcgtg ccgcgcatgt gtgtggcgtg ggaaatcgat
     3661 cacggctgga tgaaagaagt gaaggtgcgc cagctcaact tgccacggca cgtatacatg
     3721 atttcgcgcc gcggcgcccg cttgccacat gcggccgcgg aacttctacg gctgcttaag
     3781 ccggactagc ctggggggct tcgcgcgcgg tagcatgtcg cgcatcccta tcgtcaaccg
     3841 caatttgttc gcacttgtcc ttgcgctgtc aactgcaaac cgtgcttgag aagttggact
     3901 ttgccgctac aagtattgtc cccgcgagag ctacaagaat aagcagcgaa ctggaccgcg
     3961 gctcgggcac agcgctaatg gaaaggtcgc cggccaccgc aaaaagtaca tccggagtcc
     4021 cagcaaagtg ctgaacgcaa tcatcgtaac gcagaacagg cgcgagcccg agaccgggta
     4081 agagccgagt gtcgggaatg tataaggcca accggaaatg tcgaacaaaa gctggatgcc
     4141 gtccaggtcc gga
//
LOCUS       DASTPM010000324         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1164899, whole genome shotgun sequence.
ACCESSION   DASTPM010000324 DASTPM010000000
VERSION     DASTPM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164899"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..863
                     /locus_tag="VFR42_05700"
     CDS             90..863
                     /locus_tag="VFR42_05700"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU0368681.1"
                     /translation="MSHEIQPKPRVGIPWRTREEERQRSDEKLNYYFEAVRRAGGEPE
                     HISLASNDAELRKQAAELDGFVLPGSPADVDPARYRETRHAKTVTLDPDRDRTDSTLL
                     EHALAAHKPVLAICYGCQILNVHQGGTLLQDIPAEKPGTDAHGKTDLAASATKGDLEH
                     GATLAADSHLQKLAGAREVKINSSHHQAIDKPGYKLRVTARGTDGIVEGVELESHQDW
                     VIGVQWHPERLPDDAFAQKLFADFVGAARKAKLETTVKR"
     gene            975..1739
                     /locus_tag="VFR42_05705"
     CDS             975..1739
                     /locus_tag="VFR42_05705"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0368682.1"
                     /translation="MISLAGKAALITGGSRGIGAAAVKLFAQAGADVVFNYNKAKDAA
                     AQIEAEARKHGTRVEAFRADVSKHADNKKLVDQTIARLGRIDILVANAGVWNVEDLPI
                     EKMSEKQWDDMVRINLKSVYSVVHFAVPQMIKQKSGRIIPISSTAGQRGESFHTHYGA
                     TKGGIISFTKGLAMELAGHGILVNSVAPGWVATDMSNPVLDTKAGQKMAAAAIPLGRA
                     ATAEEVAGPILFLASDLANFMTGEIINVNGGSVLCG"
     gene            1736..2602
                     /locus_tag="VFR42_05710"
     CDS             1736..2602
                     /locus_tag="VFR42_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368683.1"
                     /translation="MTFAQDAGRIVARLACVALLFLAAITPRARGQNPDTLMPEASAS
                     KAKQILAQMIDAFGGAAYLHARQSECVGRMSQFGHNADLTAYLPFRSYWEFPDKNRTD
                     IGKKGNVIDLYVGYEGWTLDHDGVSEEPAAQVDNFREQLPNNPHYIFRYRLKEEGMNY
                     RWAGLDLIDLKPIDWVELSDSKQRMYRIAVARDTHLMVRFVVLTPNEANSQPDEDITS
                     YANYHALDGVQTPLQVSRTHNGRRVFQAFYDTCRYNPNLPANFFTKAGLEERFREVGS
                     RSDKKKAAKERE"
BASE COUNT          622 a          711 c          897 g          521 t
ORIGIN      
        1 cctctaacta gctgataata aataggttaa actagtactc gacggcggtt gccgctctct
       61 ctcgccaatg ctacgatttt gcgtctccca tgtcccatga aattcagcca aaaccgaggg
      121 tgggtatccc ctggcgcacc cgcgaagaag agcgccagcg cagcgacgag aaactgaact
      181 attacttcga ggcggtgcgg cgtgccggcg gtgaaccgga gcacatttcg ctggcttcga
      241 acgacgcgga gttgcggaag caggcggcgg agctggatgg gtttgttttg ccggggagtc
      301 ccgcggatgt ggacccggcg cgctaccgcg aaacgcgcca cgcgaaaacg gtaactctgg
      361 atccggatcg cgaccgtacc gacagcacgc tgctggaaca cgcgctggcg gcgcacaaac
      421 cggtgctggc gatttgctat ggctgccaga ttctgaatgt gcaccagggc ggcactctgt
      481 tgcaggacat tccggcggaa aagcccggaa cagatgcgca cgggaaaacg gatctcgcgg
      541 caagtgcgac caaaggtgat ttagagcatg gcgcgaccct tgcggcggac agccatttgc
      601 aaaagctggc tggcgcaagg gaagtgaaga tcaacagcag ccatcaccag gcgattgaca
      661 agccgggtta caaactgcgc gtgacggcgc gcgggaccga cggaatcgtc gaaggcgtgg
      721 aattggagtc gcaccaagat tgggtgattg gcgttcagtg gcatccggaa cgcctgccgg
      781 acgatgcgtt tgcgcagaag ctgtttgcgg atttcgttgg cgcggcacgc aaggcgaagc
      841 tggaaacaac tgtgaagcgc taaggacttt agcgtaagga tttttgcggt gacggaaaca
      901 cttggaatcg tgctgacacc gcgcactgcc tgaaggctgt gccacgacga gttcttaaat
      961 tcattggggg aatcatgatt tctttggcag gcaaggctgc tttgatcact ggaggttcgc
     1021 gcggaatcgg cgcggcggcg gtgaaactct tcgcgcaggc cggcgcggat gttgtcttca
     1081 actacaacaa ggcgaaggac gcggccgcgc agatcgaagc agaagcgcgc aagcatggaa
     1141 cgcgtgtgga agcattcagg gcggacgtga gcaaacacgc cgacaacaaa aaactggtgg
     1201 atcagaccat tgcgcggctg gggcgcatcg atattctggt cgcgaatgct ggagtctgga
     1261 acgtggaaga tttgccgatc gaaaaaatga gcgaaaagca gtgggacgat atggtgcgga
     1321 ttaatctaaa aagcgtctat tcggtggttc attttgccgt gccgcaaatg atcaagcaga
     1381 agtccggaag aattattccg atcagctcga cagcaggcca acgcggcgag tcgtttcaca
     1441 cgcactacgg cgcgaccaaa ggtggcatca tcagcttcac caagggcctg gccatggagc
     1501 tggcggggca cgggatcctg gtgaacagcg tggcgccggg gtgggtggcc acggacatgt
     1561 cgaacccggt actggacacc aaggcggggc aaaagatggc ggccgcggcg attccgctgg
     1621 gccgtgcggc gacggcggaa gaggttgccg ggccaattct ttttcttgcg tcggacctgg
     1681 ccaacttcat gaccggcgaa atcatcaatg taaacggagg ctcggtgctt tgcggatgac
     1741 gtttgcccag gacgcgggca gaattgtcgc gcgcttggcg tgcgtggcgc tgctatttct
     1801 cgcggcgatt acgccgcgcg cccgcggaca gaatccggat acgttgatgc cggaagcgag
     1861 cgccagcaag gccaaacaga ttctggccca gatgattgat gcgttcggag gggcggcgta
     1921 tttgcatgcg cgacaatccg agtgtgtcgg ccgcatgtcg cagttcgggc acaatgcaga
     1981 tttgaccgcc taccttcctt tcaggagcta ctgggaattc cccgataaga accggacgga
     2041 catcggaaaa aaagggaacg tgatcgatct gtacgtcgga tacgaaggtt ggacgctcga
     2101 tcacgacggc gtgagcgaag agcctgcggc gcaggtggac aattttcgcg agcagctgcc
     2161 aaacaatccg cattacattt tccgctaccg gctgaaggaa gaaggaatga attaccgctg
     2221 ggccggcctc gacttgatcg acctgaaacc gatcgactgg gtggagctga gcgacagcaa
     2281 gcagcgcatg taccggatcg cggtggcgag agacacgcac ctgatggtgc ggtttgtggt
     2341 gttgacgccg aacgaagcca acagccagcc ggacgaagat attacgtcgt acgcgaacta
     2401 tcatgcgctc gatggagtgc agacgccgct gcaggtttcc cgcacgcaca acggccggag
     2461 agtgttccag gcgttttacg atacgtgtcg gtacaacccg aatttgccgg cgaacttttt
     2521 caccaaggcc gggttggaag agcgtttccg cgaagtgggc agccggtcgg acaaaaagaa
     2581 ggcggccaaa gaacgggagt gaagtgatcc aattttccgt gggatcagtg accagttttc
     2641 cgtggtcggt gttctgtcag agaagagagt tcggttgcgc cgttcgagtg ggctcccgta
     2701 ggtttccgtt ctttcctgta cacgcattgg cggcagccca cgccccgttt t
//
LOCUS       DASTPM010000325         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_155332, whole genome shotgun sequence.
ACCESSION   DASTPM010000325 DASTPM010000000
VERSION     DASTPM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155332"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VFR42_05715"
     CDS             <1..1477
                     /locus_tag="VFR42_05715"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU0368684.1"
                     /translation="HSAKLGSAVALWSPARENAAGSSSYAIPSVFVDAVAASPTFNKL
                     VITALVVWAFGFSLILVGLVVGLARLAWVSANAKPIFDEDWMRTVLELSQFHKLARSV
                     RLLQCSSALAMPLTWGIFRPVIVLPSGATLWTEDRRRIVLCHELAHIARNDWLLQICA
                     EVARCFYWFHPLVWLAAARLRQESERASDDAVLRSGIAPSHYASQLLDLARTLENSGR
                     AWSTALAIAARPSNLERRFAAMLNPSINRRTLATRTKFLVPFLALTFLLPLAALRLTA
                     QNLSGKVSGSIHDPSDTGVANATIIVSNHAGDTIDMITMTTSDRDGNFSFKTLPAGEY
                     ELKVMKRGFEPFRLPQVTLESGRDFFQSITLQIGAIMEEVEVIPNGTAKPLPSGATGK
                     PSRIRVGGDLEAPNLITKVQPVYPESARSAGVSGTVILHAIISQDGKPLSLRVMNSQV
                     DPDLARSAVEAVSQWRYTPTLLNGEPIEVDTSIMVNFSLAP"
BASE COUNT          382 a          681 c          498 g          452 t
ORIGIN      
        1 gcattccgcc aaactcggca gcgcagtcgc tctctggagc cctgcgcgcg agaacgcggc
       61 aggctcgagc tcttacgcaa tcccttccgt ctttgttgac gccgtagccg cctctccaac
      121 cttcaacaaa cttgttatca ccgctcttgt cgtttgggca ttcggttttt cgctcatcct
      181 cgtggggctt gtagtaggtc tagcgcgcct cgcttgggtc tcagcgaacg cgaaacccat
      241 cttcgacgag gattggatgc gcaccgtttt ggaactttcc cagttccaca aactcgcgcg
      301 ctccgtccgc cttctgcaat gcagcagtgc tctagccatg ccgctcacgt ggggaatctt
      361 ccgccccgtc atcgtgctgc cgtccggcgc tacgctttgg actgaggacc gccgccgcat
      421 cgtgctttgt catgaacttg cgcatatcgc ccgcaatgac tggctcctgc agatttgcgc
      481 cgaagttgct cgctgcttct actggttcca tcctctcgtt tggctggctg ctgcgcggct
      541 gcgccaggaa agcgaacgcg cctccgatga cgccgtcctg cgctccggaa tcgcgccttc
      601 ccactacgcc agtcaacttc tcgacctcgc aaggacgctt gaaaactccg gccgcgcctg
      661 gtccaccgct ctagcaatcg ccgcccgccc atccaatctc gaaaggagat ttgccgctat
      721 gctgaatccg tctatcaatc gccgtactct ggccacgaga acgaaattcc tcgttccgtt
      781 tctcgctctt acgttcctgc ttcccctcgc cgctcttcgc ctcaccgcac agaatctctc
      841 cggcaaagtt agcggatcta tccacgatcc cagcgacacc ggcgttgcta acgccaccat
      901 catcgtgtcc aatcacgcgg gcgacacaat tgatatgatc actatgacca cctctgatcg
      961 agacggcaac ttcagcttca aaacactccc cgccggcgaa tacgaactaa aagtcatgaa
     1021 gcgcggcttc gaaccctttc gacttccgca agtcactctc gaatccggcc gggatttctt
     1081 tcaaagcatt accttgcaaa tcggcgcaat catggaggaa gtcgaggtca tccccaacgg
     1141 gaccgcgaag cctctacctt ccggtgccac tggcaaaccg tctcgcattc gcgtcggggg
     1201 tgaccttgaa gctcccaatc tcattacgaa agttcagccc gtctatcccg aatcagccag
     1261 gtcggccggc gtcagcggca ccgtcatcct ccatgcgatc attagccagg atggcaagcc
     1321 tctttccctc cgtgtcatga acagccaagt cgatcctgat ctcgcgcgct ccgcggtcga
     1381 agccgtcagc cagtggcgtt atacccccac actcctcaat ggtgaaccga tcgaagtcga
     1441 caccagcatc atggtgaatt tctcactcgc cccctagact cacttcatcg cctacgcgaa
     1501 cggtacccgc acgcggtggg aaattttccg attttgcact ctcggccaaa cgatgttgaa
     1561 ttccggccaa gcgatgcttc aagtcacaca ctgtcgggtt ccctccgata aagcagccgc
     1621 tcgcttcgga agcagccgtc ctcgcttttg ggtggcccag aaaaagcgga gggagtggcc
     1681 cgtgaggacg aatcattctt gacggacctt ttgggtcgcc agggtttgcg gttatcttac
     1741 aggctctgag ccgcgcccca tccggagccc tcacgcccta ggctggccgc ttgccaacga
     1801 ccggagcctg cgcacttcct actcttacgg tgcgcattca agattcgcgg cgtcgtttgt
     1861 atcgccgctt cccttgtact tggccgttaa ggcttctacg ggcaatggct cccccgtccc
     1921 tgaatcgtgg cacgagaatt gacggcgcca ggtccaggtt agcgcgcgct tcgcaaccca
     1981 tagatggcac aatctaaacc cggcggcgat tga
//
LOCUS       DASTPM010000326         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_155376, whole genome shotgun sequence.
ACCESSION   DASTPM010000326 DASTPM010000000
VERSION     DASTPM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155376"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VFR42_05720"
     CDS             complement(<1..1469)
                     /locus_tag="VFR42_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0368685.1"
                     /translation="MKHVVDPEGRRLPIKLDTTSNGEFAPVALSPANRAGNRLAQEAA
                     SQNAKRLGLSRRDFLISACGAASALLAFNAANAAASKRGGFFELEPEAALESQLAQAR
                     VGGKGEFIFDVQGHFVDPTGAWVKTAPADAFKWSPKAACGLAAKPGARSYLNCLGPEE
                     FVKDVFLDSDTDMMVLSFVPSRRDSEPLTIQAADAVRRIVDRMEGTHRLLLHGRVNPN
                     QPGDLEAMDELKERWKVCAWKTYTQWGVDGNGFFLSDDVGTRFIERARALGVKVICVH
                     KGLPFGKQSYEHSQCSDIGVVAKRFPDVSFLIYHSGFVTGVPERAYTGRGAGDGIDTL
                     IRSLVENGVKPDSNVYAELGSTWRFLMRDPDGAAHALGKLIKYCGENNVLWGTDSIWY
                     GSPQDQIQAFRTFQISPELRAQHGYPEVTPVLRAKIFGLNAARVYAISGDEVKKHTRA
                     DAVARERIAYREHPEPHYLTYGPKTRREFLNLLGWNGGSR"
     gene            1862..2161
                     /locus_tag="VFR42_05725"
     CDS             1862..2161
                     /locus_tag="VFR42_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU0368686.1"
                     /translation="MGNKLYVGGLPYSVTEGRLQEIFSLHGTVESANVISDKFTGQSR
                     GFGFVEMSSSKEAQKAIESLNGTQLDGRTLTVNEAKPMARRDGGGGGRDGGRNRW"
     gene            2406..2774
                     /locus_tag="VFR42_05730"
     CDS             2406..2774
                     /locus_tag="VFR42_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368687.1"
                     /translation="MTEREVVKQTAELLRGIIRSIDKKLEYSLVDQTQEGRFSLRLSS
                     RGREGIVSILTDDLRMAGQNAVRKNAVRQKIKSTRDHLLSNYVVDVMGRKVARMLKKA
                     GAAKDEYKPSFFRPSQGRRR"
     gene            complement(2877..3074)
                     /locus_tag="VFR42_05735"
     CDS             complement(2877..3074)
                     /locus_tag="VFR42_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368688.1"
                     /translation="MYRILCDRSPARELGSGLTWLKKDGRIVEFSTMEEAGAKAKELN
                     EAVTVAKVKYTAKAYDVGGDE"
BASE COUNT          674 a          907 c          959 g          699 t
ORIGIN      
        1 cgcgatccgc cgttccagcc gagcagattc aggaattcgc gccgggtctt cggcccgtaa
       61 gtgagatagt gtggctccgg gtgctcgcgg taggcgatgc gctcgcgggc gacggcgtcg
      121 gctcgggtgt gtttttttac ctcatcgccg ctgatcgcgt agacccgggc cgcgttcagg
      181 ccgaagattt tggcgcgcag caccggggtg acctcggggt agccgtgctg cgcgcgcagt
      241 tcgggagaga tctgaaaagt gcgaaacgcc tggatctggt cctgtggcga gccgtaccag
      301 atggagtcgg tgccccagag cacattgttc tcgccgcagt acttgatgag cttgcccagc
      361 gcatgcgccg cgccgtcggg gtcgcgcatg aggaagcgcc aggtgctgcc gagctcggcg
      421 tacacgttgc tgtcgggttt gacgccgttc tccaccagtg agcggatgag ggtgtcgata
      481 ccgtcgccgg cgccccgccc ggtgtacgcg cgctcgggga cgccggtgac gaaacccgag
      541 tgatagatga gaaagctcac atccgggaag cgcttcgcca cgacgccgat atcgctgcac
      601 tggctgtgct cgtaggactg tttgccgaag ggcagtcctt tgtgcacgca gatcactttc
      661 acgccgagcg cgcgcgccct ctcgatgaag cgcgtgccga cgtcgtccga caggaaaaag
      721 ccattgccat ccacgcccca ctgggtgtag gtcttccacg cgcacacctt ccagcgctct
      781 ttcagctcat ccatggcctc gagatcgccg ggctgattcg ggttgacgcg gccgtggagc
      841 agcaggcgat gtgtgccttc catgcggtcg acgatgcggc gcaccgcatc ggccgcctgg
      901 atcgtgagcg gctcggaatc acggcgcgaa ggcacaaacg acagcaccat catgtcggta
      961 tcgctgtcga gaaacacgtc cttcacgaat tcctcgggtc ccaggcagtt gaggtaactg
     1021 cgcgcgccgg gcttggcggc tagcccgcaa gccgctttgg gcgaccactt gaaagcatcg
     1081 gcgggcgccg tcttcaccca cgcaccggtt ggatcgacga agtgcccttg cacgtcgaag
     1141 ataaactcac ccttgccgcc gactcgcgcc tgtgcgagct gggactccag ggcggcttca
     1201 ggttcgagct cgaagaagcc gccgcgcttg ctggctgcgg cgttggccgc attgaaagcg
     1261 agcaacgcgc tcgccgcgcc gcaagccgaa atcaggaaat cacggcggct aaggcccagt
     1321 cgcttcgcgt tttggcttgc cgcctcctgc gccaggcggt tgccggcgcg gttggccggc
     1381 gagagcgcaa cgggcgcgaa ttccccgttc gaagtcgtat ccagcttgat cggcagccgc
     1441 cggccttcgg gatcaaccac gtgcttcatg ggaatgagta tagaccaaga ggtgacgggt
     1501 ggcgaatgag gaaagcagat ttttttcgaa gtcccgtgag tcgtttagcc tgttcagtca
     1561 cctctgtcca cttctctcca ctcagcaggg tgccacggaa tcacgcaaga acaaaaatcg
     1621 cactagccgt tgacagcatc tctattaaca catacagtat aattcctact tcagtataag
     1681 gccttctgaa agaggaagac acaagcgttg aaggcctcca aatcgtctct ccttgcgaaa
     1741 cgatctctaa aggacaatcc gatcagagcg tggttgcagt aattgagttc ccatgttctg
     1801 ccccattagc agtctaattc tcagggtcaa cgacacaaac attctctcat aggaggagaa
     1861 catgggtaat aaattatatg ttggcggtct gccttattca gtaaccgaag ggcgactgca
     1921 ggaaatcttt tcattgcacg ggaccgtcga atcggccaac gtgatctcgg ataagtttac
     1981 gggacagtcg cggggcttcg gcttcgtgga gatgagctcg agtaaggagg cccaaaaggc
     2041 catcgagtcg ctgaacggca cgcagctcga cgggcgcacc ctgaccgtga atgaagccaa
     2101 gcctatggcg cggcgtgacg gcggtggcgg aggccgcgac ggcggacgta atcgctggta
     2161 gtcgttccgt tttatagcta atcacctggg ggcaataggt cgggttgccc ctcttttctc
     2221 gcacaaaacg agtcggcttg tgccgattcg ccgggagtag ggtttattac ttccgcgatt
     2281 ctaagcgttg actctctgtt aggcgcgccg taacagagtt gttcttgctc caagttagtc
     2341 aggctccgag gacattcggt aaccttggag gctttttatt gagccgcaaa cagggggagg
     2401 ttcccatgac tgaacgcgaa gtggttaaac agaccgcaga gttattaaga ggtattatcc
     2461 gcagcataga caaaaagctt gagtacagct tagtggacca gactcaagaa gggaggttct
     2521 ctcttcggct gtcatcacgc gggcgggaag gaatcgtttc catactgact gacgatttgc
     2581 gaatggccgg acagaatgcc gtgcgcaaga atgcggtccg gcaaaagatc aagagcaccc
     2641 gcgaccattt gctatccaac tatgtcgtag acgtcatggg gagaaaagtt gcgaggatgc
     2701 tgaaaaaggc cggtgcggcg aaggatgaat acaagccgtc cttctttaga ccctcgcagg
     2761 gaagacgaag gtaacaggac cgtggatttt gccgccgacg atagcgatgc tccgtgagcc
     2821 attgtttgcg gaggtgccgc gactcgtggc agttcggata aatttgcctt aatagattac
     2881 tcgtctcctc ccacgtcata tgctttcgcc gtgtacttta ctttggccac ggtgacagcc
     2941 tcattcaact cctttgcttt cgctccggct tcttccattg tggagaactc tactatccgg
     3001 ccgtccttct taagccacgt caggcccgag ccgagctcgc gagccggcga tctgtcgcac
     3061 aaaatcctgt acatttgaac cctcctcgtt aaattgtcct gcttaatctg atcgcgtttg
     3121 ggaaacgcgc ggaaagatca ctaaacaatg ctaaggcttt tcggttgagt aagcaaggag
     3181 ataccggcta atttcttcct aactcagtgt aggttcctta ttccttgaac cttgaactc
//
LOCUS       DASTPM010000327         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_905301, whole genome shotgun sequence.
ACCESSION   DASTPM010000327 DASTPM010000000
VERSION     DASTPM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905301"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..303)
                     /locus_tag="VFR42_05740"
     CDS             complement(151..303)
                     /locus_tag="VFR42_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368689.1"
                     /translation="MRDFLKSSREATDLRLRELTRIEQRRLADEVAMAIARLKEHAAA
                     HGCKRR"
     gene            complement(913..1776)
                     /locus_tag="VFR42_05745"
     CDS             complement(913..1776)
                     /locus_tag="VFR42_05745"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEU0368690.1"
                     /translation="MSIDAIKTVAVLGAGTMGNGIAHVFARAGYSVILRDVGQRYLDL
                     ALETIGKNLDREVKKGKLAESEKSSVLARFKPVTDMAAIASADFVVEAVPEKLEIKRA
                     VLGEADKILRAEVVIASNTSSISMTALAALTSRPDRFVGMHFMNPVPVMVLVEVIRAL
                     QTSDAAFELTMELAKKLGKTPVAVRDAPGFVSNRVLMPLINEAAYCVMEGVAAPEAVD
                     TVMKLGMNHPMGPLELADFIGLDVCVDILHVLHDGLGDPKYRACPLLKKYVAAGWLGR
                     KTSHGFYTYER"
     gene            complement(1845..2444)
                     /locus_tag="VFR42_05750"
     CDS             complement(1845..2444)
                     /locus_tag="VFR42_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368691.1"
                     /translation="MKSAAVGFRVHSGWTALVAVALQKGKPIVLARQRPHLVETFSYA
                     FRQPYHTAEKMALEQAREFLAKQQAEARCYAVAAIRAAERDVAQQGYEVTRAALLLAS
                     GRQLPELAKILAAHSLIHSADGELFREALLDGCAQCRLPVTCFKERELLGIASDEFRR
                     KPAALAKVAGDLGKPLGSPWTQDEKFATLAAWTALVKTT"
     gene            2599..3801
                     /locus_tag="VFR42_05755"
     CDS             2599..3801
                     /locus_tag="VFR42_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEU0368692.1"
                     /translation="MPVGTPFHERTLALCESLNYREWSGYYSVSVYETHHEHEYNAIR
                     NSAALIDISPLFKYRITGRDATRLVNRVITRDINKVAVDQVIYCCWCDSEGKVVDDGT
                     ITRLGENNYRWTAADPSLRWFQQNALGLDVAMEDISEQTAALALQGPTSGKLLHAAAD
                     ANIGNLKYFRVTHGKIAGVQVDISRTGYTGDLGYEIWVPADQAVKVFDALMAEGKPFD
                     IHPAGMIALDVARIEAGLILIEIDYTSSKKALIPSQKFTPGEIGLGKLVDLKKENFVG
                     RDALVAEAKKGGAPRALVGLEVDWNEVEALFDKQGMAPQVPSMASRLAVPVFRAGRQV
                     GKATSTTWSPTLKKMIALATVSREHSAVGTNLHMEMTVEAVRSTVAVNVVPLPFFNPP
                     RKTATPVM"
     gene            3868..>4974
                     /locus_tag="VFR42_05760"
     CDS             3868..>4974
                     /locus_tag="VFR42_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0368693.1"
                     /translation="MPENVNEGKLSRRNFLEASSVALAAAGVNSVANLPAQDQNQPGY
                     ARRKSDPGPTNPEIDAQNQSSFDPPETDAGGVPPFKYPFSFSHKRLHSGGWSREVTLR
                     ELPVSKSMAGVDMRLTAGGIRELHWHTAAEWAFMLYGSARITAIDNEGKSFVADVKEN
                     DLWYFPTGIPHSIQGLNPDGAEFILVFDDGNFSEYATVLLSDWMAHTPKDVLAKNFGL
                     PQSSFAKLPAKELFIFQSAVPGSLDADQKAAAGKRGLSSQDFAFRTMQSPPTNRTKGG
                     EVRVIDSRTFKAAANIAAAIVTIKPGGIRELHWHQNADEWQYFISGKGRMTVFATGGR
                     ARTMNFQAGDVGYIEKTLPHYVENTGDTDLKFLEM"
BASE COUNT         1102 a         1642 c         1229 g         1001 t
ORIGIN      
        1 atatcgcggc ttccaggagt tttgcttttt cgtcggacat tacgcacctc aatagaggaa
       61 attgggaaat tgctttgccg gatctctcgt cctcggcgga taaacgtagt ttacagtatc
      121 ggcacgttac ctgccactat attctgtcgc ttaccgccgc ttacaaccgt gtgcggccgc
      181 atgctccttc aagcgggcga tggccatggc cacttcatcg gccaaccgcc gctgctcgat
      241 ccgcgtcaat tcccgtaacc gcaggtcagt cgcttcgcgg ctgctcttca aaaagtctcg
      301 catgcggtcc cgggctgcga tgtattcctc ttcgagccta gcatgctctg aacaagagat
      361 tgcacgggcc ggagaagagc acatggcgca cctgttgaaa acgtcgaagg agaagttagc
      421 attatggcga acaaaaggca aacattattt tcgcctatta ttcgctgatg atctggaaga
      481 tccataggga cacccgccag aatgcaactg taaagccccc gccggttttc ggtgtgcttt
      541 gtcaaaagtc aacccgggca agctgaccat tcgaacagag atacacctgc gccgtccccg
      601 gcgtgagagc gcgcagagaa atgttcgtaa ataatcgctg ggcgagacta atccgtagtc
      661 gccttctcgg aaaaataact aaactttggg aatttgacgg ttttacgtcc gtttccacgt
      721 aatcgttcat gtctcgatcg acccacactt cgtgcccctg ggcatccgtt gcgggccgct
      781 tctgctcgcc gataacccag agttcaggcg ctcgagtgaa cgtatgacga ccgaacagga
      841 ttgcgaagac taaggcgaac gttgctaata gaatcaggca acggcgcgag gattcttcac
      901 gggacagtgc cctcacctct cataggtgta gaaaccgtgg ctagttttgc ggcctaacca
      961 tccagccgca acatacttct tcagcagggg gcacgcgcga tatttaggat cgcccaggcc
     1021 atcatgcagg acatgaagaa tgtccacgca aacgtccagc ccgatgaagt cggccagctc
     1081 cagcgggccc atgggatgat tcatacccag cttcatcacc gtatccacgg cttcgggcgc
     1141 ggccacacct tccatcacgc aatacgccgc ttcgttgatc agcggcatca gcactcggtt
     1201 cgacacaaag cccggcgcgt cgcgcaccgc cacgggagtc ttccccaact tcttggccag
     1261 ttccatcgtc aactcgaacg ctgcgtccga agtctgtagc gcgcggatta cttccaccag
     1321 aaccatcacc ggcaccggat tcatgaagtg catccccaca aatcgatccg gccgtgacgt
     1381 caaggcagcc agcgccgtca ttgaaatcga agacgtattg ctggcgatga ccacctcagc
     1441 acgcaaaatc ttgtccgcct cacccagcac cgcccttttg atctccaatt tttccggcac
     1501 cgcttccacc acaaaatccg cgctggcaat cgcagccatg tcggtcacgg gtttgaaccg
     1561 cgcaagcacc gacgattttt ccgattcggc cagcttccct tttttcactt cgcgatcaag
     1621 attcttccca atggtctcga gcgctagatc caggtagcgc tgccccacat cgcgcaaaat
     1681 cacgctataa cccgcgcgcg caaacacgtg cgcaatgccg tttcccatgg tcccggcgcc
     1741 caacaccgcc accgtcttga tcgcatcaat cgacatacgt tctccagaaa aagcccataa
     1801 agaaaagcat ttggagcggc caaggtcaag ctggagacaa taggctaagt ggttttgacc
     1861 agcgcggtcc acgcggcgag cgttgcgaac ttttcgtcct gggtccacgg cgagcccagc
     1921 ggtttgccga ggtctcccgc tactttcgca agcgcagcgg gcttacggcg aaactcgtca
     1981 gatgcaatac caagcagttc acgctcctta aagcaggtga caggaagtct gcattgagcg
     2041 cagccgtcca gcagcgcttc gcgaaataac tcgccgtcgg cgctgtggat gagcgaatgc
     2101 gccgcaagta tcttggccag ttcgggcagc tggcgtccgg aagcaagcaa cagggccgct
     2161 cgcgtaactt cataaccctg ctgcgcaacg tccctttccg cagcgcgaat cgccgcgact
     2221 gcataacatc gcgcctccgc ctgctgcttt gccaaaaact ccctcgcttg ttccagcgcc
     2281 attttttcgg ccgtgtgata cggctgtcgg aacgcataag aaaacgtctc caccagatgc
     2341 ggccgttgcc gcgccagcac gatcggcttt cctttttgca aagctaccgc aaccagcgcc
     2401 gtccagccgg aatgtacgcg aaaaccaacc gccgcactct tcatggaacc tcctagtgga
     2461 gccagtggag gtcgaggaat ttcgggcttt cagcatgcct cgcgcactgc gaacggtcaa
     2521 gcaatagaaa tgtcggccga gctttacgcc gccacgcctg tttccctata atcgctggcg
     2581 acttccggag gcctcgcttt gcccgtcggc acgccctttc acgaacgaac cctcgccctc
     2641 tgcgagagcc tgaactaccg cgaatggtcc ggctactact ccgttagcgt ctacgaaact
     2701 caccacgagc acgagtacaa cgccatccgc aactctgctg cactgatcga tatctctccc
     2761 ctcttcaagt accgcatcac cggccgcgac gccacccgcc tcgtcaatcg cgttatcacg
     2821 cgcgacatca acaaagtcgc cgtcgaccag gttatctact gctgctggtg cgattccgaa
     2881 ggtaaagtag tcgacgacgg caccatcacc cgcctcggcg aaaacaatta ccgctggacc
     2941 gccgccgatc ctagtctccg ctggtttcag caaaacgctc tcggcctcga cgtagcaatg
     3001 gaagacattt ccgaacaaac cgcggcgctg gcactgcagg gcccgacctc cggcaaactc
     3061 cttcacgccg ccgccgacgc caacatcggt aacctcaagt attttcgtgt cacccacgga
     3121 aagatcgccg gcgtccaagt agacatttcc cgcaccggct ataccggcga cctcggctac
     3181 gaaatctggg ttcccgccga ccaagcagta aaagtctttg acgcgctgat ggccgagggc
     3241 aagcccttcg acatccatcc tgccggcatg atcgctctcg acgtcgcccg catcgaagcc
     3301 ggcctcatcc tcatcgagat cgactacact tccagcaaga aagctctcat tccttcacaa
     3361 aagtttactc cgggcgaaat cggcctcggc aaacttgtcg atctgaaaaa ggaaaatttt
     3421 gtcggacgcg acgccctggt cgccgaagcc aaaaagggtg gagcccctcg cgctcttgtc
     3481 ggcctcgaag tcgactggaa cgaagtcgaa gcccttttcg acaagcaagg catggccccg
     3541 caagttccca gcatggcttc tcgcctagcc gttcccgttt tccgcgctgg tcgccaggtc
     3601 ggcaaagcca cttccaccac ctggtcgccc acgctaaaga aaatgatcgc cctcgccacc
     3661 gtcagccgtg agcactcggc cgtcggcacc aatcttcaca tggaaatgac cgttgaagcc
     3721 gtccgcagca ccgttgccgt caacgtcgtt ccgcttccct ttttcaatcc gccacgcaag
     3781 accgcaacgc ccgttatgta agcgcgcgcc gccgaaccgc attcgttgca ccgttgctct
     3841 caatttcccc gggaaaggat gtttgccgtg cctgaaaacg tcaacgaagg aaaactctct
     3901 cgccgcaatt ttctggaagc cagctctgtg gcgctcgccg cggccggcgt caattccgta
     3961 gccaatcttc ccgcgcaaga ccagaatcag cccggctacg cgcggcgtaa gagcgatcct
     4021 ggccccacca atccggaaat cgacgcgcaa aaccaatctt cgtttgatcc tcccgaaacc
     4081 gacgccggcg gcgttcctcc gttcaaatat cccttttctt tttcgcacaa acgtctgcac
     4141 agcggcggct ggtcccgcga ggtcaccctg cgcgaactcc ccgtctccaa atccatggct
     4201 ggcgtagaca tgcgcctcac tgccggcggc attcgcgaac tgcactggca caccgcagcc
     4261 gaatgggctt tcatgctcta cggcagcgcc cgcatcaccg ccatagacaa cgagggaaaa
     4321 agttttgtcg ccgatgtaaa agaaaacgac ctctggtact tccccaccgg cattccccac
     4381 tccattcagg gactcaatcc cgatggcgcc gagttcatcc tggtcttcga cgacggcaac
     4441 ttctccgaat acgccacggt cttgctctcg gactggatgg cgcacacgcc gaaagacgtt
     4501 cttgccaaga acttcgggct gcctcaatca tctttcgcca agctgccggc caaggaactc
     4561 ttcatcttcc aatccgccgt tcccggctcg ctcgatgccg atcagaaagc ggccgccggc
     4621 aagcgcggcc tttcctcaca agacttcgcc ttccgcacga tgcagtcacc accgaccaat
     4681 cgcactaaag gtggcgaagt ccgcgtcatc gattcgcgta ccttcaaagc cgccgccaac
     4741 atcgcggccg ccatcgtcac cataaaaccc ggcggcatac gcgaattgca ctggcaccag
     4801 aacgccgacg agtggcaata cttcatctct ggcaaagggc gcatgaccgt tttcgctacc
     4861 ggtggccgcg cgcgcaccat gaatttccag gccggcgatg tcggctacat tgaaaaaacg
     4921 ctgccgcact acgtcgaaaa caccggcgac accgatctga aatttctgga aatg
//
LOCUS       DASTPM010000328         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_818643, whole genome shotgun sequence.
ACCESSION   DASTPM010000328 DASTPM010000000
VERSION     DASTPM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818643"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFR42_05765"
     CDS             complement(<1..1007)
                     /locus_tag="VFR42_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368694.1"
                     /translation="MPLTTTDWAVIIGYLLINLLIGIYYRQRSSGNTAEFFISGRDVS
                     WWLAGTSMVATTFAADTPLLVTGLVATQGIAGNWLWWNQCLTGMLTVFFFARYWRRAE
                     IMTDVELTELRYSGKPAAFLRGLKAVYLGLLMNCFIVGWVTRAMVDIIAVLLGPAIAA
                     GKVWQMTLGDHVLLHYTLGEPRHTALLICVFVLIPFTGIYTFIGGLWGVLVTDLFQFI
                     LKMGMVIVLAWIAVAKIGGIEALALRLQVVSEATARSGATVANPLAFFPDFGHGFTSE
                     AVWTIPLISFVLFLGVQWWCSWYPGAEPGGGGYVAQNMLACKDERNSRAAALFFNVAH
                     YA"
     gene            complement(1144..1695)
                     /locus_tag="VFR42_05770"
     CDS             complement(1144..1695)
                     /locus_tag="VFR42_05770"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU0368695.1"
                     /translation="MTVARQVWGFIERRDHRVMRRMNRWRAPRWIRYWMLAATRAGDG
                     WLWYSLGFALLAFGGTQRFAAVGAAGSAALVGVGVFKILKRLSHRPRPCQIEPHCWSR
                     VLPPDRFSFPSGHTMTAFSIALVLSYFYRGVELPLYFMAASIAISRVVLGMHFLSDVL
                     AGAILGVGLGCASLTAFAALGFI"
     gene            complement(1753..2628)
                     /locus_tag="VFR42_05775"
     CDS             complement(1753..2628)
                     /locus_tag="VFR42_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0368696.1"
                     /translation="MRCDLHVHSTASGMCDTPLLNRVCRESYNQPDEVYARCKHLGMS
                     IVTLTDHDSIDAAQRLRRHTDFFVSEEVTCRMPSGTLVHIGVYNLSDRQHLEIQNRRN
                     SFVSLLMYLTEQKLFFSVNHMFSGLTGKRDAEDFHWFASYAPAFEVRNGQMWGPANQQ
                     AQKLAKRLSKIGIAGSDSHTITGVGLTYTEVKGASTVSEFFAGLRARRGRVKGIEGTY
                     SKLTADIYRIGAAMFQEKPATLAMLPLSVLVPAFTAAHWLNEVWFCKKWEKHFADGEK
                     APRMLWDLRAGAERV"
     gene            2793..>3924
                     /locus_tag="VFR42_05780"
     CDS             2793..>3924
                     /locus_tag="VFR42_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0368697.1"
                     /translation="MSSKRKIHVLFHDAGGGHRNAAVALKAVCEQQQRPWDINPVQFQ
                     ELTDHLDVLRRLTGIRIQEQYNILLRNGWTWGSEYLLRVLQLTIRVLHRPMVKLLANY
                     WRAHPADLLISVIPHFNREIAESWTSVYPGRRFVTIITDMADFPPRFWIEPIAEQIVV
                     AGSDRAAQQARGLGKTEKNLFQASGMILRPEFYVEQEESCTAIRQRLGLDLQLPTAIV
                     LFGGYGSNVVFDIAEQLDAARLPLQLILICGKNEKLAARLQAKQWNIPVHVVGFTREV
                     NRLMHAADFLIGKPGPGSIAEAVTSGLPVLLECNSSTLPQERYNTEWITEKQVGAVVK
                     HFKREIVGAVQGFLEPGRLQEMKRNVGVLDNRAVFEIPEFLSS"
BASE COUNT          898 a         1198 c         1059 g          769 t
ORIGIN      
        1 gcgtagtgcg cgacgttgaa gaagagcgcg gccgcgcggc tgtttcgctc gtccttgcag
       61 gcgagcatgt tctgcgcgac gtacccgccg ccgcctggtt ccgcccccgg ataccacgaa
      121 caccaccact gcacgcccaa aaacagcaca aacgaaatta gcggaatggt ccacaccgct
      181 tcactggtga agccatgacc gaagtccggg aaaaaagcca aaggatttgc aaccgtggcc
      241 ccgctccgag cggtcgcctc gctcacaact tgcaaacgaa gcgccagcgc ctcgattccg
      301 ccgatcttag ccaccgcaat ccacgccagc acaatgacca tgcccatttt caaaataaac
      361 tggaagaggt cagtgaccag cacgccccac aatccgccga tgaaggtata aatgccggtg
      421 aagggaatca gcacgaagac gcagatcaac agcgcggtgt ggcgcggctc gcccagcgtg
      481 taatgcagca aaacgtgatc gcccagcgtc atttgccaga cttttccagc ggcgatcgct
      541 ggccccaaca gcaccgcgat gatgtccacc atcgcgcgcg tcacccagcc gacgatgaag
      601 cagttcatca gcagcccgag gtagactgcc ttcagtccgc gcagaaaagc tgccggcttt
      661 cccgaatagc gcagctcggt gagctcgacg tcggtcataa tttccgcgcg acgccaatag
      721 cgcgcgaaga aaaacaccgt cagcattccg gtcaggcact ggttccacca cagccaattg
      781 cccgcaatcc cctgcgttgc gacaaggcca gttaccagca gcggcgtgtc cgcggcaaac
      841 gtcgtcgcca ccatactggt tcccgccagc caccatgaca cgtcgcgccc cgaaataaaa
      901 aattccgccg tgttcccgct cgaacgctgc cggtaataaa tcccgatcag caggttgatc
      961 agcaagtagc caatgatcac cgcccaatcg gttgtggtta gcggcatatt cctctcgtca
     1021 acacaggcga cctgggcgca agggcatgtt tctccgctct ggatgccaga ggatagtgct
     1081 atagagcatt aaaagcaagc gattttcagc gcgattcgcg gcgtcggcga aaacgcaggt
     1141 tgtctagatg aaacccaggg cggcgaaggc cgtgagcgat gcgcagccga ggccgacgcc
     1201 gaggatggct ccggcaagca cgtcgctcag gaagtgcata cctaaaacga cgcgggaaat
     1261 cgcgatgctg gccgccatga agtacagtgg caattccaca ccgcggtaga aatagctcag
     1321 caccagtgcg atggaaaacg cggtcatggt gtgaccggaa gggaaggaaa atctgtccgg
     1381 aggaagcact cgcgaccagc agtgcggctc aatctggcag ggccgcggcc ggtggctcaa
     1441 ccgcttcaaa atcttgaaca cgcctacgcc gaccaacgcc gcactgccag ccgcgccgac
     1501 tgccgcgaat ctctgtgtcc cgccaaatgc cagcagcgca aaccccagcg aataccacaa
     1561 ccagccatca cctgctcgcg tggccgcaag catccagtag cggatccagc gtggcgcacg
     1621 ccaacggttc atccggcgca tcacgcggtg atcgcggcgt tcgatgaatc cccaaacctg
     1681 acgtgcgacg gtcatggcct tctcccgtct tgtagcccgg ctagcaagac ttccactaca
     1741 tcagccacca cttcaaacgc gttcggcgcc cgcgcgcaaa tcccacagca tgcgcggagc
     1801 tttttctcca tccgcaaaat gcttttccca cttcttgcaa aaccaaactt cattcagcca
     1861 gtgcgccgcg gtgaaggccg gcaccagcac cgacagcgga agcattgcca gtgtcgccgg
     1921 tttttcctgg aacatggctg cgccgatgcg atagatatcg gcggtcaatt tcgaatacgt
     1981 tccctcgatg cctttcacgc ggccgcgccg cgcacgcagc ccggcaaaaa attcgcttac
     2041 cgtgctcgcg ccttttactt ccgtgtaggt cagtccgact ccggtgatgg tgtgcgagtc
     2101 gctccctgca atgccaatct tgctcagacg ttttgccagt ttctgtgctt gctggttggc
     2161 cggcccccac atctgtccat tgcgtacttc gaatgccggc gcatatgacg cgaaccagtg
     2221 aaaatcttcc gcgtcgcgct tccccgtcag accggaaaac atatggttca cgctgaagaa
     2281 cagcttctgc tcggtcaaat acatcagcag cgagacgaaa ctgttgcggc gattctggat
     2341 ttccaggtgt tgccgatccg acaggttgta aacgccaatg tgcactagcg tcccgctggg
     2401 catccggcac gtcacttcct cgcttacaaa aaagtctgta tggcgccgca gacgttgcgc
     2461 cgcgtcaatc gagtcatggt ccgtcaaagt gacgatggac atacccaggt gcttacaacg
     2521 ggcatagacc tcgtcaggtt ggttgtagga ctcgcggcac acccgattga gcaagggcgt
     2581 gtcgcacatg ccagaggcgg ttgagtggac gtgcaggtcg catctcatgc ccctcatttt
     2641 cccgcagaca tatgaacagt ccatgaagag gtagaaaaat ctgcatgaat gtgctcttgg
     2701 aactcattga acacaaatga cttctagagt tatccacatg gcatggaccg tgctcttatg
     2761 caaggcagag tttttcatgt gacactcctg tcatgtctag caaaaggaag attcacgttc
     2821 tgttccatga cgccggaggc ggccatcgca acgccgcggt ggccctgaag gccgtctgcg
     2881 aacagcagca gaggccttgg gacattaacc cggtccagtt tcaggagttg accgaccatc
     2941 tggacgtgct tcgcaggctc accggcatcc gtattcagga gcagtacaac attctcctgc
     3001 gtaatggctg gacttggggc agtgaatacc tcctacgcgt actgcaactc accattcgtg
     3061 tcttgcaccg cccgatggtc aagctgttgg cgaattattg gcgcgcgcat cccgcggatc
     3121 tgctgatctc ggtcatcccg cacttcaacc gcgaaattgc cgaaagctgg acctccgtct
     3181 atcccggccg gcggttcgtc accatcatca ccgacatggc ggactttcca ccgcgcttct
     3241 ggattgaacc aatcgccgaa caaattgtcg tggctggctc ggaccgcgcc gcccaacaag
     3301 ctcgcggctt gggaaagaca gaaaaaaatc tatttcaagc ttccggaatg attctgcgtc
     3361 cggaatttta tgtggagcag gaggagtcct gtacggcaat ccgccagcgg ctaggactcg
     3421 atcttcaatt gcctacggcg attgttctat ttggcggtta tggctccaat gtggttttcg
     3481 acattgcgga acaacttgac gcagcgcgat tgccgctaca attgattctg atctgcggca
     3541 agaatgaaaa actcgcggcc cgcctgcagg cgaagcaatg gaacatcccc gtacacgtcg
     3601 tcggttttac gcgggaagta aaccggctga tgcatgcggc cgattttctg atcggcaagc
     3661 ccggcccggg aagcattgcc gaagccgtga cttcgggcct gccggttttg ctggaatgca
     3721 attccagcac cctgccgcaa gagcgttaca acactgaatg gatcaccgaa aagcaggtcg
     3781 gagcagtggt caaacacttc aagcgagaaa tcgttggcgc tgtgcaaggg ttcctcgaac
     3841 cgggacggct gcaggaaatg aaacgcaacg tcggcgttct tgacaaccgt gccgtctttg
     3901 aaatcccaga atttctgtcg agct
//
LOCUS       DASTPM010000329         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_703607, whole genome shotgun sequence.
ACCESSION   DASTPM010000329 DASTPM010000000
VERSION     DASTPM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703607"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFR42_05785"
     CDS             complement(<1..419)
                     /locus_tag="VFR42_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU0368698.1"
                     /translation="MSETAKIVAQESEGRESSQKAERGADAGFLWDFWYPAARSSAIE
                     GRRLATAMLLEVPLVLGRTDEGKAFAMRDSCPHRGIPLSYGRFDGKSLECSYHGWKFD
                     GCSGQCVEIPSLTSQDNLKVERIFAGHYPCEEHDGYV"
     gene            917..2008
                     /gene="prfA"
                     /locus_tag="VFR42_05790"
     CDS             917..2008
                     /gene="prfA"
                     /locus_tag="VFR42_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU0368699.1"
                     /translation="MAVLTLNEKLDQLGHRYEEMTQQLSTPEAVSDSARFQKLAKQHS
                     ELAEIVNKHREFKQIEKDLAGAHQMVLEAEDPEIKQMAHDEERQLTERREIVERELKL
                     LLLPKDPNDDKNVIVEIRAGTGGDEAALFAAELFRMYSRYAETQNWKVEILESSPSSL
                     GGLKEVVVAIAGSKVYSKLKYESGVHRVQRVPATETQGRIHTSAATVAVLPEADDIDI
                     KIEAKDLRIDTFCSSGPGGQSVNTTYSAVRITHIPSGLVVSQQDEKSQIKNRAKAMRV
                     LRSRLYEMELEKQQAALGAERRGQIKTGDRSEKIRTYNFPQNRVTDHRIGLTLHQLTD
                     VMEGKLAPLVDGLVTHYEAERLKQELAET"
     gene            2098..>2944
                     /gene="prmC"
                     /locus_tag="VFR42_05795"
     CDS             2098..>2944
                     /gene="prmC"
                     /locus_tag="VFR42_05795"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU0368700.1"
                     /translation="MDIRTALKLGIAHLRAANVPSDTLAAELLLLHSTGRNRTSLYSH
                     PEVPLTEAESQTYFRVLEQRAAGIPTQHLTGKQEFWGLEFEVTPEVLIPRPETEHLIE
                     VALDRLALRDLHTRFTQNMTGEGLRLADLGTGSGCIACALAKEFPAAEIYALDISPAA
                     LAVAQRNATRLGFSDRIHFHESNIFSALRESPVTSHKPPLFDLIASNPPYLGRREAVT
                     LAREVRDHEPEVALYGGEEGYELYSDLITQSAAQLKPGGILVVELGHDSLPAVQPLFD
                     SPDWTN"
BASE COUNT          658 a         1040 c          667 g          579 t
ORIGIN      
        1 cacacgtatc cgtcatgctc ctcgcacgga taatgtcccg caaaaatccg ctcgaccttc
       61 aggttgtcct gcgacgtcag cgacggaatc tccacacact gaccgctgca cccatcaaac
      121 ttccacccgt gataactgca ctccaggctt ttcccgtcga accggccata cgaaagcggt
      181 attccccggt gcggacaaga gtcccgcatc gcaaacgcct tcccctcatc ggtccgtccc
      241 agcaccagcg gcacttccag cagcatcgcc gttgccagcc gccgcccttc gatcgcagaa
      301 ctccgcgccg ccggatacca gaaatcccac aagaatcccg cgtccgcacc gcgctcggcc
      361 ttttgcgaag actcgcgccc ttcgctttct tgcgccacga tttttgccgt ttcgctcatt
      421 ttccttgact tatgttgcct ctgatgctaa tagagcttat gtgtactaga tttcggcccc
      481 gcccataggg ttgcgaacac ttccgccccg ctcctgtaag tccttttctt gccacacgtg
      541 caaggaatgc gtccgtaagt cctttgcctg ccgcacttac agcgtgccgg cccgcaattc
      601 ctttgcttgc cagactaagg aaaaatagtc aggggtaccc ccgactcgct ctcttcccga
      661 aatgaaactc gctgccctcc ccgcagcctt ttgcggcttc atcttacagc aatttctaga
      721 actcgatttg ccgctcccgt ccgccggctg ctatcataag aacgatcatg aagttctcct
      781 ctttctatta gccgtcccgc actcctcccc tcaggggatc ttgtcgcccc cattcgtctc
      841 gctcatttca actcaatcgg tctcttctct cacggaaaac tgagaacaga ccacggaaaa
      901 ctttttagag gtcaccatgg cggtccttac cttaaacgaa aaactcgatc agctcggcca
      961 tcgctacgaa gagatgaccc agcagctctc cacgccggaa gccgtttccg actccgcgcg
     1021 ctttcagaag ctcgccaaac agcactccga gctcgccgaa atcgtcaaca agcaccgcga
     1081 gttcaagcaa atcgaaaaag atctggctgg cgctcatcag atggtcctcg aagccgaaga
     1141 cccggaaata aagcagatgg ctcacgacga ggaaaggcag ctcaccgagc gccgagaaat
     1201 tgtcgaacgc gaactgaagc ttctgctgtt gcccaaagat cctaacgacg acaagaacgt
     1261 catcgtggaa attcgcgccg gcaccggtgg cgacgaagcc gctctctttg ccgccgaact
     1321 cttccgcatg tattcgcgct acgcggaaac gcaaaactgg aaagtggaaa ttctggaaag
     1381 ttctccgtcc tctttgggtg gcttgaaaga agtcgtcgtc gccatcgccg gcagcaaggt
     1441 ctattccaag ctgaagtacg aaagcggcgt ccatcgcgtc cagcgagtgc ccgctaccga
     1501 aacccagggc cgcattcaca cttccgctgc tactgtagcc gtccttccgg aagccgacga
     1561 catcgacatc aagatcgagg ccaaagattt gcgcatcgac accttctgct cctccggccc
     1621 cggcggtcag tctgtgaaca ccacctattc cgccgtgcgc attacccaca ttcccagcgg
     1681 cctggtcgtt tcccagcagg acgaaaaatc gcaaatcaaa aatcgagcca aggccatgcg
     1741 tgttctacgc tcgcgtctct atgaaatgga attggaaaag cagcaagccg ctctcggcgc
     1801 cgaacgccgc ggacaaatca agaccggcga ccgctccgaa aaaatccgta cctacaactt
     1861 cccgcaaaac cgcgtcaccg atcaccgcat cggcctaacg ttgcaccagc tcaccgacgt
     1921 aatggaaggc aaactcgccc cgctcgtcga cggcctggtc acccactacg aagccgaacg
     1981 cctaaaacag gaactcgccg aaacctgatc aattcttggt attcgtccat gccagccaag
     2041 ccccaaaatc gtcattccga acggagcgag gaatctctct tcaccggctc ggcagtcttg
     2101 gacattcgga ccgcgctcaa actcggcatc gcgcacctgc gcgccgctaa cgtcccttcc
     2161 gacacgctcg ccgccgaact gctgctcctc cactccaccg gccgcaaccg cacttcgctg
     2221 tactcccatc cggaagttcc actcacagaa gccgagtcgc aaacttattt ccgcgtgctc
     2281 gaacaacgcg ccgcgggaat tcccacgcaa catctcaccg gcaaacagga attttggggc
     2341 ctcgaatttg aagttactcc agaggtgcta attccccgcc cggaaaccga gcacctcatc
     2401 gaagtcgctc tcgaccgcct cgccctgcgc gacctgcaca ccagattcac ccaaaacatg
     2461 accggcgaag gcttacgctt ggcggacctc ggaaccggct ccggctgcat cgcctgcgcg
     2521 ctcgccaaag aattccccgc cgcagaaatc tacgcgctcg acatttcccc cgcggcttta
     2581 gccgtagcgc aacgcaacgc cacccgcctc ggcttctctg atcgcatcca cttccacgaa
     2641 tccaacatct tctctgcgct tcgcgagtca ccggtcacga gtcacaagcc acctctgttc
     2701 gatctcattg cttccaaccc gccctacctc ggccgccgcg aagcggttac gctggcccgc
     2761 gaagtccgcg accacgaacc tgaagtagcc ctctacggcg gcgaagaagg ctacgaactc
     2821 tactccgatc tgatcacaca atccgccgcg cagctgaagc cgggcggcat cctcgtagtc
     2881 gaactcggcc acgattccct tcccgccgtc caaccactct tcgattcacc cgactggacc
     2941 aacg
//
LOCUS       DASTPM010000330         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_818728, whole genome shotgun sequence.
ACCESSION   DASTPM010000330 DASTPM010000000
VERSION     DASTPM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818728"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /gene="uvrB"
                     /locus_tag="VFR42_05800"
     CDS             complement(<1..1626)
                     /gene="uvrB"
                     /locus_tag="VFR42_05800"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU0368701.1"
                     /translation="MDFKLKSAYQPRGDQPAAIDQLLRGVEAGDKHQVLLGVTGSGKT
                     FTMAKVIAASRRPALVLAHNKTLAAQLYHEFKSFFPENAVEYFVSYYDFYQPEAYVPA
                     ADVYIEKEATINDELDKLRMSATRSLFERRDVVIVASVSCIYGIGSPEAYYGMLVMLE
                     KGLQTSREALLRRFVDVQYERSEDLRRGTFRVRGDIIEIYPTYEDNAYRLEMWGDQID
                     KLRQIDPLTGEVRPGQQDLTRVPIYPRTHYVMPQDQRERAIVTIQEELEWWKKELEKQ
                     GKFVEAQRVTQRTLFDVEMMRTIGYCHGIENYSRHLSGRLPGEAPPTLLDYVPQDYLL
                     FIDESHQTVPQVRGMYHGDRARKQTLVDYGFRMPSALDNRPLNFEEFENRTNQIVYVS
                     ATPGPYELTKSGGIFVEQVIRPTGLVDPEVEIRPVKGQVDDLLEQIRIRAEKNERVLV
                     TTLTKRMAEDLSEYFTEIGVRCRYLHSEVETLDRIKILRDLRRGEFDVLIGINLLREG
                     LDLPEVSLVAILDADKEGYPRSATSLIQTIGRCA"
     gene            complement(1739..>2447)
                     /locus_tag="VFR42_05805"
     CDS             complement(1739..>2447)
                     /locus_tag="VFR42_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368702.1"
                     /translation="TVSGLRLKTYLSEKKTAGPSQSGAAENSSSPDKLVEVFISSNGS
                     ASRIKGLDQLSAPQQLAWSSWLSQFASPLTYPKPGVEKGRRWRASEAETADSPIAELS
                     WRRKYEYVRQEACPLAEQSSPPSKSDKSFASRQMCAVILVQAQLRQGSPANRSTPPDF
                     KLRGLSTRGTASGTNETVQYISTASGLIMRSIEDVQQSMDVTVALADGSNQVRYLINA
                     KSRLQIQLLPDVPQDIR"
BASE COUNT          509 a          659 c          746 g          533 t
ORIGIN      
        1 agcgcagcgc ccgatcgtct gaatcaaaga tgtggcgctg cgcgggtatc cctctttatc
       61 ggcgtcgaga atcgcaacca gcgaaacttc cggcaaatcg agcccttcac gaagcaggtt
      121 gatgccgatg agaacgtcga attcgccgcg gcgcaggtcg cgaagaattt tgatgcgatc
      181 gagtgtttcc acctcggaat gcagatagcg acagcggacg ccaatctcgg tgaagtattc
      241 gctgaggtcc tctgccatac gcttggtaag cgtggtgacc aggacgcgtt cgtttttctc
      301 ggcgcgaatg cgaatctgtt cgagcaggtc atccacctgg cccttgaccg gacgaatctc
      361 gacttccgga tcgaccaagc ccgtggggcg aatgacctgt tcgacaaaga taccgccgga
      421 cttggttaat tcgtatggtc cgggagtcgc cgaaacataa acgatctggt tggtgcgatt
      481 ttcgaattcc tcgaaattca gtgggcgatt gtcaagcgcc gaaggcatgc gaaagccgta
      541 gtccacgagc gtctgcttgc gcgcacggtc gccgtgatac atgccacgga cttgcggtac
      601 ggtctggtgc gactcgtcga tgaaaagcag atagtcctgc gggacgtagt cgagcaaagt
      661 gggcggagct tcgccgggta ggcggccact caggtggcgc gaataatttt caataccgtg
      721 gcagtaaccg atcgtgcgca tcatctcgac gtcgaagaga gtgcgctggg tcacgcgctg
      781 ggcttcgacg aatttgcctt gcttctcaag ctccttcttc caccattcca gttcttcctg
      841 aatggtgacg atggcgcgct cgcgctgatc ttgcggcatg acgtaatggg tgcggggata
      901 aatgggaaca cgcgtgagat cctgctggcc ggggcggact tcaccggtga gcggatcaat
      961 ctggcggagc ttgtcaatct ggtcgcccca catctccagg cggtaggcgt tatcttcgta
     1021 ggttgggtag atttcgatga tgtcgccgcg aacgcgaaac gtaccgcggc gcagatcttc
     1081 gctgcgctcg tattgcacgt cgacaaaccg gcgcaacaaa gcctcgcgac tggtctgcag
     1141 gcctttttcg agcatgacca gcatgccgta ataggcttcc ggcgagccaa tgccgtagat
     1201 gcagcttact gaagccacga tcacaacatc acgacgttcg aacagggaac gggtcgcgct
     1261 catgcgcagc ttgtcgagct cgtcgttaat cgtggcttcc ttttcaatgt aaacatcggc
     1321 ggccggtaca tacgcttccg gctgatagaa atcatagtag ctaacgaagt attccacagc
     1381 gttttctgga aaaaagctct tgaactcgtg ataaagctgc gcggccaagg ttttgttgtg
     1441 agcgagcacc agagccgggc gccgcgacgc cgcgatcact ttggccatgg tgaacgtctt
     1501 gccggaacca gttacaccca gcagcacctg atgcttgtcg cccgcctcca cgccgcgcag
     1561 gagttggtcg atggcggcgg gttggtcgcc gcgcggctgg tacgcactct tcagcttgaa
     1621 atccatcgga gctgctcctg tcggttagaa ggagagaaac gcaaacttcc attatagtcc
     1681 ggcaggcaag cgagtgcgca tggctgcgat aaaggccagc tgttcgcgga aagcggagtt
     1741 agcggatgtc ctgcgggaca tcgggcaaca actggatttg caggcggctc ttggcgttga
     1801 tgaggtagcg gacctgattc gagccatcgg cgagcgcaac ggtgacgtcc atggactgct
     1861 ggacgtcttc gatggagcgc atgatgaggc ctgaagcggt cgaaatgtat tgcacggttt
     1921 cgtttgtgcc gcttgcggta ccgcgcgtgg aaaggccgcg caatttaaaa tcgggcggag
     1981 tcgagcgatt ggccggagat ccctgacgaa gctgggcttg caccaagatg accgcacaca
     2041 tttggcgcga tgcaaatgat ttgtcgcttt tgctgggcgg ggaactctgt tcggctaggg
     2101 gacaggcttc ctgccggaca tattcatact tccgcctcca cgataattct gcgatcggag
     2161 agtcagccgt ttccgcttca gacgctcgcc aacggcgccc tttttcgacg ccgggcttcg
     2221 gatacgttaa cggagaggca aattggctta gccacgagct ccacgccagt tgctgaggag
     2281 ccgagagctg atcgagccct ttaatccggg atgcagagcc attagacgag atgaagactt
     2341 cgaccagttt gtcggggcta gacgaattct ccgcggcacc ggactgcgac gggccggccg
     2401 tttttttctc cgacaaatat gttttcaggc gaagcccgga gactgtc
//
LOCUS       DASTPM010000331         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_530595, whole genome shotgun sequence.
ACCESSION   DASTPM010000331 DASTPM010000000
VERSION     DASTPM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530595"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFR42_05810"
     CDS             <1..612
                     /locus_tag="VFR42_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368703.1"
                     /translation="RPLHQAIDSIRATNPAAGTSAIAHDWLFCALAERDGAAATQALS
                     ALNENEISLADKVSFNRHFVEGLIARMRNDERNAQLAFTAARAEQEKIVEAQPDFGPA
                     WCALGMIDAGLGRKEEALREGRRALELLPLEKDALVGQYLARYFAVIAAWVGEKDLAC
                     EQLAVVVQPPSNVSYGELKLMPWWDPLRGDPRFDKIVSSLAPK"
     gene            complement(644..949)
                     /locus_tag="VFR42_05815"
     CDS             complement(644..949)
                     /locus_tag="VFR42_05815"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HEU0368704.1"
                     /translation="MILTGSNIPTAEETGPDPVDTYRPADLEKLGIRSPQDFETFLPQ
                     EAGSTVNLNIGNGGDGTVQINLRGLLPKETLVLIDGKRVAFGSLGNAGSSQGDPGYN"
     gene            1612..1908
                     /locus_tag="VFR42_05820"
     CDS             1612..1908
                     /locus_tag="VFR42_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368705.1"
                     /translation="MSISAYGPMDEAGLWTFDGAGSFTASLVFNFGGGIILNTSWSQN
                     VTGTYTVNANGTGTMTLTGDHRRHFVIGDGGRQLKYVGTDPDGGIVVGGSMVKQ"
BASE COUNT          630 a          729 c          730 g          565 t
ORIGIN      
        1 cgcccattgc atcaggcaat cgattccatt cgggcgacaa atcccgccgc tggaacatct
       61 gctatcgctc atgattggct cttctgcgca ctagccgaac gcgatggcgc cgcggccaca
      121 caagccttga gcgcgctgaa cgaaaatgaa atttccctgg ccgataaggt ttcattcaat
      181 cgtcattttg tcgaaggtct catcgcgcgc atgagaaacg acgagcgcaa cgcgcaattg
      241 gcttttaccg ctgcacgcgc agagcaggag aaaatcgtcg aggcgcaacc cgatttcggc
      301 ccggcctggt gcgcgctggg catgatcgac gccggcctcg gccgaaaaga ggaagcgttg
      361 cgcgaaggcc gccgcgcact tgagcttctt ccgttggaaa aagatgcgct cgtcgggcag
      421 tacctcgcca gatacttcgc agtgattgca gcgtgggttg gagaaaaaga tctcgcctgc
      481 gaacagctcg ccgtcgtcgt ccagccgccc agcaacgtca gctatggcga actgaaactg
      541 atgccgtggt gggacccgtt gcgcggcgat ccacgcttcg acaaaatcgt aagttcgctc
      601 gctccgaagt gagtacaggt gatacgtgat ccgtgatcgg tgatcagttg tagccgggat
      661 cgccctggct agacccggcg ttgcctaacg aaccaaaggc gacacgtttt ccatcgatca
      721 gaaccaacgt ctctttaggg agtagaccac gcaagtttat ctgcacagta ccgtccccgc
      781 cattgccaat gttcaaattg accgtgctgc ctgcttcctg cggcaggaaa gtttcgaagt
      841 cctgtggact gcgaatgccc aatttctcga ggtccgccgg ccgataggtg tccaccgggt
      901 ccggcccggt ttcttctgcc gtcggaatat tcgaaccagt taaaatcaca cgctcgacct
      961 ccactgtgcc cggagcgcct aactcctgag caaacgcgtt cagtgcgaaa aagaaagaaa
     1021 ttgtaacgcg cgctgcgaga gcaccacgag aggagcgtga tgtggttagc gcgtgcattt
     1081 caggcccaac gggggtttca ctgaacacga ttacaagggt tcgcgctgaa atttgcgatc
     1141 tgaatcgtct accggtttaa ccaccgcgca cggtgtttca cttaagtcgg attgctcaag
     1201 ggcggctgac acttcgaaaa atcgccgcgg tccaccgcgg cgattaaaaa tgagacgagc
     1261 tttaactatt tcaacgtggc gaagccattt tccgcgtaac gaacggccga attcagccaa
     1321 gtacctctct gaacggccag aaatgatttt ctcacaacga gaagtcacgg acggccaaaa
     1381 caagcaagga attagtttat gaaaaaccaa aacattatgt tcacaagaag cgttcccaag
     1441 atgtcgaagg cccggctgtt caggtcggtt ctggcgctgg gggtagttgc attgatcacg
     1501 caaatctccc tgccgcgcgc ccaggcctat gacctgagta gcctcaacgg tagttatgcc
     1561 gactctttgt cgggcttttc tccactaagc cccgggggac ctaacaggcc cgtgtccata
     1621 agcgcctatg ggccaatgga cgaggcgggc ttgtggacgt tcgatggcgc aggcagcttt
     1681 acggcaagtc tggtctttaa cttcggtggc ggcatcatac tcaatacgag ttggagtcag
     1741 aatgtcaccg ggacctatac tgtgaatgcg aacggtaccg gcaccatgac tttgacgggg
     1801 gaccatcgcc gccacttcgt gatcggcgat ggcggaaggc agctcaaata tgtgggtaca
     1861 gaccctgacg gcggcatcgt agtcggcggt tccatggtca aacagtagcg ggcaatagct
     1921 ctgaagcagt tcacgatcag ccgtcacgct tcactgtgtg gcggctgatt gtttctccgt
     1981 ctgcgggaaa gcacgcgccc tagtgtgttg gcgattgcgc gctcgatggt atttctcggc
     2041 ttacagaaga caacaacgac aaaggctctc tttcgccgca accaaacgaa gtcttcgctg
     2101 tcttcgtttc cttctgttta gcaacctatc gcctatcggt gaaatccacg aaacctgtca
     2161 cgccgtctgc tcgcgaagac tttcggagta gcctctcgac ccgccgtagt cccgcgatgc
     2221 gggacgaagg ctggttggcg aaggaggatc agcggtctct cagcttcgac gcttgaacgg
     2281 atcaaccagt taacgattta acgcagcgaa gccattttca gcgtaacgaa ccgtcagatc
     2341 ccgccaagta ccacagtgaa cggccagaaa tgacaactca ataacgagga gtcaaagaag
     2401 gccaaaacca aaaaggaaat taaaaacacc tatgaagaca acactgataa agacgactct
     2461 tcgcggagct ttggcggctc tgcttgttct gacgttcaca cctttgctgg catccggggc
     2521 cggaaaaata gaaggggctt ggaatgtgaa cgtgacagtt aggaattgtg cgacagggca
     2581 gccagtggca actgtttaca ggatgcttac gtttgcggat gacggcgtaa tccaggaatc
     2641 cgccgccttc ttca
//
LOCUS       DASTPM010000332         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_876677, whole genome shotgun sequence.
ACCESSION   DASTPM010000332 DASTPM010000000
VERSION     DASTPM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876677"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..483
                     /locus_tag="VFR42_05825"
     CDS             49..483
                     /locus_tag="VFR42_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368706.1"
                     /translation="MPLLDQAARWTTNKMPKVINPKAHAIIDYAMAASFFGMAAFFWR
                     RNKRAAVSALVCGAAETITALSTDYPGGVVKEISYETHGAIDFGLSGMAASLPDMLRF
                     SDQPESRFFRVQGLALATVTGLTDFTGTGEGGQLQELDRRSA"
     gene            754..1053
                     /locus_tag="VFR42_05830"
     CDS             754..1053
                     /locus_tag="VFR42_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368707.1"
                     /translation="MSLKLAREMETETETRTFNDDFPALEEKIYKAIELLKSAREGKA
                     AAERDAGRLREQLETREEELDVLRGEMIALRKEREEVRGRVEKMLKQIDALVAEG"
     gene            1136..1516
                     /locus_tag="VFR42_05835"
     CDS             1136..1516
                     /locus_tag="VFR42_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEU0368708.1"
                     /translation="MKCFKAVWSEATKMGVQHNMTTTQFELHGFEVVQTLGMVRGIIV
                     RSRSIFGTIGASLQTLVGGNITLLTNLCERTRQEALDLMLQHAGQLGANAVVGVRYDA
                     TEIMQGVTEVLAYGTAVIVQQKRN"
     gene            1707..2018
                     /locus_tag="VFR42_05840"
     CDS             1707..2018
                     /locus_tag="VFR42_05840"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU0368709.1"
                     /translation="MTQPTAAPPVRVEIYDQVYNLRGVDAEYITKLAEFVDGKMRNVA
                     EQTSTIDSLRLAVLAALNIADEYHILKKKYDLLAGEFNRRAGQLAGALDEVLEDERRA
                     G"
     gene            2062..2478
                     /locus_tag="VFR42_05845"
     CDS             2062..2478
                     /locus_tag="VFR42_05845"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0368710.1"
                     /translation="MTEADLYRFISQCKLGVLSSVASSGTPQSALVGIAVSRDLEIIF
                     DTVKTSRKYGNLTARPACSFVFGWAAEQTVQYEGVAEELGGPQLQRSQQIYFQAWPDG
                     PSRMNWPGIVYFLVKPRWIRYSDFDQNPPLIREFAR"
     gene            2576..2769
                     /gene="ssrS"
                     /locus_tag="VFR42_05850"
     ncRNA           2576..2769
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFR42_05850"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          653 a          788 c          819 g          614 t
ORIGIN      
        1 tccctgccat gtttccatct ctctaactga atccccaagg agagacacat gccgcttctc
       61 gaccaggccg cccgctggac caccaacaaa atgccgaagg tcatcaaccc gaaggcccac
      121 gccatcattg actatgccat ggcggcttca ttctttggca tggcagcatt tttctggcgg
      181 cgaaacaagc gcgcagcggt aagcgccctg gtctgcggcg cggcggaaac cattaccgct
      241 ctcagcaccg actatcccgg cggcgtggtc aaggaaatca gctatgaaac ccatggcgcc
      301 attgactttg gcctgagcgg catggccgcc agccttcctg atatgcttcg cttcagcgac
      361 cagccggaat ctcgcttctt ccgggtgcag ggcctggccc tggccacagt taccggcctc
      421 acagacttca ccggcaccgg cgaaggcgga cagttgcaag agttggacag acggtcggca
      481 taagaaagca ctcagcagca ctcagccaaa cctgtttact gcaacgcatg cgaaggtcgc
      541 gaagccccgt cgttgtgagt gtggtcaacg cgcggatcat cgaacttaca gaattgttac
      601 agttcctttg cgctgcttgc gccctttgcg ataaaaccct cttggctgag tgctgaaggc
      661 tggttgctga gtgttatttc tttctttctc ctttccacaa cgcagtgcgc tatactccgc
      721 aaagaacgtc atcccccgag gtgctgctta ctcatgtcat tgaaacttgc tcgcgaaatg
      781 gaaactgaga cggaaacaag gactttcaac gacgattttc ccgcgctgga agaaaaaatc
      841 tataaagcca ttgagttgct gaagtcggcc cgtgagggca aagccgccgc ggagcgcgat
      901 gccggccggc tgcgcgagca actggaaacc cgcgaagaag agctggacgt gcttcgcggt
      961 gaaatgatcg ccctgcgcaa agagcgtgag gaagtgcgcg ggcgcgtgga aaagatgctc
     1021 aagcagattg acgcgctggt ggcagagggc tgaaaggttt cacttttggg cataggtgag
     1081 cgttgaggcg gcgaaatgcc gagccgattg cccaggagca gaaagctttg aaagggtgaa
     1141 atgctttaaa gcggtttgga gcgaagcgac aaaaatgggc gtccagcaca acatgaccac
     1201 cacgcagttt gagctgcatg gctttgaagt ggtgcaaaca ctgggcatgg tgcgcggaat
     1261 tattgtccgc tcacgttcga tttttggcac tattggcgcc agtttacaga cattggtcgg
     1321 cggaaatatc acgctgctga ccaatctttg cgaaaggacg cgccaggaag cactggattt
     1381 gatgctccag catgccgggc agctaggggc caatgcggtg gtcggcgtcc gttatgacgc
     1441 tacggagatc atgcagggcg taaccgaagt tttggcctat ggaacagcgg tcatagtcca
     1501 gcaaaaacgc aattgagcct ggtacagagg tagccgcgag ccctaccacg cggcgaggag
     1561 cttgcatcag gagccaatta aaccttgagt cgagagggct ctccagcggt tttggagcgc
     1621 agcgacaaaa ataaagactt gaccggtcgt caactcggac gataatcaat ccaacagaag
     1681 attttttttg aaggagaatg agcttggtga ctcaaccgac agcagcgccg cccgtccgag
     1741 tggaaattta cgatcaggtc tataacctcc gtggcgtgga tgcagaatac atcaccaagc
     1801 tggcggagtt tgttgacggc aagatgcgta acgtggccga gcagacttcg accatcgatt
     1861 ccctgcgtct ggccgttttg gcggcgctga atatcgccga tgaataccac atcctgaaaa
     1921 agaaatatga tctgctggcg ggcgagttca atcgccgtgc tggacagttg gccggcgcgc
     1981 tggatgaggt cctggaagac gagcgccgcg cgggatagca gaaggttgct tcgcgctgga
     2041 acgatttgag gacgacatgg catgacagaa gcagacctct atcgcttcat ctcgcaatgc
     2101 aaacttggtg tgttatcttc cgttgcgtcc tctgggacgc cgcaatcggc actggtcggc
     2161 atcgccgtct cccgagacct tgagatcatc ttcgacaccg tcaagacttc acgcaagtac
     2221 ggcaatctca ccgcccggcc tgcttgttcg ttcgtgttcg gctgggcggc cgagcagact
     2281 gtccaatatg aaggagttgc ggaagaactt ggcgggcccc agttgcagcg ttcgcaacag
     2341 atctacttcc aggcctggcc cgatggtcct tcccgtatga actggccggg aatcgtgtat
     2401 tttctagtga aaccacgatg gatccggtat agcgacttcg atcagaatcc tccgctgatc
     2461 cgtgaatttg cccgctgacc gacgctcgca ctccttgtgt caatcgccgg ttaaggagct
     2521 aaggcaatcc cggacttgcc atctactctg cttttcttta gcatcagtcc tgataaccgg
     2581 cctgcaacgc aggtgagaac ggtaaccgtt catgaaccaa cattcaatga acagggggcc
     2641 tgactcgtat aataacggct cggtgtgcga ggttccgcca gtacggaaat gcctaaagag
     2701 ccgcggcggg ttcccaccgt cttaggacgg gttcagaacg gcccggtcgc acggccaagt
     2761 gggtcggttg ttccattttt cctgtcgcta cgctccacac cgccataaac caacgaccgc
     2821 tttagttcct gatgctcctg atgcttgcgc ttcggcgcgc aagtcggcct cagc
//
LOCUS       DASTPM010000333         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_329021, whole genome shotgun sequence.
ACCESSION   DASTPM010000333 DASTPM010000000
VERSION     DASTPM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329021"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFR42_05855"
     CDS             <1..547
                     /locus_tag="VFR42_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0368711.1"
                     /translation="AEMSKKETVASALLRNLQFQEYEVRSAAEGMPEEKYSYRPAEGK
                     FNDEKPEFGPAEVRTFAEQVKHVACSNFAFAAELDGQKPPDACDKGGPSPARTKKELL
                     IYLRDSFAAIRKSFGVITAQNMYEPIEGPYAGPNTRLGLATVVIWHAADHYGQMTLYL
                     RLNGIVPPASRPNPPEVKDSY"
     gene            695..1042
                     /locus_tag="VFR42_05860"
     CDS             695..1042
                     /locus_tag="VFR42_05860"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU0368712.1"
                     /translation="MPRFLHQVSYTSEAMSRLIANPQDRFEAVRGPVEKLGGKIEHGY
                     FAFGEHDAILITDMPDNISAAAIALAFAAGGALRNCTTTPLLTTAEALDAMRKAAACG
                     YKPMQAAAGAASR"
     gene            1182..2003
                     /locus_tag="VFR42_05865"
     CDS             1182..2003
                     /locus_tag="VFR42_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEU0368713.1"
                     /translation="MPGEPRTAWLVNIRLHPIKGLDPVSVPEARIGPNGGLQLDRVWV
                     LFSVDGKWINGKNNPEIHRIRAEYDPLLGSVLLSVPVAGNSDNARGTLKSARLAFPSD
                     TERAAAWFSEYFSRTVIVRHIPEGAPDDGLATGPTIVSTASLQAVCDLCPGVELNEAR
                     ERFRTTLEIDGVPAFWEDQLFGEDENYPVRFKIGEVRFEGSNPCARCPVPPRNPRTGE
                     DLIGFQKKFTDMRRSQLPHTSPRERFDHFYRLATNTRVASTEQGKVLRVGDALVL"
BASE COUNT          453 a          654 c          624 g          442 t
ORIGIN      
        1 cgccgaaatg agcaagaaag aaaccgttgc ttcggccttg ctgcgcaacc tgcaattcca
       61 ggaatacgag gtacgctccg ccgcggaagg gatgccggaa gagaaatatt cctatcgccc
      121 ggccgaaggc aaattcaacg acgaaaagcc ggagtttggc ccggccgaag tgcgaacctt
      181 tgctgaacag gtcaagcacg tcgcctgctc aaactttgcg tttgcggcgg agctggacgg
      241 acagaagccg cctgatgctt gcgacaaagg cgggccgagt ccggcgcgaa ccaaaaagga
      301 actgttgatc tacctgcgcg attcatttgc cgcgattcgc aagtcgttcg gcgtaatcac
      361 cgcgcaaaat atgtatgaac cgattgaagg tccatacgcc gggccgaata cgcggctggg
      421 tttggcgacc gtggttattt ggcacgctgc cgatcactac gggcagatga ctctgtacct
      481 gaggctgaac ggaatcgtgc cgccagcaag ccgtccgaat ccgccggaag tcaaagacag
      541 ctattaattg ttatcaaatt tagcgtaacc ataccgggtc gcgcggcgga aaaacttcca
      601 caagactgca caaaccactt gactatgtcc gcacacggga gcagtgtcac ttccctgccc
      661 gcctggaata cccctctctc gaaaggagta ccccatgccg cggtttcttc accaagtcag
      721 ttacacctcg gaagccatgt cgcgcttgat tgcgaatcca caggaccgct ttgaagcggt
      781 gcgcgggcct gtcgagaagc tcggtggcaa aatcgaacac ggttattttg cgtttggcga
      841 gcacgacgct atcctgatta ccgatatgcc ggacaatatt tccgccgccg cgatcgcgct
      901 ggcgtttgcc gccggcggcg ccctgcgaaa ttgcaccacc actcccctgc tcaccactgc
      961 cgaagcattg gacgcgatgc gtaaggccgc tgcatgcggc tataaaccga tgcaggctgc
     1021 ggccggtgcc gccagccgtt aagcgtcccc aaagaggggt gcggatgtaa cctcgccgcg
     1081 cccctctctt tatgtctgaa ccccgcgagt ggttcgcccg gccgctccct tttgttttgc
     1141 tccccaggct ctttcgaccg ctcatttggt aagatttgcg catgcccgga gaaccgcgaa
     1201 ccgcctggct ggtgaacatc cgtctgcatc ctattaaggg acttgatccg gtcagcgtgc
     1261 cggaagcgcg cattggcccc aacggcggcc tgcagctgga tcgcgtgtgg gtgctttttt
     1321 cggtggatgg caagtggatc aacggaaaaa acaatccgga gattcatcgt attcgcgcgg
     1381 aatacgatcc gctgctgggt agcgtgttgc tcagcgttcc ggttgcagga aattccgaca
     1441 acgctcgcgg tactctaaaa tctgcgcggc ttgcgtttcc ttcggacaca gaacgcgccg
     1501 ccgcctggtt ctcggaatac ttcagccgca cggtcatcgt ccggcatatt cccgaaggcg
     1561 cgcccgacga tgggctggcc actgggccga cgatcgtctc taccgcttcc ctgcaggcgg
     1621 tgtgcgatct ctgtcccggt gtggaattga atgaagcacg cgagcgattt cgcaccacgc
     1681 tcgaaattga cggcgtgccg gcgttctggg aagaccagct cttcggcgaa gacgaaaatt
     1741 atccggtgcg cttcaagatt ggagaagtta ggttcgaggg cagcaatcct tgtgcgcggt
     1801 gtcccgtgcc gccgcgcaat ccgcgaacgg gtgaagacct catcggcttc cagaaaaagt
     1861 tcaccgatat gcgccgatcg cagctgccac atacttcacc acgcgaacgt ttcgaccact
     1921 tctatcgcct ggccactaat acccgcgtcg cctcgaccga acaaggcaag gttctccgcg
     1981 tcggcgacgc attggtgctg taaggcgcta aagccttgag aactttgcct tccacctgag
     2041 tgattcgcgg gactgtcaag cggcctcgct aaaatacttg cgcgggggga acatgccgcg
     2101 acgtaggagt tccttgccgc agcgctggcg cacacctcca cgcgcgacag tgggagcttt
     2161 cgccgacctg cgg
//
LOCUS       DASTPM010000334         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_357826, whole genome shotgun sequence.
ACCESSION   DASTPM010000334 DASTPM010000000
VERSION     DASTPM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357826"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFR42_05870"
     CDS             complement(<1..305)
                     /locus_tag="VFR42_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368714.1"
                     /translation="MKPNKYIAVFSLLNALAFCLTHSSANAGQGDKGSQRGGQASSHM
                     SSKGSANSNAQWSADPERGWVRADERQQRHEESHETSKAKKNGGKHNAKGAREKNND"
     gene            complement(335..478)
                     /locus_tag="VFR42_05875"
     CDS             complement(335..478)
                     /locus_tag="VFR42_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368715.1"
                     /translation="MKSPNYVAVLAILNAILHRVSESLPLIDQATRELGNSKIPAKAR
                     AAE"
     gene            complement(622..1977)
                     /locus_tag="VFR42_05880"
     CDS             complement(622..1977)
                     /locus_tag="VFR42_05880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0368716.1"
                     /translation="MARPRAKVLVVDDDPATRDGLTALLETWGHSASSAADGKAALKL
                     CEKELPHAIVTDLMMPGMTGLEFIEGLGERVRQVAVVMMTGQATIETAVQAIKLGAYD
                     YLTKPLEPEKLRAVLEKGLKQVSLTREASVLRQKLESPLGSYGALIGKSAPMRQLYQH
                     IGQIAATDAAVLVNGESGTGKELVARTLHDLSPRREGTFLALNCAAISPTLMESELFG
                     HEKGAFTNAIERHLGCFEQADGGTLFLDEITEMAAEVQAKFLRVLEEGQVRRIGGTAN
                     IPVSVRVVAATNRSPAPAVKEGKLREDLFYRLNVFNLELPPLRERGEDIALLARYFLE
                     SFAEKYRRPLLQWATDFAGALQSHPWPGNVRELRNAMERLAVLAPGPSLTAADVQQFC
                     LAAVVEDLSDAAPLSLAEAERQAIERALAASGGNKTQAARILGITPKTLNAKLSLYNK
                     E"
     gene            complement(1980..3506)
                     /locus_tag="VFR42_05885"
     CDS             complement(1980..3506)
                     /locus_tag="VFR42_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0368717.1"
                     /translation="MSLRQQQIAVFFGTVVVIFSIFAGGVWLIAQRLTREATLQTAVL
                     MARQVEIALADSLRQRPAPQGRIVQSQPSSSFWSFLGNIFPGVGSKPKTQYIPGRPAS
                     RQAEVKGLMEAFVNRSGSIEAMWVLNADGKILYSSMGHEKGEALTDPNLRNILRRGVS
                     TINAQRQGKSTYYDVLVPLQMPAGVEGPGGLRLWINPADWTEMLSGLWRQLTLLFALG
                     GGVALLSAFLTTALYTRRFRLISETLRQAEAGTYQTRPQYRSHDEVGTSLDLIDRLVM
                     KQRKTVGAPAPMQRLAYAARTLAHEVRTPLNALAIHLELLRNSGNRPDDQQQKSIGAL
                     DASVRQVDRLVREFSDYSAPVTMERKTTDVFEVLATSLEAAAAQCAPKNISLKKDLVP
                     GPWLIQGDGTRLRQCFDNLLRNAMEAQPDGGSIRVTASKNTKELVLQFADSGPGVAPE
                     HRAELFEFGKTTKVGGSGIGLPLSQLIVESHGGTLVYEDQNDASSGASFRVTLSLEGN
                     "
BASE COUNT          736 a          993 c         1014 g          808 t
ORIGIN      
        1 tggtcgttgt tcttctctct tgcgccttta gcgttgtgct tgccgccatt tttcttagcc
       61 ttgcttgtct cgtgagactc ttcgtgccgt tgttggcgtt cgtccgcgcg tacccagcct
      121 cgttcggggt ctgccgacca ctgggcattt gagttagcgg atcctttgct gctcatatga
      181 gaactcgctt gaccgccgcg ctgcgagcct ttgtcgccct gtccagcatt tgccgatgaa
      241 tgtgtcaaac aaaacgccaa cgcgtttagg agtgaaaaga cagcaatata tttgttcggc
      301 ttcataaaat ctccgcttgg ttaagcgcgc aacttcactc ggcggcgcga gccttcgcgg
      361 gaatcttact gttccccaac tctcgtgtag cttgatcgat cagaggcagt gactcggata
      421 cgcgatgtaa tatcgcgttg agaatggcca agacggcaac ataattaggt gatttcattt
      481 ttcctccctt gggacgtatc gcccagttgc aagtcaggtt gagcaacgct tatgccagtt
      541 ggaaaggcgt gtaaaatcag cgtcttatgt aggaccaaga cgcaaccggg gcaagtaaaa
      601 aggtagccct acaaggagaa attactcttt gttataaagg gacaatttgg cgttcagggt
      661 ttttggggtg atgcctagaa ttcgtgcggc ttgagtcttg ttgccaccac tggctgccaa
      721 ggccctttca atggcctgcc gctcggcttc ggctagtgaa agcggtgccg cgtcggatag
      781 gtcttccacc accgcagcaa gacaaaattg ttgcacatcg gctgcggtca ggctcggacc
      841 gggcgccagt actgcgagtc gttccatggc gttgcgcagc tcgcggacat tgcctggcca
      901 cgggtgggat tggagagcgc ccgcgaagtc agtggcccat tgcaataagg ggcgccggta
      961 tttctcggcg aaggattcaa ggaaataccg tgccagcagc gcaatgtctt cgccgcgttc
     1021 ccgtagtggc ggcaactcca aattgaaaac attcaaacga taaaagagat cttcccgcag
     1081 ttttccctct ttgacggcgg gcgcggggga acggttggtg gcggcgacga ctcgcactga
     1141 taccggtata ttagcagtgc caccgatacg gcgcacctgt ccttcctcta aaacgcgcaa
     1201 aaacttcgcc tggacttcgg ccgccatctc agtaatctcg tcgagaaaga gcgttccacc
     1261 gtcagcctgc tcgaagcaac ccaagtggcg ctcgatggcg ttggtgaagg ctcccttctc
     1321 gtgaccaaaa agttcacttt ccatgagggt tggggaaata gccgcgcagt taagcgcgag
     1381 aaaagttccc tcgcgccgcg gtgaaaggtc atggagcgtg cgcgcgacca attctttgcc
     1441 agtgccgctt tcaccgttga ctaacacagc ggcgtccgtt gctgcgattt gaccgatatg
     1501 ctggtaaagc tggcgcatcg gggccgattt tccgatcaat gctccatagg agcccagcgg
     1561 cgattccaac ttttgacgca aaacactcgc ttcacgcgtc agcgaaactt gtttgagtcc
     1621 cttttcgagc acggctcgca acttctccgg ctcgagcggc ttggtgagat aatcataggc
     1681 accgagcttg atggcttgaa ccgcagtttc gatggtcgcc tggcccgtca tcatgacgac
     1741 agcgacctgc cgcactcgtt caccgaggcc ttcaataaat tcgagcccgg tcatgcccgg
     1801 catcatcaga tcggtcacga tagcatgcgg cagttccttt tcgcagagct taagagccgc
     1861 tttgccatcg gccgctgagc tggcgctatg gccccaggtt tccaatagcg ccgttagccc
     1921 gtcacgggtg gcgggatcgt cgtcaacgac gagaaccttt gcacgagggc gggccataat
     1981 tagttcccct ccaacgatag agtcacacga aagctggctc ctgagcttgc gtcgttttga
     2041 tcttcgtaaa ccagtgtacc gccatgggac tcaacgatca gttggctcag cggtagtccg
     2101 atgccgctac cgccgacctt cgtcgtcttg ccgaactcaa acagctccgc gcggtgctcc
     2161 ggcgcgacgc cgggaccgga gtccgcgaac tgtagaacca gttccttggt gttcttgctg
     2221 gcggtaacac gaatgctgcc gccatccggt tgcgcttcca tggcgttgcg caagaggttg
     2281 tcaaaacact ggcgcagacg cgtgccgtcg ccttgaataa gccacggacc cggtaccaga
     2341 tcttttttta gagaaatatt cttcggtgcg cattgtgccg ccgccgcttc gaggcttgtt
     2401 gccagcactt cgaacacatc cgtcgttttc ctttccatgg taaccggcgc ggaatagtcg
     2461 ctaaactcgc gtacgagccg atcgacttgc cggacgctgg catcgagcgc gccgatggat
     2521 ttttgctgct ggtcatccgg gcggttgccg gagttgcgga gcagttcgag atggatcgcc
     2581 aatgcgttca aaggggtgcg tacttcgtgc gccaaggttc gcgcggcgta agccaggcgc
     2641 tgcatgggcg cgggcgcgcc aacggttttg cgttgcttca tgaccagacg atcaatcaaa
     2701 tccaaactcg ttcccacttc gtcgtgactc cggtactggg ggcgggtctg ataggtgccg
     2761 gcttcagctt ggcgcaaggt ttcgctaatc aaacgaaaac ggcgcgtgta gagagccgtg
     2821 gtcaggaacg cactcagcaa agccacgccg ccaccgaggg cgaataacaa tgtaagttgg
     2881 cgccatagac ccgagagcat ctccgtccag tcggccggat tgatccaaag cctgagaccg
     2941 cccggtccct ctacgccggc gggcatttgc agtggcacca ggacgtcgta gtaagtcgat
     3001 tttccctggc gttgggcatt gatggtggaa acaccacggc ggagaatgtt tctaagattt
     3061 gggtcggtga gggcttcgcc cttttcgtgg cccattgaac tgtagagaat cttaccgtcg
     3121 gcattgagta cccacatcgc ttcgatactg ccgctgcggt taacgaatgc ctccatcaat
     3181 cctttgactt cagcttgccg cgaggccggg cgtccaggaa tatattgggt tttaggtttc
     3241 gagccgacgc cgggaaaaat gttacccaag aagctccaaa aggaggaaga gggctgtgac
     3301 tggacaatac gcccttgggg tgcgggacgc tggcgcaggc tgtcggccaa agcgatctcg
     3361 acctgacgcg ccattaagac ggctgtttgg agcgttgcct ctctagtcaa gcgctgggcg
     3421 atgagccaaa cgcccccggc aaaaatactg aaaatcacca cgaccgtgcc gaaaaagact
     3481 gcgatctgct gctggcgcag gctcattgcc gcgataggat aacagatccc cctgaaccag
     3541 caaagttgaa a
//
LOCUS       DASTPM010000335         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_415707, whole genome shotgun sequence.
ACCESSION   DASTPM010000335 DASTPM010000000
VERSION     DASTPM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415707"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFR42_05890"
     CDS             complement(<1..216)
                     /locus_tag="VFR42_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368718.1"
                     /translation="MSSTQMLPLDPEKRSTDTMEFQTALRSKIVGQEEGVQALVDLYQ
                     VFCAGLNSPGRPVGNLLFLGPTGSGKTR"
     gene            complement(213..665)
                     /locus_tag="VFR42_05895"
     CDS             complement(213..665)
                     /locus_tag="VFR42_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368719.1"
                     /translation="MAFVRRKGNAYYLVHNVRRGGKVRQLHLARLGDRAKITDTIVRE
                     VSKKHPFVELNWRALRAQLSRQINLADPQSPEVQKLIANLRALHLDLADLFPPVLQIS
                     ESPAVAKELLVQLQLLYSTIQVKLEQFRPGRGRLGIASLQPRDARRRS"
     gene            1026..1373
                     /locus_tag="VFR42_05900"
     CDS             1026..1373
                     /locus_tag="VFR42_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU0368720.1"
                     /translation="MSWSPVYALVKLIPRGRVITYGGLAKALSLRGGARSAGRAMAAS
                     PSGKGIPWHRVVGFGGKLLIREPYASLQRKLLESEGVEMTERRVNMKLHAWAPAKKAK
                     SKGISKKRFSRKP"
     gene            complement(1398..>2201)
                     /locus_tag="VFR42_05905"
     CDS             complement(1398..>2201)
                     /locus_tag="VFR42_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368721.1"
                     /translation="GRYHGFYREQLQICVNFATAPLALLGLWFYFVKQDGKRCRALGW
                     LFVVAFLLFALFGARSYYTAPLYPMLLAAGSVLFASWAASLGPLWSKVVYATQWTAIV
                     FGGGAFILLVTPVAPFGSAVWKVSSKMHDQFREEIGWPDLAHTVAQVYISLSPEERAQ
                     AGILTGNYGEGGALNLYGPALGLPPAMTLTNSFWYRTFDQRQPQTVIVVGFDFDEAQK
                     IFASCAIAAKNANAGKVENEESRDHPDILVCRSLKMHWPEYWANHRRFG"
BASE COUNT          485 a          644 c          592 g          480 t
ORIGIN      
        1 gcgcgttttg cccgaaccgg tcggccccaa aaagagcaga tttcctaccg gacgccccgg
       61 cgaattcagc cccgcgcaaa acacctggta caaatccacc agcgcctgca caccctcttc
      121 ctgccccacg atcttcgacc gtagcgccgt ctggaattcc atggtgtcgg tacttcgttt
      181 ttctggatcc agcggaagca tttgtgtgct gctcatgacc gcctcctcgc gtcgcgcggc
      241 tgcaacgacg caattcccaa acgtccgcgt cccggcctga attgttccaa tttgacctgg
      301 atcgtggagt acaggagctg caattgtacg agcaactcct tagccactgc gggcgattcg
      361 gaaatttgga gcacgggcgg aaacaagtcc gccaaatcaa gatgcagtgc tcgaagattt
      421 gcgatgagtt tttgcacttc cggcgactgg ggatcggcca ggttgatctg ccggctcaac
      481 tgtgcgcgca acgcgcgcca attcaattcc acaaagggat gtttttttga cacttcccgg
      541 acgatggtgt ccgtaatttt tgcgcgatcc cctaaccgtg ccaggtgcag ttgccgcact
      601 ttcccgccgc ggcgcacatt gtgcaccaag tagtaggcgt ttcctttgcg gcgaacaaaa
      661 gccatacttc acctttaact cccacttctg cgcgaaccac ccaactgcca aatgtgcccg
      721 tgtggcgcgt agtgtatgga ccttccacta cggcgtcaac aagttagatg ccctaagccc
      781 tcaaaaagtg tcctaaaagt gacactcagc aacttaacgt cttttccttg cgatttctca
      841 tcactttttc cgctgcgttc catttgtttc cctaatgatg tttacgttgt tcagcgtact
      901 taagttttac gaatttgccc ttcctttata tccaacacgc cgttcgccaa ttccactctt
      961 gcacgcacac tactagcact tctagcatgc ttcgtgtcgc actggctcgc ggaggcggcc
     1021 ctggaatgtc ctggtcaccg gtttacgcgt tggtgaaact aattccgcgc gggcgggtta
     1081 ttacctatgg agggcttgca aaggctctga gtctccgcgg aggagcgcgc agcgctggcc
     1141 gagcgatggc cgcaagccct tccggtaagg gcattccctg gcatcgcgtg gtgggtttcg
     1201 gcgggaaact actgattcgc gagccatatg ccagcctgca gcgcaaattg ctggaaagcg
     1261 aaggcgtaga gatgacggag cgccgcgtga acatgaaact gcacgcctgg gcgcctgcga
     1321 aaaaagcgaa gtctaaagga atctcgaaaa agcgcttttc ccgaaagcct taggttttct
     1381 ttttccagac tagtaggtca gccgaagcgg cgatggtttg cccagtattc gggccagtgc
     1441 attttcaagc ttctgcacac gagaatgtcg gggtggtcgc gggattcctc gttttcaacc
     1501 ttcccggcgt tggcattttt cgccgcgatt gcgcaactcg caaaaatctt ctgcgcttcg
     1561 tcgaagtcaa aaccgacgac aatcaccgtc tgcggctgcc gctgatcaaa ggtgcgatac
     1621 cagaaagaat tggtgagcgt catcgctgga gggagcccca gcgccggtcc gtaaagattc
     1681 agagcgccgc cttcgccgta attccccgtg aggattcccg cctgtgcgcg ctcctccggg
     1741 gaaagtgaaa tgtaaacctg agcaacggtg tgtgccagat cgggccagcc gatttcttcg
     1801 cggaactggt cgtgcatttt gctggacacc ttccagactg cagaaccgaa cggagcgacc
     1861 ggcgtgacca gaagaatgaa tgcgccgccg ccgaagacaa tcgcggtcca ttgcgtggcg
     1921 tacactactt tcgaccacag tgggccgaga cttgccgccc agcttgcgaa gagaacgctt
     1981 ccagcagcga gaagcatggg gtaaagcggc gcggtgtaat aggagcgcgc gccgaacagc
     2041 gcgaagagca agaaagccac cacaaacagc cagcccagcg cgcggcagcg cttcccgtcc
     2101 tgcttgacga aataaaacca caaaccaagc agcgcgagcg gagcggtcgc gaagttcacg
     2161 caaatttgca attgttcacg ataaaagccg tgataacggc c
//
LOCUS       DASTPM010000336         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1194060, whole genome shotgun sequence.
ACCESSION   DASTPM010000336 DASTPM010000000
VERSION     DASTPM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194060"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFR42_05910"
     CDS             <1..584
                     /locus_tag="VFR42_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368722.1"
                     /translation="SSFNQKHKFTGNWMYDLPFGENHRLASKGALSHILNGWQWSGSA
                     TIGSGFYFTPNVLGGAVDISRGVSGSQRANYVPGQSISISNPTSLAWFNTAAFCTSTT
                     SAACSNPSGSAFGDAGRFIIEGPGQFSLNMALSKTIQIRETRALELRLQANNIFNNVV
                     FSGLNTTVNSLAFGEVTSVGSMRKITMIARFRF"
     gene            600..>2678
                     /locus_tag="VFR42_05915"
     CDS             600..>2678
                     /locus_tag="VFR42_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0368723.1"
                     /translation="MLERFIRMVCACALLTQFPLAALAQQTTHQQSAPAIRATTELVL
                     VNVVARDKKGNLVRDLKRQDFTVLEDGQKQQLASFDFENIDELALAQQRMTTTNGASA
                     PVAASAPAQPAEAASDARDRRLILLFFDFSAMDPEQIDRAVDAAKKYVNTKMQSADLI
                     AIVSLTTNMRLDLNFTDDKAKILSTLSAYNSSSGQGFDNGATGSGEGSAETGGAFTAD
                     DTDYNTFSADRKLLALQSIIQSVGKLSQKKSLIYFSNGISQSGVDNQSALRAATAAAV
                     KANVAIYPLDVRGLQALPAGGEAQNASLHGQSAYSGASVLNDLNSNASTQETLSTLAA
                     DTGGKAFFDSNDFSGVFSQVQKDTSAYYILGYSSSNHVKDGRFRRIKVVVNRPDLKLE
                     YRAGYYAGRDFEHSGRADREAQLQDELASDLPATDVAVYAGTAYFRRDDSHYFLAVSI
                     VVPGSQIPFVQEKDRDSATLDMAGVVLEGGKFQVGQLRDTVKLAVDSTQQVRRKNVQY
                     NTSFLLAPGSYHLKFVVRENRTGRMGTFETDVQIPDLRKVPLRSSSVVLSSLRVPATG
                     KNKATNPLVGDQTELVPNVTHVFTQDQHLYLQFEVYDAAKSKSLPTRKPAGAAPASGP
                     PPDRPAPKSPGETIRVLTSVQFMQNDVKVYESKPIIATEVNVPQRKAVEFQLDLPLQT
                     LKPGFYTCQVN"
BASE COUNT          599 a          933 c          652 g          494 t
ORIGIN      
        1 tttccagctt caatcaaaag cacaaattca ccggcaactg gatgtacgac ctcccgttcg
       61 gcgaaaatca ccgcctcgcc tccaagggcg cgctctcgca cattttgaat ggctggcagt
      121 ggagcggcag cgccaccatc ggctctggct tctatttcac acccaacgtc ctcggcggcg
      181 ccgtggacat cagccgcggc gtaagcggct cgcagcgcgc caattatgtg cccggccaat
      241 cgatctccat cagcaacccg accagccttg cgtggtttaa caccgcggcg ttttgtacgt
      301 ccaccacctc agcggcctgc agcaatccca gtggctccgc attcggcgac gcgggaagat
      361 ttatcatcga ggggcctggc caattttcac tcaacatggc gctcagcaag accatccaga
      421 ttcgcgaaac tcgcgcgctc gaattgcgcc tgcaagccaa caacattttt aacaacgtcg
      481 ttttttcggg actcaacacc acggtaaatt cgctggcctt tggcgaagtt acctcggtcg
      541 gctccatgcg caagatcacc atgatcgcgc ggtttcgatt ttaagaaagg ggaagcggca
      601 tgttggaaag attcatccgt atggtttgcg cttgcgcgtt gctcacgcag tttcccctcg
      661 ccgcgctggc ccagcaaacg acgcaccagc aatccgcgcc cgccatccgt gccaccaccg
      721 aactcgtcct tgtcaacgta gttgcgcgcg ataaaaaagg taacctggtc cgcgatctga
      781 aacgacaaga cttcacggtt ctggaagacg gccagaagca gcagctcgcc agcttcgact
      841 ttgaaaatat tgacgaactc gcgctcgcgc agcagcgcat gaccaccaca aacggcgcgt
      901 ctgctcccgt agccgcctca gccccagcgc aaccagccga agccgcatcc gatgcccgcg
      961 accgccgcct catccttctc ttcttcgatt tctccgccat ggaccccgaa caaattgacc
     1021 gcgccgtcga tgccgccaag aaatacgtca acaccaaaat gcaaagcgcc gacctgatcg
     1081 cgatcgtgtc gctaacgacc aacatgcgct tggatttgaa cttcaccgac gacaaggcaa
     1141 aaatactttc caccctgagc gcctacaatt ccagctccgg ccagggcttc gacaacggcg
     1201 ccaccggcag cggggaaggc tcggcggaaa ccggcggcgc gttcaccgcc gacgataccg
     1261 actacaacac tttcagcgcc gaccgcaaac tcctcgcgct ccagtccatc atccagtccg
     1321 tggggaaact ttcgcaaaaa aagagcctca tctatttcag caatggcatc agccagtctg
     1381 gtgtggacaa tcaatccgct ctgcgcgccg ccaccgccgc agccgtcaaa gccaacgtcg
     1441 ccatctaccc gctcgatgtt cgcggactcc aggccctgcc cgccggtggc gaagcacaaa
     1501 acgccagcct ccacggccag tcggcctatt ccggcgcgtc ggtcctcaac gacctcaaca
     1561 gcaacgcctc cacacaggaa acgctttcca ctctggccgc cgacaccggc ggcaaggctt
     1621 ttttcgattc caacgatttc agcggcgtct tttcccaggt gcagaaagac acttcggcct
     1681 actacattct cggctactcc agttccaatc atgtgaagga cggccgcttc cgccgcatca
     1741 aggtcgtcgt caatcgcccc gacctcaagc tggaatatcg cgcgggctat tatgccggcc
     1801 gcgattttga acactctggc cgcgccgatc gcgaagccca gctgcaggac gagcttgcct
     1861 cggacctgcc cgccaccgac gttgccgtct acgcgggcac cgcctatttc cgccgggatg
     1921 attcgcacta tttcctcgcc gtttccatcg tcgtgcccgg ctcgcagatc cccttcgtcc
     1981 aggaaaaaga ccgcgacagc gctactctgg atatggccgg cgtggtcctc gaaggcggca
     2041 aattccaagt cggccagtta cgcgacaccg tcaagctcgc cgtagacagc acgcagcagg
     2101 ttcgccgcaa aaatgtgcaa tacaacacca gcttcctgct ggcgcccggc agctatcacc
     2161 tcaaattcgt ggttcgcgaa aatcgcaccg gccgcatggg cacgtttgaa accgatgtac
     2221 agattcccga ccttcgcaaa gtgccgctgc gcagcagctc cgtcgtgctc agcagcttgc
     2281 gcgtccccgc caccggaaaa aacaaggcca caaatccgct cgtcggcgac caaaccgaat
     2341 tagtccccaa cgtcacccac gttttcacgc aggaccagca cctctatttg caatttgaag
     2401 tgtatgacgc ggcgaaaagt aaatccttac ccactcggaa gcccgctggc gcggcaccgg
     2461 catccggacc acctcctgac cggcccgcac ccaagtcgcc aggcgaaacc atccgcgtcc
     2521 tcaccagcgt gcaattcatg caaaacgacg tgaaagtcta cgaatccaag cccatcatcg
     2581 ccaccgaagt gaatgtgccg caacgcaaag ccgtcgagtt ccaactcgac cttccgctgc
     2641 aaaccctcaa gcccggcttc tacacctgcc aagtgaat
//
LOCUS       DASTPM010000337         5171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_876870, whole genome shotgun sequence.
ACCESSION   DASTPM010000337 DASTPM010000000
VERSION     DASTPM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876870"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="VFR42_05920"
     CDS             <1..1907
                     /locus_tag="VFR42_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU0368724.1"
                     /translation="AVKLAMGSLNLEDEELQPRVILSQISFAKNHAKTPGMLRSEAYT
                     PDLRHAADVFAQYEKILAQGKALDFDDLLLRSARLLREAPQVREKWQHRFQYIHVDEY
                     QDTNRVQYDLLRLLVGGHQNICVVGDEDQSIYRWRGADVSILLSFSRDFPNAKVVRLE
                     RNYRSTQSILDAAGAVVKNNSERLGKNLRAEISGGANLRYFEARDAQTEAEFVAEELR
                     KLLDDDSSQTCAVEYRTNFQSRAFEEVFRRHGIRYKLVGGFSFYKRAEVQDTLAYVRL
                     AMHPEDDIAFLRVLNVPPRGIGKTTLESLRDSARKNGTSFWTAMEDFITANATGRAVT
                     PLRAFMDLIASLQAALQEKEPADFIRFVLDETGYMAMLRDRNTPEDVARMENLEELTR
                     AVAESMDAGESFTDFLDAAALVSDADSFEGKPGVTLITLHSTKGLEFDHVFLTGMEEG
                     ICPHSRSINDGDAKAIEEERRLVYVGMTRARKTLTLTRAVYRRVFGNEQQMRASLPSR
                     FLAEIPSELVDTVRGSMSEIGQTRRYEPDPEYSYSAEEFLRRVRRGNAPAVRQPSPPR
                     ARTEPSFGRPARRASSDPLIGQKVRHPEYGVGTIIGVEGDEEDRRLSVTFPGRGTKKF
                     VERYAQLQQV"
     gene            2033..3568
                     /locus_tag="VFR42_05925"
     CDS             2033..3568
                     /locus_tag="VFR42_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0368725.1"
                     /translation="MPSLLFRRKNIDQLISDCEGAELRLKKSLGWLSLTSLGIGAVIG
                     SGIFTIVGTAIAGQKFQTSSILNVPLLQYLVNHSESFGRPGAGPAIALSFVLVAIACG
                     FASVCYAELAAMIPIAGSAYTYTYATMGEIIAWIIGWDLILEYAVSNMAVSVGFSAHL
                     VDLLDWFGVHPDPKWISPAYLPSGLSDLSGNMIYGPGWHFGFNWPAFIIVMLLTVILV
                     RGIRESARTNNIMVLIKIVAILAFITFASRFIHPSNWHPFSPNGWPGILTGGSIVFFT
                     YIGFDSVSTAAEECKSPQRDLPRGIIATLVVCTLLYIGVVIVLTGLVRWDTLLDDAAP
                     VVNTLRKLNISGLRLVVLIGALMGMISSLLVFQIGQARVWFAMSRDGLFPKIFSRVHR
                     SYLTPDFSTWVAGFVVGIPAGLLDIGTFADLSNIGTLFAFALVAAGVLILRYREPNRQ
                     RAFRAPGGPLAPLMTIITCLLLMAGLPIMNWIRFFVWLAIGLVIYFTYSRKRSTLQLQ
                     ATK"
     gene            3580..4419
                     /locus_tag="VFR42_05930"
     CDS             3580..4419
                     /locus_tag="VFR42_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368726.1"
                     /translation="MSPRLRLRFCLALAIALWPSLVLAWGNNANRLVVNRAIDALPQD
                     IRPFFDANRSILLQHVNDPFGTPEKALTPTERHNQFLFLDKYGRFPFDTLPRSYKAAV
                     AKFGKSKLESNGLLPWQIGVYSAKLTDALREGRWEDAKLFAAILANYVAGAHDPFNTT
                     DNNDGHLSAQNGVNERFGTALVDRYSSYFFMRPNDAAFIADPTDHAFEACLSSHSWLE
                     TILYADREARRGLNSYTDEYYDHFYNQAGAVLIRQLSDAASDVGSYWLTAWKNAGQPQ
                     LPR"
     gene            4499..>5171
                     /locus_tag="VFR42_05935"
     CDS             4499..>5171
                     /locus_tag="VFR42_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368727.1"
                     /translation="MTVSIAGSNFLRDLPKAELHLHLEGAIQPGTVCALTARHGISTT
                     EDEVRRRYNYNDFLGFLDAFKWVTSFLRDPADYALITHDLCEQLLQHRVVYAEVTLSV
                     GVMLLRNQSPEKNFAAILRAAEDFASRGLTLRWIFDAVRQFGPDAALRVVQAAKICAA
                     PQVVAFGIGGDELSIPTKDFLPVYEAASNAGLHRLVHAGEIGGPEKIREAVEFLRVER
                     VGHGIA"
BASE COUNT          981 a         1803 c         1285 g         1102 t
ORIGIN      
        1 ccgccgtgaa gctcgccatg ggcagcctca atctcgaaga cgaggagctg cagccccgag
       61 ttatcctcag ccagatcagc tttgcgaaaa atcacgccaa gacgccggga atgctgcgct
      121 ccgaagccta cacgcccgac ctccgccatg ccgccgatgt tttcgcacag tacgaaaaaa
      181 tcctcgcgca aggcaaagcg ctcgacttcg acgatctcct gctccgctcc gcgcgtctgc
      241 ttcgcgaagc gccgcaagtc cgcgaaaaat ggcaacaccg ctttcaatac attcacgtcg
      301 acgaatatca ggacaccaac cgcgtccagt acgatttgct tcgtctgctc gtcggggggc
      361 accagaacat ctgcgtggtt ggcgacgaag accagtccat ctaccgctgg cgcggcgctg
      421 acgtttccat ccttctcagt ttttcgcgcg actttccgaa cgccaaagtg gttcgcctgg
      481 agcgcaatta ccgctctacg caaagcatcc tcgacgcggc cggcgccgtc gtcaaaaaca
      541 attccgagcg cctcggcaaa aatcttcgcg cggaaatctc cggcggagcg aatctgcgct
      601 actttgaagc ccgcgacgcc cagaccgaag ccgaatttgt cgccgaggaa ctccgcaagc
      661 tgctcgatga cgactcttcg caaacctgcg ccgtcgaata ccgcaccaac tttcagtcac
      721 gcgcgttcga agaagtcttc cgccgccacg gcattcgcta caaactggtc ggcggtttca
      781 gcttctacaa acgcgccgaa gtgcaggaca ccctcgccta cgtgcgcctc gccatgcatc
      841 ccgaagacga cattgcattc cttcgcgtgc tcaacgttcc tccgcgcggc atcggcaaaa
      901 ccacactgga atctctccgc gactccgcgc gcaagaacgg cacctcgttc tggaccgcga
      961 tggaagattt catcaccgcc aacgccaccg ggcgcgcggt caccccgctg cgcgctttca
     1021 tggacttgat cgccagcctg caagccgctc ttcaggaaaa agagcctgcc gacttcatcc
     1081 gcttcgtcct cgacgagact ggctacatgg ccatgctccg tgaccgcaac actccggaag
     1141 acgtggcccg catggaaaat ctcgaagaac taacccgcgc tgtcgcggaa agcatggacg
     1201 ctggcgaatc cttcaccgat tttctggacg ccgcggcgct cgtaagcgat gccgactcgt
     1261 ttgaaggcaa acccggcgtc acgctcatca ccctacacag caccaagggc ctcgaattcg
     1321 atcatgtttt tctcaccggc atggaagaag gcatctgccc gcacagccgc tccatcaatg
     1381 atggcgatgc caaggccatt gaagaggagc gccgcctggt ttatgtgggc atgacccgcg
     1441 cccgcaaaac gctcacgctc acacgcgctg tctaccgccg cgttttcggc aacgaacagc
     1501 agatgcgcgc ttccttgccc tctcgttttc tcgccgaaat tcccagcgaa ctggtcgaca
     1561 ccgttcgcgg ctccatgtcc gaaatcggcc aaacccgccg ctacgagccc gatcccgaat
     1621 actcctattc cgccgaagaa tttctgcgcc gcgttcgtcg cggcaacgct ccggccgtac
     1681 gccagccgtc gccgccgcgc gcgcgtaccg agcccagctt cggtcgccca gcacgccgcg
     1741 catcctcgga tccactgatc ggccagaaag ttcgccatcc cgaatacggc gtcggcacca
     1801 tcatcggcgt cgaaggcgac gaagaagatc gccgtctgtc ggtcaccttc cccggtcgtg
     1861 gcaccaagaa attcgtggaa cgctacgccc agctccagca agtctaatca tcccacctgc
     1921 cccggatcgt cattccgagc gtagcgagga atctctcttc gatcgttacc aggctcggtt
     1981 gcgccttcgc cgccactcac gataaagtgt ggctcgcacc ttggagacac gagtgccctc
     2041 actcctcttc cgtcgcaaga acattgatca gctcatttcc gattgcgaag gcgccgaact
     2101 tcgcctgaaa aagtctctgg gctggctgag tctcacatcc ctcggcatcg gcgcggtcat
     2161 cgggtcgggc atctttacca tcgtcggcac cgccatcgcc gggcaaaaat ttcaaacctc
     2221 ttcgatcctg aacgtcccgc tcctccaata cctcgtcaat cattccgagt cttttggccg
     2281 gcccggcgcc ggtccagcga tcgcgctctc gtttgtcctt gtcgccattg cctgcgggtt
     2341 cgcttcggtc tgctacgccg aactcgccgc catgatcccg atcgccggca gcgcctacac
     2401 ctatacgtat gccaccatgg gcgaaatcat cgcctggatc atcggctggg acctcatcct
     2461 cgaatacgcg gtcagcaaca tggccgtcag cgtcggcttt tccgctcatc tcgtcgatct
     2521 actcgactgg ttcggcgttc atcctgaccc caaatggatt tctcccgctt atcttcccag
     2581 tggcctcagc gatctttccg gcaacatgat ctacggcccc ggctggcact tcggcttcaa
     2641 ttggccggcg ttcatcatcg tcatgctgct caccgtgatt ctggtccgcg gcattcgcga
     2701 atccgccagg accaacaaca tcatggtgct gatcaagatc gtcgccatcc tggcgttcat
     2761 caccttcgcg tcgcgcttca ttcatccttc caactggcat cccttctcgc ccaacggctg
     2821 gcccggcatt ctcaccggcg gctccatcgt gttcttcacg tacatcggct tcgactccgt
     2881 ctccaccgcc gccgaagaat gcaaaagccc gcagcgcgac ttgccgcgcg gcatcatcgc
     2941 cacgctcgtc gtctgcacgc tgctctacat cggtgtggtc atagtcctca ccggcctggt
     3001 tcgctgggac actttgctgg atgacgccgc ccccgtcgtc aacaccctca ggaagctcaa
     3061 catctccggc ttgcgtctgg tcgtgctgat cggcgctctg atgggcatga tttcttcgct
     3121 gttggttttc cagatcggcc aggcccgcgt atggttcgcc atgtcccgcg acggcctctt
     3181 cccaaaaatt ttcagccgcg tccatcgcag ctacctcacg ccggatttct ctacctgggt
     3241 cgccggcttt gtcgttggca ttccagccgg ccttctggac atcggcacct tcgccgacct
     3301 ttccaacatt ggtacgctct tcgccttcgc gctcgtcgct gccggcgtat taatccttcg
     3361 ctaccgcgag cctaatcgcc agcgcgcctt ccgcgccccc ggcggcccgc tggcgcccct
     3421 catgaccatc atcacctgcc tgctgttgat ggcgggcttg ccgatcatga actggattcg
     3481 tttctttgta tggctggcca tcggacttgt gatttacttc acctatagcc ggaagcgcag
     3541 cacgcttcag ctgcaagcaa cgaagtaaag gcggctcgaa tgtcccccag attgcggctc
     3601 cgtttctgtc tggcattggc catcgcgttg tggccatcgc tcgttttggc ctggggcaac
     3661 aacgccaatc ggctggtcgt caaccgcgcg attgatgctc tgccacagga tatccgcccg
     3721 ttttttgacg ccaaccgttc catcctgttg cagcacgtca acgatccttt tggcactccc
     3781 gaaaaagcgc tcactccaac cgagcgccac aatcaattcc tctttctcga taagtacggt
     3841 cgctttccgt tcgatactct tccccgcagc tacaaagccg cggtcgccaa attcggcaag
     3901 tccaagctgg aatcgaacgg cctgctaccc tggcagatcg gcgtctacag cgccaagcta
     3961 acggacgccc tgcgcgaagg cagatgggaa gatgccaaac tgtttgccgc cattctggcc
     4021 aactatgtag ccggggcgca cgatcccttc aacaccactg acaataacga tggccatctc
     4081 agcgcgcaga acggcgttaa cgaacgcttt ggcaccgccc tggtggaccg ctactcctct
     4141 tattttttca tgcgcccgaa tgacgccgcc ttcattgccg atcccaccga ccacgctttc
     4201 gaagcgtgct taagctccca tagctggctc gaaaccatcc tttacgccga ccgcgaagcc
     4261 cgccgcggcc tgaactccta cactgacgaa tactacgacc acttttacaa tcaagcgggc
     4321 gctgtcctga ttcgtcagct atccgacgcc gcctccgacg tcggttcgta ctggctcacc
     4381 gcctggaaaa acgccggcca gcctcaactc cctcgctgaa tttctctgcc tctccgcgag
     4441 cctgcgcgca ctctgcgcct cggcgttttc ttttttccgt tcctgcgcct gataaactgt
     4501 gacggtgtca atcgccggat caaatttcct tcgcgatctt cccaaagctg aattgcacct
     4561 gcatttggaa ggcgctatcc agcccggaac agtctgcgcc ctcacggctc gccacggtat
     4621 cagcaccacc gaagacgaag tccgacgtcg ttacaactac aacgatttcc tcggctttct
     4681 cgacgccttc aaatgggtca cgtccttttt gcgcgatccc gctgactacg cgctgatcac
     4741 gcatgatcta tgcgaacagc tcctgcagca tcgcgttgtc tacgcagagg tcacgctctc
     4801 ggttggcgtc atgcttctcc gcaaccagtc gccggaaaaa aacttcgctg ccatcctccg
     4861 cgccgccgaa gattttgcat cgcgaggact cacactacga tggattttcg acgcggtccg
     4921 tcagttcggc cccgatgccg cgctgcgtgt cgtccaagcc gcaaaaatct gcgccgcgcc
     4981 gcaagtcgtc gcgttcggca tcggcggcga cgagctcagc atccccacca aagattttct
     5041 tcccgtttac gaagctgcct ccaacgccgg actgcatcgg cttgttcacg ccggcgaaat
     5101 cggcggccca gaaaaaattc gcgaagccgt ggagtttctc cgcgtggagc gcgtcggcca
     5161 cggtattgcc g
//
LOCUS       DASTPM010000338         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1222815, whole genome shotgun sequence.
ACCESSION   DASTPM010000338 DASTPM010000000
VERSION     DASTPM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222815"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VFR42_05940"
     CDS             complement(<1..936)
                     /locus_tag="VFR42_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0368728.1"
                     /translation="MCDLVKLRQSWTSAAFLTSCVMSLLISTVQSPAQTTVRMGHFPN
                     ITHVQALVAHNMSRQGKGWFEQRLGPDVKIEWYVYNAGPSAMEAIFAKSIDVTYVGPN
                     PAINAYVRSRGQEIRIVAGSVNGGSALVVHKGAALKTPVDFRGKKIATPQFGNTQDVA
                     ARAWLSSGGLKVTQAGGDAQVLPTANPDQLLLFKQNQVDAVWTVEPWVSRLEQEADGE
                     VLVDDREAITTVLVARADLLATQQGLVRKLVNAHRELTDWIIKNPAEAERMAREELDE
                     EMHTKFSPALIARAWSRIVVTSDLTLDSLQKFVSSA"
     gene            1381..2145
                     /gene="cysD"
                     /locus_tag="VFR42_05945"
                     /pseudo
     CDS             1381..2145
                     /gene="cysD"
                     /locus_tag="VFR42_05945"
                     /inference="COORDINATES: protein motif:HMM:NF003587.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
BASE COUNT          466 a          648 c          587 g          475 t
ORIGIN      
        1 cgcgcttgac acgaatttct gaagcgaatc gagcgtcaaa tcactggtca ccacgatcct
       61 gctccaggcc ctcgcaataa gtgcgggcga gaatttcgtg tgcatctcct cgtccagttc
      121 ctcacgagcc atgcgttcgg cctcagccgg gttcttgatg atccaatcgg tcagctcgcg
      181 gtgcgcattg accaatttac gcaccaagcc ttgttgggtt gcaagcaggt cggccctggc
      241 cacaagaacg gtggtaatgg cctcccggtc atcgacgaga acctcgccat ccgcctcttg
      301 ttccagccgg gacacccatg gctcgaccgt ccagaccgca tcgacttgat tttgcttgaa
      361 tagcagcagc tggtccggat tggcggtcgg caggacctgc gcgtcgccgc ctgcctgcgt
      421 cactttgagg ccgccggatg aaagccaggc gcgagccgcg acatcctggg tgttgccgaa
      481 ttgcggagtg gcgattttct tgccgcggaa atcgaccggt gtcttcagcg cggctccctt
      541 gtgcacgacc agtgcggagc cgccattcac ggagccggcc acaatgcgga tttcctggcc
      601 gcgcgagcgc acgtaggcgt tgattgcagg gttgggcccg acataggtca cgtcgattga
      661 tttcgcgaaa attgcttcca tcgcgctcgg gccggcattg tagacgtacc actcgatctt
      721 gacgtccggc ccaagtcgct gctcgaacca gccctttccc tggcgtgaca tgttatgcgc
      781 gaccaacgcc tgaacatgcg tgatgttggg aaaatgaccc atccgcactg tcgtttgcgc
      841 cggcgattga accgtcgata tcagcaagga catcacgcac gacgtcagga acgctgcact
      901 ggtccacgat tgacgcagct tgaccaggtc gcacatcgct cttgccccca ttcgttgttt
      961 cggaatctgg cagaaagcgt ttcgaaaaaa cctattcaca caacgacgac ctttaacaag
     1021 atcacaaatc actctttgct tcgctgccca aagaagtttc ctgccaaaga aactacacga
     1081 atagaaattg gttctctccg atctgactat ttgcaaagcc aggtttatta tagaaaaaaa
     1141 tagcgactga gtgctcgttt ttggaatgaa tactcgtatc gccatttaaa tttggaattc
     1201 caaaggatcg ggccgcgcca tatctactgg agaaggtgca caacctgccc acgctgcgct
     1261 ttgcggaaga accgatgcga ctcaaactaa gaagcaatgt ttttgtctct gtcacatccg
     1321 gtcgttttct gcgttcacgg agtgacattg atggaccgcc tggattaact cgaagcacag
     1381 agtatcttcg tcttgcgcga ggcgtttgcc cgcctgaagc gcatcgccct gctttggtcg
     1441 cttggaaaag attccaatgt cctgatctgg cttgcgcgca aggccttctt cggccacttg
     1501 ccgtttccgg tcatgcatgt ggatacgggc aagaagttct ccgagatgta tgccttccgc
     1561 gaccgatatg cgcgggagtg gcagctcaat cttctgatcg aacactgtcc tccggtggaa
     1621 accatcgacc caacactacc gccggcagcg cgatctgcgg cacgcaagac tgaaggactc
     1681 aagctcgccc tggccaagca cgggttcgat ggattgatcg ccggaatccg gcgcgacgaa
     1741 gagccgacgc gcgccaaaga gcgcgtgttt tcgccgcgtg gctccgagaa cctgtgggat
     1801 gtgcgcgacc agccgcccga gttctggaat catttcaaca ccgcgccgct atctggcgct
     1861 cacctgcgcg tgcatccgat cctgcagtgg accgaagccg acatctgggc ctacacccgg
     1921 cgtgaatcta ttccaatcat tccgctctat ctggcaagaa acgggaagcg ttatcgttcg
     1981 ctcggcgacg ccgacatcac ctttccagtg acctccaccg cgcgaaccat cgatcaaatc
     2041 atcgccgaag tgaacacaac caatattccg gagcgatcgg gccgcgcgat ggaccatgag
     2101 accgaggacg ccttcgagcg gctgcgaacc gcaggttatc tgtgacgaat tgaatgtgac
     2161 gccatgaatg tcgctg
//
LOCUS       DASTPM010000339         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_502050, whole genome shotgun sequence.
ACCESSION   DASTPM010000339 DASTPM010000000
VERSION     DASTPM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502050"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VFR42_05950"
     CDS             <1..537
                     /locus_tag="VFR42_05950"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEU0368729.1"
                     /translation="NARAKDKEEELHAIADLLQWANEAGLLSPADHERLAAHYSRSSR
                     EAAAALTKAITIRDLVLSIFMSIANGRSLPSQRLSELNSALAEVPGLLRVQKHSDRIE
                     REWTSTADGLQQVLFAVLTSAAELLASDRLRRVRECASADCTWLFVDESRNRSRRWCE
                     MRACGNRMKARRHYYKDR"
     gene            1284..>2035
                     /locus_tag="VFR42_05955"
     CDS             1284..>2035
                     /locus_tag="VFR42_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368730.1"
                     /translation="MAKRLAVFVYGLVSYVAFFLTFVYAVGFIGNLYVPKSMDSGARM
                     SFFPALVIDALLLLMFAVQHSVMARPAFKEVLTRFIPAAAERSTYVLCSSVLLIALFA
                     FWQPLGGGVWNVTNPVLRNAINAVFGFGFALVFVATLLINHFDLFGLRQVALYLAGKP
                     YTYLEFRTPLFYRFVRHPLYVGWLIAFWATPTMTGAHLLFAVLTTTYILMAIRWEEHD
                     LVVVHGSKYQDYQKRVPKLIPSLAPYREADRQ"
BASE COUNT          393 a          592 c          572 g          478 t
ORIGIN      
        1 aatgcgcgcg ccaaggacaa agaggaggaa ctacatgcca tcgcggatct gttgcagtgg
       61 gcgaatgaag cagggctgct ttcgccagca gaccacgagc gtctagccgc acattacagc
      121 cgcagttccc gtgaagccgc agctgcgtta acgaaggcca tcaccatccg cgacctcgtg
      181 ctctcaatct tcatgagcat cgcaaatggt cgttcgctgc catcccagcg tctgtcggag
      241 ttgaattcgg ccctggcaga agttccagga ctgctgcgtg tgcagaagca ctccgaccgg
      301 attgaaaggg aatggacgag taccgccgac gggttgcagc aggtcctttt cgcagtcctc
      361 accagcgcag cagagctgct cgccagcgat cgcctgcggc gagttcgcga gtgcgcctcc
      421 gccgattgca cctggttgtt cgtcgacgag agccgcaacc gcagtcgacg ctggtgcgag
      481 atgagggcgt gtggaaaccg catgaaagcg cggcgccatt actacaagga tcgctagcac
      541 agccttggtg gcccaacaat acgcaacctg gaacttaggc ctttatgccg gaccatcagg
      601 tgaaaaacta ggccctcacc actactttgc tcccgcacgg cgagtaagcc agagcttttg
      661 ggttgaagac taggcaagga ccgagttcta tctctggctt cgagctgctc taagcgtcgg
      721 aggcttacca ccactgctta ccgcgcgatg gctgccgtga tcatgcctct ctcggcatcc
      781 aggggaaaga gaaggccatt ttaattccgg tctcatgcag agatcccggt tcgcggtttc
      841 gtcacttggg atgcagcgcc gactgcgtta gcgagtcggc ggccagggta agtcaatggc
      901 tttcatgatt tcctcggccc accgcttcgg cgccagaacc ttcttgccgc catcgaactg
      961 agtcaatttg cgtggcaagg atactagctg cactcaacag cacgcgtttg cggccctcgt
     1021 ccacgcagac gtgtctagca gtctcgcgtt ttgttcgcca aggcaggcgt aattcgagcc
     1081 cgtgattcta gcccgtgatt tcatttcgca atcccttttt cttcattccg ctggaattca
     1141 atcgcgccgc tgtttctcag aatcttctca gcgagatttc aggatcccca aaggacgttc
     1201 acaccagtga gccgctagtg ttgcacccat ctcacggaga acctggagca atggccaggt
     1261 cccaacaaaa gaggatggtg ggtatggcaa agcgtttagc agtatttgtt tacggactgg
     1321 tcagttacgt cgcgttcttc ctgacattcg tgtatgccgt cgggttcatc ggcaatctgt
     1381 atgttccgaa gtctatggac tccggggccc gaatgtcgtt cttcccggcc ctcgtcattg
     1441 atgccctgct gctgctgatg ttcgcagtac agcacagtgt catggcgcgg cctgcattca
     1501 aagaagtgct gactcgattt attccagcgg ctgccgaacg cagcacctat gtactgtgct
     1561 ccagcgtgtt gctgatcgcg ctgtttgcat tttggcagcc cctcggcggc ggcgtttgga
     1621 acgttaccaa tcctgtcctt cggaacgcca tcaatgccgt attcggcttc ggtttcgctc
     1681 tggtgtttgt tgccacgctc ttgatcaacc actttgacct gttcggcctg cggcaggtgg
     1741 cgttgtatct tgccggaaag ccgtacacgt acctcgaatt ccgcacgccg ctgttctacc
     1801 gctttgtgcg acatccgttg tacgtgggtt ggttgatcgc gttttgggct acgcccacga
     1861 tgacgggcgc gcacctgctg tttgcggtgc ttacgaccac ctacatcctg atggcgatcc
     1921 gctgggaaga gcacgatctc gtcgtcgttc acgggtcgaa gtatcaggat tatcagaagc
     1981 gcgtgccgaa gctgattccg tcgttggctc cgtaccgcga agcggaccgg cagca
//
LOCUS       DASTPM010000340         6319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_646077, whole genome shotgun sequence.
ACCESSION   DASTPM010000340 DASTPM010000000
VERSION     DASTPM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646077"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VFR42_05960"
     CDS             <1..855
                     /locus_tag="VFR42_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368731.1"
                     /translation="TIGLGGTEWRWKLGIAALPAAIFLLMLLGIPRSPRWLAKKGRVE
                     EAREVLRITGEENYEQELKDIVASIDAEHHAEDTLFNGKYGLPIFLAVSIAMFNQLSG
                     INAILYYLNQIFAQAGFSKVSSDLQAVAIGATNLIFTMIAMTMIDRVGRKKLLLTGAV
                     GTTLCLAGVSYVFLSHSHENWLVWLLVAYIAFFAFSQGAVIWVYIGEVFPNRVRAAGQ
                     SLGSFTHWVMAGIISLGFPAVAAKSGGYPFVFFSVMMALQFFVVLLWYPETKGITLEE
                     MQKKLGIA"
     gene            complement(903..3518)
                     /locus_tag="VFR42_05965"
     CDS             complement(903..3518)
                     /locus_tag="VFR42_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368732.1"
                     /translation="MQISLRLSLTALLLATSAETFLVPAHSPDPNAAASTPTLEISRP
                     ARPWEFLAAVGKKASLLGNESGAVEAWVYPLKILRDLRLTILTEGRAIPAESLVRTVI
                     AHPESTTLLYSSDTFSIRETFFAPVDQPGAVIELQVESEQPLELEASFIRDFQLEWPA
                     SMGGTYVGWSDPLHAFFFGEDRQKFSALAGSPSATSPQLEYETNYGGTHRSSMRLGIT
                     SQGRDTKLIVIAGSINGRAEAETAYKQLTADYEKLRRESAAFYQQYLQRTVSVALPDA
                     DLERAYDWSRISLIQGLVSNPTMGDGLIAGYRASGESYRPGFAWFFGRDSLWCSLALN
                     SEGDFATTKTALEFIAKFQREDGKIPHEIAQGASFVDWFKAYPYAYASADATPLFIVG
                     IDDYVTSSGDIEFAKANWPHIAKAYEFLRSTYNANGFPLNFGIGHGWVEGGPLLPIES
                     EFYQSGLATAAVRSLAHLSQLTGKDADVPALSKQFEKERTHLNQAFWQADKKTFAFAQ
                     GRDNQRVDEPSVLATVPMWFGLTDEDKSQQMISALAAHEHQTDWGMRIISNRAEKFSG
                     GGYHYGSVWPLFTGWASVAEYRYHQPQPAYDNLRANALLALDGSLGHVTEVLSGDYYQ
                     PLSTSSPHQIWSAAMVISPLLRGLFGLQADATNHTLMLSPHLPAGWSTFSLENVRVGE
                     NRLLINYKRVEQGILLEVGRTEGKDDCMVEFRPAVSPRARIQKADLNGNPIPFHLEQH
                     AIDQHVVVRFPLRDHKTFLRLFLTNDFSIGDDAALPALGGVSEGLRIISETWSSSKDR
                     LTLDVSGKASKTYLLKVADDYNQIERIEGAVGTGLNHSQLTGSDFITIDMPQDADRYP
                     RKTIVINFKTKSPVK"
     gene            3820..5520
                     /locus_tag="VFR42_05970"
     CDS             3820..5520
                     /locus_tag="VFR42_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU0368733.1"
                     /translation="MQKLEGGNSAALGVSFLDVAKQAGLNAKTIYGGEHKNKYLLETT
                     GCGVAFYDYDNDGWLDIFLVNGWRLEGFPKGQEPHSHLFKNNRDGTFTDVTEKAGLVH
                     TGWGQGVCIGDYDNDGYEDLFVSYFGKNVLYHNNGNGTFADVTERAGVGGNGKRWNSG
                     CAFVDYDRDGKLDLFVANYIDMDLATAPVPESGPCLYKGVMVACGPPGLIGGKNILFR
                     NNGDGTFTDVSETSGILSANGTYGLGVLTGDFDNDGWPDIYVANDSTASALYQNKKNG
                     RFTDIAMEAGCALSADGKPQAGMGVSAADYDMDGNLDIVKTNFAGDTPSLYHNVGNAT
                     FEDTTFTGGLGKHTQYLGWGCGFFDFDNDGWPDILICNGHVYPEVEQLTTEAGYAQRK
                     LLYKNLRNGKFDDVSLHAGAGISTPSASRGCAFGDFDNDGDLDVVVNCVNDFPQLLRC
                     DSTLKNNWIKVRTIGTKSNRSGIGARLRCVTRGAGEEAPHSQIDEVRSGGGYYSQNDL
                     RVHFGIGTAEKVELLEIRWPSGQVDALKDLKANRLYYIREADGVTRTVVFPPSKKESA
                     "
     gene            5705..>6319
                     /locus_tag="VFR42_05975"
     CDS             5705..>6319
                     /locus_tag="VFR42_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0368734.1"
                     /translation="MVDRRRFLATFSGMGFGSTLLPGVLWALADGKAEITEAMIEQAA
                     AIADVPIPAEYRKTMLESLNDHAKNFQEIYKLRIPNSVAPALVFDPVLPSTKFETQKR
                     PMKISSAPSIAARGVPENLEDVCFASARELAELVRTKKVSSMALTQMYLQRLKRYHPL
                     LHFVITLTEERALTKAREADRDLAAGKYRGPLHGLPWGAKDLLAV"
BASE COUNT         1360 a         1438 c         2153 g         1368 t
ORIGIN      
        1 acgattgggc tgggcgggac ggagtggcgg tggaaactgg ggattgcggc gctacctgca
       61 gcgatttttt tgttgatgct attggggatt ccgcggagtc cgcgctggct agcgaaaaaa
      121 ggccgcgtgg aggaagcgcg cgaggttctg cgcattaccg gcgaggagaa ttacgagcag
      181 gagctaaagg acatcgtggc ctcaattgat gcggagcatc acgcggagga cacgctgttc
      241 aacggcaagt atgggttgcc gatttttctg gcggtgtcga tcgcgatgtt caaccagctt
      301 tcggggatca atgcgatttt gtactacttg aaccagattt ttgcgcaggc gggattcagc
      361 aaagtgtctt cagatttgca ggcggtggcg attggagcga ccaatctgat ttttacgatg
      421 attgcgatga cgatgattga tcgcgtgggg cggaaaaaac tgctgctgac gggcgcggtg
      481 ggaacgacct tgtgcctggc gggcgtgtcc tatgtgtttt tgtcgcacag ccatgaaaac
      541 tggttggtgt ggctgctggt ggcgtacatc gctttttttg cattttcgca aggcgcggtg
      601 atctgggtgt atatcggcga agtgtttccc aaccgggtgc gggcggcggg gcagagcctt
      661 gggagcttta cccactgggt gatggccgga atcatttcgc tgggatttcc ggcggtggcg
      721 gcgaagtctg gcgggtatcc gtttgtgttt ttctcggtga tgatggcgct gcagtttttt
      781 gtggtgctgc tgtggtatcc ggagacgaag gggattacgc tcgaggagat gcagaagaag
      841 ctggggattg cttgaggaat agagatcgcg cagagacgcg gaggcgcggg caaaaataga
      901 atctatttca cgggagattt tgttttgaaa ttgatgacga tggtcttgcg cggatatcgg
      961 tcggcgtctt gcggcatgtc aattgtgatg aaatcgctgc ccgtcagttg cgagtggttc
     1021 aggccagttc ctaccgcacc ctcgatcctt tcgatttgat tatagtcgtc cgcgaccttg
     1081 agaaggtatg tcttgctggc ttttcctgaa acatctagcg tgagccgatc cttggaggac
     1141 gaccaagtct cggaaatgat cctcaatcct tcacttacac ctccaagtgc tggaagtgcg
     1201 gcgtcatcgc ctatgctgaa atcgttggtc agaaaaagtc ttagaaaagt cttgtggtcg
     1261 cgaagtggaa aacgcaccac gacatgctga tctattgcgt gttgttcgag gtgaaacgga
     1321 atgggattgc cgtttaagtc ggccttttgg attcgggcgc ggggactgac ggcaggacga
     1381 aactcaacca tgcaatcgtc tttgccctca gttcgaccaa cttcgagaag gatcccctgt
     1441 tccactcgct tatagttgat gaggagccga ttctcgccaa cgcgaacgtt ttcgagggaa
     1501 aacgtcgacc agccggctgg caagtgcggt gacagcatca aagtgtgatt agtagcgtcc
     1561 gcttgcaagc cgaaaaggcc gcgcaggagc gggctgatga ccatggcggc agaccagatt
     1621 tgatgaggag agctggtgga aagcggctgg tagtaatcgc cggagagaac ttcagtaacg
     1681 tggccgagtg agccgtcgag cgcgagcaga gcgttggctc gcaggttgtc gtacgcgggc
     1741 tgcggctggt ggtagcgata ttcggcgacg gaagcccaac cggtgaagag cggccagacg
     1801 gaaccgtaat ggtagccgcc gccgctgaat ttttcggcgc gattggaaat aatgcgcatg
     1861 ccccagtcgg tctgatgttc gtgggcggcg agcgcggaaa tcatctgctg cgatttgtct
     1921 tcgtcggtga ggccgaacca catgggaacg gtggcgagga cgctgggctc gtcgacgcgc
     1981 tggttgtcgc ggccttgcgc gaaagcgaaa gtttttttgt cagcttgcca aaaggcttgg
     2041 ttcaagtgtg tgcgctcttt ttcgaattgc ttggagagag ccggtacgtc ggcgtccttg
     2101 ccggtgagct gcgagagatg tgcaagcgag cggacggcgg cagtggcgag accactctgg
     2161 taaaactcgg attcgatggg aagaagcggg ccgccttcga cccagccgtg gccgataccg
     2221 aaattgagcg ggaagccatt ggcgttgtag gtggagcgca ggaattcgta agccttggcg
     2281 atgtgcggcc agttggcttt ggcgaattcg atgtcgccgc tggaagtgac gtagtcgtcg
     2341 atgccgacga tgaagagcgg agtggcgtcg gcggaagcgt aggcgtaagg ataagccttg
     2401 aaccagtcga cgaagctggc gccttgcgca atttcgtgag ggatttttcc gtcttcgcgc
     2461 tggaatttgg cgatgaattc gagcgcggtt ttggtggtgg cgaaatcgcc ttcgctgttc
     2521 agggcgaggc tgcaccagag agagtcgcgt ccgaaaaacc aggcgaagcc ggggcggtaa
     2581 ctttctccgg aagcgcgata accggcgatg aggccgtcgc ccatggtggg attggaaacg
     2641 agtccctgga tgaggctgat gcgggaccag tcgtaagcgc gctctagatc ggcgtcggga
     2701 agcgcgacgc tgacggtgcg ttgcagatat tgctgataga aagcggcgga ttcgcggcga
     2761 agcttttcgt agtcggcggt gagttgtttg taggcggttt cggcttcggc gcggccgttg
     2821 atggaaccgg cgatgacgat gagcttggtg tcgcggccct ggctggtgat gccgaggcgc
     2881 atggagctgc ggtgcgtacc gccgtagttg gtttcgtatt cgagttgtgg gcttgtggcg
     2941 ctgggcgagc cggcgagcgc ggagaatttc tggcgatctt cgccaaagaa aaaggcgtgc
     3001 aatggatcgc tccagccgac gtaggtgccg cccatggagg cgggccactc aagttggaag
     3061 tcccggatga aactggcttc gagctcgaga ggctgctcgc tttcgacttg gagttcgatg
     3121 acggcgccgg gctgatcgac gggggcgaag aaggtttcac ggatcgagaa agtatcactg
     3181 gaatagagga gggtggtgga ttcgggatgc gcgatgacag tgcggacaag agattctgca
     3241 ggtatggcgc gaccttcggt gaggatggtg aggcggagat cgcggagaat tttgagagga
     3301 tagacccaag cttcgacggc gccggattcg ttgccgagga ggctggcttt tttgccgacg
     3361 gcagcgagaa attcccacgg acgagcgggg cgggagattt cgagcgtcgg agtagaggcg
     3421 gcggcgttgg ggtctggcga atgcgccgga accaaaaatg tttcggccga agttgcgaga
     3481 agcagagctg ttaacgacag gcgcagagag atttgcatgg cacaattatg ggtagcgcgt
     3541 gttccttgca atgcgttgcc gcactgccta ttttagtcag ggtttgtaaa agacttattt
     3601 acccctggtt tgtaagacaa gccctgaaac ccgaaacggg cagagtgtag cacgcgtttt
     3661 tgttcgacca aagggaaggt aactcgtcga ttgtttttcc cacgcagaag gttccttaaa
     3721 tcgctgagcc ggtcagcact tgtcttgtcg ctggagaacc tgcttgcagc agttcgaccg
     3781 gcgtttggcg gtgcaaagcc cagtgagcgg tggacaccgg tgcagaagtt ggaaggaggg
     3841 aattccgcgg cgctgggcgt gagttttctg gacgtggcga aacaagcagg gctgaacgcc
     3901 aagacgatct atggcggtga acacaagaac aagtatctgc tggagacgac cggttgtggc
     3961 gtggcgtttt atgactacga caacgacggc tggctggaca tttttctggt gaacggctgg
     4021 cggctggaag gatttccgaa agggcaggag ccgcactcac atctattcaa aaacaatcgc
     4081 gacggaactt ttacggatgt gacagaaaaa gccgggctgg tacatacggg ctggggccag
     4141 ggcgtgtgca tcggcgacta cgacaacgac ggttacgaag atttgtttgt gagctatttc
     4201 ggaaaaaatg ttttgtatca caacaatggc aacgggacgt ttgccgacgt gacggaaaga
     4261 gcaggggtcg gtggaaacgg aaagcgctgg aattcgggct gcgcgtttgt ggactacgac
     4321 cgcgacggca agctggacct ctttgtcgcc aactacatcg atatggatct ggccacggcg
     4381 ccggtgccgg aatcggggcc atgcctgtac aaaggagtga tggtggcgtg cgggccgcca
     4441 ggtctgatcg gcggaaaaaa tattttgttt cgcaacaacg gcgacggaac tttcacagat
     4501 gtgtcggaga cgagcgggat tctcagtgcg aacggaacgt atgggctggg tgtgttaact
     4561 ggggattttg acaacgacgg ctggccggac atttatgtgg cgaacgattc gacggcgagc
     4621 gcgttgtatc agaacaagaa aaacggaagg tttacggaca ttgcgatgga ggcgggctgc
     4681 gcgctgagcg cggacgggaa gccgcaagcg ggaatgggag tttcggcggc ggactacgac
     4741 atggacggca acctggacat tgtgaaaacg aattttgcgg gagatacccc gtcgctttat
     4801 cacaatgtcg ggaatgcgac gtttgaagat acgacgttca cgggagggct ggggaagcac
     4861 acacagtatt taggatgggg ctgcggattt tttgattttg acaacgacgg ctggccggac
     4921 attttgattt gcaatggcca cgtgtacccg gaagttgagc aattgacgac ggaggcgggc
     4981 tacgcgcagc gaaagttgct ttacaagaat ttgcggaacg gaaaattcga cgacgtttcg
     5041 ctgcatgcgg gggcggggat ttctacacca tcggcttcgc gaggctgtgc gtttggggat
     5101 tttgacaacg acggggatct ggatgtggtg gtgaattgcg tgaacgattt tccgcagctg
     5161 ctgcgctgcg attcgacgct gaagaataac tggatcaagg tgcgcacgat cgggacgaaa
     5221 tcgaatcgca gcggaatcgg ggcgcggctg cgctgtgtga cacggggggc gggcgaagag
     5281 gcgccgcatt cgcaaattga tgaagtgcgc agcggcggcg ggtattattc gcagaacgat
     5341 ctgcgcgtgc acttcggaat cggcactgcg gaaaaggtag agctgctaga gatccgctgg
     5401 ccgagcgggc aagtggatgc gttgaaggat ttgaaggcga atcgtctgta ctacataagg
     5461 gaggcggatg gggtcacgcg gaccgtggtg tttccgccgt ccaagaagga atcagcctaa
     5521 agcgcccgga aaaccaactc ctcaagggct aaagcccgaa tcatagccaa gcgctgatgt
     5581 cggaacgaag ctccgacccc tgcagcaact tccctctgaa gggagcttca agtacaagca
     5641 ctttggagtc tgcccttcgc gtaatagaat cccgcagcat tcattttcct tgggagatta
     5701 cgcgatggtg gatcgacggc ggtttcttgc gactttttcg gggatggggt tcggttcgac
     5761 gctgttgccg ggtgttctgt gggcgctggc agacggcaaa gcggaaatta ccgaggcgat
     5821 gatcgaacag gcagcggcca tcgcggacgt gccaatcccg gcggaatacc gcaagacaat
     5881 gctggagagc ctgaacgatc acgccaaaaa cttccaagaa atctacaaat tgcgcattcc
     5941 gaacagcgtg gcgccggcgc tggtgttcga tccggttttg ccgtcgacga aattcgaaac
     6001 gcaaaagcgg ccaatgaaga tttcgtcggc gccgtcgatc gcggcacgtg gagtaccgga
     6061 gaaccttgag gacgtgtgct ttgcttcggc gcgcgaactg gccgagttgg ttcgaaccaa
     6121 gaaagtttca tcaatggcgc tgacgcaaat gtatctgcag cggttgaaac gctaccaccc
     6181 gctgttgcac tttgtgatta cgctaaccga agagcgcgca ctgacgaagg ccagggaagc
     6241 ggaccgggac ctcgcagccg gaaaatatcg cgggccgctg cacgggttgc cctggggcgc
     6301 caaagatctg ctggcggtg
//
LOCUS       DASTPM010000341         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1222841, whole genome shotgun sequence.
ACCESSION   DASTPM010000341 DASTPM010000000
VERSION     DASTPM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222841"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFR42_05980"
     CDS             <1..1293
                     /locus_tag="VFR42_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEU0368735.1"
                     /translation="GLRRLVAVVCASALLLVCPEQGKAYSVMSHQAIIDAAWEGGLKP
                     ALKQRFPSMTEEDFKKAHAFAYGGAIIQDLGYYPYGNPFFSDLTHYVRSADFILALLR
                     DAQDPNEYAFALGAMAHYASDNNGHKIGTNRAVPILYPKLKKKYGDSVSYEDDKLAHV
                     KTEFGFDVLQVAKERYAPESYHDFIGFEVSRRVLEEAFEETYGLDLKKVLVNEDRALN
                     SYRHAVSDLLPRATRIAWHLKQDQIEKDQPGVTKRKFLYNLKRAKYEQEWGTDYQRPT
                     FGDKCLAFLYRILPKFGPLRVLEFRTPTPETEKMFEASFNGTLDHYRELIRQQRETQR
                     PAMTNDNFDLGTTSAPGEYQLSDSTHAKLLDKLAEQNFAGLTPEIRTELLEFFGAPDT
                     PFQLKKDKKAWTKLQTDLEALRKLPTKETVASASATEN"
     gene            1437..>3451
                     /locus_tag="VFR42_05985"
     CDS             1437..>3451
                     /locus_tag="VFR42_05985"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF009554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEU0368736.1"
                     /translation="MSKPKPIQKLLAANRSEIAIRIFRAANELGLRTVAVYSKEDRLA
                     LHRFKADEAYLVGEGKGPVEAYLDIAGIVAVAKAKGVDAIHPGYGFLSENPALARACE
                     KAGITFVGPTPALLELLGDKTAARGLAIAAGVPVLPGTKEPVKSAEQAKKIAAEIGYP
                     VMVKAAMGGGGRGMRVVRDESELEARLEEAQSEAKSAFGDTSVFLEKYLARARHLEVQ
                     ILADTHGNLLHLWERDCSVQRRHQKVVEVAPAPNLPASVRNELCAAAAQLARKAKYRN
                     AGTVEFLYDVDAQKWYFIEVNPRIQVEHTVTEMVTGIDIVRAQILVAQGHGLHDEAMN
                     LPKQESVPLYGAALQCRVTTEDPEKNFAPDYGKLTTYRSPAGFGIRLDGGTAYAGASL
                     APYYDSLLVKVTAWGTNLPEACQRMDRALREFRIRGVKTNIPFVENVVNHAKFRTGHV
                     TTGFLEQHPELFRFPKRGDRATKLLTYLGDVILNGNAEVKGKPVPSALEVAAVPAAST
                     TAAAKAGTRQLLKKLGPAKFAEWAKREKRLLLTDTTFRDAHQSLMATRVRTYDLLATA
                     PFVAERLANLFSLEMWGGATFDTALRFLREDPWQRLRQLRAAVPNICFQMLLRGANAV
                     GYTSYPDNVIREFVRVAYAEGMDIFRVFDSLNSIENMRVSLDAVLETG"
BASE COUNT          797 a          808 c         1183 g          663 t
ORIGIN      
        1 gggttgcgac ggctcgttgc ggtggtttgt gcgagcgcac tgctgctggt ttgcccggag
       61 caggggaagg cctactcggt gatgtcgcac caggcgatta ttgatgcggc atgggaaggc
      121 ggcttgaaac ccgcactgaa acaaagattc ccgagcatga ccgaagaaga tttcaaaaaa
      181 gcacatgcgt tcgcttacgg cggagcaatt attcaggatc taggctacta cccttatgga
      241 aatccgtttt tcagtgactt gacgcattat gtgcgaagcg ccgattttat tttggcgctg
      301 ctaagagatg cgcaagaccc taatgaatat gcctttgcat tgggagcgat ggcgcactac
      361 gcatcagata acaatggcca caagatcgga acgaatcggg cggtgccgat tctttacccg
      421 aagctgaaga aaaagtatgg ggattcagtt tcctacgaag acgacaaact ggcgcacgtg
      481 aagacggaat tcggatttga cgtgctgcaa gtggcgaagg agcgttatgc gccggagagt
      541 tatcacgact tcatcggatt tgaagtttcg cgcagggtgt tggaagaagc gttcgaagaa
      601 acgtacggac tggatttgaa aaaggtgctg gtgaatgagg accgtgccct gaattcctac
      661 cggcacgcgg tcagcgacct gctgcccagg gccactcgga tcgcctggca cctgaagcag
      721 gatcaaatcg agaaggatca acccggcgtt acgaagagga agtttctcta caatctgaaa
      781 cgtgcaaagt atgagcaaga gtggggcacg gactaccaac ggccgacgtt tggcgacaag
      841 tgcctggcgt tcctgtaccg gattttgccg aagttcggac cgctcagggt tttggagttc
      901 aggacgccga cgccggaaac ggaaaagatg tttgaagcca gttttaatgg cacgctggat
      961 cactacagag agctgatcag gcagcagcgc gagacgcagc ggccggcaat gaccaacgac
     1021 aattttgatc tgggcactac cagcgcgccg ggcgaatacc agttgagcga cagcacgcat
     1081 gcgaagctgc tggacaagct ggcggaacag aatttcgcgg gattgactcc agagattcgg
     1141 actgagttgc tggaattctt cggcgctccg gataccccat tccagttgaa gaaagacaaa
     1201 aaagcctgga ccaagctgca aacagacctg gaagcgctgc ggaaattgcc tactaaagaa
     1261 acggtcgcaa gcgcctcggc gaccgagaat tagggaagaa aggcgcagat tcagtaggac
     1321 gccaacggat tacttcgcat tgaaaacaca actttgattg attaaaaaaa ctgctgcggt
     1381 gctatggggt ggccgtgaaa gaatgaacgg ttgcgcaccc ttgcggtagc gaaactatgt
     1441 ccaagccaaa gcccatccaa aagctgttag cggcgaaccg aagcgagatt gctatccgga
     1501 tctttcgggc ggcgaacgaa cttggcttgc ggaccgtagc cgtgtattcg aaagaggacc
     1561 ggctggcgct gcaccgcttc aaggcggacg aagcgtacct ggtgggcgaa gggaaggggc
     1621 cggtcgaggc gtacctggac atcgcgggaa ttgtggcggt cgcgaaagcc aaaggcgtgg
     1681 acgcgattca tccgggttat ggattcttgt cggagaatcc ggcgctggcg cgggcttgcg
     1741 aaaaggctgg aataactttt gtggggccga caccggcgtt gctggaattg ctgggagaca
     1801 aaaccgcggc gcgagggctg gcgattgcag cgggcgtgcc ggtgctgccg ggtaccaagg
     1861 agccagtgaa gtcggcggag caagcaaaaa agattgcggc ggaaattggt tatccggtga
     1921 tggtgaaagc cgccatgggc ggcggtgggc gcgggatgcg cgtggtgcgc gacgaaagcg
     1981 agctggaagc gcggctggaa gaggcgcaga gcgaggcgaa gtcggcgttt ggggatacgt
     2041 cggtattctt ggagaaatat ttagctcgag cgcggcacct ggaagtgcag atacttgccg
     2101 acactcatgg aaatttgctg catttgtggg agcgggattg ttcggtgcag cggcggcacc
     2161 agaaagtggt ggaagtggcg ccggcgccga acttgccggc gagcgtgcgg aatgaattgt
     2221 gcgcagcggc ggcgcaactg gcgcgcaagg cgaaataccg caacgcgggt acggtggagt
     2281 ttttgtacga cgtggatgcg cagaagtggt acttcatcga ggtgaatccg cggatccagg
     2341 tggagcacac ggtcacggag atggtgactg ggattgatat tgtgcgggcg cagattctag
     2401 tggcgcaggg gcatgggctg catgacgagg cgatgaatct gccgaaacag gagagcgtgc
     2461 cgctgtacgg ggcggcgctg caatgccgcg tgacgacgga agatccggaa aaaaatttcg
     2521 ctccggatta tgggaagctg acgacgtacc gatcgccggc gggatttggg attcggctgg
     2581 atggcgggac ggcgtacgcg ggagcttcgc tcgcgccata ttacgactcg ctgctagtga
     2641 aggtgacggc gtggggcacg aatttgccgg aagcctgcca gcgaatggac cgcgcgctac
     2701 gcgagtttcg catacgcggc gtcaagacga atatcccgtt cgttgaaaac gtcgtgaatc
     2761 atgcgaaatt ccgcaccggc cacgttacga cggggtttct ggagcagcat ccggagttat
     2821 tccggttccc gaagcgcggc gatcgcgcga cgaagctgct gacgtatctg ggcgacgtga
     2881 ttttgaacgg gaatgcggaa gtaaaaggga agccggtacc gtcggcgttg gaagtggcag
     2941 cggtacctgc ggcgagcacg actgctgcgg ccaaggcggg tacgcggcag ctgctgaaga
     3001 agctggggcc ggcaaaattt gcagagtggg caaaaagaga gaagcggttg ctgctgacgg
     3061 atacgacatt tcgcgacgcg caccaatcgc tgatggcgac gcgggtgcgg acgtacgact
     3121 tgttggcgac tgcgccgttt gtggcggagc ggctagcgaa cctattcagt ctggagatgt
     3181 ggggcggggc gacgttcgat acagcgctac ggttcttgcg ggaggatccg tggcagcggc
     3241 tgcggcagct gcgcgcggcg gtgccgaata tctgttttca aatgctgctg cgcggtgcga
     3301 acgccgtagg ctacacgtcg tatccggata acgtgatccg cgagtttgtg cgcgtggcct
     3361 acgcggaggg gatggacatt tttcgcgtgt tcgattcgct gaattcaatc gagaacatga
     3421 gggtgtcgct ggatgcggtg ctggaaacgg g
//
LOCUS       DASTPM010000342         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_386908, whole genome shotgun sequence.
ACCESSION   DASTPM010000342 DASTPM010000000
VERSION     DASTPM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386908"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFR42_05990"
     CDS             <1..982
                     /locus_tag="VFR42_05990"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0368737.1"
                     /translation="MRRREFILVLGTATALPLASLAQQEKKVFRIGYLSAPTRESVQR
                     PLEVFLRKLRELGWVEGENFVIEYRWAEGNIIRLPELASELVKLNVDLIVAPATSAAL
                     AAKNATSRIPIVMTFPGDPVELGLVSSLSQPGGNVTGTTAAAGPGIIGKLLEILKQAV
                     PHITAVAILGNSADPGLASQMKELRVAAESLSLRLQLFESRGREEFDHAFAEMTRARM
                     DGLAIMGATFVPHRAKLAELAMTARLPVITFLREFTEAGALLSYGVNMSDFIGRAAVY
                     VDRILKGARPADLAVEQPTKFELAINLRTAKALGLSVPPVLLATADEVIE"
     gene            complement(1086..1721)
                     /locus_tag="VFR42_05995"
     CDS             complement(1086..1721)
                     /locus_tag="VFR42_05995"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HEU0368738.1"
                     /translation="MANTREPILQPIPILSLHPTQMTVGMREVKEKRKRLREHWRKHK
                     KIKQGQFLGRHMIPVVLGPDQRHYVVDHHHLARALHDEGVEDIIVTVIADLTMVDRDA
                     FWGVLDNHRWAYPYDAKGQRQHFRNLPKTVVELKDDPFRSLAGELRRVGGFAKDTTPF
                     SEFLWADFLRRRLSRKSVEANFDKAIEKALALAKSRDAVYLPGWCGPTSDD"
     gene            1831..>2629
                     /locus_tag="VFR42_06000"
     CDS             1831..>2629
                     /locus_tag="VFR42_06000"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU0368739.1"
                     /translation="MIKGRSERTANKRVVLLGGREVIAEGVRLNFWADISHRCMTASW
                     PSFIAGAALVFVVFNAVFAVFYWIGNQPISNVPDGAYIDYLYFSIETLSTAGYGDMHP
                     QTHYGHFIATVELFTGIFSMSLMTGLIFARFSRPNARLLFADHPVISSHEGKPTLMIR
                     FANERHDIIGNATARLWLLKNVVTTEGQSLRRFYELSLVQNEHPALALSWTLYHILDE
                     QSPLYGLNADDLAASDVSLVVVVSGYDVVAAQIVHARKSYDYRHIRFG"
BASE COUNT          505 a          774 c          772 g          578 t
ORIGIN      
        1 aatgaggcgg cgggagttca tcctggtgtt gggaacagcg acggccttgc cgttagctag
       61 ccttgcgcag caggagaaga aggtctttcg catcggctat ctaagtgccc ccacccggga
      121 atcggtccag cgtccgcttg aggtgttctt gcgaaagctg cgcgagctgg gttgggttga
      181 aggcgagaac ttcgtcattg aatatcgatg ggctgaggga aacatcatca ggttgccaga
      241 gcttgcaagc gaactggtga agctcaacgt agacctgatc gttgcaccgg ccacctcagc
      301 ggccctggcg gcaaagaacg cgaccagccg tatcccgatc gtcatgactt tcccgggcga
      361 tcctgtggag ctgggactcg tttccagcct tagtcagcct ggtggaaacg tcaccgggac
      421 gactgccgcg gcaggcccgg gaattatcgg caagcttctg gaaatcctga agcaggccgt
      481 tcctcacatc accgcggtcg cgatcctggg caattcagcg gacccgggtc ttgcttcaca
      541 aatgaaggag cttcgcgttg ccgcggagtc gctgagcctt cgccttcagc tcttcgaatc
      601 gcgaggtcgg gaagagttcg atcatgcatt tgccgaaatg acgcgggcac gcatggacgg
      661 cctggcaatt atgggagcta ctttcgtgcc gcatcgcgcc aaactcgccg aacttgcaat
      721 gacagcgcga ttgccggtca taacgttcct cagggagttc actgaggccg gcgccctgtt
      781 gagctatggg gtgaacatgt ccgatttcat tgggcgtgct gcggtatacg tggaccggat
      841 tctcaaaggc gcgcgccccg ccgatctcgc agtcgagcag cccacaaagt tcgaactggc
      901 catcaaccta aggaccgcga aagcgctcgg cctctccgtg ccgccggtgc tgctcgccac
      961 ggccgacgag gtgatcgaat gaccgcttgt ggcacttttc ggaagtgccg caatgtccgc
     1021 cttgagtccg gaatgcgcgc caaagccgac gtccgccgac cactcggaat ttatgggttg
     1081 gcgccctagt cgtccgaggt cggtccgcac cagcccggca aatagaccgc gtccctgctt
     1141 ttcgccaggg ccagcgcttt ttcgatggcc ttgtcgaagt tggcctccac gctcttgcgc
     1201 gataaccggc ggcgcaggaa atccgcccaa aggaattcgc tgaacggtgt tgtgtccttg
     1261 gcgaagccgc cgacgcgtcg caattcgccg gcaaggctgc ggaatggatc gtccttgagc
     1321 tccactaccg tttttggaag gtttctgaag tgctggcgct ggcccttggc atcataggga
     1381 taggcccacc tgtgattgtc gagcacgccc cagaatgcgt cgcggtcgac catggtgaga
     1441 tcggcgatga cggtgacgat aatatcctcc acgccttcgt cgtgaagcgc gcgcgccaga
     1501 tggtgatgat cgaccacgta gtggcgctgg tccggcccaa gcaccaccgg gatcatatgt
     1561 ctgcccagaa actggccctg ctttatcttc ttgtgcttcc gccagtgctc ccgcaggcgc
     1621 ttgcgctttt ccttcacctc tctcattcca accgtcatct gggttggatg cagcgagagg
     1681 atcggaatcg gctgaagaat cggttcacgc gtattcgcca tggtttgaaa ccccttgttt
     1741 gaccgccaaa aatagctctg aaattcctcc ggttatgtca tgatggcacg ggcgatcaaa
     1801 tgcctgtgcg ccctcaagag attttgaaag atgatcaaag gccggagcga gcgaacagcg
     1861 aacaagcggg tcgtgctcct tggcggccgc gaggtcatcg ccgaaggcgt ccgtctgaat
     1921 ttttgggccg atataagcca tcgctgcatg acagcctcat ggccgtcttt cattgccgga
     1981 gccgcgctgg tgttcgttgt tttcaacgcg gtgttcgcag tgttctactg gatcggaaat
     2041 cagccgatct cgaatgtgcc cgacggcgcc tatatcgatt atctctattt cagcatcgag
     2101 acgctttcga ccgccggtta cggcgacatg catccgcaga cccattatgg acatttcatc
     2161 gcgacggtgg agctcttcac cggcatcttt tcgatgtcgc tgatgaccgg gctgatcttt
     2221 gcacggttct cgcggccaaa tgcccgcttg ctgttcgccg accacccggt gatttcaagc
     2281 catgagggca agccgaccct gatgatccgc ttcgccaacg agcgccacga catcatcggc
     2341 aacgccaccg caaggctgtg gctgctcaag aacgtcgtga ccactgaggg ccaatcgctt
     2401 cgaagattct acgagctttc gctggtgcag aatgagcacc ccgcgctggc cttgagctgg
     2461 acgctctacc atattctcga tgagcaaagc ccgctctacg ggctcaatgc cgacgatctt
     2521 gccgcatcgg acgtttcgct ggtggttgtg gtgtccggat acgacgtcgt cgcggcgcag
     2581 atcgtccacg cgcgaaaatc ctacgattat cggcatattc gttttgggc
//
LOCUS       DASTPM010000343         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1222885, whole genome shotgun sequence.
ACCESSION   DASTPM010000343 DASTPM010000000
VERSION     DASTPM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222885"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFR42_06005"
     CDS             <1..801
                     /locus_tag="VFR42_06005"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEU0368740.1"
                     /translation="LTGHATARDATSQTSAQKLTFWQATGDVRGEGNVRSSDLSARNT
                     TVHLAPAASNISADNLTGNARTGSALYSGHARLWQGDSVMEAESIELLKNERMLNAAG
                     NVRAAFPQASSNANAVQNQTAKSLKAPTLWHAQAAKLTFWDADNRAHLSQNVVVQAPD
                     QKMSGQDLDLYFTRANGAGQGAQQISRAVGSGGVTVVQGDRRATAERGEYTAADGKFV
                     MTGGTPTIFDASEGSTTGRQLTFFLADATIIVDSENGSRTLTKHRVEK"
     gene            867..1592
                     /gene="lptB"
                     /locus_tag="VFR42_06010"
     CDS             867..1592
                     /gene="lptB"
                     /locus_tag="VFR42_06010"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU0368741.1"
                     /translation="MLKLQAVELHKSYRGRRVVDDVELEIGQGEVVGLLGPNGAGKTT
                     TFYILVGLARPDSGRVLLNGEEITDLPMYLRARSGISYLPQEPSVFRQLTSEENLLAV
                     LETLPLTPEQQRDRLEELLVQMGLETVRTSKAYTLSGGERRRLEIARSLTLEPSFILL
                     DEPFSGIDPLTVKDLQEIIRELAKSGIGVLITDHNVRETLTVTDRAYILKSGKIFRKG
                     RPEELSNDPEVRRVYLGDHFRLN"
     gene            1642..>2814
                     /gene="rpoN"
                     /locus_tag="VFR42_06015"
     CDS             1642..>2814
                     /gene="rpoN"
                     /locus_tag="VFR42_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU0368742.1"
                     /translation="MAWQGLKLNLKVAQKQILTPGLVQMVSVLALNRLELREMINAEV
                     VANPVLEELSEEPTTSDNYSDENFLKNETEKVPEKPESNPFDEFDVGTFFNQYLDTGG
                     DGGKSQEREISERPSFEKFLSSPAGLTEHLTWQLSVTICSDAVREIAESIIGNLDENG
                     YLAASQEELLQEGKYTQDDLEDAVAVVQDFDPIGVGARDLRECLLLQLKAFDPQNTLA
                     QQIVSEHLKQVQSNQLKEIARAVNRPIEVVKRTLDTIKKLDPRPGLRYNKTEPRLVEP
                     DVYFRKAEGAWQVFLNEDDMPQLRLSPTYRRLLARDAADRDVRNYVKERFTAAIQLLK
                     NIEQRKHTILRVCQSIIARQGEFLDHGPDALKPMMIKEVAEEVGVHPSTVSRAVASK"
BASE COUNT          666 a          738 c          881 g          529 t
ORIGIN      
        1 ctgaccggcc acgctacggc ccgcgatgcg accagccaga cttcggcaca gaagctgacg
       61 ttttggcagg cgacgggaga tgtgcgcggc gaaggtaacg tgcgctcgtc tgatctctcg
      121 gcgcgcaaca ccaccgtgca cctagcgcca gctgcttcaa acatcagcgc ggacaatttg
      181 acgggcaacg ccaggacggg tagtgcgctc tacagcggcc acgcgcgctt gtggcagggc
      241 gattcggtga tggaagccga atcgatcgag ctcttgaaaa acgaacgcat gctgaacgcg
      301 gcgggaaatg tacgagccgc ttttccgcaa gcatcgagca atgcgaacgc agtgcaaaac
      361 caaaccgcga aatcgctgaa ggcaccgacg ctgtggcacg cgcaggcggc caagctgact
      421 ttttgggacg cggacaatcg tgcccacctt tcgcagaacg tcgtggtgca ggcgcccgac
      481 cagaaaatga gcggccaaga tctggatttg tatttcaccc gcgcgaatgg cgcggggcag
      541 ggcgcgcaac aaatcagccg cgcggtgggt agcggcggcg taaccgtggt gcaaggcgat
      601 cgcagagcaa cggccgagcg cggcgaatat accgcagccg acggaaaatt cgtcatgacc
      661 ggcggaacac ctacaatttt tgacgccagc gagggcagca cgaccggccg tcaattgacc
      721 ttctttttag cggatgctac gatcatcgtg gattctgaaa acggatcgcg cacactgacg
      781 aagcaccgag tcgaaaagta acggtgcggg ttctatctgg agcccgcaga aacaagtggc
      841 ctctggcggt cacgacgtga cgaggaatgc tgaagctcca ggccgtcgaa ttgcacaagt
      901 cgtatcgcgg tcgcagggtc gttgacgacg tagaactgga gatcggacag ggtgaagtcg
      961 ttgggctctt ggggcccaat ggcgcgggca agacaactac tttttatatc ttagtgggtc
     1021 tggcccggcc cgattccggc cgcgttttgc tgaacggcga ggagatcacc gatctcccga
     1081 tgtaccttcg ggcccgcagc ggcatcagct accttccaca ggaaccttcg gtctttcggc
     1141 agcttacgag cgaagagaat ctactggcgg ttctggaaac cctccctctc actccggaac
     1201 aacagcgcga ccgtttggag gagttgttgg tgcagatggg actggaaacc gtgcggacca
     1261 gcaaggccta cacactgtcg ggcggcgaga ggcggcggct ggagattgcg cgatcgctga
     1321 cgctggagcc ttccttcatc ctgctggatg agcctttttc cggaatcgat ccgttgactg
     1381 tcaaggattt acaggaaatt attcgtgagc ttgccaaaag cggcatcggc gtgctcatca
     1441 cggatcataa tgtacgagag accttgacgg tgacggaccg cgcgtacatt ctgaaaagcg
     1501 gcaagatttt tcgcaaaggg cgtccggagg agttgagcaa cgatccggaa gtccggcgcg
     1561 tgtatctggg cgaccatttc cgcctgaatt agggtttacc attaccgagg agcagcagga
     1621 agggacgatt tttcggcaac catggcctgg cagggactca aactcaatct gaaggtggcg
     1681 cagaagcaga ttctgacgcc cggcctggtg cagatggtca gcgtgctggc gctgaaccgc
     1741 ctggaattgc gtgaaatgat taacgccgaa gtggtggcga atcccgttct ggaagagctt
     1801 agcgaagagc ccaccaccag cgacaattac agcgacgaaa acttcctgaa aaacgaaacg
     1861 gaaaaagtgc cggagaagcc ggagtcgaat ccgtttgatg aattcgatgt cggcactttc
     1921 tttaatcagt atctggacac gggcggcgat ggcggcaagt cacaagagcg ggaaatttcc
     1981 gagaggccgt cgttcgaaaa gttcttgtct tcgccggcag gattgaccga gcacctcacc
     2041 tggcaactca gcgttaccat ttgttccgac gcggtgcggg aaatcgcgga aagcatcatt
     2101 ggcaatttgg atgagaacgg ctatctggcc gcgtcgcagg aagaactatt gcaggagggg
     2161 aaatacacgc aggacgatct ggaagatgct gtcgcggtgg tgcaagattt cgaccccatc
     2221 ggggtaggtg cgcgggactt gcgcgaatgc ctgctgctgc agctgaaggc gtttgatccg
     2281 cagaatacgc tggcacagca gattgtttcc gaacatttga aacaagtgca gtccaatcag
     2341 ctgaaggaaa ttgcgcgcgc ggtgaatcgt ccgattgaag tggtaaagcg caccctggac
     2401 acgattaaga aactcgatcc gcgaccggga ctgcgctaca acaaaacgga accacggctg
     2461 gtggagccgg acgtctattt tcgcaaggcg gaaggcgcct ggcaggtttt tctgaatgaa
     2521 gacgacatgc cgcagttgcg cctgagcccg acgtatcgcc ggttgctggc gcgcgacgcg
     2581 gccgaccgcg atgtgcgtaa ttacgttaag gaaagattca cggcggcgat tcaactgtta
     2641 aagaacatcg agcaaagaaa gcacacgatt ctgcgcgtgt gccagtcgat cattgcgcgg
     2701 cagggcgaat ttttggatca cgggcccgat gcactgaagc ccatgatgat taaggaagtg
     2761 gcggaagaag tgggagtgca tccatctacc gtgagccgcg cggtggccag caaa
//
LOCUS       DASTPM010000344         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_675337, whole genome shotgun sequence.
ACCESSION   DASTPM010000344 DASTPM010000000
VERSION     DASTPM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675337"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFR42_06020"
     CDS             <1..346
                     /locus_tag="VFR42_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0368743.1"
                     /translation="PATPKTELLQGTLDMLILRTLLFGPAHGHQIAKHIQRTTVDVLQ
                     VEHGSLYPALHRMERKGWISAKWEQAKDRNREFKYYRLTPAGRKQVAHEESKWKKLAA
                     AVARVMWPVREE"
     gene            349..3051
                     /locus_tag="VFR42_06025"
     CDS             349..3051
                     /locus_tag="VFR42_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368744.1"
                     /translation="MKWNWFASRVAGLRPNQRRMADVQRELQADLELEAEEQRESGLS
                     PEESRYAARRAFGNTTLITEDVRAVWTFAWLEGFLRDLRYAARALRKNLGFTAVAVLT
                     LALGIGANTTIFSALNALLLQPLPFPAAGELVRIYSTKGASSSSPTLVGPSAMDVRDF
                     AQSSHTFQRMTVYDTWRKNVSFGDSGVEPEQMRVGLVPAAYFEILGVQPIAGRLFTDA
                     ENQQGRNYVAAISARVWNTRFSGDRAILGRKLRINDEAYTIIAVMPDVIPEWMEPWRP
                     GLIEIWTPFLDAWTETSRAARGNAAIGRMKAGVSLEQAQKDLSTIAAALSEAHPVNRG
                     IGVAVAQLADTRAGKLRPMLFLLMAAVSLILLIACVNLANLLLARNSSRQRELAVRAA
                     LGAGRGGLVRHLLAETLLLSLTGAVFGLALAKVALAALTRMHPPGLPQLDSLGLDWRV
                     LAFTLVVSLVTSLLFGLTPALYGTRLNLVDSLKQGGRSGTSGPGSQRLRNALVVTEMA
                     MSLMLLVGAGLLAQSVLRLEHQSLGIRQDHLLTGHFYLPGVRYPDGGAITRFCDEFTR
                     RVRGLPGVISASVTTAYPPNNGWTQMLGIPGRPLTRIEDVPTAQFGVTDSSFLATMGI
                     PLLRGRDFSEADNATSLPVALISEEFRRRYFPNEDPLGRQIHIGPPPFLQLPAGANTT
                     DSADVTIVGVVGDFRNAGLVLPPEPHITVLYSQHPLVNYGFKDIVIRTAAQPHSLAPE
                     IRRQLHQLDADMPFAEVQTIDELVERQTGGQRFTTALLGAFAVSGLALAGVGIYGVVS
                     FLVAQRRQELAVRMAMGASRATVMWLVLKKSLTMAAVGAAAGLLGAVSAQKLTSGLLF
                     GVSPVDPITFAGAAAFLLLLAGVASAIPGARVMRIDPALALRQD"
BASE COUNT          575 a          938 c          949 g          621 t
ORIGIN      
        1 acctgccacc ccaaagaccg aactgctgca aggcaccctc gatatgctca tcctgcgcac
       61 cttgctcttc ggacccgctc acggccacca gatcgccaag cacatccaac gcaccaccgt
      121 ggacgtcctg caagtcgagc atggctctct ttacccggct ctccatcgca tggagcgcaa
      181 gggctggatc agcgcgaagt gggagcaggc caaggatcgc aatcgcgagt tcaagtatta
      241 ccgcctgaca cctgcgggaa gaaaacaggt ggcgcacgaa gaatccaagt ggaagaaact
      301 cgccgcggcg gtggcgcggg ttatgtggcc ggttcgagag gagtagtcat gaagtggaat
      361 tggttcgctt cgcgcgtcgc cggcctgcgg ccaaatcaac ggcggatggc cgacgtgcaa
      421 cgcgagctgc aagcggacct cgaactcgaa gccgaagagc agcgggaatc tggattgtcg
      481 ccggaagaat cgcgctacgc agcgcgccgg gccttcggca acacgacgct aattaccgag
      541 gacgtgcgcg ccgtatggac tttcgcctgg cttgagggtt ttcttcgcga cttgcgctac
      601 gccgcgagag cgctgcgcaa aaacctggga tttaccgccg tggccgtgct cacactggcc
      661 ctcggcattg gcgccaacac caccatcttc agcgccctaa atgcgctcct gctccagccg
      721 ttgccgtttc ccgcggccgg cgaactcgtg cgtatttact cgacgaaggg agctagcagc
      781 agctcgccca ctctggtcgg gccctccgca atggatgtgc gggactttgc gcagagcagc
      841 cacacctttc agcggatgac ggtgtatgac acctggcgca agaacgtaag cttcggcgac
      901 tccggagtgg aaccggaaca gatgcgtgtc ggcttggtgc cagccgccta tttcgaaatc
      961 ctcggggtgc aacccatcgc cgggcggctc ttcaccgacg cagaaaacca gcagggcaga
     1021 aactatgtgg ccgccatcag cgcgcgcgtg tggaacaccc gcttctccgg cgatcgggca
     1081 attcttggcc gcaagctgcg catcaatgac gaagcctaca ccatcattgc cgtaatgccg
     1141 gatgtgattc cggaatggat ggaaccctgg cggcccggtc tgatcgaaat ctggacgcca
     1201 tttctagacg cctggacgga aacttcgcgg gccgcccggg gaaacgcggc gatcgggcgg
     1261 atgaaggccg gcgtttcgct cgaacaggca caaaaggatt tatccacgat tgccgcggcg
     1321 ctctcggaag cacacccggt gaatcggggt atcggcgtgg cggtagcgca gcttgccgat
     1381 acacgcgctg gaaagctgcg cccaatgctt tttcttttga tggcggccgt aagtttgatc
     1441 ctgctgattg cttgcgtgaa tctggcaaat ttgctgctgg cgcgaaattc atcgcgccaa
     1501 cgcgagctgg cggtgcgcgc cgcgctgggc gcgggcagag gcggcctggt aaggcacttg
     1561 ctggcggaga ctctactcct gtcgctcacc ggagcggtat ttggtttggc gctggcaaag
     1621 gttgcactcg cggccctcac cagaatgcat ccgccgggtc tgccgcagtt ggattcgctc
     1681 gggctggatt ggcgggtgct ggcatttacc ctggtcgttt ctctcgtgac cagccttttg
     1741 ttcggtttga cccccgcgtt atacggcact cgcttgaacc tcgtcgattc cttgaagcaa
     1801 ggtggccgct ctggcacttc cgggccgggg agtcagcgct tgcggaatgc actggttgtt
     1861 acggaaatgg ccatgtcgct gatgcttctg gtcggcgcgg gattgctggc gcaaagcgtc
     1921 ttgcgtttag agcaccagag cttgggaatc cgtcaagatc acttgctgac cggacatttt
     1981 tatctgcccg gagttcgcta tccggatggc ggcgctataa cgcgattctg cgatgaattt
     2041 acgcggcgcg tccgcgggtt gcccggtgtt atctctgcga gtgtgaccac cgcgtatcca
     2101 cccaataatg gctggacgca aatgctgggt attcccggac gcccgcttac gcgtatcgaa
     2161 gacgttccca ccgctcaatt cggcgttacg gatagcagct ttcttgccac catggggatt
     2221 cccctgttgc gtggccggga tttttcggaa gcggacaatg cgacgagcct tcccgtggcg
     2281 ctaatcagcg aggaatttcg ccggcgttat ttccccaacg aagatccgct cggccgccaa
     2341 atccacatcg ggcctcctcc gttcttgcag ttacccgcgg gagcaaacac cacggactcc
     2401 gcggacgtga ccatcgtggg agtggtcggt gacttccgga atgccggcct ggtgcttccg
     2461 ccggagccgc acatcactgt tttgtactcg caacatccgc tggtgaatta cggcttcaag
     2521 gacattgtga ttcgaacggc cgcgcaaccg cattccctgg cgcctgaaat ccgcaggcaa
     2581 cttcatcaac tcgatgcgga catgcccttc gccgaagtgc aaaccatcga cgagctggtg
     2641 gaacggcaaa ccggcgggca acgctttacc accgcgttgc tgggggcatt tgcagtgtcc
     2701 ggcctggcgc tggccggcgt tggaatctat ggagtggtgt cctttttggt cgcgcaacgc
     2761 cgccaggaac tagccgtgcg catggccatg ggagcgagcc gggcgacagt gatgtggctc
     2821 gtactcaaga aaagcctcac catggccgca gtcggcgccg ccgccggact tctgggcgca
     2881 gtgtctgcac aaaaattaac gagcggcctt ctcttcggag tttcaccggt cgatcccatc
     2941 acgttcgcgg gggccgcggc ctttcttttg ctcctggccg gagttgccag cgccatcccc
     3001 ggcgcgcgag tgatgcgtat cgatcctgcg ctggcgcttc gccaggattg agggcccaaa
     3061 ggaaacccgc aaacgcgtcg gcg
//
LOCUS       DASTPM010000345         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1367134, whole genome shotgun sequence.
ACCESSION   DASTPM010000345 DASTPM010000000
VERSION     DASTPM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367134"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..547)
                     /locus_tag="VFR42_06030"
     CDS             complement(230..547)
                     /locus_tag="VFR42_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368745.1"
                     /translation="MSPKRLLMTALVTVTVLVASAFAQKNELTGIIGRTFISDQGVKN
                     VNLFNNNVHAGAGLTFEVNYGRHLMGEISGASHLKCRRCSISTKISTSPPIRSRKVTN
                     RFS"
     gene            713..2203
                     /gene="rimO"
                     /locus_tag="VFR42_06035"
     CDS             713..2203
                     /gene="rimO"
                     /locus_tag="VFR42_06035"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU0368746.1"
                     /translation="MPKPNTTVALLDGEQTDLLDKPRPKKVGFVSLGCPKNLVDSEVM
                     MGMLTQAGAELTQRAEDAEVIVVNTCSFIESAQQESVNTILEMAKHKVDGRARKLVVA
                     GCLVERYRDEIRKNIPEIDAVVGTGELERILAAAGVAPAHDPNSPFKILTSRPEGDAR
                     EAAGRFSRDRWDGAIADLPNYLYDETTPRVLATASHTAYIKIAEGCDHPCTFCIIPQL
                     RGKFRSRRFESVIAEAEQLARRGVREVTLIGQDTTCYGEDFGLKDGLALLLEKLARIE
                     DLRWVRFLYAYPNKITGRLLETIAAHEKICSYVDVPLQHASPAVLKRMKRGGGADVFL
                     RTIEKMRRTIPDLTLRSSFIVGFPGETEKEFEELCDFVREAQLDWMGAFSYSDQEGAA
                     AYGQDKKVPVKEIERRRKRLMQIQRQISKKRKKALVGKEFDLVLEGTSEETDLLLEGR
                     TAMHAPEIDGKVLVNDLPEGVAPEFGKFYRCEVTEAHEYDLVARIV"
     gene            2243..2893
                     /locus_tag="VFR42_06040"
     CDS             2243..2893
                     /locus_tag="VFR42_06040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0368747.1"
                     /translation="MGGLIPLGDASRRPASIPVVTGCIIFANICVFVLELIRGDAFVL
                     QWSAIPAQITSGHHLITILTAMFMHGSWSHIIGNMVFLWAFSPEIEGAMGRGRYLAFY
                     LAGGVVAMLAQVLADPHSTVPNLGASGAIAAVMGAFMVTYPRDQIRSLLIIFIFVRVT
                     FIPAAVLIGFWFITQLVHAGTVAHSQTGGVAYLAHVGGFIFGAVTAHWFERQNQME"
BASE COUNT          622 a          789 c          996 g          635 t
ORIGIN      
        1 ggcgttcccg accagaagtc gcgggcgcct cctcgcaggc tgaaatgcgg cgtgaggcgg
       61 acgtcgagtc cgacgccgat ctgcatcact ccggtggtcg aggatttcgc gccgctgggg
      121 ccgccgaact cgagttgatc cgacgggctg aaccgcccga ttcctccgcc gaagcttacc
      181 tagggcgaga ttgctgtcgt ggcgaaagca ttcgcgcgca gcgacggcgt cacgaaaaac
      241 gatttgtaac tttccgggat cgaattggcg gcgaagttga gatcttcgtc gaaattgaac
      301 accgccggca cttcaaatgt gaggcgccag aaatttcccc catcagatgg cggccgtagt
      361 tgacctcgaa ggtcagccct gctccagcgt gaacgttgtt gttgaacagg ttcacattct
      421 tcacgccctg gtcgctgata aaggttcgtc cgatgatgcc cgtgagttca ttcttctgag
      481 cgaaagcgct ggccaccagg acggtaactg tcaccaaggc tgtcatcaaa agccgtttgg
      541 gggacatctc tcctccagag cacggctgaa ctgtttcgat tatggctcgg gataagttag
      601 cacgccgtcc gccatttcac gaccaaggct tgctgctttg ttggataccc gctgtccgga
      661 atgggagggt cgccaaagcg gtgcaaggcg gatgttaaaa taatgagatc gcatgcctaa
      721 accgaacaca actgtagcgc ttctcgacgg cgaacagacg gatttgctag acaagccgcg
      781 tccgaaaaag gtggggtttg tcagcctggg gtgtccgaag aatctggtgg attccgaggt
      841 catgatgggc atgttgaccc aggcgggcgc cgagttgacg caacgcgccg aggacgcgga
      901 agtcatcgtg gtgaacacct gctcgttcat cgagagtgcg cagcaggagt ccgtgaacac
      961 catcctggag atggcgaagc acaaggtgga tggccgcgcg cggaagctgg tggtggcggg
     1021 gtgcctggtg gagcggtatc gcgacgaaat ccggaagaac attccggaaa tcgacgcggt
     1081 ggtgggtacg ggggaattgg agaggatttt ggctgcagcg ggcgtggccc cggcgcacga
     1141 tccgaattca ccgttcaaaa ttctgacttc gcggcccgag ggagatgcgc gggaagccgc
     1201 cggccgtttt tcgcgggacc ggtgggacgg cgccatcgcc gacctgccca attatctcta
     1261 cgacgagaca acgccgcgcg tgctggcgac agcttcgcat accgcgtaca tcaagattgc
     1321 tgaggggtgt gatcatccgt gcacgttctg catcattccg cagctgcgcg gaaagtttcg
     1381 gtcgcggcgg tttgagtcgg tgatcgccga ggcggaacaa cttgcgagac gcggagttcg
     1441 cgaggttacg ctgatcgggc aggatacgac ttgctacggc gaggacttcg ggctgaagga
     1501 tgggctggcg ctactgctgg agaagctggc gaggatcgag gacctgcgat gggttcggtt
     1561 cctgtacgcg tatccaaaca aaatcacggg acgtctgctg gaaaccatcg cggcgcacga
     1621 aaagatctgt tcgtatgtgg acgtgccatt gcagcatgcg tccccggcgg ttctgaagcg
     1681 gatgaagcgg ggtggcggag cggatgtctt tttgcgcacg atcgagaaga tgcggcggac
     1741 gattcctgat ctgacgctgc ggagctcttt tatcgttgga tttccaggcg agacggaaaa
     1801 ggaattcgag gagctgtgcg actttgtgcg ggaggcgcag cttgactgga tgggggcctt
     1861 cagctactca gaccaggaag gcgccgccgc gtatgggcag gacaagaagg ttcctgttaa
     1921 agaaattgag cggcgacgca agcgcctgat gcagattcag cggcaaatca gcaagaagcg
     1981 gaagaaggcg ctggttggaa aagaattcga tctggtgctt gaaggaacgt cggaagaaac
     2041 ggatttgctg ctggaagggc gtacggcgat gcacgctccg gaaattgatg gcaaggtgct
     2101 ggtgaatgac ttaccggagg gcgtggcgcc ggagttcggg aagttttacc ggtgcgaggt
     2161 tacggaagcg cacgagtacg atttggtggc gaggatcgtt tagcatccag gcacatttct
     2221 ccgcgaatgg aggaaaaacg aaatgggagg cctgatcccc ctgggcgatg cgtcaagacg
     2281 cccggccagt attcccgtcg tcactgggtg catcatcttc gccaatatct gtgtcttcgt
     2341 gcttgagctg attcgtggcg acgcatttgt gttgcagtgg tccgcaattc ccgcccagat
     2401 tacttcgggg catcacttga tcacaattct gactgccatg ttcatgcacg gcagttggtc
     2461 gcacatcatc ggcaacatgg tcttcctgtg ggcttttagt cccgagatcg aaggggccat
     2521 ggggcggggc cgctatttag ctttctacct ggctggcggg gtggtggcga tgctggcgca
     2581 ggtcttggcc gacccccact cgacagtccc gaacctgggc gcaagtggcg ccatcgcggc
     2641 agtgatgggt gcgttcatgg tcacctaccc acgcgaccag atacgctcgc ttctgatcat
     2701 ctttattttc gtgcgtgtca catttatccc cgcggcggtc ttgatcggct tctggtttat
     2761 tacgcagctt gtgcacgccg gcactgtggc gcactctcag accggcggtg tggcctacct
     2821 ggcacacgtc ggcggcttca tttttggagc ggtcaccgca cactggttcg aacgtcagaa
     2881 ccagatggag tgagtccggt gaagtcgaga tccaccatgc ccaaacgcct caaactgaaa
     2941 tgcccgatct gcaagaagcc ggtgaagagc gcggacccgg agtttccttt ctgcagcgaa
     3001 cgttgccgaa cgattgatct gggcaagtgg gcgagtgggg ga
//
LOCUS       DASTPM010000346         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_156023, whole genome shotgun sequence.
ACCESSION   DASTPM010000346 DASTPM010000000
VERSION     DASTPM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156023"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VFR42_06045"
     CDS             <1..2083
                     /locus_tag="VFR42_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0368748.1"
                     /translation="LQLLINDRAFSFTVRGRFPDDAGAGSAILMDIAAAQTVLGRVGH
                     VDRIFIRLPKSTTASLDEWQRRVEQVLPKGAQLRAAGASTDENRKMLAAFRWNLRLLS
                     YIALVVGAFLIYNAISVSVVRRRAEIGIVRALGASRGQVLLAFLLEAAVIGGAGALAG
                     VPLGRFMASSAVKLMGNTVNALYVSSRPGEIALGVASILLALFSGLFVTLLSAWSPAR
                     EAASIAPVEAMARGRREYSVRVNKKFALCIATALAATAAAAARLPPVGGKPIFGYCAV
                     LLLVAAGAFAIPAFAEIAMRASAGVFSKLLGVEALLASRSLTGSLRRTSVLVSALCTA
                     VSMMAAVAIMVGSFRQTVVLWMASELPADLYLRPAGNPATDQHPTISPAFSEKLARLP
                     GVRAMQRLRAYEISYQGKPATLASVDLDDPHIEHSSDFLSGRSAENVLPELRGANAVI
                     ISEPFSYKHHVNRGDTIPLPLGDENATFRIADVYYDYASERGMILMDRNTLLKYLPDP
                     APSNIAVFVSPGVNPANVRAEIERAAAAADARILIFANSDLRKQAVQIFDRTFAITYA
                     LEAIAVFVAIMGVAGALLALVIDRRRELGLLRYLGASSLQIRKMILAEAGLLGLLSNI
                     AGLCLGIFLSLILIFVINKQSFGWTIRLHWPIAVLLAGISVIWIATLLAGCYPARIAV
                     RLNPLEVIHED"
     gene            2073..>2678
                     /locus_tag="VFR42_06050"
     CDS             2073..>2678
                     /locus_tag="VFR42_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HEU0368749.1"
                     /translation="MKTELRALYALALSVFFPISVSIAQFQTALPGYHYEFPRDYFDH
                     PDFQTEWWYYTGNVQSADGHKFGFELTFFRQGLSRDPKQNSSWDLRDIYFAHFALSDL
                     TGQKFFHKERSNRPGPGIAGASASLQKVWNGNWSVVWVNGEERLTALADSFVLNLSLY
                     SQKPQVAHGVNGVSQKSAGTGRASHYFSETRLAASGSLTFYA"
BASE COUNT          459 a          900 c          715 g          604 t
ORIGIN      
        1 cttgcaactc ctcatcaacg accgcgcttt ctcgttcacg gtgcgcggta ggtttcccga
       61 cgacgctggc gctggctcgg ccatcctcat ggacatcgcc gcagcgcaaa cagtattggg
      121 gcgcgtcggc cacgtcgacc gcatcttcat ccggcttccc aaaagcacca ctgcttcact
      181 cgatgaatgg caacgccgcg tcgagcaggt tctacccaaa ggcgcgcaac tgcgcgccgc
      241 cggcgcatcc acggacgaaa accgcaaaat gctcgccgca tttcgctgga atctgcggct
      301 gctcagctac atcgccttag ttgtcggagc cttcctcatt tacaacgcca tctccgtctc
      361 cgtggtccgg cggcgcgcgg aaattggcat cgtccgggcg ctcggcgcca gccgcggcca
      421 ggtgctcctc gcgtttctcc tcgaagctgc ggtcatcggc ggcgccgggg ccctggccgg
      481 tgttcctctc ggccgcttca tggcctcatc cgccgtcaag ctgatgggca acaccgtcaa
      541 cgcgctttac gtcagcagcc ggccgggcga aatcgccctg ggcgtagcat ccatcctgct
      601 cgcgcttttt tctggtttgt tcgtaacgct gctttccgcc tggtcgcctg cccgcgaagc
      661 cgccagcatc gctcccgtgg aagccatggc ccgcggtcgc cgcgagtatt cggttcgcgt
      721 aaacaagaag ttcgcgctgt gcatcgccac cgcccttgcg gcaacagcgg ccgccgcagc
      781 gcgcctgcct ccggttggcg gcaaaccgat ctttggctac tgcgcggtgc tcctcttggt
      841 ggcagccggg gcattcgcga tccccgcttt tgcggaaatc gccatgcgcg catcggcggg
      901 cgtattttcc aagttgctcg gcgtcgaagc gctcctcgcc tcccgtagcc tcacaggttc
      961 gctgcgtcgc acttccgtcc tcgtctcggc tctctgcacc gctgtcagca tgatggccgc
     1021 tgtcgccatc atggtcggca gttttcggca aacggtcgtc ctttggatgg cgtccgaact
     1081 tcccgccgat ttgtacttac gtccggccgg aaatccagcg accgatcagc acccgacgat
     1141 ttcaccggcc ttctccgaga agcttgcgcg gcttcccggc gttcgcgcca tgcagcggct
     1201 gcgcgcctac gaaatcagtt accaaggcaa acccgccact ttggcctctg tcgatctgga
     1261 cgacccgcac atcgaacaca gttccgattt cctgtccggg cgctctgccg agaatgttct
     1321 gcccgagttg cgcggcgcaa acgccgtgat tatcagcgaa cccttcagct acaaacacca
     1381 cgtaaatcgt ggagacacca tcccactgcc gctgggcgac gaaaacgcca cattccgcat
     1441 cgcggatgtc tactacgact acgccagcga gcgcggcatg attctgatgg atcgcaacac
     1501 gctgctgaaa tacctgcccg accccgcgcc ttccaacatc gctgtttttg tttctcccgg
     1561 cgttaacccc gccaatgttc gcgcggaaat cgaacgcgcg gcagcagcag cggacgcgcg
     1621 catcctcatc tttgcgaata gcgacctgcg caaacaggcc gtgcaaattt tcgatcgcac
     1681 ttttgccatc acttacgctt tggaagccat cgctgttttc gtggccatca tgggtgtcgc
     1741 gggcgctttg ctggcgctgg tgatagaccg gcgacgcgaa ctcggtctgc tccgctatct
     1801 cggcgcatcc agccttcaaa ttcgcaaaat gattcttgcc gaagcgggct tgctcggtct
     1861 gctttccaat atcgcgggcc tgtgtctggg aatttttctc tctctgattc tgatctttgt
     1921 catcaacaag cagtcgttcg gctggactat ccgcttgcac tggccgattg cggttctcct
     1981 ggcagggatt tcggtcatct ggatcgcgac gctccttgcg ggctgctatc ccgcccgtat
     2041 cgccgtccgg ctaaatccgc tcgaggtcat ccatgaagac tgaacttcgc gcgctctacg
     2101 ccttggctct gtccgtgttc tttcctattt ccgtgtccat cgcccaattt caaaccgcgc
     2161 tgcccggtta ccattacgaa ttcccgcgcg actactttga tcaccccgac ttccagaccg
     2221 agtggtggta ttacaccggc aacgtgcaga gcgccgacgg tcataaattc ggtttcgagc
     2281 tgactttctt ccgccaggga cttagccggg atccgaagca aaattcttcg tgggatcttc
     2341 gcgacatcta tttcgcgcat ttcgcgctca gcgatctcac aggccaaaag ttcttccaca
     2401 aggaacgcag caatcgccct ggcccgggca tcgcgggcgc cagcgcctct ttgcaaaaag
     2461 tttggaatgg aaactggagt gtggtgtggg tgaacggcga agaacgcctt actgccctgg
     2521 ccgattcttt cgttttaaat ctctccctgt attcgcaaaa gccgcaggta gctcacggcg
     2581 taaacggcgt cagccaaaaa tctgccggga ccggccgcgc ttcgcattac ttctcggaaa
     2641 ctcgcctcgc cgcctccggc tctctcacct tttacgca
//
LOCUS       DASTPM010000347         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_675392, whole genome shotgun sequence.
ACCESSION   DASTPM010000347 DASTPM010000000
VERSION     DASTPM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675392"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VFR42_06055"
     CDS             <1..1383
                     /locus_tag="VFR42_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU0368750.1"
                     /translation="LILLLLGWVFVPFYLRSNVFTMPQFLERRFSRQCAVYLAGISII
                     AYIFTKISVQLYAASVVLERVAGWSLWKTAVVLVIATGIYTIAGGLAAVIYTDTVQTL
                     ILITGAVALTLIGLDKVGGIGHLRTMVPDSYFHMMKPMSDPQFPWTGIFFGAPILGIW
                     YWCTDQVIVQRVLSARDEGHAKAGTIFAGFLKILPVFMLVLPGIIAYALYPAEVAKPD
                     FAYPTLVLNLLPVGLVGLVMAALLAAVMGAMSSVFNSASTLVTLDFYKKLRPQASEAQ
                     LVNFGRIATGGMVLLGLLWVPFIHLLSAQLYIYLQSVQAYISPPIAVCFIFGILWTRV
                     NGQGAISSLLTGFVLGALRFVFEVLDKTRHYESAFVRWLVDMNFLHYAIFMFVVCTAV
                     LIGVSLMYPAPDRKKLAGLTFATVDDKIDSAEVARPHLAKETSTEHAINVALTLLLLA
                     TVVGLWIYFR"
     gene            1406..1954
                     /locus_tag="VFR42_06060"
     CDS             1406..1954
                     /locus_tag="VFR42_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368751.1"
                     /translation="MKSIRSLRPNVTREEAIRMFSAPGPASFLWKLRSGPLQRIADAY
                     IPFNLYRVAYHMGGAPRSHLFALDAVDGSLDLFQFGAAPNDAETVHVSTRNHPAAKLS
                     SSKAEELLREKVLRLIFQQGFFKVRAVKLEIERLPGEIHLPYWLGFYGTQENLRCRVM
                     DAVRRRVEGARASALFEQWLAA"
     gene            1989..>3900
                     /locus_tag="VFR42_06065"
     CDS             1989..>3900
                     /locus_tag="VFR42_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEU0368752.1"
                     /translation="MRVSYRRWLLPVVVVLLAPLASFAQGWQHIGSVQKVDKLADGVE
                     LTAGKAKVRVTVFREGIFRVRVAPNGTFPKDFSWAVIEAPQPPAVKVDDSKDEVRVTA
                     GTVVARIRKSPLLIDFLRDSGDVLVADDSNQPMAWNDGRVRVWKRMPEDEGYYGLGDK
                     AGPMNRRNRAFTMWNTDAFGWQESTDPLYKTIPFFMGLRKGIAYGIFFDNTWRGGFDF
                     GKEGHDFYSFGAEGGEINYYFFVGPTPKRIVQDFTALVGRTKLPPLWTLGFQQSRYSY
                     EPESRVYEIAQTFRGKKIPVDALYLDIDYQNANTPFTVDHNKFPTFEKMISDLTAQGI
                     HTVLITDLHIKKDPDHGYAPYDTGVKEDVFVKRADGTTYVGPVWPGDSVFPDFTLARA
                     RDWWGGLYKKFTDMGVSGFWNDMNEPAVFLTINKTMPLDVRHRLDDGTALPHLAIHNV
                     FAMENVRATSEGLLKLRPNERPFVLTRAAYAGTQRYAATWTGDNVSTWNHLGMSTPML
                     LSMGISGYGLVGDDIGGFAGSPEPNLLTRWIEAGVFNPIYRDHTAKGTADQEPWVHGA
                     KHEAIRKRYIEERYRLLPYIYTSVEEMTRTGIPLMRPLLLEYPQSPEFFDDDREYLFG
                     PDLFVSPVTTELQ"
BASE COUNT          774 a         1130 c         1126 g          870 t
ORIGIN      
        1 ttgatcttac tgctgctcgg ctgggtgttc gtgccgtttt acttgcgctc gaacgtcttc
       61 accatgccgc aatttctgga gcggcgtttc agccggcaat gcgcggtgta cctggccggc
      121 atttcgatca tcgcgtacat cttcacgaaa atttcagtgc agttgtatgc ggcgagcgtg
      181 gtcttggagc gcgtagccgg ctggtcgctg tggaaaacgg ccgtggtgct cgtaatcgcg
      241 acgggcattt acaccatcgc gggcggattg gcggcggtga tttataccga cacggtgcag
      301 acgctgatcc tgattacggg cgcggtggcg ctgaccttga tcggactgga caaggtcggc
      361 ggcatcgggc acctgcgtac catggtgccg gacagctatt tccacatgat gaagccgatg
      421 agcgatccgc agtttccgtg gacaggaatt ttcttcggcg cgccgattct tggcatctgg
      481 tattggtgca ccgaccaggt gatcgtgcag cgcgtgctgt cggcacgcga cgaagggcat
      541 gccaaagccg gtacaatttt cgccggcttc ctgaaaattc tgccggtgtt catgctggtg
      601 ttgccgggga tcatcgccta cgcgctgtat ccggcggaag ttgccaagcc ggacttcgcg
      661 tatcccacgc tggtgttgaa tcttctgccg gtgggtttgg tgggattggt gatggccgcg
      721 ttgcttgcag ccgtgatggg agcgatgagc tcagtgttca actccgcttc gacgctggta
      781 acactggatt tttacaagaa gctgcggccc caagcgtcgg aagcgcagtt ggtcaacttc
      841 ggccgcatcg ccaccggcgg aatggttttg ctaggcctgc tgtgggtccc gttcattcac
      901 ctgctcagcg cgcaactgta tatttacttg cagagcgtgc aggcctacat cagcccaccg
      961 atcgctgtat gtttcatttt tggcattttg tggacgcgcg tgaacggcca aggcgccatc
     1021 agctcgctgt tgaccggttt cgtgctcggc gcactgcgtt tcgtgttcga agtgctcgac
     1081 aaaacccgcc actacgaatc ggcctttgtg cggtggctgg tggacatgaa ctttctccac
     1141 tacgccattt ttatgtttgt cgtatgcacc gcggtcctca ttggcgttag cctgatgtat
     1201 ccggcgccgg accgcaaaaa gcttgccggc cttacctttg ccacggtgga cgacaaaatc
     1261 gactccgcgg aagtcgccag accacatctg gccaaggaaa cgtccacaga gcacgcaatt
     1321 aacgtcgcgc ttaccctgct gctgctggcg acagtcgttg gcttgtggat ttatttccgc
     1381 taggagcgct cgaaacagca tggaaatgaa atccattcgc agcttgcggc ccaacgtcac
     1441 gcgtgaggaa gccatccgca tgttttccgc gcccgggccg gcgtcgtttc tttggaagct
     1501 gcgcagcggc ccgttgcagc gtatcgccga cgcctacatt cccttcaatt tgtatcgcgt
     1561 ggcctatcac atgggcggcg cgccgcgctc tcatttgttt gcgctggatg cggtggacgg
     1621 ttcgctcgat ctcttccaat tcggcgcggc gccaaatgac gcggagacgg tgcacgtttc
     1681 gacgcggaat catccagccg cgaaactctc atcctcgaaa gctgaagaat tgttgcgcga
     1741 gaaagtgctt cggctgattt tccaacaggg tttcttcaag gtacgcgcgg tgaaactgga
     1801 aatcgagcgc ctgcccggag aaatccatct gccttactgg ctcggatttt acggaacgca
     1861 ggaaaacctg cgttgccgcg tgatggacgc ggttcgccgc cgagtggaag gcgcgcgagc
     1921 ttccgcacta ttcgaacagt ggctcgccgc ctgaactgtc ccacgagccg aaatttccgg
     1981 aggaacccat gcgagtctct tatcgacgct ggttgctgcc tgttgtagtg gtactgctcg
     2041 cgccgctagc ctctttcgcg caaggctggc agcacatcgg cagcgtgcaa aaagtcgata
     2101 agctcgccga cggagtggaa ctcacggccg gcaaggccaa agttcgcgtg acagtgtttc
     2161 gcgaagggat ttttcgcgtg cgcgttgcgc ccaacggcac tttccccaag gatttttcct
     2221 gggcggtcat cgaagcgcca cagcctccgg cggtaaaagt cgacgattca aaagatgaag
     2281 tgcgagtcac cgccggcacc gtggtggctc gcattcgcaa atcgccattg ctaattgatt
     2341 ttctgcgcga cagcggcgac gtgctggtgg ccgacgattc gaatcagccc atggcgtgga
     2401 acgacggccg cgtccgtgtc tggaagcgca tgcccgaaga cgaaggctac tacggcctgg
     2461 gcgacaaagc cggtcccatg aatcgtcgca atcgcgcctt cacaatgtgg aacaccgacg
     2521 cattcggctg gcaggaatcc accgatccac tttacaagac cattccgttc ttcatgggcc
     2581 tgcgcaaagg cattgcctac ggcatttttt tcgataacac ctggcgcggc ggcttcgatt
     2641 tcggcaaaga gggtcacgac ttctattcgt tcggagccga gggcggcgaa atcaactact
     2701 actttttcgt gggtcccacc ccgaaaagaa tcgtccagga tttcacggca ctggttggcc
     2761 gcaccaagtt gccgccgctg tggacgctgg gcttccagca atcgcgctac agctacgagc
     2821 cggaatcgcg cgtctatgaa atcgcccaaa cgtttcgcgg caaaaagatt cccgtcgacg
     2881 ccctctatct cgacatcgat tatcagaacg cgaacacgcc ctttaccgta gaccacaaca
     2941 aattccccac tttcgaaaaa atgatcagcg atctgacggc gcaggggatt cacacggtgc
     3001 tgatcaccga tttgcacatc aagaaagatc ccgaccacgg ttacgctcct tatgacaccg
     3061 gcgtgaagga agatgtgttc gtcaagcgcg ccgatggcac cacgtatgtg ggtcccgtgt
     3121 ggccgggtga cagtgttttc cctgatttca cgctcgcgcg cgcacgcgac tggtggggcg
     3181 gcctgtacaa aaagttcacc gatatgggcg tctctggatt ttggaacgat atgaacgagc
     3241 cggcggtttt cttgacgatc aacaagacca tgccgcttga cgtgcgtcat cgcctcgacg
     3301 acggcacagc acttccgcat ctcgcgatcc acaacgtttt tgcaatggaa aatgtccgcg
     3361 ccaccagcga aggtttattg aagctgcgtc ccaatgagag gccgtttgtt ttgacgcgcg
     3421 ccgcttacgc gggtacgcaa cgctacgcgg ccacgtggac cggtgacaat gtcagcacct
     3481 ggaaccatct aggaatgagc acgccgatgc tgttgagtat gggaatttcc ggctacggct
     3541 tggtgggcga cgacatcggc ggcttcgccg gttcgcccga gccaaacctc ctcacgcgtt
     3601 ggatcgaagc cggagtcttc aatccaatct accgcgatca caccgcaaaa ggcaccgcgg
     3661 atcaggagcc gtgggtgcac ggcgcaaaac acgaagccat tcgcaagcgc tacatcgagg
     3721 agcgctaccg cttgctccct tacatctaca ccagcgtcga ggaaatgacg cgcaccggaa
     3781 ttccgctgat gcgtccgctg ctgctcgaat atccgcaatc gccggaattc tttgacgacg
     3841 atcgcgaata ccttttcggg cccgacctgt tcgtttcgcc ggtcaccact gaattacagg
//
LOCUS       DASTPM010000348         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_502315, whole genome shotgun sequence.
ACCESSION   DASTPM010000348 DASTPM010000000
VERSION     DASTPM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502315"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VFR42_06070"
     CDS             <1..1497
                     /locus_tag="VFR42_06070"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEU0368753.1"
                     /translation="RVECARLVRQVDAKMRTGYSNATARELLGALEQEFAPEIQVAAF
                     AELRVEDAYVRAGGIGGQPLADDYHFAKTVVDDFGRPFGNGGNAVAGVSIRSSLGPVA
                     AYVQGEYQHAGTLAAQSPAELQAIATQDEAPFAVPRRTGSLDRFRFLDSYGSINFHNN
                     VISFGKQTLWWGPGGDAPFLFSNNAEPLPMLRISRATPFKLPWLFRLMGPIRFELMWG
                     RLEGQQFVSLIDAAGTRSVVGPVLNPHPFVDGEKFSFKPTQNLEFGFGVTTVFSGPGF
                     PFTLKQILRTYKLSNTIPGEAGDPGDRRSAFDFSYRLPGLRNWATLYTDSFTEDEFSP
                     ISFPRKSSFRAGIYMPQLPKLPRADFRVEGIYTDIPSLRGTGVAYFNTHYLSGYTNFG
                     QIIGSWIGREGSGVSAWGTYHFSPQNAVQLRFRDQHVNPSFLGGGHMRDYSVNGTLAK
                     IGGLVLSGNLQYEHWAFPVVAAGAKSNVMANIEVEFRPTGGWLLWSKK"
     gene            1918..2445
                     /locus_tag="VFR42_06075"
     CDS             1918..2445
                     /locus_tag="VFR42_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368754.1"
                     /translation="MKDFESSIEFPKPTTPGDLGAASLGLAFGYFGDVLLTHYFPHLG
                     IPPGEASIYTSATVLGAKNIVQSIVKRRKDKLEAERSSPLSRAKKLDEAMAQEVAKKL
                     TDKAQRPESRAKEMVGEYRDSLLETIFLYKEGLITDDMLSAKMSKCISELTRARQLRE
                     DANMREAHSDETSPL"
BASE COUNT          578 a          640 c          748 g          542 t
ORIGIN      
        1 cgcgttgaat gcgcgcggct ggtccgccag gtagatgcca agatgcgcac cggatactcc
       61 aatgccacag ccagagagtt gctgggggca ttggagcagg agtttgcgcc ggaaatccag
      121 gttgccgctt ttgcagaatt aagagttgag gatgcgtatg tccgggccgg gggcattggt
      181 ggccagccgc tggcggatga ttatcatttc gctaaaaccg tggttgatga ctttggccgg
      241 ccttttggca acggcggcaa cgcggttgcg ggagtctcca tccgttccag cctggggccg
      301 gtagccgcgt acgttcaggg cgaataccag catgccggga ccctggcagc gcagagtccg
      361 gctgaattgc aggccatcgc cacgcaggat gaagcgccgt ttgcagtacc tcggcgcacc
      421 ggatcgctcg accgattccg ctttcttgac agctacggct ccatcaattt ccacaacaac
      481 gtaatctcgt tcgggaagca gacattatgg tggggaccgg gcggcgatgc gccgttcctg
      541 ttcagcaaca atgctgagcc gctaccgatg ctgcggattt cgcgagcgac gcccttcaaa
      601 ctgccgtggc tgttccgcct gatgggccca atccgttttg aactgatgtg gggacggctg
      661 gaagggcagc agtttgtgtc actgattgac gcggcgggaa cgcgaagcgt ggtggggccg
      721 gtattgaacc cgcatccttt tgtcgatgga gaaaaattct cattcaagcc cacgcaaaac
      781 ctggaattcg ggtttggcgt aacgacggtg ttcagcgggc cgggcttccc gtttacattg
      841 aagcagattt tgcggaccta caagctcagc aacacgattc cgggagaggc cggagatccc
      901 ggcgaccggc gctcagcgtt cgacttctcg tatcgcctgc cgggactgcg caactgggcg
      961 acgctgtaca cagactcgtt cactgaggac gaattttctc cgatatcgtt tccgcggaag
     1021 tcgtcattcc gcgcggggat ttacatgccg caactgccga agcttccgcg ggcggatttc
     1081 cgggtggaag gtatttacac cgatattccg agcctgcgcg gtaccggagt ggcgtatttc
     1141 aacacgcatt acctcagcgg ctataccaac tttgggcaga taatcggcag ctggattgga
     1201 cgggagggaa gcggcgtcag cgcctggggc acctaccatt tttcaccgca gaacgccgtt
     1261 cagctgcgtt tccgcgatca gcacgtgaac ccgagctttc ttggaggcgg gcatatgcgc
     1321 gactacagcg tgaatggaac cctggcgaag atcggcggcc tggtgttgag cggcaacctg
     1381 caatacgagc attgggcatt tccagttgtg gcggccggcg cgaaatccaa cgtgatggcg
     1441 aatatcgaag tagagtttcg acccacgggc ggctggctgc tatggagcaa gaagtaaggg
     1501 cggcctcgct cacgcgcgaa aagaggggct gacccggttg cccgcgcact ataaccgaac
     1561 ctaagcggcg agaaacgcag gaaggacacg tatgggagag tgttagctta gacgcggggc
     1621 agatcgcctt ttaaggaaaa gagaaacttg tcatgaagag aaagtccgca agcctgttcg
     1681 caatctcgtt cgttgccgga attgttggct attcgctgaa ggacattgtg cacaacttgg
     1741 ttccaggaag tgcatggttt ttaggaagct tcgctctcgt attcacactt tcatatcttt
     1801 atttcgcgtg ggctgcaagc caagccaata ttcgcgcaga gctacgtcac cttcgagaat
     1861 tgggggagtc tggacgagaa gctgtgaccg acggtagaaa ataagaaagc agatatcatg
     1921 aaagactttg aatcttccat tgagtttcca aaacctacta ctccaggcga cttaggagca
     1981 gcaagtctgg ggctggcatt cggatatttt ggtgacgtcc tgcttaccca ctactttcca
     2041 cacctcggaa ttccacccgg agaagcaagt atctacacgt cagcgacggt gctcggcgca
     2101 aaaaatattg ttcaatcgat cgtgaagcgt cgaaaggata aattggaagc tgaacggagc
     2161 agccctttgt cgagagccaa aaagctcgat gaggccatgg cgcaggaggt ggctaagaaa
     2221 cttaccgaca aagcgcagcg tccggaatcc cgagctaagg agatggtcgg agagtatcgt
     2281 gattctctcc tggagacaat ttttctctac aaagaaggac tgattacgga tgacatgtta
     2341 agcgccaaaa tgtcgaagtg tatttcagag ctgacacgcg cccgacagct tcgtgaagac
     2401 gcaaatatgc gtgaagctca tagtgatgag accagtccgt tgtgagggtt cttctatgtt
     2461 ccccacaagc attccaaaac aaaattcgcg ggttgcagtt agcgacaa
//
LOCUS       DASTPM010000349         3595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_733002, whole genome shotgun sequence.
ACCESSION   DASTPM010000349 DASTPM010000000
VERSION     DASTPM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733002"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFR42_06080"
     CDS             <1..461
                     /locus_tag="VFR42_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0368755.1"
                     /translation="SNFATNLIIEKLGVENIRATVHALGADGVVVLRGVEDGKAFEKG
                     LNNTATARGLAILLEAIAQEKAVDAASSQEMIAILKRQKFNEGIPAGLPLGISVAHKT
                     GEITKIHHDAAIVFAKRPYVLVILVRGLADTKQSAALMADISRQIYSATQ"
     gene            576..1421
                     /locus_tag="VFR42_06085"
     CDS             576..1421
                     /locus_tag="VFR42_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368756.1"
                     /translation="MKTLSRALLAGGSLLLLAGIGFADTLELKDGRVLQGKYLGGTQT
                     VLRFQVNGDVQTFPTNDIVALTFTRNSGSAPAPAAAPPPQEEPAPAPVPSQAAPPPAA
                     ANSYAGGDVSVPAGQSILVRMIDGVDSSKNHVGDLFHASLETDLNVNGVLVARKGTDV
                     YGRLASSDKGGTFSGKSELQLELTRLVIDGRDYPVVSSDYNVQGKSQGSSTAKKVGGG
                     AVAGAIIGAIAGGGKGAAIGAAAGGGAGAGVQILTKGDRVKVPSETLLEFRLQQPVMI
                     TPTAR"
     gene            1565..1981
                     /locus_tag="VFR42_06090"
     CDS             1565..1981
                     /locus_tag="VFR42_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0368757.1"
                     /translation="MPKEKPQVNIGEVIKTYRSQRGLSQGDIERRTGLLRCYLSRVEN
                     GHTVPSLETLAKIAEAMDISLADFFPGTETPRDKETQKMLGELSQDEIRFLVEIKRYS
                     STLSDGDKRLVLAMIRKMATMIPPPVRKPLVRRPAV"
     gene            complement(2044..3039)
                     /locus_tag="VFR42_06095"
     CDS             complement(2044..3039)
                     /locus_tag="VFR42_06095"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0368758.1"
                     /translation="MPATRQLSAAPLLALALLLTIVLAIFSSTSGNGYWHADRSFLAT
                     LVVFASFLSIMLLFAARGIAERFSTLAGPGGSWLLTLMLFFLFLVYALGTGTASLWRI
                     ATAAAFLFAPMLLLASAQGAAPGTWQDFATIAVIWTAVKFGPSHWLWPYPGSRLAYIF
                     TVITAVNVAIAGFLLLRRTKNVGYNIGWGSGWRLYIFGSLAMFACIAIPVGLQMHFIA
                     YAPRFSEWKTFIPLSIGILFFTAWPEEFLFRGLLQNLSSRASNSETVGWIAASVLFGF
                     SHITNLHFPNWKYVLLASVAGFFYGWTWRKTGSIFASALVHAGVDILWHFLFHTI"
     gene            3082..>3595
                     /locus_tag="VFR42_06100"
     CDS             3082..>3595
                     /locus_tag="VFR42_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEU0368759.1"
                     /translation="MTRRLTYIDWMRGLACVVMFQTHCYDSWLNPQARQTTFFAWSRL
                     GGTLPAPLFIFLAGLSVALVTEKLREKGIARNEIARRTIFRGVEVFGLGILFRIQEFA
                     LGYPRSPWTDLFRVDILNILGLSMMLMAAVCWLAGSGSPAAARNRSLAAGIAAAALVA
                     LATPGLWTIWR"
BASE COUNT          802 a         1041 c         1058 g          694 t
ORIGIN      
        1 gcagcaattt cgccacaaat ctcatcatcg agaagctggg agtggagaac attcgagcca
       61 ccgttcacgc gctcggtgcg gacggcgttg tcgtcctgcg tggcgtggag gacggcaagg
      121 cattcgaaaa gggcctgaac aataccgcca ccgcccgcgg gcttgcgatc ctcctcgaag
      181 ctatcgcaca agaaaaggcc gtggacgcag ccagctcaca agaaatgatc gctatcctca
      241 agcggcagaa gttcaacgaa ggaatcccgg cgggactgcc tttgggcatt tctgtcgccc
      301 ataagaccgg cgaaatcacc aagattcatc acgatgcggc catcgtgttc gccaagcgtc
      361 cctacgtgct ggtgattctg gttcgcggcc tggcggacac caaacaaagc gcggcgctca
      421 tggcggacat cagccgccag atttacagtg cgacgcagta ggtcaccgtt agcgcaaccc
      481 aggagagcag tgcgaacatc caagtgttga ggatttgtgt aaatatctag gacaggacat
      541 ctccccggat accgggacag agaagggact tcatcatgaa gactttgtcg agggccttgc
      601 tggcaggtgg cagcctacta ttgctcgcgg gaatcggttt tgccgacacg ctggagctca
      661 aggatggccg cgtgttgcag ggcaagtatc tgggcggcac gcagaccgtc ttgcgcttcc
      721 aggtaaacgg cgacgtgcag acgtttccta ccaatgacat tgttgcgctg accttcacgc
      781 gcaattctgg ttcggcgcct gctcctgctg ccgctcctcc gccgcaggag gagccagcac
      841 cggctccggt gccgtctcag gctgctccgc cccctgctgc tgccaactcc tatgcgggtg
      901 gtgacgtcag tgtacccgcg gggcaatcga ttttggtccg catgattgat ggcgttgact
      961 cttccaaaaa ccacgtcggc gatcttttcc acgccagtct cgaaaccgac ctaaacgtga
     1021 acggcgtctt ggtcgcgcgc aagggaactg acgtctacgg acgcttggca agctcggaca
     1081 agggcggaac tttctccggg aagtccgagc tgcaactaga gctgactcgg ctggtcatcg
     1141 atggcaggga ctatcccgtg gtcagcagcg attacaacgt gcagggcaag agccaaggct
     1201 cgtcgaccgc gaagaaggtc ggcggcggtg ccgtcgcagg agccatcatt ggcgccatcg
     1261 ctggcggtgg caagggagcc gcaatcggcg cggcggccgg cggtggtgct ggtgccgggg
     1321 tacagatcct gaccaagggt gatcgtgtga aggtgcccag cgaaaccctg cttgaattcc
     1381 ggctgcagca gccggtcatg attacgccga ctgcgcgcta gattcgcgcc gataagtcct
     1441 ccgataaatc ccgtctgacc agttacttgc cgggcagggg tgtctcttag gaatcattac
     1501 cggcaaattc cggcttgccg attctgctgt tagtgactat gctaacacct caagtggggg
     1561 agccatgccg aaagagaagc cgcaagtaaa catcggggaa gtcatcaaga cctatcgcag
     1621 ccagcgcggg ctgtcgcagg gcgacatcga acgccgcacc ggactgctgc gctgctatct
     1681 gtctcgcgtg gaaaacggcc acaccgtgcc gtcgctcgag acgctcgcca aaatcgccga
     1741 agccatggac atttccctgg ccgatttctt tccgggcacc gaaacgccgc gcgacaaaga
     1801 aacccaaaaa atgctcggcg agctttcgca ggacgaaatc cgcttcctgg tcgaaatcaa
     1861 acgctacagc tccacgcttt ctgacggcga caagcgcctg gtgctggcca tgatccgtaa
     1921 aatggccacc atgattcctc caccggtacg caagccgctg gttcggcgtc ctgcggtctg
     1981 agcggccggc tcgacgcttt tgcgggctga tctgcacttg cggatcagcc tattttgttg
     2041 aggtcagatg gtgtgaaaca gaaagtgcca cagaatgtct acaccggcgt gcaccagcgc
     2101 cgaggcaaaa atcgatccgg tcttccgcca ggtccaaccg tagaaaaatc cggcgacgga
     2161 tgccagcagg acgtacttcc aattcggaaa atgcaggttc gtgatgtgcg aaaagccgaa
     2221 gagaacggaa gccgcaatcc agcccacggt ttcgctgttg ctcgctcgcg atgacagatt
     2281 ctgaagcagc ccgcgaaaca aaaattcctc tggccaggct gtaaagaaga gaatgccgat
     2341 cgagagaggg atgaaagtct tccattcgct gaagcgcggc gcgtaggcga tgaagtgcat
     2401 ttgcaatccc actggaatcg cgatgcaggc aaacatcgcg agcgaaccga agatgtaaag
     2461 cctccagccg ctgccccacc cgatgttgta gcccacattc ttggttcgcc gtaaaagcag
     2521 gaagccagcg atggctacat tcaccgcggt aatgaccgta aaaatgtagg caaggcggct
     2581 gccgggatat ggccacaacc aatgcgaagg cccgaatttc accgctgtcc aaatcactgc
     2641 gatcgttgcg aagtcctgcc aggtgcccgg tgctgctccc tgcgcggatg caagcaacag
     2701 catcggcgca aacaagaacg cagccgcggt ggcaatccgc cagagcgaag cagttccagt
     2761 tccgagcgcg taaacgagaa agagaaaaaa caacatcaag gtcagcagcc atgagccgcc
     2821 agggccagcg agcgtggaga atcgttccgc gatgccacgc gccgcgaaca acagcatgat
     2881 ggaaagaaag ctggcgaaga cgaccagcgt ggcgagaaac gaccggtcgg catgccaata
     2941 gccattgccg cttgtggacg aaaatattgc caggacgatg gtcagcagca gggccagcgc
     3001 aagaagtggc gctgcgctca gttggcgagt agcaggcaag caagtattgt atcgtaacga
     3061 cttttatcga cagctctttc gatgaccagg cgactcactt acatcgattg gatgcgcggc
     3121 ctcgcgtgcg tcgtgatgtt tcaaacgcac tgctacgact cctggctcaa tccgcaggcg
     3181 cgccagacaa ctttctttgc gtggtcgcgg ctcggcggaa cgctacccgc cccgctcttt
     3241 atttttctgg ctggcctttc cgttgcgctg gtcaccgaaa aattgcggga aaagggaatc
     3301 gcgcgcaatg aaattgccag gcgaactatt tttcgcggcg tggaagtgtt tggccttgga
     3361 attttatttc gcattcagga atttgctctc ggctatcccc gttcgccgtg gaccgatctt
     3421 ttccgcgtcg acatcctgaa tatcctcggc ctctcgatga tgttgatggc agcggtttgc
     3481 tggctcgccg gctctggctc acccgcagct gcacgcaaca gaagtcttgc cgccggaatc
     3541 gctgcggccg ctctagtcgc cttagcgacc cccggattgt ggaccatttg gcggc
//
LOCUS       DASTPM010000350         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_329539, whole genome shotgun sequence.
ACCESSION   DASTPM010000350 DASTPM010000000
VERSION     DASTPM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329539"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFR42_06105"
     CDS             <1..982
                     /locus_tag="VFR42_06105"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0368760.1"
                     /translation="EIDRRVAADNFSGTVMVTKNGKTIFSGAYGLADRQKKIKNDLDT
                     QFHLGSMNKMFTAVSTLQLVQAGKLKLSGTVGEYLPDYPNKDLASKVTIHHLLTHTGG
                     TGDFFGPEFDKHRLELRTLQDYVKLYGQRGLEFEPGSKWDYSNYGFLLLGVIVQKVSG
                     QDYYDYVRQHVYGPAGMTSTDSLPEDQIVAKRSVGYTKPGPGESWRPNTDTLPYRGTS
                     AGGGYSTVEDLQRFADAIKNHKLLDAQNTELLTTGKTETGGGAKYAYGFMDQVSGGVR
                     SYGHGGGAPGMNGDLTIYPQSGYVVAVLANMDPPAAQRIATFIGNRLPAK"
     gene            complement(1058..2122)
                     /locus_tag="VFR42_06110"
     CDS             complement(1058..2122)
                     /locus_tag="VFR42_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368761.1"
                     /translation="MAILLAGTAALFGLGTRSASADDNGEDDAHGRDGYAIGLWGDMP
                     YSDVQATVGVPNIIADMNSQVLAFTAHDGDLKAGSGACSDAVYTRALGYFASLKAPAV
                     FTPGDNDWTDCDRVAGVNSLAQLDKERRLFFSTPFTLGQRKLHQEVQSEPLCLGVNGP
                     TACVENRRWTLGGVTYATLNVQGSCNNLCDTAPDPAEFAARNSANIAWLLQTFADAKQ
                     RGSLAVMLIAQADPGFDATDATRAPVRNPHTLAETDGQPDGFVDFLTALRTEVVAFGK
                     PVAYVHGDSHYFRIDKPFLNAQGQRLENFTRVETFGDHQENGINDNHWLKVFVDPKSR
                     EVFSYQTQIVPANRVAVPAP"
     gene            complement(2359..>2875)
                     /locus_tag="VFR42_06115"
     CDS             complement(2359..>2875)
                     /locus_tag="VFR42_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368762.1"
                     /translation="FVLIGTLQLPWRFELSTLSQFESARPFTIATPADILNDGFSGND
                     RAVVNGVMTSLDQFRGTPFCQVDLRVSRNIALGERVILRPFADFFNLFNRQNPGNNYV
                     GDISALPAPVNSLTNATAFCLDGPACTQTAPITSYKQLRVPAGALGDFFGPGTTVGIP
                     FAAQLGIKLIF"
BASE COUNT          644 a          852 c          794 g          585 t
ORIGIN      
        1 tgagattgac cgtcgagttg ccgcggacaa tttctcaggc accgtgatgg tcacaaaaaa
       61 tggcaaaacc attttttcgg gcgcttacgg tttggccgac cgccaaaaga aaatcaaaaa
      121 cgatttggac acgcagttcc acctcggctc gatgaacaaa atgtttacag cggtttctac
      181 gctgcaattg gtgcaggccg gcaagctcaa gctgagcggt accgtcggcg aatatcttcc
      241 cgactatccg aacaaggatt tagcctcaaa ggtcaccatc catcatctgc tgacgcatac
      301 cggcggcacc ggagatttct tcggtccgga attcgacaaa catcgcctgg agcttcgtac
      361 cctgcaggat tacgtgaagc tttacggaca gcgcggcctg gagttcgaac ccggcagcaa
      421 gtgggattac agcaactacg gtttcctcct gctcggcgtc atcgtgcaga aagtgagcgg
      481 gcaggactac tacgactacg tccgccagca tgtctacggc ccagcgggaa tgacctccac
      541 cgactcgctg cccgaagatc aaatcgtcgc gaagcgctcc gtaggctaca cgaaacctgg
      601 tcctggcgaa tcctggcggc ccaacaccga cactctgccg tatcgcggca cctcagccgg
      661 cggcggctat tctacagtgg aggatttgca aagatttgcc gacgcgatca aaaatcacaa
      721 gcttctcgat gcgcaaaaca ccgagctgct caccacgggc aagaccgaaa ccggtggcgg
      781 tgccaagtat gcttacggtt tcatggatca agtttccggt ggagtgcgca gttacggcca
      841 cggcggtggc gcgccgggaa tgaacggcga tctgacgatt tatccgcaga gcggttatgt
      901 ggtcgccgtt ctcgccaata tggacccgcc cgcggcgcag cgaattgcaa ctttcatcgg
      961 caatcgcctc ccggccaagt gattgcgact gacaaaataa gaggcggcac gcagcgcgca
     1021 ccgcctctgt gcaaaacgag gctgcttttt cccgatttca gggcgccgga acggccacgc
     1081 ggttcgccgg cacgatctgc gtctgatagg aaaacacttc gcggctcttc ggatccacaa
     1141 acaccttgag ccagtggttg tcgttgattc cgttttcctg gtggtcgccg aacgtttcca
     1201 cgcgggtgaa attttccaga cgctgtcctt gcgcattcag aaatggcttg tcgatgcgga
     1261 agtagtgcga gtctccgtgc acgtaagcga ccggcttccc aaacgcaacc acctcggtgc
     1321 gcagcgcagt cagaaagtcc acaaacccgt ccggctggcc atccgtttct gccagcgtat
     1381 gcggattccg tacgggcgcg cgagtcgcat ctgtcgcgtc gaacccagga tcggcctgcg
     1441 ctatgagcat tacggcaagc gagccgcgct gttttgcatc cgcgaaggtc tgcagcagcc
     1501 atgcaatatt cgcactgttg cgcgcggcaa attccgctgg atcgggagcc gtatcgcaaa
     1561 gattgttaca cgagccttgc acgttcaatg tcgcataggt cacgccgccc agcgtccagc
     1621 gccgattttc cacgcacgcc gtgggcccgt tgacgcccag gcacaacggc tcggactgca
     1681 cttcctggtg tagcttgcgt tggccgagtg tgaatggcgt gctgaaaaat agtctgcgct
     1741 ccttatccag ctgagccagc gaattcacgc cggccactcg atcacagtcc gtccaatcgt
     1801 tgtctcccgg agtgaatacc gccggcgcct taagcgaagc aaaatagccc agcgcgcggg
     1861 tatacacagc gtccgagcat gcacccgagc cggctttcag gtcgccgtca tgcgccgtaa
     1921 aagccagcac ctgggaattc atgtctgcga ttatattggg aacaccaacc gtcgcttgca
     1981 cgtccgagta cggcatgtca ccccaaagcc cgatcgcgta cccgtcgcgc ccgtgcgcgt
     2041 cgtcttctcc attgtcgtcc gccgaagccg atctcgtgcc aagaccgaag agagctgccg
     2101 tacccgccag taaaattgcc acgccgcaaa tccaaagatg ggttgctcct cggcgctgag
     2161 tcgaactttt tgaaatgaat cgcaaatcag gccaaaacat gattgtgcac tcctgagaat
     2221 gaaggatttt tcggccgtgg gggactggac acggcccgct gagacattac gggccgcgcg
     2281 tcgacacagt cttacgctgt ggtgaatgat tggtttaaat tcagaaagtt gcgcgggttc
     2341 cgcgcagtgt ttctactctt aaaagataag tttgattccc aactgcgcgg caaacggaat
     2401 gcccaccgtc gtgcccggcc cgaaaaaatc tcccaaggcg cccgccggaa cgcgcaactg
     2461 cttgtagctg gtgatcggag cagtttgcgt gcaggctggg ccatccaggc aaaatgcggt
     2521 cgcattcgtc agactgttca ctggtgcagg caatgctgaa atatctccga catagttatt
     2581 cccaggattc tgccggttga acaggttaaa gaagtctgcg aagggccgca ggattacccg
     2641 ttcgcccagg gcaatgttgc ggctcacccg caaatccact tgacaaaatg gtgtgccgcg
     2701 aaactggtcc agcgaagtca tcacgccgtt gaccaccgcc cggtcgttgc ctgaaaaacc
     2761 atcattcaaa atatcggctg gcgtcgcaat ggtgaatggg cgcgcgctct caaactgcga
     2821 aagcgtcgaa agttcgaatc gccacggcaa ctgcagcgtt ccgatcagca caaag
//
LOCUS       DASTPM010000351         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1425274, whole genome shotgun sequence.
ACCESSION   DASTPM010000351 DASTPM010000000
VERSION     DASTPM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425274"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VFR42_06120"
     CDS             <1..464
                     /locus_tag="VFR42_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU0368763.1"
                     /translation="QPNHLMATLKVAETQARDVQIGEPAVVDTHNGTVAGTVMRVDPG
                     VQNGTVTVDVKLTGELPKGARPDLSVDGTIDLERLNDVIYVGRPAFGQENSTISLFRL
                     DPDEKGAVRVPVKVGRASVNSIQVLEGLREHDVVILSDMSRWDNAERIRLD"
     gene            484..1209
                     /locus_tag="VFR42_06125"
     CDS             484..1209
                     /locus_tag="VFR42_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0368764.1"
                     /translation="MNEAEQALIDIEGLTKVFYTDEIETHALSGIHLTIRKGEYVAIS
                     GPSGCGKSTLLSILGLLDSPTAGKYQLNGQPVESLDFAARARIRNREIGFIFQSFNLI
                     GDLTVYENVELPLTYRQKMPSAERKPRVMEALERVGMAHRLRHYPSQLSGGQQQRVAV
                     ARALGGHPSILLADEPTGNLDSHNGEAVMKLLQELHQEGATICMVTHDPRFVLQAERE
                     IRLFDGKVVAEEDLQRQTQDATA"
     gene            1278..>3253
                     /locus_tag="VFR42_06130"
     CDS             1278..>3253
                     /locus_tag="VFR42_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368765.1"
                     /translation="MNGLIHDLRYALRQLRKAPGFSVVAVLTLSLGLSATVAIFAFVD
                     AALLKPLPYRDSSRLVDVTEGSAMFPRANLSYLDYVDWKKMNTVFSSMDVWNGSGYLF
                     QTPTGAVPVSAVRVSDGFFRTLGVKPALGRDFYEGEDKVGAPPTALLSAATWRDRFSS
                     RPDIIGQTVMLSGKAYTIIGVLPKEFQFALRGRAEFWTALQPTMECEKRRSCHNLYGV
                     ARLKDGVTVAAADANLNTVAKQLETQYPDSNRGQFASVITLSDSMVGNVRPLFLMLLT
                     GAGLLFFIACVNVASLLLVRSESRRREMAVRGALGASAGRLFRQFVTEGMVLTAGGIV
                     LGLVLAAWAVGVLIQLIPAEMLGRMPYLQGLGLSSRVILFALGIALVAGVVFAVAPAL
                     RTSFVDLRDDLAEGGRAAAGRVWRKLGANLVTLELATAVILLVSAGLLGKSFFRLLHV
                     DLGFDPGHVASLLVIAPDVGYEKEEQQIALANKTMERFRQLPGVQGVALTSTLPVSCN
                     CNTNWIRFVGKPYSGEHNEVNNRAVTPEFFSTIHAKLLHGRFFTESDDSTKPLVSIIN
                     KTLAQKYFPGEDPIGRKYGDTQLSPKSLREIVGVVDDVRDGSLDAALWPAEYVPFKQN
                     TDSSFYAVVRTSEDERSLLPTLVSAVRRVDPALGT"
BASE COUNT          666 a          880 c          999 g          708 t
ORIGIN      
        1 ttcagccaaa tcatttgatg gcgactttga aggtggcgga aacgcaagcg cgcgacgtac
       61 agatcgggga gccggccgtg gtggacaccc acaatggaac ggtagcgggc acagtgatgc
      121 gggttgaccc gggcgtgcag aacggtaccg tgaccgttga cgtgaagtta accggcgaat
      181 tgcctaaagg cgcccggccc gacttgagcg tagacggaac catagacctt gagcggttga
      241 acgacgtcat ttatgtaggc cgtccagcat ttggccagga gaacagcacg atcagcttgt
      301 tcaggctcga ccccgacgaa aagggggccg tgcgagttcc ggtgaaagtg gggcgtgcgt
      361 cggtgaattc catccaggtg ttggaagggc tgcgggaaca tgacgtcgtc attctttcgg
      421 acatgtcgcg ttgggataac gcggaaagaa tccggttgga ctgataaacg aggggaaaaa
      481 ctcatgaacg aagcagaaca ggcgttgatc gatattgaag ggctcaccaa ggtcttctac
      541 accgacgaaa tcgaaacgca cgcactctcg gggatacacc tgacgattcg caagggcgag
      601 tacgtggcga tctcagggcc gtcggggtgt ggcaagtcca cgttgctgtc gattctgggc
      661 ctgctcgatt cgcctacggc aggaaagtat cagttgaacg ggcagccggt ggagagcctg
      721 gacttcgcgg cgcgagctcg gatccgcaac cgcgaaatcg gatttatttt tcagagtttc
      781 aatctcattg gcgacctcac ggtttacgaa aacgtggagc taccgctgac ctatcggcag
      841 aagatgccgt ctgcggaacg caaaccgcgg gtgatggaag ccctcgaacg ggtcggcatg
      901 gcgcatcggc tgcgtcatta tccttcgcaa ctttccggcg gtcagcaaca acgcgtggcg
      961 gtggcacgcg cgctgggcgg ccatccttcc atcctgctgg cggacgagcc aaccgggaac
     1021 ctcgactccc acaatggcga agccgtgatg aagctcctgc aagagttgca tcaagagggc
     1081 gcgacgatct gcatggtgac gcatgaccct cgctttgtgc tgcaggccga gcgcgaaatt
     1141 cgtcttttcg acggcaaggt tgtggccgaa gaagacctgc aaaggcaaac gcaagacgca
     1201 accgcataaa gcgaaggttt tcaggtcatt cgctaggatg ttggggcggc gttacgagcg
     1261 ggcggggtga acgtgcaatg aacggactaa tccacgatct gcgatatgca ctgaggcagt
     1321 tgcggaaagc gcccggtttc agcgtggtcg ccgttcttac cttgtcgctg ggccttagcg
     1381 ccaccgtggc aatttttgct tttgtcgacg ccgctctact aaagcctttg ccttatcgcg
     1441 attcctcgcg gctggtggac gttaccgaag gcagcgcgat gtttccgcgc gccaatcttt
     1501 cttatctcga ctacgtcgac tggaaaaaga tgaacacggt tttcagttca atggacgtct
     1561 ggaacggcag cggctatttg tttcagacgc cgaccggcgc cgtcccggtg tcggcggtgc
     1621 gagtaagcga cggctttttc cgcacgcttg gcgtaaaacc cgccttgggc agagatttct
     1681 atgaaggtga agataaggta ggtgcgccgc caactgctct gctaagcgct gcgacttggc
     1741 gggatcgctt ctccagtcgc cccgatatta tcggccaaac cgtaatgctc agtggcaagg
     1801 cctacacgat cattggcgtc ctgccaaaag aattccagtt tgcgttacgt ggccgggcgg
     1861 aattctggac tgcgctacag cccacgatgg aatgcgaaaa gcggcgtagc tgccacaact
     1921 tgtacggggt ggcacgttta aaggatggag tcacagttgc cgccgccgac gcgaacctga
     1981 ataccgtcgc gaagcagctg gaaacacaat atccggattc caatcgcggc caatttgcct
     2041 cagtcatcac gcttagcgac tcgatggtcg ggaatgtcag gccgctcttt ctgatgctgc
     2101 tgaccggcgc cggacttctc ttttttatcg cgtgcgtcaa tgtggccagc ctgctgctgg
     2161 ttcgttcgga aagccgccgg cgagaaatgg cagtacgcgg cgctttgggt gcttcggctg
     2221 gccggctttt ccgtcaattc gtgaccgaag gcatggtcct tacagccggt ggcattgtgt
     2281 tgggactcgt tcttgccgcc tgggccgtgg gtgttcttat tcaactgatc cccgcagaaa
     2341 tgctcggccg catgccgtac ttgcagggac tcgggctaag ttcccgggtg attttgttcg
     2401 ccctgggcat tgcactggtc gctggcgtgg tgttcgccgt ggcaccggcg ctgcgcacat
     2461 cattcgtgga tttgagagac gacctggctg aaggaggacg cgccgccgcg ggccgcgtct
     2521 ggagaaaact aggcgccaac ctcgtgacgc tcgaattggc aacggcggtc atcttgttgg
     2581 ttagtgccgg gctgcttggt aagagtttct ttcgtttgtt gcatgtagac ctgggcttcg
     2641 atcccgggca cgtcgcatcg ttgctggtca ttgctccgga tgtcggttat gagaaggaag
     2701 aacagcaaat cgcgctcgcc aacaagacca tggagcggtt tcgccaattg cccggcgttc
     2761 aaggagtggc ccttaccagt acgctgcctg tgagctgcaa ctgcaacacc aactggatcc
     2821 gctttgttgg gaagccttac agtggcgagc acaatgaagt gaataatcgc gcggtcacgc
     2881 cggaattctt ttccaccatt cacgccaagc ttttgcacgg ccggttcttc acggagtcag
     2941 atgattccac caagccgctg gtctcgatca ttaacaagac gctggcccag aagtattttc
     3001 ccggcgaaga ccccattgga cggaaatatg gggacaccca actgtcgccg aaatctctgc
     3061 gcgaaatcgt cggagtcgtg gacgacgtgc gcgatggttc gctggacgcc gctctctggc
     3121 ccgccgagta tgttccgttt aaacagaata ccgacagttc gttctatgcc gtggtccgta
     3181 catccgagga cgagcgttct ttgttgccga cgctggtctc ggccgtacgt cgggtagatc
     3241 cggcccttgg cac
//
LOCUS       DASTPM010000352         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1108162, whole genome shotgun sequence.
ACCESSION   DASTPM010000352 DASTPM010000000
VERSION     DASTPM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108162"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..2109
                     /gene="thrS"
                     /locus_tag="VFR42_06135"
     CDS             124..2109
                     /gene="thrS"
                     /locus_tag="VFR42_06135"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0368766.1"
                     /translation="MDTIHITLPDGSVKDVPKGTTAADIARSISPRLADAAFVAKAYS
                     NNGPLQNFGEPVDDGGYLIDLRRPLEQDLRLKILTDRDSDALFVFRHSAAHLLAAAVM
                     ELYPDVKLGIGPPVDNGFFYEFLREEPFTPDDLAKIEKKMHELAAQDLPNERKYMPKP
                     EALEFYKKSNQIFKCELVEEKVTEPMVSFYTTGKFIDFCRGPHIPSTKRIQAFKLMNV
                     AGAYWKGQEGNAQLQRIYAAAFFTQKELDEYLHRLEEAKRRDHRKLGPELDLFSIQEE
                     AGPGLIFWHPKGGFIRNIIENWLRDELIKRGYDLVYTPHIMRLDLWKTSGHAGFYREN
                     MFGPIEVEKADYQLKPMNCPGHILIYKSKLRSYRDLPVRLAELGTVYRYERSGVLHGL
                     LRVRGFTQDDAHIFCMPSQIEAEVQACIDFAYAVMKNFGFEKFEVELSVWDSKHPENY
                     AGKAEDWERAAAALANTMDRLKIPYKKIEGEAAFYGPKIDVKLIDAIGRSWQLTTVQF
                     DFNLPARFGLEYVGDDGARHQPLMVHRALLGSVERFFGILIEHYAGAFPLWLAPVQVE
                     ICPVSEKVMDYAKHVQETLKNHGIRVHLDDRNEKLPAKIRDAQLQKIPYMLVVGPKEA
                     EAGTVSVRHRGKGDLGPKPLADLVESLRSESDSRAIG"
BASE COUNT          478 a          711 c          516 g          423 t
ORIGIN      
        1 cactacgctg tgcggttgtt tgtcggttga ctacgcctcg agcgcccgtc gcggcttgac
       61 ccccttttgc gctgccgata ctctacttct ttcccttcag caaacgggag cccgttctca
      121 cacatggata ccattcacat cactttgccg gacggcagcg tcaaggacgt ccccaaaggc
      181 accactgccg ccgacattgc gcgcagcatt tcgccgcggc tcgccgacgc cgccttcgtc
      241 gcgaaggcct attccaacaa cggccctctg cagaattttg gcgaaccagt ggacgacggc
      301 ggttacttga tcgatttgcg gcggccgctg gaacaagacc tcagactcaa aatccttacc
      361 gacagagact ccgacgcgct tttcgtcttt cgccactccg ccgcgcatct tctcgctgcc
      421 gccgtcatgg agctctatcc cgacgtcaag ctcggcattg gcccgccggt ggacaacggc
      481 ttcttctacg aatttctgcg cgaagagcct ttcactcccg acgacctggc caaaatcgaa
      541 aagaaaatgc acgaactcgc ggcacaggac ttgcccaacg agcgcaagta catgcccaag
      601 cccgaagcgc tggagttcta caaaaagtcc aaccagatat ttaagtgtga gctggtcgaa
      661 gaaaaagtca ccgagcctat ggtctccttc tacaccacgg gcaagttcat cgacttctgc
      721 cgcggccccc acatcccctc caccaagcgc attcaggcgt tcaaactaat gaacgtcgcc
      781 ggcgcttatt ggaaaggcca ggaaggcaac gcccagctgc agcgcatcta cgccgccgcg
      841 ttcttcacgc aaaaagaact cgatgaatat ctccaccgcc tggaagaagc caagcgccgc
      901 gaccatcgca agctcggccc cgagctcgat ctcttcagca ttcaggaaga agccggcccc
      961 ggcctgatct tctggcatcc caagggcggc ttcatccgta acattattga aaactggttg
     1021 cgcgacgaac tcatcaagcg cggctacgat ctcgtctaca cgccgcatat tatgcgtctc
     1081 gatctgtgga agacctccgg ccacgccggc ttttatcgcg aaaacatgtt tggacccatc
     1141 gaagtcgaaa aagccgatta tcagctcaag cccatgaact gcccgggcca catcctcatc
     1201 tacaaatcca agctgcgcag ctatcgcgat cttcccgtcc gcctcgctga actcggcact
     1261 gtatatcgct atgagcgcag cggtgtcttg catggtctcc tgcgtgttcg cggcttcacc
     1321 caggacgacg cgcacatctt ctgcatgcct tcgcaaatcg aggccgaagt ccaagcctgc
     1381 atcgacttcg cctatgccgt catgaaaaat ttcggctttg aaaaatttga agtcgaactt
     1441 tccgtctggg attcgaagca cccggaaaat tacgccggca aagccgaaga ttgggaacgc
     1501 gccgccgcgg cccttgccaa caccatggac cgcttgaaaa tcccttacaa aaagatcgaa
     1561 ggcgaagccg cattctacgg ccctaaaatc gacgtcaagc taatcgacgc catcggccgc
     1621 tcctggcagc tcactaccgt tcagtttgat ttcaatctgc ctgcgcgctt tggcctcgaa
     1681 tatgtcgggg acgatggcgc tcgccatcag cctttgatgg tgcaccgcgc gctgctcggc
     1741 tctgtcgagc gcttcttcgg tatcctcatt gagcactacg ccggagcgtt tccgctatgg
     1801 ctcgcgcccg tgcaagtgga aatctgtcca gtcagcgaaa aagtcatgga ctacgccaag
     1861 cacgtccagg aaaccctgaa gaaccacggc atccgcgtcc atctcgacga ccgcaacgaa
     1921 aagctgcccg ccaaaatccg cgacgcacag cttcagaaaa ttccctacat gctcgtcgtc
     1981 ggccccaaag aagccgaagc tggcaccgtc agcgttcgcc accgcggcaa aggcgacctc
     2041 ggccccaagc ccttagccga tctggtcgaa tccctgcgct ccgaatccga ctcccgcgcc
     2101 atcggttagc gcaagccatc accaccca
//
LOCUS       DASTPM010000353         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_416268, whole genome shotgun sequence.
ACCESSION   DASTPM010000353 DASTPM010000000
VERSION     DASTPM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416268"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..857)
                     /locus_tag="VFR42_06140"
     CDS             complement(438..857)
                     /locus_tag="VFR42_06140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0368767.1"
                     /translation="MVLSNRKEIRVEWGDCDPAGIVYFPRFFEYFDACTNALFEAAGF
                     RKAEMLKYYGLVGIPLVEASAQFYAPASFGDTVVVESRIVEWGKSSFRVEHALYKGNV
                     LGAEGREKRVWTVRDASSEKGMRGEAIPEEVKARFAK"
     gene            950..2323
                     /locus_tag="VFR42_06145"
     CDS             950..2323
                     /locus_tag="VFR42_06145"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEU0368768.1"
                     /translation="MQLLDGFFRSPTVEPFFTDAAAVQSILDFEAALARAQARAGLIS
                     ENFASTIASFCRAELVDLPALAQAMPSAGNLAIPLVKQLTASVARTSPDAACHVHYGA
                     TSQDALDTGLVLQLRSATRALHADLETIISSLAELTSAHRKTLLVARTWLQHALPTTF
                     GFITAGWLDACLRHRERLNALLEHSLVLQFGGAAGTLAALRDRGALVASLLAEELALP
                     LPRIPWHAQRERIAEVATTYGLVSGTFAKIARDLSLHMQTEVAELGEPTATGRGGSST
                     MPHKQNPVACAAILASTSRVPPLVSTILFGLSGEYHRSLGPWQSEWETLPEIVRLSAG
                     ASHHLAGLLPRLTIHSDRMRANLELTHGLIYAEAITLALSGTLNRASAHKLVEEACRR
                     AQSERRHLREILSADAEVNAILDAQSIAQLFEPANYLGSAGAAIETVLSAAKSQPRSA
                     TKSNATG"
     gene            2327..3106
                     /gene="pcaD"
                     /locus_tag="VFR42_06150"
     CDS             2327..3106
                     /gene="pcaD"
                     /locus_tag="VFR42_06150"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEU0368769.1"
                     /translation="MPFANLPNVRLHYALSGLPDAPVLILSNSLGTNFSMWDGQAPAF
                     EKQFRLLRYDMRGHGQSSAPPPPYAVPDLACDILSLADSLSIPRFHFCGLSIGGMIGM
                     SLAFQAPERLHKLVLCNTAPKIGALEFWNTRIQNVQSKGMKEVAGAVTARWFTPGFQQ
                     SSPEKVASAIRVLESLDPQGYAGGCTAVRDFDFRESVSQIRTPTLVISGTHDPAATPA
                     DGRFLAERIPGARYAELNTSHLSNVEAPAQFNDAVLSFLNA"
     gene            3143..3532
                     /gene="pcaC"
                     /locus_tag="VFR42_06155"
     CDS             3143..3532
                     /gene="pcaC"
                     /locus_tag="VFR42_06155"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR02425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEU0368770.1"
                     /translation="MDERKRYDEGMKTRRSVLGDAHVERTLKNRTKFNEPFQDLITRY
                     AWGEIWNRPGLPKKTRSLLTLAMLVALNRGEEFRMHVKAALSIGVSRDEIQELLLQAA
                     IYCGVPAANSAFHDAEEVFDVLDKKKS"
BASE COUNT          727 a         1306 c          927 g          781 t
ORIGIN      
        1 cgaaaagctg ggaaggcagc tggacggcgc gcgccagaaa cggaggcgcg ctctctggga
       61 cctggagcgc aaatttaggg gatttcaagg gcaagacgat gcaggagatg ctggcgctga
      121 cgatcgaaaa gcagatcggg ggagcatggc agagtggacg ttatcaagga aattggtggc
      181 tggagggcag ccgcaagaaa aagcggacgg ttcgcgaccg acgacgaaac tggagcacac
      241 aagggacgtt cgatgcggat agcgattcat ttctgtttgt tttgccggtt tgtttcccag
      301 tccgattttc gttagcgacg ttactgggaa agggcagaat tctcgccgcg cagggagcaa
      361 gtctcgcgca ggtggcgaga aaacacgaat cggcgcctct ggaatttact taccgggata
      421 agccagatgg aaacgagtta tttcgcaaag cgcgctttga cttcctccgg aatcgcctct
      481 ccccgcattc ctttttccga gctcgcgtcg cgcacggtcc atacgcgttt ctcgcgccct
      541 tccgcgccca gcacgtttcc tttgtatagc gcgtgttcca cacgaaaact gctcttcccc
      601 cactccacaa tccggctctc gacaaccacg gtgtcgccga acgacgccgg cgcataaaac
      661 tgcgcgctcg cttccaccag cggaattccc accaatccat agtatttaag catctctgcc
      721 ttgcgaaaac ctgccgcttc aaacagcgca ttggtgcacg catcgaaata ctcaaaaaat
      781 ctcgggaagt acacaatccc cgccgggtcg cagtcgcccc attccacgcg aatctctttg
      841 cggttgctta gcaccatttc gctcctcgca atctcgtgga gtctaccgta tcgccaccgt
      901 cgcgcggaat ccgtttttct tacatcggct tcccaattac actctagcca tgcaacttct
      961 ggatggcttc ttccgctcgc ccaccgttga gcctttcttc accgatgccg ccgccgtcca
     1021 gtccattctt gattttgaag ccgctctcgc ccgggcgcag gcccgggcag gactgatctc
     1081 ggaaaatttc gcctccacga ttgcctcttt ctgccgcgcg gaactcgttg atctgccagc
     1141 cctcgcgcag gccatgccgt ccgccggcaa tctcgccatc ccgctcgtca aacaactcac
     1201 cgccagcgtg gcgcgcactt ctcccgacgc cgcgtgtcac gtccactatg gcgccaccag
     1261 tcaggacgcc ctcgacaccg gcctggtgct gcagttgcgt tccgcaaccc gcgcgcttca
     1321 cgctgatctc gaaacaatta tttcttcttt ggccgagctg acttccgcgc atcgcaaaac
     1381 cttgctggtc gcccgcacct ggttgcaaca cgccttgccc accactttcg gcttcatcac
     1441 cgccggttgg ctggacgcct gccttcgcca tcgtgaacgc ctgaacgctc tgctcgaaca
     1501 ttcgctggtg ctgcagttcg gcggcgccgc aggcaccctc gccgctcttc gcgatcgcgg
     1561 cgcactggtt gcgtctctgc tggcagaaga gctggctctc cccttgccgc gcattccgtg
     1621 gcacgcgcaa cgtgaacgca tcgccgaagt ggcgaccacc tacgggcttg tctccggcac
     1681 gttcgcaaaa attgcgcgcg atctttcgct gcacatgcaa accgaagtcg ctgaactcgg
     1741 cgagccgacc gcgaccggcc gcggcggctc ctccaccatg ccgcacaagc aaaatccagt
     1801 cgcctgcgcg gccattcttg catcgacttc ccgcgtcccg ccactcgtct ccacaattct
     1861 ctttggcctc tccggtgaat atcaccgcag cctcggtccg tggcagtccg agtgggaaac
     1921 actcccggaa atcgtccgcc tctctgccgg cgcctcgcac cacctcgccg ggctgcttcc
     1981 gcgcctcacg atacactccg atcgcatgcg cgccaatctc gaactcaccc acggcctcat
     2041 ctatgccgaa gccatcacgc tcgccctcag cggaacgttg aatcgcgcct ccgctcacaa
     2101 gctcgttgaa gaggcctgcc gccgtgcgca atccgagcgc cgccatcttc gcgaaattct
     2161 ttctgccgac gctgaagtca acgccattct cgacgcgcag tccatcgcgc aactcttcga
     2221 gcctgccaac tatctcggct ccgcgggagc cgcaatcgaa actgttctgt ccgccgcgaa
     2281 atcgcagcct cgctccgcca ctaaatccaa cgccacggga taagccatgc cctttgctaa
     2341 tctgccgaac gtccgcttgc actacgcgct ctcaggactc ccagacgctc ctgtgctgat
     2401 tctttccaat tccctcggca ccaacttttc catgtgggac gggcaagctc ccgccttcga
     2461 aaaacagttt cgcctgctgc gctacgacat gcgcggccac ggccaatctt ccgcaccgcc
     2521 tccgccctac gccgttcctg acctcgcctg cgacatcctc tccctcgccg attcgctttc
     2581 catcccacgc ttccattttt gcgggctttc catcggcggc atgatcggca tgtcgctcgc
     2641 tttccaggcg cccgagcgtc tgcacaagct tgtgctctgc aacaccgcgc ccaaaatcgg
     2701 cgcactcgaa ttctggaaca ctcgcatcca gaacgttcag tccaaaggta tgaaagaagt
     2761 cgccggcgcc gtcaccgccc gctggtttac tccaggcttc cagcagtcct ctcccgaaaa
     2821 agttgcttcc gccattcgcg tcctcgaatc gctcgatccg caaggatatg ctggcggctg
     2881 caccgccgtc cgcgatttcg actttcgcga aagtgtcagc caaatccgca cgccaacgct
     2941 ggtcatctcc ggcactcacg atcccgctgc cacccccgcc gacggccgct tcctcgccga
     3001 acgaatcccc ggcgcgcgct acgcagaact gaacacctcg cacctttcca acgtcgaagc
     3061 cccggcgcaa ttcaacgatg ccgtcctttc ttttttgaac gcctgacccg cggcaatttt
     3121 gcagcaattc cttggaggaa ttatggatga gcggaaacgc tacgacgaag gaatgaagac
     3181 gcgccgctcg gtgctgggcg acgctcacgt agagcgcacc ctaaaaaatc gcaccaagtt
     3241 caacgaaccc ttccaggacc tgatcacccg ctacgcctgg ggcgaaatct ggaaccgccc
     3301 cggcctgccc aaaaagacgc gcagcctgct cactctcgcc atgctggttg cgctcaaccg
     3361 cggcgaagaa tttcgcatgc acgtcaaagc cgcgctttcc attggagtgt cgcgggacga
     3421 aatccaggaa cttttgctac aggccgcaat ctactgcggc gttcccgccg ccaattccgc
     3481 attccacgat gccgaagagg tcttcgacgt gctagacaaa aagaaatcgt aattcaatta
     3541 gggaaaatct tcgccgaaga ccggcgaatc tacacgacgg cgaacctagt ccggcttagc
     3601 cacttccgta cgcgcattct tcttttcgtc cagcttggcg ataatctctt tctgaatcgc
     3661 aaactctttc tgcgcctctt ccatgtgtcc cgttcggctc agcgccatcc ccaaattgta
     3721 gtgcgccgac ggatttcccg g
//
LOCUS       DASTPM010000354         4469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_387433, whole genome shotgun sequence.
ACCESSION   DASTPM010000354 DASTPM010000000
VERSION     DASTPM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387433"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VFR42_06160"
     CDS             complement(<1..249)
                     /locus_tag="VFR42_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0368771.1"
                     /translation="MTLAFLAIYVIWGSTYLAIWYAVASIPPLYTAGLRHAAAGTILL
                     LWALAKGQRPTWPQIRASIVIGFLFFLVGHGTLHWAETQ"
     gene            462..1037
                     /locus_tag="VFR42_06165"
     CDS             462..1037
                     /locus_tag="VFR42_06165"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEU0368772.1"
                     /translation="MPLTHAQFLAKGHGKTAPWLDLVNSEEWDTYGKLTDWLDDPSWL
                     PFFLRQWHFTAPDHSRFPAAKFKALRAALRKSCEALFTERVIPAQILHAINHALSLSG
                     KRALIQRQNGLQVEFLPHKSGWELILAQTALSFAETVAPQKSGRIKICLNSDCRWIFY
                     DATKGRTRRWCSDKVCGNRDRVRRARARMSS"
     gene            complement(1050..1634)
                     /gene="folE"
                     /locus_tag="VFR42_06170"
     CDS             complement(1050..1634)
                     /gene="folE"
                     /locus_tag="VFR42_06170"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004912347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU0368773.1"
                     /translation="MDEAKPYDERRVACHMREIIRLVGEDPHREGLRKTPERFEKALK
                     FLTSGYNQNVDHILNGATFSVSYDEMVVVKDIEFFSLCEHHVLPFFGKAHVAYLPSNK
                     VLGLSKIARLVNMFARRLQIQERMTSQIAKAIEEKIAPQGVGVIIEARHLCMQMRGVE
                     KQHGSAVTSAMLGAFRHNKQTRDEFLALVGGKWG"
     gene            complement(1699..2121)
                     /locus_tag="VFR42_06175"
     CDS             complement(1699..2121)
                     /locus_tag="VFR42_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU0368774.1"
                     /translation="MSHGLHIELGRRYRFAASHRLHNAQLSEEENLRIYGKCNNRYGH
                     GHNYTVEVRFSGAVDPATGMIANLADLDAYVRERVIEPFDHRSLQDEVPVFREVVPTT
                     ENLCVEIYERLKSFPRAKLEAVRIEETSNNSFEYAGKA"
     gene            complement(2121..2546)
                     /locus_tag="VFR42_06180"
     CDS             complement(2121..2546)
                     /locus_tag="VFR42_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU0368775.1"
                     /translation="MVYLTRKVEFAASHRYHNPAFSAEENRRVFGKCNNPHGHGHNYR
                     LEVTIAGEPDPVTGMVLDLKELKDILEREIMQRMDHRFLNYEVAELKGQIPTCENVAR
                     VIWNLLEPKIPQGKLHRVRLYESADLFADCYRNGTGGAS"
     gene            complement(2552..3577)
                     /locus_tag="VFR42_06185"
     CDS             complement(2552..3577)
                     /locus_tag="VFR42_06185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0368776.1"
                     /translation="MTASGAISAIVPARNEEAVIAACIESLAAQREIGEIIAVNDQSS
                     DRTQEILQELAQRIPTLRVVEAAVPPPGWVGKNHACWIGAQQAEGEWLLFTDADAVLS
                     EGAAGKALALAAQHQASLVSFSPEQVLDNWYEKALIPFVYVRLAQKISFDQVNDPESD
                     AAAANGQFLMVRRDTYQTAGGHAALHGEVLEDVAFAQRVKGHGGRIWFGYSDGAVRVR
                     MYRSFSAMWEGWKKNLFVLMGGTTGSVFSEFESTFPWMIIVVLLIGIKMPIALFAGVV
                     LLLLRQLSYGLALTRNQFPFKFIVYYLPAVFLYAGALWASHESYAKGKVSWKGREYPV
                     GSPGASK"
     gene            3636..4178
                     /locus_tag="VFR42_06190"
     CDS             3636..4178
                     /locus_tag="VFR42_06190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0368777.1"
                     /translation="MERFRRVLVWTGLALGFSLLVLFALPSYRQGEASIAGKTAEDFA
                     LTVDNKPLRLSDYRGKIVVLNFWASYCQPCVEEAPSLNRLQRYLEPRGGTILGVSIDP
                     EPTGYEKFLKNFSVPYPTWRDPNLQDGKSKLALDYGTTLIPETYVIDRSGKIARKLVS
                     AQQWDSPEMLAYFDAILKEK"
     gene            4281..>4469
                     /locus_tag="VFR42_06195"
     CDS             4281..>4469
                     /locus_tag="VFR42_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HEU0368778.1"
                     /translation="MKTSLFEIFKIGIGPSSSHTVGPMRAAAAFTSSLHDSGLLSETA
                     RVEANLYGSLALTGRGHGT"
BASE COUNT          953 a         1430 c         1125 g          961 t
ORIGIN      
        1 ttgcgtttcc gcccaatgga gcgtgccgtg accaaccaga aagaacagga acccgatgac
       61 gatgctggcg cgaatctgcg gccacgtcgg gcgctggccc ttggccagag cccagagcag
      121 cagaatcgtt cccgctgccg cgtggcgaag tcccgcggtg tacaacggcg gaatggaggc
      181 aacggcatac cagatcgcca ggtacgtcga gccccaaatc acataaatcg ccagaaaagc
      241 cagggtcacc gtgaaacgac cggccggaac cgctgtgcgg ctgggtgccg ggacctcgcg
      301 agcccgttca ggccgcggta aggttgccgt tttggcagag gcactcacta agttctccgt
      361 ctggcgtatc gttctaacgc gcgtaacgtc ctaattgact tatactcgtt accatgctaa
      421 cctacgcctt gtaacgagtc aactcatttt aaggcattac tttgccactg acacacgcgc
      481 aattcctcgc caagggccac ggcaaaaccg cgccgtggct ggacctggtg aatagcgagg
      541 agtgggatac ctacggaaag ctcaccgact ggctggatga cccgtcctgg cttcccttct
      601 tcctccggca atggcacttt acggcgccag atcattcgcg ttttccggcg gcgaaattca
      661 aggccttgcg cgccgcactt cgcaaaagct gcgaagcact cttcacagaa cgcgtcatcc
      721 ctgctcaaat actacacgca ataaatcacg cgttgagcct atccggcaag cgcgctctga
      781 tacagcgcca aaatgggttg caggttgaat ttctgccgca caaaagtggt tgggagttaa
      841 tcttggcaca gaccgccctc tctttcgcgg agacagtcgc gcctcagaaa tcgggaagaa
      901 tcaagatttg cttgaatagc gattgtcgat ggatcttcta cgatgctacg aaaggccgca
      961 cccgccggtg gtgcagcgac aaagtctgcg gcaaccggga tagagtacga cgcgcccgcg
     1021 cccgtatgtc gagttgaatc ggctggaagt cagccccact tgccgccgac cagcgccagg
     1081 aattcatctc gcgtctgctt gttatgccgg aaggctccca gcatcgcgct agtcaccgcc
     1141 gaaccgtgct gtttttccac cccgcgcatc tgcatgcaca ggtggcgcgc ttcgatgatc
     1201 acacccacgc cctgcggtgc aatcttttcc tcaatggcct tcgcgatttg gctggtcatc
     1261 cgttcttgaa tttgcagacg gcgcgcaaac atgttcacca gccgagcaat cttgctcagg
     1321 cccagcactt tgttgctggg cagatacgcc acgtgcgctt taccaaaaaa cggcagcacg
     1381 tgatgctcgc acagactgaa aaattcgatg tctttcacca ccaccatctc gtcgtacgaa
     1441 acactgaatg tcgccccgtt cagtatgtga tccacattct ggttgtagcc gctggtcaga
     1501 aacttcagcg ccttttcaaa ccgctccggc gttttgcgca atccctcgcg gtgcgggtct
     1561 tcgcctacca ggcggataat ctcccgcatg tggcaagcca ctcgtcgctc atcatacggc
     1621 ttcgcttcgt ccagaataat ttcttcgctt ttggattcca cgggcttgct cacggcgcgc
     1681 tccccttttc ctcaacgctt acgctttccc tgcatactca aaactgttat tggaagtctc
     1741 ttcgatgcgt acagcctcca gcttcgccct tggaaaagat ttcagtcgct cataaatttc
     1801 cacgcacaaa ttttccgtgg tcggcactac ctcgcgaaat accggcactt catcttgcag
     1861 cgaacggtga tcgaacggtt caattacccg ttcgcgtaca tacgcgtcca ggtccgccag
     1921 gttcgcaatc attccggtcg ctggatccac cgcaccggaa aaacgtactt ccacggtgta
     1981 attgtggccg tgcccgtacc gattgttgca ctttccgtag attcggagat tttcttcctc
     2041 gctcaactgc gcattgtgca gacgatgcga cgcggcgaaa cgataccgtc ggccaagttc
     2101 gatatggagt ccatgcgaca tcagctcgct ccgccggttc cgttgcgata acaatccgcg
     2161 aaaaggtctg ccgattcgta aagtcgcacg cgatgcagtt taccctgcgg aattttcggc
     2221 tccagcaaat tccagataac acgggccacg ttttcgcagg tcgggatctg acccttcagc
     2281 tccgcgacct catagttgag aaaacgatga tccatgcgct gcatgatttc gcgttcaaga
     2341 atgtctttca gctctttcag gtccagcacc attcccgtga ccggatcggg ctcccctgcg
     2401 atcgtcactt ccagcctgta gttgtgcccg tggccatgcg gattgttaca cttgccgaac
     2461 acgcgccggt tctcttccgc cgagaacgcg ggattgtggt accggtgcga agcagcaaac
     2521 tcgactttac gcgtcagata gaccatcgat tttatttaga tgctccaggc gagcctaccg
     2581 ggtattccct tcccttccag gacactttgc ctttcgcata gctttcatgc gaggcccaca
     2641 gcgctccggc gtacaaaaaa accgccggca aatagtacac gatgaacttg aagggaaact
     2701 ggttgcgagt cagcgccaaa ccgtaagaaa gctgccgcaa caacagcagc accacgccgg
     2761 caaacagggc aatcggcatt ttgattccga tcagcagcac cacaataatc atccacggaa
     2821 acgtcgattc aaactccgaa aacaccgatc ccgtcgtgcc gcccatcaac acgaatagat
     2881 ttttcttcca gccttcccac atcgctgaaa atgagcggta catccgcact cgcacggctc
     2941 cgtcgctgta gccaaaccag attcgcccgc cgtggccctt cactcgttgc gcgaacgcga
     3001 catcttccag cacttcacca tgaagcgccg catgtccgcc ggctgtctga tacgtatcgc
     3061 gccgcaccat caaaaattgc ccgttcgccg ccgcggcgtc ggactccgga tcgttcacct
     3121 ggtcaaacga gattttctgc gccaaccgga cgtaaacgaa cggaatcagc gccttctcgt
     3181 accaattgtc caacacctgc tccggcgaaa acgaaacgag cgatgcctga tgctgggcgg
     3241 caagcgccag tgccttcccc gctgcgcctt cgctcagcac cgcatccgca tccgtaaaca
     3301 gcagccattc gccctccgcc tgctgcgctc ctatccagca tgcatggttc ttccccaccc
     3361 atccgggtgg aggaaccgcc gcctcgacga cccgcaacgt cggaatgcgc tgcgcaagtt
     3421 cctgcaaaat ctcctgcgtg cggtcgctag actgatcgtt caccgcaatg atttccccga
     3481 tttcccgctg tgccgccagt gactcaatgc aagccgcaat cactgcttct tcattgcggg
     3541 cagggacaat cgcgctgatg gctccggaag cggtcataag ggtggggttg ggaagcgttt
     3601 cggtacccgc gccgatacgt tattataaca ctcgtatgga acgcttccgc agagtgctgg
     3661 tctggaccgg tttggctctg ggcttctcgc tcctggtcct ctttgccttg cccagctacc
     3721 gccagggcga agcctcgatc gccggcaaaa ccgccgaaga tttcgcgctc acggttgaca
     3781 acaagccgct ccgcctttcc gattatcgcg gcaaaatcgt cgtactgaat ttctgggctt
     3841 cctactgcca gccctgcgtc gaggaagcgc catcgctgaa tcgcctgcag cgctacctcg
     3901 aaccgcgcgg cggcaccatc ctcggtgtca gcatcgatcc ggaacccacc ggctacgaaa
     3961 aatttctcaa gaatttcagc gtgccttacc ccacttggcg cgaccccaac ctgcaggacg
     4021 gcaaatccaa actcgcgctt gattacggca caacgctaat tcccgaaacc tacgtcattg
     4081 accgcagcgg caaaatcgcc cgtaagctcg tcagcgccca gcaatgggat tcccccgaaa
     4141 tgctcgccta ctttgacgcc attttgaagg aaaagtagcc gctcggcagc cgtcgcaagg
     4201 gcacgtgggc acactccgcg attgccatgc tttcgccgaa cgtcaggcga gcgtagcatc
     4261 atagttgggt aaatactccc gtgaaaacca gcctgttcga aatcttcaag atcggcattg
     4321 gcccgtcgag ttctcacacc gtcggcccca tgcgcgccgc agcagctttt acctcgagcc
     4381 tccacgattc cgggctgctg tcggaaacgg ctcgcgtgga agctaatctc tacggctcgc
     4441 tcgcgctcac cgggcgcggt cacggcacc
//
LOCUS       DASTPM010000355         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_531553, whole genome shotgun sequence.
ACCESSION   DASTPM010000355 DASTPM010000000
VERSION     DASTPM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531553"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFR42_06200"
     CDS             <1..477
                     /locus_tag="VFR42_06200"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU0368779.1"
                     /translation="CALGAGFRAVGQGLRVLMVQFIKGSWHYGELDTAKMLGDDRFEI
                     RPMGRGFVKIGGAETDPQDIKLAEECWQTGLDAIHSGKYELVILDEINYVISYKMLDA
                     DKIVEALRGKPERVHVICTGRNAHPKLVELADLVTEMKEVKHPYTKGILAQRGIDY"
     gene            618..1466
                     /gene="accD"
                     /locus_tag="VFR42_06205"
     CDS             618..1466
                     /gene="accD"
                     /locus_tag="VFR42_06205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU0368780.1"
                     /translation="MAWFKREKKAIAEATPPEERRVRTEGLWTKCENCRTITFRKDLE
                     ENLFVCPKCQFHFKMSAKQRLEMLLDSRWTEHDAGMTSTDPLKFVDTKPYASRIKEAR
                     KKLGVNDAVITAEGQLNGRPVVVCAMEFGFIGGSMGAVVGEKVTRGIELAMDTRQPLI
                     VVSCSGGARMMEGTISLMQLAKVSAALARLDEAKLPYISVLTDPTTGGVTASFAMLGD
                     LNIAEPGALIGFAGPRVIEQTIRQKLPEGFQRSEFLLEHGFLDAIVPRKELKSFITSS
                     LDLLMN"
     gene            1468..2811
                     /locus_tag="VFR42_06210"
     CDS             1468..2811
                     /locus_tag="VFR42_06210"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEU0368781.1"
                     /translation="MNFQEAVNYLLSLGRELAAPTQAATAKFNLENISVLARHLGHPE
                     NKYASVHIAGTNGKGSTAAFLEAMLREAGYRTGLYTSPHLERINERIRVEGNDIADGA
                     FAEVFSRIHALIEKLLADGTLRAHPTFFECVTAIAFLYFAEAGVDFAVFETGLGGRLD
                     STNIVTPQVSIITRIDFDHENFLGHSLREIAAEKAGIIKPAVPVVIAKQRDEAREVIA
                     SRASELHSPLVEPATLYAVESLRMEEGRVRAVVREPRAGKSLNIDPQLAGRFQLHNAL
                     NAVAAAHVLKPQGYRLTDGVIELGIATAVWPGRIEKIRVRPDIYLDGAHNPASARELD
                     AFVAENFAGRRIILIFGALRDKAVDEIAGILFPRAAHVIFTEPATSRAISAPQLAEIA
                     GHYATSAEIIPDAAQALEAALAKAQPGDAIFITGSLYLVGQLRRAAQAATSAASG"
     gene            2888..3331
                     /locus_tag="VFR42_06215"
     CDS             2888..3331
                     /locus_tag="VFR42_06215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0368782.1"
                     /translation="MSNQEPTTEPTLFERMMKHRAPVGDLVGFEVPEAGNGRTVVTLQ
                     SGPQHFNPMGTLHGGILCDIADTAMGVAFASTLAPGESFTTVELKINYLRPVREAKLR
                     AEGRVVQRGRTIGYVECEVTDENGKLIAKSNSTCLVLRGEQASGR"
     gene            3437..>3838
                     /gene="purE"
                     /locus_tag="VFR42_06220"
     CDS             3437..>3838
                     /gene="purE"
                     /locus_tag="VFR42_06220"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU0368783.1"
                     /translation="MAAKSKGAQRKSLVGIIMGSTSDWETMEHAANTLKNLGVPYETR
                     VVSAHRTPDLLFSYASQAEGRGIEVIIAGAGGAAHLPGMTASKTVVPVLGVPVESKAL
                     KGLDSLLSIAQMPGGIPVGTLAIGKAGAINAA"
BASE COUNT          815 a         1201 c         1060 g          762 t
ORIGIN      
        1 tgcgccctgg gcgcagggtt tcgcgctgtt ggccaggggc ttcgcgtgct gatggtccag
       61 tttatcaagg gctcgtggca ttatggcgaa ctcgacaccg ccaaaatgct gggcgatgac
      121 agatttgaaa ttcgaccgat gggccgcggc ttcgtgaaaa tcggcggcgc ggaaaccgac
      181 ccgcaagata ttaagctcgc ggaagaatgc tggcagaccg gcctcgacgc cattcactcc
      241 ggcaaatacg agctggtgat cctcgacgag atcaactacg tcatcagcta caaaatgttg
      301 gacgccgaca aaattgtcga agccctacgc ggaaaaccgg agcgcgtcca cgtcatctgt
      361 accggccgca atgcacatcc caagctcgtc gaacttgccg acctggtcac cgaaatgaaa
      421 gaagtgaagc acccctatac caagggcatt ctcgcccagc gtggtataga ctattgaagc
      481 ctggatggca gccggcttat ttggcgcagt gtcccaagga ttttgtgtag cgtcgggctt
      541 tagcccggca tcttcgcggt taatccgggt ccgtggcttg ggtgcgagct atggcagcag
      601 aaagaaaaca tcgcgaaatg gcctggttca aacgggaaaa aaaggcaatt gcagaggcga
      661 ccccgcccga ggaacgccgc gtccgcaccg aaggtctgtg gaccaagtgc gaaaactgcc
      721 gcactatcac ctttcgcaaa gacctcgaag aaaatctttt tgtctgcccc aagtgccagt
      781 tccatttcaa aatgagcgcc aagcagcgct tggaaatgct gctggacagc cgctggaccg
      841 agcacgacgc cggaatgacg tccaccgatc cgctgaagtt cgtggacacc aagccctacg
      901 cttcgcgcat caaggaggcc cgcaaaaaac tgggcgtcaa cgatgcggtc atcaccgccg
      961 aaggccagct caacgggcgt ccggtcgtgg tctgcgcgat ggaatttggc ttcatcggcg
     1021 gctccatggg cgcggtggtt ggagaaaaag tcacccgcgg catcgaactc gccatggaca
     1081 ctcgtcagcc gctcatcgtc gtttcctgtt ccggtggcgc tcgcatgatg gaaggcacca
     1141 tcagcctgat gcaactggcc aaagtttccg cggcgctggc gcgtctcgat gaagccaagc
     1201 tcccctacat ttccgtgctg accgatccaa cgaccggcgg cgttaccgcg agtttcgcca
     1261 tgctgggtga tttgaatatc gccgaacctg gagcgctgat cggcttcgcc gggccgcgtg
     1321 tcatcgagca gaccatccgg cagaaattgc cggaaggctt ccagcgctcg gaatttctgc
     1381 tcgaacacgg ctttcttgac gccatcgtcc cccgcaagga actcaagtcc ttcatcacct
     1441 cttccctcga tctcttgatg aactgaaatg aattttcagg aagcggtgaa ctatctgttg
     1501 tcgctggggc gcgaacttgc tgcgccgact caggctgcga ccgcaaagtt caacctcgaa
     1561 aacatttccg ttctggcgcg gcatctcggc catcccgaaa acaaatacgc ctcggtgcat
     1621 attgccggaa ccaacggcaa aggctcgacc gccgcttttc tggaagccat gttgcgcgag
     1681 gccggctatc gcaccggcct ttacacgtcg ccgcatctcg aacgcatcaa cgaacgcatc
     1741 cgcgttgagg gcaacgacat tgccgatgga gcgttcgcgg aagttttctc tcgcatccac
     1801 gcgctcatcg aaaaactgct ggctgacggc acgctgcgcg cgcacccgac tttttttgag
     1861 tgcgtcaccg ccatcgcatt tctctatttt gccgaagctg gggtcgactt cgccgtgttc
     1921 gaaacggggc tcggaggccg cctggattct accaacatcg tcacgccgca ggtcagcatc
     1981 atcacccgaa tcgatttcga tcacgaaaat ttcctcggcc actcgctgcg tgaaatcgct
     2041 gcggaaaagg ccggcatcat caaacctgct gtaccggtgg tgatcgccaa acagcgcgac
     2101 gaggcgcgcg aagtcatcgc ttcgcgagcc agcgaacttc actcgccctt ggtggaaccc
     2161 gccaccttat atgcagtcga gtcgctgcgc atggaggaag gccgcgttcg ggccgttgtc
     2221 agggaaccca gagccggcaa atctttaaat attgatcctc aacttgccgg ccgcttccag
     2281 ctgcacaacg cgctgaacgc cgtcgcggcg gcgcacgtct tgaagccgca aggttatcgc
     2341 ctgacggacg gcgttatcga actgggaata gccacggcgg tctggcccgg ccgaatcgaa
     2401 aaaatccgcg tgcgaccgga catttacctg gatggcgccc acaaccccgc ttcggcccgc
     2461 gaactggacg cctttgtcgc cgaaaacttt gccggccgcc ggatcatcct gatttttggc
     2521 gcactccgcg acaaagctgt agacgaaatc gccggcattt tgtttccacg cgcggctcac
     2581 gtcattttca ccgagccggc cacttcgcga gccatttccg cgccgcaact tgccgaaatc
     2641 gccggccact acgcgacatc tgctgaaatc attcccgatg ccgctcaagc actggaagct
     2701 gcgctggcca aagcgcaacc aggtgacgcc attttcatta ccggttcgct ttatcttgtt
     2761 ggacagttgc gccgggccgc gcaggccgcc acttccgccg caagcggtta ggtcgcttgc
     2821 aacagttcgc agaacaattt cgcgtatgct gcatccaact gaacactcaa aatcgcaagg
     2881 agcctctatg tctaatcaag agccaaccac ggaacctacg ctcttcgaac gcatgatgaa
     2941 gcaccgtgcg ccagtgggcg acctggtcgg ctttgaagtc cccgaggccg gcaatggccg
     3001 caccgtagtc accttgcaat cggggccgca gcacttcaat cccatgggca cgctgcacgg
     3061 cggcatcttg tgcgacatcg ccgacaccgc gatgggcgtg gcttttgcca gcacgctcgc
     3121 tcccggcgaa tcgttcacta cggtcgaact caaaatcaac tacctgcgtc ccgtgcgcga
     3181 agccaaactc cgcgccgaag gccgcgtcgt ccagcgcggc cgcaccatcg gctacgtcga
     3241 atgcgaagtc accgacgaaa acggcaagct catcgccaag tccaactcta cctgtctggt
     3301 cctccgcggc gagcaagcct ccggtcgtta acctcgttat ttacccagaa tcgtaacgtc
     3361 gcatcttagc cggctggccc tcaatcctgg gacgggtttt ccaattcgtt gcctgcgcga
     3421 tagacttgtc cctctcatgg cggctaagtc caaaggcgca caacgtaagt cgctggtcgg
     3481 tatcatcatg ggctccactt ccgattggga gaccatggaa catgccgcaa acacgctgaa
     3541 gaatcttggc gtaccgtatg aaacgcgcgt ggtttcggcg caccgcactc ccgacctgct
     3601 tttctcctac gcttcccaag ccgaaggccg cggtattgaa gtcatcatcg ccggtgcagg
     3661 tggagcggcg cacctccccg gaatgaccgc ctccaaaacg gttgttccgg ttcttggcgt
     3721 tccggttgaa tcgaaagcgc taaaagggct ggactcgctg ctttccatcg cgcagatgcc
     3781 gggtgggatt cccgtaggca cgctggccat cggaaaagcg ggggcaatca acgccgcc
//
LOCUS       DASTPM010000356         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_819790, whole genome shotgun sequence.
ACCESSION   DASTPM010000356 DASTPM010000000
VERSION     DASTPM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819790"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VFR42_06225"
     CDS             <1..1415
                     /locus_tag="VFR42_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368784.1"
                     /translation="LSDYWRGFIAGLIWLSFCGTFLLARIVMPEPLVSAFIAGAVFCG
                     LSGYERRRRRSVWFAGVWIFSALACLTKGLLGIVYPAAILVLLSIFYRQARIRFRGLL
                     RWEYAAIFLLIVTPWHIWAQWHFPHYFRFLIRSEWLGHLRGLTDETHDFLGVPGYQFV
                     ILHFAWWFPWSIALLPGVIFAWRRVIRPREINFGDGLLLCWMGVVFVPLLFLGQRQDY
                     YSMSMWSAFALWAAVAWDRTPQGLRAAGVIAVALGGVAAAAGAVFLAAAARAPNGNWG
                     VMDARWTAWKALHDMPVSAWVALRPLIAITAISFVFFSIVALYFIFRRREKLAAIAVA
                     LSMIPAGLSMVVGVAHAAPYFSLADVARFLNPRLETGGHAFFEGPLDNSSSLIFYLNR
                     KFFFVNQNPRKEAPIGTPASDIFLDEQTVLDRWGRAEAVYLIVEQSRVDYWKERVTSR
                     FHIYHQVTKCGTYVVLSNQL"
     gene            1519..1674
                     /locus_tag="VFR42_06230"
     CDS             1519..1674
                     /locus_tag="VFR42_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HEU0368785.1"
                     /translation="MSQHRSLKGASTIAAKRNVLKRFERVEVLKKRGQWKDGQKVIGL
                     PKTKPDV"
     gene            1747..>2117
                     /locus_tag="VFR42_06235"
     CDS             1747..>2117
                     /locus_tag="VFR42_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU0368786.1"
                     /translation="MNRYLAAAGVGSRRQCDELIAAGRVTINGKICTNFNAQPGARDH
                     VKVDGKLVRAAPPLTIMLHKPAGFVSTRRDPHVRDTVFDLLPAKFSRLFNIGRLDAQT
                     EGLLLLTNDGELAQRLTHPRY"
BASE COUNT          399 a          577 c          618 g          523 t
ORIGIN      
        1 agctgtccga ttactggcgc ggtttcattg ccggtctgat ttggctcagc ttctgcggga
       61 cgtttctgct ggcgcgcatt gtgatgcccg agccgctggt cagcgcgttc atcgccggag
      121 cggttttctg tggactatcc ggttatgagc gcaggcggcg tcgcagcgtg tggttcgccg
      181 gtgtttggat tttttccgcg ctggcttgcc tcaccaaagg tttgctcggc atcgtttatc
      241 ccgctgcgat tctcgttttg ctctcgattt tttatcgcca agcaaggata cgctttcgcg
      301 ggctgctgcg gtgggaatat gcggcgattt ttcttttgat cgtgacgccc tggcacatct
      361 gggcgcaatg gcatttcccg cattattttc gcttcttgat caggtccgaa tggctgggac
      421 acctgcgggg acttaccgat gaaacgcacg attttctggg agtgcctggg taccaatttg
      481 taatcctgca tttcgcctgg tggtttccat ggtccatcgc gctgctgccg ggcgtgattt
      541 ttgcctggcg ccgcgtgata cgcccgcgcg agatcaattt cggcgatgga ttactgcttt
      601 gctggatggg cgtggtgttc gttccattgc tcttcctggg ccagcggcag gactactatt
      661 ccatgagcat gtggagcgca tttgcgctgt gggcagccgt cgcatgggac cgaaccccgc
      721 agggattgcg cgcggcaggc gtgatcgcgg tggctctcgg cggtgtagca gccgccgcgg
      781 gagcagtgtt tcttgccgcg gcagcgcgcg ctccaaacgg aaattgggga gtaatggacg
      841 cgcgctggac cgcctggaag gcactgcacg atatgccagt gtccgcgtgg gtagcactgc
      901 ggcctttgat tgcgattacc gctatctcct tcgtgttttt ctcgattgta gcgctctact
      961 ttattttcag acggcgcgag aaactcgcgg ccatcgcggt ggcgctgtcg atgattccgg
     1021 ctggactcag catggttgta ggcgtagcgc atgccgcacc ctatttctca ctcgccgatg
     1081 tagctcgttt tctgaatccg cggctcgaga caggcggtca cgcgtttttc gaagggccgc
     1141 tggacaacag cagcagcctt attttttatc tgaaccgcaa attctttttt gttaatcaaa
     1201 atccccggaa ggaagcaccg atcggcacac cagctagcga catcttcctg gacgaacaga
     1261 cggtcctcga cagatggggt cgcgcagagg cggtttacct cattgtggaa cagagtcggg
     1321 tggactactg gaaagagcga gtaaccagcc ggtttcacat ttatcaccag gtcactaaat
     1381 gcggcacgta cgtcgtgctt agcaatcagc tctaagtgaa aagtgttccg tgtgtttctt
     1441 gaaaaacttc cgcgatagcc taatgtggcc gttcggtttc cgtcgtaacc atttaactgt
     1501 ttaactttca acccggccat gtcgcaacac cgcagtttaa aaggggctag taccattgcc
     1561 gccaagcgca acgtgctgaa acgcttcgag agggtcgaag ttctgaagaa gcgcggtcag
     1621 tggaaggacg gtcagaaagt catcggcctg ccgaagacca agccggacgt ttaggaccgt
     1681 tgaaacgttt gacgggtgaa gcgttgaagc ggcgctccgt taacgcttca atagagcgcg
     1741 atgcgcttga accgctacct cgccgctgcc ggagtcggat cgcgccggca gtgcgacgaa
     1801 ttgatcgcag caggccgcgt taccattaac ggcaaaatct gcacaaattt caacgcgcag
     1861 ccgggcgcgc gcgaccacgt caaagtggat ggtaaacttg ttcgcgcggc cccaccgctc
     1921 accatcatgt tgcataaacc ggccggattt gtttcgactc ggagagatcc gcacgtccgc
     1981 gacactgtct ttgatttgct cccggcaaaa ttttcgcggc tattcaacat cggacgcctg
     2041 gacgcgcaaa ccgaaggtct gcttcttctc acgaatgatg gcgaactcgc gcaacgtctt
     2101 acgcatccgc gttacaa
//
LOCUS       DASTPM010000357         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1136939, whole genome shotgun sequence.
ACCESSION   DASTPM010000357 DASTPM010000000
VERSION     DASTPM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136939"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..651
                     /locus_tag="VFR42_06240"
     CDS             190..651
                     /locus_tag="VFR42_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0368787.1"
                     /translation="MATEITTDEYRALAELRYRIRHFLREGDIVAQAAGLEPQQYLLL
                     LTIRGLPPGQESTIRTLAERLALKHHSVVELIDRLENHGYVRRTRGREDRRRVLVSLL
                     PRGERLLEQVARHRIGELRSTGHELVRAIDHLLQKPQTNHKKSSGKREKRA"
     gene            644..>2378
                     /locus_tag="VFR42_06245"
     CDS             644..>2378
                     /locus_tag="VFR42_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU0368788.1"
                     /translation="MPSEASATKTGPTENTAPRTGGKAWEVLGDFTTTTRLLPISALA
                     ICIGVLAAFVALALLRLIGLFTNLFYFGRWSTAMVSPVGNHLGIYSVLVPIGGALIIG
                     VMARYGSERIRGHGIPEAIEAILINGSRVEPKVAILKPISSAISIGSGGPFGAEGPII
                     MTGGAFGSMIAQLFHLTSAERKTLLVAGAAAGMSATFAAPVASVLLAVELLLFEWKPR
                     SLIPVALASATAAVLRRYLLGFGPLFPVPEHPLFIGPKGLLGCVLVGLLAGGLSALLT
                     ISVYAAEDMFQHLKIHWMWWPAIGGLAIGLGGLVFPQALGVGYDTIGGLLQGSVTTHV
                     ILGVLLVKWFIWAVSLGSGTSGGVLAPLLMMGGALGGLEAMILPNEGAGFWPLVSMGA
                     VLGGTMRSPFTSIVFAFELTHDGNVFLPLLVGSVVAHGFTVLVLKRSILTEKVARRGY
                     HLSREYAVDPLEILFAREVMRTNVAVLSAADRVTDLQLSLRGDHRQAQRLLPVVDENR
                     QLVGVITRGDIRDLSSKAENRHRSLREVVRPETVSAHPDEPLRSVVYRMAEKGLTRMP
                     VVDRTTGQFLGL"
BASE COUNT          435 a          759 c          683 g          501 t
ORIGIN      
        1 ccataaattc taccttcgcc aaggttacga gcattacaaa acccagaaag ccttccgcaa
       61 gaaactatag ctccccgcgc tcgcaaccag ccttcgccat agcggcctca cgctcctaca
      121 gggttactcg cccgaaacta cggtacaacc gatttcgcga agtatatcgc agtacgatat
      181 actacaaaca tggcgacgga gatcaccacc gacgaatacc gcgctttggc cgagttgcgc
      241 taccgcatcc gccatttcct gcgcgaagga gacatcgtag cccaggccgc cggcctggag
      301 cctcagcaat accttctgtt gctgaccatc cggggtttgc cccccggaca ggaatccact
      361 atccgaacct tagccgagcg cctggcactg aaacatcaca gtgttgtaga gctgatcgac
      421 cgcctcgaga atcacggcta cgtgcgacga acccgcggcc gcgaagaccg ccgccgcgtg
      481 ctggtctccc tgctcccgcg cggcgaacgc ctgctcgaac aggtcgcccg ccatcgcatc
      541 ggcgaattgc gttccaccgg ccatgagctc gtgcgcgcca tcgatcactt gttgcaaaaa
      601 ccgcaaacca atcacaaaaa atcctccggg aagcgagaaa agcgtgccta gcgaagcatc
      661 tgcaacgaaa acgggaccaa cggaaaacac tgcgccccgc accggcggta aagcctggga
      721 agtcctcggc gacttcacca ccaccacccg gctgctgccc atttccgcat tggctatctg
      781 catcggcgtg ctcgccgcgt ttgtagcgtt ggcattgctg cgcctgattg gcctgttcac
      841 taatctgttt tatttcggcc gctggagtac cgcgatggtt tccccggtcg gaaaccatct
      901 gggaatttac agcgtgctgg tacccatcgg cggcgccttg atcattgggg tcatggctcg
      961 ctacggttcg gagcgcatcc gcggccacgg aattccagag gccattgaag ccattctgat
     1021 caacggcagc cgtgttgagc caaaagtcgc catcctgaag cccatctctt cggccatctc
     1081 catcggttcc ggcgggccat tcggcgccga aggccccatc atcatgaccg gcggtgcttt
     1141 cggctcgatg atcgcgcagc tctttcatct gaccagcgcc gagcgcaaga cgttgctcgt
     1201 cgccggagcc gccgcgggaa tgtccgcgac cttcgcggca ccggtggcct cggtcctgct
     1261 tgccgtggaa ctgctgctct ttgaatggaa gccacgcagc ctgattccgg tggcattggc
     1321 cagcgcaacc gctgctgtgc tgcgccggta tctgcttggt ttcgggccct tgtttccagt
     1381 gcccgagcac ccgctcttca tcggtcccaa gggcctcctg ggatgcgtcc tggtgggatt
     1441 gctggccggc ggcctgtccg ccctcctgac catttccgtt tacgccgcgg aagatatgtt
     1501 tcagcacttg aagattcact ggatgtggtg gcccgccatc ggcggcttgg ccatcggcct
     1561 cggcggcctg gtttttccgc aagcgttggg cgtcggctac gacaccatag gtggcctgct
     1621 gcaaggcagc gttaccacgc acgtgattct gggcgtgctt ctcgtgaagt ggttcatttg
     1681 ggccgtctcg cttggctccg gcacttccgg tggcgtattg gctccgttgc tgatgatggg
     1741 cggcgcactg ggcggactgg aagcgatgat tttgcccaac gaaggagccg gcttttggcc
     1801 cctggtcagc atgggtgcgg tgttgggcgg caccatgcgt tctcctttca ccagcatcgt
     1861 tttcgccttc gaactgacgc acgacggcaa tgtttttctt ccgctgctgg tcggcagcgt
     1921 cgtggcccac ggatttaccg ttctggtgct gaagcgctcc atcctcaccg agaaagtggc
     1981 gcgccgcgga tatcatctga gccgcgaata cgccgtcgac ccgctggaaa ttttgttcgc
     2041 ccgcgaagtg atgcgcacca atgtggcggt gctatctgca gcagacaggg tgacggatct
     2101 gcagttatcg ctgcgcggcg atcatcggca agcgcaaagg ttgttgccgg tcgtcgacga
     2161 aaatcggcaa ctggtcggcg ttatcacccg tggagacatc cgggacctct catccaaggc
     2221 tgaaaatagg catcgttcgc tccgcgaggt cgtgcgtcca gaaaccgtct ccgctcaccc
     2281 ggacgaaccg ttgcgttccg tcgtttaccg catggccgag aaagggctta cccgcatgcc
     2341 ggtggtggat cggacaaccg ggcaattcct cggactcg
//
LOCUS       DASTPM010000358         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_935043, whole genome shotgun sequence.
ACCESSION   DASTPM010000358 DASTPM010000000
VERSION     DASTPM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935043"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFR42_06250"
     CDS             complement(<1..608)
                     /locus_tag="VFR42_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0368789.1"
                     /translation="MGLNPVLLRSAVVAALGGLLFGFDTVVISGTTLSLTELFHLTPL
                     SLGVTVSSALAGTIVGSMLAGIPGEKWGRRDSLRILAVLYLISAIGCGLAWDWTALVV
                     FRVIGGLAIGGSSVLGPMYIAEIAPAKYRGRLVGLFQFNVVFGILLAYLSNYLIGTIG
                     LGGAEWRWKLGIAALPAAIFLVMLFGIPRSPRWLAKKGRVEEA"
     gene            836..2017
                     /gene="pobA"
                     /locus_tag="VFR42_06255"
     CDS             836..2017
                     /gene="pobA"
                     /locus_tag="VFR42_06255"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEU0368790.1"
                     /translation="MRTQVAIIGAGPAGLFLSLLLDRLGIQSAILELHTRQYIEERVR
                     AGLLEHNTVQLMHELGVGDRLKRQGLEHGGIELRFNNRGHRIDFRELASGKCVTIYAQ
                     HEVIKDLVAARLAANKPILFETANLQLHAVDSTSPYVTFQHNGQKSIERLDCDFIAGC
                     DGFHGVSRTSIPQGLLTTYERDYPFGWLGILAQAPPSSHELIYAHHDRGFALLSMRSP
                     QLSRLYLQCVPDENLALWPDDRIWRELQKRLATHDGWKLTEGPIIQKGVTGMRSFVVE
                     PMQYGRLYLAGDSAHIVPPTGAKGLNLAVADVRVLAVALGAYYSTGAADLLAQYSATC
                     LRRVWKVQRFSWWMTQLLHRFPDDNPFDHRRQLADLDYLTTSRAASQTLAENYVGLPL
                     P"
     gene            2341..3624
                     /gene="ispG"
                     /locus_tag="VFR42_06260"
                     /gene_synonym="gcpE"
     CDS             2341..3624
                     /gene="ispG"
                     /locus_tag="VFR42_06260"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU0368791.1"
                     /translation="MSSLTQITGFDAAATRHPRRKTYAVKVGDVQVGGGAPIVVQSMT
                     NTDTADVESTLKQTLELAEAGSEIVRWTVNVPEAAAAVPEIKQRLLAAGCNVPIIGDF
                     HYNGHVLLTKYPECAKALDKYRINPGNVGAGQRRDEQFSTICKVARDNGKPIRIGVNG
                     GSLNQELVMAKMQENTDKNLGLESGEIINQCMVLSAVQSTELALETGMRHDQIIISCK
                     TSRPRDLIMVYRDLASKTEQPLHLGLTEAGMGMKGLVWSAASLGVLLYDGIGDTIRIS
                     LTPRPGGDRRDEVYAACELLQSLGLRSFSPSVTACPGCGRTTSSTFQELAERTQDYIR
                     EMMPSWKTQYEGVEAMTLAVMGCIVNGPGESKAANIGISLPGTGEAPRCPVYIDGKKD
                     VTLEGTYDELAAAFQQLVDTYVSTKYPRKSASVSL"
     gene            3704..3871
                     /gene="gatC"
                     /locus_tag="VFR42_06265"
     CDS             3704..3871
                     /gene="gatC"
                     /locus_tag="VFR42_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU0368792.1"
                     /translation="MKISREDVVRVAELAYLDLSESELETYRAQVDEILEYIGKLNEL
                     DTANVEPMAQV"
     gene            4052..5491
                     /gene="gatA"
                     /locus_tag="VFR42_06270"
     CDS             4052..5491
                     /gene="gatA"
                     /locus_tag="VFR42_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU0368793.1"
                     /translation="MKRNLTIDGIKHALASKRLSARELTTDFFIKINARNPQINAYLE
                     LSPERAYAQADKIDALVAAGKPLPPLCGVPVAIKDVLSTRGITTTCGSKILRNYVPPY
                     DATAVARLEEAGAIILGKTNCDEFAMGSSNENSAYGAVKNPVAMDRVPGGSSGGSAAA
                     VAAGLAVASLGTDTGGSIRQPGAFCGIPAMMGSYGRVSRYGLIAFASSLDRIGPFANS
                     VHDVAAVLEAIAGRDPNDSTSTTAPVPAYTEEINKPIKGLRIGIPRDYFAEGMDSGVR
                     DKVEKGIELLKSLGCQPVDIRMPHTDYAIATYYIIATAEASSNLARYDGVRYGLRVDD
                     PTLIGMYRKTRGAGFGAEVKRRIVLGTYVLSAGYYDAYYLKGQKVRALIAQDFRDAFQ
                     KVDVIVTPTSPTPAFKLGEKTDDPLQMYLADIYTVTGSLAGVPGISVPCGKICGTLPV
                     GLQLFGPAFGESRILRLAHAFEQAGGASL"
BASE COUNT         1179 a         1981 c         1464 g         1047 t
ORIGIN      
        1 gcttcctcca cgcggccttt tttcgccagc cagcgcgggc tccgcggaat cccaaacagc
       61 atcaccagaa aaatcgctgc aggcagcgcc gcaatcccca gcttccatcg ccactccgca
      121 ccgcccagcc caatcgtccc aatcaaataa ttcgacaggt acgccagcaa aatcccaaat
      181 acgacattga actgaaacaa tcccactagc cggccgcgat atttcgccgg agcaatctcc
      241 gcaatgtaca tcggacccag caccgacgac cctccgatcg ccagcccgcc aatcacacgg
      301 aacaccacca gcgccgtcca gtcccacgcc agaccgcatc caatcgccga aatcaggtac
      361 aacaccgcca gaatccgcaa actgtcgcgc cgcccccact tctcgcccgg aatccccgct
      421 agcattgatc caacaatggt cccggccagc gcgctcgaca ccgtcacgcc cagcgatagc
      481 ggcgtcaggt gaaataactc cgtcaacgat agcgtcgtcc ccgaaatcac caccgtgtca
      541 aacccaaaca gcagcccgcc cagcgccgcc accaccgcgc tccgcaacaa aaccggattc
      601 aatcccatcg ctcacacctt cttccacttg accccaccgc cggctccgag ctccgaggtt
      661 ttcatgtagc gccgggcttt agcccggcat cttcgccgcg cctgctaccg tcgctccagc
      721 cgcgcgcatt ctactcaatt cctcttccgc agcacagcta ctctctcatt ggcacccacc
      781 cgcgccattg gagtatcttt ctcacgggac ccgggaacac ggagcacgca acaacatgcg
      841 cacccaagta gccatcatcg gcgccggccc ggccggcctc ttcctatccc tcctcctcga
      901 ccgcctcggc atccagtccg ccatcctcga actccacacc cgccagtaca tcgaagagcg
      961 ggtccgcgcc ggcctcctcg aacacaacac cgttcagctc atgcacgaac tcggcgtcgg
     1021 cgaccgcctc aagcgccagg gcctcgagca cggcggcatc gagctccgct tcaacaatcg
     1081 cggccaccgc atcgacttcc gcgaactcgc ctccggcaaa tgcgtcacca tctacgccca
     1141 gcacgaagtt atcaaggatc tcgttgcagc ccgcctcgct gccaacaagc ccatcctctt
     1201 cgaaacggcc aacctgcagc tccacgccgt cgactctaca agtccctacg tcaccttcca
     1261 gcacaacggc cagaaaagta tcgagcgcct cgactgtgac ttcatcgccg gctgcgacgg
     1321 cttccacgga gtctcccgca cctcaatccc gcaaggcctc ctcactacct atgaacgcga
     1381 ctaccccttc ggctggctgg gcatcctcgc ccaagccccg ccttcttccc acgagctcat
     1441 ctacgcacac cacgaccgcg gcttcgctct tctctctatg cgttctccgc aactcagccg
     1501 tctctatctg caatgcgtgc ccgacgaaaa tctcgcgctc tggcccgacg accgcatctg
     1561 gcgcgaactg caaaagcgcc tcgccactca cgacggctgg aaactcaccg aaggccccat
     1621 catccaaaaa ggtgtcaccg gcatgcgcag cttcgtggtt gagcctatgc agtacggtcg
     1681 cctctacctc gccggcgact ccgcccacat cgttcccccc accggcgcca aaggactcaa
     1741 cctcgccgta gccgacgtcc gcgtcctcgc cgtcgcgctc ggcgcctact actccaccgg
     1801 cgccgccgat ctcctcgccc aatactccgc cacttgcctt cgccgcgtct ggaaagtcca
     1861 gcgcttctcc tggtggatga ctcagctcct ccaccgcttc cccgacgaca atcccttcga
     1921 tcaccgccgc cagctcgccg acctcgacta cctcaccacc tcccgcgccg cctcccaaac
     1981 cctcgcggaa aactacgtcg gcttgccttt accctgacgg acgcgaaagc ggacggtagg
     2041 ccggcccatt ttggctcagc ctcggtgggt ttgaacgtaa cgcccggctt cagcctggca
     2101 tcctcgcgtc gtgccgtgat tccgccgctc gtaccgctcc ccgacgccgc cttctcccga
     2161 atggcgctaa acgacctgct cattttcatc cgggaatcgc cgatttttca ggggatcgga
     2221 gcatcagtta cactgagccc gaagggctcc ggcaacgccg cgccacaaaa aaaggggggc
     2281 ttccgacggc tatggcggcc caggacgctg agccgccgta gttttgttaa actaatggag
     2341 atgtcttccc ttacacaaat aacaggattc gacgctgccg caacgcggca cccgcggcgc
     2401 aaaacctacg ccgtaaaagt cggcgacgtt caagtaggcg gcggtgcgcc catcgttgta
     2461 cagtccatga ccaacacgga cacggccgat gtcgagtcca ccctcaagca gacgctggag
     2521 ctggccgaag caggttcgga gattgtgcgc tggacggtga atgtgccgga agccgccgcc
     2581 gcggttcccg aaatcaagca gcgcctgctc gccgccggct gcaatgttcc catcattggc
     2641 gactttcatt acaacgggca tgtgctgctg acgaaatatc cggaatgcgc caaggcgctc
     2701 gacaaatacc gcatcaatcc gggaaatgtg ggtgcgggcc agcgccgcga tgaacagttc
     2761 tccaccatct gtaaagtggc gcgtgataac ggcaagccca tccgcatcgg cgtcaacggc
     2821 ggctctttga atcaagagct ggtcatggcc aaaatgcagg agaacaccga caaaaatctc
     2881 ggcctcgaat ccggagagat catcaatcag tgcatggtgc tgtccgcggt gcagtccacc
     2941 gaactcgccc tcgaaactgg catgcgtcat gaccagatca ttatttcgtg caagacttcc
     3001 cggccccgcg atttgatcat ggtgtatcgc gatctggcga gcaaaaccga acaaccactg
     3061 catctgggct tgacggaagc cggcatgggc atgaaggggc tggtgtggtc ggcggcgtcg
     3121 ctgggtgtcc ttttgtatga cggaattggc gacaccatcc gcatttcact gacgccacgg
     3181 cccggcggcg accgccgcga cgaagtctac gccgcctgcg aactgcttca gtcgctaggc
     3241 ctgcgctctt tttcaccgag cgtcactgca tgccctggct gcggccgtac taccagctca
     3301 acgttccagg aattggcgga gcgcacgcag gactacatcc gcgaaatgat gcccagctgg
     3361 aagacgcagt acgaaggcgt cgaggctatg acgcttgccg tcatgggctg catcgtgaac
     3421 ggtcctggtg aatccaaagc agccaacatt ggcatttcgc tgcccggcac cggcgaagca
     3481 ccccgctgcc ccgtctacat cgacggcaag aaagacgtca ccctcgaagg cacctacgac
     3541 gaacttgccg ccgccttcca gcaactcgtc gacacctacg tctccacgaa atacccccgc
     3601 aagagtgcga gcgtatcctt atagaatttc tagaccatca gaagtctttc attatctggg
     3661 ctaaaccgtt tggcgcgaat cggcctgtgc taaaatcgcc cacatgaaaa tttcgcgcga
     3721 agacgtcgta cgggtggcgg agctggcgta tctcgacttg agcgaaagcg aactggaaac
     3781 ttaccgcgcg caggttgacg aaattctgga atacattggc aagttgaacg agctggatac
     3841 ggccaacgtc gaaccgatgg cccaagtgta gacagacgac cagacggctg acgcgacatt
     3901 gcgggatgac gttgtggtgc cctgcggtat cgccgctgaa gtcttgaagc aggcgcctga
     3961 cccggagccg cccttcttcc gagtgcccaa agtcatcgag cgctagcttt tcgttttgcg
     4021 gctctcgggc cgattcgcgc gcggattcgc aatgaagaga aaccttacga ttgacggcat
     4081 aaaacacgct ctggcgtcga agcggctcag cgcccgggag ctgaccaccg atttctttat
     4141 aaaaatcaac gcgcgcaatc cgcaaattaa cgcctatctc gagctctcgc ccgaacgcgc
     4201 ctacgcgcaa gccgacaaaa tcgacgcctt ggtggccgca gggaagccgt tgcctcctct
     4261 ctgcggcgtt cccgttgcga tcaaggatgt gctttccacc cgcggcatca ccaccacatg
     4321 cggctcaaaa atccttcgta actatgtccc gccctacgat gcgacggcag tcgctcgcct
     4381 cgaagaggct ggcgcgatca ttctcggcaa aaccaattgc gacgagtttg ccatgggcag
     4441 ctccaacgaa aactccgcct atggcgcggt gaagaatccg gtcgcaatgg accgcgtacc
     4501 cggcggctcc agcggaggtt cggcggcagc agtggcggca gggctcgccg tggcctctct
     4561 gggcaccgac accggcggct ccattcgcca gccaggagca ttttgtggaa tccccgcgat
     4621 gatgggcagc tacggtcgcg tctcgcgcta cggcctgatt gccttcgcgt catcgctcga
     4681 ccgcatcggc ccctttgcca actccgtgca cgacgtcgcc gcagttctcg aggctattgc
     4741 cggacgcgat cccaacgatt cgacttccac taccgcgccg gttccggcct acaccgagga
     4801 aataaacaag ccgatcaagg ggctgcgtat cggaatccct cgcgattatt tcgccgaagg
     4861 catggattcc ggcgttcgcg acaaagtgga aaaaggcatc gagctgctga aaagtctggg
     4921 ctgtcagccg gttgacatcc gcatgcccca caccgactac gccatcgcca cttactacat
     4981 catcgccacg gctgaagcca gttcgaacct ggcgcgttac gacggcgtgc gctacggcct
     5041 gcgtgtcgac gaccccactc ttattgggat gtaccgcaag acgcgagggg ccggctttgg
     5101 ggcggaagtt aaacgccgga tcgtcctggg cacctacgtt ttgtcggcgg gatactatga
     5161 cgcttattac ctgaagggcc agaaagtccg ggccttgatc gcgcaagact ttcgcgatgc
     5221 cttccagaag gtcgacgtca ttgttacacc gacgtcgccg acccccgcat tcaaactcgg
     5281 cgaaaaaacc gacgatccgc tgcaaatgta tctggccgac atctatacgg tgaccggatc
     5341 gctcgccggc gttcccggca tttccgttcc ctgcggcaaa atctgcggca cgctgcccgt
     5401 cggcctgcag ctctttggtc cggcctttgg cgagtcccgc attttgcgac tggctcacgc
     5461 gttcgaacaa gccggcggcg ccagcctgta agaccagcat ttgccagtct tattgcgtcg
     5521 cggcctccgc gtccaattcc ctgctaaact cgcagaattc agtgcagccg cagcctgtga
     5581 tccagaagct gttgtccagc gaggaacgtt cctgcgagcc gcggatggtg tcgtcgcacc
     5641 agattggccg gccttctatt acagacttgc a
//
LOCUS       DASTPM010000359         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_41567, whole genome shotgun sequence.
ACCESSION   DASTPM010000359 DASTPM010000000
VERSION     DASTPM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41567"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFR42_06275"
     CDS             <1..493
                     /locus_tag="VFR42_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0368794.1"
                     /translation="PGQGTTFKVYLPRFQEEAETNVAAKVRTTAPKGSETILLVEDDE
                     AVRELTEMVLKTYGYNVLVAEEPQHAQKLSDAPGMDIALVLTDVVMPTMSGRELVKRL
                     TDKHPHLRVLYMSGYTDNVITSGGVLEPGLAFLQKPFTPGLLANKVREVLDAPAAVGK
                     QIP"
     gene            complement(551..2095)
                     /locus_tag="VFR42_06280"
     CDS             complement(551..2095)
                     /locus_tag="VFR42_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368795.1"
                     /translation="MRITIRYCGLVALLCACSAADCLAQDPDKAIAQYVKAAGGSGKL
                     SKLHTVSLEGALTRVSDGQTGAFTLDTKSPNRYYLELLVGEDPEILSYNGKSAWRKSA
                     NGEAMTLLEQDALQLEAAAFLANSHLLDLHKNKVGVAYVGTAKLGSSDAQQIELTMPT
                     GVKRQLFFDASSHLLKKDSGAIGNAPFEVAYDDYRAEDGVLIPHKLQLARAGETYDIV
                     IHRVSVNGVIGERVFDFPHKSQIQLPDLRQLFAEIDANQKAIDKLKENYTGRRAVEET
                     QYDSSGKVTKLERREETFFYLDGEDISTLVAKDGKPLSDDEQRKENERVQKRIAKHQE
                     EKVHKEKKEEKAREEGKDDKDSDDPGIEIFLRACQFVNPRRERFRGQDVLVFDFEGNP
                     EYKPKKLAERVVQQLAGVVWVDEKAHDVVRLEAYFVKDVHFGGGLLANLQKGTSFVFE
                     QAFLNNEVWLPTYEEAHIGARVLLVKGFKVNEVTRYSDYHRFNIETLSTINKPKLAEN
                     PPPDKP"
     gene            2229..3656
                     /locus_tag="VFR42_06285"
     CDS             2229..3656
                     /locus_tag="VFR42_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0368796.1"
                     /translation="MEPSPASTAPYLKRVLGLRDLVLLGIAAVFNLNLVPPVAAGGFP
                     SLFLWGVGLLCFFLPQGVAVAEFAVRYPEEGGVYLWTKKTFGQLHGFLSGWFYWTTNV
                     FYIPTLLVYLIGIVSFEAGPHGEAWEPGVAWQVAFSLALLWLFTWASIRGLGVNKWMN
                     NLGGFAAIVTALILLAVGAKVLWQHQNMALPSAAGWLPALSGWRTLSALGVICLGLVG
                     LELGSTMGDEIHQPRRTVPRAVMIAGLASGFLYVAATFAVLLALPVQEIGVVTGVLQA
                     FASMGGKVGLSGMHLLVAIVLSIAICGALSAWLSGGGRIPFVAGVARYLPAMLSRVHG
                     KWQTPHVALIAQAVASSVVIGLSFAGKDVRLHEAYDVLLALAVITQMIPFVYLNAGLV
                     RVAGTPGGLYRGKLWLRLLGAVGLATTCVAIATAFIPPADIANVWRYEAKLVFGLALF
                     SFGGTGLFFRYASRKASKASEQQLA"
BASE COUNT          708 a          912 c         1295 g          892 t
ORIGIN      
        1 gccggggcaa gggacgacgt tcaaagtata tttgccgcgg tttcaggaag aagccgaaac
       61 gaatgtggcg gcaaaagtgc gaacgacggc tccgaagggc agcgagacga ttctgctggt
      121 ggaagacgat gaagcggtac gcgaactgac cgagatggtg ctgaagacgt atggctacaa
      181 cgtgctggtg gcggaagagc cgcagcacgc acagaaattg tcggatgctc cggggatgga
      241 cattgcgctg gtgctgacag acgtggtgat gccgacaatg agcgggcgag aattggtgaa
      301 gcgattgacg gacaagcatc cgcacctgcg cgtgttgtac atgtccggct acacagacaa
      361 cgtgattacc agcggtggag tgctggagcc ggggctggcg tttttgcaga agccgttcac
      421 accgggattg ctggccaaca aagttcggga ggtgttggat gcgccggcgg ctgtggggaa
      481 gcagattccg taagaccgca ggtatcaagt ataaagtgaa gagttccagg tctgacagca
      541 ggcggacacg ctagggcttg tcggggggcg gattttcggc caacttcggt ttgttgatcg
      601 tggaaagagt ttctatgttg aagcggtggt agtcggagta acgggtcact tcgttgacct
      661 tgaaaccctt aacaagaaga acgcgggctc cgatgtgagc ttcttcgtaa gtgggaagcc
      721 agacttcgtt attgagaaag gcttgttcga aaacgaagct ggtgcctttt tgcaggttgg
      781 cgagaaggcc gccgccgaag tggacatctt tgacgaagta agcttctagg cgcaccacgt
      841 cgtgcgcttt ttcatcgacc cacacgacgc ccgccaattg ctgcacgaca cgctcggcaa
      901 gctttttggg cttgtactcc gggttaccct cgaaatcgaa gacgagaaca tcctggccgc
      961 ggaagcgttc gcggcgcgga ttgacgaact ggcaggcgcg gagaaaaatc tcgatgcccg
     1021 gatcatcgga gtccttgtcg tccttacctt cttccctggc tttttcttcc ttcttttctt
     1081 tgtggacctt ttcttcctga tgtttggcga tgcgtttttg cacccgctca ttttccttgc
     1141 gctgctcgtc gtcgctgagc ggtttgccgt cctttgcgac aagtgtagaa atgtcttcgc
     1201 cgtcgagata gaagaaagtt tcctcgcggc gctcgagctt ggtgactttg ccggacgagt
     1261 cgtattgcgt ttcttccacg gcgcggcggc cggtgtagtt ttctttcagc ttgtcgatgg
     1321 ctttctggtt ggcgtcaatt tcggcgaaga gctgtctgag gtctggaagc tgaatttgcg
     1381 atttatgagg gaaatcgaag acgcgctcgc cgatcacacc gttgacggag acacgatgga
     1441 tgacgatgtc ataggtttcg cccgcgcgag caagctgcag cttgtggggg atcagcacgc
     1501 cgtcttcggc gcggtagtcg tcgtaagcga cctcgaacgg agcgttgccg atggcgccgg
     1561 aatctttttt caggaggtgc gaactggcat cgaaaaagag ctggcgtttg acgccggtgg
     1621 gcatggtcag ttcgatttgc tgggcgtcgg atgaaccgag cttagctgta ccgacatatg
     1681 cgacacctac tttattcttg tgaagatcga gcaagtggct gttggcaagg aaagcggcag
     1741 cttcgagctg cagggcgtct tgttcgagta gagtcatggc ttcaccgttg gcggacttgc
     1801 gccaggcgga tttcccattg taggagagga tttcgggatc ctcgccgaca aggagttcga
     1861 ggtaatagcg gttgggcgat ttggtgtcga gcgtgaaggc gccagtttgg ccatcggaaa
     1921 cgcgggtgag agcgccctcc aggctaacgg tgtggagttt ggagagtttt ccgcttccac
     1981 cggcggcttt gacgtattga gcgatggcct tatcgggatc ctgggcgagg caatcggcgg
     2041 cgctgcaagc gcagagcaag gcgacgaggc cacagtagcg gatggtgata cgcatggtgt
     2101 ctgcaactag aaacgcgaaa gcggcggaaa agtttcggtt ccccggtgaa ttgtttgact
     2161 tagcctcgcg gccagcctat tatcggcgcc cttctgcgca tagcgccagg gcgcgcgcag
     2221 tggcctacat ggagccgagc ccagccagca cagcacctta cttgaagcgc gtcttgggac
     2281 tgcgcgatct ggttctgctg ggcatcgcgg cggttttcaa cctgaatctg gtgccaccgg
     2341 tggcggcggg aggttttccc agcctgtttc tgtggggcgt gggcttgctg tgcttttttc
     2401 tgccgcaggg agtggcggtg gcggaattcg ccgtgcggta tccggaagaa ggcggcgtgt
     2461 atctgtggac caaaaaaacg tttggccagc tgcacggctt tttgagtgga tggttttact
     2521 ggacgacgaa cgttttttac attcctacat tgctggtata cctgatcgga atcgtctcgt
     2581 ttgaagcggg gccgcacggc gaagcgtggg agccaggtgt cgcgtggcag gtggcatttt
     2641 cgctggcgct gttgtggctg ttcacgtggg caagtatccg cgggctgggc gtaaataaat
     2701 ggatgaacaa cctgggcgga ttcgcggcga tcgttaccgc gctgattttg ttggctgtcg
     2761 gcgcgaaagt tttgtggcaa caccaaaaca tggcgctacc gagtgcggcg ggatggctgc
     2821 cggcgctttc cggatggcgg acgctgtcgg cgctgggcgt gatttgcctg gggctggtgg
     2881 gattggaact gggctcgacg atgggagacg agattcacca gccgcgccgc accgtgcccc
     2941 gcgcggtgat gattgcggga ctggcgagcg gattcttata tgtggcggcg acgtttgcgg
     3001 tgctactggc gctgccagtg caagaaatcg gcgtcgtgac cggagtgctg caagcgtttg
     3061 ccagcatggg cggaaaagtg ggcttgagcg ggatgcacct gctggtcgcg attgtactgt
     3121 cgattgcgat ttgcggcgcg ctctcggctt ggctgtcggg cggcgggcga attccatttg
     3181 tggcgggcgt ggctcgatat ttgccggcga tgttgtcgcg agtgcacggc aaatggcaaa
     3241 cgcctcacgt cgcgctgatt gcgcaggcgg tagcttcgtc agtggtgatc gggttgagct
     3301 tcgccggcaa ggatgtgcgg ctgcacgaag cgtacgacgt gttgctggcg ctggcggtaa
     3361 ttacgcaaat gattccattt gtgtatttga acgcggggct ggtgcgcgtg gccggcactc
     3421 cgggagggct gtaccggggc aaactgtggc tgcggcttct gggagcggtt ggactggcga
     3481 cgacgtgcgt ggcgatcgcg acggcgttca ttccgccggc ggacattgcg aacgtgtgga
     3541 gatacgaggc caagctggtg tttggcctgg cattgttttc atttggcgga accggattgt
     3601 ttttccgcta tgcgagccgc aaagcttcaa aggccagcga gcaacagctc gcgtagctca
     3661 ttctagaggc gcccgacctg cggtgcgtca aggcttaggg cggtttgaat atttttcgaa
     3721 caagcgtttg ttccggttgt ccgtggaagt gagctggcct ttaatgaaga gatatttcag
     3781 gtcggttgtc agctcaagcg gatcgcc
//
LOCUS       DASTPM010000360         5068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1108448, whole genome shotgun sequence.
ACCESSION   DASTPM010000360 DASTPM010000000
VERSION     DASTPM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108448"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..936
                     /locus_tag="VFR42_06290"
     CDS             94..936
                     /locus_tag="VFR42_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0368797.1"
                     /translation="MNGVIANTEEIRFTPYITASGILHGGLIVLIAVSAYFHWDGNRW
                     AGPGGGSENVTNVKLVGNSGMPLPTPPSLNESNVVDPSKSLWKNDVKPEPPQPKKEEI
                     VPPDLKNVPEFKTLEKNNKKPPPLKKENKAFEPKQPNPDNAVPGRNLGAMKVPTGAGT
                     QPGASSTPGMAIKAEGGGDFASRYGWYIEAVKRRIQTNWLQNTIDPGVLAAHSAHAVV
                     EFTIYKDGTVKDIRISQTSGNQSMDNSGMRAIMSSNPMPGLPPDYSGSTVRVIFDFDL
                     SMTR"
     gene            989..2341
                     /locus_tag="VFR42_06295"
     CDS             989..2341
                     /locus_tag="VFR42_06295"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368798.1"
                     /translation="MSLSALRRALFALFTLAVMFAGRPAMAQQQDWFRIGTGLGVEKP
                     RVAVADFTPRSDTAKSHGALFTQVVRDDLAFSGIMDMVSPSFYPQSQPSAPNELRFVS
                     WTDSPVNAHFVAFGNLSESTGEVAIEAWFYDVTNPSSQAVIAKVYRGTPTDAQVRKFA
                     HQFADEIIMRLSGGLPGLSSTQIAFVSSRGGAKEIWAMDYDGANQHVLTSLHSIALTP
                     RWSPDSARIAFTCYASGGGAPASPQICMYSMDANRVVSFPRYRGTNSAPAWSADGSQL
                     VFSSSMGGNPELYSISAGGSGLKRLTFSNGASTSPAWNPKTNQTIAFVSDRSGVPKLY
                     LMNSDGTNAQNLDLPDKGYLIDPAWSPNGQLLAFSWRRPSNNYDIYIMDVATRQIIEL
                     THDTGRNERPSWAPDGRHIVFESTRSGTRQIWTMLADGSQPHQLTNSGHNESPNWSAR
                     "
     gene            2474..3223
                     /gene="pal"
                     /locus_tag="VFR42_06300"
     CDS             2474..3223
                     /gene="pal"
                     /locus_tag="VFR42_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEU0368799.1"
                     /translation="MSEVARPFYRACLLTAFAAAALTVGCGKKPVVSRPEPTPPPPAA
                     RPTVTLQANPTTINKGDATTLSWSSTNATQLTISPEVGAVAPEGSTKVTPSESTTYTV
                     TASGPGGTADSTVRVTVGAAPPPPPPAQNATLEEMFLKEVQDAYFDLDKADIRSDARA
                     ALAKTADFLRNYPQVKVVVEGHCDERGSTEYNLALGDRRAAAVKQYLVSLGIGADRMS
                     TVSYGKEKPFCTESNEDCWQRNRRGHFVMAK"
     gene            3476..4399
                     /gene="ybgF"
                     /locus_tag="VFR42_06305"
     CDS             3476..4399
                     /gene="ybgF"
                     /locus_tag="VFR42_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU0368800.1"
                     /translation="MRFRNIAAFGVALVVGAVGGSLIGPRPARAVAREIIELQRDVTT
                     LLQGQKDLSTQLTQDHTVMRTLLEQSNDSVGRLNGTMSGLQKSVQDVQANSGARLDTM
                     STQVQGLSDNLEEIKSRLGKLNQQLVDLQNTVQSLDAKISGGVAPAGTVPTTSPTGAS
                     TTQPLGGGAASSASGPAPSADTLYSNGLRDITSGKYDLARSEFEDYLKYYGDTDLASN
                     AQFYLGEIAYSQRQYSQSVSEYDKVLTNYPKSFKLAPARLKKGMALLEMGQKSQAIKE
                     LREVVKRYPGTEEERRARAKLKELGVAATAA"
     gene            complement(4432..>5068)
                     /locus_tag="VFR42_06310"
     CDS             complement(4432..>5068)
                     /locus_tag="VFR42_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368801.1"
                     /translation="MAFAPPVVAYWLGQAGRAGFDSRPIRFSTRRQPRVRSFLSGVAI
                     LVLSGFAAVCPAAAQGPAGNGAAEAAGNGSSSHSYNPTKWFGKKDHKAAANVPVDELD
                     QKLEARLRAAQVLAPDASLRDVCNNFAERVDCIAALRASHNLGLNFKCVKASMTGVRT
                     DADASSCRMPTDDKPLNLTKTIRLLKSDADAKSAAKDAETAAREDVKNATA"
BASE COUNT         1094 a         1390 c         1596 g          988 t
ORIGIN      
        1 gtcggtcatc gacacgttgc ggcaatccgg catcaataat attagtatcg tgacgcagcc
       61 gctgacagag cgaaaccgcg gatcgtaaga attatgaacg gggtaatcgc caacacggag
      121 gaaatccgct ttacgccgta tataacggcg tctggcatat tgcatggcgg actgatcgtg
      181 ttgatcgcgg tttcggccta ttttcattgg gacggaaacc gctgggccgg gccgggcggc
      241 gggagcgaaa acgtcaccaa cgtgaagctg gtaggcaaca gcggaatgcc gctgcctacg
      301 cctccatcgc tgaacgaaag caacgtggtg gatccttcca agagcctctg gaaaaacgat
      361 gtcaagccgg agccgccgca accaaaaaag gaagagatcg ttccgccgga tttgaagaac
      421 gttcccgaat tcaaaacgct ggaaaagaac aacaagaagc cgcctcccct aaaaaaagaa
      481 aacaaggcgt ttgaaccgaa gcagccgaat ccggacaatg cagtgccggg acgcaatctt
      541 ggtgcaatga aagtgccgac cggcgccggt acgcagcccg gggcatcgag tacgccgggc
      601 atggcgatca aagccgaggg cggcggggac tttgcttcgc gctacggctg gtacatcgaa
      661 gcggtgaagc gccggattca gaccaattgg ctgcagaata cgattgatcc cggcgtgctt
      721 gcggcacaca gtgcgcatgc ggtggtggag ttcacaatct acaaagatgg aacggtaaaa
      781 gacatccgca tttcgcaaac cagcggaaac cagtccatgg acaattccgg catgcgagca
      841 attatgtctt ccaatccgat gccggggttg ccgcccgact attcgggctc gaccgtgaga
      901 gtgattttcg attttgacct tagtatgaca agataaatgg cgaaattccc ttcccagccg
      961 ccagagatca agttccatgg aggagctagt gtctttgtcc gcgttgcgta gagctttgtt
     1021 cgcactgttt acgttggcag tgatgtttgc cggacggccg gccatggcgc agcagcagga
     1081 ctggtttcga attggcacgg gactaggcgt ggaaaagccg cgggtggcgg tggcggattt
     1141 tacgccgcga tcggataccg cgaagtcgca cggcgctttg tttacgcaag tggtgcgcga
     1201 cgacctggcg ttcagcggga tcatggacat ggtgagcccg agcttttatc cgcagtcgca
     1261 gccgagcgcg ccgaatgagc tgcgcttcgt ttcctggacg gactcgccgg taaatgcgca
     1321 ttttgtggcg ttcgggaatt tatcggagag cacgggcgaa gtcgctattg aggcctggtt
     1381 ttatgatgtg acgaacccgt cgtcgcaggc ggtgatcgcc aaggtgtacc gcgggacccc
     1441 gacggacgcg caagtgcgca aatttgcgca ccagttcgcg gatgagatca tcatgcggct
     1501 gtcgggaggg ctgcccgggt tgtcttcgac gcagatcgcc tttgtaagct cgcgcggcgg
     1561 ggccaaggaa atttgggcga tggattatga cggcgcgaac cagcacgtgc tgacttccct
     1621 gcactcgatc gcactgacgc cgcgatggtc gccggacagt gcgcgcatcg cgtttacctg
     1681 ctatgcgtcg gggggcggag cgccagccag tccgcagatc tgcatgtact cgatggatgc
     1741 gaatcgcgtg gtgagttttc cacgctatcg cggcaccaac agcgcgccgg cgtggtcggc
     1801 ggacggttcg caactggtat tttcgtcttc gatgggcggg aatccggagc tgtattccat
     1861 cagcgcaggc ggtagcggat tgaagcggct gacgttttct aacggggcga gcacttcgcc
     1921 agcctggaat cccaagacga atcagacgat cgcgtttgtg agcgaccggt ccggcgtgcc
     1981 gaagctgtac ctgatgaatt ctgacggaac gaatgcgcag aacctggatt taccggataa
     2041 agggtatttg atcgatccgg cttggtcgcc gaacgggcag cttctggcgt tcagctggcg
     2101 gcggccttcg aacaactacg acatttacat aatggatgtg gcgacgcggc aaattatcga
     2161 actgacgcac gacacggggc ggaacgaacg gccgagctgg gcgccggacg gtcggcacat
     2221 cgtgtttgag tccacgcgtt cggggacgcg gcagatctgg accatgctgg cagacgggtc
     2281 acagccgcac cagctgacca acagcggaca caacgaatcg ccgaactggt cggcgcgctg
     2341 attcgatttt gtacggttcg tttcaggaaa tatatcgtag tgcgatgttt tatgggttcc
     2401 gttgtggtgg taccatgtgg tggctcgaaa tcgtaagagt tcatggggat gcacactgag
     2461 gaggaaatct agaatgtctg aggttgcccg accgttttac cgcgcttgtc tcttgactgc
     2521 atttgccgcc gccgccctca ccgtgggatg cgggaagaaa cctgttgtat ccaggccgga
     2581 acctacacca ccgccgcccg cggctcggcc aaccgtgaca ttgcaagcaa atcccacgac
     2641 catcaacaag ggtgacgcga cgaccttgag ctggtcttcg accaatgcga cgcagctgac
     2701 gatttctccg gaagtaggcg cagtggcgcc ggaaggttcg acgaaagtga ctccgtcgga
     2761 gtccaccacg tatacggtta ctgccagcgg accgggcggc acggcggact ccaccgttcg
     2821 cgtgacggtt ggcgcggcac ctccacctcc gccaccggcg caaaatgcga cgctcgaaga
     2881 aatgtttctg aaggaagtgc aggacgccta ctttgatctg gataaggcgg acattcgcag
     2941 cgatgcgcgg gcagctttgg ccaagaccgc ggactttctt cgcaactacc cgcaagtgaa
     3001 agtggtggtt gagggccatt gcgacgaacg tggttccacc gaatataacc tggcgctggg
     3061 cgaccggcgt gccgccgcgg tgaagcaata tctggtttcc ctgggcatcg gagcggaccg
     3121 catgagcacg gtcagctacg gcaaagagaa gcccttctgc acggaaagca acgaagattg
     3181 ctggcagcgg aaccgccgcg gccacttcgt gatggccaag taggccgaac gactcagcgg
     3241 cttaggccag accgcaactt aggctgcaac caggcgccgc actacgggtt tctccttata
     3301 ccgggcggcg ccgctgttct ggacactggt gttgtgatgg aaggaaggcg acccggccgc
     3361 gcgggaagca gcctcttgct accttacggc tctctcgcta cagtgaagtg agaagcttca
     3421 cgcgccgaac ctgggcgaag tgcgtctggc gcgccactga ggaggagctt tatccatgcg
     3481 ttttcgtaat attgcggcgt ttggagttgc cctggtagtc ggggcggtgg gcggaagcct
     3541 gatcggaccg cgtccggcac gtgccgtggc tcgcgaaatt atcgagctgc aacgcgacgt
     3601 cactaccctg ctgcaggggc agaaagacct ttccacgcaa ctgacgcagg atcacacggt
     3661 gatgcggacg ttgctggagc agtcgaacga ttcggtcggc agactgaacg ggacgatgag
     3721 cgggctgcag aagtccgtgc aggacgtgca ggccaattcg ggagcacggc tggacaccat
     3781 gtccacgcag gtgcaggggc tttcggacaa tctggaagag atcaagtcgc ggctcggaaa
     3841 gctgaatcag cagctggtag atttgcagaa cacggtgcaa agtctggacg caaagatttc
     3901 cggcggcgta gcaccagccg gaactgtgcc caccacaagc ccgacgggag cgtcgactac
     3961 tcaaccgctg ggcggcggag cggccagcag cgccagcgga ccagctccca gcgcggacac
     4021 gctttattcg aacggattgc gcgacatcac cagcgggaaa tacgatctgg cgcgctcgga
     4081 gtttgaagac tacctgaagt attacggcga tacggatttg gcatcgaatg cgcagtttta
     4141 tctgggcgag attgcgtatt cgcagcggca gtattcgcag tcggttagcg aatacgacaa
     4201 agtgctgacc aactatccga agagtttcaa gctggctccg gcacgtttaa agaaaggcat
     4261 ggcgctgctg gagatgggac agaaaagcca ggccatcaag gaacttcggg aagtcgtgaa
     4321 gcgatatccc ggcacggaag aagagcggcg tgcgcgggcg aagttgaagg aactgggcgt
     4381 agccgcgacg gctgcgtagc tcccggtttt tcgctcctta acctgccagc ttcaagccgt
     4441 tgcgttcttc acatcctcac gcgcagcagt ttctgcgtcc ttcgccgcgc tcttggcgtc
     4501 agcatcagac ttcagcaagc gaatcgtttt ggtcaggttt agtggtttgt cgtccgtagg
     4561 catgcggcag gagctggcgt cggcgtccgt acgaactccc gtcatgctgg ccttaacaca
     4621 cttaaaattc aaaccaaggt tgtgactggc gcgcaaagct gcaatgcagt cgacgcgctc
     4681 cgcaaaattg ttgcagacgt ctctcagcga ggcgtccggc gccaagactt gtgcggcgcg
     4741 aagtcttgcc tctagctttt ggtccagttc gtcgaccggc acgttcgcag cggccttgtg
     4801 gtccttcttg ccgaaccatt ttgtaggatt gtaggaatgc gaacttgagc cgttgccggc
     4861 ggcttccgcg gcaccattgc cagccggccc ttgcgccgcg gcgggacata cagcagcgaa
     4921 ccccgacagc accaagattg ccacaccgct tagaaatgat ctcaccctgg gttgcctcct
     4981 cgtggaaaag cgaatgggcc ggctatcgaa tccggcgcga ccagcctgac ctaaccagta
     5041 cgccacgacg ggcggggcga atgccatg
//
LOCUS       DASTPM010000361         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_70213, whole genome shotgun sequence.
ACCESSION   DASTPM010000361 DASTPM010000000
VERSION     DASTPM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70213"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VFR42_06315"
     CDS             <1..751
                     /locus_tag="VFR42_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU0368802.1"
                     /translation="WMVDRTEQLVKKCREWYAAYEFHRVYHAIHDYCVVDLSAFYYDV
                     LKDRLYTKAPNNPARRSAQTAIYKITSALVRIMAPILVFTAEEIWKYLPKPANHAASV
                     HVALFPDESELRSGIAAAQAAHWDLLAKVRGEVLKALETARNEKKINSGLEAKVLLNG
                     NPELKAKLKAHLAQLPGLFIVSQVDFFSAGAPDYKSEVVPSLVVCVHKAEGAKCERCW
                     NYSTHVGENSRYPTVCERCSAALAEIENGNL"
     gene            782..1285
                     /gene="lspA"
                     /locus_tag="VFR42_06320"
     CDS             782..1285
                     /gene="lspA"
                     /locus_tag="VFR42_06320"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU0368803.1"
                     /translation="MIHPSRLRWLILTLVVVFLDRLSKAAIEAKTVEGWRHELVHNFI
                     YLVHSKNTGIAFSIFADSASPWTRYSLIGGSLVVIAILAWYLVAGSAISPRAAAGLAL
                     LLGGATGNLTDRILRGGVTDFFEVWFGSYRYPAFNVADSAITIGAVLILLDVLFPRRA
                     FTKSSVK"
     gene            complement(1302..>2214)
                     /locus_tag="VFR42_06325"
     CDS             complement(1302..>2214)
                     /locus_tag="VFR42_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0368804.1"
                     /translation="VAIFRLSLVLCALFTSELTKAQEVAITFDDLPAHGPLPAGVTRE
                     EVTRKILAAFKEAKVPEVYGFINAGKLQAHPEDIKVLRLWREAGEPLANHTYEHMSLN
                     RNPLAAFRENVAKDQATLQELMGNNDWHWFRYPYLDEGDTLEKRRAARAYLKEHHYRI
                     AQVSLDFEDYAWNGPYARCSEKKDEKVMAWLKESYLNTATEYMKLGQQLSQQLFGRNI
                     KHILLMHVGAFDAEMLPQLLAQMKQQGFKFITLKEAESDPVYEIDPDIVRKEGSPILE
                     QIMDARHLPLPPHAEKPMKELQEVCRP"
BASE COUNT          444 a          723 c          561 g          486 t
ORIGIN      
        1 ctggatggtc gatcgcaccg agcaactcgt caaaaaatgc cgcgagtggt acgccgcata
       61 cgaatttcac cgtgtctacc acgccattca cgactattgc gtagtggacc tgagcgcgtt
      121 ctactatgac gtcctgaaag accgcctgta taccaaagct cccaacaatc ccgcgcgccg
      181 ttctgcacag actgccatct acaagatcac cagcgcgctg gtgcgcatca tggcgcccat
      241 tctggtcttc accgccgaag aaatttggaa atatctgcct aagccggcga accatgccgc
      301 gagcgtccac gtcgctctgt ttcccgacga atccgaacta cgatcgggca ttgccgcggc
      361 gcaagccgcc cattgggatc tgctcgccaa agtgcgcggc gaagtcctga aagcgctcga
      421 aaccgcacgc aacgagaaga aaatcaactc cgggctggaa gccaaagtcc tgctgaacgg
      481 caaccccgag ctgaaggcca aactcaaagc tcacctcgcg caattgcccg gcctattcat
      541 cgtgtcgcag gtggacttct tcagtgccgg cgcgcccgac tataaatccg aagtggtacc
      601 gtcgctggta gtctgcgtac acaaggccga aggcgccaag tgcgagcgct gctggaacta
      661 ctccacgcat gtcggtgaaa actcgcgtta cccaacggtc tgcgagcgct gttcggccgc
      721 gttggcggaa atcgaaaatg ggaatttgta accgggttcg caaagtcgct cgagttcgat
      781 catgatccat ccgtcccggc tccgctggct gattctgacc ctggtggtcg tttttctcga
      841 ccgcctttcc aaagccgcca tcgaggccaa gaccgtcgaa ggctggcgcc acgaactggt
      901 ccacaacttc atctatctgg tgcacagcaa aaacaccgga atcgctttca gcatctttgc
      961 cgacagtgcg tcgccgtgga cgcgatacag tctgatcggc ggctcgctgg tcgtgatcgc
     1021 cattctcgcc tggtatctgg tggccggctc cgcaatttcg ccccgcgcag ctgcgggcct
     1081 cgcactgctc ctgggcggag ccaccggcaa cctcaccgac cgtattctgc gcggcggtgt
     1141 aaccgatttc ttcgaagtgt ggtttggttc ctaccgctat cccgcgttca atgtcgccga
     1201 ctcggcaatt accatcggtg ccgttctgat cctgctggac gtgctcttcc cgcgacgcgc
     1261 cttcacgaag agttccgtaa aataacgcga agtccgttcc cctacggccg gcaaacctct
     1321 tgcagttcct tcatcggctt ttctgcatgc ggcggcaacg gcaagtgcct ggcgtccata
     1381 atctgttcga gaatcggcga cccttccttc cgcacgatat ccggatcaat ttcgtacacc
     1441 ggatcggact ctgcctcctt cagcgttatg aacttgaacc cctgctgctt catttgcgcc
     1501 agcagttgcg gcagcatctc cgcatcaaac gcgcctacat gcatcagcaa gatgtgcttg
     1561 atgttgcggc caaaaagttg ttgcgaaagt tgttgtccca gcttcatgta ctccgtggct
     1621 gtgttcagat agctctcctt cagccacgcc atgacttttt catccttctt ttccgaacac
     1681 cgcgcgtacg gtccgttcca cgcgtagtct tcaaagtcca gcgaaacctg tgcgatgcgg
     1741 tagtgatgct ccttcagata agcacgcgcc gcccgccgct tctccagcgt gtcgccctca
     1801 tccagatacg gatagcgaaa ccagtgccag tcgttgttcc ccatcagctc ttgcaacgtc
     1861 gcttggtctt tcgcgacgtt ctccctaaac gccgccaacg gattgcggtt caggctcatg
     1921 tgctcgtagg tgtggttcgc cagcggctct cccgcttcgc gccacaaccg cagcaccttg
     1981 atatcctccg gatgcgcctg caatttcccg gcgttgataa acccgtagac ctcaggcacc
     2041 ttcgcttctt taaacgccgc caaaatcttc cgcgtcacct cttcccgcgt aacccccgcc
     2101 ggcagcggcc catgcgctgg cagatcatca aacgtaatcg ccacttcctg cgccttcgtg
     2161 agctcggatg tgaacagagc gcacagaacc agcgacagcc ggaaaatcgc aacg
//
LOCUS       DASTPM010000362         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1425789, whole genome shotgun sequence.
ACCESSION   DASTPM010000362 DASTPM010000000
VERSION     DASTPM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425789"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VFR42_06330"
     CDS             complement(<1..243)
                     /locus_tag="VFR42_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0368805.1"
                     /translation="MSTRPIPTKDAASDPKAAKIDPKAAPVDMKFEIVVLPVADVNRA
                     KDFYAKLGWRLDADYAHENDFRVIQFTPPGSGCSVIF"
     gene            complement(627..1829)
                     /locus_tag="VFR42_06335"
     CDS             complement(627..1829)
                     /locus_tag="VFR42_06335"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEU0368806.1"
                     /translation="MQTFTTGCFLLAALLFPLLALVQPSTALDNGLALTPPMGWNSWN
                     KFHCNVSEDLIKSMADGMVASGMKDAGYQYVVIDDCWQVSRDENGNIVPDPQHFPSGM
                     KVLGDYIHSKGLKFGIYSDAGEKTCAGRPGSMGHEFQDALMYAKWGVDYLKYDWCHTG
                     KRNAEEAYATMRDALKAAGRPILFSMCEWGTAKPWLWAAGTGNLWRTTGDIDDHWEGK
                     KKWPGGGCCSLGMLDILDLQVGLESFAGPGHWNDPDMLEVGNGGMTTSEYRAHFSLWC
                     IINAPLIAGNDLRSMAPEIKEILTNKEVIAINQDPLGRQASRAWKEGNLEIWAKPMKD
                     GSRAVLLLNRGASEAEITAPWNVLGYPDRLSASVRDLWSHKDLGKFTAKFSVKVPSHD
                     VVVVTVKR"
BASE COUNT          403 a          526 c          709 g          464 t
ORIGIN      
        1 gaaaatgacg gagcagccag agcccggcgg cgtgaactgg atgacgcgga agtcgttttc
       61 gtgggcgtag tcggcgtcga gccgccagcc gagctttgcg tagaagtcct tcgcgcggtt
      121 cacgtccgca acgggcagga cgacgatctc gaacttcatg tcgactggtg cggctttcgg
      181 gtcaatcttc gcggccttcg ggtcgcttgc ggcgtctttc gttgggatcg gcctggtgct
      241 catggtgctc ctttgctagc gtggattggg cgtgctgggc tgagtttcgc ggcttcggcg
      301 gaaagagata gtacttcctg tttgaaggct tggcgatagg attcctgtcg gatggcgttt
      361 tccacaggtt gggttctcct tttttttcgt aagagccgca aataaaggac ttacaacgcc
      421 caaagtaaaa agagtgaaga agggcaccgg aacgaacccg ttctggatgc gcaccccggt
      481 gtgaagattc agaaggtcaa aacagctcag ctttagcgag aataacgagc ggcgatgagt
      541 cggctaccgc ggtcacgcct ttgagacgtt caaccgttcg gctttcatgc gccaaagcgc
      601 ccgccttaaa aggcggccgc tacatgctaa cgtttaaccg tgactacgac gacgtcgtgg
      661 gaggggacct tcacggagaa ttttgcggtg aatttgccta ggtctttgtg cgaccagagg
      721 tcgcggacgg aggcggagag gcggtcggga tagccgagga cgttccacgg ggcggtgatt
      781 tcggcttcgc tggcgccgcg atttaggagg aggacggcgc ggctgccatc tttcatgggc
      841 ttggcccaga tttcgaggtt tccttccttc cacgcccggc tggcttggcg gcctagcgga
      901 tcctgattga tggcgatgac ttccttgttg gtgaggattt ccttgatttc gggggccatg
      961 ctgcgcagat cgttgccggc aataagcggc gcgttgatga tgcaccacag gctgaagtgg
     1021 gcgcggtatt cgctggtggt catgccgccg ttgccgactt cgagcatgtc gggatcgttc
     1081 cagtggccgg ggccggcgaa ggattcgagg ccgacctgga gatcgagaat gtccagcatg
     1141 ccgagcgaac agcagccgcc accgggccac ttctttttgc cctcccagtg gtcgtcaatg
     1201 tcgccggtgg tgcgccacag gttgccggtg ccggcggccc agagccaggg cttggcggtg
     1261 ccccattcgc acatgctgaa gaggatggga cggccggcgg ctttgagcgc atcgcgcatg
     1321 gtggcgtagg cttcttcggc gttgcgcttg ccggtgtggc accagtcgta tttgagatag
     1381 tcgacgcccc atttggcgta catcagagcg tcctgaaatt cgtggcccat gctgccgggt
     1441 cggccggcac aggttttctc tcccgcgtca gagtagatgc cgaacttgag gcctttggag
     1501 tggatgtaat cgccgaggac tttcatgccg gagggaaagt gctgcgggtc gggaacgatg
     1561 ttgccgtttt cgtcgcggga aacctgccag caatcgtcaa tgacgacgta ttggtagccg
     1621 gcatctttca tgccgctggc gaccatgcca tcggccatgg acttgatgag gtcttcgctg
     1681 acgttgcagt ggaatttgtt ccaggagttc cagcccatgg gaggagtgag ggcgaggccg
     1741 ttgtcgagag cggtggaggg ctggacgagg gcgagtagcg ggaaaagaag agccgcaagg
     1801 agaaagcagc cggtcgtaaa cgtttgcatt cagggcctcc tgccgcagtg gtccgtttga
     1861 attttgtcct tatggtgtcg cgatccaaaa attcaaaccg accgacgcgg aaaaatccgg
     1921 agagatgata caggaaaaag ggatggtggc gtgcatttag gaagagcgcg aaagggcgga
     1981 cggttggtct cggaaattta tcgcggcgct gaattatttt tctttgaggc cgcagaaagc
     2041 gggtgcaggg cgcttcgcaa ttgcaaaacc cagccgtata ccggtcgcag gaagggatta
     2101 aa
//
LOCUS       DASTPM010000363         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_185884, whole genome shotgun sequence.
ACCESSION   DASTPM010000363 DASTPM010000000
VERSION     DASTPM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185884"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..735
                     /locus_tag="VFR42_06340"
     CDS             370..735
                     /locus_tag="VFR42_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368807.1"
                     /translation="MDTGCMARYSAFLGRAVEVLYRAGDICLPASGTFVADSGRSIFL
                     EQHYEQCGQQKNFRWEIPYQCIVRIEETEISSAPSLASAATEVLGTPAAAAGPLQANA
                     AHASAGPSLLPLTRRPETA"
     gene            859..1740
                     /locus_tag="VFR42_06345"
     CDS             859..1740
                     /locus_tag="VFR42_06345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0368808.1"
                     /translation="MPQPTVTLIGLGLMGRPMGHNLLKAGFPLVVWNRTASRADSLVA
                     AGARLAPSPKEAAAAADVLITIVSDPAALEQVLWGTDEKSGGAFAAIKKGAIYLDSST
                     VSPALARKIAEASSSAGIRFLDAPVTGGTWGAEKGELLFMTGGDAQTLEAARPVISAM
                     GKKIFHLGPNGAGQTIKLAMNLLLALQVDAFAEALALVTGSGLQGEKLVEVMQSSMAR
                     SGVLDVKAPVMLKADYTPSFPLRLMHKDLSLALDLGNQIGVPLPAAAAAREIYNAVKG
                     GSKDDLDYAAVYKFWTR"
     gene            complement(1741..2103)
                     /locus_tag="VFR42_06350"
     CDS             complement(1741..2103)
                     /locus_tag="VFR42_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0368809.1"
                     /translation="MMDVILKRFEEPDEVRVFEKGRFELVHVGGMTIGRACYEPGWKW
                     SVHVGEATGARSCLVEHVGMVISGKATAAMDDGRVIEMKAGDVFYIGPGHDSWVLGEE
                     PYVSLHFLGADNYASAKK"
     gene            complement(2123..>2682)
                     /locus_tag="VFR42_06355"
     CDS             complement(2123..>2682)
                     /locus_tag="VFR42_06355"
                     /inference="COORDINATES: protein motif:HMM:NF036579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0368810.1"
                     /translation="LGIWLDVIPRDRRKNALAKVLSKSDAGFAASGEVPAMTVATYYF
                     RFYLARAVDYAGLGDDYLKLLGPWREMVSLGLTTWAESPEPTRSDSHAWSAHPNYDFL
                     RIVAGVRPGSAGFEKITIEPHLGALKNVKAAMPVPQGVVEVEYQRSADGLEARVNLPD
                     GAHGEFLWKGRVTVLHAGTQTVRLP"
BASE COUNT          518 a          867 c          692 g          605 t
ORIGIN      
        1 gggtgacctt cgcaaagtag cggcgcatgc tagttgcaag ttatttgcaa acatgctgag
       61 aattcttccc ttgttgcaag caggtgtcaa ctagactgtg ctgaacggcc ttttttgcgg
      121 gtccaacggc taatatacga cccgaatagt cctatatgct accaccgtgg ttggagccgg
      181 cactcccgcg cgaacaaggc cagggtgatt gtgtggctcg ttaaaacctc cgctgttgct
      241 tcccgtgcct ggcggcagca cgcttacggc ttttctgcag tccagtgaaa aactgtttcc
      301 agttgaaacg attttccaca aaattttccc cataaaatcc ttcgtactat tgaccatgga
      361 actgtccgtt tggacactgg ctgcatggct cgttattcgg cctttctagg ccgtgccgtg
      421 gaagtcctat atcgcgctgg agacatttgc cttcccgcct cgggaacatt tgtggccgat
      481 tccggccgct ccatatttct cgaacagcat tacgaacaat gcggccaaca aaagaatttc
      541 cgctgggaga ttccctacca gtgcattgtt cgaattgagg agacggaaat ctcttcggct
      601 ccttcactgg cttccgcggc gactgaggtc ctcggcactc cggcggccgc agccgggcct
      661 ttgcaagcga atgcggcgca cgcttccgcg ggcccttctc ttcttccgtt gacacgacgg
      721 cccgaaaccg cctgacgttg tgatccattt cccgcgttga gcgttcgtat cacgccaact
      781 gttttgttct gttcgctgca ctacgcgtta caatcttccc gttccgttca tcgaactttc
      841 ttgtcattgg agagagtctt gccacaacct accgtcactc tgattggcct cggtctcatg
      901 ggccgtccca tgggacacaa ccttctcaag gccggatttc cgctcgtcgt atggaaccgt
      961 accgcctctc gcgcggattc gctggttgct gccggcgccc gtctggcccc ctcaccgaaa
     1021 gaagctgctg ccgctgccga cgtcctcatc actatcgtga gcgatccggc cgcgcttgag
     1081 caggtcttgt ggggaaccga cgaaaaatcc ggcggcgctt ttgccgccat caagaaaggc
     1141 gcgatctatc tcgactccag cacggtttcg ccagcgctgg cgcggaagat tgccgaggca
     1201 tcgtcttccg cgggtattcg cttcctcgac gcaccggtca ctggcggcac ctggggcgcc
     1261 gaaaagggag agttgctgtt catgactggt ggcgacgcgc agacgctaga agccgcgcgg
     1321 cccgtgattt ccgcgatggg caagaaaatc ttccatctcg ggccaaacgg agcgggccag
     1381 acaatcaagc tcgccatgaa cttgcttctt gcccttcagg tggacgcgtt cgccgaagcg
     1441 ctcgcgctcg tcaccggctc cggcttgcaa ggcgaaaaac ttgtcgaagt catgcaatcc
     1501 agcatggcgc gctccggtgt tcttgacgtc aaagcgccgg tcatgctgaa ggctgactac
     1561 acgcccagct tcccgttgcg cctgatgcac aaggacctca gcctcgcgct ggatctcggc
     1621 aatcagatcg gcgtgccgct gcccgctgcg gccgctgctc gcgagatcta caacgctgtt
     1681 aaaggcgggt cgaaagacga tctcgactac gcggctgtct acaaattctg gacgcgctga
     1741 ttatttcttc gcgctcgcgt agttgtcggc tcccagaaaa tggagcgaca cgtaaggctc
     1801 ttcgcccaaa acccagctgt cgtgccctgg cccgatgtaa aagacatcac ccgctttcat
     1861 ctcaatcacc cgcccatcgt ccatcgcagc agtggctttg cctgaaatca ccatcccgac
     1921 atgttcgaca aggcaactcc tggcgcctgt cgcctccccc acatgcaccg accatttcca
     1981 tccgggctcg tagcacgcgc ggccaatcgt cattccaccg acatgcacga gttcgaagcg
     2041 gcctttctcg aaaactcgca cttcgtccgg ctcttcaaat ctttttagaa tcacgtccat
     2101 catcagcgtc gtttctcctt cactagggta ggcgtaccgt ttgcgtgccc gcatgcaaga
     2161 ctgtgacgcg acctttccac aagaactcgc cgtgtgcgcc gtccggcaag ttcacccgcg
     2221 cctccaagcc gtctgcgctt cgctggtact ctacttccac cacaccttgc ggcaccggca
     2281 tcgccgcctt cacgtttttc agcgcgccca gatgcggctc gatcgttatc ttctcaaagc
     2341 ctgcgctgcc tggccggact cccgcgacaa ttcgcaagaa atcgtagttg ggatgggcac
     2401 tccacgcatg cgaatcagag cgcgtgggtt ccggcgattc ggcccaggtc gttagtccca
     2461 acgaaaccat ctcccgccac ggacccagca gcttcaagta atcatcgccc agcccggcat
     2521 agtccacagc ccgggccaag taaaaccgaa aataataggt tgccacggtc attgccggca
     2581 cttcgccgct tgccgcgaat cctgcgtcgc tcttcgacaa gacctttgcc agcgcgttct
     2641 tccggcggtc gcgcggaatc acgtcgagcc aaatccccaa aa
//
LOCUS       DASTPM010000364         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1079327, whole genome shotgun sequence.
ACCESSION   DASTPM010000364 DASTPM010000000
VERSION     DASTPM010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079327"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..695)
                     /locus_tag="VFR42_06360"
     CDS             complement(30..695)
                     /locus_tag="VFR42_06360"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0368811.1"
                     /translation="MRITQGILVLVGLASLPVSARADYLVLRNGARMNVSSYELRGDR
                     YRVQIDGGWAEIAAANVVAIEPEEIFVAAPKMPLTQAPFGELIRKSAEKYGVDVDLVF
                     SVVAAESNFNPKAVSRRNARGLMQLLPETAKRLGVRDIYDPAQNIDGGTRYLRDLLKL
                     YEGDLALALAAYNAGPGAVQRYGRIPPYNETIAYVRAIRKTYALRKSGNDKSSPSATA
                     TGH"
     gene            complement(686..1537)
                     /locus_tag="VFR42_06365"
     CDS             complement(686..1537)
                     /locus_tag="VFR42_06365"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU0368812.1"
                     /translation="MGVRLIALDIDGTLLDSRWKLPEANRAAICEAARRGMEVALVTG
                     RRYDFALPVAQNLGCPLTMIVNNGGMIRSSRGETLLRHLLSADVARHVLASCSRWREG
                     AAVIFDRPRADQLMLERLDADDSIRYAYYSRNLEFIGLAQPLESCLTEDPLQVMFSGT
                     IAEIEEAEQVLQKSSVKDRVRLAATKYAARDFAMLDVLPPGCSKGAALAEWAQRQGLH
                     RDEILAIGDNHNDLEMLHFAGIPVVMGNSVPELKTFGWHETHSNDESGVAAAIERFAL
                     REAQSCA"
     gene            1635..>2767
                     /locus_tag="VFR42_06370"
     CDS             1635..>2767
                     /locus_tag="VFR42_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU0368813.1"
                     /translation="MTTRRLYYDDAFQDNFTAEVLSCKPLADPLAGQDATCWGVLLDQ
                     THLYPTSGGQPNDLGKLGEANVLDVRDLENDDILHVVDRPVPAGRIDGCIDWPRRFDH
                     MQQHTGQHLLSAVFQERFGLPTVSFHLGESACTIDLRGPQPSVQILEGAARAANAIVF
                     EDREISVRYGTAEQFAQMGVRKQVERTGTLRAIEIAGIDLQPCGGTHVRRTGQIGTIL
                     LRGCSKIRQDWRIEFLCGSRAESAARKDADMLARVAAQLKCAPQDLDGSVERLLQERE
                     VATRNLKNLLPKMADAVVATRLQSIAPSGGGEVVVVEMLQNLGPEYLQFYATALTRSE
                     KVVALLAVQETGALLFAQHPTVGKDMNALLRGVFDQLGGKGGGT"
BASE COUNT          547 a          890 c          754 g          576 t
ORIGIN      
        1 tctaattcca attttccagt ttcgaggctt tagtggccag tggccgttgc cgagggcgag
       61 gacttgtcgt tgccagactt ccgcagggca taggtcttcc gtatcgcgcg cacatatgcg
      121 atcgtttcgt tgtagggagg aatcctccca taccgctgaa cggcgccggg tcctgcgttg
      181 tacgccgcca gcgccagcgc caggtcgccc tcgtaaagtt tcagcaagtc gcgcagatag
      241 cgcgtgccgc catcgatgtt ttgcgccgga tcgtaaatgt cccgtacgcc caatcgtttc
      301 gcggtttccg gtagcagttg catcaagccg cgagcattac gccgcgaaac cgctttgggg
      361 ttgaagttcg attccgcggc tacaacactg aacaccaaat ccacgtccac accgtatttt
      421 tccgccgact tgcggatcag ctcaccaaat ggcgcctgcg taaggggcat ctttggcgcc
      481 gccacaaaaa tctcttccgg ctcgatggcc accacgttcg cagcggcaat ctcggcccag
      541 ccgccgtcaa tctgcacccg gtacctgtcg ccgcgcagtt cgtaagagct gacattcatc
      601 cgcgctccgt ttcgcagcac cagataatcc gcgcgcgcgg atacgggaag cgacgccaag
      661 cccactagaa cgaggatgcc ttgcgttatg cgcacgactg agcctcgcgc agcgcgaacc
      721 gctcgatcgc cgccgctaca ccgctttcat cgttggaatg ggtctcgtgc cagccaaacg
      781 ttttcagttc cggcacgctg ttccccatca ccaccggtat gcctgcaaag tgcagcattt
      841 caagatcgtt gtggttgtcg ccaatagcca ggatttcgtc gcggtgcagc ccctgccgct
      901 gcgcccactc cgccagcgcc gctcctttgg agcagcccgg aggtagtaca tccaacatcg
      961 caaaatctcg cgcggcgtac ttggttgcgg ccaggcgcac tcggtccttg accgaagatt
     1021 tctgcaaaac ttgctcggcc tcttctattt ccgcgatggt gccggaaaac atcacttgca
     1081 gcggatcttc ggttaagcac gactctagcg gctgcgccaa gcctatgaat tccagatttc
     1141 gggaataata cgcgtagcgg atcgagtcgt ccgcgtcgag ccgctccagc atcaactgat
     1201 cggcgcgtgg ccggtcgaaa atcaccgccg cgccctcgcg ccagcgtgag cacgaggcca
     1261 atacgtggcg cgcaacgtcc gcggacagca aatgcctcag cagggtttcg ccgcggctgg
     1321 agcgaatcat tccgccgttg ttcacaatca tggtcagagg acagcccaaa ttctgcgcta
     1381 ctggcaatgc gaagtcatac cgtcgaccgg tcaccagggc cacttccatc ccgcgccgcg
     1441 ccgcttcgca aatcgccgct cggttggcct ccggcagttt ccagcggctg tccagcagcg
     1501 tgccgtcaat gtccagcgcg atcaggcgga ctcccatata ggctatttta cctgcatttg
     1561 acggccgaaa acggcacgta tcctactcgt gttgcgaaaa acacgcaaat tgcgtgtgct
     1621 agactcgctt tgttatgacc actcggcgcc tctattacga cgacgcgttt caggacaatt
     1681 tcacggctga ggttcttagt tgtaagcctc ttgccgaccc gcttgctggg caagacgcca
     1741 cctgctgggg cgttttactc gatcagactc atctttaccc cacttccggc ggtcaaccca
     1801 acgaccttgg taagctcggt gaagccaatg tcctcgatgt tcgcgacctg gagaacgacg
     1861 atatcttaca cgtggtggac cgtccggtac ccgctggacg cattgatggc tgcatcgact
     1921 ggcctcgccg cttcgatcac atgcagcagc acaccggcca gcacctcctc tccgcagttt
     1981 ttcaggaacg cttcgggctg cccaccgtct cctttcacct tggtgaatcg gcttgcacca
     2041 ttgacctccg cggcccgcaa ccgtcggtgc aaattctgga aggcgcagca cgcgccgcca
     2101 acgccatcgt ctttgaagac cgcgaaatca gcgtacgcta cggcacggct gagcaattcg
     2161 cacaaatggg tgtccgcaaa caagttgagc ggacgggaac gctgcgcgcc atcgaaattg
     2221 ccggcataga cttgcagcca tgcggcggca cgcacgttcg ccgcaccggt cagatcggca
     2281 ccatcctgct gcgcgggtgt agcaaaatcc gccaggattg gcgcatcgag tttctctgcg
     2341 gcagccgtgc cgaatccgcc gcccgcaaag atgccgacat gctcgctcgc gtcgctgccc
     2401 aactcaaatg tgccccgcaa gacctcgacg gttccgtcga acgtctgctg caggagcggg
     2461 aggtggccac caggaattta aaaaaccttc ttcccaaaat ggcagacgcc gtcgtcgcaa
     2521 cacgcctgca atcgatcgca ccatcaggcg gcggcgaagt ggttgtcgtg gaaatgttac
     2581 aaaaccttgg cccggaatat ctccagtttt atgccaccgc tctcacgcgc tccgaaaagg
     2641 ttgtcgcgct cctggccgtt caagagaccg gcgcgcttct gtttgcacag caccctactg
     2701 ttggtaagga catgaacgcc ttgctgcgcg gcgtctttga tcagttgggc ggaaaaggtg
     2761 gaggtac
//
LOCUS       DASTPM010000365         5956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_589467, whole genome shotgun sequence.
ACCESSION   DASTPM010000365 DASTPM010000000
VERSION     DASTPM010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5956
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589467"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2771)
                     /locus_tag="VFR42_06375"
     CDS             complement(114..2771)
                     /locus_tag="VFR42_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368814.1"
                     /translation="MSFLRNITSGLRALVRKKRADAELDEELRGYLEMAAEEKMKRGM
                     SRRESLRAVRLEEGSAEASREEVAAAGWESFVETCWQDIRFGARTLKKSPGFTAVAVL
                     TLALGIGANTAIFSYIDAWLIKPLPYPEPERLMVFESHDRKNGWTAANITSTASFLDY
                     QKQNTSFEQTAGWASWAFNLTGDGEPALVDGARVSWNFFDALGAKPLLGKTFTPDEDQ
                     AGTGHVAILAEGLWQSRYGGDPNVIGRTITIEGEPYAVVGVMPGKFQFTLRGLCNLWT
                     PLALTDKQRTDRGSSFFSAFGRLKAGVTREQAEAESKTIFATLEKQFPQTNTNLTQLV
                     ASMKEEIARNEGAPQVLMCLAIVGLVLLIACANVANLMLGRATNRATEFGLRGALGAT
                     RVRLVRQLLTESLLLFLLGGCAGALFGVFGVRWIEAQIPAHIRGYLVNYGHVNLSITT
                     LGFTLGIVVVCGIAFGLVPAFANSRLDANRTLKEASGQASGAKRGAALRRMFVAAEIS
                     LAVVVLISTTLLVRSFVISVRSSPGYNAANVMIAQVALPKTKYREEARQRNFSEDVLG
                     RLRALPQLEAAGAASSVPYGGFGAWVEAEAVGKPAPPPGERQGASYTAVSDDYFATMQ
                     IPLLKGRVFGSEDAPGNASAAIINQTMARALWPDEDPIGRQLRFGEQHAVCTVVGVVR
                     DIKMYYARERARPQMYVSLAQFPSATLGFVARIRGENATTATAVRDAIWAVDRNQPIS
                     SVEQLENIMGIVNSGDRTITRLMVFFGATAMFLAMMGIYGVMSNLVAQRTHELGIRAA
                     LGASPQQMMGMVMGQGLYLALAGIGIGLCCALLAGRALESMLYQVSPSDPATFATVPV
                     LFAAVALAACWMPARRAMAVNPVVALRCE"
     gene            complement(2776..5439)
                     /locus_tag="VFR42_06380"
     CDS             complement(2776..5439)
                     /locus_tag="VFR42_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368815.1"
                     /translation="MGWLRSMASGLRSLFRKERVERELDEELRAYLEMAVEERTQGGM
                     SRNEALRAVRLEQGGLDSAKEVVGAAGWESFVEALWQDLRFGVRTLRKTPGFTAVAIL
                     TLAIGIGANSAIFSVVNTVLLRPMPYKQADRLVTIWGENKPRGIDFDFASSLDYQDWR
                     SQSHSFESMGAATDVMYTLTGAGEPAGVIAYEFSPDFFDVLGVPAQLGRTFAHDEDQR
                     GKDQVAVLSHRLWMTRFGGDPTAVGRNITLDGKAYTIVGVMPPSFQYPSRTELWTPLT
                     APPDYVKDRGLRWFRVMARLKPGVTLERAQMEMKTIAARLQHEYPGTNKDQDVRLVSL
                     RQLISGDARPALLVLLCSVGLVLLIACSNLANLLLSRAVRRRREIAVRAALGASRLRI
                     VRQLLTESMLLSIAGGAAGVAIAYWGAHWLVRMFPTTISNLSIPRIERIPIDGWVLGF
                     ALLASVVTGILFGLAPALQACQIGSNQSLKEPARSGAVGATGRRFRNIVVVLEMATSL
                     MLLAAAGLMAKSFLRIVTADLGFRTDHVLTFRTLLPQHKYAKAWQRNDFHDDALRRIQ
                     SLPGVKAAGTVTFLPLSGWWGARAVSVAGRQTQPGQKTPSPVWSSVSPDYFQLLRIPL
                     LKGREFTNGDNASSAEVAILSAGLARSLWPDDDPIGRRVLIEGFEKPREVIGVVGDVH
                     QLGIAHPGENSDPTSEVYVPFAQAPGPLLGFVVRTEGDPLSIANAAQREIFAVDEEQP
                     VSFVESMDQLASETVALQRASTILLAVFAAMALALAAMGIYGVLSYSASQKTQEIGIR
                     IALGAVRADVLRIVMVEGVRLTVLGMTIGVAGALGFARLLSSLLYQVGAADQTIFLTV
                     PILLACVALAACYVPARRAARVDPMVALRCE"
     gene            complement(5446..5778)
                     /locus_tag="VFR42_06385"
     CDS             complement(5446..5778)
                     /locus_tag="VFR42_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0368816.1"
                     /translation="MAEKSSDLVQGTLDMLILKTLALEPMHGYGISVRIEQMSKGIFK
                     VNAGSLFVALQRLQRDGLIRGEWKSTENSRRAKYYVLTEQGRKRLNSETREWGRQVAA
                     IARILEAS"
BASE COUNT         1184 a         2035 c         1519 g         1218 t
ORIGIN      
        1 ccagccactg gcaagcttac gcagactggc gaaaccctga gcacgccttc gcccatctgc
       61 atctcgtttc ttccgctgga ttgattgtct gtcgcaggca ataatcggaa ggcctactcg
      121 caccgtagtg ccacaaccgg attcaccgcc atcgcccgcc gcgccggcat ccagcaggcc
      181 gccaacgcca ccgccgcgaa cagaaccggc accgtggcaa acgtcgccgg atcgcttggt
      241 gagacctgat acagcatgct ttccagcgcc ctgcctgcca aaagcgcaca gcaaagtccg
      301 atcccgattc cggccaaagc caaatacaat ccctgcccca tcaccattcc catcatctgc
      361 tgcggactcg ctcccagcgc cgcccgtatc ccgagctcgt gtgtgcgctg cgcaaccagg
      421 ttcgacatca ccccataaat tcccatcatt gccagaaaca tcgccgtcgc gccgaagaac
      481 accatcaacc gggtgatcgt ccggtcgccg gagtttacga tacccatgat gttctccagc
      541 tgttccaccg aagagatcgg ctgattgcgg tccacggccc aaattgcgtc gcgaaccgcc
      601 gtcgccgtgg tcgcgttctc ccctcttatg cgtgccacga atcctaaagt cgccgaagga
      661 aactgcgcca gggacacgta catctgcggc cgcgcacgct cgcgcgcgta gtacatcttg
      721 atatcgcgga ccacgcccac caccgtgcaa accgcatgct gttcgccgaa tcgcagttgc
      781 cgcccgatcg gatcctcgtc cggccaaagc gcgcgcgcca tggtctggtt gatgattgcc
      841 gcagaagcgt tgcctggcgc gtcctcggaa ccgaacacgc gccccttcaa cagcgggatc
      901 tgcatggtcg caaaatagtc gtcggaaacg gcggtataac ttgcgccctg ccgttctccc
      961 ggcggtggtg ctggcttccc aacggcttct gcctccaccc acgctccaaa tccgccataa
     1021 ggaaccgaac tcgccgcgcc ggccgcctca agttgcggca aggcgcgcag ccgccccagc
     1081 acgtcctcgc tgaaattccg ttgccgcgcc tcttcccggt acttcgtttt gggcagcgcc
     1141 acttgcgcga tcattacgtt cgccgcgttg tatccagggc tgctgcgaac ggaaataaca
     1201 aagctgcgca ccagcagcgt cgtcgagatc agcaccacca ctgccagcga aatctccgcc
     1261 gcgacaaaca tacgccgcaa agccgcgccg cgctttgcgc ccgaagcttg cccggacgct
     1321 tctttcagtg tccggttggc atccagccgc gaattggcaa acgcgggaac cagcccgaac
     1381 gcaattccgc acaccaccac aatcccgagc gtgaatccca gggtcgtaat gctcaggttc
     1441 acgtggccgt aatttacaag gtacccgcgt atgtgcgccg gaatctgcgc ttcgatccag
     1501 cgcaccccaa agactccaaa gagcgctcca gcgcagccgc ccagcagaaa cagcagcagc
     1561 gattccgtca gtagctgccg caccagtcgc actcgcgttg cgcctaacgc cccgcgcagg
     1621 ccaaactctg tggcgcgatt cgtcgcgcgc cccagcatca ggttcgccac gttcgcgcac
     1681 gcaatcagca gtaccagccc gacgatcgcc aggcacatca gcacttgcgg agcgccttcg
     1741 ttccgcgcaa tctcttcctt cattgaggca accagctgcg tcaggttggt gttggtctgc
     1801 ggaaactgct tttccagcgt cgcaaaaatc gtcttgctct cggcttcagc ctgttcgcgt
     1861 gtcaccccgg ctttcagtcg tccaaacgcg gagaaaaacg aactgccacg gtccgtacgc
     1921 tgcttgtccg tcagcgccag cggcgtccag agattacaaa gtcccctcag cgtaaattga
     1981 aacttccccg gcattactcc cactactgcg tacggctcgc cttcaatggt gatcgtccgc
     2041 ccgatgacgt ttgggtcgcc gccataccgg ctttgccaca agccctctgc gagaatggcc
     2101 acgtggcccg ttcctgcctg atcctcgtcc ggcgtgaacg tctttccgag cagaggtttt
     2161 gctcccagcg catcgaaaaa gttccagctt actcgcgcgc cgtcgaccag agcgggctcg
     2221 ccgtccccag tcagattaaa tgcccatgat gcccatccgg cggtctgctc gaatgacgtg
     2281 ttctgcttct gataatcgag aaacgaagcc gtggatgtga tattcgccgc ggtccagccg
     2341 tttttcctgt cgtgcgattc gaacaccatc aaccgctctg gctctggata cggcagcggc
     2401 ttgatgagcc aggcgtcgat gtagctgaaa atcgccgtgt tcgctccgat ccccagcgcc
     2461 agcgtcaata ccgcgactgc cgtaaacccc ggggacttct tcaatgtgcg agctccgaag
     2521 cgtatgtcct gccagcatgt ctccacaaac gattcccagc ccgctgcagc gacttcttct
     2581 ctcgaggcct cagcgctgcc ctcctccagc cgcactgcac gaagtgactc cctgcgactc
     2641 atcccccgct tcatcttttc ctcggccgcc atttccaggt acccgcgcag ctcttcatcc
     2701 agctccgcgt cggctcgctt cttccggacc agcgcccgca atccgctcgt aatgtttcgt
     2761 agaaaagaca tggttttact cgcagcgcag agcgaccatc ggatccactc gcgcggcccg
     2821 ccttgcgggc acatagcatg ccgcaagcgc gacacaagcg agcagaattg gcacggtcag
     2881 gaagatcgtt tgatccgcgg cgccaacctg gtacagcagg ctgctcagca accgagcgaa
     2941 gccgagcgct cctgccactc caatcgtcat tcccaatacg gtcaaacgca ctccttctac
     3001 catcacaatt cgcagtacat ccgcgcgcac cgctcccaac gcgattcgga ttccgatctc
     3061 ctgcgtcttc tgactcgcgg aatacgacag cacgccatag atccccatcg ctgccagcgc
     3121 cagagccatc gccgcaaaga ccgccaggag gatcgtgctc gcgcgttgca gggcgaccgt
     3181 ttccgaggcc agctggtcca tgctctcgac gaatgaaacc ggctgttcct cgtcaaccgc
     3241 gaaaatctcc cgctgtgctg cattcgcaat gctcagcgga tcaccctcgg tacgaacaac
     3301 gaagcccagc agcggtccgg gcgcttgggc aaaagggaca tatacttccg aagtcggatc
     3361 ggagttttcg ccgggatgtg caattccaag ctgatgcaca tcgccaacga cgccgatcac
     3421 ttccctcggc ttctcgaatc cttctatcaa gactcgtctt ccaatgggat cgtcgtcggg
     3481 ccacaagcta cgcgccaagc cggcgctcag aatggctact tctgcgctgg acgcgttgtc
     3541 cccgttcgta aattccctgc ctttcagcag cggaatgcgt agcaattgga agtagtccgg
     3601 cgaaaccgag ctccagaccg ggctcggcgt cttctgcccg ggctgagtct gacgtccggc
     3661 cacggacacc gcgcgcgcgc cccaccatcc gctgagcgga aggaatgtaa ccgttccggc
     3721 ggctttcacc ccgggcagcg actgaatccg ccttaacgcg tcgtcgtgaa agtcgtttcg
     3781 ttgccacgcc ttcgcatact tgtgttgcgg caaaagcgtg cgaaacgtca gcacatggtc
     3841 tgttctaaac ccaagatcgg ccgttacaat gcgcagaaag ctcttggcca tcaggccggc
     3901 cgcagccagc agcatcagtg acgtcgccat ttccagaacc acaacaatgt tccggaaccg
     3961 ccgcccagtt gcacccactg ctccgctgcg tgccggttct tttagcgatt ggttgctgcc
     4021 gatttggcat gcttgcaaag ctggcgcaag gccaaacaag attcccgtga ccaccgaagc
     4081 gagcagggcg aaacctagca cccagccgtc gatcggaatc ctctcgattc gtggaatgct
     4141 gagatttgaa atagtggtgg gaaacattct tacgagccaa tgcgcgcccc aatacgcaat
     4201 tgcaactccc gccgcgccgc cagcaatgct gaggagcatg ctttcggtca gcagttgacg
     4261 cacgattctc agccggcttg caccgagcgc tgcccgcaca gcaatttctc ttcgccttct
     4321 tactgcccgt gacagcagga ggttcgccag attcgagcac gcaatcagca gaaccagacc
     4381 cacggagcac aacaacacta acaaagcagg ccgcgcgtcg cccgatatca gctggcgaag
     4441 cgatacaagt ctcacgtctt gatccttgtt ggtacccggg tactcatgct gtaagcgtgc
     4501 ggcgatcgtt ttcatttcca tctgggctcg ctctagcgtc acgccgggct tgagccgcgc
     4561 cattacgcgg aaccagcgaa ggccgcggtc tttcacataa tccggcggag ccgtcagcgg
     4621 ggtccaaagt tctgtgcgcg acgggtattg aaaacttggc ggcataacgc caacgatcgt
     4681 gtacgctttc ccgtcgagcg taatgttcct gccaactgcg gttggatccc cgccgaaccg
     4741 cgtcatccac aatcggtggc tcagtacggc aacctggtcc ttccccctct gatcctcgtc
     4801 gtgtgcaaac gtccggccca gctgcgccgg gactcctaac acatcaaaaa agtccggcga
     4861 aaactcatac gcgatcaccc cagccggttc tccagcaccg gtcagtgtgt acatgacgtc
     4921 ggtcgcggcg cccatgctct cgaaggaatg actctgcgat ctccaatctt ggtaatccaa
     4981 ggatgacgcg aagtcaaaat cgattcccct tggcttgttc tcaccccata ttgtgaccaa
     5041 ccggtccgcc tgcttgtagg gcataggtcg aagcagaacg gtgttcacca cgctgaaaat
     5101 tgcactgttg gctccgatgc ctatcgcgag ggtaaggatt gccacggccg taaaaccggg
     5161 ggttttgcgc agcgtgcgga cgccgaagcg caagtcctgc cataatgcct ccacaaacga
     5221 ctcccagccc gccgcgccaa ctacctcctt cgccgaatcc agtcctccct gttccagcct
     5281 caccgctcgc agcgcctcat tgcggctcat gcccccctgc gttctttcct cgaccgccat
     5341 ctccaaatac gctcgcagct cttcgtccag ctcccgctca acccgttcct ttcggaatag
     5401 tgatcgaagc ccactagcca tgctgcgcag ccaacccatg cgttcttacg atgcctccag
     5461 aattctggcg atcgcagcga cttgtcttcc ccactcacgc gtttcgctgt taagcctctt
     5521 ccgcccttgc tccgtcaaaa cgtagtattt cgcccgccgg ctgttttccg ttgacttcca
     5581 ttccccccgg atcagtccat cgcgctgcag ccgctgcagt gccacaaaca aggagcccgc
     5641 attcactttg aaaatgcctt tgctcatctg ttcgatccgg acggagatcc cgtagccatg
     5701 cattggctcc agcgccagcg tcttcagaat gagcatgtcc agagtgcctt gtaccaggtc
     5761 gctggatttt tccgccatgg agcgcgccac ctcttgagcg tctagaggaa gaagaatatc
     5821 tttcctactg attgtcaaga ggaatgttgt gccgtcggta gtggccggtc tttagaccgg
     5881 ttcctttctt cgcttcgcga cactgcgttg accccgctcg tcgggcgcct tggggttatt
     5941 tcttttttcc gttttt
//
LOCUS       DASTPM010000366         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_906777, whole genome shotgun sequence.
ACCESSION   DASTPM010000366 DASTPM010000000
VERSION     DASTPM010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906777"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /gene="queG"
                     /locus_tag="VFR42_06390"
     CDS             <1..800
                     /gene="queG"
                     /locus_tag="VFR42_06390"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU0368817.1"
                     /translation="IAEMKMQFAQAEPFEALAYADTGPVHERVAAKYAGLGWLGKNTL
                     LINQQAGSWVFLGVILTSLRLIPTLGPEQLPAPDRCGTCRACLDACPTEAIVEPYVLD
                     ATRCISYLTIEYRGSITEHLRGQMRHHVFGCDICQDVCPWNRKSPRTESPEFQPRKFA
                     TRFENAAAENSLFRPELLMLANLSELDFREKFRGSPIKRAKWRGLVRNACIALGNSGL
                     TAESSAYPEALKSLRKLSESPDAVIAESARWALSRIQPEGNHSGGGD"
     gene            1023..1634
                     /locus_tag="VFR42_06395"
     CDS             1023..1634
                     /locus_tag="VFR42_06395"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU0368818.1"
                     /translation="MAFSVPPLPYAFDALEPYIDAKTMEIHHDKHHGAYVANLNKALE
                     GHADLQAKSIDDLLKNLDAVPENIRPAVRNNGGGHWNHTLFWTQMKKGGGGEPKGDLG
                     TAINSSFGSFAGFQEKFAAAGLGRFGSGWAWLVVKDGKLAIDSTPNQDTPYLLGGKAV
                     LGLDVWEHAYYLKYQNVRGDYIKAWWNVVNWDKAAELYAAAKK"
     gene            complement(1723..2334)
                     /locus_tag="VFR42_06400"
     CDS             complement(1723..2334)
                     /locus_tag="VFR42_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0368819.1"
                     /translation="MIRRSAGVHSMHPTQPAIAREKFFFYEKNTGVSHFHVESDEQGR
                     FPVEQAAGLLAMHCMVRNQTPADYVVMVQADSSNLAGLTEKAQKLLDAGRSVKSHVKL
                     TRREEEVLNGVMRSQANKEIAATLNLSERTVKFHVSSLLAKFRVRGRMELVREAAQHT
                     TPNFPLHEPVATRESRPLVSHVMNYRRGGGAFMSLPGREAVAQ"
     gene            complement(2804..4000)
                     /locus_tag="VFR42_06405"
     CDS             complement(2804..4000)
                     /locus_tag="VFR42_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0368820.1"
                     /translation="MNSLFIFLALAQILVGLYLVYEAIRWLGYVRRRAATDPGFYSPR
                     TAVLCPCKGVEPGLERNLTALCEFDHQNYEVFFALASESDSAAAIVKRVVAGSRGKAH
                     LIFAGAPRECGEKINNLRATIEQLPADFEVLVFADSDGRPGRSWLRKLTGPLNDSRIG
                     ATTTMRWLIANNNEFASLLLAAWNAPIVTMLGENTAKNFCWGGGTAIKRSVFDQSAVL
                     DEWQHSVSDDYSMTNALQRRSLPIVFLAECLTASYVETDFAGLMEFTHRQILITRVYS
                     PKMWLTAGLTHLLFCVTILLGIFTTLKSLLGGHPSMQFAALTFLPLLLAAIRGAIRVT
                     AAQEMLQSLKSQVQQQAWIHLVLGVWVPYLYSINFICSTFTRTIRWRGIRYELISPQQ
                     TKIVTS"
     gene            4118..4462
                     /locus_tag="VFR42_06410"
     CDS             4118..4462
                     /locus_tag="VFR42_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEU0368821.1"
                     /translation="MPGRVDSSMVTTALELPGYRIVRNYGVVRGIIVRSRSVIGNLGA
                     ALQTIVGGNITILTDLCERTRIEAYELLLMHAAEHGANAVVGFRYDATEVMQGVTEVL
                     AYGTAVQVEPHS"
     gene            complement(4471..>5031)
                     /locus_tag="VFR42_06415"
     CDS             complement(4471..>5031)
                     /locus_tag="VFR42_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368822.1"
                     /translation="AGEAPALLAQNLVQTLARERVVPRFVDSYVIENGRHALQVHAPL
                     YRDLLTILQREALLAACVRALEISTFETIENRRGKQKVVMRKGAESFRRKFLSALARQ
                     QNWNAGDALDFQSDLKMYEDLLARAASSRRPRKPYEAANHPFVDRCAFMLDSDFLEKA
                     RLAASRALASVEEIAAKLATEAVENA"
BASE COUNT         1135 a         1350 c         1507 g         1039 t
ORIGIN      
        1 ttatcgcgga aatgaaaatg caattcgcgc aagcggagcc attcgaagcg ctcgcctatg
       61 cggacacggg gccagtccac gaacgagtgg cggccaagta cgcaggactc ggctggctgg
      121 gcaaaaatac gctgctaatt aaccaacaag cgggctcctg ggtgttcctt ggtgtgattc
      181 ttacgagcct gcgcttgata ccgacgctcg gcccagaaca attgccggct cctgaccggt
      241 gcggaacgtg ccgggcgtgc cttgacgcgt gccccacgga ggcgattgtc gaaccctacg
      301 tgctggatgc cacaaggtgc atttcctatc tgacgatcga gtaccgcggg tcgattacgg
      361 aacatctgcg ggggcaaatg aggcaccatg tattcggctg cgacatttgc caggacgttt
      421 gcccgtggaa tcgcaaatcg ccgcgcaccg agtcgccgga atttcagccg cgaaaattcg
      481 ctacgcgctt tgagaacgct gctgccgaga actccctctt tcgacccgag ctgttgatgc
      541 tggcgaatct aagcgaactg gacttccgcg agaaatttcg gggcagccct atcaagaggg
      601 ctaagtggag aggattggtg cgcaacgctt gcattgcgct gggtaattca ggattgacgg
      661 cggagagctc cgcatacccg gaagccttga aatcgctgag gaaattgtct gaatctccgg
      721 acgcggttat cgccgaatct gcccgctggg cgctttcgcg catccaaccg gaaggcaatc
      781 actcgggcgg cggcgactag tcggcctgcg agttgagtgt cagaacggcg cggcatataa
      841 tccgcggtgt acacccatcg gcccaccgcg ctgaggagtt cctcgtgaaa cgcagggaat
      901 ttctgttcgc cggtagtatt gccggtgccg cgttctttcg accggaccac gccgctgccg
      961 gcttgctggc tgccggcggc cctgcaaagt acgaggctaa tctcacattg gaggacaaac
     1021 ctgtggcatt ttctgtgccc cccttgccgt atgcatttga cgctctggaa ccgtacatcg
     1081 acgcgaagac gatggaaatt caccatgaca aacatcatgg cgcctacgtc gccaacctga
     1141 acaaagcttt agagggccac gccgacttgc aagccaagtc catcgacgat ttgctgaaaa
     1201 atctggatgc ggtgccggaa aatattcgcc ccgcggtccg caacaacggc ggcggccact
     1261 ggaatcacac gttgttctgg acgcagatga aaaagggcgg aggcggtgag ccaaaaggcg
     1321 atctgggtac cgcgatcaat tccagttttg gatcgtttgc tggattccag gaaaaatttg
     1381 cggcggcggg cctcggtcgc ttcggctccg gctgggcttg gctggtggtg aaggatggca
     1441 agctggcgat cgattccacg ccgaatcagg acacgccata tttgctgggc gggaaagccg
     1501 ttctgggact ggatgtttgg gagcacgcct actacctgaa gtatcagaat gtgcgcgggg
     1561 actatatcaa ggcctggtgg aacgtggtga attgggacaa ggcggcagaa ctgtacgcgg
     1621 cagcgaagaa ataaccaaaa agctcaagct acaattgaaa agcccgtggt tcatcgccca
     1681 cgggcttttt ttattgcgac tgctggatga ttggcttggg aactactggg ccacagcctc
     1741 cctgcccggc aaggacataa aagcaccgcc gccgcggcga tagttcatta cgtgggaaac
     1801 cagcggccgc gattcgcgag ttgccaccgg ttcatgcagt gggaaattcg gagtagtgtg
     1861 ctgggccgct tcgcgaacca attccatccg gccgcgtacg cggaacttcg ccagcaacga
     1921 cgagacgtgg aattttaccg tacgttcgga aagattaagc gttgcggcaa tttccttatt
     1981 ggcttggctg cgcatcacgc cgttgagtac ttcttcttcg cggcgagtga gcttgacgtg
     2041 gctttttacg gatcggcccg catccagaag cttttgcgct ttttcggtca ggcctgccaa
     2101 gttggaagag tcggcttgga ccatgaccac gtagtcggcc ggagtttggt tgcgcaccat
     2161 acagtgcatg gctaacaggc cagcggcttg ttcgaccgga aagcgtccct gttcatctga
     2221 ctctacgtgg aaatgggaaa ctccggtgtt tttttcgtag aaaaagaact tctcccttgc
     2281 gatcgcaggc tgggtagggt gcatggaatg cacccctgca cttctgcgaa tcactctcac
     2341 tgaacctctc cgttcgcatt tctacccttg ggcagaaatg cctcgacgaa tataaatgca
     2401 cctcggagcg aggctaacgt ttacatccag attcaaattg acagagaacc agcacccgaa
     2461 accggacaag agggcgatgt ccaggcaacc gagcttcaag aacggtgaca ccgaggcgcg
     2521 agaaggagct ggtgtaaaaa ggcccgcctg cgtgcactac cgaactgcgg gtagcggtgc
     2581 acatacaagg ccaaacgggg cgtaccagaa ctgccgaaaa atggtagaga tatgcacact
     2641 tttgcctaca gggtttactg aatgcagggt aggaattgca cggtagcgga aaaatttgca
     2701 ttagtcaact gggagaattg acagtaggtc agggagcgga gtttgaagaa gctgcgcacc
     2761 aataaagcac tctatgtcct gtggaaactt tcacttacgt ccatcaggac gttacgattt
     2821 ttgtctgctg aggtgaaatc agctcatatc gaatgccgcg ccaacgaatg gtgcgggtga
     2881 atgttgagca aataaagttg atgctgtaga ggtacggaac ccagacgccg agcaccaggt
     2941 gaatccacgc ttgttgttgc acctgagatt tcagggactg cagcatttct tgcgcagcgg
     3001 ttacgcgaat tgcgccgcgg atcgccgcca ataaaagagg caggaatgtc agcgccgcaa
     3061 actgcatcga cggatgcccc ccgagcaggc ttttcagggt tgtaaatatc cccagcaaga
     3121 tcgtgacgca aaaaagaaga tgcgtaagtc cagcggttaa ccacatcttc ggcgaataga
     3181 cgcgagtgat caggatctgg cggtgggtga attccatcag gccggcaaag tcagtttcga
     3241 cgtaggaggc ggtcagacac tccgccagga agacaattgg cagactgcgc cgctgcaatg
     3301 cattggtcat cgaataatcg tcgctaacgg aatgttgcca ttcgtccagc acggcggact
     3361 gatcgaatac gcttcgtttg atcgccgttc cgccgcccca gcagaaattt ttagcggtat
     3421 tttcgccgag catggtgacg atgggagcat tccatgcagc cagtaggaga gaggcgaact
     3481 cattgttatt ggcgatgagc cagcgcatgg tggttgtggc gccgatccgg gaatcattca
     3541 gcggtccggt caacttacgc agccaggagc ggcccgggcg gccgtcggaa tcggcaaaga
     3601 ccaggacttc gaagtccgcg ggaagctgct cgatcgtggc gcggagattg ttgatctttt
     3661 cgccgcattc ccggggtgca cccgcaaaaa tgagatgcgc tttgccgcgt gagccagcta
     3721 ccacgcgctt cacgatcgcc gcggcggagt cggactccga ggccagcgca aaaaagactt
     3781 cgtagttttg atgatcgaac tcgcagagcg ctgtcagatt acgttccaaa cctggttcta
     3841 cgcctttgca gggacatagc acggcggttc ggggagagta gaaccccggg tcagtggcgg
     3901 cgcgtcggcg gacgtagccg agccagcgga tcgcctcgta caccaggtac agcccgacta
     3961 ggatctgcgc gagcgcaaga aaaataaaca agctattcat gcagacggag aataacaaac
     4021 gcgcacggct cgcgcagcac gcatattcgc aggtagaatc gcagaggtaa acggcggccc
     4081 agccaattct tcaggctgcc ctcgagagga gacatttatg ccggggcgcg tggattcatc
     4141 tatggtgacg acggcgctag agctgccggg ttatcgcatc gtgcgcaatt atggagtggt
     4201 gcggggcatc attgtccgct cgcgcagcgt aatcggaaac ctgggcgcgg cgctgcagac
     4261 catcgtcggc ggaaacatca ccatcctgac cgatctttgc gaaagaacgc gcatcgaggc
     4321 ctacgagctg ctgctgatgc acgcggcgga gcatggcgcg aacgcagtcg tcggttttcg
     4381 ttatgacgct acggaagtga tgcagggagt aaccgaagtg cttgcctatg gcaccgctgt
     4441 acaggtcgag ccacactcct gatttgctgc ttaggcgttt tctacggctt cggtcgccag
     4501 cttggccgcg atttcttcga cgctagccag cgcgcggctg gcagccaacc gcgccttttc
     4561 cagaaaatct gagtccagca tgaatgcgca acggtccaca aagggatggt tggccgcctc
     4621 gtaaggcttg cgcggacgac gcgacgacgc tgcgcgagcc aacaggtcct cgtacatttt
     4681 caagtctgat tgaaagtcca aggcgtcacc ggcgttccaa ttctgctgac gagccagtgc
     4741 ggaaagaaat tttcggcgga agctctccgc gcctttgcgc attactactt tctgtttccc
     4801 tcgtctgttc tcgatcgttt cgaacgtgga aatttccagt gcgcggacac aagcggccag
     4861 cagcgcttca cgctgcagga tggtgagcag gtcgcgataa aggggcgcat gaacctgcag
     4921 ggcgtgacgc ccattttcta tgacgtagga gtctacaaag cgcggaacaa cgcgctcgcg
     4981 cgccagcgtt tgcactagat tttgcgcgag caatgctggg gcctcgcctg c
//
LOCUS       DASTPM010000367         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_676178, whole genome shotgun sequence.
ACCESSION   DASTPM010000367 DASTPM010000000
VERSION     DASTPM010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676178"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2337
                     /locus_tag="VFR42_06420"
     CDS             <1..2337
                     /locus_tag="VFR42_06420"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEU0368823.1"
                     /translation="DVYEQVANAYIEGLTARAASGADVSRIASVASFFVSRIDSKLDA
                     QITNQLKTATDPIQRARLSGLLGKVAIANAKLAYHRFTELYSGEAWAALAKKGAQPQR
                     LLWASTSTKNPGYRDVIYVEELVGPSTVNTIPPATFNAFREHGRLRMSLTEDLENAAD
                     TMELVARAGISMKSVTDTLLDEGLRQFVDAFAQLLKATGSRKGQESTAKINSVSYTLP
                     AKLSAAVDSALTDWAKNDKVRRLWRADASLWSGSDENHWLGWLGITDDQVAHSAALTS
                     LAAEVKQAGFKHALLLGMGGSSLCPEVLRLTFGKIEGFPELHVLDSTDPAQIKAIEAR
                     IDLESSLFIVSSKSGGTLEPNIYKQYFFTRVKEVVGEKEAGNRFVAVTDPGSKMQHVA
                     ENDRFRHIFAGVPSIGGRYSALSNFGMVPAAVMGIDIVKLLNRTEEMVHACGATVPAT
                     ENPGVVLGAILGTAANSGHDKLTIIASPGISDLGAWLEQLLAESTGKSGKGIIPVDRE
                     SVARPDNYGTDRVFAYLRLESAPDQSQDAAVAALEKAGHPVVRIAVQDPYAIGEEFFR
                     WEIATAVAGSIIGINAFNQPDVEASKVATRKLTSEYEATGKLPAEEPFFEGDGVKLYA
                     DAKNADALKQGKSLAEILKTHFARAGQGDYFALLGYITMNPQNEAVLQAIRHSVRDKK
                     KVATVLGFGPRFLHSTGQAYKGGPNTGVFLQITCDNAVDLPVPDQKYTFGVVKAAQAR
                     GDFTVLAERGRRALRVHLGKDVKAGLATLQAAVGQALA"
     gene            2349..3407
                     /gene="gnd"
                     /locus_tag="VFR42_06425"
     CDS             2349..3407
                     /gene="gnd"
                     /locus_tag="VFR42_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU0368824.1"
                     /translation="MARKRRSGQEKSMQLGMIGLGRMGANMVRRLQRAGHQCVAFDRN
                     AESVRQVASDGAGAAASLDDLVKKLQRPRAVWLMVPAAVVDATLGELAGKLEAGDMVI
                     DGGNSYYIDDIRRAKELSTKGLHYVDVGTSGGVWGLERGYCQMIGGEKQIVGHLDPIF
                     KSLSPGRGDISRTPGREKVPGTAEEGYLHCGPSGAGHFVKMVHNGIEYALMAAYAEGL
                     NILKHANAGSGTREVDAETTPLRNPENYRYDFSLADVAEVWRRGSVVASWLLDLTAIS
                     LLEQPSLERFSGRVSDSGEGRWTILAAIESTAPAPVLTASLFQRFVSRGEDEFAAKLL
                     SAMRFQFGGHLEKAASGK"
     gene            3431..>3628
                     /locus_tag="VFR42_06430"
     CDS             3431..>3628
                     /locus_tag="VFR42_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368825.1"
                     /translation="MPDRTNMTESFNGPGHQASPCVMVIFGASGDLTKRKLIPALCNL
                     AKDKLLSPQFAVIGFSYNSMTT"
BASE COUNT          822 a         1043 c         1104 g          659 t
ORIGIN      
        1 gacgtttatg aacaggtcgc gaatgcttac atcgaaggtc tgaccgcgcg tgcggctagc
       61 ggcgccgacg tgagccgcat cgccagcgtc gccagtttct tcgtcagtcg cattgactcc
      121 aagctcgacg cccagatcac gaatcagctt aaaaccgcga ccgatccgat ccagcgggcc
      181 cgcctaagcg gcctgctcgg gaaagtcgca attgccaatg cgaagctcgc ctatcaccgc
      241 ttcaccgaat tgtattcggg agaggcctgg gcagccctgg ccaaaaaggg agcgcagccc
      301 cagcggctgc tgtgggccag caccagcacg aaaaatcccg gttaccggga tgtcatctac
      361 gtggaggagc tggtcggccc ttccacggtg aacacgattc ctcctgcgac tttcaacgca
      421 ttccgggaac acggcaggct tcgcatgagc ctgaccgaag acctggagaa cgccgcggac
      481 accatggaat tggtagcgcg cgccggcatc tcgatgaaat ccgtgacaga cacgcttttg
      541 gacgagggcc tgcgccagtt cgtggacgct ttcgcgcagc tcctgaaagc cacgggatcg
      601 cggaaaggcc aggaatccac agcgaagatc aattcggttt cctacacgct cccggcgaaa
      661 ctgtcggccg ccgtagacag cgcgctcacg gattgggcga agaacgacaa agtgcggcgt
      721 ttgtggcgag ccgacgcctc gctttggtcc ggatccgacg agaatcattg gctcggctgg
      781 cttggcatca ccgatgacca agtggcgcac agcgccgctc tgacttcgct tgccgccgaa
      841 gtaaagcaag ccggatttaa gcacgccctt ctgctcggca tgggaggatc gagcctttgc
      901 ccggaagtcc tgcggcttac ctttggaaag atcgaaggat ttcccgagct gcatgtcctc
      961 gactccaccg atcccgcgca gatcaaagcc atcgaagcca ggatcgatct agagagctcg
     1021 ctgttcatcg tctccagcaa atccggcggc acgctcgaac ccaacattta caaacaatat
     1081 ttcttcacac gggtcaagga agtcgtcggt gaaaaggaag cagggaatcg cttcgtcgcg
     1141 gttaccgatc cgggttcgaa gatgcagcat gtggccgaga acgataggtt ccggcacatc
     1201 tttgccggag tgcccagcat cggcgggcgt tattccgcac tatcgaactt cggtatggtc
     1261 ccggccgcgg tcatggggat cgacatcgtg aagctcctga accgcacgga ggaaatggtt
     1321 catgcctgcg gcgccaccgt accggcaacg gagaatcccg gcgtggtcct gggcgcgatc
     1381 ctcggaactg cagcgaacag cggacacgac aaactgacca tcattgcgtc tcccggtatt
     1441 tcggacctcg gagcctggct tgagcagctg cttgccgaat ccacggggaa aagcggcaaa
     1501 ggaatcattc cggtggaccg tgaaagcgtg gccaggcccg acaactacgg cacggatcgc
     1561 gtattcgcct atctccgcct cgaatcggca cccgaccaat cgcaagatgc cgccgtcgca
     1621 gccctcgaga aagcaggcca cccggtagtg cgcatcgccg tgcaagatcc ctatgctatc
     1681 ggcgaagaat ttttccgctg ggaaattgcc acagccgtag cgggttcgat catcgggata
     1741 aacgccttca accaacctga cgtggaagca agcaaggtgg caacccgaaa actcacgagc
     1801 gaatatgaag caaccggcaa actgcccgcc gaagagccgt ttttcgaagg cgacggtgtg
     1861 aagctctacg ctgacgcaaa aaatgcagac gccctcaagc agggaaaatc gctggccgag
     1921 atcctgaaaa ctcacttcgc gcgcgcgggc cagggagact atttcgctct gctcggctac
     1981 atcaccatga atccccagaa cgaggcggtt ctgcaggcca ttcgccacag cgtgcgggac
     2041 aagaagaagg tcgcgacggt gcttggattt ggtccgcgct ttctgcactc caccggccaa
     2101 gcctacaaag gcggacccaa taccggcgtc ttcctgcaga tcacctgcga caacgccgtc
     2161 gatcttcccg ttcccgatca aaagtacacc ttcggcgtgg tcaaagctgc acaggcccgc
     2221 ggcgatttca cagtacttgc tgagcgcgga agacgcgccc ttcgcgtgca cctcggcaaa
     2281 gacgtgaaag cgggattggc aacgttacaa gcggcggttg gccaggcact cgcataaagc
     2341 ggggagccat tgccagaaag cgcagaagcg gacaggagaa atcgatgcaa ctcggaatga
     2401 tcgggctggg aagaatggga gccaacatgg tgcgacggct gcaacgagcc ggacaccagt
     2461 gcgtggcctt tgaccgaaat gcggagtcgg tgagacaagt cgcgagcgac ggcgctggcg
     2521 cggccgcttc actggatgat ctggtgaaga agctgcaaag gccgcgagcc gtatggttga
     2581 tggttccggc ggccgtggtg gatgccacgc tgggtgagct ggcgggaaag ctagaagccg
     2641 gagacatggt gatagatggc ggaaactcgt actacatcga tgatattcgc cgtgcgaaag
     2701 aactctcgac caaaggcttg cattacgtgg acgtcggcac gagcggaggg gtatggggct
     2761 tggagcgcgg ctactgccag atgattggtg gcgaaaagca gattgtcggg caccttgatc
     2821 cgatcttcaa atcgctctcg ccggggcgag gcgacatttc gcggacgccg gggcgcgaga
     2881 aagttccggg aactgccgaa gaaggctatc tgcattgcgg cccgtcgggc gcggggcact
     2941 tcgtgaagat ggtgcacaac ggaatcgagt atgcattgat ggctgcctac gctgaagggc
     3001 tcaacatcct caagcacgcc aacgcgggca gcggaactcg cgaagtggat gccgagacca
     3061 ccccgctgcg caatccggaa aattaccgct acgacttctc tctagccgat gtggccgaag
     3121 tctggaggag aggtagcgtc gtggccagtt ggcttctcga cttgacagcg atttccttgc
     3181 tggagcagcc atcccttgag agattttccg gaagagtatc ggattccggt gagggacgct
     3241 ggacgatcct tgcggccatc gaatcgactg cgccagcacc ggttctcacg gcatcgctgt
     3301 ttcagcgttt tgtttcgcgc ggggaagacg aatttgcggc caagctcctt tccgccatgc
     3361 gatttcagtt tggcggccac ctggagaagg cggcgagcgg caagtaaatg gaccgggatt
     3421 ggaaaatcga atgccagaca ggacgaatat gactgaaagt ttcaacgggc ccggacacca
     3481 agcgagcccc tgcgtgatgg tgatttttgg agcaagcgga gatctgacca agcgcaagct
     3541 gattccggcg ctatgcaatt tggcgaaaga caagctgctc tcgccgcaat tcgcggtaat
     3601 cggcttctcc tacaattcaa tgacaacg
//
LOCUS       DASTPM010000368         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1252901, whole genome shotgun sequence.
ACCESSION   DASTPM010000368 DASTPM010000000
VERSION     DASTPM010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252901"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFR42_06435"
     CDS             complement(<1..798)
                     /locus_tag="VFR42_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU0368826.1"
                     /translation="MNPSSTRREEAIVGIFVIVAAALLIATLFSLTGFLNRGDVKYRA
                     FFKNAGGLRPGVGVSYAGGPPVGRVEEVRTDPHDSSRMEIIFRVHRDVPVRVDSLATI
                     TADSPLSDNHLGIVPKTGSTAALAPDGSVLESEEYIGFAQLEAEIGNLAPEAKKLLTN
                     LNERVTELQVTVARVNDLLNDKNRENLSVSMATVRGVLEENRKPLHATINNVNEASAK
                     IAPLLDDLKKTNAQLQKTLDTAEGTLDENRPDIRKSVEDLRRLLQTAN"
     gene            complement(889..1731)
                     /locus_tag="VFR42_06440"
     CDS             complement(889..1731)
                     /locus_tag="VFR42_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0368827.1"
                     /translation="MDELNTADAHGSAQAPALSIRELHVAYGEREILHGVSFDVLAGE
                     TVVILGGSGSGKSTLLRTLVGLEKPTSGEVILRGKNLCCVTDGEWRELRQHIGISFQS
                     GALFGSMTVGENVALPLLEHTQLDPATIEIMVRLKLNEVGLSGYEDYMPAQLSGGMKK
                     RAAVARAMAMDPEILFFDEPSAGLDPIIAAGIDQLLLKLKEAFGITIIVVTHELASAF
                     LIADRMVLLDKGHIVAVGTTAELQASTHPRVRQFLDRVAEPELSQEIDYLQMLTGEVP
                     RPRQ"
     gene            complement(1738..2532)
                     /locus_tag="VFR42_06445"
     CDS             complement(1738..2532)
                     /locus_tag="VFR42_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368828.1"
                     /translation="MPFTENIGSATLDGLAYVGGLAKLGASAARAVLRDLFERRPLAY
                     GRAVHQAMAVGIGSLPILSLITFFIGTILALQAAYELRKFGAMQFVADAVAISVSREL
                     GPLITAILVIGRSGSSFAAELGTMKVNEEIDALETMALDPVRFLVAPKLLSMLLMMPC
                     LTIWADFMGVVGGAVFGMAAAGFTFKTYIVATLNALALRDILTGLVKSLMFGLVITTV
                     GCQEGLRTGLGAEEVGRSTTSAVVKSIFLVIMVDLVFTAMFYIGIG"
     gene            complement(2572..2982)
                     /locus_tag="VFR42_06450"
     CDS             complement(2572..2982)
                     /locus_tag="VFR42_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0368829.1"
                     /translation="MPSGPPIKISTRQLGEVTILDLVGDITLFNSPQIRKVLLQLLRD
                     QQVAQLFVNMTDVKYVDSSGIASLVEGLKISRDLGSRFLLYGLSKPAKTVLELTHLLH
                     VFEVHANEQDALSALAPGPNNLARSAGDGSGESA"
     gene            complement(3102..>3635)
                     /locus_tag="VFR42_06455"
     CDS             complement(3102..>3635)
                     /locus_tag="VFR42_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368830.1"
                     /translation="ENRAQRAAFSSTLPHFSRRVSRPWSKAERAALFMQGSVLAIWIL
                     LPVSLASVILLWFFGRRLRVTAGVLHSRLCVFSDWCPVLLVAACAVLFLVYAPYDHAY
                     REILGSPLSPASYQDFGNAAYAPFALPPSVRAAMGSLCGPHGRFLVWSALTAVLVLLC
                     VILVMRQFKKPHFRRNG"
BASE COUNT          813 a         1158 c          960 g          704 t
ORIGIN      
        1 attggccgtc tgcagcagcc gtctcagatc ttccaccgac ttgcgtatgt ccggtcgatt
       61 ctcatccagc gtgccttccg ccgtatccag cgtcttttgc aattgcgcgt tcgttttctt
      121 cagatcgtcg agcagcggcg caatcttggc gctcgcttcg ttcacattat tgatcgtggc
      181 atgcagcggc ttgcgatttt cctccagcac gccgcgaacg gtcgccatgc tgacgctcag
      241 gttttcgcga ttcttatcgt tcagcaaatc atttacgcgc gcgactgtca cctgcaattc
      301 cgtcacccgc tcattcaaat tagtcagaag ctttttcgcc tccggtgcca ggttcccgat
      361 ttccgcttcc aactgcgcga agcctatgta ctcctccgac tccagtacac tgccgtcggg
      421 cgcgagcgcc gcagtgctgc ccgtctttgg cacaatgcct aaatggttgt cgctgagggg
      481 gctgtcagcc gtaatcgtcg cgagactatc cactcttacc ggcacatccc ggtggacgcg
      541 gaaaataatt tccattcgtg acgaatcgtg tggatcggtg cgcacttcct ccacccgacc
      601 gactggcggc cccccggcat agctgactcc aacgccaggc cgcaagcctc cggcattctt
      661 gaagaaagcg cgatacttca cgtcaccgcg gttcaagaat ccggtcagcg aaaacagcgt
      721 ggcgatcagc agcgctgccg caacaattac aaaaatgccg acaatggctt cttcgcggcg
      781 ggtggaggaa gggttcattt cagcggagac ctgtgaaaat ccgcaaaaag aaagcggagt
      841 atagcatgaa ggagaatgcg ccagtccgag cctgctgggc gctcatgttc attggcgcgg
      901 cctcggcact tctcccgtca gcatctgcaa gtaatcgatc tcctggctca gctccggttc
      961 ggccacccgg tcgagaaatt gcctcacgcg cggatgcgtg ctcgcctgca gttccgccgt
     1021 cgtccccacc gcaacgatgt gccctttgtc gagcagcacc atgcggtccg caatcagaaa
     1081 cgcgctggcc agctcatgcg tcactacgat gatggtaatt ccaaaagctt ccttcagttt
     1141 gagaagcagc tggtcgattc ccgcggcaat aatgggatcc agtcctgccg aaggctcatc
     1201 aaaaaaaaga atctccgggt ccatagccat cgcccgcgcg accgccgcgc gctttttcat
     1261 tccaccgctc agctgcgccg gcatgtaatc ttcatacccg ctcaagccta cttcgttgag
     1321 cttcagccgc accatgatct caatcgtcgc ggggtccagc tgggtgtgct ctagcagcgg
     1381 caacgccaca ttttctccaa ccgtcatcga cccgaacagc gcgccgcttt gaaaggatat
     1441 tccgatgtgt tgccgcagct cccgccattc tccatcggta acgcagcaaa gatttttgcc
     1501 gcgcaaaatc acttctccgc tggtcggctt ttccagcccg accagcgttc tcagcaacgt
     1561 gctcttgccc gaacccgagc cgcccagaat cactaccgtc tctccggcca gcacatcaaa
     1621 cgaaacccca tgcagaattt cacgctctcc atacgccacg tgcagttcgc gaatcgacag
     1681 cgccggcgct tgcgccgagc cgtgcgcgtc tgcggtgttg agttcgtcca tcactcgtca
     1741 gccgatcccg atgtaaaaca tcgccgtaaa caccaggtcc accatgatca ccagaaaaat
     1801 tgatttcaca acagcagacg tcgtcgaacg gcccacctct tccgcgccta ggcccgtgcg
     1861 caatccctct tggcaaccaa ccgtcgtgat caccagcccg aacatcagac tcttgaccag
     1921 accggtgaga atatcgcgca gcgccagcgc gttcagcgtc gccactatgt acgtcttgaa
     1981 cgtaaagccc gccgctgcca tgccgaagac cgcgccccca accacgccca tgaaatccgc
     2041 ccaaatcgtc aggcacggca tcatcagcag catggaaagc agtttcggcg ccaccagaaa
     2101 ccgcaccgga tcgagcgcca tcgtttccag tgcatcaatt tcttcgttca ccttcatggt
     2161 gccgagttcc gccgcgaagg aagagccgct ccgaccgatg acgagaatcg ccgtgatcag
     2221 cggaccgagc tcgcgcgaaa ccgaaatcgc caccgcatcc gcaacaaact gcattgcgcc
     2281 gaattttctc agttcatacg ccgcttgcag agccaggatc gtcccgataa aaaaagtgat
     2341 cagcgaaaga atcggcaaag agccgattcc caccgccatg gcctggtgca ctgcgcgccc
     2401 gtaggccagc ggccgccgct cgaataggtc cctgagcacg gcgcgggccg cggatgcgcc
     2461 aagcttcgcc agcccgccga cgtaagccaa tccgtcaagc gttgcgctgc cgatattttc
     2521 cgtaaaaggc attttcagtt gtgaaagttt ccggcgattg tccccgcagg attatgcact
     2581 ttcaccggag ccgtcgccag ccgagcgcgc gaggttgttg ggccctgggg ccaaggcgct
     2641 caaagcgtcc tgctcgttgg cgtgaacttc aaaaacgtgc agcaggtggg tcagttcaag
     2701 caccgtcttc gccggcttcg agaggccgta aagcagaaaa cgcgagccaa ggtcgcgcga
     2761 gattttgaga ccctccacca gcgacgcaat tccggaactg tccacgtact tcacgtcggt
     2821 catgttgacg aagagctgcg cgacttgctg gtcccgcaag agctgcagca gaactttgcg
     2881 aatctgcggc gaattaaaca aagtgatatc cccgacgagg tcgaggatgg tgacttcgcc
     2941 cagctggcga gtggagatct tgattggagg acctgagggc accgtgcctt gttttgccag
     3001 agaatggcgt cagatgcaag cccccatcga atattcgcaa caaacgccgg aaattactgc
     3061 caacaggaag cgttcgacat tgccaacgga cctaaaagtg cttagccgtt tcgacggaag
     3121 tgcggcttct tgaactgccg catgaccaga atgacgcaca gaaggaccaa caccgcagtc
     3181 agcgcggacc acaccagaaa ccgcccgtgt ggtccgcaaa gtgaacccat tgctgcgcgg
     3241 acactcgggg gcaaagcaaa cggagcgtat gcggcatttc cgaagtcctg atagctggcg
     3301 ggagatagcg ggctgcccaa aatctcgcga tacgcatggt cgtagggcgc gtacacaagg
     3361 aaaagaaccg cgcaggctgc cacgagcagc acagggcacc aatcggaaaa aacgcacaaa
     3421 cgagagtgga ggactcccgc agtaacgcga agcctgcgcc cgaaaaacca cagcaggatg
     3481 acgcttgcca acgaaaccgg aagcagaatc caaatcgcga gcacgcttcc ctgcatgaac
     3541 agtgcggccc gttccgcttt cgaccagggg cgcgacacgc gcctcgaaaa gtgcggcagg
     3601 gtgctcgaaa atgccgcgcg ctgtgcacga ttttc
//
LOCUS       DASTPM010000369         5001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_215141, whole genome shotgun sequence.
ACCESSION   DASTPM010000369 DASTPM010000000
VERSION     DASTPM010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215141"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(481..1350)
                     /locus_tag="VFR42_06460"
     CDS             complement(481..1350)
                     /locus_tag="VFR42_06460"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0368831.1"
                     /translation="MDVKTIGVIGAGTMGRGIAYAAALGGYRTILEDVMPENLVNGTT
                     WIKKALEEGVTRGKVTAEDSTAALRRIEAVSTVEAACRESDFLIEAVPEEMELKLELY
                     TLFDKFARPNAVMASNTSSLSIAEMAEMTYRPELCVGMHFFNPVPKMRLIEIVRAPKT
                     SDETIAACREVGARMGKEVVVVKESPGFITSRVNALIGNEAFAMLEAGVASPEDIDKA
                     VKLGLNHPMGPFEMVDLVGLDVRLRILEYLHSTLGEKYRPNELLKKLVADGRLGHKSG
                     RGVYDYTQLKPTK"
     gene            complement(1393..1692)
                     /locus_tag="VFR42_06465"
                     /pseudo
     CDS             complement(1393..1692)
                     /locus_tag="VFR42_06465"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
     gene            complement(1791..2114)
                     /locus_tag="VFR42_06470"
     CDS             complement(1791..2114)
                     /locus_tag="VFR42_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0368832.1"
                     /translation="MSELEISVAELQKRKKAGQPFLLIDVREPWEYETCRIEGAQLIP
                     MRTIPANLQRLDVDQDVICYCHHGQRSLDVAAWLRGQGIESAQSLAGGIDSWSMEIDP
                     TVPRY"
     gene            complement(2133..2948)
                     /locus_tag="VFR42_06475"
     CDS             complement(2133..2948)
                     /locus_tag="VFR42_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0368833.1"
                     /translation="MNPVQTAPAAGLVLLETVQSGIALLVMNRPDKLNALNNELATAL
                     NQAFERIGKDDSVRVVVLTGAGRAFCAGGDLGMIGKGREARDPKQLEPILRAGMGAVL
                     RMRTIQQPVIAAVNGAAAGAGMNIALAADIRIAAEDAAFGQNFAKVGLFPDYGGTYFL
                     PQLVGPSKAAELFYTGDMIDAQTALRLGLVNRVVPAAQLEAEVKAYAARLAAGPPVAM
                     RAVKQTLFGADKEALKHALEGEVVQQMRCFASEDSLEGIHAFFEKRAANFKGR"
     gene            complement(2967..3497)
                     /locus_tag="VFR42_06480"
     CDS             complement(2967..3497)
                     /locus_tag="VFR42_06480"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEU0368834.1"
                     /translation="MKTLPDHLRKGMKLVIVGCNPTESSVRTGHYYSGRGNPFWPVLF
                     ETGVIPEPLEYRDDKRIIEFAIGLTDLVKRPTRTIEELTREDYAEGRIVLSQKLGEYV
                     PHVIAFNGKSVYEQFAQRRVKLGLQKETLYGASIYVLPATDAQHTQGSKERTVHFRKL
                     AALVRKVEKARDARPA"
     gene            complement(3565..4533)
                     /locus_tag="VFR42_06485"
     CDS             complement(3565..4533)
                     /locus_tag="VFR42_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEU0368835.1"
                     /translation="MTLHINEAEVRSVLTMPLAMEAVETVSKRQAAGEVLVHPRRRFE
                     LPDRAFFNYMAAADVADNFVATKQYTYVGGKLKFVVSLFSTESGDLLALIEADVMGQM
                     RTGAASGVATKYLARTDGKTAAIIGTGWQARTQLEAIAAARKLERVKVYGREAERREK
                     FAREMSASLGITVEPAASSAEATAEAAIVCTATTASQAVVNAGSIAPGTHINAIGANH
                     VKKRELDAETVVKCDRIFVDSIEQARQEAGDLVLGFAGHEKRWDTVRELSSLVAGICQ
                     GRTSPGQITLFKSNGIAAWDLAAAISVYRLAKDRGLGRTLPFWQTS"
     gene            complement(4560..>5001)
                     /locus_tag="VFR42_06490"
     CDS             complement(4560..>5001)
                     /locus_tag="VFR42_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368836.1"
                     /translation="WVIARFLELDVPDPVREGANAANLRPIAWFELNRVEDPSGIKQQ
                     YLLAAARGAEGQACDFSMLRVYTWNRKKSRYETAFIENNLCGALPIRIGKSTAGQPEF
                     RFMNADDRKNARVYRLMQTVVRRVREGDDTGKAQATGKSRTKGH"
BASE COUNT          959 a         1643 c         1266 g         1133 t
ORIGIN      
        1 tgcgcctgat gctgggccag gtggcggaag ccagccagga cgcacaaacc gctttacgcc
       61 tcgatcccag caatgcccag gcacgcagcg tgctcgaacg tccgcaggcc ggtagcctgc
      121 actgagccgt ctgtacgctg cgctactacg cgggacgata cgccttgcag cggcaacccg
      181 gaaagttgca ctgcgtccct agcttctggt tggcgtacat tcgagcatga atgctcttgg
      241 gatgatgaca ctcgcaacgc aggagagact tgcggcctct cgttgcgccc ttgctgaccg
      301 aagatctgcc gctgctacca cttccctgcc gacccggtgt agacacttgt ggacctccga
      361 taccgaaggg gcccggagtc catccgccac ggccaaagca ggccgcagtg gataactgcc
      421 tcgttatatc acagtttttt atttttcaga gggctgagtc gcagacgccg tctacggcgg
      481 ctattttgtt ggcttcagct gtgtgtagtc atacacgccg cgaccgctct tgtggcccaa
      541 acgcccgtcc gccaccagct ttttgaggag ttcattggga cgatactttt cgcccagcgt
      601 ggaatgcagg tattcgagga tgcgcagccg cacatccagt cccaccagat ccaccatttc
      661 aaacggaccc atgggatggt tcagccccag tttcaccgcc ttgtcgatgt cttctggcga
      721 ggccaccccc gcttccagca tggcgaacgc ttcgttgccg atcagcgcgt tgacgcgact
      781 ggtgataaat ccaggcgatt ctttgaccac cacaacttct ttgcccatgc gggcgccgac
      841 ttcgcggcaa gcggcgatcg tttcatcgga agtcttcggc gctcgcacaa tttcaatcaa
      901 ccgcatcttc ggcaccggat tgaagaagtg catgcccacg caaagttctg ggcgataggt
      961 catctccgcc atttccgcga tcgacagtga agacgtattg ctggccatca ctgcgttggg
     1021 acgcgcgaac ttgtcgaata gcgtatacag ttccagcttc aactccatct cttccggaac
     1081 cgcttcgata aggaagtcgg attcgcggca cgcggcttct accgtagaaa ccgcctcgat
     1141 tcgccgtagc gctgcggtac tgtcttccgc agtcaccttg ccgcgtgtta cgccttcttc
     1201 cagcgccttc ttgatccaag tggttccgtt gacaagattt tccggcatca cgtcttccag
     1261 aattgtgcgg tatccaccaa gcgcggcggc gtaggcgatt ccccgcccca tggttccagc
     1321 gccaatcacg ccgattgttt tcacgtccat ctgtcatcac tctccggtga aaagagtagc
     1381 tacgggcgat gcctacttca aaagtttgcg ataggcggct tcatcgaggc ccagaacgat
     1441 ctggtcgcct cgcaccacta ccggccgctt gatcaggttc ggattgcgcg acatcaggcg
     1501 aatcgcttcc gccctcgatg gcgtcttctt tgccatagtc ctctcgcgat acagttcatt
     1561 tcgcggattc agaaacggtt cgtaatcatg ggtgccaatc aactcgtcca gctctttatc
     1621 cgacattggc tgcttatcca gatcgcgcga ctccagttcc gctcccagct ccagcaagag
     1681 acctcttgct ttgcggcaag tcgttcaagt gggcttctga aagaacttca ctttgatttt
     1741 tggcatgggt tctccctaag gtttgtccac gtaagaattg ttttcaacgg ctagtagcga
     1801 ggaacagtag gatcgatttc catcgaccaa gaatcgattc cgcccgccaa cgattgcgcg
     1861 gactcgatgc cttgcccgcg cagccaggct gctacatcca ggctgcgttg cccgtggtgg
     1921 caatagcaga tcacatcttg atccacatca agtcgctgca aattggcggg aatggttcgc
     1981 atgggaatca gctgcgcgcc ttcaatgcgg caggtttcgt attcccatgg ttcgcgcaca
     2041 tcaatcagta aaaatggctg gccggctttt ttccttttct gcaactccgc aacgctgatc
     2101 tccaattcgc tcattcttca attgtagcgc gatcagcgtc ctttgaaatt tgccgcccgc
     2161 ttctcaaaaa atgcatggat gccttcgagc gagtcttccg aagcgaagca gcgcatctgc
     2221 tgcaccactt ccccttccag cgcgtgcttc aaggcttctt tatctgcgcc gaagagcgtc
     2281 tgtttcactg cgcgcatggc cacgggcggc ccggccgcca gcctcgccgc atacgccttc
     2341 acttccgctt ccagctgtgc agcgggcacc acccggttca ccaaaccgag tcgcaacgca
     2401 gtctgcgcat cgatcatgtc acctgtataa aaaagttccg ccgccttaga cggcccaacc
     2461 agctgcggca aaaaatacgt tcctccataa tccggaaaca atcccacctt ggcaaaattc
     2521 tgaccgaacg ccgcatcctc cgcggcaatc cggatgtccg cagccagcgc gatgttcatt
     2581 cccgctcccg cggcagctcc gttcaccgcg gcgatcaccg gctgttggat agttcgcatg
     2641 cgcaacaccg cgcccatgcc tgcgcgcaga atcggctcga gttgtttcgg atcgcgcgct
     2701 tcgcggccct tgccgatcat gcccagatca ccgcctgcac aaaacgctcg gcccgctccg
     2761 gtcagtacga ccacacgcac cgaatcgtcc ttgccaattc gctcaaacgc ctgattcagc
     2821 gccgtcgcca gctcattgtt cagcgcgttc agcttgtccg gccggttcat caccagcaaa
     2881 gcgatgccgc tttgtacggt ttccagcaac acgagtcccg ctgcgggcgc agtttgcaca
     2941 gggttcatac tttcctccag gagatttcag gcgggtcggg cgtcgcgcgc tttttccact
     3001 ttgcgcacca gggcagccag cttgcggaaa tgcacagtcc gctctttcga cccttgcgta
     3061 tgctgcgcat cggtagccgg caaaacatag atactcgctc cgtacagagt ttctttttgc
     3121 aggcccagct tcacgcggcg ctgcgcaaac tgctcgtata cgctcttacc gttgaacgca
     3181 atcacgtgcg gaacatattc gcccagcttc tgcgaaagga cgattcgtcc ctccgcatag
     3241 tcctcgcgcg tcagttcctc gatcgttcgc gtgggccgct tcaccaggtc cgtcagacca
     3301 atcgcaaact cgatgatgcg cttgtcgtcg cgatattcca atggctcggg gatcactccc
     3361 gtctcaaaca gaacaggcca aaaaggattg ccccgtccgg agtaatagtg ccctgttcgc
     3421 accgacgact cagttggatt gcatcctacg ataacgagtt tcattccttt gcggaggtgg
     3481 tcgggcagag tcttcatggc tcaagcttct ttcgtgtgcg caactagaat agcccgaccc
     3541 ttacccctcg accgaaggct tgttctagct ggtctgccaa aaaggcaacg tccggcccag
     3601 ccctcggtcc ttcgccaggc gatacacgct aatcgcggcg gccaggtccc aggcagcgat
     3661 tccgttggat ttgaaaagtg taatctggcc gggactggtt cgcccctggc agatgcccgc
     3721 caccagtgac gacagctcgc gcacggtgtc ccagcgtttt tcgtgaccgg cgaagcccag
     3781 caccagatcg ccggcctcct ggcgcgcctg ctcgatggaa tccacaaaaa tgcggtcgca
     3841 cttgacgacg gtttcggcgt ccaactcgcg cttcttcacg tggttagcgc cgatggcatt
     3901 gatatgcgtc ccgggagcaa tgctccccgc gttgactacc gcctggctag ctgtcgtcgc
     3961 cgtgcagaca atcgcggctt ccgccgtcgc ttcggcggaa ctcgcggcag gttcgaccgt
     4021 gattcccagg ctcgccgaca tttctctggc gaacttttcc cgccgctccg cctcgcgccc
     4081 gtacactttt acgcgttcca acttgcgcgc cgcggcaatc gcttccagtt gcgtccgcgc
     4141 ttgccagccg gtgccaatga tcgctgctgt tttcccatcc gtccgcgcca gatattttgt
     4201 ggccacgccc gaagccgctc ccgtccgcat ctgtcccatc acgtcggcct cgatcaacgc
     4261 cagcaagtct ccgctttccg tcgagaacag cgacaccaca aacttcagtt tcccgcccac
     4321 gtaggtgtac tgcttcgtcg cgacaaaatt atctgccacg tccgccgccg ccatgtaatt
     4381 aaaaaacgca cggtccggca attcaaagcg gcgccgcgga tgtaccagca cttcgccagc
     4441 cgcctgccgc ttcgaaaccg tctccaccgc ttccatcgcc agcggcatgg tcagcacgct
     4501 tctcacttcg gcttcattaa tgtgcagtgt catggctctg aactctcccg ccgcaaaaat
     4561 cagtgacctt tcgtccgcga ttttccagtg gcttgtgctt tgccggtgtc gtcaccctcg
     4621 cgcacccgcc gtaccaccgt ctgcatcagc cgatacaccc gcgcattctt acggtcatcc
     4681 gcattcataa agcgaaactc cggctgcccc gccgtcgact tgccaattcg gatcggcaag
     4741 gcgccacaca aattgttttc aatgaatgcc gtctcgtagc gcgatttctt gcgattccag
     4801 gtatagacgc gcagcatcga aaaatcgcat gcttggccct ccgccccgcg tgccgccgcc
     4861 aataaatatt gctgttttat gccagaagga tcttccacgc gattcagctc gaaccatgcg
     4921 atcggccgca aattcgccgc gttggcccct tcgcgcaccg ggtccggcac atccagttcc
     4981 aaaaatcgcg cgatcaccca g
//
LOCUS       DASTPM010000370         2950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1223934, whole genome shotgun sequence.
ACCESSION   DASTPM010000370 DASTPM010000000
VERSION     DASTPM010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223934"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="VFR42_06495"
     CDS             complement(<1..1378)
                     /locus_tag="VFR42_06495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU0368837.1"
                     /translation="MLKPFFRRWAHGTVALAFCVSSFPAAGEAAATKLKLRGYLTARA
                     DANAMLILDDRIELTSSSRVLSKDSSGEHAFSPQELAPGMLVEAEGQWLDKHKFFAEK
                     LTVDLGEDDKKIHGSAYLQEEPADATRIAAGGASELKLDGYWLRLDAGSKRSWNAARA
                     AQGLPAKTPGESTALAGYRVKYAGTRGKDGKIAAEEVELGPPAPADDYKMPHNLEIVR
                     AKDPLTGIDVVEFRQGNKVQGRMKLLAERTVQEYVSQLGDSLIPEGAKGARRPIEFRF
                     FVVEDPEINAASLPDGTLLINTGLLGALQNEAQLAFVLSHEMAHVLQVHYRREIEETR
                     GTRIGLTIAGLAAGAFIGNAGVFMAQIGIASVVNGHQRELENQADRLGLQNVIEHGYD
                     PREASNFSRIMISRYGDRTASKLWSNHDSSVIRGSFLTVQLMREYPEGHWDTARKNTP
                     AFLTMKEDL"
     gene            complement(1397..1933)
                     /locus_tag="VFR42_06500"
     CDS             complement(1397..1933)
                     /locus_tag="VFR42_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HEU0368838.1"
                     /translation="MRIFTVAVVGLMFAIGAAARPKDEAPQYKTAEAKHFTTAEGVEL
                     SPAFVDYFYAELRNELTKGKLVKEVIGEGEAVEDADAPKSLVISGTVTEYKKGSVVKS
                     ALIGFGAGFRSLKMDADVVRRSDKQNLCVIHVHVKIDPRWNEKVMAKGAAHEMVKEMK
                     KALAEGAKAATAEAAKPS"
     gene            complement(2016..2702)
                     /locus_tag="VFR42_06505"
     CDS             complement(2016..2702)
                     /locus_tag="VFR42_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368839.1"
                     /translation="MPLRGTLRLSIVLLSLVAFSALTMQLAQADEPKPKPENTISIQP
                     GDPVPPQKFERPPKFWIADITDRSGNPQPLLVMKERGGVFLDKQPTAIVKDALEQSLK
                     AANLLASDATSADLVLRVYLFHFGLASGSALDFFGKVEFSTMVKNPKTGESLEVKAVG
                     TSIANGAMRKKNMQKNVEENIEAALRDATRNFLRGTQLKEAVATLAKGAEVVPVAAPA
                     PVEPPKPQPR"
BASE COUNT          544 a          842 c          872 g          692 t
ORIGIN      
        1 cgaggtcttc tttcatggtc aggaacgcgg gagtgttttt cctggcggtg tcccagtggc
       61 cttcggggta ttcgcgcatc aactgaacgg ttaaaaagct tccacgaata acgctggaat
      121 cgtgatttga ccagagctta ctggcggtgc gatcgccgta tcggctaatc atgatgcggg
      181 aaaagtttga cgcttcacgc ggatcgtagc catgctcaat cacgttctga aggcccaggc
      241 gatctgcctg gttttcgagt tcacgctggt ggccgtttac cacggacgca atcccgatct
      301 gtgccatgaa gactcccgcg ttgccgatga aggcgcccgc ggcaaggccc gcaatggtca
      361 gaccgattcg tgtgccgcgg gtttcttcga tctcgcgacg atagtgcacc tgaagaacgt
      421 gggccatctc atggctcaac acgaatgcga gctgcgcttc attttgcagc gcgccaagca
      481 aaccggtatt gatcaggagt gttccgtcgg gcagcgaggc ggcgttgatt tccggatctt
      541 ccacgacgaa gaagcggaac tcgatagggc ggcgagcacc tttcgcgccc tcgggaatca
      601 gggaatcgcc caattgcgag acgtattcct ggacagtccg ctccgcgagc aacttcatgc
      661 gcccttgcac tttgtttccc tggcgaaatt cgacaacgtc aattcccgtt agtggatctt
      721 ttgcgcgaac gatctcaagg ttatgcggca tcttgtagtc gtcagcgggc gcgggaggcc
      781 cgagttcgac ttcctccgct gcgatcttgc cgtccttgcc gcgcgtgccg gcatacttca
      841 cgcgatagcc cgcaagcgcc gtgctttcgc ctggcgtctt ggccgggaga ccttgcgcgg
      901 ctctagctgc gttccatgag cgcttggaac ctgcatccag acgcaaccag tagccatcga
      961 gcttgagttc gctggccccg ccagcggcaa tcctggtggc gtcggcaggt tcttcctgca
     1021 aataggccga gccgtgaatc ttcttgtcgt cctctccgag gtcaaccgtg agcttttcgg
     1081 cgaagaactt gtgtttgtcg agccactgcc cttccgcttc caccaacatt ccgggagcaa
     1141 gctcctgcgg actgaaggcg tgttcaccgg aactgtcttt gctcaaaacg cgactggagg
     1201 aggtcaactc gattcggtcg tcgaggatca gcatggcgtt ggcatcggcg cgagcggtga
     1261 ggtatccgcg caacttcaat tttgtggccg cggcctcacc ggcggcagga aaagaagaga
     1321 cacaaaatgc gagcgcgact gtgccgtgcg cccaacggcg aaagaatggt ttcagcatgt
     1381 acaagctcgc tggaccctat gagggtttgg ctgcctcggc ggttgctgct ttcgctcctt
     1441 cggccagggc ttttttcatt tccttgacca tttcgtgtgc ggcgcccttg gccatgacct
     1501 tttcattcca gcgcgggtca attttcacgt ggacgtgaat gacgcagaga ttctgcttgt
     1561 cgctgcggcg aaccacgtcg gcgtccatct tgaggctgcg aaaaccggcg ccaaatccga
     1621 tgagcgcgct cttcaccacg cttccttttt tgtattcggt gacggtgccg ctgatcacca
     1681 aagatttcgg cgcgtcagcg tcttccacgg cttccccttc accgataact tctttcacca
     1741 gcttgccttt ggtcagttcg ttgcggagtt cggcataaaa gtagtccaca aacgccggac
     1801 tcagttcgac gccttcggca gtggtgaaat gtttggcctc ggcagttttg tattgcggag
     1861 cttcgtcctt tggtcttgct gccgcaccga ttgcgaacat gagtccgacc actgctaccg
     1921 tgaagatgcg catttgcttc tttctcctct cctggtccct tgcgaattca gtgccttcct
     1981 tacgatttat cagccgagtt cagatgctga aacccttatc gcggctgcgg tttcggcggt
     2041 tccaccggag caggtgcggc caccggaacg acctccgctc ctttcgccaa agtggcgacc
     2101 gcttctttca gctgcgtacc tcgcaggaaa ttccgggtgg cgtcgcgtaa ggcggcttcg
     2161 atgttttctt ccacgttttt ttgcatgttt ttcttgcgca tcgcgccatt ggcaatggaa
     2221 gtgccgaccg cttttacttc gagggactct cccgttttcg gatttttcac catggtggag
     2281 aattcgacct tgccgaagaa atcaagcgcc gaaccggaag ccaggccgaa gtgaaagagg
     2341 tagacgcgca gaacgaggtc ggcggaagtg gcatcgctgg ccagcaggtt cgcagctttg
     2401 agcgattgct cgagcgcgtc tttcacaata gcggtaggct gcttgtcaag gaatacgccg
     2461 ccgcgctctt tcataaccag cagcggctgg ggatttccgg aacgatcggt gatgtcggca
     2521 atccagaatt tgggcgggcg ctcgaatttt tgcgggggaa cgggatcgcc cggctgaatc
     2581 gagatggtgt tctcgggctt gggctttggc tcgtcggctt gcgccagttg catcgtcagg
     2641 gcggaaaacg cgacgagcga cagcagtacg atcgacaaac gcaaggtccc gcgaaggggc
     2701 atgggtgttc tccgcctggc agtggtggtc gtcaatccgc tgccgtgttt tgatggccgt
     2761 tggcctagtc gcgagccgcg tggctcgcct gctcgaacga tggagacgag gctatacgcc
     2821 tgcgtgagtc accgcaagga aggagaagta cagttgtgcc gaaacagaga tggggcaatc
     2881 cgccaacagg aaacgccttg ccatcccgaa ctggcccacc gtagcgggtc gagtgcgcca
     2941 cccgtgcggc
//
LOCUS       DASTPM010000371         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_272675, whole genome shotgun sequence.
ACCESSION   DASTPM010000371 DASTPM010000000
VERSION     DASTPM010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272675"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..1711)
                     /gene="glmM"
                     /locus_tag="VFR42_06510"
     CDS             complement(371..1711)
                     /gene="glmM"
                     /locus_tag="VFR42_06510"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU0368840.1"
                     /translation="MPKQYFGTDGIRGVPGTPPLDDATLHAVGQAVGAYLHKKHSEPH
                     ALIGMDTRESGPHMAGLLTAGLEEAGVDVTLAGVLTTPGVACLVRMNDFHAGVVISAS
                     HNPFHDNGVKLFSHAGMKFPDRAEEEIEAEISRFLKEAAVSSHALTADESLHQEYLEF
                     LRSRVLEGAKLQGMRIVLDCANGAAYKLGPELFRSLGCDVVTVGTEPDGRNINKGCGS
                     LHLEDLQRRVPAEKAALGVAFDGDADRALFVSSAGKIVNGDGVLLAAARFLKKAGKLT
                     GNRVVATSMSNLGLERVLSHEGIGLARTNVGDRYVLEEMLKSGNALGGEQSGHIIFLE
                     DSPAGDGLLTAVKVTSLVALHGSLEALVEGLKDYPQTIVNVKVKTKPALESVPEVAQA
                     LREAETTLGDNGRIVLRYSGTEPLARVMVEAEHSGDVERFSQSIATAIRGTIGI"
     gene            1900..>2362
                     /locus_tag="VFR42_06515"
     CDS             1900..>2362
                     /locus_tag="VFR42_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0368841.1"
                     /translation="MLRKTELRLPATEAPGETTAAKEYIRVAGSPFSLRVIAAAIILL
                     FAYYAQGVVITLVLSILLAYCLDPLVEFLERWRIPRTVGAMIIVLLLCVVLGAVTYSL
                     WTRTSDFAENWPKYSGVWRQTAASIESKIFPGTPVVTGTQAPATALSPRDSE"
BASE COUNT          460 a          756 c          643 g          503 t
ORIGIN      
        1 cccggctctc tggaacgcta tggcaagaag gtgggtccga aattgagcga cactgttcct
       61 accggctcgc agcatgcagc accggcaaaa aagcttcctg catcaattct gcagcttgag
      121 cccgatctgt caggcggcga ccataaaatg cgtagatcgg ttggcgatag cgattaaaca
      181 gttcgtcaaa acaggccttg tcctggtgca cgaatgcgag catcagctgc tcgtcggagg
      241 tttggccgtt gtggtgggca gggcaaagat tgacaaagct gtctccagat tgagaaaccg
      301 tggctcgcga cggcgcagcc gaggcccccc ctggtaatgt cgctaggctt ccaatagttc
      361 ctagaatgga ttagatgccg attgttccgc gtattgccgt ggcaatggac tggctgaatc
      421 gctctacgtc gccagagtgt tctgcctcga ccatgacgcg ggccagaggt tccgtgccgg
      481 agtaacgcag cacaatgcgg ccgttatcgc ccagcgttgt ctccgcttca cgcaacgcct
      541 gcgccacttc cggcaccgat tcgagagccg gcttggtttt cactttcacg tttacaatcg
      601 tctgcgggta gtccttcagg ccttcgacca gcgcctccag gctgccatgc agcgccacca
      661 gcgacgtcac tttcaccgcc gtaagcagcc catctcctgc cggcgaatcc tccagaaaaa
      721 taatgtggcc agactgctcg ccgcctagcg cattgccgct cttcaacatc tcttccagca
      781 cgtagcggtc acccacattg gtgcgtgcca gcccaatccc ttcgtgcgaa agcacccgct
      841 ccagtcccag gttggacatc gacgtcgcca ccacgcggtt ccccgttaac ttccccgctt
      901 ttttcagaaa gcgcgccgca gccagaagaa ctccgtcgcc gttcacgatc tttcccgcgg
      961 agctcacaaa gagcgcgcgg tcagcgtcgc cgtcaaacgc cacgcccagc gcggcttttt
     1021 cggcaggcac gcggcgctgg agatcttcca aatgcagtga tccgcagcct ttgttgatgt
     1081 tgcgcccgtc cggctcggtt ccgaccgtaa ccacatcgca gccgagcgag cgaaatagtt
     1141 cgggccccag cttgtacgct gcaccgttcg cacagtccag cacgatccgc atgccctgca
     1201 atttcgcgcc ctcgagaacg cggctacgca agaattccag atattcctgg tgcaacgatt
     1261 catccgccgt cagcgcatga gacgaaaccg ccgcttcctt tagaaatcgc gaaatctccg
     1321 cttcaatctc ctcctccgct cggtcgggaa acttcatccc cgcatgggag aacagcttga
     1381 cgccgttgtc atgaaacgga ttgtgcgaag cggaaatcac cacgcccgcg tgaaaatcgt
     1441 tcatccgtac caggcaggca acgcccggcg tcgtcagcac acccgccagt gttacgtcca
     1501 ctccagcctc ttctagcccc gccgtcagaa gtcccgccat atgcggaccc gactcgcgcg
     1561 tgtccatgcc gatcagcgcg tgcggctcgc tatgtttttt gtgcaggtat gcgccgaccg
     1621 cttggcccac ggcatgcagc gttgcatcgt ccagtggagg agttccgggg acaccgcgaa
     1681 tgccgtcggt tccaaaatat tgtttgggca tagagaaatc gggaagtgca gccaggcgcg
     1741 cactctccaa aaaagtttag cacagcgacc gccttcccct gccggcgaga gccgccattt
     1801 cgctgcgcga aacgtcgcgc gcctccggca acttcaggct tgcatcgtgt gttaccttgt
     1861 ttgtctaagg aaggtctcag agctttcttc gagcactgag tgttgagaaa gacggagttg
     1921 cgcttgcccg ctacggaagc tcccggggaa accacagccg ctaaggaata tatccgcgtg
     1981 gctggttcgc ctttcagcct gcgagtgatc gctgcggcca tcattctgct cttcgcgtat
     2041 tacgcgcagg gtgtcgtaat cacgctcgtc ctgtccatcc tgctggctta ctgcctcgat
     2101 ccgctggtgg aattcctgga gcgctggcgc atccccagga ctgtcggcgc catgatcatt
     2161 gttctgctct tatgcgttgt tctcggcgcg gtcacctaca gcctgtggac ccgcacttca
     2221 gattttgccg agaattggcc caaatacagc ggggtatggc ggcaaactgc agcttccatt
     2281 gaatccaaga ttttcccggg cacgccggtt gtaacgggga cacaggctcc ggcgacggcg
     2341 ctttcgccgc gcgactccga ga
//
LOCUS       DASTPM010000372         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_128695, whole genome shotgun sequence.
ACCESSION   DASTPM010000372 DASTPM010000000
VERSION     DASTPM010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128695"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFR42_06520"
     CDS             <1..204
                     /locus_tag="VFR42_06520"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU0368842.1"
                     /translation="VGGKPKQNRWTARTGVPAKTPESDAMSKDLKKRGFRFIGSTICY
                     AFMQAVGMVNDHAITCFRYNSLK"
     gene            complement(211..1179)
                     /locus_tag="VFR42_06525"
     CDS             complement(211..1179)
                     /locus_tag="VFR42_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HEU0368843.1"
                     /translation="MRKHFSSTLVVAATAILFGPAASAQQSPAQGSTEAPTSTSKPAT
                     SATPKSSTAGQSTGAAKTGQAGATAKKPAGAAGTVTLLKTDKDKRSYAIGLNIGAKVA
                     NELKSGGVDMDSAILARGIRDSLTGGKHLMTDEEVKTALTALETELREKARVEFEAMA
                     SANKKEGDDFLATNKSKEGVQTLPSGLQYKVLKAGTGPKPAATDSVSCNYKGTFLNGK
                     EFDSSEKHGGKPVTFVVSEVIKGWTEALLLMPVGSKWQLFVPSDLAYGPRGAGQEIGP
                     NTALIFEVELVSITPKTEEAKPDGKPGTRPEASKPEAKPESKPNSN"
     gene            1397..2695
                     /locus_tag="VFR42_06530"
     CDS             1397..2695
                     /locus_tag="VFR42_06530"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU0368844.1"
                     /translation="MKSVVVVGAQWGDEGKGKVVDYLAGSFDYIVRVAGGHNAGHTVI
                     IGKDKHVLQLIPCGILRPKQHAVIGMGVVVDPVALVKEIEMLAKSGIDVTGRLHISNR
                     AHLIFPYHRELDRAAESARGANKIGTTSRGIGPAYEDKMARRGLRMCDLLDPALFREK
                     AARVVAEKNRLAKGAYGADIDFSDEVDETLKLGEKIRGYICDSAELVNRALGEGKSVL
                     FEGAQGTMLDIDHGTYPYVTSSSATSGGATTGAGVPPTKIKHVLGISKAYTTRVGSGP
                     FPTEMPDLEAQEVRERGKEFGAVTGRPRRCGWLDLAVLRYAKMINGIDSLVITKLDVF
                     DTQREIQVCTGYKYKGQPIYEMPALAEEYEDVVPEYKTLPGWCQDTYGVKDAAKLPKS
                     AIDYLRFISDFLQIEIGMISTGPEREATIIPEATLLSRWL"
BASE COUNT          519 a          917 c          708 g          612 t
ORIGIN      
        1 gtcggaggca aaccaaaaca gaatcgctgg acggcgcgca cgggagtgcc ggcgaaaact
       61 ccggagtcgg acgccatgag caaggactta aagaagcgcg gatttcgttt cataggctcg
      121 acgatttgct acgccttcat gcaagccgtc ggcatggtca acgaccacgc cattacctgc
      181 ttccgctaca actcgctgaa ataaacttct ttagttcgaa ttgggcttgc tctcaggctt
      241 cgcttccggt ttgctggcct ctggtctggt tcccggcttg ccgtcgggct tcgcctcttc
      301 ggtctttggc gtgatggata cgagttccac ctcgaaaatc aacgctgtgt tcgggccgat
      361 ctcctgcccg gctcctcgcg gtccatacgc caggtccgag ggaacaaaca actgccattt
      421 cgaacccact ggcatcaaca acagcgcctc cgtccaaccc ttgatgactt cgctcactac
      481 gaacgtcacc ggctttccgc catgcttttc cgaactgtcg aattctttcc cgttcaggaa
      541 tgttcccttg tagttgcacg acacgctgtc cgtcgccgcg ggcttcgggc cggtccccgc
      601 cttcaacact ttgtactgca gtccgctcgg cagcgtctgt accccttctt tcgacttgtt
      661 cgtcgccaga aaatcatcgc cttccttctt gttcgcggac gccatggcct cgaattccac
      721 ccgcgctttt tcgcgcagtt ccgtttccag ggccgtcagt gccgtcttca cttcctcgtc
      781 ggtcatcagg tgcttgccgc cagtcagcga atcgcgaatc cctctcgcca gaatcgcaga
      841 atccatatcc acgccgccag acttcagttc gttggccacc ttcgcgccga tatttaatcc
      901 gatcgcgtag ctgcgcttgt ccttgtccgt ttttagaagc gtcacagttc ctgcggcccc
      961 tgccggcttc ttggccgtcg ccccggcttg accggttttc gccgcgcctg tgctttgtcc
     1021 cgccgtagaa ctcttgggcg tcgcgctcgt cgccggcttg ctcgtgctcg taggcgcctc
     1081 ggtcgatccc tgcgccgggc tttgctgcgc gcttgccgcc gggccaaaga gaattgctgt
     1141 agcggccact accagagtgg aagaaaagtg ttttctcatg actttcattg tcccattagg
     1201 ccttcgattt cgccaaatcc acgtccagcc gctcccgctt gcgttgccgc atcgtccgtc
     1261 gtacgatttt ctttcttctt ttccctgaaa gcccgttcct cttcgtcgca ctgggaacgg
     1321 tgtgtggtca aaaacgagat agcacagacc tcgtttctgt gccgcgcagt gttagcagga
     1381 accagaaagg tcgcggatga aatcagtcgt tgtcgtgggc gcgcagtggg gtgacgaagg
     1441 caaaggcaaa gtagtcgact atctcgccgg ttcgttcgac tacatcgttc gggtcgccgg
     1501 tggccacaat gccggccaca ccgtcatcat tggcaaggac aagcatgttc tccagctcat
     1561 cccttgcgga attcttcgtc ccaagcagca cgccgtcatc ggcatgggtg tcgtcgttga
     1621 tcccgtcgcc ctcgtcaaag aaatcgaaat gctggccaag tccggcatcg acgttaccgg
     1681 tcgcctgcac ataagcaatc gcgcgcactt gatttttccg taccaccgcg aactcgaccg
     1741 agccgctgaa tccgctcgcg gcgccaacaa aatcggcacc acctcgcgcg gcattggtcc
     1801 cgcgtacgaa gacaagatgg ctcgccgcgg cctgcgcatg tgcgatcttc tcgatcccgc
     1861 gcttttccgc gaaaaagccg cccgcgtcgt cgccgagaaa aatcgcctcg ccaagggagc
     1921 ctacggcgcg gacatcgact tctccgacga agtcgacgaa accctcaagc tcggcgaaaa
     1981 aattcgcggc tacatttgcg actccgccga actcgtcaat cgtgcgctcg gtgaagggaa
     2041 gtccgtcctc ttcgaaggcg cgcagggcac catgctcgac atcgaccatg gcacctatcc
     2101 ctacgtcacc agctctagtg ctacctccgg cggagccacc accggcgccg gcgttcctcc
     2161 aactaaaatc aagcatgtcc tcggaatttc caaggcctac accacgcgcg tcggcagcgg
     2221 tccgtttccc accgaaatgc ccgacctcga agcccaggaa gtgcgcgaac gcggcaagga
     2281 attcggcgct gtcaccgggc gcccccgccg ctgcggctgg ctcgatctcg ccgtcctccg
     2341 ctacgccaag atgatcaacg gcatcgactc gttggtcatc accaagctcg acgtctttga
     2401 cacccagcgc gaaatccagg tctgcaccgg ctacaaatac aaaggccagc ccatttacga
     2461 aatgccggcc ctcgctgaag agtacgaaga cgtcgtcccc gaatacaaaa cgcttcccgg
     2521 ctggtgccag gatacttacg gcgtaaagga cgccgccaag ctgcccaagt ccgccataga
     2581 ttatctacgc ttcatcagcg atttcctgca aattgaaatc ggcatgatct ccaccggccc
     2641 cgaacgcgag gccactatca tccccgaagc cacgctcctc tcccgctggc tgtgatgcac
     2701 gtgtaggttg gcttcggtgc atttgtagcg gccaccttca ggtagcccct ttcttg
//
LOCUS       DASTPM010000373         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1224010, whole genome shotgun sequence.
ACCESSION   DASTPM010000373 DASTPM010000000
VERSION     DASTPM010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224010"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFR42_06535"
     CDS             <1..686
                     /locus_tag="VFR42_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0368845.1"
                     /translation="AEKHKVRLAVIFQDRLKPSIQILKRWLDAGLLGKPLLVDARVKW
                     YRPPDYYSNSRWRGTFALDGGGALINQGIHTVDLLLYLLGDVTCVQARTATLLHKIEA
                     EDSAIATLEFANGAIGTLLATTAAYPGYPRRLEITGTEGTVILEHDRIISANLRNPPP
                     DLAAITSGDANQSASSATVTDITGHQSILENFLHSIATNSDPICSGREARRSLALVES
                     IYASARQKV"
     gene            complement(882..1835)
                     /locus_tag="VFR42_06540"
     CDS             complement(882..1835)
                     /locus_tag="VFR42_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0368846.1"
                     /translation="MTKIICGVDVSSTSLEVFVGPSGPAHSFRNTAEGIAELAAFCRQ
                     QSVDLVAMEASGGYEKQAFALLWASGVPVALLNPRAVRDFAKGMGLLEKTDGIDARVI
                     AWFAAVKGSVANAPASAEQQRLKALVTRLRQLTELRAAQRNQRLLVSEATVRELITEL
                     LKVVSSQMRMLEEEIAALLASDPLWQKLDQALRSIKGVADRTVARVMAEMPEIGTLSN
                     KAVSKLAGLAPLAHDSGKREGKRLVRGGRRNVRAILFVVAGVVRRHDPDFAAFHQRLS
                     AAGKPRKVIRIALAHKLLVRLNARARDARKAFADAGVCQSA"
     gene            2162..2485
                     /locus_tag="VFR42_06545"
     CDS             2162..2485
                     /locus_tag="VFR42_06545"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0368847.1"
                     /translation="MGNDLLPGTLDMLILRSLTRAPRHGYGIAQHLKKISQDVLDVGE
                     SSLYPALQRLLLNGWVTSEWGASENNRRARYYKLTPSGRKQLAAENKEFARMIAAIQK
                     VLSCA"
     gene            2476..>3975
                     /locus_tag="VFR42_06550"
     CDS             2476..>3975
                     /locus_tag="VFR42_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368848.1"
                     /translation="MRLIRRILYFFQQRRHTEDLAEEMDFHRQMLEQESGDRYAANRR
                     MGASTLAREDARAVWFPLRFDNLWQDLRVAFRMLRRSPGFSALAILCLTLGIGANAAV
                     FSWIEGILFRPYPSVAHQERLVAIGGTSRDEPRGTPLSWPDFQDLQRICTLCETTFVS
                     KITGTTLSLGDRAERTTGSIVSANYFDAIGVPPMLGRGFLPGEDLGQSAHPVTVISYQ
                     LWQGRFRGDPQIVGKTERLDGVVHTIVGVAPEGFYGTFVGWRMNFWVPVSMEDTFEGG
                     GYKLEDRGARWIEAYARLKPEVTSAQAQQEFAAISGRLEADYPATNRGRGIRLWPLWQ
                     TPFNNAATLLPTLETMLVVVVCVLLIACANVGNLLLVRSFARRHEMTVRVAIGASRSH
                     LWSQLLTEGLVLSLFGATGGLLVAYWCRHALVLLFPARGGVQMYLPGELDWRVLALSA
                     AVCLATTLLMGLVPALQTGKIDLADALKMESAGVVGGRGRAWVRSGLVLV"
BASE COUNT          753 a         1440 c         1015 g          767 t
ORIGIN      
        1 ccgccgaaaa acacaaagta agacttgccg taatttttca ggaccgcttg aaacccagca
       61 ttcagatcct caagcgttgg ctcgatgccg gcctgctcgg caaaccgctg ctcgtcgacg
      121 cccgcgtcaa gtggtatcgc ccgcccgact actacagcaa ttccagatgg cgcggcacct
      181 tcgcgctcga cggcggcggc gcgctcatca accagggcat ccacaccgtc gatcttctgc
      241 tctatctgct cggcgacgta acctgcgtac aagcccgcac cgccaccctg ctccacaaaa
      301 tcgaagccga agactccgcc attgccactc tcgaatttgc caacggcgcc atcggcaccc
      361 tgctcgccac caccgccgcc tatcctggct acccgcgccg cctcgaaatc accggcaccg
      421 aaggcaccgt aatcctcgag cacgatcgca tcatctccgc caatctgcgc aatcccccgc
      481 ccgacctcgc tgccatcacc tccggcgacg ccaaccaaag cgccagctcc gccacagtaa
      541 ccgacatcac cggccaccaa tccatcctcg aaaatttcct ccactccata gccaccaact
      601 ccgacccaat ctgcagcggc cgcgaagccc gccgcagcct agcgttagtc gaatccatct
      661 atgcatccgc ccgccaaaag gtgtaaatgt agcgccgtgc cttcaggccg gcatcttgtt
      721 gtttccatct ccaacaccaa atcccaccac gagcgcaaac ctaccctctc gagcgaagcg
      781 aggcacctgc cttttctgtc ctcccgaccg gagcgggccg gccttctcct gcgcacggtt
      841 tttgtgcgcc ggcccgcgta gcggagcgac tgtctgtttt gtcaagcact ttgacaaacg
      901 ccggcgtccg cgaatgcttt gcgcgcgtca cgcgctctgg cattgagccg caccaggagc
      961 ttatgcgcca aggcaatgcg aattacttta cgaggcttcc ccgcagcact caggcgttga
     1021 tggaaggcag caaaatccgg gtcatgacga cgcactacgc cggccactac aaacaggatg
     1081 gcccgcacgt tgcgacgccc gccgcgcact agacgtttgc cttctctttt cccgctatcg
     1141 tgagccaacg gtgccagacc tgccaatttg gagaccgctt tgttcgacag cgtcccgatt
     1201 tccggcatct ccgccatcac ccgcgccacc gtgcgatcgg ccactccctt gatcgatcgc
     1261 aaggcctggt ccagtttctg ccacagcgga tcactggcca acagcgcagc gatctcctcc
     1321 tccaacatgc gcatctgcga agacaccact ttcagcagct cggtgatcaa ctcccgcacg
     1381 gtcgcttcgc taaccagcaa acgttggttc cgttgcgcgg cccgcaactc ggtgagctgt
     1441 cgcagacgcg taaccagcgc tttcagccgt tgttgttctg cagatgctgg ggcattcgcc
     1501 acgctgcctt tgactgccgc aaaccaggca atcacccgcg cgtcgatgcc atcggttttc
     1561 tccagcaatc ccatgccttt tgcaaaatcg cgcacggcac gagggttgag caaagctacg
     1621 ggcacaccac ttgcccacaa caaagcaaaa gcctgctttt catatccgcc gctggcttcc
     1681 atggctacta ggtcgacgct ctgctgccga caaaaagcag ctagctcggc gatgccttca
     1741 gcggtgttgc ggaaagaatg cgccggcccc gaaggaccga caaaaacctc caacgaagta
     1801 gaagaaacat ccactccaca aattatcttg gtcacggtaa cctgccttgt gcgtgcgatg
     1861 ggaagtctgg cgactgtccg gtcgtgcgtg actgcggcgg cggtcccaaa cgctccacta
     1921 cggttgttca ccggagggac cacgggcgcc gctcgccgcg tgaggggttc gaccaagtcg
     1981 aacccctcac agcgacattc tcaccaaatc tgccgcctcc atccagatac aagggacccc
     2041 ggcaagatcc caaccaagcc aagcaacgcc tggtcgcgca cctcctgtaa gactccctcc
     2101 cctcgtctat cgacaggacc cccttgacac cacccccgcg ctatgcttcc atcgacaatc
     2161 gatgggaaac gatctcctac ccggcacgct cgacatgctc atcctgcgct ccctgacccg
     2221 cgccccgcgc cacggctacg gcatcgccca gcacttaaaa aaaatctccc aggacgttct
     2281 cgacgtcggc gaaagctccc tctaccccgc cctccagcgc ctgctcctca acggttgggt
     2341 cacctccgaa tggggcgcct ccgaaaataa tcgccgcgcc cgttattaca aattgactcc
     2401 ctcgggccgc aagcaactcg ccgccgaaaa taaagaattc gcccgtatga tcgccgccat
     2461 tcaaaaggtc ttgtcatgcg cctgattcgc cgcatcctgt atttcttcca gcagcgtcgc
     2521 cacacggaag acctcgccga agagatggac tttcaccgtc agatgctcga gcaggaatcc
     2581 ggcgaccgct acgccgccaa tcgtcgtatg ggcgcctcca ctctcgctcg cgaagacgcg
     2641 cgcgccgtct ggtttcctct tcgtttcgac aacctgtggc aagaccttcg cgtggctttt
     2701 cgcatgctgc gccgcagccc cggcttttcc gcgctagcca ttctttgcct cacgctggga
     2761 attggcgcca acgccgccgt cttcagttgg atcgaaggaa ttctcttccg tccttatccc
     2821 tccgtcgcgc atcaggagcg cctggtcgcc atcggcggta cctcacgcga tgaaccccgg
     2881 ggcactccgc tctcctggcc cgatttccag gatctccagc gaatctgcac gctctgtgaa
     2941 acaaccttcg tcagcaagat caccggcacc accctcagcc tcggcgatcg tgctgagcgc
     3001 actaccggca gcatcgtctc cgccaactac ttcgacgcca tcggcgttcc ccccatgctc
     3061 ggccgcggct tcctgcccgg cgaagatctt ggccaaagcg cccatcccgt tacggtgatc
     3121 agttaccagc tgtggcaagg ccgcttccgc ggcgatccgc aaatcgtcgg caagacagag
     3181 cgcctcgacg gcgtagtgca cactatcgtg ggcgttgcgc ccgagggctt ttacggcacc
     3241 ttcgttggct ggcgcatgaa tttctgggtg cccgtttcca tggaagacac cttcgagggc
     3301 ggcggctaca aactcgaaga tcgcggtgcg cgctggattg aagcctatgc tcgtctcaag
     3361 ccggaagtca caagcgcgca agcccagcag gaatttgccg caatttccgg ccgcctcgaa
     3421 gccgactatc ctgccaccaa tcgcggacgc ggcattcgcc tctggccctt gtggcaaacg
     3481 ccgttcaaca acgctgccac cctgctcccc accttggaaa ccatgctggt cgtcgtcgtt
     3541 tgcgtcctgc tgattgcctg cgccaacgtc ggcaatttgc tgctcgtccg ctctttcgcg
     3601 cgacgccacg aaatgaccgt gcgcgtcgcc atcggcgcca gccgctcgca cctttggagc
     3661 cagctcctca ccgaaggcct ggtgctctcc ctcttcggcg ccaccggcgg cctgctggtg
     3721 gcgtattggt gccggcatgc gctggttctg ctcttccccg cgcgcggcgg cgtgcagatg
     3781 tatctccccg gcgaactcga ttggcgcgtc ctcgcgctta gcgccgccgt ttgcctcgcc
     3841 accacgctgc tgatgggcct ggtgcccgcg ctgcaaaccg gcaagatcga cctcgcggac
     3901 gcgctaaaaa tggaatccgc cggagtcgtc gggggtcgcg gccgcgcctg ggtgcgctcc
     3961 ggtttggtgc tggtg
//
LOCUS       DASTPM010000374         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_157138, whole genome shotgun sequence.
ACCESSION   DASTPM010000374 DASTPM010000000
VERSION     DASTPM010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157138"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..1106)
                     /locus_tag="VFR42_06555"
     CDS             complement(267..1106)
                     /locus_tag="VFR42_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU0368849.1"
                     /translation="MGVTQREKGQRFRRLHEGACFVIPNPWDEGSARVLAGMGFAALA
                     TSSAASAAAIGKRDGELTREQALTHAHTIVGATELPVSADLENGFGDAPAAVAETVRL
                     AGEVGLVGCTIEDTKGEDTTGDEAKPLYDFELAVERVAAGAEAARALGFPFLLTARTH
                     NLLFAGGQLEETVRRLQAFEKAGADVLFAPGLPDLAAVRTVCGALRNPFNFMVGIEGK
                     SFSVGELTAAGVRRISLATSLYRAAMTGFLDATREVKEKGTFGFLQRNLKSTGEVVDL
                     MGS"
     gene            complement(1659..>3357)
                     /locus_tag="VFR42_06560"
     CDS             complement(1659..>3357)
                     /locus_tag="VFR42_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368850.1"
                     /translation="GRHSIKLGGDFHWYTWPMWGFFQNRGYYQYTNGYTTEFGFNDGS
                     GNGFASLLLSLPAAKQRQAGIPQMNLRNWGTDAFVEDTWKITQTTTLNLGVRYEYSNP
                     LYDKNNTNTNLIFDNGVPSVFVGGQNGYPKGLMYANTHNFAPRIGVARNLPNWGLVVH
                     AAYGVFFTPVDQNTWCNQRHNVPFVFPETQQADNFTPPAALFKTGMNFGTPVLGTGAL
                     AATTVSFTAFDPHSPAQYVQQWNTSVQKSLDANTTLEVGYVGSRGFHLQRSHLINNTL
                     PGPGPLGPRRPFKTLAFVPDTTLPPSSADNAVLQSQTFPVSTINLLETSAQSWYDAGY
                     VNVRRRYSHGLSLLANYTFSKSLTDAPDFRSPMDEAAIPQNNLDLAAEKGPGCDVRHR
                     FALSAVYAIPSYNRTEWLRLITQNWTFSTIYQAQSGMPFTISVFGDTANSGTVLGENP
                     VRANVTGEPVFGPGTRTADMWFNPAAFATPPAFTYGDAGRNSVYGPPMQTMDIALQRA
                     FRLTERLNFQVRGEAFNALNKVNLGTPNRFVNTPQFGTITMAMTPGREMQVSARLSF"
BASE COUNT          665 a         1143 c          849 g          700 t
ORIGIN      
        1 tcattccgag cgaagcagtt ttcattcgca taacgaatgg aagttgcgta gtcgctcaca
       61 gattccgact cccgtcggaa ggaacctagg cctgttttct ctaagctcga ccatcgatca
      121 gacagcctca actctcgctg cttcccgcaa atctatcttt tcgatgcagc gcttcaccac
      181 aagagccaat ttgggaagaa ccacaaacgc atctgccaac tccacaacgg aacttggcag
      241 atcctcctat ccacacacga cgaggatcaa ctccccatca aatccacgac ttctcccgtg
      301 ctcttcaagt tccgctgcaa gaacccaaat gtccccttct ccttcacctc gcgcgtcgca
      361 tccagaaacc ccgtcattgc cgctcgatac aacgacgtcg ccaagctgat tcgccgcact
      421 cccgctgctg tcagttcgcc aaccgaaaat gacttccctt cgatccccac cataaagttg
      481 aatggattcc tcaacgcccc gcacacggtt cgcaccgcgg ccaggtccgg cagccccgga
      541 gcgaacagca cgtccgcgcc cgccttctca aacgcttgca accgccgtac tgtctcttcc
      601 agctgcccgc ctgcgaaaag caaattgtgc gttcgcgcgg tcaacaaaaa cggaaatccc
      661 agcgcgcgtg ccgcctctgc acccgcagcc acccgctcca ccgccaactc aaagtcgtaa
      721 agcggtttcg cctcatctcc ggtcgtgtct tcaccctttg tatcctcaat tgtgcagccc
      781 accagcccca cttcgcccgc cagacgcacc gtttccgcca ccgccgcggg cgcatcgcca
      841 aacccgttct ccaagtctgc cgacactggc aactccgtcg cgcccacaat cgtatgcgcg
      901 tgcgtcaaag cttgctcgcg cgtcagctcg ccatcgcgtt tcccgatcgc cgccgccgaa
      961 gccgcgctcg atgtcgccag cgccgcaaat cccatccccg ccagcactct tgccgacccc
     1021 tcatcccagg gattcggaat cacaaaacac gccccctcat gcagcctccg aaaccgctgc
     1081 cccttctccc tctgtgtcac ccccatcggt actcctctct ggcctgagct cgctaacaga
     1141 atacgcgact cgactctgtc tcccgcccgg gtcgagtgcg atcagtgaga cgcagcatcg
     1201 cgttgcggct gcagccctcg ctgctggatg aattgcggaa gatagtgctg cgcttacctt
     1261 aacccgggtc gcgggctatc gccgctctcc ccgaggggaa gacaacgacg cccaatgatt
     1321 tgctccatca cgcacgcttc ggatcagaca cttacacggt ctcaactttt tccactgtag
     1381 catcgacctg gccgctccga gccgttgcca tcttaccctc gcgcgatttt catgagcttt
     1441 cgcgggctgt aagtcctaaa ggggacactt acggaaaaga ggggtggggg cctatggttg
     1501 gcttggcgcg ctccaagtgg actcttcagg cgcctcttcc tctgaccgac cacgcaagac
     1561 cgatcacgga acagcgacca aagaacaccg aggcgccagc cgggtacgtt cggtatcccc
     1621 gttcgacttt caactttcga ctgtcgactt cttcttcctc agaacgacag ccgcgcgctc
     1681 acctgcatct cgcggcccgg cgtcatggcc atcgtgatcg ttccgaactg cggcgtgttc
     1741 acaaaccggt tcggcgttcc caggtttact ttattgaggg cattgaacgc ttccccgcgt
     1801 acctggaagt tcagccgctc cgtcagccgg aacgcccgct gcagcgcaat atccatcgtc
     1861 tgcatgggcg gcccgtacac cgagttgcgt cccgcatcgc cataggtaaa cgccggcggg
     1921 gtggcgaacg cagcgggatt gaaccacatg tcggcggtgc gcgttcccgg accaaacacc
     1981 ggctcgccgg tcacgttcgc gcgcaccgga ttttcgccca gcaccgttcc tgaattcgcc
     2041 gtatcgccaa aaacggaaat ggtgaacggc attccgctct gcgcctggta aatcgtggag
     2101 aacgtccagt tctgcgtaat cagccgcaac cactccgtcc ggttgtagga aggaatcgcg
     2161 tacaccgcgc tcagcgcaaa gcgatgccgc acatcgcagc caggcccttt ttccgcggcc
     2221 aggtccagat tgttttgcgg gatcgccgct tcgtccattg gcgaacggaa atccggcgca
     2281 tcggtcaagc tcttcgaaaa agtgtaattg gccaaaaggc tcagcccatg cgaatagcgc
     2341 cgccgcacat tcacgtagcc cgcgtcgtac cagctctgcg cgctcgtttc tagcaggttg
     2401 atggtactga ccggaaaggt ttggctctgt aacaccgcgt tatccgcgct gctcggcggc
     2461 agcgtcgtat ccggcacaaa cgccagcgtt ttgaagggac gtcgcggccc cagcgggccc
     2521 ggccccggca gcgtgttatt gatcaggtgc gaccgctgca aatgaaatcc gcgcgagccg
     2581 acgtagccca cttccagcgt ggtgttcgca tcgaggcttt tttgaaccga cgtattccac
     2641 tgctgcacgt attgcgccgg cgaatgcgga tcaaacgccg tgaagctcac cgtcgtcgca
     2701 gccagcgccc ccgtgcccag caccggcgtt ccaaaattca ttccggtttt gaacagtgcc
     2761 gccggcggcg tgaaattgtc cgcctgctgc gtttccggaa aaacaaacgg aacgttgtgc
     2821 cgctggttgc accaggtgtt ctgatccacc ggcgtgaaga acactccgta cgccgcatgt
     2881 accaccaggc cccagttcgg cagatttctt gccactccga tgcgcggcgc aaaattgtgc
     2941 gtattcgcgt acatcagccc tttcggatag ccgttctgcc cgccgacgaa aacggaaggc
     3001 acgccgttgt cgaaaatcaa gttcgtgttc gtgttgtttt tatcgtagag cgggttggaa
     3061 tattcgtagc gcacacccag attcagggtc gtcgtttggg ttatcttcca ggtgtcttcc
     3121 acgaaggcgt ccgtgcccca atttcgcaaa ttcatctgcg gaattcccgc ttgccgctgc
     3181 ttcgccgcag gcaaactcag cagcaagctc gcaaagccgt tgccgctgcc atcgttgaat
     3241 ccgaattctg tcgtgtatcc attcgtgtat tggtagtagc cgcggttctg gaagaatccc
     3301 cacatcggcc acgtatacca gtgaaagtcg ccgccaagct tgatgctgtg ccggccg
//
LOCUS       DASTPM010000375         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_445738, whole genome shotgun sequence.
ACCESSION   DASTPM010000375 DASTPM010000000
VERSION     DASTPM010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445738"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFR42_06565"
     CDS             <1..573
                     /locus_tag="VFR42_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0368851.1"
                     /translation="FCVSNDHTEIGRIQGRQFAALLPRGGSVLYVQGPSENSAAKERT
                     TGMMATKPANIQVTALKGQWTEESATRAVRSWLQLSTSKKAVIDLIAAQDDSMAVGAR
                     KAFQELANAIERDRWLKLPFTGCDGLPKTGQAWVRSGVLAATVFAPPNVGQAMDMLFQ
                     ALAQNKEQPELALTAPSSVPPLAELKPKPA"
     gene            704..1840
                     /locus_tag="VFR42_06570"
     CDS             704..1840
                     /locus_tag="VFR42_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0368852.1"
                     /translation="MTAGSKKNAVDRRAFLSQTGSGLLAATLPVTNSWAASSEPTAPA
                     AARPHSTVPTGAKRKIPIGVFDPVYDHLSLDQMLDKVSALGLEAMEIGTGGYPNNKHC
                     ALDDLIADKSKARAWQKKFEDKGIRVPTLSCHGNPVHPNAQHAQKDAETFRKTVLLAE
                     ILDIKVIVGFSGCPGGTPTDTQPNWITYRWPPEYNQMLQWQWKEKVIPYWKEAAAYAR
                     SHGIKRLAFEMHPNFVVYNPRTLLKLREAVGEEIGANCDLSHLFWQGCDPVEVIHFLG
                     KQGALFHAHMKDTVIYPENAAKYGVLNFASEANELAEASDTFRAVGYGHSASLWKAVV
                     QAYMDVGYEGILSIENEDPILPGEVGVERAAYVLKNVRKEILGE"
     gene            1894..>2356
                     /locus_tag="VFR42_06575"
     CDS             1894..>2356
                     /locus_tag="VFR42_06575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU0368853.1"
                     /translation="MQRREALRIFAAGAVLPALSPQLFAMFRQAQPPPGYTLRTLTGH
                     LNDTVVAMIDQIIPATDTPGAKGARVNEFIDLILTEWATPEERKNFLDGLAGIDRQSL
                     SLYGKNFSDATVEQQAVQLRAIDDAVMTNRSVRPRHGNTVPKPDSQLEGNFW"
BASE COUNT          521 a          622 c          736 g          477 t
ORIGIN      
        1 ttttgcgtca gtaacgacca cacagaaatc ggccgcatcc aaggacgcca gttcgcggct
       61 ctgttgccac gcggcggcag cgtgctctac gtgcaaggcc cttcggaaaa ctccgcggcg
      121 aaggaacgta caacgggaat gatggccacg aagcccgcga atattcaggt gacagcgctg
      181 aaagggcagt ggaccgaaga aagcgccacg cgcgcagttc gttcgtggct acaactttcc
      241 acgtcgaaga aggcagtgat tgatttgatt gccgcgcagg acgattcgat ggcagttggc
      301 gcgcggaagg cgttccagga gctggcaaat gccatcgaac gggaccgctg gctgaagttg
      361 ccgtttaccg ggtgcgatgg gttgcccaag acagggcagg cctgggtacg cagcggagtg
      421 ctggccgcca cggtttttgc tccgccgaat gttggacaag ccatggacat gctgttccag
      481 gcgctcgcgc aaaataagga gcagccagag ctcgcgctga cggcgccgtc gtcggtgcca
      541 ccactggcgg agctgaaacc gaaaccggcc taaaactgag gcccgacgtt gctctagtaa
      601 aaggattttc tactgctatt tcgcggcttg ctccttttgt ggagaagggt ataattccag
      661 cggatcgtaa acgttaacgg taactctggc gggggtactc tggatgactg ccggttctaa
      721 gaagaacgcg gtcgatcgtc gtgcatttct ttcgcagacc ggcagcgggc ttctcgctgc
      781 aactcttccg gtgaccaatt cctgggcagc ttcttctgag ccgacggctc ctgctgccgc
      841 tcgtcctcac tctacggtac cgactggagc aaaaaggaaa attcccatcg gcgtattcga
      901 tccggtttac gaccatttgt cgctcgacca aatgttggat aaggtcagcg cgctcggcct
      961 ggaagcgatg gaaattggca ccggcgggta tccgaataac aagcactgcg cgctggatga
     1021 tttgattgcg gacaagagca aggcgcgagc atggcagaag aaatttgagg acaagggaat
     1081 tcgcgtgccg acgttgagct gccacggcaa tccggtacat ccgaacgcgc agcacgcgca
     1141 aaaggacgcg gagacgtttc gcaagaccgt gttgctggcg gaaattctgg acatcaaggt
     1201 gattgtcgga ttctccggat gtcccggagg gactcccacg gatacgcagc caaactggat
     1261 tacttatcgt tggccgccgg aatacaacca gatgctgcag tggcagtgga aagagaaagt
     1321 gattccctac tggaaggaag cggcggcgta tgcgcggtcg cacggaatca agcggctggc
     1381 gttcgagatg catccgaact tcgtggtgta taacccccgc acgctgctga agctgcgcga
     1441 agcggtgggt gaagagatcg gcgcgaactg tgatttgagc catctttttt ggcaaggctg
     1501 cgatccggta gaagtgatcc attttctggg gaagcagggc gcgctttttc acgcgcacat
     1561 gaaagacacg gtgatttacc cggagaacgc ggcgaaatac ggagtactga attttgcctc
     1621 ggaagccaac gaacttgctg aggcttccga tacgttccgc gcggtcggtt acgggcacag
     1681 cgcgtcgctg tggaaggccg tagtacaggc ctacatggac gttgggtacg aaggaattct
     1741 ttccattgaa aacgaagatc cgattttgcc tggcgaagtt ggcgtggagc gcgccgcata
     1801 cgtcctgaaa aacgtgcgca aggaaatttt gggtgaatag cttgctccac aagcgttcct
     1861 gttcaccggg tttggttttt ctcaggaggg tccatgcaac ggcgtgaagc gctgcgcatt
     1921 tttgccgcgg gcgcggtttt gcctgcttta tctccgcagc tgtttgcgat gttccgccag
     1981 gcgcagccgc cgcccggcta cacgttgcgc acactcaccg gccatttgaa cgacacggtg
     2041 gtggcgatga tcgatcagat cattccggcg acggacacgc ccggcgcgaa aggcgcgcgc
     2101 gtcaatgaat tcatcgacct gattctgacc gaatgggcca cgccggaaga acgaaagaat
     2161 tttctggacg gcctggctgg aatcgacagg cagagcctgt cgctctacgg caagaatttt
     2221 tccgacgcga cagtggagca gcaagcggtg cagttgcgcg cgatcgatga tgcagtgatg
     2281 accaatcgca gtgtgcggcc gcggcacggc aatactgtgc ccaaaccgga cagccagctg
     2341 gaaggcaact tctggg
//
LOCUS       DASTPM010000376         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_70654, whole genome shotgun sequence.
ACCESSION   DASTPM010000376 DASTPM010000000
VERSION     DASTPM010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70654"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFR42_06580"
     CDS             <1..651
                     /locus_tag="VFR42_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368854.1"
                     /translation="ITGFLALTTRTPAPGSDIFDALNQNPDVYTLSLGHFLDLTGNAM
                     AFFRGPLIGTAFAFSLGPFLNWFLRRRGKPVVANWALVAMMIVFIECAHIALGVFYPI
                     LGSKQFAVAIQQRFQPGEFIVCDGEYANASSVRFYTGRPLLILNGRINGLWYGSLFPD
                     APDIFLDDARFNRLWAGAYRVYLVTGDPKKRDSVAAVAPAYLLTKAGGRYVLSNRP"
     gene            complement(751..1119)
                     /locus_tag="VFR42_06585"
     CDS             complement(751..1119)
                     /locus_tag="VFR42_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368855.1"
                     /translation="MLTVLLLFVLICGSTGGEIAISHGAKKTGEPQRMRPRELLLFIG
                     RVMSNGWFLLGIPLMAASFFALLILFSWHPLSFVIPISALSYVVGALGAKYILKEEVN
                     ASRWAGVILVCAGVAMVAAG"
     gene            complement(1113..1556)
                     /locus_tag="VFR42_06590"
     CDS             complement(1113..1556)
                     /locus_tag="VFR42_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368856.1"
                     /translation="MASEKRLRIKTLIMVLAMVVCAVTGDLLLKRGMSEAGAVQFTGH
                     GLGQALLHMTTSGMIWLGILFLLGFMASQMTVMSWADYSYVMPAGAFGYALQTFLAVA
                     VLHETVSAKRWIGVLLICVGVVLVGQTKPRTSEAAESVEKAAASC"
     gene            complement(1572..>2460)
                     /locus_tag="VFR42_06595"
     CDS             complement(1572..>2460)
                     /locus_tag="VFR42_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0368857.1"
                     /translation="FYTTRGCPAMCTFCMWPQTISGHAWRTRSVDNVVQEVKNTLEYF
                     PQMKEIFFDDDTFNIRRDRVIELSQKFKPLKFQWSCTARCHSKYEELKAMADGGARLF
                     IVGFESGDAQILKNIKKGATTEMAREFMKNCKKVGIKVHGDFIIGLPGETKDTINKTI
                     DFAKELDCETIQVSLAHAMPGTELHDQMSKDGFLRVEALADSGGHQLPHIEYPHLSKA
                     EMMDGVNRFYDEYYFRPKVAWRIVREALWDSHERKRLYHEATSFLKLRAERLKWVRQG
                     GDSAKPMVSVPAAPASGND"
BASE COUNT          480 a          869 c          590 g          521 t
ORIGIN      
        1 atcaccggat ttctcgctct cactacccgt accccggctc caggctccga tattttcgac
       61 gcgctcaatc agaatcccga tgtctacacg ctttctttgg gccattttct ggatctaacc
      121 ggcaacgcca tggccttttt ccgtggaccg ctcatcggca ccgccttcgc cttttcgctc
      181 ggccccttcc tgaactggtt cctgcgccgt cgcggtaaac cggtggtcgc gaactgggcg
      241 ctagtggcca tgatgatcgt gttcatcgaa tgcgcgcaca ttgctctcgg cgttttctat
      301 ccgattctcg gctccaagca gtttgccgtc gccatccagc aacgcttcca gcccggcgaa
      361 ttcatcgtgt gcgacggcga atacgccaac gcatcttcgg tccgcttcta caccggccgg
      421 ccactgctca tcctcaacgg ccgcatcaac ggcctctggt acggctcact ctttccggat
      481 gctccggata tttttctcga cgacgcgcgc ttcaacagac tttgggccgg cgcctaccgc
      541 gtgtaccttg taaccggcga ccccaaaaag cgcgactcag tcgccgccgt cgcgccagcc
      601 tatctgctca caaaagccgg cggccgatac gtcctctcca accgcccgta gctcggcatg
      661 cgctggcgca cccgtcgcca tgcgctctat tggccgccta agtcaattcg gtgtgtcaaa
      721 ttgagcttct gtccgctgaa aggcgctgac tcagccagcg gcaaccatcg ccaccccggc
      781 gcagaccagg atcactcccg cccaccgcga ggcgttcact tcttccttca aaatgtattt
      841 cgctccgagc gcaccgacca cgtagctcaa cgccgaaatc ggaatcacga agctcagcgg
      901 gtgccacgaa aatagaatca gcagcgcaaa aaacgacgcg gccatcagtg gaatccccag
      961 caaaaaccat ccattgctca tcacgcgacc gataaacagc agcaactccc gcggacgcat
     1021 ccgctgcggt tcacctgtct tcttcgcccc gtgcgaaatc gctatctcgc cgccagtgct
     1081 cccgcagatc aggacgaaca acaacagcac cgtcagcacg acgccgccgc cttctccacc
     1141 gattcagccg cctcgctggt acgcggcttg gtctgcccaa ctagcacgac acccacgcaa
     1201 atcagtagca cccctatcca tctttttgcg gaaacggtct cgtgcaggac ggcaaccgcc
     1261 agaaacgttt gcagcgcgta cccgaaggcg cccgccggca taacgtagct gtaatccgcc
     1321 cagctcatca ccgtcatctg cgacgccatg aagcccaaaa gaaacagaat ccccagccag
     1381 atcattccac ttgtcgtcat gtgcagcagc gcctgaccga gcccgtgccc ggtaaactgc
     1441 accgcgcccg cttcgctcat cccgcgcttc agcaacaagt ccccggtgac ggcgcacacc
     1501 accatcgcca ggaccatgat cagcgtcttg atccgtagcc gtttttcgct tgccatgagt
     1561 aacttcgggc ttcagtcgtt cccggacgcc ggagcagcgg gcacgcttac catcggtttc
     1621 gccgagtcgc cgccctgccg cacccacttc aagcgctccg cgcgcagttt caggaagctc
     1681 gtcgcctcat gatacaagcg cttccgctcg tggctgtccc acaacgcttc gcgcacgatg
     1741 cgccacgcca ccttcggccg gaaataatac tcgtcgtaaa accggttcac gccgtccatc
     1801 atttccgcct tcgacaaatg cggatactca atgtgcggca gttgatgccc gcccgagtcc
     1861 gccagcgctt ccacacgcag gaaaccgtcc ttggacatct ggtcgtgcaa ctcggtgccc
     1921 ggcattgcgt gcgccagcga aacctgaatc gtctcgcagt ccagttcctt cgcaaaatca
     1981 atcgtcttgt tgatcgtgtc tttggtctcg cccggcagtc cgataatgaa gtcgccgtgt
     2041 actttgatcc ccaccttctt gcagttcttc atgaactcgc gcgccatctc ggtagtcgcg
     2101 cccttcttga tgttcttcag aatctgcgcg tcacccgatt caaatcccac gatgaacagc
     2161 cgcgctccgc cgtcggccat cgccttcagc tcttcgtact tgctgtggca ccgagccgtg
     2221 cacgaccact ggaacttcag cggcttaaat ttctggctca gctcgatcac ccggtcacgc
     2281 cggatgttga acgtgtcgtc gtcgaagaaa atctctttca tctgcgggaa gtattccagc
     2341 gtgttcttca cttcctgcac gacgttgtcc acgctgcgcg tacgccacgc atgtcctgaa
     2401 atcgtctgtg gccacatgca gaaagtacac atcgctgggc atccgcgcgt cgtgtaaaac
//
LOCUS       DASTPM010000377         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1051344, whole genome shotgun sequence.
ACCESSION   DASTPM010000377 DASTPM010000000
VERSION     DASTPM010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051344"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFR42_06600"
     CDS             complement(<1..266)
                     /locus_tag="VFR42_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368858.1"
                     /translation="MDVFGSFALILAFVCALYAFGGGIAAIITRHPLLIKSARQSGMA
                     TCGLIFVATLSLVYLFFSDNYSVAYVVSHSNRDLSTFFKVAALWS"
     gene            complement(335..760)
                     /locus_tag="VFR42_06605"
     CDS             complement(335..760)
                     /locus_tag="VFR42_06605"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU0368859.1"
                     /translation="MKRQAKFGIGIGVIVVSMAFLAWLGYGESKTYYHTLAELDTLKG
                     SARTERMRIGGTVQNGSIHRMTGRVDFVLEGEGKSLPVSYVGSDPLPDTFVDNSQALI
                     EGRPSAGGRFVAEHIQAKCASKYEAVPGGKPAGTQRSSM"
     gene            complement(791..1363)
                     /locus_tag="VFR42_06610"
     CDS             complement(791..1363)
                     /locus_tag="VFR42_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368860.1"
                     /translation="MPSAQLVCDGCGQFADQEHLARRLKRLENMTRYRPIHVQALFLS
                     AASPAADADHLYSAEDEFRGEGLALLRALGMDPAGKSIETVLTDFQRRGFLLAHILEC
                     PDESADAKGAQGLLEKRLAATTVRTRRSLKPKKVILLGRELDAFVKQFAKETVGAELA
                     LAPSGRAFRLEDLEPGSLEAAIRLPPAASL"
     gene            complement(1485..1808)
                     /locus_tag="VFR42_06615"
     CDS             complement(1485..1808)
                     /locus_tag="VFR42_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368861.1"
                     /translation="MSEPFRPYEKLVDITILGKKFQVPERNSLLRCFQFLSPETIPYG
                     RFCWNQDCQYCRVSCQLPDDDESREMLACKFIVMPGMEVNELSQELKWCLRAKLPQDE
                     TVIQP"
     gene            complement(2109..>2841)
                     /locus_tag="VFR42_06620"
     CDS             complement(2109..>2841)
                     /locus_tag="VFR42_06620"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU0368862.1"
                     /translation="LFTDFVELHGDRAFGDDKALIAGLAKFKGRPVAVIGQQKGRDTK
                     QRVVRNFGQPKPEGYRKALRVMQLAAKFGRPILTFVDTPGAYPGIDAEERGQGEAIAR
                     NLREMARLPVPVIVTVTGEGGSGGALAIAVGDRVNILENGFYSVISPEGCASIIWRDS
                     TKAETAAEAMKITAADLKELGIVDEIVKEPEGGAHTDLESAARLLDEVLDRQLMELLQ
                     EPVSGLVSARYDKFRKMGQFFDLQA"
BASE COUNT          631 a          885 c          732 g          593 t
ORIGIN      
        1 gaccacaacg ccgcaacttt gaaaaacgtg gagagatcgc gattgctatg cgaaaccaca
       61 tacgcgaccg aatagttgtc gctaaagaag agataaacca gactgagcgt tgcgacaaaa
      121 atcagcccgc aagtggccat gcccgattgc cgggcactct tgatcaataa aggatggcgg
      181 gtaatgatcg cggcgattcc gccgccgaag gcatacagcg cacaaacaaa cgccaggatc
      241 agcgcaaagg acccgaatac atccatgccg attccgcttt ctccaaaacc atcaaccgat
      301 gtgcttcccc aagcgggatt actgcaatcg gcggctacat cgaactgcgt tgcgtacccg
      361 ccggcttccc gccgggcact gcttcatatt tggacgcgca cttggcttgg atatgctccg
      421 ccacaaaccg cccgccggct gaggggcgtc cttcgatgag cgcctgcgaa ttatccacga
      481 atgtgtccgg caaaggatcg ctgcccacgt aactgaccgg caaagacttg ccctcgcctt
      541 ccagcacaaa atccacgcgc ccggtcatac gatggatgct gccgttctgc acggttcctc
      601 cgatgcgcat gcgttccgtg cgcgccgaac ctttcagcgt gtcgagttcc gccagcgtgt
      661 ggtaataggt tttgctttcg ccgtagccaa gccacgccag aaacgccatg gaaaccacaa
      721 tgacgccaat gccgatgccg aacttggcct gccttttcat gccaagcact ccctgactct
      781 agctaatcta tcacaaggaa gcagcgggag gtaatcgaat tgcagcttcc agcgagcccg
      841 gctctaaatc ctccagccgg aaggcgcgcc cgctcggcgc tagcgccagt tccgcgccaa
      901 ccgtttcctt cgcaaattgt tttacgaaag catcgagttc cctgcccaac aagatcacct
      961 ttttcggttt tagcgaacga cgggtacgta cggtggtggc cgcaagccgt ttttccagca
     1021 acccttgcgc ccctttggcg tctgcggatt cgtcaggaca ttccaatata tgcgccaaca
     1081 ggaagccgcg ccgctgaaag tctgtcagca ccgtctctat ggatttgccg gctgggtcca
     1141 ttccgagcgc acgcaacagc gccagccctt cgccgcgaaa ttcgtcttcg gctgaataca
     1201 ggtgatcggc atcggccgcc gggctggccg cacttaggaa aagtgcttgc acatgaattg
     1261 gcctataacg cgtcatgttc tccagccgct tcaatcgccg cgcaaggtgc tcttgatccg
     1321 cgaactgccc acacccgtca caaaccagct gtgctgatgg cattcaggta tcgtagtgca
     1381 ggcgcagcct gcccgcaatt taaagggatg gcaatgccag agggacgccg aaggcagccg
     1441 catcagctaa ctccaggcta gtttgaagtg tcgccaggac gctgttacgg ttggatgacg
     1501 gtttcatcct gcggcagctt ggcacgcagg caccacttca attcctggct caattcgttc
     1561 acttccatgc ccggcatcac gatgaatttg caggccagca tttcgcgcga ctcgtcatcg
     1621 tcgggcagct ggcagctcac acgacagtac tgacaatcct gattccagca gaagcgcccg
     1681 tatgggatgg tctccgggct caaaaactgg aagcagcgca atagcgaatt ccgctccggg
     1741 acctggaatt tcttcccaag aatggtaatg tccacgagct tttcataagg acgaaagggt
     1801 tccgacataa aaaattccgg tttccgcggg cacgcaacga tgctgggcac aacgctccgg
     1861 gttccagaat ttcaggatgt accttccaac gacggggtga agaatcgtct tgcaaccctg
     1921 actggaaaag cccctgcccg cttgcttgtt ataccaatca agcctagcgc gcacgtttgc
     1981 gagcttacac agtttcgtaa agcgcgcgtt cctatgtgca aagggtgagt actgatccca
     2041 agtgttgcga cggaagaaac acggcggcaa ttcaagaggc aaccatctga tttcggagtg
     2101 cttgggaatc aagcctgcag atcgaaaaac tggcccatct ttcggaactt atcgtagcgc
     2161 gcggaaacca ggccgctcac cggctcctgc aacaactcca tcagctgccg gtccagcact
     2221 tcatccagaa gacgcgctgc agattccagg tcggtatgcg caccgccttc cggctctttt
     2281 acaatttcgt ccacaattcc aagctctttc aggtcggcgg cggtaatctt catagcttcc
     2341 gccgcggtct cggctttggt cgaatcgcgc cagatgatgc tcgcgcagcc ctccggcgaa
     2401 atcacggaat aaaaaccgtt ctctagaatg ttcacgcggt cgccgaccgc gatggccaac
     2461 gctccgcccg agccgccttc tccggttacc gtgacgatta ccggcaccgg cagtcgcgcc
     2521 atctcgcgca agttgcgcgc aattgcttcg ccctggccgc gctcttcggc gtcaattccc
     2581 gggtaagccc ccggtgtgtc cacaaacgtc aaaatcggcc ggccaaactt agccgccagt
     2641 tgcatcacgc gcagcgcttt gcgataaccc tctggctttg gctggccaaa attgcgtacc
     2701 acgcgctgct tggtgtcgcg tcctttttgt tgaccgatca cggccaccgg ccgtccttta
     2761 aatttcgcca agcctgctat caaagccttg tcgtcgccaa atgcgcggtc accatgcagc
     2821 tccacaaaat ccgtaaagag c
//
LOCUS       DASTPM010000378         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1368455, whole genome shotgun sequence.
ACCESSION   DASTPM010000378 DASTPM010000000
VERSION     DASTPM010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368455"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..761)
                     /locus_tag="VFR42_06625"
     CDS             complement(24..761)
                     /locus_tag="VFR42_06625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0368863.1"
                     /translation="MRTLIAKALRKAAHAIGPDSEPIDITDHYVKWLCYANAGMLNRG
                     NLYLMDYALKRIPSQAPIIEIGSFCGLSANLLTYYKKKYSLSNKLITCDKWEFEVPKG
                     EPSDYVAGSSVRFTEYMALVKNSFLRNTQTFSADDLPFTIEAFSQEFFSSWRKNMPVH
                     DVFGRPIALGGPISFCYIDGNHSYEGVKFDFQNCDEFLEVGGFILFDDSFAKDFGVYK
                     LMPEIKRMDRYQLAAKNPNHLFQKIRK"
     gene            1080..1628
                     /locus_tag="VFR42_06630"
     CDS             1080..1628
                     /locus_tag="VFR42_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0368864.1"
                     /translation="MTGKMVTPGRPQPGEYSQYYEKYIALVASTDILGALEAQRLVMS
                     QLLGARSEREGNFRYAPDKWTVKEVIGHLADSERIFAYRALRFARGDQTPLSGFEQDD
                     YVKGGGFSERTLADLAEEFAEVRGASIALFSGLDAAAWQKRGVANKNEITVRALAYII
                     AGHDLHHRRVLEEKYLPAIPRA"
     gene            1854..>2150
                     /locus_tag="VFR42_06635"
     CDS             1854..>2150
                     /locus_tag="VFR42_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368865.1"
                     /translation="MSCGEGVGRAATDYPRLTFGGNGLCQGGLFARAEKILLTKCRRG
                     LALFERRPAGESNSAWWRRLFAGGMPDNPDWCGAKVRWETWLVSSYRHGNRQEVH"
BASE COUNT          509 a          528 c          679 g          434 t
ORIGIN      
        1 cggtggcgct ttgtatgctc ctgctacttg cgaattttct gaaagaggtg gtttgggttt
       61 tttgcggcca gttggtagcg gtccattctt ttgatttcag gcatcaattt gtaaacgccg
      121 aaatcctttg cgaaggaatc atcaaagaga atgaagccgc cgacctccaa gaactcgtcg
      181 cagttctgaa agtcgaattt cacgccttca taagaatggt tgccatctat gtagcagaaa
      241 ctgatcggcc cccccagtgc tatcgggcgc ccgaagacgt catggaccgg catgtttttc
      301 cgccaggacg agaaaaactc ctgggaaaag gcttcgatag tgaaaggcag atcatccgca
      361 ctgaatgttt gggtattccg caagaaagag tttttaacca gcgccatgta ttcggtgaaa
      421 cgaaccgacg atccggcaac atagtccgag ggctcgccct tgggaacttc gaattcccat
      481 ttatcgcagg tgatgagctt gttgctgagc gaatattttt tcttgtaata ggtcaagaga
      541 ttcgcggaga ggccgcagaa ggagccgatt tcgatgatcg gagcctggct gggtatcctt
      601 tttaacgcat agtccattaa atagaggttg ccgcgattca gcatcccggc attcgcgtag
      661 caaagccact tgacgtagtg gtcggtaatg tcgataggtt cggaatccgg tccaattgca
      721 tgagcggcct tacgtagagc cttggcaatc agcgttcgca tcgtgcgtag aaaaatctcc
      781 gtaagaaatc gtaaatgaag caccaagtga gtgcaacgtc cggagaaaac cgtggcccaa
      841 cgcgttcaga cagacagcct gcgtcagtat gttatataag tggttacttg ccgagagttg
      901 gcgaaattcc aacatcatcg ggtaacggct cctggatcgg cttgccgaag agaagatctt
      961 gaggcttaca ctgtacgccc gcatacaggc tcgtatgcgg aaggccgcta tgctgtcgca
     1021 tgccgggtca gatgccgagc cgggagacgc gagcgcgacg gtggagctat aatgaggtca
     1081 tgacaggaaa gatggtgacg cccgggcgtc cacaacccgg tgaatatagc cagtattacg
     1141 agaaatatat tgcgctggtg gccagtacgg acattttggg agcgctggag gcgcagcggc
     1201 tggtcatgtc gcaactgctg ggcgcgcgca gcgaacgcga aggaaatttc cgctacgctc
     1261 ccgacaagtg gacggtgaaa gaagtgatcg gacatttggc agattcggaa cgaatttttg
     1321 cctaccgggc gttgcgattt gcccgaggcg accagacccc gctttccgga tttgagcagg
     1381 acgactacgt gaagggcggg ggatttagcg agcgcacgct ggcggacctg gcggaagagt
     1441 tcgccgaagt gcgcggagcg agcatcgcgt tgttcagcgg actggatgca gccgcgtggc
     1501 agaaacgcgg cgtggcgaac aagaacgaaa tcacggtacg cgcactggcg tacattatcg
     1561 cggggcacga tttgcatcac cggcgggtcc tggaagagaa gtacctgccg gcgattccgc
     1621 gggcctgatg cggttggtta ggtcgaaggt gcaagatcag gacgatcagc aagagtgaaa
     1681 aggtcaggca aaccgagccc atgatgacct gcccggaatc agccgcgaat ggatgcgcga
     1741 aggcgtcgta gagaattcca ggccccaaca gcaagaactg cacgcagacg agcaaaatta
     1801 gcgagccgaa cgtgtgcacg acaccggcgc ggaagcgcca ggagcgcgca cgggtgtcct
     1861 gcggcgaagg cgtgggaagg gcggccacgg attacccgcg gctcaccttc gggggcaacg
     1921 ggctgtgcca gggcgggctg ttcgccaggg cggaaaaaat cctgctaacg aaatgccggc
     1981 gaggcctggc gctttttgag cggcgacccg caggcgagag caattccgcc tggtggcgga
     2041 ggcttttcgc cggggggatg cctgacaatc ccgattggtg cggtgccaaa gttcgctggg
     2101 aaacatggct ggtgagttcg taccggcatg gtaatagaca ggaggttcac
//
LOCUS       DASTPM010000379         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1079940, whole genome shotgun sequence.
ACCESSION   DASTPM010000379 DASTPM010000000
VERSION     DASTPM010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079940"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFR42_06640"
     CDS             <1..1248
                     /locus_tag="VFR42_06640"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU0368866.1"
                     /translation="VAAVLAVLLAGSSTPAYIFQQRVLASTEWNRQVELANADAARIR
                     QSIVVPTDQRVRAGENFAAALQKLGLTPQEAADATSAAQQAFNLRQLRAGNTLTVGRS
                     VEGQLREIDYRIDVDRMLKILPQEQGFSAKVADIPSRTEIVAVRGILDDSLFNAVEET
                     GESAELAVRMTQIFGYDLDFYTDPRRGDTFCILLEKRRYANGQTAGYGKIFAAEYNNA
                     GKKYQALLYHDPAGQPGYYTEDGKSLQKAFLRSPLKFGAPVTSHFSNARFHPILKTYR
                     AHLGTDYGAPVGTPVQTIGAGRVTFAGRKGGEGNMVQISHTNGYETMYLHLSRMFVRL
                     GQHVELGKTIGLVGSTGLSTGPHLDFRILQKGQYRNFERLGLPPSDPVAKKNFPEFAA
                     LRERWLPLLKNPDLLQVATVVAR"
     gene            complement(1263..2684)
                     /locus_tag="VFR42_06645"
     CDS             complement(1263..2684)
                     /locus_tag="VFR42_06645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEU0368867.1"
                     /translation="MSAPGFTTLPHLEHPFGHMDELAKVAALPLTGEADPVPQSLLVS
                     FPEQAKTLGLLYDVSRELTSILDREELLRRITELVKKLVNYDVFTVMLWNEKTQMLEG
                     VFAKHYEDSIPSRFRVSLNQGVTGHAAAARKPIRLDDVRLDSRYIDCETCERVHSELV
                     VPLLLRDRLIGVLDLESAQPAAFTAQHERMLVALGPFIAIALENSRLYQDARENELRL
                     QNDLDTAREIQLQLLPRGAREVPGLDISAAYVPARELGGDFYDFLPYGEGRLALALGD
                     VSGKGTAAALYGSLAIGMLREHAVENTAAPPVMLAMLNRRLHAARLEARFIALTFGVY
                     DPMERTLTMANAGGTHPLLLRGGELSEIPVDGIPLGLFPEAEYSSSTLHLRVGDVVVF
                     ASDGILESENAEQEEFGFERLRALLTSLPADSPVDDISGAILRATDEFAGFGNPAHDD
                     RTLLVIRVTPDSPSDLPKMPVIY"
     gene            3242..>3773
                     /locus_tag="VFR42_06650"
     CDS             3242..>3773
                     /locus_tag="VFR42_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0368868.1"
                     /translation="MKRLMAPMALVAMLLIARCVSAEELFDIKPVADGVYAAISKPAY
                     KVNCNAVIIFLGDSVLVVDTHSKPSAARALIAQIKKLTDKPVRYVVNTHFHWDHYQGN
                     EAYPSSWPAGIEIISSEATRNNIQRLGIPRVKHEIITMPAEIDQLKADLAKASTQQQK
                     ETIGNNLKEAEAYFAEL"
BASE COUNT          865 a         1101 c         1066 g          741 t
ORIGIN      
        1 gtggcagcgg ttttggcggt gctgttggcg ggtagttcca cgccggctta tatattccag
       61 cagcgcgttc tggcctcgac agaatggaat cggcaggtag aactggccaa tgcggacgcg
      121 gcacgtatcc ggcagagcat cgtcgttccc accgatcagc gtgtgcgcgc gggagaaaat
      181 ttcgctgctg cgttgcagaa actcggactc actccgcaag aagccgcgga cgctacatcc
      241 gcggcgcagc aagccttcaa tctccggcaa ctccgcgccg gaaacacgct aaccgtgggt
      301 cgctccgtcg aagggcaact ccgcgaaatt gattaccgca ttgacgtcga ccgcatgctc
      361 aaaattcttc cgcaagagca aggcttctcc gccaaggtag cggacattcc gtcgcgcacg
      421 gaaattgtcg cggtgcgcgg catactcgac gattcgctct ttaacgccgt cgaagaaacc
      481 ggcgaatccg ccgaattggc tgtgcgcatg acgcaaatct tcggctacga tctggatttt
      541 tacaccgacc cgcgccgtgg tgatactttc tgcatcctgt tggagaaaag gcgttacgcc
      601 aacgggcaaa ccgccggtta cggcaaaatc ttcgcggccg aatacaacaa cgctggaaaa
      661 aaatatcagg ccctgctgta tcacgatccc gcgggccagc ccggttatta caccgaagac
      721 ggcaagtctc tgcagaaagc gtttctgcgc tctccgctga aattcggagc gccggtcact
      781 tcgcatttca gtaatgcgcg gttccatccc attctgaaaa cctatcgtgc gcacctgggc
      841 acagactatg gcgcgccggt gggaactccg gtgcagacca ttggcgcggg ccgtgtaacc
      901 ttcgcgggac gcaagggcgg agaaggcaac atggtgcaga tttcgcatac caacggctac
      961 gaaaccatgt acctgcactt gtccaggatg tttgtgcgtc ttggccagca cgtggagctt
     1021 ggcaaaacga taggactcgt cggcagcacg ggactttcaa ccggcccgca tctggatttt
     1081 cggattctgc aaaaagggca gtacagaaat ttcgagcgcc ttggcttgcc gccttccgat
     1141 cccgtagcca aaaagaattt tccggaattc gccgccctgc gcgaaaggtg gctgccacta
     1201 ctgaagaatc ctgacctgct tcaggtcgca accgtagtcg ctcgctgaaa ccgagcccgt
     1261 cctcaataaa tcacaggcat cttgggaagg tcggacgggg agtccggcgt gacgcggata
     1321 accagcagcg tgcggtcatc gtgcgcggga ttgccgaagc cggcgaattc gtcggtggcg
     1381 cggagaattg cgccagaaat gtcgtccacg ggactgtcgg cgggcagcga ggtaagcagc
     1441 gcccgcagcc gctcgaaacc gaactcttct tgctcggcgt tttcggactc gagaatgccg
     1501 tcggaggcga acacgaccac gtcaccgacg cggagatgaa gcgtcgatga agagtattcc
     1561 gcctccggaa aaagcccaag gggaatcccg tccaccggaa tttccgaaag ctcgccgcct
     1621 cgcagcagca acggatgcgt gccgccggca tttgccatcg ttagcgtgcg ctccatcggg
     1681 tcatagacgc caaatgtcag cgcaataaag cgcgcttcga gccttgcggc gtgcagccgc
     1741 cggttcagca tcgccagcat caccggcggc gctgctgtgt tttctacggc gtgttcgcgc
     1801 aacataccga ttgccagcga accataaaga gcggccgcgg ttccttttcc ggaaacgtcg
     1861 cccagtgcca gcgccaaacg tccctcgccg taaggaagaa agtcgtaaaa gtcgccgcca
     1921 agttcacgtg ccggcacata tgcggcggaa atatccaagc cgggcacttc gcgcgccccg
     1981 cgcggcaaga gctgaagctg aatttcccgc gcggtatcga gatcgttctg caggcgcaat
     2041 tcattttcgc gggcatcctg atacagccgc gaattttcca aggcgatggc aatgaagggc
     2101 cccagcgcca cgagcatgcg ctcgtgctgc gcggtaaatg ctgccggctg cgcactttcc
     2161 agatccagca caccgatgag ccggtcccgc aaaagcaacg gaaccaccag ttccgaatga
     2221 accctttcgc aggtttcgca atcgatgtat cgcgaatcca gccgcacgtc gtccaggcga
     2281 atcggcttgc gggccgctgc ggcatgcccg gtcacgccct ggttcaatga aacccggaag
     2341 cgcgacggaa tggaatcctc atagtgcttg gcgaaaacgc cttccagcat ttgtgtcttc
     2401 tcgttccaca gcatgaccgt gaaaacgtca tagttgacga gcttcttcac gagttcggtg
     2461 atgcgacgaa gcaactcttc gcgatccaga atcgaggtca gctcgcggct gacgtcgtaa
     2521 agcaggccca gggttttggc ttgttccgga aaagacacca gcaacgactg cggaacggga
     2581 tcggcttctc ccgtcagggg cagagcggcg actttggcaa gctcatccat gtgaccgaaa
     2641 ggatgctcca agtgcggtaa agttgtgaat ccgggtgcag acatagaata tgccttaatt
     2701 caattggatt cacgagagcc gaaatggttt caccgtgcga tggttggagg cgatggcaat
     2761 tccggaggtt cgggaggaga aggaagacga attcctccat ggtgcgagcg tgggccgttg
     2821 ctgggcgcca gcacgatgtc gacgccatcg ggccggtaaa acgcagttgc gccatcgcgc
     2881 cgcgccataa accgataatt ttctggtgct tggccctgat tttcctcgaa aacctgcggc
     2941 ataatgagcc gaaccggaga ccatgtttgc ccgcgggacg acggagcatg attcaacaac
     3001 acaacgggca acgaacacag ctggagtcga ccaaggctgg gcaagggcat tcgcaggaag
     3061 gcccttgaat aagttgtctg cggactaact tactttacgc gtatttcggt gatggcaaaa
     3121 gaagtttatt gcgatccacc agttcgcgca agcacatcca cgattatcgt ttctcattac
     3181 cgaaagccgg cggctgtggt aggctcctcg ccaaaatggc gaccccctcc ggaggcacta
     3241 tatgaaacgc ttgatggcgc caatggcgct tgtggctatg ctgttgattg cgaggtgtgt
     3301 ctcggcggag gaattgttcg acatcaaacc ggtcgccgac ggcgtctacg ccgcaatttc
     3361 caaacccgct tacaaagtga actgcaatgc cgtcatcatt tttctcggcg acagcgtcct
     3421 tgtagtggat acgcactcca agccttccgc tgcccgggcc ctcatcgcgc aaattaagaa
     3481 actcaccgac aagccggtgc gctacgtcgt gaacacacac tttcactggg atcactatca
     3541 aggcaatgag gcttatccca gctcttggcc cgccggcatc gagatcatct cgtcggaagc
     3601 cacccgtaac aacatccagc gcctcggaat tcctcgcgtc aaacatgaaa tcatcaccat
     3661 gcccgcggaa attgatcaac taaaagcgga tttagccaag gcttcgacgc agcaacaaaa
     3721 agaaaccatc ggtaacaatc ttaaggaagc ggaagcctat ttcgcggaac tca
//
LOCUS       DASTPM010000380         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_935965, whole genome shotgun sequence.
ACCESSION   DASTPM010000380 DASTPM010000000
VERSION     DASTPM010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935965"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="aroC"
                     /locus_tag="VFR42_06655"
     CDS             complement(<1..832)
                     /gene="aroC"
                     /locus_tag="VFR42_06655"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU0368869.1"
                     /translation="MPDSLPIITHRIQSEYMLKFFTSGESHGEALVAFVSGLPAGLEV
                     AQEFVDHELWRRQQGYGRGGRMKIERDSARILSGVRHGQTIGSPISMLLENRDWKNWQ
                     QQLPVEKGDSSLHKRVASPRPGHADLAGALKYNFSEARYVLERASARESAARVAVGAL
                     AKLFLKQLNIEVLSHVVAVGAANVEHEVVWEKIKASCDREEVLLNCAEPEAEQIMKGE
                     VDKVLRTGDSVGGVFEVVAHGVPAGLGTYAQWDERLDAQLAAAVMSLQAVKAVEIGSG
                     V"
     gene            1017..1778
                     /locus_tag="VFR42_06660"
     CDS             1017..1778
                     /locus_tag="VFR42_06660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU0368870.1"
                     /translation="MSHKAAPRRQVADATASDAAEVSRHLLKGEEEERRRISRELHDE
                     TGQALMVLRFQLEMLASEAKGPGQKAKVQESLDVLDRTIEGLRRIIARLSPRVLEEFG
                     LLAAIRRHAQLLASQTRMKARVELPEEVAAMDHDIEVALYRSAQEALHNIAKHSRARN
                     FVVRLRTVGSEVHLEIEDDGTGVLTKAPDDGGYGLTGMRERAVALGGSMTIQSRRERG
                     TQIRIVLPCSERESSTAENKRGARVIRRASTAKAG"
     gene            1786..>2214
                     /locus_tag="VFR42_06665"
     CDS             1786..>2214
                     /locus_tag="VFR42_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0368871.1"
                     /translation="MPIRCLIVDDHTLFREGLRRVLESEPDLQVVGEARDGSEAIEQA
                     QALKPDVILLDIGMPGLSSFEASRRITRELPGRRVIFLTMYEDEEYLLQCLDAGASGY
                     ILKDAPAPRLIGAVRDVNAGRKYLSPQVLGKLVDDFRSRST"
BASE COUNT          456 a          589 c          713 g          456 t
ORIGIN      
        1 ctacgcccga cccgatttca actgctttga cggcttgcag cgacattacg gctgcggcga
       61 gttgcgcgtc gagacgttcg tcccattgag cgtaggtgcc gaggccggcg gggacgccgt
      121 gtgccaccac ttcgaagacg ccgccgacgg agtcgccggt tcgcagaact ttatcgactt
      181 cgcccttcat gatttgctcg gcttctggtt cggcgcagtt cagcaacact tcttcgcgat
      241 cacaactggc tttgattttt tcccaaacga cttcgtgttc cacgttggca gcgccaaccg
      301 ctaccacatg gctcaggact tcaatattca gctgcttcaa aaataatttg gctagagctc
      361 cgacggcgac ccgggcggcg gattcgcggg ccgaagcgcg ctccagaaca tatcgggctt
      421 cagaaaagtt gtatttcagc gcaccggcga ggtcggcatg tccgggacga ggagacgcca
      481 cccgcttatg cagactagaa tcaccctttt caaccggaag ttgctgctgc cagtttttcc
      541 agtcgcgatt ttcgaggagc atggagatgg gagagccaat agtttgtcca tgacgtacac
      601 cggatagaat gcgcgcggaa tcgcgctcga ttttcatcct gccaccgcgg ccgtagccct
      661 gctggcggcg ccacagttcg tggtccacga attcctgcgc gacttcgagg cccgcgggga
      721 gtccggagac aaatgcgacc agggcttcgc cgtgagactc tccagaggtg aagaatttca
      781 gcatgtactc ggattgtatc cgatgagtaa tgatggggag gctgtcgggc aaacccgcaa
      841 ctccgcttca catccaagac ttggctacgc gtgttttgcg tcgacgggag tagtgtgaag
      901 gcggagctcg ccgacggttc gggatgacgt tctgtaagcc gagctttgct cgaccggaca
      961 gccgagggcg gctgtcccca catggcctgt ggcttttgta gttttgaggt gattgggtgt
     1021 cccataaagc tgccccaagg cggcaagttg ctgatgcgac cgcttcggat gcggcggagg
     1081 tttcgcggca tctgttgaaa ggcgaagaag aggagcggcg tcgtatcagc cgcgagttgc
     1141 atgatgagac agggcaggcg ctcatggtcc tgcggtttca acttgagatg cttgcgtcag
     1201 aggctaaggg tccgggacag aaagctaagg tccaggaatc gctcgatgta ctggatcgaa
     1261 ccatagaagg tttgcggcga atcatcgcca ggttgtcgcc gcgcgttttg gaagagtttg
     1321 gactacttgc cgcaattcgg cggcatgcac aactgctggc cagccagacc cgcatgaaag
     1381 cccgcgtgga gctgccggaa gaagttgcgg ccatggacca cgacattgaa gtggcgctct
     1441 atcgctcggc gcaagaagcc ttgcacaaca ttgcgaaaca ctcgcgcgcg cgaaatttcg
     1501 tggtgaggct gcgcacggtg gggagcgagg tccaccttga aatagaagat gacggaaccg
     1561 gagttttgac gaaggcgccg gacgacggag ggtacgggct caccggaatg cgcgaacgcg
     1621 cggttgcgct cggtggttcg atgaccatcc aatcgcggcg cgaacgcggg acgcagattc
     1681 gaattgtttt gccttgtagc gagcgtgaat cttctactgc agagaacaaa cgaggcgccc
     1741 gggtgatccg gcgcgcctcg acagcaaagg ccggttaagg aagctatgcc gattcgatgt
     1801 ctcattgtgg acgaccatac gctgttccgc gaggggctgc ggcgggtgct cgagagcgaa
     1861 cccgatctgc aagttgtcgg agaggcgcgg gacggatcgg aagcgatcga acaggcacaa
     1921 gcgctcaagc cggatgtgat ccttctggat attggaatgc cggggttgtc gagcttcgag
     1981 gcgtcgcgcc ggatcacgcg ggaattgcca ggacggcgcg tgatctttct taccatgtat
     2041 gaagatgaag aatatctgct gcagtgcctg gacgccgggg cctcgggata cattttgaaa
     2101 gatgcgccgg ccccgcgatt gatcggcgca gtgcgcgacg tgaatgcggg caggaaatat
     2161 ttaagtccac aggtgctggg gaagctggtg gacgatttcc gctcgcggtc aacc
//
LOCUS       DASTPM010000381         4972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_474794, whole genome shotgun sequence.
ACCESSION   DASTPM010000381 DASTPM010000000
VERSION     DASTPM010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474794"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="VFR42_06670"
     CDS             <1..1984
                     /locus_tag="VFR42_06670"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0368872.1"
                     /translation="GETYTKNATKVALTNGMTILDARQDGKVLPLELENGMTTAVLPG
                     SASFAVALDAGLPLGIEAGRASFTIPVPAAGSARLSLIVPGDHTNVRISPGLITSRSS
                     ANGQTSIEATLVPGQAASLWWTTREVATPTVPREARFLSNLKTLFTVSESDLRIAALA
                     DITVVQGDPSQFTLTVPAGYEVTSVTGPTLESSETEDNKLILNVTNPAQRNHEFLISL
                     EKSVTDSKADAPFLSFSHSQRETGEALVEGSGTMELTAKESASLKRMDVKEVNPYLRA
                     LARFPMQAAFRYHRQPSEPPSLALAWTRFPDSSVLAAVAERAVVTTLVTVEGKSLTEV
                     KLTVKNQAQPFLKVGLPSGATIVSADVAGEKVKPVQGSDGARVPLLRPGFRPNGSYAV
                     SFVFLHSGAPFAKKGGSDLSLPKMDVPIDLLQWEVFLPQQYKVKDFGGDALATSLLPP
                     TSWDSAEEERGFGWGVGHGYAPPVNERDFKMPSLAPGQIGGIVVDPTGAVIPSAKVTV
                     VHQDTGTTLTTTTDDSGRWVVSNVPSGRVHVTAASPGFKDYRQDANYDASRPLPLSFN
                     MNLGAATETVTVMAGVSGLQTESAEISSRIEKQARKRALEQERAASSNVTNLQQRVAG
                     VLPVAVDVPHAGNSYRFVRPLVLDEETKVTFTYKTK"
     gene            complement(2026..3744)
                     /locus_tag="VFR42_06675"
     CDS             complement(2026..3744)
                     /locus_tag="VFR42_06675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU0368873.1"
                     /translation="MQVPRSVPFGVPRRTEPEAAQRLADGEARAQLILDTAPDAFVGI
                     DSEKRIVIWNLQAAATFGWNADEAIGQTLFETIFPEQDRQANCGGVEGFFPRDQAAVM
                     DHRLELCARHRDGRLIPVELTINGPLDGKGGQLFGAFVRDISQRKEREQELQQARQSA
                     EARARTLEILNEISRELSALLNTDELLKRIGELLSRLLEYQTFSILLLDASGKTLKHR
                     FSLSGGQVIEKPDIPLDRGLVGYAARYRHPVVVPDVREDSRYIKFHDETRSELSVPLI
                     AKDKLVGVLDIENATPDYFREGHIQAITILASQLAVALDNAILYDRISSQEAQLNQDL
                     RIARELQKRLLPSGGPAMKNAAVSTMSCAARIIAGDMVHLGYYRKQGLHVGMLGDVAG
                     KGAAAALYAALTMGLIRSLTEHELSPGEMLKALNQSLMERAMEDRFVALCYTVWDDAT
                     LNFRIANSGLPKPVLFRRGKVEILEAVGMPLGLFGGVEFEEMRIAAEPGDVFLFLTDG
                     ILEAGNRADDEFGYEGVERALRATRTNGAREIREVLAETLRAHCGDVEARDDQTLLVL
                     KVNEQV"
     gene            3878..4804
                     /locus_tag="VFR42_06680"
     CDS             3878..4804
                     /locus_tag="VFR42_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU0368874.1"
                     /translation="MKTTSACLALLFCFSATCHAQSSTIPTSQDDSMVLIHGATFQMG
                     IDASEIPRFQKIFNIDHPQLFNDELPKHSVTVADFYLDKHLITNAQFKNFLLARPSWR
                     PTQLPPDLDNGHYAQRWADPHQPNLKPDHPAVNVSWYVATAFCGWAGKRLPTEAEWEF
                     AARGGQAGVLFPWGDAPPDQSRANFADNVGTTTPVEAYPPNAFGLYDMAGNAWQFLSD
                     EWTPYPSHSGDTVPLHVIQKEPKDLVLHRDDRRHVIRGGSYAGAPINLWVEYRDSHPA
                     NGSQPFVGFRCAKSASAPGKKQEVDRRGAPKI"
BASE COUNT         1010 a         1814 c         1178 g          970 t
ORIGIN      
        1 cggcgaaacc tataccaaaa acgccacgaa agtggctttg accaatggca tgaccatcct
       61 cgacgcgcgc caggacggca aagtactccc tcttgagctc gaaaacggca tgaccaccgc
      121 agtccttccc ggttccgcca gcttcgccgt cgctcttgat gccggccttc cgcttggtat
      181 cgaagccggc cgcgcctctt tcacgattcc cgttccagcc gcgggcagcg cgcgcctttc
      241 tctcatcgtc cccggcgatc acacgaacgt gcgcatctcg ccgggcctca tcaccagccg
      301 ctcctccgcc aacggccaaa cttccatcga agccacgctc gttcccggcc aagccgcttc
      361 cctgtggtgg accactcgcg aagtcgccac tcccaccgtt ccgcgcgaag cccgcttcct
      421 ctccaacctc aaaactcttt ttaccgtcag cgaatccgat ttgcgcatcg cggctctcgc
      481 tgacatcact gtcgtgcaag gtgatccatc tcaattcact ctcaccgtcc ccgccggcta
      541 cgaagtcacc agcgtcaccg gccccacgct cgaatccagt gaaaccgaag acaacaagct
      601 catcctcaac gtcaccaacc cagcacaacg caaccatgaa tttctcatct cactcgaaaa
      661 gtccgtaacc gactccaaag ccgacgctcc cttcctctcc ttcagccatt cccagcgcga
      721 aaccggcgaa gcgctcgtcg aaggctccgg caccatggaa ctcaccgcaa aagaatcggc
      781 cagcctgaag cgcatggacg tgaaagaagt gaacccgtat ctccgcgccc tcgcgcgctt
      841 cccaatgcaa gccgcattcc gctaccaccg ccagcccagc gaaccgccat ctctcgctct
      901 cgcctggacc cgcttcccgg acagcagcgt cctggccgcc gttgccgagc gtgccgtggt
      961 caccaccctc gtcaccgtcg aaggcaaatc cctcaccgaa gtcaaactca ccgtcaaaaa
     1021 tcaggcgcaa ccattcctaa aagttggcct cccctccggc gccaccatcg tctccgccga
     1081 cgtcgcgggg gaaaaagtta aaccagttca aggctccgat ggtgcccgcg tcccgctcct
     1141 tcgtcccggc ttccgcccca acggctccta cgccgtttcc ttcgtcttct tgcactccgg
     1201 cgcacccttc gcaaaaaagg gcggttccga tctttcgctt cctaaaatgg atgtgccgat
     1261 cgatctactg cagtgggaag tttttctgcc gcagcaatat aaagtgaaag acttcggcgg
     1321 cgacgccctc gccaccagcc tcctaccgcc tacctcgtgg gacagcgccg aggaagagcg
     1381 aggcttcggc tggggagttg gccacggcta tgcgcccccc gttaacgagc gcgacttcaa
     1441 gatgccatcg ctcgcgcccg gccaaatcgg cggcatcgtg gtcgacccga ccggcgcggt
     1501 cattccaagc gccaaagtca ccgtggttca tcaagacacc ggcacgactc ttaccacaac
     1561 aacggacgac tccggtcgct gggtcgtctc aaacgttcct tccggccgcg tacacgttac
     1621 ggccgcttcc ccaggattca aggactaccg ccaggatgcc aactacgatg caagtagacc
     1681 cctgcctctc agcttcaata tgaatctcgg tgccgcgact gaaaccgtaa cagtgatggc
     1741 cggcgtctcg ggcctgcaaa ccgaaagcgc ggaaatctcg tcacgaatcg agaagcaagc
     1801 tcgcaagcgc gccctcgaac aagaacgtgc cgcctcttcc aacgtcacca accttcagca
     1861 acgcgtcgct ggggttcttc cggtcgccgt cgacgtcccg catgccggca actcgtaccg
     1921 cttcgtccgc ccgctggtgc tcgacgaaga aaccaaagtc accttcacct acaaaacaaa
     1981 ataatccgaa aatcacactc catcggcccg tgagggcgcg gcgcctcaaa cttgctcgtt
     2041 gaccttcaac accagcaacg tttggtcgtc gcgggcctcc acatcgccgc aatgcgctct
     2101 tagcgtttcc gccagcactt cgcgaatctc gcgcgcacca ttcgttcgcg ttgcccgcaa
     2161 ggccctctct acgccctcgt agccgaactc atcgtcggcc cggttgccag cctccagaat
     2221 tccatccgtc aaaaacagga agacgtcccc cggctccgcc gctattcgca tctcctcaaa
     2281 ctccactccg ccaaacaatc ccagcggcat tcctaccgct tccaggattt ccactttccc
     2341 gcgccgaaat agcaccggct tcggcaatcc cgaattcgcg atgcgaaaat tcagcgtcgc
     2401 gtcgtcccac accgtgtaac acagcgccac aaaccggtcc tccatcgccc gctccatcag
     2461 cgattgattc agcgccttca gcatttctcc gggcgacagt tcgtgttctg tcaacgaccg
     2521 gatcagcccc atcgttagtg ccgcgtacag cgccgccgca gctcccttgc cggccacgtc
     2581 gcccagcatc cccacatgca aaccttgttt gcggtagtac cccaggtgca ccatgtcgcc
     2641 ggcgatgatt cgtgccgcgc agctcatcgt actcaccgcg gcatttttca tcgccggacc
     2701 gccgcttggc agcaaccgct tctgcaactc ccgtgctatc cgcaaatcct gattcagttg
     2761 cgcttcttgc gagctgatgc ggtcgtacaa aatcgcattg tccagcgcta ccgccagttg
     2821 cgaggccaga atcgtgatcg cctgtatgtg cccttcgcga aaataatccg gcgtcgcatt
     2881 ctcaatgtcc agcacgccaa ccagtttgtc cttggcgatc aacggcacgc tcagctccga
     2941 ccgcgtctcg tcatgaaact tgatgtagcg cgaatcttct cgcacgtccg gcaccactac
     3001 ggggtgtcgg taccgcgccg catatcccac cagcccgcgg tccagcggga tgtctggctt
     3061 ttcgatgact tgtcctcccg aaagcgaaaa ccggtgcttc agcgttttgc cgctggcatc
     3121 cagcagcaaa atagaaaagg tctggtattc cagcaggcga ctcaacaact ctccaatccg
     3181 tttcaacagc tcgtcggtat tcagcagcgc gctcagctcg cggctgatct cattcaggat
     3241 ctccagtgtc cgcgcccgcg cttcggccga ttgccgcgcc tgctgcaact cctgttctcg
     3301 ttcctttcgt tgcgaaatgt cgcgcacgaa agcgccaaaa agttgcccgc cctttccgtc
     3361 tagcggcccg ttgatcgtca gctccaccgg aatcagccga ccgtcccgat gcctcgcgca
     3421 cagctccagc cggtggtcca tcaccgccgc ctgatcgcgc ggaaaaaatc cctccacgcc
     3481 gccgcaattc gcctgccggt cttgctcggg aaagatagtt tcaaaaagag tttggccgat
     3541 ggcttcgtcc gcgttccagc cgaacgtagc cgccgcctgc aggttccaga tgacgatgcg
     3601 tttttcgctg tcaatgccga caaacgcatc cggagcagta tcaaggatga gctgcgcccg
     3661 cgcctcgccg tccgcaagac gctgcgctgc ctcgggttcc gtccgtcgcg gaaccccaaa
     3721 gggtacagac ctgggaactt gcatgacctc agggtaagtg cattcggtcg tttccgtcta
     3781 ccgtcaatgc gccaacgctc gtggttgaat cgacaacgcc actcctaacc ccgccccagc
     3841 tctctcgtct atcgttgtga acaccgtcta aatcgtcatg aaaacaacct cggcttgcct
     3901 ggctctgctg ttctgcttct ctgcaacttg ccacgctcag tcgtcaacca ttcccactag
     3961 ccaagacgat tccatggtcc tcatccacgg cgcgacgttt cagatgggca tcgatgcctc
     4021 cgaaattccg cgctttcaga aaatcttcaa cattgaccac ccgcaactgt tcaacgatga
     4081 acttcccaaa cactccgtca cggtcgccga cttctacctc gacaaacatc tcatcaccaa
     4141 cgcccagttc aaaaactttc tcctggccag acccagctgg cggcccacgc agctcccacc
     4201 tgatctcgac aacggccact acgctcaacg ttgggccgat ccgcaccagc ccaacctaaa
     4261 gcccgatcac cccgccgtga acgtcagttg gtacgtcgcc acggcattct gcggttgggc
     4321 cggtaaacgt ctccccactg aagccgagtg ggaattcgcc gctcgtggcg gccaggctgg
     4381 tgttctcttc ccctggggcg acgctcctcc cgaccaatct cgcgccaact tcgccgataa
     4441 cgttggcacc acaactccgg tcgaagccta tcccccaaat gcgttcggcc tctacgacat
     4501 ggccggcaat gcctggcaat tcctctccga cgagtggacg ccctatccct cccactcagg
     4561 cgacaccgtc ccgctacacg tcattcaaaa agagcccaag gacctcgtgc tccatcgcga
     4621 tgatcgcagg catgtcatcc gcggtggcag ctacgccggt gcccccatca acctctgggt
     4681 cgaataccgc gacagccacc ccgccaacgg ctcccaaccc ttcgttggct tccgctgcgc
     4741 caagtccgct tccgccccag gcaaaaagca ggaagtagac aggcgcggcg cccccaagat
     4801 ctgaaacgtc ggaacaagaa tttgtgatcg acaaaacttg cggaggcggg aacagcgttc
     4861 ccgcctccgc tgagctccgg gttcagcacc ccgcttgtaa agcgccgagc tgcccacccg
     4921 ggaccgcgat tttcgttgca ttccacttcc tggcttgcgc gatagaaatc ac
//
LOCUS       DASTPM010000382         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1195824, whole genome shotgun sequence.
ACCESSION   DASTPM010000382 DASTPM010000000
VERSION     DASTPM010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195824"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1503)
                     /locus_tag="VFR42_06685"
     CDS             complement(202..1503)
                     /locus_tag="VFR42_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0368875.1"
                     /translation="MNWSWLTVRVAIAATLLLLLFLSQRFWYRSLWRWSSKWGSRVLR
                     VAARLVYLAGLLLIIVSLADSIRNGHGRLLPRGTFLNVLAGLWFFSALFAYLAVKAVH
                     ALDRIWRFVRPSLKTAAASDLDLPRDPSRRYFFKTATAAAGAAPFIGAMYGFAAERLN
                     YQVHRVEIPVSNLPAALDGLKITQLSDIHLSGYMPRAQVRRAIEMANELGGDLAVVTG
                     DFITGADDPLEDCVDEIRRLSSPLGVYGCNGNHEIYADAEDRAQWLFAQAGMKLLRQD
                     NAVVSYRGAQLNLIGVDYQRERTPGGRRVSLLSGAQHLVRKDMPNILLSHNPNSFNRA
                     AELGVELSLAGHTHGGQVQVEILDHRLSPARFITDYVAGLYMRPLFTPARTVASQKFL
                     HATTLQGPHAAIYVNRGLGTVGAPVRLGVPPEISLLILRSA"
     gene            1759..2331
                     /locus_tag="VFR42_06690"
     CDS             1759..2331
                     /locus_tag="VFR42_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0368876.1"
                     /translation="MKRVAYVAAVCLAAFLGAMPVAAQTDAKPKEPPKPALGPSAAVL
                     DQWNDVGRKLVAIAEDLPEDKYDYKPNPDSRTFVANLLHAAGSMYFFTDAVNGQKARY
                     GDDPKRDELKTKAQVVAFVKKCVQDGADLIKSKGDAGMATAVNAGGPRLYRVDELAYG
                     LVEHSGEHYGQLVVYYRANGLVPPESRPKK"
     gene            2421..2606
                     /locus_tag="VFR42_06695"
     CDS             2421..2606
                     /locus_tag="VFR42_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368877.1"
                     /translation="MSKFVRLILAGAVCWLLAGCVPQDSLFPLILKSDQVFEEGILGE
                     WMVQGGTQEIKFDEKSG"
     gene            2652..2834
                     /locus_tag="VFR42_06700"
     CDS             2652..2834
                     /locus_tag="VFR42_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368878.1"
                     /translation="MPDIEEDGATLASIGSLVRLGSYLFIDVTSPDLERRKNAIVPYP
                     AIESHVCGRVQLEKDK"
     gene            complement(3071..>4101)
                     /locus_tag="VFR42_06705"
     CDS             complement(3071..>4101)
                     /locus_tag="VFR42_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368879.1"
                     /translation="MHHNPAKNVLWVAKEPSEIASQLGIEESVVRLTIARARQKMLAA
                     RLPRPTPYIDKTMYASWHAMFVSAYFEAARVFPNALGLSCRAFALKTLDRMLNEAWSE
                     GRGFLHRIGEGAELEGSLDDQVFGVIALLDAYEATLEERYFSAARRTMDIAIEKYGDS
                     ERGGFFDRSSDAPPMGGLDVRRKPFQDSPTPGGNSVAAIALTRLHAFTNDAKYAEWAQ
                     KTLEAFAGIAPQYGMFAATYGLAATLYAHHPAQVVITGAPNDPTAAALEAAANSVFRY
                     GKSVLRILPGVTPASLPAALRETLPHLPKDKTLALVCSGNTCFPPTGDADQLKELLTG
                     GRSSAAAR"
BASE COUNT          862 a         1117 c         1299 g          823 t
ORIGIN      
        1 ccacggcgtg cgatcggcgt cggcgagcgg aattccgtgc ttcatttttt cgacgttgac
       61 cggcggatga aaatcgtcgg cgtcgagaaa aatccagccg aggcggtcag cgatgagttt
      121 gccgaccgtg gttttgccag ctccggtggt gcccattacg aggatgacca tggtgatcgc
      181 gtttcgcgcg gcgtgcctgc gtcaggcgct gcgcaagatc agcagagaaa tttccggcgg
      241 gacgccaagc cgtaccggcg cgcctactgt gccaaggccg cggttcacat aaattgccgc
      301 gtgcggaccc tgcaaggttg tggcgtgcaa gaacttttgc gaagcgacgg ttcttgccgg
      361 agtgaacagc ggccgcatgt acaagcctgc gacgtagtcg gtgatgaagc gcgccgggct
      421 gagtcggtga tcgaggattt cgacctgcac ctggccgccg tgcgtgtgtc cggcgagaga
      481 aagctcgacg ccgagctcgg cggcgcgatt aaaggaattc ggattgtgcg aaagcaaaat
      541 gttcggcatg tccttgcgca ccaggtgttg cgcgccggag agcagactga cgcggcggcc
      601 gccgggagtg cgttcgcgct gatagtcgac accgatcaga ttcagctgcg caccacggta
      661 gctgacgacc gcgttgtcct ggcgcagcag tttcattccg gcttgtgcaa agagccattg
      721 cgcgcggtct tcggcgtcgg cataaatttc gtgattgccg ttgcaaccat agacgcctag
      781 cggcgacgaa agccggcgaa tttcgtcgac gcaatcttcc agaggatcgt ccgcgccggt
      841 gatgaagtcg ccggtaacta cagcgaggtc gccgcccagt tcattggcca tttcgatggc
      901 gcggcgaact tgtgcgcgcg gcatgtagcc gctgaggtga atgtcgctca gttgagtgat
      961 tttcaatccg tcgagcgccg cgggcagatt ggatacagga atttcgacgc ggtgaacctg
     1021 atagttcaaa cgttcggcgg caaatccgta catggcgccg atgaagggag ctgcgccggc
     1081 ggcggcggta gcagtcttga agaaatagcg gcggctggga tcgcgaggca aatccaaatc
     1141 cgacgcagcg gccgttttta gagagggccg cacaaagcgc cagatccggt cgagtgcatg
     1201 aacggctttt acggccaggt aggcgaagag cgcgctgaag aaccataggc cggcgaggac
     1261 gttcagaaaa gtgccacgcg gaagcaagcg gccgtggcca ttgcggatgg aatccgccag
     1321 gctgacgatg atcaggagca gtcccgcgag atagaccaat cgcgctgcga cgcgcaaaac
     1381 gcggctgccc cacttggagc tccagcgcca cagcgagcgg taccaaaatc tttgcgaaag
     1441 aaacaggagc agcagcaagg tcgccgcaat agcgacccgc accgtgagcc aggaccagtt
     1501 caaacttgcc tccagagcta ctcctttcag ggtaccatgc gcgtggcccg ctaaactttg
     1561 caggtgcagg cggtgccacc tgtcagtaag atggctggcg cggtggcggg cgccaagttg
     1621 taaagagttg tgaacgattg tgaaaggttg taaaaacgcg gccgccgggg cttacatcca
     1681 ttgcggccat ccgtcgaagc ggtcggtggc ctgtattctt cacagggagt tccctgccaa
     1741 aagggagagg gggagtgtat gaagcgtgtt gcgtatgttg ctgcagtttg cctggccgca
     1801 tttttggggg cgatgccggt ggccgctcaa accgacgcca agcccaagga gccgcccaaa
     1861 ccggcgctgg gtccttcggc ggcagtgctg gatcagtgga acgacgttgg acgaaagctg
     1921 gtcgccatcg cggaagactt gccggaagac aagtacgact acaaaccgaa tcctgattcg
     1981 cggacgttcg tggcgaattt gctgcacgcg gcggggtcga tgtatttctt cactgatgcc
     2041 gtcaatgggc aaaaggcccg ctatggggac gacccgaaac gcgatgagct gaaaaccaag
     2101 gcgcaagtcg tcgcgtttgt gaagaagtgt gtgcaggatg gcgccgacct gatcaagagc
     2161 aaaggcgatg caggcatggc cactgcggtt aacgcgggcg gaccacggct ctatcgggtc
     2221 gacgaattgg cttacggact ggtcgaacat tccggagaac actacgggca gctggtcgtt
     2281 tactatcgcg cgaacggcct ggtgccgccg gagtcgcggc cgaagaagta acaccttcac
     2341 atccgctgct ggacagtcta gagccgggga ggatcatctc taacgattcc cctcggcttt
     2401 tcctttctgg agacatgcgg atgagcaaat tcgtgcggtt gattctcgcg ggtgcggttt
     2461 gctggttgct ggcgggttgc gtaccacagg attcgttgtt cccgcttatc ttgaagagcg
     2521 atcaggtttt cgaggaaggt attttaggcg aatggatggt gcaaggcgga acgcaggaaa
     2581 taaagtttga cgagaaatcg ggttaggtga cttccgagag aggcgcgcaa gaccgtacgt
     2641 atgacgtcac catgcctgat atcgaagaag atggcgcgac acttgcgtcg attggcagcc
     2701 tcgttcgctt gggaagttac ctgttcatcg acgtgaccag ccccgatctg gagcgcagga
     2761 aaaacgcgat tgtcccgtat ccagcgatag agagtcatgt ctgcggccgc gttcaattgg
     2821 aaaaagacaa atgaggatcg attttctgaa tggcaactgg gtttcggacc aagcgaaagc
     2881 gggcaagctc gcccttgtca cagtgaaaac gccaaatttg gtgtcctagc agcttctact
     2941 gaagagctgc gaaaattcgc tctggaacac gcacacgaca aagacgcttt ttcagagaac
     3001 tttgctttcc aacggaagaa gtgaatgcct gacggtcggg tttatcctga ccgggacaga
     3061 acgcgagtcg ttaacgtgct gctgctgagc ttcgcccgcc tgtcagcaac tccttaagtt
     3121 gatccgcatc gccagtcggc gggaagcagg tattgccgga gcagaccagc gccagcgttt
     3181 tatccttcgg cagatgcggt aacgtttcac gcagggctgc cggcagcgag gccggagtta
     3241 cgccaggtaa tattcgcaag acggactttc cataacgaaa aactgagttg gccgcggctt
     3301 caagggcggc ggccgtcgga tcgtttggcg cgcctgtgat gacgacctgc gccggatgat
     3361 gcgcatacag cgttgcggcc aggccgtaag ttgcggcgaa cattccgtat tgcggcgcaa
     3421 ttcccgcaaa agcttccagc gttttctgcg cccattccgc atatttcgcg tcgttggtga
     3481 atgcgtggag ccgcgtcagc gctattgccg cgacggaatt gccacccggt gttggcgaat
     3541 cctggaaggg cttgcgccgc acgtcgagtc cgcccattgg aggcgcatcg ctggagcggt
     3601 cgaagaatcc gccgcgttcg ctgtcgccgt acttttctat ggcgatgtcc atcgtgcggc
     3661 gcgcggcgct aaaataccgc tcttccagcg tagcttcgta agcgtctaac agcgcaatca
     3721 cgccaaacac ctggtcatcg agtgagccct ccagctcggc gccttccccg atgcggtgca
     3781 ggaatcctcg tccttcgctc cacgcttcgt tcaacatgcg atccagcgtt ttcaaggcga
     3841 aagcgcggca actcaagccg agcgcattcg ggaacacgcg ggccgcttcg aaatacgccg
     3901 aaacgaacat ggcgtgccag cttgcgtaca tggtcttgtc gatgtaggga gtcggccgcg
     3961 gcaaacgcgc cgccagcatt ttctgtcttg cccgggcgat cgtcagacgc accacggact
     4021 cttcgatgcc cagttgcgag gcgatctcac tcggctcctt tgctacccac aaaacgtttt
     4081 tcgcgggatt gtggtgcatc t
//
LOCUS       DASTPM010000383         7343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_503735, whole genome shotgun sequence.
ACCESSION   DASTPM010000383 DASTPM010000000
VERSION     DASTPM010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503735"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VFR42_06710"
     CDS             <1..615
                     /locus_tag="VFR42_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0368880.1"
                     /translation="STIRRAVAELKKGRKLPFEEVQRICETEWSAIGYEDDYQEEEYK
                     KDGLEQLKTFYETLCAELPEILEQEKTFELDLENNVIVKGRIDQINSLGRKDVEIVDY
                     KTGKAKKDYEANKDLQLSIYAIAAKEIFELNPVRLVFHYLQDNKRQSTTRSAKQLTEA
                     QKIVQEVAAEIRAGEFAARPGFQCRSCAFRPICPAHEEALAERG"
     gene            complement(920..2071)
                     /locus_tag="VFR42_06715"
     CDS             complement(920..2071)
                     /locus_tag="VFR42_06715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0368881.1"
                     /translation="MKSPRKTEFPRSLPALIASTFLLFGVAHILAQDPPPAPVPKVNP
                     PPISQTPASPQQQTPPANPAPSDRQNTLVVNVNLVILPVTVKDGAGHLVADLTRTDFR
                     ILDDNVEQRLEYFSAEAFPLSAIVLLDNDLKSKDAQQVESSLSAIVAGLSTNDEAFVC
                     RFDTNFHPGKGFTRDQDKLLTELHRTRIDESRTSAPAPAAGGVFTSGPSINGQSVTSP
                     GAPITPGSTIILGDRTTKALDDAVYEAAQLLKDRGRDRRKIIFLISDGDNGKTNVNTY
                     DNTVKELLRYGISVYSIGVGSGFYERKFSRLQSYAHDSGGDVYYAAKRNALEELYSRV
                     TEQARNQYTLGYNPRGNDRGKDYHTVEVRVRREGLDVRTREGYYSAAPR"
     gene            2301..3269
                     /locus_tag="VFR42_06720"
     CDS             2301..3269
                     /locus_tag="VFR42_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0368882.1"
                     /translation="MPSLRNLWKLTVATALGASLAIPAVPQQPAQEPSQGPALKRTVN
                     LVNLFATVRDKSKRIVGDLKQEDFKVFEDNEEQKIAFFSKEVTMPITLGLLIDTSGSE
                     QNRLGAEQEAASRFLEHVMKKGDMAMVISFDLDVDLLADFTDDRGQIERAINKARIGA
                     VSGGVVTPGTIPSNTGGTHFYDAVYLACNDKLATEAGRKALVIITDAQDEGSKLRLEE
                     AIESAQRTDTVVHVLLVHDPGYSWRPDVAKKLSDETGGRTIEVSSQKKMTEAFDQISE
                     ELRSQYTVGYYPANTSKDGRFRKVRVETPGKEYKILTRKGYYAPRN"
     gene            3406..5862
                     /locus_tag="VFR42_06725"
     CDS             3406..5862
                     /locus_tag="VFR42_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368883.1"
                     /translation="MFSASQATTIKKIVLASFLAAVFSAGAAKPVPAQAAAFDLIGPR
                     VEMKVSRDGKSLPISEVANFQPGDRIWLHPDFPESQSVHYLLIVAFLRGSTNPPPEKW
                     ITLAETWNKKVKQEGILVTVPQDAQQVLVFLAPETGGGFSTLSRAVRAVPGVFVRASQ
                     DLNQAGLYRSRLEKYLSVVKETSDANPRELHDVSVKMAKTLGIKIDQQCFDRPAEQQG
                     SCLTQNTDRLVLDDGHSQSVVSALTNGPSADLVGAISTTPVAGGGFYSAYVGAVVDLA
                     RLLGNIHTAEFQYIPALALPKGEQLNLHLNNPPSFRNPKSVIVVGLPAVEAAQLPPLR
                     NPGVDQVHCLQNPSLVLPVEGAPLVFSTDLGHDFVLSLPTKKGATVELPAKADAARGG
                     FVVDVKSLALADLETQMVATLKGKWGFDTFAGPTFHLRNAQPDGWSLAAADQGALIVG
                     RDDTLHLQSICAVCAEKISLTLPDRKELTPTWKLSGTNEIEVHVPLKDAGTGPVTLQV
                     KQFGQSQADELKLHAYSEAAKLDEFSISAGDKQGVLEGTRLDQVNGFELNGIHFSPAK
                     LTRVERKDVLYLAAPDAKATENFQTNESLVARVALKDGRVLDLQTKVQPPRPRVTLVS
                     KSIQVGPNAAGIRLGDQDLLPQDGKITFFVKSDMPEKFGRGDKIEVAAEDESFRSTLG
                     VGEGNLVMQDAATVMAVFEPLKGFGASAFGPLRFRAISEDGRRGDWIPLAKLVRLPSL
                     KEIRCPDSPDQQCRLSGSSLFLVESVASDQQFTRNVPVPAGFADSSLGVPRPNGTLLY
                     LKLRDDPGTAVMAVLPVLPE"
     gene            complement(6206..6349)
                     /locus_tag="VFR42_06730"
     CDS             complement(6206..6349)
                     /locus_tag="VFR42_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368884.1"
                     /translation="MNNKMVFYIFGLIGILSGNINRNFVAIGTHEVDIPGSQIEAERD
                     SGR"
     gene            complement(6437..6607)
                     /locus_tag="VFR42_06735"
     CDS             complement(6437..6607)
                     /locus_tag="VFR42_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368885.1"
                     /translation="MGLAEGRSTYVPQANRSVHVGVLVWIANVDRKITGVKFYVLISS
                     DAFDRNVAGGYA"
     gene            6772..>7343
                     /locus_tag="VFR42_06740"
     CDS             6772..>7343
                     /locus_tag="VFR42_06740"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0368886.1"
                     /translation="MDGQTIGHYRILRKLGGGGMGVVYEAEDTKLGRRVALKFLPEDK
                     HRDPQTLERFLREARSASAMNHPGICTIHAIEEDSGKAFLAMELLEGQSLDAVLVNAP
                     LAFGRTLEIGIQLADALDAAHKKGIVHRDIKPANIFVTDRGTAKILDFGLAKLVTRPP
                     GTTEGATIDPDETHLTSPGMAVGTIAYMSP"
BASE COUNT         1565 a         1909 c         2397 g         1472 t
ORIGIN      
        1 tcgacgattc gacgcgctgt tgcggaactg aaaaagggcc gcaagctgcc gttcgaggag
       61 gtgcagcgga tttgtgaaac agagtggtcg gcgatcggct acgaagacga ctatcaagaa
      121 gaagagtaca agaaagacgg actggagcag ctgaagacat tttacgaaac gttgtgcgcg
      181 gaattgccgg aaattctgga gcaggagaag acgtttgagc tggatctgga aaacaacgtc
      241 atcgtgaaag ggcggatcga ccagatcaat tcgctgggcc gcaaagacgt ggagattgtt
      301 gactacaaga cgggcaaggc taagaaagac tacgaagcca acaaggattt gcagctcagc
      361 atttatgcca ttgcggcaaa agaaatcttc gagctgaatc cggtgcggct ggtcttccac
      421 tacctgcagg ataacaagcg gcagtcaacg acgcgcagcg cgaagcaact caccgaagcg
      481 cagaaaattg ttcaggaagt tgcggcggag attcgcgcgg gagagtttgc ggccagaccg
      541 ggatttcagt gccggagctg tgcttttcgc ccaatttgtc cggcgcacga agaagccttg
      601 gcggaacgcg gataatcgaa gctgcaatga aaaaggcggc gaaagctgag ggcccacgcc
      661 gccctttatt ttcaaattca gtgctgcggg ttatttgccc ttcttggggg ctttcttgtc
      721 ttccttctta gccttcttct tcattcagtg gctcctttat cgctgcggtt cccacgctta
      781 gcgcgtggtt tcagtgcgcg ggctggcggc gccggccacg tgctcctcat gcacgatata
      841 agtggtgcgc gcaggaggag tcaagaaaaa tcatgtgcgc tcggtaaaaa aagtttgtcg
      901 cggcaagcga tagcgatggt cagcggggag cggcggagta gtagccttcg cgggtgcgga
      961 cgtcgaggcc ttcgcggcgg acgcggactt ccaccgtgtg gtagtccttg ccgcggtcgt
     1021 tgccgcgggg gttgtagccg agcgtgtact gattgcgggc ttgctcggta acgcgcgagt
     1081 agagttcttc gagtgcattg cgcttggccg cgtagtaaac gtcgccaccg gaatcgtggg
     1141 cgtaggactg caggcgcgag aacttgcgtt cgtagaaacc gctgccgacg ccgatgctgt
     1201 agacagaaat gccgtagcga agcagctcct tgaccgtgtt gtcgtaagtg ttgacgttgg
     1261 tctttccatt gtccccgtcg gagataagga aaatgatctt gcggcggtca cgtccccggt
     1321 ctttgaggag ctgcgcggct tcgtatacgg catcgtcgag ggctttggtg gttcggtcac
     1381 cgagaatgat ggtggaaccg ggcgtgattg gggcgcccgg actggtgacg gactgcccgt
     1441 tgatcgaggg gccgctggtg aaaacaccgc cggccgccgg cgctggcgcg gaagtgcggc
     1501 tttcgtcgat gcgcgtccgg tggagttcgg tgagcaattt gtcctgatcg cgggtgaacc
     1561 ctttgccggg atggaagttg gtgtcgaaac ggcagacgaa ggcttcatcg ttggtgctga
     1621 ggccagcgac gatcgccgag aggctggact cgacctgctg ggcgtccttg gacttgagat
     1681 cgttgtccaa gaggactatg gccgacaggg gaaaggcttc ggcggagaag tattctaggc
     1741 gctgttcgac gttgtcatcc aggatgcgga aatcggtgcg cgtaaggtcg gcaaccaggt
     1801 gacctgcacc gtccttcacg gtgacgggca gaatgacgag attgacgtta actaccaggg
     1861 tgttctgcct gtcggaaggc gccggattgg caggcggtgt ttgctgttgc ggcgacgctg
     1921 gcgtctgcga aatcgggggc ggattgactt tggggacggg cgcgggaggg ggatcctgcg
     1981 cgagtatgtg agcaactccg aagagcagga aggtggaagc gatcagcgcg ggcagagacc
     2041 tgggaaattc tgtttttcgt ggacttttca tgttcaccca gccaaacttt tgctcgttct
     2101 ttgttcgtcg gggagcgctt tggccgccgc gcggggctca cgcatcccta gcgatgttcc
     2161 ttcgcatcta tgtaactcag ggtttgtaaa acaagccctg aaccctattg atgcagaaac
     2221 gacccagccc ggtgcgagcc gttttccggg actctcctgc agcctatggc cggctggcag
     2281 cgatcacaag gaaagtatac atgccttctc tgaggaattt gtggaagctg acggtggcga
     2341 ccgcgctcgg cgctagcctg gcgattcccg cggtgccgca acagcctgcg caggaaccgt
     2401 cgcaagggcc ggcattgaag aggacggtta acctcgtcaa cctgtttgcc accgtgcgcg
     2461 acaaaagcaa acgcatcgtg ggcgatctga agcaggaaga tttcaaagtg ttcgaggaca
     2521 acgaggagca gaagattgcg ttcttctcga aggaagttac gatgccgatt acgctaggcc
     2581 tgctgatcga caccagcgga agcgagcaga accggttggg cgccgagcag gaagcggcgt
     2641 cgcgcttctt ggaacacgtc atgaaaaagg gcgacatggc gatggtgatc agcttcgacc
     2701 tggacgtgga tttgctggcg gattttaccg acgatcgcgg gcagattgaa cgcgccatca
     2761 ataaagcgcg cattggcgcg gtcagcggcg gcgtggtgac gccggggacc atcccgtcga
     2821 ataccggggg gacgcatttt tatgacgcgg tgtatctggc ttgtaacgac aagctggcga
     2881 cggaagcagg gcgcaaggcg ctggtcatca ttaccgacgc gcaggacgaa ggaagcaagc
     2941 tgcggctgga agaagcgatc gaatcggcac agcgcaccga taccgtcgtg catgtgctgt
     3001 tggtgcacga ccccggctat tcatggcggc cggacgtggc gaaaaaattg agcgacgaaa
     3061 ccggcgggcg cacgattgaa gtctcttcac agaaaaaaat gaccgaagcg ttcgaccaga
     3121 tttccgaaga gctgcgcagc cagtacaccg tgggctacta tccggcaaac acgtcgaaag
     3181 atggtaggtt tcgcaaggtg cgcgtggaga caccggggaa ggaatacaaa atcctgacgc
     3241 ggaagggcta ctacgctccc aggaattgag cttttcattt ttattcttcc gaaaattagc
     3301 ggcaaataat cttggcgtga aattgtgatt cgctataatc aagagtgtag tgcaatcttc
     3361 taagcaagaa aaaatcagac ccccaggccg gaaagagaag caggagtgtt ttcagcttcc
     3421 caagctacca ccatcaaaaa gatagtactt gccagttttc tggcggccgt tttcagcgca
     3481 ggcgcggcga agccagttcc cgcgcaagcc gccgcgttcg acttgatcgg gccccgcgtg
     3541 gagatgaaag tttcgcgcga cggcaagtcg ctgcccattt cggaggtggc gaatttccag
     3601 ccgggcgatc gaatctggct gcaccccgat ttccccgagt cgcaatcggt gcactacctg
     3661 ctgatcgtgg ctttcctgcg cggctcgacc aatccgccgc cggagaaatg gatcaccctg
     3721 gcggaaacct ggaacaaaaa ggtaaagcag gaaggaattc tggtgaccgt gccacaggac
     3781 gcgcagcaag tgctggtgtt ccttgcgccg gagacaggcg gtggattttc gacgctgagc
     3841 agggcggtgc gcgcggtccc tggcgtgttc gtgcgggcgt cgcaggactt gaaccaggcg
     3901 ggcctgtacc ggtcgcggct ggaaaaatat ttgagcgtgg tgaaggaaac ttcggacgct
     3961 aatccacggg aactgcacga tgtttcggtg aaaatggcca agacgcttgg gatcaagatc
     4021 gaccagcagt gttttgaccg gccagcggag cagcaaggga gctgcttgac gcaaaatact
     4081 gaccggctgg tgctggacga tggacatagc caatcggtcg tgtcggcgtt gacgaacggc
     4141 ccctccgcgg acctggttgg cgccatcagc acgacgccgg tggccggggg cggattttac
     4201 agcgcgtatg taggcgcggt ggtggacctg gcgcggttgc tcgggaacat tcacactgcg
     4261 gagtttcagt acattcccgc gctggccctg cctaagggtg aacagctgaa tctgcacttg
     4321 aacaacccgc cgtcgtttcg caatccaaaa tcggtaattg tggtcggact gcctgcagtg
     4381 gaggcggcgc aattgccgcc gttgcgaaat ccgggagtgg accaggtgca ctgtttgcag
     4441 aatccgtcgc tggtcctgcc ggtggaaggc gcgccgctgg tgttctccac cgacttggga
     4501 cacgactttg tgctgagcct gccgacgaag aaaggcgcaa cggtcgagtt gccggcgaag
     4561 gccgacgcgg ctcgaggcgg tttcgtggtg gacgtgaaaa gtttggccct agccgatctg
     4621 gaaacgcaga tggtggccac gctgaagggc aagtggggat tcgacacgtt cgccggacca
     4681 accttccact tgcgcaatgc gcagccggac ggatggtcgc tggccgcggc ggaccagggg
     4741 gcgctgattg tcggtcgcga cgatacactg cacttgcaat caatctgcgc ggtatgcgcg
     4801 gagaaaatct cgctgacact gccggaccgc aaagaactta caccgacctg gaagctgagc
     4861 ggaaccaatg agattgaagt gcacgtgccg ctgaaggacg cgggcactgg gccggtgacg
     4921 cttcaggtga agcaattcgg acagagccag gccgacgagt tgaagctgca tgcgtattcg
     4981 gaggcggcta agctcgatga gttcagcatc agcgcgggag acaagcaagg cgtgctggaa
     5041 ggcacgcggc tggatcaggt gaacgggttt gagctgaacg ggatacattt ttctcccgcg
     5101 aagctgacgc gagtggagag aaaagacgtt ttgtatttgg cggcgccgga tgccaaggcg
     5161 acggaaaact tccagaccaa cgagagtttg gtggcgcgcg tggcgctgaa agatgggcgg
     5221 gtgctggatt tgcagacgaa agtgcagccg ccgcggccac gggtaacgct ggtgagcaag
     5281 tccatccaag tggggccgaa cgccgcggga atccggctgg gcgatcagga tttgctgccg
     5341 caagacggca agattacgtt ttttgtgaag tcggatatgc cggagaaatt tgggcgcggc
     5401 gataaaattg aagtggcggc ggaagacgag tcgttccgtt cgacgctggg cgtgggcgaa
     5461 ggaaatctgg tgatgcagga cgcggcgacg gtgatggcgg tgttcgaacc gctgaaaggg
     5521 tttggagctt cggcgtttgg gccgctgcgt ttccgggcaa tttcggaaga tgggcggcgg
     5581 ggcgactgga ttccgttggc gaagctggtg cggctgccgt cgctgaaaga gatccggtgt
     5641 ccggatagtc cggatcagca gtgccggctg agcggaagca gtttgttcct ggtggagtcg
     5701 gtggcgtctg accagcagtt cacgcggaac gtgccggtgc cagcgggatt tgccgattcg
     5761 tcgctaggcg tgccccggcc gaatgggacg ctgctgtact tgaagctgcg cgatgatcca
     5821 ggcaccgctg tgatggcggt gctgccggtg ctgcccgaat agcgctgagc gtctacagca
     5881 tgccccttga tcggtgttcc gtgccccatc gctcgacttt ccagcgaacc atcctccgtc
     5941 tcgccgccgt cctcgcgcgc gtcgaataaa ggacagggcg ttagagtacc ctcaggcagc
     6001 gcgaacccca ggaacctttc cgcacaccgc ctcgtatcct cccgtaagaa agagaggtgt
     6061 gcccatgcag ttcccttttt ccttctattt gttcaccctg gttcttgtcg ctagcactgc
     6121 agcgtcctcg cgcgcagccg gggacggtca agctgactcc agaaccgtta ccaccaaaac
     6181 gggtgagtcc catttcgagg ccccgctacc ttccggaatc cctctccgcc tctatctgcg
     6241 atccggggat atccacctcg tgggtgccga tggcaacaaa atttcggttg atatttccgg
     6301 aaagaatgcc gataagtcca aagatgtaaa acaccatctt attgttcaag acggctccgc
     6361 agaattccgg ctcagcggcg gaccgcgaaa cgactttcag atcactgtcc acctgccgaa
     6421 aaattgtgac cttttcctac gcgtacccgc cggcgacgtt acggtcgaag gcatcgctgg
     6481 agataaggac atagaacttc acgccggtga tctttcgatc cacgttggca atccagacga
     6541 gtactcccac gtggacgctt cggttagcct gggggacgta agtgcttcgc ccttcggcga
     6601 gtcccatagc ggacttttcc gttctttccg gaaatccggc tcaggcagat acaaacttca
     6661 cgcccacgtc ggcgctggcg acctgaccct gctttgattg gaggagagct tgggcgaatt
     6721 gaccggtgag gagctagccg ctaccatgct gcttctaata ttttccaaac tatggacggg
     6781 caaacgattg ggcattaccg gattctccgc aagctgggcg gcggaggcat gggcgtggtg
     6841 tatgaggccg aggatacaaa acttggccgg cgcgtagcgc tgaagtttct tcccgaggac
     6901 aagcaccgcg atccacaaac gctggaacgg tttctgcgcg aagcgcgctc ggcgtctgcg
     6961 atgaaccatc cggggatttg cacgattcac gcgattgagg aagacagcgg caaggcattt
     7021 ctggcgatgg aactgcttga ggggcagtcg ctggacgcgg tactggtaaa tgcaccgcta
     7081 gcgttcgggc gaacgctgga gatcggcatc caactggcgg atgcgctgga cgcggcacat
     7141 aaaaaaggaa tcgtacaccg cgacatcaag cccgcgaata tttttgttac cgaccgcggc
     7201 acggcgaaga ttctggattt cggtctggcg aaactggtta cgcgaccgcc gggaaccacc
     7261 gaaggcgcga ccatcgatcc ggacgaaacg catttgacgt cgccagggat ggcggtggga
     7321 acgatcgcgt acatgtcgcc gga
//
LOCUS       DASTPM010000384         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_734405, whole genome shotgun sequence.
ACCESSION   DASTPM010000384 DASTPM010000000
VERSION     DASTPM010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734405"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..1718
                     /gene="asnS"
                     /locus_tag="VFR42_06745"
     CDS             363..1718
                     /gene="asnS"
                     /locus_tag="VFR42_06745"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU0368887.1"
                     /translation="MTTITIEQAGKYDGQEVTLRGWLYNMRESGKLLFPIFRDGTGIM
                     QGVVSLKENPAAFEALKGLTQESSVIVIGKIRAEQRAPGGYEIGVIAVQVLQKVSEET
                     PFPIQLKEHGVDFLLDHRHLWLRTPRQAAILRIRAEAVRAARNYMDAQGYTLTDAPMF
                     TPSACEGTSTLFEVNYIDDEKAYLTQSGQLYIEATAMALGKVYTFGPTFRAEKSKTRR
                     HLTEFWMLEPEAAYATLDDMMALGEGLVSAVVQSVLQNKSRELELLKRDTKMLENVKP
                     PFPRINYEDAVDLLNKHGNPARSGDDFGGDEETIISKEFDRPVMIHRYPASIKAFYMQ
                     PDVEDPSKALAFDMIAPEGYGEIIGGSQRIHNYDLLVKRLKEHNLPEEAFQWYLDLRR
                     YGSVPHAGFGLGLERTVAWICGTEHIREVIPFPRMIYRVYPEPSSAVRSGWPLNGRAS
                     G"
     gene            1768..2679
                     /gene="rocF"
                     /locus_tag="VFR42_06750"
     CDS             1768..2679
                     /gene="rocF"
                     /locus_tag="VFR42_06750"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEU0368888.1"
                     /translation="MPQKVRIIGVPMDLGQSRRGVDMGPSALRVAGLQARIKQLGHTV
                     EDIGNLEVRQPEEMHVGEKRAKYLQEIAETCGDISKHVQKSLEEGFLPLVLGGDHSIA
                     AGVAAGVSSHFRKDKKQIGYIWLDAHGDMNTPESSPSGNVHGMPLAAIMGYGADELVM
                     LGGFKPKAEPGNIVIVGARDLDAQERKIVKKSGIHVFTMRDIDERGMREVMSDALKYA
                     MDDTDGVAVSLDMDFVDPSDAPGVGTPVRGGVTYREAHLACEMIADSEAMASLEVVEI
                     NPVIDEHNRTALLGVELVLSALGQKIL"
BASE COUNT          623 a          815 c          782 g          524 t
ORIGIN      
        1 gaccagtcct ttcgtgccca ccaggtccag caagcggcgt atatcggctt ccttcgctgg
       61 atcaattttc gtagcagcag ccacgtttgt cttgttggtc tgggcgttga caccgatagg
      121 agccagggcc aggcacacag ccagaacgaa actagccttc atgatttcct ccgtctttcg
      181 tgcgttctta gatgctagcg gaagggcgcg caaccccggg gcgtcctttt cgatgcgcac
      241 gcgaaacaac aacctgaacg aagggccagg gccgttcgta caggcggcgg gggctgtagc
      301 ggaaagcgcg cagggcaaat ctgataaact gctggtttcg cggcaagacg acgaagcgaa
      361 ccatgaccac gatcaccatc gagcaggcag gaaaatacga cggccaggaa gttacgctgc
      421 ggggctggct ctacaacatg cgagagtccg ggaagctgct cttccccata ttccgtgatg
      481 gaaccggcat catgcagggt gtcgtttcac tgaaggaaaa tcccgccgca ttcgaagccc
      541 tgaagggcct cacacaggaa tccagcgtca tcgtcatcgg caagatccgg gccgagcagc
      601 gcgcgcccgg cggctatgaa atcggcgtca ttgcggtaca agttctgcaa aaagtctcgg
      661 aagaaacccc gttcccgatc cagctcaaag agcatggcgt ggattttctg ctcgatcacc
      721 ggcatctgtg gcttcgtact ccccgccagg cagccatcct acgcatccgc gcagaagcgg
      781 tgcgtgccgc gcgcaattac atggacgcgc aaggctacac gctcaccgat gcgccaatgt
      841 tcactccatc ggcttgcgaa ggaacgtcga cgctctttga agtgaattac attgatgacg
      901 aaaaagcgta cttaacgcag tccgggcaac tatatatcga agccaccgca atggccctgg
      961 gcaaggtcta cacctttggc cccacattcc gtgcggaaaa atccaagact aggcgccatt
     1021 taaccgaatt ctggatgctc gaacccgaag ccgcctacgc gacgctcgac gacatgatgg
     1081 cgcttggcga aggcctggta tccgccgtag tgcaatctgt cttgcaaaac aagtcacgcg
     1141 aactcgaact tcttaagcgc gacaccaaaa tgttagagaa cgtaaagccg ccctttccgc
     1201 gcatcaacta cgaggacgca gtcgatctcc tcaacaagca cggcaatccg gccagatccg
     1261 gagacgactt cggcggcgac gaggaaacca tcatttccaa ggaattcgat cgaccggtaa
     1321 tgattcatcg ctaccccgcg tcgatcaaag ccttctatat gcagcccgac gtcgaagacc
     1381 cgagcaaggc tctggccttt gacatgattg cacccgaggg ctacggcgaa atcattggcg
     1441 gcagccaacg tatccacaac tacgatttac tggttaagcg attgaaagaa cacaacttac
     1501 cggaagaggc cttccagtgg tacctcgacc tgcggcgcta cggcagcgtg ccgcacgccg
     1561 gtttcggcct gggtctggaa cgcacggttg cttggatttg cggcaccgaa cacatccgcg
     1621 aggtgattcc cttcccccgc atgatttacc gggtgtatcc tgaaccttcc tcggcagtca
     1681 ggagtggctg gccgctgaac ggccgggcgt cagggtaggc gcccgctcgc cgcgcagccc
     1741 ggctggtcgc cgcatctgga aagtgcaatg ccacaaaaag ttcgtatcat tggtgtgccc
     1801 atggacctgg gccagagccg ccgcggcgtg gacatgggcc cgtcggccct ccgcgtcgca
     1861 gggctgcaag cgcgcatcaa gcaactcggc cacacagtag aagacatcgg caacctggaa
     1921 gtgagacagc ccgaggaaat gcacgtcggc gaaaagcgcg ccaaatactt gcaagagatt
     1981 gccgaaacgt gcggtgacat ttccaaacac gtgcagaaat cgctggaaga aggttttttg
     2041 ccgctcgttc tcggcggtga tcattcgatc gcggcgggag tggccgccgg tgtttcttcc
     2101 catttccgca aggataagaa acaaatcggc tacatctggc tggacgcaca cggcgacatg
     2161 aacacgccgg aaagctcgcc ctcgggcaat gtacacggca tgccgctggc cgccatcatg
     2221 ggctacggtg cggatgagtt ggtgatgctg ggggggttca agcccaaggc cgagcctggc
     2281 aacattgtga ttgttggagc gcgcgatttg gacgcccagg aacgcaaaat cgtgaagaaa
     2341 tccggcatcc acgttttcac catgcgcgat attgacgagc gaggcatgcg cgaagtgatg
     2401 tctgacgccc tcaaatacgc catggacgac accgatggcg ttgcagtctc tctggatatg
     2461 gactttgtgg atccatccga cgccccgggt gtcggtactc ccgttcgtgg cggcgttacc
     2521 taccgcgaag ctcacctggc ttgcgagatg atcgccgact cggaagccat ggcttccctg
     2581 gaagtcgtgg agatcaaccc ggtcatcgac gagcacaatc gcaccgcgct gctcggcgtg
     2641 gaactcgttc tttccgccct tggccagaaa atcctgtagg cgattccggc tcttcgctgg
     2701 atgccgccaa cccagcctgt cgccccgaca aatataaaga ttgc
//
LOCUS       DASTPM010000385         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_71013, whole genome shotgun sequence.
ACCESSION   DASTPM010000385 DASTPM010000000
VERSION     DASTPM010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71013"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFR42_06755"
     CDS             complement(<1..221)
                     /locus_tag="VFR42_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368889.1"
                     /translation="MFAIKRKFVTRLKIIPIALGAPIAPSHITPLLASTGLGLRSFPY
                     LYVLQAHAQKTTSAIGAEERAGNESLEGAV"
     gene            complement(515..2722)
                     /gene="ppk1"
                     /locus_tag="VFR42_06760"
     CDS             complement(515..2722)
                     /gene="ppk1"
                     /locus_tag="VFR42_06760"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU0368890.1"
                     /translation="MARTKLDDPRYYLNRHVQWLDFNRRVLEEARDPGNPLLERVKFL
                     AITANNLDEFVEVRVSSFLQRIEHGSTERSPDGLSAEEELALVTQSMHSLVRDQYKTW
                     NDELLPLLAKENIRVLRFSELDAKGVEFARKFYNRRVEPMLTPVTIDPSHPFPHVLNK
                     ALCISFLLQRKRGGNGKTYFGVLTVPRALPRLLRVPAAGNAIHYVPLQEIISYYAPKL
                     YRGYEILASAPFRITRNSNLYLEEEEARNLLEAIDSQVAQRRKGWAVRLEIEEGAHPD
                     IIKRLVSNFELDEPLVFRVRGLVNLQRLFHLYEETARPDLKFSPFAGKQVHIGHDADS
                     LFAALRHGDVLLHHPYESYDAVVNFLQTAARDPRVLSIKQTLYRTNTDSPIARALLEA
                     ASKKEVTVVVELKASFDEASNIRWARSFEDAGVQVFHGLVGLKTHAKLALVVREDPDG
                     KIRRYAHLGTGNYNPSTARFYSDFSLFTYDEAITASVNDVFNFLTAYAEQPDYKPLFV
                     APRDLAKNCIALIDRETHNAKRGRPARIVAKVNALVDPPMIQALYRASQAGVEIDLIV
                     RGQCALVPGLRGISSHIRVRSIVGRFLEHSRIFYFENGGESEIFLGSADWMQRNLYER
                     VEVVFPLKDPSLKQRIINEVLAAYLEDTRTARVLGPDGNFTRPRRVRNGHGVSVQEKL
                     MRAVQLPALAASSRGSKSANGKVPGILASAPSTKPESDEGADSMSHDTFSAAV"
BASE COUNT          608 a          758 c          837 g          603 t
ORIGIN      
        1 actgcgcctt cgaggctttc attacctgct ctttcctcag ctccgatagc cgaagtcgtt
       61 ttttgggcgt gcgcctgcaa aacatagaga tacgggaacg acctgagccc gagacctgtg
      121 ctggctaata gcggggtgat atgggaagga gcaattggtg cgccaagcgc aatgggaatt
      181 atcttcaacc gcgtcacaaa cttccgtttt atggcgaaca agcggaagcg tatttctttc
      241 ttttccccgg cgctttctgc ttaaggcgaa atccccacgc gaagcaagaa acctacacga
      301 aaatccccag ccaacggaag aatgccgcgt agcgcggctg acggaaaatt gtagtcaggc
      361 acagggcgcc ggaagtgcaa attcacgatg cgcggctggc cgcagtcatg catgtggaag
      421 gcctgcgtca tattctcacg ttcaacgcaa ccgatccgga gcccgtttga ttgcgtttca
      481 ctgagtccgt ctgaccctac ttgcgctggg tttgttacac tgcggcgctg aaagtgtcgt
      541 gcgacatgga gtcagcgcct tcgtcggatt cgggcttagt ggacggtgcg gaggctagaa
      601 ttccgggtac tttgccattc gcagatttgc tccctctgct gctggctgcg agtgctggca
      661 gttgtacggc acgcatcagc ttttcctgca cggaaacacc atggccgttt cggacgcgcc
      721 gtggtcgagt gaaattgcca tcgggcccga gcactcgggc ggtgcgcgta tcttcaagat
      781 aagcggccaa cacttcgttt atgatgcgtt gcttcagcga gggatctttc aagggaaata
      841 caacttccac gcgctcgtaa agattgcgct gcatccagtc cgcgctgccc agaaagattt
      901 ccgactcgcc tccattttca aaataaaaaa tacggctatg ctcgaggaac ctgccgacaa
      961 tactgcgcac gcggatgtga ctactgatcc cacgcaggcc gggcaccaag gcgcattgtc
     1021 cgcgcacgat cagatcaatt tcgacgcccg cctgcgaggc gcggtaaaga gcctggatca
     1081 tgggcgggtc gaccaacgcg ttgactttgg cgacgatgcg cgcgggcctg ccgcgcttgg
     1141 cgttgtgcgt ttcacggtcg atcaaggcaa tgcaattctt ggcgagatcg cgcggtgcca
     1201 caaacagtgg cttgtaatcc ggctgttcgg cataggcggt gagaaaattg aagacgtcgt
     1261 taacggaagc ggtgatagcc tcatcgtaag tgaacaaact gaagtcactg tagaaacgcg
     1321 ccgtggaagg attgtagttg ccggtgccca gatgagcgta acgacgaatt ttcccgtcgg
     1381 ggtcttcgcg tactaccagg gccagcttgg cgtgggtttt caacccgacc agtccgtgaa
     1441 acacctgcac gccggcatct tcgaagctgc gcgcccagcg gatgttcgaa gcttcgtcga
     1501 agctggcttt cagctccacc accacagtca cttctttttt cgatgccgct tcgagcaggg
     1561 cgcgagcgat gggcgaatcg gtgttggtgc gatagagcgt ctgcttgatg gaaagcacgc
     1621 gcggatcgcg ggcggcggtc tgcagaaaat tgacgaccgc gtcgtacgac tcgtacggat
     1681 ggtggagcag cacgtcgccg tgacgaagcg ccgcgaagag cgagtcagca tcgtgcccga
     1741 tgtgcacttg ttttccggcg aacggcgaga atttcagatc aggacgcgcg gtttcttcat
     1801 agagatggaa aaggcgctgg agattgacca agccgcgcac gcggaagacc aagggctcat
     1861 cgagttcaaa gttgcttacg agccgcttga tgatatcggg atgcgcacct tcttcgattt
     1921 ccaggcgcac cgcccagccc ttgcggcgtt gcgctacctg cgagtcaatc gcttcgagaa
     1981 gattgcgcgc ttcctcttct tccaggtaga gattgctgtt gcgcgtgata cgaaacggag
     2041 cggaagccaa aatttcgtag ccgcgataca gcttcggcgc gtagtaggag atgatttcct
     2101 gcagcggaac gtagtgaatg gcattacctg cagcgggtac ccgcagcagg cgcggcaatg
     2161 cgcgcggcac ggttaatacc ccgaaataag ttttgccgtt gccgccacgc ttgcgctgca
     2221 gcaggaaaga aatgcacagc gccttgttca gcacgtgcgg aaaagggtgc gacgggtcga
     2281 ttgttaccgg cgtgagcatg ggctcaacgc ggcgattgta aaacttcctg gcgaactcca
     2341 cgcctttggc atccagctca gaaaagcgca gcacgcgaat attctctttc gccagcaatg
     2401 gcagcagttc gtcattccag gttttgtact ggtcgcgcac cagtgaatgc atggactgcg
     2461 tcaccagggc cagctcttct tcggcggaga ggccgtccgg gctgcgctcg gtcgagccat
     2521 gttcaatgcg ctggaggaag ctggagacgc gcacctcgac gaattcgtca agattgttcg
     2581 cggtgatagc caaaaatttg acacgctcga gcagaggatt gccggggtcg cgcgcttctt
     2641 ccaacacgcg gcggttgaaa tcgagccact gtacgtggcg gttcaggtag tagcgcggat
     2701 catccaattt tgtacgtgcc atgagcaagc ctcggtgcga tggaacaaac cgtgggaacg
     2761 aacctatact ctctcgaaaa gcttgttaca gcgtgatgaa agacgg
//
LOCUS       DASTPM010000386         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1311019, whole genome shotgun sequence.
ACCESSION   DASTPM010000386 DASTPM010000000
VERSION     DASTPM010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1311019"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1429
                     /locus_tag="VFR42_06765"
     CDS             <1..1429
                     /locus_tag="VFR42_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0368891.1"
                     /translation="YGLCRVTGEPQLTRRHHRMSRISLILSRASMDSSFSPRDFLFTL
                     LLKVGVAASIAALLARWNTLRRVLFTEERDFDQKIKLMFFVTPPLILGVTLRLVGAPY
                     RFADLSLEGAFLMGLLGGRVVGPIGGAIITIPALIAHEWLAMPAASAAGLLGGLMRQA
                     IPNKEELWNFGPFTFLNIPKATLRFFRKAEISWEMLPLAACVGIELGRVGLVLATKPK
                     WLFAIDPQWNWWLCLAVIATLMSVAAPLKIWNNTRIEMNLERHQQLLLKARMDALSSQ
                     INPHFLFNTLNTVSALIRFDPDTARGVVLKLSNILRRILRKHETFVPLKEELQFIDDY
                     LDIEVARFGKDNLEIIKEIDNETLEAFIPSMLLQPMVENCLKHGLAPKIEGGRIRLHA
                     EQKDGRLKIEIEDNGVGISEERMPHVYVEGIGLSNVRERLRVLYGTDFRLEIESCPGQ
                     GTLIRIEVPELASTASPLEETVRTL"
     gene            complement(1437..3191)
                     /locus_tag="VFR42_06770"
     CDS             complement(1437..3191)
                     /locus_tag="VFR42_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368892.1"
                     /translation="MTVDWRWKRAARRRWSRFLRTPFGRLTQLFAGRVFHGGGESDSG
                     DLDLGVGVMVSLMAAPGLLVSLLTFEKYGSLIRFLNGSGVFDAFTATIADEYFFIVLS
                     LAVSGASALWRWDAIFLDRRDYTNLVPLPVRLRTIFLANFCAILFLTGLFTFVANIAS
                     AVLFPLAVVGSQNSASLFFHFAAGHLAGVVAGSAFGCLAVFALTGLLMALLPAALFRK
                     ISHLARFCAAIVLLALVASSFAVPELLARMPVADAHRIALLPPFSFLGLTRTVWGRGS
                     EPFVPEMFRGSLIALAGAFGTALLAYAASFRRSFLRIPELPDAGPLPRTGASFSPLAY
                     VHRMVLREPAQGACYDFVVRTLLRSESHLQILAGFAALGLVLAANALSSAPSLRALIT
                     GKVPSVDILSVPFILSFCMVAGVRFAFEIPADLRANWIFRFWLDREGHAARTVARYVL
                     LVFSIGWLAPLTFLATLCFWGWTTATMHTLILALSTSVVVELSLLRFRKMPFTCSYPP
                     FRSHSGLVVVAYLFAFLFFTGYLDEIERWSLFDPWRTLYFVPLGFGTLAGAHYYRKQM
                     LAMDKQLLFDEGPASAFW"
     gene            complement(3188..>3637)
                     /locus_tag="VFR42_06775"
     CDS             complement(3188..>3637)
                     /locus_tag="VFR42_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0368893.1"
                     /translation="SRKKKAVASLLRLFSLWPHRHVTMGSYSKGMRQRILLIAALMDN
                     PDILILDEPLSGLDVTSTLIIRNLIQTLSGRGKSIFYCSHILEVVEKVCSHLIILRKG
                     QVIAHGTTGEVREEIGKSSLEGVFLQLVADGDVAKVAEEIADVVSSP"
BASE COUNT          880 a         1170 c          959 g          628 t
ORIGIN      
        1 gtacggactg tgccgcgtaa ccggcgagcc acagctgacc cgtcggcacc atcgcatgtc
       61 gcgaatctct cttatactct ctagagccag catggactcg tctttctcac cgcgcgactt
      121 tctcttcacc ctcctgctca aggtcggtgt cgctgcttcc atcgccgccc tcctcgctcg
      181 ctggaacacg cttcgccgcg tgctcttcac cgaagagcgc gacttcgacc agaaaatcaa
      241 actcatgttc ttcgttacac caccgctcat cctcggtgtc acgctgcgtc tggtcggcgc
      301 gccgtatcgc ttcgccgatc tctccctcga aggcgctttc ctcatgggac tgctgggcgg
      361 ccgcgtggtc ggccccattg gcggcgccat catcaccatc cccgcactga tcgcccacga
      421 atggctcgcc atgcccgccg cttccgctgc cggacttctc ggcggcctga tgcgccaggc
      481 catacccaat aaagaagaac tttggaactt cggcccattc acgtttctca atattcccaa
      541 agccacactg cgctttttcc gcaaagccga gatttcctgg gaaatgttgc cgctggctgc
      601 gtgcgtcggg attgaactag gccgcgtcgg cctggtcctc gccaccaagc ccaaatggct
      661 tttcgccatc gatcctcagt ggaactggtg gctctgcctc gccgttatcg ccacgctcat
      721 gtccgtcgcc gccccgctca aaatctggaa caacacgcgc atcgaaatga acctcgagcg
      781 gcaccagcaa ctcctactta aagcccgcat ggacgcgctt tccagccaga tcaatccgca
      841 ttttctcttc aacacgctca acacggtctc ggcgctgatc cgcttcgatc cggataccgc
      901 tcgcggcgtc gtcttgaagc tcagcaatat cctccgccgc atcctccgca aacacgaaac
      961 gttcgttccg ctgaaagaag aattgcagtt catcgacgac tacctggaca tcgaagtcgc
     1021 ccgcttcggc aaagacaatc tggaaatcat caaggaaatc gacaacgaaa cgctggaagc
     1081 ctttatcccc agcatgttgt tgcagcccat ggtggaaaac tgcctgaagc acggcctggc
     1141 gcccaaaatc gaaggcggcc gcatccgcct ccacgccgaa caaaaagacg gccgcctgaa
     1201 gatcgaaatc gaagacaacg gcgtgggtat ttccgaagag cgcatgcctc atgtctacgt
     1261 ggaaggcatc ggtctaagta acgtccgcga acgcctccgc gtcctctacg gcaccgactt
     1321 tcgcctggaa atcgaaagct gcccggggca gggaactctc atccgcatcg aagtccccga
     1381 actcgcctcc accgcgtcgc ccttggaaga aaccgttcgc actttgtagg cgccgttcac
     1441 caaaacgccg aagcgggacc ttcgtcgaag agtagctgct tgtccatcgc aagcatctgc
     1501 ttgcgatagt agtgggctcc agccagtgtt ccaaagccca acggcacgaa gtagagggtt
     1561 cgccacgggt caaagagcga ccagcgctcg atctcgtcga gataacccgt gaagaaaagg
     1621 aaagcaaaca aataggcaac cactacaagg ccggaatgac tccggaatgg cggataggaa
     1681 caggtaaaag gcattttgcg gaagcgcagc agcgaaagct ccaccaccac actggtggaa
     1741 agtgccaaaa tcagtgtgtg catcgtcgcg gtggtccatc cccagaagca aagcgtagcc
     1801 agaaacgtaa gcggcgcgag ccacccgatg gagaaaacca gcaggacgta tctcgccacc
     1861 gttcgagcgg cgtgcccttc ccggtccaac cagaaacgga atatccaatt cgcccgcaaa
     1921 tcggctggaa tctcaaaagc aaagcgcacc ccggcgacca tgcaaaaact taaaatgaat
     1981 ggtacggaca aaatgtcgac agagggcact ttcccggtaa tgagcgcgcg caagctgggc
     2041 gccgacgaca gcgcgtttgc agccagcacc agtcctagcg ccgcgaatcc cgcgagaatc
     2101 tgcagatggc tctcgctccg cagcaatgtg cgcacaacaa agtcataaca ggcgccctgc
     2161 gccggctcgc ggagaaccat tctatggaca taggcaagcg gcgaaaacga agcgccagtt
     2221 cgcggaagcg gcccggcatc tggcagttcc ggaatgcgca ggaaggacct ccggaagctc
     2281 gccgcatacg ccagcagggc cgtgccgaag gcccctgcca gagcgatcaa gctgccgcga
     2341 aacatctcgg gcacgaaggg ctcggatcca cggccccaaa ccgttcgcgt caatcccaga
     2401 aacgaaaaag gcggcagcag cgcaattcgg tgggcgtcgg cgacgggcat ccgtgcaagc
     2461 aattcaggca cggcgaaact gctggcgacc agggccagca gcacaatcgc agcacagaaa
     2521 cgagccagat gagagatttt gcgaaagagg gccgccggca gcagcgccat caggagcccg
     2581 gtcaacgcga acaccgccag gcatccgaaa gcgctcccgg cgacgacacc ggccagatgc
     2641 cccgcggcga aatgaaaaaa caacgaagcg gaattctgtg agccgaccac cgcgagggga
     2701 aacagaaccg cggacgcaat gttcgcgaca aacgtaaaaa gcccggtcag aaacagaatc
     2761 gcgcaaaaat ttgccaggaa aatggtgcgc agtcttaccg gaagcggtac caggttagtg
     2821 tagtcgcgac gatcgaggaa aattgcatcc catcgccaca acgccgatgc tccgctcacc
     2881 gccagtgaaa gcacaatgaa aaaatattcg tccgcgatgg tcgcggtgaa ggcgtcgaac
     2941 actccgctgc cgttcagaaa acgaatcagc gacccgtatt tttcaaacgt caagagcgac
     3001 accagaagtc ccggcgccgc catcaagctc accatcacgc cgacgccaag atctaaatcg
     3061 cccgaatcgg attcacctcc gccgtggaac acccggccgg caaacagttg cgtcagccgc
     3121 ccgaacggcg tgcgcagaaa cctcgaccag cgccttcgtg ccgctcgctt ccatcgccag
     3181 tccaccgtca tggcgaactc accacatctg caatttcttc ggcgaccttc gccacatctc
     3241 cgtccgccac cagctgcagg aacacgcctt ccagagaaga tttcccgatc tcttcgcgca
     3301 cttcgcccgt ggttccgtgc gcaatcacct gtccttttcg cagaatgatc aggtgcgagc
     3361 aaaccttttc caccacttcg agaatgtggg aacagtaaaa aatggatttg ccgcggccgc
     3421 tcaaggtctg gataaggttc cgaattatca gagttgacgt gacgtccagc ccggaaagtg
     3481 gttcatccag aatgagaatg tccggattgt ccatcagcgc cgcgatcagc aggatgcgct
     3541 gccgcatgcc cttcgaatac gaacccatgg tcacgtgccg gtgcggccac agcgagaaca
     3601 gacgcaaaag ggaagcgacg gcttttttct tgcgtga
//
LOCUS       DASTPM010000387         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_474949, whole genome shotgun sequence.
ACCESSION   DASTPM010000387 DASTPM010000000
VERSION     DASTPM010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474949"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFR42_06780"
     CDS             <1..595
                     /locus_tag="VFR42_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU0368894.1"
                     /translation="FVRQMFQGKTAADVSFVGAGPWFASFQSGAFRADQVAAAGGGAR
                     PINKVSVDLRPEQIRTKPLELFKEAKSAVDLTQAPLIVAIGRGIKAPENIPQAEAVAK
                     AMGAEIAASRPICDEGWLPMERQIGSSGQTVAPKLYLALGISGAIQHVVGMKGARTIV
                     AINKDQNAPIFEIADYGVVGDIFEIMPVLAEELQKAK"
     gene            718..1095
                     /locus_tag="VFR42_06785"
     CDS             718..1095
                     /locus_tag="VFR42_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0368895.1"
                     /translation="MSDTPTYPYETRLNIFYQPLEIVEEKALADRCAYRWYNQTLCQV
                     NGSVVRLGVVQGEYHWHKHDDDDEFFYVVEGKLLIDLEGRTLQLGPRQGTVVPKGVLH
                     RTRAPERTVMLMVENAGIIPTGN"
     gene            1242..2918
                     /locus_tag="VFR42_06790"
     CDS             1242..2918
                     /locus_tag="VFR42_06790"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEU0368896.1"
                     /translation="MTTQREQLEADVLIVGAGPAGLSCALHLANLIKKHNDSGATPQL
                     SAENIYVLEKGREIGAHQLSGAIMNPKALAELVPDFQKSAPLDTPVTDSAALFFTQNS
                     SFRFPITPPPFQNKGNYVVSLNKIVKWLGGLVESAGVNVFKEFGGAALVYGENGGVAG
                     VITEDKGLDKNGKPKDNYTPGYELRAKITVLAEGTRGSLTKKLVAEKKLDNLNPQSYG
                     IGIKELWDIQPGRVEPGYVAHTLGWPVSTDMYGGGWIYGLSNNRLSIGLVLALEYADP
                     QFDPHAAFQKWKTHPHLKKNLEGGRLVRYGAKSLPYGGWYAMPRNTVDGVLIIGDSGS
                     FLDSQRLKGIHLAIKSGMLAAETIFESLKTGDNTTKTLGGFQQRVEQSYIKDELWKVR
                     NFHQSFQHGTFRGFVHTAFQQITGGRGLIDPMRAEAGYKHYKKLASPAAAPANPEDRF
                     KGDGKLTFDRLTDVYHSGTRHNDDQPCHLHILDSNICITKCVEEYGNPCQYFCPAAVY
                     EMAKESIGALKLKINFPNCVHCKTCDIADPYQIIDWVVPEGGGGPNYEGM"
     gene            complement(3024..>3902)
                     /locus_tag="VFR42_06795"
     CDS             complement(3024..>3902)
                     /locus_tag="VFR42_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368897.1"
                     /translation="AATLALSSKFNTSPNGAQSGIWMSGGAPAADSSNNLYVMTGNGS
                     FDADTGGSDYGSSYLKLSTASGLTVSDYFTPHDQLSLSMNDRDVGAGGTALLFGQNLM
                     VGAGKSGAFYVLNRSNMGHFNESSDAAAVQTWMAGHAFSTPAFWNNTMHYFGSSRPGV
                     AYDFNPSTGLFNIVPSSQTPSGYGFPGATPSVSSSGSTNGIVWAIDSNAFCTGQARAC
                     GPAVLHAYDANNLATELWNSTQGSGNAAGLAVKFTVPTVANGKVYVGSRGNNTGGDDS
                     STSIPGQIDVYGLLPN"
BASE COUNT          874 a         1270 c         1008 g          750 t
ORIGIN      
        1 tttcgtccgt cagatgttcc agggtaaaac tgccgccgat gtttccttcg tcggcgccgg
       61 cccgtggttc gcttccttcc aatccggcgc atttcgcgcc gaccaggtcg ccgcagccgg
      121 cggtggcgca agaccaatca acaaggtcag cgtcgatcta aggcccgaac aaattcgcac
      181 caaaccgctc gagcttttca aggaagcgaa gtccgccgtc gatctaacgc aggctccgct
      241 aatcgtagcg atcggtcgcg gcatcaaagc tcctgaaaat atcccccagg cggaagccgt
      301 cgccaaagct atgggcgcgg aaattgcggc gtcacgccct atttgcgacg aaggctggct
      361 ccccatggag cgccagatcg gcagctctgg ccaaaccgtc gctcccaagc tctatctcgc
      421 gctcggcatc agcggcgcca tccagcacgt cgtcggcatg aagggcgcgc gcaccatcgt
      481 cgccatcaac aaggatcaga atgcgccgat tttcgaaatt gccgactacg gtgtagtcgg
      541 agacatcttc gaaatcatgc ccgtgcttgc ggaagaactg caaaaagcca agtaaacgcc
      601 gccgttctcg cgctttttgc gtgcgctaag cagcgagaca ttttgactct tgcggtccac
      661 ctattcaggc tggaaagtag gatgtagaag ttgcctctgc agcacgccgg aggggccatg
      721 tccgacacgc cgacctatcc ctacgaaacg cgcctcaata ttttttacca gccgctcgaa
      781 atcgtcgaag aaaaagctct tgccgaccgt tgcgcctacc gttggtacaa tcagacgctg
      841 tgccaggtga acggctccgt cgtgcgcctc ggcgtagtgc aaggcgaata tcactggcac
      901 aaacacgacg atgacgacga attcttttat gtggtcgaag gtaaactcct gatcgatctc
      961 gaaggccgaa ccttgcagct cggtccacgc cagggcaccg tcgttcccaa aggcgttctc
     1021 caccgcactc gcgcccccga gcgaaccgtc atgctcatgg tcgaaaatgc cggcatcatt
     1081 cccaccggca attagggcca accgccgagc attcatggcg gacgggaaat cgggccgccc
     1141 ctgttcttca agatactaag tcaatttggc ctgaccccgg ccgagttcga aaagctccgc
     1201 tggcaaccag ccgcgtcaat cccttgctaa cataggattc gatgaccacc caacgcgaac
     1261 aacttgaagc cgacgtactc atcgtcggcg ccggccccgc cgggctaagc tgcgccctcc
     1321 atctcgccaa cctcatcaag aagcacaacg actccggcgc cacgccacaa ctttccgcgg
     1381 aaaatatcta cgtcctggaa aaaggccgcg aaatcggcgc gcaccagctc tccggcgcca
     1441 tcatgaaccc caaagccctc gccgagcttg tacctgattt tcagaaatcc gccccgctcg
     1501 acacccctgt caccgattca gccgcgcttt tcttcacaca aaattcctcg ttccgctttc
     1561 ccatcactcc gccgcctttt caaaacaaag gaaactacgt cgtctctctc aataaaatcg
     1621 tgaagtggct cggcggctta gttgaatccg ccggcgtcaa tgtcttcaag gagtttggcg
     1681 gcgccgcact ggtctacggc gaaaacggtg gagtagcggg cgtcatcacg gaggacaagg
     1741 gcctcgacaa aaacggcaag cccaaggaca actacacccc cggctacgaa ctgcgcgcga
     1801 agatcaccgt tctcgctgaa ggcacccgcg gctcgctcac caaaaagctc gtcgccgaaa
     1861 aaaaactcga caacctcaat ccgcaaagtt acggcatcgg catcaaggaa ctctgggata
     1921 ttcaacccgg acgcgtcgag cctggctacg tcgcccacac gctcggctgg cccgtttcaa
     1981 ccgacatgta cgggggcggc tggatctacg gcctctccaa caatcgtctc tccatcggcc
     2041 tggtcctcgc tctcgagtac gccgacccgc aattcgatcc gcatgctgct ttccagaagt
     2101 ggaagacaca cccgcatctc aaaaaaaatc tcgagggcgg aagactcgtc cgttacggcg
     2161 caaagtccct cccctacggc gggtggtacg ccatgccgcg caacaccgtc gacggcgtcc
     2221 tcatcatcgg cgattccggc agcttcctcg attcgcagcg cctcaagggc atccatctcg
     2281 cgataaagag cggcatgctc gcggccgaaa ccattttcga atcgctcaag acgggcgaca
     2341 acacgacgaa gactctgggt ggcttccagc aacgcgtcga acagagttac atcaaggacg
     2401 agctatggaa ggtccgcaac ttccaccaat cgtttcaaca cggaacgttc cgcggcttcg
     2461 tccatactgc ctttcagcaa attaccggcg gtcgtggcct catcgacccg atgcgcgccg
     2521 aggccggcta caagcattac aagaaacttg cgtctcccgc ggccgcgcca gccaatcccg
     2581 aagaccgctt caagggcgac ggcaagctca cgttcgaccg cctcaccgac gtctatcact
     2641 ccggcacgcg ccacaacgac gaccagccct gccacctcca cattctcgat tcaaacatct
     2701 gcattacgaa atgcgtggaa gaatacggca acccctgcca atacttctgc cccgccgccg
     2761 tttacgagat ggccaaagaa tccatcggcg ccctaaaact aaaaatcaat tttcccaatt
     2821 gcgtgcactg caagacctgc gacatcgccg acccctacca gatcattgac tgggtcgtac
     2881 ctgaaggcgg cggcggcccc aattacgaag gcatgtaacc gccggcggcg cgcattttct
     2941 cccgcgcgtt aaaattagac tcggcgcatc cactcgaaac aaaaagccct ggcttatcgc
     3001 cagggctcct cgctgcgaat ggctcaattc ggcagcagtc cgtagacgtc gatttggccc
     3061 ggtattgacg ttgaactatc gtcgccaccc gtattgtttc cacgactccc cacgtagacc
     3121 ttgccattgg ccaccgtggg aacggtaaat ttgaccgcaa ggcccgccgc attgccgctt
     3181 ccttgcgtgc tgttccaaag ttccgtcgcc agattgttcg catcataggc atgaagtacc
     3241 gccggtccgc acgcccgagc ctgccctgta caaaatgcgt tggagtcgat cgcccagacg
     3301 ataccgttcg tcgaacctga agaggatact gacggtgtcg cgcccgggaa accgtacccg
     3361 gagggggttt gcgaggaagg cacgatgttg aacaagccgg tcgatgggtt gaagtcgtag
     3421 gccaccccag gccgggagga gccgaagtag tgcatcgtgt tgttccaaaa cgcgggcgtg
     3481 gagaaggcgt ggcctgccat ccaggtttgt accgccgcag catcggaaga ctcgttgaaa
     3541 tgccccatat tgcttcggtt cagcacataa aaggcgccgc tctttccggc tcccaccatc
     3601 aaattctgtc cgaagagcaa agccgttcct ccggcaccta cgtcccggtc gttcatgctc
     3661 aaactaagtt gatcgtgcgg cgtaaagtaa tcgctcaccg tcagcccgct ggcggtggag
     3721 agcttgagat agctgctgcc gtaatcgctc ccgccggtat cggcatcgaa cgatccgttg
     3781 ccggtcatga cgtacaaatt gttgctgctg tctgcagccg gcgcccctcc ggacatccag
     3841 atgccggatt gcgcgccgtt cggagacgta ttgaacttac ttgagagtgc cagcgttgct
     3901 gc
//
LOCUS       DASTPM010000388         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_129233, whole genome shotgun sequence.
ACCESSION   DASTPM010000388 DASTPM010000000
VERSION     DASTPM010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129233"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFR42_06800"
     CDS             <1..606
                     /locus_tag="VFR42_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368898.1"
                     /translation="GGGWKHVYKVHDKQELIEAYDKTAPYCMTLQEFIDFDRYVRCFT
                     FGKSDIVPVHYDPKERRYLVDHDYLTPALDARVVKDAQTINQALGYEMNTIEFAVQGD
                     VPYAIDFLNPAPDFERDRITEFYFEMVVDRMARLVIDRALNGVASNPWPRWEEMLGLG
                     AASGVIGAPKGVAQVATASVTTSVPATTSGANSADAGAKIK"
     gene            603..2000
                     /locus_tag="VFR42_06805"
     CDS             603..2000
                     /locus_tag="VFR42_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368899.1"
                     /translation="MSSGAAPTNPPSTVSSPPAELWHKLLQRELQRSPKLTEEFYDKL
                     RAKKLTFGNRVHCPFLRPFFLSPEDEQRVRLVAEKMAELGERVATHAMQDAALLNQLH
                     LREQELRLIRLGKLHGFASTASRLDSFLLPDSLNFAEYNGESPAGSGYTETLAEVFRS
                     LPVMAEFAKSYEVHSYPLSAKLLDALIMAYLDWGGASKKPQMLITDWREVPTWNEFEI
                     LKARFEKMGVPVELADPRDLQFDGKALLANGKKIDLVYRRVLINDIAARPAECKPLVD
                     AVAKNSVCMANHFRCKIPHVKAFFAVLTDERNDALFSFDERGLIRNHVPWTRVVADTR
                     TSHHGVPIELLDFIRRQRQNLVLKPSDEYGGSGVTLGWGTSESEWDTVIEKAIAATQG
                     CWIVQERIPIRREVFPWVRPDGTVDYRDMLVDFAPYLFRGKVCGFLTRLSATGLANVT
                     SGGGQIPAFRVHPRT"
     gene            2094..>2729
                     /locus_tag="VFR42_06810"
     CDS             2094..>2729
                     /locus_tag="VFR42_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368900.1"
                     /translation="MPGAKNAVDTCLAVQPDEHVALIADKTSAAVAASLSEALSQIHA
                     RTTSLLLEDFGPRPMRGAPQPVLDELEKADVGILAMNPQPGELAARMSIVQVVERRRI
                     RYAHMVGVTPQIMQQGMRADYKLVDRLSDRLRERMLRADTLTVKTEAGTSFAAHFDRK
                     LDWVKTSGLISPRYWSNLPAGEVFTTPANVDGTFICDATAGDYFNGKYGDLQ"
BASE COUNT          592 a          880 c          740 g          517 t
ORIGIN      
        1 ggcggcggct ggaaacacgt ctacaaagtt cacgacaagc aggaactgat tgaagcctac
       61 gacaagaccg cgccgtactg catgacgctg caggaattca ttgatttcga ccgctacgtg
      121 cggtgcttca ccttcggcaa atcggacatc gtgccggtgc actatgatcc gaaagaacgc
      181 cgctatttgg tcgaccatga ctatctgaca ccggctctgg acgcccgcgt ggtgaaagac
      241 gcgcagacca tcaaccaggc gctaggctat gaaatgaaca ccatcgagtt tgccgtccag
      301 ggcgacgtgc cctacgccat cgattttctc aaccccgccc ccgacttcga gcgcgatcgc
      361 atcacggaat tttatttcga gatggtcgtc gacaggatgg cgcggctcgt aatcgaccgt
      421 gctttgaatg gcgttgcgtc caatccctgg ccccggtggg aagaaatgct gggcctagga
      481 gcggcatccg gggtaatcgg cgcgcctaag ggagtggcgc aggtagcaac cgccagcgta
      541 accacgtccg tgccggcgac gacttcaggc gccaactccg ctgacgccgg agccaaaata
      601 aaatgagcag tggcgccgct cccaccaatc cgccgagcac cgtctccagc ccgcctgcgg
      661 agctgtggca caaactcctg cagcgagagc ttcagcgttc gccaaaactc accgaagaat
      721 tttacgacaa gctgcgcgcc aagaagctga cctttggcaa ccgtgtgcac tgcccgtttc
      781 tccggccttt ttttctatcg cccgaagacg agcaacgcgt gcgcctcgtg gccgaaaaaa
      841 tggcggagct tggcgagcgc gtcgcgaccc atgcgatgca agatgcggca ttgctgaacc
      901 agttgcactt gcgcgagcaa gaactgcgcc tcatccgcct gggcaaattg cacggctttg
      961 ccagcacggc gtcgcgcctg gactcatttc tacttcctga ttcgttgaac tttgcggagt
     1021 acaatggcga gtcccccgcg ggctccggct acaccgaaac tctagcggaa gtctttcgca
     1081 gcctgccagt tatggcagag tttgccaaga gttatgaagt ccacagctat ccactcagcg
     1141 cgaagctgct ggacgcattg atcatggcct acctcgactg gggcggcgct tcgaaaaaac
     1201 cgcagatgtt gatcaccgac tggcgcgagg tccccacttg gaacgaattt gaaatcctga
     1261 aggcgcgctt tgaaaagatg ggtgtgcccg tggagctggc cgatccgcgc gatctgcagt
     1321 tcgacggcaa ggcgctgctc gccaacggca aaaaaattga cctggtttat cgccgcgtcc
     1381 tcatcaacga catcgcggct cgcccggcgg aatgcaaacc gctggttgac gcggtcgcta
     1441 aaaactccgt ctgcatggcc aatcatttcc gctgcaaaat cccgcacgtc aaagcgttct
     1501 tcgcggtgct caccgacgag cgtaatgacg ctcttttttc tttcgacgaa cgcggattaa
     1561 tccgcaatca tgtcccctgg acccgcgtcg tggccgatac ccgcacctcg caccacggcg
     1621 tgcccatcga actcctcgat ttcatccgcc gccaacgaca gaacttggtg ctgaagccca
     1681 gcgacgaata cggggggagc ggcgtcacgc tcggctgggg aaccagcgaa tccgaatggg
     1741 acactgtcat cgaaaaagct atagccgcca cgcaaggctg ctggatcgtg caggagcgca
     1801 tccccatccg ccgcgaagtt tttccgtggg tgcggcccga cggcacagtc gactatcgcg
     1861 acatgctcgt cgatttcgcc ccctatcttt tccgcggcaa agtctgcggc ttcctcaccc
     1921 gcctcagcgc caccggcctg gccaacgtca ccagcggtgg cggtcagatc ccggctttcc
     1981 gcgtccaccc gcgaacgtaa aaagcgaaac tatctgcgcc gaatcgctac aaaattccac
     2041 tatgaacctg taaaataatt aggatgtttt tcggatcgct ccagccagag ctcatgcccg
     2101 gcgcgaagaa cgccgtagac acttgtctcg ccgtccagcc ggacgaacac gtcgcactga
     2161 tcgccgataa aaccagcgcc gcggtagccg cgagcctctc cgaagccctt tcgcaaatcc
     2221 acgcgagaac aacttctctt ctgctcgaag atttcggacc gcgtcccatg cggggcgctc
     2281 cacagcccgt gctggacgag ctcgaaaaag ccgatgtcgg cattctcgcg atgaacccgc
     2341 agcccggcga actcgctgcc cgcatgtcta tcgttcaggt cgtcgagcgc cggcgcatcc
     2401 gctacgccca tatggtcggc gtcaccccac aaatcatgca gcaaggcatg cgcgccgatt
     2461 acaaattggt tgatcgcctg agcgaccgcc tgcgcgaacg catgctgcgc gccgacacgc
     2521 tcacggtaaa aaccgaagct ggaacttcct tcgccgcaca cttcgaccgc aaactcgact
     2581 gggttaagac cagcggcctc attagcccgc gctattggtc aaaccttccg gcgggcgaag
     2641 tcttcaccac tcctgcaaac gtcgacggca cattcatctg cgacgccact gctggcgact
     2701 acttcaacgg aaaatatggc gacctgcag
//
LOCUS       DASTPM010000389         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1368931, whole genome shotgun sequence.
ACCESSION   DASTPM010000389 DASTPM010000000
VERSION     DASTPM010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368931"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFR42_06815"
     CDS             complement(<1..510)
                     /locus_tag="VFR42_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU0368901.1"
                     /translation="MSKIIGIDLGTTNSVVSVMQGGEPVVIPNQEGARTTPSVVAITK
                     TGERLVGQVAKRQAVTNPENTVYSIKRFMGRRFDEVGSETKRVPYKVVKGDHDDARVE
                     ISGKIYSPPEISAMILTKLKNAAEDFLGEKVTKAVITVPAYFNDAQRQATKQAGEIAG
                     LEVVRIINEP"
     gene            905..1693
                     /locus_tag="VFR42_06820"
     CDS             905..1693
                     /locus_tag="VFR42_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU0368902.1"
                     /translation="MKGRHLAAIAIALVVVGSLLFFGVSGRQDPVANQAAAIPPKVEA
                     ASLPAVTPPAAAPSASELTSPPATAAALQPATPAQGSGVTDVDPRRAYGSKNAPISLE
                     VFSDFQCPACKQLYKTTNQLLLDNYVNTNKVYLIHRDFPLPMHAYSKIAARYVRAASQ
                     LGKGEQAEKALFDNQEKWEQSGDVDGTLAGALSAAEMTKIRAVAKGTSLDAAIEKDVA
                     LGKGYNVNQTPTSVIHFKGQTYPVVGVVSYDVLHSFLDQLLSQK"
     gene            1721..2242
                     /locus_tag="VFR42_06825"
     CDS             1721..2242
                     /locus_tag="VFR42_06825"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEU0368903.1"
                     /translation="MMQSSPLNFRRAVIWLGRLLIGGIFVYAGYAKLVYPNHNLWPWF
                     MLKFSVSANLSTFAFQVESYKILGAAASSFVAHTLPFVEIVLGLLLLVGWRLRIWATI
                     VSAILVGFLGAVTRAYLLHMDINCGCFGTPEPLTIMTVLRDSALLLLALVMTVFAYQE
                     ARTAHPWTAATNS"
     gene            2377..>3340
                     /locus_tag="VFR42_06830"
     CDS             2377..>3340
                     /locus_tag="VFR42_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU0368904.1"
                     /translation="MLSLPPITLRLRGWKLIDRVALAFLLLCSIALGAACGLLFVYAS
                     DLPEIRALETYRPNVVTEIYADDGQMVGSFALQRRILMTYEQCPKILFKAVTSIEDQH
                     FEDHWGIDFPRIAGAAYRNLLKGRKAEGASTITMQLAGNLFLDRSDRSFRRKMQEMLL
                     SLQIERRYTKPQIFAMYANQVYLAHGNYGFAAAAQFYFGKPVTDLKLQEAALLAGMVN
                     GPKFSPLLNPELALSRRNLVLHRMQEEGKINTTEETAARKAPLGLHVQYPRNDLAPYF
                     FEEIRKYLESTYGTEAVHERGLRVYTTLNIAMQKAANQAIRDGLH"
BASE COUNT          671 a          870 c         1023 g          776 t
ORIGIN      
        1 cggttcgtta atgatgcgga cgacttcgag gcccgcgatt tcgccggctt gcttggtggc
       61 ctggcgctgc gcgtcgttga agtaggccgg caccgtgatg acggccttgg tgactttttc
      121 gccgaggaaa tcttcggcgg cgttcttcag cttggtcaga atcatggcgg agatttcggg
      181 cgggctgtag attttgccgg aaatttccac gcgagcgtca tcgtgatcgc ccttcactac
      241 tttgtacgga acgcgcttgg tttccgagcc gacttcgtcg aagcggcggc ccataaagcg
      301 cttgatggaa tacaccgtgt tttccgggtt ggtgacggcc tggcgcttgg cgacctggcc
      361 gaccagccgc tcaccggttt tggtgatggc gacgacggac ggggtggtgc gtgccccttc
      421 ctgattgggg atgaccaccg gttcgccgcc ctgcataacg gagacgaccg agttggtggt
      481 tcccaagtcg attccgataa tcttactcat gctgtgtttc ctctcttgtt ggagaccaaa
      541 atctgctgcg cgaacagatc ctcggcggag cctcagacgg gcagctcaga tgccaggagt
      601 cgcgatgccc agttcgatgc aggagggagt ataaaggttg agtgcgtctt tgtcaagatt
      661 cctgatgtgt acaataagca ctgtattttc tgtttgttac ggccttcttg ttgagtaagg
      721 acggaccttg cgcggccggg ttcgcagaac aaaccctcgc ccgctttccg catcctactt
      781 ggcggcgtag agaaggcgcc gcgggtcggc tccttacttt ttccaggccg gcccgctctc
      841 ctttcacaga atccaaagga ttgagctaga atcgggcaag ctttttgagc acaaggagaa
      901 tcctgtgaag ggccgccacc tggcagctat cgcgatagcg ctcgttgtag ttggttcgct
      961 cctatttttc ggcgtgagcg gcaggcagga cccggtcgcg aatcaggccg ccgcgattcc
     1021 tcctaaagta gaagccgctt ctttgcctgc cgtgactcct ccggccgcag cgccttccgc
     1081 ctcggaactc acatcgccgc cggcgacggc tgccgccttg caacctgcaa ctcctgcgca
     1141 aggttccggc gttacggacg tcgatcctcg gcgcgcttat ggatcgaaga atgcgccgat
     1201 ttcgctggaa gtgttcagcg actttcagtg tccggcatgc aagcagctct ataaaaccac
     1261 caaccaactc ttgctggaca actacgtcaa caccaataag gtttatctga ttcaccgcga
     1321 ttttccattg ccgatgcacg cttattcgaa aatcgcggcg cgctatgtgc gggcagcgtc
     1381 gcaattgggt aagggagagc aggccgaaaa ggcgctgttt gataatcagg agaagtggga
     1441 acagagcgga gatgtggacg gaacactggc cggggcgctg agcgcggcgg aaatgaccaa
     1501 gatccgggcg gtagcgaagg gcacttcgct ggatgcggcg attgagaagg atgtggcgct
     1561 cgggaagggc tacaacgtga atcagacacc gacttcggtg atccacttta aagggcagac
     1621 ctatcccgtg gtgggcgtgg tcagctacga cgtgctgcat tcgtttctgg accagctgct
     1681 ttcgcaaaag taagcgtaaa gcctgcaaac aacagccact atgatgcaat ccagtccatt
     1741 gaacttccgg cgcgccgtca tttggctggg acgtttgctg attggcggaa tttttgttta
     1801 cgcgggttac gcaaagctgg tctatcccaa ccacaatttg tggccgtggt tcatgctgaa
     1861 gttttcggtt tcggcgaacc tttcgacgtt tgcgtttcag gtggagtcgt acaaaattct
     1921 gggagcggcc gcgtcctcgt ttgtggcgca cacgctgccg tttgtggaga ttgtgctggg
     1981 gttgctgctt ctggtcggat ggcgtttgcg gatctgggcg acgatcgtca gcgcgattct
     2041 ggtggggttt ctgggagccg taacgcgcgc ttacttgctg cacatggaca ttaattgcgg
     2101 gtgtttcggc acgccggagc ccctgacaat catgacagta cttcgtgaca gcgcgttgct
     2161 attgctggcg ctggtgatga cggtattcgc ttaccaagaa gcccgcacgg cgcatccgtg
     2221 gacggcagcg acaaattcgt agcccaagca aaagcagatt cctcacttgg gtcggaatga
     2281 ccatatgcag ttaagggtga caatgtggag ggtttggaaa cgccatggtc tgatcagccc
     2341 ctgcattcac ggtacatgcg ataaaatttg gtttcgatgc tgagtctgcc accgatcaca
     2401 ttgcggctgc gcgggtggaa gctgattgac cgcgtggcgc tggccttcct gctgctttgc
     2461 tccattgctc tgggcgctgc gtgcggattg ctgtttgtgt atgccagcga tttgccggaa
     2521 attcgcgcgc tggagacgta ccgtccgaac gtggtgacgg aaatttatgc cgacgacgga
     2581 cagatggttg gcagctttgc gctgcagcgg cgcattttga tgacctacga gcaatgcccc
     2641 aagattctgt tcaaagctgt gacctcgatt gaagaccagc attttgagga tcactgggga
     2701 atcgattttc cgcgcattgc gggtgctgcg taccggaatt tgctgaaggg ccgtaaagcg
     2761 gaaggcgcca gcacgattac gatgcagctg gcgggcaatc tttttctgga ccgcagcgac
     2821 cgcagcttcc gtcgcaagat gcaggagatg ctgctgtcgc tgcagattga gcggcggtat
     2881 accaagccgc agatttttgc gatgtacgcc aaccaggttt atttggcgca tggcaattat
     2941 gggttcgcgg cggccgcgca attttatttc ggcaaaccgg tgacggattt gaagctgcag
     3001 gaagcggcac tgctggccgg aatggtgaac gggccgaaat tttcaccgct cctgaatccg
     3061 gagctggcgc tttcaaggcg aaatctggtg ctgcaccgga tgcaagaaga gggaaagatc
     3121 aataccacgg aagagacagc ggcgcggaag gcgccactgg gcttgcatgt gcagtacccc
     3181 cgcaatgatt tggcaccgta tttcttcgaa gagatccgca aatatctgga gtctacttac
     3241 ggaacggaag cggtacacga acgcggcctg cgtgtgtata cgacgctaaa catcgcaatg
     3301 cagaaggcag ccaatcaggc gattcgcgac gggctacaca
//
LOCUS       DASTPM010000390         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_994186, whole genome shotgun sequence.
ACCESSION   DASTPM010000390 DASTPM010000000
VERSION     DASTPM010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994186"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1851
                     /locus_tag="VFR42_06835"
     CDS             37..1851
                     /locus_tag="VFR42_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0368905.1"
                     /translation="MATSVVTKAPAKGGSFLLESPAPQDVFTPADLTDDQKLIGQTAE
                     EFVLKEVLPLAKDLENKKPGLMAELVRKGGELGLMGGGIPEEYGGAGLDKIATTVLTE
                     KLSIYGGFAVTHGAHAGIGTLPIVYFGTEQQKKKYLPKLATGELIGAYCLSEPQAGSD
                     AQNSLTRAVLNAEGTHYVLNGQKMWITNGGFADLYIVFAKIDGEKFSAFIVERAYPGC
                     KPGNEEHKMGIRGSSTTPVFLENCEVPKENLLHEIGRGHIVAFNILNAGRFTLGASCV
                     GGSKYVLMTAAKYARERKAFGKSIGDFGLMKEKLAEMAIQIFAVESMVYRSAGNIQAM
                     VAAGTGDPSQGGASKTVHLMKVLEEYAIESSIAKVYGSEMLDFVVDEGVQIFGGYGFH
                     EEYPVCRAYRDSRINRIFEGTNEINRMLIVQMLMKRATGGQLALIPAAMKLADEILAG
                     PSFEETPEGALAEETRVVSNAKKMFLQAAGGAVQKFREKVADEQELVAALANIVMEVY
                     AMESCLLRAQKAAEAKGEATNAVMIAAARVFISDAMERAEHEAKRALASVHEGDMLTT
                     QMAVLKRFGKRAAVDTIALRRQVAAAVQSQDRYPFEGR"
BASE COUNT          482 a          510 c          692 g          401 t
ORIGIN      
        1 ggccgaaggt ttccggctcg cgtccggagg gatccgatgg caaccagtgt tgtgaccaaa
       61 gcgcccgcaa aaggcggcag ttttctgctg gaatctccgg cgccgcagga cgtttttact
      121 ccggccgatt tgaccgacga ccaaaaactg atcggacaaa ccgcggagga atttgtgctg
      181 aaagaagtct tgccgctggc gaaagatctg gaaaacaaga agcccggctt gatggcggaa
      241 ctggtgcgca agggcggcga actgggcctg atgggcggag gcattccgga agaatacggc
      301 ggagcgggtc tcgacaaaat cgcgacgaca gttttgacgg aaaagctttc gatctacggc
      361 ggatttgccg tcacacacgg cgcgcacgcc ggcatcggta ccctgccgat cgtgtatttc
      421 ggaacggagc agcagaagaa aaagtatttg ccgaagctgg cgacgggcga actgatcggc
      481 gcgtactgtc tgagcgagcc gcaagccggc tccgatgcgc agaattcgct gacgcgcgcg
      541 gtgctgaacg ccgaaggcac gcactacgtt ttgaacgggc agaagatgtg gatcaccaac
      601 ggcggcttcg cggatctgta catcgtgttc gcgaagattg atggcgaaaa gttttctgcg
      661 ttcatcgtgg agcgtgcata tcccggctgc aagccgggga atgaagaaca caagatggga
      721 attcgcggca gctcaaccac tccggtgttc ctggagaatt gcgaagtacc gaaagaaaat
      781 ctgttgcacg aaatcgggcg cgggcacatt gtggcgttca acattttgaa tgcggggcgc
      841 ttcacgctcg gcgcgagctg cgtgggcgga tcgaaatacg tgttgatgac cgccgcgaaa
      901 tacgcgagag aacgcaaggc attcggaaaa tcgatcggcg atttcggctt gatgaaagaa
      961 aaactggcgg aaatggccat ccagattttt gcagtggagt ccatggtgta ccgcagcgcg
     1021 ggaaatattc aggcgatggt ggctgcggga acgggagacc cctcgcaggg cggggcaagc
     1081 aaaaccgtgc acctgatgaa agtgttggaa gagtacgcca ttgaaagttc catcgcaaaa
     1141 gtttacggca gcgagatgct ggactttgtg gtggacgaag gcgtgcagat ttttggcggc
     1201 tacggtttcc acgaagagta tccggtgtgt cgcgcgtatc gcgattcgcg catcaaccgc
     1261 attttcgaag gcaccaacga aatcaaccgc atgctgatcg tgcagatgct gatgaagcgc
     1321 gcgacgggcg gtcagttggc gctgattcca gcggcgatga aactggccga cgaaattctg
     1381 gccggtccgt cgtttgaaga aaccccggaa ggagcgctgg ccgaagaaac gcgcgtggtg
     1441 tccaacgcga aaaaaatgtt tctgcaggcg gcgggcggcg cagtgcaaaa attccgcgag
     1501 aaggtggccg acgaacagga actggtcgcg gcgctggcca acatcgtgat ggaagtgtac
     1561 gcgatggagt cctgcctgct gcgcgcgcag aaagccgccg aagcgaaagg cgaggcaacg
     1621 aatgcggtga tgattgcggc ggcgcgggtg tttatcagcg atgcgatgga acgcgcggag
     1681 catgaagcga agcgggcgct tgcctcggtt cacgaaggcg acatgctgac gacacagatg
     1741 gcggtgttga agcgtttcgg caagcgcgct gcggtggata cgatcgcgtt gcgacgccag
     1801 gtggctgcgg cggtgcagag ccaggaccgg tatccgttcg agggccggta attgcagtgg
     1861 cgacgcgcat gtggctggag taagacgtgc cgtttttcag tcccccagtc gtgagtcttc
     1921 cgtcagagaa gagacgtgcc agagcgcgcg gcgagcaggt taaccgtaac cacctgtttt
     1981 ttcataagtg ctgattctaa gggacttaag tcacccgttt tcctacactt ttgcaattgt
     2041 gattctaaaa gtcttaccga agggcgttgt ttcggtggat tcagc
//
LOCUS       DASTPM010000391         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_792570, whole genome shotgun sequence.
ACCESSION   DASTPM010000391 DASTPM010000000
VERSION     DASTPM010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792570"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1935
                     /gene="polX"
                     /locus_tag="VFR42_06840"
     CDS             25..1935
                     /gene="polX"
                     /locus_tag="VFR42_06840"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEU0368906.1"
                     /translation="MSKNPRPAGKNFPAGPFLLRPHARGKNFGLWAFFQSGIPAYNRA
                     MDNQQIARILRETAQLLELDGAIIGRYRSYEKAAELISGLPESVEQMAKTPERLKELP
                     GIGDRMVEHIDEILKSGDYSLRKKLLKKYPATLLDVLQLQSLGPKKVALLWKQFKAGS
                     VSDVEKLAQDGKLRDLAGFGEKSEQNILKATAAFKRSTGRFHLNTAEASAEALRAHIE
                     KDGKTAESVTPAGSLRRGRETVGDLDLLVTLADGHTSKKHVDALAEHILKFPGIEQTL
                     AHGENKVSFTLRDGLQVDVRLLKKENFGAALLYFTGSKEHNVRLRGRANDMGWTLNEY
                     QLATLKGEKHVAGRTEEEIYAKLKLDYIAPELRENTGEIEAAEQHKLPKLIESGDIQG
                     DLQMHSTASDGKNTVEEMALAAKALGHTYIAMTDHSKAVTVANGLDEKRMAAQIKMLR
                     EVDKKGLGIRVFVGSEVDILKNGTLDYSDELLAQIDVVVCSVHSYMNLEAAEMTERLL
                     AAIENPYTQIIAHPTGRLLLRREAYPYDMEKVLDACAKHGVAMECNSYPDRLDLKDVY
                     LRMCKERGVKVVISTDSHSTGNLAYIKYGVLTARRGWLTKADVLNTLPPEEFLAALRP
                     KPGMAKRAGRSK"
     gene            2033..2944
                     /locus_tag="VFR42_06845"
     CDS             2033..2944
                     /locus_tag="VFR42_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU0368907.1"
                     /translation="MPKTEVKTTMNLLPFAGGRVAKLAGVGTLALAAMAFTAGIAQAE
                     EWTKSYTVSGRAHVRVDSNDGAVRVTTGDSKQIELRVTYSGYTLDKNLTINSRQDGDQ
                     VEFSARVRNSMNWGWGGIHRTLRLEVHMPREADLNVDTGDGSVEAQALAGNLNIHTGD
                     GHIRVDGAKGDIRLRTGDGSIEGRDLDGRLEADTGDGHVTVEGRFDSLNIKTGDGSIT
                     ARAGAGSKLANGWTIHTGDGSVDMSIPGDLQANIEASTNDGRISLGIPVTVEGTFSTS
                     KISGKMNGGGQPLTIHTGDGSIHLSKT"
     gene            complement(3375..3779)
                     /locus_tag="VFR42_06850"
     CDS             complement(3375..3779)
                     /locus_tag="VFR42_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368908.1"
                     /translation="MNEAAQKSTGRSIWAVVAGFVVVVVLSTVTDAVLHAAGLFPALG
                     KSMSNSLFALATAYRTLYGILGSYATARLAPNKPMKHSLIGAAIGTAIATIGAMATWN
                     MNLGPHWYPVALILTALPTGWLGAKLRMLQTQ"
     gene            4030..>4266
                     /locus_tag="VFR42_06855"
     CDS             4030..>4266
                     /locus_tag="VFR42_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU0368909.1"
                     /translation="MEKPAKIASPKGKLGVLLPGMGAVSTTFMAGVELVRKRLAQPVG
                     SLTQLGTIRLGKRTDNNSPLINKFIPLAGLDDLVF"
BASE COUNT          961 a         1044 c         1452 g          809 t
ORIGIN      
        1 gcaatcatgt cttagctagg gtttgtgagc aaaaacccta ggccggcggg gaagaacttc
       61 cctgccggcc cttttctttt gcggccgcac gcacgcggca agaatttcgg cttgtgggca
      121 ttttttcaga gcggcattcc cgcctataat cgcgccatgg acaaccaaca aattgcccgc
      181 atcttgcggg agacggcgca gctattagag ctcgatggag cgattatcgg gcgctaccgg
      241 agttatgaga aggcggcgga actgatttcg ggattgccgg agtcggtcga gcagatggcc
      301 aagacaccag agcggttgaa agagctgccg gggatcggcg accggatggt cgagcacatt
      361 gacgagatcc tgaagagcgg ggactattcg ctgcggaaaa aattgttaaa gaaatatccg
      421 gcgacgctgc tggacgtgct gcagctgcaa tctctggggc cgaagaaggt ggcactgctg
      481 tggaagcagt tcaaggccgg ctccgtgtcg gatgtggaga aactggcgca agatggcaag
      541 ttgcgcgacc tggcgggctt cggcgaaaaa agcgagcaga atattttgaa ggcgacggcg
      601 gcgttcaaga ggtcgacggg aagatttcat ctgaatacgg cggaagcttc ggcggaggct
      661 ttgcgggcgc acattgaaaa ggacggaaaa accgcggaga gcgtgacacc ggcgggttcg
      721 ctgcggcgcg ggcgagaaac ggtgggcgat ttagacctgt tggtaacgct ggcggacggg
      781 cacacttcca aaaagcatgt ggacgcgctg gcggaacata ttttgaagtt tccgggaatt
      841 gagcagacgc tggcgcacgg cgaaaacaaa gtgagcttca cgctgcggga cgggctgcag
      901 gtggatgtac ggctgctgaa gaaggaaaat tttggcgcgg cgttgctgta ctttacggga
      961 tcgaaagagc acaacgtgcg gctgcgcggg cgcgctaacg atatgggatg gacgctgaat
     1021 gagtatcagc tggcgacgct caaaggcgaa aaacacgtag ccgggcggac cgaagaagaa
     1081 atttacgcca agctgaagct ggattacatt gcgccggagt tgcgggagaa caccggagaa
     1141 atcgaagctg ccgaacagca taagctgccg aagctgatcg aaagcgggga catccagggc
     1201 gatttgcaga tgcactcgac ggccagcgac gggaaaaata ccgtggaaga aatggcgctg
     1261 gcggcgaaag cgctgggaca cacatacatc gcgatgacgg atcattcgaa agcggtgacg
     1321 gtggcaaacg ggctggatga aaagcgcatg gccgcgcaga tcaagatgct gcgcgaggtg
     1381 gacaagaagg ggctggggat ccgcgtattt gtgggatcgg aagtggatat cctgaagaac
     1441 gggacgctgg attattcgga cgagttgctg gcgcagattg acgtggtggt gtgctcggtg
     1501 cattcgtata tgaatctgga agcggcggag atgactgagc ggctgctggc ggcgattgag
     1561 aatccttata cgcaaatcat cgcgcacccc accggacggc tgttgctgcg gcgcgaggcg
     1621 tatccgtacg acatggaaaa ggtgctggat gcgtgcgcga aacatggcgt ggcgatggag
     1681 tgcaattcgt atccggaccg gctggacctg aaagatgtgt acttgcggat gtgcaaggaa
     1741 cgcggggtaa aggtggtgat ttcgacggac tcgcacagca ccggcaacct ggcgtacatc
     1801 aaatacggtg tgctgacggc gcgccggggg tggctgacca aggctgacgt gctgaatacc
     1861 ctgccgccgg aggagtttct ggcggcgctg cggcccaagc caggaatggc caaacgggct
     1921 ggacggagta agtgacatcg actctcgacc gccagtccga gttggcgcgg gtggccagcc
     1981 ggggaacatt ttgggcattg cgttcgttcc ataaggtagc aactactctg gaatgccaaa
     2041 aaccgaggta aagacaacta tgaaccttct tccctttgca ggtggccgcg tggccaagct
     2101 ggcaggggtc gggactctgg cgctggccgc aatggcgttt acggccggga tcgcacaggc
     2161 cgaggaatgg accaagtcgt atacggtcag tggccgggcg catgtccgcg tggacagcaa
     2221 tgatggcgct gtgcgcgtga caaccggcga cagcaagcag atcgagctgc gggtgaccta
     2281 cagcggctac acactggaca agaatttgac gatcaactcg cggcaggacg gcgaccaggt
     2341 ggagtttagc gcacgcgttc gcaacagcat gaactggggt tggggcggga tccaccggac
     2401 cttgaggctg gaagtgcata tgccgcgcga agcggacctg aacgtggata cgggcgatgg
     2461 gtcggtcgaa gcgcaggcgt tggcgggcaa tttgaacatc catactggcg acggacacat
     2521 tcgtgtcgat ggcgccaaag gcgacattcg gctgcgcacg ggcgacggca gcattgaggg
     2581 cagggatttg gacggcaggt tggaagccga taccggcgac ggccatgtga cggtggaagg
     2641 gcgtttcgat tcgctgaaca tcaagacggg cgacggcagc atcacggcgc gcgccggtgc
     2701 tggttccaag ctagctaacg gctggacgat tcatacgggc gatggcagcg tggatatgtc
     2761 gattccgggc gacttgcagg ccaacattga agccagcacc aatgacgggc ggatcagcct
     2821 gggcattccg gtgactgtcg aaggcacgtt cagcacgtcg aagatttcag gcaagatgaa
     2881 cgggggcggg cagccgctga cgattcatac gggcgacggc tcgattcatt tgagcaagac
     2941 ctagtttgcg atgcgagttg gtttcgaaca gacttaacga ttggccttgg tgcaagcagg
     3001 tcgaggaaaa ggcggggcgg agtgattttc cactccgctt ttttattgta gccagtccag
     3061 tggagtgcgc tcgctgagag aattcgttac gctgtcgtat tcggtttcag tactccacga
     3121 gtgtaggcag aatcgcactt ctgtcggcaa attcccgcgg tgtctcaaat cgttgcgctg
     3181 taactccttg aaaacaaagc caggcagttg gaatgagact tgcctttaag gatggcgttc
     3241 ggggaggtag tgcccttgat cgtatatgct ctgttactcg ccgtggcaac actgggcgcc
     3301 gtaacgctaa ccagttcgat tgtggaaacg caccggcgcc ggtcttcgta agcggaaact
     3361 tcgccacaaa tttttcactg cgtttgcagc atgcggagct tggctccaag ccagccggtg
     3421 ggcaaagccg tcaaaattaa ggccacgggg taccagtgtg ggccgaggtt catgttccac
     3481 gtagccatgg cgccgatggt cgcgatggcc gttccgatcg cggcgccgat cagcgaatgc
     3541 ttcatcggct tgttaggcgc gagccgcgcg gtcgcgtaac tgcccaaaat gccgtagagc
     3601 gtgcggtagg ctgtggctaa agcgaagagg ctgttgctca tgctcttgcc aagtgccgga
     3661 aagagcccgg cggcgtgtag cacagcgtcg gttacggtgg acaaaacgac gacgaccaca
     3721 aagcctgcga caactgccca aatgctacgc cccgtacttt tctgtgctgc ctcgttcatg
     3781 ggttgaaccc tccgtcgggt cgcgactgcg attggcgtga gatggtagtg gatgaacggc
     3841 ggccatgcca ctaggatttg cgccggctcg cggggtgacg cgccagccag cggaacgaag
     3901 cggaatccag gacgagaagc gcaatttgca tgggcgaagc ggtgtgcgcg atactatttg
     3961 tttgtgtagg ggcgtggatt cgcagctgta gcattcatcc gcattcatcg accgaaactg
     4021 aggggtggca tggaaaagcc agcaaagatt gcatcgccaa agggcaaact gggagtgctg
     4081 ctgcccggca tgggcgcggt gagcaccacg tttatggcgg gcgtggaact ggtgcgcaaa
     4141 cggcttgcgc agccggtcgg gtcgctgacg caactgggaa ccattcggct gggcaagcgc
     4201 accgacaaca attcgccgct gattaataaa ttcattccac tggctggact ggacgatttg
     4261 gtgttc
//
LOCUS       DASTPM010000392         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_100475, whole genome shotgun sequence.
ACCESSION   DASTPM010000392 DASTPM010000000
VERSION     DASTPM010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100475"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFR42_06860"
     CDS             <1..789
                     /locus_tag="VFR42_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU0368910.1"
                     /translation="DPEVEIRPVKGQVDDLLEQIRTRAEKNERVLVTTLTKRMAEDLS
                     EYFTEIGVRCRYLHSEVETLDRIKILRDLRRGEFDVLIGINLLREGLDLPEVSLVAIL
                     DADKEGYLRSATSLIQTIGRCARHVEGRAILYADTRTRSMSEAIAETNRRREKQFAYN
                     QENNITPQSIIKGVDMELAHIVEADYVTIPLDVGELDAAVAGAKSEEQVAQLLQKLEA
                     QMREAAKKVEFEKAAQLRDRIRSIKNQDLSGAFGRQVPDQAQAG"
     gene            complement(809..2002)
                     /locus_tag="VFR42_06865"
     CDS             complement(809..2002)
                     /locus_tag="VFR42_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0368911.1"
                     /translation="MLGYRVGGIEHQRMHLIGLVLFSFIFAFWILQGLRIARGALRLP
                     HLKDQGPCEPGDCPSVSLLFAARNEAEKLPQALATLEKIDYPALEIIAVNDRSTDATS
                     AILRGAAERDSRLKIVNVDNLPGGWLGKPHALQCGYEASKGDWLLFTDADVCFKPDSL
                     RRAMRMANLRGLDHLTLMGDVEMHGFWEKTVLSFFALGFYIGTNPAAVSDPNSRAYLG
                     VGAFQLLKRHAYETTGTHRRLAMEVVDDLRLGKIVKQAGFRSGVGLAGDYVSVRWHAG
                     LRNIVRGVTKNFFAVAGYRIGVVAFQLAGIFCTNVLPFVSLPFIDGWTRILAAASMAI
                     ALAAHAGTSRVMKASGWYALTQPLGAIIFGYMLLRSTIVTLKQGGVVWRGTFYPLKEL
                     RRGLV"
     gene            2001..2903
                     /locus_tag="VFR42_06870"
     CDS             2001..2903
                     /locus_tag="VFR42_06870"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEU0368912.1"
                     /translation="MVTRYGISIIGAGRLGRSLGRSLRERGWRIHSVVTRSQTTARRA
                     VRSIGAGHACAAISSEVFLAPVILIAVPDSSIQAVVQQLADVGAQDARKHFILHTSGA
                     LASDVLAPVRQLGASVGSLHPLQSFSGVGAPSLEGRVFAIEGDAGAVRVSRSLVRSLD
                     GQVLLLDSLAKPLYHTAASIAAGQVLAAIEAAIQLLASIGMKRREALRALLPLTRQVL
                     ENQERLGARAAWTGPLARGDFRVIAAHEKALLTRPEEYLAAYRALNRLAARVLARDPE
                     SVLAALDEISRESGPAPKAKGVSA"
BASE COUNT          659 a          974 c          822 g          613 t
ORIGIN      
        1 gatccggaag tcgagattcg tccggtcaag ggtcaggtgg atgacctgct cgaacagatt
       61 cgcactcgcg ccgagaaaaa cgaacgcgtc ctggtcacca cgcttaccaa gcgcatggca
      121 gaggacctca gcgaatactt caccgagatt ggcgtccgct gccgctattt gcattccgag
      181 gtcgaaacac tcgaccgcat caaaattctt cgcgacctgc gccgcggcga attcgacgtt
      241 cttatcggca tcaacctgct tcgtgaaggg ctcgatttgc cggaagtttc gctggttgcg
      301 attctcgacg ccgataaaga gggatacctg cgcagcgcca catctttgat tcagacgatc
      361 ggccgctgcg ctcgccacgt cgaaggccgc gccatacttt acgcggacac tcgcactcgt
      421 tccatgagcg aggccattgc cgaaaccaat cgccgccgcg aaaagcaatt cgcctacaac
      481 caggaaaaca acatcacgcc gcagtctatc atcaagggcg tggatatgga gctggcgcac
      541 atcgtcgagg ccgactacgt tacgatcccc ctcgacgtcg gtgagctcga tgccgccgtg
      601 gcgggcgcga aatccgagga acaagtcgcc caactgcttc agaaactcga agctcaaatg
      661 cgagaggccg ccaagaaagt cgaattcgag aaagccgcgc agcttcgcga tcgcattcgt
      721 tccatcaaaa accaggattt gtcgggcgct tttggccgcc aggttccgga tcaggcgcag
      781 gccggataga ctgccgaccg cagagacgtc aaaccaatcc tcggcgcagc tctttcagcg
      841 ggtaaaacgt gccgcgccac accacgccgc cctgcttaag cgtcacaatg gtcgatcgca
      901 gcagcatata gccgaaaatg attgcgccaa ggggttgcgt caaggcgtac caaccggacg
      961 ctttcatgac tcgggatgtt cccgcgtgtg cggcaagggc gatcgccatg ctcgctgcag
     1021 ctagaatccg cgtccatccg tcaatgaatg gcaacgacac aaaaggcagc acattcgtac
     1081 aaaagattcc cgccaactgg aacgcaacga ctccaatccg gtagcccgcg accgcgaaga
     1141 aattcttggt cacgccccgc acaatgtttc gcaatcccgc atgccaccgc acgctcacgt
     1201 agtcgccggc caatcccacg cccgaccgga atcctgcttg cttcacgatt ttgcccagcc
     1261 ttaggtcgtc tactacttcc atcgccagcc gccgatgtgt tccggtcgtt tcatacgcat
     1321 ggcgcttcag caattggaac gcgcctacgc caagataagc acgtgaattc ggatcggaca
     1381 ccgcagcagg attcgtcccg atgtaaaacc cgagcgcaaa gaagctcagc accgtcttct
     1441 cccaaaatcc gtgcatttcc acgtcgccca tcagagttaa gtgatccaga cctcgcaaat
     1501 tcgccatccg catagctctt cgcaatgagt ccggcttgaa acaaacatcg gcatcggtaa
     1561 aaagcagcca atctcctttg gaagcttcat acccgcattg caatgcgtga ggtttgccga
     1621 gccaacctcc cggcaaattg tcgacattta ctattttgag tcgactatca cgttccgctg
     1681 caccgcgaag gatcgccgaa gtcgcatcgg tcgaccggtc gtttaccgcg ataatttcca
     1741 aggccggata gtcaattttt tcgagcgttg ccaatgcctg cggaagtttc tctgcctcat
     1801 tccgtgcggc aaataagagc gagacgctcg gacaatcgcc aggttcgcac ggcccttggt
     1861 ccttcaggtg cggtaagcgt aatgctcccc gcgcgattcg cagaccctgc aagatccaaa
     1921 acgcgaagat gaaagaaaaa aggaccagcc cgatgaggtg catgcgttgg tgttcgatgc
     1981 cgccgacacg gtatcctaac atggtgacgc gctacggaat ctccatcatc ggagcaggac
     2041 gactcggacg ctcgttgggc cgcagcctcc gcgaacgggg ctggagaatt cattccgtgg
     2101 taacgcgaag tcaaaccacg gcacgccgcg ccgtgcgctc catcggcgcg ggccatgcgt
     2161 gcgcagcgat ctccagtgaa gtgttccttg cgcctgtcat tctgatcgcg gtgccggatt
     2221 catcgattca ggccgttgtg cagcaacttg ccgacgttgg cgcacaggac gctcgcaagc
     2281 actttatcct gcacaccagc ggtgcgctcg cttccgacgt gctggctccg gtgcgccagt
     2341 tgggtgcctc cgttggctct ttgcatccgc tgcaatcgtt tagcggcgtt ggtgctccta
     2401 gtcttgaggg ccgcgtcttc gccatcgaag gcgacgcagg agcggtccgc gtttcccgct
     2461 cgctggtccg gagtcttgat ggccaggttc tgctcttgga ctccctagcc aagcccctct
     2521 accacaccgc cgcttccatc gccgccggac aagtgctggc cgcaattgaa gctgccattc
     2581 aacttcttgc ttccatcgga atgaagcgcc gcgaagccct gcgcgccctc ttgcccctta
     2641 cccgccaggt gctcgaaaat caggagcggc tgggcgcccg tgcggcttgg accgggccgc
     2701 tggctcgcgg cgattttcgc gtaatcgcgg cacacgaaaa agccttactc acgcgtcccg
     2761 aggaatattt ggctgcttat cgtgccttga atcgcctggc agctcgcgta cttgcccgcg
     2821 atccagaaag cgtgctggcc gccctcgacg agatttcgcg ggaatccggt cccgccccaa
     2881 aagccaaagg agttagcgct tgaagatatt gcaaattccc atcgaacaca aaacgctcgc
     2941 caacggattg cacgtcgtgg tcgctcccga ttcgacagca ccggtcgtca cggttggcgt
     3001 ctactacaaa atcggctttc gcctcgaacc ccagggacgc agcggcttcg cccatctttt
     3061 cgagcaca
//
LOCUS       DASTPM010000393         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_590598, whole genome shotgun sequence.
ACCESSION   DASTPM010000393 DASTPM010000000
VERSION     DASTPM010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590598"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFR42_06875"
     CDS             complement(<1..449)
                     /locus_tag="VFR42_06875"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU0368913.1"
                     /translation="MAEASQRHSFLVRVTHWLTTLAFLALLVSGLELVVSHPRFYWGE
                     TGNVNTQPLFHIPIPASRATVPTGYGYTLPDQNGWSRYLHFQSAWLLVLTGLVYVGVG
                     AFRGHFRRELFPTRSDLSAKQLMRSIGEHLRFRRPGAEEAWSYNVLQR"
     gene            complement(1026..1520)
                     /locus_tag="VFR42_06880"
     CDS             complement(1026..1520)
                     /locus_tag="VFR42_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEU0368914.1"
                     /translation="MRRAVLVAILTAGGLVGGCGVGVHLGESGEAAELRKLDEQWSAA
                     AAKNDLEATLSFYADDAVVLPGNAPIAADKKAIREVWAGMLGPGTAVSWKVTKVEVAK
                     SGELGYLYGNYELTMKDPKGGPPDQDKGKLVEIWRKQPDGKWKCIVDTYNSDLPLPAA
                     AAQK"
     gene            1667..2536
                     /locus_tag="VFR42_06885"
     CDS             1667..2536
                     /locus_tag="VFR42_06885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0368915.1"
                     /translation="MQLNQDVINQWSSAAKFWEKHRAVVKQMFSPVTDALVEDAQIAS
                     RHAASSLGRVLDIATGPGDPALSVAPLLGPEGRVFGVDPIPEMVAAARRAANDFGFSN
                     AQFEIAFADHLPFPADTFDAIISRFGAMFFPSPVDAVREMLRVLKPGRKLALAVWHSA
                     DTNPFFNTVSQVIDRYVESPAPAPDAPDTFRFATPGQLRDVLVESGVMAPAERLLQFH
                     IQAPVSAEEFWTLRCELSEKMREKIGRLSSEQLTEVKRQVLEAFQPYCTASGMTFPAQ
                     VLIISGTKPPATN"
     gene            2593..3075
                     /locus_tag="VFR42_06890"
     CDS             2593..3075
                     /locus_tag="VFR42_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0368916.1"
                     /translation="MAANARLRDSRYTFRRIASAANKDFNFGAVSRSVLIADDSASMR
                     RSVRFLLERHRIELRISEAVDGADAIEKARGTKPDLILLDLAMPRLNGVEAASILQGI
                     LPNTPIILFTMHTDLHADSLSAFIKVDFVSKVDGVSRLLERVDTLLPPIVSDDGVALH
                     "
BASE COUNT          814 a         1174 c          913 g          829 t
ORIGIN      
        1 cgctgcagca cgttgtaaga ccaggcctct tccgcgcccg gcctcctgaa acgcaaatgc
       61 tcgccgatcg agcgcatcag ctgcttggcc gaaaggtcag accgtgtggg aaacaactcg
      121 cgccggaaat gtcctcggaa cgctcccacg ccgacgtaaa ccaggcccgt caggaccaac
      181 agccacgcgg attgaaaatg cagatagcgg ctccagccat tttggtctgg cagcgtgtag
      241 ccatacccgg tcggcaccgt agccctggag gccggaatcg gtatgtggaa taacggctgc
      301 gtgttgacgt tgccggtctc gccccaataa aagcgcggat gcgaaaccac cagctctagt
      361 ccactcacca gcagcgccaa aaacgcaagg gtagtcagcc aatgcgtgac gcgaaccaaa
      421 aaggagtgcc gctgggaagc ttccgccatt gtccgagttg tacgctttgc gcgctcctcg
      481 cgcaagacat atctgttgaa ccgcacgcct aatcaggggg aaccccgggg cgcggtgtaa
      541 cgctttcgcg tgaaagttcg gtgtggaagg cactttcggc cttctatttg aggtggtctt
      601 aggaatttta gatcatcttc agcgtgcgtt gagcccatga ttgtctgggg actctttcct
      661 gtagggctcg cacttcatct ccgattctat cgtccgctca aaggcatcca aaaaaagaaa
      721 atcctgggat gattgcacta gcgttggctt tttctctcat tggttttgga tatttggaag
      781 actaagagat tagggaaaaa acgcaaaagc aagaagtgcc cgaccataaa tgcgaactta
      841 agacagatcc agaaaacaaa cccaccaaag aagaatctgc agaaaggtcg tccaagccac
      901 ctaaaaaagc gcctcttgcg acgaacaacg ccgacatgaa gaagcaatca ccggaccagc
      961 ttgcgtactt gttggcacag tgccatctga tgattccaac tgtcgattgt tgacctcttc
     1021 gcgagtcatt tctgtgccgc agccgccggc aacggcaaat ccgaattgta cgtatccaca
     1081 atgcacttcc acttcccgtc gggctgcttc ctccagatct ccaccagttt gcccttatcc
     1141 tggtccggcg gaccgccctt cgggtccttc atcgtcaatt cataattccc gtagagatag
     1201 cccaattcgc ccgacttcgc cacctcaacc ttcgtcactt tccaagacac cgccgtcccc
     1261 ggcccgagca tccccgccca cacctcccgg atcgccttct tgtccgcagc gatcggtgcg
     1321 ttcccgggca gcaccacggc atcatccgca taaaacgaca gcgttgcctc caggtcattc
     1381 ttcgctgccg cggccgacca ctgctcatcc agcttccgca gttccgccgc ctccccagac
     1441 tcgccgagat gcactcccac accgcagccg ccaactaggc cgcccgccgt aaggatcgcg
     1501 accaacactg ctctccgcat aaacgctcct cctcgttgcg acttcgatgg aagtttctgc
     1561 aatcaacttg taccacctat gctactcatc ttgccacccg gcctagaatt gcggactttg
     1621 tcctgaaaac ttccgcacgc ttcaagcgaa cggtatactt tcgctcatgc aactcaacca
     1681 agacgtcatc aatcaatgga gctctgccgc taagttctgg gagaaacacc gcgctgtcgt
     1741 caaacagatg ttttcgccgg taaccgacgc cttggtcgag gatgcacaga ttgccagccg
     1801 acacgccgct tcgagtttag gacgtgttct ggacattgcc accggccccg gagatccagc
     1861 actaagcgtg gctcctttgc tcggaccgga aggcagggtc tttggagtcg atccgattcc
     1921 ggaaatggtc gcagccgctc gccgggcagc aaacgatttc ggtttcagca atgcgcaatt
     1981 tgagattgcc tttgcagacc atctgccgtt ccccgcggat accttcgacg ctatcatcag
     2041 ccgtttcggc gcaatgttct ttccgtctcc ggttgacgcg gttcgtgaga tgttgcgcgt
     2101 actcaagcca gggcgcaaac tggccctggc cgtttggcac tcagccgaca caaatccctt
     2161 tttcaatacc gtgtcacaag tcatcgatcg ctacgtcgag tcgccggcgc ccgccccgga
     2221 cgctcccgac acattccgct ttgccacgcc aggccagtta cgcgacgttc ttgtcgaatc
     2281 tggcgtgatg gctccggcag agcgcctgct gcagttccat atacaagcgc cagtctccgc
     2341 ggaagaattt tggaccctgc gctgcgaact ctccgaaaag atgcgcgaaa agatcggccg
     2401 cctttccagc gaacagttga ccgaagtgaa gcgacaggta ctagaagcct ttcagcctta
     2461 ctgcacagcc agcggcatga ctttcccggc tcaggtcctc atcatcagcg gaacaaaacc
     2521 gcccgcgaca aattgaacca cacgccgagg aaacgttatc cagcatccag tcctatcaac
     2581 ctcttcgagc ccatggcggc caacgcgcgt ctacgtgatt cccgctacac attccgccgt
     2641 attgcatcag cggcaaataa agattttaat ttcggcgccg tgtctcgaag tgtgctgatc
     2701 gcggatgaca gcgcatccat gcgtcgttct gtccggttct tgctcgaacg ccaccggata
     2761 gaacttcgaa taagcgaagc cgttgatggt gccgacgcca ttgaaaaagc gcgtggcaca
     2821 aaacccgatt tgatccttct cgacctcgcc atgcctcgtc tgaacggcgt cgaagcagct
     2881 tcaatcctgc aaggtatcct gccaaacact cccatcattc tgttcacaat gcataccgac
     2941 ctccacgccg attccctctc cgccttcatc aaggttgact ttgtttcaaa agtggatggc
     3001 gtttcgcgct tgctcgaacg tgtcgacacc ttgctgccac caatcgtctc cgacgatgga
     3061 gtggctctcc attagcacgg gcgggtggct agtccaaatt ttggagcccg ctcatcacct
     3121 tcgatattta aattagataa ccattaacta tatactaaat ctgggctact cttttttgtg
     3181 aagagtggct caaatccgcc aagtcttttc ctgccgcatg tggggaaatc gcctcctaag
     3241 tccccctgcc atacctacaa aaaacggttt gtaagtcgtc gcttcgaagg ttttctggtt
     3301 gcggtcgcgt ccgctactgc cacatgtccg aaaaaaaagt aggggtaccc gcccgcattt
     3361 ccgtgccaac gactccatga cctggcgtct gcgaacctag cttcgtaggt cggagacctc
     3421 agctccctgc cctgcattgt ttcattttgg aattgccctg ctctaccgga ggcgtcaacc
     3481 aagcggctcg accagcggtc aatcgcggcg cgctggcggc ttacaaatcc ctggctcctg
     3541 atccctaatc cctgccccct acatcttata ccggcccatg tcgtcgtcag aaaggccttc
     3601 gagccagttg cgcagctgtt cgttggtggt gcgttcggtt agcgcgctgg ataccttgga
     3661 gttcttcagc acctgctcgt ccacgaagat cgggcaatcc atgcgcagag ccagcgccag
     3721 cgcgtcgctc
//
LOCUS       DASTPM010000394         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_446537, whole genome shotgun sequence.
ACCESSION   DASTPM010000394 DASTPM010000000
VERSION     DASTPM010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446537"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFR42_06895"
     CDS             <1..195
                     /locus_tag="VFR42_06895"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0368917.1"
                     /translation="SLVRLQQKGWISAEWGTSDNNRKAKFYAITRSGRKQLSQDAAYW
                     QRLTGVMGRVLALRIGGDHK"
     gene            192..>2140
                     /locus_tag="VFR42_06900"
     CDS             192..>2140
                     /locus_tag="VFR42_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368918.1"
                     /translation="MIARLRQSAHRFLAIFRPSTLDRELDAEIATHLQFAIEENLQRG
                     LTPAEARRQALVQFGGPQQSRERHRDTRSFSFLDEILQDLRYTLRILRKDHSFTVIAL
                     IILALAIGANTAVFSVVNTLLLRPLPFPDSHQLAWIAPPPSGCGLSCATYSADAYEEF
                     RDQSFAYQGVTGYGAFSTPENLRLTGRGEPQPATGIEVIGNFFQVLGVQPAKGRLFTP
                     DEARGGPQRVALLADPYWRRQFNADSDIVGKSIELDGTLVTVVGVLPRSFDFGAVFSP
                     GAKVDLFTPLDLNQQRNWGNIVTLIGRLKPGVTTTQALQDAERVATNMYFNTRYPETK
                     GRYKGRLVPVTLKDYVTGKLNRSLIALWASVGVILLIAGVNLANLLLARTAARGKEFA
                     LRGALGANRGRIIRQLLLESLVLSGAGAALGLALAFALITWLKTQGSLALPLLSTLRI
                     DAQALSWTAFIALITAMIFGLLPGLRMASGNLQDSLKDSGPGAGLGRKQERLRGALVI
                     SEVALACMLLVSAGLLLRSFMKVLDVDLGFEPDRAASIKIDYDDSAPTEEASQTKRGV
                     IFQQILERVTALPGVEAAGMADYLPLGPNREWDTPVPQGKTFAPGELPDPLVYIVTPG
                     FVHAMGTRLRGRDFSWSDGPRSERVV"
BASE COUNT          395 a          729 c          558 g          458 t
ORIGIN      
        1 tccctggtgc gcctccagca gaaaggctgg atctccgcag agtggggcac ttccgacaac
       61 aaccgcaaag ccaagtttta tgccatcacc cggagcggcc ggaaacagct gtctcaagat
      121 gccgcgtact ggcagcgtct caccggcgtc atgggccgcg tcctcgctct gcggatcgga
      181 ggcgaccaca aatgattgcc cgcctccgtc aatccgctca tagatttctc gcgatctttc
      241 gtccatccac tctagatcgc gagctcgacg ccgaaatcgc cacgcacctc caattcgcca
      301 tcgaagaaaa tctccagcgc ggactcaccc ccgccgaagc ccgccgccag gccctggtgc
      361 aatttggcgg cccgcagcaa tcccgcgaac gccaccgcga cacccgcagt ttttcctttc
      421 tggatgaaat cctccaggac ctgcgttaca ccctgcgcat ccttcgcaaa gaccactcgt
      481 tcactgtcat agcgctcatc attctcgctc tcgccattgg cgccaatacc gccgtcttca
      541 gcgtcgtgaa tactctgctg cttcggcctt tgccttttcc ggactcccac caactcgctt
      601 ggatcgctcc gcctcccagc ggctgcggtc tctcctgcgc cacctattct gccgacgcct
      661 acgaagagtt ccgcgatcag agctttgctt accaaggcgt caccggatac ggagccttca
      721 gcactcctga aaatcttcga ctcaccggcc gtggcgaacc ccaacccgcc accggcattg
      781 aggtcatcgg caactttttc caggttctcg gtgtccagcc agccaaaggc cgcctgttca
      841 ctcccgatga agctcgcggc ggtccgcagc gcgtagcctt gctcgccgat ccttactggc
      901 gccgccagtt caacgctgat tccgacatcg tcggcaagag cattgaactg gacggaactc
      961 tggtaacggt tgtcggtgtc ctgccccgca gtttcgattt cggcgccgtg ttttccccag
     1021 gcgcgaaagt cgacctcttc actccgttgg atctcaacca gcagcgcaat tggggcaaca
     1081 tcgtcaccct gattggccgg cttaaaccgg gcgtcactac cacgcaagcc ctgcaggacg
     1141 ccgaacgcgt tgcaacgaat atgtatttca atacgcgtta tcccgaaacg aaagggcgct
     1201 ataagggaag actcgtaccg gtaacgctga aagattacgt gacgggaaaa ctcaaccgct
     1261 cgctgatcgc attgtgggct tccgtaggcg tcattttgct catcgccggc gtcaatctcg
     1321 ccaatttgtt gctggctcgc accgctgctc gcggcaaaga gttcgctctc cgcggagcgc
     1381 tcggcgcaaa ccgtggccgc atcattcgcc aattgctttt ggaaagcctg gtgctctccg
     1441 gcgctggcgc ggctctcggt ctggcattgg cgtttgcgct cattacctgg cttaagaccc
     1501 agggttcgct ggctctgccc cttctcagca cgttgcgcat tgacgcacag gctctcagtt
     1561 ggactgcctt catcgccctt attacagcga tgatcttcgg cttgcttccc ggcctcagga
     1621 tggccagcgg caatctgcaa gattcgctca aagactccgg cccgggtgcg ggcctcggcc
     1681 gcaagcaaga gcgtctccgc ggcgcgctgg tcatctctga agtcgccttg gcctgcatgc
     1741 tgctggtcag tgcaggactt ctcctgcgca gcttcatgaa ggtcctcgac gtagacctgg
     1801 gcttcgagcc tgatcgtgcc gcttccatca aaatcgacta cgacgatagc gctcccaccg
     1861 aggaggcgag ccagacgaaa cgcggcgtaa tctttcagca gattctcgag cgcgtcacgg
     1921 ctcttcccgg tgtcgaagca gccggcatgg ccgattacct tccccttgga ccgaaccgcg
     1981 agtgggacac tcctgtcccc caaggaaaaa cgttcgctcc cggcgaactt cccgatcctc
     2041 tcgtctacat tgtcactccc ggattcgtcc atgcgatggg cacgcgcctg cgcggccgcg
     2101 atttttcctg gtccgatgga cctcgcagcg aaagagtcgt
//
LOCUS       DASTPM010000395         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_331281, whole genome shotgun sequence.
ACCESSION   DASTPM010000395 DASTPM010000000
VERSION     DASTPM010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331281"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..763)
                     /gene="mtgA"
                     /locus_tag="VFR42_06905"
     CDS             complement(56..763)
                     /gene="mtgA"
                     /locus_tag="VFR42_06905"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEU0368919.1"
                     /translation="MTEKPDTHSKTSLRRDSLRFFVRWLAIGVVLLWSLAALILLAAR
                     WIDPPTTAVHIQRRLQAWTHPTPYHKRYKFTPLSQISPNLQHAVVAAEDARFYQHHGF
                     DWHQVQIAAEDDLEGGRTRGASTITQQLIKNLFFGTGRSMLRKGAEFTLVPVAEFVLG
                     KRRILEIYLNIVEWGPGIYGAESACRYYDGNAARNIGREQAARLAAILPAPLKRRPER
                     MNNYSARILVRMGQMGW"
     gene            complement(846..1331)
                     /locus_tag="VFR42_06910"
     CDS             complement(846..1331)
                     /locus_tag="VFR42_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0368920.1"
                     /translation="MKCLAVLIILLFAALPACADSISLHTFALQENPIRLEPLGSGLD
                     PLLVPVAIHGGQRGLVFDTSMDPIGSVVFSSTISLLGAQFTLGPIPIQCSATCAVGYG
                     FLLPISYKMVQGTLSLTLNGVTETYDFRYQSPAPEPGGVLLLGTGLVGIAWRKYAAGR
                     R"
     gene            complement(1575..>2794)
                     /locus_tag="VFR42_06915"
     CDS             complement(1575..>2794)
                     /locus_tag="VFR42_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368921.1"
                     /translation="GLTLWGLEAYLGQRWRAAVMWFSLAVLAKETAVLAPLGLLAWEM
                     VTYLLPFGPLKGLASTMSMPSPERCPDRKPFPRIQFASLSVPILVLCSWYGYHYARTG
                     YVFGNPEFFRYNVQATMSGVRIVLALGMRLWQSFGYMHLWVLMLLMMWAMTRPALRDG
                     DRERPRIAIPVQCLFAALILTYVAAMAVVGGAVLARYMLTVVPLVILVAVSTLWRRIR
                     QWRWLVGLACVAFVLALLFNPPYGFAPEDNLAYRDYIVMHREAESYLESHYPKARVLT
                     AWPASAEISQPYLGYVAQPLQVLRVEDFSADEILGAAQARSRFDVALVFSTKYEPHPT
                     LFDRWRAWESIKTRFFGFHRDVPPVLAAQMLGGRVMFMRVNRGQWVAVIELDRALDAR
                     LRGEPGRPAALAP"
BASE COUNT          637 a          858 c          810 g          489 t
ORIGIN      
        1 agaaccccag gcgagagaac gaatacgcgc agcattgcta aaatgttgct cgcgattacc
       61 aacccatctg gcccatccgc acaaggatgc gcgcgctgta gttattcatg cgctcgggcc
      121 gtcgcttcag gggagccggc agaatcgcgg cgagccgtgc cgcctgctcc cggccgatat
      181 tccgggccgc gtttccgtcg tagtagcgac acgccgactc tgcaccataa atgccagggc
      241 cccattcgac gatgttgagg tatatctcca gaatccgccg cttgccgagg acaaattcgg
      301 ctaccggcac cagagtgaac tccgcgccct tgcggagcat ggatcggccc gttccgaaga
      361 ataggttctt tatgagttgc tgcgtaatcg tcgaagctcc acgagtgcgc ccgccttcca
      421 aatcgtcttc ggcagcgatc tggacctggt gccaatcgaa cccatggtgc tggtagaagc
      481 gcgcgtcctc cgcagcgact acagcgtgct ggagattggg cgagatttgg ctgagcggag
      541 taaatttata gcgtttgtga tacggcgtgg ggtgagtcca cgcctgcaag cggcgctgga
      601 tgtgcaccgc ggttgtcggt gggtcgatcc agcgggcggc cagaagaatc agcgcggcta
      661 gcgaccaaag aagcaccacg ccgatggcaa gccatcgaac aaagaatcgg agagaatccc
      721 tgcgcagcga tgtcttggag tgcgtgtctg gcttttcagt cacattttag aataatagcc
      781 tgggctggct tgccgcctat ggctgaccct tcgcgccgcc attcatgagc aagccaatgg
      841 gaaaattagc gtctgcccgc agcgtatttt ctccaggcga tcccgaccaa gccagtgccc
      901 agcaacagaa cgccaccggg ctccggggcg ggcgattgat agcggaaatc atacgtttca
      961 gtgacgccgt tcaacgtcag cgacaaggtg ccttgtacca tcttgtagct gatcggcagg
     1021 agaaagccgt agcccactgc acacgtcgcg gaacactgaa tcggaatcgg accgagtgtg
     1081 aactgcgcgc ccagaagact gatcgtggat gagaacacca cggacccgat cgggtccatc
     1141 gaagtgtcga acaccaatcc tcgctgcccg ccgtgaatgg ccactggaac aagaagcggg
     1201 tccaagcccg acccaagagg ttccaagcga atggggtttt cttgcagcgc gaaggtatgc
     1261 aaggagatcg aatccgcgca ggcaggcagc gccgcgaaca aaaggatgat cagaacagca
     1321 aggcatttca tgaccggacc atcctggtta gcgttcgcgg caacagaact ccgcgatgat
     1381 gtctcctatt gtaagcctca tgcaatacgg tttcacagca cccaaaaaag aacgaacgcc
     1441 tagtgcagca gaaattgctc cggctttcgg ttgacatggc ggccataaac accgctaaca
     1501 ctctttgaaa tgtcgggcgg cccccggatg aaatcgccgc ttcggttcag tgccgcggtt
     1561 cggtgtcctt tgattcacgg cgccagtgcc gctggccgcc ctggctcgcc acgcagccgg
     1621 gcatcgagcg ctcggtccag ttcaatcacg gcgacccact ggccgcgatt gactctcatg
     1681 aacatgacgc gtccgcccag catttgggcg gcgaggacgg gcgggacgtc gcgatgaaat
     1741 ccaaaaaatc gtgtcttgat gctttcccac gcgcgccagc gatcgaaaag cgtcggatgg
     1801 ggttcgtatt tcgtcgaaaa caccagcgcg acatcgaagc gcgaccgcgc ctgcgctgcc
     1861 cccagaattt cgtcggcgct gaagtcctcc acgcgtagta cctgcagcgg ctgggcaacg
     1921 tagcccaggt acggctggct gatctccgcc gatgcgggcc acgccgtcag cacgcgcgct
     1981 ttcgggtaat ggctctccag ataagactca gcctcccgat gcataacgat gtaatcgcgg
     2041 tacgcgagat tgtcttcggg cgcgaatccg tacggcggat tgaagagcag agccaacacg
     2101 aacgcgacgc atgcgagacc cacgagccat cgccattgcc gaatgcgccg ccacagcgtc
     2161 gacactgcaa ccagaatgac cagcggcacg accgtcagca tgtagcgcgc cagcaccgca
     2221 ccgcctacca ccgccatggc cgccacatag gtgagtatca acgcggcgaa caagcactgc
     2281 accggaatcg cgatgcgcgg ccgctctcga tcaccgtcgc gcagcgcagg gcgcgtcatg
     2341 gcccacatca tcaggagcat gagcacccac aggtgcatat aaccgaacga ttgccacagc
     2401 cgcattccca gcgccagtac gatccgcacg ccgctcatgg tggcttgcac gttgtagcgg
     2461 aaaaattccg gattgccgaa aacgtaccca gtgcgcgcgt agtggtagcc ataccaggaa
     2521 cagagcacca gaatcggaac cgataatgac gcgaactgga tgcgaggaaa gggtttccta
     2581 tcagggcatc gctctggcga cggcatcgac atcgtacttg caagcccctt caggggcccg
     2641 aacggcagca ggtatgtaac catctcccac gccaacaaac ccaaaggcgc cagcacggcg
     2701 gtctctttcg cgagcacagc gagcgaaaac cacataaccg cagcgcgcca gcgttgcccc
     2761 agataggctt cgagtcccca taaggtcagt ccgg
//
LOCUS       DASTPM010000396         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1109741, whole genome shotgun sequence.
ACCESSION   DASTPM010000396 DASTPM010000000
VERSION     DASTPM010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109741"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..285)
                     /locus_tag="VFR42_06920"
     tRNA            complement(209..285)
                     /locus_tag="VFR42_06920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(249..251),aa:Arg,seq:ccg)
     gene            complement(354..707)
                     /locus_tag="VFR42_06925"
     CDS             complement(354..707)
                     /locus_tag="VFR42_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368922.1"
                     /translation="MQETRRSLLRLFPAAAALAAVVALARGQDPFPPPRQQRPGRSGD
                     DDSNLPEKPSTKAVLEENQKDIKKNVEKLFDLASQLKEQVEKTDSTTTLSLALVKKAE
                     EIEKLARQIKERAKG"
     gene            811..1686
                     /locus_tag="VFR42_06930"
     CDS             811..1686
                     /locus_tag="VFR42_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU0368923.1"
                     /translation="MSSLAPYLTENSFSTLLHLLVILVITLLLNRFLRMLSELLIRPA
                     SGQSRAEQAREQQTRTLASVAYGAASKVVWLAAILTALQQIGISPLPALVLAGVAGLA
                     VGLGAQSLVRDVISGCYIVLEDQYVIGDTVQIGGTLGRVEQLSLRRTVLRDSRGAQVT
                     IANGEIRSVANLSRDYAQAVVDVALAPEVALEKSLAALESAAAELRSDAAWSQALVDG
                     PRVLGVQSYDRNASVVRVQVRTLPMRQEEISRELRRRIQLAFQKEDIPLAGALRVEMT
                     NSQTKDAMPDSPQSN"
     gene            1704..2150
                     /locus_tag="VFR42_06935"
     CDS             1704..2150
                     /locus_tag="VFR42_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368924.1"
                     /translation="MATISYDEVNLMLRLYDLRREPKLRQARSWYLDYFHLDSPDEMM
                     EKYKAGSDEYNYIRMVLSYWEMVASIVNRGLINDELFFESNGEMWVVWDRIRAIVPTW
                     RAAYKNPLVFCNLEEACKRLEGWREKRAPGSNAAMRQMMEQARTKL"
BASE COUNT          548 a          673 c          837 g          530 t
ORIGIN      
        1 ttcagcacgg tctttgccgg acaagccgtc ggcatcagag aagtgcacga cgatatctgg
       61 ctggtgagct ttatggatta tgatctgggg tacttcgatc tggagactcg ggtgctcgaa
      121 ccgctcgaaa atccgttcgg cccaaaagtg ttacccatgt cttaggtacg ttgtgttact
      181 catgtctccg ggccggaccg tctaggaatg gcgcgctcgg ggcgattcga acgcccgacc
      241 ttgtgcttcg gaggcacacg ctctatccag ctgagctacg agcgcgcaaa agaacaactc
      301 tctttattgt aacgcgaaac gcgcgggttt gcgtgaaagg gccggtacgt gagtcagccc
      361 ttggcgcgct ctttgatttg ccgcgccagc ttttcgattt cttcggcctt cttaaccaga
      421 gcgagggaga gcgtggtggt cgagtcggtt ttttcgactt gttcttttag ctgggaagcc
      481 aagtcgaaca gtttctccac attctttttg atgtctttct gattttcctc gagcacggct
      541 ttggtggaag gcttttcggg cagattggag tcatcatctc ccgaccgtcc tgggcgctgt
      601 tgcctggggg gaggaaatgg atcctggccg cgagcgagcg caacaacggc ggccagcgcc
      661 gcggcggcgg gaaagaggcg caaaagcgaa cgtcgagttt cctgcatgat tcatgtcctc
      721 ggcagaaaat gggacgctgc agcgtgacca aggtggaaag cacgcggctg gcccggctac
      781 tcaatgttat actcccccgg cgaatcgtct atgtccagtc tggctcctta tctgactgaa
      841 aacagttttt cgacactgct gcatctgctg gtgattcttg tcatcacact gctgctgaac
      901 cggtttttgc gcatgctcag cgagttgctg atccggcccg ccagcggcca atcacgcgcc
      961 gaacaagcgc gcgagcagca gacccggacg ctggcaagcg tggcctatgg cgcggccagc
     1021 aaagtggtgt ggctggcggc gattctgaca gcgctgcagc aaattgggat cagtccgctg
     1081 cccgcgctcg tgttggctgg agtggccggg cttgctgtgg gcctgggagc gcagagcctg
     1141 gtccgtgacg ttatcagcgg gtgctacatc gtattagagg accaatacgt catcggagac
     1201 acggtacaaa tcggcggaac gctgggacgc gtcgagcaat tgtcgttgcg gcgcaccgtg
     1261 ctgcgggatt cgcgcggagc gcaagtgacg atcgccaacg gggaaatccg cagcgttgcg
     1321 aacctgagcc gcgactacgc gcaggctgtg gtggatgtgg cgctagctcc ggaagtggcg
     1381 ctggaaaagt cgctggcggc actagaatcg gcggcggccg agctacgcag cgacgcggcg
     1441 tggtcgcagg cgctggtaga cggtccgcgc gtgttgggag tgcaatcgta tgaccgcaac
     1501 gccagcgtgg tgcgagttca ggtgaggaca ctgccgatgc ggcaagaaga aatttcgcgg
     1561 gaattgcggc ggcgtatcca actggcattt cagaaagagg acattccgct ggccggagca
     1621 ctgcgagtgg aaatgaccaa ttcgcagaca aaagatgcga tgcctgattc gccgcagtct
     1681 aactgattca ggagctgccc ctcatggcca ccataagcta cgacgaggta aatctgatgt
     1741 tgcggctcta cgacctgcgg cgcgagccaa agctgaggca ggcgcgcagc tggtatctcg
     1801 attattttca cctggattcg ccggacgaaa tgatggagaa gtacaaagca ggaagcgatg
     1861 aatacaacta catcagaatg gtgctgagtt attgggagat ggtggcgagc attgtgaatc
     1921 gcggactgat caacgacgag ctcttctttg aaagcaacgg cgaaatgtgg gtggtgtggg
     1981 accgcattcg ggctatcgtg ccgacgtggc gcgcggcgta taagaacccg ctggtgtttt
     2041 gcaatctgga agaggcctgc aagcggctgg aagggtggcg ggaaaagcgc gcgcccggat
     2101 cgaacgcggc gatgagacag atgatggagc aggcgcggac gaaactttga gcgcagacca
     2161 cggggagagt gacgggttaa ctggcgtggt gggggtttcc tctttgcgga ccggcgcgac
     2221 ggtgtcctgc aggccagagg gtaggacgag aaccgaccgg ttgcgaccgg attccttagc
     2281 gcgataaagg gccacatccg cttcgtgaat caacttcgac actcaagcaa gccactccaa
     2341 tcgtcagtgc cggccagccc agactcatgg tgacatgcat attccggaga attggccagg
     2401 ctaggtactc gcccgcttcc ggttgagcgg ccataacgcc ggattcggca gcgagaggca
     2461 tgccagcagt aaaccaattg taggaattct ggtcacacct gcgtcccagc ggacaccacg
     2521 ccgcacaaag gacgccaatt cttacgcggg tgttagaacg taagaagttg aaaagagtgg
     2581 actttagc
//
LOCUS       DASTPM010000397         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_648136, whole genome shotgun sequence.
ACCESSION   DASTPM010000397 DASTPM010000000
VERSION     DASTPM010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648136"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFR42_06940"
     CDS             complement(<1..703)
                     /locus_tag="VFR42_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0368925.1"
                     /translation="MKFPWSRNRQDRLEEELQAHVQMAAQDRIDRGESPTQATQFARR
                     EFGNVGLVQQVTRDQWGWRWLEEMLQDLRYGARMLRKNPGFTLIAVLTFALGIGATTA
                     IFSVVNGVLLNPLPYPHPEQLVTLHQNKANFEFGSISYPNFRDWQKDNHTFSSMAISR
                     GYAFSLTGVGEAEQVRARFISSDFFSIVGVNPVLGRTFAPGEDEIGAAPIVLITAGFW
                     KRKFGSSPDVLGKTLT"
     gene            complement(732..>2329)
                     /locus_tag="VFR42_06945"
     CDS             complement(732..>2329)
                     /locus_tag="VFR42_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0368926.1"
                     /translation="PLRILRQLLTESLLLAMVGGALGILFASWCVHSISAFIASSTDG
                     PATFAPGVDVRVMLFTAAISILTAILFGLAPALRGLRVELAPTLKEGTRSSLQAGGTA
                     GKKWLTAGNALVVVQVALSIVVLAGAGLLVRTLQNLKTANPGFDTRNILTFSLDPTLL
                     GYKPAATASLYHQLQGRFAAMPGIRSVSYSWRPLLAGSLWTTDFPLPGKPKEDKVNAD
                     MLPVGAQFFHTMGIPMLSGREFNGADFQRAENAAATLAAQQEEFAARLKNPAAPQTKS
                     SASSNVAAVPAIVNDAFVRRYLTGDPVGQQFGAEQGNPETREPQRPGWEIVGVVANAK
                     YNDLRRDTQPTVYVPHSGGMVTFVLRGFGDVAVSAVQIRSIVSQIDTNLPVFSIKTEQ
                     EHIDRKIFKERLIARLSGFFGVLALLLACVGLYGLISYEVARRTREIGIRTALGADKR
                     DVLRLVLTQGMRLVMAGSLIGVVLALALMRYAASLLYGVRATDPATFVAVTVLLAGVT
                     LAACYVPARRATAVDPVVALRYE"
BASE COUNT          515 a          691 c          692 g          431 t
ORIGIN      
        1 aagtcagagt cttgccaagg acatccggtg aggagccgaa tttgcgcttc cagaagccgg
       61 ccgtgatcag cacgatgggc gcagcgccga tttcgtcctc accgggagca aacgtgcggc
      121 cgagaacggg attcacgcca acgatcgaaa agaaatcgga agagataaag cgcgcgcgga
      181 cctgctccgc ttcgccaacg ccggtcaggc tgaaggcata accccgcgag atggccatgg
      241 aagaaaaagt gtgattgtcc ttctgccagt cgcgaaaatt gggataggaa atcgagccaa
      301 actcgaagtt ggccttgttc tggtgcagcg tgacgagctg ttctggatgc gggtacggca
      361 gcggattgag cagcacgcca tttacgacgg agaaaatcgc ggtagtggca ccgatgccca
      421 gcgcgaacgt gagcaccgca atgagcgtga atccaggatt ttttcgcagc atgcgcgcgc
      481 cgtatcgcaa gtcttgcagc atctcttcga gccagcgcca gccccactga tcgcgggtaa
      541 cttgctggac caagccgaca ttgccgaatt cacggcgcgc aaattgagtt gcttgcgtcg
      601 gtgattcgcc gcggtcgatg cgatcctgcg cggccatctg cacatgcgcc tggagttcct
      661 cctccaagcg gtcctgccga ttgcgcgacc acgggaattt cactttcaac tccttcgaga
      721 gatcgaagcg gttattcgta acgcaaagcg accacgggat cgacagcggt agcgcgccgc
      781 gctggcacgt agcaggccgc caaagttacg ccggccagca aaactgtgac cgccacgaat
      841 gtcgcgggat cggtcgcccg cacgccgtag agcaagcttg cggcgtaacg catcaaggcg
      901 agcgcgagca ccaccccgat caacgaccct gccatcacca ggcgcatccc ctgggtcaag
      961 accaggcgca ggacatcgcg cttgtctgct cccagggccg tgcggattcc aatttcacgc
     1021 gtgcgccgcg cgacttcata ggaaatcaaa ccgtatagcc caacgcaggc cagtaacaat
     1081 gcaaggacgc cgaaaaatcc ggaaaggcgc gcgatcaaac gttccttgaa aattttcctg
     1141 tcgatatgtt cctgctcggt tttgatgcta aagactggta ggttcgtgtc gatctgggac
     1201 acgatagaac gaatctgcac ggcagacacg gcaacgtctc cgaagccgcg caagacgaat
     1261 gtcaccattc ctccgctgtg cggcacgtaa acggtcggct gagtgtcgcg tcgcaggtcg
     1321 ttgtacttgg cattagccac gacaccaaca atttcccaac cggggcgctg cggctcacga
     1381 gtctctggat ttccttgctc tgccccaaac tgctgcccga ctggatcgcc ggtcaagtac
     1441 cgacgcacaa atgcgtcgtt aacgatcgca ggaactgcag caacgttgct cgaagcggac
     1501 gacttagtct gcggcgccgc tgggttcttc agccgggcgg cgaactcctc ttgctgtgcc
     1561 gccagtgtgg cggctgcatt ttccgcccgc tgaaagtccg cgccgttaaa ttcccgaccg
     1621 gatagcatgg gaatgcccat ggtgtggaag aattgcgcac cgaccgggag catgtccgcg
     1681 ttaaccttgt cttccttggg cttgcccggc aagggaaagt cggtggtcca caacgatccg
     1741 gctagcagag gtcgccacga ataactgacc gagcggatac ccggcatggc ggcgaaccgt
     1801 ccttgcagtt gatgatagag gctggcggta gctgcaggtt tgtagcccag caaagtagga
     1861 tcaaggctga aggtcaaaat atttcgggta tcgaatccgg gattcgccgt tttcagattt
     1921 tgcagcgtgc gcactagcaa tcccgcgccg gccagcacga cgatgctgag ggccacctga
     1981 accaccacca gtgcattgcc cgcggtgagc cacttcttcc ctgccgttcc gccggcctgc
     2041 agactggacc tggtgccttc tttcagagtc ggcgccaatt ccacgcgaag cccgcgcagc
     2101 gctggcgcga gtccgaacag gattgcggtg agtatggaaa tcgccgcggt gaacagcatc
     2161 acgcgcacgt cgacgccggg agcaaacgtt gcgggaccgt cagtactgga ggcgataaaa
     2221 gcgctgatgg agtggacaca ccaggaggca aataaaatgc ccagcgctcc acctaccatt
     2281 gcgagcaaca agctttcggt cagcagctgg cgcaggattc gcagcggcg
//
LOCUS       DASTPM010000398         6313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_734876, whole genome shotgun sequence.
ACCESSION   DASTPM010000398 DASTPM010000000
VERSION     DASTPM010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734876"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1117
                     /gene="waaF"
                     /locus_tag="VFR42_06950"
     CDS             77..1117
                     /gene="waaF"
                     /locus_tag="VFR42_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEU0368927.1"
                     /translation="MRLLIRATNWVGDAIMALPALQALRKRFVDAHIAILALPYVAEV
                     YQGQGIADELIVYDRKGEHAGIRRRERLVATLESKQFDTALLLQNAFEAAWIVWRAGI
                     PLRIGYNRDGRGLLLTKAIPVPKAGEIPAHEKFYYLELVRRVGWLESLPDVPEIALRV
                     KAEQLEEAEGKLRVLGARVGVTRVAVGAGASYGSAKCWPPERFAQALNQLRSERDADI
                     VLFGTPAETNVSAAIQAGLSQPALDLTGKTQIAELSALLSRCSVFLGNDSGAMHVAAA
                     VGLPVVAVFGPTDPFGTAPVTPRCTIVQEMPYCSPCFLRRCPTDHRCMTAVTPEMVSA
                     PLLRTLAEVPSA"
     gene            1128..1682
                     /locus_tag="VFR42_06955"
     CDS             1128..1682
                     /locus_tag="VFR42_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0368928.1"
                     /translation="MRPAVFLDRDGTIAEEVGYLNHISRFRMLSGVAAAICKLNEAQV
                     PVVVVTNQSGVGRGYFPESLVGEVHERMISELAKESAHLDGVYYCPHVNADECACRKP
                     KTGMLQRAAADLRLDLKKSFVVGDRGGDVELAYRTGSRSILVRTGFGEGELAWHAAKW
                     KHQPDFVAKDLSEAADWILMELRR"
     gene            1679..2737
                     /locus_tag="VFR42_06960"
     CDS             1679..2737
                     /locus_tag="VFR42_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0368929.1"
                     /translation="MRATKKSLPNGSADLAALAEVVERFAGKRIVLFGDFVADEFQFG
                     DISRVSREAPVLILRHRETRVVPGGGANAANNLAALGAKVSPVTVVGDDAVGAALVQQ
                     FHHSGIDTSGIVRAKNWNTPTKTRFLAGWAHTTAQQVLRVDREPGCAPPDATLARVAA
                     KLQSKLTKADALAVSDYGFGAASSASVGKAVRAMKRKIPVALDARYQLDAYVGSGITA
                     ATPNEAELEALHHTAIGRNVAELEKRGLVTLKKMGLQSLLVTRGKDGMALFEKAGKKV
                     SHIPVHGSDQAVDVTGAGDTVLAAYTLALACGADAFEAAHIANIAGGLVVMKRGTAVV
                     TRGELLAAIRGEVSGSPA"
     gene            2745..3230
                     /locus_tag="VFR42_06965"
     CDS             2745..3230
                     /locus_tag="VFR42_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU0368930.1"
                     /translation="MAREKIVEREELPEIAKDLRQQAKKIAFANGCFDILHVGHVRYL
                     AGARAEADALVVGVNADSSVCSLKGPGRPVLDENARALLVAALRAVDYVIIFPEPNVE
                     SLLEELRPDVHAKGTDYTAETVPERAVAARLGIRVAIVGDPKNHSTRELLQTVRQAPN
                     G"
     gene            3223..4224
                     /locus_tag="VFR42_06970"
     CDS             3223..4224
                     /locus_tag="VFR42_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0368931.1"
                     /translation="MAEPRFLVVRLGSLGDIVHAFPAVSGLRKSFPNAEIVWLTHPKW
                     EALIRAGNLADETWTVDTRDWASLRGCLARLHGHHFEAAIDYQGLWKSALIPFLAGVH
                     RRIGFSSETVREAGVPLLYTDRVRVNAASHVADQNGELSLRAGATQRTAEVEIRVSPE
                     GSASVKETLRKQGITEYVLLSPGGGWRSKCWPAERFGKLALRILEELQLRCVINAGPG
                     EEDLVAAVIKAAGSAQPPAYSGNMGQLMALLKDAAAVVAGDSGPLHLADALGTPVVAI
                     FGPTDPSRNGPYRHSGVVLRWEGAETTYKRGTEPDQSLLHVSVDEVFSALRGLREAA"
     gene            4221..4778
                     /locus_tag="VFR42_06975"
     CDS             4221..4778
                     /locus_tag="VFR42_06975"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0368932.1"
                     /translation="MSDAAALFARWRVRLGYLLAIFVLWFARPTPRSVFYGGLVGLLG
                     LWIRAYAAGYLHKQSVLTVTGPYAHTRNPLYLGSSVLALGAAVATRSWISALILSAYF
                     AGVYAVVMRREERELYAQHGQSFKAYADAVPLFLPRLTPAKASGASPGSFSFAQYKKN
                     HEYEAAVGFLLLLFVLLVIWRLRPV"
     gene            complement(5123..>6313)
                     /gene="rlmD"
                     /locus_tag="VFR42_06980"
     CDS             complement(5123..>6313)
                     /gene="rlmD"
                     /locus_tag="VFR42_06980"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU0368933.1"
                     /translation="KAETVSRRKKLIWAKPLEIAKPNAARVGPFCSHFRTCGGCHYQH
                     ATYDAQVQMKLDILRETLSRMGGVNWTSEIKAHTAEPRHYRNRAQWAVRHANPRALGY
                     YLPESNTVMAVDRCEVLSPALADAFSSLQEITRSGSLPPEVLEIEAFADSADAKIALN
                     VAFKQFAAPVTQLTKLFRDAVPSLESLLLLDQKTNRFELSGPGYLVHQAGGYDFRVSH
                     LSFFQVNRFLIEDLLRTVVAGAQGDFALDLYAGVGFFTLALAKQFRRVVSVDANLSAT
                     RNLRENAEKAGVAVVSQNEHTEEFLRKTSEKPDLVVLDPPRSGLGQAGAQKLTDLGPN
                     EIVYLSCDPSTLARDLSVLLQTSRKPAELPEPAHKYEITSVDFFDLFPQTFHIETLVR
                     LRRM"
BASE COUNT         1256 a         1667 c         2085 g         1305 t
ORIGIN      
        1 acctgatctt cagttcggag tgcgaatttg taccggcgct ggaccgaatt ttgaaggaac
       61 gccgaggaaa aagttcgtga gactgctgat tcgcgcgacg aattgggtgg gcgacgcgat
      121 tatggcgttg ccggcgctgc aagcgctgcg caaacggttt gtggacgccc acattgcgat
      181 tttggccctg ccgtacgtgg cggaggttta ccaagggcag ggcattgcag acgaattgat
      241 tgtgtacgat cgcaaggggg agcacgcagg gattcgaagg cgcgagcggc ttgtggcgac
      301 tcttgagtcg aaacagttcg atacggcgct gcttttgcag aatgctttcg aggcagcatg
      361 gatcgtgtgg cgcgcgggga tcccgctgag gatcggctac aaccgcgatg ggcgaggact
      421 tcttttaacg aaggcgattc cggtgcccaa ggccggagaa attcccgcgc atgagaaatt
      481 ttattacctg gagctggtgc ggcgagtggg ctggctggag agtctgcctg acgtgccgga
      541 gattgcgctg cgtgtgaaag cagagcagtt ggaagaagcc gaaggaaagc tgcgcgtgct
      601 aggcgcgagg gtgggagtca cgcgcgtcgc ggttggcgca ggcgcctcct atggttctgc
      661 aaagtgctgg ccaccggagc gttttgcgca agctctgaat cagttgcgaa gtgaacggga
      721 cgccgacatc gtgttgttcg ggacgccggc cgaaaccaat gtttcggccg cgattcaagc
      781 cgggctgtcc cagcccgctt tggacttgac cggcaagaca caaatcgcgg agttgtcggc
      841 gctgctatcg cgctgcagcg tgtttttggg caatgattcg ggagcgatgc acgtggcggc
      901 agcggtgggc ttgccggtcg tcgcggtgtt cggacctacg gatccctttg gcactgcgcc
      961 ggttacgcca cgatgcacga tcgttcagga gatgccgtac tgcagtccgt gctttttgcg
     1021 acgatgtccg accgatcatc gctgcatgac cgccgtgacg cccgaaatgg taagcgctcc
     1081 attattgagg acattggcgg aggtgccgtc tgcatgagac gcatccattg cggccggcgg
     1141 tttttctgga ccgcgacggc accattgcgg aagaagttgg ctacttgaat cacattagcc
     1201 ggttccgtat gttgagcggc gtggctgcgg cgatttgcaa gttgaacgag gcacaagtgc
     1261 cggttgttgt ggtgaccaat cagtcgggcg tggggcgcgg atattttccc gagtcgctgg
     1321 tgggcgaagt tcacgaacga atgatttctg aattggcgaa ggaaagcgcg cacctggacg
     1381 gcgtgtacta ttgcccgcat gtcaacgccg atgagtgcgc ttgtcgcaag ccgaaaacgg
     1441 gcatgctgca gcgggcggcg gccgacctgc gattggactt gaagaagtca ttcgtagtgg
     1501 gcgaccgcgg cggcgatgtg gaactggcgt atcgcaccgg cagccggagc attctggtgc
     1561 gaacaggatt tggcgaaggc gaattggcgt ggcacgcggc taaatggaag catcagccgg
     1621 attttgtggc gaaggacctt tcagaagcgg cggactggat cctgatggaa ctgcgacgat
     1681 gagagcgacg aagaaaagtc tgccgaatgg ttccgccgat ctggcggcat tggccgaggt
     1741 cgtggaacga tttgcaggca agcgaatcgt cctcttcggc gatttcgtgg ccgacgaatt
     1801 tcagtttggt gatatttcgc gggtttcgcg cgaggcgcca gtcttaattt tgcggcaccg
     1861 cgagacccgg gtggtaccgg gcggtggtgc caacgcggcc aacaaccttg cggcgctggg
     1921 cgcaaaggtt tcgcccgtta ccgttgtggg agacgatgca gtcggcgctg cgctggtgca
     1981 acagtttcac cacagtggga tcgacaccag cggaatcgtt agggccaaga actggaacac
     2041 tcctacaaaa acccgtttcc ttgcgggttg ggcgcatact acggcgcaac aggttctgcg
     2101 ggtcgatcgc gagccgggct gcgcgccgcc ggatgcaacg ctggccagag ttgccgcgaa
     2161 gctgcaaagc aagctgacaa aggcggatgc gttggcggtg tcggattatg gatttggagc
     2221 ggcctcgtct gcttcggttg gcaaggcagt tcgcgcaatg aagcggaaga ttcccgtcgc
     2281 gcttgatgca cgctatcaac tggacgcgta cgtgggaagt ggaatcaccg cggcgactcc
     2341 caacgaggcg gaactcgagg cactgcatca caccgcgatt gggcgaaacg tagcggagtt
     2401 agagaagcgc ggcctcgtca ccctgaaaaa gatgggtttg cagtcgttgc tggtgacgcg
     2461 tggaaaagac ggaatggcgc tgttcgaaaa agcagggaaa aaggtttcgc atattcccgt
     2521 gcatggtagc gatcaggcag tggacgtgac gggggccgga gacaccgtgt tggcggcata
     2581 cacgctggcg ctggcttgcg gcgcggacgc tttcgaggca gcacacattg ccaatattgc
     2641 cggcggcctg gtggtgatga agcgcggaac tgcggtggtc accagagggg agctgctggc
     2701 ggctatccgc ggagaagttt ccggcagccc ggcgtgagat gcgagtggcc cgcgaaaaaa
     2761 tcgtagaacg agaagagctg ccggaaatcg ccaaggatct gcggcagcaa gcgaaaaaaa
     2821 ttgcatttgc taacggctgt tttgacattt tgcatgtcgg gcatgttcgc tatctggcgg
     2881 gcgcacgcgc cgaggctgac gcattggtgg ttggagtgaa tgcggattcc agcgtctgct
     2941 cgctcaaagg gccgggtaga cctgtgctcg acgaaaacgc gcgtgcattg ctggtcgcgg
     3001 cgctgcgcgc tgtggactac gtgattattt ttcccgaacc gaatgtggaa tcgctgctcg
     3061 aggagttgcg ccccgacgtg cacgcgaaag gaaccgacta cacggcggaa accgtgccgg
     3121 aaagggcggt agcggcgcgg ctgggcatcc gcgtggcgat tgtcggcgat cccaagaatc
     3181 attcgacgcg cgagctgttg cagactgtgc gccaggcgcc aaatggctga accgcgattc
     3241 ctcgtcgtgc gtctcggctc gctgggcgac atcgtgcatg cgttcccggc ggtgagcgga
     3301 ctgcgtaaat cgtttccgaa tgcggagatt gtctggttga cacatcccaa gtgggaggcg
     3361 ttgatccgcg ccggcaatct tgccgacgaa acatggacgg tggatacgcg cgattgggcg
     3421 tcgctgcgag gctgcctggc gcgccttcac gggcatcatt ttgaggcggc gatcgactac
     3481 cagggattgt ggaagtcggc tttaattcca tttcttgcgg gagtgcaccg acgaattggc
     3541 ttttcttcgg agacggtgcg cgaggcggga gttccgctgc tgtataccga tcgcgttcgt
     3601 gtgaatgcgg cgtcgcatgt ggctgaccag aacggcgagc tttcgctgcg cgcgggtgcg
     3661 acgcaacgta cggcggaggt ggagatacgg gtttcgcctg aaggcagcgc gtcagtaaag
     3721 gaaacactgc ggaaacaagg gatcactgaa tatgtcttgc tgagtcccgg cggaggctgg
     3781 cggtcgaaat gctggcccgc cgaacgtttt ggaaagctgg ccttacgcat tctggaggaa
     3841 ttgcaattgc gttgcgtgat caatgcaggc ccgggcgaag aagacctggt cgcggcggtg
     3901 atcaaagcgg cgggaagcgc gcagccgccg gcgtacagcg gaaatatggg gcaactgatg
     3961 gcgctgctga aagatgcggc cgcggtggtt gcgggggaca gcgggccgct gcacttggcg
     4021 gatgcgctgg gtacgccggt cgtggcaatt tttggaccaa ccgatccgtc gcgcaacggc
     4081 ccgtatcgcc acagtggcgt ggtattgcgc tgggaaggtg cggaaacgac gtataagcgc
     4141 ggaaccgaac cagaccagtc cttgctgcac gtcagtgtcg acgaagtgtt cagcgcgctg
     4201 cgcgggctgc gtgaagcggc atgagcgacg cggcagcttt gtttgcgcgc tggcgggttc
     4261 gcctcggcta tctgctggcg atttttgtgt tgtggttcgc gcggccaact ccccgaagcg
     4321 tattctacgg cgggctggtc ggcctgctgg gcttgtggat ccgggcctat gccgcgggct
     4381 accttcacaa acaatccgta ctcacggtca ccgggcctta cgcacacacg cgcaatccgc
     4441 tctacctggg tagctcggtt ctggcgttgg gagcggcggt ggccacgcgt tcttggattt
     4501 cagcgctcat tttgtcggcg tattttgccg gcgtctacgc cgtcgtgatg cgccgcgaag
     4561 agcgggaact ttacgcacaa catggccagt cgttcaaagc ttatgcggat gcggtgcctc
     4621 tgttcctccc gcgattgacg ccagcaaaag cttccggcgc gagcccggga agcttttctt
     4681 tcgcgcagta caagaagaat cacgaatacg aagcggcggt tggatttcta ttgctgctct
     4741 tcgtcctttt ggtcatttgg cgcttgcggc ctgtttgaat taaggatggc ggtgatttgc
     4801 ggacacgcca cgaagcaaga cactttgata cttttcccgt ccgtggagat atggatgacg
     4861 ccctccggtc tgtgcgcagc accggattcg ggcttgctgc aacctttgca gaagaaccga
     4921 cgtcgatccg tacttcggcc atgattggta attcaaacac cctttgcagg gagcgggacg
     4981 cccaatcaag tggacagcgc tctgcttcac gccactcccg gtaccgagct gtccccccgt
     5041 gcgaaccgac ctctaagact gcgaatactc acaaccatgc acgcagcaaa gccagtcacg
     5101 atcgcgatgg ccggcagtct cgtcacattc gccgcagccg cacgagcgtc tcgatgtgga
     5161 atgtttgcgg gaacaagtcg aagaaatcga ccgaggtgat ctcgtattta tgagcgggct
     5221 ctggaagctc agccggttta cgcgacgttt gcaagagaac gctgaggtcg cgggccagcg
     5281 tcgaagggtc acaggataaa tacacaatct cattgggccc gagatctgtg agtttttgtg
     5341 cgcctgcctg ccccaatccg gagcgaggcg ggtcgaggac taccaaatcc ggtttctccg
     5401 aagtcttcct aagaaactcc tcggtatgct cattttgcga aaccaccgca acaccggcct
     5461 tctcggcgtt ttcccgcagg tttcgtgtgg cggaaaggtt cgcgtccacg ctgactacgc
     5521 ggcgaaattg tttcgctagc gcgagcgtaa aaaatccaac gcctgcgtag aggtcgagtg
     5581 caaagtcgcc ctgcgctccg gcaaccacgg tgcgcagcag atcctcaatc aaaaaccggt
     5641 tgacctgaaa aaacgaaaga tggctcacac ggaagtcgta tccgccggct tggtggacca
     5701 gataccctgg acccgacaat tcaaagcgat ttgttttctg gtccagcaac agcaggcttt
     5761 ccagggaagg aaccgcgtcg cgaaacagct tagtgagctg ggttacggga gctgcaaatt
     5821 gtttgaacgc cacattgagc gcgattttgg catctgcgga atcggcgaat gcttcgattt
     5881 ccaatacttc gggcggcaaa gagccgctgc gtgtaatctc ctgcaagctg gaaaaggcgt
     5941 cggccagcgc cggcgacaga acttcacagc ggtcgacggc catcaccgta ttgctttccg
     6001 gcaggtaata accaagcgcg cggggattgg cgtggcgcac agcccactgc gcgcggttgc
     6061 gatagtgcct cggctcggcc gtatgcgcct tgatctcgct ggtccagttc acgccgccca
     6121 tgcgtgaaag tgtctcgcgc agaatgtcga gtttcatctg aacttgggcg tcatacgtgg
     6181 catgctggta gtggcatccg ccacaagtcc gaaaatgaga acaaaacggc ccaacacgcg
     6241 ctgcattcgg cttggcgatc tccagtggct tggcccaaat cagtttcttt ctgcgagaaa
     6301 ccgtctcggc ttt
//
LOCUS       DASTPM010000399         3454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1196355, whole genome shotgun sequence.
ACCESSION   DASTPM010000399 DASTPM010000000
VERSION     DASTPM010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196355"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1463
                     /locus_tag="VFR42_06985"
     CDS             105..1463
                     /locus_tag="VFR42_06985"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU0368934.1"
                     /translation="MANGYSTRPRTSGVFSGLLLIVFGALLLLHNYGHLSLRSVFGHW
                     WPLIFIFWGASKLYERTVAQRQGRTYGWITPGEVFLVLGLMTVVGLVIAWDIAKSRIP
                     DFDIDVGDPYSYPIDMPSQPIPGNAHILIRGGRGTISVRSSDEPSLRLSGEKSIRTLS
                     ERDAEKRAPSIGVGVTQSGDSYEIRPTGFDLGDSRISVNINAVVPKKSALTVRNERGD
                     INISDMGTDVTAGTRNGDIEVNDTAGNVSVETNKGDVKVTDTKGDIKVSGRGSDIEAV
                     NATGSLVVDGEFFGSIRADKISKGVRFVSQHTDLTLTQLGGHFEKGSGNLEIADAPGN
                     LTSRTKNASVSLENVTGKIVLDNTNGSVEVRFSSPPKEDVTINNVRASVTLLMPSGSN
                     FEIQADCHSCDINSEFSGGTLNTTKTEKGDSHLEGKYGAGRGPKIILKSSYDSIEIRK
                     TN"
     gene            complement(1483..1686)
                     /locus_tag="VFR42_06990"
     CDS             complement(1483..1686)
                     /locus_tag="VFR42_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368935.1"
                     /translation="MARATRHSAFISTRRLSAFYNVTEKCVLCEMAKLAEENRIRLAG
                     WRNREEFVENSPEGLAIRVDLVE"
     gene            1985..>3454
                     /locus_tag="VFR42_06995"
     CDS             1985..>3454
                     /locus_tag="VFR42_06995"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0368936.1"
                     /translation="MFLPLRHESMQGRRWPIITIALIAINLLAFLGTHWQIDQEDPQR
                     GEVRAHILLLSASHPDLTMTAEVQEMVDAFRRDHPEAWSQAQSPTRDLADAWDARIRL
                     IDDPAALQSEMDSLSSKYAAMTSTSLLEKYAFIPAHPHALSYLTANFLHGGWLHIIGN
                     MWFLWLAGAILEDTWGRVIYPVFYLVAGVAALQIHAWFNPGSMVPTLGASGAVAAAMG
                     AFLVRFPKTKIEVAVILGVRSLINLMMGRGIRINAAAYWLLPAWLLTEVFSGYLVGKS
                     SGVAHWAHVGGFAFGALAALALRHSGLEHRANAAIEAKVSWTADPAIVQATEQMEQGK
                     LDAAVTTLQSFLPGKPDSVEGHRLLQQVYWRKNDIACYRETMAKLIQMQLKSQNLEAA
                     WQDYEDFRNGGGEQLPAGVWLELCRAVEGQGNYERAVSEYDRLATALPSERPALLALL
                     AAGRLSLKNLNRPGEALRFYEAAAKSPVPHLDWESNISKG"
BASE COUNT          746 a         1022 c          967 g          719 t
ORIGIN      
        1 ggacggacat gtcgaaagcg ccgtaccgcc gccggtgaat cccgcgcctc ccgccgagcc
       61 gcccacgtac tccaatccct acgcaggaca agggcagtaa gcgcatggca aacggctatt
      121 caacccgccc gcgtaccagc ggagttttta gcggactgtt gctgattgtc tttggcgcgc
      181 tgttgctgct gcacaactac gggcacctgt ccttgcgcag tgttttcggc cactggtggc
      241 cgctgatctt tattttctgg ggagccagca aactttacga acgaaccgtc gcgcagcggc
      301 aggggcgcac ctatggatgg attactccgg gcgaagtttt tctcgtgctg ggactgatga
      361 ccgtggtcgg tctggtaatc gcgtgggata tcgccaagtc ccgcatcccg gacttcgata
      421 tcgacgtcgg cgacccgtat tcttacccaa ttgacatgcc gtcacagccg attccgggca
      481 atgcccacat tctgattcgc ggcggtcgtg gaaccatctc ggtgcgttcg tccgacgagc
      541 cgagtttgcg gctctcaggc gaaaagtcga ttcgcacact aagcgaaagg gatgcggaga
      601 agcgcgcacc gtcgatcgga gttggcgtca cacagagcgg cgacagctat gaaatccgtc
      661 ctaccggctt cgatctgggc gattcgcgca tatctgtgaa tatcaacgcg gtagtgccga
      721 aaaaatccgc cttgaccgtg cgcaacgaac gcggtgacat caacatttcg gacatgggaa
      781 cagacgtaac agccggcact cgcaacggcg atatcgaagt aaacgacacc gccggcaatg
      841 tcagcgtcga aaccaacaag ggcgacgtca aggttaccga caccaaaggc gacatcaaag
      901 tttccgggcg cggcagcgac atcgaagcag taaacgccac cggcagcctg gtggtcgacg
      961 gagaattttt cggctccatc cgcgcggata agatcagcaa aggcgtgcgc ttcgtatcgc
     1021 agcataccga cttgacgttg acccagctgg gcgggcattt tgagaaaggc tctggcaacc
     1081 tggaaattgc agacgcgccg ggcaacctga cgtcccgcac caaaaacgct tcggtatccc
     1141 tggaaaatgt gaccggcaag atcgtgctcg acaataccaa tggctcggtg gaagtgcgct
     1201 tttccagtcc gcccaaggaa gacgtaacca tcaacaacgt acgtgcttcg gtcacgctgc
     1261 tgatgccttc tggttcaaac ttcgagattc aagccgactg ccactcatgc gacatcaatt
     1321 cggaatttag cggcggaact ctgaatacca cgaagacgga aaaaggcgac tcgcacctgg
     1381 aaggcaagta cggcgcgggc cgcgggccga aaatcatttt gaaatcgagc tacgactcga
     1441 tcgaaatccg caaaaccaat tagcagtttt gcagcaacga aactattcca ccagatcgac
     1501 tctgattgcc agtccttccg gcgagttttc cacaaactct tcccgatttc gccaaccggc
     1561 taggcgaata cgattttctt cggcgagttt ggccatctcg cagagcacac atttttccgt
     1621 cacgttataa aatgcggaaa gtctgcgcgt actgataaat gccgagtggc gcgttgctcg
     1681 cgccaagcga agaatctccc gcctcatcaa ctctgttgaa gtcatttcca aacctcggat
     1741 taatttctta ctttagaact tcttgctccg ggaccgggac tatcttggtt ggactgaggg
     1801 acgaacgaac ccgccgagta taccgtcccg ggcgagattt gcaacccgca ggctgaccgt
     1861 tgggacaact aaccagtcgt gctcacctgt actttggttc cactcctgcg ccgaccgtcc
     1921 ctccgtatgc ttaggaaagc aagtatcacg tccgtacgcg cgaccaatcg gacctggagg
     1981 gaccgtgttc ctgccgcttc gccacgaaag catgcaaggc cgccgctggc cgatcattac
     2041 catcgcgctc attgccatca acctgctggc ttttctcggc acgcattggc aaatcgacca
     2101 agaagatccg caacgcggcg aagtccgcgc acacatcctg ctgctttctg cgtcgcatcc
     2161 cgatttgact atgactgcgg aggtccagga aatggttgat gccttcaggc gcgatcatcc
     2221 ggaggcgtgg agccaagccc aatctcccac gcgtgatttg gccgacgcct gggacgcgcg
     2281 gatacggctg atcgatgacc ccgcggcgct gcagtcggag atggattctc tcagctcgaa
     2341 atacgccgcc atgaccagca cttcacttct ggaaaagtac gcgtttattc cagcgcaccc
     2401 gcatgcgctc tcttatctca cagccaactt tctacacggc ggctggctgc acatcatcgg
     2461 aaacatgtgg tttctttggc tggccggcgc aatcctcgaa gacacctggg gacgggtcat
     2521 ctatcccgtc ttttatctgg ttgcgggtgt tgcggcgctg caaatccacg cctggttcaa
     2581 tccgggcagt atggtcccga cgctgggagc ttcgggagca gtcgctgcgg cgatgggcgc
     2641 atttctggtg cgattcccca agaccaagat cgaagtcgct gtgatcctgg gcgtccgtag
     2701 tctgatcaac ttgatgatgg gccgcggcat tcgcattaac gcggcggctt actggctgct
     2761 gcctgcctgg cttctgacgg aagttttctc cggctatttg gtgggcaagt cgtccggcgt
     2821 agcgcactgg gctcacgtcg gaggatttgc cttcggagcc ctggccgcgc ttgctctacg
     2881 tcactccgga ctcgagcatc gtgccaatgc ggccatcgaa gccaaggttt cctggaccgc
     2941 tgaccccgcc attgtgcagg caaccgagca gatggaacaa ggcaagctcg acgccgctgt
     3001 aactacgctg cagagttttc tgcccggcaa acccgattct gttgaaggcc accggctact
     3061 gcagcaagtt tattggcgca aaaacgacat cgcctgctac agggagacga tggcgaaatt
     3121 gatccagatg cagctgaaat cacagaatct cgaggcagcc tggcaggatt atgaggattt
     3181 cagaaacggc ggcggcgagc aattgcctgc tggcgtgtgg ttggaacttt gtcgcgcagt
     3241 ggaaggccag gggaactacg agcgcgcggt gagcgaatat gaccggctgg cgaccgccct
     3301 tccgtccgag cgtccggcgc tccttgccct gctggccgcg ggccggctct cgcttaagaa
     3361 tttgaatcgc cctggcgagg cccttcgttt ctacgaagcc gccgctaaat caccggtccc
     3421 gcacctcgac tgggaatcca atatcagcaa agga
//
LOCUS       DASTPM010000400         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_475454, whole genome shotgun sequence.
ACCESSION   DASTPM010000400 DASTPM010000000
VERSION     DASTPM010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475454"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1674
                     /locus_tag="VFR42_07000"
     CDS             <1..1674
                     /locus_tag="VFR42_07000"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEU0368937.1"
                     /translation="SELMFLSEAEKSLPWTDYRPSLEDTAHLEDSVTSFRRDRETTSR
                     LEKIFAQLPGTPDLPHYLQSLLKIATAVGGIRDRDSLQWQLLGFLFDVFPAERGAVIY
                     FDKNGGVESTATWDRLSGPEHPVPVDESLLQRVFAGKSALLAPLRGLSGKTEERSGSV
                     LCVPMMTEKVLGAIYLHSSVDRSAGPFDQTHLQVLSAVGSIAALALQNLCHWERISNE
                     NRALRAEVSLEHNIVGASPRMKDVFEFVRRVAPTDATVLIEGESGTGKELVARAIHRN
                     SARAENSFVAINCAAIAENLLESELFGHEQGAFTGAAAQKKGRIEMAEGGTLFLDEVS
                     ELAPELQAKLLRVLQEREFERVGGTRAIPLDIRLVAATNKKLAAAVAAGEFRKDLFYR
                     LNVVTLTMPALRDRAEDIPQLAEHFLAKSSRKSKTRIKKLSDEARVCLIKYDWPGNVR
                     ELENAIESALVLGANDTILPEDLPEAVLEAGPAYPDEHADESGNYLRSVKENKRQTIL
                     QALQQAGGSYLEAAKILGIHPNSLLRLMRNLNVKASMKAGQPPSRDFKK"
     gene            complement(1742..>2374)
                     /locus_tag="VFR42_07005"
     CDS             complement(1742..>2374)
                     /locus_tag="VFR42_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0368938.1"
                     /translation="NEIPGLVEAPLRSLLPIAASIRRRGKGLPGSFATTELNMRNFLL
                     GMVAGALLFVLCGVAYLGLGFAEVRADLAPAAWEMRLMRPAVHASVRRRAPEMSNPVP
                     PTEENLIAGGKLFINGCSGCHGRAGSAEDISSSLFPPIPQFQKEGTSYTEAQIFWITK
                     HGIRRTGMFANGKWNSDQELWTMAAYIKRMRELPPAVQAALAPKKAEAPK"
BASE COUNT          507 a          742 c          664 g          461 t
ORIGIN      
        1 tcggaattga tgttcctcag tgaagcggaa aaatccctgc cgtggaccga ctaccggcct
       61 tccctggaag acaccgccca cctcgaagat tccgtcactt ccttccggcg cgaccgggaa
      121 actaccagcc gtctggaaaa aatattcgcg caactgccgg gcacgccgga cctgccccat
      181 tatctgcagt ctctgttgaa aatcgccacg gccgtcggcg gaattcgcga tcgcgattcg
      241 ttgcaatggc aactgctcgg ttttctcttc gatgtgtttc ccgccgaacg cggcgcggtc
      301 atctatttcg acaagaatgg cggcgtggaa tccaccgcca cctgggaccg cctgtccggg
      361 ccggagcatc ctgtgccggt ggacgaatcg ctgttgcagc gtgtcttcgc cgggaagtcc
      421 gccttgctcg cgcccttgcg cggattgtct ggaaaaaccg aagaacgctc tggctccgtg
      481 ctctgcgttc cgatgatgac ggaaaaagtc ttgggcgcca tttatttgca ctcctcggtc
      541 gaccgctccg ctggaccgtt cgaccagacc catctccagg tgctctccgc agtgggatca
      601 atcgccgcgc tggccttgca gaacctgtgc cactgggaac gaatcagcaa cgaaaatcgc
      661 gcgttgcgcg ccgaggtcag cctcgaacac aacatagtgg gtgcaagccc gcgcatgaag
      721 gacgttttcg aatttgtgcg ccgcgtcgcg ccgaccgatg ccaccgtttt gatcgagggc
      781 gagagcggca ccggcaagga attggtggcg cgcgccatac accgcaacag cgcgcgggcc
      841 gaaaactctt ttgtagccat caattgcgcc gcgattgccg aaaacctgct ggaaagcgaa
      901 ttgttcggcc acgaacaagg agcgttcacc ggcgccgccg cacaaaaaaa aggccgcatt
      961 gaaatggccg aaggcggaac gctgtttctg gatgaagtca gcgaactcgc tccggagctg
     1021 caggcaaagc tcctgcgcgt tttgcaggag cgggaatttg agcgtgtggg cggaacccgg
     1081 gccatacctt tagacatacg cctggttgcg gccaccaaca aaaagttggc cgcggccgtc
     1141 gccgcgggcg aatttcgcaa agaccttttt taccggctga acgttgtgac gctcaccatg
     1201 cccgccctgc gtgatcgcgc cgaagacatc ccgcaacttg cggaacactt tctcgccaag
     1261 tccagtcgta aatccaaaac caggatcaaa aagctctcgg acgaagcccg cgtctgcctc
     1321 atcaaatatg actggccggg caatgtgcgc gaactggaga acgccatcga aagcgctctg
     1381 gtgttaggcg cgaacgatac cattttgccg gaggatctcc cagaagcagt gctagaagcc
     1441 ggcccggcct atccagacga acacgcggat gaatccggaa actatctccg ctcggtgaaa
     1501 gagaataaac gccagacgat tctgcaggcg ttgcaacaag ccggcggcag ctacctggaa
     1561 gcggcaaaaa tcttgggaat ccatccgaat tccctgctgc gactgatgcg caacctgaac
     1621 gtcaaagcat cgatgaaggc cggccagccg ccgtcgcgcg actttaaaaa gtgaatgctg
     1681 cggatgcaga attgcagacc gcgccgagcg cgatcgcatc gaggactgtt attttgggga
     1741 gctatttcgg cgcctccgcc ttcttgggcg ccaacgcggc ctgaaccgcc ggtggcaatt
     1801 cgcgcatccg cttgatgtag gccgccatcg tccacaactc ctggtccgag ttccactttc
     1861 cgttggcaaa cattccggtg cgccggatgc cgtgcttcgt aatccagaaa atttgtgctt
     1921 cggtgtagct ggttccctct ttctggaatt gcggaatcgg cggaaacaac gagctgctga
     1981 tatcttccgc gctgcccgcg cgcccgtggc accccgagca tccgttgatg aacagcttgc
     2041 cgcccgcgat caggttttcc tcggtcggcg ggacgggatt ggacatctcg ggcgctctcc
     2101 gccgcaccga agcatgcacc gccggcctca tcagccgcat ttcccaggcc gcaggtgcca
     2161 gatctgcccg cacttccgca aagcccagcc ccaagtacgc aaccccgcaa agaacgaaaa
     2221 gcaacgctcc agccaccatt cccagcagaa aattcctcat gttcaactcc gtcgttgcaa
     2281 acgatccagg caggcctttc cctcttcgac gaatcgaggc cgcgataggg agcaaacttc
     2341 ttagcggggc ctctaccagt ccgggtattt cgtt
//
LOCUS       DASTPM010000401         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1023085, whole genome shotgun sequence.
ACCESSION   DASTPM010000401 DASTPM010000000
VERSION     DASTPM010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023085"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..994)
                     /locus_tag="VFR42_07010"
     CDS             complement(104..994)
                     /locus_tag="VFR42_07010"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU0368939.1"
                     /translation="MSDYVLSVLLGIIEGLTEFLPVSSTAHLRIAEALLHIDLASGYW
                     KMYTIVIQLGAILCLPVYFRERIANLLKTFPKGERGDRTIFTHPLSLACIAFVVTAIP
                     AFLFTKIIGKHLESLTIIGSSLVIGGIVMWLVDARNAKSEAGGQSAPNSLIDTWRMEA
                     MGIGQAVWIGACQILSAVFPGTSRSMSTIAAGQLAGMSRSAALDFSFFLSIPTMVVAT
                     CYDLLKSLRGKGENAIGVSHIDGHGWIVLAIGFVVSFIVAYAAVAWFLAWVRRRGFAP
                     FAIYRIIVGCAVLAWALRYL"
     gene            complement(1021..1488)
                     /locus_tag="VFR42_07015"
     CDS             complement(1021..1488)
                     /locus_tag="VFR42_07015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0368940.1"
                     /translation="MSLAIRQYEPHDFAAIFRLDQSCFPAGISYSKTMLRYYLAQPGA
                     ECLIAADDGKIVGFLISEENPPLGHIITLDVAQSHRRQGVGSLLLSENEAHMVFRGVR
                     TVLLETGTSNEAGIAFWERHGYRREAVLKNYYPGRLDAFEMRKKLGATPRSKA"
     gene            1835..>2955
                     /gene="rsgA"
                     /locus_tag="VFR42_07020"
     CDS             1835..>2955
                     /gene="rsgA"
                     /locus_tag="VFR42_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU0368941.1"
                     /translation="MYALQELGWCDFFAQQKTQHDDGESSVVPARVAEENRELYRLFS
                     EHGEFLAEVSGKLRHATTSRADLPAVGDWVLAQLRLRENRATIHRVLGRKGKFSRKIA
                     GRKTEEQIVAANVDVVFLVSSLNREFNPRRIERYLTLAWDSGARPVVVLNKADLCDNA
                     EAFRTEAETAAAGAPVVLASATRGDGLAELRALLRGRAEGQEGCEAPALQQQSGLTAA
                     LLGSSGVGKSSLINVLIGEQSAALPGGVPLATQAVRGGDDRGRHTTTTRQLLLVPGGG
                     VLIDTPGMRELQLWDAADGIGRTFGDIQELAARCKFRDCKHQSEPGCAVRAAVEAEAL
                     DAERVESFHKLEREEQFLEAKQDAAVRSQRTKELRKMMKS"
BASE COUNT          639 a          893 c          857 g          566 t
ORIGIN      
        1 aggtaatact cgttttcggt tcctgaaagc ttcctattgc tcgggacatg cgtcgcatgt
       61 cggcgttgcc gttcacactc tcgtgccaac tgctgtaggc tcctcagagg tatctcaatg
      121 cccaagccag aaccgcacag cccacaatga ttcgatagat cgcgaacggg gcaaacccgc
      181 gccgacggac ccaggccaga aaccaggcca ccgccgcata ggccacgatg aaggaaacca
      241 cgaatccgat ggccagaacg atccagccgt gcccgtcgat gtgcgacacg ccaattgcat
      301 tctcgccctt gccgcgcagc gatttcagca ggtcataaca cgtcgccacc accatcgtcg
      361 gaatggaaag aaaaaaagaa aaatccagcg ccgccgagcg cgacatcccg gccagttgtc
      421 ccgccgcaat cgtcgacatg gaccgcgacg ttccggggaa caccgcggac aaaatctggc
      481 aggctccaat ccataccgcc tggcctatgc ccatcgcctc catccgccag gtatcaatca
      541 gcgaattggg agcgctctgt ccccccgcct cagacttcgc gttacgcgcg tccaccagcc
      601 acatcacgat tccgccgatc accagcgaac ttccaatgat cgtcaggctc tccaggtgct
      661 tgccgatgat tttggtgaat aggaaggctg gaatggcagt aaccacaaac gcgatacagg
      721 ccaagctgag cggatgggta aaaatcgtcc ggtcgccgcg ctcgcctttc gggaaagtct
      781 tcagaagatt tgcgatgcgc tcgcgaaaat ataccggcaa acatagaatc gcaccgagct
      841 gaatcacaat cgtgtacatc ttccagtatc cactggcgag atcgatgtgc agcagcgctt
      901 ccgcaatgcg caggtgcgcc gtcgaactga ccggcagaaa ttcagttagc ccctcgatga
      961 tgccgagcag cacagacaac acgtaatcgc tcaatgattc gctccgggat tcgccgcctc
     1021 ttacgctttt gacctggggg ttgcgccaag ctttttgcgc atctcaaaag cgtccaggcg
     1081 gccgggatag tagtttttca gaactgcttc tctacggtag ccgtgccgct cccagaacgc
     1141 aatgcccgct tcattcgacg ttccagtttc cagcagcacg gtgcgcacgc cgcgaaacac
     1201 catgtgtgct tcattctccg agaggagcag tgagcctact ccttgcctgc ggtgcgattg
     1261 cgcaacatcc agcgtgatga tgtgtcccag tggcggattt tcctcgctga tcaggaagcc
     1321 cactatcttt ccatcatcgg cggcaatcaa acattccgct cccggttgcg ccaggtagta
     1381 gcgcagcatc gttttcgaat aggagattcc ggcaggaaag cacgactggt cgagcctgaa
     1441 gatcgcggcg aaatcgtgcg gctcgtactg gcggatggcc aagctcacgc cgctgattct
     1501 atggttctcc gctcttccgg tgaaaactat tttgcgtcgg cgaacgccat tttctgctac
     1561 tctttcccga gccgctccaa gcggcgcggt cactactctt ctggaggcgc ctgtgcgaac
     1621 cgtagccccg atgcgggatg agcgcgtctc cgtgtctctc ctgtaagttc aatttctcca
     1681 aacttgtgag tactagttat tccgagcgaa gcgaggaatc tctcttcgcc gtttccgttc
     1741 gttgcacagg aacgaacgcg ccccgcatgc gcaacggtat gcgggaagga ccgatggttt
     1801 gccgcgcaaa ccatcggtca aaaggagatt cgcagtgtac gcattacaag aattgggttg
     1861 gtgcgacttc ttcgcacagc aaaaaacgca gcatgacgat ggtgaaagca gtgttgttcc
     1921 cgcccgagtt gcggaagaga atcgcgaact ttaccgcctc ttcagcgaac acggagaatt
     1981 cctcgcggag gtgagcggca agttgcggca cgcgacgacg tcgcgtgccg atctgcccgc
     2041 cgtgggagat tgggttctcg cgcagcttcg gctgcgcgag aaccgcgcga ccattcaccg
     2101 tgttctgggg cgcaagggaa aattctcccg taaaatcgcc ggcagaaaga ccgaagaaca
     2161 aattgtcgcg gcgaacgttg acgtggtatt cctcgtcagc tcgctcaacc gcgaatttaa
     2221 tccgcggcgt atcgaacgct acttgacgct ggcgtgggac agcggcgcac ggccggtggt
     2281 ggtcctcaac aaagccgatc tgtgcgacaa cgcggaagcg tttcgcacag aagcggaaac
     2341 cgcagcggcg ggggcgcccg tcgtgctggc cagcgcgacg cgcggcgacg gtcttgccga
     2401 actccgcgcc cttctgcgcg gtcgcgcaga agggcaagaa ggctgcgagg cgccagcgtt
     2461 gcagcagcag agcggcctga cggccgctct gctcggttcc tccggcgtgg gcaagtcttc
     2521 cctgatcaat gtgctgatcg gcgagcagtc agcggcccta cccggtggcg taccgcttgc
     2581 gacgcaagcg gtacgcggag gcgacgaccg agggcggcat accaccacga ctcggcagct
     2641 actgctcgtt cccggcggtg gagtgctgat cgatacgccg ggcatgcgcg aactgcagct
     2701 gtgggatgcg gccgatggaa ttggccggac cttcggcgac atccaggaac ttgcagcgcg
     2761 ctgcaagttc cgtgactgca agcaccagag tgaaccgggc tgcgcggtac gcgcagccgt
     2821 ggaagctgaa gctctcgacg cggaacgcgt cgagagcttt cacaagctgg aaagggaaga
     2881 gcagttcctg gaagcgaagc aggatgccgc ggttcgttca caacgcacca aggagttgcg
     2941 caagatgatg aaatc
//
LOCUS       DASTPM010000402         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_302497, whole genome shotgun sequence.
ACCESSION   DASTPM010000402 DASTPM010000000
VERSION     DASTPM010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302497"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFR42_07025"
     CDS             <1..453
                     /locus_tag="VFR42_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368942.1"
                     /translation="VDVVAGALAELGTEHAFVVHGAGKLDEISLAGPTIMTEVRAGSV
                     RKFEVTPEDFGKKPAPLEMIQGGNLAENAALIRDLLDGVVKTEGDRARCDIVVMNGSA
                     ALVAAGVAGNFREGAELAEKALREGSAARKLEALRQYGDEKGVSPGRV"
     gene            578..3160
                     /locus_tag="VFR42_07030"
     CDS             578..3160
                     /locus_tag="VFR42_07030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0368943.1"
                     /translation="MADVNKHLDRAKKFLEKNRVEDAIEAYLSVLEEAPSHMEATQAL
                     GDLYARREQPDRAAVYYGMFFDLLVDPKDETKALAIYNRFLKHGAQQPPERIARYAFL
                     QQRQNRTDEASEQYGKAAELFTAAAREEDALFCWERIAQLEPDNLPRQIKLADVAEKS
                     GRNLLAARALLRAGQLASVSGAADDSLKFLERAHRLAPQERSVALLYAAARLRKGDAA
                     DAAKLLEPFSASETDPVFLETFADALMRSGQLDRARGILEKLLQEKNAGLARLFQLAD
                     LYAAAGAGTKAVEILAFLKKRMFAEKRQNEFAAQVDDVAGRHTGLAELLEFGAALYNE
                     LNRESKYFEIMIRLFDAYLERNEIRKACDALERLVDIDAYDYRNQERLEKLKGKADDA
                     FLRRVASRLTKSGTSISTADTVPQAGPASSREPAHPVTEEGQRMQALEDLIVQTEIFL
                     QYSLQSKAIDRLQKIAEMFPAEEEKNARLKTLYQTANWWPKGAPPKKVEPVRSSAAAS
                     SETGNAHTGKTGAFSADTLRDLSKISEINQKIFRQQTPRAMLNTAVSEVGEYLHASRA
                     LAVIGAAGRPPDLAAEFCGSSIKPAPGAQVVFLLAQVEKTPADELGGLILEDATGPAL
                     KDLGLRTAMGVRITDRETQQSAGMLIVGQSEPRKWKPNETYFLQAIGDQMLMSVSHTR
                     LRSLVRRMGVSDERSGLLSRSSYQSCLLTEADRARTQGTPLALTIVQIDRGAELVRQQ
                     GESPMERFLEQLARSLQPIVRQNDVAVKYTSWSLAFILPDTTLAGAQNLADKLRRAAA
                     GIRPPWDSTSITLSAGIVEAVAKPDYESEDIVTDLMNRAEFSVEEARKRGGDTVVTLD
                     APKV"
     gene            3212..>3530
                     /locus_tag="VFR42_07035"
     CDS             3212..>3530
                     /locus_tag="VFR42_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368944.1"
                     /translation="MFPTDVVIVAGARTPMSRYTGAFSDVTAIDLAAAASREAIRRSG
                     ANADEFDHAIFGNVMQTSADALYGARHVALKAGLKTETPAVTLNRLCGSGIESIAQAA
                     QRLL"
BASE COUNT          764 a         1008 c         1096 g          662 t
ORIGIN      
        1 gtggacgtag tggctggcgc gctggccgaa ctgggaacgg agcacgcgtt tgtggttcac
       61 ggcgcaggca aactggacga gatttcgctt gccgggccga cgatcatgac ggaagtccgg
      121 gccggctcgg tccgcaaatt cgaagtgacg ccagaggact tcggcaagaa gccagctccc
      181 ttggagatga ttcaaggcgg aaatctggct gagaatgcgg cgctgatccg ggacttgctg
      241 gacggagtgg tcaagacgga gggggaccgg gctcgatgcg acattgtggt catgaatggg
      301 tcggctgccc ttgttgccgc cggtgtggcc ggtaattttc gcgaaggcgc cgaattggcg
      361 gaaaaagcgc ttcgtgaagg atcggctgcg cgtaagctgg aagcccttcg gcagtatggc
      421 gacgagaaag gcgtctcgcc tgggcgtgta tgattccgca tccggtcgcg gggagttttt
      481 gcccactttt ttttcgcggt atatagttta gaagcgaagc cagcgctccg cccgcgctac
      541 ggctctactc caggctccgc aaaggcaaac cgcaggaatg gcagacgtca acaagcatct
      601 ggaccgcgcc aagaagttcc ttgagaagaa ccgtgttgag gacgcgatcg aagcgtactt
      661 gtcggtcctt gaggaagcgc catcgcatat ggaggccacg caggcgctgg gcgacctcta
      721 tgcccgccgc gagcagccgg accgggctgc cgtttactac ggaatgtttt ttgatttgct
      781 ggtcgatccc aaggacgaaa ccaaagcgct ggccatctac aaccggtttt tgaagcacgg
      841 ggcacagcag ccgcctgagc gcattgcgcg ttacgcattt ttgcaacaaa ggcaaaatcg
      901 tactgacgag gcgagcgaac aatacggcaa agcggcggag ctgttcacgg cggcggcccg
      961 ggaggaagat gcactgttct gctgggagcg cattgcccaa ctggaacccg acaatctccc
     1021 gcggcaaatc aagcttgccg acgtggcgga aaagagcggc cgcaacctgc tggcggcgcg
     1081 agcgctgctg cgtgccgggc agttggcatc ggtcagcggt gctgccgacg attcgttgaa
     1141 attcttggaa cgggctcacc ggcttgcgcc gcaagagcgc tccgtggccc tgctgtatgc
     1201 cgccgcccgc ttgcgcaagg gcgacgcggc cgatgcggcg aaattgctgg agccgttttc
     1261 cgccagcgaa accgacccgg tttttctgga aacattcgcc gacgcgctga tgcgctcggg
     1321 tcaactcgat cgcgcccgcg gcattctcga aaaattgctg caggagaaaa atgccggcct
     1381 ggcgagattg ttccaacttg cagatcttta cgcggcggcg ggagcaggca caaaagcggt
     1441 tgaaatcctg gcgtttctca agaaaagaat gttcgcggaa aagaggcaga acgagttcgc
     1501 cgcacaggtg gatgatgtag cgggaaggca cactggcttg gctgaacttc ttgagtttgg
     1561 ggcggcgctc tataacgaac tgaatcgcga gtcgaaatat ttcgaaatca tgatcaggct
     1621 ctttgacgcg tacctggaac gcaatgagat tcgcaaagcg tgcgacgcgc tcgaacgcct
     1681 ggtggatatc gatgcgtacg actatcgcaa tcaggaacgc ctggaaaaat taaagggcaa
     1741 agctgacgac gcttttctta ggcgggtggc gagtaggctc accaagtcgg ggaccagtat
     1801 ttccacagcg gataccgttc cccaagccgg accagccagc tcacgggagc cggctcatcc
     1861 agtcaccgaa gaaggccagc gcatgcaggc gttggaagac ctgattgtgc agacggagat
     1921 ttttcttcag tactcgctgc agagcaaagc gatagaccgc ctgcaaaaga tcgccgaaat
     1981 gtttccggca gaagaggaga aaaacgcccg gctgaagact ttgtatcaga ctgcgaattg
     2041 gtggcccaag ggcgcgccgc ctaagaaagt ggagcctgtg cgcagcagcg ccgcagcctc
     2101 atctgagacg ggcaacgccc acacaggaaa gaccggcgcc ttctcggccg acacgcttcg
     2161 tgacctttcg aaaatttccg aaatcaatca gaaaatcttt cgccaacaga cgcctcgcgc
     2221 catgttgaac accgccgtta gtgaagtcgg cgaatacctt cacgcgagcc gcgcgctggc
     2281 cgtaattggc gcggccggcc ggccgcccga tctggctgct gaattttgtg gttccagcat
     2341 aaagcctgct cctggcgccc aagtggtttt tctgttggcc caagtggaaa agaccccagc
     2401 cgacgaactg ggtgggctca ttttggagga cgcgaccggc ccagccctca aagatctggg
     2461 attgcgcacc gcgatgggcg ttcgcatcac cgatcgcgaa acgcagcaaa gcgccgggat
     2521 gctgatcgtc ggccaatccg aaccgcgcaa atggaagccg aacgagacgt actttctcca
     2581 ggcgattggc gatcagatgc tcatgagcgt cagccacacc cgcctccgct cgctggtgcg
     2641 ccgcatgggc gtctccgacg agcgcagcgg cctgttgagc cgtagttctt atcaaagctg
     2701 cctgctgacg gaagccgacc gcgcgcgaac gcaaggcacg ccgctggcgt tgactatcgt
     2761 gcagattgac cgcggcgcgg agctggtgcg ccaacagggt gaatcgccca tggaacgctt
     2821 tctcgaacag ctggcgcgct ccttgcaacc gatcgtgcgg caaaacgatg tcgcggtaaa
     2881 atacacttcg tggtcgctgg cgttcatatt gccggacacg acgctggcag gtgcgcagaa
     2941 cctggccgac aagcttcgcc gcgccgccgc gggaattcgg cccccgtggg actcgacgtc
     3001 catcaccctg agtgccggaa tcgtggaagc cgttgcgaag cccgactacg aaagcgaaga
     3061 catcgtgacc gacttgatga atcgcgccga attttccgtc gaagaagccc gcaaacgtgg
     3121 cggcgacacg gtagtcacgc tcgacgctcc gaaagtttga ctgctgcaac aataccctgt
     3181 gaattgcatc taacaccaat cgaggttcgc aatgtttcca accgatgtcg tcattgttgc
     3241 gggagcgcgg acgcctatgt cgcgctacac cggcgcgttt tcggatgtca ctgcgattga
     3301 cttggcggcg gccgcgtcac gcgaagcgat tcgtcgctcc ggcgccaatg ccgacgaatt
     3361 tgatcacgca attttcggca acgtgatgca gaccagcgcc gacgcactgt atggcgcgcg
     3421 gcacgttgcg ttgaaggccg gtttgaaaac cgaaactccc gcagttaccc tgaatcgcct
     3481 ttgcggctcc ggtatcgaat ccatcgcaca agccgcacag cgtttgctgc
//
LOCUS       DASTPM010000403         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_533289, whole genome shotgun sequence.
ACCESSION   DASTPM010000403 DASTPM010000000
VERSION     DASTPM010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533289"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1781
                     /locus_tag="VFR42_07040"
     CDS             96..1781
                     /locus_tag="VFR42_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0368945.1"
                     /translation="MPTPTYDAIVVGSGITGGWAAKELTEKGLNTLILEAGRPITPEK
                     DYVEHVPVWELKYRNWDVRPERAKTQFVQRECYYACDEYSNKFFVSDVDNPYTSDPGK
                     HFSWIRGRQVGGKSLTWGRQSYRWSDLDFEANLKDGIAVDWPIRYADIAPWYDYVEEF
                     AGISGQAEGLPQLPDGKFLPPMEMTCAEQDVRDAIAKHFDDRIMTIGRSAVLTRVHKG
                     RAACHYCGVCHRGCITGSYFSSQSSTLPAALKTGKLTIRPYSVVHSLIFDPATRKISG
                     IRVIDGQTKKALEFKSRIVFLNAATLESTRILLNSATTEFPNGLANSSGELGHNLMDH
                     HMGAGASGIIPGHEDKMPFGNRPNGIYVPRFRNVKTKQKDFQRGYGYQGGGSREGWSR
                     GTNMAGIGVDFKQMLRKPGPWRMTFAGFGECLPYHTNWVEVDKDKLDAWGIPTLKIHC
                     DWGDNEHVMSRDIAIQAGEMLAAAGARDVNTFQDISRPGLGIHEMGTARMGRDPKTSV
                     LNEHNQAHDVKNLFMTDGACMASAACVNPSLTYMALTARACDYAVSQMKKNEL"
     gene            1886..3589
                     /locus_tag="VFR42_07045"
     CDS             1886..3589
                     /locus_tag="VFR42_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0368946.1"
                     /translation="MAQITRSPKTYDVCVIGSGAGGGMAAKVLTEGGLNVVMLEAGPQ
                     VYPEKDYKMLMWPYELPHRGAGVGGRASEDFGEFLAPNGAWEIEGEPYTSAPGANFRW
                     FRSRIVGGRTNHWGRIALRFAPVDFQSKTRDGLGDDWPITYEDLAPYYDKVESYIGVF
                     GTKENVSSAPDGVFLPPPKPRCTEILIKKACDKLNITCIPARLAILTKAHNGRLPCHY
                     CGQCGRGCTTASNFSSSQVLIPPAMATGKLTLITGAMAREIVVGKDGKAEAVSYIDKA
                     TKTEKRVYAKAIVVAASACESARLLLNSKSTLFPDGLGNTTGNVGKYLTDSVGSDGWG
                     EIPALEKMPPHNHDGVGGMHYYVPWWKFDRKNDFPRGYHIELWGGRDMPGVGMFDGLC
                     HDEEGYGVQLKRRARQVYGTTVGFSGRGEMIPNPETYCELDPDRVDQWGIPILRFHFK
                     WSEYELRQAKDMQETFKSIIEMMGGTYKTKTRIDGQYPFGILPGGKIIHEVGTARMGA
                     DAKTSVLNGYCQTHDVKNVFVTDGAPLVTNPDKNPTLTIMALSWRASEYILDEAKKGN
                     I"
     gene            3622..>3919
                     /locus_tag="VFR42_07050"
     CDS             3622..>3919
                     /locus_tag="VFR42_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU0368947.1"
                     /translation="MSIQGISRRDVLKTLAMSAAGGSVLQVIPLEAAEMAHQMVHKQK
                     AAAPAGKYAPKYFTPKQYETLTLLCDTIIPRDEKSGGAVEAGAPEFIDLLTSENE"
BASE COUNT          901 a         1044 c         1212 g          762 t
ORIGIN      
        1 cgcaggaatt gaagctggaa attattcccg gcgcgcagca tggatgcaat cccatgcccg
       61 ccggtcttgg tgaagcctga tccttcggag aaaaaatgcc tacacctacg tatgacgcaa
      121 tcgttgttgg atcgggaatc accggcggct gggcggccaa ggagttgacg gaaaaaggcc
      181 tgaacacgct gattctggaa gccgggcggc ccatcacgcc ggagaaagac tatgtcgagc
      241 acgtgccggt gtgggagctg aagtaccgca actgggacgt ccgcccggaa cgcgccaaga
      301 cgcaattcgt gcagcgcgag tgctattacg cctgcgacga gtactccaac aaattcttcg
      361 tgagcgacgt cgataacccc tacaccagcg atcccggcaa acatttttcc tggattcgtg
      421 gccggcaggt cggcggcaaa tcgctgacct gggggcggca aagctatcgc tggagtgatc
      481 tggattttga agcgaatttg aaagacggca tcgcggtgga ctggccgatc cgctatgcgg
      541 acatcgcgcc gtggtacgac tacgtggagg aattcgccgg catcagcggc caggcggaag
      601 gtttgccgca gttgcctgac ggaaaatttc tgccgccgat ggaaatgact tgcgcggagc
      661 aggatgttcg cgacgccatc gccaagcatt ttgacgaccg catcatgact atagggcgtt
      721 cggcggtgtt aacgcgagtg cataagggcc gcgcggcttg ccactactgc ggcgtgtgtc
      781 atcgcggttg tattaccggg tcgtatttca gcagccaaag ttccacgctg ccggcggcgc
      841 tgaaaacagg gaagctgacg attcggccgt acagcgtggt gcacagcctg attttcgatc
      901 ccgctacgcg caaaatcagc ggcatacggg tcatcgacgg gcaaaccaag aaggcgctgg
      961 agttcaagag ccgcatcgtt tttctgaacg cggcgacgct ggagtccacg cgcatcctgc
     1021 taaattcggc gacgacggaa tttcccaatg gcctggcgaa cagcagcggg gaacttggcc
     1081 ataacctgat ggatcatcac atgggcgcgg gcgcgtcggg aattattccc gggcacgaag
     1141 acaaaatgcc gttcggcaat cggcccaacg gaatttacgt gccgcgattt cgcaacgtga
     1201 aaaccaaaca gaaagatttt cagcgcggct acggctatca gggcggcggc agtcgcgaag
     1261 gctggagtcg cggcaccaat atggccggca tcggcgtgga tttcaagcag atgttgcgca
     1321 agccggggcc gtggcgcatg acattcgcgg gatttggcga atgtctgccg tatcacacca
     1381 actgggtgga agtagataaa gacaaactgg atgcgtgggg aatccctacg ctgaagattc
     1441 attgcgattg gggcgacaac gagcacgtca tgtcgcggga cattgcgatc caggccggcg
     1501 aaatgcttgc cgcagcgggt gctcgcgacg tcaacacctt ccaggatatt tcccggcccg
     1561 gcttgggcat tcacgaaatg ggaactgcgc gcatgggccg cgatcccaag accagcgtgc
     1621 tgaatgaaca caaccaggcg cacgacgtga aaaacctttt catgaccgac ggcgcgtgca
     1681 tggcgtcggc tgcgtgcgtg aatccgtcgc tcacctacat ggcactgacc gcgcgagcgt
     1741 gcgactatgc cgtctcgcaa atgaagaaga acgaactata atctttcggc ggctttcggt
     1801 tgatccttcc gcttcgatgc cagagcgtcg aaggggcagg atgacaattt aaacgcggca
     1861 aatcgaatct ggcttaggac ggcgcatggc tcaaatcact cgttcgccca aaacttacga
     1921 tgtgtgtgtg atcggctcgg gagcgggcgg cggcatggct gccaaagtac tcaccgaagg
     1981 cgggctgaac gtggtgatgc tcgaggcggg accgcaggtt tatcccgaaa aagactacaa
     2041 gatgctgatg tggccgtacg agctgccgca tcgcggagcc ggcgtaggcg gacgagccag
     2101 cgaagacttt ggcgagtttc tggcgccgaa tggcgcgtgg gaaattgaag gtgaaccgta
     2161 cacttcggcg cccggcgcga actttcgctg gtttcgttcg cgcatagtgg gcggacgcac
     2221 caatcactgg ggccgcatcg ctttacgctt tgcgccggtg gacttccagt cgaagacgcg
     2281 cgatggtctc ggcgacgatt ggccgatcac gtatgaagat ctcgcgccat actacgacaa
     2341 agttgagtcg tacatcggcg tgtttggcac caaagaaaat gtttccagtg ccccggacgg
     2401 cgtttttctg cctccaccca agccgcgctg cactgaaatt ctgatcaaga aggcttgcga
     2461 caaactaaac atcacttgca ttcccgcgcg cctggcgatt ttgacgaagg cgcacaacgg
     2521 acgcttgcct tgccattatt gcggtcagtg cggtcgcggc tgcacaacgg cgtcgaattt
     2581 cagttcgagc caggttttga ttccaccggc aatggccaca ggcaagctca cgttgattac
     2641 cggagcgatg gctcgcgaaa ttgtcgtagg caaagacggc aaggccgaag ccgtttcgta
     2701 cattgataaa gcgacgaaga cggaaaaacg tgtctacgcg aaagccattg tggtcgcagc
     2761 gagcgcttgc gaatcagcac ggctgctgct caattccaag tcgacgcttt tcccggatgg
     2821 cttgggaaac acgaccggca acgtcggcaa atatttgacc gattcagtag gcagcgatgg
     2881 ctggggggaa attcccgcgc tggaaaaaat gccgccgcac aaccacgacg gcgtgggcgg
     2941 catgcactac tatgttccgt ggtggaagtt cgaccgcaag aacgattttc cgcgaggtta
     3001 tcacatcgaa ctgtggggcg gtcgcgatat gcccggcgtg gggatgtttg acggcctgtg
     3061 ccacgacgaa gaaggctacg gcgtgcagtt gaagcgccgc gcgcgccagg tgtatggaac
     3121 cacggtcggg ttttccggcc gcggcgaaat gattccaaat cccgaaacgt attgcgaact
     3181 cgatcccgat cgtgtcgacc agtggggcat cccgattttg cgcttccatt tcaagtggag
     3241 cgaatacgag ttgcgccagg ccaaggacat gcaggaaacc ttcaagtcca ttattgaaat
     3301 gatgggtgga acctacaaga ccaagacacg gatcgacggg caatatccct tcggaattct
     3361 gcccggcgga aaaattattc acgaagtggg cactgctcgt atgggcgccg acgccaagac
     3421 cagcgtgctc aacggctatt gccagacgca cgacgtgaag aacgtttttg tgactgacgg
     3481 agctccgttg gtcaccaatc ccgacaagaa tccgacgctg acgatcatgg cgctgtcgtg
     3541 gcgcgcttcc gagtacattt tggacgaagc gaaaaagggc aatatctgac cgctgcgttg
     3601 cgcggaaggc caggagagcc aatgagcatc cagggaattt caaggcgcga tgttttgaaa
     3661 acgttggcga tgagcgcagc cggcggatcg gtgctgcaag tgattccgct ggaggcggcc
     3721 gagatggcgc accagatggt gcacaagcag aaggctgctg ctcctgcggg caaatatgct
     3781 ccgaaatatt tcacgccgaa acagtacgaa acgctgactt tgctgtgtga cacgattatt
     3841 cccagggatg aaaaatccgg cggcgcggtc gaagcgggcg cgccggaatt tatcgatttg
     3901 ttgactagtg aaaatgagg
//
LOCUS       DASTPM010000404         4109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1225157, whole genome shotgun sequence.
ACCESSION   DASTPM010000404 DASTPM010000000
VERSION     DASTPM010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225157"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="rpsR"
                     /locus_tag="VFR42_07055"
     CDS             <1..435
                     /gene="rpsR"
                     /locus_tag="VFR42_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU0368948.1"
                     /translation="QTPAAPAAPQASAPAPRPEGGHAGPRREGPPGGGRREGGPGGPG
                     GPRRGKRQFFRKKKVCKFCVEKMDLIDYKRADILSQFVQERGKILPRRMTGVCARHQR
                     WLGVAIKRARNIALLPFAGSAAGSSAPRAVEAQKPQPAQPHA"
     gene            584..1030
                     /gene="rplI"
                     /locus_tag="VFR42_07060"
     CDS             584..1030
                     /gene="rplI"
                     /locus_tag="VFR42_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU0368949.1"
                     /translation="MQIILQEDVDKLGHRGDVVNVKPGYARNFILPRKLGIEATPGNM
                     KALERIRTSLAKKTATELEAAQKQAGLLNGVALKFARKTGENDQMFGSVTTADIAEGL
                     EAQGFKIDKRQIQLAEPIKLVGERDVTVKVFRDVTASIKVTVEKEA"
     gene            complement(1109..2266)
                     /locus_tag="VFR42_07065"
     CDS             complement(1109..2266)
                     /locus_tag="VFR42_07065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0368950.1"
                     /translation="MKRKYFPQFATHRGAAYAFFLIWGCALAGIPPAAHGQAATAPAV
                     THEKEYAQAEQNARKIANEWLARGIPGVTLAVTVDGRTVYAEGFGYADLEQRVPVWPG
                     TKFRIASDSKPLTAAGLMLLVEQGKIDLDAPVQKYVPSFPDKGARITPRMLAGHLAGI
                     RHYKDDEFLISRHYESVVEGLKIFENDPLVSPPGKEFHYSSYGFNLLSAVIESAAGED
                     FLSYMHERVFGPLGLRHTIEDQTTEIIEQRARPYRKLPDGTVKNAPFVDNSYKWAGGG
                     FLSTAEDLAKFGSALLQPGFLKRESLRLLFTSQKTSDGKETDYGIGWSVGTSKSGQKV
                     YAHNGGAAGGSSRLILYPDARVVVAMICNFEGEGEWSDEEVEAVGEGFEKK"
     gene            2525..3892
                     /gene="dnaB"
                     /locus_tag="VFR42_07070"
     CDS             2525..3892
                     /gene="dnaB"
                     /locus_tag="VFR42_07070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU0368951.1"
                     /translation="MSAGAILERPLPQNLDAERSILGAILLDNHALNAAIENIKPEDF
                     FLEQHRRVFNQMIALGEAQQAIDLVTLTESLHRTGELESSGGAPYLASLADGMPRVSN
                     VEHYARIVREKALLRNLIHATHNIQQRALEGEDGADAILDGAESSIFAIAEDRIKAGL
                     IPVKDIVRDNFERLEKIFREGKSITGVSTGYTELDKLLSGFQNSELLILAARPSQGKT
                     ALALNLAENISIRGGMPVAVFSLEMSKESLLQRLVASVAQIDAHKFRTGHLSREDWRR
                     MTEALGQISSAPLWIDDAGSTSVLEIGAKARRLKRDKGLSLLIVDYLQLITGRGRFNN
                     RQEEVSSISRGLKGLAKELQIPVLVLSQLTRAPEREERGPQLSDLRESGAIEQDADVV
                     MFIYRPHWAKLDSSPEERDQAEIQVAKQRNGPTDKVRFVFRSRLTRFEEAAPDAFSQF
                     APDDV"
BASE COUNT          825 a         1426 c         1029 g          829 t
ORIGIN      
        1 caaacacccg cagcgccggc cgcaccgcaa gcttcggcgc ccgcgcctcg accggagggc
       61 ggtcacgccg gcccgcgccg cgaaggccct cccggcggcg gacgccgtga aggcggcccg
      121 ggcggtcccg gcggacctcg ccgcggcaag cgccagtttt tccgcaagaa gaaagtctgc
      181 aagttctgtg tcgagaagat ggatctcatc gactacaaac gcgccgacat tctttcccag
      241 ttcgtgcagg agcgcggcaa gattcttccg cgccgcatga ccggcgtctg cgctcgccac
      301 cagcgctggc tcggtgtcgc catcaagcgc gctcgcaata tcgcgttgct gccgtttgcc
      361 ggcagcgccg cgggcagctc tgctccgcgc gctgtcgaag cgcagaagcc gcagcctgcg
      421 cagccgcacg cttaagactc cggcggacgc gagtgcgttc gggcgcgtaa cgatttgccg
      481 ggttttagag gcacagactt cagtctgtac ggtttttgta gcgccgggct tagcctggct
      541 tcagtaagtt aaaaaagttt tgcgacagaa aaggaacacg ctcatgcaga ttattcttca
      601 ggaagacgtc gacaagctcg gtcatcgcgg cgacgtggtc aacgtcaagc ccggctacgc
      661 gcgcaatttc attctcccgc gcaagctggg cattgaagct acgcccggca acatgaaagc
      721 gctcgagcga attcgcacct cgctggccaa gaaaaccgcc accgaactgg aagcggcgca
      781 gaaacaggcg ggcttgctca acggtgtcgc gctcaagttc gcccgcaaaa ccggtgaaaa
      841 tgatcagatg ttcggctccg tcaccaccgc tgacatcgcc gaaggtctcg aagcgcaggg
      901 cttcaagatc gacaagcgcc agatccagct cgccgagccc atcaagctcg tcggcgaacg
      961 cgacgttacc gtaaaggttt tccgcgacgt cactgcctct atcaaggtca ccgtcgaaaa
     1021 agaagcttaa acacctggta tttcgattct tcccaccggg cgcggcactc ccgcgccctg
     1081 tcttttgtgg ttgccggaac tccccgcgct actttttctc aaacccttcg cccaccgcct
     1141 ccacctcctc gtcgctccac tcgccctcgc cctcaaaatt gcagatcatc gccaccacca
     1201 cccgcgcatc cggatataga atcagccgcg aactccctcc cgccgccccg ccgttgtgtg
     1261 cgtacacctt ctgtcccgat ttcgatgtgc cgacgctcca tccgattccg tagtcggttt
     1321 ccttgccatc gctcgttttc tgcgaagtaa aaagcagccg cagcgattct cgtttcagga
     1381 atcccggctg cagtagcgcc gatccgaact tcgccaggtc ctccgccgtc gacaaaaatc
     1441 ctccgcctgc ccatttgtag ctgttgtcca caaacggtgc gttctttaca gtcccgtccg
     1501 gcaacttccg gtaaggccgc gcacgctgct caatgatctc tgtggtctga tcttcgatcg
     1561 tgtgccgcaa accgagcgga ccgaacacgc gttcgtgcat gtaggaaaga aaatcttctc
     1621 ccgctgccga ttcaatcacg gcgctcagca ggttgaatcc atagcttgag taatgaaact
     1681 ctttacccgg cggcgacacc agcggatcgt tttcgaaaat cttcaggccc tccaccaccg
     1741 actcatagtg ccgcgaaatc agaaactcgt cgtccttgta gtgccggatt cccgccaggt
     1801 gcccggccag cattcgcggc gtaatccttg ctcccttgtc cggaaacgac ggcacgtatt
     1861 tttgcaccgg cgcgtccagg tcgatctttc cctgttccac aagcagcatc aaccccgccg
     1921 ccgtcagcgg tttcgaatcg ctggcgatgc ggaattttgt ccccggccac accggcacgc
     1981 gctgctccag gtccgcgtag ccgaatcctt ccgcataaac ggttcgccca tccacagtca
     2041 ccgccagcgt cacgccggga atcccgcgcg ccagccactc gtttgcaatc tttcgcgcgt
     2101 tctgctcagc ctgcgcatat tccttctcgt gcgtgaccgc tggcgctgtc gccgcctggc
     2161 cgtgcgccgc cggaggaatt cccgccaatg cgcagcccca aatcaggaaa aacgcgtacg
     2221 cggcacctcg atgagtcgcg aactggggga aatattttct cttcatcggt ttctccatgt
     2281 gcgctgaaat ttttcgcgct ttatcatgcc acaccgtgct ccacaaaata tttttctcac
     2341 cgttttgcac cggcgcgcaa aattttgcac agcactccga ttgacgcgcg acccgcgcga
     2401 ctccacaatg cacccactcc ttcgttacca gctccacgga aattgcgaaa gcaatcagac
     2461 gcttgcgtgg cgacggtcgt taatgttata aaagcgaaga aaaggcgaat aagaaaactg
     2521 ccctatgtct gccggcgcca ttctcgaacg tccccttccg caaaatctcg acgccgaacg
     2581 ctccattctc ggcgccattt tgctcgacaa ccacgccctc aacgctgcca ttgaaaatat
     2641 caagccggaa gatttttttc tcgaacagca tcgccgcgtt ttcaatcaga tgatcgccct
     2701 cggcgaggcg caacaggcca ttgaccttgt caccctcacc gaatcgctgc accgcaccgg
     2761 cgagctggaa tcctccggcg gtgcgcccta tctcgcctcg ctggctgacg gcatgccccg
     2821 cgtcagcaac gtcgaacact atgcccgcat cgtccgcgaa aaagccctgc tccgcaatct
     2881 gatccatgcc acacacaaca tccagcagcg cgctctcgag ggcgaagacg gcgccgatgc
     2941 catcctcgat ggcgccgaat cctccatctt cgccatcgcg gaagaccgca ttaaagccgg
     3001 tctcattccg gtcaaggaca tcgtccgcga caatttcgaa cgcctcgaaa aaatctttcg
     3061 cgaaggcaaa agcatcaccg gcgtttctac gggctacacg gaactcgaca agcttctttc
     3121 cggcttccag aactctgagc tcctcatcct cgccgcgcgt ccttcgcagg ggaaaaccgc
     3181 gcttgctttg aacctcgccg aaaatatttc catccgcggc ggcatgcccg tcgccgtctt
     3241 cagcttggaa atgtccaagg aatctctcct ccagcgtctt gtcgcttccg tcgcccagat
     3301 cgacgcgcat aaattccgca ccggccacct gagccgtgaa gattggcgcc gcatgaccga
     3361 agccctcggc cagatctcct ccgcccctct ttggattgac gacgccggct ccaccagcgt
     3421 cctcgaaatc ggcgccaagg ctcgccgcct caagcgagac aaaggccttt ctcttttaat
     3481 cgtggattat ctccagctca tcaccggccg tggccgcttc aacaatcgcc aggaagaagt
     3541 ttccagtatc tcccgcggcc tcaaaggcct ggcaaaggaa ctccagattc cggtgcttgt
     3601 gcttagccag cttacgcgcg ctcccgaacg cgaagagcgc ggccctcagc tttccgacct
     3661 gcgcgaatcc ggcgccattg aacaggacgc cgacgtggtc atgttcatct accgcccgca
     3721 ctgggccaaa ctcgactctt cacccgaaga gcgcgatcaa gccgaaatcc aggtcgccaa
     3781 gcagcgcaac ggccccaccg acaaagtccg cttcgtcttc cgcagccgtc tcactcgctt
     3841 cgaagaagcg gcccccgacg ccttctcgca attcgccccc gacgacgtgt agcaacgctc
     3901 tgttccttgt gggagcgcag aacgctgcgc tccagcttgc cctgctccgg ctttcgcgga
     3961 ttaaacctcg ccgagctgtc aaccaacggc agtgatgccg cggcttcaaa tgtcgcgcaa
     4021 gcaacttcgc tgtgcgtttt ctaccaaccc gcgtcagatc tgacacatct acgctcatcg
     4081 ttctgcgccc cgttccccta acctcttcc
//
LOCUS       DASTPM010000405         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_821486, whole genome shotgun sequence.
ACCESSION   DASTPM010000405 DASTPM010000000
VERSION     DASTPM010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821486"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFR42_07075"
     CDS             complement(<1..281)
                     /locus_tag="VFR42_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0368952.1"
                     /translation="MAIDVRGLAPLLEIFDMPTSISFYRDALGFNVVSTSRTGTDFGW
                     ARLSLNGVEIMLNTAYEAAERPLHPDPARFAAHSDTAIYLGCPDVDAAY"
     gene            complement(336..1550)
                     /gene="argJ"
                     /locus_tag="VFR42_07080"
     CDS             complement(336..1550)
                     /gene="argJ"
                     /locus_tag="VFR42_07080"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU0368953.1"
                     /translation="MTQAPPESALPKGFRFAATACGLKKTGALDLALISSDVPASAAA
                     VFTQNLVVAAPVTLSKKNLRESKGRMRAVVVNAGNANCATGEQGELASLRTNEEAARR
                     MAMTPHELFVCSTGVIGVQLPVEKVLRALPTLARNRRASARSFTELSLAICTTDTRPK
                     TASAWFKMSGKRVHVLGCAKGAGMIHPNMATTLAFVVTDASISPALLQKTLRDVTSRT
                     FNCITVDGDSSTNDTLLVLANGAAGAPQIAKGSKAHRQFAAALEGVCGSLAQQIVADG
                     EGAQRVIEIEVRGARTESAAHKIAETIATSPLVKTAFAGGDPNWGRIFAAAGRSGVKF
                     DPALVEITMAGIKVLRKGQPLDFNERAASNKLLAEHVPIVMDLHAGKSHARYWTCDFT
                     AEYVRINASYRT"
     gene            complement(1547..2962)
                     /gene="argH"
                     /locus_tag="VFR42_07085"
     CDS             complement(1547..2962)
                     /gene="argH"
                     /locus_tag="VFR42_07085"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU0368954.1"
                     /translation="MTDRKDSKLWGGRFERAPDARFDAFQRSFAFDRRLLPYEIAVDR
                     AWAKALQPVGIFTATEVKQTLAALDKIAARAESDPAWVDSFGADAEDVHHFVEKALVE
                     ELGPLGWKLHTGRSRNELVATDFRLFVMDVALEIKLGLIGLIRALLAQAKAKLGVPMA
                     GMTHMQHAQPILVSHFLLAHAEAFTRDLTRLQSATASADACPMGSGALAGNSFAIDRA
                     AIAHGLGFSKITANSLDAVSDRDFALDYLFALSGIATHLSRLAEDFVLFASQEFSYVI
                     LPDEYSTGSSLMPQKKNPDAWELIRGKTGRITGALVALLTTLKGLPISYQRDLQEDKE
                     ALFAAHDQVADMVSVATGAIQTTGFRDDRLRAKAENPALLATEAADYLVRKGIPFRQA
                     HDLVGQVLKEAERRKKMWTDLPLSELRRISSAFDADLSASLTVEAALASKKVAGGTAL
                     DSVHAAIADLENRLPKEAAAK"
     gene            complement(3038..>3512)
                     /locus_tag="VFR42_07090"
     CDS             complement(3038..>3512)
                     /locus_tag="VFR42_07090"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368955.1"
                     /translation="NDIAARHGVGRTDLVENRLVGMKSRGAYETPGGTLLVTAHRELE
                     RLTIDRETAHLQQSLAVRYAELVYNGVWFSTQRLALDGFFNVSQQSVTGSVGLKLWKG
                     ALNVTSRKSPFSLYRADLASFTMGRYNPKDAEGFINLFALPVTVRPRAKAEGGNS"
BASE COUNT          669 a         1001 c         1130 g          712 t
ORIGIN      
        1 tcgtacgcgg catccacgtc cgggcatccg agataaatcg ctgtgtcgct gtgtgccgcg
       61 aaccgcgctg ggtcgggatg caaggggcgc tccgctgcct cataagcggt gttgagcata
      121 atctccacgc cattcaatga cagcctcgcc cagccgaaat ctgttcccgt gcgcgaagtg
      181 gaaacaacgt tgaagcccag cgcgtcacgg taaaacgaaa tcgacgtagg catatcgaag
      241 atctccaaga gcggcgcaag accgcggaca tcgatggcca tggatttctc cgcgtgaaga
      301 acgtttaaat cgcagcgatt ctagtatagg gcaggctagg tgcggtacga cgcgttaatg
      361 cgcacgtatt cggcggtgaa gtcgcaggtc cagtaacgcg cgtgggattt tccggcgtgc
      421 aggtccatca cgatcggtac gtgctcagcg aggagtttgt tggacgcggc gcgttcgttg
      481 aaatcgagcg gctggccttt gcgcagaacc ttgatgcccg ccatggtgat ttcgaccagc
      541 gcgggatcaa atttgacgcc agagcggccg gccgccgcga agatgcgacc ccagttgggg
      601 tcaccgcccg cgaatgcggt ctttaccagc ggcgaggtgg cgatggtttc cgcgattttg
      661 tgcgcggcgg attcggtgcg agctccgcgc acttcaattt cgattacgcg ctgagcgcct
      721 tcgccgtccg ctacgatctg ctgggcgagc gagccgcaca ctccttccag cgcggccgca
      781 aactgccggt gcgctttgct gccctttgcg atttgcggtg cgccagcggc gccgttggcg
      841 aggaccagta gcgtgtcgtt ggtcgagctg tcgccgtcca cggtgatgca gttgaacgtg
      901 cgggaagtca cgtcgcgcag cgttttttgc agcagcgcgg gagaaatgga cgcgtccgtc
      961 accacgaacg ccagcgtcgt agccatgttc ggatgaatca tgcccgcgcc tttggcgcaa
     1021 ccgagcacgt gtactcgctt gccggacatt ttgaaccaag ccgaagctgt cttcggacgc
     1081 gtgtcagtgg tacagatcgc cagcgagagc tccgtgaaag agcgggcgga ggcgcggcga
     1141 ttgcgggcga gagtcgggag agcacgaaga accttctcca cgggaagctg gacgccgatc
     1201 acgccggtcg agcagacaaa aagttcatgt ggcgtcatcg ccatgcgacg cgcggcttct
     1261 tcgttggtgc gcagagaagc cagctcgccc tgctcaccgg tggcgcagtt ggcgttgccc
     1321 gcgttgacca ccaccgcgcg catgcggcct ttcgattcgc gcagattttt tttggagagt
     1381 gttactggag ctgcgaccac gagattctgt gtaaacaccg cggcggcgga cgcgggaacg
     1441 tcgctggaaa tcaatgctag atccagggcg cccgtttttt tcaggccgca tgcggtagcg
     1501 gcaaaacgaa atcctttcgg cagcgcggat tctggcggag cttgcgtcat ttcgcggctg
     1561 cttccttcgg caaacgattt tcgagatcgg caatcgctgc gtgaaccgag tcgagggcag
     1621 tgccgcctgc aactttcttg gaggcaagag cagcttcgac ggtcaggcta gcggagaggt
     1681 ccgcatcaaa agctgaagaa atcctgcgca actcggacag gggcagatcc gtccacattt
     1741 ttttccggcg ttctgcttct ttcaggactt ggccgacgag gtcatgtgcc tggcgaaatg
     1801 gaattccttt acgtaccagg taatcagccg cttccgtggc tagcagcgcc ggattctccg
     1861 ctttggcgcg caacctgtcg tcgcgaaaac cggtcgtttg aatggcgccc gtcgcaacgg
     1921 aaaccatgtc cgcaacctga tcgtgcgcag cgaataatgc ttctttgtct tcctgcaggt
     1981 cgcgctggta gctgattggc agacctttca gcgtggtaag gagtgcgacg agcgcgccgg
     2041 tgatgcggcc ggtcttgccg cgaataagtt cccaggcgtc gggattcttc ttttgcggca
     2101 tgaggctgct gcctgttgaa tactcgtcgg gaagaatcac gtaagagaat tcctgcgagg
     2161 cgaagagcac gaagtcttcg gcgagacggg aaagatgcgt ggcgattccc gagagtgcaa
     2221 agagataatc gagcgcaaag tcgcggtcgc tgacggcgtc caagctgtta gcggtaattt
     2281 tggagaagcc gagaccatgg gcaatggccg cgcggtcgat ggcgaaagaa ttcccggcga
     2341 gcgcgcccga acccatgggg caggcatcgg cgctggcagt ggcgctttgc aaacgcgtga
     2401 ggtcgcgtgt gaacgcttcg gcatgggcga gcaggaaatg cgagacgaga atcggctgcg
     2461 cgtgctgcat gtgcgtcatg ccggccatgg gaacgccgag tttggccttg gcctgcgcca
     2521 gcagcgcccg aatcagtcca atcagcccga gcttgatctc caacgcgacg tccatcacga
     2581 acaaccgaaa atccgtcgct accagttcat tgcggctgcg gccggtgtgc agcttccatc
     2641 cgagcggccc tagttcctcg accagagcct tttcgacgaa gtggtgcacg tcttccgcgt
     2701 ctgcgccgaa cgaatcgacc caggcggggt cggattcggc gcgcgcggcg attttgtcca
     2761 gtgcggccag cgtttgcttc acttcggtgg cggtgaaaat accgaccggc tggagcgcct
     2821 tggcccaggc gcggtcgacc gctatctcgt aaggcagcag gcggcgatcg aacgcaaagg
     2881 aacgctgaaa cgcgtcgaaa cgcgcgtccg gcgcgcgttc gaagcgtccg ccccataatt
     2941 tgctgtcttt gcgatcggtc atgttctggc gttttgggag gtcgaagagt tcctcacttc
     3001 gttcggaatg acgattccaa ttctgggctt gagggcatca agagttgccg ccttcggcct
     3061 tggctctggg gcgcacggtg acggggaggg cgaaaaggtt gatgaagcct tccgcgtcct
     3121 ttgggttgta gcggcccatg gtgaagctag ccaggtcggc gcgatacagg gagaacggcg
     3181 acttgcggct ggtcacgttg agcgcgcctt tccacaactt taagccgacg ctgccggtca
     3241 cactctgttg cgaaacgttg aagaagccgt ccagtgctag gcgctgagtg gagaaccaca
     3301 cgccgttgta aaccagttcg gcgtagcgca ccgcgagcga ctgctgcaag tgcgctgttt
     3361 cacgatcgat cgtcaaccgt tccagttcgc gatgggcggt caccagcagc gtgccgccgg
     3421 gagtctcgta cgcgccgcgt gacttcatgc cgaccaggcg attttctacc aagtccgtgc
     3481 gtcccacacc gtgacgcgcc gcaatatcgt tc
//
LOCUS       DASTPM010000406         3706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_908122, whole genome shotgun sequence.
ACCESSION   DASTPM010000406 DASTPM010000000
VERSION     DASTPM010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908122"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFR42_07095"
     CDS             <1..718
                     /locus_tag="VFR42_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0368956.1"
                     /translation="SPDNAIYHLSAALGRLQSFSFPVNVNEITRNYFQKTASFSKGQT
                     AEDMRAVAKDPPDASAAARLSQSAYYNSLLHTTCVATLLTGGHAANALPQTARANVNC
                     RIFPGEDPESVRKTLESVAADAKVTVTIVPYKDDSGNVIPTVPVPPSPLLPEVTQAEE
                     KTVQAFWPGLPVVPTMSTGATDGRFLRIAGIPTYGIACMFFEVNDNRAHGKDERVGVK
                     DFYEGVEVAYKLIRNLSAAN"
     gene            complement(800..2557)
                     /locus_tag="VFR42_07100"
     CDS             complement(800..2557)
                     /locus_tag="VFR42_07100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU0368957.1"
                     /translation="MRKFLTGVLVFAACATAGLGADDDQSKKIALLPEQPRTMEQVID
                     RVTNNENHLYGKMKEYSPLVETYIQNLKADKDLGFVPAGDKYFLGKADFSKGVGLVSI
                     SDTNSKGKKIFVGIGNFFSFAMQYLPDGFLQMVFIDTNGFDRQHYKFDYVKREFLGEV
                     RCLVFDVTPLQKSGKGRFLGRIWVEDQDFNVVRFNGGYGGAGHSSWYFHFDSWRSNVQ
                     PGLWLPTFVYSEENNLHYAMTKKLDFKAQTRIWGYNLGHTSQEQELSKILVESNVNDE
                     TATNNDLSPLQAQRSWDRQAEDNVSDRLQRLGLMAPKGEVDKVLETVVNNLEVTNNLD
                     IQPDVRCRVMTTTTLESFTLGHTIVLSRGLIDVLPDEASLAAIVAHELAHVVLGHRMD
                     SQYAFFDQLLIDDKDSFRHFGFARTPEEEASANQKGAELLSTALKNPQVAKVWQMNNA
                     TMFFQALQTRQKEIPNLISAHLGNRVPNLPNLGSAPVAAPVETAAAGDTKAAGAPKLA
                     ALPIGGRVKMDPWSDKLEMLKSKPVGVVAEREKMPFEVTPFMPYLTRYGSENAKPIAA
                     SAQQAPDGTAAADPPAAKP"
     gene            complement(2569..>3706)
                     /locus_tag="VFR42_07105"
     CDS             complement(2569..>3706)
                     /locus_tag="VFR42_07105"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0368958.1"
                     /translation="LGVGLDPNRRAPAGQEAAWKKNIGTKPIPARLAMLPEGESRAKR
                     VGISAIVQVAIVGFVLLIPLVFPEQMKTALQYSYTEIAQPITMIPVAPPPPPPKIVKV
                     NPPPQPVVEPILSPKQPHIFVAPKLNRPQPKVQTLETKAPDLNPVLQPAMIDTTQPNQ
                     PKRPKEDVKVGMMSSGNATPATVKAPVEKVQTGGFGDPTGLPGKGNPNAHLIVNAQGL
                     NTLPNGPGYGNGTGGDKGIRGTVASTGFGNGTAVPPQGGGKKGSVQAGGFADQNAAVA
                     EAPKKTSGNDGPADTAVVVLDKPRPEYTAEGRSLRLEGDIVLDVVFQANGHVQVNRVI
                     SGLGHGLDESASKAAQQIKFKPALREGQPVDYPARVRIEFRLAY"
BASE COUNT          708 a         1183 c          972 g          843 t
ORIGIN      
        1 gtccccggac aacgccattt accatctatc cgcagcgctc ggccgtttgc aaagtttttc
       61 gtttccggtg aacgtaaatg aaatcacccg caactacttc cagaaaacgg catcgttctc
      121 caagggccag accgcggaag acatgcgtgc cgtcgccaag gaccctcctg acgcgtccgc
      181 agcggcacgg ctatctcaat ctgcctatta caattctttg ctgcatacca cttgcgtcgc
      241 caccctgctg accggtggtc acgcggccaa tgctctcccg caaacggcac gtgccaatgt
      301 gaactgccgc atcttccccg gcgaagatcc ggaatccgtc cgcaaaactc tggaaagcgt
      361 tgccgccgat gcaaaagtca cggtcaccat cgttccttac aaagacgata gcgggaacgt
      421 cattccgacc gttccggtgc cgccgtcgcc gctcttgccc gaagtaacgc aagccgaaga
      481 gaagaccgtg caagcctttt ggcccggcct gcccgtggta ccgaccatgt ccaccggagc
      541 caccgacggc cgcttccttc gcatcgccgg catccccacc tacggcatcg cctgcatgtt
      601 ttttgaagtg aatgacaacc gagctcacgg caaagacgag cgcgtcggtg tgaaggattt
      661 ctacgagggc gtcgaagtcg cttacaaact gatcaggaat ctttctgcgg ccaactgata
      721 acctgcgccg agccaggcag cgccctggaa atgaaaaagc ccgtagggtt cccctccggg
      781 ccaattctcc aaacgctcgt tagggtttag ccgctggtgg atcagcggcg gctgttccgt
      841 ccggcgcttg ttgtgcgctg gccgctattg gcttggcgtt ctcggagcca taccgcgtca
      901 aatacggcat gaacggcgtc acttcgaacg gcatcttctc gcgttccgct acgacgccca
      961 cgggcttgct cttcagcatt tccagtttgt cgctccacgg atccatcttc acgcgcccgc
     1021 cgatcggcaa agccgccagc ttcggcgctc ccgcggcttt ggtgtcgccg gcggccgcag
     1081 tttccactgg cgctgcaacc ggcgccgacc ctagattcgg caagttcggc acgcggtttc
     1141 cgaggtgtgc gctaatcagg ttgggaattt ccttctgtct cgtttgcaat gcctggaaga
     1201 acatggtggc gttgttcatc tgccagactt tcgccacttg cggattcttc agagcggtgc
     1261 tgagcagttc cgcgcccttc tgattggccg acgcttcctc ttccggcgtg cgcgcaaatc
     1321 cgaagtgccg gaaggagtcc ttgtcgtcaa tcagcaattg gtcaaaaaac gcgtactgcg
     1381 aatccatgcg atggccaagc accacgtgcg caagttcatg agccacaatt gccgccagac
     1441 tcgcttcatc cggcaaaaca tcgatcaagc cgcggctcaa aacaatggtg tgccccaggg
     1501 tgaacgattc cagcgtcgtc gtggtcatca cccggcaccg cacgtccggc tggatgtcca
     1561 gattgttggt gacctccaga ttgttgacca ccgtttccag aaccttgtcc acttcaccct
     1621 tcggcgccat caagcccaag cgctgcagcc ggtccgaaac gttgtcttcc gcctggcggt
     1681 cccacgaacg ctgcgcctgc agcggggaca aatcgttgtt cgtggcggtt tcgtcgttga
     1741 cgttgctctc caccagaatc ttgctcagtt cctgttcctg cgaagtgtgc cccaggttgt
     1801 agccccagat gcgtgtctgc gccttgaagt ccagcttttt ggtcatcgcg tagtgcaggt
     1861 tattttcttc gctgtacaca aacgttggca gccacaatcc cggttgcacg ttcgagcgcc
     1921 agctatcaaa gtggaagtac cagctggaat gtcctgcgcc gccgtatccg ccgttgaatc
     1981 gcaccacgtt gaagtcctga tcttccaccc agatgcgccc gaggaaacgg cccttgcctg
     2041 acttctgcaa cggcgtcacg tcgaatacca ggcaacgcac ttcgcccagg aattcgcgct
     2101 tcacatagtc gaacttgtag tgctgacgat cgaacccatt ggtgtcgatg aacaccattt
     2161 gcaggaatcc gtccggcaga tactgcatgg cgaacgaaaa gaaattccct atccccacaa
     2221 aaatcttttt gcccttgctg ttggtatcgg aaatagaaac caggcccaca cccttgctga
     2281 aatccgcctt gccgagaaag tatttgtcgc cggccggaac gaagcccaga tccttgtccg
     2341 ccttcaggtt ctggatatag gtctccacca gcggggaata ttccttcatt ttcccgtaaa
     2401 gatggttttc attgttggtc acgcggtcga tgacttgttc catcgtgcgt ggctgttcgg
     2461 gcagcagcgc gatcttcttg gactggtcat cgtctgcccc cagtcccgct gtggcacatg
     2521 cggcaaaaac aagcacacct gtcagaaact tgcgcatctc acttctcctc agtaggccag
     2581 tcggaattca atgcgaacgc gagccgggta gtccacaggc tggccctcgc gcaaggccgg
     2641 cttgaacttg atttgttgcg cggctttgga cgcggattca tccaatccat gtcccaaacc
     2701 gctgattact cgattcacct gcacgtggcc atttgcttga aaaaccacat ccagaacgat
     2761 gtcgccttcc aaccttagac tgcgtccttc cgctgtgtac tccggccgcg gtttgtccag
     2821 gacgaccaca gccgtgtccg cgggaccgtc gttaccggac gtcttcttgg gagcttctgc
     2881 gaccgcggca ttctggtccg caaaccctcc cgcctgcacc gacccctttt tcccgccgcc
     2941 ctgcggaggc actgcggttc cgttaccaaa ccctgtgctg gctactgtgc cgcgaattcc
     3001 cttgtcgccg ccggttccgt tgccatagcc aggtccattc ggcaaagtgt tcaatccctg
     3061 cgcattcacg atcaggtgcg cgttcggatt tccctttcca ggcagcccag ttggatcacc
     3121 gaagccaccc gtctgcactt tttccaccgg cgccttcacc gttgctggcg tcgcatttcc
     3181 gctgctcatc atcccgacct ttacatcttc cttgggccgc ttcggctgat tcggttgcgt
     3241 ggtatcaatc atcgccggct gcaaaaccgg attcaggtca ggcgctttcg tttccagagt
     3301 ttgcacctta ggctgtggtc ggttcagttt cggcgccaca aaaatatgcg gctgcttcgg
     3361 actcagaatc ggttccacga ccggttgggg cggcgggttc accttcacga ttttcggcgg
     3421 cggcggcggc ggggcgaccg ggatcatagt gatcggctgc gcgatctcgg tgtaggaata
     3481 ctgcagcgcg gtcttcatct gctccggaaa aacaagaggt atgagcagca caaatcccac
     3541 gatggcaacc tgcacaatgg cgctgattcc cacacgcttc gctcgcgact cgccttccgg
     3601 caacatcgcc aaacgagccg gaatcggctt cgttccaata ttcttcttcc aggcggcctc
     3661 ttgacccgca ggtgcgcggc ggttcgggtc aagtccaacg cccaag
//
LOCUS       DASTPM010000407         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_244948, whole genome shotgun sequence.
ACCESSION   DASTPM010000407 DASTPM010000000
VERSION     DASTPM010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244948"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFR42_07110"
     CDS             complement(<1..563)
                     /locus_tag="VFR42_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0368959.1"
                     /translation="MAERIDSHHHLWRYNEQQYDWIGAGMKAIARDFLPSDLQAEIAS
                     RGIAATVVVQARQSLTETEWLLATAAQSDFIRAIVGWAPIADASFTATLERLGEHRKL
                     KGLRHVIQSEPDDEFILRADFNEGIKRLAPSGLTYDILIYEKHLPAAIKFVDRHPNQI
                     FVLDHIAKPRIAEKLLQPWCGNLFELA"
     gene            complement(575..1966)
                     /locus_tag="VFR42_07115"
     CDS             complement(575..1966)
                     /locus_tag="VFR42_07115"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEU0368960.1"
                     /translation="MKQKLICGLLILSCFATVFCCRLGAQSKANSSDGDLLAARPPMG
                     WNSWDGYGTTINEEQFKANTKWMADNLRASGWEYAVVDMEWFVTNPTPEGNSKSSLYA
                     LDANGRYIPAENRFPSSAGGKGFKPLGDYVHSLGLKFGIHILRGIPKKAVEQNLLITG
                     SNDHAADAADTDETCPWNPDNFGANAAKPAAQAYYDSIASLYASWGVDLIKVDCISSH
                     PYRGEEIRMISEALRKTGRPMVLSLSPGAAPLEKIDEMRKYAQMWRISDDIWDIWHSD
                     VPYPQGLGDQFENVLKWAGKSQPGHWPDADMLPLGYLGPAPGWGKPRQTRLTRYEQQT
                     LMTLWAIFPSPLMIGGELTATGSWTRSLLTNAEVVGMNQHSTGNRPAIVTDKLVVWVA
                     DSTQGKGTYLAVFNRGNELAAIHYAWKELGLTGGKYALRDLWQQADAGNSNDLTAKLL
                     PHACLLYRATKVD"
     gene            complement(1973..2557)
                     /gene="pcaG"
                     /locus_tag="VFR42_07120"
     CDS             complement(1973..2557)
                     /gene="pcaG"
                     /locus_tag="VFR42_07120"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEU0368961.1"
                     /translation="MRDAANQVPTASQTVGPFFSIGLSPHNPNNFVPAQIPAGTKVVT
                     LRGRVLDGDGSVVPDAVLEIWRADETGLYSDASSSRSHDDRGVPSGFARIPTNDHGEF
                     AFQTIKPGAVQGAKGEIHAPHLAVLVFMRGLQKHLLTRIYFPDEAANEKDAVLAAVPA
                     ERRNTLIAVPDGNQNELHWDIRLQGDGETVFFEA"
     gene            complement(2558..>2775)
                     /locus_tag="VFR42_07125"
     CDS             complement(2558..>2775)
                     /locus_tag="VFR42_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368962.1"
                     /translation="GAGLLSRLVTQMYFPGDPLLAFDPIFNCVADERAQQRLISSFDW
                     ENTLPELSLGYRFDIVLRGREATPMEK"
BASE COUNT          564 a          819 c          767 g          625 t
ORIGIN      
        1 ttcgccagtt cgaacagatt gccgcaccat ggctgcagta gtttttccgc gattcgtggc
       61 ttggcgatgt ggtcgagcac aaaaatttgg ttggggtggc ggtccacaaa ttttatggcc
      121 gccggcaagt gtttttcgta aatcaaaatg tcgtaagtca agccactagg agcaagtctc
      181 ttgattcctt cgttgaaatc agcacgcaga atgaactcgt catccggctc ggactgaatg
      241 acgtgacgca atcctttcaa cttgcggtgc tcgcctagac gctccaaggt tgcggtaaac
      301 gaagcgtcag cgatgggcgc ccaaccgacg attgcacgta tgaaatcgga ttgtgccgcc
      361 gttgccagta gccattcggt ttcggtgagg gattgacggg cctgaacgac cacagttgcc
      421 gcaatgccgc gtgatgcaat ctccgcctgc agatcactcg gcaaaaaatc gcgcgcgatc
      481 gctttcatgc ccgcgccgat ccagtcgtac tgctgttcgt tgtaacgcca caaatggtga
      541 tgcgagtcga ttcgctcggc catggtcggc ggtttcagtc tactttcgtg gctcgataga
      601 ggaggcaggc atgcggcagc agtttcgctg tcaggtcgtt cgagttgccc gcatcggctt
      661 gctgccacaa atcgcgcagt gcgtacttgc cgccggtaag cccgagttct ttccaagcgt
      721 aatggatagc ggccaactcg ttgccgcggt taaaaacggc gaggtacgtt cctttgcctt
      781 gagtggaatc cgccacccac actacgagtt tgtcggtaac gatcgcggga cggttgccgg
      841 tcgaatgctg gttcatgccg acgacttcgg cgttggtcag caaagacctg gtccacgagc
      901 ccgtcgcggt aagttctccg ccaatcatca gaggcgacgg gaaaatcgcc cacaacgtca
      961 tcaacgtctg ttgttcgtag cgcgttaggc gcgtttgccg cggcttgccc cagcccggcg
     1021 cgggccctag gtaaccgagc ggcagcatgt ccgcatcggg ccagtggccg ggctgcgatt
     1081 tccctgccca cttcaaaacg ttttcgaact ggtcgcccag cccctgcggg tacggcacat
     1141 cgctgtgcca gatgtcccag atgtcgtcag agatgcgcca catctgcgcg tacttgcgca
     1201 tctcgtcgat tttttccagc ggcgccgccc cgggcgaaag actcaacacc atcggccggc
     1261 cggttttgcg aagagcttcg cttatcatgc gaatttcttc gccgcgatac ggatggctgc
     1321 tgatgcaatc cactttgatc agatcaacgc cccagctcgc atacagcgat gctatggaat
     1381 cgtaataggc ctgagcggcg ggcttggcag cgttggcgcc gaaattgtct ggattccatg
     1441 gacacgtctc atccgtatcc gccgcgtcgg cggcatgatc attcgagcct gtaatcagca
     1501 ggttctgctc cactgctttc ttaggaattc cccgcaaaat atgaatcccg aacttgagac
     1561 cgagggaatg cacatagtcg ccgagtggtt tgaatccctt tccgcctgcc gaggaaggaa
     1621 agcggttttc cgccggaatg tatctgccat tcgcatcaag cgcgtacaac gatgacttgg
     1681 agttcccttc cggcgtggga ttcgttacaa accattccat gtccacaacc gcgtattccc
     1741 agccggacgc tctcaggttg tcggccatcc acttggtgtt ggccttgaac tgttcttcgt
     1801 tgatggtggt tccgtagccg tcccaactgt tccaacccat cggcggccgg gccgcaagca
     1861 aatcgccgtc gctgctgttc gccttggatt gggcgccgag cctgcaacaa aaaaccgtcg
     1921 caaagcacga cagtataaga aggccgcaaa tcagtttctg tttcatgcgc tgtcacgcct
     1981 cgaagaaaac agtctcgcca tctccttgca accgaatatc ccagtgcaat tcattctgat
     2041 tgccgtcagg cactgcgatc aaggtgtttc gtcgctctgc gggtacggcc gcgaggacgg
     2101 cgtctttttc attcgccgct tcatccggaa aataaattct cgtcagcaga tgtttctgca
     2161 gtccgcgcat gaacaccagt accgccagat gcggcgcgtg gatttcccct ttggcacctt
     2221 ggaccgcgcc cggcttgatc gtctgaaatg caaactcgcc gtgatcgttc gtcggaattc
     2281 ttgcgaagcc gctcggcact ccacggtcgt cgtgactacg actggagctc gcgtcgctgt
     2341 acaagccggt ctcgtccgca cgccatattt ccaggacggc gtcgggcaca acggagccgt
     2401 ctccgtccag cacgcggccg cgcagtgtaa cgaccttggt gccggccggg atttgcgccg
     2461 gcacaaaatt gttgggattg tgcggactca gcccgatgga aaagaacggg cccacagttt
     2521 gggaagcggt cggaacctgg tttgcagcgt cgcgcattca cttctccatc ggcgtagctt
     2581 ctcgcccgcg cagcacaata tcaaaccgat accccagcga aagttcaggc aaggtgttct
     2641 cccaatcgaa cgaagagatc aacctctgct gggcacgttc gtccgcgacg cagttgaaaa
     2701 taggatcaaa cgccagcagc ggatcgccgg gaaaatacat ttgcgtcacc aaccggctta
     2761 ataatcccgc tccga
//
LOCUS       DASTPM010000408         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1369487, whole genome shotgun sequence.
ACCESSION   DASTPM010000408 DASTPM010000000
VERSION     DASTPM010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369487"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VFR42_07130"
     CDS             <1..1977
                     /locus_tag="VFR42_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0368963.1"
                     /translation="AGTHFFNPPRYLKLVEIIPGPKTSASVVETLSEFCDRRLGKGVV
                     VAKDTPNFIANRIGTFSVLNALRLMNELDMTVEEVDACTGPAVGWPKSATFRTTDIVG
                     LDVLAHVVRNIYETAPNDESRERYKVPALVEEMIKRGWLGDKTGQGFYKKVKGEGEKE
                     ILTLDVHTMEYRPRQKAKFASIDAGKSIDDTRQRLRALVGPVLQGQKGDKAQQFLWSA
                     ISETCLYAARRVPEISDNLVDVDHAMRWGFGWELGPFEMVDALGLNAFVTNVRKENRP
                     VPELMDKVLAAGRKSFYQSDQGVTTVFDLGAGSRKVEEPAGAIILKSLKDAGKEVDRN
                     AGASLVDLGDGVVCCEFHGKMNAIGADLIAMMHKGLKRLDTDFDAMVIANQAVNFSVG
                     ANLMLVLVGAQEQEWDELHLAVKQFQNVNLAIKYAKKPVVAAPQGMALGGGCEISLHA
                     ARIHSAAEAYVGLVEAGVGLIPGGGGTKEMLIRANENAAGGEDLDLFHALKPIFENVA
                     MAKVGTSAEECRELGYFRREDRYSMNTQRLVADAKETALSLLRSGRKPAAASWQEGAQ
                     TTQIKVLGEQFLAGAKLAIHMLVRGGYASEYDAVVARKLANILAGGALTSPQLVSEEY
                     VLELEREAFVSLLGEKKTQERIAFTLKTGKPLRN"
     gene            2409..3236
                     /locus_tag="VFR42_07135"
     CDS             2409..3236
                     /locus_tag="VFR42_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEU0368964.1"
                     /translation="MKNLEPVLTGPLFVEIRRELVSLLQGLNEEEWGRPTAAGKWNVK
                     DVALHILGGDVGNLSRRRDGFSLTADLSSYQKLVAFINEINASWVAAGQRMSPRVLID
                     LTEHVGLQADKYFAGLDSHEMGGAVSWAGDGPMPVWFDVAREYTERWHHQQQIRDAAG
                     RPGLYERRLFEPVLDTFVRGLPHTFRHVEAVRGIVVQLNVKGALQKTWSLVRADGGWK
                     LFDGTERRKDAAVAIEAEDAWKIFTRGIRGGEAERRAELSGDRRLGEKVLQMVSVIA"
BASE COUNT          731 a          726 c         1173 g          661 t
ORIGIN      
        1 gctggcacac acttctttaa cccgccgcgt tacttaaagt tggtcgagat cattcccggg
       61 ccgaaaacgt ccgccagcgt cgtggaaacg ctgagcgagt tttgcgaccg gcggctcggg
      121 aaaggtgtgg tggtggcgaa agacacgccg aactttatcg ccaatcgcat cggcacgttt
      181 tccgtgctga acgcgctgcg tctgatgaac gagctcgaca tgaccgtgga agaggtggac
      241 gcgtgcaccg gtcctgcagt gggctggccg aaatccgcga cctttcggac gacggacatc
      301 gttgggctgg atgtgctggc gcacgtggtg agaaacattt acgagaccgc gccgaatgac
      361 gagtcgcgcg agcgctacaa agtgccggcg ttggtcgaag aaatgatcaa gcgcgggtgg
      421 ctgggcgaca agacggggca gggcttctac aaaaaagtaa aaggcgaagg cgaaaaagag
      481 attttgacgc tcgacgtgca tacgatggag taccgcccgc ggcagaaggc gaagttcgcg
      541 tccatcgacg ccggcaagtc cattgacgat acgagacaac ggttgcgggc gttggtgggg
      601 ccggttctgc aagggcagaa aggcgacaag gcgcaacagt tcttgtggag cgcgatttcc
      661 gaaacgtgtt tgtatgcagc caggagagtt ccggaaattt cggacaatct ggtggatgtc
      721 gaccacgcga tgcggtgggg tttcggctgg gagctgggtc cgtttgagat ggtggatgca
      781 ctcggcttga acgcgttcgt tacgaacgta cgcaaggaaa atcggcccgt gccggagttg
      841 atggacaagg ttctggcggc tggccgcaag agcttctacc aatcggacca gggagtcact
      901 acggtttttg atcttggcgc cggcagcagg aaagtggaag agccggcggg tgcaatcatt
      961 ttaaagtcgc tgaaagatgc gggaaaggaa gtggaccgga atgcgggcgc cagcctggtg
     1021 gatttgggcg atggcgtggt gtgctgcgag ttccacggca agatgaacgc cattggcgcg
     1081 gacttgatcg cgatgatgca caaagggttg aagcgtctcg acacggactt cgacgcgatg
     1141 gtgattgcga atcaggcggt gaatttctcc gttggcgcga acctgatgct ggtgctggtt
     1201 ggcgcgcaag aacaggaatg ggacgaactg cacctggcgg tgaagcagtt ccaaaatgtg
     1261 aatctggcga tcaagtacgc gaaaaagccg gtggttgcgg ctccgcaagg catggctctg
     1321 gggggcggct gcgaaatcag cttgcatgcc gcgcgaattc attccgcggc cgaagcatac
     1381 gtgggattgg tggaggccgg cgtcggattg attcccggcg gaggtggaac gaaagagatg
     1441 ctcattcgcg ccaatgaaaa tgcggcgggt ggggaagact tggatttgtt ccatgctttg
     1501 aaaccgattt tcgagaacgt tgcgatggcc aaggtcggaa ccagcgcgga agagtgccgc
     1561 gaactgggtt atttccggcg cgaagaccgg tattcgatga atacgcaacg tctggtggcg
     1621 gacgcgaaag agacggcgct cagcttgttg cgttcgggaa ggaaaccggc agcggcgagc
     1681 tggcaagaag gcgcgcaaac cacacagatc aaagtgttgg gcgagcagtt tctggccggg
     1741 gcgaagctgg cgattcatat gctggtgcgc gggggatacg cttccgagta cgatgcggtg
     1801 gtggcgcgca agctggcgaa tattctggcg ggcggagcgc tgacgtcgcc gcaactggtc
     1861 agcgaggagt atgtgttgga gctggagcgc gaagcatttg tctcgctgct gggcgagaag
     1921 aaaacgcagg aacggattgc gttcacgttg aagacgggaa agccgctgcg taactaggat
     1981 gacttgcagt ttgcgccgcg gactgccgcc cgctcccggc tgcttcggac cgattgaaaa
     2041 tcaggatgcg cgggttcccc ctcgcgatgg acggctagcc atttaagttt tttttgtagg
     2101 tgtggcaaac aagagactta gattaggctc ctaatgcttt ctgaagtatg gcattttgaa
     2161 ggcatagtgg gtgtttttgg tcaagagtgc gcaagcggct gaaaagaaaa gagttgccct
     2221 tttagtgatg ccgcgaatgc ggaaccgtat gaaagagaag aacttaggct gccggtgagc
     2281 gtggagtccg tttgaattac tgtaagctta tactttaaag tatgccgtaa aagtaaataa
     2341 aagtcagggg aatttgtccg ggtgaggcgg cggtgcctct gcgcccggaa agattgccga
     2401 ggattgcggt gaaaaatttg gaaccggtat tgacggggcc gttgtttgtg gagataaggc
     2461 gggagttggt gtcgcttttg caaggcttga acgaggagga atgggggcga ccgacggccg
     2521 cggggaaatg gaatgtgaag gatgtggcct tgcatatcct gggcggggat gtggggaacc
     2581 tttcgcgacg gcgggatggg ttcagcttga cggcggattt atcgagttac cagaagttgg
     2641 tggcgtttat caatgagatc aatgcgtcgt gggtggctgc ggggcagcgg atgagcccgc
     2701 gggtgttgat tgacttgacg gaacacgtgg ggctccaggc agacaagtat tttgcaggac
     2761 tggactcgca cgaaatgggc ggcgcagtga gttgggcggg cgacgggcca atgccggtgt
     2821 ggttcgatgt ggcgcgggaa tataccgaac gttggcatca ccagcagcag atacgtgatg
     2881 cggcgggacg gccgggactt tatgaacggc gattgttcga accggtgctg gacacgtttg
     2941 tgcgggggtt gccacacacc tttcggcacg tcgaggcggt cagaggaatc gtagtacagc
     3001 tgaatgtgaa gggggcgttg caaaaaacat ggtcgctggt gcgagccgat ggtggttgga
     3061 aattgtttga tggaaccgag cggcgcaaag atgcggcggt cgccatcgaa gccgaagacg
     3121 cgtggaagat ctttacgcga gggattcgcg gcggggaagc ggagcgacgc gcggaactga
     3181 gcggcgatcg aaggctcgga gagaaagttc tgcagatggt gtcagtcatc gcgtgagcgc
     3241 gcgacatcct aaattcgcat gccaggaaga ttgcggcccg cgcgccataa g
//
LOCUS       DASTPM010000409         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_244970, whole genome shotgun sequence.
ACCESSION   DASTPM010000409 DASTPM010000000
VERSION     DASTPM010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244970"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFR42_07140"
     CDS             <1..525
                     /locus_tag="VFR42_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0368965.1"
                     /translation="AVVRSTVEPAALIPTARQLVRNLDLGVPPEFGSFAALVSSSLRA
                     RRFNLILVGFFAGAALLLAVVGLYGVMAHRVIRRTGEIGVRIALGATRANILRLVLAQ
                     GARVTAIGVALGIAGSLALTRAIRSLLFGLSPADPATFVAVALLLIAVALLACYLPAR
                     RAAKVDPNVALRYE"
     gene            583..>2628
                     /locus_tag="VFR42_07145"
     CDS             583..>2628
                     /locus_tag="VFR42_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEU0368966.1"
                     /translation="MSALLQDLRYAIRQLRESPGFAAVAVLTLALGIGANTAIFGLLD
                     SAILHALPFREPERLVNIWTTDSSGDLHSPFPAQYSALRANSQSFEQVAGMGWGETFY
                     GGDESGRRNLPTLLVSSNWLATLGIQPLLGRNFQDDEQTIGRDSVVALSYRCWRERFH
                     ADRSIVGREIILNRRPVTVVGVLPQSLGVYYQNIDVFRPLVLDSYIANGTLRVAGAVR
                     LRIVARLKEGVTLDRARSEAAGIAAGLKNPAAQVDPSGQLVVEDFGEALRNPGPTMQN
                     ARHALEILGVAALVVLLIGSANLASLQLARGLKRQREIAVRSALGCSRARLIRQLLTE
                     STLLSLCGGALGIFAARWSQEIINKAASGIVFNAAYLHVNARVFVAGLGFSFLSVLLF
                     GALPSIHATSTNLSDRLKDATLGATAGSPSRRLRNLSLVFQTALGMVLLVCFGLLWRS
                     LLHVQSAELGFDPNNVLTASVSLPASRYAAAADRVRLLHDSIERVQSLPGVVSAGTVD
                     SLPMDGAESAQLRIEPLTPGAPPIEKEIYFVSVSPGYFSTLQIPMLAGRPFLEQDSPT
                     GAPVAIVNRVFANTYFPRVSPIGHHLAFADLPGTQREIVGVVSDFRQRNPEEDLRPLA
                     YFPIVQTAPQGHWSLVLRVHSASDLGNVASELNKSLQSMDLQLFWEVGSMQKQIDDSE
                     "
BASE COUNT          452 a          823 c          730 g          623 t
ORIGIN      
        1 gccgtcgtcc gttctacggt ggagccggcc gcgctcattc ctactgcacg gcagctcgtg
       61 cgcaatcttg atctcggagt gccgcctgaa ttcggctcct ttgcggcgct agtttcttcc
      121 tcgctgcgcg ctcgtcgttt caatttgatt cttgtgggat ttttcgctgg agctgctctc
      181 ctgctcgcgg tcgtcggcct ctatggagtg atggcgcacc gagtcatccg tcgtaccggt
      241 gaaatcggcg tccgcatcgc tctcggtgcc actcgggcaa acattttgcg cctggtcctg
      301 gcacaaggtg cgcgtgtcac ggcaatcggc gtggccctgg gtattgcagg gtcgctcgcc
      361 ctcacccgag ccattcgctc tctgctcttc ggtttgagcc cggccgatcc cgccaccttc
      421 gtggcggtcg cactgcttct catcgcagtg gccttgctgg cctgttatct gccggcgcgc
      481 cgcgccgcaa aagtggatcc caacgtagcc cttcgctatg agtgagttcg ccatgaaatt
      541 cgcacggcac aagtggttac tccaccacgc cgaggttcgc ccatgagcgc gctgctccag
      601 gacctccgct acgccattcg ccagctacgc gagtctcccg gcttcgccgc cgtcgccgtc
      661 ctcactctcg cccttggcat cggcgccaac accgcgatct tcggcttgct tgattccgcg
      721 atcttgcacg ctctgccttt tcgcgaaccg gaacgtctgg tcaacatctg gaccacggac
      781 tcttccggcg acctgcatag tccattcccc gcgcagtatt ccgccctgcg cgcaaatagc
      841 cagtcgtttg agcaagttgc tggaatggga tggggcgaaa ccttctacgg cggcgacgaa
      901 tccggccggc gaaatcttcc aaccctgctg gtgagttcca actggcttgc gacacttgga
      961 attcaaccgc tcttaggccg caattttcag gatgacgagc aaaccatcgg ccgtgactcg
     1021 gttgtagctc tctcctacag gtgttggcgc gaacgctttc acgccgatcg aagcattgta
     1081 ggaagggaga tcattctcaa ccgccggcca gtcactgtcg tcggcgtctt gccgcaatcg
     1141 ttgggagtct attatcagaa tatcgatgtt tttagacctc tggtgctcga ctcttacatt
     1201 gcgaacggca cccttcgagt tgcgggagca gtgcgcttgc ggatcgtagc gcgcctcaag
     1261 gaaggagtaa cgctggatcg ggcgcgttcc gaagcagcgg gaatagctgc gggacttaag
     1321 aatcctgccg cgcaagtaga tcctagcggc caactggtag tcgaggattt tggcgaagcg
     1381 cttcgtaatc cgggcccgac gatgcagaat gcccggcacg ctctggaaat cttgggtgta
     1441 gcggcgttag tcgtgcttct gatcggttct gcgaatcttg caagcctgca actggctcgc
     1501 ggcctgaaac gccaaaggga aatcgctgtt cgctcagcgt taggctgctc ccgggcaagg
     1561 ttaatccggc agctgctcac cgaaagcacc cttctttccc tttgcggcgg cgctttgggt
     1621 atattcgctg cgcgttggtc ccaggaaatc atcaacaaag ccgcgtccgg catagtcttc
     1681 aacgctgctt atttacacgt taatgcccgg gttttcgtcg caggcctggg attttcgttc
     1741 ctgagcgtcc tgctgttcgg cgcactccct tccattcacg cgacttccac aaatctcagc
     1801 gaccgtctga aagatgccac cctgggcgca accgccggct ccccatccag gcgcttgcgt
     1861 aacctatcgc tggtttttca gaccgcactg ggcatggttc tgctcgtgtg cttcggcctg
     1921 ctctggcgca gtctcctgca cgtgcaatct gccgaactcg gcttcgatcc caacaacgtt
     1981 ttgacggcaa gcgtgagctt gccagcctcg cgctatgcgg ctgccgccga ccgagtccgc
     2041 ctgctgcacg attcgatcga gcgcgtgcaa tcgctaccgg gcgtagtgtc cgccggaacc
     2101 gtcgattcgc ttcctatgga cggtgcggaa agtgcccagc tcagaattga gccactcact
     2161 cccggcgcgc cgccaatcga aaaagagatc tactttgttt ctgtcagtcc tggctatttt
     2221 tcgaccctcc agatccccat gctcgccgga cgcccgtttc tggagcagga ctcgccaacc
     2281 ggtgccccag tcgctatcgt caatcgcgtc ttcgcgaata cctactttcc tcgggtaagc
     2341 ccgattgggc atcaccttgc cttcgcggat ttgccgggca ctcagcgcga aattgtcggc
     2401 gttgtgtccg atttccggca gcgcaatccg gaggaggatt tgagaccgtt ggcgtacttt
     2461 cccatcgtcc agacggctcc tcaagggcat tggagtctgg tgcttcgcgt gcactcggca
     2521 agtgacctgg gcaacgtcgc ttccgagctg aataagtcgc tccaatcaat ggatttacag
     2581 ctcttttggg aagttggcag catgcaaaag caaattgatg attcagaa
//
LOCUS       DASTPM010000410         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_706615, whole genome shotgun sequence.
ACCESSION   DASTPM010000410 DASTPM010000000
VERSION     DASTPM010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706615"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFR42_07150"
     CDS             complement(<1..297)
                     /locus_tag="VFR42_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368967.1"
                     /translation="MWARLRRFSALPRAAKLDFVRAALLLPLIRASLRLRGFRATQKT
                     LQLRIRRGVTALPEAAAADETKLLCRMVLAAGRYSLLQGTCLERSLALWWLLARK"
     gene            complement(272..577)
                     /locus_tag="VFR42_07155"
     CDS             complement(272..577)
                     /locus_tag="VFR42_07155"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEU0368968.1"
                     /translation="MIAFSERAVVSANVLIRFLDQESVLLNLETERYFGLDAVGTRMW
                     QVVTEASTVEAALARLVEEYDAPPETLRTDLTNLLQHLLDHGLIAMQPADVGTASPV"
     gene            complement(827..1966)
                     /locus_tag="VFR42_07160"
     CDS             complement(827..1966)
                     /locus_tag="VFR42_07160"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2012 domain-containing protein"
                     /protein_id="HEU0368969.1"
                     /translation="MKRLAYSCAFAVLAFAMMVAGLAPRGAAQDSGIAGTILDLQGNP
                     WPDLGVTVVSDQGTKFEAKTDKKGKYEFHNLRAGVYTLTVQLPNQVFQGGQVKVAAGQ
                     TVPADLNFKEIAAKNNPDYAKQVQKQEEEKQKFQGMKQHFEAGVAALDLTKQSKEKLA
                     KAPADQRDAVKQEVADNSNKAITEFEAAKAASNEKDPNLQLIMARLGDAYDAAGRTDD
                     AIAAYKRAIELKPSFAYYNNLGGIYGRANKIEDATAAYQKAAEIDPANAAQAWLNYGI
                     TLSNVGKYKEAVEPLKKATELDPKSAKAWYLLASAMIADPGIYKQTAGKIEVTPLPGT
                     AEAYQKAIDLDPSGPYGQQAKQGLDQLNQMTGGISTQVGAKKKKP"
     gene            complement(2178..2897)
                     /gene="queC"
                     /locus_tag="VFR42_07165"
     CDS             complement(2178..2897)
                     /gene="queC"
                     /locus_tag="VFR42_07165"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU0368970.1"
                     /translation="MANTSRKAVVLLSGGMDSCVCAAIARERHGAEGVALLHASYGQR
                     TESREAKAFRDIADFCGVKQRLMVRLEHFRAIGGSALTDPNIAVPENELNAPGPHGSA
                     IPVTYVPFRNAHFLSVAVSWAEAIGAGAIYIGAVAEDSSGYPDCRPEYYKVFQELIRV
                     GTRPETEIEIVTPVIALKKSEIIRKGIKLGAPLNLTWSCYQNAEEACGVCDSCLLRLR
                     AFEEAGVADPIPYRRAVSARP"
     gene            3023..3658
                     /locus_tag="VFR42_07170"
     CDS             3023..3658
                     /locus_tag="VFR42_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU0368971.1"
                     /translation="MPIDFSNAWKTGARIINQCISLIPNIVIAFIIFILSLVVASVCK
                     SLVRRVALRRQRHQGIALLLGRLVHTSIVILGFLVALSVVAPSFQASDLVKILGIGSV
                     AIGFAFQNILQNFLAGILLLLQEPFRIGDFISVTGIEGTVSDIQSRATVITTKEGRQV
                     IIPNAIIFTSPVAVERPAGQAVPEAANSATAGSWHDFFKETFRRPSSPGAK"
     gene            complement(3627..4322)
                     /locus_tag="VFR42_07175"
     CDS             complement(3627..4322)
                     /locus_tag="VFR42_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0368972.1"
                     /translation="MFITEIFKSVQGEGTRAGLPCVFVRLTGCNLRCTWCDTAYAFHG
                     GKKMSVEDVLTGVDALCGRHDGASGAEADVPLVELTGGEPLLQEEIYPLAEALVERRY
                     TVMIETSGERFVGRLPKEVIKIVDVKCPDSGEADTFDAKNLEAVDEKDEIKFVLSTRR
                     DYEFAREFTREHELAKRVRQVLYSPVFPDPNGKWEGLEPSQVVEWILADGLPVRLGLQ
                     LHKFIWHPATKGV"
     gene            complement(4339..>4566)
                     /locus_tag="VFR42_07180"
     CDS             complement(4339..>4566)
                     /locus_tag="VFR42_07180"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU0368973.1"
                     /translation="KRAVRGIIAGIDHQFLNDLEPFRTVNPSAENLAKYFYDETRRGL
                     VDLPQGAGITEVIVWETDTSSAIYRPGAPGK"
BASE COUNT          874 a         1597 c         1055 g         1040 t
ORIGIN      
        1 ctttcgcgcc agcagccacc acagcgccag tgaccgttcc aaacaagtgc cttgcagcaa
       61 ggaatatcgt ccagccgcca gcaccatccg gcacagcagt ttcgtttcgt ccgccgctgc
      121 cgcctccggc agggcggtga caccacggcg aatgcgcaac tgcagagttt tttgggtagc
      181 gcgaaagccg cgcaaccgca ggctcgctcg aatcagtggc aggagcagcg ccgcccgcac
      241 gaagtccagc ttcgctgcgc gcggcagcgc gctaaaccgg cgaagccgtg cccacatcgg
      301 cgggttgcat cgcgatcaat ccgtggtcta aaaggtgctg cagcaaattg gtgaggtcgg
      361 tgcgcagtgt ttccggcgga gcgtcgtatt cttccaccag tcgcgccagc gccgcttcca
      421 ccgtcgacgc ttccgtcacc acttgccaca ttcgcgtccc caccgcgtcc agcccgaagt
      481 accgttccgt ctccagattc aacaacaccg attcctgatc cagaaagcgg atcagcacat
      541 tcgctgaaac tacggcgcgc tcggaaaagg cgatcatagc gctgcgctta cctttcttgt
      601 agctggctgg gacgccttag tacttgatag ttgtgaatgt caccaccgca tgttctggac
      661 cgctgtaact gcccggtatc ttagccctcc ctcgcgattc tgaccactgc cggaacattc
      721 taaagcaaga gcctcgggtg ttagcggaca tgctggcagt tcgcctcttc ataaaaaagg
      781 ccggcacgtc ttgtgccggc cttggcccat gcaaatccaa aacgctttac ggcttctttt
      841 tcttggcgcc gacttgcgtg ctgattcccc ccgtcatctg gttcaactga tccagcccct
      901 gcttggcctg ctgcccgtac ggcccgctcg gatccaggtc aatcgccttc tggtacgcct
      961 cggctgttcc cggcagcggc gtcacttcaa tcttgcctgc cgtctgctta taaatgccgg
     1021 gatctgcaat catcgcagac gccagcaagt accacgcctt ggcgctcttc ggatccagtt
     1081 ccgtcgcttt cttcaacggt tcgacggctt ccttatactt gcccacgttt gacagcgtaa
     1141 tgccgtagtt tagccacgcc tgcgccgcat ttgcaggatc gatttccgcg gccttttgat
     1201 acgccgccgt ggcatcctca atcttgttcg cgcgcccata aatgccgccc aaattgttgt
     1261 agtaagcgaa tgacggcttc agttcgatcg cgcgcttgta cgcggcaatc gcatcgtccg
     1321 tgcgccccgc cgcatcatac gcatcgccca accgcgccat gatcagctgc aggttcggat
     1381 ccttttcatt cgaagcggcc ttcgccgctt caaactccgt aatcgctttg ttgctgttgt
     1441 cggccacttc ctgcttcacc gcgtcgcgct ggtccgcagg tgctttggcc agtttctctt
     1501 tggactgttt ggtcagatcc aatgccgcga cgcccgcttc gaagtgctgc ttcatccctt
     1561 gaaacttctg cttctcctct tcctgctttt gcacctgctt cgcgtaatcc ggattgttct
     1621 tcgccgcgat ttccttgaag ttcaagtcgg ccggaacggt ctgccccgcc gccaccttca
     1681 cctgcccgcc ctgaaacacc tggttcggca attgcaccgt cagcgtgtac acacccgcgc
     1741 gcaggttgtg aaattcatat tttcccttct tgtcggtctt cgcctcaaac ttggtgccct
     1801 ggtcgctcac caccgtcaca cccaggtccg gccacggatt cccctgcaaa tccaagatcg
     1861 ttcccgctat tccgctgtct tgcgctgcgc cacgcggcgc cagccccgcg accatcattg
     1921 caaacgccag cacggcgaat gcgcacgaat atgccagtct cttcatgctc tgatctcctt
     1981 ttacgcccgt atccactaac ctgacccggc gcactgacgc acatgtctta gaaaacgaag
     2041 cttctgccca aagcttccgc gtcccccggg tctgtccagc cttttcgcaa gtaacgaccg
     2101 gaaactcccc ctgcgcggct ttattatcac ctatccggcg gtaattccaa gtaccgcagt
     2161 cccctgagcc cctgcaatta cggtcgcgcg gaaaccgccc tccggtaggg aatcggatcg
     2221 gccactcccg cctcttcaaa cgctcgcagt cgcagcaaac agctgtcgca cacgccgcaa
     2281 gcctcctcgg cattctggta gcacgaccaa gttagattga gtggcgcgcc aagcttgatg
     2341 cccttgcgga taatctcgct tttcttcagc gcgatcaccg gcgtaacaat ttcgatctct
     2401 gtttccggcc gcgtccccac gcgaatcaat tcctgaaaca ctttgtaata ctccggccgg
     2461 caatccgggt atcccgaact gtcttccgct accgcgccaa tgtaaatcgc gcccgcgcca
     2521 atcgcctccg cccagctcac cgcgaccgaa agaaaatgcg cattgcgaaa cggcacatac
     2581 gttaccggaa tcgcgctgcc atgcggccca ggggcattca attcattttc gggaaccgcg
     2641 atgttcggat cggttagcgc cgacccgccg atcgctcgaa aatgctccag ccgcaccatc
     2701 aagcgctgct ttacgccaca aaaatccgca atgtcgcgga acgctttcgc ttcgcgcgac
     2761 tctgtccgct gcccgtagct cgcatgcaac agcgccacac cctccgcgcc atgtcgctcg
     2821 cgcgcgatcg ccgcgcacac acacgaatcc atgcccccgc tcaacagcac cactgctttt
     2881 ctagaggtat tcgccatctc tctaaattat aagcctcgcc gcctgcccca gaaagcctca
     2941 gggtttatct gcatggcatc ccgccgatag cggcggttat ccttgctcta gccgccctca
     3001 cgtttcagca caggagggca gcatgcccat cgatttctcc aatgcctgga aaaccggcgc
     3061 gcggatcatc aatcaatgta tctcgctgat tcccaacatc gtcatcgcct tcatcatttt
     3121 tattctgtcc cttgtggtcg cctccgtatg caaatcgctg gtgcgccgcg tcgctcttcg
     3181 ccgtcaaaga catcaaggca tcgccctact ccttggccgc ctggtccaca ccagcatcgt
     3241 cattctcggc tttctcgttg ccttgtccgt agtggcaccg tcttttcaag cctctgacct
     3301 agtcaaaatt ctcggcattg gcagcgtggc cattggtttc gcattccaga acatcctgca
     3361 gaatttcctc gccggcattc tcctgctgct gcaggagccg tttcgcatcg gcgatttcat
     3421 cagcgttacc ggtatcgaag gcacggtctc ggacattcaa tcccgcgcta ccgtcatcac
     3481 taccaaggag ggacgccagg tcatcattcc taacgcaatt atcttcacca gtccggtcgc
     3541 cgtggagcgt ccagcaggcc aagccgttcc cgaggcagca aactcagcca ccgctggctc
     3601 ctggcacgat tttttcaagg aaaccttcag acgcccttcg tcgccgggtg ccaaataaac
     3661 ttgtgcagtt gcaatcccag tcgcaccggc agcccatccg ccaggatcca ctcgaccact
     3721 tgacttggct ccagcccttc ccattttccg ttcggatcgg gaaacacagg cgaatacagc
     3781 acctgcctca cgcgtttcgc caattcgtgc tcccgcgtaa attcccgcgc aaactcataa
     3841 tctcgccgcg tcgacaatac aaatttaatt tcatccttct cgtccaccgc ttccagattc
     3901 ttcgcgtcaa acgtatccgc ttctcccgaa tccggacact tcacatccac aatcttgatc
     3961 acttccttcg gcagccgccc cacaaagcgc tctccgctcg tttcaatcat cacggtatac
     4021 ctgcgctcca cgagcgcctc cgccagtgga taaatctctt cctgcagcag cggctcgcct
     4081 cctgtcagct ccaccagcgg cacatccgct tccgctccgc tcgcgccatc gtgtcgcccg
     4141 cacagcgcgt ccacgccagt gagcacatcc tccacgctca tcttcttccc gccatgaaac
     4201 gcatacgccg tgtcgcacca ggtgcaacgt aaattgcatc ccgtcaaacg cacaaacaca
     4261 cacggcagtc ccgcgcgcgt cccttcgccc tgcaccgact tgaatatttc tgtgatgaac
     4321 atcgagaaga accagctatt actttcctgg ggcacctggc cggtaaatcg cgctcgacgt
     4381 atctgtctcc cacacgataa cttccgtaat ccccgcgccc tgcggcagat cgaccaaccc
     4441 gcgccgagtc tcatcgtaaa aatatttcgc cagattttcc gccgaaggat tcactgttcg
     4501 aaatggctcc aggtcattca agaattggtg atcgatcccc gcaataatcc cccgcaccgc
     4561 ccgctt
//
LOCUS       DASTPM010000411         5211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_43390, whole genome shotgun sequence.
ACCESSION   DASTPM010000411 DASTPM010000000
VERSION     DASTPM010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43390"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..190)
                     /locus_tag="VFR42_07185"
     tRNA            complement(116..190)
                     /locus_tag="VFR42_07185"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Cys,seq:gca)
     gene            complement(247..1266)
                     /gene="galT"
                     /locus_tag="VFR42_07190"
     CDS             complement(247..1266)
                     /gene="galT"
                     /locus_tag="VFR42_07190"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU0368974.1"
                     /translation="MPELRKDPITGRWVIISTERGKRPMDFLRESVIPKDGKNCPFCP
                     GNEAKTPNEVLVYGRNGGSANSAGWSIRVVPNKFPALGIEGNLDKEGEGLFDKMNGIG
                     AHEVIIESPDHSSTLATLSERALEDVLWAYRDRMLDLKNDKRFRYVLIFKNHGEAAGA
                     TVEHTHSQLIALPIVPRRVREEVDSCWHYYDEKERCIFCDIIRQEMETGERVIGENEN
                     FITLAPYAPRFPFEMWLLPKAHGSSYENNQSSMYASLARMMKDTLMRMDAVLDRPAYN
                     FMIHTSPIGEEINDHYHWHVEIIPKLTKVAGFEWGTGFYINPTPPEESARFLREARIT
                     SPVKK"
     gene            complement(1370..1876)
                     /locus_tag="VFR42_07195"
     CDS             complement(1370..1876)
                     /locus_tag="VFR42_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368975.1"
                     /translation="MFILTAAGGEEARHAGRSEAIYQVVNRRARRGLTRKVESFADAK
                     TEAKQRGAPGETFAYWYLRRQGYVFVARDYLPCGAKGEIDLAGCNGTPSHLSRCGREP
                     RGRTRRRRRNSASLGQATGGSENRSPFSVRTPRKGMPGAFEVVAIEEVPGESPVVRLH
                     KAAFYPQM"
     gene            complement(1947..3299)
                     /locus_tag="VFR42_07200"
     CDS             complement(1947..3299)
                     /locus_tag="VFR42_07200"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEU0368976.1"
                     /translation="MISTTTETKTPRLVTALPGPKAQAIVERDARFMSPSFTRDYPLV
                     AKSGRGAMVEDVDGNVFLDFAAGIAVCSTGHCHPKVVAAIQKQAAELIHMSGTDFYYE
                     SLPHLAERLVKTVPGAEDKKVFFCNSGTEAVEGAVKLARYTTKRDKMIAFYGCFHGRS
                     MGSLSLTASKSTQRKGFGGLLGGVEHIPYPYAYRCALGHTPETCGAEILETLENQIFK
                     RLFDPTEVAGIIIEPIQGEGGYVPAPKFFLQELQRICRQHGIMLIVDEVQSGMGRTGK
                     MWAFEHAGIEPDIITSAKGIASGMPLGAFIARSSVMNWKPGSHGTTFGGNPVCLAAAI
                     ATLDLLEGGMMENARKMGDYVFARTADWTKKFKIVGDVRGKGLMIGIEFVRDQRTKER
                     AGDLRNLIVDRAFHKGLLVLGAGENSLRLSPPLMIDEDQADFALKTLEECMREAEAKI
                     "
     gene            complement(3560..4999)
                     /gene="cysS"
                     /locus_tag="VFR42_07205"
     CDS             complement(3560..4999)
                     /gene="cysS"
                     /locus_tag="VFR42_07205"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU0368977.1"
                     /translation="MHELKLHNTLSGKTEPFVPQVAGEVKMYTCGPTVYDYAHIGNFR
                     TFVFQDVLRRYLKQRGLKLNHVMNLTDVDDRIIANAAAAKVGIREYTEKFVQAFFADC
                     KALSIEAPENWIRATDHIPEMVKLIERLQQKSFTYDSEGSIYYRIAKFPEYGKLSKID
                     LTGIQAGARVDNDRYEKESARDFALWKAPKAGEHFWETEIGAGRPGWHIECSAMAMKY
                     LGETLDIHTGGIDLAFPHHENEIAQSEGATGKPFARYWVHAEHLLVEGEKMSKSLGNF
                     FTMRDLYSKGYKPSALRYALASVPYRRQLNFTFDGLTQATNSVERLRTLADRLKQGKF
                     PAGSPAGFKERAAKALDEFDAGMADDLNTAVALAAAFDFLREANIAMDIGKFGQEDAK
                     AAQRFLMAFDEVFAVVADTDAEKLKELGYGPASDGLNDSEVEAKVAERQAARQRRDFS
                     KSDEIRKELAEHGIILEDSKDGSVRWKRK"
BASE COUNT         1014 a         1638 c         1351 g         1208 t
ORIGIN      
        1 ttcgatctgg agactcgggt gctcgaacca ctcgaaaatc cgctcggccc aaaagtgtta
       61 cccatgtagc cggtacaaag tgtcacccat gtgcccggtc tggaccagat tttgttggag
      121 gcgcgggtgg gaatcgaacc cacgcataaa ggttttgcag acctctccct taccacttgg
      181 gtaccgcgcc gccaatccat aattataacg aaatatgcag cgctctgtca gcggccaaac
      241 gcgaagctac tttttgacgg gactggtgat gcgcgcctcg cgcagaaacc gagccgattc
      301 ctctggcggg gtggggttaa tgtaaaaacc agttccccat tcgaagcccg ccactttggt
      361 gagcttggga ataatttcga cgtgccagtg gtagtgatcg ttaatttctt cgccgatggg
      421 agaagtgtga atcatgaagt tgtacgctgg gcgatccagc acggcgtcca tgcgcatcaa
      481 tgtgtctttc atcatgcgcg ccaagctggc gtacatcgag ctttggttgt tctcgtacga
      541 agaaccgtgt gcttttggca gcagccacat ctcaaacgga aaccgcggcg catacggcgc
      601 caaagtaatg aagttttcgt tttcgccgat cacccgctca cccgtttcca tttcctggcg
      661 gatgatgtcg cagaaaatgc agcgctcttt ttcgtcgtag tagtgccagc agctatcgac
      721 ttcttcacgc acgcgccgcg gcacaatcgg caaggcgatt agctgcgaat gagtgtgctc
      781 caccgttgcg ccggctgctt cgccgtgatt cttgaagatc agcacgtaac ggaagcgctt
      841 gtcgttcttc aggtccagca tgcggtcccg gtaagcccac aaaacatctt ccagcgcccg
      901 ctcggaaagg gtggccagcg tcgaggaatg gtccggcgac tcgatgatga cttcgtgagc
      961 gccgataccg ttcattttgt cgaagaggcc ctcgccctct ttgtccaggt tgccttcaat
     1021 acccagcgcc ggaaatttgt tcggcaccac gcggatggac cagcctgcgc tgttcgcgct
     1081 gccgccgttg cggccataca ccagcacttc gttgggcgtt ttcgcttcgt tgcccggaca
     1141 aaatggacag ttcttcccgt ccttggggat cacgctctcg cgtagaaaat ccatgggccg
     1201 ctttccacgt tccgtggaaa taatcaccca gcggccggta attgggtctt tgcgtaactc
     1261 cggcaactct gcctcctaaa accttttact gcggcttgcg aaacattcct tgaatggtgt
     1321 ttattttagc aagttcttgc ggattctgat gcctttcggg gagcggcgtt tacatctgtg
     1381 gataaaacgc cgctttatgc aagcgcacga ccggcgattc gcccggcact tcttcgatcg
     1441 ccaccacctc aaacgcaccg ggcattcctt tacgtggcgt tcggacagaa aacggcgagc
     1501 ggttctcgct gccgcctgtt gcctgtccaa gtgacgctga gttccggcgg cgccgcctgg
     1561 tcctaccacg cggttcgcgt ccgcacctcg acaaatgcga gggtgtcccg ttgcatccgg
     1621 ccagatcgat ttcgcccttg gctccgcatg gcaggtagtc tcgcgccaca aaaacgtatc
     1681 cctgccgccg caaataccaa tacgcgaacg tctcgccggg tgcgccgcgc tgttttgctt
     1741 ctgttttcgc atcggcgaac gattcaactt tccgcgtcaa accgcggcgc gcgcgccggt
     1801 tcacaacctg gtagatagcc tcgctacgac cggcatgccg cgcttcttcc ccgccggcgg
     1861 ctgtgagtat aaacaaaaat cccgtggccg ccgcaaactt ccgcaggtca gccccgggat
     1921 tttccgtgaa tcttggctat gttccgctag atctttgcct ccgcttcacg catgcactct
     1981 tccagagtct ttagcgcgaa atcggcttgg tcctcgtcaa tcatgagcgg cggcgagaga
     2041 cgtagcgagt tttcgccggc tccgagcacc agcagtcctt tgtggaatgc gcgatctact
     2101 atcaagttgc gcaggtcacc cgcgcgttcc ttggtccttt gatcgcgcac aaactctatg
     2161 ccgatcatca gccccttgcc gcgcacgtcg cccacgattt tgaacttctt cgtccaatcc
     2221 gcagtgcgcg caaagacgta gtcgcccatc ttgcgagcgt tttccatcat gccgccttcg
     2281 agcaggtcca gtgtcgcgat ggccgccgcc aggcacaccg gatttccgcc aaacgtcgtc
     2341 ccatgcgaac cgggcttcca gttcattaca ctcgaccgcg caatgaaggc gccgagcggc
     2401 atgccactgg caattccctt cgcgctggtg atgatgtccg gctcgatacc ggcatgttca
     2461 aatgcccaca tcttgccggt gcgtcccatg cccgactgca cttcatccac gatcagcatg
     2521 attccgtgct gccggcagat gcgctgcagt tcctgcagga agaacttcgg cgccggcacg
     2581 tatccgcctt caccttgaat cggttcgatg atgatgcccg ctacttcggt cggatcgaaa
     2641 aggcgcttga agatttgatt ctctagcgtc tccaggattt ccgcaccgca ggtttccggc
     2701 gtatggccca gcgcgcagcg atacgcatac ggatagggaa tgtgttctac gccgccgaga
     2761 aggccgccaa atcccttgcg ttgcgtggat ttcgacgccg tcaatgagag cgaacccatg
     2821 ctgcggccgt ggaagcagcc gtaaaacgcg atcattttgt cgcgcttggt ggtatagcgg
     2881 gcgagcttca ccgcgccttc tactgcttct gtgcccgaat tgcagaagaa aactttcttg
     2941 tcttcggcac ccggcacggt cttgacaaga cgttcggcca gatggggcag gctctcgtag
     3001 taaaaatccg tgcccgacat gtggatcaac tcggcggcct gtttctggat tgccgccacc
     3061 actttgggat ggcagtgccc cgtcgagcac accgcaattc ccgcggcaaa atccaaaaat
     3121 acgttgccgt ccacgtcttc caccatcgcg ccgcggccgc tcttggccac cagcggataa
     3181 tcacgcgtga acgaaggcga catgaacctg gcgtcgcgtt ccacaattgc ctgggcctta
     3241 gggccgggca gcgccgtaac cagacggggc gttttcgttt ccgttgtcgt cgaaatcatt
     3301 gcaatctccc ttttctcgcg ggccgttggc aaccgcggtt ccggttgttc tggtggactc
     3361 cggcgaagca acgctggagc gttggaacga gtgcgcagtt cgcattctcg ggatgtgcgg
     3421 ggcgcgccgg cttgcaagcg cactaggaca ccaaaatgct cactgccttt ttggaggggg
     3481 acttaatcta cgccgtagag gttcggacgc agttcggaag gagaagcttg ttgggccagc
     3541 cggtgcaatt tgttgtgtgt catttgcgtt tccagcggac gcttccgtcc ttactatctt
     3601 ccagaattat accatgctct gctagctctt tgcggatttc gtcgctctta gagaagtccc
     3661 gccgttgccg agctgcctgc cgctccgcga ctttcgcttc cacctcggag tcgttcaagc
     3721 cgtcgctcgc agggccataa cctaattcct tcagcttctc cgcgtcggta tctgccacca
     3781 ccgcaaagac ttcatcgaac gccattagaa acctttgcgc cgctttcgca tcttcctgcc
     3841 cgaatttccc gatgtccatc gcaatgttcg cttcgcgcaa gaaatcgaac gccgccgcca
     3901 gcgccaccgc cgtattcaaa tcgtccgcca ttcccgcatc aaactcatcc agcgcctttg
     3961 ctgcgcgttc cttgaatccc gcaggcgagc ccgcaggaaa tttcccttgc ttcagccggt
     4021 ccgcaagcgt ccgcaagcgc tccaccgaat tcgtcgcctg cgtcagcccg tcaaaggtga
     4081 aattcagttg cctccggtac ggcacgctgg ccagcgcata ccgcagtgcc gacggcttgt
     4141 atcccttgga atacaagtcg cgcatggtga aaaaatttcc cagcgacttg gacatcttct
     4201 cgccttcgac cagcagatgc tccgcgtgta cccaatagcg cgcgaacggc ttccctgttg
     4261 cgccttcgct ctgcgcaatc tcgttttcat gatgcggaaa cgccaaatca attccgcccg
     4321 tgtgaatgtc cagcgtctcg cccagatatt tcatcgccat cgccgaacac tcgatgtgcc
     4381 agccaggccg ccccgcgcca atctccgttt cccaaaaatg ttctccggcc ttcggcgctt
     4441 tccacaacgc aaaatcccgc gcgctctctt tttcgtagcg gtcgttgtcc acgcgcgcgc
     4501 ctgcctgaat ccccgtcagg tcaatcttcg acaactttcc gtattccgga aacttcgcta
     4561 tccgataata aatcgaccct tcgctgtcgt acgtgaagct tttctgctgc aagcgctcaa
     4621 tcagcttcac catctccgga atatggtccg tcgcgcgaat ccagttttcc ggcgcctcaa
     4681 tgctcagcgc cttgcagtcg gcaaaaaaag cctgcacaaa cttttcggtg tactcgcgaa
     4741 tgcccacctt cgccgccgcg gcattggcga tgatccggtc gtccacgtcg gtcagattca
     4801 tcacatggtt tagtttcagc ccgcgctgct tcaagtaccg ccgcaagaca tcctgaaaca
     4861 caaacgttcg gaaattgccg atgtgcgcgt agtcatagac cgtaggcccg cacgtgtaca
     4921 tcttaacttc gcccgccacc tgcgggacga agggctccgt cttgccgctc agcgtgttgt
     4981 gcaatttcag ttcgtgcatg tctttcgtca gccctcgacg ttacgaaaga aactccagat
     5041 tgtagctgac gcccgcaaag gcccgcaaac gagcgtcttt tgatcggcca cgctgtgaac
     5101 acacaacgca cgaaataccc gtcctatggt acagggcaca agtacagcta attgtctcgg
     5161 cacgactgga ccaactgctt cgccgcaacc gccaaaaaca acctacctta g
//
LOCUS       DASTPM010000412         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_331735, whole genome shotgun sequence.
ACCESSION   DASTPM010000412 DASTPM010000000
VERSION     DASTPM010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331735"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VFR42_07210"
     CDS             <1..882
                     /locus_tag="VFR42_07210"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0368978.1"
                     /translation="KLVPLTLFAALFLTSGCGRTQSAQADARAAKQDTPDAEPIRFVK
                     NPDTAPAFVLNDLQGKAVSLAEAKGKVVLLNFWATWCGPCRAEIPDLVDLQKRYADKL
                     EIIALATQEDDADQVRRFVLHSGINYRVAMAPDDVVREYGGIAALPTSFVIDVQGRVV
                     QKHVGLNDPTLYERELKAMLGMAVNAKVEYFEDNGQVFLKNAERATELPGVDFSKLTA
                     AQKSAALRQMNAKSCDCGCGLTLAQCRINDSACRVSLALAGQVVDQISGRAPKSNAKS
                     PAAAPNGASNSNAPPQD"
     gene            complement(898..1566)
                     /locus_tag="VFR42_07215"
     CDS             complement(898..1566)
                     /locus_tag="VFR42_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368979.1"
                     /translation="MSGTLASSAGNARIVAGGPGTRVAKKKRGCGCLGSLAGLIVLVT
                     ALLVLLNPWSLHMGGRWTPALTWHGVGTLHSSTGATYGFYMELWPYLSRGRGGYSGGH
                     QNIEGTAKICTPQGGMYPMTVSGYLKNTWLDANGKPIAFYLRSPKDASTKLNIDLHGS
                     WQGQELALEDKGNMAMSFSADGNAKGYLHGVNAPAENTTGKLRYAGESELASVCGQKD
                     KNSF"
     gene            1656..2579
                     /locus_tag="VFR42_07220"
     CDS             1656..2579
                     /locus_tag="VFR42_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEU0368980.1"
                     /translation="MNTKAAFFLLLILSASGFISPPIALCAGLIFGLATQHPYAADSR
                     AFSKFLLQASVVALGFGMNLHEVLKAGRSGFLYTALGIGFALLAGFLIGKLFSVRGTT
                     SYLISTGTAICGGSAIAAIGPILEASDEEMAVALGTVFILNSIALFIFPTIGASLQLS
                     QSQFGLWSALAIHDTSSVVGAASRYGNQALVVATTVKLARALWIVPLALATAAARHSR
                     SKVQIPWFIFLFCLAAVVNTYGPQEPRLSQMFFQLGRLGLTVTLFLIGTGISRATVKE
                     VGWRPLAQGVLLWILVGGTSLYFIRIGWIAL"
     gene            2706..3173
                     /locus_tag="VFR42_07225"
     CDS             2706..3173
                     /locus_tag="VFR42_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEU0368981.1"
                     /translation="MNRRVGIVLALLLVAGIFTVVGLSSQPTPQATRKYIDLPGKTVQ
                     APFSDAVLTGNTLYLAGRIGLDPKTGKAPDKIEDEIKLLLDGEKDVLAQAGMTMDDLV
                     YVQISCTDLSLFERFNPIYKSYFTKEMPAREFIGAASLLRGGHFELQAIAVKR"
     gene            complement(3191..3874)
                     /locus_tag="VFR42_07230"
     CDS             complement(3191..3874)
                     /locus_tag="VFR42_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368982.1"
                     /translation="MTLLRRGSFFAALSISILFLDVAGGLAQEAEKPEAPAARPQGPP
                     AEGLRVIAAQAPYEPISGKQRITWAALQTFGPESLLVGTLSAGIGTARDNPEEYGPHW
                     DGFAKRYGMRLTGIASSNTIEAGFGALWGEDPRYVRNQNLPFKKRIGNVFLLTVTARN
                     RQGKMMPAYARYIAIPGNNFLSNTWRVSSASTTNAALARTGYGFLGAIASNAWSEFWP
                     DVKRIVFKK"
BASE COUNT          822 a         1372 c         1073 g          966 t
ORIGIN      
        1 aaactcgttc cgctcaccct tttcgcggcg ctgttcctca cgtcaggctg cggacgaacg
       61 caatccgccc aggccgatgc cagggccgcc aaacaggaca ctcccgacgc cgaaccgatc
      121 cgcttcgtca agaaccctga caccgcgccg gcctttgtgt tgaacgatct ccaaggcaaa
      181 gcggtctcgc tcgccgaagc caagggaaaa gttgtcctcc tgaatttctg ggccacttgg
      241 tgcggcccct gccgcgcaga aatccctgat ctggtcgact tgcagaaacg ctacgcggac
      301 aaactcgaaa tcattgcgtt ggctactcag gaggacgatg ccgaccaggt gcgccgcttc
      361 gttcttcact ccggcataaa ttatcgtgtc gccatggcgc cagacgatgt cgtccgcgaa
      421 tacggcggta ttgccgcact cccgacgtct ttcgtgattg acgtgcaggg tcgcgtcgta
      481 caaaaacacg tcggcttgaa cgatcccacg ctttacgagc gcgaactgaa agccatgctg
      541 ggcatggccg tgaacgccaa agtcgaatac ttcgaggaca acggccaggt cttcctgaaa
      601 aatgcggaac gcgccaccga gcttccgggc gtggattttt ccaagttgac ggccgcacag
      661 aaatccgccg cccttcgcca gatgaacgcc aaatcttgcg attgtggttg cggcctcact
      721 ctcgcacagt gccgcatcaa cgacagcgcg tgccgcgtta gcctggctct tgccggccaa
      781 gtggtcgatc agatttccgg ccgcgcgccc aaatcaaatg ccaaatcccc cgctgctgca
      841 cctaacggcg cttctaacag caacgcgccc ccgcaagact agctccacca accggctcta
      901 gaaactgttc ttgtccttct gtccgcaaac cgaagccaat tcgctttcgc ctgcataacg
      961 caatttgccg gtggtgtttt ccgctggtgc gtttactcca tgcaaatagc ccttggcgtt
     1021 tccatccgcc gaaaaggaca tcgccatgtt ccccttgtct tccagcgcca attcctgccc
     1081 ctgccacgag ccgtgcagat caatgttgag tttcgtcgac gcgtccttcg gcgaacgcaa
     1141 atagaaagcg atcggttttc cgttcgcatc gagccaggta ttcttcaagt agccgctgac
     1201 tgtcatcgga tacatcccgc cttgcggcgt gcagattttc gccgttcctt cgatgttctg
     1261 gtggcctcca gagtagccgc cgcgtccgcg cgacaggtac ggccacagct ccatgtagaa
     1321 tccgtaagtc gctccggtcg aagaatgcag cgtccccact ccgtgccagg tcagtgccgg
     1381 tgtccaccgg ccacccatgt gcagcgacca tggattcagc agcaccagca gtgccgtcac
     1441 cagaacgatc agccctgcca gggaaccgag gcagccgcac ccgcgtttct ttttcgcaac
     1501 tcttgtccct ggacctcccg ccacgatccg cgcgtttcca gcggagcttg ccagagttcc
     1561 ggacattccc ttacctccca tccgttttgc gcagcactct atcacgcgcc cagccgttct
     1621 aagtcaggtc gtcgtacact agtgcgtctt ccggaatgaa tacaaaagcg gcgttcttcc
     1681 ttctcctgat tctgagcgcg agcggcttta tctcgccgcc catcgctctc tgcgctggcc
     1741 ttatcttcgg tttggccaca cagcatccct acgccgccga tagccgcgcc ttctccaaat
     1801 tcttgctgca agcctccgtt gtcgccttgg gcttcggcat gaatcttcac gaagtgctca
     1861 aggccggccg cagcggcttt ctttacaccg cgttgggaat cggcttcgcg ctgttggccg
     1921 gttttctgat cggcaagttg ttttccgtgc gcggcaccac ttcttatttg atttccacgg
     1981 gcaccgccat ctgcggcggc agcgccatcg ccgccattgg gccgattctc gaagccagcg
     2041 acgaagaaat ggccgtggct cttggcaccg ttttcattct caattccatc gccttgttca
     2101 tctttcccac gattggcgcc tcgctccagc tttcgcaatc gcaattcggc ctgtggtcag
     2161 ccctcgccat ccacgacacc agctctgtcg tgggcgctgc ttcgcggtat ggcaaccagg
     2221 ctctcgtcgt cgccaccacg gtcaagctcg ctcgcgctct ttggatcgtt ccgctggcgc
     2281 ttgccaccgc tgctgcccgg cacagccgct ccaaagttca gatcccgtgg ttcatcttcc
     2341 tgttctgtct tgcagccgta gtgaacacct atgggccgca ggagccgcgc ctttcccaga
     2401 tgttcttcca gctaggccgc ctgggtctga ccgttacgct ctttctgatc ggcacgggaa
     2461 tttcgcgtgc cactgtcaaa gaagtcggct ggcggcctct ggcgcaaggt gtcctgctct
     2521 ggatactggt cggcgggact tcgctctatt tcatccgcat cggctggatc gcgctttaga
     2581 gacagtccga aaattgtgtt ctccccgggg ccgtgcgaga ttccttcttg cagccgcgcc
     2641 gctggcacac tacacttttc cgcaagcttc gccgtcgcat tcgtgcgacc agagaaggag
     2701 cattcatgaa tcgccgagtg ggaatcgtcc tggccctgtt actggttgcc ggaatcttta
     2761 ccgttgtcgg gctttcttcg cagcccaccc ctcaagccac tcgcaaatac attgaccttc
     2821 ccggcaaaac cgtccaggcg ccgtttagcg acgccgttct taccggcaat acgctctatc
     2881 tcgccggccg catcggcctg gatccaaaaa cgggcaaggc tcccgacaaa attgaagacg
     2941 aaatcaagct gttactggac ggcgaaaagg acgttctagc acaagccgga atgaccatgg
     3001 acgatttggt ttatgtgcag atttcctgca ctgacttgtc gctcttcgag aggttcaacc
     3061 ctatctacaa atcgtatttc accaaggaaa tgccggctcg cgaattcatc ggcgctgctt
     3121 cgcttctccg cggcggccat tttgaattgc aggcgatcgc agtaaagcgg taatacagcc
     3181 cgccccgaac ctatttctta aagacgattc gtttcacatc cggccagaac tcgctccacg
     3241 cattgctggc gattgcaccc agaaatccgt aaccggtgcg cgccagcgct gcgttcgtcg
     3301 tggatgcgct gctcacccgc cacgtattcg ataaaaagtt attccccgga atcgcgatat
     3361 atcgcgcata tgccggcatc atctttccct ggcgattgcg cgccgtaact gtgagtagaa
     3421 acacattgcc gattcgtttc ttgaagggca agttttggtt gcgcacatat cggggatctt
     3481 ccccccacaa ggctccgaac cccgcctcaa tcgtgttgct ggatgcaatg ccggtgagcc
     3541 gcattccgta ccgctttgcg aatccatccc aatgcgggcc gtactcttcc ggattgtcgc
     3601 gggctgttcc gatcccggca gacagcgtcc ccaccagcaa actttccggt ccgaatgtct
     3661 gcagcgccgc ccaggtgatt ctctgcttac ccgaaattgg ttcgtacgga gcctgcgccg
     3721 caatcaccct tagaccttcc gccggcggcc cctgcggtct tgctgcaggc gcctccggct
     3781 tttccgcttc ctgcgccagg cctcccgcca cgtccagaaa aagaattgaa atggaaagcg
     3841 ctgcgaagaa actacctcgt cgaagaagtg tcatgcggga attaggccat ttccatttct
     3901 ctgattttcg cctgcgaaag cagcacgcgc atacacctat agtgacctag gcgcgggcaa
     3961 agaaaaagca gagagaacgc cctagctcga cttttctcgc tccccggctt cgcataacac
     4021 tccggaagac cgccggaccc gccgaagcag ctcgggcgat atgcccaaat aaggtctagt
     4081 ttatagtgac cgccgacaat ctaatgccca tatacgggct aacagacgcc atgcttggcg
     4141 cccaaagatc catgtaaggc aagttttatc agtaatttac ggtttagcga gagtttcaca
     4201 cactttgggg atgcttgtgc atctcactta tcc
//
LOCUS       DASTPM010000413         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_389431, whole genome shotgun sequence.
ACCESSION   DASTPM010000413 DASTPM010000000
VERSION     DASTPM010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389431"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..3241
                     /locus_tag="VFR42_07235"
     CDS             206..3241
                     /locus_tag="VFR42_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU0368983.1"
                     /translation="MARAFWLRLAWSVFACCCFLAGALADGETQPGRDAKQPIDEAYT
                     AKIKKYTTESFFTSPLVDYLPASKTVPTPEAVLGDVAGAPGILPYAKDVYKYMRMLEK
                     ASPRVKVFSIGTSEEGREMIAVALSSEANLKKMEENRALLAKLADPRTLKLNDAEADK
                     IVAQSSPIYYITGTIHSPETGAPTALMELAYRLTVDESPYIKDIRDGMITLITPVVEV
                     DGRDRMVDLYKWHLAHPGSNYPNLVYWGKYVAHDNNRDAMGVTLKLTENVMNTYAKWN
                     PQVLHDLHESVPYLYDNTIGDGPYNAWIDPILADEWNLISGRNVAEMTKFGMPGVFAH
                     GDFDTWSPGYLMFIAALHNGISRLYETFGNGGADTLERTLEPEEYARTWYKQNPPLPK
                     VLWSQRDNNNYEETGLLVSLHYFNENKRMFLKNFYLKAKRSVEKPKSEGPAAYVLPAD
                     DPRATVQLELLKVMQKQHVEISRLTSATTIALPPKKEKKPKAGEKKAEKKEEKKDEKP
                     EPTTKTFPAGSYVIRMDQPYSRIADALLDYQYWSPEDPQKTPYDDTGWTFGELYGVQV
                     SRVTDAKILEAPMEAVKELSVAAGVKGEGSIFAIENHAEPALASLRYKLKDADMQAAE
                     EEFEANGKKFAAGTVLVKGVERGQFDSAVKELGLQPVTLASAPSVKMHPLRAAHILLL
                     HTWLSTQTEGWWRLALDKLGVPYDYASTQTIAGTPDLREKYDVILFPPVGFAGSLNNI
                     VNGLPTNWGNPLPWEKTELTPNLVGKNDSTPDMRPGLSWAGVAKLQKFVNDGGVLLTV
                     MDTAQFAAQAGITTGVTSSNSDKMKIVGSVVGTRLVDGASPIAYGYGEKVAAYCDNGP
                     VFSLTSIAGSRRRRRLGAEMGERPTGRGSKNDPDFAVGRAGVEAPEETESELWEAPPM
                     TDELRHNNPRVIPPAMRARVVLRYADAKDLLISGLVQGGGEIAQHPAVVDAPLGKGHV
                     VMFSINPVWRGETQGTYSLVLNTVMNFDSLNAGRKDAEK"
     gene            3484..3762
                     /locus_tag="VFR42_07240"
     CDS             3484..3762
                     /locus_tag="VFR42_07240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEU0368984.1"
                     /translation="MLLVILRPREGQELFLEAELRALMGPTRKEEGCLRYDLYRSAEG
                     TAAYMLYEIWESRAHHTAHTKTDHFTRWNARKDSLLASRDVGFWKQIA"
     gene            3797..>4027
                     /locus_tag="VFR42_07245"
     CDS             3797..>4027
                     /locus_tag="VFR42_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368985.1"
                     /translation="MKKKWIVANLALATALAIALAPSGFAQEPEHQHHHDGAENLGTV
                     NFPVSCAPAVQPAFNRAMALLYSFEYESSLQAF"
BASE COUNT          908 a         1121 c         1251 g          747 t
ORIGIN      
        1 tgtgccgtcg gtagcgccgg tctttagacc ggttcctatt cttcgcttcg cgaccctgcc
       61 ttgaccccgc ctgccgggcg ccttggcgtt attcattttt cgtttccgct ttgagcgcgt
      121 ggcttggcaa aaacgcactg ccgctggccg ccgtctaaaa acgcagtaca atcacctgtc
      181 caatttttca tcacggagac atttcatggc ccgcgctttc tggttgagac tcgcctggtc
      241 cgtttttgcc tgctgttgtt tccttgccgg cgcccttgct gacggcgaaa cgcagccggg
      301 ccgcgatgcg aaacagccca tcgacgaggc ttacaccgcc aagataaaga agtacacgac
      361 ggaatctttc ttcacgtcgc cgctggtgga ctatcttccc gcctcgaaaa ccgtgccgac
      421 acccgaagca gtgctaggcg atgtcgcagg cgcgcccggc atcctgcctt acgccaaaga
      481 tgtgtacaag tacatgcgca tgctggaaaa agccagcccg cgcgtgaaag ttttctccat
      541 cggcaccagc gaagaaggcc gtgaaatgat tgctgtggcg ctctcttccg aagccaacct
      601 gaaaaagatg gaagagaatc gcgcgctgct cgcgaaactc gccgatccgc gcacgctgaa
      661 actcaacgac gcggaagccg acaaaatagt cgcgcaatcc agtcccatct actacatcac
      721 cggaacgatt cactcgccgg aaaccggagc gcccaccgcg ttgatggagc tcgcttatcg
      781 cctcaccgtc gacgaaagtc cctacatcaa ggacatccgc gacggcatga tcacgctcat
      841 cactcccgtc gttgaagtcg acggtcgcga ccgcatggtg gacctctaca aatggcatct
      901 cgcgcatccg ggctccaact atcccaatct cgtctactgg ggaaaatacg tcgcgcacga
      961 caacaatcgc gacgccatgg gcgttacgct gaagctcacc gaaaacgtga tgaacactta
     1021 cgcgaagtgg aatccgcagg tgctgcacga tctgcacgaa tccgttccat acctctacga
     1081 caacacaatc ggcgacggtc cgtacaacgc gtggatcgat ccgattctcg ccgacgagtg
     1141 gaacctgatc agcggtcgca acgtcgccga aatgaccaag ttcgggatgc cgggcgtttt
     1201 cgcgcacggc gatttcgaca catggtcgcc gggctacctg atgtttatcg ccgccctgca
     1261 caacggcatc agccgcttgt atgaaacctt tggcaacggc ggtgccgaca cgctggagcg
     1321 caccctcgaa ccagaggaat acgcgcgcac ctggtacaag cagaatccgc cgctgcccaa
     1381 agtgctgtgg tcgcagcgcg acaacaacaa ctacgaagaa acgggactgc tggtttcgct
     1441 gcactacttc aacgaaaaca agcgcatgtt cctgaagaac ttttatctga aggcgaagag
     1501 gtcggtggag aagccgaagt cggaagggcc ggcggcgtat gtgttgccgg cggatgatcc
     1561 gcgcgctacc gtgcagctcg agttgctgaa ggtgatgcag aaacagcacg tggagatttc
     1621 gcggctgact tcggctacga caatagcgct gccgccgaag aaggagaaga aaccgaaggc
     1681 gggagaaaag aaagcggaga agaaggaaga aaagaaggac gaaaaaccgg agcccacgac
     1741 gaaaactttt ccggcgggga gctatgtgat tcgcatggac cagccgtact ctcgcatcgc
     1801 cgacgcgctg ctcgattatc agtattggag tccggaggat ccgcaaaaga ctccgtacga
     1861 cgatacggga tggacgtttg gcgagttgta tggagtgcag gtttcgcgcg tgaccgacgc
     1921 aaagattcta gaagcgccga tggaagccgt gaaggagttg agtgttgcgg caggagtgaa
     1981 aggcgagggc tcgattttcg cgattgaaaa tcacgccgag ccggcgcttg cctctctgcg
     2041 ctacaagctg aaagatgcgg acatgcaggc ggcggaagag gaatttgaag caaatgggaa
     2101 aaaattcgcg gcgggaaccg ttctggtgaa aggcgtggag cgcggccaat tcgactccgc
     2161 ggtgaaagag ctgggattgc agccggtgac gctcgcgtcg gcgcctagtg ttaagatgca
     2221 tccgctccgc gccgcgcaca tcttgctgct gcacacctgg ctgagcacgc agacggaggg
     2281 ctggtggcgg ctggcactgg acaaactcgg agtaccttac gactacgcca gcacgcagac
     2341 catcgctggc acgccggatt tacgcgaaaa atacgacgtg attttgtttc cgcccgtggg
     2401 cttcgccggc tcgttgaaca acatcgtgaa cgggctgccg accaactggg gcaatccgct
     2461 gccgtgggag aaaacggagc tgacgccgaa cctggtggga aaaaatgatt cgactcccga
     2521 catgcggccc ggcttgagtt gggcgggtgt agcgaagctg cagaaatttg tgaacgatgg
     2581 cggcgtgctg ctgaccgtga tggacacggc gcaatttgcg gcgcaggcgg gaatcaccac
     2641 gggtgtgacc agctctaact ctgacaagat gaaaattgtc ggatcagtgg tgggcacgcg
     2701 gctggtggat ggcgccagcc cgatcgcgta tggctacggc gaaaaggttg cggcgtattg
     2761 cgacaacgga ccggtgtttt cgctgaccag cattgcggga tcgcgtcggc ggcgccggct
     2821 gggtgcggag atgggcgagc ggccgacggg ccgcggcagc aagaatgatc cggactttgc
     2881 ggttggccgg gcaggcgtgg aagctcccga ggagacggaa agcgaactgt gggaagctcc
     2941 gccgatgacg gatgagctgc ggcacaacaa cccgcgagtg attccgccgg cgatgcgggc
     3001 gcgcgtggtg ttgcgctatg cggatgcgaa agacctgctg atttcgggat tggtgcaagg
     3061 cggcggggaa atcgcgcagc atccggcggt ggtggatgca ccgctcggca aaggacacgt
     3121 ggtgatgttt tcgatcaatc cggtgtggcg cggcgagacg caggggacct attcgctggt
     3181 gctgaacacg gtgatgaatt tcgacagcct gaatgccggg cggaaggacg cggagaagta
     3241 ggcgcaccgc agcggcgaaa ccaattcctg gagcggctaa tgccgtattt tcaggagacg
     3301 gataatgtct gggccaaaca aagctccgac cccccggttg ggcttgctat tgggcgaggt
     3361 tagagtacaa tccccgccgt catgagcaag aagagggcga agcgtagagc gaggagcaaa
     3421 ccacgccgtg ggagttcacg aaaatcctcg cggaattccg ggaagctgcc gaaggatgca
     3481 gcgatgctgc tggtgatttt gcggccgcgc gaaggccaag agctatttct ggaagcggaa
     3541 ttgcgagcgc taatggggcc gacgcgcaaa gaagagggct gcctgcgcta cgatttgtat
     3601 cgctcggcgg aagggactgc ggcgtacatg ctctacgaaa tctgggagtc gcgcgcgcat
     3661 cacacagcgc ataccaagac cgatcacttc acgcgctgga atgctcgcaa agattcgctg
     3721 ctggcttcgc gcgatgtggg cttctggaag cagattgcct gagggcaatc ttcaccaagg
     3781 tcgtctggaa caaactatga aaaaaaaatg gattgtggcc aatctcgcgc tcgccaccgc
     3841 gctggccatc gctcttgcgc catccggttt cgcgcaggag cccgagcatc aacatcacca
     3901 cgacggagcg gaaaatctgg gcaccgtaaa tttcccggtt tcgtgcgcgc ccgccgtgca
     3961 gcctgcattc aaccgtgcca tggccctgct ttattccttc gagtacgaat cttcgctgca
     4021 ggctttc
//
LOCUS       DASTPM010000414         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_677811, whole genome shotgun sequence.
ACCESSION   DASTPM010000414 DASTPM010000000
VERSION     DASTPM010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677811"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VFR42_07250"
     CDS             complement(<1..440)
                     /locus_tag="VFR42_07250"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU0368986.1"
                     /translation="MILLLDNYDSFTYNLAQYLGELGCEVEVHRNDKISVDEIARRKP
                     ERIVISPGPCTPQEAGISVELIQRLAGKFPILGVCLGHQAMGAAYGGKIVRAPKLFHG
                     KTSEIEHDGKGVFRELPQKFTATRYHSLIVEKKSLPKELSITAET"
     gene            complement(437..1927)
                     /gene="trpE"
                     /locus_tag="VFR42_07255"
     CDS             complement(437..1927)
                     /gene="trpE"
                     /locus_tag="VFR42_07255"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU0368987.1"
                     /translation="MIQPSFAEFSRLAKQGNLIPVYDVFSADLLTPVSAYLRIAQNTR
                     YSFLLESVEGGEKIARYTFAGAHPEEVFRYANGACVLETRDRLIWEEKDPITFLRAHM
                     ERYRPVRLAGLPPLIAGAIGYFSYDMVRLIEKLPQQLKDEIGLFDAVLMFYHGLIAFD
                     HVQHRLWIVRNVFTEGKGTLQQKYDAAVKEIEHTRKQLEQPVKAEHSSHKADNGSVAP
                     KVESNFKKPAYLAAIKKAKQYIRAGDIFQVVISQRFSAKTQAEPFQIYRELRALNPSP
                     YLFFLQLNDVAVVGSSPEMLVKVQGRDVFYRPIAGTRWRGKNEAEDLRLEKEMLADEK
                     ERAEHIMLVDLGRNDLGRVCEYGTVRVEKLMTVERYSHVMHLVSGLRGQLRPDVDCFD
                     ALMACFPAGTVSGAPKVRAMEIIEELEKTRRGIYAGGILYLDFAGNLDSCIALRTMVI
                     KNGVAHIQAGGGIVADSTPEGEYNESVNKSKALQRALEAAHNRNSK"
     gene            complement(2043..>2615)
                     /locus_tag="VFR42_07260"
     CDS             complement(2043..>2615)
                     /locus_tag="VFR42_07260"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368988.1"
                     /translation="AKNGKLHGSNTDYAGVLRALEGKLKLHGARALIFGAGGSARAAA
                     FALANAGAEVLISARRESAARELARACGAQTIARRLVASACFELIVNATPVGMHPHEG
                     VSPLSGKELNTPWVMDLIYRPLETELLCIAKKKGIGVISGLDMFLAQGFAQWELFMGQ
                     PAPESAMRRAVLKALRSEESSRCRDKKPLR"
BASE COUNT          542 a          792 c          703 g          578 t
ORIGIN      
        1 gtttcggcgg tgatggacaa ttccttcggc agagactttt tctcgacgat cagcgagtga
       61 tagcgggtag ctgtaaattt ctgcggcaat tcgcggaaca cgcctttgcc atcgtgttcg
      121 atctcgctgg tcttgccatg aaacaatttc ggcgcgcgca caattttgcc gccatacgcg
      181 gcgcccattg cctgatgccc gaggcaaacg cccagaattg ggaactttcc cgcgagacgc
      241 tgaatcaact ctaccgaaat acccgcttcc tgcggcgtgc aaggacccgg cgaaatcacg
      301 atgcgttccg gcttgcgccg cgcgatctcg tccacggaaa ttttgtcgtt gcgatgcact
      361 tccacttcgc aaccgagctc gcccaaatat tgggccaggt tgtaggtaaa ggagtcgtag
      421 ttgtcgagca gcagaatcat ttgctattcc gattgtgcgc ggcttccaac gcgcgttgca
      481 acgctttgga cttgttcacg gactcgttgt attcaccttc gggcgtggaa tcggccacga
      541 tgccgccgcc ggcctgaatg tgcgctacgc cgttcttgat gaccatggtg cgcagcgcga
      601 tacaggaatc cagattgccc gcgaaatcca gatacaagat tccgccagcg tagattccgc
      661 ggcgcgtttt ttcgagctct tcgataattt ccatggcgcg gacttttggc gcgccggaaa
      721 cagtgcctgc cgggaaacac gccatcaatg catcgaagca atctacgtcg ggccgcagct
      781 gaccgcgcag accagaaacc aggtgcatca cgtgcgaata acgctcgacg gtcatcaact
      841 tttcgactcg aaccgttccg tactcgcaga ctcgtcccaa gtcattgcgt cccaggtcca
      901 ccagcatgat gtgctcggcg cgttcttttt cgtcggccag catctctttt tcgaggcgca
      961 aatcttccgc ttcatttttg ccgcgccacc gggtgcccgc aatgggccgg taaaagacgt
     1021 ccctgccctg gactttcacc agcatttccg gcgagctgcc caccaccgcg acatcgttaa
     1081 gttgcagaaa gaaaagatac ggcgaaggat tcaacgcgcg cagctcgcga tagatttgga
     1141 acggctccgc ttgcgttttt gccgaaaagc gctggctgat cactacctgg aaaatatcgc
     1201 ccgcgcggat gtattgtttg gcctttttta tcgcggccag ataggcgggc tttttgaaat
     1261 tcgattcgac cttgggagcc acagaaccat tgtctgcttt gtgagaagag tgttctgctt
     1321 tgacaggctg ctccagctgt ttccgcgtgt gctcgatttc tttcaccgcg gcgtcgtact
     1381 tttgctgcag cgtacccttc ccttctgtaa agacattgcg cacgatccac aaccggtgct
     1441 gcacgtgatc gaacgcgatc agcccgtgat agaacatcag caccgcgtca aacagtccga
     1501 tttcgtcttt cagctgctgc ggcagctttt cgataagccg caccatgtcg tagctgaagt
     1561 atccgatggc gccggcgatc agcggcggca atccggccag ccgcaccggc cgatagcgct
     1621 ccatgtgcgc gcgcaagaag gtgatgggat ctttttcttc ccagatcagc cggtcgcgcg
     1681 tttccagcac gcacgcgcca ttcgcataac gaaacacttc ttccgggtgg gcgccggcaa
     1741 aggtatagcg agcaatcttt tcaccgcctt cgacagactc cagcaaaaaa gaataacggg
     1801 tgttttgcgc aatgcgcaga taggcgctga ccggcgtcag caaatccgcg gaaaaaacgt
     1861 cgtagacggg aatcaggtta ccctgtttgg caagtcggct aaattcggca aaactcggct
     1921 ggatcatcgc gcagtcatga ccgtttgatt tcgttgccgg gagaaccaca aacgccggaa
     1981 ggtggctggg tttccgcccg tcaattatcc acaaaaaggg ggcgccgcgc ttgaccggac
     2041 ggttaacgca gcggtttttt gtcgcggcag cgtgaagatt cctcggagcg caacgccttc
     2101 agaaccgcgc gacgcatcgc cgactctggc gccggctgcc ccatgaacag ttcccactgc
     2161 gcaaagccct gcgccagaaa catgtccaag ccggaaatca ccccgattcc tttcttcttc
     2221 gcgatacaca gcagttcggt ctcgagcgga cggtagatca gatccatcac ccacggcgta
     2281 tttaattctt tgcccgaaag aggcgaaacg ccttcgtgcg ggtgcattcc gaccggcgtg
     2341 gcattcacga tcagttcaaa acatgccgag gcgaccaggc ggcgcgcgat agtctgcgct
     2401 ccgcaagcgc gcgccaattc ccgtgctgca gattcgcggc gcgcgctaat cagtacttct
     2461 gcgcccgcat tcgctagcgc aaacgccgcg gcacgcgctg aaccgcccgc gccaaaaatc
     2521 agagctcgcg ccccatgcaa cttcagcttc ccttccagag cgcgcaatac gccggcgtaa
     2581 tccgtgtttg acccgtgcaa tttcccgttc ttggc
//
LOCUS       DASTPM010000415         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_994900, whole genome shotgun sequence.
ACCESSION   DASTPM010000415 DASTPM010000000
VERSION     DASTPM010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994900"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFR42_07265"
     CDS             complement(<1..197)
                     /locus_tag="VFR42_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368989.1"
                     /translation="MKRNQMFSALLTLLVLMLTAGNRVAAQNTATLSGVVKDQQGGSI
                     RAAKVTLTSKSTGAERTSVSN"
     gene            complement(577..3210)
                     /locus_tag="VFR42_07270"
     CDS             complement(577..3210)
                     /locus_tag="VFR42_07270"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0368990.1"
                     /translation="MNLFRKRAAPKPDEPSTSAPANAAPDRAPIPPVAEFPLRPSANS
                     GPGILEVHSALREVVILLHNNRLYHHAHPKYQQCLDHAYDNLQRLALSMNGLEIRVER
                     DAIVVPKLSEAPVPDPKNELRHLALDFQLAGIQTLVILRQFHVGELDTLAQLLRASLL
                     RSDESSRKNRSAWWTAKLLEFGVEGILVNAQTDRKVDTVLASLIAALVAFGGNTPNEN
                     ADAPITAPTADSLTAELRLIARLTPPLESARGLSPEEAARAIHAALAEASRDTVHLLL
                     SAISQHSPKEAEAPQPYLVRLSEQIIFEFINSEFSAGALLPQDVRKQLRSFGEVMVFS
                     GGYTGPHSSQHLSSLARVWADETHREKLLERFWMELPPREKSQTLRGPDAWCVPVPAI
                     RSALSQLAESGADAPRREARNILLNYTRRLEVPEPAVRRSVAVGLSELSAVVESLWPN
                     QLPEELSRCALKTLEQETAADTAALLSAFLEMLGRVAVTRGDFAGLENILLGLEKVPR
                     EPRFEHMHALAQRLIAQDRWHLLVDAALANRALEPTLPRLLVRDPERLLDRLTTLLSD
                     PRGGELVPAMARLLRTIGVPVLNLLETRLYEARKQRVSAAIKLLAAADPERLLRGLPR
                     ALASWEWNLQDLAVSELSRPANATSARSAAFVFATLLTEANALVVPMMIDHIGLAQET
                     SAIPELMQIASGTHESLKDQYVRIKAIEALARMRVSEAVDLLLQLSQRRDGLTYAEPS
                     GLRSVASDALALVENRPASAQVRAAYEYAPQAGGNFNVPRRYSRVPLESPLRAQIEGG
                     QRAMARVKTISLGGAYLESVKKLNVGDTIRLEVRSGLRKIHFTAVVRNAGPDGNGVEI
                     VHMQGDDRDKLRKLVQRGLKT"
     gene            complement(3451..>4176)
                     /locus_tag="VFR42_07275"
     CDS             complement(3451..>4176)
                     /locus_tag="VFR42_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU0368991.1"
                     /translation="VVDNLQYDDTAASITSKPAAAPGYAAIHIVTNVAGAIATVNSGS
                     SSAHQCETPCTFSDLDPGDYNLQVQKAGYQPVQTALQVKSGDALDQKVQLEPLAQGLF
                     IASHPAGADVFINGDKQSGQTPLTVPLAPGKYNLVLRLSGYDAYVSQIEVRSDGQSKV
                     NAEFHQKNGHIAWAQINSFPEGAEIWLDGSDTGQHAPARVEVPSGIHNIVLKLDGFQP
                     ARQVIQASEGGTIPVSKILSKVR"
BASE COUNT          830 a         1141 c         1334 g          871 t
ORIGIN      
        1 tcgtttgaga ctgacgttcg ctcagccccc gtgctcttgc tggtcagcgt gactttcgcc
       61 gcccggatgc tgcctccctg ctggtccttc actacgccgg acagggtcgc agtgttctgc
      121 gcagcgacac ggtttccagc ggtcagcatt aggacaagca aggtcaacaa cgcagaaaac
      181 atttggttgc gtttcatcac tgcctcccgg acaactggta cggcgcacag cattccccta
      241 gccgcatgtt gcacgccatg agaaaacgag ttgtgacttc cgaatgtccg ctacccccag
      301 cggccgtggc ttgtcacccc acgaataaac taccgtgccc acaaactacc gtaaatgatt
      361 ggctactcgg cttttacttg atagcaaaca gcattgcaac tctaaagttt gagctgccac
      421 tgtcctgcgt cctttcagtc acggccagac gcggatttta caagggcggc gcagcgggag
      481 aacttcccgt acaagcggaa agcgagccgg cttgccgcat cggctcgatg attcattacg
      541 gttaatgaaa cggatttatg gctggatctg cgaaattcag gttttcaagc cgcgctgcac
      601 gagtttgcgc agcttgtcgc ggtcgtcgcc ctgcatatgg acgatttcga cgccgttgcc
      661 atccggtccg gcgttgcgta ccaccgcggt aaagtgaatc ttgcgcaggc cggaacggac
      721 ttcgaggcgg atggtatcgc cgacgttcaa ttttttcacc gactcgagat acgcgccgcc
      781 gaggctgatg gttttgacgc gggccatggc ccgctggccg ccttcaatct gggcgcgcaa
      841 gggcgattcc agcggcacgc gggaatatct gcgtgggaca ttgaaatttc cgccggcttg
      901 cggagcgtac tcgtatgcag cgcgcacttg agcggacgcg gggcgattct ccaccagcgc
      961 cagcgcatcg cttgcgacgg agcgcaagcc ggagggttcg gcataggtca gaccgtcgcg
     1021 cctctggctc agctgaagca gcagatcgac cgcttccgaa acgcgcatgc gcgccagcgc
     1081 ttcgatggct ttaatacgca catactgatc ttttaacgat tcgtgggttc cactggcgat
     1141 ttgcatcagt tccggaatgg cagaagtttc ctgggccaga ccgatgtggt cgatcatcat
     1201 cggcacgacc agcgcatttg cttccgtgag caaggtcgca aacacaaacg ccgcactgcg
     1261 cgccgaggtt gcgttcgccg gccgcgaaag ttcgcttacg gcgagatcct gcaaattcca
     1321 ttcccaactg gccagggcgc gcggcaatcc gcgcagcagg cgctcgggat cggcggcggc
     1381 cagcaacttg atggcggcgc tcacccgctg cttgcgcgct tcgtataaac gcgtctcgag
     1441 caagttcaaa accggcacgc cgatggtgcg tagcagccgc gccatagccg gaacaagttc
     1501 gccgccgcgc ggatcactca acaaagtggt caggcggtcc agcaggcgtt cgggatcgcg
     1561 caccagcaaa cgcggcagag tcggttcgag agcgcgattt gccagcgcgg catccaccag
     1621 taggtgccag cgatcttgcg cgatcaggcg ctgtgccagt gcgtgcatgt gttcaaagcg
     1681 cggctcgcgc ggaacttttt cgaggcccag caggatattt tccaggccgg caaaatcgcc
     1741 gcgggtgacc gcgacgcgac ccagcatttc aagaaaagcg gaaagcaggg cggccgtgtc
     1801 agcggcggtc tcctgttcca aagtcttgag cgcgcagcgg cttagttctt ccggcagctg
     1861 attaggccac agtgattcga cgactgcgga aagttcgcta agtccaacgg cgacgctgcg
     1921 gcgcaccgcc ggctccggca cctctagacg ccgcgtgtag ttcagcagaa tgttgcgggc
     1981 ttcgcgcctg ggcgcgtcag cgccggattc ggcgagttgt gagagcgcag agcgaatggc
     2041 cggaaccgga acgcaccagg catcggggcc gcgcagcgtt tgcgattttt cgcgcggcgg
     2101 cagttccatc cagaatcgct ccagcaattt ttcgcgatgc gtttcgtcgg cccagacgcg
     2161 ggccagcgaa gaaagatgct gcgaagagtg cgggccggta tagccgccgg agaaaaccat
     2221 cacttcgccg aaggatcgaa gctgtttgcg cacgtcctgt ggcagcaaag cgccggcgga
     2281 gaattctgaa ttaataaatt caaaaatgat ttgctcggaa agtcgtacga ggtagggctg
     2341 tggagcttcc gcttctttgg gagagtgctg ggaaatcgcg ctgagcaaga ggtgaacggt
     2401 atcgcggctg gcctcggcca gggcggcgtg aatggcgcgg gccgcttctt cgggagacag
     2461 gccgcgcgcg ctttcaagcg gaggggtcag ccgagcaatc aggcgaagct ctgctgtgag
     2521 gctgtccgcg gtcggcgccg tgattggcgc gtccgcattt tcgttgggag tgttccctcc
     2581 aaacgccacc agtgcggcga tcaaactggc aagcacagta tctacttttc ggtcggtctg
     2641 ggcgttcacg aggatgcctt ctactccgaa ttccagcagc ttggcggtcc accacgcgct
     2701 gcgatttttc ctggacgact catctgatct tagcaatgaa gcgcgcagga gttgtgccag
     2761 ggtgtccagt tcgccgacat gaaattgccg aaggatcact aatgtctgaa ttcccgccag
     2821 ctggaagtcc agggcgagat ggcgcagttc gtttttggga tcagggacgg gagcttcgct
     2881 cagtttgggc accacaatcg cgtcgcgttc gactcgaatt tccaggccgt tcatgctcag
     2941 cgccaggcgc tgcaagttgt cgtaggcgtg gtcgaggcat tgctggtatt tgggatgagc
     3001 atgatggtag agccgattgt tgtggagcag gatgactact tcgcgcaagg cgctatgaac
     3061 ttccaggatg ccggggccgg aattcgcgga gggccgaaga ggaaattcag cgaccggcgg
     3121 tatcggagct cgatcgggcg ccgcgtttgc cggcgcggac gtggacggtt cgtcgggttt
     3181 gggtgcagcg cgtttgcgaa atagattcat caagaatttg accgcccaac tctgtctaag
     3241 ggccccaaaa tccgcgcata gaaacccgcc aaaggcaggg agcggcgtag atagaagggc
     3301 aatcggagtg ccggggctag agggttgcaa agggcacaaa acactaggtt tagttcttat
     3361 agtactaaaa cttgcattcg tcaatcgtaa cgttttgtta cgtaacgcgc catctgttgg
     3421 tcaggagatc cggcaagcgg gaatcgtcgt tcaacgaact ttgcttagga ttttggaaac
     3481 gggaatggtg ccgccttcgc tggcttggat gacttggcgg gcgggctgga agccgtccag
     3541 tttgaggacg atgttgtgga tgccggaggg gacttcgaca cgggcgggag cgtgctgccc
     3601 ggtatcgctg ccatcgagcc aaatttcagc gccctctgga aaagaattga tctgggccca
     3661 ggcgatgtga ccgtttttct gatggaactc ggcgttgacc ttggattggc cgtcggagcg
     3721 gacttcgatc tggctgacgt aggcgtcata gccggagaga cgcaggacga ggttgtattt
     3781 gcccggtgcc agcggcacgg tgagaggcgt ctggccggat tgtttgtcgc cgttgataaa
     3841 gacgtcggct ccggcagggt gcgaagcgat gaataggcct tgcgcgagag gctcgagctg
     3901 cactttttga tcgagtgcat cgccagactt gacctgcagc gcggtttgca ctggctggta
     3961 accagccttt tgaacctgta agttgtaatc gccgggatcg aggtcggaaa aggtgcacgg
     4021 cgtttcgcat tggtgagcgc ttgaagagcc agagttgaca gtagcgattg cgccggcaac
     4081 gttggtgact atgtggatgg cagcgtagcc gggtgctgcc gcgggcttgg aagttattga
     4141 cgcggcggtg tcgtcatact gcaggttgtc aacaac
//
LOCUS       DASTPM010000416         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_591208, whole genome shotgun sequence.
ACCESSION   DASTPM010000416 DASTPM010000000
VERSION     DASTPM010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591208"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFR42_07280"
     CDS             complement(<1..322)
                     /locus_tag="VFR42_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368992.1"
                     /translation="MRYLKYAALFGLLAFGFAGSAKAQVRVAVGVGPVYGTVYGSAPV
                     CAYGYYDYYPYACAPYGYYGPDWFVNGIFIGAGPWYHGYYGRGFYPRGYYGRGYYGRG
                     YYGRP"
     gene            689..1717
                     /locus_tag="VFR42_07285"
     CDS             689..1717
                     /locus_tag="VFR42_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0368993.1"
                     /translation="MASGISRYSPDAKGLIVARSAICSYYREKHRVFLSSDDLLLTSG
                     TSEAYSFLFRLLCNPGDEILIPAPGYPLFDFLADLCDVKLVRLPLIYDHGWQIDLTAL
                     TRAATERTRALIVVHPNNPTGHYVSARERDRLAAFCSDRGIALIADEVFLDFSLRDRE
                     CAKSFADSRNVLTFTLSGLSKICGMPQMKLAWIVVSGPEVQKSEAMARLEVIADAYLS
                     VGTPVQLAAPALLQMRGEFHSQLIERVRANLAELDRQLAWQSNCTRLECEAGWTAVLR
                     IPALESDEELAIALLNESGVYVHPGHFYDFARPGHMVISLIVPPEDFAEGTRRVLAHF
                     AKFATDRW"
     gene            complement(1743..>2317)
                     /locus_tag="VFR42_07290"
     CDS             complement(1743..>2317)
                     /locus_tag="VFR42_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0368994.1"
                     /translation="ATTALDLVKGDQDVIVLRTFSKIYGMAGLRCGLAIGRPDLLEKI
                     SGFSGWNSLPVTAVAAAITSLGDASLVPERKQVNAAVRADTLAFLDRSGYRYTPSASN
                     CFMLDTKRPTKEIIDAMAARNVFIGRAWPVWPTHVRITVGTKAEMEKFQAAFREVMQA
                     KPARIASPRSRDLWANLDGIRVPQKGSGGV"
BASE COUNT          510 a          677 c          639 g          491 t
ORIGIN      
        1 ccggtcgccc ataatagcct cgcccatagt agccgcgacc gtagtaaccg cgcgggtaga
       61 atccgcgccc gtagtagccg tgataccagg gaccggcgcc aatgaagatg ccattcacaa
      121 accaatcagg cccgtaatag ccgtagggag cgcacgcgta aggataataa tcgtagtagc
      181 cgtaagcgca taccggagcc gacccgtata ccgtgccgta gaccggcccg actcccaccg
      241 ctacgcgaac ttgcgctttg gcgcttcctg caaaaccaaa agccaaaaga ccaaacaacg
      301 cagcatattt tagatatcgc attgtgttta cctgttccgg ctcgtttgcc agacagaccg
      361 attagtttcc cggctttctt cgcctggggg aagccactta caatagctta aacccggccc
      421 tgggaagaaa gttgccgttt ccaggaagtc tcgcaagtac aagaaaataa gctagatagc
      481 taaacttcat ttcttggata taatgctcgc tcattttact cttactccta tgttttcaga
      541 gcggactgcc tggaatcgag ccgaaaacga gctgacccgc atgctggata agctgcgggc
      601 aaccggaacg cggatcttag atctcacacg ctccaacccc accgaatgcg gctttgcccg
      661 ggatgagacc aagatcgcga gtgccttttt ggcaagcgga atcagccggt attcgcccga
      721 cgccaaaggt ttaatcgtcg cgcgtagcgc gatttgtagc tactaccggg aaaaacaccg
      781 ggtctttctg agcagcgacg atttgctgct gacttcggga acgagcgaag cctattcgtt
      841 cttgttccgc ctgctgtgca atccgggcga cgagatactc atccccgccc caggatatcc
      901 tttgttcgat tttctggccg atctttgcga cgtcaagctg gttcgccttc cgctcattta
      961 cgaccacgga tggcagatcg atcttacggc gctgacccga gcggcaacgg agcgcacacg
     1021 agcgctgatc gtcgtccacc cgaataaccc gacgggccac tacgtaagtg ctcgcgaacg
     1081 agatcgcttg gctgcatttt gctccgaccg cggaattgct ctgatcgccg acgaagtttt
     1141 cctggatttt tcgctgcgcg atcgagaatg cgccaaaagc ttcgcggatt ctagaaatgt
     1201 actcacgttt acgctgagcg gcctatccaa gatctgtggc atgccgcaga tgaagctggc
     1261 ctggatcgta gtgagcggtc cagaagtgca aaaaagcgag gccatggcgc gactggaagt
     1321 catcgccgac gcctatctct ccgtcggtac gcccgtgcaa ctggcggcac cggctctgct
     1381 gcaaatgcgc ggagagtttc acagtcaact catcgagcga gttcgcgcga atctggcgga
     1441 gctggatcgc caactagcct ggcaaagcaa ttgcacacgg ctggaatgcg aagccgggtg
     1501 gacggctgtc ttgcgcattc cagctttgga aagcgacgaa gaacttgcga ttgctctgct
     1561 aaacgagagc ggcgtttacg tccatcccgg ccatttttat gattttgcgc ggccggggca
     1621 catggtgatc agcctaatcg tgcctccgga ggactttgct gagggtacgc gccgcgtcct
     1681 ggcgcacttc gccaaattcg cgacggatcg ttggtgagca tcgcctcgta agttcggttc
     1741 agctaaacgc ccccactccc tttttgagga acgcgaatgc catcaaggtt ggcccagaga
     1801 tcacggctgc gcggagaagc gatgcgcgcc ggtttggcct gcatcacctc acgaaacgcc
     1861 gcttgaaatt tctccatctc tgcttttgtg cccacggtaa tgcgaacgtg cgtgggccac
     1921 actggccagg cgcgcccgat aaaaacgttg cgggcggcca tcgcatcgat gatttctttc
     1981 gtaggacgct tggtgtccaa catgaagcaa ttcgacgccg atggcgtgta gcggtagccg
     2041 ctgcgatcga ggaaagccaa ggtatcggca cgcaccgcag cattaacttg tttgcgctcc
     2101 ggcacgagtg aagcatcgcc cagactggta atggctgcgg caacggcggt gaccggcagg
     2161 ctgttccatc cgctgaaccc agagattttc tccagcaaat ccgggcggcc aatcgccagc
     2221 ccgcagcgca gcccagccat gccgtaaatt ttcgagaacg tacgcagaac aatcacgtcc
     2281 tggtcgcctt tgaccaggtc gagtgcggtc gtcgcgt
//
LOCUS       DASTPM010000417         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_966062, whole genome shotgun sequence.
ACCESSION   DASTPM010000417 DASTPM010000000
VERSION     DASTPM010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966062"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFR42_07295"
     CDS             complement(<1..900)
                     /locus_tag="VFR42_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368995.1"
                     /translation="MVFPGVRKVLLIAAGSFLAILVLFFAIAWSQKRAAELRTRDWIV
                     QLLQEKFQSNVELADFHVRVFPRMTVSGEGLSLHYWATPEAPALIRIEKFSFDLGLLG
                     IFRAPHRIQRIHLKRMVISIPPREMRKAAAIRPVDKLNAPEVVVGTIEVEDMDLLILS
                     SKPGVDPLDWEIHDLKLHEVGADKAFAFSGTLTNAKPKGEIATSGKFGPWDGDDPGST
                     PVSGAYAFDNADLGPFPGIAGILSSTGKYAGVLNRLEVNGETDTPDFSLDKAGKPIPL
                     HTDYSATVDGTNGDTRLHPVHAIL"
     gene            complement(933..1466)
                     /locus_tag="VFR42_07300"
     CDS             complement(933..1466)
                     /locus_tag="VFR42_07300"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEU0368996.1"
                     /translation="MMRRISFVSAVLLSLACAMAAQIPSARDVVKPTAYASFEPAARG
                     KQLEIAVVMKIREGFHVNARETTFDYLIPTDLKTEPAPGFKIGAVKYPKGTLHKFSFA
                     KDQALNVYADTVVLFVPVAVDANAPLGEQHVALKLRYQACNNEVCLPPVTLPVDAAIH
                     VSSGESRPSHPELFAKP"
     gene            complement(1463..1801)
                     /gene="cyaY"
                     /locus_tag="VFR42_07305"
     CDS             complement(1463..1801)
                     /gene="cyaY"
                     /locus_tag="VFR42_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEU0368997.1"
                     /translation="MPMLEERDFQKKCEAALEELKLRLLDAGDQHGFDVEGESGKLEV
                     VFEEPAPAKFVVSPNTPVREIWISALSTSFKLGWKEEKSAFVLEKTGEDLFAVLSRVI
                     SQQLGEEVKV"
     gene            complement(1806..>2036)
                     /locus_tag="VFR42_07310"
     CDS             complement(1806..>2036)
                     /locus_tag="VFR42_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0368998.1"
                     /translation="KSVVAPFVTKERYDVNGQKLPMNYPIVVGNEKVAEKFGGLLGYP
                     TSILISRDGRILKRTTGLIDYDATTKLIEGAL"
BASE COUNT          436 a          596 c          552 g          452 t
ORIGIN      
        1 gaggatggcg tggacgggat gcaatcgagt gtccccgttc gttccgtcga ccgtcgctga
       61 atagtcagtg tgcagcggaa tcggctttcc tgccttgtcg agcgaaaagt ccggagtatc
      121 ggtttctcca ttcacttcca ggcgattaag cacgcccgca tacttccccg tggaagaaag
      181 gatgccggcg attccgggaa acggcccgag atcggcgttg tcaaacgcat acgctccgga
      241 caccggagta gagccggggt cgtcgccgtc ccacgggcca aattttccgg aagtggcgat
      301 ttcgcctttg ggcttggcgt tcgtcagtgt ccctgaaaaa gcaaacgctt tgtccgcgcc
      361 gacttcatgg agcttcaaat cgtgaatttc ccaatcgagc gggtcgacgc ccggcttgct
      421 ggaaagaatc aacaggtcca tgtcttcgac ttcgatggtg cccacgacga cttcgggagc
      481 attgagtttg tctaccgggc gtatcgccgc cgccttacgc atttcgcgcg gcggaatgga
      541 aatgaccatt cgcttcaagt ggatgcgctg gatgcggtgc ggtgcgcgaa aaatgcccag
      601 cagcccgaga tcgaacgaga atttttcgat gcggatcaac gcgggcgctt caggcgtggc
      661 ccaataatgc agggacaatc cttcgccaga gaccgtcatt cgcggaaaca cgcgcacgtg
      721 aaaatccgct agttccacat tgctttggaa cttttcctgc agcagctgca cgatccagtc
      781 gcgcgtgcgc agttcggccg ctcgcttttg cgaccacgct atcgcaaaga aaagtacaag
      841 gatagccagg aacgaaccgg ccgcaatcag cagaactttt cgaacacctg ggaagaccat
      901 gaaggaagtt tcgcaaacag atcgcagcca cgctacggtt ttgcgaacaa ttcggggtgg
      961 ctagggcgag attctccgga agatacatgg atcgcggcat ccaccggcag cgttacgggc
     1021 ggcaggcaca cttcgttgtt gcaagcctga tagcgcagct tcagcgcgac gtgctgttcc
     1081 ccgagcggag cgttcgcatc aaccgcgacc ggcacaaaca aaaccaccgt gtccgcgtag
     1141 acgttcagcg cctgatcctt ggcaaaactg aatttatgca gcgtgccttt gggatatttc
     1201 accgccccaa ttttaaaacc tggcgccggt tccgttttca ggtcggtcgg gatcagatag
     1261 tcgaacgtgg tttcgcgcgc gttgacgtga aagccttcgc ggatcttcat aaccaccgcg
     1321 atttccagtt gtttgccgcg cgccgccggc tcgaacgatg cgtacgccgt gggcttgact
     1381 acgtcgcggg ccgatggaat ctgtgccgcc atcgcgcagg cgagcgacag aagaaccgcc
     1441 gagacaaaag aaattctgcg catcatactt tcacttcttc tcccagctgc tgggaaatca
     1501 cgcggctcaa tacggcgaac aggtcttcgc cagtcttttc gagcacgaat gcgctctttt
     1561 cctctttcca gccgagctta aaactggtcg aaagcgccga aatccagatt tcacgcacgg
     1621 gcgtgttggg cgaaaccaca aatttggcgg gagccggttc ttcgaacact acttccagct
     1681 tgccgctctc gccctccacg tcgaagccgt gctggtcgcc tgcgtccagc agccgcagct
     1741 tcaattcctc cagcgccgcc tcgcactttt tctgaaaatc gcgctcttcc agcatcggca
     1801 tcgaactaca gcgctccctc gatcagtttt gtcgtcgcgt cgtaatcgat caagccggta
     1861 gtgcgtttca aaattcttcc atcgcgcgaa atcagaatgc tggtcggata acccagcaaa
     1921 ccgccgaatt tttcagccac tttttcattt ccaacgacaa tcgggtagtt catcgggagt
     1981 ttctgcccgt ttacgtcata tcgttctttc gtgacaaacg gcgccacgac tgattt
//
LOCUS       DASTPM010000418         5175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_158584, whole genome shotgun sequence.
ACCESSION   DASTPM010000418 DASTPM010000000
VERSION     DASTPM010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158584"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /gene="mutL"
                     /locus_tag="VFR42_07315"
     CDS             <1..1755
                     /gene="mutL"
                     /locus_tag="VFR42_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU0368999.1"
                     /translation="ATLGFRGEALPTIASVSRLLLESRDAAEAEGTRVEFANGKLVSV
                     KPAGLPAGTTLCVTDLFYSVPARRKFLKSDTTELGHIASLVTHYALANPDKQIVLTTP
                     TQTIIDCPPAEKLADRVYQLFGRQALDELVEIPTFSSPFRAAITEPPLDDGEEKATLT
                     VTGFTSRPEIQRTNKSGIYIFVNKRLVRDKLVLHAIHEAYRNIHPHAISPATLLFLEM
                     PYDEVDVNVHPKKIEVRFRRSQFVHDAVRDAIRQALMQARPIASFAVAAGTAPASQAN
                     AAAWNSQGSSLHPVTPVPAQSGIPRAMIPPMGVIGLGSGVGSDFSGGVGSGVGSDGGF
                     DLSSAPLQPVEQRFAFEPGWSFASGIPSAARTPATSAWMGNLAGANGDAPASLPHPDQ
                     IADLKPLGQVSSSFIIAVNGQGLWIVDQHVAHERVLFEQHLEARRAGKVESQRMLMPM
                     IVELSPRQIVIFEKIAEELVANGFEVEPMGPKSFAIQAIPAGIVTSDSEKLLTEILDG
                     IERENAAISIETLQAKIAASTACHAAIKVNMPLDQTKMEWLLAALAKTDCPMSCPHGR
                     PVVLRYSVKEIERAFQRI"
     gene            1902..2666
                     /locus_tag="VFR42_07320"
     CDS             1902..2666
                     /locus_tag="VFR42_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEU0369000.1"
                     /translation="MTDSQKTAVVFGLANKRSIAWAIAQKLQEAGWRLAITYQNERLE
                     QEAKDLISDLPGAAGFMCDVSSDEQIAKLFEVLKEKYGTLQGIVHSVAFAPAEELKGE
                     FVHTSREGFRIAHDISVYSLVAVSRAAAPLMTEGGGIVTLSYFGAEKVVPRYNVMGVA
                     KAALEASVRYLASDLGPKGIRVNAISAGPIKTLAARGISNLGDMLKAHADRSPLRRNV
                     DPAEVGGTAAFLLSSAGSGITGETIYVDCGYNIMGF"
     gene            2697..3419
                     /gene="cmk"
                     /locus_tag="VFR42_07325"
     CDS             2697..3419
                     /gene="cmk"
                     /locus_tag="VFR42_07325"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU0369001.1"
                     /translation="MKKLTIAIDGPAGSGKSSVARIVAQRLGYLYLDSGAMYRALALK
                     ALQNNGQALTSEHELEQLARDTQIELQPVPATLQDPTVHNRVLLDGDDVTQSIRTREV
                     AQAASRVATIPGVRAVLVAAQQRAGANGGVVMEGRDIGTVVFPNAELKIFLEAAPEAR
                     AERRWKEHREKGERLELAQVIDEVRERDKRDTERKVSPLVRAADAVLVDNTAMDVEET
                     ARLIIMLAHERQKGVSLTRSLE"
     gene            3491..4576
                     /locus_tag="VFR42_07330"
     CDS             3491..4576
                     /locus_tag="VFR42_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU0369002.1"
                     /translation="MHSQMTYFLRDILRQPKELRWTLEHLKGAGAESITAAANAISKA
                     KHVYLTGIGSSWHAALNVAPMFQEAARPVYLVEASELLEFGTFAAESAVIVISRSGRS
                     IEIVRLAEKAIRAGAALIGITNAPEGTLAKETEFPIVVPIALDHAISVNTYSTLAAAA
                     GVLVKTVIGGFGAEDAGELSSAIDEAGHAVPRWQKQIAESKWFAPGRTTYFLARGSSF
                     GTAQEARLLWEEGVKAPATAMGTGVFRHGPQEMVREGMRFGIWLDAARMREEDLATAR
                     DLQKLGAAVMLIGQGVPADAVDLVLGLPKIAGEWQFLVDCMPAQLAAEQLARISGVDS
                     DTFRLCSFVVEDEAGLLGGATGDGARS"
     gene            4573..>5175
                     /locus_tag="VFR42_07335"
     CDS             4573..>5175
                     /locus_tag="VFR42_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0369003.1"
                     /translation="MIGAVDIGGTKIAVGIVDGNGRVVSRVEAPTEAERGYAVGLQKI
                     TKMLRKIWGGTGAKIRGIGIGSTGMLDPICGEWTDVDFLPGWEGRNPVEDLSREFGVS
                     AAMENDGDAGALAETYWGAGKGKSRVVYVTVGTGIGGGVILDGHLYRGVAGSHPEIGH
                     HVMEVSGPECTCGFRGCWEALAAGSSMEKWYAENRPAAERY"
BASE COUNT         1109 a         1417 c         1656 g          993 t
ORIGIN      
        1 gccacgctcg ggtttcgcgg cgaagcgctt cccaccatcg cttccgtttc gcggctgctg
       61 ctggaatcgc gcgacgccgc cgaagccgaa ggcacgcgcg ttgaattcgc caacggcaag
      121 ctggtctccg taaaaccggc cggtcttccc gcaggcacca cgctctgcgt caccgatctt
      181 ttttactccg tccccgcccg ccgcaaattt ctgaaatcgg acaccacaga actcggccac
      241 atcgcctcgc tcgtcacgca ttacgcgctc gccaatcccg acaaacaaat tgtccttacc
      301 acgcccacgc aaaccatcat cgactgcccg cctgccgaaa aacttgctga ccgcgtttac
      361 cagctcttcg gacgccaggc actcgacgaa cttgtcgaga ttcccacgtt ctcctcgcca
      421 tttcgcgccg ccatcaccga gcccccgctc gatgacggag aagaaaaggc caccctcacc
      481 gtcaccggct ttacctcacg ccccgaaatt cagcgcacca acaaaagcgg catctacatt
      541 ttcgtgaaca agcggctcgt gcgcgacaaa ctagttctgc atgctatcca cgaggcctat
      601 cgcaacatcc atccgcacgc gatctctccg gcgacattgc ttttcctcga aatgccgtat
      661 gacgaagtgg atgtgaatgt tcaccccaaa aaaattgaag ttcgcttccg ccgctcgcag
      721 tttgtgcacg acgcagtgcg cgatgcgatc cgccaggccc tgatgcaagc gcggcctatc
      781 gccagcttcg cggtcgccgc aggcactgcg cccgcatcgc aagccaatgc cgctgcctgg
      841 aattcgcaag gcagctcgct gcatccggta acgccagttc ctgcgcagtc cggcattcca
      901 cgcgccatga ttccaccgat gggagtgata ggacttggtt caggagtcgg ctcggatttc
      961 agcggaggcg tcggcagcgg agtcggctcc gacggcggct tcgatctctc ttccgcgccg
     1021 ctgcagccgg tggagcaacg ctttgctttt gagccgggct ggtcgttcgc gagtggcatt
     1081 ccatccgccg cgcgcactcc agctacgtcc gcttggatgg gcaatctcgc cggcgccaac
     1141 ggtgatgctc ccgcctcact cccacatccc gatcagatcg ctgatttgaa accgctcggt
     1201 caggtcagct ccagcttcat catcgccgtg aacggccagg gattatggat cgtcgaccag
     1261 cacgtcgccc acgaacgggt tttatttgag cagcacctcg aagcgcgccg cgccggaaaa
     1321 gttgaatcgc agcggatgct gatgccgatg attgtcgaac tgtcaccgcg tcagatcgta
     1381 atcttcgaga aaatcgccga agaactcgtg gccaacggtt ttgaagtcga gccaatgggg
     1441 ccaaagagtt ttgccattca agcgattcct gcaggaattg tcacgagcga ctccgaaaaa
     1501 ctgctaaccg agattctcga tggcatcgag cgcgaaaacg ccgccatttc catcgaaacg
     1561 ctgcaggcca aaatcgccgc ctcaacagcg tgtcacgccg ccatcaaggt gaatatgccg
     1621 ctcgaccaga ccaaaatgga gtggctcctc gccgccctcg ccaaaaccga ctgccccatg
     1681 agctgccctc acggccgccc cgtcgtcctc cgctattcgg taaaagaaat cgagcgcgct
     1741 tttcagagga tttagtcgta gtgcggccct ttagggcggc aagaagtggc gggtggtgag
     1801 tcaaggctgc taaaatcaca acgctttcag gggctcaatt tcttttccgt tctttcggaa
     1861 aactgacgac ggaaagcaga aaaccgaaaa ttgaggacta cgtgaccgat tcgcaaaaga
     1921 cagcagtggt tttcggcctg gccaataagc gcagcattgc atgggccatc gcgcagaaat
     1981 tgcaggaagc gggctggcga ctcgccatta cctaccagaa cgaacgcctc gagcaggaag
     2041 cgaaagacct gatctccgat cttcccggag cggcgggatt catgtgcgat gtgtcttccg
     2101 acgagcagat cgccaagctg ttcgaggtgc tcaaagaaaa atacggaacc ttacaaggta
     2161 ttgtgcacag cgtggcattc gctccggcgg aagaattgaa gggcgagttc gtgcacactt
     2221 cgcgcgaggg ttttcgtatc gcgcacgaca tcagtgtgta ttcgctggtt gccgtttcgc
     2281 gcgccgcggc tccgctgatg accgaaggcg gcggcatcgt tacactttct tatttcggtg
     2341 ccgaaaaagt tgtaccgcgt tacaacgtga tgggagtcgc caaagctgct ctggaagcga
     2401 gcgtgcgcta tcttgcaagt gacttgggac cgaagggtat tcgggtcaac gcgatttcgg
     2461 ccggcccgat caagacgctc gcggcgcgcg ggatttcgaa ccttggcgac atgctcaaag
     2521 ctcacgccga tcgctcgccg ctgcggcgca acgtggatcc cgcggaagtt ggcggcactg
     2581 cggcattcct gctttccagc gcaggctccg gcatcaccgg cgaaaccatt tatgtggatt
     2641 gcggctacaa cattatgggg ttctagtcgc agcagccgaa aattgtcgaa tcctgcatga
     2701 aaaaactgac gatcgcgatt gacggacctg ctggctcggg gaaaagcagc gtggcgcgaa
     2761 tcgtcgcgca gcggctgggc tatttgtatc tggatagcgg cgcaatgtac cgtgcgcttg
     2821 cgctgaaggc cttgcaaaac aacggtcaag cgctgacttc cgaacacgaa ctcgaacagc
     2881 ttgcccgcga cacgcaaatc gaactgcagc ccgtgccagc gactttgcag gatcctacgg
     2941 tacacaatcg cgtgctactc gacggcgacg acgtgacgca gtccatccga accagagaag
     3001 tggcgcaagc cgcttcgcgg gttgcaacga ttcccggtgt gcgtgcggtg ctcgtcgcag
     3061 cgcagcagcg ggcgggggcg aatggcggcg tggtgatgga ggggcgcgac atcggcaccg
     3121 tggttttccc aaatgccgag ctgaagattt ttctggaagc ggccccggaa gcgcgcgcgg
     3181 aacgccgctg gaaagaacat cgggagaaag gcgaaaggct ggagctcgcg caagtgattg
     3241 atgaagttcg cgaacgggac aaacgcgaca ccgagcgcaa ggtctctccg ctggttcgcg
     3301 cggcggacgc cgtgctggtg gacaacaccg cgatggacgt ggaagaaacg gcgcggctaa
     3361 ttattatgct ggcgcacgaa cggcaaaagg gagtctccct gaccagaagc ctggagtgac
     3421 tgtatttgta agagtcctgt cggccgcggt gcgccacagg atagcgccaa tatagaaagc
     3481 aggaagggcc atgcactcgc aaatgacata tttcttgcgc gacattttgc ggcagccgaa
     3541 agagttgcgt tggacgctcg aacatttgaa gggcgccgga gcggagtcga tcacggcggc
     3601 ggcgaatgct attagcaaag cgaagcatgt gtatttgacg ggaatcggaa gcagctggca
     3661 tgctgcgctg aatgttgcgc cgatgtttca ggaagcggcg cggccggtgt atctggtcga
     3721 agcgtcggag ctgctggagt ttggcacctt tgccgcggag agtgcggtga tcgtgatttc
     3781 gcgcagcggg cgcagcattg aaattgtgcg gcttgcggaa aaagcaatcc gcgcgggcgc
     3841 ggcgttaatt gggataacga acgcgccgga aggaacgctg gcgaaagaaa ctgagtttcc
     3901 gatcgtggtg ccgattgcac tggaccacgc aatttccgtg aacacctaca gcacgctggc
     3961 ggcggcggct ggagttctgg tcaagacagt gattggcgga ttcggcgcag aggacgcggg
     4021 cgaacttagt agcgccatag atgaagcagg gcacgcggtt ccgcgatggc agaagcaaat
     4081 tgccgaatcg aaatggtttg ctcctgggcg gacgacgtat tttctggcgc ggggttcgag
     4141 ttttgggacg gcgcaagagg cgcgactact gtgggaagag ggagtgaaag cgccggcgac
     4201 ggccatggga acgggagtgt ttcgacacgg gccgcaggag atggtgcgcg aaggcatgcg
     4261 gtttggcatt tggctcgatg cggcgcgaat gcgcgaagag gatttggcca cggcgcgcga
     4321 tctgcagaag ctgggcgcag cggtgatgct gatcgggcag ggagttccgg cggatgccgt
     4381 ggatcttgtt ctcgggttgc cgaagattgc gggtgagtgg caattcctgg tggactgcat
     4441 gccggcgcag ctcgcggcgg aacaactggc gcgaatctct ggtgtggaca gcgatacgtt
     4501 tcgtctgtgt tcgtttgtgg ttgaggatga agcgggattg ctgggcgggg cgacgggcga
     4561 cggagcgaga agttgattgg agcggtggac atcggcggta cgaagattgc cgtcggcatc
     4621 gtggacggga acgggcgggt ggtgagtcgc gtggaagctc cgacggaagc agagcgggga
     4681 tacgcggtcg gtctgcagaa aattacgaaa atgttgagaa agatttgggg cgggactgga
     4741 gccaagattc gcggaatcgg gatcgggtcg acgggaatgc tggatccgat ttgcggagag
     4801 tggaccgacg tggattttct gccgggatgg gaaggaagaa atccggtgga agatttgtcg
     4861 cgcgaatttg gcgtgagcgc ggcgatggaa aatgacggcg atgcgggagc gctggcagaa
     4921 acatactggg gcgcgggaaa aggaaagagc cgtgtggtgt acgtgacggt gggtacggga
     4981 attggcggcg gcgtgattct cgatggacac ttatatcgcg gcgtagcggg atcgcatccg
     5041 gaaatcgggc accacgtgat ggaagtttcg ggcccggagt gtacctgcgg atttcgcgga
     5101 tgctgggaag cactggcggc gggatcgtcc atggaaaagt ggtatgcgga aaatcggcca
     5161 gcggcggaac gttac
//
LOCUS       DASTPM010000419         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_619134, whole genome shotgun sequence.
ACCESSION   DASTPM010000419 DASTPM010000000
VERSION     DASTPM010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619134"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VFR42_07340"
     CDS             complement(<1..164)
                     /locus_tag="VFR42_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU0369004.1"
                     /translation="MNGALPFIAAAALLALVLGFLARRGKEMNLEQWTVGGRGFGAIF
                     VFLLLAGEIYT"
     gene            complement(161..358)
                     /locus_tag="VFR42_07345"
     CDS             complement(161..358)
                     /locus_tag="VFR42_07345"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEU0369005.1"
                     /translation="MRSIRWLAVVPFLALLIGPFFVNRPTPLILGLPLLLAWIVVWIL
                     ITSLIMAVIYAVDPENRGEGT"
     gene            complement(778..1041)
                     /locus_tag="VFR42_07350"
     CDS             complement(778..1041)
                     /locus_tag="VFR42_07350"
                     /inference="COORDINATES: protein motif:HMM:NF023665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HEU0369006.1"
                     /translation="MHRRLFTGSRNKLDLSNRVQVRIVRKRLKISEQELNNLVGKAGD
                     SIAAVRKEASSRQLLKLPQTHLPPAAVIAQNETTTTENQEVLL"
     gene            complement(1221..1430)
                     /locus_tag="VFR42_07355"
     CDS             complement(1221..1430)
                     /locus_tag="VFR42_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369007.1"
                     /translation="MTEVGLSPPEEARVQQAADEAFRVLTGNEVPPPRRSVRVSQLLR
                     DATLNAPLHALAVAFLLGVLVARRR"
     gene            complement(1871..2080)
                     /locus_tag="VFR42_07360"
     CDS             complement(1871..2080)
                     /locus_tag="VFR42_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369008.1"
                     /translation="MAEPPAKKAEPGGVIPLLFDVNLLHGATRNCSRAARRSLLTTYA
                     NASQQRDWRKTCALAGRSHGSGWGV"
     gene            complement(2094..>2759)
                     /locus_tag="VFR42_07365"
     CDS             complement(2094..>2759)
                     /locus_tag="VFR42_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369009.1"
                     /translation="LFVVGTSIFAAYVGLGIGSSSAAQLAAGADHRQQRDTPPKSSNL
                     GVATAVAAPTAPDSTSDVKPVRRVAGKGPYYVDFRARTAASYGHAFVWFGKTSEKQVE
                     VAGLHPAGDTLPYVLGHLTWVPSETGASYGDLDVQYLTASYRVYLSEPDARKVFAYIK
                     HLQATSPVWNAETTNCTAFIGRIASFMGLKTPFHLLKPEEYVNQLKALNDGRQTVQLA
                     PDT"
BASE COUNT          560 a          844 c          806 g          549 t
ORIGIN      
        1 gtgtagattt ctccggcaag cagcaggaag acgaagatgg cgccgaagcc gcgtccgccc
       61 accgtccact gctcgaggtt catctccttg ccgcggcggg caaggaagcc gaggaccagc
      121 gccagcagtg cggcggcggc gatgaacggc agcgcgccgt tcatgtcccc tccccccggt
      181 tttccggatc gaccgcgtag atcacggcca tgatcagcga tgtgatcaag atccagacca
      241 cgatccaggc gagcagcaac ggcaggccga ggatgagcgg cgtcgggcga ttcacgaaga
      301 acggaccgat gagcagagca agaaatggca cgacggcgag ccagcggatg ctgcgcatgg
      361 cgttcctgct gtggagaaga attattgcgg cgaaaacctg cctgcgcggc gaaagttcct
      421 gtgggaagaa tagactttag tgccgcatcg cagcgcccgt tggatcaccg caggccgcta
      481 ccaatggctt ccatcattgc gcgcggatac gatcgagcac ctcggcgtag ctgcccccca
      541 ctgccggaac agccgcacgc ccgcatacca tggcctgtcg ccgcatcgag cggccagcgg
      601 taacaaggcg tgtagggcaa taagatccag gccggctttc ccatcgcggg tgcgccgtca
      661 cgtgcttccg aatgctcagc tgatggctgc gaacgcgata caggcgacgt aagccaccgt
      721 cacgcccagg ccggccaatc tgataaaatt gcgcgagtcc atactgtcac cttccgctta
      781 aagcagaact tcctggttct cggtcgtcgt cgtttcgttt tgagcgatca ccgctgctgg
      841 tggcagatgt gtctggggca gtttcagcaa ttgtcgcgaa ctcgcctcct tgcgaacggc
      901 cgcgatcgaa tcgccggcct tcccgaccag attgttgagt tcctgttccg aaatcttcag
      961 tcgctttcgt acaatccgca cttgtactcg gttgctgagg tccagcttgt tgcgcgagcc
     1021 agtaaaaagg cgccggtgca tctcgatctc cattgacctg tgagagatca agtccaggcg
     1081 ggctctacgc cgcctggact cgatcggtga ctgtctcgcc gctttgcgaa gatgccgtct
     1141 ccatgagaaa aacttggtcg ggcgttttgc gttccgggcg cgcctcaaat taaacttcgg
     1201 ggtgatttgg agccgcctgc ctaccggcgt cgtgcgacca gcacacccaa caggaaagcc
     1261 accgccagcg catgcaatgg cgcgttgagc gtcgcgtcgc ggagcagctg gctaacgcgc
     1321 acgcttcggc gtggcggcgg cacttcgtta ccggtcagaa cgcggaatgc ctcatcggcc
     1381 gcttgttgca cgcgcgcctc ttccggtggg ctcagtccaa cctctgtcat ttcccaacct
     1441 ccgctggttt acgctctcag cttaatcggc aaacggttgg aagagttccg cggaacgact
     1501 tgaatgctcc agtgttttca aagcggtggc aggctgaatt cagaggatta tcactcgcat
     1561 gccgtcatta cccgaagagg acctgctccc cgtctgcccg cagtgcggtg cctggccgat
     1621 ggctgccaag tcgatggagc cgaaccgcat cacgttcaga tgcccgcagt gtcacgcaga
     1681 ccagaccagg tcgactcgcc ataccggtgg aggacaatgg ggcgtgcgcg cacctgcaat
     1741 tcgcgcgtag tcacccgggc tcgatgggcc agttgagtgc gctgtcaccg cattctgagt
     1801 gcagtccggc accgccgcaa atcgggccac gattttcgcg gcgagctaag ctctcccctt
     1861 cggtcagaag tcaaacaccc catcctgatc catgcgagcg gcccgcaagc gcgcaggtct
     1921 ttcgccaatc ccgttgctgc gatgcgttgg cataggtggt cagcagcgac ctgcgcgccg
     1981 cgcggctgca attgcgcgtc gcgccatgca acagattgac atcgaacagc aagggaataa
     2041 cgccacccgg ctcagccttc ttggctggag gctccgccaa tgaaagagaa aatctaagta
     2101 tccggggcta gttgcaccgt ttggcgtcca tcgttcaggg ctttgagttg atttacgtat
     2161 tcctcaggct tcaacaaatg gaatggagtt ttcaaaccca taaaactcgc tatgcggccg
     2221 atgaacgcag tgcagttcgt cgtctcagca ttccataccg gcgaggtcgc ctgaagatgc
     2281 ttgatatagg cgaagacttt tcgagcgtca ggctcactca aatagacgcg atagctggcc
     2341 gtgagatatt ggacatcaag gtcaccgtag ctcgctccgg tttcagaggg cacccacgta
     2401 agatgtccca acacgtaggg tagtgtatcg ccggcaggat gcaggccggc aacttcgacc
     2461 tgcttctcgc tcgtcttgcc gaaccacacg aacgcatgac cgtacgatgc cgccgtgcgg
     2521 gcacgaaaat ccacgtaata cggtcctttg cccgcaaccc ttcgaaccgg ctttacatct
     2581 gatgtggagt caggggcggt cggtgcagca acggccgtcg ccacaccaag atttgacgac
     2641 ttgggaggag tgtcccgttg ctgccgatga tcagcgcccg ccgctagttg tgctgcgcta
     2701 ctgcttccaa ttcccaagcc cacgtaggct gcgaagatgc tggtcccaac cacaaatag
//
LOCUS       DASTPM010000420         5941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1197049, whole genome shotgun sequence.
ACCESSION   DASTPM010000420 DASTPM010000000
VERSION     DASTPM010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197049"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..974)
                     /locus_tag="VFR42_07370"
     CDS             complement(333..974)
                     /locus_tag="VFR42_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0369010.1"
                     /translation="MNAGSGVRDLLAAGLEALDLLNIGLVVCSATGQLLVANQTAERV
                     LQAGDGLRLDADGRLEGTHDPSGSLKALLRSVAQCSHSQHGGTRDTAFAVARGSGKRP
                     LTLFVRAVTAKSDTRVPDEAAVLCMILDSALPVEATKAELRQLYGFTSTEARLANHLM
                     DGLALEDCCNAMGIRRTTARMHLRNIFAKTGVRRQGELVSLLLKSIGLGPRPK"
     gene            1273..1551
                     /locus_tag="VFR42_07375"
     CDS             1273..1551
                     /locus_tag="VFR42_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369011.1"
                     /translation="MHFEEDDIPLISGTGEAFMDVPGTPATGTRRDEDIALDLMKFIA
                     MTTGYGKTTSSGGGVGFEKISGGASKPEEYAAHLLELYGKCFARVSKK"
     gene            1769..2332
                     /locus_tag="VFR42_07380"
     CDS             1769..2332
                     /locus_tag="VFR42_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEU0369012.1"
                     /translation="MSTTLTDPVALMKQGSKWGIVWGALLVVLGILAIAEPFLAAIAL
                     AGFIASLLVFVGILHIAMAIHAHSGTSMGWKLLVGVSYIFIGSYLLFRPVVGVATLTL
                     MLAFLFLIEGVLDFMLWWKSRSANGAIWILLDCIVTLVLGGMIYVHWPSSSEWAIGTL
                     VGISLIMSGVTRLMLSMAVRKLTTQAA"
     gene            complement(2428..2820)
                     /locus_tag="VFR42_07385"
     CDS             complement(2428..2820)
                     /locus_tag="VFR42_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369013.1"
                     /translation="MSFYDETLYGLDDEMDEYGDTGAYGESPEEILEEEEEEEEEETG
                     GPVISESVVEVEEEIAPAPPKPAGGGGGARKPPAKKKAAKKKAPKKAAKKKAAKKKAA
                     KKKSAKKPAKKAAKKAAKKKSAKKKKRR"
     gene            complement(3030..4709)
                     /locus_tag="VFR42_07390"
     CDS             complement(3030..4709)
                     /locus_tag="VFR42_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU0369014.1"
                     /translation="MEKEYLAGQDNYKAGHLEAARQNFDRAFDLLLSSNLEINSDPRL
                     ENELDRIVEGVNGFETQALQQGDGFTEQKSEPAPIDEANEAAVPVDANVKAKAEAEIK
                     STRSDLPLMMTDQVAGYITYFSSRGRGTLENALVRSGRYEDMIRRVLKQEGVPQDLIY
                     LAQAESGFHPLALSRVGARGIWQFMASRARGYGLQRDWWVDERQDPEKATRAAARHLK
                     DLYQQFGDWYLAMAAYNSGPGTVQSAVKRTGYADFWELYRRNVLPKETRNYVPIIVAV
                     TIMAKNPTQYGLDHLEKAHPVPSDVVKIDYPVDLRLVAECVDASATDLQDLNPSLLRM
                     TTPKDHKFDLYLPAGTKDRYQTAISAIPTDMRVWWRYHKVQPGDTLASIARTYRSTPK
                     AIAEANNLEEGASLTADSKLTIPIAPGKRALTEEATYARHATRYRIRKGDTVRSIADN
                     FGVPPVMVRRWNHLKSDSLKGRSVVYVHLPISPSAAAQSDQVASRSKSKHKKAVIAAK
                     SPSDAVVRHKVKQGETLYSIASTYKTTVDALKRDNRNVATLRPGMVLVVKP"
     gene            complement(5188..5610)
                     /locus_tag="VFR42_07395"
     CDS             complement(5188..5610)
                     /locus_tag="VFR42_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369015.1"
                     /translation="MALELVRTRLKNYPSISQTLEIMPIFFVGVAVLFASLGMGEAVY
                     RLLFSDYDGATDRLPIEAFFGFVFAWLATSFVRNLYKHRQRTIAKLHLIWDRNHQIRA
                     ALEAISPHPHTSRNQQSIRVIREELDRIEGALKEVLPR"
BASE COUNT         1194 a         1695 c         1735 g         1317 t
ORIGIN      
        1 tgccaaaggg aatcctccac agaaccccac acaaaaaacg tggaaatgta tcgttgtcac
       61 gaggcgaggg agaacttccg cccgtgcttg ccatccaatt ttgggggagt ggctaaccgc
      121 cagtggggga ctggcgttct aagaactggc cgcagattag gcgactcctc agtaactgtc
      181 tacgtccaca tggatgatat gaatcaaaaa tcgggacaga aacgttggca gcggcctcgc
      241 aggaaccggc tggccgcaca acatgtaaga ggacgccatc gaatgggaga ctctggaggg
      301 tggaagggac gttcgatcgg ttcggtgagc aactacttcg ggcggggacc cagcccgatg
      361 cttttgagca acaacgaaac cagttcaccc tgccggcgaa caccggtttt cgcgaaaatg
      421 tttctcagat gcatgcgggc agtcgtgcgc cgaattccca tcgcgttaca acaatcttcc
      481 agcgcgaggc catccatcag gtgattcgcg agacgcgcct cggtgctggt aaatccgtag
      541 agctgtcgca gttctgcttt tgtggcttct acaggaagcg cagaatccag aatcatgcat
      601 aaaacggcgg cttcatccgg aacacgcgtg tctgattttg cagtcacggc ccgcacgaat
      661 aaggtcaggg gccgtttgcc cgaaccgcgc gcgaccgcga aggcggtgtc tctggtaccg
      721 ccgtgttgcg agtgcgagca ttgggcaaca cttcgaagaa gtgctttcaa cgaaccgctt
      781 gggtcgtgag taccttccag gcggccatcg gcatcgagtc gcaagccatc gccagcctgc
      841 agaacccgtt ccgcagtttg gttcgcaacc aacagctgcc ccgtcgcgct gcagaccact
      901 aaccctatgt taagtagatc caacgcctca agtcccgcag ccaatagatc gcgtacgccg
      961 cttccggcgt tcattgcggg agatagtccg ggtgccgcgg tgcggtcttc ttcggaggaa
     1021 cgcgcttgcg gcaccgcgaa tccaggagat ccactcttcg gagcgcgtga ttttgagctg
     1081 ggatcgacgg ccaagtttcg acctcagatg cgtgggcagg cgcggttgat ggggaaggaa
     1141 acaacaccgc gccatcagga ccctaggagc atagcatgct cgaatcgatc ggcaggtact
     1201 aggttccaac ttcagtaacc aatcccatgc cccggatggg cctgcggcta tttgttatga
     1261 tcgaaacgtc ttatgcattt cgaagaagac gatattccac tgatttccgg gacaggggag
     1321 gcattcatgg atgtgcctgg cactccggcg acggggaccc gtcgcgacga ggacatcgct
     1381 ctcgatctga tgaaattcat cgccatgaca actggctatg gaaagacaac ctccagcgga
     1441 ggcggcgtgg ggtttgagaa aatcagtgga ggcgcgtcca agccggaaga gtacgccgcg
     1501 cacctgttgg agctttacgg gaaatgtttc gcacgggtca gtaaaaagta gtcgcccgag
     1561 cccatcgcac tagaacgaaa gcacttacgc ctggccgcac caaggcggac aaaacgcgcc
     1621 cgggcgcctg agtccaaatc cgcctcgatc tgagcaatcc ccttttctct cttgcgcttg
     1681 caggggaccg tctgccacaa tgcgccgcac aagtctcatc cgatctcccg ctgacgattc
     1741 aagtcagcaa gctgcggaaa ggaatgctat gtccacgact ctgacggatc cggtagcgct
     1801 catgaaacaa ggttcgaaat ggggtatcgt ctggggcgcg ttacttgtcg tgttagggat
     1861 actggcgatc gccgagccgt ttctggcagc catcgcattg gcgggtttca ttgcgtctct
     1921 gctggtcttt gtcggcatcc ttcacatcgc gatggcgatc catgcgcatt ccggcaccag
     1981 catgggatgg aaactccttg tcggagtttc ctacatcttc atcggtagtt atttgctgtt
     2041 tcggcccgtg gtgggcgtgg cgacgctcac gctcatgctc gcctttctgt ttttgatcga
     2101 gggtgttctc gactttatgt tgtggtggaa atcgcgctcc gcgaacggtg cgatctggat
     2161 tctgctcgac tgcatcgtaa cgctcgtact gggtggaatg atctatgtgc actggccctc
     2221 aagttcggaa tgggcgatcg gaacgctagt gggcatcagc ctgattatga gcggcgtgac
     2281 gcgcctcatg ttgtcgatgg cggtgcggaa actgacaacg caagcagcat agggcgcacg
     2341 tccgcgtcat ccgttgcacg cgtgcaacag gaaagaaaaa agccgcgacc taagccgcgg
     2401 ttttttatct gacgaaagcg atagcactca gcgacgcttt ttcttcttcg cgctcttctt
     2461 cttggccgct ttcttggcag cttttttcgc gggcttcttg gcagatttct tcttggcggc
     2521 tttctttttt gcggcctttt tctttgcggc cttcttgggc gcttttttct tagcagcctt
     2581 tttcttggct gggggtttgc gggcgccgcc accaccaccc gcgggttttg gaggagcggg
     2641 tgcgatctct tcctccactt cgacgaccga ctcgctaatt accgggccgc cggtttcctc
     2701 ctcctcttcc tcctcttctt cctccaggat ctcttccggt gattcgccgt aggcgccggt
     2761 atctccgtat tcatccatct cgtcatcgag tccgtagagg gtttcgtcgt aaaagctcat
     2821 tgctcctcct ggtttgcgga aaggttaacg cgggcacgcc gccgaaccga cgccgcgcat
     2881 agaaaaatag aagactactg gaaaactgcc tcgtcggctg aacggggcgc gattatagca
     2941 gagaacttgg cgttgagcgc taggtcttcg agcatagttt gagcagtttt tatgagtcaa
     3001 aagactcgcg tgcgccaaac gcgtttcgcc tatggtttca cgaccagaac cataccggga
     3061 cgcagcgtgg cgacgttgcg attgtcccgt ttgagggcat ccaccgtcgt cttgtacgtc
     3121 gaagcaatgg agtacagcgt ttcaccctgc ttgaccttgt gacgaacaac tgcgtcgctc
     3181 gggctcttgg cggcaataac cgcctttttg tgcttggatt tagagcgcga tgccacctgg
     3241 tcactctgcg ccgccgcgct cggactgatc ggtaaatgaa cgtagacgac gcttcggccc
     3301 ttcaggctgt cacttttcag atggttccag cgccggacca tcaccggagg cactccgaag
     3361 ttgtcagcga tcgaccgcac cgtatcgcct ttccgaattc tgtagcgggt tgcgtgacgc
     3421 gcgtaggtgg cttcttcggt gagggcgcgc ttgccgggtg caatcggaat cgtcagctta
     3481 ctgtcggcag tgagactggc accttcctcc aggttgttgg cctcggcaat ggcttttgga
     3541 gtactgcggt aggtacgcgc aatggaggcc agcgtatcgc ctggttggac cttgtgatag
     3601 cgccaccaga cgcgcatgtc agtcggaatc gcggaaatcg ccgtctgata ccggtctttc
     3661 gtccccgcag gcaagtacag gtcgaacttg tgatccttcg gcgtggtcat gcgcagaaga
     3721 ctgggattca ggtcctgaag atcggtggct gaggcgtcca cacactcggc aaccaggcgc
     3781 aggtcgaccg ggtagtcgat tttcacaacg tccgaaggga ccgggtgcgc tttctcaaga
     3841 tgatccaatc cgtactgcgt cggatttttg gccatgatgg tcacggccac aatgatcgga
     3901 acgtagttgc gggtttcttt cggcagcacg ttgcggcggt agagctccca gaagtcagcg
     3961 tacccggtcc gcttcaccgc gctctgcact gtgcctggcc cggaattgta ggcggccatc
     4021 gccaggtacc agtcgccaaa ctgttggtag aggtctttga ggtgccgggc ggccgcgcgc
     4081 gtcgccttct ccggatcctg tcgctcatcg acccaccaat cgcgctgcag gccgtagccc
     4141 cgggcccggc tcgccatgaa ctgccaaatg ccgcgcgcac ctacgcggga aagcgccagc
     4201 gggtgaaatc ccgactcggc ttgtgccagg taaatcagat cctgcgggac gccctcctgc
     4261 ttcaggacac gtcgaatcat gtcctcatag cgtccggaac ggaccagggc gttttccagc
     4321 gtcccccggc cccggctcga gaaatacgtg atatagccgg caacctggtc ggtcatcatc
     4381 agcggcagat cggagcgtgt ggacttgatc tccgcttccg ccttggcctt gacattggca
     4441 tcgaccggca ccgccgcttc gtttgcctca tcaatgggag ccggctcgga cttctgttcc
     4501 gtaaagccat cgccctgctg cagggcctgt gtttcgaatc cgttcacccc ctcgacgatc
     4561 cggtcgagtt cgttttccaa ccgtggatcc gagttgatct ccaagttgct gcttagcaac
     4621 aggtcaaatg cacgatcgaa gttctgacga gccgcctcga gatgcccggc cttgtagttg
     4681 tcttgcccgg cgaggtattc cttctccacc ttggcgatca ggtcagcgac cggatcaaca
     4741 cttgcaacag tcgaggtctg tcccgctggc agggcaactt gcttggctgg cgaaacctga
     4801 ttctgagagg ctggcgcatc aagcggaatg gtggctacag ccggaggcgc agattgcttc
     4861 ggtgagagta agggggccgg atggtgtgcg gattgacacg cgcaggaggc cagcagagct
     4921 gccaggatcg cgatcgcgac atagagtttc gggaatctca tatttctcta tgacttaccg
     4981 cgtccagcct cccgaggttt ggatgcagaa aacctgcaaa atggtaaccc cggaactttt
     5041 gcagggtcaa ttcacaaaag acctacatct gcattacttt cggcataaac cctaggggga
     5101 tcagagacta caaagggcgt tagcgcacgg tccaaatttt gcacagaccc tcgagccagg
     5161 atggatcgag ccgttccgct ccttaatcta ccgaggaaga acctctttca gcgctccttc
     5221 gatgcggtcc agttcttcgc gaatgacccg gatcgactgc tggttccggg aggtgtgcgg
     5281 atgcggagag atcgcctcca gcgccgcgcg aatctggtga ttgcgatccc aaatcaggtg
     5341 gagcttggca atggtccgct ggcgatgctt gtagagattg cgaacgaacg aagttgcaag
     5401 ccaggcaaaa acaaatccga agaacgcttc aatcggcagg cgatctgtcg cgccatcgta
     5461 gtcggaaaag agcagccggt aaaccgcttc gcccatcccg agcgatgcga acagcactgc
     5521 cacgcccaca aagaatatgg gcatgatttc cagagtctgg ctaatcgaag gatagttttt
     5581 gagccgggtc ctcaccaatt ccaatgccat gccaacaccc ttagttcgaa tttagcgcgg
     5641 gaggccccgc gggaacgccg tcatggtaaa ccagccagac cgggtgggga aaggggcgtc
     5701 gcgcagaaat tatgacatga tgtagtctcg ttgagggtcg cccggaacgc ctcttcagcg
     5761 cccggctgat gccttttcgg cgaggcagct tccgccaccg gagctatgct cagcttgttg
     5821 accatcatcg gcgtgggatt cgtgctgggg atccggcacg cgaccgaccc ggaccacgta
     5881 atcgcggtca cgaccatcgt gagccgtcag cagagcatcc ggcatgccgg cctgattgga
     5941 g
//
LOCUS       DASTPM010000421         5179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_995173, whole genome shotgun sequence.
ACCESSION   DASTPM010000421 DASTPM010000000
VERSION     DASTPM010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995173"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /gene="thrC"
                     /locus_tag="VFR42_07400"
     CDS             complement(<1..758)
                     /gene="thrC"
                     /locus_tag="VFR42_07400"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU0369016.1"
                     /translation="MPEIYQLRCRECGKLWGNTPRSFCEECFSPLEVGYDYQALKSIA
                     SKNKVAGRSFDMWRYAELLPLPEGYTYPNSVGGTPLVPSKHLAKKWGLRNLYFKNDAV
                     CFPSLSFKDRVVATALAAAGRFGFRTVGCSSTGNLANAVAAQSAQQGFDACVFIPADL
                     EPAKILNTAVYGARIVRINGNYDHVNRLCAQIADRFQWGLVNVNLRPYYAEGSKTHGF
                     EIAEQLGWKLPDNVVVPMAGGSLITKIAKAFRELV"
     gene            1416..4244
                     /locus_tag="VFR42_07405"
     CDS             1416..4244
                     /locus_tag="VFR42_07405"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEU0369017.1"
                     /translation="MLLQLERSPQQEAALQLFIEQLQTKGSPSFHHWLTAQEFGARYG
                     VDQLDLEAVTAWLRSEGFQVNVVYPNGMLIDFSGTAGQVRKAFQTEIHHLAVEGNMHI
                     ANMSEPRIPAALAPVIAGVVSLHDFRPRPMHQLRKPRPTFTFGGLFGDTYAVVPADLA
                     TIYNLNPLFNAGISGQGQSITLIEDTDVFTANDWNTFRSVLGLSAYSGASFSSVHPAP
                     ASGTNNCNPPGAFAPNDAEAILDAEWASAAAPSAANQMAACADTSTTFGGLIALQNLI
                     NSSAPPAIVSISYGQCETVNGAAANAAYSSAYQQAVAEGVSVFVAAGDSGAAGCDNGA
                     AVATHGIGVNAFASTPYNVAVGGTDFSDTFSGTNLNYWNSTNTPAFGSAISYVPEIPW
                     NDSCAGLLVSAYLGYSPTYGPTSLCNDPLLGALLESTVAGGGGPSQCATGSPSTSGVV
                     SGSCAGWLKPYWQTVVGNPNDGVRDTPDVSLFAADGLWSHYYVFCWSDTAHGGAACGN
                     DPSAWSGAGGTSFAAPVMAGIQALINQRAGGPQGNPAPVYYQLAAAEFGSTGSATCNA
                     SNGAGVSSSCTFYDVTDGDIDVDCSGPDCYLGGASVGVLSTSNSAFSPAYGTSVGWDY
                     ATGIGSVNATNLVNNWPATAPAENFSLSASPGTVTVAQGVSVSTTITITPLDGFNGNV
                     ALAASGLPAGVTASFNPSSAQSSSTLTFAASAGAATGSVTVSVTGTSGNLTNQTQVTL
                     IVNPAGNYALSASPNVLTIAQGSHGTSSITIAPLNGFSGAVSLSASGLPSGVTASFNP
                     VSTTSTSMLTLTAGASAATGTFTVTINSVSGNLNKSTTISLTVTPAPNFSLSASPTSL
                     SIARRSSGTTTITITPANGFSSGVTLSASGMRNGTTASFSPNPTTGSSTLTLSASRKA
                     STGTVTITITGKSGNLTQQATVSLTVDR"
     gene            4592..>5179
                     /locus_tag="VFR42_07410"
     CDS             4592..>5179
                     /locus_tag="VFR42_07410"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0369018.1"
                     /translation="MGEPEKGKTWNVLGWVRREKAVAALFVALTLVVGILIGTVVSGH
                     TSAMKTMTFAGKGATPLPMPDAIPSSNSFASIVNRVEPAVVNISTTQVMEKRQTLNKK
                     RRQAPNPNGDDDPMQDFFDRFFDGRNGGQDAPPAAERSLGSGVIVDRKGFILTNNHVV
                     EQATKIQVQLNGDTTRYNAKVVGTDEETDLAVIKIE"
BASE COUNT         1089 a         1586 c         1459 g         1045 t
ORIGIN      
        1 accagctcac gaaatgcttt cgcaatcttg gtaatgagcg agcctccagc cataggcacc
       61 acgacgttgt cgggcagctt ccagccaagc tgttcggcga tttcaaagcc gtgggttttc
      121 gagccctccg cgtagtaggg tcgcagattt acattgacca ggccccactg gaagcggtcg
      181 gcgatctgcg cgcacaagcg gttcacgtga tcgtaattcc cgttgattct cacgatgcgc
      241 gcaccgtata cggcggtgtt caggattttt gcgggctcca ggtcggcggg gatgaagacg
      301 caggcgtcaa aaccctgctg cgccgattgc gccgcgacgg cattcgccag gttgcccgtg
      361 gacgaacagc cgaccgttct gaagccgaag cggcccgcgg ccgcaagcgc agtggccacg
      421 acgcgatctt taaaggacag ggaggggaag cagacggcgt catttttgaa atacaggttg
      481 cgcaggcccc attttttcgc cagatgcttg gacggcacaa gcggtgtgcc gccaaccgaa
      541 ttcgggtagg tgtagccttc gggcagcggg aggagctcag cgtaacgcca catatcgaag
      601 ctgcggccgg ccaccttgtt tttactggcg atggatttaa gtgcctggta atcgtagccg
      661 acttcgagag gggaaaagca ttcttcgcag aacgaccgcg gcgtattgcc ccacagcttc
      721 ccgcattccc gacaacgtaa ttggtagatc tccggcatag ccatgtccaa tcgtggcccg
      781 agacagcaca agccaacgga attcgccgta gcggcaaacg aagcgcgaaa gagttagggc
      841 agcgcggggg gggaacaaaa aagccctccg tgccaaagag tgcaggaggg tctcagaaat
      901 ccttcaagaa actctaccag ctctttagca gttttttacg tggagcaagt cgccctaaat
      961 cgccacgttt cgccgcagca aacggcagac ttaattaatg acagggaagc actccgttgt
     1021 caatagcttc cgtacgcgcc gcataccgca aatgattctg gaccttgaag acggctgacc
     1081 taaaactgtt cttgggggcg gttcccccgg tcgtcttgcc ctgctatcac agcaacagcc
     1141 gattgtctgc gttctgcgtg gtcaagtcaa agacgttcct caggaggggc cccatgatcc
     1201 gtaagctggg actcgcttcc gtatctttcg cagctatcct ggcctgcttc gcgcctgaca
     1261 ctgccgcgca atccagggac ggagatctct cgagaccccg gatcagcagt gcggtcgatc
     1321 gagcaagcca ggtcgcgctc aggggcaacg ttcggccaga agccaggccc gaaaacgatc
     1381 gcggccgtgt tgtcgactcg gttcccttgc aacacatgct gctccagctg gagcgttccc
     1441 cccagcagga agcagcgctc cagctattta tcgagcagct tcaaaccaag ggctcgccca
     1501 gttttcatca ctggctgacc gcgcaggaat tcggcgcgcg ctacggcgtc gaccagctgg
     1561 acctcgaagc tgttacggcc tggctaaggt cagaaggctt tcaagttaac gtggtctatc
     1621 caaacggcat gctcatagac ttttccggca ctgcgggcca ggtgcgcaaa gcatttcaaa
     1681 ccgaaatcca ccatctggcg gtcgaaggca acatgcacat cgccaacatg agcgagccgc
     1741 gtattcctgc cgcgttggcg cccgtgatcg cgggcgtcgt ttctttgcac gatttccgcc
     1801 ctcgtcccat gcatcaattg cgcaagcccc ggcccacatt cactttcggc ggactctttg
     1861 gcgatactta cgccgtggtt cccgccgacc tggcgaccat ttacaacttg aatccgcttt
     1921 ttaatgctgg gatttccggc cagggtcagt ccatcacact gatcgaagac actgatgtct
     1981 tcaccgcgaa cgactggaat acgttccgat ccgtgctggg cctgtccgca tactcaggag
     2041 cttcgttttc gtcggtgcat cctgctcccg ccagtggcac caacaattgc aacccgcccg
     2101 gtgctttcgc gccgaacgat gccgaagcca tcctggatgc ggaatgggcc agcgccgccg
     2161 cgcccagcgc cgccaaccaa atggccgcct gcgccgacac cagcaccact ttcggtggct
     2221 tgatcgctct ccagaatttg atcaatagca gcgcgcctcc cgccatcgtc agcatcagct
     2281 acgggcaatg cgaaaccgtt aacggcgcgg ccgccaacgc cgcgtattct tcagcctacc
     2341 aacaggcggt agcggaaggc gtttcggttt tcgtcgctgc gggcgatagc ggagccgctg
     2401 gatgcgacaa cggcgccgcg gtcgccactc acggaatcgg cgtcaatgct ttcgcctcga
     2461 ccccttacaa cgtcgcggtt ggcggcacgg acttcagcga caccttctca ggtaccaacc
     2521 tcaattactg gaattccacc aacactcctg cttttgggtc ggcgatctct tacgtacccg
     2581 aaattccctg gaacgattcc tgcgccggtc tgctggtatc cgcttacctc ggctacagcc
     2641 cgacctacgg ccccaccagc ctgtgcaacg atccattact gggcgcgctg ctcgaaagca
     2701 cggtcgccgg tggcggcggc ccgagccagt gtgctacggg cagtccgtcg actagcggcg
     2761 tcgtcagcgg ttcctgcgcc ggttggctga agccttattg gcaaactgtt gttggtaatc
     2821 ccaacgacgg cgtccgcgat acgcctgacg tttcgctatt tgccgcggac ggcctttgga
     2881 gccattacta cgtgttctgc tggtcagaca cagcacacgg cggcgcggcc tgtggcaacg
     2941 atcccagcgc gtggtctggc gccggcggca cttccttcgc cgcgccggtc atggcgggca
     3001 tccaggcgct gatcaatcag agagcaggcg gaccgcaagg caaccctgct cccgtttatt
     3061 atcaactggc cgcggctgaa tttggttcga ccggcagcgc cacctgcaat gcatctaacg
     3121 gcgcgggggt tagctcgtcc tgcaccttct acgacgtcac cgacggcgac atagacgtgg
     3181 attgtagcgg tcctgactgc tacttgggtg gggcatcagt cggtgtgctc tccacctcga
     3241 atagcgcgtt cagcccagcg tacggaacgt ccgtcggttg ggactatgca accggcatcg
     3301 gcagcgtgaa tgcgacgaat cttgtgaaca actggccggc gacagcgccg gcggagaatt
     3361 tttcgctatc cgcttctccc ggcactgtga ccgttgcaca aggcgttagc gtgagcacga
     3421 caattacgat cactcctctc gacgggttca acggcaatgt ggctctcgct gcgtctggac
     3481 tgcctgccgg cgtgaccgcg tctttcaatc cgagcagcgc tcaatccagc agcaccttaa
     3541 cctttgccgc ttctgcaggc gcagcgactg gttcagtcac ggtgtccgtc accggcactt
     3601 cgggcaattt gaccaaccag acccaagtaa ccctgattgt gaatccggcg ggcaactacg
     3661 cgctttccgc ttcgccgaat gtgctgacga tcgcgcaggg aagccacggc accagcagca
     3721 ttacgatcgc ccctttgaac ggcttcagcg gggccgtcag tctgtcggct tctggtctgc
     3781 cgagcggcgt aactgcatcc tttaatccgg tcagcacaac cagcaccagc atgttgacac
     3841 tgactgctgg tgccagcgcc gccacaggaa ccttcaccgt caccatcaac agcgtcagcg
     3901 gcaatctaaa caagtccacg acgatttcgc tgactgtgac tcccgcgcct aatttcagct
     3961 tgtcggcgtc ccctacgagc ctgagcatcg cacgcaggag cagcggcacc actaccatca
     4021 ccattacacc ggcgaacggg ttcagctccg gcgtaacctt gtcagcttcg ggcatgcgca
     4081 acggaacaac cgcctccttt agcccgaatc cgacgaccgg ctctagcact ttgactttat
     4141 ctgcgagtcg taaggccagc acgggaaccg tgacaataac cattaccggt aaatcaggaa
     4201 acctcaccca gcaggctacg gtcagcctaa cggtcgatcg gtgagcgtca acaaatcgcc
     4261 acaaacgtaa agtcctatcc ctgcaacagt tacccggcta ggggcgccat cggctgtcaa
     4321 tagtattttt gaatgggcgc ggacggtaac aacaaaatcg acctttcttg cgtcaaacta
     4381 gggagtagta ggcatcaaat ttacagcgcc tccaaagcca ccccggccga aaggccagtg
     4441 tgttttccag cgcagcctga actttgcgct gggcaactct gggaaaatca cggtgttata
     4501 ctgggcaggt agggtgcggt ccaatggaat cagggatgag ccctgaaacc cgagcgagtc
     4561 ggcggcggag agaaagacag gagttttaga aatgggcgaa ccggaaaagg gtaagacctg
     4621 gaatgtattg ggttgggtgc gacgcgagaa ggcggttgcc gcgttatttg tcgcgctcac
     4681 tctggttgtc ggaattctga ttggtacggt tgtttcgggc cacacctccg cgatgaagac
     4741 catgacgttt gcaggcaagg gagccacacc gctacccatg cccgacgcca tcccgtcttc
     4801 caattctttt gcttcgattg tgaatcgtgt ggaacccgcc gtagtgaata tctccaccac
     4861 gcaagttatg gagaagcggc aaacgttgaa caaaaagcgc cggcaggcgc caaacccgaa
     4921 tggcgacgac gatcccatgc aggatttctt tgatcgtttc ttcgatggac gcaatggcgg
     4981 gcaggacgcg cctccggcag cggaacgcag cctcggctct ggcgtgatcg tggatcgcaa
     5041 aggttttatc ctcaccaaca accacgtggt tgagcaggcc accaagattc aagtccagtt
     5101 gaacggcgat acgacgcgct acaacgccaa ggtagtcggc acggacgaag aaaccgatct
     5161 ggccgtcatc aagatcgag
//
LOCUS       DASTPM010000422         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_619262, whole genome shotgun sequence.
ACCESSION   DASTPM010000422 DASTPM010000000
VERSION     DASTPM010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619262"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFR42_07415"
     CDS             complement(<1..295)
                     /locus_tag="VFR42_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369019.1"
                     /translation="MNTHDQFRELIEAYAIGSLDAADRAALEAHLAAGCSECGKALEE
                     ARWLVSHLAYLAPNAEPSGLLKGRLLQSARSEAVAHPLPQPAVPRYAVPWWLWA"
     gene            complement(292..879)
                     /locus_tag="VFR42_07420"
     CDS             complement(292..879)
                     /locus_tag="VFR42_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0369020.1"
                     /translation="MFLAAFSPKGNDEVSPDATLMQHLLRRDVSAFEELYERHSRIVY
                     SLVLRILRQGTTAEEVVQDVFLQLWRNSAQYDRSRPFVPWLLTLARNRALDQLRLKSE
                     RQRRREDQAEELPQVAVAAPEYEKALDEKRRAERVRSLIGSLPAGQKKCIELAYFEGL
                     SHSEIADSLREPLGTVKSWIRNGLIRLKEGLQAAP"
     gene            complement(978..>2200)
                     /gene="hemG"
                     /locus_tag="VFR42_07425"
     CDS             complement(978..>2200)
                     /gene="hemG"
                     /locus_tag="VFR42_07425"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU0369021.1"
                     /translation="DGFLLELGPQSFSGTAALRTMCADLGIEDQVVHAPARAPRYVLI
                     DGALKAVPLTPPAMLTSSLLSLKTKWSVARDVFGRSSPPDNDESIASFVRRKFGAELL
                     DRLVGPFVSGIYAGDPEKLSLRSSFPQLHEAEKSAGSVIRGMMRAAKSRKGPRERPAL
                     LSFRDGNETLVRGLATKLGSHLQLGAEVLGIAIRNQAGKPGFELSIRQASGDALLVSD
                     HLVLATSADVAGMLLRDVNPAFEPLLAGIEYAPVAVVSLSYRREDIGHSLDGFGFLAP
                     RSSGLRLLGCIWNSSLFPKRAPANHVLLTSFVGGATDPAAATLSAKDLRDLVHRELTP
                     LLQIRASVAFSNVQIYRQALPQYNLGHADRLLALEKLRRSVPGLFFVGNYLQGPAIGS
                     CVDLAIATAQAIAG"
BASE COUNT          438 a          628 c          724 g          410 t
ORIGIN      
        1 cggcccatag ccaccacggc acggcgtacc gcgggacggc gggctgagga agcgggtggg
       61 caacggcttc cgagcgtgcg gactggagta gacgcccttt cagcaggccg gaaggctctg
      121 cattgggcgc gagataggcg agatggctga ccagccagcg cgcttcttcc aacgctttcc
      181 cgcattcgga gcaaccagcc gccaggtggg cctcgagcgc ggcgcggtcc gcggcgtcga
      241 gcgagccaat ggcgtaggct tcgatcagct ctcgaaattg gtcgtgggtg ttcacggtgc
      301 ggcctgcaaa ccttctttca aacggatcaa tccattgcga atccaacttt tcacggtgcc
      361 caatggctcg cgcagcgaat cggcgatttc ggaatggctc aggccttcga aataggccag
      421 ttcgatgcac ttcttctgcc ccgcaggaag cgagccgatt aatgagcgca cgcgctcggc
      481 gcggcgcttt tcgtcgagcg ctttctcata ttccggggcg gcgaccgcga cctgcggcaa
      541 ttcttcggcc tggtcttcgc ggcggcgctg ccgttcgctt ttcaagcgca gctgatcgag
      601 ggcgcgattg cgcgccaggg tcagcagcca gggcacgaac gggcggctgc ggtcatattg
      661 cgcggagttg cgccaaagct gcagaaacac gtcctgcacg acttcttcgg cggtggttcc
      721 ctgccgcaga atgcgcagca ccagcgaata gacaatgcgc gaatgccgtt cgtagagttc
      781 ttcgaaagcg ctcacgtccc tgcgaagaag gtgctgcatc agcgtcgcgt ccggcgacac
      841 ttcgtcgttg cccttgggag agaaagccgc caggaacacg ttttctgctt cttcggctcc
      901 tgaggaacta tacgtgaaat tttggagaat ggatgggggc cggaggtgtg agcgcagtct
      961 tgctgcgggg attggcctca gcctgcaatt gcctgagcgg ttgcaattgc tagatcaacg
     1021 cagcttccaa ttgccgggcc ctgcaagtag ttcccgacga agaacaggcc cgggacggag
     1081 cgacgaagtt tttcgagggc tagaagacgg tctgcatggc ccagattata ttgcgggaga
     1141 gcctggcggt agatttgcac gttagaaaac gcgacgctgg cccgaatttg caagagcggg
     1201 gtaagttcgc gatggaccag atcgcgcaag tccttcgcgg agagcgtggc ggcggcaggg
     1261 tcggtggctc ctccgacgaa actggtgagc agaacatggt tggcgggtgc gcgttttggg
     1321 aacagcgatg aattccaaat gcaacccagc aggcgcaggc cgctggatcg cggggccagg
     1381 aagccgaaac catcgaggga atggccaatg tcttcgcggc ggtagctgag cgacacgacg
     1441 gcgaccggcg cgtattcgat gccggccagg agtggctcga acgcggggtt cacgtcgcgc
     1501 agcaacatac cggcgacgtc ggcggatgtg gccaggacca gatgatccga cacaagcagc
     1561 gcatcaccgc ttgcctggcg aatgcttagt tcgaagccgg gcttccctgc ctggtttcgg
     1621 atggcaatgc ccagaacttc ggcaccgagt tgaagatgcg aaccgagttt cgtagcgaga
     1681 ccgcgcacca acgtttcgtt gccgtcgcga aaactcagca aggccggccg ctcgcgtggc
     1741 ccttttcggg attttgccgc tcgcatcatg ccgcggatga cgctgccagc ggatttttcg
     1801 gcttcgtgaa gctggggaaa agagctccgc aagctgagct tttcgggatc gccggcgtaa
     1861 attccagaaa cgaagggacc caccaaacga tccaaaagtt cggcgccgaa tttccggcgc
     1921 acgaacgatg cgatggactc gtcgttatcg ggagggctgg acctgccgaa gacgtcgcgc
     1981 gcaacgctcc actttgtttt cagcgagagc agcgaggaag tgagcatcgc gggaggagtc
     2041 agagggacgg ctttcagcgc gccgtcgatg aggacgtacc gaggagcgcg tgccggcgcg
     2101 tgtacaactt gatcttcaat tccgaggtcc gcgcacatag tgcgcagagc tgccgtgcca
     2161 ctgaaacttt gcgggcccaa ctccagcaga aatccgtcgc
//
LOCUS       DASTPM010000423         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1139385, whole genome shotgun sequence.
ACCESSION   DASTPM010000423 DASTPM010000000
VERSION     DASTPM010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139385"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFR42_07430"
     CDS             <1..881
                     /locus_tag="VFR42_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU0369022.1"
                     /translation="NVLKKAGLPVTLKMRHDAHYIEQLTSFSGAAVGRMIRVDQIRPN
                     PEQPRKALGDLGELTDSVRSKGVLEPLLVRFVPSEGCFYIISGERRYHAARAAGLREV
                     PCIEKTADEAETLEIGLIENIQRKDLTPFEEADGLHRLATQFEYTHEDIARKIGRARS
                     SVTETLSLRNIPEALRKKCIEHGISSKSLLLQIARQPTEKKMVEMFQRILQGGLTRDE
                     ARRERREDQAGPQRPQPFIFHFEPQNETFKFRIQFKKSHVSRDELIVTLREILAQLEG
                     SGSQSDSGDHADSTAA"
     gene            975..1964
                     /locus_tag="VFR42_07435"
     CDS             975..1964
                     /locus_tag="VFR42_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HEU0369023.1"
                     /translation="METFDLICIGAGPTGLACAIEAVRAGLKPVVIDKAALCNSIFHY
                     PVNMVFFTTPELLEIGDLPLVCAAEKPTRVEALKYYRKAAEHYGLTLKLYEQVTHLDG
                     KDGHFIVSTKTDQGHQRAYRGAKVVVATGYYDLPNELGIPGENLPHVSHYYTEPHEFW
                     NQDVVVVGGKNSAAEAALDLWRNGARVSMVHRKAELGSTIKYWVRPDIQNRISTGQIP
                     MYFESYIREITADSVLIEDGAGEKRLPAKQVFLLTGYHPDFAFIESFGVTLDSVTKKP
                     EMDPETHESNVPGLYLAGVVIGGRHTSEIFIENGRFHGKQIVQALTARKTQTA"
     gene            2012..2485
                     /gene="nuoI"
                     /locus_tag="VFR42_07440"
     CDS             2012..2485
                     /gene="nuoI"
                     /locus_tag="VFR42_07440"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU0369024.1"
                     /translation="MASPIREVAETFSTLAKGFSVTFRNMFRKTVTENYPHEPVHFQP
                     RYRGIHVLHRDENGLEKCVGCFLCAAACPANCIYIEAAENTEANRISAGERYASVYNI
                     DYSRCIFCGYCVEACPTDAITHGHGFELAPYDINSLVYRKEQMLEKDILPKTATR"
     gene            2566..>2933
                     /locus_tag="VFR42_07445"
     CDS             2566..>2933
                     /locus_tag="VFR42_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369025.1"
                     /translation="MARITRYPAASISAQLRQLRNLRHGGVRLQKRCRASAKAPRSPV
                     EVEFASRMVMFRLFYLGLTAYDSARHFLGLSELSWSKWTEDVRQRCGKEIPRRGMFPP
                     REYFSEAAGRQSPKAFSALQ"
BASE COUNT          686 a          789 c          853 g          605 t
ORIGIN      
        1 ccaacgtgtt gaagaaagca gggttgcccg tcacgctgaa gatgcggcat gacgcgcact
       61 acatcgaaca actaaccagc ttcagcggcg ccgcggttgg ccgcatgatt cgtgtggacc
      121 aaattcgccc gaatccggag cagccgcgca aggcgttggg agacctgggc gagctgaccg
      181 attccgtgcg ctccaagggc gttctcgagc ccctgctcgt tcgttttgtg ccgagcgaag
      241 gctgcttcta catcatttcc ggcgagcggc ggtatcacgc ggcacgcgcg gctggactgc
      301 gcgaagtacc gtgcatcgaa aaaacagcag atgaagctga gacgctcgaa atcgggctga
      361 ttgaaaacat ccagcgcaag gacctgacgc cctttgaaga agccgacgga ttgcaccgct
      421 tggcgaccca atttgaatat acgcatgaag acatcgccag gaagatcggg cgtgctcggt
      481 cgtcggtgac ggaaacgttg tctttgcgga acattccgga agcgttgcgg aagaaatgca
      541 tcgagcatgg aatctcttcg aagtcgctgc tactgcagat cgcgcgccag ccgaccgaaa
      601 agaaaatggt cgaaatgttc cagcgcattc tgcagggcgg gttgacgcgc gatgaagcac
      661 gccgcgaacg ccgcgaggac caggccggtc cgcaacggcc gcaacccttc atcttccatt
      721 tcgaaccgca gaacgagacg ttcaagttcc gtattcaatt caagaagagc catgtttcgc
      781 gcgatgagct gatcgtcacc ttgcgcgaga ttcttgcgca gctcgaaggc tctggctcgc
      841 aatctgattc aggcgaccac gcggattcca ccgctgccta acgcaattta aacgaattga
      901 agttacaacc tgaatgcaat ggtgcgttga ggccgatttg cttttccgct gcctgctcgg
      961 caaactaatt ttctatggaa acgtttgatt tgatctgcat aggagcaggt cctacaggat
     1021 tggcttgtgc catcgaagcg gtgcgcgccg gattgaagcc ggtcgtgatc gacaaagctg
     1081 cgctgtgcaa ttcgattttc cactatcccg tgaacatggt gttcttcacg acgccggagc
     1141 ttctcgaaat cggagatttg cctctcgtct gtgccgcgga aaagccgacg cgagtggaag
     1201 ctttgaagta ttaccgcaag gcggcggaac actacggctt gacgctaaag ctttacgagc
     1261 aggtgaccca tctggatggc aaggacgggc atttcatcgt ctccaccaag acagaccagg
     1321 gacaccagcg cgcgtatcgg ggcgcgaagg tagtcgtagc gacgggttac tacgatctgc
     1381 ccaacgagct ggggattccc ggcgaaaatc tgccgcatgt ttcgcactac tacaccgagc
     1441 cgcacgagtt ctggaatcag gatgtcgtcg ttgttggtgg aaagaattcg gcggcggaag
     1501 ccgccttgga cctttggagg aacggtgcgc gggtgagcat ggtgcaccga aaggcggagc
     1561 ttggctcaac gatcaagtat tgggtaaggc cggatattca gaaccgcatt tcgacgggac
     1621 aaattccaat gtacttcgaa tcctatatcc gggaaatcac agccgacagc gtactgatcg
     1681 aagacggagc aggggagaag cggctgcctg cgaaacaagt attcttgcta acgggctatc
     1741 acccggattt cgcgttcatt gaaagcttcg gagttacctt ggattcagtc acgaaaaaac
     1801 cggaaatgga tcccgagacc catgagagca acgtgccagg gctgtacctc gcaggtgtag
     1861 tgatcggcgg aaggcatacc agcgaaatct ttattgagaa cggtcgcttc cacggcaaac
     1921 agatcgttca ggcgttgacc gcacgaaaga cgcaaaccgc gtgaacccgg cccggtttgc
     1981 gcgacataac tgcgtttgtt aaactctccg catggcatcc cccattcggg aagtagcgga
     2041 aacattcagc acattagcca agggattcag cgtgaccttt cggaacatgt tccggaaaac
     2101 ggttacggag aactatcccc acgagccggt gcattttcag ccgcgctacc gtgggatcca
     2161 tgtgctgcat cgcgacgaaa acggcctgga aaagtgcgta ggatgctttc tgtgcgcggc
     2221 tgcttgcccg gcgaattgca tttacatcga agctgctgaa aacacggaag cgaaccgtat
     2281 ttccgccggc gagcgatatg cctcggtgta caacatcgat tattcacgct gcattttttg
     2341 cggttattgc gtggaagcgt gcccgaccga tgccattaca cacgggcacg gctttgaact
     2401 ggcgccttac gacatcaact cactggtcta ccgcaaagag cagatgctgg aaaaagacat
     2461 cctccccaaa actgctacac gataaaggaa ttccgttcgt tgcggcgctc cgcttatccg
     2521 tgtccatcaa acgataacta agaaatttgg ctagctctca aggagttggc gcggatcacg
     2581 cgatatccgg cggccagcat ttcggcgcaa ctgcgtcagt tgcggaacct gcggcacggc
     2641 ggggttcggc tgcagaagcg ctgccgcgcg tcagcgaaag ctcccaggtc gcccgtggag
     2701 gtggagttcg cgtcgcgcat ggtgatgttc cgcttatttt atctgggatt aacggcatac
     2761 gattcggcgc ggcattttct cgggctaagc gaattgagct ggtccaaatg gacggaagat
     2821 gtgcgccagc gctgcggcaa ggaaatcccg cgtcgcggca tgttcccccc gcgggagtac
     2881 tttagcgagg ccgctggaag gcaaagtcca aaagcgttta gcgccttaca gca
//
LOCUS       DASTPM010000424         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_43911, whole genome shotgun sequence.
ACCESSION   DASTPM010000424 DASTPM010000000
VERSION     DASTPM010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43911"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..516
                     /locus_tag="VFR42_07450"
     CDS             82..516
                     /locus_tag="VFR42_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369026.1"
                     /translation="MGAILYVGIKLFAYMAWMYLGLRTLRSGAKSSMWCAIFLGILRL
                     FLGLTFALVIYFLSSAVLSRLGSGLAPNVVTYLAVYVPVRWLEWSMMAALLMSPSLSQ
                     FILGTARTDRLWRLGGIAISCLADIPLIVSLGGVIPTGRFLC"
     gene            704..1750
                     /locus_tag="VFR42_07455"
     CDS             704..1750
                     /locus_tag="VFR42_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369027.1"
                     /translation="MTKSAYTTQPIALGEVATYPLTSRKSKVSIADFAKPTTKSNVSL
                     RNFLDSLPNILAAQDLRSLLSAIHAAKRSRKPILWGLGGHVIKTGLGPTLADLMHRGL
                     VSGIAMNGAALIHDFEIALSGGTSEDVDAGLCEGQFGMATETGFYLNQIASTANFSAI
                     GYGEAAGKFLAAQTRRLAPQNAAASILYNAYRHKIPVTIHLAIGTDIPHMHPAADGAS
                     LGAATHHDFRLFCSLVKAMHPGGVYLNWGSAVLLPEVFLKAVSVVRNLGVPLRPITTA
                     NFDFIQHYRPLQNVVKRPTAPAAGRTSASKGYAITGHHELLLPLLAAALASNWPRAGA
                     SAKRQSTKKAPRSK"
     gene            1747..2796
                     /locus_tag="VFR42_07460"
     CDS             1747..2796
                     /locus_tag="VFR42_07460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEU0369028.1"
                     /translation="MNPFDEKPPHSSPDDQPAVNRPSDPPREESPQGSMDDLAARVVV
                     HAPEPISLPLSPDAMSFAAGTPSTLPPPPPANAFLPEDLRVPWSWLHLLLFGLFAFGS
                     ILVIQIALVVYFNASRHLSPKEIEQLFQNRPDVAVGSNVLWFFLIFLFLYVTIAALQG
                     RPFWPTFGWKKLGANPDWPVSPWVYLAGGAGLAVCVAIASTKIKTPDHLPIQDLFKNR
                     TGAFLLMGMAVLIAPLVEETVFRGYLYPLFASSCARIARHFGFDPASAVQTGTRAGIV
                     VTGVLFGFLHGAQLGWTWALVGVLSIVGIVFTFVRARARSVFASYLLHLGYNSFIAFS
                     AIFSTRFFTQMPPHP"
     gene            complement(2934..3554)
                     /locus_tag="VFR42_07465"
     CDS             complement(2934..3554)
                     /locus_tag="VFR42_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369029.1"
                     /translation="MNRIIRTIAVAFGTLVVAGMMAVGARAQCGASDGHLASNSATAL
                     LMHSGAKTAAASVKANAIAQSVAPGGAQVVGFWHVTFISKGTGFIPDGTVVDQGFSQW
                     HSDGTEILNSSRPPATSNFCLGVWEKTGPSAYKLNHFALSSDLNGNMIGPANIRENVT
                     LDSHGATYSGTFSIDQYDTSGNLLAHIVGEVKATRITVDTKVSDIL"
     gene            3792..>5296
                     /locus_tag="VFR42_07470"
     CDS             3792..>5296
                     /locus_tag="VFR42_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0369030.1"
                     /translation="MTPVLLRFADFELDSLRYELRRNGRPLKLEKIPMELLQLLAESD
                     GRLVTREEIEARLWGQHVFVDAEHGINTAVRKIRQVLGDDPDQPRFVQTVQRKGYRFV
                     AEVRREMAGSSALPAVSHEPVRTAPLQAPASVGEFRSERPRADQTTFWSKRAWLSVSA
                     GVVLLAAWPTYRLAVSRLATAASAPPMIRSIAVLPLDNLSGDASQEYFADGMTDELIT
                     MLAKYRSLRVISRTSAMQYKKTKKSLPEIARELSVDGIVEGSISRSHDKLRVTAQLVY
                     GPTDTHLWAESYSRELSDALLLQHDLAKDIAARVNAASGGAAASVRRPAGNAAARDAY
                     FHGRYEWFSQHYPKSRELFQEAIRLDPKYAAAYSGLADSYTASVASGLESPAEVRPLA
                     EAAAQKALELDDTAPEAHHAMAAVSYFFRWDWPAAERECLRTIELNPSLAETHHLYAY
                     VLGTEGRTEESLQQDKIAWELDPFARPWAYGYGLTRARRFDEAIQELRQRAA"
BASE COUNT         1012 a         1761 c         1376 g         1147 t
ORIGIN      
        1 gcattggccc gcgttctgca cgtcgccaac ggtaactcca acggctcact caggtaatct
       61 cgtcgcagga gaaccccgcg tatgggcgct attctctatg tcggcatcaa acttttcgcc
      121 tacatggctt ggatgtattt aggattacgc accttgcgca gcggcgccaa gtcttctatg
      181 tggtgcgcga ttttcctggg catcctcaga ctgtttctcg gtttgacctt cgcccttgtc
      241 atctatttcc tcagctccgc ggtgctttcc cgccttggtt ccggcctcgc gccaaatgtt
      301 gtcacctatc ttgccgtata tgtgccggtg cgctggctcg aatggagcat gatggctgct
      361 cttctcatgt ctccttcgct ctcgcaattt atcctcggca ctgcaaggac cgaccgcctc
      421 tggcgcctcg gcggcatcgc catctcctgc ctcgccgaca tccctcttat cgtttctcta
      481 ggcggcgtca tccccaccgg ccgcttcctc tgctgagcgc agccgaagcc gctctcttgc
      541 cactgcatca accacttgtc attccgaccg aagtgctccg gcgaagccgg ccgcgaaagg
      601 gcggacctcg gctcaatttc accacctcag ggcgatagct tcggaagcgc cgctttcttg
      661 cctaatccgc tgcccgcgtt tacagtgaac tgtccccaac gcaatgacca agtccgccta
      721 cactacccag cccatcgccc tcggcgaagt cgccacctat ccgctcacct cccgcaaaag
      781 caaagtctcc atcgccgatt tcgccaagcc cacaacaaaa tcaaatgtat cactcagaaa
      841 ttttctcgac tccctcccca acatcctggc cgcacaagat ctccgctcac tactttccgc
      901 tatccacgca gctaagcgct ctcgcaagcc catcctctgg ggcctcggcg gccacgtcat
      961 caagaccgga ctcggcccca ccctggccga ccttatgcac cgcggcttgg tctccggcat
     1021 cgctatgaac ggcgccgccc tcatccacga tttcgaaatc gcgcttagcg gtggcacttc
     1081 cgaagacgtt gacgccggcc tctgcgaagg ccagttcggc atggccaccg aaaccggttt
     1141 ctacctcaat caaatcgcct ccaccgccaa tttttccgcc atcggctatg gcgaagcggc
     1201 cggtaaattt ctcgccgcgc aaacgcgccg cctcgctccg caaaacgccg ccgccagcat
     1261 tctctacaac gcctatcggc acaaaattcc cgtcaccatc catctcgcca tcggtaccga
     1321 cattccgcac atgcatcccg ccgccgatgg cgcatccctc ggcgcggcca ctcatcacga
     1381 cttccgcctc ttctgttcgc tcgtcaaagc catgcacccc ggcggcgtct atctcaactg
     1441 gggctccgcc gttcttcttc ccgaagtttt tcttaaggcc gtttccgttg tgcgcaatct
     1501 cggcgttcct ctccgcccca tcactacggc aaacttcgac tttatccagc actaccgtcc
     1561 attgcagaat gtagtgaagc gtcccaccgc cccggcagcc gggcggactt ccgcctcaaa
     1621 ggggtatgcc atcaccggtc atcacgaact cttactgcct ctcctagccg cggctctcgc
     1681 ttcgaattgg ccacgcgccg gcgctagtgc aaaacgccag tccaccaaaa aggctccccg
     1741 ctcgaaatga atcccttcga cgagaaacct ccgcactcca gtccggacga tcagcctgcc
     1801 gtgaatcgac cttccgatcc tccccgcgaa gaatcgcccc aaggatcgat ggacgacctt
     1861 gccgcgcgcg tcgttgtcca cgcgcccgaa cccataagcc taccgctctc tcccgacgca
     1921 atgtcgttcg ctgccggtac gccctcgacg ctcccgcctc ctccgccggc caatgctttc
     1981 ctgccggaag atctccgcgt cccctggtcc tggctgcacc tgctactttt cggcctattc
     2041 gccttcggca gcatcttggt gattcaaatc gcactggtcg tctactttaa cgccagccgt
     2101 cacctcagcc ccaaagaaat cgagcaactt tttcaaaacc ggcccgatgt cgccgttggc
     2161 tccaacgtac tctggttttt tctcatcttt ctttttctat acgtgaccat cgccgccttg
     2221 caaggccggc ctttctggcc caccttcggc tggaaaaaac tgggcgccaa tcccgactgg
     2281 cccgtcagcc cctgggtcta tctcgcgggc ggcgccgggc tcgctgtgtg tgtcgccatt
     2341 gccagcacca aaatcaagac gcccgatcat ctcccgattc aggatctttt caagaatcgc
     2401 accggcgcat ttctcctcat gggcatggcc gttctcatcg ctccgctggt ggaagagacc
     2461 gtctttcgcg gatacctcta tccgcttttc gcgtcctctt gcgctcgcat tgcccgccat
     2521 ttcggtttcg atcccgcttc tgccgtgcaa accggtacgc gcgccggcat cgtcgtcact
     2581 ggcgtgctct tcggttttct ccatggagcg cagctcggct ggacctgggc tctggtcggc
     2641 gttctctcga tcgtcggcat cgtcttcact ttcgttcgcg cccgcgctcg ctccgtcttc
     2701 gccagctacc ttctccacct cggctacaac tctttcatcg ctttttcagc tatcttctcc
     2761 acccgctttt tcactcaaat gcccccgcac ccctgaaact ttcttgcagg cgaacgccgg
     2821 cagcgcaata cctagaaaga cataaagaaa ccggatggct gtcggggagc gcagccatcc
     2881 tccccggacc tataaagcag cggcagctta cagagccctg ccgctgctga gccttacagt
     2941 atgtccgaga ccttggtgtc cacagtgatg cgagtcgctt tcacttcccc cacgatgtgc
     3001 gcgagcaggt tgcccgacgt atcgtactgg tcgatcgaaa atgttccgga gtaagtcgct
     3061 ccgtgagaat caagagtcac gttttcgcga atgttcgctg ggccaatcat gttgccgttc
     3121 aggtccgaac tcagcgcaaa gtgattcagc ttgtaagcgg aaggtcccgt cttttcccag
     3181 acgcccaggc agaaattgct ggttgccggt ggccgcgaag agttcagaat ctccgtcccg
     3241 tccgagtgcc attgtgaaaa gccctggtcc actacggtgc catccggaat gaaccctgtg
     3301 ccttttgaaa tgaaagtgac atgccagaag ccgacaacct gtgcgccccc tggtgcgaca
     3361 gattgagcga tggcattcgc cttgacgctg gccgccgccg tcttcgcccc cgaatgcatc
     3421 agcagcgcgg tggcagagtt ggacgcgagg tgtccatccg acgccccgca ctgcgccctt
     3481 gcgccgaccg ccatcatgcc cgcaaccact aacgtgccaa acgctactgc aatcgttcta
     3541 attatcctgt tcataggatc tccccttacg gctggaaaca ttgtcgagac tctgaatctg
     3601 gtttacttgc gctcccgagc tggttcgccg ccggggcaaa ctagccactg acgccgtgtt
     3661 acgacaatgg atagcttgtg cagcagcggt aaagatttgg tgaaggccta aggggctgaa
     3721 aacaaaggtg ttctttaacc catacctacg cgggtgctat actcccctaa aagtccccct
     3781 gcagaaacgc cgtgactcca gtccttctca gattcgccga tttcgagctc gactccctgc
     3841 gttacgagct tcgccgcaac ggacgtcccc tcaaacttga aaaaattccc atggagcttt
     3901 tgcaactgct tgcggaatcg gacggacggc tggttacccg cgaagagatt gaagcgcgcc
     3961 tgtggggcca acacgtgttt gtagacgccg aacacgggat taatacggcc gtgcgcaaga
     4021 tccggcaagt gctcggcgac gaccccgacc agccacggtt cgtccaaacg gtgcaacgca
     4081 agggataccg gttcgtcgcg gaagtccgcc gggagatggc aggctcatcc gcgctgccgg
     4141 cggtttcaca cgaaccggta cgcacagcac ctctgcaggc gcctgcgagc gtcggggagt
     4201 ttcgatcgga aagaccgcgc gcggaccaaa ctactttttg gagcaaaagg gcttggctgt
     4261 ccgttagcgc gggtgtggtc ttgctggcgg cctggcccac ttaccggctg gcggtttcgc
     4321 gtttagccac tgccgcctcc gcacctccga tgatccgctc gattgcagtg ctgcctctgg
     4381 acaatctttc cggcgacgct tcgcaggaat actttgccga cggcatgacc gatgagctga
     4441 tcaccatgct agcaaaatat agatcactgc gggtgatctc gcgcacgtcg gcaatgcagt
     4501 acaagaaaac gaaaaagagc ttgccggaga tcgcgcgaga gctttcggtg gacgggatcg
     4561 tggagggttc gatttcacgt tcacacgaca agttacgggt gacggcgcaa ctggtatacg
     4621 gtccgacgga tacgcatttg tgggcggaaa gttacagccg cgaattaagc gacgcactgc
     4681 tgttgcagca cgatctggca aaggacatcg cggcgcgggt gaacgcagct tcgggcggag
     4741 ctgcggcgag cgttcgccgc cctgccggga atgccgcggc acgggacgcg tacttccatg
     4801 gcagatatga atggttttcc cagcactatc ccaaaagtcg ggaacttttt caggaagcga
     4861 tccggctcga tcctaagtat gcggccgcat acagtggact ggcggacagt tacactgcct
     4921 cagtggcatc gggattagag tcgcctgcgg aagtaaggcc gctggccgaa gccgcggcgc
     4981 aaaaagcgct ggagttggac gacacggcac cggaagcaca tcacgcaatg gccgctgtga
     5041 gttatttctt tcgctgggat tggccggcgg cggaacgaga gtgcctgcga acgatcgaat
     5101 tgaatccaag cctggcggaa acccatcacc tctacgctta tgtcctgggg accgaggggc
     5161 gcaccgaaga atcgctgcag caggacaaga ttgcgtggga actcgatccg ttcgcgcggc
     5221 catgggcgta cggctacggc ttgacgcgtg cccggaggtt cgacgaagcg atccaggaac
     5281 tgcggcagcg agcggc
//
LOCUS       DASTPM010000425         3206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_563166, whole genome shotgun sequence.
ACCESSION   DASTPM010000425 DASTPM010000000
VERSION     DASTPM010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563166"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1256)
                     /locus_tag="VFR42_07475"
     CDS             complement(135..1256)
                     /locus_tag="VFR42_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU0369031.1"
                     /translation="MAGITLSELLKKMIEMGGSDLHITTSSAPRVRVHGKLRPLDMPP
                     LTAADTKSLAYSVLTDAQKHRFEENLELDFSFGLKNLARFRGNVFNQRGAVGAVFRTI
                     PWEIKGFDALGLPPVAKTLCDKPRGLILVTGPTGSGKSTTLAAMLDKINSEREEHMIT
                     IEDPIEFLHNHKKCLVNQREVHADTHSFSNSLRAALREDPDVVLIGEMRDLETIESAL
                     RIAETGHLTFATLHTNSAASTINRIIDVFPAHQQPQIRAQLSMVLEGVLCQALLPRVD
                     GRGRAMVMEILVPNPAIRNLIREDKIHQIYSAMQTGTGQSGMQTFNQSLANTYFAKQI
                     SLETALSRSSNPDELQDMINRGVSSGPAGSQRVPVGLKR"
     gene            complement(1271..2983)
                     /gene="pilB"
                     /locus_tag="VFR42_07480"
     CDS             complement(1271..2983)
                     /gene="pilB"
                     /locus_tag="VFR42_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEU0369032.1"
                     /translation="MSVRLGEILVKESLITQDQLQKALEFQRVNGGKLGSCLTKMGFI
                     TDDDITGVLSRQYGVPSINLKYYEIDPNVIRLIPQDTATRYQVVPLSRVGSVLTIAMT
                     DPTNVFAMDDVKFMTGFNVEPVVASEAAIADAISRFYGGGSSNGEELTNLMRDLVEEE
                     LELAPDDAELDAQALEKAAEEAPIIKLVNLILTDSVKRGASDIHIEPYETEMRVRFRI
                     DGMLQTVMTPPLKVKDAMTSRMKIMAKLDIAEKRLPQDGRIMIKYKADGKKKELDFRV
                     STVPTLYGEKIVLRLLDKENLRLDMTKLGFEQSSLTKFERNILKPYGMVLVTGPTGSG
                     KTNTLYSSVARLNQVDTNIMTAEDPVEFQLGGINQVQMKEQIGLNFAAALRAFLRQDP
                     NIILVGEIRDFETAEIAVKAALTGHLVLSTLHTNDAPSTISRLMNMGIEPFLVATSVN
                     LICAQRLVRRICSNCKEEVEVPPQVLLDAGYTEDELKTTKIYHGKGCSICNKGGYKGR
                     TGLYEVMEINDELRELILVGASALELKKKAVEQGMLTLRRSGLVKIAAGMTTMDEVLR
                     ETVL"
BASE COUNT          612 a         1056 c          790 g          748 t
ORIGIN      
        1 cagctccagt ttgttcgtcg ccgtcaattc gccactgacg gcggctcccg aacgattcgt
       61 tcctcgatat gtatagaccg gcatgtagcc ctcctcggtc gcctaaactc cagaattctt
      121 cgtccccgaa tttcctaccg cttcaatccc accggcacgc gctgcgaccc cgccggccca
      181 gacgacactc cacgattgat catgtcctgc aactcgtccg gattcgacga acgcgacagc
      241 gccgtctcta ggctgatttg cttcgcaaaa taagtgttag ccagactctg attgaacgtt
      301 tgcatgcccg actggcccgt cccggtctgc atggccgaat aaatctggtg aatcttgtct
      361 tcgcggatca ggttgcggat cgccgggttc ggcaccagaa tttccatcac catcgcccgt
      421 ccgcgaccat ccaccctggg caacagcgct tggcaaagca cgccttccag caccatcgaa
      481 agctgtgcgc ggatctgcgg ctgctggtgc gctggaaaaa cgtcgataat acggttgatc
      541 gtcgacgccg ccgagttcgt gtgcagcgtc gcgaacgtca agtgaccggt ttccgcgatg
      601 cgcaacgccg actcaatggt ctccaggtcg cgcatttcgc cgatcagcac cacgtctgga
      661 tcttcgcgca acgccgcgcg cagcgaattc gagaaggaat gcgtgtccgc gtgtacttcg
      721 cgctggttga ccaggcactt cttgtggttg tgcagaaatt cgatcgggtc ctcgatcgtg
      781 atcatgtgct cttcgcgttc gctgttgatc ttgtccagca tggcggccag cgtcgtcgac
      841 ttacctgaac ccgtcggccc ggtcaccagg atcaatccgc gaggcttgtc gcaaagcgtc
      901 ttcgctaccg gcggcaatcc gagagcatca aaacccttga tttcccacgg aatggtacgg
      961 aacaccgcac cgaccgctcc gcgttggtta aagacgttgc cgcggaaacg cgccaggttc
     1021 ttcaacccga aggaaaagtc tagctccagg ttctcttcaa accggtgctt ctgcgcgtcc
     1081 gtcaacacgc tataggccag cgacttggta tccgcggctg tcagcggcgg catgtccagc
     1141 gggcgcagct tgccatgcac gcgcacccgc ggcgcgctgc tcgtggtgat gtgcaagtcg
     1201 ctcccgccca tctcgatcat ttttttgagc aattcgctaa gcgtaattcc ggccatcgtc
     1261 gcaccctttc ctacaaaacc gtctcgcgca atacttcgtc catggtggtc attccggcgg
     1321 cgatcttcac cagaccgctg cgccgcaaag tcagcattcc ctgctccaca gcctttttct
     1381 tcaactccag cgccgacgcg cccactaata tcaactcgcg caactcgtcg ttgatctcca
     1441 tcacttcgta caaacccgtg cgacccttgt acccgccttt gttgcaaatg ctgcagccct
     1501 tgccgtgata aatcttagtc gtcttcagct catcttcggt gtaacccgcg tccaacagca
     1561 cctgcggcgg cacttccact tcctccttgc agttcgaaca gatgcgtcgc accagccgct
     1621 gcgcgcagat caggttcacc gaagtggcca ccaggaacgg ctcaatcccc atattcatca
     1681 aacggcttat ggtggacggt gcatcgttgg tgtgtagggt cgacagcacc aagtggcccg
     1741 tcagtgccgc ctttaccgca atttccgcgg tctcaaagtc gcggatttcg ccgaccagaa
     1801 taatgttcgg gtcttgccgc aaaaacgcgc gcaacgccgc ggcaaagttc aacccgatct
     1861 gctctttcat ctgcacctgg ttgataccgc ccagttggaa ctcgaccgga tcttccgcgg
     1921 tcataatgtt cgtatcgacc tggttcagcc gcgccaccga cgaatacagc gtgttggtct
     1981 tgcctgaacc cgtcggcccg gtcactagca ccatcccgta cggcttcaaa atgttccgct
     2041 caaacttggt cagcgacgat tgctcgaatc cgagcttggt catgtccagc cgcaggttct
     2101 ctttgtccag caaccggagt acgatttttt cgccatacag cgtcggcacg gtcgaaacgc
     2161 ggaagtccag ttccttcttc ttcccgtccg ccttgtactt gatcatgatg cggccgtctt
     2221 gcggcagacg cttttccgca atgtccagct ttgccatgat cttcatgcgg ctggtcatcg
     2281 catctttgac cttcaacggc ggtgtcatca ccgtttgcag catgccgtca atgcggaacc
     2341 gtacccgcat ctctgtttcg taaggctcga tgtgaatatc gctcgcgccc cgcttcactg
     2401 aatccgtcaa aatcaggttt accagcttga tgatcggcgc ttcttccgcc gctttttcca
     2461 gcgcctgcgc atccaactcc gcatcatccg gcgccagttc aagctcttcc tccaccaaat
     2521 cccgcatcag gttggtgagt tcttcgccgt ttgacgagcc gccgccgtaa aaacgcgaaa
     2581 tcgcgtccgc aatggccgct tccgacgcta ccaccggctc cacgttgaat ccggtcatga
     2641 acttcacgtc gtccatcgcg aacacgtttg tcgggtcggt catcgcgatg gtcagcaccg
     2701 agcccacacg cgacaacggc accacctggt agcgcgtcgc cgtatcctgc ggaatcagcc
     2761 ggatcacatt cggatcaatt tcgtaatatt tcaggttgat ggaggggacg ccgtactgcc
     2821 gcgacagcac gcccgtgatg tcgtcatccg tgatgaatcc catcttcgtc aggcagcttc
     2881 ccagcttgcc gccgttcact cgttggaact cgagcgcctt ttgcagctgg tcctgcgtga
     2941 tgagactttc tttgacgagg atttcgccca aacgaaccga catccgcacc tgctccgtac
     3001 agccgccgcg ccaaaccatt gcctcgtggc gctgctctgc ggccgcaaca ctcctcgcta
     3061 aacctaacaa aacttgtcac actcggatgt tcgattttcg tccctcgttc tgaactcgcc
     3121 actccctgtc cgccggtaca gtctctttaa aaccctgtaa aactgcctct cgcacttgca
     3181 atctgcaaaa ccttttttgc gagcgg
//
LOCUS       DASTPM010000426         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1255000, whole genome shotgun sequence.
ACCESSION   DASTPM010000426 DASTPM010000000
VERSION     DASTPM010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255000"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..3252)
                     /locus_tag="VFR42_07485"
     CDS             complement(25..3252)
                     /locus_tag="VFR42_07485"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369033.1"
                     /translation="MTVPRPAPPLRLYLLFAATILFVGLLAFAPRTRAQEISPDAYAG
                     LRWRFLGTHRGGRITTVAGVVGQPNLYYAGTPNGGVWKTDDGGRTWKPIFDSVPVPSV
                     GAMAVAPSNPKVIYVATGEQGKGAGIFKSIDGGENWASAGLADNTLFDGLIVDPENPD
                     VVIAGASGRGREEPRGLYKTTDGGKTWKKTFLETDVNAGVADMTAAADNPKILYAALY
                     PISAPPPEEKGTAAEARVLVSQDEGSTWTVTAGAGLPEKGRGRIGVAVVPGTAGKSVL
                     AILNQGLFRSDDSGATWHKSTNDPRILGSGYFSKVYVDPNQPNVVYVMQTCSYRSNDG
                     GKTFFAFRGAPSGEDHHVIWIAPDHSNRMILGTDQGAIISINGGATWTDWLNQATGQL
                     YHVITDNQFPYHAYAAQQDSGTIVVPNRSDYGQITYRDWFSSGGFESGYIAPDPLNAN
                     LVYSVGWFGTVLRLDRVTGQIATVYVPPADYKTVWETPLVYSPRDAHTLYYGSQYLLK
                     TTDGAVSWSVVSPDLSNSPQHPAEVKKPGGGHQPDPDAEEEDPAAEADKDAAQFARTG
                     AIHTIAPSPREDGMIWVGTSNGLIQLFRAHAWHDVTPTDLPKGSDVAMVEASRHDANT
                     AFTVVKVRRDDHPYIFRTHDAGKTWTKIVTGLPEGVPADGVREDVQRKGLMFAGTERA
                     AYVSFDDGDHWQTLQLNLPPSPVTDFAVHGDDLVASTFGRGLWILDDITPLRELTAKA
                     SLEPAHFFKPQTALRIRWDNHEETPLSPEFPASPNPPDGAILDYFLKNSPRGEVTLDL
                     LDAKGNHIRHYSSNQPAASPLVGNAADVWFAPRATLQTSPGLHRFVWDLRADDPLTLT
                     YGYFGGKLDYIEYTLPDHSVPGSTPRQQPPGPLVPPGVYTAILKVDGKEYRQPLEVDL
                     DPRVHTPPADLQEQWNLAAGIASAMSASYAAYNDFAALQAAIHERQSALAGKDQAKAL
                     AESLQSLEKSAMNFAEGSDDTQGIGPINRDLSRYLVMIESADLKPAASARQAVQKACT
                     ALQTNLHAWQKLNSEDVPAASKLLESVPLPALPVAPTVPELACSP"
BASE COUNT          648 a          969 c         1108 g          703 t
ORIGIN      
        1 actcggtagg ccgcgtcggc tcgctcaagg cgagcaggca agctcaggca cagtgggagc
       61 gactggaagc gcagggagag gaacgctttc taagagtttg ctcgccgctg gtacatcttc
      121 cgagttcaat ttctgccagg cgtgcaggtt tgtctgtaga gcggtgcagg ccttttgaac
      181 ggcctggcgc gccgaggcgg cgggcttcag gtcggcgctt tcgatcatta ccagatagcg
      241 cgagaggtca cggttgatgg gcccgatgcc ctgggtgtcg tcggaaccct cggcaaagtt
      301 cattgcggac ttttcgagcg actggagcga ttcggctagg gcttttgcct ggtctttgcc
      361 tgccagcgcg gactggcgct cgtgaattgc cgcctggagc gctgcgaagt cgttgtaggc
      421 ggcgtagcta gcggacattg cggaggcaat gcctgccgcg agattccatt gttcttgcaa
      481 atcggctggc ggcgtgtgca cgcgagggtc gaggtcaact tcgagtggtt ggcggtactc
      541 cttgccgtcc actttaagaa tggcggtata taccccgggg ggaaccagtg gtcctggcgg
      601 ctgctggcgc ggagtcgagc cgggaacgga gtgatcaggc agggtgtatt cgatgtagtc
      661 aagcttgccg ccgaagtagc cgtaggtgag cgtgagcgga tcgtcggccc gcaaatccca
      721 cacgaagcgg tgaaggccgg gggaggtttg cagtgttgct cgaggagcga accaaacgtc
      781 cgccgcgttg cccacaagcg gcgacgccgc cggctggttg ctggaatagt ggcggatgtg
      841 attccctttc gcgtctagga gatccaaagt aacttcgcct cgcggagaat ttttcaagaa
      901 atagtccagg atggcgccgt cgggaggatt aggcgacgcg gggaattccg gcgacaacgg
      961 ggtctcttcg tgattgtccc agcggatgcg cagggcggtt tgcggcttga agaaatgtgc
     1021 tggttccaag gaggctttgg ccgtgagctc gcgcaagggc gtgatgtcgt cgagtatcca
     1081 caaaccgcgg ccgaaggtgg aggcgacgag gtcgtcgccg tggacggcaa aatcggtgac
     1141 cgggctgggg ggaagattca gctgcagcgt ttgccagtgg tcgccgtcgt cgaacgaaac
     1201 ataagcggct ctttcggtgc cagcgaacat caagccttta cgctggacgt cttcgcgaac
     1261 gccgtcggcg ggcactcctt cgggcagacc ggtcacaatt ttggtccaag tcttgccagc
     1321 gtcgtgggtg cggaagatgt agggatgatc gtcgcggcga actttgacca cggtgaacgc
     1381 tgtgttggcg tcatgcctag acgcttcgac catggcgacg tcgctgccct tcggcagatc
     1441 ggttggcgtt acgtcgtgcc atgcatgggc acgaaaaagc tggatcaagc cgttcgacgt
     1501 gcccacccag atcatgccat cctcgcgcgg cgagggagca atcgtgtgaa tggctccggt
     1561 gcgcgcgaat tgtgccgcgt ccttgtcagc ttcggcagcg ggatcttcct cttcggcatc
     1621 agggtcgggc tggtggccac caccgggctt tttgacctca gccggatgtt gcggcgagtt
     1681 gcttaggtct gggctgacca cgctccagct gaccgcgcca tcggtagtct tcagcaagta
     1741 ttgcgagccg tagtaaagcg tgtgcgcgtc gcgcggcgaa tacactaacg gtgtctccca
     1801 gacggtcttg tagtcggccg gcggaacata aaccgtggca atttggccgg tgacgcgatc
     1861 gaggcgcaac accgtgccaa accacccgac ggagtacacg agattcgcgt tcagcggatc
     1921 gggtgcgatg tagccgcttt caaacccgcc gctggaaaac cagtcgcgat aggtgatctg
     1981 cccatagtcg ctgcgattcg gcacgacgat agtgccacta tcctgctgcg cagcgtacgc
     2041 gtgatacgga aactgattgt cggtgatgac atgatacagc tggccggtcg cctgattgag
     2101 ccaatcggtc caggtcgctc cgccatttat gctgatgatc gcgccctggt cggtgccgag
     2161 aatcatacgg ttggaatggt caggagcgat ccagatgacg tgatgatctt cgccgctagg
     2221 cgctccgcga aacgcgaaaa aagtcttgcc gccgtcgttg gagcgataac tgcacgtctg
     2281 catcacgtag acgacgtttg gctgattggg gtccacgtaa accttgctga aataaccgct
     2341 gcccaggatg cgcggatcat tcgtggactt gtgccacgtg gcgccggaat cgtccgaacg
     2401 aaagagccct tgattcagta tggccagcac gctcttgccc gcggtgcccg gaacgactgc
     2461 aacaccgatc cgtcctcgcc ccttctccgg cagaccggct cccgccgtca cggtccacgt
     2521 agatccttca tcttgcgaaa ccaggacgcg ggcttctgca gcagtgccct tttcttccgg
     2581 tggcggcgcg cttatcggat aaagcgcagc gtagagaatt tttggattgt ccgcggctgc
     2641 ggtcatgtcg gcgactcccg cgtttacatc tgtctccagg aaggttttct tccaggtctt
     2701 gccgccgtcc gtcgtcttgt acagcccgcg cggctcttcg cgccctctgc ccgaggctcc
     2761 cgcgatcacc acatccggat tctctggatc cacaatcagg ccatcgaaca gcgtgttatc
     2821 cgccagaccc gcgctggccc agttttcgcc gccgtcgatg gatttaaaaa ttccggcgcc
     2881 ttttccctgt tcgcccgtgg cgacataaat gactttcgga ttggatggcg cgacggccat
     2941 cgcgcctacg gatggcactg gaacggaatc aaaaatcggc ttccaggtgc ggccaccgtc
     3001 atcggttttc cagacgcctc cgttcggcgt gcccgcgtaa tagagattcg gctggccgac
     3061 gactccggcg acggtggtaa tgcgtccacc gcgatgcgtg cccagaaagc gccaacgcag
     3121 cccggcgtaa gcatcaggcg agatttcctg agcgcgggtt cgcggcgcga aggcgagcag
     3181 tccaacaaag agaatggtgg cagcgaaaag cagatacaga cgcagcggcg gggcggggcg
     3241 ggggacagtc acggtgtatt ttctccaggc tcagaaagtg tactacggca cgcgcggcgg
     3301 agtcgcatca tcgccggtga ttagtcgcgc accgcggctg aaagtcagat actcgaatcc
     3361 ccattgaatg aacaccagca cgcggctctg gaactccacg atgtacatca ggtggatgaa
     3421 gagccaca
//
LOCUS       DASTPM010000427         5473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_591669, whole genome shotgun sequence.
ACCESSION   DASTPM010000427 DASTPM010000000
VERSION     DASTPM010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591669"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFR42_07490"
     CDS             <1..618
                     /locus_tag="VFR42_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU0369034.1"
                     /translation="RKTAASLPRHDVPERTESGYTEEGNASWYGPPFHGRRSSNGEVY
                     DMNKLTAAHRTMAFNTMVRVTNLTNGKTVVVRITDRGPFIENRIIDLSKAAAQEIESI
                     GPGVVPVRLEVVSGNDPFGGYFTVQVGAFKERGNAERLRDRLDTLYSPATIQQVALDD
                     GMFYRVRVGKVSGEQAAQRFADDLKLREGFHAMVIRLDDDAGGAN"
     gene            618..959
                     /locus_tag="VFR42_07495"
     CDS             618..959
                     /locus_tag="VFR42_07495"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEU0369035.1"
                     /translation="MSADCLFCKIAAKQIPAKLVHEDADVFAFEDINPQAPTHVLVCP
                     KKHFPSLEHATPEEEAALGKVALVAAKIAKDRKLGSYRTVINTGSGAGQTVFHVHLHL
                     LGGRAFSWPPG"
     gene            1051..3297
                     /locus_tag="VFR42_07500"
     CDS             1051..3297
                     /locus_tag="VFR42_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0369036.1"
                     /translation="MIRRAAWKRELHVLAVGLTTLIAKNSQAQTTALPAPDQLTVKRI
                     YSQPSLSGRPYRGVQWSPDSKTVSFFETKGQGKDARTELWGMDVATGARRLLLSSENL
                     ETALPPDKSQPTQATGLGRRPASQYFWSPDGASILFIGGKSLGLLDVKTQQARMLLAG
                     QLAITDVKFSPDGKQVSYVSGHNLHLISLNEGNDRTLTTGGTEEVRKGELDWVYPEEL
                     DLKTAYWWSPDSTKIAFLQMDERKVSKYPLVDFASYEGEAEEEHYPVAGGANPVVQVY
                     TVEIAGGKTKLMDTGTETNQYIPRVDWLQDSKRVAIQRLNRAQTQLDLLVADASTGKS
                     SVLLSEKDAYWINVSDDLRFFKDGKRFLWSSERSGYRHLYLYGLDGKEIAQLTNGEWE
                     VSAVQAVDESKGLVYFTATEKTPSERHLYRVAFDRTGFSRITQKDGTHATLFSPDVST
                     FVDTYSNTLTPQQQWVSKSDGSSPLVLNENQVAELEKYHLTAPEFLKVKSHDGIELNA
                     WIIKPANFDPAKKYPVLVYTYGGPHAQVVTNAWGGPTFLWHQLMAQKGVIIFALDNRG
                     SAGRGHLFEEPIHLRFGAQELSDQHDGVTWLKVQPWVDGSRIGIWGWSYGGHMTLHAM
                     FEDPEDFSAGFAGGPVTDWHYYDSIYTERYMGLLPENEGNYKSSSPVEKAEQLRGKLL
                     IAHGTGDDNVHFANTLSLIDKLIEKGKYVEVLPFPGRGHGVSDPPARILLMNRVTKFF
                     LENLGEVH"
     gene            3401..>5473
                     /locus_tag="VFR42_07505"
     CDS             3401..>5473
                     /locus_tag="VFR42_07505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0369037.1"
                     /translation="MSLSKILRFGVLLLLFVSAMGQALAQSPARKLPMIPFEKYKLKN
                     GLEVILSEDHTLPLVSVNIWYHVGPANEKAGRTGFAHLFEHMMFEGSQHVGSKAHIAY
                     LEGAGATDINGTTNFDRTNYYETVPSNQLELALWLESDRMGYLLGTLDIEKLANQRDV
                     VRNERRQSVENAPYGLAEEALVHLSLPKNHPYYAEVIGSHADVEAAKLEDVREFFQQY
                     YTPNNATMAVVGDFDKSAIKGLVEKYFGSIPAGPAVPKIDVTTPPITSERRATVTDQV
                     ELPRVYMGWITPPVFSAGDAECNFYSQILGAGKSSRLYKSLVYEKQIAQDVSTSMEET
                     KLGSLFELVVTAKPGVKPEDLEKAIDDVINKLAADGPTAAEVERARNITETALVRGLQ
                     RTNGVANRLNYYNQFLGTADYFAKDIARFDAVKPADVQLVAQTFFKPDARSVVFAVPG
                     KKVIEDVPKTSEEENKKQAADAGPSKVTMPDEAWRANAPKPGPAPKFTLPMPEQAKLS
                     NGLTILLVERHNLPFVSATVYTMSGSELNPLDKPGLSSFVAGMLMEGTTNRSPLKFAE
                     DADQIGASIDSSAGYSSTTVSLSALSWNAGAALDLASDAILHPAFAEKEVERIRNQRL
                     TAVLQENDEPFALAQRTASHLVFPNSPYGFSAIGTEASNKAIMRTDLTGFWQKAYVPS
                     NSVLAISGD"
BASE COUNT         1290 a         1561 c         1537 g         1085 t
ORIGIN      
        1 cgcaaaaccg ctgcaagctt gccacgccac gacgtgccgg agcggaccga gagcggctat
       61 acggaagaag gcaacgcctc gtggtatggt ccgccgttcc atggacgacg atcttcaaac
      121 ggcgaagtct acgacatgaa caagctgacg gcggcgcacc gcacgatggc attcaatacc
      181 atggtgcgcg tgacgaacct gaccaatgga aaaaccgtcg tcgtgcggat caccgaccgc
      241 ggcccgttta tagagaatcg gatcattgat ttatcgaagg cagcagcaca agagattgag
      301 tccatcggac cgggcgtcgt gcctgtgcgg ctggaagtcg tctcgggaaa cgatccgttc
      361 ggcgggtatt ttacggtgca ggtgggcgcg tttaaggaac gcggaaacgc cgaacggctc
      421 cgcgaccgcc tggacacgct ctattcaccg gcgacgatcc agcaggtcgc tctggatgac
      481 ggaatgttct atcgcgtaag ggtgggcaaa gtttctggcg agcaggcggc acaaagattc
      541 gccgacgatt tgaaattacg agagggattt cacgcgatgg tcatccggct ggatgacgat
      601 gcgggaggag cgaattaatg agcgccgatt gtttgttctg caaaattgcg gcgaagcaga
      661 ttcccgccaa gctggtgcat gaagatgcgg acgtgtttgc gttcgaagac atcaacccgc
      721 aagcgcctac ccacgtgctg gtctgcccga agaagcattt cccgtcgctg gagcacgcta
      781 cgcccgaaga ggaagccgcg ctgggcaaag tggcgctagt cgcggcaaaa attgcgaaag
      841 atcgcaaact gggaagttat cgaacggtga tcaatacggg aagcggcgca gggcagacgg
      901 tgtttcatgt gcacttgcat ctgttaggcg gcagagcgtt cagttggccg cctggataag
      961 caaacgacca cggactgagt agttcctggc ggcacggatt tcctgaagcg cacccgcgat
     1021 aaaatgactc gccactggag aggcccacgc atgatccgca gagcggcttg gaaaagggaa
     1081 ttgcatgttt tagcggtagg tctaaccaca ctgatcgcca agaactcgca ggcgcaaacg
     1141 accgcgctcc cagcgccgga tcagctcacc gtcaaacgga tctattcgca acccagcctg
     1201 agcggtaggc cctaccgcgg cgtccaatgg tctcccgata gcaagacagt ttcctttttc
     1261 gaaacgaagg gacaaggcaa ggacgccagg acagaacttt ggggcatgga cgtcgccacc
     1321 ggagcgcgac gtctactctt gtcgtcggag aacctggaaa cggcactgcc acccgataag
     1381 tcgcagccaa cccaagccac gggactgggg cgccgcccgg cttcgcaata cttctggtcc
     1441 cccgacgggg cttcgatcct ctttatcggc ggcaaatcgc tgggtttgct ggatgtgaaa
     1501 acacaacaag cccgcatgct gctggctggc caactggcca ttacagacgt gaagttttct
     1561 cctgacggca agcaggtttc ctacgtcagc gggcataatc ttcatttgat cagcttgaat
     1621 gagggcaatg accggacgtt gacgactggt gggaccgagg aagttcgcaa aggcgaactc
     1681 gattgggttt atcccgaaga actcgatctc aagactgcat actggtggtc gccagactcc
     1741 accaaaatcg cctttctgca gatggacgag cgcaaagttt cgaaataccc cttggtggat
     1801 tttgcgtctt atgaaggaga ggccgaagaa gaacactacc cggtcgccgg aggagccaat
     1861 ccggtcgtgc aggtctacac cgtggaaatt gccggcggca aaaccaagct gatggatacg
     1921 ggaacggaga cgaaccaata catcccgcgc gtggattggt tgcaggactc caaacgagtt
     1981 gctatccagc gcctgaatcg cgcacaaacc cagctagacc tgctggttgc cgatgcttcc
     2041 accggaaagt ccagcgtgtt gttaagcgaa aaggacgctt actggattaa cgtgtccgac
     2101 gatttgcgct ttttcaagga cggcaagcga tttctttggt ccagtgaacg cagcggctat
     2161 cggcacttgt atttgtatgg cctggacgga aaggaaatcg cgcagctgac caacggcgaa
     2221 tgggaagtat ccgctgtgca ggcggttgac gaatccaaag ggctggtcta tttcacagcc
     2281 accgagaaaa ctccctccga aaggcatctc tatcgagtcg ctttcgaccg aaccggattt
     2341 tcccgcatta cgcagaaaga cggcacccac gcgacactgt tttcgcccga cgtgtccact
     2401 ttcgtggata catattcgaa cactctcacg ccgcaacagc agtgggtaag caagtcggat
     2461 ggcagctctc ccctggttct gaacgaaaac caggtggcgg agcttgagaa gtatcacctg
     2521 accgccccgg aatttctgaa agtgaaatcg cacgacggaa tcgaattgaa cgcctggatc
     2581 atcaagccgg cgaattttga tccagcgaaa aaatatcccg ttttggttta cacctacggc
     2641 ggcccgcacg ctcaggtcgt gaccaatgcc tggggcggac caacttttct ctggcatcag
     2701 ttgatggccc aaaagggtgt cattattttc gcgctggata atcgcggttc ggccggtcgc
     2761 ggccacttgt tcgaagagcc gattcattta cgcttcggcg cgcaggaatt gtccgatcag
     2821 cacgacgggg taacttggct gaaagttcag ccgtgggtgg atggatcccg tattggtatc
     2881 tggggctgga gttacggcgg acacatgacg cttcacgcca tgttcgagga tccagaggac
     2941 ttcagcgcag gcttcgcagg tggcccggtg accgactggc actactacga ctctatctat
     3001 accgaacgct acatgggcct gctgcccgaa aacgaaggaa attataagtc gtcctctccc
     3061 gtcgaaaaag ccgagcagtt gcgcggcaag ctgttgatcg cgcacggaac cggcgacgac
     3121 aacgtgcatt ttgcaaacac gctctcgttg atcgacaagc tcattgaaaa agggaagtac
     3181 gtcgaagttc tgccattccc tggccgcggc cacggcgttt ccgatccgcc ggcccgcatc
     3241 cttcttatga atcgggttac caagtttttc ctggagaacc ttggcgaagt acactaaaag
     3301 accgcgcggg gcgccgggaa cattccgagg cgcggcatcg ttcaaatctc aagataacca
     3361 ggcaccggct tccgtttgca tgtccgtgga gggaaagatc atgtctctaa gcaagattct
     3421 gcgattcggc gttctgcttc tgctgttcgt ttcggcgatg ggacaggcct tggcacagtc
     3481 gccggctcgc aaactgccca tgatcccgtt cgaaaagtac aaattgaaga acggcctgga
     3541 agtcattctc tcggaggacc acacgctgcc cttggtctcc gtcaacatct ggtatcacgt
     3601 gggcccggcg aacgaaaagg ccggcagaac gggtttcgcg cacctgtttg agcacatgat
     3661 gttcgagggt tcgcagcacg tcggcagcaa ggcgcacatc gcctacctgg aaggtgccgg
     3721 cgcgaccgac atcaacggaa ccacaaactt tgatcgcacc aattactatg aaaccgttcc
     3781 ctccaatcaa ctggagctcg cactgtggct cgaaagcgac cgcatgggct atctgctcgg
     3841 cactctggac atcgagaagt tggccaatca gcgcgacgtt gtgcgaaacg aacgacgcca
     3901 atcggtggaa aacgctcctt acggacttgc agaagaagcc ttggttcacc tgtcgctccc
     3961 gaagaatcat ccctactatg cggaagtgat tggctcgcac gccgacgtcg aagcggcgaa
     4021 actcgaagat gtgcgtgaat tttttcagca atactatacg ccgaacaacg ccacgatggc
     4081 cgtcgtcggc gattttgaca aatccgccat caagggcctg gtcgaaaagt attttggaag
     4141 catcccggcc ggtcccgccg ttccgaagat cgacgttacg accccgccca tcacctccga
     4201 gcgccgcgcc accgtcaccg accaggtcga actcccccgc gtatacatgg gatggatcac
     4261 tcctcctgtt ttcagcgccg gcgatgccga atgcaatttc tattcgcaga ttcttggcgc
     4321 cggcaagagc agccgtctct ataagagtct cgtgtatgaa aagcagattg cccaggatgt
     4381 ttccaccagc atggaagaaa ccaagctggg atctttgttt gaactggtcg tcaccgcgaa
     4441 acccggagtg aagcccgagg atctggaaaa agcgatcgat gacgtgatca ataaacttgc
     4501 cgcggacggc ccgactgccg ccgaagtcga gcgcgcccgc aacatcaccg aaacagcttt
     4561 ggttcgcggt ttgcagcgca ccaacggcgt agccaatcgc ttgaactact acaaccagtt
     4621 tttgggcacg gcggactatt ttgcgaaaga catcgctcgc tttgacgccg tgaaacccgc
     4681 cgatgtgcaa ctcgttgcac aaaccttctt taagccggac gcccgcagcg tggtctttgc
     4741 ggttcccggt aagaaagtga tcgaagacgt tcctaaaacg tccgaggaag aaaacaagaa
     4801 gcaagccgcc gacgctgggc cgagcaaagt tactatgccg gacgaagcgt ggcgcgccaa
     4861 tgcgccaaaa cccggcccgg cgccaaaatt caccctgccc atgcccgaac aggcaaaatt
     4921 gtccaacggc ctcaccatac ttctcgtgga gcgccataac ttgccattcg tttcggcgac
     4981 ggtttacacc atgagcggca gcgaactcaa tccgctcgac aagcccggcc tgtccagttt
     5041 tgtcgccggc atgctcatgg aaggcactac caatcgctcg ccattgaaat tcgcagagga
     5101 cgccgatcag atcggcgcga gcatcgattc ctcggccggg tacagcagca ccaccgtcag
     5161 cttgtcggcc ctgagttgga acgcaggcgc ggccctggac ttggcctcgg atgccatctt
     5221 gcatcccgca ttcgccgaaa aagaagtaga gcgcatccgc aaccagcgcc taactgctgt
     5281 cttgcaggaa aatgacgagc cgttcgcgct cgcgcaacgc accgcttcgc atctggtttt
     5341 tcccaacagt ccttacggat tttccgccat cgggaccgaa gcttcgaaca aagcgattat
     5401 gcgcaccgac ctgaccggat tctggcaaaa agcctacgtg ccgtccaatt ccgtgctggc
     5461 catttccggc gac
//
LOCUS       DASTPM010000428         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1370174, whole genome shotgun sequence.
ACCESSION   DASTPM010000428 DASTPM010000000
VERSION     DASTPM010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370174"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            296..796
                     /gene="smpB"
                     /locus_tag="VFR42_07510"
     CDS             296..796
                     /gene="smpB"
                     /locus_tag="VFR42_07510"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU0369038.1"
                     /translation="MAKAAKQQGRDVNKKGEQIVAQNRAASYNYHLLERMEAGLVLHG
                     TEVKALREGKANIRDAFVDFKPSGPWLVNAHIAQYMAGGPWNHEPLGNRKLLLHKNEI
                     AKWAGRTQSKGLTVIPLRIYFRDGIAKCEIALAQGKKQWDRRQDERTKEARKEAEAAM
                     YRNRRR"
     gene            804..2294
                     /locus_tag="VFR42_07515"
     CDS             804..2294
                     /locus_tag="VFR42_07515"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU0369039.1"
                     /translation="MQITQDTKSFGAVETDALVTYLFEDSEPISGRVAEIDVAAGGLL
                     KKLASSGELTGKTLEFTLIHAPAGLKAARLLLVGAGKKDSFSGATLRRLAGAALRYLK
                     ARSVKNFAFLARENGATADAAQALAEGFITANFETDKYKTEKKNGKEIDSITLVGFAD
                     AEKSAGEKSLLTGKIIAESQNFTRDLVNEPSNKLTPRVLADKAAAMAKEAGLAVDILD
                     EKKIAELKMGALLSVAQGSAEPPRMIVVTYIPANAKPGAPVIGLVGKAVTFDTGGISI
                     KPADGMEKMKYDMAGGATMLGAIRALAALKPNVKVICVVPSTENMPGGKAQKPGDIQT
                     ALSGKTIEVLNTDAEGRLILADAITYAKQLGVTHIVDAATLTGAIVVALANVNVGVFG
                     SNQEFTDKLLASAKATGEKMWQMPMDDDYREFIKGSFADIQNIGSGKGGGSITGAWFI
                     REFAGDTPWIHLDIAGTAWNDDTKPWLAKGPTGVALRTLVHLVQTF"
     gene            complement(2362..>2828)
                     /locus_tag="VFR42_07520"
     CDS             complement(2362..>2828)
                     /locus_tag="VFR42_07520"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HEU0369040.1"
                     /translation="GDALSFEGETGPYCQYAVVRIRGIRRKGAEQISEPTSAQEKLLR
                     DGSGALAELESIDAGKFLNAQESVAVWELLLQAGSLDHAADAAISAQEPAFVAKYAFQ
                     LAQAFNNFYHKHHILSEPDAEKRSFLLGLTEVVERQLIRALSLLGIQAPERM"
BASE COUNT          653 a          735 c          899 g          541 t
ORIGIN      
        1 gctggcgagc gccttctggt aatactgcaa agcggtgtcg tagtcctgga ggtcttccgc
       61 tttgcgagca gccttatagt gttcgccgcc ttgggggcac cctgcggcaa acaggcctgc
      121 tacggcacac aagatcagaa tggtcttacg ttgcatgcaa actccgtaat ggtagccagg
      181 cctcgacaaa aagctgtacc actctagcat tggcttagag gcgcgacaag aaccgcgggg
      241 ttggctgtcc cgcatctgtt aatggcagtt aaaaggaatg ctatattggg ttgttatggc
      301 caaggccgcg aagcagcaag ggcgcgatgt caacaagaag ggcgagcaga ttgtggctca
      361 gaaccgcgcg gcgagctata actaccacct gctcgagagg atggaagcgg gcttggtgct
      421 ccatggcacc gaagtgaagg ctttacgcga aggcaaagcc aacatcaggg acgccttcgt
      481 ggactttaag ccctccgggc cgtggctggt gaacgcgcac attgcccaat atatggccgg
      541 agggccctgg aaccacgaac ctcttggcaa ccgcaagctc ctgttgcaca aaaacgagat
      601 cgccaagtgg gctgggcgca cccaatcaaa aggtttaacc gtcataccct tgcggattta
      661 ctttcgcgat ggaatcgcca agtgtgaaat cgccctggcg cagggcaaga aacagtggga
      721 ccggcgccag gatgaacgca cgaaagaggc tcgcaaggaa gcggaagcag cgatgtatcg
      781 caatcggagg cgatagaacc gttatgcaaa ttacgcagga tacgaagtcg tttggagccg
      841 tggaaaccga cgcgctggtt acctatttgt ttgaggacag tgagccgata agcgggcgtg
      901 tcgcggagat tgacgtagcg gccggcggtc tgctgaaaaa gttggcgagc agcggtgaac
      961 tcaccggcaa gacgctagag ttcaccttga tccatgctcc ggcggggctg aaagcggcga
     1021 gattactctt ggtcggggca ggtaagaagg attcgtttag tggcgctacg ctgcgcagac
     1081 ttgccggggc ggccctacgc tatttgaaag cacgctccgt gaagaatttt gcatttttag
     1141 cccgtgaaaa tggagcgacc gcggacgccg cgcaagcgct ggccgaagga ttcatcactg
     1201 cgaactttga aaccgacaag tacaagaccg aaaagaaaaa cggaaaagag atcgattcga
     1261 tcacgctggt tggttttgct gatgccgaga aatcagcagg cgaaaagagt ttgttgacgg
     1321 gaaagattat tgcggaatcg cagaacttca cgcgcgatct ggtgaacgag ccgtcgaaca
     1381 agctgacacc gcgggttctg gcggataaag cggcggccat ggccaaggaa gccgggctgg
     1441 ccgtggacat tctggacgag aagaaaattg ccgagctgaa gatgggcgca ctgctgagcg
     1501 tcgcacaggg aagcgcggag ccaccgcgca tgattgtggt cacctacatc ccggcgaacg
     1561 cgaagcctgg tgcgccggtg attggcctgg tcggcaaagc agtgacgttc gacacgggcg
     1621 gcatttcgat caagccggcc gacggcatgg aaaaaatgaa gtacgacatg gcgggtggtg
     1681 caacgatgtt gggggcgatt cgcgctctgg ctgcgctgaa gcccaacgtg aaagtgattt
     1741 gcgtggtgcc ctccactgaa aacatgccgg gaggcaaggc acagaagccg ggcgatattc
     1801 agacagcgtt gtccggcaag acgattgaag tgctgaacac ggacgccgag gggcgattga
     1861 ttctggctga cgcaatcacc tacgcgaagc agcttggtgt cactcacatc gtggacgcgg
     1921 cgacgctgac cggtgcgatt gttgtggcgc tcgccaacgt gaacgtgggc gtgtttggtt
     1981 caaaccagga gtttacagac aagctattgg catcggccaa agcgacgggc gaaaaaatgt
     2041 ggcagatgcc catggacgac gactatcgcg agttcatcaa gggcagtttt gcggacatcc
     2101 aaaacatcgg aagcggcaag ggcggcggct cgatcacggg cgcctggttc atccgagaat
     2161 tcgcgggcga tacgccgtgg atccacctcg atatcgcggg caccgcgtgg aatgacgaca
     2221 ccaagccctg gctggccaag ggtccaaccg gcgttgctct ccgcacgctg gtgcacctgg
     2281 tgcagacctt ctaactaagc gtcttgaatt ttcttcgtag ggtcgcagca tgccgcgcct
     2341 cttatgtttt agtgcacgcg attacatgcg ctccggcgct tggatgccga gcagcgagag
     2401 cgcccggatg agttggcgtt cgacgacttc tgtgaggccc aggaggaagc ttcgcttttc
     2461 ggcgtcgggt tccgaaagga tgtgatgctt gtgatagaag ttgttgaaag cctgtgcgag
     2521 ctggaacgca tacttggcga cgaacgcggg ctcttgcgcg gagatcgctg cgtcggcagc
     2581 gtgatcgagc gatccagctt gaaggagcaa ctcccacacg gcgacgctct cctgcgcgtt
     2641 caggaatttt ccagcgtcaa tggattcgag ctcagcgagg gcgcctgaac catcgcgcaa
     2701 cagcttctcc tgcgcggacg tcggttcgga aatctgctcg gcacccttgc ggcggatgcc
     2761 gcgaatcctt accaccgcgt actggcaata aggtcccgtt tcgccttcaa aactgagggc
     2821 atccccga
//
LOCUS       DASTPM010000429         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_966475, whole genome shotgun sequence.
ACCESSION   DASTPM010000429 DASTPM010000000
VERSION     DASTPM010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966475"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..2035
                     /locus_tag="VFR42_07525"
     CDS             338..2035
                     /locus_tag="VFR42_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0369041.1"
                     /translation="MARVVLAVGCYAWTRLATTELAQQAWGVQDFMNAYLLYSFLILF
                     LLRLHGAADFAYRLTTLAVDLLFAGAITVFTGGPGIPYGVLWVFVVMTAAYRGSLRET
                     NLAATACALLLLVEGMVWRLWPQYFQPAGEADFSIDRLVSRGIFLVVASLVLGYLTVR
                     ERRLRAESALVARILGHAQVRMDQALGALFAEIAPLYLPLKTLVALRKGNVEEVFSWE
                     PEHPPSKLPVEAVRTVLAFSKLEAAAFSVPAHAWYFARNSQRPSRASNLLAFDAFGQT
                     VGGLSSEDLHSCFPASEFPSLMVSSFSFSEALKGRLILVGPSLHESSKEALRFLQRVV
                     EQIGPVVQNVYLSRDIRTQVEDQVRARLTRELHDGTIQSLLSADMQIEVLRRQGLCSP
                     GELEKRLAALQSLIHQQALNLRDLIEETKPLNFSPKELPDFLAELVARFRRETGISVR
                     LETQQDRITLEPGVCHEIVRIVQEGLSNVRKHSGARNVVVSLRAGEKGQSKLLIADDG
                     LGFGFRGLVTQTQLDAAHRGPSVIKQRVRLIGGELTIDSSPEYGARLEITIPDGFHG"
     gene            2028..>2313
                     /locus_tag="VFR42_07530"
     CDS             2028..>2313
                     /locus_tag="VFR42_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0369042.1"
                     /translation="MAEKSAKIRILVADDHPVFRYGLRTLLESEPKFTVVGEAADGSE
                     VAKLTSMLKPDFLMLDLAMPRLTGMEVLRELAFTHDQVRTIVLTAAIEKEQ"
BASE COUNT          481 a          608 c          707 g          517 t
ORIGIN      
        1 agaagtcaag aatcaacaca gcgactgcca cagaaagtcc tttgctattc gagaaagccc
       61 gcggttgtcc atacggttgg acttcaggcg aacaacatag gtttcccttt tttgcgccgg
      121 aggcatgggc ctaggcggca ggcgctgagc gacgaagcga tggcttgcgg gatagaggca
      181 agaatgctaa gtaggtatcc attaggtccc tccgagcagt gaggtgaagt gaggcgcaat
      241 gttgtggcgt ttccggatct cagagggaga ctttggccgc cggctgtgtg cttcttcagg
      301 agctcaagaa gtctttcgcg aggagaactg gctctgcgtg gcgcgcgtcg ttctggcggt
      361 gggctgttac gcttggactc gtctggccac gaccgagttg gcccagcagg cgtggggcgt
      421 ccaggatttc atgaacgcct accttctcta tagtttcctg attctgttcc tgctacgcct
      481 gcatggtgcg gccgacttcg cctaccgcct aacgacgctg gcggtcgatc tgctttttgc
      541 cggagcgatc acggtattca ccggaggccc gggaatcccc tacggggtcc tatgggtttt
      601 cgtcgtgatg acagccgcct accgcgggag tcttcgtgag accaatctcg ccgcgacagc
      661 gtgtgcgctg ctcttactcg tcgaaggcat ggtatggcga ctgtggccgc agtatttcca
      721 acccgcaggg gaagccgatt tcagtattga tcggctggtg tcgcgcggca tatttctggt
      781 tgtcgccagc ctggtgctcg gctatctgac ggtgcgagag aggcggctgc gcgccgagag
      841 tgccttggtg gcaagaatcc tcgggcatgc gcaagtcaga atggaccagg cattaggggc
      901 gctgtttgcg gagatcgcac cactgtattt gcctctaaaa acactcgtag cgctgcgaaa
      961 aggaaacgtt gaagaggttt tctcctggga gccggagcac cctccgagca aactcccagt
     1021 tgaagcagtc agaactgtgt tggcattttc aaagttggag gcggctgcct tttccgttcc
     1081 cgcgcatgcc tggtatttcg cgcgaaactc acagcggccc agccgcgcta gcaaccttct
     1141 ggcatttgac gcgtttgggc aaacggtcgg cgggttgtct tccgaggact tgcattcctg
     1201 tttcccagcc agcgagtttc cgtccctgat ggtgagctcc ttttcgttca gcgaggcatt
     1261 gaagggaaga ttgattttgg tcggtccctc gctccacgag agcagcaagg aagcactccg
     1321 ttttctgcag agggtggtag agcagatcgg cccggtagtg caaaacgttt atctctcaag
     1381 ggatattcgc acacaagtcg aagaccaggt gcgcgcccgg ctcacacgcg agctgcacga
     1441 tggaaccatc cagtcgctgc tgagtgcgga catgcagatt gaagttctca gacgccaagg
     1501 cttatgttct ccgggcgaat tggagaaacg gcttgccgct ctgcagtcgc tcatccatca
     1561 acaggccctg aaccttcgcg atctgatcga ggaaacaaag cccttgaact tcagccccaa
     1621 ggagctgccg gattttttgg cggaattggt agcaagattc cggcgtgaaa ccggtatctc
     1681 ggtccgtctg gagacgcagc aggacaggat cacgctggag ccgggcgtat gtcacgaaat
     1741 tgtgcgtatc gttcaggagg gactctcgaa cgtaaggaaa catagcggag cgcgcaatgt
     1801 ggtagtcagc ttgcgcgcag gcgagaaagg acaaagcaaa ctcttgattg ccgacgacgg
     1861 cttgggattt ggatttcgtg gtctggtgac gcagacgcaa ctcgacgcag cacatcgcgg
     1921 tcccagcgtg ataaaacaac gtgtgcgcct gatcggcggc gaactgacga ttgactcttc
     1981 tccagagtat ggagcacggc tggaaattac cattccggat gggttccatg gctgaaaagt
     2041 ccgcgaagat tcgtatcttg gtcgccgacg accacccggt tttccgttat gggctgcgga
     2101 cattgcttga gtctgaaccg aagtttacgg tcgttggcga ggcggcggac ggatcagaag
     2161 ttgccaaatt gacgagcatg ctgaaaccag attttctgat gttggatctc gctatgcctc
     2221 ggctcacagg aatggaagtg ctgcgggagc tggcgttcac ccacgaccaa gtacgcacca
     2281 tcgttctgac cgcggccatc gaaaaagaac aac
//
LOCUS       DASTPM010000430         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_101610, whole genome shotgun sequence.
ACCESSION   DASTPM010000430 DASTPM010000000
VERSION     DASTPM010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101610"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /gene="pstC"
                     /locus_tag="VFR42_07535"
     CDS             complement(<1..810)
                     /gene="pstC"
                     /locus_tag="VFR42_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU0369043.1"
                     /translation="MSTTSIHVPSADPVDDSRSFLSRLRNGDEIARLVTFLFAASVVL
                     ITVMLVYELWIASSLPRQKFGLAFFVTKLWDPIAEQFGALAFIYGTVVTAGVALLIAI
                     PLGIGAAIFLAELAPQKISDPLQFFIDLLAAVPSVIYGVLGVFIVIPMMRTWIAPTLK
                     ATLGFLPLFQGPAYGVGFLTAGIVLAIMVIPYIISVSREVLLSVPRDQREAALALGST
                     RWESTWRVVVPFARTGIMGSIFLALARALGETMAVTMVIGNTPSISSSLFAP"
     gene            complement(819..1841)
                     /gene="pstS"
                     /locus_tag="VFR42_07540"
     CDS             complement(819..1841)
                     /gene="pstS"
                     /locus_tag="VFR42_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU0369044.1"
                     /translation="MKRLSGVLVLALALAGVMLAQGGLINGAGATFPYPIYSKWFDQF
                     HKANGAQINYQSVGSGAGIKQVTEGTVDFGASDGPMNDDQLKAYRAKNGTGILHFPTV
                     LGAAVPTYNIPGVNETLNFTPDALAGIFLGRISKWNDPAIASANKGVNLPGNDIVVVH
                     RSDGSGTTYIWTDYLSKVSGDWKDKVGKGTSVNWPVGLGGKGNEGVSGLLKQTPNAIG
                     YVELIYAAQNKISYGTVKNLAGTFVKADLASVSEAASGASKTMPDDFRVSITNAPGKN
                     AYPISSFTWLLIPEKFKDATKRDTIKNFVKWAITDGQGDAEALSYARLPKEVVDKELK
                     ALSKVQ"
     gene            complement(2104..4125)
                     /locus_tag="VFR42_07545"
     CDS             complement(2104..4125)
                     /locus_tag="VFR42_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369045.1"
                     /translation="MKKLAAIVLSFFLMSGTAFADTPNDSDPPAQTSKKKSAKPAAKS
                     ATAVLAEQVEALRQSLEAQQQQIQQLKEELSRRDSQIGDVKTAAAAADAKAAEANTKV
                     AEVAASNADVKTTTTALSSDVADLKLGSDALKGAVQDTQKKIVAAESPSTIHFKGITL
                     TPGGFLAAETVTRTRAASADINTPLTGIPFPGNSLSKVSESNFTARQSRLSLLAEGKV
                     GSAKIGGYYEVDFLGTGVTSNNRQSNSYVWRTRQAFGQVSWENGWSLTGGQMWSLATE
                     TKKGITNRQEVPPMLVDSQYNVGFTWARQYGFRVVKSFHDKFSLAAAVEASQTTIGGR
                     GFSAVTNTSATGAVTTTQNFWTDAPGSGGGLYNFVDTTGYSTNKAPDFIVKAALDPGW
                     GHYELFGIVSEFRARIYPCGVVGTNATDTNPPATPTTFIGNCASATPTVPSSFGAFND
                     TSTGGGAGASARVPVGKKLDAGLKFVAGSGIGRYGSAQLADATARPDGTLALIRSAQW
                     LGSLEFHPSPKLDVYAYFGQEYGGRAGYQGYTTISVLKTPAIAATATSPAIPGTTTTT
                     IRLNQFGGYGSPFANNSGCSTENPPSTQLAPSAGGTCAGDIRVISEASFGFWHKVYAG
                     PKGGVRWGLQYSYLTKTGWSGNNGVPTAIGVSPKAVDNMLFTSFRYYIP"
     gene            complement(4405..>5256)
                     /locus_tag="VFR42_07550"
     CDS             complement(4405..>5256)
                     /locus_tag="VFR42_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0369046.1"
                     /translation="YNGLETRALLQVAQQTVEARQLTVDKVSALTQAKLKSDLDLSFA
                     NVDLARAKLLQLEARNNAEASLAVLSAMLGYPDQQNFDLAESSHEPEQPEPDVTPLIV
                     AATQHRPEVLALEDQLQAAEKLGRAEHDLWKPTVSALGAVGVAPVRNENLPNWYGAVG
                     VNVNIPVFNGFRYNARAKTADLQTEVAKQKLIDERNNLARDVRTSWQDSQRAYEKLAV
                     TKQLREQANLGLNLAQSRYNLGLGSIVELGQAELQKTEADIADTDAQYQYRLSRLVLA
                     YTIGEPK"
BASE COUNT         1082 a         1455 c         1539 g         1180 t
ORIGIN      
        1 gggagcgaaa agagagctgc taatagacgg agtgttgccg atgaccatgg ttaccgccat
       61 ggtttcgccg agggcgcgag cgagggcgag aaaaattgaa cccatgatgc cggttcgagc
      121 gaaaggaacc acaactctcc aggtcgattc ccagcgcgtg gagcccaatg ccaacgccgc
      181 ttcacgctgg tctcgcggaa ctgagagcaa aacttcgcgc gacaccgaga tgatgtaagg
      241 aataaccatg atggcgagaa cgatgccggc ggtaagaaat ccaactccgt atgccggtcc
      301 ttgaaacagc ggcaggaaac ccaacgtcgc cttcagcgtc ggagcgatcc acgtccgcat
      361 catgggaatt acgataaaca cgccgagcac tccgtaaatt acgctcggca ccgcagccag
      421 caaatcgata aaaaattgca gggggtcaga tattttttgc ggagcgagct cggcaaggaa
      481 gatggccgcc ccgattccga gagggatggc aatcagcaag gccacaccgg cggtaaccac
      541 cgtgccgtaa ataaaagcca gtgcgccaaa ctgttccgcg atcggatccc acagcttggt
      601 gacaaaaaat gccaatccaa atttctgcct gggcaacgac gacgcgatcc acagctcata
      661 gaccagcatt actgtgatca ggaccacgct cgccgcgaac aaaaaagtga ccagccgggc
      721 tatttcgtcg ccgttgcgca ggcgagaaag aaacgagcgc gaatcatcca ctggatccgc
      781 gctcggcacg tgtatggaag tagtagacac gcttgggctt actgtacctt gctgagagct
      841 ttgagttcct tgtcgaccac ttctttgggg agtctcgcgt aggacagcgc ttccgcgtcg
      901 ccttggccgt ccgtaatcgc ccatttcacg aagttcttga tggtgtcgcg ctttgtggcg
      961 tccttgaact tttcgggaat gagcagccag gtaaagctgg agatgggata ggcgttcttg
     1021 ccaggagcgt tagtaatgga aacgcgaaaa tcatctggca tcgtcttaga ggcgcccgat
     1081 gccgcttcgc tcacgcttgc caagtcggct ttgacgaagg tcccggccag attcttgact
     1141 gtgccatagc taattttgtt ctgcgccgcg tagatcagtt cgacgtagcc gatagcgttt
     1201 ggcgtttgtt tgagcaagcc actcacgcct tcgttgccct tgcctcccaa acccaccggc
     1261 cagttgaccg aggtgccttt gccgaccttg tctttccaat cgccgctgac cttggagaga
     1321 tagtcggtcc agatgtaggt ggtgccgctg ccgtcggagc ggtgaacgac gacgatgtca
     1381 ttgcccggga gattgacgcc tttgttagcg ctcgcgatgg ctgggtcatt ccatttggag
     1441 atgcggccga gaaaaatacc ggccagcgca tccggcgtaa aattcagcgt ttcattgacg
     1501 ccgggaatat tgtaggtcgg aacggctgct ccgagaacag tgggaaagtg taggatgcca
     1561 gtgccgtttt tcgcacggta ggctttgagt tgatcgtcgt tcatgggtcc gtccgaagcg
     1621 ccgaaatcca cggtgccttc agtaacctgt ttgatgcctg ctcccgagcc tacggactga
     1681 taattaatct gtgcgccgtt ggccttgtga aattgatcga accatttgga gtaaattggg
     1741 taaggaaaag tggcgcccgc gccgttaatc aatccgcctt gcgcaagcat tacgccggct
     1801 agggcaagcg ccagcaccaa cacacctgac aatcttttca tttttgtctc tccacaccaa
     1861 tcgcgccgtt gagcgggcga tatttaacca ggtcgggtcc acgccagtag gaagaatctt
     1921 ctgccagcga tgttacggga ttgttacacc ctgttcagag ttggttgaat ccggaatcag
     1981 ctcatttgct tggacttaga tggcgagaat tacaggtagt tgacggcgaa aagcaaaaaa
     2041 ggggcgccct ttcggtgtcg cccctttctc gttaccaggg attgaacgaa cgcaaaactc
     2101 caactacggg atgtaatagc ggaaggaggt gaacagcatg ttatcgacgg ccttgggtga
     2161 tacgccaatc gccgtcggta cgccgttatt tccagaccat ccggtcttgg tcaagtagga
     2221 atactgcagg ccccacctca caccaccctt gggaccggca tacaccttgt gccagaagcc
     2281 aaagctggct tcgctgatga cgcgtatgtc tccggcgcaa gtaccgcctg cactaggcgc
     2341 cagttgggtg gaaggaggat tttccgtgct gcaaccgctg ttattggcaa acggcgaacc
     2401 gtagccgcca aactggttga gcctgatggt cgtggtggtg gtgccgggga tcgcaggcga
     2461 ggtcgcagtt gccgcgattg ccggcgtttt taagacgctg atggtcgtgt agccctgata
     2521 acccgcgcgg cccccgtatt cctggccgaa ataggcgtaa acatcgagtt ttggcgaggg
     2581 atggaattca agcgagccca gccactgcgc actcctaatg agcgccaagg ttccatcggg
     2641 ccgggccgtg gcgtcagcca gttgagcaga gccgtagcgg ccgatgccac tgcctgcaac
     2701 aaacttcaag ccggcgtcga gtttctttcc gacaggcact cgggcgctcg cgcctgcccc
     2761 gccgcccgtg ctcgtatcgt tgaacgcgcc gaacgaactg ggcaccgtcg gcgtggccga
     2821 ggcgcagttg ccgatgaacg tagtcggggt tgcgggggga ttggtatcgg tggcgtttgt
     2881 gccaacgacg ccgcaaggat aaatgcgtgc ccggaattca ctgacgatgc cgaagagttc
     2941 atagtgaccc cagcccggat ccaaggcggc cttcacgatg aagtcggggg ccttgttagt
     3001 gctgtaaccg gtcgtgtcaa caaagttgta caacccgcct ccagacccag gcgcatcggt
     3061 ccagaaattt tgcgttgtgg ttactgcgcc ggtcgcgctg gtgttggtca cggcagagaa
     3121 accgcggcca ccaatggtgg tttgcgaagc ttctactgct gcggcaagag aaaacttatc
     3181 gtggaagctc ttgacaactc ggaagccata ctgccgcgcc caggtaaagc cgacgttata
     3241 ctgcgaatcg accagcatcg gcggaacttc ctggcgattc gttatacctt tcttggtctc
     3301 tgttgcaaga gaccacatct ggccacctgt gagcgaccag ccgttttccc aggaaacctg
     3361 cccgaaggcc tggcgcgtac gccaaacata gctgttgctc tgccggttgt tggaggtcac
     3421 gccggttccc aggaaatcca cttcgtagta gccgccgatc ttggcgctgc ccactttgcc
     3481 ttcggccagc aagctcaaac ggctctggcg cgcagtgaag ttgctttcgc tgactttact
     3541 gagcgagtta cctggaaagg gaataccggt caatggggtg ttgatgtcgg cgctggctgc
     3601 gcgggtccgg gtaaccgttt ctgccgccag gaatccgccg ggcgtgaggg taattccctt
     3661 gaagtggatc gtggaaggcg attccgcggc tacgattttc ttctgtgtat cctgcacggc
     3721 acccttcaag gcgtcgctgc cgagcttcaa gtcggcaacg tcggagctca aagcagtcgt
     3781 ggtggtcttc acgtccgcat tgctggcggc gacctccgcg accttggtgt tggcctcggc
     3841 ggccttggcg tcagcggcag ccgcagcggt tttgacgtcg ccaatctgcg agtcgcgtcg
     3901 cgacagctcc tcttttagct gttggatctg ctgctgttgt gcttccaggc tctggcggag
     3961 cgcttccact tgctcggcaa ggaccgccgt tgcagacttc gcagccggct tggcggactt
     4021 cttcttgctg gtttgcgctg gcgggtcact gtcgttcggt gtatccgcaa acgccgttcc
     4081 ggacatcagg aaaaacgaca atacgattgc tgcaagtttt ttcatttcct ctctcattga
     4141 ccgcgcgggc ggtcatcctt ccgtttctgc aatttcagtt cggaattacc tggtgagtac
     4201 gcgaacttac cccgcggtgg taacgtaggg ggcgcttctt acggacacgt caatcgaatg
     4261 tgaatatccg gtaacaggaa ttgttacaag ggatctcact gacacctcgg gagttacgag
     4321 ttgagagggt ttaaaaacca caccaagccg ccccgccggg ttccattgcc cgggtgctcc
     4381 ggtgttacac aattctcggt caaattactt cggctcgcct atggtatagg caagaaccag
     4441 gcgggacaaa cgatactgat attgggcatc cgtgtcggca atgtccgctt cggtcttctg
     4501 cagttcggcc tggccgagtt ccacaatgga gcctagaccc aggttgtagc gagattgcgc
     4561 aaggttcaag ccgagattgg cctgctcgcg aagttgcttg gtaactgcca gcttttcgta
     4621 agctcgctgt gaatcctgcc aggacgtgcg tacatcgcgc gcgaggttgt tccgttcatc
     4681 gatgagtttt tgtttagcca cttcggtctg tagatccgcg gtttttgcgc gggcgttgta
     4741 ccggaaacca ttgaaaaccg gaatgttcac attgacgccg accgcaccgt accaattggg
     4801 cagattttcg ttccgcacgg gcgcaacccc gaccgcgcca agagcgctga cggtgggttt
     4861 ccacagatca tgctctgcgc gaccgagctt ttccgcagcc tgcaattggt cctcgagcgc
     4921 cagaacctcg gggcggtgtt gcgtggccgc cacgatcaac ggcgtaacgt cgggttctgg
     4981 ctgctccggt tcgtgcgaag actcggccag gtcgaaattc tgctggtcgg gatagccgag
     5041 cattgcagaa aggaccgcca gcgaagcttc ggcattgttg cgagcttcca actggagcag
     5101 ctttgcgcgg gctaggtcta catttgcgaa gctcaagtct agatctgatt tcaatttggc
     5161 ctgggtcagt gcgctcactt tgtccaccgt caactggcgt gcctcgactg tttgctgcgc
     5221 gacttgcaac agcgcacgcg tctccaatcc gttgta
//
LOCUS       DASTPM010000431         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_159130, whole genome shotgun sequence.
ACCESSION   DASTPM010000431 DASTPM010000000
VERSION     DASTPM010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159130"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1144
                     /locus_tag="VFR42_07555"
     CDS             224..1144
                     /locus_tag="VFR42_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0369047.1"
                     /translation="MPNPVLQVENLTKRYGDVQAVRGVSFQVAEGEVFGLLGPNGAGK
                     TSTIEILEGLRDADGGVISVCGYDPRRQGDELKHEIGAALQSTALPEKLRVMEALRLF
                     AGFYKRRKNPDDLLKRFGLEEKRNAFYGQLSGGQKQRLALAIALINDPKVVFLDEPTA
                     GLDPQVRREIYDVIEELRRDKKTIVLTTHYIEEAERLCDRVAIVDHGQIIAQGTPREL
                     KQASADKTRIEVRLAREESEATLGTLEGVSDCRSLNGNYVLHCQRPPQAIVSLVKHLE
                     AQNNELVSLEIATPSLEDVFIELTGRRLRD"
     gene            1141..2091
                     /locus_tag="VFR42_07560"
     CDS             1141..2091
                     /locus_tag="VFR42_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369048.1"
                     /translation="MTNLLTLTRMRVRLALRNKMFFFFSIIMPIGFFFLYSGVFAKGE
                     PIVVAYLLGPVLGLNVMGSFWGLSATLVTFREQGILRRFHVTPATATDMLVSSILANY
                     ALTLPTVIAELLLAKFLFHVPSLGDLTSIFLLVSIGTISFGSLGLVIASVTNTMQETQ
                     VLNQLLWLPLIFLSGATIPFTSLSKTVQHIALFLPATYLVHGLKNAIINASGPAKILL
                     PLASLTVWFVLTLFLSSQLFRWEPEAKIGRRAKLYVAATAVPFLLLGVWEARYGRVIA
                     EEKVTMQRVEDSDRSEQRRKPPEREQSTDPSRLDDAKPQN"
     gene            2228..>2503
                     /locus_tag="VFR42_07565"
     CDS             2228..>2503
                     /locus_tag="VFR42_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU0369049.1"
                     /translation="MPVQKTEKIWHNGKFIKWDDANIHVLSHVVSYGSAVFEGIRCYD
                     TKQGPAIFRLREHMQRLINSAKIYRMELPFSVDEFGSVACELVRENKL"
BASE COUNT          533 a          669 c          751 g          550 t
ORIGIN      
        1 cagcggccag caggagcagc acaccaaggt ccgaccgaac gaagtccgcc aaccgtttgc
       61 tcacagcgcc tccaccaggg gctaaagtct accgaactct gggaagccgg ccagccgcgt
      121 aatttttcgg gccgacttgg cgtagtttca ctggcacggt tgccagacgg ctcgctcggc
      181 gggagcgcag atcctctgtt tcctgtaaac ttttctttct attatgccca acccggtatt
      241 gcaggtggaa aatctaacca agcggtacgg cgatgtgcaa gccgtgcgcg gcgtcagctt
      301 tcaagtggcg gaaggcgaag tatttgggct gctgggcccg aatggcgccg gaaaaaccag
      361 caccattgaa attctggagg gcttgcgcga cgcggacggc ggagtaattt ccgtctgcgg
      421 ctacgatccc cgcaggcaag gcgacgaact gaagcacgag attggcgccg cgctgcaatc
      481 cacggcactg ccggaaaaat tgcgggtgat ggaagcgctg cggctgttcg cgggatttta
      541 caaacgacgg aagaatccag acgatttgct gaaacgcttt ggtctggaag aaaaacgtaa
      601 cgcgttctac ggccagcttt ccggtgggca gaagcagcgg ctggcgctgg cgattgcgct
      661 gattaatgat ccgaaagtgg tttttctgga cgaaccgaca gcagggctcg atccgcaggt
      721 gcggcgggaa atttacgacg tcatcgaaga gctgcggcgc gacaaaaaga ccatcgtgct
      781 gaccacgcac tacattgaag aagcagagcg gctctgcgac cgcgtggcca tcgtggatca
      841 cgggcagatc attgcgcagg gcacgccgcg cgaattgaag caggcttcgg cggacaaaac
      901 gcgtatcgag gtgcggctgg cgcgggaaga atcggaagcg accctgggaa cgttggaagg
      961 agtgagcgac tgccgctcgc tgaacggcaa ctacgtgctg cattgccaac ggccgccgca
     1021 ggccatcgtt tcgctggtga agcatctgga agcgcagaac aatgaactgg tgagcctgga
     1081 aatagccacg ccgtcgctgg aagacgtatt catcgaattg accggaagga ggcttcgcga
     1141 ttgacgaacc tgcttacctt gacgcgtatg cgcgtgcgcc tcgcgctgcg caacaaaatg
     1201 tttttctttt tcagcatcat catgccaatc gggtttttct ttctgtattc cggcgtgttt
     1261 gcaaagggcg agcccattgt tgtcgcgtat ctacttggcc cggtgctcgg cttgaatgtg
     1321 atgggaagtt tttggggatt aagcgcgacg ctggtgacgt tccgcgagca aggcatcctg
     1381 cggcgatttc acgttacgcc ggcaacggcg acggacatgc tcgtgtccag cattctggcc
     1441 aattatgcgc tgaccctgcc gacggttatc gcggaactcc tactggctaa atttctgttc
     1501 catgttccca gcctgggcga cctgacttcg atcttcctgc tggtttcgat tggaacgatt
     1561 tccttcggtt cgcttgggct ggtgatcgcc agcgtgacca acaccatgca ggaaacgcag
     1621 gtgctcaacc agttgctgtg gctgccattg atttttcttt ccggtgcgac gattcctttc
     1681 acgtctctgt caaagacggt tcagcatatt gcgttgtttc ttcccgcgac ctatctggta
     1741 cacggactga aaaatgccat catcaacgcc tctggaccgg ctaagatttt gctgccgctg
     1801 gcttcactga cggtttggtt cgtattgact ctctttcttt cttcgcagct ctttcgctgg
     1861 gagccggagg ccaagatcgg ccggcgcgcc aagctatacg ttgccgctac ggcggttcct
     1921 ttccttctac tgggtgtttg ggaggcgcgt tacggccgag tgatcgcgga ggagaaagtg
     1981 acgatgcagc gagtggaaga ttcggacaga tccgagcaac ggcgcaagcc cccggagcgg
     2041 gaacaaagca cagatccctc caggctcgac gacgcgaagc cgcagaacta gtggtttctg
     2101 agggctgagt ttgcagtgcg ccaaattggt tctggcgtag cagtgttttc acggcacatt
     2161 aaattgacgc tggttggtgc gtaccgtatt ctgcgaacag agcggatccg agcctaaggg
     2221 agcttttatg ccggttcaga agacagaaaa aatctggcac aacggaaaat tcatcaagtg
     2281 ggacgacgcg aacatccacg ttctatcgca cgtggtgagc tacggctccg cggtatttga
     2341 aggcattcgc tgctacgaca ccaagcaggg accggcgatt ttccgtttgc gcgaacacat
     2401 gcagcggctg atcaactccg ccaagattta tcgcatggag cttcccttct ccgtggacga
     2461 attcggcagc gtcgcctgcg agctagtgcg agagaacaag ttg
//
LOCUS       DASTPM010000432         4310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_937858, whole genome shotgun sequence.
ACCESSION   DASTPM010000432 DASTPM010000000
VERSION     DASTPM010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937858"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFR42_07570"
     CDS             complement(<1..459)
                     /locus_tag="VFR42_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369050.1"
                     /translation="MASPGARKTLLFATASLLAFLVLLFAVAYGKKRSAELRTRDWIV
                     QVLEQKFQSNVELADFHVRIFPRMRVSGEGLSLHYWATPDAPPLIRVETFSFELGLLG
                     IFRAPHRIQSVRLKRMVLTIPPKGSRQPAAYRPVSRAKVSQVIVGSIEVED"
     gene            complement(493..1026)
                     /locus_tag="VFR42_07575"
     CDS             complement(493..1026)
                     /locus_tag="VFR42_07575"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEU0369051.1"
                     /translation="MTRKIVCLLEAALLMVCGAAAQIPSARDVVKPAPFASFDPVARG
                     KQLDVAVVMKIKDGFHVNARETTFDYLIPTDLKAEPAEGFKIGAVKYPQGTLHKFSFA
                     KDQALNVYTDTVVLFVPVTLGASAPLGEQHLALKLRYQACNNEVCLPPVTLPVDAVIH
                     VSSGESRPSHPELFGKP"
     gene            complement(1023..1361)
                     /gene="cyaY"
                     /locus_tag="VFR42_07580"
     CDS             complement(1023..1361)
                     /gene="cyaY"
                     /locus_tag="VFR42_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEU0369052.1"
                     /translation="MPMLEERDFQKKCEAALDELKRRLLDAGDEHGFDVEGESGKLEV
                     VFEEPTPAKFVVSPNTPVREIWISALSTSFKLGWSEARNAFVLEKTGEDLFAVLSRVV
                     SQQLGEEVEV"
     gene            complement(1365..1877)
                     /locus_tag="VFR42_07585"
     CDS             complement(1365..1877)
                     /locus_tag="VFR42_07585"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0369053.1"
                     /translation="MNKGTLLIVAATLLMTGSGCKKSHSASTFQSVQMKSLEDSSVTV
                     GDYHGKVVLVNFWATWCDPCRVEIPWLIEMQDKYGAQGFTVLGIAMDEEGKSAVAPFV
                     AKERYEVNGQRLPMNYPIVVGNEKVAEKFGGLLGYPTSILISRDGKVLKRTTGLIDYQ
                     ATTKMIEGAL"
     gene            complement(1926..2825)
                     /locus_tag="VFR42_07590"
     CDS             complement(1926..2825)
                     /locus_tag="VFR42_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU0369054.1"
                     /translation="MTPDVSLVAAFIAGLVSFLSPCVLPLVPGYISMLSGIGMEQLRQ
                     GQQPRGSLFASALSFVVGFSAVFIAFGASASAVGSFLQENRYTLAPIAGALIILFGLH
                     LIGALAKLKPIPGMVIGAIFVVLGIVALIRGDELFAGLGAVHFFSLSIIGFLGPALAR
                     WLNRDVHLRNTSKQPGILSGFFLGFAFAFGWTPCIGPILTTVLALAANSATIGRGVLL
                     LAFYSAGLAIPFLLAAIGIVQFLKFYQKFRGYLHAVELGSGILLLFVGGLVFTNKLTW
                     LVRKLSFLNSVALWLERALTHGK"
     gene            3019..3762
                     /locus_tag="VFR42_07595"
     CDS             3019..3762
                     /locus_tag="VFR42_07595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0369055.1"
                     /translation="MNQQLNKPSIGAGVAFAYFRRLSDDMGWTPNKVTVLRVLVGFVA
                     VGLFGHGTWANLAGVVLTVASIALDALDGHIARKKRMATSLGAQIDILGDRMIENMFF
                     TYFAVVGMVSLWLPVFFFARGAATDFLRGLALKAGYSGWGANAMLQTWWGRAFVASRW
                     SRGLYAALKCACFCYLGLELALTRGPVALLGTLPGEAHLTIRAIAQLLTWATAAFCLL
                     RGLPVLIEGWRFFAREAVTVRPRKSAEVA"
     gene            3759..>4310
                     /locus_tag="VFR42_07600"
     CDS             3759..>4310
                     /locus_tag="VFR42_07600"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU0369056.1"
                     /translation="MSIAFFDIDGTLLPHPSLERRFFRKLFGAGKIPVANCLRWAAEM
                     FRHRGAEFSVVAQSNKTYLRGVSTGVISEMSRETRWVPEFFPAALQRVWWHALRGDTV
                     VLVTGTLAPLAEIVQSALERELLLRGVESRIPAIATQLAIQDGCWTGRVSGSPMLGEA
                     KAAAIRGFVQARALPLQESSAYGD"
BASE COUNT          899 a         1264 c         1223 g          924 t
ORIGIN      
        1 gtcttcaact tctatggatc ctacaatcac ctgcgaaact ttcgctctgc ttaccgggcg
       61 gtacgcagcc ggttgacgcg agcccttcgg cgggatggtg agcaccattc gtttcagacg
      121 aacgctttga atgcgatgcg gcgcgcgaaa aatgcccagc aagcccagtt cgaacgagaa
      181 ggtttcgacg cggatgagcg gcggcgcgtc tggggtcgcc caatagtgga gcgagagccc
      241 ttcgccggaa accctcatgc gcgggaagat cctcacgtga aaatccgcca gctcgacgtt
      301 gctctgaaac ttctgctcca gcacttgcac gatccaatcg cgcgtgcgca gttcggcact
      361 gcgttttttt ccatacgcta ccgcgaaaag cagaaccagg aaggccaaga gcgacgcggt
      421 ggcaaaaagc agagtttttc gagcacctgg ggaggccacg aaaggaagtg tcgcaaacag
      481 gtggtggttt cgctacggtt ttccgaacaa ttccgggtgg ctagggcgag attctcccga
      541 acttacatgg attactgcat ccacgggaag agttaccggg ggcaagcaga cttcgttgtt
      601 gcaggcctgg tagcgcaact taagagcaag atgctgttcg ccgagtggcg cgctcgcgcc
      661 cagcgtgacc ggaacaaaca aaaccacggt gtcggtgtag acgttgagcg cctgatcctt
      721 ggcaaaactg aatttatgca gcgtcccctg ggggtacttc accgcaccaa tcttgaagcc
      781 ttcggccggt tcagccttca ggtcggtcgg gatcaggtaa tcgaaagtag tttcacgcgc
      841 gttgacgtga aagccgtcct tgatcttcat caccaccgca acatccaact gcttgccgcg
      901 ggccaccggg tcaaacgatg cgaagggtgc cggcttgacc acatcgcgcg ccgaggggat
      961 ctgtgcggcc gctccacaga ccatcaacag ggccgcctca agcagacata caattttgcg
     1021 cgtcatactt cgacttcttc tcccagctgc tgggaaacca cgcggctcaa tacggcaaaa
     1081 agatcttcgc cggtcttttc cagaacgaaa gcgtttctcg cttcagacca tcccagctta
     1141 aaactggtcg aaagcgcgga gatccagatt tcgcgtaccg gcgtgttggg cgaaaccaca
     1201 aactttgccg gcgtcggctc ttcaaagacc acttccagct tcccgctctc gccttctacg
     1261 tcaaagccat gttcatcgcc ggcgtccagc agccgccgct tcaattcgtc cagcgccgcc
     1321 tcgcattttt tctgaaaatc gcgctcctcc agcatcggca ttgcttacag cgctccctcg
     1381 atcatctttg tcgtcgcctg gtagtcgatc aatccggtag tgcgcttcaa cacctttccg
     1441 tcgcgagaaa tcagaatgct ggtcgggtag cccagcaagc cgccaaattt ttcggctacc
     1501 ttttcattgc cgaccacgat cggatagttc attggcagcc tttgtccgtt cacttcatac
     1561 cgttccttgg ccacaaacgg ggcaaccgcg gatttaccct cttcgtccat ggcgatgccc
     1621 aggacggtga atccctgagc gccatatttg tcctgcattt cgatcagcca gggaatttca
     1681 acgcggcacg gatcgcacca agtcgcccag aaattcacca gcaccacttt tccatggtag
     1741 tcgcccaccg tcacactgct atcttccagc gacttcattt gaacgctttg aaaagtgctg
     1801 gcgctgtggg attttttgca gccgctgccg gtcattagaa gtgttgcagc gacgatcagt
     1861 aaggttccct tgttcatgac gctcctaaag aaattatccc tcattgcggg gatttcgtcg
     1921 cggcgctact ttccgtgggt caaggcgcgt tccagccata acgcgacact gttcaagaaa
     1981 ctcagctttc gcaccagcca ggtcagtttg ttggtaaaga ccaggccgcc cacaaagagc
     2041 aacaggattc cgctgcccag ttcgaccgca tgaagataac cgcgaaattt ctggtagaac
     2101 ttgaggaact gtacgatccc gatcgctgcg aggaggaaag ggatcgcaag tcccgcggag
     2161 taaaaggcca gcagcaacac gccgcgcccg atcgtcgcgc tgtttgcggc aagggccaac
     2221 accgtcgtca ggatgggccc gatgcacggc gtccagccga acgcaaaggc gaatcccagg
     2281 aaaaatccac tcaggatgcc aggctgcttg ctggtgttgc gcaggtgcac atcgcggttc
     2341 agccaacgag ccaacgcagg cccgagaaaa ccgatgatcg agagcgagaa aaagtgtacg
     2401 gctccgagtc cggcgaacag ttcgtcgccg cggatcaacg ccacgattcc cagcacgaca
     2461 aaaatcgctc cgatgaccat tcccgggatc ggcttgagct tcgccaatgc gccaatcaaa
     2521 tggagtccaa acaaaatgat cagcgctccg gcaattggcg ccagcgtgta gcggttttcc
     2581 tgaaggaagg agcccacggc gctggccgaa gcgccaaacg caataaatac cgccgagaat
     2641 ccgactacga acgagagagc ggaagcaaac aaggagccgc gcggctgctg cccttggcgt
     2701 agctgctcca tgccgatgcc gctgagcatg gaaatgtagc caggtaccag tggcagcacg
     2761 cagggcgaaa gaaacgacac caatcccgca atgaaagcgg cgacaagcga gacgtccggc
     2821 gtcatgcgtc aggaatctcc gaaaagactt cgatgaacga gcgcgcgcaa aggtgtcgcg
     2881 gatcgatgta aaagacgtgt catgccggca aagtgtattc tcacacagaa tctattttcg
     2941 aggcgtgtgg ctgacacctg gcggctgcag ttggcggcgg ctggctcgac tgctttctgt
     3001 tccgcaatgt aaagctattt gaatcagcaa cttaataagc cgtcgattgg cgctggcgtt
     3061 gcttttgcct acttccggag gctttccgac gacatgggtt ggacgcccaa caaagtgacc
     3121 gtgcttcgcg tactggtggg cttcgtagcg gtcggccttt ttgggcatgg cacctgggcc
     3181 aacctggccg gcgtcgttct tacggttgcc tcgatcgcgc tggacgccct ggacggccat
     3241 atcgcgcgaa agaaacggat ggccacatca cttggcgctc agatcgacat cctcggtgac
     3301 cgcatgatcg aaaatatgtt cttcacctat ttcgccgtag tgggcatggt ttcgttgtgg
     3361 ctgccggttt tcttttttgc tcgcggtgcg gcgacggact ttctgcgcgg ccttgcgctc
     3421 aaagccgggt actccggttg gggcgccaat gccatgctgc aaacctggtg gggccgggcg
     3481 tttgtcgcct cacgctggag ccgcggactc tatgccgctc tcaagtgtgc gtgcttctgc
     3541 tatttgggcc tggagttggc gttgactcga gggccggtgg cgcttctggg tacgctcccc
     3601 ggcgaagcgc atctcaccat tcgcgccatc gcgcagcttc ttacttgggc caccgcggca
     3661 ttctgtttgt tgcgcggact gccggtgctg atcgaaggtt ggcgcttctt cgcgcgcgaa
     3721 gcagtaacgg tccggccgcg taaatccgcg gaggtagcat gagcattgcg ttttttgaca
     3781 tcgacggcac gctgctcccg catccttcgc tggagcggcg ttttttccgg aagctgttcg
     3841 gcgccggaaa aatccctgtc gccaattgtc ttcgctgggc cgctgaaatg tttcgccacc
     3901 gaggagcaga gttttctgtt gtcgcacaat ccaacaagac ttatctgcga ggagtctcca
     3961 ctggcgtcat ctccgaaatg tcgcgcgaaa cccgctgggt gcccgaattt tttccagccg
     4021 cccttcaacg tgtttggtgg cacgcattac gcggcgacac tgtcgtcttg gttacgggaa
     4081 ccttggctcc tctcgcagaa attgtgcagt cggcgctgga gcgtgagctg ctcctgcgcg
     4141 gtgtggagag tcgaattccc gccattgcta ctcagctcgc aatccaggat ggctgctgga
     4201 ccggccgcgt ttccggttca ccgatgttgg gcgaggccaa agccgctgcc atcaggggat
     4261 tcgtgcaggc gcgcgcgcta cccctgcaag aaagttcggc ctatggggac
//
LOCUS       DASTPM010000433         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_534384, whole genome shotgun sequence.
ACCESSION   DASTPM010000433 DASTPM010000000
VERSION     DASTPM010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534384"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFR42_07605"
     CDS             <1..354
                     /locus_tag="VFR42_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369057.1"
                     /translation="RRARKVSAWTTFDLLLNYTFNLPPPAPAEVPGFAKDGGKNVRSG
                     KDGKEKNVVPVSTAEYGCNNWKWWLNNTTVTLGLQNVGDADPPFVAGSFENGYDESLA
                     TIKGRFWYVGLKKRF"
     gene            936..>2372
                     /locus_tag="VFR42_07610"
     CDS             936..>2372
                     /locus_tag="VFR42_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369058.1"
                     /translation="MLVAFIWGVWHGMMQTYGFCRIYDAKVGSFAELTRRLDFAVCGI
                     WFATAVLLSPQRMTDTLETYYSAGGPFIPPAVLRAGQQGLLALALLISALFLANFIWL
                     WISGKRPSPVKLVLLLTSISFWWYCNNIVASVLVGVALFEVFHDVQYLSLVWIYNRKR
                     VESDSSIGGFMRFVFRRSGALVGVYIGLVLAYGGLSLSKSYVGIDAVKNILTGVVTAS
                     ALLHFYYDGFIWKVREKSTRQSLGIAGGTADVSLGGFLPGWALHGAKWVAVFVIPLGL
                     LWFAEIRSDRGKLERLGSIVADLPTSARAHVNYGTELQEAGRVDEAAKEFGEAIRLNP
                     GSAKAHVNLGSLLTAKGEFDAADSHFEKALRIEPSNAEYHSGYAYLLDQLDRKDQAAA
                     ECETAIRLAPKSPQAHYGYGAFLEKHGQPQEAIAKYRQALQYDPNHVDAHIDLGNLLF
                     ETGETAEAREHFQKASILNPKLAQPHNYL"
BASE COUNT          484 a          587 c          709 g          592 t
ORIGIN      
        1 agacgggcgc ggaaggtgtc tgcgtggacc acgttcgacc tgctcctcaa ctacacgttt
       61 aatctgcctc cgcctgcgcc agcagaagtg cctggcttcg ccaaggacgg cggcaaaaac
      121 gtgagaagcg ggaaggacgg caaggagaag aatgttgtcc ctgtatcgac ggccgagtat
      181 ggctgtaaca actggaagtg gtggcttaac aacaccacgg ttacgttggg cctgcagaac
      241 gtgggcgatg cagatccgcc gttcgtggca ggcagcttcg agaacgggta cgatgaatca
      301 cttgccacca tcaaaggccg gttctggtac gtcggactaa agaagcgatt ctaaagctat
      361 aacattcgtt aattcacagc ggccgggctc gacaatgggc ccggccgttt tgctttgcgc
      421 attttgtgtc ctgtcgggca cagtcgagaa atcgcgccgg aattattttc taagcggcgg
      481 ctgcgcggat gtgattttga gttttgtgcc tctcaaatca tctcaggatt gattttcgca
      541 gcgctttgag agaaacttgg ctccgctaag gagaaggcat gcagttcgtc ccggggattt
      601 catctgcgca aacgataccc gctgcgccgg cgccgacacc ggctgggcgc aagcctaact
      661 tgtggattct ggacagttgg cgggacttgg tactttacgt ctgcacaccg ttgttgctgg
      721 tgccgatctt cattggggca cagggtctct ggagcgcgga ggacatttac ctcttcgttg
      781 ccgcctttgg cgcaatggga catcacttgc cgggaatgat tcgagcgtac ggcgaccgtg
      841 cgctctttca gcgtttccga tggcgtttca tttgtgcgcc gatttttttg gtcgttgtct
      901 gtgcggcttt ttccgtgtgg gacctgaagg gaattgtgct ggtggcgttt atctggggcg
      961 tctggcacgg aatgatgcaa acgtacggct tctgccggat ttacgatgca aaagtcggct
     1021 cctttgctga gctaacgcgt cgcctcgatt tcgcggtgtg cgggatttgg tttgcaacag
     1081 ctgttcttct ttcgccgcaa cggatgacgg acacgctgga aacgtactat tccgcaggcg
     1141 gaccttttat tccgccggcc gttcttcgag ctggacaaca aggtttgctt gcgctggcac
     1201 ttctaatttc agcgctgttt ctagcgaatt ttatttggct gtggatcagc ggaaagcggc
     1261 cgagtccggt gaaactggtg ctgctactta caagcatttc gttttggtgg tattgcaaca
     1321 acatcgtagc cagcgtgctc gtgggcgtcg ctttattcga agtgtttcac gatgtgcaat
     1381 acctctcgct ggtctggatt tataatcgga aacgggtcga gagtgacagc tcgatcggtg
     1441 gtttcatgcg ctttgtgttc cggcggagtg gcgcgctggt gggagtgtac ataggcttgg
     1501 tgctcgccta cggtgggctg agtttgtcca aatcgtatgt cggcatcgac gccgttaaaa
     1561 atattcttac cggcgtcgtg actgcatcgg cactgctcca tttctactac gacggattca
     1621 tttggaaagt tcgcgagaag tcgacgcggc aatcgcttgg tattgccggt ggaacggcgg
     1681 atgtctcgtt aggtggtttt ttgcccggct gggctttgca cggcgcaaaa tgggtcgcgg
     1741 tttttgtcat tcctctcgga ttgctctggt ttgccgaaat acgaagcgac cggggcaaac
     1801 tcgaacgttt gggctcgatc gttgcggatc ttccgacgag cgcgcgggcg catgtcaatt
     1861 acggtactga actgcaggag gccgggcgag tcgatgaagc agctaaggaa ttcggtgagg
     1921 cgatacgttt aaatcccggc tcggcaaagg cgcacgttaa cctcggatcg ctattgacag
     1981 cgaagggaga gttcgacgcg gcggacagtc actttgagaa ggcattgcga atcgagccga
     2041 gcaacgctga atatcattct ggctacgcgt acctcttgga ccaactcgat cgaaaggatc
     2101 aagccgcagc tgaatgtgag acggctattc gtctggcccc gaagtctccg caagcgcact
     2161 acggctatgg cgcgttcctg gaaaaacatg ggcagccaca agaagcaatc gcgaaatatc
     2221 gccaggcgtt gcagtacgat ccaaatcacg tcgacgctca tatcgatctt ggcaatttgc
     2281 tctttgaaac tggcgagact gccgaagcga gagagcattt ccagaaggca tccatcttga
     2341 atccgaaact tgcgcagccg cataattatc ta
//
LOCUS       DASTPM010000434         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_217556, whole genome shotgun sequence.
ACCESSION   DASTPM010000434 DASTPM010000000
VERSION     DASTPM010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217556"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VFR42_07615"
     CDS             <1..332
                     /locus_tag="VFR42_07615"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0369059.1"
                     /translation="FPLETWNTRAGHFLKSTIEFRVEDFVMHWIWEGLIGLLAGAVAK
                     FIMPGKDPGGIIITMLLGIAGSVVATYLGSLIGWYHEGDKAGFLMSVAGAVLLLAIYH
                     LFRKKSA"
     gene            454..1242
                     /locus_tag="VFR42_07620"
     CDS             454..1242
                     /locus_tag="VFR42_07620"
                     /inference="COORDINATES: protein motif:HMM:NF004818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0369060.1"
                     /translation="MASESQKDLLGKVALITGGTSGIGRDTAVLFARHGANVVLTGRR
                     EAEGNETVKLAQAAGGDAIFLQGDVSKSADVQRFVQKTVEKFGRLDVAFNNAGIEGKW
                     MPIVDLPEAQFDEVTNINVKGVWLCLKYEMQQMLKQGGGGAIVNMSSVAGLMGNAGSS
                     IYAASKHAVIGLTRTAALEGASQGIRVNTVCPAVIETPMADRAFADPEAIKRVRAMHP
                     LGRFGKPMEIAEAVLWMCSDKSSFMTGHEIVLDGGMLAGPNPAA"
     gene            1419..1703
                     /locus_tag="VFR42_07625"
     CDS             1419..1703
                     /locus_tag="VFR42_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369061.1"
                     /translation="MTRKNLGAMLAVAAVLSLPLAGCKDAKTLQENEQLKAKVAELQK
                     ENGQVGNDLETMTTARDALMQENEKLKAQIKALKSKGSAKKKSSRKRHRS"
     gene            1783..2028
                     /locus_tag="VFR42_07630"
     CDS             1783..2028
                     /locus_tag="VFR42_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369062.1"
                     /translation="MKSFEVQFRFQDRSEGAVEKTLNVDASNLPGAVGKAAREFVKSL
                     DRKQRFDMNKNGLEITAKPVAGAAEAQAEPSSKAATN"
     gene            2185..2541
                     /locus_tag="VFR42_07635"
     CDS             2185..2541
                     /locus_tag="VFR42_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0369063.1"
                     /translation="MKFLCLVYGEEKEIAAMTDDECMDYDRSLRDAGRCLASEALQPV
                     RTAASVRLRNGKVSVRDGPFTETKEILAGFYLIDAADLNDAIQVASKMPPARVGTIEV
                     RPVRELQSTSGERRRV"
     gene            3231..3602
                     /locus_tag="VFR42_07640"
     CDS             3231..3602
                     /locus_tag="VFR42_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0369064.1"
                     /translation="MPLEISIRESADVTILDLRGRATISDGESDLLRASLDQLIANGA
                     RKLVLNLAELTHVDSSGFSVIVKVCVSARDKGGDLRVVRPCGPALVAFNVLRLLDLIR
                     AFDNEADALASFRRSGSSAQP"
     gene            3628..>3945
                     /locus_tag="VFR42_07645"
     CDS             3628..>3945
                     /locus_tag="VFR42_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369065.1"
                     /translation="MRALALIHLDHCSPQQLSIHRKQTPLGPGSFCFAAGRTTGNKLI
                     KPTPRAALDLAARRPTFYLTQVTLYFLELIAHAHGATMPDSLDDFQRTRADLNEEILG
                     CGHL"
BASE COUNT          832 a         1242 c         1073 g          798 t
ORIGIN      
        1 gtttcccatt agaaacatgg aacacccgcg ccggtcattt cctgaaatct acaatcgaat
       61 ttcgcgtgga ggatttcgtt atgcactgga tttgggaagg gcttattggc ctgctggccg
      121 gagcggttgc gaagttcatc atgccgggca aggaccccgg gggcattatc atcaccatgc
      181 tcttgggcat cgccggctcc gtcgtagcca cgtatcttgg ttccctcatc ggctggtatc
      241 acgagggcga caaagcagga ttcctcatgt ccgtggcggg cgcggtgctc ctgttggcta
      301 tttatcatct gtttcgcaag aagtcggctt gaagactgaa gcagcgcggg tctgtttgta
      361 gcgacggcgt ttgctagaat ctagctctca ttggcagcgg tcaactcaga agcacgcccg
      421 cgaaaatcat taaatgcaaa atcggagaac cacatggcaa gcgaatcgca aaaagacctg
      481 ctcggcaaag tagctctcat caccggcggc acttccggca tcggacgcga caccgccgtc
      541 ctgtttgccc gtcatggcgc caacgtcgtt ctcaccggcc gccgcgaagc cgaaggcaac
      601 gaaaccgtaa agctggcaca agccgcgggt ggcgacgcca tcttcctgca aggcgacgtc
      661 tccaaatccg ctgacgtgca aaggttcgtc cagaagaccg tggaaaaatt tggccgcctg
      721 gatgtggctt tcaacaacgc cggcatcgag ggcaagtgga tgccgatcgt cgatttgccg
      781 gaagcgcagt ttgacgaggt gaccaacatc aacgtgaaag gcgtttggct ctgcctgaaa
      841 tacgaaatgc agcagatgct gaagcaaggc ggaggaggtg ccatcgtcaa catgtcctcc
      901 gtcgcgggtc tcatgggcaa cgccggctcc tcgatctacg ccgccagcaa acacgcggtg
      961 attggcctga cacgtaccgc tgcgttagaa ggcgccagtc agggcattcg cgtcaacacc
     1021 gtgtgccccg cggtgattga gacgcccatg gccgatcgcg ccttcgccga tccagaagcc
     1081 ataaagcgag tccgggccat gcatccgctc ggccgctttg gcaaaccgat ggagatcgcc
     1141 gaagccgtct tgtggatgtg ctccgataaa tcctccttca tgaccggcca cgaaatcgtg
     1201 ctcgacggcg gcatgctcgc cggccccaat cccgcggcct gagcgtaata cgcggatgat
     1261 gcgaggtcct gtcccgcttg cggggctggc gacctcctca aacttgcgcg atctacgaaa
     1321 ctcctttacg ctgactcagt caccagcggc gtcttccctc ctcgcgcgct acaggttgct
     1381 tcattccaaa tccaattctg gtcgacgaag gatgacgcat gacgcgaaaa aaccttgggg
     1441 cgatgcttgc ggtggccgcc gtgctcagtt tgcccttggc tggctgcaag gacgcgaaaa
     1501 ccttgcaaga gaacgagcaa ttgaaggcca aggtagccga actccagaaa gagaacggcc
     1561 aagtgggcaa cgatctcgaa accatgacca ccgcccgcga cgcactaatg caagagaatg
     1621 aaaagctcaa ggcccagatt aaggccctca agtccaaggg ctccgcgaag aagaaatcca
     1681 gccgcaagcg gcatcggagc taaggtcacg gcaatcttga attcccaaat agtttgaacc
     1741 cggctctgat tctgtttacc gttagaaaag gagagtacgc gtatgaaatc gtttgaagtg
     1801 caatttcgct ttcaggatcg cagtgaagga gcagtggaaa agacgctcaa cgtcgacgcc
     1861 tccaacctgc ccggcgcggt cggtaaggcc gcccgcgagt ttgtgaaaag cctggaccgc
     1921 aagcagagat tcgatatgaa taagaacggc ctcgaaatta cagccaagcc cgttgccggc
     1981 gctgcggaag cgcaggcaga gccgtcctcc aaagctgcca ccaactgagc cgctgttgaa
     2041 cggcggagac agagccgagc ggctccccaa aacttactca aacaccagtg atgcgccaca
     2101 ctcttgtagc ggccaccttc aggtggcccc cgcatcatcc acatcatcgc cagaaaccat
     2161 ctccaaaagg tcaaggaggc ttccgtgaag tttctgtgcc tggtctacgg tgaagaaaaa
     2221 gaaatcgccg ccatgaccga cgatgagtgc atggattacg accgctcgct ccgcgacgct
     2281 ggccgctgcc tcgcttcgga ggctcttcag cccgttcgca ccgccgccag cgtgcgtctc
     2341 cgcaacggaa aagtgtccgt ccgcgacggc ccttttaccg aaacaaaaga aatcctggct
     2401 ggcttctacc tgatcgacgc ggccgacctg aacgatgcca tccaggtcgc ctccaagatg
     2461 ccgcccgctc gcgtcggcac catcgaagtc cgccccgtcc gcgagctgca aagcacctcg
     2521 ggcgaacgcc gccgcgttta gcccaccatc tttatctgca tgagaccagc ctattcccag
     2581 tgcgaccagg cgccgccttg ttcttcgtgc ttcttcctgc gcagcgcttc aatgcgcagg
     2641 cgctccgagc agtcgtcgca aatagttttt cggacgtcgc gccgcgcaca ttgcaaacgt
     2701 ggtgggcgtc ttctggtcgg ggatccggtc gctcgcgagg aactacttcc atgttggcct
     2761 cggctggcaa gctcgtggca gaggctcagg gcaagccacg ctccttgtaa tttcactctg
     2821 gtccaacgga ccgccaccag caaggttgtt cacgtgatac aggcgttctt gcctgtgtgc
     2881 cttcttcttc ccctgctgct tcagagcctt tgcgggtcgg agctttagct ccgacattag
     2941 cgcgcctaac gctaactgtg tcaccccaac gccccacgcc aaatttgtca gcccgaacga
     3001 agtgagggac ctgctttttc ttggtcgcac ttcgcttttc atcccgctgg tcctgaggag
     3061 tcgaaggaag ccgaagtgag caatctcttt ttcgcttccc atcgcgcccg gatttcaggt
     3121 ccagaagttg ttctcgccga acggaaactc cggcatttgt tcgctggcgg gactataatc
     3181 tcgaccagga ctccggccgc agcaacacac cgaacctgac gctgtgcgtt gtgccacttg
     3241 aaatctcaat tcgtgaatcc gctgacgtca ccatactaga cctgcgcggc agggcgacca
     3301 tcagcgacgg tgagagcgac ttgctcagag ccagtctgga tcagctcatc gccaatggcg
     3361 ctcgcaagct cgtgctcaat ctcgccgaac tcacccacgt ggatagttcc ggcttcagcg
     3421 tcatcgtcaa ggtatgcgtc tccgctcgcg acaagggtgg cgacttgcgc gtcgtacggc
     3481 cgtgtggtcc cgcgttggtg gccttcaacg tcctgcgcct gctcgacttg atccgcgcct
     3541 tcgacaacga agccgatgcg cttgccagct tccgccgcag cggttcctcc gcccagcctt
     3601 gagctccgtc tccggttctc tgtttgtttg cgcgccttag ctctaattca cctcgatcat
     3661 tgcagtccac aacagctttc catccaccgc aaacagactc ccctggggcc agggtcgttc
     3721 tgttttgccg ccggccggac gaccggcaac aaactgatca aacccacgcc gcgggcagcg
     3781 ctcgacctcg ccgcgcggcg tccaactttc tacttgacac aggtcacctt atacttcctc
     3841 gaactcattg cccacgccca cggagcaacc atgcctgact cactcgacga ctttcagcgc
     3901 actcgcgccg acctcaacga agagatcctc ggctgcggcc atctc
//
LOCUS       DASTPM010000435         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1197642, whole genome shotgun sequence.
ACCESSION   DASTPM010000435 DASTPM010000000
VERSION     DASTPM010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197642"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFR42_07650"
     CDS             complement(<1..291)
                     /locus_tag="VFR42_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369066.1"
                     /translation="MSSLPRLDLYLWAMTTLVALILLSLIFVRRLDRQFPFLTAYLGV
                     NLVQTVAQVFVYQFYGFDSDVTYASAWASQAVVVVARSLATGEFCYRVLGHYR"
     gene            complement(412..681)
                     /locus_tag="VFR42_07655"
     CDS             complement(412..681)
                     /locus_tag="VFR42_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369067.1"
                     /translation="MLQTRVALSLALLFGSIVVPGNKPVNVGAVHNDKNSARWADGSP
                     MPIPKPTGKAVQVADGSPMPLPKPNDGGSLVADGSPMPLPKPTGA"
     gene            768..1217
                     /locus_tag="VFR42_07660"
     CDS             768..1217
                     /locus_tag="VFR42_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369068.1"
                     /translation="MAVMDEILRIQPIVRDLTATTLAAIPNVFGRLTYIASLRDLSSG
                     RYEHSGLEAVYPRDAVQEALDFCHQEIFSRVLEMPLSLQEQDLGEFLEGMPGALPATI
                     VHWRQLEAYRILPPEDAPDYLKELFYSNLRALLEILESQHSTTRSGG"
     gene            complement(1195..1935)
                     /locus_tag="VFR42_07665"
     CDS             complement(1195..1935)
                     /locus_tag="VFR42_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369069.1"
                     /translation="MVPGLRLRQVRERLGLTYRDVEAASYELASRRGRSEFILHISRL
                     ADFENRNVVPGLHKLYSLAVLYHLNPLQLFAWYDVPFEECFEDGLQFLVPQTHVAAPP
                     SGLRVPLRFDPAFDPRRTDFLSRMVERWGHFEGALTKANPRHCYGYIGLADQRMAPLL
                     RPGSLVLVDTEMRQIENSGWNSEHDRPMYLVDIRKGYRCGWFVRDAEKLVMQPHPMSR
                     YAPESWVLPHEAEVIGKVIGVVTRLNES"
     gene            2104..>2423
                     /locus_tag="VFR42_07670"
     CDS             2104..>2423
                     /locus_tag="VFR42_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369070.1"
                     /translation="MITAPVPAQVFRPFFRSLLILSCLLAPVSLLAQEPSLKTTPPPA
                     NSPSDFAQAADEVLHNMSEITGLSLVSPLKKTLRSREEIRAYVIRQMDEDKDAAQRYA
                     DTKSA"
BASE COUNT          531 a          715 c          668 g          509 t
ORIGIN      
        1 gcgatagtgg cccagcaccc gataacaaaa ttcgccggtc gccaggctcc tggcgacgac
       61 cacaacagcc tgggaagccc acgcagatgc ataagtcaca tcggaatcga agccataaaa
      121 ctgatacaca aatacttgtg ccacggtttg cacaaggttc acaccgagat atgcggttag
      181 aaaagggaat tgccgatcca ggcgccgaac aaagattaga gacaagagga tgagcgcgac
      241 aagagtagtc atcgcccaca agtagagatc gaggcggggc agggagctca acacacgcgt
      301 atcttaccct ctctaagcga tataagaaag agataagata ccgcgtgcgt tattacgatc
      361 ctctaatacc tcaagggttt aatcaaagag ttcttattac aggctgttcg attacgcgcc
      421 cgttggcttc gggagcggca tcggcgatcc gtcggctacc agcgatccgc cgtcgttggg
      481 cttaggcaaa ggcatgggcg aaccatcagc tacctggacc gccttgccgg tcggttttgg
      541 gatcggcatg ggtgacccgt cggcccaccg ggcggagttt ttatcgttgt gaacagctcc
      601 gacgttgact ggcttgtttc cgggaactac gatggacccg aacagcaggg ctaaactcag
      661 cgcgacacgc gtttgcagca tggatgacac ctccaggcgc catccttcgg gctcgcattc
      721 gatcgctgga tttttagggc tgagattttc cggattcgga acagactgtg gcggtaatgg
      781 acgaaattct cagaattcaa ccgatcgtgc gggacttgac cgccaccacg ttggcggcga
      841 ttcccaacgt ttttggccgg ctcacttaca tcgcgtcact gcgtgatttg tcttctggac
      901 gttacgaaca ttccgggctg gaggcggtct atccgcggga cgcggtacag gaggccttag
      961 atttctgcca tcaggagatt ttttcgcggg ttttggagat gcctttgtcc ctgcaggagc
     1021 aggacctcgg cgaatttctg gaaggaatgc ccggcgcgct gcccgcgacg attgttcact
     1081 ggcggcaact ggaggcgtat cgcattctgc cgccggaaga cgcccccgat tacctcaaag
     1141 agcttttcta ttccaacctg cgtgccctgc tggaaattct tgagtcgcag cactctacga
     1201 ctcgttcagg cgggtaacca caccaatcac tttgccgatg acctcggctt cgtggggaag
     1261 cacccaggac tccggcgcgt agcgcgacat cggatgtggt tgcatgacca gtttttcggc
     1321 gtcgcgcaca aaccacccgc agcgatatcc cttgcggatg tccaccagat acatcggtcg
     1381 gtcgtgttcg gaattccagc cgctgttttc gatctggcgc atttcggtgt cgactagcac
     1441 caggctcccc ggcctcaaca acggcgccat acgttggtcc gccagcccga tgtacccgta
     1501 acagtggcgt ggattggctt tggtcagcgc gccctcaaag tggccccagc gttccaccat
     1561 tcgtgaaaga aaatcggtgc gccgcggatc aaaggccgga tcgaatcgta acggcacacg
     1621 cagtccgctg ggcggcgccg ccacgtgagt ctgcggcact aggaattgca aaccgtcttc
     1681 gaaacattct tcgaacggta cgtcgtacca agcgaaaagc tgcagcggat ttaaatggta
     1741 aaggactgcc agcgagtaca acttgtgcag gcctggaacg acgttgcggt tttcaaaatc
     1801 ggccagccgg ctgatgtgca aaatgaactc agagcgcccg cgccgcgagg caagttcgta
     1861 gctcgcggct tccacgtcgc ggtaggtcag ccccagtcgc tcacggactt ggcgcagacg
     1921 cagtcccgga accatagggt acctcaagga aggaaggaca actgcgagta gttgtaccat
     1981 accgttatta gtttgttaag gcagcataac agcctttagc cggagcgcgt ccctcaggca
     2041 ctcatgatcc gcctctcccc ggcgtcgcac tgagcatgcc gtgatatctg ctattctcaa
     2101 attatgatca ccgccccggt acctgcccag gtgtttcgcc cctttttcag gtcgttgctc
     2161 attttgtcct gcttgctggc cccggtgtcg ctgcttgcgc aagagccctc tctcaaaacc
     2221 acgccgcccc ccgccaactc acccagcgac ttcgctcagg ccgccgacga ggtgctgcac
     2281 aacatgagcg agatcaccgg cctttccctg gtttctccgc tcaaaaagac cctccgctcg
     2341 cgcgaagaaa tccgcgccta tgtgatccgt caaatggatg aagacaaaga cgctgcgcaa
     2401 cgctacgccg acaccaaatc cgc
//
LOCUS       DASTPM010000436         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_851530, whole genome shotgun sequence.
ACCESSION   DASTPM010000436 DASTPM010000000
VERSION     DASTPM010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851530"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..2022
                     /locus_tag="VFR42_07675"
     CDS             280..2022
                     /locus_tag="VFR42_07675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU0369071.1"
                     /translation="MADTIATNEAVLEVISPDGARRYARVVQTPYMIGRGAETGNHLQ
                     LNDRRISRQCAAIVVEANRFYIEDRGQRRGVFVNNEKVESRALQDGDIITFGLEDSYE
                     IVFRSSAEAGGETIPQLLTRIEHITSSEPSASATGGLRKLNMLLEASSLLHSQLPLDS
                     VLAKMLDHAVAVLDADRGLLLENGPDGTLKVRLARRSGGLRLPPESVTPSQTAIQLAL
                     KQHAPIITEDLAQAEADLQAAQSIVAQRLRAVVVIPLYSITRANSQESMVNLRRGDFL
                     GVLYLDSRRPAAFSKLDRQILDALAADAASTLDNARLVERERERQRMEQEINIARDIQ
                     QALLPKDFREFPHLAVSGCNFPCLSVGGDYFDVFPMSEGRTAFLIADVSGKGLGAALL
                     TTMLQGALSGMTLGNDPVKVFNHVNRFLCDHAEVGRYATMFFGVLDADGHMEFINAGH
                     PSPILIRKDSVEEAFTEGSYPVGLVPDAEYTAACLRLEPEDTLVLFSDGVTEAMDPDE
                     QLFGVPRLREVLADKKEMQLEDMQKSVLEAVENFTRGARQADDLTLLLIRYRSADVTA
                     TDTDLKASSASASD"
BASE COUNT          434 a          516 c          708 g          383 t
ORIGIN      
        1 ttgtttcgaa gagttgggca ggcagttaga cggcacgagc tagatccgcg cgcgctgtcc
       61 ggaacgtgga acgctgcttt cggaacttca agggcaagtc gatgaggagt tggcgagacg
      121 atgcaaaagc agatcggcgg atactggcag agcgagcgtt atcaaggaga tgggtggctg
      181 gagagctgcg gaatgaaagg gcgtatttag cggttttcag aatcgacaga tgcgaggagt
      241 gcgggacgcg cgtgctacaa tctgcggact gagacgctca tggccgatac catagccacc
      301 aacgaagcgg tactggaagt catttcgccg gatggggcgc ggcggtacgc ccgcgtagtg
      361 cagacgcctt atatgatcgg gcgcggggcg gaaaccggca atcacctgca actcaacgat
      421 cgcaggattt cgcggcagtg cgcggcgatc gtggtggaag cgaaccgatt ttacatcgag
      481 gaccgcgggc agcggcgcgg cgtttttgta aacaacgaaa aggtggagag ccgggcgctg
      541 caagacggcg acatcatcac tttcggactg gaagattcgt atgaaatcgt gtttcgttcg
      601 tcggcagaag cgggcggcga aacgattccg caactgctga cgcgaatcga gcacatcacg
      661 agttccgagc cgtcggcatc ggcgacgggt gggttgcgca agctgaacat gctgctggaa
      721 gcgagttccc tgctgcattc gcaattgccg ctggattctg tgctggcgaa aatgctggat
      781 cacgcggtgg cggtgctgga cgcggaccgc gggctgttgc tggaaaatgg ccccgacgga
      841 acgctgaagg ttcgtctggc gcggcggagc ggcggattgc gtttgccgcc ggagagtgtg
      901 acgccaagcc agacggcaat tcagctggcc ctgaagcagc acgcgccgat tattacagag
      961 gatctggcgc aggcagaagc ggacttacag gcggcacaga gcatcgtggc gcagagactg
     1021 cgcgccgtgg tggtgattcc actgtattcg atcacgcgcg cgaattcgca ggaatcgatg
     1081 gtgaatctgc ggcgcggcga ttttctcggc gtgctgtacc tggattcgcg gcgcccggcg
     1141 gcattctcga aactggatcg gcagattctg gacgcgctgg cggccgacgc ggccagcacg
     1201 ttggacaacg cacgactagt agagcgggag cgcgaacggc agcgcatgga gcaggaaatc
     1261 aacattgcgc gcgacatcca gcaggcgctg ctgccgaaag actttcgcga gttcccgcac
     1321 ctggcggtat ccggatgcaa cttcccgtgc ctatcggtgg gaggcgatta tttcgacgtg
     1381 ttcccgatga gcgaagggcg cacggcattt ctgattgcag acgtttcagg aaaaggattg
     1441 ggcgccgcgc tgctgacgac gatgctgcaa ggcgcccttt cgggaatgac gctaggcaac
     1501 gatccggtca aggtattcaa tcacgtgaac cggtttttat gcgaccacgc ggaagtgggc
     1561 agatacgcga cgatgttttt cggcgtactg gacgcagatg ggcacatgga gttcatcaac
     1621 gccggacacc cttcgccgat tttgattcgc aaggacagcg tggaagaggc gtttacggaa
     1681 ggatcgtatc cagtggggct tgttccagac gcggaatata ctgcggcttg cctgcgactg
     1741 gagccggagg acacgctggt cctgttcagc gatggcgtga cggaagcgat ggatccggac
     1801 gagcagcttt ttggcgtgcc gcggctgcgt gaagtgttgg cggacaagaa ggaaatgcag
     1861 ctggaagata tgcaaaagag cgtgctggaa gcggtggaga atttcacgcg aggcgcgcgg
     1921 caggcggacg accttacgct cttgctgatc cggtatcgct cggccgacgt gacggcgacg
     1981 gatacggact tgaaggcctc gtcggccagc gcgtctgact agggttttcc ccgaaagata
     2041 c
//
LOCUS       DASTPM010000437         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1226373, whole genome shotgun sequence.
ACCESSION   DASTPM010000437 DASTPM010000000
VERSION     DASTPM010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226373"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..777
                     /locus_tag="VFR42_07680"
     CDS             184..777
                     /locus_tag="VFR42_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369072.1"
                     /translation="MGYFEPFGKAAGKEMVFRIYGVECFLLGCGAILAISLLFVRKKY
                     SGVILSGALAWICLLILTYFQPLPTIDSWFSARAVASTVLRDSQMAADVRTLGLQRAW
                     QFGLNFYLHRPLREWDVSEPRGYVIASASGMEELNRKRLRFEVLNETSFQAFLIRVGG
                     SQNGPLNADVSGVRKETVEMMNRERLPSHPPGSGQPQ"
     gene            complement(739..1677)
                     /locus_tag="VFR42_07685"
     CDS             complement(739..1677)
                     /locus_tag="VFR42_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0369073.1"
                     /translation="MSILDEFKSPTQWYTRIVMGVLALLFFAVVATSAIAGFLVYRIV
                     KPQRSSSEINMDSFPGRPDAMKFSVPGAGQRDAWFFPGLVGAPTIVLCHGYGSSRGEL
                     LTLVSALQDHQYNVFVFDFVAHGKNDGITSFGYHEADEVRAAIDELARRNDVDPTQFG
                     LWGYNLGAYAALREAETDKRVRAMVLDSVYDSPEQMVKIGVEKEGLAGIPLMARSAEV
                     SFGWINHKYSGEKPLSWRLDSLAGVPKLFIEAADDPELAGITQKMFKNAPEPKEQVTI
                     LHGNFVNMDDADKRAYENRIVSYFLLRLPATGRMTR"
     gene            1880..>2635
                     /locus_tag="VFR42_07690"
     CDS             1880..>2635
                     /locus_tag="VFR42_07690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0369074.1"
                     /translation="MNILDLRQTSVRQLEPLLEEEARHWHDELHWDYRGALDLIKRFL
                     EAHALCGCVAFENGSAAGYSFYVLEEKKGLIGGLYVSPRYSQADIGRRLLEEMLFSMR
                     ALPQLSRIEAQLMPFGGPVDNPLREQGFKLYTRQFMLLDLHKDGESPAALAAGMRLDR
                     WNDRFFEPCAKLIYLSYANHVDGEINDQYRSRAGALRFLKNIILLPGCGQFVPGASLV
                     LRQPGSDELIGAVLTSEVSPGVGHTTQICVLPGY"
BASE COUNT          518 a          785 c          725 g          607 t
ORIGIN      
        1 gtcattttct ttaccgtctc gaagtccaaa ctcccaggct acatcctgcc gacaattccc
       61 gcgcttttgt tactcacggc tgttgctctg gccaagatgc tcagactgga aagggtttcg
      121 gttccgctgg gttttgcaat cattgcgctc acgccggtgg ttgccatcct agcgctcgct
      181 ctcttggggt attttgagcc ctttggcaaa gcagccggta aggagatggt gtttcgtata
      241 tacggcgttg aatgttttct acttgggtgc ggggcgattc tcgcgatctc gctgcttttt
      301 gttcgcaaaa aatactcggg agttattctg tcaggcgcgc tcgcctggat ttgccttttg
      361 attcttacgt attttcagcc actgccaacg atagactcat ggttctcagc gcgagccgta
      421 gcttcgaccg tgctgcgtga ctcccaaatg gcagcggacg ttagaactct gggcctgcaa
      481 agagcctggc aatttggctt gaacttttac ctgcatcgac cgctgcggga atgggatgtt
      541 tcggaaccgc ggggatatgt tattgcctca gcctccggaa tggaagaact gaacaggaag
      601 cgcttacggt tcgaggtgct gaacgagact tcctttcagg ccttcctaat tcgtgtcgga
      661 ggctcgcaaa acggtcctct taatgcggat gtgtccggag tgcgcaagga aacggtggag
      721 atgatgaacc gcgagagatt accgagtcat ccgcccggta gcgggcagcc gcaatagaaa
      781 atagctcacg atgcgattct cgtaagcgcg cttgtccgca tcatccatgt tcacaaaatt
      841 gccgtgcagg atggtcacct gctccttcgg ctccggcgcg ttcttgaaca ttttttgcgt
      901 gatgccggcc agttccgggt catcagcagc ctcaataaat aatttcggca ccccagccag
      961 cgaatcgagc ctccagctga gcggcttctc tccgctatat ttgtgattga tccagccgaa
     1021 actaacttcc gcggagcggg ccatcaacgg aatgcccgcc agcccttctt tctccacgcc
     1081 aatcttgacc atctgctcgg gcgaatcgta gaccgaatcg agaaccatcg cgcgcacgcg
     1141 cttgtcggtt tccgcttcgc gcagtgccgc gtaagcgccc aggttatagc cccacaggcc
     1201 gaattgcgtg gggtccacgt cgttgcgccg cgccagctca tcaatcgccg cgcgcacctc
     1261 gtcggcttcg tgatagccga aggaagtaat tccgtcgttc ttgccgtgcg ctacgaaatc
     1321 gaaaacaaac acgttgtact ggtgatcctg cagcgcggaa accagtgtca gcaattcgcc
     1381 gcggctcgaa ccatagccgt ggcacaacac gatggtcggc gctcccacta atccggggaa
     1441 aaaccacgcg tcccgctgcc ccgcgccggg aaccgaaaat ttcatggcgt cgggccggcc
     1501 cggaaagctg tccatattga tttcgctgct ggatcgctgc ggcttcacga tccggtagac
     1561 cagaaatccc gcaatcgccg aggtggccac caccgcaaag aacagcagcg ccaagacgcc
     1621 catcacaatt cgggtgtacc actgcgtcgg agatttgaat tcgtcaagaa tagacatggg
     1681 tgacctgcgc cactgggctg gcgcaaagcg ccgcttcgtt cctcactaca tcgttgtcat
     1741 cgtagcacgc ggtttttgcc gcttatttcg taactggccg ccatgtgtcg catgcagtag
     1801 cagctacccg ctccgggcct tcatttcgcg tgcttttctt gaatcggcta tcatacagtt
     1861 gcgcgctgcg ccgccttcga tgaacatcct tgaccttcgc caaaccagcg tccgccagct
     1921 tgagccgctg ctcgaggaag aagcgcggca ttggcatgat gagctgcact gggactatcg
     1981 cggcgcgctc gatctgatca aacgatttct ggaagcgcat gcgctgtgcg gctgcgtggc
     2041 ctttgaaaat ggctcggccg ccggatattc cttttatgtt ctggaggaga aaaaagggct
     2101 gatcggaggg ctgtacgttt ctccgcgcta ttcccaggcc gatattggcc ggcgcctgct
     2161 ggaagaaatg ctcttctcca tgcgcgcgct tccgcaactt tcgcgcatcg aagcgcagct
     2221 catgcccttt ggcggcccgg tcgataatcc gctgcgcgaa caaggattca aactctatac
     2281 ccggcaattc atgctgctcg atttgcacaa agatggagaa tcgccggcgg cactcgcggc
     2341 gggcatgcgc ctcgatcgct ggaacgatcg cttcttcgag ccctgcgcaa aactgattta
     2401 cttgtcgtac gccaatcacg tcgacggcga aattaacgac cagtatcgca gccgagcggg
     2461 ggcgctgcgc ttcctgaaaa acattattct gctgccggga tgcgggcagt tcgtgcccgg
     2521 cgcttccctg gtcctgcgcc agccgggcag cgatgagctc atcggcgcgg tgctcaccag
     2581 cgaagtttct cccggcgtcg gccacaccac ccagatctgc gtcctgcccg gctac
//
LOCUS       DASTPM010000438         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_966963, whole genome shotgun sequence.
ACCESSION   DASTPM010000438 DASTPM010000000
VERSION     DASTPM010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966963"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFR42_07695"
     CDS             <1..436
                     /locus_tag="VFR42_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0369075.1"
                     /translation="FEQIKLLTVTVDRLKTWARPGLVCIGDAAHAMSPIGGVGINLAI
                     QDAVAAGNLLFKPLQTRAPDDRELASVQTRREFPTRVTQRMQIFAQDNVISRVLASSH
                     IQAPWLLNLVDRWPFLQRIPARLVGMGFRPEHIRTPDVHASR"
     gene            511..1149
                     /locus_tag="VFR42_07700"
     CDS             511..1149
                     /locus_tag="VFR42_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369076.1"
                     /translation="MVCPICEKRKGARFCPAKGETICAVCCGTGREVTIDCEPDCTYL
                     LAAHRYEDEHPRTLPPDTPLLDVNLPSDVVHTQQQLMSALAFAIAKFCAAHPDATDPD
                     ILAAIQSLAETYKTLRTGIYYEKLPDTRLPRELYAALTTFIAEVKQQQAERPGSSAVK
                     DSDLFYLLVFLYRMGLLRTNGRPRARRYIEFLRSQFPEAQELEREESRIIVP"
     gene            complement(1181..2185)
                     /locus_tag="VFR42_07705"
     CDS             complement(1181..2185)
                     /locus_tag="VFR42_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0369077.1"
                     /translation="MVGIARLAADSPAAKTQRNSPERPEYFLLPVKSILNRCDSKRVP
                     FEWTINPYRGCEFACKYCYARYTHEYMELDGSEFEKKIFVKKDAAPLLVQDMRKYSFA
                     SKSSGGIYPEHIAIGTATDPYQPAEREYGVTRACLEELAKREGLSVSLITKSNQIVRD
                     IDLFKKIAERSELSLNITITTLRPRLARLLEPRAPRPDLRLAAVKQLRDAGLAVGVSA
                     CPLIPGITDRDGELESVAAAAKSAGAMWFFSNVLFLMPSAAKQFLPFVREKFPKLAKQ
                     YEEWYAKNGYAPEEYRKQMSQRIAKIRQECGFVSRPWEEKTRKTKQPQLSLLLQGSAA
                     "
BASE COUNT          456 a          842 c          665 g          526 t
ORIGIN      
        1 cttcgaacag atcaagcttc tcaccgtcac cgtggaccgc ctgaagactt gggcgcgccc
       61 cgggctggtc tgcattggag acgccgcaca tgccatgtcg cccatcggcg gcgtcggcat
      121 caatctggcg atccaggacg ccgtcgcggc tggaaatctt ctcttcaaac cgctgcaaac
      181 ccgtgcgccg gatgaccggg aacttgcttc cgtacaaacc cggcgcgaat ttcccacgcg
      241 ggttacacag cgcatgcaaa tcttcgctca agacaatgtc atcagtcgcg tgctcgccag
      301 ctcgcacatc caagcgccct ggctcttgaa tcttgtggat cgctggcctt ttttgcaacg
      361 tatacccgcc cggctcgtgg gcatgggttt ccgtcccgag cacataagaa cgcccgatgt
      421 tcacgcatcg cgatagggca cgcgcccgtt tttggctttt gcggtaacac ggcgtattct
      481 gctcttcgcg gctagctgcc ggagggctac gtggtttgtc ccatttgcga aaagcgtaag
      541 ggtgcgcgtt tctgtcccgc caagggcgag accatctgtg ctgtgtgctg cggcaccggc
      601 cgcgaagtca ccatcgattg cgagccagac tgcacctacc tgttggccgc ccatcgttac
      661 gaggacgagc atccgcgcac tctgccgccg gatactccct tgctcgacgt caatttgcct
      721 tccgacgtcg ttcataccca gcagcaactc atgtctgcgc tggccttcgc catcgccaag
      781 ttctgcgccg cccaccccga cgctaccgat cccgacatcc tcgctgctat ccagtccctg
      841 gccgaaacct ataagacctt gcgcactggc atctactacg aaaagctgcc ggacacccgc
      901 ctgccacgcg agctctacgc cgccctcacc acgttcatcg ccgaggtaaa gcagcagcaa
      961 gcggaacgcc ccggctcttc cgccgtcaag gattcagacc tcttctatct tctggttttt
     1021 ctgtatcgca tgggattgct tcgcaccaac ggccgccctc gcgcgcgccg ctatatcgaa
     1081 ttcctgcgca gccaattccc cgaagcccag gaactggagc gcgaagaatc ccgcatcatc
     1141 gtgccttgac catatcggcg ccgggccctc cttatgcacc tcacgccgcg ctgccctgca
     1201 aaagcagcga aagctgcggc tgctttgtct tccgcgtctt ctcctcccac ggccgcgaga
     1261 caaacccgca ttcctgccgg atcttggcta tccgttgcga catctgcttg cggtattcct
     1321 ccggcgcgta cccgtttttc gcgtaccact cttcatactg cttcgctaac ttcgggaact
     1381 tctcgcgcac gaatggcagg aattgtttcg ccgctgaagg catcaggaaa agtacgttcg
     1441 aaaagaacca catcgcgccg gcgctctttg ccgccgcggc cactgattcc agctccccgt
     1501 cgcggtccgt aatccccggg attagcgggc aggccgaaac gccaaccgcc agccccgcat
     1561 cccgcaactg cttcaccgcc gccagtcgca agtccggccg tggcgctcgc ggctccagca
     1621 gacgcgccaa tcttgggcgc agcgtcgtga tcgtgatatt cagcgaaagc tccgaccgct
     1681 ccgcaatttt cttgaacagg tcgatgtcgc gcacaatctg gttcgacttc gtgattagcg
     1741 aaacgctcag cccctcccgc tttgccagct cttccaggca cgcgcgcgtc acgccatact
     1801 cccgctcggc gggttgatac ggatcggtgg ccgtcccgat cgcgatgtgc tccggataaa
     1861 tcccacccga gcttttcgat gcaaacgaat acttccgcat gtcctgcacc agcagcggcg
     1921 ccgcgtcctt ctttacgaat atctttttct cgaattccga gccgtccagc tccatatatt
     1981 cgtgcgtgta ccgcgcgtag caatatttgc aggcgaactc gcatccgcga tacgggttga
     2041 tcgtccattc aaatggcact cgtttcgaat cgcaccggtt caggatggat ttcaccggca
     2101 acaggaaata ctccggccgc tctggtgaat tccgctgggt tttcgctgcg ggagaatcgg
     2161 ctgccagccg cgcgatgcca accaaaggcg cgggagcctc aggtgaaaaa cctgcggctc
     2221 ctccgccaca acgtgcggcg gcatctcggg tccggctttc atggcgttgg gcgccatgtt
     2281 cagccgagta cgccaggggg caatccggcg tggggcaacc cgagatgctg ccgctaggac
     2341 cagtcacgat cggggcaaga tcagatccgg tccgaactct tgggccttgt tccattcctt
     2401 gcgcgcaatc cgcggggaac agcgcggttt gtgtagcggg caaatgttgc gcatccgctc
     2461 gcaatctgga atgaactgga gacattggc
//
LOCUS       DASTPM010000439         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_44572, whole genome shotgun sequence.
ACCESSION   DASTPM010000439 DASTPM010000000
VERSION     DASTPM010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44572"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFR42_07710"
     CDS             <1..515
                     /locus_tag="VFR42_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0369078.1"
                     /translation="SFASAPLEHDLTITGPVSASLFASTSGTDSDFIVKLIDVFPDDA
                     QKNSWEPDEGPKPGQYAQSLNGYQLPIAMEVRRGRYLKSFEHPTPLKSNEPTQWEIPL
                     RDRDHVFLKGHRLMVQIQSTWFPLIDRNPQKFVPSIYQATAADFVSTTQRVYCSPKMS
                     SHIVLPVVTR"
     gene            522..971
                     /locus_tag="VFR42_07715"
     CDS             522..971
                     /locus_tag="VFR42_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU0369079.1"
                     /translation="MPTEYTSMLDVAIAEARKGLAEGGIPIGAAIFDETGKLVGAGRN
                     RRVQKGDPSLHGETDAFRNAGRQRSYRKLTMVTTLAPCWYCSGLIRQFGFARVVVGES
                     RTFQGGIDWLRSLGIEVVDLDSQECVELLTAYIQKNSDVWNEDIGEE"
     gene            complement(991..2286)
                     /locus_tag="VFR42_07720"
     CDS             complement(991..2286)
                     /locus_tag="VFR42_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0369080.1"
                     /translation="MARPPSPLAELRAKLLTATVTAQESASAALHNTNTNASRNVYLA
                     LDREWTLREAARAEQAFRAAEKPPLFGLPVSLKDCFDLQGFKTTAGTKFYAERNQNAA
                     ADSAVAARLREQGIVIIGKTHLHPLAYGITGENRDFGDCVQPRDASLLTGGSTSGGAA
                     SVQEGSAVAAIGTDTGGSIRVPAALCGLAGYRASLELARDRGLWTGGVHLSQTFDTLG
                     WIFRDLRDGPLLAEGLFGVRVAEKCSPRVRVGALAGDFLRDCDACVAQAFAECKNQLA
                     AVGAEIGAFEADHWDEGTSIYAPIQASEAAAIHAPHTGGEFSCFPAPISERLAWGASL
                     SPEEIQQQRKRHAEFREQVDKLFDRFDFLLLPCSPISLLKAGVDHSETRMKILRYTVP
                     MSLAGTPVVTLPFAGGAGMQLVAPRGADAKLLAYAAAYC"
     gene            complement(2287..>2554)
                     /locus_tag="VFR42_07725"
     CDS             complement(2287..>2554)
                     /locus_tag="VFR42_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369081.1"
                     /translation="HYCSEGNQPSMKTESLKAKANKYSFSLVSVSGLKSPDAGHMVGL
                     VLTLVDKDHLKQDWTYTDKGKTSTNSFEFRRRPEKAATVTPTGH"
BASE COUNT          490 a          815 c          726 g          523 t
ORIGIN      
        1 tttctttcgc cagcgctccg ctcgaacacg atctcaccat caccggcccg gtctccgctt
       61 ccttgttcgc ttctacttcc ggcacggaca gcgacttcat cgtaaaactc atcgacgttt
      121 ttcccgatga cgcgcaaaag aatagttggg agccggacga aggcccgaag cccggccagt
      181 acgcgcaatc cttaaacggc taccagctac cgatcgccat ggaagtccgc cgcggtcgct
      241 acctgaaaag tttcgagcat cccacgccgc tcaaatccaa tgagcccacg caatgggaaa
      301 ttccgctgcg tgatcgcgat cacgttttct tgaaagggca tcgcctgatg gtccagattc
      361 aatccacctg gttcccactg atcgaccgca acccgcaaaa attcgtgccc agcatctatc
      421 aagcaaccgc tgccgatttc gtctctacca cccagcgcgt ttactgttcg ccaaaaatgt
      481 catcgcacat cgttttgcct gtcgtcacca ggtgagtccc gatgccaacc gaatacactt
      541 ccatgctgga cgtggcgata gcggaggcac gcaagggcct cgccgagggc ggcatcccga
      601 tcggtgcggc gatcttcgac gaaaccggaa agctggtagg ggcgggacgc aatcgccggg
      661 tgcagaaagg cgatccctcc ctgcatggcg agaccgacgc cttccgcaat gccggacgac
      721 agcgcagtta tcgcaagctc accatggtta cgacgctcgc tccgtgctgg tattgcagcg
      781 gactcattcg ccaattcgga ttcgcccgcg ttgttgtcgg tgagagccgc acgtttcagg
      841 gcggtatcga ctggcttcgc tcgctgggca tcgaagtggt cgatttggat tcacaggaat
      901 gcgtcgaact cttgaccgcg tacattcaaa aaaactccga cgtctggaac gaagacatcg
      961 gcgaggagta gagacaacgc gcagaaaaat tcagcagtat gccgcagcgt atgccagcag
     1021 cttggcgtcc gcaccgcgcg gagctaccag ctgcatgccc gcaccgcccg cgaacggcaa
     1081 cgtaaccacc ggcgttcccg ccaggctcat gggcacggtg tagcgcagaa ttttcatccg
     1141 cgtttccgaa tgatccacgc cggctttcag caggctgatc ggcgagcatg gcagcagcag
     1201 aaaatcgaac cggtcaaaga gtttgtccac ttgctcgcga aattccgcgt gccgttttcg
     1261 ctgctgctga atttcttccg gtgaaagaga cgcgccccag gccagccgct cgctgatcgg
     1321 cgcaggaaag caggaaaact cgccgcccgt gtgtggcgcg tggatcgccg cagcttcgct
     1381 cgcctgaatt ggcgcgtaga tggacgtgcc ctcgtcccag tggtccgctt caaacgcgcc
     1441 gatttccgcg cccaccgcgg cgagttggtt cttgcactcc gcaaacgctt gcgcaacgca
     1501 tgcgtcgcag tcgcgcagaa aatctccagc cagcgcgccg acacgaacgc gcggtgaaca
     1561 tttctcagct accctcactc caaacagtcc ttccgccaac agcggtccat cgcgcaagtc
     1621 ccggaagatc cagcccaatg tgtcgaatgt ctgcgacaaa tggactccgc cggtccatag
     1681 gccgcgatcc cgcgcgagtt ccaggctggc gcgatagccg gcgaggccgc aaagcgcggc
     1741 gggaactcgg attgacccgc ccgtgtccgt cccgattgcc gcgacggctg agccctcctg
     1801 tacgctggcg gctccgccgc tcgtcgatcc tccggtcagc agcgatgcat cgcgcggctg
     1861 cacgcagtcg ccgaagtcgc gattctctcc ggtgattccg taggccagcg gatgcaggtg
     1921 cgttttgccg atgatgacaa tgccttgctc gcgcaagcgc gcggcgactg ccgagtccgc
     1981 cgcggcgttc tgattcctct ctgcgtagaa cttcgttcct gctgtggtct tgaatccttg
     2041 caggtcaaaa caatccttca gcgaaactgg caagccgaac agcggcggct tctcggctgc
     2101 gcggaacgct tgctccgcgc gcgctgcctc gcgcaacgtc cactcgcgat ccagcgcgag
     2161 atacacattg cgcgaagcat tggtattggt gttgtgcagc gctgcgcttg ctgattcctg
     2221 tgctgtgacc gtcgcggtca gaagtttcgc gcgcagctcg gccagtggac ttggcggccg
     2281 cgccactcag tgtccggtcg gcgtcacggt cgccgctttt tccggcctgc ggcgaaactc
     2341 aaaggagttg gtgctcgtct tgcctttgtc ggtgtaggtc cagtcctgct tcaagtgatc
     2401 tttgtcaact aaggtgagga cgaggccgac catgtgcccg gcatcgggtg acttcaatcc
     2461 ggaaacgctc accagactga atgagtattt gttggctttc gctttcaggc tttccgtctt
     2521 catggacggc tgatttcctt cgctgcagta gtgg
//
LOCUS       DASTPM010000440         5010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1428602, whole genome shotgun sequence.
ACCESSION   DASTPM010000440 DASTPM010000000
VERSION     DASTPM010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428602"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFR42_07730"
     CDS             complement(<1..693)
                     /locus_tag="VFR42_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369082.1"
                     /translation="MRKSAHTLLLSIIVLIAALTITACGGSSASKPPIDPSGNWAMKF
                     SDASGNSFIMSSLFSQSGSVVTALNTLAAGNPAPFSCVPFTASFANGQVLNVNQFFGD
                     INTPFGNIHFTSTLNPDGSHADGNYTLTGNCWTVAPTGTFTADEVPSVSGSWTGTITC
                     TLNCPAGATTGTIAAALTQNDATGLVSGTYVVTGLPNISSGTIATRTGIDVLSGESWQ
                     DGATDNNGNFYVF"
     gene            complement(690..959)
                     /locus_tag="VFR42_07735"
     CDS             complement(690..959)
                     /locus_tag="VFR42_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369083.1"
                     /translation="MNQCPYRIKDALAIGWFNCQSEAGHSGDHVPPLPQVGRKNRFEG
                     IWHRPGEKSTADLEPHVPSVPSVWAAKGSEEITPSNQHRKEEVKK"
     gene            complement(1134..1838)
                     /locus_tag="VFR42_07740"
     CDS             complement(1134..1838)
                     /locus_tag="VFR42_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369084.1"
                     /translation="MMKGQPNNPVTCEKCGGPDGFPNVRRCRRCMPPTSRKKHYWTPD
                     LDERLRRIYAEHAHSRDDLGMAVTSLAKHMRCPRYIVFNRAGELRLRTSRGKPWTKED
                     IQFLRESAGVKSIEFMMRTLKRGQVALVSKLWELRLSWRVTEGYSRQDLAAVFCVSPY
                     TVGKWIDRGWLRPLRSTDRIPEDQVLRFIKTHPEEYSLKRVDEAWFKGMLFPGFGVSV
                     HARREHAAVLGVEHHA"
     gene            complement(1835..2149)
                     /locus_tag="VFR42_07745"
     CDS             complement(1835..2149)
                     /locus_tag="VFR42_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369085.1"
                     /translation="MNTLSMNRAIASTFPDPEAVLHRIDPLDRITIGELPLSLRDDAL
                     QVLRSFQRETINALADRLVDAAARLEQTNNHVTFDDLKREAQALRDYAETLAPATPEV
                     NR"
     gene            complement(2146..2361)
                     /locus_tag="VFR42_07750"
     CDS             complement(2146..2361)
                     /locus_tag="VFR42_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369086.1"
                     /translation="MSLKVTIMQPKRKAKIAYQAVLHSLEEQMSELGDFGDDEEVLLT
                     APLGATEEQVDRAMAELGCSPDEAVKQ"
     gene            complement(2358..2849)
                     /locus_tag="VFR42_07755"
     CDS             complement(2358..2849)
                     /locus_tag="VFR42_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369087.1"
                     /translation="MNDEQQLGFAGMGLEPTPDTHVRNVMLGRSGPWQPTKLQRELLR
                     ILLFHQGAQSAVPLHVLMVKLDSVCKPTPTERQIKDAARSLVVDFKVRIGASRGEPVG
                     YFLVTTAEEARDAASPYISEIRQLARRVRVLLDPHDLGELQGQRWIENLLAEPDGDPK
                     EAA"
     gene            complement(2850..3053)
                     /locus_tag="VFR42_07760"
     CDS             complement(2850..3053)
                     /locus_tag="VFR42_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369088.1"
                     /translation="MEQALSEFNTAVSTLATSSTNLSVANSNLLAAKEKLKAAIDTAA
                     SAAQTSAAGTASAIAPQPQTAAA"
     gene            complement(3034..3294)
                     /locus_tag="VFR42_07765"
     CDS             complement(3034..3294)
                     /locus_tag="VFR42_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369089.1"
                     /translation="MRPDFIISERVMDGVHVYIVLPTHTLAMIWMIARVTEQERHSLS
                     AHLAHERLVEFQGDAALEGMTFSQPGSPQPAGGESTHGTSTQ"
     gene            complement(3443..4351)
                     /locus_tag="VFR42_07770"
     CDS             complement(3443..4351)
                     /locus_tag="VFR42_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369090.1"
                     /translation="MANCAPCLKLGLQVVAHYDADLRAGKEAECYYHHFGMTLPASLQ
                     EKIAKPKPQPETEAEETGAPILEKTKPRPDFGVNSRPGLNPDVCAECWRNGHVCKPHC
                     TVDGERLCIDCADGKPCAHVRAKEENPQARPHERYRVTIPSEKKAEAPRPTITTIPAN
                     ISLSLDAKGVPARAVSATRTPPSKPAPEPEVPTAGKDRQKKERTHMKLTDEQRAQQRT
                     CPFCKMPLRRDNTKGICTSCRKKGKRLGDEHASSAPAPIKTDATPEPESGLIALKVTE
                     AHLDNFLVKLPLEEKIAIVQKQLAGA"
     gene            complement(4355..4588)
                     /locus_tag="VFR42_07775"
     CDS             complement(4355..4588)
                     /locus_tag="VFR42_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369091.1"
                     /translation="MITSGTNSILFDQGAVLFISSVPGRCACQRTAFLFVGRNGRTHC
                     LECEAKRLMVAPSECDPTDDGPDAAAAFQKAIV"
     gene            complement(4589..4960)
                     /locus_tag="VFR42_07780"
     CDS             complement(4589..4960)
                     /locus_tag="VFR42_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369092.1"
                     /translation="MTRAQINIALLVIAGMFLRDAWCWSVAKFFCWRKQRQTQRRVKQ
                     DRELRALQSQLVSVEELEKDARIAKVMLAASKVPYPQLLAGPLIVKARCNHEVLLITK
                     QMQRNFAAQGKQIVDGNFEDN"
BASE COUNT          885 a         1445 c         1551 g         1129 t
ORIGIN      
        1 aaaaacatag aaattgccgt tgttgtcagt cgcgccgtcc tgccatgact cgccgctgag
       61 cacgtcgatt ccagttcgcg tcgcgatcgt gccgctgctg atgttcggca ggccggtgac
      121 gacgtacgtg cctgagacaa gcccggtggc atcgttctga gtcaatgctg cggcgatcgt
      181 gccagtggtg gctccggccg ggcaattgag tgtgcaggtg attgtgccgg tccacgatcc
      241 ggagacgctg ggcacttcat cggcggtgaa ggtccccgtg ggcgcgacgg tccagcagtt
      301 tcccgtcaga gtgtagttcc catcggcatg cgagccgtct ggattcagcg tgctggtgaa
      361 gtggatgttt ccgaacggcg tgttgatgtc gccgaagaac tgattcacgt tgagcacctg
      421 tccgttggca aagctggcgg taaagggcac gcaggaaaac ggcgcgggat tgccggccgc
      481 gagcgtgttg agcgcggtga cgaccgagcc gctctgactg aagagcgatg acatgatgaa
      541 actgttgccg ctggcgtcag agaatttcat cgcccagttg cctgaaggat cgatcggcgg
      601 ctttgacgcg gaactgcctc cgcaggctgt gatggtgagc gcggctatca gaacgatgat
      661 cgacagcagc agggtgtgtg cggactttct catttcttta cttcctcttt tctgtgttga
      721 ttggaggggg tgatttcttc agaacctttg gccgcccaca cggacggaac actgggaacg
      781 tgcggctcaa gatcggcggt gctcttctct cctggccggt gccagatgcc ttcaaacctg
      841 tttttgcggc cgacctgagg aagcggtgga acgtggtcgc cgctgtggcc ggcttcgctc
      901 tggcagttaa accagccgat ggcgagcgcg tctttgatgc ggtaggggca ctgattcatg
      961 ctgcggtctc cttggtcttc cagagcaatt cgaacaacga cttccgcttg cacctggtgc
     1021 acatccacgt tgccagcagt tccagtggac gcggcctaag acaaaagaca cagcacgcag
     1081 tgggatcgtg atctgcgaac agggtagccg gaatttcgcc ttttgcagca gagctaagca
     1141 tgatgttcca ctcccagaac tgccgcgtgc tcgcggcgtg catggacgct gacgccgaag
     1201 ccgggaaaca gcattccctt aaaccacgcc tcgtcgaccc gtttcaggct gtactcttcc
     1261 gggtgagttt tgatgaagcg cagaacctga tcttcaggaa tgcgatcagt cgagcgcagc
     1321 ggccgcagcc atcctcggtc gatccacttg ccgaccgtat agggggaaac gcagaacact
     1381 gcggccagat cctgccgaga gtacccttcc gtcacacgcc agctcaggcg cagctcccac
     1441 agtttggata caagagctac ctgcccccgc ttcagcgtcc tcatcatgaa ctcgatagac
     1501 ttgacgccgg cgctctcgcg caggaactgg atatcctctt tagtccacgg cttaccacgg
     1561 ctggtgcgaa ggcgaagctc tccggcgcga ttgaacacga tatatctagg gcagcgcatg
     1621 tgctttgcca gagatgtaac cgccatccca agatcgtccc tgctgtgagc gtgctcggcg
     1681 tagatgcggc ggagtctctc gtccaggtca ggagtccagt aatgcttctt gcgcgaagtt
     1741 ggcggcatgc agcgccggca tcggcggaca ttgggaaaac cgtcaggccc accgcacttc
     1801 tcgcatgtca ccggattgtt cggctggcct ttcatcaccg attgacctcc ggcgtcgccg
     1861 gcgcgagggt ttcggcatag tcgcgcagcg cctgagcttc gcgcttgaga tcgtcgaacg
     1921 tgacatgatt gttcgtttgt tccaggcgtg cggcggcgtc aaccaggcgg tcggccagcg
     1981 cgttgatggt ctcgcgctga aaggagcgca gcacctgcaa ggcatcatcg cggagactaa
     2041 gaggcaactc acctatcgtg atgcggtcaa gcggatcaat acgatgaagt actgcctcgg
     2101 gatcggggaa cgtcgaagcg atggcgcgat tcatggagag ggtgttcatt gcttgaccgc
     2161 ctcgtcgggt gaacagccaa gctccgccat tgcgcggtct acctgctcct cagtcgcgcc
     2221 tagtggagcg gtcagaagaa cctcctcgtc gtcgccgaaa tcgccgagct ccgacatttg
     2281 ttcttccaag ctgtgcagaa cggcctggta agcaattttc gccttcctct ttggttgcat
     2341 gatcgtcact tttaagctca cgctgcctcc ttgggatcgc cgtctggttc ggccagcagg
     2401 ttctcaatcc agcgctggcc ctggagctcg ccaaggtcat gcgggtcaag caggacacgc
     2461 acgcgccggg cgagctggcg gatctccgat atataaggtg aagctgcatc ccgcgcctcc
     2521 tcggcagtgg ttacgaggaa atagccaaca ggctcgcccc gtgatgcgcc gatgcggact
     2581 ttgaagtcca cgaccaaaga ccttgcggca tctttgatct ggcgctcagt cggagtgggt
     2641 ttacagacgc tgtccagctt caccatcagg acgtgcagag gaacggcgct ctgcgcgccc
     2701 tgatggaaaa gcagaatgcg aagcagctcg cgctgcagct ttgtcggctg ccagggaccg
     2761 gagcgcccga gcatcacgtt gcgaacgtgc gtatcgggcg ttggctccag tcccatgccg
     2821 gcaaaaccga gttgttgctc gtcgttcatt taggcggcag cggtctgcgg ctgcggagcg
     2881 atggccgaag cggtaccggc tgcgctggtc tgtgccgcac tggcggcggt gtcgatggct
     2941 gctttcagct tttctttcgc ggccagcagg ttgctgttgg ccacgctgag gttggtggac
     3001 gatgtcgcca gtgtgctgac ggcggtgttg aattcactga gtgcttgttc catgtgtgct
     3061 ttctcctccc gctggttgcg gggaacctgg ttgtgaaaag gtcatgcctt cgagtgcggc
     3121 gtcgccctga aattcgacca ggcgctcatg cgccaggtgc gcggagagac tgtggcgctc
     3181 ctgctccgtg acccgcgcga tcatccaaat catcgcgagc gtgtgcgtgg gaaggacgat
     3241 gtaaacgtgg acaccgtcca taactcgttc gctgatgatg aaatccggtc tcacgcttgc
     3301 ctcacttctg tcatcgttct gtacatctcg gttagccagt ccatctctcc aattgctgcc
     3361 agccagttgc ccggcgcttg ctggctgcgc gcgaatacga tcattgacgc ggcgtgcaag
     3421 atgttctgga ggggatcgtc gatcacgcgc cagcaagctg cttctgcacg attgcgatct
     3481 tttcttccag tggcagcttt accaggaagt tatcgaggtg cgcctcggtg actttgagag
     3541 caatcaggcc actctccggc tcaggtgtgg catcggtttt tataggcgca ggcgcggaag
     3601 atgcatgttc gtcgccgagc cgtttgccct tttttcggca gctagtgcat atcccttttg
     3661 tgttgtcgcg gcgcagcggc attttgcaaa atgggcatgt cctctgctgc gcgcgttgtt
     3721 cgtcggtaag tttcatgtgt gtcctctcct ttttctgccg gtcctttcct gctgttggaa
     3781 cttcaggctc tggcgctggc ttcgacggcg gcgtgcgcgt tgcgctcacc gcgcgcgcag
     3841 gaacgccttt ggcatcgagc gaaagtgaga tgttggcggg aatggttgtg atcgttggcc
     3901 ttggcgcttc cgccttcttc tcggacggga tcgtcacgcg atagcgctca tgcggacgcg
     3961 cctgcgggtt ctcttctttg gcgcggacgt gcgcgcaggg cttgccatcg gcgcagtcga
     4021 tacagaggcg ctcgccgtcg acggtgcaat gcggcttgca aacgtggccg ttccgccaac
     4081 actctgcgca cacgtcggga ttcagccctg ggcgtgaatt cacgccaaaa tcaggtcgcg
     4141 gctttgtctt ttcaagtatc ggtgcgccgg tctcttccgc ctcggtctcc ggttgcggct
     4201 tgggctttgc gatcttctcc tggagagaag caggcagcgt catgccgaag tggtggtagt
     4261 agcactcggc ttctttgccg gctctgagat cggcgtcgta gtgggcgaca acctggagtc
     4321 cgagtttcag gcaaggtgcg cagttcgcca tggcctacac gatcgctttc tggaacgctg
     4381 cggccgcgtc cggtccgtca tcggttggat cacactcgct gggcgcgacc atcaggcgct
     4441 tcgcttcgca ttcaagacaa tgcgtgcggc cgttgcgccc gacgaacagg aacgccgtgc
     4501 gctggcacgc gcagcggccg ggcacgctgg agatgaacag cactgctcct tggtcaaaaa
     4561 ggatcgagtt ggtaccggag gtgatcattt agttgtcctc aaagttgccg tcgacgattt
     4621 gtttgccctg ggctgcgaag ttcctctgca tctgcttggt aatgagtaga acttcatggt
     4681 tgcaacgcgc cttgacgatc agcggaccgg caagcagctg cggataagga accttggacg
     4741 cggccagcat gaccttcgcg atccgcgcgt ccttctccag ctcctcaacg ctcaccagct
     4801 gggactggag cgcacgcagt tcccggtcct gcttcacgcg gcgctgcgtc tggcgttgtt
     4861 tgcgccagca gaagaacttc gcaacactcc agcaccaggc gtcgcgcagg aacatgccgg
     4921 cgataacaag gagagcgatg ttgatctgcg cgcgagtcat gcgttcactc tttccagcag
     4981 gtgatcgctc acatcgccga gatcttcatc
//
LOCUS       DASTPM010000441         5569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_736489, whole genome shotgun sequence.
ACCESSION   DASTPM010000441 DASTPM010000000
VERSION     DASTPM010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736489"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1770)
                     /locus_tag="VFR42_07785"
     CDS             complement(<1..1770)
                     /locus_tag="VFR42_07785"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0369093.1"
                     /translation="MQNRLWPLRLTAAALVASLALPANRLAASDTPADDSDSAKSSAA
                     PSPDPVMQVMQGELSRATSALAKSDPAPYYLSYTVYDQHVIVLVGSYGSLLTDSALHR
                     RQADVTMRIGSPALDNTHGQSRPSGMVSGVLPYGNDPNSLSRVLWELTDRAYKRSSPA
                     FLNVKTNTAVRAEEEDKSPDFSRESPVTHTTEDVKLPDFDRPTWEAEIRRISSVFRNY
                     PNVYYANVVLQVQDAKQRKVTSEGTAIITPSVSTRLVMEAQTRADDGMDLLRVETFQA
                     PSARALPDEKELSAKAEKMAADLDDLRKAPVAQPYDGPALLSGRAAAVFFHEVLGHRL
                     EGHRQRDEDEGQTFTKKVGQEVLPKFLSITDDPTKKEVNGIKLAGNYEYDDEGVPAQR
                     VEVITDGVLKNFLMSRMPIKNFDNSNGHGRNQPGLMPTGRQGNLIVTSSQTVPEKDMR
                     QKLIDEIKKQNKPYGLYFDDIQGGFTLTTRQLPQAFQVLPVIVYKVYADGRPDELVRG
                     VDIVGTPLAALTRIITTSDEQHVFNGVCGAESGSVPVSAVAPAMLFSEMEVQKRGHEH
                     DRPPILPPPGFENQPAKTATPAGE"
     gene            complement(1853..2791)
                     /gene="fmt"
                     /locus_tag="VFR42_07790"
     CDS             complement(1853..2791)
                     /gene="fmt"
                     /locus_tag="VFR42_07790"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU0369094.1"
                     /translation="MRLLFCGTPSFAVPTLKHLLAQSDFQIAAVITQPDRPRGRGHDL
                     SFSPVKEVALAANIPVFQPEKIRTLESEHFLREADPDAIVIIAYGQIIPERLLNIPRL
                     GWINLHASLLPKYRGAAPIQWAIANGESATGITTMRIDAGMDTGDILLQRETPIGPTE
                     TTPQLAERLSELGAPLMAETLRGLRDNTLKLEPQDHSAATVAPLLKKESGQINWQQPA
                     QVIYNRMRGFTAWPGAYTHFRATTCHLLGQPASDSFSAGAPGQILYDGKELLVACGQQ
                     SFLYVSHVKPEGRKQVSAVDFANGVHLRSGEYFGDA"
     gene            complement(2931..3521)
                     /gene="def"
                     /locus_tag="VFR42_07795"
     CDS             complement(2931..3521)
                     /gene="def"
                     /locus_tag="VFR42_07795"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0369095.1"
                     /translation="MPDKSTTSEETIAAANAAAPEVSAPPRKIYPIVKYGDPILEKPA
                     KTIAQFDAELEELAEDMFASMYAAQGVGLAAPQIGKSIRLAVVDVTSGKNPEAKIVLV
                     NPEVIHGEGEKREEEGCLSIPGFRGYVVRPQFVTIKAQNLKGEPFEIRGEDLLARAFC
                     HEIDHLNGILFLAHLSMLKRDLIKRKIKKLKKQGEW"
     gene            complement(3496..4668)
                     /gene="aroC"
                     /locus_tag="VFR42_07800"
     CDS             complement(3496..4668)
                     /gene="aroC"
                     /locus_tag="VFR42_07800"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU0369096.1"
                     /translation="MLKFYTAGESHGQALLSFLSGLPAGLPIDLEFINRDLHRRQLGY
                     GRGGRQKIEKDRADIFAGVRHGQTIGAPIALRIENRDWANWEKILAVEAYPEDDSTAK
                     KLVAPRPGHADLAGSQKFNFHDARYILERASARETAARVAAGALAKQLLRAFAIEIAS
                     HTIQVGHTRADRSPSWEEILAISNDLESPLRCVDSAAQDKMKAEVDSVLKAGDTVGGV
                     FEIVVHNVPVGLGSHAQWDEKLDGRLAQAVMSVQAVKGVEIGAGLLAAGSFGSEVQDE
                     ISYDESTHKFSRSSNRAGGLEGGITNGEDVVVRGYLKPISTLRRALKTADMVTKQPVQ
                     AAYERSDWCVVPAGGVAGEAMVALVLADAFLQKFGGDSLTETRRNFEGYARQIHDF"
     gene            4917..>5569
                     /locus_tag="VFR42_07805"
     CDS             4917..>5569
                     /locus_tag="VFR42_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369097.1"
                     /translation="MAPTASTPLIPEPAPQKTALVVDSTPAVNAMLTRVLKDQGIDIR
                     RAVDNKTVLSLVKESPFDLIITGQKTCGREDVALLHAIRNVRPNVRMIILADESTPAD
                     VIDAVRGGAFSYFCPPYTRGGFVEMVHLAMTQPAWDDGIEIVSATTSWVRLIVRCEVA
                     TADRLVQFLRAGSTLPEGEKEDVISAFHEILMNAIEHGAHFDPSQYTEVAFVRARRA"
BASE COUNT         1096 a         1379 c         1894 g         1200 t
ORIGIN      
        1 ctcgcccgcg ggagtagcag tttttgccgg ctgattttcg aagccgggcg gaggcagaat
       61 cggcggacga tcgtgttcgt ggccgcgctt ttgcacttcc atttcagaga aaagcattgc
      121 cggagctacg gcggaaaccg gaacgctgcc gctttccgca ccgcagacac cgttgaagac
      181 gtgctgctcg tcgctggtgg taatgattcg ggtcagcgcc gccagcggcg tgccaacgat
      241 gtcgacgccg cgaacgagtt cgtcgggacg gccgtcggcg tagaccttat acacgatgac
      301 cgggagcacc tgaaacgcct gcggaagctg tcgcgtggta agcgtgaagc cgccctgaat
      361 atcatcgaaa tacaaaccat aaggtttgtt ttgttttttg atttcgtcaa tgagtttttg
      421 acgcatgtct ttttcgggaa cggtttgtga tgaggtcacg atgagatttc cctggcggcc
      481 tgtcggcatg agtcccggct gattgcggcc gtggccgttg gagttgtcga aattcttgat
      541 aggcatgcgc gacatcagaa aatttttcag cacgccgtcg gtgatgactt caactcgctg
      601 agcgggaaca ccttcgtcgt cgtattcgta attgccggcg agcttgattc cgttgacttc
      661 ttttttggtg ggatcgtcgg tgatggaaag aaatttggga agaacttcct gaccgacttt
      721 tttcgtaaag gtctggcctt cgtcttcgtc gcgctgacga tggccttcca ggcgatgacc
      781 gagaacttca tgaaaaaaaa cggcggcggc gcgcccgctg agtagcgcgg gaccgtcgta
      841 cggctgagcc acgggcgctt tgcggaggtc gtcgagatcg gcggccattt tttcggcttt
      901 agcactgagt tctttttcgt cgggcagagc tctggcggaa ggcgcctgaa atgtttcgac
      961 gcggagaaga tccatgccat catcggcgcg cgtttgcgct tccatcacca atcgcgtgga
     1021 aacgctgggg gtaatgatgg cggtgccttc gctggtaact ttgcgctgct tggcgtcctg
     1081 cacctgcaac accacgttcg cgtaatagac gttgggatag ttgcggaaga cggaagagat
     1141 gcggcgaatt tcggcttccc aagtggggcg atcgaaatcg gggagcttga cgtcttcagt
     1201 ggtgtgcgtg accggcgatt ctcttgagaa gtcaggcgat ttgtcttctt cttcggcgcg
     1261 aacggcagtg ttggttttga cgttaaggaa agccggtgag gaacgtttgt aggcgcgatc
     1321 ggtgagttcc cacagaacgc gagagagcga gttgggatca ttgccgtagg gaagtacgcc
     1381 ggagaccatg ccactggggc ggctttggcc gtgcgtattg tcgagggcgg gagaaccgat
     1441 gcgcatggtg acgtcggcct ggcggcgatg cagcgcggaa tcagtgagca gcgagccgta
     1501 ggagccgacg agcacgatga catgctggtc gtagacggtg tagctcaagt agtacggcgc
     1561 gggatcggac ttggcgagcg ccgacgtggc gcgggagagt tccccttgca tgacctgcat
     1621 caccggatcg ggagaaggcg cggcggagga ttttgcggag tcggagtcgt cggcgggcgt
     1681 atccgaggcc gcgaggcggt ttgccggaag ggctagcgag gcgacaagcg ctgcagcggt
     1741 aagacgtaga ggccagagtc gattttgcaa aatgtactct cccgagtggc taagaggatt
     1801 cgcgtaagtg gcggtgaaat ttcagtatgc ctgagcaaaa aaaggcgcgc aattaggcgt
     1861 cgccgaagta ttcaccggat cgcaagtgga cgccgttggc aaaatcgacg gcggagactt
     1921 gcttgcgccc ctccggtttg acgtgcgaga catacaaaaa ggattgctgg ccgcaggcaa
     1981 ccaggagttc tttaccgtcg taaaggattt ggccgggagc gccggcgctg aaagagtcag
     2041 acgccggttg gccgagaagg tggcaggtgg tggcgcggaa atgggtatag gcgccgggcc
     2101 aggcagtgaa gccgcgcatg cggttgtaga tgacttgagc gggctgctgc cagttgattt
     2161 gaccgctttc ttttttgagc agaggcgcga cggtggcggc ggagtgatcc tgcggctcca
     2221 gcttcaaagt gttgtcgcgg aggccgcgca gggtttcggc catgagaggg gcgcctagtt
     2281 cggagagccg ttcggcgagt tggggagtgg tctcggtcgg gccgatggga gtttcacgct
     2341 gcagaaggat gtcgccggtg tccatgccgg cgtctatgcg catggtagtg atgccggtgg
     2401 cggattcgcc gttggcgatg gcccactgga ttggagctgc tccgcgatac ttcggaagta
     2461 aagaagcgtg gaggttgatc cagccgaggc gcgggatgtt gagcagacgc tcgggaatga
     2521 tttggccgta ggcgatgatg acgatagcat cgggatcggc ttcacggaga aagtgctcgg
     2581 actcgagggt gcggattttt tcgggttgga agacggggat gtttgcggcg agcgcgactt
     2641 cttttaccgg tgaaaaagag aggtcatggc cgcggccacg ggggcggtcg ggttgggtga
     2701 tgacggcggc gatttggaaa tcggattggg cgaggaggtg cttgagcgta gggacggcga
     2761 agcttggagt gccgcagaag agaaggcgca tggttgggtt cgtgtggaat tatacgttga
     2821 gatccttcgt ccgcctgcag cagactcagg acgacaaacc tcaagagaaa gattcggagt
     2881 cacacagagg gcgcagaggt cgaacagaga gttcaccgag aaggctagag tcaccactcg
     2941 ccttgctttt ttagtttctt gattttgcgc ttgatgaggt cgcgcttgag catgctgagg
     3001 tgggccagga agagaatacc gttgaggtgg tcaatttcgt ggcagaaagc gcgcgccagc
     3061 aaatcttcgc cgcgaatctc gaagggttcg ccttttagat tttgcgcttt gatggtgacg
     3121 aattgcgggc gaacgacgta gccgcggaaa ccggggatag agaggcagcc ttcttcttcg
     3181 cgtttttcgc cttcgccgtg gatgacttcg ggattgacga gaacaatttt ggcttctgga
     3241 tttttgccgc tggtgacgtc cacgacggcg aggcgaatgc ttttgccgat ttgcggcgcg
     3301 gccaagccga cgccttgggc ggcgtacatg ctggcgaaca tgtcctcggc aagctcttcc
     3361 agttcggcgt cgaattgggc gatggtcttg gcgggctttt cgagaatcgg atcgccgtat
     3421 ttgacgatgg gatagatttt gcgcggcggt gcagagactt cgggcgcagc agcgttcgcg
     3481 gcggcgatgg tttcttcaga agtcgtggat ttgtctggca tagccctcga agttgcggcg
     3541 agtctccgtc aaggagtcac ccccaaattt ttgcagaaaa gcatcggcga ggacaagtgc
     3601 aaccatagct tcgccggcaa ccccgccggc cgggacgacg caccagtcgg agcgttcgta
     3661 agcggcctgg acgggctgtt tggtgaccat gtcggcggtt ttcaaagcgc gccgaagagt
     3721 ggagatgggc ttcaagtagc cgcgcacgac gacatcttca ccgttggtga tgccaccttc
     3781 gaggcctccg gcgcggttgg agctgcgaga gaatttgtgg gtggactcgt cgtaggagat
     3841 ttcgtcctgc acttcgctgc cgaaggagcc cgcagcgaga aggccggcgc cgatttcgac
     3901 gcctttgacc gcttgcacgc tcatgacagc ttgggcgagc cggccatcga gtttttcgtc
     3961 ccactgggcg tgcgagccga ggccgaccgg aacgttgtgg acaacaattt cgaagacgcc
     4021 gccgacggtg tcgcctgctt tgagaacgga atccacttcg gctttcatct tgtcctgcgc
     4081 ggcggaatcc acgcagcgga gcggtgattc gaggtcgttg gagatggcca gaatttcttc
     4141 ccaggagggc gagcggtcag cgcgagtgtg gccgacttga atagtgtggc tggcgatttc
     4201 gatagcgaag gcacgtagga gttgtttggc aagcgcgccg gcggcaacgc gggcagcggt
     4261 ttcacgggcg gaagcgcgtt cgaggatgta gcgagcatcg tggaagttga atttctgtga
     4321 accggcgagg tcggcgtggc cggggcgcgg agcgacgagt tttttggcgg tggagtcgtc
     4381 ttcgggatag gcctcgacgg cgagaatctt ttcccagttg gcccaatcgc ggttttcgat
     4441 gcggagagcg ataggcgcgc cgatggtctg gccgtggcgg actccggcga agatatcggc
     4501 gcgatctttt tcgattttct gtcggccgcc gcggccgtag ccgagctggc ggcggtggag
     4561 gtcgcgattg atgaattcga gatcaatggg cagacccgcg ggcaggccgg acagaaagga
     4621 gagcagcgcc tggccgtgcg attcacctgc ggtgtagaac ttcagcatgt gtctcgaatc
     4681 cctgaaatgg aaaagcgaaa tgcgaaaaac gaattttgga aaagtgaaga tagagtttaa
     4741 ctggcgcagc gagtcgcgtc taggcgctcg ctaagtcgtc taaggagact agatctcagc
     4801 aatccgaccc atggacgtag gcagttcctc acacgaggtg ttacgttcac cttccccggg
     4861 cgactggggt aaactagatc ggccgggcca aaggtccttt cttcttggag gctcctgtgg
     4921 cgcctacagc cagcacaccc ctcattcccg aaccagcccc gcaaaagaca gcgctggtag
     4981 ttgattccac cccagcggtc aacgcgatgc ttacccgggt cctcaaggac caggggatag
     5041 acatccgacg ggctgtggac aataagaccg tcctttcact tgtgaaggaa agtcccttcg
     5101 acctgatcat caccggccaa aagacctgcg ggagagaaga cgttgcactg ttgcacgcca
     5161 tccgaaacgt tcgcccgaat gtcaggatga ttattctcgc cgacgagagc acgcccgccg
     5221 atgtgatcga cgcggttcgg ggtggggcgt tcagttattt ttgtcctccc tacacgcgag
     5281 gtggattcgt cgagatggtg catctggcga tgacgcaacc tgcgtgggat gacgggatcg
     5341 agattgtttc cgcaaccaca tcctgggtcc gcctgatcgt gcgttgcgag gtggcgacgg
     5401 ctgaccgctt agtgcaattc cttcgtgcag ggtccacatt accggagggg gaaaaagaag
     5461 acgtgatatc ggcgttccac gaaatcctga tgaacgccat cgagcacggc gcgcacttcg
     5521 accccagcca gtacacagaa gttgcgttcg tgcgggctcg ccgggcgat
//
LOCUS       DASTPM010000442         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_390611, whole genome shotgun sequence.
ACCESSION   DASTPM010000442 DASTPM010000000
VERSION     DASTPM010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390611"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFR42_07810"
     CDS             <1..290
                     /locus_tag="VFR42_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369098.1"
                     /translation="SFGGLLVEHAAARGATALLRGIRAISDYEYEWQMALMNRRLQPG
                     LETVFMMASEAHSFISSRLVKEVFSLGGNVAGLVPPSVEARLRGRLTQATG"
     gene            442..1632
                     /locus_tag="VFR42_07815"
     CDS             442..1632
                     /locus_tag="VFR42_07815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0369099.1"
                     /translation="MPAVANAIAERISNISESSTMKVAADAAKLRADGVDVVDFGAGE
                     PDFPTPDNIKQAAIRALDQNFTKYTAAGGILDLKKAVCERHAQDFGTDYKPNECLITV
                     GGKHVIFNLTQALINPGDEVVIPVPYWVTYKDVVNFAGGKCVFVDTDESEGFTLTAAM
                     MEPHLTARTKLVLMNSPSNPSGAVMDRGEFEKIFRLCADRGIYLMTDECYCKFLYDSQ
                     PYSIASMPGAKETVLVAGSLSKTYAMTGWRIGFGLAPAEIINAMNKLQSHSTSNPTSI
                     AQKAAVEALRGPQDSVAAMLAEYHKRRDFVVKRLREIPGVECQEPRGAFYAYPNLKVV
                     IGKNGIRDSLALSERLLAEAHVAVVPGEAFGTDHHVRISYATSMHELSRGLERIHQFV
                     VKNS"
     gene            1635..>2009
                     /locus_tag="VFR42_07820"
     CDS             1635..>2009
                     /locus_tag="VFR42_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HEU0369100.1"
                     /translation="MPRPQRLTTSLRERVWGRRRLAPWFPDSDRPIGEAWYLSDRDLP
                     LLVKMLFTSERLSVQVHPDDGEDGARGKTEMWHIVDAEPGATIALGFRQPMTRDRLLA
                     STKTGEIEQSLNWVPVHAGETYF"
BASE COUNT          429 a          573 c          622 g          385 t
ORIGIN      
        1 attcgtttgg cgggctcctg gtggagcacg ccgcggcgcg cggggctacc gctctgttgc
       61 gcggcattcg ggccatttcg gattacgagt acgaatggca aatggcgctc atgaaccgga
      121 ggctccagcc ggggcttgaa acggttttca tgatggcgag cgaggcgcat tccttcatca
      181 gttcccggct ggtgaaggaa gttttcagtt taggcggaaa tgtcgccggg ttagtgccgc
      241 cgtcggtcga agcgaggctt cgagggcggt tgacgcaggc aactggataa aagccgcagg
      301 caactggata agaggccgca ggcaactgga taaaagtcgc aggtgactgg atagcaacct
      361 cgtaggcgac cggacaacaa gccgccgcca cggggtcacg gaaatcgcag ttaccgttta
      421 ataggaaggg tataagttac gatgccagca gtagccaacg caatcgcgga acgaatctcg
      481 aatatcagcg aatcctccac catgaaggtc gccgccgacg ccgcaaagct tcgcgccgac
      541 ggcgtggacg tggtggattt cggcgcgggc gagcccgatt ttccgacgcc ggacaacatc
      601 aagcaggctg ccatccgcgc ccttgaccag aacttcacca agtacaccgc ggccggcggc
      661 atcctcgatc tgaagaaggc ggtttgcgaa cgccacgcac aggatttcgg aacggattac
      721 aagcccaatg aatgcctgat caccgtcggc ggcaagcacg ttattttcaa cctgacgcag
      781 gcgctgatca accccggcga cgaagtagtg attccggtcc cgtattgggt gacatataag
      841 gacgtcgtga atttcgccgg cggcaaatgc gtctttgtag acaccgacga gagcgaaggc
      901 ttcacgctga cggctgcgat gatggagccg catcttaccg cgcgaaccaa gctggtcctc
      961 atgaattcgc cctcgaaccc gagcggcgcg gtgatggatc gcggcgaatt cgagaagatc
     1021 ttccgcttgt gcgcggatcg cggcatctat ctgatgaccg acgaatgcta ctgcaagttc
     1081 ctgtacgaca gccagccgta ctccatcgca tcgatgcccg gcgcgaagga aacggtgctc
     1141 gtggccgggt cgctttctaa aacgtatgcg atgaccggtt ggcgcatcgg cttcgggctg
     1201 gctcctgctg agatcatcaa cgcgatgaac aagctgcaga gccattccac ctcaaaccct
     1261 acgtcgatcg cgcaaaaagc cgcggtggag gcgctgcgcg ggccgcagga ttccgtagcc
     1321 gcaatgctcg ccgaatatca caagcggcgt gatttcgtgg tgaagcgact gcgcgagatc
     1381 cccggcgtcg aatgccagga accgcggggc gcgttttacg cctatccgaa tttgaaagtg
     1441 gtaatcggca aaaacggcat tcgcgattcg ctggccctct ctgaacggtt gctcgcggaa
     1501 gcgcacgtgg cggttgttcc gggagaagcc ttcgggaccg atcatcacgt tcgtatctcc
     1561 tacgccacat cgatgcatga gttgtcgcga ggcctggagc ggattcacca gttcgtggtt
     1621 aagaattcgt agtgatgccg cggcctcaac gcctgacgac ctcgcttcgc gagcgggtgt
     1681 ggggtcgaag acggcttgcg ccgtggttcc ccgattccga caggccaatc ggcgaagcgt
     1741 ggtatctctc ggatcgcgat ctgccgctgt tggtgaaaat gctgttcact tcggagcggc
     1801 tctcggtaca agtacatccc gacgatggag aggacggcgc gcgaggcaaa accgaaatgt
     1861 ggcacatcgt cgatgccgag cccggcgcga caatcgccct tggattccgt caacccatga
     1921 cgcgcgaccg gctgctcgct tccacaaaga ccggtgagat cgagcagtca ctcaattggg
     1981 ttccagtgca tgccggcgag acatatttc
//
LOCUS       DASTPM010000443         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_736511, whole genome shotgun sequence.
ACCESSION   DASTPM010000443 DASTPM010000000
VERSION     DASTPM010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736511"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /gene="chrA"
                     /locus_tag="VFR42_07825"
     CDS             complement(<1..962)
                     /gene="chrA"
                     /locus_tag="VFR42_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEU0369101.1"
                     /translation="MAEIARAFLRLGFVAFGGAAAHIGLMEEEFVRRRKWLSREEFLD
                     RLGAVNLLPGPTSTEMAIYLGELRGGFAGLLVAGACFILPAAFMTLGLAWAYVRYGAL
                     PELAGVLWGVKPVVVVLLVQAVRALGKAALKSRELMFLAAVVLGLAVVHVAPLALLIG
                     TGVAWIVATRFAEKRSGAAAGSTASMIIGDAASNRVAAIGGVGSFVAVTTSGVFLYFL
                     KIGALLFGSGYVLLAVLREDLVQRLHWLTEPQLLDAIAVSQATPGPFFTVATFVGYLL
                     AGWHGAGVATLGMFVPAFTYVALTAKVLPRMRKSPNAGAFLDGV"
     gene            1055..2686
                     /locus_tag="VFR42_07830"
     CDS             1055..2686
                     /locus_tag="VFR42_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HEU0369102.1"
                     /translation="MCTHMNLCAMLWALAQNPVSNYWHKLTDKTFTGIYHVNAFDLMM
                     MIPYFVVLIILAVYGLHRYWLVYDYYKYRKNVPGPPPPVVQWPRVTVQLPIFNERYVI
                     ERLVEAIAKFDYPPELLDVQVLDDSTDETQQVARACVERHAAQGAPISYIHRTNRQGF
                     KAGALENGLRTARGEFVAIFDADFIPESDFLRRTIPYFQKPEIGMVQTRWTYLNRDYS
                     LLTQVETILLDGHFVVEHGARSRRGTFFNFNGTAGVWRRSAIDAAGGWQHDTLTEDTD
                     LSYRAQLKGWKFLYLPDIECASELPVDMNGFKAQQARWAKGLMQTAKKILPQVFQSKA
                     PWHVKAEAFFHLTANISYPLMVLFSAMLLPAMIVRFYQGWLQMILIDLPLFLASTCSI
                     SSFYLVAQKELRPKSWWRTFLYMPFVMATGIGISVRNAKAVLEALFGVQSEFARTPKF
                     KIEGKKDTFVAKKYKNKAGWMPYAEISLGIYFAFTVVYAVSNQNYATIPFLLLFVWGY
                     LYTGCMSLGQTYFAHLRFGVNAPEMRPAASGAPGF"
BASE COUNT          602 a          921 c          689 g          572 t
ORIGIN      
        1 ttcacgccgt ccagaaaagc ccccgcgttt ggcgacttgc gcatgcgcgg caacaccttc
       61 gctgtcagcg ccacataggt aaacgccggc acaaacatcc cgagcgtcgc cactccggcg
      121 ccgtgccacc cggccagcag gtaacccaca aacgtggcca cggtaaaaaa agggccgggc
      181 gtcgcctggc tcacagcgat cgcatcgagc aactggggct cggtcaacca gtgcaggcgc
      241 tgcaccaaat cttcgcgcaa taccgccagc agcacatatc cgctgccaaa caacaacgcg
      301 ccaatcttca ggaaatagag gaacactccc gacgtagtca ccgcgacgaa cgaacccact
      361 cccccgatcg cagcaaccct attagacgca gcgtcgccga taatcatgct cgccgtcgag
      421 cccgcagccg ctccactgcg cttctcggca aagcgtgtcg ctactatcca cgccacgccg
      481 gttccgatca gcagggccag cggagccacg tgaaccaccg ctaatcccag caccactgcg
      541 gccaaaaaca tcagctcgcg cgatttcagc gccgccttgc ccagcgcccg caccgcttgc
      601 accagcagta ccaccacaac cggcttcacg ccccacagaa cgccggccag ttccggcagg
      661 gccccgtaac gcacgtaagc ccatgccaat ccgagcgtca tgaaagcggc cggcaaaatg
      721 aaacatgctc ccgctaccaa caacccggcg aagccgccgc gcagctcgcc taggtagatg
      781 gccatctccg tcgacgttgg ccccggcaac aagttcaccg cgcccagccg gtctaggaat
      841 tcctcgcgcg agagccattt gcgccgccgc acaaattcct cttccattaa gccgatgtgc
      901 gctgcagctc cgccaaacgc cacaaatccc agacgcaaga acgcccgcgc aatttcagcc
      961 aagcgaccgg ttctcgtcga ttcttcctgc attgccttta ccgcttctgg caagcctgcc
     1021 gctcctctgg cgcaccgggt ctaaagcccg taaaatgtgc acgcacatga atctctgcgc
     1081 catgctctgg gccttggccc agaatccggt ttccaattac tggcacaagc tgaccgataa
     1141 aacgttcacg ggcatctacc acgtcaacgc cttcgacttg atgatgatga ttccgtattt
     1201 cgtcgtgctc attatccttg cagtttacgg gttgcaccgt tactggctgg tttacgacta
     1261 ctacaaatat cgcaagaacg ttcccggccc tccgcctccc gtcgtgcaat ggcctcgcgt
     1321 aaccgtccag ctcccgattt ttaacgaacg ctacgtgatc gagcgcctcg tcgaagcaat
     1381 cgctaagttt gactatccgc ccgaattgct cgacgtccaa gtgctcgacg attctaccga
     1441 tgaaacccag caagtggcgc gcgcttgtgt ggaacgccac gccgcgcaag gcgcgccgat
     1501 cagctacatc catcgcacca atcgccaggg tttcaaagct ggcgcgctgg agaacggcct
     1561 aaggacagct cgcggcgaat tcgttgccat cttcgacgcg gattttatcc cggaaagcga
     1621 ttttctccgg cgcaccatac cttattttca aaagcccgaa atcggcatgg tgcagactcg
     1681 ctggacttat ttgaaccgcg actactcgct gctcacacag gtggaaacga tcttgctcga
     1741 cggccatttc gtggtcgaac acggcgcgcg ctcccgccgc ggcacgttct tcaacttcaa
     1801 cggcaccgcc ggcgtttggc gccgttctgc aatcgacgcg gccggcggct ggcagcacga
     1861 tacgctcacc gaagacaccg atctgtccta tcgcgcacaa ctcaaagggt ggaagttttt
     1921 gtatttgcca gatattgaat gtgccagcga attgccggta gacatgaatg gtttcaaggc
     1981 gcagcaggcg cgctgggcca aggggctgat gcagaccgct aagaaaattc tgcctcaggt
     2041 gtttcagtcc aaagcgccct ggcacgtcaa agccgaagca ttctttcacc tgaccgcgaa
     2101 catcagctat ccgctcatgg tgctgttctc ggccatgctg ctaccggcca tgatcgtccg
     2161 cttctatcag ggctggctgc agatgattct gatcgaccta ccgttgttcc tcgcgtcgac
     2221 ctgttccatc tccagtttct atctcgtagc gcaaaaagaa ttgcgcccca aaagctggtg
     2281 gcgcacattt ctctatatgc cgtttgtcat ggccaccggc atcggaatct ccgttcgcaa
     2341 cgccaaagcc gtgttagaag cccttttcgg cgtgcaaagc gagttcgcgc gcacaccaaa
     2401 attcaagatc gagggcaaga aagacacttt cgtcgccaag aaatacaaaa acaaagctgg
     2461 ctggatgccc tatgccgaaa tttcgctcgg catctacttc gcgtttaccg ttgtgtacgc
     2521 cgtctctaac cagaactatg cgacgattcc ttttctgctt cttttcgtat ggggctatct
     2581 ctataccggt tgcatgtcgc ttggacaaac ttacttcgcg cacctgcgct tcggcgtgaa
     2641 cgcaccggaa atgcgcccag ccgcttctgg cgcccccggg ttttagctgc ggctgaaacc
     2701 gtgaatcgtc ttcgttttcg ctgcaaggag tgacaccgtg aaaattggcc ttgcctctct
     2761 ggctgctgtg ctcttattgc tctt
//
LOCUS       DASTPM010000444         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_246159, whole genome shotgun sequence.
ACCESSION   DASTPM010000444 DASTPM010000000
VERSION     DASTPM010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246159"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFR42_07835"
     CDS             <1..297
                     /locus_tag="VFR42_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369103.1"
                     /translation="QKEDALKYDEPPGWLIPVRHSLGAVLMKQQRFAEAEQVYRDDLV
                     RLPENGWSLLGLAESLRKQKKNADEVAQTQAKFKKVWAKADLTITTSCLCQPQA"
     gene            complement(331..1107)
                     /locus_tag="VFR42_07840"
     CDS             complement(331..1107)
                     /locus_tag="VFR42_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369104.1"
                     /translation="MPLMTFSAHARAQALAPASLSGESRTEETTSDVVQVITAKTAPA
                     ISKRTRKLMAESARTSAEKPVPVVAQKPTQPVVKPTARATFTMEPVRPMNQPTVNQTV
                     QHGSRFSFRSPQGKKESVPVISDYYPKRIVYPYAKIDRRIDNRLVQAATLAQERAHAH
                     SRSRCWHYVKDALLASGVIDSRPKTDLARDAASELVNNYGFKKLSITDPFAAPVGSVL
                     VYGTARSVGHVELRTKDGFVSDFRSPTPSKRPLMGVYAKL"
     gene            complement(1271..>3004)
                     /locus_tag="VFR42_07845"
     CDS             complement(1271..>3004)
                     /locus_tag="VFR42_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369105.1"
                     /translation="NGTARSWLNRGQRGLVMERTTFLKNYRIRLKYDGSPYEPDRNGP
                     AISHEAVDERTAEPVSVTLIPAKSIDPAERQEFEEHVSAAQKLRHANIAKVLDFGREG
                     DDYVYVSERLAGETLASWVRSHGPMAADAALRVGEQIVSVLSSADFHKLPYPSIRPSD
                     IMLVPGQTAEGSWPLVKVTNFGLPALMARPEPQLTEEEMPSQAPFGGQVTSDQQFGLP
                     TKDIRSEIYSLGVTLYFLLTGVALSAETLQRGPKFSGFPKPLRVLLGQLLHRDPDQRP
                     KDLLVVAEMMRASLGKIERRRAFSDRYGIPLRTSVARRAEARPRRLVRTAVVVAALLV
                     LAAAIAPVVFPDSIGKLVLGRQKQVGVLIGVPDSSPAASQPPALAQVPSAGPSAPPGI
                     VSSQPPNPAAVPETPAPTNAAATPNPFHVAPADVQQAQVASAQLQGAAPANSSENSAA
                     PTPDISGSVAPDTNSSAQANTEPPPTTASQSSSQGTEKSVASKPKRTRAGRSSAAYSQ
                     QGRTRSMRSRVTGITSDGRLILRLPSGRTAIVAPDEDESVPRHRNRVYIDRDQVLGPP
                     PGFEPDYFPHD"
BASE COUNT          615 a          850 c          887 g          652 t
ORIGIN      
        1 caaaaggaag acgctctcaa atatgatgag ccacccggat ggttaattcc ggtgcgtcac
       61 tcgctcggag cggtgttgat gaagcaacaa cgttttgccg aagccgagca ggtctatcgc
      121 gacgatctcg ttcgtttgcc cgaaaacggc tggtctttac ttggcttggc agaaagtttg
      181 cggaaacaaa agaaaaacgc agatgaagtt gctcaaacgc aggcgaagtt taaaaaggtt
      241 tgggcaaaag ccgatctgac cattaccact tcgtgccttt gccagccgca ggcatgagct
      301 gcgcggatgt tctctccaag cggagacgaa ttacagcttt gcgtaaacgc ccatcagcgg
      361 gcgtttcgat ggtgtcggtg aacggaagtc gctgacgaaa ccgtcctttg tgcgaagctc
      421 aacatgacca acgcttcgcg cggtaccata cacgagcacc gaaccaacgg gcgcagcgaa
      481 tggatccgtt attgatagtt ttttaaatcc gtagttgttg acgagttcgg acgcagcgtc
      541 tctggcaagg tcggtttttg ggcgggagtc gatgactcct gaagcgagca acgcgtcttt
      601 gacatagtgc cagcagcgcg accgtgaatg cgcatgcgcg cgctcctgcg cgagcgttgc
      661 tgcttgcact aatctattgt cgatgcgccg atcaattttg gcataggggt aaacgattcg
      721 cttcggataa tagtcgctaa tcacgggcac ggattccttt ttaccctgcg gagatctgaa
      781 actgaaacgg ctgccgtgct gcaccgtttg atttacagtc ggctgattca tcgggcgcac
      841 cggctccatc gtaaaggttg cgcgggcggt cggtttcacg actggttgcg tcggcttttg
      901 ggcaaccacc ggcaccggtt tctcagcgct tgtacgtgcc gattctgcca tgagttttct
      961 tgttcttttc gagatcgccg gcgctgtctt cgccgtaatc acctggacca catcgcttgt
     1021 ggtctcctca gtgcgacttt cgccacttag cgaagcaggc gccagcgcct gcgcgcgggc
     1081 gtgcgcgctg aaagtcatca aaggcaacgc agtcgccagt gctaagaaga ttggacgtcc
     1141 ccgcacgagc gcgaggtatg ccggaaatca caaaccatgg caagcttttt tttcgataaa
     1201 aaccgttggc ggctggtcgg ttacctaggt cacgggtttc cacgcacacg gaatggcgcc
     1261 ccgcctcgtt tcaatcgtgt gggaaataat cgggttcaaa cccgggcgga ggaccgagca
     1321 cctggtcacg gtcgatgtaa acccggttgc gatgacgcgg caccgattca tcttcgtccg
     1381 gcgccacgat cgcggtgcgc ccagacggca gacgcaggat caatcgtcca tcggatgtga
     1441 tacccgtgac ccgactgcgc attgaacgag tgcggccctg ctgggagtat gcggcagaac
     1501 ttcggccggc acgtgtgcgt ttgggtttcg aggcaacaga tttctcagtt ccctgcgaac
     1561 tcgactggct ggcggtagtt ggtggcggtt cggtatttgc ctgagccgat gagtttgtgt
     1621 ccggggctac agaaccagag atgtcgggcg tcggtgccgc agaattctcc gatgaattcg
     1681 caggagcggc cccctgcagt tgagcgctcg ctacttgagc ctgctgaaca tcggcgggtg
     1741 cgacgtgaaa cggattcgga gtcgccgccg cgtttgtcgg cgccggagtt tcaggtacgg
     1801 cagctgggtt cggcggttgc gatgacacaa tgccaggcgg cgcggacgga ccggcgctag
     1861 ggacttgagc cagagcgggt ggctggcttg ccgctggtga agagtccggc acaccgatca
     1921 gaacgccaac ttgcttttgc ctcccaagaa ccaacttgcc gattgagtcc ggaaaaacca
     1981 cgggcgcaat tgccgccgcc aacaccagca acgcggcgac cacgaccgcg gttcgtacaa
     2041 ggcgacgcgg ccgagcttct gcgcggcggg cgacgcttgt ccttagcggg atcccgtatc
     2101 gatcggaaaa tgcgcgccga cgctcaattt tccccagcga cgcgcgcatc atctccgcca
     2161 cgacgagcaa gtcctttgga cgctgatccg gatcgcgatg caacagctgg cctaacaaca
     2221 ctcgcaatgg ctttggaaat ccggaaaact tcggaccacg ttggagcgtc tcagccgata
     2281 gcgcgacgcc ggtaagcaaa aaataaagtg tcacacccag tgaatagatt tccgagcgaa
     2341 tgtcttttgt tggcaggccg aattgttggt cactcgttac ctgtcccccg aagggagctt
     2401 ggctaggcat ctcctcttcc gttaactggg gctccggccg agccatgagc gccggcaagc
     2461 cgaagttggt caccttcacc aatggccaac tgccttctgc cgtctgccct ggcaccagca
     2521 ttatgtcgga aggccgaatc gacggatacg gcagcttgtg aaagtccgcc gaggataaga
     2581 cacttacgat ctgctcgccg actcgcaatg cggcgtcagc tgccatgggc ccgtgactgc
     2641 gcacccaaga agccagtgtt tcgcctgcga ggcgctcgga aacgtagacg taatcgtcgc
     2701 cttcgcgtcc aaaatcgagg accttggcga tgttggcgtg ccgcagcttc tgcgccgccg
     2761 acacatgctc ctcaaattcc tgccgttcgg ccggatcgat actctttgcc gggatcagcg
     2821 ttacagaaac gggctccgcg gtcctctcat ctacggcttc gtggctgatt gccggaccgt
     2881 ttcggtcggg ttcatacggg ctgccatcat atttaagacg gatgcgataa ttttttagga
     2941 atgtcgtgcg ctccataaca aggcctcgtt gcccacggtt caaccacgat ctggctgtac
     3001 cgtt
//
LOCUS       DASTPM010000445         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_102164, whole genome shotgun sequence.
ACCESSION   DASTPM010000445 DASTPM010000000
VERSION     DASTPM010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102164"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..2357
                     /locus_tag="VFR42_07850"
     CDS             78..2357
                     /locus_tag="VFR42_07850"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0369106.1"
                     /translation="MAFAQRERGELRIEVKDPQGRPAPAAAQLISESNQLKHEFPIAA
                     EGKYIAFELPFGVYRLTIAAEGFADWTDLVEIRSEVPVRVAVTLGVASVNTRVEVTDA
                     ATLVDPSETGTIYSLSGERLREHGSSQTGRSLSDAVNDQPGWLYEANGILHPRGSEYQ
                     VQYVLDGMPLTQNRSPAFAPSFDAGDIESLRVLTAGYAAEYGRKLGGIVELTTEKNPP
                     AGLHGRVELGGGSFASIGGAAELGYSAGANHFEASVQGFHTDRYLDPPVLDNFTNRAN
                     GSGFSAAYEREFSERDKLRFTVTRSETRYIVPNERVQQVAGQRQDAASIETSGQIYFQ
                     HIASPNLLWSLSASVRDDSFTLQSNDLSTPVIVSQDRGYREGYSRADISWHRGHNDWK
                     AGIDVLFTPVHESLSYQITDPSQFDPGTLAQFTFNERKWDVEPAFYVQDTFHAGNWNV
                     SAGLRFDHYGFVAHQSAWSPRLGVSRYIPLSKTLLHFSYDRVFQTPAMENLLLASSPE
                     VDSLNPLVVRLPVRPSRANYFEGGLTQALFGELRLDANVFRRNFRNFADDDVLLDTGI
                     SFPIAFDNARIIGEEIRLAVAEWGRFSGFLSYSNQSASAQGPITGGLFLGSEAGDELT
                     DTTRFAVTQDQRNTVRARVRAQVSKRVWAAVSANYGSGLPVELDTADIDLNFLLTQYG
                     EEILSRVNFDRGRVKPNFSLGAAAGAELYRKETRTLNLQVEAANLTDRVNVLNFAGLF
                     SGTAVAAPRSASARLRFTF"
     gene            complement(2446..3201)
                     /locus_tag="VFR42_07855"
     CDS             complement(2446..3201)
                     /locus_tag="VFR42_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU0369107.1"
                     /translation="MAGFFGNSNPVLKEKTFEGQLPHIAAGEAMTVQGTVNKTGILLL
                     CVVATSAWTWGLAHSEQPEAAYPWMIGGLIGGLVTCLVTVFKKEWSALSAPIYALFEG
                     LVLGGLSAIFEQRYPGIAVQSVALTFGVLFVLLLAYKTGLIRATRGFTLGIVCATGAI
                     ALMYTVNMLMRVFGSSGMSILYASTPLGIGISVVVVIIAALNLILDFDLIERGAAMGA
                     PKYMEWYGAFALMVTLIWLYMEILRLLGKVRRR"
     gene            3701..4885
                     /locus_tag="VFR42_07860"
     CDS             3701..4885
                     /locus_tag="VFR42_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0369108.1"
                     /translation="MGQHEVSEGWIGKVKQSKDKESTNYFMWLTIDGQKQRRATGTDD
                     PDKAVDMLAEWRSQAKVGFRKDARLRYEEIRDDYLKNGGKIQESVRRDLDVYFKNIRI
                     GAIGDKLNAFREWRENLDRVVEYKQETLAKEIALRKMKAMKDRRKPLSAVEIAKIENE
                     ATEWVEKGVQTTTDKRLVYLRAMFYHAFEKTKKINLGDIPYFPIRGKAADNVRQGKFS
                     EADFSNILAKLPEYLHPLVKFLHLTGMRSGQARAITWDMIDEDNVLRMPGFVTKNGQP
                     YSLALTNRKGEPYEATAFLVNMKTRPHGESVFDTRNLREEWRMACHKLKLGMFDPKTR
                     SYRGAQLHDFRRTAASNMNAKGIQEGKAMTVTGHKTNSMYKRYGIEELDSQRDALDAV
                     TV"
     gene            4914..4990
                     /locus_tag="VFR42_07865"
     tRNA            4914..4990
                     /locus_tag="VFR42_07865"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4948..4950,aa:Glu,seq:ttc)
     gene            4997..>5301
                     /locus_tag="VFR42_07870"
     CDS             4997..>5301
                     /locus_tag="VFR42_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369109.1"
                     /translation="MVTRTPGGNAQLVKVQYGLLKAAVNDYFDGVEARALDVAVRIRT
                     LVHNTGNSHPFVATIDPTVYEVHNNGAWYNFGYLFGLACFFGGGGKSSARRKKISTA"
BASE COUNT         1211 a         1703 c         1352 g         1035 t
ORIGIN      
        1 gtaagccagc ttcagctagc caccgaattc tgcgcttagc ctcagcgagc ctactggtct
       61 ttctggcctg cccatacgtg gcctttgccc aacgcgagcg cggcgaactt cgcatcgaag
      121 tcaaagaccc gcaaggccgc cccgcccctg ccgccgcgca gctcatcagc gaatccaacc
      181 agctcaaaca tgaattcccc atcgccgctg aaggtaaata catcgccttc gaacttccct
      241 tcggcgtgta ccgcctcacc atcgccgccg aaggcttcgc cgattggacc gacctggtcg
      301 aaattcgcag cgaagttccc gtgcgcgtcg ccgtcacgct cggcgtcgcc tccgtcaaca
      361 cccgcgtcga agtcaccgac gccgccacgc tggtcgatcc ttccgaaacg ggcaccatct
      421 actccctcag tggcgaaaga ctccgcgaac acggctcctc ccagaccggt cgttctcttt
      481 ctgacgctgt aaacgaccag cccggctggc tgtacgaagc caacggcatt ctccacccgc
      541 gcggctccga atatcaggtg caatacgttc tcgacggcat gccgctcacg caaaaccgct
      601 cgcccgcgtt tgctccgtcc ttcgacgccg gcgatatcga atccctacgc gtgctcactg
      661 ccggatacgc cgccgaatat ggccgcaagc tcggcggcat cgtcgaactc accaccgaaa
      721 aaaatccgcc cgcaggtttg cacggtcgcg tcgaactcgg cggcggcagc ttcgcctcca
      781 tcggtggcgc cgccgaactc ggctactccg ccggcgcaaa ccatttcgaa gccagcgttc
      841 agggattcca taccgatcgc tatctcgatc cgccggtgct cgacaatttc accaaccgcg
      901 ccaacggctc gggcttctcc gctgcgtacg aacgtgaatt ttcagagcgc gacaaactcc
      961 gcttcaccgt cacccgcagc gaaactcgct acatcgtccc caacgaacgt gtgcagcaag
     1021 tcgccggcca gcgccaggac gccgccagca tcgaaaccag cggccagatc tactttcagc
     1081 acatcgcctc gcccaatctg ctttggagcc tttccgccag cgtccgcgac gattccttca
     1141 cgctccaatc caacgatctc tcgacgccgg tcatcgtttc ccaggatcgc ggttatcgcg
     1201 agggctattc gcgcgccgac atttcctggc atcgcggcca caatgactgg aaagcaggca
     1261 tcgatgtcct cttcacgccc gtccacgaat cgctttccta tcaaatcacc gatccctcgc
     1321 agttcgaccc cggcacgctc gcgcaattta ccttcaacga gcgcaaatgg gatgtcgaac
     1381 ccgctttcta tgttcaggac accttccatg ccggcaactg gaatgtcagt gccggcctgc
     1441 gctttgacca ttacggtttc gtcgctcacc aatccgcctg gagcccccgc ctgggcgtct
     1501 cgcggtacat tccgctatcc aaaacgctgc tccacttttc ctacgaccgg gtgttccaga
     1561 ctccggccat ggagaatctc ctcctcgcca gttctcccga agtcgactcg ctcaatcccc
     1621 tcgtcgtccg ccttccggtt cgtccgtccc gcgcaaatta ctttgaaggc ggtctcaccc
     1681 aggccctttt cggcgaactc cgtcttgacg ccaatgtatt tcgccgcaac ttccgaaact
     1741 ttgccgacga tgacgttttg ctggacaccg gcatcagctt tcccatcgcc ttcgacaacg
     1801 cccgcatcat cggcgaagag attcgcctcg ccgtcgcgga gtgggggcgc ttttccggtt
     1861 tcctcagtta ttcgaaccag tccgctagcg cgcagggtcc catcaccggc gggctctttc
     1921 tcggcagcga ggctggtgac gaactcaccg acaccactcg cttcgcggtc acccaggacc
     1981 agcgcaacac cgtccgagcc cgcgttcgcg cgcaagtcag caaacgcgta tgggccgcgg
     2041 tcagcgcaaa ctacggcagc ggcctgcctg tcgaactcga caccgctgat attgatctga
     2101 atttcttgct gactcagtac ggtgaggaaa ttctttcccg cgtcaatttc gatcgcggcc
     2161 gagtcaagcc caacttctca ctgggagccg ccgccggggc ggaactctat cgcaaggaaa
     2221 ctcgcactct caatcttcag gtggaagccg ccaatctcac cgatcgcgtc aacgttctga
     2281 acttcgcggg gctcttttcc ggcaccgccg tcgccgctcc ccgcagcgcc tccgcccgcc
     2341 ttcgctttac cttttaaaaa tttgcgcctt agatttcgta gggcgcagcc cagtcctgag
     2401 gaatgccgcg ctccgtctgg ccacgatttc aactgcaaaa agcgactacc gccgccgcac
     2461 tttaccgagc agccgcagaa tctccatgta caaccagatc aacgtgacca tcaaagcgaa
     2521 cgcgccatac cattccatgt actttggcgc tcccatggcc gcgccacgct cgatcaggtc
     2581 gaaatccaga atcaggttca gcgccgcaat gatcacaacc accacactga tgccgattcc
     2641 cagcggcgtg ctggcataga gaatcgacat gcccgacgac ccaaacacac gcatcagcat
     2701 gttcaccgtg tacatcaacg cgatcgcgcc ggtcgcgcaa acgattccca gcgtgaagcc
     2761 ccgcgtagcg cgaatcaatc cagtcttgta cgccagcagc agcacaaaca gcacgccaaa
     2821 cgtcagcgcc accgattgca ccgcgatccc cggatacctc tgctcaaaaa ttgccgagag
     2881 cccgcccagc accagtcctt caaacagcgc gtaaatcgga gcactcagcg ccgaccactc
     2941 cttcttgaag acggtcacca ggcaggtaac caacccgccg atcaggccgc caatcatcca
     3001 cggatacgcc gcttccggct gttccgaatg tgccagtccc cacgtccacg ccgaggtcgc
     3061 caccacacac aagagcaaaa tccccgtctt gttgacggtt ccctgcaccg tcatcgcttc
     3121 gcccgctgcg atgtggggca attgcccttc gaatgtcttt tctttcaaca ccggattcga
     3181 gttgccaaaa aatccagcca taatggaacc tcccggtcgc cttactgccg actacgacgc
     3241 tattgtctca accgcgctac tgcgctttct tctgcttaaa gctcttgcag gaacattcgt
     3301 tcgtataggg cggcgtggat ccgtgctggc aaccgtgcga agcaccagcc tgtacatgct
     3361 cggagaacgc atgcccgcaa gtgcactggt cgttctgctt tgggggaggt ggcggagtgt
     3421 atttcatagt gtcccgtccg gagaaaccgg aaatcaagaa tgtatcgtag ccccttgcca
     3481 ggcagcaatc ctcgcccgca ctcagcgtaa tccacatgga aaggattttg gtaccgctaa
     3541 cgggaatcga acccgtattt tagccttgaa agggcggaat ccgcaactaa aaacccgcac
     3601 aaacaaaggc catttgcaaa tcaggcttaa cttttgcacc caaattgcac catactagcc
     3661 tctactatta taacaggcgg taaacatagg agagtgacct atggggcagc atgaagtcag
     3721 cgaaggctgg atcggcaagg tcaagcaatc caaggacaaa gagtccacca actacttcat
     3781 gtggcttacg atcgacggcc aaaagcagcg cagggccacg ggaacggacg atccggataa
     3841 agccgtagat atgctggcag agtggcgttc tcaggcaaag gtggggtttc ggaaggacgc
     3901 gcggctgcgg tacgaagaga ttcgggacga ctatctaaaa aacgggggaa agattcagga
     3961 aagcgttcgc cgtgacctgg atgtgtattt caagaacatc cgtattgggg caatcggcga
     4021 caagctgaat gcgttccggg aatggcgcga gaacctggac cgcgttgtgg agtacaagca
     4081 agaaacgcta gcgaaggaaa tcgctctgcg gaaaatgaag gcgatgaagg acagaaggaa
     4141 gcctctgtcg gccgtcgaga ttgccaagat tgagaacgaa gcgactgagt gggtcgaaaa
     4201 aggcgttcag acgacgacag ataagcgtct ggtctacctt cgcgccatgt tctaccacgc
     4261 cttcgagaag acaaagaaaa tcaacctcgg cgacattccg tactttccga ttcgcggcaa
     4321 agcggctgac aacgtcaggc agggcaaatt ttcggaagct gactttagca acatcctggc
     4381 gaagttgcca gaatacctcc acccgctggt gaagttcctg cacctgacag gaatgcgatc
     4441 aggtcaggcc cgagcgatta cgtgggacat gattgacgag gacaatgtcc tccgaatgcc
     4501 aggattcgtg acgaagaacg ggcagcctta ctccctggca ctgacgaaca ggaaaggcga
     4561 accttatgag gcgaccgcgt tcctggtcaa catgaaaact cgcccgcacg gtgagtctgt
     4621 gtttgacacg aggaatcttc gagaagaatg gcgaatggcc tgccacaaac tgaagctagg
     4681 gatgtttgat cccaagaccc ggagctatcg aggagcccag cttcacgact tccgccgcac
     4741 cgctgcaagc aacatgaacg ctaaaggtat ccaagaagga aaagcgatga ccgtcacagg
     4801 tcacaagaca aattcgatgt acaaacgcta cggcatcgaa gagttggatt cgcagcgaga
     4861 cgcgctcgac gctgtgaccg tgtgatagaa taatgggatt gactgttagc tgcggcccgg
     4921 tagtgtaatc ggttaacacg ttgccctttc aaggcaaaga ctacgggttc gattcccgtc
     4981 cgggctacca atttagatgg tgacgcgcac tcctggcggc aacgcgcaac tcgtcaaagt
     5041 acaatacggt ctcctaaaag ccgcagttaa tgattatttt gatggcgttg aggcacgcgc
     5101 actcgacgta gccgtgcgaa tcagaaccct cgttcacaac acgggcaaca gtcacccgtt
     5161 cgttgccacg atcgatccga ccgtctacga ggtccacaac aacggagcct ggtacaactt
     5221 cggctaccta ttcggcctag cctgcttttt cggtggcggc ggtaaaagct cggcgcgtcg
     5281 aaagaaaatc tcgacggcaa a
//
LOCUS       DASTPM010000446         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_563875, whole genome shotgun sequence.
ACCESSION   DASTPM010000446 DASTPM010000000
VERSION     DASTPM010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563875"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFR42_07875"
     CDS             <1..495
                     /locus_tag="VFR42_07875"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0369110.1"
                     /translation="ALTGLRGAGKSTLGSLLAQRLQCPFIELDRQIERDTGLTLAIIF
                     DLYGQAGYRRLERVALDRILERHRRFVLATGGGIVSDPGNYNRLLSTCLTVWLRAKPE
                     DHMARVIKQGDRRPMAQSRQAMRDLRRILYSREALYRQADAILDTSAQPLKHSVHSLL
                     RLIS"
     gene            complement(664..945)
                     /gene="pqqD"
                     /locus_tag="VFR42_07880"
     CDS             complement(664..945)
                     /gene="pqqD"
                     /locus_tag="VFR42_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEU0369111.1"
                     /translation="MSVDPNAKPQLAPGVRLGENQGQRVLLMPERALRLNGPSLEIVS
                     RCDGRHTVAQLVTELQKLYSKADPHKVESDVLGYLQLLHTQRALDFLPA"
     gene            complement(1012..1431)
                     /locus_tag="VFR42_07885"
     CDS             complement(1012..1431)
                     /locus_tag="VFR42_07885"
                     /inference="COORDINATES: protein motif:HMM:NF042613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6796 family protein"
                     /protein_id="HEU0369112.1"
                     /translation="MAFTILMVFGSAIHTLWTAKGLALKSCYGNDDASCRALLQAVNS
                     YWNIAYNIGAVPGYVGAILLIGLVLFGKSNYPRWTALANPAVLALLSPLADRTPAPFG
                     AILAGGFTNLSIALFFLVSILTTWDGSENRDRAPVAV"
     gene            complement(1714..2481)
                     /gene="pqqC"
                     /locus_tag="VFR42_07890"
     CDS             complement(1714..2481)
                     /gene="pqqC"
                     /locus_tag="VFR42_07890"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEU0369113.1"
                     /translation="MNYPQEKHPLSEEEFIAGFHAIGEERYHHKHPFHKLMHQGALTR
                     GQLQAWALNRYYYQSNIPRKDAALLARTDDHTFRTVWRKRLVDHDGDAADSGPDGPLP
                     GGIEKWLKLVEATGISRVSAVHGDGILPATRYAVDAYIHFVSTRSHLEAVASSLTELF
                     SKRLIALRMEKLREFYPWLSSGLDYFTGRLTQAPEDAEFALSWVVKHARTREEQDAAH
                     AALRAKCDILWAMLDALYYAYVQPGWPPPGAFHPDKQ"
     gene            complement(2478..>3233)
                     /locus_tag="VFR42_07895"
     CDS             complement(2478..>3233)
                     /locus_tag="VFR42_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0369114.1"
                     /translation="RTQILAAPELAPPDGEPTGTPIGGVFLPSADVDSLMGLLHLREF
                     QSFFLFATPAIQKIVQTENRIFDVLHRADPPVQWQSLTPGSRLACRFGEDPSAPPTFI
                     CSPISVGGGFPDYMSSELRRSLSSQESVIALRFEQSGKSFFFAPSLPEISGEWLKAAA
                     TSTVAFLDGTFWSDDELLSTGRGQKNAREMGHLPLSGRDGLLAQFPSDSAARKILIHI
                     NNTNPILDENSAEHRAVRDAGFEIASDGMEISL"
BASE COUNT          674 a          848 c         1107 g          604 t
ORIGIN      
        1 gcgctcaccg gcttgcgcgg agccggaaag tccacgctgg gttccttgct cgcccagcgc
       61 ctgcaatgtc cgttcatcga actcgatcgc cagatcgagc gcgacaccgg cctcacgcta
      121 gccattatct tcgatctcta cggccaggcc ggctatcgcc gcctggaacg cgtcgctctc
      181 gaccgcatcc tcgagcgcca tcgccgcttt gttctcgcca ccggcggcgg catcgtctcc
      241 gatcccggca actacaatcg actgcttagt acctgcctca cggtttggct ccgcgccaaa
      301 cccgaagacc acatggcccg cgtcatcaaa caaggtgacc gccgtcccat ggcgcaaagc
      361 cgccaggcca tgcgcgatct tcgccgcatt ctttacagcc gcgaggccct ctaccgtcaa
      421 gccgacgcca tcctcgacac ctccgcccaa cccctgaaac attccgtcca ctccctcctc
      481 cgcctaatct cctagtccac gctgcgtcct ccgcccggat caccattctc ccgaatggcg
      541 ttttaatccg gtccctggcc ttttgctgtt ccgtaatgca cacgctgttc gccgtagcac
      601 cgcgcaaatt agggttgccg ggggcaaagg gcggaggcgc gggataaact tcgggaaagc
      661 gcattatgcc ggcaaaaagt ctagggcgcg ctgggtgtgg aggagttgga ggtagccgag
      721 gacgtccgat tcgactttgt gggggtcagc tttggagtag agcttttgca gttcggtgac
      781 gagttgcgcg acggtgtgcc tgccgtcgca gcgggaaacg atttcgaggc tggggccgtt
      841 gaggcggagg gcgcgttcgg gcatgagcag gactctttgg ccttggtttt caccgaggcg
      901 cacgccgggg gcgagttggg gcttagcgtt gggatcgacg gacatgagcg atgaagagtc
      961 taccaccttc cgttaggcgt aagaaagaaa aagagagatg agcgagaatc ctcaaacggc
     1021 gacaggcgct cgatcgcgat tttccgaacc gtcccacgtc gtcagaatcg agaccaaaaa
     1081 aaatagtgca atggagagat tagtgaatcc tcctgccaag atcgcgccga acggcgcggg
     1141 cgttcggtcg gcaaggggag aaagcaaggc gaggactgcg ggattcgcca gcgcggtcca
     1201 cctggggtag ttactcttgc caaagaggac gagaccgatc aacaggattg cccctacgta
     1261 tcctggcaca gcgccaatat tgtatgcgat gttccagtag gagttgacgg cttgcagaag
     1321 agcgcggcag ctcgcgtcat cattgccata gcaagacttc aacgcaaggc ctttggctgt
     1381 ccagagcgtg tggatggcac tcccaaaaac catcaagatg gtgaaggcca atagtacgac
     1441 gcgaccgagc caaacatgcg gtgtccgcgc gtttaaatac gcgggccaga aaccaaagga
     1501 tgcacaggca agccgcgaag ggcccgacta atcctccagc atacaggcgg gcaagtgagg
     1561 ccgatcgaac gacagagacc attccgtcat gaaatgctgg acctgaaccc cagtgcccgt
     1621 aaaagagcat atcgcctgcg aagaagaata gcgccccaca gagcccggcg attccggcaa
     1681 gccgcttttg gagcagctcg tttggcacgt gggtcattgt ttgtctgggt ggaaggcacc
     1741 aggagggggc cagccgggct ggacgtaggc gtaatagagg gcgtcgagca tggcccagag
     1801 gatatcgcat ttggcgcgta gcgcggcgtg ggcggcgtct tgttcttcgc gggtgcgggc
     1861 atgtttgacg acccaggaga gagcgaactc ggcgtcttcg ggagcttgcg tgaggcggcc
     1921 ggtgaagtag tcgaggccgg aagagagcca cgggtagaat tcgcggagtt tttccatccg
     1981 gagagcgatg aggcgtttgg agaaaagttc ggtgagggaa gaggcgacgg cttcgaggtg
     2041 cgagcgggtg gagacgaagt ggatgtaggc gtcaacggca tagcgtgtgg cggggaggat
     2101 gccgtcgccg tgaacggcag acacgcggga gatgccggtg gcttcgacga gcttgagcca
     2161 cttttcgata ccaccgggaa gcggaccatc gggaccggaa tcggccgcgt cgccgtcgtg
     2221 atcgacgagc cgtttgcgcc agacggtgcg gaaagtgtgg tcatcggtac gtgcgagaag
     2281 agcggcgtct ttgcgcggaa tattggattg gtagtagtag cgattgagag cccaggcctg
     2341 aagctggccg cgagtgagcg cgccttggtg catcagtttg tgaaagggat gcttatggtg
     2401 gtagcgctct tcgccgatgg cgtggaagcc ggcgatgaat tcctcttccg agagtggatg
     2461 tttttcttgc gggtagttca cagagagatc tccataccat ccgaggctat ttcaaaaccg
     2521 gcgtcgcgga cggcgcggtg ctcggcggaa ttctcgtcca ggatgggatt ggtgttgttg
     2581 atgtggatga ggatcttgcg ggcggcggag tcggaaggga attgggcgag gaggccgtca
     2641 cgtccggaaa gcggaaggtg gcccatttcg cgggcgtttt tttggccgcg gccggtggag
     2701 aggagctcgt cgtcggacca gaaggtgccg tcgaggaagg cgacggtgct ggtggcggcg
     2761 gctttgagcc attcgccgga aatttcgggg agcgaaggag cgaagaagaa agatttgccg
     2821 gactgctcga aacggagagc gatcacggac tcctgggaag agagcgagcg gcggagttcg
     2881 ctgctcatgt agtcggggaa gccgccgcct acagagatgg gagaacagat gaaggtgggc
     2941 ggagccgaag ggtcttcgcc gaaacggcaa gcgaggcggc tgccaggcgt tagcgattgc
     3001 cattgaaccg gcggatcggc gcggtgaaga acgtcaaaga tacgattctc ggtttgcacg
     3061 attttttgga ttgcgggcgt ggcaaagaga aagaaggatt gaaattcgcg aagatggagt
     3121 aggcccatga gggagtcgac atcggcggac gggaggaaga cgccaccgat gggagtgccg
     3181 gtgggttcgc cgtcgggcgg tgccagctct ggagcggcta ggatttgcgt gcg
//
LOCUS       DASTPM010000447         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1082287, whole genome shotgun sequence.
ACCESSION   DASTPM010000447 DASTPM010000000
VERSION     DASTPM010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082287"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFR42_07900"
     CDS             <1..348
                     /locus_tag="VFR42_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369115.1"
                     /translation="WVPANAQGQKGDHAVTAGQLRQDVQKAADTRQANEAAVREMFAS
                     DQAKETLKSAGIDYQQVDQAISQVNDEDLSRMAQRSHEVKNDFAAGRLGDRDLLIILL
                     VAVAIILIIVAVR"
     gene            414..938
                     /locus_tag="VFR42_07905"
     CDS             414..938
                     /locus_tag="VFR42_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEU0369116.1"
                     /translation="MAGTLAAACCVLAAASDSGLWLDVPYVHQEKDGCGSASLSMVMQ
                     YWKGKNFAVAEDRVDPVRIQHELYAKKPRGIYASDMENYLRDTGFEVFTFRGEWSDLR
                     AQVAKGRPLIAGLKPKGAPAHYVVVVGVDPQDAAVLVNDPERGKMVRIERREFEKAWQ
                     RTETWTLLAVPRHS"
     gene            952..2646
                     /locus_tag="VFR42_07910"
     CDS             952..2646
                     /locus_tag="VFR42_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369117.1"
                     /translation="MKIAALFCAVFFFVGWAPPCIASDDGQRLKRVKKLYSEQKWEEA
                     AREAQGPAAQSPELDYYAGMALARLERWNEAEQVFSAGARKSPTDARFLTERAGAEYK
                     LNDFRAAKKDLQGALRLAPDDWYIPEFLGTIYLLEGNLEAALKYWNRLEKPRLTAVEI
                     APPARTENILLDRAVLFAPPQTLQRDTLLKTDALLENLGVFPRWRTELTPNGDDGYKA
                     TLKLTERSGWGANAIDGAISLLRGLPYETVYPSWTGIDGGAVNFDSLVRWDSEKRRIA
                     ANLEFPLFQQPARRVRVFFDQRNENWNLSNSLFGGTTAVSDLNLRRFSGGAELHAVES
                     GWWDWTAGVQAVSREFRNMPSDLPSSATPFFTGGRSLDAHLRIHRCLLRIPERRFAVE
                     GTGEVRGGRNYAAGLGAFGSVKGDVSARWLPKARSEDLEFVSQLRGGDTFGDVPLDLL
                     YELGVERDNDLWMRGHDGTTDGRKGNAPLGRRYILWNSELNKNVYDSGYFRVQVGPFF
                     DTGAIADPSGLFGSQKWLYDTGVQAKVRILGSVSVVLSYGRDLRNGKGLFYVHTAR"
     gene            2743..>4304
                     /locus_tag="VFR42_07915"
     CDS             2743..>4304
                     /locus_tag="VFR42_07915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU0369118.1"
                     /translation="MRSSFLLRRIFAVTILIAGSFGVLRASGRADGSGWGFSEANLDR
                     SCKPCDDFNQFAMGGWMKNNPIPAEYPNWGSFTMLADRNQASMRVILEEAAKANAASG
                     SNQQKIGDFYSSCMDTAAVDAAGVKPLVADFTAIDQVKDAKELQPLIARLQQTGSGYL
                     FRFDSTQDLDESTQVIAEINQGGLGLPDRDYYTRTDEKSAQLRTDYIAHVAKMFALAG
                     DTEETANAEAKTVMDLETALAKASMSRVQMRDPHAVWHKMTLPQLKELAPGWAWEAYF
                     RQRNAPEFASINVSQPDFFKETNRLLTATPLKDWKTYLRWHVLHASATQLSEPFVQEN
                     FNFYGTKLSGTKQLQPRWKRCSQSVNRDLGEALGQVYVEKYFPPEAKAHARVMVMNLI
                     GALKSDIPELSWMGPDTKKAALEKLEAFQIKIGYPDKWRDYSALTVDKGSYLANERRA
                     IAFENARDLNKIGKPVDRSEWQMTPPTVNAYYDATMNEIVFPAGILQPPFYDAKADDA
                     VNYGGMGAAIGH"
BASE COUNT          943 a         1081 c         1450 g          830 t
ORIGIN      
        1 tgggttccgg cgaatgcgca ggggcagaag ggggaccatg ccgtaaccgc gggccagctg
       61 cggcaggatg ttcagaaagc ggctgacacg cggcaggcga acgaagcggc cgtacgcgag
      121 atgttcgcct cggaccaggc caaagaaaca ttgaagtcgg cagggatcga ctaccagcag
      181 gtagatcagg ccatcagcca agtgaacgac gaagacctat cgcgtatggc gcagcgttcg
      241 cacgaggtga agaacgattt cgccgctggg cgcctgggcg atcgcgactt gctgattatc
      301 ctgctggttg cggtggcgat aattctgatc attgtggcgg tacggtagac cgaagttccg
      361 cttcgatgac tcggcaaccg ggggcagtcc tgaaaatcgc aagacagttc gtaatggcgg
      421 gaacgcttgc cgcggcatgt tgcgtgcttg ccgcggcaag cgattccggc ttgtggctgg
      481 atgtgccgta cgtgcaccag gagaaggatg ggtgtgggtc ggcgtcgctg tcgatggtga
      541 tgcagtactg gaagggcaaa aattttgcag tggcggaaga ccgggtggat ccggtccgga
      601 ttcaacacga gttgtacgcg aaaaagccgc gcgggattta tgcttcggat atggaaaact
      661 acctgcgcga cacgggattt gaggtgttta cgtttcgcgg cgagtggagc gatttgcggg
      721 cgcaggtggc caaggggcgg ccactgattg cggggctgaa gccaaaaggc gcgccggcgc
      781 actacgtggt agtggtggga gtggatccgc aagacgccgc agtgctggtg aacgatcccg
      841 aacgcgggaa aatggttcgc atcgagcggc gcgaatttga gaaagcgtgg caaaggacgg
      901 aaacctggac gctgctggcc gtgccgcgtc attcctagtg aaacattcac agtgaaaatc
      961 gctgcgttgt tctgcgccgt atttttcttt gtgggatggg cgccgccgtg cattgcgagc
     1021 gatgacgggc agcggctgaa acgggtcaag aaactctatt ccgaacaaaa gtgggaagag
     1081 gcggcgcgcg aagcacaggg tccagcagcg cagtccccgg aattggatta ttacgccggg
     1141 atggcgttgg cacggctgga acgatggaac gaggccgagc aagtattttc ggccggcgcg
     1201 cgaaaatccc cgaccgatgc gcgctttctg accgaacgcg ccggcgcgga atataagctg
     1261 aacgattttc gcgcagctaa gaaagatctg cagggcgctc tgcggctggc gccggacgat
     1321 tggtacatcc cggaatttct cggaacgatt tacctgctcg aaggcaacct cgaagccgcg
     1381 ttgaaatact ggaacaggct ggaaaagccg aggctgacgg cggtggagat cgcgccgccc
     1441 gcaagaacgg aaaacatctt gctggaccgg gccgttctgt ttgctccgcc gcagacattg
     1501 caacgcgata cgttgctgaa gaccgatgcg ctgttggaaa acctgggcgt gtttccgcgc
     1561 tggcggacgg aattgacacc caatggagac gacggataca aggcaaccct aaagttgacg
     1621 gaacgcagcg gttggggtgc gaacgcgatc gatggcgcaa tctcgctact gcgcggcctg
     1681 ccttatgaaa cagtttatcc gtcatggact ggaatcgacg gaggggcggt gaatttcgat
     1741 tcgctggtgc gatgggattc cgagaagcga agaatcgcag cgaatctgga gtttccgctg
     1801 tttcagcagc cggctaggcg ggtgcgggtg ttttttgatc agcgtaacga gaactggaat
     1861 ttgtcgaaca gtttgtttgg cgggaccacg gcggtcagcg atctgaacct gcgccgattc
     1921 tccggcggcg cggagttgca cgcggtagag agcggatggt gggattggac ggcgggtgtg
     1981 caggccgtct cacgcgaatt tcgcaacatg ccgagcgatt tgccaagcag cgcgacgcca
     2041 ttctttaccg gaggccgttc gctagatgcc cacctccgta ttcaccgatg tttgctgcga
     2101 attccggagc gccgattcgc ggtggaggga acgggcgaag tccgcggagg ccgaaattat
     2161 gccgcggggc tgggagcatt cggctcagtg aagggcgacg taagcgctcg ctggctgccg
     2221 aaagcgcgca gcgaggatct tgagtttgtg agccaactgc gcggcggcga tactttcggc
     2281 gacgtgccgc tggatttgct ttatgaactt ggggtcgaac gggacaacga tctgtggatg
     2341 aggggccatg atggaaccac ggacgggcgc aaggggaatg cgccgctggg acggcgctac
     2401 atcctatgga actccgaact gaacaaaaac gtctatgaca gtgggtactt tcgcgtgcag
     2461 gtggggccgt tttttgacac cggggcgatt gcggatccga gcgggctatt tggttcgcaa
     2521 aagtggctat acgacacggg agttcaggcc aaagtgcgga ttctgggaag cgtgtcggtt
     2581 gtcttgtcct atggacggga cctgcgcaac ggcaaaggac tgttttacgt gcacacggca
     2641 cgctgagatt ttgacgtttc gatcgagccg gttggccagc cgtttttgtt aaactaagtc
     2701 ggtttgggcg ggcgagtttt tcgccgccgt ctggggagac acatgcgatc ttcattcctg
     2761 ctgcgccgaa ttttcgcggt taccattctg attgcgggga gcttcggagt cttgcgcgcc
     2821 agtggccgcg ctgacggttc cggctgggga ttcagcgaag cgaacttgga ccgttcgtgc
     2881 aagccctgcg acgacttcaa ccagttcgca atgggcggtt ggatgaagaa caatccgata
     2941 ccggcggagt atccgaactg gggttcgttc acgatgctgg cggaccgaaa ccaggcgtcg
     3001 atgcgtgtga ttctggaaga ggcggccaag gccaacgcag cgtcgggctc gaaccagcag
     3061 aagatcggcg acttttattc gagctgcatg gatacggcag cagtcgacgc agccggcgtg
     3121 aagccgttgg ttgcggactt cacggcgatt gaccaggtga aggacgcgaa ggagttgcag
     3181 ccactgatcg ccagactgca gcagacgggc agcggctacc tcttccgttt tgactcgacg
     3241 caggacctgg acgaaagcac gcaggtgatc gcggaaatta atcaaggcgg actagggctg
     3301 cccgaccgcg attactacac gcgcacagac gaaaaatccg cgcagctacg gacagattac
     3361 atcgcgcatg tagcgaagat gtttgcgctg gcgggtgaca ccgaggaaac ggcaaacgcg
     3421 gaagccaaga cggtgatgga cctggagacc gcgctggcga aggcgtccat gtcgcgggtg
     3481 cagatgcgcg atccgcatgc ggtgtggcac aaaatgacgc tgccacaact gaaagagctg
     3541 gcgccgggct gggcttggga agcgtacttc cggcagcgca atgctccgga atttgcttcg
     3601 atcaatgttt cgcagccgga ctttttcaag gaaacgaacc ggctgttgac agcaactccg
     3661 ctgaaggact ggaaaaccta tctgcgctgg catgtgctgc acgccagcgc cacccagctc
     3721 tccgagcctt tcgtgcagga gaattttaat ttttacggaa ccaagctctc gggtaccaag
     3781 caactacagc cgcgctggaa gcgctgttcg caaagcgtga accgcgacct cggcgaagcg
     3841 ctgggccagg tttatgttga gaaatatttt ccgccggaag ccaaggccca cgcgcgcgtg
     3901 atggtaatga atttgatcgg cgcgctaaag agcgacattc cggagctgag ctggatggga
     3961 ccggatacga aaaaggccgc gttggaaaaa ctggaagcgt tccagatcaa gatcggttat
     4021 ccggacaagt ggcgcgacta ttcagcgctc accgtggaca agggaagcta tctggcaaac
     4081 gagcggcggg cgattgcgtt tgagaacgcg cgcgacttga acaagatcgg caaacccgtg
     4141 gatcgcagcg aatggcagat gacgccgccg acggtgaatg cgtactacga tgcaacgatg
     4201 aacgaaattg tgtttccggc gggaattttg cagccgccgt tttacgatgc aaaggcggat
     4261 gatgctgtga attacggagg catgggcgcg gccatcggac acga
//
LOCUS       DASTPM010000448         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_275475, whole genome shotgun sequence.
ACCESSION   DASTPM010000448 DASTPM010000000
VERSION     DASTPM010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275475"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2314)
                     /locus_tag="VFR42_07920"
     CDS             complement(<1..2314)
                     /locus_tag="VFR42_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0369119.1"
                     /translation="MGKFLGELDSALQNFTHPVVHRELKWDSSRAAWIRDQMHHLEES
                     SRRALMEYFISLYQEDVEPRRDELRKSAIYGDANDHNVLVSPPWPQPRKVAGVIDFGD
                     MHYGWTVSEAAIAAAYAILGKENPLSVVAAIARGFHGAFPLSEPEIAALFPLIGMRLA
                     VSVVNSAVRKKQRPGDPYVTVTEDQAWPALERLAQIHPRFAQYMLRDACGLPAVPKER
                     KVASWLEALRAKGASVLEADLRTAPLHVFDLSAGSAMLGADPANAEAHSLTRAIFERM
                     DEERVAIGVGRYNEARLLYTAAQYGGDAAHANERRTIHLGLDLFVVPGTAVCAPLDGT
                     VHLLAENTAELDYGPLLIMRHVTGDGEEFFTLYGHLSRETLRELKPGQAIARGEIFAH
                     VGTLHENGGWPPHLHFQVILDLLDRGAEFAGVARASERSVWTSLCPDPNLLLGIPAEQ
                     IPALENSAHETLPKRRELLGGNLRVSYRKPLKVVRGWRQHLYDESGRPYLDVYNNVPV
                     AGHSHPRVVKAAREQLALLNTNTRYLHENILRYAERLTALLPEPLRVCYIVNSGSEAN
                     ELALRLARAETRCDDTIVLEHAYHGHTNTLIDISPYKFGGPGGTGKKSRVHVAPLADD
                     YRGIYRRGEAHLGAKYAKHVGEICGELQSQGKRPTYIAETLPSVGGQIVFPPDYLGEV
                     YRHVRATGGVCIADEVQVGFGRLGTHFWGFETQRVVPDIVVFGKPIGNAFPLAAVVTT
                     REIAREFDNGMEFFSTFGGNPVSCAVGLAVL"
BASE COUNT          472 a          756 c          649 g          479 t
ORIGIN      
        1 cgagtaccgc gagccccacc gcgcaggaaa cgggattgcc gccgaatgtg ctgaaaaatt
       61 ccatgccgtt atcaaactcg cgcgcgattt cacgtgtggt gaccaccgcg gccagcggaa
      121 acgcatttcc aatcggcttg ccgaacacca cgatgtccgg cacgacgcgc tgcgtctcga
      181 acccccaaaa gtgcgtgccg agccggccga atcccacttg cacctcgtcc gcaatgcaca
      241 cgccaccagt cgcgcgaacg tggcgatata cttcgccgag gtaatcgggc ggaaaaacaa
      301 tttgcccgcc gacgctcggc aaagtctccg cgatgtaggt tggtcttttc ccctgagatt
      361 gcagctcacc gcaaatttct ccgacgtgtt tcgcgtattt cgctccgaga tgagcctcgc
      421 ctcggcgata gattccgcga tagtcatcag ccagcggcgc gacatgtacc ctcgatttct
      481 tgccggttcc tcccgggccg ccgaatttgt acgggctgat atcaatcagc gtgttggtat
      541 gcccgtgata cgcgtgctcc aggacgattg tgtcatcgca tcgcgtttcc gcacgggcca
      601 gccgcagcgc cagctcgttc gcttcgcttc ccgaattcac gatgtagcac acgcgcagcg
      661 gttcgggcag gagtgccgtc agcctttcgg cataccggag aatattttcg tgaaggtagc
      721 gggtattcgt attgagaagc gccagctgtt cccgcgctgc tttcacaacc cgaggatgcg
      781 aatgccccgc gaccggaacg ttgttgtaca catcgagata cggacgcccg ctttcgtcat
      841 acaaatgctg ccgccacccg cgcacaactt tcagcggctt gcggtaggaa acacgcaggt
      901 tcccgccgag tagctccctt ctcttcggca gtgtttcgtg cgccgagttt tccaaggctg
      961 gaatttgttc cgcagggatt cccaacagca aattcggatc ggggcacaaa ctggtccata
     1021 ccgaccgctc cgacgcgcgt gctacgccag cgaattccgc gccgcgatcc agcaagtcca
     1081 ggatcacttg aaaatgcaga tgcggcggcc agccgccgtt ctcgtgcagc gtgcccacat
     1141 gagcaaaaat ctcgccgcgg gcaatcgctt gtcccggttt cagctcgcgc agagtttcgc
     1201 gcgacaaatg gccatacaac gtgaagaatt cttcgccgtc gcccgtgacg tgccgcataa
     1261 tgagcagcgg gccgtagtcc agctccgccg tattttccgc cagcagatga accgtgccgt
     1321 cgagcggagc gcatacggcc gtccccggca caacaaatag gtccagcccc aggtgaatgg
     1381 ttctccgctc gtttgcgtgc gcagcatccc cgccatattg agccgcggta tagagcaagc
     1441 gcgcttcgtt gtatcgtcca acgccaatgg ccacccgctc ttcgtccatg cgctcaaaaa
     1501 ttgcccgcgt aagcgaatgt gcttccgcgt tggcggggtc ggccccaagc atcgcgctgc
     1561 cggcgctcaa atcaaaaacg tgcagtgggg ctgtgcgcag atccgcttcg agaacgctcg
     1621 ctcccttcgc ccgcagcgct tcgagccaag atgccacctt ccgctctttt ggcaccgcgg
     1681 gaagcccgca agcgtcgcgt agcatgtatt gcgcgaatcg cgggtgaatc tgcgcaagcc
     1741 gttccagcgc aggccacgcc tgatcctcgg tgacggtgac atagggatcg ccgggcctct
     1801 gtttcttacg cactgccgaa ttgacgacac ttaccgcgag ccgcatcccg atcagcggaa
     1861 acagcgcggc gatttccggc tcactcagcg gaaacgcacc gtggaatcct cgtgcgatgg
     1921 cagccacgac gctcagcgga ttttctttac ccaaaatggc gtatgccgcc gcgatagccg
     1981 cctcgctgac cgtccagccg taatgcatgt cgccaaagtc gatcacaccg gcaaccttcc
     2041 gcggctgagg ccacggaggg ctgaccaaaa cgttgtggtc attcgcgtcg ccgtagatcg
     2101 cgctcttgcg cagttcgtcg cggcgtggtt cgacgtcctc ttgataaagc gaaataaaat
     2161 attccatcag cgcgcgacgc gacgattcct ccaagtgatg catttgatcg cgaatccatg
     2221 cggcgcgcga cgaatcccat ttcagctccc ggtgaactac cggatgggta aagttctgta
     2281 acgcggagtc tagttcgcca agaaactttc ccaaaccttc caagatctct ggcgaatgag
     2341 gattggtctt ctccag
//
LOCUS       DASTPM010000449         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1140158, whole genome shotgun sequence.
ACCESSION   DASTPM010000449 DASTPM010000000
VERSION     DASTPM010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140158"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..307)
                     /locus_tag="VFR42_07925"
     CDS             complement(23..307)
                     /locus_tag="VFR42_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEU0369120.1"
                     /translation="MAKAFWAGTLIAESNACVVVEGNQYFPEDAVKKQYLKSSNHTSI
                     CPWKGTAHYFHVEVDGMRNDNAAWFYPDPKPEAAKISGRIAFWKGIRIES"
     gene            427..2145
                     /locus_tag="VFR42_07930"
     CDS             427..2145
                     /locus_tag="VFR42_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369121.1"
                     /translation="MLIRRISSFGIVALCLTICAELISCKPGEKVSAPAKIASIAAPL
                     GVAPDLDARLAKFKAIEMPLNRGALSPKEVQLVQKLVDAANSIESIYWRQSDPDGLKL
                     YGELEKSADPLDQKVLRFLKINGSRYDLIDELKPFIGTQPAPPGRALYPVGLTREEIE
                     KYAAANGEKQMVYDEHSVLRKVEAKFQATPYHVAYAEFLRNAAQDLQEAAALSDDAAF
                     AKFLRLRSDALLSDDYYKSDLAWLDLKEPKFDLILAPYESYLDNLLGVRTSYGAAVLI
                     RNEEESKKLAIFQKYVPELQEALPLDKRDLPSKRGHVTPMEVMDAPFRTGDLLHGYQS
                     VADNLPNDARVHEKKGSKKIFFKNFMDARVNVVIVPIARRLLREDQALQATGDGYLAS
                     TLAHEISHELGPNYSRTAAGKRDIREAIGKDFSALEEAKADVAGMFCLKWLADHGAIT
                     KAQLEEDYISYVAGNFRTIRFGIAEPHGRGEMMEFNYMLEHGAVRRDAATGRYAVMLD
                     KMPGAIASLAKELLEQEATGDGARTAAWFAKYAVLPDHLQRAFEQISDIPVDIQPIYS
                     FSEDVR"
     gene            2142..2600
                     /locus_tag="VFR42_07935"
     CDS             2142..2600
                     /locus_tag="VFR42_07935"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HEU0369122.1"
                     /translation="MREPQNFSNHTKIVPAFHYFLLPALFINLVGASYWVYATARHAW
                     GPFPLFYAILNLFLAIALLILAFLARLFALGVQDRVIRLEERLRYQQLLPEDLKARIN
                     EFTTRQLVALRFASDTELPALARKVLDEKVKERKAIKQMIQNWRADYQRI"
     gene            complement(2614..>2848)
                     /locus_tag="VFR42_07940"
     CDS             complement(2614..>2848)
                     /locus_tag="VFR42_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0369123.1"
                     /translation="ASEGVADFVQNMAKSRGVSSAQVEKEFFETIRPSSLLKRFETTE
                     EVASIVAFIASSQSVGINGAAVRAEGGVVRSIL"
BASE COUNT          656 a          744 c          817 g          631 t
ORIGIN      
        1 agcaacaaat agagagatcc aattacgact cgatgcgaat gcctttccaa aaggcgatgc
       61 gtccggagat tttcgctgct tcaggcttag gatcaggata aaaccaggcc gcattgtcgt
      121 tgcgcatgcc gtccacctcg acgtggaagt agtgcgcagt tcccttccac gggcagatcg
      181 atgtgtggtt gctggatttg agatattgtt tttttactgc gtcctcgggg aaatattggt
      241 ttccctcaac gaccacacag gcgttactct ccgcaatcag tgttcccgcc cagaatgcct
      301 tggccatgag actctccgtc atccgttgga tgcccgccgg ctggaaaagt ttcgcaagcc
      361 gctattcact tcagtttatt ggcagcgttt cgcggagtgc aagcctcgtt tgctacaatt
      421 ttgcgcatgc tcatcagacg aatctccagc ttcggaatag ttgccctctg tttgaccatt
      481 tgtgccgaat tgatctcctg caaaccaggc gaaaaggttt ccgcgccggc aaaaatcgcc
      541 agtattgccg cgccgctcgg cgttgcaccg gatctggatg cgcgcctggc caagttcaag
      601 gcgatagaaa tgccactgaa ccgcggtgcg ttgagcccga aggaagttca gctagtgcag
      661 aagctggtcg acgccgcaaa ttcgatcgaa agcatttact ggcggcagag cgatccagac
      721 ggtttaaagc tttatggcga acttgagaag agcgctgatc cgctggacca gaaagtcctg
      781 cgcttcctga aaatcaacgg cagccgctat gacctgatcg atgaactgaa gcccttcatc
      841 ggcacgcaac cagcgccgcc ggggcgtgcg ctgtatcccg tggggctaac gcgcgaagaa
      901 attgagaaat acgccgcagc caatggcgag aaacaaatgg tctacgacga gcattcggtc
      961 ctgcggaagg tcgaggccaa gtttcaggca actccctatc acgtggctta tgccgaattt
     1021 ttgaggaacg cggcgcagga tctacaggaa gcggcggcgt tgagcgatga cgccgcgttt
     1081 gcgaaattcc tgcggctgcg ctctgacgcc ttgctcagcg atgactacta caagagcgat
     1141 ttagcgtggc ttgacctgaa agaaccgaag ttcgacctga ttttggcacc ctatgaaagc
     1201 tatctggata acctacttgg cgtccggact tcgtacggcg ccgcagtgtt gattcgaaac
     1261 gaagaggaga gcaagaaact cgccatcttc cagaaatatg tgccggaact tcaggaggcg
     1321 ctgccgctgg acaagcggga cctgccgtcg aagcgcggac atgtgacacc gatggaagtg
     1381 atggacgcgc cgtttcgcac cggagatttg ctgcatggct atcaatcggt ggcggacaac
     1441 ctgccgaatg atgcgcgggt gcacgaaaag aagggcagca aaaagatttt cttcaagaat
     1501 ttcatggatg cgcgcgtgaa cgtcgtcatt gtgccgatcg cacggaggtt gctgcgcgaa
     1561 gatcaggcgc tgcaagcgac gggtgacggc taccttgcaa gcacgctggc gcatgaaatt
     1621 tctcatgagc tggggccgaa ctattcgcgc acggccgcgg gtaagaggga catccgcgag
     1681 gccattggaa aagacttttc cgcgctggaa gaggcgaaag cggatgttgc cggaatgttt
     1741 tgcctgaaat ggctggccga ccacggagcg attacgaagg cgcagttgga agaggactac
     1801 atttcctatg tggcaggcaa ttttcgcacg attcgctttg ggatcgccga accgcatggc
     1861 cgcggagaaa tgatggagtt caactacatg ctggaacatg gggccgtgag gcgggatgcg
     1921 gcgacgggcc gctacgcggt gatgctggac aagatgcccg gcgcgatcgc atcgctggca
     1981 aaagaattgc ttgaacagga agcgaccgga gacggcgcgc gaactgcggc gtggttcgca
     2041 aaatacgccg ttttgcccga ccatctccag agagccttcg aacaaatttc cgatatcccg
     2101 gtggatattc agccgattta ttccttctcg gaggatgttc gatgagagaa cctcagaatt
     2161 tttccaatca cacaaaaatc gttccagcat ttcactattt tcttttgccg gcactgttca
     2221 taaatttggt gggcgcaagc tattgggtct atgcgacggc tcggcatgcg tggggaccct
     2281 ttcccttgtt ctatgcgatt ctgaatctgt tccttgcgat cgctctgctt attctggctt
     2341 ttcttgcgcg actcttcgcg ctcggtgtgc aggaccgcgt gattcgattg gaggaacgcc
     2401 tgcgttacca gcagctgctt cccgaagact taaaagcgcg gatcaatgaa ttcacaacca
     2461 ggcagctggt tgcgttgcgc tttgccagcg acaccgagct gccagcactg gccaggaaag
     2521 tcctggatga aaaagtgaag gagcgcaagg ccatcaagca gatgattcag aattggcgcg
     2581 ccgactatca gcggatttaa gccgccggcc aggttaaaga atactgcgga caactccgcc
     2641 ttcggcgcga acggcagcgc cgttgattcc taccgattgg gagctggcaa tgaacgcaac
     2701 gatcgatgcg acttcttccg ttgtctcaaa gcgcttcaac aaagagcttg gccggatggt
     2761 ttcaaagaat tctttttcga cctgagcgga acttacgccg cggctcttcg ccatgttttg
     2821 cacaaaatcg gcaacgcctt cggatgca
//
LOCUS       DASTPM010000450         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1140168, whole genome shotgun sequence.
ACCESSION   DASTPM010000450 DASTPM010000000
VERSION     DASTPM010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140168"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VFR42_07945"
     CDS             complement(<1..1032)
                     /locus_tag="VFR42_07945"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU0369124.1"
                     /translation="MSGSFPKQRLIELDGLRGLALIMILVFHSVSQEGTYPAGTFLAY
                     LQRSMGMGWTALDLFFVLSGFLIGGILMDARDSPAYFKTFYARRFFRIVPVYFAWVLL
                     YVLVAGLAADLVVKFSNSGLRPPLDFSVWSHFLFLQNSFDFHYLGLAGAWFGHLWSLA
                     VEEQFYLVTPLVVRMVPPRYLKRILVVVVLLAIGARIYFRFVSHMPGIMVTTRTLSRM
                     DALAIGMLAAILVRSESGRLWLDQNLAALRAILGLLTTGVIVFFFFSYGSLTRGMQTI
                     GFTWMAAFYAAVLLLAVQNRSGWLAGFLRMPLLLELGSVSYCVYLVHLVVNVSLHALL
                     LHHSPQISTR"
     gene            complement(1203..1583)
                     /gene="trxA"
                     /locus_tag="VFR42_07950"
     CDS             complement(1203..1583)
                     /gene="trxA"
                     /locus_tag="VFR42_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU0369125.1"
                     /translation="MGSRIAGNSLIAKNMADNNIQSVNDGNFEAEVLTASGAQPVVVD
                     FWAEWCRPCHMLAPTVAEIAHDYAGKIKVTKLNVDEAMNSAGRYNIRGIPTILVFKGG
                     QVVEQIVGAVPKDQITKAIDRHVG"
     gene            complement(1599..>2014)
                     /locus_tag="VFR42_07955"
     CDS             complement(1599..>2014)
                     /locus_tag="VFR42_07955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0369126.1"
                     /translation="NVVAHKVEAGSDIFLMPSRYEPSGLNQMYSLKYGTVPVVRATGG
                     LDDSIENFDLEHGTGTGFKFSEYSGTAFLYAVRQALHHYSDERIWKRIQLNGMGKDFS
                     WKTPAAEYARLYGAARAARGLAQPAGNQLPVATSN"
BASE COUNT          523 a          554 c          587 g          350 t
ORIGIN      
        1 gcgtgtcgag atttgcggcg aatgatggag cagcaaggcg tgcagcgata cgttgacgac
       61 gagatggacc aaatacacgc aataagaaac cgagccgagc tccagcaaca gaggcatacg
      121 caaaaagcca gccagccagc cggagcgatt ctgcacggcc agcaggagca ccgccgcata
      181 gaaggcggcc atccaggtga aaccgatggt ttgcatgccg cgggtcaagg agccgtacga
      241 aaagaaaaag aacacgatga cgcccgtcgt caacaaaccg aggatcgcgc gtagtgctgc
      301 gagattttga tcgagccaga ggcggccgct ttccgaacga accaggatcg ccgcgagcat
      361 gccgatggcg agcgcgtcca tgcgggaaag cgtgcgcgtg gtgaccatga ttccgggcat
      421 gtgggaaacg aaacgaaaat aaatgcgggc cccaatggca agcagcacca ccacgacgag
      481 aatccgtttc aggtaacgcg gggggaccat gcgaaccacc agaggcgtga ccagataaaa
      541 ctgttcttcc accgccagcg accacaaatg gccgaaccag gcgcccgcta atcccaggta
      601 atggaagtcg aacgagttct gcaagaacag gaaatgcgac cagacggaaa aatctagcgg
      661 cggccgaaga ccggaattcg agaacttcac gaccaaatct gctgcaagac cggcgaccag
      721 aacgtaaagc aggacccacg cgaagtaaac aggaacgatg cgaaaaaaac gacgagcgta
      781 gaaagtcttg aaataagcgg gcgaatcccg cgcgtccatc aagatgccgc caatcagaaa
      841 gccggagagg acaaagaaga gatccagcgc cgtccaaccc atgcccatgg aacgctgcaa
      901 gtaagcaagg aatgtgccag cggggtacgt cccttcctgc gaaacagagt ggaacacaag
      961 gatcatgatg agagctagtc cgcgtaaacc gtcgagttcg atgaggcgtt gcttggggaa
     1021 agagccggac attcgcgtct gggcaaagta gcaagggcga ggagcggggt caaggaaggc
     1081 cggacggcgg gcgaaggacg gcgggcgaag gagttgacaa aaaaagaaga gcgcacgagc
     1141 ctggaacccg cgcgcccttc tagaaacgct tttgccagcc aagaaaccta cggaagcgaa
     1201 gctcaaccga cgtggcgatc gatggctttg gtgatctgat ccttgggaac cgcaccgacg
     1261 atttgctcga ccacctggcc gcccttaaac accagaatcg tgggaatgcc gcgaatgtta
     1321 tagcggccgg cggagttcat cgcctcgtcc acgttgagtt tggtgacttt gatcttgccc
     1381 gcatagtcgt gggcgatttc cgcgaccgtg ggggccagca tgtgacatgg gcggcaccac
     1441 tccgcccaaa aatcgacaac taccggctgc gcgccgctag cggtaagcac ttccgcttcg
     1501 aagtttccgt cattaaccga ctggatgttg ttgtctgcca tgttctttgc tatcagcgag
     1561 ttaccggcta tgcggcttcc cactggtctc cttgtccctt aattagatgt tgcaacaggt
     1621 aattggtttc ctgcgggttg cgccagtccc cgggcggcac gcgccgctcc atatagtctg
     1681 gcgtattccg ccgcaggtgt cttccaggaa aaatccttac ccataccgtt tagctggatt
     1741 cgtttccaga tgcgctcgtc ggagtagtga tgcaaggctt gcctcacagc gtacaaaaac
     1801 gcagttcccg agtactcact gaacttgaaa ccggttccgg tgccgtgctc gagatcgaaa
     1861 ttttcaatac tgtcgtcgag accacccgta gcgcgcacta ccggtaccgt accatatttt
     1921 aagctgtaca tctgattgag accgctcggc tcataacggg atggcatcag gaaaatgtcc
     1981 gagccagcct cgaccttgtg cgccaccacg ttgt
//
LOCUS       DASTPM010000451         8033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_188956, whole genome shotgun sequence.
ACCESSION   DASTPM010000451 DASTPM010000000
VERSION     DASTPM010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188956"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFR42_07960"
     CDS             <1..237
                     /locus_tag="VFR42_07960"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEU0369127.1"
                     /translation="RGYFPSTLNGLDVMVKDSKRFAETNNWGFFTFGHHAEPYETAAK
                     ESSISQCAYCHIANVAKTDMVWVQYYPLLKTKID"
     gene            529..1731
                     /locus_tag="VFR42_07965"
     CDS             529..1731
                     /locus_tag="VFR42_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0369128.1"
                     /translation="MITLAKYKYWIGGAFLLLVLGVIFGVALPKHRSDALGGPADVQI
                     VPVEQKDVQIYGEWIGTLDGLVNADVRAQVTGYLLRQDYQEGAIVKKGQLLFEIDPRP
                     FQASLDQAEGQLAQAKATLVNMEAVQRRTQLDVDRYTPLAKEQAASQQDLDNAVQNNL
                     AAKANVETAKAQIKTYEAAVETARINLEVTRLIAPIDGIAGQAQLQVGALVSPASGVV
                     TSVSTVSPIKVFFTASEPQYLEWRRRFPTETSTQAAAKALHLQLVLANGSTYAHEGTF
                     YFADRQINESTGAIRLAALFPNPESILRPGGYGRVRAVIRTQRDALVVPQRAVSELQG
                     GYQVAVVDGENKVSIRPVKVSDTVGSQYVIAEGLKPGDRVVAEGVQKVRPAMTVNPKP
                     FVAETKGE"
     gene            1736..4945
                     /locus_tag="VFR42_07970"
     CDS             1736..4945
                     /locus_tag="VFR42_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU0369129.1"
                     /translation="MSKFFINRPIVAMVISILMVIVGGITILALPVAQFPNIAPPEVQ
                     ILATYVGADAQTLEQAVATPIEQQMNGVDNMNYMYSLNATANSNTTLIVNFDVKTDPN
                     TDLILAQSRETQAASQLPADVNNYGITVRKSVTAPLMLLALYSPHGTYDATFLSNYAY
                     INLVDPITRSYGIGNVQVFGAGQYAMRLWVKPDQLAKLGITVTDIISAIQAQNTVNPA
                     GRAGGEPAPKGQEFTYSVLAQGRLISPGEFGNIVLRETPDGGVVRVRDVARIELGSQD
                     YSTSGRFNGKPSAIVAVYQLPGSNAVNAADNVLKLMGEMKHRFPADMDFATALDTTRA
                     VTEGMKEIVVTLFIALVLVILVVYIFLQGLRSTLIPLLAVPVALVGTFIFFPLFGFSL
                     NTLSLFGLVLAIGLVVDDAIVVVEGVERHIEEGMTPKNAALKAMEELSGPVVGIALVL
                     SAVFVPTAFIPGITGRLYQQFAVTIAISVLLSAFNALTLSPALAALLLRPKEPSRGLL
                     RRFFDWFNRTFERATEGYVRWSAVLIQKTVVMVVLLVGFCIAGGFFANRVPSSFLPDE
                     DQGYFYVNMQLPNSASLERTSDVAVDVEKILANTPGVRYTTSVIGFSLLSFVRTTYNA
                     FFFVTLKPWDERTSRAEQLQAIKAHLNRELSKVPAAIAFGFSPPAIPGVGTSGGFTFI
                     LEDRSGSDVQFLAKNLATFMEAARKRPEIAQLSTTFLPSVPQKFLDVDRDKVLKQGVV
                     LNDVYKTIQAFMGGYFVNYFNRFGRQWQVYIEAEGEDRAKAENVGQFYVRNNKGENVP
                     LSSLVTVKNRSGPEFTMRFNEYRSAQLNGGSAPGYSAEQATAALEEVFKQTMPREMGF
                     DYSGMSFQEQKAREGVPASVIFGISLLFVFLILAALYESWSLPFSVLLSTPVAVFGAF
                     AILWLRRVLLGFFVPPYMVQIETDVYSQIGLVMLIGLAAKNAILIVEFAKDQFEKGRP
                     LVDAALEGAKLRLRPILMTSFAFILGCVPLWTASGAGSVARQIMGTTVIGGMLAASSI
                     GIFFVPAIFYLVEKWSGAGKEPALGTLPAAPAAHPQPGD"
     gene            5006..6403
                     /locus_tag="VFR42_07975"
     CDS             5006..6403
                     /locus_tag="VFR42_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0369130.1"
                     /translation="MKLAKSLIPVVACAWLVGCKVGPNYRRPVVQTPTAYRDLSENPQ
                     LQAQAASYADLPWWQVFQDPQLQDLIRTALKQNYDLQLATERINAARAQVAITRSSLF
                     PQVAAGGTFTGGKESNFQTRFNFLTLAADATFQLDFFGRLRRATEAARAELLATQAAR
                     QTVILTLVSDVASDYFSLLQLDLQLQITHNTINAQTESVKLTKFRVDHGVATKLDVLQ
                     AQQTLDTANATVPDLERQIAQEEDAISILLGDYPHAVTRGLPLVDQLLPPAVPPGLPS
                     SLLERRPDIREAEQNLIAANAEIGVAKAQFFPQIELTGSGGGAFGRSSTFSSLMPSQL
                     GIWSYGAQVTQPIYTGGALKGNLHLAESQYRQLFIAYLQTIQQAFGDVSDSLIGYEKL
                     HQVRVRQEETVADLQEAVRLSNMRYKGGTTTFLEVLDGQRALFSAELTLAQARGSEYQ
                     SLVQLYRALGGGWQQ"
     gene            6853..7713
                     /locus_tag="VFR42_07980"
     CDS             6853..7713
                     /locus_tag="VFR42_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU0369131.1"
                     /translation="MKEAMEKKPTDESGVPGCPNRSDGGSESRRFHWPEYLMEAGELG
                     LFMFSVCVLATLFRHPSSPLSHLVVGELARRLFIGLATGAMVVMIVLTPWGKQSGGHF
                     NPAITFTFYRLGKVDAWDAIFYGVAQFAGAVSGVMLAVYLLRGAPSHDQVRYAMTVPG
                     RFGTVAAFLAEFAISFMLMTTILYATNHAAYARFTPYLVGAQYALYITFETPLSGMSM
                     NPARTFGPAFHASYWHALWIYFVAPTLGMLVSAEVFLHCRDGLRPYCAKLYHAPDKGC
                     IFRHDGPTVA"
BASE COUNT         1710 a         2324 c         2202 g         1797 t
ORIGIN      
        1 cgggggtact tcccaagtac gctaaacggt ctcgacgtga tggtgaagga cagtaaacgc
       61 tttgccgaaa caaacaattg gggatttttc actttcggac accacgccga gccttatgaa
      121 actgctgcca aagagagttc gattagccaa tgcgcctatt gccatatcgc gaatgttgca
      181 aaaacggata tggtttgggt tcaatattac cccctcctga aaacaaagat cgactgaacg
      241 actgaacttc cagttctctt tcgaactgct cttactcgac caaggctcag tgaacaaccg
      301 tattcgaatg aactcaacga ggtacgagca cgtaggtttt gccgcggttc acggtccagg
      361 tgcgaattca atacatctcc gagtaggatc tcctgattcg gcaggctttg ggggcaagga
      421 attgcctcaa aaagaccctt cagacaggaa ggctcgcgga agggagcaaa cggagtatgt
      481 gtgtatttcc cattcagaat tggttggtcc ctcccctgga gaacgaagat gattaccctc
      541 gcgaaataca aatactggat aggcggagca tttctcctgc tcgtgctcgg cgtcatcttt
      601 ggcgttgcgc tacccaagca caggtcggat gcgctcggtg ggccagcgga cgtccagatc
      661 gtgccggtcg agcagaagga cgtacagatc tatggcgagt ggatcggcac gctcgacggc
      721 ctcgtcaacg ccgacgtccg agcgcaggtg acgggttatt tgctgcggca ggattaccag
      781 gaaggcgcaa ttgtcaaaaa aggccagcta ctgttcgaaa ttgacccgcg gccttttcag
      841 gcatccttgg atcaagctga gggccaacta gctcaggcaa aggcgacgct ggtcaacatg
      901 gaggccgtgc aacgccggac acagctcgac gttgaccgtt acacgccgct ggccaaggag
      961 caggccgcca gccagcaaga cctcgacaat gcggtgcaga acaacctcgc ggccaaggct
     1021 aacgtcgaaa cagccaaggc tcagatcaag acttatgagg ccgccgtaga aaccgcacgg
     1081 attaatctcg aagttacgcg actcatcgcg ccgatagatg gcatcgctgg gcaagcccag
     1141 ctgcaagtgg gcgcgctcgt tagtcctgca agcggcgtgg tgacgtcggt ttccacggtt
     1201 agcccgatca aagtcttctt cacggctagc gagcctcagt atctcgaatg gcggagacgc
     1261 tttccgaccg agaccagtac acaagctgcc gctaaggctt tacatctcca attagtactg
     1321 gctaatggct cgacatatgc ccacgagggc accttctact tcgctgaccg ccaaatcaat
     1381 gagagcaccg gcgcgattcg gcttgccgcg ctgtttccca atcccgagag cattctacgt
     1441 cccgggggct atggccgagt tcgcgctgtg attcggaccc aacgggacgc actcgttgtc
     1501 ccgcagcgcg ccgtctcgga gctgcagggc ggctatcaag tagcggttgt ggatggcgag
     1561 aacaaagtga gcattcggcc agtcaaagtt agcgacaccg taggcagcca gtacgtgatt
     1621 gcggaaggcc taaagccagg cgaccgtgtg gtcgctgagg gcgtccagaa ggtgcgtccg
     1681 gcgatgaccg tgaaccccaa gccctttgta gcggagacaa agggggaata gttcgatgtc
     1741 gaagtttttc atcaatcgtc cgatcgttgc gatggtgatt tccatcctca tggtaattgt
     1801 gggtggaatc accatactcg ccctgccggt agctcaattc ccgaacatcg ctccacctga
     1861 agttcaaatc ctggccacct acgtcggcgc ggacgcccag acgcttgagc aggccgtggc
     1921 cacacccatc gagcagcaga tgaacggcgt tgacaacatg aactacatgt attcgctcaa
     1981 cgccacggcc aactccaaca cgaccctgat cgtaaacttc gacgttaaga cggacccgaa
     2041 tacggatttg atcctcgctc agtcccgcga aacgcaggcc gcctctcagc tccctgccga
     2101 cgtcaacaat tacggcatca ccgtccgaaa gtcagtaacc gcgcccctca tgttattggc
     2161 attgtactct ccgcatggga cgtatgatgc gaccttcctg tcgaactacg cgtacatcaa
     2221 tctcgtcgat ccaatcacgc gctcgtatgg tatcggcaat gttcaggtat tcggagcggg
     2281 ccaatacgcg atgcgacttt gggtaaaacc cgaccagctg gcaaagcttg gaatcaccgt
     2341 cacggacatc ataagcgcta ttcaagcgca aaacacagta aacccagcag ggcgggcagg
     2401 tggcgagccg gctcccaaag gacaggaatt tacttactcg gtcctcgctc agggtcggct
     2461 tatttctcca ggggaattcg gaaatatcgt tctccgcgaa actccagatg gcggagtcgt
     2521 tcgggtccgt gatgtcgcgc gaatcgaact gggttcccag gactacagca cgtccggccg
     2581 ctttaatggc aagccgtctg ccatcgttgc tgtataccaa ctgcccggtt cgaatgcagt
     2641 gaacgccgcc gacaacgtcc tgaagctgat gggtgaaatg aaacaccgct tcccagcgga
     2701 catggatttt gccacagctt tggacacaac gcgggcggtc acggaaggta tgaaagaaat
     2761 tgtcgtaacc ctgtttatcg ccctcgtcct cgtgatcctg gtggtttaca tcttcttgca
     2821 gggcttgcgc tcgacgctga ttccgctcct tgccgtgccg gtggcgctgg taggcacttt
     2881 catatttttc ccgctgttcg gattctccct caatacgctt tctctctttg gtttggtgct
     2941 cgcgattggt cttgtggtgg atgacgcgat tgtggtggtg gagggcgtgg aacggcacat
     3001 cgaagaaggc atgacgccaa agaacgcggc gttgaaagcc atggaggaac tctccggccc
     3061 agtagttggc atcgccctgg ttctgtcggc tgtattcgtg ccgacggctt ttattcccgg
     3121 catcaccggc cgcctgtacc agcagttcgc ggtcacaatc gcgatttctg tgctgctttc
     3181 cgctttcaat gcgcttacgc tcagccccgc gcttgcggcc ctcctgctcc gaccaaagga
     3241 gccaagccgc gggctcctgc ggagattctt tgactggttc aaccggactt tcgaacgagc
     3301 cacggagggc tatgtgcgtt ggagtgccgt gcttattcaa aagaccgtgg tcatggtcgt
     3361 cttgcttgta ggattttgca tcgcgggggg atttttcgcg aaccgcgttc cctcaagctt
     3421 tcttcctgat gaggatcaag gctatttcta cgttaatatg cagcttccca atagcgcttc
     3481 tttggaacgc accagcgacg tcgcggtgga tgtcgagaaa atcctggcca acactcccgg
     3541 cgtccggtac accaccagcg tcattggctt tagcttgctc agcttcgtac gcacaactta
     3601 caacgcgttc ttttttgtga ctcttaagcc gtgggacgag cgcacttcga gagccgagca
     3661 attgcaagcg atcaaggcgc atctcaatcg cgagctcagc aaagtgcctg cggcaattgc
     3721 cttcggtttt tctccgcccg ctattccggg tgtgggaact tcaggtggct tcacttttat
     3781 cctcgaagac cgttcgggca gcgacgttca gttccttgca aaaaatcttg ccactttcat
     3841 ggaagctgcg cgaaagcgac ccgaaatcgc ccaactcagc accactttcc tgcccagcgt
     3901 gcctcagaaa tttctcgatg tagaccgcga caaagtgctg aaacaaggag tcgtactgaa
     3961 cgacgtttac aagacgattc aggctttcat gggcggctac ttcgtcaact atttcaaccg
     4021 ctttggccga cagtggcaag tgtacatcga agccgagggc gaagaccgcg ccaaggcgga
     4081 aaacgtcggg cagttctacg tgcgcaacaa caagggcgag aacgtcccgc tttcctcact
     4141 ggtgacagtg aaaaatcgct ctggtcctga atttacgatg cgattcaacg agtaccgctc
     4201 cgcacagctc aacggcggct cggctccagg ctacagcgcg gagcaggcta cggcggcact
     4261 cgaggaagtt ttcaagcaga caatgccgcg ggaaatgggc ttcgactatt ccggcatgtc
     4321 gtttcaggaa cagaaagcgc gtgagggggt gcccgcctcg gtgatctttg gaatttccct
     4381 attgttcgtc tttctcattt tggcggctct atacgagagc tggtcgctgc cctttagcgt
     4441 gttgctcagc acgccagtgg cggtgtttgg cgcgtttgcg atcctttggt tgcgccgcgt
     4501 gctgctcggc tttttcgtgc ccccttacat ggtccagata gagaccgatg tttattcgca
     4561 gatcgggctc gtgatgctca tcggcttggc agccaaaaac gcgattctga tcgtcgagtt
     4621 cgccaaagac cagtttgaga agggcaggcc actggtggat gccgcgctcg aaggcgccaa
     4681 gcttcgcctc cgccccatcc tcatgacgtc cttcgccttc attctcggtt gcgtgccgct
     4741 gtggaccgca tctggcgccg gttccgtggc gcgacaaatt atgggcacca ccgtcatagg
     4801 cggaatgctc gccgccagct cgatcggcat cttcttcgtc ccggcaattt tttacctcgt
     4861 cgaaaaatgg tctggtgcgg gtaaagaacc ggctttagga acgcttcccg ctgcgcctgc
     4921 agcccacccg cagccgggcg attgaaaaat tcattgaaga atccagattg atgctcgaaa
     4981 tcgaccacac gcggagttgc ttctcatgaa gctcgctaaa tccctaattc ctgtcgtcgc
     5041 ttgcgcttgg ctcgtcggct gcaaagttgg tcccaattat cgtcgccccg tcgtccagac
     5101 tccaaccgca taccgcgacc tctctgaaaa tcctcagctt caggcccagg ccgcgtctta
     5161 tgcggatctt ccgtggtggc aagtgttcca ggacccacag ctgcaggact tgattcgcac
     5221 ggcgctcaag caaaattacg acctccagct tgcgaccgaa cgtattaatg ccgcccgcgc
     5281 ccaggttgcc atcacgcgct ccagcctctt cccgcaagtc gcggcgggcg gcactttcac
     5341 cggcggaaaa gaaagcaact ttcaaaccag gtttaacttc ctcacgctgg ccgccgacgc
     5401 cacgtttcag ttggacttct tcggcagact gcgccgcgcc accgaagccg cgcgtgccga
     5461 gctgctggcc acacaggctg cgcggcaaac tgtgatactg acattggtga gcgatgtggc
     5521 cagcgactac ttttcgctgc tgcaactcga tttgcagctc caaatcacgc ataacaccat
     5581 caatgcgcaa acagaatccg tgaagctcac caagtttcgc gtagatcacg gcgtggcgac
     5641 aaaacttgat gtgcttcaag cgcagcaaac actcgatacg gccaacgcaa cggttcctga
     5701 cttggaacgg cagatcgcac aggaagagga cgccatcagc attttgctag gagattatcc
     5761 gcacgctgtt actcgcgggc ttcccttggt cgatcagctg cttcccccgg cggtaccccc
     5821 agggttgccg tcgtcgctgc tcgagcgtcg gcccgacatt cgcgaggccg agcaaaatct
     5881 cattgccgca aacgcggaaa ttggcgtcgc gaaggcccag ttcttcccgc aaatcgaact
     5941 gaccggttcc ggcggcggtg ccttcggtcg cagctccacc ttctcaagcc tcatgccctc
     6001 gcaacttggt atttggtcct acggcgccca ggtcactcag ccgatctata ctggcggcgc
     6061 cctaaagggc aatttgcact tggccgagtc gcaataccgg caactcttca tcgcctacct
     6121 ccagacgatt caacaggcct tcggggacgt ctctgattcg ctcatcggct atgaaaaact
     6181 ccatcaggtc cgtgtgcggc aggaggaaac tgtggcggac ttgcaggagg ccgtccgcct
     6241 ctcgaacatg cggtacaagg gaggcaccac gacgttcctt gaagtgctcg acggccagag
     6301 ggcgctcttt tccgctgagc tgacgctagc gcaggcacga ggcagcgaat accaaagcct
     6361 cgtgcagctc tatcgcgccc tcggcggcgg ctggcagcag tgaaaaaccc acgtcgatac
     6421 acaccggcat ctaagactct aacgacccga ccaatgggct catctacttc ggtgcaaacg
     6481 acccctgttc ggcactactt gcaaccaaaa ctggtcagtc aaaaattgat tgctctccga
     6541 ccttggtcgc gaaatgcatc agcgattgac agcgctgtcg ccgtgctgat gtttctgacc
     6601 acgctttcgt tcttgttttc tacgccggga tgggagccga cgcgcggtgg ttttcctgcc
     6661 ctttcgggag cagtaggcca attcttatta aaagatctgg tcctgcaggg ttttcgcaat
     6721 ttggtccctc ggtgaagcct tggccggaca acgcaacggc gcgtgagcgt gaattgctgc
     6781 aggcccatcc acgaatgatt gccgcctgag cccgacagaa tgatagccgt caactgccat
     6841 tttcgaggct ctatgaagga agccatggaa aagaagccaa cagacgaatc tggggtgcca
     6901 ggctgcccga acagatcgga cggcgggagt gagagccgtc ggtttcactg gccggaatac
     6961 ctgatggaag cgggcgagct aggcctgttt atgttttctg tttgtgtgct cgctacatta
     7021 tttagacatc cgtcttcgcc gctttcgcac ctggtggttg gcgagctggc gcgcagactg
     7081 ttcattggat tggcgaccgg cgccatggtc gtcatgattg tgctaacgcc ttggggcaaa
     7141 cagtccggtg gacatttcaa tccggcgatc actttcacgt tctatcgcct cggcaaggta
     7201 gatgcctggg atgccatttt ctacggcgtc gcgcaattcg caggcgcagt ttccggcgtt
     7261 atgttggcgg tatatctcct ccgcggtgca ccgtcccatg atcaggtacg ttacgcgatg
     7321 actgtgcccg gtaggttcgg cactgttgcc gccttcctcg cggagtttgc gatttccttc
     7381 atgttgatga cgacgatcct gtacgcgacg aatcacgcag catatgctcg attcactcct
     7441 tatctcgtag gtgcgcaata tgcgctgtac atcacgttcg aaacgccttt gtccggcatg
     7501 agcatgaacc ccgcgcgaac gttcgggccc gcttttcatg cgtcttactg gcacgcgctc
     7561 tggatttact tcgttgcacc gacgctcggc atgctagtct cagcggaagt gtttctgcac
     7621 tgtcgcgatg gccttcgtcc gtactgcgca aagctatacc atgcccccga caagggctgc
     7681 atatttcgcc acgacgggcc aacggttgcc tagacgtttc gccgcaagcg gcggcgatca
     7741 catggcgtgc ctcagcgcgc agaattagcc tatctgccca ttgctcggaa ttgaacaccc
     7801 tcagcccgca ggagggcaaa caccagaagg agagaacact tgctgtggaa gcaggttcgt
     7861 cttgaaaaac ccctgagagc acctgcctgg atagcaggtg ctcggccgag atcgccacaa
     7921 atgatatcga tcttagctgt ttgcgttgag gacgaacgtc gtttagtgtt taatgttttc
     7981 atggagattt catgaacata ttagccgcac tgaagcagga agaagcgaag ttg
//
LOCUS       DASTPM010000452         8416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1024972, whole genome shotgun sequence.
ACCESSION   DASTPM010000452 DASTPM010000000
VERSION     DASTPM010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024972"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFR42_07985"
     CDS             complement(<1..642)
                     /locus_tag="VFR42_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU0369132.1"
                     /translation="MQTGIIGLPQVGKTTLFRILTKAHIDPAKGGQETRVGVAKVPEP
                     RLAQLATLYNPKKITYATVQYVDLGGMQKERMRESLASLREVDAIAHVIRVFDDPSVP
                     HAEGTIDPLRDATNLELELILSDLEQIEKRLERLEKDLKKKADKNFELEKTVLEKCKA
                     HLHAEQPLRELPLTAEEKKPIGGFLFLSQRPMLFVLNLGDEEASQLESAVERHK"
     gene            804..1553
                     /locus_tag="VFR42_07990"
     CDS             804..1553
                     /locus_tag="VFR42_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369133.1"
                     /translation="MANTALPFDLAAATAHLSATDPCLAKLIAELIPFEPEIDHDQSP
                     YEALMEAIAYQSISGKAAATIFARIKALSANGGVPTPEEVLKLNTRQLRKAGLSGAKI
                     LSMKDLAQKTIDGIVPTHDEALKLSDEELVERLDSVRGIGAWTVEMFLIFRLGRPDVL
                     PIHDLGVQKGWSVAYGKKHKPRPKELLKFGERWRPYRTVASWYMWRAFERAGYAATNK
                     IKKSRVKRKVRIKIKQAKVVVKKRAFRGRNK"
     gene            complement(1630..2106)
                     /locus_tag="VFR42_07995"
     CDS             complement(1630..2106)
                     /locus_tag="VFR42_07995"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEU0369134.1"
                     /translation="MAFFRAVEFLGLSIWLGSDVFLSFVVAPGAFSVLAGRDQAGAIV
                     GYSLTRMHWMGVICGVLLLLLRVARTRTVASLAAPAALCVLLMILLTVTSQLAVSPKM
                     AALRVQMGSIQAAAADNPLLAEFSRLHRVSVALESGVLLAGFAALYLLVRETALNA"
     gene            complement(2148..2297)
                     /locus_tag="VFR42_08000"
     CDS             complement(2148..2297)
                     /locus_tag="VFR42_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369135.1"
                     /translation="MGQQYWDFQLKNLLLDPWGMISLVLTPIYLVTNTVSSATARREL
                     AGAVE"
     gene            complement(2305..2478)
                     /locus_tag="VFR42_08005"
     CDS             complement(2305..2478)
                     /locus_tag="VFR42_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369136.1"
                     /translation="MITANPTPATAIPNNNLNAIGRCQLCGSTRLTAHVKFERNIGML
                     VLRQTRRAASPPL"
     gene            complement(2666..2741)
                     /locus_tag="VFR42_08010"
     tRNA            complement(2666..2741)
                     /locus_tag="VFR42_08010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2706..2708),aa:Val,seq:cac)
     gene            2764..3768
                     /locus_tag="VFR42_08015"
     CDS             2764..3768
                     /locus_tag="VFR42_08015"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0369137.1"
                     /translation="MRETQRRLSFVAGLTGVFNMPTATYQVVSQLQRSIARTIYGKEE
                     AIQLALITLLARGHLLIEDVPGVGKTTLAQALAKSFHCSFQRVQFTSDLLPSDVLGVS
                     VYQPNTHEFEFRQGPLFANVVLADEINRTTPRTQSALLEAMNEAQVTVDGKTYPLPQP
                     FLVIATQNPIEHHGTYPLPESQLDRFLMRIKMGYPSPETEREILRRRAFDNPMEKLEP
                     VADVNEVLAMPEAVTHIKVDASLHDYALDIVNRTRTSDQLALGVSPRGTLMLQRAAQA
                     RAFLDGRDYCVPDDFKQLAVSVFAHRVVASARHASLQRKSETTEAALREIVESVRVPV
                     "
     gene            3864..5219
                     /locus_tag="VFR42_08020"
     CDS             3864..5219
                     /locus_tag="VFR42_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU0369138.1"
                     /translation="MSQQTSLLTQLWAKAGTPGVRAFFLSILALGVALLLAVYSGAAA
                     ELGNLGLAASSALLALAIAGWVAVTIVPTLAKRTPLRWIGYRMEYKISRAGWIYIGAT
                     LLVALAALNTGNNLLFLILACLISVILMSGILSSISLAGVELRIELPEHIFAGQTVRA
                     TVELLNEKLMLPSFALRVEAAVAKDAPAAAVLETPVFFPFLPRQERVKQSVPVTFLRR
                     GLHVQDAFRIVTRFPFGFLQKTRRVSLKSEALVYPSVETSSELADMFPGIEGSLESHS
                     KGRGQDLHALREYLPTDSARHVHWKASARRGALMVREFARDDDRRVLLVLDPYSGGAE
                     RVGGAVEEERFEYAVNLCAGIAWHFFERNAQLEFRGAGIETRLAPAEEIIFAILGYLA
                     VAQPEKPGAESPLLQELAASPELFKLIVTSRQRGTIPSDLWHSSYVIFLESLEERRGR
                     S"
     gene            5226..6407
                     /gene="mutY"
                     /locus_tag="VFR42_08025"
     CDS             5226..6407
                     /gene="mutY"
                     /locus_tag="VFR42_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU0369139.1"
                     /translation="MNKVIRSLPPNAIHSLSLEGQLKEFRCALLAWFAQFQRDLPWRR
                     SRDPYRIWISEIMLQQTRVAAVIPYYERFLARFPNVQALAAAPEEEVLRVWSGLGYYS
                     RARNLQQAAKQIVAKHGGEFPRTKAEALELAGIGGYTAAAILSIALGEKLAVLDGNVA
                     RVLARLDVLRGDLRSPGRWRELQKRADELLDCEAPGDWNQAMMELGATLCAPRAPQCL
                     LCPVQSFCNAHKLGLADEIPLRRAKRAAVGIRLAAVVLMDSKGKSLLLPPPTAANGNK
                     ADDHVPSLVAKLWHFPTVSTAGNMETELKRFLKQEFALRDSTNLVLERLTKVRHTVTY
                     RKIELAPFLIRMKSLPRISGAKAMRLDAILREPISNLTRKVAQAAIKKLASQQKTDQR
                     S"
     gene            6466..>8416
                     /locus_tag="VFR42_08030"
     CDS             6466..>8416
                     /locus_tag="VFR42_08030"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEU0369140.1"
                     /translation="MPSRTSLPVVPPLPAERFFRVSLFLLLFTAILTLIGTGKIDIFT
                     SAVAMVALLCHVRRWWYGHEPELKPRTATFLVLCYLLFFPFDMFFLSRSLAANSPNPP
                     LYAALISSVHFLLFILLVRLYSARTDRDAHFLVMLAFAALLASAVLTVDTAFLFLFFF
                     FLLFAIATYTGLELRRGAAGAALPTTSAQPESEKRLARALGYSALGVCLGAIVCGTVL
                     FFVFPRVSAGYVGKTSFNPTLLSGFTDQLELGQIGEIKKSEAVVMRVETGKPVNYPGL
                     RWRGNALANFDGRRWSVGERGTETLLANAEGWIPLRERPRAGEIRGEILQFIVLQEPM
                     ASDLLFVPGDALALRGNFTGEAGGISHRRAYIYRDAAGSLSNPFHNYVAIRYTGLSQL
                     PPLDKARLEAAGTDYPPDISEKYLQLPLELDARIPALAISAASRGVTPYDKAAVLENF
                     LKTKYAYTLNLTGNPGKDPLAHFLFETRAGHCEYFASAMTVMLRTMGIPAREVNGFLP
                     GEYNDLGGDYIVRASDAHSWVEAYFPGNGWIVFDPTPAAPAVSMTLFKRISQFADWAE
                     LTWNDWVIGYDFAHQTALAQTVQMRSRNWREVAASWFSRKQQNFRSRLSGWQLQHQAL
                     SFALPVLLIGLLIALRYGRIADLLAR"
BASE COUNT         1748 a         2212 c         2663 g         1793 t
ORIGIN      
        1 cttgtggcgc tcgacggcgg attcgagctg gctggcttct tcgtcgccca ggttcagaac
       61 aaagagcatc ggccgctgtg agagaaacaa aaatccgccg atgggctttt tctcttccgc
      121 ggttaacggc agttcgcgca agggctgttc ggcgtgcaag tgcgctttgc atttttccag
      181 gacggtcttc tcgagttcaa aattcttgtc ggccttcttc ttcaggtcct tttccaagcg
      241 ttcgaggcgc ttctcgatct gctcgaggtc ggaaagaatg agctctaatt ccaggttggt
      301 agcatcgcgc aacggatcga tggtgccttc cgcgtggggc acgctcgggt catcgaaaac
      361 acggatgacg tgagcgatgg cgtcgacttc gcggagggat gcaagagatt cgcgcatacg
      421 ctccttttgc atgccgccga gatcaacgta ttgtaccgtc gcgtaggtga ttttcttggg
      481 gttatagaga gtagcgagct gggcgaggcg cggttcgggc accttggcca cgccgaccct
      541 cgtttcctgg ccgccttttg cgggatcgat gtgcgcttta gtcaaaatcc gaaagagcgt
      601 ggtcttgccg acctgcggta gtccgataat tcctgtttgc attccgtcgc agtcctaatc
      661 tagcggagaa ttttggattt gtgtaaacgg gaaattttag cacaccggct atcaggctgt
      721 ggcgcgcctc cggggctgaa gagaaagcgg aagctgggct gctgcaatcc aaaaaggaag
      781 gggttgcgct atgatttttg gctatggcga ataccgcact tccgtttgat ttggcggccg
      841 cgacagcgca tttgtcggcg accgatccgt gtttggcgaa gctgattgcg gaattgattc
      901 cgtttgagcc ggagattgat cacgaccaat cgccgtatga ggcgctgatg gaggcgatcg
      961 cgtatcagtc catttcgggg aaggcggcgg caacaatttt tgcgcggatc aaggcgttaa
     1021 gcgcgaacgg cggtgtgcct acaccggaag aggtgctgaa gctgaacacg cggcaattgc
     1081 gcaaagccgg gttatcgggc gcgaagatcc tatcgatgaa agacctggca cagaagacga
     1141 ttgacggcat tgtgccgacg catgacgagg cactgaaact ttcggatgaa gaattggtgg
     1201 agcggctgga ttcggtgcgt gggattggcg cgtggaccgt ggagatgttt ttgattttcc
     1261 ggctgggacg gcccgacgtg ctgccgattc acgatctggg agtgcaaaag ggctggtcgg
     1321 tggcatacgg aaaaaagcac aagccgcggc cgaaggagct gctgaagttc ggagaacggt
     1381 ggcggcctta ccgcacggtg gcgtcgtggt acatgtggcg ggcgtttgag cgggcggggt
     1441 atgcggcgac gaacaagatc aagaagtcgc gggtgaagag aaaagtgcgg atcaagatta
     1501 agcaggcgaa ggttgtggtg aaaaaacggg ctttccgagg ccgcaacaaa tgaatgagag
     1561 attcctcgct tcgctcggaa tgacgtgggc cggtaaggtg gaatgcaggt tcggcggtac
     1621 atgatgaagc taagcgttga gagctgtttc acgcacgagt aggtagaggg cggcgaaacc
     1681 ggcgaggagg acgccgcttt cgagtgcgac ggaaacgcgg tgaaggcggg agaattcggc
     1741 gaggagcggg ttatcagctg cggcggcttg aatcgagccc atttgaacac gcagcgcggc
     1801 cattttgggg ctgacggcca gctgcgaagt gacggtcagc aaaatcatga gcaaaacgca
     1861 aagcgcggcc ggggcggcaa gactggctac ggtacgcgtt cgtgccacac ggagcagcaa
     1921 cagcaaaacg ccgcagatga cgcccatcca atgcatgcgc gtgagcgaat agccgacaat
     1981 ggctccggcc tggtcgcggc cggcgaggac agaaaaggct cccggagcga cgacgaagct
     2041 gagaaagacg tcgctgccga gccagatgct gagccccaag aattcaactg cgcgaaaaaa
     2101 ggccataggg taactcgcgc gatcaggacg agagagtcca gtagatttta ctctaccgcg
     2161 ccagcgagtt cgcgacgagc ggtggcagag ctgaccgtat tcgtgaccag ataaatcggc
     2221 gtgaggacca gagagatcat gccccacggg tcgagcagaa ggtttttgag ctggaagtcc
     2281 cagtattgtt ggcccacgca ggtcctacag aggcgggctt gcagcccgcc gcgtctggcg
     2341 caggacgagc attccgatat tgcgttcaaa cttcacatgg gcggttaggc gcgtgctgcc
     2401 acagagctgg caacggccga tcgcgttcag attgttgttg gggattgcag tcgcgggcgt
     2461 ggggttcgca gtgatcatgt ggacctccgt ttgaactcgt tcaagcgaga ttgtccgtgc
     2521 gaaaaagcgc gagagaacgt tcgttcgatt caagctagca tggaagtgaa agaaatgacc
     2581 tgtaccatac gcgatgaata accatcgttt ggacacaaga cagacggcca actttcggca
     2641 caacacaatc aatggcaaga tgaagtggtg ggcgataaag gatttgaacc tttgacctct
     2701 cgcgtgtgag gcgagcgctc taaccgctga gctaatcgcc cgttttgaaa cgggtagagc
     2761 gacttgcgag aaacgcaacg ccgcctttca tttgtagcag gattgaccgg ggttttcaac
     2821 atgccgaccg caacctatca ggtagtttcg cagctgcagc gctcgatagc gcggacgatt
     2881 tacggcaaag aagaagcgat tcaactggcg ctgatcacgc tgctggcgcg cgggcatttg
     2941 ctgatcgagg atgtgccggg agtggggaaa acgacgctgg cgcaggcact ggcgaaatcg
     3001 tttcattgca gctttcagcg cgtgcagttc acctcggatt tgctgccgag cgatgtgctg
     3061 ggcgtgagtg tataccagcc gaatacgcac gaatttgagt ttcgccaggg cccgctgttt
     3121 gcaaacgtcg tgctggcgga cgaaatcaat cggacgacgc cacggacgca atcggccctg
     3181 ctggaagcga tgaacgaagc gcaggtgacc gtggacggca agacgtatcc gctgccgcaa
     3241 ccgttcttgg tgatcgctac gcagaatccg atcgagcatc acgggacgta tccgctgccg
     3301 gaatcacagc tggaccgatt tttgatgcgg atcaagatgg gctacccgtc gccggagacg
     3361 gaacgggaga ttttgcgtcg gcgggcgttc gacaatccca tggaaaagct ggagccggtg
     3421 gccgacgtca acgaagtgct ggccatgccg gaagcggtga cgcacatcaa ggtggacgcg
     3481 agcctgcatg actatgcgtt ggacattgtg aatcgcacgc gcacttccga ccagctggcg
     3541 ctgggagtaa gtccgcgagg tacgctgatg ctgcaacgcg cggcgcaggc gcgggcgttt
     3601 ctggacgggc gcgattattg cgtgccggat gacttcaaac aattagcggt ttccgtgttt
     3661 gcgcatcgcg tggtggccag cgcgcggcac gcttcgctgc agagaaaatc agagacgacg
     3721 gaagcggcac tgcgggaaat tgtcgagtcg gtgcgcgtgc cagtgtaagc gaaccgatac
     3781 ggcgtcacaa aggacgcggc attaagcgct gctaaatgga acggctggcc gcaagtcgac
     3841 aggttagcgt ttctgaagaa ttcatgagtc agcagacttc cctgcttacg cagctatggg
     3901 ccaaggcggg cacgccgggc gtgcgcgcgt tttttctttc cattttggcg ttgggagttg
     3961 ctctgctgct cgccgtgtat tccggcgctg cggcggagct tggcaatctc ggcctggcgg
     4021 caagttcggc gcttctggcg ctagcaatcg ccgggtgggt tgcggtgacg atagtcccga
     4081 cgctggcgaa gcggacaccg ctgcgctgga tcggttatcg aatggagtac aaaatcagcc
     4141 gcgcgggctg gatttacatc ggcgcgacgc tgctggtcgc cctcgccgca ctgaataccg
     4201 gcaacaattt gctgttcctg atcctggcgt gcttgatttc cgtaattctg atgtcgggca
     4261 ttctttcttc catttcgctg gcgggcgtcg agttgcgcat cgaactgccg gaacatattt
     4321 tcgcggggca gacggtgcgc gcgacggtag aactgctcaa cgaaaaatta atgctcccat
     4381 cgttcgcgtt gcgtgttgaa gctgcagtgg cgaaagacgc tccggccgcg gcggtcctcg
     4441 agactccggt gttctttccg tttttaccgc gacaagagcg agtaaagcaa agtgtgccag
     4501 tcacgttttt gcggcgcggg ctgcacgtgc aggatgcttt ccgcattgtg acgaggtttc
     4561 cctttggatt cttgcaaaag acccggcgcg tgtcgctgaa gtccgaggcg ctggtctacc
     4621 cttcggtgga aacaagttcg gagctggctg atatgttccc cggtattgaa ggcagcctgg
     4681 aaagccacag caaggggcgc ggacaggatc tgcacgcact gcgcgaatat ttgccgaccg
     4741 acagcgcgcg gcacgtgcac tggaaagcct cagcacgaag gggcgcgctc atggtgcggg
     4801 aattcgcgcg cgacgacgac cggcgggtgc tactcgtgct tgatccctat tccggcggcg
     4861 cggaaagagt cggaggcgcg gtcgaggaag aacgcttcga atacgctgtg aatttgtgcg
     4921 cgggaattgc gtggcatttt ttcgagcgca atgcgcagct ggaatttcgc ggagcgggaa
     4981 tcgagactcg cctggcgccg gctgaagaaa tcatttttgc aattttgggt tatttggcgg
     5041 tggcgcaacc ggaaaagccg ggcgcggaaa gtcctttgtt gcaggagctt gcggcgtcgc
     5101 cggagctctt caaattgatt gtgaccagcc ggcagcgcgg caccattccc tccgatttgt
     5161 ggcactcttc atatgtaatc tttctggagt cgcttgagga acgccgcggc cgctcctagg
     5221 tgcgcatgaa caaggtcatc cgctcgcttc cgcctaacgc catccactcg ctttcgctcg
     5281 aaggacaatt aaaagagttt cgctgcgcgc tgctggcatg gttcgcgcag ttccagcgtg
     5341 acttgccctg gcggcgttcg cgggaccctt atcgcatctg gatttcagaa atcatgctgc
     5401 agcagacgcg cgtagcggca gtaattccgt attacgaaag atttctggcg cggtttccga
     5461 acgtacaggc attggcggcg gcgccggaag aagaggtgct gcgggtgtgg tccgggcttg
     5521 gctactacag ccgggcgcga aatctccagc aggctgcgaa gcagattgtg gcgaagcatg
     5581 gcggcgaatt cccgcggacg aaggcggaag ctttggaact ggccggaatc ggtgggtaca
     5641 cggcggcagc gatcctcagc atcgcactcg gagagaaact ggctgtgctt gacggaaatg
     5701 tcgcgcgagt gctggcgcgg ctggacgtac tgcgcggcga cttgagaagc ccgggccggt
     5761 ggcgcgagct gcagaagcga gcggacgaat tgttagattg cgaagcgccc ggcgattgga
     5821 atcaagcgat gatggagctg ggcgcgactt tgtgtgcgcc gcgagcgccg cagtgtttgc
     5881 tgtgccccgt acagagcttt tgcaacgcgc ataaacttgg ccttgccgat gagattccgc
     5941 taagacgagc gaaacgcgcc gcggtgggga tccggttagc ggcggtggtt ctcatggaca
     6001 gcaaaggcaa gtcgttgctg ctgcctcctc caacagcggc aaacggaaat aaggccgatg
     6061 accatgtacc gtcgctggtc gcgaagctgt ggcactttcc cacggtatcc acggcgggaa
     6121 atatggaaac ggagctgaaa cgattcttga agcaagaatt tgcactacgg gattcaacga
     6181 atttggtact tgagcggcta accaaggttc ggcatacggt gacatatcgt aagatcgaac
     6241 tcgcgccttt tctgattcga atgaaaagcc tgccacgaat ttcaggtgca aaagcaatga
     6301 gattggacgc aatcctgcgg gaaccaatct ccaacctcac ccgcaaagtg gcacaagccg
     6361 caatcaagaa gctagcctcc caacaaaaaa ccgaccagcg aagttgattg attggacctg
     6421 ctcggccgcg ccgcgcagtc cgaatgatag gatggaggtt cagccatgcc ctcccgtact
     6481 tccttgccgg tcgttccgcc gcttcccgcc gaacgatttt ttcgcgtctc attgtttctg
     6541 cttctcttca ccgcgatctt gaccctgatc ggaaccggca aaatcgacat cttcacatca
     6601 gccgtggcca tggtggcctt gttatgccac gtgcgccgct ggtggtacgg gcatgaacca
     6661 gagctgaaac caagaaccgc gacatttctt gttctttgct acttgctatt ctttcccttc
     6721 gacatgtttt tcctgtcgcg cagtctggcc gcgaattcgc cgaatcctcc gttgtacgca
     6781 gcgttgatca gttctgtgca tttcttgctc ttcatcctgc tggtcaggct ttatagtgcg
     6841 cgaaccgatc gcgatgcaca ttttttggtg atgttggcct ttgcggcttt gctggcctcc
     6901 gccgtgctca cggtagatac ggcattcctg tttctttttt tcttcttctt gctttttgcg
     6961 attgctactt acaccggcct ggaattgcgc cggggcgccg cgggcgccgc attgcccacg
     7021 acatcggctc aaccggaaag cgagaagcgg ctggcgcgtg cgctgggtta ttcggcgctg
     7081 ggcgtctgcc tgggcgccat cgtttgcgga acggtactgt tcttcgtctt tccccgtgtc
     7141 agcgccggct atgtgggtaa gaccagtttc aatccgacgt tgctcagcgg gttcactgac
     7201 cagctggaac ttgggcaaat cggcgagatc aaaaaaagcg aagccgttgt gatgcgcgtg
     7261 gaaacgggca aacccgtcaa ttatcccggc cttcgctggc gagggaatgc gttggccaat
     7321 ttcgatggcc ggcgctggtc ggtcggcgaa cgcggcacgg aaactttgct ggcaaatgcg
     7381 gagggatgga ttccgctgcg cgagaggccg cgcgccggcg aaatccgcgg cgagattctg
     7441 caattcattg ttttgcaaga accgatggct tccgatttgc tgttcgttcc gggcgacgct
     7501 ctggcgctgc gcggaaactt cactggagag gctggcggca tcagccaccg ccgggcctac
     7561 atctaccggg acgcagcggg ctctctttcg aacccatttc ataactatgt cgcgattcgc
     7621 tataccggcc tttcgcagct gcctcctctg gacaaagcca ggttggaagc tgcgggaacg
     7681 gactacccac ccgacatcag cgaaaagtat ctgcagcttc cgctcgaact ggacgcgcgc
     7741 attcccgcgc tggccatctc cgcagcaagc cgcggcgtca ctccttatga caaagcggcg
     7801 gtgctggaaa acttcctaaa gacgaagtac gcatacacgc tgaacctgac gggaaatcca
     7861 ggtaaagacc cgctggctca ttttcttttc gaaacgcgag ccgggcactg cgaatatttt
     7921 gcgtcggcga tgacagtgat gctgcgcacg atgggaattc cagcgcgcga agtgaacgga
     7981 tttttaccgg gtgagtacaa cgatctcggc ggcgattaca ttgtgcgcgc cagcgatgcc
     8041 cacagttggg tagaagcgta tttccccggc aacggctgga tcgtttttga tccgaccccg
     8101 gcagcgccag cggtctcaat gactttgttc aagcgcattt cacaatttgc ggattgggcg
     8161 gagctgacct ggaacgactg ggtcatcggc tacgacttcg cgcaccagac ggcgctggca
     8221 cagaccgtgc aaatgcgatc gcgcaactgg cgtgaggtag cggcttcatg gttcagccgg
     8281 aaacagcaaa acttcaggag ccgactgagc ggatggcaat tgcagcacca ggcccttagc
     8341 tttgcgctgc ccgttcttct aatcggtctc ttgatagcac tacgctacgg acggatcgca
     8401 gatttgttgg cgcggc
//
LOCUS       DASTPM010000453         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1399940, whole genome shotgun sequence.
ACCESSION   DASTPM010000453 DASTPM010000000
VERSION     DASTPM010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399940"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFR42_08035"
     CDS             <1..453
                     /locus_tag="VFR42_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0369141.1"
                     /translation="VPDLATRDGEWIFGVPLVLRFVERMVFPGADPGTVLLHPVARAA
                     WVGLLATSLNLLPAGQLDGGHILRSLNVRVHKWIGLAVPVVLIALGFVNRSAKVWLLW
                     AGILLALRFLRIPPVYDERPLDPIRVALAAASLLVMILCFMPVPLWEP"
     gene            535..1896
                     /locus_tag="VFR42_08040"
     CDS             535..1896
                     /locus_tag="VFR42_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0369142.1"
                     /translation="MASLVRTLRLRDILLLFIGSVIGSGIFLTPGLILKQVHGSVGLA
                     MLVWVAGGVMSLLGALTYAELAAANPEAGGLYCFIRDGFGRLPAFLYGWCLFLVISSA
                     TIAALARAFSRYLAEVIPLSTFQSNLAAMAMIAVVAVVNVRGTRHSSDLQNWTTLIKG
                     GLVVMLSVVLLALGKHAGEIPASAGTSLSGVELFSSFSLAMIAVLWAYEGWQFGTYSA
                     GEVVEPQKVFPRAFLLGSLLLMGLYVVAVSGYLVALGPARASESEAIAATAVNAVLGP
                     WAGKIVALTILISVFSATNSVALTAPRVFYAMAKDNLFFKTLAEVHPRFHTPAAAIIA
                     LCAWSAALTFAGKFSELIDGVVFIGWIFYGVSGAAIFPLRRRGAGKQQPYRVPGYPWT
                     PMVFLIGAAAVVGNAIYLATKDPQQFRHVLAALFLMALGVPAYFFWNLRNADTASDTT
                     LPR"
     gene            complement(1885..2205)
                     /locus_tag="VFR42_08045"
     CDS             complement(1885..2205)
                     /locus_tag="VFR42_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369143.1"
                     /translation="MFIAATAQAQWKSPRILAPLLGLGKHSYEVYLTHMFVVFAFFDL
                     FVYLGKPLRLAPILFTVVILMAGFLGLLIATTYSEPVNRFLRSRYGNKMLSKCAKSEK
                     ESSR"
     gene            complement(2239..3069)
                     /locus_tag="VFR42_08050"
     CDS             complement(2239..3069)
                     /locus_tag="VFR42_08050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU0369144.1"
                     /translation="MASADSTNQTAAWKRLDGIDLLRGLAIFFVLMNHINIRLLGAKV
                     PYLKFLPEQLVHFLVWNGQLGVQMFFVVSGFLITSMSLRRSDALTRISVRDFYLLRFA
                     RIGPLLLLVLAVLTALHFAKIEDFVVPAKTGGLGRALLAALTFHVNVLEARQGYLPAN
                     WDILWSLSVEEMFYLFFPLVCWLSGSRRFLVPILLIFVVLGPFSRTVFAHGNEVWEEY
                     SYLGGMEAIALGCLTALLASQVRFSVRSLWVVGACGTATVSLSLIFSWQAYLGWPART
                     "
     gene            complement(3186..>3438)
                     /locus_tag="VFR42_08055"
     CDS             complement(3186..>3438)
                     /locus_tag="VFR42_08055"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369145.1"
                     /translation="KAIQWSKQLSQFLAPHAGNEIRVLGPASAPLSRLKSEYRFQFLL
                     KSRKKAALAKLLAAALSYADSKEIPQTALLVDMDPLQLL"
BASE COUNT          704 a          815 c         1144 g          775 t
ORIGIN      
        1 gtgccggact tggcgacacg agatggagag tggatctttg gcgtgccgct ggtgttgcgg
       61 ttcgtggagc gaatggtctt tccaggtgct gacccgggca cagttttgct gcatccggtg
      121 gcgagggctg catgggtggg attgttggca acttcgttga atttgttgcc ggcggggcag
      181 ctcgacggcg gacacatctt gcgatcgctg aacgtgcggg tgcataagtg gatcggcttg
      241 gctgtgccgg tggtgttgat cgcgttggga tttgtgaacc gctccgcaaa ggtgtggctg
      301 ttatgggcgg gaatcttatt ggcgctgcgg tttttgcgaa tcccgcctgt ctatgatgag
      361 cgaccgttgg atccgatacg cgtagctctg gccgcagcgt cgttgctcgt gatgattttg
      421 tgtttcatgc cggttccttt gtgggagccg tgaagttttc gttgacgcga gtcggctgac
      481 gactatgatg cttgcggcgc gcgcctttct gttgcacgca tccagcagga cttgatggcg
      541 tcactcgttc gcacacttcg gctccgggac atcttgttgt tgttcattgg ctccgtcatt
      601 ggctcgggaa tttttctgac gccgggattg atcctgaaac aagtacatgg ttcggttggg
      661 ctcgcgatgc tggtgtgggt ggccggtgga gtgatgtctc tgctgggcgc actgacgtac
      721 gcggagctgg cggctgcgaa tcctgaggcc ggtggtttgt actgttttat tcgcgatgga
      781 tttggaaggc tgccagcgtt cttgtacggg tggtgcttgt ttctggtcat ttcgagcgcg
      841 acgattgctg cgctggcacg agcattttcg cggtatctcg ctgaggtaat ccccttaagc
      901 acatttcaat caaatttagc agcgatggcg atgatcgcgg tggtagccgt cgtgaatgtg
      961 cgcgggacgc ggcacagttc ggatttgcag aactggacga cgctgatcaa gggcggactg
     1021 gtggtgatgc tgagtgtggt gctgctggcg ttgggtaaac atgctggtga aattcccgcg
     1081 tctgcgggga cttcgctgag cggagtagag ctgttttcga gtttcagcct ggcgatgatt
     1141 gcggtgctat gggcgtacga agggtggcag ttcgggacgt acagcgcagg agaagttgta
     1201 gagccgcaga aagtttttcc gcgcgcgttt ttgctgggtt cgctgctgtt gatgggattg
     1261 tatgtggtag cggtgtcggg gtatctggtt gcgctgggtc cggcgcgcgc cagcgaaagc
     1321 gaagcaattg cggcgacggc ggtaaatgcg gtgctggggc cgtgggcggg gaaaatcgtg
     1381 gcgctgacga ttttgatttc ggtgttcagc gcgacgaaca gcgtggcgct gacggcgcct
     1441 cgggtgtttt acgcgatggc gaaggacaat ctgtttttca agacattggc ggaagtgcac
     1501 ccgagatttc ataccccggc cgcggcgatt atcgcattgt gcgcgtggtc ggcggcgttg
     1561 acgttcgcgg gaaagttttc tgaactgatc gacggtgtcg ttttcatcgg ctggattttc
     1621 tacggagtgt ccggggcggc aattttccca ttgcgaaggc ggggcgccgg gaagcaacag
     1681 ccatatcgcg tgccgggcta tccgtggacg ccgatggtat ttttgatagg ggccgcggcg
     1741 gttgtgggca acgcaatcta tctggcgacg aaagacccgc aacagtttcg ccacgtgctg
     1801 gccgcactgt ttctgatggc gttgggggtg ccggcctatt ttttttggaa cctacggaac
     1861 gcggacacag ccagcgatac cacactaccg agatgactcc ttttccgact ttgcgcactt
     1921 cgacagcatt ttgtttccat atcggctccg tagaaatcga ttcaccggct ccgagtaggt
     1981 agtggcaatt aaaaggccca gaaaacccgc cattaggatg acgacagtga acaaaatagg
     2041 cgcgaggcgc agtggcttgc cgagatacac aaataagtcg aagaacgcga agacgacgaa
     2101 catgtgagtc agatagacct catagctgtg cttcccgagt cccaggagcg gagccagaat
     2161 gcgcggactt ttccattgcg cttgggcggt ggccgcgatg aacatgcaag ttcccagagc
     2221 gaggatggtg atattgagtc aggttcgagc gggccagccc aggtaagctt gccaggaaaa
     2281 tatcaggctg aggctcaccg ttgccgtacc gcatgcgcca acaacccaaa gcgaccggac
     2341 cgaaaagcga acttgtgagg cgagcaacgc ggtgagacag cccagcgcga tggcttccat
     2401 gccccccaag taggagtatt cttcccaaac ttcgttgccg tgggcgaata cggtacggct
     2461 gaagggacca agcacaacga agatgagaag aatcgggaca aggaacctgc gactaccgga
     2521 cagccagcag accagaggga aaaagagata gaacatctcc tcgaccgaca aagaccaaag
     2581 gatgtcccaa ttcgcaggga gataaccttg cctggcctcg agcacattga catgaaaagt
     2641 gagtgctgca agaagagcgc ggccgagccc tcccgttttt gcgggcacaa cgaagtcctc
     2701 gattttcgca aaatgtagtg cggttaggac agcgagcact agcaggagca acggcccgat
     2761 ccgagcaaag cgcaacaaat agaagtcgcg cacgctgatt cgcgttagcg cgtcagatcg
     2821 tcgaagagac atggacgtga tcagaaatcc cgaaacgacg aagaacattt gaacgcctag
     2881 ctggccgttc cagaccagga aatgcacgag ttgttcgggc aagaatttca ggtatgggac
     2941 ctttgcgccg agcagacgga tgttgatgtg gttcatcagg acgaagaaaa tcgccaggcc
     3001 acggagcagg tcgatcccgt ccaggcgttt ccaagctgcg gtctgatttg tagaatccgc
     3061 cgacgccatg gatcattttc attcagagtt ggagatcaat cgcgccaatc atgggatgag
     3121 gagcgaaccc ataaccctta acagcgagag ggacgccaag gacgtccgtc tcagaatggc
     3181 ggctgctaca agagctggag ggggtccatg tcgaccagga gtgcggtttg cgggatctcc
     3241 ttcgagtcgg cgtaggagag ggcggcggcg aggagtttgg cgagggcggc ttttttccgg
     3301 gatttgagaa ggaactggaa acggtattcg gatttgagcc gggagagcgg ggcgctggcg
     3361 ggtcccagaa cgcggatttc gttgccggcg tgcggcgcga gaaattgcga gagctgtttg
     3421 gaccattgga tggctttg
//
LOCUS       DASTPM010000454         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_996366, whole genome shotgun sequence.
ACCESSION   DASTPM010000454 DASTPM010000000
VERSION     DASTPM010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996366"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFR42_08060"
     CDS             <1..353
                     /locus_tag="VFR42_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369146.1"
                     /translation="CPPRWLRNLYRFAPVPDITFYFRAPLDVAVHRILSGRPKLKYYE
                     AGMDLGLSYDRSESFRLFQARILEEYDKMVDTDNFVVLDGTIPVNKLQKQMRDIVASK
                     IDLKRFAPHAAETE"
     gene            356..1207
                     /gene="tmk"
                     /locus_tag="VFR42_08065"
     CDS             356..1207
                     /gene="tmk"
                     /locus_tag="VFR42_08065"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU0369147.1"
                     /translation="MPDQEKEKEKSGSTATTTADPATAPAPAPAATKTPTDEEAAQYF
                     YGEALVGFDPSEITGSLIVIEGMDGSGRSTQISLLQEWLESEGFAVQTSGLRRSNLVG
                     RDIDELLAKNAVTRLTLSLMYATDFFDQVEHRILPALRSGTVVLADRFIFTLIARGVV
                     RGINRDYISGLYAMALRPHLTFWLNVRPETAFAREFKKAQAISYWEAGRDMSLSHDLY
                     WSFIRYQTMIKREFEVMAKRHNFLELDGEASVSTVNKQLRQRIAEQLGIRATKYTPSA
                     ALAHLWR"
     gene            1217..>2334
                     /locus_tag="VFR42_08070"
     CDS             1217..>2334
                     /locus_tag="VFR42_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369148.1"
                     /translation="MSEPVHIAAIDAGSNAVRLSVARAYSALDIEPLHNERYSLRLGE
                     GVFLRHRFSEDIFKKGVKAFRHFKEVMDEFGVTRYRAVATSASREARNRDAFVRRIKS
                     KAGIALQVISGSEESRLGREAAIAALGPESPPRCIADLGGGSLEINILRDHTVEQNAQ
                     LPVGTVRLMTTLNIPGVIRPAQEEQVKRYVRALLESRLPSRPNLGESIAVALGGNAET
                     LMTVAPGPREHGVPTLQLSLLRERLDDILRRDVRERMKAYAVRRDRADVMGIAAITFV
                     TLGRYLNLRTLSIPGVGLREGLLQEIARDAFSRKEPHRYNAAARQLLVGTRSFARRME
                     YDQRHAEHVRELGILLFDVLQPVHHLPAESRVLLEAGA"
BASE COUNT          453 a          824 c          608 g          449 t
ORIGIN      
        1 gttgcccgcc gcgttggctg cgcaacctct atcgctttgc gccggtcccg gacatcactt
       61 tttattttcg cgcaccgctc gacgtggccg tgcaccgcat cctcagcggc cgccccaagc
      121 tcaaatatta cgaagctggc atggatctcg gcctctctta cgaccgctcc gagagttttc
      181 gcctatttca agcccgcatc ctcgaggaat acgacaagat ggtggacacc gataatttcg
      241 tggtcctcga cggcactatc ccggtcaaca aattgcaaaa gcagatgcgc gatatcgtgg
      301 cctccaaaat tgatttgaag cgctttgcgc cgcacgccgc ggaaacggaa tagccatgcc
      361 tgaccaggaa aaagaaaaag agaaatccgg atcaaccgcg accaccactg ctgatcctgc
      421 caccgcgcct gcgcccgccc cagccgcgac aaagacgccc accgacgaag aagccgccca
      481 atatttttac ggtgaagcgc tggtcggctt cgatcccagc gaaatcaccg gcagcctcat
      541 cgtcatcgaa ggcatggatg gctccggccg ctccacgcaa atttccttgc ttcaagaatg
      601 gcttgaatcc gaaggctttg ccgtgcagac cagcggcctg cgccgctcta acctggtagg
      661 ccgcgatatc gacgagctgc tcgcgaaaaa cgcagtcacc cggctgacgc tttccctgat
      721 gtacgccacc gacttcttcg atcaagtgga gcatcgcatc ctgcctgcgc tgcgttccgg
      781 cacggtggtt ctcgccgacc gcttcatttt cactttgatt gcccgcggcg tggtgcgcgg
      841 catcaatcgt gattacatca gtggcttgta cgccatggcc ttacgtccgc accttacgtt
      901 ttggctcaac gtgcgcccgg aaaccgcctt cgcccgcgaa ttcaagaaag cgcaagccat
      961 cagctactgg gaagccggcc gcgacatgtc tctctcccac gatctctatt ggtccttcat
     1021 ccgctaccag accatgatca agcgcgaatt cgaggtcatg gccaagcgcc acaattttct
     1081 cgagctggac ggcgaggcca gcgtctccac ggtcaacaag cagctccgcc agcgcatcgc
     1141 cgaacagctg ggcattcgcg ccacaaaata cactccttcc gctgcgttgg cccacctctg
     1201 gcggtaaact ctccgcatgt ctgagccggt ccacattgcc gccatcgacg ccggctccaa
     1261 cgccgtgcgc ctctccgtcg ctcgcgccta ctccgcgctg gacatcgagc cgttgcacaa
     1321 cgagcgctat tcgttgcgcc tcggcgaagg cgttttcctc cgccaccgct tcagtgaaga
     1381 cattttcaaa aaaggtgtga aggccttccg tcatttcaag gaagtgatgg acgaattcgg
     1441 cgtcacccgc tatcgcgccg tggccaccag cgcctcccgt gaagcgcgca accgcgatgc
     1501 tttcgttcgc cgcatcaaat cgaaagcagg gatcgctctc caggtcatca gcggcagcga
     1561 ggaatcccgc ctcggccgcg aagccgccat cgccgcactc ggccccgaat cgcctccgcg
     1621 ctgcattgcc gatctcggcg gcggcagcct tgaaatcaat attcttcgcg atcacaccgt
     1681 cgagcaaaac gcgcagctcc cagtcggcac cgtgcgcctg atgaccacgc tgaatattcc
     1741 cggcgtcatc cgccccgcgc aggaagagca agtgaagcgt tacgtccgcg cgctgctgga
     1801 atcgcgcctt ccctcgcggc ccaatctggg agaaagcatc gccgtggcac ttggaggcaa
     1861 cgccgaaact ttgatgaccg tcgcccctgg tccccgcgag cacggcgtcc ccactttgca
     1921 attatcgctg ctgcgcgagc gcctcgacga catcctccgc cgcgacgttc gcgagcgcat
     1981 gaaagcctac gccgtccgcc gcgatcgcgc cgacgtcatg ggcatcgccg ccatcacctt
     2041 cgtcaccctg ggccgctacc tcaatctccg cacgctttcc attcccggcg tcggcctgcg
     2101 cgaaggcctg ctccaggaaa tcgcacgcga cgctttctcc cgcaaggagc cgcaccgtta
     2161 caacgccgcc gcccgccagc ttctcgtcgg cacccgcagc ttcgcccgcc gcatggaata
     2221 cgatcagcgt cacgcggaac acgtccgaga actaggcatt ctgctcttcg atgtattgca
     2281 gcccgtgcat catcttcccg cggaatcgcg cgtcctgctg gaagccggcg cgtt
//
LOCUS       DASTPM010000455         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_477353, whole genome shotgun sequence.
ACCESSION   DASTPM010000455 DASTPM010000000
VERSION     DASTPM010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477353"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VFR42_08075"
     CDS             <1..1722
                     /locus_tag="VFR42_08075"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369149.1"
                     /translation="LVANRAAEQSDQTDEFPSASSSLWSDLDVLCLTAMHKDLRRRYA
                     SAEALLRDVDHYLKGEALEAQPDSARYRMRKFVTRNRRAVTAAAAAVVLTAGLITFFT
                     VRLAIARNAALAQAARTQRIQKFMTNLFQGGDEVAGPADSLRVVTLLDRGVQEAQSLN
                     AEPVVQAELYETLGGIYQKLGKLDQANTLLQSALDSHKSHSGRDSPEVAQTLVAIGQL
                     RSDQAQLDDAEKFTEQGLQMARRHLPADHPAVVKAMISYGRVLAQRGSYDHAIQVLNN
                     AVNIESQPGRPAADLVNSLSALAEANYSAGHYPVADSLYRRVLEMHRKMYGERHPAVA
                     DDLGNIAAIQQDLGFYTEAEKFDRQALDITQGYYGKEHPKTAINLTMLGRALEFENKF
                     DDSDAVLKQALAIQERVYGPVHPSVAETLNELAANESMRNDMDGAIVQFRRVAEIYRS
                     IYGDHHYLVAIALANVASQYMDKKDYASAEPIFRDVIRRLTEVLPPDNVNTGIARIKL
                     GRTLLRQNRFAEAEAQTHAGFDSLSKQMDPTVSWLRSARKDLVAEYTGMNQPDQAAKF
                     KEATKKQ"
     gene            complement(1750..>4857)
                     /locus_tag="VFR42_08080"
     CDS             complement(1750..>4857)
                     /locus_tag="VFR42_08080"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU0369150.1"
                     /translation="WERKRGKLLDLNNLLLGKSNNFSVIAGDQTLLDGFKYVITLDAD
                     TQLPRDSAYRLVGAMAHPMQRAVIHPVSNVVVEGYGILQPRVGISIHSANRSRLASLF
                     SGDSTFDVYTRAVSDVYQDLFGEGSFTGKGIYEIEVFQRVLEQRFPCNAILSHDMIEG
                     AYARAGLLSDVEVIDDYPSRIAAYSKRKHRWVRGDWQILFWLFPRVPDYFGNVVRNPI
                     NLISRWKILDNLRRSLTEIATLAILVSGWLFLPGNALYWTLATLGMFAAPTYLQFALA
                     ILRSGNSKFSARFWKEWFTDVFAANAHLFMRLVCLPQQSMVTFDAILRVVIRMAVTRR
                     KLLEWETAAEAESSGKKRSPVDIYLAATPWLAFLGGVFLAVDRPQVFLVALPLLLLWG
                     GSREFSNWLDQPLPEDGLKISDRDEALLRNSALRTWRFFREFSNERENYLIPDIFKEE
                     GKLTAHRVSTTNLGLLLNARVAAVDLGHLTVAEFIADTQKTLATVRRLPKAHGHLYNW
                     YDNRTLEPVGDPFLSTVDNGNLVCCLWTLKQACLEELKRPIFRKELWAGIRDHARLVQ
                     QLTPTKEVRKAVRGLEDSIAKRGETVLEWLSSLPALEQAAVTLAEAAKKNAQDSEELQ
                     WWLSEMSTRVSSLQTLAVQFAPWASTKFRTLRVEEKQQEKWIAELSLAEAVRVYDEIA
                     AGLEEENRGQQVESAAENVSRELLNDALQAAREAATKAAAALSEIAAYADELAAGMDF
                     SLVYDAARKLITIGFDAAKDAASEHHYDLLASEARAAVFAGVAKGEIPQECWFHLDRS
                     HVRFKGERVLQSWTGTMFEYLMPSLWMKSSAGTLLDRNMSSAVRAQQKHARRYGIPWG
                     ISESACSLISGDGHYHYFAFGVPALALHRPDDERVVISPYSTFMSLMTGSYDALRNLQ
                     RLKKMGAVGAYGFYEALDFGPRMTLHRKRFTMVRSWMAHHQGMCLLSIANVLCDSAVQ
                     RRFHAEPQVAATERILHERANASDTLDVSKAQRKRKAEALLTEGQSVLNPSYWEAKDR
                     AQSAA"
BASE COUNT         1030 a         1485 c         1370 g          972 t
ORIGIN      
        1 ctagtggcga atcgcgcggc cgaacagtcc gaccagaccg acgaatttcc ttcggcaagc
       61 agctcattgt ggtctgacct ggacgtcctc tgcctgaccg ccatgcacaa agatctgcgg
      121 cgcaggtatg cttcggcgga agcactgttg cgcgacgtcg atcattattt gaagggcgaa
      181 gctctggaag cgcagccgga cagcgctcgc tatcgcatgc gcaaatttgt tacgcgcaac
      241 aggcgagcgg tgacggccgc ggccgcagcc gtggtgctga ctgcagggct cattacattt
      301 tttacggtgc gtctggctat cgcgcgaaac gccgcgctgg cccaggctgc ccgcacacaa
      361 cgcatccaga aattcatgac caatttgttt caaggcggcg atgaagtcgc cggacccgcc
      421 gatagcctgc gcgtggtcac actcctcgac cgcggggtgc aggaagcgca gtcgttgaac
      481 gccgagccgg tggtacaggc cgaactttat gaaacgctgg gcggtatcta tcaaaaactt
      541 ggcaagctcg atcaggccaa cacgcttctg caatccgcgc tcgactcgca taaatctcac
      601 tccggccgag acagccccga ggtcgcgcaa actctggttg cgattggcca gttgcgctcc
      661 gatcaagccc agcttgacga tgccgagaaa ttcaccgagc aaggtttgca aatggcgcgc
      721 cgccatttac ccgccgacca ccccgctgtc gtaaaagcca tgatcagcta tggcagagtg
      781 ctggcgcagc gcggttcgta tgaccatgcc attcaagtgc tcaacaacgc cgtcaatatc
      841 gaatcccagc ccggcaggcc ggctgcggac ctggtgaaca gcctctccgc gctcgccgaa
      901 gcgaattatt ccgcggggca ttatccggtt gcggattctt tgtatcgtcg cgtgctggaa
      961 atgcaccgca aaatgtatgg agaacggcat cctgcggtcg ccgacgactt gggcaacatc
     1021 gcagccattc agcaggattt gggtttttat accgaggcag agaaatttga ccggcaagcg
     1081 ctcgatatca cgcaaggcta ctacgggaaa gaacatccca agaccgcgat caacttgacc
     1141 atgcttggac gcgccttgga gttcgaaaac aaattcgacg actccgacgc agtgctaaag
     1201 caagctctgg caatccagga acgcgtttac ggccccgtcc atccgtcagt ggcggaaacg
     1261 ctcaacgaac tggccgccaa cgagtccatg cgcaacgata tggacggcgc tatcgtgcag
     1321 ttccggcgtg tcgccgaaat ctatcgttcc atttatggcg accatcacta tctggttgcc
     1381 atcgctctgg ccaacgtcgc gagccaatac atggacaaaa aagactacgc cagtgccgag
     1441 ccgatttttc gcgacgtgat tcgtcgcttg acggaagttc ttcctcccga caacgtcaac
     1501 acaggcattg cgcgcatcaa gctgggccgc acgctattgc ggcaaaatcg ctttgccgaa
     1561 gcggaagcgc aaacccatgc aggcttcgac agtttgagca aacagatgga tccgacggtg
     1621 agctggttgc gcagcgcgcg caaggatctg gtcgcggaat ataccggcat gaatcagccc
     1681 gaccaagcgg cgaagtttaa ggaagccacc aaaaagcagt agcttcctgt gtttggtcat
     1741 gattgcgttt tacgcggcgc tttgcgcgcg atctttggct tcccaatagc tgggattcag
     1801 cacgctctgc ccctcggtga gcagcgcctc cgccttgcgt ttgcgttgcg ccttgctgac
     1861 gtcgagggta tccgaagcgt tggcgcgctc atgcaggatg cgctcggtgg cagcaacctg
     1921 cggttccgcg tggaatcgcc gttgcacagc cgaatcgcac agaacgttgg cgatcgagag
     1981 caggcacatg ccctggtgat gcgccatcca cgaacgcacc atggtgaaac gcttgcgatg
     2041 cagcgtcatg cgcgggccga agtccagcgc ttcgtaaaac ccataagcgc cgaccgcacc
     2101 cattttttta aggcgctgca agttgcgcag ggcgtcgtaa gagccggtca tcaagctcat
     2161 gaatgtcgaa tagggagaaa tcaccacgcg ttcgtcatcc ggccggtgca aagccagcgc
     2221 cggcacgcca aacgcgaagt agtgataatg tccgtcgccg ctgatcaagc tgcaggccga
     2281 ctcggaaatg ccccacggaa ttccgtaacg ccgcgcatgt ttctgctgtg cgcgcactgc
     2341 gctgctcatg ttgcgatcga gcagcgtgcc tgccgaagac ttcatccaca gcgacggcat
     2401 caggtattcg aacatggtgc cggtccacga ttgcagtacg cgttcgccct tgaaccgcac
     2461 atgtgagcga tcgaggtgga accagcattc ctgcggaatt tcgcccttgg ccacgccggc
     2521 aaaaactgcc gcgcgggctt cggaagccag caggtcgtaa tgatgttcgc tcgcggcgtc
     2581 tttggcagcg tcgaaaccga tggtgatcag ctttcgcgcc gcgtcgtaaa ccagcgaaaa
     2641 gtccatgccg gcggccaact catcggcata agcggcgatt tcgctcaatg ctgccgcggc
     2701 cttggttgcc gcttcccggg cggcctgcag ggcgtcattc agcaattcac gggaaacatt
     2761 ttcagcggca ctttccacct gctgcccgcg attctcttct tccaggccag ccgcgatttc
     2821 atcgtaaacg cgaaccgctt cagccaggct cagctcggca atccattttt cttgctgctt
     2881 ttcttcgacg cgcagcgtgc ggaacttcgt cgaagcccac ggcgcaaact gcaccgccaa
     2941 ggtttgcagc gaggaaactc gcgtgctcat ttcgctgagc caccactgca gctcttcgga
     3001 gtcttgcgca ttcttcttgg ccgcctcggc cagagtaact gcagcctgct ccagagcggg
     3061 cagggaactc agccactcca acaccgtttc gccgcgcttg gcgatcgaat cttccagccc
     3121 gcgcaccgct tttcgaactt ctttcgtcgg cgtaagttgt tgcaccaacc gcgcgtggtc
     3181 gcgtatgcca gcccatagtt ctttgcggaa aatcgggcgt ttcagttctt ccaggcaggc
     3241 ttgcttcaag gtccacaggc agcaaaccaa gttgccgttg tcgacggtgg acagaaatgg
     3301 atcccccacc ggttccagcg tgcggttgtc ataccagttg taaagatgtc cgtgcgcttt
     3361 gggcaggcgc cgcaccgtcg ccagcgtctt ctgtgtgtcg gcaatgaatt ccgcaacggt
     3421 caggtggccc aaatccacgg ctgccacgcg cgcgttcagc aacaatccga ggttggtagt
     3481 agacacccgg tgcgccgtca gctttccttc ctccttgaag atgtccggga tcaaataatt
     3541 ttcccgttcg ttgctgaatt cgcggaagaa cctccacgtg cgcagcgcgg agttgcgcaa
     3601 cagcgcttcg tcacggtcgc tgatcttcag gccgtcttcc ggtagcggct ggtccagcca
     3661 gttggaaaat tcccgcgagc ctccccacag caacaacagt ggcaaagcca ccaggaacac
     3721 ctgcggccgg tccaccgcca ggaacacgcc gcccaaaaaa gccagccacg gcgtggccgc
     3781 caggtagata tccaccggac tgcgcttttt gccgctggat tccgcttccg ccgcagtctc
     3841 ccattccagc aacttccggc gggtcaccgc catgcgaatc accacccgca aaatcgcgtc
     3901 aaacgtcacc atgctctgtt gtggcaagca gaccaatcgc atgaacaggt gcgcgttcgc
     3961 agcgaaaacg tccgtgaacc actctttcca gaatcgcgcg ctaaatttgc tgttgccgct
     4021 gcgcaaaatc gccagcgcga attgcaggta ggtaggcgcc gcaaacatgc cgagcgtagc
     4081 caacgtccaa tagagcgcat tccccggcag aaacagccag ccgcttacca gaatggccag
     4141 tgtggcgatt tccgtcaggc tgcgccgcaa gttgtccaga atcttccagc gcgaaatcag
     4201 gttgatcgga ttgcgcacca cgttgccgaa ataatccgga acacgcggga acagccagaa
     4261 aagaatttgc cagtcaccgc gtacccagcg gtgtttgcgc ttgctgtatg cggcgatgcg
     4321 cgagggatag tcgtcaatca cttccacgtc ggaaaggagg ccagcgcgcg cgtacgcgcc
     4381 ttcaatcatg tcgtggctca ggattgcgtt gcacggaaag cgctgctcca gcacgcgctg
     4441 gaacacttcg atttcgtaaa tgccttttcc ggtgaagctg ccttcgccga ataggtcctg
     4501 gtagacgtcg gaaaccgcac gcgtgtagac atcgaatgtg gaatcgcccg agaaaaggga
     4561 agccaggcgt gaacggttcg ccgaatggat gctgatgccc actcgcggct gcaggattcc
     4621 gtagccctcg accaccacgt tggaaaccgg gtgaatcacc gcgcgctgca tcggatgggc
     4681 catcgcaccc accagccggt aggcggagtc tctcggcagt tgggtgtcgg cgtccaaagt
     4741 gatgacgtat ttaaagccgt ctagcagcgt ctgatcgccc gcgatcaccg agaagttatt
     4801 agattttccg agcagcaggt tgttcagatc gaggagcttt ccgcgcttgc gctccca
//
LOCUS       DASTPM010000456         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_881079, whole genome shotgun sequence.
ACCESSION   DASTPM010000456 DASTPM010000000
VERSION     DASTPM010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881079"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFR42_08085"
     CDS             <1..745
                     /locus_tag="VFR42_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369151.1"
                     /translation="SGILLGLATACKWNGLFSIGALVVIGCIVYMRRRNFNLRLLLVS
                     FSVLPAAAYSATFICLFRYSGTPFTLSNLFAIQGRMYRFMAALPGNGFIHVPWFVWPF
                     EIAPQRGLMYLVGNYAVMFSGLLALVVCGWRVARRFAVPELTAASATNVAPELTPELA
                     IVGLYLVNLMQWVVIPHKIVCYYYYYPCALLLSLALVLAFHRSERKQLFGIRLSIVPV
                     VAAIAIFLFSYSHMASLEAPWDTVLGYWR"
     gene            902..1090
                     /locus_tag="VFR42_08090"
     CDS             902..1090
                     /locus_tag="VFR42_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369152.1"
                     /translation="MCVFVQNIPSSSLFPFLICETALGKTGRPSGRAEVFQQMVTVAY
                     SGVEFDEQGLVLAIQRFL"
     gene            complement(1662..1838)
                     /locus_tag="VFR42_08095"
     CDS             complement(1662..1838)
                     /locus_tag="VFR42_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369153.1"
                     /translation="MGKLAAMMSEDEEIRFPDAVQIEPNLAARQELDSFNSVILEPMK
                     ESGTPADLFGGAAK"
     gene            complement(2047..>2238)
                     /locus_tag="VFR42_08100"
     CDS             complement(2047..>2238)
                     /locus_tag="VFR42_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369154.1"
                     /translation="DSRPVQPLTRYKLGTATMVDAHELDQVPQIPGFYSPLRMEFSGQ
                     RKIWSRLPSQERNASTSGE"
BASE COUNT          419 a          681 c          609 g          529 t
ORIGIN      
        1 gagcggcatt cttctgggcc ttgccacggc gtgcaagtgg aacgggcttt tttcgatcgg
       61 cgcgctcgtg gtgatcggct gcatcgtgta catgcggcgg cgcaacttca atttgcggtt
      121 actgctcgtg agcttttcgg tacttcccgc ggcggcgtac agtgcgacgt tcatctgctt
      181 attccgttac tcgggcacgc ccttcaccct atcgaatttg ttcgcaatcc agggccggat
      241 gtaccgcttc atggccgcgc ttcccggaaa tggtttcatc cacgttccct ggttcgtgtg
      301 gcctttcgag atcgcaccgc agcgcggact gatgtacctg gtcggcaact acgcggtcat
      361 gttcagcgga ttactggcgc tcgttgtctg cgggtggcgc gtggcccggc ggttcgcggt
      421 acccgaattg acggccgcat cggcaaccaa tgtagcgccc gaactgacgc ccgagttggc
      481 catcgtcggc ctctacttgg tcaacctgat gcagtgggtg gtgatcccgc acaaaatcgt
      541 ctgctactat tattattacc cctgcgctct tcttctgagc ctcgctctgg tcttggcgtt
      601 ccatcgctcg gagcgtaaac agctttttgg cattcgcctt agtatcgttc ctgtcgtcgc
      661 cgcgatcgca attttcctgt tcagttattc gcacatggcg agcctggaag ctccctggga
      721 caccgtcctg ggctactggc gttagacgac caggcggttc aagacaaagt gagaagccga
      781 gaaggaacga catcggctcc atcagaattt gaagtcttta gccccggaga gtaagcggta
      841 aagtccactc cgcccactat ctggacccgg taggccaatc cactcgataa agaaaagcta
      901 aatgtgtgtc ttcgttcaaa acattccgag ctcctctctt ttcccgttcc tcatctgcga
      961 gaccgcgttg ggcaagaccg gccgcccgtc tggccgcgca gaagttttcc agcaaatggt
     1021 cacggtggcg tacagcggcg tcgaattcga cgagcaaggc ctggtgctcg cgattcagcg
     1081 attcctttga ttgcgtcctc cgccgcgatg cacaaagact cgttcaccca gcacatgtgc
     1141 gctgagcgaa aatcccacaa tgattttttt gcttgttcca cgcgctgttc ggccgccgcc
     1201 agttccaatt gcgccgtttc aaaaagcgta ggcgtcaccg gttgttcgag ctgaagtagt
     1261 gtcaacgtca tgcgcggagt ctggcaaacg atcctctggt gacaaagaga aattcaggaa
     1321 tcggtgcggc ccgaggcact cccccagcca gcgcgcgggc gagttctatt cgccgccgag
     1381 cgcctcttca atttctcgcc ccaacgctgc aagcgcttcg ttcgctggtt cgcttcggtg
     1441 tcgcacagga aactggtaca tttgccagtt gcgatggtcg gtacctcacc tgtagcctcg
     1501 aaaaatgtct gacaaagagc gaggctacgc tgaggtgatc cggactcagg gggccgagtt
     1561 tctcggaatt cagcccggtc ttcgcggacc attggtactg tttaccgacc ctgtatcgcg
     1621 gaccacttta gcggttcacg aattggagtt cagcccccaa gctatttcgc agcgcctcca
     1681 aacaagtcgg caggcgttcc actttctttc ataggttcaa gaatcacact gttaaaactg
     1741 tcaagctcct gacgtgccgc cagattcggc tcgatttgaa ccgcgtccgg aaaccggatt
     1801 tcttcgtctt cgctcatcat cgccgccaac ttgcccaccg cgtgagaaat ccgcgatcga
     1861 taccgccggc cgattgctcg cgcgacgcca cctgctcgcc gtgggcgcat gagatttcgc
     1921 gaaactcaac gccgccgctc ttcccacggc tcgctaccac gaacgaatcc acacggtgca
     1981 ctgtcgcacg ctgcagctgg cttttttgca ccccgcagga atcacacttc tcgtcggcgc
     2041 acggaatcac tcgccagacg tgctcgcatt ccgctcctgg ctcggcaatc ggctccaaat
     2101 tttacgctgg ccggaaaatt ccatccggag cgggctgtag aacccaggga tttgcggcac
     2161 ttggtcaagc tcatgtgcat cgaccatggt tgccgtgccg agtttgtagc gcgtgagtgg
     2221 ttgaaccggc cgggagtc
//
LOCUS       DASTPM010000457         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_765125, whole genome shotgun sequence.
ACCESSION   DASTPM010000457 DASTPM010000000
VERSION     DASTPM010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765125"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /gene="yidC"
                     /locus_tag="VFR42_08105"
     CDS             <1..1137
                     /gene="yidC"
                     /locus_tag="VFR42_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU0369155.1"
                     /translation="FDGKPITAGLSWLGGFGDVTVTNPAPVETVNAYYSEAGKLNSFP
                     YKKLDDGGKWGPGVWQGGKDWAGIEDRYFAAAFLAPVGAPPGTLETRYWKAWHTAKVT
                     DNQGKPEDKPEPVPQVAVASSAQPAAMRLYVGPKDYDALKKMNPPLQALVNFGWLEII
                     ADPLFHALKWIHSYVPNWGWAIVILTLVVNMLLFPLRISGYKTTMKMQRVAPEIKAIQ
                     ERYKKYKINDPRKAEMNQEVMAIYQREGVNPVSGCLQAFAQMPIWFGLNRALSSAIEM
                     RHAPWFGWITDLSASDHYRVLPILMGISMYIVSKMTPMTTTDPQQQMMMKFMPISFAA
                     MFMFFPISSGLAVYILTSSVVGIGQQWYLNRTHPVTPAPKAGKK"
     gene            1394..1924
                     /locus_tag="VFR42_08110"
     CDS             1394..1924
                     /locus_tag="VFR42_08110"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEU0369156.1"
                     /translation="MTYNLIENGQLNRSAAAEALRQFLEETIRAGKFDLKASVRALDP
                     SVAPDEGGAEIFADLTGRDNELLLERGAELLQSIEHLAHRALRLEPPFQDKIFLDCAG
                     YRALRVEELKMMARAAAERVQTSRQPFKLNPMNSRERRIVHLALQSVPGIRTESVGVG
                     PTRQVVIHPANPTPGR"
     gene            2028..>3442
                     /locus_tag="VFR42_08115"
     CDS             2028..>3442
                     /locus_tag="VFR42_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0369157.1"
                     /translation="MRKPLLILSVLCLSVSGGLVAAQQPPDKPLFRLQEVMIPMRDGV
                     RLQTAILTPADQQGPLPILFRRTPYGVPERPPEQMPASWKELAQDGYIFVIQNLRGRF
                     KSEGNFDLTSQVDLSNPKAKNETTDAYDSIEWLVKNVPNNNGKVGMYGVSYDGLTTAL
                     ALLHPHPALKAMSEQASPVDQWMNDDNHRYGALRESYDFEYAVMEQADKNSNTHFAFE
                     TYDTYEWYLNLGPVSNINAKYLHGKIPYWNSTVEHPDYDEFWKKEAWVSQLHASTVPN
                     LNVAGFSDQEDPWGPWQIFRHAAEHDPDHTNFIVAGPWFHGQWQTPKGDSIGLIPFAG
                     HETAREFRETIEAPFFRYYLHGKGEKPAWRATTFQSGSNTWRTYAAWPPADVRPTNLY
                     LHADGTLSITAPSMNEKSYREYVSDPANPVPYRQRPISPTYPAGDWRTWEVADQRFVE
                     HRPDVLSFASAPLEHDLAITGP"
BASE COUNT          746 a         1152 c          831 g          713 t
ORIGIN      
        1 ttcgacggca agcccatcac cgccggcctc tcctggctcg gtggcttcgg cgatgtcacg
       61 gtcaccaatc ccgctcccgt cgaaaccgtc aacgcgtatt acagcgaggc tggcaagctc
      121 aacagctttc cctacaagaa actcgacgat ggcggcaaat ggggccccgg cgtttggcaa
      181 ggcggtaaag attgggccgg cattgaagat cgctatttcg ctgccgcgtt tttagcgcct
      241 gtcggggcac caccgggcac gctcgagacc cgctactgga aagcttggca caccgctaag
      301 gtcaccgaca accagggcaa gcccgaagac aaacccgagc ctgtccctca ggtcgctgtt
      361 gcctcatccg cgcagcccgc cgctatgcgc ctctatgtcg gccccaagga ttacgacgcc
      421 ctgaaaaaaa tgaatccgcc gctacaggcg ctggttaatt tcggctggct cgaaatcatc
      481 gccgatccac tcttccacgc actcaagtgg attcacagct acgtcccgaa ctggggctgg
      541 gccatcgtca ttctcacgct cgtcgtcaac atgctgctct tcccgctgcg catctccggc
      601 tacaaaacca ccatgaagat gcagcgcgtc gctccggaaa tcaaagccat ccaggaacgc
      661 tataagaaat acaaaatcaa cgacccgcgc aaagccgaaa tgaatcagga agtcatggcc
      721 atctatcagc gcgaaggcgt caacccggtc agtggctgcc tgcaggcttt cgctcagatg
      781 cccatctggt ttggactcaa ccgcgcgctc agctccgcca tcgaaatgcg ccatgccccc
      841 tggtttggtt ggattaccga cctttccgct tctgaccact atcgcgtctt gcccatcctc
      901 atgggcatct cgatgtacat cgtctccaag atgacgccca tgaccactac cgatccacag
      961 cagcagatga tgatgaaatt catgcccatc tccttcgctg ccatgttcat gttcttcccc
     1021 atctccagcg gtctcgccgt ctacatttta accagcagcg tcgtcggtat cggtcagcag
     1081 tggtacctga atcgcactca tcccgtgacg ccggcgccaa aagcaggcaa aaaatagaca
     1141 cgcccttcct acttgtcatc ccgaccggcg cacgcaaacc gggttccagg gcttgtacct
     1201 gcaaaccttg aattaggtga gtcgcgtgcc caatggcttg ccccggctct tgtcgggcag
     1261 gaactaggcc agaacgcccg gctagcaatg tggagacgaa tcttccctgc tttcctccat
     1321 ttctccttcc gactttttca gccgctccct cacaacctgt cgtaggatat gaggacgccc
     1381 gtcgtcccca aaaatgacct ataacctgat cgaaaatggc caactgaatc gctctgcggc
     1441 tgcagaggcc ctgcgccaat tcctggaaga aaccattcgc gccggaaaat ttgatctgaa
     1501 agcaagtgtc cgcgcgctcg atcccagtgt ggctccggac gagggaggcg ccgaaatttt
     1561 cgccgatctc accggccgcg ataacgaatt actccttgaa cgtggcgccg aacttctcca
     1621 gtccatcgag catctcgccc atcgcgcttt gcgcctcgag ccgccctttc aggacaaaat
     1681 ctttctcgac tgcgccggct atcgcgccct tcgcgtcgaa gaactaaaaa tgatggctcg
     1741 tgctgctgcc gaacgagtgc aaacttcgcg ccagcctttc aagctcaatc cgatgaactc
     1801 tcgcgaacgc cgcatcgtcc acctcgccct tcaatccgtt cccggcatcc gcactgaaag
     1861 cgtcggcgtc ggccccactc gccaggtcgt catccatccg gctaatccaa ctccgggccg
     1921 ttaacccgcc aatcttgcgc ttgcgaccgc tccaccaaaa cggtagcgcc gcaaaattgt
     1981 tatactcccg cctcgtccca tcggtgttca gattcgggga ggccagcgtg cgcaaaccat
     2041 tgcttatttt gtcggtcctc tgcctttccg tctccggagg ccttgtcgct gcgcagcagc
     2101 cccccgacaa gcctcttttt cgccttcagg aagtcatgat tcccatgcgc gacggcgttc
     2161 gcctgcaaac ggcgatcctc acgccggccg atcagcaggg tccactgccg attcttttcc
     2221 gccgcacgcc gtatggcgtt ccggaaaggc cgcccgaaca aatgccggcg agctggaagg
     2281 aactggcaca agacggctac atcttcgtca ttcagaatct gcgtggtcgt ttcaaatccg
     2341 aaggcaattt cgacctcacc tcgcaagtcg acctctctaa tcccaaagcc aaaaacgaaa
     2401 ctaccgacgc ctacgacagt atcgaatggc tcgtcaaaaa cgtgcccaac aacaacggca
     2461 aagtcggtat gtatggcgtc tcctacgatg gcctcactac cgccctcgcg ttactgcatc
     2521 cgcatcccgc tctcaaagct atgagcgagc aggcttcgcc cgttgatcaa tggatgaatg
     2581 atgacaacca ccgctacggc gcgttgcgcg agagctacga tttcgaatat gccgtcatgg
     2641 agcaagccga taagaacagc aacacgcact tcgctttcga gacttacgac acctatgagt
     2701 ggtacctgaa tctcggcccg gtctccaata tcaacgccaa atatctccac gggaaaattc
     2761 cttattggaa ttccaccgtc gagcatcccg attacgacga attttggaag aaagaagcgt
     2821 gggtcagcca gctgcacgcc tccaccgtcc ccaatctcaa cgttgccggc ttttcggacc
     2881 aggaagatcc ctggggaccg tggcaaattt tccgtcatgc tgccgaacac gatcccgatc
     2941 acaccaattt catcgtcgcc ggcccgtggt tccacgggca gtggcagacg cccaaaggtg
     3001 acagcatcgg tctgattccc ttcgctggcc acgaaaccgc tcgcgaattt cgcgaaacga
     3061 tcgaagcgcc gttcttccgc tattacctcc acggcaaagg cgaaaagccc gcctggcgcg
     3121 ccaccacgtt tcagagcggc tccaacacct ggcgcactta cgctgcatgg ccgcctgccg
     3181 acgtccggcc caccaacctt tatctgcacg cggacggaac tctttctatc accgcgccaa
     3241 gtatgaatga aaagtcttat cgggaatatg tttccgatcc agccaatcct gtcccgtatc
     3301 gccagcgtcc catttcgccc acttacccgg cgggggactg gcgaacctgg gaagttgcgg
     3361 atcagcgttt cgtggagcat cgccccgacg ttctttcttt tgccagcgct ccgctcgaac
     3421 acgatctcgc catcaccggc cc
//
LOCUS       DASTPM010000458         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_304288, whole genome shotgun sequence.
ACCESSION   DASTPM010000458 DASTPM010000000
VERSION     DASTPM010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304288"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1693
                     /locus_tag="VFR42_08120"
     CDS             <1..1693
                     /locus_tag="VFR42_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369158.1"
                     /translation="EDAIVVSEKLVKEDYYTSIHIEEFEVDGRDTKLGPEEITRDIPN
                     VSETMLRNLDEAGVIRIGAGIKQGDILVGKVTPKGETQLTPEEKLLRAIFGEKAGDVR
                     DASLYCPPGIEGVVVDVKIFSRKGQEKDERAKAIEEQQIAKLEKNLSDEIRILTDERL
                     KRLEGLLGGKEVQADLHDERTNKRLLTKGAILDRDTIERISTRNLKRIKYNDKDPRVN
                     EQIDEIEEMTSRQIDVLKKIVNEKKDKLTKGDELPPGVIKLVKVYVAMKRKLSVGDKM
                     AGRHGNKGVIARILPEEDMPYLDDGTPVEIVLNPLGVPSRMNVGQILETHLGWAGFEL
                     GKKITRILAENTRAEAIRRELKALFKDTALADTVAAFSDEEVEAVAPTLAFGVFSGSP
                     VFDGAREHEIKTLLERAGLPTSGKTFLHDGMTGDKFEQPVTVGYIYMLKLSHLVDDKI
                     HARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAAFILQELLTAKSDDVYGR
                     TKIYEAIVKGEAAMEPGVPESFNVLIRELQSLCLDVELIKAALGEKKPVAPVAAD"
     gene            1953..>2324
                     /locus_tag="VFR42_08125"
     CDS             1953..>2324
                     /locus_tag="VFR42_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369159.1"
                     /translation="MANPIADFDAIRISLASPEKIRSWSHGEVTKPETINYRTFKPER
                     DGLFCARIFGPVTDWECLCGKYKRMKHRGVICDKCGVEVTLSKVRRERLGHIELASPC
                     SHVWFFKGLPSRIGHLLDIALR"
BASE COUNT          521 a          657 c          748 g          398 t
ORIGIN      
        1 cgaggacgcc atcgtggtgt ccgagaagct ggtcaaggag gactactaca cctctattca
       61 catcgaggag tttgaagtcg acggccgcga caccaagctc ggtcccgagg agatcacgcg
      121 cgacatcccg aacgtcagcg agaccatgct gcgcaatctg gacgaagcag gtgtgattcg
      181 catcggcgcc ggcatcaagc agggcgacat cctggtgggc aaagtgacgc ccaagggcga
      241 aacccagctc acgccggaag agaaactgct gcgcgcgatc ttcggcgaaa aagccggcga
      301 cgtgcgcgac gcttcgctct attgcccgcc cggaatcgag ggcgtggtgg tggacgtgaa
      361 gatcttctcg cgcaaggggc aggaaaagga cgagcgggcg aaggcgatcg aagaacagca
      421 gatcgcaaag ctcgaaaaga acctctcgga cgagatccga attttgaccg acgagcgcct
      481 gaagcgtctg gaaggcttgc tgggtggtaa ggaagtgcag gctgatctgc atgacgagcg
      541 caccaacaag cggcttctga ccaagggcgc gattctcgat cgcgatacga tcgagcgcat
      601 ctcgacccgt aacctgaagc gcatcaagta caacgataag gatccgcggg tgaacgagca
      661 gatcgacgag atcgaggaaa tgacctcgcg ccagatcgat gtgctcaaga agatcgtcaa
      721 cgagaagaag gacaaactca ccaaggggga cgaactgccg ccgggggtga tcaagctggt
      781 caaagtctac gtcgccatga agcgcaagct gagcgtcggc gacaagatgg ccggccggca
      841 cgggaacaag ggcgtcatcg cccgcatcct gccggaagag gacatgccgt accttgacga
      901 cgggacaccg gtagaaatcg ttctgaaccc gctgggcgtg cccagccgta tgaacgtcgg
      961 ccagatcctg gagacgcact tgggttgggc gggttttgag ctgggcaaga agatcacccg
     1021 gatcctcgcg gagaacaccc gcgcggaagc catccgccgt gagctgaagg ctctgttcaa
     1081 ggacaccgcg ctggcggaca cggtggcggc cttcagcgac gaagaagtcg aagcggtggc
     1141 gccaacgctg gccttcggcg tcttctcggg atcgccggtg tttgacggcg cgcgcgaaca
     1201 cgaaatcaag acgctgctcg agcgggcagg cctgccgacc tccggcaaga ccttcctgca
     1261 cgacggcatg accggcgaca agttcgaaca gccggtcacg gtgggctaca tctacatgct
     1321 gaaactgtcg cacctggtgg acgacaagat tcacgctcgc tccatcggac cgtactcgct
     1381 gatcacgcag cagccgctgg gtggtaaggc gcagttcggc ggccagcgct tcggagaaat
     1441 ggaagtctgg gcgctggaag cgtacggcgc ggcgttcatc ctgcaggaac tgctcaccgc
     1501 gaagtcggac gacgtgtatg gccgcaccaa gatttacgag gccatcgtga agggcgaggc
     1561 cgccatggaa ccgggcgtcc ccgaatcctt caacgtgctg attcgcgaat tgcagtcact
     1621 ctgcctggat gtggaactga tcaaggcggc actgggcgag aagaagccgg tggcgccggt
     1681 ggcggcagac taggaaagca gttctcggtt ctcagttccc ggttctcagt tggtctgagg
     1741 cggggctgag agccggcgaa ctctttcaac agatttttga aacatgagcc actgcggcat
     1801 ctgctctcgg tgccgcaggt gggcgaaagt tctacccggt ttttgagaac tgagaaccgg
     1861 gaactgagaa ctggttttcg agggccgtca gcgggcgcgt gagaactcgc aggaagcgga
     1921 ggatcacctt gtatcgttcg agcccctttg acatggcgaa cccgattgcc gatttcgatg
     1981 ctatccgcat cagcctggca tctccggaaa aaatccggag ctggtcgcat ggtgaagtca
     2041 ctaagcccga aaccattaac taccgcacct ttaagcccga gcgcgacggc ctgttttgcg
     2101 cgcgtatttt tggaccggta accgactggg aatgcttgtg cggtaagtac aagcgcatga
     2161 agcaccgggg cgtgatctgc gacaagtgcg gcgtggaagt cacgctgtcg aaagttcgcc
     2221 gggaacgcct gggccatatc gaactggctt ccccctgctc gcacgtgtgg ttcttcaagg
     2281 gcctccccag ccgcatcggc cacctgcttg acatcgcact gcgc
//
LOCUS       DASTPM010000459         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1371393, whole genome shotgun sequence.
ACCESSION   DASTPM010000459 DASTPM010000000
VERSION     DASTPM010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371393"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFR42_08130"
     CDS             complement(<1..519)
                     /locus_tag="VFR42_08130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEU0369160.1"
                     /translation="MAKLTPPHGGAGLFGDPAPDAKPAPWRANIDGGSRGNPGPAAYG
                     VVIRDPRGEIVARLKKYIGRTTNNVAEYYGLIAALDYAQSNSIRALHVESDSELLVKQ
                     MRGQYKVKSADLQPLFERAKKMSQSFAFFQIRHVYREQNREADALANEAMNEVSGARP
                     APDNGKAKMENRN"
     gene            complement(533..1288)
                     /locus_tag="VFR42_08135"
     CDS             complement(533..1288)
                     /locus_tag="VFR42_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEU0369161.1"
                     /translation="MHPAIPQLLRLQTTDQEIAVFRAELDAAPKRIRELDQKLTGARS
                     AVTAAKEAHAQVVSARKKAEFEAAEWRERGKKFRSQSSAVKTNEAYKALQHEIANADA
                     EASKAEDAQLEQMMAAEEAEKKVKAAEAALQEAEQSMAAERKAIENHARESNRKMLAD
                     IPVRDQLAAEIPEEIVTLYTRAAKRHHGVALAEAVTEQCRGCGMRLLPRLYQQVYQPQ
                     DHDIHTCENCGRILYAAEPSPAGIPSPSAASAS"
     gene            1456..1785
                     /locus_tag="VFR42_08140"
     CDS             1456..1785
                     /locus_tag="VFR42_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0369162.1"
                     /translation="MGKPSDLVQGTLDLLILKTISLEPKHGWAIAKRIQQISGDVLQV
                     QQGSLYPALHRLEQRSWIKAKWRESETGRKAKFYSLTAAGRAQLDKEAENWSRLSAAI
                     NLVVETS"
     gene            1823..>2799
                     /locus_tag="VFR42_08145"
     CDS             1823..>2799
                     /locus_tag="VFR42_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369163.1"
                     /translation="MNWTRRAWTRLQALFQERENTRRLDDEIQFHLEQQIAENLAAGM
                     DKKEAHRAALRAFGNPTLVKEETKDAWGWIRVEQIARDLRNGARSLWRTPRFALVAIF
                     VMALGIGATTALFTVVRSVILEPLPFKEPSRLLHLYEYLEEGFPYNASAGGVFAEWKK
                     QSTQFSDLAIYSYRTEYNLSGTGGQLPEKVRGAECSWNLFATLGVEPALGRKFTAEDD
                     QPSATATVVLSWGLWKRRFGGETSILGQAIRLDAKAYTVIGIMPAWFAYPEQSAQLWT
                     PIYHEEPAVEMQALDSHDFASIGRLKPGASEAEGRAELAVIVKRLHEEHR"
BASE COUNT          565 a          727 c          891 g          616 t
ORIGIN      
        1 attgcgattt tccattttcg cttttccatt atccggcgca ggccgcgctc cggagacttc
       61 gttcatagcc tcgttggcga gtgcatccgc ttcgcgattc tgttcgcgat aaacatgccg
      121 tatctggaag aaggcgaacg actgcgacat tttttttgcg cgttcaaaga gcggctgcaa
      181 atcggcactc ttcaccttgt actggccgcg catctgcttc accagcaact cggagtcgct
      241 ctcgacgtgt agcgcgcgaa tgctgttgga ttgcgcgtag tcgagcgccg caattaatcc
      301 gtagtattcg gcgacgttgt tggtggtgcg gccgatgtac ttctttaggc gcgcgacaat
      361 ttcgccgcgc ggatcgcgga tcaccacgcc atacgctgcg ggccctggat tcccgcgcga
      421 ccctccgtca atattggcgc gccagggcgc aggttttgcg tccggagctg gatcgccgaa
      481 aagtcccgcg ccaccatggg gtggagtcag cttcgccata aaaacactcc catcatgaag
      541 ccgacgccgc gcttggactt ggaattcccg cgggactagg ttcggcggca tagagaatgc
      601 ggccgcagtt ttcgcaggtg tggatgtcgt gatcctgcgg ctggtaaacc tgctggtaga
      661 gacggggaag caggcgcata ccacagccgc gacactgttc ggttacggct tcggccagcg
      721 caacgccatg gtgccgcttg gcggcgcggg tgtacagggt gacgatttct tcgggaattt
      781 ccgccgccag ttggtcgcga acaggaatat cggccagcat tttgcggttg ctttcgcgag
      841 cgtgattttc gatagccttg cgttcggcgg ccatcgattg ctcggcttct tgcagagccg
      901 cttccgccgc tttcactttt ttttcggctt cttccgcggc catcatctgt tcgagctgcg
      961 cgtcttcggc tttactggct tccgcgtcgg cgttggctat ttcatgctgc agcgctttgt
     1021 aggcttcgtt ggtttttacc gcagagctct gcgaacggaa tttcttgcca cgctcgcgcc
     1081 actcggcagc ttcaaattcg gctttcttgc gcgcggacac cacttgcgcg tgcgcttcct
     1141 tggcggcagt gacggcgctg cgcgcgccgg tcagtttctg gtccagttcg cggatgcgtt
     1201 tgggagcggc gtccagttcg gcgcggaaca ctgcaatttc ctgatcggtc gtctggagtc
     1261 gcaagagttg cggaatcgcg ggatgcacgg ggaaagttta gcacagaggg ttggacagct
     1321 tagggaagct gaggggcgga gagattgctc ccgacgagag ctggaatcta ccatcgcttc
     1381 gcgcggaatg accgaagcgc gccaagcggg tccatatctt gactatctac aattgatgct
     1441 gtagatagtc atagtatggg aaaaccaagt gatctggtgc agggaacatt ggatcttctg
     1501 attctgaaaa cgatctcgct cgagccgaag catggctggg cgattgccaa gcgcattcag
     1561 cagatttcgg gggatgtgct gcaggtgcag cagggatcgc tataccccgc tctgcaccgg
     1621 ttggagcagc gcagctggat caaggcgaaa tggagagaat ccgagacggg cagaaaagcg
     1681 aagttttact cgctcacggc cgcgggccgg gcgcagcttg acaaagaagc ggagaactgg
     1741 agccgccttt cggccgcaat caacctggtg gtcgaaacat catgatgagt tcacgtatcg
     1801 aatagacgct ggagcggttg atatgaactg gacgaggcga gcatggacgc gattgcaggc
     1861 gctgtttcaa gagcgtgaga atacgcggcg gctggatgac gaaattcaat ttcatttgga
     1921 gcagcagatt gcggagaacc tggcagcggg gatggacaag aaagaagcgc accgcgcagc
     1981 gttgcgagcc tttggcaatc ctacgctggt gaaggaagag accaaggatg cctggggatg
     2041 gattcgcgta gagcagatcg cgcgcgattt gcggaacgga gcgcggtcgc tttggcgaac
     2101 gccaaggttt gccttggtgg cgatcttcgt gatggcgctg ggcattggag cgacgacggc
     2161 gctgttcaca gtggtgcgtt cggtgatcct ggaacccctg ccgttcaagg agccgtcgcg
     2221 actgttgcat ctctacgaat accttgagga aggatttcct tacaacgcat cagcgggcgg
     2281 cgtatttgcc gaatggaaga aacagagcac gcaattttcc gatttagcga tttattccta
     2341 ccggacggaa tacaaccttt cagggacggg cggacagctt cccgagaaag ttcgcggagc
     2401 agagtgttcg tggaacctgt tcgcgaccct tggagtggaa ccggcgttgg gtcggaagtt
     2461 tacagcggaa gacgaccaac cgtccgcgac tgcgacggtg gttctgagtt ggggactttg
     2521 gaaaaggcgc tttggaggag aaacctcaat cctgggccaa gcgatacgtc tggatgcgaa
     2581 ggcatacacg gtgatcggca tcatgcccgc atggtttgcg tatccggagc agagcgcaca
     2641 gttgtggact ccgatttatc acgaagaacc cgccgtagag atgcaagcgc tggacagcca
     2701 tgacttcgca tcgatcgggc ggctgaaacc gggagcaagt gaggctgaag gaagagcgga
     2761 gttagcggtc atcgtcaaac ggctgcacga ggaacaccg
//
LOCUS       DASTPM010000460         2952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_938864, whole genome shotgun sequence.
ACCESSION   DASTPM010000460 DASTPM010000000
VERSION     DASTPM010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938864"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..108
                     /gene="rpsI"
                     /locus_tag="VFR42_08150"
     CDS             7..108
                     /gene="rpsI"
                     /locus_tag="VFR42_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU0369164.1"
                     /translation="MRTNGFLTRDSRMKERKKYGQKGARKRFQFTKR"
     gene            325..1200
                     /gene="rpsB"
                     /locus_tag="VFR42_08155"
     CDS             325..1200
                     /gene="rpsB"
                     /locus_tag="VFR42_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0369165.1"
                     /translation="MAVEITMKELLEAGVHFGHQTRRWNPKMKEYIFGERNGIHIIDL
                     QKTLKMFREASRFVSEISSHGRTVLFVGTKRQAQEAVAEEAKKCGAFFVNHRWLGGTL
                     TNWSTLQKSIKRLKLLKAMNEDGRMAELSKKERARLDREMKHLFQNLEGIENMAQLPD
                     AMFVIDSNAEEIAVKEARRMGVPVVSVVDTNCDPDVVDWIIPGNDDALRAIRLFTSKI
                     SESVLEGHNLYQQSQVADQKAAADQALVEGVEYVDTSAYEQYEKQEGDFVEGEVPLEA
                     PEPAETTVPPDEESR"
     gene            1355..2002
                     /gene="tsf"
                     /locus_tag="VFR42_08160"
     CDS             1355..2002
                     /gene="tsf"
                     /locus_tag="VFR42_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0369166.1"
                     /translation="MSTQPQAIQVPAQLVKELRERTGAGFNDCRAALVEAGLDVEKAI
                     NIFRKKGQAAAAKKAQREASEGLVGHYIHAGGKIGVIVEVNCESDFVARTPAFQQLCH
                     DIAMHIAALDPRYLRREEVTSEMLEKERDIYREQAKATGKSEQVVEKIVTGKMEKFYE
                     ENCLYEQHYIKDESVTIGEMITQAVANLGENIAVRRFVRFKVGEVSGAATETASA"
     gene            complement(2123..>2952)
                     /locus_tag="VFR42_08165"
     CDS             complement(2123..>2952)
                     /locus_tag="VFR42_08165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0369167.1"
                     /translation="FADVTLIRPEAQATWRPALSKQRWDAADAEFAKAKPGKPGQWKF
                     RRALPARWEMQRNALRFWVQPAPSGHVGIFPDQACHWDWLAEQTRHSAGPAKVLSLFG
                     HTGLATLAATAAGQEVTHVDASRKAVALARENQELSGLADRPVRWVVEDALTFVQREA
                     RRGNRYHGLILDPPRFGRGPTGEIWKLKDSLPELLAACKAVLSPKPLFVLLNLYVTVV
                     TRDRAKQKTESLQKSLQKMLHNSPLEITSGELAVLDGAARRISASVFARAQRRPANS"
BASE COUNT          657 a          903 c          834 g          558 t
ORIGIN      
        1 ccctcgctgc gcaccaacgg ttttctgacg cgcgactccc gtatgaaaga acgcaaaaaa
       61 tacgggcaaa aaggcgcgcg caagcgcttc cagttcacga agcgttagtt ttctgtgggt
      121 cggctctact gtcatcctga ggctatccga taaggatagc cgaaggatct caaccgagcc
      181 cctctcacaa agcgacctcc gccggatggc ttcacctccc ttcggcgaga tcatggccgg
      241 caagctgccg gcaagaaggc tgctctcccg cgtatccgca atctcgatcc gcggccgcaa
      301 gcagctatcc ttaaaggagg aaacttggca gttgaaatta ctatgaagga gctgctggag
      361 gccggagttc actttggcca ccagacgcgc cgctggaacc ccaaaatgaa ggaatacatt
      421 ttcggggagc gcaacggcat ccacatcatc gatttgcaga aaactttgaa gatgttccgc
      481 gaagccagcc gcttcgtgag cgaaatttcc tcgcacggac gcaccgtcct gttcgtcggc
      541 accaagcgcc aggctcagga agccgtcgct gaagaagcca agaagtgcgg cgcttttttt
      601 gtgaatcatc gctggctggg cggcaccctc accaactggt ccaccctgca gaaatccatc
      661 aagcgcctga aacttctcaa agccatgaac gaagatggcc gcatggccga gctttccaag
      721 aaagagcgcg cgcgtctcga tcgcgaaatg aagcatttgt tccaaaatct tgaaggcatc
      781 gagaatatgg cgcagcttcc cgacgccatg ttcgtgatcg attccaacgc cgaagaaatc
      841 gcggtcaagg aagcgcgccg catgggcgtg ccggtagttt cggtggtcga caccaattgc
      901 gatcccgacg tggtcgactg gattattccc ggcaacgatg acgccttgcg agccattcgc
      961 ctgttcacct ccaagatttc cgaatccgtg ctggaaggcc acaatctcta ccagcaatcg
     1021 caggtcgccg accaaaaggc cgctgccgat caggctttgg tcgaaggcgt cgaatacgtt
     1081 gacaccagcg cctacgaaca gtacgaaaag caggaaggcg atttcgtcga aggcgaagtc
     1141 ccccttgaag cgcccgaacc ggccgagacc accgttccgc ccgacgagga atcccggtaa
     1201 gcaacgcgct ggcgaagttt tcaacaggcc cggcgctgca gtccaactcc agcgccgggc
     1261 cgttgtgcaa ataataatgt agcggccgat ctttaaatcg gccggcgagt tttgtaccac
     1321 cgggcgcgag cccgcaagcg agagaacaaa aataatgagc acacaaccgc aagccatcca
     1381 agtccccgcg cagctggtca aggaattgcg cgagcgtacc ggcgccggtt tcaacgattg
     1441 ccgcgccgca ctggtcgagg ccggcctcga cgtcgaaaag gccatcaaca tttttcgcaa
     1501 gaagggccag gccgcggcgg ccaagaaagc ccagcgcgaa gccagcgaag ggctggtcgg
     1561 ccactacatc cacgccggcg gaaaaatcgg cgtgatcgtg gaagtgaatt gcgaatccga
     1621 tttcgtcgcc cgcactcctg ctttccagca gttgtgccac gacatcgcca tgcacattgc
     1681 cgcgctggat ccgcgctacc tgcgccgcga ggaagtcacg tcggaaatgc tcgaaaaaga
     1741 gcgcgacatt tatcgcgagc aggccaaggc cactggcaaa tccgagcagg tggtcgaaaa
     1801 aatcgtcacc ggcaaaatgg aaaagttcta cgaggaaaat tgcctctacg agcagcacta
     1861 catcaaagac gagtcggtaa ccatcggcga aatgatcacc caggcggtgg ctaacctggg
     1921 cgaaaatatc gccgtccgtc ggttcgtaag attcaaagtc ggcgaagtaa gcggcgccgc
     1981 aacagaaacc gcctccgcat aattcagcag gcatatccat ccaatcgatc tgagagcgtc
     2041 ccgaaagggg cgctcttttt ttgcagcgag gattattcgc aagctgcgga tttttccact
     2101 cgatgccggc gccgcccgat ggtcaggaat tcgccggcct gcgctgcgcg cgcgcaaaca
     2161 ccgacgccga aattctgcgg gctgcgccgt ccagcacggc caattcgccg gaggttattt
     2221 ccagcggcga attgtgcagc attttctgca gtgacttttg caacgattcc gttttctgtt
     2281 ttgcgcggtc gcgggttact accgttacgt atagatttag cagcacgaac agcggcttgg
     2341 ggctcagcac cgctttgcat gcggctagca gttccggaag cgaatctttc agcttccaga
     2401 tttcgccggt tgggccgcgg ccgaatcttg ggggatcgag aatcagtccg tggtagcgat
     2461 tgccgcggcg cgcttcccgt tgcacaaaag ttagcgcgtc ttccaccacc cagcgcaccg
     2521 gccggtcggc gagcccggag agttcctgat tttcccgcgc gagcgctacc gctttgcgcg
     2581 aagcgtctac gtgggtgact tcctgcccgg ccgcggtcgc cgccagcgtc gccaggccgg
     2641 tatgcccgaa caaactcaac accttcgccg gacccgcgga gtggcgcgtt tgttccgcca
     2701 gccaatccca atggcaggcc tgatccggga agatccccac gtgtccggaa ggcgccggct
     2761 gcacccagaa acgcagcgcg ttccgctgca tctcccatcg cgcgggaagc gcgcggcgaa
     2821 atttccattg gcctggcttt cctggctttg cttttgcaaa ttccgcgtcg gccgcgtccc
     2881 atctttgttt ggacagcgct ggccgccacg ttgcttgggc ctccggccgt atcaaggtga
     2941 catccgcaaa tc
//
LOCUS       DASTPM010000461         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1429237, whole genome shotgun sequence.
ACCESSION   DASTPM010000461 DASTPM010000000
VERSION     DASTPM010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429237"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1090)
                     /locus_tag="VFR42_08170"
     CDS             complement(74..1090)
                     /locus_tag="VFR42_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369168.1"
                     /translation="MPLFNLAIVGFGNVGRALLRLLIAKETELRRRYDVRWRLTGVAT
                     RRAGWVADADGLNPLALLNGHWPQRAHLEVLSNVTDWLDRSRADVFFETSSLDPQSGQ
                     PATDHLRAALHHGAHAISANKGPVVHAYAELMSLARESGKSFLCESTVMDGVPIFSLF
                     PTGLPAVELRSFSGVLNSTTNVVLTEVEKGLSFEEAIRRAQALGVAETDPSNDLDGWD
                     AAVKVVALTTVLMGIPVKLDQVQRTGLRDLSAEKIRSVRAAGMRYKLVCRAERRGTGV
                     ECMVRPELLLASDPLANLDGTSSAVRFDLDVFSFSIVEHNPGIEATAYGLLADFLRAV
                     RPSA"
     gene            complement(1112..3394)
                     /locus_tag="VFR42_08175"
     CDS             complement(1112..3394)
                     /locus_tag="VFR42_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEU0369169.1"
                     /translation="MRRALILAVVAAAVLFLANHFTTADEPLLAGFSADSSRAERQWE
                     EKFKAIPSPQLMRDYMQRLSARPHNVGTPYDKDNAEWLLAKFKEFGLDAHIETFEILF
                     PTPKERAVELVEGGPNFTAKLQEPAVAQDPTSNQQSEQLPTYNAYSIDGDVTAPLVFV
                     NYGIPEDYEQLARLGVSVKGAIVIAKYGHSWRGIKPKVAAEHGAVGCLIYSDPNEDGF
                     KAGETFPAGPYRPRDGVQRGSVADDPIYPGDPLTPGVGATKDAKRLDLKDVAVLTKIP
                     VLPISYGDAEPLLAAISGQIAPRAWRGGLGLTYHVGPGPAKVHLKVKSDWGLKTIYDV
                     IGKIPGTTAAGEWIIRGNHHDAWVNGAEDPISGLIAVMEEARAMGELVKQGWKPKRTI
                     IFCAWDAEEPGLIGSTEWAEQHADELKQHAVVYINSDVSTRGYFGAEGSHTLEKFIND
                     VARDVQDPETKLSVWKRAQLRQIGRPDDISERQEARSRSDLRIGALGSGSDYGAFLQH
                     DGVAALNLGFGGEDGGGIYHSIYDDFYWYTHFADTDFVYGRALAQTAGTAVMRLANAD
                     LLPFEYTNFADTMQTYVKELKTLAQKSREEISERNRELDEGVFAAMTDPRVKSVPPPR
                     EAVPPFLNFAPLDNATEALNRAAAEYRKALDKAVANGGAALAKASLTEVNAALMASEH
                     QLTLEQGLPGRPWFKHFVDAPGQYTGYEAKTLPAVREPLEQKQWKDVDAQIVVTARVL
                     EAEAAHITSVAQKLDAAVGK"
     gene            complement(3493..3837)
                     /locus_tag="VFR42_08180"
     CDS             complement(3493..3837)
                     /locus_tag="VFR42_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369170.1"
                     /translation="MPKFKKHIFICMNQREAGHPRGCCDPTGQGELQRLFKVKLTQRG
                     IQLVRANKSGCLDQCELGPTVAVYPEGIFYGAVRPEDVDEIIESHILNDKPVERLRIP
                     DEILNTVHKKPN"
BASE COUNT          819 a         1108 c         1369 g          868 t
ORIGIN      
        1 ggatgcaccg gtgcgggatg agcggtgtga tgcggggatt gcgacgccgc cttacgccga
       61 aaactttcga agtttaagct gagggccgaa ctgcgcggag aaagtcagca agcaggccat
      121 aagcggtagc ttcgatgcca ggattgtgtt cgacgatcga gaagctgaaa acgtcgaggt
      181 cgaaacggac ggcggagctg gtgccgtcga gattggccag cggatcgctg gcgagcagaa
      241 gttcgggacg gaccatgcac tcgacgccgg tgccgcggcg ttcagcgcgg cagacgagtt
      301 tgtagcgcat gccggcggcg cgcacgctgc gaattttttc ggcgctgagg tcgcgcaagc
      361 cggtgcgctg aacctggtcg agtttgacgg gaatccccat gagcacggta gtgagcgcaa
      421 cgacttttac ggcggcgtcc cagccgtcga ggtcgttgct aggatcggtt tccgcgaccc
      481 cgagagcctg agcgcggcga atggcttctt cgaaggacaa ccctttttcg acttcggtga
      541 ggaccacgtt ggtggtggag tttagaacgc cggaaaaact gcgcagttcc accgcgggca
      601 agccagtggg aaacaaagaa aagattggca cgccatccat cacggtggat tcacagagaa
      661 aacttttgcc gctttcgcgc gcgagggaca tgagttcggc gtacgcgtgc accaccgggc
      721 ccttgttggc ggaaatagcg tgcgcgccat ggtgtagcgc agcccgcaga tgatcggttg
      781 cgggctggcc gctttgcgga tcgagtgaac tggtttcgaa aaacacgtcc gcgcgggagc
      841 ggtcgagcca atcggtgacg ttggacaaaa cttccagatg cgcgcgctgc ggccaatgcc
      901 cattgagcag cgcgagggga ttgagaccat cggcatcggc cacccagccg gcacggcgcg
      961 tggctacacc ggtcaggcgc cagcggacat cgtaacggcg gcgcagctcc gtttccttgg
     1021 cgatcagcag gcggagcagc gcgcggccaa cgttgccgaa accgacgatg gcgaggttga
     1081 agagaggaat ggcggcctcg tttccggtgg attattttcc tactgcggcg tcgagctttt
     1141 gcgcgacgga agtgatgtgc gcggcttcgg cttcgagcac gcgcgcggtg accacgattt
     1201 gcgcgtccac atctttccac tgtttttgtt caaggggctc gcgcacggcg ggcaaagttt
     1261 tcgcttcgta gccggtgtac tggccgggcg cgtcaacgaa atgtttgaac cacgggcggc
     1321 cgggcagtcc ttgttccagc gtgagctggt gctcgctggc catcaatgcg gcatttactt
     1381 cggtgagaga ggccttcgcc agagcggcgc cgccattggc gacggctttg tcgagtgcct
     1441 tacggtactc ggctgcggct cgattgagag cttcggtggc gttatcgagc ggcgcgaaat
     1501 tcagaaatgg cggaacggct tcacgcggcg gtggaacaga cttgacgcgc ggatcggtca
     1561 tggcggcaaa aacgccttcg tcgagctcgc gattgcgctc gctgatttct tcacgcgatt
     1621 tctgcgccag cgttttcagc tctttgacgt aagtctgcat ggtgtcggcg aaattcgtgt
     1681 attcgaacgg caggaggtcg gcattggcca gccgcataac agcggttccg gcagtttgcg
     1741 ccagggcgcg accataaacg aagtcggtgt cggcaaaatg cgtgtaccag taaaagtcgt
     1801 cgtagatcga atggtagatg ccgccgccat cttcgccgcc gaaaccgaga ttgagcgcgg
     1861 cgacgccgtc atgctgcagg aatgcgccat agtcggaacc tgaacctagc gcaccgatgc
     1921 gcagatcgga ccgggagcga gcttcctggc gttcggaaat gtcatcgggg cggccgatct
     1981 ggcgaagttg cgcgcgcttc cagaccgaga gcttggtttc gggatcctga acgtcgcgtg
     2041 ccacgtcgtt gatgaatttt tccaatgtgt gcgaaccttc ggcgccaaaa taacctcgtg
     2101 tgcttacgtc ggaattgatg tagacgaccg cgtgctgttt caattcgtcg gcgtgctgtt
     2161 cggcccattc ggtggagccg atgaggccgg gctcttccgc atcccacgca caaaaaatta
     2221 tggttcgctt gggcttccac ccctgcttga cgagttcgcc catggcgcgc gcttcttcca
     2281 tgacggcgat gagcccggag atgggatctt ccgcgccgtt tacccaagca tcgtggtgat
     2341 tgccgcggat gatccactca ccggcagcgg tagtgccggg aatcttgccg atgacgtcgt
     2401 aaatcgtctt caagccccag tcggatttca ctttcaggtg aactttggcc gggccgggac
     2461 ccacgtgata cgtgagacca agtccgccac gccacgcgcg gggagcgatc tggcctgaga
     2521 tagcggccag caaaggctca gcgtcgccgt aggaaatcgg tagcacggga attttggtca
     2581 agacggcaac gtccttcaag tcgaggcgct tggcgtcctt ggtggctccg acgcctggtg
     2641 tgagcggatc gccgggataa atgggatcgt cggcgacgct gccgcgctgt acgccgtcgc
     2701 gcgggcgata cggcccggca ggaaaagttt cgccggcctt gaatccgtct tcgttgggat
     2761 cggaatagat gaggcagccg acggcgccgt gttccgcggc aactttgggc ttgatgccgc
     2821 gccaggagtg gccgtacttg gcgatcacga ttgcgccctt caccgagacg ccgagacggg
     2881 cgagctgttc gtaatcttcg gggatgccgt agttgacgaa gactagcggc gcggtgacgt
     2941 cgccgtcgat ggagtaggcg ttgtaggtgg ggagctgctc actttgttga ttggacgtgg
     3001 gatcttgcgc gacggcgggt tcctgaagtt tggcggtgaa gttcggaccg ccttcgacga
     3061 gttcgacggc gcgttctttt ggagtgggaa acaagatttc aaatgtttcg atgtgggcgt
     3121 cgaggccgaa ctccttgaat tttgccagta gccattcggc gttgtctttg tcgtaaggcg
     3181 tgccgacgtt atgcggccgc gccgagagtc gctgcatgta gtcgcgcatg agctgcggac
     3241 taggaatggc tttgaatttt tcttcccact ggcgctcggc gcgcgaagaa tcggcggaga
     3301 agccggcaag taacggctca tcggccgtgg tgaagtgatt ggcgagaaat agaacagctg
     3361 cagctacgac agccagaata agtgctcgac gcatgataaa tggcacgatg ccccctgaac
     3421 gaacaggtat tttgcacgaa ctaagcggat tgcaaaaggg cctgagagga aaaatccggc
     3481 ggcgagattt gttcagttgg gctttttgtg cacagtgttc aggatttcgt cgggaatgcg
     3541 caagcgttcg acgggcttgt cattcaggat gtgcgattcg atgatttcgt cgacgtcttc
     3601 gggccggaca gcgccgtaaa aaatgccctc agggtagacg gcgacggtgg ggccgagctc
     3661 acactgatcg aggcagccgg atttattggc gcgaacgagt tggatgccgc gctgcgtaag
     3721 tttcacttta aagagccgct gtaattcgcc ctgcccggtg ggatcgcagc agccgcgcgg
     3781 gtgaccggcc tcgcgctgat tcatgcaaat gaaaatgtgt ttcttgaact ttggcatcta
     3841 ggcttccact tccaagatac gtggaaaagc cgatgctaca caaaccgtgc tgcggctcga
     3901 acacctggcg acttcgccta cgctaaagct tttcctgggg agataggcat tacgtttccc
     3961 aagctggcag acgagaagcg cgaggcgcac cgggcgatgc aggtaaagag cacaaatttg
     4021 ttccactttt gcgcaaaggc aatttcgaag cgcgcgctcg agccaaggct gcggggacat
     4081 tcgcagtaca gcctgggcaa gaatccgttt caaccaagcc gaaattttgt ttttgggccg
     4141 ggcgatgtgg atcgcttcgc acca
//
LOCUS       DASTPM010000462         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1429273, whole genome shotgun sequence.
ACCESSION   DASTPM010000462 DASTPM010000000
VERSION     DASTPM010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429273"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="VFR42_08185"
     CDS             <1..1394
                     /locus_tag="VFR42_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU0369171.1"
                     /translation="PKYSLKYYVGLAKQLEKLGVHFLAIKDMAGLCKPYAAFELVKKL
                     KEEVALPIHFHTHDTSGLNAATVLKAADAGADIADGAVAAMSGGTSQPNLNAVVAALK
                     NTLRDTGLDLAALNDCSDYWETVRSYYLPFDSGPKAGSARLYEHEIPGGQYTNLREQA
                     VAMGLGHRWREVERMYAEVNQLFGDIVKVTPSSKVVGDMTLFLMAKEMTVSDVLKLDE
                     HHDVAFPNSVVEMFSGVLGTPPDGWPRKVQKILLRGAKPMKGRPSAAMPAAEFAAEKE
                     SLERKAGHAIDEADLLSYLLYPEVFLKFDKFRQAHGDVSVLPTPPFFYGLKSGEEIMV
                     SIEAGKTLIIKYMTASDPHPDGTRTLFFELNGQPREVNVRDKSLRVVERAHPKADAAD
                     PGQVGAPTAGLISGIAVQANHTVERGAKLMTLEAMKMQSNIYAPIAGRVVKLLVAPGQ
                     SVEAKDLLVTIAP"
     gene            1408..2181
                     /locus_tag="VFR42_08190"
     CDS             1408..2181
                     /locus_tag="VFR42_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU0369172.1"
                     /translation="MSDGQLRRHDSFRSRFLRNQRSLVVYLPPGYEELPQRRFPVLYL
                     HDGQNLFDGSTSFIQGQDWHVGQTADAEIHGGRVEPLIIVGIYNTGKARIREYTPTKA
                     PKLGGGRADRYGRFLLEEVMPFVHREYRAEGGPDRTGIGGSSLGGLVSLYLGLTRSDV
                     FGKIAALSPSVWWHYRVIHTFAERMAVHARPRIWLDIGTREGPRIVEDVERYRDILLS
                     RGWRYDVDLHYERIEGAEHNEAAWAKRVGPFLRFLYPAR"
     gene            2228..>2719
                     /locus_tag="VFR42_08195"
     CDS             2228..>2719
                     /locus_tag="VFR42_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369173.1"
                     /translation="MPEQRPLAILCVSTYEKGQEFIRTCREIGCTVYLLTVEKLRNAD
                     WPREAIDEMFFMPEELPLPQLLNAVSYAARTRKIDRIVALDEFDMENVSAIREHLRIP
                     GMGLTTVRYFRDKLAMRVRAKEVGINVPEFVHVLNHDELREFMARVPGPWLLKPRSQA
                     SGIG"
BASE COUNT          600 a          672 c          906 g          541 t
ORIGIN      
        1 ggccgaaata ttcgctgaaa tattatgtcg ggctggcaaa acagctggaa aagctgggcg
       61 tgcattttct ggcgatcaag gacatggcag ggttgtgcaa gccgtatgcc gcgttcgaac
      121 tggtaaaaaa gctgaaagaa gaggtggcgc tgccgattca ctttcacacg catgacacga
      181 gtgggttgaa cgcggcaaca gtgttgaagg cggcggacgc aggggcggat atcgcggacg
      241 gcgcggtggc ggcgatgagc ggcggaacca gccagccgaa tctgaacgca gtggttgctg
      301 cgttgaaaaa cacgttacgg gataccgggc tcgatctcgc ggcgttgaat gattgctcgg
      361 attactggga gacggtgcgg tcatattact tgccgtttga ttcgggaccg aaggcgggga
      421 gcgcgcgcct gtacgaacac gaaattccgg gcggacaata cacgaacttg cgcgaacaag
      481 cagtggcaat gggcttgggg caccgctggc gcgaagtcga acggatgtat gccgaggtga
      541 atcagctatt cggcgacatt gtgaaggtga cgccatcgag caaagtggtt ggcgacatga
      601 cgctgttttt gatggcgaaa gagatgacgg tgtcggatgt cttgaagctg gacgagcacc
      661 acgatgtggc gtttccgaat tcggtggtgg agatgttttc gggcgtgctg ggcacgccgc
      721 cggacggctg gccgcgtaaa gtacagaaga ttctgctacg tggagcgaag ccgatgaagg
      781 gtcggccgag cgcagcgatg cccgcggcgg aatttgcggc cgagaaagaa tcgctggaga
      841 gaaaggcagg ccacgcgatt gacgaggcgg acctgctgag ttacctgctg tatcccgaag
      901 tgtttttgaa gttcgataaa ttccggcaag cgcatggcga tgtgagcgtg ctgccaacgc
      961 cgccgttctt ttatggattg aaatcgggcg aggagattat ggtttcgatc gaggcgggca
     1021 aaacgctgat cattaaatac atgacggcga gcgatccaca tccggacggc acacggacac
     1081 tgttttttga gttgaacggg cagccgcgcg aagtgaacgt gcgggacaaa tccttgcggg
     1141 tggtggagcg ggcgcatccc aaggccgatg cggcggatcc gggacaagtg ggcgctccga
     1201 cggccgggct aatcagcggt atcgcggtgc aagcgaacca caccgttgaa cgtggtgcta
     1261 aactgatgac gctcgaagcc atgaaaatgc aatcgaacat ttatgcgcca atcgcaggtc
     1321 gcgtagtgaa gctactggta gcgccgggac aaagtgtgga agcgaaagac ctgctcgtga
     1381 ccatagcccc gtgagcctga acgccccgtg agtgacggac aactccgcag acacgacagt
     1441 ttccgatcta ggttcctgag aaatcagagg agcctggtgg tgtatctgcc tccgggttat
     1501 gaagagctgc cgcaacggcg ctttcctgtt ttgtatctgc atgacggaca aaatcttttc
     1561 gatggctcga cttcgttcat tcaggggcag gattggcacg ttggccagac cgccgatgcc
     1621 gaaatccatg ggggccgtgt cgagccgctg atcattgttg gcatctacaa cacggggaaa
     1681 gcccgcattc gggaatacac gccgaccaaa gcgccaaagc tcggcggagg acgcgcggac
     1741 cgttacggaa gattcttact ggaagaagtg atgccatttg tgcaccgcga atatcgcgcg
     1801 gaaggggggc cggaccggac agggatcggc gggtcgtcgc tgggcggcct ggtgtcgctt
     1861 tacctgggat tgacgcgctc ggacgtgttc ggaaaaattg cggcgctgtc gccgtcggtg
     1921 tggtggcact accgtgtgat ccacactttt gcggaaagaa tggcagttca tgcgcgaccg
     1981 cgcatctggc tggacatcgg cacgcgcgaa gggccacgga tcgtggaaga cgtcgaacgc
     2041 tatcgcgata ttctactttc gcgcggctgg cgttacgacg tagacctgca ctatgagcgc
     2101 atcgaaggcg cggagcacaa cgaagcggcg tgggctaagc gggttggtcc ttttctgcgg
     2161 tttttgtatc cggcccgata gttgcgcggt atacttcgcc gctcacctgt agtagaggag
     2221 gcttggcgtg ccagagcagc gacctctcgc aatcctctgc gtcagcacct acgaaaaagg
     2281 gcaggagttt atccgaacgt gcagagagat cggctgcacg gtgtatttgc ttactgtaga
     2341 aaaactgcgt aacgcggact ggccacgcga agctatcgac gaaatgttct tcatgccgga
     2401 ggaattgccg ctgccgcaac tgcttaacgc ggtaagttat gcggcccgca ccagaaagat
     2461 cgaccgcatc gttgcactcg acgagttcga catggagaac gtgtcggcga ttcgcgagca
     2521 cttgcgcatt ccgggcatgg gcctgacgac ggtccggtat tttcgcgaca aactggcgat
     2581 gcgagtgcgg gccaaggaag tcggcatcaa cgtaccggaa ttcgtgcacg tgctgaatca
     2641 tgatgagttg cgggagttca tggcacgggt gcccgggccg tggttgttga aaccgcgttc
     2701 gcaagcttcg ggaatcggc
//
LOCUS       DASTPM010000463         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_564508, whole genome shotgun sequence.
ACCESSION   DASTPM010000463 DASTPM010000000
VERSION     DASTPM010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564508"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2659)
                     /locus_tag="VFR42_08200"
     CDS             complement(<1..2659)
                     /locus_tag="VFR42_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369174.1"
                     /translation="MEAWEFAAKALSGNPYDIPYAVLYRLDRELTSAEAVAFAGISPG
                     DAFLQDLVNLKSETQVASLIRQALQQTSPVEFGDLPALGVALPGGFWGGNSSDLILLP
                     IAQAGQESGMGVLIAGVNPHKKLDEDYRGFLNLVASQIAKSVADARAVDEERRRAESL
                     AALDRAKTTFFSNISHELRTPLTLILGPLEELRSRGMDPTNVSLGEIELMHRNGTRLL
                     KLVNTLLEFSRIEAGRIQATYEQVVLADSTADIASVFRSAIEKAGLRFDVHCDEPSAQ
                     MYVDKQMWEKIVLNLLSNALKFTHNGSIAVDLREIEGGAELRVSDTGVGIPEDEMPRL
                     FERFHRMERTQGRTNEGTGIGLALVQELVKLHGGTIRAQSAVGQGTQFIVFIPSGSAH
                     LPPDRIANSTKAVVPKTNREPFVEEAMAWFPDASVLPANQTGAIATGRAATHSGVNPL
                     VLLVEDNRDMRHYLTRLLNEEGYSVDAASDGLMALKRLESIRPDLIVSDVMMPRLDGV
                     GLLRRLREDDALSSIPFILLSARADEDFRLEGLEAGADDYLIKPFHARELLSRIKLQL
                     ELARRKRTSQSRQSATAAEAILGTWDVNLHTNEAFWSPGLFSLLGYAPNDCTPSWDAL
                     LARVLPADQLAVREQWASAIADRRDYRFQFRILLPDGQTRWVQAKGRFYFDDTGKPYR
                     DLGSLQDISAQKRNSEDPQGAVAEPKTLEQEERKALQDRLTEVASQAELLDLVSDAAL
                     ICDTAHRITYWNRAAERLYGWSSEEAVGKDVSDMLQTEYPVPFADLLATLEEQGRWEG
                     ELVHTNRYGMRVLVYSRWIHKRGERGEFKGWLEINADLTQQRQAEETARRLSGRILQL
                     QDEERRKIARELHDSVGQYLAMLKITIDRG"
BASE COUNT          531 a          772 c          867 g          581 t
ORIGIN      
        1 cgccgcggtc aatggtgatc ttcagcatgg cgagatactg gccgacgctg tcgtgcagct
       61 cgcgcgcgat ttttctgcgc tcctcgtcct gcagctgcag aatgcgtccg ctcaggcggc
      121 gcgcggtttc ttcggcctgg cgctgctgcg tcaggtcggc attaatttcg agccagcctt
      181 tgaattcgcc gcgttcgccg cgtttgtgaa tccagcggct gtaaaccagg acgcgcatgc
      241 catagcggtt ggtgtggaca agttcacctt cccaacggcc ctgttcttcc agagtggcga
      301 gaaggtccgc gaagggaact ggatactcgg tctgaagcat atcggaaaca tctttgccga
      361 cggcttcctc ggaactccac ccgtagaggc gttcggcggc ccggttccag taggtaatcc
      421 gatgggcggt gtcgcagatc aacgcagcgt cgctgacaag atcgagcaac tccgcctgcg
      481 aagcgacctc ggtgagacgg tcctgcaaag ctttgcgttc ttcctgttcg agcgttttgg
      541 gttcggcgac ggcgccttga gggtcctctg agttacgctt ttgggcgctg atgtcttgca
      601 gcgagcccag gtcgcggtag ggcttgccgg tatcgtcaaa ataaaaacgc cctttggctt
      661 gcacccagcg ggtttgtcca tccggcagca ggatgcgaaa ctggaaacgg tagtcgcggc
      721 ggtcggcgat ggccgaggcc cactgctcgc gaacagcaag ttggtccgcg ggaagtacgc
      781 gggccaacaa ggcatcccac gaaggagtgc aatcgttggg ggcgtagcct aacaacgaaa
      841 aaaggccggg cgaccagaag gcttcattgg tgtgcaggtt aacgtcccag gttcccaata
      901 tcgcctcagc cgctgtggcg ctttggcgac tttgggaagt gcgttttcgc cgcgcgagtt
      961 ccagttgcag cttgatacgc gacagcagtt cgcgagcatg gaacggcttg atgaggtagt
     1021 cgtcagcccc ggcttccagt ccttccaaac ggaaatcttc gtcagctcga gcggacaaga
     1081 gaatgaaggg gatgctgctc aatgcgtcgt cttcgcgcag gcgacgcagc aagccgacgc
     1141 cgtccaagcg gggcatcatg acatcgctca cgatgaggtc ggggcggatg gactcaaggc
     1201 gtttgagggc catgagtccg tcggaggcgg cgtccaccga atagccctcc tcgttgagca
     1261 ggcgcgtcag ataatggcgc atgtcgcggt tatcttccac gagcagaacc agcgggttga
     1321 ccccggagtg cgtagcagcg cggccagtgg cgatggcgcc agtttggttg gcgggaagga
     1381 cggaagcgtc cggaaaccat gccatggctt cttcaacgaa aggctcgcga ttggttttgg
     1441 gaaccacggc tttcgtgctg tttgcaatgc ggtctggcgg caagtgagcg gagccggagg
     1501 gaatgaaaac gataaactga gtaccttggc ccaccgcgct ctgcgcccga attgtaccgc
     1561 cgtgaagttt caccaattct tgtaccagag ccaggccgat gcctgtgcct tcattagtgc
     1621 ggccctgcgt tcgttccatg cgatgaaacc gctcgaacag gcgaggcatt tcgtcttccg
     1681 ggatgccgac gccggtgtcc gaaacgcgaa gctccgcgcc gccctcgatt tcgcgcaagt
     1741 ctacggcgat gcttccgtta tgcgtgaact tcagcgcgtt ggacaataaa ttgaggacaa
     1801 tcttttccca catttgtttg tcgacgtaca tttgagcgct cggctcgtcg cagtgcacat
     1861 caaatcgcag accggccttc tcgatggccg agcggaagac gctggcaatg tcggcggtag
     1921 aatcggcgag aaccacctgt tcgtaggtag cctgaatgcg gcccgcctca atgcgggaaa
     1981 attccagcag cgtgttaacc agcttcagga ggcgcgtgcc atttctgtgc atgagttcaa
     2041 tttcgccaag gctgacgtta gtgggatcca ttcctcgcga gcgcaactct tcgagcggac
     2101 ctagaattaa agtgagtggg gtgcgcaatt catggctgat gttgctgaag aacgtggttt
     2161 tggcgcgatc caacgcggcg agagattcgg cgcggcggcg ctcttcgtca accgcccgcg
     2221 catccgccac gctttttgcg atttggctgg ccacgaggtt taggaatcct cggtaatcct
     2281 catcgagttt cttatgggga tttacgccgg cgatcagaac gcccattccc gactcctgac
     2341 cggcttgtgc gatgggaagg agaatcaggt ccgaggagtt tcccccccag aaaccgccgg
     2401 gtagagcgac gccaagggct ggcaaatcac cgaattcgac ggggctcgtt tgttgcaggg
     2461 cttgtcgaat caacgaagcg acttgggttt cgctcttcag gttcaccaag tcctgtagga
     2521 aggcatctcc cggagaaatt cccgcgaagg ccacggcttc cgccgaggtg agctcgcgat
     2581 ccaaccgata caggaccgcg taggggatgt cgtaggggtt gccgctgagg gccttggcgg
     2641 cgaactccca ggcctccatc tcggttttcg cgtcgacact gcgtgaagcc aggtcccgga
     2701 gagtgagcgg tcggcgctgg ctaatcacct cggcagtggt ttcggcgacc g
//
LOCUS       DASTPM010000464         6903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1284929, whole genome shotgun sequence.
ACCESSION   DASTPM010000464 DASTPM010000000
VERSION     DASTPM010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284929"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VFR42_08205"
     CDS             complement(<1..865)
                     /locus_tag="VFR42_08205"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEU0369175.1"
                     /translation="MSELAKPANDEAQARRAVEEAAQRSYGKLVAFLAARTRDVAAAE
                     DALSEAFASALVDWPLHGCPENPEGWLLTVARRKSIDAMRRRQRDEAAGADLQLITEG
                     LEAAAASTQIPDQRLALLFACAHPAIEEGVRAPLMLQVVLGLDAGAIASAFLISPATM
                     GQRLVRAKNKIRQAGIPFRIPEQEELRGRLDTVLDAIYAAFAEGWSDAAGADAARRDL
                     AQEAIFLCRVVIELMPEEPEALGLLALMLHAEARSRARRSAQGEYVPLAEQTVTCWDS
                     RMIREAEEVLLR"
     gene            complement(870..1427)
                     /locus_tag="VFR42_08210"
     CDS             complement(870..1427)
                     /locus_tag="VFR42_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0369176.1"
                     /translation="MRKLMVFNSMSLDGFIADGKGDMSWAHKQDEEWNSFVAGNASGD
                     GVLVFGRRTYDMMAGYWPTPMASQNSTVVAKRMNELRKIVFSHTMEKASWQNTTLMKG
                     ELAVEMKRLKGQPGADMVILGSASIVAQLSDARLIDEYQVAMNPVVLGRGKSMFAGLR
                     EKLPLKLVRSQSFQNGNVFLTYQPV"
     gene            complement(1487..1876)
                     /locus_tag="VFR42_08215"
     CDS             complement(1487..1876)
                     /locus_tag="VFR42_08215"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0369177.1"
                     /translation="MKYMLLDYVHESGWPQLTREEQAHWLGVYRAYMEAMTKAGVLKS
                     SGGLQGTPTATTVRIRNEKTQVLDGPYADAKEQLGGFHIIEVPDLDAAISWAARSPTA
                     LHGVVEVRPMHEGTFLTEDISKYLKPE"
     gene            complement(1942..2286)
                     /locus_tag="VFR42_08220"
     CDS             complement(1942..2286)
                     /locus_tag="VFR42_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0369178.1"
                     /translation="MDYMLMIYVNEAGWPKLSQAQQEQGMAAYTAYTEAMQKAGVFKS
                     GGRLYPSASAATVRAADGKPSVLDGPYADAKEQLGGFYIVDVTDRQAALSWAQRCPGA
                     GHGAVEVRQIWR"
     gene            complement(2408..2695)
                     /gene="zapA"
                     /locus_tag="VFR42_08225"
     CDS             complement(2408..2695)
                     /gene="zapA"
                     /locus_tag="VFR42_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU0369179.1"
                     /translation="MPTTRVQIFGQTYAIAGDLEPAYVQKLAVYVDEKMRAIADATTT
                     IDTQKAAVLAALAIADELHSGLRDRTEREELLREQAERCLTLVERALKQTA"
     gene            complement(2799..3524)
                     /locus_tag="VFR42_08230"
     CDS             complement(2799..3524)
                     /locus_tag="VFR42_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0369180.1"
                     /translation="MKRLVLVVFVALAVSASLPAQQASFSSFDRTPQPSFVAPAAAAA
                     DSTGALVPSLAIPAAAAPAALLSPVPSAALPDAPRAPQFGRGGDIGYRWDLAVGYEYV
                     HFESKPFSANLSGIHTSIAYSLNEWFALEGSLVSAFGGDVFQGGEMSKYVLFTGGGRI
                     LWNRQPHRLSPWAHVLAGMTHVNPQIANSSKNAFALQAGGGVDWFFNPRLSFRGEADY
                     VHTLLYSDSQNNFQIGVGVVLHF"
     gene            complement(3645..5381)
                     /locus_tag="VFR42_08235"
     CDS             complement(3645..5381)
                     /locus_tag="VFR42_08235"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0369181.1"
                     /translation="MPETAQHLLGQLVDGRFPLRRLLGVSPNSAVFLTDLPNQRPSEP
                     APEAAIKLIPEDPETAEQQLARWKSVAALSHPGLLRILHFGRCAMDGSPCLFVVTDLA
                     SENLGELLRQRALSPEEASGMMAPVLATLDFLHEKGLVHGGVKPSNILAIRDTIKLSP
                     DRILPAGESSTSWPMAAPYAPPESLLFPASDVWSLGISLYETLTQYLPNHDSSGHYVL
                     PQLPSPFAEVIRGALVQDATERISLDEIRSALDPSFVAKPKPVRAPVPVEASVKSPAE
                     PPTVAASASAAQEPETVPVPQPLAAAAQASAGTATARTTPAAPMPLQTVDPLAVPLSP
                     VSPTETRAKAPSHVPVSSLPNVNVTIAAPRRVPEVRASSGSLKYFLVGAAGTLVLAVL
                     IVPRMLRNSADSSVTSKTAAASSPATKPAAESTPASAAKSAPSTQESTTPSASKTAPA
                     SKPPAVAPPSTAVSPAENQAPVAPSVSSSRSTTPAVLGAAQVVHRVVPDVSQKARSTI
                     RGTVRINVRVQLNPDGSVSAAALDSGAGSQFFANLALKAARDWRFAPSGNTSALVRFD
                     FTNSGSTAYVVP"
     gene            complement(5647..6849)
                     /locus_tag="VFR42_08240"
     CDS             complement(5647..6849)
                     /locus_tag="VFR42_08240"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU0369182.1"
                     /translation="MANVSTKKGIIGILTGGGDVPGLNPAIRGVTYRALREGYQVIGI
                     RHGWGGLIDIVRDKDYDNSNNYQLLTEDVVNRASRTGGTFLHSSRTNPGKTPKGSVPE
                     HLRSQFTEEKNDLTAEVLKNLEWLGIDYLVPIGGDDTLSYGVRLHKEGFHVVAIPKTM
                     DNDVPGTDYCIGFSTCVSRTIQLANSIRTSAGSHERFLILEVFGRYAGYTAMLPTLAG
                     AADRCVIPEHKFNIDRLTELLVSDRIKNPSRYSVALVSEGAMFEGGEMIFEGESADAY
                     GHKKLGGIGDLVAEEVKNRSAKFNKGGKKIEVISQKLGYLVRSGEPDAIDSIVPMAYG
                     NLALDLILNKTFGHLVVFREVRYSHLPITTVTATKKFVDVNDQYDTERLRPKYNSFSN
                     KPLFLMGS"
BASE COUNT         1416 a         2202 c         1910 g         1375 t
ORIGIN      
        1 cccgtaacaa aacttcttcg gcctcgcgaa tcatccgcga atcccagcac gtcaccgttt
       61 gctcagccag cggcacatat tcgccctgcg cgctgcgccg cgctcggctg cgcgcttccg
      121 catgcagcat cagcgccagc agacccagcg cttccggttc ttccggcatg agctcgatca
      181 ctaccctgca cagaaaaatt gcttcctgcg ccaggtcacg tcgcgccgca tccgctccag
      241 ccgcatcgct ccagccttca gcgaacgccg catagatcgc atccagcacc gtatccaacc
      301 ggccgcgcag ctcttcctgt tcgggaatcc ggaacggaat ccccgcctgg cgaattttgt
      361 tttttgcgcg aaccagccgc tgccccatcg ttgccggaga aatcagaaac gccgatgcaa
      421 tcgcccccgc atccagtcct agcaccacct gcaacatcag cggcgcgcga actccctctt
      481 caatcgccgg atgcgcgcac gcaaacagca gcgccagccg ctggtccgga atctgcgtgc
      541 tggccgccgc ggcctccagt ccttccgtta tcagctgcaa atcggcgcct gcggcctcgt
      601 cgcgctgccg tcggcgcatc gcatcaatgc tctttcgcct tgccacggtc agcagccatc
      661 cttccggatt ctccggacaa ccatgcaacg gccaatccac cagcgctgat gcaaatgctt
      721 ccgatagcgc atcttcggct gccgccacat cgcgcgttcg cgccgccagg aatgccacca
      781 gtttgccgta gcttcgctgg gcagcttctt ccactgcgcg ccgcgcctgc gcttcatcat
      841 tcgctggttt tgccaattcg ctcaatgttt cacactggct gataggtcag gaacacattt
      901 ccattctgaa agctttgtga gcgaaccaac ttcaagggca acttctctct caagccagcg
      961 aacatggact tgccacgtcc caaaacaacc gggttcatag cgacctgata ttcgtcaatc
     1021 agccgcgcat cggaaagctg cgccacgatg ctcgcgctgc cgaggatcac catgtccgct
     1081 cccggctgcc ctttcaatcg tttcatctcg acggccaact cgcccttcat cagagtggtg
     1141 ttttgccagg acgctttctc catcgtgtgc gaaaacacaa tcttcctcag ctcattcatg
     1201 cgcttcgcca ccaccgtaga attctggctg gccatcggag tcggccagta tcctgccatc
     1261 atgtcgtagg tcctgcgtcc aaacaccaac accccgtcgc cgctggcgtt cccggccaca
     1321 aacgaattcc actcttcgtc ctgcttgtgc gcccagctca tatctccctt accgtcggcg
     1381 atgaatccat cgagcgacat cgaattaaat accatcaatt tgcgcatact actccccctg
     1441 cttgtcagcg gtttaccttc gatttagttt ttttctgaag aagccgtcac tccggcttaa
     1501 ggtatttcga aatatcctcg gtcaagaagg ttccctcatg catcggcctt acctcaacca
     1561 ctccgtgcag cgcagtcgga gaacgcgccg cccacgagat tgccgcatcc aggtccggta
     1621 cttcgatgat gtgaaacccg cccaactgct ctttggcatc cgcgtacggt ccgtccagca
     1681 cctgggtctt ctcattgcgg attcgcactg tcgtcgccgt gggcgtcccc tgcagcccgc
     1741 cgctggactt cagaacgccg gccttcgtca tcgcttccat gtacgcgcga tataccccca
     1801 accagtgagc ctgctcttcg cgcgtcagct gcggccatcc cgattcgtgc acgtaatcca
     1861 gcaacatgta tttcattggt atctccccca cgcgaatttc caaagatctc gtgcaatcgc
     1921 gatcgttctc caagtcgccg ctcatcgcca gatctgccgg acctcgactg ctccgtggcc
     1981 ggccccgggg caacgttggg cccaggacaa agccgcctgc cgatccgtta catcgacgat
     2041 gtagaaaccg cccaactgct ccttggcatc cgcgtacggc ccatccagca cgctcggctt
     2101 cccgtcggcc gctcgaacgg tcgcagccga agcactcggg tacagccgac caccgctctt
     2161 gaacacaccg gccttttgca tggcttcggt atacgccgtg taagcggcca tgccttgctc
     2221 ctgctgcgct tggctcaatt ttggccagcc agcctcattc acgtaaatca tcaacatgta
     2281 atccatagca gacctccgtg gtcgtgacgg gcgggccctg tccagcccaa ttccctgctt
     2341 cccatacaat gacgctcggc ttagggccga tttcgacagg cctatggaac cttttttggg
     2401 gcgggattca ggcggtctgc ttcagggcac gctcgactag cgtcaggcag cgctcggcct
     2461 gctcgcgcag aagctcctcg cgctcggtcc ggtcgcgcaa cccactgtgc agctcgtcgg
     2521 caatggccag ggcggccagc accgcggcct tctgggtatc tattgtggtg gtcgcatcgg
     2581 caattgcgcg catcttttcg tcgacgtaaa ccgccagctt ctgcacgtac gcgggttcca
     2641 gatcgcccgc aatagcgtag gtttggccga aaatttgcac tctggtcgtt ggcatgaagt
     2701 cagcactata cggctaagcg caaaggtagc acaaaaagac catcgattcg cggcttagga
     2761 attcaaccgc cgccgaacac atcgcagttt ttgttgggtt agaagtgcaa caccacgccg
     2821 acgccgatct ggaaattgtt ctgcgaatcg gagtacagca gggtgtggac ataatctgct
     2881 tcaccgcgga acgacaaccg cggattgaag aaccagtcga cgccgccgcc cgcctgcaaa
     2941 gcaaacgcgt tcttgctgga gttggcgatt tgcggattga cgtgtgtcat gcccgccaga
     3001 acatgcgccc atggtgagag gcggtgcggc tgtcgattcc acaatatgcg cccgccgccc
     3061 gtgaacagca cgtatttgga catttcgcca ccctggaaga catcgccacc aaacgccgaa
     3121 accagactgc cttccagcgc gaaccattca ttgagggaat acgcaatgga ggtgtggatc
     3181 ccgctcaggt ttgcggagaa cggcttggac tcgaaatgca cgtattcata gccaaccgct
     3241 agatcccagc ggtagccgat atcgccgccg cggccaaatt gcggcgcacg cggcgcgtcc
     3301 ggcaacgccg ccgaaggaac tggcgaaagc agggccgctg gcgcggctgc agcgggaata
     3361 gccagagaag gcacaagtgc gccggtggaa tccgcggcgg cggcagctgg cgcgacgaat
     3421 gaaggttgcg gggtgcgatc gaaactcgag aacgaggcct gctgcgccgg caatgaagca
     3481 gagacagcaa gggcgacaaa cacaaccaag acgaggcgtt tcatttgcga tccttaaagg
     3541 gcagacccca cgacagggtt ggctggcact tataacgtaa acaggagaag gggtgtttgt
     3601 ctatggcgat tcacagatga gcagcagaaa tcaaacagcg ccggctaagg tacgacgtaa
     3661 gccgtgctgc cggagttcgt aaagtcaaag cgcaccagcg cggatgtgtt cccggacgga
     3721 gcgaatcgcc aatcgcgcgc cgccttcaat gccaggttcg cgaaaaactg gctgcccgcc
     3781 ccggagtcga gcgccgccgc cgacaccgag ccatccggat tcaactgcac tcgcacgttg
     3841 atgcgcaccg tgccgcgaat cgtgctgcgc gccttttgtg aaacgtcggg aaccacccgg
     3901 tgaacaactt gtgcagctcc caaaaccgcg ggcgtagtgg aacgcgagga agatacgctc
     3961 ggcgcaaccg gtgcctggtt ctccgccgga gatacagccg tggacggcgg cgcgacggcc
     4021 ggtggtttcg acgccggcgc ggtctttgac gcggatggcg tggtggactc ttgcgtggac
     4081 ggtgcactct tcgctgccga agcaggcgta ctctcggctg ctggcttcgt tgctggggac
     4141 gacgcggctg cagtcttcga ggtaaccgaa gaatcggcgc tgttccttag catccgcgga
     4201 acaatcagca ccgccaggac cagcgtcccg gccgctccaa ccaaaaaata ctttagcgaa
     4261 ccactcgacg cgcgtacctc gggcactcgc cgtggcgctg cgatggtcac attcacgtta
     4321 ggcagcgatg agaccgggac gtgcgatgga gccttggccc gcgtctcggt tggcgaaacc
     4381 ggtgacagcg gcaccgcgag gggatcaacg gtctgcaaag gcatcggtgc tgctggcgtc
     4441 gtgcgcgcgg tcgcggttcc ggcagaagct tgtgcagcgg ccgccagtgg ctgcggcact
     4501 ggcacggttt caggttcctg cgccgcgctc gccgaagcag caacggtcgg aggctcagcc
     4561 ggtgacttga ctgacgcctc cactggcaca ggagcccgaa caggtttagg tttcgccaca
     4621 aacgacggat cgagcgcgga ccgaatctca tcgagcgaaa tccgttccgt cgcatcctgc
     4681 accagcgctc cgcgaatgac ttccgcgaaa ggactcggca actgcggtaa cacgtagtgt
     4741 ccgctcgaat catggttcgg caaatactgc gtcagcgtct cgtagagcga aattcccagc
     4801 gaccatacgt ccgacgccgg aaacagcagc gactccggag gagcataagg cgcagccatc
     4861 ggccacgatg tggaagattc acccgcagga agaatccggt ccggtgacaa tttgatggta
     4921 tctcgaatgg ccagaatgtt ggacggcttc acgccgccat gcaccagccc tttttcgtgc
     4981 aggaaatcca gcgtggccag caccggcgcc atcatgcccg aagcttcctc cggcgaaagc
     5041 gcgcgctgcc ggagcagttc gcccaaattt tcgctggcca gatccgtcac cacaaataaa
     5101 cacggagaac catccatcgc gcatcgccca aaatgcaaaa tacgcaacag tccgggatgt
     5161 gacagcgccg ccacactctt ccaccgagcg agttgctgct cagcagtttc cggatcctcg
     5221 ggaatcagct tgatggcggc ctcaggcgcc ggttcgctcg gacgctgatt gggcaggtcg
     5281 gtcagaaaga ccgccgaatt tggcgaaacg cccagcaggc ggcgtagcgg aaaccgcccg
     5341 tccaccaact ggccgagaag atgttgtgcc gtttctggca tggagaaccg ccattcccgc
     5401 ccgcggggta gcgggcggag gagttagact tagcgaaaca gactcataaa tagcagcgca
     5461 agcgcgtttt caggattcca ggcgaaccct gacgtgccaa acgcgatggg ccggggaggc
     5521 ttacaggcat tgtgcaggcg cgcgctagga aaggaaaggg catgccgcca aaaaggcatc
     5581 ctcggtaaaa aaaaaggcgg acaagactcc acgcccttcc cgaaagttcc catcgagcca
     5641 ggtgccctac gaacccatca agaacagcgg cttgttcgaa aagctgttgt atttgggccg
     5701 caaccgttcg gtgtcgtatt gatcgttcac gtccacaaat ttcttggtgg cggtcacggt
     5761 agtgatgggt aagtggctgt agcgcacttc acgaaacacc accaaatggc caaacgtttt
     5821 gttcaaaatc agatccagcg ccaggttgcc gtatgccatc ggcacgatcg aatcaatcgc
     5881 gtccggttcg ccggagcgca ccaagtaccc gagtttctgg ctgatgactt cgatcttctt
     5941 gccgcccttg ttgaatttcg cggaacggtt cttcacctct tccgccacca ggtcgccaat
     6001 gccgcccaac tttttgtgcc cgtaagcgtc ggcggactcc ccttcgaaaa tcatctcgcc
     6061 gccttcgaac atggctcctt cggataccag cgccaccgaa tagcgacttg ggttcttgat
     6121 ccggtcggaa accagcaact ccgtgagccg atcaatgttg aacttgtgct cgggaatcac
     6181 gcagcggtcc gccgctccgg ctagtgtcgg cagcatcgcc gtgtagccgg cgtagcgccc
     6241 aaacacttcc aggatcaaga accgctcgtg cgaccccgcc gaagtgcgaa tgctgttggc
     6301 aagctgaatc gtgcgcgaaa cgcaggtgct gaagccgatg cagtaatccg tgccgggtac
     6361 gtcgttgtcc atcgtcttcg gaatcgcaac gacgtggaaa ccttccttgt gcagacgcac
     6421 tccgtagctc agcgtgtcgt cgccgccgat gggcaccagg taatcgatgc ccagccattc
     6481 cagattcttt aagacttccg ccgtaaggtc gttcttttct tcggtgaact ggctgcgcaa
     6541 gtgttccggc acactgccct tcggcgtttt tccaggattc gtgcgcgagg aatggagaaa
     6601 agttccgccg gtgcgactgg cgcggttcac cacgtcttcg gtcaaaagtt ggtagttgtt
     6661 cgaattgtcg taatctttgt cgcggacaat atcaatcagg ccgccccagc cgtgccggat
     6721 accaatcacc tgataccctt cgcgcagggc gcgataggtg acgccgcgaa tcgcggggtt
     6781 cagtccaggg acgtcgccgc cgcccgtcaa aattccgatg atgccctttt tcgtactgac
     6841 attagccatg ttttgcgcga ccttctcggt aaagttgaac cgataatttt acacggagtc
     6901 gcg
//
LOCUS       DASTPM010000465         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_477677, whole genome shotgun sequence.
ACCESSION   DASTPM010000465 DASTPM010000000
VERSION     DASTPM010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477677"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFR42_08245"
     CDS             complement(<1..514)
                     /locus_tag="VFR42_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0369183.1"
                     /translation="MRKCVLLLCFLALACDRNCGQVSSTGATATLTVIKAGVLIDGET
                     ETPRKNQLIFVRGERIENVSDASAAIPAGAKVIDLSSATVLPGLIDAHTHIFLWGEKP
                     DKGGYDANILKAGIALRAARATFACRRALEQGFTTLRDVETEGAGYGDVEIKQAIEEG
                     TIPGPRLFVAT"
     gene            complement(637..1434)
                     /locus_tag="VFR42_08250"
     CDS             complement(637..1434)
                     /locus_tag="VFR42_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEU0369184.1"
                     /translation="MTATYDARIERAQHLKKIYPFAAEILTFYEALCAVQAQVITGLP
                     GVRPPAKATLRERLHVDLVLPCAHEALRKLIPVSPEPLKAFIAGYLRGSNERLAVSLQ
                     TYVDHGGTDEAPADAREELLARILIEPYAELVAPQITVVSAGVTGNSCPRCDGRPLLG
                     VLRPEGDSGKRFLMCAFCSSEWEFRRVYCAYCGETREQSLPVFVAEKFPHIRVEACDT
                     CRHCLRTVDLTKDGNAVPAVDDLAAIPLALWAEEYGYQRIHGNLLGT"
     gene            complement(1431..2093)
                     /locus_tag="VFR42_08255"
     CDS             complement(1431..2093)
                     /locus_tag="VFR42_08255"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEU0369185.1"
                     /translation="MSDRAEQILPHGRVLRYPFAERANHWIAAFSYIYLLLTGLAFWS
                     PWLFWIAVGLGGGQISRTLHPWVGLFFFYAVMRMYGYWAPQMRNTEVDKQWWNSLRHY
                     IQNEDDKMPPAGRYNAGQKMLFWSFFFAAIVLLLTGAVLWFPEWIPWNLRWLRYLSVF
                     LHPVAALFTIANFLIHIYMSVFAERGAFGSVIRGDVSLDFAKRYHPGWYEEIVGKPAP
                     RK"
     gene            complement(2090..>3033)
                     /gene="fdxH"
                     /locus_tag="VFR42_08260"
     CDS             complement(2090..>3033)
                     /gene="fdxH"
                     /locus_tag="VFR42_08260"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEU0369186.1"
                     /translation="GIQGIPGEDRESLDENEPRGATHSKISAHAGSVPGAGTQREYTV
                     CKLIDTTTCIGCKACEVACMEWNDLPFGETVFDNTYQTMPETRWNYWNLILFNEHEKE
                     DGSLMWLMRKNQCMHCAEPGCLAACPSDGAIVQYTNGIVDFQQDKCIGCGYCITGCPF
                     NIPKFNAETKRVYKCTLCTDRVSQGLEPACIKSCPTGCLHFGSKDDMKYLAEQRAKQL
                     RDHYDFPSAGVYDPPGVGGTGVIYVLHDIKQPEIYGGLPKDPHVPMSVRLWKGPLKWL
                     GNIAMVGGVIGLFVHYLRFGPKEHNVELKEPHEGAEP"
BASE COUNT          674 a          943 c          765 g          651 t
ORIGIN      
        1 gcgttgcgac aaatagccga ggccccggaa tggtgccttc ctcaatggcc tgcttgattt
       61 ccacgtcgcc gtagcccgcg ccctccgtct ccacgtcgcg cagagttgta aagccttgct
      121 ccagggctcg ccggcatgcg aaggtcgcgc gggccgcgcg caaagcgatg cccgccttaa
      181 ggatgttggc gtcatagccg cctttatccg gcttttcgcc ccataagaaa atatgcgtgt
      241 gagcgtcgat caagcccggc aataccgtgg cactcgaaag atcaatcacc ttggcgcccg
      301 ctggaatcgc ggcggatgcg tccgaaacgt tttcaatccg ctctcctcgc acaaaaatca
      361 gctgattctt gcgcggtgtt tccgtctcac catcgatcag tactccagcc ttgatcactg
      421 tgagcgtcgc tgtggcgcca gttgaagaaa cctggccgca gttgcgatcg caagccaacg
      481 ccagaaaaca cagaagtagc acgcacttgc gcattctgac ctccgggaaa gcggtactat
      541 gctgcaactg ccggttccaa cgcaactttc cttttaattt ccttttaatt gcgaattcaa
      601 agaaatccga attgcatcgc gaaagccccg ggttggtcag gtccccaaca gatttccgtg
      661 gatccgttgg tagccgtatt cttccgccca aagcgccagt ggaatcgcgg ctaggtcatc
      721 caccgccgga acggcgttac cgtctttggt cagatcaaca gtgcgcaggc agtgccggca
      781 cgtgtcgcag gcctctacgc gaatatgcgg aaacttctcc gccacaaaca ccggcaatga
      841 ctgctcgcgg gtctcgccac aatacgcgca ataaactctg cgaaactccc attccgacga
      901 gcagaacgca cacatcagaa agcgctttcc gctgtcgcct tctggccgca gaacgcccag
      961 gagcggcctt ccgtcgcagc gtgggcacga atttccggtc accccggcgg atacgacggt
     1021 gatctgcggc gccaccagtt ccgcataggg ctcaatcaag attcgcgcca ggagttcctc
     1081 tcgcgcgtcc gccggtgcct cgtccgttcc gccatgatct acataagtct gcagcgaaac
     1141 cgccagccgc tcgtttgagc ctcgcaaata cccggcaata aatgctttca acggttcggg
     1201 cgaaactgga atgagtttcc gcaaagcttc gtgcgcgcat ggaagcacaa gatcaacgtg
     1261 caaacgctcc cgcaacgttg ctttagccgg cggtcggacg ccgggaagtc ctgtaattac
     1321 ctgcgcctgc acggcgcaaa gcgcttcgta aaaggtcagg atttctgcag cgaatggata
     1381 aattttcttt agatgctgcg cgcgctcgat ccgcgcatcg taggtcgcgg tcatttacgc
     1441 ggagcaggtt tgcccacaat ctcctcgtac cagcccgggt ggtaacgttt ggcaaaatcc
     1501 agcgaaacat cgccacgaat caccgaaccg aacgccccgc gctcggcaaa aacactcata
     1561 tagatgtgaa tcaaaaagtt ggcgatcgta aacaatgccg ctacgggatg cagaaacacc
     1621 gagagatacc gcaaccagcg caaattccag ggaatccatt ccgggaacca cagaaccgcg
     1681 ccggttagga gcagcacgat ggccgcaaag aagaaactcc agaacagcat tttctgtccg
     1741 gcgttatagc gaccggctgg cggcattttg tcatcttcgt tctggatgta gtgccgaagc
     1801 gagttccacc attgcttgtc cacttccgtg tttcgcatct gcggcgccca atagccgtac
     1861 atccgcatca ccgcgtaaaa gaaaaacaat cctacccacg gatgcaatgt ccgcgaaatt
     1921 tgcccgccgc cgagccccac cgcaatccaa aacagccaag gagaccagaa cgcgagcccc
     1981 gtcagcagca aatagatgta tgaaaacgcc gcaatccagt gattcgcgcg ctccgcgaac
     2041 gggtatcgca acactcttcc atgaggaaga atctgctccg cccgatcgct catggctccg
     2101 ctccctcatg tggttccttc aattccacat tgtgctcttt gggtccgaag cgcaggtaat
     2161 gcacaaacaa tccgatcacg ccgcccacca tcgcgatgtt ccccagccac ttgagcggtc
     2221 ccttccacag ccgcacactc attggaacgt gtggatcctt cggcagcccg ccataaattt
     2281 ccggctgctt gatgtcgtgc aaaacgtaaa tcacccctgt tccgcccacg cctggcggat
     2341 cgtagacccc cgcactcgga aaatcatagt gatcgcgcaa ctgcttcgcg cgttgttccg
     2401 ccaggtactt catgtcgtcc ttgctgccga agtgcaggca gcccgtcggg cacgatttga
     2461 tgcacgctgg ttcaagtccc tggctgacgc gatccgtgca caacgtgcac ttgtacacgc
     2521 gcttcgtctc tgcgttgaat ttcggaatat tgaatgggca acccgtgatg caatacccgc
     2581 aaccgatgca tttatcctgc tgaaaatcga cgatgccatt cgtgtattgc acaattgccc
     2641 cgtcagaagg gcacgctgcc aggcatcccg gctccgcgca atgcatgcac tgattcttgc
     2701 gcatcagcca catcagcgag ccatcttctt tctcgtgctc gttgaacagg atcaaattcc
     2761 aataattcca ccgcgtttcc ggcatggtct gataggtgtt gtcgaaaacg gtttccccga
     2821 acggcaaatc gttccactcc atgcaggcta cttcacaggc tttgcagcca atgcaagtcg
     2881 tggtgtcaat gagtttgcag acggtgtatt cgcgttgcgt gcccgctccg ggcacgcttc
     2941 cggcatgtgc agagattttt gaatgcgtag cgcctcttgg ctcattttcg tctaggcttt
     3001 ctcgatcttc accaggaatc ccttgaattc cgg
//
LOCUS       DASTPM010000466         5810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_304515, whole genome shotgun sequence.
ACCESSION   DASTPM010000466 DASTPM010000000
VERSION     DASTPM010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304515"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..762)
                     /locus_tag="VFR42_08265"
     CDS             complement(49..762)
                     /locus_tag="VFR42_08265"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU0369187.1"
                     /translation="MLDLKGNRITYFGHSAFALTTSSGQVALIDPWIMTNPRCPESLK
                     KLSRVDAIFLTHGHTDHMGDLLAIAKQFRPKLVAIFETCLWFAEKGFGKEVLPMSKGG
                     SQQVGDFRVTMTHAMHSNSIEDNGQRIYGGEPAGYVVELPGGVRIYHAGDTALFGDMK
                     LIAELYQPQLAILPIGDLFTMGPRETAHAIRFLNVKQVIPMHYATFPVLTGTPEQLRE
                     EAKDVAGLTIHALKPGESL"
     gene            1019..1282
                     /locus_tag="VFR42_08270"
     CDS             1019..1282
                     /locus_tag="VFR42_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEU0369188.1"
                     /translation="MAYVIAEPCIGTKDTACVDVCPVDCIHPRKDEAEHEGVTQLYIN
                     PTECIDCGACVPVCPVTAIFAMEDLPEKWQAFAATNTDWYAKK"
     gene            1443..3218
                     /locus_tag="VFR42_08275"
     CDS             1443..3218
                     /locus_tag="VFR42_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0369189.1"
                     /translation="MKNIVLIFVCVATPFGASRHAAAQQPPASVATKTPVARPVTLEG
                     DWAGTLQVGETELQLVLHLSRDAQGQWHAKLDSLNQSVFGMEASSVTHEEDALHFEIA
                     SVGAKFQGKIQPDHRTIRGVWEQSGSGLPLKFEKRSASAGSRAAAAAVSKAEGTWQGA
                     IEVTNMRMRLQMHVSHDDKGKLLASVDSLDQGIQGIPASKVTEANGELSFEIPTFQAE
                     YHGTLNASRNEISGEWTQNDSVEKLDFRRSDQPLEVRRPQNPAKPYPYSVAEISFSSG
                     DSKTTLAATLTIPAGTGPFPAALFIGGSGPTDRDETVAGHKPFLVLADLLTKKGLAVL
                     RYDKRGTGQSSGNYEQASISDLRSDAQAALNYLKSRKEVDPKRVGILAHSEGGILASQ
                     IAAKAGDLDWLVLLATPATTGERTLLRQSELVARTGGLPEEQIARSQQFDRMAYAAVR
                     EAKSATALEAKLNDLVEKSGLSASMPAAALQAQIRLMTTPWFRQYLDFDPAPLMEQIK
                     CPVLALNGDRDLQVDANDTVPLLRQAYEKSGNKDFTVMEIEGVNHLFQKAQSGSPALY
                     GAIEETIAPEVLNAVGSWLAKHTPQ"
     gene            3324..5450
                     /locus_tag="VFR42_08280"
     CDS             3324..5450
                     /locus_tag="VFR42_08280"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEU0369190.1"
                     /translation="MPPKFHLSLLIHAHQPVGNFEDVLERCYRQGYLPFAQLLEQHPA
                     IRVGLHYSGPLLLWMEAHHPEYFDLLRTLVHRKQVEMVGGGFYEPILAVIPAEDQREQ
                     IERLSAYIAEHFGERPSGAWLAERVWEPQLPFVLASAQVGYTLVDDFHFLCAGFEPAE
                     LLGAYQAEDRGKSVRLFAGQKALRYLVPWEKVSDAISYLKETAVMHPDGIAIFGDDME
                     KFGVWPGTFEHCYTDHWLEDFFKALEANADWLSLTPPGEYAQTHAPLGRADLPTASYP
                     EMMEWALPTSVRQRFHLVQQEFTKRPEVAAFLRGGSWRSFFRKYSESNLLHKKMLRAS
                     TRLAAVPQRRTESRSSQEIAHARDLLLRSQCNDAYWHGVFGGLYAPHLRTELWRNLIR
                     AEAAADRLTPGGQIGRVELLDYDADGKNELLFTGPEYQALLKPSDGGTLAALDFRPVC
                     ATLVNSLQRRPEAYHARLREVSLAKTEAGTPLHEQSRVKEPNLERFLQYDRWPRHAFR
                     ILVFDPKRTHAEYESLQLMEDPSFAAGEFAVQSSGPFHADLTRETTIRSNHPSSIATP
                     VFVTKRFSFSPAPGGCEVSCEVGLKLKEPLDRAVIVAVETIVNLLAPTEADRFFETSS
                     GPQSLRYSGALPGPVLHMQDGWQKIRVTLHSPGSENFWIAPIETVSESEDGFERVYQG
                     SQILARWRLSTQKILSARVVWRLETI"
     gene            complement(5484..>5810)
                     /locus_tag="VFR42_08285"
     CDS             complement(5484..>5810)
                     /locus_tag="VFR42_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369191.1"
                     /translation="AFLCRIFLNGWSATPRGISIDEQTALLIDDSGNGSVVGVSTVYF
                     MQAPGAPQVCQSKTPLTYQNISVYRIVAGGTFNLSTWTGNNGVNYTVSANAGVLSSTQ
                     SGGSIY"
BASE COUNT         1231 a         1697 c         1661 g         1221 t
ORIGIN      
        1 gaacctgggc tttttcgtcc gcaacttttt tcgctagaca acatttctct aaagggactc
       61 gccgggcttc agggcgtgaa tcgtcaggcc cgcgacgtct ttcgcttctt cgcgcaattg
      121 ttccggcgtg cccgtcagca cggggaatgt cgcgtagtgc atcgggataa cctgcttcac
      181 attgagaaag cggatggcgt gagctgtttc acgcgggccc atagtgaaga gatcgccgat
      241 gggaagaatc gcgagctgcg gctggtagag ctcggcgatg agcttcatgt cgccgaacag
      301 agccgtgtcg cccgcgtgat aaatgcggac tccaccggga agttccacaa cgtagccggc
      361 gggttcgccg ccgtaaatgc gctggccgtt gtcctctatg gagttcgaat gcatggcatg
      421 cgtcatggtt acgcggaaat cgccaacctg ctgcgagccg cctttgctca tgggcaaaac
      481 ttctttgccg aagccctttt cggcaaacca cagacacgtt tcgaaaatcg ccaccagctt
      541 aggacgaaac tgtttggcga tggcgaggag atcgcccatg tggtcggtgt gcccgtgcgt
      601 caggaagatt gcgtccacgc gggaaagctt cttcagtgat tcggggcagc gcggattggt
      661 catgatccac ggatcaatca gcgccacttg cccggaagaa gtcgtcagag caaaagcgct
      721 gtggccaaag tacgtgattc ggtttccctt gagatcgagc atgagcaagc aagcctcggt
      781 tatgaattgg gctgtgattc aaggctaaac ggaaagctgc ttgacgaaca agccgtgatg
      841 agccgttacc gcggagagtt gagaaactcc atcgcgaccg ggtacactaa gtacgtcgac
      901 gattacggga agcccccgaa tcacccgtac aacccagggt ttgtagaaac cttgaactga
      961 aatttgtgca ggagcgccga tgacgcggcg ctggcgtgcg aaacaaagga gaagctgaat
     1021 ggcttacgtg attgccgaac cgtgcatcgg caccaaggac accgcctgtg tggatgtgtg
     1081 tcccgtggat tgcatccacc cccgcaaaga cgaagcggaa catgaaggcg tgacacagct
     1141 ttatatcaat ccgaccgagt gcattgactg tggcgcctgc gttccggttt gcccggtcac
     1201 cgcgattttc gccatggaag acttgccgga aaagtggcag gcgtttgcgg ctaccaacac
     1261 cgattggtac gcgaaaaagt aagcagagac gatcgtagga ttctgccgtg cggcacgctt
     1321 tggcgtgccg ttttgttttt gctccggggc agcggtgcgg cgatgcgctg ccaaccttac
     1381 aggcagcgcg ctcatctatg catgggccag gcaagtgtgg ggcaggttca aggatcattc
     1441 cggtgaagaa tattgttttg attttcgtct gcgtcgccac gccgtttggt gcaagcagac
     1501 acgcagcggc gcagcagcct cctgcgtcgg ttgcgaccaa aacgcccgtc gctcgccctg
     1561 ttacgctgga aggcgattgg gcaggcacgt tgcaagtcgg cgaaacagaa ctgcagcttg
     1621 tcttgcatct gtcccgggat gcgcaaggcc agtggcatgc caagctcgac agcctgaatc
     1681 aatcggtatt cggaatggaa gcgtcgagcg ttacccacga agaagatgct ctacactttg
     1741 agattgcttc cgtcggcgca aaattccagg gaaaaattca gcctgatcat cgaaccatcc
     1801 gcggcgtgtg ggaacagagc gggagcggac tcccgctcaa atttgaaaaa cgctcagcca
     1861 gtgcaggctc tcgcgccgcc gcggccgcgg tatccaaggc ggaaggcacc tggcaaggcg
     1921 ccatcgaagt gaccaatatg cggatgcgcc tgcaaatgca cgtatctcac gacgacaaag
     1981 gaaagttgct tgcatccgtg gacagcctcg accagggcat ccagggaatt cccgcttcga
     2041 aagttacgga agcaaatggc gagttgagtt ttgagattcc cacatttcaa gccgaatatc
     2101 atgggacctt gaacgcctcg cggaatgaaa tatccggcga gtggacccag aacgacagcg
     2161 tcgaaaaact cgacttccgc cgctccgatc agccgctcga agtgcgccgt ccgcaaaatc
     2221 cggccaagcc gtatccatac tccgttgcgg aaatcagttt ttcgtccggc gatagtaaga
     2281 ccacgcttgc tgccacgttg acaatcccgg cagggacggg accttttccc gcggccctct
     2341 ttatcggcgg ttccgggccc acagaccgcg atgaaaccgt tgcgggccat aaaccctttc
     2401 tggtcctggc cgatctcctc accaaaaaag ggctcgccgt tctgcgctac gacaaacgag
     2461 gaaccgggca gtccagcgga aactatgaac aagcttccat aagtgacctt aggagcgacg
     2521 cacaggcggc cctgaattac ctgaaaagcc gcaaggaagt ggacccgaag cgcgtcggca
     2581 tcctcgctca tagcgaaggt ggtatcctcg cctcccaaat cgcggcgaaa gcaggcgact
     2641 tggactggct ggtattgctg gcaacgcccg ccaccaccgg cgagcgcacg ttgcttcgcc
     2701 aatcggaatt ggtcgctcgc accggcggct tgccggaaga gcagatcgct cgcagccagc
     2761 aatttgatcg catggcttac gccgcagtgc gcgaagcgaa gagcgccact gcactcgaag
     2821 cgaagttaaa cgatctcgtc gaaaagagcg gactcagcgc ctccatgccc gcggccgcac
     2881 tgcaagcgca aattcgtttg atgaccacgc cgtggttccg gcagtatctc gactttgacc
     2941 ccgcgccgct gatggagcag atcaaatgcc cggttctggc cttgaatggc gaccgcgacc
     3001 tgcaagtgga cgccaacgac actgtgccgt tgcttcgcca ggcttacgaa aaaagcggga
     3061 acaaggactt tacggtgatg gaaatcgagg gcgtcaacca ccttttccag aaagcgcagt
     3121 ccggttcacc ggcgctgtat ggcgcgatcg aagagaccat tgcgccggaa gtcctcaacg
     3181 ctgtcggctc ctggctggcc aaacacacgc cgcaatagcg cgaatcgctc cgcaatagtt
     3241 gcgaatttgc ggcatgctcc tccgccgcgc gattgcaaac gaaatgtgcg cggtatcata
     3301 ggaggggagc ttgaaccgtt cctgtgccgc caaaatttca cctctccctg ttgattcacg
     3361 cgcatcagcc ggtcggcaac tttgaagacg ttctggagcg ttgctaccgg cagggctatc
     3421 tgcccttcgc gcagttgctc gagcagcatc cggcgatccg cgtggggttg cactactccg
     3481 gcccgctcct gctttggatg gaagcgcacc atcccgaata ctttgacctg ctgcgtactc
     3541 tggttcatcg gaagcaagtg gaaatggtcg gcggcggatt ctatgagcct atccttgcgg
     3601 taattcccgc cgaagatcag cgcgaacaga tcgagcgcct ctctgcctac atagcagaac
     3661 actttggcga acggccttcg ggtgcgtggc ttgcggaacg cgtttgggag ccgcaactgc
     3721 catttgtgct ggcgtccgcg caagtcggct atacgctggt ggatgatttt cactttcttt
     3781 gtgccggatt cgagcccgcc gaactcttgg gggcttacca agctgaagat cgcggcaaaa
     3841 gtgtccgcct gtttgccggc caaaaagcgc tccgctatct ggttccctgg gagaaagtct
     3901 ctgacgccat cagctattta aaagagactg ccgtcatgca tcccgacggc atcgccatct
     3961 tcggtgacga catggaaaaa ttcggtgtgt ggcccgggac gttcgaacat tgctacaccg
     4021 accactggct ggaggatttt ttcaaagcgc tcgaagccaa cgcggactgg ctttccctga
     4081 ctccgccggg cgaatacgca cagacgcatg ctccgcttgg gcgcgccgat ctgcccaccg
     4141 cctcttatcc ggaaatgatg gaatgggctt tgcccacttc ggtgcgccag cgttttcatc
     4201 tggtgcagca ggagttcacc aagcgccccg aagttgcggc gtttctgcgc ggcggttcct
     4261 ggcgcagctt ttttcgcaaa tattccgaat ccaatttgct gcacaagaaa atgctgcgcg
     4321 ccagcacgcg cctcgcggct gttccccaac gccgcaccga atcgcgcagc tctcaggaaa
     4381 ttgcccacgc gcgcgacttg ctcctccgct cgcaatgcaa tgacgcgtac tggcacggcg
     4441 tgtttggtgg cctctatgcc ccgcacctgc gcaccgagct ttggcgtaat ctaatccgtg
     4501 ctgaagccgc cgccgaccgc cttacgcccg gcggccagat cggccgcgtc gaactgctcg
     4561 actacgacgc cgacggcaaa aatgagcttc ttttcaccgg ccccgaatat caggcgctcc
     4621 tgaagccctc ggatggtggc acgctcgctg cgctggattt tcgccctgtc tgcgcaactt
     4681 tagtgaattc cttgcagcgt cgccccgagg cttaccacgc gcgacttcgc gaagtttccc
     4741 tagcgaaaac ggaagccggc actcccctgc acgaacagtc ccgcgtaaag gaacccaatc
     4801 tcgaacgctt tctgcaatac gatcgctggc cgcgccacgc ctttcgcatt ctggtgttcg
     4861 atcccaaacg cacgcacgcg gagtacgaat cgctgcaact gatggaagat ccgagttttg
     4921 cagcgggtga atttgcggtg caatcctccg ggccatttca cgcggacctg acccgtgaaa
     4981 ccacgattcg ctcgaatcac ccgtcgagca tcgctacacc ggttttcgtc accaaacgct
     5041 tttctttttc gcctgctccc ggcgggtgcg aagtttcttg cgaagttggc ttgaagttga
     5101 aagagccgct cgaccgcgcg gtgattgttg cggtggagac tatcgtcaat cttttggcgc
     5161 caaccgaggc ggaccgcttt tttgagacgt catcgggccc gcaaagtttg cgctacagtg
     5221 gcgcactgcc gggcccggtg cttcacatgc aggacggttg gcagaaaatc cgtgtcactc
     5281 tccattcgcc cggttcggag aatttctgga ttgcgcccat tgaaaccgtt tccgaatcgg
     5341 aagacggctt tgagcgggtc tatcagggat cgcagatttt ggcgcgctgg cgactcagca
     5401 ctcagaagat cctctctgcg cgcgtggtct ggcgcctgga aaccatctga acttcttgac
     5461 gctggatttt tttgacgaat ggtttagtag atcgatccgc cggactgagt cgaactcagc
     5521 acgcctgcgt tcgcggacac ggtgtagttc actccattgt ttcctgtcca ggttgacaga
     5581 ttgaatgtcc cgcccgcaac gattctatac acgctgatgt tctggtaggt gagcggtgtt
     5641 ttggattgac aaacttgtgg cgcaccaggc gcctgcatga aatagacggt gctaacacct
     5701 actaccgagc cgttgccgct gtcatcaatc agcaacgcgg tttgctcatc gattgaaatg
     5761 ccccgcggcg tggcggacca tccattcaga aaaatccggc acaaaaatgc
//
LOCUS       DASTPM010000467         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_391455, whole genome shotgun sequence.
ACCESSION   DASTPM010000467 DASTPM010000000
VERSION     DASTPM010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391455"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1482
                     /gene="eno"
                     /locus_tag="VFR42_08290"
     CDS             169..1482
                     /gene="eno"
                     /locus_tag="VFR42_08290"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU0369192.1"
                     /translation="MSTKIAKVHARQVIDSRGNPTVEADVILEGGASGRAAVPSGAST
                     GEHEALELRDADKGKYLGKGVLKAVANVNGEIAKAVAGFDATDQRGLDHKMIMLDNTP
                     TKSRLGANAILAVSMASARAAAAATKQPLYKYLSKYSSDRSANLLPVPMMNILNGGAH
                     ADNSVDFQEFMVMPIGAPNFSEALRWGVEVFHALKAALKKHGYSTAVGDEGGFAPNCK
                     SNEEAIQIVLEAIGTAGYKPGEQVSIALDPASSEFYDKASSKYVFKKSDKSAHSSDAM
                     AAYWTGWVEKYPIVSIEDGMAEDDWAGWKSLTQSVGTKSSKNKIQLVGDDLFVTNTER
                     LSRGINDGIANAILIKLNQIGSVTETIDAIELARKAGYNSIISHRSGETEDTFIADLA
                     VATAAGQIKTGSASRTDRIAKYNQLLRIEEELGSAARFPGKAIFA"
     gene            1492..1998
                     /locus_tag="VFR42_08295"
     CDS             1492..1998
                     /locus_tag="VFR42_08295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0369193.1"
                     /translation="MSGPAAITGFRERFGEPADTPALVTLINVAFLPEQVAIEGDRIN
                     AEKLQPFFANGKFLVLEDTHGVAGCVYAEVRGQRGYIGLLALRPQLKGRGLGRLLMNR
                     AEAYLAAAGCEAADLRTISARDDLIPMYKHLGYEQSGTTPMPPEIPLKISCHFITMSK
                     RLQPQQRE"
     gene            2015..>2576
                     /locus_tag="VFR42_08300"
     CDS             2015..>2576
                     /locus_tag="VFR42_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU0369194.1"
                     /translation="MLKLADIFLAATIAAAPCIAQDNKPTTVASKTPLTAQDVVDRIK
                     AHVGIPWLEQTRDTFKAGDPRTPVTGIAVTMMATYAVLQRAAANRQNLIITHEPTFFD
                     DPDKAEPLPQGEQDPVLAEKRAFIEKHHLVIWRFHDHWHRMHPDGIELGNAHALGWEK
                     YQDPNNQYLFSLPETTVGKLADEVKAK"
BASE COUNT          609 a          809 c          678 g          480 t
ORIGIN      
        1 catacgtccg ccaaactcat acgtaagtct ttaccttgcc acatccaaaa aggtgcctcg
       61 taagttcttt gtttgccaca cggccgccag aaccacaggc ggtacctgcc tccgcccgct
      121 ggtttgctag aatgaacggc tcaccttcat ataggagact aggcagaaat gtccacgaag
      181 atcgcaaaag tccacgcgcg ccaggttatt gattcgcgcg gcaatccgac cgttgaggct
      241 gatgtcattc tggagggcgg cgcgtcgggt cgcgcggccg ttcccagcgg tgcttccacc
      301 ggtgagcacg aagcgctcga attgcgtgac gctgataagg gtaagtatct cggcaagggc
      361 gtcctcaagg ctgtcgctaa cgtaaacggc gaaatcgcca aggccgtggc gggattcgac
      421 gccaccgacc agcgcggcct cgatcacaaa atgatcatgc tggacaatac gcccaccaaa
      481 tcccgcctgg gcgcaaacgc catcctcgcc gtttccatgg caagcgcgcg agctgccgct
      541 gccgctacca aacaaccgct ctacaaatat ctgtcgaagt attcgagcga tcgcagcgcc
      601 aaccttcttc ccgtccccat gatgaacatc ttgaatggcg gcgcgcacgc cgacaactcg
      661 gtcgacttcc aggaatttat ggtgatgccc atcggcgcgc cgaatttttc agaagcgttg
      721 cgttggggtg tggaagtgtt tcatgcactg aaggccgcgc taaaaaagca cggctactca
      781 accgccgtcg gcgacgaagg cggcttcgcc cccaactgca aatcgaacga agaggcgatt
      841 cagattgttc tggaggcgat tggcaccgcc ggttataagc cgggcgaaca agtgagcatc
      901 gcactcgatc ccgcatccag cgagttctac gacaaggcca gcagcaaata cgttttcaaa
      961 aaatccgaca aaagcgcgca ctcgtcagac gcaatggccg cttactggac cggctgggtg
     1021 gaaaaatatc ccatcgtttc gatcgaagat ggcatggccg aagacgactg ggctggctgg
     1081 aaatcgctca cacaatcagt tggaaccaaa tcctccaaaa ataaaatcca gctggtcggc
     1141 gacgatctct tcgtgacgaa cacggagcgg ctctcgcgcg gcatcaacga cggcatcgcc
     1201 aacgccatcc tgatcaaact caaccagatc ggtagcgtca ccgaaacgat cgacgccatc
     1261 gaactcgccc gcaaagccgg ctacaactcc atcatttcgc accgctccgg cgaaaccgaa
     1321 gacaccttca tcgcggatct tgcggtcgcc actgccgcgg gccagatcaa gaccggttca
     1381 gcctcgcgca ccgatcgcat tgccaagtac aaccagcttc tccgcattga agaagaactc
     1441 ggcagcgctg cccgcttccc gggcaaggcc atcttcgcct gagcggcgcg catgtcaggc
     1501 ccggctgcga tcaccggttt tcgcgaacgc ttcggcgagc ctgcggatac tccggcactc
     1561 gtgacattaa ttaatgtcgc cttcctgccg gaacaagtag ccatcgaagg cgaccgcatc
     1621 aacgccgaaa agctgcagcc gtttttcgct aacggcaaat tcctggtgct tgaggatacg
     1681 cacggtgtcg ccggctgtgt ctatgccgaa gtgcgcggcc agcgcggcta catcggactc
     1741 ctggcgctgc gtccgcaatt aaaaggccgc ggtcttggcc gtctcctgat gaatcgcgca
     1801 gaggcctatc ttgccgctgc cggctgcgaa gcggccgacc tgcgtaccat cagtgcccgc
     1861 gacgatctca tccccatgta caagcatctc ggctacgaac agagcgggac aactccgatg
     1921 ccgcccgaaa ttccgctgaa aatctcctgc cacttcatta ccatgtccaa gcgtttgcag
     1981 ccgcaacaac gcgaatgagc catcgcgagg aaaaatgttg aaacttgccg atatcttctt
     2041 ggctgccacg atcgcggcgg ctccctgcat agcgcaggac aacaaaccaa caaccgtagc
     2101 cagcaagact ccgctcacgg cgcaggatgt tgtggaccgc atcaaagcgc atgtcggcat
     2161 tccgtggctt gagcaaactc gggacacttt caaagcgggc gacccgagaa ctccggttac
     2221 gggaattgcg gtgaccatga tggccaccta cgctgtcttg cagcgcgccg cggccaaccg
     2281 gcaaaacctg attatcacgc acgagcctac tttttttgac gaccccgata aagcggaacc
     2341 gcttccgcaa ggcgagcagg accccgtgct ggccgaaaaa cgcgccttta tcgaaaagca
     2401 tcatcttgtg atctggcgct tccacgacca ctggcaccgc atgcatcccg acggcatcga
     2461 actaggcaac gcgcatgcgc taggctggga aaaatatcag gatccaaaca atcagtatct
     2521 cttctctctt cccgaaacta ccgtcggcaa attggctgac gaagttaaag ccaagc
//
LOCUS       DASTPM010000468         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_968060, whole genome shotgun sequence.
ACCESSION   DASTPM010000468 DASTPM010000000
VERSION     DASTPM010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968060"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VFR42_08305"
     CDS             <1..843
                     /locus_tag="VFR42_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEU0369195.1"
                     /translation="DAIAVLASGGETVLAQIAPDSREVLGCNTRADLAEVDRIFRERK
                     REEMMAAGVTIQLPETVLIDPEVIAGADCSLEPGVQLLGKTKLGTRCKVGTGSVLLDA
                     ILGDDVIVRAHSLIEESRLDRGSIVGPFARLRTGTHLKAGARVGNFVETKKAVIGERA
                     KVPHLSYIGDAKLGSKTNIGAGTITCNYDGVNKHQTTIGNKVFIGSDSVLVAPVRIGD
                     GAYVAAGSAITENVPADALGIARGRQSNKKGWVAKKRREMAAGHAQAKPARRKPRQAK
                     KRRR"
     gene            929..1372
                     /locus_tag="VFR42_08310"
     CDS             929..1372
                     /locus_tag="VFR42_08310"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU0369196.1"
                     /translation="MGNWVNWLLVILGVLCVAAELALGALTGFDLALVGASLAAGGLV
                     GLIAQSWQTGLVSAAVFALLYFALFRRWLKAKLHVKEQASNVDAIVGKTGVVTKRIGP
                     AHDCGIVKVGSEEWRAELAQPDSAAREIGAHVQVVSVEGVTLRVQ"
     gene            1385..2293
                     /locus_tag="VFR42_08315"
     CDS             1385..2293
                     /locus_tag="VFR42_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU0369197.1"
                     /translation="MDNPFATFLPILLIALLLVGVLLLVRAARIVNQYEKGIIMRLGK
                     FHGLAPSGLTIIMPLTDSLIRVDMREQVISVPPQKLITKDNVTVEVDAVVYYKVVDPV
                     KSQFEVQDFGYACTTLAQTNLRNLIGDRTLDETLVARDMINTNLRHVLDEATNGWGVK
                     VTRVEVQKIDPPRDITEAMSRQMKAERDKRAAVLEADGVKQSQILQAEGFKQSEILKA
                     EGDAQARITRANAEAEAIRLVSTAAETFFKDRAEAMRRLEVLSSTLVHNTKYIVPSNA
                     GLVNVLGLDGAIGAAASAVAPPKPKG"
     gene            2445..>2628
                     /locus_tag="VFR42_08320"
     CDS             2445..>2628
                     /locus_tag="VFR42_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369198.1"
                     /translation="MNATPAKKKRLTDRTRMLLTLELAIVLPAASLMAFSVWNLHQIQ
                     RDKAIEAAIQRDFTSVL"
BASE COUNT          578 a          775 c          733 g          542 t
ORIGIN      
        1 gacgcgattg ccgtacttgc ctccggaggc gaaaccgtct tggcacaaat cgctccggat
       61 tcgcgcgaag tccttggttg caacacgcgt gccgacctgg ccgaagtcga tcggatcttc
      121 cgcgaacgca aacgcgaaga gatgatggcc gctggcgtaa ccattcagtt gccggaaacc
      181 gtgttgatcg accccgaagt catcgccgga gcagattgct cgctggagcc tggcgtacag
      241 ttgcttggta aaacaaaact cggtactcgg tgcaaagtcg gaacaggaag cgttctgctg
      301 gatgcaattc tcggcgatga tgtgatcgtg cgcgcgcact cgctaatcga ggaaagccgc
      361 ctcgacagag gctccatcgt gggcccattc gccagacttc gcaccggcac gcaccttaag
      421 gctggcgccc gtgtcggcaa ttttgtcgaa acgaagaaag ccgtgatcgg cgaacgcgca
      481 aaagttccgc atctttctta tattggtgac gccaaactcg gctccaagac caacatcggc
      541 gcgggaacca ttacctgcaa ctacgacggc gtgaacaaac accagaccac cattggaaac
      601 aaagttttta tcggcagtga ttccgtgctg gttgcgccgg tgcgcatcgg cgatggcgcc
      661 tacgtcgcgg caggttcggc gattactgaa aatgttcccg ctgacgcgtt gggaatcgcg
      721 cgcggccgcc agtccaataa gaaaggttgg gttgctaaaa agcgccgcga aatggccgcg
      781 ggtcatgcgc aagccaagcc cgcccgtcga aaacctcgcc aagcgaaaaa acgccgtcgt
      841 tagtctggca tttacacgcc ggtcattgaa acccgctgtt tctgatgtta tcttccgcag
      901 caacaattgt tcccgcaagg agctttccgt gggcaactgg gtgaactggc tgctggtcat
      961 tctgggtgtg ctttgtgtgg ccgccgaatt ggctctcggt gcgcttaccg gatttgacct
     1021 ggccttggtc ggtgcttcac tggcggccgg tggcctggtc ggtctgatcg cgcagtcatg
     1081 gcaaaccggc ttggtcagtg cggcagtttt cgctttgctc tatttcgccc tgttccggcg
     1141 ctggctgaaa gccaagctgc acgtgaaaga acaggcgtcc aacgtggacg ccatcgtggg
     1201 caagactgga gtagtcacca agcgcatagg cccggcacat gactgcggca ttgtgaaagt
     1261 aggcagcgaa gaatggcgtg ccgaactcgc acagccggat agcgcggcgc gagaaattgg
     1321 cgcgcacgtt caggtcgttt cggtcgaagg cgtaacgctc agagttcagt gaggtcatcc
     1381 gtttatggac aatccctttg ccacattttt gccgatcctg cttatcgcgc tgctgctggt
     1441 cggggtgctc cttctggtcc gcgccgcgcg catcgtcaac caatacgaaa aaggcatcat
     1501 catgcgcttg ggaaagtttc acggcctggc cccttcggga cttacgatca tcatgcccct
     1561 caccgatagc ctcattcgcg tggacatgcg cgaacaggtc atcagcgttc ctccacaaaa
     1621 attgattacc aaagacaacg tcaccgtcga agtcgacgcc gtcgtgtatt acaaagtcgt
     1681 cgatccggtg aagtcgcaat tcgaagtgca ggatttcggc tacgcctgca ccactctcgc
     1741 gcaaaccaat ttgcgcaact taatcggcga ccgcacgctc gacgaaacgt tggtcgctcg
     1801 cgacatgatc aataccaact tgcgccatgt gctcgatgaa gccaccaacg gctggggcgt
     1861 gaaagttacc cgcgtcgaag tgcagaaaat cgatccgccg cgcgacatca ccgaagccat
     1921 gtcacgccag atgaaagccg agcgcgacaa gcgcgccgcc gtgctcgaag ccgacggtgt
     1981 gaagcaaagc cagatccttc aggccgaagg tttcaagcag tccgaaatct tgaaagccga
     2041 gggcgacgcc caagctcgta tcactcgggc caacgccgaa gctgaagcga tccgcctggt
     2101 ttccaccgcc gccgaaacct tcttcaagga tcgcgccgaa gccatgcgcc gcctcgaagt
     2161 gttgagcagc actttggtcc acaacacgaa gtacatcgtc ccgtcgaacg ccggcctggt
     2221 gaatgtcctc ggtctcgacg gcgccatcgg tgccgccgcc agcgctgtcg ctcctccgaa
     2281 accgaaaggc tgagcactgt aactgacaga ttcttaatga cttaattgtc tcgcggggct
     2341 ttttacaact ttttacaatc gtttgcgttc gtttgacaca tccacctttt aaccccgtat
     2401 agttcactca cgcggagggt ttgcaaactt cgccgggacc gaaaatgaac gcaactcctg
     2461 ccaagaaaaa gcggctaacc gaccggacac gtatgctgct gacgctggag ctggcgattg
     2521 tcttgccagc cgcttcactg atggccttca gcgtgtggaa cctgcaccag attcagcgcg
     2581 acaaagccat cgaggccgcc attcaacgcg atttcacctc ggttctga
//
LOCUS       DASTPM010000469         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_708845, whole genome shotgun sequence.
ACCESSION   DASTPM010000469 DASTPM010000000
VERSION     DASTPM010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708845"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFR42_08325"
     CDS             <1..930
                     /locus_tag="VFR42_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0369199.1"
                     /translation="GWVTNIFQRGAASMGRLNYILTAKPSIDDSHATIAADRKPDGEI
                     EFRGLTFTYPTSLAGGDSAKAKTNGSGPSKNGSPPVLRDIDLKIPAGSTLAIVGPTGS
                     GKTTLAALIARLWEAPDGQLYLDGKPLREWPLASLRKSIGFVPQDTYLFGETIGGNIA
                     FGLPELRDEMVQEAAAIANLDSDVNDFPKRYETMVGERGITLSGGQKQRTAIARAVVR
                     DPRILILDDALSSVDTQTEERILSRLKEIMAGRTTILISHRTSTVRDADQIVVLLDGK
                     IAERGTHDELLAAGGYYADLYHKQLLEEELERA"
     gene            973..2859
                     /locus_tag="VFR42_08330"
     CDS             973..2859
                     /locus_tag="VFR42_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0369200.1"
                     /translation="MSNLHEEEALGKAYDSRLMRRLLQYMAPYKWWVVLALGLVAVVT
                     PLELAPPILFRNAIDRYFVPAMNRNIVENVAWNGILVISGIYLLVLVFDFLAQYAQIR
                     IMQRVGQQTMYDMRRGIFAHLQRLPMSYFDRNPVGRLVTRVTTDVDALNDLFAAGVVT
                     MINDLFLLVVMAGLLFKIDWRLALDTLGVLPAILIVTFVFRHYVREANRKIRTAIARI
                     NAFLQEYISGMSVVQLFNREHKAMQEFERRNKDNMLAWRDAILAYALFYPAVEFLSFA
                     TIALIFWAGGNRILHDGLTLGVLTAFTMYAQRFFRPIQDLSEKFNILQSAMAASERIF
                     KLLDEPITIQSAENCRRLAAARGEIEFRNVWFSYRNVAEPADEDWVLRDVSFRIQPGQ
                     TFAIVGHTGAGKTTLISLLLRFYDIQRGQILLDGVDIRLLDLQDLRRHFGIVLQDPFL
                     FSGTIESNIRLGTPGISHEDVERAIEKIGLADFVHSLNEGTATPVNERGSTLSVGQRQ
                     LINFARALAHNPRFLILDEATSSVDTKTELLIREALDKLLTGRTALVIAHRLSTIQYA
                     DRILVFHKGRLREQGAHQELLALRGIYYRLYQLQYKEQELHLPQANGSAPVPSFPAPL
                     PAND"
     gene            2873..4009
                     /gene="lpxB"
                     /locus_tag="VFR42_08335"
     CDS             2873..4009
                     /gene="lpxB"
                     /locus_tag="VFR42_08335"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU0369201.1"
                     /translation="MPAPQILLSAGEASGDMYAARLATALEARTHATIFGMGGDQMHA
                     AGVDVITPYSEVSVLGITEILSHLPQLVRAMRKLVNAARERKPELAILTDFPGFHLRL
                     ARKLKPLGVRNVYYVCPQFWAWRPWRVRIVRRRFALALCIFPFEEKFFGDAGVPVKFI
                     GHPLVGKVAPSKTRERFFAQHGLDTALPLVTILPGSRSSELTRHLSLLREACVKIHSA
                     MPVQFVVAAAPGRAASLERVWPGNLQVTVVEGQTYDALAHASASVVSSGTATVEAALL
                     DAPMVVVYRVTPLTALLAKPLVRTKHFSMVNLIAGREVVPELIQKDFTPDRVAEQMLR
                     LLREPTVAATMRANLAEVRRRLGPPGAVERAADAIAALLTQPAA"
     gene            4107..>4458
                     /locus_tag="VFR42_08340"
     CDS             4107..>4458
                     /locus_tag="VFR42_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369202.1"
                     /translation="MLLLCQASRLPRLGLLLVAAFALLLPAAGSAQDVSKENFLAEHY
                     DVAASIEPASQSLSAVAKVDFRAREASSIVRVELHPNLNVTKVESSAGKAVNFDRDAL
                     NPLLLTVNLPAPVAA"
BASE COUNT          880 a         1335 c         1332 g          911 t
ORIGIN      
        1 ggttgggtga ccaatatttt tcagcgcggc gcggcctcca tgggccgctt gaactacatt
       61 ttgactgcga agccgtcgat cgatgattcg cacgcgacga ttgccgcgga tcgcaagccc
      121 gacggggaaa tcgagtttcg cgggctgacg ttcacttatc cgacgagttt ggcgggcggc
      181 gactccgcaa aggcaaagac caacggaagc ggacccagca aaaacggctc gcctccggtg
      241 ttgcgcgata ttgatctgaa gattccggca ggctcgacgc tggcgattgt gggcccgacg
      301 gggagcggca aaacgacgct ggcggcgctg attgcgcgct tgtgggaagc cccggacggg
      361 caactatatc tggatggaaa accgttgcgc gagtggccgt tggcttccct gcgcaagtcg
      421 atcggcttcg tgccgcagga tacctatctt tttggtgaga ccattggcgg caacatcgcg
      481 tttggcttgc cggaactgag agacgagatg gtgcaagagg cggcggcgat cgcgaatctg
      541 gatagcgacg tcaacgattt tcccaaacgc tacgaaacga tggttggcga acgtggcatc
      601 acgctttcgg gcgggcagaa acagcgcacg gcaattgcgc gagcggtagt gcgcgatccg
      661 cgtattttga ttttggacga cgcactgtcc agcgtggaca cgcagacgga agaacgcatt
      721 ctaagccgat tgaaagaaat catggcgggg cggacgacga tcctaatttc gcatcgcact
      781 tcgacggtgc gcgacgcgga ccagatcgta gttttactcg acggaaaaat cgcggagcgc
      841 ggtacccacg atgaattgct ggcggccggc ggctactacg cggaccttta tcacaagcaa
      901 ttgctggaag aagagttgga acgcgcctga gcggcgcctg acggacttga tcagcttgat
      961 gaaaaagatc gcatgagcaa tttgcacgaa gaagaggcac tcggaaaagc gtacgactcg
     1021 cggctgatgc ggcgcctgct gcagtacatg gcgccgtata agtggtgggt ggtgctcgcg
     1081 ctggggctgg tggcggtggt gacgccgctc gaactggcgc cgccgattct tttccgaaat
     1141 gcgatcgacc ggtattttgt gccggcgatg aatcgcaaca tcgttgagaa tgtggcgtgg
     1201 aacggcatcc tggtcatttc cggaatctac ctgctcgtgc tggtcttcga ttttctggcg
     1261 cagtacgcgc agatccgcat catgcagcgc gtcgggcagc agaccatgta cgacatgcgg
     1321 cgcggcattt ttgcgcactt gcaacggctg ccgatgagct atttcgaccg caaccccgtg
     1381 ggccgtttgg tcactcgcgt caccacggac gtggatgctt tgaacgacct gttcgcggcc
     1441 ggcgtcgtca ccatgattaa cgatctgttt ctgctcgtag tcatggccgg gctgctgttc
     1501 aaaattgact ggcggctggc gctggacact ctgggcgtcc tacccgcgat cttgatcgtg
     1561 acattcgtgt tccgccacta cgtgcgtgag gccaaccgca aaatacgcac ggcgattgcc
     1621 cgcatcaacg cgtttctgca ggaatacatt tccggcatga gcgtggtgca gctcttcaac
     1681 cgcgaacaca aagcgatgca ggagtttgag cggcgcaaca aggacaacat gctggcgtgg
     1741 cgcgacgcga ttttggcgta tgcgctcttt tatcctgcgg ttgagttctt aagcttcgcg
     1801 accattgcgc tgattttctg ggcgggcggc aaccgcatcc tccacgatgg actgacgctg
     1861 ggcgttctga ccgccttcac catgtatgcg cagcggtttt tccggccgat tcaggatctg
     1921 agcgagaaat tcaatatttt gcagtcggcg atggcggcca gcgaacgcat tttcaagtta
     1981 ctggatgagc cgatcacgat tcaatcagcc gaaaattgcc ggcggctcgc ggccgcgcgc
     2041 ggcgaaattg aatttcgcaa cgtgtggttc agctaccgta acgttgcgga gcctgcagac
     2101 gaagattggg tcctgcgcga cgtatcgttc cgcatccagc cgggccaaac tttcgcgatt
     2161 gtcggccaca ccggcgcggg caagaccacg ctcatttcgc tgctgctgcg cttttacgac
     2221 attcagcgcg gacagattct actcgacgga gtggatattc gcttgctcga cctgcaggat
     2281 ttgcggcgcc actttggcat cgtgctgcag gatccgttcc tcttcagcgg cacgattgaa
     2341 tcgaacatcc gtctcggcac gccgggcatt tcgcacgaag atgtcgagcg cgccattgag
     2401 aaaatcggct tggcggattt tgtgcacagt ttgaacgaag gcaccgccac tccggtgaac
     2461 gaacgcggct cgacgttatc ggtcgggcag cggcaactga tcaactttgc gcgagcgttg
     2521 gcgcacaatc cacggtttct gatcctcgac gaagccacgt ccagcgtgga caccaaaact
     2581 gagctgctga ttcgcgaggc gctcgacaaa ttgcttacgg gccgtaccgc tctcgtgatc
     2641 gcgcaccggc tgagcaccat tcaatacgcc gaccgcattc tcgtctttca caagggccgg
     2701 ctgcgcgaac aaggcgcgca ccaggaactg ctcgccctgc gcggcattta ctaccgcctg
     2761 taccagctgc aatacaaaga gcaagagctg catttgccgc aagccaacgg ctcagcgccg
     2821 gtgccgtcct tccctgctcc actgcctgcg aacgactgaa caccgcccgc cgatgcccgc
     2881 cccacaaatc cttttatccg ccggcgaagc cagcggcgac atgtacgccg cgcgcctggc
     2941 gacagcgcta gaagcgcgca ctcacgcaac gatcttcggc atgggcggcg accagatgca
     3001 cgccgctgga gtggacgtca tcacgcccta ctccgaagtc tcggtgctcg gcatcacgga
     3061 aattctcagc catttgccgc agctggttcg tgcgatgcgc aaactggtca acgccgctcg
     3121 cgaacgcaag cccgaactcg cgattctcac cgacttccct ggttttcact tacggttggc
     3181 gcgcaagttg aaaccgctcg gcgtgcgcaa cgtctactac gtctgcccgc aattctgggc
     3241 atggcggccc tggcgagtac gcatcgtgcg gcggcgattt gcgttggcgt tgtgcatttt
     3301 tccgttcgag gaaaagtttt ttggtgacgc cggcgtgccg gtaaagttca tcggccatcc
     3361 attggttggc aaggtagcgc cctcaaaaac gcgcgagcga ttctttgcgc aacacggact
     3421 ggacacagcg cttccgctgg tcaccatcct gccgggcagc cgcagttccg aactgacccg
     3481 ccacttgtca cttttgcgtg aagcttgcgt caaaattcac agtgcgatgc cggttcaatt
     3541 tgtggtggca gccgcacccg gccgggccgc aagccttgag cgagtctggc caggcaacct
     3601 ccaggtcaca gtcgtggaag gccaaacata cgacgcgctg gcccacgcca gtgccagcgt
     3661 agtttcaagc ggtactgcca cagttgaagc cgcgctgctg gacgcgccca tggtcgtggt
     3721 ttatcgagta acaccgctca ccgcgctgct ggccaagccg ttggtgcgca caaagcactt
     3781 cagcatggtg aatttgatcg ctggccgcga agtcgtccca gagctcatcc agaaggattt
     3841 cacgccagac cgggtcgccg agcagatgct acgcctcttg cgagagccga cggtggcagc
     3901 cacgatgcgc gccaacctag cggaggtacg ccgccggtta ggcccgccag gagcggtcga
     3961 gcgtgccgcc gacgccatag cggccctgct gacccaaccc gccgcctagg gtcactggct
     4021 tacggccaag ctttctggca acgcttagtg acccgctgct atcctataga gggagcctta
     4081 ctgctgctcc caaggaatca gacctgatgc tccttctctg ccaagcttct cgcctgccca
     4141 ggttgggcct gctgctcgtc gcagcttttg ccttgctcct gccggcagcc ggatcggcgc
     4201 aggacgtcag caaagagaac tttctcgccg aacactacga cgtcgccgca agcattgagc
     4261 cggccagcca gtcgttgtct gccgtagcca aagtggattt tcgcgctcgc gaagcctcca
     4321 gcatcgttcg cgtcgagctg catcccaatc taaacgtgac gaaggttgag agctccgccg
     4381 gcaaagcagt gaactttgac cgcgatgcgc tgaacccgct cctgctgact gtcaacctgc
     4441 ctgcaccggt ggccgcca
//
LOCUS       DASTPM010000470         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1170284, whole genome shotgun sequence.
ACCESSION   DASTPM010000470 DASTPM010000000
VERSION     DASTPM010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170284"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1249)
                     /locus_tag="VFR42_08345"
     CDS             complement(44..1249)
                     /locus_tag="VFR42_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0369203.1"
                     /translation="MPDPVRTDAKPANHWLNRTVLGVGLTSLFSDWSHETATAILPTF
                     LAVIGAGPAWLGIIEGLADGLSSLAKLASGHYADRLQHRKPLAVFGYAFTAIATASFG
                     FATHALQILAARAAAWLGRGVRSPAKKALLAADVPPGAYGRAFGLERFMDTLGAILGP
                     LTALWLLEATNHAYRKVFFYTLIPGLIAVACFWLLVRERPIAVKEKKTFLHGLQTLPA
                     SFRKFLLGVGIFGAGDFSHTLLILYATKMLADQHGMARAASLAVVLYTIHNVFYAASA
                     YLSGWLSDHVPHRRAVLAAGYGLAVVTALLLRTGAHSLWLLGVISALAGFYIGTEEAL
                     EDSVTAEIVAREQHGLAFGTLAAVNAVGDFLSSLIVGALWSAFGVGAAFGFSAVLFAA
                     GAVLIVRLK"
     gene            1426..>3476
                     /locus_tag="VFR42_08350"
     CDS             1426..>3476
                     /locus_tag="VFR42_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0369204.1"
                     /translation="MGEGLAVQPTKLSKTIATDRALQRRAEHTAVKVEEVLNGFTERD
                     FGGEILTAVRRFPARAADFAEWPEWVVERLREVYRAKEIARPYSHQAAAAQAVHAGKN
                     VVIVTPTASGKTLCYNLAVIDAILKNEDARALYLFPTKALAQDQLAELYDLNQRLEKI
                     EKKASTSLRSSGQAESGRYPGEEKRPSEGGCYNRETIPAKAFGVFTYDGDTPSDARKA
                     IREKGHIVLTNPDMLHTGILPHHTKWTRLFENLKFIVIDELHSYRGVFGSHLCNVLRR
                     LRRVAKFYGSSPQFICCSATIANPGELAERLIEERAEVLSANGAPAGEKTFVFYNPPV
                     VNRALGIRRSYINESSRVAQEFLKRDLQTMVFANSRLHTEILLTYLQQANPHFPSSAE
                     PIRGYRGGYLPGERREIERGLREGRIRGVVSTSALELGIDVGSLDATVMAGYPGTIAA
                     TWQRAGRAGRRSGSSCAVLVASSAPLDQFIVRHPDYFFGNTPEHGFIQPDNLEILINH
                     LKCAAFELPLGPEEKFGEADLPDLCARLAEAGYLHRAGRHYHWTQEAYPADTVSLRSV
                     TSDNFVIIDITGAPAVIGEVDFPSALTTVHEKAIYLHGGEQYHVEHLDFTERKAFVKK
                     VDVDYYTDAVRYTQVRVLEVAKEARANVERPGIAASKSHGDVLVRSQVVGFKKIKFFT
                     N"
BASE COUNT          766 a          836 c         1264 g          610 t
ORIGIN      
        1 aaagcggcca gaggcagcag cgacatcgtc gcgtccaggc acgtcatttc agtcgaacaa
       61 tgagaacggc gccggcggcg aagagtacgg cggagaagcc gaaggctgcg ccgacgccga
      121 aagcggacca gagtgcgccg acgattaggc tggagagaaa atcgccaacg gcgtttaccg
      181 cggcgagagt gccgaaggcc aggccgtgct gttcgcgggc aacgatttca gcggtgacgg
      241 aatcttccag ggcttcttcg gtgccgatgt agaaacctgc cagagcggag atgacgccga
      301 gcagccagag cgaatgcgcg ccggtgcgaa gcagcagagc ggtgacgacg gcgagtccat
      361 agcctgcggc gagaacggca cggcgatgcg gaacgtgatc gctgagccag ccgctgaggt
      421 aggcggaggc ggcgtaaaaa acgttgtgaa tggtgtagag gacgacggcg aggctggcgg
      481 cgcgagccat gccgtgctgg tcggcgagca tcttggttgc gtaaagaatc aggagagtgt
      541 gggagaaatc gcctgcgccg aaaatgccaa cccccagaag gaatttgcgg aaggatgcgg
      601 ggagagtttg caggccatgc agaaaagttt tcttttcttt aacggcgatg gggcgttcgc
      661 gaacgaggag ccagaagcag gctacggcga tcaggccggg aatgagcgtg tagaaaaaaa
      721 ccttgcggta ggcgtgattg gtggcttcga gaagccagag cgcggtgagc ggaccgagga
      781 tggcgccgag cgtgtccatg aaacgctcga ggccgaaggc gcggccgtag gcgccgggcg
      841 ggacgtccgc ggcgaggaga gcttttttgg cgggcgagcg gacgccgcga ccgagccagg
      901 cggcggcgcg ggcggccagg atctgcagcg catgggtggc gaagccgaac gacgcggtgg
      961 cgatggcggt gaaggcgtaa ccgaaaactg cgagcggctt gcggtgctgg aggcggtcgg
     1021 cgtaatggcc agaggcaagc ttggcgaggc tggagaggcc gtcggcgagg ccttcgatga
     1081 tgccgagcca ggcggggccg gctccaatga cggcgaggaa agtgggcagt atggcggtgg
     1141 cggtttcgtg gctccagtcg gagaagagcg atgtgaggcc gacgccgagg acggtgcggt
     1201 taagccagtg atttgcgggc ttggcatcgg ttctgaccgg gtcgggcatg agagagagtc
     1261 tacaggcgac tgtgcagagt tgaacagcgg acgtttccgt gtgtgaacaa aaagcgaaaa
     1321 tgaggattgc gttgggggta tcgcgatgct agtctcagcg aataaaaggc gaaccgtaga
     1381 acggcgggtg ggacgcggga taagccgccg ccacaaggag cagtaatggg cgaagggttg
     1441 gcggttcaac cgacaaaatt atcgaagacg attgcgaccg atcgagcgct gcagcggcgc
     1501 gcggaacaca ccgctgtgaa agtggaagag gtgctgaacg gtttcacgga gcgtgatttt
     1561 ggcggagaaa tacttaccgc cgtgcggcgt ttcccagcgc gcgcggcgga ttttgcggag
     1621 tggccggaat gggtagtcga gcggctgcgc gaagtgtatc gcgcgaaaga aattgcgcgg
     1681 ccgtattcgc atcaggccgc ggcggcgcaa gcggtgcatg cgggcaagaa tgtggtgatc
     1741 gtgacgccga cggcttcggg taagacgctg tgctacaacc tggcggtgat cgacgcgatt
     1801 ttgaagaatg aggatgcgcg ggcgttgtat ttgtttccga cgaaggcgct ggcgcaggat
     1861 caattggcgg aattgtatga cctgaatcag cggttggaga agattgaaaa aaaggcgtcc
     1921 acttcccttc gcagctcagg gcaagcagaa agcggccgct acccaggcga agagaaaagg
     1981 ccgtctgaag gtggctgcta caaccgagaa acaataccgg cgaaggcgtt tggagtgttt
     2041 acctacgacg gggacacgcc aagcgatgcg cggaaggcga tacgcgagaa ggggcacatc
     2101 gtcctgacca atccggacat gctgcacacg gggattttgc cgcaccacac aaagtggacg
     2161 cggctgtttg aaaacttgaa attcattgtg atcgacgagc tgcattcgta tcgcggggtt
     2221 tttgggagcc atttgtgcaa cgtgttgcga cgcttgcgac gggtcgcgaa gttttatggg
     2281 agctcgccgc aatttatttg ctgttcggcg acgattgcga atccgggaga gctggcggaa
     2341 cggctgattg aagagcgggc ggaagtgctg agtgcgaacg gcgcgccggc tggagaaaag
     2401 acatttgttt tttacaatcc gccggtggtg aaccgcgcgc tggggattcg gcgcagctac
     2461 atcaatgaaa gctcgcgggt ggcgcaggaa tttctgaagc gcgatttgca gaccatggta
     2521 ttcgcgaatt cgcggctgca tacggagatt ttgctgacgt atctgcagca ggcgaatcca
     2581 cactttccga gttcagccga gccgattcgc ggctatcgtg gcggctatct gccaggcgag
     2641 cggcgcgaga ttgagcgcgg cctgcgcgaa ggacggattc gcggtgtggt gagcaccagc
     2701 gcgctggaat tgggcatcga cgtcggctcg ctggatgcga cagtcatggc gggttatccc
     2761 ggaacaatcg ccgccacgtg gcagcgggcc gggcgcgcgg ggcggcggag cggaagttcg
     2821 tgcgcggtgc tggtggcgtc ctcggcgccg ctcgatcaat ttatcgttcg gcatccggac
     2881 tatttttttg gcaacacgcc ggagcacggg ttcattcagc ccgacaatct tgagattttg
     2941 atcaaccacc tgaagtgtgc ggcgtttgag ctaccgctgg ggccggagga gaaattcggc
     3001 gaagcggact tgccggatct gtgcgcgcgg ttggctgaag caggctatct gcatcgcgcg
     3061 gggaggcact atcactggac gcaggaagca tatccagcgg atacggtgag cctgcgctca
     3121 gtgaccagcg acaatttcgt gatcattgat attacgggcg cgccggcggt gatcggcgaa
     3181 gtggattttc cgagcgcgct gacgactgta cacgagaaag cgatttatct gcacggcggc
     3241 gagcaatatc acgtagagca tctggacttc acagaacgca aggcgttcgt gaaaaaagtg
     3301 gacgtggact actacacgga tgcagtgcgc tatacacagg tgcgcgtgct ggaagttgcg
     3361 aaagaagcga gggcgaacgt ggaacggccg gggatcgcgg caagcaaatc acatggagac
     3421 gtgctggtgc gttcgcaggt ggtaggcttc aagaagatca aattctttac caacga
//
LOCUS       DASTPM010000471         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_160678, whole genome shotgun sequence.
ACCESSION   DASTPM010000471 DASTPM010000000
VERSION     DASTPM010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160678"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFR42_08355"
     CDS             complement(<1..217)
                     /locus_tag="VFR42_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEU0369205.1"
                     /translation="MVPPLMQHALSEASLPRGFRFAATACGLKKTGALDFAIISSDAP
                     ASAAGVFTQNLVVAAPVLISKAHLRASK"
     gene            complement(329..1771)
                     /gene="argH"
                     /locus_tag="VFR42_08360"
     CDS             complement(329..1771)
                     /gene="argH"
                     /locus_tag="VFR42_08360"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU0369206.1"
                     /translation="MTQPTQRKDDRLWGGRFEREPDKTFDAFQRSFSVDRRLLPYEIA
                     VDRAWAKALQAIGIFTAAELHDTLLALDNLSAQIAGDPAFLDSKQVEDVHHFVESALV
                     HALGPPGWKLHTGRSRNELVATDFRLFVMQAAARTQSAIVDLQSAFLEQAKANLGVTM
                     AGMTHMQHAQPILLSHFLLAHAETFSRDVTRLQSAAASADACPMGSGALAGNSFGIDR
                     HAVARELGFSRITANSLDAVSDRDFAVDYLFALTGIATHLSRLAEDFVIFASQEFAYV
                     ILPDEYSTGSSLMPQKKNPDAWELIRGKSGRITGALLAVLTTLKGLPSSYQRDLQEDK
                     EPLFSAHDQIAGMLAVATGAVAATKFNADRLKAAASNPLLLATEAADYLARKGMPFRQ
                     AHDVIGKVLREAEKQNVPWTSLPLAALKKIAPEFDADYSASLTVEAALSTKKVPGGTA
                     PESVRAAISDLQNRIAKLSSTLSAKSGAKP"
     gene            complement(1946..3172)
                     /locus_tag="VFR42_08365"
     CDS             complement(1946..3172)
                     /locus_tag="VFR42_08365"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU0369207.1"
                     /translation="MNKASNHPKPKKVVLAYSGGLDTSIIIPWLKENYQCEVIAMIGD
                     VGQQEDLEAARKKAMATGASTAYVEDLREEFVRDYIWPTLRAGAVYEHTYLLGTSMAR
                     PILGKRQAEIALKLGADGLSHGCTGKGNDQVRFELAYKAIAPNLQIIAPWREWDIDSR
                     EDAIAYAQKHNIPVEQSKKNIYSRDRNIWHLSHEGGELEDPANSPNEAMWQWISSPEK
                     APDKAAEVEIGFEAGLPVSVNGAKANPIDLLNTLNEIAAKHGIGRTDIVENRLVGMKS
                     RGAYETPGGTLLVTAHRELEALTVDRETAHFQQALSLRYAELVYYGLWFSPLRAALDG
                     FFSVAQQRVTGAVGLRLWKGTLTVTKRVSPFSLYRTDLASFTMGRYNPKDAEGFINLF
                     ALPVTVRPKAKAESKS"
     gene            complement(3261..4211)
                     /gene="argF"
                     /locus_tag="VFR42_08370"
     CDS             complement(3261..4211)
                     /gene="argF"
                     /locus_tag="VFR42_08370"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0369208.1"
                     /translation="MTTTTLVAPISLSNDLLTGAEWSPSHTRDLLHLSADIKARPGRY
                     ASTLQGKFIALIFEKPSLRTRVTFEVGIQSMGGSVIFLDHTQASASLGARESIADVAR
                     NLDRWVQGIVARVYHQRVLEEMAANADIPVINALSDKFHPCQALADFFTLEEKFGALR
                     GFKLTYIGDGNNVCNSLIYLAARLGVHLRVATPPDYAPAAEVVADGKRIARETKAKID
                     LLTDPREAVSGAQAVYTDSWTSMGFEEEGEVRNAVFQPYQVNAELMSNAAPDAVFMHC
                     LPAHRGLEVTPEILDGPHSVVLDQSENRMYVQKAILHTLF"
     gene            complement(4216..>4455)
                     /locus_tag="VFR42_08375"
     CDS             complement(4216..>4455)
                     /locus_tag="VFR42_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369209.1"
                     /translation="ELSIDGNPFAAEALRQGLIINCTHDFTLRLLPPFIITRAQVREF
                     LRLFELVLAKTPKSTSAAPAQSAANPGRLAQSAAR"
BASE COUNT          827 a         1064 c         1635 g          929 t
ORIGIN      
        1 cctttgaagc gcgcagatgc gctttggaaa tcagcacggg agcggcaacg accagatttt
       61 gcgtgaacac accggcggca gaggcggggg cgtcactgga gatgatggcg aaatcgagcg
      121 cgccggtttt cttcaagccg caggcggtgg cggcgaagcg gaagccgcgg ggcagcgagg
      181 cttccgaaag ggcgtgctgc attagcgggg ggaccatgga gtgagcgcga tgctcacgga
      241 agggacctcc ggccctccgc ggaagaggcg ctttgcaaga gggcgcgtat ctccgagagg
      301 gtttgttgta atcgaacggc ttgtgtgctc atggcttagc tcctgatttg gcggaaagag
      361 tggaagagag tttagcaatg cggttctgca aatcggaaat ggcggcgcgg acggactcgg
      421 gcgcggtgcc gccgggaact tttttggtgg aaagcgcggc ctcgacggtg aggctggcgg
      481 aataatcggc gtcgaattcg ggggcgattt ttttcagcgc tgcgaggggc aacgaagtcc
      541 atggcacatt ttgtttttcg gcttcgcgca agactttgcc gatgacgtcg tgggcctgac
      601 ggaagggcat gcctttgcgg gcgagatagt cggcggcttc ggtggcaagg agaaggggat
      661 tggacgcggc ggctttgagg cggtcggcgt tgaactttgt ggccgcgacg gcgccggtgg
      721 ctacggccag catgccggcg atttgatcgt gcgcggagaa gagcggttct ttgtcttcct
      781 gcaagtcgcg ctggtagctg ctgggcaggc ccttcagcgt tgtgagcacg gccagcagcg
      841 cgccggtgat gcggccggat ttgccgcgaa tcagttccca ggcgtcggga tttttctttt
      901 gcggcatgag gctgctgccg gtggaatatt cgtcggggag aatgacatag gcaaattcct
      961 gggacgcgaa aatcacgaag tcttccgcaa gcctcgagag atgggtggcg attcccgtga
     1021 gcgcgaaaag atagtcgacc gcgaaatcgc gatcgctgac ggcgtcgagg ctgttggcgg
     1081 tgatgcggga gaagccgagt tcgcgcgcga cagcgtggcg gtcgattccg aaactgttgc
     1141 cggccagcgc tccggagccc atgggacacg cgtcggcgct ggcggcggcg gattgcaagc
     1201 gagtgacgtc gcgggaaaag gtttcggcat gagccagcaa aaaatgggaa agcaaaatcg
     1261 gctgggcgtg ctgcatgtgg gtcatgccag ccatggtcac gccgagattg gctttggctt
     1321 gctcgagaaa ggcgctttgt aaatcgacga tggcggattg ggtgcgggcg gcggcttgca
     1381 tgacgaagag gcggaagtcg gtggcgacga gttcgttgcg gctgcggccg gtgtgcagct
     1441 tccagccggg cgggccgagt gcatggacca gagcgctctc gacgaaatgg tggacgtctt
     1501 cgacttgctt gctgtccagg aaggcagggt cgccggcgat ctgcgcagag agattgtcga
     1561 gcgcaagcag ggtgtcgtga agttcggcgg cggtgaaaat gccgatggcc tggagagcct
     1621 tggcccaggc gcgatcgacc gcgatttcgt agggcagcaa gcggcgatcg acggagaagg
     1681 aacgctggaa ggcgtcgaag gttttgtcgg gctcgcgttc gaagcggccg ccccagaggc
     1741 gatcgtcttt gcgttgggtt ggctgggtca tgagatagcg cctacgtctt agaattcgcc
     1801 gatgcttttg ttgctgggcc agcggttgag gtccttcggc taccctcatc gggtagcctc
     1861 aggatgacag tcgtgtgaag gtgacgcgcg tgaaggtgac gcgagccagc ctctgacacg
     1921 ttatcggaat tggcgcgggc ggaggtcacg acttgctttc ggctttggct ttggggcgca
     1981 ccgttactgg gagcgcgaaa agatttatga agccttcggc gtctttgggg ttgtagcggc
     2041 ccatggtgaa gctggccagg tcggtgcgat agagagagaa aggagagacg cgcttggtga
     2101 cggtgagcgt gcctttccag agtcttaagc cgacggcgcc ggtgacgcgt tgctgggcga
     2161 cgctgaagaa accgtcgagc gctgcgcgca ggggagaaaa ccacaaacca tagtagacaa
     2221 gttccgcata gcgcagcgaa agggcttgct ggaaatgcgc tgtttcgcga tcgacggtga
     2281 gggcttcgag ttcgcgatgc gcggtgacca gcaaagtgcc gccgggagtt tcgtaggcgc
     2341 cgcgagattt catgccgacc aggcgatttt ccacgatgtc agtgcggccg atgccgtgct
     2401 tggcggcgat ttcgttgagg gtgttcagca aatcgatggg attggctttt gcgccgttca
     2461 cggaaacggg gaggccggct tcgaaaccga tttcaacttc ggcggctttg tcgggcgcct
     2521 tttccggcga agaaatccat tgccacatgg cttcgttggg agaattggcg ggatcttcga
     2581 gctcgccgcc ttcgtggctg aggtgccaga tattgcgatc gcggctgtag atattctttt
     2641 tgctttgctc gacggggatg ttgtgctttt gggcgtaggc gatggcgtct tcgcgggagt
     2701 cgatgtccca ctcgcgccag ggggcgatga tctggagatt gggagcgatg gctttgtagg
     2761 ccagctcgaa acggacctgg tcgttgcctt tgccggtgca gccgtgagag aggccgtcag
     2821 cgccgagttt cagtgcgatt tccgcctggc gtttgccgag gatgggccgg gccatggacg
     2881 tgccgaggag ataagtgtgc tcgtagacgg cgcccgcgcg cagggtgggc cagatgtaat
     2941 cgcgaacgaa ttcttcgcgg agatcctcga cgtaggcggt ggaggcgccg gtagccatgg
     3001 cttttttgcg ggcggcttcg aggtcttctt gttggcctac gtcgccgatc atggcgatta
     3061 cttcacactg ataattttct ttcagccaag ggatgatgat ggaggtgtcc aggccgccgg
     3121 aataggccag gactaccttt tttggttttg ggtggtttga tgctttgttc acaagtcctc
     3181 tctcgtttag ttcggcgcga attttttcga ccgtaaaagc ggcggcaaga ccgccgcact
     3241 ccacaaaaag ccgccgctat ctagaatagc gtgtgcagga tggctttctg cacgtacatg
     3301 cgattttcgg attggtccag gaccacggaa tgggggccat ctaaaatttc cggcgtgact
     3361 tccaggccgc ggtgggccgg gagacagtgc atgaagacgg cgtcgggcgc ggcgtttgac
     3421 atcaattcgg cgttgacttg atagggctgg aagacggcgt tgcgaacttc gccttcttct
     3481 tcgaagccca tgctggtcca ggaatcggtg tagacggctt gagcgccgga gacggcttcg
     3541 cgcgggtcgg tgagcaaatc gattttagct ttggtttcgc gggcgatgcg cttgccgtcc
     3601 gcaacgactt cggctgcggg agcgtagtcg ggcggagtgg ctacacgcag atgaacgccg
     3661 agacgcgcgg cgaggtagat aagcgaattg cagacgttgt tgccgtcgcc gatgtaggtg
     3721 agcttgaaac cacgcaaggc gccgaatttt tcttcgaggg tgaagaaatc ggcgagggcc
     3781 tggcagggat gaaatttgtc ggagagcgcg ttgattacgg gaatgtcggc attggcggcc
     3841 atttcctcca ggacgcgctg gtgataaacg cgggcgacga tgccttgcac ccagcgatcg
     3901 agattgcggg cgacatcggc gatggattcg cgagcgccga gagaggcaga ggcctgggtg
     3961 tggtcgagga aaatgacgga gccgcccatg ctttggatgc cgacttcgaa ggtgacgcgg
     4021 gtgcgtagcg agggcttctc gaaaatgagc gcgatgaact tgccctgcag cgtggaggcg
     4081 tagcggccgg ggcgggcttt gatgtcggcg gagagatgga gcaggtcgcg ggtgtgcgag
     4141 gggctccatt cggcgccggt aagaagatcg ttcgataaag aaataggagc gacgagagtg
     4201 gttgtggtca tcgtttcacc ttgccgcgga ttgcgcgagg cgccccggat tggcggcgga
     4261 ttgcgcggga gcggcggatg tagatttggg agtttttgcg agaacgagct cgaagagccg
     4321 gagaaattcg cgaacctgtg cgcgggtgat gatgaacgga ggcagcagtc gcagggtaaa
     4381 gtcgtgggtg caattgatga tcaggccctg gcggagggcc tcggcggcga agggattgcc
     4441 gtcgatggag agttc
//
LOCUS       DASTPM010000472         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_968167, whole genome shotgun sequence.
ACCESSION   DASTPM010000472 DASTPM010000000
VERSION     DASTPM010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968167"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2733)
                     /locus_tag="VFR42_08380"
     CDS             complement(<1..2733)
                     /locus_tag="VFR42_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEU0369210.1"
                     /translation="MSRVHRLGILLLLITAATLLAFALPSPHSRASSSTQKSQGSAKA
                     ESRPNESEAFRNNTLGVAYMNQQKFAEAQKYFEKALAADSHFAIALLNLGISHLSQQQ
                     LDPARQALEQAARLLPNDPYVWYNLGLVYKDTGEPEKGIDAFLHVTKLSPEADAHYFL
                     GYLYSQLQRYDEAVAEFQKALAAFPYHASSEFGIARAYQRKGDSDSARQHLARFQKIT
                     SEHLGTPFGAGYGDQGRFSLAELGRSGVTTAWPPIAVSYKQQPLVAAAAKLPGPSTGA
                     CFLDYDGDGKPDLFLVSATAEGTSRLLHNAGDGHFVDVTDTAGLALKGAGLGCAAGDF
                     DNDGHTDLAVCLHDGVHLLRNTGDGKFADVTETVGLRRQPGCVGVTFVDYDHDGDLDL
                     YVTMQPAAAASPEARPRNLLWRNNGNSTFTDISAETGLGIDATGAGLVTSDFNNDRAI
                     DFVFAGGPQGAAIYLNPREGKFSPLPAIDFTKENLPPAVGVVAFDFDKDGWMDLAFTH
                     AGAPGISLWRNIEGKRLERVKLPDFGWQRGWGISALDYDNDGWLDLVAAGETSSGGEL
                     RLLRNLGSSAFADVTREVHLDAVKLAQPRAIAVADTAGTGSPDLIVTQLGAPPLLLRN
                     EGANQHNWLRLDLKALNDNKSAIGTKVEIYAGPLYQKWEVQGASGYLGQNAPTLLAGL
                     GAEKNVEVVRLLWPTGVPQDEINLAARKEHALGELDRRGSSCPVLFAWNGREFEFIAD
                     MIGPGVVGHWVAPGERDVPDPDEYLKVNARSVRPRKGLLSFRFLEPMEETVYLDQVRL
                     LAVDHPAALDVYPNERFVSAPPFPEFRVIASRDGHPPLGAWDDRGNDVLSLIAKADRK
                     YVTNLEDMPFAGFAKLHWLELDLGSWDPAKPLRLIIDGYTDYFTATSMYAADQAG"
BASE COUNT          534 a          724 c         1052 g          566 t
ORIGIN      
        1 gccagcttga tcggcggcgt acatagaagt ggcggtgaag taatcggtgt agccgtcaat
       61 gatcaggcgc agcggtttgg cggggtccca ggaaccgaga tcgagttcca gccaatgaag
      121 ttttgcgaaa ccggcgaagg gcatgtcttc gaggttggtg acatatttgc ggtcggcttt
      181 ggcaatgagg gagagcacat cgttgccgcg atcatcccaa gcgcctagcg gcggatggcc
      241 gtcgcggctg gcgattacgc gaaattcggg gaatggcggg gcgctgacga agcgttcgtt
      301 gggataaacg tcgagcgcgg cgggatgatc aaccgcgagc aggcggacct gatccaagta
      361 gacggtttct tccatcggct cgaggaaacg gaagctgagc aggcctttgc gcgggcgcac
      421 gctgcgcgcg ttgaccttca aatattcgtc gggatcgggg acgtcgcgtt cgccgggggc
      481 gacccaatga cctacaacgc cgggaccgat catgtcggcg atgaattcga attcgcggcc
      541 gttccaggcg aacaagaccg ggcaggaact gccgcggcgg tcgagttcgc cgagggcgtg
      601 ctcttttctg gcggcgagat taatttcgtc ctgcgggacg ccggtgggcc agagcaggcg
      661 aacgacttca acattttttt cggcgccaag accggccagc agagtcggcg cgttttggcc
      721 gagatagccg gaagcgcctt gcacttccca cttttgatag agcgggccgg cgtagatttc
      781 gactttggtg ccgatagcgc ttttgttgtc gttgagggct ttgaggtcga ggcggagcca
      841 attgtgctgg ttggcgcctt cgtttcgaag caggagcggc ggagcgccga gttgcgtaac
      901 gataagatcg ggcgagccgg tgccggcggt gtcggcgacg gcgatggcgc gtggctgcgc
      961 gagcttgaca gcatcgagat ggacttcacg cgtgacatca gcgaaagcgg atgagccgag
     1021 attgcgcagc aggcggagtt ctccaccgga actggtttcg ccggcagcta cgagatcgag
     1081 ccagccgtca ttgtcataat cgagcgcgga aatgccccag ccgcgctgcc agccgaaatc
     1141 ggggagcttg acgcgctcga ggcgcttgcc ttcgatattg cgccataggc tgatgccggg
     1201 cgcgccggcg tgggtgaagg caaggtccat ccagccgtct ttatcgaaat cgaaagcgac
     1261 gacgccgacg gcggggggga gattttcttt ggtgaaatcg atggcgggca gcggagaaaa
     1321 ttttccttca cgcggattca ggtagattgc ggcgccttgc gggccgccgg cgaaaacgaa
     1381 atcgatggcg cgatcgttgt tgaagtcgga agtgaccagg ccggcgccgg tggcatcgat
     1441 gccgaggccg gtttcagcgg aaatgtcggt gaaggtgctg ttgccgttgt tgcgccacag
     1501 aagattgcgc ggccgggcct cgggcgaggc ggcagccgca ggttgcatgg tgacgtagag
     1561 atccagatcg ccgtcgtgat catagtcgac gaaggtgacg ccgacgcatc ctggctggcg
     1621 gcgaaggccg acggtttcgg tgacgtcggc gaatttgccg tcgccagtat tgcgcaggag
     1681 atggacgccg tcgtggaggc agacggcgag gtcggtgtgg ccgtcgttat cgaaatcgcc
     1741 ggcggcgcaa ccgaggccgg cgcctttgag ggcgaggccg gctgtgtcgg tgacatcaac
     1801 gaagtggcca tcgccggcgt tgtggagcag gcggctggtg ccttccgcgg tggcgctgac
     1861 taaaaacagg tcgggcttgc cgtcgccatc gtaatcgaga aaacacgcgc cggtgctggg
     1921 gccggggagt ttggcggcag cggccaccag cggctgctgc ttgtagctga ccgcgattgg
     1981 cggccaggcg gtggtgactc cgctgcggcc taattcggca agcgagaagc gaccctggtc
     2041 gccataaccg gcgccgaacg gagtgccaag atgctcgctg gtgattttct ggaagcgcgc
     2101 gagatgctgg cgggcggaat cggaatcgcc tttgcgctgg taggcgcggg cgatgccaaa
     2161 ctccgaggac gcgtgatacg gaaaagcggc gagagctttc tgaaattcgg cgacagcttc
     2221 atcgtagcgc tggagttggg aatagagata gccgaggaag tagtgggcgt cggcttccgg
     2281 ggaaagtttc gtgacgtgga ggaatgcgtc gatgcccttt tcgggctctc cggtgtcttt
     2341 gtaaaccagg ccgaggttgt accagacgta gggatcgttg gggagaaggc gcgcggcttg
     2401 ctcgagcgct tggcgagccg ggtccaattg ttgctggctg aggtgggaga tgccgaggtt
     2461 gaggagagcg atggcgaagt gggagtcggc ggccagggcc ttctcgaaat atttctgggc
     2521 ttcggcaaat ttctgctggt tcatgtaggc gacgccgagg gtgttgttgc ggaaggcttc
     2581 agattcgttc gggcgagact ctgctttggc ggagccctgc gatttctggg tgctgctgct
     2641 cgctcgcgag tgcggcgagg gaagcgcaaa ggccaagaga gtggccgcgg taatcaggag
     2701 cagcagaatt ccgaggcggt ggacacgtga catcaagttt tttcctgtac gaactgtccg
     2761 agcgcgggaa aaacggcacg cttcgagact tagggatttt cgcgggtgca tgaggcagcg
     2821 tcaagttgcg ttttacttgc gttcgaaatg gcgacgcctt cggatttctc ggcgtg
//
LOCUS       DASTPM010000473         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1227672, whole genome shotgun sequence.
ACCESSION   DASTPM010000473 DASTPM010000000
VERSION     DASTPM010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227672"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFR42_08385"
     CDS             complement(<1..604)
                     /locus_tag="VFR42_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369211.1"
                     /translation="MQTDSIENILVVDDEEAIREVVSTMLESKGYHCTAVSNGRAAQD
                     IVKRTTPDLVLSDMIMPEMDGIKLLEWLRQYDPEIPVIMVTAIHDISTALEAIRRGAY
                     DYILKPFEKDQLYLGVNRALQHRRLVAENRNYQRDLERQVEERTARLTIALAQLEQSY
                     DDTLEALGSALDLKDAETEGHCQRVTAFTISIAKAMPVPNA"
     gene            790..3930
                     /locus_tag="VFR42_08390"
     CDS             790..3930
                     /locus_tag="VFR42_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0369212.1"
                     /translation="MLETQGLSAVAFFEATAFIILLVLFVLLRKDHPSRYLNVWVAAW
                     ALLTIKAVLELAQLTVEFPQLRVLCVVLLLVVHLLFVKAVVQYAYGPRTSIFHLWPVT
                     GAVVLLGFYFESRPVVGTPHIGWFASAALAVTSLAAGWCLWNAKKKKLGHGARLLGGL
                     FFLDGIHGLDRFMWHQSPLFLQRVAFDHLLAVALGIAMVVLVLEEARARTEELHDKLN
                     RLSLLIAASTQTLTVREMLDRVLVNVVGSLGVSHGLVRLLQGEGNSAQLAVQAATGFD
                     ESYLQTHHVIPANEPWAQRVLKEDYTSAKIGEEADPRERQRMSATGVREIATIVLRGK
                     DRPLGILAVGCAQEMKFQPDEIDYLKNIGNLLGLTLQSALLFQQLDNVQRQWAFTFDS
                     IGDPIVVHDGEGRILRLNQRMAQLSGRELKSWIGRNIAELFSRKQADYTRCPYCEGAA
                     GEGDDPDPWLSGYFLTSNSPFVDSNGNQMGVIHVLKDISERKRAEEKYRTLISNVQEG
                     VFISTPQGRFLDFNDAFLRMTGYTSREELMNLDVQALYASPADRDRLRKLLADYGSVA
                     DFEFEIQRKDGETRTVLESSNAVKDSTGNIIAYQGFMLDITERKRAEHEIRRRNRELL
                     VLNSIATTLNESLDLSDSLHKTLRQICELFSLEASSLYLFDSGNEMLRRVAAFGHRSE
                     FARNFPPTRVQPELLQHIRAVRAMFLSIQGLPLPPIFRELQRAEQIVAAHIVILWSKD
                     KVIGGLVVGSRAPRDFSPGDVNLLIAVGSQISSAIERTLLYDQTRQAYDDLRRTQEQL
                     LHSEKMAAVGQLISGVAHELNNPLTAILGYSQLLTSCGQVGQQGLEYSEKLYKQAQRT
                     HRIVQNLLSFSRQHKPERVPVRLNQILEDTLALRDYDLRMGNIRLHLELAPDLPSAMA
                     DPHQLQQVFLNLVNNAVDAMLECSASGDLWVRTALENNKFILEFVDSGPGVKDGSRVF
                     DPFYTTKPVGKGTGLGLSICYGIITEHGGQIRVRNVPPRGACFTIELPGPSRSSGTES
                     SVLETVASGPGSTALN"
     gene            complement(3947..>4276)
                     /locus_tag="VFR42_08395"
     CDS             complement(3947..>4276)
                     /locus_tag="VFR42_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369213.1"
                     /translation="RRERRNVALLYLESLRSDFDQLLRIARVVALLSPEVSSSHEYER
                     LRLSILFRVRFQLAKLHFLFGDVAMLQLASLGQMVSSLAIRMETAMATLGERAALAAD
                     LALQSDQ"
BASE COUNT          864 a         1198 c         1297 g          917 t
ORIGIN      
        1 aggcattggg aaccggcatg gccttggcga tggaaatggt gaaggcagtg acgcgctggc
       61 agtggccttc ggtttcggcg tctttcaggt cgagagcgct gcccaatgct tccaaggtgt
      121 cgtcgtagga ctgttccaac tgagccagcg caatcgtgag acgcgccgta cgttcttcca
      181 cctggcgttc cagatcgcgc tggtaattac ggttctccgc gaccaggcgg cgatgttgca
      241 acgcgcggtt tacgccgagg tacagctggt ccttctcaaa aggtttgagg atgtagtcgt
      301 aagcgccgcg gcgaatggct tcgagagccg tggaaatatc gtggatggcg gtgaccataa
      361 tcaccggaat ttccgggtcg tattggcgga gccactcgag gagtttgatg ccgtccatct
      421 ccggcatgat catgtcgctg aggaccagat cgggagtggt gcgcttgacg atatcctgcg
      481 ccgcccggcc gttgctgacc gccgtgcagt gataaccctt ggactcgagc atggtggaaa
      541 cgacttcgcg aatggcttct tcgtcgtcga ctaccagaat gttttcgatg ctatccgttt
      601 gcacgtggct tcactccgca caccagtata cgagaggtca cagaaggccg gacgtaccct
      661 tgtccattca gaaaacagca tgttagcgtc aaaaaagtcg cgattcgcgc agctctgagc
      721 agccctttca gtaccgcggg gcgggtgtcc tccgggctaa tgcgcgtcca tgttgggagg
      781 cggcgcttcg tgctggaaac tcagggccta tctgccgtcg ccttctttga ggccaccgcc
      841 tttattattc tgctggtcct gtttgtgctc ctgcgtaagg accatcccag ccgctattta
      901 aatgtttggg ttgcagcctg ggcgctgctg accatcaaag cagtgctgga actggcgcag
      961 ctgactgttg aatttccgca actgcgggtc ctgtgcgtgg tcttgctgct ggtggtgcac
     1021 ctgcttttcg tgaaagcagt ggtgcagtat gcctacgggc cgcgcacctc gatctttcat
     1081 ctatggcccg ttaccggcgc tgtggttttg ctgggctttt actttgagag ccggccggtg
     1141 gtgggcacgc cccacattgg ctggtttgct tccgcagctt tggcggtcac ttccttggcg
     1201 gcgggctggt gcctgtggaa tgccaagaag aagaagcttg gccatggcgc caggcttctg
     1261 gggggattgt tcttcctgga tggcattcac ggtctggatc gtttcatgtg gcaccagagc
     1321 ccactgttcc tgcagcgagt ggccttcgat cacctgctgg cggtggcgct gggaattgcc
     1381 atggtggtgc tggtactgga agaagcgcgc gcccgcaccg aagaactcca cgacaaactg
     1441 aaccggctga gcttgttgat tgccgccagc acgcaaacgc ttaccgtccg ggaaatgctg
     1501 gaccgtgtgc tggtcaacgt cgtcggcagt ctgggggtgt cgcacggctt ggtgcggctg
     1561 ctgcaaggcg aggggaactc tgcgcaactg gcggtgcaag cagcgacggg attcgacgaa
     1621 agttatttgc agacacacca cgtaataccg gccaatgaac cgtgggcgca gcgtgttttg
     1681 aaggaagatt acacgtcggc caagatcggc gaagaagcgg acccgcgcga acggcagcgg
     1741 atgtcggcca ccggagtgcg cgaaatcgcc accattgtgc tgcgcggcaa ggaccgcccg
     1801 ctggggatcc tggctgttgg ctgcgcgcag gagatgaaat ttcagccgga cgaaatcgat
     1861 tacctgaaaa acatcggcaa cttgctggga ctcacgctcc aaagcgcgct gctgtttcag
     1921 cagttggaca acgtacagcg ccagtgggct tttacctttg attcaatcgg cgatccgatc
     1981 gtggtgcacg acggcgaagg ccgcatcctg cggctgaatc agcgcatggc ccaactgagc
     2041 ggccgcgaac tcaagtcctg gatcggccgg aatatcgccg agttattttc gcgtaagcag
     2101 gccgactaca cgcggtgccc ttattgcgag ggagcggcgg gagaaggcga cgatcccgac
     2161 ccgtggttgt ccggatattt cctgacctcc aattctccgt ttgtggattc caatggcaac
     2221 cagatgggcg tgatccacgt attgaaggac atttccgagc gcaagcgcgc cgaagaaaag
     2281 tatcgaacac tgatttccaa tgtacaggaa ggcgttttta tttcgacgcc gcaggggcgt
     2341 ttccttgatt tcaacgacgc ctttctgcgt atgactggat acacttcacg ggaagaattg
     2401 atgaatttgg atgttcaggc gctctatgcc agccccgccg accgcgaccg gttgcggaaa
     2461 ttgctggcgg actatggctc ggtggcggat tttgaatttg agattcagcg gaaagacggc
     2521 gaaacacgca ccgttctgga gtcttctaac gccgtcaaag attccacggg caacatcatt
     2581 gcgtatcaag gattcatgct ggacattacc gagcgcaagc gcgcggaaca tgagattcgc
     2641 cggcgcaacc gcgagctgct ggttctgaat tccatcgcca ccacgctgaa cgagtcgctg
     2701 gacctaagcg actccttgca caagacgctg aggcaaattt gcgaactgtt cagcctggaa
     2761 gcctcttcgc tgtatctttt cgattccggc aatgaaatgt tgcgtcgcgt ggccgcattt
     2821 ggccaccgtt ccgagtttgc gcgaaatttc ccgcccacac gcgtccaacc ggaactgctg
     2881 caacacattc gcgccgtgcg agccatgttc ctgtccattc aaggactgcc gttgccgcct
     2941 atcttcagag aactgcagcg ggccgagcag attgtggccg cgcatatcgt gatcctgtgg
     3001 tccaaagaca aagtcatcgg cggactggta gtgggcagcc gcgcgccgcg cgatttttcg
     3061 ccgggtgacg tgaacctgct catcgccgtg ggcagccaga tttccagcgc gattgagcgt
     3121 acgctgctct acgaccagac acgtcaggcc tatgacgatc tgcgtcgcac ccaggaacag
     3181 cttttgcata gcgagaagat ggcggccgtc ggccagctca tctccggcgt tgcgcacgag
     3241 ctaaacaatc cgctcaccgc gattctaggc tacagccaac tgctgacctc ctgcggacaa
     3301 gtcggccagc aggggctgga gtacagcgaa aaactgtaca agcaggcgca acgcacgcat
     3361 cgcatcgtgc aaaatctgtt gagcttctcg cggcagcaca agccggagcg cgttccggtc
     3421 cgtttaaatc agattctgga agatacgctg gcgctgcgcg actatgattt gcgcatgggg
     3481 aacattcgct tgcatctcga gctcgccccc gacttgcctt ccgccatggc cgatccgcac
     3541 cagttgcaac aggtcttctt gaacctcgtg aataacgcgg tcgacgccat gctcgagtgc
     3601 tccgcgagtg gagatttatg ggtacggacc gcactcgaga acaacaagtt cattttggaa
     3661 tttgtggaca gcggccccgg cgtgaaggat ggctcgcgcg tatttgaccc gttttacacc
     3721 accaagccgg tgggcaaggg aaccggtctg ggcctgagca tttgttatgg gatcatcacc
     3781 gaacatggcg gacagattcg cgtgcgcaac gtgccgccgc ggggagcctg cttcacgatc
     3841 gaactccccg gtccaagccg gagcagcggc acagaatctt ctgttttgga aacggtcgct
     3901 tccggccccg gctccacagc cttgaactga acgaaatcgc gaaatcttat tggtcggatt
     3961 gcaaggccag gtctgccgcc agtgcggccc gttcgccgag tgtcgccatc gcggtttcca
     4021 tccgaatggc cagcgaacta accatttggc ccaaagaggc gagctgaagc atggcgacgt
     4081 cgccgaacaa gaagtgcagc ttcgccagtt gaaagcgaac ccggaagagg atggaaaggc
     4141 gcaggcgctc atactcgtgg gagctggaaa cttccggcga aagcaaggct actacgcgcg
     4201 cgatgcgcag caactgatcg aagtctgagc gcagggattc caggtacagc aaggccacgt
     4261 ttcgccgttc gcggcg
//
LOCUS       DASTPM010000474         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_593460, whole genome shotgun sequence.
ACCESSION   DASTPM010000474 DASTPM010000000
VERSION     DASTPM010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593460"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /gene="coaE"
                     /locus_tag="VFR42_08400"
     CDS             complement(<1..572)
                     /gene="coaE"
                     /locus_tag="VFR42_08400"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU0369214.1"
                     /translation="MLRYGLTGGIASGKSTVAAMLREHGFAVLEADKISHGLIEPGGA
                     AYEEVIARFGREIVHADGRIDRGRLGAMVFHDPEELKQLNGLLHPRVEAELVRSLAEL
                     EISGSVAVAFVEAALIFEAGLDKGLDGVVVAWCLPEQQLARLMERGMSEAEARQRIGA
                     QMPVQEKLALAAEKIDCSGSIEETRSQVDAL"
     gene            complement(581..1543)
                     /locus_tag="VFR42_08405"
     CDS             complement(581..1543)
                     /locus_tag="VFR42_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEU0369215.1"
                     /translation="MTARILDGAKIRDEIFAELKQEVLQLAAQGVRPGLAAVLVGENP
                     ASQLYVKSKIAACEQLGLASWLHTPPANVTTEWLLQLVTDLNHDDNVDGILIQLPLPG
                     QVDSKRVLEAVSPAKDVDGFHPVSMGMLVSNRPGLVACTPAGCMEILRRNGIPIEGAN
                     AVVLGRSDIVGKPMALLLLHANATVTVCHSKSRDLPGIIGRADILVAAIGRAGFVQAD
                     WIKPNAAVIDVGTNKVTDATEAKRLLARFPARLEKFAAKGNVLVGDVHPDAVNTAGAL
                     TPVPGGVGPMTITMLMSNTVKAAKLRRSVAATNESKSQPAATAG"
     gene            1651..2268
                     /locus_tag="VFR42_08410"
     CDS             1651..2268
                     /locus_tag="VFR42_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEU0369216.1"
                     /translation="MTLFPSDGNRKQLENQARAEGRLPPGQSLTLKWPVLHYGSVPHF
                     DESKWDFKISGEVAAPAKLSWKEFLALPQTEVTSDFHCVTRWSRLDNRWTGVLFTEVL
                     KLVQPTPAAKHALVLAEEGYTSNIPLADLLHPDVLFANSHDGRPLSAEHGGPLRLIVP
                     HLYAWKSVKWVRGFVLLSEERLGFWERNGYHAYGDPWKEQRYSGT"
BASE COUNT          487 a          961 c          586 g          551 t
ORIGIN      
        1 agcgcatcca cctggctccg cgtctcttct atagaacccg agcaatcgat tttttctgcg
       61 gccaatgcca acttttcctg caccggcatc tgcgcgccga ttcgctggcg tgcttccgct
      121 tcgctcatcc cgcgctccat taggcgggcc agttgctgtt ccggcaggca ccaggccacc
      181 accacgccgt cgagcccctt gtccaaccct gcttcaaaaa tcaacgccgc ttccacaaac
      241 gccaccgcaa ccgagccgga tatttccagc tccgccaagc ttcgtaccaa ttccgcctct
      301 actcgcgggt gcagcaaccc gtttaactgc ttcaattctt ccggatcatg aaacaccatc
      361 gcgcccagtc gcccgcgatc gatccttccg tccgcgtgca caatctcgcg tccgaatcgc
      421 gcaatcactt cctcatacgc cgcgccgccc ggctcgatca gcccgtgcga aatcttgtcc
      481 gcttccagca ccgcaaaccc gtgctcgcgc aacatcgccg ccaccgtgct cttcccgctg
      541 gcgattcccc ccgtcaagcc gtagcgcagc atgtatcgat tcagccggcc gtcgcggccg
      601 gctgagattt gctttcgttt gtcgccgcaa cactgcggcg cagcttcgcc gccttcaccg
      661 tgttgctcat cagcatggtg atggtcatcg ggcccactcc acccggaacc ggcgtcagcg
      721 ctcccgcggt attcaccgca tccggatgca cgtcgcccac cagcacattt cctttcgccg
      781 caaatttctc cagccgcgcc ggaaaacgtg ccagcagccg tttcgcttct gtcgcgtccg
      841 tcactttgtt ggtgccgacg tcaatcaccg ccgcgttagg cttaatccag tccgcctgca
      901 caaatcctgc ccgcccgatc gccgcgacca aaatatctgc ccgtccgata attcccggca
      961 aatcccgcga tttcgaatgg cacaccgtca ccgtggcatt cgcgtgcagc agcaacaatg
     1021 ccattggctt gcccacaatg tcgcttcgcc ccagcaccac cgcattcgcg ccctcgatgg
     1081 gaattccgtt gcgccgcaaa atttccatgc aacccgccgg cgtgcatgcc accaggcccg
     1141 gccggttgct taccagcatc cccatgctga ccgggtgaaa cccatccaca tcttttgccg
     1201 gcgaaaccgc ttccaatacg cgtttggaat ccacctgccc cggcagcggc agctgaatca
     1261 gaatcccatc tacgttgtca tcatgattca aatcggtcac cagctgcagc agccattcgg
     1321 tggtcacgtt agcgggaggc gtgtgcagcc agctcgccag tcctaactgc tcgcacgccg
     1381 caatcttgct cttcacataa agctgcgaag ccgggttttc tccaaccagc accgccgcca
     1441 gtcccggccg cactccctgt gccgccagct gtaacacttc ctgtttcaac tccgcgaaaa
     1501 tttcatcgcg tatcttcgct ccatccaaaa tccgtgcggt catgcaaaaa gcatagcaca
     1561 gcgaacctcg ccgccacttt ctgcgaacga tcgccaatgc aacggaaact ttccgctccg
     1621 cttataatcg aatgtaccgg acgaactcct atgactctct ttccctccga cggcaaccgc
     1681 aagcaactcg agaatcaggc gcgcgccgaa ggccgcctac ctcccggcca atccctcacg
     1741 ttgaaatggc ctgttctcca ctacggttct gtcccgcatt tcgacgaatc caagtgggac
     1801 ttcaagatct ctggcgaagt cgccgctccg gccaaacttt cctggaagga atttctcgcg
     1861 ctcccgcaaa ccgaagttac ttctgacttt cactgcgtca cgcgctggag ccgcctcgac
     1921 aatcgctgga ccggcgtcct cttcaccgaa gtcctcaagc tcgtgcagcc cactcccgcc
     1981 gccaaacacg ctcttgtcct cgccgaggaa ggatacactt ccaacattcc gctcgccgat
     2041 ctcctccatc ccgacgttct tttcgcaaac tcccacgacg gccgaccgct cagcgccgaa
     2101 catggcggcc cgctccgcct catcgttccg catctttacg cctggaagtc cgtcaagtgg
     2161 gtccgcggct ttgttcttct cagcgaagag cgtctaggtt tctgggaacg caacggctac
     2221 cacgcctatg gcgacccgtg gaaagagcag cgctactctg gaacgtagat atgacctttc
     2281 gacactaccc tgcgaccttt gcgtctatgc gtttcgtttt ctgaactctt cgcctcttgt
     2341 gcgaagatgt tccgcgctga actgttcggt ggagccgcca tgcgtcgcct gttcatcttc
     2401 ttctcaacat tgctcttgtc ttcgaccgcc ctctccgcgc aacaaacccc cctggcaacc
     2461 tccagccaac cgactgacca tcaacctgtg gccgagcgca gttcccggcg cgccggctaa
     2521 ctcccctccc gaaaccgata tcaccaccag caccgatacc cgcatcgccg gcaggcgtct
     2581 tgtcc
//
LOCUS       DASTPM010000475         6647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_160765, whole genome shotgun sequence.
ACCESSION   DASTPM010000475 DASTPM010000000
VERSION     DASTPM010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160765"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VFR42_08415"
     CDS             <1..246
                     /locus_tag="VFR42_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369217.1"
                     /translation="GAVVFGCFTLRLRLYGGTGCQKRGSTAKATRQGPTQSKSSGTSA
                     SKYEGFGFFWLRNLLAWAPNLFDGRLVRALESLGLSG"
     gene            322..1983
                     /locus_tag="VFR42_08420"
     CDS             322..1983
                     /locus_tag="VFR42_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU0369218.1"
                     /translation="MAQQAQQAPDPKDPPAPQKLTKEQKKKMGRALKELDAQYKQWLQ
                     EDVVYIISPEERTAFLQLSTSEEREQFIEQFWLRRSSNPDLPENDFKEEHYRRIAYAN
                     EHFASGIPGWRTDRGRIYIIWGPADEVESHPTGGTYDRPMEEGGGSTTTYPWETWRYR
                     YLEGLGNNVILEFVDPSGSGEYHLTMDPSEKDALLHVPGAGLSLMESMGLASKADRFT
                     RSDGTNLPTTMGGQPASMNEFTRLEQYAKIQQAPPVKFKDLEALVTSRIVRDQVHFAW
                     RTDFLKVTSDTVLVPVTVQVPNGQLSFQAKDGVHTATMNVFGRVSTLTGRVVQTFEEA
                     ISRDFPDSLFQQSVKLQSIYQKAVPLRPGLYRLDLVIKDVQSGNVGVVNTRLQVPRYE
                     DEKLEASSLILADQIERVPAKQIGAGQFVIGSSKVRPRLEGDFTAQDKLVIYMQVYNL
                     KPDEQTHKSNATFLYTVKKAGDKADQPAIMQFKESSVDMKQTGDQITIERLLPLATLA
                     PGKYKLEVSATDALSNQTISRSAEFTVKPPAAPIKTSAANTTPGR"
     gene            2054..3745
                     /locus_tag="VFR42_08425"
     CDS             2054..3745
                     /locus_tag="VFR42_08425"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0369219.1"
                     /translation="MSFLTSAADAQAKLVAGSLTGVVRDLAGTPQMGASVEVLAETAG
                     INSTSEFLTNTQGIFRSDKLSPGLYTIRVTLAGFLPTLQQHVRINANLTTMVRIEMES
                     MFATLEQLRKQPNSAKAEAEDWKWVLRSASNTRPVLQWDDYGAVLSAGVDRNAGAPKM
                     LMQFTDGARRPGSVSNVAAAPGTAFAYDQKLGEGKLLVAGQMSYDEAPAGGVATVWLP
                     TGTIGAGPHTALVLREAALGNTGMTFRGMRLDQGGAVTLGDRMLVRYGGEYLLVGLGR
                     SVSSLRPRISLETKLSDTWRANVVFASQPGAPNSLDSNALDASGDELQSSLVRALDEL
                     DAFPALMWRDGRPVLQGGWHEEISAERRVGERGKLQVAAFHDDAHHLAVFGRGSDLPE
                     QEYFQDIFSNAFAYDGGMASSWGGRIAYREKFGDDTELTAVYAMGGALAPGASTEAVL
                     RDALRMATRQSLGLNVSTKVPRAGGRVTVGYKWISGPTITRVDSYGESLYQMDPYLHV
                     SLRQQLPKFGPGRWEAIADCDNLLAQGYVTMNTREGQVVLVPAFRTFRGGLSLQF"
     gene            3927..>6647
                     /locus_tag="VFR42_08430"
     CDS             3927..>6647
                     /locus_tag="VFR42_08430"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0369220.1"
                     /translation="MKQSFRVSLGAVVLALATLAAVIFAILNFDQRARFESPDDGVAW
                     RDTDHGIEAWKVTIGGPAWQAGIRQGDRLLEIAGQPVPGAVQVTKRVWRAGLWSQLRY
                     KVERGGESFETPVVISPAEKPLTAENYLRVVGLIYLFIGIFIFIRRWNAPRAVHFYVF
                     CLVSFILYSFHYSGKLDSFDYEIYWARVVAGLLAPALLLHFALVFPERAQSSLKEFAK
                     LSFVYGPPLLLLLAHLNVAMQALGFMPWLGSRVLLDQIELGYLATCFVLAGLVFYHSY
                     RHAPSGVLRQQLKWLAGGTLAGSLPFALFYILPYTLNPLQKPWMVFSSLSLVLIPLCF
                     GYAIIRYRLMDVDIIFKRGLAHTAATAAVAAVFFSLVGLSGDIFHTNTSDRLVVVLAI
                     TIASFLFQPVREWIQRRLDHFFYRDRLDYRRTLIEFGRTLTNEVRLDPMLSSVMDRIS
                     QTLLVDRLAIFVEDDQQPGKMKLARSMGVRVHNPLDLSFLDTARPEMARGALFFESPR
                     SVQGASEPVRGTVEQLDLNYYVPCRIREHCVAVLGLGKTVDGDFLSSDDVELVQTIAG
                     YVAVALDNAQLYSSLEQKALEIARLKDFSENIVESLNVGVLAVDLSGIVESWNTRMEQ
                     LFGVLRQDAVGTKLDALLPAELATEIAARRDQEQITGIYKQRLQHQGKTLTLNVSITP
                     LVSKSGEHIGRLLLFDDVTQRERMEEQMSQTEKLTSLGLLAAGVAHEVNTPLAVISNY
                     IQMLAKQMPESDPRHSIIEKIVKQTFRASEIVNNLLNFSRTGAAETTNVDVNRVVEDT
                     LALVSHPLKASQIQVVKNLSDGLPAVRGSANKLQQVFLNLFLNARDAMPSGGILEVRT
                     NAHNGSVEIEVVDTGAGIPREHIHRIFDPFFTTKASGRGKGLGLSVSYGII"
BASE COUNT         1369 a         1791 c         2142 g         1345 t
ORIGIN      
        1 ggtgcggtgg ttttcggctg tttcactttg cgactgcgcc tgtacggcgg tacaggttgc
       61 cagaaacgcg gctccacggc taaggcaacc cggcaaggac caacccaatc taagtcatcg
      121 gggacaagtg cctcaaaata cgagggattt ggttttttct ggctgcgaaa tcttctggca
      181 tgggctccga acttgtttga cgggcggctt gtccgggcgt tagaatcttt aggtttaagc
      241 ggttgaggtg taccgatggc cagggtagtc cgaaccctat ttgctacagt tttctctata
      301 ttgatttccc tcccgccatc aatggcgcag caggcgcagc aagcccctga tcccaaggat
      361 cctcccgctc cacaaaagct gaccaaagaa caaaagaaaa agatgggccg ggccctgaaa
      421 gagctggatg cccagtacaa gcagtggctg caagaagacg tcgtgtacat catctcgccg
      481 gaagagcgta ccgcgttttt gcagttgagc accagcgaag agcgcgagca gttcattgag
      541 cagttctggc tgcggcgcag cagcaatccg gacttgccgg aaaacgattt taaagaagag
      601 cactaccgcc ggattgccta cgccaacgaa cactttgctt cgggcattcc gggttggcga
      661 accgatcgcg ggcgcattta catcatttgg ggaccggcgg acgaagtcga gtcgcatcct
      721 acgggcggaa cgtacgaccg gccgatggaa gaaggcggcg gttcgactac aacttatccc
      781 tgggaaactt ggcgctaccg ctatctggaa gggcttggca acaatgtcat tttggaattt
      841 gtggatccat ctggttccgg tgaatatcac ctgacgatgg acccatcgga aaaagacgca
      901 ttgctgcacg tgccgggcgc gggtctcagc ttgatggaat cgatgggcct ggcgtcgaag
      961 gcagaccggt ttacccgttc cgatggcacc aatctgccga cgactatggg cggccagccg
     1021 gccagcatga acgaattcac gcggctcgag cagtacgcga agattcagca ggcgccgcca
     1081 gtcaagttca aggatttgga agctttggtg acctcacgta tcgtgcgcga ccaggtacac
     1141 ttcgcgtggc gcacggattt tctgaaggta accagcgata cggtgctggt gccggtgacc
     1201 gtacaggtgc cgaacggcca gctttcgttc caggcaaagg acggggtaca cacggcaacg
     1261 atgaacgtct tcgggcgtgt gagcacgctg actggccgcg tggtgcaaac atttgaagaa
     1321 gccatcagcc gggactttcc cgattcgctg ttccagcaat ccgtgaagct gcagtcgatc
     1381 tatcagaagg cggtgccgct gaggcccgga ctctaccggc tggatctggt gatcaaagac
     1441 gtgcagagcg gcaacgtggg cgtggtgaat acgcgcttgc aggtgccccg atacgaagat
     1501 gaaaagctgg aagccagctc gctgatcttg gctgaccaga tcgagcgcgt gccggcgaaa
     1561 cagattggag ccgggcagtt tgtgatcggc tcatccaaag tgcggccgcg actggaaggc
     1621 gatttcacgg cgcaggacaa actggtaatc tacatgcagg tgtacaacct gaagccggat
     1681 gagcagaccc acaagtccaa cgcgacgttc ttgtacactg tcaagaaggc cggcgacaag
     1741 gccgaccagc cggcgatcat gcaattcaag gaaagcagcg tagacatgaa gcaaacgggc
     1801 gatcagatta cgattgagcg cctgctgccg ctggccactc tggcgcctgg caagtacaaa
     1861 ctggaagtga gcgcgaccga tgcgctttcc aaccaaacga tttcccgaag cgcggagttt
     1921 acggttaagc cgccggcggc gcctatcaag acgtcggccg cgaacaccac accggggagg
     1981 taacgtggag caacagggac ggctccggag accattccgg atttggggga ccctgtttat
     2041 ggccgcggcg tgcttgtcat tcttgacgag cgcggcggac gcgcaggcga agctggtagc
     2101 cggcagcctg acgggagtgg tgcgcgacct tgcgggtacg ccgcagatgg gcgcaagtgt
     2161 ggaagtactg gccgaaacgg ccggcatcaa tagcacgagc gaatttctca ccaatacaca
     2221 aggcatcttc cgtagcgaca aactgtcgcc gggtctgtac acgattcgcg tgaccctcgc
     2281 gggatttctg cctacgctcc agcagcatgt gcgcatcaat gcgaacctga ccacgatggt
     2341 gcgcattgag atggagtcga tgtttgccac cctggagcaa ctgcgcaaac agccaaactc
     2401 tgcaaaagcg gaagcggagg actggaagtg ggtgttgcgt tcggcttcga atacgcggcc
     2461 ggtgctgcaa tgggatgact acggcgcggt gcttagcgcg ggcgtggacc gcaacgcggg
     2521 cgcgccgaaa atgctgatgc agtttacaga cggcgcgcgg cggccgggat ccgtttccaa
     2581 cgtagcggcg gcaccgggca cggcttttgc ctacgaccag aagctgggtg aaggaaaatt
     2641 gctggttgcc gggcagatga gttacgacga agcgccagcc ggcggcgtgg caacggtatg
     2701 gctgccgacc ggaaccattg gcgcgggtcc ccatacggct ttggttctgc gcgaagcggc
     2761 gttaggcaat accggcatga cgttccgggg catgcggttg gaccagggtg gcgcggtaac
     2821 tctcggcgac cggatgctgg tgcgctacgg cggagagtac ctgctggtcg gattgggccg
     2881 gtcggtatcc tcgctgcgtc cgcgaatcag cctggaaacg aaactaagcg acacgtggcg
     2941 cgcgaacgta gtgttcgctt cgcagccggg cgcgccgaat tcgctggact cgaacgcgct
     3001 cgatgcgagc ggcgacgaat tgcaatcttc actggtgcgg gcgctggatg agctggatgc
     3061 gtttcccgcg ctgatgtggc gcgacgggcg gcccgttctg caaggcggat ggcacgaaga
     3121 gatttccgcc gagcgccgcg tgggcgagcg aggcaagctg caggtcgccg cgtttcacga
     3181 cgacgcgcac cacctggcgg tgttcggccg tggcagcgat ttgccggaac aggaatactt
     3241 tcaggatatt ttttccaacg cgtttgctta cgacggtgga atggccagca gctggggcgg
     3301 ccgcatcgct taccgcgaaa agtttggcga cgacacggaa ctgactgcgg tctacgccat
     3361 gggcggagcg ttggcgcctg gggcctcgac cgaagcggtg ttgcgtgatg cgctgcgaat
     3421 ggcgacgcgg cagagcctgg gcctgaacgt ttccacaaaa gtgcctcgcg ccggtggacg
     3481 cgtgaccgtc ggatataaat ggatcagcgg acccaccatt acgcgcgtgg acagctacgg
     3541 cgagtcgctc tatcagatgg atccctacct gcatgtttcg ctgcgccagc aattgcccaa
     3601 gttcggcccc ggccgctggg aagcgatcgc ggattgcgac aacttgctgg cccaaggcta
     3661 tgtgacgatg aacacgcgcg agggccaggt ggtactggtt ccggcgtttc ggacgtttcg
     3721 gggtggcttg agcttgcagt tttaagggtt tggaaagctg taggcaccct tccagaaagt
     3781 ggatttccgc tgccagtttc ctcgagggtt cttctaaggt acagaaaata ggtcgattag
     3841 agccaacaca agcctcccaa gagtggaaga gcagttgcac gagactttcg caagtgtgtt
     3901 ggccataagg tgcgtggatc ctgtccatga agcaaagttt tcgcgtcagc ctcggagcgg
     3961 tggttttagc gctagcgacg ctggctgcgg taatctttgc cattctgaat tttgaccaac
     4021 gggcgcggtt tgaatcgccg gatgacggcg tggcatggcg cgacacggac cacggaattg
     4081 aagcctggaa ggtaacgatc ggggggccgg cgtggcaggc gggcatccgc cagggcgacc
     4141 ggctgctgga gattgccggg caacccgtgc cgggcgcagt gcaggtgaca aagcgagtgt
     4201 ggcgcgccgg gttgtggagc cagctgcgct acaaagtgga gcgtggcggc gagtcgttcg
     4261 aaactccggt agtgatttct cccgccgaga agcccttaac cgccgagaac tacctgcgcg
     4321 ttgtcggcct gatttatctc ttcatcggaa ttttcatttt catccggcgg tggaatgcgc
     4381 cgcgcgcggt gcacttctac gttttctgcc tggtgtcttt cattctgtac agctttcact
     4441 acagcggaaa actggacagc ttcgactacg aaatttattg ggcacgtgtc gtggccgggc
     4501 tgctggcgcc ggcgctgctg ttgcacttcg cgctggtgtt tccggagcgc gcgcaaagct
     4561 cgttgaaaga atttgcgaag ctgagttttg tctacgggcc gccactgttg ttgctgctgg
     4621 cccatctgaa cgtggccatg caagcgttgg gcttcatgcc ctggctcggc tcgcgggtgc
     4681 tgctcgatca aattgaactg gggtacctgg cgacctgctt tgttctggcc ggactggtct
     4741 tttaccacag ttaccgtcat gcgccttcgg gagtgctgcg gcaacaactg aagtggctgg
     4801 ccggcggcac gctggcgggc agtttgccgt tcgccctgtt ctacattttg ccttacacac
     4861 tgaacccttt gcagaagccg tggatggtct tttcgtcgct gagcctggtg ctgattcccc
     4921 tgtgcttcgg gtacgccatc atccgctacc gcttgatgga cgtggacatc atcttcaagc
     4981 gcggcctggc gcacactgcg gcgacggcgg cggtggcggc ggtctttttc tcgttggttg
     5041 gactgagcgg agatattttt cacaccaaca ccagcgaccg cttggtggtg gtgctcgcca
     5101 tcaccatcgc ttcgtttttg tttcagccgg tgcgcgaatg gatccagcga cggctggatc
     5161 atttcttcta tcgcgaccgg ttggattacc ggcggacgct gatcgagttt gggcgaaccc
     5221 tgaccaacga agtgcggctc gaccccatgc tgagttcggt gatggaccgg atttcgcaga
     5281 cgctgctggt cgatcggctg gcgattttcg tggaagacga tcagcagcca gggaaaatga
     5341 agctggcgcg ctcgatggga gtacgcgttc acaatcctct tgatttgagt tttctggata
     5401 cagcgcggcc ggagatggcg cgcggagcac tgttttttga atcgccgcgg agcgtgcagg
     5461 gtgcgagcga gccggtacgc ggaactgtcg agcaactgga tctgaactat tacgtgccgt
     5521 gccggattcg cgagcattgt gtggcggtgc tgggacttgg caaaaccgtc gacggcgatt
     5581 tcctctccag cgacgacgtg gaattggtgc agaccattgc gggatatgta gccgtcgctc
     5641 tggataacgc acagctctac agctcgctgg agcaaaaggc gctcgagatc gcgcgcctga
     5701 aggacttcag cgaaaacatt gtggagtcgc tgaacgtcgg cgtgctggcg gtggatctaa
     5761 gcgggatcgt ggaaagctgg aacacgcgga tggagcagct ttttggggta ctgcggcaag
     5821 acgcggtggg aacgaagctg gacgcgctgc tgccggctga gctagccacg gaaattgccg
     5881 cgcgccgcga ccaagaacag attaccggca tctataaaca gagactgcaa caccaaggaa
     5941 agacgctgac actcaacgta tccattacgc cgctggtgag caaatccggc gaacacatcg
     6001 gacgcctgct gctgttcgat gatgtaacgc aacgcgagcg catggaagaa cagatgtcgc
     6061 aaacggaaaa gctgacatcg ctgggattgt tggcggctgg cgtggcgcat gaagtgaaca
     6121 ctccgttggc tgtgatctcg aattacatcc agatgctggc caagcagatg ccggaaagcg
     6181 atccgcggca ttcgatcatc gagaagatcg tgaagcagac cttccgcgcc agcgaaatcg
     6241 tcaacaacct gctgaatttc tcgcgcaccg gcgcggcgga aaccaccaac gtagacgtaa
     6301 accgcgtggt ggaagacacc ctggcgctgg tgtcgcatcc gctgaaggct tcgcagatcc
     6361 aggtagtgaa gaatttgtcg gacggattgc cggcagtgcg cggctcggcg aacaagctgc
     6421 agcaggtttt cctgaacctg ttcttgaacg cgcgcgatgc catgccctcc ggcggaattc
     6481 tggaagtgcg caccaacgcg cacaacggca gcgtggaaat cgaagtggtg gacacgggag
     6541 cgggaattcc gcgtgagcat attcaccgga ttttcgatcc cttctttaca accaaggcca
     6601 gtgggcgagg caagggtctt gggctttccg tgagctacgg aatcatc
//
LOCUS       DASTPM010000476         6752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1227748, whole genome shotgun sequence.
ACCESSION   DASTPM010000476 DASTPM010000000
VERSION     DASTPM010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227748"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1965)
                     /locus_tag="VFR42_08435"
     CDS             complement(226..1965)
                     /locus_tag="VFR42_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0369221.1"
                     /translation="MKFAKLAKLLLATAVLWVIPGDAQSPSPAPAPKARPQEAAAPVA
                     RPKLVVLLVVDQMRGDYVDNFQGQWKGGLKRLVKEGAWFREAAYPYAATETCVGHSTI
                     STGALPTTHGMVANAWWERDSQKMVTCTFDPSVSNTAYGGGAAIAGGDSAWRMRVPAY
                     AEELRFQSGGATRIVSFSLKARAAITMAGHKGDAVTWFENGSWTTSSAYGTMPFVENF
                     AKTNPVKADYGKTWALSLPESTYFYDEKATGAVAPGGWDLTFPHPLRGNPDSSEPDSA
                     FYLQWATSPYADTYLTKMAENAVDQLGLGKSGGTDFLGVSYSSVDYVGHAFGPHSWEI
                     QDVLVRLDKDLAELFAHLDAKVGRGNYVVALSADHGVVPAPEDMQKTGADAGVLHLPE
                     IQAKIEKALEPFNYTKPAVARVASSDVYFSAGIYERLKTDAPAMRAVLDGIRSVPGVA
                     EVYRAEELQDRPATQNPIQRAEAASYFAGRSGDLMIVPKPYWLVSGGSAGKPRDYGTG
                     HGTPYLYDQRVPVLLMGWGIEHGEFFGRVTPADIAPTLAALTGVTLSSRDGRVLAEAL
                     RGSAKAKAPVAKP"
     gene            2060..2608
                     /gene="pyrR"
                     /locus_tag="VFR42_08440"
     CDS             2060..2608
                     /gene="pyrR"
                     /locus_tag="VFR42_08440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU0369222.1"
                     /translation="MRIVEKGQLMSAAEIDRTLQRVAHEIVEKSGGTKHLALIGIRRR
                     GVPLAQRIAQAMRGIGGVEVPVGTLDITLYRDDLSKVAPQAVLQSSEIPFGVDGMDLV
                     LVDDVLYTGRTIRAAMNGLFDLGRPRRVSLCVLIDRGHREMPIEAQYIGRVVQTSDTE
                     IIEVRLREIDNEERVVLVDRVE"
     gene            2657..3697
                     /locus_tag="VFR42_08445"
     CDS             2657..3697
                     /locus_tag="VFR42_08445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU0369223.1"
                     /translation="MSYRFRDLLALEPLSAAEISFLLDQSKPFQEIQRHPLKKLATLR
                     GKTVALAFFENSTRTRISFNTAASRLGADTMNLQAEASSLKKGETLLDTAFNLNAMKP
                     DCLVMRHFSSGAADYVSRHLDIPVVNAGDGTHEHPSQGLLDALTIRDRKGTIENLNVT
                     ILGDLSHSRVARSDIHLLGKFGCRFTLCGPAMWVPRELEKIAPGTPIRSVYKIEQALE
                     DADVIIMLRVQTERQHEASISANDYIHGYQLNAERLKRAKPDALVLHPGPMNRGIEIA
                     PEVADGPQSCVLDQVTNGVAVRMAILYLLVVGEQGTHPAAPAPVEHNRRATDRSSPSS
                     ASPEPVPQKRNS"
     gene            3700..4974
                     /locus_tag="VFR42_08450"
     CDS             3700..4974
                     /locus_tag="VFR42_08450"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0369224.1"
                     /translation="MLLIKNGRVLDPASKTDAALDVLLDGEVIKEVGAPGKFAPANNT
                     EVFDASGMIVAPGFIDLHVHLREPGQESSETIETGTKSAARGGFTAVCCMPNTKPVHD
                     NASITRFIVDKARTVGATRVWPIGAASVGSQGEAIAEIAAMKEAGIVAVSDDGKPIAT
                     ARLTRQVMDYCRSLDLPVIEHSEDVSLAAGAVMREGVTSTRLGLRGMPAAAESVCVSR
                     DVQLAELTGARLHIAHLSAKASLDQVRWAKDRGLRVTCEVTPHHFTLTDEDVTYDSRF
                     KMNPPLASREDREALIAGVADGAVDVIATDHAPHEPALKDVEFDRAPFGILGFETAIP
                     LALEQLVATNKIPLMRMVELFTTGPARVLGIERKLAAGQPADLTIFMTSQLWTYNVND
                     SPSKSRNSPFDGRTFSGAPMATIVAGKLAFRR"
     gene            5100..6374
                     /gene="serS"
                     /locus_tag="VFR42_08455"
     CDS             5100..6374
                     /gene="serS"
                     /locus_tag="VFR42_08455"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU0369225.1"
                     /translation="MHDLSYFRDHLDVFADMAKRRNITLDLDTFRAIDKERREFITAN
                     EQRKAQRNKASDEIARRKKDKLNADALIAEMKQVSEQIKQSDEKISELETKQRDLMLV
                     VPNLPHSSVPTGTGSEDNVEVRRWGTPPKLDFQPKPHWEVGERAGILDLAAATKVTGA
                     RFAIYKGWGARLERALANFFLDVHTREHGYTEILPPFLVNTASLMGAGQLPKFAADLF
                     KVENSDFWLTPTSEVELHNLHRDETLPEDKLPINVTAWTACFRSEAGAAGKDTRGILR
                     QHQFQKVELFKFTHPEKSYEEHEKLVANAETILQKLGLHYRSMLLCTGDMGFASAKTY
                     DIEVWLPSSNEFREISSCSNCEAFQARRAGIRFKPKAGGKSEFVHTLNGSGLAVGRTW
                     IAVVENYQQADGSILIPEALRPYMGIERIPSA"
     gene            6418..>6752
                     /locus_tag="VFR42_08460"
     CDS             6418..>6752
                     /locus_tag="VFR42_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369226.1"
                     /translation="MEYGKFAFRKGLVLALFLIGVLVLGGQRWFPGVYRLSFFLFLAG
                     SALAAYLRARERLSERSMSEQSIRAPGDWSANSERLVRDPVCGLYLAKGSALPLQHEL
                     ETLPFCSAVA"
BASE COUNT         1367 a         2125 c         1913 g         1347 t
ORIGIN      
        1 tgggcgaatt ccaggccata accgaacgtg ccgctggcag caatgatggg attgcggagg
       61 tgaagcgggc cgatggccac gctcaggtca acatcagccg cggaacggcc gcggttggaa
      121 gctggtgggg aactcactgc gtgaggagga taacatgaag ccttatcggc ccgacaattt
      181 catcggtggg cttacgtggg ttagagggac gctaaggttg aagtctcagg gctttgcgac
      241 aggagctttc gccttggctg atccgcgcaa ggcttccgcc aacacgcggc catcacgcga
      301 agaaagcgtc accccggtga gcgccgcgag ggtgggcgcg atatctgccg gtgtgactcg
      361 tccgaaaaat tcgccgtgct cgatgcccca gcccatcagc aggacgggca cacgttgatc
      421 gtaaagatag ggcgtgccat gtccagtgcc ataatcgcgc ggcttgcccg ctgaacctcc
      481 cgagacgagc cagtacggct tcggcacgat catcagatca ccgctgcgcc ccgcgaaata
      541 gctcgcggct tccgcccttt gaatagggtt ctgcgtggcc gggcggtcct gcaactcttc
      601 cgcgcgatac acttcggcaa ctcccgggac agagcgaatc ccgtccaata ccgctcgcat
      661 ggccggagca tccgtcttca ggcgttcgta gattcctgcc gagaagtaca cgtcgctgct
      721 ggctacgcga gccaccgccg gtttggtgta gttgaatggc tcgagcgcct tctcgatttt
      781 tgcctggatt tctggaaggt gtaaaacgcc ggcgtcggcg ccggttttct gcatgtcttc
      841 cggagcaggc acgacgccat gatccgcgct gagggccacg acgtagtttc cgcggcccac
      901 tttcgcatcc aaatgcgcga acaactctgc caagtctttg tcgagccgga cgagcacgtc
      961 ttgtatttcc cagctgtgcg gaccgaacgc gtggcccaca taatcgaccg acgagtagct
     1021 cactccgaga aaatccgtcc cgccactttt tccgaggccg agctggtcga cggcgttttc
     1081 cgccattttc gtcaggtatg tatccgcgta cgggctggtg gcccattgca ggtaaaacgc
     1141 ggagtcaggt tcactgctgt caggatttcc tcgcaaggga tgaggaaacg tcagatccca
     1201 gcctcctggg gccaccgcgc cggttgcctt ctcatcatag aaataagtgc tctcgggaag
     1261 tgacagggcc catgtcttgc cgtaatccgc cttcaccggg tttgttttgg cgaagttttc
     1321 gacaaatggc atggtcccgt aggccgacga agtcgtccat gaaccgttct caaaccaggt
     1381 gactgcgtct cctttgtggc ctgccatggt gattgccgcg cgcgccttca aggagaagct
     1441 cacgatgcgt gtcgcgccgc cgctttgaaa acgcaattcc tccgcatagg cgggcacccg
     1501 cattcgccac gcggagtcgc cgccggcaat cgcggcgccg ccaccatagg cggtgttgct
     1561 gacgctgggg tcgaaagtgc acgtcaccat tttttgtgag tcgcgctccc accaggcgtt
     1621 ggcaaccatg ccgtgcgtcg tgggaagagc gccagtggaa atggtggagt gccctacgca
     1681 cgtttctgtg gcagcgtagg gataggccgc ctcgcgaaac cacgcgcctt ccttgaccag
     1741 gcgcttcagg ccgcccttcc actgcccctg gaaattatcc acgtagtcgc cgcgcatctg
     1801 gtcaacaacg agcagcacca ccagcttggg gcgcgcgact ggagcggctg cctcttgcgg
     1861 gcgggctttg ggggccggcg ctgggcttgg actctgcgca tcgcccggaa tcacccacag
     1921 caccgcggtc gccagcaaca gttttgcaag tttcgcgaac ttcatgggcc ctcgtttcgc
     1981 ctgggcgagc agtctagcac gccagagaaa ttctccgatt ggtcgctggc atcggcgctt
     2041 atgctagaat tccgcgcaca tgcgaatcgt tgaaaaaggg caattgatgt cggcggcgga
     2101 aatcgaccgc acgctgcagc gcgttgccca tgagatcgtc gaaaaaagtg gcggaacgaa
     2161 acacctcgca ctgattggca ttcgccggcg tggagtgccg ctggcgcagc gcatcgctca
     2221 ggccatgcga ggcattggcg gcgtcgaggt tccggtaggc accctggaca tcacgctcta
     2281 tcgcgacgac ctctccaagg ttgcgccaca ggccgtcttg caatcctctg aaattccctt
     2341 tggcgtggac ggcatggatc tggtgctcgt cgacgacgtt ctctacaccg gccgtaccat
     2401 tcgcgccgcc atgaacggac tatttgacct tggccggccg cgccgcgttt cattgtgtgt
     2461 gctgatcgac cgcggccacc gcgaaatgcc aatcgaagcg caatacattg gccgcgtagt
     2521 acagaccagc gacaccgaaa ttatcgaagt tcgcctgcgc gaaattgaca atgaagagcg
     2581 cgtagtgctg gtggaccggg tagaataggg atgcgaattc tccgcgaatc gaccgacggt
     2641 gttactgtga gaggttgtga gctaccggtt tcgtgatctc ctggctctgg agccgctttc
     2701 cgcggcggaa atttcttttc tcctcgatca aagcaagccc tttcaggaaa ttcagcgaca
     2761 cccgctaaaa aaactggcca cgctgcgcgg caagactgtc gcactcgcgt tctttgaaaa
     2821 ctccacacgc acgcgcatca gtttcaacac cgccgcttcc cgccttggtg cggataccat
     2881 gaacctgcag gccgaagcct ccagcttgaa aaagggcgaa acgcttctgg acaccgcgtt
     2941 caatctcaat gcgatgaagc ccgactgcct ggtgatgcgc cacttttcct ccggtgcggc
     3001 cgactacgtc tcgcgccact tggacatccc cgtggtcaat gccggggacg gcacccacga
     3061 acacccctct cagggcttgc tcgacgcgct caccattcgc gaccgcaagg gcaccatcga
     3121 aaatctcaac gtgactattc tcggcgatct ttcccattcc cgtgtcgccc gctctgatat
     3181 tcacttgctc ggaaaatttg gctgccgctt cacgctctgc ggccccgcca tgtgggttcc
     3241 gcgcgaactg gaaaagatcg cccccggcac tccgattcgc agcgtttaca aaatcgagca
     3301 agctctggaa gacgccgacg tcatcatcat gttgcgcgtg caaaccgaac ggcagcacga
     3361 agcctctatc tccgccaatg actacattca cggctaccag ttgaatgccg agcgcctcaa
     3421 gcgcgccaaa cccgatgcgc tcgttctgca tcccggtccg atgaatcgcg gcatcgaaat
     3481 agcgcccgag gttgccgatg gcccgcaatc ctgtgtgctc gaccaggtca ccaacggcgt
     3541 ggccgtgcgc atggccattc tctatctact ggtggtcggc gagcagggca cgcatcccgc
     3601 ggctcccgct ccggtcgaac acaaccgccg cgcgactgat cgctcatcac caagcagcgc
     3661 gtcgccggaa cccgttccac agaagaggaa ctcttaagca tgttgctgat caaaaacggc
     3721 cgtgtcctcg atcctgcctc gaaaaccgat gcggcgctag acgtactcct cgacggcgaa
     3781 gtcatcaagg aagtcggggc gccgggaaag ttcgcgcccg caaacaacac cgaggttttc
     3841 gacgcgtccg ggatgatcgt cgcgcccggc ttcattgatc ttcacgttca tttgcgcgag
     3901 cccggccagg aatcttccga aactatcgaa acgggcacca agtctgccgc gcgcggcggc
     3961 ttcaccgcgg tctgctgcat gcccaacacc aagccggtgc acgacaacgc ctccatcaca
     4021 cgcttcattg tggacaaggc gcgcaccgtt ggcgccacgc gcgtctggcc catcggcgcc
     4081 gccagcgtcg gcagtcaggg cgaagccatc gctgaaattg ccgccatgaa agaagcgggc
     4141 atcgtcgccg tttccgatga cggcaagccc atcgccaccg cacgcctcac tcgccaggtg
     4201 atggactatt gccgctcgct cgatctcccg gtcatcgaac attccgaaga cgtttcgctc
     4261 gcggccggcg cagtgatgcg cgaaggcgtc acctccacgc gcctcggctt gcgcggcatg
     4321 cccgccgccg ctgaatcggt ctgcgtttcc cgcgatgtac agctcgccga actaactggc
     4381 gcgcgcctgc acatcgcgca tctctccgcc aaggcatcgc ttgatcaggt tcgctgggcc
     4441 aaggatcgtg gtctgcgcgt cacctgcgaa gtcactccac atcacttcac gctcaccgac
     4501 gaagacgtga cctacgattc gcgcttcaag atgaacccgc cgctcgcatc gcgcgaagat
     4561 cgtgaagcgc tcatcgccgg ggtcgccgat ggcgctgtgg atgtcatcgc taccgaccac
     4621 gcgccgcacg agcctgccct gaaggatgtt gagttcgacc gcgccccgtt cggcatcctc
     4681 ggtttcgaaa cggccatccc gctcgctctc gaacagctcg tcgccaccaa taaaattcct
     4741 ctcatgcgca tggtcgagtt gttcaccacc ggcccggcgc gcgtcctcgg catcgaacgc
     4801 aaactcgccg caggccagcc cgccgactta accatcttca tgacctcgca actgtggact
     4861 tacaacgtca acgattcgcc cagcaagtcc cgcaactcgc ccttcgacgg cagaaccttc
     4921 agcggcgcgc ccatggccac catcgtcgcc ggcaagctgg ccttccgccg ctaaaatcct
     4981 cgcagatcgc agcgttgcct ttcgcaaacc agccgctgca aacattctcc gttcaacggg
     5041 aatgcgctat tctttttgtc tgccttaacc acgaaaaacg gagcaggcaa gccgccaaca
     5101 tgcatgacct cagctacttt cgggaccatc tggacgtctt cgcggacatg gcgaagcgcc
     5161 gcaatatcac gctcgatctc gacaccttcc gcgcgattga caaggagcgg cgcgaattca
     5221 tcaccgccaa cgaacagcgc aaagcgcagc gcaacaaggc cagcgacgaa attgcccgcc
     5281 ggaaaaaaga taagctgaac gccgatgcgc tgatcgccga aatgaaacaa gtctccgagc
     5341 aaatcaaaca aagcgacgaa aaaatcagcg aactggaaac gaagcaacgc gatttgatgc
     5401 tggtggttcc gaatctgccg cacagctctg tgcccacggg aactgggtcg gaagacaacg
     5461 tggaagtgcg ccgttggggc acgccgccga aacttgattt tcaacctaag ccgcactggg
     5521 aggtcggcga acgcgccggc attctggatc tcgctgcggc gacgaaagtg accggagcgc
     5581 ggttcgcgat ttacaaaggc tggggcgcgc gcctggagcg tgcactggcg aatttctttc
     5641 tcgatgtgca tacccgagag cacggctaca cggaaattct gccgccgttt ctggtgaaca
     5701 ctgcttccct gatgggcgca ggacagttgc cgaaattcgc cgccgatctc ttcaaagtag
     5761 aaaacagtga cttctggctg acgcccacca gcgaagtcga attgcacaac ctgcatcgcg
     5821 atgaaacgct gccggaagac aaattgccca tcaacgtcac cgcgtggacg gcatgttttc
     5881 gctccgaagc cggcgcggcg ggtaaagata cgcgcggcat tctacgccag caccagttcc
     5941 agaaggtgga gctctttaag ttcacgcatc ccgaaaagag ttacgaagag cacgaaaaac
     6001 tcgttgccaa cgccgaaacc attctgcaaa agctcggact gcactatcgc agcatgctgc
     6061 tctgcaccgg cgatatgggt tttgcttcgg caaaaaccta tgacatcgaa gtgtggctgc
     6121 cgtcgagcaa cgaattccgc gaaatttctt cctgctccaa ttgcgaagcc ttccaggcgc
     6181 gccgagccgg aatccgcttc aagccaaagg ccggtggcaa gagcgaattc gtgcacaccc
     6241 tgaacggctc gggcttagct gtgggccgca cttggattgc cgtcgtcgaa aattaccaac
     6301 aggctgacgg ctcgatccta atccccgaag ctctgcgtcc ttatatgggt attgagcgca
     6361 ttccgtcggc atagagcagc cgcgtcttgc agaggtttag tcgtcgagga tttcgtcttg
     6421 gaatacggta agtttgcctt ccgcaaaggt cttgttctgg cattgttcct gattggtgtg
     6481 ctcgtactgg gaggacagag atggtttccg ggtgtttatc ggctttcgtt cttcttattt
     6541 ttggccggat cggcgctcgc ggcgtatctt cgcgctcggg aacggctatc ggaacgctcg
     6601 atgtccgagc aatctatacg tgcaccgggt gattggtccg cgaactctga gcgcctggtg
     6661 cgcgatccgg tttgtggact ttatctggct aagggttccg ccctgccgct acagcacgag
     6721 cttgagaccc tccctttctg ctccgcggtt gc
//
LOCUS       DASTPM010000477         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_247281, whole genome shotgun sequence.
ACCESSION   DASTPM010000477 DASTPM010000000
VERSION     DASTPM010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247281"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFR42_08465"
     CDS             complement(<1..726)
                     /locus_tag="VFR42_08465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0369227.1"
                     /translation="MSEYDVICIGAGPTGLATAIEAKRAGLRALVIDKGCLCNSIYHY
                     PVNMVFFTTPELLEIGDLPLVSPAEKPGRVEALKYYRKAAEHYELELRLYETVIRVEG
                     SNGDFTVHTRTEKGGPQKYQSRKIVVASGYYDLPNMLNVPGEDLPHVSHYYTEPHSFW
                     NQEVVVIGGKNSAAEAALDLYRNGVKVTLVHRQAHLGQTIKYWVKPDIENRIKAGQIK
                     ALLETTVKEITPEEVIVSSAGGEK"
     gene            complement(780..1115)
                     /locus_tag="VFR42_08470"
     CDS             complement(780..1115)
                     /locus_tag="VFR42_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369228.1"
                     /translation="MPELSPQQMKVVERLFAAGFRPIAIPPYESALCVRRGECAALLA
                     PVPNAGLRLLAAPTYIVDGNLSVKLKRAKGEVFVWKKTELEASPERLEELAKFRQELT
                     SILDEAALQ"
     gene            complement(1236..2096)
                     /locus_tag="VFR42_08475"
     CDS             complement(1236..2096)
                     /locus_tag="VFR42_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU0369229.1"
                     /translation="MLKRAGLPVTLKMRHDSHYVESLTSYSGAAIGRMIPVEQIRPNP
                     DQPRKSIGDLRDLTDSVREKGVLEPLLVRFVPREDCYYLISGERRYHAARAAGLREVP
                     CIEKTADDAETLEIGLIENIQRKDLTAFEEADGLHRLATQFEYTHEDVARKIGRARSS
                     VTETLSLLHIPESLRKKCIEQGILSKSLLLQIARQPTEKKMVEMLHRILQGGLTRDEA
                     RRERREEQSGPQRPQPFIFQFEPENETFKFRIQFKKSHVSRDELIVTLREILAQLEGN
                     SSGRADSTAA"
     gene            complement(2050..>2272)
                     /locus_tag="VFR42_08480"
     CDS             complement(2050..>2272)
                     /locus_tag="VFR42_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369230.1"
                     /translation="RDTHGQIRTVFGEVLFKTKISKNVRLEESPAYKETILTFSPKSP
                     GAVEYKKLAGEVIQRVEESRITRHAEDAA"
BASE COUNT          436 a          774 c          547 g          515 t
ORIGIN      
        1 cttttccccg ccggcgctgc tgacaatcac ttcttccggc gtgatctcct tcacggtagt
       61 ttccagcagc gccttaatct gcccggcctt gatccggttc tcaatatccg gcttcaccca
      121 gtacttgatg gtctgcccca ggtgcgcttg ccgatgcacc aacgtcacct ttacgccgtt
      181 gcgatataaa tccagcgccg cttccgccgc ggaatttttc ccaccaatca ccaccacttc
      241 ctgattccag aacgaatgcg gctccgtata ataatgcgaa acgtgcggca aatcctcgcc
      301 cggcacattc agcatgttcg gcaaatcgta atagccgctg gccaccacaa ttttccggct
      361 ctggtatttc tgcggcccgc ctttctccgt ccgcgtgtgc accgtaaaat cgccgttgct
      421 gccctccacg cgtatcacag tttcatacag tcgcagctcc aattcgtaat gctccgccgc
      481 tttccggtaa tacttgagag cctccacgcg ccccggtttt tccgccgggc tgaccagcgg
      541 caaatcgccg atttccagca gttccggcgt ggtgaaaaaa accatgttca ccggatagtg
      601 gtaaatcgaa ttacataagc agcctttgtc gatcaccagc gcccgcaagc ccgcgcgctt
      661 ggcctcaatc gccgtcgcca gtcccgtcgg gccggcgcca atgcagataa cgtcgtattc
      721 actcatagat tttcagtttg ccgggagtgc cgcagaattg caaaccgctc gccgtgacgc
      781 tattgcagcg ccgcctcgtc caaaatggac gtcagctctt gccgaaattt cgccagctct
      841 tccagtcgct ccggcgaagc ttccaactct gtcttcttcc acacgaaaac ttcgcccttg
      901 gcccgcttca atttcacgct caggttgccg tccacaatat aggttggcgc ggccagcagc
      961 cgcagtcccg cattcggcac cggcgccagc agcgccgcgc attccccgcg acgcacgcaa
     1021 agtgcgcttt catacggagg aatggcgatc gggcgaaatc ccgccgcaaa caaccgctcc
     1081 accactttca tctgttgagg acttagttcc ggcatgagtg gctcagcgac gcatcgctgt
     1141 gcgggcacaa tcccaagtta cgtggaccca agtcaacccg ctaaacagtc ggaccaaaat
     1201 cgcgaggtcg cttttctaac ggcgtgacgc cgacctcacg ccgcggtgga atccgcgcgc
     1261 cccgacgaat tcccttccag ctgtgccaaa atttcccgca atgtcacaat taattcatcg
     1321 cgcgaaacgt ggcttttctt gaactgaatc cgaaacttga atgtttcatt ctccggctca
     1381 aattgaaaaa taaacggttg cggccgctgc ggcccggact gctcttccct gcgctcgcgc
     1441 cgcgcttcat cgcgcgtcaa cccgccctgc aaaatccggt gcagcatctc caccattttc
     1501 ttttcggtgg gctgccgcgc aatctgcagc agcagcgact tcgacagtat cccctgctcg
     1561 atgcacttct tgcgcagcga ttccggaata tgcagcagcg acagcgtctc ggtcactgac
     1621 gaccgtgcgc gcccaatctt ccgcgccaca tcttcgtgcg tgtactcaaa ctgagtggcc
     1681 agccggtgca gcccgtcggc ctcttcaaac gccgtcaaat ccttgcgctg gatattctcg
     1741 atcaatccga tttccagcgt ctccgcgtcg tccgctgtct tttcgatgca cggcacttcg
     1801 cgcaagcctg ccgcgcgcgc cgcgtgatag cgccgctcgc ctgagatcag gtagtagcaa
     1861 tcttcgcgcg gcacaaagcg caccagcagc ggctccagca cgcccttctc gcgcaccgaa
     1921 tcggtcaagt cccgcaaatc gccaatcgac ttgcgcggct gatccgggtt cggacgtatc
     1981 tgctccaccg gaatcatccg cccgatggcc gctccgctat agcttgttag tgactcgacg
     2041 tagtgcgagt catgccgcat cttcagcgtg acgggtaatc cggctctctt caacacgttg
     2101 aataacctcc cctgccaact tcttgtattc gactgcgccg ggtgacttcg gcgagaacgt
     2161 caaaatcgtt tccttgtacg cggggctttc ttccagccgc acgttcttgc tgattttcgt
     2221 cttgaacagc acttccccga acaccgtgcg gatctgcccg tgcgtatccc gc
//
LOCUS       DASTPM010000478         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_160890, whole genome shotgun sequence.
ACCESSION   DASTPM010000478 DASTPM010000000
VERSION     DASTPM010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160890"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VFR42_08485"
     CDS             <1..1071
                     /locus_tag="VFR42_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369231.1"
                     /translation="SSSDAGPALPEITKDLEARSAKLFAAGGVAPVKSDTSGAENVDL
                     HDNYVKRLGELVRFEVLANAKAKFAVDTLHGCGAGYLNKVLADHGVPVETIRADRDVL
                     FDGTGPDVSEENLAPLRDVVLSAKATAGLATDGDADRFGIIDSDGSWVQPNLILALVY
                     DYLVEARGWRMPAARSVATSELVDAAAKSHGQTTFQTPVGFKYIGQLIREDKIALGGE
                     ESAGLTIRGHVPEKDGILACLLVAEMIAARGVSISEQIQQLFKKIGRSFAPVRENLHL
                     TDEQKAAAIQKSKTDASTLLGRKVKSVDRTDGAKFAFEDGSWILLRLSGTEPLLRLYV
                     EAENSAASAKLVREATDWILKG"
     gene            1075..1407
                     /locus_tag="VFR42_08490"
     CDS             1075..1407
                     /locus_tag="VFR42_08490"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU0369232.1"
                     /translation="MAAQDKLSMLWAHYGRALLGICVFGLFLHDIFGTHGFLAMRRTK
                     QEIERVQGEIARLNKENTELSQEVKALKSDPHKIESIARDELGLAKPGEVIIKIPKSE
                     QVKQQPAR"
     gene            complement(1424..>2153)
                     /locus_tag="VFR42_08495"
     CDS             complement(1424..>2153)
                     /locus_tag="VFR42_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369233.1"
                     /translation="AVCWLAGSGSPAAARNRSLAAGITASAAVALVTPGLWTIWRPKF
                     LPWPLESYINGVHIFNLPQPWLFPVFPWVAFAFAGLTVGFWMFSDLARKLDGWSFALL
                     GAAGVTICGLSVLFDRAGKWWFGTYDYWHTSPSFFLMRCGILLAILSFAYGWCRWGLA
                     RAGFSPLAQLGRTSLLVYWVHIEFVYGKFSILPKGQCSLPKATAGLVAIFTAMLALSL
                     FRTKWKRRKAGAAPSTQLQSSPAL"
BASE COUNT          509 a          610 c          658 g          376 t
ORIGIN      
        1 tcttcgtcgg atgccggacc ggcgctgcca gaaatcacca aggacctgga agcgcgatcg
       61 gcaaaactct ttgcggcggg cggcgtcgct ccagtgaaaa gcgacacctc cggcgccgag
      121 aacgttgact tgcacgacaa ctatgtgaag cgcctgggcg aattggtgcg ctttgaagtg
      181 ctggccaacg ccaaagcaaa attcgcggtc gatacgctac acggctgcgg cgcgggctat
      241 ttgaacaaag tgctcgctga ccacggcgtt ccggtcgaaa cgattcgcgc ggatcgcgac
      301 gtcttgtttg acggaaccgg ccccgatgtt agcgaggaaa atctggcgcc gcttcgtgat
      361 gtcgtgcttt ctgccaaagc gacagcaggg ctcgccaccg acggcgacgc ggatcgcttc
      421 ggcatcattg attcggacgg ctcctgggtt cagcccaacc taattctggc gctggtgtac
      481 gactatctgg tggaggcgcg cggctggaga atgccggcgg cacgcagcgt cgccacttcg
      541 gagctcgtgg atgcagcggc caagtcgcat ggacagacca cgtttcagac gccggtgggc
      601 ttcaaatata ttggccagct gattcgggaa gataaaatcg cgctgggcgg tgaagagagc
      661 gcggggctga ccattcgcgg ccacgtcccg gaaaaagacg gaatccttgc ttgcctgctg
      721 gttgcggaaa tgattgcggc acgcggcgtt tcgatcagcg aacagattca acagctcttc
      781 aagaaaatcg gccggagctt tgctcctgtc cgcgagaacc tgcacctcac tgacgaacaa
      841 aaggccgcgg cgatccagaa atcgaagacc gacgcttcca cgcttctcgg ccgcaaggtc
      901 aaatccgtgg atcgcaccga cggcgcgaaa tttgcctttg aagacggctc atggatactg
      961 ctgcgcctct ccggtactga gccgctactt cgtctctacg tggaagcgga aaattcggcg
     1021 gcgtcggcta aactggtgcg cgaagcaacg gactggattt tgaaaggctg aacaatggcg
     1081 gcgcaagaca aattgagcat gttgtgggca cactacggca gagcgctgct tgggatctgt
     1141 gttttcggat tgtttctgca cgacatcttt ggcacgcacg gcttcttagc gatgcgccgc
     1201 accaagcagg aaatcgagcg tgtgcagggc gaaatcgctc gcctgaacaa ggaaaacacc
     1261 gagctttcgc aggaagtgaa agcactgaaa tctgatccgc acaagattga atccatcgcg
     1321 cgcgacgagc tgggcttggc caagccgggc gaagtgatca tcaaaattcc aaaatcggag
     1381 caggtgaaac aacagcccgc ccgctgatcg ccgcgcccaa tcgttacaat gcgggagagc
     1441 tttgcaattg agttgaggga gccgcgccgg ctttacggcg tttccacttc gtgcgaaaca
     1501 gcgacagcgc cagcatggct gtgaaaatag cgaccaatcc cgcggtagcc ttgggcaggc
     1561 tgcactgccc cttcggtaga atggaaaact ttccgtagac aaactcgatg tgaacccaat
     1621 aaacgagcaa tgaagtcctg cccaactgcg ctagcgggct gaagccggcg cgagccaatc
     1681 cccagcggca ccagccatat gcgaacgaca ggatcgccag caggatgccg caccgcatca
     1741 ggaagaagct ggggctggtg tgccagtaat cgtaagtgcc aaaccaccac ttccccgcgc
     1801 ggtcgaataa aacggagagg ccgcagatgg tgacgccggc ggcgccaaga agagcgaagc
     1861 tccagccatc cagttttctc gcgagatcgc tgaacatcca aaaaccgacg gtgaggccag
     1921 caaaagcaaa cgcgacccac gggaacacag gaaagagcca gggctgaggc agattaaaaa
     1981 tgtgcacgcc gttgatatac gactcgaggg gccacggcag aaattttggc cgccaaatgg
     2041 tccacaggcc gggggtcact aaggcgactg cagcgctcgc agtgattccg gcggcgagac
     2101 tcctgttgcg cgcagctgcg ggtgagccgg agccggcgag ccagcaaacc gct
//
LOCUS       DASTPM010000479         5078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1400895, whole genome shotgun sequence.
ACCESSION   DASTPM010000479 DASTPM010000000
VERSION     DASTPM010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400895"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFR42_08500"
     CDS             <1..262
                     /locus_tag="VFR42_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitoxin AF2212-like protein"
                     /protein_id="HEU0369234.1"
                     /translation="LAWFRINHVYREQNREADALANQAMDDVSGAPRAENSKAKAEKG
                     NPPSAAIRVRARFRSGVLYPLEDVNLPDGVEVEILLRPASKN"
     gene            complement(273..755)
                     /locus_tag="VFR42_08505"
     CDS             complement(273..755)
                     /locus_tag="VFR42_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0369235.1"
                     /translation="MLQKIKPFLWFDTQAEEAAKFYCSVFQDSKTLNVERYPEGAPGG
                     MGGRVMIVNFQLEGLPFIALNGGPMFKFTEAISFSISTETQEETDYFWNKLTSGGGQE
                     SQCGWLKDKFGLSWQVVPIALSKLLSDPDKNKAQRVMQAMLQMKKIDIAALHRAAAHS
                     "
     gene            complement(808..1842)
                     /gene="mqnC"
                     /locus_tag="VFR42_08510"
     CDS             complement(808..1842)
                     /gene="mqnC"
                     /locus_tag="VFR42_08510"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEU0369236.1"
                     /translation="MPLTKQQALEMLQSDDLIGIGMEAHQLRMKKNDPRVATYQIDRN
                     INYTNFCTEYCSFCAFYRPIGAKDGYILSFEEIFKKIEEMLQLGGNGVLLQGGLHPDL
                     QIEYYENLLRSIKQKFPTVHLHCFSAPEILCIAEVSELSVEDTIRRLMDAGLASIPGG
                     GAEILDDEVRGRIARLKCTSDDWEMMHRTAHRLGLRTTATMMFGCGEELRHRVNHFER
                     IRRIQEDTGGFTAFIPWIFASDNTPLGRKVQETTAVDYLKTLAVSRLYLDNIDHIQSS
                     WLTPGIKVCQVGLQFGADDVGSILIEENVVYAAGVRNRTNESELRRVISDAGFIPAQR
                     DTLYRSYALK"
     gene            2296..3066
                     /locus_tag="VFR42_08515"
     CDS             2296..3066
                     /locus_tag="VFR42_08515"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0369237.1"
                     /translation="MGQTDLLNKEELEKLERRGLQVTVLSVVFVLVLAGGLAMFMYPL
                     VFEHPEGNKWTLRVAFFGFCGLTLLFVGYLLDQQKTLRQLKLQLMAEVERNIALKHQA
                     NVDLLGSMPDATQFFDRLTMEFRRAKAMEKTLSLVLLKVNPAKGTKPDMTAVCSAAAK
                     GMSRRLRPTDSIFRLAPELFGVVLPETDKMNGKRVAMRLQEELQAARAAFNVAFELSV
                     FNYPEDVVSAHEMEDLVKSLMPEQPGWDVAAPATALVE"
     gene            3208..4896
                     /locus_tag="VFR42_08520"
     CDS             3208..4896
                     /locus_tag="VFR42_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU0369238.1"
                     /translation="MQYLRTTLLLAMVSGSYLSACVAQSAPPVAAPAAGAIPSQSPSD
                     AKTQLIAKVEEKFASGEQNFKAGHLEAARRDFDAAVDLMLQSGYDVDRDPELHDLFRR
                     VVDTVYTWELQAFHAGDGFQETPAVPAPIDEVADMTFPVDPRLKDRAEVAARSFSHDL
                     PLTVNDEVLSFLNFFQTPRGKAIVETGLSRSGKYRDMISRILREEGVPQDLIYLAQAE
                     SAFQPAALSRAGARGIWQFVPWRGNEYGLKRSWWVDERQDPEKATRAAAQHLRDLYGL
                     FGDWYLAMAAYNCGPGNVQKGIERTGYADFWELYRRNVLPRETKNYVPIIVALTLIAK
                     DAAHYGIQADMEKPIASDVVKPGRAIDLRLVAETIDADVETLRMLNPSLLRMATPDDP
                     LFELRLPAGSATKFSAEIADIPAEKWVSWRRHRVEPGETLTSLAKKYKVTAAAIAAAN
                     SLGNSTALTSGDKLIIPATQPENETKSRLVRYRVRKGDTLGGIADQFSVSTDQLRKWN
                     ALKSGRVSRGMVLRVYTVGGAPEARPVRSRGTAKKKSSGVKTAASSPSATPKSP"
BASE COUNT         1040 a         1380 c         1604 g         1054 t
ORIGIN      
        1 actggcatgg ttccggatca accacgtata ccgcgaacag aaccgcgaag ccgacgcgct
       61 ggcgaaccag gcgatggacg acgtttccgg cgcgccgcgc gcggaaaaca gcaaagcgaa
      121 agcagaaaag ggaaatccgc cttccgccgc gattcgagtg cgcgcgcgct ttcgcagcgg
      181 cgtgctctat cctctcgagg acgtcaacct gcccgacggc gtcgaagtgg aaattctcct
      241 ccgtcccgcc tcgaagaatt agcgaatccc gcctacgaat gggcggcggc gcgatgcaga
      301 gccgcaatgt cgatcttctt catttgcagc atggcttgca tcacgcgctg cgctttgttt
      361 ttatcgggat cagaaagcaa tttgctgagc gcgatcggga ctacctgcca cgacaagcca
      421 aatttgtctt tcagccaccc acactgtgac tcctggccgc cacccgaggt cagcttattc
      481 cagaagtagt ccgtttcttc ctgtgtttcg gtggaaatgg aaaacgaaat cgcttcggtg
      541 aacttgaaca tcggcccgcc attcagcgcg atgaacggca gcccttcgag ctggaagttg
      601 acgatcatga cgcggccacc catcccgccg ggtgcgcctt caggataacg ctcgacgttt
      661 agggtcttcg agtcctggaa gaccgagcag tagaacttcg cggcttcctc tgcctgagtg
      721 tcgaaccaca ggaacggctt gattttttgc agcatggctt cgctttccac cagcgctttc
      781 tgccagagtt cgggtgagac ttctttttta cttcaatgcg taggagcgat acaaggtatc
      841 gcgctgggca gggatgaagc cagcgtcgga gatgacgcgg cggagctcgg attcgttggt
      901 gcggttgcgg acgccggccg cgtacaccac gttttcttca atcaggatgc tgccgacgtc
      961 gtcggcgccg aattgcaggc cgacctggca aaccttgatg ccgggcgtca gccagctgga
     1021 ctggatgtga tcaatattgt cgagatagag gcgactgacg gccagcgtct tcaagtaatc
     1081 gaccgcagtg gtttcctgaa cttttctgcc gagcggcgtg ttgtccgaag cgaaaatcca
     1141 cgggatgaag gcggtgaagc cgccagtatc ttcctgaatg cggcgaatgc gctcgaagtg
     1201 gttgacgcgg tggcgcagct cttcgccgca gccgaacatc atggtggcgg tagtgcgcag
     1261 cccgagtctg tgtgcggtgc ggtgcatcat ttcccagtcg tcgctggtgc atttgaggcg
     1321 ggcgattctg ccgcggactt cgtcatcgag gatttccgcg ccgccgccgg gaatggaagc
     1381 gaggccagcg tccatcaggc ggcgaatggt gtcttcgacg gaaagttcgc tgacttctgc
     1441 gatgcagaga atctccggtg cagaaaaaca gtgcaggtgc acggtaggaa atttctgctt
     1501 aatcgagcgc aacagatttt cgtagtactc aatttgcagg tcgggatgca ggccgccttg
     1561 gagcagcacg ccatttccgc cgagctgcag catctcttcg atctttttga agatttcctc
     1621 gaacgacaaa atgtagccgt ctttggcgcc gatgggccgg tagaacgcgc agaacgagca
     1681 gtattcggtg cagaaattcg tgtagttgat gttgcgatcg atctgataag tggccacgcg
     1741 cggatcgttc tttttcatgc gcaactggtg cgcttccatc ccgatgccga tcaggtcgtc
     1801 ggactggagc atctcgagcg cttgttgttt ggtcaggggc acaatgtttt ctctagcgtt
     1861 ctattctagc cgaagcgcgc gggcgattcc cgttttggca ggttcgtaaa gtttggagcg
     1921 gatggtcggg aactttgtcc cgaggctagg cccccagcgt cttgcgttag ttattgatta
     1981 caaaagactt accaggggct gtttctgcaa gcgtggattc taagggaact tagctggccg
     2041 ggccttcgat gacaacctca tattcaaggg taatagtacc gtataagata cgggaagtca
     2101 agcctgaaat ttggcggaac cgagccaggc ggactttgcc aactaagtcc tatcttttga
     2161 ggcgcaaaac gccaagttgt tgtttgcgct acacctgcct attggttcta ttgctcgcca
     2221 ccggctgcat tcccaatatg gaaattcaat gacagggaac cggtcgcggc gacaccgcgc
     2281 aggagcaagg aagaaatggg ccagacggat ttactgaaca aggaagagct ggagaagctg
     2341 gagcgccgcg gcctccaggt gacggtgctt tcggtggtgt ttgtgctggt ccttgcgggc
     2401 ggcctggcaa tgttcatgta cccgctggtt tttgagcacc ccgaagggaa caagtggacg
     2461 ttgcgggtgg cgttctttgg cttctgtgga ctaacgcttc tttttgtggg ttatctgctg
     2521 gaccagcaga agacgctgcg gcaattgaag ctgcagctta tggccgaagt cgagcgcaac
     2581 atcgcgctga agcatcaggc taacgtggat ttactcggct caatgccgga tgccacgcag
     2641 ttctttgacc ggttgaccat ggagttccgc cgggccaagg ccatggagaa gacgctttcc
     2701 ctggtgttgc tcaaggtgaa tccggccaag ggaactaaac cggacatgac ggcggtttgc
     2761 agcgcagcgg ccaagggcat gtctcgccgc ctgcggccca cggactcgat tttccgcctg
     2821 gcaccggaac tttttggcgt ggtgctgccg gaaaccgaca aaatgaacgg caagcgagta
     2881 gccatgcggc tgcaggaaga gctgcaggcg gcgcgcgccg cgttcaacgt ggcatttgaa
     2941 ttgagcgtct ttaactatcc ggaagatgtg gtgagcgcgc acgagatgga agacctggtg
     3001 aagtccctga tgccggagca gcccggatgg gatgtagccg cacctgcaac ggcgctcgtg
     3061 gaatagctcg aataaatgca caccgcggaa ttacctagac agttagattc ctcccatgcg
     3121 cggctggcca ttggtctggt ggccgatgag gaactcacca cactgaattc gttgacctgc
     3181 atgtaaaagc ctccgtacca tcaatacatg cagtacctgc gcactacgct gctattggcg
     3241 atggtttccg gctcgtattt gtccgcgtgt gtggcgcagt ccgctccgcc ggtggcagcg
     3301 cctgcggcgg gcgcaatccc ctcgcagtct ccttccgatg ccaagacgca acttatcgcc
     3361 aaagtagaag agaagttcgc gtcgggcgag caaaatttta aggccggcca tctggaagcg
     3421 gcgcggcgag attttgatgc ggccgtagac ctgatgctgc aaagcggcta cgacgtggat
     3481 agggatccgg agttgcatga tctctttcgc cgggtcgtag atacggtcta cacctgggaa
     3541 ttgcaggcct ttcacgcggg tgacggcttc caggaaactc cggcggtgcc ggctcccatc
     3601 gacgaagtcg cggatatgac gtttccggtg gatccgcggc tgaaagaccg ggcagaggtg
     3661 gcggccagga gtttttcgca cgacttgccg ctgaccgtga acgacgaagt gctctctttc
     3721 ctgaactttt tccagacgcc gcgtgggaag gcgatcgtag agacgggcct gagccggtcc
     3781 ggcaaatatc gcgacatgat ttcgcggatt ctgcgagaag agggggtgcc gcaggacctg
     3841 atttatctgg cgcaggcgga gagcgcgttc cagccggcgg ctctttcgcg ggccggcgcg
     3901 cgcggcatct ggcagtttgt gccgtggcgc ggcaacgagt acggtctgaa acggtcctgg
     3961 tgggtggatg agcggcagga tccggaaaag gcgacccgtg cggcggcgca gcacttgcgc
     4021 gatctctatg ggctgtttgg cgactggtat ctggccatgg cagcgtacaa ctgcgggccg
     4081 ggcaacgtac agaaaggcat cgagcgaacc ggctacgccg acttctggga gctgtaccgg
     4141 cgtaacgtct tgccgcgcga aaccaagaac tacgtgccga tcatcgtggc actgaccttg
     4201 attgccaagg acgccgcgca ctacggaatc caggcggata tggagaagcc gatagcgtcc
     4261 gacgtggtaa agccgggccg ggcgattgat ttgcggctgg tggcggaaac gatcgatgca
     4321 gacgtcgaga cgctgcgaat gctgaatccg tcgttgctac ggatggccac gccggacgat
     4381 ccgttgtttg aattgcgctt gccggcgggc agcgcgacga aattctcggc ggagattgcc
     4441 gatattccgg cggagaagtg ggtcagttgg aggcggcacc gggtggaacc gggggagacg
     4501 ctaacgtcgc tagccaagaa atacaaggtg accgcggcgg ccatcgcggc ggccaacagc
     4561 ctgggcaatt cgacagctct gacctcaggg gacaaactga tcattcccgc aacacagccc
     4621 gagaacgaaa cgaagagccg gctagtacgc taccgcgtgc gcaagggaga cacgctggga
     4681 ggcattgccg accagttcag cgtcagtacc gaccagctgc gcaagtggaa cgctctgaag
     4741 tcgggccggg tgagccgggg catggttttg cgggtatata cggtcggcgg cgcgcccgaa
     4801 gcgcggccag tccgcagccg agggaccgcc aagaagaaga gttccggcgt aaagacggca
     4861 gcctcgtcgc cgagcgcgac gccgaaatcc ccgtgatttg ccgcgtccgt tgcgtccgat
     4921 tcgcgcacaa cagaactctt gccgcagtga tgctcagaat ccttgacatt gcctgctgaa
     4981 tacggtacga acgccacagt ggttttgccg cgactgcggc ttttttgtgg ccgcgccggc
     5041 cccgatacgt cgggcaacag attcccccag gggaggat
//
LOCUS       DASTPM010000480         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1372146, whole genome shotgun sequence.
ACCESSION   DASTPM010000480 DASTPM010000000
VERSION     DASTPM010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372146"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFR42_08525"
     CDS             complement(<1..387)
                     /locus_tag="VFR42_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369239.1"
                     /translation="MLGKKIPKIIPPRSRVRLVRADNQTPSWKKDIGRQFRVGYYSRK
                     DGLDCIWLVNEGGAYEQNADRSSLLKYFDVEHLSQEKNFYGLGKRRLRKIRIPTPLER
                     LNGRSSIEAYEGAKESCKKTTPTWSAR"
     gene            complement(522..1220)
                     /locus_tag="VFR42_08530"
     CDS             complement(522..1220)
                     /locus_tag="VFR42_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2-related protein"
                     /protein_id="HEU0369240.1"
                     /translation="MSSPEIAPEIAHVTTPSARWKPLALRITITVVALAVWFWTQALL
                     GARPGGSEAIGDGLHRLTAPLHDYLTGSLRAANVLLIVSSLGIDALGIFLLGSWVFAG
                     KLRPFVGLTMLLILRQIMQALCALPPPPGMIWHYPGFPSLLVTYGVANDFFFSGHTAI
                     AVFGATELARLDKKWLTAFAIFLVLFEIAAVIVLRAHYTMDIFTGLLAAVCVAAISEP
                     VSRWIGPRERAAKT"
     gene            complement(1207..>2705)
                     /locus_tag="VFR42_08535"
     CDS             complement(1207..>2705)
                     /locus_tag="VFR42_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0369241.1"
                     /translation="RTAKLNFFSALTKTIFLARRLRPVWAEQKTVGVLLPPSIGGALV
                     NLAAMFAGKVPVNLNYTASQPVVDSCAQQSGLTVTITSRAFLERIKVQPPGKAVYVED
                     LAEKPGFGEKIVALLAALFFPARALEKLVGAEQKPSLDDIATIIFSSGSTGDPKGVLL
                     THFNVASNVEQLNQVFMLHHDDRILGILPFFHSFGFTGTLCLTTAVGIGAVYHPNPLD
                     ARTIGALVAKYRITLLLATPTFLRAYTKRCAPEDFGSLRYVIAGAEKLPAQVSEAFED
                     QFGIRPLEGYGCTECSPVVAVNTMDFRAAFFRQVGAKRGSIGHPLPGIVLRTVDPETQ
                     SVQPANQAGLLLVRGPNVMQGYLNKPEKTSEVLRDGWYNTGDIATIDEDGFVRITDRL
                     SRFSKIGGEMVPHIKIEEKLQELSAAPEQVFVVTAVPDEKKGERLIILHTLGDQALQE
                     VLSKLGETDLPALWKPRPDQFRKIETMPYLGTGKLDLRRVKELGNELA"
BASE COUNT          582 a          698 c          860 g          565 t
ORIGIN      
        1 ccgagcgctc catgtggggg tcgtcttttt gcaagattcc tttgccccct cgtacgcctc
       61 tatggaggac ctgccattaa gccgttccaa aggcgttgga atgcgtattt ttcgcagacg
      121 gcgcttccct agaccgtaaa agttcttctc ctgagacagg tgctctacgt cgaagtattt
      181 caacagggag cttcggtccg cgttctgctc gtatgctccg ccttcgttta ctagccagat
      241 gcaatccaat ccatctttgc ggctgtagta gcccacgcgg aattggcggc cgatgtcctt
      301 cttccaagaa ggtgtctggt tatcagctct gactaagcgg acccgtgaac gcggaggaat
      361 tatcttaggg attttcttgc cgagcatgtg aacgagaagg cccagtttct gcgaacgtcg
      421 gacgctattc tattcttata ggcggcgtag cggcaaagag gttgcgcaac tcctgattca
      481 tcggattacc ggcaatcaag tgaaatttct ggcgtggcgg atcaagtttt ggcggcgcgc
      541 tctctaggac cgatccagcg gctgaccggt tcgctgatcg cggcgacgca gacggctgcg
      601 aggaggccgg tgaaaatgtc catggtgtag tgggcgcgca ggacgatcac ggcggcaatt
      661 tcgaacaaga caagaaaaat ggcaaaggca gtgagccatt ttttgtcgag gcgggccagt
      721 tcggtggctc cgaaaacggc gatggcggtg tggccgctga agaaaaaatc gttggcgacg
      781 ccgtaggtaa caagtaggga gggaaatccc gggtaatgcc agatcatgcc gggtggaggc
      841 ggcagtgcgc agagagcctg catgatttga cgcaggatga gcagcatcgt aaggccgaca
      901 aatgggcgaa gttttccggc gaacacccag gagccgagca gaaaaattcc tagtgcgtcg
      961 attcctagcg aactcacgat gagcagcacg tttgcggcgc gcagcgagcc tgttaaatag
     1021 tcgtgcaggg gagcggtgag gcggtgcagg ccatcgccga tggcctcact accaccgggt
     1081 cgcgcaccga gcaacgcttg cgtccagaac cagaccgcga gcgcgacgac tgtaatggtg
     1141 atgcgcagtg cgagcggctt ccatcgcgcg gagggtgtgg tgacatgcgc aatttcgggc
     1201 gcgatttcag gcgagctcat tgccaagttc cttcacacgg cgcaaatcga gtttgcctgt
     1261 gccgagataa ggcatggtct cgattttgcg gaactgatcg ggacgaggct tccacagagc
     1321 gggcaagtcg gtttcgccaa gtttgcttaa gacttcttgc agcgcttggt cgcccagcgt
     1381 gtgcaagatg atcaagcgct cgcctttttt ttcgtcgggc acagcagtga cgacaaagac
     1441 ctgctccggc gcggcggaga gctcctgcaa tttttcttcg atcttaatgt gcggaaccat
     1501 ttcgccgccg attttgctga agcggctcag ccggtcggtg atgcgtacga agccgtcttc
     1561 gtcaatggtg gcgatgtcgc cggtgttgta ccagccgtcg cgaagcactt cggaagtttt
     1621 ttccggcttg ttgagatagc cctgcatgac gttgggaccg cgcaccagaa gcaatccggc
     1681 ttgattagcg ggctggaccg attgcgtctc gggatcgacg gtgcgcaaca cgatgcctgg
     1741 aagcggatgg ccgatgctgc cgcgcttggc tcccacctgg cggaaaaatg cggcgcggaa
     1801 atccatggtg ttgacagcga cgacgggtga acactcggtg cagccgtagc cttccagcgg
     1861 acggatgccg aattggtctt cgaaagcttc ggaaacttgc gcgggcaatt tttccgcgcc
     1921 ggcgatgacg tagcgcaggc tgccaaaatc ttcgggagcg cagcgtttgg tgtatgcgcg
     1981 cagaaatgtg ggcgtagcaa gcagcaaggt gatgcggtat ttcgcaacca gcgcgccgat
     2041 ggtgcgggcg tccagcggat tgggatgata aaccgcgccg atgccgacgg cggtagtgag
     2101 acacagcgtc cccgtgaaac cgaaggaatg gaaaaacgga agaatgccga ggatacggtc
     2161 gtcatgatgg agcatgaaaa cctgattgag ctgttcgacg ttggaagcga cgttgaaatg
     2221 cgtaagcagc acgcctttgg gatcgcccgt agagccgctg gaaaaaataa tagtggcgat
     2281 atcgtcgagc gacggctttt gttccgcgcc aaccagtttt tcaagcgcgc gggcagggaa
     2341 aaacagcgcc gccaagagcg caactatttt ttcgccgaag ccgggctttt cggcaaggtc
     2401 ttcgacgtag acggccttgc ccggcggctg gactttgatc ctttccagaa acgcgcgcga
     2461 ggtaattgtg acggtcaggc cgctttgctg cgcgcaagaa tcgacgaccg gctgcgatgc
     2521 cgtgtagttg aggttgacgg gaactttgcc ggcgaacatg gcggcgagat tgaccagcgc
     2581 gcctccgata gaaggcggga gaagaactcc gacagttttc tgctccgccc aaaccggacg
     2641 cagacgccgc gccagaaaaa tggttttggt caacgcggag aaaaagttga gcttcgccgt
     2701 acgga
//
LOCUS       DASTPM010000481         4605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_737888, whole genome shotgun sequence.
ACCESSION   DASTPM010000481 DASTPM010000000
VERSION     DASTPM010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737888"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(714..1847)
                     /locus_tag="VFR42_08540"
     CDS             complement(714..1847)
                     /locus_tag="VFR42_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369242.1"
                     /translation="MSAQSSPGPVNILMVDDQPAKLLSYEVMLEELGENLLKASSGRE
                     ALEMLLKTDVAVVLMDVSMPELDGFQLAEMIREHPRFQKIAIIFISAVHLTDLDRLKG
                     YQRGAVDYISVPVIPDLLRAKVSVFAELHRQARQLEVLNQELRRLSHSLMTAQDEERR
                     RISRELHDGLGQDLTAVKMMLGGLVQPNQLKDSTENKTIAEVLSMMDSAIEQVRTMSH
                     LLHPPLLDEMGLLSALRWYTEGFAKRSGVETVLDLPKNFPRLEPEIEMAIFKIVQESL
                     TNVFRHAEANRAVVQLEERDGCALVSIEDNGKGIGSGAEELHPNRYGIGVIGIKQRAA
                     EYDGQVTLRNTGSGTRVEVKIPLPVPPPQTVVPRFSKLTVRPI"
     gene            complement(1897..>4605)
                     /locus_tag="VFR42_08545"
     CDS             complement(1897..>4605)
                     /locus_tag="VFR42_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369243.1"
                     /translation="LKTNLARFTGMLQGQRELTTVGRMLLSELAPLVNAQQGVLYQME
                     AEEPRNMVLLSAFADSTEGHQEIVRVGEGLVGQCALEKRRMLISDLPDHTVPIRSGLF
                     EAVPRNVIVLPVLFEDRVKAVIELASLNSFTTSHLAFLEQLTSSIGIVLNSIEATMQT
                     ENLLEQSQQLATELQTQQRELQQTNEQLGLKAQQLAEQNVEVERKNQEIEQARRALEE
                     KAKELALTSKYKSEFLANMSHELRTPLNSILVLGQQLSENPEGNLSPKQVEFSRTIHG
                     AGTDLLNLISDILDLSKIESGTVSVQAEEVFFAGLLEVIARPFRHEADNRKLTFETYL
                     DSALSASLVTDAKRVQQVLKNLLSNAFKFTEQGKVRLSVLKAAGGWSADHPVLTRAES
                     VIAFEVTDTGIGIPAEKQRIIFEAFQQADAGTSRKYGGTGLGLAISRELANLLGGEIQ
                     LKSVAGSGSTFTLYLPQLYVGPTGAIPLTSQSSSHPTSPVRASAPDRVLERVPDDRDS
                     AQPGENILLIVEDDLHYARILCDLARDTGFKVLMASSGSEALTLAREFQPTAVSLDVF
                     LPDMLGWTVLNHLKQNPATRHIPVQMLTLDDDRRHGLSRGAFAFLTKPTDRSSLEAAF
                     AKIKQYSAPRRKRLLVVEDDPSEQFSIRELLGSDDIELEVAATGAEALDIVRKQTFDC
                     MVLDLRLPDMSGFEVLERLSAQGASADLPVVVFTGRELSLEEDTRLHELARSVVVKDV
                     ESPERLLDETSLFLHRVIAELPAEKQKMLDRLHHSDDALVGKRVLVVDDDVRNIFALS
                     SVLERRGMTVLSASTGREAIALLESTDDVAIVLMDIMMPEMDGYETMQVIRENAAFRR
                     LPIIALTAKAMKGDREKCLEAGASEYLAKPVNTEQLLSALRMWLHR"
BASE COUNT          945 a         1280 c         1352 g         1028 t
ORIGIN      
        1 gatgtgctct gattgcctct aaaaggcgga ccgttagaat gacttttgct gttccggaat
       61 cgagcaaccg ctggcgccgc gtgctccccg gcgcggcgaa ttcaaacctg ccgcaacccg
      121 cgttagctca ttgaaaatta aacgggttaa ccccagtctg taccaagcct cgctctttgg
      181 aacgccgcga ccacataaac cctagcagaa gcaatactta ctggcctctg caatgcgttc
      241 taaaggaact taactctctt tagtatcaat gcgtcaatac atacaagcga cgtacaagcg
      301 gctgctccat aagtcctctg gttcccatgc ttacaaaaaa aaggggtggg tggactcctc
      361 caggcgaatc tagcagtcag ctcagctcga tttcttgcta ggaaccttgg cgcctttctt
      421 gcgcgcctcc gatagcccaa ttgcgatcgc ctgcttccgg ctcttcaccc ggccgccctt
      481 tccgcccttg ccggaccgca gcgttccttt ctttcgccga cgcatcgccg acttcacgga
      541 cttctgagcc cctcttccgt atcgagccat ggttcacctc cggcccagac tactctttgc
      601 ggcaagcgcg attcgatgcc gcaattcctg taagcgcgaa gggacgttag ctgagtgctg
      661 gtttatttct ggagtctatc tggaagaagg gactgcgatg catttcgccg tagctatatg
      721 gggcgaacgg tcagcttgga gaagcgtgga acgacggtct gcggtggtgg aacgggcaag
      781 ggaatcttta cctcgacccg cgtgcccgat ccagtattgc gcagcgtaac ttgcccatcg
      841 tattccgcgg cgcgctgctt gataccaatg acgccgattc cataacggtt cgggtgcaat
      901 tcctcggcgc cgctcccaat gccttttcca ttgtcctcga tggaaacgag cgcgcagccg
      961 tcgcgctctt ccagttgcac cacggctcgg ttcgcctctg catggcgaaa gacgttggtc
     1021 agcgattcct ggacaatttt gaaaatggcc atttcgattt cgggttcgag gcgcggaaag
     1081 ttcttgggaa gatccagaac ggtttccacg ccgctgcgtt tggcaaagcc ttcggtgtac
     1141 cagcgcagcg cggaaagcag gcccatttcg tccagcaaag gaggatgcaa aaggtgcgac
     1201 atagtgcgca cttgctcgat cgcgctatcc atcatggaaa gaacttccgc gatggttttg
     1261 ttttccgtgg agtccttcaa ttgattgggt tgcaccaacc cgccgagcat catttttacc
     1321 gcagtcaggt cctggcccag gccgtcatga agttcgcgcg aaatgcgccg cctttcttcg
     1381 tcttgcgcgg tcatcaaact gtgcgacaaa cggcgaagct cctggttaag aacttccaac
     1441 tggcgcgctt ggcgatggag ttccgcgaag acactgactt tggcgcggag caaatcggga
     1501 atcaccggca ctgaaatata atccaccgcc ccgcgctggt agcctttcag ccgatccaaa
     1561 tcggtaagat gaaccgcgga gataaaaatg attgcaatct tctgaaaccg cggatgctcg
     1621 cgaatcattt ccgccagctg aaagccgtcc agttcgggca tgctgacatc catcaaaaca
     1681 accgcgacgt cagtctttag aagcatttcg agcgcttcgc gccccgacga ggctttcagc
     1741 agattctcgc caagctcctc cagcatgacc tcgtagctca gcagcttggc cggctggtcg
     1801 tcgaccatca ggatgttgac gggaccagga ctcgattggg cactcatctc tgcatcggct
     1861 ccgcggcttc gccggtcatt gcaaatcgga ataaaatcaa cggtgcaacc acatgcgcaa
     1921 cgcggaaagc agttgctcgg tattcaccgg tttggccaga tactcggatg cgcctgcttc
     1981 cagacatttt tcgcgatcgc ccttcatggc tttcgccgtg agcgcgatga taggtaaccg
     2041 gcgaaaggct gcgttttcgc ggatgacctg catcgtttcg tagccatcca tttccggcat
     2101 catgatgtcc atcagcacga tggccacatc gtcggtggat tccagaaggg caatggcttc
     2161 gcgaccggtg ctggccgaga gaacggtcat gccgcggcgt tccagaacgc tgctgagcgc
     2221 gaagatgttg cgcacgtcgt cgtcgaccac caggactctc ttgccgacaa gcgcgtcatc
     2281 ggagtgatgc aggcggtcga gcatcttttg cttttccgcg ggcagctcgg cgatcacgcg
     2341 gtggaggaac agcgaagttt cgtcgagcag acgctccggc gactcgacgt ccttcaccac
     2401 gacgctgcgc gccagctcgt gcagccttgt gtcttcttcc agagaaagtt cacgaccggt
     2461 gaaaacgacg actggcaaat ctgcagaagc tccttgcgcg cttaatcttt cgaggacttc
     2521 gaagccggac atgtcgggca agcggagatc cagaaccatg cagtcaaaag tctgtttgcg
     2581 gacaatgtcc aaagcttccg cgcccgtggc ggcaacctcc agttcgatat cgtcgctgcc
     2641 gagcagttcg cgaatgctga attgctcgga aggatcatct tcgacgacga gcaggcgctt
     2701 gcggcgcgga gcggagtatt gctttatttt ggcaaacgcc gcttccaggc tggaacgatc
     2761 cgtaggtttg gtcaggaaag caaacgcacc gcgggaaagg ccgtggcggc ggtcatcgtc
     2821 aagagtgagc atttgaacgg gaatgtggcg cgtcgcggga ttttgcttga ggtgattcag
     2881 caccgtccag ccgagcatgt ccgggagaaa aacgtccagc gaaaccgccg tgggctggaa
     2941 ttcacgcgcc agagtcaacg cttccgatcc gcttgaggcc atcaggactt tgaatccggt
     3001 atcacgcgcc aagtcgcaaa gaattcgcgc gtaatgcaga tcgtcttcga cgatcagcag
     3061 gatgttttcg ccaggctgcg cggaatcgcg gtcgtcgggc acgcgctcca gaacgcgatc
     3121 gggagccgaa gcccgtacgg gcgaagtcgg atgcgaagag ctctggctgg ttaacgggat
     3181 ggcgccggtg gggccgacgt aaagctgcgg caaatagaga gtaaacgtgc tgccggagcc
     3241 ggcgacactc ttgagctgga tttcgccgcc gagcaagttg gcgagttcgc ggctgatggc
     3301 gaggccaagt ccggtgccgc cgtacttgcg gctggtgcct gcgtccgcct gctggaacgc
     3361 ttcgaagatg atgcgctgtt tttccgccgg aataccaatg ccggtgtcgg tgacttcgaa
     3421 tgcgattacg gattcagcgc gagtgagcac gggatgatcg gcggaccatc caccggcggc
     3481 tttcagcacg gaaagacgaa ccttaccttg ttccgtgaac ttgaaagcgt tggaaagaag
     3541 attcttcaga acctgttgta cgcgcttggc gtcggtaacg aggctggcgc tgagagcgga
     3601 atcgagataa gtctcgaagg taagtttgcg gttgtcggct tcgtgacgga atgggcgagc
     3661 gatgacttcc agcaagccgg cgaagaacac ttcttcggct tgcacggaaa cggtgccgga
     3721 ttcgatttta gagaggtcga gaatgtcgct gatcaggttg agcaggtcgg taccggcgcc
     3781 gtgaatggtg cgggagaatt cgacctgctt gggtgaaaga ttcccttcgg gattttcgct
     3841 gagttgctgg cccagcacga ggatgctgtt cagcggcgtg cgcagctcgt gtgacatgtt
     3901 ggcgaggaat tcggatttgt atttcgaagt gagggcgagt tccttggcct tttcttccaa
     3961 cgcccgtcga gcctgttcga tttcctggtt cttgcgctcg acctcgacgt tctgttcggc
     4021 cagctgctgg gccttcaggc cgagctgctc gttggtttgc tgcaactcgc gttgctgggt
     4081 ctgcaattca gtggcgagct gctgcgattg ttcgagcaaa ttttccgttt gcatggtggc
     4141 ttcaatactg ttgagcacga tgccgatgct ggaggtcaac tgttcgagga aggcgagatg
     4201 agaggtagta aagctgttca ggctggccag ttcgatgaca gctttgacac gatcttcgaa
     4261 gaggacgggc aggacgatga cgttgcgggg aactgcctcg aatagtccgg aacggattgg
     4321 aaccgtatgg tccggcagat cggagatcaa catgcggcgt ttttccagcg cacattgccc
     4381 gaccaaaccc tccccgaccc ttactatctc ctgatggcct tccgttgagt ccgcgaaggc
     4441 agagagaagc accatgttgc gcggctcctc cgcctccatc tgatagagaa cgccttgctg
     4501 tgcgttgacc agaggcgcca gctcggacag cagcattcgt cctacggttg tcagttcgcg
     4561 ctggccctgc aacatgccgg taaagcgggc gagattggtc ttcag
//
LOCUS       DASTPM010000482         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_219208, whole genome shotgun sequence.
ACCESSION   DASTPM010000482 DASTPM010000000
VERSION     DASTPM010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219208"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFR42_08550"
     CDS             complement(<1..469)
                     /locus_tag="VFR42_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU0369244.1"
                     /translation="MSPLIGALMIRNLLAKSFLALAATTLLAATSPAQDRYKPSPENL
                     KARQDFQDDKFGLFIHWGVYSVLGDGEWVLHDRKLQLHDYERLPNFFNPEKFDAKAWV
                     ALAKAAGMKYITITSRHHDGFAMFDTKLSDWNIVQRTSYKKDPLKMLAEEAHRQ"
     gene            581..1156
                     /locus_tag="VFR42_08555"
     CDS             581..1156
                     /locus_tag="VFR42_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEU0369245.1"
                     /translation="MATTRLKFLQYVLLVTLCGGLLVEWARFRLAPSADRTAPAHLAS
                     LHDKLVGTWLLVSARDRMKDGSERFYPDLGAQGKGDLVYTADGHMCAQLMNPDRPKWK
                     EFDKPTEAEKISAIDGFSAYCGRYEVDEEKGMLVHLPELAWSPNYVGTRQLRPYTLQD
                     GLLTFAGKPLDEPEVESYSITWRRADTKKSQ"
     gene            complement(1185..1586)
                     /locus_tag="VFR42_08560"
     CDS             complement(1185..1586)
                     /locus_tag="VFR42_08560"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEU0369246.1"
                     /translation="MSATAKGTFDVKTTRQPAADFDHTGIARLLLDKQFHGDLDAVSK
                     GQMLAAATATKGSAGYVAIEKITGTLSGRKGSFVLQHIGTMNKGVPALKVSVVPDSGT
                     DQLTGLSGTMNIIIANGAHSYEFEYSLPGAP"
     gene            1973..>2567
                     /locus_tag="VFR42_08565"
     CDS             1973..>2567
                     /locus_tag="VFR42_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0369247.1"
                     /translation="MQTTATKEFVLFNADSRELRHWKLSIADRIATIAMNVDEEGGLR
                     PGYKLKLNSYDLGVDIELHNILQRIRFEHPEVASIVLTSAKERMFCAGANIYMLGTSS
                     HAWKVNFCKFTNETRNGMEDSSRHDGLKFLAALNGTAAGGGYELALACDEIILIDDRS
                     SAVSLPETPLLGVLPGTGGLTRVVDKRKVRRDLADMFC"
BASE COUNT          554 a          639 c          825 g          549 t
ORIGIN      
        1 cctggcggtg ggcttcctcg gcgagcattt tgagtggatc ttttttgtag gaagtgcgct
       61 ggacgatgtt ccagtcggag agcttggtgt cgaacatggc gaagccatcg tggtggcggg
      121 aggtgatggt gatgtacttc atgccggcgg ctttggcgag agcgacccag gctttagcgt
      181 cgaatttttc gggattgaaa aagttgggga ggcgttcgta gtcgtggagc tggagcttgc
      241 ggtcatggag aacccattcg ccgtcgccga ggacgctgta gacgccccag tggatgaaca
      301 ggccgaactt gtcgtcctgg aagtcctggc gggctttgag attttcgggg gaaggcttgt
      361 acctatcctg cgctggcgat gtggcggcga ggagggtagt ggctgccagc gcgagaaaag
      421 attttgcgag aaggttgcgg atcatcaggg ctcctatcag cggagacatt ttacactttg
      481 cgaaatgcga gcgaggaaaa agataacgga gaaatgtaga gagcgcggag agtaagattc
      541 gaccgagcgg ccgatccgtc gcggaactgt gaggagccga atggctacca caagactaaa
      601 gtttctgcag tacgtgcttc tggtcacgtt gtgtggcggg ttgctggtgg aatgggcgag
      661 gtttcgactc gctccttcgg cagaccggac ggcgccggcg catttggcgt cgctgcacga
      721 taagttggtg gggacgtggc tgctggtttc tgcgagagat cggatgaagg atggcagcga
      781 gcggttttat ccggaccttg gggcgcaggg aaaaggcgat ttggtataca cagcggatgg
      841 gcacatgtgc gcgcaattga tgaatccgga ccggccaaag tggaaagaat tcgacaaacc
      901 tacggaagcc gaaaagattt cggcgatcga tggattttcg gcgtattgcg ggcggtatga
      961 ggtcgacgag gagaaaggaa tgttggtgca cttgccggag ctggcgtggt caccgaatta
     1021 cgttgggacg agacagttgc ggccatacac gctgcaagac ggcctgctta cgtttgcagg
     1081 gaaacctctg gacgagccgg aggttgagag ttactcgatt acttggcgga gagcggacac
     1141 caagaagagt cagtgagctt tgcgaatcgt tggtggttac gcgcctaggg tgccccaggc
     1201 aaggaatatt caaattcgta ggagtgggcg ccgttggcga tgatgatgtt cattgtgcca
     1261 gagaggccgg tgagctgatc ggtaccggag tcgggaacga cggatacttt cagcgcgggg
     1321 acacccttgt tcatggtgcc gatgtgctgg agaacgaagc tgcctttgcg gccggagaga
     1381 gtgccggtga ttttttcaat agcgacatag ccggcggagc ctttggtagc ggtggcggcg
     1441 gcgagcattt ggcctttgct gacggcgtcg aggtcgccat ggaattgttt gtcgaggagc
     1501 aagcgggcga tgccggtgtg gtcaaagtct gcggcgggtt gccgagtggt tttgacgtcg
     1561 aaggtgcctt tggcggtggc ggacatgggg gttcccctgg tggtggatcc cgatggaacg
     1621 gtcgcagtcg gtacaagagt tgtcgcgaac aagagtgcgg ctaacgccaa gaaagacctg
     1681 gagtccatgg tgaggagagt gtagcgaaaa cgacgagcag acgcaccggc gaaatgtgtt
     1741 ttcttaacga aaggacgcag agagagcgga caatggccga tcagaggatt tgcaataggt
     1801 tccgcaccta cggcgctcgt ttcgtttgtt catagcactc gcggacgcgc cacgatcgcc
     1861 ccaatcgccg accgtccaaa cttctcgcca gcgaaccgga actatagttc cggtttgcat
     1921 ttccacacgg cgttatactc ctaagagagc tccgttgagg tctcccaacg caatgcaaac
     1981 gactgccacc aaagaatttg tcctcttcaa cgccgactct cgcgaactcc gccactggaa
     2041 actttccatc gctgatcgca tcgctactat cgcgatgaac gtcgacgaag agggcggcct
     2101 ccgccccggc tacaaactaa aactcaattc gtacgacctc ggcgtcgaca tcgaactcca
     2161 caatattttg cagcgcatcc gcttcgagca tcctgaagtt gccagcatcg tcctcaccag
     2221 cgccaaagaa agaatgttct gcgctggcgc aaacatctac atgctcggca cttcttccca
     2281 cgcctggaaa gtgaatttct gcaaattcac caatgaaacc cgcaacggaa tggaagattc
     2341 ttcgcgccac gacggcctga aattccttgc cgcgctcaac ggcaccgccg ccggcggcgg
     2401 ctacgaactc gctctcgcct gcgacgaaat tattctgatc gacgaccgct ccagcgccgt
     2461 aagcttgccc gaaactccgc tcctcggcgt tcttcccggc accggcggcc tcacccgcgt
     2521 tgtggacaaa cgtaaggttc gtcgtgatct cgccgacatg ttttgta
//
LOCUS       DASTPM010000483         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1083656, whole genome shotgun sequence.
ACCESSION   DASTPM010000483 DASTPM010000000
VERSION     DASTPM010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083656"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1757
                     /locus_tag="VFR42_08570"
     CDS             <1..1757
                     /locus_tag="VFR42_08570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0369248.1"
                     /translation="KTIAFLSARSGDSQVYVLSMEGGEAHPLTKLSTGADIVKWSPGG
                     KTILFTSSVFPECKDDACNKKRDEENEKNPVKAHVAENLLYRHWTHWNEGKRSHLFVM
                     AADGSGEAKDLTAGANYDVPPDQRGEDSDINFSPDSKEICFTAVTDKAEATSTNGDLF
                     TVPVSGGEPKRITAQTGFDGNPVYSPDGKYIAYHAQLAAGYEADRWRVMLYDRATGKS
                     ESLTEASFDRSAESLAWSPDSKTIYFLAENETLKPVYTMAAKAGATPKKLVDGFNAGF
                     SLSDDGKTLVLSRTTLSQPAELFVGASDGSGLKQITHANDALLAKLEMNVPETFWFDG
                     AEGARVQAMLIRPPNFDAKKKYPVLVLLHGGPQTMWGNSWGYRWNAQVFSAAGYATLM
                     INRRGSTGYGQKFTDDITNDWGGKAYVDVMKGVDASLAKFPFLDGTKMAAAGGSYGGY
                     MVDWIATHTDRFKALVSHAGVYNKVSMYATEELWFEEHDMQGTPWSNPESYRKFAPVT
                     YAGQLGKFKTPTLVIAGERDYRVPYTQSLEFFSALQRQGVPSKLVVFPDEGHWVMKPQ
                     NAKFWYKTFFAWVDKYTK"
     gene            complement(1895..2872)
                     /locus_tag="VFR42_08575"
     CDS             complement(1895..2872)
                     /locus_tag="VFR42_08575"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU0369249.1"
                     /translation="MITAEQTYLRTELEQRRRRLHTVLQSPAADASLSQLLTAVDDAL
                     RRIDQGTFGLCETCHESVEPERLLADPLVRFCLDHLTTTEQRALENDLSLAARIQRAL
                     LPNSALAPAGWDVRFHYQPAGMVSGDYCDFVETQSGLLFMLGDVAGKGVAASMLMSHL
                     HATFRSLAEADLPLDRMLGDANRIFCESTLAGQYATLVVGRAAHDGSVEFVSAGHLPL
                     LHIHAEGAAPKDSTGVPLGMFCSARFAVHRLTLAQGDSVLLYTDGLTEARNPAGQEYG
                     LPRIRALAARHTGKAPDELIYECLADLASFGQGLKQTDDLTLLVVRRAA"
BASE COUNT          732 a          877 c         1032 g          619 t
ORIGIN      
        1 gcaagaccat agcgtttctc tcggcgcgca gcggcgattc gcaggtgtat gtcctttcca
       61 tggaaggcgg cgaagcgcat ccgctgacca aactttccac cggtgcggac atcgtgaaat
      121 ggtcgccggg cggaaaaact attctcttca cttcttcggt ctttccggag tgtaaagacg
      181 atgcgtgcaa caagaagcgc gacgaagaaa acgaaaagaa tccggtgaaa gcgcatgtgg
      241 cggagaattt gttgtaccgc cactggacgc attggaatga aggcaagcgt tcgcaccttt
      301 ttgtgatggc cgccgatggt agcggcgaag cgaaagattt gactgctggc gccaactacg
      361 atgttcctcc cgaccaacgc ggcgaagaca gcgacatcaa tttttcgccg gactcgaaag
      421 aaatctgctt caccgcagtc accgataaag cggaagcgac cagcaccaac ggcgatctct
      481 ttaccgtgcc ggtgagcggc ggcgagccga agcgaatcac tgcacagact ggcttcgacg
      541 gcaatccggt gtattcgccg gacggaaaat acattgcgta tcacgcgcag cttgcggcgg
      601 gatacgaagc cgatcgctgg cgcgtgatgc tctacgatcg cgcgaccggc aaaagcgaaa
      661 gtctgacgga agcgtcgttc gatcgcagcg cggagagtct ggcctggtcg ccggacagca
      721 agacgattta ttttcttgcc gagaacgaaa cgctcaagcc ggtgtacacg atggcggcaa
      781 aagcgggagc gacaccgaag aagcttgtgg acggattcaa cgcggggttt tcgctcagcg
      841 acgatggaaa aacgttggtg ctttcgcgca ccaccttgtc gcagccggcg gaactgtttg
      901 tgggggcgag cgacggaagc gggctgaagc aaatcacgca tgccaacgac gcgctgctgg
      961 ccaagctgga gatgaacgtg ccggaaactt tctggttcga cggggcggaa ggcgcgagag
     1021 tgcaggccat gctgatccgg cctccgaatt ttgacgccaa gaaaaaatat ccggtgctgg
     1081 tgctgttgca cggcgggccg cagaccatgt ggggaaactc ttggggttac cggtggaatg
     1141 cgcaagtatt ttctgcggcg ggctacgcga cgctgatgat caaccggcgc ggctcgacgg
     1201 gttacggcca gaagttcacc gacgacatca cgaacgactg gggcggcaaa gcctacgtgg
     1261 acgtgatgaa gggcgttgat gcttcgttgg cgaaattccc ctttctcgat gggacgaaga
     1321 tggcggcggc cggcggatcg tacggcggct acatggtgga ctggatcgcg acgcatacgg
     1381 accgcttcaa agcactggtg agccatgccg gcgtgtacaa caaagtgtcc atgtacgcga
     1441 cggaagagct gtggtttgaa gagcacgaca tgcaagggac gccgtggtcg aatccggaga
     1501 gctaccgcaa atttgcgccg gtgacctacg cgggccagct gggcaaattc aaaacgccga
     1561 cgctggtgat tgccggagag cgcgactatc gcgtgccgta tacgcaatcg ctggagtttt
     1621 tcagcgcgct gcaaaggcag ggagtgccgt cgaagctggt ggtgttcccg gacgaagggc
     1681 actgggtgat gaagccgcag aacgcgaaat tctggtacaa gacgttcttt gcctgggtgg
     1741 acaaatatac caagtgagca atgactggac ggcccagccg tggctcgcgc gtcatggctc
     1801 gaacggcgga gcaacgaatc gggccgtccc gcctgtcgct ctcggccgga tttattggtg
     1861 gagggtggag gcgaagcgaa ttgaatagac ccggttacgc tgcgcggcgc acgaccaaaa
     1921 gggtcagatc gtcggtttgc ttcaagcctt gcccgaagct cgccaagtcg gcgagacatt
     1981 cgtagatcaa ttcatcgggc gctttcccgg tgtgccgggc cgcgagcgcg cgaattcggg
     2041 gaaggccata ctcctggccc gcgggatttc gggcttccgt caacccatcg gtatacagca
     2101 aaacgctgtc accctgggcc agggtcaagc gatggacggc gaagcgcgcg ctacaaaaca
     2161 ttcccagagg gactcccgtg gaatccttcg gcgcggctcc ctcggcgtgg atgtggagaa
     2221 gagggagatg gcccgcgctg acaaactcca ccgaaccatc gtgcgcagcg cgtcccacga
     2281 cgagagtcgc gtattgtccg gcgagagtgc tttcacagaa aatacggttg gcgtctccga
     2341 gcatgcggtc gagcgggaga tcggcttcgg cgagactgcg aaacgttgcg tggagatggc
     2401 tcatgagcat ggaggcagcc acgcccttgc cggcaacgtc tcccagcata aagagcaggc
     2461 cgctctgcgt ttcgacaaag tcgcaatagt cgccgctgac cataccggcg ggttgatagt
     2521 ggaagcgcac atcccatcct gctggcgcca gagcggaatt cggcaggaga gcacgctgga
     2581 tgcgcgcggc aagcgagaga tcattctcca gggcacgttg ttcagtggtc gtaaggtggt
     2641 cgaggcagaa ccgcaccagc ggatcggcca gcaaccgttc cggttctaca gactcatggc
     2701 aggtctcaca aagcccaaag gttccttgat cgatacggcg cagggcgtcg tccacagcgg
     2761 tcagcagttg agaaagagaa gcatccgcgg cgggcgattg caacaccgta tgaagacgcc
     2821 gacggcgttg ttcgagttcg gtgcgcagat aggtttgttc ggcagtgatc atatgatctc
     2881 ctgccacagg ttgctgcttt gcggccgagc tttccatccg atttcctcct ccgaattatc
     2941 gaggtccacg ggaagaaatt taacgggaac caaactgact ctcgctcttg aactcgcccc
     3001 gaagcaggaa cgagcaagcg gttccggtga gtcgtcagtg gtttggttag atgctgtaaa
     3061 catgcgacac ggtgcgggga atttgtagtc ctcacgattg agcgatcgcc tttttgcgac
     3121 tgacttgaag aaccaatatc tggcccactc accaggggtg aaactccacc caaggagtcg
     3181 attggctgaa atagagagaa aaacccgcaa ccttaaccat tttatcgcgt ccccgtttcc
     3241 gagcttttgc cgattcttgc
//
LOCUS       DASTPM010000484         6139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_74584, whole genome shotgun sequence.
ACCESSION   DASTPM010000484 DASTPM010000000
VERSION     DASTPM010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74584"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFR42_08580"
     CDS             <1..535
                     /locus_tag="VFR42_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0369250.1"
                     /translation="DGRALLEKAVQLQPDVVLLDLSMPILNGFEAGERLKKLLPRAKL
                     IVLTMTEDAEVAADVLHSWASGFLLKKSASAELVRAIQDVLRGKTFVTSHLAQNLLDK
                     FVRDPLQKSKTLTPRQREVLQLLAEGRTMKEVADVLNVTPRTVAFHKYKIMEEFELKT
                     NSDLLRFAMKERLVTRV"
     gene            682..2907
                     /locus_tag="VFR42_08585"
     CDS             682..2907
                     /locus_tag="VFR42_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0369251.1"
                     /translation="MTRLDWFALYLATASVLLFAASMLMTRSNGNSVVATLRKAEWLH
                     PGFREMTVDTMLLVFLVLEVLLLVWLWLEFPRRRRAEDALRKINLLQRAIARSSARIV
                     TMTSTEVAAGLHAELSGIRDMLGVDRICWYQQTADGKRFTRLQSASASVSLPGRDSFT
                     SNEHPWLASSILQGVPVLIGRLSDMPPGSELDRRNMEPSGIKSFALVPSLGSSGAANA
                     MILTSFSREIDWDAEVVAQLSVLASVFANAHARKLAQEAGTESESRFRHLFEEAPIGC
                     CLVNPQGLICLANGALKRMLGYGDDELIAKGFWEITSPEDVARSLVNFQELIAGVRDF
                     YQMEKRYVKKDGAEIWGRLTVSILGARSQEGQFVLAMVEDVTEAVRARAELQQSRRRL
                     IMALEASRMTAWEYDPANETIVWVDRNTLRDKAHEPAGPARFADVLHHVHPDDRSMLR
                     DLAIRIFNEGGVFSTEFRMFAKNGTIRWMLGKGELLRKADSSGTGKIVGVTLDVSELK
                     RTQVELQELAKRLMEAQEEERCRISRELHDDIGQRVALLGIELDLVRQLLHEQQPLRE
                     RVERAQLATGELGTDLHQLSHALHSSKLKYLGLPAALRELCTRMADKHSLAIELDCQE
                     HKLSLPEEEALALFRVAQEALNNVVRHSGATSVRVSLSQTAGELRLLVCDDGNGFDPA
                     AKTSGIGLIGMRERMRAVQGDFQIISAPGAATEIHATVTFPSPLPLGTVIRQLKAGTI
                     Q"
     gene            complement(3194..4705)
                     /locus_tag="VFR42_08590"
     CDS             complement(3194..4705)
                     /locus_tag="VFR42_08590"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0369252.1"
                     /translation="MPDIYGHFTEHLGGVMYDGLWVGPKSKVPNVDGLRRDVVEHMRT
                     IKAPVVRYPGGCFADSYDWRDGVGPADKRPRRTNFWNGAESEKSPANHKFESNEFGTN
                     EFVHFCKLIGAEPYLAANVRSLPAREFQHWVEYCNSPAGSTTLADVRAAAGYPEPFNV
                     RYWGVGNESWGCGGNFTPQEYAVEFRRYTAWLPQYGKPLSLIASGPNTDEWFWTREFL
                     QEIARKGPGQLREIYGMALHHYAWNLSRGKTNDWEKGKGDAIQFEPVDWYELLREGQR
                     MEGLIEGHWQVMGEFDREHRIKLIVDEWGPWYRAGSEATSGDILEQTPTLRDAVFSAL
                     TLDIFNRHCGKVAMANCAQLINCLNSLYLAHEDRFVVTTVGRAFELYAGHQGGQSLRT
                     EFSAPPVRYDRDGLQASFWGLNGSASVQGKQLLLTVVNPHVSEPREAEIVVRGGAIVS
                     GSVMSLTNSDIHARNTFEHRDAVMPVSNPLKMGGQLVHTFPPASASALTVQLR"
     gene            5510..>6139
                     /locus_tag="VFR42_08595"
     CDS             5510..>6139
                     /locus_tag="VFR42_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369253.1"
                     /translation="MRRHLIVFALCILPFSPARSATYYVDAKDGNDTNSGTSPGSAWK
                     SLEKINTSRFEPGDSILLKRGETWREQLNFPSSGAEGRPIVLDAYGTGELPVISGADT
                     IPSNSWNTSPTTNIWQASVATEPNVVIFDGAKGNKQTALEGLKQPMDWFWSAGTLYVF
                     APANPGQAFAKSGVEAGARLSAFNLSGKSYVAVRNIAVSGANAAPYALGS"
BASE COUNT         1298 a         1866 c         1680 g         1295 t
ORIGIN      
        1 agatggaagg gccctgttgg aaaaggcggt gcagttgcag cccgacgttg tcttgctgga
       61 tttgagcatg cccattttga atgggtttga ggccggtgaa cggctgaaaa agctactacc
      121 gcgggccaag ctgatcgtcc tgaccatgac tgaagacgcc gaggtagcag ccgacgtgct
      181 ccacagctgg gcttcgggct tcctcctcaa aaagtcggcc agcgcggaac tcgtaagggc
      241 cattcaagac gtgctgcgcg gaaaaacgtt tgtaacctcg cacctggcgc agaatcttct
      301 cgacaaattt gtacgcgatc cgctacaaaa atcgaaaaca ctgacgccca ggcaacgtga
      361 agttctgcaa ttgctcgcgg aaggccgcac catgaaggaa gtggccgacg tgctcaacgt
      421 aacaccgcgc accgtagcat tccataaata caaaatcatg gaagaatttg agctgaagac
      481 caattctgac ctgctacggt ttgccatgaa ggagcggctg gttaccaggg tgtagggcaa
      541 ctgccgccag agcccagttc tgtcgtggaa actgatcttt ggcgcgtagt cgccaaacgg
      601 attcttgcta gttgtgcaag gttaacgatg gcgcgttcga accttcttcg aatcgtttcc
      661 cgcccaggac tattcctgtc cttgacccgt ctggattggt ttgcgcttta tctggccacc
      721 gccagtgtcc tcctttttgc cgcctccatg ctgatgacga ggtcaaacgg caattcggtc
      781 gtcgccacac tccgcaaggc tgaatggctg caccccggct tccgtgaaat gacggtagac
      841 accatgctcc tggtcttcct ggtgctggaa gttctcttgc tggtgtggct gtggttggag
      901 tttccgcggc ggcggcgggc cgaagacgct cttcgaaaaa tcaatttgtt gcagcgcgcc
      961 atcgcgcgct cctccgcgcg tatcgtaacc atgacttcga ccgaagtcgc ggcgggactg
     1021 cacgcggaac taagcggcat ccgcgacatg cttggcgtgg accgcatctg ctggtatcag
     1081 cagaccgcgg acggcaaacg ctttacccgg ctgcagtccg caagcgcctc ggtaagccta
     1141 ccgggccgcg attccttcac gtccaacgaa cacccctggc tggcaagctc aattctgcag
     1201 ggcgtccccg tgctgatcgg acggctgagc gacatgccgc ccggctcgga gcttgaccgg
     1261 cgaaacatgg aaccctcggg aataaagtcg ttcgcgttgg ttccctctct cggcagcagc
     1321 ggcgctgcca acgcgatgat actgacttcc ttttcgagag agatcgattg ggatgctgaa
     1381 gtggtggccc agctgtctgt gcttgcttcg gtgttcgcca acgcccatgc tcgaaagctc
     1441 gcgcaggaag ccggcaccga aagcgaatcg cgcttccggc acttgttcga ggaagcacct
     1501 atcggctgct gcctggtaaa tccccaggga ctgatttgcc tggccaacgg cgccctgaag
     1561 cggatgctgg gctacggcga cgacgagctg atcgcaaaag gtttttggga aattacctcg
     1621 ccggaggatg tggccagatc gctggtgaat tttcaggagc tcatcgcggg cgtgcgcgac
     1681 ttctatcaga tggagaagcg ctatgtaaaa aaagacggcg cggagatctg gggccgcctc
     1741 acggtgtcaa ttctgggagc gcgctcgcag gaagggcaat tcgttcttgc catggtggaa
     1801 gacgtcacgg aggccgttcg cgctagagcg gaactccagc aaagccggcg cagactcatc
     1861 atggctctgg aagcttcgcg catgaccgcg tgggaatacg accccgccaa cgaaacgatt
     1921 gtttgggtgg atcgcaacac cctgcgcgac aaggcgcacg agccggctgg accagcgcgc
     1981 ttcgcggacg tcctccacca cgtccacccg gacgatcggt cgatgttgcg cgacctggca
     2041 atccgcatct tcaacgaagg cggagtcttc agcaccgaat tccgcatgtt tgccaagaac
     2101 ggaaccatcc gctggatgtt gggcaaaggt gagctgctgc gcaaagccga ctcaagtggc
     2161 accggaaaaa ttgtgggcgt caccctggac gtttccgagc tgaaacgcac tcaggtcgaa
     2221 ctacaagagc tcgcaaagcg attgatggaa gcgcaggagg aagagcgctg ccgcatcagc
     2281 cgcgaactgc acgacgacat cggccagcgc gttgccttac tcggaattga gctggacttg
     2341 gtgcgccaat tgctccatga gcagcagccg ctacgcgaac gggtcgagcg agcccaattg
     2401 gctaccggag agctcggcac ggatctgcat cagctctcgc acgccctcca ctcctccaag
     2461 ctgaaatacc tcggcttgcc cgcggcgctg cgcgaattgt gcacgcgcat ggctgacaag
     2521 cattccctgg ccatcgaact ggactgccaa gaacacaagt tgtctctgcc cgaggaagaa
     2581 gcgctggcgt tgttccgcgt agcccaggaa gcattgaaca acgtcgtccg tcacagcgga
     2641 gccaccagcg tccgcgtatc actgtcgcaa actgccggcg agttgcggct gctcgtctgc
     2701 gacgatggca acggattcga ccccgccgcc aagaccagcg gcattggttt aatcggaatg
     2761 cgcgaacgta tgcgcgccgt ccagggcgac tttcagatca tcagcgcccc gggggccgca
     2821 acggaaattc atgccacggt aacgttcccg tcgcctcttc cgttaggaac cgtgatcaga
     2881 caactgaaag cgggaacgat acagtagtgc ttttctgcta ggccatcaca aggccggcag
     2941 cgaactaatg tttttgtagg ttgccaccac cagccagcat gaatgacata tgaatttact
     3001 gccggctggt atttttgaca gttacacacg cttccagcgt ttgagataaa agtgggcgtc
     3061 gggcggcgga agttgcggcg cggcgctcaa tttgaaccaa attgagaaga cttccgccac
     3121 ccgtcttttt ttgacttgac cctcacacaa tcctctcgac aaaccatgct tgccttcagt
     3181 catggctcag cggttagcgc agctgcacgg tcagcgcgga agccgacgcg ggcggaaagg
     3241 tgtgcaccaa ctgaccgccc atcttcaggg gattactgac tggcattacg gcgtcgcgat
     3301 gctcgaatgt gttgcgcgcg tggatatcac tgttggtaag tgacatcaca cttcccgaaa
     3361 caattgcgcc tccgcgcaca acgatttccg cttcacgcgg ctcgctgaca tgcggattga
     3421 ccaccgtcag cagcagctgt ttgccttgca cagaagccga tccgttcaac ccccagaaac
     3481 tagcctgcaa cccgtcgcgg tcgtagcgaa ccggcggcgc tgaaaattcg gtacgcagcg
     3541 attgtccgcc ctggtgtccc gcgtacaact cgaacgcgcg tccaactgtc gtcaccacaa
     3601 accggtcttc gtgcgccaga tacaaactat tgaggcagtt gatcagctgc gcgcagtttg
     3661 ccatggccac ttttccgcag tggcggttga agatatccag cgtcagtgcg ctgaacaccg
     3721 cgtcgcgcag cgtgggtgtc tgctccagaa tgtcacccga agtcgcctca cttcccgccc
     3781 ggtaccacgg gccccactca tccacgatca acttgatgcg atgctcccga tcgaactcgc
     3841 ccataacttg ccagtgccct tcgatcagcc cttccatgcg ctggccttca cgcagtagtt
     3901 cgtaccaatc caccggttcg aactggatgg cgtcgccctt ccctttttcc caatcattag
     3961 tcttgccacg gctcaggttc cacgcgtagt gatgcagggc catgccgtaa atctcgcgta
     4021 gttgccccgg ccccttgcgc gcgatttcct gcagaaactc ccgcgtccag aaccattcat
     4081 cggtgttcgg tcccgacgca atcagcgaca gtggcttgcc atactgcgga agccaagccg
     4141 tgtaccgccg gaattccact gcatattcct gcggcgtgaa gttgccgccg caaccccacg
     4201 actcgttgcc cacgccccaa tagcgcacgt tgaacggctc gggatagccc gcagccgcgc
     4261 gtacatccgc cagcgtggtg ctccccgccg gagaattgca gtattccacc caatgctgaa
     4321 actccctcgc cggcaaactg cggacgttcg ccgccagata tggctcggcg ccaatcagtt
     4381 tgcagaaatg cacaaactca ttggtgccga attcattgct ctcaaatttg tggttcgcgg
     4441 gggatttttc gctttccgca ccgttccaga agttggtgcg ccgcgggcgc ttgtccgccg
     4501 gtccaacccc gtcgcgccag tcataactat cggcaaagca tcctcccgga taccgcacga
     4561 ctggcgcctt aatcgttctc atgtgctcta ccacgtcgcg gcgcaggcca tcgacgtttg
     4621 gcaccttgga cttcggtccc acccaaagcc cgtcatacat cacgccacca aggtgttcgg
     4681 tgaaatggcc gtagatatca ggcaaaattg tcccttgcgg ttcgtcgagc aaaacgtcaa
     4741 tgcgtgaatc cgccgtcggc gctgcaaccg catgtcttga agttgagaaa gtacctacgc
     4801 aggccgttgc tgtggttgcc agggccgtct tcacaaattc acgccgactt ttcacgctgc
     4861 acctcggttt cgaggggaat gaagatgcca gcagacacga gggttgtcaa actgaagcgc
     4921 ggagaactga aggaactgaa caaaccgcag tccagtccta cagcctccgc ggttaagaat
     4981 tgttaatttt cctgtatgtc acaatttccc gcgcccgcat acgctaacat agagaccccg
     5041 cccgctcagt tcgctcccaa tcggtttggc caggattacc atgtatgtcg gttcgtaagt
     5101 ccaatccctc ttcaggagag cgactcctcc tgctgatatt tgtcgccctg atcattattg
     5161 ccgccggacg ctgggccctc tccctatggt cccgccacta accccgtcca ggaatctgct
     5221 gtctgttcca acgctaattg tcttcctcca gtaagtcgtt tagaatctga tcgttgcgag
     5281 acgcataacg aaaccctttt aatgcttgct cttacaaaag tcatatttcc acaagcctcg
     5341 tattttcagt tatttgctgc gacacccggg atggggcttt tccaacaatc cgcgcacctt
     5401 agcttaggcc ggccacagaa ctctcagggt gttcctgtat caggccactt tttccgtcag
     5461 ccccgcttac tcacttgata aaatggaatt ctcagtttcg agggagctca tgcgaagaca
     5521 cctgatcgtc ttcgcgctct gcatcctgcc tttctcccca gcccgttcgg caacctacta
     5581 tgtagacgcg aaggatggca acgacaccaa tagcggtact tccccaggca gcgcctggaa
     5641 gtcgctggaa aagatcaaca cctcccggtt tgaaccggga gattccattc tcctgaagcg
     5701 cggcgaaacc tggcgcgagc agctgaattt tccgtcctct ggcgccgagg gtcggcctat
     5761 cgtcctggac gcgtatggca ccggcgagtt gccggtcatc agcggcgccg acacaattcc
     5821 ctcaaacagt tggaacacca gccctaccac caacatttgg caagccagtg tggccaccga
     5881 gcctaatgta gtgatcttcg acggagcgaa aggcaataaa caaaccgcgt tagaggggtt
     5941 gaagcaaccg atggattggt tttggagcgc aggcacgctc tacgtttttg cgccggcaaa
     6001 tcctggccaa gcattcgcca aatcaggcgt cgaagctggt gctcgtttga gtgccttcaa
     6061 tctcagcggc aaaagctacg tcgcagtcag gaacattgct gtctcgggcg ccaacgccgc
     6121 gccctacgcg ctcggctcg
//
LOCUS       DASTPM010000485         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1372416, whole genome shotgun sequence.
ACCESSION   DASTPM010000485 DASTPM010000000
VERSION     DASTPM010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372416"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..550)
                     /locus_tag="VFR42_08600"
     CDS             complement(26..550)
                     /locus_tag="VFR42_08600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0369254.1"
                     /translation="MRIRIALAAVLLAVFSTAAVPQAARPSDPKIIDAQAYQKLVKQH
                     VGKPLLMTFWATWCEPCRDEYPMLNQLAKEYEPKGLQVIGVNFDEDGDLILMRRFINR
                     YKPIFPNYRKKHGDEHGFTQAVWPEWNGALPASFLYDKDGNQVGRVLGAGTRETYENA
                     IRQLLAGSNNSASR"
     gene            732..995
                     /locus_tag="VFR42_08605"
     CDS             732..995
                     /locus_tag="VFR42_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEU0369255.1"
                     /translation="MAYVIAEPCIGTKDTACVDVCPVDCIHPRKDEPQFENEKQLYIN
                     PTECIDCGACVPVCPVTAIFALEDLPEKWQSYTPVNADWYAKK"
     gene            1162..>2158
                     /locus_tag="VFR42_08610"
     CDS             1162..>2158
                     /locus_tag="VFR42_08610"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU0369256.1"
                     /translation="MPPKFHLVLLIHAHQPCGNFGHVFEQSFERCYLPFVEALEKHPS
                     VHVGLHYSGPLLTWIEENRAAYFQRLKTLVKNGQVEMVGGGFYEPILVSIPDADQRVQ
                     LIRLADYIEHHFGERPSGAWLAERVWEPQLASVLAEATIGYTVLDDIHFLSAGFEPEE
                     LFADYIAEDQGKTIRLIPGQKILRYLIPFGTIEQVITYLKETANAHPDQMAAMGDDME
                     KFGVWPGTFEHCYKDGWLENFFVALEENASWLKAATPSEYLATHTPAGRADLPTASYT
                     EMMEWALPTRVRQRYNQVLKEFGSRNEVLAFLRGGSWRGFFRKYAESNLLHKKMLH"
BASE COUNT          459 a          572 c          645 g          482 t
ORIGIN      
        1 ggaccggagc ggcgcagggc gtttcttaac gggaagcgct gttgttcgac ccggcaagca
       61 actggcggat tgcgttttcg taggtttcgc gagtgcctgc gccaaggacg cggccaactt
      121 gatttccgtc cttgtcgtag aggaaggagg cgggtagcgc gccattccat tcgggccaaa
      181 cggcttgggt gaatccgtgc tcatctccgt gttttttgcg atagttcggg aaaatgggct
      241 tgtagcggtt gataaagcgg cgcatcaaga tcaaatcgcc atcctcgtcg aaattgaccc
      301 caatcacttg cagccccttt ggctcatact ccttggccaa ttggttgagc atagggtact
      361 catcgcgaca gggctcgcac caggtggccc agaaggtcat gagcaggggt tttccgacat
      421 gttgcttgac caatttttgg tatgcctggg cgtcgatgat tttggggtcg gagggacggg
      481 ccgcttgagg aacagcagcg gtggaaaaca cggcaagcag gactgcggca agggcaattc
      541 ggattcgcat tacattcctt tccctaccat tctatctata gacgcgccaa gttgagaaac
      601 ctattccccc cgcgtacaat caagctatgc tgcttgttgc ccccgaaggg gccgtggtat
      661 aagaaatttt tcgctcgtcg cgcactccgt acgtagtgcc tccgggccgc gctcgaacaa
      721 aggagaattg aatggcttac gtgatagccg aaccctgcat cggcacgaaa gatacggcgt
      781 gcgtggacgt gtgcccggtg gactgcatcc acccgcgtaa ggatgaaccg cagtttgaaa
      841 acgagaagca actttacatc aacccgaccg agtgtatcga ctgcggcgca tgcgtgccgg
      901 tttgcccggt taccgctatc tttgcgctgg aagacctgcc ggaaaaatgg cagagctata
      961 cgcctgttaa cgccgactgg tacgccaaga agtaactgga tttcccagcc tccagggcgg
     1021 catcgatctg gcgttgccgc atcgttcaag atgcagtaag catccaaaag gggtgcgtaa
     1081 tacgcgcact tcgtctctgt cgaagcattg taactttgag cggcagagtt attctgtttc
     1141 tctggagatc gccccgtccc cgtgccgcca aagttccatc tggtcctcct tatccacgcg
     1201 catcagcctt gcggtaattt cgggcatgtt ttcgagcaat cttttgagcg ctgctacctg
     1261 ccctttgtcg aagcgctgga aaagcatccg agcgttcacg tggggctgca ttattcgggg
     1321 ccgctgctga cgtggattga agagaatcga gcggcctatt tccagcgcct gaagacgctg
     1381 gtgaagaatg gccaagtgga gatggtgggc ggcggcttct acgagccgat tctcgtttcc
     1441 attccagacg ccgaccagcg agtgcaactc attcggctgg cggactacat tgaacatcat
     1501 ttcggcgagc gcccgagcgg ggcgtggctt gcggagcggg tatgggagcc gcagctggct
     1561 tcggtgcttg cggaagcgac tatcggctac accgtgctgg atgacatcca ctttctgtcg
     1621 gcgggtttcg agccagagga actttttgcc gattacatcg cggaggatca agggaaaacg
     1681 atacgactca tccccggtca aaagattctg cgttatctga ttccgttcgg caccatcgag
     1741 caggtcatca catatttgaa ggaaaccgca aacgcacacc cagaccaaat ggcggccatg
     1801 ggcgacgaca tggagaaatt tggcgtgtgg ccggggacct ttgagcattg ctacaaagac
     1861 ggctggctcg aaaacttttt tgtggcgctg gaagaaaacg cttcctggct gaaagctgcg
     1921 acgccgagcg aatatcttgc gacgcacacg ccggcgggac gcgctgatct acctaccgct
     1981 tcttatacgg agatgatgga gtgggcgctg ccgacgcggg tgcgccagcg gtacaaccag
     2041 gtgctgaagg agttcggttc gcgcaacgaa gtgctggcgt ttctgcgcgg cggctcgtgg
     2101 cggggattct tccgcaaata tgccgagtca aacctgctgc acaagaaaat gttgcatg
//
LOCUS       DASTPM010000486         3836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1228210, whole genome shotgun sequence.
ACCESSION   DASTPM010000486 DASTPM010000000
VERSION     DASTPM010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228210"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFR42_08615"
     CDS             complement(<1..608)
                     /locus_tag="VFR42_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HEU0369257.1"
                     /translation="MLSPRIRRLKLDHDQLQKRFAGWPLIQITGTAGMPPEVYRFVYH
                     VKGLIVGPNGEIQERPTHHLEVNLSLGYPRRAPQCRMLTPVFHPNFDEATVCIGDFWA
                     ASEGLDDLIIRIGRMIAYQEYNTKSPLNGLAAKWSEQHAHLFPVDARDIAPPLEKSKT
                     DAAAPPIVAPNPSEAPTSAAVSSPAPASEQSTPPSASQATTTG"
     gene            complement(630..1016)
                     /locus_tag="VFR42_08620"
     CDS             complement(630..1016)
                     /locus_tag="VFR42_08620"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEU0369258.1"
                     /translation="MTSARSAANLAATGAAAGAAMLYRFSPQEYSFYPRCPFFALTHH
                     YCPGCGATRALAELLHGSFTAALHFNAAVTVLLPFVLCYFAKMYWTALRENRVEWASI
                     PDWSWRVAIAGLAIFAVTRMFTQGIL"
     gene            complement(1013..1696)
                     /locus_tag="VFR42_08625"
     CDS             complement(1013..1696)
                     /locus_tag="VFR42_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEU0369259.1"
                     /translation="MNYFIKRDLNEYGPYSLADLQKYVASGNILLTDQCRSEGLMDWV
                     PVSQVIGNIPVPAAAQPAPAAPAAYAAPAVSPYPPPPNLHWGICLLLSIVTCGIFSVV
                     WIMVEALWVKKVRPASKAVTYFAIVIGLYVLYLVLSISGAVSAARTGHTDSSLQGIQG
                     IISLGILIMWIVGAFNMRDAIEEHFNSAEPIGLSLSGVMTFFFNVWYFQYHFTKINEM
                     KRRQMGYTV"
     gene            complement(1723..2388)
                     /locus_tag="VFR42_08630"
     CDS             complement(1723..2388)
                     /locus_tag="VFR42_08630"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HEU0369260.1"
                     /translation="MNPVCPYCRAEVGDAEGERKDCPGCDTPHHADCFAENGGCTVFG
                     CSQAPADEAKVTVTATDMAGNTVTRQVTPPTPRPGVSLGAGFTTFYAPGVEVPSGGSQ
                     AASNVAGSALPPPPPPLVGTGVAPPPQAGPAPYAATYAPAQLASYYQPPQPKTRVLFG
                     LLGIFLGALGVHNFYAGYTKKGAIQLCLTLLTCFYGAAISWPWAIIEICVINKDAEGN
                     QFV"
     gene            complement(2385..3449)
                     /locus_tag="VFR42_08635"
     CDS             complement(2385..3449)
                     /locus_tag="VFR42_08635"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0369261.1"
                     /translation="MSPENNDKTIKITLDDLANVSAPEMDAVAPATQSGSAKSYGSIN
                     EPADPAAQVAEEKGSILLQAWFYLGLAGLLGALIGWGVCEPWFVDSPTGGHFWADFLM
                     IPAIVGLLCLGFGVAESIVERSAKKALIRAAICLPLGAVLGVVFEFIANIIYSIGISI
                     CVEMGAQSNHNPALWVARGIAWMVFGAAGGVVYGTAGMSGKKVLYGVLGGVLGAGLGG
                     ILFDPIAIATHGGAVSRAVGFALVGMATGIAVGLVESALKDRWLYVTAGPLAGKQFIL
                     YKPRTVMGSDQSCDIYLFKDTNILKEHAIVELAGSRIMLRAIGQTYVSGQPVRQQVLM
                     SGAVVQIGRYAFRYQERQRN"
     gene            complement(3433..>3836)
                     /locus_tag="VFR42_08640"
     CDS             complement(3433..>3836)
                     /locus_tag="VFR42_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369262.1"
                     /translation="EKEGRSEVGPPAWLGWVITVCSHVLLMALAFVAAWILFPRLIPV
                     YFFIDPYKGVAIPAPTGTHLNSNDTLSIPGFSDIPVLRPEQLPQPKAPAGTPGANRPD
                     AAPANPPQPAQPKGGDSKDSKDQKDQNVPRK"
BASE COUNT          814 a         1171 c         1083 g          768 t
ORIGIN      
        1 cccgtagtag tggcttggct cgctgagggt ggcgttgatt gttcggacgc cggtgctggc
       61 gagctaacgg ccgctgaagt tggtgcttcg gagggattag gcgccacaat cggcggcgcg
      121 gcggcatccg ttttcgactt ttcgagcggc ggcgcaatgt cgcgcgcgtc caccggaaaa
      181 aggtgcgcat gctgttcgga ccacttggcc gccaggccgt tcagtggact ttttgtgttg
      241 tattcctggt aggcaatcat gcggccgatg cggatgatca ggtcgtcgag tccctcggag
      301 gctgcccaaa aatccccgat acacacggtg gcttcgtcaa agttgggatg gaaaaccggt
      361 gtcaacatac ggcattgcgg cgcgcggcgc ggatagccga gggaaagatt gacttccaga
      421 tggtgcgtcg ggcgttcctg gatctcgccg ttcggcccga cgatcagccc ttttacgtgg
      481 tacacaaatc ggtagacttc cggtggcatt ccggccgtgc cggtgatttg gatgagcggc
      541 cagccggcaa agcgcttctg cagctgatcg tggtcgagtt ttaaacggcg gatgcgcggc
      601 gaaagcataa gcgtctgagc cccgtgaaat tatagaattc cttgcgtgaa cattcgcgtg
      661 actgcaaaaa tcgccaagcc agcaatggca accctccagc tccagtctgg gatggacgcc
      721 cactcgacgc ggttttcgcg cagagcggtc caatacatct tagcgaaata gcagagtacg
      781 aagggcagca gcactgtcac agcggcattg aaatggagcg cggcggtgaa gcttccgtgc
      841 aagagttcgg ccaaggcgcg ggtcgcacca cagcctgggc aataatgatg ggtcagcgcg
      901 aagaacgggc accgcgggta gaaggaatac tcctgcggtg agaaacggta caacatcgca
      961 gccccggccg ctgctcccgt cgcagcaaga ttcgccgcac ttcgcgcaga agtcataccg
     1021 tgtagcccat ctgacggcgc ttcatctcat taatcttcgt gaagtgatat tggaagtacc
     1081 aaacattgaa aaagaacgtc atgacgccgc ttaaactcag tccgatcggc tcggcggaat
     1141 tgaagtgctc ctcgatggcg tcgcgcatgt tgaatgcgcc cacgatccac atgatgagga
     1201 tgccgaggga aatgatgccc tgaattcctt gaagagaact atccgtgtgt cccgttctgg
     1261 ctgcggaaac tgcacccgaa atgctcaaca ccaagtacag gacataaagc ccgatgacga
     1321 tagcgaaata agtgacggct ttgctcgccg gtctaacctt tttgacccat agtgcttcga
     1381 ccatgatcca caccacagaa aatatcccgc aggtcacgat cgagaggagc aggcaaattc
     1441 cccagtgcaa attcggcggc ggaggataag gtgaaacggc cggcgcggcg tacgccgctg
     1501 gcgcagcggg cgccggctgt gcagcagcag gcaccggaat gttgccgatg acttgagata
     1561 ccggcaccca atccatcaga ccctcgctgc gacattggtc ggtcagcaga atattccccg
     1621 acgcaacata tttttgcagg tccgccagcg agtaaggccc gtattcattc aggtcgcgtt
     1681 taatgaaata gttcatcgcg cagccctccg tcgcaaagag tcttacacaa actgattgcc
     1741 ttccgcgtcc ttgtttatta cacaaatctc gataatggcc cacggccagc tgatggcagc
     1801 gccgtaaaag cacgtcagca aagtcaggca aagctgaatc gcgccttttt ttgtgtagcc
     1861 cgcataaaaa ttgtgcacgc cgagagcgcc caggaaaatg cccagcagcc caaacagaac
     1921 acgagtcttc ggctgtggtg gctggtagta gctcgcaagc tgtgctggcg cataagtcgc
     1981 cgcgtaaggc gccggccccg cctgtggcgg aggcgctacg cctgtaccca cgagtggcgg
     2041 aggaggcgga ggcagagcgc tccctgctac attcgaagca gcttgcgaac cgccgctcgg
     2101 gacttcgaca cccggagcat agaaggtggt aaatcctgca ccaagcgaaa cgccggggcg
     2161 cggcgtgggc ggagtcacct gccgcgtgac cgtgttaccg gccatgtccg tcgcggtgac
     2221 tgtgactttt gcctcgtcag ccggtgcctg cgaacaaccg aagaccgtgc atcccccgtt
     2281 ttccgcgaaa cagtccgcat ggtgcggcgt gtcgcagccc gggcagtcct tgcgctcgcc
     2341 ctctgcatcg cctacttccg ctcggcaata cgggcagacc ggattcaatt ccgttgcctt
     2401 tcctggtagc gaaatgcgta gcgaccaatt tgcaccaccg ccccgctcat cagaacctgc
     2461 tggcgcacag gttggccgct gacgtaagtc tgtccaatcg cccgcagcat aatccgcgaa
     2521 cctgccagct cgacgatggc atgctccttc aggatgttcg tgtctttgaa taagtagatg
     2581 tcgcagcttt ggtcactgcc catcaccgtg cgcggcttgt acaagataaa ctgcttgccc
     2641 gccagcggcc cggccgttac gtacagccag cggtccttca gcgcgctttc caccagtcct
     2701 acggcgattc ccgtagccat gcccaccaat gcgaagccca ccgctcggct taccgctccg
     2761 ccgtgcgtcg cgatcgcaat cgggtcaaac aatatgccgc ccaaccccgc gcccagcact
     2821 ccgcccagca cgccatagag cactttctta ccggacattc ccgccgttcc gtacaccacg
     2881 ccaccggctg ctccgaaaac catccaggcg atgcctctcg cgacccacaa agcggggttg
     2941 tgattgcttt gtgctcccat ctccacgcaa atgcttattc caatggaata aattatgttg
     3001 gcaatgaatt caaaaacgac gcccagcact gctccgagcg gcagacaaat cgctgctcga
     3061 atcagcgcct tttttgcgga ccgctcgacg atgctttcgg ctacgccgaa tcccaggcac
     3121 agcagcccca caatcgcagg gatcatcaga aaatctgccc aaaaatggcc gcccgtcggg
     3181 ctgtccacga accacggctc gcagactccc cagccgatca gcgccccgag caaccccgcc
     3241 agccccagat aaaaccacgc ttgcagcaga atgctgcctt tttcttccgc cacttgcgcc
     3301 gccggatctg ctggctcgtt gatcgacccg taactcttgg cgctgccact ctgcgtcgca
     3361 ggcgctaccg cgtccatctc cggcgcggag acattcgcca gatcatccag cgtgatcttg
     3421 atcgtcttgt cgttattttc tggggacatt ttgatccttc tggtccttcg aatccttgct
     3481 gtcgccgccc tttggttgtg ccggttgcgg cggattggcg ggcgcggcgt ccggtcgatt
     3541 cgcaccaggc gtgcctgcgg gagccttggg ttgcggaagt tgctcagggc gcaaaacagg
     3601 aatgtcgcta aatcccggaa tactcagcgt gtcgttcgaa ttcaagtgcg tcccggtcgg
     3661 tgcaggaatc gccactcctt tgtacggatc gatgaaaaag tagaccggta tgagccgcgg
     3721 gaaaaggatc caagccgcca caaacgccag ggccatcagc aaaacgtgcg aacaaaccgt
     3781 gatgacccag cccagccagg ccggcggtcc cacttccgat cggccttctt tttctt
//
LOCUS       DASTPM010000487         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1141728, whole genome shotgun sequence.
ACCESSION   DASTPM010000487 DASTPM010000000
VERSION     DASTPM010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141728"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFR42_08645"
     CDS             complement(<1..556)
                     /locus_tag="VFR42_08645"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369263.1"
                     /translation="MPSIGPEELLARLKKGKPIPALLLLGEETYLRDSCRAALIDAFV
                     SEAARTWAISRFSADRGEVKSALDQAQTLPMLSPQQVVFLEEAESIEEFSDKKREDAV
                     KQLEAYLADPASFTVLVVEAAHLDQRMRLGKILAEKALVVQVGLGDNQDQRNAAAVAL
                     ARSLAREQGIDFERGAAEDLAESVA"
     gene            complement(556..1101)
                     /gene="lptE"
                     /locus_tag="VFR42_08650"
     CDS             complement(556..1101)
                     /gene="lptE"
                     /locus_tag="VFR42_08650"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEU0369264.1"
                     /translation="MSRARAVLILIATAALAGCGYHVAGRNDALPKTVHVIAVPALEN
                     KTASYRIEQRLTAATIHQFLAATPYKIVSSDSGADAVLRGKVLAVEAVPLLFITTPAT
                     ATQPSTTRATTMLITMKCEVTLTEAATEKVLYHNDNFVFRNEYEISTDVKSFFEEQDP
                     ALDRMAQDFAKRLVAAITENF"
     gene            complement(1098..2108)
                     /locus_tag="VFR42_08655"
     CDS             complement(1098..2108)
                     /locus_tag="VFR42_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HEU0369265.1"
                     /translation="MPTPLESRRVVIAGASSLLGAELKSLLEESRFAASDFRLVDEET
                     AAGTLTEAGGEAAVIQPVEEDSFDKAWIIFFTGSSAFTKTNLPLAKRSGAHIVDLSGE
                     LAGQSGVHTWLPKVSTLPTGGRPSSFAIPSASAEIIARLAISLSSLKPSVISAVAFQP
                     VSAAGKPGIEELESQTGQLLSFQSVGKQIFDAQVAYTMLDRYGASSGQSLSAALAVTR
                     REVQALQPPAAKLPAIQLLHAPVFYGSTVAICAFLDASPDTGTIAKLSGEVGFTVTDE
                     AIPPSNVSSAGEIFIQLAPPSPDPSAPGAFWFWAAADNLRLPASNAVKLAEKLLDQKP
                     FE"
     gene            complement(2111..2962)
                     /gene="pssA"
                     /locus_tag="VFR42_08660"
     CDS             complement(2111..2962)
                     /gene="pssA"
                     /locus_tag="VFR42_08660"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEU0369266.1"
                     /translation="MAEINQQEIPFEGRRNRMQARLRRGIFLLPAMMTSANLLCGYYA
                     VVASVLGTPEDFNHAAKAIGIAIIFDSLDGRVARLTGSNTEFGVQFDSLADVVSFGVA
                     PAVLAYSWGVRSVANLDPGALRQLGHFGWLCCLAFLICCAWRLARFNVQGMAPGGSKY
                     FVGMPTPASAGVIASIVHAFHVGSPLVDVGSISDWRWSVPWLLLAVSLGGLMTSTIRF
                     YSFKDLPWGKKQPAFLIILTLLLLAVIWNFSEIALLVLASTYAIAGVTLHVVRLLRHR
                     MVSRTAP"
     gene            complement(2965..3633)
                     /locus_tag="VFR42_08665"
     CDS             complement(2965..3633)
                     /locus_tag="VFR42_08665"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEU0369267.1"
                     /translation="MVKEGYYFGVPPLLLGGFAIIFHWWILGSLLIALGFFCFSFFRD
                     PERAIPDDPAAIVSPADGRVVVITEEQNAGRPGTRISIFLAIWNVHVNRSPAAGVITK
                     LDYRPGKFLAAWDANASAQNEQNIFTLATAAGTIEFKQIAGLVARRVVSWNKPQDVVT
                     RGQRIGLVRFGSRVDLWIPQGSQLLVKVGDTVYGGSSTLARWPVATRLSRTEEQAHPA
                     ETHN"
     gene            complement(3795..>4133)
                     /locus_tag="VFR42_08670"
     CDS             complement(3795..>4133)
                     /locus_tag="VFR42_08670"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEU0369268.1"
                     /translation="VGTNPAQGKCFYPHVSVRPGAVEKLNRRKEEERMAALQRSPRDI
                     LLDTDADYQRLVEQHHKYDEELQRIIKEPYLSSEDLLEEIKLKKLKLHCKDEMERIAS
                     RIQRARAQHA"
BASE COUNT          888 a         1066 c         1393 g          786 t
ORIGIN      
        1 cggctacgga ttcagccaga tcttcggcgg cgcctctttc gaagtcaatt ccctgctcac
       61 gagcgagcga cctggccaga gcgacggcgg cggcgttgcg ttgatcctgg ttatcgccga
      121 gacctacctg aacgacgaga gctttttccg ccaggatttt gccgagacgc atgcgctggt
      181 cgaggtgcgc ggcttcgacg acgagaacag taaaagatgc ggggtcggca agataggcct
      241 cgagttgctt gacggcgtct tcgcgttttt tatcgctaaa ttcctcgatc gattcggctt
      301 cttccagaaa gacgacttgc tgcggcgaga gcatgggcag ggtttgcgcc tggtcgaggg
      361 cggacttcac ctctccgcga tcggccgaaa aacgggagat ggcccaggtg cgggcagctt
      421 cagagacaaa cgcgtcgatc agtgcggcgc ggcaagaatc gcgcaggtag gtttcttcgc
      481 cgaggagaag aagcgcgggg atgggcttgc cttttttgag gcgcgccagg agttcttcgg
      541 ggccaatgga tggcatcaga agttctccgt gatggccgcg acgaggcgct tggcgaaatc
      601 ctgcgccatg cgatcgagag cgggatcctg ctcttcaaag aagcttttta cgtcggtaga
      661 tatttcgtat tcgttgcgga agacgaagtt atcgttgtga tagaggacct tttcagtggc
      721 ggcttcggtg agggtaacct cacacttcat ggtgatcagc atagtggtgg cgcgcgtggt
      781 ggagggttgt gtggctgtgg cgggcgtggt gatgaaaagg agcggcacag cctcgacggc
      841 gagaaccttt ccgcggagga cagcgtccgc accgctatcg ctggagacaa ttttatatgg
      901 cgtggcggcg agaaactggt gaatggtcgc ggcggtaagg cgctgctcga tgcgatagct
      961 ggcggttttg ttttcgaggg cgggaacggc gatgacgtgc acggttttgg gcagagcgtc
     1021 gttgcggccg gcgacatgat agccgcagcc tgcgagggct gcagtggcga tgagtattag
     1081 aacagcgcgg gcgcggctca ttcgaacggt ttctggtcca aaagtttttc ggctaatttt
     1141 accgcgttcg aagccgggag gcgaaggttg tcagcggcgg cccagaacca gaatgcgcca
     1201 ggggcggatg gatcgggcga gggcggagca agttggatga aaatttcgcc ggcggagctc
     1261 acgttgctgg gaggaatggc ctcgtccgtc acggtgaaac caacctcgcc ggaaagtttg
     1321 gcgatcgttc cagtatcggg agacgcgtca aggaatgcgc aaatcgcgac ggtcgatccg
     1381 taaaacacgg gagcgtgcag aagttgtatg gccggcaatt ttgcggcggg cggctgaagg
     1441 gcttgcactt cccggcgagt aacggcaagc gcggcgctga gcgattggcc gctggaggcg
     1501 ccgtagcggt cgagcatggt gtaggcgact tgcgcgtcaa agatttgttt gcccacggac
     1561 tggaaagaga gcagctggcc ggtttgcgat tccagctctt cgatgccagg cttgcccgcg
     1621 gcggacaccg gttgaaaggc caccgcgctg ataacggagg gcttcagcga actgagggag
     1681 attgcgaggc gcgcgatgat ttcggccgat gctgatggaa tggcgaagga gcttggccgc
     1741 ccgccggttg gtagcgttga gactttcggg agccatgtgt gaaccccgga ttgtccggcg
     1801 agttcgccgg aaagatcaac gatgtgagcg ccgctccgtt tggccagcgg gagattggtt
     1861 tttgtgaaag cagaagaacc cgtgaagaag atgatccagg ccttatcgaa gctgtcttct
     1921 tcgactggct gaatgaccgc ggcttccccg cccgcttcgg tgagcgtgcc ggctgcggtt
     1981 tcctcgtcga caagacggaa gtctgaagca gcgaaacggc tttcttccag aagcgacttc
     2041 aattcagcgc cgagcagcga cgatgcgccg gcaatgacga cacggcgcga ttcaagaggg
     2101 gtgggcatgg tcagggagcg gtgcgagaaa ccattcggtg gcgaaggaga cgaacgacat
     2161 gaagggtcac cccagcgatg gcgtaggtgg acgcgagcac gagcagcgcg atctcggaaa
     2221 agttccagat aacggccaac agtagcaagg tgagaatgat gaggaaggcc ggctgttttt
     2281 tgccccaagg caagtctttg aagctgtaaa aacggatggt gctggtcata aggccgccga
     2341 gcgagacagc gagcaaaagc cagggaacgg accagcgcca gtcggagatg gagccaacgt
     2401 cgaccagcgg actgccaacg tggaacgcgt gcacgatgga ggcgatgacg ccggcggatg
     2461 ctggcgtggg cataccgaca aaatatttgg agccgccggg agccatgccc tgcacgttga
     2521 agcgagccag acgccacgcg cagcaaatca ggaaagccag gcagcagagc catccgaaat
     2581 gcccgagctg gcgaagagcg ccgggatcga gattagcgac gctgcgaact ccccacgagt
     2641 aggcgaggac agcaggcgcg acgccaaagc tgacgacgtc ggcaagcgaa tcgaactgaa
     2701 cgccaaattc ggtgttgctg ccggtgaggc gcgcaacgcg gccatccagc gaatcaaaaa
     2761 tgatggcgat gccgatggct ttggcagcgt gattgaaatc ttcgggcgtg ccgagcacgg
     2821 acgccacaac agcgtagtag ccgcaaagca agttcgccga ggtcatcatg gccggtagca
     2881 ggaaaatgcc acgccgcaga cgggcctgca tgcgattgcg gcggccttcg aaggggattt
     2941 cttgctgatt gatctcagcc atggtcagtt atgtgtctcc gcggggtgcg cctgttcttc
     3001 ggtgcgtgat aaacgagtag caaccggcca acgggcaagc gtgctggaac cgccatacac
     3061 cgtgtctcct acttttacca gaagttgcga gccttgcgga atccacaaat cgacgcgcga
     3121 gccgaaacgg acaaggccga tgcgctggcc cctggtaacg acgtcctgcg gcttgttcca
     3181 ggagactacg cggcgagcga ccaggcccgc gatttgcttg aactcgatgg ttccggccgc
     3241 agtggccaag gtaaaaatgt tctgttcgtt ttgcgccgag gcgttggcgt cccaagcggc
     3301 gagaaatttt ccggggcgat agtcgagttt ggtgatgacg ccggcggccg gagaacggtt
     3361 gacgtgaacg ttccagatgg cgaggaagat agagatgcgc gtaccggggc ggccggcatt
     3421 ttgttcctca gtgatgacga ccacacggcc atcggcgggc gatacgatcg cggcgggatc
     3481 gtcggggatt gcgcgctcgg gatcgcgaaa gaaggaaaag caaaaaaatc caagagcgat
     3541 taggagcgac cccagaatcc accagtgaaa tatgatggca aaaccgccaa gaagtaaggg
     3601 cgggacgccg aaatagtagc cttctttgac catgcgctaa tgaagatacc acagcgcagc
     3661 cggcaggcca ggcgtgctgc cgtcgcgatc atcacgcggc cggggcttgc gcaaaaaaaa
     3721 gagcgcgaca aaagacaaat cacggccgtg atggacaacg tttggacttc gcggcgagag
     3781 gcgcgcgcta cgagtcaggc gtgttgcgcg cgcgcgcgtt ggatacgcga agcgatccgc
     3841 tccatctcgt ctttgcagtg gagcttcaac tttttcagct tgatttcttc caggaggtct
     3901 tccgaactga ggtagggttc tttgatgatg cgctgcaact cttcgtcgta cttgtgatgc
     3961 tgctccacca gacgctgata atcggcgtct gtatcgagga gtatgtcccg agggctccgc
     4021 tgcaatgctg ccatgcgttc ctcctcctta cggcgattca atttttcgac tgcaccgggc
     4081 cgcaccgaaa cgtgcgggta aaagcacttc ccctgtgccg ggttggtccc gac
//
LOCUS       DASTPM010000488         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_536547, whole genome shotgun sequence.
ACCESSION   DASTPM010000488 DASTPM010000000
VERSION     DASTPM010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536547"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFR42_08675"
     CDS             <1..725
                     /locus_tag="VFR42_08675"
                     /inference="COORDINATES: protein motif:HMM:NF037386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0369269.1"
                     /translation="DTLAAHPRLAKYMDMPLQHASRNVLARMKRGSHGDAFLLLLERI
                     RTTIPAVTLRTSFIVGFPGETEKDFDELCDFVQAGKFDWMGVFEYSDVDHAGSFALDV
                     KVDPEIITDRRKRLMAIQKKISREKLRSWKGRTEVALLEGPSPDNPMVWEARLQGMAP
                     EIDGRLYLTDIELSDGSLADTGDVVRVEIIKTDAYDLIGKVVEILPRPAKRPLPASPP
                     PPRQVHETLHRITTGAPLRVLR"
     gene            735..950
                     /gene="yacG"
                     /locus_tag="VFR42_08680"
     CDS             735..950
                     /gene="yacG"
                     /locus_tag="VFR42_08680"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEU0369270.1"
                     /translation="MKHRCPICKSATDSDAHAEFPFCSERCRLLDLGAWASEKYVVSD
                     PVFDEEELAEAERRTSDTRKQENETIH"
     gene            934..1632
                     /locus_tag="VFR42_08685"
     CDS             934..1632
                     /locus_tag="VFR42_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEU0369271.1"
                     /translation="MKPSTDAARQKPSLALAIATACGVGYLPKAPGTFGSLVGILTAM
                     ATALFFLRPHSLRDLLSTRRLTESTLMDHNFLVPGAEIHNAALVLPVVSAILLVLLLS
                     FVGVWSAGKAAAYAGLKDPQHVVIDEVAGQHITLILPLLPIAVPNLATHMDFSTYAIF
                     SALSMLNWKYLLAGFILFRLFDIWKPYPIGNLEKLQGGWGIMADDWLAGVYAAIPLKV
                     ALHFGLFAFHLGSS"
     gene            1629..2675
                     /locus_tag="VFR42_08690"
     CDS             1629..2675
                     /locus_tag="VFR42_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEU0369272.1"
                     /translation="MKFGLGSSEKNGIPRIEGVSPSAAIPGGEVEIRGSGFVSRTFAR
                     PQVHFGEAEASVAMSGPQRLVVRVPEGASGGAVRVAAGSHESQPFPVSVGLQIADNLH
                     PVANPAVDAEGNTYVTFSGPRGQRVPVSLYKITANFNVKPFVTSLTNPSGLALDRAGN
                     LFVSCRNDGTIHRVSPDGRAEQWIEGMGVATGLTFDREQNLYVGDRSGTIFKISPSRE
                     IFVFATLEPSVAAYHLAFHPTGELYATGPTTSSFDRVYRISQGGEVTTFFRGLGRPQG
                     IAFDRDGNLYVAASLGGRRGIVRISQMGRAELVLSGGAIVGMALLPSGRAMVATSNAI
                     FTLDWDVRGTPLFG"
     gene            2749..4011
                     /locus_tag="VFR42_08695"
     CDS             2749..4011
                     /locus_tag="VFR42_08695"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU0369273.1"
                     /translation="MKAEIIAVGSELLTPDRQDTNSLFLTAELNKLGIEVVRKTIVGD
                     ERALLLEAFRDALNRVPIIIACGGLGPTEDDVTRETVAELLGRKLVRNDAVVQSIEAR
                     FRSFGRQMTENNLRQAMVPEGAEPLENPRGTAPGLWLEKGDQAIALLPGPPRELKPLF
                     LEQVLPRLQRRAPAGKMFHRELRVTGLGESHVDHLAGPIYTRYPEVQTTILASPGEVQ
                     LHLRIWSGDTRHATRTLADLEQALDLALAERIFSRDGSPLEVVVARDLLKHCATISAA
                     ESCTGGLLSERLTTIAGSSAYFLGSVVCYSNDLKTAWADVPTEMIAAKGAVSPEVAVA
                     LAQGIRRRVGSTLGVGITGIAGPGGGSEEKPVGTVHIAVASPAGVRERTARFPGDREM
                     IRMQASQAALDMVRLHFLYNGQAPPARS"
     gene            4025..>4225
                     /locus_tag="VFR42_08700"
     CDS             4025..>4225
                     /locus_tag="VFR42_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU0369274.1"
                     /translation="MALQLSVEVRETFAALLGELRRADPKPRWAEPENLHITLKFIGH
                     IADEELPLICHALAAVPVPPPMT"
BASE COUNT          800 a         1404 c         1159 g          862 t
ORIGIN      
        1 tggacacgct ggccgcgcat ccccgcctgg cgaagtacat ggacatgccg ctgcagcacg
       61 ccagccgcaa cgtccttgct cgcatgaagc gcggctcgca cggcgacgcc ttcctccttt
      121 tgctggagcg catccgcacc acgattcccg ccgtgacgtt gcgcacctcc tttatcgtcg
      181 gcttcccagg cgagaccgaa aaggattttg acgagttgtg tgacttcgtt caggccggca
      241 agttcgactg gatgggcgtc ttcgaatact ccgacgtgga ccatgctggc agcttcgcgc
      301 tcgacgtaaa ggtcgatcca gaaatcatca ccgaccgtag aaagcggctg atggccatcc
      361 aaaagaaaat ctcccgcgaa aaactgcgtt catggaaagg ccgcaccgaa gtcgccctgc
      421 tggaagggcc gtcgccggac aacccgatgg tctgggaggc gcgcctgcaa ggcatggcac
      481 cggaaatcga cggtagattg tatctaaccg acatcgaact ttcggacggc agtttggccg
      541 acacggggga cgttgtccgc gtcgaaatca taaagaccga cgcctacgac ctgatcggca
      601 aggttgtcga aatcttgccg cgtccggcaa agcgccctct tcccgcctcg ccgccacctc
      661 cgcggcaggt tcacgaaacg ctgcaccgca tcacaactgg agcgcctctg cgcgttttgc
      721 gataagctgg cgccatgaag catcgctgcc ccatctgcaa atccgcaacg gactcggacg
      781 cccacgcgga gttccccttc tgcagcgagc gttgccgttt gcttgacctg ggcgcctggg
      841 ccagcgaaaa atacgtcgta agcgatcccg tctttgacga ggaagaactc gccgaagctg
      901 aacgccgaac ctccgataca cgcaaacagg aaaatgaaac catccactga cgctgcacgg
      961 caaaagccca gtctggcgct ggccatcgct accgcgtgcg gtgtcggtta tcttcccaag
     1021 gcgcccggca catttggttc attggtcgga attctcacgg ccatggccac cgcgttgttt
     1081 tttctgcgcc cccatagcct gcgcgatctg ctttccacgc gtcgcctgac cgaatctacc
     1141 ctcatggatc acaacttcct ggtccccgga gccgagatcc acaacgccgc tctggttttg
     1201 ccggtggtct ccgcgatcct cctggttctg ctgctttcct ttgtcggggt atggagcgcc
     1261 ggcaaagcag ccgcttacgc cggtctcaaa gacccgcagc acgtggtaat cgacgaagtc
     1321 gccggccagc acatcacatt gatcctgccg ctcctcccta ttgctgttcc caatctcgcc
     1381 acgcatatgg atttctcgac gtatgcgatt ttttccgcgc tgagcatgct gaactggaaa
     1441 tacctgctcg ccgggtttat ccttttccgc ttgttcgaca tttggaagcc ctatccgatc
     1501 ggcaatctgg agaaactgca gggcggctgg ggaataatgg cggacgactg gctcgcggga
     1561 gtctacgctg ccattccgct gaaggtcgcg ttacactttg gtttgttcgc gtttcatctt
     1621 ggttcatcat gaaatttggt ctgggctcta gcgaaaaaaa cggaattccc cgcatcgaag
     1681 gggtttcgcc ctcggcggcg attcccggcg gcgaagtcga aattcgcggc agcggcttcg
     1741 tcagccgcac gttcgcgcgc cctcaagttc atttcggcga agcggaagcc agcgttgcga
     1801 tgtccgggcc gcagcggctc gtggtgcgcg tcccggaagg cgccagcggc ggcgcagttc
     1861 gcgtagccgc tggcagtcac gaaagccagc ccttccccgt ctcggttggc ctgcagatcg
     1921 ccgacaatct gcatcccgta gcgaaccccg ccgtcgacgc cgaaggcaac acctacgtga
     1981 ccttcagcgg tccgcgcggc cagcgcgtgc ctgtttctct ttacaaaatc accgccaact
     2041 tcaacgtgaa gccgttcgtc acttcgctca ccaatcccag tggactcgcg ctcgatcgcg
     2101 ccggaaatct cttcgtctcc tgcaggaatg acggcaccat tcaccgcgtt tctcccgacg
     2161 gccgcgccga acaatggatt gaaggtatgg gcgtcgccac cggcctgact ttcgatcgcg
     2221 aacaaaatct ctacgtcggc gatcgcagcg gcaccatttt caagattagc ccgtcccgcg
     2281 aaatctttgt cttcgccaca ctcgaacctt ccgtcgccgc ctaccacctg gcatttcatc
     2341 caaccggcga actgtacgcc accggcccca ccacttcgag cttcgatcgt gtctatcgca
     2401 tttcgcaggg tggtgaagtc accacatttt ttcgcggact tggccggccg caaggaattg
     2461 ctttcgatcg agacggcaac ctctacgtcg ccgcgtctct gggcgggcgc cgcggtatcg
     2521 ttcgcatctc ccaaatgggc cgcgcagaac tcgtccttag cggcggtgcc attgtcggaa
     2581 tggccttgct gccctccggc cgcgccatgg tcgccacctc caacgccatt ttcacacttg
     2641 actgggacgt tcgcggcacg ccgctattcg gctagaccaa tttctccgcc ccatcagcct
     2701 tgtacccgag ttgcatagcg ctctcgctct gggtaaaact aaatttcgat gaaagcggaa
     2761 atcattgcgg ttggctcgga gctcctcacg cccgatcgcc aggacaccaa ctcactgttc
     2821 ctgactgccg aattaaacaa gctgggtatc gaggtggtgc gcaaaacgat cgttggcgac
     2881 gagcgcgctc ttctcctgga agcatttcgc gacgccctga accgcgtgcc catcatcatt
     2941 gcgtgcggcg gcctcggtcc cacggaagac gacgtcactc gcgaaactgt cgccgaattg
     3001 ctcggccgca aactggttcg caacgatgcc gtcgtacaat cgattgaagc ccgcttccgt
     3061 tcctttggcc gccagatgac cgaaaacaat ctgcgccagg ccatggtccc ggaaggcgcc
     3121 gaaccgctcg agaatccgcg cggtaccgcg cccggcctgt ggctcgaaaa aggcgaccag
     3181 gccatcgccc tgctgcctgg ccctccccgc gaattgaagc cgctctttct cgagcaagtt
     3241 ctgccgcggc tgcaacgtcg cgctccggct ggaaaaatgt tccaccgcga gttgcgggtc
     3301 accggccttg gcgaatcgca cgtggatcat ctcgccggtc caatctacac gcgttatcct
     3361 gaagtgcaaa ccaccattct tgcgtcgccc ggtgaagtgc agctgcacct ccgtatctgg
     3421 agcggcgaca cacggcacgc cacaagaacc ctggccgatc tcgagcaggc cctggacctc
     3481 gctcttgccg aacgaatttt ttcccgcgat ggctctccgc tcgaagttgt cgtcgcacgc
     3541 gatttactaa aacactgtgc caccatctcc gccgcggaaa gctgcactgg cgggttgctc
     3601 tccgaacgcc tcactaccat cgcgggcagc tcggcttatt ttctcggcag cgtggtttgc
     3661 tacagcaatg atctaaaaac tgcctgggcc gacgttccca ccgaaatgat cgccgcaaaa
     3721 ggagctgtga gtcccgaggt cgcggtggca ctggcgcaag gcattcgccg ccgcgtcggc
     3781 agcacgttgg gcgtcggcat caccggcatc gccggacccg gaggcggcag cgaagaaaaa
     3841 cccgtcggca ccgtacacat tgccgtggcc agcccggccg gcgttcgcga acgcaccgcg
     3901 cgttttcctg gcgatcgcga aatgatccgc atgcaggcct cgcaagccgc cctcgatatg
     3961 gttcgcttgc attttctcta caacggccag gcgccgcccg cgcgttcctg atatgcggct
     4021 ttttgtggcc ctgcagctct ctgtcgaagt gcgcgagaca ttcgccgcgc tgcttggcga
     4081 gctgcggcgc gctgatccca aaccgcgatg ggcagagccc gaaaatttac acatcaccct
     4141 gaaattcatc ggccatattg ccgacgaaga gctgccgctc atctgtcatg cgctcgccgc
     4201 tgtcccggtt ccgccgccaa tgacc
//
LOCUS       DASTPM010000489         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_421177, whole genome shotgun sequence.
ACCESSION   DASTPM010000489 DASTPM010000000
VERSION     DASTPM010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421177"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /gene="sat"
                     /locus_tag="VFR42_08705"
     CDS             complement(<1..1351)
                     /gene="sat"
                     /locus_tag="VFR42_08705"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEU0369275.1"
                     /translation="MQDRQVGTDITLQCRQTRSAALYLTLRETAPNSIEALFMMNIDT
                     AKSHGHLIAPHGGELVDLRLNPESAAEVKARSKDFPSWDLTSRQIRDLELLMSGGFSP
                     LRGFMNRKEYERVCHEMRLSNGMVWPIPITLDVPDAFAKLLTPGSSKIALRDSEGVML
                     AVLYVEDVWQPDLKAEAQAVFGTTSTVHPGVDYLLNRGHHWYVGGRIEGLQLPTHYDF
                     RTLRLTPSDLRAEFARLGWRRIVGFQTRNPMHRAHVELTFRAAAQVEASLLIHPSVGM
                     TRTGDVDYFTRVRCYQLLVSKYPRGTARLALLPLAMRMGGPREAIWHAIIRKNHGCSH
                     FIVGRDHAGPGNDADGKPFYGPYEAQELFRKFEPQIGVEMVPFQMMVYVEDRDKYFPE
                     NEVPTGARVLSLSGTQLRRRLNDGRDIPAWFTFPEIARELRRTFPPRNKQGMTVFFTG
                     "
     gene            1380..1916
                     /gene="nuoB"
                     /locus_tag="VFR42_08710"
     CDS             1380..1916
                     /gene="nuoB"
                     /locus_tag="VFR42_08710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEU0369276.1"
                     /translation="MAETRATAYDSKIEGNVIFTRFDAALNWVRKNSLWPMPMGLACC
                     AIELMATAASRFDISRFGAEVMRFSPRQCDVMIVAGTVTYKMALAVKRIYEQMPEPKW
                     VIAMGACASTGGMYRSYAVLQGIDQLLPVDVYISGCPPRPEALLAGLMKLQDKISRDR
                     AFRDQKPELAEKLEEALA"
BASE COUNT          454 a          616 c          621 g          459 t
ORIGIN      
        1 gaccagtaaa aaacacagtc atgccttgtt tgttccgcgg tgggaaggtg cgccgcagtt
       61 cgcgcgcgat ttcgggaaaa gtgaaccagg ccggaatatc gcgaccgtcg tttaggcgtc
      121 gccgcagttg tgtgccggag agactgagca cgcgcgcgcc agtcggcact tcgttttccg
      181 ggaaatattt atcgcggtcc tcgacataga ccatcatctg gaacggcacc atttccacac
      241 cgatctgggg ttcaaattta cggaacaact cctgtgcttc gtacgggccg tagaatggct
      301 tgccatcagc atcattcccg ggcccggcgt gatcgcgccc gacaatgaaa tgcgagcagc
      361 cgtgattttt tcgaatgatc gcgtgccaga tcgcttcgcg cggtccgccc atccgcatgg
      421 caagaggcaa caacgcgagc ctggctgtgc cccgcggata cttggagaca agcaactgat
      481 agcagcggac gcgcgtgaag tagtccacgt ccccagtccg cgtcatgccg accgacggat
      541 gaatcagcag actggcttcc acttgcgcag cggcgcgaaa agtcaattcc acgtgcgcgc
      601 ggtgcatcgg gttgcgcgtt tggaacccga cgatccgtcg ccagcccagg cgggcgaact
      661 ccgcgcgcag atctgacggt gtaagccgaa gggtccggaa atcgtaatgg gtgggcaatt
      721 gcagaccttc tatgcgcccg cccacgtacc agtggtgtcc tcggttgaga agataatcga
      781 ctccggggtg taccgtgctc gtcgttccga aaacagcttg cgcttcggct ttaagatcag
      841 gttgccagac atcttcgaca tacaacaccg ccagcattac gccttcggaa tcacggagcg
      901 cgatcttgct gcttcccggc gtcagcaact tcgcaaaagc gtccggcaca tcgagggtga
      961 tcggaatcgg ccagaccatg ccgttactca atctcatttc atgacacaca cgttcatact
     1021 ctttccggtt catgaagccg cgcagcggcg agaacccgcc actcataaga agctccaggt
     1081 cgcggatttg ccgactcgtt aagtcccagg atggaaaatc tttggagcgc gctttcactt
     1141 cggcggcgct ttccgggttg agcctaagat cgaccagttc gccaccatgc ggcgcaatga
     1201 gatggccgtg cgattttgca gtgtcgatat tcatcataaa gagcgcttct atactattgg
     1261 gagcggtttc ccgcaacgtg aggtagagcg ccgcgctgcg cgtctgccga cattgcagcg
     1321 tgatgtcggt accaacttgt cgatcttgca cgcgcaattc ttcacgttac gctctggaca
     1381 tggcagaaac acgcgcgacg gcctacgatt cgaagatcga aggtaacgta atattcacgc
     1441 ggttcgatgc ggcgctcaac tgggtgcgga agaattcgct gtggccgatg ccgatggggc
     1501 tggcgtgttg cgctattgaa ttgatggcca cggcggcgtc tcgatttgat atatcccgtt
     1561 ttggcgccga agtgatgcgt ttttcgccgc ggcagtgcga cgtgatgatc gtagccggca
     1621 cggtcaccta caaaatggcc ctcgcagtga aaagaattta tgagcaaatg ccggagccaa
     1681 aatgggtcat cgccatgggc gcgtgtgcgt ccaccggcgg gatgtaccgc tcctacgccg
     1741 ttctacaagg catagatcag cttctgccgg tggacgttta tatctccggc tgcccgccgc
     1801 gtccggaagc gttgctcgcc ggcttgatga aattgcagga caagatttct cgcgaccgcg
     1861 ccttcagaga tcagaaaccc gaactggccg aaaaactcga ggaagcgctg gcatgattga
     1921 ttcgctgtcc tcccgagcga agtcgaggga tcccgtagcg ttaccgctcg gtttccaccg
     1981 caggattctt cgactccgct tcgctccgct caggacgact gcgcgtttcg gctccgtatg
     2041 accgctcaag aactgttcga ttcgctggag agagcgttcg gtgagaaact cgaacgcaaa
     2101 actgaattcc gcggtgaaat aacgtacgcg atcgctccga gcgatatccg
//
LOCUS       DASTPM010000490         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_997812, whole genome shotgun sequence.
ACCESSION   DASTPM010000490 DASTPM010000000
VERSION     DASTPM010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997812"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFR42_08715"
     CDS             <1..528
                     /locus_tag="VFR42_08715"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEU0369277.1"
                     /translation="GKYAGLSSYLAGVSSLDLVTVPHPAIPNLVAIPTGPLPPNPADL
                     LSSHRLPDAIRQLRSEYKFIVIDSPPIMAATDAVIVSVQADGVLLVVRSGETPKEAFT
                     RARDLLTSVKCRLLGVVLNAVDSSAPDYYYSYRYYPYSYGYGPQEAAELYSKGDRPAA
                     SSRAKYTDDEDSQSL"
     gene            547..1551
                     /locus_tag="VFR42_08720"
     CDS             547..1551
                     /locus_tag="VFR42_08720"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU0369278.1"
                     /translation="MRYLITGGAGFIGSHLAERLLDRGDHVVLLDNLSTGSMENIRHL
                     KTSERMEYHLDAIENRQLIAELVDDADVIVHLAAAVGVRLIVESPVRTIETNVNGTQI
                     ILEAAEKKRKLVLTASTSEVYGKSTQVPFREEADLVLGPTTKGRWSYAASKALDEFLA
                     LSYWRERKVPVIVVRLFNTVGPRQTGRYGMVLPNFVKQALDNAPITVYGSGTQSRCFC
                     DVRDTVEALVRLMDTERSVGEVVNIGNTEEISIGDLAQTVRARTGSSSEITYVPYDQA
                     YEPGFEDMMRRVPAVEKLVSLTGFRPQIKLEEIIDRVSRYLAEKKAASRPREVSSIHA
                     "
     gene            1769..3175
                     /locus_tag="VFR42_08725"
     CDS             1769..3175
                     /locus_tag="VFR42_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369279.1"
                     /translation="MVLLVVSLPLVNPWVRGDGVGYYAFARALLIQHNLDFTADYQHA
                     NQGFREARLGSDGSPKEAFRTPTGHLDNHFSVGPAILWAPFLICAHVGVLVARAVGAR
                     TLADGFSLPYLLAMAVGTLLYGFLALLLSYRVACRFVAERWAMLATIAIWWASSLPVY
                     MYFNPSWSHAHSAFAVALFFWYWLRTRDSRTTAQWITLALIAGLALNVYYPNALALAI
                     LVPEGFVAYATAWKKSANAPSLPRVLTRHFLFCFVTLFTLFPTLLTKYIVYGGLFETG
                     YIPISQWNWGSPWFLALLFSANHGLFSWTPILFFATVGLFLFARKFLSVGLSVLCALL
                     VFYYFMAAYPDWPGISSYGNRFFVSLTVFFVLGLAVLLESAASFLRNRAVGSVAIPAI
                     VGVFVLWNLGLIFQWGSHLIPARGPVSWRQVAHNQIHAVPPQLSSQLRSYLFRRKETL
                     HQIEQRDLEQQRRAPVNP"
     gene            3297..4346
                     /locus_tag="VFR42_08730"
     CDS             3297..4346
                     /locus_tag="VFR42_08730"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0369280.1"
                     /translation="MSSNPAPAGIRPPSSPRVAVVSAEGNLSAAVCREAKALSPHIFP
                     QSLPARVLIKPNLCDIVAWENGVTTDPAWLRILASELRSIRPDVKIAVIESDAIGAYK
                     TYRSCYETYQRLGYIDAARNAGAELINLSKAASLDIRVPELPFPMTIPEIFLEEFFFI
                     SVANLKVHPYEHMTGILKNSLGLLPVADISHYHPYLATVISALHRLCPPDLCIIDGRI
                     GLEGKGPIQGDPVRTNTIILSNDALAADLTACRLMTIAPQTVPHLRQTLKDLTRSYDD
                     FAVAGELRPHKFTFDQQQLHPEILAKFRNRRLHRESEIFTNRWISRFFRFKQEPITFL
                     TGAIPKLARRVYGSR"
     gene            4333..>4688
                     /locus_tag="VFR42_08735"
     CDS             4333..>4688
                     /locus_tag="VFR42_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0369281.1"
                     /translation="MALDRLRIGVVGAGPVAEKYHLGAIRGVPEVFAEYVVDVDGQRA
                     EKFATRNGFRRFGASHREMIGAVDIAIVALPNYLHAPVSIDLLSQGIHVLCEKPMART
                     PAECQTMVDAASRSGA"
BASE COUNT          882 a         1434 c         1248 g         1124 t
ORIGIN      
        1 ggaaaatacg cggggctaag ttcgtatctg gccggcgtct ccagcctgga tttggtgacg
       61 gttccgcatc ccgcgattcc gaacctggtc gccataccga ccggcccgtt gccgccgaat
      121 ccggcagatc tgctgtcttc ccacagattg cctgacgcga ttcgccagtt gcggagcgaa
      181 tataaattca ttgtgattga ttcgccgccg atcatggcgg ctaccgatgc ggtgatcgtc
      241 tcagtgcaag ccgatggcgt cttgctggtg gtacgcagcg gggaaacgcc gaaggaagcc
      301 tttacacgcg cgcgcgacct gttgaccagc gtcaaatgcc ggctgctggg cgtggtgctg
      361 aacgcggtgg actccagcgc gcccgattac tactattcgt atcgctacta tccctattct
      421 tatggatatg ggccgcagga agcggcggag ctatactcta agggcgatcg tccggcggca
      481 agttctcgcg ccaaatatac cgacgacgaa gattcccagt cactttaatt ctttgccggg
      541 ggttgcgtgc gttatctgat tacgggcggc gcgggattta tcgggtcgca tcttgccgag
      601 cggttgctgg accgcggcga ccatgtggtt ctgctggata atttgtctac gggcagcatg
      661 gaaaacatcc gccatctgaa aaccagcgag cggatggagt atcacctgga tgctatcgaa
      721 aatcgccagc tcatcgccga gctggtggac gacgcggacg tgatcgtgca cctcgcggcc
      781 gccgtcggcg tccgactcat cgtagaaagt ccagttcgca ccatcgaaac caacgttaac
      841 ggcacacaga tcatcctgga agccgcggaa aagaagcgca aactggtgct gactgcatcc
      901 acctcggagg tctatggcaa gagcacgcaa gtgccgtttc gcgaagaggc cgacttagtt
      961 ctagggccca ccacaaaagg ccgctggagc tacgccgctt cgaaagctct ggacgaattt
     1021 cttgccctgt cctactggcg cgagcgtaag gttcccgtca tcgtggtgcg gctcttcaac
     1081 accgttggac cacgccagac cggccgctac ggtatggtcc ttcccaactt cgtgaagcag
     1141 gcgttggaca acgcacccat caccgtgtac ggatcgggta cgcaatcgcg ctgcttctgc
     1201 gacgtgcgcg acaccgtgga agctctggtg cgtttgatgg ataccgagcg atccgtcggc
     1261 gaagtcgtga acatcggcaa cactgaggaa atttccatcg gtgacctggc tcaaacggtt
     1321 cgcgcccgca ccggcagctc ctccgaaatt acttatgttc cttacgacca ggcctatgag
     1381 cctggttttg aggacatgat gcgccgcgtg cccgccgtcg agaaacttgt atctttgacg
     1441 ggattccgtc cgcaaatcaa actggaagaa atcatcgacc gcgtgtcccg ctacctggca
     1501 gaaaagaagg ccgcatccag gccacgcgag gtttccagca ttcacgcctg agcgcgagcc
     1561 cgatgtcagg cactcagtac acccagctca ttcatttgtt gtcttatact ttgtacacct
     1621 gtaagaaacc aagctggcga tccgggtcaa cggctgggag tttcatttct acagaaaact
     1681 ctccgatggg agcggcttgg ttgtgcaacg tttgaacgac gcaaattccc acgctgccag
     1741 cccgcgaaag ttttcactac taatcgtctt ggttctgctc gtcgtttcgc tacctctggt
     1801 gaatccctgg gtgcgcggcg acggggttgg atactacgcc tttgctcgcg ccttgctgat
     1861 tcaacataac ctggacttca ccgccgacta ccagcacgcc aaccaaggat ttcgcgaagc
     1921 acgcctcggt tcagatggat cacccaagga agccttccgc acgcctacag gccatctgga
     1981 caaccatttt tccgtggggc cagcgattct atgggcgcca tttctgattt gcgcgcatgt
     2041 cggcgtcctc gttgcacggg ctgtgggcgc tcgcacctta gcggatgggt tctcgttgcc
     2101 ttatttgctg gccatggcgg tgggaacgtt gctgtacggc tttttggctc tcctgctctc
     2161 ttatagagtg gcgtgccggt ttgtcgccga acgttgggcg atgctcgcca ccatcgccat
     2221 ctggtgggcc agttcgctac cggtgtatat gtacttcaac ccgtcctggt cgcatgcgca
     2281 ttcggcgttc gccgtggccc tcttcttctg gtattggctg cgcactcgcg attcccgcac
     2341 taccgctcag tggatcactc tcgcgctcat cgcgggactc gcgctcaatg tttactaccc
     2401 caacgcactc gcgctcgcaa tactcgtgcc ggagggtttc gtcgcgtacg caacagcatg
     2461 gaagaagtcc gcgaacgctc cgtcacttcc gcgagtgctc acacgtcatt ttttattttg
     2521 ctttgttacg ttgttcactc tgttcccgac tctccttaca aaatacattg tctacggggg
     2581 cttgttcgaa accggctaca tcccgatctc tcaatggaat tggggctcac cctggtttct
     2641 ggcactgctg ttttccgcga atcacggttt gttctcctgg acgccgatct tgttcttcgc
     2701 caccgtcgga cttttcttgt tcgcgcgcaa gtttctttcc gtcggccttt cggttttgtg
     2761 cgccttgctg gtcttctact acttcatggc tgcctatcct gactggccgg gcatctcctc
     2821 ttacggcaat cggtttttcg tttcgctgac tgtatttttt gtcctgggcc tggcggtcct
     2881 tcttgagtcc gcagcgtcct tcttgcgcaa tcgagccgtt ggttccgtag cgattccggc
     2941 aatcgtgggc gttttcgttc tgtggaatct cggccttatc ttccagtggg gctctcactt
     3001 gatccccgct cgcggcccgg tttcctggcg acaggtcgcc cacaatcaga ttcatgcggt
     3061 accgccgcag ctctcatcgc agcttcgttc ttaccttttt cgccgcaagg aaaccctcca
     3121 ccagattgaa cagcgtgacc tcgagcagca gcgtcgtgcg cccgtgaacc cctgaaccac
     3181 tactgcggtg atctttccgg cgagcgaatt tctgatattc tttgtttgca attgcgagcc
     3241 tgatctgctc ctcggccctc ccagcagcaa gggcatcaca gtggagttcc attttcatga
     3301 gttccaatcc agcgcctgcg ggaattcgtc cccccagctc tccccgggtt gccgtcgtat
     3361 ctgcggaagg aaacttatcg gccgcagtgt gccgcgaagc gaaagcgctt agcccgcaca
     3421 ttttcccgca gtctctcccg gcccgagtac tgatcaagcc aaacttgtgc gacatcgtgg
     3481 cctgggaaaa cggtgtgacc accgaccctg cgtggctgcg aatactcgct tccgagctcc
     3541 gttccatccg accggatgtg aaaatcgccg tcatcgaatc ggacgctatc ggcgcctaca
     3601 agacgtatcg ttcctgttac gaaacctacc aacgtctggg ctacattgat gcggcgcgca
     3661 acgcaggcgc cgaactgatc aatctctcca aagctgcgtc actcgatatt cgtgtgccgg
     3721 aactgccgtt tccgatgacc attccggaaa tttttttgga agaattcttt ttcatctcgg
     3781 tggccaacct caaagttcat ccttacgagc acatgaccgg catcctcaag aactctctcg
     3841 gattactgcc ggtggcggac atctcgcact atcaccccta tctcgccacc gtgattagcg
     3901 cgctccacag gctctgtcca ccggacctct gcatcattga cggccggatc ggccttgagg
     3961 gcaagggccc catccagggc gatcccgtcc gcaccaacac gatcatcctc agcaacgatg
     4021 cgcttgccgc agacctcact gcctgccgtt tgatgaccat cgcgccgcaa acggtcccgc
     4081 acttgcgcca gacattaaaa gacctgaccc gcagctacga cgactttgct gttgccggcg
     4141 aactgcgtcc ccacaaattt acgtttgatc agcaacaact gcatcctgag atcctcgcca
     4201 agttccgcaa ccgacggctg catcgcgaat cggaaatttt caccaatcgc tggatcagcc
     4261 gtttcttccg ctttaagcag gagccgatta cgttcctgac cggtgccatt cccaaattgg
     4321 cgaggcgtgt atatggctct cgataggcta cgtatcggcg tggttggtgc cggcccggtc
     4381 gccgaaaaat atcatcttgg cgcgattcgc ggagtccccg aagtatttgc cgaatacgtt
     4441 gtggacgtcg atggccaacg cgcggaaaaa ttcgcaacgc ggaacggttt tcgccgcttc
     4501 ggagcatccc atcgggagat gatcggcgcg gtcgacattg ccatcgtcgc gcttccgaac
     4561 tacctgcatg ctcccgtttc gattgacttg ctctcgcaag gcattcacgt tctttgcgag
     4621 aagcccatgg ctcgcactcc ggcggaatgt caaaccatgg tcgatgccgc cagtcggtcc
     4681 ggcgcgtt
//
LOCUS       DASTPM010000491         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_882475, whole genome shotgun sequence.
ACCESSION   DASTPM010000491 DASTPM010000000
VERSION     DASTPM010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882475"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFR42_08740"
     CDS             <1..249
                     /locus_tag="VFR42_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU0369282.1"
                     /translation="EVTREPYPDPHDREGKNLVADLRAFEKLPRQVTLTELRSNKGLK
                     KVKFLKNVRLIISPITDIEYQEILRMAGIVATPGIPLP"
     gene            294..1100
                     /locus_tag="VFR42_08745"
     CDS             294..1100
                     /locus_tag="VFR42_08745"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0369283.1"
                     /translation="MAKLARVLFGSAVFLAATTIFLGQTSTHKPALEEIASGKTRVID
                     LSYAISDKLVPWPGDSKAFEAKTNATVEKEGYFTRSFWMLEHYGTHMDAPAHFPPGTT
                     TVDKIAPEKFVGPAVVLDVTKEAERDPDYQLSAKDVFTWEQKHGNIPAGAIVILRTGW
                     SARWPDVARYRNQDVNGKMHFPGFSVEAAKILLQRKVSGLGCDTLSIDTGNSPDFPVH
                     HLVLGAGVYQLENLADLSGLPEAGAFLIAAPIKLEGGSGGAVRVFALLGS"
     gene            1174..2535
                     /locus_tag="VFR42_08750"
     CDS             1174..2535
                     /locus_tag="VFR42_08750"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0369284.1"
                     /translation="MNPHKILVRVLLCGALMIACASGMFAQNEAAQFPIATKTLKNGM
                     KVLVQTDHSIPNVALYIFYRIGSRNEHPGTTGLSHFFEHMMFNGAKKFGPGELDKAME
                     ANGAANNAYTNENTTVYQDWFPRSALQLIFDIEADRIQYLSFDPKRVESERGVVASER
                     RTEVDASNSGILDEQLQATAFVAHPYQWPVVGWMSDIEHWTMDDLKHHFEMGYAPNNA
                     TMVVVGDVTPEEIFQLCEKYIEPIPQHAPPPNVTTVEPEQMGERRLVVRKAAELPLLQ
                     IAYHVPQTNNPDFYALNILRTVLVQGESSRMYQRLIDKDQIALDVGISLSPAFDPTIL
                     KMSIQPKQGVDPQKCEAAVYEELDKVKKGSISDQELEKAKNIRLVEFYQQMRTINGRA
                     NTIGTYEVFFSDYHKLFDAAKNYRAVTKEDVQRVAQKYFGENNRTVATLIPDHAQEGS
                     AKQ"
     gene            2553..>3048
                     /locus_tag="VFR42_08755"
     CDS             2553..>3048
                     /locus_tag="VFR42_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU0369285.1"
                     /translation="MRCLTAMSLALVLVVMAEGQTLKLPPHEKITLKNGMTVLLMEKR
                     GVPMVSFAAIVKAGAAADPVGLEGLASSTAGLLRKGTTKRNAQKFAEDLDFIGASFSA
                     DASADFTALSAECLSKDLDKGLDLLSDAMLHPAFPQGEVDKLLSQDIDGVKAAKDEAE
                     SVALT"
BASE COUNT          724 a          792 c          946 g          586 t
ORIGIN      
        1 gaagtcaccc gcgaacccta tcccgacccg cacgaccgcg agggcaagaa cctggtggcg
       61 gacctgcgcg catttgaaaa gctgccgcgc caggtgacac tcaccgagct gcgcagcaac
      121 aaggggctga agaaagtaaa gttcctcaag aatgtgcggc tgattatttc gccgatcacc
      181 gatatcgagt accaggaaat tctgcgcatg gccggaatcg tggccactcc aggaattccg
      241 ctgccgtaag ttggcgccga cacttttccc caattgccat gagaggtgct gttatggcga
      301 agctcgctcg agtattgttc ggctcggccg tgttcctggc ggcaaccaca atctttcttg
      361 gacagacttc cacacacaag cccgcgctgg aggaaatcgc cagcggcaag acgcgcgtga
      421 tcgatttgag ctatgcgatc agcgacaaat tggtgccgtg gccgggcgac tccaaggctt
      481 tcgaagcgaa aaccaacgcc acggttgaga aggaaggcta cttcacgcgt agtttctgga
      541 tgctggagca ctacggaacg cacatggatg cgcccgcaca cttcccgccg ggaacaacga
      601 ccgtggacaa aatcgcgccg gagaaattcg ttgggccagc ggtggtgctc gatgtcacga
      661 aggaggccga gcgcgaccca gattaccaac tcagcgcgaa ggacgttttc acttgggagc
      721 agaagcacgg aaatattccg gcgggcgcaa tcgtaattct caggaccggc tggtccgcgc
      781 gatggccgga cgtggcgcgg taccgcaacc aagatgttaa cggcaagatg catttcccgg
      841 gtttttcggt ggaggcggcg aagattctgt tgcagcgaaa agtgagcggc ctgggttgcg
      901 acacactgag cattgatacg ggcaactcgc cggattttcc tgtgcaccat ctggtgctgg
      961 gcgcgggagt ctatcagctg gaaaacctgg cggatttgag cggcttgccg gaagcgggcg
     1021 cgttcttgat tgcagcgcca atcaagctgg aaggtggctc gggcggcgcg gtgcgggtgt
     1081 tcgcgctgct agggagctaa atcacggctt gcactagaac attttcacgg tttcatacgt
     1141 ctgattagct gggatgtccc ctgggggtgg ttcgtgaatc cacacaagat tcttgtgcgc
     1201 gttttgcttt gcggggcgtt gatgattgcg tgcgccagcg ggatgttcgc ccagaatgaa
     1261 gccgcgcagt tccccatagc cactaagacc ctgaaaaacg gcatgaaagt gctggtgcag
     1321 acggaccaca gcataccgaa cgtcgcgttg tatattttct atcgaatcgg atcgcgcaat
     1381 gagcatcccg gaacgacggg cctttcgcac tttttcgaac acatgatgtt caatggcgca
     1441 aagaagttcg gacccggcga actggacaag gcgatggaag ccaacggggc cgcgaacaac
     1501 gcctatacca acgaaaacac cactgtctat caggactggt ttccccgttc ggcgctgcaa
     1561 ctgattttcg acatcgaagc ggaccgtatt caatatttga gtttcgatcc caagagagtc
     1621 gaatcggagc gcggtgtggt ggcgtcggag cggcggacgg aagtggacgc gagcaacagc
     1681 ggcattcttg acgaacagct gcaggcgacg gcgttcgtcg cccacccgta tcagtggccg
     1741 gtggtcggct ggatgagcga catcgaacac tggaccatgg acgatttgaa acaccatttt
     1801 gaaatgggtt acgcgccgaa caacgcgacg atggtggtgg tcggcgacgt cacaccggaa
     1861 gagatttttc agctctgcga aaaatatatc gagccgattc cgcagcacgc gccgcctccg
     1921 aacgtgacta ccgtagagcc ggaacagatg ggtgagcgca ggctggtggt gcggaaggca
     1981 gcggagttgc cgctgctgca gatcgcctac cacgtgccgc aaacgaacaa ccccgacttt
     2041 tatgcgctga acattttgcg caccgtgctg gtgcagggcg agagctcgcg gatgtaccaa
     2101 cggttgattg ataaggatca gattgcgctg gatgtcggca tatcgttaag ccccgcgttt
     2161 gatccgacga ttctgaagat gtcgatccag ccgaagcaag gagtggaccc gcagaaatgc
     2221 gaagcggcag tttacgagga gctggacaaa gtgaagaagg gttcgatcag cgaccaggaa
     2281 ctggaaaaag caaaaaacat ccggctggtg gagttttacc agcagatgag aacgatcaac
     2341 gggcgggcca acaccatcgg cacctacgaa gtatttttta gcgactacca caagttattt
     2401 gacgcggcga aaaactatcg cgccgtgaca aaggaagatg tgcagcgggt ggcgcagaaa
     2461 tactttggcg aaaataaccg aaccgtcgca acgctgattc ctgatcatgc ccaagagggg
     2521 agcgccaagc aatgaaccgg cgacggacca cgatgcgctg tctgaccgcg atgtctctgg
     2581 cactggtgtt ggtggtgatg gcagaagggc agacgctgaa gctgcctcca catgagaaaa
     2641 ttacgttgaa aaacggcatg accgtgctgc tgatggaaaa gcgcggagtg ccgatggtga
     2701 gttttgcggc gatcgtgaaa gcaggcgcgg cagctgatcc cgtgggtctg gagggactgg
     2761 catccagcac cgcgggactg ttgcgcaagg gcacgaccaa acgcaacgca caaaaattcg
     2821 cagaagacct ggatttcatc ggcgcgagtt ttagcgctga tgcgagcgcg gactttaccg
     2881 ccctttccgc cgagtgcctg agcaaagacc tggacaaggg attggatttg ctgagcgacg
     2941 cgatgctgca tcccgcgttt ccgcaaggcg aagtcgacaa gctattatcg caggatattg
     3001 atggagtgaa agcggccaag gatgaggcgg agtccgtcgc gttgacct
//
LOCUS       DASTPM010000492         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_621577, whole genome shotgun sequence.
ACCESSION   DASTPM010000492 DASTPM010000000
VERSION     DASTPM010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621577"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VFR42_08760"
     CDS             <1..1164
                     /locus_tag="VFR42_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0369286.1"
                     /translation="GAMIQLPDAKSLLKYLRPKLPAMLDTLKEFVCCESPSTEKAAAD
                     ACASLIAATWKRRRVQVEVLEQKHRGAHLRITHVPDGGNTCGQVLLLGHYDTVYASGT
                     LQKMPFRIAGGKIFGPGAFDMKAGIVQALFALDALQKLAAPLAKRIVFLWTSDEEIGS
                     ESSRKLLEAEARRSDAVFVLEPALGPQGLLKTARKAVGDAEIIVHGRASHAGLAPEKG
                     VNAIHELALQLARVKQWNNLTRGISVNAGVIEGGTRSNVIPDRARAVVDLRALRIADM
                     RAIEKRLRALKPVSPGAKLEIRGGFSRAPLERKHSAALFQRAAMLAKQMGLKIGECTA
                     GGGSDGNLTAALGIPTLDGFGAVGDGAHSPGEHIIAKTMPERAALLAAMLMHS"
     gene            complement(1238..1564)
                     /locus_tag="VFR42_08765"
     CDS             complement(1238..1564)
                     /locus_tag="VFR42_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369287.1"
                     /translation="MIKPFGLYWPRDIENLEEIDDVAGPKSGFYVLYHGAMQLYIGRG
                     AISARLLSHAKEGSKKEQLSGRFSWFVVGNSKQERELESLPLEALPFYVRSLNQQTGK
                     LSRKCR"
     gene            complement(1604..3064)
                     /locus_tag="VFR42_08770"
     CDS             complement(1604..3064)
                     /locus_tag="VFR42_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369288.1"
                     /translation="MRWTSKTGCGWVLLCVLMAAPAAFAQGNTIVLKNGRRIVALTVT
                     QDGDKVRYETSAGELTLPRAIVDHIESGGMPTLGDSADAGKLSLKPPEAATADSALTG
                     RTEIEARVIHDGEVDRNYVAKLENEALSGQSLANWNAALAHHTASQFELAHGEMDLAL
                     ADEQQALKYAPQQPVFLMNAAYLHLRRSEYRQSLEYLERARRVAPEDPDVAKLTGWAY
                     YGLNKMDQAVAEWQHALALRPDAETEAALKKALRDKEEEAHYKENESRHFTLRYSGAA
                     EPAMARDVLRTLEHHFDAIESELSYSPPDAVGVILYTQQAFADITQAPAWVGALNDGR
                     IRVPVQGLSSVTPELSRVLRHELTHSFIQQKTRGRAPTWIQEGLAQWMEGKRSDANSP
                     ALIRMAASTPEQAAAHFEGDWMKMSKEVVGLAYAWALANVECILHAGGMTDLDRIMSR
                     LASGEAPEAATKAVTRSDYAELTSDTVDYLSRTYGK"
     gene            3143..>3560
                     /locus_tag="VFR42_08775"
     CDS             3143..>3560
                     /locus_tag="VFR42_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369289.1"
                     /translation="MKSRALLFVIFAAACSTCAVAQEPSPREILSALNRVQADPSAVY
                     KIERGSGIELRRGDAKLQFEDGMLALFAPLDGKITGAVYSGRGHILASPRDPVEKQQL
                     GYFLGAPMVDQDFSNAYLRFTSDTAAELLHQLEVAQI"
BASE COUNT          665 a         1191 c          948 g          756 t
ORIGIN      
        1 ggcgccatga ttcaactccc cgatgccaag agcttgctga agtatttgcg cccgaagctt
       61 cccgcgatgc tggacacgct gaaggaattt gtctgttgcg aatcgcccag caccgaaaaa
      121 gccgccgcgg acgcttgcgc ctccttgatc gccgccacgt ggaagcgtcg cagagtccag
      181 gttgaggttc tcgaacaaaa gcatcgcggc gcacacttgc gcatcaccca tgtacctgat
      241 ggcggcaaca catgcggcca agtccttctg ctgggccact acgacacggt ctacgccagc
      301 ggcacattgc agaagatgcc gtttcgcatc gcgggtggaa aaatcttcgg tccgggagcg
      361 ttcgatatga aagcgggcat tgtccaggct ctgtttgctc tggatgcgct gcaaaaactc
      421 gccgcgcctc tggccaagag aatcgtcttc ctgtggacct ccgacgaaga aatcgggagc
      481 gaatcgtcac gtaagcttct ggaggccgaa gcgcggcgca gcgacgcggt gttcgtgctg
      541 gaaccagccc tcggccccca gggtttgctc aagactgcgc gcaaggcagt cggcgacgcg
      601 gaaatcattg tgcacggtcg cgcttcgcac gcgggactcg ctccggaaaa aggcgtgaac
      661 gcgattcatg aactggctct gcaactcgcc cgcgtaaaac agtggaacaa cctcacgcgc
      721 ggcatctccg tcaacgctgg cgtcattgaa ggcggtaccc gctccaacgt cattccggac
      781 cgcgccagag cggtcgtgga cctgcgcgcc ctccgcattg ccgacatgcg cgccatcgaa
      841 aaacgcctgc gcgctctgaa accagtttcc ccgggcgcaa agctcgaaat tcgtggaggc
      901 ttcagccgcg cccctctcga gcgcaagcac agtgccgcgc tttttcagcg cgccgcaatg
      961 ttggcgaaac aaatgggctt gaagattggc gaatgcacgg ccggtggtgg ctccgacggc
     1021 aatttgaccg cggctctcgg tattccgact ttagatggtt ttggtgcagt cggcgacggc
     1081 gcacactcac ccggcgaaca catcattgca aagaccatgc cggaacgcgc cgcccttctc
     1141 gcggcaatgt tgatgcacag ctgatgtcac gcgccgtcca cagggggcag cagccaagcg
     1201 cggcagcgca acatcattgc ttcgccgtct ggcgccccta cctgcatttg cgagacaact
     1261 ttcccgtctg ctggttcaga ctgcgtacat aaaacggcag cgcttccagc ggcagcgact
     1321 ccagttcgcg ttcttgcttc gaattcccca ccacaaacca cgaaaaccgg ccgctaagct
     1381 gctccttctt cgaaccttcc ttcgcatggc tcagcaatcg ggcgctgatc gctccgcgcc
     1441 cgatgtacag ctgcatcgct ccgtgataca gcacataaaa tccggacttg ggccccgcaa
     1501 catcgtcaat ctcttccaga ttctcgatat cacgtggcca atacagccca aacggtttga
     1561 tcaaccccct gcttccatcg tgctacctca aaattcacaa aatttatttg ccgtacgttc
     1621 tgcttaggta gtccacggtg tcgctggtca gctctgcgta gtcgctccgt gtcaccgctt
     1681 tcgtagcggc ttccggcgct tcgcctgagg caagacgact catgatccga tccagatccg
     1741 tcatccctcc ggcgtgcaga atgcattcca cattcgccag cgcccaagcg taggccaggc
     1801 ctacaacctc tttcgacatt ttcatccaat cgccctcgaa gtgcgctgcc gcctgctcgg
     1861 gcgtgcttgc cgccattcga atcagcgctg gcgaattcgc gtcgctgcgc ttcccttcca
     1921 tccactgcgc taagccttcc tgaatccaag taggcgcgcg tccgcgcgtt ttttgttgga
     1981 taaagctgtg cgtcagctcg tgccgcagca cgcgcgacaa ctccggagtc acactactca
     2041 acccctgcac cggtacgcga atccgtccgt cgttcagcgc gcccacccaa gccggtgcct
     2101 gcgtgatgtc cgcaaatgcc tgctgcgtgt acaaaatcac tccgaccgca tccggcggcg
     2161 aataactcag ctcggattca atcgcgtcga agtgatgctc cagcgtgcgc aaaacgtcgc
     2221 gcgccatcgc cggttccgcg gcgccgctgt agcgcagcgt aaagtgccgg ctttcattct
     2281 ccttgtagtg cgcctcttcc tctttgtccc gcagcgcctt tttcagcgcc gcctccgtct
     2341 ccgcatccgg ccgcaaagcc agtgcgtgct gccattcggc gacggcttga tccatcttgt
     2401 tcaatccgta gtacgcccag cccgtcaatt tcgcgacgtc gggatcttcc ggcgcgaccc
     2461 ggcgcgcccg ctcaagatat tccagcgatt gcctgtactc actccggcgc aagtgaagat
     2521 aggccgcgtt catcagaaag accggctgct gcggcgcgta cttcagcgct tgctgttcgt
     2581 ccgccagcgc caggtccatc tcgccatgag ccaattcgaa ttgcgaggcc gtatggtgcg
     2641 ccagtgcggc gttccagttc gccagcgact gcccagagag cgcctcgttc tccagcttag
     2701 ctacgtaatt ccgatccact tcgccgtcat gaattacccg tgcttcgatc tctgtcctgc
     2761 ctgtcagagc cgaatctgcc gtcgcggctt ccggcggttt taacgacaat tttccggcgt
     2821 cggctgagtc gcccagcgta ggcatcccgc cgctttcgat gtgatccaca atcgcgcggg
     2881 gcagtgtgag ctcgccggcg gaagtctcat agcgaacttt gtcgccgtct tgcgtcaccg
     2941 tgagcgcgac gatgcgcctt ccgtttttca gaacgatcgt atttccttgc gcgaatgcgg
     3001 ccggcgctgc catcaacacg cacagcaaca cccatccgca cccggttttg ctcgtccacc
     3061 gcattttctc aatgtactcc cgcgcgcaaa tttgcttcgg gcaaccttgc tgctgcatgt
     3121 cctatctgac tacactgcca gcatgaaatc ccgggccctc ctcttcgtca tttttgcggc
     3181 ggcgtgttcg acctgtgctg tcgcgcagga accttcgccg cgcgaaatcc tctccgcgct
     3241 caaccgcgtt caagctgacc cttccgccgt atacaaaatc gagcgcggtt ccggtatcga
     3301 gcttcggcgg ggcgatgcca aactccaatt cgaagacggc atgttggccc tttttgcgcc
     3361 actcgacggg aaaatcacgg gtgcggttta ttccggccgt ggccacattc tggcctcgcc
     3421 gcgtgacccc gtcgaaaaac agcagctcgg ctattttctg ggcgcgccca tggtcgatca
     3481 ggacttcagc aatgcctact tgcgcttcac gtccgacacc gccgccgaac tcctgcatca
     3541 gctcgaggtc gcgcaaatca
//
LOCUS       DASTPM010000493         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_392595, whole genome shotgun sequence.
ACCESSION   DASTPM010000493 DASTPM010000000
VERSION     DASTPM010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392595"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFR42_08780"
     CDS             complement(<1..726)
                     /locus_tag="VFR42_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEU0369290.1"
                     /translation="MDRRAFVKRSCLGGFALSLDHWGTSPAFGQDLIPPVPPIHVSEP
                     LGTQVSPLRMPGVYPGRVVEISDARSIVANRVSQAVVGEMLERGLCELTGEKDLRQAW
                     SKFIQPQDLVGIKINPSGAPACCSSPEIVREIVAGVQSAGVPARNIFVYDRYAYEMDI
                     GSYQALVPKGVRVTGVQEHKLDASGYHPNIFCEANFFGEWETRSYMANIVASGVTKII
                     NVPTMKDHSAAGVTGCLKNLGYGT"
     gene            complement(874..2094)
                     /locus_tag="VFR42_08785"
     CDS             complement(874..2094)
                     /locus_tag="VFR42_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0369291.1"
                     /translation="MTSSSMFDVVTVGGGMGASALAIAMARSGARVLVLEKETKFRDR
                     VRGEALTPWGVAEARKLGVADLLLNTCAKEVPWVEMGFGPRNLVETTPQREPFYSYSH
                     PEMQEVLLAEAGRAGAEVRRGVTVEAVEPRGNLSATGEGMYATVTARNGETEKVSARL
                     VVGVDGRGSAVRRWGGFAVEKSPLAFQFAGVQLTGVRWREDLATFIFNPEVGMVLGMV
                     PQTKGRWRSYLGYPRESGLALQGDDKLKTFLAEAARAVPIMAAAYANVESIGPLASFD
                     VSESWAPHPFREGIALIGDAAGTSDPSFGQGMGTALRDARVLRDALLRNPDWDNAGHE
                     YAQEHDTYFQNCHKVCGWLRTLFQDPSAEAGALRHKAMPKITEDLMRVPDHLFSGPDL
                     PADEAVRARFFGEC"
BASE COUNT          459 a          804 c          598 g          474 t
ORIGIN      
        1 cgttccatag cccaggttct tcaagcaacc cgtgacaccc gccgcggaat gatccttcat
       61 cgtcggcaca ttaatgattt tggtcacgcc cgaagccacg atgttcgcca tgtacgagcg
      121 cgtttcccac tccccgaaaa agttggcttc gcagaaaatg tttgggtgat atcccgacgc
      181 atccagcttg tgttcctgga caccggttac tcgaactccc ttaggcacca gcgcttgata
      241 gctgccgata tccatctcgt atgcatagcg gtcataaaca aagatattgc gggccggtac
      301 accagctgac tgcactccag cgacgatttc ccgcacaatt tccggcgaag aacaacaggc
      361 cggagcgccc gaaggattaa ttttgatgcc gaccaggtcc tgcggctgga tgaatttgct
      421 ccaggcttgg cgcagatcct tttcccccgt caattcacag aggcctcgtt caagcatttc
      481 accaaccacg gcttgcgaaa cccgattcgc tacgatggaa cgggcgtccg aaatttccac
      541 cacccggcca gggtacacgc cgggcatccg tagtggcgaa acctgcgtcc ccagcggttc
      601 gcttacatgg attggcggca cgggagggat taagtcctgg ccgaaagctg gagaagttcc
      661 ccaatggtca agagacagcg caaagccgcc caaacaactt cgctttacga atgctcgtcg
      721 atccacggcg cgcgtttcct attgagagct tctgctccag tcgcgccgaa ttctatcaga
      781 gcggcatgat ttttaacgtt gccaaggctc acgccaggcc gacgcccgcc gttccggccg
      841 agagaccacg gtttcccgcg ggcgcgtttc ttctcagcat tcgccaaaga accgggcgcg
      901 aacggcttcg tccgcgggca agtccgggcc actgaagaga tggtccggca cgcgcatcag
      961 atcctcggtg atcttcggca tcgctttgtg gcgcagcgct cccgcctctg cgctcggatc
     1021 ctgaaacagg gtgcgcagcc atccgcacac cttgtggcaa ttctggaaat atgtgtcatg
     1081 ttcctgcgcg tattcgtgcc cagcgttatc ccagtctggg ttgcgcagca gagcatcgcg
     1141 cagtacgcga gcatcccgca gggctgtacc catcccttga ccgaaagaag gatcgctcgt
     1201 tcccgccgca tctccgatta gcgcaatacc ctcgcgaaat ggatgcggtg cccaggattc
     1261 gctcacatcg aaactcgcca gcggcccgat gctttcgacg ttcgcgtatg ctgcggccat
     1321 gatcggcacc gctcgcgccg cctccgctaa gaacgttttc agtttgtcgt cgccctgcaa
     1381 cgccagcccg ctttcgcgcg gatagccgag ataagagcgc catctccctt tggtctgcgg
     1441 caccattccc aagaccatgc ccacttccgg attaaaaatg aacgtcgcca gatcttcccg
     1501 ccagcgcacc cccgtcaact gaactcccgc aaactgaaag gccagcggcg atttttccac
     1561 cgcgaatccg ccccagcggc gcacggctga tccgcggcca tcgactccca ccaccagccg
     1621 cgccgagact ttctctgtct ctccattccg cgcagttacg gttgcataca tcccttcgcc
     1681 cgttgccgat agattcccgc gcggctcgac tgcttccact gtcacgccgc ggcgcacctc
     1741 cgcacccgcg cgccccgctt ccgctagcaa cacttcctgc atctccggat gggagtagga
     1801 ataaaacggc tccctttgcg gcgtcgtctc cacgagattg cgcggaccga atcccatctc
     1861 cacccatggc acttctttcg cgcaagtgtt gagcagcaga tctgcaaccc ccaatttccg
     1921 cgcttccgcc actccccagg gagtcagcgc ctcaccgcgc acgcgatcgc ggaattttgt
     1981 ttccttctcc agcacgagaa cgcgcgcgcc gcttcgcgcc atcgcgatgg caagcgccga
     2041 cgcacccatc ccaccgccca ccgtcaccac atcgaacata cttgaacttg tcatcgcctc
     2101 acctgaccag gtcgttcgat cagttgttcc gaggacttca gaaccgtttc cgcgatgcta
     2161 ccaaattccg cgggctggac gcgcaaataa cttagcccgg ggtgaccacg ctgggaacct
     2221 cggtcgggag attcaagggc cataggtgcg gcaaattccg caaccgccta gggcgcttac
     2281 tgctccggcg tttccccgcc cgtcgcgccg aatccccagc gttcgcgcag ccgtt
//
LOCUS       DASTPM010000494         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_219908, whole genome shotgun sequence.
ACCESSION   DASTPM010000494 DASTPM010000000
VERSION     DASTPM010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219908"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="VFR42_08790"
     CDS             <1..1516
                     /locus_tag="VFR42_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369292.1"
                     /translation="MIRNGEISMSEVKRVLRKYWWILPLTVVSGGALGLGATMVLPKK
                     FTSQSRVQIHEQTVSTEILKPVLTDATNQRLVGMQEKILSRTQLQAIVEKFGLYESER
                     GKLHMEDLVLKLRSAIEVTPPETMLGVQSRQLPGFYINVTFDNPEVAQRICSEITTKF
                     MEQNAKIINDGGVRTTKFLADQAEEAKRNLDEQDAKLAEFKKKYMGSLPDQQGANLSL
                     LQTLDSQLEANTQAISRAQQDKAMNESLLAAQLGVWKATRTGGTPTETLEEQLTTLQE
                     QLSALQSRYTPEHPDVIKTKSQIEQLKKRIADAPKTNQTTTSAAAGEIEPAVIQQLRA
                     KLKQDDLNIADLTKRQSQIQSQIGVMQGRLQLSPAVEQQFKELMRGYQSASDSYNELL
                     KKRDQASIATDYNRQQEGEQFSMEDPPSLPMTPSFPKKPIFAGGGLAGGLALGFAVLY
                     LLAALDSSMHSERDVEVCLKLPVLALVPTMDPTRAAPSNGKGRIKELDFAGTRN"
     gene            1550..2485
                     /locus_tag="VFR42_08795"
     CDS             1550..2485
                     /locus_tag="VFR42_08795"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEU0369293.1"
                     /translation="MSRIHEALKKAAQERSAQLAKGGDTAFVELATAPTAPMSAATAA
                     TMPVREMEEAMAPTAAAPDTLTYLRYEDLVKKCATPQWRIDGRLSVFEGNDDTQLGAE
                     RFRTLRSRLYQIAGTRPLRRVMVTSSVPAEGKTFVAANLAQSIVRQPDRKVLLIDADL
                     RASRLHQVLGAPRSPGLSDYLIGEADEFRVVQKGSDANLCFIPGGREVPNPSELLMND
                     RLKKLLELMTPLFDWIILDTPPVLPVHDASMMADVCDGVLFVVRAGMTDHEMAAKATA
                     EFQDRNLLGVVLNRVEDGAGYGGYYYGYPAGGEKD"
     gene            complement(2482..>2872)
                     /locus_tag="VFR42_08800"
     CDS             complement(2482..>2872)
                     /locus_tag="VFR42_08800"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HEU0369294.1"
                     /translation="WSGVPVLRDGFVFHLARVNIEVARECSGIRSSLALIILAILVAH
                     FYLRTFWRQVVLVLAGLFVMILKNAIRIVTLTLLASYVDPGFLYGNLHREGGVVFFLI
                     GLAILAPLLWWLERGERKPPAVPAGTA"
BASE COUNT          735 a          736 c          918 g          483 t
ORIGIN      
        1 gatgattcga aacggcgaaa tcagcatgtc agaggtcaag agggtcctga ggaaatattg
       61 gtggatcttg ccgttgaccg tggtgagcgg gggcgcgttg gggctgggcg ccacgatggt
      121 gctgccgaag aaattcacat cacagtcgcg agtgcagatt cacgaacaga cggtatcgac
      181 ggaaattctt aagccggtct taacagacgc caccaatcag cgacttgtag gaatgcaaga
      241 aaagatccta agccgcacgc agctgcaggc aatcgtagaa aagttcggtt tgtatgagtc
      301 agagcgcgga aagttgcaca tggaagatct ggtgctgaag ctgcggtcag cgattgaggt
      361 aaccccgccg gaaaccatgc tgggggtaca gagccggcag ctgcccggtt tctacatcaa
      421 tgtgaccttt gacaatccgg aagtcgcgca gcgaatttgc agcgaaatca cgacgaagtt
      481 catggagcag aacgcaaaaa tcatcaatga cgggggagtt cgaacaacca agtttctggc
      541 cgaccaagcg gaggaggcaa agcgtaacct ggacgagcag gacgccaagc tggcggagtt
      601 taagaagaaa tatatgggct cgctgccgga ccagcaaggg gcaaacctca gcttactgca
      661 aacgttggat tcacaactgg aagctaacac ccaagcgatt agccgggcac aacaggacaa
      721 agcgatgaac gagtcgctgt tggcggcgca gctgggagtg tggaaggcca ccaggaccgg
      781 tggcacgcct accgagactc ttgaggaaca gctgaccacg ttgcaggagc agttgtcggc
      841 gctgcaatcg cgctacacgc cggagcatcc cgacgtgatc aagaccaaga gtcagatcga
      901 gcaattgaag aaacggattg cagacgcgcc caaaacaaat cagacgacaa cgagcgcggc
      961 tgcaggcgaa attgaaccgg cggtgatcca gcaactgcgg gcgaagctga aacaggatga
     1021 tctgaacatc gcggacctga ccaaacgaca aagccaaatt cagtcacaga tcggcgtgat
     1081 gcaggggagg ttgcagctga gcccggcggt cgaacagcaa ttcaaagaac tgatgcgcgg
     1141 ctaccaaagc gccagcgact cgtataacga acttttgaag aagcgcgacc aggcatcgat
     1201 tgcgacggac tataaccggc agcaagaagg cgagcagttc agcatggaag acccgcccag
     1261 cttgccgatg acgccgtcgt ttcccaagaa gccgattttt gctggtgggg gattggcggg
     1321 cggattggcg ctgggatttg ccgtgttgta cctgctggcg gcgcttgatt cctcaatgca
     1381 ctcggagcgg gatgtggagg tttgcttgaa gctgccggtg ctggcgctgg tgcccacgat
     1441 ggatccaacc cgggcagccc cgagcaacgg caagggcagg ataaaggaat tggactttgc
     1501 aggcacgcgg aactaaagac ggaaagcgga taaggaacaa gagacatcca tgagccgaat
     1561 tcatgaggca ctgaagaaag cggcgcaaga gcgctccgcg cagctggcaa agggcggaga
     1621 cactgccttt gtggaactgg cgaccgcgcc aaccgcgccg atgagcgctg caacagctgc
     1681 gaccatgccc gtgcgagaga tggaagaagc gatggcgccg acagcggcag cgccggacac
     1741 cctcacctat ttgcgttacg aggatttggt aaagaagtgc gcgacacccc agtggagaat
     1801 tgacggacgt ttgagcgtgt tcgaaggaaa cgacgacacc cagctcggag cggagcgatt
     1861 ccgcacgctg cggtcgcggc tgtatcagat tgcaggaacc cggccgctgc gtcgcgtaat
     1921 ggtgacgagc agcgtgccgg cggaaggaaa aacattcgta gcggcgaacc tggcacaatc
     1981 gatcgtgcgg cagccggatc gcaaagtgtt gctgatcgac gcagacttgc gcgcttcgcg
     2041 cttgcatcag gtgctgggag cgccgcggtc acccggactc tccgactatc tgatcggcga
     2101 ggcggacgag ttccgggtcg tacagaaggg ctcggacgcg aacctgtgct ttatcccggg
     2161 cgggcgcgag gttccgaatc ccagcgaatt gctgatgaac gaccgcttga agaagctgct
     2221 ggagctcatg actccgctgt ttgactggat cattctggat acgccaccgg tactaccggt
     2281 tcacgatgcc agtatgatgg cggatgtttg cgacggagta ttgtttgtag tgcgcgctgg
     2341 aatgactgac cacgaaatgg cggccaaagc gacggcagag tttcaggacc ggaatttgct
     2401 gggcgtggtg ctaaaccgcg tggaagatgg agcgggttac ggcgggtatt attacgggta
     2461 cccagcgggc ggtgagaagg attaagcagt tccggcaggt actgcagggg gtttacgttc
     2521 gccgcgttcg agccaccaca gcaacggggc caaaatagcc aggccgataa gaaagaaaac
     2581 cacgccgcct tcacggtgca ggtttccata caaaaatccg ggatcgacat aactggccag
     2641 gagcgtcagc gtgacgatgc ggatcgcatt cttcaggatc atcacaaaaa ggccagccaa
     2701 gaccagcacc acttgccgcc aaaatgtgcg caggtagaag tgcgccacca ggatggccag
     2761 gatgatcagc gccaggctgg aacgaatgcc actgcattcg cgggcaactt caatgttgac
     2821 gcgcgcgagg tggaagacaa agccatcgcg aaggacggga acaccgctcc aa
//
LOCUS       DASTPM010000495         4125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_104097, whole genome shotgun sequence.
ACCESSION   DASTPM010000495 DASTPM010000000
VERSION     DASTPM010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104097"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VFR42_08805"
     CDS             complement(<1..1227)
                     /locus_tag="VFR42_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEU0369295.1"
                     /translation="MATAQDNPAVSTSTFSAEEQHDRENAKRLAQRYRVPFVDLREQR
                     IDPELFRTIAADLMFRYNFVPLEQRDNLLVIAAADPSLVLLSDELSLLLGKKLAPRVA
                     TAKQIGDLLKRTEQSQRVLEQATEAFTLQVTKTEEDTEETISGDRLTRDTTGSPVVKL
                     VETVIFTALERRASDIHIEARDAEVAVKYRIDGVLQHAMQPISKEWHSAVLSRIKVLS
                     ELDIAERRVPQDGRFRVKYKGRFIDLRVSIMPASHGEDAVLRVLDKETLSEKFQSLSL
                     DVVGFSQEEMQRFRRYIREPYGMVLVTGPTGSGKTTTLYAAINEIKTDEDKIITIEDP
                     VEYQLRGITQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDNETAQIAIQSALTGH
                     LVFTTVHANNVTDVLGR"
     gene            complement(1234..2451)
                     /locus_tag="VFR42_08810"
     CDS             complement(1234..2451)
                     /locus_tag="VFR42_08810"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0369296.1"
                     /translation="MGEFVCRVADANGRVYSHVEAAGTQAEVRQKLADRGLYVYSVEN
                     RSSLLSGILQPQSSRRIGGSDFLILNQQFNTLIKAGLPILRALDLLAERAASPRLRPI
                     VAQIRDRVREGKSLSEAVNEAGVFSKVFSTAILAGEKSGNLPGVLDYYIAYQKVSTGV
                     RKKILATLVYPALLVTVAILVVTYLVTVVVPKFAALYRDMNVELPAATRLLIALTVDY
                     RGTILIGIGGLIALLLGVFFWSRSDEGGEAFDKLKFRLPVVGDTIMKFQVSQFCRTLA
                     TLLTGGTPLVAGLQTAGDAIDSPLLRSSVTRATQMVREGDSLNAALASTRIMPELALD
                     MIEVGESSGALAPMLTSVAEFYEEEVNIRLSALVALIEPAILIFMAILVAFILISLYL
                     PIFSFSISGAAGR"
     gene            complement(2540..3151)
                     /locus_tag="VFR42_08815"
     CDS             complement(2540..3151)
                     /locus_tag="VFR42_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEU0369297.1"
                     /translation="MRAAFLERYFETGREVRSEAGIAYFGRPGKMRWEYRSPEPNLYV
                     IDGKWSWFYVPTDHTVTRIRAKQSSDWRTPLALLAGEMKVSRICARVAVDTSTRAVIA
                     SNVVLRCELRGSAPARRDKDGSPVSETDSDRVLFEVNPVNGELARILVTDPGGVQVEF
                     QFTNWQFDPQLDSKLFRFEPPKGVAIVDGDLAASGSSLVPSAP"
     gene            complement(3310..3789)
                     /locus_tag="VFR42_08820"
     CDS             complement(3310..3789)
                     /locus_tag="VFR42_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0369298.1"
                     /translation="MKRPQKGFSLIELLIVVAIILIIAAIAIPNLLRARMQANEASAA
                     SSIHALNTAEISYSTTFSGIGYSVALADLGDGGTSPCPGTATASCYLDTSLASGTKSG
                     YTFTYTQDSTSTPSLHYPVLVDPVSRGTTGQRSFYSSDSNLTRYNTGITASSTDSPL"
BASE COUNT          932 a         1194 c         1142 g          857 t
ORIGIN      
        1 acgcccgagc acgtcggtga cgttattggc gtgaacggtg gtgaacacca agtggccggt
       61 gagcgcggat tgaatcgcga tttgcgcggt ttcattgtcg cggatttcgc cgaccatgat
      121 tttgtccggg tcgtggcgca aaatcgaccg taacccacga gcaaacgtca gccccttctt
      181 ctcgttcacc ggaatttggg tgatgccgcg caactggtat tcgaccgggt cttcgatggt
      241 gatgattttg tcttcgtcgg tcttgatttc gttaatcgcc gcgtacagcg tggtggtctt
      301 tccggaacct gtcggtcctg tgaccagcac catgccatag ggttcgcgaa tgtagcgccg
      361 aaagcgctgc atctcttcct gcgaaaagcc aacgacgtcc aggctaaggc tctggaattt
      421 ttccgaaaga gtctctttgt ccagtacacg aagaaccgcg tcttcgccgt ggctggccgg
      481 catgatcgaa acgcgcaaat caatgaagcg gcccttgtac ttcacgcgga agcggccgtc
      541 ctgtggaacg cggcgttccg cgatgtccag ctcactcagc accttgatac gcgagaggac
      601 ggccgaatgc cattctttgg aaataggctg catggcatgc tgcaagacgc cgtcgatgcg
      661 gtacttcacc gccacttcag cgtcgcgcgc ttcaatgtga atgtcgctgg cgcgccgctc
      721 caaggctgtg aagatgactg tttccaccag cttgaccacc gggctgcccg tggtgtcgcg
      781 cgtaaggcgg tcgcccgaaa tcgtctcttc tgtatcttct tcggtcttgg tgacctggag
      841 agtaaaagcc tcagtcgcct gctccagcac ccgctgcgac tgttccgtgc gtttcaacaa
      901 gtcgccgatt tgcttggccg tggcgactcg cggcgccaac ttcttgccca gcaacagcga
      961 aagctcatcg cttagcaata ccaaactggg atcggcggcg gcgataacca gcaggttgtc
     1021 tcgctgttcc agcggcacga agttgtaacg aaacatcaag tcggcggcaa tggtccggaa
     1081 aagctctgga tcgatgcgct gttcgcgcaa atccacgaac ggcacgcgat agcgctgcgc
     1141 caggcgcttc gcattctcgc gatcgtgctg ttcttcggcc gagaaggtgc tcgtcgaaac
     1201 cgccggattg tcctgcgcag tcgccatata tacctaacgc cccgctgccc ccgaaatgga
     1261 aaaggagaag ataggcaagt aaagtgaaat caagatgaac gcaaccaaaa tagccataaa
     1321 aatcaaaatg gccggctcaa tcaatgccac caaagccgaa agccggatgt ttacttcctc
     1381 ctcgtaaaat tccgccacgc tggttagcat cggagccagc gcgccgctcg actcgcccac
     1441 ctcaatcatg tccaaagcca gttcgggcat gatgcgggtc gaagccagcg ccgcattcaa
     1501 cgagtcgccc tcgcgcacca tttgcgtggc cctggtgacc gaggagcgca gcaatggact
     1561 gtcaatcgca tccccggcag tttgcaggcc cgccaccaac ggtgtgccgc cggttaaaag
     1621 tgtcgccagc gtgcggcaga actgcgatac ctgaaacttc atgatggtgt caccgaccac
     1681 aggtaagcgg aatttcagct tgtcaaaggc ctcgccgcct tcgtcggagc gcgaccagaa
     1741 aaatactccc aagagaaggg cgatcaaccc accgatgccg atcaggattg tgccccgata
     1801 atccacagta agcgcgatca gcagccgagt ggctgccggc aactccacat tcatgtcacg
     1861 atagagagcg gcaaactttg gcaccaccac agtaaccagg taggtcacca cgagaatggc
     1921 caccgtcacc agcagtgcag ggtataccaa cgtcgccaga atcttcttgc gcacgccagt
     1981 gctgactttc tggtaggcaa tgtaatagtc cagcacgcct ggcaggttgc cgctcttctc
     2041 tccggccagg atcgccgtag agaacacctt cgagaaaacg ccggcttcat tgaccgcttc
     2101 cgaaagcgat ttaccttctc gaacgcgatc gcgaatttgc gcaacgatcg ggcgcagccg
     2161 gggcgaagcg gcgcgttcgg caagcaaatc cagggcgcga aggataggca aaccagcttt
     2221 aatcagggta ttgaactgct ggttgaggat caagaaatcg gaaccgccaa tcctgcgcga
     2281 actctgcggc tgtaggattc cgctcagcaa actgctgcgg ttctccaccg agtacacgta
     2341 tagcccgcgg tccgcaagtt tctgacgcac ttccgcttgc gtgccggcag cttctacgtg
     2401 cgaatagacc cggccgttgg cgtccgccac acggcacaca aattccccca taggctctta
     2461 gtttacctca tctgaaagat atgcagggta acgtgtaagg gttggccgtt tccaggaaag
     2521 tacggtcgcg atgccctggc taaggagcgc tgggaaccag cgaggaaccg gacgcggcca
     2581 aatcaccatc cacgatcgca acgccttttg gcggttcgaa gcgaaatagc ttggagtcca
     2641 actgcgggtc aaattgccag tttgtgaact ggaattcgac ctgcacgcct cccggatcag
     2701 ttaccagaat gcgcgcgagt tctccattca ccggattcac ctcaaatagc acccggtcgc
     2761 tgtccgtttc tgagacagga cttccatctt tgtcgcgccg cgcaggagcg cttccccgga
     2821 gttcacaacg cagaaccaca ttcgacgcaa tcacagcccg agtgcttgta tcgaccgcca
     2881 cccgagcgca aatccgggaa actttcattt cgccggcgag cagcgccagg ggcgtacgcc
     2941 aatcagaact ttgctttgcg cggattcgcg tcacggtgtg gtcggtcggc acgtaaaacc
     3001 acgaccactt tccatcgatc acgtacaagt tcggctcagg tgagcggtat tcccaccgca
     3061 ttttgccagg ccgcccgaaa taggcgatac ctgcctccga gcgtacttcc ctgcccgtct
     3121 cgaaataacg ctcgagaaat gctgcgcgca aagttttcgc ctggcgataa gttcgttcaa
     3181 atctctgaat gaggaccgcg gggctaagat tttcaccctg cgagggagtc ggcctcggcg
     3241 ttaaaagcca cccgacggaa aatagggtga gaactagctt caccctttga agtgtaacat
     3301 cggattcggt tagagcggcg aatccgtcga gctggctgtt attcccgtat tgtaacgcgt
     3361 gaggttggag tcactggagt aaaaactgcg ctgtccggta gtcccgcgcg agacgggatc
     3421 gacaaggact gggtagtgga gcgatggcgt cgaggtcgag tcctgcgtgt acgtaaaggt
     3481 gtagcccgat ttcgtaccgc tggccaaact cgtgtccagg taacaagagg ccgtcgcagt
     3541 tcctggacac gggctggttc cgccgtcgcc caggtcagcc agcgccacgg aatatccaat
     3601 accagaaaac gtagtactgt aggaaatctc ggcagtatta agggcgtgga tcgaggacgc
     3661 cgcggaagcc tcgttcgcct gcatccgtgc acgaaggaga ttcgggatcg cgatggcagc
     3721 aataatcagg atgatcgcta ccacgatcaa gagctcgatt aaggaaaaac ctttttgggg
     3781 ccgcttcatg gattttctcc gtccgacgtt gcagcgagcc gaggcccagc agtcagaaac
     3841 gaatctcagg catagtgaat gcaactttct tgccaatttt cacgacttca aaagcctgta
     3901 aactgcttag tttctagtgt ttgcgacgat caaatttcgg aatcgggctc catgacaaat
     3961 cgtgtccatt gggcctcaaa agcgatgccg gatttgggca catcaccaac gcttactcag
     4021 ttaaaaaaaa caaagggcac ggaccaatga tccgtgccct cgaacatccc ttaccgaggt
     4081 tgcttagccg agcggggtgc ttgtcgaggt tgccgctgcg cccgg
//
LOCUS       DASTPM010000496         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_75154, whole genome shotgun sequence.
ACCESSION   DASTPM010000496 DASTPM010000000
VERSION     DASTPM010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75154"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /gene="rpmE"
                     /locus_tag="VFR42_08825"
     CDS             <1..177
                     /gene="rpmE"
                     /locus_tag="VFR42_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU0369299.1"
                     /translation="NNFQTRSTIKGDRLNVEICSNCHPFFTGKQKLIDTAGRVERFTK
                     KYADAAAKTAAAKK"
     gene            267..1859
                     /locus_tag="VFR42_08830"
     CDS             267..1859
                     /locus_tag="VFR42_08830"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU0369300.1"
                     /translation="MATQSRMFHSENPGEGVESAPPRVTLRNAFAHPFDSAMAAARTC
                     YSPRLIGPEEITDKQRLNIGAGTFFSGHHTVYQHAHFEFGLENVSRQFVWSFLHAHPF
                     YNSEQQSQRYVRMDRVQAYVPSVSLFFDAESRLTYERAIVRAWSYYRELSSLLLPDAR
                     EILQDIWHVGPMSHPKRIQKIERSAEKRSIEIARYVLPVAAFTAMVHTVSGIVLHRLW
                     RMCAASDTPSEARAVVGDMVAHVKNIDAQFFDRFGIEPLDELPEWRATAGSDTTNGDA
                     FAKEFDAQLNGRTAKLLDYSANAPAVIASSYRAVLGLSDPECSDAEAIDRLLNPARNL
                     YRLETLNVGVHAPMMRTLQHANYTFAKKLSHTADCQDQRHRMIPGSRPLLTLADTREP
                     DYITPALLKNNPRAKEVYDRAMQDAWHSKNQLLDRGVPLEIALYLLPNAKSIRLVETG
                     SLLHLLHKWTMRTCFNAQEEIYQSSLDEIAQLREVHPQLTKHIGPPCHLRAGVTTPIC
                     TEGSHFCGVKVWLDFPNVTRRI"
     gene            1973..2608
                     /locus_tag="VFR42_08835"
     CDS             1973..2608
                     /locus_tag="VFR42_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369301.1"
                     /translation="MKILLAFLMALQPLFAFSTPLSEQAIREAYFLGQRRDESTAAFL
                     SKYIHELPPPNYGPHILSITTYTPFAQAVLDSSRHTANYSAQQAQLDHRHATEVVQIL
                     VAIRFTDSYGPMLIDSNPPGGRNAQMIPRPSDFWRDFNVAVFSDDKSLEPTSYDGHPD
                     YYCNEGGCYLIGATIQLEFPAESFPSQNITVQVDPPDADGVAADFDLGLLR"
BASE COUNT          582 a         1144 c          624 g          608 t
ORIGIN      
        1 aacaacttcc agacccgctc cacgatcaag ggcgaccggc ttaacgtcga aatctgctct
       61 aactgccacc cgttcttcac cggcaagcag aaactcattg acaccgccgg gcgagtcgag
      121 cgcttcacca agaagtacgc cgatgccgcc gcgaaaaccg ctgccgccaa aaagtaaagt
      181 tcgaacctcg caattcgacc cgaaattcga ttttcgtctt tcgcatttcg tttgcgcgtt
      241 ccacccatgt agaatcactc tccaccgtgg ccactcaatc acgcatgttc cattcggaga
      301 atcccggcga aggcgttgag tcggcccccc cgcgcgtgac cctccgcaac gccttcgcgc
      361 atcccttcga ctccgccatg gccgccgctc gaacctgcta ctcaccgcgt ctcattggcc
      421 cggaagaaat caccgacaag cagcgtctca acatcggcgc tggtactttc ttcagcggac
      481 accacaccgt ctaccagcac gcgcatttcg aattcggcct ggaaaatgtc agccgccagt
      541 ttgtctggtc cttccttcat gcgcatcctt tctacaattc cgagcagcaa tcccagcgct
      601 acgtccgcat ggatcgcgtg caagcctacg tcccgtcagt ttctcttttc ttcgacgccg
      661 aatcccgcct cacctacgaa cgcgccattg ttcgcgcctg gtcgtactat cgcgaactct
      721 cttcgctgct cctgcccgat gcgcgcgaaa ttctccagga catctggcac gtcggcccca
      781 tgtcccatcc caagcgcatt cagaaaatcg agcgctccgc ggaaaaacgc tccatcgaaa
      841 tcgcccgcta cgttctcccc gtcgccgcgt tcacggcgat ggtccacacc gtctcgggca
      901 ttgtcctcca tcgcctctgg cgtatgtgcg ccgcgtccga cacgcccagc gaagctcgcg
      961 ccgtcgtcgg cgacatggtc gctcacgtca aaaacattga cgcgcaattt ttcgatcgtt
     1021 ttgggattga accgctcgat gaacttcccg agtggcgcgc gaccgccggt agcgacacga
     1081 caaacggcga cgccttcgcc aaagaattcg acgcccagct caacggtcgc accgccaagc
     1141 tcctcgacta ttccgccaac gcccccgccg tgatcgcatc atcttatcgc gcggtcctcg
     1201 gcctctccga tcccgaatgt tccgacgctg aagccatcga tcgtcttctc aatcctgcgc
     1261 gcaacctcta tcgcctcgaa actcttaacg tcggcgttca cgcacccatg atgcgcacgc
     1321 tgcagcacgc caattacaca ttcgccaaaa aactcagcca cactgccgat tgtcaagacc
     1381 agcgccatcg tatgatcccc ggctcgcgcc cgctgctcac cctcgccgac acgcgcgagc
     1441 ccgactacat cactcccgct ctactcaaaa acaatccgcg tgcaaaagaa gtctacgacc
     1501 gcgccatgca agacgcctgg cactccaaaa atcagctcct cgaccgcggc gtccctctcg
     1561 aaatagcgct ctatcttctc cccaacgcca aatccattcg cctcgtcgaa accggttcgc
     1621 tcctgcatct tctccacaaa tggaccatgc gcacctgctt caacgcccag gaagaaatct
     1681 accaatcctc gctcgacgaa atcgcccaac tccgcgaggt ccatccccaa ctcaccaagc
     1741 acatcggccc tccctgccac ctccgcgccg gggtcaccac ccccatctgc accgaaggct
     1801 cccacttctg cggcgtcaaa gtctggctgg acttccccaa cgtcacccgc cgcatctgac
     1861 tgtggcggcc gccttcagat ggcctttcct cgaacacgac cacgcacaat tggtctaaac
     1921 tgttgtcatt cgcttcgcgg taaccacccc ctggcagctg cgggagccgc atatgaaaat
     1981 tctcctggcc tttctcatgg cccttcaacc tctcttcgcc ttctccactc ctctctccga
     2041 acaagccatc cgcgaagctt acttcctcgg ccagcgccgt gacgaatcga cggccgcctt
     2101 tctctccaaa tacattcacg aactgccgcc gcccaactac ggcccgcaca tcctctccat
     2161 caccacctac acgcccttcg cgcaagccgt tctcgattcc agccgccaca ccgccaacta
     2221 cagcgcgcag caggcccagc tcgatcaccg ccacgcgacc gaagtcgtcc agatcctcgt
     2281 cgccatccgt ttcacagact cctacggccc catgttgatc gattccaatc cgcccggcgg
     2341 ccggaacgcc cagatgatcc ctcgcccttc cgatttctgg cgcgatttca acgtcgccgt
     2401 tttctccgac gacaaatctc tcgagcccac ttcttacgac ggccatcccg attattactg
     2461 caatgaaggc ggctgctacc tcatcggcgc caccattcag ctcgaattcc ccgccgagtc
     2521 cttcccctcg caaaacatca ccgtccaagt cgacccgccc gacgccgacg gcgttgccgc
     2581 cgacttcgac ctcggcctat tgcgctaaaa gtagtagcgg cttgtccgcc ccgggcgagc
     2641 tttatgccgc cgctcatctg ccttcgccac gacctgtcct tccggccatt cctaacgctg
     2701 tttcctctta ataaatgtcc gtttgcccac cttcactgcc tttctccgcc cgcactcgca
     2761 atttttgctt acactgcacc taacgcctcg catcgttctc ggagcaggac aggtacccac
     2821 gtgcgacttt ttttccgctc gccgctactc ttcgccgcat tccttgcatg cgccgccgcg
     2881 ctgctgtttc ctcatcacct ccaagcccag tcgccgccgg cccccgccac caacgacgat
     2941 cccgatgcct acgacgaa
//
LOCUS       DASTPM010000497         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_75178, whole genome shotgun sequence.
ACCESSION   DASTPM010000497 DASTPM010000000
VERSION     DASTPM010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75178"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /gene="dnaE"
                     /locus_tag="VFR42_08840"
     CDS             <1..1575
                     /gene="dnaE"
                     /locus_tag="VFR42_08840"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU0369302.1"
                     /translation="LTALNALYRPGPIQGGMIDDFIDRKHGRKKIEYELPELKEILEE
                     TLGVIVYQEQVMQIANRLAGYSLGEADLLRRAMGKKQADEMAKQRERFVEGAAQRNFP
                     AKKIEKIFDLMAQFAGYGFNKSHSAAYALLAYQTAYLKTHYPVEFMAALLTSVTGNTD
                     DVVKYINECREMGIAVEPPDINVSDANFTPHGSAIRFGLAAVKNVGHNAIESIVAGRK
                     KLGRYRDIFEFAESVDLRLLNKRVLESLIKSGAMDSLGRRAQLMAVIDPAIERAQKAQ
                     RDAESGQHGLFGVFEQEDTSSPHEQLPNVPDWDEHQRLAAEKEILGFFITGHPLERYR
                     DKLEGLNALGIAEISGMKSSTGKDETITTAGIICNVRVLKSKKGDFYAQTNLEDMSGS
                     IEMIVFPEAYRRLSEKVKLEVPVLVKAGVRVEEGSNPKINASEIIPLEDAKVPLPRSL
                     RIRIPVEGCNESTVDALHLLFNERKGEARVLFDVERAGDFMVVMEAEGYNVQPDRNFI
                     NRVEELCGRGSVRIIN"
     gene            1747..>2645
                     /locus_tag="VFR42_08845"
     CDS             1747..>2645
                     /locus_tag="VFR42_08845"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU0369303.1"
                     /translation="MKAEQELARIEDQIEHLQAIPGQNDETRRQVEQLHQRVEALRHQ
                     IHSSYGAWEKTELARHPQRPYTLDYVERIFTDWSEIHGDRGFADDPAIVAGMARFHGD
                     EVVVIGQQKARDAKQRVYRNFGMPHPEGYRKALRVMHLAEKFERPIFTFVDTDGAHPG
                     LGAEERGQGEAIALNLREMARLKVPVIATVIGVGGSGGALAIAVADRVLMMENAVYSV
                     ISPEGCASILWKTAERASDAAEALGLTAHRLKAMNLIDKIVSEPLGGAHRDPKQMAAM
                     LKRALADTLRQYQGVKTRDLLAAR"
BASE COUNT          600 a          725 c          850 g          470 t
ORIGIN      
        1 ctcaccgctc tgaacgcgct gtaccgtccc gggccgattc agggtggaat gatcgacgac
       61 ttcatcgatc gcaagcatgg ccgcaagaag atcgagtatg agcttcctga attgaaggaa
      121 atcctggaag agacgctggg cgtcatcgtg taccaggaac aagtcatgca gatcgccaac
      181 cggctggcgg gctactccct gggcgaagct gacctgctgc ggcgggcgat gggcaagaag
      241 caggccgacg agatggcgaa acagcgcgaa cgtttcgtgg aaggtgccgc acaacgcaac
      301 ttcccggcga agaagatcga gaagatcttc gacctgatgg cgcagtttgc cgggtacggc
      361 ttcaacaagt cgcactccgc ggcctacgca ttgctcgcat accagacggc ttatttgaag
      421 acgcactacc ccgtggagtt catggcagcg ctgctgactt cggtgaccgg caacacggat
      481 gacgtggtca agtacatcaa cgaatgccgc gagatgggaa ttgcggtcga gccgccggat
      541 atcaacgtgt cggatgcgaa ctttacgccg catggaagcg cgattcgttt cggcctggcc
      601 gccgtgaaga acgttggcca caacgcgatc gagtcgattg ttgccggccg caagaagctg
      661 gggcgctatc gcgacatttt cgagtttgcc gaaagcgtcg acctgcggct gctgaacaag
      721 cgtgtgctcg agtccctgat caagagcgga gcgatggatt cgctgggccg gcgtgctcaa
      781 ctgatggcgg tcatcgatcc cgccattgag cgcgcacaaa aagcacagcg ggacgccgaa
      841 tccgggcagc acgggttgtt cggtgtgttc gagcaggaag acacgtcatc accccatgaa
      901 cagcttccca acgttcctga ctgggacgag catcagcgcc tggctgccga gaaggaaatc
      961 ctcggcttct tcatcaccgg ccacccgctc gaaagatacc gcgacaagct cgaaggattg
     1021 aatgcgctag gcatcgcgga aattagcgga atgaagtcat ccaccggtaa agacgagacg
     1081 atcacgaccg ccggcatcat ctgcaacgtg cgcgtgctga aatcaaagaa aggagatttc
     1141 tacgcgcaga cgaatctgga agacatgtcg ggatccatcg agatgattgt gtttccggaa
     1201 gcgtaccggc gactgtcgga aaaggtgaag ctggaggtac cagtgctggt gaaggccggc
     1261 gtgcgtgtgg aggaaggatc aaatccaaaa attaacgcgt cggagattat tccactggaa
     1321 gacgcgaagg ttccgttgcc ccggtcgctg cgcattcgca tacctgtcga gggctgcaac
     1381 gaatcaaccg tggacgcgct gcacctgttg ttcaacgaac gcaagggcga ggcgcgggtg
     1441 ttattcgacg tggaacgggc gggcgatttc atggtggtca tggaagccga aggctataat
     1501 gtccaaccgg atcgcaattt catcaaccgc gtggaagagc tgtgcggtcg aggcagtgtt
     1561 cgcatcatca attgagatcg gcggcggatg agagttccaa aaatcaggaa atcgagtgcc
     1621 gagaagcatc cgcttcccat gcctgcagac ggggcccagg tggtgacggg ggcagaattc
     1681 ttgctgtcta tggtggagac gcattggaac tccagaaatg gggatgcccg aaaaatggaa
     1741 gttcgattga aagcggagca ggagttggcg cgcatcgagg atcagatcga gcatttgcag
     1801 gcgattcccg gacagaacga cgaaacgcgc cgccaggtgg aacaactgca ccagcgcgtg
     1861 gaagcgctgc gccaccagat tcactccagt tacggggcct gggaaaagac cgagctagcg
     1921 cggcatccgc agcgtcccta tacgctcgac tacgtggagc gcatcttcac cgactggagc
     1981 gagattcacg gggaccgggg ttttgccgat gatccggcga ttgtcgccgg gatggcgcgc
     2041 tttcatggag acgaagtcgt cgtcatcgga cagcaaaagg cgcgcgatgc gaagcagcgc
     2101 gtgtatcgca attttggaat gccacacccg gaaggctatc gcaaggcgct gcgcgtgatg
     2161 catctggcgg aaaaatttga gcggcccatc ttcacgttcg tcgacacgga cggcgcccat
     2221 cccggactgg gggccgagga acgcgggcag ggcgaagcca tcgcgttaaa tctccgtgag
     2281 atggcacggc tcaaagttcc agtcatcgcg accgtgatcg gtgttggagg cagtggcggc
     2341 gcgctcgcca tcgccgtggc ggatagggtt ttgatgatgg agaacgcggt ctattcggtg
     2401 atctccccgg aaggctgcgc ctcgattctc tggaagaccg ccgagcgggc atccgacgcc
     2461 gccgaggcgc tcggcctgac ggcgcatcgc ctgaaagcca tgaacctgat cgacaagatc
     2521 gtctccgagc cgctcggcgg cgcccatcgc gatccgaagc agatggccgc gatgttgaag
     2581 cgcgccctgg ccgacaccct gcgccagtac cagggcgtga aaacccgcga cctgctggcc
     2641 gcacg
//
LOCUS       DASTPM010000498         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_190827, whole genome shotgun sequence.
ACCESSION   DASTPM010000498 DASTPM010000000
VERSION     DASTPM010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190827"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1992)
                     /gene="uvrC"
                     /locus_tag="VFR42_08850"
     CDS             complement(70..1992)
                     /gene="uvrC"
                     /locus_tag="VFR42_08850"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU0369304.1"
                     /translation="MEPKEKAGLLPEKAGVYLFRDAAEHVIYVGKARLLRNRVRSYFL
                     ESRWMDAKTGSLAREIADLETIVVDNEREALALENQLIKRYKPKFNVMLRDDKTYPYI
                     KYTAAEKYPRVYFTRRIKKDGSLYFGPYFPASLARRILHFVHKRFLVPSCTVDLTRTH
                     PRPCLQYYIHRCLGPCVAGLTTDERYTEAARDVRMLLEGRRHDLIKSLEERMTVAAEK
                     ELYEQAASYRDLLRTLEDIEERQRIAAAQGDDTDVLAYHAEPPLVAANLFHLRAGRVV
                     DRREFYWEDLEEFEASEFVVSLLKQLYLDAEYLPKAIHVPEEFEDRELLEEMLSERAG
                     HRVEILTPQRGAKRAVMDLVKSNAAHSFEQRFRVLKPTSRAIGEALQNALNLAEEPER
                     IECFDISHIQGSDTVASMVVWEKGRMKKSDYRKFIIRGMEAGEFVGAEKDEEKSRQSG
                     ESSSVGDDGVRLLQVYRQNDDFASMREVVSRRYRRVQEEKKTMPSLVLIDGGIGQLHA
                     AADALEGLGIINQPLASIAKKEEVLYVYGQEDEPVVLDRHSPALHLIQQIRDETHRFA
                     VTFHRLRRGKRQTRSALVEIPGVGAKTAQKLLKEFGSVANVRRAGVERLSMVIPRKSA
                     ERMLAKLEEQEVEANQ"
     gene            2106..2630
                     /locus_tag="VFR42_08855"
     CDS             2106..2630
                     /locus_tag="VFR42_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369305.1"
                     /translation="MLRDQDHPQSSARGAPEVSPARQRRLLSARRSLLAPFLLTALSA
                     SCLSAQSAPQDPPKPKPKPNSTTDNATQNAPDQPTWDPLRAEKDIEVGQYYMRKGDVD
                     AAIDRFQDATLAKPGYAIPFRYLGEAQEKKGLKRDAIKSYTRYLDLFPHAEDKSKIEK
                     KIEKLRSEVDKSKK"
     gene            complement(2814..>3224)
                     /locus_tag="VFR42_08860"
     CDS             complement(2814..>3224)
                     /locus_tag="VFR42_08860"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEU0369306.1"
                     /translation="TRQRIQNMRSGRVRRAALDATGRHFDMNAMFDDLNARFFASELR
                     RPHLGWSTRGWRRQFGSYDPGPDQILLNKRMDRPEVPRYVVEYVLYHEMLHVKHPTRK
                     SGCTLLSHSPEFRAEEKLFPEFHRARKALDRLAR"
BASE COUNT          664 a         1127 c          728 g          705 t
ORIGIN      
        1 tctcctccaa atcgcaccgt aacacactga tttgtcagcc acaatacgca ctaaccagtc
       61 tccacctctt cattggttag cttcgacttc ctgctcttcc agcttggcca acatcctttc
      121 cgcgctcttt cgcggaatta ccatgctcaa tcgttccact cccgcccgcc ggacgttcgc
      181 tacgctcccg aactccttca ataacttctg cgcggtcttc gcaccgactc ccggaatctc
      241 taccagtgcg ctccgggtct gccgctttcc ccttcgcagg cggtggaacg tcactgcgaa
      301 tcggtgtgtc tcgtcacgga tctgctggat tagatgcaac gccggcgaat gccggtccag
      361 caccaccggc tcatcctcct gcccgtatac gtacaagacc tcttccttct tcgcgatgct
      421 agccagcggc tggttgatga ttcccagccc ttccagtgcg tctgcggcag cgtgcaactg
      481 tccgatgccg ccgtcaatca ggaccaaact gggcatcgtc tttttttctt cctgcactcg
      541 ccggtaccgc ctcgacacca cttcccgcat gctggcaaaa tcatcgtttt gccgatacac
      601 ctgcaatagt cgaactccgt cgtctccgac ggaactcgat tctcccgact ggcgagattt
      661 ctcttcatcc ttctccgcac cgacaaactc ccctgcttcc attcctcgaa taataaattt
      721 ccggtaatcc gactttttca tccgcccttt ttcccacacc accatcgaag ccacggtatc
      781 gcttccctga atgtgcgaaa tgtcgaaaca ttcgatccgc tccggctctt ccgccaggtt
      841 cagcgcattc tgcagcgcct ctccaatcgc ccgcgaagtt ggcttcagca cacggaaccg
      901 ttgctcaaac gaatgcgccg cattgctttt caccaggtcc atcaccgcgc gtttggctcc
      961 ccgctgcggc gtcagaatct ccacgcgatg cccggcacgc tcgctcagca tctcttccag
     1021 cagctcgcga tcctcgaact cttccggcac gtgaatcgct ttcggtaaat actccgcatc
     1081 cagatatagc tgcttcagca gcgacaccac aaactcactc gcctcaaact cttccaggtc
     1141 ttcccaatag aattcccgcc ggtccaccac tcgcccagcg cgcaagtgaa acaaattcgc
     1201 cgccaccagc ggcggctccg cgtggtacgc cagcacgtcc gtatcatcgc cctgcgctgc
     1261 tgcaattcgc tgcctctcct cgatgtcttc cagcgtgcgc aatagatcgc gatacgatgc
     1321 tgcctgttcg tacaactcct tttccgctgc gaccgtcatc cgctcttcca aactcttgat
     1381 caggtcgtgc cgccgtcctt ccagcagcat ccgcacatcc cgcgccgctt cggtatatcg
     1441 ctcatccgtg gtcagtccgg ccacgcacgg ccccagacac cggtgaatgt agtactgcag
     1501 acaaggccgc gggtgcgttc gcgtcaaatc caccgtgcac gaaggcacca gaaaacgttt
     1561 gtgtacaaaa tgtagaatcc gccgcgccag gcttgccgga aagtacggcc cgaaatacag
     1621 agagccgtct ttcttgatgc gccgcgtgaa atatactcgc ggatacttct ccgccgcggt
     1681 gtacttgatg tacgggtagg tcttgtcgtc ccgcagcatc acgttgaatt tcggcttgta
     1741 ccgcttgatc agctgatttt cgagcgccag tgcctcgcgc tcgttgtcca ccacaatcgt
     1801 ttccagatcc gcaatttccc gcgccagcga acccgtcttc gcgtccatcc atcgcgattc
     1861 cagaaaatac gaacgcaccc ggttccgcag cagccgcgct ttgcccacat aaatcacatg
     1921 ctccgccgcg tcgcggaaca ggtacacccc agccttctcc ggtagcagcc ccgctttttc
     1981 tttgggttcc atcccgatag ccgtagtagc ggtcgccttc tgaggcgggc gcttttcgag
     2041 cgccagatag ggcgcctcta ctcccgcgag ccaacttcca tcatactaga cggaatcaaa
     2101 ctgccatgct tcgtgaccaa gaccatccgc aaagcagcgc ccgaggcgcg ccagaagtta
     2161 gcccagcgcg acagcggcgc ctgctatctg ctcgccgttc gttgctggcc ccctttcttt
     2221 tgactgcgct ctccgcttca tgcctctccg cgcaatctgc gcctcaagat ccccccaaac
     2281 cgaaaccaaa acccaattcc accaccgaca acgccacgca aaatgccccc gaccaaccca
     2341 cctgggaccc gctgcgcgca gaaaaagaca tcgaagtcgg ccaatactac atgcgcaaag
     2401 gcgacgtcga cgccgccatc gatcgtttcc aggacgccac actcgccaaa cccggctacg
     2461 ccatcccatt ccgttattta ggggaagccc aggagaaaaa agggctgaag cgcgacgcca
     2521 tcaaatccta cactcgctat ctcgatcttt ttccccacgc cgaagacaaa tccaaaatcg
     2581 agaaaaaaat cgaaaagctc cgctccgaag tcgacaaatc taaaaaatag gtttcgggct
     2641 gtctctaggg tccgtttatg tttcaattcg ctaaagggat ggtcattccc aagcggagtc
     2701 tgcggagcga ggaatctccc ttcgatgctc agcttgagaa cgcaacctga agcggcggcc
     2761 gcctattttg cccttctcct aggggacaac tgaaaactga aggctggtaa cctctatcgc
     2821 gctagccgat ccagcgcctt tcgagcgcga tggaattctg ggaatagctt ctcctccgct
     2881 cgaaattccg gcgaatgcga cagcagcgtg catccggact tccgcgtcgg atgcttcacg
     2941 tgcagcatct cgtgatacag cacgtactcc accacatagc gcggcacttc cggccgatcc
     3001 atccgcttgt tcagcaaaat ctgatccggc cccggatcat acgagccgaa ctgtcgccgc
     3061 cacccgcgcg tgctccatcc gagatgcggc ctccgcaatt cgctcgcaaa aaaacgcgcg
     3121 ttcagatcat caaacatcgc attcatatcg aaatggcgtc ccgtcgcgtc cagagccgca
     3181 cgccgcacgc gtccgctgcg catgttttga atccgctgcc gcgt
//
LOCUS       DASTPM010000499         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_796453, whole genome shotgun sequence.
ACCESSION   DASTPM010000499 DASTPM010000000
VERSION     DASTPM010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796453"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VFR42_08865"
     CDS             complement(<1..1083)
                     /locus_tag="VFR42_08865"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEU0369307.1"
                     /translation="MTTFFRKLPFGCALTLVCLIFVGQVSGQASGQSSPPVANRITQV
                     PDNSTRITLKGGVHPLARAEFSRGAVADSLAIDRILLLLKRSDQQEAALESLLESQQD
                     KFSPNYHRWLTPEAFGQQFGPSDADMQSVTDWLTSQGFHDVRVGPGRSVIEFSGNAAQ
                     VRQAFLTQIQSYAIKDAAYVANSSDPQIPAALAPVISGIVSLHNFPKKSHSRFLGDVR
                     RDPNSRKLEPLFTFPNPNNGKNFYGVGPGDFANIYNSGTLVNSGNDGTGQTIAIVGET
                     QIDPTDVSDFRAMFGLSNNFASSNIVLNGMDPGITSRDEESESDLDVQWSGAVAPGAT
                     VKFVVSASTPASAGIDLSAFYIIEHNL"
     gene            complement(1310..2296)
                     /locus_tag="VFR42_08870"
     CDS             complement(1310..2296)
                     /locus_tag="VFR42_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0369308.1"
                     /translation="MSSKTIAPATVPTWEEIQRAHQRIAPKTHRTPVLRSATLDALCG
                     ADLFFKCENLQKTGSFKIRGATNAIFSLSDEEAARGIVTHSSGNHAAAVSCAAAWRGV
                     PAWIVMPKNAPVVKRKAVEAYGGKITFCEPIVSARSETAARIQSETGAVMIHPYDDDR
                     VIAGQATAAKELLEDVGELDAILSPVSGGGLLSGTSLSAKHLRPSIKIYGCEPAKADD
                     AYRSLQTGVLQAMESSDTIADGLRASLAPRTFAILRELLSGILLVSEEEIIGAMELIW
                     ERLKVITEPSSAIALAPLLKPGAAASLFPETARPKIGLLLSGGNVDFRSLPF"
     gene            2565..2912
                     /locus_tag="VFR42_08875"
     CDS             2565..2912
                     /locus_tag="VFR42_08875"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0369309.1"
                     /translation="MESSERRQLIRYAMRTPIRFKAMGLAADKSEHFTEALNVSRGGF
                     YFATSAPLQIGMPIEATLRMPEEVTGEKTKETHCRARVVHVRNEPYSDGRIGFGAEIE
                     SFLSMPRPDRWAG"
     gene            complement(2987..>4778)
                     /locus_tag="VFR42_08880"
     CDS             complement(2987..>4778)
                     /locus_tag="VFR42_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369310.1"
                     /translation="VTPDGKSELFYASDEAHIRVLAFDGKANLIAGTEPSGRVLRLTR
                     PASSGRKNEKDPAAEGFVLYETPKREVTSLAVGSDGSIYVAAIGERQHNAAVISTVPG
                     QTTTSTTTVTTSGNVTVIAPIQSTGTGQPQAPYIPFPSAISSGVYKIAPDGAPEELWT
                     SRDDVVYSLGIGNDGRLVAGLGNSGALLAIDGHGVFAQLAKAGSAQITGITRNSVGKV
                     ILCTANPGKLISLGPEYEPEGTYESRSFDAQLFSQWGRIEWWSPPAMTTASAKNSGAN
                     SREPRLEFYVRTGNTEDPGKEWSKWYGPFSSSGAETGAPSARFAQWKAVIHDGRPGDG
                     IEWVTLAYQPKNVSPVIDGILLQDPGVRAQGPPALATGAAPAATLKFPPAPSPSSGVI
                     LSQSQNSSLSKFEVPPQGFVQKGYQSVLWTAHDDNDDDLKYSVYFRGEAEKDWKLLKD
                     NLEQKFYSFDTTTLADGAYYLKIVATDAASNPPDKAKKTERESERFEVDNTPPAISKV
                     DAVAANATAQVQFSAADATSSIDRAQYSLDGGEWTLVAPKSGISDGKQQSYEFALRDL
                     KPGEHTIALRAYDRFENVGTGKAVVSIPAK"
BASE COUNT          938 a         1283 c         1533 g         1024 t
ORIGIN      
        1 caggttgtgc tcaatgatgt agaaagcgga aaggtcaatg ccggcgctgg ccggcgtgga
       61 agcggaaacc acaaatttta cggtagcgcc gggcgctacg gcacctgacc attgcacgtc
      121 aagatcggac tcggattctt catcccgcga ggtgatgccg gggtccatgc cattaagaac
      181 gatgttcgag gacgcaaaat tgttggaaag accgaacatg gcgcggaagt cgctgacatc
      241 ggtgggatct atttgcgttt cgccgacgat ggcgatggtt tgtccggtgc cgtcattgcc
      301 ggaatttacc aaggtcccgg agttgtaaat gtttgcgaag tcgcccggcc caacgccgta
      361 gaagtttttt ccgttgttcg gattgggaaa cgtgaaaaga ggttcgagct tgcggctgtt
      421 ggggtcccgg cgaacgtcgc ccaggaagcg gctatgcgat ttcttgggaa aattgtgcag
      481 cgaaacgatt ccggaaatta ccggagcgag cgccgccgga atttgcggat cagatgaatt
      541 ggcgacgtaa gccgcgtcct tgatcgcata gctctggatt tgcgtcagaa acgcctggcg
      601 aacttgtgct gcatttccgc tgaattcgat gacgctgcgg ccaggtccta cgcggacgtc
      661 atggaagcct tgcgacgtca gccagtcggt gacggattgc atgtcggcgt cggaagggcc
      721 gaactgttgg ccaaatgctt ccggcgtgag ccaccggtga tagttgggag agaatttgtc
      781 ctgctgagat tccagcagcg attctagcgc cgcttcttgc tggtcacttc gtttcagcag
      841 aagcagaatg cggtctatag caagagagtc ggcaacggcg ccgcggctga attcggcacg
      901 ggctaacggg tgtacgccgc ccttgagggt gatgcgcgtg gaattgtccg gaacttgcgt
      961 aatgcgattg gcaactggcg gggagctttg gccgctcgcc tggccgctca cttggccaac
     1021 aaaaatcaaa cacacaagcg tcaacgcaca cccgaacggg agcttgcgaa aaaatgtggt
     1081 catttatggg tacccgaacg tcctatttgc acacaaggtt cgaccgtgaa accttgcgga
     1141 atgctgcact cacggcttcg ggcgagccgc ggaacccagc ctctttacgc cttcctgaga
     1201 tccctcaggt aaagaggctc cgcggagtct gccagtgtgc cggaaggcgg catcgactcc
     1261 gaacgggcga agagtaccac aggaaatcga taaaatcatt agaacgtgac tagaaaggca
     1321 gcgaacggaa gtccacgtta ccgccggaga gtaggaggcc gattttgggc cttgctgttt
     1381 cggggaaaag ggaagccgcc gcgccgggct tcaagagagg agccagagca atcgcggaag
     1441 agggctcggt gatgacctta agccgctccc agatcagctc catggcgcca atgatttctt
     1501 cctcgctgac cagcagaatg ccggaaagca gttcgcgcaa aatggcgaaa gtacgcggcg
     1561 ccagggaagc gcgcaggccg tcggcgatgg tgtcgctcga ttccattgct tgtagaacgc
     1621 cggtttgcag cgagcggtag gcgtcgtcgg ccttggcggg ctcgcaaccg taaatcttaa
     1681 tggatggtcg caggtgcttt gcgcttagcg aagttccgct gagcaggccg ccgccgctga
     1741 ccggcgacaa gatggcatcg agttcgccaa catcttcgag gagttctttt gcggccgtgg
     1801 cctgaccggc aatgacgcga tcgtcgtcgt agggatgaat catgacggcg ccggtttcgg
     1861 attgaatgcg cgcggcggtt tctgaacgcg cggagactat tggctcgcag aaagtgattt
     1921 tgccgccgta ggcctcgacg gctttgcgct taaccaccgg agcgtttttg ggcatgacaa
     1981 tccatgcggg aacgccgcgc catgcagctg cacaggaaac cgccgcggcg tggttgccgc
     2041 tggagtgcgt gacgatgccg cgggccgctt cttcgtcgct tagcgagaag atggcgttcg
     2101 ttgcgccacg aattttgaag gagccggtct tttgcaggtt ttcacatttg aagaaaagat
     2161 cggctccgca cagcgcgtcg agcgtggcgg agcgcaacac cggcgtgcga tgagttttcg
     2221 gcgcgattcg ctggtgcgcg cgctgaattt cttcccaggt tggcactgtg gcaggagcga
     2281 tcgttttgga ggacatttac ctgttctggt tcctgaaacg gaaaccacag gttagcgcac
     2341 tatcgtcgga acggcgagtg gcagtggaac gtggcggcgt ggcactgttg gaaaattggc
     2401 cggcgaaatt caggcgaaac caaatcgcgt tctattgccg cagcgagggc gcacttgaag
     2461 ccggatttta acttgaccat gcctcatgct ttgctaatct tgcccatacc cttccgcaga
     2521 tttcggacgc agtcccccct ttcgctgcgt gaaggagcaa cgaaatggaa agctcggaac
     2581 gccgtcagtt aattcgctac gctatgcgga cacccatccg cttcaaggcc atgggcttgg
     2641 ccgcggacaa aagcgaacat ttcaccgaag cattgaacgt ttcgcgtggg ggattctatt
     2701 tcgccacctc agcgccactg caaatcggaa tgccgattga agcgacgctg cggatgccgg
     2761 aagaagtgac cggcgaaaaa acgaaggaaa cgcattgccg cgcgcgcgtg gtgcacgtcc
     2821 gcaacgagcc gtacagcgac gggcgcatcg gcttcggcgc ggagattgag tcgtttctct
     2881 ccatgccgcg gccggatcgc tgggcgggct agttggccat tcacaccgat cgattcatgc
     2941 gataaggcac cgtgtattac gggtcggtcc ggatttttct gttcctctat ttcgcgggaa
     3001 tcgagaccac ggcttttccc gtgcccacgt tttcaaagcg gtcgtaggcg cgaagcgcaa
     3061 tggtgtgttc gccgggtttc agatcgcgca aggcgaattc ataggactgc tgtttgccgt
     3121 cgctgatgcc ggatttcggc gcgaccagtg tccattcgcc gccatcgagg ctgtactgcg
     3181 cgcgatcgat gctggaagtc gcgtcggcgg cgctgaactg gacttgcgct gtggcgttgg
     3241 cggcgaccgc gtccactttg ctgatcgccg gcggcgtgtt gtccacttca aagcgctcgc
     3301 tttcgcgctc ggtcttcttt gctttgtcgg gcgggttgga cgcagcgtcg gtggccacga
     3361 ttttcagata ataggcgccg tcagcgagcg tcgtggtatc aaaagaatag aacttctgct
     3421 ccaggttgtc cttcagcagc ttccaatcct tttcggcttc gccgcgaaaa tagacgctgt
     3481 atttgaggtc atcgtcgttg tcgtcgtgcg cggtccacag gacagactga tagccttttt
     3541 gcacgaagcc ttgcggcggg acttcaaatt tgctgaggct gctgttttgc gactggctga
     3601 ggatgacgcc actcgagggg gacggggccg gcgggaattt cagggttgcc gcgggggctg
     3661 cgccagtcgc cagcgcaggc ggaccttgcg cgcggacgcc cgggtcctgc aacaaaattc
     3721 catcgatcac cggtgagaca tttttcggct gataggccag cgtgacccat tcgatgccgt
     3781 cgccggggcg gccgtcgtgg atgaccgctt tccactgggc gaagcgcgcc gagggcgccc
     3841 cggtttctgc gccgctggag gaaaaaggtc cgtaccactt ggaccattct ttcccggggt
     3901 cttcggtgtt tccggtgcgg acgtaaaact ccaggcgcgg ttccctactg ttggcgccgc
     3961 tgttctttgc ggaggctgtc gtcatggccg gaggactcca ccactcgatg cgtccccatt
     4021 gcgaaaaaag ttgtgcatcg aaagagcgcg actcgtaggt gccttccggt tcgtattcgg
     4081 ggccaagtga aatcagtttg ccgggattgg ccgtgcaaag gatcactttg ccgacggagt
     4141 tgcgggtgat tccggtaatt tgcgcggatc ctgctttagc gagttgcgcg aaaacgccgt
     4201 ggccatcgat ggctagcagg gcgccggagt tgccaaggcc ggccacgagc cggccgtcat
     4261 tgccaatgcc cagcgaatag acaacgtcgt cgcgcgaagt ccaaagttct tccggcgcgc
     4321 catcgggcgc aatcttgtaa acgccactgg aaattgcgga aggaaacgga atgtagggag
     4381 cttgcggctg gccggtgcca gtgctttgta tgggggcaat cacagtgaca ttgccgctgg
     4441 tggtgaccgt ggtagtgctg gtggtggttt ggccgggcac ggtcgagatg acggcggcat
     4501 tgtgctggcg ctcgccgatg gccgccacat agatagagcc atccgagccg acagccagcg
     4561 aagtgacttc gcgtttgggc gtttcgtaga gaacgaagcc ctcagccgcg ggatcctttt
     4621 cattcttgcg accggaactt gctggacggg taagccggag cacgcggccg ctgggttcgg
     4681 tgccggcgat cagatttgcc ttgccgtcaa aagccagcac gcgaatgtgg gcttcatcgc
     4741 tggcatagaa aagttcgctc ttgccgtcgg gcgttacc
//
LOCUS       DASTPM010000500         3467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_421619, whole genome shotgun sequence.
ACCESSION   DASTPM010000500 DASTPM010000000
VERSION     DASTPM010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421619"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFR42_08885"
     CDS             complement(<1..838)
                     /locus_tag="VFR42_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEU0369311.1"
                     /translation="MESAAAPSRIQSVDALRGAIIILMALDHVRDFTSAAAMAFSPTD
                     LSRTSAALFFTRWITHFCAPVFAFTAGLGAFFWYQHGRTLVLLSRFLLTRGLWLMFLE
                     LTLLRFIFFFQWRWSGALFILTVFWMLGLCMVVLAALAHLPVKLLAPLSLLVIAGHNL
                     LDPITPERFGRFAFLWDILHQQAVFPFREALFLVAYPLVPWVAVMASGFCFGRVLLWD
                     GERRRRFLIRLGSALMIAFVILRAGNHYGDPAKWSHQSTALFTALSFLNTTKYPPSLI
                     FLL"
     gene            962..1768
                     /locus_tag="VFR42_08890"
     CDS             962..1768
                     /locus_tag="VFR42_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0369312.1"
                     /translation="MIDNRSAPPGPVVPVLAYDHIEKAIEWLSGAFGFTERLRIMHEG
                     SIHRVQMAAGQGGVVLVPPRKAEQAGKARVFVRVKDAHAHYARAKQAGAKVNHPPQDY
                     EYGERQYAAEDREGNEWTFSQTIADVDPREWGGTPAQIEPMVARLERPRVCYLEIPAK
                     DVRVSATFYEKLFGWNIRNRESGRPSFDDACGVISGAWVTGREPAREGGLLPYIWVDD
                     LRASVGKIGECGGSAVDAPHPDHPGGTSWIATFRDPAGNLLGLYQENVSE"
     gene            complement(1826..>3467)
                     /locus_tag="VFR42_08895"
     CDS             complement(1826..>3467)
                     /locus_tag="VFR42_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU0369313.1"
                     /translation="VFVATLDGKLIALDKRTGAPVWTVDTVDIAKAYSITGAPRIVGS
                     RVLIGNAGAENGVRGYISAYDAETGKLAWRFYTVPGDPSRGFESKSMEDAAKTWRGEW
                     WKVGGGGTVWDSIAYDPALDLVYFGTGNATAWYRALRGGGDNLYTASIVALHAGTGEL
                     AWHFQTSPGDSWDYDATQPLLQAELNIGGRSRKVIMQPNKNGFFYVLDRATGEFLSGT
                     PYVSGITWATGLDPKTGRPIEVAGSAGAGPVMVSPAPGGAHNWNPIAFSPTTGLVYLA
                     AKIGTQMVHVPDPKWKYDPNTQNIGLDQHEGPLHAKLAHMVPATGELLAWDPIARKAA
                     WRAAIPVLEGGGTLATAGNLVFQGRSDGIIAAYRATDGKQLWSFDAGTGIMAPPVTYT
                     VDGVQYISVMVGMGGSPGLYHFPGGGPEKFGYGRIVTFALGGTARLKLRAFGHQDPPV
                     PALAFKATPQQLQAGSLLFSAHCMPCHGFQAIAGSLPDLRYSNKETLLDMENIVLRGT
                     RSAVGMPSFRKELNAKQVANLRAYILSRAAESAKAAAK"
BASE COUNT          776 a          979 c         1084 g          628 t
ORIGIN      
        1 tcaaaagaaa aatcagcgac ggcggatatt ttgtcgtgtt gaggaaagac agcgcggtga
       61 aaagtgccgt cgactgatgc gaccactttg ccgggtcgcc gtagtgattc cccgcccgca
      121 gaatcacgaa cgcgatcatg agtgctgaac cgagacgaat cagaaaacga cggcggcgct
      181 cgccgtccca cagcagcaca cgtccaaagc aaaaacccga ggccatcacg gcgacccacg
      241 gcaccagagg atacgccacc aagaacagcg cttcgcgaaa tggaaaaacc gcctgctggt
      301 gcagaatgtc ccacagaaaa gcaaaacggc cgaaacgttc cggagtgatt ggatcgagca
      361 gattgtgccc ggcgatcacc agcagactga gcggcgcaag gagtttcacc ggcagatgag
      421 ccagcgcggc cagcaccacc atgcaaagcc ccagcatcca gaacaccgtc aggatgaaca
      481 gcgcgccgct ccaacgccat tgaaagaaaa atataaagcg gagcagcgtg agttccagaa
      541 acatcagcca gaggccgcgc gtaagaagga aacgcgacag tagcacgagc gtgcggccat
      601 gctggtacca gaaaaatgcg ccgaggcccg cggtaaacgc aaaaaccggc gcgcagaaat
      661 gcgtgatcca acgcgtaaag aaaagagcag cactggtgcg gctcaagtcg gtcggcgaaa
      721 acgccatcgc cgccgcactg gtgaaatcgc gcacgtggtc cagcgccatc aggatgatga
      781 tggccccgcg cagggcgtcc acagattgaa tacgcgacgg cgcagcggcg gattccatgg
      841 ttgctcgctc acattctatc cactgccact ttcgcgcgag aacacttctt tacagagcag
      901 agctggttcg tgatgatact ggcgtgcaca atgacagttc ggaaaaaaaa gtgaggtgac
      961 catgatcgat aatcgctcgg ctccgcccgg acccgtggtt ccagtgctgg cctatgacca
     1021 tatcgaaaaa gcgatcgagt ggttgagtgg agcgtttgga tttacggagc ggctgcgcat
     1081 catgcatgag ggaagtattc atcgcgtgca gatggccgcg gggcaaggcg gcgtggtgtt
     1141 ggtgccaccg cgcaaagccg agcaagccgg caaggcccgc gtcttcgtgc gcgtgaaaga
     1201 tgcgcacgcg cattacgcgc gggcgaaaca agccggcgca aaagtaaacc atccaccgca
     1261 ggattacgaa tacggcgaac ggcaatacgc ggctgaagac cgcgaaggca acgagtggac
     1321 attttcgcag acaattgcgg acgtagaccc cagggagtgg ggaggaacac ccgcgcagat
     1381 tgaaccgatg gttgcgcggc tggagcggcc gcgcgtttgc tacctggaga ttcctgccaa
     1441 ggacgtcagg gtgtctgcta cgttttacga aaaactgttc ggctggaata tccggaatcg
     1501 cgaaagtgga cgaccgagtt tcgatgacgc ttgcggagtg atcagcggcg cgtgggtcac
     1561 cgggcgcgag ccggcccgcg aaggtggatt gctgccatac atttgggtgg acgatttgcg
     1621 cgctagtgta ggaaagattg gcgaatgcgg cggctcagcg gtggatgcgc cgcatccgga
     1681 tcatccgggc ggaacaagtt ggattgccac ctttcgggat cctgcaggga atttgctcgg
     1741 tctctatcag gagaatgtgt cggagtaatt tccttcgcgg agtcggacca aattctccag
     1801 catggccagg gagctaaagc gtaggttact tggcggctgc tttcgccgac tctgctgcgc
     1861 gcgacagtat gtacgcgcga aggttcgcga cctgtttggc gttgagttcc ttcctgaaag
     1921 aaggcatgcc gaccgccgag cgggttccgc gcaggacgat gttttccata tccaataagg
     1981 tttctttgtt ggagtaacga aggtcgggca gcgatccagc gatggcctgg aatccgtggc
     2041 acggcatgca atgcgccgaa aaaagcaggg atcctgcctg aagctgctgg ggcgtcgctt
     2101 tgaaagcaag agcagggacc ggcggatcct ggtgaccgaa agcgcgcagt ttcagcctgg
     2161 cagtgccacc aagggcgaat gtgacgatgc gtccatagcc gaatttctcc ggccccccgc
     2221 cgggaaaatg atacagtccg ggcgagccgc ccataccaac catcactgaa atgtattgca
     2281 cgccgtcgac ggtgtaggtg accggcgggg ccatgatccc cgtgccggca tcgaacgacc
     2341 acagttgttt gccgtcggtc gcgcgatacg ccgcgatgat tccgtccgag cgtccctgaa
     2401 aaaccagatt gccggctgtc gccagcgttc cgccgccttc gagcacggga attgccgcgc
     2461 gccaggccgc tttgcgcgca atcggatccc acgccagcaa ttcgccggtc gcgggcacca
     2521 tgtgagccag cttggcgtgc aatggtcctt catgttggtc caagccgatg ttctgagtgt
     2581 tgggatcgta tttccacttg ggatcgggaa cgtgcaccat ttgcgtacca attttggcgg
     2641 cgagatacac cagcccggtt gtcggactga acgcaatcgg gttccagtta tgcgcgccgc
     2701 ccggcgcggg tgacaccatt actggtccgg cgcccgccga tccagctacc tcgatgggcc
     2761 gtcctgtttt tgggtcgagc ccagtggccc acgtgatgcc ggagacgtag ggggttccgg
     2821 aaagaaattc tcccgtggca cggtcaagaa cgtagaagaa accgttcttg ttcggctgca
     2881 tgataacttt gcgcgatcgg ccgccgatgt ttagctcggc ctgcagcaaa ggctgcgttg
     2941 cgtcgtaatc ccagctgtcg cccggtgaag tttggaagtg ccaggcgagt tcaccagtgc
     3001 cggcgtgaag cgcgacgatc gaagcggtgt acagattgtc gccgccgccg cgtagcgcgc
     3061 gataccaagc ggtggcattg ccggttccga aatacaccaa gtccaaagcg ggatcgtagg
     3121 cgatcgaatc ccaaactgta cctccgccac cgactttcca ccattcgccg cgccacgttt
     3181 tggcggcatc ttccatggac ttggattcaa agccgcgcga gggatctccg ggaacggtat
     3241 agaaacgcca ggccagcttg ccggtttccg cgtcgtacgc ggagatgtat ccgcgcacac
     3301 cattttcggc gccggcgttg ccgatcagga cgcggctccc gacgatgcgc ggcgccccgg
     3361 tgatggagta agccttggca atgtcaaccg tgtccacggt ccagacgggc gcgccagtgc
     3421 gtttgtcgag ggcgatcagt ttgccgtcaa gtgttgcgac gaaaact
//
LOCUS       DASTPM010000501         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_161883, whole genome shotgun sequence.
ACCESSION   DASTPM010000501 DASTPM010000000
VERSION     DASTPM010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161883"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VFR42_08900"
     CDS             <1..959
                     /locus_tag="VFR42_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369314.1"
                     /translation="WRVSSMDELPNEVPAGQTDWREVAFAGIGGEKAYKEWAPSVEKN
                     SLAISEEEAAELAKAQPSQIVEEAARGPEDWSKAINAIFHAPKAVIEPVAPVAPTPVA
                     PVEVAPPPVPAFETASAPPISEAVYSAPVAETEPEAAASEPPPALNSWMAGALSPWEA
                     ELQRASDLSSTWDSAKIAPAAVVEETSVTEAFLDSGLMATAHEQVVEAIEEAKAEQPV
                     ETAPEIESGAPIESLYSPAASEVEKTLDAALHEPEAVASEAVAEPARLPVSNGAAPVA
                     TDMDELVARVLAKMSPDVLQAVTREILKPVVEALVKDELNKK"
     gene            1110..3806
                     /locus_tag="VFR42_08905"
     CDS             1110..3806
                     /locus_tag="VFR42_08905"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU0369315.1"
                     /translation="MAREIAKAYDPQQIEGRWAEFWVREKLFRADPQAPGAPFAIVIP
                     PPNVTGSLHIGHMLNHTEIDILTRWHRMRGFNTLYLPGMDHAGISTQRVVVKRLADQG
                     IDYRKLGREEFEKKVWEWKAESGGRITKQMEQIGDSCDWSREKFTLSPEYSRVVREVF
                     VRLYEEKLIYRDTRLVNWCPECLTVLSDLEVVHEERPGHLWYIRYPVSGSSEFIVVAT
                     TRPETMLGDTAVAVNPDDERYQHLIGKNVLLPLMNRQIRVIGDAMVDREFGTGAVKIT
                     PAHDPNDFEAGRRHRLQEIDVMTDDGKMNANAGAYAGMDRFAARKKIVHDLEAHGLLE
                     KTTDHALSVGLCERSRTVVEPRISTQWFCKMKPLAEPAIAAVERGEILIVPENRREEY
                     FHWMRNIRDWTLSRQLWWGHRIPAWYCADCGEVIVAREAPQKCAKCGAQKLRQDEDVL
                     DTWFSSQLWPFVTLGWPDETLDFKKYYPTSLLITGYDILFFWVVRMIMMGLHFTGKVP
                     FHAVYLHSLVRTAGGEKMSKSKGTGLDPVALNEKYGTDAMRFCLTSMAAPGTDIVLSE
                     DRLFGARAFANKIWNASRFLFVNLDKFEQGGAKLEALAARDIREKAPYTFAGNVPLID
                     AWLFARLADTLEVVKAALETYRFHEAVQSIYQFFWNDFCDWYIEWIKPELNGANRERA
                     TVAWQNLFAAFDAALRLLHPVMPFLTEELWHQLPQRANAKSIALERYPEVRAEWRNGA
                     VLQEFALVQEVITSVRAIRADVKLDPKKRVAAEFSSSDAKVRSVIEANLDGILRLALL
                     TELKVSAEKLPAGGAARSTSLFDVRIIYSETVDVGAECTRLKKEHERLTKSIASKEWQ
                     LADDTFRNRAPEKIIQGLETTLAEQRVELSKTTERLSQLNCG"
     gene            3838..>4583
                     /gene="nadC"
                     /locus_tag="VFR42_08910"
     CDS             3838..>4583
                     /gene="nadC"
                     /locus_tag="VFR42_08910"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU0369316.1"
                     /translation="MNWDSEYVQRLVQTALEEDVGAGDATVLATIPAKAIGKARVLAR
                     EDIVCAGLPMAERVFLTLDPKMQIEQRVQEGAVVLKGDVLLHLNGKARAILTGERASL
                     NFLGRLCGIATLVHQFVEKIAGTRARIRDTRKTTPGLRLLEKYAVKTGGGTNHRIGLY
                     DAILLKENHIALAGGVKAALDQANAYAALHAKPSALTAYEEAIPARATDTVLPIQIEV
                     RDKRELHEALSAGAESVLLDNMTPAEARQCV"
BASE COUNT         1005 a         1233 c         1491 g          854 t
ORIGIN      
        1 cgtggcgcgt gagctccatg gatgagctgc caaacgaagt tcccgcaggc caaaccgatt
       61 ggcgcgaagt ggcgtttgcc ggcatcggcg gcgagaaggc ttacaaagag tgggcgccgt
      121 cggttgagaa gaacagcctc gcgatcagcg aggaggaggc cgcggaactg gcgaaggcgc
      181 agccctcgca gattgtggag gaagcggcgc gcggtccgga agactggtcg aaggcgatta
      241 acgcgatttt tcatgcgccg aaagcggtga tcgaaccggt ggcgcctgta gcaccaactc
      301 ctgtggcgcc cgttgaggtt gcgccaccac cggtaccggc atttgaaacg gcatccgctc
      361 cgccgatttc cgaggcggtg tattctgcgc cggtcgccga aacggaaccg gaagctgcgg
      421 catctgagcc gcctcctgcg ctgaattcgt ggatggcggg agcgctgagt ccctgggaag
      481 ccgagctgca gcgtgcgagc gacctttcgt ctacttggga cagcgcgaag attgcgccgg
      541 cggcagtagt ggaagagacc tcggtcaccg aagcgtttct ggattccggc ctgatggcga
      601 ccgcgcacga gcaggtggtt gaggcgattg aagaagcgaa agccgagcaa ccagtggaga
      661 ctgctccgga gattgaatcg ggcgctccca ttgagagttt gtattctccg gcggcatcgg
      721 aagtcgagaa gacgctcgac gcggctttgc atgaaccgga agcggttgcc agcgaagcgg
      781 tagcagaacc ggcccgccta cctgtttcca acggcgctgc gcccgtagcc acggacatgg
      841 atgaactggt agcgagagtc ctggccaaga tgagccccga cgtattgcag gcggtaacgc
      901 gagaaatcct gaagcccgtc gtcgaagcgc tcgtcaaaga cgaattgaac aaaaagtagt
      961 tcccttttcc aaacccgaca aacctctcca agcaaattgt atttccacga cggttgctgc
     1021 tagtttgacg accctgcttt taagcccgta tcatcgaagt ttacttagct cacgccgaaa
     1081 cccgcataac aacaggagaa tgagcaggca tggcccggga gattgccaag gcatacgacc
     1141 cgcaacagat tgaaggacgt tgggcggaat tttgggtacg ggaaaagctg tttcgcgccg
     1201 atcctcaggc gcccggagct ccttttgcca ttgtgattcc tcccccgaat gtgaccggat
     1261 cgctgcacat cgggcacatg ctgaaccaca cggaaatcga catcctgacg cgttggcacc
     1321 gcatgcgtgg cttcaacacg ctgtatctgc ctggcatgga ccatgcggga atttcgacgc
     1381 agcgcgtggt ggtgaagaga cttgccgatc aaggcatcga ctaccggaag ctcggccgcg
     1441 aagaattcga gaaaaaagtc tgggagtgga aagcggaaag cggcggccgc attaccaagc
     1501 agatggagca gatcggcgac agctgcgact ggtcgcggga gaaatttacg ctgtcgccgg
     1561 aatactcgcg ggtggtgcgc gaagtattcg tgcggctgta cgaagagaaa ctgatttatc
     1621 gcgacacccg gctggtgaac tggtgcccgg aatgcctgac agttttgagc gacctggagg
     1681 tcgttcacga agagcggccc ggccatctgt ggtacatccg ctacccggtg tcgggaagtt
     1741 cagaatttat cgtggtggcg acgacgcgcc cggagacgat gctcggcgac acggcggtag
     1801 cagtgaaccc cgacgacgag cgctatcaac atctgatcgg caagaacgtg ctcttgccac
     1861 tgatgaaccg gcagattcgt gtcatcggcg acgccatggt ggaccgcgaa ttcggcaccg
     1921 gcgcggtgaa gattacgccg gcgcacgacc ccaacgattt tgaggcagga cggcggcatc
     1981 gattgcagga aattgacgtg atgaccgacg acggcaaaat gaacgcgaac gctggcgcgt
     2041 atgcgggaat ggaccgcttc gcggcgcgca agaagatcgt gcacgatctg gaagcgcacg
     2101 gcctgctgga aaagaccacc gaccacgcgc tttccgtcgg gttgtgcgag cggagccgga
     2161 cagttgtgga gccgcgcatt tcgacgcaat ggttctgcaa gatgaagccg ctggcggagc
     2221 cggcgattgc ggcggtggag cgcggagaaa tcctgatcgt gccggaaaac cggcgcgaag
     2281 aatatttcca ctggatgcgc aacatccgcg actggacgtt gtcgcggcag ctttggtggg
     2341 gccatcggat tccggcctgg tattgcgcgg actgcggcga agtgatcgtg gcgcgggaag
     2401 ctccgcagaa atgcgcgaaa tgcggcgcgc agaaattgcg gcaggatgaa gatgtgctgg
     2461 acacgtggtt cagctcgcag ttgtggccgt ttgtgacgct gggctggccg gacgaaacgc
     2521 tggacttcaa gaaatactat ccgacctcgc tgctcatcac cggatacgac attttgttct
     2581 tctgggtggt gcggatgatc atgatgggcc tgcactttac aggcaaagtt ccctttcacg
     2641 ccgtctatct gcattcgctg gtgcgcacgg ccggcggcga aaagatgtcc aagtccaagg
     2701 gaaccggact ggacccggtg gcgttgaacg aaaaatacgg cacggacgcc atgcggttct
     2761 gcttgacctc catggctgcg ccgggaaccg acattgtttt gtcggaagac cggttgttcg
     2821 gcgcgcgcgc gttcgccaac aagatctgga acgcgtcgcg attcctgttt gtgaatctgg
     2881 acaaatttga gcaaggcggc gcgaagctgg aagcgctggc cgcgcgggat atacgcgaga
     2941 aggcgcctta taccttcgct ggtaatgtgc cgttgatcga cgcgtggctg tttgcgcggc
     3001 tggccgacac gctggaagtg gtcaaggctg cgttggagac ttatcgcttc cacgaagctg
     3061 tgcaaagcat ttaccagttt ttctggaacg atttctgcga ctggtatatc gagtggatca
     3121 agccggagct gaacggagcg aaccgcgaac gggcgacggt cgcgtggcaa aacctgttcg
     3181 ccgcatttga tgcggcattg cgcctgctgc acccggtaat gccgtttctg acggaagaac
     3241 tgtggcacca attgccgcag cgtgccaatg caaagtctat cgcgctcgaa cgctatcccg
     3301 aggttcgagc cgaatggcgg aacggcgcgg tgctgcagga attcgcactg gtgcaggaag
     3361 tcatcacctc ggtgcgcgcg attcgcgccg acgtgaagct cgatccaaag aaacgtgttg
     3421 ccgcggagtt ttccagcagc gacgcgaagg ttcgctctgt gattgaagcg aacttggacg
     3481 gcattctgcg cctcgcgctg ctgaccgagc tcaaagtttc ggccgagaaa ctgcctgccg
     3541 gcggagcagc gcgctcgacc tctctattcg atgtgcggat tatctatagc gaaaccgtgg
     3601 acgtaggtgc ggagtgcact cgcctgaaaa aagagcacga acgcctgacg aaaagcattg
     3661 cgtcgaaaga atggcaactg gccgacgaca cattccgcaa tcgcgcgccg gagaaaatca
     3721 ttcaggggct ggaaacaacc ctggccgaac agcgggtcga acttagcaag acgaccgaac
     3781 gcctttccca gttgaattgc ggctgaaaac cgcgctagtg tgtcccgcgg cagatccatg
     3841 aactgggatt ccgaatacgt gcagcgattg gtgcagaccg cgctggagga agacgtcggc
     3901 gcgggcgatg ccacggtttt ggcgaccatt cccgcgaaag cgatcgggaa ggcgcgcgtc
     3961 ctggcgcgtg aggacatcgt atgcgcggga ttgccgatgg ccgaacgtgt tttcctcacg
     4021 ttggatccga agatgcagat tgagcagcgc gtccaggaag gtgctgtggt tctcaaggga
     4081 gatgtgctgc tgcatctgaa cggaaaagcg cgagcgattt tgaccggaga gcgcgcctcg
     4141 ttgaattttc tgggacggct ctgcgggatc gccactctgg ttcaccaatt cgtggagaag
     4201 atcgcgggga cgcgggcgcg gattcgcgat acccgaaaga ctacgccggg gctgcggttg
     4261 ctggaaaaat acgcagtgaa aaccggcggc ggaacgaatc atcggatagg tttgtatgat
     4321 gcaattctgc tgaaagaaaa ccacatcgcg ctagccggcg gtgtgaaagc ggcactggac
     4381 caggcgaatg catatgccgc tctgcacgcg aagccgagtg ctcttacggc gtacgaagaa
     4441 gcaattcccg cgcgcgctac ggacacggtg ctgccaatcc agatcgaggt gcgcgacaag
     4501 cgcgaattgc acgaagcgct ctccgcaggc gcggagtccg tcttgctgga caatatgacg
     4561 cctgcggaag ccaggcaatg cgt
//
LOCUS       DASTPM010000502         3070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_882947, whole genome shotgun sequence.
ACCESSION   DASTPM010000502 DASTPM010000000
VERSION     DASTPM010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882947"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1077)
                     /locus_tag="VFR42_08915"
     CDS             complement(157..1077)
                     /locus_tag="VFR42_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369317.1"
                     /translation="MARHLLRRFLLTLPALWLVLTLVFLMIHIVPGDPVEQMLGEGAA
                     PGAVAQLRHALGLDLPLRVQYGHYLSGLVHGDLGQSFKFQAPVARIIFERYPATLQLA
                     FLALVVCAAIAIPAGVLAAHRRGHAADRLVGVFTLIGLAIPNFALGPLLILLFSVGIG
                     ILPVSGRGGAVSYVLPAATLGAALAAILTRMVRGAMLEELSADYIRTARAKGLGTAAV
                     LFRHGLRNALIPIITILGLQFGTLLAGTIVTETIFSWPGVGRLTVQAISARDYPLLQG
                     CILVISLSYVLVNLLTDVVYSLVDPRVRMS"
     gene            complement(1088..2485)
                     /locus_tag="VFR42_08920"
     CDS             complement(1088..2485)
                     /locus_tag="VFR42_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0369318.1"
                     /translation="MFAVKLIGVVLLAALNAFFAATEFSLVAVRLSRVRQLVARGNAR
                     AKIVEALVSDLNRVVSGVQFGITLASLALGALGEATLASVFQSIWPGDPGTKSALVAH
                     AFALTCAFALLTALHVVVGELVPKTISLARAERIALLIARPFRWFLSTFRWAIDLLDG
                     ISGVIIRALGIKAQSGHGVAHSTEELQIQIQQARERGLLAAGEEKFILSAIEMGQVQV
                     REIMVPRPDMHVLAVEATLDEVLRVFATTQKSRIPVYRGTVDHVLGFVHIKDMLWVLQ
                     DRERRAQEGVPVPAFDLRRVLREVLIVPETKPAGELLHEFKKHHTGMAVVVDEFGSLL
                     GLVTLEDVLEQMVGEIHDEFDVVERPLTLADGAVIFDAALNVRDLDAQYGIVLPEDPA
                     YATVGGFVLDQLGFIPKGGESFVFGDLRFTIAEMDGKRVARVKVERIARAGEGTKGGV
                     ETSPRKTTAPAISKS"
     gene            2676..>3070
                     /locus_tag="VFR42_08925"
     CDS             2676..>3070
                     /locus_tag="VFR42_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU0369319.1"
                     /translation="MLRIVFRIIGWLLTLLILVCGGFAWWLFFRPLPQIDGTASLPGL
                     QNPVTVDRDSWGIPHVRASSLADMAEAQGYVMAQDRLWQMDLLRRVARGQLSEILGSA
                     TVPIDKHFRVVRFGPAAERDLTLLDEESRT"
BASE COUNT          655 a         1134 c          684 g          597 t
ORIGIN      
        1 gcccttccag cctcgtcgcc aaactctgcg ccgccggatc cgccggcgct atccacggcg
       61 caaacaccgc caccacaaaa agtagtaacg ccgccgcaaa tcctactcgc gccaacacgc
      121 tctgccgcaa aaacgccctg acatttgcag cgccgctcac gacatcctca cccgcggatc
      181 caccagcgaa tacaccacgt cggtcagcag attcaccagc acatacgaaa gtgaaatcac
      241 cagaatgcag ccctgcagga gcggataatc acgcgccgaa attgcttgta ccgtcaacct
      301 tcccacgcca ggccaggaaa aaatcgtctc cgttacaatt gtccctgcca gcagcgtccc
      361 aaattgcagt cctaagatcg tgatgatcgg aatcagcgca ttgcgcaatc cgtgccggaa
      421 caacacagcc gccgtcccca gtcctttcgc ccgcgcggtt cgaatgtaat ccgccgatag
      481 ttcctccagc attgccccgc gaaccattcg cgtcaaaatc gccgccaggg ctgcccctag
      541 cgtcgccgca ggcaatacat agctgactgc tccgccgcga cccgaaaccg gcaaaatccc
      601 aatcccaacc gaaaagagca agatcagcaa cggccccagc gcaaaatttg gaatcgccag
      661 accaatcaac gtgaacacgc ctaccaatcg gtccgccgca tgacctctgc gatgcgccgc
      721 cagtactccc gcgggaattg caatcgccgc gcacaccacc agcgccagaa acgccagttg
      781 cagtgtcgct ggataccgct caaaaataat tcgcgctact ggcgcctgaa atttgaacga
      841 ctgccccagg tcgccgtgta ccaacccgct caaataatgt ccgtattgca cgcgcagcgg
      901 cagatccagt cccagcgcgt ggcgcaactg tgccaccgcc cccggggccg cgccctcgcc
      961 cagcatctgc tccaccgggt cgcccggcac aatatgaatc atcaggaata ccagcgttag
     1021 taccagccac aacgctggca aggtcagcag gaatcgcctg agcagatgtc gcgccatcgc
     1081 aacaaaatta tgacttggaa atcgccggcg ccgtcgtctt tcgtggcgaa gtttccacgc
     1141 cgccttttgt cccttcaccc gctcgcgcga ttcgctccac cttcacccgc gccacgcgct
     1201 tcccgtccat ctccgcaatc gtgaagcgta aatccccgaa cacaaaactt tctccaccct
     1261 tcggaataaa tcccagttgg tccagcacaa agccgcccac cgtcgcgtac gccggatctt
     1321 ccggcagcac aattccgtac tgtgcatcca gatcgcgcac attcagcgcc gcatcaaaaa
     1381 tcaccgcgcc atcggccagc gtcagcggcc gctccaccac atcgaactcg tcgtgaatct
     1441 ctcccaccat ttgttccagc acatcttcca gcgtcaccaa ccccagcaaa cttccaaact
     1501 catccaccac caccgccatt cctgtatggt gtttcttgaa ctcgtgcaac aactcccccg
     1561 ccggcttcgt ttccggcaca atcagcactt ctctcagcac tcgccttaaa tcaaatgccg
     1621 gcaccggtac accctcttgc gcacgccgct cgcggtcctg tagcacccac aacatgtcct
     1681 ttatgtgtac gaaccccagc acatggtcta ccgtcccgcg atacaccgga atccgtgatt
     1741 tctgggtcgt cgcaaacact cgcagcactt cgtccagcgt tgcttccacc gccagcacat
     1801 gcatgtccgg ccgcggcacc ataatttccc gcacctgcac ttgccccatc tcgatcgcgc
     1861 tcaagataaa tttctcttct cccgccgcca gcagcccgcg ctctctcgct tgctgaatct
     1921 ggatctgcaa ctcctccgtt gaatgtgcca cgccatgccc cgattgtgcc ttgattccca
     1981 gcgctcgaat aattacgccg gagattccgt ccagcagatc aatcgcccac cggaacgtac
     2041 tcaaaaacca acggaacggc cgcgcgatca gcaacgcgat gcgctccgcc cgcgccagac
     2101 tgatcgtttt cggcaccagc tcgccaacta ccacgtgcag cgccgtcagc agcgcgaagg
     2161 cacacgttaa cgcgaacgca tgcgccacca gcgccgactt cgtcccggga tctcctggcc
     2221 atatcgactg gaataccgaa gccagcgtcg cctcgcccag cgctcccagc gccaggctcg
     2281 ccagcgtaat tccaaactgc acgcccgaca ctacgcgatt caggtcgctg accagcgcct
     2341 cgacaatttt cgcccgcgca ttgccgcgcg ccaccagttg ccgcacccgc gacaatcgca
     2401 ccgcgaccag cgaaaattcc gtcgctgcga aaaatgcgtt cagcgccgcc agcaacacca
     2461 ctccgatcag ctttaccgca aacatcttct gcgctctctc cttgccacca gcttaaggac
     2521 ttcagtttgt ctaaccagcc ctgggcgaaa ttgtatcagc acggcgcgga aacgcttctg
     2581 cgcgttttgc cttacactgc aagcatgcaa tcgctgggtt gcgcccctga tacgcctcct
     2641 ctacactgat ttttcactct ttttctgaag ggctcttgtt gcgaattgta ttccggatca
     2701 tcggctggct tctgactctt ctcattctcg tttgcggcgg tttcgcctgg tggctctttt
     2761 ttcgcccgct tcctcaaatc gacggcaccg cttctcttcc cggtctgcaa aatccggtta
     2821 ccgtcgatcg cgatagctgg ggcattccgc atgtccgcgc atcttcgcta gccgacatgg
     2881 ccgaagccca gggctacgtc atggcccagg atcgtctctg gcaaatggac ttgctccgcc
     2941 gcgtcgctcg cggtcagctc tctgagatac ttggctccgc caccgtcccc attgacaaac
     3001 atttccgcgt tgtccgcttc ggccccgccg ccgaacgcga cctcactctc ctggacgaag
     3061 aatcccgcac
//
LOCUS       DASTPM010000503         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_710214, whole genome shotgun sequence.
ACCESSION   DASTPM010000503 DASTPM010000000
VERSION     DASTPM010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710214"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VFR42_08930"
     CDS             <1..247
                     /locus_tag="VFR42_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369320.1"
                     /translation="ALAAGAESVLLDNMPPAEAKQCVAIARSLQPECIVEISGGITLE
                     NVRAYAETGADFLSSGSLTHSAPAANLSLLVDSIEDK"
     gene            263..1267
                     /locus_tag="VFR42_08935"
     CDS             263..1267
                     /locus_tag="VFR42_08935"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0369321.1"
                     /translation="MASTSLPGATDKRLASLVTLFANNATIVISGARIAREIGVSRST
                     VWRWIEQLREVGVRVKGRPHTGYFLERVPDVLTPELLRARLKSSLFGKRICHFFRADS
                     TNRVGMELGYAGEPEGAVILAEEQSAGRGRAGRSWHSERAAGIYATLLLRPKISPMQA
                     PLLTMMAGLSAHTAIQAQTGLQLDLKWPNDLMLNGKKLGGILTEMHADTSLVRFVIVG
                     IGINVNQEKFPGELNTIATSLHAETGKNQSRIELLARLLREFESDYNRFLREGAASVT
                     ERFTKASSYAVGKKVRVTNGAESFTGVTAGLAPEGLLQVAREDGKTVMVIAGDVAEIK
                     "
     gene            1268..1492
                     /locus_tag="VFR42_08940"
     CDS             1268..1492
                     /locus_tag="VFR42_08940"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU0369322.1"
                     /translation="MPATMLLTIDVGNTNTVLGVFRDEELIAHWRLTTARMQTVDEYG
                     VLIRNLFTLKDLDPREVRGVIVSSVVPPMN"
     gene            1495..2085
                     /locus_tag="VFR42_08945"
     CDS             1495..2085
                     /locus_tag="VFR42_08945"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU0369323.1"
                     /translation="MAQTSKLYFEQKAIFVEPGVKTGMSVLVDNPQEVGADRIVNGVA
                     AFHKYGGPCIVVDFGTAITFDVISAKGEYQGGVIAPGIGIASEALFARAAKLPRVEIK
                     DPGKVIGSNTVANMQAGLYYGAVDMVDGMLTRMKAELGGKVKVVATGGQAKLVAKGSK
                     HIDETDEFLTLEGLRLIWEKNQSNDASKKTERKAAR"
     gene            2093..>2334
                     /locus_tag="VFR42_08950"
     CDS             2093..>2334
                     /locus_tag="VFR42_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369324.1"
                     /translation="MDGWNYFNYFTEIEEYFWKKRGSHLLVSPLDWAIMETWQKAGVP
                     LEAVFKGIDSAFESYGRSRRGAGKPLKSLAYCTDAVL"
BASE COUNT          514 a          635 c          735 g          450 t
ORIGIN      
        1 agcgctcgcg gccggcgcgg agtcggtctt gctggacaac atgccgccag ccgaagccaa
       61 gcaatgcgtg gcgatcgcgc gcagcttgca gccggaatgc atcgtggaga tttccggcgg
      121 catcacgctc gagaacgttc gcgcctacgc ggagacaggc gccgattttc tatcctcggg
      181 ctccctcacc cactccgctc ccgcagccaa cctgagcttg cttgtggact ccatcgagga
      241 caagtaagct accgctacat ttatggcttc tacttccctg ccgggcgcta ccgacaaaag
      301 gctcgcgtcc ctcgtcactc tctttgcaaa caatgccacg attgtaatca gcggcgcgcg
      361 cattgcgcgt gaaattggcg tgtcgcgttc gaccgtgtgg cgctggatcg agcagctgcg
      421 cgaagttggc gttcgcgtga agggccgccc tcataccgga tattttctgg agcgcgtgcc
      481 ggacgtcctg acgccggagc ttttgcgggc caggctgaag agcagtctgt tcggaaagag
      541 gatttgccat tttttccgcg ccgattcgac gaaccgcgtc ggaatggaac ttggctacgc
      601 tggcgaaccg gaaggcgcgg tgatcctagc tgaagaacaa agtgcaggcc gcggccgagc
      661 ggggcgaagc tggcactccg agcgcgcggc cggcatctat gcgacattac tgctgcggcc
      721 aaaaatctcg ccgatgcaag cgccgctgct caccatgatg gccggacttt ccgcacacac
      781 ggccatacag gcacagaccg gattgcaact cgacctgaag tggcccaacg atctgatgct
      841 gaacggcaag aagctgggag gaatcctgac cgaaatgcat gccgacacat cactggttcg
      901 ttttgtgatt gtggggatcg gcatcaacgt caaccaggag aagtttccgg gagagctgaa
      961 cacgatcgcc acgtcgttgc acgcggaaac cggcaaaaac caatcccgca tcgaactgct
     1021 agcgcgtttg ctccgcgaat ttgagagcga ttacaatcgt ttcttgcgcg aaggcgcggc
     1081 ttccgtcacc gagcgcttca ccaaggcttc gtcgtacgca gtgggaaaaa aggtgcgtgt
     1141 gacgaatgga gcggagagct ttaccggcgt gacggccggc cttgcacccg aaggcctgtt
     1201 gcaggttgca cgcgaggacg gcaagaccgt tatggtgatt gccggcgatg ttgcggaaat
     1261 caagtgagtg ccagccacga tgctgctgac cattgacgta ggcaatacca acaccgtact
     1321 gggggtattc cgcgacgaag aactcatcgc gcactggcgg ctaactaccg cgcgcatgca
     1381 gacggtggac gaatacggcg tgctgatccg caatctgttt acgctgaaag atctcgatcc
     1441 gcgcgaagtg cgaggcgtta tcgtgagttc ggtggtgccg ccgatgaact gactttggcg
     1501 caaacgtcga aactttattt cgaacagaag gcgatctttg tcgaacccgg cgtgaagacc
     1561 ggaatgtccg tcctggtgga caatccgcag gaagtgggcg ccgaccgtat cgtcaacggc
     1621 gtggcggcgt ttcataagta cggcgggccg tgcattgtgg tggattttgg cacggcgata
     1681 acgttcgatg tgatttcggc aaagggcgag taccagggcg gagtgattgc tcccggaatt
     1741 ggcattgcgt cggaggcatt gttcgcgcgt gcggcaaaac tgccccgagt cgaaatcaag
     1801 gatcccggca aggtgatcgg cagcaatacg gtcgccaaca tgcaggcagg cctgtactac
     1861 ggcgcagtag atatggtgga cggcatgctt acgcgcatga aagcagagct gggcggtaaa
     1921 gtgaaagttg ttgcgacggg cggacaagca aaactcgtgg ccaaaggctc caagcacatc
     1981 gatgaaacgg atgagtttct gacgctggaa ggcttgcggc ttatctggga gaagaaccag
     2041 tccaacgacg ccagcaagaa aaccgaacgc aaagcggccc ggtagatcgg gcatggacgg
     2101 ctggaactac ttcaactatt tcacggaaat cgaagaatat ttctggaaga aacgcggctc
     2161 gcatctgctg gtctctccgt tggattgggc catcatggag acttggcaaa aagcgggcgt
     2221 tcctctggaa gccgtgttca aggggattga cagcgcgttt gagagttatg gccggtcgcg
     2281 gcgaggggcc ggcaaacccc tcaagagcct ggcgtactgc acggacgcgg tgct
//
LOCUS       DASTPM010000504         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_969418, whole genome shotgun sequence.
ACCESSION   DASTPM010000504 DASTPM010000000
VERSION     DASTPM010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969418"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1106)
                     /locus_tag="VFR42_08955"
     CDS             complement(222..1106)
                     /locus_tag="VFR42_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0369325.1"
                     /translation="MLQKEEACMAPVRYRTADVDGFKIFYREAGPADAPRLLLLHGFP
                     SAGHMFRDLIPLLADRFRMVAPDLPGFGQSDMPPREKFRYTFDNIADVIDRFTEVIGF
                     DRFAVYVFDYGAPTGFRLAVRHPERITAIISQNGNAYEEGLSEGWNPIRAYWQDRSAE
                     NRNGLRAFLAPETTRWQYTHGVTDETAVSPDGYSLDNFYLARPGADEIQLDLFGDYKS
                     NIALYPAFQDYFRTHKPPFLAVWGKNDPFFLPPGAEAFRRDIPDAVVRFFDTGHFALE
                     THVREIADEIRAFLISRN"
     gene            complement(1254..1604)
                     /locus_tag="VFR42_08960"
     CDS             complement(1254..1604)
                     /locus_tag="VFR42_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369326.1"
                     /translation="MERTLNALGVRRPVEVSQNAPTKSPQHYDLVRPRPPVSSASVKQ
                     MNSENWKEIELNYCGARVKGSYKVSEKLVTVVYGSGIKTAQLGILPAEMLAHMLLREL
                     EFERRETKRQTLGE"
     gene            2012..>2218
                     /locus_tag="VFR42_08965"
     CDS             2012..>2218
                     /locus_tag="VFR42_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369327.1"
                     /translation="MAACCQRAASPVHLLKKPKISAMTANHKHDNPILVTGAAGAVGG
                     VGRNRIPARKGHKVRALLRREDERA"
BASE COUNT          476 a          646 c          613 g          483 t
ORIGIN      
        1 ctttaaacgc gacattcccg atgcgatcgt tcgtttcttc gacaccggcc acttcgcgct
       61 ggaaacacat gccgaggaaa tcaccaaagc gatccaggac ttccttgttc actgagtcag
      121 cgcgggccga ccttacgtca tcgagcgagg aaaccgaaca aaataaggcc caacctcctg
      181 tctgatcttt gcaaatccgt catgcccggc catgacggcg ttcagtttcg gctgatgaga
      241 aaggcgcgaa tctcatccgc gatctcgcgc acatgcgttt ccagcgcgaa gtggccggtg
      301 tcgaagaaac gtaccactgc atccggaatg tcgcgcctga aggcttccgc gcctggcggc
      361 agaaagaacg gatcgttctt gccccacacc gccaggaacg gcggcttgtg ggtacggaag
      421 taatcctgga aagccgggta gagcgcgatg ttgctcttgt agtcgccaaa cagatcgagc
      481 tggatttcat cggcgcccgg ccgcgccaga tagaaattgt cgagcgaata gccatccggc
      541 gacacggccg tctcgtccgt gaccccgtgc gtatattgcc agcgcgtggt ttccggcgcg
      601 aggaaggcgc gcaagccgtt tcgattttcg gccgagcggt cctgccaata ggcgcggatc
      661 ggattccagc cttcgctcaa gccttcttcg taggcattgc cgttttgcga aatgatcgcg
      721 gttatccgct caggatggcg gaccgcgagc cggaagcctg ttggtgcgcc gtagtcaaag
      781 acgtacaccg caaaccgatc gaagccgatg acctcggtaa agcggtcgat cacatcggcg
      841 atgttgtcga atgtgtatct gaatttttct cgcggcggca tatcggattg accgaaccca
      901 ggaaggtccg gggcgaccat acgaaatctg tcggcgagca gcgggatcag gtcacgaaac
      961 atgtggcccg cgctcgggaa gccgtgcagc aacagcagcc tcggcgcgtc ggctggcccc
     1021 gcttcgcgat agaagatctt gaaaccatcg acatcggccg tgcgatatct gaccggcgcc
     1081 atacaagcct cctctttttg caacatccct gacggcccaa tcccatcagt tgcctgacat
     1141 cgcggccctg catggacgac tttgccgcga tacttatggt tgaccccggg ttgcagcggg
     1201 cgccttcgga tttttgagag tccggaaagg gtgccaccgc tgcgaaagaa agatcactcg
     1261 cctagagtct gccgtttcgt ctctcgtcgc tcgaattcca attcacgcag cagcatgtga
     1321 gccaacatct cagcaggcaa aatacctagc tgcgctgttt tgatgccgct cccatacaca
     1381 acagtcacca gtttttcgga gaccttgtag ctgcccttaa cccgcgcccc gcagtaatta
     1441 agctcgatct ctttccaatt ttcggaattc atttgcttca ccgacgcgga tgaaaccggc
     1501 ggccgaggtc gcaccaaatc gtaatgctgt ggggacttgg tgggcgcatt ttgagacact
     1561 tcaacaggtc tccgaacgcc taaggcattc aatgtacgtt ccattatttt tctcagagct
     1621 ggctccctct ggacacccag gcaggaagga gatgttcgaa acgctaaacg tccggcagca
     1681 ccccaagttt caggcggttt agttcgaaat tttgaccgtc gtttgtcttt tttaaatagg
     1741 gtggcgtggc cctgtgtcag gatttgtccg atgagaccaa tggcttcccg tgaaattccc
     1801 tatgcaacag aacagggaat ttttttgaaa gaacaggtga agggttcact tccgcttttg
     1861 acccaaagct gacattgacc ttgatcacag ttgtgtgccg tttctcactc gccgcccggt
     1921 cgtaaagtgg taggctttgg acactcagca aggcgtgttc tcagaggaca catgcagcga
     1981 cgcgagttca tcacgcttct ccagcagttg catggccgct tgctgccagc gcgccgcgtc
     2041 gccagttcat cttttgaaga aacccaagat cagcgccatg acggcgaacc ataaacacga
     2101 caatccgatc ctcgtgaccg gagcagccgg cgctgtgggc ggcgtcggcc gcaaccgaat
     2161 tcctgctcgc aaggggcaca aggtgcgcgc cttgcttcgg cgtgaggacg agcgcgcc
//
LOCUS       DASTPM010000505         6184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_46965, whole genome shotgun sequence.
ACCESSION   DASTPM010000505 DASTPM010000000
VERSION     DASTPM010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46965"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /gene="amrB"
                     /locus_tag="VFR42_08970"
     CDS             <1..263
                     /gene="amrB"
                     /locus_tag="VFR42_08970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEU0369328.1"
                     /translation="HYENQSATVRKDNLAIEKILALDPAGLYEICRKEKISMCGLGPT
                     VAMLSALRHAGVSRADLVRQATSADYSGDTRKVVGYAGFLFS"
     gene            complement(352..2049)
                     /locus_tag="VFR42_08975"
     CDS             complement(352..2049)
                     /locus_tag="VFR42_08975"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEU0369329.1"
                     /translation="MSGSANADTSHARIVRLSLVQGDVRFARDVKGDPLNDSSQLNWE
                     SAALNLPIRQGYVVATDNGRVEVEFENGALVFLAENTVLQFYDLSSEDGGLTTRLILR
                     QGSAEFWVNPARGDYFSVTGGDFSVEAESKATFRMNNFDDGSNVSVLRGHLTALANGK
                     NTALAKGDEFSMRAGEADSGAVARAGESDEFDQWAAGRISTAASATNASLPLTNGSGY
                     VSGYGDLYTYGAWSPVAGYGSCWRPFGVGLGWSPFDYGSWYYDPFFGWAFLGGYSWGW
                     LPYHYGGWLFQPGVGWVWSPAGVQGSPGVGRWRPVTGVWMRASNGGTAIVPAHPLDGR
                     GKTPMNLSQGMFAVTTRGVTGTVAPQSDARWKVENKPGREVIQNQLATAAAPTRVSRS
                     MAASTEAGGRGAAVPGGSTIVFDRSEGRFVNSTKARANEPAAATAGASNGTAEQAQRG
                     MKVSPAQDRSGNGARVPPASDRMNGRETAGRQVTPATRTAPPPPSVPRSVTTERVFNQ
                     MNSSGSGGGRAGGGAGASGASSGGAGRGSAPSGGASSGGGRSSGGGSSGGGGGGRPH"
     gene            2556..6143
                     /locus_tag="VFR42_08980"
     CDS             2556..6143
                     /locus_tag="VFR42_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0369330.1"
                     /translation="MHRSLQVLLGVFLLAVLSLTTVSTAHAQGGATGAISGVVLDSSG
                     SSVADAEIQIINSATEVLVRKLPTAADGSFVATLLPPGTYYAVVNKSGFSQAKAEGIE
                     VRVTETTKVTISVKPGSVAEKVEITADVTSVETTNATTGQSIGSNAVRTLPLATQNFQ
                     QLLTLSAGAQSELNSSTGLGRGDVRIIVNGQREDNNNYLIEGISATDYNVAQLTNTPL
                     PNPDVIQEFKVQTSLYDATQGRNGGGNVNAILKSGGRQFHGDAYEFFRNDVLNANEYF
                     LNASGQPRPVLKQNIFGGSLGGPVGTEKAGFFFVNYQGTRQRSGDAPGTVISTRIPAI
                     PLDRSEGALISTFFPLGLPTGVTGLDPVALALLNFKSNQFGGASNGYLIPSVADDGTG
                     FGTFSFSSPGKFTDDQFTANWDREFRGGGDKISFRFFYSNSETFEPFGAGGLQASLGG
                     SIAASDLNFPYTLPVRSRFLNFTETHLFSPTVVNEVRFGFNHINNSAINNDPVTVGDL
                     GIDRPTNNLTSSIYKFTLGSSGFQIGPTPQSNTANEQNNYTFLDTVSWVHGSHVVRFG
                     GEVNFVNLDKLFPQVFNGQLFFVNDLNAGSTANALTDWQRLLVGAPDFSFGGGGVFNH
                     KYRQNNFALFAQDDWKATRNLTLNLGLRTEFLGAWTDGACHIGNVQSSLTLQDQNPLV
                     YPSCVNSLDVPGLIGSADNSTFNNRYSTGLGPRIGLAYDVFGHHTTTVRAGYGIYFVR
                     EDVGAVDQMSFQSPFLPIAFGGGGPGSLGSFFLTPPNALPPAGTLDPTFIPVFSHLQG
                     FVDHLGNATTDTTQTPVYDGSTVGLFMLEVPRNFKVPNTQQWNVTVQRSLGKSWVLEV
                     GYVGTHAIHLRETRDGIQSLNATPASPVVVTLADGSRLPITANTFSNAIARTPTPGLN
                     GYSGYQLFANDAYSHYHSLQTTLSRRSARGYFQAAYTWSRSTDATSTGNTAFNTAYND
                     QSDINASRGLSDFDRPHRFVVSYVYDLPFFSKSTGFKRAVLGGWGVSGVTVVQSGLPF
                     SIFDSGAATAFLGAGSTPLLGASLASGTAISNGYTHGDIHSRLDGFVDINNFTTAPLL
                     YPALCDPTQTDPSVTPNSNYCVTGFGDLGRNIYRGPYQQNWDFSLIKNFHLTERQNIR
                     FTTDFFNIWNHANFSNPVSTDVENPGSFGFITSTKGVPRLIQFSLRYSF"
BASE COUNT         1186 a         2175 c         1515 g         1308 t
ORIGIN      
        1 atcactacga aaatcagtct gcgaccgttc gaaaagacaa tcttgccatc gaaaaaatcc
       61 tggcactgga tcctgccggc ctttatgaaa tttgccgcaa ggaaaaaatc tccatgtgcg
      121 gtctgggccc aaccgtggct atgctatccg cactgcgaca tgccggagtt tcaagagccg
      181 acctggtgcg acaagcaacg tccgccgact attccggtga caccagaaaa gtggtcggct
      241 acgcgggctt ccttttttcc tagactacaa accatgaccg cgtttcccca aaaaagaggc
      301 tccttcagga gcctcttttt tcgacactga aaaagttgac gttttccggt gtcaatgcgg
      361 acggccgcca ccaccgcctc cactgcttcc gccacctgag cttcttcctc cgccactcga
      421 tgcccctccc gacggcgcag acccgcgccc cgcgcctcca gatgaggcac cggaagcccc
      481 tgctccgccg ccagcgcgcc cgccgccact gccgctcgaa ttcatttgat tgaatacccg
      541 ctccgtggtc accgagcgcg gaacgctcgg cgggggcggt gcggttcgcg tggccggggt
      601 gacctgccga cccgccgttt ctcttccgtt catccggtcg gaagccggag gcacccgcgc
      661 gccatttccg ctgcgatcct gcgccggcga aactttcatg ccgcgctgcg cctgctccgc
      721 ggtgccgttg ctggccccag cggttgcggc ggcaggttcg ttcgctcgcg ccttcgtcga
      781 attcacgaaa cgtccttcgc tccggtcaaa cacaatcgta gatccgccgg gaaccgccgc
      841 gccgcgcccg ccggcttcgg tgctcgccgc catcgaccgc gaaactcgtg ttggtgccgc
      901 ggctgtcgcc aactgatttt gtatcacctc gcgccccggc ttgttttcca ctttccatct
      961 tgcatcgctc tgcggcgcga ccgttcccgt cacgccgcgc gtggttaccg caaacattcc
     1021 ttgcgaaagg ttcattggcg tcttgcctcg tccatcgaga ggatgagccg gtacaattgc
     1081 cgttccgcca ttcgaggctc gcatccacac gccggtcact ggcctccacc gccctacgcc
     1141 cggacttccc tgcacgccgg ccggactcca cacccagcct actcccggct gaaacagcca
     1201 cccgccgtag tgatagggca gccatcccca ggaatacccg cccaggaatg cccagccaaa
     1261 aaatggatcg taataccacg aaccataatc aaacggtgac cagccgagcc ccactccaaa
     1321 tggccgccag cagcttccat aaccagccac cggagaccac gctccatacg tgtacaaatc
     1381 gccgtagccg gaaacgtagc ccgagccatt cgtcaacggc agtgatgcat tcgtagccga
     1441 cgcggcggtc gagattcgcc cggcggccca ctgatcaaat tcgtcgcttt ctcctgctcg
     1501 cgccactgca cctgaatccg cttctccggc gcgcatcgag aattcatcgc ccttcgccag
     1561 cgccgtgttt tttccgttgg ccagcgccgt caagtgcccg cgcagcacgc tcacattgct
     1621 gccatcatca aaattgttca tccgaaacgt ggctttggac tccgcttcca cgctgaagtc
     1681 gccgcccgtc acgctgaaat aatcgccgcg cgccggattc acccaaaact ccgccgagcc
     1741 ctgccgcagg attagccgcg tcgtcaaccc gccgtcctcg ctcgacaagt cgtaaaactg
     1801 cagcaccgtg ttctccgcca aaaacaccag cgcgccgttc tcaaactcca cttccacgcg
     1861 cccattgtcg gtcgctacca cgtagccctg ccggatcggc aaattcaatg ccgccgactc
     1921 ccagttcaat tgcgacgaat cgttcagcgg atcgcccttc acgtctcgcg caaatcgcac
     1981 atcgccctgc actaggctca gtcgcacaat gcgtgcatga ctggtatcag cgttcgcgga
     2041 cccgctcatg cagcttgcga cgaaaaaccc gaaaagtagg attggaagaa ctctgccacg
     2101 gctggtcatt gctcaccccc tggtggggac cccgtccatt tagtaggacg ccggccggag
     2161 tgccgaggtt acctgcttgc ttttggctcg gaaaaccacg acctgggtgc ttcccatact
     2221 gttctggcgc tctggccagg tcaagtcaaa tgcaagtcaa agaaagcgcc gtttgccgcg
     2281 gttttcatct ggttccgcgc gatcacgggc tcggttttcc caatgcatgg gatggaaatc
     2341 ttgcttgcac ggttccttga cactcggaaa gtgaaacact agtttcagta ttagggggta
     2401 gtgtccgttt gcagctacca acgccttgcg atcctgatgt tgctgcggtc ttccgcatgc
     2461 ttcggggtgg gatcgttcaa ggatttcggg ctgcggggcc tgtttggcgg ttcccgccgc
     2521 tcgacatcga agtccaagtt aggggataaa ccataatgca tcgcagtctg caggtcttgc
     2581 tcggggtgtt tctgctcgca gtgctcagcc ttaccaccgt ttccacggcc cacgctcaag
     2641 gcggcgccac cggcgccatc agcggcgtgg tgctcgactc ctccggctcc tctgtcgcgg
     2701 acgccgaaat ccagatcatc aacagcgcca ccgaagttct ggtccgcaaa cttcctaccg
     2761 ccgccgacgg ctctttcgtc gccaccttgc tcccgcccgg gacttactac gctgtggtca
     2821 acaagtccgg cttttcgcaa gccaaagccg aaggcatcga agtccgcgtc accgaaacca
     2881 ccaaagtcac catctcggtc aagcctgggt cggtcgcgga aaaagtcgaa atcaccgccg
     2941 acgtcaccag cgtggaaact accaatgcca ccaccggcca gtccatcggc tctaacgccg
     3001 tgcgcactct gccgctggcc acgcagaatt tccagcagct cctgaccctc tccgccggcg
     3061 cacagagcga actcaacagt tccactggtc tcggccgcgg cgacgtccgt atcatcgtca
     3121 acggccagcg cgaagacaac aacaactacc tcatcgaagg catctccgcc accgactaca
     3181 acgtcgccca gctcaccaac acgcctctgc ccaatcccga cgtcatccaa gagttcaagg
     3241 ttcagacctc gctctacgac gccacacagg gccgcaacgg cggcggcaac gtcaacgcca
     3301 ttcttaagtc cggcggtcgc cagttccacg gcgatgcata cgaatttttc cgcaatgacg
     3361 ttctcaacgc caacgaatac ttcctcaacg ccagcggcca gcctcgtcca gttctcaagc
     3421 agaatatatt cggcggcagc ctgggcggtc ccgtcggcac ggaaaaagcc ggcttcttct
     3481 tcgtcaacta ccagggcact cgccagcgca gcggcgacgc ccccggcacc gtgattagca
     3541 cccgcattcc agcgattccc ctcgatcgtt ccgagggtgc gttgatctcc acgttcttcc
     3601 cgctcggttt gccgaccggc gttaccggtc ttgatcccgt cgccctcgcg ctgctcaatt
     3661 tcaaaagcaa tcagtttggc ggcgcttcca acggctacct gattcccagc gtcgccgatg
     3721 atggcaccgg cttcggtacc ttcagcttta gctcgcccgg caaatttact gacgatcagt
     3781 tcaccgccaa ctgggaccgc gaattccgcg gcggcggaga caaaatctct ttccgcttct
     3841 tctattccaa ctcggaaact tttgagcctt tcggtgctgg cggcttgcaa gcttctctcg
     3901 gcggcagcat cgcggcttcc gacctcaact tcccctatac cttgccggtg cgcagccgtt
     3961 tcctcaactt tacggaaact cacctctttt ctcccaccgt ggtcaacgaa gtccgctttg
     4021 gcttcaatca catcaacaat tccgccatca acaatgatcc cgtcaccgtc ggtgatttgg
     4081 gcatcgaccg ccccaccaac aatctcacca gcagcattta taagttcacg ctgggcagtt
     4141 ccggtttcca gatcggtccc acgccgcaat ccaacaccgc taacgagcaa aacaactaca
     4201 cgttcctcga caccgtctcc tgggtgcatg gttcacacgt ggttcgcttc ggcggcgaag
     4261 tcaattttgt taatctcgat aagcttttcc cgcaggtctt caacggacag ctgttcttcg
     4321 tcaacgattt aaacgccggc agcaccgcga atgctcttac cgactggcag cgtcttttgg
     4381 tcggcgcgcc cgacttcagc ttcggcggcg gcggcgtctt caatcacaaa taccggcaaa
     4441 acaatttcgc gctgtttgct caagacgact ggaaggccac tcgcaacctc acgttgaatc
     4501 tgggcctccg taccgaattt ctcggcgcct ggaccgatgg cgcctgccac atcggcaacg
     4561 tccaatcctc gctcacgctg caggatcaga atccgctcgt ctatccgagt tgcgtgaaca
     4621 gcctcgatgt gcccggcctc atcggctcgg ctgacaattc cacctttaac aaccgctact
     4681 ccaccggact cggcccgcgc attggtcttg cttacgacgt ctttggacat cacaccacca
     4741 ccgtgcgcgc cggttacggc atctacttcg ttcgcgaaga tgtcggcgcc gtcgatcaga
     4801 tgagcttcca gtcgccgttc cttcccatcg cttttggtgg tggcggccca ggcagcctgg
     4861 gcagtttctt cctgactccg cccaacgcgc tgcctccagc cggcacgctc gatccaacct
     4921 tcatacccgt gttctcccat ttgcaaggct tcgtggacca cctcggcaac gccaccaccg
     4981 acaccacgca aacgccagtt tacgatggct ccaccgttgg cctcttcatg ttggaagtgc
     5041 cgcgcaactt caaagtgccc aacacccagc aatggaacgt caccgtgcag cgttcgctcg
     5101 gcaagagctg ggtcctggaa gtcggctatg tcggtaccca tgccattcac ctgcgcgaaa
     5161 cgcgggatgg cattcaatct ctcaacgcaa caccggccag tccggtagtc gtcacgctgg
     5221 ccgacggatc tcggctgccg attaccgcca acaccttttc caacgccatt gcgcgtactc
     5281 cgacgccggg cctcaacggc tactccggct accagctctt tgccaacgat gcctactcgc
     5341 actatcactc attgcagacc accctctcgc gccgctcggc tcgcggctac ttccaggcag
     5401 cctacacctg gtccagatcc accgacgcca cttccaccgg caacaccgct ttcaacaccg
     5461 cttacaacga tcagagcgac atcaatgctt cccgcggact ttccgatttc gaccgcccgc
     5521 accgtttcgt ggtcagctac gtctatgatt tgcccttctt ctccaagtcc accggcttca
     5581 agcgcgccgt gctgggcgga tggggcgtca gcggtgtgac cgttgtccag tccggcttgc
     5641 ccttctccat tttcgattcc ggcgcggcca cggctttcct cggcgcgggc agcacgccgc
     5701 ttctcggcgc cagcctggct tccggcaccg ccatttccaa cggctacacc catggcgaca
     5761 ttcacagccg cttggacggc ttcgtcgaca tcaacaattt cactaccgcg ccgctgctct
     5821 atccggcgct ttgcgatccc acacagaccg atccttcggt tactccgaat tccaactatt
     5881 gcgtcaccgg cttcggcgac cttggccgca acatttatcg aggtccctat cagcagaact
     5941 gggacttctc gctcatcaag aatttccatt tgaccgagcg ccagaacatc cgtttcacca
     6001 ccgacttctt taacatctgg aaccacgcca acttctccaa cccggtttcc accgatgtgg
     6061 aaaatcccgg cagcttcggc ttcatcacca gcaccaaggg cgtgccgcgt ctgattcaat
     6121 tctcgttgcg ctactccttc tagtctggtg acttatctct gggtatacga gcggctggtt
     6181 cctt
//
LOCUS       DASTPM010000506         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_104450, whole genome shotgun sequence.
ACCESSION   DASTPM010000506 DASTPM010000000
VERSION     DASTPM010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104450"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /locus_tag="VFR42_08985"
     CDS             <1..1742
                     /locus_tag="VFR42_08985"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEU0369331.1"
                     /translation="AAQSVAGQALGEIAASAYDAPLGNAAAAPASVSPSAATDASYDL
                     IIRNGHIIDGAGNPWYNADLAVTGNRIAAIGDLRSAHAKREIDASGQIVAPGFIDMLG
                     QSEFPLLIDNRSLSKLAQGITTEITGEGGSIAPQNEKTVAALKPFADQYKLSIDWTSL
                     DQYARRLEKQGTPLNIGTYVGSAQIREAVIGEDDRAPTPAELQQMQSLVEQAMKDGAL
                     GVSSALIYPPNIYAKTDELIALAKVASKYGGLYATHMRSEGASETAALAEAIRIGREA
                     NLPVEVFHLKVSGKPRWGNMKQVVATIQAARDSGLDIAADMYPYIAGGTALASALPPW
                     VADGGAQKLLARIKDPAIRARIKKELATDHSDWENLLFDCGGGTGVMIASVENPTLKQ
                     WEGKTVDDVAKAWKKSTEDTLMDFVLADNAQTGALYFMASEEDLKTGLSQPWTSIGLD
                     ANEMSLDGLLFEAHGHPRAFGSMPRFLGRYVRDQHLMPLEAAVRKITSLPAQRQHLAD
                     RGLLKPGYFADITIFDAAKIIDHATYEKPAQLAEGVRFTIVNGQIEFDEGKLTGATAG
                     KFLRGRGWNPTTN"
     gene            complement(1920..2303)
                     /locus_tag="VFR42_08990"
     CDS             complement(1920..2303)
                     /locus_tag="VFR42_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369332.1"
                     /translation="MRPRSAVRVSYVIVLSLLGLVLPSQSRAQVLDVSGRYQCAEAKV
                     RGKVVPCTAAPLTLKNDGKFELRGWEGSYLVNGQWVELSDSAVRSRAKIEPGHKIVFR
                     YRGKHGWCEMTYERRVAELGKTSLS"
     gene            complement(2557..>2993)
                     /locus_tag="VFR42_08995"
     CDS             complement(2557..>2993)
                     /locus_tag="VFR42_08995"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0369333.1"
                     /translation="FGMTPGLPNSLAGHKRPLHTIIPAFMQKGDMHIGFGIMGGWNQS
                     QAHAQFIANVADFKMNIQAAMEAPRFSKHSFDGCDVLMENRIPAAARNELSGKGHKIQ
                     VLGMYSSGVGGGQAVLRDFATGVNYGASDPRKDGQASAEVPN"
BASE COUNT          623 a         1073 c          712 g          585 t
ORIGIN      
        1 aagccgcgca gtccgtcgct ggccaggccc tcggcgaaat cgcggcctct gcctacgacg
       61 caccgcttgg caacgctgcc gccgcgccag cctctgtttc ccccagcgcc gcgaccgacg
      121 cctcttacga tctcatcatc cgcaatggac acatcatcga cggtgccggc aacccctggt
      181 acaacgcgga cctggccgtc acgggcaacc gcatcgccgc cattggcgac ctgcgctccg
      241 cccacgccaa acgcgaaatc gatgcctccg gccagattgt cgcacccgga tttatcgaca
      301 tgctcggtca gtccgaattc cctctcttga tcgacaatcg ttccctcagc aaactcgcgc
      361 agggcatcac caccgagatc accggcgaag gtggctccat cgcgccgcaa aacgaaaaaa
      421 ccgttgctgc cttgaagccc tttgccgacc aatacaagct cagcatcgat tggacatcgc
      481 tcgaccagta cgctcgccgc ctcgaaaagc agggcacgcc actgaacatc ggcacctacg
      541 tcggctccgc gcagattcgc gaagccgtca tcggcgaaga cgatcgcgcg ccaactcccg
      601 ccgagcttca gcaaatgcaa tccctcgtcg aacaagccat gaaagatggc gcgctcggcg
      661 tctccagcgc tctcatttat ccgccgaaca tctacgctaa gaccgacgaa ctcatcgcgc
      721 tcgccaaagt cgcctcgaaa tacggcggcc tctacgccac acatatgcgc agcgagggcg
      781 cttctgaaac tgccgctctc gccgaagcca ttcgcatcgg ccgcgaagcc aatcttcctg
      841 tcgaggtctt ccatctcaaa gtcagcggca agccgcgctg gggcaacatg aaacaagtcg
      901 tcgccaccat tcaagccgcc cgcgattctg gtctcgatat cgccgccgac atgtacccgt
      961 acattgccgg tggcaccgcg ttggcatccg ctcttccgcc ttgggttgct gacggcggcg
     1021 cgcaaaagct tctcgcccgt atcaaagatc ccgcgattcg cgcgcgcatc aagaaagagc
     1081 tcgctaccga tcactccgat tgggaaaatc ttcttttcga ttgtggcggc ggcaccggcg
     1141 tgatgatcgc ttccgtggaa aatccgacgc tcaagcagtg ggagggcaag accgtcgacg
     1201 acgtcgccaa ggcctggaaa aagtccaccg aagacacgct gatggatttc gttctcgccg
     1261 ataacgcgca aaccggcgcg ctctatttca tggccagcga ggaagatcta aagaccggcc
     1321 tctcccagcc ctggaccagc atcggcctcg acgccaacga aatgtctctc gacggtctgt
     1381 tgttcgaggc ccacggtcat ccccgcgcat tcggctccat gccccgtttc cttggccgct
     1441 acgttcgcga ccagcacctc atgccgcttg aagccgccgt ccgcaaaatt acctcgttgc
     1501 ccgcgcagcg tcagcatctt gccgatcgcg gcctgctcaa gcccggctat ttcgccgaca
     1561 tcactatctt cgacgccgca aaaattatcg accatgccac ctacgaaaag cccgcacagc
     1621 tcgccgaagg tgtgcgtttc accatcgtca atggtcaaat cgaattcgat gaaggcaagc
     1681 tgacaggcgc cacagccggc aagtttctgc gaggccgcgg ctggaatccc acaacgaact
     1741 gaatgatgaa gagcgtaccc gaagggcaaa ttgcggtgat ctgcggccgg cagcttcggg
     1801 cttcccgacc aagctccaag tacaacaatg tcatctcgag cgaagccgag ggacctgctt
     1861 tttggctttg aactcttaaa ctcttgaact ttcgaacttt taaactcttg aactcttctt
     1921 caactcagcg acgtcttccc caactccgcc acccgccgct catacgtcat ttcgcaccaa
     1981 ccgtgcttcc cgcgatagcg aaacacaatc ttgtgcccgg gctcaatctt tgcccgcgaa
     2041 cgtaccgcgg aatccgatag ctccacccac tgcccgttca ccagatagct gccttcccac
     2101 ccgcgcaact cgaatttccc gtcgttcttc agcgtaagcg gcgccgccgt gcagggcacc
     2161 accttccccc gcaccttggc ctccgcgcat tgataccgcc cagaaacatc cagcacctgt
     2221 gccctgctct gactgggcag cacgaggcca agcaacgaca atacaatcac ataggagacc
     2281 ctcaccgccg accttggtct catttcccag cctccggaca caggagcgtt gctgcggacg
     2341 cccacctgtc ctcaggtaca ggctgctgca cgtgacttgc caacggcagc cgcctcatct
     2401 ctatgacttc attgtactta ttctattctg cccgtccgta cagccgctct aatcgtacca
     2461 ttcgttccct ttgtcgcgct cccgacggta ctctattaca aaatccgcga cgctctccta
     2521 ccatgctccc gctcctcctc caccggccaa gccgtctcag ttcggcacct ctgccgacgc
     2581 ctgcccatcc ttcctcggat ccgacgcccc atagttcacg cccgtcgcaa agtcccgcag
     2641 caccgcttgc ccgcctccca ctcccgacga atacatcccc agcacctgaa tcttgtgccc
     2701 tttccccgac aattcattcc gcgccgccgc tggaatccgg ttctccatca gcacatcaca
     2761 cccgtcaaac gaatgctttg aaaatctcgg agcctccatg gccgcctgaa tgttcatctt
     2821 gaaatccgca acgttcgcga tgaattgcgc atgcgcctgc gactggttcc atccgcccat
     2881 gatgccgaac ccgatgtgca tgtcaccctt ctgcatgaag gccggaataa tcgtgtgcag
     2941 cgggcgtttg tggccagcca gcgagttcgg caaacccgga gtcatgccaa agc
//
LOCUS       DASTPM010000507         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_566170, whole genome shotgun sequence.
ACCESSION   DASTPM010000507 DASTPM010000000
VERSION     DASTPM010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566170"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFR42_09000"
     CDS             <1..1243
                     /locus_tag="VFR42_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0369334.1"
                     /translation="ECTITRLIKDGDRIAGAFGYWRETGRFVVFKAKAIVIATGGIGK
                     AWKITSNSWEYTADGMALAYEAGADLLDMEFVQFHPTGMVWPPGVQGLLVTEAVRGEG
                     GILRNKNGERFMEKHDPKRMELSTRDVVARAIYSEVREGRGTPHGGAFLDISHKPAEY
                     VKKKLPSMYHQFKELADVDITTGPMEVGPTCHYMMGGIRVDAETAQSTIPGLYAAGEA
                     AAGLHGANRLGGNSLSDLLVFGRRAGKAAAEYAKSAAGSALDSAQLEAAEREMLAPFE
                     HNGEYPYEIHRDLQETMQSLVGIFRNEEDLRNALEHLEQLKPRVNQVRIDGSRMFNPG
                     WHLTRDLKSMLLISEAVTRSALARQESRGAHSRIDYPKLDDAWGKMNNIISEQGGTMA
                     LRQEAVREMPADLQKLLAETK"
     gene            1273..2034
                     /locus_tag="VFR42_09005"
     CDS             1273..2034
                     /locus_tag="VFR42_09005"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU0369335.1"
                     /translation="MSASSDDVMMMDEAVLRVHRGGPEGGAPVDYRVPIAPGMVVLDA
                     LHSIQAIQAPDLAVRWNCKAGKCGSCSAEVNGRPRLTCKTRMDSLPLNEPITVAPLKS
                     FPLIKDLVTDVSWNYKVNRKIPAFQPKAGVEWKMDQRDVDRVQEFRKCIECFLCQNVC
                     HVLRDHDKHDSFAGPRFFVRVAGLEMHPLDGVSRTKLLKDEMGLGYCNITKCCTEVCP
                     EEIHITDNAIIPLKERVVDEFYDPVMMLLRKIRGA"
     gene            2060..2566
                     /locus_tag="VFR42_09010"
     CDS             2060..2566
                     /locus_tag="VFR42_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369336.1"
                     /translation="MDYQLKAISKSGIAEALAKVDLYRYLNEPEESESICRDILAVDA
                     NQQMALRMLGLAMTDQFTGSIGDRYREVEEIFGRLADRYEQMYYTGLYYERRAKAQLR
                     TGQSPHVVYPLFERALHCFGEAEKIRPAGNDDSILRWNRCVRLLQTPSMVWDEQLATY
                     DAEDAPPR"
     gene            complement(2602..2796)
                     /locus_tag="VFR42_09015"
     CDS             complement(2602..2796)
                     /locus_tag="VFR42_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU0369337.1"
                     /translation="MIAVLEKIGFTLSRQSGGHRIYKNAAAGKRVAVPFHAAKVLHPK
                     VIKSILLDADLSVDRLNEFL"
BASE COUNT          620 a          837 c          858 g          582 t
ORIGIN      
        1 ggaatgcacc atcacgcgcc tgatcaagga tggcgatcgc atcgccggtg cgttcggcta
       61 ctggcgcgag accggccgct ttgtggtgtt caaggcgaaa gccatcgtca tcgctaccgg
      121 cgggatcggc aaagcctgga aaatcacttc caattcgtgg gagtacaccg ccgatggcat
      181 ggccctcgct tacgaagccg gcgcggacct gctggacatg gaatttgtgc agttccatcc
      241 caccggaatg gtctggcctc ccggtgtgca aggcttgctg gtcactgaag cggtgcgcgg
      301 cgaaggcggc atcctgcgca acaaaaatgg cgaacgcttc atggagaagc acgatccgaa
      361 acgcatggag ctttccacgc gcgatgtcgt cgcgcgcgcc atttatagcg aagtgcgcga
      421 aggccgtggc acgccgcacg gcggcgcgtt cctcgacatt tcgcacaagc cagcggagta
      481 tgtgaagaaa aaacttccca gtatgtatca ccagttcaag gaactcgccg acgtggacat
      541 cacaaccggg ccgatggaag tcggcccgac ttgccactac atgatgggcg gcattcgtgt
      601 ggacgcggaa accgcgcagt ccacgattcc cggtttgtac gcagcgggag aggcggcggc
      661 cggtctgcac ggcgctaatc gcctcggcgg caattcgctg tcagatttgc tggtgttcgg
      721 ccggcgcgct ggaaaagccg ctgccgaata tgccaagtcc gcagcgggct cggcgctcga
      781 ttccgcgcaa cttgaagcgg ccgagcgcga aatgctggcg cccttcgaac acaacggtga
      841 atatccctac gaaatccatc gcgacctgca ggaaaccatg caatcgttgg tcggcatctt
      901 tcgcaacgag gaagatctgc gcaacgcgct cgaacatctc gagcagctga agcctcgcgt
      961 gaatcaggtg cgcattgacg ggtcgcgcat gtttaatccc ggctggcacc tcacccgcga
     1021 cctgaaatcc atgctgctga tttccgaagc ggtgactcgc agcgctttag cgcgtcagga
     1081 aagtcgcggc gcgcatagcc gcatcgatta ccccaagctc gatgacgctt ggggcaagat
     1141 gaacaacatt atttccgagc aaggcggtac gatggccttg cggcaagagg cggtgcgcga
     1201 aatgccggcg gatttgcaga aacttttggc ggagacgaag tagtcgagac acatctgacc
     1261 gagcgaggcc gaatgagtgc atcgagcgac gacgtgatga tgatggatga agcggttctg
     1321 cgcgtgcacc gcggcgggcc cgaaggcggc gcgccggtgg actatcgcgt tccgatcgcg
     1381 ccgggtatgg tcgttctcga tgcgctgcat tccattcagg caatccaggc ccctgatctc
     1441 gcagtgcgct ggaattgcaa agcagggaag tgcggttcgt gttccgcgga agtgaacggc
     1501 cgcccgcgtt tgacatgcaa aacccgcatg gattccctgc cgctgaacga gccgatcact
     1561 gttgcgccgc tgaaatcctt tccgctcatc aaagacttgg tcaccgacgt ttcctggaat
     1621 tacaaggtca acagaaagat tccggcgttc cagcccaaag cgggggtcga atggaaaatg
     1681 gaccagcgcg atgtcgatcg cgtgcaggaa ttccgcaagt gtattgagtg ctttctgtgc
     1741 cagaacgttt gccacgtgct gcgcgatcat gacaagcatg acagctttgc cgggccgcgc
     1801 ttctttgtgc gcgtcgcggg gctcgaaatg cacccgctgg atggagtttc gcgcaccaaa
     1861 ctcctgaaag acgaaatggg attgggctat tgcaatatca ccaaatgctg caccgaagtt
     1921 tgtccggaag agatccacat caccgataac gcgatcattc cgctgaaaga gcgcgtggtg
     1981 gatgagtttt acgacccggt gatgatgctg ctgcgcaaga ttcgcggcgc ctgattctta
     2041 acgaccgcgg gaggcttgta tggactacca actcaaggcc atctccaaat ccggaatcgc
     2101 cgaagccctc gccaaagtcg atctgtaccg ttatctcaac gaaccggagg aatcggaatc
     2161 catctgccgt gacatcctgg ccgtggatgc gaatcagcag atggcgctgc gaatgctcgg
     2221 tctggccatg accgatcagt tcaccggcag catcggcgac cgttaccgcg aagtggaaga
     2281 gattttcggg cgcctggcgg atcgctacga gcagatgtat tacaccggcc tttattacga
     2341 gcgtcgcgcc aaagctcagt tgcgcaccgg ccagtcgcca cacgtggttt atccactgtt
     2401 tgagcgcgcg ctacactgtt tcggtgaggc ggaaaaaatc cgccctgccg gcaacgatga
     2461 ctccatcctc cgctggaacc gctgcgtccg cctcctgcaa acgccgtcga tggtctggga
     2521 cgaacagcta gctacctacg acgccgaaga cgccccaccc cgttaaattt ccgaatagtt
     2581 gcgctggtgt aggaatctga tttacagaaa ttcgttcaga cgatcgacgc ttaagtcagc
     2641 gtccagcagg atgctcttta tcaccttggg gtggagcacc tttgccgcat gaaacggtac
     2701 agcgacccgc ttaccggcgg cggcattttt gtaaatccta tggccgccgc tctggcgcga
     2761 caaagtgaat ccgatctttt ccaaaacggc gatgatttcg ctgtagaccc ttcgctgcgc
     2821 cgaaggtcaa cgcaattccc ttgcttgtgt gtcacctcgt ttgaccgttg ctaccgcggg
     2881 tgctaggctg cccgttc
//
LOCUS       DASTPM010000508         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_392967, whole genome shotgun sequence.
ACCESSION   DASTPM010000508 DASTPM010000000
VERSION     DASTPM010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392967"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /gene="dnaX"
                     /locus_tag="VFR42_09020"
     CDS             complement(<1..1579)
                     /gene="dnaX"
                     /locus_tag="VFR42_09020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU0369338.1"
                     /translation="MSYQVIARKWRPQTFKDLVGQEHVTGTLANAIKNDRVAHAYIFS
                     GARGVGKTTAARILAKALNCVNGPTSEPCGVCDSCKEISAGSSLDVIEIDAASNRGID
                     QIRELREMVRYAPAASRSKVVILDEAHMLTGEASNALLKTLEEPPDRVIFVMATTEPE
                     NLEDTIRSRSQHFHFRALTFNEIAERIKYIAKEEGLSIADGAVAVIARMAEGSMRDAL
                     SLLEQARAYCGDNIPDRDVRELLGVVPEDALSELVEAIAAGSADRALGLVHTFQKEGR
                     NLQHFCREAIRHMRNLLITRVCGADSDLIAATQDQRPALAKAAAQFSEEDLTRFFNVL
                     LQTDDDLRRKPDPRVHLEMGLLRLIHAGRLASLEELLSELGGENKIPAAKGAAGQRAS
                     AQVASPAWSPKNEIANAIAAAAAPSFTSRPADASTPATPAAFSTLAWRTCEPPKTSAP
                     AAPTFAPASAPAIVAPAKIAEIDANAAQEIQEPETTALSAQNAKSTPQVPPAGHTSED
                     AATNGSANSVSGKGISDD"
     gene            complement(1764..1862)
                     /gene="ffs"
                     /locus_tag="VFR42_09025"
     ncRNA           complement(1764..1862)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR42_09025"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1971..2468)
                     /locus_tag="VFR42_09030"
     CDS             complement(1971..2468)
                     /locus_tag="VFR42_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0369339.1"
                     /translation="MKISLKGTLLVAAVTAIVGVAAFAQEKKEAAGGHGIVHTADLKW
                     TPIIKGCEVASVSGDMNADGIPFVMRLRCAAGTKIPAHWHPTEENVTVLKGVFQVGMG
                     EKFDATGLQTMNLGDFGSIPKEMRHFALSKTATVVQVNGTGPFKVNWVNPADVVPPDA
                     AKKKM"
     gene            2815..3519
                     /locus_tag="VFR42_09035"
     CDS             2815..3519
                     /locus_tag="VFR42_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369340.1"
                     /translation="MKPFVRALFVIASFSLAASFAHAEPRQLIQGTQIHLKLTSDIGT
                     ASSRNGDPFIAVTTEPLMMGDQVILPAGTRIRGIVTSINRARRFSLFRGEAYMNLTFR
                     SIEVDSRLIPVQMSVLAVQKPSEEGEGRQRKDIDVTEGQLLQQKHDVKGDIIAGTIGT
                     GGSTLIGKLASHAAAGFGIGLAGSAIYVSARKGKELSLPAETGMLVRMDSTVTVPTFN
                     GNVGSSANEGTTSGSK"
     gene            complement(3609..>3908)
                     /locus_tag="VFR42_09040"
     CDS             complement(3609..>3908)
                     /locus_tag="VFR42_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0369341.1"
                     /translation="AGILAMVPDRSNKVQAPEPLKPTNRFGSPQAAIKHFVESRATTE
                     DFLKKTADLRGHAVDSPGGGGKWDAYEFILLIGAHSERHTKQIEEVKTSPGYPAK"
BASE COUNT          733 a         1003 c         1326 g          846 t
ORIGIN      
        1 ggtcgtcgga aattcctttg ccggaaacgg aattggcgga accgttggtg gcagcgtctt
       61 ctgacgtgtg tcctgctggc ggaacttgcg gggtggactt ggcattctgt gcggacaggg
      121 cggtggtctc gggttcttgg atttcctgcg cggcattagc gtctatttct gcgatttttg
      181 cgggcgcgac gattgcgggg gcgctcgccg gagcgaaggt tggagccgct ggcgcggaag
      241 tttttggggg ttcgcaggtt cgccacgcta gcgtggagaa tgcagccgga gttgccggtg
      301 tgctggcgtc tgctggtcgg gatgtaaacg acggcgctgc ggcggcggcg atggcgttgg
      361 cgatttcgtt tttgggcgac caggcaggag aagcgacttg cgcggatgcg cgctgaccgg
      421 cggcgccttt cgcagcggga atcttgtttt cgccgccgag ttcggagagc aattcttcga
      481 gagaagcgag gcggccggcg tgaatcaggc ggagcaagcc catttcgaga tggacgcgtg
      541 gatcgggctt gcgacgcagg tcgtcatcag tctgcaagag cacgttgaag aagcgagtga
      601 gatcttcttc actgaattgc gcagcggcct tggcgagggc tgggcgctgg tcttgtgttg
      661 cggcgatgag atcggaatcg gctccgcaga cgcgcgtgat cagtagattg cgcatgtggc
      721 gaatggcttc acggcaaaaa tgctggaggt tgcggccttc tttctggaac gtgtggacga
      781 ggccgagagc gcggtcggcg gaaccggcgg cgatggcttc gacaagttca ctgagcgcgt
      841 cttcaggaac tacgccgagg agttcgcgaa cgtcccggtc gggaatgttg tcgccgcaat
      901 aggcgcgagc ctgttctagg agggagagcg catcacgcat gctcccttcg gccatgcggg
      961 cgatgacggc aacggcgccg tcggcgatgc tcaggccttc ttccttggcg atgtacttga
     1021 tgcgctcggc gatttcattg aaggtgagcg cgcgaaaatg aaaatgctgc gagcgggagc
     1081 ggatggtgtc ttcgagattt tccggctcgg tggtggccat gacaaagatg acgcggtcgg
     1141 gcggctcttc gagagttttg agcaatgcgt tggaagcttc gccggtgagc atgtgggctt
     1201 cgtcgaggat gacgactttg ctgcgcgacg cggcgggagc gtagcgaacc atttcgcgga
     1261 gttcgcggat ctggtcaatg ccgcgattgg aggcggcgtc gatttcgatg acgtcgagag
     1321 aagagccggc ggaaatttcc ttgcaggaat cgcagacgcc gcagggttcg gaggttgggc
     1381 cgttcacgca attcaaggcc ttggccagga tgcgggcggc agtggttttg ccgacgccgc
     1441 gcgcgccgga aaaaatgtag gcgtgagcaa cgcgatcatt tttgatggcg ttggccaaag
     1501 tgccggtgac gtgttcctgt ccgacgagat ccttgaaagt ttgcgggcgc cattttctcg
     1561 caatgacttg atagctcatg ggacgaagtt tcgcacgatg gagaagggaa gtcgacggtc
     1621 gacagtcaaa agtcgaagga aaacagaacg ggaaagatct gggtgataca aaacgagtgc
     1681 acttggatcg gcggattatg ctgaatgtcg ggaaaagact tctgcattgt gaactgtgga
     1741 ccgtcgagtc gattcggtgc gaggaacttc gggtgatctg cggcacccga ggtgttcccg
     1801 gtaccgttgc tcccttccgg gcctggcggg gttcgcggga cctcgttgca cagagcccga
     1861 agctccacgc ttcggcagtc gcggacttca cgactgccca gggtcagttt aacacatcaa
     1921 acgcagcgct ttgcgaaacg gctaagtgaa gaattttgtt tttgcgcggc ctacattttc
     1981 tttttcgctg cgtcgggcgg caccacgtcg gcgggattga cccagttcac cttgaacggg
     2041 cccgtgccgt tcacctgcac gacggtcgcg gttttgctga gcgcaaagtg gcgcatctct
     2101 ttcgggatgc tgccgaaatc gcccaggttc atggtttgca gtccggttgc gtcgaatttc
     2161 tcgcccatac cgacctgaaa gacacccttt aatacggtga cgttttcttc ggtaggatgc
     2221 cagtgcgccg gaatctttgt tccagcggcg cagcgcagtc tcattacaaa cggtatacca
     2281 tcggcattca tgtcgccgct caccgatgcg acttcgcatc ctttgatgat cggcgtccac
     2341 tttaggtcgg cggtgtggac gatgccgtgt ccacccgcgg cttctttttt ctcttgagcg
     2401 aaagcggcca cgcccacgat cgcggtaact gcggccacca acagagttcc cttgagagaa
     2461 attttcattg gcaatccctc cgacacgagc aagattggct gcatgaaaaa caatgttggg
     2521 cgaaacatac gccgcctgag gatttggcgc aaggccggtt ttggttcagc gtggcccggg
     2581 gggccgcgtc aaactgcata gaattgtgtt ttcttgccac gaaatgctgt taggctattg
     2641 gccggatgga gatcctcccc atcctcccgg caagtgcccg cagcgcttga cgggtccgcg
     2701 cgccagcgtc gcacgacgag gcgtccctcc gcgtgcatcc acgtcacgga accagcctgc
     2761 tattacaagc agaagcttgg ggcgggcaac ccgcatccgc gggaggaggc atacatgaaa
     2821 cctttcgtaa gggctctgtt tgtgattgcg tcgttttcgt tggccgcgtc gtttgcgcat
     2881 gcggaacccc gccagttgat ccaaggcacg caaatccatt tgaagttgac gagtgatatt
     2941 ggcacggcat cttcgcgcaa tggcgatccg ttcattgcgg tgacgacgga accgttgatg
     3001 atgggcgatc aggtgattct gccggcagga acgcgcatcc gcggaattgt gacgagcatc
     3061 aaccgagcgc ggcggttttc gctgttccgc ggcgaggcct acatgaactt gacctttcgc
     3121 tcgattgaag tggacagccg gctgattccg gtgcagatga gcgtgctggc ggtgcagaag
     3181 ccgtcggaag agggggaagg ccggcagcgc aaggacatcg atgtcaccga gggacagctg
     3241 ctgcagcaga agcatgacgt gaagggcgac atcattgccg ggaccatcgg gacgggcggt
     3301 tcaacgttga tcgggaagct ggcgagccat gcggcagccg gattcgggat tgggctcgcg
     3361 ggcagcgcga tttatgtttc ggcgaggaag ggcaaggaac tgagtttgcc ggcggagacg
     3421 ggaatgctcg tgcggatgga cagcacggtg accgtgccga cgttcaacgg caacgtgggc
     3481 agcagcgcga acgaggggac gacatcgggc agcaagtaag acggaaggcg atttagcgag
     3541 acgcgaaccg cgcgaagagc gttattcgat tggcgcggtt cgatgcttct cgccggcctg
     3601 caaagagttt acttcgccgg gtagccgggg ctggtcttta cttcttcgat ttgcttggta
     3661 tggcgctcgc tatgcgcacc aatcaggaga ataaattcgt aggcgtccca cttgccaccg
     3721 ccgcccgggc tatccacggc atggccgcgc aggtcggcgg tcttcttgag aaagtcttcg
     3781 gtggtggcgc ggctctccac gaaatgtttg atggctgctt gcggtgaacc gaagcggttg
     3841 gttggcttga gaggttcggg cgcctgcacc ttgttggagc gatcgggcac cattgctagg
     3901 attcccgc
//
LOCUS       DASTPM010000509         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1258106, whole genome shotgun sequence.
ACCESSION   DASTPM010000509 DASTPM010000000
VERSION     DASTPM010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258106"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VFR42_09045"
     CDS             <1..1015
                     /locus_tag="VFR42_09045"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0369342.1"
                     /translation="DDNEMPLRALQSGAANVLGGTSWLWARPEYFEAVDVLFIDEAGQ
                     MALADVIAVAQAAKNLVLIGDPQQLERPLKGSHPPGAEKSALQHLLGDRKTIPDTAGL
                     LLPETWRMHTAICNFTSEMFYDGRLGTRGLTKFRVLQGHPWLSGAGLWFVPVVHEGNR
                     NSSPEEIEVIATIVESLLEPGVTWTRSTGNTKPIELKDILIVAPYNAQVAELLARLPE
                     GACVGTVDKFQGQEAAVVIYSLTTSSPEDAPRGMEFLYSLNRLNVATSRAMSNVIVVG
                     SPRLFEPECRNPRQIQLANALCRYLEMATTVDLRGTPNTHKAEPADQPSSAPAGTGQL
                     SFF"
     gene            complement(1147..1830)
                     /locus_tag="VFR42_09050"
     CDS             complement(1147..1830)
                     /locus_tag="VFR42_09050"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU0369343.1"
                     /translation="MNSRMLALTRLGSLRTILQIAALAAVCALGANSLPAQAAAPGSA
                     SAGKVAVINVRQAIASTSEGKQAGAELQSQFAPRQSELEGLNKQINDLRQRIDAGSGK
                     ISPEEQSRLQREGEAKARALQRKQDEYQEDVNAAQADVFDRIGRKMIDVLDRYARENG
                     YVVVLDSSAQNTPILFASTNVDVTQDIVKLYDQAYPVKSGAAPASTTPARPAQKPAAQ
                     PPAAKPPQR"
BASE COUNT          418 a          615 c          558 g          440 t
ORIGIN      
        1 ggacgacaat gaaatgccct tgcgcgcctt gcaatccggc gcggctaacg tgctgggcgg
       61 aacgtcctgg ctgtgggcca ggccggaata ttttgaagct gtggacgtcc tgttcatcga
      121 tgaggccggg caaatggctc tggccgacgt cattgccgta gcgcaagccg ccaaaaacct
      181 cgttcttatc ggggatccgc agcaactgga acgcccgctc aagggcagcc atccgccggg
      241 agcggagaaa tccgcgctgc aacatctgct cggggaccgc aagaccattc ccgatacggc
      301 gggcctactg cttcccgaaa cgtggagaat gcacacggcg atttgtaact tcacttcgga
      361 aatgttttat gacggccggt tgggaactcg aggccttacc aagtttcgtg ttctgcaagg
      421 acatccgtgg ctaagtggag caggcttgtg gtttgtgccg gtggttcatg aaggcaatcg
      481 caattcttct ccggaagaga ttgaagtcat tgcgacgatt gtggaaagcc tgttggaacc
      541 gggcgtgacc tggactcgga gtaccggcaa caccaagccc atcgaactca aggatatttt
      601 gatcgtcgcg ccctacaacg cacaggttgc cgaactgttg gcgcgactgc ccgagggcgc
      661 ttgcgtcggc acggtcgaca aatttcaagg gcaggaagct gcggtggtca tctattcgct
      721 gaccacttct tcgcccgaag atgccccgcg cggcatggaa tttctctata gcctcaaccg
      781 gctgaacgtc gcgacttcgc gcgctatgtc caacgtcatc gttgtaggca gccctcgcct
      841 cttcgagccg gaatgccgca atcctcggca aatccagctt gccaatgctt tgtgccgcta
      901 cctggaaatg gccaccaccg tagatcttcg cggcacacca aatacacata aggcggagcc
      961 agcggaccaa ccgagctccg ccccggctgg aaccggccag ttatcgtttt tctagagggt
     1021 cgaggaagat tgattccgtc aacgcgaaaa gaaaactaag ggaagcaacc ccgagttcag
     1081 ctcaaaaaat aaaacgcccc ggaatccagc tccggggcgt ccttgaatta tgagaataat
     1141 cgcgagttat cgctgcggtg gtttcgctgc aggaggctgc gcggccggct tttgcgcagg
     1201 ccgagcggga gtcgtgctgg ccggcgccgc accgctcttc accggatacg cctgatcgta
     1261 aagcttcacg atgtcctggg tgacgtccac gttcgtcgac gcaaacaata tcggcgtgtt
     1321 ctgcgccgag ctgtccagca ccaccacgta tccgttttcg cgcgcatagc ggtccagcac
     1381 gtcgatcatc ttgcgcccga tgcgatcgaa cacgtcggct tgcgcggcat ttacgtcttc
     1441 ctgatattca tcctgcttgc gctgcagggc ccgcgctttg gcttcgcctt cgcgctgcaa
     1501 ccgcgactgc tcttccgggc tgatcttgcc gctcccggcg tcgatgcgct ggcgcaagtc
     1561 gttgatctgc ttgttcaatc cttccagctc actctgccgc ggcgcaaatt gcgattgcag
     1621 ttccgcaccg gcttgcttgc cttcggaggt gctggcgatc gcctgccgca cgttgatcac
     1681 cgcgactttc cccgccgaag ccgagcccgg cgcggcagcc tgtgcgggca gcgaatttgc
     1741 accgagagcg caaaccgcgg cgagcgccgc aatctgaagt attgttctga gcgaccccaa
     1801 tcgggtcagg gccaacattc tggaattcac gtttttctcc ttctttcttt cgaattatct
     1861 gaaaatcgaa ttttcaaaat ccctacgacc aaaaaccgac atccgccaat atctaagtca
     1921 aatacttaaa tccggaatcc ttttatcctg cctacggtgc gctttcctgg ccctcacaca
     1981 aattttgcag tatagctgac gcccgaactc cccgcaaccg aaagggccaa g
//
LOCUS       DASTPM010000510         3893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1286717, whole genome shotgun sequence.
ACCESSION   DASTPM010000510 DASTPM010000000
VERSION     DASTPM010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286717"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1964)
                     /locus_tag="VFR42_09055"
     CDS             complement(186..1964)
                     /locus_tag="VFR42_09055"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEU0369344.1"
                     /translation="MFLRKLRVHKDGKEHGYWSLVETVRTADGPRQRTLCYLGELNGS
                     AHARWQKTVEVFNEHGESTQLKLFPSEAEVPDDPNVARVLLKKVRVERTRRFGDCYLG
                     LELWKRLGLDEFFAQHLDGDDADVAWSRVAAVLAINRLCAPGSELAVEQHWYPSTALD
                     DLLHIVKGKINDTRLYRCLDRLLPLKTKIEQHLKQRFGELFQAEFDVLLYDLTSTYVE
                     GAAEENPMIRRGYSRDHRPDCEQLVLALIVNQDGFPFSYELFDGNRADVSTVEAILRT
                     VERKHGKARRVWIFDRGVVSEENLVAIRKRGGQYLVGTPRSKLKQFEQELLKDDFEKI
                     RPEVEVKQMRIPGGEETYILCRTAGRKEKEKAIRSRFVAKIEKALTGLEKRIADGKLK
                     DRGKMFMGLGRIQASHPQVADLYEMALKDTQQGARLVWRQKPEQQQWLEAREGAYLLR
                     TNLTADGAADLWKKYMQLTEVEAAFRTLKSELAVGPLFHQLERRVKAHVLVAFLGYAL
                     LVTLKHLLKRGGAEYSPANVLKRLAEVYSVDIVLPTLEGREIWLRRISKLDDQQQKIL
                     FQLQLPLPERLEPTQILKCSENSAIA"
     gene            complement(2231..3712)
                     /locus_tag="VFR42_09060"
     CDS             complement(2231..3712)
                     /locus_tag="VFR42_09060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0369345.1"
                     /translation="MAATILGSSMAFIDSTVVNVALPTLQTTFRASVVDVQWVVESYG
                     IFLSALILAGGALGDLFGRRRIFVIGVGVFAAASIACGLSSSIQQLITARSAQGIGAA
                     LLVPGSLAIISAGFDEESRGKAIGTWSGFTAITAALGPVLGGSLIQYISWRWAFLLNV
                     PLAVAVIAISLRHVPESRRAETKRMDWLGALLATVGLAGVVTGFLESGRLGWRNPLVF
                     GGLLGGFVLLAAFVALEVRVPSPMVPLTLFRSRSFLGANLFTLLLYGTLGIFLFLFPM
                     ALIQLQEYSATAAGSAMLPMILLMFLLSRWSGGLVKRYGGRIPLVVGPLVVAAGFVLF
                     AVLLARGSYWNTYFPAALLLGLGMAITVAPLTTVVMNSVAQRHAGTTSGINNAVARLA
                     GVLAIALFGIVMVKVFDGRLDHHLAKLNLPENIVQDLRSREIELAGLQPLPGLDTDTV
                     AAIRRAISESFISGFRIVLFCCAGLSITSALIAWKLLDPVLRE"
BASE COUNT          896 a         1173 c         1030 g          794 t
ORIGIN      
        1 ggctaggccg gcgcctgctg ccgcgccgac gactaaggat tgcccgcagt gcgcgatgcc
       61 gattccgctg gcggcgagaa agtgtgggca ctgcacgtcg gcagtctaaa acaagcagtt
      121 cctcagggct aacccatgtt cgtcactaag agggctgaaa gagggttaag tcctttgaaa
      181 tcagtttagg ctatggccga gttttcacta cattttagga tctgagttgg ttcgagtcgc
      241 tccggtaacg gcagttggag ttggaaaaga attttttgtt gttggtcatc gagtttgctg
      301 atgcgccgca accagatttc tcggccttcg agtgtgggca gcacgatgtc cacgctgtat
      361 acctctgcga gccgcttcag tacgttcgcc ggcgaatact ctgcgccgcc tcgtttcagc
      421 agatgcttca gtgtcaccag cagggcataa ccgagaaagg ccaccagcac gtgcgccttg
      481 acgcgtcgtt ctagttgatg gaagagcggg ccaacggcca gttcgctctt cagtgtgcga
      541 aacgcggctt ccacttcggt cagctgcata tactttttcc aaagatctgc agcaccgtcg
      601 gctgtcagat tggtgcgtag cagataggct ccctctctgg cctccagcca ttgctgctgc
      661 tcgggcttct gccgccagac caggcgtgca ccttgttggg tatccttcag cgccatttca
      721 taaagatctg ctacctgtgg atgcgaagcc tgaatgcggc ccaaccccat aaacatcttg
      781 ccgcgatcct tcaatttgcc gtctgcgatg cgcttctcta gtcccgtgag cgctttttcg
      841 atcttcgcca caaagcggct gcggatagct ttctccttct ccttccgccc cgccgtgcgg
      901 cacaggatgt aggtctcttc gccacccgga atccgcatct gcttcacctc tacttccgga
      961 cggatctttt cgaaatcatc cttgagcagt tcctgttcaa actgcttgag cttgctgcgt
     1021 ggtgtgccca ccagatactg tccgccgcgc ttgcggatgg ccaccaggtt ctcctcactc
     1081 accacgccgc gatcgaaaat ccatacccga cgtgctttcc catgcttgcg ctcaaccgtg
     1141 cgcaggatcg cttcgacggt ggaaacatcg gcacggttcc catcgaaaag ctcatagctg
     1201 aagggaaacc cgtcctggtt aacgatcagt gccagcacca gttgttcgca gtccggacga
     1261 tggtcgcgcg agtagccccg ccgtatcatg gggttctctt ccgcggctcc ctccacatac
     1321 gtgctggtca ggtcatagag cagcacatcg aactcggcct gaaataactc gccgaagcgc
     1381 tgtttcagat gctgttcgat cttggtcttc agggggagca agcgatccag acaacgatag
     1441 agtcgcgtgt cgttaatctt gcctttcacg atgtgcaaca gatcgtccag cgccgtggac
     1501 ggataccagt gttgctccac agccaactcg ctgcccggcg cgcacagccg gttgatcgct
     1561 aacaccgcgg ctacccgcga ccaagccaca tcagcatcat ccccgtccag atgctgcgca
     1621 aagaactcat cgagccccaa acgcttccat aactccagtc ccagatagca gtcgccaaag
     1681 cggcgcgtgc gttcgacgcg gacttttttc agcagcactc gcgctacgtt cggatcatcg
     1741 ggtacttctg cttcggaagg aaacagcttc aactgggtgc tctcgccgtg ctcgttgaaa
     1801 acctccacgg tcttctgcca acgcgcgtga gccgaaccgt tcagctcccc gagataacaa
     1861 agcgtgcgct gccgcggacc atcggcggta cgcaccgtct ccaccagcga ccagtagcca
     1921 tgctccttgc cgtccttgtg gacgcggagc tttcgcagaa acatgcaggc aatctgtcac
     1981 ggttgcatca ctcaagcaag gaggtaggtt ttcactacaa ccactttccg aacacaccgt
     2041 gttggcaggc aaagacttcc cgacgaacta ctcacgaaaa aattcaaaaa gtgatgttca
     2101 atgacgaact tcggctaaga acttaaccga cgtgctcgat ttgctccggc tgtataagag
     2161 tgtggcaaaa cccttgcccc aagttattgg aattgcattt tttgcgacga cttggaatcg
     2221 gcggtcgaca ctattctcta aggacaggat caagaagctt ccaggcaatt agagcgctgg
     2281 tgatcgataa tcctgcgcaa caaaaaagaa cgatacgaaa tcccgaaatg aaactttcgg
     2341 agatggcgcg ccggatcgcc gcaacggtat ccgtatccaa gcctgggagt ggctggagac
     2401 cggcaagctc gatttcccgt gaccgaagat cctgaacgat gttctcgggt aggttcagct
     2461 tcgcgagatg atgatccagg cgcccatcaa aaaccttgac catcacaatt ccaaacaggg
     2521 cgatagccaa aactccagct agtcgggcca cggcattatt gatacccgac gtggtccctg
     2581 catgtcgctg agcgaccgag ttcatcacta ccgtagtgag cggggccaca gtgatcgcca
     2641 ttcccaaacc aagcagcaac gcagcaggaa agtaggtgtt ccagtagctg cctcttgcga
     2701 gtaacaccgc aaacaggacg aaacccgccg cgacaaccaa cggccccaca accagcggaa
     2761 tccttccgcc gtaacgctta accagccctc cggaccatcg cgacaaaagg aacatcagca
     2821 agatcattgg cagcattgcg gagcccgctg ccgtcgccga atactcttgc agctggatca
     2881 gggccatcgg aaatagaaaa aggaaaattc cgagtgtgcc gtaaagaagc agtgtgaaga
     2941 gattcgcgcc cagaaacgag cgagacctga atagcgttag cggaaccatt ggcgacggca
     3001 cccggacctc gagcgcaacg aaggcagcga gcaacacgaa accgccgagc aagcccccaa
     3061 acaccagggg attcctccag cccagcctcc cggattcgag aaatcccgtt accactcccg
     3121 ccagcccaac tgtagccagc aaggccccga gccaatccat ccgcttggtc tcggccctcc
     3181 ggctttccgg tacatgtcga agagaaattg cgataacggc aaccgccagc ggcacgttca
     3241 aaaggaaagc ccagcgccag gagatatatt ggatcagcga cccgccaagc acgggaccga
     3301 gggcggcggt aatggctgtg aagccggacc aggtaccgat ggctttgccg cgcgactctt
     3361 cgtcaaaccc ggcactgatg atcgcgaggc tgcccggaac gagtagtgcc gcgccaattc
     3421 cttgcgcaga acgtgcggtg atgagttgct gaatgctcga actgagaccg caagcgattg
     3481 aagctgccgc aaatactcct acgccgatta caaagatccg tcggcggccg aacaagtcgc
     3541 ccagtgcccc accggcaaga atcagcgctg acaggaaaat tccataggac tccaccaccc
     3601 actgcacatc gacaacgctg gcgcgaaatg ttgtctgcaa tgtaggcagg gcgacgttga
     3661 cgacggtgct gtcaatgaac gccatgctcg agccgagaat tgttgccgcc aagatccaag
     3721 gttgagctgc ctccgcactc ggatcgctgg cactggatga taggatcagg ctttcgtcgc
     3781 aaggcgttct catacaccta ccgatgcctt actctcgtct tttggtgcgg cccggacagg
     3841 ttgcattgca acacgcagag ttccccaggc gaaggagaat cgcagacatt tgg
//
LOCUS       DASTPM010000511         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1027150, whole genome shotgun sequence.
ACCESSION   DASTPM010000511 DASTPM010000000
VERSION     DASTPM010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027150"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..>2088)
                     /gene="priA"
                     /locus_tag="VFR42_09065"
     CDS             complement(173..>2088)
                     /gene="priA"
                     /locus_tag="VFR42_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU0369346.1"
                     /translation="RIAVLRAAEEDGAKSEDPSAGSGQADRRSTNRLNANQQTLIETL
                     AGAGGRVPVETLRALEIPRTTLGTLVERGLIDIVEEPAAFHVSRVKARPSPFDFQFNA
                     AQQQALRRIEEGVSAGKFGGVLLHGVTGSGKTAVYLAGMRTVLEAGRSAILLVPEIGL
                     TPAVAADLHQVFGDEVAILHSALSDQERAEQWHRIKRGEARMVVGTRSAVFAPVADLA
                     LIIVDEEQDSSYKQEETPRYHARDVAVMRAKMTDAMVVLGSATPSLESYYNAKKNKYA
                     LVELPDRVEQRPLPEVELIDMKQEFQETGQEQVISRKLAEEIRERLDRKEQVMILLNR
                     RGYSPVALCRACGSRMECRNCAVAMTHHKGSRRMVCHYCGFQTPVPKKCAECGSEYVY
                     FLGTGSEKLEELLHGMFPTARIGRLDRDTVRGNEDFERVLNALNEGQLDLLVGTQMIA
                     KGHDVHGVTLVGVVGADIALGLPDFRAAERTFQLLTQVAGRAGRGQTPGKVVLQTYFP
                     DHYAVQYAARHDFVGFYEKELQFRSWMHYPPYSALANVLIRSDQLDEALQWSGILGKW
                     FERTRHERVRVLGPAAAPILRLKRDYRYHFVLKSASREKLNMLLRTMLAYAGAQKIPR
                     TQVIVDVDALWLM"
BASE COUNT          422 a          699 c          556 g          411 t
ORIGIN      
        1 cttcctccag gcatgccact cactgacaaa agatgctgac gtcccgcaaa agcgttggtg
       61 agcaaaacgc ctctctattc aagccaaggc tgcggtcgca tattagtttc tgcccgcgat
      121 cgccctagcc aaccccaaca accaagacct aaaggcctaa catcggccgt tcttacatca
      181 gccacagcgc atccacatcc acaatgacct gcgtgcgcgg gatcttctgc gcgccggcgt
      241 aggccagcat ggtccgcagc agcatattca gcttctctcg gctggctgac ttcagaacaa
      301 agtgataccg ataatcccgc ttcaggcgca gaatcggcgc ggccgccggc cccagcaccc
      361 ggactcgttc gtgccgggtc ctctcaaacc actttcccag aatgcccgac cactgcagcg
      421 cttcgtcgag ctggtcgctg cgaatcagga cattggccag agcggagtac ggcggatagt
      481 gcatccagct gcgaaattgc agctcttttt catagaaccc gacaaagtca tggcgcgctg
      541 catactgcac cgcatagtga tcgggaaaat aagtctgcag cacgactttg ccgggcgtct
      601 gcccacgccc ggcgcgtccc gcgacctgcg tgagtaactg gaaggttctc tctgccgcgc
      661 gaaaatcagg caggcccagg gcaatatcgg cgccaaccac accaaccagc gtgacgccat
      721 ggacgtcatg tcccttggca atcatctgcg tgcccaccag gagatcgagt tggccctcgt
      781 tcagtgcgtt gagcacgcgc tcgaaatctt cattgccgcg cacggtatcg cgatccagac
      841 ggccgattcg cgcggtggga aacatgccgt gcaacagttc ttccagcttc tccgatcccg
      901 ttccgagaaa atacacatac tcgcttccgc actccgcgca cttcttcgga accggagtct
      961 gaaatccgca gtagtgacac accatgcggc gcgaaccctt gtgatgggtc atcgcgacgg
     1021 cgcaattgcg gcattccatg cggcttccgc aggcgcggca gagtgccacc ggagaatagc
     1081 cgcgccggtt cagcagaatc atcacctgct ctttccggtc caatcgttca cgaatctcct
     1141 ccgccagctt gcgcgaaatc acctgctcct gcccggtctc ctgaaactcc tgcttcatgt
     1201 cgatgagttc gacctcaggc agcggacgtt gctccacccg gtccggcaac tctaccagcg
     1261 catacttgtt cttcttcgcg ttgtagtaag actctagaga gggcgtagcc gagccgagca
     1321 ctaccatggc gtcggtcatc ttggcgcgca ttacagccac atcgcgcgca tggtagcgcg
     1381 gggtttcttc ctgcttgtaa gaggaatcct gctcttcgtc gacgatgatg agcgccagat
     1441 ctgcgaccgg cgcaaacacc gccgaacgcg tgccgaccac catgcgcgct tcgccgcgct
     1501 tgatgcgatg ccactgttcg gcgcgctcct gatcggagag cgcagaatgg agaatggcga
     1561 cttcatcgcc gaagacctgg tgtagatccg cggccaccgc cggcgtcagc ccgatctcgg
     1621 ggaccaacag gatggccgat cttccggcct caagcaccgt ccgcatccct gcgaggtaga
     1681 ctgccgtctt tcccgatcca gtcacgccat gcaacaacac tccgccaaat ttgccggcgg
     1741 aaacgccctc ttctatgcgc cgtaacgcct gctgctgtgc cgcattgaat tgaaagtcaa
     1801 agggggatgg ccgcgccttc acgcgcgaaa cgtgaaacgc cgccggctct tcgacgatat
     1861 caatcaagcc acgttcgaca agagtcccca gcgtagtgcg agggatctcc agtgcccgca
     1921 gagtctcgac cggaacgcgt ccccctgctc ccgccagtgt ctcaatcaag gtctgctgat
     1981 tcgcgttgag tctgttcgtg gagcgacggt cagcctgccc tgagcctgcc gaagggtcct
     2041 cgctcttcgc accgtcttcc tccgcagctc gcagcactgc aatcctga
//
LOCUS       DASTPM010000512         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1402170, whole genome shotgun sequence.
ACCESSION   DASTPM010000512 DASTPM010000000
VERSION     DASTPM010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402170"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1488
                     /locus_tag="VFR42_09070"
     CDS             148..1488
                     /locus_tag="VFR42_09070"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0369347.1"
                     /translation="MKRLFHVAGVALLALAAAGSAAAQSSPYPTADVQKLYQDLLGKI
                     NAIPLYDNHAHPGFADDTDVDAMAAPPDESSVLRLSDDNPEFVTAAKSLFGYPFDDFK
                     PEHAKWLAERKNAAKKSGSSAYFDQILDKLNVETCLANRAFMAPYLDTKRFHWVFFGD
                     SFFYPFDNREQTASTPDMGVYVPLQEKMLARYKKQMNVGALPADLVGYEAFVRNTMAD
                     NQKRGGVAIKYEAAYFRSLYFSDPPRERAAAIYAKYHSGGVPSADDYRIFQDYIFRVM
                     VDQAGKLKLPMHFHTAVGIGDYFSLHQGHVLNLENVLRDPRYHNTTFVLVHGGWPYEK
                     EAALLTAVKNVYLDTSFQSELLYPSQFKGVLKQLLTLFPEKMMYASDAFPFNDALGAE
                     ESFWLAAHTTRTALAAALAELVSERAITQDKALALARLYLHDNAAKLYGTGGAK"
     gene            1485..1901
                     /locus_tag="VFR42_09075"
     CDS             1485..1901
                     /locus_tag="VFR42_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0369348.1"
                     /translation="MSSTPSTGKIEAKYIKWKDVEREKLNPLIDREMITGQEIMVARV
                     FIKKGGHVPLHHHHNEQLTYILEGALKFAIDGKEIVVRAGEVLCIPSNMPHEAWGLED
                     TVDLDVFTPPREDWLNKTDDYLRRGGRQEPAPGHGG"
     gene            1908..>2345
                     /locus_tag="VFR42_09080"
     CDS             1908..>2345
                     /locus_tag="VFR42_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEU0369349.1"
                     /translation="MAERPQRNLRIAFQGEPGAFSEAAAIQLLGENIVTVPRPTFDSA
                     FHAMDEGAADALLAPVENSLAGSVVRVYDLLLDSKLTIVAETILPIEMQLIGCPGATL
                     DGLRSVASHPMALAQCEKFFLAHPGLQRVPAEDTAGSVRDVLAR"
BASE COUNT          503 a          695 c          672 g          475 t
ORIGIN      
        1 ggatgcgctc gaccgcatcc agcgtgtctc tgtaaaatgg cgtgccgttc ccgcgtaagc
       61 tgagcggtcc gcttttactc cggtactgta ggaggccgcc tttcaggcgg cctgcgatat
      121 ggcgttgtgc gacaagagga atccaagatg aaacgtttgt ttcacgtcgc gggtgtggcc
      181 ctgctggctc tggcagcggc aggatccgcc gccgcgcaat ccagccccta tcccaccgct
      241 gacgtgcaga agctctatca ggacctgctc ggcaaaatta acgccatacc gctctacgac
      301 aaccatgccc atcccggctt tgccgatgac accgatgtag acgccatggc cgctcctccg
      361 gatgagagca gcgtcttgcg cctgagcgac gacaatcccg aattcgtcac cgcggccaaa
      421 tctctgttcg gctatccctt cgacgacttc aagcccgagc acgccaagtg gctggccgaa
      481 cgcaaaaacg ctgcgaaaaa atccggcagc tcggcgtact tcgatcagat cctcgacaaa
      541 ttgaacgtgg agacgtgcct ggccaaccgc gccttcatgg cgccttatct ggacaccaag
      601 cgtttccact gggtcttttt cggcgactct ttcttttatc cgttcgacaa tcgcgagcag
      661 accgccagca ctccggatat gggcgtgtac gtgccgctgc aggaaaaaat gctggcccgc
      721 tacaaaaagc agatgaacgt gggtgcactg cccgctgacc tggttggcta tgaagcgttc
      781 gtgcgcaaca ccatggccga caatcaaaag cgcggtggcg tagccatcaa atatgaagcg
      841 gcgtatttcc ggtcgctcta tttcagcgat ccgccgcgcg aacgcgccgc ggccatctac
      901 gcgaagtatc attccggcgg agttccatcc gccgacgact accgcatctt tcaggactac
      961 attttccgtg tgatggtgga tcaggccggc aagctgaagt tgccgatgca ctttcacacc
     1021 gctgtcggca tcggcgatta tttcagccta caccagggcc acgtgctgaa tctggagaac
     1081 gttctgcgcg atccgcgcta tcacaacacg acttttgtgc tggtacatgg cggctggccg
     1141 tacgaaaaag aggcggcact gctaaccgcc gtgaaaaacg tttacctgga cacttcgttc
     1201 cagtccgaat tgctctatcc ttcgcagttc aagggcgtgc tgaaacagct gctcacgctg
     1261 tttccggaaa aaatgatgta tgcgtccgat gcatttcctt tcaacgatgc gctgggcgcg
     1321 gaggaaagtt tctggcttgc cgcacacacc acgcgcacgg cactggccgc agcgctggcg
     1381 gaactggtca gtgaacgtgc catcacgcaa gacaaggcgc tcgcgctggc ccgtttgtat
     1441 ctgcacgaca acgcggccaa gctttatggc acaggaggcg ccaagtgagc tcgacaccgt
     1501 caacaggaaa gatcgaagcg aagtacatta agtggaagga tgtggaacgc gaaaagctca
     1561 acccgctgat tgatcgcgaa atgattaccg gccaggaaat catggtcgct cgcgtgttta
     1621 ttaagaaggg cggacatgtt ccgctgcacc atcaccacaa tgagcaactc acctacattc
     1681 tggaaggcgc gttgaaattc gccatcgacg gaaaagaaat cgtggtgcgc gcgggcgaag
     1741 tgctctgcat tccttccaac atgccgcatg aggcgtgggg actcgaagac acggtggatc
     1801 ttgatgtatt tacgccgccg cgcgaagact ggctcaacaa gacggacgac tatcttcgca
     1861 gaggcgggcg tcaagagccc gcacccggcc acggcggcta gccgcttatg gccgagagac
     1921 cgcaacgcaa tttgcgcatc gcgtttcagg gcgaacccgg tgcgttcagc gaagctgccg
     1981 ctatccagtt gcttggcgaa aacattgtca ccgtgccgcg gcccacattc gattccgctt
     2041 ttcatgcgat ggacgaaggc gccgcggacg cgctactggc gccggtggag aattcgctgg
     2101 ccggttcggt agtacgcgtc tacgacttgc tgctggacag caagctcacc atcgtggcgg
     2161 aaacgatctt gccgattgaa atgcaattga tcggatgtcc cggcgcgacg ctggacggat
     2221 tgcgctcggt cgcgtcgcat cccatggcgc tggcgcagtg cgaaaaattt ttcctcgcgc
     2281 atcctggttt gcaacgcgtg cctgccgaag atacggcggg cagcgtgcgc gacgtgctgg
     2341 cacgt
//
LOCUS       DASTPM010000513         3992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_912010, whole genome shotgun sequence.
ACCESSION   DASTPM010000513 DASTPM010000000
VERSION     DASTPM010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912010"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..329
                     /locus_tag="VFR42_09085"
     CDS             84..329
                     /locus_tag="VFR42_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0369350.1"
                     /translation="MKFLSAFQPLGLLILRVALGLIFLTHGYPKLTRSAATMQSMFVQ
                     HGLPAQLVYVAGVLETFGGVLLILGLFARPAALLLAI"
     gene            330..524
                     /locus_tag="VFR42_09090"
     CDS             330..524
                     /locus_tag="VFR42_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369351.1"
                     /translation="MGVAIVKVHSGHGIMALHEYEFPLALGTSCLALATIGAGVLSAD
                     HLLFGEGSSTRPRPARPPKK"
     gene            complement(573..926)
                     /locus_tag="VFR42_09095"
     CDS             complement(573..926)
                     /locus_tag="VFR42_09095"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU0369352.1"
                     /translation="MVCNSCQRNIADGSRFCYNCGAKQVVTDAPNVAAVTGGPKKLMR
                     SSTDKKLGGVCAGLAEYLDMDTTLVRVLWLLVVLCGGTGILLYVILWLVLPLAPPPVY
                     VAAAPQAPIVPQPGN"
     gene            complement(1048..3195)
                     /locus_tag="VFR42_09100"
     CDS             complement(1048..3195)
                     /locus_tag="VFR42_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEU0369353.1"
                     /translation="MKVAKNEAPKNEKYGGLSRDTLIRLYRTMYLSRKLDDREIQLKR
                     QNKIYFQISSAGHEAVTAVMGLLLRPGVDWVYPYYRDRALCLMLGVTPLEMLLQAVGA
                     KDDPSSGGRQMPSHFGHPSFNIPSKSSCTGTQFLHAVGAAEATLYYEQHPKALAQAKK
                     APLAELAKHSADEIVYVSSGDGATSEGEFWESLNVACIKKLPLLYLVEDNGYAISVPV
                     EVQTAGGSIAKLVKSFPGLHVAEADGTDPLESYRVCQEAVNHCRERRGPALVHAKVTR
                     PYSHSLSDDEKLYKSPDEREAEAHRDPLSKFALFLVREGLLDQKQIEAIEAQVDHEVR
                     QATDQALAADPPAVESIYDNMYSPDVDPTSASFAGEPQLHGAQKTMVEMVAATLNDEM
                     SRDERITVFGEDVADASREENLKEVKGKGGVFKATAGLQRKYGSTRVFNTPLAEASIV
                     GRAIGMATRGLKPAAEIQFFDYIWPAMMQIRDELCTLRWRSNGSFKCPMVIRVAIGGY
                     LTGGAIYHSQSGESIFTHTPGLRVVMPSTALDVAGLLRTAIRCDDPVMFLEHKHLYRQ
                     PYNRSEYPGPDFTIPFGKARMVKEGTDVSIVTYGAFVHRAEVAAAQLQREGISAEILD
                     LRSLSPYDWDGICATVRKTHRVIVAYEDMLSWGYGAELAARIADELFDELDGPVKRIA
                     ATDTFCAYQPKLEDAILPQSDAVVETVKQLLEY"
     gene            complement(3401..3880)
                     /locus_tag="VFR42_09105"
     CDS             complement(3401..3880)
                     /locus_tag="VFR42_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369354.1"
                     /translation="MAHIYMTFDFGTDEEKAQQARHRLDGWKQAFRLDKKLLYKFERA
                     TPEGDGANAAEAAPARKGAAKAEKPPKAGKAGKGGSAKAKAPKEKEEKDEAAAVATGP
                     VKLHLRLAFSGHEKMTEERWLKRIPTEEPFKDAEPETVKTGDAGFAEIEGKFEGLEG"
BASE COUNT          761 a         1355 c          990 g          886 t
ORIGIN      
        1 cacccggctg ctcacgtcgc acacagcttt tgctagaatg cggagcgcgc gcgaataatt
       61 gcgttgatct catcgaggtg tccgtgaaat ttctcagtgc tttccaaccg ctcggcttgc
      121 tgattcttcg cgtcgctctg ggcctgattt ttctcacgca cggttatccc aagctcacgc
      181 gttccgccgc cacgatgcag agcatgttcg tgcagcatgg attgccggct cagttggtct
      241 acgtcgcagg cgtactcgaa acgttcggtg gtgtgctgtt gatcttggga ctgttcgctc
      301 gcccagcggc gctgcttctg gccatctaaa tgggcgtggc aatcgtcaaa gtacacagcg
      361 gccacggcat catggccctg catgaatacg aatttccgtt ggcgttagga acgtcctgct
      421 tggccctggc cacaatcggc gctggggtgc tgtctgcgga ccatctgctc tttggcgagg
      481 gcagctcgac tcgtccgcga ccggcgcgac cgccaaagaa atagctctct gccttcttcc
      541 ggattttctg gaggaacaat cagcggcggg cttcagttcc cgggctgcgg cacaatcggc
      601 gcctgcggcg cagcagcgac atagacgggc ggcggcgcca acggcaaaac cagccacaag
      661 atcacataca acagaatacc cgttccgccg cacaggacca ccagcagcca caacacgcgc
      721 accagcgtcg tgtccatatc cagatattcc gccagccctg cgcagacccc gcccagtttc
      781 ttgtccgtgc tcgaacgcat caacttcttt ggcccacccg ttaccgcggc cacattcggc
      841 gcgtccgtca ccacttgctt cgcgccgcag ttgtagcaaa accgtgatcc atccgcaatg
      901 ttccgctggc agctattgca aaccattaga gcctcccgca ggcagggctt gcccctgctc
      961 tggtaaaata tacgcctaag gaagccgaaa agttgcatta ggcagaccgc tggtcaaggg
     1021 agccacaact tgaaccgctg aaccgcttca gtactccaac aactgcttca ccgtctccac
     1081 caccgcatcc gactgcggca gaatcgcatc ttccagcttc ggctgatacg cgcaaaacgt
     1141 atccgtagcc gcaattcttt ttactggacc atccagctcg tcaaacaatt cgtccgcgat
     1201 tcgcgccgcc agctccgcac cgtagcccca gctcaacatg tcttcgtacg ccacgatcac
     1261 ccggtgcgtc ttgcgcaccg tcgcgcaaat cccatcccag tcataaggcg acagcgagcg
     1321 caaatccaaa atctccgcgg agattccttc gcgctgcagt tgcgctgcgg ccacctccgc
     1381 ccgatgaaca aaggccccgt aggtcactat gctcacgtcc gttccttcct tcaccattcg
     1441 cgctttgcca aacggtatcg tgaagtctgg ccccggatat tccgaccggt tatacggctg
     1501 ccggtacaaa tgcttgtgct ccaaaaacat caccggatcg tcgcagcgaa tcgccgttcg
     1561 caataatccc gccacgtcca gcgccgtcga cggcatcacc acgcgcagtc ccggcgtgtg
     1621 cgtaaaaatc gattcgccgc tctggctgtg atagatcgca ccgccggtca aatacccgcc
     1681 aatcgccacg cggatcacca tcgggcactt gaagctgccg ttcgatctcc agcgcagcgt
     1741 gcacagctca tcgcgaatct gcatcatcgc cggccagatg taatcgaaaa attgaatctc
     1801 cgccgccggc ttcaacccac gcgtggccat tccgattgcc cggcccacaa tcgacgcttc
     1861 cgccagcggc gtgttgaaca cccgcgtcga accgtacttg cgctgcaatc ctgccgtcgc
     1921 cttgaagacg ccgcccttgc ccttcacttc cttcaaattt tcttcacgac tcgcgtccgc
     1981 cacgtcttcg ccgaataccg taatccgctc gtcgcgcgac atttcatcgt taagcgtcgc
     2041 ggccaccatc tccaccatgg tcttctgcgc gccgtgcaac tgcggttcgc cagcaaagct
     2101 cgctgaggtc ggatcaacgt ccggcgaata catgttgtcg taaatcgatt cgaccgctgg
     2161 cggatccgcg gccagtgcct gatcggtcgc ctgccgcact tcgtgatcca cctgcgcttc
     2221 aatcgcttca atctgcttct ggtccagcag tccttcgcgt accagaaaca gcgcaaattt
     2281 cgacagcggg tcgcgatgcg cctcggcttc gcgttcatcc ggcgacttgt acagtttttc
     2341 gtcgtcgctc agcgagtggg aatacggccg cgtcaccttg gcatgcacca acgccggccc
     2401 gcgccgctcg cggcaatggt tcaccgcctc ctggcaaacc cggtaacttt ccagcggatc
     2461 cgttccatcc gcttcggcaa cgtggagtcc cggaaaactt ttgaccagct tcgcaatgct
     2521 gccgcccgcc gtctgcactt ccacgggaac agaaatcgcg tagccgttgt cttccaccag
     2581 atacagcaat ggcaatttct tgatacacgc gacgttcagc gattcccaga attctccttc
     2641 gctcgtcgct ccatcgcccg acgacacgta aacaatttca tccgcagaat gcttcgccag
     2701 ttcggccagc ggcgctttct tcgcttgcgc caacgccttc ggatgctgct cgtaatagag
     2761 cgtcgcttct gccgccccca ctgcgtgcaa aaactgcgtt cccgtacacg atgacttgct
     2821 cggaatgttg aagctgggat ggccaaaatg cgagggcatt tgccgcccgc ccgaactcgg
     2881 atcgtccttt gcccccaccg cctgcagcag catctcgagc ggcgtcacgc ccagcatcag
     2941 gcacagtgcg cggtcgcggt agtacggata cacccaatcc acgccgggcc gtagaagtaa
     3001 ccccataact gcggtcacgg cttcatggcc agcgctcgaa atctggaagt aaatcttgtt
     3061 ctggcgtttg agttgaattt cccggtcgtc cagcttgcgg gaaagataca tcgtgcggta
     3121 caaccggatc agagtgtcgc ggctaaggcc accgtatttt tcgttcttgg gagcttcgtt
     3181 tttagcaact ttcactcgcg tttgaacggc cattcggtgg gaccccactc actgccaatt
     3241 gggaattgtc ggtcaaccaa cgtcaatcgg gctggcgctt caaccactgt tcccgcgcct
     3301 cccattcaaa ttataccgaa cctgaaatcg cgcatcctcg acccgccgct ccctgccctc
     3361 tgggaaatta agccgaagca gtccaatttg gcttcatcac ttatccttcc agtccctcga
     3421 atttcccttc tatctccgca aaacccgcgt cgcccgtctt caccgtctcc ggctctgcat
     3481 ccttgaaagg ctcctccgtc ggaatccgct tcagccaccg ctcctcggtc atcttctcgt
     3541 gcccggagaa cgctagccgc agatgcaatt ttaccgggcc cgtggccacc gccgccgctt
     3601 cgtccttctc ttccttttct ttgggagcct tggccttcgc gctgccgccc ttccccgcct
     3661 tgccggcctt cggaggcttt tcggcttttg cagctccttt cctggcggga gccgcttctg
     3721 ctgcgttagc accatcgcct tctggcgtcg cgcgctcgaa tttataaagc agcttcttgt
     3781 ccagccggaa cgcctgcttc catccgtcca gcctgtgccg cgcttgctgt gccttctctt
     3841 catccgtacc aaaatcaaac gtcatgtaaa tgtgggccat gagccgagtc tgcgtcccac
     3901 tccgccgcaa gtcaagttat ggcaaggaaa tgtctggagg gatttgcaag gtagcaagag
     3961 ccggagtcac cacgcaaaaa tcgcggcttc gt
//
LOCUS       DASTPM010000514         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_104638, whole genome shotgun sequence.
ACCESSION   DASTPM010000514 DASTPM010000000
VERSION     DASTPM010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104638"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VFR42_09110"
     CDS             <1..1278
                     /locus_tag="VFR42_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0369355.1"
                     /translation="PQLNAMQAQVNVSNQNLKAAEAQYTQARALLRYQRADYFPTVTV
                     DPTVTRNRYSANRQPHSPQFSGVSFTDIQIPFELSYQLDLWGRVRRTVESYREQAQAS
                     AADLAAVNLSMHSQLALFYFQARMLDAEEQLLNSTVTQYEQALELITNRFTGGLASDL
                     EVQQARTQLETTRAQAIDVGVARAQFEHALAVLMGKPPASFSLTPLPLTMPPPPIPVG
                     LPSELLERRPDIAAAERFMASANAQIGVAKAAYYPLVNLAAAGGFESGSITTLISGPS
                     IFWSAGPSAMLTIFDVGRRRAASDQAIAAYDQAVANYRQTVLTGFQQVEDNVAALRIL
                     EHEAQVQERAVTAAQKYLELAITRYKGGVTSYLEVTTAQSAALSDQLTAVTILGRRMV
                     NAVTLVQALGGGWDRSALPERPECCGKLTSNSR"
     gene            complement(1665..2366)
                     /locus_tag="VFR42_09115"
     CDS             complement(1665..2366)
                     /locus_tag="VFR42_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0369356.1"
                     /translation="MDQVKSCAAPNTVRIFLLVGNRLLHDTLVRLFRKPADVSIAGQG
                     LPSVTPAENVQKSLCHVVLTDFMPSTHPEAALSWASRAHTLLLGMDDDEDQFLAAVRA
                     GYRGYLLKDACAADVLNAVRALSRGEAICPPRLCMSLFKWIADGGHETLRADSDARPS
                     LTIRQQQLVSLVARGLTNKEIASELNLSEFTVKNHLRRIMRQVDAESRQEAVQTARAH
                     GYLLADSTGVLRQSS"
BASE COUNT          545 a          721 c          658 g          502 t
ORIGIN      
        1 ccgcaactta acgccatgca ggcacaagtc aacgtctcca accagaactt gaaagcggcg
       61 gaagcgcaat acacacaggc tcgtgcactt ctccgatatc agagggccga ttactttcca
      121 accgtgaccg tagaccccac cgtgacgcgc aaccgatatt ccgcgaatcg gcaaccgcac
      181 agcccacagt tcagtggggt cagcttcacc gacatacaaa ttcctttcga attgtcttat
      241 cagctcgacc tctggggacg cgtacgccgc accgtcgaat cctatcgcga acaggcacaa
      301 gccagcgcag ccgatctggc agcggtgaac ttgtccatgc attcccagtt agccttgttt
      361 tactttcagg cccgcatgct cgacgccgag gagcagcttc tcaattcgac cgtcacgcag
      421 tacgaacagg cattggaatt gatcaccaac cgatttaccg gagggcttgc ttccgatctg
      481 gaggttcaac aggcccgcac gcaacttgag accactcgag ctcaggcaat tgatgttgga
      541 gtcgcccgcg cccagttcga gcacgcgttg gcggttctca tggggaagcc tccggcatcg
      601 ttcagtctta ctccccttcc gctcaccatg ccgccgcctc cgattccggt gggtctaccg
      661 tcggaattgc tcgagcggcg acctgacatc gcagctgcag agcgattcat ggcctccgcg
      721 aacgcgcaga ttggtgttgc caaggcggct tattacccgc tcgttaattt agccgccgcc
      781 ggaggctttg aaagtggttc cattaccacc ctgatttccg gccctagcat tttctggtcg
      841 gctggaccat ccgcgatgct cacaatcttc gacgtaggca ggcgtcgcgc tgcgtcagac
      901 caggccatag ccgcttacga tcaagcagtc gccaactatc ggcaaaccgt gttgaccggc
      961 ttccagcagg tcgaagacaa cgtagcggca ttgcggatct tggagcacga agctcaggtc
     1021 caggaaagag ccgtcaccgc agcacagaaa tatctcgaac ttgccatcac gcggtacaag
     1081 ggtggcgtca ccagctacct ggaagtgacc acggcgcaaa gtgcggccct ttctgaccaa
     1141 cttactgcgg taactattct cggccgacga atggtgaacg cagtgacgct ggtgcaagcc
     1201 ctgggcggcg gctgggatcg ctctgccttg cccgaacgcc ccgaatgctg cggcaagctg
     1261 acgagcaatt ccaggtaacg ctctcctagc ttctgcgaga tgctgtccat ttgatccgag
     1321 ctttcaggat tttccgcgga ttctctagcg gcagtggatc gaatgaccgt tagtgttcag
     1381 gctaggaggg acattattcc ggccgaagtt gctggtgcga gtataacttc cacctagatt
     1441 ccgtttggaa cacgcgcgat atgggtctag agccctacga accgtttctt ccaccggtag
     1501 aaggtcgctc cgtaattccc actgccggca tgcatccagc accggtacgc ctacctcagc
     1561 ttgcttcaaa tcggcaacaa tctgttcagt gggggggaag cgcttctctg caccgcaaat
     1621 ctccatgccg ctcacccatg gcccgctcca gattgcgcgc cctatcacga cgattgtcgc
     1681 aaaacaccag tgctgtcggc cagcaggtaa ccgtgggcgc gggcggtttg tacggcttcc
     1741 tgccggcttt ccgcatcgac ctgtctcatg atgcgtcgaa ggtgattctt gacggtaaat
     1801 tcggaaagat tcaattcgct ggcaatctct ttgttggtca agccgcgcgc aactaacgaa
     1861 accaattgct gctgacgaat ggtcaagcta ggtctggcgt cggagtccgc tcgaagagtt
     1921 tcatggccgc catcggcgat ccatttgaac aaggacatac aaagacgtgg tggacagatc
     1981 gcctcgccgc gggaaagcgc gcggacggcg ttgagaacat cggccgcgca agcatccttt
     2041 agaagatagc ctctgtagcc cgcacggact gcggcaagaa attgatcttc gtcatcgtcc
     2101 atgccgagaa gcagggtatg ggcacgggaa gcccaagata acgcggcttc cggatgagta
     2161 cttggcatga aatccgtcag aactacgtgg cacagagatt tctgcacatt ctcagcgggg
     2221 gtaacggagg gaagcccctg gccggcgatc gaaacgtcgg caggcttacg aaacaagcgg
     2281 accagcgtgt cgtggagtaa gcgattgccg acaagcagaa agatccgcac cgtattcggg
     2341 gcggcacaac ttttgacttg atccattcaa caattacctc ctcgcggtaa aaagtttcta
     2401 gccaacgcac gcgaagaaac caaaaa
//
LOCUS       DASTPM010000515         4120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_651632, whole genome shotgun sequence.
ACCESSION   DASTPM010000515 DASTPM010000000
VERSION     DASTPM010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651632"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VFR42_09120"
     CDS             complement(<1..1472)
                     /locus_tag="VFR42_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0369357.1"
                     /translation="MKLLLLNLSPQVAQSVNAALAGQGFEILEQHSLDVEQVESSQAP
                     VLVTEASPSDLTCCGLIVQLKAREATKGIRILMIVQGGALERARALDLGADDVISSPF
                     DPAEFAARIRAEFRDREPAEELRAKLKEAQQREQLAEMAVETLSGGVVTRKRAWVLPA
                     ILALCVLTVAATFITTFSYQKTRKDTLQLKSEVARLSSGVIQQSDLLRRAEQARQALN
                     NAAPDAAARASLRAQSDELKKKMAASGGSGDADLRRQLAETQDRLKRIESDGKIAETI
                     VKTYGSSVCLLHVVVEFLDRNTGKPLLIAVDQLGKPLLDDKGMVQLDLDGNGPHLQLD
                     VFGTGFLVRKDGRLLTNHHVAEPWWGNDELKVLIENGAKPYALSYMAYFPGKAQGFEA
                     KLDRISSQADVATLRLDGTMPPQATLLDLDDHSDASIAGESVVLIGYPTGIEGILARA
                     GPEAAQQIAGTSSNVPQIVSRLAQQNLIRPTTTQGHIGDV"
     gene            1578..2411
                     /locus_tag="VFR42_09125"
     CDS             1578..2411
                     /locus_tag="VFR42_09125"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0369358.1"
                     /translation="MISAKSFAKRSARVFLRVFPSCSMISQAIAYNLFLAFFPTLLLI
                     VAMATSRWGSQTNMLDLVTDFTKYLPPGSRQIVSEFLIKRAPQAWKWAVVGWAGTLIA
                     GSQVMKLIMEGIHVIYHDLEKPGFWHRQLRGLLLLVATIAPLLVAAVLGVFGRPLRHW
                     ATRELGEAHPFHGWWPLFFPVAAFLLSLSALILIYRAARPQETSLRNVVPGAIVATLL
                     WWLADVVFGFYVRRVPYGVVYGGLAAVIGLLIWMQISAIIIFLGAAWNAEAYAVRNGR
                     T"
     gene            2758..>4120
                     /locus_tag="VFR42_09130"
     CDS             2758..>4120
                     /locus_tag="VFR42_09130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0369359.1"
                     /translation="MVTRDSSTTFKVRVNLVLVRVVVRDEHGKIVENLKKEDFQLFDN
                     RKPQTITSFNVETPLSHAVPVVAVTDHDADPDANDQLVPNLPQRFVSLFFDDIHLEMA
                     DAVTVRAAGTKILDSMGPTDRVGIYTSSGQLTQEFTADRELVKRALTQIIPHPVGNSG
                     FHDCPEVTYYQADLIENRNDSQALAVAAEDALQCAFNGDETQRAAAMTIARAASTRAL
                     ASGDTTTTYAYQHLSDALRRLSSMPGQRKMVFVSPGFILSTLLAERSDVIDRSTRAGV
                     VIDTLDARGLYTPDLGGDIANPPADSFKTVGIRSSYRVAAQSAQNEILADFAAGTGGT
                     YYRNRNDLDVALREAISAPAISYVLGFSPQNLKLNGSFHTLRVSLTGKQKFSVQARRG
                     YYAPRTLKNPEEVAKEEIQEAVFSQEEIRDLPVELQTQFFRRDATAARLSVLARVDLK
                     GLKF"
BASE COUNT          754 a         1250 c         1212 g          904 t
ORIGIN      
        1 aagacgtcgc cgatgtgacc ttgcgtcgtt gtgggacgaa tgagattttg ctgcgccaaa
       61 cgcgaaacaa tttgcggcac gttggaactg gtcccggcaa tttgctgcgc ggcttccgga
      121 ccggcgcgcg ccagaattcc ttcgatgccg gtgggatagc cgatcaagac tactgattcg
      181 ccggcgatgc tggcgtcgct gtggtcgtca agatcgagca gggtcgcttg cggcggcatg
      241 gttccgtcca gtcggagcgt tgcgacatcg gcttgcgacg aaatgcgatc cagcttggct
      301 tcaaaacctt gcgccttgcc tggaaaatac gccatgtagg aaagggcgta cggcttggcg
      361 ccattctcga tgagcacttt cagttcgtcg ttgccccacc acggctccgc gacgtgatga
      421 ttggtcagca agcggccgtc cttgcgaacc aggaagcccg ttccaaaaac gtcaagctgc
      481 agatgagggc cattgccgtc gaggtcgagt tgcaccatgc ccttgtcgtc gagcagcggt
      541 ttgcccagtt gatccacggc gatgagcagc ggcttgccgg tgttgcggtc gagaaactcc
      601 accaccacgt ggagcagaca gacgctggag ccgtaggtct tcacgatggt ttcggcaatt
      661 tttccgtcgc tttcgatccg cttgagacgg tcctgcgttt cggcgagctg gcggcgcaag
      721 tcggcatcgc cgctgcctcc gctggccgcc attttctttt tcagttcgtc ggattgcgcc
      781 cgcaaagacg cgcgcgccgc cgcatcgggc gcggcgttgt tgagcgcctg gcgtgcctgc
      841 tcggcgcggc gcagcaagtc gctctgctgg atgacgccgg agctgagccg cgcgacttcg
      901 ctcttaagtt gcagcgtgtc cttgcgcgtc ttctgatagg aaaacgttgt aatgaacgtt
      961 gccgcaaccg tcagcacgca gagcgcaaga atcgctggga gcacccaggc gcgtttgcga
     1021 gtgaccacac cgccgctcag cgtttccacg gccatttcag ccagttgttc gcgctgctgc
     1081 gcttccttta gtttcgcgcg caactcttcc gccggttcgc gatcgcgaaa ctccgcccga
     1141 atgcgagcgg cgaattccgc cgggtcgaat ggcgaggaga tgacatcgtc cgcgccaagg
     1201 tcgagggcgc gagcgcgttc cagcgctccg ccttgcacaa tcatcagaat gcgaattccc
     1261 ttggtggctt cgcgggcttt cagttgcacg atcaagccac aacaggtgag gtcggagggg
     1321 ctggcctccg ttaccagcac aggcgcctgc gaagattcga cctgctcgac atcgagagaa
     1381 tgctgttcca gaatttcgaa accctggccg gcgagtgccg cattgacgct ctgcgccacc
     1441 tgcggcgaaa gattgagcag gagaagtttc atccggaata gtttagcaga agcggtttgc
     1501 cgagttagcc ttccggtcca gacgggctcg gcgccagtat tccgcgccaa gcgcgaccgc
     1561 gtttatactg atgcgcgatg atttcagcca agtccttcgc caagcgcagc gcacgggtgt
     1621 tcctgcgggt atttccgtcg tgcagcatga tttcgcaggc catcgcgtac aatctgttcc
     1681 tggcgttttt tccgacgctg ctgctcatcg tggcgatggc caccagccgc tggggcagcc
     1741 agaccaacat gctcgatctg gtcactgact ttaccaagta tttgccgccg ggcagccgcc
     1801 agatcgtctc cgaattcctg atcaagcgcg cgccgcaagc ctggaagtgg gcagtggtcg
     1861 gctgggccgg tactttaatc gcgggttccc aggtgatgaa gctcatcatg gaaggaattc
     1921 atgtcattta tcatgacctg gaaaagccgg gtttttggca tcgacagttg cggggcttgc
     1981 tgttgctggt ggccaccatt gcaccgctgc tggttgcggc ggtacttggc gtgttcggcc
     2041 gccccctgcg gcactgggcc acccgcgagc taggcgaagc gcatcccttc cacggatggt
     2101 ggccgctgtt ctttcccgta gctgcgttcc ttctgtcgct ttccgcgctg atcctgatct
     2161 atcgtgccgc gcggccgcag gaaaccagct tgcgcaacgt tgttcccggg gcgatcgtcg
     2221 ccaccctctt gtggtggctg gctgatgtcg tctttggttt ctacgtacgc cgcgtgcctt
     2281 atggtgtagt gtatggcggt ctggccgccg tcatcggctt gctcatctgg atgcagattt
     2341 ccgcgatcat tattttcctg ggagctgcat ggaacgccga agcctatgcc gtgcgcaatg
     2401 gacgcacctg aaccttacga gatgatggct cggttcaatt gacaccgatt ttacgcgcgg
     2461 cgtatagtcg ccgtagtagc attgcttcag ggtgcccagc gtttgctcgt gctgaccggg
     2521 tcgaggcggt taggcggttg gagctatgcg gtaaacatgc gccaccagcc tctttttcgc
     2581 atttcgtcgg catctccgat ccggccttcg ctggttcttg cgctgttgct tgctttgtcg
     2641 gggtccagtt tcgcgcaaaa tccgcccgcg cccgataact cgtccaaacc tgctaccact
     2701 cagccagccg caaacacaga cactaaagcg caaggcgctg cgccttcttc acaggaggtg
     2761 gttacccgcg attcctctac cacgttcaag gtgcgcgtga acctggtgct ggttcgcgtg
     2821 gtggtgcgcg atgaacacgg caaaattgtc gaaaatctca aaaaggaaga ctttcagctt
     2881 tttgacaatc gcaagccaca aaccatcacc agtttcaacg tggaaacgcc gctgagccat
     2941 gcggttcctg tggtggccgt aaccgaccac gatgccgatc ccgacgcgaa cgatcagctg
     3001 gtacccaacc taccgcaacg gtttgtctcc ctgttttttg acgatatcca cctggagatg
     3061 gccgacgcgg taaccgtgcg cgccgccggc accaagatcc tcgattccat ggggcccacg
     3121 gatcgcgtgg gcatttacac atcctcgggg cagctcacgc aggaatttac cgccgaccgc
     3181 gaactcgtga aacgggcgct cacccagatc atcccgcacc cggtcggcaa cagcggtttt
     3241 cacgattgcc cggaggtcac ctactatcag gccgacctca tcgagaatcg caacgatagt
     3301 caggcattgg cggtggcggc ggaagatgca ttacagtgcg ctttcaatgg agatgaaaca
     3361 cagcgcgcgg ctgcgatgac catagcccgc gcggcgtcta cgcgcgcttt agccagcggc
     3421 gacaccacca ccacatatgc ctatcagcat ctttccgacg ctctgcgcag gctcagttcc
     3481 atgcccggtc agcgcaagat ggttttcgtc tcgcccggtt ttatcctttc gaccctgctg
     3541 gccgagcgta gcgacgtcat tgatcgttct actcgcgccg gcgtggtgat cgacacgctc
     3601 gatgcgcgag gcctttacac gccagacctt ggcggcgaca tcgccaaccc tcccgccgat
     3661 tctttcaaga ccgtggggat ccggtcttcc tacagggtgg ccgcgcagtc cgcgcaaaac
     3721 gaaatcctgg cggattttgc cgccggcacc ggcggcactt actatcgcaa ccgcaatgat
     3781 ctcgacgtgg cgttgcgcga agcgatctcc gccccggcca tttcttatgt cttgggtttt
     3841 tcgccgcaaa acctcaagct caatggcagc tttcacacgc tgagagtcag cctgaccggc
     3901 aagcagaaat tcagcgtgca ggcccggcgg ggctactacg ctcctcgcac gctgaaaaat
     3961 ccggaagaag ttgccaagga agagatacag gaagctgtgt tttctcagga agagatccgc
     4021 gacttgccgg tggaactgca aacgcaattc tttcggcgtg acgctaccgc ggctcgcctt
     4081 tcggtgctgg cccgcgtcga tctgaaaggg ctgaaattcc
//
LOCUS       DASTPM010000516         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1258293, whole genome shotgun sequence.
ACCESSION   DASTPM010000516 DASTPM010000000
VERSION     DASTPM010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258293"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFR42_09135"
     CDS             complement(<1..852)
                     /locus_tag="VFR42_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0369360.1"
                     /translation="MADGKVKVGIIGSQFQADIQVASYASMPHEAEVVAVASPTPGNA
                     AELAKKYNLPRVFTDYREMLKERDIEMVNICAPNALHAQMTKDIAAAGKHIVCEKPLA
                     MTIAEGEEMIDAAKRHGVLLMYGEELFFTPKYLKAKEMADAGAFGKIHLVKQSEKHFG
                     PHSPWFWDVNRSGGGALMDLGCHGIAFCYWFLGRSPIKSVYCQMGTYVHGDKTKAEDE
                     SLCILEFANGAMGLVEDSWARRGGMDDRIEVFGSEGVTYANLHMGNALPTYSENGYGY
                     AVEKAPTT"
     gene            complement(894..1508)
                     /locus_tag="VFR42_09140"
     CDS             complement(894..1508)
                     /locus_tag="VFR42_09140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU0369361.1"
                     /translation="MSLKNPIDLLQDRRQWLKTSATMLGVALVPASAVAAGEAQQFAK
                     SIAPPSHPHEDKAARFFTPEQHALVEELSETILPADSHSGGAKAAKVADYIDQTLRNT
                     HDDNQKALWREALPLMDSMCQHYTGKAFVNASPEERVAVLTVLSDNIRMTELPEVRFF
                     KELKRITVEGYYTSKIGIHDELQYKGNRILKEYVGCDDQGAATT"
     gene            complement(1517..>2311)
                     /locus_tag="VFR42_09145"
     CDS             complement(1517..>2311)
                     /locus_tag="VFR42_09145"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEU0369362.1"
                     /translation="STLESARLLLLSKSRQHPNGLGNSSGHVGHNFCEHVMGPGVAGR
                     AKNLVGKPPTLYDGRPGGFYLTRFRNLKEKHPKFIRGYGFEGGSGSAMFPRGTEVAGF
                     GASFKEEVRKNQGAYIGMGAFGEVLSRYDNYVDLDPAVKDKWGIPALRFHYKFGDNEH
                     RMVEDMKATAQEMFEAAGFEIIHVNDRILTEGWSIHELGTARMGNDPKTSVLNQFEQS
                     HDVKNLFVVDGSAFVNASCQNPTWTIMSLCWRSCDYLVDEMKKGNL"
BASE COUNT          441 a          708 c          617 g          545 t
ORIGIN      
        1 cgtggtgggc gccttttcca ccgcgtagcc atagccgttc tcgctatagg tcggcagagc
       61 gttgcccatg tgcagattcg cgtaggtcac gccctccgag ccgaagactt cgatgcggtc
      121 gtccatgccg ccgcggcgcg cccagctgtc ttccaccagg cccatggcgc cgttggcaaa
      181 ttccaggatg caaagagatt cgtcctcggc tttcgttttg tcgccgtgaa cgtaggtgcc
      241 catctggcaa tacaccgact tgatcggcga acgcccgaga aaccagtaac aaaacgcgat
      301 gccatggcag cccagatcca tcaacgcgcc gccaccggag cgattgacgt cccagaacca
      361 cggactgtgc gggccgaaat gtttttcact ctgcttgacc agatggattt tgccgaacgc
      421 gccggcatcc gccatttcct tggccttcag atatttcggc gtgaagaaga gttcttcgcc
      481 atacatgagc aagacgccgt ggcgtttcgc agcgtcgatc atttcctcgc cttcggcaat
      541 agtcatggcc agcggctttt cgcagacaat gtgtttgccg gcagcggcga tgtctttcgt
      601 catctgcgcg tgtaaggcgt tcggcgcgca gatattgacc atctcgatgt cgcgctcctt
      661 cagcatctcg cgatagtcgg tgaagacgcg cggaaggttg tactttttcg ccagctccgc
      721 ggcgttgccc ggtgttggcg aagcgaccgc caccacctcc gcttcgtgag gcatgcttgc
      781 gtaggaagcg acctgaatgt ccgcttgaaa ttgcgaaccg atgatgccga ctttcacttt
      841 tccgtctgcc atgttttctc cgccaggtat agaattccgg acttcacgtt gccttacgtc
      901 gtcgcggcgc cctgatcgtc gcagcccacg tattccttca aaatacgatt gcctttgtac
      961 tgcagttcat cgtgaatgcc gattttcgac gtgtaatagc cctcaactgt gatgcgcttc
     1021 aactctttga agaaccggac ttcggggagt tcagtcatgc gtatgttatc ggaaagcacg
     1081 gtaagcaccg cgactcgctc ctccggcgaa gcgttcacaa aggctttgcc ggtatagtgc
     1141 tggcacatgc tgtccatcaa gggcaacgcc tctctccaca gagctttctg gttgtcgtca
     1201 tgggtgttgc gcaatgtctg gtcgatgtaa tcggcgacct tcgctgcctt ggcgccaccg
     1261 gaatggctgt cggccggaag aattgtctcc gacaattctt cgaccagcgc gtgctgctcc
     1321 ggcgtgaaga agcgcgcagc cttatcttca tgagggtgcg acggcggcgc gatcgatttc
     1381 gcgaattgtt gcgcttcccc cgcggccaca gccgaagcgg gcaccagcgc cacgccgagc
     1441 atcgtcgcag aagtcttgag ccattgccgc cggtcttgca acagatcaat cggatttttg
     1501 agcgacatcg ctcctcctac aaatttcctt ttttcatttc gtccaccagg taatcgcatg
     1561 agcgccagca caacgacatg atggtccacg tcggattctg gcagctggcg ttcacaaaag
     1621 cgctgccgtc cacgacgaac aggtttttca cgtcgtgcga ttgttcgaac tggttcagca
     1681 ccgacgtttt tggatcattc cccattcgcg ccgttccgag ttcgtgtatg gaccagcctt
     1741 cggtcaagat acggtcgttg acgtgaataa tttcaaaacc ggcagcctcg aacatttcct
     1801 gcgcggtcgc cttcatgtcc tcgaccatgc ggtgttcgtt gtctccaaac ttgtagtgaa
     1861 agcgcagcgc cggaatcccc cacttgtctt tcaccgccgg gtcgagatcc acatagttat
     1921 cgtaacgcga cagcacctcg ccgaaggcgc ccatgccgat gtacgcgccc tggtttttcc
     1981 gtacttcctc cttgaacgat gcgccgaagc ccgccacttc cgtaccgcgt ggaaacatcg
     2041 ctgaaccgct gccgccttca aaaccgtagc cacggatgaa tttcgggtgc ttttctttca
     2101 aattgcgaaa ccgcgtcaga taaaaaccgc ccggtcggcc gtcatacagc gttggcggct
     2161 tgcccaccag attcttggcg cgccccgcga cgcccggtcc cataacgtgc tcgcaaaagt
     2221 tgtggcctac gtgtccgctc gaattgccca gcccgttcgg atgctgccgc gacttggaaa
     2281 gcagcagcaa tcgcgctgat tccagcgtgg a
//
LOCUS       DASTPM010000517         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_18376, whole genome shotgun sequence.
ACCESSION   DASTPM010000517 DASTPM010000000
VERSION     DASTPM010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18376"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFR42_09150"
     CDS             <1..514
                     /locus_tag="VFR42_09150"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEU0369363.1"
                     /translation="IGDKVVYPNHGVGVIEQINYGILNGKTERYYMIRIVSSGLRVMV
                     PQTNAMSVGLRSVMKTNDTSKVLGFLERGKLNCHHDWKHRFKENSERMRTGSLLEVAV
                     VLKSLCSLSRSKPLSFREKKMLERAKYLLVSEMATARNTSAELAETMVLKSLAKAKLQ
                     LPIMQEGFEQ"
     gene            complement(591..2240)
                     /locus_tag="VFR42_09155"
     CDS             complement(591..2240)
                     /locus_tag="VFR42_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU0369364.1"
                     /translation="MAARKNQPDAMLLEVFKNIFHSIAEEMGAALRRSAFSPNIKERR
                     DYSCAVYDAKGQVLAMGDHMPVHLGSMPASVAAARNALAFEPGDIALLNDPFAGGTHL
                     PDLTLVAPVFLPGALFPPGAEDQPHFFVASRAHHADIGGALAGSMGPAREIYQEGLRI
                     PPVKIFRNGKIDNDVLRLLLANVRTPIERAGDLAAQIASCRLGERRLRETVAKYTLKT
                     TDTYAAHLLDYSEKMMRAELRSLESGMYRAEDFLDSDGTNSTPLRIRVAIRVTGDSAI
                     VDFTGSASQCAGNINAVEAIAISAVYYVFRSLLREDVPATSGLLRPITVIAPSGTIVN
                     ATPPAAVAGGNVETSQRMVDTLLRALAKAAPDRIPAASQGTMNNLTFGGLDRRRGNQP
                     FTYYETIAGGCGASARCNGPSAAHSHMTNSWNTPTEVLEESYPVRVNRYAIRHNSGGA
                     GRHRGGDGIIRELEFLASVEVGLLTDRRAIGPYGLNGGHDGSTGRNTLRRKSFVKALP
                     AKCSFAAHAGDILRIETPGGGGWGNPSKKQSKHANQRKRSR"
     gene            complement(2230..4272)
                     /locus_tag="VFR42_09160"
     CDS             complement(2230..4272)
                     /locus_tag="VFR42_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0369365.1"
                     /translation="MRIATDTGGTFTDCVFVRDGKLEILKVPSQPRQPAASIASALAE
                     VGARSNGETPAEIDLVCGTTVGTNALLERRGGRIMLITTAGFEDVLEIGRQARPKLYD
                     LFFQKTDVLVPGSGRVGARERVAADGRIVAALTSGEIRRLARKVQQTRPDAVAICFLF
                     SFRNPRHETALAKALRRAGCLVSVSHEILAEFREFERTSTTVVNAYLAPVMTSYLREA
                     EARARSAWRQKRSKPKVSVRVMQSNGGVIAAQRAADEPVRTVLSGPAGGVLGAAYAAR
                     LAGLDQVLTFDMGGTSTDVALLSGETQITTEARVAGLPVAVPMLEIHTVGAGGGSIAR
                     FDRGGALRVGPESAGADPGPVCYGRGEQPTVTDAHAVLGHFGDRGLLNGAFPLDIARA
                     QDELNKRKGRCASVEAFAQGIIGVSNSVMERALRLVSIERGHDPRDYTLVAFGGAGGL
                     HACDLAEALDLRGVLLPVFPGALSALGILRANVVRDFSRTVLLRVAQSAEALDEARRY
                     LVQMRRSAQGFLRNQGFAPAQQRFDDRLDVRYVGQAYELSVAATPRFVDDFHRGHEQA
                     YGHAETQRSVEIVNVRCRAVGRSPEIALPRIAKARAGAAPPARTWTVSYWSGKNREVP
                     LYDRQTLRAGHTFSGPAIVAEYSATSLVPDGWRARVDAYGQIHLAMSGKSARRHGR"
     gene            complement(4344..5471)
                     /gene="tsaD"
                     /locus_tag="VFR42_09165"
     CDS             complement(4344..5471)
                     /gene="tsaD"
                     /locus_tag="VFR42_09165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU0369366.1"
                     /translation="MSIRILGIESSCDETAAAVVADGREILSSVVASQIDIHRKYGGV
                     VPELASREHLRRIVPVVREALENAKLRFEDLDAIGVTHGPGLVGSLLVGITYGKTLAE
                     SLGKPLVPVNHLEGHIHAVFLDAHKACVTPVLPAVCLIVSGGHTVLYEVHPKDGMNGD
                     AGALWSYRKIGQTRDDAAGEAYDKVSKLLGLGYPGGPILDRLAAAAGDAPAPVKFGPT
                     KIKGNAYDFSFSGLKTAVLYHLRENADYTPEILGREAALARGERKFDQLLPLCSRKTL
                     GLVKEFQNAVVRELVERTSKAAEELGVPTILVSGGVAANRQLRATFEERAAALGRKPH
                     FPSLALSTDNAAMVAAAAYPRLKNHLLADTSLNASASLPLA"
     gene            5593..>6283
                     /locus_tag="VFR42_09170"
     CDS             5593..>6283
                     /locus_tag="VFR42_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEU0369367.1"
                     /translation="MTISSPSHASAAPIKKVRKAVLPAAGLGTRFLPATKAQPKEMLT
                     VVDKPQIQYVVEECVVSGIEHIIIVTGKGKNSIEDHFDYNPTLERFLEEKGKKDQAEL
                     VRRISDMVQVSYTRQKEPLGLGHAVLVAKDLVGDEPFAVLLGDVLIPGDNPATKQLVA
                     VYEATGVGAIAVEEVPHEKTQQYGIVAGDPAPQAQFGERLLRIRDLVEKPKPADAPSN
                     LAITGRYVLPPA"
BASE COUNT         1333 a         1846 c         1872 g         1232 t
ORIGIN      
        1 aattggcgac aaggtcgttt acccgaatca tggcgttggg gtcatcgagc agatcaatta
       61 cggaatcctg aacggaaaaa cagaacgcta ctacatgatt cgtatcgtgt cgagcggcct
      121 gcgcgtgatg gtgccccaaa cgaatgcgat gagcgtcgga ttgcggtccg tgatgaaaac
      181 gaacgacacc agcaaagtgc tgggttttct ggagcgcgga aagttgaatt gccatcacga
      241 ctggaagcat cgcttcaagg aaaactccga aagaatgcgt accggttccc tgttagaagt
      301 tgcagtggtg ttaaagagcc tttgttcctt gagccgcagc aagcccctct ccttccgcga
      361 aaagaagatg ctggaacgcg ccaagtattt gctggtcagc gagatggcga cggcgcggaa
      421 tacttcggcc gaattggcgg agacgatggt gctgaagtcg ctggcgaagg cgaaactgca
      481 gttaccgatc atgcaggaag ggttcgagca gtaggccaaa aagttttgca aaaattttac
      541 agggataggc cgcgtgcctg tccctttttg tttttggtgc aggatgattg tcagcgtgaa
      601 cgcttacgct gattcgcgtg tttgctttgc ttttttgaag gattacccca cccaccgccc
      661 cctggggttt cgatccggag aatgtcgccc gcatgtgcgg cgaacgaaca cttggcagga
      721 agtgctttca cgaagctttt gcgcctcagc gtgttcctgc ccgtcgagcc atcgtggccc
      781 ccgttcagac cgtacggacc gattgcgcgg cgatcagtca gcaggccaac ttcgacggag
      841 gcgaggaatt cgagttcgcg gatgataccg tcaccgccgc gatgcctgcc agcgccccct
      901 gagttatgac ggatcgcgta gcgatttacg cgaacgggat agctctcttc cagaacctcg
      961 gtcggcgtat tccacgaatt ggtcatgtgc gagtgagccg cggacggtcc attgcaacgt
     1021 gcagaagcgc cgcagccacc cgcaatggtt tcgtagtagg tgaaaggctg gttgccgcgg
     1081 cggcgatcga gtcccccaaa agtgaggtta ttcatggtgc cctggctggc cgccgggatg
     1141 cgatcaggag ccgctttcgc aagagcgcgc aggagcgtgt ccaccattcg ctgcgaggtt
     1201 tcgacgttgc caccggcgac ggcagcaggc ggagtggcgt taacgatggt tccagaaggg
     1261 gcgatgaccg taatcggacg gagcaagcct gaggttgcgg gaacgtcctc gcgcaagagc
     1321 gaacggaaaa cgtaatagac ggcggaaatg gcgatggctt cgaccgcgtt gatatttccg
     1381 gcgcactgcg aggctgaacc ggtgaagtcg acgatggccg aatcgcccgt gacgcgaatg
     1441 gcaacgcgga tgcgcagtgg cgtggagttt gtgccgtcgc tgtccagaaa atcctccgct
     1501 cggtacatcc cggactcaag tgaccgcaac tcggcgcgca tcattttttc ggaataatcc
     1561 aacaagtgcg cggcataggt atccgtcgtt ttgagggtgt acttggcgac tgtttcgcgc
     1621 aggcgccttt cgcctaagcg acaggatgca atttgtgccg ccaggtctcc tgctctttca
     1681 ataggagtgc gcacgttcgc gagcagcagg cgcaacacgt cgttatcgat ttttccgttg
     1741 cgaaaaatct tgaccggcgg aatgcgcaag ccttcctgat agatttctcg cgcaggtccc
     1801 atcgatccgg cgagcgcgcc gccgatgtcg gcgtggtgag cgcgactggc gacaaagaaa
     1861 tgcggctggt cttcggcgcc aggcggaaaa agcgcgccag gcagaaagac gggcgcgact
     1921 agcgtgagat cgggaagatg cgtgccgccg gcgaacggat cgttgagcaa ggcgatgtcg
     1981 ccgggttcga aggccagcgc atttctggcg gcggcaaccg acgccggcat cgagcccaag
     2041 tgtacgggca tgtggtcgcc catcgccagt acttggcctt tcgcgtcata aaccgcacag
     2101 gaataatcgc ggcgttcttt aatgttcggc gaaaaggcgg agcggcggag cgccgcgccc
     2161 atttcttctg caatggaatg aaagatgttc ttgaaaactt ccagcagcat ggcatcgggc
     2221 tgattttttc tagcggccat ggcgcctcgc actcttgccc gacatcgcta aatggatctg
     2281 tccgtaggcg tcgactcgcg cccgccaacc atctggcacc aaagaagtgg cgctgtactc
     2341 cgcgacgatt gctgggccag agaaggtatg accggcgcgc agtgtttgac gatcgtaaag
     2401 cggcacttcc ctgttctttc cagaccagta gctcactgtc caggtccgtg cgggaggagc
     2461 cgcaccagct cgcgctttcg caatgcgagg cagggcgatt tctggcgaac gtccaaccgc
     2521 ccggcagcgg acatttacga tttctacaga acgctgcgtc tccgcgtgac cgtacgcctg
     2581 ctcgtgccca cggtgaaagt cgtcaacgaa gcgcggcgtt gccgccacgc tgagttcata
     2641 ggcttgtccc acgtagcgaa catccagccg gtcgtcgaaa cgttgctgcg ccggagcaaa
     2701 cccctggttc cgcagaaatc cttgtgcact gcgtcgcatc tggaccaggt aacgccgcgc
     2761 ctcatcgaga gcttcggcgg actgagctac gcggagcaga acggtgcgag aaaaatcgcg
     2821 caccacgttg gcgcgcaaga tacctagagc ggacagggct cccgggaaaa ccggaagcag
     2881 aacaccacgg aggtctagag cttcagccag atcacaagcg tgcaagccac cggcgccacc
     2941 gaacgcgaca agcgtgtagt cacgtggatc gtggccgcgt tcgatggaga cgagccgcaa
     3001 cgcgcgctcc ataacggaat tggagacgcc gatgattccc tgcgcgaagg cttcgacgct
     3061 ggcgcatctt cctttgcgtt tattgagttc gtcctgcgca cgtgcaatgt ccagcgggaa
     3121 cgctccgttc agcaggccgc ggtcaccgaa atgtccgagg acggcgtgcg cgtcggtcac
     3181 cgtcggttgc tctccgcggc catagcagac cggcccggga tccgcgcccg cgctttccgg
     3241 gccgacgcgt agtgcgccac ctctatcgaa gcgggcgata gagccgccgc ccgctccgac
     3301 cgtgtgaatc tcgagcatcg gcaccgcgac cggaaggccg gcaacgcgag cctcggtggt
     3361 gatttgcgtt tcgcccgaca gaagtgcgac atcggtggaa gtaccgccca tatcaaaggt
     3421 aagcacttgg tcgagtccag ccagtctggc tgcgtaagcg gcgccgagaa ctcccccggc
     3481 cggaccggaa agaacggttc gcaccggttc gtccgcggcg cgttgcgccg caatgacgcc
     3541 gccgttcgac tgcatcactc ggacactgac cttcggcttc gagcgtttct gtctccatgc
     3601 ggaacgcgcg cgggcctccg cttcgcgcag gtacgaggtc atcaccggcg cgagatacgc
     3661 gtttacaacc gtagtggaag tgcgttcgaa ctcgcggaat tccgcaagga tttcgtgcga
     3721 aacggagacg agacacccgg cacggcgtaa tgctttcgcc agtgccgttt catggcgagg
     3781 attgcgaaaa gaaaaaagaa agcagatggc gactgcgtcg gggcgagttt gctgaacttt
     3841 gcgcgccaag cgacgaattt ctcccgaggt gagcgccgca acaatcctgc cgtcggcggc
     3901 gacacgctcc cgagcgccga cacgtccgct cccgggcaca agcacatcgg tcttctgaaa
     3961 gaacaaatcg tagagtttcg gccgcgcctg ccgaccgatt tccagcacat cctcaaaacc
     4021 cgcggtcgtg atcagcatga tgcgaccgcc gcggcgttcc agcaatgcat tcgtgccgac
     4081 cgtggttccg cagacgaggt cgatttcggc gggcgtctcg ccgttggacc gggctccaac
     4141 ttcagccaga gcactggcga tggacgcggc tggctgtctc ggctgcgacg gaaccttcag
     4201 aatttcaagt ttaccgtcgc gcacaaagac gcaatccgtg aacgtccctc ccgtgtcggt
     4261 cgcaattcgc atgaggcggg cattttaacc tgagcgagcc ccagggtccg aattcagcat
     4321 ggctttcctg agagcttgcg cctttaggcc aacggcagag atgccgacgc gttgagcgaa
     4381 gtgtcggcga gaaggtgatt tttcagccgt ggataggccg cagcggcaac catcgcggca
     4441 ttgtcggtgg agagcgccaa gcttgggaaa tgcggcttgc ggccaagagc agccgctcgt
     4501 tcttcaaagg tagcgcgcag ctgccgattg gcagcaactc cgcccgaaac aagaatcgtc
     4561 ggcacgccca attcttcggc ggccttgctg gtgcgctcca ccagttctcg caccactgca
     4621 ttctggaatt ccttgaccag gccgagcgtt ttgcggctgc acaacggcaa gagttgatcg
     4681 aacttgcgtt cgccgcgcgc aagggcggct tcgcgtccca gaatttccgg cgtgtaatca
     4741 gcgttttccc gcaaatgata cagcacagcc gttttcagac cgctaaagct gaaatcgtat
     4801 gcatttccct taatttttgt agggccaaat tttaccggag cgggcgcgtc acccgcggct
     4861 gccgccagcc ggtccaaaat tggtccgccc gggtaaccca gaccgagcag cttggaaact
     4921 ttgtcatatg cctcgccggc ggcgtcatcg cgggtctgcc cgatttttcg gtaggaccag
     4981 agcgcgcccg cgtcgccgtt catgccatct ttagggtgca cttcgtaaag caccgtgtgg
     5041 cctccagaaa ctattagaca gactgcggga agaacaggcg tcacgcaggc tttatgcgcg
     5101 tccagaaaca cagcatgaat gtggccttcc aggtgattta ccggcactag cggcttgccg
     5161 aggctttccg ccaacgtttt cccgtaagta attcccacca gcagcgaccc caccaagccc
     5221 ggcccgtggg tcacgccaat cgcatccaag tcttcgaagc gcagcttggc gttttccagt
     5281 gcctcgcgga ccacgggaac aattctgcgc agatgctcgc gcgaagccag ttccgggact
     5341 acgccgccat atttgcggtg tatatcaatc tgcgaagcga cgacgctgga aagaatttcg
     5401 cggccgtccg ccacgaccgc ggcagccgtc tcatcgcacg aagattcgat gcccaggatg
     5461 cgaatgctca tttcaagcgg aaacgtcccc ggatctatgc taacacgcgc atcgtggacg
     5521 ctgactttcg ctccacgcac gttacagcaa gaatccgatt gaaaatttcg ccagcttctt
     5581 taccctgaat tcatgacgat ctcttcccct tcccacgcat ctgccgcgcc catcaaaaaa
     5641 gttcgcaagg cagtccttcc tgcagcagga ttgggtacgc gattcctgcc cgcaaccaaa
     5701 gcgcagccca aagaaatgtt gacggtcgtg gacaagccgc agattcaata cgtcgtcgaa
     5761 gaatgcgtgg tcagcggcat cgaacacatc atcatcgtca ccggcaaagg caaaaattcc
     5821 atcgaggatc atttcgatta caacccgact ctggaacgtt ttctggaaga aaaaggcaaa
     5881 aaggatcagg ccgagctggt tcgtcgcatc agcgacatgg tgcaagtcag ctacacgcgc
     5941 cagaaagagc cgctgggact tggtcatgcg gtgctggtcg caaaagatct ggtcggcgac
     6001 gagcctttcg ccgtgcttct cggcgacgtg ctgattcccg gcgacaatcc ggcaaccaaa
     6061 caattggttg ccgtatacga agccacaggc gtaggcgcca tcgccgtgga agaagtcccg
     6121 catgagaaaa cccagcaata tggaattgtc gcgggagatc cggctccgca ggctcagttt
     6181 ggcgaacgat tgttacgcat tcgcgatctc gtggaaaagc ccaagcctgc tgacgctcca
     6241 tccaacctag cgatcaccgg acgctacgtc ttgcctcccg cca
//
LOCUS       DASTPM010000518         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_940909, whole genome shotgun sequence.
ACCESSION   DASTPM010000518 DASTPM010000000
VERSION     DASTPM010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940909"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..939)
                     /gene="rpe"
                     /locus_tag="VFR42_09175"
     CDS             complement(247..939)
                     /gene="rpe"
                     /locus_tag="VFR42_09175"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU0369368.1"
                     /translation="MPSARKIELAPSILSANFANLAAHAKQALEGGGTLLHVDIMDGH
                     FVPNLTIGPPVVASLRKALPDVIFDCHLMIENPDEFIPEFAKAGATWISVHQEACIHL
                     DRTLHHIADHGCKPGVVLNPATPVETLTDVLDLVHHVLVMSVNPGFGGQRFIRNSLNK
                     MKQLVRMRDERRLDFRVEVDGGVAVDTVTDIVRAGAELLVAGNAVFGHGDANLNAREL
                     LDAALAATLTRA"
     gene            complement(1102..2148)
                     /locus_tag="VFR42_09180"
     CDS             complement(1102..2148)
                     /locus_tag="VFR42_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0369369.1"
                     /translation="MIDVGLIGFGLGGKAFHAPVIAAVEELRLAAVLQRHENTAAEIY
                     PGVKIVRTVEEMLSNESIELVAISTPNDTHFSYVKACLEAGRDVVVDKPFTTTLAEAK
                     ELAGLARQRGRLLTVYQERRLDGDFETLKKLIANGELGRPVHYEETFDRCRAQVRDSW
                     KEKVGPGGGVFWDLAPHLIDHALVLFGEPHALLADLRLERDGARNVDAFDVTFYYANG
                     FRAVMSSTTLAPVARPHFRVRGTKAEFIKQGLDPQEALLRAGQPMGGPSWGLEKPEEW
                     GTLSVIEGDQKIAPRKIESARGDYRAFYENVRDVMLGKAKPLVTLQDALRVMYALELS
                     DSSSAQRAVLAWGM"
     gene            2258..>2695
                     /locus_tag="VFR42_09185"
     CDS             2258..>2695
                     /locus_tag="VFR42_09185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0369370.1"
                     /translation="MMPTETQLPQVRQSSVQNTIFVLLCGGFIVNGIVITFIAPILPI
                     FMAKWGLDDSRAGLFSLVQFSASLVGVLISSALVSSKGFKPAIVLGLAMLGIGFALLN
                     APTFPLALLASGLYGLGYGFATPGTNLWVAESYGDRRASALNVA"
BASE COUNT          521 a          960 c          642 g          572 t
ORIGIN      
        1 cgtgaatact gcgcacatca ggagaattac agtggctgca aatccgaaac gtttctccca
       61 tcctgcgctt cgtcaaggct agcttcgcaa gcgtacagaa aaagagtgcg gggtactacc
      121 tttttgcaaa tccggaactg catcttcagt tcctcggcat tacgaattgt tcaggaattc
      181 tgcggaagaa ttgttctcga ccactcgtct aaacagagta gcggcggagc cgtcgcgaag
      241 ggccgcttac gcccgcgtca atgtcgccgc cagcgccgcg tccaacagct ccctggcgtt
      301 cagattcgcg tcgccgtgcc caaacacggc attccctgct accaacaatt ccgctcccgc
      361 gcgcacaatg tccgtcaccg tatccaccgc cacgccgccg tccacttcga cccgaaaatc
      421 cagtcgccgt tcgtcgcgca tccgcaccag ctgtttcatc ttgttcaggg aattgcgtat
      481 gaaccgttgc ccgccaaatc caggattcac gctcatcacc agcacatgat gcaccagatc
      541 cagcacgtcc gtcagcgtct ccaccggcgt cgcaggattc agcaccactc ccggcttgca
      601 cccatgatcc gcaatgtggt gcagcgtccg gtccaggtgt atgcacgctt cctgatgcac
      661 cgaaatccat gtcgcgcccg ccttcgcaaa ttccggaata aattcgtcgg gattctcaat
      721 catcaggtgg caatcaaaaa tcacgtcggg caaagccttg cgtagcgagg ccaccaccgg
      781 cggcccgatc gtgaggttcg gcacaaagtg cccgtccatg atgtccacgt gcagcagcgt
      841 tccgccgcct tccagtgcct gcttggcgtg cgctgccagg ttggcaaaat tcgccgacaa
      901 tatcgagggg gccagctcaa tttttcgcgc gcttggcaaa gttctgctcc tcttcctagc
      961 ttacccgatt ctggcccggg ctcatcattc cgccacaagc ccggtccaca ggggggcgga
     1021 gctccagctc cggcataaat gcttggcgaa tgagtgggct tcagcccctg aggaattggt
     1081 tcttgaacgc acttacgccc tctacatccc ccacgccagc acggcgcgct gcgcgctgct
     1141 cgaatcgctc aactccagcg cgtacatcac ccgcagcgca tcctgcagcg tgaccagtgg
     1201 cttcgccttg cccagcatca cgtcgcgcac attctcataa aacgcccgat agtcgccgcg
     1261 cgccgattcg atcttacgcg gcgcgatctt ttggtcgcct tcaattaccg acagcgttcc
     1321 ccactcctca ggtttttcca gtccccacga tggcccgccc atcggctgac ccgcgcgcaa
     1381 aagcgcttcc tgcgggtcga gcccctgctt gatgaattcc gcttttgtcc cgcgcacccg
     1441 aaaatgcggc cgcgccaccg gcgccagcgt ggtcgaggac atcaccgcgc gaaatccgtt
     1501 cgcgtagtaa aacgtcacgt caaacgcatc cacattgcgc gcaccatcgc gctccagcct
     1561 gagatccgcc agcagcgcgt gcggctcgcc aaacagcacc agcgcatggt caatcaaatg
     1621 cggcgccaga tcccagaaca ctccgccgcc cggccccact ttttccttcc aagaatcgcg
     1681 cacttgcgcc cggcaccggt caaaggtctc ctcgtaatgc accggccgcc ccagttctcc
     1741 attcgcaatc aatttcttca gcgtttcaaa atcgccgtcc agccgccgct cttggtatac
     1801 cgtcagcagc cgtccccgct ggcgcgccag ccccgccaat tctttcgctt ccgcgagcgt
     1861 cgtcgtgaac ggtttgtcca ccaccacgtc gcggcccgct tccaggcaag ctttcacata
     1921 cgaaaagtgc gtatcgtttg gagtcgaaat cgccaccagc tcgatcgact cattcgacag
     1981 catttcctca acggtcctca cgatcttcac gccgggataa atttccgccg ccgtgttttc
     2041 atgccgctgc agcaccgcgg ccagccgcaa ctcctccacc gccgcaatca ccggcgcatg
     2101 aaacgccttc cctcctaagc caaacccaat caaccccaca tcgatcattc gcgcgttctc
     2161 cgttgtcaaa cagacttgtc attccgagcg aagcgtttaa tctctctgcc tctgccgatg
     2221 cccatttgcg cctcaacctc ggctccggca aaatgccatg atgcccaccg aaacgcaact
     2281 tcctcaggtc cggcaaagct ccgtccaaaa cacaattttc gtgctgctct gcggcggctt
     2341 catcgtcaac ggcatcgtaa tcaccttcat cgctccgatt cttccgatct tcatggccaa
     2401 atgggggctc gacgacagcc gcgccggact tttctccctc gttcaatttt ccgcttcgct
     2461 cgtgggagtt ctcatatcca gcgcactggt ttcctccaaa ggtttcaaac ccgccatcgt
     2521 cctcgggctg gccatgctgg gaatcggctt cgcgctgctc aacgccccca cctttccgct
     2581 cgccttgctg gccagcggcc tttacggtct tggttacggt ttcgccactc cgggcaccaa
     2641 cctctgggtt gccgaaagtt atggcgaccg ccgcgcatcc gctttgaacg tcgca
//
LOCUS       DASTPM010000519         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_710567, whole genome shotgun sequence.
ACCESSION   DASTPM010000519 DASTPM010000000
VERSION     DASTPM010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710567"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="VFR42_09190"
     CDS             complement(<1..1603)
                     /locus_tag="VFR42_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU0369371.1"
                     /translation="MSAGTQTQTSARPVPPHTVPPKKDLSGLMPYLRRYTGGIVVGLL
                     TVLLMGIIGNVIPLATGVMTDTLAGNPVPFEHATATHNRVMLPGLSGSALSRSIPYYA
                     PNSRRTLGIYCLIVVVCVVLKGLLSFTARWKLIGVSRDIEFDIRNDLLKRLLLLEPEF
                     YVRNRTGELMSRATNDLNAVRMVLGPGIMYSATTIVTMIFAIAVMLALSPSLTLWVLL
                     PAPIVAVAVWFFGRTIHDLYEKIQAALATLTARVQENLSGVRIIRAYAQEEAEKKVFD
                     EPNLEYVSRNIKLIRTWSMFMPSLQALIGTTFLIVLWQGGHQLLRGQISLGALISFNA
                     YLTLLVWPMIALGWVTNIFQRGAASMGRLNYILTAQASIDDSHATIAADRKPDGAIEF
                     RGLTFAYPTNLSGGDSAKAKTNGSGNGKNGSHPVLRDIDLKIPAGSTLAIVGPTGSGK
                     TTLAALIARLWEAPDGQLYLDDKPLREWPLASLRKSIGFVPQDTYLFGETIGGNIAFG
                     LPELKDEMVQEAAAIANLDSDVNDFP"
     gene            complement(1600..2211)
                     /locus_tag="VFR42_09195"
     CDS             complement(1600..2211)
                     /locus_tag="VFR42_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369372.1"
                     /translation="MPKTLCSSLLLTLLSSSLLFSGCGNSPAPKPAEQAEAPKPAEPA
                     IPPDLQSAAEGSLGSETTVLAYGDLTKTGTQQILIANVLPKTPKDNVTGTIVSRAAIV
                     EKQGTNWVQIFLCDEHLKNSKGYLGMTPIEPVTAWRIQHEQDPMKGLTVYLTPVKGMP
                     DAHVLPIGVQWNPKTKRYQSLDRSYQQFLTESPALSNVRSRLQ"
     gene            complement(2323..2910)
                     /locus_tag="VFR42_09200"
     CDS             complement(2323..2910)
                     /locus_tag="VFR42_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369373.1"
                     /translation="MKRGWTRFWASVSLAALGFAGVTAGAPSPRADPNSLKRNELRLA
                     ALRPGRDTIARARSLYGNPKSGAKGDTTFSWTTDLRSERLTVTTDSQGLIEEIRAERR
                     DDVVIMDGDPLGSPWRTGKNLAIGDLAGRVEKLYGKPDSRSPSTKGGQRLELLYYAFD
                     WAGPDVPQVMEVVCTVGKDGQPERVVEITLAASSL"
     gene            complement(2873..3553)
                     /locus_tag="VFR42_09205"
     CDS             complement(2873..3553)
                     /locus_tag="VFR42_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369374.1"
                     /translation="MANRAYLRVWTRDFSNETMIAQFVRFLATVPLSEARLSFDQLNV
                     QAVDPTETPLAEWDFREGTFGAPEVAAIAAQHINEDTAYIVEAQWDLWTFDNETLKWL
                     LGPQPLELICRGTLYDGSSGTGEGNFSVDLGFEHLFTGHAGLLAPGAASNPFDSSDHP
                     VEKTFRQWMSVEANRKEYAARTRENIQKLFNWIESVERALPVERNELSSEGEENFEAR
                     LDAILGQR"
BASE COUNT          767 a         1156 c         1041 g          788 t
ORIGIN      
        1 tgggaaaatc gttgacgtcg ctatccaggt tggcgatggc tgccgcttcc tgcaccatct
       61 cgtctttcag ttccggcaaa ccaaacgcga tattgccgcc aatcgtctcg ccgaaaagat
      121 aggtgtcctg gggcacgaac ccgatcgatt tgcgcagcga agccagcggc cattcgcgca
      181 acggcttgtc gtccagatac agttgtccgt ccggcgcctc ccataaacgc gcgatcagcg
      241 ccgccagcgt ggtctttccg ctgcccgtcg gtcccacaat cgcaagggtc gatcccgccg
      301 gaattttcaa atcaatgtca cgcaaaaccg gatgcgaacc gtttttaccg ttgccgctgc
      361 catttgtttt cgcttttgcg gaatcgccgc ccgacaaatt cgtcggatag gcgaacgtca
      421 atccgcgaaa ctcgattgcg ccgtcgggct tgcggtctgc cgcaatggtg gcgtgcgaat
      481 cgtcaatcga cgcctgagcg gtcaaaatat agttcagccg gcccatggac gcggcaccgc
      541 gctgaaaaat attggtcacc cagcccagcg caatcatcgg ccagaccagg agggtcaaat
      601 acgcgttgaa ggaaatcagc gcgcccagtg aaatttgccc gcgcaacagt tgatgtccgc
      661 cttgccacag cacgatcaga aacgttgtcc cgatcagcgc ctgcaacgac ggcatgaaca
      721 tgctccaggt gcggatcagc ttgatgttgc gggaaacgta ttccaggttc ggctcgtcga
      781 acaccttttt ctcggcttct tcctgggcgt aagcacggat aatccgaacg ccggacaagt
      841 tttcctgcac gcgcgcggtc agcgtagcca gcgccgcctg gattttttcg tacagatcgt
      901 gaatggtgcg cccaaagaac cacaccgcga ccgccacgat cggcgccggc agcagcaccc
      961 acaaggtcag tgacggcgac agcgccagca tcaccgcaat cgcgaaaatc atggtcacaa
     1021 tcgtagtcgc gctatacatg atgcccggcc cgagcaccat gcgcaccgcg ttcaagtcgt
     1081 tcgttgcacg ggacatcaac tcgccggtac gattgcgcac ataaaactcc ggctccagca
     1141 gcagcaggcg cttcagcaaa tcgttgcgaa tgtcgaattc gatgtcgcgc gagacgccga
     1201 tcaacttcca gcgcgcggta aacgatagca gtcccttgag caccacgcag acaacaacaa
     1261 tcaaacaata aatgcccaat gtccgccggc tgttcggcgc gtagtaagga atggaccggc
     1321 tcaacgccga cccgctcaag cccggcaaca tcacgcgatt gtgcgtggct gtcgcatgtt
     1381 caaaggggac cgggttcccc gccagcgtat cggtcatgac tccggtcgcc agcggaatca
     1441 cgttgccgat aattcccatc agcaacacgg tcagcagccc gacgacgatt ccaccggtgt
     1501 agcgccgcaa atacggcatc agcccgctca aatctttttt gggcggaaca gtatggggcg
     1561 gaacgggccg cgcactggtt tgcgtttggg tccctgcgct cactgcagtc gcgacctcac
     1621 attcgacaac gccggcgatt cggtcagaaa ttgctgatac gaccggtcca gcgactggta
     1681 gcgcttggtc ttggggttcc actgcacgcc gatcggcaaa acgtgcgcat ccggcatgcc
     1741 ttttactggc gtcaaataca ccgtcagtcc tttcatcgga tcctgctcat gctggatccg
     1801 ccacgccgtg accggctcga tcggcgtcat gcccaaatag cctttggaat tcttcaggtg
     1861 ctcgtcgcaa agaaaaatct gcacccagtt ggtgccttgc ttttccacga ttgccgcgcg
     1921 cgaaacgatg gttccggtaa cgttatcttt cggtgttttc ggcagcacgt tcgcgatcag
     1981 gatttgctgc gttccggtct tcgtcaggtc accataagcg agcacggtcg tttccgagcc
     2041 caacgaacct tccgcggcgg actgaaggtc aggcggaatc gccggctcgg cgggctttgg
     2101 cgcctccgcc tgttcggccg gtttcggcgc gggcgaattt ccacatccag agaagagcaa
     2161 ggaagacgac agcaaggtca atagcaagga actgcagagg gtcttgggca cggagagcat
     2221 caccttacca gacgaacatc gcgcaacatt gtaagcgtct attggcatga tttgacgtga
     2281 ctttgactgg cccagggcca tcagcgaacc atcgcagacc ttttaaagac tagatgcggc
     2341 cagcgtgatt tcgaccacac gctccggctg gccgtctttt ccgacggtgc agactacttc
     2401 catcacctgc ggcacatccg ggcctgccca atcgaaagcg tagtacagca attctagcct
     2461 ctggccgccc ttcgtgctag gactccgcga gtccggcttg ccgtaaagtt tctccacgcg
     2521 accggcgagg tctccgattg ccagattctt tcccgtgcgc cacggactac ccagcgggtc
     2581 gccgtccatg atgaccacgt cgtcgcgacg ttcggctcga atttcctcga tcaatccttg
     2641 agaatcggtc gttacggtaa gcctctcgct gcgaaggtcc gtggtccagg aaaatgtggt
     2701 atcgcccttc gctccgcttt tcgggttgcc gtaaaggctg cgcgccctgg ctatcgtgtc
     2761 acggccgggc cgcagggcgg ccaggcgcag ttcattccgc ttcaggctgt ttggatcggc
     2821 gcgcggcgag ggggcgccgg cagtgactcc ggcaaatccc aacgcggcga gactaacgct
     2881 ggcccagaat cgcgtccaac cgcgcttcaa aattttcctc cccctcgctg gacaattcgt
     2941 tgcgttcgac gggcaatgcc cgttccaccg actcaatcca gttgaacagt ttttggatat
     3001 tttcccgcgt tctggctgcg tattctttgc ggttggcttc gaccgacatc cattgccgga
     3061 aggttttctc cactggatgg tctgagctgt cgaatggatt cgacgccgcg cccggcgcca
     3121 gcaatcccgc gtggccggtg aacagatgct cgaatcccaa gtccacgctg aaattgccct
     3181 cgccggttcc ggagctgcca tcgtataacg tcccgcggca aatcaattcg agcggctgag
     3241 gtcccagaag ccacttcagc gtctcgttgt cgaacgtcca caagtcccat tgcgcttcca
     3301 cgatatacgc cgtgtcttcg ttgatgtgtt gcgcagcgat ggccgccact tccggcgcgc
     3361 caaacgtgcc ttcgcggaaa tcccactctg cgagcggcgt ttccgtcgga tccacagcct
     3421 gcacgttgag ttgatcaaag ctgaggcgtg cttcagaaag cggcactgtc gccagaaatc
     3481 gcacaaactg cgcgatcatt gtttcgttag agaaatcccg tgtccacacc cgcaaataag
     3541 cccggttcgc catggaaggg gaattataca gccgcctgcg tcgcgccgtg aaacgggggt
     3601 agaggcagct cggacttcag tttgcgcggt tgtattcagt tcgatgacaa aagtggccgg
     3661 tgtcggaaag cctacgagag atgggccgtt caccctttat cggcggccgg cttggaaaac
     3721 tccgcggtga gttcctgcaa gcgttttttc ag
//
LOCUS       DASTPM010000520         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_651672, whole genome shotgun sequence.
ACCESSION   DASTPM010000520 DASTPM010000000
VERSION     DASTPM010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651672"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFR42_09210"
     CDS             complement(<1..746)
                     /locus_tag="VFR42_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0369375.1"
                     /translation="MERTPGPDDYLLIDRLREDATSGRMDLSSILSEATHAARYFTDA
                     TGAALALWSQGVVICRARSGDTAPPLGAKLDVDSGISGECLRSGRSQRCHDSETDPRV
                     DAELCREMGIRSLAAVPIRGGQGVIGILETFSDKPNAFSDAHITLLKKLAKIAATGRE
                     QSATVPVRSIAQVPTQSPVTPAPEVPPKPESPHPWATRDWFAFLPARMQGEEGQPLRL
                     AAAAALLLLLAVFGWALSRSRGNRNDSGNAA"
     gene            1098..2129
                     /locus_tag="VFR42_09215"
     CDS             1098..2129
                     /locus_tag="VFR42_09215"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369376.1"
                     /translation="MEASWTTSLPATIIAQAPCRADLAGGTIDLWPLYLFHPGALTLN
                     FAVNILTTCRITRLKGKRIHLRSLDTRKEETFASFEELRLAQRFRLPLAARLLQFFAP
                     KEGLLMETDSESPAGAGISGSSALMVASSAALARFTDRHLTAEQIRVIAQNIEAQVIS
                     VPTGCQDYYPALYGGVSAIHLNADGIHRESVPMPPEEIESRFVLAYTGAPRQSGINNW
                     EVFKAHINGDKRVFRNFERIAEIARLMYQGLLHADWDEVARLLREEWKLRRTNAPGIS
                     TPLIDKLIAVAGKNGGRAAKVCGAGGGGCVIFLVDKGAASRVATVIGDNGGRVLPLQV
                     ARDGLRLVT"
BASE COUNT          494 a          691 c          727 g          488 t
ORIGIN      
        1 gcggcgttcc cagagtcgtt gcggtttcca cgagagcggc tcagggccca tccgaaaacc
       61 gccagcaaaa ggagtaacgc ggcggcggcg gcgagccgta aaggctgacc ttcttctccc
      121 tgcatgcgcg cgggaagaaa cgcaaaccag tcccgtgtcg cccatgggtg cggagattct
      181 ggctttggcg ggacctcggg cgcaggcgtg accggagact gcgtcgggac ttgtgcgatc
      241 gacctcaccg gcacggtagc ggattgttcc cgtccggtgg cggcaatctt tgccagtttc
      301 ttcagcagcg tgatgtgtgc gtccgagaac gcattcggct tgtcggaaaa agtttcgaga
      361 atgccaatga cgccttggcc tccgcgaatg ggaacggccg caagcgagcg aattcccatc
      421 tcgcggcaga gttccgcatc cacgcgtgga tcggtctctg agtcgtgaca gcgctgcgag
      481 cggccgctcc gcaaacactc gcccgagatt cccgagtcca catcgagctt ggcgcccaga
      541 ggtggcgctg tgtcgccgga gcgggctcgg caaatgacaa ccccttggct ccacaacgca
      601 agtgccgcac ccgtcgcgtc ggtgaaatac cgcgcggcgt gcgtggcttc ggagaggatg
      661 ctgcttaggt ccatccgccc cgaggtcgcg tcttcgcgga ggcggtcgat cagtaagtag
      721 tcgtcgggcc cgggggttcg ctccaactgc gctcgtgctt catcttcaga cggagagggg
      781 actccaggat ccgggggacc tggggaggta ggcctaaccc atggatctgc catacgcaaa
      841 ctcctagcga acggccagag tgattgataa attcatgaga ctgccgatga acttcctctc
      901 gcgaatatcc aataaatgtg aagtgttgtc aacggaagat cgcgaagagc gtgcgaaaaa
      961 gcacgctccg cgaggtctct cacaagcttc actgcgtgga atgcaggact ggcgcggcgt
     1021 tatcgacatg gtaccgtgag taacctgatt acccggaagg tgtcgtagcc cggcgcgcgg
     1081 cgtgatacaa actctgcatg gaagcgtcct ggacgacgtc tttgcctgcc accatcatcg
     1141 cacaagcgcc gtgccgcgcg gatcttgccg gcggaactat cgatctctgg cctttgtatc
     1201 tgttccatcc aggagcactg acccttaatt tcgcggtcaa cattctgacc acgtgccgca
     1261 tcacgcgcct gaagggcaag cgcattcatt tgcgttcgct cgataccagg aaagaagaaa
     1321 cattcgcgag ctttgaggaa ttgcggcttg cgcagcggtt ccgccttccg ttagcggcgc
     1381 gcttgctgca attttttgca ccaaaggaag gattgctgat ggagaccgac tcggagtccc
     1441 cggcgggagc gggcatttcc ggatcgtcgg cactgatggt cgcaagttcc gcggcgctgg
     1501 cgcgcttcac cgatcgccac ctaacagctg agcaaattcg agtcatcgca cagaacatcg
     1561 aggcgcaagt catcagcgtt cccaccggat gccaggacta ctatcccgca ctctacggag
     1621 gagtgagcgc aattcacctg aacgcggacg gaatccaccg tgagtccgta ccaatgcctc
     1681 ccgaggaaat tgaatcgcgc ttcgtactcg cgtacaccgg tgcgccgcgc cagtcgggta
     1741 ttaacaattg ggaagtcttt aaggcgcaca tcaacggcga caaacgcgtg ttccggaatt
     1801 tcgagcgcat cgccgaaatc gcaaggctca tgtatcaagg tctgttgcac gcggactggg
     1861 atgaagtcgc gaggcttttg cgcgaagagt ggaagttgcg acgtaccaat gcccccggaa
     1921 tcagcacgcc gctcatcgac aagttaattg ccgttgccgg gaagaacggc ggccgcgccg
     1981 caaaggtttg tggcgccggc ggcggtggct gtgtcatctt cctcgtcgac aagggcgctg
     2041 ccagccgggt ggccaccgtg atcggcgaca acggcggaag agtgttaccg ctgcaggtcg
     2101 cgagggacgg gctgaggttg gtgacatgag cgcgcgcccg caatatcgat gttcaaatca
     2161 accgcgtctt tcgtgatgac cctcacatac ttttttcaaa gtgaagtgtc actctacgga
     2221 tggtgggtgc gcgcacgcgt gtaactaagg gccgcagctc gatccattgt tgtgggaaac
     2281 atggcagcgc ataccacgtg agttctgttc tttgacaact cagtccttct cccggaatat
     2341 tttccatata agtccagcaa actcaaagat tttgagctgg actcgagttc tctctctttt
//
LOCUS       DASTPM010000521         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_479645, whole genome shotgun sequence.
ACCESSION   DASTPM010000521 DASTPM010000000
VERSION     DASTPM010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479645"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFR42_09220"
     CDS             complement(<1..706)
                     /locus_tag="VFR42_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369377.1"
                     /translation="MRRRALALLLFAAVAGRIPAAAQEKETSKQAVEKRLRDVQKEKS
                     SLSVEKAIEALSVASSFGQVAISPDGKKVAWVESWRDKGGAETGNSAIYATTIDGKAP
                     ARRITASRAERAERDIAWAPDSRRMAFLSDAEKPGQLQLYLEGVAGQPAKRLTNVKGF
                     LAAPKFSPDGKTIAVLFTENAARAAGPLVAETPETGEIKDAFFEQRLALVDILTGKLR
                     QVSPADTYVYEYDWAPD"
     gene            887..2182
                     /locus_tag="VFR42_09225"
     CDS             887..2182
                     /locus_tag="VFR42_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0369378.1"
                     /translation="MSDRRAFLSSFGAVAAALALPGPKLLYQEMSHQAPPALPAAALY
                     DKDEEAYWTAIRKQFLIPDDEVYLNNGTVGSSPLPVLHAIMKCYHDAESLAQHDPEDY
                     PIWGYAAWNDFRDPLAKFVGCNRDEIALLRNATEANSYIANGLDLKPGDEVLMTDQEH
                     PGGEHPWNLRAKRYGITVKKVTLPKPVPDVQMILNLFSEAISSRTRVIFFSHITTVTG
                     AVLPAKQLCALARSKGILSAVDGAHVPGMMKLDLHDLGCDMYSSSPHKWLQATKGTGF
                     LYVRDEVIDRLWNTVATEGWDDKKIRAERFQRIGSSNVPALCGLRAAIDFANAIGIDR
                     IEKRHRANADYILAEMSKRGALSWTSPDPALRCGITTVNVAPYTMPETENWLWSQKKI
                     RIRGGAPSKIRLSTPYYILRKDIDRFLASYDEYRKTKSA"
BASE COUNT          469 a          811 c          527 g          457 t
ORIGIN      
        1 tatccggcgc ccagtcatat tcataaacat aggtatcggc cggcgaaact tgccgcagct
       61 tgccggtcag aatgtccacc agcgccaatc gctgttcgaa aaacgcatcc ttgatctcgc
      121 cggtctctgg agtttccgcc accagcggtc cggccgctcg cgccgcattt tctgtaaaca
      181 acaccgcgat cgtctttcca tcgggcgaga atttgggcgc agccagaaag ccttttacgt
      241 tggtcagccg cttcgccggt tgccccgcca ctccttccag atacagttgc aactgccccg
      301 gtttttccgc gtctgacaaa aacgccatgc ggcggctgtc tggcgcccac gcaatgtcgc
      361 gctccgctcg ttcagctctc gaagccgtga tcctccgcgc tggcgctttt ccatcaatcg
      421 tagtcgcgta aattgccgag ttcccggtct cggcaccgcc tttgtcgcgc cagctctcta
      481 cccacgccac ttttttcccg tccggtgaaa ttgccacttg gccgaacgaa ctggctacag
      541 ataacgcctc aatcgccttt tccaccgaca gcgacgactt ttccttctgc acgtcgcgta
      601 gccgcttctc gaccgcctgt ttcgacgtct ctttttcctg cgccgccgca gggatccggc
      661 ccgcaacggc ggcgaacagc aacagcgcaa gagcgcgacg cctcatctcg taatctcccg
      721 aaaatccgca caagaatagc gttcgtacga tagtgcctcc cgcttagcac cgcaagcgat
      781 acagcggcgc acctgtcgca cctgaacccc cgccgctatt gttcccgcaa ctcgaaacgc
      841 ataaaatccg ctgcgactct tgccaaagac gcccgtggag gcacgcatgt ctgatcgtcg
      901 cgcatttcta tcttctttcg gagccgtagc agccgctctt gctctgcctg gtccaaagct
      961 cctctaccag gaaatgtcgc atcaggctcc gccagcgctt cccgccgcgg ccctctacga
     1021 caaagacgaa gaagcctact ggactgccat ccgcaaacaa ttcctcattc cagacgacga
     1081 agtctacctg aacaacggca ccgtcggctc ctcgccgctc cccgtcctgc acgccatcat
     1141 gaagtgttat cacgacgcgg aaagcctcgc ccaacacgat cccgaggact accccatctg
     1201 gggttacgcc gcctggaacg atttccgcga cccgctagcc aaattcgtcg gctgcaatcg
     1261 tgacgaaatc gctctccttc gcaacgctac cgaagccaac agctacattg ccaacggtct
     1321 cgacctcaaa cccggcgacg aagtcctgat gaccgaccag gagcatcccg gcggggaaca
     1381 tccttggaac ctgcgcgcca agcgctacgg catcacggtg aaaaaagtca ctctgcccaa
     1441 gcccgttccc gacgtccaga tgattctcaa tcttttcagc gaagccatct cctcgcgcac
     1501 ccgcgtcatt ttcttcagtc acatcactac cgtcaccggc gcagtccttc ccgcgaaaca
     1561 actctgcgct ctggctcgtt cgaaaggaat tctctccgcc gttgatggcg cccacgttcc
     1621 cggcatgatg aagctcgatc ttcacgacct cggctgcgac atgtactctt ccagcccgca
     1681 caaatggctc caagccacca aaggcaccgg cttcctttac gtccgcgatg aagtcatcga
     1741 tcgcctctgg aacaccgtcg ccaccgaagg ctgggacgac aaaaaaatcc gcgccgaacg
     1801 tttccagcgc atcggcagtt ccaacgtccc tgccctttgc ggcctgcgcg ccgccatcga
     1861 tttcgccaac gccatcggca tcgaccgtat cgaaaagcgc caccgcgcca acgccgacta
     1921 catcctcgcg gaaatgagta agcgcggcgc tctttcctgg acttctcccg atcctgcgct
     1981 ccgctgcggc atcaccaccg tcaatgtcgc gccctacacc atgcccgaaa ccgaaaactg
     2041 gctctggtcg caaaagaaaa tccgtatccg cggcggcgcc ccctccaaaa tccgcctttc
     2101 cactccttat tacatccttc gcaaagacat cgaccgcttc ctcgcctcct acgacgaata
     2161 ccgaaaaacc aaatccgcat agtttcgggg gcgaggatgc accaggacaa gacatccgcc
     2221 gtgcggcttt taccaagtga gaggtggtct cggtgctatc gcgg
//
LOCUS       DASTPM010000522         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_651682, whole genome shotgun sequence.
ACCESSION   DASTPM010000522 DASTPM010000000
VERSION     DASTPM010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651682"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1030)
                     /locus_tag="VFR42_09230"
     CDS             complement(53..1030)
                     /locus_tag="VFR42_09230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU0369379.1"
                     /translation="MPKAKIFITHPLYPEAQALLQSVCDCEFWSKQERPPKEEFLRRL
                     KDKEGLVCLLTERVGDDTLRAAPKLRIVSNVAVGYDNVDVPACTRRGVLVTNTPGVLD
                     ETTADFAWALMLAVARRVVEADQYVRAGNWQGWNFDQYCGTDVWGKSLGIVGFGRIGQ
                     AVARRAQGFGMKIIYNSRKRVGEDIEKQLTAEHRDMNALLAESDFISLHVPLNGDTRQ
                     LFDAPKFFRMKPTAFLINKSRGPVVDEAALVHALESKKIAGAGLDVFEQEPFVHPGLK
                     RPNVVLAPHLGSASTETRGRMALTAAQNIAAFFQGQRPPNPVNPELLKS"
     gene            1174..2607
                     /locus_tag="VFR42_09235"
     CDS             1174..2607
                     /locus_tag="VFR42_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0369380.1"
                     /translation="MNKEIIAKLEGIVGARNVLSRAEDLVLYEYDGSVEKARPEAVVF
                     PSSTADVVAIVKLAVKHDVPIVGRGAGTGLSGGALARGGGIMLAFGRMNKILEMDLEN
                     RRAVVQPGVVNFDLTRAVEQAGFYFAPDPSSQKSCTIGGNVSENSGGPHTLAYGVTSS
                     HVTGLELVLPNGEVKRIGGKLSDPLGYDLCGLLVGSEGTLALVTEVVTKLSKKSEAIE
                     TLLAIFETVDDATETVAELTARAITPAACEMMDGWTLGAIEDYVHAGFPRDCGAVLLI
                     EIEGIAEAVAGQAQAIDEVCKLHHAREVRTAKDDKERELLWKGRKTAFGALGRLAPTN
                     YVLDAVIPRSKLPQALRHVVKLGKECGLQVGNIFHAGDGNLHPLVLFDPRKPEEFREA
                     VRVSEEIVRYCVSIGGALTGEHGVGMEKAELMTLMFSEADFALMRRVHAQFNPTGLLN
                     PGKVFPMGKGCGETRVRTSPTHLGAPA"
     gene            2604..3992
                     /locus_tag="VFR42_09240"
     CDS             2604..3992
                     /locus_tag="VFR42_09240"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0369381.1"
                     /translation="MSVAEPTSTTARLEKVIGAEHLFTDAGRLAEYAIDAIAPKTVAR
                     PENAVQTAEIVRTAVEEKLGVIPCGARSAIGVGAPPTDFDVALDMTRLTGIASYDPGD
                     LTISVNAGMRLSDLAQALNEKKQFLPLEVPFFERATVGGAIAAGLDSPLRQFYGAPRD
                     FLIGAEFVDGTGAQAKSGGRVVKNVTGYDFHKLLNGSLGTLAVITRLNFRTYPVPVAR
                     RGFLASFTDDLGALRFAKEIAGSALTPIVVEILSPESAGLLFAQYAPFASLCAAQGKQ
                     EWTVCVGFEGSGDVCERYARELARMAREAAAQNAVAMHDEPFAALLEILREAPATMRD
                     LAADAVVLRFAALPSQLADLLRAVRSFAGSSWMRAPVLVRSGSVVYMALLPRTGDETA
                     LKQVEYFWKSVGSLRGKLEFRGSLVFCPARWKAALNVWAHVDFNVEMERRVKKAFDPN
                     AVFARGRFVGGM"
     gene            3997..>4837
                     /locus_tag="VFR42_09245"
     CDS             3997..>4837
                     /locus_tag="VFR42_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU0369382.1"
                     /translation="MSETQDLRTPAIPGLQPIYEDYARCVHCGLCLNHCPTYKLWHLE
                     ADSPRGRIFQMIRVKDGEAPVNSTFVEHIDKCLDCRACETACPSGVEYGKLVEFARAR
                     IAKEYKRPFFSRLGRNFAYRWLLPQPGRIAFFATLLRIYQNWGLQTLARKSGVLLGMG
                     LAEREALLPSVDDQFFFRRLGKTFPAIGETRARVAFFGGCVAQVSFAELNEATIRVLT
                     ANGCEVTVPRQQVCCGALASHAGDREAARSVARRNLRAFGGDEFDVILTNAAGCGSTL
                     KEYS"
BASE COUNT          942 a         1097 c         1807 g          991 t
ORIGIN      
        1 ttattgtgcc tccgagtgtc gtccgagcgt gcgagtagcg aagagctcgc gctcaggatt
       61 tgaggagttc gggattgacg gggttgggag ggcgctggcc ctggaagaag gcggcaatgt
      121 tctgagcggc tgtgagggcc atgcggccgc gggtttcggt ggaggcggag ccgaggtgcg
      181 gggcgagaac aacgttgggg cgcttgaggc cggggtgaac gaagggctct tgttcgaaga
      241 cgtcgaggcc tgcgccggcg atttttttgg attcgagagc atggacaagc gcggcttcgt
      301 ctacaaccgg gccgcgcgac ttgttgatga ggaatgcggt tggcttcatg cggaagaatt
      361 tgggcgcgtc gaagagctgg cgggtgtcgc cgttaagagg gacatggagt gagatgaagt
      421 cggattcggc gagcagcgca ttcatatcgc gatgttcggc ggtaagctgc ttttcgatgt
      481 cctcgccgac gcgtttgcgg ctgttgtaga tgattttcat accaaaacct tgtgcgcggc
      541 gggcgactgc ctgaccgatg cggccaaaac cgacgatgcc taaagatttt ccccagacat
      601 cggtgccgca gtactgatcg aaattccagc cctgccaatt ccctgcccgg acatattgat
      661 cggcctccac cacgcgacga gcgaccgcga gcatgagcgc ccaagcgaag tcggcggtgg
      721 tttcgtcgag cacgccggga gtgttggtaa cgaggacgcc gcggcgagtg caggcgggga
      781 cgtccacatt gtcgtagcca acagcgacgt tggagacgat gcgaagtttt ggagcggcac
      841 ggagtgtatc gtcgccgacg cgctcggtga gcaggcagac gagaccctct ttgtccttga
      901 ggcggcgaag aaattcttct ttgggaggac gctcctgctt ggaccagaac tcgcagtcgc
      961 agacagactg gaggagggct tgggcttcgg gatagagcgg gtgagtgatg aaaattttgg
     1021 ctttcggcat gggtggctcg tgcgtgtgat tgggaaaata aggtagcaca ggcggagcgc
     1081 gagattgcgg catttgcggg aggccttgcg aattactata gtgagcgtga ttggggactg
     1141 gggactggtg actccggagg tggcagggca atcttgaata aggaaatcat cgcgaagctg
     1201 gaaggaattg ttggtgcgcg gaacgtgctt tcgcgggccg aggacctggt gctgtatgag
     1261 tatgacggat cggtggagaa agcgaggccg gaggcggtgg tgtttccgtc gagcacggcg
     1321 gatgtggtgg cgattgtgaa gctggcggta aagcacgacg tgccgattgt ggggcgaggg
     1381 gcggggacgg gcttgtctgg cggggcgctg gcgcgcggcg gcgggatcat gctggcgttc
     1441 gggcgcatga acaagatatt agagatggat cttgagaacc ggcgggcggt agtgcagccg
     1501 ggagtagtga attttgattt gacgcgcgcg gtggagcaag cgggatttta ttttgcgccg
     1561 gacccgtcga gccagaaatc gtgcacgatc ggcggcaatg tgagcgagaa ctcgggcgga
     1621 ccgcacacgc tggcgtatgg agtgacgagc agtcacgtga ccgggctgga gctggtgctg
     1681 ccgaacggcg aggtgaaacg gattggcggg aagctgagcg atccgctggg ctacgactta
     1741 tgcggattgc tggtgggcag cgaagggacg ctagcactgg taacggaggt tgtgacgaag
     1801 ctttcgaaaa agtccgaggc catcgagacg ctgctagcga tcttcgagac tgtggatgat
     1861 gcgacggaga cggtggcgga gttgacggca cgagcgatta cgccggccgc ttgcgaaatg
     1921 atggacgggt ggacgctcgg cgcgattgag gattacgtgc atgcggggtt tccgcgggat
     1981 tgcggagccg tgctactcat tgagattgaa ggtatcgcgg aggcagtggc gggacaagcg
     2041 caggcgatcg acgaagtttg caagctgcat catgcgcgag aagttcgcac ggcaaaggat
     2101 gacaaggagc gcgagctgtt gtggaagggg cgcaaaactg cttttggggc gctggggcga
     2161 cttgcaccga cgaactatgt gctggatgcg gtgataccgc gttcgaagct cccgcaagca
     2221 cttcggcacg tagtgaagct ggggaaagag tgcgggctgc aggtggggaa tatttttcat
     2281 gcgggcgatg ggaatttgca cccactggtg ctgtttgatc cgcggaagcc ggaggagttt
     2341 cgcgaggcgg tacgggtgtc ggaagagatt gtacggtatt gcgtttcgat tggcggagcg
     2401 ctgaccggcg agcacggcgt gggaatggaa aaagcggagc tgatgacgct gatgttttcc
     2461 gaggcggatt ttgcgctgat gcggcgcgtg catgcgcaat tcaatccgac gggactgctg
     2521 aatccgggga aagtttttcc gatgggcaag gggtgcggcg agacgcgagt gcgcactagc
     2581 ccgacgcatt tgggagcgcc ggcgtgagcg ttgcggagcc cacctcgacg acggcgcgtc
     2641 tggaaaaagt gataggtgcc gagcatctat tcaccgacgc ggggcgactg gcggaatatg
     2701 cgattgatgc tattgcgccg aagacggtgg cgcgaccgga gaacgcggtg cagacggcgg
     2761 agattgttcg aactgcggtg gaagaaaaat tgggagtgat tccatgcggg gctcgatcgg
     2821 cgattggagt tggggcgccg ccaaccgact ttgacgtggc gctggacatg acccggctta
     2881 cgggaattgc cagctacgac cccggcgatt tgacgataag cgtgaacgcg gggatgcggc
     2941 tgtcggacct ggcgcaggcg ctgaacgaaa agaaacagtt cttgccgctg gaagtgccgt
     3001 tttttgaaag ggctacggtg ggcggggcga tagcggcggg actagattcc ccgctaaggc
     3061 aattttatgg agcgcctcgg gattttctga ttggcgccga gtttgtggat gggacgggag
     3121 cgcaggcgaa gagtggcggg cgcgtggtga aaaatgtaac cgggtacgat tttcacaagc
     3181 tgctaaacgg gtcgctcgga acactggccg tgattacgcg gctgaatttt cggacatatc
     3241 cggtgccggt ggcaagaagg ggatttttgg cgtcgtttac ggacgacttg ggcgcgctga
     3301 ggtttgcgaa ggagatcgca gggtcagctt tgacgccgat tgtggtggag atcctttcac
     3361 cggagtcggc gggattgctg tttgcgcaat acgcaccctt cgcatcgcta tgcgcggccc
     3421 agggcaaaca agagtggacg gtgtgcgtgg gattcgaagg aagtggcgat gtttgcgagc
     3481 ggtatgcgcg ggagttggcc cgaatggcac gcgaagctgc ggcgcagaac gcggtggcga
     3541 tgcacgatga gccattcgca gcgctgctgg agattttgcg cgaggcgccg gcgacgatgc
     3601 gcgatctagc agccgacgcg gtggtgctgc gattcgcggc gttgccgtcg caattagcgg
     3661 acttgttgcg ggcggtccgg agctttgcgg gatcgtcgtg gatgagagcg ccggtgctgg
     3721 tgcgtagcgg ttcggtggtt tacatggcgc tgcttccgcg aaccggagat gagacggcgc
     3781 tgaagcaggt ggagtatttc tggaaaagcg tgggatcgct gcgcggcaaa ttggagtttc
     3841 gtgggtcgct ggtgttttgc ccggcaagat ggaaagctgc gttaaacgta tgggcgcatg
     3901 tggactttaa tgtggagatg gagcggcgcg tgaaaaaggc atttgatccg aacgctgtat
     3961 tcgctcgcgg gcggtttgtg ggaggaatgt gacgacgtga gcgagacgca agaccttcga
     4021 acgccagcaa tccctgggct ccagccaatt tacgaagatt atgcgcgatg cgtgcactgc
     4081 gggctgtgcc tgaaccactg tcctacctat aaattgtggc atctggaagc ggattcgccg
     4141 cgcggacgga tattccagat gattcgcgtg aaggacggcg aagccccggt caactcgaca
     4201 ttcgtcgagc acatcgacaa atgtctggat tgccgggcgt gcgagacggc gtgtccctcc
     4261 ggagtcgagt acggaaagct ggtggagttt gcgcgcgcac gaattgctaa ggaatacaaa
     4321 cggccttttt tttcgcggct ggggcggaat tttgcttacc ggtggctgct gccccagcca
     4381 gggcggatcg cattttttgc gactctgctg cggatttatc agaattgggg cttgcaaact
     4441 ctggcgcgca agagcggggt gctacttggg atggggctgg cggagcgcga ggcattgctg
     4501 ccaagcgtgg atgatcagtt tttcttccgg cggttaggca agacttttcc tgctatcggc
     4561 gagaccaggg cgcgggtggc gttttttggc ggatgtgtgg cacaggtgtc gttcgcggaa
     4621 ctaaatgagg ctacgatccg ggtgctgacg gcgaatggtt gtgaggtgac cgtgccgcga
     4681 cagcaggtat gttgcggagc gctggcgtca cacgccgggg atcgcgaggc ggcgcggagc
     4741 gttgcccgac gaaatttgcg cgcatttggc ggagatgaat ttgacgtcat ccttaccaac
     4801 gcggcgggtt gcgggtcgac attgaaggag tactcgc
//
LOCUS       DASTPM010000523         5463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_508444, whole genome shotgun sequence.
ACCESSION   DASTPM010000523 DASTPM010000000
VERSION     DASTPM010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508444"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VFR42_09250"
     CDS             complement(<1..709)
                     /locus_tag="VFR42_09250"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU0369383.1"
                     /translation="MSTFSRESLAVLLAAAVLGFAGGCSSSHVSDDALAKDVQDKVSM
                     DPATKDSPVSVTAKDGKVVLRGTVKDAATQKRMEELAKGTPGATGVDDQTAVVGATGP
                     DATAGTASDAGGSAPAAAAAPAPIVVPAGTDITVTVDQKLSSKTSQTGQAFMASVAQP
                     VTVDGRTAIPKGSSVSGKVLRAKAKGKIKGEGELAITLTSVTIHGKNYTLATGTLDST
                     VKGKGKRTAVTTGGGAAG"
     gene            1007..1393
                     /locus_tag="VFR42_09255"
     CDS             1007..1393
                     /locus_tag="VFR42_09255"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU0369384.1"
                     /translation="MSNAYLLKTEPSEYSFADLQKDKKTVWDGVHNPVALRHLGAMKP
                     GDKLIVYETGENKSAVGTASVVAVKDPNPKNPQIEIAAGKPLGKPVSLAEIKANKIFA
                     ASPLVRMGRLSVVPLTPEQYKSLTGS"
     gene            complement(1483..4704)
                     /locus_tag="VFR42_09260"
     CDS             complement(1483..4704)
                     /locus_tag="VFR42_09260"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369385.1"
                     /translation="MNFSLAMRWCVAVVAIAASARSTTAQTYDQKFFQEMRWRCIGPF
                     RAGRTVAITGVSGQPSLFYMAAVNGGVWKSNDAGNTWTPIFDEAGESGSVGAIAVAPS
                     NPNVIYVGSGEGLQRPDLAVGDGIYKSADAGKTWTHLGLRDAQQITAIVVDPKDPERV
                     YVAAQGHPYGPNVERGVFRSTDGGKSFEKVLYKDENIGAAELVMDPANPQILYAALWA
                     ARVAPWEVRSGASIYIAGSGLYKSTDRGSTWKPLTKGLPTGEEKLGRIGVAIAPSNPN
                     RLYASVEAEKGAGVYRSNDGGESWTLVNGDHRIGGRGPGAMGIGVAPDNPDVLYVLNT
                     ASWKSTDGGKTFVGWKGAPGGDDYQRIWIHPTDGKIIALSADQGAVISLNGGETWSSW
                     YNQPTAQFYHVTTDNRFPYWVYGAQQESGSAATISRSDYGEITFRDWRLLGVEEYGTI
                     AVDPLDNNIVYGGRVSRTNLALGEVQDVSPEPVRRGEFRYDRTLPLVFSPTDPHKLYF
                     AANVVFETSDGGKNWKVISPDLARPSYENPPNIGAFAAGDPEKGKHRGVVYALAPSYL
                     EKGTIWAGTDDGLIQLTRDGGKSWTNVTPPALTPWSKVSIIEASRTSAGAAYAAVNRF
                     RLDDLHPHIYRTRDFGQSWQEIVAGLPENAPVNVVREDPVKKGLLFAGTEASVFVSFD
                     DGDHWQSLQLNLPHTSMRDLTIHGDDLVVGTHGRSFWILDDLTPLRQLNESVSASAPY
                     LFVPQDAVRIHWNRNPDTPLPPEEPGGKNPPDGAIVDYFLPGGSGFPVTLEILDAAGK
                     PVRKYSSTDTALPISDIASKHPIPMYWVRENKILSAEAGMHRFVWDVRYAAPKSLSHG
                     YPISAIPHDTPLEPQGPWALPGRYTVKLTVSGKTLMQPLVLKMDPRVKSGPAELAQQF
                     SLQKSAAAGMDESFEILEELRSVRGQIADRIGKANGELKAKLIDFDQKAAALEGAAVP
                     GFFGTPLNGKQPENFSTLNQRFGRILAIADSADVAPTSPTDAVARELEGALKESSDGW
                     NDLKTSQINALNQLLNGEKLPKIDPNKRGGEAPSSEADGDDDP"
     gene            complement(4815..5174)
                     /locus_tag="VFR42_09265"
     CDS             complement(4815..5174)
                     /locus_tag="VFR42_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369386.1"
                     /translation="MKRSSSRVLVAAAFVLAIWFFTPLFLKARQTRAPKLPGFAANQA
                     LLNGPPVVYKSEKLAREANDQLELRLDVQRLYALSTELKDEVDHANPDATLSLTVLKR
                     TQDIEKLAKQIRDRAKR"
BASE COUNT         1120 a         1744 c         1402 g         1197 t
ORIGIN      
        1 cgccagccgc gccaccaccc gtggtcaccg cagtcctttt acccttccct ttcacggtgc
       61 tgtcgagcgt tcccgtcgct aacgtgtaat tcttcccgtg aatggtgacg ctggtcagcg
      121 taatcgccag ttcgccttcg cccttgatct ttcctttcgc cttcgcgcgc agcaccttgc
      181 cactcacgct cgaacctttg ggaatagcgg tcctcccatc aacagttacc ggctgcgcca
      241 cggatgccat aaacgcttgc cccgtttggc tcgttttcga gctcagcttt tggtcgacgg
      301 tcaccgttat gtcagtgccg gccggcacca caattggcgc tggcgcagcc gcagcggctg
      361 gcgcactgcc gccggcatca gacgcagtac ccgcagtcgc atcgggccca gttgcaccca
      421 ctacggcagt ctggtcgtcc acgccggtcg cgcccggagt ccccttcgcg agttcctcca
      481 ttctcttctg cgtcgcggcg tcctttacag tgccgcgcag gaccaccttg ccgtccttcg
      541 cggttacgct caccggggaa tcctttgtcg ccggatccat agataccttg tcctgcacat
      601 ccttggcgag cgcatcatcg ctgacatgcg atgacgagca acctcccgcg aaaccaagca
      661 ctgcggcagc cagcaacact gccaaacttt cccttgaaaa cgtgctcata acgcctcctc
      721 agtaaaccgc ctccgtcaat gcgttccgtg cagatgtcac ccgcaaaacc acgcaccagc
      781 ttgtttcgta gcacttaagc cagctctgac acggctccat ccaataacaa cgccgaccat
      841 tttgctttaa aatttaattg gattcgcgag cggctggtac tcagcattac agcctttccc
      901 acctgccaag tcaagttctt gcggttaact tttcgtgagg cgcagtgcca ctttaagaca
      961 atttcaaccg ttgactatcc caggcggtag tgctcaaact aacgccatgt ccaatgccta
     1021 tttgctcaaa acagaaccca gtgaatattc cttcgccgac ctgcaaaagg acaaaaaaac
     1081 cgtctgggac ggcgtgcaca atccggtcgc actccggcat ctcggcgcta tgaagcctgg
     1141 cgacaaactg atcgtctatg agaccggtga gaacaaatcg gcggtaggca cggcttccgt
     1201 tgttgctgtg aaggatccta atcccaagaa tccgcagatt gaaatcgcag caggaaagcc
     1261 gctcggcaaa ccggtttccc tagcggaaat caaggccaac aagatttttg ctgcttcgcc
     1321 gctggtaaga atgggccgac tctctgtcgt tcccctgacc ccagaacagt acaaatcgct
     1381 caccggcagc tgagctcgct gacctattag aattgtgtcg ccgtcgtttc cttcgcttac
     1441 tcgttcgcgg gcattttcag aacagaaaat ccgattctct aattacggat catcgtcccc
     1501 atccgcctcg gacgacggag cttctccgcc gcgcttgttg ggatcgatct ttggaagctt
     1561 ttcgccgttg agcagctgat tcagcgcatt gatctggctg gtcttcaggt cgttccatcc
     1621 gtcgctgctt tctttcagag cgccctccaa ttcgcgtgcc accgcgtccg tcggcgacgt
     1681 cggcgcaacg tcagcgctat cggcaatcgc cagaatgcgt ccgaaacgct gattgagggt
     1741 cgagaaattc tccggctgct ttccattcaa cggtgtgccg aagaatcccg gaaccgctgc
     1801 cccttccaag gccgccgctt tctggtcgaa gtcaatcagc ttggctttca gttccccgtt
     1861 ggccttgccg atccggtccg caatttgtcc gcgcaccgaa cggagctctt caagaatctc
     1921 aaagctttcg tccattccgg ccgccgcgga tttttgcaat gagaactgct gagccagttc
     1981 cgctggcccg ctcttcacac gtggatccat cttcaacacg agcggctgca tcaacgtctt
     2041 gccgctcacg gtgagcttca ccgtataccg gcccggcaac gcccacggtc cctgcggctc
     2101 gagcggtgtg tcgtgcggaa tcgcggaaat gggatacccg tggcttagcg acttgggagc
     2161 ggcgtagcga acgtcccaca caaaccgatg catcccggct tccgccgaca aaatcttgtt
     2221 ttcgcgcacc caatacatcg gaatcggatg cttcgacgca atatcggaaa ttggcagcgc
     2281 cgtgtcggtg ctggaatatt tgcgcaccgg tttgcccgca gcatccagaa tttcgagcgt
     2341 caccgggaaa cccgaaccgc ccggaagaaa ataatcgacg atggcgccgt ctggcggatt
     2401 cttgcctccc ggctcttccg gtggcagcgg agtatccgga ttgcggttcc agtgaatgcg
     2461 caccgcatct tgcggcacaa acagatacgg cgcagaagca gaaacgcttt cgtttagctg
     2521 cctcagcggc gtaaggtcat ccagaatcca gaacgagcgg ccgtgcgtcc cgacaaccaa
     2581 atcgtcgccg tgaatcgtca aatcgcgcat cgaagtgtgc ggcaggttga gctgcagcga
     2641 ttgccaatgg tcgccgtcat cgaatgatac aaacacactc gcttccgtcc ccgcgaacag
     2701 cagccccttc ttcaccgggt cttcgcgcac cacattcacc ggcgcattct caggcagccc
     2761 agcgacaatt tcctgccagc tttggccgaa atcgcgcgtg cgataaatgt gcggatgcaa
     2821 gtcatcgagc cggaaacgat tcaccgcggc gtacgccgct cccgcggaag tgcgcgacgc
     2881 ctcaatgata gaaaccttgc tccaaggcgt cagcgccgga ggcgtcacgt tggtccagct
     2941 cttgccgccg tcgcgcgtca actgaatcaa accatcgtcg gtgccggccc agatcgttcc
     3001 cttctccaaa tacgaaggcg ccagcgcgta aaccacgccc cgatgtttcc ctttttctgg
     3061 gtcgccagcc gcaaacgcgc cgatgttcgg cggattttcg tacgacggcc gcgcgaggtc
     3121 ggggctgatc accttccagt tctttccgcc gtcgctcgtt tcaaacacca cgttcgccgc
     3181 gaagtacaac ttgtgcggat cggtcggcga aaataccagc ggcagcgtgc ggtcataacg
     3241 aaattctccg cgccgtaccg gctccggcga aacatcctgc acttcgccca gcgccaagtt
     3301 ggtacgcgac acgcgcccgc cataaacaat gttgttgtcc aagggatcga cggcgatggt
     3361 tccgtattcc tcgacgccga gcaaccgcca gtcgcgaaac gtaatttcgc cgtagtcgct
     3421 gcggctgatc gtcgccgccg aaccgctctc ctgctgcgcg ccgtacaccc aataaggaaa
     3481 tctattgtcg gtggtgacgt gataaaactg cgcggtcggc tggttgtacc aactgctcca
     3541 ggtttcgccg ccattcaaac tgatgaccgc gccctgatcc gcagacagcg caatgatttt
     3601 tccatcagtc ggatggatcc aaatgcgctg gtagtcgtcg ccgcccggag cgcccttcca
     3661 tcccacgaaa gttttcccac cgtcggtgga tttccacgaa gcagtattca gcacgtaaag
     3721 cacatcagga ttgtctggcg ccacgccgat acccatggcc cctggacccc gcccgccaat
     3781 gcggtgatcg ccgttgacca gcgtccacga ttcgccccca tcgttcgaac ggtaaactcc
     3841 cgcgcctttt tcggcttcaa ccgatgcata gagacgattg gggttacttg gcgcaatggc
     3901 tacaccgatg cgccccagtt tttcttctcc agtcggcaac cccttggtca gcggcttcca
     3961 tgtcgaaccg cggtccgtgg atttgtaaag gccgcttcct gcgatataaa ttgacgcgcc
     4021 gctgcgcact tcccacggcg ccactcgtgc cgcccacaag gccgcataga gaatttgcgg
     4081 attcgccgga tccattacca gctcagccgc gccgatgttt tcatctttgt agagaacttt
     4141 ttcaaaggat ttgccgccgt ccgttgagcg gaacaccccg cgctcgacgt tcggtccgta
     4201 gggatgccct tgcgccgcca catacacacg ttccggatct ttagggtcaa ccacaatcgc
     4261 cgtgatctgc tgcgcgtccc gcaatcccag atgcgtccag gtttttccgg cgtcggcgga
     4321 tttgtagata ccgtcgccaa ccgccaagtc gggccgttgc aaaccttctc cgctgcccac
     4381 gtaaatcaca ttcggattcg acggagcgac agcaatggcc ccgactgatc cgctttctcc
     4441 ggcttcatcg aagatcggcg tccaggtgtt ccccgcgtcg ttcgacttcc acacgccgcc
     4501 attcaccgca gccatataaa acaaactcgg ttggcccgac acgcccgtaa tagccacagt
     4561 tcggcccgcg cgaaacggcc caatgcaacg ccaacgcatc tcttgaaaaa acttttgatc
     4621 gtacgtctgc gctgttgttg accgggccga tgcggcaatc gccaccactg ccacacacca
     4681 acgcatcgcc agagagaagt tcatcttggt gcctccaaag cgcattagga tagcgcgcac
     4741 ggtgatggcg ggcaatttga acatccaact gagttgtgtt ggctgaattt gcgcgatggt
     4801 tctgtcgaag cggcctatct ttttgcccga tccctaattt gcttcgcaag cttttcgatg
     4861 tcttgcgtgc gcttgaggac cgtcagactc agggtggcgt cgggatttgc gtggtccact
     4921 tcatccttca attccgtgga aagtgcgtag agacgttgca cgtccagacg caactcaagt
     4981 tggtcgttcg cctctcttgc cagtttttcg ctcttgtaga ctaccggcgg cccgttgagc
     5041 agcgcctgat tcgcggcaaa tccgggaagt ttaggcgcgc gggtctgccg cgccttcaga
     5101 aacaaaggtg taaaaaacca aatggccaaa acaaacgcgg cggcaaccaa cactcgcgag
     5161 gacgaacgct tcatgggtcc tccaccaagc ctgggatgaa gctgatggcc gaacgttata
     5221 cacctgtgta aagcaaaagc gaagagaaaa tccaaaagcc aagcaaatgg aagtggagtt
     5281 accaatgacc cagcgttcaa aaccgaagtt atttcttacg cgcgcttttc gcctttgcca
     5341 gacgacgcgg ccgcagcttt gcggacactg acttgatgat gaaccgcctc ttttgaccgg
     5401 ttggaattcc cactttggcg cgcttcaacg catgaccgat cagcgtgcgg acgtaggaat
     5461 cat
//
LOCUS       DASTPM010000524         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_566522, whole genome shotgun sequence.
ACCESSION   DASTPM010000524 DASTPM010000000
VERSION     DASTPM010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566522"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFR42_09270"
     CDS             complement(<1..752)
                     /locus_tag="VFR42_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0369387.1"
                     /translation="MPVLFLSLALPRHQGAMPFPWLFFAMTGVIFLGVLIVTIFFLRR
                     MMGGPASDKSIDKDPIPRAANEPAFMTASMQAVIQKLREQEKELERLHKIERERAEST
                     ARFSEEVTRNMPAGLLVIGANGLISSANPAAEQALGIKTLVFRRYSEALGEHTALSRL
                     IEQCLADGTVFRREQAAHIAPDGSHRELGVTISPIRRGQEKATGAICLLSDLTELATL
                     QKQMQLRENLAALGELSAGIAHEFKNALATISG"
     gene            complement(762..1661)
                     /locus_tag="VFR42_09275"
     CDS             complement(762..1661)
                     /locus_tag="VFR42_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU0369388.1"
                     /translation="MTIAAFFLFGLVIGSFLNVCITRIPEGASIISPRSRCPNCATPI
                     KPYDNVPVLSWIFLGGKCRNCHTSISPMYPVVEVLTALLFVACYLCFGLTLPALKWTF
                     FVCVLIVLVVTDFRERILPDVVNWFGVGLGLAFAAYVPPVDGVAPMLLRRLIALPRWT
                     SGVLDALLGAIFCGGLLWLTAWLYKVIRHRDGMGFGDVKMMFMVGTFLGLRGAFLTIL
                     IGTFLGTIVGLSVIGFLFVSGWKKSLAERASKMGLGGVAAIRWAIASRYQLPLGSFLG
                     IAALLVTFFSPWTLSRCAVLIFG"
     gene            1808..2833
                     /locus_tag="VFR42_09280"
     CDS             1808..2833
                     /locus_tag="VFR42_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369389.1"
                     /translation="MNWLEQRIRRYEHRRWTTDDNRRVRPFEWGLEHIGGRADEADPA
                     AYLLDYSRSAVENSRVWYEVAPADDYRLDSQNVLTFPSSLESPWPENNTVHAQLFPAK
                     SKKAAVLVLPNWNAKWHGQRGLCKWIQRTGITALKMSLPYHDRRMAKGHERADQLVGS
                     NIGLTLQANRQAVQDAKRCLRWLEQQGYSRIGILGTSIGSSVGYITLMHDAGVHAGGF
                     FHVSTYFADVVSQGMTTNHVWEGLREHVTVDELRQYWAPISPMPFVERGMGANRNSFM
                     VYGKYDPTFLPELTTEMLEALHEHGASPKALELACGHYSLELAPFSYIAGYRMLTYLR
                     SALAKTR"
     gene            2849..3307
                     /locus_tag="VFR42_09285"
     CDS             2849..3307
                     /locus_tag="VFR42_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369390.1"
                     /translation="MAPVVPLTTSNSIPSLDLKQNHAPLLDPAHWYVASDSRNQEKRV
                     LKEQILSDRMKHVRAGARVNSRVPISVEWEEAKARHSVNGWTVDVGPKGCLAIVPQGF
                     AIGQKLRLINLVNKNSVDAILIWRGHEGRTGWELGLELQDAGEEFWGVDF"
     gene            3486..>4185
                     /locus_tag="VFR42_09290"
     CDS             3486..>4185
                     /locus_tag="VFR42_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU0369391.1"
                     /translation="MTAIITIANQKGGVGKTTTAINLSAAIANRGKRTLLIDLDPQAN
                     STIAFFDGGEVAASMFDVLSDARAPMANVIKQTKDPMLAVGPGRLALAKLEQVLAGQF
                     DAPYRLKDALGPVLKDFDYLVLDTPPSLGILTVNALVCSTHLLVPIQAAYFAIEGTDD
                     LLETYERIRARPNPGLKMLGVVITLFDKRTNISRDTHGQIRTVFGEVLFKTKISKNVR
                     LEESPAYKETILTFA"
BASE COUNT          985 a         1096 c         1306 g          798 t
ORIGIN      
        1 ccggaaatgg tggccagggc gtttttgaat tcgtgggcaa taccggcgga gagttcgccg
       61 agggccgcaa gattttcgcg aagctgcatc tgcttctgca gcgtggccag ttcggtcagg
      121 tcgctaagca ggcagatcgc gcccgtggcc ttttcctgac cgcgccgaat cggggaaatc
      181 gtgacgccaa gttcgcgatg tgagccgtcc ggggcgatgt gagcggcttg ctcgcgccga
      241 aagactgtgc cgtcggcaag gcactgttca atgagcctgg aaagcgcggt gtgttcgccg
      301 agagcttcgc tgtagcgacg aaacaccagc gtcttgatac caagagcttg ttccgccgcg
      361 ggattggcgc tgctaatcaa cccgttggcg ccgatcacga gcaggccagc tggcatgttg
      421 cgggtgactt cttcgctgaa ccgcgcggtg gattcggctc gctcgcgttc gatcttgtgg
      481 agccgttcga gttccttttc ctgttcgcga agcttttgaa tcaccgcctg catggaggca
      541 gtcatgaacg ccggttcatt tgccgcgcgt gggatgggat ctttgtcgat ggacttgtcg
      601 gacgccggac cgcccatcat gcgtcgcaag aagaagatcg tcacgatgag gacgccaaga
      661 aagatcacgc cggtcatggc gaaaaacagc cagggaaagg gcatcgctcc ttgatggcgg
      721 ggaagcgcga ggctgagaaa gagaactggc attgccggaa cctacccgaa gatcaggacg
      781 gcgcagcggg agagcgtcca gggactgaaa aacgtcacga gtagcgcggc aattcccaga
      841 aaagagccta gaggaagttg gtagcggctg gcgatagccc agcgtattgc agcaacgcca
      901 cccagcccca ttttgctggc gcgctcggcg agcgatttct tccatcctga aacaaagaga
      961 aaaccgatca ccgacaggcc aacgatggtg ccgagaaaag tgccgattag aatggtgaga
     1021 aaggcgccac ggaggcccag aaacgtcccg accatgaaca tcatttttac gtcgccgaag
     1081 cccatgccgt cgcgatggcg gatcactttg taaagccatg cagttagcca taacagtccg
     1141 ccgcaaaaga ttgcgccaag cagagcgtcg agaacgccgg aagtccagcg cgggagtgcg
     1201 atgaggcgac gcaacaacat gggagcgacg ccatctactg gaggaacgta agcggcgaat
     1261 gcgaggccaa gtccaacgcc gaaccagttg accacgtcgg gcagtatgcg ttcgcgaaaa
     1321 tcggtgacca ccaggacaat cagtacgcaa acaaagaagg tccatttcag ggctggaagc
     1381 gtgaggccaa agcacaggta gcaagcgacg aagaggagtg cggtgagcac ttctacgact
     1441 gggtacatcg gagaaattga ggtgtggcag ttgcggcatt taccgccgag gaaaatccag
     1501 ctgagcaccg ggacattgtc gtaaggcttg atcggagtgg cgcaattcgg gcagcgggag
     1561 cgcggggaaa tgatcgaggc gccttctgga atccgcgtaa tgcagacgtt caggaagctg
     1621 ccgataacaa gtccaaacag gaaaaaggcc gctatggtca cactttgact gtggcacagg
     1681 cggccgagag gctcaattac ggtgaagtac aggggtgtcg gatggctaaa ctccaagtac
     1741 ggcggcgggc ggaaaaactc ggttcaaaag gcggaaaggc gagtcgggta tagtagaaga
     1801 agggcttatg aactggctgg aacagcggat tcgcaggtat gagcaccggc ggtggactac
     1861 cgacgataac cggcgcgtac gaccgttcga atggggcttg gagcatattg ggggccgcgc
     1921 ggatgaagcg gaccctgcgg cttacttact ggattactcg cgtagcgcgg tcgagaacag
     1981 ccgggtatgg tacgaagtcg caccagcaga tgactatcgc ctcgattccc agaatgttct
     2041 gaccttcccc tcctcactgg agtctccctg gcccgaaaac aacactgtgc acgcgcaact
     2101 gtttcccgcc aaatccaaga aagcagcggt gttagtgcta ccaaactgga acgccaaatg
     2161 gcatggacag cgtggactct gcaaatggat tcagcgtacc gggattacgg cgctgaaaat
     2221 gagcttgccg taccacgacc ggcgcatggc caaggggcac gagcgtgccg atcagttggt
     2281 gggttcgaac atcggattga cactgcaggc gaaccggcaa gcggtgcagg acgcgaagcg
     2341 ctgcctacgt tggctcgagc agcagggata ttcgcgaatc ggaattttgg gaaccagcat
     2401 tggttcgtcg gttggataca ttacgttgat gcacgacgcg ggcgtgcatg cgggcggctt
     2461 ttttcacgtt tccacctatt tcgcggacgt ggtaagccag ggtatgacca cgaaccacgt
     2521 gtgggaaggc ttgcgcgagc acgtgaccgt agacgaattg cggcaatatt gggcgccgat
     2581 tagcccgatg ccgtttgtgg agcgcggcat gggggcgaac cgcaattcgt tcatggtgta
     2641 cggcaagtac gacccgacgt ttctgccgga gctgacaacg gagatgctgg aagcgctgca
     2701 cgagcatgga gcatcgccaa aggcgctgga actggcctgc gggcattatt cactggaact
     2761 ggcgcccttt agctacatcg ccggataccg gatgctgacc tatctacgct ccgcgttggc
     2821 taaaactcgc tagtaactga atcaggaaat ggccccggta gttccattga ccacgtccaa
     2881 ctctattcct agtctggacc tcaagcaaaa ccatgcgccc cttctcgatc cggcgcactg
     2941 gtatgtggcg agtgactcac ggaatcagga gaagcgcgtg ctgaaagaac aaatcctgtc
     3001 ggacagaatg aaacatgtgc gcgccggagc ccgcgtcaat tcacgcgtgc cgatatccgt
     3061 cgaatgggaa gaggccaaag cgcggcattc ggtgaacggc tggactgtag atgtcgggcc
     3121 gaaagggtgt ctggcgattg tgccgcaagg ttttgcgatt ggacagaaac tgcggctgat
     3181 caatctggtc aacaagaatt ccgtggacgc gatcttgatc tggcgtgggc acgaaggccg
     3241 caccggctgg gagcttggcc tggagcttca ggacgccggg gaagaatttt ggggagtgga
     3301 tttttagtcc gccgaagttt tccactgact accgcctaaa accgcacttt ttccttgcgc
     3361 ttcaatcgct taggaacgta gagttaccct aaggtctggg ggactattac gagcaacacc
     3421 tacatctggg aagccggttg cgggttcgcg ggattcgcga acagcgcgaa cggaggcgca
     3481 ttggaatgac cgccatcatt acgattgcca atcagaaggg cggagtcgga aaaactacta
     3541 cggcgatcaa tctttcggca gcaatagcaa accgcggcaa acgcaccctc ctcatcgatc
     3601 tggatccaca ggcaaactca acgatcgcgt tttttgacgg cggtgaagtc gcggcgtcca
     3661 tgttcgacgt gttgagcgat gcgcgcgcgc cgatggccaa cgtcatcaag cagacgaaag
     3721 atccgatgct tgcggtggga ccgggtcggc ttgcactagc taaattggaa caagtgctgg
     3781 caggtcagtt tgacgcgcca taccgactga aagacgcgct aggcccggtg ctcaaggatt
     3841 tcgactactt ggtgctggac acgccgccct cgctggggat cctgacggtc aatgcactag
     3901 tgtgttccac ccatctgctg gtgccgattc aggcagcata ttttgccatt gaaggcacgg
     3961 acgatctgct ggagacctac gagcgaatcc gcgcgcggcc gaacccgggc ctcaaaatgc
     4021 tgggtgtggt tatcaccctc ttcgacaagc gcacaaatat ttcgcgcgat acgcacgggc
     4081 aaattcgcac cgtatttggc gaggtgttgt tcaaaacgaa gatcagcaag aacgtgcggc
     4141 ttgaagaaag cccggcttac aaagaaacaa ttctgacgtt cgcgc
//
LOCUS       DASTPM010000525         6814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_941003, whole genome shotgun sequence.
ACCESSION   DASTPM010000525 DASTPM010000000
VERSION     DASTPM010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941003"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFR42_09295"
     CDS             complement(<1..372)
                     /locus_tag="VFR42_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369392.1"
                     /translation="MKPSLAVMCVLLLAAGGWLNSCAAPAENSIAITHVTVIDMTGAP
                     PLADQTVLVEEQRIAAVGATASIPIPRGMKIVDASGKFLIPGMADMHVHLTAAGEPDG
                     SRRFMIPLLLANGITTVRDMGG"
     gene            complement(402..1586)
                     /locus_tag="VFR42_09300"
     CDS             complement(402..1586)
                     /locus_tag="VFR42_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU0369393.1"
                     /translation="MHYRSVLFLIALIVLLPFALSAQNTAKKERARDLGVPFDGKPGK
                     FNAITDVPGVLVGHTTLISGEGKLQVGKGPVRTGVTAVLPRGKDSMNDAVFAGWFSQN
                     GNGEMTGTTWVEESGFLEGPVMITNTHSVGVVRDALIQWRVAHGKPDPTGYWWSLPVV
                     AETWDGWLNDINGFHVKPEHAFHAIDSAHGGEVEEGSVGGGTGMVCNGFKGGMGTASR
                     KLETKDGGYTVGVLVQCNYGSRQNLRVAGIPVGREISSEDPYAYQPSEISERGSIIVV
                     VATDAPILSQQLKRLARRVSLGLGRNGSISGDGSGDIFIAFSTANPHAEGGKSVVDLK
                     FLPNDALDPIFAATVQAVEESIINAMIAAPTMTGIENHKVTGLPHNKLRDVLKKYNRL
                     VQ"
     gene            complement(1654..2991)
                     /locus_tag="VFR42_09305"
     CDS             complement(1654..2991)
                     /locus_tag="VFR42_09305"
                     /inference="COORDINATES: protein motif:HMM:NF005685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0369394.1"
                     /translation="MSNVRSLFPRHFQKEFPAAVRGEGCWIIASDGRRYLDASGQAAV
                     VNIGHGVKEIGAAMAAQAGQVAFAHTTQFHSEAAEKLAARLLALAPANFRNGGRVYFT
                     SGGSEATETAIKLARQFHLESGQAARYRVISRRQSYHGSTLGAMSVSGNVGRRAPYAP
                     LLAKWGHVAPCFCYHCPFDKRFPECGIACAEDLANLLRANDPASAAAFIFEPVVGATL
                     GAAAPPDGYVQRIAEICREQGILLIADEIMTGMGRTGRVFAVDHWNVEPDLILVGKGV
                     ASGYAPLGGVLVSARVAEVFAKGSGAFQHGFTYQAHPVATAAGNAVLDFLEQHQLFER
                     VGPAARQLRGALKPLEAHPQVGEVRGMGLLLGIEFVKDKKTREPFAKELNIAEKIRQA
                     ALQKNALTYPSQGCVDGVRGDHILLAPPFIISGEECSQIAQALQYAVAQTLAV"
     gene            3086..4993
                     /locus_tag="VFR42_09310"
     CDS             3086..4993
                     /locus_tag="VFR42_09310"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369395.1"
                     /translation="MKRIAIVCGLFVSIAVFSAATVTAQSQPPVKLEVDLRDAPRRIY
                     HSKMEFPVKPGPFTLVYPKWIPGEHSPNGPIVDVTGVHFSANGQEISWRRDDVDLYAF
                     HCNIPAGVTALEATLDYLLPKEDGTSGSPSATAQVAVLPWNLVVLYPEGAKTDDVMLS
                     ASVRVPAGWKFATALTTERSEGAEAAAFETVSLTRLVDSPLFAGAFFKTYDLSPGQKP
                     EHRLNLVADSAIATTVTAEQLTHFRQLIAETGALFGARHYRHYDFMLALTDHLPVNGL
                     EHHESSDNRGPEKMLLDPDALEGLMDLLPHEFFHSWNGKYRRPSGLATPNYQQPMKGG
                     LLWVYEGLTQYYGVMLAARSGFWKPEQLREYLAATFAALNDGRPGRTWRNLEDTAISV
                     SILYGARQAGSSWRRPVDYYEESELIWLEADTIIRRETKGQKSLDDFCRRFHGTEDGV
                     IKVAPYTFDDVIATMNSVAPYDWRQFFEDRIHSHGPGAPLGGLENSGWKLTYSDDMNE
                     HQRAAEQADGIVDLSFSLGIIAQAAGDDANSMVDVIPGSPAAKAGIAPGMKLVAVNGR
                     KWTPDWLRVAIRDAKNSKDPIRLLLENDEYYHTYEIDYHGGERYPHLESLHTTDVLSE
                     IAKHRAPAVVK"
     gene            4995..6293
                     /locus_tag="VFR42_09315"
     CDS             4995..6293
                     /locus_tag="VFR42_09315"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0369396.1"
                     /translation="MTTQTPELVRTIGRWSLVALMANSILGAGIFGFPALLAARLGGY
                     SPLSCIVAGCGALIIAACIAEISSRFETTGGLYLYGREALGRFPGLLIAWLILLTRIA
                     APAAAADLFANYLGQFFPSLHGKLPEICLIGALIGHLALLNYLGVKTSKTVSDIFTTV
                     KVGLLTVFIVAGLAIPMLHPQLRVPLHLGATTPQGWFEALLLLVFGYGGFEGALIVGG
                     ESRNPKRDMPFALLTALVLQVFLYTGVVYVVLSTLPNAGASLRPLADAARSFLGGWGA
                     TAIGLGALISTYGYLSANLLHSTRITFSLAEQGDFPEFFARIHARFRTPHISILVYSL
                     AVFAFAASGDFRWNAILSAATRLFVYGGMAIALLMLRKKSGPAPFSLPFPWLFAGAAL
                     LIVLVLLSRIGSGEAVVLAMTAAMALANWFVLKRAAIRTN"
     gene            6476..>6814
                     /locus_tag="VFR42_09320"
     CDS             6476..>6814
                     /locus_tag="VFR42_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369397.1"
                     /translation="MSVAKRSLLCVVYAAGLLFASGASLRAQEKHAITFDDLISMHRV
                     AEAEISPDGQWVAYTVATPDMDANRNATNLWLAPVAGGDAIQLTRTGKDSSPKWSPDS
                     KTIAFLSARSG"
BASE COUNT         1373 a         2307 c         1721 g         1413 t
ORIGIN      
        1 gccgcccatg tcccgcaccg ttgtaatccc attcgccagc agcagcggaa tcatgaacct
       61 ccggctgccg tccggctcgc ccgccgccgt caggtgcacg tgcatatccg ccatcccggg
      121 aatcagaaat ttcccggacg catccacaat cttcatgcca cgaggtatgg gtatagaagc
      181 cgtcgccccc actgcggcga tgcgttgttc ttctaccagg acggtttggt ccgcgagcgg
      241 gggcgcgccg gtcatatcga tgaccgtgac gtgcgtaata gctatcgaat tttccgccgg
      301 cgcggcgcag gaattcagcc agccacccgc ggccaacagc aacacacaca tcaccgcgag
      361 cgatggtttc aagtaggccc tttttcagca aacaaaaatc tctattgcac cagacggttg
      421 tattttttca gaacatcccg caatttgtta tgcggaagac ccgttacttt atggttttcg
      481 atgccggtca tcgtcggcgc ggcaatcatc gcattgatga tggactcttc caccgcctgc
      541 accgtcgccg caaaaatcgg gtccagcgca tcgttcggca aaaatttcaa gtccaccacg
      601 gactttccgc cctccgcgtg cggattcgcc gttgaaaacg cgataaagat atcacccgat
      661 ccgtcgcccg aaatgcttcc gttgcgcccc agccccagcg aaacgcgccg cgccagccgc
      721 ttcaactgct gcgaaaggat gggcgcatcc gttgccacca ccacgatgat cgacccgcgc
      781 tccgaaattt ccgaaggctg ataggcgtac ggatcctccg aagaaatctc ccgccccacc
      841 gggattcccg caacgcgcag attctgccgc gagccataat tgcattgcac cagcacgccc
      901 accgtgtacc cgccgtcctt cgtttccaat ttccgcgacg cggttcccat gccgcccttg
      961 aacccgttgc aaaccattcc cgttccgccg cccaccgatc cttcctctac ctctccgcca
     1021 tgcgccgagt caatcgcgtg aaacgcgtgt tccggcttca catgaaaccc attgatgtcg
     1081 ttcagccagc catcccaggt ttccgccacc accggcaatg accaccagta gcccgttggg
     1141 tccggctttc catgcgccac gcgccattga atcaacgcat cgcgcaccac tcccacgctg
     1201 tgcgtgttcg tgatcatcac cggcccctcc agaaaccccg attcctccac ccacgttgtg
     1261 ccggtcattt ctccgttgcc gttctgtgaa aaccatcctg cgaacaccgc atcgttcatc
     1321 gaatcctttc cccgcggcaa aacggcggtt acccccgttc gcaccggccc ttttcccact
     1381 tgcagtttcc cttcgccaga aatcaacgtc gtatgtccca ccagcactcc tgggacatct
     1441 gtgatcgcat tgaattttcc tggcttgccg tcaaacggaa cgcccagatc acgagcgcgc
     1501 tccttcttgg ccgtgttttg tgcggacaaa gcaaatggca acaggacaat caaggcgatc
     1561 aaaaacagaa cagaacgata gtgcatgagg gatttctctc ccaacgaaat atgagtgcaa
     1621 ccaccggtat gaatcgaacg actgtagcgt aactcagacc gccaatgttt gcgccactgc
     1681 gtattgcagc gcttgcgcga tttgtgaaca ctcttccccg gaaataatga acggcggagc
     1741 cagcaggatg tgatcgccgc gcaccccgtc gacgcatccc tgtgacggat acgtcagcgc
     1801 atttttctgg agggcagcct ggcggatctt ttccgcaatg ttcaattcct tcgcgaatgg
     1861 ctcacgggtc tttttgtctt tcacaaactc gatgcccagc agcaacccca ttccgcgaac
     1921 ctcgcccacc tgcggatgcg cttcgagcgg cttcagcgcc ccccgcagtt gccgcgctgc
     1981 cggaccaaca cgttcgaaca actggtgctg ctccagaaaa tccagcaccg cattccccgc
     2041 cgccgtcgcc accggatgcg cctgatacgt aaacccgtgc tgaaacgctc ccgaaccctt
     2101 cgcaaagact tccgccacgc gcgccgagac cagtacccct cccagcggcg cgtacccact
     2161 tgctactccc ttgcccacca gaatcaaatc cggctcaaca ttccaatgat ctaccgcgaa
     2221 aactcttcca gtgcgcccca tgccggtcat gatttcgtcg gcaatcagca aaatcccctg
     2281 ctcgcggcaa atctcagcga tccgctgcac ataaccgtcc ggcggagccg ccgcccccaa
     2341 cgtagcgccc accaccggct caaaaataaa cgctgctgcg ctcgcaggat cattcgccct
     2401 caacaaattc gccaagtctt ccgcacacgc gatgccgcac tccggaaatc tcttgtcaaa
     2461 cgggcagtgg tagcaaaaac aaggcgcaac gtgtccccac tttgccaaaa gcggcgcgta
     2521 cggcgccctt cggccgacgt tcccgctcac cgacatcgct cccagcgtgc tcccgtgata
     2581 gctctgccgc cgtgaaatca cccggtaacg cgcagcctgc cctgactcca gatgaaattg
     2641 tcgcgccagt ttgatcgccg tttccgtagc ttccgatccg cccgaagtga aatagacccg
     2701 cccgccattc cgaaaattgg cgggagcaag cgcgagcagc cgcgccgcta gtttttccgc
     2761 tgcttccgaa tgaaactgcg tcgtgtgcgc aaacgcaacc tgccccgctt gcgccgccat
     2821 cgccgcacca atctctttca ccccatgccc gatattgacc acagccgctt gcccggacgc
     2881 gtcaagatac cgccgcccat cggaggcaat aatccagcac ccctcgccac ggacggcagc
     2941 aggaaattct ttctggaaat gacgcggaaa gagcgacctg acgttggaca taatccctgg
     3001 ccaaagttta ccagaccgat gtcgaggctg ccttcaggcg gcctccgcct gtgatacgct
     3061 ggcccgtaat tttttgggag cattcatgaa gagaattgca atcgtttgcg gtttgttcgt
     3121 gtccattgcc gttttttctg ctgcaaccgt aacagcccaa tcccaacctc ccgtaaagct
     3181 agaagtcgac ctccgcgacg cccctcgccg catctaccac tccaaaatgg aattccccgt
     3241 taagcccggc ccgttcaccc tcgtctaccc gaaatggatt cccggcgaac actctcccaa
     3301 cggcccgatc gtcgacgtca ccggtgtcca cttcagcgcc aacggccagg aaatttcctg
     3361 gcggcgcgac gacgtcgatc tctacgcctt tcactgcaat atccccgcgg gcgtaaccgc
     3421 tctcgaagcg accctcgact acttactccc caaagaagac ggcacttctg gctcgccttc
     3481 cgccaccgct caagtcgccg ttctcccgtg gaaccttgtc gtgctctacc cggaaggcgc
     3541 gaagaccgac gacgtgatgc tttccgccag tgttcgggtg cctgccggat ggaaatttgc
     3601 cactgcgctc actaccgaac gcagcgaggg cgcggaagca gccgccttcg aaaccgtctc
     3661 cctcactagg ctcgtggatt cccccttgtt cgcaggagca tttttcaaaa cctacgatct
     3721 ttcgccagga caaaaaccgg aacaccgcct gaaccttgtc gccgacagcg ccatcgccac
     3781 caccgtgacc gccgaacaac tcacgcattt tcgccagctc atagcggaaa cgggagccct
     3841 cttcggcgcg cgccattatc ggcactacga tttcatgctg gcccttaccg atcacctgcc
     3901 cgtcaacggc ctcgagcacc acgaatccag cgacaatcgc ggcccagaga aaatgcttct
     3961 cgaccccgac gctctcgaag gcctgatgga tttgctcccg cacgaatttt ttcattcctg
     4021 gaacggcaaa taccgccgcc cctccggtct cgccacgccc aattatcagc aaccgatgaa
     4081 gggcggtctg ctctgggtct atgaaggcct cacgcagtat tacggcgtca tgctcgccgc
     4141 ccgcagcggc ttctggaagc ccgagcaact ccgcgaatat cttgcggcca catttgctgc
     4201 tctcaacgac ggtcgccccg gccgcacctg gcgcaacctc gaagacaccg ccatttccgt
     4261 ctcgattctc tacggcgcgc ggcaagctgg atcctcctgg cgccgtcccg tggactacta
     4321 tgaagaaagc gagctcatct ggctcgaagc cgacaccatc attcgccggg aaaccaaagg
     4381 ccagaaatcg ctcgacgact tttgccgccg cttccacgga accgaagacg gcgtcattaa
     4441 agtcgctccg tacacttttg acgatgtcat cgccacgatg aattccgtcg cgccctacga
     4501 ctggagacaa ttcttcgaag atcgtatcca ctctcatggt ccgggcgcgc cgctaggcgg
     4561 tctggaaaac agcggctgga agctgaccta cagcgacgac atgaacgaac accagcgcgc
     4621 cgcggaacag gccgacggca tcgtcgacct ctcgttctcg ctaggaatca tcgcgcaagc
     4681 cgccggcgac gacgcaaatt ccatggtcga cgtcattccc ggatcgccgg ccgccaaggc
     4741 tggcatagcg ccaggaatga aactagtcgc ggtgaacggc cgcaagtgga cccccgactg
     4801 gctccgcgtc gccatccgcg acgccaaaaa ttccaaagac ccgattcgct tgctgctcga
     4861 aaacgacgag tattaccata cgtacgaaat cgactatcac ggcggcgaac gctacccgca
     4921 cctcgaatcc ctgcacacca ccgacgtgtt gagcgaaatc gccaaacatc gcgctccggc
     4981 ggtcgtaaag tgatgtgaca acgcaaacgc cggaactcgt gcgcaccatc gggcgatgga
     5041 gtcttgtcgc cctcatggcc aactccatcc tcggcgccgg aatttttgga ttccctgctc
     5101 tcctcgccgc gcgcctgggc ggctacagcc cgctgagctg tatagttgcc ggctgtggcg
     5161 cactcatcat cgccgcatgc atagcggaga tttcttcgcg ctttgaaacc accggcggtc
     5221 tctatctcta tggccgcgaa gccctgggtc gttttcccgg cttgctcatt gcgtggctga
     5281 ttctcttgac tcgcatcgcc gcgcccgctg ccgcggccga tctttttgca aactacctcg
     5341 gacaattctt cccgtcgctg cacggaaagc tcccggaaat ttgtctgatc ggcgcgctta
     5401 ttggccatct cgcgctgctg aattacctcg gagtgaaaac cagcaaaacc gtcagcgata
     5461 ttttcactac cgtaaaagtc ggactgctca ccgttttcat tgtggctgga cttgcgattc
     5521 cgatgcttca cccgcagctg cgggtcccac ttcatcttgg cgccacaaca ccacaaggct
     5581 ggttcgaggc gctgttgttg ctggtttttg gatacggcgg cttcgaaggc gcgctcattg
     5641 tcggcggcga atcgcgcaat cccaaacgcg atatgccttt cgcgctgctc accgccctgg
     5701 tcttgcaagt ttttctctac accggcgtcg tctacgtggt attgagcacg cttccaaacg
     5761 cgggcgcttc acttcgcccg ctcgccgacg cagcgcgcag ttttctcggc ggttggggag
     5821 cgaccgcgat cggcctgggc gcgctcattt ccacttacgg gtatctcagc gcgaatttgc
     5881 tgcattcaac gcgcattact ttttccctcg ccgaacaagg agactttccc gaatttttcg
     5941 cgcgcattca tgcgcgattc cgcactccgc atatttcaat tctggtctac tcgctcgccg
     6001 ttttcgcatt cgcggccagc ggcgattttc gctggaatgc cattctttct gcggccaccc
     6061 gcctcttcgt gtacggtgga atggcaatcg cgctgctcat gttgagaaaa aagagcggcc
     6121 ccgctccgtt cagcctgccg tttccctggc tgttcgccgg cgccgcgctg ctgatcgtct
     6181 tggttctgct cagccgtatc ggatccggtg aagcggtcgt attggccatg actgccgcca
     6241 tggcgctggc caactggttc gtactgaagc gtgctgcaat caggacgaac tgaccgaagg
     6301 gttcggccaa actggcgcca gtttcgcgcc tcgctggaag ctccgcggtt gctgtttggg
     6361 gcatcgcttc atgtcggcat cgcttcattg tcaatggacg aggaaaaggc ataagatttt
     6421 gcgtttgcag aaatgtgctg agcgtcggcg gttttctcaa ggagagagca tgagaatgag
     6481 tgtggccaag cggagcctgc tgtgcgtcgt ttacgcggcg gggttgcttt tcgcgagcgg
     6541 cgcaagtctc cgcgcgcaag aaaaacacgc gatcacgttt gacgatctga tctccatgca
     6601 ccgcgtcgcg gaagcggaaa tttctccgga tggacagtgg gtggcgtaca ccgtggcgac
     6661 tccggacatg gacgcgaacc gcaacgccac gaatttgtgg ctggcgccgg tcgcgggcgg
     6721 cgatgccatc cagctcacgc gcactggaaa agattcttcg ccgaaatggt cgcccgacag
     6781 caagaccatt gcgtttctct cggcgcgcag cggc
//
LOCUS       DASTPM010000526         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_75785, whole genome shotgun sequence.
ACCESSION   DASTPM010000526 DASTPM010000000
VERSION     DASTPM010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75785"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..643)
                     /locus_tag="VFR42_09325"
     CDS             complement(281..643)
                     /locus_tag="VFR42_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369398.1"
                     /translation="MRPITNVGGCSYEAAKETSGGWRRNVAVNVSKYKTASDEASAWD
                     EQKILRSLRPGRKNLTVLSGIGSEAYLQISPDRKSFDGEVWVHKTLSHFRLIEVSEQP
                     PSPDVLKAAAQKIAGKLP"
     gene            1076..>2591
                     /locus_tag="VFR42_09330"
     CDS             1076..>2591
                     /locus_tag="VFR42_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369399.1"
                     /translation="MKKSEAAKYARWSAAAALVCAGLTVGVYLKRGWTRYNERKSAPP
                     SPAVNVERQSTKLTFSKGEGARTVFTVEAMKSTDFRGLNATDLEGVKVTIFGKDGARH
                     DMLETHACRYSKDSGDIDCSGDVEIRLMSKLEWEAAQEQSAIGAGAAMKVETRGVVFN
                     RASGEARTDQAVRFTFANGGGQAVGATYHSEEGTLTLLHNVRLKLDPPASTKAKVAPG
                     GLIAAAKEPVEVTGSRMEFQRDAGTMYLAGPAEAKTKTERLTAAALQLSLDENFHAKR
                     LIAKGNGREFLPEFTSEKGAGRQRLQAEEIAADFAPAGWITRGEANGRVSGEAVKGDE
                     IQTVKSQSAEMEMVAGLNAPKLLVLQGGVDAATNAKAGGSKTESGDSRRLTTDELRIA
                     FAEKVGASGARLQSAETGGPGRLELNDAANPASKAARTVLQANQLNMKFDSMGAASRL
                     DAKGSVQTERTVAGGNKQTASANNGFVLMEPTGGWSRMELHENVQLSELQRAARA"
BASE COUNT          611 a          671 c          879 g          430 t
ORIGIN      
        1 cctggcggat gaggccatgt acctggtgaa gaacacgaac cgcgacagcg tagccgtagc
       61 taatgtgggg atcctgccgc acctgccggc ggctaacgcc gagaccgcag gctgacgcgg
      121 cgcaggcacc gcccgttacc cctccttgtt gtaagtgttc attccaaagc acgtacgaag
      181 gctagttttc ccaagcgggt cagatatcaa ccccgagcga cgccagggga aacgccgtgt
      241 ctcattgttt tttcgccaac cagtcgcccg gtcaccagga tcaggggagt ttgccggcga
      301 ttttttgcgc cgcggctttc agaacgtccg gagaaggggg ctgctcgctg acctcgataa
      361 ggcggaaatg ggagagagtc ttgtggaccc aaacctcacc gtcgaaggat ttccggtcgg
      421 gcgaaatttg caggtaggct tccgagccga tgccgctgag aacggtgaga ttttttcggc
      481 ctggcctgag gctgcggaga atcttctgct cgtcccaggc ggaagcttcg tcggacgcgg
      541 tcttgtattt cgagacgttc actgcgacat tgcggcgcca gccgccggag gtttccttgg
      601 cggcttcgta ggagcaacca ccgacattgg tgatgggacg cacgggaacg ccgagaactt
      661 gcgcggcgtc agcggcgcta aagacagtgc aagcatcgaa gcttatgggc tgtgggtcgc
      721 cctgtgacgt gtttggtgta gccgccggag tcgccgaact tggcggggca aaactctcgc
      781 cgcaggcaaa ggataggacc agcaatgagc aagcgaggac tggccctgaa acgatgcggc
      841 gtggactgcg ccaaatacga acgtacctgt cgaacctgcc agagtggaaa gacatggttg
      901 catcctttgg agttggtttt gcgatcggca gcgggcaaga aacgctgcgg cgagggcata
      961 gtaacgggag tggtcttaaa cgaaaagcaa aaggggatca agcgtgcgca ttctatacag
     1021 cgcgcgacta ttctgattaa ctgaacaaga tgccgccgta acaggtggca ctacgatgaa
     1081 aaagagcgaa gcggcgaagt acgcgcgatg gagtgcggcc gcggcgttgg tatgcgccgg
     1141 gctgaccgtg ggcgtgtacc tgaaacgtgg atggacgcgc tacaacgaga gaaaaagtgc
     1201 gccgccatcg ccggcggtga acgtggagcg gcagtccacg aaattgacat tttccaaagg
     1261 cgaaggagcg cgaacggtat ttacggtgga agcgatgaag tcgaccgact tcagggggct
     1321 gaatgccacc gatctggaag gcgtgaaggt caccattttt ggcaaagacg gcgcgcggca
     1381 cgacatgttg gagacgcacg cgtgcaggta cagcaaggac agcggcgata ttgattgctc
     1441 aggcgatgtg gagatcaggc tgatgagcaa actggagtgg gaagccgccc aggaacaatc
     1501 cgctattggt gcgggggcag cgatgaaggt ggaaacccgc ggtgtggtgt tcaaccgggc
     1561 cagcggcgaa gcaagaaccg accaagcggt ccgatttacg tttgctaacg gtggcggaca
     1621 agcagtggga gcgacctatc actcggaaga aggaacgctg acactgctgc acaatgtccg
     1681 gttgaagctg gatccaccgg cctccacgaa agcgaaagtc gcgccgggcg gtttgatcgc
     1741 cgccgcgaaa gaaccggtgg aagttaccgg ctcgcggatg gagtttcagc gggacgcggg
     1801 gactatgtac ctagccgggc cggcggaggc caaaaccaag acagaacgcc tgaccgccgc
     1861 cgcattgcag ttgagcctgg atgaaaattt tcacgcgaag cgcctgatag cgaaaggcaa
     1921 cggccgggaa tttctgccgg aatttacttc agaaaaaggc gcaggccgcc agcggcttca
     1981 ggcagaagaa attgcggcgg actttgctcc ggcagggtgg attacgcgcg gggaggcaaa
     2041 cggccgtgtc agcggagaag cggtcaaggg cgacgaaata caaacggtca aatcgcaaag
     2101 cgcagagatg gaaatggttg cgggtcttaa cgcacccaaa ctgctggtgt tgcagggcgg
     2161 cgtggacgcc gccacaaacg ccaaggcagg tggaagcaag accgaaagcg gcgactcgcg
     2221 cagattgacc accgacgagt tacggattgc cttcgcggaa aaggtgggcg cgagcggggc
     2281 gcgcttgcag agcgcggaaa caggcggacc cggccgcctg gaactgaacg acgcagcgaa
     2341 ccccgccagc aaggcggcgc gcaccgtatt gcaagcaaat cagctaaaca tgaagtttga
     2401 ttcaatggga gcggcttcgc ggctggatgc gaagggcagt gtgcagaccg agcgaaccgt
     2461 tgccggtggc aataaacaaa cagcgagcgc gaacaacggg tttgtcttga tggaaccgac
     2521 gggcggctgg tcgcgcatgg aactccacga aaacgtgcag ttaagcgagt tgcagcgcgc
     2581 cgcgcgcgcg g
//
LOCUS       DASTPM010000527         4542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_595192, whole genome shotgun sequence.
ACCESSION   DASTPM010000527 DASTPM010000000
VERSION     DASTPM010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595192"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VFR42_09335"
     CDS             complement(<1..1236)
                     /locus_tag="VFR42_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369400.1"
                     /translation="MTFMTLAQARENFAAAMQTLHSSKVRSALTVLGIVIGVSSVISM
                     AAIIQGLNKFVQDRVESLGSRTYFLTRFPPGTDPAHMPDKIRIRRYIGYDYAEFIRAA
                     APDVENINTVGTRGFFFGDSNRITSGDHTVEKVIVRGAEPEYTTALPLFNIEHGRFIS
                     AFDQEHARPVVVLGAAIAESLFPDSDPLGKTVRLNGSVYEVIGVFTHDQGLFLGPGVD
                     IFAIIPLSNFKKQYPEAKELILLFTVPHDVNIETAQAEVIQAMRRIRKIPADKENDFE
                     LSSPDFLSNLWNQLTGALVILTSVISSIGLLVGGVGVMNIMLISVTERTQEIGVRKAI
                     GARKADVRLQFLFEAVVLTLIGGTIGILIGAGVSATVRALAPSIPATLSYWWVSIGFV
                     MSVGVGLFFGYYPANLAANL"
     gene            complement(1236..2381)
                     /locus_tag="VFR42_09340"
     CDS             complement(1236..2381)
                     /locus_tag="VFR42_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369401.1"
                     /translation="MIGLTLGVATLITVMTIVQGANLYVENKIANLGTNVFQVARTPF
                     AVTDFTIIIKALKYKKIELDDMRAVAEGCLACQDVGATASATVRARYGDKEAQDVSFY
                     GQTPSMADIDTRAVEFGRYFSAAESEHRTNVCLIGDSLVQKLFVGVDPIGRSIRIGND
                     EFMVVGIMEKIGSVLGQDQDNFVTVPLSVFLRIQGLHSSLTINVKAREGSFEAAQDQA
                     QLVLRARRHLSGHMENDFFIGTKESYMALWRSISGAFFGVFILVSAISVVIGGIVIMN
                     VMLVSVTLRKREIGVRRAVGATQRDILNQFMSESLIQCLVGGILGIGLGFLVALLLRT
                     FSPFPASVRTWVAVMGVVMSSAVGLFFGIYPAVRASRLDPVVALRSD"
     gene            complement(2811..3233)
                     /gene="rplQ"
                     /locus_tag="VFR42_09345"
     CDS             complement(2811..3233)
                     /gene="rplQ"
                     /locus_tag="VFR42_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU0369402.1"
                     /translation="MRHLKSGSKLGRNPWHRRATLRNLVTNLFEHGRITTTLTRAKAA
                     RPVAEKLITLGKRDTLASRRQAAAYIFTPGVTKKLFSEIAPKFADRAGGYTRIISAGT
                     RVGDGAKVAILELVGYEFKKKEKTEKPKKEEPAEKAAS"
     gene            complement(3405..>4542)
                     /locus_tag="VFR42_09350"
     CDS             complement(3405..>4542)
                     /locus_tag="VFR42_09350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU0369403.1"
                     /translation="RFCRVMYRQALAWHAARRIYEVPEAASPASRRKELNMWKGFQKP
                     KRLAPELESLTEKYARFSAQPFERGWGTTVGNALRRALLSSIEGAAITAVKIEGVLHE
                     FSSIAGVVEDATDIILNLKQVPIKLNTDLPKTIYLNVEQPGPVTSAQIEEDADFAVLD
                     KSVYIATVSEGGKLQIEMRVKNGRGYVAADRNYDEDLPIGYIPVDSVHSPVRKVNYSV
                     EAARLGQMTDYEKLMIEVWTNGAITPNDAIGLAAKLVKDHMSIFINFEEPTDVIEAPA
                     EYSNDPRLEHLDRSVEELELSVRSYNCLKNANIQTIRELVQKSENEMLKTKNFGRKSL
                     NEIKEILVKMGLALGMKFDEHGRIIWPPLPSQTAAPAPEENLGL"
BASE COUNT          969 a         1184 c         1467 g          922 t
ORIGIN      
        1 gaggttggcg gcgagattgg cggggtaata gccgaaaaag aggccgacgc cgacggacat
       61 gacgaaaccg atcgaaaccc accagtagga gagggtggcc ggaatagaag gggccagggc
      121 gcggaccgtg gcggaaactc cagcgccaat cagaataccg atagtgccgc cgatcagagt
      181 cagaaccacc gcttcaaaca agaactgcaa acgcacatcg gctttgcggg cgccgatggc
      241 cttgcgtacg ccgatttcct gcgtgcgctc ggtgacggag atgagcatga tgttcatcac
      301 gccaacgccg ccaaccagca gcccaatgga gctgatgacc gaagtgagga taaccagggc
      361 gccggtaagc tggttccaga gattgctgag aaaatcgggc gagctcagct caaagtcatt
      421 ttccttgtcg gccggaatct tgcgaatgcg gcgcatggcc tggatgacct cggcttgcgc
      481 ggtctcgatg ttcacatcgt ggggaacggt gaagagcagg atcagttctt tggcttcggg
      541 atactgcttt ttgaaattgc tgagcggaat gatggcgaaa atgtccacgc cggggccgag
      601 aaacaatccc tgatcgtggg taaagacgcc gatgacttcg tataccgagc cgttgaggcg
      661 cacggttttg ccgagcgggt cgctgtcagg gaacagagac tcggcaatcg cagcgccaag
      721 caccaccacc ggacgcgcgt gttcctggtc aaaagcgcta atgaagcgcc cgtgctcgat
      781 gttgaagagt gggagcgccg tggtgtattc gggctcggcg ccgcgcacga tgaccttttc
      841 gacagtatgg tctccgctgg tgatgcggtt ggaatcgccg aagaagaagc cgcgggtgcc
      901 gacggtatta atgttttcca cgtcaggagc cgcggcgcga ataaattcag cgtagtcata
      961 tccgatgtag cgacggatgc gaattttgtc gggcatgtgc gcggggtcgg taccaggcgg
     1021 gaaacgggtc aagaaatagg tgcgagagcc gagcgattcc acgcggtcct gcacaaactt
     1081 gttcaggccc tgaatgatgg cggccatgga aatgaccgag gaaacgccaa tgacgattcc
     1141 cagcacggtc agggcggagc gcaccttgga cgagtgcagc gtctgcatag ccgcggcgaa
     1201 attttcacgg gcctgggcca gggtcatgaa cgtcatcagt ccgacctcaa ggcaaccacc
     1261 gggtcgaggc gcgaagcgcg gaccgccgga tagataccga aaaacaatcc gaccgccgaa
     1321 ctcatcacca cgcccataac ggcgacccaa gtgcggactg acgccgggaa cggcgaaaag
     1381 gtacgaagca gcagcgcaac gagaaatccc aagccaatac cgaggattcc gccaaccaga
     1441 cactgaatca ggctttcgga catgaattga ttgaggatgt cgcgctgggt agcgccgacc
     1501 gcccggcgca cgccgatttc ccgcttgcgc aaggtcacgc tgaccagcat aacgttcatg
     1561 atgacgatgc cgccgatcac aacggagatg gcgctaacca ggatgaatac gccgaaaaac
     1621 gccccgctga tactgcgcca caaagccatg tagctttcct tggtgccgat aaaaaaatca
     1681 ttttccatat ggccgctgag gtggcggcgc gcgcgcagga ccaactgcgc ttggtcttgt
     1741 gccgcctcaa agctgccttc gcgcgctttg acgttgatgg tcaggctgga gtgcaggcct
     1801 tggatgcgga gaaatacgga gagcggcacg gtaacgaagt tatcctgatc ctggccaaga
     1861 acactgccga ttttctccat gataccgacg accatgaact cgtcgttgcc aatgcggatg
     1921 cttcggccga tagggtcgac gccgacaaaa agtttctgta ctagggaatc accgatgagg
     1981 caaacgttgg tgcggtgctc ggattcggcg gcactgaaat agcggccgaa ctcgacggcg
     2041 cgggtgtcaa tatccgccat actgggggtc tggccgtaaa aactgacgtc ttgagcttcc
     2101 ttgtcgccat aacgagcacg cacggtggcg ctggcggtgg ctcccacgtc ctggcaagcg
     2161 agacaacctt cggcgaccgc acgcatatca tcgagctcga tttttttgta cttgagcgct
     2221 ttgatgatga tggtgaaatc ggtgaccgca aaaggagtgc gggcaacctg gaacacgttg
     2281 gtgccgagat tggcaatctt gttctccacg tagagattcg caccctgcac gatggtcatc
     2341 acggtgatga gtgtagccac acccagggtc aggccaatca tggtgagtaa agagcgcagt
     2401 ttatgggcgc gcatggcgct caggctctgc gcgagattgt cctggagggc aagcactagg
     2461 ctgagaacct ttttctaata ctgtaccgaa tccggcggga caaaggcagg caagtctcgc
     2521 gcagagcagg cctaggggat tacgaagaag ggaggcggaa gttacaaggt gggtcgacga
     2581 tttggcggca ggcaactctg gcgcgattcg cgaggcgacg caagtagcac ggataggaaa
     2641 acggtccgga cgcggaagca atctgcccac gcccggcaag taaaggcggg tggcgaaccg
     2701 gtcaaccgtc gctcgttttc agcaatccga ttccattgca acaaaagagc ccgccgaccg
     2761 ttcctcgttg cgggccgttt ttctgagaga aggcgcggaa gacttcgagg ctagctggcg
     2821 gctttttcgg cgggttcttc cttcttgggc ttttcggtct tttccttttt cttgaattcg
     2881 tagccgacga gttcgaggat ggcgactttg gcaccatcgc cgacgcgagt gccggcggag
     2941 atgatgcggg tgtagcctcc ggctcgatcg gcgaacttgg gggcgatttc gctgaagagt
     3001 ttcttggtga cgccgggggt gaagatgtag gcggcagctt ggcggcggga agcaagagtg
     3061 tcgcgcttgc cgagagtaat gagtttttcg gcgaccgggc gcgcggcctt ggcgcgagtc
     3121 aacgtggtgg tgatgcggcc gtgctcgaac aagttggtga cgaggttgcg caaggtggca
     3181 cgacggtgcc aggggttgcg gccgagtttg gagccggatt tcaggtgtcg catgattcag
     3241 tcttctccgt aaatcattaa aagcaattcg tcaggggcta aagcccgtcg tcggccgcgg
     3301 gaaatgccgg agctgaagtt ccgacctcca ggaaatccgc accctgaaaa ccacagggtg
     3361 cggagcccga catggaaggt tacccaaagg tggccgctag aatcctacag gccgaggttt
     3421 tcttcggggg ccggagcggc ggtctggctg ggtagcggcg gccagatgat gcggccgtgc
     3481 tcatcgaact tcatgcccag ggccaagccc atcttgacga ggatttcctt gatctcgttg
     3541 agagacttgc ggccaaagtt cttggtcttg agcatttcgt tttcgctctt ctgcaccagc
     3601 tcgcggatgg tctgaatgtt cgcgttcttg aggcagttgt aggagcggac ggagagctcg
     3661 agttcttcga cagagcgatc cagatgttcg aggcgcggat cgttggaata ttccgccgga
     3721 gcttcgatga catcggtcgg ctcttcgaaa ttgatgaaga tgctcatgtg gtccttgacg
     3781 agtttggcgg caaggccgat ggcatcgttg ggggtgatgg cgccgttggt ccacacttcg
     3841 atcatgagct tttcgtagtc ggtcatctgg ccgagacgag cggcttcgac ggagtagttg
     3901 accttgcgca cgggcgagtg gaccgagtcc accggaatgt agccgatggg caggtcttcg
     3961 tcatagttgc ggtcagcggc gacgtaaccg cggccgttct tgacgcgcat ttcgatctgc
     4021 agctttccgc cttcgctgac ggtagcgatg tagacggact tgtcgagcac ggcgaagtcg
     4081 gcgtcttcct cgatttgcgc ggaggtgacg ggaccgggct gttcgacgtt gagatagatg
     4141 gttttgggca ggtcggtgtt gagcttgatg ggaacctgct tcaggttgag gatgatgtcg
     4201 gtggcgtctt cgaccacgcc ggcgatcgaa gaaaattcgt gcagcacgcc ttcgatcttg
     4261 acggcagtga tggcggcgcc ttcgatggag ctgagaagcg cacgacgcaa cgcgttgcct
     4321 accgtggtgc cccagccgcg ttcaaatggc tgcgcggaaa aacgcgcgta cttttcggtc
     4381 agcgattcga gctcgggcgc gagacgcttg ggtttctgga aacctttcca catattcagt
     4441 tccttccttc tggaagccgg tgatgcggct tccggtactt cgtaaattct tcgtgctgcg
     4501 tgccaggcta aagcctggcg atacatgacc cggcaaaagc gg
//
LOCUS       DASTPM010000528         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1229404, whole genome shotgun sequence.
ACCESSION   DASTPM010000528 DASTPM010000000
VERSION     DASTPM010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229404"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..428)
                     /gene="rplS"
                     /locus_tag="VFR42_09355"
     CDS             complement(27..428)
                     /gene="rplS"
                     /locus_tag="VFR42_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU0369404.1"
                     /translation="MNPVIRKLHEVQLRKDLPEFRAGDSVRVNVRLLEGEGEKVKERI
                     QAFEGVVISKKGRASSATFTVRRVSFGTGVERIFPLHSPSISSIEVTGKGHVRRARLY
                     DLRDLRGKAARIKRAGKAREVAEAAATRAAQ"
     gene            complement(739..1521)
                     /gene="trmD"
                     /locus_tag="VFR42_09360"
     CDS             complement(739..1521)
                     /gene="trmD"
                     /locus_tag="VFR42_09360"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU0369405.1"
                     /translation="MRFDLITIFPEFFAGPLEHGIVRRAREARIIDVHIQDLREFTKD
                     RHRTVDDRPFGGGEGMVLKPEPLFEAVESLLGRSVGNAATQPTLEPNTAVVLLSAAGK
                     RFTQETAHRYAQLDRILFLCGRYEGVDERVAEHLATEEISIGDFVLSGGELPAALVVD
                     AVTRLLPGALGNEASSQNESFSLLERAEKSPTAALHKALSHGRTLLDFPHYTRPADYR
                     GWKVPEVLIGGNHAEVAKWRHRMALEKTLRNRPDLLETTEAQ"
     gene            complement(1618..2223)
                     /gene="rimM"
                     /locus_tag="VFR42_09365"
     CDS             complement(1618..2223)
                     /gene="rimM"
                     /locus_tag="VFR42_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU0369406.1"
                     /translation="MTSPADASAQKFVTVARILRARGNKGEVAAELLTDFPDRLHQQT
                     EVFLAANSAVPQKAALQSFWVDRNHTGQCVFHFAGVSSINDAEKLRGLNVQIPWDQRA
                     ELPSGTYFVTDLIGCSVFEPPSAAPHVVSSPCSLAESPAFLGQVRDVYFPGEGQPGTP
                     LLALDTPQGELLIPLAEDICRKIDLPARRIEVLLPDGLRDL"
     gene            complement(2236..2469)
                     /locus_tag="VFR42_09370"
     CDS             complement(2236..2469)
                     /locus_tag="VFR42_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU0369407.1"
                     /translation="MKELVETIARALVDHPEDVKVQTVDGPQATVVELRVHPEDLGKV
                     IGRQGRTAKAMRTLLGASGMKFHRRFTLEILEE"
     gene            complement(2753..2992)
                     /gene="rpsP"
                     /locus_tag="VFR42_09375"
     CDS             complement(2753..2992)
                     /gene="rpsP"
                     /locus_tag="VFR42_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU0369408.1"
                     /translation="MLTIRLSRIGKRKKPFYRVVVIESSRARNGRVVEAVGTYDPLKK
                     PAEIKLDAERVKYWLGSGAQPSDTVRSFIRLQKIA"
BASE COUNT          641 a          843 c         1077 g          640 t
ORIGIN      
        1 ggcgcggcga aagtgttggc gctggattac tgagcagcgc gggtagcggc ggcttcggct
       61 acttcgcggg ctttgccggc gcgcttgatg cgggcggctt tgccgcgcag atcgcggagg
      121 tcgtagaggc gtgcgcggcg gacatggccc ttgccggtga cttcaatgga ggagatggac
      181 ggcgaatgga gcgggaagat gcgctctacg ccggtgccaa agctgacgcg gcggaccgtg
      241 aaggtggcgc tggaagcgcg gcccttcttg gaaatgacca cgccttcaaa ggcctggatg
      301 cgctccttga ccttttcgcc ttcgccttcg aggaggcgga cgttgacgcg gaccgagtcg
      361 cccgcgcgaa actcaggcaa atctttgcgc agctgcactt catgaagttt gcgaatcacc
      421 ggattcattt tcgtattcca tgcagaccca ctcaggcggg gccagctctt ctaagacaat
      481 cccaaattat acgtgagaat gccccaattt actaacgatt tcgtatcttg cgatatttcg
      541 cgggctcttc gagcctgctc cagccaaacg tggccatgaa gatcgcaaac ccgccgccaa
      601 ccaggcagta ggggaggaac gcgggcccac cccagtaagt ttggtaaaaa aaccgacctg
      661 gtacgagcga cgcaagcgcc ggactgcata aggccacgcc catgacaaat agaccgacgc
      721 gtttcattgt tcgattcttc attgggcctc agtggtctcg agaaggtcgg ggcggttgcg
      781 gagtgttttt tcgagggcca tgcggtggcg ccatttggct acttcggcgt ggttgccgcc
      841 gatgaggact tcgggaactt tccagccgcg atagtcggcg gggcgggtgt agtggggaaa
      901 atcgagcagg gtgcggccgt gggagagcgc tttgtgcagg gcggcggtcg gcgatttttc
      961 agcgcgttcg agcaggctga aagattcatt ttgcgaagag gcttcgttgc cgagagcgcc
     1021 ggggagcagg cgagtgacag cgtcgacgac gagagcggcg gggagttcgc cgccggagag
     1081 aacgaaatcg ccgatcgaga tttcttcagt agcgagatgt tcagcgacgc gttcgtccac
     1141 accttcgtag cgaccgcaga ggaagagtat gcggtcgagt tgcgcgtagc ggtgcgcggt
     1201 ttcttgagtg aagcgcttgc cggcggcgga gagcagaact acggcggtgt tgggttcaag
     1261 tgtcggctga gtcgcggcat tgcctaccga acggccgaga agcgactcga cggcttcaaa
     1321 gagcggttca ggcttgagga ccatgccttc gccgccgccg aaggggcgat cgtcgacggt
     1381 gcgatggcgg tctttggtga attcgcgcag gtcctggatg tggacgtcga tgatacgggc
     1441 ttcgcgggcg cggcgcacga tgccatgttc gagggggccg gcgaagaatt cggggaagat
     1501 ggtgatcagg tcgaagcgca tcggagtttt aagtttagca ggtgcagggt gcggttgaga
     1561 tcctcccgcg ctgaagccct gcctgaggca ggcgggccgc ggcgctacgt ttaaacctta
     1621 gagatcgcgg aggccgtcgg gcaggaggac ttcgatgcgg cgggcgggga gatcgatctt
     1681 tctgcagatg tcttcggcca ggggaatgag gagttctcct tgtggagtat cgagagcgag
     1741 cagcggagtg ccgggttggc cttcgccggg gaagtagacg tcgcgtacct ggccgaggaa
     1801 tgccggagat tcggcgagag aacatggcga ggaaacgacg tgcggagcag cgctcggggg
     1861 ttcgaagacc gagcagccga tcaggtcggt gacaaagtag gtgccggagg gaagttctgc
     1921 gcgctggtcc cagggaattt ggacgttgag gccgcgcagc ttttcggcgt cgttgatgga
     1981 ggagacgccg gcgaagtgaa acacacactg gccggtgtga ttgcggtcca cccagaacga
     2041 ttgcaaggct gctttttgcg ggacggcgga atttgcggcg agaaaaactt ccgtttgctg
     2101 gtgaaggcgg tcgggaaaat cggtgaggag ttcggcggcg acttcgcctt tgttgccgcg
     2161 ggcgcggagg atgcgggcta cggtgacgaa tttctgcgcg gaagcgtcgg cgggagaagt
     2221 cattcggcgg cgggcttact cttcgagaat ttcaagggtg aagcgacgat gaaacttcat
     2281 gccggaggcg ccaagaagag tgcgcatggc tttggcggtg cggccctggc gaccgatcac
     2341 tttgccgagg tcttcgggat ggacgcgcag ttcgactacg gtcgcttgcg gaccgtcaac
     2401 agtctgtacc tttacgtctt ccgggtgatc cacgagtgcc cgagcaatgg tttcgacgag
     2461 ctctttcatc aggcacctcc tttactgacg cagggtttgt acgtacaaac cccacccctc
     2521 gtgtggtttc caggatcaaa cggattattt caggtcatct ctcaggtaac cagcccagaa
     2581 caatccgtcc agagtgcccg agcaggcaag ctacccgggc cgacgcgtgt cgacgacaac
     2641 ctccagctga aactctcccc taatcagatg caaattcaaa gatgcaaaac cccccgaatg
     2701 ccgcgggggg tctccaaaga tgcccaaact gcgatgcgtg agtggctaac ggctaggcga
     2761 ttttttgcag acggataaag ctgcgcacgg tatcgctcgg ctgagcgccc gatccgagcc
     2821 agtactttac gcgctcggca tccagcttga tttccgcggg cttcttaagc gggtcataag
     2881 tgcccacggc ttccaccacg cggccattgc gggcacgcga actttcgatc accaccacgc
     2941 gataaaaggg cttctttctc ttcccaatcc gagacagacg aatcgttagc acaaacacac
     3001 tcctcctgaa ccttcaattg tagcgtgtca ggtgggggca cggcaatgcc ggtctataaa
     3061 atgagcaact aagtagaaaa agcgttgcgc ggcagcaagg ccaggttatt cctgctggtc
     3121 cttgctgtgg tggagcgtgt tcttgatgtc aggccagaac tcgcggaaca cgttccaggc
     3181 ccatcctgcc gccagggccg t
//
LOCUS       DASTPM010000529         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_191638, whole genome shotgun sequence.
ACCESSION   DASTPM010000529 DASTPM010000000
VERSION     DASTPM010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191638"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..2005
                     /gene="ilvD"
                     /locus_tag="VFR42_09380"
     CDS             284..2005
                     /gene="ilvD"
                     /locus_tag="VFR42_09380"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0369409.1"
                     /translation="MDSRLREHSRIIVDGPSRAPARSYFRAVGLTDDDLAKPLVGVAN
                     TWIEAMPCNSHLRHLAEKVKEGIRAAGGTPMEFNTVAISDGESMGTEGMKASLVSREV
                     IADSIELVGRGYMFDAMVALAGCDKTIPATAMGLIRLNLPSLVLYGGSIQPGRFRGRD
                     VTIQDVFEAVGAHSAGKITDADLKEIESVACPGAGACGGQFTANTMAMALEFIGLTPM
                     GLGNVPATDPRKDDAAYRSGTLVMELLRKGITPRQILTRKAFENAITAVAATGGSTNS
                     VLHLLAIASEAKIPLSIDDFDRISSRTPILADLKPGGRFVATDLYAAGGTALVAKRML
                     EANLLHGESLTVTGRTLAEEAADAKESEKQEVVRKLDAPLKPTGGLVILKGNIAPEGC
                     VLKVAGHNYSTFSGPARVFDNEEDAFAAVENGSIKAGDVVVIRYEGPKGGPGMREMLQ
                     VTAALIGAGLGDSVALLTDGRFSGATHGLMAGHVAPEAANGGPIAAINDGDIISFDIA
                     KRALNVKLTDAEIQRRLSTWKPPQPRFTSGVMAKYALLVSSASQGAITAVPTTFSAAA
                     QTVSRTS"
BASE COUNT          425 a          609 c          592 g          384 t
ORIGIN      
        1 gaggcgggca aaaaacatcc catcgaagtt gtcggcgggc agttgcgcgc gatgatgccg
       61 tggatgaaga agaaccgcgt gattgctgaa ggggcgtagc caacaggaag tttgtcggtg
      121 gagcgctgct accagtgctc caccctcagc aattcggcgt ctcgcggcag cctgagcaca
      181 gagagtacag acgcacgact aacatcgctt tttcgtgttg gctctggcga acagtctttt
      241 cctgcttgtt ttccgcactt ataaaactga tttcggagga ttcatggatt cgcgtctcag
      301 ggaacatagc cggatcatcg tcgacggccc ttcaagagcg ccggcgcgtt cttattttcg
      361 ggcggttggg ttgaccgatg acgatcttgc aaagcccctg gttggtgtcg caaacacctg
      421 gatcgaagcc atgccctgca attcccacct gcggcatctc gcggaaaaag tgaaggaagg
      481 aatccgcgcg gccggcggca cgcccatgga attcaatact gtcgcgatca gcgacggcga
      541 atccatgggc acggagggca tgaaggcttc gctcgtgagc cgcgaggtga ttgcggactc
      601 aatcgaattg gttgggcgag gctacatgtt tgatgccatg gtcgctctgg cgggatgtga
      661 caagacaatt cccgcgacgg caatgggatt gattcgctta aatctgccga gccttgtgct
      721 ctacggcgga tcgattcaac cgggccgatt tcgcgggcgc gatgtaacga ttcaggatgt
      781 cttcgaagct gtgggcgcgc actcggcggg gaaaattacg gatgcggacc tgaaggaaat
      841 cgaaagcgtg gcgtgtccgg gcgcgggggc gtgcggaggg cagttcactg caaacaccat
      901 ggcgatggca ctggaattta tcggactaac gcccatgggt ttgggcaacg tccctgcgac
      961 cgacccgcga aaagacgatg cggcctatcg ctccggcacc ctggtgatgg aacttcttcg
     1021 caaaggcatc acgccgcgac agattctgac acgcaaagcg ttcgagaacg cgattaccgc
     1081 ggttgccgcg acgggaggct ccaccaattc cgtcctgcat ctgctcgcca tcgccagcga
     1141 agcaaagatt ccgctatcga tcgacgattt cgaccgcatc agctctcgca cccctatcct
     1201 tgccgacttg aagccgggcg gccgcttcgt cgccaccgat ctctacgccg cgggcggcac
     1261 tgctctcgtc gccaagcgga tgctcgaagc aaatttactt cacggcgaaa gcctcacggt
     1321 caccggacgc accttggccg aagaagccgc agatgcaaag gaatccgaga aacaagaagt
     1381 cgtgcgcaaa ctcgacgcgc cactaaagcc cactggcggc ctcgtcattc tgaaaggaaa
     1441 tattgctccg gaaggctgtg tcctgaaggt cgctggccac aactacagca cgttcagtgg
     1501 ccccgcgcgc gttttcgata acgaagagga cgccttcgcc gccgtcgaga atggaagcat
     1561 caaagcgggc gatgttgttg tgattcgcta cgaaggccca aagggcggcc cgggtatgcg
     1621 cgagatgctg caggtcaccg ccgcgctgat cggtgccggc ctcggcgatt cggtcgctct
     1681 gctcaccgac ggccgctttt ccggcgcaac ccacggcctc atggccggcc acgtcgcccc
     1741 ggaagccgcg aatggcggcc ctatcgctgc tatcaacgac ggcgacatca tctccttcga
     1801 catcgccaag cgcgcgctca acgtaaagct caccgacgcc gaaatccagc gccgcctctc
     1861 cacttggaag ccaccgcaac cgcgcttcac cagcggcgta atggcgaaat acgctctcct
     1921 tgtctcctct gcatcccagg gcgcgattac cgccgtcccc acaacttttt cagccgcagc
     1981 gcaaaccgtc tcgaggacat catgaagctc
//
LOCUS       DASTPM010000530         6605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_162526, whole genome shotgun sequence.
ACCESSION   DASTPM010000530 DASTPM010000000
VERSION     DASTPM010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162526"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFR42_09385"
     CDS             complement(<1..333)
                     /locus_tag="VFR42_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369410.1"
                     /translation="MRIDDAQIRQQLSTLGVELQSGMALSTLTSLGIGGTTELLRITR
                     HESIPSILDLLDRHNIPHRFLGGGSNLLVGDGELPWVVLQLAKPDRDVFIQGNLARID
                     GAADLGRTV"
     gene            complement(323..1744)
                     /gene="murC"
                     /locus_tag="VFR42_09390"
     CDS             complement(323..1744)
                     /gene="murC"
                     /locus_tag="VFR42_09390"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU0369411.1"
                     /translation="MMVSFRNFQRIHLVGIGGIGMSGIAEVLLTLGYSVSGSDTRLST
                     LTERLQDLGATIYEGHKAGNVEGAHVVVTSSAIKSNNPEIVEAHKRKIPVIPRAEMLA
                     ELMRLKYGIAVGGAHGKTTTTSLVASILTAAHLDPTFVVGGKVNQAGSTARLGKGEYF
                     VVEADESDRSFLLFAPVVAVVTTIDREHLDQYTSLADIQDAFTQFVNRVPFYGAAILC
                     LDEPNVQSIIPNVKRPILTYGTSSQADLLIGDIELRGLASEFRLTYKGEDLGLFHLPH
                     PPGIHNVRNAAAAAAVALYLNVPVELIRQGLANFGGVGRRFDVKGSVNDITVIDDYGH
                     HPAEIKATLEAARGCKFNRLLVLFQPHRYSRTQHLWEEFTRSFNQADVLVLTDIYAAS
                     EAPIPGVTSEALAAAIREAGHKNVHYIRSMQDSIGFLLKNARRGDAILTVGAGSVSRA
                     SNELMVLLGTEHPSHHVPESHAH"
     gene            complement(1741..2856)
                     /gene="murG"
                     /locus_tag="VFR42_09395"
     CDS             complement(1741..2856)
                     /gene="murG"
                     /locus_tag="VFR42_09395"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEU0369412.1"
                     /translation="MKLLIAGGGTGGHVFPAIAIAREWMSRGSEREVVLVGTQRGIEI
                     KLVPQAGLPLETLRAAGLKGKGGLTLARNLSMLVPGTLDAFSILRKHKPVAAFGVGGY
                     AAGPMMLATWLRGIPNVIFEPNAEPGFTNKVLARLSKRIATGYEVTARGLGGKGVVTG
                     NPVRAEFFSIEPRKPQKPLHLLITGGSQGALPINRTFVDAMDLLAARKNELSIVHQTG
                     ERDYHAVRTAYARREFHSAEVVPFLGDMAERFAWADLIVCRAGAITASEIAAAGRAAI
                     FIPFGRATDSHQLRNAQEMAKAYAGKVVVESELNADRLANEIFALLDRPEQIQVIAAN
                     ARKLGRPHAARDIVNLVEEVAGVQGRSAQGDNREKIS"
     gene            complement(2876..3994)
                     /gene="ftsW"
                     /locus_tag="VFR42_09400"
     CDS             complement(2876..3994)
                     /gene="ftsW"
                     /locus_tag="VFR42_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEU0369413.1"
                     /translation="MPRRLENDRWLFIVTLGLCLLGAVMIFSASAVTAEHEYGHSYIF
                     LLRQSAWLGIGLAGMFVLMRTDYRRLREPGVVYAVLCAVGLMLVGAFFLDKSHATHRW
                     IRFGPVGIQPSELAKLAVILYLAWFLDLQRRNAAAMEFRKEDFLHNILPAIGPVLVCV
                     MLILAQPDLGTSVDIVLIAVAVLYVAGLSWKWIAVGAAASVPALYLLITHVAYRQARM
                     MAFLNPDSDPQGAGFQLLQSLIAVGSGGFTGVGLMESKQKLFYLPEAHTDFIYAVICE
                     ELGFIGALVVITLFVVYGWRGLRAAFSAPDNFGRLLALGITAMVMFQALINFAVVLGM
                     VPTKGIPLPFISYGGSSLLVMLLATGVLLNISQQATNR"
     gene            complement(3987..5402)
                     /gene="murD"
                     /locus_tag="VFR42_09405"
     CDS             complement(3987..5402)
                     /gene="murD"
                     /locus_tag="VFR42_09405"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU0369414.1"
                     /translation="MVGRIELNRRRVLVIGLARTGVATALFCAARGAVVTAIDLRSAD
                     ELGEAAGKLRGAGVRLEFGGYAPNILQRQELVVPSPGVPADAPLLQEARALGLKIWSE
                     VELASHFLYGQMIGITGSNGKTTSASLVEHILHAAGFSTVLAGNIGTPLIAHASETAD
                     HTIAVAELSSFQLELIENFEPKVAVFLNLTPDHLDRHKTLAAYGAAKARIFENQTEQD
                     FAVLNADDSASSAYAPSRPQVFWFSRKKNVEQGAFVRGGKIIFRRAGNEEEILDASEI
                     PLPGAHNLENVLAAVVATRLAGAQPSQIAAGIRSFAGVEHRIEFVAEVQGVRYYNDSK
                     ATNVDATLKALDSFPGRILVILGGKDKDSDYTQLRTALREKAILALLIGTAAEKIEKQ
                     VEGSVAIRRVGTLENAVDSAFHTAHRGDVVLLAPACASFDQFQNYEHRGRVFKELVHH
                     LEKGEKPGSHGNPVNAGKRNA"
     gene            complement(5451..6584)
                     /gene="mraY"
                     /locus_tag="VFR42_09410"
     CDS             complement(5451..6584)
                     /gene="mraY"
                     /locus_tag="VFR42_09410"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU0369415.1"
                     /translation="MLYYVLYHLLQKYFSPLNVFRYITVRTVYASLTAMFLALVFGPW
                     LIRRLRELQIGQYIREEGPQEHKKKAGTPTMGGVLIVLSTAVPVLLWADLTNPFVLLS
                     LFALLGFATIGFIDDYAKVAKRRNLGLTSKRKFLLQVLVSTGVGVALLGLSTHSAYST
                     QLIVPFIKKFHPDLVIHSLLSSPHLWPLAFAPFLIFMVIVITGSSNAVNLTDGLDGLA
                     IGCTVIAAGALTVLTYVSSNARWANYLDIQFIPRVGELTVFCGALVGASLGFLWYNAH
                     PAEVFMGDVGSLSLGGTLGVIAVIIKQEILLFFVGGIFVIEALSVILQVSSFKLRQKR
                     IFRMAPIHHHFELLGWSESKVIVRFWILALVFALFALTTLKLR"
BASE COUNT         1440 a         1935 c         1955 g         1275 t
ORIGIN      
        1 gacggtgcgg ccgagatcgg ctgccccgtc aatgcgggcg agattgcctt ggatgaacac
       61 gtctcggtct ggcttggcaa gttggagaac gacccagggg agttcgccgt cgccgaccag
      121 caagttcgaa ccgccgccca gaaacctgtg cggaatattg tggcggtcga gaagatccag
      181 gatggaagga atcgattcgt gcctggtgat gcgcagcagc tcggtagtgc cgccaatacc
      241 gagtgaggtc agcgtggaca aggccatgcc ggactgcagc tcgacaccga gcgtggagag
      301 ttgttggcga atttgagcgt cgtcaatgcg catggctctc cggcacatgg tgactcggat
      361 gctcagttcc gagcagaacc ataagctcgt tgctggcccg gctgacgctt ccggctccaa
      421 cagtaaggat ggcgtcgccg cggcgggcgt tcttgagcag gaaaccaatg ctgtcttgca
      481 tggaacgaat gtaatggacg tttttgtggc cggcttcgcg gattgcggcg gccagggctt
      541 cgctggtgac gccgggtata ggcgcttcgc tggcagcgta gatatccgtt aggaccagga
      601 catcggcttg attgaaggag cgcgtaaact cttcccaaag atgctgagtg cgggaatacc
      661 ggtgcggctg gaaaaggacc agcaggcggt tgaatttgca gccgcgggca gcttcgaggg
      721 tcgctttaat ttcggcaggg tgatggccgt agtcgtcgat caccgtaatg tcgttgacgg
      781 aacctttgac atcgaaacgg cggccgactc cgccaaagtt ggcgagtccc tgccggataa
      841 gctcgacagg aacgttgaga tagagcgcga cggcagcggc agcggcggca ttgcgtacgt
      901 tgtgaatgcc gggcggatgc gggaggtgga aaaggccaag gtcttcgcct ttgtaggtaa
      961 ggcggaattc gcttgcgagg ccgcgaagtt cgatatcgcc gatcaggagg tcggcctggc
     1021 tggatgtgcc ataagtaagg atggggcgtt tgacgttggg aatgatggac tgaacgttgg
     1081 gttcgtcgag gcaaaggatg gcggcgccgt agaacggaac ccggttaacg aactgcgtaa
     1141 aggcgtcctg aatgtcggcg agcgacgtgt attggtcgag gtgttcgcgg tcgatagtgg
     1201 tcacgacggc gactaccggc gcaaaaagca gaaagctgcg atcgctttcg tcggcttcga
     1261 ccacgaaata ttcgcctttg ccgaggcgag ccgtagatcc ggcttggttc actttaccgc
     1321 cgacgacgaa cgtcgggtcg aggtgcgcgg cggtgagaat gctggcgacc agtgaagttg
     1381 tggtggtctt gccatgcgcg ccgccgacgg caatgccgta cttgaggcgc atgagttcgg
     1441 ccagcatttc ggcgcgcggg atgacgggaa ttttgcgttt gtgagcctcc acgatctcgg
     1501 gattattgga cttaatggcc gaagaggtca cgaccacgtg cgcgccttcg acgttgccgg
     1561 ctttgtggcc ttcgtagatg gtggcgccca gatcctgcag gcgttcggta agagtggaaa
     1621 gtctcgtgtc ggagccggaa acggagtagc cgagcgtaag caaaacttcg gcgatgccgc
     1681 tcatgccgat gccgccgatg ccaacgagat gaattctctg gaagttgcgg aagctgacca
     1741 tcatgaaatt ttttccctgt tgtcgccctg cgcgcttctt ccctgcacgc cggcgacctc
     1801 ttcgacgagg ttgacaatgt cgcgagccgc gtggggacgg ccgagtttac gtgcgttcgc
     1861 agcgatgacc tgaatctgtt ccggtcgatc caggagcgca aaaatttcgt tcgctagacg
     1921 gtccgcgttc agttcggact cgacgacgac tttgccggcg taagccttgg ccatttcctg
     1981 ggcattgcgg agctgatggc tgtccgtggc gcggccaaac ggaatgaaaa tagcggctcg
     2041 cccggcggct gcgatttccg aagcggtgat ggcccccgcg cggcacacga tcaagtcggc
     2101 ccaagcgaag cgttcggcca tgtctccaag gaacggaacg acttcggcgg agtgaaactc
     2161 ccgccgcgcg taggcagttc ttaccgcatg gtaatcgcgc tcaccggtct ggtggacgat
     2221 gctgagttcg ttcttgcggg cagcgagcaa gtccatggca tccacgaacg tgcggttgat
     2281 cggcagcgcg ccttggctgc cgccggtgat gagcaggtgc agcggctttt gcggcttgcg
     2341 cggctcgatg gagaagaatt cggcgcgtac ggggttgccc gtgaccacgc ccttgccgcc
     2401 aaggccgcgg gccgtaactt catagccggt cgcgatgcgt ttcgagaggc gagcgagaac
     2461 cttgttggta aagccgggtt ccgcgttcgg ctcgaaaatc acgttgggaa tgccgcgcaa
     2521 ccacgtggcg agcatcatgg ggccggccgc gtaacctccg acgccaaaag cggcgacggg
     2581 tttgtgcttg cgcaggatcg agaaagcatc cagcgtcccc ggtaccagca tggataaatt
     2641 cctggctagc gtcaggccgc ctttgccctt cagtcccgcg gcgcgcaaag tttctagcgg
     2701 cagcccggcc tgcggcacca gttttatttc gattccgcgt tgcgttccca ccagcacaac
     2761 ttccctttcg cttccgcgcg acatccattc gcgcgcgatg gcaatggccg gaaagacatg
     2821 gcctcccgtg ccgccacccg cgatcagcag tttcatcgaa caaatccgcc tgcgctcatc
     2881 ggttggtcgc ctgctgagag atattcagca gcactccggt agccagcagc atcaccagca
     2941 aacttgagcc gccataactg atgaacggga gcggaattcc tttcgtcggg accatcccca
     3001 acaccaccgc aaagttgatc agcgcctgga acatcaccat cgccgtgatg ccgagcgcca
     3061 gcaggcggcc aaaattgtcg ggtgcggaaa acgccgcgcg cagcccgcgc cagccgtaca
     3121 cgacgaacaa tgtaatcacg acgagcgctc cgatgaagcc cagttcttcg cagatgacgg
     3181 cataaataaa atcggtgtgc gcctcgggga gatagaaaag cttctgcttg ctttccatca
     3241 agccgacgcc ggtgaagccg ccggaaccga ctgcgatgag agattgcaac aactggaatc
     3301 ctgcaccttg cgggtcgcta tccgggttga ggaacgccat catgcgcgct tggcgatagg
     3361 caacgtgggt gataagaagg tagagcgcgg gaacagatgc ggctgcgccg acggcaatcc
     3421 atttccatga caagccggcg acatacagca cagcgaccgc gatcagcaca atgtccaccg
     3481 aagtgccaag gtccggctgc gctaaaatca gcattacaca caccagcacc ggaccaatcg
     3541 cgggcagaat gttgtgcagg aaatcttcct tgcgaaactc catcgcagcg gcgttgcggc
     3601 gctggagatc caggaaccac gctaaataaa ggatgacggc gagtttggcg agttccgagg
     3661 gttgtatgcc aaccggcccg aagcgaatcc accgatgcgt cgcatgcgac ttgtcgagaa
     3721 aaaatgcacc gaccagcata agcccgaccg cgcacagcac ggcgtacacc acgccgggtt
     3781 cgcgcaggcg gcggtaatcg gttcgcatca agacaaacat gccggccagg ccaatgccga
     3841 gccatgcgga ctgccgcagc agaaaaatgt acgagtggcc gtactcatgt tccgctgtga
     3901 ccgccgaagc gctgaaaatc atgacggcgc cgagcagaca cagccccagg gtgacgatga
     3961 acaaccagcg atcgttttcg agccgcctag gcattgcgtt tccccgcatt caccggattc
     4021 ccgtgcgaac caggcttttc gcccttttcc agatggtgca ccagctcttt gaagacgcgg
     4081 ccgcggtgtt cgtaattctg aaactgatcg aagctcgcgc aggccggcgc gagcagcacg
     4141 acatctccgc gatgagctgt gtgaaaggcg ctgtccactg cgttttccag cgtgccaacg
     4201 cgccgaatgg ctacgctacc ctcaacttgt ttttcgattt tttccgccgc ggtgccaatc
     4261 agcagagcaa gaatcgcctt ctcgcgcagc gccgtacgaa gttgcgtgta gtcgctgtct
     4321 ttgtcttttc ctccgagtat caccaggatg cgcccgggga aggaatcgag cgccttcaga
     4381 gttgcatcta cattcgtcgc cttggagtcg ttgtagtagc gtacaccttg aacttccgcg
     4441 acgaactcga tgcgatgctc gactccggcg aaggagcgaa tgccggcagc gatctgcgac
     4501 ggctgcgcgc ccgcaaggcg cgttgccaca accgcggcaa gcacgttttc cagattgtgc
     4561 gcgcccggca gcggaatttc actggcatca agaatttctt cctcgttgcc agcgcgtcgg
     4621 aagatgatct ttccgccacg aacgaatgct ccctgctcca cattcttctt gcggctgaac
     4681 caaaacactt gcgggcgaga cggcgcatac gccgagctgg cgctatcgtc ggcgttcaag
     4741 accgcaaagt cctgctcggt ttgattctcg aaaatgcggg ctttagcggc tccataagcc
     4801 gccagcgtct tgtgtcggtc caggtgatct ggcgtcaaat tcaagaacac cgcgaccttc
     4861 ggctcgaaat tttcgatcag ttcaagctgg aagctgctta actccgccac agcaatcgta
     4921 tggtcggcgg tttcgctggc gtgcgcaatc aggggcgtac cgatattgcc cgcgagcacc
     4981 gtggaaaatc cagcggcgtg cagaatgtgc tcgacaagcg acgcggaagt cgtttttcca
     5041 ttggagccag tgataccgat catctgcccg tacaaaaagt ggctggccag ttcgacttcg
     5101 ctccagatct tcaagccaag cgcacgcgct tcttgcaata aaggtgcatc tgcgggcaca
     5161 cctggactgg gtacgaccaa ttcctgcctt tgcaaaatgt tcggagcgta gccaccaaac
     5221 tccaggcgaa cgccggcacc gcgaagtttt cccgcggctt cgcccaactc gtccgcactg
     5281 cgcaaatcaa ttgccgtcac gacagcgcca cgcgccgcgc agaacagcgc cgtcgcgacg
     5341 cccgtgcgcg ccagtccgat caccagcacg cgcctacggt tcagttcgat ccttccaacc
     5401 atcttggccc cggcccccag gtcttaacgc aagacttccc cccaacgctc ttacctcagc
     5461 ttgagcgtcg tgagcgcaaa cagcgcaaac accagcgcca gaatccagaa tctcacaatg
     5521 accttagatt cgctccaccc cagcaattca aaatgatgat ggattggcgc catccggaaa
     5581 atgcgttttt gccgcaattt gaacgaactc acctgcaaaa tcaccgacag tgcctcaatc
     5641 acaaaaattc cgcccacaaa aaaaaggagg atctcctgct tgatgatcac agcaatcacg
     5701 ccgagcgtcc cgcccagcga cagcgaacca acgtcgccca taaacacttc cgcgggatgt
     5761 gcgttgtacc agagaaagcc caatgacgcg cctaccagcg cgccgcagaa caccgtaagt
     5821 tcaccgacgc gtggaataaa ctgaatgtcc agatagttgg cccaccgcgc gttgctactg
     5881 acgtaagtca gcacagtgag cgcgcccgcg gcaatcaccg tgcatccgat cgccaggcca
     5941 tccagcccgt ccgtcaaatt caccgcgttc gaagaacccg taataacaat caccatgaaa
     6001 atcagaaatg gcgcgaacgc caggggccac aggtgcggac tgctcagcag tgagtgaatc
     6061 accaggtccg ggtgaaactt ctttatgaac ggaacgatca gctgcgttga atacgcgctg
     6121 tgcgtagaaa gcccgagcaa tgcaacgccc acgccagtgc tcaccagcac ctgcagtaaa
     6181 aactttcgct tgctggtcaa acccaagttt cgcctcttcg cgaccttggc gtagtcatca
     6241 ataaaaccga tggtcgcaaa tcccaggaga gcaaataaag agagcagcac gaagggattg
     6301 gtgaggtcgg cccagagcaa cacgggtacg gccgtcgaaa gaacaatcaa cacgccgccc
     6361 atagtcggcg ttccagcctt ctttttgtgc tcctgcggcc cttcttcgcg gatgtactgc
     6421 ccaatctgca gttctcgcag ccgccggatc agccacggcc cgaacaccag cgccagaaac
     6481 atggcagtca gactggcata cacggtgcgc accgtgatgt agcgaaacac gttcagcgga
     6541 ctgaaatatt tctgcagcag gtggtagagc acgtagtaca gcatctagtg gctgacctcc
     6601 gctgc
//
LOCUS       DASTPM010000531         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1287283, whole genome shotgun sequence.
ACCESSION   DASTPM010000531 DASTPM010000000
VERSION     DASTPM010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287283"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1942)
                     /locus_tag="VFR42_09415"
     CDS             complement(<1..1942)
                     /locus_tag="VFR42_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369416.1"
                     /translation="MEEFGKERADNSPEMVSENAIHSGEELRKRRSSRIVQAVPLTVK
                     GVDALGRPFIERTSSLIINCHGCRYQSKHYVLKNMWVTLEIPHPETGQPPRQVRGRVA
                     WIQRPRTVRQLFQVALELELPGNVWGIGFPPEDWTSFGIPSAPSRVHAMHEDEIHAVA
                     AEKTAEHTGAPGEPEMPPPAIGVDTSLHLMPAPASTTDASLQLARQVARLIADAKQQI
                     SAAAREAAAHAVTTEHRTASEQWEQKFSAARSELANETGRSIERVQQKSGEWSQAAQA
                     AATKALQEELPKWLSPHLRELTRELTVALSKTGSEQRALHEQQITSAAEKVQQLCREA
                     EDVARRIVAQAQDAERRLSEHASVTSAVLESQTKKQEDTFSAHHERLNATAGSAQQQI
                     TEAIANAEAQLKSQFDEKVEASKAQLQEFLEGAMARLNEHTVQLLGQRGEELRARLLE
                     NLNSEAEQRMATLRESSQGLVVEVQQLIQSLRAELQSQAGLLDHATERAGKTAFQLDQ
                     LASQLGTTQQLAIESFQSQLDDVLSLHRNELHRRSESLFEELNERIRRSFDEASSAAV
                     SKFDEQVRGLVEPQISQTNDALHRLAGGKSLLDAALTLQQDRIRNSADEAFAESLGRF
                     RENLGGVEQLLHDASQGVIARNL"
     gene            complement(1988..2317)
                     /locus_tag="VFR42_09420"
     CDS             complement(1988..2317)
                     /locus_tag="VFR42_09420"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEU0369417.1"
                     /translation="MPGLAHYMAQYDHEHSSGWNKLLHGIGIPLIFAGIILLILTKWI
                     WGGALFVGGWMLLFLGHKVEGNHPAFFQGPIYLLVGPIWVAKEAWTFLTGSHAQADSP
                     AEKTGHR"
     gene            2706..2852
                     /locus_tag="VFR42_09425"
     CDS             2706..2852
                     /locus_tag="VFR42_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369418.1"
                     /translation="MSALGFSLLQGDIPRSIRLWRIKCARGSLWSVYGCWRCVRAEHA
                     ITRE"
     gene            2871..3116
                     /locus_tag="VFR42_09430"
     CDS             2871..3116
                     /locus_tag="VFR42_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369419.1"
                     /translation="MKERTKVRALPLWWRTILYLTLLAAMLTNRYLRLQNGGTPDSFF
                     TTAFLLQDAVAELTDFPAVCAPNSSARLSSLRSHSSA"
     gene            complement(3234..3881)
                     /gene="recR"
                     /locus_tag="VFR42_09435"
     CDS             complement(3234..3881)
                     /gene="recR"
                     /locus_tag="VFR42_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU0369420.1"
                     /translation="MYRQFAPCNRGVLHKVMPDFAAPVERLIDELKHLPGIGQKTAQR
                     LAFHLLRCAPEDALALADAIRDAKEKIRECTTCGNITDNDPCLYCAGASRNKRTICVV
                     EEAHNILTIEKTRTYSGMYHVLGGSLSPLQGRGPEQLKIKHLIERLKGGSVEEIIIAT
                     NPTAEGEATAVYLSKLLKPLGVRVTRIGVGIPVGADLEYADEITMLKAMEGRREL"
     gene            complement(3889..>4801)
                     /locus_tag="VFR42_09440"
     CDS             complement(3889..>4801)
                     /locus_tag="VFR42_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369421.1"
                     /translation="IFRKPNLFNNNSIGLPRAFLPASLQEAPMSNLLATNQAKEVSLD
                     PALVPLICATLLYAVSWIMGCSSPATSARKSSPAKTVEAREQLRLASDDASDKLAPSI
                     SGRKLTIEKMAQRAATSLQFSNEEYGVAFDAPKGYLLKEGELSERDRGLGYLGPVPMH
                     FTQEGGVRLATVEPPTGVLLGTNFVNEFFTISALYNSTAADCSALDIAPEFRGARVNR
                     TVDGIVFRGTEEVAVASMHQYTGIYLHGFANETCYEIGYGVATIGDGPARNLKHVDPQ
                     KLMSRLEKILDTVRIAPPNFERFTATD"
BASE COUNT          856 a         1348 c         1493 g         1104 t
ORIGIN      
        1 tcaaattgcg cgcgatcact ccctgcgaag catcgtgcaa caactgctcg acgccgccga
       61 gattttcgcg gaaccggcca agagattccg cgaacgcttc gtcggcggaa ttgcggatgc
      121 gatcctgctg cagtgtaagc gcggcatcca gcaatgactt gccgcccgcc agccggtgca
      181 gcgcgtcatt ggtctggctg atctgtggtt ccaccagccc gcgcacttgc tcgtcaaact
      241 tgctcactgc cgctgagctc gcctcgtcaa acgatcgccg gattctctcg ttcagttctt
      301 caaacaacga ttcgctgcgg cggtgtaatt cgttgcggtg cagggacagg acgtcgtcca
      361 gttgcgattg aaagctctcg atggccagtt gctgggtggt tcccagctgc gaagcgagtt
      421 gatccagttg aaatgccgtt ttgccggcgc gttccgtcgc gtgatcgagc aggcccgcct
      481 ggctttgcag ttccgcgcgc agcgactgaa tcagttgttg cacttcgact acaagtccct
      541 gcgaactttc gcgcaacgtc gccatgcgtt gttccgcttc gctgttgaga ttctctagca
      601 acctggcgcg cagctcctcg ccgcgctggc ccaacagctg gaccgtatgt tcattcagcc
      661 gcgccatggc gccctccagg aactcctgaa gttgcgcttt gctagcttcc actttttcgt
      721 cgaattgtga tttcaactgc gcttcggcgt tcgcgatggc ttccgtgatt tgctgttgtg
      781 cggagcctgc ggtcgcattc agccgttcgt ggtgagcgct gaacgtgtct tcctgttttt
      841 ttgtctggct ctccagcacc gccgaggtaa cagaggcgtg ttcgctcaac cggcgttccg
      901 catcctgcgc ttgcgccacg atgcgacgag ccacgtcttc tgcttcgcgg cacagttgct
      961 gcactttttc cgcggcgctg gtgatctgct gctcatgcag agcacgttgc tcggatccgg
     1021 tcttggaaag cgcgacagtc agctcgcgcg tcagttcgcg caagtgcggc gacagccact
     1081 tcggcaattc ttcctgcaaa gctttcgttg cggcggcctg cgccgcctga ctccactccc
     1141 cacttttttg ctgaactctt tcgatggagc gtcccgtttc gttggccagt tcgctgcgcg
     1201 cggcggagaa tttctgctcc cactgttcgc tggcggtacg atgttccgtg gtaacggcgt
     1261 gagcagcggc ttcgcgtgcc gccgcgctga tctgctgttt tgcatcggcg ataagtcgcg
     1321 cgacttgccg cgccagttgc agcgaagcat cggtggtact ggcgggcgca ggcatcaggt
     1381 gaagggaagt atccacgccg atcgcgggtg gcggcatttc cggttcgccc ggcgcgcctg
     1441 tgtgttctgc ggttttttcc gcggccaccg cgtgaatttc gtcttcgtgc atcgcatgca
     1501 ctctggatgg cgccgatggg attccgaaac ttgtccaatc ttctggcgga aacccaatgc
     1561 cccagacgtt tccgggcagc tccagttcca gcgcaacctg aaaaagctgg cgcacggtgc
     1621 gtggccgctg gatccaggct acccgtccgc gaacttggcg cggcggctgg ccggtttccg
     1681 ggtgcggaat ctccagcgtc acccacatgt ttttcaggac gtagtgtttc gactggtagc
     1741 ggcagccatg gcagttgatg atcaggctgg aagtgcgctc aatgaacggg cggcccagcg
     1801 cgtccacgcc cttgacggtg agggggacgg cttgcacgat gcgcgagctg cgtcgcttgc
     1861 gcagttcttc gccgctatga atggcgttct cggagaccat ctctggagaa ttgtcggcac
     1921 gctctttgcc aaactcttcc atgcccgacg ttctttccct ttgtgtatct ctcgtgttgt
     1981 tcgcggctta gcggtggcca gttttctctg ccggagaatc tgcctgggca tgggaaccag
     2041 tgaggaaagt ccatgcctct tttgccaccc aaatcggacc aaccagtaag tagatgggtc
     2101 cctggaaaaa ggcggggtga ttgccctcca ccttgtggcc caaaaacagc agcatccagc
     2161 cgcccacaaa cagcgcgccg ccccagatcc acttcgtgag gatgagcaat atgatccccg
     2221 caaaaatcag cgggattccg attccgtgca acagcttgtt ccaaccggaa gagtgctcgt
     2281 gatcatactg cgccatgtag tgcgccaatc cgggcatcta gcgcacctcc ttgcgggaca
     2341 ttccgcggaa ccaccgtctt tcgggcggcg ggtcttcttg cagaattctg actctatacc
     2401 gaaaaaacta aaatggctag attgagctag gagtactgcc gggtcgctct agtacgtgcc
     2461 tgttgcaatt catctaccgt tactctcctg ggagatcgat tcggtatcct ctcccgtccc
     2521 ggttcgacgc tagacaacat tggcaggggt attgcgatga gcgaatgtcc ggcaatccgc
     2581 gaaggctata agccaccgct ggtcgcttgc gggtcgtctg gcaagtagcc atcggcttac
     2641 aggattcccg gacctggatc gtggcggtag ttggcacggt tggcagcatc gcaggaggag
     2701 gacccatgag cgccctcggt ttctcacttt tgcaaggcga cattcccagg tctattcgct
     2761 tgtggcgcat caaatgcgct cgcggctctc tctggagtgt ttatggctgc tggcgctgtg
     2821 tgcgcgccga gcacgccatc acccgcgaat gagagcatga cttccttggc gtgaaggaga
     2881 gaacaaaggt acgggcactc ccgctctggt ggcggaccat tctttaccta acgctgctgg
     2941 cagccatgtt gaccaaccgc tacttgcgct tgcagaacgg cggcactccg gacagcttct
     3001 tcacgacggc gttcctgcta caggacgctg ttgcggagct aaccgatttt cccgccgtat
     3061 gcgcgccaaa ctccagtgca agattgagca gcctccgttc gcacagctcg gcgtaatctt
     3121 cgccgctgga atgagctggt agcatttgct cttttccgta gccattaggc tttagtaaaa
     3181 aaaaacgaaa acaaaaagga aaacatccgt agaggctgcg tttcccgccg cctctacaac
     3241 tcgcggcggc cttccatggc tttgagcatg gtgatttcgt cggcgtattc aaggtcggcg
     3301 cctacgggga tacctacgcc gatgcgcgta acgcggacac ctagcggctt cagcaatttc
     3361 gagagataga cggcggtggc ttcgccttcg gcggtggggt tggtagcaat gatgatttct
     3421 tcgacggaac cacctttgag gcgctcaatc aagtgcttga ttttcagttg ctccggaccg
     3481 cgaccctgga gcggggagag agagccgccg agcacgtggt acatcccgct gtaggtgcgg
     3541 gttttttcga tggtcagaat gttgtgggct tcttcgacga cgcaaatggt gcgcttgttg
     3601 cgcgaagcgc cggcgcagta gagacacggg tcgttgtcgg ttatgttgcc gcaggtagtg
     3661 cattcgcgga ttttttcttt ggcgtcgcgg atggcatcgg cgagggcgag cgcatcttcc
     3721 ggtgcgcagc gcagcaaatg gaaggccagg cgctgcgcgg ttttttgccc gattccgggc
     3781 aagtgtttca gttcatcgat gaggcgttcg acaggggcgg caaaatcggg catgaccttg
     3841 tgtagcacac cgcggttgca cggcgcaaat tggcggtaca tcgctggctt agtcggtggc
     3901 ggtgaagcgc tcgaagttgg gcggcgcgat gcggacggtg tcgagaattt tttcgagacg
     3961 ggacatcagt ttttgcggat cgacgtgctt caagttgcgg gcggggccgt cgccaatggt
     4021 agcgacgccg tagccgattt cgtagcaggt ttcattggcg aagccatgca ggtagatgcc
     4081 ggtgtattgg tgcatgctgg cgacggcgac ctcttcggta ccgcgaaaaa cgatgccgtc
     4141 gacggtgcgg ttgacgcgcg cgccgcgaaa ttccggagcg atgtccaaag cagagcagtc
     4201 tgcagcggtg gagttgtaga gcgcgctgat ggtgaaaaat tcgttgacga agttggtacc
     4261 caggagaacg ccggtaggcg gctcgacggt ggcgagacgc acgccacctt cctgggtgaa
     4321 gtgcatcggc acggggccga ggtagccgag gccgcgatcc ctttcggaga gttcgccttc
     4381 cttgagcaga tagcctttgg gagcgtcgaa cgcgacgccg tattcttcgt tggaaaattg
     4441 cagcgaggtg gcggcgcgct gcgccatttt ttcgatggtg agcttgcggc cggatatgga
     4501 cggcgcgagt ttgtcgctgg cgtcgtccga tgcgagccgc aattgttcgc gagcctcgac
     4561 ggtctttgcc ggagaagatt tgcgcgcgct ggtggcgggg ctggagcagc ccatgatcca
     4621 tgagacggcg tagagcaggg tggcgcagat gagcgggacg agagctgggt cgagactgac
     4681 ctctttggct tggttcgttg cgagcaagtt ggacatggga gcctcctgca gggacgcagg
     4741 gagaaaggca cgaggcagtc cgatggaatt gttgttgaaa agattgggct tacgaaaaat
     4801 t
//
LOCUS       DASTPM010000532         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_105097, whole genome shotgun sequence.
ACCESSION   DASTPM010000532 DASTPM010000000
VERSION     DASTPM010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105097"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..715)
                     /gene="rpsF"
                     /locus_tag="VFR42_09445"
     CDS             complement(305..715)
                     /gene="rpsF"
                     /locus_tag="VFR42_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU0369422.1"
                     /translation="MEDRLYDLIFIIRPATPEDEIKKVEHVVETACSEKGGKIEKTEH
                     WGTRKLAYRVAKHREGIYVYFQIRTNHGELIHEIERRLRVQDSVIKYLTIRLDEDIKR
                     QKKLSGIREKRAARRPKRPAPSPTQPAEQQATAS"
     gene            complement(778..1371)
                     /gene="pth"
                     /locus_tag="VFR42_09450"
     CDS             complement(778..1371)
                     /gene="pth"
                     /locus_tag="VFR42_09450"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU0369423.1"
                     /translation="MRLIVGLGNPGPEYQWTPHNLGFLAVDELANRGGIRVERPEAKA
                     LVGLGRVAGEEVILAKPQTFMNLSGISVKELLAKYEVGPENLLAMWDEAQLPWGTIRI
                     HAEGSAGSHNGAKSLIGSLSTMAFTRLRLGCGPDHPLSSRKEYVLRPMKKAELEVAAE
                     MIADAGDAVELLLTKGIEAAMTKYNRRERPESEDAAS"
     gene            complement(1421..2122)
                     /locus_tag="VFR42_09455"
     CDS             complement(1421..2122)
                     /locus_tag="VFR42_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU0369424.1"
                     /translation="MADKIVVEAAPRDSRGKNEARRLRVTGTVPAVLYGGKGDAMALS
                     VNTKQVSQILRSATGHNTLFQVAIAGKEQPAIVKDWQVDPVTGNLLHVDLLRVAMDVR
                     MRVNVPVHTFGEPAGVKVQGGIFETVTREVEIECLPGEIPTEFRLDVSHLMIGMHLRA
                     GEIQIDPNKMKLVTDPERVIAHVVALKAEEEKPAEVAAEGAVPAEPEVIKKGKKEVEG
                     EEGVEAPAEKQEKKK"
BASE COUNT          411 a          728 c          695 g          545 t
ORIGIN      
        1 cgggccacca ccggggccgc cttcacgacg tccgccgcca ccggggccgc cttcgcggcg
       61 agggccggca ctgtggccgc cttcgtggcg aggcgcctgg tgcggcgcgg aggcctgcgg
      121 agccgcgggc ttcgattccg gcgccgctgg cggtgcttga ttctgttcgt ctgccataac
      181 tttttaccct tttccatgcc agggtttgta aaacaaaccc tggaaccctt tctttcagaa
      241 actcttcacg gcgaaccggg gatgccggaa cgcctggatt tcgcaaaatg gccgcggcag
      301 aaaactagct ggcggtggct tgctgctcgg ccggctgcgt cggagaaggc gcaggacgct
      361 tgggacggcg cgcggcgcgc ttttcacgaa tgccagaaag tttcttctgg cgcttgatgt
      421 cttcgtcgag gcggatggtc aaatatttga tgaccgagtc ctgcacgcga agccgccgct
      481 cgatttcgtg gatcagctcg ccatgattgg tgcgaatctg aaaatagacg tagatgcctt
      541 cgcggtgctt ggccacgcga taggccagct tgcgcgtgcc ccagtgctcg gtcttttcga
      601 tcttgccgcc cttttcggag caggcggttt cgaccacgtg ctcgactttc ttgatttcgt
      661 cctccggtgt ggccggacgg atgataaaaa tcaggtcgta cagacgatct tccatgtttc
      721 ctcgtttcct tactcgcggc gggataaacc caccgttaca actaaaccca attctcttta
      781 gctcgcggcg tcttcgcttt ccggacgttc gcggcggtta tacttggtca tcgccgcttc
      841 gatccccttg gtcaggagaa gttcgaccgc gtcgcccgcg tcggcgatca tttcggcggc
      901 gacttccagc tccgcttttt tcatcggccg caggacgtat tccttgcggc tggaaagcgg
      961 atgatcggga ccgcaaccga gccgcagcct ggtgaacgcc atcgtgctca gagaacctat
     1021 gagcgacttg gcgccattgt ggctgccggc gctgccttcg gcgtggatgc gaatcgtgcc
     1081 ccagggcaac tgcgcctcgt cccacatcgc gagcaaattt tccgggccca cctcgtactt
     1141 cgcgagcagc tcctttaccg agatgccgct caggttcatg aaggtttgcg gcttggcgag
     1201 gatgacttct tcccctgcca ctcttcccaa acctaccaga gccttcgctt ccggccgttc
     1261 gacgcggatg ccgccccgat tggccaattc atccacggcg agaaatccga ggttgtgcgg
     1321 cgtccattgg tattccgggc caggattgcc aaggccgaca atgagccgca tatgcctcgc
     1381 tccgatacct gcgcaggcaa gattgcctgc gctacaaaag ctacttcttt ttctcctgct
     1441 tctcggcagg agcttcaaca ccttcctcgc cttccacttc cttcttgccc ttcttgatga
     1501 cttctggctc ggccgggact gcaccttccg ccgcaacttc ggcaggcttc tcttcttccg
     1561 ccttgagagc gacgacgtga gcgatgacgc gctccggatc ggtgaccagt ttcattttgt
     1621 tcgggtcgat ctggatttca ccggcacgga ggtgcatgcc gatcatcagg tggctgacgt
     1681 cgaggcggaa ctcggtggga atttcgcccg gcaagcattc gatttcgact tctcgagtga
     1741 cggtttcaaa gatgccgcct tgtaccttga cgcccgcggg ttcgccaaag gtgtgcaccg
     1801 gaacgttgac gcgcatacga acgtccatgg cgacgcggag aaggtccacg tggagcaggt
     1861 tgccggtgac gggatcgacc tgccaatcct tcacgatggc gggttgctcc ttgccggcga
     1921 tagctacctg gaacaaggtg ttgtgcccgg tggcggagcg gagaatctgg ctcacctgct
     1981 tggtgttgac gctgagcgcc atcgcatcac ccttgccgcc gtacaggacc gcgggaactg
     2041 tgccggtgac gcgcaaacgg cgagcttcat tcttgccgcg agaatcgcgg ggagctgctt
     2101 cgactacgat cttgtctgcc attgccttct ttccttttca tgtcagggtt tgtaagtaca
     2161 aaccctgaaa cccttttcca aaaaatcagc acagcgaaag tcttagataa acagttcgct
     2221 gatcgaggtt tcctcgtgaa tcgagcgaat gccgcgagcc agcaaatcgg ccacgctcag
     2281 cacgcgaatc tttttcaacg cgcggcccgc ttcgctgagc ggaacggagt cggtggcgac
     2341 gacttcggtg atgcgcgagg cggcgatgcg ctccacagc
//
LOCUS       DASTPM010000533         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1287339, whole genome shotgun sequence.
ACCESSION   DASTPM010000533 DASTPM010000000
VERSION     DASTPM010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287339"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="VFR42_09460"
     CDS             complement(<1..1570)
                     /locus_tag="VFR42_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369425.1"
                     /translation="MSRTAQDVLEFDKLRELLRQRATCILGRRAVDALAFSQDISALQ
                     QQFALIREAREWLRSARDLGFGALSDPETWLAKIEGPSAALEPAEFLEAALLLEAASW
                     LRLQFREEAGQFPLLTERAAKVADFRDALASIRRCLLPNGEISDDASATLRRIRVSIV
                     QTRDGIQRSLKQILRSRNAEAGEDYVTLRNDRFVIPVRAENRRTVPGVVHGASSTGQT
                     VFLEPFETVEANNQLVQLAEDESAEILRILRELTARLQRELPALLAAAETIQLLDGIF
                     ARGRFARDFDAALPEFSASGELRLESARHPVLEDKLNREQRAIVPMTLSLNRDERLLV
                     ISGPNTGGKTVALKTTGLAALAAQCAIPVAAQRAVLPLFDHVLVDIGDEQSIAADLST
                     FSAHMLNLKSILETATPQSLVLVDEMGTGTAPEEGAALAVALLDEFRAKECIVLATTH
                     HDRLKSYAAAVPGVVNAAVEFDDVNLSPTYKLIVGVPGGSSGIAIAQRLGVAKQIIDR
                     ARELLSPESREAADL"
     gene            complement(1567..2109)
                     /locus_tag="VFR42_09465"
     CDS             complement(1567..2109)
                     /locus_tag="VFR42_09465"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEU0369426.1"
                     /translation="MERIITVFGSSRPREDHADYQEARLLGRALAEAGFAVCSGGYAG
                     VMEAASRGAKEAGGKTYGVTAEFFKPKANAWVDTEIRVTTWQERLFELVRVADGFVTC
                     KGGTGTLVELAVVWEMLNKAVIGNKPIVVLGEFWTPILDRVREVEVGHPGPWGEANGK
                     LVHTAITPLEAAEFLSKKLA"
     gene            complement(2129..>3671)
                     /gene="dacB"
                     /locus_tag="VFR42_09470"
     CDS             complement(2129..>3671)
                     /gene="dacB"
                     /locus_tag="VFR42_09470"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU0369427.1"
                     /translation="VTAQQTIHPSGMPAPEKKGSAAHEGHAMLPAELFAARAQLLLNN
                     GQPSKGDWGLLVADAKTGEVLFEQNADRYFVPASNMKLFTTALALATLGPDFRFRTTL
                     ESLVSPSRDGKLTGPLFLVGRGDPNLSNRKLPYAQKEEFDGPPERAIAELADALVGKG
                     IKEINGDIVGDDSYFPRDRYPNGWEIDDMVWEYGAAISAIVVDDNTVQLTLTPGEKAG
                     DHVNAIVNPSTPEFVVDNQVVTSAAGVKPDLTLKREPGANLVTVLGTLPAKSNPRKLT
                     LAIQEPALHAAAMLKRLLEERGIRVDGVARELSVSPAAPPDQRVVLAEHLSIPLGESV
                     KLVNKISQNLHTEMLLRTAARQKGLWTTPDELAAFPSEFYAVAGIPPGDVVQSDGSGL
                     SRHDLVTPRAIVAVLLYAQKQPWFGDYFVSLPVAGIDGTLEDRMKNSIAAGRLHAKTG
                     SVEHVRTRSGYADLPSGRRLVFSFMSNNMGSKGHEATDALDALSIAMIEEFDAQGSAC
                     CKRTP"
BASE COUNT          707 a         1069 c         1153 g          742 t
ORIGIN      
        1 tcaggtcggc ggcttcgcgc gattccggtg aaagcagctc tcgcgcgcgg tcgatgattt
       61 gttttgccac gccgaggcgc tgggcgatgg cgataccgct ggaaccgccg ggaacgccaa
      121 cgatcagttt gtacgtggga ctaagattga cgtcgtcgaa ttcaaccgcg gcgttgacaa
      181 cacccggcac cgccgcggcg taggacttca agcgatcatg gtgcgtggta gcgagcacga
      241 tgcattcttt agcgcgaaat tcgtcgagaa gcgcgacggc gagcgctgcg ccttcttcgg
      301 gggcagtgcc ggtacccatt tcgtccacga gaacaagcga ttgcggagtg gcggtttcca
      361 gaatggattt gagattcagc atgtgcgcgg aaaaagtgga gaggtcggca gcgatggact
      421 gctcgtcgcc gatgtccacc agaacatgat caaacagagg cagaacggcg cgctgcgcag
      481 cgacgggaat cgcgcactgg gccgcgaggg cagcaaggcc agtggttttc agcgccacgg
      541 ttttgccgcc ggtattgggt ccgctgatga cgagcaggcg ttcgtcgcgg ttgagcgaaa
      601 gcgtcatcgg gacgatagca cgttgttcgc gatttagttt gtcctccaga acagggtgac
      661 gcgcagattc gagccgcaat tcgccgctgg cagaaaactc aggcaaggcc gcgtcaaaat
      721 cgcgcgcgaa tcggccgcgt gcaaaaatac cgtcgagcaa ctgaatggtt tccgccgcgg
      781 ccaggagcgc cggcaactcg cgctgcaatc gcgcagttaa ttcgcggagg atgcgcagga
      841 tttccgccga ttcgtcttca gcaagctgaa ccagctggtt gttggcttca acggtttcga
      901 atggctccag aaagactgtt tgtccggtgg agctggcgcc gtgcaccaca ccgggcacag
      961 tcctgcgatt ttccgcgcgc acgggaatta caaagcggtc gttacgcagg gtgacgtagt
     1021 cttcgccagc ttcagcgttg cgcgagcgca agatctgttt caaggaccgc tgaatgccgt
     1081 cgcgggtttg cacgatgctg acgcgaattc ttcggagcgt agctgatgcg tcatcgctaa
     1141 tttcgccatt aggcaggagg cagcgccgga tcgaggcgag ggcatcgcga aaatccgcga
     1201 ccttcgcggc gcgctccgtt agcaatggaa actgccccgc ttcttcgcga aattgaaggc
     1261 gcagccagga cgccgcctcc aagagcaaag cagcttcgag aaattccgcc ggctcgagtg
     1321 cagcactcgg tccttcgatt ttggcgagcc aggtttccgg atcggacaaa gcgccaaagc
     1381 cgaggtcgcg agcagaacgc agccattcgc gcgcttcgcg aatcaacgca aattgttgtt
     1441 gcagcgcaga aatgtcttgc gaaaacgcca gagcatccac ggcgcggcgg ccgaggatac
     1501 acgtggcgcg ctgccgcagc agttcgcgca gcttgtcaaa ttccagaacg tcttgcgcag
     1561 ttcgactcat gccagtttct tgctcaagaa ctcagccgcc tcgaggggcg ttatcgcggt
     1621 gtggaccagt ttgccattgg cttcgcccca cgggccgggg tggccgactt cgacctcgcg
     1681 aacgcgatcg aggatgggcg tccagaattc tcccaggacg acaatgggct tattaccgat
     1741 gacggccttg ttcaacattt cccatacgac tgcaagttcc accagcgtgc cggtgccgcc
     1801 tttgcaggtg acaaagccgt cggcgacgcg cacaagctca aacaatcgct cttgccaggt
     1861 ggtcacgcga atttccgtgt cgacccacgc gttggccttg ggcttgaaaa attccgcagt
     1921 gacgccgtag gtttttccgc cggcttcttt ggcgccgcgt gatgccgctt ccatcacgcc
     1981 ggcatagccg ccgctgcaga cggcaaagcc cgcttccgcg agcgcgcgtc ccagcaagcg
     2041 tgcttcctga tagtccgcgt gatcttcgcg cggacgcgaa ctgccaaaga ccgtgatgat
     2101 gcgttccatc gtggcgattt ccgaaaagtt atggcgttct tttgcagcag gccgagcctt
     2161 gagcgtcgaa ttcctcgatc atggcgatgc tcaatgcgtc cagcgcatcg gtggcttcgt
     2221 gtccttttga acccatgttg ttgctcataa aggaaaaaac aagacgcctg ccgctgggca
     2281 ggtcggcgta gcctgaccgc gtgcgcacat gttcgacgga gccggtcttg gcgtgcagcc
     2341 ggcccgcggc aatgctgttc ttcatgcggt cttccagcgt tccgtcaatg cccgctaccg
     2401 gcaatgaaac gaagtaatcg ccgaaccacg gctgcttctg cgcgtaaagc aacactgcga
     2461 cgatggcgcg gggcgttacc aaatcgtgcc gcgataatcc ggagccgtcg ctctgcacta
     2521 catcgcccgg cggaattccc gctactgcat aaaattccga cgggaaggcc gccagctcat
     2581 cgggcgtggt ccacaaaccc ttttggcgcg ccgcggtacg cagcagcatt tcagtgtgaa
     2641 ggttctggct gattttgttg accagcttca ccgactcgcc gagcggaatc gaaagatgct
     2701 cggccaggac gacgcgttga tcgggaggcg ccgcgggaga gacggaaagc tcgcgcgcca
     2761 cgccatctac tctgatgccg cgttcctcga gaagtcgttt caacatggcg gccgcgtgga
     2821 gcgccggctc ctgaatggcc agcgtcagct tgcgcggatt gcttttcgcg ggcagcgtgc
     2881 ccagcaccgt caccagattc gcgcccggtt cgcgtttcag ggtgagatca ggcttcactc
     2941 cggcggcgga ggtgacgacc tggttgtcca ccacaaattc gggcgtcgaa ggattgacga
     3001 tggcgttcac gtggtcgcca gccttttctc caggcgtcaa tgtgagttgc acggtgttgt
     3061 cgtccaccac gatcgcggaa attgccgcgc cgtactccca caccatgtcg tcaatttccc
     3121 agccgttggg gtaacggtcg cgcggaaagt agctatcgtc gccgacgatg tcgccgttaa
     3181 tttccttgat gcctttgcct accagcgcat cggccagttc ggcgatagcg cgttcgggtg
     3241 ggccgtcaaa ttcctccttc tgcgcgtagg gcagcttacg gttggagaga ttggggtcgc
     3301 cacggccgac gaggaacaga gggcccgtta attttccgtc gcgtgacggc gaaacgagcg
     3361 actcgagagt ggtgcggaag cggaagtccg ggcctaacgt cgccagggcg agcgcggtgg
     3421 tgaacagctt catgttggaa gcgggaacga aatagcggtc ggcgttttgt tcgaagagaa
     3481 cttcgccggt tttcgcgtcg gcgaccaata atccccagtc gcccttggaa ggctgtccgt
     3541 tgttgagcag aagctgcgct cgcgcggcga agagttccgc ggggagcatg gcgtggcctt
     3601 catgcgccgc cgagcctttc ttttcgggtg caggcatgcc ggacggatga atcgtctgtt
     3661 gcgccgttac g
//
LOCUS       DASTPM010000534         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_681892, whole genome shotgun sequence.
ACCESSION   DASTPM010000534 DASTPM010000000
VERSION     DASTPM010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681892"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFR42_09475"
     CDS             complement(<1..604)
                     /locus_tag="VFR42_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369428.1"
                     /translation="MHSQQDQSQRPEGATLLFARGVTLAALGIFLFGAREAVADPLPQ
                     EPQGEIIGTIEGQAIALKGPMSVQVVGNEVKTLLRSGVDIRVKLGRARIDLAEGGTIA
                     VCGPAHISMLKAGHALTIALDSGTIHAHVEGNLTLNVFTAQILAKTVAIGDGPQDILA
                     GFDAPGLMCVRANRGALRLEEQFGSQSVMVPQGGDVLLTNG"
     gene            712..1536
                     /locus_tag="VFR42_09480"
     CDS             712..1536
                     /locus_tag="VFR42_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0369429.1"
                     /translation="MTMKTAHQQTNGSGSAEAAAAAEPEPQSQKLGVHYDTQLPKLPW
                     SRRIQIPIIAALVYSVIRILGPTLRFEVLGWQSAENVYASGRRCIWAFWHRVIIPVVW
                     WRRNCGVVVMNTTAFDGQWTRKVIEWMGYGTAQGSSSRGGLRGLAVMARRIEQGHDCA
                     FTIDGPRGPRYVAKPGPVMLARRTGAPVVVFHIGVEHGKTFEKTWDHFLLPRLFTKTI
                     MIFAPPIEVPPDADAALLEAKHAEMQKELERVRDVAEDWFSLSEVERERFRKELGK"
     gene            complement(1588..>2150)
                     /locus_tag="VFR42_09485"
     CDS             complement(1588..>2150)
                     /locus_tag="VFR42_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0369430.1"
                     /translation="QNTLIDEAIQTDAAINRGNSGGPLINTHGEVIGINSAIYTPTGT
                     TAGIGFAIPINTAKRIAHDLITDGRVHQAFIGVETIPINESFAEALGLPAQEGLLVQT
                     TTRGGPAAEAGIKGGDRVAQAGMRRFYIGGDVITAIDGQKVSSPLDVNLVLNRKRPGD
                     TVSVSLFRGGKKMDVPVKLGERPGNN"
BASE COUNT          432 a          620 c          652 g          446 t
ORIGIN      
        1 ggccattcgt aaggagcacg tcgccgcctt gtggcaccat cacgctttgg ctgccgaatt
       61 gttcctcgag acgcagtgcc ccgcggttag cgcgaacaca catcaggcca ggagcatcga
      121 aaccagcgag gatatcttga ggtccatcgc cgatcgcgac ggtcttcgca agaatctgcg
      181 cggtaaagac gttcagcgtg agattgcctt cgacatgtgc gtggatggtg ccactgtcga
      241 gagcgatggt cagcgcgtga cctgctttca acatggaaat gtgcgcgggg ccgcagaccg
      301 cgatggttcc accttcggcg aggtcaatgc gcgcgcggcc cagcttcacg cggatatcga
      361 cgccactgcg cagcagcgtc ttcacctcgt tgccgactac ctgcacgctc atcggaccct
      421 tgagggcgat ggcctggcct tcaattgtgc cgataatctc gccttgcggc tcttggggga
      481 gtgggtcggc taccgcttca cgagcgccga agagaaaaat gccgagcgct gctagcgtga
      541 caccgcgcgc aaacaggagc gttgctcctt cagggcgctg agattggtct tgctgtgagt
      601 gcatattgaa ggcaaagagc cgccgggccg ctctcttctc tatactatac gtccagcgga
      661 ttttcgccgc acagggaaaa gggtttcagg gttgtgctta caaaccctga tatgacgatg
      721 aagaccgcgc accaacagac aaacggatcg ggcagcgcag aggctgctgc cgcagcggag
      781 cccgaaccgc agtcgcaaaa gctgggtgtg cattacgata cgcagcttcc gaaacttccc
      841 tggtcgcgcc gcattcagat tcccatcatc gccgcgctgg tctactccgt gattcggatt
      901 ctagggccga cactccgttt tgaggtgctt ggctggcaaa gcgcggaaaa tgtatacgcc
      961 tcgggacgaa gatgcatctg ggcgttttgg caccgggtga tcattcctgt ggtttggtgg
     1021 cggcgcaatt gcggcgtggt ggtgatgaac acgacggcgt tcgacggaca atggacccgg
     1081 aaagtaatcg aatggatggg ctatggaacc gcgcagggaa gttcgtcgcg tggcggattg
     1141 cgcggcctgg cggtgatggc acggcgaatc gagcaggggc acgattgcgc gttcacgatt
     1201 gacggtccgc gcggaccacg ttatgtggcc aagcccggac cagtcatgct ggctcgacgt
     1261 accggagcgc ccgtcgtcgt gttccacatc ggcgtcgaac acggaaagac atttgagaaa
     1321 acctgggacc atttcctgct gccgcgactt ttcacaaaaa caatcatgat tttcgcgccg
     1381 cccatcgaag ttccgccgga cgccgatgcc gctttgctcg aagccaagca cgccgaaatg
     1441 cagaaagaac tggagcgcgt ccgcgacgtt gcagaggact ggttctcact cagcgaagtg
     1501 gagcgcgaaa ggttccgcaa ggagcttggg aaataatagg cacagcagca tcgcaccgtg
     1561 caattccgtt gattttcaga tggacggcta gttgttgcca ggtcgctcgc cgagcttcac
     1621 cggaacatcc atttttttgc cgccgcggaa taggctgaca ctcacggtat ctccaggccg
     1681 ctttctgttg agcaccagat tcacatccag gggacttgaa accttctggc catcgatggc
     1741 ggtgatcaca tcgccgccaa tgtaaaaacg ccgcatgccg gcctgcgcca cgcggtctcc
     1801 tcctttgatc ccggcctcgg ccgccggtcc gccgcgcgtc gtggtttgca ccagcaaacc
     1861 ctcttgcgcg ggaaggccca gcgcttccgc aaaagattca ttgatcggga tcgtttccac
     1921 gccaataaac gcctggtgca cgcggccatc ggtgatcaga tcatgcgcaa tcctcttggc
     1981 ggtgttgatg ggaatcgcga agccgatgcc ggccgttgtg ccggtcggtg tgtagatcgc
     2041 cgaattgatg ccaataactt cgccatgcgt gttgatcagg gggccgccgg aattgccgcg
     2101 attgattgca gcatccgtct gtatcgcttc atcgatcagc gtgttttgcc
//
LOCUS       DASTPM010000535         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1374028, whole genome shotgun sequence.
ACCESSION   DASTPM010000535 DASTPM010000000
VERSION     DASTPM010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374028"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VFR42_09490"
     CDS             <1..431
                     /locus_tag="VFR42_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369431.1"
                     /translation="MEGIAAVTHIAGKPAPAIVKERIPNATYCEPQIGSIGLTEKQAR
                     DAGYAVKTGKFPFVGNSKATILGNHGGFIKVVSEEKYGEVLGIHIIGPLATEILAEAA
                     AVLHLEGTIDDMMNMMHAHPTVWEGMGDAFASVRGLQINV"
     gene            445..1371
                     /gene="lipB"
                     /locus_tag="VFR42_09495"
     CDS             445..1371
                     /gene="lipB"
                     /locus_tag="VFR42_09495"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEU0369432.1"
                     /translation="MTSDRKNPNFTGGAGCEREASTSHESPVTGHRNSPCLTIDLGLI
                     GYAEAYALQKRVVAARKAGAIDDVLLLCEHPHVITQGRNGKREHLLAGENVLRQKGIS
                     LYETSRGGDITYHGPGQIVGYPILNLAAIRRDVVWYVRMLEEVMLRASADFGVNAIRE
                     PGKTGIWVSAENSLPREKSISEKLGAIGVHISRWVTSHGFAYNVSTDLRFFDLIIPCG
                     IAGRKATSLEKLLNRRVNSQEVAVRLTHHFGQVFAREMQSATPQEFLQRLEACEQARE
                     QSSAPLKTPATEATAQPQFAALPATEIQQPHR"
     gene            1382..>2111
                     /locus_tag="VFR42_09500"
     CDS             1382..>2111
                     /locus_tag="VFR42_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU0369433.1"
                     /translation="MSTAATGASMKKDLTRQQYLDLYYYMRLNRAVEDTRVKLFRQNK
                     IVGGLYSSLGQEGISVGSAYALEKKDWLAPMIRNIGALLVKGVPPRDVFMQHMAKYDS
                     PTKGKDGTSHFGDLDNLHIVSPISMLGDLIPVMAGVAIAGRYLGQKIVAMTWIGDGGS
                     STGVFHEGLNFAATQKAPFVLILENNLWAYSTPVRRQVPLENLADRAKAYGIASYVVD
                     GNDVVAMYKTAKEAVDRARAGEGPI"
BASE COUNT          473 a          747 c          539 g          352 t
ORIGIN      
        1 tgatggaagg tatcgccgcc gtcacccaca tcgccggcaa acccgcgccc gccatcgtca
       61 aggagcgcat ccccaacgcc acctactgcg agccgcagat cggctccatc ggcctgaccg
      121 agaagcaagc ccgcgatgcg ggttacgccg taaaaaccgg aaagtttcct tttgtcggca
      181 acagcaaagc caccatcctc ggcaaccacg gcggcttcat caaagtcgtt tccgaggaaa
      241 agtacggcga agtgctgggc atccacatca tcggcccgct ggccaccgaa atcctggccg
      301 aagccgccgc cgtcctgcac ctcgaaggca ccatcgacga tatgatgaac atgatgcacg
      361 cgcatcccac cgtctgggaa ggcatgggcg atgccttcgc ctccgtccgc ggattgcaga
      421 ttaacgttta gcgactggtg attagtgact agcgatcgga aaaacccgaa tttcaccggc
      481 ggcgctggat gcgagcgcga ggcttccacc agtcacgagt caccagtcac tggtcaccgt
      541 aacagtccct gcctcaccat cgatctcgga ctcatcggct acgccgaagc atacgcgctg
      601 caaaagcgcg tcgtggccgc gcgcaaagcc ggcgcgatcg acgacgttct attgctctgc
      661 gaacatccgc acgtaatcac ccagggccgc aacggcaagc gcgaacatct gctcgccggc
      721 gaaaacgtcc tgcgccaaaa aggtatctcg ctctacgaaa ccagccgcgg cggcgacatc
      781 acctatcacg gccccggaca aatcgtcggc taccccatcc tcaatctcgc tgccattcgc
      841 cgcgacgtcg tctggtacgt gcgcatgctc gaagaagtaa tgctccgcgc ttccgcggac
      901 tttggcgtaa acgcaatccg cgagcccgga aagaccggta tctgggtatc ggcggaaaat
      961 tcactgccgc gcgaaaaaag catttccgag aagctcggtg ccatcggcgt acacatcagc
     1021 cgctgggtca cctcccacgg ctttgcctac aatgtctcca ccgacttgcg ctttttcgac
     1081 ctcatcatcc cctgcggcat cgccggacgc aaagccacct ccctcgaaaa actcctgaac
     1141 cgccgcgtca attcccaaga ggttgccgtc cgcttgacac atcatttcgg tcaagtcttc
     1201 gcgcgtgaaa tgcaatccgc cacgccgcaa gagttcctgc aaaggctaga ggcgtgcgag
     1261 caagcccgcg agcagtcgag cgcgccgctc aagaccccag caaccgaagc taccgcgcag
     1321 ccacaattcg ccgcgctgcc cgcaactgaa atccagcaac cgcatcgttg agcacaaaac
     1381 catgagcacc gccgccaccg gagcaagcat gaagaaggac ctgacccgcc agcaatatct
     1441 cgacctctac tactacatgc gcctcaaccg cgcggtggaa gacaccaggg tcaagctctt
     1501 ccgccagaat aaaatcgtcg gcggccttta ctccagcctt ggccaggaag gcatttccgt
     1561 cggcagcgcc tacgccctcg agaaaaaaga ctggctcgcc cccatgatcc gcaacatcgg
     1621 cgcgctcctg gtcaaaggcg ttcccccgcg cgacgtcttc atgcagcaca tggccaagta
     1681 cgattcaccc accaagggca aagacggcac cagccatttc ggcgacctcg acaatctcca
     1741 catcgtctcg cccatctcca tgctcggcga cttgatcccc gtcatggccg gcgtggctat
     1801 tgccggccgc taccttggcc agaaaattgt cgccatgacc tggatcggcg acggcggcag
     1861 ctccaccggc gtcttccacg aaggcctgaa ttttgccgcc acgcaaaaag cccccttcgt
     1921 cctcatcctc gaaaataatc tctgggccta ctccactccc gtccgccggc aggtgccgct
     1981 cgaaaacctc gccgatcgcg ccaaagccta cggcatcgcg agttacgtcg tggatggcaa
     2041 cgacgtcgtc gccatgtaca agaccgccaa agaagccgtc gaccgcgccc gcgccggcga
     2101 aggccccatc c
//
LOCUS       DASTPM010000536         5524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_306930, whole genome shotgun sequence.
ACCESSION   DASTPM010000536 DASTPM010000000
VERSION     DASTPM010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306930"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1176)
                     /locus_tag="VFR42_09505"
     CDS             complement(190..1176)
                     /locus_tag="VFR42_09505"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HEU0369434.1"
                     /translation="MKKTICMLALALFCTGVASAAKGYSYFRVGNTSDVTTATTAGTV
                     LMGGGTDVDAAFQWMCQRSGNGDFLVIRATGTDAYNPYIQQLCPNENSVATLIIPNRT
                     AASDPFVISAIQNAEALWIAGGDQSNYINFWRGTPVQTALQGLIARSVPIGGTSAGMN
                     VLSQFIYSALASQGVTSSQALADPFTRYITLDRDFVSIATVTGLIGDPHFVTRDRMGR
                     DLAFLCRIFLNGWSATPRGISIDEQTALLIDGSGNGTVVGASTVYFMQAPGAPQVCQS
                     KTPLTYQNISVYRIVAGGTFNLSTWTGHNGVNYTVSANAGVLSSTQSGGSIY"
     gene            complement(1265..2233)
                     /gene="egtD"
                     /locus_tag="VFR42_09510"
     CDS             complement(1265..2233)
                     /gene="egtD"
                     /locus_tag="VFR42_09510"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEU0369435.1"
                     /translation="MIVQAPSQKIVSEFAEEVRAGLSKRNQRELPSKYLYDEVGSELF
                     EAICLLPEYGLTRADTRLLQKYAEEMVARMPTPTHVAELGRGSGKKTRMILEALAKRR
                     RTFYYPIEISPAALAACEKELGQLDFVSLVGYEKPYLEGLRAVAARREPGEHVCVLFL
                     GSTIGNFDRPAADDFLRQVRAVLQPGDSLLLGTDFEKDVVLQLPAYDDPVGVTAAFNL
                     NLLARVNRELGGNFQLRDFRHLALWNEEERRIEMHLQATRRQQIEIPSASLYFQLEEG
                     ETIWTESSHKYSPDEPAQMAERAGFRQIAQWTDDEWPFAQNLWIVD"
     gene            complement(2274..3602)
                     /locus_tag="VFR42_09515"
     CDS             complement(2274..3602)
                     /locus_tag="VFR42_09515"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0369436.1"
                     /translation="MGALASAIRTRHEYQLRLAHARSTTDQLFEILREEALYERPIAE
                     RHRVVFYVGHVEAFDWNLLGQRAFGLKRFHPTFDQLFAFGIDPVGGGLPSDKPEEWPA
                     RGQVDAYNRRLREELDRAIEKTLANSSAGHPQFLAMLDTAIEHRLMHAETLAYMFHRL
                     PAKMKKAPRAEQRWMAPQIRHRMVEIEAGPATLGLPRDSSQFGWDNERGMVTVTAPEF
                     AIENHNVTNRQFLLFMQNGGYRDRSLWAAADWAWREANGIEHPAFWYRDGSIWMQRGM
                     FGDMRLALEHPVYVSHAEATAYANWLGRKLPTEEQFHRAAYGTPGSNVQRAYPWGEAA
                     PAPHYGNFDFAHWEAAPCGAHPAGASAFGIHDLVGNGWEWTRTIFAPHPGFAPMPFYP
                     GYSANFFDGKHFVMKGGSPRTAACMLRRSFRNWFQPHYPYVYATFRCVEE"
     gene            3741..4502
                     /locus_tag="VFR42_09520"
     CDS             3741..4502
                     /locus_tag="VFR42_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0369437.1"
                     /translation="MTKPIIEFRHVEYLISTIPSRSILSDVSLQIHPGETIALLGRSG
                     SGKTTLLKLINGLRFPSSGTLQIQDRSVRDWDLIQLRRSIGYVIQEVGLFPHLTVREN
                     VCLVPQLENWPADRISSRYVEVLQSVSLSPAVYSNRYPRELSGGQRQRVGVARALAAD
                     PKILLMDEPFGALDPVTRAELQREFLRLAAHMANTVVLVTHDVREALLLATRIVLLDS
                     GRIIAIAAPSEFARLTHPVAREFLAAAELSTGASS"
     gene            4499..5131
                     /locus_tag="VFR42_09525"
     CDS             4499..5131
                     /locus_tag="VFR42_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369438.1"
                     /translation="MNAWQFMAGHRAEILSATREHVLLVLISMAIAVAIGVPLGLVIV
                     RHPRLRAVSLAVASILQTIPSLALFGFLIPLPFLGGIGAHTAIVALVLYALLPILRNT
                     FVGLTSIDPAILEAAEGMGMTQQQILFRVRLPLASSFIMAGIRTATVITIGIATIAAA
                     IGAGGLGTFIFRGVAMVSDAVILAGAIPAAILALFADFLLGLLERRLRVT"
     gene            5134..>5524
                     /locus_tag="VFR42_09530"
     CDS             5134..>5524
                     /locus_tag="VFR42_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0369439.1"
                     /translation="MPRVLVSCLLALALCSGACRSPQKPHLVVSSKFFTEQVILAELL
                     AQHIEARTGIPVTRKANLGGTLLVHKAMLSGDVDLYVEYTGTALTAVLGEKPEGDSNA
                     IYQRVRDEYAHRFSLEVTEPLGFENTFA"
BASE COUNT         1052 a         1786 c         1426 g         1260 t
ORIGIN      
        1 tcggagaatt tctggattgc gcccattgaa accgtttccg aatcggaaga cggctttgag
       61 cgggtctatc agggatcgca gattttggcg cgctggcgac tcagcactca gaagatcctt
      121 tctgcgcgcg tggtctggcg cctggaaacc atctgaactt cttgatgctg gatttttttg
      181 acgaatggtt cagtagatcg atccgccgga ctgagtcgaa ctcagcaccc ctgcgttcgc
      241 ggacacggtg tagttcactc cattgtgtcc tgtccaggtt gacagattaa atgtcccgcc
      301 cgcaacgatt cgatacacgc tgatgttctg gtaggtgagc ggtgttttgg attgacaaac
      361 ttgtggcgca ccaggcgcct gcatgaaata gacggtgctg gcacctacta ccgtgccgtt
      421 gccgctgcca tcaatcagca acgcggtttg ctcatcgatt gaaatgcccc gcggcgtggc
      481 ggaccatcca ttcagaaaaa tccggcacaa aaatgccaaa tcgcgcccca tgcggtctcg
      541 cgtcacaaaa tgcggatctc caatcaatcc agttacggtc gcgatgctca caaaatctcg
      601 atccagcgtg atataccgcg tgaagggatc ggccagcgcc tggctcgaag tcacgccctg
      661 gctcgccagc gccgaataaa tgaattgcga aagcacattc attcccgcgc tggttccgcc
      721 gatcggaacc gaccgcgcga tcagcccctg caacgccgtt tgcaccggtg tgcccctcca
      781 gaaattaatg tagttcgact ggtccccacc cgcgatccac agcgcttccg cattttggat
      841 cgcgctaatc acaaacggat cgctcgccgc cgtgcggttc ggaatgatca gcgtcgccac
      901 cgaattttcg ttcgggcaaa gctgctgaat atagggattg tacgcgtccg ttcccgtcgc
      961 tcgaatcacc agaaaatcgc cgttgccact gcgctggcac atccactgaa aagccgcgtc
     1021 cacatcggtg ccgccgccca tcagcaccgt tcccgcggtc gtcgccgtcg tgacgtccga
     1081 ggtattcccc acacgaaaat aggaatagcc tttggctgcg gaagccacgc ctgtgcaaaa
     1141 cagcgccagc gccaacatac aaattgtctt tttcattccg tcacctgagt aagaagagca
     1201 gcgcgcggcg catcatagcg ccgcaacttc ccctatggca acaggaacta ttggcaggaa
     1261 cgagctaatc cacgatccac aaattttgcg cgaacggcca ctcgtcgtcg gtccattgcg
     1321 ctatttgccg gaagcccgcg cgctccgcca tttgtgcggg ttcgtcgggc gaatacttgt
     1381 gtgagctttc cgtccagatg gtttcaccct cttccaactg aaaatacagc gacgcgctcg
     1441 gaatttcgat ctgctgtctt cgcgtagcct gcaagtgcat ttcgatccgg cgctcttctt
     1501 cgttccacaa ggccaggtgg cggaaatcgc gcagctgaaa attgccgccc agctcgcggt
     1561 tcacccgcgc cagcaaattc aaattgaacg cagcggtcac tccgactggg tcgtcgtacg
     1621 caggaagctg caacactacg tctttttcaa agtcggttcc cagtaaaagc gaatcgcccg
     1681 gctgcagcac ggctctaact tgccgcagaa aatcgtccgc cgctggacga tcgaaattcc
     1741 cgatggtgct gcccagaaaa agtacgcaaa cgtgttcgcc cggctcgcgg cgcgccgcta
     1801 ccgcgcgcag cccttccaaa tatggtttct cgtagcccac caggctcacg aaatccagtt
     1861 gtcccagttc tttttcgcac gccgccagcg ccgctggcga aatttcgatc ggataataaa
     1921 aagtgcgccg ccgcttcgcc agcgcttcca ggatcatacg cgtctttttg ccgctgccgc
     1981 gtcccagttc cgccacgtgc gtcggagtcg gcatccgcgc caccatttcc tccgcgtatt
     2041 tttgcagcag ccgcgtatcg gcgcgggtca gcccgtattc cggcagcagg cagatcgcct
     2101 caaacaattc cgatcccact tcatcgtaaa gatatttgct gggcagttcg cgctggttgc
     2161 gtttgctgag tccggcgcgc acctcttcgg caaactccga gactattttc tgcgaagggg
     2221 cttgcacgat catctcgact ccctgcggcc ctgggtgacc tgtttcgttc aatttattcc
     2281 tctacacaac ggaaggtcgc gtagacgtat ggataatgcg gttgaaacca gttgcggaaa
     2341 gagcgtcgca gcatgcaagc ggcggtgcgc ggtgagccac ccttcatcac aaagtgcttt
     2401 ccgtcaaaaa aatttgccga ataaccggga taaaacggca tcggggcaaa tcccggatgc
     2461 ggcgcaaaaa tcgttcgcgt ccattcccag ccgtttccga ccagatcgtg aatgccaaac
     2521 gcgctcgcgc ccgcagggtg cgctccgcaa ggcgccgctt cccagtgtgc aaaatcaaaa
     2581 tttccataat gcggggcggg tgctgcttcc ccccacggat aggctcgctg cacgttcgaa
     2641 ccgggcgtgc cgtatgcggc acggtgaaat tgctcttcgg tcggcaattt ccggcccagc
     2701 caattcgcgt acgcggtcgc ttccgcatga cttacgtaca ccggatgttc cagcgccaac
     2761 cgcatgtctc caaacattcc acgctgcatc cagatacttc cgtcgcgata ccagaacgcc
     2821 gggtgttcga tcccgttcgc ttctctccac gcccaatccg cggccgccca caacgagcgg
     2881 tcgcggtatc cgccgttctg catgaacaac aggaactgcc ggttcgtcac attgtggttc
     2941 tcgatcgcga attccggcgc ggttaccgtt accatgcccc gctcgttgtc ccagccgaat
     3001 tgcgatgaat ccctcggcaa tcctagcgtc gccggccccg cttcgatctc caccatcctg
     3061 tgccggattt gcggcgccat ccatcgctgt tccgctcttg gcgctttttt catcttcgcc
     3121 ggcaggcggt ggaacatata cgccagcgtt tccgcgtgca ttagccggtg ctcgatcgcc
     3181 gtatccagca tggccagaaa ttgtggatgc cccgcgcttg aattcgccag cgttttttcg
     3241 atggcccggt ctagttcctc gcgcagccgc cggttatatg catccacttg tcctcgcgct
     3301 ggccactctt ccggtttgtc ggagggtaga cctcctccga cagggtctat cccaaacgca
     3361 aagagctgat cgaatgttgg gtggaaccgc ttcaatccga acgcccgctg acccagcagg
     3421 ttccaatcga atgcttccac gtgccccaca taaaaaacca cgcggtggcg ttcggctatt
     3481 ggccgttcat agagcgcttc ttcgcgcaaa atttcaaaca gttgatcggt ggtgcttcgg
     3541 gcgtgcgcca gccgtaattg atactcgtgg cgggttcgga tggcactggc gagtgctccc
     3601 atggtcaata tcccttcctt tgatgcggct tccttgagaa ggatgcgccg aattttcggc
     3661 gcgggaggcg ctggctaagt taggatgttg acgctgcggc gcggacgctg tcaaccaaaa
     3721 caatacgaaa agttctcgac gtgactaaac ctatcatcga gtttcggcat gtggaatacc
     3781 taatttccac tattccttcg cgctccattc tttctgacgt ttctcttcaa attcatcctg
     3841 gcgaaacaat tgctcttctc ggccgcagcg gctccggtaa gactaccctc ctcaagctca
     3901 tcaacggcct gcggttcccg tcttcgggca ccctgcaaat tcaagaccgt agcgttcgcg
     3961 actgggacct catccaactc cggcgctcca tcggctacgt cattcaggaa gtcggcctgt
     4021 ttccccactt aaccgtgcgt gaaaacgttt gcctcgtgcc ccaactcgag aattggcccg
     4081 cggatcgcat ctcttcccgc tacgtcgaag tcctgcagtc ggtcagcttg tctcctgcgg
     4141 tctattccaa tcgctatccg cgcgagcttt ccggtggcca acgccagcgg gtcggcgtag
     4201 cccgtgccct cgctgcggat ccaaagatcc ttctgatgga cgaacccttc ggggccctcg
     4261 atcctgtcac ccgcgccgaa ctccagcgcg agttcctccg gctggccgcc cacatggcga
     4321 acaccgtcgt tctggtcacc cacgacgttc gcgaagccct tctgctggcc acccgcatcg
     4381 tgcttctgga tagcggccgt atcattgcca tcgcggctcc cagcgaattt gcccgcctca
     4441 ctcatcctgt ggcgcgcgaa tttctcgccg ccgccgagtt gtctaccgga gcctcctcgt
     4501 gaatgcgtgg caattcatgg ccggccatcg cgccgaaatt ctctcggcta cccgcgagca
     4561 cgttcttctc gttctgattt ccatggccat cgccgtggcc attggcgttc cgctggggct
     4621 cgtcattgtg aggcatccga ggctgcgcgc ggtttcgctc gcggtcgcca gcattctgca
     4681 gactattccc agcctcgcgc tatttggctt tctgattccg ctacccttcc tcggcggcat
     4741 cggagcgcac accgccattg tggcgctcgt tctctacgct ctcttgccca ttctgcgcaa
     4801 cacctttgtc ggtcttacct cgatcgatcc cgctattctg gaagccgccg aaggcatggg
     4861 catgacccaa cagcaaattc tttttcgtgt acgcctgccg ctggccagca gcttcatcat
     4921 ggccggcatt cgcaccgcta ccgtcatcac catcggcatt gccaccatcg ctgccgcgat
     4981 cggagccggc ggcctcggca catttatttt tcgtggtgtg gccatggtga gtgacgccgt
     5041 tatcctcgcc ggcgcaattc ctgccgcaat cctcgctctc tttgcagatt ttctgctcgg
     5101 ccttctcgaa cgccgcttgc gggtcacctg acaatgccgc gcgtactggt ttcatgcttg
     5161 ctggctctgg cactctgttc gggcgcttgc cgctcgccgc aaaagccgca cctggtcgtc
     5221 agctccaagt ttttcaccga acaggtcatc ctcgccgaac ttctagcgca gcacattgaa
     5281 gcgcgcaccg gcattcccgt cacccgcaaa gcaaacctcg gcggcactct tctcgttcac
     5341 aaggccatgc tttccggcga cgtcgatctg tacgtcgaat acaccggcac ggcactaacc
     5401 gcagttttag gagaaaagcc cgaaggcgat tcaaacgcca tctatcagcg tgtgcgcgac
     5461 gagtatgcac accgtttttc cctggaagtc acggaaccgc tcggcttcga aaacactttc
     5521 gcca
//
LOCUS       DASTPM010000537         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_622442, whole genome shotgun sequence.
ACCESSION   DASTPM010000537 DASTPM010000000
VERSION     DASTPM010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622442"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..942)
                     /locus_tag="VFR42_09535"
     CDS             complement(220..942)
                     /locus_tag="VFR42_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369440.1"
                     /translation="MATSPALSARLRDRRFYLFITILTAALVFAGFARTFYLNGLFAK
                     MHLPSLFIVHGVVFSSWLVVLVTQAFLVSARKIQIHKKLGYASMAIVVAMLVLGWIMS
                     VQAAQRGFTPPGGPPPLAFLAFQLFGLFAFAALIAAAYLLRNRPETHKRLMVGATILL
                     LTPAVARIFFLFTTSAVLPKTLAVQLGLLLACMAYDLFTRKRVHVAYFWSTAAFLLFV
                     FGAIFGGATRPWMAIAHWITGV"
     gene            complement(1027..>2843)
                     /locus_tag="VFR42_09540"
     CDS             complement(1027..>2843)
                     /locus_tag="VFR42_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0369441.1"
                     /translation="MRRQQPNRNQASNPSEIELDLGRYQLRRHGRPVQLARKPMELLL
                     FLVSRREQLVAREEIVAKLWRSDLFVDTESAVNNIVRKIRVALGDVSEKPRFLETVIG
                     KGYRFIGPVHVIHARHLTADASSSSPRVAVLAESKRTSLAVLPLAILGNSADDNGLGL
                     GFADALIARLGNLNGLDVLPTSTVLPESPSASPADVAQRLDVRFVIRGAMQSAKDQWH
                     LSVEMFDAQLQRVSYEHNGQLDLNRLFESQDQIAKNIATTLDRQLGPATAQARPRYSE
                     DPLAYADFMRGFRLSSGGDAARLDEATRHLDAAVARDPSFSLAHATLSVVCATRHFEF
                     DPARVWLEKAELHCQRALEIDPDLAEAHVARAFLLWGPSKNFQHLDAIAELKRALGLQ
                     KNLPHAYNRLGTILAHIGLLDLAREMYQRGSLYHPKRAVSHSIVQAYMWSGDYELASE
                     RIQQWRAETPGNKYPLYFAPQAALMKGKWKQAQALLDQALKFLPDEPMIFSLQGLFFA
                     LTGKKQAALKCMARACENPRTFGHAHHTYYQIACILSVLGKRQAAFEWLDRSVATGFA
                     CWPLFVKDPCLKNICDLPEFQSLITSLQTKYPDSLGLL"
BASE COUNT          631 a          693 c         1012 g          507 t
ORIGIN      
        1 atgcgcaggc ccgctaattc tttgccggtc gccgcatccc gccagagtgc agtgacgggg
       61 tcgctcatga gcactacaca ggcctgtccg caagacgttt gacgaggaac gtgacgacca
      121 gcactgcgac cgacggggcc acgacaacgg cgaaaatcag cttgcaccgt ttcgcagcga
      181 accgtcaggg acaaatgcca cagcgggggt ctccgatctt cagacgccgg tgatccagtg
      241 ggctatggcc atccagggac gagtggcgcc gccgaagatg gctccgaaga cgaacaggag
      301 gaaggcggcg gtgctccaga agtaggcgac gtggacgcgc ttacgggtga agagatcgta
      361 agccatgcag gcaagcaaga ggccgagctg gacggccaag gtcttgggca acacggcgct
      421 ggtggtgaag aggaaaaaga tgcgggcgac ggccggtgtc aaaagaagga tggtcgctcc
      481 gaccatgagg cgtttgtggg tttccggacg attgcgcaag agataagcgg cggcgatgag
      541 agcggcaaag gcaaagagac cgaacaattg aaaggcgagg aaagccaggg gtggcggacc
      601 tcccggtgga gtgaatccgc gctgggccgc ttgcaccgac atgatccagc cgagaactag
      661 catggcgact acaatggcca tgctggcata gccgagtttt ttgtgaatct ggatcttcct
      721 ggcggaaacc aggaacgcct gggtgaccag gacgaccagc caactggaaa agacgacccc
      781 atgcacgatg aaaagcgacg gcaggtgcat cttggcgaag aggccattca gataaaaggt
      841 gcgcgcgaag cctgcgaaga ccagcgccgc ggtaagtatg gttatgaata gatagaagcg
      901 gcggtcgcgc agacgggcgg atagagccgg gcttgtggcc atagttcgaa tattctccgt
      961 cagaaattat ggcgaatttt atcacggacg gcgcgagctt gaagtgcggc agcggagtgg
     1021 ccgctgttac aggaggccga ggctgtcggg gtacttggtc tgcagcgacg taatgagcga
     1081 ctgaaactcg ggaaggtcgc agatgttttt cagacagggg tcttttacaa agagcggcca
     1141 gcaggcgaag ccggtggcga cgctgcgatc gagccattcg aaggcagcct gacgtttacc
     1201 gagcacggag agaatgcaag cgatctgata gtaggtgtga tgcgcgtggc cgaaagtcct
     1261 ggggttttcg caggctcttg ccatgcactt gagggcagcc tgctttttgc cggtaagagc
     1321 gaagaaaagt ccctgcaaac tgaagatcat cggctcatcc ggcaagaact tgagcgcctg
     1381 atcgagtagc gcttgggctt gcttccactt ccctttcatc aacgcggcct gcggagcgaa
     1441 gtaaaggggg tacttattgc ccggagtctc cgcgcgccac tgctggatgc gttcgcttgc
     1501 cagttcgtag tcgccgctcc acatgtaagc ctggacgatg ctgtggctga ccgctcgctt
     1561 cggatggtag agactgccgc gctggtacat ttcgcgcgca agatcgagca gaccgatgtg
     1621 ggcaaggatg gtgccgaggc gattgtaggc gtgagggaga tttttctgca ggccgagagc
     1681 gcgcttgagt tcggcgatgg cgtcaagatg ctgaaaattc ttggatggtc cccacagaag
     1741 aaaggcgcgg gcaacgtgag cttcggcgag atcgggatcg atttcaagag cgcgctggca
     1801 atgaagttcg gccttttcga gccagacgcg cgcgggatcg aattcgaaat ggcgggtagc
     1861 gcagacgacg gaaagcgtgg catgagcgag ggagaaggag ggatcacgag cgacggcggc
     1921 gtcgagatgg cgcgtggctt catcgaggcg agcggcatcg ccgccggaac tgagtcggaa
     1981 accgcgcatg aaatcggcat aggcaagagg atcctcgctg tagcgcgggc gagcctgcgc
     2041 cgttgcgggg ccgagctggc gatcgagagt tgtggctatg tttttggcga tttggtcctg
     2101 ggattcgaac aggcggttga ggtcgagctg gccgttgtgt tcgtaagaaa cgcgttggag
     2161 ttgcgcgtcg aacatttcga cggagagatg ccactggtct ttggcggact gcatggcgcc
     2221 gcggatgaca aatcgcacgt cgaggcgttg ggcaacgtcc gcgggagacg ccgaaggaga
     2281 ttcgggaaga acggtggaag tcggaaggac gtcgaggccg ttgagattgc cgaggcgtgc
     2341 gatcaaggcg tccgcaaaac cgaggccgag accgttgtcg tccgcggaat tgccaaggat
     2401 ggcgagaggc aggacagcga gagaggtgcg cttggactcg gcaagcacgg cgacacgcgg
     2461 ggaggaggaa gaagcgtcgg cagtaagatg gcgcgcgtga atgacgtgga cgggcccgat
     2521 gaagcggtag cccttgccaa tgacggtttc gaggaagcgc ggcttttccg aaacgtcgcc
     2581 gagggctaca cgaatttttc gcacgatgtt gttgacggcg ctttcggtat cgacgaaaag
     2641 gtcggagcgc cagagcttgg cgacgatttc ttcgcgggcg acgagctgtt cgcggcggct
     2701 gaccagaaag agcaagagtt ccatgggctt gcgggcgagt tggacgggac ggccgtggcg
     2761 gcgcaattga taacggccaa ggtcgagctc gatttcagac ggattgctgg cttggttccg
     2821 gttcggctgt tgacgacgca ttg
//
LOCUS       DASTPM010000538         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_162837, whole genome shotgun sequence.
ACCESSION   DASTPM010000538 DASTPM010000000
VERSION     DASTPM010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162837"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1866
                     /locus_tag="VFR42_09545"
     CDS             <1..1866
                     /locus_tag="VFR42_09545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0369442.1"
                     /translation="LELLRVLASEASIAIENARLFQEERTKARHLSLLNTISRNAIAT
                     LNPDEMLAKITEQLEAGLTYDHIGIGQLEYSTREIVIQAEAGKRRGALGERIPLGKGL
                     IGHVARNGQLAAYNASVPADAGFKPLLSDSLAAIALPVFYAEQLHGILYVESSQPADF
                     SEEEVLLLRTLADLIAGALHNALSFQKAQEQAITDGLTGVKTHRFFMEALSAEWKRST
                     RAGRSFALVLMDLDRFKFVNDFYGHLEGDLVLQRVGHILETNCRRSDVVSRYGGDEFV
                     ILMPETNMEHARQLASKLRGWVSADPLLREKNISASFGIACYPLHGSSPQELIQVADA
                     SMYLSKHQGGNAVSTADHFDPNEAKKWKRDVLEAYLGVTLKRLFATGPEAFEEIYSRL
                     KQFTESLAATEVSNGTSTEAPAPDGPQALPQAVLDTVTSLAFAIDAKDHYTQGHSQKV
                     SAYAALIAEALGLNDLEIEEIRLGGVLHDIGKVAIPENILNKNGPLNPDEWDTMKSHV
                     TFGAKILEPLAPLARIREMVEHHHEFFDGSGYPHSLQGENIPLGARIIAVADAYDTIT
                     SDRTYKKARVAAEALAELERCANAQFDAKVVAAFVRTMKVMPNPIIEVASVTRNS"
     gene            complement(1895..2638)
                     /locus_tag="VFR42_09550"
     CDS             complement(1895..2638)
                     /locus_tag="VFR42_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0369443.1"
                     /translation="MKPRTKSPHVSTKTGQLQLDRKNTANQSPSSSGATQGGRPLKPD
                     APDLKEAIRLAQQGDAAAFETIYQMHSRRVYSLCLRMVSDPVEAEDLTQEAFLQLFRK
                     IHTFRGESAFSSWLHRLTANIVLMRFRKKRPVAVSLDELTRPDNEKERPTLELGSPDL
                     RLAGLFDRVNLHAALEQLPHGYKHMFLLHDVHGYEHNEIATMLGCSVGNSKSQLHKAR
                     KRLRELLNEVRHYTSRQVPGMAPASTACD"
BASE COUNT          624 a          741 c          981 g          617 t
ORIGIN      
        1 ctggagttac tgcgtgtgct ggccagcgaa gccagcatcg caatcgaaaa cgcgagactg
       61 ttccaggaag agcgaacaaa ggcgcggcac ctttcgctgc tgaatacgat ttcgcggaat
      121 gcgatcgcaa cgctgaatcc ggacgagatg ctggcgaaga ttacggagca gctcgaggcc
      181 ggcctcacgt acgaccacat cgggatcggg cagctggagt attccacgcg agagatcgtg
      241 atccaggcgg aggcagggaa gcgccgcggt gcgctgggag agcgaattcc gctgggtaag
      301 ggcttgatcg ggcatgtcgc gcgcaatggg cagttggcgg cgtacaacgc gagtgttccc
      361 gcggacgccg ggttcaaacc acttttgtcc gactcgctgg cggccattgc gctgccagtg
      421 ttctatgcgg agcagttgca cgggatctta tacgtggaat cttcgcaacc cgccgatttt
      481 tccgaggaag aagtgctgct gctgcgcacg ttggcggact tgattgccgg cgccctgcac
      541 aacgcgctct cctttcagaa ggcgcaagag caagccatta cggacgggct cacgggcgtc
      601 aagacgcatc gtttcttcat ggaagcattg tcggcggaat ggaagaggtc cacgcgcgcg
      661 gggcggtcgt ttgcgctggt gctgatggac ctggatcgat tcaagtttgt gaatgacttt
      721 tacgggcacc tggaaggcga cctggttttg cagcgggtgg gccatatttt ggagacgaat
      781 tgccggcgct cggatgtggt gtcgcgttac ggcggcgacg agttcgtgat cctgatgccg
      841 gaaacaaata tggaacacgc gcggcaactg gcgtcgaagc tgcgcggctg ggtatcagcc
      901 gatccgctgc tgagggagaa aaacatcagt gcgagctttg gcattgcgtg ttacccgctc
      961 cacggatctt caccgcagga actgattcag gttgcggacg cttccatgta tttgtccaag
     1021 caccagggag ggaatgcggt ctcaaccgcc gatcactttg acccgaatga ggcgaaaaag
     1081 tggaagcggg acgtgttgga agcgtacctc ggggtgacgc tcaagcgcct gttcgccacc
     1141 gggccggagg cgtttgagga aatatactcg cgactgaagc agttcacgga atcgctcgcg
     1201 gccacggaag taagcaatgg gacgtcgacg gaagcgcccg ctccggatgg gccgcaggcg
     1261 ctgccgcaag cggtgctcga cacggtcacg tcgctggcgt tcgcgattga cgcaaaggac
     1321 cattacaccc agggacattc gcaaaaggtg tccgcgtatg ctgcgctgat tgcggaagcg
     1381 cttggattga acgatctgga gatcgaggag atccggctcg gcggggtgct gcacgacatc
     1441 gggaaagtag ccattccgga gaacatctta aacaagaacg gaccgttgaa tccggacgaa
     1501 tgggacacga tgaagtcgca cgtaacgttc ggagcgaaga ttttggagcc gcttgcgccg
     1561 ctggcgcgca tacgggagat ggtggagcat caccatgagt ttttcgatgg ctccggatac
     1621 ccgcactcgc ttcaaggaga aaatatcccg ctgggcgcga gaattatcgc ggtcgcagac
     1681 gcctacgata cgattacgag cgaccgtacc tacaaaaagg cgcgcgttgc cgcggaggcg
     1741 ctggcggaac tggaacgctg cgcgaatgcg cagtttgacg cgaaggtggt ggccgcgttc
     1801 gtgcgaacga tgaaggtcat gcccaatccc attattgagg tcgcgtcggt aactaggaat
     1861 tcctagtcga tagccgtact gagtgcccgg cggtctagtc gcaggccgta ctggcgggag
     1921 ccatccctgg cacctgacgg gaagtatagt ggcgcacttc gttcaaaagt tcgcgtaagc
     1981 gcttgcgagc cttatgtagc tgtgatttgg agtttcctac cgagcatccg agcatggtgg
     2041 cgatttcgtt gtgctcgtat ccgtggacat catggagcag gaacatgtgc ttatatccat
     2101 gcggaagttg ctccaaggca gcgtgaaggt tgacacggtc gaagagaccg gcgaggcgta
     2161 aatctggact gccgagttcc agagtgggcc gctctttttc attatcgggc cgagtcaatt
     2221 cgtcaagcga gacagcgacg ggccgcttct tgcggaaacg catcagcacg atattggcgg
     2281 tgaggcgatg cagccaagaa gagaaggcgg attcaccgcg gaaggtgtga attttgcgaa
     2341 agagctgcaa gaaggcttcc tgcgtcaagt cttccgcttc gactgggtcc gagaccatgc
     2401 gaaggcagag agaatagacg cggcggctgt gcatctggta gatagtttcg aaagcggcag
     2461 catcgccctg ctgagccaaa cgaatagctt ctttgagatc cggtgcatcc ggcttgagcg
     2521 gccttccacc ttgcgttgcg cctgatgagg atggcgattg gttcgcggtg tttttgcggt
     2581 cgagctggag ttgccctgtt ttggtactga cgtgagggct tttggttctc ggtttcatat
     2641 caatctcatg tattttgacg ttcccaatcg ggaaagcttg tcaccgcgcg gtaaacgcga
     2701 tgtcatcatt gtgcggcggc gaaaaaggga tcccttgacc tcgtggacga ggaggtggcg
     2761 gctaatttgc tattcttggt tcgcgaattg gaggattaat gcaaacggca tccgtgaaat
     2821 ggatcgacgg gcaaaggttt gtggcggcag ggccctcggg gcacgcaatt gtggtcgacg
     2881 cggatcgtga aatcaacagc gggccggggc cgatggagtt gttgctgttg gcgttggggg
     2941 cttgcacggc gactgacgtc gta
//
LOCUS       DASTPM010000539         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_767612, whole genome shotgun sequence.
ACCESSION   DASTPM010000539 DASTPM010000000
VERSION     DASTPM010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767612"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="VFR42_09555"
     CDS             complement(<1..1651)
                     /locus_tag="VFR42_09555"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369444.1"
                     /translation="MNHELSLHPLDPSPGSSEPAAAERLAAAQPRTASAWLPLRWQIG
                     ILLGVTLLLYANSLFNGFTMDDELYIQRNPQVTLHSLASLFQPNPASNVFRPLTFATF
                     AMNWIVAGYQPFGYHLVNLLLQAAVVILLLMVLRKLLQGYANLEFICFAAALLFAGHP
                     LHTEAVSSIVGRSELLAAGFLLAAWLFHLQDRYVLALLSFALALLSKESAIGLLPLAL
                     AGDYAKSKLKPWTRYAGIAALTLLYVGALWRIQGGHFGAASVSVLDNPLTLLPAHLRI
                     LNAVRIAWKYAGLLIFPATLSCDYSYDQITLYANWQHLLLPLLGALAVFALWIWSASK
                     RNVIFSLAGAIYFTGFAATSNILTRTGTIFGERLAYFPSAGFCLLAAVCLAQFLTRQR
                     IAALALLGLATTAFGARTIVRNRDWRDNVSLYLAGAAASPNSAKMRTFRGIVYMGQGK
                     WELSRNDLETALRISPDYPDAVEALGLLKVRTGELEAALEYLQKALAISSGADFDYDY
                     RAANLVALQIQMGKLDDAMKLLNRRIPESPNYSRLWSNRAAL"
     gene            complement(1738..>3694)
                     /locus_tag="VFR42_09560"
     CDS             complement(1738..>3694)
                     /locus_tag="VFR42_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0369445.1"
                     /translation="VDFRAREASSIVRVELHPNLNVTKIEISAGKAVSFDRDALNPLL
                     LTVNLPTPVAANTTVSLTFTYSGLLANEENSPVPGVRLASIYKDGAYLLLPARWFPLT
                     AYPTNRYTGTFRLTVPDTFAVAGTGKTSSPTPVAAKSAAEGGRLLYVFECKDAEPNGT
                     FVAGPLQFNAKQAEGLDVNVYAPQDASKNAGDFANAVARSVTAFSDLFGPLPDPNFTV
                     AQMPDGGLREFSGPGLLVMSKRAWDPKINDRVIARLVASQWWGNRVLPATRGDVWISD
                     GLARYSEALYAEQNAGKEAGLRAVDDFAVGALMNEEAAPISQAARLTPFTSDYRSVVM
                     NKGAMQFHMLRAQIGDVAFKSLLHKFYATYAGKQATNAQFEQLAVQQMQAALKPGQEA
                     PNLNGFFAQWLNSTGVPDFKIEYVVYRTPKGFRVVGKISQPLDTFSMPLQLRIETEGN
                     PELKTVDVTGTESTFTVETFGRPKPGGIKIDPNNVVLKGNTGLRARAAIARGEELAES
                     GRYYDAIQQYQKALNIQPGRPLANFRMGEAFFYQKNYQAAANAFRESLQGVPEPSEKW
                     TEVWGHLYLGMIFDLLGQRERAVNEYSKAKQTNDNTGAAQEAADRYLKKPYNEGGAQA
                     ASAAKPTGEAAPPAADDGRPHLKKPNP"
BASE COUNT          788 a         1035 c         1136 g          735 t
ORIGIN      
        1 gcagcgctgc gcgattcgac cacaagcgag aatagttagg cgattccggg atccgccggt
       61 tcaacagctt catggcgtcg tcgagctttc ccatctgaat ttgaagcgcg accaggttcg
      121 cggcgcgata gtcgtagtca aaatccgcgc cagagctgat ggccagcgcc ttttgcagat
      181 attcgagagc ggcctccaat tccccggtac gcaccttcaa taaacccaat gcttcaaccg
      241 cgtcaggata gtccgggctg atgcgaagcg ctgtctcaag gtcgttgcga gagagttccc
      301 attttccctg tcccatgtaa acaattccgc gaaaggtgcg catcttggcg ctgttgggcg
      361 aggcggcagc gccggccagg tagagtgaca cgttatcgcg ccaatcgcgg ttgcgaacga
      421 tcgtgcgagc accgaaagcg gttgtagcca ggccgagaag tgcgagcgca gcaatacgct
      481 ggcgagtcaa gaattgcgcc aggcatacgg cggcgagcaa gcagaatccg gcagacggaa
      541 agtaggctag gcgctcgccg aaaatggtgc cggtgcgggt aagtatgttg gatgtcgccg
      601 cgaatcccgt gaagtagatt gcgccggcca gggaaaaaat gacattacgc ttcgaggcgc
      661 tccaaatcca gagagcaaag accgctaatg ctcccagcaa tggaagaagc agatgctgcc
      721 agttggcgta cagcgtgatt tggtcgtacg agtaatcaca cgaaagcgtt gcgggaaaaa
      781 tcagcaagcc ggcatatttc caggcaatgc gaactgcgtt caagatgcgc aagtgtgctg
      841 gcaacaaggt aagtggattg tccagcacgg aaacgctggc agcgccgaaa tgcccgccct
      901 gaattctcca gagcgcaccg acgtatagca gcgtaagtgc tgcgatgccg gcgtagcgcg
      961 tccaaggctt cagtttgctc ttcgcgtagt cgcccgccag cgccaatgga agcaagccga
     1021 tggcggactc cttggaaagc agggcgagcg cgaaacttag cagcgccagt acgtagcgat
     1081 cctgcaggtg gaagagccat gccgccagca aaaatccagc agctagcaat tccgaacggc
     1141 ctacgatcga actgacggcc tctgtgtgca gtggatgccc ggcaaaaagc agggcggcgg
     1201 caaagcagat gaattcaaga tttgcgtagc cttgcagcaa cttgcgcaga accatgagca
     1261 ataggatgac gaccgccgcc tgcaacagca gatttaccag gtgatagcca aacggctggt
     1321 atccggccac gatccagttc atcgcgaacg ttgcgaaggt cagcgggcgg aacacgttgc
     1381 ttgccgggtt gggctgaaaa agcgatgcca gcgaatgtag cgtgacctgc gggttgcgct
     1441 ggatgtacag ctcatcatcc atggtgaatc cgttaaagag cgagttggcg taaagcaaca
     1501 gcgtgacgcc gagcagtatg ccgatctgcc agcgaagagg cagccatgcc gaggcggtac
     1561 gcggctgggc cgccgccaat ctttccgctg cggcgggttc gctcgaacca ggggagggat
     1621 cgagagggtg caaactcaat tcatggttca cttcacgagc atacgtgatc ggttagagtt
     1681 cggagcggac gatcacggag caggtgacgt gaggaaaaac cgccctgacg gtcgcaacta
     1741 tgggttcggc tttttcaagt ggggccggcc gtcgtcggcc gctggaggag cggcttcgcc
     1801 cgtcggtttg gcggcggacg ctgcttgcgc cccgccttcg ttgtagggct ttttcagata
     1861 acgatcggcg gcttcctggg cggcgcccgt attgtcgttg gtctgcttgg ctttgctgta
     1921 ttcgttgacg gcgcgttcac gctggcccag caaatcgaaa atcattccga gatagagatg
     1981 cccccacact tccgtccatt tctcggatgg ttcgggcacg ccttgcagag attcccggaa
     2041 ggcgttcgcc gcggcctgat agtttttctg atagaagaac gcttcaccca tgcggaaatt
     2101 tgccagcggg cgccccggct gaatgttcaa tgccttttga tactgctgaa tcgcgtcgta
     2161 gtagcggccg gattcggcga gttcttcgcc gcgcgcgatg gcggcgcggg cgcgtaatcc
     2221 ggtgttgccc ttgagaacga cattgttcgg atcgatcttg atgccgcccg gcttgggccg
     2281 gccaaacgtc tcgacggtga aggtggattc cgttccggtc acgtcgacag ttttcaattc
     2341 cggattgcct tcggtttcga tgcgcagctg gagcggcatc gagaatgtgt ccagcggctg
     2401 actgattttg cctacgacgc ggaagccctt cggcgtgcgg tacaccacgt attcgatctt
     2461 gaagtccggc acaccggtgg aattcagcca ctgtgcaaaa aaaccgttga ggttgggggc
     2521 ttcctggccg ggtttcaatg cggcctgcat ctgctgcacg gccagctgtt caaattgcgc
     2581 gttggtcgcc tgtttgcccg cgtacgttgc gtagaatttg tgcaacagcg acttgaacgc
     2641 cacgtcgcca atctgcgcgc gcagcatatg aaattgcatc gcgcccttgt tcatcaccac
     2701 ggagcggtag tcgctggtga acggggtcaa ccgcgccgcc tgcgaaatcg gagcagcttc
     2761 ttcattcatc aaggcgccga ctgcgaaatc gtccaccgcg cgcaggccgg cctccttgcc
     2821 ggcattctgc tcggcgtaga gcgcttcgga ataacgcgcc agcccatcgc tgatccacac
     2881 gtcgccgcgc gtcgccggca acacccggtt accccaccac tgcgaagcca ccagccgcgc
     2941 aatcacgcga tcattaattt tgggatccca agcgcgcttc gacatcacca gcaggcccgg
     3001 cccggagaac tcacgtagtc caccgtccgg catctgcgcg acggtgaagt ttggatcggg
     3061 caatggcccg aacagatcgg agaaagcggt gacggaacgc gctacggcat tggcgaagtc
     3121 gccagcgttt ttcgacgcat cttgaggcgc ataaacgttc acgtccaacc cttcggcttg
     3181 tttcgcgttg aactgaaggg gaccggcaac gaatgtgcca ttcggttcgg cgtccttgca
     3241 ttcaaacacg tacagtaagc gaccgccctc tgctgcagac tttgccgcga ctggcgtagg
     3301 cgatgacgtt ttaccggtgc ctgccacggc gaaggtatct ggaaccgtca ggcgaaatgt
     3361 tccggtatag cgattggtcg gatacgcagt gagcggaaac cagcgcgctg gcagcagcaa
     3421 atacgcgccg tctttataaa tcgaagccag acggacgcca ggcacggggc tgttctcttc
     3481 attggccagc aagccggaat acgtgaacgt cagcgaaacg gtggtgttcg cagcaaccgg
     3541 tgtgggcagg ttgacggtca gcaggagcgg gtttagcgca tcgcggtcaa agctcaccgc
     3601 tttgcccgca gagatctcaa ttttggtcac gttcagattg gggtgcagtt cgacccgaac
     3661 gatgctggag gcttcacggg cgcgaaaatc cact
//
LOCUS       DASTPM010000540         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1374215, whole genome shotgun sequence.
ACCESSION   DASTPM010000540 DASTPM010000000
VERSION     DASTPM010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374215"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1909)
                     /locus_tag="VFR42_09565"
     CDS             complement(107..1909)
                     /locus_tag="VFR42_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0369446.1"
                     /translation="MAPPRTYAWIAAVSLSVLGVYAILSLTLPRGESLNAFGNIMQCL
                     VPLLANAGLLLNAGTPHWRRNVFWMLTAMSCTLWMIGQFQWTYYEVYLHRSLPDLYPG
                     DIIFFLRGVPLMAALALRPHLKRGEMQLRFGYLDFVLLLSWWVFLYVFVVLPWMYAEP
                     SLGQYNFTYDVISNVQNMVTLIWFGALWLQSRRAWRTVYAHLFGADVMYMLSSQVINV
                     GISLKEYHTGSLYDLPLMASFLWFAAAGFIAYKNRDLLDDATVSPTESEQRKSESTVA
                     SRLALAAVVSLPAFAIYTLKFSHDGAAVKEFRLLTVLVAAVPLGALVFLRQHLADSDR
                     ARLLARAEQSVENLQRLQQQMVQTEKLVSLGQLAAGAAHEINNPLTAILGYSDLLADD
                     ASLPEKTRGVAAKIRDQARRTKTLVGNLLSFARQVPAERTLLDLNTVVNNAVQLRALD
                     LKHSGAKIELKLESVLPGVRGDGNQLMQVFFNVINNAVDAMDTHSGGGTLTIKTLRDR
                     ANVVILFSDTGPGIKEPSRVFDPFYTTKPIGKGTGLGLSICFGIVQEHGGKILCYNGQ
                     EGGAVFRVELPAVLAALPTRDALVAGSSPVAKNS"
     gene            complement(2058..>2623)
                     /locus_tag="VFR42_09570"
     CDS             complement(2058..>2623)
                     /locus_tag="VFR42_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369447.1"
                     /translation="LVRPAQEDVADEQKEERMAQFLWPQAVQKFHELQGNVGQIFVAY
                     RDEKKRLRQTEIPALLTDSAYLQEFQTRLGPRWLGETPDRRHVDKKLHANPGFFKTIF
                     VLVALLGIVAAIAAVGLLGFLGTILNFMSIQKMLLDLQDRNFVSFGYRAASYVALFFM
                     GYLLQRVIRAKLDAMKRPLPSRPPFRP"
BASE COUNT          593 a          756 c          752 g          522 t
ORIGIN      
        1 cattttgctt gtctggattc gcctgcggaa gggaatgaat tagctgaccg tatggtctgt
       61 ttcgcggtca gtagcgctgc tttcctcata caacggtaaa ttaatctcaa gagttctttg
      121 ctacaggcga gctgccggca actagcgcgt cgcgcgtggg cagcgcggcc agcaccgcgg
      181 gcagttccac gcggaacacc gcgccccctt cctggccgtt gtagcagaga atttttccac
      241 cgtgctcctg cacgattcca aagcaaatac tgagccccag ccctgtgcct tttccaatcg
      301 gtttggtggt atagaacgga tcgaagacgc gagacggctc cttgatcccc gggcccgtat
      361 cggaaaacaa aatgaccaca ttggcgcggt cgcgcaatgt tttaatggtc agcgttccac
      421 cgccgctgtg cgtgtccatc gcatccaccg cattgttgat aacgttgaaa aacacctgca
      481 tcagctggtt gccgtcgccg cgtactcccg gcaaaaccga ttccagcttt aattcgattt
      541 tcgcaccgga atgtttcaaa tccagtgcgc gcaactgcac cgcgttgttc accaccgtat
      601 ttaagtccag cagcgtccgc tccgccggaa cttgccgcgc aaaactcagc agatttccca
      661 ccaatgtttt agtgcggcgc gcctgatcgc ggattttggc cgcaacgccg cgcgtttttt
      721 ccggcagcga cgcatcatcc gccagcaaat ccgaatagcc gagaatcgcc gtgagcggat
      781 tgttgatttc gtgcgccgcg cccgccgcca gttgccccag cgaaaccaat ttttccgttt
      841 gtaccatttg ctgctgcaaa cgctggagat tctccacgga ctgttccgcc cgcgccagca
      901 gccgcgcgcg atcgctgtcc gccagatgtt gtcgcaaaaa taccagcgca cccagcggta
      961 ctgccgccac cagcaccgtc agcaaacgga attccttcac cgcggcgccg tcatgcgaaa
     1021 acttcagagt gtaaatcgca aaagccggaa gcgaaactac tgctgccagc gccagccgcg
     1081 atgcgaccgt gctctcgctc tttctctgct cgctttctgt gggcgatacc gtcgcatcat
     1141 ccagcaaatc gcgattcttg tacgcgataa atcctgccgc cgcgaaccac aggaacgagg
     1201 ccatcagcgg caaatcatac aaactgcccg tatgatattc cttcaggctg atgccaacat
     1261 tgatgacctg cgacgacagc atgtacatca catccgcgcc gaagagatga gcataaacgg
     1321 tgcgccacgc acgccggctc tgcagccaca gcgcgccgaa ccagatcagc gtgaccatgt
     1381 tttgcacgtt gctgatcacg tcgtaggtga agttgtactg gccgagcgaa ggctccgcat
     1441 acatccaggg cagcaccaca aacacgtata ggaaaaccca ccaggagagc agcagcacaa
     1501 aatccagata gccgaaacgc agctgcattt cgccgcgttt cagatgcggg cgcagcgcca
     1561 gcgccgccat cagcggcacg ccgcgcagga aaaagataat gtcgccgggg tacaaatccg
     1621 gcagcgaccg gtgcagataa acttcgtagt aagtccactg gaattgcccg atcatccaca
     1681 gcgtgcagga catggcggtg agcatccaga aaacgttgcg gcgccagtgg ggggtgccgg
     1741 cgttcaggag cagcccagca ttcgccagca gcggcaccag gcactgcatg atgttgccga
     1801 aagcattcag ggactcaccc cggggcaggg tgagcgagag aattgcatag acgccgagga
     1861 cagagaggga cactgcggcg atccacgcat atgtgcgggg aggggccatg agctaaatca
     1921 attttatgtt gccgtgagcg gcgccgtatc ggcgttctga cgcgcgaatg gccaaagcat
     1981 tactgaggac ttaagcggtg attactgaac ggaactctga atgccggctg cgcggaagag
     2041 gtctcggtag cacgcggcta aggccggaaa ggcggccgcg aagggagagg gcgcttcatg
     2101 gcgtcgagct tggcgcggat gacgcgttgc aacaagtagc ccatgaagaa gagcgcgacg
     2161 tagctggcgg cgcgatagcc aaagctgacg aagtttctgt cctgcaggtc gagaagcatt
     2221 ttttggatgg acatgaaatt caggatggtg ccgagaaatc cgagtaggcc gacagcggcg
     2281 atcgctgcga cgatacccag caaggcaacg aggacaaaaa ttgttttgaa aaagccggga
     2341 ttggcgtgca gctttttgtc gacgtgccgg cgatctggtg tctcgccgag ccagcggggg
     2401 ccgaggcggg tttgaaattc ctgcagataa gccgaatccg tgagcagtgc gggaatttcc
     2461 gtctgccgga gccgtttttt ttcgtcgcgg taagccacaa aaatctggcc gacgttgcct
     2521 tgcagttcgt gaaatttctg aacggcctga ggccaaagga attgggccat gcgctcttct
     2581 ttttgttcgt cggcaacgtc ttcttgtgca ggacggacca gag
//
LOCUS       DASTPM010000541         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1374250, whole genome shotgun sequence.
ACCESSION   DASTPM010000541 DASTPM010000000
VERSION     DASTPM010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374250"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            564..1349
                     /locus_tag="VFR42_09575"
     CDS             564..1349
                     /locus_tag="VFR42_09575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0369448.1"
                     /translation="MIRVQNLVKKFADFSAVNDISFEVAKGEIFAFLGPNGAGKTTTI
                     KMLTTLLPPTSGCLQLDGLDPARQPNEVRQQFGIVFQDPSLDGDLTAAENLELHGVLY
                     HVPRKIRRERAEMLLKLFELWERKDTQVKKFSGGMKRRLEIVRGFLHTPKILFLDEPT
                     LGLDPQSRNQLWTHVKRINETEQVTVFLTTHYMEEADRVAQRIAVIDHGRIVAMGSPP
                     ELKQRTNTESLEEAFLALTGTTIRDETANSSDQLRQMVQMWRR"
     gene            1351..2097
                     /locus_tag="VFR42_09580"
     CDS             1351..2097
                     /locus_tag="VFR42_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369449.1"
                     /translation="MHAIFILWLREVKKYSRSRAQIVASLGQPLLYLLVLGFGLGPVF
                     QKSGNGSYLQFVAPGVIGMTVLFTSVFSGIALLFDRQFGFLKETLVAPVPRIRIMIGR
                     TLGGATVAMLQGTLIFAVCLIAGFRPVSLAAIPLGFVFMALIAIVFAALGTAIGSGLQ
                     DMQGFQLIMNFLVLPIFFLSGALFPLTNIPTALKFVTRLDPLSYGVDGLRGALSGLTQ
                     FGFATNLITLAVIAVALLILGAWRFSKIEI"
     gene            2099..2725
                     /locus_tag="VFR42_09585"
     CDS             2099..2725
                     /locus_tag="VFR42_09585"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0369450.1"
                     /translation="MARGLSQRAHQKVLEAALDLFAERGIDATSVDSIAAASGVSKAT
                     IYKHWADKDALCMEVLVYIHELDKGPPDLDSGDLKADLIAYLVYEPAPRKAELQKRIM
                     PHLIAYSARNQEFGRAWRTRVLERARTGLKKLLCLGMDRGIFPAILDEELAVPLLLGP
                     MLFSHILGPRLDRKWLAEGTVNSFWKAHARVEPQPPASKSLRRLRRRG"
     gene            2773..3585
                     /locus_tag="VFR42_09590"
     CDS             2773..3585
                     /locus_tag="VFR42_09590"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0369451.1"
                     /translation="MSNHYLEVAIETALQAGQILREEYERPPDIAYKGDVDLVTQADK
                     RSERAIVARLSKYFPEHSISAEEGTGHVRSSEFRWHVDPLDGTTNFAHKYPCFSVSIA
                     LAQNDSVLAGVVYNPIYNELFTAARGEGATLNGKKISVSHVSTLATSLLCTGFPVHKR
                     RSSQNIHYYYDFTLRSHGVRRDGSAALDLASVAAGRFDGFWEFGLNPWDTAAGILLVH
                     EAGGTISDFDGKPYVVGGPVVLATNSRIHEEMRQVALTISQRAPDAPLEPPP"
BASE COUNT          779 a         1076 c         1088 g          840 t
ORIGIN      
        1 atacatttac tggaacccgc aggcgggctt cccgcgcccc gtgtcataaa aggcttattc
       61 cccttgcgct tgaactgttt tttgctttac cgagcctagc cagaaggcca ctcgctccgg
      121 aaaccacgaa gacgcgctgc gcccctaaag cgcgccagca cggaggaaac tcctgcagga
      181 gcatgcgaaa cggattcacc ggatttggct agtaaatcgg cagcctacga cgaggtgatg
      241 gggttccaat gaaaactgcc agcctgtcct ttccggtcgg aggcgaggtg gccttcagtg
      301 cgacaacgga aaaaatggtt cgtcgcgccg tgcacgcgac ggcaagtcca aaccttcgcg
      361 tgcaaaatct gcagcctcgc aaaaacgcca cacgaatacc cggcactgaa gcgatcatgt
      421 gccagcttcg agtgctctaa ttcatctgcc gctagggcga catagtcgct tattgaaacc
      481 ggcgcgcctt tcttctttag cttgacataa ctaaactgaa ccgtttagta tgtcttcctg
      541 cgccgacttt cccgaggttg accatgatcc gagtgcaaaa tctcgtcaaa aaattcgccg
      601 atttttccgc cgtgaacgac atctcttttg aggtggctaa gggcgaaatc tttgcttttc
      661 tcggacctaa tggcgcgggg aaaaccacta ccatcaagat gctgaccacg ctgctgccgc
      721 ccactagcgg ttgcctgcaa ctggacgggc tggaccccgc gcggcagccc aacgaagttc
      781 gccagcagtt tggcatcgtc ttccaggacc ccagtctgga tggggacctg accgccgccg
      841 agaacctgga gctgcacgga gtgttgtacc acgttccgcg aaaaatccgc cgcgagcgcg
      901 cggaaatgct gctgaaacta tttgaactgt gggagcgcaa ggacacgcag gtaaaaaagt
      961 tttctggcgg gatgaaacgg cgactggaaa tcgtgcgcgg ctttctgcat acgccgaaaa
     1021 ttctgttcct ggatgagccg acgctgggcc tggatccgca aagccgcaac cagttgtgga
     1081 cccacgtgaa acgcatcaac gaaaccgagc aagtgaccgt gtttctgacc acccactata
     1141 tggaagaagc ggaccgcgtg gcgcaacgca tcgccgtcat tgaccacggc cgcattgtgg
     1201 ccatgggctc gccgccagag ctgaaacaac gcaccaacac ggaatctctg gaagaagcgt
     1261 tcctggcact gaccggcacc accatccgcg atgaaaccgc aaattcttcc gatcaactgc
     1321 gccagatggt gcagatgtgg aggcgatagc atgcacgcaa tttttatttt gtggttgcgc
     1381 gaagtaaaaa agtattcgcg atcgcgcgcg cagattgtgg cttcgctcgg ccagccgctg
     1441 ctctacctgt tggtcttggg gttcgggctg ggcccagtgt ttcagaaatc gggcaacggc
     1501 agctatctgc agttcgtcgc gccgggcgtg atcggcatga ccgtactttt tacttcggtc
     1561 ttttcgggaa tcgcactgct ctttgaccgt cagtttggct ttttgaaaga gactctcgtt
     1621 gctcccgtgc cgcgtatccg aatcatgatt ggccgcacgc ttggcggtgc aacagttgcg
     1681 atgttgcagg gcacactgat ttttgcggtt tgcctgattg cgggatttcg tccggtaagc
     1741 ctggctgcga tcccgctggg attcgtgttc atggcgctga tcgccatcgt gtttgccgcg
     1801 ctggggacgg caatcggctc gggcttgcag gacatgcagg gttttcagct catcatgaat
     1861 tttctggtgc tgccgatttt cttcttgtcg ggcgcgctgt tcccgctgac aaatattccc
     1921 acggcgctga aatttgtcac gcggcttgat ccgctgtcgt acggggtgga tggattgcgc
     1981 ggagcgttaa gcgggctgac ccagttcgga ttcgcgacga atctgatcac gcttgccgtc
     2041 attgcggtgg cgttgttgat cctgggagcc tggcgatttt cgaagatcga gatctgacat
     2101 ggcacgcggc ctcagccagc gcgcacatca aaaggttctg gaagcggcgc tcgacctttt
     2161 cgcagagcgt ggcattgacg ccaccagcgt cgattcgatc gccgcagcat ctggcgtcag
     2221 caaagccacc atctacaaac actgggcgga caaagacgcg ttgtgcatgg aagtgctggt
     2281 atatatccat gaactagaca agggcccgcc ggacctcgat tccggcgatc tcaaagctga
     2341 tctgattgcg tacctggtgt acgagccggc tccgcgcaag gctgaactcc agaagcggat
     2401 catgccgcat ttaattgcgt actctgcgcg taatcaggaa tttggccgcg cgtggcggac
     2461 acgcgtgctg gagcgagcgc gcacgggatt gaaaaaattg ctctgccttg gaatggaccg
     2521 cggcattttt ccggcaattc ttgatgaaga attagccgtg ccgctgctgc tgggccccat
     2581 gctctttagc catatcctgg ggccgcgcct ggatcgcaag tggctggccg aaggcacggt
     2641 gaattctttt tggaaggccc acgcgcgagt cgaacctcaa cctccggcat ccaaatcgct
     2701 gcggcgtttg cggcgccgcg gttaacagaa aattccagac ctgataccgt agaagctccg
     2761 gcatcggaga ccatgtccaa ccactatctc gaagttgcca ttgaaactgc attgcaggcg
     2821 ggccagattc ttcgcgaaga gtatgagcgc cctccggaca ttgcctacaa gggcgacgtg
     2881 gatctggtga cgcaggcgga caagcgcagt gaacgcgcga ttgtggcgcg cctttccaaa
     2941 tattttccgg aacactcgat ttccgcagaa gaaggcacgg gccacgtacg cagttcggag
     3001 tttcgctggc acgtcgatcc cctcgacggg accacgaatt ttgcgcacaa gtatccgtgc
     3061 ttttcagtgt cgatcgcgct tgcacaaaac gattcggtgc tggccggcgt ggtctacaat
     3121 ccgatttaca acgaactgtt caccgcggcg cgcggcgaag gcgcgacgct gaacggaaag
     3181 aaaatttcgg tgtcgcacgt ttcgaccctc gccacgagtc ttttgtgtac gggatttccg
     3241 gtacacaagc gtcgctccag ccaaaatatt cactactact atgatttcac cttgcgctcg
     3301 catggagtgc gccgcgacgg ttccgccgct ttggatttgg ccagcgtggc cgccggacgc
     3361 ttcgacggat tctgggagtt cggcctgaat ccttgggaca ccgcagcggg aatcttgctg
     3421 gtgcacgaag caggtggcac tatctccgat ttcgatggca agccttatgt agtcggtggc
     3481 ccagtggtgc tggccacgaa ctcgcgcatt cacgaagaaa tgcgccaggt ggcgctcacg
     3541 atttcgcagc gcgctccgga cgctccgctc gaaccgccgc cctgagcggc tttccgagac
     3601 ctggcgattc ggaatctatt cttcgagtta actcatcttg ggcagggtat gactcacgtg
     3661 gtgcgggcgc ggtccggcgc ggaccaaccc gcttctactt ggcgccccat tccttataga
     3721 gcttggtgat cgttggcccg atggcaaggc tccaggcgaa aatcgccacg ttcttgaact
     3781 ttt
//
LOCUS       DASTPM010000542         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_652224, whole genome shotgun sequence.
ACCESSION   DASTPM010000542 DASTPM010000000
VERSION     DASTPM010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652224"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFR42_09595"
     CDS             complement(<1..418)
                     /locus_tag="VFR42_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369452.1"
                     /translation="MAGATAHYCSEKDAREKKVVSFAAAAIAAALLLSCAIGLTAAAP
                     RPPDTLEQLQEKFDKETDGVRKAKQVQKLGDAQFAKEREAAKAGDFVTAGVVMEKYRD
                     NVRAALEALKKTRPDAEKHPNGYKQLEFHTGQGLREV"
     gene            complement(361..1731)
                     /locus_tag="VFR42_09600"
     CDS             complement(361..1731)
                     /locus_tag="VFR42_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369453.1"
                     /translation="MIYRDSRAETEAGIIPALRHRVLRGLSVPAAAFLALTILVTPCH
                     GQAPGAGKGTKQRVANPLNELLDEAKRDIDAQKFEAAIAPLQKFIAEKEDFAYAHFQL
                     GYVYTALGRGKEARPEYERALQQDPKMSEAAVNLGILLLNDEPAAALGPLQKAVELLP
                     TQSRPRTLLGLAYEKSGDLKNAVTAYEGALALDPKDSETSLHLAQLLFRQGRAVEAET
                     RFRAVLAGEPESRAALLGLAQSLEAEKKPEAVEAYQIYLKGQPDDAGARQRLVHLLIL
                     NEKFDEALAEMERTQKGGPPSLESLKLRADILIGQKKWDEAVVVLKQAVALAPQDAQL
                     RGGLGRTYMQQRDFANAEKELRAAIGLDARNLVYVKDMTSTHYLAGDYPGALSLLDEV
                     AKREKPTPGELFIRALCYDKLQQIKAALEAYQNFLAADQDRNPDQVWQAQQRIIVLKK
                     MLERKR"
     gene            complement(1728..>4015)
                     /gene="uvrA"
                     /locus_tag="VFR42_09605"
     CDS             complement(1728..>4015)
                     /gene="uvrA"
                     /locus_tag="VFR42_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU0369454.1"
                     /translation="LIDSVEICYREGTGEAIVEFVAEANGATPFAAEPRAQDTAERLV
                     FNERFECKNDGTIYQEPEPRLFSFNNPYGACPRCQGFGNTIDFDLNLVVPDPSKSLDE
                     GAIEPWTKPRFRSLLNEAKAWAKGQGIATSVPWRQLKEEDRQKLLEGDPKNDYDGVKG
                     FFAWLERKKYKLHVRVFLSRYRGYATCPECSGTRLRPEARAVRVAERTITDVCKLTVK
                     EARKFFSETRLSEAQMKVADKILEEIRSRLQFLDEVGLDYLSLDRLTSTLSGGESQRI
                     QLATSLGSHLVGALYVLDEPSIGLHPRDTKRLIDILKSLRDLGNTVLVVEHDPDTIEA
                     ADRVVDLGPGAGELGGKLLFAGSYQEILRDAKSITGKYLSGELRIPVPNTRHKPAGKF
                     LRLYGATLHNLKNVDAMIPLGVLTVVTGVSGSGKSTFVHDVIYKALLAKRNGGTEKEF
                     CDRLDGDYQVKDVVIVDQSPIGRTPRSNPATYLKAFDAIREVFADTPDAKRRGYTAGH
                     FSFNVPGGRCETCQGDGTVTVEMQFLADVELICEECRGTRFKTSVLEVQYHGKNVHDV
                     LQMTVREALAFFVNVPKVTSKLRVLNEIGLGYLRLGQSGTTLSGGEAQRLKLAAHLTR
                     TENQGVLYILDEPTTGLHFDDIAKLLAAFRKLLESGASLLVIEHNLDVIKSADWLIDM
                     GPEGGDMGGKIIATGTPEQVARNSQSHTGKFLAKVLQANGQGRNGEPAHQSGQAKRNG
                     NSASNGIEVTRDGQGPISPAK"
BASE COUNT          765 a         1439 c          904 g          907 t
ORIGIN      
        1 gcacttcccg cagcccttgc ccggtatgaa attccagttg cttgtagccg ttcgggtgtt
       61 tctccgcatc cggacgcgtt ttcttcagcg cctccagcgc tgcccgcaca ttgtcgcggt
      121 atttttccat taccacgccc gcggtcacaa agtccccggc cttggcggcc tcgcgttcct
      181 tcgcgaattg cgcatcgccc agtttctgaa cctgcttggc tttgcgtacg ccatctgttt
      241 ccttgtcgaa tttttcctgc aattgctcga gcgtatccgg cgggcgcggt gcggctgccg
      301 tcagaccaat agcgcacgac agcagtaacg cggctgcgat ggccgcagcc gcaaaggaaa
      361 ctaccttttt ctctcgagca tctttttcag aacaataatg cgctgttgcg cctgccatac
      421 ctggtccggg ttgcggtcct gatcggccgc cagaaaattc tggtacgcct ccagcgccgc
      481 cttgatctgc tgcaatttat cgtagcagag tgcgcgaata aacaactcgc ccggcgtcgg
      541 cttttcccgc ttcgcaactt catctagcaa cgacagcgca ccgggatagt cgccggccag
      601 atagtgcgtg ctggtcatat ctttcacgta gaccaggttc cttgcgtcca gcccaatcgc
      661 cgcccgcaat tccttctccg cattcgcgaa gtcgcgctgc tgcatatacg tgcggcccag
      721 cccgccgcgc aactgcgcat cctgcggcgc cagcgccacc gcctgtttca gcacaaccac
      781 cgcttcgtcc cattttttct gcccgatcaa aatgtccgcg cgcagcttca acgattccag
      841 cgacggcggt ccgcctttct gcgtcctctc catctcggcc agcgcttcgt cgaatttctc
      901 attcaagatc agcaggtgca ccaaccgctg ccgggcccct gcatcgtccg gctgcccttt
      961 caaatagatt tggtatgcct ccaccgcttc cggttttttc tctgcctcaa gactttgcgc
     1021 cagccccagc aaggccgcgc gcgactccgg ttccccagcc agtaccgcgc gaaatctcgt
     1081 ttccgcttcc acggcgcggc cttggcggaa aagcagttgc gccaggtgca aactggtctc
     1141 gctgtctttc ggatccagcg ccagtgctcc ttcatacgcg gtgaccgcat ttttcaagtc
     1201 gccggatttt tcatacgcca gccccagcag cgtgcgcggc cggctctgcg tcggcagcaa
     1261 ttccaccgct ttttgcagcg gccctagcgc cgcggctggc tcatcattca acaacagaat
     1321 ccccagattc accgccgctt ccgacatttt cggatcctgc tgcagcgccc gctcatattc
     1381 cggccgcgcc tctttgccgc gccccagcgc tgtatacaca tagcccagct gaaaatgggc
     1441 gtacgcaaaa tcctctttct ctgcaatgaa tttctgcaac ggcgcaatcg ccgcttcaaa
     1501 cttctgcgca tcaatatcgc gcttcgcctc gtccagcaat tcattcagcg gatttgccac
     1561 tcgctgcttg gttcccttgc ctgcacccgg cgcctgccca tggcacggag tcaccaaaat
     1621 cgtaagcgcc aaaaaagccg cagccggcac tgacaaaccc cgtagcacac gatggcgtag
     1681 cgcggggata atgccggctt ccgtctcagc tcgtgaatcg cgataaatca tttcgcagga
     1741 gaaatcgggc cttgcccgtc gcgagttact tctatcccat tcgatgcgct gttcccgttc
     1801 cgctttgcct gcccgctctg gtgggccggt tcgccgttgc ggccctgccc gttcgcctgc
     1861 agcaccttgg ccaaaaactt tcccgtatgc gactgtgaat tccgcgccac ctgctccggc
     1921 gttcctgtag caataatctt cccgcccatg tcgccgccct ccggccccat atctatcaac
     1981 cagtccgcac tcttgatcac atccaggttg tgctcaatca ccagcagcga cgcgcctgat
     2041 tccagcaatt tgcgaaacgc tgccagcagt ttcgcaatat cgtcgaagtg caggcccgtc
     2101 gtcggctcat ccaaaatgta cagcacgccc tgattttccg tccgcgtcag atgcgccgcc
     2161 aatttcaatc gctgcgcctc gccccctgac aacgtcgtcc ccgactgccc cagccgcaaa
     2221 tatcccaaac caatttcatt cagcaccctc agcttcgacg tcaccttcgg cacattcaca
     2281 aaaaacgcca gcgcctcccg caccgtcatc tgcagcacgt catgcacatt tttcccgtgg
     2341 tactgcactt ccagcacgct ggtcttgaat cgcgtcccgc ggcactcttc gcaaatcaat
     2401 tccacgtccg ccaaaaactg catttccacg gtcaccgtgc cgtcgccctg gcacgtttcg
     2461 catctcccgc ctggcacgtt gaacgaaaaa tgtcccgccg tatatccgcg ccgcttggca
     2521 tccggcgtat ccgcaaacac ttcgcgaatc gcatcaaacg ccttcaaata cgtcgcagga
     2581 ttcgaacgcg gcgtcctccc gatcggcgac tggtccacaa tcactacatc tttcacctgg
     2641 tagtcgccgt ccaaccggtc gcaaaactcc ttctctgttc cgccattccg cttggccagc
     2701 agcgctttat aaatcacgtc atgcacaaac gtcgattttc ccgatccgct cacgcccgtc
     2761 accaccgtca gcactcccag cggaatcatc gcatccacat ttttcaagtt gtgcaacgtc
     2821 gcgccataca gccgcaaaaa tttccccgcc ggcttgtgcc gcgtattcgg caccggaatc
     2881 cgcagttccc cgctcaaata ttttccggta atcgacttcg catctctcag aatttcctgg
     2941 tagcttcccg caaacaacaa ttttccgcca agctcgcccg ctcccggccc caagtcaacc
     3001 acccggtccg ctgcttcgat cgtgtccgga tcgtgctcca ccaccagcac cgtattcccc
     3061 agatcgcgca gtgatttcaa aatatcgatc agccgcttgg tatcccgcgg atgtaatcca
     3121 atcgacggtt catccagcac atacagcgcg cccaccagat gcgaacccag cgatgtcgcc
     3181 agctgaatcc gctgcgactc cccgcccgac agtgtcgatg tcagccggtc cagcgacaaa
     3241 taatccaaac ctacttcatc caaaaactgc agccgcgaac gaatctcttc caaaatcttg
     3301 tccgccacct tcatctgcgc ttcgctcagc ctcgtctccg aaaaaaactt tcgcgcctct
     3361 ttcaccgtca gcttgcagac gtccgttatc gtgcgctccg ccacccgcac cgcccgggct
     3421 tccggacgca ggcgcgtccc gctgcattca gggcacgtcg cgtacccacg ataccggctc
     3481 aaaaacaccc gcacgtgtag cttgtatttc ttgcgttcca gccaggcaaa aaaccctttc
     3541 acgccgtcgt agtcgttttt cggatcgcct tccagcaatt tctgccggtc ctcttctttc
     3601 agttgccgcc acggcacact cgtcgcaatt ccctgtccct tggcccacgc cttcgcctca
     3661 ttcagcagcg agcgaaatcg cggcttggtc cacggctcaa tcgcgccctc gtccagcgat
     3721 ttgctcggat ccggcaccac caaattcaaa tcaaaatcaa tcgtgttccc gaatccctgg
     3781 cagcgcgggc acgcgccata tggattgttg aacgaaaaca gccgcggctc gggctcctga
     3841 taaatcgttc catcgttctt gcattcaaac cgctcattaa aaaccaaccg ctcagctgta
     3901 tcctgagccc gcggttccgc ggcaaagggc gtggcgccat tcgcctcagc gacaaattcc
     3961 acgatcgctt cgcccgtgcc ttcgcgataa cagatctcca ccgaatcgat cagcc
//
LOCUS       DASTPM010000543         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_422809, whole genome shotgun sequence.
ACCESSION   DASTPM010000543 DASTPM010000000
VERSION     DASTPM010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422809"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /gene="thpR"
                     /locus_tag="VFR42_09610"
     CDS             <1..341
                     /gene="thpR"
                     /locus_tag="VFR42_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU0369455.1"
                     /translation="GFFPNARRPTVVWAGLRALQELAALAGKIVTALAVCGVSPETRP
                     FAPHLTLARLKDTRLSDALRAQIERAQNRSFGKQIVRDFHLIESKLKSTGAEYTTLRS
                     FPFAGEGINQ"
     gene            338..970
                     /gene="plsY"
                     /locus_tag="VFR42_09615"
     CDS             338..970
                     /gene="plsY"
                     /locus_tag="VFR42_09615"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU0369456.1"
                     /translation="MTVPLTPLIVYLVSYVLGSIPFGILMARLFGGRDVRKAGSGNIG
                     ATNVARVVGPLAGILTLLLDAAKGWFAVWIAARTMHGEAFAMALAGFFAMLGHCFPLW
                     LRFRGGKGVATAAGVFGALCPAAMLAAVILFLLVVGFWRFVSLGSIAAAAAIPLLVYL
                     LWAPQFAPPVSLTLVSLAIAALVVYQHRGNISRLARGEEPKFSFSKKDPA"
     gene            967..2007
                     /locus_tag="VFR42_09620"
     CDS             967..2007
                     /locus_tag="VFR42_09620"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU0369457.1"
                     /translation="MKKIAILGAGSWGTALAIVLARSRQPHHISMWVHDPELAEFLRR
                     ERVNSTYLPDHRLAEAIEVTNHLAEAIDGAQMVVGVMPSAFARKVYTAARPFWQHDTT
                     IVSATKGLEPRTHARMSQIVLDVLGEDAALRTAVLSGPSFAAEVARGEPTAVVVAGRA
                     VLELTRVSGRHYSDSAAEAQAEFAGPNFRLYTNSDVLGVELAGAMKNVIAIAAGVCSG
                     LRLGSNSLAALITRGLAEMSRLATKLGAKPETLSGLAGLGDLVLTCTGALSRNRHVGV
                     QLGKGRAIAEILAEMKMVAEGVETTAALMDLAAEVQIELPITEQVHAILHEGKPPAEA
                     IRDVMERPAKRE"
     gene            2152..>3166
                     /locus_tag="VFR42_09625"
     CDS             2152..>3166
                     /locus_tag="VFR42_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEU0369458.1"
                     /translation="MSANFRMLFDRVPIGRVRILWLVLGAMLLVSAPPLLLYHLQVLK
                     LSQDKLSDNERMQQSELTRSLAQELQQFDANVTQQLISERQILVLTGLIQDVEDPSAE
                     PKVTRLLENFVESNRTTFLYMTAVGKSGKGTSASQGNFRAEKDPFVSKALQRAFVTCM
                     QSPQLGSVKFRSDPLAIEPDNQPAFVIAIPLNVEQQFTGMIAAVVSLDPILNRLKDSS
                     VRGRSVFVVDHQGHIVAHPDTARFTPGEDVSASSSVVSRVKTLPQELRNTETMRFTET
                     ENKRSVEMIGTYSTFPEVNWAVIAQRSLAQARTDAGVNELNRQALTFVSVVVVIAILV
                     GYLF"
BASE COUNT          589 a         1051 c          857 g          669 t
ORIGIN      
        1 tcggcttttt tccaaatgcg cgccgtccaa ccgtagtctg ggctggtttg cgagccctcc
       61 aggagctcgc cgccctagct ggcaaaatcg tcaccgctct cgccgtctgc ggcgtttcgc
      121 cggagacgcg tccgttcgct ccgcatttga cgctggcccg cctcaaggac actcgcctat
      181 cggacgctct gcgcgctcaa atcgaacgcg cccaaaaccg ctcgttcggc aaacaaatcg
      241 ttcgggactt tcacttgatc gaaagcaagc tcaaatccac cggcgcggag tacactacgc
      301 tgcgctcgtt tccgtttgcc ggcgaaggaa tcaaccagtg accgttccgc tgactccgct
      361 cattgtctac ttggtgtcgt acgtcctcgg ctccattccg ttcggcattc tcatggccag
      421 gctttttggc ggacgcgacg ttcgcaaagc cggcagcgga aacatcgggg ccaccaacgt
      481 cgcgcgggtg gtcggtcctc tcgccggaat tctgacgctg ctcctcgatg cggccaaagg
      541 ttggttcgcg gtttggattg ccgcgcgcac catgcacggc gaagctttcg caatggccct
      601 cgccggcttt ttcgccatgc tcggccattg ctttccgctc tggctgcgtt ttcgcggcgg
      661 caaaggagtc gccactgccg caggcgtatt tggcgcactc tgccctgccg cgatgcttgc
      721 tgcggtgatt ctctttcttc tggtagtggg tttctggcgc ttcgtttccc tcggttcgat
      781 cgccgcggcc gcggccatcc cgcttctggt ctatttgtta tgggcgcccc agttcgctcc
      841 gccggtaagc ctcactctgg tctcgctcgc tatcgcggcc ctggtcgtct atcaacatcg
      901 cggcaatatc tcccgcctgg ctcgcggcga ggaaccaaag ttttccttca gcaaaaagga
      961 cccggcgtga aaaaaatcgc gattctgggc gcaggtagct ggggaactgc cctggccatc
     1021 gtcctcgcgc gcagccgcca gccgcaccac atctccatgt gggtgcacga tcctgagctt
     1081 gccgaatttt tgcgccggga gcgcgtgaat tccacttatc tgccggacca tcgactcgcg
     1141 gaagcgattg aagttaccaa ccacctcgcc gaggcgattg acggtgcgca gatggtcgtt
     1201 ggcgtcatgc cttctgcatt cgcgcgcaaa gtctacaccg ccgctcgccc gttctggcaa
     1261 cacgacacca ccatcgtcag cgcaaccaaa ggcctcgaac cgcgcacgca cgcccgcatg
     1321 agtcaaatcg tcctcgatgt tcttggcgaa gacgcggccc tgcgcaccgc cgttttgtcc
     1381 ggaccgtcct ttgccgccga agtggcgcgc ggcgaaccta ccgcggtcgt tgtagcaggc
     1441 cgtgctgttc tggagttaac acgcgtctcc ggccgccact actccgactc cgccgccgaa
     1501 gcccaagccg agttcgccgg ccccaacttt cgcctttaca ccaactccga cgttctcggc
     1561 gttgaacttg ccggcgcgat gaaaaacgtg atcgccattg cggcgggcgt ctgcagcgga
     1621 ctgagattgg gctccaactc gctcgcagcg ctcatcaccc gtggactcgc ggaaatgtcc
     1681 cgcctcgcca ccaaattggg cgctaaaccc gaaacgctca gcggcctcgc agggctaggc
     1741 gacctggtgc tcacttgcac cggggcactg agccgcaacc gccacgtcgg cgttcaactg
     1801 ggcaaaggac gcgcgattgc cgaaatcctc gccgagatga aaatggttgc ggagggcgtg
     1861 gagaccactg ccgcactgat ggacctcgcc gccgaagtgc aaatcgagct gcccatcaca
     1921 gagcaagtcc acgctatcct ccatgaaggc aagccaccgg ccgaagccat tcgtgatgtg
     1981 atggaacgcc cggccaaacg cgaataaacc tcttttgctt cgccgattag agacttctcg
     2041 ccgcgcacct gtcccttcgg acgggtacgc aagaattaat gcggtatcgg cactttttcc
     2101 tgcagggtca ggttttctcg cacctccttt tcctaaaata tcagcaggaa tatgtctgcg
     2161 aactttcgca tgctcttcga ccgcgtcccc attgggcgcg tgcgtattct ttggctggtg
     2221 ctgggcgcca tgctgctcgt cagcgctccg ccgctcctgc tgtatcacct ccaggtgctg
     2281 aaactcagcc aggacaagct atccgacaac gaacgcatgc agcaatccga attgacccgt
     2341 tccctggcgc aggaacttca gcagtttgac gccaatgtta ctcagcaatt gatcagcgaa
     2401 cgccagatcc tggtgctcac cggcctgatt caggacgtcg aagaccccag cgccgagccc
     2461 aaagtcacgc ggctcctgga aaatttcgtc gaaagtaacc gcaccacgtt tctgtatatg
     2521 actgctgtag gtaaatccgg caagggtacc agcgcatcgc agggaaattt tcgcgcggaa
     2581 aaagatccct tcgtcagcaa agcccttcaa cgcgccttcg tcacctgcat gcagtcgccg
     2641 caactcggct cagtgaagtt tcgcagcgat ccactggcca tcgaacccga caatcagccg
     2701 gcctttgtta tcgccatccc tctgaacgtg gagcagcagt tcaccggcat gatcgccgcc
     2761 gttgtttcgc tcgatcccat tctcaaccgg ctaaaagatt ccagcgtgcg cggccgcagc
     2821 gtgttcgtcg tggaccatca gggccacatc gttgcgcatc ccgataccgc ccgtttcact
     2881 cccggtgaag acgtcagcgc cagttcttcc gtggtctctc gcgtaaagac gcttccccag
     2941 gaactccgca ataccgaaac catgcgcttc acggaaaccg aaaacaaacg ctcggtggag
     3001 atgatcggca cctacagcac ctttccggaa gtcaattggg ccgtgatcgc gcagcgcagc
     3061 ctggcccagg cacgcaccga tgccggcgtg aacgaattga accgtcaggc tttgacgttc
     3121 gtcagcgtcg tcgttgtgat cgccatcctc gtcggctatc tcttcg
//
LOCUS       DASTPM010000544         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_622598, whole genome shotgun sequence.
ACCESSION   DASTPM010000544 DASTPM010000000
VERSION     DASTPM010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622598"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..618
                     /locus_tag="VFR42_09630"
     CDS             286..618
                     /locus_tag="VFR42_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0369459.1"
                     /translation="MSATERRSTQRFTMRLPLTVRWTTGAAVGETSTESRDVSSRGVY
                     FYLGKDVKEGSPVEILLTLPNEITLAGPVRVRCLGRVQRTESRDETSVGVVAAIERYE
                     FLRGDEER"
     gene            complement(880..1116)
                     /locus_tag="VFR42_09635"
     CDS             complement(880..1116)
                     /locus_tag="VFR42_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369460.1"
                     /translation="MVNIGSQINTLAIGGGRIQNQIKDINATTRNVFFDRRFAHNGFA
                     PPWYPSTTITSSGSHSAAMTTTIQRTKWFNRTTY"
     gene            complement(1162..1506)
                     /locus_tag="VFR42_09640"
     CDS             complement(1162..1506)
                     /locus_tag="VFR42_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369461.1"
                     /translation="MVAGVPTQNDPATGAVTRDATMLYVNGNITALSGPGQGVAAIRD
                     GTALTITAENNVTVTGDILYHKEPVTTTQNQIPGSPADTLIPGNDRGQVVGIFTSTGD
                     IQLKHSQSNGNL"
     gene            1726..>2628
                     /locus_tag="VFR42_09645"
     CDS             1726..>2628
                     /locus_tag="VFR42_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0369462.1"
                     /translation="MPVAHRRLQILGGTSMPDSPAQQTIVLSKAEYGSTTERMATPEE
                     LLALQLLAEEEDAVMQAIESVAQAPEPKPVQPVETVPTTKVEAGSPQIAATLSQIELP
                     QTPNDVSAVETAKEVAQPQDVVADRVALSHRVSLPAGVFELAAILDQHRIWVESGGEA
                     GSKADLCGANLENADLTGANLQGAFLQRADLRGADLSMANLRHASLVQADLSNANLLG
                     TEFRGANLMGATLYGAEGLWLGRLGGANLYDAMLPETISSIDGAKTVWETTKSARWFY
                     FLMLTVCTLCGLGVATTSDARLILD"
BASE COUNT          497 a          742 c          788 g          601 t
ORIGIN      
        1 tgggacaatt ttcggccagc cgtgccttac acaaaggtac aggggacgaa gtgcaggtac
       61 acccgtcgct gcaatctcgg gcacctcagg aagcaccgtt gtttctgtgg attgcagccc
      121 ttggacgacg gaccggcgcg cccttgggtc gtgtcccctc gatggaatct gaaatttact
      181 agtaccttcg taccgccttc ttccgaaacg attttgtacc tgttgtccta ttgttccgta
      241 gtcaggctcc ggctacactc tctgcagatt tgaggaggtt ccgtcttgtc ggccactgaa
      301 cgccgcagca cccaacgttt taccatgcgg ctgccgctta ccgtccgctg gaccaccgga
      361 gcggctgttg gggaaaccag cacggaatcc cgcgacgtca gctcgcgcgg ggtttacttc
      421 tacctgggga aagacgtcaa ggaaggctcg cccgttgaaa tcctgctgac gcttcccaat
      481 gagattacgc ttgccggtcc ggttcgcgtt cgctgcctgg gacgcgtgca acgcacggaa
      541 agccgcgacg agacctccgt cggagtggta gccgcgattg aacgttacga atttctgcgc
      601 ggcgacgaag agcggtaacc tgtttctctg taggctcttg caaaagtttc ttgctcgctc
      661 actgcggagg cgggcaggat aaagccgggg tgccttggcc cctcagcgaa gcgccccggc
      721 atttttctcc cctgccgttg cttaacccca aaatcgaaat tgcaactttg cgtttcacgg
      781 cacgccgaac tcgtttttct tcattccgct gtgcgcaaaa aatagagcgg ccagcgagaa
      841 ctcgctgccg ctccgtgtgc gctaggcgga aaaactcggt tagtacgtcg tgcgattaaa
      901 ccacttggtg cgttgaatgg tagtggtcat ggcggcggaa tgggagccgc tgctggtgat
      961 cgtagtggac ggataccagg gcggcgcaaa tccgttgtgc gcgaagcgcc ggtcgaagaa
     1021 gacgttacgc gtggtggcgt tgatgtcttt gatctgattt tgaatgcggc cgccgccgat
     1081 ggcgagggta ttgatctgac tgccgatgtt gaccaagcct ccgctgccgc cattggagat
     1141 ggtggccggc gaagcgtcaa tttacaggtt cccgttggac tggctgtgct tgagttgaat
     1201 gtcgccggtg ctcgtgaaaa tgccgaccac ctggccacgg tcgttgccgg gtatcaaggt
     1261 gtccgccggc gagcctggaa tctggttctg cgtggtggtg acgggttcct tgtggtagag
     1321 gatgtcgccg gtcacggtga cattgttttc cgccgtgatg gtcagcgccg tgccgtcccg
     1381 aatggcggcg acgccctggc ccggtccaga gagtgcagtg atgttgccat tgacatacag
     1441 catggtggcg tcgcgagtta ccgcgccggt ggcgggatcg ttttgtgtgg ggactccggc
     1501 aaccaaagtt ctacaaaaag ttttccctgt gcgctgctgc tcctaaccgt tcctgtttcc
     1561 ttgcgtttgg cagttgaggc aacaaatttc tctttctcat ccatgagaag gctaccggtt
     1621 cttcatgcct gtcaccgggg acaatgttgt tttttcgccg cctgaccttt tgggcaggcg
     1681 actttttctt cccatttcgg caatcatgtc ggtgctgaga gattcgtgcc ggttgcccat
     1741 cggcgcctgc aaattctggg gggaacgtcc atgccggatt caccggcgca gcagactatc
     1801 gtcttgagca aggcggagta cggctctact acggagcgca tggctacgcc cgaagagctt
     1861 cttgcgttgc agctcctggc ggaagaagaa gacgccgtta tgcaggccat tgagtcggtc
     1921 gcgcaagcgc ccgaaccaaa gcccgtccag cctgtggaaa ccgtaccaac caccaaggtt
     1981 gaagccgggt ctccgcaaat tgccgctact ctttcgcaga tcgagctgcc gcaaacgccc
     2041 aacgacgtca gcgcggtcga aacggcaaaa gaggtcgcac agccacaaga cgtcgttgct
     2101 gacagagttg cgttgtcaca ccgggtgagc ctaccagccg gtgtgttcga actcgctgcc
     2161 atcctcgatc aacatcgtat ttgggtagag agtggaggcg aggcagggtc caaagccgac
     2221 ctatgcgggg ccaatctgga aaacgccgat ctcaccggcg ccaatctgca aggagcattc
     2281 ctgcaacggg cggatctgcg cggagcggac ctgtccatgg ccaacttgcg ccacgcttcg
     2341 ttggtgcaag cggacttgag caacgccaac ctgctcggca ctgaatttcg cggcgccaac
     2401 ctgatgggcg caaccctata tggtgctgaa ggcttgtggc tgggccgtct cggtggcgca
     2461 aatctttatg acgctatgct tccagaaacg atctcttcca tcgacggcgc caaaactgtt
     2521 tgggaaacca ccaagagcgc tcgctggttc tatttcttga tgctcacggt gtgcacgctt
     2581 tgtggattgg gcgtggctac caccagcgac gctcgtttga ttctcgac
//
LOCUS       DASTPM010000545         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_509130, whole genome shotgun sequence.
ACCESSION   DASTPM010000545 DASTPM010000000
VERSION     DASTPM010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509130"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFR42_09650"
     CDS             <1..186
                     /locus_tag="VFR42_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369463.1"
                     /translation="LNLAGEYADQVVLLQKGKALRVGPPASVYQRDLLEQVFQTQLTV
                     ETGASGRPRVSILPGAQ"
     regulatory      290..511
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            579..2909
                     /locus_tag="VFR42_09655"
     CDS             579..2909
                     /locus_tag="VFR42_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0369464.1"
                     /translation="MKTLPNAGFVKPTLKPCLLGVLASLIFFSFTAVLSGQSASPDTQ
                     LKGTLLDSSGAGVGGVQVVAQLANDPQSHLWKATSTTDGAYTLTLPPGNYHVVFQRKP
                     FAARQFDLALTTPGETRTLDLRLDLERLSSSVVVTAQPQPLPIQQTTAPVDVITRGDI
                     DARLAAILTDVLPYSPGIAIGRTGPEGGSASIFLNGGNSAYTKVLVDGTPVNESGNAV
                     DFANFTLDNIDKVEVVRGAESAVYGSDAVSGVIQVFTHRGTTRIPEVLIFAEGGNFDT
                     GRGGAQISGLLGNFDYSGAASYFSTSGPQDLNTFLNRTLSGNFGYRLGEENQLRLTLR
                     NNTSNAQTPGQTLLEPANPDAHTDYHFFSSNARWDFATGKHWHHQLIGAESYNRQFTA
                     NPVQSFFIEGQNSFCPQTNPAAVATAEFCDFTDSGSHYQYNRASLNAQSSYLLENFAA
                     TAGYQYEVENASIPFLAQGHVRRNNQAGFLDFRYSPHPRASLNFGARAEANGYFGTRV
                     VPRAGASLALLEGRGFWGETRLRAFYGQGVKEPRFDQLFDDQFGDLGNPNLKPESSKT
                     WTVGLDQKLADNRVRLTGEYFSSRFYNMISFAFCDLSNNFCDLPPGTGPLFGFGYFFN
                     TDRSRARGTNITAEARVNPWLRVRGNYTYDDSLVIAAPNVFDPAQLPGNRLIRRPVNS
                     GSLTAFANWRRLGVVLSGYFSGQRTDSDFLGLGFNHTPGYARFDLATTYQFARGISAY
                     LRAANLFDKSYQDALGYPALGRELRIGMNYRFGGKM"
BASE COUNT          569 a         1053 c          721 g          598 t
ORIGIN      
        1 ctcaacctcg ctggtgagta cgccgaccag gtcgtgttgc tgcaaaaggg caaggccctg
       61 cgtgtcggtc cgcccgccag cgtctaccag cgcgatttgc tcgaacaggt tttccagaca
      121 caactcaccg tggagaccgg cgcatccggc cgcccccgcg tgagcatcct gcctggagcg
      181 cagtaaatgt tgtcattcca agcgcggatt ttgcgccagg aatctgcttc tcacggccgc
      241 gccgattttg ctgcggctct ctagcaaccc acagcgggcg atgcccctta acaacaatcg
      301 cgggcctcgt gcacgggaag acggagctct ttctagcggc gggtttattc cgctgcctcc
      361 gcaagagcca gccgtcgccg ccgcgcgact gtaaagcgga attcgcaagc cgccactgct
      421 tgaagtgctt ccaagggaat gcacgctcgc gggaaggcgc ttgcagcacc gccgccaagc
      481 caggagaccg gcgaggtccg cagtcccgtt tcgccaattc atcggtacga aatgggaaca
      541 gccttcaact cccgcgcgtg agggagcgga ggagaccaat gaaaactttg cctaacgcag
      601 ggtttgtaaa accaaccctg aaaccctgtc tgcttggcgt tctcgcttct ctgattttct
      661 tcagcttcac cgcagtcttg tcaggacaat ccgcttcacc tgatactcaa ctcaaaggca
      721 ctctcctcga ctccagcggc gccggtgtcg gcggagtcca ggtcgtcgcg caactcgcca
      781 acgatccgca gtcgcacctc tggaaagcca ccagcaccac cgacggcgcc tacacactga
      841 cgcttccgcc gggcaactac cacgttgttt tccagcgcaa accgtttgcc gcgcgccaat
      901 tcgaccttgc tctcaccacc ccgggcgaaa cgcgcacact cgatctgcgc ctcgatctcg
      961 agcgcctctc ttccagcgtc gtcgtcaccg cgcagcctca gccactgcca attcagcaaa
     1021 ccactgcgcc cgtggacgta atcacccgcg gggacattga tgcgcgcctc gccgccatcc
     1081 tcacggacgt gctgccgtat tcacccggca tcgccattgg ccgcaccgga cccgaaggcg
     1141 gctccgcttc catatttctc aacggcggca actccgccta taccaaagtc ctcgtcgatg
     1201 gcactcccgt caacgaatct ggcaacgctg tggacttcgc caacttcacg ctcgacaaca
     1261 tcgacaaagt tgaagtcgtc cgcggcgccg aatccgccgt ctatggctcc gatgccgtat
     1321 ccggtgtcat tcaggtattc actcatcgcg gcaccacgcg cattcccgaa gttctcattt
     1381 ttgccgaagg cggaaacttc gacaccggtc gcggcggcgc gcaaatcagc ggcttgctcg
     1441 gcaatttcga ttactccgga gcagcctcct acttctccac cagcggcccg caggacttga
     1501 acactttcct caaccgcacc ctctccggca atttcggcta ccgtcttggt gaagaaaacc
     1561 agctccgcct cacgctgcgc aacaacacca gcaacgcgca aacgcccgga caaactctgc
     1621 tcgaacctgc caaccccgat gcccacaccg actaccattt cttcagctcc aatgcccgct
     1681 gggatttcgc caccggcaag cactggcatc accagctcat aggcgccgaa tcctacaacc
     1741 gccagttcac cgccaatccc gtccagagct tctttatcga gggacaaaat tcgttctgtc
     1801 cgcaaaccaa tccggcggcc gtcgctaccg ccgaattctg cgacttcacc gattccggca
     1861 gccactacca atacaaccgc gccagcctca acgcgcagtc cagctacctt ctggaaaatt
     1921 tcgccgccac cgctggctat cagtacgaag tcgaaaacgc ctctattccg tttctggcgc
     1981 agggccacgt tcgtcgcaac aatcaagccg gtttcctgga ctttcgctac tcgccgcatc
     2041 cgcgtgcttc gctgaacttc ggcgcacgcg ccgaagccaa cggatatttc ggcacacgcg
     2101 ttgtccctcg cgctggcgct tcgctcgctc ttctcgaagg ccgcggtttc tggggcgaaa
     2161 ctcgcctccg cgcgttctac ggtcagggcg tcaaggagcc gcgcttcgat cagcttttcg
     2221 atgatcagtt cggcgacctc ggcaatccca acctcaaacc cgaatccagc aagacctgga
     2281 ccgtcggcct tgaccagaaa ctcgccgaca atcgcgtccg tctcaccggt gaatattttt
     2341 ccagccgctt ctacaacatg atcagcttcg ctttttgcga tctctccaac aatttctgcg
     2401 accttccgcc gggcaccggg cccctttttg gcttcggata tttctttaac accgaccgtt
     2461 ctcgcgcccg cgggacgaac atcaccgcag aagcgcgcgt caatccctgg cttcgcgtcc
     2521 gcggtaacta cacgtatgac gatagcttgg tgattgcagc ccccaacgtt ttcgatccgg
     2581 cgcaactccc cggcaatcgt ctgattcgtc gtcccgtcaa ttccggctcg ctcaccgctt
     2641 tcgccaactg gcgccgcttg ggtgtagttc tttccggcta tttttccggc cagcgcaccg
     2701 acagcgactt tctcggcctc ggattcaacc acactcccgg atacgcccgc ttcgacttgg
     2761 cgaccacgta tcaattcgcc cgcggcattt ccgcctatct ccgcgcggcc aatctcttcg
     2821 acaagtccta tcaggacgcc ctcggctacc ccgctctcgg tcgcgaactc cgtatcggca
     2881 tgaactaccg cttcggcgga aaaatgtagc agccgcctgc cgtgtaggga cgcggcatgc
     2941 c
//
LOCUS       DASTPM010000546         3571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_105548, whole genome shotgun sequence.
ACCESSION   DASTPM010000546 DASTPM010000000
VERSION     DASTPM010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105548"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1025)
                     /locus_tag="VFR42_09660"
     CDS             complement(366..1025)
                     /locus_tag="VFR42_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369465.1"
                     /translation="MKRIAWLAGIGMVLAALGALAVAQEASLGDYARQARKQKGHPSP
                     SVKKYDNDNLPMNDKVSVVGQAPEAADNTAPAGGGEPAPAADNPTAPSSDANAADKAA
                     NADEKKPSEEKKSASDEQAEKQKMYKDWQHRIHEKQNQIDMLTREVDVANREYRLRAA
                     AFYADAGNRLRNAGAWDKEDAQYKDQMADRQKKLDDAKQQLEDLQEQARRAGVPSSMR
                     E"
     gene            complement(1074..1937)
                     /locus_tag="VFR42_09665"
     CDS             complement(1074..1937)
                     /locus_tag="VFR42_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369466.1"
                     /translation="MKLLTNPIVLRMALLFFASCFAVVMGWLMMRRVRRSLSEGDSLP
                     SVAETAAQLPMYTYNAVIQELKQQKHELATMRDAESRRARSSESLSAAVLSNLSSGVL
                     FFGPNGLARQANPAAKEILGIASPAGMSPAEIFRMASHSSLTGTITLAESVSAVLHTG
                     GSVQRAEVEYLTPSGDHRVLEVTASRVPAADGSLLGMACLINNRTEMTDIQRQVELRG
                     ELSAEMALALRTSLITISGYAQQLAKNRDPELATQLAADIATEAKHLDHTIGGFLAES
                     KKSQAASAAHS"
     gene            complement(1934..2860)
                     /locus_tag="VFR42_09670"
     CDS             complement(1934..2860)
                     /locus_tag="VFR42_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU0369467.1"
                     /translation="MDPLLASASVVFGLIFGSFLNVCIYRLPRDLSVMNPRWSVCPQC
                     KRQIAAYDNIPVLSWLLLRGRCRQCKAPISPRYVLIEVMTAVLFLACTWYFGATIAAL
                     KYCALAFLLLGLIFTDAETKLLPDRMTLSGLALGITFSLFVPVNDLAAQFLPGVVSLP
                     VSSDVSWRLLSLADSLLGALVGASFIYGAGAIYLRARGVEGMGFGDVKLMAMIGAFLG
                     VKLTVFTIFTASIAGSLFGVGTILLVWIKRTRRFVRRQHVTVREARRRAWQSAQTMYR
                     HYEMPFGVFLGSMAMIALFFGKQFMDWYWRLL"
     gene            complement(3055..>3571)
                     /locus_tag="VFR42_09675"
     CDS             complement(3055..>3571)
                     /locus_tag="VFR42_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0369468.1"
                     /translation="IAQQTLGFVREVSEAKALNVSATLNEVVQLYSAKLLTNQIQVQK
                     DFCETCEIRGFAGELRQLFANLLVNAADAMPKGGRLRLRVTRTREYSGLGRTGVRVTF
                     ADNGNGIPAENQHRLFEPFYTTKKDSGTGLGLWLSEGIVRKHHGKIRVRSSARPGNSG
                     TTFSLFLPEHA"
BASE COUNT          758 a         1091 c          975 g          747 t
ORIGIN      
        1 agcagcaggc tggccccgcc gatcacaaca aacagcaatt tcttccatga accgctagcg
       61 aatcgcatga caaccctcct aaagagtcga gtgctgatgc gtgcaagccc ggggcttgcg
      121 gcaccgtgct ggttgttgga agacgacgag gtcaggaggt gatacggaac aacggggcgg
      181 agcccgcaaa cgtagttcgc accatccctc tgctacgttg caggcaggat gcgattacta
      241 tgagagacgc gcgtggcgaa gtccaatcaa aaatatcaat gcgcgccatc gcgcgcgact
      301 tttcataaga cgttcataaa aaatcccggc gcgaacgccg ggatttccaa aattctcaga
      361 tctagttact cgcgcatgga cgacggaacg cccgcgcgcc gggcctgctc ctgcaaatct
      421 tcgagttgtt gcttggcatc gtcaagtttc ttctgcctgt ccgccatctg atccttgtac
      481 tgcgcatctt ccttgtccca ggcgccggca ttacgtaaac ggttgcccgc atcggcgtaa
      541 aatgctgctg cccgcagacg gtactcgcgg ttggcgacat cgacttcgcg cgtcagcatg
      601 tcgatctggt tctgcttttc gtggatcctg tgctgccagt ccttgtacat cttctgcttc
      661 tcggcttgct cgtcgctggc cgatttcttt tcctcagagg gcttcttctc atcggcattc
      721 gcggctttgt ccgccgcatt ggcgtcgctc gaaggcgcag tcgggttgtc ggcagcgggc
      781 gccggttcgc cgccgcctgc gggtgctgtg ttatctgcgg cttccggagc ctggccgaca
      841 accgatactt tgtcattcat cggaagattg tcgttgtcgt acttcttcac agagggcgac
      901 ggatgtccct tttgtttgcg ggcctgacgt gcatagtcac ccaacgacgc ttcttgggcg
      961 acggcgagcg cgcccagcgc cgccagaacc attccgatgc ctgccaacca tgcgatgcgc
     1021 ttcatatgca ttgtcctcac atcttcgcgc cggcagagcc gccgcgagaa tccttaagaa
     1081 tgtgccgccg aagcggcttg cgatttcttg ctttccgcca gaaagccacc aattgtgtga
     1141 tcgagatgct tggcttcggt ggcgatgtcg gccgccagtt gcgtggcgag ttccggatcg
     1201 cgattcttcg ccaactgctg tgcgtatccc gaaatcgtga tcaatgacgt tcggagagcc
     1261 aaagccattt ctgccgacag ttcgccgcgc aactctacct gccgctgaat atctgtcatc
     1321 tccgtgcggt tgttgatcag gcaggccatt ccaagcagcg agccatctgc cgcgggcacg
     1381 cgcgaggctg tgacttccag aacgcggtga tcgcccgagg gcgtgaggta ctcgacctct
     1441 gcccgctgca ccgacccccc agtgtgcagt acagccgaaa cgctctccgc caacgtaatc
     1501 gtgcctgtta aagaagagtg gctcgccatg cgaaaaatct ctgccggact catgccggct
     1561 ggcgaagcga tgcccagaat ttctttggcc gccgggttcg cctgtcgtgc cagcccgttt
     1621 ggtccgaaga agagaacacc actggaaagg ttcgatagca ccgcggcact caagctttcc
     1681 gaactgcggg cgcgccggct ttcggcgtct cgcatcgtgg cgagttcatg tttctgctgc
     1741 ttgagttcct gaatcacagc attgtaggtg tacatgggaa gctgggccgc ggtctctgcg
     1801 acgctcggca gagagtctcc ctcgctcaag cttcttcgca cacgccgcat catcaaccag
     1861 cccatcacaa ccgcgaaaca cgaagcgaag aacagcaatg ccatcctcag cactatggga
     1921 ttggtcagca gcttcacagc agcctccagt accaatccat gaattgcttt ccgaagaaca
     1981 gcgcgatcat tgccatgctg cccagaaaca ctccaaaagg catctcgtag tggcggtaca
     2041 tggtttgcgc ggattgccac gcccgacggc gggcctcacg caccgttaca tgttgacgac
     2101 gaacgaagcg ccgcgtccgc ttgatccaaa ccagcagaat tgttcctaca ccaaatagtg
     2161 accccgctat cgatgccgtg aagatcgtaa acaccgtcag ctttacgccg agaaacgcac
     2221 cgatcatcgc catcagcttc acatcgccga accccatccc ctccacgccg cgtgcccgca
     2281 agtaaatcgc gcccgctccg taaatgaacg aggctccaac taacgcacct agcagagaat
     2341 cggccagcga aagcagtcgc cacgaaacat cggaactcac aggcaacgaa actacgccgg
     2401 gcagaaactg cgccgccaga tcgtttacgg gaacaaacag gctgaaggtg atgcccaagg
     2461 ctagcccgct gagcgtcatc cggtccggca gcaacttcgt ctcggcatcg gtgaagatca
     2521 gccccaacag gaggaaggcg agcgcgcaat acttaagcgc cgctatcgtc gctccgaaat
     2581 accatgtgca cgccaggaac aacaccgcgg tcatcacctc gatcagcaca tatcgcggag
     2641 aaatcggagc tttacattgc cggcaacggc cccgcaaaag aagccagctg aggaccggaa
     2701 tattgtcgta agcagcgatt tgccgcttgc actgcgggca taccgaccag cgcggattca
     2761 tcaccgacaa gtctctcggt aggcgataga tacacacgtt caaaaagctg ccgaagatca
     2821 gcccgaagac cacgctcgcc gatgccagta aaggatccac gctctaggaa cgccttcgtt
     2881 gtgacagttg tgcatcgatt atacggtcgc ggctttgccc tcctttcagt actgaagtca
     2941 ctgtccttct ggacagcgct cctgtaacga acacattcga agggagttgt cgcttaggtg
     3001 actgccgtga tgaccgtggt gcgcacccag ggaaaggaaa accggtcccg acgctcaggc
     3061 gtgctcagga aggaacagcg agaaggtggt tccactgttt ccaggccgcg ccgaactgcg
     3121 aacgcgaatt ttaccgtggt gcttgcgaac gatgccttcc gatagccata gccccagtcc
     3181 ggttcccgag tcttttttcg tcgtgtagaa gggttcaaac agccggtgct ggttttcggc
     3241 agggatacca ttcccgttat cggcaaaggt aacccgcacg cccgtgcgcc ccaatccgga
     3301 gtattcccgc gtccgcgtta cccgcaaacg caatcgcccg cccttgggca tggcatcggc
     3361 cgcgttcacg agtagattgg cgaacagctg gcggagttcg ccggcgaatc cgcgaatctc
     3421 gcacgtctcg caaaagtctt tttgcacttg aatctgattg gtgaggagct tggcggagta
     3481 gagttgcact acttcgttga gagtggccga gacgttcagc gctttcgctt ccgacacttc
     3541 gcgaacaaat cccagggttt gctgtgcaat a
//
LOCUS       DASTPM010000547         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_105564, whole genome shotgun sequence.
ACCESSION   DASTPM010000547 DASTPM010000000
VERSION     DASTPM010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105564"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFR42_09680"
     CDS             complement(<1..423)
                     /locus_tag="VFR42_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0369469.1"
                     /translation="MTETKQSEPWLRGTLGDVPAVARAVLHALQLAEEDLEKFCGALS
                     DAQLNARPDGVAPVAFQIRHIARSVDRLLTYAEGRQLSEAQLEQLRRELEPGANAAKI
                     FAELKEQLRRAEQRIRALAATEDLEAARAVGRRALPTTV"
     gene            502..2373
                     /locus_tag="VFR42_09685"
     CDS             502..2373
                     /locus_tag="VFR42_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU0369470.1"
                     /translation="MLNLIAITGALRRPAIRASLLAWVAAASAIFLAPQWLLAQSAPS
                     TASADLTRYLDDVKALSSPEMEGRGDGTKGLTLAEHLIVERYKSLGLEPAGSKGYLQS
                     FRVVTGRKIQGGTKMSGHANGGKIETYAVHEDFIPLNFSSTGNARASVVFAGYGITAP
                     EFGYDDFHDADVRDKIVLVLRGEPESISEKAPSAGHTTHASLITKAINAKNHGAAALI
                     VVNGKLSHGDSDELRPFGDAEGPEEAGILCLQVKTPVADEWLRPTGLTSAELQDSIEK
                     EKNSGLSIPLDSTVDVTLKIETLRATVNNVLAYLPGKSDEYVIVGAHYDHLGKGDSHS
                     LAPSSIGQIHPGADDNASGTAGVLELARIVAPLAGKLDRGILFANFAGEELGLLGSAE
                     WVKEPTRPLDKAVAMLNMDMIGRIKDDKVFIGGIGTGTTLKSAVEDAANGSSLHFEYS
                     EGGYSSSDHTSFVTKKIPVLFFFSGLHSDYHKPSDTWDKINAASATQLLNLVSKVTVE
                     LAEAAEKPKFVAVVENPHVGGVSSGGGYGPYFGSVPDFGQTETGVRFSDVRPSSPAAK
                     AGLQAGDVLVHFGDKSINNLYDFTDALRRSKVGDVVEVTVLRNGQPLKVSVTLEQRK"
     gene            2384..3490
                     /locus_tag="VFR42_09690"
     CDS             2384..3490
                     /locus_tag="VFR42_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369471.1"
                     /translation="MRRILSLTLLALSFGSITAVVLRSQPPAPAPVQAPADTSLTTPE
                     ERKHLKNVRQLTFGGQNAEAYFSADDTQLIFQHQGASVPCDQIYSIAVDAADGKAAIP
                     KLLSTGKGRTTCSYFFPSGNRILFSSTHAASAACPPKPDYSRGYVWPIYNTYRIYTAK
                     PDGSGLKLLSDAPGYNAEATITRDGKHIAFTSTRNGDLDIYTMDADGKNVKQLTHELG
                     YDGGPFWSYDGKKIVYRAEHPQTPEQIADYKDLLGKGLIRPGNLEIWVMNADGSNKHQ
                     VTHNGAANFAPYWLPDGKRIIFASNQADPKNGRDFDLYIINEDGSGQQRITFHPDFDA
                     FPMFTSDGKRLVWASNRNGKEPHETNIFLADWVE"
     gene            complement(3559..>4218)
                     /locus_tag="VFR42_09695"
     CDS             complement(3559..>4218)
                     /locus_tag="VFR42_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369472.1"
                     /translation="HSLDYGQNATTFSDTNDLLVPTDIRGEYGNSIFDVRNRFVASAV
                     AESPWHAPGLLGYLVNDWLLAPIYSSQSGLPYSLVTSGTPTFTTPADASGTTITYSGL
                     GASINGSNGRKGIAAIGRNSFRQKRTINMDLRLSKRVRFGERYAVELLGEAFNIFNHQ
                     NVTVVNNTGYIIGSTAVDPVTKAVNATLNFNSSFGSVSNTNSNFAFSPRQIQIGFRFL
                     F"
BASE COUNT          882 a         1360 c         1094 g          882 t
ORIGIN      
        1 gaccgtcgtc ggcagcgctc ttcgcccgac cgcacgggcc gcttccagat cttccgtcgc
       61 ggccagcgcg cgaatccttt gctccgcacg ccgcaactgt tctttcaatt ccgcgaagat
      121 cttcgccgcg tttgcgccgg gttccaactc gcgtcttaat tgctccagtt gcgcctcgct
      181 gagctgcctg ccttccgcat aggtcagcaa tcggtcaacg cttcgcgcaa tatgccggat
      241 ctgaaacgct actggcgcca ccccgtccgg ccgtgcattc agttgcgcgt cgctcagcgc
      301 gccgcagaat ttttccaggt cttcttccgc aagctgcagg gcgtggagca cagcccgggc
      361 cacggcgggg acgtcgccca gcgttccgcg cagccatggt tccgattgtt ttgtctctgt
      421 cattctgtcc gctttctccg cgtgagtttg ctatgatttt ccttcgtcat catcgccaga
      481 ttctattgga atctccctag aatgctgaac ttgattgcca tcaccggcgc gctccgcaga
      541 ccagccatcc gcgcctcgct tcttgcttgg gtggccgctg cttccgcgat ttttctcgcg
      601 ccgcaatggt tgctcgcgca gtccgcacca tctacggctt ccgctgattt gaccaggtat
      661 cttgacgatg tgaaggcact ttcctcgccg gaaatggaag ggcgcggcga tggcacgaag
      721 ggtctaacgc tcgccgaaca cctcattgtc gaacgctaca aatctctcgg cctcgaaccc
      781 gccggcagca aaggctatct gcaatcgttt cgcgtggtca ccggccgcaa aatccagggc
      841 ggaacaaaaa tgtccggcca cgccaacggt ggaaaaattg aaacctacgc cgtccacgaa
      901 gactttatcc cgctcaactt ttcttccact ggcaacgcca gagcctctgt ggtctttgcc
      961 ggatatggca tcactgctcc tgaattcggc tacgacgatt ttcacgatgc cgatgtgcgc
     1021 gacaaaatcg tcctggttct ccgcggtgaa ccggagtcta tctccgagaa agctccgagc
     1081 gccggccaca ccacccacgc atcgctcatc accaaagcca tcaacgccaa aaatcacggc
     1141 gctgccgccc tgattgtcgt caatggcaag ctttcgcacg gcgattccga cgaactgcgt
     1201 ccgtttggcg acgccgaagg ccccgaagaa gcgggcatcc tctgtctgca ggtcaaaacg
     1261 cccgtcgccg atgaatggct gcgccccaca ggcctcacta gcgctgagtt gcaagactcc
     1321 atcgaaaaag aaaaaaactc cggcctctcg atccctctcg attctaccgt tgacgtcacg
     1381 ctgaaaatcg aaaccctgcg cgccaccgtg aacaatgtcc ttgcgtatct gcccggcaaa
     1441 tccgacgaat acgttattgt tggcgcgcac tacgaccatc tcggcaaagg cgattcgcat
     1501 tctctcgcgc cttcctcaat tggccagatt catcctggcg ccgacgacaa cgcttccgga
     1561 actgccggag tgctcgagct cgcgcgcatt gtcgcgccgc tcgccggcaa actcgatcgc
     1621 ggcatcctat tcgccaattt cgcgggagaa gaactcggcc tcctcgggtc cgccgaatgg
     1681 gttaaggaac ccactcgccc gctcgacaaa gccgtggcca tgctcaatat ggacatgatc
     1741 ggccgcatca aggacgacaa agtgtttatc ggcgggatcg gcaccggcac gacgctcaag
     1801 tccgctgtcg aagacgcggc caatggcagc agcttgcatt tcgagtattc cgagggcggc
     1861 tactcctcca gcgatcacac ttccttcgtt accaagaaaa tccccgtgct ctttttcttt
     1921 tccggcctgc acagtgacta ccacaagcct tccgacacct gggacaaaat caacgctgcg
     1981 tcagccacgc aactcctgaa tttggtttca aaagtcactg ttgaactcgc cgaagctgcc
     2041 gaaaagccaa aattcgtcgc ggtcgtcgaa aatcctcatg tgggcggcgt ctccagcggc
     2101 ggcggttacg gaccttactt tggctcggtc cccgattttg gccagacgga aaccggcgtc
     2161 cgcttctccg acgttcgccc cagttcgccc gccgcaaaag ccggcctcca ggccggcgat
     2221 gtgctcgttc acttcggcga caaatccatc aataatcttt acgacttcac cgacgcgctc
     2281 cgccgcagca aagtcggcga tgtcgtcgaa gtgaccgtgc tccgcaatgg ccagccgctc
     2341 aaagtttccg tcactctcga acagcgaaag tagggcttca cgaatgcgca ggattttgtc
     2401 ccttactttg ctcgcattaa gcttcggctc aatcactgct gtcgtgctgc gttcgcagcc
     2461 acccgcgccc gctcccgttc aagccccggc cgacacttcg ctcaccacac cggaagagcg
     2521 aaagcatctc aagaatgtcc gccagcttac cttcggcggt caaaatgccg aagcctactt
     2581 ttcggcagac gacacacagc tcatctttca gcaccaaggt gccagcgtcc cctgcgatca
     2641 gatttactct attgccgtgg acgcggccga cggaaaagct gccatcccga aattgctcag
     2701 caccggcaag ggccgcacca cttgcagcta tttctttccg tccggcaatc gcattctttt
     2761 ttcgtccacg cacgctgcca gcgccgcatg tccgcccaag cccgattatt ctcgcggcta
     2821 cgtctggccc atctacaata cctaccgcat ctacaccgcc aagcccgacg gcagcggctt
     2881 gaagctactc tccgacgcgc ccggctataa cgccgaagcc accataacgc gcgatggcaa
     2941 gcacatcgcc ttcacgtcca cgcgcaatgg cgacctcgac atctacacca tggacgccga
     3001 tggcaagaac gtgaagcagc ttacccacga acttggttac gacggcggcc cgttctggtc
     3061 ctacgacggc aagaaaatcg tctaccgcgc cgagcatccg caaacgccgg aacaaattgc
     3121 cgactacaaa gacttgctcg gcaaaggctt gattcgtccg ggcaatctcg aaatctgggt
     3181 gatgaacgcc gacggcagca acaagcatca ggtcacgcat aatggcgccg cgaacttcgc
     3241 tccctattgg ctgcccgacg gcaagcgcat cattttcgct tccaatcaag ctgatcccaa
     3301 gaacggccgc gacttcgatc tctacatcat taacgaagac ggctcaggcc agcagcgcat
     3361 caccttccat cccgacttcg acgcctttcc gatgttcacc tccgacggca agcgactcgt
     3421 ctgggcttcc aaccgcaacg gcaaagaacc gcacgaaacg aacattttcc ttgccgattg
     3481 ggtggaataa ctagtctgct aaaaaaaaac ggcccgcatg aagcgggccg cttgatgctt
     3541 tttgatttcg acggcggcct agaacaagaa ccggaagccg atctggattt ggcgaggcga
     3601 aaacgcgaag ttgctgttgg tgttactgac actcccaaag gatgagttga agttgagcgt
     3661 ggcatttacg gccttggtca cggggtcgac ggccgtactt ccgatgatgt agcccgtgtt
     3721 gttcacgacc gtcacgttct ggtggttgaa aatgttgaaa gcctcgccga gaagttcgac
     3781 agcgtagcgc tcaccgaaac ggactctctt cgaaaggcgc aagtccatgt taatggttcg
     3841 cttttgacgg aaactgttgc ggccaatcgc agcaattcct tttcggccgt tggagccatt
     3901 gatgctggcg cccaatcccg aataggtgat agtcgtgccg ctggcatcgg ccggcgtggt
     3961 aaaggttggc gtcccggagg ttactagcga gtacgggagc ccgctttgcg acgagtagat
     4021 gggcgcgagc agccagtcat tcaccagata acccaggagg ccgggcgcgt gccacggact
     4081 ctcagcgacc gcgctggcca cgaagcggtt acggacatcg aagatggagt ttccgtattc
     4141 gccgcggata tccgtcggca cgagcaggtc attggtgtcc gagaaagtgg tcgcgttctg
     4201 cccgtagtcc agggaatg
//
LOCUS       DASTPM010000548         5983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_538340, whole genome shotgun sequence.
ACCESSION   DASTPM010000548 DASTPM010000000
VERSION     DASTPM010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538340"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VFR42_09700"
     CDS             complement(<1..438)
                     /locus_tag="VFR42_09700"
                     /inference="COORDINATES: protein motif:HMM:NF002696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HEU0369473.1"
                     /translation="MIEQDLQKIALQEKRLQFTEFNAAVAWELGARLRSAALQRGAAI
                     AIDIQLHGHLLFACSMPGTTPDNWEWIRRKRNVVMRYHRSSYAIGLKHALAGSTLEGT
                     TGLDLRDYSTHGGCFPILLAGTGCVGTIAVSGLPQREDHSLVVS"
     gene            566..2023
                     /locus_tag="VFR42_09705"
     CDS             566..2023
                     /locus_tag="VFR42_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0369474.1"
                     /translation="MQPGRENNRAEVTKEESLAGTPAEAQLETRESGLKSKLPPRQVT
                     MIGLGSTIGTGLFLGSAISVKQAGPAVIVSFAAGALIALTVMWALAEMAVEHPATGSF
                     GLYAEMYLSAWAGFAVRYTYWLCLVMVVGSEVVAAAIYCAFWFPQVPAPVWIAIFSVA
                     LVVINTLSIEEFGSIEYWFAMIKVATILAFLVLGAMLLFGIGFPRIGTANFTAHGGFM
                     PNGWRGVGLGVIMAIFSYLGLEIVGTTAGEAADPKKAVPRALRRTLGWLAVFYLGGLA
                     LVVGIVPWTEIGLGQSPFVRVFERVGIPAASHIMNFVVLTAALSSALANLYFCARLLF
                     SLARGGFAPTGLGKLSKSGMPVAAVLASSVGLIAALVLSGIFQRTAFVFLIGVAFFGG
                     PFIWLMTLATHIAFRVKRAKENRAIVKIAPLGLWSSALGIVALVAVLASTWWIPDFHV
                     ALLAGPPWLAFLTVCYFIWRKFQSSKTAAGAGPVG"
     gene            2016..3227
                     /locus_tag="VFR42_09710"
     CDS             2016..3227
                     /locus_tag="VFR42_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0369475.1"
                     /translation="MDKPMDELLKWRQEFPILERATYLISNSLGAMPRGVRDGLNAYA
                     DAWAGRGVRAWEEGWWDLAGNVADQMAPLIGAGPGEISLHQNVTTTQAVIASCFDWRG
                     PRNKVVMTELEFPSVQYFYHGQRRHGARVELVPSGNAVRLDLDAFLAAIDETTLLVPI
                     SLVLFRSSFIVNARAIIEKAHRVGAHVVLDVFQATGTIPVDVRGLGADFAVGGVLKWL
                     CGGPGVAYLYVREDLRPKLRPALTGWLAHRRPFEFEAGAIDPRDDSYRFLNGTPHIPA
                     LYACRPALEILNEVEAKAIREKSVRLTTRVIEGAKARGWRVNTAEDPAERAGTVSVEC
                     PHAIEVSRELLARDILIDYRPQAGIRISPHFYNREEECDFAVSQIEEILRTRAWEKHA
                     VGTAMAEQRKR"
     gene            3224..3985
                     /locus_tag="VFR42_09715"
     CDS             3224..3985
                     /locus_tag="VFR42_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HEU0369476.1"
                     /translation="MSTAAAGSDGRPIELSIIVPSYNEELRLPASLERIAAYVAESGR
                     CTEVLVVDDGSKDKTAAVAAAYADRIANLRVLKNGENRGKGYSVRHGMLEAKGEVVLF
                     TDADLSAPIEEADKLLAAMGEYDVSIGSRAMDRSLIKVHESAFREFAGIVFNRIVRIV
                     LRLPFVDTQCGFKAFRRERCWIIFEQQRIRRFGFDPELLYLARHHGLKSVEIPVRWSH
                     SPATKINMMRDSIQMFVDVFTIRWNALRGCYPREK"
     gene            4056..4670
                     /locus_tag="VFR42_09720"
     CDS             4056..4670
                     /locus_tag="VFR42_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369477.1"
                     /translation="MPLPEPIRAASRAPIPIDARAADHLRYIRETMQSAGEFTAVPGW
                     GGIAMGVTALAAAVFASRQHTPERWLSIWLMEAFVAVAIAAPAAATKAHRANAKLLSG
                     PGRKFLLSFAPPILVGGLLTFALSRAGLHALLPGVWLLLYGTAIVTGGAFSVRAVPIM
                     GLCLILLGSAALFVPLAIGDLFMAMGFGFVQIGFGIWIVQRHGG"
     gene            4733..5071
                     /locus_tag="VFR42_09725"
     CDS             4733..5071
                     /locus_tag="VFR42_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU0369478.1"
                     /translation="MGPLKEHRDHKREAVSADLDRLIHERIRLGIVSALAVNRSLTFN
                     ELKALLKTTDGNLSVHARKLEEADYILCEKSFDGRLPKTEYRLTAAGRKALERYLNHM
                     EALIRATREG"
     gene            5147..5911
                     /gene="uppS"
                     /locus_tag="VFR42_09730"
     CDS             5147..5911
                     /gene="uppS"
                     /locus_tag="VFR42_09730"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU0369479.1"
                     /translation="MTVQQVILQHILNAPPVTPAPSLHVAIITDGNGRWATSRGLPRS
                     AGHRAGAEASRAIIEAAPKLGIHTLTLFALSAANWKRPAAEVNSILRLLHEYLMAETS
                     RCMENGVRISVPGRRDRIPMTLRQAIMDAEAATAGGTRLHLRLAIDYSAREAIYLAAC
                     RYYKATELSPESFGGVLSEVTRGGATDVDLLIRTGGEQRLSDFLLWECAFAEFVFLEK
                     RWPDFTVADLRAAVEEFSRRERTRGSLPQDVVAQKI"
BASE COUNT         1101 a         1478 c         2131 g         1273 t
ORIGIN      
        1 cgacaccacg agcgagtggt cttcgcgttg cgggaggcct gaaacggcga tggtcccgac
       61 gcatccggtg ccggcaagca ggatgggaaa acagccgccg tgagttgaat agtcgcggag
      121 atcaaggccg gtagttcctt ccagcgtgct gccggccagc gcgtgcttca gcccgatggc
      181 gtaggagctg cggtgatagc gcatcacgac attgcgcttg cggcggatcc attcccagtt
      241 gtcgggcgtg gttcccggca tgctgcaggc aaagagaaga tggccatgga gttggatatc
      301 gatggcgatt gcggcaccgc gctgtagggc ggccgaccgc agcctggcgc caagctccca
      361 ggccacggcg gcgttgaact cggtgaattg caggcgcttt tcctgtagcg cgattttctg
      421 aaggtcctgc tcgatcatac gtctcctgaa gcgggacgct atcttaacgc gaaacctcgc
      481 tcgggcggac ccgatggctt gtgagacggc ccggcggaaa aggcgttagg ccgcgctggc
      541 gctggtttgc tacgatgcgg ttcccatgca acccggccga gagaacaacc gagcggaagt
      601 caccaaagaa gagagtctgg ccgggacgcc ggccgaagcg cagctggaaa cgcgcgaaag
      661 cgggctcaag agcaagctgc cgccgcgcca ggtgaccatg atcgggttgg gctcgacgat
      721 cggcaccggg ttgtttttgg gaagtgctat cagcgtgaag caggcggggc cggcggtgat
      781 tgtgagtttt gcggcaggcg cgctgattgc cctgacggta atgtgggcac tggcagagat
      841 ggcagtggaa catccggcga cgggatcgtt cgggttatac gcggagatgt acctgagtgc
      901 gtgggcggga tttgcggtgc gctacaccta ttggctgtgc ctggtgatgg tggtgggaag
      961 tgaagtggtg gcagccgcga tttattgcgc gttctggttt ccgcaggtgc cggcgccggt
     1021 gtggatcgca attttttccg tcgcactggt ggtcatcaat acattgagca tcgaggaatt
     1081 tgggtcgatt gagtattggt tcgcgatgat caaggtggcg acgattctgg cgtttttggt
     1141 attgggagcg atgctgctgt ttggcattgg atttccgcga atcggcacgg cgaacttcac
     1201 ggcacatggt ggattcatgc cgaatggatg gcgcggcgtc gggctgggcg tgataatggc
     1261 gattttcagc tatttaggat tggagattgt gggcacgaca gcgggcgaag cggctgatcc
     1321 taaaaaggcc gtgccacggg cgttgcggcg gacgctgggc tggctggcag tgttctattt
     1381 gggcggattg gcgctggtag tgggaattgt gccgtggacg gagatcgggc tggggcagag
     1441 tccttttgtg cgcgtgtttg aacgggtggg aattccggcg gcaagccaca tcatgaattt
     1501 cgtggtgctg acggcggcgc tttcgagcgc actggccaac ctgtattttt gcgcgcggtt
     1561 gttgttctcg ctggcgcgcg gaggatttgc tcccacgggg cttggaaaac tgagcaagag
     1621 cggaatgccg gtggcggcgg tgctggcctc cagcgttgga ttgatcgcgg cgctggtgct
     1681 ttcgggaata ttccagcgga ccgcgttcgt atttttgata ggcgtggcat tttttggcgg
     1741 accgtttatc tggctgatga ctctggccac gcacatcgcg ttccgtgtaa agagggccaa
     1801 ggaaaatcgg gcgattgtaa agattgctcc tcttggattg tggagcagcg cgctgggaat
     1861 cgtggcgctg gtcgcggtgc tggcgtccac gtggtggatt cccgattttc acgtggcgct
     1921 gctggcggga ccgccgtggc tggcgttcct gacggtgtgc tactttatct ggagaaaatt
     1981 tcagtccagc aaaaccgctg caggagcagg cccggttgga taaaccgatg gatgaattgc
     2041 tcaaatggcg ccaggaattt cccatcctgg agcgggcgac atatctgatc agcaattcgc
     2101 tgggagcgat gccgcgcggt gtgcgcgatg gactgaatgc gtatgcggat gcgtgggccg
     2161 ggcgcggtgt gcgcgcctgg gaagaaggtt ggtgggatct ggcaggcaat gtggcggacc
     2221 agatggctcc gctgattggc gccgggccgg gcgaaatttc tctgcaccag aacgtgacga
     2281 ccacgcaggc ggtgattgcg tcgtgttttg attggcgcgg accgcgcaac aaagtggtga
     2341 tgacggagct ggaatttccg tcggtgcaat atttttatca tgggcagcgg cggcacgggg
     2401 ctcgggtgga actggtgccg agcgggaatg ctgtgcggct tgatctggac gcgtttctgg
     2461 cggcgattga tgagacgacg ttactggtgc cgatttcgct ggtgctgttc cgcagctcgt
     2521 tcatcgtgaa cgcgcgggcg attatcgaga aggcgcatcg cgtgggcgcg cacgtggtgc
     2581 tggacgtgtt ccaggcgacg ggaacgattc cggtcgacgt gcgaggactg ggcgcggatt
     2641 ttgcggtggg cggtgtgctg aaatggctgt gcggcgggcc gggcgtggcg tatttgtatg
     2701 tgcgcgagga tttgcggccc aagctgcggc cagcgctgac gggatggctg gcgcaccggc
     2761 ggccgttcga gtttgaagcg ggagcgattg atccgcgcga cgattcgtat cgctttttga
     2821 atggaacgcc ccatattccc gcgctatatg cctgccggcc ggcgctggag attttgaatg
     2881 aggtggaagc gaaggcgatt agggagaagt cggttcgctt gaccacgcgg gtgatcgaag
     2941 gcgcaaaagc gcgcggctgg cgtgtgaata cggcagaaga tccggcggaa cgagcaggaa
     3001 cggtttcggt ggaatgtccg catgcgatcg aggtgagccg cgagctgctg gcgcgcgaca
     3061 tcttgatcga ttaccggccg caggcgggca tccggatttc gccgcacttt tataaccgcg
     3121 aagaagagtg cgatttcgcc gtttcgcaaa tcgaggaaat tttgcggacg cgcgcctggg
     3181 aaaagcatgc agtcgggacc gcgatggcgg agcaacgtaa acgatgagca cagcagccgc
     3241 cggttcggat ggcagaccca tcgaactgtc catcattgtt ccttcgtaca acgaggaact
     3301 gcggctacca gcttcgctag agcggattgc ggcatacgtg gcggagtcgg gccggtgcac
     3361 agaagtgctg gtggtagatg atggatcgaa ggacaagacg gcagctgtgg cggcggcgta
     3421 tgcggatcgc atagcgaatt tgcgggtgct gaagaacggc gaaaatcgcg gcaagggtta
     3481 cagcgtgcgg cacggcatgc tggaagcgaa gggggaagtg gtgctgttca ccgacgcgga
     3541 tctgtctgcg ccgattgaag aagcggacaa gttgctggcg gcgatgggcg agtacgacgt
     3601 gtcgatcggg tcgcgcgcga tggatcgcag cttgatcaaa gtacacgagt cggctttccg
     3661 cgagtttgcg ggcattgtgt tcaacaggat tgtacggatt gtgctgcggc tgccgtttgt
     3721 agatacgcaa tgcgggttca aggcgttccg gagggaacgc tgctggatta tttttgagca
     3781 gcagcggatt aggcggttcg gattcgatcc agagctgctg tatctggcgc ggcaccacgg
     3841 gctgaagtcg gtggaaattc cggtgcgctg gtcgcattcg ccggcgacga agatcaacat
     3901 gatgcgggac agcatccaga tgtttgtgga cgtgtttacg atccggtgga atgccctgcg
     3961 gggctgctat ccgagggaga agtgaggcgg gaaggggtca ctttataata caaagtactt
     4021 gacaaaggta cgccgacaga cgtatactct ccttcgtgcc gcttcctgag ccaattcgcg
     4081 ctgcttcccg cgcaccgatc ccgattgatg cgcgcgcggc agaccacctg cgctacatcc
     4141 gcgaaaccat gcagagtgcc ggggagttta cggcggtgcc cggctggggc gggattgcca
     4201 tgggagtgac ggcgctggcg gcggcggtgt ttgcttcgcg gcagcatacc ccggagcggt
     4261 ggctctccat ctggctgatg gaagcgtttg tagcggtagc gatagcggcg ccggctgcag
     4321 ctaccaaggc acatcgggcc aatgcaaagt tgttgtcggg accggggcgg aaatttttgt
     4381 tgagttttgc tccgccaatt ttggtgggtg gattgttgac gttcgcgtta tcgcgggcgg
     4441 ggttacacgc tctgctgccg ggggtatggc tgctgcttta tggcacagcg attgtgaccg
     4501 gtggggcgtt ttccgtgcga gccgtgccga tcatggggct ttgcctgatc ctgctaggct
     4561 cggcggcgct ttttgtgccg ttggcgatcg gcgacctttt tatggcgatg ggctttggat
     4621 tcgtgcagat tggattcggg atctggattg tgcagcgaca tggaggctga acggattttg
     4681 catgtctaag tcatcgacaa ttcgaaatga acacgcgggg cggccggcct cggtgggacc
     4741 tctcaaggaa catagggacc acaagcgcga ggcggtgtcc gccgatctgg accggctgat
     4801 tcacgagcgg atacggctgg gcatcgtgag cgcgctggcg gtgaaccgct cgctgacgtt
     4861 taatgaactg aaggcactgc tgaagactac cgacggcaat ttaagcgtgc acgcgcgcaa
     4921 gctggaagag gcggattaca tcctttgcga aaagtctttc gacgggcgct tgcctaagac
     4981 ggaataccgg ctgacggcgg cggggcggaa ggcgctggag cgctatttaa atcatatgga
     5041 ggcgctgatc cgggctacgc gcgaaggata aaaaaatttg cctagttgct ttgcggtata
     5101 aagttgtttg caccaagctt ccccgcggcg gagaaggaac atttccatga cggttcagca
     5161 ggtcatcttg cagcacattc tcaacgcacc gccggtcacg cccgcgcctt cgctccacgt
     5221 ggccatcatc accgacggca acgggcgatg ggcaacttcg cgggggttgc cgcgttcggc
     5281 ggggcaccga gcgggcgcgg aagccagccg ggcgatcatt gaagctgcgc cgaagctggg
     5341 cattcacacg ctcacactgt ttgcgttgtc tgcggcgaac tggaagcggc ctgccgcaga
     5401 ggtcaattcg attctgcgtc tgctgcacga atacctgatg gcagagacat cgcgctgcat
     5461 ggaaaacggc gttcggatca gcgtgccagg aaggcgcgac cgaattccca tgacgctgcg
     5521 gcaagcgatt atggatgcgg aagcggcaac cgcgggcggc acgaggttgc acctgcggct
     5581 ggcgattgac tattcggctc gcgaagcgat ttacctggca gcgtgccggt attacaaggc
     5641 gaccgaactt tcaccggagt cttttggcgg tgtgctgtcc gaggtcacgc gtggcggggc
     5701 gaccgacgta gacttgttga tccgcacagg cggcgagcag cggctctccg actttctctt
     5761 gtgggaatgc gcgtttgcgg agtttgtatt cctggaaaaa cgctggccgg attttacggt
     5821 ggccgatttg cgggcggcgg tggaagagtt ttcccggcgt gagcggacca gaggttcgtt
     5881 gccgcaggac gtagtagcgc aaaagatata agccaggcac cttccggcgc gtcccgacaa
     5941 cagcttcgcc tggcgagatc tgatgagcgc tgccggttgg tac
//
LOCUS       DASTPM010000549         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_970694, whole genome shotgun sequence.
ACCESSION   DASTPM010000549 DASTPM010000000
VERSION     DASTPM010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970694"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFR42_09735"
     CDS             complement(<1..802)
                     /locus_tag="VFR42_09735"
                     /inference="COORDINATES: protein motif:HMM:NF023015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU0369480.1"
                     /translation="MENFFSSQTTGREALSWQIHVDAPVAQHYVACDTQLDGIYAKAK
                     AQQWDIEDYDWSFELAPDNPLQLPDATLLVYGTPVWEDLDSTGRSELRRHFQAWTLSQ
                     VLHGEQGALLCAAKLAQGEPSLSGRLCMVAQCYDEARHVEVYARLVNDKIGIRYGMSS
                     SLENLLRQTVSASELDMTNLGMQVLVEGIALSIFQNIVAYSADPTIKQMISQVQRDEA
                     RHFAAGRITLARIYRGELTSAEMAIREEFVCESMTTMYEHLCADDIWAP"
     gene            complement(810..1196)
                     /locus_tag="VFR42_09740"
     CDS             complement(810..1196)
                     /locus_tag="VFR42_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0369481.1"
                     /translation="MPAIPLRNNTYVLEVFDSNLSGSTFEDCLLANANLKYTSFEKAR
                     IQMVRFDSVQFTSVSFEKASIERGNFVNCSVKNGRYTGMTIEGIQVTELLEMYFATHP
                     EKREAMREEARISGALEDARAAGPGG"
     gene            complement(1249..2484)
                     /locus_tag="VFR42_09745"
     CDS             complement(1249..2484)
                     /locus_tag="VFR42_09745"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU0369482.1"
                     /translation="MSYSLILADRATGLNYTNDMNGESVLSRHPGRPLVFLDERFKGR
                     LQELPNCVYEYMPYMTSEAVIDRARHYQQTAGLSSVCTFDEYLVYLAAEVRTALGLPG
                     MKVEQARRFRDKVLMKTLLRAAGVRVPEFVASCADCTAVNALLARHGPIVVKPHDGAG
                     SKGVTFFTEAAALDAFLERLSEPGQYEAEEFIAGTLYHVNSVVGGGKVLYSGVGIYLP
                     GKANIDFSDGQSFSTVTVTDGALHDALKRFAETVIAVLELTSGVTHMEAFVTQNSEIV
                     FCEIAARPPGGGIVTMLNMQHGIDLWTACMLCEEGMAHRALAARSERSDRVGVCGLRN
                     AAMGIVELDPEAATAHADWVRYVKLSVSNGQFRAPSAHCTDFTAMVVLTASSTGQFRE
                     RVDEIERRYATHLRLVAMD"
     gene            complement(2495..>3100)
                     /locus_tag="VFR42_09750"
     CDS             complement(2495..>3100)
                     /locus_tag="VFR42_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369483.1"
                     /translation="SLRGDEEQLLVVSAADKWLPPFDVGFAPLVQYGDAAAAACIGYA
                     QSGRPALAVIEGIVTGWQPEPAVVWERSLDATCEAMVAMVAGLIATLMLRHRIDAGKL
                     SGVIGEAYGCALPARVLAAAGLPPPLAEPEPPENHLSSADFVHCLLRGVRAASAADRP
                     GHYLIWTASPFGSAGVALVRCHPHAVTARSGGTFDLSASIF"
BASE COUNT          650 a          968 c          883 g          599 t
ORIGIN      
        1 gcggcgccca gatgtcatcg gcgcacaagt gttcatacat agtggtcatg ctctcgcaaa
       61 cgaactcttc ccggatggcc atctcggcgc tggtcagctc gccacgatag atacgcgcca
      121 gggtgatgcg gccggccgca aaatggcgcg cctcgtcacg ctggacctgc gaaatcatct
      181 gcttaattgt cgggtctgcg ctgtaggcga cgatgttctg gaaaatggac agcgcaatcc
      241 cctcgaccaa cacctgcatg ccgagattcg tcatgtccaa ttcgctggca ctgacggtct
      301 gacgcagcag attttcgagc gagctgctca tgccgtaacg aattccaatc ttgtcgttca
      361 cgaggcgtgc atacacctca acgtgacggg cctcgtcata gcactgtgcc accatgcaca
      421 accggccgct cagtgagggc tcgccctgcg caagtttggc ggcgcacaac aaggccccct
      481 gctcgccatg cagcacctgg gatagggtcc aggcctggaa atggcgccga agctcgctgc
      541 gaccggtaga gtcgaggtct tcccatacag gcgtgccata cactagcaag gtcgcatccg
      601 gcagttgcaa gggattgtcg ggggcgagtt cgaaactcca gtcgtagtcc tcgatatccc
      661 attgctgggc tttagccttg gcataaatcc catccaactg ggtatcgcag gcgacatagt
      721 gctgtgcgac cggggcatcg acatgaatct gccaggagag cgcctcccta ccggtcgtct
      781 gcgaagaaaa aaaattctcc atcacagtcc tagccgcccg ggccggcggc gcgcgcgtcc
      841 tcgagcgcgc cgctaatgcg agcctcttcc cgcatcgctt cacgcttttc tggatgggta
      901 gcgaaataca tctccaggag ctcggtgacc tggatgcctt cgatggtcat gcctgtatag
      961 cgcccgttct tgactgagca gttgacaaaa ttgccgcgtt caatgctggc cttttcaaag
     1021 ctgacgctgg tgaactggac actgtcgaac cggaccattt gaatccgggc cttttcgaac
     1081 gatgtgtact tcagattagc gttggccagg aggcagtctt cgaacgtgga cccgctcaga
     1141 ttggaatcga atacttcaag gacgtaagta ttgttgcgta acggaatggc aggcatcgga
     1201 actcctcatt gagtgttaaa aaacatgcaa ctggatgaga cggacgggtc aatccattgc
     1261 taccaaccgc aaatgtgtgg catagcggcg ctcgatttcg tcgacgcgtt cgcgaaactg
     1321 tccagtgctc gaggcggtca ggacaaccat tgcggtaaag tcggtgcaat gcgcactggg
     1381 cgcccggaac tggccattgc tgacggacag cttcacgtag cgtacccagt ccgcgtgtgc
     1441 cgtggccgct tccggatcaa gttcgacgat ccccatggcc gcgttgcgca gtccacaaac
     1501 gccgacccgg tcgctgcgtt cgctgcgcgc ggccagcgcc cggtgcgcca tgccttcttc
     1561 gcataacatg caggcagtcc acaaatcgat accatgttgc atattgagca tcgtaacgat
     1621 acctccgccc ggtggtcgcg ctgcgatctc gcaaaacacg atctcgctgt tctgcgtgac
     1681 gaatgcttcc atgtgagtga cgcccgaggt cagttccagc accgcgatca cggtttcggc
     1741 aaagcgcttc agtgcgtcat gtagtgcgcc gtccgtgacc gtaacggtgg agaacgactg
     1801 cccgtcggag aaatcgatgt tagccttgcc cggcaaatag atgcccaccc cgctatagag
     1861 caccttgccg ccgcccacca ccgaattgac gtgataaagg gtgccggcaa tgaattcttc
     1921 ggcttcgtac tgccctggct cactcaaacg ctccagaaac gcgtcgagtg ccgccgcctc
     1981 cgtaaagaac gtcacgcctt tagatcctgc cccatcgtga ggcttcacca cgatggggcc
     2041 atggcgtgca agcaaggcat tcacggcggt acagtccgcg caggaggcga cgaactccgg
     2101 cacccttaca ccggccgcgc gcaacagggt cttcatcaac accttgtcgc ggaaacgtcg
     2161 cgcctgctcc accttcatac ccggcaggcc gagcgcggtc cgcacctccg cggccaggta
     2221 gactaggtat tcatcgaacg tacacaccga gctcaggccc gcagtttgtt gataatggcg
     2281 ggcacggtcg atgaccgcct cgctggtcat gtacggcatg tattcgtaaa cacagtttgg
     2341 cagctcctgt agacgtccct tgaatcgttc atcgagaaag acgagtggcc gtcctggatg
     2401 acgcgacagc acgctctcgc cgttcatgtc attggtatag ttcagtccgg tagcacgatc
     2461 ggccagaatt aaggaatagc tcaacgtaga ctcctcagaa aatagaagct gacaaatcga
     2521 aggtgccgcc gctacgggcg gtgacagcgt gaggatggca acgtaccagc gccaccccgg
     2581 cgctgccgaa cggcgaggca gtccaaatca gatagtggcc cggacggtcg gccgctgaag
     2641 ccgcgcgaac cccacgcaac aggcaatgga cgaaatcggc cgagctcaag tgattttccg
     2701 gcggctccgg ttcagccagg ggaggaggca aaccggccgc cgccagtacg cgcgccggca
     2761 gcgcgcatcc ataggcttcg ccgatcactc cggacagctt gcccgcgtcg atccggtgcc
     2821 gcagcataag cgtggcgatc agcccggcca ccatcgccac catcgcttcg caggttgcat
     2881 ccagagatcg ttcccagacc accgccggct ccggttgcca tccggttaca ataccctcga
     2941 tcaccgccag cgcgggccgg cctgattgcg cgtagccgat acaggcggcc gcggctgcgt
     3001 cgccgtactg caccagcggg gcgaatccga catcgaacgg aggaagccat ttatcggccg
     3061 cgctgacgac caacaactgc tcttcatctc ccctgagcga
//
LOCUS       DASTPM010000550         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_365430, whole genome shotgun sequence.
ACCESSION   DASTPM010000550 DASTPM010000000
VERSION     DASTPM010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365430"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VFR42_09755"
     CDS             <1..1014
                     /locus_tag="VFR42_09755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0369484.1"
                     /translation="WADLFNEYMRPPGIIARALKEGAAAILWTGARERLLLYRHTNSL
                     NGEVDKISQAVVAREDALRLARLVAANQGSSQGKVRVKLTMPNEISGPVEQENVVGEI
                     RGYEKPDEVVILGAHLDSWELGTGALDNGCNAALVIEAARAIKAMGVLPKRTIRFILF
                     SGEEQGAIGSHEYVKAHAAELDKIRAVIVFDDGSGRTSGFSLGGRTDTDAPLRQILKP
                     LETWNVGEHTPDASFGTDNFDFLLEGVPTFVANQEVSNYLANYHAASDTFDKVDQREL
                     KMNTVIAALTAWGIADRSEPIGKRQTRSELDAMVKQTGLDQQMQLLGYWEQWQSGARG
                     RKP"
     gene            1040..1552
                     /gene="ruvX"
                     /locus_tag="VFR42_09760"
     CDS             1040..1552
                     /gene="ruvX"
                     /locus_tag="VFR42_09760"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU0369485.1"
                     /translation="MSPSTTQPAKTAKHSGQILAIDYGRVRIGLAIADAQQGLAQPLA
                     TLERINRNEDMRRLRELARDYSVKLVVVGLPLRLDGTRGEMAEEAERFAKRVRKQIGI
                     PVEMADERLTSWEAERQLEEQFGRKSKLSDSASKKKPVEKFSADSMAAAIILRDYLQH
                     RTATSEGAAE"
     gene            1549..>2156
                     /locus_tag="VFR42_09765"
     CDS             1549..>2156
                     /locus_tag="VFR42_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU0369486.1"
                     /translation="MKFLARVFLLLLLMGAAAAGYLWYCVEEPFGTYPAEGIFVDIPH
                     GASRRTVARQLEKEGVVRSAIAFEIYARRHPKRTLQAGEYFFDHAISGKDVFSTIASG
                     NVYQRPFTVREGETMFDIARELEAGKFMTADDFLVAAKNPELIHDLVPQAETLEGFLF
                     PATYNLPRQPVAKELVAEMTQKFREQWSQIDPAKSDPTGVGHG"
BASE COUNT          498 a          607 c          656 g          395 t
ORIGIN      
        1 tgggcggatc ttttcaacga gtacatgcgg ccgccgggca tcatcgcgcg cgcgttgaaa
       61 gaaggagccg ccgccatctt atggacggga gcacgcgaac ggctgttgct ctatcgccac
      121 accaattcgc tgaacggcga ggtcgacaaa atttcgcaag ccgtggttgc gcgggaggac
      181 gcactgcgcc tagcgcgcct ggttgcggca aatcaaggtt ccagccaggg aaaagttcgc
      241 gtgaagctga ccatgcccaa cgaaatcagc ggtcctgtcg agcaggaaaa tgtggtgggg
      301 gaaattcgcg gatatgaaaa gcccgacgag gtggtcatcc tcggagcgca tctcgattcc
      361 tgggaacttg gcaccggtgc actggacaac ggctgcaatg cggcgctagt gatcgaagcg
      421 gcgcgcgcaa tcaaggccat gggcgtgctg cccaagcgaa ccatccgttt tattttattc
      481 agcggcgagg agcaaggcgc catcggctct cacgaatacg tgaaagcaca tgccgccgaa
      541 ctggacaaaa tccgagccgt gattgttttt gacgacggca gcggccgcac tagtgggttt
      601 tcgctgggcg gccgcaccga caccgatgca ccgctgcggc aaatcctcaa gccgctggaa
      661 acctggaacg tcggcgaaca cactcccgat gcttcttttg gcaccgacaa ctttgacttt
      721 ctgctggagg gcgtgccgac attcgtggcc aatcaggagg tttccaatta tctggctaac
      781 taccatgcgg cgtccgacac attcgacaag gtggaccagc gcgaactgaa gatgaacacg
      841 gtgatcgccg cgctaacggc gtgggggatc gccgaccgca gcgagccgat cggcaagcgc
      901 cagacgcgca gcgaactcga tgcgatggtc aaacagactg gcctggacca gcagatgcag
      961 ctcttgggtt attgggagca gtggcaaagc ggagcacgag gccgcaaacc gtaggcgata
     1021 tactcatgta gtgagccgaa tgtccccatc cacaacgcag ccggcaaaaa ctgcgaagca
     1081 cagcggtcag atacttgcaa tcgactacgg ccgcgttcgc atcggcttgg ctattgcgga
     1141 cgcacagcaa ggcttggcgc agccgctagc cactctggaa cgcatcaacc gcaatgaaga
     1201 catgcgccgg ctgcgcgaac tggcgcgcga ctattccgta aaactcgtcg tggtgggatt
     1261 accgcttcga ctcgacggta cgcgcggcga aatggccgaa gaggcagagc gattcgcgaa
     1321 gcgtgtgcgc aaacaaatcg gcattccggt agagatggcc gacgagcgat tgaccagttg
     1381 ggaagccgaa cggcaattgg aagaacaatt tgggcgcaag tcgaaattga gcgattcggc
     1441 atccaagaag aagcccgttg aaaaattttc cgcggactcc atggcagcgg ctattatctt
     1501 gcgcgactat ctgcaacacc gaaccgccac ttccgagggc gctgccgaat gaaatttctg
     1561 gccagagttt tcttactgct tttgctgatg ggagccgccg ccgcgggcta cttgtggtac
     1621 tgcgtggaag aaccgtttgg cacttatccg gcggagggca tctttgtaga tattccgcac
     1681 ggcgcctcgc ggcgcaccgt ggcgcgccag ctggaaaaag aaggcgttgt ccgcagcgcc
     1741 atcgcttttg agatttacgc gcggcgccat cccaagcgca cactgcaagc cggcgagtat
     1801 tttttcgatc acgccatcag cggcaaagac gttttctcaa ccattgccag cggcaacgtg
     1861 taccagcggc cgttcacggt gcgcgaaggc gaaacgatgt ttgacattgc gcgagagctg
     1921 gaagcgggca agttcatgac cgcggacgat tttttggttg cggcgaaaaa tccggagctg
     1981 atccacgacc tggtgccgca agccgaaacg ctagaaggct tcctcttccc ggcaacctac
     2041 aatctgccgc gccaaccagt ggccaaggaa ctggtggcgg aaatgacgca aaagtttcgc
     2101 gaacaatggt cgcaaatcga tcccgcgaag tccgatccga ctggagtcgg ccatgg
//
LOCUS       DASTPM010000551         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_509432, whole genome shotgun sequence.
ACCESSION   DASTPM010000551 DASTPM010000000
VERSION     DASTPM010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509432"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFR42_09770"
     CDS             complement(<1..706)
                     /locus_tag="VFR42_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0369487.1"
                     /translation="MNPTPAPAPNPNKALWEKGDFTEIAAFMRDSGDAFAKSLGITPG
                     MRVLDLGCGDGTTALPIARLGADVTGIDIARNLVEAGNRRAAAAGLSNLKFQEGDASN
                     LQGVADHGFDLTVSMFGAMFAPRPFDVAKEMVRVTKPGGRIVMGNWIAGDPTFVSQLL
                     KISASFLPPPPEGFVSPVTWGVDAHVIERFGQAGVAREKISMVKDTYYFTSADRSAAT
                     FMDLFGKFYGPTMNAVD"
     gene            complement(881..1186)
                     /locus_tag="VFR42_09775"
     CDS             complement(881..1186)
                     /locus_tag="VFR42_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369488.1"
                     /translation="MSAKGHRPMNFIRRWRHGAERHQEQLLVKLWTINTLDADDHTQS
                     AQLTPKLALAAKHELKSSLKYLSSAEVESTIQQTVSEKAYQHDVVSNLRRQLNHNRK"
     gene            1599..>2162
                     /locus_tag="VFR42_09780"
     CDS             1599..>2162
                     /locus_tag="VFR42_09780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0369489.1"
                     /translation="MHLWQTLAELRASGAWWLIALRYSIAVVVGFYVLNQVRKPSRWV
                     GRPFLWMMNLSHSPLTDWGLQHIRVVEDFQILDVGCGGGRTIQKLAALAPQGQVSGVD
                     YAQGSVAASRARNRQLIAAGRVSIQEASVSRLPFAENTFDLVTAVETQYYWPDLPNDL
                     QEILRVLKPGGALLVIAESYKKGSANLL"
BASE COUNT          365 a          713 c          595 g          489 t
ORIGIN      
        1 cgtccaccgc gttcatcgtc ggcccataaa acttcccgaa caggtccatg aacgtcgccg
       61 cgctccggtc cgctgatgtg aagtagtagg tatccttcac catcgaaatt ttctcgcgtg
      121 ccacgccggc ttgcccgaat ctttcaatca cgtgagcgtc cacgccccac gtgaccggac
      181 tcacaaatcc ttccggtggc ggcggcaaaa acgacgcgct gatcttcagc agctgcgata
      241 cgaacgtcgg atcgcccgcg atccagttgc ccatcacgat gcgcccgccc ggtttggtca
      301 cccgcaccat ttctttcgcc acatcgaacg gccgcggcgc aaacatcgcg ccaaacatcg
      361 acaccgtcag gtcaaaccca tggtccgcca cgccttgcaa attcgacgcg tcgccttcct
      421 gaaatttcag gttgctcaag ccggcggcag cggcgcgccg gttgcccgct tccaccaggt
      481 tccgcgcgat gtcaatgccg gtcacgtcgg ctcccagccg cgcgattggc aacgccgtcg
      541 ttccgtcccc gcagccaaga tcgagcacgc gcatcccggg cgtgatgccc agcgatttgg
      601 cgaaggcatc gcccgagtcc cgcataaacg cggcaatttc cgtgaagtcg cccttttccc
      661 acagggcctt attggggttc ggtgctggtg ctggagttgg gttcataatg cccttcttat
      721 accacgccgg gcggcgcagt tgtctcgtac gctgtgataa agcgtcacgg ttttgccaca
      781 tcgtcggcag gttttagcgt agcgccgggc tccagcccct gaggaatttt ctttagattt
      841 aacttttaac tttcaactgt cgacggtcta cgtccgcacc ttacttgcgg ttgtggttca
      901 gttgccgccg caggttgctg accacatcgt gctggtaggc cttttcggag acggtttgct
      961 ggatggtgga ctcgacttcg gcggaggaaa ggtatttcag gctggacttg agttcgtgtt
     1021 tggcggcgag tgcgagtttg ggcgtcagct gcgcggactg cgtgtgatcg tccgcgtcca
     1081 gcgtattgat cgtccacagc ttcaccagca gctgttcctg atgccgctcc gcgccgtgcc
     1141 gccaccgccg gataaagttc atgggacgat gtcctttagc cgacatagcg cgggaatttc
     1201 ttctaccgct gcggcgacgc aggggcggag tctgcagtgc cgcggaaaga gaggatgccc
     1261 acgcttgccg aaattcttgt aatcgagcga ttcaagaatc cagcgcccca ccgcgccgcg
     1321 tctactgtac cgagggctag actttccata ctgtcccaac ggacaggcgt catccggttt
     1381 ttgtagcttt ctctcctcct ttgggtgccc cattctttcg cgcattctgc gaaagggtgg
     1441 gatctgtccg ttcttgctct catgccgttc tccgaacgtt taatcctgaa cctctgccct
     1501 ctttgtcctc tgtgcctctg cgcgataaat ccctccgaag cctctgcgtc ctcgaactct
     1561 ttttgcggta aggtattgcc cgccgttcgg aggcctgaat gcatctctgg caaacgctcg
     1621 ccgagctccg cgccagcggg gcctggtggc tcatcgcgct gcgttattcc atcgcggtgg
     1681 tcgtcggatt ctacgtgctc aaccaggtgc gcaaaccctc gcgctgggtc ggccgcccgt
     1741 tcttgtggat gatgaacctc tcccattcgc cgctcaccga ctggggcttg cagcacatcc
     1801 gcgtcgtaga ggatttccag attctcgatg tcggctgcgg cggcggccgc accattcaaa
     1861 aactcgccgc gctggctccg cagggccaag tctccggcgt ggactatgcc cagggcagcg
     1921 tggccgcttc ccgcgctcgc aatcggcagc tcatcgccgc cggccgcgtc tccatccagg
     1981 aagcttccgt ctcgcgcctg cccttcgccg aaaacacctt cgatctcgtc actgctgtcg
     2041 aaacgcagta ctactggccc gatctgccca acgacctgca ggaaattctc cgcgtcctca
     2101 agcccggcgg cgccctcctc gtcatcgccg aaagctataa aaaggggagc gccaacctgc
     2161 tc
//
LOCUS       DASTPM010000552         2815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1115241, whole genome shotgun sequence.
ACCESSION   DASTPM010000552 DASTPM010000000
VERSION     DASTPM010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115241"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..>2815
                     /locus_tag="VFR42_09785"
     CDS             95..>2815
                     /locus_tag="VFR42_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEU0369490.1"
                     /translation="MKSFLETVRERVVIYDGAMGTEIQERHPSLDDFWGKENCSEVLV
                     LSRPDIIKDIHATYFKAGADVVETNTFGGSRIVLGEFDLADKVHEINKRAAELAREVA
                     QDFSGNGKQRFVAGSIGPGTKLPSLGQIKYDDMFDAYLEQSLGLIDGGVDILLIETCQ
                     DILQTKISVAAAFAAMKQAGKRIPVQAQVTLQESGTMLLGTEIGAAETALEPFECEII
                     GLNCATGPKEMNDAVRYLSHNAPKVISVLPNAGLPENVGGVAHYRLTPDDLAQWHKKF
                     ITEYGVRIVGGCCGTTPVHIKAVSDLCAKLEPARREVRVTPAAASAYSMLPLDLDPKP
                     LIVAEEMNTTTRVEHFKNMVREGKYDDILALAKKLVAEGSHMLDLCTAIVGEDEKGYM
                     SSILERVATRVPAPILVDSTEADVIEEALKRIPGKAIINSINLEDGEKRTAKVLPMAK
                     KYGAAVIALTIDEDGMALTAEKKVAVAKRIFRLASEKYGIRPVDLIFDALTLPISTGQ
                     DDYRTAGIETLNAIKGIKAELPEVKTILGVSNISFGLNTYARRVLNSVFMKEAVDCGL
                     DMAIVNYAKIYPLYKIPEIEVDLARKLIYYDRSGGDPLQVYMQHFTGLDKQPVAEAAP
                     LESLSVEDKLKRCIINGEKSLGDGAQKQTLERILEDALKKYSPLEVINTVLLDGMKTV
                     GELFGARKMQLPSVLDSAGVMKQAVAYLEPKMEKKEGNQKGTLVLATVKGDVHDIGKN
                     LVDIILTNNGYKVINLGIKQPSDSIIAAAKQHNAHAIGLSGLLVKSTLEMKYVIQDLQ
                     AQALEYPVICGGAALTRKYVEDDLRREYKNSVFYADDAFTGLHLMDDLTSTNGAREKR
                     LQEGRTVKEFAKTTAVAGAPTSTSTERSPVVQPAPNIPVPPFYGVRVRKDF"
BASE COUNT          654 a          733 c          905 g          523 t
ORIGIN      
        1 cagaagccaa tcgaggggaa tcagtcgggc ggaaatgaag tggcatttag aataatggca
       61 gcgggaacct gaatcaggtc tcgaccagag gaagatgaaa tcatttcttg aaacagttcg
      121 cgaacgcgtc gttatctatg acggcgcgat gggcacggaa atccaggaac ggcacccgtc
      181 actcgacgat ttctggggca aggagaattg ctctgaagtt ctggtgctga gccggcccga
      241 cattatcaaa gatatccatg ccacgtactt caaggcgggc gccgacgtgg tggaaaccaa
      301 cacctttggc ggcagccgca ttgttctggg cgaatttgat ctggcggaca aggtgcatga
      361 gatcaacaag cgcgctgccg aactggcgcg cgaagtggcg caggattttt ccggcaacgg
      421 aaaacagagg tttgtcgccg ggtcgattgg gccgggaaca aagctgccgt cgctggggca
      481 aatcaagtac gacgacatgt ttgacgcgta tctggaacag tcgttggggc tgattgatgg
      541 cggagtggat attctgctga tcgaaacctg ccaggacatt ctgcaaacca agatctccgt
      601 tgccgcggcg tttgccgcga tgaagcaggc ggggaagcgc attccggtac aagcccaggt
      661 aaccttgcag gaatccggaa ccatgctgct gggcacggaa attggcgcgg ctgaaacggc
      721 cctggagccg tttgagtgcg agattatcgg gctgaactgc gctactgggc caaaagagat
      781 gaatgacgca gtgcgatacc tttcgcacaa cgcgcccaag gtcatttccg tgctgcccaa
      841 cgcgggcctg ccggaaaacg ttggcggcgt ggcgcactat cgccttaccc ccgacgatct
      901 tgcccagtgg cacaaaaaat tcatcactga gtacggcgta cggattgttg gcggatgctg
      961 cgggactacg cctgtgcata tcaaagcggt ctccgatctc tgcgccaaac tggaaccggc
     1021 ccggcgcgag gtgcgggtga cgcccgctgc cgccagcgca tacagcatgc tgccgctgga
     1081 tctcgatccc aaacccctga tcgtggcgga agagatgaac accaccacgc gggtggagca
     1141 tttcaagaac atggtccgcg aaggcaagta tgacgacatt ctggcgctgg cgaagaagct
     1201 ggtggcggaa gggtcgcaca tgctggacct gtgcacggcg attgtgggcg aagacgaaaa
     1261 aggctatatg agctcgatcc tggagcgcgt ggccacgcgc gtgcctgcgc ccatcctggt
     1321 ggactccacc gaagcggacg tgatcgagga ggcgctgaag cggattccgg gcaaggccat
     1381 catcaattcc atcaaccttg aggacggcga gaagcgcacc gccaaggtgc tgccgatggc
     1441 gaagaagtat ggcgcggcgg tgatcgcgct caccattgat gaagacggca tggcgctgac
     1501 ggcggagaag aaagttgcgg tcgccaagcg cattttccgg ctcgcttcag agaaatacgg
     1561 cattcgtccc gtggacctga tctttgacgc cctgacgctg ccaatttcca ccgggcagga
     1621 cgactaccgc accgcgggga tcgagacgct gaacgcgatc aagggaatca aggcagagct
     1681 gcctgaggtg aagacgatcc tgggcgtgag caatatttcg tttggattga acacctatgc
     1741 gcggcgggtg ctgaacagcg tgttcatgaa agaggctgtg gattgcgggc tggatatggc
     1801 gattgtgaac tacgccaaaa tctatccttt atacaagatc ccggaaatcg aagtggacct
     1861 ggcgcgcaag ctgatttact acgaccggtc cggcggtgat ccgttgcagg tttacatgca
     1921 gcatttcacc ggtttggaca aacagcccgt agccgaggcc gcgccgctgg aatcgctttc
     1981 agtggaagac aagctgaagc gttgcatcat taacggcgaa aaatcgctgg gggacggcgc
     2041 gcaaaagcag acgctggagc ggattctgga agacgcgctg aagaagtatt cgcccctgga
     2101 ggtcatcaat accgtactgc tcgacggcat gaagacggtg ggcgagcttt ttggcgcgcg
     2161 caagatgcag ctgccctccg tgctggattc cgccggggtg atgaagcagg cagtcgccta
     2221 tcttgagccg aagatggaga agaaggaagg caaccagaag ggaacgctgg ttctggccac
     2281 ggtaaagggc gacgttcacg atatcggaaa gaaccttgtg gacatcattc ttaccaacaa
     2341 cggctacaag gtgatcaatc tggggatcaa gcagccgtcg gattcgatta ttgcggcagc
     2401 caagcagcac aacgcgcatg ccattgggtt aagcggactg ctggtgaaat cgaccctgga
     2461 gatgaagtat gtgatccagg atttgcaggc ccaggcgctg gagtacccgg tgatctgcgg
     2521 cggagctgcg ctgacccgca agtacgtgga ggacgatctg cggcgggagt acaagaactc
     2581 cgttttctac gcggacgatg cgtttaccgg cctgcacttg atggacgatc tgacctcgac
     2641 caacggagcg cgggagaagc gcctgcagga ggggcgaacg gtgaaggagt tcgccaagac
     2701 cacggcagtc gccggggccc cgacttccac cagcacagaa cggtcgccgg tggtgcagcc
     2761 ggcgccaaat attcctgtgc cgccgtttta cggtgtgcgc gtgcgcaagg atttt
//
LOCUS       DASTPM010000553         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_451962, whole genome shotgun sequence.
ACCESSION   DASTPM010000553 DASTPM010000000
VERSION     DASTPM010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451962"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1419)
                     /locus_tag="VFR42_09790"
     CDS             complement(109..1419)
                     /locus_tag="VFR42_09790"
                     /inference="COORDINATES: protein motif:HMM:NF006775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEU0369491.1"
                     /translation="MAVSPTFNPIATSRAADVIARCQTLARFSEDSASLRRTFLSPPM
                     HDCHGAIAEWLKPLNIIPTIDAAGNLRAVYPGADAAAPRLLLGSHLDTVPNAGAFDGP
                     LGVLLPVALLEQLEGERLPFAIELVAFSEEEGVRFATPFIGSCALVGNYDEEFFNRRD
                     ANGISIRQAIQQFGLDPAQLSAARMSDDALAYIEFHIEQGPVLEALNLPLGAVEAIVG
                     QSRLAVTFTGKSNHAGTTPMNARRDALAAAAEWMVTVESEASRTAGLVATVGSIQAHP
                     GATNVIPGEARLTLDVRHKSDAIRSQSVQRLISLAQQIAARRALTLNFATFLNHPAVA
                     MDSFLTAQIETAMRAAGCPAHRMPSGAGHDAMILASRVPAAMIFLRSPGGISHDPAES
                     VLLEDVACAIDCGGHLLKQLSASPEFLQSVGSPAASQRTDTPRG"
     gene            complement(1419..2264)
                     /gene="pucL"
                     /locus_tag="VFR42_09795"
     CDS             complement(1419..2264)
                     /gene="pucL"
                     /locus_tag="VFR42_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEU0369492.1"
                     /translation="MVKLAENRYGKSRVRLVRVKRHTDRHDLHEWTVQILLHGDFQSC
                     FTDGDNSSILPTDTMKNTVYSLARNSAADCIEDFAKQLVAFLLNGNPQVSAAEAVISE
                     SAWDHLAIGSKPDPTTFVQSSRELRTTTVTQARGAVAQVVSGLKDLVILKTAGSAFTG
                     YIKDALTTLPETRDRLFGTAVGANWTYYSENSAFAQLRCKIRDLLLAAFAKHQSESVQ
                     HTLYAMAQNVLDHAAEVKDIQLTMPNKHCLLVDLSRFGQDNPNEIFVPIDEPHGYIEA
                     RVKRE"
BASE COUNT          506 a          663 c          775 g          465 t
ORIGIN      
        1 atcgcctttt tcatgccggg cagcacggtg cgcacaaagg tatccggagt gagcaatgtg
       61 tggttgcgct gatgcgaact gcgcgtctgg ccgagttggt gcaatgggtt atccacgagg
      121 cgtgtctgtc ctttgcgaag ctgcgggcga gcctacggac tgcaaaaatt ccggcgaagc
      181 agaaagttgt ttgagcaaat ggcctccgca atcgatggcg caggcgacgt cttccagcaa
      241 aacagattcg gcggggtcgt ggctgatgcc gccggggctg cgcaggaaaa tcatcgccgc
      301 gggtacacgc gaggcgagga tcatcgcgtc gtggccggcg ccgctgggca ttcggtgcgc
      361 gggacaaccg gctgcgcgca tggccgtttc gatctgcgca gtgagaaaag aatccatggc
      421 cacggcgggg tgattgagaa acgtcgcaaa attaagcgtg agtgcccgac gcgcggcgat
      481 ttgctgcgcc agagagatca gtcgttgcac ggactgtgat cggatggcat cggatttgtg
      541 gcgaacgtcg agcgtcagac gagcttcgcc ggggataacg ttggtcgccc caggatgagc
      601 ctggatggaa ccaactgtag caacgaggcc ggcggtacgg ctggcctcgg attcaaccgt
      661 gaccatccat tccgcggcgg cggccaaagc atcgcgccgc gcgttcatcg gagttgtccc
      721 ggcgtggtta gacttgcccg taaaagtgac tgccagacgc gactgcccaa cgatcgcttc
      781 gacagcaccc agcggcaggt tgagagcttc gagcacgggc ccctgctcga tgtgaaattc
      841 gatgtaagcg agtgcgtcat cagacatccg cgcagcggat aactgtgcgg gatcgagtcc
      901 aaactgctgg atggcctggc ggatcgagat gccattggcg tcgcgacggt tgaaaaactc
      961 ttcgtcgtag ttgcccacga gcgcgcagct gccgataaag ggcgtcgcaa agcgtacgcc
     1021 ctcttcctcg gaaaacgcga caagttcgat cgcgaaaggc aggcgttcac cctcaagctg
     1081 ctcgagcagc gcgacgggaa gtaaaacgcc cagcggcccg tcaaaggcac cggcgttggg
     1141 caccgtgtcc aggtgagaac ctagcagcag ccgcggcgcc gcagcgtctg cgcccggata
     1201 gaccgcacgg agattgcccg cagcgtcgat cgtgggaatg atgttgagcg gcttgagcca
     1261 ttcggcaatg gcgccgtgac aatcgtgcat cggcggcgaa agaaaagtgc ggcgaagact
     1321 ggcgctatct tcgctgaagc gcgccagcgt ctggcagcgg gcaatgacat ctgccgcgcg
     1381 ggatgttgca atcgggttga aggtcggcga cacggccatc actcgcgctt cacgcgcgcc
     1441 tcgatgtagc catgcggctc atcaatcggc acgaaaattt cattgggatt atcctggcca
     1501 aagcgggaaa gatcgaccag caggcaatgt ttgttgggca tcgtgagctg aatgtctttt
     1561 acttcggccg cgtgatcgag gacgttttgc gccatggcgt agagagtatg ctgcacggat
     1621 tcactttggt gcttggcgaa agccgccaaa agaaggtcgc gaatcttgca gcgaagctga
     1681 gcgaaagcgg agttctcact gtagtacgtc cagttagcgc cgacggccgt gccgaacagc
     1741 cggtcccgcg tttcgggcag cgtcgtcagc gcatccttta tatagccagt gaaggccgag
     1801 ccagcggttt tcagaatcac caggtccttg agaccggaaa ccacctgggc cactgcgccg
     1861 cgcgcctggg ttacggtggt ggtccgtagt tcgcgggacg attgaacaaa cgttgtggga
     1921 tccggcttac taccaattgc aaggtggtcc catgcggatt ccgagatgac agcttcggca
     1981 gcggaaacct gcggattccc gttcagcagg aacgcgacga gttgcttggc aaagtcctcg
     2041 atgcaatccg ccgcggaatt ccgcgccaga gagtacaccg tgtttttcat ggtgtcggta
     2101 ggaagaatgc tgctgttgtc gccgtccgta aagcaggact ggaagtcgcc gtgcaacaaa
     2161 atctgcaccg tccactcatg caagtcgtga cgatcggtgt ggcgcttcac tcgtaccagc
     2221 cgaacgcgcg acttgccata gcgattttcg gcgagtttca ccattcaact tccccgatac
     2281 gtggtgtaac cattggggct cagcaatagc ggaatgtgaa agtgcgattc cccgttgccg
     2341 acgcggaatg cgatctccac gtagggatac agactctgaa tgccttgcga agcgaagtag
     2401 tccgccgtg
//
LOCUS       DASTPM010000554         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_192620, whole genome shotgun sequence.
ACCESSION   DASTPM010000554 DASTPM010000000
VERSION     DASTPM010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192620"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="VFR42_09800"
     CDS             complement(<1..1843)
                     /locus_tag="VFR42_09800"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0369493.1"
                     /translation="MSVLVSQVEAERQRVRAQFDAGATAKETLRQLCELADGTIHQTF
                     GEMMKVHETPNEGLSLLALGGYGRQMLFPYSDLDILFLFENQKSEEEFRPLISEFART
                     MWDLGFRVSSAGRTIDECKRIEEDNAEFHLALLDRRFLAGDQELFDKLDKRILPPSEK
                     QARPFLFSQLHRLTKERLARYGNTIFHLEPNVKEAPGGLRDYQAAFWLRQILGDRPDL
                     RGSSAADEEIAAEAVEFQSAIRCLLHYANARNDNTLTYEMQEEAANRSLAVRDGVKRD
                     AAEWMRLYFRHARTLNRQLLRYLDQKISMPLSLRERFFSAARSVARPEAPPSGAHFAI
                     RNGQFEILNQAALADRAIVYSLFTEAARTGTPLSRDAERMIAYVLSHSELQAINQAIT
                     WDTLKEILAADYPGMALRPMQRLGILMEVLPEFRAIDSLVVRDFYHRYTVDEHSLRTI
                     EHLQALAEPPDARGAQVAALWKAQERRDLLVFALLLHDTGKGMPVENHVQGSLTSLAS
                     AASRLGLTSGEEAEAHFLIEHHLDMSATVQRRDIFDPSTVSGFAATVSTLERLQRLCL
                     LTYADIHAVNPEALTPWKAEMLWQLFVATANHLSRSLDTDRLHASDEA"
     gene            complement(1922..2260)
                     /locus_tag="VFR42_09805"
     CDS             complement(1922..2260)
                     /locus_tag="VFR42_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU0369494.1"
                     /translation="MMKLEAIIQPSRFESVKDALREVGIEGMTVTEVRGHGRQKGHTE
                     VYRGREYTVDLLPKIKLEMVLPDKLVNTVVDTILKTAKTGKIGDGKIFLSRVEEAIRI
                     RNEERGESAL"
     gene            complement(2361..2474)
                     /locus_tag="VFR42_09810"
     CDS             complement(2361..2474)
                     /locus_tag="VFR42_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369495.1"
                     /translation="MILKITDLLTGFRVSEEHEVEGLDITQHGEEAYYLES"
BASE COUNT          491 a          764 c          679 g          541 t
ORIGIN      
        1 aagcttcatc cgaagcatgc agccgatcgg tatcgagact acggctcaga tgatttgccg
       61 tggctacaaa aagctgccag agcatttcgg ccttccacgg agtcaaagct tcgggattga
      121 ccgcgtggat gtccgcgtag gtcagcagac acaggcgctg caagcgttcc agcgtcgaaa
      181 ccgtcgcagc gaatcccgac actgtagatg ggtcgaaaat gtcgcgccgt tgcaccgtag
      241 ccgacatgtc cagatggtgc tcaatcaaaa aatgcgcttc cgcttcttct ccggacgtaa
      301 ggcccaggcg cgaagcggcg ctcgccagcg atgtcaggct gccctgcaca tgattttcca
      361 ccggcatacc cttgccggtg tcgtgcaaca gcagcgcgaa caccagcaag tcgcgtcgct
      421 cttgcgcttt ccataacgca gccacctgcg cgccgcgtgc gtcgggcggt tcggccagcg
      481 cctgcagatg ttcaatggtg cgcagcgaat gctcgtccac ggtgtagcga tgataaaaat
      541 cgcgcaccac caacgaatca attgcgcgaa attccggcag aacttccatc aggatgccca
      601 ggcgttgcat cgggcgcagt gccatgcccg gataatccgc cgccaggatt tcctttaagg
      661 tgtcccaggt aatcgcctga ttgatcgctt gcaactccga atgcgataga acataggcga
      721 tcatgcgttc cgcgtcgcgg ctcaagggtg tgccggtgcg cgcggcttcc gtaaacaggg
      781 aataaacgat cgcccggtcc gccagcgcgg cctggttcag aatttcgaat tggccattgc
      841 gaatcgcaaa atgcgcgcca ctgggaggcg cttcaggccg cgccacgctg cgtgccgcac
      901 taaaaaaccg ctcccgaagc gagagcggca tgctgatttt ctgatccagg tagcgcagta
      961 gctggcggtt caacgtgcgc gcgtggcgaa aatagagccg catccattcc gccgcgtcgc
     1021 gcttcacgcc gtcgcgtaca gccagcgagc ggttcgccgc ttcttcctgc atttcgtaag
     1081 tcagcgtgtt gtcgttgcgc gcgttggcat agtgcaacag acatcggatg gcgctctgaa
     1141 attcgaccgc ttcggcggcg atttcttcat cggccgcaga tgatccgcgc aagtccggcc
     1201 ggtcgcccag aatctgcctc agccagaatg ccgcctgata gtcgcgcaac ccgccgggcg
     1261 cttccttgac gttcggctcc agatgaaaaa tggtgttgcc gtaccgcgcc aggcgctctt
     1321 tggtcagccg gtgcagctgc gaaaataaga acggccgcgc ctgcttttcc gacggaggca
     1381 ggatccgttt gtcgagcttg tcgaacaact cctgatcacc agcgaggaac cttcgatcca
     1441 gtaaggccag gtgaaactct gcgttgtcct cttcaatgcg cttgcactca tcgatcgtgc
     1501 ggcccgcgct gctcacccgg aaacccaggt cccacatcgt gcgggcaaat tcggaaatca
     1561 gcggacgaaa ttcttcctcg gatttctggt tttcgaaaag gaaaaggata tcgagatccg
     1621 agtagggaaa cagcatttgg cggccataac cgccgagcgc cagcagcgaa agcccctcgt
     1681 tcggcgtttc atgcaccttc atcatctcgc cgaacgtctg gtggatagtg ccgtcggcaa
     1741 gctcgcaaag ttgacgcagc gtctccttgg cggtcgcgcc ggcgtcaaat tgcgcacgaa
     1801 cccgctgccg ctccgcttcc acttgcgaaa cgagaacact catcggtttg gctggacctg
     1861 gcatcgttct tgcgtgaggc ttttccggag cagggccggc ttgagcagca ggggacacaa
     1921 tctaaagcgc gctttcgccg cgctcttcgt tgcggatgcg gatcgcctct tctacccgcg
     1981 acaaaaatat tttaccgtcg ccaatctttc ctgtcttggc ggtctttaga atggtgtcca
     2041 caacggtgtt cactaatttg tcgggcagca ccatttcgag cttgatcttc ggcagcaagt
     2101 ccaccgtata ctcgcggccg cggtacacct cggtgtggcc tttctgccgg ccatggccgc
     2161 gcacctccgt caccgtcatg ccttcaatac ccacctcgcg caaggcgtct ttcaccgatt
     2221 caaaccgtga tggctgaatg atggcttcca atttcatcat gggtgttccg ctccgcctcc
     2281 gccgtcccag attatctttg cagccacggg tttatcccgc tctccttatg gttccagcca
     2341 gtcttagatt ccagcccatt tcatgattcc agatagtacg cttcttcgcc gtgttgcgta
     2401 atgtccagcc cttccacttc gtgttcttcg ctcacacgga agccggtcag caggtcagtg
     2461 atcttaagaa tcacg
//
LOCUS       DASTPM010000555         4786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1317039, whole genome shotgun sequence.
ACCESSION   DASTPM010000555 DASTPM010000000
VERSION     DASTPM010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317039"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFR42_09815"
     CDS             <1..1486
                     /locus_tag="VFR42_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEU0369496.1"
                     /translation="ALRYRSLLSQAERIRLSLVALARLRHRLTRENAFHPAIAALDQH
                     RENAARLLKVVAQSLSTGKEPRLEADRLDLGVALAQQLALEDSPAVQTFSNAVLRDMK
                     FQADALGGQLRAVIELARNATPQGEEAFEKREAAQPLWLRFAGRLATLRANLSLDSVI
                     FRHAVRLTLCVAAGDATGRVLHPYRAYWIPMTIVLVLKPEFAVTFSRGVLRIAGTLIG
                     LLLATALFHYLPLGTATEIALIGVFTFLMRWVGPANYGIFGVTISALVVMLISITGLA
                     PKEVIQARAINTVLGGVFALVAYAAWPSWERNRVPELFAALLEAYRKSFGAICAELLN
                     PAPGSGQRRERTRQAARTTRSNLETSRERLAAEPGITQEQLSQVNAMLASSHRFAHAM
                     MALEAGIPAASAQGQAQPAPRPEFRKFAEAVEMTLKLLAAKIRGEKVAERDFPDLRES
                     YLQLASSGDPRLKRYALTNVEADRMTNSLNTLREQVFAWKRLRR"
     gene            1597..1920
                     /locus_tag="VFR42_09820"
     CDS             1597..1920
                     /locus_tag="VFR42_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369497.1"
                     /translation="MGFVSSLAIGGSLFGAQARHKFPEPPTPADPNGSGAKPGDLQAA
                     QRAQLKLNEKEFRESLANLYQRVSELKQDMETTHTADVFSLKVYKQTGEIEHLAKKLR
                     SLAKI"
     gene            complement(1961..2539)
                     /locus_tag="VFR42_09825"
     CDS             complement(1961..2539)
                     /locus_tag="VFR42_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU0369498.1"
                     /translation="MAKKILVLAGDYVEDYELMVATEALMMVGHTVHTVCPGRKAGEF
                     IPTSIHDFEGDQTYTEKKGHNFALTATFDEIKAEDYDALYIPGGRAPEYIRMNLRVLD
                     MVRYFASSCKPLAAICHGPQVLAAAGVLKGKRCNAYPACAPDVTLAGGTYVELPMNES
                     LLDGNLLTGPAWPALHSFLSNLLKALGTKIQA"
     gene            complement(2679..3530)
                     /locus_tag="VFR42_09830"
     CDS             complement(2679..3530)
                     /locus_tag="VFR42_09830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0369499.1"
                     /translation="MVQQYLLASDFDQTLSFNDSGVVLAELIGVPGFHDKVRGLAQIN
                     LVQQGAELSYLILHDPEFRRVRSHHLVEAGKQIRLKHDVALFAKTLENLADGYKFNFR
                     VISAAPQEIIQSALEGIVPPENIHGTKFYYDPGNGEVKAIVRASAGWGKVTVLEEIRA
                     QLGISHDHIVYMGDGSSDLPVMMHVNQYDGLTIAVSEAEFITRIARRTVLSDNAMSVL
                     IPVLEELLRWDSSKIRRYFATQGLMLKEWGKTRTDQLTIQESPSDSPELAEAQTTVAA
                     TAPVITA"
     gene            complement(3815..>4786)
                     /locus_tag="VFR42_09835"
     CDS             complement(3815..>4786)
                     /locus_tag="VFR42_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369500.1"
                     /translation="EEGLVVVGNPNLKAESPGVGTLLDGNPANNFLQRALAFNPGGIL
                     TGARRITIAGNYAYVLTGKNLVVVDLENPLAPQVTATIGAPALDEPRGIAIQFRYAFI
                     VDRFGLKVLDVTDLAHPKPVEKAKVAVDDARNIYVARTYAYIAAGKNGLGIVDVENPE
                     APRFDQFFTANNSMNDVNDVKIGMVAASAFAFLADGRNGLRILQIVSPWDDPAHFSGF
                     SPRPLAKLIATAHTKGPALAISKGIDRDRAVDESGNQISVFGRRGARPLNRREAQRLY
                     LRDGKLYTVADDGEGSQADKNTDLIGPGFWQGLGKAWNWFSPLPAAR"
BASE COUNT          984 a         1251 c         1548 g         1003 t
ORIGIN      
        1 ggcgctgcgg tatcggtcgt tgctatcgca ggcggagcga atccggctga gcctagtggc
       61 gctggcgcgg ctgcggcacc ggttgacgcg ggaaaatgcg tttcatccgg cgatcgcggc
      121 gctcgaccag catcgggaga atgcggcgcg cctgctgaag gtagtggcgc agagcttgag
      181 caccggcaaa gaacctcggt tggaggcgga ccggttggat ttgggcgtgg cgctggcgca
      241 acagctggcc ttggaggatt ccccggcggt gcaaacattt tcgaatgcgg tgctgcgcga
      301 catgaaattt caggctgacg cactgggcgg acagctgcgc gcagtgatcg aactggcgag
      361 gaatgcgacg ccacagggtg aagaagcgtt tgagaaacgc gaggcggcgc agccgctgtg
      421 gctgcggttt gcgggaagac tagcgacgct tcgggcgaat ttgagcctgg attcggtcat
      481 tttccggcac gcggtgcggt tgacgctgtg tgttgcagcg ggcgacgcga caggccgcgt
      541 actgcacccc tatcgcgcct actggattcc gatgaccatt gtgctggtgc tgaagccgga
      601 gtttgcggtg acgttcagcc ggggagtgtt gcggattgcg ggcacgctga tcgggctgtt
      661 gctggcgacg gcgttatttc actatttgcc gcttggtacg gcgaccgaga tcgctctgat
      721 cggggtgttt acattcttga tgcgctgggt gggaccagca aactacggaa tttttggagt
      781 gaccatcagc gcgttggtgg tgatgctgat atcgattacc ggcctggcac cgaaagaggt
      841 gatccaggcg cgcgcgatca acaccgtgtt gggcggggtt tttgccttgg tggcctatgc
      901 ggcgtggccg tcgtgggaac gcaaccgtgt gccggaattg tttgctgcgc tgctggaagc
      961 gtaccggaag tcgttcggcg cgatttgcgc cgagctactg aatccagcgc caggctcggg
     1021 ccagcggcga gaacgtacga ggcaagcggc gcgaacgaca cgatcgaatc tggaaacttc
     1081 gcgggagcgc ttggccgccg agccgggaat tacgcaggag caactcagcc aggtaaacgc
     1141 gatgctggcg tcatcgcacc ggttcgcgca cgcgatgatg gcactggagg cggggattcc
     1201 ggcggcatcc gcacaggggc aggctcaacc ggcgccgcgg ccagagtttc gtaaatttgc
     1261 ggaagcggtg gagatgacat tgaaactgtt ggcggcgaaa attcgcgggg agaaagttgc
     1321 ggaacgtgat tttccggact tgcgagaaag ctacttgcaa ctggcgagtt cgggcgaccc
     1381 gcgattgaaa cgttatgcgc tcaccaatgt ggaagccgac cgcatgacga atagtttgaa
     1441 tacgttgcgc gagcaggtat ttgcatggaa gcgactcagg cgttgacagc ggcgaaaagt
     1501 tgtaggaaaa cgggcatatt cgggcagccg gtttcggagg gggtggcggc catgagttca
     1561 acttcttttc ctcttaggcg tgatttcctc agatatgtgg ggtttgtaag ctctctagca
     1621 ataggtggca gcttgttcgg ggcacaggcg aggcataaat tcccggagcc gccgacacca
     1681 gccgatccga acggcagcgg cgcaaagccg ggcgatctac aggcggcgca gcgggcccaa
     1741 ctgaaattga acgagaaaga gttccgcgag tcgctggcga acctctatca acgggtcagc
     1801 gaactaaagc aggacatgga gaccacgcac accgcggatg ttttttcgct gaaggtttat
     1861 aagcaaacag gcgagatcga gcacctggcg aagaagctgc gcagcctggc gaaaatctga
     1921 acccgccaag cgcaggaatg agtgggcgca cttggcccgc tcaggcctgg atttttgtcc
     1981 caagcgcttt cagcaaattg ctcaggaaag aatgcagagc cggccacgca ggaccggtga
     2041 gcaggtttcc gtccagcaac gactcgttca tcggaagttc aacataggta ccgcccgcca
     2101 gcgtgacgtc cggagcgcaa gcgggataag cgttgcacct cttgcctttt aaaacgccgg
     2161 ccgcggcgag cacttgcggg ccatgacaga ttgccgccag cggcttgcag gatgacgcga
     2221 agtagcgaac catgtccagc acgcgcaggt tcatgcggat gtattcgggc gcgcggccgc
     2281 cgggaatgta cagagcatcg tagtcctccg ctttaatctc gtcgaaggtg gctgtgagcg
     2341 caaagttgtg gccttttttt tccgtatatg tctgatcgcc ttcgaagtcg tgaatggaag
     2401 ttggaataaa ttcaccggct ttgcgtcccg gacaaacggt gtgcacggtg tgtccgacca
     2461 tcatcaacgc ttcggtggcc accatcaatt cgtagtcttc aacgtagtcg ccggccagca
     2521 ctaaaatctt tttggccatt taacctcccg tgtgactgac gcgcagtagt gtagcgcggc
     2581 taacccgaaa tagaaaaacg gggcaataaa aatgggcgct gcattcatcc ttttttggaa
     2641 ggaagaaagc cgcgcccgaa accgtatgga gcgctgcgct aggcggtgat gaccggcgca
     2701 gttgcagcaa cggtggtttg tgcctcagct agctccggcg agtccgaggg cgactcctgg
     2761 atggtcaact ggtcagtacg tgttttgccc cattctttga gcatcaggcc ctgagtggcg
     2821 aagtaacgcc ggattttcga agagtcccag cgcaacagtt cttccagaac tgggatcagt
     2881 acgctcatgg cgttgtcgct gagcacggtg cggcgggcga tgcgggttat gaattccgct
     2941 tcgctgaccg ctatggtcag tccatcgtac tggttgacgt gcatcatgac gggcaagtcg
     3001 gaactgccgt cgcccatgta aacaatgtgg tcgtggctga tgccgagttg cgcgcgaatc
     3061 tcttcgagta cggtcacttt gccccagccc gccgaagcgc gaacgatggc tttaacttct
     3121 ccgttgcccg gatcgtagta aaacttcgtg ccgtgaatat tttccggcgg gacaatgcct
     3181 tcaagcgccg actgaatgat ttcctgcggc gctgcggaga tgacgcgaaa gttgaatttg
     3241 tagccgtccg cgagattttc cagagttttc gcgaacagcg cgacgtcatg cttcaggcga
     3301 atctgtttgc ctgcttcgac caggtggtgg ctccgcacgc ggcggaactc cggatcgtgc
     3361 aggatcaggt acgagagctc ggcgccctgc tgtaccaggt taatttgggc caatccccgg
     3421 actttgtcat ggaagcctgg cacgccgata agctcggcaa gcacgactcc cgaatcattg
     3481 aaactgagcg tctggtcgaa atcgctggcc agaagatact gctgcaccat taggttgcaa
     3541 cccctttctt ggttcgcccc gattgccgga gctgcgccgc ccgctggcgg ctgagtgccc
     3601 cccgtgtaca cattacctgc gttcacggcc tgggtggcac gaattatttt gatgggtgct
     3661 atgcacagaa acgtgaacag aatgttgtca gttcattaga tgcatcatac cgctaaaagg
     3721 ttgaagggac gaagcttagt agagttaacg ctgaaggatg ggcgtggaga agcgaaacct
     3781 gcacgaagga acagtgcgaa taaaaagcga atcatcatct cgcggctggg agtgggctaa
     3841 accaattcca ggctttacca agcccctgcc agaaacccgg accaataagg tccgtgtttt
     3901 tgtcagcttg cgacccttcc ccgtcatccg cgaccgtata gagcttgccg tctcgcaggt
     3961 acagccgctg cgcttccctg cggttcaagg gccgcgcgcc gcggcgcccg aagaccgaaa
     4021 tctggttgcc ggattcgtcc acggcgcggt ctcgatcaat gcctttggag atcgctaacg
     4081 ccggaccttt tgtgtgtgca gtggcgatga gttttgcaag gggacgcggg ctgaagcccg
     4141 aaaaatgcgc ggggtcgtcc cacggcgaga cgatttgcag gatgcggagg ccgttacgac
     4201 catcggccag gaaagcgaag gcgctggccg cgaccatgcc gatcttgacg tcgttgacgt
     4261 cgttcatgga gttgttggca gtaaaaaatt gatcgaagcg cggcgcttcg ggattttcca
     4321 cgtcaacgat acccaggcca tttttgccgg cggcgatgta tgcgtaggtg cgggcgacgt
     4381 aaatgtttcg ggcatcgtcg actgcaactt tggccttttc caccggctta ggatgcgcga
     4441 ggtcggttac gtcgagaact ttgaggccga agcggtccac gatgaacgcg tagcgaaatt
     4501 gaatggcgat gccgcgtggc tcatcgagcg caggcgcacc gatggtggcg gtgacttgcg
     4561 gcgcgagggg attttcgaga tcgaccacga cgagattttt tccggttaga acataggcgt
     4621 aatttccggc gatggtgatg cggcgggcgc ctgtgagaat tccaccggga ttgaaggcca
     4681 gggcccgttg caggaaattg ttggcgggat tgccatcgag caaggtaccg acaccggggc
     4741 tttcggcttt gagattcgga ttgccgacga ctacgaggcc ttcctc
//
LOCUS       DASTPM010000556         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_336858, whole genome shotgun sequence.
ACCESSION   DASTPM010000556 DASTPM010000000
VERSION     DASTPM010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336858"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..800
                     /locus_tag="VFR42_09840"
     CDS             378..800
                     /locus_tag="VFR42_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369501.1"
                     /translation="MNRIRTALFGLGLFFAISGVQAQENGVKANVPFDFVVGNQVLPA
                     GEYLVTAQGSGNQAILIRSTDRKSAAMTTTFSCTSASPSKDTKLVFHTLGGRYFLSQV
                     WAEGYSQGRELRKSSAEIEMAKNQQDAGEFVLAARSAR"
     gene            complement(948..1478)
                     /locus_tag="VFR42_09845"
     CDS             complement(948..1478)
                     /locus_tag="VFR42_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369502.1"
                     /translation="MNDAEARQKRIDEENISTADLAGARTDAATDREVERARRGDIID
                     VQPDNRNVAAVEVQKTEVTAQAVPVEQVGGSAAAAAPRSNEPERGPLFSAEEAVNLRS
                     RWDAIQVGFVDEPRRSVQEADSLVATAMKRLAEQFAAERSTLEGQWDRGGDVSTEDLR
                     IALRRYRSFFGRLLSV"
     gene            complement(1530..2084)
                     /locus_tag="VFR42_09850"
     CDS             complement(1530..2084)
                     /locus_tag="VFR42_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369503.1"
                     /translation="MTQNQVIAIIVAVLAVVAVVLFYLKTQRSRRLRERFGPEYDRAV
                     SEAGSLRAESRLEKLERRVNKYNIRALEPSTRDRYVQEWRLVQARFVDDPNNAVGAAD
                     RLLGEVMAARGYPVADFDQQADDLSVNHPLVIEHYRAGHTIAVRHAQGHATTEELRQA
                     LIHYRALFDELVDERTRAAVATRY"
     gene            2246..>3267
                     /locus_tag="VFR42_09855"
     CDS             2246..>3267
                     /locus_tag="VFR42_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0369504.1"
                     /translation="MADPAISPPEAAPPPALDHSRRQLEALLDVSEIIAQHRDLNTLF
                     HELAARLHSVVESDFLTLVLHDPEKNVMRLHVLESRVETEKTIGFEHPVDASPSGWVW
                     QTQQPFIVEDVLTETRFAEFLGKLKSEGVRSFAALPLTTAQHRLGAMGFGRLRPQAIS
                     DSDIRFMQRVAAQVAVAVDNALNFESSQAYQRQLAEQRDRLQVLLDINNVLVTSRELS
                     ELFRGIVSALSRVLRHDYTSLALLDSATNLLKIHALDFDGADVLTPGKEITVALDQSP
                     AGRCIVTNKPAIFHGAELDQFPAEIVHILRSKGILSACCVPLMTKGRAFGTLNLASRR
                     LDAFSDA"
BASE COUNT          653 a          911 c          983 g          720 t
ORIGIN      
        1 accggcgacg ccaagtaacc gaggattcat gcgtcaactc tctggctgtg gtttatcaca
       61 ctttgcggtt ttggggagag cttggtgagc gattaaccga tcggcgggtt ttcgctaacc
      121 gaatggttag tggaactcaa ccacggagtg tttgcgagag ggcagtagag ggtcttcgac
      181 gttgtaagtg gcgtgctttc agcggaaggc ttgccgcgcc gagaaacggc ggcggttttc
      241 atgcttggca tgcgaactgc ataccttgat tgcgtccctg gttatcaaag ccactgacat
      301 tagaacgacg aaagcagcca agcgccagcc agaacgtccg gcccggccta ctcttcggcg
      361 tttttagagg aggttttatg aatcgcatcc gcacagctct ttttggttta ggattgtttt
      421 tcgcgatttc tggtgtgcag gcgcaggaga acggcgtaaa ggccaacgtt cccttcgact
      481 tcgtggttgg gaatcaagtt cttcccgccg gagaatacct ggtgaccgcg caaggatccg
      541 gcaaccaggc aatcttgatt cgttccacgg accgcaagag cgcggcaatg acaactacat
      601 tcagctgcac atccgccagc ccttcgaagg acacgaagtt ggtgttccac acgctcggcg
      661 gccgctattt cttgtcgcag gtttgggcgg agggttacag ccagggccgg gagctgcgca
      721 agtccagcgc cgaaatcgaa atggcgaaga accagcagga cgcgggcgag tttgttctgg
      781 cagccagatc tgcccgttga aaagtacggc agtagttagg gtgatgccca ggccttcgga
      841 ataacggcac gctccacaag cgcatggcgc atctggagcg tgccttttct tttttggcgt
      901 cgcgcgaagc aagccgctga agaaaagtgg tatgcgcgga gcgttcgcta caccgacagc
      961 aagcgcccga aaaatgaccg gtagcgccgc agcgcgatgc gcagatcttc ggtagagacg
     1021 tctccaccgc gatcccactg cccttcaagg gtggagcgtt ctgcagcgaa ctgttcggcg
     1081 agacgcttca tggccgtggc taccaggctg tccgcttcct gcactgagcg gcgcggctca
     1141 tccacgaagc caacttgaat cgcgtcccaa cgtgaacgga gattgacagc ttcttctgca
     1201 gaaaatagcg gaccacgttc gggttcgttg gatctcggcg cggccgcggc ggcactgccc
     1261 ccgacctgtt ctacgggaac cgcctgggcg gtaacctcgg ttttttgcac ctcaactgcc
     1321 gcgacattgc gattgtcggg ctgcacatcg atgatgtcgc cacgtcgtgc acgttctact
     1381 tcccggtcgg tagccgcgtc ggtgcgtgct ccagcgagat cggcggtaga aatattctct
     1441 tcgtcgattc gtttttgtct tgcttcagcg tcgttcaaag taccctccta gaatctgttg
     1501 tcgatgtcgc gtcttgaacc ccaaaacgct cagtagcgag tagcaactgc ggcgcgcgta
     1561 cgttcgtcaa ctaattcgtc aaacagcgcg cggtagtgaa tcagggcctg ccgcaattcc
     1621 tcggttgtgg cgtggccttg cgcgtgacgc acagcgatgg tgtgcccggc gcggtagtgc
     1681 tcaatgacca aaggatgatt cacggaaaga tcgtccgctt gttggtcgaa gtccgcgacc
     1741 ggataaccac gagccgccat gacctcgcca agcaaccggt ctgcggcgcc aactgcgttg
     1801 ttcggatcgt ctacaaatct cgcttgcacc agtcgccact cttgcacgta ccgatcgcgt
     1861 gtggaaggtt cgagtgcgcg gatgttgtac ttattgacgc gccgttcgag tttctccagc
     1921 cgcgattccg cgcggagact gccggcttcg cttaccgcgc ggtcgtattc gggcccgaag
     1981 cgttcgcgca gcctcctaga cctttgtgtc tttaggtaga aaaggacgac ggctacaacg
     2041 gcgagaacag caacgataat cgcgataacc tgattttgcg tcatatcgaa taagctctcc
     2101 gctaacagtt gatagaccct gggtggaaat cgtcctatcc ggttatcgct gtaacgcgcc
     2161 ggatttatct tcgtatgtgc aataagccaa ggacgtcctt tttccgtcgg ttgagcgggt
     2221 atgttggcgg tgctattctg ccgatatggc ggatcctgcc atttctccgc cggaggcagc
     2281 gccgcctccg gcgctcgatc attcccggcg tcaactggaa gcactgctgg atgtcagcga
     2341 aatcatcgcg cagcaccgcg acctgaacac actgttccac gaactcgccg cacggctgca
     2401 ttcggtggtg gagtcagatt tcttgacgct ggtgctgcac gatccggaaa aaaatgtgat
     2461 gcggctgcac gtgctggaaa gtcgcgtgga aaccgaaaaa acgattggat tcgagcatcc
     2521 ggtggacgcc agcccatccg gctgggtatg gcagacacag cagcctttca tcgtggaaga
     2581 cgtgctgacg gaaacgcgct tcgcggagtt tctggggaag ttgaaaagtg aaggggttcg
     2641 ttcgtttgcg gcgctacccc tgaccacggc gcaacatcga ttgggcgcga tgggattcgg
     2701 acgtttgcgg ccgcaggcga tttcggattc agacattcga ttcatgcagc gcgtggcggc
     2761 gcaagttgcg gtggcagtgg acaacgcgct gaattttgag agttcgcagg cctatcaacg
     2821 gcaactggcc gaacagcgcg accgcctgca agtgctgctg gacatcaaca acgtactggt
     2881 gaccagccgg gagctgagcg agttatttcg cggcattgtt tcggcgctgt cgcgcgtgct
     2941 gcgtcacgac tataccagcc tggcgctact ggattccgcc accaacctgc tgaaaattca
     3001 tgcgctggat tttgacggcg cggatgtgct cacgccgggc aaggaaatta ccgttgcgct
     3061 cgaccagtcg ccggcgggcc gctgcatcgt gaccaacaag ccggccattt ttcacggcgc
     3121 ggaactggac cagtttccgg cggaaattgt gcacattctg cggagcaagg gaatactgtc
     3181 cgcgtgctgc gtgccgctga tgacgaaagg ccgcgcgttt ggcacgctga atctggctag
     3241 ccggcgccta gatgcttttt ccgacgc
//
LOCUS       DASTPM010000557         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_740575, whole genome shotgun sequence.
ACCESSION   DASTPM010000557 DASTPM010000000
VERSION     DASTPM010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740575"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VFR42_09860"
     CDS             <1..1285
                     /locus_tag="VFR42_09860"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0369505.1"
                     /translation="YYKLPNGLKVVVSEDHTAPVVTVAVYYNVGFRLEPKGRTGFAHL
                     FEHMMFQGSENAAKMQHIKLINSSGGVLNGSTMYDVTNYYEAVPSNALERVLWLEADR
                     MRALKVDDENLCNQRDVVKEEVRVNVMNQPYGGFPWLDMPPVAFRNWANAHNFYGDFV
                     DLDAASLADVQTFFRTYYVPNNAVLLILGDVNSADGFALAEKHFANIPAGPAPKFTDP
                     SEPQQSEERRGNVLEKFGTLPAMAIGYVLPQRRTADWCAMALLDQALHGGRAGRIYRE
                     LVLEKQLAVEADGGIDDIFGYNGPSQMTTRILHKPEITSETTLAAFDAIIQEVQEKGI
                     TDSELEQLKVKWRSDYFATLEGGRGGYMPKYGLMHLLACFTLFDGQPQLVNSILDGFL
                     AVTKEQVQSVARTYLKQENRAIVFRAPAGTSQEAA"
     gene            1285..>2294
                     /locus_tag="VFR42_09865"
     CDS             1285..>2294
                     /locus_tag="VFR42_09865"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0369506.1"
                     /translation="MATQAPRTASTVPALAPEREVRWPHRTKAKLANGLELILVESHT
                     IPKFHGELFFRSGNAAAADRAISLAEITAAMVRTGTAKSSSREIEERLRGLGADLSVS
                     AGQDTSAISFAGLSDRAEPLLQLVNELAREASFPATEFERERRQKLEEVKLERTQPGF
                     LAGERLRGVLFDKHPYSLVSPTEEQVAAYSVEQLQSLYRDFYTPENAILLLVGDFEPA
                     KVRDAVEKVFGNWSGKKPILQESPAPATPIGRRVYLVHVPGAVQTQILCGCHAITRKH
                     PDWVKLGLTNSLYGGAFNSRLVMNIREEKGYTYSPRSAVNPLRQYGYFSVSAAVRNEV
                     VAA"
BASE COUNT          486 a          753 c          592 g          463 t
ORIGIN      
        1 ctattacaaa ctgccgaacg gactgaaggt cgtcgtctcc gaagatcaca ccgcgccggt
       61 cgtcacagtc gccgtctatt acaacgtcgg cttccgcctc gaaccgaaag gccgcaccgg
      121 cttcgcccac ctgttcgagc acatgatgtt tcagggctcc gaaaacgccg ccaagatgca
      181 gcacatcaag cttatcaact ccagcggcgg cgtactcaac ggctccacga tgtacgatgt
      241 caccaattac tacgaggcgg tcccttcgaa tgctctcgaa cgcgttcttt ggcttgaagc
      301 cgaccgcatg cgcgctctca aggtggacga cgaaaatctc tgcaatcagc gcgacgtggt
      361 aaaggaagaa gttcgcgtca acgtgatgaa ccaaccctac ggtggcttcc cctggctgga
      421 catgcctccc gtcgccttcc gcaattgggc caacgcccac aatttttacg gcgactttgt
      481 cgatctcgat gccgccagcc tcgccgatgt gcagactttc tttcgcacct actacgttcc
      541 caacaacgca gtactgctga tactcggcga cgtgaactcc gcggacggct tcgcgctcgc
      601 cgaaaaacat ttcgcgaaca ttcccgctgg accggcaccc aagttcaccg atccgtcgga
      661 accgcagcag tccgaggaac gccgtggcaa cgtcttggaa aaattcggca ctctccccgc
      721 catggccatt ggttacgtac ttcctcagcg ccgcaccgcc gattggtgcg ccatggcgct
      781 cctcgatcag gcgctgcacg gtggacgcgc tggccgtatc tacagggagc tcgtcctcga
      841 aaagcaactg gctgttgaag ccgatggcgg cattgacgac atcttcggct acaacggtcc
      901 ctcgcaaatg accacacgca tcctgcacaa accggaaatc accagcgaaa cgaccctcgc
      961 cgcgttcgat gccatcatcc aggaagtcca ggagaaggga attaccgact ccgaattgga
     1021 gcaactcaag gtcaagtggc gctcggatta tttcgccacg ctggaaggcg gtcgcggtgg
     1081 ctacatgccg aaatatggcc tgatgcacct gctcgcctgc ttcactttgt tcgacggcca
     1141 accgcagctc gtgaattcga tactcgacgg tttcttggcc gtcacaaaag aacaagtgca
     1201 aagcgtcgcg cgcacgtacc tcaaacagga aaaccgtgcc atcgtcttcc gcgcgcctgc
     1261 cggcacttct caggaggctg cgtaatggcc acccaggccc cgcgtaccgc atcgactgtt
     1321 cctgccctgg ctcccgagcg cgaagtccgt tggccgcatc gcaccaaagc caagctcgcc
     1381 aacggcctcg aactcatcct cgttgagtct cacaccatcc ccaagtttca cggcgaactt
     1441 ttcttccgct ccggcaatgc cgctgctgcc gatcgagcaa tctccctcgc cgagatcacc
     1501 gccgctatgg tgcgcacggg tacggcaaag agttctagcc gcgaaatcga agagcgcttg
     1561 cgcggtctcg gcgccgatct ttctgttagt gccggccagg ataccagcgc catctccttt
     1621 gccggcctca gcgaccgcgc cgagccgctc ctgcaactcg tcaatgagtt ggcccgcgaa
     1681 gcttcttttc ccgcaacaga attcgaacgc gaacgtcgcc agaaactcga agaagtaaag
     1741 ctcgagcgga ctcaacccgg ctttctggct ggcgaacgtc tgcgaggagt cctttttgac
     1801 aagcatccat actccctggt ttcgccgacc gaggagcaag ttgccgcata ctccgtcgag
     1861 cagctgcagt cgctctatcg cgacttctac acgccggaaa atgccattct cctgctcgtc
     1921 ggagattttg aaccggcgaa agtgcgcgac gccgtcgaaa aagtttttgg gaattggtcc
     1981 ggaaagaagc ccatcctgca ggagtctccg gcgcctgcaa cgcccatcgg tcggcgcgtc
     2041 tatctcgttc acgtccctgg cgcggttcaa acgcaaattc tctgcggatg ccacgccatc
     2101 actcgtaaac atcccgattg ggtcaagctc ggtctgacca atagcctgta tggcggcgcc
     2161 ttcaattcgc gactggtcat gaacattcgc gaagaaaagg gttacaccta cagcccgcgt
     2221 agcgccgtga atccgcttcg ccaatatggc tatttttctg tctccgcagc cgttcgcaac
     2281 gaagttgtcg cggc
//
LOCUS       DASTPM010000558         2985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_768377, whole genome shotgun sequence.
ACCESSION   DASTPM010000558 DASTPM010000000
VERSION     DASTPM010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768377"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..604)
                     /locus_tag="VFR42_09870"
     CDS             complement(200..604)
                     /locus_tag="VFR42_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369507.1"
                     /translation="MFSAMLLTISIVALAQFAAYYWRSILAGVAAQPISAEVLSAASV
                     DRGILSGRDFQVVAKLHELTPNLQKRSSSIGLVRAYFHVIHTIGQLAAGRMAGLADWA
                     ERERVLCVRYAAVQVDRRLQSNLALAAAIRSC"
     gene            754..1266
                     /locus_tag="VFR42_09875"
     CDS             754..1266
                     /locus_tag="VFR42_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0369508.1"
                     /translation="MVQSAGTRFARFSALSLLFVFAFGHLYLRAQSTPTLAKTQVSPA
                     DYSHEIRAVLTEQQAAWNRGDIPAFLEGYWNSPDLTFAGSDGIVRGYDGLLERYRKTY
                     PDKARMGELEFAGLEIRPLGADSALVVGHWHLKRTVGDAGGVFSLVFHRFPVGWRIIH
                     DHTSAQKLTP"
     gene            1567..2097
                     /locus_tag="VFR42_09880"
     CDS             1567..2097
                     /locus_tag="VFR42_09880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEU0369509.1"
                     /translation="MIAAALVFSMPSKADTVVFTLTGGAVGGSVTFSLPDTFTPTAGT
                     GSGPFLFANVVATPSPSNPVFGSSQFTLPNVELGLNATGFWSFGSNGVPGFPGTTGNF
                     LGIFAPGLYTVNADGTITLNEALGTLTVINNVGAPLTLTEAVIPSPVATPEPATLALL
                     GIGGLALAGLHRRKAA"
     gene            complement(2178..>2985)
                     /locus_tag="VFR42_09885"
     CDS             complement(2178..>2985)
                     /locus_tag="VFR42_09885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU0369510.1"
                     /translation="EDSLEDAQVRKLDLICRKLQLQPGDQVLEIGCGWGAFAIYAARH
                     YGVHVTALTLSLAQYDFVTQLAAATLEPGSVQFLLQDYRTIDHRAMTGKFDKIVSIEM
                     FEAVGLDRYDEFFSACDRLLTRGGAMLLQLITLPDREFATYRKRVDWIQTYIFPGSEL
                     ASIAEIHRALARSTRMFLVQMENFGLHYARTLARWRERFFANLKSVERLAFDARFQRM
                     WEFYLAWCEGAFLERYINVAQLVLAKNGTQRPLIGDPLTAQTPLARKASA"
BASE COUNT          649 a          852 c          840 g          644 t
ORIGIN      
        1 aggcttgacc agctgaatca tgaagacgaa cataaagaac gtgatgacga acgatgcgta
       61 aatcgtccaa atttccgcaa cagttttttc tctggaggcc attttcatgt acccactttg
      121 cgcggcggta ttgtgccgat cccgaactca gcacgcaacc tgacttctaa cccgcaggag
      181 caaagcgtaa gagggaaaac tagcaggaac ggatggcggc ggcgagggcc aggttcgatt
      241 gcagccggcg atccacttgc actgctgcgt aacgcacgca gagcacccgt tcgcgttccg
      301 cccagtccgc cagaccagcc atgcggccgg cagccagctg gcctatcgtg tgaatcacgt
      361 gaaagtaggc gcgcaccagg ccgatgctgg aggatctctt ctgcaggttg ggggtcaact
      421 catgcagctt ggcgacgacc tgaaagtccc gcccactcag tatcccgcga tcaacgctcg
      481 ccgccgacag cacctcggcg gagatcggct gcgccgctac tccggcgagg atggagcgcc
      541 agtagtaggc ggcgaactgc gccagcgcca caatcgagat tgtaagcagc atcgcggaga
      601 acatttctcg ctccgggaac tattctgctg cccgtctttg cgggctgcca tagcgcggca
      661 gtactagcgc gagtagtcct ttccggatta acttattgcc taccgcgcac ggccctggcg
      721 gtccgttatg aggcactaaa aggagacatc gtcatggtcc aatccgcagg cacccggttc
      781 gcccggtttt ctgccctttc acttctcttt gtcttcgcct tcgggcacct gtacctcaga
      841 gcgcagtcca ctccaaccct cgcaaaaact caggtttccc cggccgacta ctcccatgaa
      901 attcgggcag tgttaaccga acagcaagcc gcctggaatc gcggcgatat tccggcattc
      961 ctcgagggtt attggaattc gccggaccta acctttgccg gaagcgatgg catcgttcgc
     1021 ggctacgacg gactattgga gcgataccgc aaaacctatc ctgacaaggc caggatgggc
     1081 gaactggaat tcgcaggcct cgaaatccgc cccctcggag ccgattcggc actggttgta
     1141 ggccactggc acttgaaacg gacggtgggg gatgccggag gtgtattttc tctggttttt
     1201 cataggtttc cggtgggatg gcggatcatt cacgatcaca ccagtgcaca aaagctgacg
     1261 ccttagtgct aagccgccag attctcgtag tatgctgaaa acaaggtgct tacgctataa
     1321 gaccctgtgg aaaaaggaca gttaactaag gaaacgagtt tcccaattgt acccgtccac
     1381 aaaaactaaa acgctgaaaa taaaggacat aagaaccctg ccgttttggc aatttgcatg
     1441 cttctaccta cgccgcaggt tgaaagtgta ccgatttggc cagttcactc cgcaggctga
     1501 cgatcggact ttttctggac caactagagg acacctatgc gtaagcatct tctgattgcg
     1561 gggattttga tcgcggccgc cctggttttt tccatgccgt cgaaggctga tacggttgta
     1621 ttcacgctga ctggcggcgc ggtcggcggc tcggtcacct tctccctgcc agatactttc
     1681 actcccacag caggcaccgg ctccggacct ttccttttcg ccaacgttgt tgccacaccc
     1741 agtccgagca atcctgtttt tggttcatcg cagtttacac tccctaacgt cgagctcggg
     1801 ctaaacgcga ctggtttctg gtcgtttggc agcaacggag tcccgggctt tcctggcacc
     1861 acaggtaact tcctgggaat ctttgcgcca ggcctgtaca cggtaaacgc ggacggcacg
     1921 atcacgctga acgaggcgct cggcacattg acagtgatca ataatgtggg cgcgccgctg
     1981 accctgaccg aggcggtaat ccccagcccc gtcgccacgc ctgagccggc aacgctggcg
     2041 ctgctcggaa ttggcgggct ggctttggct ggtcttcatc ggcgcaaagc agcctaacta
     2101 agaaaaaact cttctttcgg gcgcctgaat ttttcaggcg cctttttctt tttcctgtag
     2161 cgtaaagtcc agccggatta ggcgctggct ttccgagcga gcggagtttg ggctgtgagg
     2221 ggatcgccga tgagagggcg ttgcgttcca tttttcgcca agaccagttg cgcgacgttt
     2281 atgtagcgct cgagaaaggc gccttcgcac caggctagat aaaattccca catacgctga
     2341 aagcgcgcgt caaaggcgag gcgttccacg gatttcagat tggcgaaaaa gcgctcgcgc
     2401 cagcgagcga gtgtgcgcgc atagtgcaat ccaaaatttt ccatttgcac cagaaacatc
     2461 cgtgtggagc gagcgagagc ccggtgaatt tccgcaatgg acgcgagttc gctgcccggg
     2521 aaaatatacg tttgaatcca gtccacacgc ttgcggtaag tggcgaattc gcgatccggc
     2581 aaagtgatca actgaagcag catggcgccg ccgcgagtga gcaggcggtc gcaggcgctg
     2641 aagaattcgt cgtagcggtc gagaccgacg gcttcaaaca tttcgatgct gacaattttg
     2701 tcgaacttgc cagtcatcgc tcgatggtca atcgtgcgat agtcctgcaa aaggaattgc
     2761 accgatcccg gttcgagagt cgcggcggcg agctgggtga cgaaatcata ctgtgcgagg
     2821 ctaagggtca gcgcggtgac gtgtactccg taatggcgcg ctgcgtaaat ggcgaacgcg
     2881 ccccagccgc aaccgatttc gagaacctga tcgccgggct gcagttgcag tttccggcag
     2941 atgagatcga gcttgcggac ctgcgcgtct tccaaagaat cttcc
//
LOCUS       DASTPM010000559         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_106148, whole genome shotgun sequence.
ACCESSION   DASTPM010000559 DASTPM010000000
VERSION     DASTPM010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106148"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2255)
                     /locus_tag="VFR42_09890"
     CDS             complement(<1..2255)
                     /locus_tag="VFR42_09890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369511.1"
                     /translation="MAYSKSKYVEAAQKLLNQGKVPQAIAEYQQILKYEPKDQVTLMT
                     IGELYIRQGETFQAIDYFERLAQIFVGDGFLTKAIAVYKRIAKLAPEEIRPLEKLADL
                     YVQQGVMSEARPLFLQLAEIHLKNNKQPEAVALLKKLLLAEPDNLRIQIRLADLYTAM
                     GQTADAIEAYVGAAQRALARGDTAECEKLADRALKLDPKNMAAVIVKARIHSSKGDTA
                     EAAKALEQVADLDKGGEQTELLLDMYLKNSDWKNASALAMRVFDADPKNFGTAQKVTD
                     ALLQADEGEQAMQVLERIRIPMVDSGEQEAVGHLLNELSARLPGRIEPLEWLVEFYGR
                     TNDAFRLTDSLAHLGDALAAAKEYERAKEVYEQLVARQPDSDAPKRKLNAVLRKLGHD
                     VEEEAPVAAATEEPEETLSVDLIKKAAPKLPSATETPEVKAKPAGDSGDSHLDEETQK
                     FIAQSLTDVDLFASYGLTQKAIGLLEAILRRAPRHAPTLEKLLDFVLGAGDDRRTAEL
                     AAQLEQIHTERGDTRSAERFEELRRRFQRAAGLSDEDLAPPQPAAPEPPQAAPEAEAD
                     AEIEEIHVPEETAAAREEVHEVDLSDEWASMLSDTRPADPTGQAPVAEDESAPEFEIP
                     VEAGVPENTNPFAENSQDISVAEELPEISIRKEAHRSASIFEHDDSAADSGASTFRTE
                     PPLDAKPPREKPPVEEKTKVKPKAPQPEPEFEVDQEYELVLDAEPLVPAHEQKPPAPP
                     APRRPEAVQQNA"
BASE COUNT          423 a          767 c          832 g          572 t
ORIGIN      
        1 gcattctgct gcacggcttc gggccggcga ggcgcgggcg gagccggggg cttttgttcg
       61 tgtgcgggaa cgagcggctc ggcgtcgagc accaactcgt actcctgatc tacttcgaac
      121 tcgggctctg gctgcggcgc cttgggtttt accttggttt tttcctcgac cggaggcttt
      181 tccctggggg gcttggcgtc caacggtggt tccgttcgga acgttgacgc tccggaatcc
      241 gccgccgaat catcgtgctc gaaaattgag gcagagcggt gcgcttcctt gcgaatggaa
      301 atctcgggca gctcttccgc gacagaaata tcctgggaat tctccgcgaa gggattggtg
      361 ttctcgggca ctcccgcttc caccgggatc tcaaattcag gtgcggattc gtcttcagcg
      421 acgggtgcct ggccggtcgg gtccgccggg cgcgtgtcgc tcagcatcga ggcccactca
      481 tcggatagat cgacttcgtg cacctcttct ctggccgcgg cagtctcctc cggaacgtga
      541 atctcctcta tttcggcgtc ggcctccgct tcgggcgcgg cttgcggcgg ctcaggcgcg
      601 gctggttgcg gcggagccaa atcttcatca ctgagacctg ccgcgcgttg gaagcggcgc
      661 cgcaattcct cgaagcgctc ggcgctgcgc gtatcgccgc gctcggtgtg gatttgctcg
      721 agttgcgcgg caagttccgc ggtacgacgg tcgtcacccg ccccgagcac aaaatcgagc
      781 agcttttcca gggtgggcgc gtgccgaggg gcgcggcgca gaatcgcctc gagcaagcca
      841 atggcttttt gcgtcagccc ataactggcg aagagatcga catcggtcag cgactgcgcg
      901 ataaacttct gcgtctcttc gtccagatgg ctgtcaccgg aatcgcccgc gggttttgcc
      961 ttcacctccg gcgtttccgt cgcactgggc aatttcggag cagccttctt gatcagatcg
     1021 accgacaagg tctcttccgg ctcttcggtg gcggcggcta ccggcgcctc ttcctcgacg
     1081 tcatggccca gtttccgaag cacggcattg agctttcgtt tgggcgcatc actgtctggc
     1141 tgccgggcaa cgagctgttc gtatacttct ttggcccgct catattcctt cgctgcggcg
     1201 agagcgtccc cgaggtgagc gagcgagtcc gtcaggcgga aggcatcgtt ggtgcggccg
     1261 taaaattcca ccagccattc cagcggttcg atgcgccccg gaaggcgcgc gctcagctcg
     1321 ttcagcaggt ggcctacggc ctcttgttca ccggagtcca ccatggggat tcgaatacgc
     1381 tccaggacct gcatggcctg ttcgccttcg tccgcttgca acagggcgtc ggtgactttc
     1441 tgcgcggtgc cgaaattttt ggggtcggcg tcgaagacgc gcatggccag agcggaggcg
     1501 ttcttccaat cagaattctt cagatacata tcgagcagca actcggtttg ctcgccgccc
     1561 ttatcgagat cggccacctg ctcgagggcc ttggccgcct ccgcggtgtc gcccttcgag
     1621 gagtggatgc gcgccttgac gatgaccgct gccatgttct tgggatcgag cttgagcgcg
     1681 cgatcggcca gcttctcgca ttccgcggtg tcgccgcgcg ccagggctcg ctgcgcagcg
     1741 cccacgtagg cttcgatggc gtcagcggtc tgtcccatcg cggtgtagag gtccgccagg
     1801 cgaatctgga tgcgcaggtt gtccggctcg gccaggagca atttcttcag cagagccacc
     1861 gcttccggtt gtttgttatt ttttagatga atttccgcga gctgcaggaa gagcggccgg
     1921 gcctcgctca tgaccccttg ctgcacatag aggtcggcga gtttctcgag cggccggatc
     1981 tcttcgggag cgagcttggc gatgcgcttg taaacggcga tggccttggt gaggaagccg
     2041 tcgcctacaa agatctgggc caggcgctca aaatagtcga tggcttgaaa cgtttcgccc
     2101 tgacggatgt agagctcccc gatggtcatc agggtgactt gatccttggg ctcgtacttc
     2161 aggatttgtt ggtattcggc gatagcctga gggactttgc cctggttcag gagcttctgc
     2221 gcggcttcga cgtatttgct tttgctgtag gccattcgct aactcagaca cagcgccgca
     2281 tgcgcctgct ggagaggggt cttcctccgc gcccgaaggt aaaaaaggca ggcagaatcg
     2341 gtgcttgcta acgctagcag gcggagaaaa gcgaagtcaa cgaacgcaga atttcgtgtg
     2401 agcaggcagt tatccccgtt ggacttagga acttcgcggc gaaaatcagg cgaagcgcct
     2461 ggagacgcag cagtggccga aatggcgtgg cggctggggc gcgtagcccg gagcgtcgac
     2521 ggaaatcaag atggtggaat ggtgtcgggg agtagcgcaa tctgcgtcgc gcctaccgac
     2581 cagcagcccg cgtc
//
LOCUS       DASTPM010000560         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_913549, whole genome shotgun sequence.
ACCESSION   DASTPM010000560 DASTPM010000000
VERSION     DASTPM010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913549"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..664
                     /locus_tag="VFR42_09895"
     CDS             155..664
                     /locus_tag="VFR42_09895"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU0369512.1"
                     /translation="MAKSTVNEKERMVITRVFDAPRALVWEAWTNPKYVMQWWGPKGF
                     TAPFCEMDFRVGGKFLCCMKSPDGQEFWNGGEYHEIVPHEKIVYSLYFADSKGNKVEP
                     AQYGIEHEAIDDARDVVIFEDFGNGQTKLTMIGNETMEDAKNSGQLEGWNETFEKVAA
                     VIAGLAKAK"
     gene            914..>2085
                     /locus_tag="VFR42_09900"
     CDS             914..>2085
                     /locus_tag="VFR42_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369513.1"
                     /translation="MSFSFFRDLRLAFHSLMHSKSLAAIVILTLALGIGANSAIFTLV
                     RGVLLKPLVNRDENRLVYIRQSAPSGGQDNTTFSVPEIKDIEGGVRSMSEFGDFSTMD
                     FTMVGLGEPRVVHGGVVSGNYFDVMGLRPVLGRLVGPQDAGADAPGVIVLTYRFWSTV
                     YKKDPNVIGKSVRLSSLGDRSATVIGVLEPSVPYPAETEIIGNLVISPHHLSATMVTG
                     RIHRMTELFGKLAPGATLDQARNELRTAYAGMVKDHPDAYPDNFGYRIDARMLRDEIT
                     SGARTVLLVLLAASGLVFIIACSNVANLILARTVRREGELAIRAAMGASTWALRRMLL
                     AESLLLCCAGAAIGVIIAQPLVTILARYASRFSVRALDLTVDFSMLWVGVALAIVAA"
BASE COUNT          441 a          549 c          639 g          456 t
ORIGIN      
        1 aagacgaagc gcggaagaga gagcgtgtgc acgctcaggc cagaggcgat caagaccgtc
       61 agcgactggg ccactaacta tcggcgattc tgggaagaga gtttcgacaa gttggatttg
      121 gttgtgaatc aaatgaagaa agcggaggag agacatggca aaaagcacgg ttaacgagaa
      181 agagcggatg gttatcacca gggtttttga tgccccacgc gcattggttt gggaggcgtg
      241 gacaaacccg aaatatgtga tgcagtggtg ggggccgaag ggttttactg cgcctttttg
      301 cgagatggat tttcgcgtag gaggaaaatt tctttgctgc atgaaatcgc cggatgggca
      361 ggagttctgg aatggcgggg aataccacga gattgttccg cacgagaaga tcgtttactc
      421 gctgtacttt gccgactcga agggaaacaa ggttgagcct gcgcagtatg gaatcgaaca
      481 tgaggccata gacgatgcgc gcgacgtggt tatttttgag gattttggga acggccagac
      541 gaaactcacc atgattggca atgaaaccat ggaagacgca aagaacagcg ggcaactcga
      601 gggctggaat gagaccttcg aaaaagttgc cgcagttatt gcggggctag cgaaggcgaa
      661 ataagaagtt gaaattttga tgatcgtccc gcacgagata cagctacggc cggcggcgga
      721 ggcttagtta gaacgggctt ctcgtcgctt ggccgaatag tcgaatttcc ggctaagtga
      781 tccttggctc aactgttgcc ttcaggccac tgcgctcagc gaccaatgtt taccgaactc
      841 aagatcctat ataaagtggc ggccctgata cggcgtcgct taacgagcgt cgcttaacga
      901 acaaggagaa aaaatgagtt tctcattttt ccgcgattta agacttgcgt tccactcgtt
      961 gatgcacagc aaaagcctcg cggccattgt gatcctcacg ctggcgctgg gaatcggggc
     1021 caactcggcc atcttcacgc tggtccgggg cgtgctgctg aagccgctgg tcaaccgtga
     1081 cgagaaccgg ctcgtgtata tccgccagag cgcccccagc ggcggacaag acaacacgac
     1141 gttttccgtg cccgaaatca aggatatcga gggcggcgtg cgctccatga gcgagttcgg
     1201 cgatttctcg acgatggact ttacgatggt cgggctcggc gaacctcgcg tggttcacgg
     1261 cggcgtggtc agcgggaact actttgatgt gatgggtctg cgtcctgttc taggccgtct
     1321 ggtcggacct caagacgcgg gcgctgacgc ccccggcgtc atcgtgctga cgtatcgttt
     1381 ctggtccacc gtttacaaaa aagacccgaa tgtgatcggc aagagcgttc ggctcagcag
     1441 ccttggcgat cgttccgcta cggtcatcgg cgtgctcgaa ccttctgtgc cgtatcccgc
     1501 ggagaccgaa attatcggca acctggtcat tagcccgcat catctttccg ccacgatggt
     1561 caccggccgg atccatcgca tgaccgaact tttcggtaag cttgctcccg gcgccactct
     1621 ggaccaggcg cgcaacgagt tgcgcacggc gtacgctgga atggtgaagg accatcccga
     1681 tgcgtacccc gataacttcg gctaccgaat tgatgccagg atgctccggg atgagattac
     1741 ttccggtgcg cgcaccgtct tgctcgtgct tctcgcggct tcgggactgg tcttcatcat
     1801 cgcctgctcg aacgtggcta acctgattct ggcgcggacg gttcgccgcg agggcgaact
     1861 cgcgatccgc gccgcgatgg gcgccagcac ctgggcgttg cgtcgtatgt tgttagccga
     1921 aagccttctg ctttgctgcg ccggcgcggc catcggcgta atcatcgcgc agccattggt
     1981 caccatcctg gctcgatacg cctcgcgctt ctccgttcgc gcgctggatt tgacggtgga
     2041 tttcagtatg ctctgggtgg gagtggcgct ggccatcgtc gccgc
//
LOCUS       DASTPM010000561         4638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_279670, whole genome shotgun sequence.
ACCESSION   DASTPM010000561 DASTPM010000000
VERSION     DASTPM010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279670"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFR42_09905"
     CDS             <1..661
                     /locus_tag="VFR42_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU0369514.1"
                     /translation="DPWLLTAEKYPVGTVVTGKVRNIVEFGAFVELEDGFDGLVHVSD
                     VSWTERIKNPHEVFKKGEPVTAKVLRIDPDGRRVSLGMKQVNDIWGDWFKQHKVGQIV
                     KGKVSRIAMFGAFVELGENIEGLCHNTEIMDRRRGDDGSGMNRTTSGPLKSAGPLEPG
                     KEYDFKIIKISPESRRIGLSYKAAVKQVERQEIAQYRSTSRASSTATIGDALKQKLSS
                     R"
     gene            846..1199
                     /locus_tag="VFR42_09910"
     CDS             846..1199
                     /locus_tag="VFR42_09910"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU0369515.1"
                     /translation="MTKQPTLTKADLIEEVLKVTELPRKESEIIVETIFDSIIEAIQK
                     GEKIEIRGFGSFRTRERRGRVGRNPKTGEKVEVPAKRIPFFKPSKELKDFVNSDEAAA
                     MTAAAEAAAGNSSNS"
     gene            1273..1758
                     /locus_tag="VFR42_09915"
     CDS             1273..1758
                     /locus_tag="VFR42_09915"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU0369516.1"
                     /translation="MDYLWTPWRYQYIAQVHSGKQPECIFCDAAERKDDDATLVVYRG
                     TSCFVILNRYPYTSGHVMVVPYAHVAELRLCEPSALTEMMHLAQRMESVLRDLYKPDG
                     MNLGMNLGRAAGAGVVGHLHLHALPRWVGDSNFMTVTSETRVHPEDLSITYERLRKAL
                     V"
     gene            1820..2251
                     /locus_tag="VFR42_09920"
     CDS             1820..2251
                     /locus_tag="VFR42_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369517.1"
                     /translation="MAKKRQAAKKSAKKPAKKKAPAAKRAAVKSGAKVKTKAAAKPKP
                     RRGSARGAEPSSGSASSSLDPRRAGRLRFNDEDQSARRRRINDLQGISMRDRADSESV
                     GELLEEGQTLEAEAVAGVEDALDADQGEIRTHEVPEDDVPE"
     gene            2282..3424
                     /locus_tag="VFR42_09925"
     CDS             2282..3424
                     /locus_tag="VFR42_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0369518.1"
                     /translation="MNLELNEEQLLLQKSVRDFAESEVEPRARELDESGHFPRDLFAK
                     AAELGLTGVALPDNEGGAGFDHISYTIVIEEISRCCASTGVILSVQNSLYCDPIHRYG
                     TDEQKRKFLLPFARGEKIGCYALTEPQAGSNAAALQTKAVKRGDKYVVNGTKAWITNG
                     GAADAAIVYVNTDPTKGEKGITALVVEKGRPGFKVGKEEKKLGISATACCELVLTDCE
                     VPESNRIGNEGEGYKVALSTLDGGRIGIASQATGIAQGAFEAALKYSQERLAFGHPIA
                     QFQAIQFMLADMAMEIDAARLLIRKAAWKQDTGARFSQDAAMAKLFASEMATRVAHKA
                     IQIHGGNGYSREYPVERAYRDARITEIYEGTSEIQRLVISSWALRS"
     gene            3455..4591
                     /locus_tag="VFR42_09930"
     CDS             3455..4591
                     /locus_tag="VFR42_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369519.1"
                     /translation="MLELDCGLEFDPSRDEEFFLALPSRPAVCLIEPKESSAEPFFIR
                     TQDLRRRLQRLLGPADPSSKRLNLRDFAKGLRYRLTPTAFEQNLVYYQQARQLFPKRY
                     KQMLRLRPPAVLKISLRNAYPRCYATRRIPVDANGEPSGGFYYGPFASRKSAEAFAEG
                     VLDLFKVRRCQIKIRRDPTFPGCIYSEMKMCLAPCFAGCSKEEYDTEVGRLVSFLGTG
                     GSSLRDELETDRNRASEELDFEKASLLHKRLDKLDDALRGQTELPRRLQNLNAVIFQR
                     GSEEQSIAIYEVRAGRIADPFLLSFTTLAGEPRSAEQLLRRQLEGQRETGPADLSEHL
                     WLLARWFYSNPRVGAIFFKENMWPYRRMMRACARLLAPPAEPPA"
BASE COUNT         1023 a         1320 c         1402 g          893 t
ORIGIN      
        1 cgatccgtgg ctgctgaccg cggaaaaata tccggtcggc acggtggtga ccggcaaggt
       61 ccgcaacatc gtggagttcg gcgcgttcgt cgagctggaa gacggcttcg acggactggt
      121 gcacgtcagc gatgtcagct ggaccgagcg catcaagaat ccacatgaag ttttcaagaa
      181 gggcgaaccg gtaaccgcca aggtgctgcg cattgatccg gatggccgcc gcgtatcgtt
      241 gggcatgaag caggtcaacg acatctgggg cgattggttc aagcagcaca aggtcggcca
      301 aatcgtgaag ggcaaagtct cgcgcatcgc catgttcggc gcgttcgtgg aactgggcga
      361 aaacatcgag gggctctgcc acaacacgga aatcatggac cgccgacgtg gcgacgatgg
      421 cagcgggatg aaccgcacca ctagcggccc gctgaagagc gcaggtccgc tcgagccggg
      481 caaggaatac gacttcaaga tcatcaagat cagcccggag tcgcggcgca tcggcttgag
      541 ctacaaagcg gcggtgaaac aggtggagcg gcaggagatc gcgcagtatc gctcgacctc
      601 gcgggcgtct tcgacggcaa ccatcggcga cgcgctgaag caaaaactct cttcgcggta
      661 gccgcaattc cattttttga ccgtggggcc agtttttaat tcactggccc ctaatttttt
      721 gttttgccca caagagcatt tggtgatagg aagtgagcca gcgggcgtac aaatcttccc
      781 gtaagccctt ccctttcatg ggtctgcgac gtatcatcta ggaactttag cgaggcgtgg
      841 ccacgatgac caagcaaccg acgttgacga aggcggacct gatcgaagaa gtgttgaagg
      901 tcacggagct tccgcgcaag gaatccgaga tcatcgtgga aaccattttc gacagcatca
      961 ttgaggctat tcaaaaaggc gaaaagatcg agattcgcgg attcggcagc tttcgcacac
     1021 gcgagcggcg tggccgcgta ggccgcaatc ccaagaccgg cgaaaaagtg gaagttcctg
     1081 ccaagcgcat tccttttttc aagccgagca aagaactgaa ggatttcgta aacagcgacg
     1141 aagccgcggc catgaccgca gccgcggaag ccgctgcggg caactcttcg aactcctgat
     1201 ggccgccttg gtttcgccgt ttgccggcgt tcatcccgat tttttgaact ttcgcgcgag
     1261 gtttcgctcg caatggatta tctctggacg ccgtggcgct atcagtacat tgcgcaggta
     1321 cattccggaa agcagccgga gtgtattttc tgcgacgccg cggaacgcaa agacgacgac
     1381 gccaccctgg tggtgtaccg gggaacttcg tgcttcgtga ttttgaaccg gtatccctac
     1441 acatcgggtc acgtgatggt ggtgccgtac gcgcatgtag cggaattgcg attgtgcgag
     1501 ccgtcggcgc tgacggaaat gatgcatctg gcgcagcgca tggaatcggt gctgcgcgat
     1561 ttgtacaaac cggacggcat gaacctgggc atgaatttgg ggcgcgccgc gggcgcgggc
     1621 gtcgtgggac atttgcactt gcacgcatta ccgcgctggg tgggtgattc gaactttatg
     1681 accgtgacca gcgaaacgcg cgttcacccg gaagatttgt cgatcacgta cgagcggctg
     1741 cgcaaagcct tggtctgatt ttgttttccg attgtttttc gtaacatgga agactcccac
     1801 ccgcagagag aggttcgtca tggcaaagaa acgccaagcc gccaagaaat cagcgaaaaa
     1861 gccggccaag aagaaagcgc ctgccgcgaa aagagcggcc gtcaagtccg gcgcgaaagt
     1921 taaaacgaaa gccgccgcca agccgaagcc gcggcgtggt tcagcgcgcg gggccgagcc
     1981 gtcttccggg tccgcttcga gttcgctcga tccacgtcgc gccggccgcc tgcgcttcaa
     2041 cgacgaagat caaagtgcgc ggcggcgccg catcaatgac ttgcaaggca tcagcatgcg
     2101 cgatcgagcc gattcggaga gtgtgggcga attgctggaa gagggacaaa cgctggaagc
     2161 cgaggcggtg gcgggagtcg aagacgccct ggacgccgac cagggcgaaa ttcgcacgca
     2221 tgaagtgcct gaggacgatg ttccggaata atatctcgcc catgacaggt agcgcgcaca
     2281 agtgaatctg gaactgaacg aagagcaact gctgctgcag aaatcggtgc gcgattttgc
     2341 cgaatccgaa gtggaaccgc gcgcgcggga attggatgaa agcgggcatt tcccgcggga
     2401 cttgtttgcc aaggccgctg aactggggct aaccggcgtg gcgctgccgg ataacgaggg
     2461 cggcgctgga ttcgatcaca tctcttacac catcgtgatc gaagaaattt cgagatgctg
     2521 cgcttccacc ggcgtaatcc tttcggtgca aaattcgctg tattgcgatc cgattcaccg
     2581 ctacggcacc gacgagcaga agaggaaatt tctgctgccg ttcgcgaggg gcgaaaaaat
     2641 cggctgctac gcgcttacgg aaccgcaggc cggctcgaat gccgccgcgc tgcaaaccaa
     2701 agccgtaaag cgcggcgaca agtatgtagt gaatggcacg aaagcgtgga tcaccaacgg
     2761 cggcgcagcg gatgccgcga tcgtgtatgt aaacactgat ccgaccaaag gcgaaaaagg
     2821 aatcaccgca ctggtcgtgg aaaaaggcag gccgggcttc aaggtcggca aggaagaaaa
     2881 gaaactcggc atcagcgcga ccgcgtgctg cgagctggtc ttgaccgatt gcgaagtacc
     2941 ggagtccaat cgcatcggca acgaaggcga aggctacaag gtggctttgt cgacgctgga
     3001 cggcgggcgc atcggcatcg cctcgcaggc caccggcatc gcgcaaggcg cgttcgaagc
     3061 ggcgctgaag tattcgcagg agcgcctggc cttcggccat cccatcgcgc agtttcaagc
     3121 catccagttc atgctggccg acatggccat ggaaatcgac gcggcgcgtt tgctgattcg
     3181 caaggccgca tggaaacaag acaccggcgc gcgcttcagc caggatgcgg ccatggccaa
     3241 actcttcgcc agcgaaatgg ccacgcgcgt ggcgcacaag gccatccaga ttcacggcgg
     3301 taacggctac agtcgtgaat atccagttga gcgcgcctat cgtgacgcgc gcatcacgga
     3361 aatttacgaa ggtacaagcg agattcaacg cctggtgatt tcttcctggg cgctgcggtc
     3421 gtgatgtcta actgtaacgt agtattctga ttccgtgctc gaactcgact gcggcctcga
     3481 attcgatccg tctcgcgacg aagaattttt tctggcgctc ccgtctcgac ccgccgtttg
     3541 cctgattgaa cccaaagagt cttccgccga accatttttc atccgtaccc aggatttgcg
     3601 ccggcggctg cagcgtttgc ttggccccgc cgatccatcc agcaagcggc tgaacctgcg
     3661 cgattttgcc aaaggcttgc gttaccggtt gacgccgact gccttcgaac agaacttggt
     3721 ctattaccag caggccaggc agctttttcc caagcgctat aagcaaatgc tgcgtcttcg
     3781 tccgcccgca gttctgaaga tcagcttgcg caacgcttat cctcgttgct acgccacacg
     3841 ccgcattccc gtcgacgcga acggtgaacc gtccggtggc ttttattacg gcccgttcgc
     3901 gtcgcgaaaa tcagccgaag catttgcgga gggcgtgctc gatctattca aggtgcgccg
     3961 ctgccagatc aagattcgcc gcgatcccac gttccccggt tgcatctact ccgagatgaa
     4021 aatgtgcctg gcgccgtgtt ttgccgggtg cagcaaggaa gagtacgaca cggaagtcgg
     4081 acgcctcgtt tcgttcctgg gaacgggagg ctcttcgctg cgcgacgaac ttgaaaccga
     4141 ccgcaaccga gccagcgagg aattggactt cgaaaaggcc tcactgcttc acaaaaggct
     4201 ggacaaactg gatgacgccc tgcgtggtca gactgaactg ccgcgccgct tgcaaaatct
     4261 caacgcagta atttttcagc gcggcagcga agagcaatcc atcgccatat atgaagtccg
     4321 cgccggccgc atcgccgatc cgtttctgct ttccttcacc acgctggcag gtgagccgcg
     4381 ctccgccgag cagttgctcc ggcgacagct tgagggtcag cgcgaaacag gcccggccga
     4441 cctgtcggaa catttgtggc tgctggcgcg ttggttctac agcaatccgc gtgtcggagc
     4501 gattttcttc aaggaaaaca tgtggccgta tcgcaggatg atgcgcgctt gcgcccgcct
     4561 gctggcgccg cctgccgaac caccagctta gggcgcccca aattcggccg gcttcaactc
     4621 gcgcatcgcc tttaaagc
//
LOCUS       DASTPM010000562         3735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_539011, whole genome shotgun sequence.
ACCESSION   DASTPM010000562 DASTPM010000000
VERSION     DASTPM010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539011"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFR42_09935"
     CDS             complement(<1..278)
                     /locus_tag="VFR42_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0369520.1"
                     /translation="MTQSDSHSALIRPAATGGFALGLGLTNECNLACSFCYRDPARTD
                     RLGLEQVRSVMESLPVRSVNLGTGENGMHPQFKEILAYLRSLAVKLTIT"
     gene            479..1579
                     /locus_tag="VFR42_09940"
     CDS             479..1579
                     /locus_tag="VFR42_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEU0369521.1"
                     /translation="MKRREFLQSGSIASASLAFAKPKRFLSLASASDGWRTFQVTTRV
                     EVLKPSGTTRIWVPAALLSETPFQKTLSNTFNCEGGTAKLMENKSDSLGIMAAEFPAG
                     VRPALTVTSRVATKDRAVDLTAPGAAPSESHAELEHFLRPTKLLPTDGIVKETASEIT
                     QGAKTDVDKARAIYEWIVENTFRNPKTRGCGVGDIRFMLESKDLGGKCADLNALFVGL
                     ARAAGLPARDVYGIRVAKSELGYKSLGASSENVTKSQHCRAEVYLGARGWVPVDPADV
                     RKVVLEEPPGNRPLDDDMVKKARARLFGSWEMNWMAYNFAHDVALPGSNGAPVGFLMY
                     PQAETSEGRIDSLDADNFKYEITSREITSANG"
     gene            complement(1642..3474)
                     /locus_tag="VFR42_09945"
     CDS             complement(1642..3474)
                     /locus_tag="VFR42_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HEU0369522.1"
                     /translation="MKVPGLLRAMLSAWLSLFAVVAASVQEAGKTDSSFVARFSDITD
                     RAGVKFRYLSSHTTRKYLPETMGAGVALFDYDNDGRLDIFLVNGAPIEDPTAKGSIPR
                     KAGPEYWNRLYHQKPDGTFEDVTEKAGLQGAGYGMGVAVGDYDNDGFEDLYVTAYGGN
                     RLYHNNGDGTFTDVTQSAGVGGSGWSTSAAWVDLDNDGFLDLVVLRYVEWDFDDIWCG
                     EHREGFRAYCHPDYFKAAVPLVYHNNGKGTFTEVAQKVGLAKPAKGLGIAIADYDRDG
                     HIDLFFANDSMVEYLYRNKGNGTFEEVGLLSGVAADIDGHTYAGMGVDFSDYDNDGWP
                     DIVVTNLANQRYALYHNNGGGTFDYCSPAAAISRITMTHSGWGVRFFDFDNDGRKDLL
                     VAQGHDLDTIEKTSPNLRYRESLLLARNTGKEFQDVTPAAGPALQQEWVWRGLAIGDL
                     DNDGRLDAVATANDGPVRVFHNETTTNNHWILLQLAGHTSNRDAIGASVKIVTADGAQ
                     YATVSTASSYLSSGDKRVHFGLGKASVVQTIEIRWPSGIAQTLKDVAADKIVQIDEPA
                     NNLPPLPEKQSSLPLTRLYPIGTNKPHHSSAWVREIPPKWVPLR"
     gene            complement(3471..>3735)
                     /locus_tag="VFR42_09950"
     CDS             complement(3471..>3735)
                     /locus_tag="VFR42_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369523.1"
                     /translation="VSYLTQAVQIAPRESRAREKLGSAYLNLNDLPAAQEQLERAVGL
                     DPRNPSSHYLLGSVYRKQGHAEKAKAEFDRFQTLKEVTGTLRP"
BASE COUNT          795 a         1086 c         1077 g          777 t
ORIGIN      
        1 gtgatagtga gctttaccgc cagagagcgg aggtaggcca aaatctcctt gaactgcggg
       61 tgcatcccgt tctcaccggt ccccagatta acggagcgga ccggaaggct ttccatcacc
      121 gagcggactt gctcaaggcc aagccggtcg gtgcgcgccg gatcgcgata gcagaaagaa
      181 caggccaggt tgcactcgtt ggtcaggccg agtccaaggg caaatccccc ggtagcagca
      241 ggccggatca acgcagagtg tgagtcggac tgagtcatcg tcccttctgt tcaccgatga
      301 cgcacccgca tcttccagtc gcgtcatcca ttgcagaaca ctagcaccat cattgcatat
      361 ccgaaacact ctgacgccga ctgaacccag attcctgccc gaggcattgt atccggacgc
      421 cgccgcgtgc atcctatcgg tcagcaggca agaaaaccgg cctggctgga ggaggggcat
      481 gaagcgtcgc gaatttctac aatcgggaag cattgcgtca gcaagcctcg cgttcgcgaa
      541 gccgaagcgt ttcctttcac tggccagcgc gtctgacggt tggcgcactt ttcaggtaac
      601 aacgcgcgtc gaagttctga agccttccgg gacgacacgc atatgggtgc cggcggccct
      661 ccttagtgaa actccttttc aaaagaccct cagcaatacc ttcaactgcg agggaggcac
      721 cgcgaaattg atggagaata agagcgactc gctcggaatc atggcggcgg aattccccgc
      781 cggggtacgg ccagcgctga cggtaactag ccgggtcgcc acgaaagatc gcgccgtcga
      841 cctcaccgcg ccgggagcgg cgccaagcga gagccacgcg gaattggagc atttcttgcg
      901 gccgaccaag cttctgccga cggacggcat cgtgaaagag accgcaagcg aaatcacgca
      961 aggtgctaag accgatgtcg acaaggcgcg tgcgatctac gagtggatcg tggaaaacac
     1021 cttccgcaac cccaaaacgc gcggctgtgg cgtcggcgac attcgcttca tgctggagtc
     1081 caaagatctg ggcggcaagt gtgcggacct aaacgcgctg ttcgttggat tggcgcgtgc
     1141 cgcgggcctt cccgcgcggg acgtttatgg catccgcgta gccaaatctg aattgggata
     1201 caagagcctc ggtgcttcgt cagagaacgt gacgaaatcg cagcattgcc gcgcagaggt
     1261 ttatcttggc gcgcgcggat gggtccccgt tgatccggcc gacgtgcgca aggtcgtgct
     1321 cgaggagccg cccggaaatc gtccgctgga tgacgacatg gtgaagaagg cccgcgcacg
     1381 cttattcggt tcgtgggaaa tgaactggat ggcgtacaac ttcgctcacg atgtcgcact
     1441 gcccgggtcg aacggcgcgc cggtaggttt tctcatgtac ccgcaagcgg aaacaagcga
     1501 aggccgaatc gacagcctgg atgcggacaa tttcaaatat gagatcacct cgagggagat
     1561 cacgtctgca aacggctgac gaaaacggac gtagaaaggc cgttcttggc aacttaccgc
     1621 ccgagaatgc agctaccgaa gtcagcgcag gggaacccat ttaggaggta tttctcggac
     1681 ccacgccgaa gagtggtgtg gtttgttcgt tcctatcgga tacaggcgcg taagtgggag
     1741 gctggattgt ttttccggga gcggaggcag gttgttcgct ggctcgtcaa tttgcacaat
     1801 cttgtccgcc gctacatctt tcagcgtttg tgcgatgccg ctgggccagc gaatttcgat
     1861 cgtttgaacc acgcttgctt tccccagccc aaagtgaacg cgcttatctc ccgaagaaag
     1921 atagctgctc gccgtgctga ccgtggcgta ttgcgcgccg tctgcagtga caatttttac
     1981 tgaagcgcca atcgcgtcgc gattgcttgt gtgtccggcc agctgcaaca aaatccaatg
     2041 attgttggtg gtggtttcat tgtgaaaaac gcggaccggg ccgtcattcg cggttgcgac
     2101 agcgtctagc cgcccgtcgt tgtccaggtc gccgatcgcc agtccccgcc acacccattc
     2161 ttgttgcaaa gcgggcccag cagccggcgt gacatcctga aattccttac cggtgttgcg
     2221 ggcaaggagc agcgattcgc gataccgaag attgggagac gttttttcga tcgtgtccag
     2281 gtcatgccct tgcgcaacca gcagatcctt gcggccatcg ttgtcgaagt cgaaaaagcg
     2341 cacgccccat cctgaatggg tcatggtaat tcggctgata gcggccgccg ggctgcaata
     2401 gtcaaacgtt ccgccgccgt tgttgtgata cagcgcgtag cgctggttcg ccagattagt
     2461 cacgacgatg tccggccagc cgtcattgtc gtagtcggaa aaatctacac ccatcccagc
     2521 ataggtgtgg ccgtcaatat ccgccgccac gccagaaagc aatccaactt cttcaaacgt
     2581 gccgtttccc ttattgcggt agagatactc caccatcgaa tcgttggcaa aaaaaagatc
     2641 aatgtggccg tcgcggtcgt aatccgcaat cgctatgcct agtcctttgg ccggtttggc
     2701 caaaccaacc ttctgcgcca cttcggtaaa agttcccttc ccattgttgt gatacaccag
     2761 cgggacggcg gccttgaaat agtcgggatg gcagtaagcg cgaaaaccct cgcggtgttc
     2821 gccgcaccag atatcgtcga agtcccactc gacgtaacgc agcaccacca ggtccagaaa
     2881 gccgtcattg tcgaggtcaa cccaggccgc gctggtggac cacccggagc cgccgacgcc
     2941 cgcgctttgg gttacatcgg tgaatgttcc gtcaccgttg ttgtgataga ggcgattgcc
     3001 gccgtatgcc gtgacgtata aatcctcgaa accgtcgttg tcgtaatctc cgaccgcgac
     3061 tcccatgcca tagccggcgc cctggagccc ggctttttcg gtaacgtctt caaacgtgcc
     3121 gtcgggtttc tggtgataca gacgattcca atattccggt ccggctttgc gcggaatgga
     3181 tccttttgcc gtagggtctt cgatgggagc gccgttaacc aggaaaatat ccaagcgccc
     3241 gtcgttgtcg tagtcaaaca acgcgacgcc agcacccatg gtttccggca gatattttct
     3301 ggtggtgtgc gaggacaagt agcggaattt cacgccagcg cggtccgtga tgtcggaaaa
     3361 tctggcgacg aacgacgaat cggtcttccc cgcttcctgc acggaagccg caaccacggc
     3421 aaagagactc aaccacgccg acagcatcgc gcgaagcaga ccggggactt tcacggccgc
     3481 aaagttcccg ttacttcttt cagtgtctgg aaccgatcga actcggcctt ggctttttcc
     3541 gcgtggccct gctttcgata aacgctgccc aacagatagt gcgaactggg atttcgcgga
     3601 tccaggccaa ccgccctttc taattgttcc tgtgcggcgg gaagatcgtt taggttcagg
     3661 taggcgcttc ccagcttttc gcgtgcgcgg ctctcgcgtg gtgcaatctg caccgcctgc
     3721 gtcaaatacg aaacc
//
LOCUS       DASTPM010000563         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_423656, whole genome shotgun sequence.
ACCESSION   DASTPM010000563 DASTPM010000000
VERSION     DASTPM010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423656"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFR42_09955"
     CDS             <1..761
                     /locus_tag="VFR42_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369524.1"
                     /translation="LAAAGFFILGFGHPSFWFLGLATEAIVLPSLAFNRRFQNVVDAE
                     DRQLASGDSKAKRASLVQLLPPDDKRRLAALERKCDKVIEVYRNAQAEDFLIDTNRDA
                     LENLKWIYLKLMIARYHLLTAGTDDTPAALAKKIDTLQGELKNSQDSAALRQSRSATL
                     DILKRRLANVQRREESLEEVESDLTRVESQVDLILDNAAMQGKPQTISTDLELASDLV
                     SGGMFGDAESAVADLDRDYGQPRAVSKAATPEHS"
     gene            761..2464
                     /locus_tag="VFR42_09960"
     CDS             761..2464
                     /locus_tag="VFR42_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0369525.1"
                     /translation="MSTLPPQAVPGNTTREAARALPAWAQSLAESYESNAASQFVIYG
                     NISDRMVLPGSPAPRLSSLADFLLTVMLSRFDVVLSYDIGNGIRVEKGAETFSKWPHF
                     QESQKDWKAPRPAIETLTRYFRYCANVTRLSQPSVQVGCIIKSADLLVPALQGGLDYD
                     LNALASLIRDWSSESLLAGHALATFLLTENLNDLHSLLVNNPRSARLRIDLPSSEELL
                     AGFRVLAPSFPVSLAAVAANLSAVAEQLAGTTLGAVESAVKRKEHKGEALAAGDLVKL
                     KKELVEKDTSGLIEFIETSRTLNDVYGLEGIKSWLRQDIELWKKNDVAALPKGHLLCG
                     PVGTGKTFLVECLAGEASVPIVKMKNFRDKWVGSSEGNLEKIFRLIHALGRCYVFIDE
                     ADQALGRRDAAGGDSGVGGRIYSMLAEEMGSSANRGRVIWVLASSRPDLIEVDLKRPG
                     RVDVKIPLFPTTTAREGFDLLRMLFAKRGLTVPEDAFASLEPSIPRLLTPGAAEALVM
                     RVYRQVRTSEATLDAVLRDCLVEYQNPIAPEVMEFQIRTAVNEASALEFVPQTFRRKT
                     P"
     gene            2698..>3443
                     /locus_tag="VFR42_09965"
     CDS             2698..>3443
                     /locus_tag="VFR42_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0369526.1"
                     /translation="MQPSVLAWAMAATVGLVVAEVFGGLLGHSIALLNDAVHNLSDVP
                     ALGISWLAMRWAQRPADDEKTYGYRRAGELAAFTNAVLLVLLAVWLGWESIERLRAPQ
                     NVVESWMIWTSIAALSVNGGITLALVRGRNDLNLRSILVHNFGDALSNVAIIVGAIVI
                     GYTGWHRIDPILGLAIGLLVLWSSIGILRASAHVLLEGRPKEMRIEEVARAILRVDGV
                     MEVHDVHIWSLGGGEIALSCHARVPDMHMD"
BASE COUNT          738 a          906 c         1126 g          673 t
ORIGIN      
        1 ccctggcggc ggccggattc tttattttag ggttcgggca tccgagtttt tggtttctcg
       61 gactcgctac agaagccatc gtcctgcctt ctctagcctt caatcggcgg tttcaaaatg
      121 tggtcgacgc ggaagaccgg caattggcca gcggagattc aaaggccaag cgcgcttcgc
      181 tggtgcaact cctgccccct gacgacaagc gtcggctggc ggcgttggag cgcaaatgcg
      241 acaaagtcat agaggtttac cgcaacgcgc aagcggagga ttttctgatc gacaccaatc
      301 gagacgcgct ggaaaacctg aaatggatct atctgaaact gatgattgcc cgctatcacc
      361 tgcttacggc gggcacggat gatacgcccg cagcgctggc aaaaaagatc gataccctgc
      421 aaggggagct gaaaaacagc caggactcgg cggcgcttcg gcaatcgcgg tcggcgacgc
      481 tggatatcct gaaacggcgg ctggcgaacg tgcaacgccg ggaagagtca ttggaggaag
      541 tggaaagcga cctgacgcgc gtggaatcac aagtcgacct gattctggac aatgccgcga
      601 tgcaaggaaa gccgcagacg atttccacgg accttgaact ggccagcgat ctggtgagcg
      661 gcggaatgtt tggcgacgcg gaatcggccg tagccgatct ggaccgggac tacggccaac
      721 cacgggccgt gtcgaaggcc gcgactccgg agcactcctg atgagtacac tgccgccgca
      781 ggctgtgcca gggaacacga cgcgagaggc ggcgagagcg ctgccggcgt gggcgcagag
      841 tcttgccgaa tcctacgaaa gcaacgcagc gagccagttc gtaatttacg gaaacatcag
      901 cgaccggatg gtgctgccgg gatcgccggc tccgcgtctg agctcgctgg cagactttct
      961 acttacggtg atgctgtcac gcttcgatgt agtgctgagc tacgacattg gaaacggaat
     1021 ccgtgtagag aaaggcgctg agacattttc aaagtggccg catttccagg agagtcaaaa
     1081 ggattggaaa gcgccacgac cggcgatcga aacgctgacg cgttattttc gttattgcgc
     1141 aaacgtcacc agactcagcc agccgtcagt tcaagtgggc tgcatcatca aaagcgccga
     1201 tttgctggtg ccggcgttgc aaggtggact ggactatgac ttgaatgcac tggcatcgct
     1261 gatacgcgac tggtccagtg agagcctgct ggccgggcat gcgctggcaa cgtttctgct
     1321 cacggaaaat ctgaacgatc tgcattcgct gctggtgaat aatccccgct cggcgcggct
     1381 gaggattgac ctgccatcgt ctgaggaact tctggctggc tttcgcgtcc tggcgccttc
     1441 gtttccggtt tcccttgccg ctgtagctgc caatctctct gcggtggcgg agcaactggc
     1501 gggcacgacg ctgggggcgg tagagagcgc ggtaaagagg aaggagcaca agggtgaggc
     1561 actagccgca ggcgacctgg tgaaattgaa gaaagagctg gttgaaaagg atacgagcgg
     1621 gctgatcgaa tttatcgaga cgtcgcgaac gctcaatgac gtatatggac tagaagggat
     1681 caagtcgtgg ctgcggcagg acattgaact ctggaaaaag aatgacgttg cggctctccc
     1741 caagggccat ctgctgtgcg gcccggttgg cacaggcaaa acctttcttg tcgagtgcct
     1801 ggcgggcgaa gccagcgtgc cgattgtgaa gatgaagaat ttccgcgaca agtgggtggg
     1861 aagcagcgaa ggcaacctcg aaaaaatatt tcgactgatc cacgcgctgg gccgttgcta
     1921 tgtgtttatc gacgaagccg atcaggcgct tgggcggcga gacgcggcgg gcggcgactc
     1981 cggtgtcggt ggacgaatct attccatgct ggcggaggag atgggtagca gcgccaaccg
     2041 cggcagagta atctgggtgc tggcgtcgag ccggccggac ctgatcgaag tagacttgaa
     2101 gcgccctggc cgtgtggacg taaagattcc gctgtttccg acgacgacag cccgcgaagg
     2161 atttgatcta ctgcgcatgc tgtttgcgaa gcgaggcctc accgttccag aagacgcatt
     2221 tgcttcgctt gaaccgagca ttccgcggct gctgacgccc ggagcggcag aggcattggt
     2281 aatgagagtt taccgccagg tacgaacgag cgaagcgacg ctggatgcgg tcttgcgcga
     2341 ttgcctggtc gaatatcaga atcccatcgc gcccgaggtg atggaatttc agattaggac
     2401 agcagtcaac gaagcaagcg ccctggaatt cgtaccccag acttttcgac gaaagacacc
     2461 gtagcggcga tggagcaatg tcgcagtcac gcgagagcgt tggtgattcg aaatgatgct
     2521 agagggcgag taatctagcg gaagatgcat atccacgatc acagccacgg cgaagggcag
     2581 ccgcaccagt gcggagatgc tccgtcgcac ggacactcct tcaaggacca ttcccacgat
     2641 cgtgatcatt ctcatgctca tggattgggc ggccacgcgg gacacgcgca tccgcccatg
     2701 caacccagcg tcctggcttg ggcgatggcg gcgaccgtgg gattggtagt ggcagaagtt
     2761 ttcggtggct tgctgggaca cagcatcgcg ctgctgaatg atgcggtaca caacctttcc
     2821 gacgtgcccg cgctgggaat ttcgtggctg gcgatgcgct gggcgcaacg gccggcggac
     2881 gatgagaaaa cttacggata ccggcgcgcc ggagagctgg cagcgttcac caatgcggtg
     2941 ttgctggtgc tgcttgcggt atggctgggg tgggaatcga tcgaacgtct gcgcgcgccg
     3001 caaaatgtgg tggaatcgtg gatgatctgg acgtccatcg cggcattgtc agtgaatggc
     3061 ggaatcacgc tggcgctggt ccgtgggcgc aacgatttga acttgcgcag cattctggtg
     3121 cacaacttcg gggacgcgct ttccaacgtt gcgatcatcg tgggcgcgat cgtgatcggc
     3181 tacaccggct ggcaccggat tgatccgatc ctcgggcttg cgattggttt gctggtgctg
     3241 tggtcgagca tcggaatttt gcgcgcatcg gcgcacgtac tgctggaggg gcgcccgaaa
     3301 gaaatgcgga tcgaggaagt ggcgcgcgcg atcctgagag ttgacggcgt gatggaagtt
     3361 cacgacgtac acatctggtc gctgggcggc ggtgaaatcg cgctgtcgtg ccacgcgcgc
     3421 gtgcccgaca tgcacatgga caa
//
LOCUS       DASTPM010000564         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_192962, whole genome shotgun sequence.
ACCESSION   DASTPM010000564 DASTPM010000000
VERSION     DASTPM010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192962"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1268
                     /locus_tag="VFR42_09970"
     CDS             222..1268
                     /locus_tag="VFR42_09970"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369527.1"
                     /translation="MRHIEILDDGKNMRIESKAPTRVDLAGGTLDIWPLYLFHPGAVT
                     INAAVTRYASCVIETHARGDEKIRLVSRDTQREENFASFAAMVKAKRYHLPLLAEIAK
                     FFAPEDGFTLTTDSEAPAGAGIGGSSAMAVAICAALDRFTAAGKSKVDWIHISRDAEG
                     IVIKVPTGTQDHYPPAFGGAAAIELPPGGERRVELRLDIRELEKRLVLCYTGKPRQSG
                     INNWEVFKAHIDGKKQVRANLERIAEVAQALRVALENTDWSETGRLMREEWNFRKRNL
                     PTISTNTIDRIIEGARKKGALSGKVCGAGGGGCVVLLIEPDARPTVEQAVAKAGGQLL
                     PMKIDRSGVQVTVS"
     gene            1365..2285
                     /locus_tag="VFR42_09975"
     CDS             1365..2285
                     /locus_tag="VFR42_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369528.1"
                     /translation="MPNALAITVICSSLLLFADADLPRVTSLRSPDATSKPDFSNSEI
                     ALFPPLTSEDPQGQAAASARPHKDKLQDSSKLDLIRYVSGEFAKAAKSLPAGKEGFLQ
                     YADKPLNQELLERAVATHGAAVHAGDNAQITKLEFRDHTIVVDVNGGGRGKKRFLDHL
                     QVSMGGMPTMRSSSDVQQEGGPAGVQPGAGSTLFLEFSKNIPDLTPDELKQLLAPFLD
                     FAKQRSASVQWFDTLPPDIKKAIQDRVPKLGMDREMVVAAIGKPGHKVRERDSEGNEI
                     EDWIYGTPPDKTMFVRFMGDRVTSIRQFPQ"
     gene            complement(2299..>2781)
                     /locus_tag="VFR42_09980"
     CDS             complement(2299..>2781)
                     /locus_tag="VFR42_09980"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU0369529.1"
                     /translation="VQAPKPIPTSFAHENFFSVSAYKFTNAEGVGKFGRYRILPVEGT
                     AYLDTETAKKQSPNFLFDELRGRLAAAPVKLRIVVQVAAEGDVVDDSTSHWPADRPLV
                     EFGTLELSAAVPDNDAEQRHIIFDTIPRVQGIESSGDPLLDPRANLYLASGRRRRASA
                     "
BASE COUNT          600 a          852 c          811 g          518 t
ORIGIN      
        1 acgcgccgtc gcttccgccg ccgaatagtc gctgctttgg gctcttggca cttcgtcgct
       61 cgccgctatt ttctcgcccg gcgacgccgc agggctcctg ccttcgcggg aaccccacgg
      121 aacttgcaac aattccccaa cgtaaatcag tgggcaagcg ttagcagcga agcacgcagc
      181 aacaatttcg cgccctggtg gcttgcaatc ccgcgctatc cttgcgacat atcgaaatcc
      241 tggacgacgg gaaaaatatg cggattgaat cgaaagcacc tacgcgcgtt gacctggccg
      301 gcggaactct ggacatctgg ccgctctatc ttttccatcc cggcgcagta accatcaacg
      361 cggcggtcac gcgctacgcc agctgcgtaa tcgaaacgca tgcgcgtggc gacgagaaaa
      421 tccgtttggt ctctcgcgat actcagcgcg aagaaaattt cgcgtcgttc gccgcgatgg
      481 taaaggcaaa gcgataccac ctgccgctgc tggcagagat cgcgaaattc tttgctccag
      541 aggatggctt caccctcacc accgattccg aagccccggc tggtgcggga atcggaggct
      601 cgagcgccat ggctgtagcg atttgcgcag cgctagaccg gtttaccgcg gcgggtaaaa
      661 gcaaagtcga ctggattcac atcagccgcg acgccgaggg gattgtcatc aaggtgccaa
      721 ccggcacgca ggaccactac ccgccggcat ttgggggagc cgccgccatt gaattgccgc
      781 ccggcggcga acgtcgcgtc gaattgcgcc tggatatccg cgaactcgaa aaacggctgg
      841 tgctttgtta taccggcaag ccgcggcaat ccggaatcaa caactgggaa gtgttcaagg
      901 cgcacatcga tggtaagaag caagtgcgtg ccaatctcga gcgtattgcc gaagttgcgc
      961 aagccttgcg cgtcgccttg gaaaacaccg actggagcga aaccggccgc ctgatgcgcg
     1021 aagagtggaa ttttcgcaaa cgcaacctgc cgaccatttc gactaacacc atcgaccgca
     1081 tcatcgaagg tgcgcgcaag aagggcgctc tatccggcaa ggtttgtggc gcgggtggcg
     1141 gcggctgcgt ggtcctgctg attgaacccg atgcccgtcc taccgtcgaa caagctgtgg
     1201 ccaaggccgg gggccagctc ctgcccatga aaatcgaccg gtctggcgtc caagttaccg
     1261 tctcctgatc ccccttcgaa acccgccgga ggaacccaca ggcatctatt ttttaggctg
     1321 cgagcgtcgt agaatacaga agacgggcca cgccccggag atttatgccg aacgcgctcg
     1381 ccatcacggt catttgctcc agcctgttgc tctttgccga cgcggacctt ccgcgcgtca
     1441 cgagcctgcg cagtcctgac gccacgagca aacccgattt ctccaatagt gagatagcgc
     1501 tctttccgcc ccttacttct gaagacccgc agggccaggc tgctgcatcg gcacggccac
     1561 acaaagacaa gctccaggac agctcgaaac tcgacctgat tcgctacgtt tccggtgaat
     1621 tcgctaaggc tgccaagtcg ctgcccgccg gcaaagaggg ctttttgcaa tatgcggata
     1681 agcccctgaa tcaggaactg ctggaacgcg ccgtagcaac gcacggcgcg gcggtgcatg
     1741 ccggcgataa cgcccaaatc accaagctgg aatttcgcga tcacaccatc gtggtggacg
     1801 tgaatggcgg cggccgcggc aagaagcgtt ttctcgatca cctacaggtg agcatgggcg
     1861 gaatgcccac gatgcggtcc agcagcgacg tgcagcagga aggcggcccg gcgggagttc
     1921 agccgggggc gggcagcacg ctctttctgg agttcagcaa gaatattccg gatttgacgc
     1981 ccgatgagtt gaaacagctt ctggcgccgt tcctggattt tgccaaacag cgttcggctt
     2041 cggtgcagtg gtttgacacg ctgccaccgg acatcaagaa ggccatacag gaccgcgtcc
     2101 cgaaactcgg catggaccgc gaaatggtgg tagcggccat cggcaagccc ggccacaaag
     2161 tccgcgaacg cgattccgag ggaaacgaaa tcgaagactg gatctacggc acgccacccg
     2221 acaagaccat gtttgtgcgg ttcatgggcg accgggtcac cagcatccgc caatttcccc
     2281 agtaatcaag cttaacaatt aagccgaagc tcgccgccgc cgcccgctcg ccaggtagag
     2341 attggcgcgc ggatcgagca gcggatcgcc ggaactttca attccctgaa cccggggaat
     2401 cgtatcgaag atgatgtgcc gttgctcggc gtcgttatcc ggcaccgcag cgctgagttc
     2461 gagcgtgccg aattcgacca gcggacgatc cgcgggccag tggctcgtcg agtcgtcaac
     2521 cacatcgccc tctgccgcca cctgaaccac aatgcgcaac ttcaccggcg cagccgcgag
     2581 gcggccgcgc aattcgtcga aaagaaaatt cggcgactgt tttttggcgg tttccgtgtc
     2641 cagatacgcc gtgccttcca cgggcagaat gcgatagcgg ccaaatttgc cgactccctc
     2701 ggcgttggtg aatttgtaag cgctgacaga aaaaaagttt tcgtgcgcga aactggtggg
     2761 aattggcttg ggcgcctgaa c
//
LOCUS       DASTPM010000565         6376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_942629, whole genome shotgun sequence.
ACCESSION   DASTPM010000565 DASTPM010000000
VERSION     DASTPM010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942629"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..707
                     /gene="fabZ"
                     /locus_tag="VFR42_09985"
     CDS             225..707
                     /gene="fabZ"
                     /locus_tag="VFR42_09985"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU0369530.1"
                     /translation="MIMDVVEIQKYLPHRYPFLMVDGILEIERLKRIVGVKNVSINEA
                     HFQGHFPGKPVMPGVLIIEAMAQTGGLLLLLEVPDRENKLLFFAAVDGARFRRPVVPG
                     EQLKIEMTVLAWRGDFCKLEGKALVDGQLAAEATLMCKMVDRDPGQKESGQKEKDASK
                     "
     gene            704..1489
                     /gene="lpxA"
                     /locus_tag="VFR42_09990"
     CDS             704..1489
                     /gene="lpxA"
                     /locus_tag="VFR42_09990"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU0369531.1"
                     /translation="MSGPEIHPSASVAASAKLARGVKVGAFAVVGEAVELGEDCLVHP
                     HAVIYGPSRCGAANIFHPFCVVGGDPQDYTFAGEDTELVAGDKNIFREYVTVSRGTKK
                     GGGVTRLGNENFLLAYTHIGHDCQIGSNNLFVNAATLAGHVTIEDFATIGALSPVHQF
                     CRVGRYAYVGASTVIVQDVPPFARIVTERQTKSFGPNSIGLERKGFSAERIKTLQRAF
                     RLLSRSKMNTSQALAELRAQFGESEDVRELVKFIEAAERGIVK"
     gene            1505..2389
                     /gene="lpxI"
                     /locus_tag="VFR42_09995"
     CDS             1505..2389
                     /gene="lpxI"
                     /locus_tag="VFR42_09995"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEU0369532.1"
                     /translation="MTTIAPKSNGWGLIAGNGRFPFLVLEGARSQGIDMAVIAIKEEA
                     DPQLAQQAQRVHWVSLGELSKTIDLLHQEGVGQAVMAGQVKHNKIFSSIRPDWKLAKL
                     LFALPRKNTDALIGAVAKVLEDEGIQLVDSTLFLKPLVPDAGVLSRRSPNDHEAADIA
                     YGVGVARHIAGMDIGQTVVISDQACVAVEAMEGTDETITRAARIASGKPLVVVKVSKP
                     KQDMRFDVPVVGLPTIAAMKAAGATALAVDAQRTLFFDRATLIAAADEAGIAIQAVPP
                     APATAMEPNTSPKGIEKK"
     gene            2386..3420
                     /locus_tag="VFR42_10000"
     CDS             2386..3420
                     /locus_tag="VFR42_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0369533.1"
                     /translation="MTAAAKPGIRVAVVGCGEFGRNHARVYREMECAQLAGAFDADPA
                     RAAAFAQEFHTQTFLSLQEMQGRVDAASVAVPTVAHAEVGSRLMEFGIDVLVEKPMAK
                     DLEGADALLAAAKKHARILQVGHVERFNPAVLAVEPILNRPLFFEVHRLGVFTPRSLD
                     VDVIYDLMIHDLDILLALVGEPVIEVKAVGIPVLSDKVDIAHARLEFSGGAVANVTAS
                     RVSTERVRKVRFFQQHEYISLDYARRDALRVSVRQPATEPGAQPEFVFEKLAAAGVEP
                     LRAELEAFLDAVRTRKEPRTNGMAGRAALELATRVMACIQEHAARVQPTGSHPAADLS
                     AFYHPRKDDK"
     gene            3417..4781
                     /locus_tag="VFR42_10005"
     CDS             3417..4781
                     /locus_tag="VFR42_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0369534.1"
                     /translation="MIPRSLVPFDARPSALQPSQRRRPSTLDDRTLVPAGLAPTPLET
                     KSSIPANLPLESIATRFVVPRDVNPEAYLIQEVSTLPPQPSELDERITVPAGAAPPEI
                     VPPPSRVSEELVEKDVFLTGEVNFLANQELREKQARKNLIDRAYSIVLHAAVLLLLIF
                     QPKLFNRRPPSAAEQEIARKQMVLLLPPGSFETAPPAPRVVTPPPKVKVDPRILRQIA
                     PPEPVAPPQPVQPKVQPKELPSAPAPQPNANQPPPAPVPKQEVATNNAPKLELPDTPT
                     ARPNLTLPKSSSNPLRDTLRDMSRQSNSSGTSGAFSAPTPRVPAGGGGRLGGGATQGN
                     SGMSGAYQILTPTEGVDFSDYMARVLASVRRNWYAVMPESAQLGDRGRVVLEFSIRRD
                     GSVPNEDPQRLLGSGKEPLDRAAVSAIRSSNPFDHLPPAFSGPEIRLRFIFLYNLPLD
                     AANQ"
     gene            4952..5896
                     /gene="miaA"
                     /locus_tag="VFR42_10010"
     CDS             4952..5896
                     /gene="miaA"
                     /locus_tag="VFR42_10010"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU0369535.1"
                     /translation="MTSLPPLLAIVGPTAAGKSSLGIFLAKELSGEVLACDSTQLYRG
                     FDIGTAKPSIADLQSIPHHLLDILEPTDASTAGDYRERAIAVLAHLRARQRLPILTAG
                     TGLYLRALLDGLGDLPQRSEEIRERLRASVAEHGQGHLHKMLEHLDSQAAKQIAAADE
                     QKLIRAIEICLVSGKPVSAVHRAGRTPLTGWRIFKIGLEPPREALYQRIHARTDSMLA
                     EGWLNEVRRLMAGGLPDNAKPFDFIGYRELRSHLRGELTFEQARAAIQQGTRQYAKRQ
                     LTWFRREANVHWLSGFGDAPDIQSMAQKWLQQAFSQEP"
     gene            5982..>6376
                     /locus_tag="VFR42_10015"
     CDS             5982..>6376
                     /locus_tag="VFR42_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369536.1"
                     /translation="MKRPQSISSTERALLVGVAWKRSPRVPGMTAGAPERESLAELVE
                     LARSAGASIAGTVFQLRDVADPATLVGRGKLDEIRAEASAKQAPLIIFDSDLSPVQQR
                     NIEEHTECRVIDRTQLILDIFARHARSREG"
BASE COUNT         1267 a         1981 c         1850 g         1278 t
ORIGIN      
        1 ggcgcaaata catcagcaag ctctttgcat cggggagttg aatcatggcg ccgatgctaa
       61 caaccccgcg ccgggctggc aaattcaaca cttctgcgcc gcagccggcc cgttcccgag
      121 tcacaatctg accgccggga ctgccactgc ctccggtata atgatagttt tggcggtgag
      181 cgcgctgccc gcagggtgtg cggcacagcg acggagaacc tcgaatgatc atggacgtgg
      241 tggaaatcca gaaatacctg ccgcaccgct atccgttttt gatggtggac ggcatactgg
      301 aaatagaacg cctgaagcgt atcgtcggcg tgaaaaacgt cagcattaac gaagctcatt
      361 ttcaggggca ttttccgggc aagccggtta tgccgggtgt gctgatcatc gaagccatgg
      421 cgcagaccgg tgggctgcta ttgctgctgg aagttcccga tcgtgaaaac aaactgctgt
      481 tttttgccgc ggtggatggc gcgcggtttc ggcggccggt ggtgcccggc gagcaattga
      541 agattgaaat gacggtgctg gcttggcgcg gcgacttttg caagctcgag gggaaggccc
      601 tggtggacgg ccagttggcg gcggaagcaa cgttgatgtg caagatggtg gatcgcgatc
      661 ctggtcagaa agaatcgggt cagaaagaga aagacgccag taaatgagcg gtccggaaat
      721 tcatccgagc gcgtcggttg cggcttcggc aaagcttgcg cgtggcgtca aggtgggagc
      781 gttcgccgtt gttggggaag cagttgagct gggcgaagat tgcttggttc atccacatgc
      841 ggtgatttac gggccgtcgc gttgcggcgc ggcaaacatt tttcatccct tttgtgtggt
      901 gggcggcgat ccccaggact atacgtttgc gggagaggac acggagctcg tcgcgggcga
      961 caagaatatt tttcgcgaat atgtgaccgt cagccgcggc accaaaaaag gcggcggagt
     1021 gacgcgcctg ggcaacgaaa attttctgct ggcctacacg catatcggcc acgattgcca
     1081 gatcggcagc aataatttgt ttgtgaatgc ggcgacgctc gcgggtcacg tgacgattga
     1141 ggattttgcg acgatcggcg cactgtcgcc ggtacaccaa ttttgccgcg tcgggcgtta
     1201 tgcctacgtc ggcgcttcga cggtcattgt gcaggacgtg cctccatttg cgcgcatcgt
     1261 cacggagcgc caaaccaagt cgttcggacc caactccatc gggctggagc gcaaaggctt
     1321 ttcggcagaa aggatcaaga cgttgcaacg cgcctttcga ctgctgtcgc ggtcgaagat
     1381 gaacacttcg caggcgctgg cggagttgcg cgcgcagttt ggcgagtccg aagacgtacg
     1441 cgagttggtg aaatttattg aggccgccga gcgcggcatt gtgaagtaag gcagccttcc
     1501 cgagatgact acaatcgcgc caaaaagcaa cgggtgggga ctcatcgccg gcaacgggcg
     1561 ctttccgttt ctggtgctgg aaggcgcgcg cagtcaaggc atcgacatgg cggtaatcgc
     1621 gattaaagaa gaagctgatc cgcaactggc acaacaagcg cagcgcgttc attgggtgag
     1681 tctcggcgaa ctttccaaaa cgattgacct actgcatcaa gagggtgttg ggcaggccgt
     1741 catggccggg caggtgaagc acaacaaaat tttcagctcg atccggccgg actggaagct
     1801 ggccaagttg ctctttgcgc tgccgcgaaa aaataccgac gcgctgatcg gcgcagtagc
     1861 aaaggtgctg gaggacgaag gcattcagct ggtggattca acacttttct taaagccgct
     1921 cgtgcccgac gccggcgtgc tgtcgcggcg ctcgccgaac gatcacgagg ccgccgacat
     1981 cgcctatggc gtgggcgtcg cgcgccatat cgccggcatg gacattggcc agaccgtggt
     2041 gatttccgat caagcttgtg tcgcggtgga agcaatggaa ggtaccgacg aaacgattac
     2101 gcgcgcggct cgcatcgcgt caggcaagcc gctcgtagtg gtgaaggtca gcaagcccaa
     2161 acaagacatg cgtttcgacg tgccggtggt tggcttgccc accatagcgg ccatgaaagc
     2221 cgcaggcgcg accgcacttg cagtcgatgc gcagcgtacg ttgttcttcg atcgcgcgac
     2281 gctcatcgcg gcggcggatg aagcaggcat cgccattcag gccgttccgc cggctccggc
     2341 cacggccatg gaaccaaaca cttcgccgaa gggaatagaa aagaaatgac ggccgccgcc
     2401 aaaccgggta ttcgtgtggc cgtcgtgggc tgcggggaat ttggacgcaa ccacgcgcgc
     2461 gtgtaccggg aaatggagtg cgcgcaactc gccggcgctt tcgacgccga ccccgcccgc
     2521 gcagccgctt tcgcgcaaga atttcacacc caaacgttcc tttccttgca ggaaatgcag
     2581 ggacgagtcg acgcggccag cgttgccgtt cccacggtcg ctcatgccga agtgggcagc
     2641 cgcctcatgg aattcggtat cgacgtgctc gtggaaaagc cgatggcgaa ggatctagag
     2701 ggcgccgatg cgttgctggc cgccgccaag aaacatgcgc gcatcttgca agtcggccac
     2761 gtcgaacgtt tcaatcccgc tgtactggcc gttgaaccga tcctcaaccg cccgctcttt
     2821 ttcgaggtgc accgtctggg cgtttttacg ccgcgcagtc tggatgtgga tgttatctac
     2881 gacctgatga ttcacgacct ggacattctg ctcgcgctgg ttggcgagcc ggtgatcgaa
     2941 gtcaaagccg tcggcatacc cgtgctcagc gataaagtgg acatcgccca tgcgcggctt
     3001 gaattttccg gaggcgccgt cgccaacgtg acggcgagcc gcgtttccac cgaacgtgtc
     3061 cgcaaagtac gcttctttca gcagcacgag tatatttcgc tggattacgc gcgccgcgat
     3121 gctctgcgcg tcagtgtcag gcagccggca accgagccgg gcgcccaacc cgaatttgtg
     3181 tttgaaaaac tggcggcggc gggcgtcgaa cccttgcgcg ccgaactgga agcatttctc
     3241 gacgcagtgc gcactcgcaa ggaacccagg accaacggca tggcggggcg tgccgcgctc
     3301 gaattagcca cccgcgtcat ggcgtgcatc caggagcacg ccgcgcgcgt ccaacctaca
     3361 ggcagccatc cggcggcgga tttgtctgcg ttttaccatc cgagaaaaga cgacaaatga
     3421 ttccgcggtc cctggtaccg ttcgatgctc gcccatccgc gctgcagcct tcgcagcgcc
     3481 gccgcccctc cacgcttgat gaccgcacgc tggttccggc cggtttggcg cccacgccgc
     3541 tggaaaccaa atccagcatt cctgccaatc ttccgctcga atcgatcgcg acgcgctttg
     3601 tggtgccgcg cgacgttaat cccgaagctt atctgatcca ggaagtgtcg acgctgccgc
     3661 cgcagccatc tgagctcgac gaacgcatca ctgtgcccgc aggtgccgcg ccgccggaaa
     3721 tcgttccgcc tccgtcccgc gtctccgaag agctggtcga aaaagacgtc tttctaaccg
     3781 gcgaggtcaa cttccttgcc aaccaagagt tacgcgaaaa acaagccaga aaaaatctga
     3841 tcgaccgcgc gtattcgatc gtgttgcacg ccgcggtttt gctgctgctg atttttcagc
     3901 cgaagctctt caaccgccgg cccccaagtg ccgccgagca ggaaattgcc cgcaaacaga
     3961 tggtgctgct gctgccgccc ggctcgtttg aaacggcgcc acctgctccg cgcgtcgtca
     4021 ctccgccacc caaagtaaag gtagacccgc gtattttgcg ccagatcgct cctcctgaac
     4081 cggtcgctcc gccccaaccc gttcagccca aggtccagcc caaagaactt cccagcgcac
     4141 cggctccgca gccgaatgcc aatcagcctc ctcccgctcc cgtgccgaag caggaagtag
     4201 caaccaacaa cgcgcccaaa cttgaattgc ccgatacgcc gacggcgcgg cccaatctca
     4261 cactgccaaa atcgtcttcg aatccactgc gcgatacctt gcgagatatg tctcgacagt
     4321 ccaactccag tggcacatcc ggcgcttttt ctgctccgac cccgcgtgtt ccagccggcg
     4381 gaggtggacg tcttggtggc ggcgctacgc aaggcaatag tggcatgagc ggtgcgtatc
     4441 agattctcac gccgaccgaa ggcgtcgact ttagcgatta tatggctcgc gtcttggcca
     4501 gtgttcgccg caactggtac gcggtcatgc cggaatccgc gcagctgggc gatcgcggcc
     4561 gcgtcgtcct cgaattcagc atccgccgcg acggaagcgt gcccaacgaa gatccgcagc
     4621 gcttgctcgg ctccggtaag gaaccgctcg accgcgctgc cgtctccgcg attcgctcct
     4681 ctaatccttt cgaccacttg ccgcccgcct tcagcggccc ggaaatccga ctgcgcttca
     4741 ttttcttgta caatctgcct ctcgatgccg caaaccagta agccgaactc cgggatcttg
     4801 gcaacccgta agtgcaagga tcgtcggtaa gtctcgctgc ttgcggactg gcaagtatga
     4861 cggccatacg tcaacaagtt cttggcaaca tcctcctcgc ggcgatcgcc ctggttatct
     4921 ttgttgcgcg cacctggcat attctctttc gatgacgtct cttcccccgc tgctggccat
     4981 cgttggccct accgctgccg gcaaatcctc tctcggtatt tttctggcaa aagaattatc
     5041 cggcgaagta ctcgcatgcg attccacgca actctatcgc ggcttcgaca tcggcacggc
     5101 caagccttcc attgctgacc tccaatctat tccgcatcat cttttggata ttctcgaacc
     5161 gacggacgct tccaccgccg gcgattatcg cgaacgcgcc attgcggtcc tcgcgcacct
     5221 acgcgcgcgt cagcgcctcc ccattcttac cgcgggaacc ggcctttacc tgcgtgcact
     5281 gctcgacggc ctaggcgatc tcccgcagcg ttctgaggag attcgcgagc ggctgcgcgc
     5341 ttcggtcgcc gaacacggcc agggccactt gcacaaaatg cttgagcacc ttgattcgca
     5401 ggccgcaaaa caaatcgcag ccgccgacga acaaaagttg atccgcgcca tcgaaatttg
     5461 ccttgtctct ggaaagccag tctccgcagt gcaccgcgcc gggagaactc cgctcaccgg
     5521 ctggcgcatc ttcaaaatcg gtctcgagcc gccgcgcgaa gccctttacc aacgcattca
     5581 tgcacgcacc gattccatgc tggcggaggg ttggctcaac gaagtccgcc gactcatggc
     5641 cggcggctta cctgacaacg ccaagccctt cgacttcatc ggctatcgtg aactccgttc
     5701 ccatctccgt ggggaactca catttgagca ggctcgtgcc gccattcagc aagggactcg
     5761 ccagtacgcc aaacgccagc tcacctggtt tcgccgcgag gcgaacgtgc actggctcag
     5821 cggttttggc gacgccccgg acatccaatc gatggctcag aaatggcttc agcaggcttt
     5881 ttcgcaagaa ccgtaatgtt catacacccc aggctcgtcc ggcgtatact cgctttgatg
     5941 accccgttct caacaattcg cgtctcttcg tccttgcttt catgaaacgt ccgcaatcca
     6001 tttcttccac cgagcgggcc ctgctggttg gcgtggcctg gaagcgctct ccgcgcgtcc
     6061 caggcatgac tgccggcgcg cccgaacgcg aatccctagc cgagctggtc gagctagctc
     6121 gcagtgccgg agcctctata gccggaacgg ttttccagtt gcgtgatgtc gcagatcccg
     6181 ccacgcttgt cggccgcggc aagctcgacg agattcgcgc cgaagcttcc gccaagcaag
     6241 ctccgctgat catcttcgac agcgatcttt ctccggttca gcagcgcaac atcgaagagc
     6301 acaccgaatg ccgcgtcatt gatcgcacgc agcttattct tgatatcttt gcccgacacg
     6361 cgcgcagccg tgaagg
//
LOCUS       DASTPM010000566         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_856315, whole genome shotgun sequence.
ACCESSION   DASTPM010000566 DASTPM010000000
VERSION     DASTPM010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856315"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFR42_10020"
     CDS             <1..290
                     /locus_tag="VFR42_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369537.1"
                     /translation="YQVLNVMSTAGASILALGYLLPMIYLTWSMRYGKVAGKNPWPAT
                     GLEWTTDSPPLTENFHETPVVDWEPYDFLRRPELGLLGATSKKPVPAHGDD"
     gene            350..1120
                     /locus_tag="VFR42_10025"
     CDS             350..1120
                     /locus_tag="VFR42_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEU0369538.1"
                     /translation="MHRTGELYGQFQTLEQQKESATLGMWVFLITEILFFGGVFMAYT
                     INRHAYPTAFGVGGNMLDLKLGFFNTVVLIASSFTMAMSVWSAQTSRKKAVPAWLIAT
                     LILGSVFLGIKVVEYKQKFDHHLIPGHNFDVTYCSRNQVACGLDDKDLAKEQKEIREG
                     IEAERFPGASEEEATVALNAHSQLYFSLYFGMTGLHALHMVIGAGLLLWLIIEGLKGR
                     FDANYNTPVENIGLYWHFVDIVWIFLFPLLYLINRHLA"
     gene            1140..1499
                     /locus_tag="VFR42_10030"
     CDS             1140..1499
                     /locus_tag="VFR42_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEU0369539.1"
                     /translation="MAEHKEHIVSPKIYIVVLTALLLGTGVTVWAAYIDLGRWNIVLA
                     LTIAVTKMTLVILFFMHGKYSSRRTKLIIASGFFWLAIMLVLTLADYTTRHPEPSRSQ
                     LAPVPTLQSSAGSALSA"
     gene            2014..>3166
                     /locus_tag="VFR42_10035"
     CDS             2014..>3166
                     /locus_tag="VFR42_10035"
                     /inference="COORDINATES: protein motif:HMM:NF036484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369540.1"
                     /translation="MGLTKLVVRGARQHNLKNISIEIPRNTLTVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDQMERPEVDVIEGLSPAIAIEQKTTTRSPRSTVGT
                     ITEIYEYLRVVYASVGVPHCPSCGNPIKRQSSEAIVQAILGGELCKPDDRVMILAPIV
                     RGRKGAYRKELEKLAQDGYVRVRINGELFPLDDPPQLDKRKNHTIEVVVDRLLVKQGI
                     ASRLEQSIAASLKLASGLVTVAVVGGQEFTFSEKLACPDCGISVPQLEPRSFSFNSPY
                     GACPACNGLGSKYDFDPAKVVTDWTRPLFDGGLGPGSGSAILKRTIELAAYAHGFDLD
                     TPFEKFPVKTQTLILYGYPPVGAPGYKSNGDAPKAQGKADKGFRFQGVLK"
BASE COUNT          641 a          952 c          840 g          733 t
ORIGIN      
        1 agtaccaagt gctaaatgtc atgtccacgg cgggcgcttc gattctggct ctgggctact
       61 tgctgcccat gatttatctg acctggtcca tgcgctacgg caaagtggcc ggcaagaatc
      121 cctggcctgc caccggtctg gaatggacca ccgattcgcc gccgctcacg gagaatttcc
      181 acgaaacgcc tgtggtggat tgggagccat acgatttctt gcgtcggccg gagctgggcc
      241 ttttgggcgc cacttccaag aagcccgtgc cggcgcacgg agacgattga ttaaggcctt
      301 gtagctggca gttttaggct gctacttgtt cacgaagagg agtcaccgcg tgcatcgcac
      361 cggagagctg tacggacaat ttcagacgct ggagcaacag aaggaatccg ccacgctggg
      421 catgtgggtc tttctgatca ccgaaattct tttcttcggt ggcgtcttca tggcctacac
      481 catcaaccgc cacgcttatc ccacggcctt cggtgtcggc ggcaacatgc tcgaccttaa
      541 actcggtttc ttcaacacgg tcgttttaat tgccagcagc tttacgatgg cgatgtccgt
      601 ttggtcggcg cagaccagcc ggaagaaagc tgttcctgcg tggctcatcg cgacgctcat
      661 actaggttcc gtcttccttg gcattaaagt agttgagtat aagcagaagt tcgaccacca
      721 tttaatcccc ggccacaact ttgacgtcac gtactgctcc cgcaatcagg tagcgtgtgg
      781 tttggatgat aaggaccttg ccaaggaaca gaaggaaatt cgagagggga ttgaagcgga
      841 aaggttccct ggcgcgtcgg aagaagaggc cacggtcgcc ctcaacgctc actcccagct
      901 ctatttttcg ctctacttcg gcatgaccgg gcttcacgcc ctgcacatgg tcataggagc
      961 cgggctgctg ctatggctca ttattgaagg acttaaaggc aggttcgacg ccaactacaa
     1021 tacgccagtg gaaaacatcg gcttgtactg gcactttgtc gatatagtct ggattttcct
     1081 attcccgtta ctgtacctca tcaaccgtca cctagcctga ggcatgagcg aggaaacgcg
     1141 tggctgaaca taaagagcat attgtctcac cgaagattta catcgtcgtg cttaccgccc
     1201 tcctgttagg caccggcgtg acggtttggg ccgcctacat cgacctcggc cgctggaaca
     1261 ttgtgctcgc gcttactatt gccgttacaa agatgaccct ggtcattctc tttttcatgc
     1321 acggcaaata cagctcgcgc cgcaccaagc tcatcatcgc gtcgggattt ttctggcttg
     1381 ctatcatgct ggtgctcacg ctggccgatt acactacccg ccaccccgag cccagccgca
     1441 gccagctggc cccagtcccc acgttgcaaa gcagcgcagg ttcggctctg tccgcctaat
     1501 ttgcgtttct aatttcgatt ttcgatttcg ttttccgatc cgggcacacc tctgccgtgt
     1561 tcggtagttc ggggtgtttg gaaggagttg gtgtactcgg aggcgtccgg gacaccatcg
     1621 gcagaagtgc agttcggggt ctcgcaaccc cgaaaaatca aaaggggaga agtcagccgg
     1681 cccagccctc aatcccctaa agggatcctc ggctaactcc tcccacgctt tcggaaggat
     1741 gcaacctgct cgccattcct aagtccttct attgcaacac gttccttctg ggaacccttg
     1801 taaaaatgcg gtaccccctg cgcgatttta cgtgtcgggg atttcctgta ccgtcaaaac
     1861 ttcataaatt gacaggtacc gtaaagcggc tttcttgacc ccctgagcgc accgctgcta
     1921 ttgttaggag ttattctggc ctcagtgtcg ttactgggtg ccggtgctcg ccttctgcgg
     1981 cgggcaggct cctgcggagg gagccttcgc gatatgggcc tgaccaaact tgtcgtccgc
     2041 ggcgctcgcc agcacaacct gaagaacatc agcatcgaaa tcccgcgcaa tacccttacg
     2101 gtgatcaccg ggctgtccgg ttccggcaag tcttccctgg cgttcgatac gatttacgcc
     2161 gagggccagc gccgctacgt cgaatcgctt tccgcttacg cccgccagtt tctcgatcag
     2221 atggagcggc ccgaagtgga tgtcattgaa gggctttccc cggccatcgc catcgaacaa
     2281 aaaaccacca cgcgctctcc gcgttcgact gttggcacca tcaccgaaat ttacgagtac
     2341 ttgcgcgtgg tgtacgcctc ggttggtgtt ccgcattgcc cgagttgcgg caatcccatc
     2401 aagcgccagt ccagcgaagc tatcgtgcaa gccattctcg gcggcgaact ctgcaagcct
     2461 gatgatcgcg tcatgatcct tgcgcccatc gtgcgcggcc gcaaaggcgc ttatcgcaag
     2521 gaactcgaga agctggctca ggatggctat gttcgggtgc gcatcaacgg tgaacttttt
     2581 ccgctggatg atccacccca gctcgacaag cgcaaaaatc atacgatcga agttgtcgtg
     2641 gaccggttgc tcgtcaagca aggcatcgcg tcgcgcctcg aacagtccat tgctgcctca
     2701 ctgaagctcg cttccggctt ggtgacggta gccgtcgtcg gcggccagga atttaccttc
     2761 tccgaaaaac tggcctgccc cgattgcggc atcagcgttc cgcaacttga gccgcgctcc
     2821 ttcagcttca attcgcccta tggcgcatgc ccggcgtgca acggcctcgg ttcgaaatac
     2881 gatttcgatc ccgccaaagt tgtgacggat tggacgcgcc ctcttttcga tggcggtctc
     2941 ggccccggtt ccggctccgc cattctgaaa cgtacgatcg aactcgcggc ctacgcgcac
     3001 ggtttcgatc ttgatacgcc gttcgaaaaa ttccccgtca aaacgcaaac cctgattttg
     3061 tacggctacc cgcccgtggg cgcgcccggc tacaaatcca acggcgacgc gccaaaagcc
     3121 caaggcaaag cggacaaagg cttccgtttt cagggcgtcc tgaaat
//
LOCUS       DASTPM010000567         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_423890, whole genome shotgun sequence.
ACCESSION   DASTPM010000567 DASTPM010000000
VERSION     DASTPM010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423890"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFR42_10040"
     CDS             complement(<1..627)
                     /locus_tag="VFR42_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0369541.1"
                     /translation="MSTEILFLLLLGALVLANVALEFIARWRNPPIGEFLDVDGVRLH
                     FIIVGDPDAPPLVMFHGNGTLLQDLTISGLVDAAASKFRVICFDRPGFGHSSRPRSVI
                     WGPERQAELFCAALTRLGIERPLVLGHSWGTLVALAMAARNSERVKGLVLVSGYYFPT
                     WRFDVWFASIAAIPVIGAALRYTVSPISSWLGLPLFAKKTFAPKPVPEI"
     gene            939..1433
                     /locus_tag="VFR42_10045"
     CDS             939..1433
                     /locus_tag="VFR42_10045"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEU0369542.1"
                     /translation="MARETEFAQQTGERAMQAAAFGFTWSREFAEESFNQGKRTLDAL
                     MRITRKMADDFESQCSAIREQSTEVTEKTLSNAMEYGQKLARVKEPQEFAQCQSEFLA
                     RQAQTLADQTKEFGLRMQKAAQTFASNASNAMAEASRRTEDTVSTITSRAADQTSKRH
                     RAEA"
     gene            complement(1817..2140)
                     /locus_tag="VFR42_10050"
     CDS             complement(1817..2140)
                     /locus_tag="VFR42_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0369543.1"
                     /translation="MPKITYIEHDGAQHTVEAELGATVMETALRNDIASIVAECGGSC
                     TCATCLVHVDEKWFEVVGPPSGEEEEQLDAAFEVKPTSRLSCQIKVTEALDGLVVHTP
                     SYQGR"
     gene            complement(2282..>2541)
                     /locus_tag="VFR42_10055"
     CDS             complement(2282..>2541)
                     /locus_tag="VFR42_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369544.1"
                     /translation="IAEFGLNLTENLIAVDTAKFQSTTPGIFAIGDINTYPGKLKLIL
                     SGFHEAALMSQKAFHYVFPEKRLVFQYTTSSTSLQKKLGVA"
BASE COUNT          547 a          742 c          799 g          453 t
ORIGIN      
        1 gatctccggc accggtttgg gtgcgaaggt tttcttcgcg aaaagcggaa ggccgagcca
       61 gcttgaaatc ggagaaacgg tgtagcgcag ggcagcgccg atgaccggga tggcggcgat
      121 gctcgcgaac cagacgtcga agcgccaggt cgggaagtag tagccggaga ccaggacaag
      181 ccccttcacg cgctcgctgt tgcgcgccgc catcgccagc gcaaccaggg tgccccacga
      241 gtggccgagc accagcgggc gctcgatgcc gagccttgtc agcgccgcgc agaagagctc
      301 ggcctggcgc tccgggcccc agatgacgct gcgcggccgg ctgctgtggc cgaatcccgg
      361 gcgatcgaag cagatgacgc ggaatttgct cgccgcggcg tccaccagcc cgctgatggt
      421 gagatcttgc aggagtgttc cattgccgtg gaacatgacc agcggcggcg catcgggatc
      481 gccgacgatt atgaaatgca atcgtacgcc atcgacgtcg aggaactcgc cgatcggcgg
      541 attgcgccag cgcgcaatga actcgagcgc gacattcgcg agtaccagcg cgccgagcag
      601 caagagaaat aaaatctccg tgctcatgtg acgcagtcct cccatgccga aatgctatgc
      661 gaaaacgagc aggatcggcc ggcaagaaag aaagttgcgg cggcttccgc cgaaaatcgc
      721 gcgctttgac ggaatttccg cggcgctgga cgaacgccgc gaacttgcgc gcgttccgct
      781 caaaaataga aacagcggtg acttagtttt accaaattgg aggaacagga catgcatgag
      841 accgttggcc aagtgacggc acaagcgtcg tcaatccaca catgtcaaaa gttatacgtg
      901 cacaacatcc acgtgggatg gaaagggaga aggatataat ggctagagaa acggaattcg
      961 cgcagcaaac cggcgaacgc gcaatgcagg cggcggcatt cggatttacc tggagccggg
     1021 agttcgcgga agaaagtttc aaccagggca agcggacact cgacgcgctg atgcggatca
     1081 cgcgcaagat ggcggacgat ttcgagagcc agtgttccgc gatccgcgag cagagcaccg
     1141 aggtgaccga gaagacgctg tcgaacgcga tggaatacgg acagaagctc gcacgcgtga
     1201 aggaaccgca ggagttcgcg cagtgccaga gcgagttcct tgcccggcaa gcgcaaacct
     1261 tggccgacca gaccaaggaa ttcggcctgc gcatgcagaa ggcggcgcag accttcgcca
     1321 gcaatgcatc gaatgcgatg gctgaagcaa gccgccggac cgaggacacg gtctcgacca
     1381 tcacctcgcg cgcggcggac cagacttcga agcgccatcg cgccgaggcg taagcgctca
     1441 ttagcggcgt taagttgaac gccgttcatc cccgcgcaag cggggatcca gagagatatt
     1501 gtttctgggt ccccgctttc gcggggacga acgggttttt tgttgatgca ccacattcta
     1561 aggcgtcatg gccggccttg tgccggccat ctcgcttagt gaggcactgt gccttcatag
     1621 gcgggatcac cgggacaagc ccggtgatga caaatggagg ggaaagtcgc ggcgaaaccc
     1681 ccggccggcc cacttaaagt gaagccttag tccgcttgaa gtgaagcctt aagtggctct
     1741 gtcaccgtag gtttaagtgc tattaagaca aagttaacgg gccaagtcct cgccgataca
     1801 tcgtcccgag tggtgctcac cgcccctgat acgatggcgt gtgcacgacc agcccatcga
     1861 gcgcctcggt caccttgatc tggcaggaga ggcgcgaggt cggcttcacc tcgaaggcgg
     1921 cgtcaagctg ctcttcctct tcccccgagg gcgggccgac aacctcgaac catttctcgt
     1981 cgacatgcac caggcaggtg gcgcaggtgc acgagccgcc gcattctgcc acgatgctgg
     2041 cgatgtcgtt gcgcagcgcg gtctccatca ccgtcgcgcc gagctcggct tccaccgtgt
     2101 gctgcgcgcc gtcgtgctcg atgtaggtga tcttgggcat tgggaccaga accacactcc
     2161 gttcattccc gcgcacgcgg ggatccaggc gacgctttta tttgtgcccc tgggtccccg
     2221 ctttcgcggg gatgaacgag aaaagcttta gctacgctgg ttcgcattct ccgcatcgga
     2281 ctcacgccac gccgagtttc ttctgcaggc tggtggacga tgtcgtgtac tggaacacca
     2341 gcctcttctc cggaaagacg tagtggaatg ccttttgcga catcagcgca gcctcgtgga
     2401 agccggaaag gatcagcttg agcttgccgg gataggtgtt gatgtcaccg atggcgaaga
     2461 tgcccggtgt cgtgctctgg aatttcgccg tgtccaccgc gatcagattt tcggtgagat
     2521 tcaacccgaa ttcggcgatc g
//
LOCUS       DASTPM010000568         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1029319, whole genome shotgun sequence.
ACCESSION   DASTPM010000568 DASTPM010000000
VERSION     DASTPM010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029319"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /gene="nusB"
                     /locus_tag="VFR42_10060"
     CDS             complement(<1..385)
                     /gene="nusB"
                     /locus_tag="VFR42_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU0369545.1"
                     /translation="MSLRSKSREFAMQMLFQWDMSQQDPAKLEAKFWRGAKAADNTRA
                     FANRLFEGAAKEVLVLDEIIVKHAENWRFDRLAAIDRAILRLAMHELRSSDTPPKVVL
                     NEAVDLAKKFSGEDSGAFVNGILDAF"
     gene            complement(387..929)
                     /gene="ribH"
                     /locus_tag="VFR42_10065"
     CDS             complement(387..929)
                     /gene="ribH"
                     /locus_tag="VFR42_10065"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU0369546.1"
                     /translation="MATKLQPAPIDGQLTASGFRFGIVVSRFNSFITERLLSAAVDAL
                     ERAGAVSDSVDIVHVPGAFELPLAAKKLAATGRYHALIAIGCVLRGETTHYDYVCSET
                     SRGLQLAQMDTGLPIIFCVLTCDTLEQAIDRAGLKGGNKGFEAGLAAIEMAHLSRKLS
                     AGNSSSAPAKTSSSRKPRRK"
     gene            complement(940..1722)
                     /locus_tag="VFR42_10070"
     CDS             complement(940..1722)
                     /locus_tag="VFR42_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0369547.1"
                     /translation="MAYENILFEKKDGIAYITFNRPKVLNALNRKTIEELRDALLDAR
                     DDVSVRVLILTGAGEKSFVAGADISELAQRTPVDGKDFSLFGQSVFHQLETLGKPSIC
                     AINGFALGGGCEMALCCTIRLASKGAKLGQPEVKLGIIPGYGGSQRLARLCGKGVAHE
                     LCLTGEMITAEEAQRIGLVNHIYEPAELLPAAEAMAKKIIANAPLAVKYTIEAIERGV
                     EMPQEEGLFLEATLFALSCATEDMREGTKAFLEKRKADFKGK"
     gene            complement(1738..>2025)
                     /locus_tag="VFR42_10075"
     CDS             complement(1738..>2025)
                     /locus_tag="VFR42_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0369548.1"
                     /translation="DAIRALEEGVGSIEDIDNSMKLGCGHPMGPLTLLDFVGLDTTYY
                     IANIMFEEFRERRFASPPLLKRMVLAGWNGRKAGRGFYDYSDPQKPKAMKF"
BASE COUNT          394 a          574 c          620 g          437 t
ORIGIN      
        1 ggaaggcgtc gagtatgccg ttgacgaacg cgcctgaatc ttcgccggag aatttcttgg
       61 ccaagtccac cgcttcgttg agcaccactt tgggcggcgt gtccgacgag cgcagttcgt
      121 gcatggccag gcgcagaatg gcgcgatcga tggcggcgag ccggtcgaag cgccagtttt
      181 ccgcgtgctt cacaataatt tcgtcgagca ccagcacttc tttggcggcg ccttcgaaca
      241 aacggttggc gaaggcccgc gtgttgtccg cggctttggc gccgcgccag aattttgctt
      301 ccagcttggc cgggtcctgc tggctcatgt cccattgaaa gagcatctgc atggcgaatt
      361 cgcgggattt gctgcgcagc gacatcttat ttgcgccgcg gctttcggga agaagaagtt
      421 ttggctggag cggaggaaga attgcccgcg ctcagcttgc gcgagagatg ggccatctcg
      481 atggcggcca gaccggcttc aaaacctttg ttaccgcctt tgagcccggc gcgatcgatg
      541 gcttgctcca gagtgtcgca ggtcaaaacg cagaaaatga tcggcaggcc tgtatccatt
      601 tgcgcgagct gcaaaccgcg cgacgtttcc gagcagacgt aatcgtagtg ggtggtttcg
      661 ccgcgcagta cgcaacctat ggcgattagc gcgtggtagc ggccggtggc ggcaagtttc
      721 ttcgcggcga gaggtagttc aaaagcgccg ggaacatgga cgatgtcgac gctgtcgctg
      781 acggcgcctg cgcgctccaa ggcatccacg gcggcggaga gcagacgctc ggtgataaaa
      841 ctattgaaac ggctgaccac aatgccaaag cgaaaaccgg atgcggtaag ttggccgtcg
      901 ataggggcgg gctgaagttt tgtggccatc gcgtgtgtgt tatttgccct tgaagtccgc
      961 ttttcttttt tccaggaaag ccttggtgcc ctcgcgcata tcttccgtgg cgcaggatag
     1021 cgcgaacagg gtggcctcca ggaacaagcc ttcttcctga ggcatttcca cgccgcgctc
     1081 gatggcttcg atggtgtatt taactgcgag cggagcgttg gcgatgattt ttttggccat
     1141 ggcttcggcg gcgggcagaa gctcggccgg ttcgtaaatg tgattgacca gcccgatgcg
     1201 ctgcgcttct tccgcggtga tcatttcgcc ggtgaggcaa agctcgtgcg cgacaccttt
     1261 accgcacaga cgcgccaggc gctgcgagcc gccatagccg ggaatgatgc caagtttcac
     1321 ttccggctgg ccgagcttgg cgcccttgct ggccaggcgg atggtgcagc aaagcgccat
     1381 ttcgcaaccg ccgcccagag caaatccatt gatcgcgcaa atcgaaggct tgcccagcgt
     1441 ttccagttgg tgaaacacgc tctgcccgaa gagcgaaaaa tccttgccat ccaccggggt
     1501 gcgctgggcc aactcgctga tgtccgcgcc ggccacaaac gatttctcgc ccgcgccggt
     1561 aagaatcagc acgcgcacgg aaacatcgtc gcgcgcatcg agcagcgcgt cgcgcaattc
     1621 ttcgatcgtt ttgcgattga gcgcgttcag cactttcggg cggttgaagg tgatgtaagc
     1681 gatgccgtct tttttctcga aaagaatatt ttcgtaggcc atattcacgt cacaatctca
     1741 gaatttcatc gctttgggct tttgcggatc ggagtaatcg tagaagccgc gcccggcttt
     1801 gcggccgttc catccggcca gcaccatgcg cttgagcagc ggaggcgaag caaagcggcg
     1861 ctcgcgaaac tcctcgaaca tgatattcgc gatgtagtag gtagtgtcca agccgacaaa
     1921 atcgagcagc gtcagcggcc ccatgggatg cccgcagccc agcttcatgg aattgtcgat
     1981 gtcttcgatc gagccgaccc cttcttccag cgcgcgaatg gcatc
//
LOCUS       DASTPM010000569         5665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_623487, whole genome shotgun sequence.
ACCESSION   DASTPM010000569 DASTPM010000000
VERSION     DASTPM010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623487"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFR42_10080"
     CDS             <1..544
                     /locus_tag="VFR42_10080"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU0369549.1"
                     /translation="ARALLYYAIALPGLATVKLVVPAFYSTKDTKTPVIVAAISMVLN
                     VVLNLVFIEFFFKRVQNGGPALATALACYFDFFALLLIFRLRYGVMGMREIARSFAKI
                     ALCSGIMGVACWLGAHYTEFTIHSRFLVQLLVFTGLIVGATALYLALAWIFRCSEIEE
                     IYGIATRRKRGGAEYVESEG"
     gene            544..1443
                     /gene="xerD"
                     /locus_tag="VFR42_10085"
     CDS             544..1443
                     /gene="xerD"
                     /locus_tag="VFR42_10085"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU0369550.1"
                     /translation="MELAAAIAGFLTHVQVEKGLASNTQSAYRTDLAKFEDFAKKRKV
                     ALEAVNQDHLVDFLAGLYRQKLESKTVARHLVSLRNFFRYAQVQELITEDPTAHLESP
                     KIRRSLPGYLRLEDVEKLLEQPDQKTPLGLRDRAMLEVLYSTGLRVSELTSLGVTDLD
                     TKVGCVRCIGKGDKERIVPVGRKALGIVERYLRDGRPQLVKAGRGSAGSYLFVNRRGG
                     RISRVGVWKILSAYGRRAGLRVALTPHMLRHSFATHLLERGADLRSVQLMLGHSDIST
                     TQIYTHVVEERLKQIYKAHHPRA"
     gene            1516..2967
                     /locus_tag="VFR42_10090"
     CDS             1516..2967
                     /locus_tag="VFR42_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369551.1"
                     /translation="MPDYMFLLESRLLPEQRAAMMRVQELSAALGFNVYLTGGTVRDL
                     ITGASLRDLDFTVEGNPSKIARELEKGGAKVLHEDEKLRHIEILFAGDCEGSISGARD
                     DHYVRPGTRPEIRWSTIMEDLRRRDFSLNAIAISLNPASRGLLLDPTNGLSDIERAEV
                     RALTIHSFTNQPVRLLRSLRYAARMGFKLEQRTEEWFALAMERDLHHSITPDDAGAEL
                     QALAKEENPTEALKAWEEHDLMEIVNPVLAKKKPDYEAIRRLSNVRDDLMMAGLRPRL
                     ATPMLLAILGKLKDREQTNVLGKLGLRSAEIDSISGFADAAEAVGKELAGKKLNAPID
                     SYKFLEKVPVEKLAYLLAETRNSTALSKIKAYLNKWRPLRTSIPLVRDELTKIGMPAG
                     PKFDAVVEQMFALQLNGKGKTPEEREKILRKLSGIKEPPKPKDTKGKDKKGAKHADKI
                     HGHVLAKPEAKPAVKPPAKPAGKPAPAKAKKKK"
     gene            3065..>5665
                     /gene="dnaE"
                     /locus_tag="VFR42_10095"
     CDS             3065..>5665
                     /gene="dnaE"
                     /locus_tag="VFR42_10095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU0369552.1"
                     /translation="MGQPEFVHLHVHTDYSLLDGACETGELLDEASRQKMPAVAITDH
                     GNLFAAANFFNEASKRDVKPIIGCEVYVAKGSRHDRGEKTAVNGADRNEDDPGSRGTN
                     HLVLLCENLKGYHNLIKLVSTGFLEGFYYKPRIDYDVLAKHHEGLIALSACLKGVVTE
                     AVVTEKYDQARENAYRLRDIFGKQNFFLEIQDQGLDIEKAVNPQLVRLSKETGIPLVA
                     TNDFHYLHHEDAHAQEVLLCIQTGKTMGDANRMKFATDQFYFKSAVEMAKVFGEIPEV
                     LRRTVDIASRCNVKIERIPNPFPEFKVPDGQTASSYFEKVVREGFATRVPYLERLAKA
                     GALANPLSEYERRLTSEIQMIQKMRYEGYFLIVWDFIHYARAQGVPVGPGRGSAAGSL
                     VSYALRITDVDPLQYNLLFERFLNPERVSMPDIDIDFCMRRRGELIEYVTQKYGRENV
                     AQIITFGTMAAKAAIKDVGRAMEIPYVEVDRLAKMVPTTLGIELKDALEEAPQLKSAV
                     NSDEKLKDLMNVALRLEGLSRHASTHAAGVVISPQPLTELVPIYKTNRDEITTQYDMN
                     ALERIGLLKMDFLGLTTLTVLQDAVRMVEQNRGVRVDIDNLTLDDADTYKLFSRGDTT
                     AIFQFESHGMRDILRRYQPTRIEDLTALNALYRPGPIQGGMIDDFINRKHGKTKVSYE
                     LPQLQDILQETYGVILYQEQVMQIANRLASFSLGEADILRRAMGKKKKEEMAAQRAKF
                     MAGCAKNKINEKKAEKIFNLMEEFAGYGFNKSHSCAYALLAYQTAYMKTHYPVEFIAA
                     LLTSETGNAEKAVKYINEARGMNIPILPPDVNESDLYFTPVGESIRFGLAAIKNVGEN
                     TAKAIRESKLS"
BASE COUNT         1300 a         1448 c         1814 g         1103 t
ORIGIN      
        1 cgcgagagcg ttgctgtact acgcgatcgc gctgccaggg ctggcaacgg tcaagctggt
       61 ggtgccggcg ttttattcga caaaagacac caagacgccg gtaatagtgg cggcgatctc
      121 gatggtgctc aacgtggtgc tgaacctcgt gtttatcgag tttttcttca agcgggtgca
      181 aaacggaggc ccggcactgg ctacggcact ggcttgctac ttcgatttct tcgcgctgct
      241 gctcatcttc cgcttgcgct acggggtcat ggggatgcgg gagatcgccc ggtcgtttgc
      301 caagattgcc ctgtgctccg gaattatggg agtggcctgc tggctgggag cgcactatac
      361 ggaatttacg attcattcga ggtttttggt gcagcttctg gtgtttaccg gattgattgt
      421 gggagctacg gcgctttatc ttgcgttggc atggatcttc cgctgcagtg aaatcgagga
      481 aatctacggc atcgcaacac gccggaagcg gggcggggca gagtacgtgg agagcgaggg
      541 ctgatggaat tagctgcggc cattgcgggc tttttgaccc acgtgcaggt agagaagggg
      601 ctggcgtcga atacgcagag cgcctatcgg accgatctgg ccaaatttga ggattttgca
      661 aaaaagcgca aggtggctct ggaagcggtc aaccaggacc acctggtgga ctttctggcc
      721 gggctatacc ggcaaaagct ggaaagcaag acggtggcgc gccatttggt gagcctgcgg
      781 aacttcttcc gctatgcgca ggtgcaggag ttgatcacgg aggaccccac ggcgcatctg
      841 gagtcgccga agattcggcg ctcgctgccg gggtatttgc ggctggaaga cgtggaaaag
      901 ctgctggagc agccggacca gaaaacgccg cttggattac gcgaccgggc catgctggaa
      961 gtgctatatt ccacaggttt gcgcgtcagt gaattgacca gcctgggagt tacggacctc
     1021 gatacgaaag tgggttgcgt gcggtgtatc ggcaaaggcg acaaggaacg catcgtgccg
     1081 gtgggccgca aggcgctcgg tatcgtagag cggtacctgc gggacggccg tccgcagctg
     1141 gtaaaagcag ggcgcggctc ggcgggatcg tatctgttcg tgaatcgccg cggcgggcgg
     1201 atcagccggg tgggcgtgtg gaagattctg tcggcctatg gaaggcgggc tggtttgcga
     1261 gtggctctaa ctccgcacat gttgcggcat agcttcgcga cgcacctgtt ggagcgggga
     1321 gcggacttgc ggtcggtaca gctgatgctg ggccattcgg acatttccac cacgcagatt
     1381 tacacccacg tggtggaaga gcggttgaag cagatttaca aggcgcacca tccccgcgca
     1441 tagcgggcgg gccaggtaca gaagtttatt ttgttcaggg tttgtgtgca caagctctga
     1501 aagccgagga gagcgatgcc agattacatg ttcttgctgg agagccggct gttgccggaa
     1561 caacgcgcgg caatgatgcg cgtgcaggag ctttccgcgg cgctagggtt caatgtgtac
     1621 ctgacgggcg gaaccgtgcg cgacctgata accggcgcgt cgctgcgcga cctggatttt
     1681 acggtggagg gcaacccatc gaagatcgca cgcgaactgg aaaaaggcgg cgcgaaggtg
     1741 ctgcacgaag acgagaagct gcggcacatc gaaattctat ttgcgggcga ttgcgagggc
     1801 agcatttccg gcgcgcgcga cgaccattac gtgcggccgg gaactcggcc ggagattcgc
     1861 tggtccacca tcatggagga cttgcgtcgg cgcgactttt cgctcaacgc cattgccatt
     1921 tcgctgaatc cggcttcgcg aggacttttg ctggacccca cgaacgggct ttcggacatt
     1981 gagcgggcgg aagtgcgggc gctaacgatc cacagtttta ccaatcagcc ggtgcggctg
     2041 ctgcgttcgc tgcgctatgc cgcgcgtatg ggattcaagc tggagcaacg cacggaagaa
     2101 tggttcgcgc tggcgatgga gcgcgatctg caccacagca tcactccaga cgacgccgga
     2161 gcagaattgc aggccctggc gaaggaagaa aaccccacgg aagcgctgaa agcctgggaa
     2221 gagcacgacc tgatggaaat cgttaatccg gttttggcga agaagaagcc ggattacgaa
     2281 gccattcgcc gattgagcaa cgtgcgcgac gacctgatga tggcgggctt gcggccacgg
     2341 ctggcgacgc cgatgctgct ggcgattctg ggaaagctga aggatcgtga gcagacgaac
     2401 gtgttgggca aactgggcct gcgctcggcc gaaattgatt ccatcagcgg atttgcggat
     2461 gcggcagagg cagttggcaa ggaactggca gggaagaagt tgaacgcacc gattgactcg
     2521 tacaagtttc tcgagaaggt gccggtagag aagctggcgt atttgttggc ggaaaccaga
     2581 aattcgacgg cgctttcgaa gattaaagcg tatctgaaca aatggcggcc actgcgtacc
     2641 agcattccgc tggtgcgaga cgagctgacg aagattggaa tgccggcggg tccgaagttc
     2701 gatgccgtgg tggagcaaat gtttgcgctg cagttgaatg gcaagggcaa gacgccggaa
     2761 gagcgggaaa agattttgcg gaagctttca ggaatcaagg agccgccgaa gcccaaggac
     2821 accaagggca aggacaaaaa gggcgcaaag cacgcagaca aaattcatgg ccacgttctg
     2881 gcgaagccgg aagcaaagcc agcggtcaag ccgccggcga aacctgccgg caaacccgcg
     2941 cctgcaaaag cgaagaagaa aaaataagcg cgaagatgtg ctgcggcgct ccgcgccaga
     3001 gtcctgttga aaacgcatga atgagcgtcg caaatttctg cgccgcgaat tacaatgaag
     3061 gttcatgggg cagcctgagt tcgttcacct tcacgttcat accgattaca gtctgctaga
     3121 cggagcttgc gaaaccggcg agttgctgga tgaggcgtcg cgccagaaga tgccggcggt
     3181 ggcgattacc gaccacggca acctttttgc ggcggcgaac tttttcaacg aggccagcaa
     3241 gcgcgacgtc aagcccatca tcggctgcga agtgtatgtg gcgaagggca gccggcacga
     3301 tcgcggcgag aaaactgctg taaatggcgc ggaccgcaat gaagatgacc ccggctcgcg
     3361 cggcaccaat catctggtgc tgctgtgcga gaacctgaag ggttaccaca acctgatcaa
     3421 gctggtttcc accggcttcc tggaaggctt ttattacaag ccgcgcattg attatgacgt
     3481 tctggcaaaa caccacgagg gattgatcgc tctctcggcg tgcttgaagg gcgtggtgac
     3541 cgaagctgtc gtgacggaga aatacgacca ggcacgcgag aacgcttacc ggctacggga
     3601 tattttcggc aagcagaatt tctttttgga gattcaggac caagggctgg acatcgagaa
     3661 ggcggtgaat ccgcaactgg tgcggctatc gaaggaaacg ggaattccgc tggtagcaac
     3721 gaacgacttc cactacttgc atcatgaaga cgcgcacgcg caggaagtgc tgctgtgcat
     3781 ccagaccggc aagaccatgg gcgacgcgaa ccgcatgaag ttcgcgacgg atcaatttta
     3841 tttcaagagc gcggtggaga tggccaaggt cttcggggag attcccgaag tgctccggcg
     3901 cacggtggac atcgcttcgc gttgcaacgt aaaaatcgaa cggattccta atccctttcc
     3961 ggaattcaag gtgccggatg gccagactgc cagcagttat tttgaaaaag tagtacgcga
     4021 agggtttgcg acgcgagtgc cttacctgga gcggctggcc aaggccggcg cgctggccaa
     4081 tccgctgagc gaatacgagc gccggcttac cagcgaaatc cagatgattc agaaaatgcg
     4141 ctacgaaggg tattttctga tcgtttggga tttcattcac tacgcacggg cgcagggagt
     4201 gccggtgggg ccggggcgcg gatcggctgc aggtagtctg gtcagttatg cgctgcggat
     4261 taccgacgtg gacccgctgc aatacaacct gcttttcgaa cgattcctga atccggaacg
     4321 cgtgtccatg ccggatatcg atatcgattt ctgcatgcgg cggcgcggcg agttgattga
     4381 gtacgtcacg caaaaatatg gccgcgaaaa cgtggcgcaa atcatcacct tcggaaccat
     4441 ggcggcgaaa gcggccatca aagatgtggg ccgcgccatg gaaattccgt atgtggaagt
     4501 ggaccggctg gccaaaatgg tgccgactac cctgggaatt gagctgaagg atgcgctgga
     4561 agaagcgccg cagttgaagt cggcggtgaa cagcgacgaa aagctcaagg acttgatgaa
     4621 cgtggcgctg cggctggaag ggctttcacg ccacgcttcc acgcacgcgg ccggcgtggt
     4681 gatttcgccg cagccgttga cggaactggt tccgatctat aaaaccaacc gcgacgaaat
     4741 cacgacccag tacgacatga atgcgctgga gcgcatcggc ctcttgaaaa tggacttctt
     4801 ggggctgacg acgctcacgg ttcttcagga tgcggtgcgg atggttgagc agaaccgcgg
     4861 cgtcagagtg gatatcgaca atctgacgct ggacgatgcc gacacctaca agctcttttc
     4921 gcgtggcgat accaccgcta tctttcagtt tgaatcgcat gggatgcgcg acatcttgcg
     4981 tcgctaccag ccgacccgca tcgaggattt gaccgcgctg aacgctctct accgccccgg
     5041 accgattcag ggcggcatga tcgacgattt cattaatcgc aagcacggca aaacaaaagt
     5101 cagctacgaa ctgccgcaat tgcaggacat tctgcaggaa acctatggcg tcattctgta
     5161 tcaggaacag gtgatgcaga tcgctaaccg cttggcgagt ttttcgctgg gcgaagcgga
     5221 cattttgcgc cgcgccatgg gcaagaagaa aaaggaagag atggcggcgc agcgcgcgaa
     5281 attcatggcc ggctgcgcga agaacaagat caatgaaaag aaggccgaga aaatcttcaa
     5341 cctgatggaa gagttcgccg gctacgggtt caataagtcg cactcctgcg cttatgcgct
     5401 tttggcctat cagaccgcgt acatgaagac gcactatcct gtggaattca tcgccgcgct
     5461 gctgacttcg gaaaccggaa acgccgagaa agccgtaaag tacatcaacg aagcgcgcgg
     5521 gatgaacatt ccaattttgc cgccggacgt aaacgagagc gatttgtatt tcactccggt
     5581 aggcgagtcc atccgctttg ggctggcggc catcaagaac gtcggcgaaa acacggccaa
     5641 ggccatacgc gaatcgaagc tgagc
//
LOCUS       DASTPM010000570         3594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_135509, whole genome shotgun sequence.
ACCESSION   DASTPM010000570 DASTPM010000000
VERSION     DASTPM010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135509"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFR42_10100"
     CDS             <1..454
                     /locus_tag="VFR42_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0369553.1"
                     /translation="DLIFASVAVGLLIAAVQGALGGLAFTLAGVGTALFWGVLMAFFS
                     LVPVVGSALIWVPTALWLAFTGHIAKGLLVAVICGGVAGVTDNILRPLLLRNRTHLNE
                     LLLFISVLGGIQVFGLLGLVAGPTILAAAMGVFRVYMDHRDKVEAAQA"
     gene            513..2966
                     /locus_tag="VFR42_10105"
     CDS             513..2966
                     /locus_tag="VFR42_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0369554.1"
                     /translation="MNEPTTNGLKVTALPVDKLRWRCEPSRLPFETTAQAELREGLVG
                     QDRALRALKMGVELTAPGYNVFVCGLAGTSRGGTIAKMIEELHPQTKQSQDRCYVNNF
                     KFVDRPRLLTLARGQANTFKKDMQSGIDFLRRRIPQVFEGEPFQRQKGRIVERFTARE
                     KDLMDDFTRKIAREQFALGHMQVGAVALPEIFPVLEGQMVPIEDISKMVHEGKLDGPV
                     AEEIERKYEQFRQEFTVVYRKTLTLSRELASELSYLEQEAASVLVDGVIEELKEKYPG
                     DSVSEYLEEVRHHVLDNLDPFKEREGEDEDRPEGMEGVPKPTGPERDPFRVYGVNVIL
                     SHDGEAESPVIFETTPTYANLFGTIQRTYDARGGWSSDFMDLRGGSVLRADGGYLIMY
                     SLEAISEQGVWRALKRTLNHSRLEIQPVDMFYPFGVSAMKPEPIQISVKVILIGDREM
                     YELLYDYEEDFRKIFKVRVEFDEEMAMNDSVIAEYAGRLRALSEKEDLRPFDRGAFAA
                     MLEYGVRRAGRRNKVTARFVDIADIAREANYQANAAGESVVRAAHVRKALDSKIERHN
                     LIETRIREMIEEGTLLVDVSGERTGQVNGLSVLEIGGYSFGKPVRITASAALGKTGLI
                     NIEREANLSGRFHDKGVQIIAGFFRNRFAQDKPLSLAASICFEQSYSGVDGDSASSTE
                     VYALVSALAGLPLRQDLAVTGSMNQQGDIQAIGGVNEKIEGFFDVCRIKGLTGSQGVL
                     IPAANIEDLMLRDDVLDAVAAKQFHVWPVEKVEQGIEILTGMPAGHRNGDGSFEKQTV
                     FGLVDQRLADMAKKLKEFE"
     gene            complement(2986..>3594)
                     /locus_tag="VFR42_10110"
     CDS             complement(2986..>3594)
                     /locus_tag="VFR42_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0369555.1"
                     /translation="YTKGSESVIGFTTDKTEARLALRGINFAQGFGELNLFASVANAV
                     EGLGQYPGKKTIVLLSTGVDTSPDINWKALLPKLQIADVRIIAVSLAEGIRKPAKKKR
                     LTGEEKAKREQLAAGFAEGDQSLRNLSQATGGRVYFVQNEKDFAKAYAEIADLLRHEY
                     SVTFVPPMLDGKIHELRVEVKQPGVVVEHRPAYLAAMSGEVH"
BASE COUNT          745 a         1088 c         1001 g          760 t
ORIGIN      
        1 cgatctcatc ttcgccagcg tggcagtcgg gctgctcatc gccgccgtgc agggcgctct
       61 gggcggactg gcgttcacgc tggccggcgt cggcaccgca ttgttctggg gcgtgctgat
      121 ggcgttcttc tcgctggtgc cggtcgtcgg ttccgcgctg atctgggtgc cgaccgcgtt
      181 gtggctggcc tttaccgggc acattgccaa aggtttgctg gtcgctgtga tttgcggtgg
      241 cgtcgctggc gtcaccgaca atatcctccg gccgctgctg ctgcgcaatc gcacccattt
      301 gaacgaactc cttttattca tcagtgtgct cggcggcata caagtatttg ggttgctcgg
      361 cttggtggcc ggccccacca tcctggccgc cgcaatgggt gtctttcgcg tctacatgga
      421 tcatcgcgac aaagttgagg ctgctcaggc ctagaacttg gccgcctgcg cgccgccgcc
      481 gcgcagcccg gtgtgttaga gtaccgccga acatgaatga accgacgacc aacggtctaa
      541 aagtcacagc gctgccagtc gataaactgc gctggcgctg cgagccttcc cgcttgccgt
      601 ttgaaaccac ggcgcaggcc gaattgcgcg aaggattggt gggccaggac cgcgcgctgc
      661 gcgccctgaa gatgggcgtc gaactgaccg cgcccggcta caacgttttt gtctgcggac
      721 ttgccggcac cagccgtggc ggcaccatcg ccaaaatgat tgaagaattg catccgcaaa
      781 ccaagcagtc ccaggaccgc tgctacgtca ataatttcaa gtttgtcgat cgcccgcgcc
      841 tgctcaccct tgctcgcggc caggccaaca cgttcaaaaa ggacatgcaa tccggcatcg
      901 attttctgcg tcgccgcatt ccgcaagttt ttgaaggcga accattccag cgccaaaagg
      961 gaagaatcgt cgaacgcttc acggcgcgcg aaaaagattt gatggacgac ttcacccgca
     1021 aaatcgcgcg cgagcagttc gccctcggcc acatgcaggt cggagccgtc gcgctgccgg
     1081 aaatctttcc cgtgctcgaa ggccagatgg tgccgatcga agacatctcc aagatggtcc
     1141 acgaaggcaa gctggacggg ccggtcgccg aggaaatcga acgcaagtac gaacaattcc
     1201 gccaggaatt tacggtcgtc taccgaaaga cgctcacgct ttcgcgcgaa cttgccagcg
     1261 aactgagcta cctcgaacag gaagcagcct ccgttttggt ggatggcgtc atcgaggagt
     1321 taaaagaaaa atatcccggg gatagcgtct cggaatacct ggaagaagtt cgtcaccatg
     1381 tgctcgacaa cctggatcca ttcaaggagc gcgagggaga agacgaggat cgccctgaag
     1441 ggatggaagg cgtgcctaaa cccaccgggc cggaacgcga tccgttccgg gtttacggcg
     1501 tgaacgtcat tttgtcgcac gatggcgaag ccgaatcgcc ggtcatcttt gaaacgaccc
     1561 caacctacgc caatctcttc ggcaccattc agcgaactta tgacgcgcga ggtggatggt
     1621 ccagcgattt catggacctg cgcggcggtt cggtgctgcg cgccgacggc ggctacctga
     1681 ttatgtattc gctggaagcc atttccgagc aaggcgtttg gcgcgccttg aaacgcacgc
     1741 tcaaccacag ccgcctggaa attcaacccg tcgatatgtt ctaccctttc ggcgtcagcg
     1801 ccatgaagcc tgagcccatc cagatcagcg taaaggtgat cctgattggc gatcgcgaaa
     1861 tgtatgagct tctctacgac tacgaggaag actttcgcaa gattttcaaa gtacgggtgg
     1921 agttcgacga ggaaatggcc atgaacgatt cggtcatcgc cgaatacgcc ggccgtctgc
     1981 gcgcgctatc tgaaaaggaa gacttgcggc ccttcgaccg aggcgccttc gccgccatgc
     2041 tggaatacgg tgttcgccgc gccggtcgcc gtaacaaagt tactgcccgt ttcgtggata
     2101 ttgccgacat tgcgcgcgaa gcgaactatc aagccaacgc cgctggagaa tccgtcgttc
     2161 gggcggcgca cgttcgcaaa gcgctggact ccaagatcga gcgccacaat ttaatcgaaa
     2221 cgcgtattcg agaaatgatc gaggaaggca cgctgctggt ggacgtttcc ggcgagcgca
     2281 ccggccaggt caacggtctc agcgttctcg aaatcggcgg ctattccttc ggcaagcccg
     2341 tccgcatcac cgccagcgcc gcgctcggca aaaccgggct catcaatatc gaacgcgaag
     2401 ccaacctgag cggccgcttc cacgacaaag gcgtacaaat catcgccggg tttttccgca
     2461 atcgcttcgc gcaagacaaa ccgctctcct tggcggccag tatttgcttc gagcagtctt
     2521 attcgggcgt ggatggcgac agcgccagct ctactgaagt ttacgcgctg gtttctgctc
     2581 tggcgggctt gccgctacgg caggatctcg ccgttaccgg ctccatgaac caacagggcg
     2641 acatccaggc catcggcggc gtcaacgaaa agatcgaagg cttcttcgac gtctgccgca
     2701 tcaagggcct gactggttcg cagggtgttc tgattccagc tgcgaacata gaagacctca
     2761 tgctccgcga tgatgtgcta gatgcggttg ccgccaaaca atttcacgtt tggccagtgg
     2821 aaaaagtcga gcagggaatt gagattctca cgggtatgcc cgccggacat cgcaatggtg
     2881 acggttcttt tgaaaagcaa accgtcttcg gcctggtgga ccagcgcctc gcggatatgg
     2941 ccaagaaact caaagaattc gagtaacgcc gcgcgaagtc gcgcttcaat gcacttcgcc
     3001 gctcattgcc gctaaatacg ctggccgatg ctcgaccacc actcccggct gcttcacttc
     3061 cacgcgcaac tcatgaatct ttccgtccag catcggtggc acaaacgtca cgctgtactc
     3121 gtgccgcagc agatccgcga tctccgcata ggccttcgcg aaatcctttt cattctgtac
     3181 aaaatatact cggccgccag tagcttggct tagattgcgc agcgactggt cgccttccgc
     3241 gaaacccgct gccagttgtt cccgtttcgc tttctcctcc cccgtcagcc gtttcttctt
     3301 cgccggtttg cggatacctt ccgcaagcga cacggctata attcggacgt cggcgatctg
     3361 caatttcggc aacaaggcct tccaatttat gtcaggcgaa gtgtctactc cggtcgaaag
     3421 cagcacgatg gttttctttc cggggtattg acccagccct tccactgcgt tcgccacact
     3481 ggcaaacaaa ttcagttctc caaatccttg cgcaaagttg attccgcgca gcgccagcct
     3541 cgcctcggtt ttatccgtcg tgaaaccaat cacgctttcg ctccctttgg tata
//
LOCUS       DASTPM010000571         4113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_424050, whole genome shotgun sequence.
ACCESSION   DASTPM010000571 DASTPM010000000
VERSION     DASTPM010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424050"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..2300
                     /locus_tag="VFR42_10115"
     CDS             480..2300
                     /locus_tag="VFR42_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0369556.1"
                     /translation="MESQPANPVPRWKERLTALRNTLPIFRMVWEASPRIVAANVSVR
                     LIVALLPLAMLAVTKVIIDAIYQYTAHQAVLPSYFWWLVGAEFALACVSTILVRVGDY
                     CDTVLADKYAKHVSIRIMEHASRLDLTSYEDPTFQDKLERARVQGTDRVLMIQAAGRL
                     VQQLVTTISMALSVCFFSPWILVALVVCIVPAFLGETHFAFLGYSLSFAQTPARREME
                     YLRVLGGSKESAKELKLFGLGRYLVERFTNLAMRLHQETARLARRRVFVGSLLTLLGT
                     LGYYGTYAFVIDETVTGRLSMGTLILLSGAIAAASSNLQMVFSTFSTIADQAMFIGDL
                     LDFFSVRPAVLSKPNALPVPRPIKQGFEFRNVSFAYPNGRQVLHSVNFKLEPGERIAL
                     VGENGQGKTTIVKLLTRLYDPTGGQILLDGVDLREYDQEDLWREIGVIFQDFMRYDMT
                     ATENIAMGRIEERHNIFRIRSAAHKSLAEDVIRKLPKGFEQQLGCRFDGGTDLSGGEW
                     QKLALARAYLREAQVLILDEPTASLDAKSEHEVFERFAELTRGKMAMLISHRFSTVRM
                     ADRIFVLQDGRIAEEGPHDQLMQNGGRYAEMFELQAASYR"
     gene            2325..>4113
                     /locus_tag="VFR42_10120"
     CDS             2325..>4113
                     /locus_tag="VFR42_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369557.1"
                     /translation="MATGMELILNLRSAREWHSALEENNERNAVAAAAEETARRLEKI
                     LTAARRDGLKTQSKQRRKITSPMADNLALHEAQISGAIQYLQRRQASGAFLEGDWRTL
                     TANTKVIEEGFGESRAALRLIEELPSLIGAKEGQLRVRVVAERYLEAAEFVFERQELI
                     AFLAVLQEEAELTNAEIAALKGFLSQVLLEQVAGHARKLVMPGRNASASREEALPGLR
                     LALSSLKSLGLTDWDELFVETSLCEQALAQDPAGAFEGMDKAAKSDYRNAVAQLAREC
                     GESEAEVARRAVKLAKSPRVGTNARANERRSHVGFYLIGEGRQKLEGALGVHRSIFTR
                     LGRFLKRWPDGLYVVGIELLMIGLLSAVVMGAEIRVPTFLVVALFLLPAAESAVAIAN
                     QLAVMFVRPRSMPKMDYSKGIPAESKTLVVVPTLLVNREQMERAVRDLEIRFLGNRDA
                     NLHFALLTDPPDAPTQFDKRDEFGPECARLIEKLNQQYAGQRRGSFFLFHRNRTFNAV
                     EKIWMGWERKRGKLLDLNNLLLGKSNNFSVMAGDQKLLDGFKYVITLDADTQLARDSA
                     YRLVGAMAHPMQRAVIHPVSNVVVEGYGIL"
BASE COUNT          890 a         1074 c         1336 g          813 t
ORIGIN      
        1 gctgcgccag aaacagaatc ctcgagaaag gccggttggg aaagaccgcg cggaggcaga
       61 actgcaaggc gcaatgcgcg gcgaaagcag acgcgcgaat tgagcatgcc gagtgatcgc
      121 atcgggtggc gggacaacct aagagccacg agcgatccga gacttcgggt caagcgtctt
      181 aactgatacg gcaagagtta caaagactta gaagaaacga ccggctcgcc agagttggcc
      241 taagccacaa ctacaacaaa aatagagact tactgaaatc gcatcggtgg tactacgcat
      301 gcatatacag aggatagtgc ggagcgcgca gttttagtac taattggcgc actagcacca
      361 ggtcccatcg gttccgccgc tccgagcatg gcgatgcgag gtgagcgaga cagagataag
      421 gcatctctgt ccaagctgat ggtgtaaccg agcggcccca gccgtcgagg agagagtcag
      481 tggaatctca gcccgccaat ccggttcccc gatggaaaga gcgcctgacc gcgctgcgca
      541 acacgctgcc cattttcaga atggtgtggg aagcgtcacc gcggattgtg gcagcgaatg
      601 tcagcgtgcg gctgatcgta gcgcttttgc cgctggccat gctggcggtg acgaaagtca
      661 tcatcgacgc gatttatcag tacacggcgc atcaagccgt tttgccttct tatttttggt
      721 ggctcgttgg cgccgaattt gcgctggcat gcgtttccac gattctggtg cgcgtaggcg
      781 actattgtga cacggtgctg gccgacaagt atgccaagca cgtcagcatt cgcatcatgg
      841 agcatgcttc ccggctcgat ctgacctcct acgaagatcc cacctttcaa gacaagctgg
      901 aacgggcgcg cgtgcaaggc acggaccgcg tgctgatgat ccaggctgct ggccggttgg
      961 tgcagcagtt ggtaacgacg atcagcatgg cgttgagcgt ttgctttttt tcgccgtgga
     1021 ttctggtcgc cctggtggtt tgcatcgtgc cggcgtttct gggcgagacg cattttgcat
     1081 ttctcggtta ttcgctgagc ttcgcgcaaa ctcccgcacg ccgcgagatg gagtatctgc
     1141 gggtgctcgg aggaagcaaa gaaagcgcga aagagctgaa gctgttcggt ctcggacgct
     1201 acctggtgga gcggttcacc aacctggcga tgcgtctgca ccaggagaca gcgcggctgg
     1261 cgcggcggcg cgtgttcgtc ggctcgctgc tcaccctcct gggtacgctc ggctactacg
     1321 gaacgtatgc gttcgtgatt gatgaaacgg tgaccggtag gctgtcgatg ggaactctga
     1381 tcctgttgtc cggagcgatc gccgcagcca gttcgaactt gcagatggtg ttttcgacgt
     1441 tttcgacgat tgcggatcag gccatgttca tcggcgatct gctggacttt ttctccgtcc
     1501 ggccggccgt gctgtcgaaa ccaaatgcct tgccggtgcc gcggccgatt aaacagggat
     1561 ttgagtttcg caatgtttcg ttcgcgtacc caaacgggcg gcaggtgctg cacagcgtaa
     1621 atttcaagct ggagccgggc gagcgcattg cgctggtcgg cgagaacggt caaggcaaaa
     1681 cgaccatcgt gaagttgctg acgcggctgt acgacccgac gggcggacag attttgctgg
     1741 acggcgtgga cctgcgcgaa tacgaccagg aagatttgtg gcgggaaatc ggagtgatct
     1801 tccaggactt catgcgctac gacatgacgg cgacagaaaa catcgccatg ggacgcattg
     1861 aagaacgaca caatattttc aggattcgct cggcggcgca caagagcctg gcggaagacg
     1921 tgatccgcaa gctgccgaaa ggattcgagc agcagctggg ttgccggttc gacggcggca
     1981 cggatttgtc cggcggcgaa tggcaaaagc tggcgctggc gcgtgcctac ctgcgcgaag
     2041 cacaggtgtt gatcctggac gagccgacgg cttccctcga cgcgaagtcc gaacacgaag
     2101 tgtttgaacg gtttgcggaa ttgacgcgcg gaaagatggc catgctgatt tcgcaccggt
     2161 tttctacggt gcgtatggcc gaccggattt tcgtattgca ggacggcaga atcgccgaag
     2221 agggcccgca cgaccagttg atgcaaaatg gcgggcggta tgcagagatg ttcgaactgc
     2281 aagcggcgag ctaccggtag ctgggcgttg ggggaaagaa agcaatggcg acggggatgg
     2341 aattgatctt gaatttgcgc agcgcgcgag agtggcatag cgcgctggaa gagaacaacg
     2401 agcggaacgc tgtggctgcc gcggctgagg aaacagctcg ccggctcgaa aagatcctga
     2461 cggcggcgcg gcgcgatggt ctgaaaacac agagcaagca acgacggaaa atcacctcgc
     2521 cgatggccga caacttggcg ctgcacgaag cgcagatcag cggcgcgatc cagtacctgc
     2581 agcgtcggca ggcttccggc gcatttctgg aaggcgactg gcgaactctg acggccaaca
     2641 ccaaggtaat tgaagaggga ttcggcgaaa gccgtgccgc gttgcgtttg atcgaagaat
     2701 tgccgtcgct gattggggca aaagagggcc agctgcgtgt tcgtgtagtg gcggaacgtt
     2761 acctggaagc ggcggaattt gtttttgagc ggcaagagct gatcgcattc ctggccgtgc
     2821 tgcaggaaga agcggaactg accaacgcgg agatcgccgc gttgaaaggc ttcttgagcc
     2881 aagtgctgct cgaacaggta gccggccatg cgcgaaaact cgtaatgccg ggacgcaacg
     2941 ccagcgcgtc acgcgaagag gcactgcccg gacttcgttt ggcactttcc agcttgaaga
     3001 gcttggggct taccgattgg gacgaactgt ttgtggaaac cagcttgtgc gagcaagcgc
     3061 tggcgcagga tcctgccggc gcgtttgaag ggatggacaa ggccgcgaaa tccgattacc
     3121 ggaatgcggt ggcgcaattg gcacgcgaat gcggagagag cgaagcagaa gtagcgcggc
     3181 gagcggtcaa gttggcgaag tctccccgtg tgggaacgaa tgccagagcg aacgagcgtc
     3241 gctcgcatgt gggtttttat cttatcggcg agggacgcca aaaactggaa ggtgctctcg
     3301 gtgtgcaccg gtcgatcttt acccgactgg ggcgcttcct caagcgctgg ccagatggtc
     3361 tttatgtggt cgggatcgag ctgttgatga tcggcttgct cagcgccgtg gtaatgggcg
     3421 cggaaattcg cgtgccgacg tttctggtcg tagcgctgtt tctgctgccg gcggcggaaa
     3481 gcgcagtggc cattgcgaat caattggcgg tgatgtttgt gcggccgcgc tccatgccga
     3541 aaatggacta ttcaaagggc attccggcgg aaagcaagac cctggtggtg gtaccgacgc
     3601 tgctggtaaa ccgcgagcag atggaacgtg cggttcgcga tctggagatc cgttttctcg
     3661 gcaaccgtga cgcgaacctg cacttcgctt tactgaccga tccgcccgat gcgcccacac
     3721 aattcgacaa gcgcgacgaa ttcggcccag aatgcgcgcg gctgatcgag aagctgaacc
     3781 agcaatacgc gggacaaagg cgcggcagct ttttcctgtt ccatcgcaat cgcacattca
     3841 atgccgtgga aaaaatctgg atgggctggg agcgcaagcg cggaaagctc ctcgatctga
     3901 acaacctgct gctcggaaaa tctaataact tctcggtgat ggcgggcgat cagaagctgc
     3961 tagacggctt taaatacgtc atcactttgg acgccgatac ccaactggcg agagactccg
     4021 cctaccggct ggtgggcgcg atggcgcatc cgatgcagcg cgcggtgatt cacccggttt
     4081 ccaacgtggt ggtcgagggc tacggaatcc tgc
//
LOCUS       DASTPM010000572         4514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_623570, whole genome shotgun sequence.
ACCESSION   DASTPM010000572 DASTPM010000000
VERSION     DASTPM010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623570"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..676)
                     /gene="msrB"
                     /locus_tag="VFR42_10125"
     CDS             complement(89..676)
                     /gene="msrB"
                     /locus_tag="VFR42_10125"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU0369558.1"
                     /translation="MNDLTRRGLIFSAAGLVSLAVFAGLDRVASSSLLEKEEPEDPNA
                     TVEIVQFDESGKRTGKVRLQKIHKTIEQWRRSLTPSQFAITRRGGTEIPYAGELVNEH
                     RPGVFRCADCGTALFDCKAKFESGTGWPSFWEAIAPENIHERDDFSMGILRREVKCTL
                     CDAHLGHVFTDGPDPTGLRYCMNSAAMKFSPLRAA"
     gene            complement(746..2575)
                     /locus_tag="VFR42_10130"
     CDS             complement(746..2575)
                     /locus_tag="VFR42_10130"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0369559.1"
                     /translation="MNFTLRLAVLLLAAACGLTSSSFAQAPADNDHTLQAMRDEMARA
                     KSRLELKFPGTNEPVRPYYIEYRLLDLDVREVVAQFGALMNSAHTRNRFMNVEARVGS
                     YKQDSSNFISDEGFRGFIGSTGSVGIDRDYDSLRQDLWIATDQAFKEAVETYSRKRAY
                     LTSLARQSDIDDFSQVAPVKKIEPLVTADWSNRNWEQEARESSAALRAFPDVQESRVT
                     YYLVYATEYLLTSEGTEIRTNRTFAAVEAGMNTLAKDGMELNNFYASYAPKPAELAGV
                     DTVKKALNVKGSELMAMRASPTALDYTGPVLFDSRAAAPLVAQVLGPALNGARPPVAF
                     QPVMEQFLSNIGGRSDWVNRTGSRVLPNGVTIVDDPSAKEFQGKPLIGGYSVDDEGVN
                     AEKVTAVDGGILKELLMSRRPGSDSLKSNGHGRSAFLNDAKPTMSNLFFNASETVSSA
                     DLKKKFLDICRANKQEYCLEIKEMDNPAISLLHQDDFSELLASYGGGAGSGDRLPLLV
                     YRVYPADGHEEIIRGARITGLNSRVLRNVTAVGSDPFVYNYMQTQVAGFAGTALGAFG
                     SAQNGLPASVVAPSLLFEELDVRGARGEPKRPPLVAAPEMTAK"
     gene            complement(2572..4335)
                     /locus_tag="VFR42_10135"
     CDS             complement(2572..4335)
                     /locus_tag="VFR42_10135"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0369560.1"
                     /translation="MRKSKRILAGAIVPALIFAMGAGLRPTAAHAGPPDTPPPVGKPS
                     PLLGAMQSELDRSFKTYSAQDPVAYFIGYTITDTQRAEVSGSNGALLSSAENHNRWLQ
                     VSVRAGSYTLDNTHKVGEQRMPSGGPGTGVPIDNDEQIVRRAIWLETDSQYRNAAEAL
                     IKVRSSKEVKADTAEGRAPDFSHEQPHKSIEPEVSFTLDRKPWEAKVRAYTKAFRESP
                     DILNSIVTFSALAQNSYLVNTEGTQLQSGQIRYRLELFIQGKATDGMNIERYYNFDWV
                     NPNDAPSDKDVQAASATLRKEMEGLVKAPLNEPSVGPALLTGRAAAVFFHEVFGHRAE
                     GHRQKDISEGQTFAKKVGEPILPDFLSIVDDPTLRKLSNEDLLGYYRFDDEGVSSHEV
                     ILVDHGVLKNFEMSRSPLADFPHSNGHGRRQLGATPVSRQGNLIVKSSKAVTNAQLRA
                     QLIELLKKQNKPYGLLIDDIAGGFTFTGRGQPQAFQVQPLVVYKVFADGKPDELVRGV
                     DIVGTPLAALTKIVATGDTPEVFNGYCGAESGSVPVSAASPAILTSELEVQKKQTDTD
                     APPILPPPAHDTKAAQTGGAR"
BASE COUNT          862 a         1389 c         1287 g          976 t
ORIGIN      
        1 ttttctctgg tttcgcttaa aggcagggca tccatggccg cgtctagact accttaagtg
       61 gtgcgagaga caagaaaagc agaaggctct atgcagcgcg caatggagag aacttcatcg
      121 cggcggagtt catgcaataa cgcagacccg tgggatctgg gccatcggtg aagacgtggc
      181 ccaggtgggc gtcgcagagc gtgcatttca cctcacggcg cagaatgccc atgctgaaat
      241 cgtctctttc gtggatattt tccggtgcga tagcctccca aaagctgggc cagccggtgc
      301 cggattcgaa tttcgctttg cagtcgaaaa gggcggtgcc gcaatccgcg cagcgaaaaa
      361 ctccaggccg atgttcattc acaagttcac ccgcataggg aatctcggtg ccgcctcgcc
      421 gcgtgatggc gaactgcgaa ggcgtcaaac tgcggcgcca ttgctcgatt gttttgtgaa
      481 ttttctgaag gcgaacttta cctgtccgtt tgccactctc gtcgaactgc acgatttcga
      541 ccgtagcgtt cggatcttca ggctcttcct tttcgagcag actggaactg gcgacacgat
      601 ccaagcccgc gaacacggcg aggctcacca gtccggcagc gctgaaaatc aatccgcgtc
      661 gagtcaggtc gttcatcgca gaatttgtag cagccgcttt ctgaagcggg cgaattccct
      721 agccgggccc ggcgcaaaca tctcgctact tggcagtcat ctccggcgcg gccaccagcg
      781 gaggccgctt cggctcgccg cgcgccccgc gcacgtccaa ttcttcaaag agcagtgacg
      841 gcgcaaccac ggaagccggc aggccgttct gcgccgaacc aaaagcgccg agcgcggtgc
      901 ccgcaaatcc cgctacctgt gtctgcatat agttgtacac aaacggatcg ctgccgaccg
      961 cggtgacgtt gcgcagcacg cgcgaattta gtccggtaat gcgcgcgccg cgaatgattt
     1021 cttcgtggcc atccgcggga tacacgcgat agaccagcaa tggcaaacgg tcgccgctgc
     1081 ccgcgccgcc gccgtagctg gccagcagtt cgctgaaatc atcctgatgc agcaggctga
     1141 tcgcgggatt gtccatttcc ttgatttcca ggcaatactc ctgcttgttg gcacggcaaa
     1201 tatcgaggaa cttcttcttc aagtccgctg acgaaacggt ttcgctggca ttgaaaaaca
     1261 gattgctcat ggtcggcttg gcgtcgttca agaatgcgga acgcccgtgg ccattggact
     1321 tcaaggaatc cgacccgggg cgccgcgaca tcagcagttc tttcaggata ccgccatcca
     1381 ccgcggtcac tttttccgcg ttgacgcctt cgtcgtccac gctgtagccg ccgatcagcg
     1441 gcttgccttg aaattccttt gcagacgggt cgtctacaat cgtcacgccg tttggcagga
     1501 cgcgcgagcc ggtgcgattc acccaatcgc tgcgaccgcc aatgttgctt aggaactgtt
     1561 ccatcaccgg ctgaaacgcc accggcggac gagcgccatt cagcgccggc ccaagcactt
     1621 gcgccaccag cggagccgcc gctcgcgaat cgaaaagcac cggcccggtg tagtcgagcg
     1681 cggtgggcga ggcgcgcatg gccatcagct cgctgccctt cacgttgagc gcttttttca
     1741 cggtgtccac gccggcgagt tccgcgggtt ttggagcgta cgacgcgtaa aaattgttca
     1801 gctccatgcc gtccttggcc agcgtgttca tgccggcttc caccgcggca aacgtgcgat
     1861 tggtgcgaat ctccgtgcct tcgctggtca gcagatattc cgtcgcgtaa accaggtaat
     1921 aggtcacgcg cgattcctga acgtccggaa acgcccgcaa cgccgccgaa ctttcccgtg
     1981 cttcctgttc ccagttgcgg ttgctccagt ccgctgtgac cagcggttca atttttttca
     2041 cgggcgcgac ctgcgaaaaa tcatcgatgt ccgattgccg cgccagactg gtcaaatacg
     2101 cgcgcttgcg cgaataggtt tccaccgctt ccttgaaggc ctggtcggtg gcaatccaca
     2161 gatcctgccg cagcgaatca tagtcgcggt cgatgcccac tgatcctgtg gagccgatga
     2221 agccgcggaa tccttcgtcg ctgatgaagt tggagctgtc ctgtttgtag ctgccaaccc
     2281 gcgcttccac gttcatgaaa cggttgcgcg tgtgcgcgct gttcatcagc gccccgaatt
     2341 gcgccaccac ttcgcgcaca tccaggtcca gcaagcgata ttcgatgtag taggggcgta
     2401 ccggctcatt cgtgccggga aatttcagct ccaagcgact cttggcgcgg gccatttcgt
     2461 cgcgcatcgc ctgcagcgtg tggtcgttgt cggcgggcgc ctgcgcaaac gaactcgacg
     2521 tgaggccgca tgcagcagca agaagtaaaa ctgcaaggcg cagcgtaaaa ttcatcgcgc
     2581 acctccagtc tgcgccgctt tcgtgtcgtg cgcaggcggc ggcaaaatcg gcggagcgtc
     2641 cgtgtcggtc tgttttttct gcacttccag ctccgaagtc aaaattgccg gcgaagctgc
     2701 ggacacgggc acggaacccg attccgctcc gcagtatccg ttgaagactt ccggcgtatc
     2761 gcccgtggcg acaatcttgg tcaacgccgc cagcggagtg ccgacaatgt ccacgccacg
     2821 cacgagctca tcgggcttac cgtcggcaaa gactttataa acgaccagtg gttggacctg
     2881 aaacgcctga ggctgaccgc ggccggtaaa tgtaaatccg cctgcaatgt cgtcgatcag
     2941 caatccgtat ggcttgttct gcttcttgag cagctcgatc aactgcgcgc gaagctgcgc
     3001 attcgtcacg gctttgctgc tcttcacgat caggttgccc tgacgcgata ccggcgttgc
     3061 gcccagctgc cgccgtccgt gcccgttcga atgcggaaaa tccgccagcg gtgagcgcga
     3121 catctcaaaa ttcttcaaca cgccgtgatc caccaggatt acttcgtggg aggaaacgcc
     3181 ttcgtcatcg aagcggtaat aacccaacaa gtcttcgtta ctcagtttcc taagcgttgg
     3241 atcatccacg atgctcagga agtccggcaa gatcggctcg cccacttttt tcgcgaaggt
     3301 ctgcccctcg gaaatatcct tttgccggtg gccctctgcg cggtggccaa agacttcatg
     3361 gaaaaagacg gcggccgcgc gccccgtgag caacgctggc ccgacggagg gttcgttcag
     3421 cggagccttc accagccctt ccatttcttt tcgcaaagta gcgctcgcgg cctgcacatc
     3481 cttgtcgcta ggcgcgtcgt ttggattcac ccagtcaaag ttgtagtagc gctcgatgtt
     3541 cattccatcg gtcgctttgc cctggataaa cagttccagc cggtagcgaa tctgcccgct
     3601 ctgcagttgc gtcccttcgg tgttcaccag gtaggaattc tgcgccagcg ccgaaaacgt
     3661 cacgatggag ttcagaatgt ccggtgactc acgaaacgct ttggtgtatg cgcgaacttt
     3721 cgcttcccac ggcttgcggt ccagtgtgaa cgacacttcg ggctcgatgg atttgtgcgg
     3781 ctgctcgtgc gagaaatccg gcgcgcggcc ctccgccgtg tccgccttaa cttctttgct
     3841 ggagcgaacc ttgatcagag cctcggccgc attgcggtat tgcgaatccg tttccagcca
     3901 gatggcgcgc cgcacaatct gttcgtcgtt gtctatggga acgccagtgc cgggcccgcc
     3961 gcttggcatg cgctgttcgc ccactttgtg cgtgttgtcg agcgtgtagc tgcccgcgcg
     4021 cacggaaacc tgcagccaac ggttgtggtt ctccgcgctc gatagcagcg ccccgttcga
     4081 acccgatact tccgcgcgct gtgtgtcggt aatcgtgtag ccgatgaaat acgccacggg
     4141 atcctgcgcg ctgtacgtct tgaaggagcg atccagctcc gactgcattg cgccgagcaa
     4201 tggggaaggt tttcccaccg gcggcggcgt gtccggcggg ccggcatgtg ctgccgtggg
     4261 tcgcagccca gctcccatgg caaaaatcaa cgccggaacg atggctccgg ccaaaatacg
     4321 cttcgattta cgcaaagaag tcctcacggt ccaaaacgga tgcaattggc taaaatcaca
     4381 gtgtacgtca ctctttggcg ggcttacaac gcggcgcctt gccggcccta cgtcaattcg
     4441 acggcttttc gctgcgtttc gttgcacgtc cctgcggctc ttttgagagc gaagggccgg
     4501 ctgaattcca tcga
//
LOCUS       DASTPM010000573         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_798940, whole genome shotgun sequence.
ACCESSION   DASTPM010000573 DASTPM010000000
VERSION     DASTPM010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798940"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="VFR42_10140"
     CDS             <1..1216
                     /locus_tag="VFR42_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU0369561.1"
                     /translation="IPLEKVSTSMTINATAAILLCLYVAVAKKQGASLAKLSGTVQND
                     ILKEYIARGTYIYPVRPAMRIVTDLFAWCKDELPRWNTISISGYHIREAGSTAVQEVA
                     FTLADGIAYAHSALDAGLQVDEFAPQLSFFFNVHNDFLEEIAKFRAARRLWARIMKER
                     FGARDERSLMLRFHAQTAGSSLTAQQAENNIVRVGLQALAAVLGGCQSLHTNSLDEAL
                     ALPTEDSALIALRTQRIIAHETGVANTIDPVAGSYAIEALTAQIEKAATVYLEQIDAL
                     GGMLAAIESGYVQKEIHKSAFEYQRAVETKEQIVVGVNELQAEQERQIPTLRIDPAME
                     REQVARLEALRNKRNAAKTQEAMAELGRRAKGSENLLPAILSAVENFATVGEISDTLR
                     RFFGEYQESVVL"
     gene            1295..1870
                     /locus_tag="VFR42_10145"
     CDS             1295..1870
                     /locus_tag="VFR42_10145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEU0369562.1"
                     /translation="MKLILASASPRRAEILRAAGIAFTLLSSAVDETPLPGETATDMV
                     RRLAAAKAELVAARAVGPAIVIAADTVVALDGAVMGKPRTSDDARHMLEKLSGRTHSV
                     ITGVALVRLPDVERREFTEITQVHFTALSDEEIVRYLSSGEPFDKAGAYAIQGVAGRF
                     IPRIDGCYFNVVGLPLARLCRELTALGWFGE"
     gene            complement(1953..2156)
                     /gene="tatA"
                     /locus_tag="VFR42_10150"
     CDS             complement(1953..2156)
                     /gene="tatA"
                     /locus_tag="VFR42_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU0369563.1"
                     /translation="MEFPMHWLIILVVVLILFGGRKIPELMRGLGEGVRGFKEGMSGN
                     GPKGTPPAQSASTDKPAEDKPQP"
     gene            complement(2230..>3950)
                     /gene="selB"
                     /locus_tag="VFR42_10155"
     CDS             complement(2230..>3950)
                     /gene="selB"
                     /locus_tag="VFR42_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU0369564.1"
                     /translation="PGHERFVANMLAGAAGVDLVLLVIAADESIKPQTRERFDICRLL
                     GIQSGLVALTKSDLVDRDTLELARLEIEDYLRGSLLEGVPILAVSTKTGAGLPELKRA
                     LVAQAQQLPGKDSQRDFRLPIDRSFSIKGFGAVVTGTLLDGRVSPSDEVELFPAGTRL
                     RVRGVQSEGKAAERGVAGQRIAINLAGVDHRALKRGMTLAAPGRYRATRRVDVKLQLL
                     PSVRRLKNRAQVHFHSGSFEAMAEVALFGRTELNGGGEAFAQFKLKQSALLLPGDRFI
                     IRQSSPLTTIGGGTILDPIPRRPRANEAPREVFLAAVERADKAEILARLVERSLFGLP
                     QREISARTGWSDAEIRATIRGLAAEARVRVVAADSPIFYSAAAFGELLKKFSERVELF
                     HKQNPLQPGIAREDLRVSLTRRLRPEIFRAALDELAAQKKIELQGELVRRAGAQISLL
                     PEEAKAKEQIEAAFASAGLAVPSAKEVLGKLSIEARRSEKLLQLLLRDKALLRITPEL
                     IFHRDALAELRSKLVSYKKAKGERISVPAFKDLTGITRKYAIPLLEYLDRERVTRRQG
                     DERVIL"
BASE COUNT          786 a         1134 c         1222 g          808 t
ORIGIN      
        1 aataccgctg gaaaaagttt cgacgtcgat gacgatcaat gctactgctg cgatcttgtt
       61 gtgcctctac gtggcggtgg ccaagaagca gggcgcgtcg ctggcgaagc tttcgggcac
      121 ggtgcagaac gatattttga aggagtacat cgcgcgcggg acgtacatct atccggtgcg
      181 gccggcgatg cgcatcgtga cggatttgtt cgcgtggtgc aaagacgagc tgccgcgctg
      241 gaataccatt tcgatttccg ggtaccacat tcgcgaagcg ggatcgacgg cggtgcagga
      301 agtggcgttc actttggcgg acgggattgc ttatgcgcat tcggcgctgg atgcgggctt
      361 gcaggtggat gagttcgcgc cgcaattgtc gttctttttc aacgtacata atgattttct
      421 tgaggagatt gcgaagtttc gcgcagcgcg gcggctgtgg gcgcgcatta tgaaggagcg
      481 gttcggagcg agggacgagc ggtcactgat gttacggttt cacgcgcaga cggcgggatc
      541 gtcgctgacc gcgcagcaag cggagaacaa tattgtgcgc gtcggactac aggccctggc
      601 cgcggtgctc ggcggctgcc agtcgctgca cacgaattcg ctggatgagg cactggcgct
      661 gccgacggaa gattcggcgc tgattgcttt gcggacgcag cggatcatcg cgcacgaaac
      721 gggcgtggct aatacgattg atccggtggc gggatcctac gcgatcgagg cactgactgc
      781 acaaattgag aaagctgcaa ccgtgtattt ggagcagatt gacgcgctgg gaggaatgct
      841 ggcggcgatc gagagcggct acgtccagaa agagattcac aagtcggcgt tcgagtacca
      901 gcgggcggtg gaaacgaaag agcaaatcgt cgtcggtgtg aatgaattgc aggcggaaca
      961 agaacgtcag atcccgacgc tgcgcattga cccggctatg gaacgcgaac aggtggcgcg
     1021 tttagaggcg ctgcgcaaca agcggaatgc ggctaaaacg caggaggcga tggcggagct
     1081 tggccggcgc gccaagggca gcgagaatct tcttccagcg atcctcagcg cggtcgaaaa
     1141 ctttgcaacc gtcggggaga tttccgatac ccttcgccgg ttctttggcg agtatcagga
     1201 atcggtcgtt ctctagactg aaccggctcg tttctggccc aaccaacagc tgctgcagca
     1261 aggcggctgt agtattcttc cggcagcgtt cgccatgaag ttgattcttg cttccgcttc
     1321 gccgcgccgc gcggaaattc tgcgcgctgc cggcatcgct ttcacccttc tttcttcggc
     1381 agttgacgaa actccgcttc ccggagaaac cgcgacagat atggtgcggc gcctggctgc
     1441 cgccaaagcg gaactggttg ccgcacgcgc tgttggcccg gccattgtga ttgcagcgga
     1501 caccgtggtc gccctcgatg gcgccgtaat gggcaaaccg cgcacctccg acgacgcgcg
     1561 gcatatgctc gagaagcttt ccggccgcac gcattccgtc attaccgggg tcgccctggt
     1621 tcgtctgccg gatgtggagc gccgtgaatt caccgaaatc acccaggtgc atttcaccgc
     1681 gctgtccgat gaggaaatcg tgaggtatct ttcctccggc gagcctttcg acaaagccgg
     1741 agcttatgcg atccagggag ttgccggtcg cttcattccg cgcattgacg gttgttactt
     1801 caacgtcgtt ggactgccgc tggcccgcct gtgccgcgaa ctgactgcgc tcggctggtt
     1861 cggcgaatag gaagccatcg cgctgaaaac cagctgaaaa caaaagggcg acaatttcgt
     1921 cgccctttat aaactcaagt tttttggagc gttcagggct gaggcttgtc ttcggcaggc
     1981 ttatccgtgg acgccgactg cgccggtggc gtgcccttgg gaccgtttcc cgacatgcct
     2041 tccttaaatc cgcgcacgcc ttcgcccaat ccgcgcatca gctcgggaat cttgcggccg
     2101 ccaaaaagga tcagcacgac gaccagaatg atcagccagt gcatagggaa ttccatactg
     2161 cctcctgccc ttactaaccc gaatctagca cgttgccaac gaaggtcaac gagcgaaaag
     2221 ggcgcgaaat cagagtataa cacgctcgtc gccctgccgc cgggtcacgc gttcgcggtc
     2281 cagatactcg agcagcggga tggcatattt gcgcgtgatg ccggtcaggt ctttaaaagc
     2341 tggcacggag attcgctccc ctttcgcctt tttgtaggac accagcttgc tgcgcagttc
     2401 ggccagtgcg tcgcggtgaa aaatcagttc cggggtgatg cggagcaacg ccttatcgcg
     2461 tagcagaagt tgcagcagct tttccgatcg ccgcgcctca atggagagct tacccagaac
     2521 ctctttggct gatggaaccg ccagaccagc agaagcaaac gcagcttcga tctgctcttt
     2581 ggctttggct tcttccggca gaagtgaaat ctgagcgcca gcccttctga ctagctcgcc
     2641 ttgcagttcg atcttttttt gcgcggccag ctcgtccagc gccgcgcgaa agatttcggg
     2701 ccgcaggcgg cgggtgagcg aaacgcgcaa atcttctcga gcgatacctg gctgcaaagg
     2761 gttttgtttg tgaaagagct cgacgcgctc cgaaaatttc ttgagcaatt cgccgaacgc
     2821 ggcggctgaa taaaagatgg ggctatccgc cgcgacgacg cgaacgcgcg cctcagctgc
     2881 caggccgcga atcgtcgctc gaatctccgc gtcggaccag ccggttcgcg cggaaatctc
     2941 tctttgcggc agaccgaaca gtgagcgctc gaccagccgg gcgagtattt cggccttgtc
     3001 ggcgcgctcc accgcggcga ggaaaacttc gcgtggcgcc tcgttggcgc gcggacgccg
     3061 cggaatggga tccaggatgg ttccgccgcc gattgtggtg agtggcgaag actgccgaat
     3121 gatgaagcgg tcgccgggaa gcagcagcgc gctttgtttc aacttgaact gtgcgaaagc
     3181 ttcaccaccg ccgttgagct ccgttctgcc gaacagcgct acttccgcca tcgcttcaaa
     3241 ggagccgcta tgaaagtgga cttgtgcgcg attcttcagt cttcgcacgg atggcaggag
     3301 ctgcagtttc acatctacgc ggcgcgtggc gcgataacgg ccgggagccg ctaaggtcat
     3361 accgcgtttg agcgcgcgat gatcgacacc cgcgaggttt atggcgatgc gctgccctgc
     3421 cacgccgcgt tcagcggcct tgccttccga ttgcacgcca cggacgcgca ggcgcgtacc
     3481 cgcgggaaac aattccactt cgtcgctcgg cgatacgcgc ccgtcgagaa gagttccggt
     3541 cacgacggcg ccaaaaccct tgatggaaaa tgaccgatca atgggcaggc gaaaatcgcg
     3601 ttgcgagtcc tttcccggca attgctgcgc ttgcgcaacc aaagctcgct tcaattcggg
     3661 caagcccgct ccggtttttg tgcttacggc aaggatggga acgccttcca gaagcgagcc
     3721 gcgcaggtag tcttcgattt cgagccgcgc aagttcgagc gtgtcgcggt ccaccagatc
     3781 ggacttggtc agagcgacca gaccgctctg aattcccagc aacctgcaaa tatcaaaacg
     3841 ttcgcgcgtt tgcggcttga tggactcatc ggcggcgatt acgagcagga ccagatcaac
     3901 gcccgctgcg cccgccagca tgttggccac gaaacgttcg tggccgggaa
//
LOCUS       DASTPM010000574         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_827549, whole genome shotgun sequence.
ACCESSION   DASTPM010000574 DASTPM010000000
VERSION     DASTPM010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827549"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFR42_10160"
     CDS             complement(<1..187)
                     /locus_tag="VFR42_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369565.1"
                     /translation="MTGFMDLKKLWNRRTFLGSAGMFVGMLFNARKAFGLTNRAARLG
                     AVALPEKLTGLGSTGNVY"
     gene            complement(184..699)
                     /locus_tag="VFR42_10165"
     CDS             complement(184..699)
                     /locus_tag="VFR42_10165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0369566.1"
                     /translation="MFSSFTSRRGFTARLAAIIPGLAATGAALRAAPGSPESQAGSGV
                     KKLDYEGKLAGTGFITPLILHNDTIYIAGQGAHSHDPKGEFPMDIETHTKKVMDNVKT
                     LVETGGGTMDSILQLTVFLTKIDDYDGMNKIFKTYFPNGGPARTTVAVAALPGNSLVE
                     INCIAAIVKKG"
     gene            complement(751..2349)
                     /locus_tag="VFR42_10170"
     CDS             complement(751..2349)
                     /locus_tag="VFR42_10170"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEU0369567.1"
                     /translation="MIGAAVLCAAQEPGPGLVSTSIDVRNEHLLVRPVAADWPSYNGD
                     YTGRRYSALRQITPANVAQLTAQWVFHAPNSSYLEVTPVVVGGIMFLTAANDAYALDA
                     QTGRTIWHYSRPVTEGLIDDAAQHHNRGVAILGHRVFMETDNAHLLCLDARSGNVLWD
                     VAYTDGNKNYGATSAPLVVKNKVIVGTSGGDDGVRGFVTAYNAETGKEAWRFWTIPGP
                     GEFGSQSWPGESYKLGGGTTWMPGTYDPELNTIFWGTSNPAPDFDGAPRPGDDLYTDC
                     LLALDPDTGKLKWYFQFTPHDLFDYDAVETPVLVDATFRGEPRKLIVQANRNGFLYVL
                     DRTNGAFLSATRFVEKLNWASGIDAKGRPILTGVRPNEAGARICPGFSGATNWYSPSY
                     HPETGMFYFLALENCHVYIRKEKEFKAGQTYYSTGIRHSAGDFGEKILLAFTLESDKP
                     VWRYVQGGEGRSSAGVMTTAGGLLFFGDDAQSFEGVDAKTGQALWHFNTGQNISASPM
                     SYAVNAKQYVAIAAGSNVFSFALP"
     gene            complement(2508..3311)
                     /locus_tag="VFR42_10175"
     CDS             complement(2508..3311)
                     /locus_tag="VFR42_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU0369568.1"
                     /translation="MRIQFQLKAFLSRRIRRPGHAAFLGAAALTLGLFFASARGGATQ
                     GAAAQSPSSDAALLDGFRHVEVASISDALEQLTGKKMYMTHRMRPIFPTRFAGHALTV
                     LLKKETNHDPDALKGMLHAIDTGGPNSVYVMVVEDGADIAGMGGLMGTAMSARNFSGA
                     VIDGGVRDTAYLQKIGFPVYALGAVPSTSVGHYRFAGANIAVTCDGVAVNPNDFIVAD
                     GDGVVVVPAGVAAQVLQLAQEMDFKEHSMYAHIERLKSIEEAVAKFGRL"
BASE COUNT          747 a         1096 c          921 g          758 t
ORIGIN      
        1 catagacatt ccctgtcgag ccgaggccgg tcagtttctc cggcaacgca acagcaccta
       61 accgagccgc ccggttcgtc aggccaaaag ctttgcgcgc gttgaacagc attccgacaa
      121 acatcccggc actgccgaga aatgttcgcc ggttccaaag ctttttcagg tccataaatc
      181 cggtcatccc ttctttacga tcgcagcgat acagttgatt tccaccagcg aatttccagg
      241 caacgccgcg accgcgaccg tggtgcgcgc cggccctcca ttcggaaaat acgtcttgaa
      301 gattttgttc atgccgtcgt agtcgtcaat cttcgtaaga aaaacggtga gctgcagaat
      361 gctatccatc gtcccgccgc cggtttccac caaggtcttg acgttgtcca tcactttttt
      421 cgtatgagtc tcgatatcca tgggaaattc gcccttggga tcatgcgagt gcgccccttg
      481 tcccgcaata taaatcgtgt cgttgtgcag gatgaggggc gtgataaaac cggtgccggc
      541 cagcttgcct tcgtaatcga gtttttttac tccagaaccc gcttgtgact ctggcgaacc
      601 aggtgccgcg cgcagcgccg ctccggttgc ggcgagcccc ggaatgatcg ccgcgagccg
      661 agccgtgaac ccgcgccgtg aagtgaaaga tgaaaacatg ttcttctcct tgtacgaaag
      721 ttgaccgaaa aggtcttaca aactgcgaaa tcagggcaat gcaaaactga aaacgttgct
      781 ccccgccgca atcgccacat actgcttcgc gttcaccgca taactcatcg gagaagccga
      841 aatgttttgc ccggtgttaa agtgccacag ggcttggccg gtttttgcat ctactccttc
      901 aaacgattgc gcgtcgtcgc cgaagaaaag cagcccgccc gccgtcgtca tcacacccgc
      961 ggaagaccgc ccctcgcccc cctgcacata gcgccaaacc ggtttgtcgc tttccagagt
     1021 gaaggccaga agaatttttt ctccgaagtc gcctgcgctg tgccggattc cagtggagta
     1081 atacgtctgc ccggccttga attccttctc tttgcgtatg tacacgtggc aattttccag
     1141 tgctaagaag taaaacatcc cggtttccgg atggtacgac ggcgaatacc aattcgttgc
     1201 gccggaaaat ccagggcaga tgcgcgcacc cgcttcgttg ggccgcactc cggtgagaat
     1261 cggccgcccc tttgcgtcaa ttccgcttgc ccaattcaat ttttcgacaa accgcgtagc
     1321 cgacagaaac gcgccgttcg tgcgatccag cacgtaaaga aaaccgttgc ggtttgcctg
     1381 aacaatcagt ttgcggggct cgccgcgaaa cgtcgcgtcc accagcactg gcgtctccac
     1441 cgcgtcgtag tcgaacagat cgtgcggcgt gaactgaaaa taccatttaa gtttgccggt
     1501 gtcgggatcg agcgccagca ggcaatcggt gtacaaatcg tcgcccggtc gcggcgcgcc
     1561 atcaaagtcc ggcgccgggt tgctggttcc ccaaaaaata gtgttgagtt caggatcgta
     1621 cgtgccgggc atccacgtag taccgccccc gagtttgtaa ctttcgcccg gccagctctg
     1681 cgaaccgaat tcgcccggtc ccggtatcgt ccagaagcgc catgcctctt ttcccgtttc
     1741 cgcgttgtac gccgtcacaa aaccgcgcac cccgtcgtct ccgcccgatg ttccaacaat
     1801 caccttgttc ttgacgacca acggcgcgct ggtggccccg taatttttgt tcccatcggt
     1861 gtacgcgaca tcccatagca cgttgcctga ccgcgcatcc aggcagagca ggtgcgcatt
     1921 gtccgtttcc atgaacacgc gatggcccag gatcgccact ccgcggttat gatgctgcgc
     1981 cgcgtcatcg atcaaacctt cggtcaccgg ccgtgaataa tgccagatcg tgcggccagt
     2041 ttgcgcgtcc agcgcgtacg catcattcgc cgccgtgagg aacatgattc caccgacaac
     2101 aacaggtgtt acttccagat agctggaatt cggcgcatga aaaacccact gcgccgtcag
     2161 ttgcgcgaca ttggcgggag tgatctgccg cagcgcgctg tagcggcgcc ccgtgtagtc
     2221 gccgttgtaa gaaggccagt cagccgccac cgggcgcacc aacaggtgtt cgtttcgcac
     2281 atcaatcgaa gttgaaacca ggccggggcc cggctcttgc gcagcgcata gcaccgcagc
     2341 ccctatcatg gttgccgcgc acgcgagtgc tcgccggcgc atcctgctgg gacggccacg
     2401 ccctgcttct tcatctgaag ccaatgcacc aatcttcctc aaagcgcgag gcacctggca
     2461 aatgtggctt tctatactac aaactgcgga gcgaaaagcc agaaaaatta tagcctgccg
     2521 aattttgcta ctgcttcttc aatcgattta agtctttcga tatgagcgta catagagtgc
     2581 tctttgaagt ccatctcttg cgccagctgc agaacctgcg ccgccacgcc ggccggaacc
     2641 accaccacgc cgtctccgtc cgccacaata aaatcgttcg gattcaccgc cacgccgtcg
     2701 cacgtcaccg caatattggc tccggcaaaa cggtaatgtc ccaccgacgt ggacggcact
     2761 gcccccagcg cgtacacggg aaacccgatt ttctgcaggt aagccgtgtc gcgcactccc
     2821 ccatcaatca ccgcaccgga aaaatttctg gccgacatgg ccgtgcccat cagcccgccc
     2881 atccccgcaa tgtccgcacc gtcttccacc accatcacgt aaaccgagtt tggcccgccg
     2941 gtatcgatgg cgtgcagcat ccctttcagc gcgtcgggat cgtgattcgt ttcctttttc
     3001 aaaagaaccg tcagcgcgtg tccggcgaac cgcgtgggaa aaatcggccg catgcggtgc
     3061 gtcatataca ttttcttgcc ggtgagctgt tcgagtgcgt cggaaatgga agccacttca
     3121 acatggcgaa aaccgtcgag caacgcagcg tcgctgctgg gtgattgtgc cgccgcgcct
     3181 tgtgtagcgc cgcctcttgc tgatgcgaaa aatagcccca gcgtaagcgc cgctgcgccc
     3241 agaaaggcgg cgtggccggg acgacggatt cttctcgaca gaaatgcttt taactgaaat
     3301 tggatgcgca tgattcctcc gcgaaaacga tacgcgcggg cactctagca cctttgcccg
     3361 atagatgcat cgggcatgcg ctctaggtta ttgtttagga gttttaaagg cggctgccca
     3421 acaggagtgt gccgtacgtc gaccgctttc tgcctgggaa aaaggcaggc cttttgagga
     3481 cgcaaaaaaa aacgaggtgc gaatggtctt cgcacctcgt tc
//
LOCUS       DASTPM010000575         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1231308, whole genome shotgun sequence.
ACCESSION   DASTPM010000575 DASTPM010000000
VERSION     DASTPM010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231308"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VFR42_10180"
     CDS             <1..212
                     /locus_tag="VFR42_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369569.1"
                     /translation="VLTINNLVRERFELRLSRASTENSDWCYLPLRHEDWMKLESEFG
                     TDIVKRHLLSLPESWEELETALDAA"
     gene            287..643
                     /locus_tag="VFR42_10185"
     CDS             287..643
                     /locus_tag="VFR42_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0369570.1"
                     /translation="MAKPGPRTTYRYSSDFKATAVRLSRLPGVAVQDVAQSLYIHPFM
                     LSRWRNQAREGLIMTRGVKLDPAIAAELKELRRVKKAYEELKLDHDLLKKAIAFTSAR
                     RATSSPASRSKKEPTR"
     gene            613..1449
                     /locus_tag="VFR42_10190"
     CDS             613..1449
                     /locus_tag="VFR42_10190"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU0369571.1"
                     /translation="MAQQKGTHTVRRMCRVYGVSASGFYAWRERPVSARARQDAVLID
                     KIRRVHRDSRETYGSPRVHAQLCKQGEATGRRRIERVMREHAVVGCSTTLYRRLPGMG
                     RFYASVENKVHGLTIDRPDQVWVGDLTYLKVAGAWRYLAMVMDRYSRRVLGWALGREK
                     TAALARRALASALRLRQPPPGTLFHSDRGVEFMAYDFKQLLTGSGLVQSANRPRTMTD
                     NAHMESWNKSMKSDMYHRHHFSNDRALRTAVRSYIDFYNQQRLHSALGYRSPIEFEAQ
                     CN"
     gene            complement(2214..>3356)
                     /locus_tag="VFR42_10195"
     CDS             complement(2214..>3356)
                     /locus_tag="VFR42_10195"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEU0369572.1"
                     /translation="VGSSAFGDGNTASATDSTAAGFSNTASGIRSSSFGVLNFATGLD
                     ASAFGFDNMASGTMSVAIGVGANASATRSIAIGSWVDKNANGVIDPGEAASASGNDSI
                     AFGNGATANGANDNAIGENSSTLGTNDTAIGGGATVTVSNSVALGAGSIADRANSVSV
                     GSVGHERQITNVAAGTSATDAVNLGQLTAATTMATTALNTASSALTTANYALSESNAQ
                     VTALAGSAGTGAIDAGDAATLVAANAHADAGDAATLSAANAHADAGDATTLAAANSFT
                     TNAVNALNDSFTNFQNHINQELDIQDKRISKMGAMSAAMVQAGINSVGAHGPTGRLSV
                     GAGFQNGQSAISIGWGRPIGEHASFSLGGAFSNGESSAGIGFGVDL"
BASE COUNT          637 a         1007 c         1035 g          677 t
ORIGIN      
        1 tcgttctaac cattaataac ttggtgagag agcgctttga gctccggttg agcagggcct
       61 ccacggaaaa cagcgactgg tgctatctac cgttgcgaca cgaagactgg atgaaattgg
      121 agtcggagtt cggcaccgac attgtcaaaa ggcatcttct aagccttcca gagagttggg
      181 aagaacttga gactgcgctg gatgcagcct aacaattcga tcgagtcgat gtggcggttc
      241 cttcggttta gtggacaccc tgacctaaca atcaggaggt gtccaaatgg ccaagccagg
      301 tccaagaacg acgtatcgtt acagcagtga tttcaaggcg acagcggtgc ggttgagccg
      361 gttgcccggt gtggctgtgc aggatgttgc gcagtcgctt tacatccatc ccttcatgtt
      421 gtctcgttgg cgcaatcagg cgcgcgaggg attgatcatg accaggggtg tgaaactcga
      481 tccggccatt gcggccgaac tcaaggaact gcgcagggtg aagaaggcct atgaggagct
      541 aaagctcgat catgaccttt taaaaaaagc gatcgcgttc acttcggcgc gaagggcgac
      601 atcttcgcct gcatcgcgca gcaaaaagga acccacacgg tgaggcggat gtgccgtgtg
      661 tatggggtga gcgcgagcgg tttctatgcg tggcgcgagc ggccggtcag tgcgcgcgca
      721 cgccaggacg cggtgctgat cgacaagatc cgacgggtgc atcgcgacag ccgggaaacc
      781 tacggcagtc cgcgtgtgca tgcccagctg tgcaagcagg gagaagccac cggacggcgc
      841 cggatcgagc gggtgatgcg cgagcacgcg gtggtgggct gttcgacgac cctttatcgg
      901 cgtcttcccg ggatgggccg gttctatgcc agcgtcgaga acaaggtgca tggactgacc
      961 atcgatcggc ccgatcaggt gtgggtgggc gatctcacct acctgaaggt cgcaggcgca
     1021 tggcgctacc tggcaatggt catggatcgg tattcgcgcc gcgtgctggg ttgggcgctg
     1081 ggacgggaga agaccgcggc cctggcacgc cgtgcattgg catcggcact tcgcctgcgg
     1141 cagccaccgc caggaacgct cttccacagc gatcgcggcg tcgaattcat ggcgtacgac
     1201 ttcaagcagc tgctgaccgg ctccggactg gtgcaaagtg cgaaccgccc gcgcacgatg
     1261 accgacaacg cccacatgga atcgtggaac aagtcgatga aatcggacat gtaccatcgc
     1321 catcacttca gcaacgacag ggcactccgg accgctgtgc gcagctacat cgatttctac
     1381 aaccagcaac gcttacactc ggcgctgggc taccgttcac cgatcgagtt cgaagcacaa
     1441 tgcaactaac aagtgggtgt ccacttttcc gtaggaactc cccgtggttt cgccaggcgg
     1501 cttatttctg gcgttgggtt cactcagata gccctgggag gtagctagtt gagcttcgat
     1561 ctagtcgtgt tcgcacccaa gcacgtccct gagcaggaac gtgcgtttgc tacctggttt
     1621 ggtcagcttc gcgggacagc agacgatttc cgctacccaa actccgagag ctgcgttccg
     1681 gagctgaaag attttttcga tgaacttcga agctatctgc cctggcacga agagaaaccg
     1741 gaacaagagt gttccgcgga ctattcattc gcaaggtaca tgatccactg ctcgttcccg
     1801 tcctcgtgcc agtcgaaagc gttcgacact gttctggctc tgtgctggag attgcagctc
     1861 ggtctctatg accttggcgg cagcggcaaa gtcatttatc cccaatcagc atgggacatc
     1921 atagaagcca ggtccaagtc ccgaagacct tggtggaaat tctggcggcc gtaaacctga
     1981 ccattcgctc aagcggacgc cggaatggat gccgcgattc tcggagcggt gccgcgggcg
     2041 ccgcttggct tggacgtcag gcttcggaac aatcgtctcg aatctgcgtc aggacgtccg
     2101 caagactgca atgtccttac gcatcggtgc atccatccgc cacgtgaccc gcgaccggcc
     2161 atggcgcgga aagcgccgga accccgatcg ggattccggc gcaacgtgca ttcctacagg
     2221 tccacgccga agccgattcc tgccgaactt tcgccattgc tgaatgcgcc gccgaggctg
     2281 aaggacgcat gctcgccgat cggccgaccc cagccgatcg agatggcgct ttggccgttc
     2341 tggaaaccgg cgccgaccga cagtcgaccg gttgggccgt gcgcgccgac cgagttgatg
     2401 ccggcctgca ccatcgctgc gctcattgcg cccatcttgc tgatgcgctt gtcctggatg
     2461 tccaattcct ggttgatgtg gttctggaag ttggtgaagg aatcattgag cgcgttcacc
     2521 gcgttcgtgg tgaacgaatt cgccgccgcc agcgtggtcg catcgcccgc atccgcgtgc
     2581 gcgttggccg ccgacagcgt cgccgcatcg cccgcatccg cgtgcgcatt cgccgcaacc
     2641 agggttgccg catcgcccgc atcgatcgca cccgtacccg ccgaaccggc gagcgcagtg
     2701 acttgcgcat tgctttccga caacgcgtag ttcgccgtgg tcagtgcgct cgaagcggtg
     2761 ttcagcgccg tggttgccat ggtcgtggcg gcggtcaact ggccaaggtt caccgcatca
     2821 gtggcagagg taccggcggc gacattggtg atctggcgct catggccgac actgcccacg
     2881 gacacgctat tggcacgatc cgcgatcgag cccgcaccca gtgcgaccga gttgctgacc
     2941 gtcacggttg ctccgccacc gatcgcggtg tcgttggtgc ccagagtcga gctgttttcg
     3001 ccgatcgcat tgtcgttcgc gccattggcc gtggcgccat tgccgaaggc gatgctgtcg
     3061 tttccgctcg cagacgctgc ctcgcctgga tcgatgacgc cattggcgtt cttgtcgacc
     3121 cagctgccga tcgcgatgct gcgcgtcgcg ctggcattgg cccctacgcc gatggccacg
     3181 ctcatggtgc cgctggccat gttgtcgaag ccgaacgcgc tggcatccag cccggtcgcg
     3241 aagttcagca cgccgaacga actgctgcgg attccactgg cggtattgct gaatcccgcc
     3301 gcagtgctgt cggtcgcgct cgccgtattg ccatcgccga atgcgctgct gccgac
//
LOCUS       DASTPM010000576         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1433446, whole genome shotgun sequence.
ACCESSION   DASTPM010000576 DASTPM010000000
VERSION     DASTPM010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433446"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1444)
                     /gene="tgt"
                     /locus_tag="VFR42_10200"
     CDS             complement(308..1444)
                     /gene="tgt"
                     /locus_tag="VFR42_10200"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU0369573.1"
                     /translation="MNFSFEVLKADAGGARRGRLTTAHGAIETPVFMPVGTQATVKSL
                     RNEALEELGARIILGNTYHLYLRPGHELIRKLGGLHKFMSWDRAILTDSGGYQVFSLS
                     ELRKITNEGVRFRSHLDGSEHLLTPEKAAEIQLALGADIAMVLDECIETPAPRDIAQA
                     AVKRTTAWAKRARAYFLQQAQRNGELSQWQFGIVQGATFTDLRRESAQQLLEIDFSGY
                     AVGGLAVGEPHAVTCEMTAVATELLPREKPRYLMGVGRPEQLADYVALGIDMMDCVLP
                     TRAARHACIYTSEGKVLIKNARYAQDQRPLDPVCSCSVCKRYTRAYLRHLFAAGELTA
                     AILATHHNIHFYLDFMRQIREAIEFGNLAKFSSEVQARYAAGPA"
     gene            complement(1457..>2225)
                     /locus_tag="VFR42_10205"
     CDS             complement(1457..>2225)
                     /locus_tag="VFR42_10205"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEU0369574.1"
                     /translation="GGGITMATTPVVASGAPEAPGPINHFGRLIGVFFSPQATFQDIA
                     ARPSWLVPVLLMTVLGVVTAFVMNQKVDWRDVASKRIEESPRAANLSAEQKEQQLAMS
                     AKVSPYVAYVFGTLGGIGAVVIVAGVMLLAFNVIGGASAKFGTAMGIISHAMVVGLLG
                     TLFFILILFLKPPGTIDLENPVATNIGAFLPESTPKALMALGKSIDIFRIWTLLLISI
                     GFAAVNPKKLAGKAFSIALSVWALYVACKMGAAWIFS"
BASE COUNT          464 a          695 c          600 g          466 t
ORIGIN      
        1 ttcttcaacg cggccagcat ggcttgcgtc tttttgcgct gccgctgctg cggcaggatc
       61 agcatcacgt aaaaaatgcc catgacgata atcatgggca agaacattcc caaaccgctg
      121 cccgctccac ccgtctgcca caagattgct gcgatcatcg ccttaaaagg tcttccttat
      181 tgaagtcacg tctcaccggg aatactgacc attcgccaag ggcgagttac ttcaccctcg
      241 cgttccccgc cgattcaatt cactgtgcga ttctccgagc ggccagacgt gcaacccgcg
      301 cggcgcttta cgcgggaccc gctgcgtaac gtgcctgcac ctcggacgag aatttcgcta
      361 gattcccaaa ctcgatagcc tcgcggattt gcctcatgaa gtcaaggtaa aagtggatgt
      421 tgtggtgggt ggccaggatc gccgcagtga gttctcccgc ggcgaacaga tgccgaagat
      481 acgcgcgggt atagcgtttg cagacgctgc aggagcacac cggatcgagc ggccgttggt
      541 cctgcgcgta ccgcgcgttc ttgatgagga cttttccttc gctggtgtag atgcacgcgt
      601 gacgggctgc acgtgtgggc agcacgcagt ccatcatgtc gatgcccagc gcaacatagt
      661 cggctagctg ttccgggcgt cccacgccca tcagatagcg cggcttctcc ctcggcaaaa
      721 gttcggtcgc gactgcggtc atttcgcaag tgacggcgtg cggctcgcca accgccagtc
      781 cgccgaccgc ataaccggaa aaatcaattt ccaggagttg ctgtgcgctt tcgcggcgca
      841 agtcggtgaa cgtcgcgcct tgcacgattc caaattgcca ttgcgacagt tcgccgttcc
      901 gctgtgcttg ctgtagaaaa tatgcgcgcg cgcgtttagc ccaggccgtc gtgcgcttca
      961 ctgcggcttg cgcgatgtcg cgcggcgccg gcgtttcgat gcactcgtcc agcaccatcg
     1021 cgatgtccgc acccagcgcc agctgaattt ccgccgcttt ttccggagtc agcaaatgtt
     1081 ctgacccatc cagatgcgaa cggaaacgca cgccttcatt ggtgattttg cgcaactcgg
     1141 aaaggctgaa gacctggtag ccgccggaat cggtcagaat cgcgcggtcc cacgacatga
     1201 atttgtgtaa tccgcccagc ttgcggatca gctcgtgtcc cggccgcaga tataaatggt
     1261 aggtattgcc gaggatgatg cgcgcgccga gttcttccag cgcttcattg cgcaggcttt
     1321 tgactgtcgc ctgcgtgccc accggcatga acaccggcgt ttcgatcgct ccatgcgcag
     1381 tcgtcagccg tccccgacgc gcgccgccgg cgtccgcctt caaaacctca aacgaaaaat
     1441 tcactcatcg agtgtatcag gagaaaatcc acgcggcgcc catcttgcag gcgacataca
     1501 gcgcccagac cgacagcgcg atgctgaaag ctttgcccgc aagcttctta ggattcaccg
     1561 ccgcgaatcc gatcgagatc agcagcaacg tccagatgcg gaaaatatcg atggattttc
     1621 cgagagccat cagcgctttg ggcgtgctct ccggcagaaa tgctccgatg tttgtagcca
     1681 ccggattctc gaggtcgatg gtgcccggtg gcttcagaaa aaggatcagg atgaaaaaca
     1741 gtgtgcccag cagtcccacc accatcgcgt gcgaaatgat acccatcgcg gtgccaaact
     1801 tcgcgctcgc tccgcctatc acgttaaatg ccagcagcat cactccagcc acgattacca
     1861 ccgcgccaat ccctcccagc gttccgaaaa cgtacgccac ataaggcgaa acttttgcgc
     1921 tcatcgccaa ttgctgttct ttttgctccg cgctcagatt ggctgcgcgc ggactctctt
     1981 caatgcgttt actcgccacg tcgcgccagt ctactttctg attcatcacg aacgctgtca
     2041 ccacgccgag cacggtcatc aaaagaaccg ggaccagcca gctaggccgc gcagcaatat
     2101 cttgaaaggt ggcctgcggc gaaaagaata cgccgattag acggccgaaa tgattaatgg
     2161 gaccgggcgc ttccggagct ccggaagcta cgacaggggt cgttgccatc gtaattcctc
     2221 cacca
//
LOCUS       DASTPM010000577         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_827722, whole genome shotgun sequence.
ACCESSION   DASTPM010000577 DASTPM010000000
VERSION     DASTPM010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827722"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VFR42_10210"
     CDS             complement(<1..1741)
                     /locus_tag="VFR42_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0369575.1"
                     /translation="MSKLSPDEWRALSPHLDEALEMTEDQRSTWLSSLQTVNPILASQ
                     LELLLQEHRLLSEKGFLEAASMELPGKVARAGQTFGAYTLVTQIGHGGMGTVWLAQRN
                     DGRFERRVAVKVLNVALMGKGGEERFRREGRILGRLTHPHIAELIDAGVSPDGQPYLV
                     LEYVEGDHIDRYCDQMKLDVRARVRLFLDALHAVAEAHAKHIVHRDLKPSNLLVRVDG
                     QAKLLDFGIAKLLEAEGQIGESPITLEGARVLTPEYAAPEQLKGEAVTAATDLYAAGV
                     LLYVLLTGQHPAGAGPHTPARLIEAILHTEPLCPSATVAQTEIKQETTAANAQRRATT
                     PDQLARLLRGDLDTIVGKALKKNPRERYVSIEAFAEDLQRYLRYQPIAARPDTLPYRT
                     GKFIRRHRSSVTAALLVSLALMGIAILTWLFPRRPEALPQFNQRKLTANAQDSPVLSA
                     AISPDGKYLGYVDQQGIHLQVVETGVVQSVAQPPEFAPGRASLGFGSWFPDSTRFIVS
                     VSGIGKPSTVWSIPITGGEIQKLAEVEDILGGGIVSPDGLKVAYQRLRVTSDEAREIW
                     IMGANGESPHKIFT"
     gene            complement(1749..2393)
                     /locus_tag="VFR42_10215"
     CDS             complement(1749..2393)
                     /locus_tag="VFR42_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU0369576.1"
                     /translation="MAALVAAGKCATLWALLGDSPAGVCRVESTISALIGAAESGDCV
                     AADTLFSTLYSELHRLAKRELARRGSPASLSVTTLLHEAYLDIAAREGHCFPDRPRFM
                     GYAARVMRGLIIDHARSRNAIKRGGEFEITSLKTDVDQNSVDAKELSLISDALDQLAK
                     VEPKLAELVDMKFFCGFSFAEIAALENLSERTVQRKWEKARIYLHRNIRADLPL"
     gene            2504..3718
                     /locus_tag="VFR42_10220"
     CDS             2504..3718
                     /locus_tag="VFR42_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU0369577.1"
                     /translation="MSLWSRRKFFLTSLAGTAAAGTTNLVGATGASRRSTNGSGLSSL
                     FLEAASKGQRPVMISSANGVHALQKGMDILKSGGDTLEAAVAAVSVVEDDPNDDSVGY
                     GGLPNEEGEVELDASVMHGPSRRAGSVASVRRIKNVSRLAKTVMERTNHVMIVGDGAR
                     RFAVAEGFEEMNLLTESSRKKWLAWKAATSFNWRPGIDSPEWSGKTASRSKSPLDSDE
                     FRARLADICGNDEALMARALEVIKDPPSGTINCLAVNDKGEISGTTTTSGLSWKIPGR
                     VGDSPIIGAGLYVDGDVGGAGSTGKGEENIKISGGHTIVEMMRKGMSPTDACLEALHR
                     VARNYNNDKKKLGTFHIYFYALNKDGVHGSASLWRNGYDPNKRAKYAVHDGTQGHLED
                     CAAYFDEVSGGE"
     gene            complement(3724..>3908)
                     /locus_tag="VFR42_10225"
     CDS             complement(3724..>3908)
                     /locus_tag="VFR42_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEU0369578.1"
                     /translation="LIKYLAQAESISEAARTIEQGGLEVDGIVINDPRAKLDTTFDAM
                     YEVRFGKKKFFRLIVE"
BASE COUNT          828 a         1144 c         1136 g          800 t
ORIGIN      
        1 ctgtaaaaat tttgtgagga gactctccat tcgcgcccat tatccagatc tcccgcgcct
       61 catcagaggt gactcgcagc ctttggtagg cgactttgag cccgtccggt gagacgatcc
      121 cgccgcccaa aatatcctca acttcagcaa gtttctggat ctcgcctccg gttatcggaa
      181 ttgaccacac tgtggatggc ttcccgatgc cgctgacaga gacaatgaat cgagtggagt
      241 ccgggaacca actcccaaac cccaatgagg cccttcccgg cgcgaactcg ggaggctgag
      301 ccacgctttg gactacgccg gtctctacga cctgcaaatg aatgccttgc tggtcaacgt
      361 agcctaagta cttgccatcc ggagagatcg cagcactgag caccggtgag tcctgggcgt
      421 tcgccgtcag ctttcgctga ttgaactggg gtagagcttc ggggcggcgg ggaaagagcc
      481 acgttaagat ggcaatgccc atcagcgcca gtgataccag cagcgccgct gtcacactac
      541 tcctgtgacg acgaatgaat ttccccgtgc gatagggcag tgtgtcgggc cgagccgcga
      601 tgggctgata tcgtagatag cgctggagat cttccgcaaa ggcttcgatg gagacatatc
      661 gctcgcgcgg attcttcttc agcgctttcc caacaatggt gtcgaggtca ccgcgcagca
      721 accgggccag ctggtcgggg gtggtggcgc gtctttgggc attcgctgcg gtagtctctt
      781 gttttatctc ggtctgagcc acggtcgccg aagggcagag gggctcggtg tgcaagatag
      841 cttcgatcag acgtgccggc gtgtgcggac cggccccagc cgggtgctgt ccggtcaaca
      901 gcacatacag caggaccccc gcagcgtaga gatccgtggc cgcggtcaca gcttcgcctt
      961 tcaattgctc gggagccgca tactcggggg tcagaaccct tgcgccttcc agagtgatcg
     1021 gagactcccc gatctgtcct tctgcttcca gaagtttggc aattccaaag tcgagaagct
     1081 tggcttgacc atcaactcgc accaacaggt tcgatggctt cagatcacgg tgcacaatgt
     1141 gtttggcgtg agcttccgcc acggcgtgca aggcgtcgag gaatagccga acgcgcgccc
     1201 gaacgtcgag cttcatttgg tcgcagtagc ggtcaatgtg atcgccctca acatactcga
     1261 gcaccagata aggctggcca tcgggcgaca ctcccgcgtc aatcagttcc gcgatatggg
     1321 gatgtgtcaa ccggccgaga atgcgccctt cgcgtctaaa ccgctcctcg ccgcctttcc
     1381 ccatcaaggc cacattaagt accttcaccg ccacgcgccg ctcaaaacgc ccgtcgttcc
     1441 gttgtgccag ccagacggtg cccatgcctc cgtggccgat ttgcgttacc agcgtgtaag
     1501 cgccaaaggt ttgtcctgca cgggcaactt tacccggcaa ttccatggaa gccgcttcga
     1561 gaaaaccctt ctcagacagc aggcgatgct cctgaaggag caattccagt tgcgatgcca
     1621 gaattgggtt caccgtttgc agggaggaca gccaggtgga gcgctggtcc tccgtcattt
     1681 ccagcgcttc gtcgaggtga ggactaaggg cccgccattc atccgggctg agcttggaca
     1741 tcaatacctc agagtggcaa atccgcgcga atgttgcgat ggagatagat gcgcgccttc
     1801 tcccacttcc tttgtaccgt gcgttccgac aagttctcca gggccgcaat ttccgcgaaa
     1861 gaaaatccgc agaaaaactt catgtccacc agttcggcta gcttcggctc gactttcgcc
     1921 agctgatcca gggcgtcgct gatcaacgag agttccttgg cgtcgacgga attttggtca
     1981 acatccgtct tgagcgaagt gatttcgaat tcgccgccgc gcttgattgc atttcggctc
     2041 cgggcatgat caatgatcaa tccgcgcatc acccgtgcgg cataccccat gaaccgcggc
     2101 cggtcgggaa agcagtggcc ctcacgcgcg gcgatgtcga gatacgcctc gtgcagaagt
     2161 gtggtaacgc tcaggctcgc tggggatccc cgtcgcgcca gttctctttt tgccagacgg
     2221 tgcaattcgg agtacagcgt gctgaaaagt gtatccgcgg ctacgcaatc gccgctttca
     2281 gccgcgccga tgagtgcgga aattgtcgac tccacacggc acactcctgc cggcgaatcg
     2341 cccagaagtg cccacagtgt cgcacatttg ccagctgcga ccaaggcagc caagcccttc
     2401 ctagtgtcgc aacaacttgc tgcgactgcc gctcgggatg caagcaagaa agccgcagtg
     2461 tatagtggcg gcctcgcttt gacttgtcct cgtggaggga cgcatgagcc tgtggtcacg
     2521 ccgcaaattt ttcttaacct cgctggccgg gacggctgcc gccggcacca ccaatttggt
     2581 gggcgcaact ggggcctctc gacgctccac gaacggctca ggcttgagtt cgctttttct
     2641 cgaggccgct tcgaaaggcc agcggcccgt catgatttcc agcgcgaatg gagtccatgc
     2701 cctgcaaaag ggcatggaca tcttgaaaag cggcggcgac acactcgagg cagcagttgc
     2761 tgcagtcagc gtggttgagg acgatcccaa tgatgattcg gtgggctacg gcggattgcc
     2821 gaacgaagag ggtgaagtgg agctcgacgc aagcgtgatg cacgggccta gtcggcgcgc
     2881 tgggtcagtg gccagtgtac gcagaatcaa aaacgtgtcg cgcctggcaa aaaccgtgat
     2941 ggagcgcacc aatcacgtaa tgattgtcgg cgacggcgca cgccgcttcg cagtagcgga
     3001 aggcttcgaa gaaatgaacc tgctcaccga gtcttcacgc aagaagtggc tggcctggaa
     3061 ggcggcgacg tcatttaact ggcggccggg cattgacagt ccggagtgga gcgggaaaac
     3121 ggcgtcacgc agcaagagcc cactggactc ggacgaattc cgcgcccggc tggccgacat
     3181 ttgcggcaat gacgaagccc tcatggcgcg cgccctcgaa gtgatcaagg atcctccatc
     3241 cggcaccatc aattgcctcg ccgtcaacga caaaggagaa atttccggca ccaccacgac
     3301 ctcggggcta tcctggaaaa ttccggggcg cgtgggagat tcgccaatta ttggcgcggg
     3361 cttatacgtt gacggcgacg tcggcggcgc tggctcgacg ggaaaaggcg aagagaacat
     3421 caagatttct ggcggtcata cgatagtgga aatgatgcgc aagggcatgt cgcccaccga
     3481 cgcctgcttg gaagcgctgc atcgcgtcgc acgcaactat aacaatgaca agaagaagct
     3541 tgggactttt cacatttatt tctacgcgtt gaacaaagac ggcgtgcacg gaagcgcctc
     3601 gttgtggcgc aatggctacg accccaacaa acgtgccaag tacgccgttc acgatggcac
     3661 ccaggggcac ctcgaagatt gcgcagcgta ttttgacgaa gtcagcggcg gcgaataagg
     3721 cgctcactcg acgattaagc gaaagaattt cttcttgccg aatcgtactt catacatcgc
     3781 atcaaaggtt gtatcgagtt tggccctggg atcgttgata actataccgt ccacttctag
     3841 gccaccctgc tcaatggttc ttgcggcctc cgagatagac tccgcctggg ctagatactt
     3901 gatgagtt
//
LOCUS       DASTPM010000578         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_193561, whole genome shotgun sequence.
ACCESSION   DASTPM010000578 DASTPM010000000
VERSION     DASTPM010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193561"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFR42_10230"
     CDS             <1..806
                     /locus_tag="VFR42_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0369579.1"
                     /translation="HKAEVLVGAGDIADCTNLAGAEATAALLAKIPGTVMAVGDLAYP
                     DGTPANFACYDKTWGREKSRTRPAVGNHEFHSAGATFYFQYFGEAAGDSSRGYYSYEL
                     GAWHIVVLNSECLQVGGCGPGSAQEKWLRDDLAAHPVACTLAYFHKPLFSSGGAHGDD
                     LTVKPLWDALYHAGAEVVVNGHDHDYERFAPQTPEAKADPAHGIREFVAGTGGKNHRP
                     FGASRPNSEVRNADAFGVLKLTLLPGKYAWEFVPEEGKTFADSGEGVCH"
     gene            complement(846..>2063)
                     /locus_tag="VFR42_10235"
     CDS             complement(846..>2063)
                     /locus_tag="VFR42_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0369580.1"
                     /translation="LTRKRNRAIDEIITDVRNEINKTEPATRVEFVQVLQDMIGDLSN
                     EPEPVVINLFSQDPQLLLTAAPRVADAIGKVDGVVDVLNGVENTISGPASTFRVNTAT
                     AARSGFTPEEIAVDASAILEGEPAATPVVLNDRVYPIRVRFPDSSRASLEQMMNTPIL
                     SATGRTATLGSLASLTNDPGQTETRRENSQRYMRVTARLEGVGLGSGMAAVQKAVNGL
                     HLPSSIRVEYGGTYKEQQKSFHDLLQVLVLALLLLFIVLLFEFRSFSAPAAILASALL
                     STFGALFALLITRTTFNIASFMGVIMVVGIVAKNGILLLDADQRFRAFGLSAEDAMIQ
                     AGRRRLRPIAMTALATVAGMLPLAFGLGAGSEMLKPLAITVVGGLLSSMVLSLVFTPA
                     IHYLLHREKTHPA"
BASE COUNT          439 a          523 c          706 g          395 t
ORIGIN      
        1 cgcacaaagc agaggttctt gtgggagccg gcgacatcgc tgactgcacg aatcttgcgg
       61 gtgcggaagc gacggctgcg ctgctcgcga aaattcccgg tacggttatg gccgtgggcg
      121 atttggcata cccggacggg acgcccgcca actttgcgtg ttacgacaag acatgggggc
      181 gggaaaagag ccggacgcgg ccagccgtag gcaaccacga gtttcattcg gccggagcta
      241 cattctactt tcaatacttt ggtgaagcgg ctggggactc gagcaggggt tattacagct
      301 atgagctcgg ggcgtggcac atcgtggtat tgaacagcga atgtttgcaa gtgggtggat
      361 gtgggccggg atcggcgcag gagaaatggt tgcgcgacga cttggctgcg catccagtgg
      421 cgtgtacgct ggcctatttt cacaaaccgt tgtttagttc gggcggagcg cacggcgacg
      481 acttgacggt gaagccgctg tgggacgctc tgtatcacgc aggtgccgaa gtggttgtaa
      541 acgggcacga tcacgactac gagaggtttg ctccgcagac cccggaggcc aaggccgatc
      601 cagcgcacgg gatacgcgag ttcgtggctg ggacgggcgg aaagaatcac cggccgtttg
      661 gcgcgtcacg ccccaacagt gaagtacgca acgcggatgc gtttggagtg ctgaaactga
      721 cgctgctgcc cgggaaatat gcttgggagt ttgtgcccga agaagggaaa acgttcgcgg
      781 actcgggcga gggggtctgc cactaagctg ccgaaataag atcacggcgc ctttgcagat
      841 gcacgctaag cggggtgcgt tttttcgcgg tgcagcaagt agtgaatcgc gggtgtgaag
      901 accagcgaca ggaccatcga gctgagcagc ccacccacca cggtgatggc aaggggcttg
      961 agcatttcgg agccggcgcc gaggccgaag gcaagcggca gcattccagc gaccgtggcg
     1021 agagcggtca tggcgatggg gcggagccgg cggcggcccg cttgaatcat ggcatcttca
     1081 gcggacaggc cgaatgcgcg gaagcgctga tcggcgtcga gaagcaaaat accgtttttg
     1141 gcgacgatgc ccacgaccat gatgacgccc ataaacgatg caatgttgaa cgtagtgcgt
     1201 gtgatgagca gagcgaagag cgcgccgaac gtcgaaagca gagcggaggc aagaatcgca
     1261 gcgggtgcgg agaagctgcg aaattcgaag agcagaacaa tgaagagcag tagcagcgcc
     1321 agcaccagca cttgcagcag gtcgtgaaac gatttttgtt gttccttgta ggttccgccg
     1381 tattcgacac gaatggaaga aggcaagtgt aggccgttga cggccttttg taccgctgcc
     1441 atgccggaac caagcccgac tccttcgaga cgtgcagtca cccgcatgta gcgctgcgaa
     1501 ttctcgcgcc gggtttccgt ctggccggga tcattcgtca gtgaggcgag agagccgagc
     1561 gtagcggtgc gtcccgtcgc gctgagaatg ggcgtgttca tcatctgttc gagtgaagcg
     1621 cggctggagt cggggaagcg gacgcgaatg ggatagacgc gatcgttcaa cacgacgggt
     1681 gtcgccgcgg gttcgccttc caaaatggcg gaagcgtcga cggcgatttc ttctggggtg
     1741 aaaccggaac gcgccgcggt tgcagtgttg acgcgaaatg tggaagccgg accgctgatg
     1801 gtgttttcga cgccgttgag gacgtcgact actccgtcga ccttgccgat tgcgtccgca
     1861 acgcggggtg cagcggtgag caacaactgg ggatcctgcg agaagagatt gataaccacc
     1921 ggctcgggct cattgctcag atcgccaatc atatcctgca gaacctgaac aaattcgacg
     1981 cgagtagcgg gttcggtctt gttaatttcg ttgcgcacgt cggtgatgat ttcatcgatg
     2041 gcgcggttgc gttttctcgt tag
//
LOCUS       DASTPM010000579         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_366640, whole genome shotgun sequence.
ACCESSION   DASTPM010000579 DASTPM010000000
VERSION     DASTPM010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366640"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFR42_10240"
     CDS             <1..205
                     /locus_tag="VFR42_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEU0369581.1"
                     /translation="FYSHEPNEPPHVHVNRDELSAKFWLTPVALARNFGFTPVELRKV
                     QSLVEDHQRHFLEEWNGHFGLEG"
     gene            527..1699
                     /locus_tag="VFR42_10245"
     CDS             527..1699
                     /locus_tag="VFR42_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEU0369582.1"
                     /translation="MRIFVLGAGATGSLLAQLLHRQGHTVWCGDRDLQRAHHFLGKNT
                     SIAITDVNARNLRGIVRAARGCQLLINASASVFNQIVMRAALRLRVHYLDLSSHLTQN
                     PFKAEQLAFAKRFAQKKRLALINTGAAPGLTNLLVKRATEMLDEVETVQIRLYESTES
                     DDPISQWSPEVTFDEAISNPRIYRNGRFAMARRFAEREKFRFPDPVGAANVVLAAQDE
                     VATLPYFISMKNMDVKIGGNEFDRLRRWYKQGKLSKSRGIVRHRFPQTLSPRKIASLI
                     RQGVLQNARFAAVVLATGIKRRSKQEERRLIRTECLFPTLYQIRQQGLYTTPVAYATA
                     HVAALFVKFFPRDLAGVFPPEALPVQTRRAIVAGIRTHSVRIQHKTTALKPDGDDF"
     gene            complement(1720..2514)
                     /locus_tag="VFR42_10250"
     CDS             complement(1720..2514)
                     /locus_tag="VFR42_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369583.1"
                     /translation="MRRAPVFAAIVLLTFSAGRVAAAAPDSPESNAADASAEPSPAPP
                     AKGSSEGSFPIGVGVKVSTLGVGGEVAVAVSHRTNIRVGFNAFSYGHTFDKDGATYKG
                     NLNLRSAQATWDLFPVRWFHVSPGVLLYNGNQVTANVSVPGGQTFTLSNTSYISDAAD
                     PINGTGKLSVYKTAPMVLFGIGNLVPRGGHFSSSFEIGAAYQGPPRVALNLAGSACDS
                     TGVFCRPVGTDPTIQSNIVAEQGKLNKSASPYKFYPVISFGFGYRF"
BASE COUNT          573 a          913 c          743 g          548 t
ORIGIN      
        1 cttctatagc catgaaccaa acgagccacc tcacgttcac gtgaatcgcg atgagctctc
       61 tgcaaagttt tggctcacgc cggttgcatt ggctcgaaat ttcggcttca cacccgtcga
      121 actgcgtaag gtacaatcgt tggtagaaga tcatcagcgc cattttctgg aggaatggaa
      181 tgggcatttt ggacttgagg gctgacgagc gcgtcgcaaa cgtcacgttt tcacccgact
      241 ccctctctgt gtccctcaag gatggccgct cgatctctgt tcctctaacc tggtacccca
      301 agcttctgca tgccaccgcg gaacagcgaa gaaattggaa gattagcggc ggtgggtacg
      361 gtatccactg gccggatatc gatgaagacc tgagcaccga aggccttctt cgaggagcgc
      421 ccgcggccca tccgcccgta aagtcctgat tacaagcttc tggaaaccag ggcgaaaccc
      481 gctggccgtt ccctcccgcg ggctatggta gagtttttgc cacgccatgc ggatcttcgt
      541 tctcggggcc ggcgccaccg gctctctcct ggcccaacta ttgcaccggc aagggcacac
      601 ggtctggtgc ggggaccgcg acctgcagcg ggctcaccat ttcctgggca aaaataccag
      661 tatcgccatt accgacgtca acgcccggaa cctgcgcggc atcgtgcgcg ccgcccgcgg
      721 ctgccagctg ctgatcaacg cttcggcctc cgttttcaat caaatcgtga tgcgtgcggc
      781 gctgcgactg cgcgtgcatt acctggacct gagctcgcac ctgacgcaaa accccttcaa
      841 ggccgagcag ttggccttcg ccaaacgctt tgcacagaaa aaacggctgg ccctcatcaa
      901 caccggcgcc gcccccggcc tgaccaacct gttggtcaag cgcgccacgg aaatgctcga
      961 tgaagtggaa accgtgcaga ttcgcttgta cgaaagcacc gagagcgacg atcccatttc
     1021 ccagtggtcg ccggaagtta cgttcgacga agccatatcc aacccgcgca tctaccgcaa
     1081 cggccggttc gccatggcca ggcggttcgc cgaacgcgaa aaatttcgct ttcccgatcc
     1141 ggtaggcgcc gcgaacgtgg ttcttgctgc gcaggacgaa gtggccacgc tgccctactt
     1201 tatttccatg aaaaacatgg acgtgaaaat tggcggcaat gaattcgacc gcctgcgccg
     1261 ctggtacaag caggggaaac tctcaaaatc gcgcggcatt gtgcgccatc gcttcccgca
     1321 aactctgtcg ccgcgcaaga ttgcttcctt aattcgccag ggcgttttgc agaatgcgcg
     1381 tttcgccgcc gtcgtgctgg ccaccggcat caagcgccgc tccaagcagg aagagcgccg
     1441 cctgattcgc accgaatgcc tgttcccgac gctgtaccag atccgccagc agggcctgta
     1501 caccacgccg gtggcctacg ctaccgcgca cgtcgcagcg ctgttcgtca aatttttccc
     1561 gcgcgatctt gccggggtct tcccgcccga ggctttgccg gtgcaaacgc gccgcgccat
     1621 cgtcgccggc atccgcaccc acagcgtgcg catccagcac aaaacgacgg cgctcaagcc
     1681 cgacggcgac gatttttgat tttggtgaag agcccggacc tagaatcggt atccgaaccc
     1741 gaacgatata accggataaa atttgtaggg cgaagcgctt ttattcagct tgccttgctc
     1801 cgccacgata ttgctttgaa tcgtcgggtc cgttccgacc ggccggcaaa acactccggt
     1861 cgaatcgcaa gcgctgcctg ccaggttcag cgccacccgc ggcggccctt gatacgccgc
     1921 gccaatttca aagctgctgc tgaaatgccc gccgcgcgga accagattgc cgatcccgaa
     1981 cagcaccatc ggcgccgtct tatacacgga aagcttcccg gtcccgttga tggggtccgc
     2041 ggcatcgctg atatagctgg tattgctcaa ggtgaaagtc tgtccgcccg gcaccgacac
     2101 attcgcggtc acctgattgc cgttgtacaa cagcacgccg gggctaacgt gaaaccagcg
     2161 caccggaaac agatcccaag tggcttgcgc cgaacgcaga ttcagattcc ctttgtaagt
     2221 cgcgccgtct ttatcaaacg tgtgcccgta actgaacgcg ttgaacccca cgcggatgtt
     2281 ggttcgatgc gataccgcca ccgccacttc gccaccgact cctaaagtcg atactttcac
     2341 gcccacgcca atcggaaagc ttccctccga cgatcctttg gccggcggcg ccggacttgg
     2401 ctccgccgaa gcatcagccg cattcgattc cggggaatcc ggcgcggccg ctgccaccct
     2461 gcccgccgaa aatgtcagta acacaattgc cgcaaacacc ggggctcttc gcatggtctc
     2521 tcctcaagat caccgtagca tccgcgaaga gcgctcttct cttaatagct gctaatactc
     2581 gacaaaaggt acccggccct tggtacaggg aaacaaaaaa tcttgattgc cccgcgaaag
     2641 ccaaatgtca gcagcagcgg ttgggtcgcg agtaacgccg atcgagctgc tgcacgtaaa
     2701 attcttccgc gctgagccgc tgccgccctt cgcctcgcgc cgccggcgac cgcaggtagc
     2761 gttcatacgc cgcgtcc
//
LOCUS       DASTPM010000580         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_280548, whole genome shotgun sequence.
ACCESSION   DASTPM010000580 DASTPM010000000
VERSION     DASTPM010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280548"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFR42_10255"
     CDS             <1..342
                     /locus_tag="VFR42_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEU0369584.1"
                     /translation="GIRFVSIPVSGWSPPTNDQVAAFLALFRDHPKEKVFVHCRFGDD
                     RTGVFVATYRMAYEGWPAQQAINEMYFFGFNGFWHPSMKAFIRDFPARLKSAPALASF
                     HPANAPAAPRP"
     gene            481..1377
                     /locus_tag="VFR42_10260"
     CDS             481..1377
                     /locus_tag="VFR42_10260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0369585.1"
                     /translation="MQVGRYQLFETLGVGATSRVVRGFDPMIGRPVAVKIFPSEIAQG
                     EARDRFLREARVVGQLSHPNIITLLDMGIEETSQTPYLVMEFIDGVPLDRILEKGTLP
                     LPRACAWIAHAAEALDVAHKRGVIHGDVKPANILITADGRVKLTDFGMARVARREARD
                     SGLLGTPAYWCPEQIMGRAQDARSDIFSLGVVLYEMITGARPFDADSLQGICNRVLSS
                     TPNAVSQLQPSIPAGFDEIIAGCLAKAPESRIASGEALAQLLYPHARRKVVLPQQAAP
                     AKPAAPAQPSLRDRASRLLRSA"
     gene            1566..>2305
                     /locus_tag="VFR42_10265"
     CDS             1566..>2305
                     /locus_tag="VFR42_10265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0369586.1"
                     /translation="MSQTRYQTLGTIGVGASSRVDKAHDTLIDRTVVLKTFATGFGSP
                     ELQKQFLREAQILGRLSHPNIISIYDLGTNDDGSAYLVTEYVPGKTLDAVLAEKGPVP
                     LARAGVWAGDLASALSRAHKAGVIHGDVKPANIFVTDDGQIKLGDFGIARFATQVSGA
                     ASLVGTPAYLSPEQIKGDPQDHRSDIFSVGIVLYQLTTGIRPFDGSSIAAVCAQIVAK
                     QPAPPSHHNPTLPLEFDRIVMRCLAKNPA"
BASE COUNT          423 a          835 c          553 g          494 t
ORIGIN      
        1 ggcattcgtt tcgtcagcat tcctgtcagt ggatggtctc ctccgaccaa cgaccaggtc
       61 gccgccttcc ttgctctgtt tcgcgaccat cccaaagaaa aagttttcgt ccattgccgt
      121 tttggcgacg atcgcaccgg cgttttcgtc gccacctatc gcatggctta cgaaggctgg
      181 cccgcgcagc aagccatcaa cgaaatgtat ttcttcggat tcaacggctt ttggcatccc
      241 tccatgaaag cgttcattcg cgattttccg gctcgcctga agagcgctcc cgccttggcg
      301 tcgtttcatc cggcaaacgc tcccgcagca ccgcggcctt aggataactg cattattttt
      361 ctataagtta gaggagcgtt tctcctagta ccatcgtttc ccgtttttcg ggactttcgc
      421 tccattttgt accgcccagc ctcgacccta tgcttccgca agaacggaaa ggttgtcccc
      481 atgcaggtcg gtcgctacca gctctttgaa actctcggcg tcggcgccac cagccgcgtt
      541 gttcgcggct tcgatcccat gatcggccgc cccgtggccg tcaaaatttt cccctccgaa
      601 attgcgcaag gcgaagctcg cgaccgcttc ctgcgcgaag ctcgcgtcgt cggccagctc
      661 tcccatccca acatcatcac gctgcttgac atgggcatcg aagaaacctc acaaacgccg
      721 tatctcgtca tggaatttat tgacggcgtt ccactcgatc gcattctcga aaagggcaca
      781 ctgccacttc ctcgcgcctg cgcttggatc gcgcacgcgg ctgaagcgct cgacgtcgcg
      841 cacaagcgcg gcgtcattca cggcgacgtc aaacccgcca acattctcat caccgcggat
      901 ggtcgcgtga agctcactga ctttggcatg gcccgcgtcg cccgtcgtga agcccgggac
      961 tccgggctgc tcggcacgcc ggcttattgg tgccccgaac agatcatggg ccgcgcgcag
     1021 gacgcccgct ccgacatctt ttctctcggc gtcgttctct acgaaatgat tactggcgcc
     1081 cgccccttcg acgccgactc tttgcaaggc atctgcaatc gcgttctgtc ttccacgccg
     1141 aacgccgtct ctcagctgca gccttccatc cccgcgggct tcgacgaaat catcgctggg
     1201 tgcctggcca aagcgcctga gtcccgcatc gcgtccggcg aagccctggc gcagctgctc
     1261 tatccgcacg cgcgccgcaa agtcgttctt ccccagcaag cggcaccagc taagcctgcc
     1321 gccccagccc agccttcctt gcgcgatcgc gcctcacgcc tcctacgctc cgcctgagcg
     1381 cagcgccggc atactgcagg ctcttcgcgc ggggcgaaag tccgtcgaag ccagcgctga
     1441 cagaagaatt ttcgtactaa gggcgccacc ccatttttgt aacttttgtc ccattggccc
     1501 gcgcacttcc ctcttgcaat ctggtcatgg cgcgacgcta tccgccacaa ccgaaaacaa
     1561 catccatgtc ccagacccgc taccagactc ttggcacgat cggcgtcggt gcctccagcc
     1621 gtgtcgacaa agcccacgac actctgatcg atcgcacagt ggtgctaaag accttcgcca
     1681 ccggattcgg ctcccccgaa ctccaaaaac agttccttcg cgaagcgcaa atcctcggcc
     1741 gcctttccca tcccaacatc atttccatct acgatctcgg caccaatgac gacggctccg
     1801 cctatctcgt caccgaatac gtccctggca aaactctcga tgcggtcctg gcggaaaaag
     1861 gtcccgtacc tctcgcccgc gccggcgttt gggctggcga tctcgccagc gcccttagcc
     1921 gcgcccacaa agctggcgtg atacacggcg acgtcaagcc cgccaacatc tttgtcaccg
     1981 acgatgggca gatcaaactc ggcgacttcg gcatcgcccg ctttgccact caggtttccg
     2041 gcgccgccag tctggtcggc acacccgcct atctctctcc cgaacagatc aagggcgacc
     2101 cacaggacca tcgctccgac attttttccg tcggcattgt tctctatcaa ttgaccacag
     2161 gcatccgccc cttcgacggc tcctccatcg ccgcagtctg cgcccaaatc gtcgccaagc
     2221 aaccggctcc gccttcgcat cacaatccca cacttcctct ggaattcgat cgcatcgtca
     2281 tgcgctgcct cgccaagaat cccgc
//
LOCUS       DASTPM010000581         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_943233, whole genome shotgun sequence.
ACCESSION   DASTPM010000581 DASTPM010000000
VERSION     DASTPM010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943233"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1953)
                     /locus_tag="VFR42_10270"
     CDS             complement(<1..1953)
                     /locus_tag="VFR42_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0369587.1"
                     /translation="MTRTNLNSLADSFLRFRNDGAVVETRGYRRHLLTYAELHAAAEN
                     WSAVLEKEGIGAGDRVLLWGANSAGWVSAFWGVLRRGAVAVPMDAGASLDFVRRTVAQ
                     SGAKLILRESRGEEISGKVRTLWLSERRSIEARRSGERRSDAGDCSVGREAIAEILFT
                     SGTTAEPRGVVLTHGNFLANLEPIERAMLPYLKYEKWLHPLRFVSLVPLSHVFGQFMG
                     LFVPPLFGAKVVFENSANPADIVRTLKREKATLLIAVPRMLDLVRVQMEREIDARGWR
                     KWFDRNYEAASGESFLRRAWRFRRIHRLLGFRFWAFISGGAALGATTELFIKRLGFAV
                     VQGYGMTETASLISLNHPFSAREGSVGKILPGREFKLAEDGEILVRGESVSSGYWENG
                     ELREGADGGWLRTGDLGELDANGNLKFRGRKKNVIVTAAGLNIYPEDLEEALKKQPAI
                     RDAVVVAIEKGGNADACAVLLKNTGVADDGAWSAVAAANASLAEYQQIRNWKVWPDPD
                     FPRTPTGKPRLAVMAEWAAGGKIDGNDEAARKASAARSDPITRFLSEGSLNSLSSLDR
                     VELLSVLEQRYNVEINEADFSQAASAVELRRLVEKAGAEEFEGSGSRASRYQYPRWAQ
                     FEPIRWLRLLVYYLLVWPATQILAHPR"
     gene            complement(2058..3857)
                     /locus_tag="VFR42_10275"
     CDS             complement(2058..3857)
                     /locus_tag="VFR42_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369588.1"
                     /translation="MNSAGTGNEVSAVYLARLTAQRAQQEAAERRHKQLGYLKLVVVA
                     ATVLVFVLGLKAQIASAWWLIVPIVALAFLEKAHERVLGSLKKCTRVMAFYERGLARM
                     EDRWMGGGETGGDFLEPEHPYARDLDLFGEGSLFELLCTARTSTGKNILAEWLMGAAA
                     VSEIERRQEAVRELAPRLDFREEVAASGEDIRSRARTEALLAWAEAPPVLHPKAVRVV
                     ALILSAGWLLSLLVWAVWGLWEVTLVISIANRIFYSHFQSRCQKIIGAETFVREFALL
                     PILLTRMEQENFASAKLVELQTRLRQHGVSPAASIGKLRRLTQALESRRSLLIAVSDS
                     FVLWTTQVAFAIETWRQEFAPAFRVWLMAVGEMEALNALANYCYERPEDVFPQFLTEP
                     AATLEARGLAHPLLPRSRAIRNDVKLGAELRVMIVSGPNMSGKSTFIRAIGLNGVLAQ
                     CGAPVCAKRLQLSPLQVTASICILDSLQGGVSRFYAEIKRLKLVAELAEKPSAVLFLL
                     DELLSGTNSDDRRAGSEAFVKSMVARGAIGLVTTHDLALTRMGDELPMHAANFHFEDR
                     IENGELRFDYRLVAGVAQSSNALKLMRSIGLEV"
BASE COUNT          853 a         1408 c          948 g          820 t
ORIGIN      
        1 ccgcggatgt gccagaattt gcgtcgccgg ccataccagg aggtagtaca cgagcagccg
       61 caaccagcga atcggttcaa attgtgccca tcgcggatac tgatagcggc tggcccgcga
      121 accactgcct tcgaattctt ctgcgcccgc cttttcgaca agcctgcgca actcgaccgc
      181 cgacgcggct tgcgaaaaat ctgcctcgtt gatttcgacg ttgtagcgct gctccaacac
      241 ggacagcaat tccacacgat ccagggaaga cagcgagttc agtgatcctt cagagagaaa
      301 tctggtgatt ggatctgatc gcgcggcgct ggcctttcgc gcggcttcgt cgtttccatc
      361 aatctttcca cctgccgccc actcggccat cacagccaat cgcggctttc cagttggcgt
      421 ccgcggaaag tccggatccg gccacacttt ccagtttcgt atttgctggt actccgcgag
      481 cgaagcattt gcggcggcca cagccgacca tgcgccatcg tcggccaccc cggtgttttt
      541 tagcaacacc gcacaggcat ccgcattccc acccttctca atcgcaacca ccaccgcgtc
      601 ccggatcgcc ggctgctttt tcagtgcttc ttccagatct tcgggatata tattcaatcc
      661 cgccgccgtc acaatcacat ttttcttgcg cccgcgaaat ttcagatttc cgttcgcgtc
      721 cagttcacct aaatcgcccg tgcgcagcca gcccccatct gctccctcgc gcagctcccc
      781 gttttcccaa taacccgaag aaacactctc cccccgcacc agaatttcgc cgtcttccgc
      841 caatttgaat tctcgccctg gcaggatttt tcctaccgag ccttccctcg cgctgaacgg
      901 atggttcagg ctgatcagcg aagccgtctc cgtcatcccg tagccctgca ccacggcaaa
      961 gccaaggcgt ttgatgaaca attccgttgt cgctcccaac gcagcgccac cggaaataaa
     1021 tgcccaaaac cggaatccca gcagccggtg aattcgccga aacctccagg cccttcgcaa
     1081 aaacgattcg cccgacgcag cttcgtagtt cctgtcaaac cattttcgcc agcctcgcgc
     1141 gtcgatttcg cgttccatct gcactcgaac tagatccagc atgcgtggca ccgcaatcaa
     1201 tagcgtcgcc ttttcccgct tgagcgttcg cacaatgtcc gctggattcg cggaattctc
     1261 aaacaccacc ttcgctccaa acagcggcgg cacaaacaat cccatgaact gcccaaacac
     1321 atggctcagc gggaccagcg aaacaaaccg gagaggatgc agccactttt cgtatttcag
     1381 atacggcagc atcgcgcgct caatcggctc cagattcgcc agaaaatttc cgtgcgtcag
     1441 caccactccg cgcggttccg ccgtcgtccc cgacgtaaaa aggatttccg caatcgcttc
     1501 gcgccctact gagcagtccc ctgcgtctga gcgccgctct cccgatcggc gcgcttcaat
     1561 cgaacgccgt tccgacaacc acagcgtcct gacctttcca gaaatctcct ctcctctaga
     1621 ttcccgtaaa atcagtttcg cccccgactg cgccaccgtc cgccgcacaa aatccagact
     1681 cgccccggca tccatgggta ccgccaccgc tccgcgtcgc agcactcccc aaaacgccga
     1741 cacccacccc gcagagttcg ctccccacaa gagcactcgg tcgcccgcac caatcccttc
     1801 cttttccagc accgcactcc aattctccgc tgccgcgtgc agctccgcat atgtcagcaa
     1861 atgccggcgg tacccgcgcg tttccacgac agccccgtcg ttacggaatc ttaagaagga
     1921 atcggccagc gaattcagat tggtgcgtgt catctgcttt ttcgagccaa tcttaactct
     1981 tcgggaggac ggcgaagaac ctcggcaagt ttctattcat catgggggcc tctcgcacgt
     2041 ggcgtgccag cccgggccta cacttccagc ccgatcgacc gcatcagctt cagcgcgttg
     2101 ctgctctgtg ccacgcccgc caccagccgg tagtcaaatc gcagctcgcc attctcaatg
     2161 cggtcttcaa agtgaaaatt cgccgcatgc atcggcaact catcccccat tcgcgtcagc
     2221 gccagatcgt gcgtagtcac aagcccaatc gctcctcgcg caaccatgct tttcacaaag
     2281 gcctccgacc cggcgcgccg gtcgtcggag ttcgtcccgc tgagcagttc gtccagcaga
     2341 aacaacaccg ccgaaggttt ttccgcaagt tccgcgacta gcttcaagcg tttgatctcg
     2401 gcgtaaaaac gcgacacgcc cccttgcagc gaatccagaa tgcagatcga agccgtcacc
     2461 tgcagcggcg atagctgcaa tcgcttcgcg caaacaggag ccccacactg cgccaacacg
     2521 ccattcagac ctatcgcacg gataaacgtg ctcttgcccg acatgttcgg gccgctgacg
     2581 atcatcactc gcagctccgc gccaagtttg acgtcattgc ggatcgcgcg cgagcgcggt
     2641 agcagcggat gcgccagtcc gcgcgcttct aacgttgctg ctggctctgt taagaattgt
     2701 gggaatacat cttccggccg ttcgtaacaa taattcgcca gcgcattcag cgcttccatt
     2761 tcgcccacgg ccatcagcca cacgcgaaaa gccggtgcaa attcctgtct ccaggtctcg
     2821 atcgcaaaag caacttgcgt cgtccacagc acaaatgagt cgctgaccgc gatcaaaagg
     2881 ctgcgacgtg attccaacgc ctgcgtcagt ctcctgagtt ttccaatgct cgctgccggc
     2941 gacacgccgt gctgtctgag ccgcgtttgc agctcgacca gctttgcgga agcgaaattc
     3001 tcctgttcca tgcgcgtgag aagaattggc aagagcgcga actcgcggac aaacgtttcc
     3061 gcgccgataa ttttttggca gcgtgactgg aaatgcgagt aaaagatacg attcgcaata
     3121 ctgatcacca gcgtaacttc ccaaagtccc cacaccgccc ataccagcaa gctcaatagc
     3181 cagcccgctg acagaatcag cgcaaccact cgcaccgcct tgggatgcaa caccggaggc
     3241 gcctcggccc acgccagtag cgcttcggtc cgcgcacgcg accggatgtc ctcgccggac
     3301 gcggcgacct cttcccgaaa atccagccgc ggcgccagtt cccggacggc ctcctgccgc
     3361 ctctcgatct ctgacaccgc cgcggcgccc atcagccatt ccgccagtat gtttttgccc
     3421 gtcgaagtgc gcgccgtgca cagcaattcg aacagcgatc cctcgccgaa caaatccaag
     3481 tcccgcgcat agggatgctc cggctccaga aaatcgccac ccgtttcccc gcctcccatc
     3541 cagcggtctt ccatccgggc cagaccgcgc tcataaaaag ccatgacccg cgtacatttc
     3601 ttcagcgacc ccagcacgcg ctcgtgcgct ttttctagga acgccaacgc cacgatggga
     3661 actatcagcc accaagccga cgcgatctgc gccttcaacc ccagcacaaa taccaagacc
     3721 gttgctgcca cgacgactag cttcaaatat ccgagttgtt tgtggcggcg ttccgcggct
     3781 tcttgttggg ctctttgcgc agtcaggcgg gccaaataca cggcagagac ttcgtttccc
     3841 gtcccggcgg agttcatccc gcaacactat atagcaactg ccctctcgag cacgacaccg
     3901 ccatcgcgtc aaatttttcg aaagaatcaa gcagagagat ctggttaatg cgcgaccctt
     3961 gccaacggcg cgtttctgat tatccagcct aagtgtgcat gcgacatgta tccaatatgt
     4021 tgatagcaa
//
LOCUS       DASTPM010000582         4085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_366765, whole genome shotgun sequence.
ACCESSION   DASTPM010000582 DASTPM010000000
VERSION     DASTPM010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366765"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VFR42_10280"
     CDS             complement(<1..229)
                     /locus_tag="VFR42_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369589.1"
                     /translation="MNTTENSSANTTRRTFLKVASAGIALNSTAVSYAKILGANDRVR
                     VGLCGFSERFRDALLPAFHQHAAELNFEFAGV"
     gene            complement(226..1200)
                     /locus_tag="VFR42_10285"
     CDS             complement(226..1200)
                     /locus_tag="VFR42_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0369590.1"
                     /translation="MNRREWMQASGAAAVSALSAPYTRVFSRPEAKLDCVFQLSVITD
                     EISQDFGHAVEVAAKEFGVGMVELRGLWNKNIVNLDAKEVAEVQSLLKKYDLKVTDIA
                     SPLFKVDWPGAPKSKYSPKGDAYNASFSFDQQDEVLERAANMAHAFGTNRIRLFDFWR
                     LEDDKPFRKDMDEKLSEISAKAAKQSMILILENEYECNTATGPESARTLGAVPNKNFF
                     LNWDPGNAARLGDKAFPEGYALLPKDRIGHMHLKDVKRNADGTYEWECMGRGIIDYAE
                     QFRVMMRDGYRGTMSLETHWKGAGTMEESSRQAMAGTKELLRKAGALK"
     gene            1373..2515
                     /gene="serC"
                     /locus_tag="VFR42_10290"
     CDS             1373..2515
                     /gene="serC"
                     /locus_tag="VFR42_10290"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEU0369591.1"
                     /translation="MSSTTTAALHRVHNFNAGPSALPLSVLDRIREELLDWCGSGMSV
                     MEMSHRSPEFESINAAAEAGLRKHLAIPDDYAVIFLQGGGSTQFTLVPMNLCLPGKPV
                     DILHTGTWTAKALAELKKGIAHRVAANTEAVKHTRVPGKEEITLGADASYVYLCTNNT
                     IEGTQYHSLPATGDVPLVADMSSDIASRPLDVSKFGLIFAGAQKNLGPSGATIVIIRK
                     DLAERADKNLPTIFQYRTHIKEKSLYHTPPTFAVYVVGLMMEWIESQGGLNGIQKRNE
                     TKARLLYDAIDSSNGFYHCPVEKDSRSLMNVVFRVAGTRGKSAATHPDETAEKEFGKQ
                     AAAAGLTGTPGHRSVGGMRVSLYNAVEPSSVETLVGFMREFQRKRG"
     gene            2633..3178
                     /locus_tag="VFR42_10295"
     CDS             2633..3178
                     /locus_tag="VFR42_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369592.1"
                     /translation="MTVYTVVLYSHLLGALALFIGYGIEWVSSSLFRSAASTEQVRSW
                     LRVFQVSPPLSGAGLGVLLLSGGYLASLSGAMKQGWIPATLLAIGIALLLGFVLILPR
                     MRKIRTALPSLNEPVSAALRARLSDPILLSAIRIRVLLAAGIVYLMAAKIPFTPSLIA
                     LAVALGAGFLFAIPVWKRVSA"
     gene            complement(3300..3998)
                     /locus_tag="VFR42_10300"
     CDS             complement(3300..3998)
                     /locus_tag="VFR42_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU0369593.1"
                     /translation="MISIRKSDERGHANHGWLDTRFTFSFADYYDPKHVQFRTLRVMN
                     DDRVAGGGGFPTHPHRDMEIVTYVLEGALAHKDSMGNGSTIKPGDVQYMSAGTGVAHS
                     EFNASKTEPVHLYQIWMLPDKNGHKPTYDQKNFSEGEKRGRLRLVVSPDGRDGSVKIR
                     QDNELYATVLGAGESVKHELRPERHAYVQVARGSVTLNGKPLEVGDGAEVSAEKTLEL
                     TGVKDAEVLLFDLV"
BASE COUNT          752 a         1318 c         1084 g          931 t
ORIGIN      
        1 ttacgccggc aaattcaaaa ttcagctcgg cagcgtgctg gtgaaaggct ggaagcagcg
       61 cgtccctgaa ccgctctgag aatccgcaca agccaacgcg cactcggtca ttcgcgccaa
      121 ggattttggc gtaactgacc gccgtggaat tcaaggcgat ccctgcactc gctactttca
      181 aaaacgttcg ccgagtcgtg tttgcggaac tattttcggt ggtattcatt tcagtgctcc
      241 cgctttgcgc aacaattcct tcgtgcccgc catggcctgc cgcgaagact cttccatcgt
      301 gcccgcgcct ttccagtgcg tttcgaggct catcgtaccg cgataaccgt cacgcatcat
      361 gacgcggaat tgctcggcgt agtcgatgat gccccggccc atgcattccc actcgtaagt
      421 cccgtcggcg tttcgcttga cgtcctttag gtgcatgtga ccaatgcggt ccttgggcaa
      481 aagcgcgtag ccttcgggaa acgccttgtc acccagccgc gccgcgttgc cgggatccca
      541 gttcaaaaag aaattcttgt tcggcaccgc gcccagcgta cgcgccgact ccggcccggt
      601 cgctgtgttg cattcgtatt cgttttcgag aatcagaatc atgctctgct tggcggcttt
      661 ggcggaaatc tcgctcagct tttcgtccat gtccttgcga aaaggcttgt catcttccag
      721 tcgccagaag tcgaatagtc ggatccggtt agtgccgaac gcgtgcgcca tgttggcagc
      781 gcgttccagc acttcatcct gctggtcgaa cgaaaagctc gcattgtacg catcgccctt
      841 cgggctgtac ttcgacttcg gtgcccccgg ccagtccacc ttgaacagcg ggctggcgat
      901 gtcggtcacc ttcaggtcat atttcttgag caggctttgt acttccgcaa cttctttggc
      961 atccaggtta acgatgttct tgttccacaa gccgcgcagt tcgaccattc cgacgccgaa
     1021 ctctttcgcg gcaacttcga cggcgtggcc aaagtcctgc gaaatttcgt cggtgattac
     1081 ggaaagctga aacacgcagt ccaacttggc ctccggccgg gaaaataccc gcgtataagg
     1141 agcgctcaac gcgctcactg ctgccgctcc gcttgcctgc atccattctc tgcgattcat
     1201 ggccgaattt ctcccggtgg gattctactc ctgctgattc gggaaggtta gcgcaatcca
     1261 atgagccgcg cggaccgcca tgttattctg aagggctggc ggcatgggtg cggtccacgc
     1321 aggacgcggc acttcaattc gtaaccggcc gtcacagaga ggatctttga tcgtgtcgag
     1381 caccaccact gctgcgctcc atcgtgtcca caatttcaac gccggcccca gcgccctgcc
     1441 gctttccgtc ctcgatcgca ttcgcgaaga gctgctcgac tggtgcggct ccggcatgtc
     1501 cgtgatggag atgagccatc gctcgccgga atttgaaagc atcaacgccg cggccgaagc
     1561 cggcctgcgc aagcacctgg cgattcccga cgactatgcg gtgatttttc tgcagggtgg
     1621 tggcagcacg caattcactt tggtgccgat gaatctttgc ctgcccggca agccggtgga
     1681 cattctgcat acgggaacgt ggacggcgaa agcgctcgcc gagttgaaga agggcatcgc
     1741 gcatcgcgtg gcggccaaca ccgaagcagt aaagcacaca cgcgtccccg gcaaagagga
     1801 aatcaccctg ggcgccgacg cgtcttatgt ctacctctgc accaacaaca ccatcgaggg
     1861 cacgcaatat cattcgttgc ccgctaccgg cgatgttccg ctcgtcgcgg acatgtcctc
     1921 ggacatcgca tcgcgcccgc tggacgtcag caaatttgga ctcatcttcg ccggcgcgca
     1981 gaaaaatctt ggcccttccg gagcgacgat cgtgatcatc cgcaaggacc tcgccgaacg
     2041 agccgacaaa aatctgccca ccatttttca gtaccgcacc cacatcaagg aaaaatccct
     2101 ctaccacacg ccgccgacat tcgccgtcta tgtcgtgggt ttgatgatgg aatggattga
     2161 atcgcagggc gggcttaacg gaattcaaaa gcgcaacgaa accaaagcca ggttgctgta
     2221 cgacgccatt gattccagca acggcttcta ccattgcccg gtggaaaagg attcgcgctc
     2281 tctaatgaac gtggtcttcc gcgtcgccgg caccagggga aaatccgccg ccacgcatcc
     2341 cgacgaaacc gctgaaaaag aatttggcaa acaagccgcg gctgccggtc tcaccggtac
     2401 tcccggtcac cgttcggtcg gcggcatgcg cgtttcgctc tacaatgcgg ttgagccatc
     2461 ctccgtcgag acgctggtcg gcttcatgcg cgaatttcag cgcaaacgcg gctaagccgt
     2521 ccgtttcggc ggagatccat cctcatctgg agagcgcgcc cgcagggcgg taacaatcat
     2581 cttccagaag gttatttgct gagctgggac ctgtcttgtc gatgaggtac aaatgaccgt
     2641 ctacacagta gtcctatatt cacacttgct cggcgcgctt gccctcttca tcggctacgg
     2701 catcgaatgg gtgtcgtctt cgttattccg cagcgccgcg tccacagaac aagtgcgctc
     2761 ctggctgcgc gtcttccaag tttcgccgcc gctttccgga gccggcctcg gcgtgctgct
     2821 tctttccggc ggctaccttg cgtcactctc cggagctatg aagcagggct ggattcccgc
     2881 cacgcttctc gccatcggta tcgcccttct cctcggtttc gtgctcatcc tgccgcgcat
     2941 gcgcaaaatc cgcaccgcgc ttccttctct gaacgagccg gtctcagccg cgctgcgggc
     3001 tcgactctct gatccgattc tactctccgc gatccgcatc cgtgtccttc tggcggccgg
     3061 catcgtctac ctcatggctg caaaaatacc gttcacgcct tcgctgatcg ctctcgccgt
     3121 agcgcttggt gccggttttc tttttgcgat cccggtttgg aaacgcgttt cggcctgacg
     3181 ccggtagtcc ttcgcccggc gcgactcttt gcgcccacaa aataacacca caaagagagg
     3241 aaccggcttc cccggcgaaa gcggtccctc accatccttc ttttttttcg gttacgctgt
     3301 tagaccaaat cgaacagcag cacttcggcg tcttttacgc cggtcagttc cagcgttttc
     3361 tccgcggaaa cttccgcgcc gtcgccgact tccagcggct tgccattcag cgttacgctg
     3421 ccgcgcgcta cttgcacgta cgcgtggcgc tccggcctca gttcatgctt cacgctctcg
     3481 cccgcgccca gcaccgtcgc gtacagctcg ttgtcctggc ggatcttcac cgagccgtcg
     3541 cgcccgtccg gcgaaaccac cagccgcagc cggccgcgct tttccccttc ggaaaaattc
     3601 ttctggtcat acgtgggctt gtggccgttc ttatccggaa gcatccagat ctgatacagg
     3661 tgcaccggct ccgtcttcga agcgttgaac tccgaatgcg ccacgcccgt tcccgcgctc
     3721 atgtactgca catcacccgg cttgatggtc gagccgttgc ccatgctgtc cttgtgtgcc
     3781 agcgcgcctt ccagcacgta cgtcacgatt tccatgtcgc ggtgcgggtg cgtgggaaat
     3841 cctccgccgc cggcgacgcg atcgtcgttc attacccgca acgtgcggaa ttgcacgtgt
     3901 ttcgggtcgt agtaatccgc gaaggaaaac gtaaaccgcg tatccagcca gccgtggttg
     3961 gcgtgcccgc gctcgtcgct cttgcgaatg ctgatcatgg tgcctcctca aggccatccc
     4021 tcatttccga ggccattata ttcgttataa cgactattgt caagaggtgt atctgtgcgt
     4081 cgcct
//
LOCUS       DASTPM010000583         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_424553, whole genome shotgun sequence.
ACCESSION   DASTPM010000583 DASTPM010000000
VERSION     DASTPM010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424553"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..>2715
                     /locus_tag="VFR42_10305"
     CDS             244..>2715
                     /locus_tag="VFR42_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU0369594.1"
                     /translation="MRIRIQKGFWTSKYGLTLLGVVFGCVVVAASVFGYYYVKYGRMI
                     DARLSGNILQNTTQIFSAPEEISPGQAWGPDDLTTYLQRVGYRPEADDAALGQYTANG
                     NVVDIRPSKLSYFSGSNALAVQFAGKAIRSIRPLSGGSEMAVAEIEPELITNLFDSAR
                     EKRRPVRYDDLPPTLINAILSAEDKRFFEHPGFDFIRIFGAAWNDLRHGTHLQGASTI
                     TMQVARTFFLSTDRNLRRKLAEAMLSFELEQRFSKQKIFEMYANEVYLGNRGSFGIRG
                     FSQASVAYFGKDLRQLSVGECAFLAGLIRAPNYYSSADRHPERGAQSRDRVLTQMVEN
                     KYITSADAQDAKTAPLKLVRTATTSSEAPYFVDMVKDHLLDKYSENDLLSQNYRVYTT
                     LDPALQRAAAAAVEAGMKNVDDLLAKKYAKKKTAKGKTTGTKEILPQVQVALVAMDPR
                     TGEIRALVGGRDYGQSQLNHAIAHRQPGSVFKPFVYAAAFDNAVDGVQPVVTPATTID
                     DEPTTFEFDGQEYTPNNYGEKFMGRVTVREALTNSLNVATVKVAELIGYGRVVQIARQ
                     MGLGNNIRATPAVALGAYEMTPVDVAAGYTAFGQNGVRAEPQFLHEMVSANGAILEKT
                     SPETHSVLDPRVAYLVTSLLKDVLNKGTGATVRARGFSLPAAGKTGTSRDGWFSGFTS
                     NLVTVIWIGFDDNRDLGFAGGQTAAPIWADFMKKATTLPGYRNVKDFDLPEGVQSVMI
                     DPESLELATSGCPTIREEVYVGGSAPTQYCELHGGGHGIISSAGSVLSHIFGGGQPTA
                     PVDPAKPGGAGAPGETKTVIGPDGKP"
BASE COUNT          583 a          697 c          928 g          507 t
ORIGIN      
        1 ggttttcggc cccgtcttct gtcagagaag aataagggcg ggccagcgac cgcaccgcag
       61 ggttcaggcg gccgcatcct tcgtaagtcg agaccaagca aaggcacgcg cgatgggacg
      121 catctaaaca aatcggaaac agatgaggaa ctgtggcttt gctaagcggc tgcagtacga
      181 gacactaaat gcgtgggcta gcgtccaaat gcttagcccg cctttcagcc cgttggagaa
      241 aacttgagaa tccgcataca aaagggattc tggacgtcga agtacggcct gaccctgctg
      301 ggtgtggttt ttggctgtgt ggtggttgcg gcaagcgttt tcggctacta ctacgtgaaa
      361 tacgggcgga tgattgacgc ccggctatcc ggcaacatcc ttcagaacac aacacagatt
      421 ttttcggcgc ccgaagaaat ttcgcccggg caggcctggg ggccggatga cctgaccacc
      481 tacctgcaac gggtgggcta ccgcccggaa gcggacgacg cggccctggg gcagtacacc
      541 gcgaacggga atgttgtaga tatacggcct tcgaaactgt cgtatttcag cggcagcaac
      601 gcgctggcag tacagtttgc cgggaaagcg attcgctcga ttcgccctct gagtggcggg
      661 tcggagatgg cggtcgcgga aatcgagccg gagctaatca ccaacctttt tgacagtgct
      721 agagagaagc ggcggccggt gcggtatgac gatctgccgc cgacactgat caacgcgatc
      781 ttgtcggcgg aggacaagcg ctttttcgag catcccgggt tcgattttat tcgcatcttc
      841 ggcgcggcat ggaacgactt gcggcacggt acccatttgc agggtgccag caccatcacc
      901 atgcaggtgg cgcggacgtt cttcctgagc acggatagaa atttgcggcg caagctggcg
      961 gaagccatgc tgagcttcga actggagcag cgcttttcga agcagaagat ttttgagatg
     1021 tacgcgaacg aagtgtacct ggggaaccgg ggcagctttg gcattcgcgg gttttcgcag
     1081 gccagcgtgg cgtacttcgg caaagacttg cggcagctct ctgtggggga gtgcgcattc
     1141 ctagccggac ttatccgtgc tccgaattac tactcgtcgg cggaccggca tccggagcgt
     1201 ggcgcgcagt cacgcgatcg tgtgctgacc cagatggtgg agaacaaata catcacgtca
     1261 gcggatgcgc aagatgcgaa gaccgcgccg ctgaagctgg tgcggacggc gacgacgtcc
     1321 agcgaagcgc cgtacttcgt ggacatggtg aaagaccacc tgctggacaa atattccgaa
     1381 aatgacctgt tgagccagaa ctatcgcgtg tatacgacgc tggacccggc tttgcagcgg
     1441 gctgccgcag ctgcggtgga agccggcatg aagaacgtgg atgacttgct ggcaaaaaaa
     1501 tacgccaaga aaaagacggc aaagggaaag accacgggca ccaaggaaat attgccgcaa
     1561 gtgcaggttg cgctggtggc catggacccg aggacgggag agattcgcgc gttggtgggc
     1621 ggtcgtgact atgggcagag ccagttgaac catgcaattg cgcaccggca gccgggatca
     1681 gtgttcaagc catttgtgta tgcggcggca ttcgataacg cggtggacgg agtgcagccg
     1741 gtggtgactc cggcgacgac gatcgatgac gaaccgacga cgtttgagtt cgacgggcag
     1801 gagtacacgc cgaacaatta cggcgaaaag ttcatgggcc gggtgacggt gcgtgaagcg
     1861 ctcaccaatt cgctgaacgt ggccaccgta aaagtggcgg agctgatcgg gtacgggcgc
     1921 gtggtgcaaa ttgcgcggca gatgggactg ggcaacaaca ttcgcgcgac accggcggtg
     1981 gcgttgggcg catacgaaat gacgccggtg gatgtggcgg caggctatac ggcgtttggc
     2041 caaaatggcg tgcgagccga accgcagttc ctgcacgaga tggtgagtgc gaacggcgcg
     2101 attctggaga aaacatcgcc ggagacgcac agcgtgctgg acccgcgagt ggcgtacctg
     2161 gtgaccagct tgttgaagga tgtgttgaac aagggcacgg gcgcgacggt gcgtgcgcgg
     2221 gggtttagtt tgcccgccgc ggggaaaacg ggaacttcgc gggacggctg gttttccgga
     2281 ttcacgtcga acctggtgac ggtgatctgg atcggatttg atgacaatcg cgacctggga
     2341 ttcgccggcg gccagacggc ggcgccgatc tgggcggact tcatgaagaa ggcgacgacg
     2401 ctgccgggct atcgcaacgt gaaggatttt gacttacccg aaggcgtgca gtcggtgatg
     2461 attgacccgg agtcgctgga gctggcgact tcgggctgtc cgactatccg ggaagaagtg
     2521 tacgtaggcg ggtcggcgcc gacgcagtat tgcgagttgc acggcggggg ccatgggatc
     2581 atttcgtcgg caggttcggt gttgtcgcac atttttggcg gtggccagcc gacagcgccg
     2641 gttgatcctg caaaaccggg cggtgccgga gcgccgggcg agaccaaaac ggtgatcggt
     2701 ccggacggca aaccg
//
LOCUS       DASTPM010000584         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1174403, whole genome shotgun sequence.
ACCESSION   DASTPM010000584 DASTPM010000000
VERSION     DASTPM010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174403"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1112
                     /locus_tag="VFR42_10310"
     CDS             120..1112
                     /locus_tag="VFR42_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369595.1"
                     /translation="MVRINQRIARIAICFAAAISASAGLALADKPSKSSPSSCFSADS
                     RDKSLREVPCDLSTIQVAARSGEVYAQNQLGLVAALALENGRDIRDARSWFEKAARRG
                     YAPAQVNLAVTYINGWGTPQNYGAALNWLTAAAKQNHPRAYANLGILYMNGLGVRRDY
                     AEALKYLQLAANAGDAGAQANLGYLYDGGLAVVQDYSAAARWYRLSAEQGNALGQNNL
                     ADLYLRGLGVHQSYNDAFFWFQKSAQQGYTAASIKLGFLLMNGLGTAKDPATAYSWLL
                     SASQAGDHRGDEYLHTLESLLSQRQLADARTRSESRPASFVRVTPQIVASSLVQ"
     gene            1222..1599
                     /locus_tag="VFR42_10315"
     CDS             1222..1599
                     /locus_tag="VFR42_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369596.1"
                     /translation="MKFAKIVFRIAAIWGFLIITPLYFLFDLVGKNDPPPITHPAFYY
                     GFVGVALVWQFAFLIIASDPIRFRLLMIAAILEKLVYSVPVIILVSQKRMNPNDLVFA
                     GIDLFLGVLFVFAYLRTPRAPAA"
     gene            complement(1648..2358)
                     /locus_tag="VFR42_10320"
     CDS             complement(1648..2358)
                     /locus_tag="VFR42_10320"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0369597.1"
                     /translation="MRMIGRVRGWRSRRVAGRMLGSATLGTLVFAAVALGLTGKTVGA
                     ERKSRALFNGRNLDGWGHVGPGRFLVRNGLLETEGGMGLLWYTREKIGHARLRVVFKM
                     EGKEPDSGVFIRIPEKPTEPWMPVNRGYEVEIGDWPSDYSCTGVLYTFTKALARPLKP
                     LGEWNTMDITIDGALTIVFLNNVKVTDFTEGQPVPPKPEGSGDPDRGPRPETGYIGLQ
                     NHPGGKVYFREVSLTPLD"
BASE COUNT          474 a          924 c          620 g          575 t
ORIGIN      
        1 atccaccgtg tattgaggta gccccgtccc tccgccagtc tctgccccat agacgctgtg
       61 cccggcccca aagcctcacc tctcgcttca ggggcgggca cagcccgaaa ggggactcca
      121 tggttagaat caatcaacgc atcgcccgca tcgcaatttg cttcgcagcc gccatctcgg
      181 cttctgcagg tttggctctc gccgacaaac cgtccaagtc atctcccagc tcgtgcttca
      241 gcgccgacag ccgcgacaag tccttgcgcg aagtaccctg cgacctctcc acaatccagg
      301 tcgcggctcg ctccggcgaa gtctacgccc aaaaccagct cggcctggtc gcagctctcg
      361 cgcttgaaaa cggtcgtgac atccgcgatg cccgctcctg gttcgagaag gctgctcgcc
      421 gcggttacgc tcccgcccaa gtgaatctcg ccgtcaccta catcaatggc tggggcactc
      481 cccagaatta cggcgccgcg ctcaactggc tcaccgccgc tgccaaacaa aatcatccgc
      541 gcgcctacgc caacctcggc atcctctaca tgaacggttt gggagtccgc cgcgactacg
      601 ccgaagccct taaataccta caactcgccg cgaatgccgg tgacgccggc gctcaggcca
      661 atctcggcta cctatacgac ggcggcctcg ctgtcgtgca ggactattct gctgctgcgc
      721 gctggtatcg cctttccgcc gaacagggca atgccctcgg ccaaaacaat ctcgccgatc
      781 tctacctccg tggtctcggc gttcaccaga gttacaacga cgctttcttc tggttccaga
      841 aatccgcgca gcagggctac actgcggcca gcatcaagct cggcttcctc ctgatgaacg
      901 gcctaggaac agctaaagat cccgcgaccg cctattcctg gctgctctcc gcatcccaag
      961 caggtgacca tcgcggcgat gaatatctcc acacgctcga gtcactgctc agccagcgcc
     1021 agctcgccga cgcccgcacg cgttctgaat ccagaccggc ctcattcgta cgcgtcaccc
     1081 cgcaaatcgt cgcctcgtcg ctcgttcaat agaattcgtg ccgacagctt aacgatcagc
     1141 cggtcgcctg ggggccgctg cccacggccc ctggctccct tctccgtgcg aacgcgacgt
     1201 gcattgcgct aaaatcgcgg catgaaattc gcaaaaatcg ttttccgcat cgccgccatc
     1261 tggggttttc tgattattac gccgctgtac ttcctgttcg atctcgtcgg caaaaatgat
     1321 ccgcctccca tcactcaccc agcgttttat tacggctttg tcggagtggc gctcgtttgg
     1381 cagttcgctt tcctgatcat cgccagcgac cccatccgtt ttcgcctgct gatgatcgcg
     1441 gccatcctcg aaaaactcgt gtactccgtt cccgtcatca tcctggtctc gcaaaaacgc
     1501 atgaatccca atgatctcgt cttcgccggc attgatcttt tccttggcgt ccttttcgtc
     1561 ttcgcttatc tccgcacgcc tcgcgctcct gccgcgtagc tcttcgctcg ttcgcgcgtg
     1621 ccggtcttcc ttttaggcgg aatagcttca atccaatgga gtcaacgaaa cttctcgaaa
     1681 ataaactttc ccgcccggat ggttctgcaa tccgatgtaa cctgtctctg gcctcggccc
     1741 gcgatccgga tcgccggatc cctcaggctt cgggggcacc ggctggccct ccgtgaaatc
     1801 cgtcaccttc acgttgttca gaaacacgat cgtgagcgcg ccatcgattg tgatgtccat
     1861 ggtgttccat tcgcccagcg gttttagtgg ccgcgccagc gcctttgtga atgtgtacag
     1921 cacgcccgtg cacgaataat cgctcggcca gtctccgatc tccacctcgt aaccgcggtt
     1981 caccggcatc cagggctccg tcggcttttc cggaatgcga atgaacacgc ccgaatccgg
     2041 ctctttgcct tccatcttga acaccacacg tagcctggcg tggccgatct tctctcgcgt
     2101 gtaccaaagc aatcccatgc cgccttccgt ctccagaagt ccgttgcgca ccaggaatct
     2161 tccaggccct acgtgccccc atccatccag gttccttccg ttgaagaggg ctcggctctt
     2221 ccgttctgca ccaacagtct ttcccgttag accgagcgcg acggccgcga acaccagcgt
     2281 acccaacgta gcggagccca acatccgacc cgctactctt ctcgacctcc agccccgaac
     2341 ccgaccgatc atgcgcattc gatttgactc cgcctccctt gcggtgtaag aaatcttcgg
     2401 gatctcattt gctccacttt cagtatcgcc gtaaaaggac tttcacgatg gccggacaag
     2461 gcatcgatcg tcgccagatt ctgcgctaca tcagcatcgc atctgtcgcg tcaacattcc
     2521 cgggctttcg ccagtggacg tttgcctgtt cgcacgacga gcatgccgct gctgccgccc
     2581 tcacgcaagc tcc
//
LOCUS       DASTPM010000585         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_251321, whole genome shotgun sequence.
ACCESSION   DASTPM010000585 DASTPM010000000
VERSION     DASTPM010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251321"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2379
                     /locus_tag="VFR42_10325"
     CDS             <1..2379
                     /locus_tag="VFR42_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0369598.1"
                     /translation="EGAKLAFGRYINTVYRVEKAEVILSLDSDFLASGSGHVRYMKDF
                     YRRRKLDEFAEVDRAGGEMNRLYVVEPTPSVTGSSADHRLPLRFVEVEQFARALAAKL
                     GLGGSGSLSAAAEKWLDIVVKDLQKNHGRSLVVAGEQQPAEVHALVHAINATLGNIGN
                     SVYYTEPVEANPVNNIESLRDLCADIDAGRVSTLLIVGGNPVYDAPHDFDFTSKLRKV
                     AHTIHLSSHFNETSEHCKWHLAESHYLESWGDARCFDGTVSVIQPLIAPLYHTHSASE
                     VLAAFSDKPGLSGYDAVRNRLKAANPNGDFEKFWRKTLNDGVVANSTYPALSLTPKFN
                     AASLPAIPPGNPGELEFLFRPDPTIHDGRFANNGWLQELPKPVTKLVWDNAALVGPNT
                     SWNLGITHTVAARGGEHGQIVSTVVDITLNNSKVTAAGWILPGQAEGVVVLPLGYGRK
                     KAGYTGTNKGFNAYAVRTSNALWRATAGSESVKKTGEEYSLGCTQYHFNMEGRKILST
                     GTLEEYKKNPGFAHEGDETPPKSLSLYEEFKYPGYAWGMAIDLNNCNGCNACVVACVA
                     ENNIPVVGKDQVLRGREMHWIRIDRYYNANTPTGDPRTNENNALANPETFFQPVPCMQ
                     CENAPCEQVCPVGATVHSAEGLNDMVYNRCIGTRYCSNNCPYKVRRFNFLRFQDWETP
                     QLKLQRNPEVTVRSRGVMEKCTYCVQRINNARIEAEKQNRPIADGEIVTACQASCPSE
                     AIVFGNANDPNTRVAKLKAQQRNYNVLGELNSRPRTTYLGAVRNPNPDLEKA"
BASE COUNT          523 a          782 c          644 g          470 t
ORIGIN      
        1 gaaggtgcca agctggcttt cggtcgttac ataaacaccg tgtatcgcgt ggaaaaggcg
       61 gaagtcattc tgtcgctcga ttcggacttc ctcgccagcg gctctggtca tgtccgctac
      121 atgaaagatt tctatcgtcg gcgcaagctc gacgaattcg ccgaggtcga ccgcgcgggc
      181 ggcgagatga atcgcttgta tgttgtcgag ccgaccccct cggtaactgg ctccagcgcc
      241 gaccatcgcc tcccgcttcg ctttgtcgaa gtcgagcagt ttgcccgcgc gcttgccgcc
      301 aagctgggac tcggcggcag cggatcgctc tccgcggctg cggagaagtg gctggacata
      361 gtggtcaagg atctccagaa aaatcacggc cgctctttgg tcgtggccgg agagcagcag
      421 cccgccgaag tgcatgccct ggttcacgcc atcaacgcca cgctcggaaa catcggaaat
      481 tccgtttact acaccgagcc ggtggaagcc aatcctgtca acaatattga atccctccgt
      541 gatctttgcg ccgatatcga tgctggaagg gtctccacgc tgctcatcgt tggcggcaat
      601 cccgtctacg acgcccccca cgacttcgat ttcacttcca agctgcgcaa ggttgcccac
      661 accattcatc tcagttcgca tttcaacgaa acctccgagc actgcaagtg gcatttggcc
      721 gaatcgcatt acctggaaag ttggggtgac gcgcgctgct tcgacggcac cgtttccgtc
      781 attcagcctt tgattgctcc cctgtaccac acgcattcgg ctagcgaggt tctggcggca
      841 tttagcgaca agcctggttt gagcggctac gatgccgtac gcaatcgcct gaaggccgca
      901 aatcccaacg gcgatttcga gaagttctgg cgcaaaacgc tgaatgacgg cgtcgttgcg
      961 aattccacct atcccgcgct gagcctcacg ccgaaattca acgccgccag cctgcccgcc
     1021 attccaccgg gtaatcccgg cgaactcgag ttccttttcc gccccgatcc caccatccac
     1081 gatggccgct tcgccaacaa cggttggctg caggagctcc ccaagccggt aactaaactg
     1141 gtgtgggaca atgcggcgct ggtcggcccg aacacgtcct ggaacctcgg catcacgcac
     1201 actgttgccg cgcgtggcgg cgagcacggc cagatcgttt ccacggttgt tgacatcacg
     1261 ctgaacaaca gcaaggtgac cgcggccggc tggattttgc ccggccaggc cgaaggcgtc
     1321 gtcgttcttc ctctcggcta cggccggaag aaagccggat acaccggcac aaacaaaggt
     1381 tttaacgcct acgccgttcg cacttccaat gccctctggc gcgccacggc cggctcagag
     1441 tccgtcaaga aaaccggcga agagtattcg ctcggttgca cccaatatca tttcaatatg
     1501 gaagggcgca agattctgag caccggcact ctcgaggaat ataagaagaa tccgggcttc
     1561 gcgcacgaag gcgacgaaac tccacccaag agcctttcgc tctacgaaga gttcaagtat
     1621 cccggctacg cctggggcat ggccattgac ctgaacaact gcaatggctg caatgcatgc
     1681 gtggtggcct gcgtcgccga aaacaatatt cccgtcgtcg gcaaagacca ggttctgcgc
     1741 ggccgcgaaa tgcactggat tcgcatcgac cgctactaca acgcgaacac gcccacgggc
     1801 gatccacgta ccaacgagaa taacgccctc gccaatcccg agaccttctt tcagcccgta
     1861 ccttgcatgc agtgcgaaaa tgcgccttgc gagcaagttt gcccggtcgg cgccaccgtg
     1921 cacagcgccg agggcttgaa tgacatggtc tacaaccgtt gtatcggcac gcgctactgc
     1981 tcgaacaact gcccctacaa ggtccgccgc ttcaatttcc tccgattcca ggattgggaa
     2041 actccacagc taaagcttca gcgcaacccg gaagttaccg tgcgcagccg cggcgtcatg
     2101 gaaaagtgca cctattgcgt gcagcgcatc aacaacgctc gcatcgaagc ggaaaaacag
     2161 aatcgcccca tcgccgacgg cgaaattgtc accgcgtgcc aggcttcctg cccctccgag
     2221 gccatcgttt tcggcaacgc caacgatccc aatacccgag tcgccaagct caaggcccag
     2281 cagcgtaatt acaacgttct cggtgagcta aacagccgcc cgcgtaccac ttatttgggc
     2341 gccgtgcgca atcccaatcc tgacttggag aaggcatgag cacgcggccg ccttacaatt
     2401 ccgcggacat ccagccggt
//
LOCUS       DASTPM010000586         5137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1318212, whole genome shotgun sequence.
ACCESSION   DASTPM010000586 DASTPM010000000
VERSION     DASTPM010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318212"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFR42_10330"
     CDS             <1..296
                     /locus_tag="VFR42_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369599.1"
                     /translation="WGSPADFVKQWLAQIIFLAVAVIGIAKVVRLNLLGYFLVLALPT
                     LVMGAQELLSQPSGFYRQQGYLVMALLVMLLVWPLAQWMRSRPATSATASVPG"
     gene            549..1226
                     /locus_tag="VFR42_10335"
     CDS             549..1226
                     /locus_tag="VFR42_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0369600.1"
                     /translation="MPLPQWALALADSAGKSGEHQAVLATVTALPELDERALVVEAQA
                     GNRTAFEELVRRYDRDVLRLALNLMKRPEDARDVYQEAFLKVYRNLHRFRFECSFYTW
                     LYRIVTNVCLDHLRRRQARPEDQAPEILVNRQEEGTRDFFEVQREHRPALDPERRLIG
                     KEIRTRLAKAMERLSPRERVVFEMKHYQGLKLRAIGDALGTTEETVKNSLFRATRKLR
                     QELGELR"
     gene            1223..2311
                     /locus_tag="VFR42_10340"
     CDS             1223..2311
                     /locus_tag="VFR42_10340"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU0369601.1"
                     /translation="MSVFGSNRGRKMNCDEVTTDERSGIESHIAECAQCHAQLAEVGE
                     FQDSLSGVPQSADELDRTGTLLAQCRSELSESLDEMSAPPVEQESWRPFGFVRRWMAL
                     RPGWSAAGLLAAGAILGVQLLQWLPTNDSNWNGRAVNVSAAQKISDDQLAKMAVSGIN
                     FAPNADGVPGTLQVQLRAEQPVVLSGNLDDSDMRRVLTYVVKNSEKFDPGVRLDCLDA
                     LRAKIGDAEIRDALLDAARHDKNPAVRMKALESLREAASEEDVRDAVLESLQEDSNPG
                     VRVEAINLLVRSLEMESAMPPMPHGMAANSDEPMVEQTAPAALADSDPSVRRVLRALD
                     QLRRNDPNHYVRLRSAAALRQIGPAEVQ"
     gene            2434..4314
                     /locus_tag="VFR42_10345"
     CDS             2434..4314
                     /locus_tag="VFR42_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0369602.1"
                     /translation="MIKVSSISRIALRQLAVALFFAVIPAMAFSASEPGVHVVVAVRA
                     SEPGGRHVTRDLNGVLSTGENLTLKLNTDLGSVRIVPLQPGAMPQVRYSVHIETDAHG
                     SAAAQLLDRYALDAKNTPAGAEITGNLPQLSRGNGGAQFWVHYEISVPASYSLDITTG
                     AGDIRTFDVGGTVTLVTEGGNIVGGRIGTNFVRGQAAHARNRAARLVGKLQTQGGHIQ
                     VEDVAGDLTAFTAGGHINTGYIFGEASLQSGGGHIRAAGISGKAELSTEGGNITVGKA
                     EKLVTVKTGGGQIDFGEVRGSVRAQTGGGGIRVMYVAGPMDVESSGGSICLTRVSGSV
                     RAATDSGTITAWINPESAEKHSETVSLPGASQLTSGEGDIVVFLPKNLAANIEAIVEN
                     GGARRIEADPALSLQVAKAGQGSAAVKAWASLNGGGAVLRLKTTGGKIKLQYLDSEMA
                     LWESMMRDQSARMERELTRAALTTPARPGETASAWPTPQISMAPAAPATPVTNPRPAA
                     APEPSDVWRGSWVERMEVRLIGGVRMDADEFWTRVTRHPDPVYPEVARKGGIEGTVRL
                     QVRLTQDGKIEVQKLLEGDTVLADSAMAAVKQWRGKPMWTADGRPVDVVSTITFRFKL
                     RD"
     gene            4458..>5137
                     /locus_tag="VFR42_10350"
     CDS             4458..>5137
                     /locus_tag="VFR42_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369603.1"
                     /translation="MKLRELLVAAVVLSFALAVSAVPGFAISKEFNQSYTLQPGGTFE
                     LQNVNGTVEVQGWDKDEVEVHAVKTAKVRESDLERVAIDVHAQANRIAVLTRYPQNEG
                     VEVAVEYTIHVPHGAKVEHLGTVNGTLRVAGVSEVDELHTVNGNIEVYEADSEVRAHT
                     TNGNVHLELSHVHGVSGTVAETTNGSVILAVPANAQADLEARCLNGNFQSELPFAMEG
                     SLRPREIHG"
BASE COUNT         1084 a         1391 c         1711 g          951 t
ORIGIN      
        1 gctggggcag cccagcggat ttcgtaaaac agtggctggc tcaaatcatt ttccttgcgg
       61 tagcggtgat tggtatcgcc aaggtggtgc gtctgaacct gctgggatac ttcctggtgc
      121 tggcgcttcc gacactggtc atgggcgcgc aggaactgct ttcgcagcct agcgggtttt
      181 accgtcagca ggggtatttg gtgatggcgc tgctggtgat gctgctggtg tggccgctgg
      241 ctcagtggat gcgatcgagg ccggcgacgt cagcgacggc gtcggtgccg ggttaaagcc
      301 cggcgctaga acaccctgag cgctcggcat ccgggaaaac gacccgacga agcggtttga
      361 cttcccagaa acaaatagct gagctcattc gttgtatgag ataagaggag tggctgctta
      421 cgtgaacgat agtggtgcgt ccaaattgga tgacaccgcg gcgctgcgcg ccaggctgag
      481 ccgcgttgat tcggctaact cgccagaaat caacgttcag cgcgatcgta cgcgaataga
      541 aagcgaatat gccacttccc caatgggcgt tggctttggc cgatagcgca ggcaagtccg
      601 gcgaacacca agccgtactc gccacggtga cagctttgcc ggaattggac gaacgtgccc
      661 tcgtcgtcga ggctcaggcc ggaaaccgca cggcctttga ggaactcgtt cgccgctacg
      721 accgggacgt gctgcggctg gcgctgaacc tgatgaagcg tcccgaggat gcccgcgacg
      781 tctatcaaga ggccttcctg aaggtttacc gcaacctgca ccgcttccgc tttgaatgca
      841 gtttctacac ctggctgtac cgcatcgtca ccaacgtttg cctcgatcac ttgcgccgcc
      901 gccaggcccg ccccgaagac caagctcccg aaattcttgt aaaccggcag gaagaaggca
      961 cccgcgactt ttttgaagta cagcgcgagc accggccggc gctcgatccc gaacgcagac
     1021 tgatcggcaa ggaaattcgc acccggctgg cgaaagcaat ggaacgcctt tcgccgcgcg
     1081 agcgtgtcgt gtttgagatg aagcattacc agggattgaa gctgcgcgcg atcggcgacg
     1141 cgctgggcac gaccgaagag accgtgaaga attcgttgtt ccgtgccact cgcaagctac
     1201 gccaggaact gggagagctc cgatgagcgt cttcggttcc aatcgcggcc gcaagatgaa
     1261 ttgcgacgaa gtgactacgg acgaacgctc cgggattgag agccacatcg cggaatgcgc
     1321 gcagtgccac gcgcaactag cggaagtcgg cgaattccag gacagccttt cgggagtacc
     1381 gcagtcagcg gatgaattgg atcgcaccgg cacactgctg gcgcaatgtc gcagcgaact
     1441 ttccgaatcg ctggatgaaa tgtcagcgcc gccagtggaa caagagtcct ggcggccttt
     1501 tggttttgtg cggcggtgga tggcgcttcg tccgggctgg agcgcggcgg gattgctggc
     1561 agccggcgcg attctgggcg tgcagttgtt gcagtggctg cccaccaacg attccaactg
     1621 gaacggacgg gccgtcaatg tttcggccgc gcagaaaatt agcgacgatc agctggcgaa
     1681 gatggcagtt tccggaatca acttcgcgcc aaacgcagat ggggtgccgg gcacgctgca
     1741 ggtgcagttg cgcgcggaac agccggtggt gctctccggc aatctcgacg actccgacat
     1801 gcgccgcgtg ctgacgtatg tggtgaaaaa cagcgaaaag tttgaccccg gtgtgcggct
     1861 ggattgtctg gatgcgctgc gcgcgaaaat cggcgatgcg gaaattcgcg atgctctgct
     1921 ggacgcggcg cggcacgaca aaaatcccgc ggtgcgcatg aaggcgctgg aatcgctgcg
     1981 tgaagcggcg tcggaagaag atgtgcgcga cgcggtgctg gaatcgctgc aagaggattc
     2041 gaatcccggc gtgcgcgtgg aagccatcaa tttgctggtg cgttcgctcg aaatggaatc
     2101 ggcaatgccg cccatgccac atgggatggc ggcgaactcg gacgagccga tggtcgaaca
     2161 aacagctccc gcggcgctgg cggattcgga tccatcggtg cgccgcgtgt tgcgggcgtt
     2221 ggatcagttg cggcgcaatg acccgaatca ttacgtaaga ctgaggagcg ccgcagcgct
     2281 tcgccagatt ggcccagcgg aagttcaata acgaagttca gttgcagagc gcgtctggag
     2341 ctggttttcc gaagtcagaa agaagtgatc gaggagaacg cggagcaagt tcgcgcggaa
     2401 gttgctttgc tagagcgctt tgtcaggaaa tgaatgatta aggttagttc catttcacga
     2461 attgcccttc gccaactggc cgtcgcactt ttctttgcgg tgattcccgc gatggccttc
     2521 tccgctagcg agcctggagt gcacgttgtt gtagcagtgc gggccagcga acccggcggg
     2581 cggcatgtga cgcgcgattt gaatggagtt ttgtctaccg gtgagaatct tacgctcaag
     2641 ctgaatacgg acctgggttc tgtgcgcatt gtgccgctgc aacccggtgc aatgccgcag
     2701 gtacgctatt cggtgcacat tgaaacggat gcgcacggtt cagccgcagc gcaactgctg
     2761 gatcgttatg cgctggacgc caaaaacacg ccagctggcg cggaaatcac cggcaacctg
     2821 ccgcagctct cgcgagggaa tggtggagcg cagttctggg tgcactacga aatttccgtc
     2881 cctgcctcgt acagtctgga catcaccacc ggagcgggtg acatccgaac cttcgacgtc
     2941 ggtggaacgg tcacactggt gaccgaaggc ggcaacatcg tgggcggaag gatcggcacc
     3001 aattttgttc gcgggcaggc agcccacgct cgtaatcgcg cagcgcgcct ggtcggaaag
     3061 ctgcaaacgc agggcggcca cattcaagtg gaagacgtgg caggcgacct gacggcgttt
     3121 accgccggcg gccatatcaa caccggatac atttttggcg aagcgagtct gcagtcgggc
     3181 ggcgggcaca ttcgcgcggc cggaatcagc ggcaaagcgg aactttcgac ggaaggtggc
     3241 aacatcacgg tgggcaaggc cgagaagctg gtcaccgtaa agacgggtgg cggccagatt
     3301 gattttggtg aagtgcgcgg atcggtgcgc gcacaaaccg gcggcggtgg catccgcgtc
     3361 atgtacgtcg cgggccccat ggatgtggaa tcgagcggcg gcagcatttg cctgacgcgc
     3421 gtttccggct cggtgagggc ggctacggac tccggcacca tcacggcgtg gatcaatccg
     3481 gaaagcgctg aaaagcatag cgagacggtg agtttgccgg gcgcctcgca gctgacttcg
     3541 ggcgaaggcg acatcgtggt gtttttgccg aagaacctgg cggccaacat tgaagccatc
     3601 gtggaaaacg gcggagcgcg gcgcatcgaa gcagatcctg cgctgtcgtt gcaagtggcc
     3661 aaagctgggc aaggttccgc agcggtgaag gcgtgggctt cgctgaacgg cggcggagca
     3721 gtgctccgtt tgaaaactac cggcggcaaa atcaagctgc aatatctgga ttcggagatg
     3781 gcgttgtggg aatccatgat gcgcgaccaa tcagcacgca tggagcgcga actcacgcga
     3841 gcggcgctga cgacgcccgc gcgacccgga gagaccgctt ctgcctggcc cacgccacag
     3901 atcagtatgg ctccggctgc tccagcaact ccggttacaa atccacggcc cgcggcggcg
     3961 ccggaaccct ccgatgtgtg gcgtggcagc tgggtcgagc gcatggaagt acggttgatt
     4021 ggcggcgtgc gcatggatgc ggatgaattt tggacccgtg tgacgcggca tcccgatccc
     4081 gtttatccag aagtcgcgcg caaaggcggc atcgagggta cggtgcggct gcaagtgcgc
     4141 ttgacccagg acggaaaaat cgaggtacag aaactgctgg aaggcgatac ggtgctggca
     4201 gactccgcaa tggcagccgt gaaacagtgg cgcggtaagc cgatgtggac agcggacggc
     4261 agaccggtgg atgtggtttc aaccatcacc ttcagattca agctgcgcga ctaagcgctt
     4321 gaacgggaat ttggtcgggc acggatagca ttacccggcc aattggggct aaggtcgaaa
     4381 acgtttttcg ggagaggggg aaactccgcg acgaacggaa acgattctgg gcgcgaggtt
     4441 cctcgaaggg ggttgtgatg aagttgcgcg agctgctcgt tgcggcggtg gtgttgtcat
     4501 ttgcattggc ggtttccgca gtgccgggat tcgccatcag caaggaattc aatcaatcgt
     4561 acacgctgca acctggcggc acgtttgagt tgcagaacgt gaacggaacc gtggaagtgc
     4621 agggctggga caaggatgag gtggaagtgc acgcggtgaa aacagccaag gtgcgcgaat
     4681 ccgacctgga gcgcgtggcg attgacgtgc acgcgcaagc gaaccgcatt gcggtgttga
     4741 cgcgttatcc gcaaaacgaa ggcgtggaag tcgcggtgga gtacaccatt cacgtgccac
     4801 acggcgcgaa agtggagcac ctcggaaccg taaacggaac gttgcgcgtc gccggcgtca
     4861 gcgaagtgga cgaactgcac acggtgaacg gcaacattga agtgtacgag gcagacagcg
     4921 aagtacgcgc gcacaccacc aacggcaatg tgcatctgga gctgtcgcac gttcatggcg
     4981 tatcgggaac cgtcgcggaa acgacgaacg gctcggtgat actggctgtg ccggcgaacg
     5041 cgcaagccga tttggaagcg cgttgcttga acggaaattt ccagtccgag cttccatttg
     5101 cgatggaagg ctcgctgcgg ccgcgcgaaa tccacgg
//
LOCUS       DASTPM010000587         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_597554, whole genome shotgun sequence.
ACCESSION   DASTPM010000587 DASTPM010000000
VERSION     DASTPM010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597554"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /gene="priA"
                     /locus_tag="VFR42_10355"
     CDS             complement(<1..1929)
                     /gene="priA"
                     /locus_tag="VFR42_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU0369604.1"
                     /translation="MSDKLCNVAVAVPLRTTFTYKIPARLAAEIQVGCRVLVPFRKKS
                     TVGVVTEFIDTPPADARLREVQKSFDVVPALNPKLLELGQWIASYYVAPIGEVYRTML
                     PPLTELRAQQRVVLTEAGRAALEPAPSANSASKSDFFLQSNSPTAVSLPTEALPFLQK
                     LKSAKSALLLPAVVRSGVPLSDLLKLQRRGLLEIRQDMQDRKRRMQRIFAWKETPASE
                     TCKPLSEKEQRLKRLLSVERGPLPLAQLLKLSGLSRTVMERLFRQGRLESWEEPLDPA
                     EDPFDIGYTPPAHALNAEQDRAFAAIRARFELGEFGVQLLHGVTGSGKTEVYLRAVQD
                     TLARGKTAVVLVPEIALTLWIGRQCRAWFGAHFEGVAVLHSALSDVERAREWWRVRNG
                     EARVVVGTRSAIFAPVENLGLIIVDEEQESSYKQEESPRYHGRDVAIVRAKLENALAL
                     LGSATPSLETYHHARGGKYELLSLTQRVADRALPSVEIVDLREEFKQTHLTSPISSAL
                     RKGIEECLACNTQALVLINRRGYSWSVICRSCGASVQCANCSISMIHHKYRNRLECHY
                     CGYTQGIPKKCPKCDSQYVYFFGEGSEQLEERLRKEFPRARLARLDRDTARTKRQYQE
                     TLGAFAEGALDILVGTQML"
     gene            complement(2026..>2509)
                     /locus_tag="VFR42_10360"
     CDS             complement(2026..>2509)
                     /locus_tag="VFR42_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0369605.1"
                     /translation="AGEKSPQSTPTSLATGPQNPVRNSTGILWALAAALGFGILFWLL
                     GTRIIPRTGALATIWMIRVTGSFITLAILLAQRTSLRVKNKRASAQLYSMGFFDTAAF
                     TLSNLGMRIEQVAIVSVLGSLYGAVTVVLSALFLRDRPAALQWLGIVSIFSGIILMNR
                     "
BASE COUNT          514 a          600 c          911 g          484 t
ORIGIN      
        1 gagcatttgg gtgccgacga ggatatcgag cgctccttcg gcgaatgcgc ctaacgtttc
       61 ctgatactgg cgcttggtgc gggcggtgtc gcgatcgagt cgagcgaggc gagctctggg
      121 aaattccttg cgcaggcgtt cctcgagctg ttcggaaccc tcaccgaaaa aataaacata
      181 ttgcgaatcg cacttgggac attttttcgg gatgccctgg gtgtagccgc aatagtggca
      241 ttcaaggcgg ttgcgatatt tgtggtggat catgctgata ctgcagttag cgcactgcac
      301 ggaggctccg caactgcggc agataacgga ccaggaatag ccgcgccggt tgatgaggac
      361 gagggcctgg gtgttgcagg cgaggcattc ttcgatgcct ttgcgcaacg cggaagagat
      421 ggggctggtc agatgggttt gcttgaactc ttcgcggaga tcgacgatct cgacggacgg
      481 aagagcgcga tcggcgacac gctgggtgag cgaaaggagt tcatatttgc cgccgcgggc
      541 gtggtgatag gtctcaagtg aaggcgtggc ggaaccgaga agcgccagcg cgttttcgag
      601 tttggcgcgg acgatggcga cgtcgcgtcc gtggtagcgc ggactttctt cctgcttgta
      661 gctggattcc tgctcttcgt cgacgatgat gaggccgaga ttttcgacgg gcgcgaaaat
      721 cgcggagcgt gtgccaacga ccacgcgggc ttcgccattt cgaacgcgcc accattcgcg
      781 agcgcgctca acatcgctga gcgcggagtg aagaacagcg acgccttcga aatgagcgcc
      841 gaaccaggcg cggcactggc ggccaatcca aagagtgagc gcgatttccg ggaccaggac
      901 gacggcggtt ttgccgcgcg cgagcgtgtc ctgcacggcg cgaaggtaga cttcggtttt
      961 gccgctgcca gtgaccccgt ggaggagctg gacgccgaat tcgccgagtt cgaagcgggc
     1021 gcggatggcg gcgaaggcgc ggtcctgttc ggcgtttagt gcatgggcgg gaggagtgta
     1081 gccgatgtcg aagggatctt cggcgggatc gagcggctct tcccaggatt cgagtctacc
     1141 ttgacgaaag aggcgctcca tgacggtgcg ggaaaggccg gaaagtttca gaagctgtgc
     1201 cagggggagc gggccacgtt cgaccgaaag aagcctcttg aggcgctgtt ctttttcgga
     1261 caggggcttg caggtttcgc tcgctggcgt ttctttccag gcgaagatgc gctgcatgcg
     1321 gcgcttgcga tcctgcatgt cctggcggat ttcgagcaaa ccacgacgct ggagtttgag
     1381 aaggtcggac agcggaacgc ctgagcggac gactgcgggg agaagaagag cggactttgc
     1441 ggatttgagt ttttgcagga acggcagcgc ttcggttggc agagatacgg cagttggtga
     1501 atttgattgc aaaaagaagt cggattttga cgcagagttc gcagagggcg cgggctcgag
     1561 agcggcgcga ccggcctcgg tgaggacgac gcgctgctga gcgcggagtt cggtgagtgg
     1621 cggcagcatg gtgcgataga cttcgccgat gggcgcgacg taatacgagg cgatccattg
     1681 gccgagctcg agaagtttgg gattgagagc aggaacgacg tcgaacgatt tctgaacttc
     1741 acgaagcctg gcgtcggcag gcggtgtgtc gatgaattca gtgacgacac cgacggtgga
     1801 ttttttgcgg aacggaacga gcacgcggca gccgacctgg atttcggcgg caagccgggc
     1861 gggaattttg taggtgaaag tagtacgcaa gggaacggcg acggccacgt tgcagagttt
     1921 gtcggacatg cgagggagta gtttagcagg ggaagcgtgc ggggctgatt tcgcgttgcg
     1981 gatcagatgg attcaaacgc agaggtcaca gagtaaaacc aggccctagc ggttcatcag
     2041 gatgattccg gagaagatcg aaacgatgcc gagccattgg agggcggcgg gccggtcgcg
     2101 caggaaaagg gcggaaagca cgacggtgac ggcgccgtag agcgagccga ggacgctgac
     2161 gatggcgact tgctcgatgc gcatgccgag gttgctgagc gtgaaagcgg cggtgtcaaa
     2221 gaaacccatg gagtagagct gggcactggc gcgtttgttt ttgacgcgga gagaagttct
     2281 ttgcgcgagc aagatggcga gcgttatgaa actgccggtc acgcggatca tccagatcgt
     2341 ggcgagagcg ccggtgcggg gaatgatgcg ggtgcccagc agccagaaaa gaatgccgaa
     2401 gccgagcgcg gcagccaggg cccacaggat accggttgag tttcgcacgg gattttgtgg
     2461 tccagtggca agcgaggttg gcgttgattg tggagacttt tcgcccgcg
//
LOCUS       DASTPM010000588         4873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_769614, whole genome shotgun sequence.
ACCESSION   DASTPM010000588 DASTPM010000000
VERSION     DASTPM010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769614"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..3068)
                     /locus_tag="VFR42_10365"
     CDS             complement(336..3068)
                     /locus_tag="VFR42_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0369606.1"
                     /translation="MRPLRATWIRFRNSFRSRKLNHDLDDELTAHLDLHISDNLRAGM
                     TPAEARRKALIKLGGLDQTRDDYRGAAGFPFLESLLQDARFAIRTLRKSPGFTAVAVL
                     TLGLGIGANTGIFTILHQVLLQRLPVPHPEQLVLLYGPGPRQGHVSSDESHTDGAESF
                     SYPMYLNLRDRNEVFLGLAAKDSATVNLSFHGSTEQADADVVTGNYFDTLGVRAAFGR
                     TLEPADSATVGGNPVVVLSYAYWKNHFAGDLAILNQTILVNNRPMTIVGVVQPGFQGI
                     QRGFVPQVYIPITQPLSTEPAGFENHKDYWIKLIGRLKPGVTLQRAQVGLAPLYGALL
                     RDELPLQTGWNDTEKAQFLDKKIVFRPGARGRPILEASAGPQLVALMCLVAAVLFIAC
                     ANVAGLLTARGAVRQREIGARLSLGASRARLIRQLIVESLLLSTAGTLLGLLIANFTS
                     SALARFADENGIATGLSGALNTPVLLFACALMFLCGILFGVAPALRATRVELVSTLKD
                     QTGTLASGSSNTRLRKALVVCQVAMALLLVTGAGAFARSLYNVKHIDLGLHPSHVLQF
                     TVAPLLNGYDQTRTFTFFRALEDRISALPSILSISAAQEPLIADSDRGSNVTVEGESP
                     AEAGSRHALRNAIAPGHFSNLGIPLLKGREFSRADGSGSPKVAIINESFAKTFFPATD
                     PVGRHMKFGGGPGTLNMEIVGVVKDSHHSGVKEQIQPFVYIPYSQEKNVGALTFYVRT
                     AQDPLTVAAAVRHAVSELDSSLPVADLRTFERQIDRQFATDRLIALLAGIFGALAAVL
                     AAIGIYGLLAYTVAQRTREIGIRMALGADARLIVALLLKDVAVLIVVGVILGLPVSYA
                     LGRLIDSLLYSAKAFEFLGIVGALALLLLVTLLASYLPARRATRVDPLVALRYE"
     gene            complement(3093..3308)
                     /locus_tag="VFR42_10370"
     CDS             complement(3093..3308)
                     /locus_tag="VFR42_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369607.1"
                     /translation="MRPLRATWTRFRNLFGTRKLDRALHAELAAAFDPATFVGAPLAL
                     LAAGVLACYIPARRATRVAPLAALRYE"
     gene            complement(3305..3649)
                     /locus_tag="VFR42_10375"
     CDS             complement(3305..3649)
                     /locus_tag="VFR42_10375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0369608.1"
                     /translation="MGDKQDVWQGTLALMILKTLQSMGALHGYGIARRIEQTSGDLLS
                     VNYGTLYPALLKLEQDGYISSEWGVSENNRKAKFYKLTRAGRKQLEKEAREWYQATAI
                     LARFLNAEEPNS"
     gene            complement(3773..4741)
                     /locus_tag="VFR42_10380"
     CDS             complement(3773..4741)
                     /locus_tag="VFR42_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0369609.1"
                     /translation="MRTVLSLLLFALFSSYAPAQAPPAAGGSLPFTLKPLGHGVYAAI
                     DNSKGEAGANAGFVIGDEGVVVIDTFENAPAAQALLGEIRMLTKLPIKFVVNTHYHLD
                     HVAGNAVFAKEGAAIVAHRNVPSWIHTENLKFFGKDIKPEQKSEVENLAAPDILYDSS
                     LELRVGAPPIRVEFYPGHTGGDSVVNVQQEKTAVIFCGDLFWRQTLPNLIDASTLPWL
                     ETLATLSKFSAESPITFVPGHGDPGTAADVTAFHSYLSDLRDWVAAAKKDGKAGASLM
                     DTVLPEVKQKYGSWQFFDYFAKRNIADVEAELNGSKRIPIPIAKTH"
BASE COUNT         1058 a         1195 c         1688 g          932 t
ORIGIN      
        1 tccgtgaaga gaaccgatgg tggccagggt ctgcaggacc atgagctcga gcgtcccctg
       61 gaggaggtcg agtttggaac cgggcatacg agtgcgctcc tgtgcgatgg ccacagaaca
      121 gtggtgtgtt cctgtgcgta tgccacaggg ggtcctgttg ggagcccgca ggtacagacg
      181 aaggttagcg gcaaaaagtt tggcgacccg gacaaatttt aagggccgga gtcaatcgcg
      241 ggattattcc ttagaaggta gccataaccc ctttattttc agagggcgca ttgcggctat
      301 tttgatgatt cgcttgtggt tcgcagaacc atgatttatt cgtaacggag agcgaccaac
      361 gggtctactc gagttgcgcg gcgcgctgga agatatgagg cgaggagagt tacgagcagg
      421 agaagggcaa gagcgccgac gatgccgaga aattcaaatg ctttggcgct gtagagcaag
      481 gaatcgatca gacggcccaa cgcataagag accggcaagc cgaggatgac gccgacgacg
      541 atcaggaccg cgacgtcttt cagcaatagc gcaacgatga gtctggcgtc ggcgcccagg
      601 gccatgcgaa tgccaatttc gcgggtgcgt tgcgcaaccg tgtaggcgag caggccgtag
      661 atgccgatgg cggccaggac tgcggcgaga gctccgaaga ttccggcgag caaggcgatg
      721 aggcgatcgg tggcaaactg ccgatcgatt tgccgctcaa acgtgcgcag atcggcgacg
      781 gggaggctgg agtccagttc ggaaacggcg tgacgcacgg cggctgcgac cgttagggga
      841 tcttgcgcgg tacgcacata aaaagtgaga gcaccgacgt ttttttcctg cgaataggga
      901 atgtaaacga aaggttgaat ctgttccttg acgccggaat gatggctgtc tttgacgact
      961 ccgacaatct ccatgttcag cgtgccgggg ccgccgccaa atttcatgtg gcggccgacg
     1021 ggatcggtgg cagggaagaa agttttggcg aatgattcgt tgatgatggc gactttaggg
     1081 cttccgcttc catcggcgcg agagaattcg cggcctttca gaagaggaat gccgagattc
     1141 gaaaaatggc ccggagcgat ggcattgcgc aaagcgtgac gcgagccggc ttcagcgggc
     1201 gattcgcctt cgacggtgac attggagccg cggtcgctgt cggcaatcaa aggttcctgc
     1261 gccgcactga tggaaagaat gctgggcaga gcggaaatgc gatcttccag agcacgaaaa
     1321 aaagtgaagg tgcgagtttg gtcgtagcca ttgaggagcg gggcgacggt gaactgtaga
     1381 acgtgagacg gatgcaggcc gaggtcaatg tgcttaacgt tgtataggct gcgagcaaac
     1441 gcgcccgcgc cggtaacgag caagagagcc atcgcgacct ggcaaaccac cagcgctttg
     1501 cggaggcgag tgttactgga accggaagcc agcgtgccgg tctgatcctt cagagtggag
     1561 acgagttcga ctcgggtagc gcgaagagca ggtgcgacgc caaaaagaat gccgcagaga
     1621 aacatgagag cacaggcgaa aaggagaacg ggcgtgttga gtgcgccgga aagcccggtg
     1681 gcgatgccat tttcatccgc aaaacgcgcg agagcggagc tggtgaagtt tgcgataagc
     1741 aggcccagca aagtgcctgc tgtggatagc aagagcgatt cgacgatcag ttgacggatc
     1801 aggcgggcgc ggctggctcc caaggataac cgcgcaccga tttcccgctg acgtactgcg
     1861 ccgcgcgctg tcaggagtcc ggcgacgttg gcgcaggcga tgaacaggac ggcggcgacc
     1921 aggcacatca gagcgacgag ttgcgggccg gcggatgctt cgagaatggg gcggccgcgc
     1981 gcgccggggc ggaaaacgat ttttttgtcg aggaactgag ctttttcggt gtcgttccag
     2041 ccggtttgca gcggcaattc gtcacgaagc agcgcgccgt atagtggggc caggccgact
     2101 tgcgcgcgtt gcagcgtcac gccgggcttg agcctgccaa tgagcttgat ccagtagtct
     2161 ttgtgattct cgaagccagc gggctcggtg ctcaagggct gcgtgatggg aatgtaaacc
     2221 tgcggcacaa agccgcgttg gatgccttgg aagcccggct gaacgacgcc tacgatggtc
     2281 atcggccgat tgttgacgag gatagtttgg ttcaggatgg cgaggtcgcc cgcgaaatga
     2341 tttttccagt atgcgtagga caacacgacg accggattgc cgccgacggt tgcggagtcg
     2401 gcaggctcca gagttcggcc gaacgcggcg cgaacgccca gggtgtcgaa ataattgccg
     2461 gtaaccacgt cggcgtccgc ttgctccgtg gaaccgtgga aggagagatt gacggttgcg
     2521 ctgtcttttg cggcgagccc caggaagact tcgttgcggt cgcgaagatt cagatacatc
     2581 ggataagaga aagactcggc gccgtcggtg tgactttcgt cagagctgac gtgtccttgc
     2641 cggggaccag ggccataaag aaggacgagt tgttcggggt gaggcacggg aaggcgttgc
     2701 aacaggactt gatgaaggat ggtgaaaatg ccagtgttgg cgccgatgcc gaggccgaga
     2761 gtcaagacag cgactgccgt aaagccggga cttttgcgga gcgtgcggat ggcgaagcgc
     2821 gcgtcttgga gaagggattc gagaaatggg aagccggcgg cgccgcgata gtcgtctctg
     2881 gtttgatcga ggccgccaag tttgataagt gccttgcggc gggcttccgc gggagtcatg
     2941 cccgcgcgca ggttgtcgga gatgtgcagg tcgagatgag cggtcagttc atcgtcgagg
     3001 tcgtgattca gcttgcgcga gcggaacgag ttgcgaaaac gaatccaagt cgcgcgcagc
     3061 ggtctcatac gtttggctcc gataaagaag tcctattcat agcgcagtgc ggccagaggg
     3121 gcgacgcgcg ttgcgcggcg ggcggggatg taacaagcga gcactccggc ggccagcagc
     3181 gcaagcggcg cgccgacaaa tgtggccggg tcgaacgctg cagcgagttc cgcatggagg
     3241 gcacgatcga gcttgcgtgt gccgaacagg ttgcggaagc gagtccaggt agcgcggagt
     3301 ggcctcatga gttgggctcc tcggcgttaa ggaagcgggc gaggatggcg gtagcctgat
     3361 accattcgcg ggcttctttt tcgagttgct tgcggccggc gcgggttagc ttgtaaaact
     3421 tggccttgcg gttgttttca gacacgcccc attcagagga gatgtagccg tcctgctcga
     3481 gtttaaggag ggcagggtag agcgtgccgt agttgacgga gagcaaatct ccgctggttt
     3541 gttcaatgcg gcgggcgatg ccgtagccgt ggagcgcgcc catggactga agggttttga
     3601 ggatcatcag cgcgagagtg ccttgccaga cgtcctgctt gtcgcccatg gcggcttctc
     3661 cggactgggg gagcggggtt ttgcgcgctc ctatggctta tacataggaa gataggcgcg
     3721 ttcctatggg aaagcaatag aaaagtagag cgccggcacg ttgagcggcg cgctagtgcg
     3781 ttttggcaat gggaatcggg atgcgcttgg agccgttgag ttcagcttcg acgtcggcga
     3841 tattgcgttt ggcaaagtag tcgaagaatt gccaggagcc gtatttttgt ttcacctcgg
     3901 gcagcacggt gtccatgagc gaagcgccgg ccttgccgtc cttcttcgca gcggccaccc
     3961 aatcgcgcaa atcggagaga tagctgtgga aggcggtaac atccgccgcc gtgccggggt
     4021 cgccatggcc gggaacgaag gtgatgggcg actcggcgga aaacttggag agcgtggcca
     4081 gcgtttcgag ccagggaagc gtggaggcat caatcaaatt aggaagagtc tgccgccaga
     4141 agaggtcacc gcagaaaata accgcggttt tctcctgttg cacattgacg acagagtcgc
     4201 cgccggtgtg gcccggataa aactccacgc ggataggtgg cgcgccgacg cgaagctcca
     4261 gggacgaatc gtacaagatg tcgggtgcgg cgagattttc gacctcggat ttctgttcgg
     4321 gcttaatgtc tttgccaaag aacttcagat tctccgtgtg aatccacgag ggcacgttgc
     4381 ggtgagcgac aatcgcggcg ccctctttag cgaaaacagc gttgcccgca acgtgatcga
     4441 gatggtagtg ggtgttgacg acgaatttga tcggcagctt ggtgagcatg cgaatttcgc
     4501 ccagcagcgc ctgcgctgca ggagcattct cgaaagtgtc gatgaccacg acgccttcat
     4561 cgccaatcac gaagccggca ttggctccag cttccccttt ggagttgtca atcgcggcgt
     4621 aaacgccatg gccgagaggc ttgagggtga atgggagcga tccaccggcg gcgggaggag
     4681 cttgcgccgg agcgtatgag gagaaaagcg caaagagaag gagactcagg acagttcgca
     4741 tggagaacat cgtaagtcgg aagtgaaaaa aggaaaacgg aaaatcgcag cggcttacga
     4801 ttataggcgt tcggcgattt cgaggatctt ggtgacggag ttccagttgc ggcccgtgcc
     4861 ggccgtttgc agg
//
LOCUS       DASTPM010000589         5266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1261048, whole genome shotgun sequence.
ACCESSION   DASTPM010000589 DASTPM010000000
VERSION     DASTPM010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261048"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFR42_10385"
     CDS             <1..889
                     /locus_tag="VFR42_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369610.1"
                     /translation="KLKDGKKLYGVIVGYEDNMFRVKTDFGFVLVEKDKIASIVPAAG
                     AGNEPAAKSAGKAPETKEKATVDAESTNKAAADVAGKAKISDGPVRPSLPASGNVEKL
                     TPPQIKANPAPGTSVVATAQPPAPPKEEELPANHEEVQGTQYINFSHGFRMYKAPSWS
                     LIEDARQALPNAIVAMGTSNESTLMVVGQEKLKDSLDATAFSVEKRLKEIYTNYRLIS
                     QRKMAVGGAPATEYKYRGTADEHDWSGTLVVVGRGKDVLTILGMTYADSDLIQIQENV
                     IARAIASTSFDVPKYAAEK"
     gene            complement(1097..2002)
                     /locus_tag="VFR42_10390"
     CDS             complement(1097..2002)
                     /locus_tag="VFR42_10390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0369611.1"
                     /translation="MDFDQLTTFVYVAKLKSFSRAGQKVFRSQSAVSAQIRQLEQAYR
                     AKLLDRSAKSVELTPAGEVLFDYAERLLRLRDESVQLVADRGNVVQGPVVFGANEATC
                     LYLLPDIFAEFQRKYPQVHISIYRNFSHKILQRVEDGSVDVGIVTLPLKSPNLKVHHI
                     YKDRMRFMVSSKNPLASRNEVTLEEVAGQPLIFPRTGFTRQVLDKLFRPYRSRVHVAM
                     ELPSIGMIKRFVGADAGVSFISESFAKDQVKAGEVKLLSVDGVDLFRELGLVYRRDRS
                     LPRAAQALIGLIREHKNLSGSPDPA"
     gene            complement(2076..>5266)
                     /locus_tag="VFR42_10395"
     CDS             complement(2076..>5266)
                     /locus_tag="VFR42_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEU0369612.1"
                     /translation="GPGEMIIAKPESGLLLRWREILKRLGGHQSKLTPPTVRRLDASV
                     TPQPSAQVEAPRRTAAACGWTEDQFRILFSALVHGKEADWSMGDDAPPAFLSSLPRTL
                     WDYCKQRFAQVTNPPIDPLRETHVMSLEVRLPGNAAAPGGVVLPGPILTQGELADLAA
                     QFGPVHSINFTFPPNTGVPGARGAIAQCLSTPLGGHARPGLFLLSDRGIAQDRAALPA
                     LLATAALWKSMVREDLWDVPLVVETAQAFDTHHVALLVAAGASAVVPYLTEQFAEAEE
                     PAGFNKVRNATYAGLRKVLARMGVSTLASYRNSYLFEIVGLSEDLCAEFFEDAADFTG
                     QKTLDDLLADYLRMHSESFAKTGEDMPDAGLYRFRKGAELHANSPEVVRRIHAHVKAP
                     QAGKYPAFEELSDANGVVFLRDLLDTVASTPISMDDVEPAESILKKFSTQAMSLGSLS
                     PEAHRTLAVAMNEIGGRSNTGEGGEDPDLWTNEPHAANKIKQVASGRFGVTSDYLVHA
                     QELEIKMAQGSKPGEGGQLPAKKVTPYIARIRHATPGTPLISPPPHHDIYSIEDLAQL
                     IHDLRAVNPRARIGVKLVSGAGVGIIAAGVAKAGADVITISGHNGGTGSSPLTSIKNT
                     ALPWEIGLRETHETLVRAGLRSRLSLRVDGGLKFSRDIIIGAILGADEFGFGTASLLA
                     IGCVMARQCHLNTCPVGIATQDETLRARFTGKPEMVISYFRALAEEVRKKLADLGANS
                     LSELKGAYETLRPRSPNYAETPALALESNMSFRVAPQQTPGLHASLIEAPIVQMENSD
                     TESHITPIRNSDRSIGANLSGELARIRAAGDNSVHAVFQAFHGSAGQSFGAFLGVGID
                     FKLHGEANDYVGKGLSGGIISITAGSNASRRGDVLAGNTVLYGATSGQLFVAGRAGER
                     FAIRNSGALTVVEGVGQHGCEYMTGGVVLVLGPLGLNFGSGMTGGLAYVLRAEAEDVL
                     HRDFVSIEEISVQEDVWLRQVLQEHLDRTGSPRAARLLSRRGALPMLRVQPIHLQGTV
                     EDTWQPVLAKLGANLAIPLESHHIATSHAVSV"
BASE COUNT         1155 a         1365 c         1770 g          976 t
ORIGIN      
        1 aaaactgaag gacggcaaga agctttacgg cgtgattgtc gggtacgaag acaacatgtt
       61 tcgggtgaag acggacttcg gttttgtgct ggtggagaag gacaagattg cgtcgattgt
      121 tccggcggcg ggcgcgggca acgagccagc ggccaagtct gcgggcaagg cgccggagac
      181 caaggaaaaa gccaccgtag acgctgaaag cacgaacaag gccgccgcgg acgttgccgg
      241 caaggcaaag atatccgatg ggccggtacg gccttccctt cccgcatccg ggaatgtgga
      301 gaagttaacg cctccgcaga ttaaagcaaa ccccgcgccg ggaacttcag tagtggcgac
      361 agcgcaaccg cctgcgcccc cgaaggaaga ggaactgccg gcaaatcacg aggaagtgca
      421 aggcacgcag tacatcaact tttcacatgg ttttcggatg tacaaagcgc caagctggtc
      481 gctgatcgag gatgcgcggc aggcgttgcc gaatgcgatt gtggcaatgg gtacttcgaa
      541 tgagtccacg ttgatggtag tgggccagga gaagctcaag gactcgctgg atgcgacggc
      601 gttctccgtg gagaagaggc tgaaggagat ttacaccaat taccggctga tttcccagcg
      661 gaaaatggcg gtaggcgggg cgccggcgac ggagtacaaa tatcgcggga cggccgacga
      721 acatgattgg tcgggaacgc tggtggtggt tgggcgcggg aaagacgtac tgacgatatt
      781 agggatgacg tacgcggaca gcgatctgat tcagatccag gaaaacgtga ttgcgcgggc
      841 gattgcgagc acgagctttg atgtgccgaa gtatgcggcg gagaagtaga gacgcgaagc
      901 ccgcttccga aacacacttg aggcgctatg acggagagat ccttcggcga tcttcgttcg
      961 cccctggatg acgattcgcc gattcgcccg tgtatccgca gggtcctcgg atgaccgcga
     1021 cgcgtcgaga tctacgagaa atgttcggcc gagaaagcgg gagcttggct cccatactcc
     1081 aaataagacg caggaatcag gcggggtcag gggagccgga aaggttcttg tgttcgcgga
     1141 tgaggccgat gagggcttgc gccgcacgag gcaggctgcg atcgcgacgg tagacgaggc
     1201 cgagttcgcg gaaaagatcc accccgtcga cgctgagcag cttcacttcc ccagctttca
     1261 cctgatcttt ggcaaagctt tcgctgatga aggaaacccc ggcgtcggcg ccgacgaagc
     1321 gcttgatcat accgatgctg ggcagctcca tggcgacatg gacacgcgag cgataaggcc
     1381 ggaacagttt gtcgagaacc tggcgagtga agccggtacg cggaaaaatc agcggctggc
     1441 cagcaacttc ttcgagggta acctcattgc ggctggccaa gggattcttg gagctgacca
     1501 taaaacgcat gcggtcttta taaatgtgat ggaccttgag gttgggagat ttgagcggca
     1561 gagtgacgat accgacatcc acagaaccgt cttcgacgcg ctgcagaatc ttgtgactga
     1621 aattgcgata gatgctgatg tggacctgcg ggtatttgcg ctgaaattcc gcaaaaatgt
     1681 cggggaggag atagaggcag gtagcctcat tggcgccaaa gacgaccgga ccctggacga
     1741 cgttgccgcg atcggcgacg agctgaacgg attcgtcgcg caggcgaagc aggcgttcgg
     1801 cgtaatcgaa gaggacttca ccggcgggag tgagctcgac ggacttggcg gaacgatcca
     1861 gaagtttggc tcgataagct tgctccagct gacgaatctg cgcactgacg gccgactgtg
     1921 agcgaaaaac cttttgcccc gcgcgggaaa aacttttcag cttggcgaca taaacaaaag
     1981 ttgtgagttg atcgaagtcc atagtcggcg gttgtgcccg gattatagcg gaaacacgga
     2041 cagaaaaaga agcagtcctc cctgtgtgag agaaatcaaa cggaaaccgc gtgcgaagtg
     2101 gcgatgtggt gcgattccaa gggaatagca agattggcgc cgagcttcgc cagcacgggt
     2161 tgccaagtgt cttccacggt gccctgcaag tggatgggct gaacgcggag catgggcagc
     2221 gcgccacggc gagaaagcag tcgtgccgcg cgaggacttc cggtgcgatc gagatgttcc
     2281 tgcagaacct ggcgcagcca gacatcttcc tggacggaaa tttcttcgat agagacgaag
     2341 tcgcgatgga gcacgtcttc cgcttcggcg cgaagaacgt aggccaggcc accggtcatg
     2401 ccggaaccga aattgaggcc cagggggccg agaacgagca ccaccccccc agtcatatat
     2461 tcgcaaccat gctggccgac gccttcgaca acggtgagag cgccactgtt gcggatggcg
     2521 aagcgttcac cggcgcggcc agcgacaaaa agttggccgg aggtagcgcc gtaaagaacg
     2581 gtgttgcccg cgaggacatc gccacggcgc gaagcgtttg agcccgcagt gatggagatg
     2641 attccgccgg agagaccctt gcccacgtag tcgttggctt cgccgtggag cttgaagtcg
     2701 atgccgacgc cgaggaaagc gccgaaactt tgtccagcgc tgccgtggaa tgcctgaaag
     2761 acagcgtgca ctgagttgtc accggcggcg cgaatgcggg caagttcgcc gctgagattt
     2821 gcgccgatgc tgcgatcgga gttgcggatg ggagtgatgt gcgattcggt gtctgaattt
     2881 tccatctgga cgatcggcgc ttcaatgagc gaagcgtgca ggcccggagt ctgctgagga
     2941 gcgacgcgga aagacatgtt ggactcgagc gcgagcgccg gagtttccgc ataattcggc
     3001 gaacgggggc gcagggtttc gtaagcgccc ttaagttcgc tgagcgaatt ggcgccaagg
     3061 tcggcgagtt ttttgcgaac ttcttcagcc agggcgcgga aataggagat gaccatttcg
     3121 ggtttgccgg tgaagcgggc gcgcaacgtt tcgtcctggg tggcgatgcc gaccgggcaa
     3181 gtattgagat ggcattgtcg ggccatgacg caaccgatgg cgagaagcga agccgtgcca
     3241 aaaccgaatt cgtcggcacc aagaatcgcg ccaatgatga tgtcgcgcga aaatttcagg
     3301 cctccgtcca cgcggagaga gagtcgtgaa cggaggcccg cgcgaaccag agtctcatgg
     3361 gtttcgcgca aaccgatttc ccacggcaga gcagtatttt tgatggaagt aagcggcgaa
     3421 gaaccagtcc cgccgttgtg cccgctgatg gtgatgacgt cggcgccagc cttggctacg
     3481 ccggcggcaa taatgccgac gcccgcgcca gagacgagtt tgacacctat acgagcgcgc
     3541 ggatttacgg cgcgcagatc gtggatgagc tgcgccaggt cttcaatgct gtaaatgtcg
     3601 tggtgcggag gcggagaaat aagcggcgtg ccgggagtag catgccggat gcgcgcaatg
     3661 tacggcgtga cttttttggc gggaagctgg ccgccttcgc cgggcttgga gccctgcgcc
     3721 attttgattt cgagctcctg cgcgtggacc agatagtcgg aggtgacacc gaagcggccg
     3781 gaagcgactt gcttgatttt gtttgcggcg tgcggttcgt tggtccagag gtcgggatct
     3841 tcgccgcctt cgccggtgtt gctgcggccg ccgatttcgt tcatagcgac cgccagcgtg
     3901 cggtgtgctt cggggctgag cgagccaagg gacatggctt gcgtactgaa ttttttgagg
     3961 atggattcgg ccggctcgac gtcatccatg gaaatcggcg ttgacgcaac tgtgtccagc
     4021 aaatcgcgca ggaaaacgac accgttggcg tcggaaagct cctcaaaggc agggtacttc
     4081 cctgcttgcg gcgctttgac gtgagcgtga atgcggcgaa cgacttcggg tgaattggcg
     4141 tgaagctccg cacctttgcg gaagcgataa aggccagcgt cgggcatgtc ttcgcccgtt
     4201 ttggcaaagg attcactgtg catgcggaga tagtcggcga gaagatcgtc gagagttttt
     4261 tggccggtaa agtcagcggc atcttcgaag aactcggcac agagatcttc ggagagaccg
     4321 acgatctcga agagatagct gttgcgatag ctggcgagcg tggagacgcc catgcgggcg
     4381 agcactttgc gaaggccggc gtaagttgca ttgcgaactt tgttgaaacc ggcgggttct
     4441 tcggcttcgg caaactgttc cgtaagatac ggaacgacag cgctggcgcc tgcggcgacc
     4501 agcagcgcaa cgtgatgagt gtcgaaagct tgcgccgttt ctacgacgag cgggacgtcc
     4561 cacagatctt cgcgaaccat ggatttccag agcgcagcag tagccaacag agccggaagc
     4621 gccgcgcgat cttgcgcgat gccgcgatcg gacagcagga agagccccgg gcgcgcgtgg
     4681 ccacccagag gggtagataa gcactgggcg attgcgcctc gtgcacccgg gactcccgtg
     4741 ttgggaggaa acgtaaaatt gatcgaatgc actgggccga attgcgcggc cagatcagcg
     4801 agttcgcctt gcgtgagaat cgggccgggg agtaccactc cgcccggagc agcggcattg
     4861 ccgggcaggc gaacttcgag cgacataaca tgcgtttcgc gcagcggatc gatcggcgga
     4921 ttggtaactt gcgcaaaacg ctgcttgcag taatcccaaa gcgtgcgcgg cagcgacgac
     4981 aggaacgcgg gtggtgcgtc atcgcccatg ctccagtcgg cttctttgcc gtggacgagc
     5041 gcggagaaaa gaatcctgaa ttgatcttcg gtccagccgc aggcggccgc cgtgcgacgc
     5101 ggcgcttcga cttgtgcgga gggctgcggc gtgacggacg cgtcgaggcg gcggaccgtt
     5161 ggaggcgtga gcttggactg atgtccgccg agccgcttga ggatttcgcg ccagcgaagc
     5221 agcaagccgg attcgggctt agcgataatc atttcgcctg ggccga
//
LOCUS       DASTPM010000590         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1203419, whole genome shotgun sequence.
ACCESSION   DASTPM010000590 DASTPM010000000
VERSION     DASTPM010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203419"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2274)
                     /locus_tag="VFR42_10400"
     CDS             complement(<1..2274)
                     /locus_tag="VFR42_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU0369613.1"
                     /translation="MRFLTPTGNKRLNELTGFLCLTFALLVAAALFTYWPGDASFNVS
                     SSRDGQVHNLIGPFGAYTADAMFQLLGYSAFLIPMAALWLGIKWFRSRPVESQKATLF
                     GYALLLLFLPALLTLIHFPDVRGAIPSGGMIGALLSGALTAGFNKAGALIVTLALLVT
                     ATFLTTTFSFGRAHAWASGPRGPIGAMDKLGVLQRAQARWHDWRERREQDRMRRRVEA
                     NRLEGRKPIKSQAGAAVLDEEPQTIRLQDQSDIFKGKGKSATEEEDEEDEKNHKAPIL
                     FLNQDKPEKAKKSGDPKIAKGNTNYKLPSVSLLREGERSQKLDEDELKERARAIEEKC
                     LEFDVQGRITQINPGPVVTTFEFKPEAGIKYSRIIGLTEDLCLALQAESILIERIPGK
                     STIGIEVPNLNRQTIALREIIEAQEFVGSPSKLTLAMGRDLHGRIRVTDLASMPHLLI
                     AGSTGTGKSVFINSLMMSILYKATPDDVKMVLVDPKRLELNLYEGIPHLIAPVVTDPK
                     VASNVLRNATREMESRLKLLAQRGVRNIDQYNRTFQKQQSLSLFENVEDSEHKPLPYL
                     VIIIDELADLMMVDTNNVEESITRLAQMARAVGIHLILATQRPSVDVITGLIKANFPA
                     RISFRVASKVDSRTILDSNGSESLLGKGDMLYLPAGSARLHRIHGPLVSEDEIVSVCD
                     FWRQQAQAKYNEQLLEAPKDENGKVEGGAEGEGSGGEADDELYQDAVRVVCEMGRAST
                     STLQRRLRIGYGRAAHLI"
BASE COUNT          556 a          933 c          703 g          646 t
ORIGIN      
        1 aatcagatgc gccgcgcgcc cgtaaccgat gcgcaagcgc cgctgcagag tcgaagttga
       61 agcacgaccc atctcgcaca ccacccgcac tgcgtcctgg taaagttcat cgtcggcctc
      121 ccctccgctg ccttcgccct ccgcgccgcc ttccaccttt ccattttcat ccttgggcgc
      181 ttccagcaat tgctcgttgt acttcgcctg tgcttgctgc cgccagaaat cgcacacact
      241 gacaatttcg tcttcactca ccagtggccc gtgaatgcgg tgcagccgcg ccgagcccgc
      301 cggcaggtac agcatgtcgc ccttgcccag cagcgattcc gacccattcg aatccagaat
      361 ggtgcgcgaa tccactttgc tcgccactcg gaacgaaatc cgggcaggga aatttgcttt
      421 aatcaaaccg gtaatcacgt ccacactcgg acgctgcgtc gccagaatca ggtggatacc
      481 cactgcgcgc gccatctgcg ccaatcgcgt gatggactct tccacgttgt tcgtatccac
      541 catcatcaag tcggccaact cgtcgatgat gatcaccagg taaggaagcg gcttgtgctc
      601 gctgtcctcc acattttcaa acagggaaag cgattgctgc ttctgaaacg ttcggttgta
      661 ctggtcaatg ttgcgcacgc cgcgctgagc cagtagcttc agccggcttt ccatttcgcg
      721 tgtcgcgttg cgaagcacat tcgaagcaac cttcgggtcc gttactaccg gcgcgatcag
      781 gtgcggaatc ccctcataca aattcaattc cagacgcttc ggatcgacca gcaccatttt
      841 cacatcatcg ggagtcgcct tatacaggat cgacatcatc aaggagttga tgaagacgct
      901 cttgccggta cccgtcgagc cggcgatcag caggtgcggc atgctggcca gatccgtcac
      961 gcgaatgcgc ccgtgcaggt cgcgccccat tgccagcgtc agtttgctcg gcgaacccac
     1021 aaactcctgc gcctcaataa tttcccgcag cgcgattgtc tgccggttca gatttggcac
     1081 ttcgatgccg atcgtcgatt ttcccggaat gcgttcgatc agaatagact cagcctgcag
     1141 cgccaggcac aagtcctcag tcagtccgat aatccggctg tacttgatgc ccgcttccgg
     1201 tttgaactca aacgttgtca ccaccgggcc aggattgatc tgcgttatgc ggccctgcac
     1261 gtcaaactcc aggcactttt cttcaatcgc ccgtgcgcgt tccttcagtt catcttcatc
     1321 cagcttctgc gaacgctctc cctcccgcag caggctcacc gaaggtagtt tgtagttcgt
     1381 attgcccttg gcaatcttgg gatctccgga cttcttcgct ttttccggct tgtcctggtt
     1441 cagaaagaga atcggcgcct tgtggttctt ttcgtcttcc tcgtcttctt cctccgtggc
     1501 gctctttcct ttacccttga aaatgtcgct ctgatcttgc aggcggattg tctgtggctc
     1561 ctcgtccaac accgctgcac cggcttggga cttgatcggc ttgcgtcctt ccagccgatt
     1621 ggcttccacg cgccgtcgca tgcggtcctg ttcacgccgt tcgcgccagt cgtgccagcg
     1681 cgcctgcgcc cgctgtaaaa cccccagctt gtccattgcg ccaatcggcc cgcgcggccc
     1741 gctggcccag gcatgtgctc gtccaaacga aaatgtcgtg gtcaagaaag tcgcggtgac
     1801 cagcagggcc agcgtgacga tcaacgctcc agccttgttg aaacccgctg tcaaagcacc
     1861 ggatagcagc gccccgatca ttccgcccga cggaatcgct cctcgtacgt cagggaagtg
     1921 aattaacgtc agcaaagcgg gaaggaacaa cagcagcaac gcgtagccga atagcgtggc
     1981 cttctgcgac tctaccggac ggcttcggaa ccatttgatg cccagccaaa gcgccgccat
     2041 cggaatcaaa aacgccgaat aacccagcag ctggaacatt gcatccgcgg tgtacgcgcc
     2101 gaacggccca atcaggttgt gcacctgacc atcccgcgac gacgaaacgt taaacgatgc
     2161 atcgcccggc caatacgtaa acagcgccgc cgccaccaac agtgcgaagg tcaggcacaa
     2221 aaacccagtc agttcattta gccgcttgtt tcccgtaggt gtcagaaagc gcacgcgtaa
     2281 actctcctca gactttgggg aaggctgagt gctgcaagcg gtttggcgct atcacccgcc
     2341 tgcgtggagg aactgcaaaa ccacccaaat cataccaaac gaacccgccg aaatcaccgc
     2401 tagctgcaga gttgtcaggc gaggtgttca attgccaatc accaggtgtc gcaaggaaat
     2461 tcgcggcggc caatttattc gtatccggga tttctcgcct gccacgcctt tatcgtcgta
     2521 tggcttccgc ttctctttct ccttgcccgg gacatgcgtc gcatgtcggt gtcagcgaga
     2581 tcccgtctat ctccgtcgat tcgtccctcg ctgcgtcatc cctggccaat cgatcttggc
     2641 tgttcctgta tgtctagctg atcaatacgc gaatttttcc tcggaccgct gccaactccg
     2701 catcggaaat tcgtccgtta cgcctcgcct ttcgtgctcg ccagtccagg ctcttcacgt
     2761 gatccgcccg tacggcgccg ctgccctggc cgactgcctg ctctcacaca ctattctcgt
     2821 cctcacaggt atcccaat
//
LOCUS       DASTPM010000591         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_857247, whole genome shotgun sequence.
ACCESSION   DASTPM010000591 DASTPM010000000
VERSION     DASTPM010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857247"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFR42_10405"
     CDS             complement(<1..380)
                     /locus_tag="VFR42_10405"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEU0369614.1"
                     /translation="MIRFIYNLFWPIGLLFFLPGYFTKMIRRGGYREKFGQRLGIYDH
                     YLRDRLSNQKSTWLHAVSVGEVNVALKLANALRALEPDLHCVLTTTTTTGFALARKNA
                     PPWIEVMYTPLDYWPIMRRAFSVIR"
     gene            complement(377..961)
                     /gene="rsmD"
                     /locus_tag="VFR42_10410"
     CDS             complement(377..961)
                     /gene="rsmD"
                     /locus_tag="VFR42_10410"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU0369615.1"
                     /translation="MRVIAGSAGGVRLAVPKRGVRPTMDRVKAAIFSSLGDAIVGARV
                     LDLFAGSGALGIEALSRGAASAVFVEDDRQSAEAIEKNLAKTKLNGRVRHQDVFDFLR
                     QVSTAEKFQIIFADPPYEKARRGESSHTQKLLNNESLLQLLEANGIFVLEKRPGEPLR
                     ETKLWRIIRQKAYGATEVLFLNQSAIDNRQSAIT"
     gene            complement(958..>2460)
                     /locus_tag="VFR42_10415"
     CDS             complement(958..>2460)
                     /locus_tag="VFR42_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU0369616.1"
                     /translation="GVNMYVQSQGAQAKIQQELSRSLGVPLKIRSMSVTPWGGLELSG
                     ITIPQMSSEGPIHFLEAKTFRLRVRFLSLFSQRLVIKEVSLIKPKVVWPQNAEGKWRL
                     PSTQAKAARADSTKQSAAPSQPQTDTEFVQTYASSAVTAATGVSPSPQVTVTDLPRRE
                     AKVAVAPEVRRVSIKDGDFTFLDQAGRLLASFNGVDFRTNIRSALALHGDTKVARISL
                     QDRFFLDELRSPLRYEPDVLELSKISARVADGEVNGYFAMQPEAEDSPFTTSVKFRDV
                     LADQLVENAGGPKGMVKGKLEGSFQASGKTGAPDALIGKGEIFLREGRVQQYRLLVLL
                     GQILQIEELQELQLEQAEAKYRLSPGLVTIDELILRSPNIRLTASGTVAFDGTLQLSS
                     QLAINDRIRGQLFRAIRDNFHRIDDPGYSAIEFQVGGTIDRPSTNLVEQLVGRDISSM
                     LNSFLGGKKDRPKKKKKSGDEPTTAAPSRSVSPDQALPEVTATPTQVPSP"
BASE COUNT          510 a          689 c          690 g          571 t
ORIGIN      
        1 cgaatcacag aaaacgcgcg acgcatgatt ggccagtagt ccagcggcgt gtacatgact
       61 tcgatccaag gcggcgcgtt tttgcgcgcc aacgcgaatc ctgtcgtcgt ggtcgtggtc
      121 agcacgcagt gcaaatccgg ttcgagcgcg cgcaacgcat tcgccagttt cagcgccaca
      181 tttacttcgc ctacgctcac ggcgtgcagc catgtcgatt tttgattcga gagccggtcg
      241 cggaggtagt ggtcgtaaat tccgagtcgt tgcccaaatt tttcgcggta gccgccgcgt
      301 cgaatcattt tggtaaagta gcccggcaaa aagaacaaaa gcccgatcgg ccaaaacaag
      361 ttgtagatga agcggatcat gtaattgccg actggcgatt gtcgattgcc gattgattca
      421 aaaacaaaac ttcggtggcg ccgtaggctt tctgccgaat aatgcgccag agcttcgtct
      481 cacgaagagg ttctcccggt cgcttctcca aaacaaaaat gccgttcgcc tccagcaact
      541 gtagtaagct ttcgttgttc aagagcttct gagtatggct gctttcgccg cgccgggctt
      601 tttcataagg cgggtccgca aaaatgattt gaaatttctc ggcggtcgat acttggcgta
      661 gaaaatcaaa aacgtcctgg tgtcgaacgc gcccatttaa tttcgtcttc gcgagatttt
      721 tctcgatcgc ctctgccgat tgccggtcat cttcaacaaa tacggccgac gctgccccgc
      781 ggctgagcgc ttcgatgcca agcgcgccgc ttccggcgaa aagatcgagc acacgcgcgc
      841 cgacgattgc gtcgcccaag ctggaaaaga tggcggcttt cacgcggtcc attgtcggac
      901 gcacaccgcg cttcggaact gccaggcgga ccccgccggc gctgccggcg atcaccctca
      961 tggcgacggg acttgcgtcg gcgttgcagt aacttcaggc agcgcttgat cgggcgaaac
     1021 agagcgcgac ggcgcagcgg tggttggctc gtcacccgac ttctttttct tttttggccg
     1081 gtcctttttt ccgccgagga agctgttgag catgctgctg atatcacggc cgaccagttg
     1141 ctccaccaag tttgtgctag gccggtcaat cgtgccgcct acctgaaact cgatggcaga
     1201 atagcctgga tcgtcgatgc ggtgaaaatt gtcgcggatt gctctgaaaa gctggccgcg
     1261 aattctgtcg ttgatcgcga gttgggagct gagctgcagc gtgccgtcga acgcgactgt
     1321 gccagacgca gtgagccgaa tgttgggcga acgcaagatc agttcgtcta tcgtgaccag
     1381 ccccgggctc aagcgatatt ttgcctctgc ctgctccagc tgaagctcct ggagttcctc
     1441 gatctggaga atctggccga gcaatacgag taatctgtat tgttggacgc ggccttcccg
     1501 caggaaaatt tcgccctttc caataagcgc gtctggcgcg ccagttttgc cggaggcttg
     1561 gaaacttcct tccaacttgc ccttgaccat tcctttgggg ccgccggcat tttcgacgag
     1621 ctggtccgca agcacatcac gaaactttac gctcgtggtg aacggagaat cttccgcttc
     1681 cggctgcatg gcaaagtagc cgttgacctc gccgtctgcg acgcgggcgg aaattttaga
     1741 aagctccagc acgtctggct catagcggag cggagaacgc agttcatcca gaaaaaagcg
     1801 gtcttgcaat gagatcctgg ccacctttgt gtctccgtga agcgcgagcg cgctgcggat
     1861 attggtgcga aaatctaccc cgttaaaact cgcgagcagt ctgcccgcct gatcgagaaa
     1921 agtgaaatcg ccgtccttga tgctgactcg tcgaacttcc ggagcaacgg caacttttgc
     1981 ctcccgtctc ggcagatcgg taactgtgac ctgaggcgaa ggcgaaacac cagttgccgc
     2041 agtcactgcg gaggatgcat acgtttgaac aaattcggtg tctgtttgtg gctgcgaagg
     2101 cgccgcagac tgctttgtcg aatcggcgcg tgccgccttc gcttgcgtac tgggcagacg
     2161 ccattttcct tccgcatttt gcggccagac aacctttggc ttgataagcg aaacttcctt
     2221 gatcacgagc cgttgcgaga aaagcgaaag gaaacgaacg cgcagccgga atgtttttgc
     2281 ctcaagaaaa tgtatcggac cttccgagga catttgcgga atggtgatgc cgctcagttc
     2341 gagcccgccc cagggcgtga cgctcatgct gcgaattttg agggggactc cgaggcttct
     2401 gcttagctcc tgctgaatct ttgcttgtgc gccttgtgat tgcacataca tgttcacccc
//
LOCUS       DASTPM010000592         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_194184, whole genome shotgun sequence.
ACCESSION   DASTPM010000592 DASTPM010000000
VERSION     DASTPM010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194184"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFR42_10420"
     CDS             <1..670
                     /locus_tag="VFR42_10420"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0369617.1"
                     /translation="LAGNTGIDNKTTIVLYGDNNNWFAAYAFWQLKYYGHKSVKLVNG
                     GRKKWLEEKRPLTKDAANVTPATYRVSGTDESLRAFRDDVLAVVQGKKRGQLVDVRSV
                     DEFTGKIIAPPGMTETAQRAGHIPSAANIPWAQAANEDGTFKSSDALAALYQGKGVTG
                     GDEVIAYCRIGERSSHTWFALKYLLGYNNVRNYDGSWTEWGNLIGSPIVNETVSGAKA
                     AGAK"
     gene            724..797
                     /locus_tag="VFR42_10425"
     tRNA            724..797
                     /locus_tag="VFR42_10425"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:756..758,aa:Cys,seq:gca)
     gene            complement(815..2581)
                     /locus_tag="VFR42_10430"
     CDS             complement(815..2581)
                     /locus_tag="VFR42_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0369618.1"
                     /translation="MTHFRLRTQLFIATLLIICGLTGALLLIIRHTVSVETERQVRDG
                     TEASVRAFESVQRQREQQFSRTAAMLADLPTLKAQMQTEHAPTIQDASTSWWKLSGSD
                     LFLLALPDRRVVALHVSRPGWPQEVAESDMKRSIDQGDDDSWWYNNGRLYEVFLRTIT
                     AGTGPASQELGFLAIGYQVDSSVAEHLALVANNQIALATGDRVIASTLPPQDEAAMQR
                     WLSKAGARANSSESEIPLETDHYASSSILLHGALPSPVRCYVMMPLGPVNSFMTRLNR
                     TIFVLGGSAILFGALLFGFVSSRITKPLDNLVSGVRALATGNYTYSIVPEGATEVAEL
                     SQAFAKMRGELLDSQRQQIETERIAALGRAASSISHDLRHYLAAVVANAEFLYEAEEL
                     KLDRSEIYQEIKTASTQMTDLIDSLRELAHQRSAISPEPADLEQTIERAIEAVRARPE
                     FRSRDISLHAGSELEGMFDPRKLERAVFNLILNACEATPDGESRVTVEVQTRLGAFEI
                     RVTDQGTGVPETIRGRAFDPFVSSGKPNGTGLGLAIVSKIVSDHGGTVTIERTSQSGT
                     VMLVKLPRVATPVTSHSDSAIA"
     gene            complement(2613..>3015)
                     /locus_tag="VFR42_10435"
     CDS             complement(2613..>3015)
                     /locus_tag="VFR42_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0369619.1"
                     /translation="VRRAMRRGGPKNQAAASVPAKNVSHEVLEFGDAHVDFTSMEATL
                     AGKGVTLTAQEFKLLKFFASSPGRVLTRDELLNEVWGYQNYPSTRTVDNHILRLRQKL
                     EPDSANPRFFLTIHGAGYKFIPGNTPTNGKS"
BASE COUNT          593 a          976 c          838 g          608 t
ORIGIN      
        1 gctcgccggg aacaccggca tcgacaacaa gacgacgatc gtgctctacg gcgacaacaa
       61 caactggttc gccgcctatg ccttctggca gctcaagtat tacggccaca agagcgtcaa
      121 gctggttaac gggggccgca aaaagtggct cgaagaaaag cgcccgctga ccaaagatgc
      181 cgcgaacgtc acgcccgcaa catatcgcgt atccggcact gacgagtctc tccgcgcctt
      241 ccgtgatgac gtcctcgcag tggtgcaggg gaaaaagcgc ggccagttgg tcgatgtgcg
      301 ctcggtcgac gaattcaccg gcaagatcat cgcccctcca ggcatgaccg aaaccgcgca
      361 gcgcgccggg cacatcccca gcgccgccaa catcccctgg gcgcaagccg ccaatgaaga
      421 tggcaccttc aagtcctcgg acgcgttggc cgctctgtat caaggcaagg gcgtcaccgg
      481 cggcgatgaa gtgatcgcct attgccgcat cggcgagcgc agctcgcaca cctggttcgc
      541 gttgaaatat ttgctgggct ataacaacgt gcgcaattac gacggctcgt ggaccgagtg
      601 gggcaacctg atcggttcgc ccatcgtcaa cgaaaccgtt tctggcgcga aagccgccgg
      661 cgcgaagtaa ccctttttcg agtttgcacg aagccgttcc atcaaacggt tttgtgcgtc
      721 cgaggcgcgg tcgccaagtg gtaaggcaaa ggtctgcaaa accttcatcg gcggttcgat
      781 cccgccccgc gcctccaaat gtcttttgaa acgtttaggc gattgcagaa tcggagtgcg
      841 aggttactgg agtcgccacg cgtggcagct tcaccagcat taccgtgccg ctttgcgacg
      901 tacgctcgat cgtgaccgtg cccccgtggt cgctcacaat cttgctgaca atggccaacc
      961 ccaggcccgt tccattcggt tttcccgagc ttacaaaggg atcgaacgcc cgtccgcgta
     1021 tagtctccgg cactccggtt ccctgatccg ttacccgaat ctcgaaagct cccagccgcg
     1081 tttgcacttc gaccgtgacg cggctttcgc catccggagt ggcttcgcaa gcgttcaaaa
     1141 tcaggttaaa gaccgcgcgt tccagtttgc gcgggtcgaa catgccctcc aactcactac
     1201 ccgcatgcag cgagatgtcg cggctgcgga attcaggccg cgcacgcact gcctcgatcg
     1261 ctcgttcgat ggtttgctcc aggtccgcgg gctctggcga aatggcgctg cgctggtgcg
     1321 ccagttcgcg cagcgagtcg atcaaatcgg tcatctgcgt cgaagcggtc ttgatctcct
     1381 ggtagatttc gctgcggtcc agcttcagct cttccgcctc gtataggaac tctgcattcg
     1441 ccaccaccgc cgccaggtaa tgccgcaaat cgtgtgaaat tgagctggcc gcgcgcccca
     1501 gcgccgcaat ccgttccgtc tcgatctgct gccgctgtga atccagcagc tcgccgcgca
     1561 ttttggcaaa tgcctggctc agctcggcga cttccgtggc tccctctgga acgatcgagt
     1621 acgtgtagtt accggtggcc agtgcgcgca caccggaaac caggttgtcc agcggcttgg
     1681 taattctgct ggacacaaac ccaaacagca gcgcgccaaa taaaatcgcc gacccgccca
     1741 aaacaaaaat cgtgcggttc agtcgggtca taaaactgtt cactggcccc agcggcatca
     1801 tcacgtaaca gcgcacggga gagggaagtg ctccatgcag cagaattgac gaggaggcat
     1861 agtgatccgt ctccagcggt atttcactct cgctcgagtt tgcacgcgcg ccggccttgc
     1921 tcagccaccg ttgcatcgct gcttcgtcct gcggaggcag ggtcgacgca atcactcggt
     1981 ctccggtcgc cagtgcaatc tgattattcg ccaccagcgc cagatgttcc gctacgctgg
     2041 agtccacctg atacccgatg gccaggaagc ccaactcctg cgatgcaggt cccgttccgg
     2101 cggttatggt gcgcagaaat acctcgtaca atcggccgtt gttgtaccac caggaatcat
     2161 cgtcgccctg gtcgatcgat cgcttcatgt cgctttccgc cacttcctgc ggccaccccg
     2221 gcctgctcac atgcagcgcg acgacacgtc ggtccggcaa agccagcagg aacaggtcgc
     2281 tgcccgacag cttccaccag gatgtggagg catcctggat tgtcggcgcg tgctccgtct
     2341 gcatctgcgc cttcagcgta ggcagatcgg ccagcatcgc cgccgtgcgg gaaaactgct
     2401 gctcgcgctg ccgctgcacg ctttcaaacg cgcgcacaga ggcttccgtt ccgtcgcgca
     2461 cctggcgctc ggtttccacg ctcaccgtgt gccggataat caacagcaat gcccccgtca
     2521 agccgcagat aatcagcagc gtggcgataa agagctgggt gcgcagccga aagtgggtca
     2581 ttgccgttcc atcatttcat ccatgcaacg cgtcaagatt ttccgtttgt cggcgtattc
     2641 cccggaataa atttataccc ggcgccatga atggtcagaa aaaatcgcgg atttgcggaa
     2701 tccggctcca gcttttgccg caagcgcaag atgtgattgt ctaccgttcg cgtggacggg
     2761 taattctgat agccccatac ctcgttcaac agttcgtcgc gggtcagcac tcgacccggc
     2821 gaactggcaa agaatttcag cagcttaaac tcctgcgcgg tcagcgtcac ccctttcccg
     2881 gccagcgttg cttccatgct ggtgaaatcc acgtgggcat cgccgaactc cagcacttcg
     2941 tggctgacat tcttggccgg cacgctggcg gcagcctggt tcttcggccc gccgcgccgc
     3001 atcgcccgcc gcacg
//
LOCUS       DASTPM010000593         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_251647, whole genome shotgun sequence.
ACCESSION   DASTPM010000593 DASTPM010000000
VERSION     DASTPM010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251647"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VFR42_10440"
     CDS             complement(<1..1333)
                     /locus_tag="VFR42_10440"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369620.1"
                     /translation="MPIPPTGRCIVSVLAAALVSFFPGSMTGQPAPAPLAQQRSESDS
                     FEALARQATAAREEGKTDDAIRLYEAALQQNPDWTDGLWYLGTLYADAGRYGDAIPAF
                     QKVTTAYPKLGSAWAYLGLCEFETKDFKSSLRHLQNAKQLGFAEVPGVEKVATYHLAL
                     LLNLNGQFEEAWELLASKLGRNQLAEQTKTALALALLRVPLLPDQVDPSKDALIDAAG
                     ETAGDLVEGNFDRAFLGFQQMLKTYPNTPFLHYAYGSALIFRARYAEAEAQLREEVKL
                     TPRSALPFMRLAIIAMKQHHAQDALPMAERAVQLAPESALAHEILGRILAELGKSEQA
                     TKESEAAEKLKPEKPDIDPQVARVYAHASNPGEGTRVSDSAAFDALAREAVAAGQSGR
                     TDDAIRAYESALKVNPQWNKGWADQGALYYAAGKFPQAIAALKNSIGINPKN"
     gene            1417..>2342
                     /locus_tag="VFR42_10445"
     CDS             1417..>2342
                     /locus_tag="VFR42_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEU0369621.1"
                     /translation="MPLLKQNARQSSVSRRLFLRGLISSVALSRFGHSAYAAESPYTF
                     EEVPPSVSGIKWVHTAGKSAEKYLPETTGAGCAFLDYDNDGWMDIYLVNSGKCDFFDP
                     SPSVRNALYRNNRDGTFTDVTEKAGVAGGGYGMGVAVGDYDKDGFPDLFVSQYGRSIL
                     YHNNGNGTFTDVTEKAGVAAPGWASSAVWFDYDNDGLLDLFVGRFGEFSKAKSISCGE
                     VGRRRYCLPRMFQPMPSWLFHNNGDGTFTDVSKESGIASSLGKAWGVVATDINNDGLM
                     DLFVANDTVANFLFANRGKGRFEEIALQADVA"
BASE COUNT          477 a          633 c          697 g          535 t
ORIGIN      
        1 aattcttcgg attgattccg atcgagttct tcagggccgc gatagcctgc gggaacttgc
       61 cagcagcgta gtagagggct ccctggtcgg cccaaccctt gttccactga gggttcacct
      121 tgagcgcact ctcataggcg cggatcgcgt cgtcagttcg gccggactgc cccgctgcca
      181 ccgcttctcg cgcgagggca tcgaaggctg cggaatccga aactcgcgtc ccctctccgg
      241 gatttgaggc gtgggcgtag accctggcga cttgaggatc aatatccggc ttttcgggtt
      301 taagtttctc cgcggcttca gattctttgg ttgcctgttc agactttccg agttcggcca
      361 gaatccgtcc caatatttcg tgggctaggg cagactctgg agcgagttgg acggcacgct
      421 cggccatcgg caaggcgtcc tgcgcgtgat gttgcttcat ggcgatgatt gccaggcgca
      481 tgaagggcag cgcactcctc ggtgtcagct ttacttcctc gcgtaactga gcttctgctt
      541 cagcataccg agcgcgaaaa atcaaagcgg atccgtacgc atagtgaagg aagggcgtat
      601 tcggataggt cttcaacatc tgctgaaatc ccaggaacgc ccgatcgaaa tttccttcga
      661 ccagatctcc cgcggtttcg cctgccgcat caatcaacgc gtccttggag ggatccacct
      721 gatccggcaa cagggggacg cgcaacaacg ccagcgccag cgcagttttg gtttgttctg
      781 ccaactggtt gcgtcccaac ttggacgcca gcagttccca tgcctcttcg aactggccgt
      841 tcaggttgag caacaatgcc aagtgatagg ttgcaacttt ctccacaccg ggaacttcgg
      901 cgaatccgag ctgtttcgca ttctgcagat ggcggagaga actcttgaaa tccttggttt
      961 caaactcaca taaaccaagg tatgcccatg ccgagccgag tttgggatag gccgtcgtta
     1021 ctttctggaa agccggaatg gcgtcgccat agcgacctgc gtcggcgtat agggttccga
     1081 ggtaccacag gccgtcggtc caatcaggat tctgctgcag ggcggcttcg tacaagcgga
     1141 tcgcgtcgtc ggtcttgcct tcttcgcgcg ctgcggtggc ctgccgggcg agcgcctcga
     1201 agctgtcgct ttccgatcgt tgctgtgcca acggcgccgg agcgggctgt ccggtcatcg
     1261 agcccgggaa aaacgaaacc agagcggccg cgagcacaga gacgatgcat cgaccggtag
     1321 gtgggatcgg catgagggac gcactcttgt gggatcttag catgggaatt tcgaggccag
     1381 gtccgtggta aaatcccgcc tactctctaa ggtcggatgc cccttctgaa gcagaatgca
     1441 cggcaatctt ccgtttcccg tcgcctcttc ctccgtggcc tgataagcag cgtggccctc
     1501 agccgattcg gtcactctgc ctacgccgca gagtcacctt ataccttcga ggaggtcccc
     1561 ccctcagtca gcggaattaa atgggtgcac accgcaggca agtcggctga aaaatatctg
     1621 cccgaaacaa cgggagcggg ctgcgcattt ctggattacg acaatgacgg ttggatggat
     1681 atctatctgg tgaatagcgg gaagtgtgat ttttttgatc catcgccgtc cgtgcggaat
     1741 gcgctgtacc gtaataaccg cgatggcacg tttaccgatg tcaccgagaa agccggcgtt
     1801 gcgggtggtg gatatgggat gggagtagct gtaggcgact acgacaagga tggtttccct
     1861 gacctctttg tctcgcagta tgggaggagc attctctatc acaacaatgg caatggaacc
     1921 tttacggacg taaccgaaaa agccggcgtt gccgcgcccg gctgggcttc cagtgcggtg
     1981 tggtttgatt atgacaacga cggtctgctc gacttgttcg tgggacgatt cggagagttc
     2041 agcaaagcta agtcaatttc gtgcggcgaa gtggggagac gtcgctactg tttgcccagg
     2101 atgttccagc ctatgccgag ctggttattt cataacaatg gcgacgggac ttttacggac
     2161 gtcagtaagg aatcagggat cgcgagcagt ttagggaaag catggggtgt ggtggccacg
     2221 gatattaaca acgatggcct gatggactta tttgtggcaa acgatacggt tgccaacttt
     2281 ctttttgcca accgcgggaa ggggcgattc gaagaaatcg cactgcaggc ggacgtcgcc
     2341 ta
//
LOCUS       DASTPM010000594         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_597891, whole genome shotgun sequence.
ACCESSION   DASTPM010000594 DASTPM010000000
VERSION     DASTPM010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597891"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..819
                     /locus_tag="VFR42_10450"
     CDS             61..819
                     /locus_tag="VFR42_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0369622.1"
                     /translation="MSPKESIVQGGENFPPTRWSVVAAARSDDSAERARALDVLFAAY
                     WKPVYKYIRLKFSKPPEEAQDLTQGFFAKLLERKLLSRFTPEKSRLRTYIRLCADSFV
                     LNEIKHAGRQKRGGDAVHLALDFSAAEGELYVQTIDPASIPSEEKFEEFFEKEWIRSL
                     FAAAVRKFRESCASAGKTSAFALFEAYDLAEDDPPSYAHLAQTHGLSVSDVTNRLAWA
                     RREFRSLAKEELRGLCSTDEEFARESRTLFGGKA"
     gene            819..1829
                     /locus_tag="VFR42_10455"
     CDS             819..1829
                     /locus_tag="VFR42_10455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0369623.1"
                     /translation="MDFLSDKALARLRKAETPPDFSATRYTLREFLARGGMGAVYLAQ
                     DQVLDRRVAIKILDSADPDGALAERLNKEARVLAQLEHPGIVPVHDAGTLPDGHTYYV
                     MKFVAGAHLDQFLANVPSLPERLRLFLRICDSVSFAHSRGILHRDLKPSNIMIGAFGE
                     VLVMDWGLAKILEPSVTPDSQPAVRSAALSSSSSTLSARSSAATRDGMAMGTPGFMSP
                     EQAAGKSADLDEGSDIFSLGKLLEFIVRPMANQNSARLSRALQAICDKASAADRTQRY
                     GKVAELADDVSRFLDGAPVSAHRENLWERCLRFYRRYQAAILLIAMYLLMRGLFVIYS
                     RR"
     gene            1944..2300
                     /locus_tag="VFR42_10460"
     CDS             1944..2300
                     /locus_tag="VFR42_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369624.1"
                     /translation="MNKVLLGLLVGAVLGAIDGGTAWFTPAVRSALVGIIFGSTIKGI
                     IAGVAAGIFARKVNSVGLGILFGLAVGFVLAFLVAYLQHGYYSEIIIPGSIVGALVGW
                     ATQRYGAPAPAAASAR"
     gene            2502..>3616
                     /locus_tag="VFR42_10465"
     CDS             2502..>3616
                     /locus_tag="VFR42_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0369625.1"
                     /translation="MNDPAHDRVQFGPFTVDLHTHEVRKDGIRIRLVGQPFDILAVLL
                     SKPGELVTREELRIRLWPGDTFVDFDHGLNAAVNKLRETLGDSAERPRFVETLPRRGY
                     RFIVAVERLDIPSVPSTAEAQPMPLANGSPRAETISGATGAGQQRRSRIWLSPIAAAV
                     ALLLLFAAAAVKTKNGGSREARADSPAPGLRIRPFTGVDQDAAQPAFSPDGKYLAFAR
                     EGMREEESGIFVGAIGGGHQIQLTKSRHDRSPAWSPDGKRIVFSRWSEDHCQIFTVPS
                     EGGAEQRIDTGNVVPRRGELNWSPDGKFIAFNGGDAIHLVNLQTSAVHPLTNPPPTAE
                     DRAPSFSADGRRVMFVRSRGSGFPEEVKTIPADGGEE"
BASE COUNT          747 a         1090 c         1046 g          733 t
ORIGIN      
        1 gctctaagtc ggcaacgctt gcgtcgcaac atttcccttg cgtagactcg ctaacgcaaa
       61 atgtcaccga aagagtccat tgtgcaggga ggcgaaaatt ttccgcccac ccgctggtcg
      121 gtcgtcgcgg cagcgcgcag cgatgattcg gcggagcgtg cccgagctct cgatgttctt
      181 ttcgccgcgt attggaagcc ggtctacaaa tacattcgcc tgaaattttc taagcctccc
      241 gaagaagcgc aagacctgac ccagggattc ttcgcgaaac tgctggaacg caaattgctc
      301 tcgcgattta cgccggaaaa aagccgcctg cgcacctaca ttcgcctgtg cgcggacagc
      361 ttcgtgttga acgaaatcaa gcatgccggc cgtcaaaaac gcggaggtga tgcagtgcac
      421 ctggcgctcg atttttccgc cgctgaaggc gagctgtacg tgcagaccat cgatcccgcc
      481 tccattccgt cagaagaaaa gttcgaggag ttctttgaaa aagaatggat tcgcagcctg
      541 ttcgccgcgg ccgttcgcaa attccgggag tcttgcgctt cggccggcaa aacgtccgcc
      601 ttcgccctct ttgaagccta cgacttagcc gaagacgatc cgccttctta tgcgcacctg
      661 gcacagacac acggcctttc tgtttccgat gtaaccaacc ggctggcttg ggcacgccgc
      721 gaatttcgca gcctggcgaa agaagaattg cgcgggctct gctccacgga cgaagaattc
      781 gcgcgcgagt ccagaacgct ttttggcggg aaagcgtaat ggatttcctc agcgacaaag
      841 cgctggcccg gttgcgcaaa gcggaaacgc cgcccgattt ttccgcaacg cgttacaccc
      901 tgcgcgaatt tttggcacga ggcggcatgg gcgccgtcta tcttgcgcaa gaccaggttc
      961 tcgaccgccg cgtcgccatc aaaattctcg actcggccga tccggacggg gcactcgcag
     1021 aacgcttgaa caaggaagcg cgcgttctgg cgcaactcga acatcccggc atcgtgccgg
     1081 tgcacgacgc tggcacactg ccggacggcc acacttatta tgtgatgaaa tttgtcgcgg
     1141 gcgcgcacct cgatcaattc ctggcgaacg tcccttctct tccggagcgc ctgcggctct
     1201 tcctgcgcat ttgcgattcg gtttccttcg cacactcccg cggcattctg caccgtgatc
     1261 tgaaaccttc caacatcatg atcggcgcgt tcggcgaagt gctggtcatg gactggggct
     1321 tggcaaaaat tctggagccg agtgtcacgc ccgattcgca accggcggtc cggtccgcgg
     1381 cgctttcatc gagttccagc actctgtcgg cgcgaagctc agccgccacc cgagacggca
     1441 tggcgatggg cacgccggga ttcatgtcgc cggagcaggc cgccggcaaa tctgcggact
     1501 tggacgaagg cagcgacatt ttttcgctcg gaaaactgct ggagttcatc gtcaggccga
     1561 tggcaaacca aaattccgct cgtctttctc gcgcattgca agccatctgc gacaaagcct
     1621 cggcggcgga tcgcacccag cgctacggta aagttgccga gctggccgat gacgtttcgc
     1681 gttttctcga cggcgcaccc gtttctgccc accgcgaaaa tctttgggaa cgctgcctgc
     1741 gtttctaccg tcgctaccag gccgccatct tgctgatagc aatgtacttg ttgatgcgcg
     1801 ggctgtttgt gatctatagc cggcgctgaa agctttcccc aagctaaagg aattcgaacc
     1861 atcgttgtaa agaaccccag agcgagcgag aatgcggaca atcaaacccc gcaggttcgc
     1921 aaaaaaattg aggggaggag caaatgaata aagttctgct gggactcctg gtgggcgcgg
     1981 tcttgggcgc cattgacggc ggcaccgcgt ggttcacccc ggcggtgcgg tcagcgcttg
     2041 tgggaattat ctttggctcg accattaagg gaatcatcgc cggcgttgct gcgggaattt
     2101 ttgcgcgcaa ggtgaactcg gtcggtttgg gcattctctt tggattagca gtggggttcg
     2161 tcctggcgtt tctcgtggcg tacctgcagc acggttacta ctccgagatc attattcccg
     2221 gcagcatcgt gggagccctg gtcggctggg ctacccaacg ctatggcgcg ccagcccccg
     2281 cggccgcgtc ggcccgctag gcacaaactg ggctaaagcg gaaggccgca tgcatttccg
     2341 gtcattcctc atccattgct ggtcatccat taactaactg aaataattgc gcttacgatt
     2401 ccggaagcgg gaacaaacaa gctccgccgc gcgtatctca ctgggcaaga caagactgct
     2461 ggccaccgtc ggcagtctaa attccaagag atgcccgaga aatgaacgac ccggcacacg
     2521 atcgcgtcca gtttggtccc tttaccgtcg acctccacac ccacgaagtg cgcaaagacg
     2581 gcattcgaat tcgcctggtc ggccaaccgt tcgacattct ggcggttctg ctcagcaaac
     2641 ccggcgagct ggttacccgc gaggaattaa ggattcggct gtggcccggc gacacctttg
     2701 tggacttcga ccacgggtta aacgccgcgg tcaacaaact aagagagacg cttggcgatt
     2761 ccgccgagag accgcgcttt gtcgaaacgc taccgcgtcg cggttaccgt ttcatcgtgg
     2821 ccgtcgaacg actcgacatt ccgagcgtgc cgagcacggc ggaggcacag ccgatgccac
     2881 tcgcgaatgg ttcgccgaga gcggaaacaa tttccggtgc gacgggcgct ggtcagcagc
     2941 ggcgcagcag gatttggctc agcccgatcg cagccgcggt tgctctcctc ctgcttttcg
     3001 ccgccgctgc cgtaaaaaca aaaaatggcg gatcgcgaga ggcgcgagcc gactccccag
     3061 ccccgggact gcgcatccgg cccttcaccg gtgtggacca agacgcagcg caaccggcgt
     3121 tttccccgga tggaaagtac cttgcgtttg cgcgcgaagg gatgcgcgaa gaagagtccg
     3181 gaatttttgt cggcgcaatc ggaggcggcc atcagattca actgacgaag agccggcatg
     3241 atcgcagccc ggcgtggtcg cccgacggaa agcgcatcgt tttttcgcgc tggagtgaag
     3301 atcactgtca aatcttcacc gtgccatcgg aaggcggcgc agagcagaga atcgacaccg
     3361 gcaatgtggt tcccagacgc ggcgaactga attggtcgcc ggatggaaaa ttcatcgcat
     3421 tcaacggcgg tgacgcgatc catctggtga atctgcaaac gtccgcggtt catccgctga
     3481 cgaatccgcc tccaaccgcc gaagaccgcg ctccatcatt ttccgccgac ggcagacgtg
     3541 tgatgtttgt gcgcagccgc ggctccggtt tccccgaaga ggtcaagacg attcccgcgg
     3601 atggcggaga agaaat
//
LOCUS       DASTPM010000595         4215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_624258, whole genome shotgun sequence.
ACCESSION   DASTPM010000595 DASTPM010000000
VERSION     DASTPM010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624258"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(440..1261)
                     /locus_tag="VFR42_10470"
     CDS             complement(440..1261)
                     /locus_tag="VFR42_10470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0369626.1"
                     /translation="MTQKPAAEKADLYNSAYGNYGAEVYRQIRVETYGEDLGQTSWVT
                     TDESAEIMRLLALTRDSHVLEIGSGSGRYALQVASTTGCRVLGVDINEPGVQNANQLA
                     VAENLAERVKFEKCDASQPLRFSDASFDAVFSNDVLCHIPGRDKLLRELRRVLKPGGR
                     LLFSDALVIGGVISHQEIAARSSIGYFLFSPPGENERMLREVGFVLVEARDATANASQ
                     ISKRWHDARAKRAEALTRIEGQENFEGLQRFLATVHRLTSERRLRRLLYCAEQPC"
     gene            complement(1258..1854)
                     /locus_tag="VFR42_10475"
     CDS             complement(1258..1854)
                     /locus_tag="VFR42_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEU0369627.1"
                     /translation="MAVCLFTAENIWIDPWVARKWHHKLPSFVPEALGGAWFLILMGL
                     AITVILLVVCQVLLMRDSQIAVWKKAMTGVLVVVAALLSCGWLVATGGISFAGRRSAE
                     AASPPQKRSVVLRWQASKSPLVRYNVYRGPWAGIYPDKLNSAPIDGTTFTDTTAVSGQ
                     TYAYIVRAINSKGEESQDSNETIVTIPRNANSATRSTP"
     gene            complement(2110..2982)
                     /locus_tag="VFR42_10480"
     CDS             complement(2110..2982)
                     /locus_tag="VFR42_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEU0369628.1"
                     /translation="MTATPATLKTGEQTVEIKLAHSPDSDDAFMFYALATHKLQTPGY
                     KYTHVLSDIQTLNEAALTETYDVTAVSFAAYPSIRDKYVLLDCGASFGEGYGPIVVAA
                     KSMKKEDIKGKRIAVPGLKTSAYLTLKLFEPHFEAVVMPFDKILDAVQDELVEAGLLI
                     HEGQLLFPQLGLYKVLDLGVWWQEQTGLPLPLGGNAVRRSLGPELGRLIAKTIRDSVE
                     YGLEHREDALKYAMQFARDMETELADQFVGMYVNKWTLGYGEKGRQAVQELITRGTKA
                     GILPGPDSVEFLES"
     gene            3394..4134
                     /locus_tag="VFR42_10485"
     CDS             3394..4134
                     /locus_tag="VFR42_10485"
                     /inference="COORDINATES: protein motif:HMM:NF025428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HEU0369629.1"
                     /translation="MGIAKRELEAKLNTDALTEHPHRFGTTVYSFSVAGKDSVSTWRR
                     LRQSAPGLGFWPVIFGEPKNVERICEVFATPDAESPAEAIRMASEVDVQEFFRGRAQE
                     FSGEGSRGEWPKDVEGMTDFTIPRKTLQPDQFHETVVIGLVPASHSWELPAVLSFGGW
                     NDCPFPTDQAAVLRYWNEIYGAELMGLSGDVMELEAARKPSTKEEALKLAQEQYWFCY
                     DIVEQGVGTIENLAGGLMASSVWYFWWD"
BASE COUNT          888 a         1158 c         1263 g          906 t
ORIGIN      
        1 tcgcacacca ggacactgcc acgcagctca ctgcccatcc caaaaatatt tgttttttca
       61 tttccagttc acgttacgaa aagttgccgc aatcggtcaa gggcgcgcat attcgggggg
      121 cacgcgtatt ctggcgccag gaagccaaac tgactgtaag cacaacgaca accatgagga
      181 ttcccgcggc caaccaacac aaacgaattt gtataagcga ggctgttcgg tgcaattgag
      241 cgggccgccg cggctgtacc aaggtcgttt ttctctgtac ctccggccca gccacacaaa
      301 ataggtgaga agcagtccgg cccagccgac cgataggaaa attccacgag agagcagaaa
      361 aaagaagaag aagagacagt acgccgatag aaaacaaggt tgtctccgtt tgggccctat
      421 tcgataaatt cgttttcttt cagcatggct gttcggcgca atatagcagc cggcgaagac
      481 gcctttcgct cgtaagcctg tgcacggtcg caagaaaccg ctgcaagccc tcaaagttct
      541 cttgaccctc aattctggtc agcgcctcgg ccctctttgc cctcgcgtcg tgccagcgtt
      601 tcgaaatctg gctggcgttc gccgtagcat cccgggcttc caccagcaca aatcccactt
      661 cccgcaacat gcgttcattt tccccaggcg gactgaaaag aaaatatccg attgagctcc
      721 gcgccgcgat ctcctgatgc gaaatcactc cgccaatcac cagcgcatcg ctgaagagga
      781 gccgccctcc cggtttcagc acgcggcgca attcccgcaa cagtttgtcg cgcccgggaa
      841 tgtgacacag cacatcgttc gagaaaacag catcaaacga agcatcgcta aatcgcagcg
      901 gctgggatgc atcgcacttc tcaaacttca ctcgctctgc caaattctcc gccaccgcca
      961 actgattcgc gttctgtacg cctggctcgt tgatgtctac tccaagcacg cggcaacccg
     1021 ttgtactcgc tacttgcaga gcataccgcc ccgacccgct tccgatttcc agtacgtgcg
     1081 aatcacgcgt cagcgccagc aggcgcatga tttctgccga ctcgtccgtc gtcacccagc
     1141 tcgtctgccc aaggtcttcg ccgtaagtct ccacacgaat ctgccggtac acctccgcgc
     1201 cataatttcc atacgcgctg ttgtacaggt ccgctttttc tgcagctggc ttttgcgtca
     1261 cggcgttgat ctcgtcgcgc tgttcgcgtt tcgcggaatg gtgacaattg tttcgttgga
     1321 gtcttggctt tcttcaccct tgctgttgat cgcgcgcacg atataggcgt acgtctgccc
     1381 gctgaccgcc gtcgtgtcgg tgaaggtcgt tccatcaatc ggcgcgctgt tcaacttgtc
     1441 gggataaatt ccagcccacg gtccccggta cacgttgtaa cggacaagcg gactcttgct
     1501 ggcctgccag cgcaatacca cgctgcgctt ttgcggggga ctcgccgctt cggcgctcct
     1561 tcgcccggcg aagctgattc cgcccgtcgc gacaagccat ccgcaagaca aaagtgccgc
     1621 cacaacaacc agaacgccgg tcatcgcttt cttccaaacc gcgatctgcg aatcgcgcat
     1681 gagcaacacc tggcacacca ctagcaaaat caccgtaatc gccaggccca tcaaaatcaa
     1741 gaaccacgcg ccgcccagcg cttccggcac gaaggacggc aatttgtggt gccactttct
     1801 cgccacccac ggatcaatcc agatattctc cgcggtgaac aggcacaccg ccacggaaag
     1861 tgcccacgcg attctggcaa ttcgggaact ggcttctgcc atgttgtcgc tattatctac
     1921 gatttcgccg cccgcagaga cgagccgctg ctcctgctac gcttttgcgg cggtgccggg
     1981 cgaagcttca gcgggacaag tgattctgga tgcaggtccg ttgttgctga atcttgaggt
     2041 cgggctggaa gccctgttcc tttcccgttt cgcagaggcg cccgcttcag caaacggccg
     2101 ctacaactat tacgactcga ggaactcgac ggagtcgggg ccggggagga tgcccgcttt
     2161 ggtgccgcga gtgatcagtt cctggacggc ttgccggcct ttttcgccgt agccgagggt
     2221 ccatttattg acgtacatgc cgacgaattg atcggcgagt tcggtttcca tgtcgcgcgc
     2281 gaattgcatg gcgtacttca aagcgtcttc gcggtgctcg aggccgtatt cgacggaatc
     2341 gcgaatggtt ttggcgatca agcggccgag ctcgggacca agcgagcggc gaacggcatt
     2401 gccgccgagc gggagaggaa gtccagtttg ctcctgccac cagacgccga gatcgagaac
     2461 cttgtacaaa cccaattgcg ggaacagcag ctggccttcg tgaatcagca ggccggcttc
     2521 gacgagttcg tcctggacgg cgtcgagaat tttgtcgaag ggcatgacca cggcttcaaa
     2581 atggggctcg aacagtttca aggtaaggta ggcggaagtc ttgagtccgg ggacggcgat
     2641 gcgtttgccc ttgatgtctt cctttttcat ggacttggcg gcaaccacga tggggccata
     2701 gccttcgcca aagctggcgc cgcaatcgag caggacgtat ttgtcgcgaa tggatgggta
     2761 ggcggcgaag ctgaccgcag tgacgtcgta cgtttcggtg agcgcggctt cgttgagggt
     2821 ttgaatatcg gagagcacgt gagtgtattt gtagccgggc gtttggagct tgtgcgtggc
     2881 cagggcgtaa aacatgaaag catcgtcgga atcgggggaa tgcgcgagct tgatctcgac
     2941 agtttgttca ccagttttaa gagttgcggg tgttgcggtc atagcgggtc gtgcttcctt
     3001 tgtgtcggcg gccacgaatc gattctgcta cccgtgtgcg gtctgggacc gagaaggctc
     3061 tccccgagag caacggcggg acgaaaatga gaagagcaag gcgacgtacg tggcgcagtg
     3121 ccgggtctgg ttgtttgcgg gaagaaggtt agcatgggcg gggccggagt ggcgagtgga
     3181 agaaagatgc ccgcgaagga cctggctgat ggttgaaagt gggggtcgtg gctccgggca
     3241 caagggcaac cgacgggggc ccacccggtg tttttcgtaa gtacgagaag gaaaggagtt
     3301 agcggcggtc gtttttctac cgggactaca atggcggtcc agcgtcgaaa gttggaagtc
     3361 aagaatagtg attggggaaa ggggcagata gagatgggga ttgccaaacg tgagctggaa
     3421 gcgaagttga acacagatgc gttgaccgag catccgcacc gcttcggtac gacggtgtac
     3481 tcgttctcgg tggcgggaaa ggattcggtt agcacgtggc ggcggctgcg ccagtccgcg
     3541 ccgggtttgg ggttctggcc ggtgattttt ggcgagccga agaacgtgga gcggatttgc
     3601 gaagtctttg ccacgccgga cgcggaaagt cctgcggagg caatccgaat ggcaagtgaa
     3661 gtggatgtgc aagagttctt tcgcgggcgt gcccaggagt ttagtggaga aggttcgcgc
     3721 ggcgagtggc cgaaagatgt ggagggaatg acggatttca cgattccgcg caagactctg
     3781 cagccggatc aatttcacga aaccgtggtc attggacttg tcccggcaag ccactcgtgg
     3841 gaactgcctg ccgtgctttc cttcggcggc tggaacgatt gccccttccc cacggatcaa
     3901 gcagcggtgc tgcgctattg gaacgagatt tacggggcgg aacttatggg cctgtccggc
     3961 gacgtgatgg agctggaagc ggcgaggaaa ccgtcgacga aggaagaggc gctgaaactg
     4021 gcgcaagagc aatattggtt ttgctacgac attgtggaac agggagtcgg gacgattgag
     4081 aatctggcag gtggattgat ggcgtcgagt gtttggtatt tttggtggga ttgatctacc
     4141 gcaagaacag ttcttggaaa cctggtttga caaattttga aatacccagc tttccgtgca
     4201 ggtgactcgc gccac
//
LOCUS       DASTPM010000596         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1405338, whole genome shotgun sequence.
ACCESSION   DASTPM010000596 DASTPM010000000
VERSION     DASTPM010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405338"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFR42_10490"
     CDS             complement(<1..722)
                     /locus_tag="VFR42_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU0369630.1"
                     /translation="MKLLLIDGHYYVYRSFFAIPNLSNSRGEPTNAIFGFTKTLRLML
                     KHLQPQLGAVFWDEGLPQRRVELQPAYKETRKEMPRPMIPQLDFIRSTITPLLGFTNV
                     ALPDTEADDLMGCYSIAACRDDIEVVLATNDKDLYQLVGPCVKVYTTAKADLASPKDA
                     FALLGEEEVTTKWGVPPTLIGDVLAIAGDTVDNIPGVGIGRKTAAGLIREHGGLEALL
                     ANLASVKSLKTREKLQNGREQIL"
     gene            1021..1959
                     /locus_tag="VFR42_10495"
     CDS             1021..1959
                     /locus_tag="VFR42_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369631.1"
                     /translation="MVRRRTFSEIIRDSFRIYKSAFLPFFVLTLLIAVPTLALELTSP
                     AYGIFPQAEGGGLTRARVIALIAFTALVVDWPVFLAGIQIATVDVLEGRKVRLGELLR
                     RAANFFPRFARLSLIVYGSYFLCTAIPVAIILSLASDNPTILSLVLALLILGVQVIMV
                     ARLWVNFLFWQQSAYISNLEGLDALRESKLLARSKRRPRKSSRPLWRGALLVSLWILI
                     VLGLSAGAEIPLILPKLQSLSTSSPEEIMKMWQDLNTPKVADVMLIASMAISSLVRAL
                     LRPILGIAFVLLYLDTRADFSESELAPPDRQSDRLS"
BASE COUNT          461 a          627 c          639 g          565 t
ORIGIN      
        1 aggatttgct cacgcccgtt ttgcaatttc tctcgcgttt taaggctctt gaccgaagca
       61 agattggcca gcaaggcctc cagcccgcca tgctcacgaa tcaggcccgc cgcagtcttt
      121 cgtccaattc cgaccccggg gatgttatcg acggtatcgc ctgcaatcgc cagcacatcg
      181 ccgatcaatg tcggcggcac gccccatttc gtggtgactt cttcctctcc gagcaaggcg
      241 aaggcgtctt tcggcgaagc caggtcggcc ttggcggtgg tataaacttt gacgcacggg
      301 ccaaccagct gataaaggtc tttgtcgttt gtcgccagca cgacctcgat atcgtcccta
      361 cacgccgcga ttgaataaca ccccatcaaa tcgtccgcct ctgtatcggg cagtgcgacg
      421 ttggtgaatc ccagcaatgg cgtaattgtg ctccgaatga agtccaactg cggaatcatt
      481 ggcctgggca tctctttgcg ggtttccttg taggccggct gcagctccac tcggcgctgc
      541 ggtaaacctt cgtcccaaaa caccgcccca agttgcggct gcaaatgttt cagcatcagc
      601 cgcagcgttt tggtgaaacc gaaaatcgcg ttcgtcggtt cgccgcggga gttgctcagg
      661 ttcgggatcg cgaaaaatga gcggtaaaca taatagtgcc cgtcgatcaa aagcagtttc
      721 atgaatgccg agaattattt ccgtcattac gagcgaaagt cgagggatcc ctctacctgg
      781 ttggggtgac agcaatgccg tttcgcgata agtctgccta tggcggatcg atggctcgtg
      841 cgggtaggtg aaaaggaata cgggccggtt gatctcgata cgcttggcga ctggaaacgg
      901 gaaggccgct tgctgccgac aaacgaggtc cgccgtgagt cggagagcga ttggaccagg
      961 gcgactaccg ttccggggct cttcggcccg ccaccgctgc cagcagcgac agggcatcca
     1021 ttggtgcggc ggcgcacctt tagcgaaatc attcgggata gttttcgcat ctacaagtca
     1081 gcgtttctgc cgtttttcgt tctgactctg ttgatcgcgg tcccaacact tgcgctcgag
     1141 cttactagtc cagcatatgg gattttcccg caggcggaag ggggtgggct gacgcgagct
     1201 agggtcatcg cattgatcgc ttttaccgcg ctggtggtgg attggccggt ctttctcgcg
     1261 ggtattcaga tcgccaccgt cgatgtcttg gaaggacgta aagtacgcct gggcgaactg
     1321 ctccggcggg cggccaattt ttttccgcgg tttgcacggc tcagtcttat tgtttacggg
     1381 agttattttc tttgtaccgc cattccggtc gccattatcc taagtcttgc cagcgacaat
     1441 cccacgattc tctcccttgt tctggcgctc ctcattttgg gcgtgcaggt gataatggtc
     1501 gcgcgtttgt gggtgaactt tctcttctgg cagcagtctg cttacatttc gaaccttgaa
     1561 ggcctcgacg cgttacggga aagcaaacta ctggcccgca gcaagcgccg gccgcgcaag
     1621 tcgagccgcc ctctttggcg cggcgctttg ctcgtctccc tctggatcct gatcgtgctt
     1681 ggattgagcg ccggggcgga aattcctttg atcttgccga aattgcagag cctttcgact
     1741 tcgtctccgg aagaaataat gaaaatgtgg caggatctga acacgccaaa ggttgccgat
     1801 gtcatgttaa tcgccagcat ggccatcagt agcttagtgc gggcactgct gcggccaatt
     1861 ctcggcattg cgtttgttct gctttatttg gatacgcgcg ctgatttcag cgagagcgag
     1921 ttggctccgc cggaccgtca gtctgataga ctttcctaaa tgacccgagc ccgtcagcta
     1981 cgggaaagtg ccactcagca tgaacgactc ttatggcgtc accctcgcaa tcgacaagtt
     2041 cagggctgga aatttcgccg acaacatccg gtggatcgct acatattgat ttttactgcc
     2101 ctgaagcccg cctcgcggtt gagctcgacg gtggaggcca caatttcgcg aacagggaat
     2161 cgttagatca ggaacggacg agatttcttg cgagcaagaa tattgttgtt catcgatatt
     2221 ggaaccatca gatcaaccag caaactggac agtgttcttg agacgatttt gtcggcgctt
     2281 aaacaacgag ta
//
LOCUS       DASTPM010000597         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1088061, whole genome shotgun sequence.
ACCESSION   DASTPM010000597 DASTPM010000000
VERSION     DASTPM010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1088061"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1561)
                     /locus_tag="VFR42_10500"
     CDS             complement(<1..1561)
                     /locus_tag="VFR42_10500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEU0369632.1"
                     /translation="MSQQDERMAELRELFFESAQELLQALNDEALKLEKNPGDSEIVR
                     SLRRIVHTLKGDAAACGYRELSEVAHELEDALALESAAAHATLAEVAFTAADLFSAML
                     AAYRKHAKLPTRDPLRKMVKKLTEEPGSKRSAKKKAVVTAKTLGWTEYEKLAVQKAQE
                     GGDFVYHVQAHIDPHCAMPIAGRQLLQNALATLGDVLAARPEAGSPTATKQVEFLVAT
                     EKTGEQVCAKCQIPTIVANVKAELLPGEVAAAAERPAKRVVKEAAVAVTAQPTLEHDV
                     VDAHQAHEDSAETVTPAPTTLAENILRVDAERIDSVLNLVGELIIGKSMLQQALNELA
                     RTYPKDQLRGKFSDAMAFQARVLNDLQRSVMKIRMVPVEQLFRRFPRMVRDVARQCGK
                     DVELVVSGQDTDLDKSILDAIAEPLTHLVRNAIGHGLESPEERKTAGKSIRGTLHLGA
                     YHQGNQVIVEVRDDGRGMDGERIKTKAIELGLVTREEAGRLTEAETLNFVFRPGFSTA
                     EQVTEVSGRGVG"
     gene            complement(1558..2469)
                     /locus_tag="VFR42_10505"
     CDS             complement(1558..2469)
                     /locus_tag="VFR42_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEU0369633.1"
                     /translation="MGTSGAAPAPALTEAELKLLQALVYQECGMHFDERRTHFLQDRL
                     LRRLRECQVDSFYSYYRLLISDAGRSELALLLENLTVNETSFFRNRAQLDLFHKYVLE
                     DLLHRKQERRDFALRFWSAGCSTGQEAYTMAMQVADALAYYYMRNPLPVEAPTPKPLI
                     PPPWKVEILASDISYSVLRAGQEGTYSENQMSTVDYSYRLRYFDKIGDRYAVKKALKE
                     VVHFDFHNLKTEYLPQRNDVIFCRNVMMYFDEAEQKRLINKFYRCLNPDGYLFVGHAE
                     SLLGLTEKFQMVHRNSGTAYQRVEGML"
     gene            complement(2488..3006)
                     /locus_tag="VFR42_10510"
     CDS             complement(2488..3006)
                     /locus_tag="VFR42_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU0369634.1"
                     /translation="MERHTNRELQIVGFQVGNETYGVPITALHEIVRVPDITAVPDAP
                     EYLEGVINLRGKIVSVIDLRKRFGIKETGTSRRNRILVVEFNGRLSGLIVDSASEVLK
                     IAAADIEPPPAVLQEGGLSCVTGLGKYKGRLIMLLDTTKLLEAGSLKAQQAADGKAAA
                     NSKPEARGASAR"
     gene            complement(3015..>3236)
                     /locus_tag="VFR42_10515"
     CDS             complement(3015..>3236)
                     /locus_tag="VFR42_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369635.1"
                     /translation="QAVVKAMERMRELIQQSTSGSTELAASAEQMSKMSRELLDALER
                     VAGRTGDVEHTRSNDKSSSKFARRATAGA"
BASE COUNT          536 a         1037 c          941 g          722 t
ORIGIN      
        1 tacccacgcc gcgccccgac acttccgtga cctgttccgc cgtgctgaat ccggggcgga
       61 atacgaagtt cagagtctcg gcttccgtga ggcggccagc ttcctctctc gtgaccagtc
      121 ccaattcgat ggccttcgtc ttgatccgtt ccccatccat gccgcgtcca tcgtcccgca
      181 cttcgacgat cacctggttg ccctggtggt acgcgcccag gtgaagtgtc ccccgtatcg
      241 atttgccagc cgtcttgcgc tcttctggag attccagacc gtggccgatc gcattgcgta
      301 ccagatgggt cagtggttcg gcgatagcgt ccaggatgct cttgtcgagg tccgtgtctt
      361 gtccgctgac caccagttcg acatccttgc cgcactgtcg cgccacgtcg cgcaccatgc
      421 gtgggaaacg ccggaacaac tgctccaccg gcaccatccg aatcttcatg accgaccgct
      481 gtagatcgtt cagcacgcgt gcctgaaagg ccatcgcatc gctgaatttc ccgcgcaact
      541 ggtccttggg ataagtccgg gcgagttcgt tcagcgcctg ctgcagcatg gacttgccga
      601 taatcaactc gccgaccagg ttcagcacgc tgtcaatgcg ctcggcatcc actcgcaaaa
      661 tgttttcggc caaagtggtg ggggccgggg tgacggtttc cgccgagtct tcgtgtgctt
      721 ggtgcgcatc cacaacatca tgttcaagcg taggctgcgc agtaacagca acggccgctt
      781 ccttgacgac gcgcttggcc ggacgttccg cagccgcggc gacctctccc ggaagcaatt
      841 cggcctttac atttgccacg atggtgggga tctggcattt cgcgcaaact tgttcgcccg
      901 tcttctccgt ggccaccagg aattccacct gcttggttgc ggtcggggaa cccgcctccg
      961 gacgcgcggc cagaacgtct cccagcgtcg cgagcgcatt ctgcagcagt tggcgtccgg
     1021 cgatcggcat ggcacagtgc ggatcgatgt gggcctgcac gtggtaaacg aaatccccgc
     1081 cctcctgggc tttctggacg gcgagtttct cgtattcggt ccagcctagc gttttggccg
     1141 tgaccacagc cttcttcttc gcgctccgct tgctgcccgg ttcctcagtg agtttcttga
     1201 ccattttccg caacggatcg cgggtgggta acttggcatg cttgcgatac gctgcgagca
     1261 tcgcggaaaa caggtccgcg gcggtaaacg ccacttccgc cagcgtcgcg tgtgcggccg
     1321 cagattcgag cgccagcgcg tcttccagtt cgtgtgcgac ttcgctgagc tcgcggtatc
     1381 cgcaagccgc cgcgtcgccc ttcagcgtgt gcacgatgcg gcgcaggctg cgcacaatct
     1441 cggagtcgcc gggattcttt tcgagcttca gcgcttcatc gttcagagcc tgcagcaatt
     1501 cctgcgcgct ctcaaagaag agttcccgca gttccgccat gcgctcgtct tgctggctca
     1561 tagcattccc tcgacgcgct ggtaggcggt accgctgttg cggtgcacca tctggaattt
     1621 ttccgtgaga ccgagcaggc tttcggcgtg cccaacgaac agatacccat ccgggttcag
     1681 gcagcggtag aacttgttga tcagccgttt ctgctcagct tcgtcgaagt acatcatgac
     1741 gttccggcag aaaatgacgt cgttccgttg cggaaggtac tcggtcttca gattgtgaaa
     1801 gtcgaagtgc accacttcct tgagagcctt cttgaccgcg tatcggtcgc cgatcttgtc
     1861 gaagtagcgc agccggtagc tgtaatccac cgtcgacatc tggttctcgc tgtacgttcc
     1921 ctcttgtccg gcgcgcagca ccgaatagct gatgtcgctg gccagaattt ccactttcca
     1981 cggcggcgga atcagcggct tgggtgtggg ggcttccacg ggtaacggat tccgcatgta
     2041 gtagtaggca agcgcgtccg ccacctgcat ggccatggtg tacgcctcct gcccggtcga
     2101 gcagccggcg ctccagaagc gcaacgcgaa atcgcggcgt tcctgcttgc ggtgcagcag
     2161 gtcttccagc acatatttgt ggaacagatc cagttgcgcg cggttccgga aaaaactggt
     2221 ttcgttgacc gtcaggtttt cgaggagtag cgccagttca ctgcgccccg catcgctgat
     2281 cagcaaccgg tagtagctgt agaaggaatc gacctggcat tcccggagac ggcgcagcag
     2341 gcggtcctgc aggaaatgcg tgcggcgctc gtcgaagtgc atgccgcact cctggtatac
     2401 gagcgcctgc agaagcttca gctctgcttc agtgagtgcg ggtgccgggg ctgcgcctga
     2461 tgttcccatg ggtctggttc gctgtggcta acgggccgac gcgccgcggg cctccggttt
     2521 cgaatttgcc gctgctttgc cgtccgccgc ttgctgcgct ttcaatgagc ccgcttccaa
     2581 aagcttggtg gtatcgagca gcatgatcag ccgtcccttg tattttccga gtccagtgac
     2641 gcagctcaag ccaccctcct gtagaacggc gggcggcggc tcaatgtcgg cggccgcaat
     2701 cttcagcact tcagatgcgg agtccacaat caacccggac agacgcccgt tgaactcgac
     2761 caccaggatg cggtttcgtc ggctcgttcc ggtttccttg atgccaaagc gcttgcgcag
     2821 atcgatcacc gacacgatct ttccgcgcag attgatcacg ccttccaggt actccggcgc
     2881 atccggtacg gccgtgatgt cgggcacgcg cacgatctcg tgcaacgccg tgatgggcac
     2941 gccataggtc tcgttgccca cctgaaagcc cacgatctgc agttcgcggt tggtgtggcg
     3001 ctccatgctc atccctaggc tcccgcggtt gcgcgacgtg cgaacttgct cgaactcttg
     3061 tcgttgctgc gggtgtgctc cacgtcgccg gtgcgacccg caacccgctc cagcgcgtcc
     3121 agcagttcgc gcgacatctt cgacatctgc tcggccgaag ccgccaactc ggtcgatccc
     3181 gacgtcgact gctggatcag ctcgcgcatg cgctccatgg ccttcaccac tgcctg
//
LOCUS       DASTPM010000598         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1318861, whole genome shotgun sequence.
ACCESSION   DASTPM010000598 DASTPM010000000
VERSION     DASTPM010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318861"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..398)
                     /locus_tag="VFR42_10520"
     CDS             complement(9..398)
                     /locus_tag="VFR42_10520"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0369636.1"
                     /translation="MSLLHFRCKERRRTQRVSLTVPLVLNGQNEDSEKFSIRAKTNAV
                     SQHGAQIETEHPLVVGQVLLLINENSARKVEAHVASIQRKRDGKTYVGLEFLSTDINF
                     WNMTFPLPGAKPLRRALSKVGQNKVTA"
     gene            796..1371
                     /locus_tag="VFR42_10525"
     CDS             796..1371
                     /locus_tag="VFR42_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369637.1"
                     /translation="MKPFALLLLLCSIACAPRSSAQDHFAVGVYADYFRLSQTDTNSA
                     GLGGRLGFGIAPHVMLEAEVNYDFGVTFTETFANGGSLVFQRTDMRLLHGLFGPKVSL
                     GHSNFHFFVTAKGGFLDTMLDSRPATLGTFLSSVDNLRSQNISAVFYPGGGVEGRIGP
                     IGLRLDAGDEMYFNSGTHHNLRLAFGPYVRF"
     gene            1639..1992
                     /locus_tag="VFR42_10530"
     CDS             1639..1992
                     /locus_tag="VFR42_10530"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HEU0369638.1"
                     /translation="MKSFFANPLACAWLAGALTLTVPSLVAAQEPSQSPQDQGQSQQR
                     DNGQATVAENSFSGRITKANNGKFRLEDRSTRTSFALDNQKLAKKYEGKNVVVTGTLD
                     EANNILHVRKIELAV"
     gene            2257..3624
                     /locus_tag="VFR42_10535"
     CDS             2257..3624
                     /locus_tag="VFR42_10535"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEU0369639.1"
                     /translation="MTTTVLRIPALAGPLCYIAKLALRVRLHVNSQLQNDPRRALNPE
                     KVKRVEVYWHAVTYKTLIAYGLILALVIFSAVYFVTPGFYAAVIRKVSSAVGGTDTEV
                     TPLANTQAKFVNLDGRVQVKKVSSVQWVDADYRTTLDKGDLIQTGSDGAARITFADTT
                     SYTIKADTLVTVEENNVTRDQATTAVRINTGAVDLSTASSPNSHTAVSVEDTVAQIRP
                     NSRATVKSDPQSKEAEFTIASGSAEVQRGGEKVELGQWQRANVRSGSPISTSDVLAPP
                     DLAQPLNLAPIITQVPKTTPVHFEWHLVKDAVTYNLRISTTAMFTKLVKDLKLAGTSA
                     DITGLDAGDYFWNVIAVDGKKQTSEISETFKFTIVVQGKSQEMVLEIGGTQLHGRVAE
                     VFGRTEPGAALIINGQPVPNIGSDGSFRYFTEPLDPGEHTIVIVGQNRRGGTAKQQVT
                     IVVPK"
BASE COUNT          800 a         1066 c         1038 g          754 t
ORIGIN      
        1 aaaaccgctc aggcggtgac tttgttttgg ccgaccttgg aaagcgcgcg acgcagcggt
       61 ttcgcgccgg ggagcgggaa ggtcatgttc cagaaattga tgtcggtgga gaggaattcg
      121 aggccaacgt aggtcttgcc gtcgcgcttg cgctgaatgg aagcaacgtg ggcctcgact
      181 ttgcgcgcgc tgttttcgtt gatcagcaga agaacctgcc cgacgaccag gggatgctcg
      241 gtctcgatct gcgcgccgtg ctggctcacc gcgttggtct tggcgcgaat cgagaatttc
      301 tccgaatcct cgttctgacc attcaatacc aaaggcacgg tcagagaaac gcgctgagtg
      361 cgccggcgct ctttgcagcg aaagtgaagt agggacatac gtgcaatacc ccgccaggct
      421 tcgatcttag ggacgcttcc gtgaggagga cagagccttt cgaaagccgc ttgggccact
      481 gcggcgagtt cttgctccgg ctgctgacgg tactacgcat ccattgcagc catattcgca
      541 gttgcccgca gcgagaacaa tagcgaagcg gtaccagaac acccgaccta gcagatagta
      601 cggtagtacc ttgtcctagt caggtcaacg gcaaatttcg tcagtttact atattcccct
      661 gcgattccta ccggaaagct cgggcaacac acctttccag gtaagcatcc atttcgcagg
      721 agcagtcgcc cccgcaccat cccatgcccc cgacgcttct cgctcgaact catccgcgtt
      781 cagggagacc tcgaaatgaa gccctttgcc ttgctgttgc tgctgtgcag tattgcctgc
      841 gcgccacgct cgtccgcaca agaccacttc gccgtgggag tctatgccga ttacttccgc
      901 ctgtctcaaa ccgacactaa ctcggcgggc ctagggggcc gtctcggatt cggaatcgcg
      961 ccgcacgtca tgctggaagc cgaggtcaat tacgatttcg gcgttacctt cacggagact
     1021 ttcgcgaacg gcggcagcct ggtgtttcag cgaactgaca tgaggctgct gcacgggctt
     1081 ttcggaccca aagtctcgtt gggtcattcc aattttcact tttttgtaac cgccaagggc
     1141 ggatttctcg acaccatgct ggattcgcgc ccggcgacgc tgggaacttt cctgagcagc
     1201 gtagacaatc tgcgttcgca gaacatcagc gccgtattct atccaggagg aggcgtggaa
     1261 gggcgcatcg gcccaatcgg attgcggctg gatgcgggcg acgaaatgta cttcaatagc
     1321 ggcacgcacc acaacctgcg cctggctttc gggccttatg ttcgcttttg aaggaatcgg
     1381 ctgaccctgc aaggattttt gtgcgacaaa tgtgccgcta gcgatcagga cggcggggtg
     1441 cttgctttcc ccgggcgcgc cgacaacttg taaacgccgg cgaatccttc ttaccgcaaa
     1501 gacggtgaaa aagggtcctc aagtctttac tgcatgcgag tcaaaccgag caagaagaac
     1561 aatttgcgga ttggccgagt ggcgcggaat ttgctcttct aagacacgcc ccagccgaac
     1621 ttacgcttga ggcggatgat gaaatccttt ttcgcgaacc cactggcctg cgcctggctg
     1681 gccggagcac ttactctaac cgtgccctcc ctggtggcgg cacaggaacc atctcaatcg
     1741 ccacaggacc agggacaatc gcaacagcgc gacaacggtc aagcaacggt tgcagaaaac
     1801 tcgttcagcg ggcgcatcac caaagcaaac aacgggaagt tcaggttgga ggatcgttcg
     1861 acgcgaactt cgtttgcact ggataaccag aagctggcga agaagtacga aggcaagaat
     1921 gttgtagtga ccggcacgct ggatgaggca aataacatcc tgcacgtaag aaagatcgaa
     1981 ctggccgtgt aagaaaagcg cccgcgaaag gtgcgccgag cgaggaggag tctgcctggc
     2041 tcctccttcg cctggactct ttggaactcc aggcgcaatg ctacttgcat gacgcggttg
     2101 tcggtcagcg tggacctagc gattccgaac gatgtttgat gctccagctt cacattcacg
     2161 gatcgaaacg gacactattg ctcacggccg gaatgcgctg cgcactattt tttccgcccg
     2221 gtcagcccga tttgtaaccg gttcgctatt tgcagtttga ctacgactgt actccgcatt
     2281 ccggccctcg ccggacccct gtgctacatt gcgaaattgg cgctacgtgt gcgcctgcat
     2341 gtgaacagcc agctgcaaaa cgatccgcga cgcgccctta acccggagaa agtaaaacgg
     2401 gtagaggtat attggcacgc cgtcacctat aaaacgctga tcgcctacgg gctgattctc
     2461 gcgctagtca ttttctcggc tgtgtatttt gtcacgcccg gcttttatgc cgctgtgatc
     2521 aggaaagtca gcagcgcggt tggggggacg gacacggaag tgactccgct cgccaacacg
     2581 caggctaaat tcgtaaatct tgatgggcgc gtgcaggtta aaaaagtgag ctcggtgcag
     2641 tgggtggacg ccgactaccg caccaccctg gacaaaggcg atttgatcca gacgggttcc
     2701 gatggcgctg cgcgcatcac tttcgccgac accacctcgt acaccatcaa ggccgacacc
     2761 ctggttaccg tcgaagaaaa caatgtgacg cgcgaccagg ccacgaccgc ggttcgcatc
     2821 aacaccggtg cggtggatct ctccaccgcc agttcgccga attcgcacac tgccgtcagc
     2881 gtggaagaca cggttgccca gatccgcccc aacagccgtg caactgtgaa atcggatccg
     2941 caatcgaagg aagccgaatt tacgatcgcc agcggcagcg cggaagtcca acgcggcggg
     3001 gaaaaagtgg aactgggtca gtggcagcgc gcgaacgttc gttccggcag tcccatctcc
     3061 acctcggatg ttttggctcc gcctgacctc gctcaaccgc taaacctggc tcccatcatt
     3121 actcaggtgc ccaagaccac gccggttcat tttgagtggc atctcgtcaa ggatgcggtt
     3181 acgtacaatc tgcggatcag caccaccgcg atgttcacca aacttgtaaa agatctgaaa
     3241 ctggctggca cttccgcgga cattaccggt ctcgacgccg gcgactattt ctggaacgtc
     3301 atcgccgtcg acggcaaaaa gcagaccagt gagatcagcg aaacgtttaa attcacgatc
     3361 gtcgtccagg gcaagtccca ggagatggtt ctggaaatcg gcggtacgca gttgcacggg
     3421 cgcgttgctg aagtctttgg ccggacggaa cccggtgcgg cgctcatcat aaacggccag
     3481 ccggttccca acatcggatc agacggcagc ttccggtact tcacggagcc gctcgatccg
     3541 ggcgagcaca ccatcgtgat tgttggccag aaccgccgcg gtggcactgc caaacagcag
     3601 gtgaccatcg tcgtcccgaa atgagttggc acaggtgccg ggcggcgttt ttgcgtcc
//
LOCUS       DASTPM010000599         6474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1145752, whole genome shotgun sequence.
ACCESSION   DASTPM010000599 DASTPM010000000
VERSION     DASTPM010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145752"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VFR42_10540"
     CDS             <1..1221
                     /locus_tag="VFR42_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369640.1"
                     /translation="DLLEKVAVACGLEDRMHPSILSTLFHMRKMTPEEKRAAAVDKLR
                     RDLRIEPIKKSFLISVSYASVDPQLSANVLNTLGNDYLEKHAAVRRPAGTQKFFENEV
                     DRYRQKLTDAQNSLADFNRKEGLVTNETEKDAAVPKLAEFEFNMRQTQASVPQAEEHV
                     RNLERQLASTPPRITTELRHSDNAVLMQQLKSSLVNLELQHTDLMHKYAPTDRQVKEV
                     EQQIEQVKSTIAAQEKAPVKEEATNENPTFALVREELVKARAELASLRALSTSYTKVG
                     AAYQSSAIERDQKQLEQQELLRNVKTAEQNYLLYLNKKEEARISDAFDQKRIVNVSIA
                     QAASVPAFPSNPASMLLSIAAVLGCLMGLGAAFVHEQIDSSMGSPDQVEAYLNMPVLA
                     AMPDEIPLRRLPNV"
     gene            1298..2092
                     /locus_tag="VFR42_10545"
     CDS             1298..2092
                     /locus_tag="VFR42_10545"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEU0369641.1"
                     /translation="MSRNFEILQRAGKVRGLFAADPETLPKFTARPMPENSPEPGPSI
                     PPGRRLQLGLEGAGREEAIRLVHRVFLSPGVDGPRAVVFSGVERRVGCTWNCAWAARA
                     LAAHVSGRVCVVDANLRHPGLHREFGLGNSRGLVGALSEKGPVISYASQLGDTNLWIL
                     PAGTDGTDLSRVCTPMEMDLRVNELESQFEFVLIDSPALNLYADALALGQMSDGIVLV
                     LESSSTQRLSARRAKQNADAHRVRVFGAVLNRFSQNVPDLVNRILK"
     gene            2089..4080
                     /locus_tag="VFR42_10550"
     CDS             2089..4080
                     /locus_tag="VFR42_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEU0369642.1"
                     /translation="MNSPISSFDADQDVAQADAVAERLTTSDRFFARLRKNQARMAIT
                     GADLVLAMISYWVAVNLFRDIRGEVWTREVLSGTLGILLACRLAGLLSAGLPGTSLRY
                     PSSSDVLRVIRGVSIGSVLFLLVSRIAHFRHEIPAALFVLDAAILVSLWGLLHCGVRL
                     LGSLRAAFRTKGKRVLIVGAGDAGMSLAKELTSDPGAPCIPVALVDDDKTRTGRNVYG
                     VPIAGAIDDLAAVASQSHAEEVLICIPSATRPEMSRILSACRQADLPVRTLPTLTELI
                     DGRVSQNELRTPNISDLLNREEMHQDAGEIAKIVRGKTVLVTGAGGSIGSELSRQIAS
                     GSPSKLLLLDKSENSLFYIHRALRERYPDLLLRPLLIDLTCRDFVDEFMHEEKPEAVF
                     HAAAHKHVGLIELHPHEGIRNNVLGIRNVALAALRCGAALFVNISSDKAVNPRNFMGC
                     SKRITEMFIQDLSRSNVTRFMNVRFGNVAGSTGSVLRLFWEQIQKGGPLLVTDPRASR
                     YFMTIPEAVFLILQAARLGHGGETFVFEMGEPINIYELAKNISLLAGMKPGKEVPIHF
                     VGLQKGEKVAEELWEEWENPVPTEQAGIQKVSEPDPEAVGMLEKIQRIERLITRNDRE
                     SLRQYLSILVPQFEKNRQEMFLEDEPLPIFVETQPGVQP"
     gene            4077..5342
                     /locus_tag="VFR42_10555"
     CDS             4077..5342
                     /locus_tag="VFR42_10555"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU0369643.1"
                     /translation="MSSTALKIPLSAPDVGDRELRYVEQVMRSGSLSLGPMLPRFEEA
                     FAATIGRKFAIAVNSGTSALHLCVRAAGIGAAHEVITTSFSFVASTNCLLYENAVPVF
                     VDIDAATLNISPAQIEEFLKNCCARNAQGRLINRATGREVKAILPVHVFGLPADMQAI
                     GDLAKEYGLAIIEDSCEAIGATYRGQQAGTFGDMSCFAFYPNKQMTTGEGGMIVTDDQ
                     PFARLCRSMRNQGRGEDGAWLKHERLGYNYRLSDIHCAIGLAQIERLAEMLEKRERVA
                     RRYHRALQGIPHLRRLADAADARRSWFVYIVQFAPYVDRGMRDRILQMLRAAGVGCQA
                     YFPAIHRQPFLKDLIHLPFGRLHDTETAADRCLALPFSSSMREEQIQYVAEALRNAVA
                     EECDVFAALAGGDHNSRKQFADESLQERV"
     gene            5339..>6474
                     /locus_tag="VFR42_10560"
     CDS             5339..>6474
                     /locus_tag="VFR42_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0369644.1"
                     /translation="MNSSAAQMSLQDVQSAVNRMPQVDAAVDTRQVTGIHLGHNAAAA
                     LVEDGKLLFAMQEERLSRVKNQGGLPVLTLDYIKGLANAQQGANVSREVALGGKNLSL
                     CYWRRDDILRTAGSAAPNIVGRAKNLARSNAAISDWINQRKWSGIAENLEPVMGGADV
                     PVHGIDHHLCHAASAYFGWGKMDEDVLVLTCDGAGDRLCATINIARNGKIRRVAGVDE
                     SHSIGALYGKITYLSGMVPMEHEYKLMGLAPYAEKARESQSISEDFEALFAFDRDNPM
                     LWTRSADCPPLQYSAEFLSNLLRRKRFDHIAGGVQLFVERFLVRWVQNCIAETGVRKV
                     ALSGGVFMNVKANQKILNLPEVEELFVFPSCGDETNSIGAAWLL"
BASE COUNT         1387 a         1715 c         2025 g         1347 t
ORIGIN      
        1 gaccttctgg aaaaagtggc cgtggcatgt ggcctggaag atcgcatgca cccgtcgatt
       61 ttgagcacgc tcttccatat gcgaaaaatg acgccggaag agaagcgtgc agcagcggtg
      121 gataagctcc gacgcgattt gcgcatcgag ccgatcaaga aatcgttttt gatctcggtt
      181 tcatacgctt ccgtcgaccc gcagctttcc gccaacgtgc tgaacacgct gggcaacgac
      241 tacctggaaa agcatgcggc ggtacggcgg ccggcgggca cccagaagtt ctttgaaaac
      301 gaagtggatc gctaccggca aaagttgacg gacgcacaaa actccctggc ggatttcaat
      361 cgcaaggaag gtttggtcac gaacgaaacg gagaaagacg ctgccgtgcc gaagctggcg
      421 gaattcgaat tcaacatgag gcagacacag gcgtcggttc cgcaggccga agagcatgtg
      481 cgcaacttgg agcgccagtt ggccagcacg ccgccgcgaa ttaccacgga attgcggcac
      541 agcgacaacg cagtgctgat gcaacagctg aagtcttcgc tggtcaatct ggagttacag
      601 cacaccgacc tgatgcataa gtacgcgcca accgatcgcc aggtgaagga agtggagcag
      661 caaattgagc aagtgaagtc cacgattgcc gcgcaagaaa aggctcccgt gaaggaagaa
      721 gcgaccaacg agaacccgac gttcgcgctg gttcgagaag aattagtgaa agcgcgcgcg
      781 gaactggcgt cgctcagggc tctgtccact tcgtacacga aagtgggcgc tgcctatcag
      841 agctccgcca tcgagcgcga ccagaaacag ttagagcagc aggaactgct gcgcaatgtg
      901 aagacggctg agcaaaacta cctcctctac ttgaataaga aggaagaagc gcggatttcc
      961 gacgcgttcg accagaagag gattgtgaac gtgtccatcg cgcaggcggc cagcgtgccg
     1021 gcgtttccat cgaaccccgc gtccatgctg ctgtcgatcg ctgcagtgct gggctgcttg
     1081 atgggcctag gcgcggcgtt tgttcacgaa caaattgact cttccatggg ctcgcccgat
     1141 caagtcgaag cgtacttgaa catgccagtc cttgcggcaa tgcccgacga aatcccattg
     1201 cggcgattgc ccaatgtgta gtggcgcacg ttgaagccag ccgggcgatt tgtgtgtcaa
     1261 cgacgcagct ctcaaaaact gggggaacaa gccaactgtg agtagaaact tcgaaattct
     1321 ccaacgggcc ggcaaggttc gaggattgtt tgccgccgat ccagaaacat taccaaagtt
     1381 tacggcgcgg cccatgcccg agaattcgcc ggaaccggga ccatccattc ccccgggccg
     1441 tcgattacag ctgggtctgg aaggcgcagg gcgtgaggaa gcgatacgcc tggtccaccg
     1501 ggtgttcttg tcgcctggcg ttgatggtcc acgcgctgtg gttttttcag gagtcgagcg
     1561 gagagtcggt tgtacatgga actgcgcctg ggcggctcga gcgctggccg cgcatgtttc
     1621 cggccgtgtg tgcgtggtgg atgcgaatct gcgtcatcct ggcttgcacc gggaatttgg
     1681 gctgggcaat tcgcgtggac tggtcggtgc actcagcgaa aagggccctg tcatcagcta
     1741 cgccagccag ttgggtgaca ccaacttatg gattttgccg gcaggaacag acggaacgga
     1801 tctttcacgc gtctgcacgc cgatggaaat ggatttgcgt gtcaacgaac tcgagtcgca
     1861 atttgaattt gtgttgattg actctcctgc tttgaacctg tacgccgatg cattggcgct
     1921 tggccagatg tccgacggga tcgttctggt gctggaatcc tcttcgacac agcggctctc
     1981 cgcccgacga gccaagcaaa acgccgatgc gcatcgagtg cgagtttttg gtgctgtgct
     2041 gaatcgattt tcgcaaaacg ttcccgacct ggttaaccgg atactcaaat gaattcaccg
     2101 atctcaagtt ttgacgcaga tcaagacgtg gcgcaggctg atgcggtcgc ggaacgcttg
     2161 acgacttcgg accgcttctt cgcgcgcttg cgaaagaatc aggcgcgcat ggcaattacg
     2221 ggagcggacc tggtcctggc catgatttcg tactgggtcg cggtaaatct gttccgagat
     2281 attcgcggcg aggtttggac gagggaagtg ctgtccggca ctctcggaat cctgctggcg
     2341 tgcaggctgg cgggtcttct ttccgcgggc ctgcccggaa cttcactacg atatccgagt
     2401 tcatcggatg tgctgcgggt gattcgcggc gtgtcgatcg gaagcgttct atttctgctc
     2461 gtatcgcgca ttgcccattt ccggcatgaa attccggcgg cactttttgt gctggacgcg
     2521 gcaattctgg tttctctgtg ggggttgctg cactgcggcg tgcgcttact cggttcgctg
     2581 cgtgccgcgt tccgcaccaa aggcaagcgt gtgttgattg tcggcgcggg cgacgccggg
     2641 atgagccttg cgaaggaatt gacttccgat cccggtgctc cgtgcatacc ggttgcgctg
     2701 gtggacgacg ataaaacacg caccggccgc aacgtgtacg gcgtaccgat cgcaggcgcc
     2761 attgacgatc tggcggcggt agccagccag agccatgcgg aagaggtgct gatctgtatt
     2821 cccagcgcca cacggccgga aatgagccgg atcctgtccg cttgtcggca agccgacctg
     2881 ccggtgcgta cgcttccgac gctcaccgaa ttgattgacg gccgagtttc gcagaatgaa
     2941 ttacgcacgc cgaacatcag cgatttgctc aaccgcgagg aaatgcatca agacgcgggc
     3001 gaaattgcca agatcgtgcg cgggaaaacg gtgttggtca cgggtgcggg cggtagcatt
     3061 ggctccgagt tgtcgcgtca gatcgcttca ggctcgccgt ccaagctgct gctgctcgat
     3121 aaatcggaga acagtctttt ctacatccac cgcgcgctgc gtgaacgcta tccggatctt
     3181 ctgctgcggc cgttgttgat cgacttgacc tgccgagact ttgtcgacga attcatgcac
     3241 gaagagaagc cggaggcagt gttccacgcc gccgcgcaca agcatgttgg gttaatcgaa
     3301 ctgcatccac atgaaggcat ccgcaacaat gtgctgggta ttcgcaacgt ggcgctggcg
     3361 gcgttgcgct gcggagcggc gctgtttgta aacatttcct cggacaaggc agtgaacccg
     3421 cggaatttca tgggctgttc gaagcgcatc accgaaatgt ttattcagga tctgtcgcgt
     3481 agcaacgtga cgcgcttcat gaacgtacgc tttggcaacg ttgcgggaag cacgggaagc
     3541 gtgctgcggc tgttttggga gcaaatccaa aagggtggcc cgctgctggt cacggacccg
     3601 cgcgcttcgc ggtatttcat gaccattccc gaagcggtgt tcctgatttt gcaagcggcc
     3661 aggctgggac acggcggcga aacttttgtg tttgaaatgg gagagcccat caacatttac
     3721 gaactggcga agaacatcag cctgttggcg ggaatgaagc ccggcaaaga agtgcccatt
     3781 cattttgtgg gtcttcaaaa aggagaaaaa gtggcggagg aactttggga ggaatgggaa
     3841 aacccggtgc cgaccgagca ggcgggaatc cagaaggtaa gcgagccgga tccggaagcg
     3901 gtaggcatgc tggaaaaaat ccagcgcatc gaacgcctga ttactcgcaa cgaccgcgaa
     3961 agtctgcggc agtacctgtc gattctggtg ccgcaattcg agaagaatcg ccaggaaatg
     4021 tttttggaag acgagccgct gccgattttc gtggaaacgc agccgggagt gcagccatga
     4081 gctcgactgc cttgaaaatt ccgctgtccg cgccggatgt aggcgatcgc gaacttcggt
     4141 atgtggagca ggtgatgcgt tccggcagtc tgagcctggg cccgatgctg ccgcgtttcg
     4201 aggaggcatt tgcggcgacc atcgggcgaa aattcgcgat tgccgtcaac agcggcacgt
     4261 cggcattgca tctgtgcgta cgagcagcag gcatcggcgc cgcgcatgaa gtgattacga
     4321 cttcctttag ctttgtggcc tcgacaaact gcctgctcta cgaaaacgcg gtaccggttt
     4381 ttgtggacat tgatgcggcc acgctgaaca tttctccggc gcagattgag gaatttctaa
     4441 agaattgttg tgcgcgcaac gcgcaagggc ggctgattaa tcgagcgacg ggcagggaag
     4501 tgaaagccat cctgccggtg cacgtctttg gcttgccggc cgatatgcag gcgattggcg
     4561 acctcgcgaa agaatacggc ttggccatca ttgaagactc ctgcgaagcg attggcgcaa
     4621 catatcgtgg ccagcaagcc ggcaccttcg gcgatatgtc ttgctttgcc ttttatccga
     4681 acaagcagat gaccaccggt gaaggcggca tgatcgtcac tgacgaccaa cctttcgcgc
     4741 gactgtgccg cagcatgcgc aaccagggcc gcggcgaaga cggcgcctgg ctgaagcatg
     4801 aaaggctcgg ctacaactat cgcttgagcg atattcattg cgcgattggt ctggcacaga
     4861 ttgagcgctt ggccgaaatg ttggaaaaac gcgagcgggt ggcgcgacgt taccaccgcg
     4921 cgctgcaagg cattccgcat ttgcgcagac tggccgacgc ggcggatgcc aggcgcagct
     4981 ggttcgtcta tatcgtgcag tttgcgccat atgtggatcg cggaatgcgc gatcgaattc
     5041 tgcagatgct gcgcgcggcg ggcgtcgggt gccaggcgta cttcccggcc attcaccggc
     5101 agccctttct gaaggacctg attcatttgc cgttcggaag gctgcacgat accgagactg
     5161 cggctgaccg ctgcctggcg ctgccgtttt cctctagcat gcgtgaagaa cagattcaat
     5221 acgttgcgga ggcattgcgc aacgcagtgg cggaagagtg cgacgttttc gccgcgctgg
     5281 caggtggcga ccacaattcg cgtaagcagt tcgcagacga atctctacag gagcgagtat
     5341 gaacagttca gctgcacaaa tgtcattgca ggatgttcag tctgcggtga accggatgcc
     5401 gcaggtggac gccgccgtag acacccgaca ggtcacgggt atccacttgg ggcacaacgc
     5461 cgcggctgcg ctggttgagg acgggaaact gctgtttgca atgcaggaag agcggctgtc
     5521 tcgcgtgaaa aatcaaggcg ggctgcctgt attgacactc gactacatca aagggctcgc
     5581 gaacgcgcag caaggggcga acgtttcacg cgaggttgcg ttgggcggca agaatttgtc
     5641 cctttgttac tggcggcgcg acgacatctt gcgcacggcg ggcagcgcgg cgcccaacat
     5701 tgtggggcgg gcgaaaaatc tggcgcgcag caacgcggcc atctccgatt ggatcaacca
     5761 gcggaaatgg agcggaatcg ccgagaacct ggaacccgtg atgggtggcg cggacgtgcc
     5821 agtacacggg atcgaccatc acctgtgtca cgcggcttcg gcctatttcg gatggggaaa
     5881 aatggatgag gacgtgctcg tcctgacgtg cgatggcgcc ggggatcgct tgtgcgcgac
     5941 gatcaacatt gcgagaaacg gaaagatccg gcgcgtagcc ggcgttgatg aaagccattc
     6001 catcggcgcg ctgtatggaa agatcaccta cctttcgggc atggtgccga tggagcacga
     6061 atacaagttg atggggcttg ctccgtatgc cgaaaaagcg cgtgagtcgc agtcgatcag
     6121 cgaagatttt gaagcgctgt ttgcttttga ccgcgacaac cccatgctgt ggacgcgatc
     6181 cgcggattgt ccgccgttgc aatacagcgc tgaattcctt tcgaacctgc tgcggcgtaa
     6241 acgcttcgat cacattgcgg gcggggtgca actcttcgtg gagcgtttct tggttcgctg
     6301 ggtgcaaaac tgtattgcgg aaacgggcgt tcgaaaagtg gctttgagcg gcggcgtctt
     6361 catgaacgtc aaagccaatc agaaaattct gaacctgccg gaagtggagg agttgtttgt
     6421 ttttccgtct tgtggagatg aaactaactc gattggtgcg gcctggttgc tgga
//
LOCUS       DASTPM010000600         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_742470, whole genome shotgun sequence.
ACCESSION   DASTPM010000600 DASTPM010000000
VERSION     DASTPM010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742470"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFR42_10565"
     CDS             complement(<1..720)
                     /locus_tag="VFR42_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0369645.1"
                     /translation="MKFCRFIAATSATAEPRYGILEKASVREISSSPWTEWRETGKSM
                     PVAEVRLVAPVEPRKIVCIGRNYAAHAAELGNEVPKDPLIFLKPSTAIIGPGESVVLP
                     KYSRRVEHEGELGLAIGRRCSHLSDTEDALSYVLGYTCVNDVTARDLQKTDVQFTRAK
                     GFDTFCPVGPHIETQFRPEDVLVETLVNGELRQSGRTSLMVFPPAFLVRWVSRMMTLE
                     PGDLIATGTPAGVGPLLAGDSV"
     gene            complement(755..1915)
                     /locus_tag="VFR42_10570"
     CDS             complement(755..1915)
                     /locus_tag="VFR42_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369646.1"
                     /translation="MVNRKGPRLCDQSFAALRGLFCLALFGATAGCGAPGEPQPPSPP
                     IPAAITDLAAHQQGNGVRLVFTLPGRTTAGERLAEPPATEIFRGTLKPDGKPDNKSFR
                     LVYTIPGALADTYAAQGKIQFTDPIAAEELRTHPEMQLAYRLRTRASKKRDSADSNTV
                     AVRLYGVADKIRAVSAHVTQTAVELSWPSPKAGASSSDTVAAYHIYRGELHPDAPVPT
                     NGDLSQAKWKNPPLLLAPAQSNSYSDTFFEFGKTYVYLVRSVTIAGGSPIESDDSAPA
                     IVTPRDTFPPATPQNVVAAVLPGDHDRRIVDLSWSINVEADLAGYRVYRSEKPGERGQ
                     LLQTELLPSPAYRDSAVQAGHRYWYTATAVDRAGNESGSSDAALADLTQPAP"
     gene            complement(1933..>2518)
                     /locus_tag="VFR42_10575"
     CDS             complement(1933..>2518)
                     /locus_tag="VFR42_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0369647.1"
                     /translation="MGIIFIDEIDKVAGRESGHGPDVSREGVQRDILPIIEGTTVNTR
                     YGMIRTDHILFVAAGAFHVTKPSDLIPELQGRLPIRVELQSLKEEDFVRILTEPKNAL
                     IKQYIALLETEGLKLNFTDDAVETIAKFATQVNEQTENIGARRLHTILEKVLDELSFE
                     APDLKKKNVKVDAAYVNKQLADIVKNQDLSRYIL"
BASE COUNT          497 a          764 c          719 g          538 t
ORIGIN      
        1 gacggaatct cccgcaagca agggtccgac tccggcggga gttccagtgg cgatcaagtc
       61 gccgggttcc agcgtcatca ttcgcgaaac ccagcgcacc agaaaagccg gcgggaacac
      121 catcaatgaa gttcgccccg actggcgcaa ttcgccgttg accagcgttt ccaccagcac
      181 gtcctccggc cgaaactgtg tttcgatgtg cggacccacc gggcaaaacg tgtcgaaccc
      241 cttggcacgc gtgaattgca catcagtctt ctgcaaatca cgcgccgtca catcgtttac
      301 gcaggtgtag cccagcacgt aggacaacgc atcctcggtg tcgcttaaat gcgagcagcg
      361 gcgcccaatc gccagcccca gctcgccttc atgctccacg cgccgcgaat attttggcaa
      421 gacgaccgat tcgcctggac cgatgatggc agtggacggc ttcaagaaaa tgagcggatc
      481 cttgggcact tcgttgccga gttccgccgc gtgcgcggcg tagttgcgcc cgatgcacac
      541 gatttttctc ggctcgaccg gcgccaccag ccgcacttcg gccaccggca tcgacttccc
      601 cgtttcccgc cactccgtcc atggagacga gctgatttcc cgcacgcttg ctttttcgag
      661 aattccatag cgcggttcgg ctgtcgcgga tgtggcagcg ataaatcgac aaaacttcat
      721 gggaaaagag tgtatcagga ctgaccggct ggcttcaggg agcaggctgc gtcaagtcgg
      781 caagcgctgc atcagatgac ccgctttcat ttcctgcgcg atccaccgcg gtggcggtat
      841 accagtaacg gtgtccggcc tgcacggccg aatctcggta agccggagaa ggcagcaatt
      901 cggtttgcag caactgcccg cgctctcccg gtttctcaga acgataaacg cgataacccg
      961 caaggtccgc ctcgacgttg atggaccagg acaaatctac gatgcgacgg tcgtggtcgc
     1021 ccggcaagac ggccgccacc acattttgcg gcgtcgccgg cggaaacgta tcgcgtggag
     1081 ttacgatggc cggagcggag tcgtccgatt caatcgggct gccgccggca atggtcacac
     1141 tgcggaccaa gtaaacgtaa gtcttcccaa actcaaaaaa cgtgtcgctg taagaattgc
     1201 tctgcgcagg agccaacagg agtggcgggt ttttccattt ggcttgcgaa aggtcgccgt
     1261 ttgtgggaac cggtgcgtca ggatgcaatt cgccgcgata aatatggtac gcggcgacgg
     1321 tgtcgctcga actcgcgcca gctttcgggg aaggccacga cagctcgact gcagtttgcg
     1381 ttacatgcgc gctcaccgcg cgaattttgt cggctacacc gtacagcctt acggccacgg
     1441 tgttcgagtc ggcggaatct cttttcttag aagcgcgggt tcgcaggcga tacgccaact
     1501 gcatttccgg atgtgtgcgc aactcctctg cggcgatcgg atcggtaaat tgaatttttc
     1561 cctgcgccgc gtaggtgtcg gccagcgctc cgggaattgt gtacaccagg cggaacgatt
     1621 tgttgtccgg ctttccgtca ggcttcagtg tgccgcgaaa gatttccgtt gctggcggtt
     1681 cggccaggcg ttcgccggcg gtcgtgcgcc ccggcaaggt gaacaccagc cgcacgccgt
     1741 tgccctgctg atgcgcagcc aagtcggtaa tcgccgccgg aatcggtggc gaaggaggct
     1801 gcggttcgcc gggagcgcca caaccagcag tcgcgccaaa cagcgccaaa cagaaaaggc
     1861 cccggagcgc ggcaaaggat tggtcacaaa ggcgcggccc ttttctgttt accatttttc
     1921 agttttcggt cattacagaa tgtagcggct caaatcctgg ttcttcacaa tatccgcaag
     1981 ctgcttgttc acgtaagcgg catctacctt cacgttcttc ttcttcaggt ccggcgcttc
     2041 gaatgagagt tcgtccagca ctttttccag aatcgtgtgc aaccgtcgcg cgccgatgtt
     2101 ttccgtttgc tcgttcacct gggtggcgaa ctttgcgatg gtctccaccg cgtcatccgt
     2161 gaaattcagc ttcagtcctt ctgtttccag caaggcgatg tactgcttga tgagcgcatt
     2221 cttcggctcg gtgagaatgc gcacgaaatc ttcttccttc agcgattgca gctccacgcg
     2281 aatcggcagg cggccttgca actccgggat caggtcggaa ggcttggtca cgtgaaacgc
     2341 gcccgccgcc acaaacaaaa tatggtcggt gcgaatcatg ccgtagcgcg tgttcaccgt
     2401 cgtgccttcg atgatcggca aaatgtcgcg ctgcacgcct tcgcggctca catccggccc
     2461 gtgtccggac tcgcggcccg ccaccttgtc gatttcatcg atgaaaataa ttcccatc
//
LOCUS       DASTPM010000601         6020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_165588, whole genome shotgun sequence.
ACCESSION   DASTPM010000601 DASTPM010000000
VERSION     DASTPM010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165588"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VFR42_10580"
     CDS             complement(<1..883)
                     /locus_tag="VFR42_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0369648.1"
                     /translation="MVTKKAQLLLVDDDANTLASLSRAFRLAGHEATVCDNANRAVEL
                     LRSEKFDLIFSDVVMPGKSGLEFLEEIKNAGVKAPVILISGQANIEMAVRATKLGALD
                     FLEKPLSTDKLLVTVENALRLTRLEDENRELKRRLGKHQLVGSGPAMKKLIAQIDRVA
                     SSETRVCILGETGTGKELVARAIHEKSPRHDGPFVTLNCAAVPAELIESELFGHEKGA
                     FTGAAGRHIGKFEQAEGGTLFLDEIGDMPVTMQAKLLRVLEEGEVERVGGDKPVKVNV
                     RVVVATHRNLEELVAQNA"
     gene            complement(898..2766)
                     /locus_tag="VFR42_10585"
     CDS             complement(898..2766)
                     /locus_tag="VFR42_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0369649.1"
                     /translation="MSFRTKLFLLFLVTVLASVSAVTYSVTHYTRAAYEEMDAQRTTA
                     LVAQFQKELAQQREIVAQHVENVTNADVTLKMAIDQTKPDPDQSLYVRDASGAAQEHS
                     LDFMEFVIADGTLISSAEYPARVGYKETWVTSVKDWNGSEAFLRRVELPNEVAVSLTA
                     VRTQPNVAKPFYTVGGRRLDENFLASLVAPAGLRALLYLNLDQSFTPADVMAPTGKVD
                     QAEKFAPMIAQLQQQPGPLVETIQWTNDAESAETFHALPLTGRNGELLGALLVGSSRA
                     DLVHLTRHIVKLAAAVAAAAMLIGLLLTWWVSARITKPIEELAEGAREVAAGHWHTRI
                     DLRGQDETGQLAAAFNHMTQTLSAQRERLVQTERVAAWRELARRLAHELRNPLFPLQI
                     TVENMQRAQQLSHQQFLEVFNESTATLKAELANLNSIVNRFSDFSKMPPPEFSRLEIN
                     EVIRGAVRLFEPQFHEVGKPTITTEYFLKEGLPEIDGDPDLLHKAFQNLVLNALDAMP
                     AGGTLTFRTAETQTGVRIEVSDTGKGLTPEECSRLFTPYYTTKQLGTGLGLAIVQSVI
                     SDHRGTISVASEEGHGTTFRIELPKRQPGVTPKKAQPAVAEEPPPQTTAETAAAND"
     gene            complement(2763..4319)
                     /locus_tag="VFR42_10590"
     CDS             complement(2763..4319)
                     /locus_tag="VFR42_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0369650.1"
                     /translation="MRCIALRWCAAIKGLRRVVPVLCFAALPGIGAVRPQYGGVLRVE
                     LRAASANLNPAKWKAGSSDFAANQRLAELVYDRLVTLDNYGRFQPQLATEWSHDASAK
                     KWQFTLRQGVKFADGTPLTPGDVAAALQPLLPRGMQVVATSGGIAIQSAAPAGDLLEQ
                     LSSGAYFVYKDNGGGLLKGTGPFVLESVSAGVRDGDGANATGAAAQTQRLRFRSNENC
                     WSGRPYLDAVEVMLGVPPLKALLDLQLGKTDLSELSAEMSLRAQQTNLKCWASAPLTL
                     YALRFSGDARGESEQRLREAIGLSVDRGAMARVLLQKQAEAAGSFLPQWLSGYAFLFD
                     TESNLEHAKELRKMMAASAPGAAQALRIGVDGNSELSRLIAERVVVDARAAGLTLQTV
                     KGNIRPIGDGSGSKSEGEAQLIAWRYSSLSPRTALQDVAGAWRQTAESAVPGDADARY
                     AWERKMMDQRNLLPLVAVPDFAAVDARVRNWAPAPWGEWRLADVWLEQGEGANALRGA
                     AKKAPSRGKP"
     gene            complement(4282..5232)
                     /locus_tag="VFR42_10595"
     CDS             complement(4282..5232)
                     /locus_tag="VFR42_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369651.1"
                     /translation="MTMAPAVARPDALEDGAERLAKKAAALPHERRMSLVWTNHAALT
                     EPRVERLRAAFTAQMEATQVRLVQGEMAPALRVAIELTPTQIVFTASVPAEGSTSVTI
                     EEVARAAAGIDASPGNGVRLEKELLWQQEGKILSAGLLPAGADGGKRLVLLTEENLLV
                     YGGGPGNWRLENTKLLPGPRQAQRSARGQLIVATESNEPVGVLLPGRRCEADVADDSS
                     VACANLTTEWPSGRLMARPSCGAQTWWLKSDGVDWTEEDRLLLRSAGAGREAAAAELG
                     FAGPVISVSGGDNPGSATAVARNLNSGNYEVYRVALVCGN"
     gene            complement(5458..5898)
                     /locus_tag="VFR42_10600"
     CDS             complement(5458..5898)
                     /locus_tag="VFR42_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369652.1"
                     /translation="MYSPGLVIVLAGAMCVAPVAAATKSGEETRVLSYRAVDRAEIEN
                     LQRWVAAGHEDWCKDARLVAAEELKRLAADFVDDATELTAVNIGESFDGSNGAKKLTF
                     EWTPLDGRAAYRVTVERFEWLLPIAGDAEAVVWVPTATEIQIHK"
BASE COUNT         1092 a         2205 c         1461 g         1262 t
ORIGIN      
        1 acgcattctg cgcaaccaac tcctccagat tgcgatgcgt cgcgacgacc acacggacat
       61 tcactttgac cggtttgtct ccgccgacgc gctcgacctc tccctcttcc agaacgcgca
      121 acagcttggc ctgcattgtc accggcatat caccaatttc atccaggaac aacgttccgc
      181 cttcggcttg ttcgaatttt ccgatatgtc ttcctgccgc gcccgtgaat gcgccttttt
      241 cgtggccgaa cagctccgat tcaatcagct ccgccggtac tgccgcacaa ttcaaagtga
      301 caaacggacc atcgtgccgc ggcgattttt catggatcgc ccgcgccacc aattccttgc
      361 cggtcccggt ttcgcccaga atgcaaacac gcgtctcgct gctggcgact cgatcgatct
      421 gcgcaatcaa ctttttcatg gccgggcccg agccgaccag ctgatgcttc ccgaggcgcc
      481 ttttcagttc gcgattctcg tcttccagcc gcgtcaaccg aagcgcgttt tcgaccgtaa
      541 ccagaagctt gtcggtggaa agcggctttt ccaagaaatc cagcgcgccc agcttcgtgg
      601 cgcgcaccgc catctcgata tttgcctggc cggaaatcag gatcaccggc gccttcactc
      661 cggcgttttt gatctcttcg aggaactcca gcccgctctt tcccggcatc acgacgtcgg
      721 agaagatcag atcaaacttt tcgctgcgca ataactccac ggcgcgattt gcgttgtcgc
      781 aaacggtggc ctcatgcccg gccagccgga atgcgcgcga caatgacgcc aatgtattcg
      841 cgtcgtcgtc caccagcagc agttgagctt tttttgtcac catcgtcttc ggttcgccta
      901 gtcattggcc gcggcagttt cggccgtggt ttgcggtgga ggttcttcgg caaccgccgg
      961 ctgagctttt ttgggcgtca ctccgggctg gcgtttcggt agttcgatgc gaaaagttgt
     1021 gccatgacct tcttcgctcg ccaccgatat ggttccccga tggtcgctga tcaccgattg
     1081 cacaatcgcc agccccagcc cggtgcccaa ctgcttcgtc gtgtagtacg gcgtaaagag
     1141 tcgcgagcac tcttccggtg tcagcccctt ccccgtgtcg gaaacctcga tgcgaacgcc
     1201 ggtctgcgtt tcggccgtcc ggaaagtcaa cgttccgcct gcaggcatgg catccaacgc
     1261 attcagcacc aggttctgaa acgccttgtg cagcaagtcc gggtcaccgt caatttccgg
     1321 caacccctct ttcagaaaat actccgtcgt gatggttggt ttgcccactt cgtggaactg
     1381 cggttcgaat agccgtaccg ctccgcgaat cacttcgttg atttccaacc gcgaaaactc
     1441 cggcgggggc atctttgaaa aatcgctgaa acggttcacg atcgaattca ggttggccag
     1501 ttccgctttc agcgttgcag tcgattcatt gaaaacttcc aggaactgtt ggtggctcag
     1561 ttgttgcgcg cgctgcatgt tttctaccgt gatctgcagc ggaaacagtg gattgcgcag
     1621 ctcatgcgcc agccttcgcg ccaattcgcg ccaagccgcg acacgctcgg tctgcaccaa
     1681 acgctcacgc tgcgcagaca gcgtctgcgt catgtgattg aaagccgcgg ccagctggcc
     1741 cgtttcgtct tgcccgcgca gatcgatccg cgtatgccag tggccggccg ccacttcgcg
     1801 cgcgccttcc gctagctctt caatcggctt ggtgatgcgc gcggaaaccc accaggtcag
     1861 caacaatcca atcagcatcg ccgcggccgc cacagctgcc gccagcttca cgatgtgccg
     1921 agtcaagtgc accaggtccg cgcgcgaact ccccaccagc agcgctccca acaattctcc
     1981 gttgcgtcct gtcagcggca gcgcgtgaaa cgtctccgcg gactccgcgt catttgtcca
     2041 ctgaatcgtt tccaccagcg gcccgggctg ctgctgcagc tgtgcgatca tcggcgcgaa
     2101 tttctccgcc tggtccactt ttcccgtcgg cgccatcaca tccgccggcg taaaactttg
     2161 atccagattc agatacagca gtgcccgcaa tccagccggt gccacgagcg acgccaaaaa
     2221 gttttcatcc agccgccgcc cacccaccgt gtagaaaggc ttggccacat tcggctgcgt
     2281 tcgcaccgcc gtcagcgaca ccgccacttc gttcggcaac tccacacggc gcagaaacgc
     2341 ttccgagcca ttccagtcct tgacgctggt tacccaggtt tccttgtaac cgacccgcgc
     2401 gggatactcc gccgaagaaa tcagcgtccc gtccgcaatt acaaactcca tgaagtccaa
     2461 actatgctct tgcgcggcgc ccgacgcatc gcgcacatag agtgactggt ccggatcagg
     2521 cttcgtctga tcgatcgcca ttttcagcgt tacatcggcg ttggtgacgt tctccacatg
     2581 ctgcgccaca atctcgcgtt gttgtgcaag ttccttctgg aattgcgcca ccaacgcggt
     2641 tgtccgctgc gcatccatct cttcgtatgc cgctcgcgtg tagtgggtca cgctgtacgt
     2701 caccgccgat acggaagcca gcaccgtcac cagaaacagc aagaacagtt ttgtgcggaa
     2761 gctcatggct tccctctcga gggcgctttt ttcgcggcgc cccgcaaggc attcgctcct
     2821 tctccctgct cgagccacac gtccgccagc cgccattctc cccatggcgc cggggcccag
     2881 ttgcgcacgc gcgcatccac cgccgcaaaa tccggcacag ccaccaacgg caaaagattc
     2941 ctctggtcca tcatttttct ttcccaagca tagcgcgcat ccgcgtcccc gggcacagcg
     3001 ctttccgcag tctgccgcca tgctcctgcc acatcctgca gcgccgtccg cggcgatagc
     3061 gagctgtagc gccacgcaat cagctgcgct tcaccctcac tcttcgatcc gcttccatcg
     3121 ccaatgggcc ggatgtttcc cttcactgtt tgcagcgtca aacccgcggc gcgcgcgtcc
     3181 accaccacgc gctcggcgat cagcctggac aactcgctgt ttccatccac gccgatccgc
     3241 agcgcctgcg ctgcccccgg tgcgctcgcc gccatcatct ttcgcaactc tttcgcgtgt
     3301 tccagattac tctccgtgtc gaagagaaaa gcatatcctg agagccactg cggcaaaaag
     3361 gaacctgccg cctccgcttg cttctgcagc aacacgcggg ccatcgctcc gcggtctacc
     3421 gaaaggccga ttgcctctcg cagccgctgc tcgctttctc ccctggcgtc tcccgaaaac
     3481 cgcagcgcgt acagcgtcag cggtgccgat gcccagcact tcaggttcgt ctgctgtgcc
     3541 cgcaaagaca tttccgccga aagctcgctc agatccgtct ttcccagttg caaatccagc
     3601 agcgccttca gcggcggcac gcccaacatc acctcgaccg catccagata cggccgtccg
     3661 ctccagcagt tctcgttcga acgaaatcgc aaccgctgtg tctgcgccgc agcgccggtg
     3721 gcattcgcgc cgtcgccgtc tcgcacgccc gccgaaacgc tctccagcac aaatggaccc
     3781 gtgcctttca gcaacccgcc gccgttgtcc ttgtaaacga agtaggcgcc cgacgacagc
     3841 tgttccagca agtctcctgc tggcgccgcg gactgaatcg caatcccccc gctcgtcgct
     3901 accacttgca ttccgcgcgg aaggagcggc tgcaaagccg ccgcaacatc gcccggcgtc
     3961 agcggcgtcc cgtctgcaaa cttcacgccc tgccgcagcg tgaactgcca cttcttcgca
     4021 cttgcgtcgt gactccactc cgtcgccagt tgcggctgaa atcttccgta gttgtccagc
     4081 gtcaccagcc tgtcgtacac cagctccgcc agccgctggt tggccgcaaa atcgctcgat
     4141 ccggccttcc attttgccgg attcaaattc gccgaagccg ctcgcagctc cacccgcagc
     4201 acgccgccat actgcggccg caccgcccca attcccggca gcgcggcaaa acacaacacc
     4261 ggcacgacac ggcgcaatcc cttaattgcc gcacaccagc gcaacgcgat acacctcata
     4321 gtttccgctg ttcaaatttc gcgcgactgc tgtggcgctt cctgggttgt cgcctccgct
     4381 gaccgaaatc accggacccg caaaccccaa ttccgcggcc gctgcttccc tgcccgcccc
     4441 cgcgctgcgc aacagcaggc gatcctcctc cgtccaatcc acgccgtcgc tcttcagcca
     4501 ccacgtctgc gctccacaac ttggccgcgc catcaatctc ccgcttggcc actccgtcgt
     4561 cagattcgca cacgccaccg acgaatcgtc cgccacatcc gcttcgcacc gccgccccgg
     4621 cagcaacact cccactggct cattcgattc cgtcgcaaca atcaattgtc cgcgcgccga
     4681 acgttgcgcc tgccgcggcc ccggcaacag tttcgtattt tccaatctcc agttccctgg
     4741 cccgccgcca taaaccagca aattctcttc tgttagcagc accaaccgtt tgccgccatc
     4801 cgcaccagcc ggaagcaaac cagcactcag aatttttccc tcttgctgcc acaacaattc
     4861 tttttcgagc cgcacgccat tcccaggact cgcatcgatt cccgccgccg ctcgcgccac
     4921 ctcctcgatc gttacgctcg tgctgccctc ggccggcacg ctcgccgtaa acacaatttg
     4981 cgtcggcgtc agctcaatcg ctacgcgcaa cgccggcgcc atttcccctt gcaccagccg
     5041 cacttgtgtc gcttccatct gcgcggtaaa tgccgcgcgc aaccgttcga cgcgtggctc
     5101 cgtcagagct gcgtggttgg tccacaccag cgacatgcgc cgctcgtgcg gcaacgccgc
     5161 agcttttttc gccaaccgct ccgcaccgtc ttccagcgca tcgggccgcg ccaccgccgg
     5221 cgccatcgtc atcagattga aaaggaaaaa gaatgctgcg caggcaacta ccaccagccg
     5281 aacgcgtggg accaatctct agtgcacggg agtgaagcga catcttagca cgtccctccg
     5341 ccaggacgcg gggcgcgccg cgcttcagcc ctgaacttgc cacggccgcc ccgccccatt
     5401 tccgcgcagc gaaccccttg ctctaggacc cgctgcgccg gaatccttcg cgccaaatca
     5461 tttgtgaatc tgaatttccg tcgccgtcgg cacccacacc accgcctccg cgtcgccggc
     5521 aatcggcagc agccattcga accgttccac ggtcacgcga taggccgctc gcccgtccag
     5581 cggggtccat tcgaaagtca gtttttttgc accattgctg ccatcgaagc tctcgccaat
     5641 gttcaccgcc gtcagctccg tcgcgtcgtc cacaaaatcc gcagcaagcc gcttcaattc
     5701 ttccgctgcc accagccgcg cgtctttgca ccagtcttca tgccccgccg caacccacct
     5761 ctgcaaattc tcaatctccg cccgatccac cgcgcggtaa ctcagcaccc gcgtctcctc
     5821 tcccgatttc gtcgccgcag ccaccggcgc cacgcacatc gctcccgcca gcacaattac
     5881 caaacctggc gaatacatga ctcctacgac cttaggaatt tctttcgttg catttctgcc
     5941 attcatgacc gcctccgctc tcgctctttc gtattttcga atttcggatt tcgaatttcg
     6001 attttccacc ttcacaccag
//
LOCUS       DASTPM010000602         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_137043, whole genome shotgun sequence.
ACCESSION   DASTPM010000602 DASTPM010000000
VERSION     DASTPM010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137043"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFR42_10605"
     CDS             <1..277
                     /locus_tag="VFR42_10605"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU0369653.1"
                     /translation="EAVRAPIEKLGGRIHNAFFAFGEFDVVVITEMPDNVSAAAIAIA
                     FAAGGSIKNEKTTPLMTTTEALEAIRKAGTSGYRSVTAPSSAAAART"
     gene            274..999
                     /locus_tag="VFR42_10610"
     CDS             274..999
                     /locus_tag="VFR42_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0369654.1"
                     /translation="MRLPSAYKSEQSGRSDRCAPFIEVANVTRFDWICMCVTGALLFL
                     FCAHPHAQAAAQQKPATATELSAKETVGAAFLRTLDDEAYELRSAAEFTPAEKYNYRP
                     TPGDYGEVYLGLGPKELRTFGEQIKHVACANFAFSAELDGKTPPAACDTGGPSPAKSR
                     DELVGYLRDSFKALRKSLGAITQKNMFEPIEGPYAGPNTRLGLANVAIWHCADHYGQL
                     VLYMRLNGIVPPASRAKPPELKD"
     gene            1004..1738
                     /locus_tag="VFR42_10615"
     CDS             1004..1738
                     /locus_tag="VFR42_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369655.1"
                     /translation="MARPLRQTKARKSPIQAVQMQYKILLMISTRNLAELPTIETLRK
                     LTQSLAMLDAILMREWDYRYYSFNSKWAENSQVGSMRNGQGDGWYCGFGPQGALLKGF
                     DHESEMSPWSMEIPKVWPGVLDAVPEVFKPFATEPAFSMEDTTFCIWRGIGDLHWSTG
                     EVSFPSSDDPDGSEWMLSILEGDSRTYKAWAEDYYECSISLPAVQQIYEHTPLTQELA
                     QELNKTAKFESLLADAIEINYPVGKS"
     gene            1829..2044
                     /locus_tag="VFR42_10620"
     CDS             1829..2044
                     /locus_tag="VFR42_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369656.1"
                     /translation="MNSKLRFASGISVLFTTLAFTHIMFHDTVHFLHGPESHWFVGIH
                     TVVAIVLIILSFTGAYYILTSGRKPSA"
BASE COUNT          448 a          626 c          616 g          435 t
ORIGIN      
        1 tgaagccgta cgcgcgccga tcgaaaagct cggcgggcgc attcacaatg cgtttttcgc
       61 gttcggtgag ttcgatgtgg tggtcatcac ggaaatgccc gacaacgtca gcgccgcggc
      121 catcgccatc gcgtttgccg ccggcggctc gatcaagaat gagaagacta ccccgctgat
      181 gaccacgacg gaagcgctcg aagctataag gaaggctggc accagcggct accgttcggt
      241 gaccgctccg agcagcgccg ccgctgcccg gacgtgagat tgccgagtgc ctacaaatcc
      301 gaacagtcgg ggcgcagcga tcgctgcgcc cctttcatcg aggttgccaa cgtgacgcgt
      361 tttgactgga tttgcatgtg tgttaccggg gcgctgctgt ttctgttttg cgcccatccg
      421 cacgcccaag ccgccgcgca acagaagccg gcgacggcga cggagcttag cgccaaggaa
      481 accgtggggg cggcgtttct gcgcacgctc gatgatgaag cgtacgagct gcgctcggcg
      541 gccgaattca cgcccgccga aaagtacaac taccggccga cgccgggcga ctacggcgag
      601 gtttatctcg gcttagggcc aaaagagctg cgcacgttcg gcgagcagat caagcacgta
      661 gcctgcgcca atttcgcgtt ttccgcggag ctcgacggca agacgccgcc agccgcttgc
      721 gacaccggcg gccccagccc ggcgaagtcg cgcgatgaat tggtggggta tctgcgcgat
      781 tcgttcaagg cgctgcgcaa atcgctgggc gcgatcacgc agaaaaacat gtttgaaccc
      841 atcgagggtc cgtacgccgg gcccaacacg cgcctgggcc tggccaacgt ggccatctgg
      901 cattgcgctg accattacgg acagcttgtg ctatatatgc ggctcaacgg catcgtgccg
      961 ccagccagcc gcgcgaagcc gccggagctg aaggattgag cgcgtggccc gccctttgcg
     1021 tcagactaag gctcgtaaat caccaatcca agctgtgcaa atgcagtata aaattttgct
     1081 gatgatctcg acgagaaacc tggctgaact gccaacgata gagacactta gaaagttaac
     1141 ccagtctttg gcaatgcttg acgccatcct gatgagggaa tgggactatc gctattactc
     1201 gttcaacagc aagtgggccg agaactcgca ggtggggtcc atgcgaaacg gacaaggtga
     1261 tggttggtat tgcggcttcg gcccccaggg tgcgcttctg aaaggtttcg accacgagtc
     1321 tgagatgtca ccgtggagca tggaaatccc taaggtctgg cccggcgtgc tcgatgctgt
     1381 tccggaggtg ttcaaacctt tcgcaactga acctgccttc tcgatggaag atacgacttt
     1441 ctgcatttgg cgtggcattg gagaccttca ctggagcact ggagaagtct cttttccatc
     1501 ctcagacgac ccagacggtt ctgaatggat gctttcgatt ctcgaaggag actcccgcac
     1561 ttataaagcg tgggcggaag actattatga atgctccatt agcctgccgg cggtccaaca
     1621 gatttatgaa cacaccccgt tgacacaaga gctggcgcag gaattgaaca aaaccgcaaa
     1681 gtttgagtct ttgttggccg atgccataga gatcaactat cccgtgggca aatcctgatt
     1741 aacgcgcaca accaaggcac tacccatcac cgaggattct gcggaatcgc cggacgatgc
     1801 gctacaatgg cgcgccactg aggatcccat gaattccaag ttgcgcttcg ccagcggcat
     1861 ttccgtgctg ttcaccacgc tggcctttac ccacatcatg tttcacgaca cggtgcactt
     1921 tctgcacggc ccggagagcc attggttcgt cggcattcac accgtcgtgg ccatcgtgct
     1981 gattattctg tcgttcactg gcgcgtatta cattcttact agcgggcgca aaccaagcgc
     2041 ctagtgattc ggggcgctgc gctacaatgt gggcgtgacc tcgccttccc tcacgccgcg
     2101 gcttgccggc attcgcctgc atccg
//
LOCUS       DASTPM010000603         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_165760, whole genome shotgun sequence.
ACCESSION   DASTPM010000603 DASTPM010000000
VERSION     DASTPM010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165760"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VFR42_10625"
     CDS             complement(<1..1163)
                     /locus_tag="VFR42_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0369657.1"
                     /translation="MSRPLGLRTHLLLFFVFLLCAVAAKAGIFGTVRGIVHDVQHRPI
                     SGAHLLLQAKQSDWKREAITDDNGEFQMDAVPAGNYTMRITRDGFRDYVGDLAVVADS
                     APLRHFPLEIAGVSERVEVSESAQTLDTTSSSAPITLSRQTISETPGATRTNSLDLIT
                     DYTPGAYMVHDQLHVRGGHQVSWLVDGIPVPNTNIAANVGAQFEPKDMDVVEIGRGGY
                     SADYGDRIYGTFNVIPRSGFERDREIELLATYGSYHSTDSQISIGDHSDRFAYYASVS
                     ANRSDFGLMPPEPEALHDATNGVSGFTSLIFNASPTDQLRLAASARSDFFQIPNTSEQ
                     QQALIRDVQRERDAFLNFTWVHTFQPGVLLNVAPFIHWNHAAYDGSSPIPSLLP"
     gene            complement(1254..1652)
                     /locus_tag="VFR42_10630"
     CDS             complement(1254..1652)
                     /locus_tag="VFR42_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369658.1"
                     /translation="MARRTPALLCLFALLLFQAPVMGAAMLASGVCCTGDHCPIAAHH
                     HSSAKTEEAPSGCEHGTNGATKLQKCSMSCCQTTEQSAVHAQVFVLSPDVTPPAFAPL
                     SEAVSRLTYDEEIGSFSPLSPPPKSLSFLI"
     gene            complement(1825..2166)
                     /locus_tag="VFR42_10635"
     CDS             complement(1825..2166)
                     /locus_tag="VFR42_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369659.1"
                     /translation="MRKLASFAVLFGMFGLPAWATTETFNGVSVIDTNCSKKAAADPD
                     AHTRECALKCQASGYGIITKDHKYLKFDSAGNTKITEALKLSDKKDHLRVDVTGDVNG
                     DTLQVTSIKLL"
BASE COUNT          525 a          599 c          732 g          457 t
ORIGIN      
        1 ggcagcaagc tgggaatcgg cgagctgccg tcatacgccg catggttcca gtgaatgaaa
       61 ggcgcgacgt tcagcaacac gccgggctga aaggtgtgca cccaggtgaa attcagaaac
      121 gcatcgcgct cccgctgcac atcgcgaatg agcgcctgtt gctgctcgga agtattcgga
      181 atttgaaaga aatcggaacg agcggatgca gcgagccgca actgatccgt cgggcttgcg
      241 ttgaagatga gcgaagtaaa tccgctgacg ccgttggttg cgtcgtgcag tgcctccggc
      301 tccggtggca tcaagccgaa gtcggaacga ttggcgctga cgctggcgta gtaggcgaag
      361 cggtcgctat ggtcgccgat gctgatctga ctatcggtgg agtggtagct gccgtaagtg
      421 gcgagcagtt cgatttcacg gtcgcgctca aaaccggaac gcggaatcac gttgaacgtg
      481 ccatagatgc ggtcgccgta atctgcggaa tatccgccgc gcccgatctc cacaacatcc
      541 atatccttgg gctcaaactg cgcgccgaca ttcgcggcaa tattggtgtt gggaacggga
      601 atcccgtcca cgagccacga aacctggtgg ccgccgcgta catggagttg gtcatgaacc
      661 atgtaggcac cgggagtgta gtcggtgatg aggtcgaggc tattcgtgcg agtggctccg
      721 ggtgtttcgg aaatagtctg gcgactcagc gtaatcggag cggaagagga ggtggtgtcg
      781 agcgtttgcg cggactcgct gacttcgaca cgttcggaaa cgccggcaat ttcgagcggg
      841 aagtgacgca ggggagcaga gtcggcgacc acggcgaggt caccgacata gtcacgaaag
      901 ccgtcacgcg tgatgcgcat ggtgtaattg ccggcgggaa cggcatccat ctgaaactcg
      961 ccgttgtcgt cggtaattgc ttcgcgtttc cagtcggatt gcttggcttg cagcaaaaga
     1021 tgcgcgcccg agatgggccg atgctgcaca tcatgcacga tgccgcgaac ggtgccaaag
     1081 attccggcct tggcggcaac ggcgcagagc aaaaaaacaa aaaataagag caaatgcgtg
     1141 cgtaatccca aaggacgtga cattgcaggt gatcggctcc cagaaaattt gagctgcgcc
     1201 gccggatcaa gcgggcaggc ggagttattc aggactacgc gagtagcagc agttcagatg
     1261 aggaaagaga gcgatttggg aggaggagag agaggagaaa acgatccgat ctcttcgtcg
     1321 taggtgaggc gagaaacagc ttcggagaga ggtgcgaaag cagggggcgt tacgtccggc
     1381 gacaacacaa agacctgagc gtggacggcg gattgttcgg tggtctggca gcaagacatg
     1441 gagcacttct gcaatttggt agcaccgttc gtgccgtgtt cgcaaccgct cggcgcttct
     1501 tcggtcttgg cggaagaatg atgatgcgca gcgatcgggc agtggtcacc ggtgcagcac
     1561 accccactgg ccagcatagc cgcgcccatc accggggcct gaaacaagag cagcgcgaag
     1621 aggcagagga gcgccggcgt ccgccgggcc actgcattcg cgaaattgcc agtgcacgtc
     1681 atatccgtca aagggtccgt tcacggcgga cactacgctc atatgatacg tcaaaattcg
     1741 cgttatggat ggcagccgcc gagggcacgg ccaggagtgc ccccagatcg actgttcgag
     1801 cgcgaacaac cagcggtgcg gaaattacag cagcttgatc gaggtgacct gcagggtgtc
     1861 cccgtttacg tcgccagtca catccacgcg cagatgatcc tttttgtctg aaagcttgag
     1921 agcttctgta atttttgtat ttcccgcgga atcgaatttc aggtacttgt ggtccttggt
     1981 gatgatgccg tatccgctgg cctggcattt gagcgcacat tcgcgggtgt gcgcgtcggg
     2041 gtcggcggcg gctttcttgg agcagtttgt atcaatcacc gaaacgccgt taaaagtttc
     2101 ggtggtggcc caagctggga gtccaaacat tccaaacaag acagcaaatg aagcaagctt
     2161 acgcattaca tcctccattt tttcgaattg agttaccgat tcactgcaaa ctgggtgccc
     2221 gcggacgctt tgctctgctg gcccaccggg atctgctcac gaaacgcgga aatttgcgcc
     2281 agagcaaagg caccgatggc tatctcaacg tcg
//
LOCUS       DASTPM010000604         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_483168, whole genome shotgun sequence.
ACCESSION   DASTPM010000604 DASTPM010000000
VERSION     DASTPM010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483168"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /gene="tsaD"
                     /locus_tag="VFR42_10640"
     CDS             complement(<1..550)
                     /gene="tsaD"
                     /locus_tag="VFR42_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU0369660.1"
                     /translation="MPENYILGIETSCDETAAAVVRDGQMLLSNVVSSQIATHQPYGG
                     VVPELASREHLRAIVPVVRQALAEAQQTYESVDAIAVTQGPGLAGALLVGISYAKSLA
                     FALNKPLITVNHLEGHIHAVLLEEKQKGNFQIDFPLLALVVSGGHTHLFLAEQRDRGW
                     TYKNIGRTRDDAAGEAYDKVAKL"
     gene            563..1951
                     /locus_tag="VFR42_10645"
     CDS             563..1951
                     /locus_tag="VFR42_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU0369661.1"
                     /translation="MGLQAIGRQKALNLSSEKAQEATRIALALRSQGHSAYLVGGCVR
                     DLLLQREPADFDVATSATPQQVMQLFPQTYAVGAQFGVVLVPVQRDSNGTPDNYAIEV
                     ATFRSDGAYSDGRHPDHVQFSTDARQDVQRRDFTINGLLLDPQTAEVLDFVGGRDDLK
                     RGVVRTIGEPRDRFVEDKLRMLRAVRFAARFGYVIDEQTFAAIRELAPQIHQVSRERV
                     RDEILKMLTEGHARRAFELLDEARLLEQVLPEIKKMQGVAQPPQYHPEGDVWVHTLML
                     LEGLPAGCSRTLALGALLHDVGKPPTFRVAPDRIRFDSHAEVGTKMAEEICRRFRLSN
                     EDTGQVLSLVANHMRFGDVMRMKDSTLKRFFRLPKFEEQLELHRLDCQSSHRDLSLYE
                     FAKEKYHHLPAEQIRPEPLITGADLIAAGYQPGPQFKELLTAVEDAQLDGSISTREEA
                     MDLVRAKVRELA"
     gene            complement(2153..>2387)
                     /locus_tag="VFR42_10650"
     CDS             complement(2153..>2387)
                     /locus_tag="VFR42_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369662.1"
                     /translation="IPRFMLDDVETPHKTGDFPPYIANDVGLIETPSARVVVVFFSAH
                     HRGYYQELEDAIGRMSEQVWGYFNYREKAPAAK"
BASE COUNT          472 a          765 c          663 g          487 t
ORIGIN      
        1 gcagcttggc gaccttatcg taagcttcgc cggcagcatc atcgcgcgtg cggccgatgt
       61 tcttgtaagt ccagccgcga tcacgctgct ccgccagaaa aagatgtgtg tggccgcccg
      121 acaccaccag cgccagcagc ggaaagtcga tctggaaatt ccctttctgc ttctcttcca
      181 gcagcaccgc atggatgtgc ccttccagat ggtttaccgt gatgagaggc ttgttgaggg
      241 caaaggcgag cgacttggca taactgattc ccaccagcaa cgctccggcc aggcccggcc
      301 cctgcgtgac cgcgatcgca tccaccgact cataagtctg ctgtgcctct gccagcgcct
      361 gccgtactac cggaacaatc gcgcgcagat gttcccgcga ggcgagttcc ggcaccacgc
      421 cgccataagg ctggtgcgtg gcgatctgtg atgagactac gttcgagagc agcatctggc
      481 cgtcacgcac tacggccgcg gcggtctcgt cacatgaggt ttcaatcccc agaatgtaat
      541 tctccggcat atatctaata ttatgggatt gcaggccatc ggccggcaaa aagcgttgaa
      601 tctctcctct gaaaaagcgc aggaagccac ccgaatcgca ctcgccttgc gtagtcaggg
      661 ccattctgcc taccttgtgg gcggctgcgt gcgcgacttg cttctccaac gcgagcctgc
      721 cgacttcgac gtcgccacct ccgccacgcc tcaacaggtc atgcaactct ttccgcagac
      781 ctatgccgtg ggcgcgcaat tcggagtggt gctggttccg gtacaacgtg acagcaatgg
      841 caccccggac aactatgcca ttgaggtcgc cacctttcgc agcgacggcg catactcaga
      901 cggccgtcat cctgaccacg tgcagttctc taccgacgcg cgccaggacg tgcaacggcg
      961 cgatttcacc atcaacggcc tgctgcttga tccgcaaacg gccgaagtgc ttgatttcgt
     1021 cggcgggcgc gatgacctga agcgcggcgt ggtccgcacc atcggcgagc cccgtgaccg
     1081 gtttgtggaa gacaaactgc ggatgctgcg cgccgtgcgt tttgcggcgc gctttggcta
     1141 cgtgattgac gaacaaacct ttgccgccat ccgcgaactc gccccgcaga tccatcaggt
     1201 aagccgcgag cgcgtgcgcg atgagattct caagatgctc actgaaggcc acgcccgccg
     1261 cgcctttgag ttgctcgatg aggcgcgtct gctggaacag gtgctgccgg agatcaaaaa
     1321 aatgcagggc gtagcgcagc cgccgcaata tcaccctgaa ggcgatgtct gggtccacac
     1381 cctgatgctg ctggaaggcc tgccggccgg ctgctccaga actctggccc tcggcgcgct
     1441 gctgcacgac gtaggcaagc cgcctacgtt tcgcgtggcg ccggaccgca tccgctttga
     1501 ctcccatgcc gaggtcggca cgaaaatggc ggaagaaatc tgccgtcgct ttcgcttgtc
     1561 caacgaagac accggccagg tgctgtcgct ggtcgcaaac cacatgcgct ttggcgacgt
     1621 gatgcgcatg aaagactcca ccctgaagcg tttttttcgc ctgccgaaat tcgaagagca
     1681 gctcgaactg catcggctgg attgccagag cagccatcgc gacctgagcc tgtatgaatt
     1741 cgcgaaggaa aaataccacc acctgcccgc cgagcagatc cgccctgaac cactgatcac
     1801 cggcgctgac ctgatcgccg ccggctatca acccggaccg cagttcaagg aattgctgac
     1861 tgccgtcgaa gacgcgcagt tggatggatc catcagcacc agggaagaag cgatggattt
     1921 ggtgcgggca aaagtcagag aacttgcctg accctccccc tcccacctca actaggattt
     1981 taaagagctt gcacaattca accccgacga tgctcgcgtg acactgcccc tcggcggtgg
     2041 ctcggccgct tcctcggttc ctatcagtgt caatcttttt tcatcagcgg tgagactttg
     2101 tcttctctct gtgcctccgt gtctccgtgg tggattttcc tgcacgcggc tttcacttgg
     2161 ctgcaggagc tttctcccga taattgaaat acccccacac ctgctcgctc atccttccta
     2221 tcgcatcttc cagctcctgg taatatccgc gatggtgcgc cgagaagaac accaccacca
     2281 cgcgcgctga cggcgtctcg atcaggccca cgtcgttggc aatgtatgga ggaaagtcgc
     2341 cggtcttgtg cggtgtctcc acatcgtcca gcatgaagcg gggaatg
//
LOCUS       DASTPM010000605         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1348041, whole genome shotgun sequence.
ACCESSION   DASTPM010000605 DASTPM010000000
VERSION     DASTPM010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348041"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFR42_10655"
     CDS             <1..701
                     /locus_tag="VFR42_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369663.1"
                     /translation="AVPESTGCLSPRHPRLEIATDSSSYREAVDAMQYVSVFMGGTGL
                     MLIVRAVKWQFTTVSGARTLRIFPEMKLRNVVPLQRHRPMALFSAPLDFGIAWGSVLM
                     VVAFVFYRGLLMHPPAQGLRISFSKGKVAQAFESPWKESLGVYIDRRGRFLLNGQVVE
                     HESLPVKLQEKLGKRMVWTVYFEADNASLFRETAYAIDKIQGLGAKVIWITPKMRDEW
                     RAAAGWPSPETAAP"
     gene            complement(764..1228)
                     /locus_tag="VFR42_10660"
     CDS             complement(764..1228)
                     /locus_tag="VFR42_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0369664.1"
                     /translation="MKKSLTLLLAALALCAIVFAQNKLAPSPGSANDEIQIRQLERAW
                     NEAEARHDAKAVTNIVGDTLTYIDFDGSIMNKDEYIQDVTKTAYQADHLYDEGLTVTV
                     YGNAAVVTGIYRETGTTKGKSYVHRVRFTDTWIKQSGVWRCVASQSTLIQSK"
     gene            1401..2462
                     /locus_tag="VFR42_10665"
     CDS             1401..2462
                     /locus_tag="VFR42_10665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0369665.1"
                     /translation="MATNSTTVKAQRGTKLRRKLRFLRTRARELRMIGKALASTKHPI
                     LVHIIPMRRCNLDCGYCNEYDQVSKPVPLEEMKRRLDYLAGMGTTIITISGGEPLMHP
                     QLEEVIRHIRKHGMIAGMITNGFLLSKERIASLNAAGLEHLQISIDNVTPDEVSKKSL
                     KTLDSRLEWLSEHAIFQVNINSVLGSGVKNPEDALTIAHRAIELGFTSTVGIIHDGMG
                     QLKALSGRQIEIFEEIMTLGKRSFSRFNDFQHSVARGKEHNWRCRSGSRYLYICEDGL
                     VHWCSQQRGYPGIPLAQYTPEMRHREYLTDKFCGPRCTVSCVQQVGILDNWRDPQTLK
                     PMPMATAPTPASELVQIGK"
     gene            2546..3505
                     /locus_tag="VFR42_10670"
     CDS             2546..3505
                     /locus_tag="VFR42_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0369666.1"
                     /translation="MPVHAIETENLSKEYPHGFLHLKKKTSLEGLTMQVEDGEVFGLL
                     GPNGAGKSTTIKLLMGIIFPTAGCARMLGKPVSDVSMHRDIGYLPEQPYFYDYLTATE
                     VLDYFARFHGYSAAERGERVQKMLKKVGLETAGKIQLRKYSKGMLQRVGLAQAILHDP
                     KLVILDEPMSGLDPVGRREVRDVILELKNAGKTILFSTHILPDAETLCDRVGVIAGGK
                     LRGVGAPGAIVGVKATGMEILFELAEGAALPEAIRAQVTKSGAGYRVSVEESELYTAL
                     EGLRAAGARITSVAQIRPTLEDFFMELVGRDRAQAAAIEVSTQ"
BASE COUNT          787 a          809 c         1239 g          721 t
ORIGIN      
        1 tggcagtacc agagtccacg ggttgcctga gcccgagaca tccacggctg gaaatcgcga
       61 cggattcgag tagctaccga gaagcggtag atgcgatgca atatgtgagc gtgtttatgg
      121 gcggcacagg gctgatgctg attgttcgcg cggtgaaatg gcagttcaca acggtgtctg
      181 gagcaagaac gcttcgaata ttcccagaaa tgaaattacg aaatgttgta ccgctgcagc
      241 gacatcgtcc gatggcgctg ttctcggcgc ctttagactt cggaatcgcc tggggcagtg
      301 tcttgatggt ggtggcattt gtcttttatc gagggctgct gatgcatcct cctgcacaag
      361 gtttgcgaat tagtttttcg aaggggaagg tagctcaggc cttcgagagt ccgtggaagg
      421 aatcactagg cgtctacatt gatcgcaggg gacggttcct cttgaacgga caggttgtgg
      481 aacacgagtc gttaccggtc aaactgcagg aaaaacttgg gaagagaatg gtttggacgg
      541 tctatttcga agctgacaac gcgagtctgt tccgagaaac cgcgtatgca atcgacaaga
      601 ttcaggggct gggagcgaag gtcatttgga taacgccaaa gatgcgggat gaatggaggg
      661 cggctgcagg ctggccttcc ccggaaaccg ctgcaccctg aagcagctca gcgcgtggca
      721 ccgaaggggt tcagaacaat agtcaaagtg cctgtaacac ttcctacttg gattgaatga
      781 gggtgctttg gctggcgaca cagcgccaga cgccgctttg tttgatccaa gtgtcggtga
      841 aacggacgcg gtggacgtag ctcttgcctt tggtggttcc ggtttcacgg taaattccgg
      901 tgacaacggc ggcgttgccg tagacggtga cggtgagtcc ttcgtcgtaa agatggtcgg
      961 cctggtaggc ggtcttggtg acatcctgga tgtactcgtc tttgttcatg atcgagccgt
     1021 cgaagtcaat ataggtgagc gtatcgccga caatgttggt gacggctttg gcatcgtggc
     1081 gggcttcggc ttcgttccag gcgcgctcga gctggcgaat ctggatttcg tcattggccg
     1141 aaccggggct aggagccagc ttgttttgtg cgaagacgat agcgcagaga gcgagcgcgg
     1201 cgagcagcag ggtgagcgac tttttcattg cagccacctt aagtaggggg aattgcgcgt
     1261 agcgtagcag gcgtggagcg cgggtcgtca ggaaaattga gggtatgaca cgtttcaggc
     1321 acagggtgca cagagcggag tggcagggcg gttgagtcgg gtataattga agttcgggga
     1381 aacgcatcca aaactctgga atggcaacga acagcacaac ggtgaaggcg cagcgcggga
     1441 cgaagctgcg gcgaaagctg cggtttttgc ggacccgtgc gcgtgagctg cggatgatcg
     1501 ggaaggcgct ggcgtcgacc aagcacccaa tcctggtgca catcattccg atgcggcgct
     1561 gcaacctgga ctgcggctat tgcaacgagt acgaccaggt gtcgaagccg gtgccgctgg
     1621 aggagatgaa gcggcggctg gactatctgg cggggatggg gacgacgatt attacgatca
     1681 gcgggggcga gccgctgatg catccgcagc tggaagaggt gatccggcac atccgcaagc
     1741 acgggatgat tgccgggatg atcacgaatg gatttttgct gagcaaggaa cggattgcga
     1801 gcctgaacgc ggcggggctg gagcacctgc aaatcagtat tgataacgtg acgccggatg
     1861 aggtgtcgaa gaagagtttg aagacactgg attcgcggct ggagtggctc tcggagcatg
     1921 cgatttttca ggtgaacatc aactcggtgc tgggcagtgg cgtgaagaac cccgaggatg
     1981 cgctgacgat cgcgcaccgc gcaattgaac tggggtttac aagcacggtg ggcattattc
     2041 acgatggcat ggggcagttg aaagcgctga gcgggcggca aatcgaaatc tttgaagaga
     2101 tcatgacgct ggggaagcga tcgttttcgc ggtttaacga ttttcagcac agcgtggcgc
     2161 gcgggaaaga gcacaattgg cggtgccgtt cgggctcgcg gtatttgtac atttgcgagg
     2221 acgggctggt gcactggtgc tcacagcagc gcgggtatcc gggaattccg ctggcgcagt
     2281 acacaccgga gatgcggcac cgggaatatt tgacggacaa gttttgcggg ccgcggtgca
     2341 cggtgtcatg cgtgcagcag gtggggattc tggataactg gcgggatccg cagactttga
     2401 agccgatgcc aatggcgact gcgccgaccc cggcaagcga actggtgcag atcggcaagt
     2461 aacgacaaat cgccgagttg cagtttgtcg cacaacgtgg cgcaaggcgc gttgacgcgg
     2521 cgggcaggct tgccagaata gaagcatgcc ggttcacgcg attgaaacag aaaacctttc
     2581 caaagaatat ccgcacggat ttctccatct gaaaaagaaa acctcgctgg aaggcctgac
     2641 catgcaggtt gaggacggcg aagtgtttgg gctgctgggg ccgaacggcg cggggaagag
     2701 caccaccatc aaactgctga tggggattat ttttcctacg gcggggtgcg cgcgaatgct
     2761 cggcaagccg gtgagcgatg tgagtatgca ccgcgacatc ggctatttgc cggagcagcc
     2821 gtatttctat gactacctga cggcgacgga agtgctggat tactttgcgc gattccatgg
     2881 gtatagcgcg gcggagcgcg gcgagcgtgt acaaaaaatg ctgaagaagg tggggctgga
     2941 gacggcgggg aaaattcagc tgcggaaata ttcgaaggga atgctgcagc gcgtggggct
     3001 ggcgcaagcg attctgcacg atccgaagct ggtaattctg gacgagccaa tgtcggggct
     3061 ggacccggtg ggtcggcggg aagtgcgcga cgtcattctg gaattgaaga atgcaggaaa
     3121 gacgattttg ttttcgacgc atattttgcc ggacgcggag acgttgtgcg atcgggtggg
     3181 tgtgattgcg ggagggaagt tgcgcggagt aggagcgccg ggcgcgatcg tcggagtgaa
     3241 agcgacgggg atggagattt tgttcgaact ggcggaagga gcagcgttgc cggaggcgat
     3301 tcgcgcacag gtgacgaagt cgggtgccgg ctaccgagtc agcgtagaag agagcgaact
     3361 gtacacggcg ttggaagggt tgcgcgcggc gggagcacga attacatccg tggcgcaaat
     3421 caggccaacc ttggaagatt ttttcatgga gctggtgggc agagatcgcg cgcaggcagc
     3481 ggccatcgag gtgagcaccc aatgagcagg gtcttgatcg tcgcgcgcaa cacgttccgg
     3541 gaagcagtgc gcgacc
//
LOCUS       DASTPM010000606         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_21964, whole genome shotgun sequence.
ACCESSION   DASTPM010000606 DASTPM010000000
VERSION     DASTPM010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21964"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VFR42_10675"
     CDS             complement(<1..953)
                     /locus_tag="VFR42_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369667.1"
                     /translation="MARTAQDVLEFDKLRELLRLRTTCVLGRRALDALEFSTDRASLE
                     AAFAAIREAREWLRTGNELGFGGLADPQQWLEHIEGPGVVLDAAELLDAASLLETAGA
                     LRQQFREQAASFPLVSAKATSLADFRDALAAIRRCVLPNREISDDASSALRRIRASIG
                     QTRDSIQRTLKQILRSRNAEAGEDYVTLRNDRFVIPVRAEHRRSVPGIVHGTSGTGQT
                     VFLEPFETVETNNQLVQLAEEEAAEIVRILRELTERLQLMRGPLVAAADTIAELDSVF
                     ARARFARDFDAAIPELAHESELRLDSARHPVLEDKLRRENRA"
     gene            complement(960..1505)
                     /locus_tag="VFR42_10680"
     CDS             complement(960..1505)
                     /locus_tag="VFR42_10680"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEU0369668.1"
                     /translation="MRERIITVFGSSRPRESDAEYTEARDLGQTLAANGFSVCSGAYA
                     GVMAAVSRGAKEAGGKTYGVTADFFSAPANPWIDVEVRVSTWQERLFELIRLADGFVA
                     CKGGTGTLVELAVVWEMLNKSVMSGKPLAVLGNFWTPIIECVRSVEVAHSSPWGEASQ
                     TLIYVAATPKDAAVHLRKKLE"
     gene            complement(1505..>2006)
                     /locus_tag="VFR42_10685"
     CDS             complement(1505..>2006)
                     /locus_tag="VFR42_10685"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU0369669.1"
                     /translation="ASARSVAVWKTPEDLMKFPQDFYAAAGIAPGDVIQSDASGLSRH
                     DLVTPRAIGALLKYAQTQPWFAPYYASLPVAGVDGTLETLMKNTISAGHIHAKTGSVE
                     HVRTRSGYADTLGGRRLIFSFMSNNQGGKNHEASDALDGLCLAMMEEFDRKAAPPHEH
                     KGQRAH"
BASE COUNT          371 a          674 c          553 g          408 t
ORIGIN      
        1 gcgcgattct cgcgcctcag tttgtcttcc agcaccggat ggcgcgcgga gtccaggcgc
       61 agttcgcttt cgtgggcgag ctcaggaatg gcagcatcaa agtctcgcgc gaaccgcgcc
      121 ctcgcaaaca cgctgtcgag ttcagcgatg gtatccgccg cggcaaccag cggcccgcgc
      181 atcaactgca agcgctccgt caactcgcgc aggatccgca caatctccgc ggcttcttct
      241 tccgcaagtt gcacgagctg attgttggtt tcgacggtct cgaaaggctc caggaaaacc
      301 gtctgcccgg tcccgcttgt cccatgaact atgcccggca cgctgcgccg gtgttccgcg
      361 cgcaccggta tcacgaagcg gtcattgcgc agggtgacgt agtcttcgcc cgcctcggca
      421 tttcgcgatc gcaggatttg cttgagcgtc ctttgaatcg agtcgcgcgt ctggccgatg
      481 ctcgcgcgga ttctccgcag agcggaagac gcatcgtcac tgatctctcg attgggcagc
      541 acgcagcgtc gtatcgccgc cagagcgtcg cgaaaatcag cgagggatgt cgccttcgcc
      601 gaaaccaggg gaaagctcgc ggcttgctcc cgaaattgct ggcgaagtgc gcctgccgtt
      661 tccaagagcg atgccgcgtc cagcagttcc gcggcgtcta gcacgacccc cgggccttcg
      721 atgtgctcga gccactgctg cgggtcagcg agtccgccaa agccaagttc atttccggta
      781 cgcaaccact cccgcgcttc ccgaattgcg gcgaacgccg cttccaatga cgcccggtcc
      841 gtgctgaact ccagcgcatc cagtgcccgc ctgccaagca cacacgtagt gcgcagccgc
      901 aacaattccc tcagcttgtc aaattccaaa acgtcttgcg ctgttcgtgc catttctctt
      961 tactctaatt tctttcttag atgaaccgca gcgtctttgg gcgttgcggc tacgtaaatc
     1021 agcgtctggc tggcctcgcc ccaaggcgag ctatgggcca cttcgacgga gcgcacgcat
     1081 tcgataattg gcgtccagaa attccccaac accgctagtg gctttccact catgaccgat
     1141 ttattgagca tctcccaaac tacggccagt tccacgaggg tgccggtgcc gcccttgcag
     1201 gccacgaacc cgtccgccaa ccggatcaat tcaaagagcc gctcttgcca ggtcgaaact
     1261 cggacctcca cgtcgatcca cggattggca ggcgcgctga aaaagtccgc ggttacgccg
     1321 taggtcttgc cgccagcctc cttggcgccg cgggagacgg cggccataac gcccgcatac
     1381 gcaccgctac agactgaaaa tccattggcg gccaacgtct ggcccaggtc gcgagcctct
     1441 gtgtactcgg cgtcactctc acgcggccgg gagcttccaa acacggtgat gatgcgttct
     1501 ctcatcaatg tgcacgctgg cccttgtgtt cgtgcggtgg cgccgccttc cgatcgaatt
     1561 cctccatcat cgccaggcac agtccatcca gcgcatccga ggcttcgtga ttctttccac
     1621 cctggttgtt gctcataaaa gaaaagatca gccggcgccc gcccaaagta tccgcgtatc
     1681 cggagcgcgt ccggacgtgt tccacggagc ccgttttcgc atgaatgtgc cccgcgctga
     1741 ttgtattctt catgagcgtc tccagcgtgc cgtcgactcc ggcgactggc agcgacgcat
     1801 agtacggcgc aaaccacggt tgcgtctgcg cgtatttcaa cagcgcccct atcgctcgcg
     1861 gcgtcaccaa atcgtgccgc gaaagcccag aggcgtcgga ctgaattacg tcacccggcg
     1921 cgattcccgc cgccgcataa aaatcttgcg gaaacttcat aaggtcctcg ggcgttttcc
     1981 agaccgcgac gctgcgggct gacgcc
//
LOCUS       DASTPM010000607         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_483357, whole genome shotgun sequence.
ACCESSION   DASTPM010000607 DASTPM010000000
VERSION     DASTPM010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483357"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VFR42_10690"
     CDS             <1..1149
                     /locus_tag="VFR42_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU0369670.1"
                     /translation="VIVGTIEVDDMELLTLSSKPGVDPLDWEIHNLKLHEVGADKPFG
                     FEGTLTNAKPKGEIATAGEFGPWDGDDPGATPVSGVYGFDEADLGPFPGIAGILSSTG
                     KYRGELDRLEVNGETDTPDFSLDNAGKPFPLHTDYSATVDGTNGDTELHPVHAILGKS
                     EIVASGSVLSVKGQGHQISLDVTTPNARIEDILQLAIKSDQPFLRGPIRVTAKLSLPP
                     GKQKVIDKMDLNGTFAITDGRWASPEMREKLESFSRHAEGKPEDDQAGSAVTDLKGRF
                     VLKNSVITFSKLAFSVPGAGVELAGTYEIHSQKIDMQGHLRMQAKLSQTVTGAKSFFL
                     KAIDPFFAKNGAGTELPITITGTQENPVFGVSVFHKKIEKQMGNTGAK"
     gene            complement(1166..2710)
                     /locus_tag="VFR42_10695"
     CDS             complement(1166..2710)
                     /locus_tag="VFR42_10695"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU0369671.1"
                     /translation="MFRSRRRFLATLAAAPFAKFRVAKDGSSPDLESAEDLQARLAAD
                     PLRPQCHLLPAKNWMNDPNGPIFWQGQYHMFFQYNPNAAVWGDMHWAHAVSPDMIHWR
                     HMPVALAPTPGWDDADGCFTGSAVDDNGTATILYTGVKSVAAERATLRDGANNFREVQ
                     CLATSTDPQLRSWTKWKTPVIQPPEDPLLTGFRDPFLWRDRDVWYLGVGSGKRRQGGQ
                     VLLYRSNDLRQWEYLHPLATGNWTERENTNPVESGEMWECPDFFALGRKFVLLYSTAG
                     KVVWEVGELDSKELVFHPQRSGILDHGAYYAQKTQSGQPLLWGWIPEKRPQAEYSAVG
                     WAGCMALPRRLTLSQEGDLRMDVATQAESLRVKAFNTNSELAKSSADERIREIQKIEI
                     NSVAGEFWWNTNTLPFAVTIEDNAGPWWALRAEPSGSSSLKLQINEKAIDVSGENHQG
                     MECRLFLDASVAEFFCNRLHVVTSRIYRRPAGLLRVRLTQADAKTLRSFEAWQLRAIS
                     GDRLTT"
     gene            complement(2740..4212)
                     /gene="gltX"
                     /locus_tag="VFR42_10700"
     CDS             complement(2740..4212)
                     /gene="gltX"
                     /locus_tag="VFR42_10700"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU0369672.1"
                     /translation="MSEHSGGSQSGVRVRFAPSPTGYLHVGGARTALFNWLFARRERG
                     TMVLRIEDTDAERNKPELVTGIIDGLKWLGVDWDEGPFYQSQRTAMYQEAGKKCLASG
                     AAFLCYCPPEKYVGGDHAVEGAETQLRRITRCSCREGKPSTTGVKPAIRFKVPVGGTT
                     KFLDAVFGEREFSNDEIEDFVLVRSGKGEEEFGQAMYQLSAVVDDIDMRITHVIRGAD
                     HISNTPKQVLLYRALGAEIPIFAHLPLILGADKSRLSKRHGATDVNMYRAEGFLPEAF
                     RNFLALLGWSPGGDEEFLRTSQLLEKFTLAGVSRTNAVFDRPKLEWFNTQYLQKLPIE
                     ELLPEVEEQLKSSKLWKEEWARGAGRAWFAKTVDLLRPRVRLLPDFSSWSRAFFSDDF
                     VRDPAAKEKFWKEPARIEQLLGKTADALAKVTDWSHDPCDHATRAVAEAAGVKAGLLI
                     NAIRVAIVGQAVAPPLFDTMVVLGQERVVRRLRDACAELG"
BASE COUNT          968 a         1376 c         1240 g          918 t
ORIGIN      
        1 gtgattgtgg gaaccataga agttgacgac atggaattgc tgacactttc cagcaagccc
       61 ggtgtcgatc cgctggactg ggaaatccat aacctcaaac tgcacgaagt cggagcagac
      121 aagccgtttg gattcgaagg aactctgacc aacgctaaac ccaagggcga aattgcgacg
      181 gccggcgagt ttggtccgtg ggacggcgat gaccccggcg caactcccgt ttccggggtc
      241 tatgggtttg acgaggctga cttgggaccg tttccgggaa tcgcgggtat cctatcatcc
      301 accggaaaat ataggggcga gctggatcgt ttggaagtga acggcgaaac cgatacgccg
      361 gatttctcgt tggacaatgc cggcaagccg tttccgctgc acaccgacta ttcggcaacc
      421 gtggacggca cgaacggcga cacggaactg catcctgtcc acgccattct gggcaaatcg
      481 gagattgtgg cgtccggcag cgtgctcagc gttaaggggc aaggccatca gatttccctc
      541 gacgtgacca cgccgaacgc ccgcatcgaa gatatcttgc agctggcgat caagtcggat
      601 caaccctttt tgcgggggcc gattcgcgtc acggccaagc tctctctgcc gcccggcaag
      661 caaaaggtca ttgacaaaat ggatttgaac gggaccttcg ccatcaccga cggtcgctgg
      721 gccagcccgg agatgcgcga gaagttggaa agcttcagcc gccacgccga aggcaagccg
      781 gaagatgacc aagcaggcag cgccgtgacc gatctgaagg gccgcttcgt actgaaaaac
      841 agcgtaatca cattttccaa gttggcgttc tctgtgccgg gcgccggcgt agagctcgcc
      901 ggaacttacg agattcacag ccagaagatc gatatgcagg gccacttgcg catgcaggcc
      961 aagctttccc agaccgtgac cggcgcaaag tcattttttc tgaaggcgat tgatccgttt
     1021 ttcgcgaaga acggcgcggg cacggaattg ccgattacca ttaccggcac gcaggaaaat
     1081 ccggtctttg gcgtctccgt gttccacaag aagattgaga agcaaatggg gaacacgggc
     1141 gccaagtaag tcgcggcgcc gcgccttagg tagtcagtcg atccccggaa attgccctta
     1201 gttgccacgc ctcaaacgat ctgagagttt tcgcgtccgc ttgggtcaag cgcacgcgaa
     1261 gcagcccggc tggccttcgg taaatccgag aagtcactac atgcaggcgg ttgcaaaaga
     1321 attctgccac ggaggcgtcc agaaacagcc ggcactccat tccctgatgg ttttcgccgg
     1381 aaacatcgat cgctttttcg tttatctgga gcttcaagga agaggatcca gacggttcag
     1441 ctcgaagcgc ccaccacggc ccggcgttgt cttcgatggt tacggcaaaa ggaagggtgt
     1501 tggtgttcca ccaaaactcg ccggcgacag agttaatttc aatcttctga atctctcgaa
     1561 ttcgttcgtc ggccgaactc ttggcaagtt cgctgttggt gttaaatgcc ttgacgcgca
     1621 acgattcagc ctgagtggcc acgtccattc tcagatcacc ctcttggctc aaggtcaggc
     1681 gtcgcggcag cgccatgcat cccgcccagc ccaccgcgct gtattccgcc tgcggacgct
     1741 tctccgggat ccagccccac aaaagcggtt ggccggattg cgttttctgc gcatagtaag
     1801 cgccgtgatc cagaatgccg ctgcgttgcg gatgaaacac caactccttc gagtccaact
     1861 cccccacttc ccagaccact ttgcccgccg tcgaatacag cagcacaaat tttctgccca
     1921 gtgcaaaaaa atccgggcac tcccacattt ctccggactc cacgggattc gtgttttcgc
     1981 gctcggtcca atttccggtc gctaggggat gcaagtattc ccactggcgc agatcgttcg
     2041 agcggtagag caagacttgg ccgccctgcc tgcgtttacc cgaaccgaca cccagatacc
     2101 atacatcgcg gtcgcgccat aagaatgggt cgcgaaaacc ggtaagcagc gggtcctccg
     2161 gcggctggat cacgggagtc ttccatttgg tccaggagcg caactgcgga tccgtggaag
     2221 tcgcaaggca ttgcacttcg cgaaaattat ttgctccatc gcggagcgtc gcgcgctccg
     2281 ccgcaacgct cttcacgccg gtgtaaagaa tcgtcgctgt cccgttgtcg tccaccgcgc
     2341 tgccggtgaa gcatccatcg gcgtcgtccc agcccggcgt cggcgcaagc gcaaccggca
     2401 tgtggcgcca gtgaatcatg tccggactca ccgcgtgcgc ccaatgcatg tcgccccaaa
     2461 ccgctgcatt agggttgtac tggaaaaaca tgtggtattg gccctgccag aagatggggc
     2521 cgttagggtc gttcatccag tttttggcgg gaagcaagtg gcattgcggc cgtagcggat
     2581 cggcggcaag acgcgcctgc aagtcttccg cgctctctag atcaggagaa gacccatctt
     2641 ttgcgacgcg aaattttgca aaaggcgctg ccgcaagcgt ggcgagaaac cttcggcgcg
     2701 aacgaaacat gcgaacctcg acggattacg gcagcgagtc tagcctagtt ctgcgcaggc
     2761 gtcgcgcagg cggcgcacca cgcgctcttg ccccaatacc accatcgtat cgaacaacgg
     2821 cggcgcaacg gcctggccca caatggccac gcgtattgcg ttgataagca aacccgcctt
     2881 tacgccggcc gcttcggcaa ctgcgcgagt ggcgtggtcg cagggatcat ggctccagtc
     2941 ggtaacttta gcgagagcat cggcagtctt gccaagcaac tgctcaattc tcgcgggctc
     3001 tttccaaaac ttttccttcg ccgcaggatc gcgtacgaaa tcgtcgctga aaaacgcgcg
     3061 cgaccacgaa gaaaaatcgg gcagcagccg aacacgcgga cgcagcaggt ccacggtctt
     3121 ggcaaaccat gcccggcccg ccccgcgggc ccactcttct ttccagagct tgctgctctt
     3181 gagctgctct tccacttcgg gcagcaactc ctcaatcggc agcttttgca ggtactgcgt
     3241 gttgaaccac tccagcttgg gacgatcgaa cacggcgttg gtgcggctga cgcctgccag
     3301 ggtgaatttc tccagcaact ggctggtacg caagaactct tcgtctccgc cgggcgacca
     3361 tccaagcagc gccaggaaat tgcggaatgc ctccggcaaa aacccttctg cgcgatacat
     3421 gttcacgtcg gtcgcgccgt gacgtttcga gagccgcgat ttgtcggcgc caagaatcaa
     3481 cggcaagtgc gcaaaaatcg gaatttcagc gcccagcgcg cggtacagca aaacttgttt
     3541 gggcgtattg gaaatatggt cggcgccgcg aattacatgc gtgatgcgca tgtcgatatc
     3601 atccaccact gcactgagtt gatacatggc ctggccaaac tcttcttcgc ccttgcccga
     3661 acgcaccagc acgaaatcct cgatttcatc attcgagaat tcgcgctcgc caaacacggc
     3721 gtccaggaac ttggtagttc cgcccactgg tactttgaaa cgaatcgctg gcttgacccc
     3781 tgtcgtggaa ggttttcctt cgcggcacga acagcgcgta atgcgccgca gctgcgtttc
     3841 cgcaccttcc acggcatgat cgccgcccac atatttttcc ggcgggcaat agcacagaaa
     3901 cgccgcgccg ctggccaggc acttctttcc cgcttcctga tacatcgccg tgcgctgcga
     3961 ctgataaaac ggcccttcat cccaatccac gcccagccat ttcaatccgt cgataatccc
     4021 cgttaccaac tccggcttgt tgcgctcggc gtcggtgtcc tcaattcgca aaaccatggt
     4081 gccgcgctcg cgccgcgcga aaagccaatt gaacaaagca gtgcgcgcgc ccccgacgtg
     4141 gagatagccg gtgggagaag gtgcaaaacg cacccggacg ccgctctgcg aaccgcctga
     4201 atgttcggac atgaaccgtt ctgacccttt ggaaaaatta cccttgggaa cgctgtaagt
     4261 tacagtgtaa aggaacctta cgggagcgtc caacgtccct ccaaaccact gctatggttt
     4321 ggcctatccc tggcacgatt tcgccctgct gtcacttacg caaccagtac gccggtacgc
     4381 ttgcgcaccc aattttccct gcaccataat cgcaagtact ttgttttgtg cagagccggt
     4441 cggtttggtc acccccacga gtcctcgcgt gagcggcgat ctaccgactc ctggcgccat
     4501 tt
//
LOCUS       DASTPM010000608         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1290515, whole genome shotgun sequence.
ACCESSION   DASTPM010000608 DASTPM010000000
VERSION     DASTPM010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290515"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(865..1344)
                     /locus_tag="VFR42_10705"
     CDS             complement(865..1344)
                     /locus_tag="VFR42_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0369673.1"
                     /translation="MARILIADDSELVRVSLKALFADHQDLQVCGEASDGWQAIQLAT
                     QLKPDLLILDIAMPVLDGFHAAAEIVKREPTLPIIIYSLHESPQVELEAKKAGARCVV
                     SKSASPELLLKAIREATAQQSAKSVPDVLLGAPAPSDATVELSLEAKSESDGQAELN"
     gene            1757..2398
                     /locus_tag="VFR42_10710"
     CDS             1757..2398
                     /locus_tag="VFR42_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0369674.1"
                     /translation="MVRPHLALVAMPNHSVLQEPRAAINFAYFPNDGLASIVVAMKGE
                     RDVEAGVVGYEGAVGTALAVGLKTTPLRVVMQIEGKALRIPAGTLVSLLRRTPELQMR
                     LSRYAVLQGMQVAQTAACNRLHHVSRRLARWLLMAQDRVDSGTLEITHDFLATMLGTD
                     RPSVTLAARSLQAKKAIRYRRASVDIVNRKRLESLTCQCYGAVQQLNGRLGLD"
     gene            2583..>2917
                     /locus_tag="VFR42_10715"
     CDS             2583..>2917
                     /locus_tag="VFR42_10715"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEU0369675.1"
                     /translation="MSLFVGITLLNFTTNKDNLDFSEFYGAAQMVRQGHGKDLYDLDL
                     QAEFQSRVAAVHAVYLRPPFETLLFVPLTYVSYRTAYTLWTLANVAFLALAAFLIESR
                     TGIALAVAQ"
BASE COUNT          625 a          860 c          816 g          616 t
ORIGIN      
        1 aagccgtcct tggcaagctg aagcccggaa ccaacgtggt cttcaaggta cttcgccggc
       61 aagactcgga tcgcattttg accgtgtttc tgcccggtgt ggtcccggcg ggcaacgctc
      121 aataaaccag taaaggtttg atggacaacc accgaaaaaa ggcgtagaag agaaagcccg
      181 agaaaacacg atgattcgaa accttcgtcc ggcactcctt atcctgctgc ttagcgcggt
      241 cgggtggggt gtgccggcag gttttgcgca atcaaagagc gcagcgaaga aaaagacgcc
      301 gacggcatcg aagggaactt ctactcccag cgccccttcg acgtccgcga ccaagagttc
      361 tgcaaaggct tcttcctcca aaacgaccgc agccaaggct tcaccggccg gccagaaaac
      421 cgctggcagt ggtgccaccg taaaatcttc ggtccgcagt ggcagttcgc gcagcgcagg
      481 gaagtccagc cgccgttccc gccggcaacc tggacaaaag gccccaacca gcgatcgcgt
      541 tagcgaaatt caggcagctc tggcgaaaga tggatcattc tcgggaatgc caaacggaaa
      601 gtgggatgac gaaacgaccc aggccatgcg cagatttcag gcggcccacg ggctcaatgc
      661 aagcggcaaa ctggatgcgc tcagcctcca gaagcttggc ttgggctctc agactgccgg
      721 cgtagcggcg cccacccctc cgccaggcgc ggtctcccgg cttacttctt cagtagtctc
      781 accttccgct tcaaccgaaa ccgcaggtcg gcagtagctc tcgatttgaa agcaagcgtg
      841 ttgtgatctt aagcgagcgc cacttcagtt gagctccgct tgcccatcgc tttcgctttt
      901 cgcttccagg ctcaattcca ctgtggcgtc gctcggagca ggcgcaccca gcaagacatc
      961 aggaacagat ttagcagact gctgcgcggt tgcctcgcgt atcgctttca gcaacaactc
     1021 cggcgaggcg cttttggaaa ccacgcaccg cgcgcccgct tttttcgctt ccaattcaac
     1081 ctgcggcgat tcgtgcaaag agtagatgat gatcggaagt gtaggttcgc gcttcacaat
     1141 ttcggcggcg gcgtggaaac catccagcac tggcattgcg atgtccagga tcagaagatc
     1201 gggcttcagc tgggtggcaa gctggatcgc ctgccacccg tcgctggcct ctccacatac
     1261 ctgcaaatcc tggtgatcgg cgaagagggc ttttagggac acacggacca gttcgctgtc
     1321 gtctgcgatc aggattctgg ccattctccg ccttgtctgg acatcgaatc aaatcctgct
     1381 tgtgtagggc atcgaacact tccaaattaa tgggaaacgg tatagcgcag cgcgcggcag
     1441 gtgaatcagg tgaatgcggt agccttcaga attgccgaga agataacgaa attagctcct
     1501 atttcgccaa agtcctgcct ggctggtcga tcaaggagta aaaaatacca agtttggctg
     1561 atcgcggctt ctgttcgccc accgtataac ggtctggaca taattcagcg agaaccttat
     1621 ctgccaaagg atctacatga aaaaggacaa tcgtaatctt gttggggctt tcgttcgagc
     1681 gggagagcgg accgatagct cgggcaatcc agtccgcaac ggcattctgc ggacaatctc
     1741 agatgctgag tttcgcatgg ttcgtccaca cctggcgctg gtagctatgc cgaaccattc
     1801 cgttctgcaa gaaccgagag ctgccatcaa tttcgcctat ttcccaaacg acgggctggc
     1861 ctccatcgtg gtggcgatga agggtgagcg cgatgtggaa gctggcgttg tcggctacga
     1921 aggcgccgtg ggtactgcgc tcgccgtcgg gctcaaaaca acgcccctgc gtgtcgtcat
     1981 gcagatcgaa gggaaggcct tgcggatccc tgctggcact cttgtttcgc ttcttcgccg
     2041 cacgcccgag ctgcaaatgc gtttaagtcg ctatgccgtt ttgcagggca tgcaagttgc
     2101 ccagaccgcg gcgtgcaatc gcctgcatca cgtgagtcgg cggctggcgc gctggcttct
     2161 gatggcacaa gaccgggtcg attccggcac tctggaaatc acccacgact tcctcgcgac
     2221 catgctcggt accgaccgcc ccagcgttac cctcgcggct cggagcctgc aagcgaaaaa
     2281 agccattcgc taccgtcgcg ccagcgtaga catcgtcaat cgcaagcgcc tggagtcatt
     2341 aacgtgccag tgctatggcg cggtccaaca actgaatgga cgcttgggct tggactgagt
     2401 tccaagatcg attcgcccgg cgggcccggg gcaagaactt cgcccgcaac gcccgcaaca
     2461 gttccggttt acgcgagact ccacctggtg ctaatgttcc cgcttccgca ggcaaatcgg
     2521 cagcgtgaag agagggcatc atcaaggatc ggtggattcg atctctggcg gtcctgttcg
     2581 ttgtgagtct tttcgtcgga attacgcttc tcaatttcac caccaacaaa gacaaccttg
     2641 atttttccga gttttatggg gccgcccaga tggttcgcca agggcacggc aaagacttgt
     2701 atgacctcga ccttcaggcg gaattccagt ccagagtggc cgccgtccat gcggtttacc
     2761 ttcgcccgcc attcgaaacc ttgctgtttg ttccgctcac ctacgtgagt tatcgcaccg
     2821 cctacacact gtggacgctg gcgaacgtgg cattcctggc gttggcagct ttcctgatcg
     2881 aatcgcgcac gggaatcgcc ttggcggtcg ctcagta
//
LOCUS       DASTPM010000609         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_598694, whole genome shotgun sequence.
ACCESSION   DASTPM010000609 DASTPM010000000
VERSION     DASTPM010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598694"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="VFR42_10720"
     CDS             <1..2070
                     /locus_tag="VFR42_10720"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEU0369676.1"
                     /translation="VLWGFTSAAEEGEHVLVDATDFFLRDAHGVTNTLKKTKQGTYKL
                     DSSRCAMYLPNTKNFQLNTEVEATLTFTGDEPGRWVRDVTPDPSAITVREHHSLVQLP
                     PAGYKPRVYDPRSSFFAISYRDYATPMSEPIVKRFAARHRLAKKEPTASVSEPVEPIV
                     YYLDRGVPEPIRSALLEGARWWNQAFEAAGYKNAFRVELLPEGADMMDLRYNVIQWVH
                     RATRGWSYGAGVIDPRTGEIIKGHVTLGSLRVRQDYLIAEGLLAPYEKGKPVSTKMQE
                     MALARLRQLAAHEVGHTLGLEHNYSASTVNRSSVMDYPPPVAKLTTDGSIDLSDAYAA
                     GVGEWDKVSIKWGYQDFPVGTDESAALNKTLLDGYGRGLRFLTDQDARPAGSSSSVAH
                     LWDSGTNAVDELNRLMQVRAAAVKRFGENNIREGAPMATIEDSLVPVYMLHRYQVEAA
                     SKFVGGMDYTFALRGDGEMRTKIVAPTEQRRALAAVLATLKPPALALPEALLETIPPR
                     PPEYESGREHFKIHTSPAFDALAPAEAAAQHTLQFLFNTERAARLVEFHARDVANPGL
                     QEVLDAIVAATWKAPHPGGYYGEIARVVDDVVLYDLLGLTTNEQAATQVRAVAWFEAD
                     QLKAWLNAAKASATDPAQKAHLAHGAAQVEQFQKDPKKLNLTPPSEPPDGPPIGDDED
                     FVGWPPN"
     gene            2156..3070
                     /locus_tag="VFR42_10725"
     CDS             2156..3070
                     /locus_tag="VFR42_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369677.1"
                     /translation="MVSGTLLSVVGITVWAAVLVTAAPRVISHRYSEHSVEVSTARDE
                     WPAANCNDLHIEFDGRDAVMQSEEKTITRAEASTLRVQAESNGGVQVQGWEQNNYSVT
                     LCKAARPGGDAENLLSQIKLTFANGELGISGPNSRHQWSAHLLIKAPRGAAMDLNVNN
                     GPMGLFEVEGNLKVHAVNGPITVEKCKGVLNLTAENGPISLEENSGKLNVRTQNGPIT
                     ISLEGKNWNGEGLEAHATNGPVTLRVPSGYQSGVVLESDGHGPFSCAASVCNEGRKTW
                     DENSKRVEFGSGPAVVRVSTVNGPVSVH"
     gene            3359..>4140
                     /locus_tag="VFR42_10730"
     CDS             3359..>4140
                     /locus_tag="VFR42_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0369678.1"
                     /translation="MFLEERAAAAMSATDAERIAADLYGITAVVTVLPGEYDCNFHLR
                     ASGGREFVLKCMHPAREKSFIEMQCAALEHLSKTAPHLPLPRVQRNSQGQLLAEIADG
                     ADQKRVVWMLSFLPGATLEKTNPHSPEILEGLGKFLGELDSALQNFAHPGVHRELKWD
                     SSRAAWIGDQLHHLEESSRRALVEYFISLYQEEVEPRRDELRQSAIYGDANDHNVLVS
                     PARPQPRKVAGVIDFGDMHYGWTVSEAAIAAAYAILGKENPL"
BASE COUNT          912 a         1109 c         1335 g          784 t
ORIGIN      
        1 gtgctgtggg ggtttacctc ggccgcggag gaaggcgagc acgtgctggt ggacgcgact
       61 gacttttttc tgcgcgacgc gcatggcgtg accaacaccc tcaagaaaac aaaacaggga
      121 acatacaagc tagactcttc ccgctgcgcg atgtacctac cgaataccaa gaatttccag
      181 ctgaacacgg aagtggaagc gacactgacg tttaccggag acgagccggg acgctgggtg
      241 cgcgatgtca cgcccgatcc ctcggcgatc accgtgcgag aacaccattc cttggtccag
      301 ttgccgccgg caggttacaa gccgagagtt tacgatccgc ggtcgagctt ctttgcaatt
      361 tcatacaggg actatgccac tcctatgagc gaaccgatcg tgaagagatt cgcagcacgg
      421 catcggctag cgaaaaaaga accgacggca tcggtgagcg aaccggttga gccgattgtg
      481 tactacctgg accgcggcgt gccggaaccg attcgttcgg cgctgctgga aggcgcgcgc
      541 tggtggaatc aggcgttcga agctgcgggc tacaagaatg cgtttcgtgt ggaactgctg
      601 ccggaaggcg ccgacatgat ggacctacgc tacaacgtga ttcagtgggt gcatcgggcg
      661 acgcgcggat ggtcctacgg cgcgggggtg atcgatccgc gcacaggaga aatcatcaag
      721 ggtcacgtga cgctggggtc gctgcgcgtg cggcaggatt atttgatcgc ggaaggattg
      781 ctggctcctt atgaaaaggg aaagccggtc tctacgaaaa tgcaggagat ggcgctggct
      841 cgcttgcggc aattggcagc gcatgaagtg ggacatacct tgggattgga gcacaactat
      901 tccgcgagca ccgtgaaccg atcgtcggtg atggattatc cgccgccggt ggcgaagctg
      961 accacggatg gttcgattga cttgagtgac gcatacgcgg ccggagtcgg tgagtgggat
     1021 aaagtttcga ttaagtgggg ctaccaggac tttccggtcg gaacggatga atcggcggcg
     1081 ctgaacaaga ctttgctgga cgggtacggg agaggcttgc gctttttaac cgaccaggat
     1141 gcccgtcccg cgggaagctc cagcagcgtg gcacatttgt gggattccgg cacaaacgcg
     1201 gtggacgaac tgaaccgttt gatgcaggtg cgtgccgcgg cggtaaagcg gtttggcgaa
     1261 aacaacattc gcgaaggtgc gccgatggcg acgatcgagg attcactggt gccggtgtat
     1321 atgttgcatc gctaccaggt ggaagcggcg tcgaaattcg tggggggaat ggactacacg
     1381 ttcgcattgc gcggggacgg cgaaatgcgg acgaaaattg tggcacctac ggagcagcgt
     1441 cgcgcactgg cagcggtgtt ggccacgctg aagccgcccg cgctggcatt gccggaagcg
     1501 ctgctcgaaa caattccgcc acgtccaccg gaatacgaaa gcggccgcga gcacttcaag
     1561 atccacacga gtccggcgtt tgacgcgctg gcgccagcgg aagcggcggc gcagcatacg
     1621 ctgcaatttc tgtttaacac cgagcgcgcc gcgcggcttg tggaattcca tgcacgcgat
     1681 gtagcgaatc cggggctgca ggaagtgctc gacgcgatcg tggccgccac ctggaaggct
     1741 ccgcacccgg ggggttacta cggtgaaatt gcccgtgtgg tggacgacgt tgtgttgtac
     1801 gacctgctcg gcttgaccac aaacgaacaa gcggcgacgc aggtacgagc cgtcgcgtgg
     1861 tttgaagctg accaactaaa agcatggctg aatgcggcaa aggcgtccgc gacggacccc
     1921 gcacagaaag ctcacctggc ccacggagcg gcgcaagtcg agcagtttca gaaagatccg
     1981 aagaaactga acctgactcc accatcggag ccgcccgacg gaccgcccat cggcgacgat
     2041 gaagatttcg tcggctggcc gccaaattag gtttgcagcg gcggctcgaa aatcttttgc
     2101 gaaacgacct gaccatcggg ccctggtatc gtctatatgg ctggaggtga ggtccatggt
     2161 ttcaggcacg cttctttccg tggtcggcat cacagtttgg gcagcagtcc tggtgactgc
     2221 cgcgccgcgg gtgatttcgc accgctacag cgaacactcc gtcgaagtct cgacggcacg
     2281 tgacgaatgg ccggcggcca actgcaacga cttgcacatt gaattcgacg gccgcgatgc
     2341 cgtcatgcag tctgaggaaa agaccatcac aagggccgaa gcttcaactc tgcgtgtaca
     2401 ggccgaatcg aacggtggcg tacaggtgca aggctgggag cagaacaact attcggtgac
     2461 cttgtgcaag gcggctcgtc ccggcggcga cgcagagaac ctgctgagcc agatcaaact
     2521 cacatttgcc aacggcgaac tcggtatcag cggacccaac tcacggcatc agtggagcgc
     2581 gcatcttctg atcaaggcgc cacgcggcgc agcgatggac ctgaacgtca acaatggccc
     2641 gatgggattg ttcgaggtcg agggaaatct gaaagttcat gctgtgaacg gcccgatcac
     2701 tgtggaaaag tgcaagggcg ttctgaatct gacggcggaa aatggtccaa tctcgcttga
     2761 ggagaacagc ggcaagctga acgtgcggac gcagaatggg ccgattacca tcagcctgga
     2821 aggaaaaaac tggaacggcg agggtctgga agctcatgcg acgaatggac cggtcacgct
     2881 gcgcgtgcct tcgggatatc agtcaggcgt ggtgctggaa tcagacggcc acgggccgtt
     2941 cagctgtgca gcgagcgttt gcaacgaagg gcgcaagacc tgggacgaga acagcaagcg
     3001 cgtggaattc ggctcgggac cggcggtggt aagagtttcc acggtgaacg ggcctgtcag
     3061 cgttcactaa aaagaactag ttcgactggg cccgttccca ggccggcggg actctgacgg
     3121 aggccaatcc cgctgaggta gcgacctgag cttttccgcg aaatcgcaat tccattcggc
     3181 gatcgtgccg tcgcaatcaa acagataggc gtggaaattt ccttggcgga atcctggttt
     3241 tcattctgtc ttcatagcct cgcttccatc ttagggcagg aaccgcgtcg acgcgccagg
     3301 cggctccgca ttggctgcga ttggacggta gccgccgaat gccgataaga tttcaggcat
     3361 gtttctggaa gaacgcgcag cggcggcgat gagcgcaacg gatgccgagc gtattgcggc
     3421 tgacttgtac ggcatcacgg ctgttgtgac ggtattgcca ggcgaatacg actgcaactt
     3481 tcatttgcgt gccagtggcg gtcgggaatt tgttttgaag tgcatgcacc cggcgcgcga
     3541 gaaatcattt attgagatgc aatgcgccgc gctggagcac ctttcaaaaa ccgctccgca
     3601 cctgccactg ccgcgcgtgc agcggaacag ccaaggccag ctgttagccg agattgccga
     3661 cggcgcggac cagaaacgcg tggtgtggat gctgagtttc ctgccgggag ccacgctgga
     3721 gaagaccaat cctcattcgc cagagatctt ggaaggtttg ggaaagtttc ttggcgaact
     3781 agactccgcg ttacagaact ttgcccatcc gggagttcac cgagagctga aatgggattc
     3841 gtcgcgcgcc gcatggattg gcgatcaatt gcatcactta gaggaatcgt cgcgtcgcgc
     3901 gctggtggaa tattttattt cgctttatca agaggaggtc gaaccgcgcc gcgacgaact
     3961 gcgccagagc gcgatctacg gcgacgcgaa tgaccacaac gttttggtca gccctgcgcg
     4021 gcctcagccg cgaaaggttg ccggtgtgat cgactttggc gacatgcatt acggctggac
     4081 ggtcagcgag gcggctatcg cggcggcata cgccattctg ggtaaagaaa atccgctgag
//
LOCUS       DASTPM010000610         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_281847, whole genome shotgun sequence.
ACCESSION   DASTPM010000610 DASTPM010000000
VERSION     DASTPM010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281847"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFR42_10735"
     CDS             <1..327
                     /locus_tag="VFR42_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0369679.1"
                     /translation="INGRRQLSCLAFAALHEGDEITTIEGIGQPEKMHPMQAAFVECD
                     AYQCGYCTSGQIMSAVALLKEPCGPEDAEVKEFMSGNICRCGAYQNIVAAIQQVRQGT
                     KGQKVG"
     gene            324..1328
                     /locus_tag="VFR42_10740"
     CDS             324..1328
                     /locus_tag="VFR42_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0369680.1"
                     /translation="MKTFHLTRPKDSAQAIATVAKSKTAQQGADIRFIAGGTTLIDLM
                     KLNVEQPQNLVEVNRLTLDKIEATPDGGLTIGATVRNSDLAWHPLVRENYRVLSEAIL
                     SGATVQIRNMATTAGNLLQRTRCMYFRDPAMPCNKREPGSGCAAITGVNRGLAILGTS
                     EHCIATNPSDMNVAMAALEATIHVRGPKGERAIPIADFHVLPGNTPQRETVLEPGDLI
                     THVTLPPPAPGNRSLYLKLRDRMSYEFALASAAVVITAANGKITRARIALGGVGTKPW
                     RSTDAESALTNQPTTKDVFQKAAEAALRGAKPQSQNGFKVELAKRCLVHALKLATQPA
                     "
     gene            1330..>2110
                     /locus_tag="VFR42_10745"
     CDS             1330..>2110
                     /locus_tag="VFR42_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0369681.1"
                     /translation="MPQETTASPIGRDTPRIDGPLKVTGVAQYASDFHFPGLLYGVPV
                     EATIANGKITKLDTAAAEKMPGVRAILYRQNIGKIFRSTLGKGVNGICIERRPPFDDD
                     IIRYYGQYVALAVADTFESAKAAADAVVVGYAKEKPNVEDKLTADDDPDEVMTSYGPE
                     KRLQSERGDAAGAFASAPVKLDQTYVTPAETHVPIELQGTTAMWDGDHLTIYEESQAV
                     FNMRTVLAQMFGLPEENVRVITKFVGSGFGSKLWPWTHCPLA"
BASE COUNT          478 a          632 c          626 g          374 t
ORIGIN      
        1 atcaatggtc ggcgccagct ttcgtgtctc gccttcgctg ctctgcacga gggcgatgag
       61 atcacaacca tcgaaggcat cggtcagccg gaaaaaatgc atccgatgca agcggcgttt
      121 gtggaatgcg atgcctatca gtgcggctat tgcacctccg gccagatcat gtcagcggtt
      181 gcgcttttga aggaaccgtg cgggccggaa gacgcggagg tgaaggaatt catgagcggt
      241 aacatctgcc ggtgcggcgc gtatcagaac atcgtcgctg caatccaaca ggtgcggcaa
      301 gggaccaagg ggcagaaagt gggatgaaga cttttcacct cacgcggccg aaagacagcg
      361 cgcaagcgat cgcgacggtg gcgaagtcga aaacggcgca acagggagca gatatccgtt
      421 tcattgcagg tggaacgacg ctgatcgact tgatgaagct caacgtcgag caaccgcaaa
      481 atttggtcga agtgaaccgg ttgacgctcg acaaaatcga ggcgacgccc gacggcggcc
      541 taacgattgg tgccacggtg cgcaattccg acctggcctg gcacccactc gtgcgcgaga
      601 attaccgcgt tctttccgaa gcgattctga gcggagcaac cgtgcagatt cgcaacatgg
      661 caacaactgc cggcaacctg ctgcaacgga cgcgctgcat gtatttccgc gatcctgcaa
      721 tgccttgcaa caagcgtgag cccggttccg gttgcgccgc gatcaccggg gtgaatcgcg
      781 gcctggccat tctcggcacg agtgaacatt gcatcgcaac caatccctcc gacatgaacg
      841 tggccatggc cgcgcttgag gcgacgattc acgtgcgtgg tccgaagggc gagcgtgcga
      901 ttccgattgc agactttcac gtcttgcccg gcaacacacc gcaacgcgag accgtgctcg
      961 agccgggcga cttgatcacg catgtcaccc ttccgccgcc tgctccggga aatcgctcgc
     1021 tgtatctaaa actgcgtgac cgcatgtcct acgaattcgc gctagcttcc gcggcagtcg
     1081 tgatcactgc ggccaatgga aaaattacgc gtgcgcgcat tgccttgggc ggtgtcggca
     1141 ccaaaccgtg gcgatcgacc gacgcggaaa gtgccctgac aaatcagccg acaacaaaag
     1201 acgtttttca aaaagcggcc gaagccgcgc tgcgcggtgc aaaaccccaa agccagaacg
     1261 gattcaaggt ggagttggcc aagcgctgcc ttgtccacgc gctcaagctt gcaacgcagc
     1321 ccgcctaaca tgcctcagga aactacagca tcgccgatcg gtcgcgacac accgcgcatc
     1381 gatggtcctc tcaaggtgac tggcgtggcg cagtacgcct ccgactttca ctttcccgga
     1441 ttgctgtacg gcgttccggt cgaggccacg attgccaatg gaaagatcac gaagctcgac
     1501 acagccgccg cagagaagat gcccggcgtc cgcgcgattc tttatcgtca aaacatcggg
     1561 aagattttcc gttcgactct cgggaaaggc gtgaacggca tctgcatcga acggcggccg
     1621 ccgttcgatg atgatatcat tcgctactac ggccaatacg ttgcgctcgc ggtggcggac
     1681 actttcgaga gcgccaaagc cgcggcggac gcggttgtcg ttggctacgc gaaagagaaa
     1741 ccgaacgtcg aagacaagct caccgccgat gatgatcccg acgaagtgat gaccagctac
     1801 ggaccggaga agcgcctgca aagcgagcgc ggcgatgcgg ccggagcctt tgcgagcgcg
     1861 ccggtcaagc tcgaccagac ttacgtgacg ccggccgaga cgcacgttcc catcgagctg
     1921 cagggaacaa ccgccatgtg ggacggcgat cacctgacca tctacgagga atcgcaagcc
     1981 gtcttcaaca tgcgcaccgt actcgcgcaa atgttcggtc tgccggaaga aaacgtgcgc
     2041 gtcatcacaa agttcgtcgg ctccggcttt ggcagcaaac tttggccatg gacgcactgc
     2101 ccgctggcag
//
LOCUS       DASTPM010000611         3428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_310174, whole genome shotgun sequence.
ACCESSION   DASTPM010000611 DASTPM010000000
VERSION     DASTPM010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310174"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..713)
                     /locus_tag="VFR42_10750"
     CDS             complement(144..713)
                     /locus_tag="VFR42_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369682.1"
                     /translation="MKLRIKEVLPGPRVQKSAGLGLLSWRWLAVAAALLLATFVGWRQ
                     LSTGNQRTYGEAAVVAVVDAHLRSLQPGHLVDVQSSDQHTVKPWFDGKLDFAPPVRDF
                     SSADFPLLGGRLDVIEGRTVAALVYGRRKHIISVFVEEAGSDSSWKGSGEAQGYHWTA
                     WNKDGFAFCAVSDVSPIDLDGLKHLFLQE"
     gene            complement(889..1446)
                     /locus_tag="VFR42_10755"
     CDS             complement(889..1446)
                     /locus_tag="VFR42_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0369683.1"
                     /translation="MPPDAPTDLLNQDDRSRFEAIVMPHLDAAYNLARWLLGGRSEAE
                     DVTQDAMLRAYRFFNTFHSGDARAWLLQIVRNCCYTWLQKNRNWVDVDELDDTSVPRE
                     KDTPETLAISVNDRERLTRALEQLSPHFREILVLRELEGCSYKEIAAITSRPMGTIMS
                     ALARARQQLRVLLTEPAPKEARLEL"
     gene            1618..2583
                     /locus_tag="VFR42_10760"
     CDS             1618..2583
                     /locus_tag="VFR42_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0369684.1"
                     /translation="MTRKKDEIQFDHNHDGIDRRGFLKCMAWAGTGAFCVMQGGVLKS
                     YSLSRIAGVDPSTMKGELSFAQVSDSHMGFSKLANPDVAGTLKAAVDKINALGTSPEF
                     LLHTGDISHLARPEEFDNVEQILRSAVPKDVFYVPGEHDVVGDDGKEYLRRYGKNSKG
                     AGWYSFDKKGVHFVGLSNVMNLKAGGLGSLGPEQLEWLEDDVKPLSASTPIVVFAHIP
                     LWSVYPQWGWGTEDSAQALGYLKKFGSVTVLNGHIHQTMQKVEGNVTFHTAASTAFPQ
                     PEPGKADSPGPMKVPADQLRSLLGITDVNFVQGRDALAIVDSKLG"
     gene            2623..2982
                     /locus_tag="VFR42_10765"
     CDS             2623..2982
                     /locus_tag="VFR42_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEU0369685.1"
                     /translation="MNRRKRYLAGAVLLVGLVGALLGTTRSASGSPAQAAAGDEVKID
                     NFTFAPGEITVVKGTTVKWVNRDDIPHTVVSDDKSTFKSKPLDTDDKFSYTFTKEGTY
                     NYFCSIHPKMTAKVIVK"
     gene            2979..>3428
                     /locus_tag="VFR42_10770"
     CDS             2979..>3428
                     /locus_tag="VFR42_10770"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU0369686.1"
                     /translation="MIGASTNPFDWRTVLLARHAQHVVLIHFPIALYLAGVVLDLAGS
                     WWDKEKFGETAYYNFTLAAIFALPAVLTGLVAWQWQLEGQRLKGTLLEHLLAGLTAAV
                     VICLTWTLHFLVRRKKSNLPRARFLLEAAGTLAIVVAGHLGGFLSGVN"
BASE COUNT          786 a          945 c          994 g          703 t
ORIGIN      
        1 tcctgtactg acgcaggatt tcgtcattgt aggttatggt agtactccac gattgactgt
       61 cgcagatcta ctgaggtttc ggcttttttg aactcataat ccgttggtcc caggttcgaa
      121 tcctggcggg cccaccaacc ctactattct tgaaggaaca ggtgctttag cccgtcgagg
      181 tcgatcggag aaacgtcgga cacggcacaa aacgcgaatc catctttgtt ccacgcggtc
      241 cagtgatagc cttgcgcttc gcctgagcct ttccacgaac tgtcggaccc tgcctcctcc
      301 acaaagacgc tgatgatgtg cttgcgacgg ccataaacca gcgctgcgac cgtgcgccct
      361 tcaatcacat ccaggcgtcc gccgagcaag gggaagtcgg cggaagagaa atcgcgaaca
      421 ggcggcgcga aatcgagctt gccgtcgaac catggtttga cggtgtgctg atcggaggac
      481 tgcacgtcca ccaaatggcc aggctgcaga gagcggagat gcgcatcgac cacggcgaca
      541 actgcggctt cgccgtaagt tcgctgattc ccggtgctca gctgccgcca gccgacgaag
      601 gtggcaagca acagtgccgc cgcaactgca agccagcgcc acgaaagcaa gcccaagccg
      661 gcagattttt gtacgcgagg gccagggagg acttctttta tgcgcagttt caagctagct
      721 ggagccctcg agtaaagcct ggattgctgt aaaccctgac gaagtgcttc ttcctcttct
      781 aaggcacccg cacaaacagg acagtttcgc agatgttggt tgaaatcggc cgaaccggcg
      841 gcatcgagtt cgccatccag ataggcgtgc aaccgagctt gcgcgagatc acaactcaag
      901 gcgagcctcc ttcggagccg gctcggtgag cagaacgcga agctgctgcc gcgcacgggc
      961 cagcgcggac atgatcgtgc ccatcggccg cgaggtaatt gctgcgatct ccttgtagga
     1021 gcaaccttcc aattcgcgca acacgagaat ttcgcggaag tgaggcgaaa gctgctcgag
     1081 ggcgcgagta agccgctcgc ggtcgttgac ggaaatcgcg agagtctctg gtgtgtcctt
     1141 ctcgcgggga accgaagtat cgtccagttc atccacgtcc acccaattgc gattcttctg
     1201 cagccaggtg taacagcagt tgcgcacaat ttgtagcagc caggcgcgcg catcgccgga
     1261 atgaaatgtg ttgaaaaaac ggtatgcgcg caacatagcg tcctgcgtca cgtcctctgc
     1321 ctcgcttcgg ccacccagaa gccatcgcgc caggttgtaa gcggcgtcca gatgcggcat
     1381 cacgattgcc tcaaagcggc tgcgatcatc ctgattcaac aagtccgtgg gtgcgtccgg
     1441 cggcacgtca gcctctttca ggctctacga gagacaaccg agggatgttc gattttattc
     1501 ccgcccaatc gaatttatct aacaagaaaa attagtccgc caacggaata aaggcgcgac
     1561 cggacgagtc ttgcatagtg aaacgcatca agccagacga actgcaagga gtttaccgtg
     1621 acgcgaaaaa aggacgaaat tcagtttgac cacaaccacg atggcatcga ccggcgtggc
     1681 ttcttgaagt gtatggcatg ggcgggtacc ggtgcgttct gcgtgatgca gggaggagtg
     1741 ctgaaatctt acagcttgag ccgaatcgcc ggcgtcgatc cttcaaccat gaaaggcgag
     1801 ctgagtttcg cgcaagtcag cgacagccac atgggcttta gcaagctagc caatcccgat
     1861 gtggccggca ctctaaaggc ggccgtcgac aagatcaatg cgctcggcac gtcgccggaa
     1921 tttctgctgc acactggcga catcagccat ctcgccaggc cggaagaatt cgacaacgtt
     1981 gagcaaatcc tccgaagcgc cgtgcccaaa gacgttttct atgttccggg cgaacacgac
     2041 gtggttggcg atgacgggaa agagtatctc aggcggtacg gaaaaaattc caaaggcgcc
     2101 ggttggtaca gctttgacaa gaaaggcgtg cacttcgtcg ggctttcaaa cgtgatgaac
     2161 ctgaaggcgg gcggcttggg atcgttgggc cccgaacaat tggagtggct ggaggacgac
     2221 gtcaagcctc tttcagccag tacgccgatc gtcgtgttcg cgcatattcc gctctggtcc
     2281 gtgtacccgc agtggggctg gggcacggag gacagcgcgc aggctctcgg ctacttaaag
     2341 aaattcggtt cggtgaccgt acttaacggc cacatccacc agacaatgca aaaagttgag
     2401 ggcaacgtga cgtttcacac tgcggcttcg acggcatttc ctcagcccga acccggcaaa
     2461 gccgattcgc ccggcccgat gaaagtgcct gcggaccagc tacgcagctt gctgggaatc
     2521 accgacgtga attttgtgca aggcagggat gccctggcga ttgtggattc gaagttgggt
     2581 taaatcggtc tgtcgaaaga aattcaaaca aggagaaaac aaatgaatcg caggaaaaga
     2641 tatctggctg gcgcagtgtt gctcgtcgga ctcgtaggag cgctcctcgg gacgacacgc
     2701 tccgcgagcg ggagccccgc ccaggcagcg gcgggtgacg aagtcaaaat cgataacttc
     2761 accttcgctc cgggtgaaat caccgtggtt aagggaacga ccgttaagtg ggtgaatcgc
     2821 gacgacattc cccacaccgt cgtaagcgac gacaagagca ccttcaaatc caagccattg
     2881 gataccgacg ataagttttc ctacaccttc accaaagaag ggacctacaa ctacttctgc
     2941 tcgattcacc ccaaaatgac ggcgaaggtg atcgtcaagt gatcggcgca tccacaaatc
     3001 cgtttgattg gcggaccgtg ttgctggcgc ggcacgcaca gcacgtggtg cttattcact
     3061 ttcccatcgc tctgtatctg gcaggtgtgg ttttggatct ggccggatca tggtgggaca
     3121 aggagaaatt tggagagact gcctattaca atttcaccct cgctgcgatt ttcgctctgc
     3181 cggcagtact aaccggtctg gtggcctggc agtggcagct ggaaggccag aggttgaagg
     3241 gcactttgtt ggaacatctg ttggcagggc tcacggcggc cgtagtaatt tgtctcactt
     3301 ggacgttgca ctttctcgtg cgccgtaaga agagcaattt gccgcgggcc cgcttcctgc
     3361 tggaggcagc cggaacctta gcgatagtcg tcgctggcca cttgggtgga tttcttagcg
     3421 gcgtaaac
//
LOCUS       DASTPM010000612         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_858172, whole genome shotgun sequence.
ACCESSION   DASTPM010000612 DASTPM010000000
VERSION     DASTPM010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858172"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFR42_10775"
     CDS             complement(<1..205)
                     /locus_tag="VFR42_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369687.1"
                     /translation="MKFVISLFLCAAVTCEAQTAPLPQLRQNGAVKQFFVDDKPFIML
                     AGELHNSSASSIEYMLPVWDKLAA"
     gene            complement(612..1067)
                     /locus_tag="VFR42_10780"
     CDS             complement(612..1067)
                     /locus_tag="VFR42_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0369688.1"
                     /translation="MLYSYIVKKKIRQTFDDVNNHRWDEAVAAVAPQVHHRVSGIHAL
                     AGERNDKEALRCWFQRLGRVQPNLRLRINNIWVNGWPGNTTVFVQWDGTATLLNGDAY
                     VNRGLHVFTLRWGRVYRLEEFQDSEAAAHALSTQAAAGLKEAVADPIVS"
     gene            complement(1186..2052)
                     /locus_tag="VFR42_10785"
     CDS             complement(1186..2052)
                     /locus_tag="VFR42_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0369689.1"
                     /translation="MEKIFGEDQQQDSPGRPNDKHAADNIYERIPMKLTNRTILITGG
                     SAGIGLAFALKFLELGNQVIVTGRRQSVLDELRANYPKLHTIQSDVADSAQIAALARR
                     VKAEFPKLDVLMNNAGIMLHKNLKVPTADLDGLMAELNINAGGVVRTTSAFIDILIAN
                     KGTVINVSSALAFVPLPSAPIYCATKAAVHAYTQSLRFQLEETGVEVIELMPPAVKTD
                     LSADIAEGEGITLITTDELVKQSFASLKAGALEVRPGQAKLLALMRRLAPDFINRQLW
                     KASKKLVPVGVA"
     gene            complement(2052..>2297)
                     /locus_tag="VFR42_10790"
     CDS             complement(2052..>2297)
                     /locus_tag="VFR42_10790"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0369690.1"
                     /translation="VVEATVQRSRPVILTALAAVLAFIPLTFSVFWGSMAYTLIGGTA
                     VGTVLTLIFLPALYSIWFPVKPSAGPDDEAHAVPVHA"
BASE COUNT          519 a          628 c          647 g          503 t
ORIGIN      
        1 gagcggcgag cttgtcccat accggcagca tatactcgat gctcgaagca ctcgaattgt
       61 gcaattcacc tgcgagcatg atgaatggct tgtcgtcgac gaagaactgt ttgaccgcgc
      121 cattctggcg gagctgcggc aagggagccg tttgcgcttc gcaggtcaca gcggcgcata
      181 aaaagagaga aattacgaac ttcatgtagt ccataagttt cgataaccct cattcacgcc
      241 gccaataaca gcgacggccg cgtcggctat tctaagcgac caaagctcat tcccatagtg
      301 atcgagaatg actgccttca tgacctatcc catcctcgcg gcaccagctt cttgcaaaga
      361 ccctttcact aaattccgag aaccccagcg cgatggcctc ggcgaagagc caaggtgagg
      421 cttcgggggg attgggcgct tttacggccg cgccaagtcg aggagtcgga tcagtagcga
      481 aagaggctcc agaagcccag tttctgtagc gtcgaagcgg aaatgcctgt tttcctgacc
      541 tcgcgaaatt tagagggacc acaagcctga actcaaagaa agcgacagcc aggcaccagg
      601 gatttgcttt gctagctcac aattgggtca gcgacagctt ctttgagtcc agctgccgcc
      661 tgtgtggaga gagcatgggc cgctgcttcg gaatcttgaa attcctcgag tcgatagact
      721 ctgccccacc gcagagtgaa cacgtggaga ccacggttga cgtacgcatc accgttaagc
      781 aacgttgcag tgccgtccca ttggacaaac acggtggtgt tacccggcca accatttacc
      841 cagatgttgt tgatcctgag acggagattg ggctggacac ggccgaggcg ttgaaaccag
      901 cagcgcaagg cttctttgtc gttgcgctca ccagcaagcg cgtggattcc cgaaacgcga
      961 tgatgaacct gcggcgcgac cgctgccact gcctcatccc agcgatggtt gttgacgtca
     1021 tcgaaggtct gccggatttt tttcttaaca atgtaactgt ataacatttt tagtccctgt
     1081 aacttatgtg aaaagatatc ttacggactg tccagcaaca gggtgaccag tccgcaggcc
     1141 acgccgcgcg ctgtcaccca agtgcaccgc gatggtttcc cggccttacg ccacgccaac
     1201 cggaaccaat ttcttagagg ctttccagag ctgccggttg atgaaatcgg gtgcgaggcg
     1261 gcgcatcagc gcgagtagct tcgcctgtcc cggccgaacc tcgagtgctc ctgccttgag
     1321 ggacgcaaag gattgcttca ccaattcgtc tgtcgtaatt aaagtgatgc cttcgccctc
     1381 cgcaatgtct gccgacaagt cggtcttcac tgccggcggc ataagctcga tcacctcgac
     1441 accggtttct tccagctgga accgcagcga ctgagtgtat gcgtgaaccg ccgccttagt
     1501 cgcacagtag attggcgccg acggcaaggg cacgaaagca agtgcggagg atacgttgat
     1561 tactgtgcct ttgttggcta tgagaatgtc gatgaatgca gaagtcgtgc ggacgacgcc
     1621 gcccgcgttg atgttcagct cagccatcaa tccgtcgagg tcagccgtcg ggaccttgag
     1681 gtttttgtgc agcatgatgc ccgcgttatt catcaagacg tcgagctttg gaaattcagc
     1741 cttcacgcgc cttgcaaggg cggcgatctg cgcggaatct gcgacgtcgc tctggatggt
     1801 gtggagcttc gggtagtttg cccttagctc atcgagaacc gactggcggc gacccgtcac
     1861 gatgacctgg ttgccgagtt cgagaaactt tagggcaaaa gcgaggccaa tacctgcaga
     1921 gcctccggtg atcaagatgg tgcgattggt gagtttcata ggaatccttt cgtaaatatt
     1981 gtctgcagca tgtttgtcat ttgggcggcc cggagaatct tgctgctggt cttctccgaa
     2041 gattttttcc actaggcatg caccggtact gcatgcgcct catcgtccgg gccggctgac
     2101 ggttttaccg ggaaccagat ggaatagagt gcgggcagga agataagggt caacaccgta
     2161 cccacggcag tgcctccaat caaggtgtat gccatcgatc cccagaaaac cgagaaggtc
     2221 aaagggatga aagccaagac agcggcaagg gccgtaagaa ttacgggacg tgatcgctgt
     2281 acggtcgcct ccacgac
//
LOCUS       DASTPM010000613         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_108540, whole genome shotgun sequence.
ACCESSION   DASTPM010000613 DASTPM010000000
VERSION     DASTPM010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108540"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VFR42_10795"
     CDS             complement(<1..802)
                     /locus_tag="VFR42_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369691.1"
                     /translation="MNKKWMTACAVVVLAGAAVAAQEATITPPESIVAEGVPKIAAAV
                     AETAGRYGAYRSAGLADWNPTKREMLIATRFGDTPQLHLVSMPGGARRQLTFFTDAVT
                     NGRFHPNGGDYLVFMKDIGGGEWYQLYRYDLQTGEVMLLTDGKARNLLGPWSTKGDEI
                     AYMSTRRTGKDTDLWVMNPADPKSDHLLAKLEGGGWEALDWSPDDKRILLKEDISINE
                     SYLWLVDMSTGEKTAFTPRDAKEKVFYGEGEFSKDGKGIYVTTDKESEF"
     gene            929..2434
                     /locus_tag="VFR42_10800"
     CDS             929..2434
                     /locus_tag="VFR42_10800"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU0369692.1"
                     /translation="MFRKILIANRGEIAVRILRACRELGIRSAAVFSEVDRNSLHVRL
                     ADEAYPVGPAPSRESYLRIDKIMNVARRAGCDAIHPGYGFLAENAALPRACADAGITF
                     IGPSAEAMETLGAKTAGRQLARRAEVPTVPGTNDPIDDPQAAQEIARSMGYPVLLKAV
                     AGGGGKGMRLVNADSEFSAAFRDASSEALNAFGNASVFLEKYLDQPRHIEIQILADAH
                     GRVVSLGERECSVQRRHQKVIEEAPSPIMTPELRKRMGDAAVRLARAGGYTNAGTVEF
                     LVDSHYNFYFLEVNTRLQVEHPVTEQVTGLDLVKLQIAVAAGHRLPFAWETITPRGHA
                     MEVRLYAEDPENNFFPSPGKILSRHAPSGPGIRVDEGVYEGWTVPMDYDPLLSKLIAW
                     GNSREETISRLRRALEEYSITGIKTNAALFRRILAEPDFLRAEIHTKWLDEMLRRPHP
                     TAHNDANGVDAAAIAAALWQATRNGSASAASAAPESAWKREGRRQQLERLP"
     gene            2431..2931
                     /locus_tag="VFR42_10805"
     CDS             2431..2931
                     /locus_tag="VFR42_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU0369693.1"
                     /translation="MKYEVLLAGKTRVVELTRQNGAWKISLDGNTLDANAVEVAPNIF
                     SVLLSGDSHQIRIAPRPDGTLTLHTGLAEYHAEVSDPRSWRGRRQGAVEAEGRQQITA
                     PMPGKVVRLLVKQGDSVEAGQGLLVVEAMKMQNEIRSPKSGKIEKLFAQEGQPVNAGE
                     VLLWVE"
     gene            complement(3008..>3768)
                     /locus_tag="VFR42_10810"
     CDS             complement(3008..>3768)
                     /locus_tag="VFR42_10810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0369694.1"
                     /translation="TEDEFQRQLKERGVTVEDLKNTVRRELSIQKLINREVVAKVTIT
                     DQDIADFYNANKPQFNVAEPQYRIAQIVVTPRKEMQVRNRKNDDATNQAEAERKVKML
                     MDRLNSGADFAQLAMDYSEDMNTAVTGGDLGYVPESALNQSDPALKKMVIGMKPGQVS
                     QPIVLKDSIRILKLVTRESAGQRGIADPQVQQSIRDTLRNRKEQLLRAAYLAVARDEA
                     GVKNYLARQVLESNGKLPAAAKSTLPGKTDAPQN"
BASE COUNT          699 a         1350 c          928 g          791 t
ORIGIN      
        1 gaaactccga ctccttgtcc gtcgtgacat agattccctt gccatccttg ctgaattcac
       61 cttctccgta gaaaaccttc tctttcgcat ctcgcggcgt aaatgccgtc ttctctcccg
      121 tgctcatgtc caccagccac aaatatgatt cattaatgga tatatcttcc ttcagcagaa
      181 ttctcttgtc gtccggcgac cagtccagcg cttcccaccc tccaccctcc aatttcgcca
      241 gcagatgatc cgacttgggg tccgccggat tcatcaccca cagatccgta tccttccccg
      301 tccgccgcgt ggacatgtag gcgatctcgt cgccctttgt ggaccacggc cccagcaaat
      361 ttcgcgcctt gccgtccgtc agcagcatca cctcaccggt ctgcagatcg tagcggtaca
      421 gctgatacca ctcccctccg ccaatgtcct tcatgaaaac aaggtaatcc ccgccgttcg
      481 gatgaaacct cccgttcgtc accgcatccg taaagaatgt cagctgtcgc cgcgccccgc
      541 ccggcatcga caccagatgc agctgtggcg tgtctccaaa ccgcgtggcg atcagcattt
      601 cgcgcttcgt cgggttccag tccgccaaac ccgcactgcg gtacgcccca tagcgtccag
      661 ccgtctctgc caccgccgcg gcaatcttcg gcactccctc cgccacaatg ctttccggcg
      721 gtgtgatcgt cgcctcctgc gctgccactg ccgctcccgc cagcaccacc accgcgcatg
      781 ccgtcatcca tttcttgttc attgtccccg tgctcctttg ccaggcgagc tttctcttat
      841 acccgaaaca attggacacg caagcgaatt gactcgtctc ccccgctcca gactgcgtgc
      901 taaacttccg ctgctttcat ttcggcccat gttccgcaaa attctcatcg ccaatcgcgg
      961 cgaaatcgcc gtgcgcatcc tgcgcgcctg tcgcgaactc ggcatccgct ccgccgccgt
     1021 tttcagtgaa gtggaccgca actccctcca cgtgcgcctt gccgacgaag cctaccccgt
     1081 aggccccgcg ccttcccgcg aaagctatct ccgcatcgac aagattatga acgtcgcccg
     1141 ccgggccggt tgcgacgcca tccaccctgg ctacggcttt cttgccgaaa acgcagcgct
     1201 cccccgcgcc tgcgccgacg ccggcatcac gtttatcggc ccctccgctg aagctatgga
     1261 aacccttggc gcaaaaaccg ctggccgcca actcgcccgc cgcgccgaag ttcccaccgt
     1321 ccccggcacc aacgatccca tcgacgaccc gcaagccgcg caggaaatcg cgcgcagtat
     1381 gggctacccg gtcctcctca aagcagttgc cggcggcggc ggtaaaggca tgcgcctggt
     1441 caacgccgac tccgaattct ccgcggcctt ccgcgacgct tcctccgaag ccctcaacgc
     1501 tttcggcaat gccagcgtct ttctcgaaaa atatctcgac cagccgcgcc acatcgaaat
     1561 ccagatcctc gccgatgccc atggccgcgt cgtttctctc ggcgaacgcg aatgctcggt
     1621 tcagcgccgc caccaaaaag tcatcgaaga agctccctcg cccatcatga cgccggagct
     1681 gcgtaagcgc atgggggacg ccgcggttcg cctcgcgcgc gccggcggct acaccaatgc
     1741 tggcacggtc gaattcctcg tcgactccca ctacaatttt tattttctgg aagtgaacac
     1801 ccgcctgcag gtcgaacatc cggtcaccga acaggtcact ggcctcgacc tcgtcaagct
     1861 gcagatcgcc gtcgccgccg gccaccgcct cccgtttgct tgggaaacca tcaccccgcg
     1921 tggccacgcc atggaagttc gtctgtacgc cgaggatccc gaaaacaatt tctttccttc
     1981 acccggaaaa attctttctc gtcatgcgcc cagcggcccc gggattcgcg tcgacgaagg
     2041 cgtctacgaa ggctggaccg tcccgatgga ctacgatccg cttctcagca aactcatcgc
     2101 ctggggcaac agtcgcgaag aaaccatttc tcgccttcgc cgcgccctcg aggaatattc
     2161 catcaccggc atcaaaacca acgccgcgct tttccgccgc atcctcgccg agccggactt
     2221 cctgcgcgcc gaaattcaca ccaagtggct cgatgaaatg ttgcgccgcc cgcatcccac
     2281 cgcgcacaat gacgccaacg gcgtggatgc ggctgccatc gcagctgcgc tctggcaagc
     2341 gacgcgcaat ggctcagcgt ccgccgcatc agccgcgccg gaatccgcct ggaagcgcga
     2401 aggccgtcgt cagcaattgg agcgcctgcc gtgaagtacg aagtcctgct cgccggaaaa
     2461 acgcgcgtag tcgaactcac ccgacaaaat ggcgcttgga aaatttctct ggatggaaac
     2521 acgctcgatg ccaacgccgt cgaagtcgcc cccaatatct tcagcgttct gttgagcggc
     2581 gactcgcatc aaatccgcat tgctccgcgt cctgacggca cgctcactct tcacactggc
     2641 cttgccgaat atcacgccga agtcagcgac ccgcgttcct ggcgcggtcg ccgacagggc
     2701 gccgtcgaag ccgaaggccg ccagcaaatc actgcaccca tgcccggcaa agtcgttcgt
     2761 ctgctcgtta agcaaggcga ctccgtcgaa gccggccagg gcctgctcgt cgtcgaagcc
     2821 atgaaaatgc agaatgaaat ccgctcgccc aaaagcggaa aaatcgaaaa actcttcgcc
     2881 caagaaggcc agcctgtcaa cgccggcgaa gtcctccttt gggtcgagta gttgtagcgg
     2941 ccgccttctg aggcgggcgc ttctgtgttg ttcaggatta caactcgctg ttgcttaggc
     3001 ggcaggtcta attctgcggc gcatcggtct tgcccggcag cgtgctcttc gccgccgcgg
     3061 gcaacttccc attcgattct agaacctgcc gcgccaggta attcttaacc cccgcttcat
     3121 cccgcgccac cgccaaataa gccgcgcgca acagctgttc cttgcgattg cgcagtgtat
     3181 cccggatcga ctgctgcacc tgcggatcgg caatccctcg ttgtcccgcc gactcccgtg
     3241 tcaccagctt caaaatccgt atgctgtctt tcagcacgat cggctggctc acctgccccg
     3301 gcttcattcc gatcaccatc ttcttcaacg ccgggtccga ctggttcagc gccgactccg
     3361 gcacataacc cagatcgccg ccggtcaccg ccgtattcat gtcctcggaa taatccatcg
     3421 ccagttgcgc aaagtccgcg ccgctgttca gccggtccat cagcattttc actttgcgct
     3481 cggcttcggc ctgattggtc gcgtcatcgt tcttgcggtt gcgcacttgc atttccttgc
     3541 gcggtgtcac tacgatctgc gcgatgcgat attgcggctc agccacgttg aactgcggct
     3601 tgttcgcgtt gtagaagtcc gcgatgtcct ggtcggtgat cgttaccttg gccaccactt
     3661 cgcggttgat cagtttttga atggaaagtt cgcgccgcac cgtgtttttc aaatcttcca
     3721 cggtcacgcc acgctctttc agctgccgtt gaaattcgtc ctcggtgt
//
LOCUS       DASTPM010000614         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_425899, whole genome shotgun sequence.
ACCESSION   DASTPM010000614 DASTPM010000000
VERSION     DASTPM010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425899"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1331
                     /locus_tag="VFR42_10815"
     CDS             267..1331
                     /locus_tag="VFR42_10815"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0369695.1"
                     /translation="MDHKDYVNRAVEASIHIGLVLLLAATCFLILRPFLPLIAWGIIV
                     AIAVYPRYRQLQLVLGGRGVLAAVLITLLLLALLTVPVVLLAGTLIDGIQTLAAHLKD
                     GTPIIPPPPAGVETWPFVGVPLKSAWSLASTNITTALRSFAPQIRAIVPGLLSASAGI
                     GLTVLQFALSIIVAGALLANSRGAAAAAHSLANRIFGDKGSEFEQLVGSTIRSVTIGI
                     LGVALIQSVLAAVGFLVAGLPGAGLWAAVFLVAALLQVGTLVLIPAVIYVFATSSATK
                     AIIFMAWCLIVALIDNIVKPLLLGRGVAVPIAVVFLGAIGGFVAMGLIGLFVGAIVLS
                     VGYKLFIAWLARTPAVLGET"
     gene            1581..2642
                     /locus_tag="VFR42_10820"
     CDS             1581..2642
                     /locus_tag="VFR42_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU0369696.1"
                     /translation="MNRQTRIRLVASLPFLILMAGLFGSRNSMETLAQPTATPGASGY
                     HVAKTIPIGGDGGWDYVAADPVGRRIYISHATHVVVLDADTKEKVGDIPDTQGVHGIA
                     IASEFGRGFVSNGRSNNVTMFELKSLKTLGLIPVGTNPDAIIFDPATKRVFAMNGRSG
                     DITAINAADGKVLGTVAIGGKLEFAAADGKGTIFVNVEDKSELVHVDAEKLTVLHRWP
                     LAPCTEPSGLAMDTQTRRLFVGCDNKMMAIVNADTGKIITTLPSGEGTDASGFDPETK
                     FAFTSNGDGTLTVVHEDSADTFSVVENVVTKKSARTMTVDLNTHNIFLPAADFDAPGA
                     GERRGKMKPDSFVVLMLTR"
     gene            2704..>3209
                     /locus_tag="VFR42_10825"
     CDS             2704..>3209
                     /locus_tag="VFR42_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEU0369697.1"
                     /translation="MTAHESSSAKRALKPQRGPHAPKFIVIEGPLRVGKSTLARILAE
                     RLHARRIYDCEDNPFLGDFYKEAPGSALRAQMYFLFERQKRLREALAADLPSPLLSDF
                     LMEKDRIFANLNLDDDDLKLYERYYTALTNELPAPDLVIYLQAKPEVLRARIAKKAAK
                     DENQISQE"
BASE COUNT          671 a          915 c          896 g          727 t
ORIGIN      
        1 aaaacacagt actgagagta agcgtagctt ggaacagtca ccggatcacc agaaggttct
       61 actcagtccc cggggctgtt ggcgttccgg gttgagtgcg gagtaatcgg aaaattctct
      121 tatagggacc tggatcggtc acctaggaaa gtaaagacgc agcggaaccg tccagctgtc
      181 cttcttgccg gaatgggaac cggcccgaac cttccagctt ctggagtaga ggagaagagc
      241 acattcgcct ggcgacggaa aaaaccgtgg accataaaga ctatgtgaat cgcgccgttg
      301 aagcgtccat ccacatcggg ctggttcttc tactggccgc cacttgcttc cttatcttgc
      361 gcccattcct ccccttgatt gcgtggggca tcatcgtcgc gatcgccgtt tacccgcgct
      421 acagacagtt gcagctcgtg ctcggcggcc gtggcgtgct cgctgctgtc cttatcaccc
      481 tcttactact agctttgtta actgttcctg tggtcttgct cgccggaacg ctgattgacg
      541 gtatccagac cctcgcagca cacctcaaag acggaacccc aattatccca ccgccccccg
      601 ctggcgtcga aacctggcct ttcgttggcg ttccattgaa aagcgcatgg agcctggctt
      661 caacgaacat taccaccgcg ctaaggagtt tcgctcctca aatcagggcg atcgtccctg
      721 gcctgctctc ggcttctgcg ggaataggcc tcaccgttct gcaattcgct ctttccatca
      781 tagttgcggg agcccttctc gctaactcac ggggcgctgc cgctgctgcg cactccttag
      841 ccaaccgtat atttggcgac aaaggttcgg agtttgagca actcgtcggc tccaccatcc
      901 gcagcgtcac gataggaatc ctcggcgtag ctctgatcca atcggttctc gcggctgttg
      961 gttttctagt agcaggattg ccgggtgctg gcttgtgggc cgccgtgttt ctagttgccg
     1021 cactgctaca ggtggggacg ctggtcctta ttcccgccgt gatttacgtg ttcgcaacct
     1081 caagcgccac gaaagccata attttcatgg cttggtgcct catcgtcgcc ctgatagaca
     1141 atatcgtaaa gccccttctg ttaggccgag gcgtcgcagt tcccatcgcg gtcgtattcc
     1201 tcggtgcgat aggaggattc gtagcgatgg gccttatcgg cttgttcgtt ggcgccatcg
     1261 ttctgtcggt gggctacaag ttgttcattg cctggctagc tcgaactccc gccgtacttg
     1321 gggagacttg atatacagcg cggtcccttg ccggatcaag ctgacaggga gcgtgcggtg
     1381 tcgatttcac tcagacggca gtagttctgc aaatgctgtt cgtgtagggt aagccggaga
     1441 cgttccgggg acctgcgtcc ctaccgcgac attaatcggt acattcgtcg aagctgtggc
     1501 catgttaatc ggctgtttat ctgaagtacg ttagcgttcg catgacgatt tgcagcgatg
     1561 tgtgagtagg agacttcttc atgaacaggc agaccagaat ccgccttgtg gcttcactac
     1621 cttttctaat cctgatggcg ggccttttcg gttccagaaa ttcgatggaa actctcgcgc
     1681 agccgacggc gacccctgga gcaagcggct accacgttgc caagaccatt cccattggcg
     1741 gagacggcgg ctgggattac gtcgctgccg atcctgttgg ccgccgcatc tacatttctc
     1801 atgccacgca cgtagtagtg ctcgatgcgg acactaagga aaaagtcggc gacattccgg
     1861 acacgcaggg cgtgcacggc atcgccatcg catcggaatt tggccgcgga tttgtgagca
     1921 atggccgcag caacaacgtc accatgttcg aactgaaatc gctgaagacc ttggggctga
     1981 ttccggtagg aacgaatccg gacgccatca tttttgaccc ggccaccaag cgcgtgtttg
     2041 cgatgaacgg ccgcagcgga gacattacgg ccattaacgc cgccgacggc aaagtgcttg
     2101 gtaccgtggc gatcggcggc aagctggaat tcgcggctgc tgacggcaag ggtacgattt
     2161 tcgtaaacgt ggaagacaaa tcggaactgg tgcacgtgga cgccgagaaa cttacggtgt
     2221 tacaccgctg gccgttggcg ccatgcaccg agccttccgg cttggcgatg gatacgcaaa
     2281 cgcggcggtt gtttgtcggc tgcgacaaca aaatgatggc cattgtgaat gcggacacgg
     2341 gaaaaataat caccacgctc ccgagtggag agggaacgga cgcgagtggc ttcgatcccg
     2401 aaaccaagtt tgcgtttacc tcgaatggcg acggcacact caccgtggtg cacgaagatt
     2461 cggcggacac attttctgtc gtggaaaatg tcgtcaccaa aaagagtgcg cgaacaatga
     2521 ccgtagacct gaatacgcac aacatttttc tgccggcggc ggatttcgat gctcctgggg
     2581 caggagaacg gcgcggcaag atgaaacctg attccttcgt ggtgctgatg ctcacacgat
     2641 agaactttcc ttgctatact ctgatgcacg atcgctgcgc ttgtagcgcg gcgagaccaa
     2701 ccgatgacgg cgcacgaatc gtcctccgcc aaacgcgctc tcaagccaca gcgtggtccg
     2761 cacgctccca aattcatcgt gatcgaaggg ccgctgcgcg tcggaaaatc cacactggcg
     2821 cgcatcctgg ccgaacgcct gcacgcgcgg cgcatttacg attgcgaaga taatcccttt
     2881 ctcggcgatt tctacaagga ggctcccggc tcggcgttac gcgcacaaat gtattttctt
     2941 tttgagcggc agaagcgctt gcgcgaagcc ctggccgcag atttgccctc tccgttgctt
     3001 tccgacttcc tgatggaaaa agaccgcatc ttcgcgaacc tcaatctgga tgacgacgat
     3061 ttgaaactgt acgagcgcta ctacacggcg cttacgaacg aactgcccgc tcccgacttg
     3121 gtgatttacc tccaggccaa gccggaagtc ttgcgcgctc ggattgcgaa gaaggcggcg
     3181 aaggacgaaa accagatttc tcaggaata
//
LOCUS       DASTPM010000615         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_624901, whole genome shotgun sequence.
ACCESSION   DASTPM010000615 DASTPM010000000
VERSION     DASTPM010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624901"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VFR42_10830"
     CDS             <1..954
                     /locus_tag="VFR42_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369698.1"
                     /translation="AGQPELDERLRQPELRQLRQRIAVRCRIPLLTLEETEEYLEARL
                     KGAGCVNRGIFPVESIQSLYSYAQGIPRVTNLLCEKALMAAYAERHTVVSPVNVRHAA
                     AELDFAYEPRPGPVPEISLRPGIVVPMDPPAPEPHVDEPVDNPSPLPLYQEAPAHAVA
                     APAHTLAEPSSQPQPSPPLPAMQLPTSIPEARTKPNIRVFAPKPPVYEPPLAEPPAKA
                     AQPKANLAPKPARRVSRPQSAFRRYWSDVARSFVRDCRYFFSAFRTQSDPDGKVLFMK
                     KYDLRRDLVAPVSRWLSKPVNLKGSNSRAADSERRASRSHF"
     gene            complement(1119..2345)
                     /locus_tag="VFR42_10835"
     CDS             complement(1119..2345)
                     /locus_tag="VFR42_10835"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEU0369699.1"
                     /translation="MITVLLPANAADYESHLQGLLGSGTLPPFDPVLVDFVDAVSKAV
                     LLDPVLRRMPEMAAVAHWMRKAHILDLRRSFEAQRGERIWLPRGTALHFAPSNVDSIF
                     VYSWFISLLTGNQNVIRLSQSRGEQVEFLLATINRVCGQERFQPIRDRSLVLSYDHDD
                     AVTQHLSETCQIRVLWGGDDSVRRLRAVPLNPLATEIAFANRFSLAVLNSVSVAAASD
                     DALANLAMRFSNDSFWFDQMACSSPRLVVWIGALGDCKQAQNAFWPALTREVRKRGIE
                     YAEVIGLNKMVSAYVSAAAEISDQILPGVTGVVSRLHVGKNAKGRFRSIECGGGLFFE
                     TELSHLDRLAPCLTAQDQTLSYFGFGPADLTQFALNLPARAVDRIVPIGAALEFNTVW
                     DGYNLLQVFTREIDLR"
     gene            complement(2342..>3024)
                     /locus_tag="VFR42_10840"
     CDS             complement(2342..>3024)
                     /locus_tag="VFR42_10840"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369700.1"
                     /translation="GVLKDRSAFSARAAGILGFSQFGSGHTYVLRDETLEADWNKIDQ
                     FLDRHRGERILLFGFTFLVWRNLLRAAEESGKRLKFENSVLIHGGGWKLLSDQKVSND
                     EFKRRLRKSLGIQAIYNYYGMVEQTGSICMECEAGYFHPADYSEILIREPRTLREQEA
                     GIPGLIETLSTIPRSYPGHVLLTEDLGIVHGTDGCSCSRRGTYFTVLGRLASAELRGC
                     SDTQQAAS"
BASE COUNT          699 a          930 c          823 g          572 t
ORIGIN      
        1 gccggccagc cggagttgga cgaaagactg cggcaaccgg aactccgcca gttacgccag
       61 cggattgcgg tccgctgccg gattccgctc ctcacactcg aagaaactga ggaatacctg
      121 gaagcgcgcc tgaagggtgc gggttgcgtg aaccgcggga tcttcccggt ggaatccatc
      181 caatcgcttt attcttacgc gcaaggcatt ccgcgcgtca ccaatctcct ttgtgaaaag
      241 gctctaatgg ctgcctatgc cgagcggcac actgtcgtct ccccggtcaa tgtcaggcac
      301 gcagcagccg aactcgattt tgcctacgaa ccaaggcccg gccctgttcc ggagatctcg
      361 ctgcggccag gcattgtggt tcccatggat ccgccggcgc ccgagccgca tgtagacgag
      421 ccagtagata acccatcccc gctcccgctt taccaggaag cgccggctca tgccgtggca
      481 gcaccagctc ataccctggc cgagccgtca tcgcaaccgc agccgtcgcc gccgctaccg
      541 gctatgcaac tacccacatc cattcccgag gcgcgcacga agcccaatat cagggtcttc
      601 gcccccaagc cgcccgtgta cgaaccaccg ctggcagaac cacctgctaa agccgcccag
      661 ccgaaagcaa atctggcgcc aaagccagcg cgaagagtgt ctcggcctca gagtgctttc
      721 cgacgctact ggagtgacgt cgcgcggtca ttcgtgcggg attgccgata ttttttctca
      781 gccttccgta cgcaatccga cccagatggc aaagtcctgt ttatgaaaaa atatgattta
      841 cgccgcgatc tcgtcgcgcc cgtgtcacgc tggctcagca agcctgtaaa cctcaagggc
      901 tcaaactcgc gcgcagcgga ttcagaacgc cgcgccagtc gaagccactt ttgagcgcaa
      961 gtgcgtcgac ataaattact tgccgtgcga ggcggccgcc ggcatctgtt tcgagagttt
     1021 gcgggaacct gaaaacgata agtgcctggc gacagacgaa agagcaattg gatcggcgtt
     1081 gcgccgcaaa tctcaaattc attcgcccgc acggagaact agcgcagatc aatttcgcgc
     1141 gtgaagacct gcaacaggtt gtatccatcc cagacagtat tgaactcgag agccgctcca
     1201 atcggcacaa tgcggtccac agcgcgagcg ggaaggttca gtgcaaactg cgtcagatcc
     1261 gcagggccga aaccaaagta ggaaagcgtc tgatcttgcg ccgtcaggca aggggcgagt
     1321 cggtccagat gcgaaagctc tgtttcaaag aacaggccgc cgccgcattc gatgctgcgg
     1381 aacctcccct tagcattctt gccaacgtgc aggcggctca cgactcccgt aacaccaggg
     1441 agtatctggt cggaaatttc tgcggcggcc gagacgtagg cggaaaccat cttgttcagg
     1501 ccgatgacct cggcgtactc aatcccgcgt tttcgcactt cccgcgtcag ggcaggccag
     1561 aatgcatttt gcgcctgctt gcagtccccc aacgcaccta tccagacaac cagacgaggc
     1621 gaagagcatg ccatttgatc aaaccagaag ctatcgttag agaagcgcat cgccaggttc
     1681 gccaaggcgt cgtcactcgc cgcagcaacc gaaacggaat tgagcaccgc aagcgaaaag
     1741 cggttcgcaa aagcgatctc agtggctagc gggttcaagg gaacagcccg taggcgtcga
     1801 acgctgtcat cgccacccca caacacgcgg atttgacagg tttccgaaag atgttgcgtc
     1861 accgcatcgt catgatcata ggataggacg aggctgcggt cacggatcgg ctgaaatcgt
     1921 tcttggccac agacgcgatt gatggttgcc agcaagaact ccacttgctc gccccggctt
     1981 tgggacaggc gaatgacgtt ctggttgccg gtcaatagcg aaatgaacca ggagtagacg
     2041 aaaatcgaat ctacgttcga aggagcaaaa tgcagtgccg tgcccctggg cagccaaatg
     2101 cgttctccgc gctgcgcttc gaacgaccga cgcaaatcta ggatgtgcgc ctttcgcatc
     2161 caatgcgcaa ccgccgccat ttccggcatg cggcgcaaga ccggatccag caacacggct
     2221 ttggatacag catcaacgaa atccaccagg accgggtcga atggcggaag tgtgccggat
     2281 ccaagcagac cctgcaggtg tgattcgtaa tccgcggcat tcgccggtag gagaacggta
     2341 atcacgaggc cgcctgttga gtatcgctgc agccgcgcaa ctccgcactc gcgagacgcc
     2401 caagtacagt gaagtacgtc ccgcggcgcg aacaactgca tccatcggtt ccatgcacga
     2461 ttcccaagtc ttccgtgagc aacacgtggc cagggtagct tcgtggaata gtactgaggg
     2521 tttcgatcaa gcccggtatc cctgcctcct gttcccggag agttcgcggc tcgcggatca
     2581 gaatttcgga ataatccgcg ggatgaaaat atcccgcctc gcactccatg cagatagaac
     2641 cggtctgctc taccattccg tagtagttat atatcgcctg gatgccgagc gatttacgaa
     2701 gccgtcgctt gaactcatcg tttgagacct tctggtcaga aagcaacttc catccgccac
     2761 catgaatgag aacgctattt tcgaacttga gcctcttgcc gctctcctcg gcggcgcgaa
     2821 gcaaatttcg ccagacgaga aaagtgaatc caaacagcaa aatacgctca ccccggtgtc
     2881 tatccaggaa ctggtcgatc ttgttccaat ccgcctccag ggtttcgtcg cgcagcacat
     2941 acgtatgacc cgagccgaac tgcgagaaac caagaatgcc ggccgcgcgt gcggagaatg
     3001 cgctccggtc cttcaaaacg ccgg
//
LOCUS       DASTPM010000616         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_224304, whole genome shotgun sequence.
ACCESSION   DASTPM010000616 DASTPM010000000
VERSION     DASTPM010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224304"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFR42_10845"
     CDS             <1..579
                     /locus_tag="VFR42_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEU0369701.1"
                     /translation="SVKLTGDGLQMAGQEQGMNIYQRASVAAGVLVSVTLSGTAPPPA
                     ASGADAGGAPGRAENVQGGEAAGTIQSIPGRLDALKWPLVGGFCILFLLAAILLARKP
                     VAIAVPPQVGSASGAPVSGPTTKSPDKRTRASGNGDALASLDRANTNSLDVLKDQLFR
                     LELRRQAGTISEEEYSGERAKAEQVLRELVRG"
     gene            583..1278
                     /gene="ccmA"
                     /locus_tag="VFR42_10850"
     CDS             583..1278
                     /gene="ccmA"
                     /locus_tag="VFR42_10850"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEU0369702.1"
                     /translation="MPAAPVRSIGLQFDQVDKRYGAVYALRDLTLNIAAGECVAVVGR
                     NGSGKTTLLRAAAQLVRPSRGSIAFSGGAEDAKAAIGFVAHATMVYDELTAEENLVFF
                     ARLQELAEPKTRADELLREVGLHDRKDSLVRTFSRGMRQRVAIARALLAQPSLLLFDE
                     PATGLDAESNLWLTEHLRKLRDTGCTILMSLHGDSELARLATRAIRLEGGGLIADTRR
                     GAMFQSIFAAAGY"
     gene            1279..1953
                     /locus_tag="VFR42_10855"
     CDS             1279..1953
                     /locus_tag="VFR42_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU0369703.1"
                     /translation="MALLSNAAVLLGKEVRTEFRSRELLSTTIVFVLIVVVVFSFTFD
                     PTTNESRRFGPGLLWMAFLFAASLMLQPCFLREQVNDTLGALRLAVSDPFAIFLAKIC
                     ANTLFLLLTELLMLPVFAALFNVSFGAQLPGILLTFLLGSLGISVVGTALAVISAQAR
                     MRELLLPLLLLPLLTPILVVSTEVTIGLMQPEPYMEWKGIGLMAGFDVVFLTVLWLFG
                     EYLLEE"
     gene            1963..>2592
                     /gene="ccsA"
                     /locus_tag="VFR42_10860"
     CDS             1963..>2592
                     /gene="ccsA"
                     /locus_tag="VFR42_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU0369704.1"
                     /translation="MMKRVLFGVVAIGLVACAAYASFYIAPEERTMGAIQKIFYFHAS
                     TAWAGETTFFVCFLANLLYIWKRQPQFDWLGVTCAEVGVACITVVLITGPIWAKPVWG
                     IWWTWDARLTSTFVLWILYICYLLLRTLVDEPDRRALLSALFGVFAFIDVPIVFGAIR
                     WWRTQHPAPVIMGGPGSGLEPTMKAVFFFNVAVMHVLAAFLIMERYSLEK"
BASE COUNT          440 a          718 c          840 g          594 t
ORIGIN      
        1 agcgtgaagc tgaccggcga cggcttacag atggctgggc aagagcaggg aatgaacatt
       61 taccaacggg ccagcgtagc ggccggagtc ttggttagtg tgactttgtc cggaacagcg
      121 ccgccgccag cggcttcggg agcggatgcc gggggtgcgc cgggccgcgc ggaaaatgtg
      181 caaggcggcg aagcagccgg aacgattcag tctatcccgg gacggctcga cgccttgaag
      241 tggccgctag ttggcggatt ctgcattttg ttcttgctgg cggcgattct gttggcgcgc
      301 aagccggtgg cgattgcagt gccgccccaa gtagggtcag catcaggagc gcccgtgagc
      361 ggcccaacca caaagagtcc tgacaaacgg acgcgagcct cgggaaatgg ggacgcgctc
      421 gcttcactcg accgcgcgaa cacgaacagc ctcgacgtcc tgaaagatca gttgttccgg
      481 ctggagctgc gccgccaggc cggtaccatc agcgaagaag agtactcggg cgagcgcgcc
      541 aaagccgaac aggtgctgcg ggaactggtg cgaggctaag ccttgcctgc cgcgccggtt
      601 cgctccatcg gactgcaatt cgaccaggtg gacaagcggt atggcgcggt ctatgcgttg
      661 cgcgacttga cgctgaatat cgccgccgga gaatgtgtgg cggttgtcgg gcgcaacggt
      721 tccggcaaaa ccacgttgct gcgcgccgcg gcgcagctgg tacggccttc acgtggatcg
      781 atcgcctttt ctggcggcgc ggaagatgcg aaagccgcaa tcggatttgt agcccatgcc
      841 accatggtgt acgacgaatt gactgccgaa gaaaatcttg ttttctttgc cagattgcag
      901 gaactcgcgg aaccaaagac tcgcgccgat gaactgctgc gagaggtggg gctccacgac
      961 cgcaaggatt cactggtgcg tactttttcg cgcggcatgc ggcagcgggt ggccattgcg
     1021 cgggcgctgc tggcgcagcc ttcgctgctg ctgtttgacg agccggcaac tggcctggac
     1081 gcggaatcga acctgtggct gaccgaacac ttgcgcaagc tgcgtgacac cggctgcaca
     1141 attctgatga gcttgcatgg cgattccgag ttggcgcggc tggccactcg cgccattcgt
     1201 ttggaaggcg gcggcctgat tgccgatacg cggcgcggcg ccatgtttca atccattttt
     1261 gcggcggcgg gctactgaat ggccctgctg tcgaacgcgg cggtgttgct cggcaaggaa
     1321 gtgcgcaccg agtttcgctc gcgcgagctg ctgagcacca ctatcgtctt cgtgttgatc
     1381 gtggtggtgg tgttcagctt tacttttgat ccgaccacca acgaatcgcg ccggtttggg
     1441 ccgggattgc tgtggatggc cttcctgttt gcggcttcgc tgatgctgca gccgtgcttt
     1501 ctacgcgaac aagtgaacga cacgctcggc gcgctgcggc tggcggtcag cgatcccttc
     1561 gcgatttttt tggccaagat ttgcgcgaac acgctgtttt tgttgctgac cgaattgctg
     1621 atgctgccgg tgttcgcggc tctgttcaat gtgtcgttcg gagcgcaatt gccaggcatc
     1681 ctactgacgt tccttctcgg cagcctgggt atttccgtcg tgggcacggc tctggcggtg
     1741 atttccgccc aggcgcgtat gcgcgaactg ttgctgccgc tgctgctcct gccgctgctt
     1801 acgcccattc tcgtggtcag cacagaagtg accatcggcc tgatgcagcc ggagccgtac
     1861 atggaatgga agggcatcgg cctgatggcc ggctttgatg tagtgttttt gacggtgctg
     1921 tggcttttcg gcgaatattt attagaggaa tagtccagcc gcatgatgaa acgtgttctt
     1981 tttggcgtgg tggcgattgg cctggtggcc tgcgcggcct acgcatcctt ttacattgcg
     2041 ccggaagaac ggaccatggg cgcgattcag aagatttttt attttcacgc atccacagct
     2101 tgggcgggcg agactacctt ctttgtctgc ttcctcgcta acctgcttta catctggaag
     2161 cgccagccgc aattcgactg gctcggcgtc acgtgcgccg aggtcggcgt cgcgtgcatc
     2221 acggtagtgc tgattaccgg gcccatctgg gccaagcccg tgtggggtat ctggtggact
     2281 tgggacgcgc ggctgacgtc caccttcgtg ctttggatcc tgtacatctg ctatctcctg
     2341 ttgcgcactc tggtagatga accagatcgt cgggcgctgt tgagtgcgct cttcggcgtg
     2401 tttgccttca tcgatgtgcc aatcgtattt ggcgcgattc gttggtggcg cacgcagcat
     2461 cccgctccag taattatggg cggaccgggt tccgggctgg aaccgaccat gaaggcagtt
     2521 ttctttttca acgtcgcggt gatgcatgtg cttgcggcat ttctgatcat ggaacggtat
     2581 tcgctcgaaa ag
//
LOCUS       DASTPM010000617         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_685331, whole genome shotgun sequence.
ACCESSION   DASTPM010000617 DASTPM010000000
VERSION     DASTPM010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685331"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..701
                     /locus_tag="VFR42_10865"
     CDS             153..701
                     /locus_tag="VFR42_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0369705.1"
                     /translation="MSNSPRVDLLERDAASPDAQKIYDALLATRGVVPNMFKVLAQRP
                     EIAQGVAGFLKPLLSDGALPGLYKELIAVRVSQLHGSAYAIKAHSISARQKGATEAQI
                     EAVANFEGGPYTEAEKLGFRLADRLHQGAASVDDEFYSTLAASFSEPERIELFLTAAA
                     FEMFPRFIDGLRIPVTSPPAGK"
     gene            728..1909
                     /locus_tag="VFR42_10870"
     CDS             728..1909
                     /locus_tag="VFR42_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0369706.1"
                     /translation="MARIATRVSQLNGEGALAVFSRAKELERQGRSIIHLELGEPGFH
                     PAAPVIDSLRNAVAAGRDRYCATRGIPALREEITHYLARTRKLSLKPEQVLVAPGCKM
                     ALSLAMMALIEPGDEVLYPDPSFPIYPSFTRGLGAKAVPFALREENKFQPDLAEIAAK
                     IAAKTRVLIFNSPNNPTGTVYDDRTVEGIAELARRHDLWVITDEIYARIVFGGGFKSI
                     WNLAGMPERTIIIDGFSKSFAMTGWRLGYAVAPVYVIDAMDLLVLNTFTCAAEFTQVA
                     AIEALRDTTNAVDAMVAEYKLRRDLFLKSLAKIPGFRCHSPAGAFYAWVNIEDTGLAA
                     EEVQKILLEEAGVAAIAGAAFGAEGKNYLRFSLVSARNLLEEALDRIQRVSVKWRAVP
                     A"
     gene            1999..>2232
                     /locus_tag="VFR42_10875"
     CDS             1999..>2232
                     /locus_tag="VFR42_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369707.1"
                     /translation="MKRLFHFSGVALLALAVAGSAASQSSPYPTADVQKLYQDLLGKI
                     NAIPLYDNHAHPGFADDTDVDAMAAPPDESSVLR"
BASE COUNT          440 a          754 c          571 g          467 t
ORIGIN      
        1 gctcttttct ttggtggccc tctcgaccat cagctcgcta agcgcatcgc ctgcacccca
       61 ccacatgacg aatcctgctc ccaacggtaa actactaacc gcaaacaatt taacgaaatc
      121 ttaggccttc acccgctgct ccggagacgc acatgtccaa ttccccgcgc gtagacttgc
      181 tcgaacgcga cgctgcttcg cctgacgccc aaaaaattta cgacgccctt ctcgccaccc
      241 gcggtgtcgt tcccaacatg ttcaaagtcc tcgcccagcg ccccgaaatc gcgcaaggcg
      301 tcgccggatt tttgaagccg ctgctctccg acggcgctct ccccggcctc tacaaggagc
      361 tgatcgccgt ccgcgtctcg cagcttcatg gcagtgctta tgccatcaag gcgcacagca
      421 tttccgctcg ccagaaagga gccacggaag cgcaaatcga agccgtcgcc aatttcgagg
      481 gcggccctta cacggaagca gaaaaactcg gcttccgcct cgccgatcgc ttgcatcagg
      541 gcgcggcttc cgtcgatgac gaattctact ccaccctcgc cgcttccttt tccgaacctg
      601 agcgcatcga actgtttctc accgccgccg cgtttgaaat gtttccccgc ttcatcgacg
      661 gcttgcgcat tcccgtcacg tctccgcccg cgggcaaata ggggaaagca gtgttaaact
      721 tttttgcatg gctcgcatcg caacccgcgt ttcacaattg aatggcgaag gtgccctcgc
      781 cgttttctct cgcgccaaag aactcgaacg ccaaggccgc tccatcattc atctcgagtt
      841 gggcgagccg ggttttcatc ccgccgctcc cgtcatcgat tcgctgcgca atgcagtcgc
      901 cgccggccgc gatcgctact gcgccacccg cggcattcct gcgctgcgcg aagaaatcac
      961 gcactacctg gcgcgcaccc gcaaactttc actgaagccg gaacaagttc tcgtcgcgcc
     1021 gggatgcaag atggctcttt cgctcgcgat gatggcactc atcgaacccg gcgacgaagt
     1081 cctttacccc gatcccagct tcccgattta tccgtcgttt actcgcggcc tcggcgccaa
     1141 agctgttccc ttcgcgctgc gcgaggaaaa caaattccag ccggacctcg ccgaaatcgc
     1201 cgcaaaaatc gcggcgaaaa cgcgcgtctt gatcttcaat tcgccgaaca atcccaccgg
     1261 caccgtctac gatgaccgga ccgtcgaagg cattgccgaa ctggcgcgcc ggcacgatct
     1321 gtgggtcatc accgatgaaa tctacgcgcg cattgtattt ggcggcggat tcaaatccat
     1381 ctggaatctt gcaggaatgc cggagcgcac catcattatt gacggttttt cgaagagctt
     1441 cgccatgacc ggctggcggc tgggctacgc cgttgctccc gtttacgtga tcgacgcgat
     1501 ggacttgctc gtcctcaaca ccttcacttg cgccgcggaa ttcacgcagg ttgccgccat
     1561 cgaagctctc cgcgatacca ccaacgcggt ggacgccatg gtcgccgaat ataaattgcg
     1621 ccgcgatctt ttcttgaagt cgctcgccaa aattcccggt ttccgctgcc actcgcccgc
     1681 aggcgctttc tacgcctggg tcaatattga agacaccgga ctcgccgccg aagaagtcca
     1741 gaaaattctg ctggaggaag ccggcgtcgc cgcgattgcc ggagcggcct ttggcgcgga
     1801 agggaaaaac tatctgcgct tctcgctggt cagcgcccgc aaccttctcg aagaggcgct
     1861 cgaccgcatc cagcgtgtct ctgtaaaatg gcgtgccgtt cccgcataag ccgggcggtc
     1921 cgcttttact ccggtactgt agaagcggcc tttccagcgg cgggcggaca tggcgttgtg
     1981 cgacaagagg aatgcaagat gaaacgtttg tttcacttct cgggtgttgc cctgctggct
     2041 ctggcagtgg caggatccgc cgcctcgcaa tccagcccct atcccaccgc tgacgtgcag
     2101 aagctctatc aggacctgct cggcaaaatt aacgccatac cgctttacga caaccatgcc
     2161 catcccggct ttgccgatga caccgatgta gacgccatgg ccgctcctcc ggatgagagc
     2221 agcgtcttgc gc
//
LOCUS       DASTPM010000618         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_79543, whole genome shotgun sequence.
ACCESSION   DASTPM010000618 DASTPM010000000
VERSION     DASTPM010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79543"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VFR42_10880"
     CDS             complement(<1..1056)
                     /locus_tag="VFR42_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369708.1"
                     /translation="MTEMAGKANTEALTAALRKVAAFADLSQADLEWFIGHAEERRLK
                     PGEIVTKEDSPAELMLVILEGEMRGRRESDGPDSQGYTVQGPAVTGYLPFSRMKKVPM
                     TVRPVTPLHLLSLHSAHFPEMLHRMPELAQRLVGVLTDRVRNFTMVSQEREKLAALGK
                     LSAGLAHELNNPSAAARRSAGALRDCLARLRQVGRSSTIGPDDCAKLAKREEEIRSAL
                     KPAQFKDEFERVEREESVQGWLEQRKVPEAWKLAPLLVEANLTDTQLESFASVAGASL
                     GPELTRFATLLEMERIASELENSTARISDLIKAIKEYSYMDQAKLQEVDITKSLETTL
                     TIMHHKLKRGIAVTRNYA"
     gene            complement(1103..>2210)
                     /locus_tag="VFR42_10885"
     CDS             complement(1103..>2210)
                     /locus_tag="VFR42_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0369709.1"
                     /translation="LGPEAETLPLILFPDGERLAEPQFPAVASKLGLHTKAQTNFYDL
                     AIIGGGPAGLAAAVYGASEGLRTVIVEREAPGGQAGLSSRIENYLGFPSGLSGLDLAR
                     RAVAQARRFEVEILSPQEAKGLRIDGPYRHITLSDGTEISCHALLLAMGVQWRALDVP
                     GIDRLQGAGVYYGGGASEAIACKGETVYIVGGANSAGQAAMHFSKFAQKVIMLVRGGS
                     LASTMSHYLIEQIEKTNNIEVRTHTSVVEVHGDTHLSEITIQRAVTAETERLPATSLF
                     IFIGAQPRTQWLDGLIMRDELGFVLSGPDLMKDGKRPEGWTLDRDPGLLETNVPGVFA
                     VGDVRHGSVKRVASGVGEGAVVVQFMHQYLAKVQ"
BASE COUNT          406 a          745 c          597 g          462 t
ORIGIN      
        1 tgcgtagttg cgggtcaccg caattccgcg cttcagtttg tgatgcatga tggtcagtgt
       61 ggtttccagg ctcttggtga tgtccacctc ctgcagcttg gcctggtcca tgtacgaata
      121 ttccttgatg gctttgatga gatcggagat gcgcgcggta ctgttttcca gttcgctggc
      181 gattcgctcc atctccagca gcgttgcgaa gcgggtcaac tccgggccta acgaggcgcc
      241 cgctacgctg gcaaagcttt ccagttgtgt gtccgtcaag ttcgcctcga ctagcagcgg
      301 cgccagtttc caagcttccg gaactttgcg ctgttccagc cagccctgaa ccgattcttc
      361 gcgttcgacg cgttcgaatt catctttgaa ttgcgccggc ttcagtgcgc tgcgaatttc
      421 ttcctcacgc ttggccagtt tcgcgcaatc gtcgggacca atggtcgaag agcggcctac
      481 ctgccgcagg cgcgcgagac aatcgcgcaa agctcccgcg gaacgccgcg ccgccgccga
      541 tggattattc aattcgtgcg ccaggcccgc ggagagtttg ccgagcgcgg ccagcttctc
      601 acgctcctgg ctaaccatgg tgaaattccg gacccggtca gtcagtaccc cgaccaggcg
      661 ttgcgccagt tccggcatcc gatgcagcat ctccggaaaa tgcgcggagt gcagggacag
      721 caggtgcaac ggcgtaaccg gtcgcacggt catcggcact ttcttcatgc gtgaaaatgg
      781 cagatatccg gtgaccgccg gcccctgcac cgtatatccc tggctgtccg gcccatcact
      841 ctcgcgccgc ccgcgcatct cgccttccag tatcacgagc atcaattcgg ccggcgaatc
      901 ctccttggtc acaatctcgc cgggcttgag gcggcgctcc tcggcatggc cgataaacca
      961 ttcgagatcg gcttgtgata ggtccgcaaa ggccgccact ttgcgcaggg cagcagtaag
     1021 ggcttccgtg ttggccttgc ccgccatctc cgtcatgagc tttgccgcct ctccgaatcc
     1081 gttctacttc gccgcgaccg gctcattgca ctttcgccaa gtattgatgc atgaactgca
     1141 ccaccaccgc gccttcgccc actccggacg ctacccgttt cacggaccca tgccgtacgt
     1201 cacccactgc gaagacgccc ggaacatttg tctccagcaa tcccggatcg cggtccagcg
     1261 tccagccttc cggccgcttg ccatccttca tcagatccgg gccggacagc acgaatccga
     1321 gttcgtcacg cataatcaag ccgtccagcc attgcgtgcg cggctgtgcg ccgataaaga
     1381 taaatagact ggtggccggc agtctctccg tttccgcggt aaccgcgcgc tgaatcgtaa
     1441 tctccgatag atgcgtatcg ccatgaacct ccacgacgct ggtgtgcgtc cgtacctcaa
     1501 tgttgttcgt cttctcgatt tgctcgatca agtagtgcga catggtgctg gccagcgagc
     1561 cgccgcgcac cagcataatc accttctgcg cgaatttcga gaaatgcatc gcggcttgcc
     1621 ccgcggaatt cgcgccgccc acgatataaa ccgtctcgcc cttgcacgca atcgcctccg
     1681 acgcgccgcc gccgtaatat actcccgcgc cctgcagccg atcgattccc ggaacgtcca
     1741 gcgcgcgcca ttgcacgccc atggccagca acaatgcgtg acaggaaatc tccgtcccat
     1801 ccgaaagtgt aatgtgccgg tacggaccgt cgattcgtaa gcctttcgct tcttgcggcg
     1861 aaaggatctc cacctcaaag cgacgcgcct gggccacagc acggcgcgcc aaatcaaggc
     1921 cgctcagccc tgaaggaaat cccaaatagt tttcaatgcg cgaactcagt cccgcttgcc
     1981 cgcccggggc ctcgcgctcc acaatcaccg tgcgcaatcc ttccgacgct ccgtaaacgg
     2041 cggcggccag gccggccggt ccaccgccga tgatcgccaa gtcataaaaa ttggtctgcg
     2101 ccttggtgtg caggcccagt ttgctggcta ccgccggaaa ttgcggctcg gccaggcgct
     2161 caccgtcggg gaaaagaatc agcggcaaag tttccgcttc cggccccagc
//
LOCUS       DASTPM010000619         5129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1435192, whole genome shotgun sequence.
ACCESSION   DASTPM010000619 DASTPM010000000
VERSION     DASTPM010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435192"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFR42_10890"
     CDS             <1..486
                     /locus_tag="VFR42_10890"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU0369710.1"
                     /translation="EKGAGVWVDGNYVGYLKELKGKKKVLLLPGQHEVVVRQSGYLDS
                     VQKVVVQPGEKRLVIVTLQLAPHATVPDVTAKLKLNIKPKRAAVFLDEKYVGHASEFG
                     GKFRSMEIGPGKHKIRVELPGYRTFVTEVDLLANQETEVKTELQVGSIQQNTPIIKKP
                     D"
     gene            complement(580..1347)
                     /locus_tag="VFR42_10895"
     CDS             complement(580..1347)
                     /locus_tag="VFR42_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEU0369711.1"
                     /translation="MLTAITRAVSPSLSQCELSFIPRQPIDLGKAQSQHHAYEKLLAE
                     LGARVISLPAERELPDSMFVEDPALVLDELAVIFPLGTESRRREAPSLAAALQPFRKL
                     QHVRLPGMLEGGDILRLGRKLFAGLTARSNADGIEQLSAMVSPYDYQVFGVPVPGCLH
                     LKSAVTALDENTLLANRAWFDSSYFSGYDWLDVDPREPHAGNALTLSGTVIFPASFPR
                     TRARIEARGFTVATLDISELQKAESGLTCSSLLFDAA"
     gene            1454..2905
                     /locus_tag="VFR42_10900"
     CDS             1454..2905
                     /locus_tag="VFR42_10900"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0369712.1"
                     /translation="MRTEKRVRMVRFVVLVAIAAAGWSEAARAQDKKIAAVDEVFVDV
                     AKPGSPGCALAVARGGKIIYEKGYGLANIEENIRITPQSVFDIGSTSKQFTAASILLL
                     EKQGKLSVNDDIRKYLPEIPDYGHKITILHLLNHTSGLRDYLTLFELAGINTDSVTTD
                     EDALAIIANQKALNFEPGSEWLYSNSGFFLLSGIVKRVSGKTLTEFAAENIFAPLEMK
                     HTVYRDSHTLIVPNRALAYDVKEKEEGYSINVSYFEQTGDGAVHTSVEDLLKWDENFY
                     SGKVGGKEFLVELQEPGKLNSGKPLTYAKGLMVEKYRGLPIVQHGGSWGGYRAQLLRF
                     PEQHFSVVCLCNVGNAGPAQRAQRVADIYLAEEMKEPAAREKEGRKREKIETVAVAAE
                     KLAGYAGSFHSEELRATYRLVVEDGKLKLAGIQSGDGFLHSPQHVELRPVGPDLFVVE
                     EEGLQFAFEHNGANVSGFRLDAGRTKGIGFQRK"
     gene            complement(2911..3513)
                     /locus_tag="VFR42_10905"
     CDS             complement(2911..3513)
                     /locus_tag="VFR42_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0369713.1"
                     /translation="MTANLIPSVDPLMDTESLQEIASLPRYVRFGPFQIDRQRQQVYR
                     GGSKLRLQGKVYQVLLVLIHNQGEVVSREQLKHALWPADTHVNYDANVNTTVNKLRQA
                     LGESTEKPTYIETIPRRGYSFIGTAEFSAAPFPQETRDAAAAKPAESGELPGSSNAPA
                     ADGSRKWLTLVIIGLILAGILLGAGAATLWLRHFSPQLRH"
     gene            3956..>5129
                     /locus_tag="VFR42_10910"
     CDS             3956..>5129
                     /locus_tag="VFR42_10910"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0369714.1"
                     /translation="MRRLTVMALAAMLCVLLSSVTRAQRLDGTLRVTVTDHSQATIEE
                     AKVTAQNEATGVATATSASSAGTYVFPNLLVGTYTVTVEKDGFKKAVEKGISVGSNQV
                     AEAKVELEVGSVSAVVEVEAGAELVKTQSSDLDTTFGGKIASDLPINTVGGSVLEFAV
                     FAPGTTTQQGGVAGSGGSIGGNRPRFNGFSIDGVDDNRTDVNGPATPVIQDSVAEFTL
                     ITNQFSAEYGHSAGGQFSIVTKSGTNNFHGQAHGYNQNRNYNAMDNLQKDRGHQDRFD
                     YNRAGGDFGGPIKRDKLFFFGAYEYQNNGLASSSPTVQVPTQNGLAQILAQNPDAAVQ
                     AALAQFPIAPTKTGTVTVNGQPIDVGTFQSIAPSYFNQNDLNANIDANLGKNQIRAR"
BASE COUNT         1249 a         1223 c         1705 g          952 t
ORIGIN      
        1 gaaaagggcg ccggagtgtg ggtggacggc aactacgttg gctatctgaa agagctgaag
       61 ggcaaaaaga aagtgctgct gctgccgggg caacacgagg tggtggtgcg gcaatcggga
      121 tatctggaca gcgtgcagaa ggtggtggtg cagccaggcg agaagcggct ggtgatcgtg
      181 acactacaac tggcgccaca cgcgaccgtg cccgacgtta cggcgaagtt gaagctgaat
      241 atcaaaccaa agcgagcggc agtttttctg gacgagaaat acgtcggaca tgcgagcgag
      301 ttcggaggga aattccgttc aatggagatc ggccctggca aacacaagat tcgagtggag
      361 ctgccggggt accgaacatt cgtgaccgaa gtggatctgc tagcgaatca ggagacggaa
      421 gtaaagacag agttgcaggt ggggagcatc cagcagaata cgccgattat caagaagccg
      481 gactgagtga gatagtggtt agttcccggt tttcagtgat ccgcaagaga gaggtcagcg
      541 ccgctggtgg tttggcagcc gctgcgcccc gcaaaacgat caggctgcgt cgaacagcag
      601 gctggaacaa gtgagaccgg attcggcttt ctgcaattcg gaaatgtcga gtgtggcgac
      661 ggtgaagccg cgagcttcga tacgcgcacg agtgcggggg aaggaagccg ggaaaataac
      721 ggtgccggag agagtgagtg cgttgccggc gtgaggctcc ctgggatcca cgtcgagcca
      781 gtcatatcca gagaaataag aggaatcgaa ccaagcgcga ttggcgagca gggtgttctc
      841 gtcgagtgcc gttacggcgg actttagatg gagacaaccg ggcacgggaa cgccgaagac
      901 ctggtagtcg tatggcgaga ccatggcgga gagttgctcg atgccgtccg cgttgctgcg
      961 ggcggtgagt ccggcgaaca gtttgcggcc gaggcggagg atatcgccgc cttccaacat
     1021 gcccggaagc cgcacgtgtt gcagcttgcg aaaaggctga agggcggccg cgagagatgg
     1081 tgcttcgcgg cggcggcttt cggtgcccaa gggaaaaatc acggcgagct catcgagcac
     1141 tagggcggga tcttcgacaa acatggaatc gggcagttcg cgctcggcgg gaagcgaaat
     1201 gacgcgagcg ccgagctcag cgaggagttt ttcgtaggcg tggtgctgcg attgcgcctt
     1261 gccaagatcg atgggctggc gcgggatgaa gctgagttcg cattgagaaa gcgacggact
     1321 gacggcgcgc gtgatggcgg taagcattcg ggagaatgta tcagacgaag tatggatacg
     1381 aaagcgatag cgcagttgcg caggagaatt ggtgcggata gactccgatg cgtttcagga
     1441 caggagctat ggaatgcgga ctgagaaacg cgtgcggatg gttcggttcg ttgtgctggt
     1501 ggcaatcgcg gcagcgggat ggagcgaggc tgcgcgcgca caagacaaga agatcgcggc
     1561 agtcgatgaa gtgttcgtgg acgtggccaa gccgggttca ccgggctgcg cgctggcggt
     1621 ggcgcgcggc ggaaagatta tttatgaaaa gggctacggg ctggcgaaca tcgaagagaa
     1681 catacgcatt acgccgcaga gtgtgttcga catcgggtcg acttctaagc aatttactgc
     1741 ggcgagcatt ctgctattgg agaagcaggg aaaactctcg gtgaacgacg atatccggaa
     1801 gtatttgcca gagattccag actacggcca caagattacg attttgcact tgctgaacca
     1861 taccagcggg ctgcgcgact atttgacgct ttttgaactc gcggggatca atacggacag
     1921 cgtgacgacg gacgaggatg cgctggcgat cattgcgaac cagaaggcgc tgaattttga
     1981 accgggtagc gagtggctgt acagcaattc gggatttttt ctattgtcgg ggattgtgaa
     2041 gcgggtgagc gggaagacgc tgaccgagtt tgccgcggag aacatttttg cgccgctgga
     2101 gatgaagcat acggtttatc gggactcgca cacgctaatc gtgccgaatc gcgcgctggc
     2161 ttacgatgtg aaagagaagg aagagggata ttcgatcaac gtttcgtact tcgagcagac
     2221 gggcgacggt gcggtacaca cgtcggtgga agatcttttg aagtgggacg agaactttta
     2281 cagcggcaag gtgggcggca aggaatttct agtggagctg caggagccag gcaagctcaa
     2341 cagcgggaag ccgctgactt atgcgaaggg attgatggtg gaaaaatatc gcgggctgcc
     2401 gattgtgcag catggcggtt cgtggggagg ctaccgtgcg caattgctgc gattcccgga
     2461 acagcatttt tccgtggttt gcttgtgcaa tgtggggaac gccgggccag cgcagcgcgc
     2521 gcagcgcgtg gcggacattt atctagccga agaaatgaaa gagccggcag cgcgcgaaaa
     2581 agaaggaagg aagcgagaga agatagaaac ggttgccgtg gcggccgaaa aactagcagg
     2641 gtacgcgggg agttttcaca gcgaagaatt gcgcgcgact taccggctgg tggtggaaga
     2701 cggcaagttg aagctggcag gcattcagag tggcgacgga tttttgcatt cgccgcagca
     2761 tgtggaattg cggccggtcg ggccggacct cttcgtggtg gaagaagagg ggttgcagtt
     2821 tgcgttcgag cacaatggag cgaacgtcag cgggtttcgg ctggacgcgg gccgaacgaa
     2881 agggatcggg tttcagagaa agtagcctct ctagtggcgt agctgggggc tgaaatgcct
     2941 gagccagaga gtggcggctc cggcgccaag caggatgccg gccaaaatca agccgatgat
     3001 gaccagcgtg agccactttc ttgaaccatc ggcagcagga gcatttgaag agccgggcaa
     3061 ttcgccgctt tccgccggct tcgccgcggc cgcgtcccgc gtctcctgag gaaacggcgc
     3121 agcggagaat tcagcagtac cgataaagct gtagcccctg cggggaatcg tctcgatata
     3181 agtcggcttt tccgtggact cgccgagcgc ctggcggagc ttgttcaccg tggtgttgac
     3241 gttggcgtcg taattgacgt gggtatcggc gggccaaaga gcgtgcttga gctgttcgcg
     3301 gctgacgact tcgccctgat tgtgaatgag gacaagcagc acctgataga ccttgccctg
     3361 caagcgcaat ttgctaccgc cacgatatac ctgttggcgc tgccgatcga tctgaaaggg
     3421 gccaaaccgc acgtagcgcg gtagggaagc aatttcctgt agcgactcag tatccatgag
     3481 agggtccacg gagggaatga gattcgcagt catacacacg cccttgataa gggtagacgt
     3541 agaggacaca aaatcgttta aatctgatat acggaacagg aacgaaaatt gtttccggtc
     3601 aatctcgaaa tggcaaagaa aggcgcgaag aaccgggagt ctacgtaaga tgggatgaag
     3661 tttgaaaaac cgtagaaacg gcgaacggtg gaggcgcaaa gttctgaaac gtatgtaatt
     3721 ccacctgtga tttccttcct ttgagatgct ggtcacccga caaccatccg ttcgctaagc
     3781 catttaagtc cttagtcatg ccagagacaa aattgaggga caaattggcc tcgctggccg
     3841 cagaggtgcc aatccttcgt caaccaacct gtgctggcgg agtagtgcgg gggagtaagt
     3901 cgtaagccac caaacgctgc atgccacagg ttaaatcctg aaggagagaa agcaaatgcg
     3961 cagactgaca gtcatggcac tggcagcaat gctgtgtgtg ttgctgagca gtgtgacccg
     4021 ggcacagaga ttggatggca cccttcgagt aaccgtcacg gatcacagcc aagcgaccat
     4081 tgaggaagcc aaggtgacgg ctcaaaacga agctacgggg gtggcgaccg caacgtcggc
     4141 ctccagcgcg gggacctatg tgtttcctaa cttgttggtg gggacgtata cagtgaccgt
     4201 ggaaaaagat ggcttcaaga aggcggtgga gaaaggaatc tcggtcggct cgaaccaggt
     4261 agcggaagcg aaggttgaac tcgaggtggg ctccgtatct gcagtggtcg aagtcgaagc
     4321 aggggccgaa ctggttaaga ctcaatcgtc ggacctggac acaacattcg gagggaaaat
     4381 cgcgagcgac ttgccgatca acacggtggg cgggtccgtt ctggaattcg cagtgtttgc
     4441 gcccggcacc accacgcaac aaggcggagt ggcgggttcg gggggctcta tcggaggaaa
     4501 ccggccgcga ttcaacggct tttcgattga cggcgtggac gacaaccgca ccgacgtaaa
     4561 cggcccagcg acgccggtga ttcaagattc agtggcggag ttcaccctga tcaccaacca
     4621 attcagtgcg gaatacggac attcggcggg cgggcagttc agcatcgtga ccaagtccgg
     4681 taccaacaat tttcacggac aagcgcatgg gtacaaccag aatcgcaact acaacgcgat
     4741 ggacaacctg caaaaagatc gcgggcacca ggatcgattc gactacaacc gggcgggcgg
     4801 agatttcggc ggaccgatta agagggacaa actattcttt tttggcgcgt acgaatacca
     4861 gaacaatgga ttggcgtcgt cgagcccgac ggttcaagta cccacacaga acggactggc
     4921 gcagattctg gcgcagaatc cggatgccgc ggtgcaagca gcgctggctc agttccccat
     4981 cgctccaacc aagaccggca cggtaacggt gaacgggcag cccatcgatg tcggcacctt
     5041 ccagtccatc gcgcccagct acttcaacca aaatgacctg aacgcgaaca ttgacgcgaa
     5101 cctgggcaaa aaccaaatcc gggcgcgct
//
LOCUS       DASTPM010000620         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_483600, whole genome shotgun sequence.
ACCESSION   DASTPM010000620 DASTPM010000000
VERSION     DASTPM010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483600"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFR42_10915"
     CDS             complement(<1..476)
                     /locus_tag="VFR42_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0369715.1"
                     /translation="MALGALPARPQEPGQQPPVIRTQVNLVNLFVTVRDKNKRIVTDL
                     KQEDFKVQEDSQPQQIAFFSKEVTLPITLAMLLDTSGSEQYMLGAIQDAGGQFMNRVL
                     RKGDEALVMSFDSDVDLLSDFTDDHGQLDRAIRKARINIRSGGSIGNNPGPIGSRQ"
     gene            669..1790
                     /locus_tag="VFR42_10920"
     CDS             669..1790
                     /locus_tag="VFR42_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0369716.1"
                     /translation="MKSLRKIATAALIIAPALSGYILAQSAGQPAPGAAASQRSSPQA
                     GQSPAPPPSEARAKISVNSNLVILPVTVKDRSGNLVPDLQKNEFRVFEDNIEQKIDIF
                     TAEAFPLSMIILLDNDLKEKDAQQVRKSLDAVVGGLSLDDEAFICRFDEFFHPGSGFT
                     TDHDLLLTELQRTKIDSEPQVAPPGGAIANAPSINGHSAIGDSPNTASTTMQIRGQST
                     KALDDAVYGAAQLLRDRNRNRRKLILLISDGANGGKKTNKMNYDSVVKTLLHDNIEVY
                     SVAVASAFLERKFSRLVDYAHDSGGEVYFAAKRETLEELYSRISEEARNQYTIAYVPR
                     GTDRSADMHSVEVRVKREGLTIKTRDKYYAGEIPGAARP"
     gene            1822..1965
                     /locus_tag="VFR42_10925"
     CDS             1822..1965
                     /locus_tag="VFR42_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369717.1"
                     /translation="MTPPLPARISQAPGSTPAATPAIRSVKQGVPMKKKGKKDDKGGK
                     KGK"
     gene            complement(2075..>3191)
                     /locus_tag="VFR42_10930"
     CDS             complement(2075..>3191)
                     /locus_tag="VFR42_10930"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0369718.1"
                     /translation="ERPHVFEFPATLMKEGGPEEQFHNQEERRLFYVALTRAEDRLTI
                     SSLTEKKGRVPPFIEDILMEPSIQRRDVLRLAPKPAADAGNGARAGAPVAATGSLFDA
                     PVERPKIFSRIAEWATAFLPAPAEPLELNPSAVDSYRKCPQKYLFAYLWSLQESAKAT
                     LTFGRVMHATIRRAMHELKKGNRLPFEEVQRMFETEWSVSGFEDEYQEGEYKKDGLEQ
                     LRAFHQGMTDELPEILELEKTFVLPVENNVVLKGRIDQINSLGGKDVEIVDYKTGKPK
                     KESELRKDLQLSIYALAAKEIFELNPARLVFHYLQDNSRQETPRTVKQLDEALEIVQE
                     VAADIRARSFSAKETFACRSCSYRPICPAHEEPLSPE"
BASE COUNT          675 a         1018 c          841 g          657 t
ORIGIN      
        1 atttgcctcg agccgattgg tcccgggttg tttccgatac tcccgcccga acgtatattg
       61 atgcgcgcct tgcggatcgc acggtccagc tgcccgtggt catccgtgaa atccgacaac
      121 agatcgacat ccgaatcaaa cgacatgacc agcgcttcgt cgcccttgcg cagcacccga
      181 ttcatgaact gaccgccggc atcctggatg gctcccagca tgtattgctc gctgcccgaa
      241 gtatcgagca gcatcgccag ggtaataggg agcgttacct ccttggaaaa gaaagcgatc
      301 tgctgcggtt ggctgtcctc ctggaccttg aagtcttcct gcttgaggtc cgtaacaata
      361 cgcttattct tgtcgcgcac cgtcacaaac aggttgacca agttcacctg cgtgcgaatc
      421 accggtggct gctgccccgg ttcctgcggc ctcgcaggca aagcgcccag cgccattccc
      481 agggctacgc agaccaacag aaccttcttt gcgaaacgca tgtataattt ccttgtcgag
      541 aatacccggt cagaagtgcc cgccgctagg tcgctcaccc cccgtaggat gcggaggagc
      601 gcggcgcggg atgcgtggct cggcccgccg gagctgctgg aatccgcaga aagtttggcc
      661 tgaggaccat gaaaagtcta cgaaaaatcg cgaccgcggc cttaatcatt gctcccgcct
      721 tgagtgggta cattttggcg caatccgccg gtcaacccgc cccaggcgca gctgccagcc
      781 agagatcgag tcctcaagcc ggtcagtctc cggctccccc gcccagcgag gcccgcgcca
      841 agatctctgt aaattccaac ttggtcattc tgcccgtcac cgttaaggac cgctccggga
      901 atcttgttcc cgacctccaa aagaacgaat ttcgcgtttt tgaagacaat atcgaacaga
      961 agatcgacat attcaccgcg gaagccttcc ctctttcgat gatcatcctt ctggacaatg
     1021 acctcaaaga gaaggacgcg cagcaggtgc gcaaaagcct cgatgccgta gtgggcggtt
     1081 tgagcctgga cgatgaagct ttcatctgcc gctttgatga attcttccat ccgggctccg
     1141 ggttcacgac cgatcacgac ctgctgctca ccgagctgca gcgcaccaaa atcgattccg
     1201 agccacaggt tgcgccgcca ggcggcgcca tcgcaaatgc cccctcgatt aacgggcact
     1261 ccgccatcgg cgactccccg aatacagctt ccaccacgat gcagattcgc ggccagtcta
     1321 ccaaggccct cgatgacgcc gtctacggcg ccgcgcaact tcttcgcgac cgtaaccgca
     1381 accgccgcaa actcatcctg ctcatctccg atggcgccaa cggcggcaaa aagaccaaca
     1441 agatgaatta cgacagcgtg gtcaagacgc tgctgcacga caacatcgag gtctacagcg
     1501 tcgccgtcgc cagcgcgttc ctggaacgca agttttctcg cctggtggac tatgctcacg
     1561 attccggcgg tgaagtttat ttcgcagcca agcgcgaaac tctagaggaa ctttactccc
     1621 gcatcagcga agaagcccgc aatcagtaca ccatcgctta tgtgcctcgc ggcaccgatc
     1681 gctcggccga catgcacagc gtcgaagtcc gcgtcaaacg cgaaggcctc accatcaaga
     1741 ctcgcgacaa gtactacgct ggcgaaatcc cgggggccgc ccgcccctag agtagccctc
     1801 cggcgtctgc acgaataccg cttgacacct ccgctgccgg ctcgtatatc tcaggctccg
     1861 ggcagcacac cggcggcaac gccggctatc cgcagtgtga aacaaggagt ccccatgaag
     1921 aaaaagggca agaaggacga caagggcggc aaaaagggca agtagaagac cctaaaagct
     1981 tggatcaggg cgctgcgatt tcgcggcgcc ctttttttgc tagccttcgc ggcgttgcca
     2041 accggggcgc gatgctctgc ttcagaatgc cacgctactc cggggacaaa ggttcttcgt
     2101 gggcgggaca gatcggccgg taggaacaac tgcggcatgc aaaggtttcc ttggcggaaa
     2161 aacttcgcgc gcggatatcc gccgccactt cctgcacgat ctccagcgcc tcgtccagtt
     2221 gcttcaccgt gcgcggcgtc tcctggcgag aattgtcctg cagatagtgg aataccaggc
     2281 gcgcgggatt gagctcgaaa atctccttcg ccgccagcgc gtagatgctt agctgcaagt
     2341 ccttgcggag ttcggattcc tttttgggtt ttccggtctt gtaatcgaca atttcgacgt
     2401 ctttgccgcc aagcgaattg atctgatcga ttcgtccctt cagcaccaca ttattttcca
     2461 ccggcaagac gaacgtcttc tccagttcca gaatctccgg caattcgtcg gtcatgcctt
     2521 gatggaatgc gcgaagctgc tccaggccgt ccttcttgta ttcgccttcc tgatactcat
     2581 cttcaaaacc cgacaccgac cattccgtct cgaacattcg ctgcacttcc tcgaacggca
     2641 ggcgattgcc tttcttcaat tcgtgcattg cccggcgaat cgtcgcgtgc atcacccgcc
     2701 cgaaagtaag agtcgccttc gcgctctctt gcaacgacca caaataggca aaaagatatt
     2761 tctgcggaca ttttcgataa ctgtcgaccg cggagggatt cagctccaaa ggttcggcag
     2821 gcgccggaag aaatgcggtt gcccactccg caattcgtga aaagattttc gggcgttcca
     2881 ctggcgcgtc aaacagcgac ccggttgccg ccactggcgc gcctgctcgt gcgccattcc
     2941 cggcatcagc cgcgggtttc ggagccagcc gcagaacgtc gcggcgctga atgctcggct
     3001 ccatcaagat atcttcgata aagggcggca cccgcccttt tttctcggta agcgaactga
     3061 tcgtcagccg atcttccgcg cgggtcagcg cgacgtaaaa caggcgccgc tcctcctgat
     3121 tgtggaattg ctcctccgga ccgccttctt tcatgagcgt cgcgggaaac tcgaaaacgt
     3181 gcggtcgctc c
//
LOCUS       DASTPM010000621         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_887101, whole genome shotgun sequence.
ACCESSION   DASTPM010000621 DASTPM010000000
VERSION     DASTPM010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887101"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1486)
                     /locus_tag="VFR42_10935"
     CDS             complement(215..1486)
                     /locus_tag="VFR42_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEU0369719.1"
                     /translation="MDASLQLRAEQLAGEFANQVRTADELTDLMRLMMKSGLERMLNT
                     ELDVHLGRKSAVVGGVPVAEIEPAEPRGKRASNRRNGHSPKTVQGDLGEVTLAVPRDR
                     NSTFQPQLIPKHQRRVPGFDEKILALYAKGMTTRDIQEIVQELYGVEVSATLVSEITA
                     DLDVEVTAWRTRPLAPVWPIVYFDGIVVHVRSASGKVAPHTVYVALGVNLAGHKELLG
                     LWLGENEGAKFWLSCLTDLKNRGLRDLFVACIDGLSGFADAIRAAYPQATVQLCLVHL
                     VRAALRYVSTEDSKPVVADLKKIYQAATVVEAEQALDAFAQAWDAKYPTIAKAWRSKW
                     ADIITLFDYPPAIRRVIYTTNAIESVNSVIRKFTRNRKIYPNEESALKIVYMAIREAS
                     RKWTMPIHHWKQALNHFAILFEGRMPDNLNS"
     gene            1846..>2055
                     /locus_tag="VFR42_10940"
     CDS             1846..>2055
                     /locus_tag="VFR42_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369720.1"
                     /translation="MLKYLVVLGLLTVLGKRRLGATIPWPLLLGGVTAFLSLGMSPYL
                     TSFARPETGVIIGLCVLCLLTIYMQR"
BASE COUNT          397 a          628 c          574 g          456 t
ORIGIN      
        1 tggctgaatt taacgagcag atgcgacggc ggtattgtcc tggctgtggc gagggctgag
       61 cgggcatgag gccgtggctg ccagggcaga agccagcttt ggaagactcg gaaaatcact
      121 ctctcggtca gcacgatttc gagactattg atccgaaccc tcgacaccgg ctcagcgggc
      181 ctgtaaattc ttttgtgtca gccgagcagt ttaatcacga attcaggttg tcgggcattc
      241 gtccctcgaa gaggatggcg aagtggttga gggcttgctt ccagtgatgg atcggcatgg
      301 tccactttcg cgacgcctca cgaatcgcca tgtagacgat cttcagggcc gactcttcgt
      361 tgggataaat tttgcggttg cgggtgaact tacggatcac gctgttcacc gactcgatgg
      421 cgttggtcgt gtagatcact cgccgaatcg ctggcggata gtcgaacagc gtgatgatgt
      481 cggcccactt gctccgccag gccttggcaa tcgtcggata cttggcgtcc caggcttgag
      541 caaaagcgtc gagcgcttgc tccgcttcca ccaccgtggc ggcctgatag atcttcttca
      601 gatcggccac caccggtttg ctatcttccg tgctcacgta gcgcagcgct gcccgcacca
      661 agtgcacgag gcacagctgc acggtggcct gcggataggc ggcgcgaatg gcgtcggcaa
      721 agcccgagag cccatcaatg caggccacga acaggtcgcg caagccgcga ttcttcaaat
      781 ccgtcaggca cgacagccag aacttggccc cttcgttctc ccccagccac aagcccagca
      841 gttctttgtg acccgctaaa ttcacgccta acgccacgta gaccgtgtgc ggcgcgactt
      901 tgccgctcgc cgagcggaca tgcaccacga tgccgtcgaa gtacacaatc ggccagactg
      961 gcgccaacgg ccgcgtgcgc caagcggtca cttccacatc cagatcggcc gtgatctcgg
     1021 acaccaacgt cgccgagact tccacgccgt acagttcctg gacgatctct tgaatatcgc
     1081 gagtggtcat ccccttggcg tacagcgcca gaatcttctc gtcgaagccc ggcacccgac
     1141 gttggtgctt gggaattagt tgcggttgga aggtgctgtt gcggtcccgg ggtacggcga
     1201 gcgtcacctc tccgagatcc ccttgcacgg tcttgggaga atgcccattt cgccggttcg
     1261 aagcccgctt gccacgcggc tcggccggct caatctccgc cacgggcact ccgcctacca
     1321 ccgccgattt gcgccccagg tggacgtcca actcggtgtt cagcatccgc tccaaacccg
     1381 acttcatcat cagccgcatc agatccgtca gctcgtcggc cgtccgcact tgattggcaa
     1441 actcccccgc caactgctca gcccgcaact gcaatgatgc atccatgcgt caattctcca
     1501 gatcagaaaa aggtaattat aactaccagc ttctgctagc tgacacagga tttcttacag
     1561 ggccgccgct tgcatgggaa gcgcctgctc tgggtgttga cggtcggcag tctcctgtct
     1621 ttcatattga cctttgtcac gctaacgagc gcgccactct tcttcgatga agcccattgg
     1681 cagatacgga acgcgagagt gccacgcgat ggtgggaaaa tggtaaacct acacatcgcg
     1741 tgcgaatcga acttgagctg gaccgttccg ccgcttctct tgcctggcag atactttgcg
     1801 gctcaatttc acccagcatc accccaaatg ctgaggcaat tcggtatgtt gaagtatttg
     1861 gtggttctag ggctattaac cgtgttgggc aagcggcgcc tgggcgcgac cattccctgg
     1921 ccgttgttgc tcggcggcgt gactgccttc ctaagcttgg gtatgtctcc ctatctgacg
     1981 agctttgcgc gaccggaaac aggcgtcatt attggcctat gcgtgttgtg cctgctcacg
     2041 atctacatgc agcgt
//
LOCUS       DASTPM010000622         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_79700, whole genome shotgun sequence.
ACCESSION   DASTPM010000622 DASTPM010000000
VERSION     DASTPM010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79700"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFR42_10945"
     CDS             complement(<1..804)
                     /locus_tag="VFR42_10945"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0369721.1"
                     /translation="MELRVKRAPRIDIEITVPGDKSISHRAVIISALSNGVCTLQGFL
                     PSEDCMHTVNALRALGIKIEQPEPATLIVHGKKRVLTPPKNEINCGNSGTTMRLLAGL
                     LAGQTFESRLVADAGLSKRPMERVIAPLRQMGANIVAEGPEDTPPLRIQGGSLRGIRY
                     RPPVASAQAKGALLLAGLFAKGKTTVIEPIPTRNHLEMTFDYFLVRIAVDGNGSVTVF
                     GDQVPESRDFSIPGDISSAAFWLVAAAAQRRGHLLIRDVGLNHTRTALLG"
     gene            1426..2640
                     /locus_tag="VFR42_10950"
     CDS             1426..2640
                     /locus_tag="VFR42_10950"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU0369722.1"
                     /translation="MAVRLPSDSKLEMSTSNNSHAEILIVDDDALSRKILAQVLTSNG
                     YNCQVCGDGAEALEIIHAKPPSLLLLDFDMPGANGAEVLKQLRSDQYQAVAQVPTIML
                     TGHGSEESEVSCLQAGADDFVTKPVNAAVLRARIETQLRLRSMRRQLERQNDELEQWR
                     RDLERDLAAARLTQQSLIPQKPLAVPGWEVAACYRPVIQVGGDIYGWLRMKDGRVLFW
                     IADGSGHGAAAALLTTLAKLLFHHGSVEHDTPASVMEAVNDDFRSIFGGRSFMTAMCV
                     ALNHTTGSAHIVGAGHPPLLIARHNGATESIASLTPPLGLAKRPEFVETALDFKPGDA
                     FLLYTDGLFGSSKNEQRRLTPQQLEKMLDHSAPTAEAFLKRILAQATPNSTENSLSDD
                     LTALAVRRMAPE"
BASE COUNT          654 a          785 c          777 g          597 t
ORIGIN      
        1 acccagtaac gccgttcgcg tgtggttcaa gccaacatcg cgaatgagca gatgacctcg
       61 tcgttgcgcc gcagcggcaa ccagccaaaa ggccgcggaa gagatgtcgc ctggaatgga
      121 aaagtcgcgg gactctggga cctggtcgcc aaacacagtg acgctgccat ttccgtccac
      181 cgctattcgg accagaaaat aatcaaacgt catttccaga tgattccgag ttggtattgg
      241 ctcgattacg gtggtcttgc ccttggcaaa gaggccggcc agcagcagtg cacctttcgc
      301 ctgtgcgctt gcgacaggcg gtcgataacg aatgccacgc aaagaaccgc cctggatccg
      361 cagcggtggc gtatcttctg gaccttccgc gacaatattt gcacccatct gacgaagcgg
      421 cgcaatgacg cgctccatcg gacgttttga caggccggca tccgcgacaa ggcgactttc
      481 aaatgtctgc ccagcaagta acccggcgag aaggcgcatt gtggtgccgg aattgccgca
      541 attgatttcg tttttgggcg gcgtaaggac ccgctttttc ccatgcacga tcaaggtggc
      601 cggctcaggc tgttcgatct taatcccgag tgcgcgcagc gcattcacgg tgtgcataca
      661 atcttcgctg ggaagaaacc cctgcagagt gcacactcca ttcgacaagg cagagatgat
      721 taccgcgcga tgggagatac ttttatcgcc cggcacggta atctcgatgt caattcgagg
      781 tgcccgtttt acccgcaact ccatagataa gaagaaaccg ttggtgaata ctcaatacgg
      841 gatgctcgat aaaagacagg cgccgcgcta attcggcacc cgataccagc acccagcatc
      901 agcctcgaac accctcaagg caacccgtta cggaacaaga aaaatcttat ccgtgtacgg
      961 atcctttacc tccgttcccg gagggaaacc ctccacatcg atatatttgc caggtgaata
     1021 aggactctct accaggtgag gcttgccggg aacgggaatt ccgtacggat aatccccctt
     1081 tgccaccttc gtcggcgcgg ctgggggagg aggtggcgct gctgcggttt ccacgggcgg
     1141 tgtacctgta ggttgaactg ggccattcgg attgaatggc tgttgcggtg gcgggtactg
     1201 gtcgggctgg gtaggggcat agctcgcggc ccgatgtcga gagggtggtg gcggctcttc
     1261 actgcatgag gcaacgagga aggccgtgaa cagagcaagc gacaatgaaa atttcataaa
     1321 tggaattact gtcacaatcg tatccgcagt ccgagtcgtt gccaatcatt aaatcgcttt
     1381 ttgttcacac tcggagatgc tttcgcaaaa attggtgcgg atacaatggc ggtccgcctt
     1441 ccatccgatt ccaagttaga gatgtcgacg tccaacaact cccacgcaga aatcttgata
     1501 gtggatgatg acgctttgag ccgcaaaatc ctcgcgcaag tgctaacgtc caacggctac
     1561 aattgccagg tgtgcggaga cggcgctgaa gccctggaaa taatccacgc caagccgcct
     1621 tcgcttctgt tgctcgactt cgatatgcca ggggcaaatg gcgccgaggt gctaaaacag
     1681 cttcgctcgg atcaatacca ggcagtcgca caggtgccga caattatgct aaccgggcac
     1741 ggaagcgagg aaagcgaagt ctcttgtttg caagctggcg cagatgattt tgtgacaaaa
     1801 ccagtgaatg ccgcagtgtt gcgtgcgaga atcgagaccc agcttcgtct ccgctccatg
     1861 aggcggcaat tagagaggca aaatgacgaa ctcgaacaat ggcgccgtga cctcgagcgc
     1921 gatctggcgg cagcgcgttt gacgcagcaa tcgttaattc cgcaaaagcc tctggcggtg
     1981 cccggttggg aggttgccgc ctgttaccgc ccggtgatcc aggttggggg cgatatctac
     2041 ggttggctgc gaatgaaaga tggccgagtc ttgttctgga ttgccgacgg ctccggtcac
     2101 ggggccgctg ctgcgctgct taccaccctg gcaaagcttc tctttcacca tggcagtgtg
     2161 gaacacgaca cgccagccag cgtgatggaa gcagtcaacg atgactttcg cagcattttt
     2221 ggcgggcgct ctttcatgac agccatgtgt gtcgcgctca atcacacaac cgggagcgca
     2281 cacatcgtgg gagccggtca tccgccgctt ctcatcgccc gccacaatgg tgcaacggaa
     2341 tcgattgcat cgctcacacc tccgttgggc ttggccaaac gccccgaatt tgtggaaacc
     2401 gcgctcgact ttaaaccagg ggacgccttc cttctctata ccgatggctt gtttggctcg
     2461 tcgaaaaatg aacagcgtcg gttgacgcct cagcaattgg aaaaaatgct ggatcactcc
     2521 gcgccgactg cagaagcgtt cctcaagaga attttggcgc aggcaactcc aaatagcacc
     2581 gaaaactcct tgtccgatga tctgacagct ctggcagtgc gccggatggc cccggaataa
     2641 atttttcgtt tgcctaaaat tagtgttgct ctctctcaag cgaaacgtta atcagcctcc
     2701 atgccgaaaa ccaaaaagaa aacttccaag cgcaaaccga atcccgcctt catgagaccg
     2761 gtccagcctg acgataagct tgctgccatt gtcgggtcgc agccgctacc acg
//
LOCUS       DASTPM010000623         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_570388, whole genome shotgun sequence.
ACCESSION   DASTPM010000623 DASTPM010000000
VERSION     DASTPM010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570388"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VFR42_10955"
     CDS             <1..383
                     /locus_tag="VFR42_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU0369723.1"
                     /translation="LHPQVKLRTNSVSTSEQTAASRLEGDSPGHIVDPASGTPLRTGY
                     AVSVTAKSATASDALSTTLLLLGPEKGVALVAGLKDVSAIWVSADGRRTIAGSKTAIF
                     VHPDSGPAATAATVKSAGQRENEN"
     gene            386..1825
                     /locus_tag="VFR42_10960"
     CDS             386..1825
                     /locus_tag="VFR42_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369724.1"
                     /translation="MLLHAYAYLLLVAVGLSVEPAAGNVLLRVAPGPLPPAKAIGVSD
                     IVLRWPAGETADLTKLRALGFHLFLEAGSSDLAAASAAAERADAAGVIFDHSGSDSAA
                     EGELFRSSKMAHPTLLFRFLISGGKQPQMRGRLVVDRDGILQVSSPSSQPWLDTNLAR
                     TRLAETFHPDALPVLYDFQWDLADSLHKQLGPSVDEYALAIAEAEAIRADVILDFPEP
                     LQTALAADQADAWALWNQVKRYIAFSSPVSSERIQSIANMGVVTCDDQMNYEAVNLLA
                     RHNLAFQTVRPQDLSAERLNAWNAVVVFCSLRSAEIDLLNVFVKRGAIVVLVNQRGEY
                     PWHSAAPVRQDAHSATFAVGSGQLIELREPVTDPELFARDLRRLIGTERSAVGLWNSM
                     TTLVTGYRQESGGETILNVVNYADVPENVQVQIKGQFASIQLESPEELCCRALQVFTR
                     GGFTEFTIPRLVIAARVHLKSADGSGANP"
     gene            1918..3276
                     /locus_tag="VFR42_10965"
     CDS             1918..3276
                     /locus_tag="VFR42_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0369725.1"
                     /translation="MPSFCRIYLHAALIAATLLALALPSAAQSPSYDLLLKGGHIIDP
                     ANNRDAVLDVAVAGGKIAAIGKEISAAQAKKVVDVTGLYVTPGLIDIHYHIGHGGAPL
                     DWFTPQARVHLQPLGILADWALTSGVTTIVDAGTAGADTFLLEKEEVIDRAKVRVLAF
                     LNIVANGMEGGLEQSVDQMDPKVCAETLKKFSDVIVGVKTAHYWTTGPWDGEHTPWAA
                     VDRAIACAESAKVPVMYDFWPRPDRSYSDLILKKARPGDIHTHVFAQQFPILLQDGKL
                     NPILTEARQRGVFFDVGHGAGSFWFRNAVPAAKQGFLPDSLSTDLHTGDYRTVSMANV
                     ISKFRAMGVPLADLIRRSTVNPAREIHREDLGTLSVGKEADIAVLEELHGKFGYIDCG
                     LAKMNGDVQVIARMTVRAGRVAYDPSGMSMVEWEKARPQYFNNPSEGNSAPATAEDSP
                     ER"
     gene            3304..>3978
                     /locus_tag="VFR42_10970"
     CDS             3304..>3978
                     /locus_tag="VFR42_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369726.1"
                     /translation="MSAKMSRRKLLRQGSEAVVAGSVGACATMITGSIPLAVAAGATP
                     VPAKGVDYYEKLGLVPFINAAGTYTILSASTMPDEVQAAIALAAKRPVHLLELHEAAG
                     TYLAQKLRCGGALVTSGASAGLTLGTAACMTLGNKEAIVSIPTDVSGLKNEVIVQKVH
                     RYEYDHAMRNCGAKVVEVETLEQYQNAFSDKTVMTNFFNAVEAPIGREDWVRVAHQHG
                     VPCFNDA"
BASE COUNT          825 a         1170 c         1132 g          851 t
ORIGIN      
        1 ggctgcatcc gcaagtgaag ctgaggacca atagtgtttc gacgtccgag cagacagccg
       61 cgagcaggct tgaaggcgac tcccccggac atatcgtcga tcccgccagc ggtacgcctt
      121 tgcgcaccgg gtacgccgtc agtgtgaccg caaaatcagc gacggcctcc gatgcgctct
      181 caaccacact gcttctgctc ggccccgaga agggcgtggc gctggtcgcg ggccttaaag
      241 atgtcagtgc gatatgggtt tccgccgacg gtcgtaggac aattgcgggc tcgaaaacgg
      301 ccatcttcgt acacccggac tctggcccgg ccgcaaccgc agcgaccgta aagagcgctg
      361 gccaacggga gaacgagaac tagctatgct tcttcatgcc tacgcttatt tattgctggt
      421 ggcggttggg cttagtgttg agcctgcggc gggaaatgtt ctcttgcgcg ttgcgcctgg
      481 cccgctgcct cccgccaaag ccatcggagt cagtgatatc gtcttgcgtt ggccggctgg
      541 tgagactgcc gacctgacga aattgcgggc gctcggcttt cacttatttc ttgaggcagg
      601 ttcaagcgac cttgcggccg catccgcagc tgcggaacgc gccgatgccg cgggcgttat
      661 cttcgaccat agcggttccg acagcgccgc cgaaggcgaa ctttttcgat cgtccaagat
      721 ggctcatccg accctgcttt tccgtttctt gatctccggc ggcaagcaac cgcagatgag
      781 agggcgcctg gtcgtggacc gtgacggaat tctgcaggtt tccagccctt cttctcaacc
      841 ttggctggat accaacctgg cccgcacgag gttggcggaa acatttcacc cggacgcgct
      901 acccgttctc tacgattttc agtgggatct cgccgattcc cttcacaaac agctgggacc
      961 aagtgtcgac gagtatgccc tggcgattgc cgaagccgaa gcaattcgtg ctgacgtaat
     1021 tttggatttt cccgagccgc tgcagacagc gctggctgcc gatcaggccg acgcatgggc
     1081 tctttggaac caggtgaagc gatacatcgc gttttcctct cctgtttcgt cggagagaat
     1141 acaatccatc gcaaacatgg gagtagtcac ttgcgacgac cagatgaact acgaagcagt
     1201 gaatctgctg gctcgacaca atcttgcatt ccagacagtt cgcccgcagg atctgagcgc
     1261 ggaacgattg aacgcgtgga atgcggtggt ggttttttgt tcgttgcgga gcgcggaaat
     1321 cgatttattg aacgtctttg tcaaacgcgg cgcaatcgtc gttctggtca atcaacgcgg
     1381 cgaatatccc tggcattcgg ccgcgccagt ccgtcaggac gcgcactcgg caactttcgc
     1441 ggtcggatcc ggacaactca tcgagctcag ggaaccggtg accgatcccg aactgtttgc
     1501 gcgcgacctg cggcgcctta tcgggacgga acgttccgca gttggcttgt ggaattccat
     1561 gacgaccctg gtgaccggat accggcagga atcgggaggc gaaacgattc taaatgtggt
     1621 taactacgcc gacgtacctg aaaacgtaca agttcaaatc aaggggcaat ttgcttcgat
     1681 tcaactggaa tccccggaag aactttgctg ccgcgccctg caagttttta cacgcggcgg
     1741 tttcactgaa tttaccatcc ctcgtctggt gatagcagcc cgggtgcact tgaaatctgc
     1801 tgacggcagc ggcgcaaatc cctaaagact gcgtgggatt ctgagacttt gctggacgcg
     1861 tcgttggtgg acgatagtac ttgacaagaa aaagcgaaga agggacggag acccaacatg
     1921 ccctcgtttt gccgaatcta tttgcacgct gcactgatcg ccgccacgct cctcgcgctc
     1981 gctttgccct cggcggccca gtctccatcg tacgacctcc tgctgaaagg agggcacatc
     2041 attgaccccg ccaacaaccg ggacgcggtt ctggacgtcg ccgtcgctgg cggcaagatc
     2101 gctgcgatag gcaaggagat ttctgccgcc caggcgaaga aggtggtcga tgtgaccggg
     2161 ctctacgtta cacccggctt gattgacatt cactaccaca tcggtcacgg tggagcgcca
     2221 ctcgattggt tcacgccgca agcgcgcgtg catctccagc ctctcggtat tctggccgat
     2281 tgggctttga cttccggcgt caccacgatt gtcgatgcgg gtacggcagg tgccgacacc
     2341 ttcctcctcg aaaaagagga agtcatcgat cgcgcaaagg ttcgagtctt ggcttttctc
     2401 aacatcgtcg caaacggaat ggagggaggg ctcgaacaat ctgtggacca gatggatccc
     2461 aaggtctgcg cggaaacgct taagaaattt tccgatgtga tcgttggtgt caaaaccgcg
     2521 cactactgga ccacaggtcc gtgggatggc gaacacactc cgtgggcggc ggttgatcgc
     2581 gccatcgcat gcgccgaatc agccaaggtt cccgtgatgt atgacttttg gccccggccg
     2641 gatcgttctt attctgacct catcctgaaa aaggcgcgtc ccggcgacat ccatacccat
     2701 gtttttgcgc agcaatttcc gatcttgctc caagacggca agctcaatcc catcttgacc
     2761 gaagcgcggc agcgtggcgt gttctttgac gtaggccacg gtgcaggaag cttctggttc
     2821 cgcaacgccg ttcccgctgc gaaacaaggc ttcctgcccg actcgctttc cacggatctc
     2881 cacaccggcg attaccggac cgtcagtatg gcgaatgtga tttccaagtt tcgcgccatg
     2941 ggtgttcctc tcgccgatct cattcgccgc tccacggtca atcctgcccg tgaaattcac
     3001 cgtgaggatc tcggtacact gtctgtcggc aaggaggccg acatcgccgt gctcgaagag
     3061 ctgcacggaa agtttggtta catcgactgc ggtctggcca agatgaacgg cgatgttcaa
     3121 gtgattgcgc gcatgaccgt tcgcgccggc cgggtcgctt atgatccctc cggcatgagc
     3181 atggtcgaat gggaaaaggc ccgcccgcaa tatttcaaca atccttcgga aggcaattct
     3241 gctccggcta ccgccgagga ttcgcccgaa cgctgattac gctacgccaa ggaggagaat
     3301 ctgatgtccg caaaaatgag tcgccgcaaa ttgctccgcc aggggtcgga agccgtcgtt
     3361 gcaggaagtg ttggtgcttg tgcgaccatg atcaccggtt cgatccccct ggcagttgcc
     3421 gcgggcgcca ctccggttcc ggcaaagggc gtggactact acgagaagct tgggctggtc
     3481 ccgtttatca acgccgctgg aacctacacc attctgagcg cctccaccat gcccgatgaa
     3541 gttcaggccg ccattgcgct ggctgccaaa aggccagttc atcttctgga actccatgaa
     3601 gccgccggaa cctatctggc ccaaaaactt cgctgtggcg gagcgctggt aacctccggg
     3661 gcatcggctg gactcaccct cggaaccgct gcctgcatga ccctgggcaa caaagaagcc
     3721 atcgtcagta ttcccaccga tgtaagcggg ctgaaaaacg aagtcatcgt ccagaaagta
     3781 catcgctatg aatacgacca cgcgatgcgc aattgcggag caaaagtggt cgaagtcgaa
     3841 actctcgagc agtatcaaaa tgccttttcg gacaaaacgg taatgaccaa cttcttcaac
     3901 gccgtggaag cgccgatcgg ccgcgaagac tgggtccggg tcgctcatca gcacggcgtt
     3961 ccttgcttca acgatgcg
//
LOCUS       DASTPM010000624         5282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1233280, whole genome shotgun sequence.
ACCESSION   DASTPM010000624 DASTPM010000000
VERSION     DASTPM010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233280"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFR42_10975"
     CDS             <1..228
                     /locus_tag="VFR42_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369727.1"
                     /translation="DEANVGRFTRLIGEMSAQTQFIVVTHNRKTMETGSVLYGVTMQE
                     PGVSKIVSVRWEGEESPAPGHRKSAAAATAA"
     gene            526..1407
                     /locus_tag="VFR42_10980"
     CDS             526..1407
                     /locus_tag="VFR42_10980"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU0369728.1"
                     /translation="MKALQETNFPGLKPSARGKVRDIYDLGDQLLMVATDRLSAFDVV
                     MPTPIPDKGKVLTQISLFWFDLLKDVVRNHVLRADNFPAPFDRCGDQVAGRSMLVRKT
                     RPLPIECVVRGYLSGSGWKEYSATRKVCGIALPAGLRESDKLPEPIFTPATKATSGHD
                     ENISFDHAAEVVGRPLAERVRAVSLEIYNRAAAFAAPRGVILADTKFEFGQLNNELIW
                     IDEALTPDSSRFWPASGYSPGGPQPSFDKQFVRDYLERIRWPKTPPGPDLPPDVVAAT
                     RAKYREAFRLLTGRELT"
     gene            1453..1674
                     /locus_tag="VFR42_10985"
     CDS             1453..1674
                     /locus_tag="VFR42_10985"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0369729.1"
                     /translation="MKDQLEGLVTQMVERGILFDEAVGEFEKRFIKRILDRAEGNQCR
                     AAKMLGIHRNTLSRKIGEYKLDHSVARKR"
     gene            complement(1745..3082)
                     /locus_tag="VFR42_10990"
     CDS             complement(1745..3082)
                     /locus_tag="VFR42_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369730.1"
                     /translation="MRRIAGLAVSASLCWMLGSAASAQAPAGGQDAGKQPYTMPEYNA
                     EQACGNDKNPSTQVKCLDDFVSKYPNSNLLIYVYPMYYQSYFQLKNYAKVMEYADKMV
                     ALGDKVDANAKYAALYARALAFASLPAAEQATQAKGAVTAAEAALKLIPELKKPDNMD
                     DNAFSEQKKQATSYLNGTAAQAAMVSKDYPNAIKYYKAVLADQPKPEVEAITEYRLGQ
                     AYLGSTPPQQIDGFWHVARAITSKGATQQQVTQLKTYLRKLMVNYQQAACDNLIDAEF
                     NELLQLAGSSADRPDTYKIPSATDLDAARKDMTIASVITDLKAGGDKAKVTWLASCGL
                     EFPDVPGKLMEITPGATAADPIVLQVAFVTSDAEFQAATKPNMEVKVVGQPEAAKLEK
                     DNPVRFSGTVMSYDPDPAFMLHWDKAKVNAEDIPADKTPAKKPPVRRPAPKKP"
     gene            3478..4221
                     /locus_tag="VFR42_10995"
     CDS             3478..4221
                     /locus_tag="VFR42_10995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0369731.1"
                     /translation="MDSIHETNPPSAKPLAGKVALVTGGNRGIGKAIALRLASLGSAV
                     GICGRDTSTLASTTIELGTFDVRVHSQPADVTRVYDVSALVQQTEAALGPISILVNNA
                     GMGLFGPVHEKSETEWDTLLDTNLKSVFLVSRAVIPGMIQRRSGDIINISSLAGKNTF
                     AGGGIYCASKWALQGLSGCMAEDLRTYGIRVSAVCPGSVFTEFSGRGAKDPSKVLAAD
                     DVAHAVAMIATQGPQSFLSEVHLRPVSKP"
     gene            4376..>5282
                     /locus_tag="VFR42_11000"
     CDS             4376..>5282
                     /locus_tag="VFR42_11000"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0369732.1"
                     /translation="MRLQFLFPFALLAIAAPSRAADSPAPLRVAIAGLVHGHAEGFFS
                     HSLSRNDIQIVGIAEPDHALFDRYAAKYKLDASLYHADLDELLRSTHPQAVLGYTSTL
                     DHRKIIETCARYSVPVMVEKPLATTYEDAKAIAAAARGAKIHVLVNYETSWYASNRAA
                     YELLHSGAIGDLRKVVVHDGHEGPKEINVGPEFLGWLTDPKLNGGGALFDFGCYGADL
                     MTYLMDGARPLTVTAVTQRMKPDIYPRVDDEATIVLTYPRAQAILQASWNWPYSRKDM
                     EVYGRTGSVITIANNDINVRLPHERQ"
BASE COUNT         1012 a         1803 c         1324 g         1143 t
ORIGIN      
        1 gacgaagcca acgtcggccg cttcactcgc ctgatcggcg aaatgagcgc gcaaactcaa
       61 ttcatcgtcg tcacgcacaa tcgcaaaacc atggaaaccg gcagcgttct ctacggcgtc
      121 accatgcagg agcccggcgt ctccaaaatc gtcagcgtcc gttgggaagg cgaagaatct
      181 cccgcgcccg ggcaccgcaa atccgccgca gccgccaccg ccgcctaact cttgccaaga
      241 tttgtcgtcc tgaggatctc agcgtccgcc gccattactt gagatcttcg cgacgaccgt
      301 actccacggc cgcgctcgtc attccgaacc tgcccgcctc ggcaggcgct gcgaggttcg
      361 gttttgcttc gagcagtttt tgcccgatgc atctctcttc aatcgctcgt cctcccggac
      421 ttttccaaca aaaatccacc gtcgttctct ttgacttccc gcctcgctgc gctaaaatcc
      481 tcgctcttcg ttcaggggaa gcgtttggtg tcgttggcgc cagcaatgaa agcattgcag
      541 gaaacaaatt ttccggggct caagccctcc gctcgcggga aggttcgcga catctacgac
      601 ctcggcgacc agctcctcat ggtcgccacc gatcgtctct ctgcattcga cgtcgttatg
      661 cccacgccca tcccagacaa gggcaaagtc cttacgcaaa tttccctttt ctggtttgat
      721 ctgctgaagg acgttgtcag aaatcacgtc ctgcgcgcgg acaacttccc cgccccgttc
      781 gataggtgcg gcgatcaagt cgccggccgc tccatgctgg ttcgcaaaac tcggccgctc
      841 cccatcgaat gcgtggtccg cggctatctc agcggctccg gctggaaaga gtactcggcc
      901 accagaaaag tctgtggcat cgctctgccc gctggcctgc gcgaatccga caagctcccg
      961 gagccaatct tcactccggc caccaaggcg acgtctggcc acgacgaaaa tatttcgttt
     1021 gaccacgccg ccgaggttgt cggccgtcct ctcgccgaaa gagttcgcgc cgtcagtctc
     1081 gaaatctaca atcgcgctgc cgccttcgct gccccgcgcg gcgtcatcct ggccgacacc
     1141 aaattcgaat tcggccaact caataacgaa ctcatttgga tcgacgaagc cctcacgccg
     1201 gactcatccc gtttctggcc tgcctccggg tactcgcccg gcggccccca accctccttc
     1261 gacaaacagt ttgtccgcga ctatctggaa aggatccggt ggcccaagac tccgccgggt
     1321 cccgatttgc cccccgacgt agtcgctgca acgcgcgcca agtaccggga ggcttttcgc
     1381 ctcctaactg gccgcgaatt gacctagcaa gccggccctc aagaaaaact ctcaccgcgc
     1441 ggagcgtaca tcgtgaagga ccaattggaa gggcttgtaa cacaaatggt ggagcgggga
     1501 atactcttcg atgaagccgt cggcgaattc gaaaaacgct tcatcaagcg catcctggat
     1561 cgcgccgaag gcaaccagtg ccgcgccgcc aaaatgctgg gcatccatcg caacaccctc
     1621 agccgtaaaa tcggcgaata caaactcgac cacagcgtcg cccgcaagcg ctaggcgatt
     1681 ctcgcttttg tagcggccgg cttctcaggc ggacgcctct ggctcgcaaa tttgctcaaa
     1741 gttcttacgg cttcttcggc gcgggccttc tcaccggcgg cttcttcgct ggcgtcttat
     1801 ctgccgggat gtcctccgcg ttcaccttgg ctttgtccca gtgcagcatg aacgccgggt
     1861 ccgggtcata cgacatcacc gtgcccgaga acctcacggg attgtctttt tccagtttcg
     1921 ccgcttccgg ctggcccaca actttcactt ccatgttcgg tttcgtcgcc gcttggaact
     1981 ccgcatcgct cgtcacaaac gccacctgca acacgatggg atcggccgct gtcgcccccg
     2041 gtgtaatttc catcagcttg cccggcacgt cgggaaattc cagcccgcac gacgccagcc
     2101 acgtcacctt ggccttgtcg ccacccgctt tcaggtccgt gatgaccgac gcgatcgtca
     2161 tgtccttgcg cgccgcatcc aaatccgtcg cgctggggat cttgtacgta tccggccggt
     2221 cggcggagct tcccgccaat tgcagcagct cgttaaactc ggcgtcaatc aggttgtcac
     2281 acgccgcttg ctgataattc accatcagct tgcgcaaata ggtcttgagc tgcgtcactt
     2341 gctgctgtgt cgcgcccttt gacgttatcg cccgtgctac gtgccagaag ccgtcaatct
     2401 gctgcggcgg cgtcgagccc aaataggcct ggcccaaccg gtattccgtg atcgcctcca
     2461 cctcgggctt gggctggtcg gccagcaccg ccttgtaata cttgatcgcg ttcggatagt
     2521 ccttgctgac catcgccgcc tgggccgccg tcccgttcag gtaactcgtg gcttgcttct
     2581 tctgttcgct gaaggcgtta tcgtccatgt tatccggctt cttcaactcc ggaatcaact
     2641 tcagcgctgc ttccgctgcg gtcaccgctc ctttcgcctg cgtcgcctgt tcggctgcgg
     2701 gcaggctcgc gaacgccagc gcccgtgcgt acaaggccgc atactttgca ttggcgtcca
     2761 ctttgtctcc caacgccacc attttgtccg cgtattccat caccttcgcg tagtttttca
     2821 actggaagta cgactggtag tacatcgggt aaacatagat cagcaaattc gaattgggat
     2881 acttggagac gaagtcgtcc aggcacttca cctgcgtgct gggattcttg tcgttgccgc
     2941 aagcctgctc cgcgttgtac tcgggcatgg tgtagggctg cttgccggca tcctgtccgc
     3001 ccgccggcgc ctgcgccgac gcagcgctac ccagcatcca acacaacgac gcggacaccg
     3061 ccagccccgc aattcgtctc attcggttca ttcccattct cctcactcaa acttcaaact
     3121 ttgaaatttg ttccggccaa gcaagttttc cgggaactcc gcaaataatg gcgcgggcaa
     3181 aagatttgcc cggtacgaaa tccttgggat tataggtaaa cgcatacacc caccgcaagt
     3241 tgttcccata caccgaaatc tgcacgctgc ggaaattctt cgcgtaatag acgcactgtt
     3301 tttccttagt agcgtgcctt tctgcacatc acgctgcgcc tttgtctcag tattcatctc
     3361 accggtgagt ttttgcacct ggccggcatc gtcctgccgg aaccttttct gcggaggctt
     3421 ctcttcggaa tatgatgagc ttggcacaaa aaacgtggcc tggggagaag aattcacatg
     3481 gacagcattc acgaaaccaa tccgccttct gcaaagccgc tcgccggtaa agtggctctc
     3541 gtcaccggcg gcaatcgcgg cattggcaag gccatcgcgc tccgtctggc cagcctcggc
     3601 tcggcggtcg gcatctgcgg ccgtgacaca tctaccctcg cgagcaccac gatcgagctc
     3661 ggcaccttcg acgttcgcgt ccattctcaa ccagccgacg ttacgcgggt ttacgacgtc
     3721 tccgccctcg tccagcaaac tgaagccgcg ctcggcccca tctccatcct cgtcaacaat
     3781 gccggcatgg gcctgttcgg cccggtccac gaaaaatccg agacagagtg ggacactctc
     3841 ttagacacca atctcaaaag cgtgttcctg gtttctcgcg ccgtgattcc cggcatgatc
     3901 cagcgcagat ccggcgacat catcaacatc tcctccctcg ccgggaaaaa tacctttgcc
     3961 ggtgggggca tctattgcgc ctccaagtgg gccctgcagg gcctcagcgg ttgcatggcc
     4021 gaagatttgc gcacctacgg catccgcgtc agcgccgtct gtccaggcag cgtcttcacc
     4081 gaattttccg gccgcggcgc caaggaccct tccaaagtcc tcgccgcaga cgacgtcgcc
     4141 cacgccgtcg ccatgatcgc cacgcaaggc ccgcaaagtt tcctcagcga agttcacctc
     4201 cgcccggttt ctaaaccctg atgtctctta aacccggatt tctttcaaaa gttgatgcga
     4261 tccagacatt cgaaccggcc gcgccggtgt tatcatcccc aaggattttt tcggtgtcag
     4321 ccgcttcgaa tttcggcggc aaatcgatgg ctgcatacaa aggatgaccg ggcccatgcg
     4381 tttgcaattt ctttttcctt ttgccctgct cgctatcgcg gcgccttcac gcgctgccga
     4441 ctctcccgcg cccctccgcg tagccattgc cggcttggtt cacggccatg ccgaaggctt
     4501 cttttcccat tcgctctcac gtaacgacat ccagattgtc ggtatcgccg aacccgatca
     4561 tgctctcttc gatcgctacg ccgccaaata caaactcgac gcatccttgt atcacgccga
     4621 cctcgacgaa cttctgcgca gcacccaccc gcaagccgtc ctcggctaca cctcgaccct
     4681 cgaccatcgc aaaatcatcg aaacctgtgc gcgttattcc gttcccgtaa tggtagaaaa
     4741 gccactcgcc acaacctacg aagacgccaa agccatcgcc gccgccgctc gtggcgccaa
     4801 gattcacgtc ctcgtgaact acgaaacgtc ctggtatgcg agcaaccgcg ccgcttacga
     4861 actcctccac tccggcgcca tcggcgacct ccgcaaagtc gtcgtccatg acggccacga
     4921 aggtccaaaa gaaattaacg tcgggccgga attcctaggc tggctcacgg atcccaagct
     4981 caatggcggg ggcgcgcttt tcgatttcgg ctgttacggc gccgacctga tgacctatct
     5041 catggacggc gcgcgccctc tcaccgtcac cgccgtcacc cagcgcatga agcccgatat
     5101 ctatccgcgc gtggacgatg aggcgaccat cgtcctgacc tatccgaggg cccaggcgat
     5161 tcttcaggct tcctggaact ggccctacag ccgtaaggac atggaagtct acgggcgcac
     5221 cggctccgtc atcaccatcg ccaacaatga catcaacgtc cggctcccgc acgagcgcca
     5281 ag
//
LOCUS       DASTPM010000625         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_685554, whole genome shotgun sequence.
ACCESSION   DASTPM010000625 DASTPM010000000
VERSION     DASTPM010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685554"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VFR42_11005"
     CDS             complement(<1..1608)
                     /locus_tag="VFR42_11005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0369733.1"
                     /translation="MKKSRVILLVVLLVTATVGFFFLPLRQWFTQLEGHVQSLGTLGP
                     AVVVAAYVLSTVLLIPASIITLGAGTIFGLKTGFIVVVIGANLGALCSFMLARTFLRE
                     QVARWARENSKFRFLDEAIGRQGFKMVLLTRLSPIFPFILLNYFLGLTAVRTGAYVLA
                     NLFGMLPATFLLVYIGAAARDAIGAPTDESADFYQQILKYVGLLATIAVVVTVTRMAR
                     RALREAEQSQEGSTVAGKPRLDPNYRPAAFDEMTIAGDSYDQQLIENCHPPGWVNPTP
                     AGKYNLIVIGAGPAGLVSAAGAAKLGAKVALIERNLMGGDCLNVGCVPSKAVTRAARA
                     VFDSRNGAEFGVHSNSQSEMNFAAAMERMRRLRAGISAHDSADRFRQLSVDVYIGNGR
                     FIGPSAIEVDGTRLEFSRAVIATGARAAELPIAGLADAGYYTNETIFTLTELPHRMVV
                     VGAGPVGCELAQSVQRFGCQVTVITDGAQILPKEDHDAAALLRQRLSREGVRIITSGI
                     IQQVSSNGSGKRVSVATDGTTHEVECDA"
     gene            1661..>2151
                     /locus_tag="VFR42_11010"
     CDS             1661..>2151
                     /locus_tag="VFR42_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369734.1"
                     /translation="MCLAKVSYAAARRRNHVISSHAGQVLPCFLFSLFSIQAFFQHNL
                     GWHPLCVNTLRKMSIRKFKIATIGAVLSSFLSLCVSAAFEGMAGQAHARKGRDYGGQA
                     AGDRDLERLRRIMMPLVRATNHPERLNEIRIKIVADTNINAGSAGGGEFVITTGLLQK
                     AND"
BASE COUNT          497 a          647 c          563 g          444 t
ORIGIN      
        1 ggcgtcgcac tccacctcgt gagtcgtacc atcggtggca accgacacgc gcttaccaga
       61 tccattggag ctcacttgtt ggatgatccc gcttgtaata attcgcacgc cttcccggct
      121 caatcgctgc cgcagcaagg ccgcagcatc gtgatcctct ttcggcagaa tctgggcgcc
      181 gtccgtaatc acggtcacct ggcaaccgaa acgttggaca gactgggcca actcgcagcc
      241 gacaggcccg gcgccgacga caaccattcg gtgcggcaac tccgtcagcg taaagatcgt
      301 ctcgttcgtg taataacccg catccgctag tcccgctatg ggaagctctg cggcccgtgc
      361 gcccgtggcg attacggcgc ggctaaattc gagccgcgtg ccgtccactt cgattgccga
      421 tggcccgata aaccgtccat tgccgatgta aacgtccaca ctcagctggc gaaaacggtc
      481 ggcggaatcg tgcgcgctga ttccggcccg cagccgccgc atcctctcca tcgcagccgc
      541 gaaattcatt tcggactgtg aattcgagtg tacgccaaac tctgcaccgt ttcgtgagtc
      601 gaagaccgct cgcgccgccc gagtgaccgc tttcgacggc acacaaccga cgttcaggca
      661 gtccccgccc ataagattgc gctcgatcag cgccaccttg gcaccgagct tcgccgcgcc
      721 cgccgcactc actaacccgg ccggtcccgc gccgatgaca atcagattgt actttcccgc
      781 aggtgtggga ttgacccagc ctggcgggtg gcagttctcg atgagttgct ggtcgtagct
      841 gtcaccggcg atcgtcattt catcgaaggc cgccggccta taattaggat ccaaacgtgg
      901 ctttcctgcg accgtgctgc cttcctgact ttgctcagct tcgcgcaatg ccctgcgcgc
      961 catgcgcgtg accgtcacca ccacggcaat agtcgccagt aaaccaacat acttcaagat
     1021 ttgctggtaa aaatccgccg attcgtctgt cggtgcacca atagcgtcgc gcgccgccgc
     1081 gccgatgtaa accaaaagga aagtggccgg cagcatgcca aacagattcg ctagaacata
     1141 agcacccgtg cgtaccgccg ttagccccaa gaagtagtta agcaaaatga acgggaatat
     1201 cggactcagc cgcgtcagga ggaccatctt gaaaccttga cggccgatgg cttcatcgag
     1261 gaaacgaaac ttcgagttct cccgcgccca gcgagcgacc tgttcgcgca agaaagtacg
     1321 cgccagcatg aaagagcaga gcgcgccgag gttggcgccg atgacgacca cgatgaaccc
     1381 agtctttaag ccgaaaatgg tcccggcccc caatgtgatg atacttgccg gtatcaagag
     1441 caccgtgctt agaacgtaag ccgccaccac aaccgcggga ccgagggttc caagcgattg
     1501 cacgtgacct tcaagttgcg tgaaccactg ccgcagcggc agaaagaaaa acccgactgt
     1561 cgctgtcact aggagaacaa caagtagaat gactctgctt tttttcatct tgtgtgctgg
     1621 ggaatctcgc aggttttgtt gactaactta accaatacaa atgtgcttag caaaagttag
     1681 ctatgccgca gcgagaagac gaaaccatgt aatttcttcc catgctggtc aagttcttcc
     1741 atgttttcta ttttctctct tttcgattca agcctttttt cagcacaatc taggctggca
     1801 cccactttgc gttaacactt taagaaaaat gtccattaga aaatttaaaa tagccacgat
     1861 aggggcggtg ctttcctcgt ttttgagcct ttgcgttagt gcggcatttg aagggatggc
     1921 ggggcaggcc cacgcccgaa aaggtcgaga ctacggtggg caagcagccg gcgaccgtga
     1981 cttggaacgg cttcgcagga tcatgatgcc actggtccga gcaacgaatc atccggagcg
     2041 tctgaatgaa atccgcatca aaatcgtcgc cgacaccaat ataaatgccg gcagcgcagg
     2101 gggaggcgag ttcgtcataa ccaccggtct cttacagaaa gccaacgatg a
//
LOCUS       DASTPM010000626         5023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1406532, whole genome shotgun sequence.
ACCESSION   DASTPM010000626 DASTPM010000000
VERSION     DASTPM010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406532"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VFR42_11015"
     CDS             <1..415
                     /locus_tag="VFR42_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0369735.1"
                     /translation="PQGYHTLIGERGTRLSGGQRQRIAIARAILKNSPILILDEATSE
                     LDAESEMNVQRALGNLMIGRTTFVIAHRLATIRRADMILVLEDGQIRERGTHAELMAR
                     GGTYARLHDLQFADDDMLAPAQNFQQNSSRASEPL"
     gene            412..1365
                     /locus_tag="VFR42_11020"
     CDS             412..1365
                     /locus_tag="VFR42_11020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU0369736.1"
                     /translation="MTQKSTAQASQTAAHKAECVSMTGYAQARGEQGGWAIRVSVKSV
                     NHRFLDVRFRLPEGFDVYELRLRQIVRERIHRGHIDVNVNVQPGAAVPVQVNRDLLQA
                     YAQSAEELRRQLGTTAGLDVVSLLRLPGVIGGLAPPLPETDEEQDQLGKALDACLREA
                     LLKLDDMRHAEGRHLAEELRTRVGRIGQQADQVRDLAKSLTPAFARRLQTRLKELLNG
                     TGIEPARLAQEAALLAERSDISEELDRLRSHLQQFAKLLDGAGELGKKLDFLLQEMHR
                     EANTMLSKTPGVETEALAITGLSLEIRAEIEKLREQVQNIE"
     gene            1362..2009
                     /gene="gmk"
                     /locus_tag="VFR42_11025"
     CDS             1362..2009
                     /gene="gmk"
                     /locus_tag="VFR42_11025"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU0369737.1"
                     /translation="MTAASELAPLVLIVSGPSGSGKSTLVQKILELPGTMPSVSCTTR
                     PRRATEASGKCYDFVTDAQFDAMVKRGEFLEYARVFGKHSYGTPKKWPEEARKKGLDL
                     VLEIDVQGAAQVKQKLPESVAIFILPPAREELERRLRSRGQDSDEEIKRRLARARDEI
                     AEFGKYYDYCVVNDDVERAGREAQAILIALRCTSARRRAVVEGLLASFGGKNANG"
     gene            2002..2232
                     /gene="rpoZ"
                     /locus_tag="VFR42_11030"
     CDS             2002..2232
                     /gene="rpoZ"
                     /locus_tag="VFR42_11030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU0369738.1"
                     /translation="MVNVLTKAPESQFAYVVLAARRARQLMAGAPPLLESPRTLKATR
                     IACEELENGLIEYDFVEPSSIDEDKDGNKRRK"
     gene            2340..3566
                     /gene="coaBC"
                     /locus_tag="VFR42_11035"
     CDS             2340..3566
                     /gene="coaBC"
                     /locus_tag="VFR42_11035"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU0369739.1"
                     /translation="MRITLGVTGGVAAYKAAELVRRLQQDGFTVQVVMTRGAREFVTP
                     LTFAALSGQKVITDLFAESGGEANLESAIEHIAVAQRTDLLLVAPATADILAKFARGI
                     ADDFLSTLYLASTAPVVVAPAMNVNMWNHAATQENFEMLRARGVKVVDPDEGYLACGM
                     TGAGRLAGQEAIVAAVREALHIVRDLAGETVLVTAGPTRENVDPVRYFTNRSSGKMGY
                     AVAEAAAHRGARVILISGPVSLPAPAGVERIEVESAEEMHRAVLEKVHGCSIAIFAAA
                     VADYRPAEPRDQKIKREKESLTLELEPTQDILASVARNKGDRFIVGFAAETERVAENA
                     RKKLSAKNADLMVANDVTAEGAGFDHETNIVTLFARDGRDLPLPRMSKSEVAQRILDE
                     VVRLRSSSRTPQRSTV"
     gene            3572..4423
                     /locus_tag="VFR42_11040"
     CDS             3572..4423
                     /locus_tag="VFR42_11040"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0369740.1"
                     /translation="MADRISAALQEKVSKRLNFYQDIGIDSFYRDRYPRQLASVPEET
                     TLPKSAQKTLPPKPAVQPRPAAPRLDAFPAASGNSLFEAMNKIEGDTLLKIHADIGDD
                     CTRCKLHRGRTKLVFGDGNPNAQLVFVGEGPGRDEDAQGLPFVGRAGKLLTQMIEAMG
                     LQRQDVYICNVVKCRPPENRTPEKDEVETCSPFLLRQLDAIAPKVIVCLGSVAAQTLL
                     ETNRGISHFRGQWLEFRGRKLMATYHPAYLLRNPSAKGEVWKDLQKVMAILGLQGKKT
                     AQAAKPS"
     gene            4430..>5023
                     /locus_tag="VFR42_11045"
     CDS             4430..>5023
                     /locus_tag="VFR42_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0369741.1"
                     /translation="MGILLGILTALTWGSSDFLARFATHRMGTLRAMLYMQLIGFLLL
                     TAALPFLGGWGHLADGSGWQPWAWGLLAGSVNAVSTLGLYRAFEIGKMAVVAPLSASY
                     PVLSVSLSLLSGERLSIPRAAGIACTMLGVLLVAGGEKPPDENDAEAVKRSGKGIGWA
                     LSSAVGFGLLFWLLGLRVIPRVGAPQTVWMIRLTCTVI"
BASE COUNT         1026 a         1512 c         1520 g          965 t
ORIGIN      
        1 gccgcagggt tatcacacgc tgatcggcga acgcggcacc cgcctcagcg gtgggcagcg
       61 tcaacgtatc gccatcgctc gcgccatcct gaaaaattca cccatcctga ttctggacga
      121 agctacgtcc gaactggatg ccgagtccga aatgaacgtg cagcgagcgc tcggcaacct
      181 gatgattggc cgcaccacat tcgtgatcgc gcaccgattg gcgaccattc gccgcgccga
      241 tatgattctg gtgctggaag acggccagat ccgcgagcgc ggcacgcacg cggaactcat
      301 ggcgcgcggg ggcacctacg cccggctgca cgacctgcag tttgcggacg acgacatgtt
      361 ggcgccggcg caaaacttcc agcaaaatag ttcccgggca agcgaaccct tatgactcag
      421 aaatccaccg cgcaagccag ccaaaccgcg gcacacaaag cggaatgcgt ttctatgacc
      481 ggctatgcgc aagcgcgcgg cgaacaaggc gggtgggcca ttcgtgtgag tgtaaaaagc
      541 gtgaaccatc gctttttgga tgtgcgcttt cgtttgccgg aaggcttcga tgtttatgag
      601 ctgcggctgc gacagattgt gcgcgagcgc attcaccgcg gccatatcga tgtcaacgtg
      661 aacgtgcagc cgggcgcggc cgtgccagtg caggtgaacc gcgatttgct gcaagcctac
      721 gcgcaatcgg cggaagagct tcgccgccag ctgggcacga ctgcgggcct cgacgtggtt
      781 tctttgttgc gcctgcccgg cgtaatcggt ggactggcac cgcctctccc ggaaaccgat
      841 gaagaacaag accagcttgg caaagcgctg gatgcctgtt tgcgcgaggc tctgctgaaa
      901 cttgacgaca tgcgccacgc cgaaggacgc catctggccg aagaattgcg gactcgcgtg
      961 ggccgcatcg gccagcaggc cgatcaggtt cgcgacctcg ctaagtcatt gacgccggcc
     1021 tttgcgcgcc ggctgcaaac tcgcctaaag gagttgctga acggcaccgg catcgagcct
     1081 gcgcgtttgg cgcaagaagc cgcgctactg gcagagcgca gcgacatcag cgaagagctc
     1141 gatcgcttgc gcagccactt gcaacaattc gccaagctgc tggatggcgc cggcgagctc
     1201 ggcaaaaagt tggattttct gctgcaggaa atgcaccgtg aagccaacac catgctctcg
     1261 aaaacgccgg gcgtggaaac ggaggcgctg gccatcaccg gtttatcact ggagattcgc
     1321 gcggaaatcg aaaaattgcg cgaacaggtg cagaacatcg aatgaccgcg gcgagcgagc
     1381 tggcaccgct ggtgctgatc gtgtccggtc cttcgggctc gggcaaatcg acgctggtgc
     1441 agaaaatttt ggaattgccc gggaccatgc cctcggtttc atgtactacc cggccgcgcc
     1501 gcgcgactga ggccagtggg aagtgctatg attttgttac cgacgcgcaa ttcgacgcca
     1561 tggtcaagcg tggtgagttt ctggagtacg cgcgcgtttt cggcaagcat tcctacggca
     1621 ctccgaaaaa gtggccggag gaagcgcgga aaaagggact cgacctggtg ctggaaattg
     1681 atgtgcaggg tgccgcacag gtaaaacaga agttacccga gtcggttgct atttttattt
     1741 tgccgccggc gcgagaagag ttggagcggc ggttgcgcag ccgcggccag gattcggacg
     1801 aagaaatcaa acgccggctg gcacgagcgc gcgatgaaat tgcggaattc ggaaagtatt
     1861 acgattattg cgtcgtgaat gacgacgtcg agcgtgccgg gcgtgaggcg caagccatct
     1921 tgatcgctct gcgctgcacc agcgctcggc ggcgcgcagt agtggaaggt cttctggcat
     1981 cgtttggagg aaaaaacgca aatggttaat gtgctgacga aagctcccga gagccagttt
     2041 gcttacgtag tccttgcggc gcgccgcgca cgccagttga tggcgggcgc tcctccgctg
     2101 ctggagagtc cgcggacgtt aaaggcaact cgcatcgctt gcgaagagct ggaaaacggt
     2161 ctgatcgaat acgatttcgt cgagccttca tcgatcgatg aagacaaaga cggcaataag
     2221 cgccgtaaat aggcgccatt gtaggggctg cgtttcagcg cgacccgacc caagccagtt
     2281 tcttggcgag ggttcttttt gttaagcgag ttcagccccg gaagcaaacg cagatgccaa
     2341 tgcggatcac tctcggcgta actggcggag tggcggctta caaagctgcg gaactcgttc
     2401 gccgcctgca acaggatgga ttcaccgtgc aggtggtgat gacgcgcggg gcgcgcgagt
     2461 ttgtcacccc gctgaccttt gccgctctca gcgggcaaaa agtaatcacc gatcttttcg
     2521 ccgaatccgg cggcgaagcc aatctggaaa gcgccatcga gcatatcgcc gtagcccagc
     2581 gcaccgactt gctattggtc gcgcccgcca ccgccgacat tctggccaaa tttgcccgcg
     2641 gcatcgctga cgatttcctt tcgacgttat atcttgccag caccgcgcct gtagtggtcg
     2701 ctccggcgat gaacgtcaac atgtggaacc acgccgccac gcaggaaaat tttgagatgt
     2761 tgcgcgcgcg cggcgtcaag gttgtcgacc ccgacgaagg ctacctggcc tgcggcatga
     2821 ccggcgccgg gcgcctggcc gggcaggaag cgattgtcgc cgcggtgcgc gaagcgctcc
     2881 acatcgtgcg cgatcttgcc ggagagacgg ttctggtcac tgccgggcca acccgcgaaa
     2941 atgtcgaccc tgttcgctat ttcaccaatc gttcttcggg gaagatgggc tatgcggtag
     3001 ccgaggccgc ggcgcatcgc ggtgctcgtg taattttgat cagcgggcct gtttcgctgc
     3061 ccgcgccagc cggcgttgaa cgcattgagg tggaaagcgc agaagagatg catcgcgccg
     3121 ttctggaaaa agttcatggc tgctcgatcg ccatttttgc cgctgccgtt gcggactatc
     3181 gtccggctga accccgggac cagaaaatca agcgcgagaa ggaatcgtta actctagagc
     3241 tggagcctac tcaagatatc ctggcctccg tggcgcgcaa caaaggcgat cgtttcatcg
     3301 ttggatttgc cgcagaaacg gagcgcgttg cagaaaatgc gcgcaagaag ctttccgcca
     3361 agaacgccga tctgatggtg gccaacgatg taaccgcgga aggcgctggc ttcgaccatg
     3421 aaaccaacat cgtcaccctg ttcgcgcgcg acggccgcga tctgccgctg ccgcgcatga
     3481 gcaagagcga agtcgcacag cgcattctcg atgaagtcgt gcgcttgcgc tcgtcctcac
     3541 gaaccccgca gcgttctacc gtctgaactc aatggccgac cgcatatccg cagcgctcca
     3601 ggaaaaagtc agcaaacgct taaacttcta tcaagacatc ggaatcgatt cgttttaccg
     3661 cgaccgctat ccgcggcaac ttgccagtgt tcccgaggaa accactttgc ccaagtccgc
     3721 gcaaaagacg ctaccaccaa aaccagcggt gcaaccacgg ccggcggcgc cgaggctgga
     3781 tgctttcccc gcggcttccg gcaactcgtt gtttgaagcc atgaacaaaa tcgaaggcga
     3841 cacgctgctg aagattcatg ccgacattgg cgacgactgc actcgctgca aactccatcg
     3901 cggccgcacc aagctcgtct ttggcgacgg aaaccctaat gcccagcttg ttttcgttgg
     3961 cgagggccca ggccgcgatg aggatgcgca aggcttgccc tttgtcggcc gcgccggcaa
     4021 gttgctgacg caaatgatcg aggctatggg cctgcagcgc caggacgttt atatctgcaa
     4081 cgtcgttaaa tgccgcccgc cagaaaatcg cacgccggaa aaggacgaag tggaaacttg
     4141 ctcgccattt ctgctgcgcc agctggacgc cattgccccg aaagtaatcg tctgcctggg
     4201 cagcgtcgcc gcgcagacgc tgctcgaaac caatcgcggc atctcgcatt tccgcggcca
     4261 gtggctcgaa tttcgcggca gaaaactcat ggccacctac catcccgcct acctgctgcg
     4321 caatcccagc gccaagggag aagtctggaa agacttgcag aaagtcatgg ccatcctcgg
     4381 cctgcaaggc aagaaaaccg cgcaagccgc gaagccttcc tgacccatca tgggaatttt
     4441 gctgggcatc ctgaccgcgc tgacgtgggg cagttcggat ttcctcgcgc gcttcgcgac
     4501 gcaccgtatg ggcacgctgc gcgccatgct gtacatgcag ttgatcggct tcctgttgct
     4561 gaccgctgcg ctgcctttcc ttggtggctg gggccatctt gcggacggtt ccggctggca
     4621 gccatgggcc tggggcctgc tcgccggtag cgtcaacgcc gtctccacct tgggtctcta
     4681 ccgcgcgttc gaaatcggca aaatggccgt ggtcgctccg ctctcagcaa gctacccggt
     4741 gcttagtgtc tcgctctcat tgctttccgg tgagcgtctc agcatcccgc gcgccgcagg
     4801 aatcgcctgc accatgctcg gcgtcttgct cgtcgccggc ggcgagaagc cccccgatga
     4861 aaatgacgcc gaagccgtga agcgcagcgg caagggtatc ggttgggcgc tgtcctccgc
     4921 agtcggcttc ggcttgcttt tctggctcct tggccttcgc gtcatcccac gggttggcgc
     4981 tccgcaaacc gtctggatga tccgcctcac gtgcaccgtc atc
//
LOCUS       DASTPM010000627         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_368467, whole genome shotgun sequence.
ACCESSION   DASTPM010000627 DASTPM010000000
VERSION     DASTPM010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368467"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFR42_11050"
     CDS             <1..996
                     /locus_tag="VFR42_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU0369742.1"
                     /translation="PRLAVMAEWAAGGKIDGNDEAARKASAARSDPIKRFLSEESLNS
                     LSSLDRVELLSELEQRYNIEINEADFSQATSAVELSRLVEKAGAEELKDSGSRASRYQ
                     YPRWAQWKPIRWLRLLVYYLLVWPATQILAHPRIVGRGNLRGVRGPVIVVSNHITRRA
                     DMGLILAALPMRFRHRLATAIGGETLLRMRTPMREWHFAKRWAYQLGYWLVTLLFNVF
                     PLPQFSGFRQSFRFAGASADRGYSVLIFPEGEVNNSEDGRMAPFRPGIGLLAENLGLP
                     VVPMRLDGVWQMKRERRRLAHLGEITVRIGAPITFVDGTPPAEFAQRLATIVKGL"
     gene            complement(1006..2094)
                     /locus_tag="VFR42_11055"
     CDS             complement(1006..2094)
                     /locus_tag="VFR42_11055"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0369743.1"
                     /translation="MIPVATGFSNPLDIQQPQDNSGRLFVVEQGGHIQIIQNDGTRAA
                     SPFLDVSTRTGFTSGGETGLLGLAFHPLYSQNRRFFVNYTRTISGQLQSIIAEFTASS
                     SDPNFTDANTENILFTVNQPFTNHKGGGLAFARDGFLYIGLGDGGSGGDPQCNAQNLN
                     VLLGKMLRIDVDGPHSPGLNYAIPATNPFVGQNARGEIWLYGLRNPFRFSFDTANGNL
                     WIGDVGQNSFEEVDMLTPQQGGANMGWNLREGTHPFSTACMQTGNTLTDPVFDYDRSQ
                     GDETVIGGHVYHGTKMPALSDTYVFGDFISGRVWSFEQTGQTFARSNVTTTSGGDLAA
                     IGEDQAGELYLARYSTGVIARIHQTGQP"
BASE COUNT          509 a          578 c          884 g          540 t
ORIGIN      
        1 ccgcgattgg ctgtgatggc cgagtgggcg gcaggtggaa agattgatgg aaacgacgaa
       61 gccgcgcgaa aggccagcgc cgcgcgatca gatccaatta agagatttct ctccgaagag
      121 tcactgaact cgctgtcttc cctggatcgg gtggaattgc tgtccgagtt ggagcagcgc
      181 tacaacatag aaatcaacga ggcagatttt tcgcaagcca catcggcggt cgaattgagc
      241 aggcttgttg aaaaggcggg agcagaagaa ttaaaagaca gtggttcgcg ggccagccgc
      301 tatcagtatc cgcgatgggc ccaatggaaa ccgattcgct ggttgcggct gctcgtgtac
      361 tacctcctgg tatggccggc gacgcaaatt ctggcacatc cgcggattgt ggggcgcggg
      421 aatttacggg gcgtaagagg tccggtgatt gtagtttcga accacattac gcgtcgcgcg
      481 gacatgggat tgattctggc ggcgcttccg atgcgttttc ggcaccgact ggcgacggcg
      541 ataggaggag aaacgttgct gcgcatgaga acgcccatgc gtgaatggca tttcgcgaag
      601 cgctgggcat accagctggg ctactggctg gtgacgctgc tgttcaatgt gtttccgctg
      661 ccgcaatttt cggggttccg gcaaagtttt cggtttgcgg gagcgtcggc ggatcgcggg
      721 tattcggtgc tgatttttcc ggagggagaa gtgaacaatt cggaggacgg gaggatggcg
      781 ccgtttcgac cgggcattgg cttattggcg gagaatttgg gattgccggt ggtgccgatg
      841 cgcctcgatg gggtgtggca gatgaagcgg gagcggcgac gcctggcgca tcttggagaa
      901 atcacggtgc gcatcggagc gccgattacg tttgtggacg gcacgccgcc ggcggagttc
      961 gcgcagcgcc tcgcaaccat cgttaaggga ttgtagtatc ggtttttacg gctggccggt
     1021 ttggtggatt ctggcgatta cgccggtgga gtagcgggcg aggtatagtt cgccggcctg
     1081 gtcttcgccg atcgcggcga ggtcgccgcc agaggtggtg gtgacgttgg atcgggcgaa
     1141 ggtctggccg gtctgctcga aagaccagac tcggccgctg atgaagtctc caaaaacgta
     1201 agtgtcagaa agcgcgggca tttttgtgcc gtgatagacg tggccgccga tgacggtttc
     1261 gtctccttga gagcggtcgt agtcaaaaac cggatcggtc agagtgtttc cggtttgcat
     1321 gcacgccgtg gagaagggat gggtgccttc gcgcagattc cagcccatgt tggccccgcc
     1381 ttgctgcggg gtgagcatgt ccacttcttc gaaggagttc tgaccaacgt cgccgatcca
     1441 gaggttgccg tttgcggtgt caaaggagaa gcggaacgga ttgcggaggc catacagcca
     1501 gatttcgccg cgcgcatttt gtcccacgaa aggattggtc gcggggatgg cgtagttgag
     1561 gccgggcgaa tgaggcccat caacgtcaat gcgcagcatt tttccgagca ggacattgag
     1621 gttctgcgcg ttgcactgtg gatcgccgcc gctgccgccg tcgccgaggc cgatgtagag
     1681 aaagccatcc cgagcgaagg cgaggccgcc gcctttgtgg ttggtgaaag gctgattcac
     1741 ggtgaagaga atgttttcgg tgttggcgtc ggtgaagttg ggatcgctgg aggaagcggt
     1801 gaattcggcg atgatggatt gcaattggcc ggaaatggtg cgcgtgtagt tcacaaagaa
     1861 gcggcgattt tgggaataca ggggatgaaa agccaggccg aggaggccgg tttcgccgcc
     1921 gctggtgaag ccggtcctgg tgctgacatc gaggaagggt gaggcggcgc gtgtgccgtc
     1981 gttttgaatg atctggatgt gtccaccttg ttcgacgacg aagaggcgtc cgctattgtc
     2041 ttggggctgc tgaatatcga gcggattgga aaagccggtg gcgacgggaa tcaaatcaaa
     2101 agtggccgga gcaggcggag gcacgggcgg aggagttggg gcggagctgt agctgcctcc
     2161 acagcccgcc gaaaagatgg ccaggagaaa aaaggtctca cgagaaagtc gacgcttaag
     2221 gaatggagtg gcgcaggcgg tcacgaattt cccccgaatg gtgtctatgt tgccctgtaa
     2281 agccgaacag gtctagccgg gattgcgtgt aaagaattgt aaggggcttg atatttctca
     2341 aggatgtctg gggcgtgtaa cgccgaagca atttcgctgg ccgatgttta cattcgagcc
     2401 gaaagttagg aaatgctgat cggtggcgaa gtcgcgaggc gctttcgcat aaagatagtg
     2461 gcttgcggat tgtcgttgta gcgctcgcac ggctgaaaac cgttgcgctg g
//
LOCUS       DASTPM010000628         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1176097, whole genome shotgun sequence.
ACCESSION   DASTPM010000628 DASTPM010000000
VERSION     DASTPM010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176097"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFR42_11060"
     CDS             <1..979
                     /locus_tag="VFR42_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0369744.1"
                     /translation="EELASGKGVIDRELDHASGRKLVRMQPAAGSDWIVTQIKDKTPP
                     GDAATQLTVQELLQEREITYRNLLAAYLKLQEVNRQKTVFLASAAHELKTPLAVIKGY
                     YDLLLAGSLGRLTEKQKDILEESKESCERLVRLVSMFLNYSALESGKLVLQLRENDLR
                     DCLDEMAKRWAEAFQRKNVRLEVQLDPSIPTFRFDYQKVQQAAANLLDNALKHTPSGG
                     NVTLVAKPHFWERRVADVAPNQERRRFRLPRPNSVEVSVTDSGGGIPAEHHQEIFEDF
                     VRVDRNTSGMGLGLAIAKRLVQAHRGKIWVESETKRGSTFRFLLPIEQS"
     gene            1017..1436
                     /locus_tag="VFR42_11065"
     CDS             1017..1436
                     /locus_tag="VFR42_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369745.1"
                     /translation="MAVQEKILVVDDEPSIRKYLQTLLEVDGFEVEAVSSGREAIKRI
                     NDGLHPDFVILDVLMPEMNGIDTLKELMQLDRSLNVIMLSCSNEVGTVVEAIRIGAHD
                     YLTKPFEKSELDAAMLKSRQKRNWQQKTRPCATTAIR"
     gene            1412..2470
                     /locus_tag="VFR42_11070"
     CDS             1412..2470
                     /locus_tag="VFR42_11070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0369746.1"
                     /translation="MRDYCDQVTEDLSFLAASPQMVRIRQQILQIAPVDVPVFVCGES
                     GVGKEVVARMIHLRSKRRNQAFIKVNCAALPGELLESELFGFEQGAFTGAVRSKPGKF
                     ELANKGTIFLDEIAEMSTHLQAKLLHVLQDHQYSRLGGRHMIETDVRVLAATNVDVHE
                     AMKTGKFREDLYYRLNVLSILVPPLRERTAEIPLLFRHFLEKYSEKFGKPPAEPSKHL
                     LDAALNYPWPGNLRELENFVKRYVILEDDEGSLRELVEMSATRQRTSPREEPVIPKEQ
                     GLKALVRGLKDEAEMEAIADALEKTRWCRKDAAKMLGISYKALLYKMRQFNLDTPRNR
                     RSPVAAKETVPVPAASTE"
BASE COUNT          614 a          654 c          771 g          485 t
ORIGIN      
        1 ggaggagctg gcctctggaa aaggcgtgat tgatcgcgaa ctggatcatg cgagcggaag
       61 aaaactggtg cgcatgcagc cggcggcggg atcggattgg attgtcacgc aaatcaagga
      121 caaaactcct ccaggtgatg ccgcaacaca attgacggtg caggaacttc tgcaggagcg
      181 cgaaatcact taccgcaacc tgctggcggc gtatttgaag ttgcaggaag tgaaccggca
      241 gaagacggtg tttctggcgt ctgccgcgca cgaactgaag acgccgctcg cggtcatcaa
      301 gggatattac gacctgctgc tggccggttc acttggccgc ctgacggaaa agcagaaaga
      361 cattctggaa gaatcgaaag aaagctgcga acgactggtg cggctggttt cgatgttcct
      421 gaactattcc gcgctggaaa gcggcaaact ggttttgcaa ttgcgcgaaa atgacttgcg
      481 cgattgtctg gacgaaatgg cgaagcgttg ggccgaagcg ttccagagga aaaacgtccg
      541 tctggaagtg caactcgatc catccattcc gacgtttcgt ttcgattatc agaaggtgca
      601 gcaagccgca gcgaacctgc tggacaacgc gctgaagcat acgccgtcgg gtggaaatgt
      661 gacgctagtg gccaagccgc atttctggga acgccgggta gctgacgtag ctccgaatca
      721 ggagcgacgg aggttccgcc tgccgcgccc gaacagcgtc gaagtcagcg tgacggattc
      781 aggtggtgga attcccgccg agcatcacca ggagattttt gaagactttg tgcgagtaga
      841 tcgtaacacc tcaggcatgg gcctcggatt agcgatcgcc aaacgcctgg tgcaagcgca
      901 ccgcggaaag atttgggtcg agagcgaaac caagcgaggc agcacgtttc gtttcctgct
      961 gccgatcgaa cagagctgaa gccacctcga caaaattttg agaaaggaca atgaagatgg
     1021 cggttcaaga aaagattctg gtggtggacg acgagccgag catacgcaag tatttgcaga
     1081 cgttgctcga agtggatggt tttgaagtcg aggcggtaag cagcggccgg gaggccatca
     1141 agcggatcaa cgacggcttg catccggatt tcgtgattct ggatgtgctt atgccggaga
     1201 tgaacggtat tgatacgctg aaggaactaa tgcagctgga ccgttccctc aacgtcatca
     1261 tgctgtcgtg ctcgaacgaa gtgggcacag tggtggaggc aatccgcatc ggcgcgcatg
     1321 actatctgac caagccgttt gagaaatcgg aactggacgc cgccatgctg aaatcgcgcc
     1381 aaaaaaggaa ttggcaacag aaaaccaggc cttgcgcgac tactgcgatc aggtgaccga
     1441 ggatctcagc tttctcgccg ccagtccgca gatggtgcgc atccgccagc aaattctgca
     1501 gattgcgcct gtcgacgtgc cggtatttgt ttgcggtgag agcggtgtcg gcaaagaagt
     1561 cgtggcccgc atgatccatc tccgttcgaa gcgccggaat caggccttca ttaaggtgaa
     1621 ctgcgcggcc cttcccggcg aactgctgga gtcggaactg tttggattcg aacagggggc
     1681 ctttaccggg gcggtgcgct ccaagccggg aaagtttgag ttggccaata agggcactat
     1741 cttcctggac gaaatcgcgg agatgagcac gcatctgcag gcgaagctgc tgcacgtttt
     1801 gcaggaccac cagtattcgc ggctgggcgg ccgccacatg atcgaaaccg atgtgcgagt
     1861 gctggcggcg accaatgtgg atgttcacga agcaatgaaa actggcaagt tccgcgagga
     1921 cctctactac cggttgaacg ttctatcgat tctcgtcccg ccgctgcgcg aacgcacggc
     1981 ggaaatcccg ctgctgttcc ggcacttcct ggagaagtac agcgaaaaat ttggcaagcc
     2041 gcccgccgag ccgtccaagc acttgctgga tgccgcgttg aactatccgt ggcctggcaa
     2101 tctgcgcgaa ctggaaaatt tcgtcaagcg ctacgtcatt ctagaagacg acgaaggcag
     2161 cttgcgcgaa ttggtggaga tgtcagcgac acggcagcgc acttcgccgc gcgaagaacc
     2221 ggtcattccc aaagagcagg gactcaaagc gctggtgcgc ggcttgaaag atgaagcaga
     2281 aatggaagcc attgccgacg cgctggagaa aacaaggtgg tgccgcaagg atgccgccaa
     2341 gatgctgggc atcagctaca aagcgctcct ttacaagatg cggcaattca atctggatac
     2401 gccgcgcaac cggcgttcgc cagtggcagc caaggaaacc gtgcccgtgc ccgctgcctc
     2461 cacggaatag cgctcaacgg cgtagagata catttcaaac aaccaccagc aaattttgct
     2521 aggt
//
LOCUS       DASTPM010000629         5369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_137650, whole genome shotgun sequence.
ACCESSION   DASTPM010000629 DASTPM010000000
VERSION     DASTPM010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137650"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..908)
                     /locus_tag="VFR42_11075"
     CDS             complement(147..908)
                     /locus_tag="VFR42_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369747.1"
                     /translation="MSKATKKLRTLLVDDEELARKVLRELLSAHDEIEIVAECKNGLE
                     AVKSVAEHKPDLLFLDVQMPKLTGFDVLELIGTELSVIFVTAYDEYAMKAFEVHAVDY
                     LLKPVGKDRLAAALQRVSVRSKEKLPAPAELTAASRPAGQFLERLVVKDGTRVTLIPV
                     AKLDYAEAQDDYVALASEGKKHLKQQTIASLEAALDPKQFVRVHRSYVVNLERVIRIE
                     PYGKDSRVAVLSMGVRLPVSRAGYSRLQALLDERH"
     gene            complement(905..1978)
                     /locus_tag="VFR42_11080"
     CDS             complement(905..1978)
                     /locus_tag="VFR42_11080"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU0369748.1"
                     /translation="MADSVKPRRSFPGRINQFEWVGVAVVLIPALATLGNLSWINSTA
                     LGVPLALFSLFIPLAAGFVCKFTPLDRSVLWRTVLTHVMAAQVLSFLWTLIAGPFARA
                     LSYIPQFRGLDKQFSADLWIVYVTGSLVYLMSIAFQYVVIAQEAARAVELRAVETSML
                     ARDAELRALRAQINPHFLFNSLNSISALTSIDPARAREMCLLLADFLRLTLGMGEKHV
                     IRFSEELDLLEKFMAIEKVRFGERLHMQENIQEEAKRCQIPALLLQPLLENAVSHGIA
                     SMADGGQIRLQAQVQDGRLGIVVENDRDDEAPPRRKSGVGLKNVRSRLEARYGKDATF
                     RIEPGEDRFRVSMTLPAEYGENT"
     gene            complement(1986..2813)
                     /locus_tag="VFR42_11085"
     CDS             complement(1986..2813)
                     /locus_tag="VFR42_11085"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEU0369749.1"
                     /translation="MDNDEKEWRKPPAWKVDDVREEIRDKVRRQVNDHIKVGIRSRGC
                     RNRGWGGLIPGAIILAVGIIFLLDSLGYVRARHFLQFWPMILIFVGIAKIARRDSRIW
                     GALVLLFGIFLQLSELGIGHFSWGQFWPLLLIAGGAMAMWSAIQARRLMPTASSDTTD
                     PRDTLDESAIFGGIEKRLNSREFRGGRLQAIFGGIELDLRDADMLGNEAVIHANAIFG
                     GIEMRVPETWFVATRGQGVFGGFSDSTRFLPPADPEKLKKTLIVLGTSLFGGVEIRN"
     gene            complement(2873..3178)
                     /locus_tag="VFR42_11090"
     CDS             complement(2873..3178)
                     /locus_tag="VFR42_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369750.1"
                     /translation="MVAVLEGSAARKQKAGQVLLAIGGVALLMQLWALWLEASAAWSR
                     SAAESLGWLGALGLAMLQMVDFIAWNPNGILLSVARLLLLCCPVALMMAGAAFSRRS"
     gene            3455..3973
                     /locus_tag="VFR42_11095"
     CDS             3455..3973
                     /locus_tag="VFR42_11095"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0369751.1"
                     /translation="MSPDRSRLASFRSHSWRWVLYVVLALFGGSLTFVGARAQQKATE
                     EATFRKLIDDYCAAWSSGNADNAAKYYAQEEGFVFYDVAPFSYQGWKQYRVGVQKNLF
                     DNLNAAKLTAGKDLKVTKRGMIAWTTVPMHLSTTGKDGKTSEYEIRYTGIWEKRGARW
                     LLVHEHLSAPLG"
     gene            4028..4264
                     /locus_tag="VFR42_11100"
     CDS             4028..4264
                     /locus_tag="VFR42_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369752.1"
                     /translation="MWNALTYYWIAARGFRLHPWDSPYLRWRLETFLGAEAESADAAQ
                     FLKILWKYRHQMQNFVDWAAERRRVQARSGTPSN"
     gene            complement(4280..4783)
                     /locus_tag="VFR42_11105"
     CDS             complement(4280..4783)
                     /locus_tag="VFR42_11105"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0369753.1"
                     /translation="MKLTELFLGQLQSEEGSTRKVLQRVPEGRNDWKPHEKSMPLGYL
                     SALVAGMPGWIDFMVNRDELDIASPGGAQFKPQVPDSNRELLSAFEQNLSKARAALQA
                     TSDEHLHKPWKFIVAGHVADERPRYMMIRDAVFSHMAHHRGQLTVYLRLNEAKVPSIY
                     GPSADER"
     gene            4890..>5369
                     /locus_tag="VFR42_11110"
     CDS             4890..>5369
                     /locus_tag="VFR42_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0369754.1"
                     /translation="MSELNVLQVSPQLHEAQWWEVDPNGKVHCYLCPRHCHIGRGQAG
                     FCFIRTNQEGKLYSLGYGAPAALQIDPIEKKPLNHFLPGTSVFSMGTAGCNMGCFFCQ
                     NWDISKSRQDQVNARDIAPEEVPLLALRHGCDSLAFTYNEPTIWGEYIIDICKAAKPY
                     "
BASE COUNT         1134 a         1567 c         1530 g         1138 t
ORIGIN      
        1 aagcttgcga atcaggaagt gaaggcacgc ggatttatca agcgtcggaa gggtgtagaa
       61 cgaaaagatt gggtggtgct tgaacttacg agcgtcggcc tgaagtcacc gacgggcacc
      121 acctgagaac tctccgaaaa attcatctaa tgtcgttcgt ccaatagtgc ttgcaggcgg
      181 ctatagcccg cgcggctgac cggaagccgc acgcccatgg acagcacggc aacgcggctg
      241 tccttgccgt aaggctcgat gcggatcaca cgttcaagat tcaccacgta agagcggtgg
      301 acgcgcacaa attgtttggg atcgagcgct gcttcgaggc tggcgatggt ttgctgcttg
      361 agatgctttt tgccttcgct agccagtgcg acgtagtcgt cctgcgcttc ggcgtaatcg
      421 agtttcgcga ccgggatcag ggtcacgcgg gttccgtctt ttacgaccag ccgctccaga
      481 aactgtccag ccggacgcga ggccgccgtc aattccgcgg gagcgggaag tttttccttt
      541 gagcgcacgc ttaccctttg cagcgcggcc gcgagccggt ctttcccgac cggtttcaac
      601 aaatagtcga ccgcatgcac ttcgaacgcc ttcatggcgt actcgtcgta ggccgtcaca
      661 aaaatcacgg aaagttcggt gccgatcagt tccagaacgt cgaaaccggt cagcttgggc
      721 atttgcacgt cgagaaacaa caaatccggc ttgtgctcgg cgactgactt gacggcttct
      781 aaaccattct tacattcggc gacaatttca atttcgtcgt gcgcactcaa cagttcacgc
      841 agtactttac gcgctagttc ttcgtcgtcc acaagcagcg tgcgcaattt cttggtcgcc
      901 ttgctcacgt gttctctccg tattcagcgg gtagtgtcat actcacacga aagcggtctt
      961 cgccgggttc aatgcggaaa gtagcgtctt tgccgtagcg ggcttccagc cggctgcgca
     1021 cattcttaag tcctacgccg ctttttcgcc gcggcggagc ttcgtcgtcc ctgtcgtttt
     1081 ccaccacgat acccagtcgg ccgtcttgca cctgagcttg cagccgaatc tgcccgccgt
     1141 ccgccatcga agcaattcca tggctgacgg cattctccag cagcggctgc agcagcagcg
     1201 ccggaatctg gcagcgtttc gcttcttcct gaatgttttc ttgcatatgc aggcgctcgc
     1261 cgaatctcac tttttcaatg gccatgaatt tttccaggag atcgagttct tcggagaagc
     1321 gaatgacgtg cttttcgccc atgcccagcg ttaatcgcag aaagtccgcc agaagcaggc
     1381 acatttcccg cgcgcgagcg ggatcgatgc tggtcagagc gctgatcgag ttcaaactgt
     1441 tgaacaggaa atgcggatta atctgtgcgc gcagggcccg cagctccgcg tcgcgcgcca
     1501 gcatactagt ttccaccgcg cgcagttcca ccgccctcgc tgcctcctgc gcaatcacca
     1561 catactgaaa agcgatggac atcaagtaga ccagcgatcc ggtgacatac acgatccaaa
     1621 ggtcggcaga gaactgtttg tccagtccgc gaaactgtgg aatgtagctc aatgctctgg
     1681 cgaaaggacc cgcgatcagc gtccacaaaa agctcaggac ttgcgcggcc atcacatgcg
     1741 tcaacacggt acgccacaag acggaccgat cgagcggcgt aaacttgcag acgaatcctg
     1801 ccgctagcgg aataaaaagc gagaacagcg ccaatggcac tccgagcgcc gtgctgttga
     1861 tccaggacag gttgccaagc gtggccagcg ccggaatcag aaccaccgca acgcccaccc
     1921 actcgaattg gtttatgcgg ccggggaagg agcgccgcgg ttttacggag tccgccatgg
     1981 cagtctcagt tgcggatctc cacgcctcca aacagcgaag tgccgaggac gatcaaagtc
     2041 tttttgagtt tctcaggatc ggctggaggc aagaagcgag tggaatcgga gaatccgccg
     2101 aaaacgccct gtccgcgcgt ggccacgaac caggtttccg gcacgcgcat ttcaattccg
     2161 ccgaagatgg cgttcgcatg aatcaccgct tcgtttccca acatgtcggc gtcgcgcaaa
     2221 tcgagttcga tgccgccgaa aatagcttgc aggcggccac cgcgaaattc gcggctattc
     2281 aggcgctttt cgatgccgcc aaagatggcg ctctcgtcca gagtgtcgcg cgggtcggta
     2341 gtgtcgctcg aggcggttgg catcagcctg cgtgcttgga tggcgctcca catggccatg
     2401 gcgccgcctg cgatcagcag caagggccag aactggcccc agctaaagtg gccgattccg
     2461 agttcgctca gttgcaggaa aatgccaaac aggagcacca gcgcgcccca gatgcgcgaa
     2521 tcgcggcgag caatcttggc gatgcccaca aaaatcaaaa tcatcggcca gaattgcaaa
     2581 aagtgccggg cgcgaacgta gcccaggctg tccagcagaa aaatgatgcc gaccgcgagg
     2641 atgattgcgc cagggattaa accgccccag ccacggttac ggcaccctct ggaacggatt
     2701 cccactttga tgtgatcgtt tacttggcgc ctcactttgt cgcggatctc ctcgcggacg
     2761 tcgtccactt tccaggcggg aggcttgcgc cactcttttt cgtcgttatc catttgcctg
     2821 tccttacgtc accgaatatc gaactattcg tagaaagctc gcaactgctt ctctagcttc
     2881 tgcgcgagaa tgccgctccc gccatcatga gcgccacggg gcagcataat agcagcaggc
     2941 gagccacact cagcaaaatc ccattcgggt tccaggcaat gaagtccacc atctgaagca
     3001 tggctaggcc cagggcaccc agccagccca gcgattccgc cgcgctccgg ctccatgccg
     3061 cgctcgcctc cagccacaag gcccacaatt gcattaacaa cgcaacgcct ccgatggcga
     3121 gcagcacttg tcccgctttt tgcttcctcg ccgcgctgcc ttcgagtacc gcaaccatgg
     3181 ccgcctccac tcccgctatt ccttgcgggc aggcccagaa taggggcagc ccggagggca
     3241 tgcagcagca tttcggcgga tgggggtcgc ctaccggcga atgacggcgg gctctcggtc
     3301 tgcggcggcc cggcgaaaac ggagaaatgc gcggattaga gcagcagtgg aaaacgcggc
     3361 gataccgctt gcaaaatcaa aagcttatcg atagctttag ggacactttt tcgagcctaa
     3421 cgaagggcat tttaatctcg aggagtatct gaacatgagt ccggacaggt cccggctggc
     3481 ttcttttcga tcgcatagtt ggcggtgggt tctgtatgtg gttctggcgc tgtttggcgg
     3541 atcgctgacg tttgtcggcg cgcgcgccca gcagaaagcc accgaggaag cgacgtttcg
     3601 caagttgatt gacgattact gcgcggcgtg gagctccggc aatgccgaca acgccgcgaa
     3661 atactacgcg caagaagaag gcttcgtttt ctacgacgtc gcgccgttct cttatcaggg
     3721 ctggaaacag tatcgcgtcg gcgtacagaa aaatcttttc gacaatctca acgccgccaa
     3781 gttgacggcg ggcaaagatc tgaaagtgac caagcgcggc atgatcgctt ggacaacggt
     3841 cccgatgcac ctcagcacga cggggaaaga cgggaaaacg tcagaatacg aaatccgcta
     3901 tacgggtatc tgggaaaagc gcggcgcgcg ctggctattg gtgcacgaac atctttccgc
     3961 tccgctgggc taaactgact cgggggccgc ggaacgcgaa cttaaggcgc cgtcgtattt
     4021 acctttcatg tggaatgctt tgacctatta ctggatcgcc gcgcgcggat tccggctgca
     4081 tccctgggat agtccctatc ttcgttggcg cttggaaacc tttctgggag cagaggcgga
     4141 gagcgccgat gccgcgcaat tcctgaagat tctctggaag taccgccacc agatgcaaaa
     4201 cttcgtggat tgggcggcgg agcgccggcg cgttcaagcg cgctcgggaa caccctcaaa
     4261 ttagtccacc cgctatcgct cagcgttcgt cggcggatgg gccgtagatg gatggaactt
     4321 tcgcttcatt cagccgcaaa tacactgtca gctgacctcg gtggtgcgcc atgtggctga
     4381 acacggcgtc gcgaatcatc atgtagcgcg ggcgctcgtc cgccacgtgg ccggcgacga
     4441 taaatttcca aggcttgtgc aaatgttcgt cgctggtcgc ctgcagagcg gcgcgggctt
     4501 tggataagtt ctgttcaaag gccgagagta actcgcggtt gctatccggc acctgaggct
     4561 tgaactgggc gcctcccggc gaggcgatgt ccagttcgtc gcggttcacc atgaagtcga
     4621 tccatcccgg catgccggct accagcgcgg aaaggtaacc cagaggcatg gacttttcgt
     4681 gaggcttcca gtcgttgcgt ccttcgggga ctcgctgcaa gactttacgt gtgctgccct
     4741 cttccgattg cagctggccc aaaaacaatt ccgtgagttt catggaacgc tcctgtgaat
     4801 tagtcacagg ctagcatccc gccctttggg tgtcagccgt ctttcggccc gtcgctgggg
     4861 ttatactcat gcttaaggag gatatgtata tgtcggaatt gaacgtcctc caggtttcgc
     4921 cacaattgca cgaagcgcag tggtgggaag tggacccaaa cggcaaggtc cactgctatc
     4981 tttgtccgcg gcactgccat atcggacgcg ggcaggcggg tttctgtttt attcgcacca
     5041 accaggaggg gaagctttat agcttgggat acggcgctcc cgctgcgttg cagattgatc
     5101 ccatcgagaa aaagcctctg aatcattttc tgcccggtac gtcggttttc agcatgggaa
     5161 ccgctggctg caacatgggc tgcttttttt gccaaaattg ggatatttcc aagtcccggc
     5221 aagatcaggt gaacgcgcgc gacattgcgc cggaggaagt tccgttgctg gcgctgcgcc
     5281 acggctgcga ctctctggcc ttcacttaca acgagccgac gatctggggc gagtacatca
     5341 tcgatatttg caaggcggca aaaccgtac
//
LOCUS       DASTPM010000630         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_973886, whole genome shotgun sequence.
ACCESSION   DASTPM010000630 DASTPM010000000
VERSION     DASTPM010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973886"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /gene="lpxK"
                     /locus_tag="VFR42_11115"
     CDS             complement(<1..451)
                     /gene="lpxK"
                     /locus_tag="VFR42_11115"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU0369755.1"
                     /translation="MASSLESTLTRAWLRRGPLALALWPLSIVFRLLATLRATLFRAG
                     ILKSERLPVPVIVVGNIFIGGTGKTPLTIWLVEQLRAAGMRPGVISRGHGGEGESGPR
                     EVLPGASAREVGDEPLLIAARAGCPVVVGRRRIEAGRHLLQLHPEVDV"
     gene            complement(632..1087)
                     /locus_tag="VFR42_11120"
     CDS             complement(632..1087)
                     /locus_tag="VFR42_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU0369756.1"
                     /translation="MMNFRRGRKREDPEINLIPFIDVLLVVLIFLMVTTTYSKFTELQ
                     ITLPTADAEKAIDKPFEINVTVDAKGNYTVNNVPVSFHGVAGLSEDLKRAAATSPDGQ
                     QLPTPARDPVIIVNADQFAMHQMVINVLEAARMAGYEKLTFAAQSGGQK"
     gene            complement(1084..1689)
                     /locus_tag="VFR42_11125"
     CDS             complement(1084..1689)
                     /locus_tag="VFR42_11125"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU0369757.1"
                     /translation="MLAILQAAGWPIWLLLIASIIALALIVERVIYLRREKILPRQLL
                     DEVIQVYRNGKVTPDIIDKLQTNSPLGAVLAAALRNVDAPRDVMKESIEEAGRGVAHV
                     LERFLTTLGTIASLAPLMGLFGTVVGMIEIFGAQNATGTNPAQLAHGISVALYNTGFG
                     LAIAMPALVFYRHFRALTDSFIMDMELQAVKFVDVVHGARK"
BASE COUNT          562 a          727 c          779 g          503 t
ORIGIN      
        1 ggacgtcgac ctcggggtgc agttgcagca ggtgacgccc ggcctcgatc cggcgccgcc
       61 cgaccaccac cgggcagccg gcccgcgccg cgatcagcag cggctcgtcg ccgacctcgc
      121 gcgcgctggc gccgggcagg acttcccgcg ggcccgactc gccttccccg ccatggccgc
      181 gcgaaatcac cccgggccgc atgccggccg cgcgcaattg ctcgaccagc cagatggtca
      241 gcggcgtttt accggtaccg ccgatgaaga tattgccgac cacgatgacc ggcaccggca
      301 ggcgttcgga tttcaggatg ccggcgcgga acagggttgc gcgcagggtt gcgagcaggc
      361 ggaagactat cgacagcggc cacagcgcca gggccagggg gccgcggcgg agccaggcgc
      421 gggtgagtgt ggattcgagg gaggatgcca tgtaaggatg tgagcaaaaa acgttagctc
      481 aaaaacgtcg tccccgcgaa ggcggggatc catactgaac aacagaaatc ggtatgcccg
      541 aagcttgtca atgcttcaga aataccgatt tcgaatactc agcatgggtt cctgctttcg
      601 cgggaacgac ggattaccgc gggacgacga attatttctg cccgccgctc tgcgccgcaa
      661 acgtcaactt ttcatacccc gccatgcgcg ccgcttcgag cacattgatc accatctggt
      721 gcatggcgaa ctggtcggcg ttgacgataa tcacagggtc ccgggccgga gtgggaagct
      781 gttggccgtc cggggaggta gcggccgcgc gcttcaggtc ttcggacaag ccggccacgc
      841 cgtggaagga caccggcaca ttgttcaccg tgtaattgcc cttggcgtcg acggtgacgt
      901 tgatctcgaa cggcttgtcg atcgcctttt cggcgtcggc ggtcggcagc gtgatttgca
      961 gttcggtgaa cttgctgtag gtggtcgtga ccatcaggaa gatcagcacc accagcagca
     1021 cgtcgatgaa cgggatcagg ttgatttccg gatcctcgcg cttgcggccg cggcggaaat
     1081 tcatcatttg cgggcaccgt ggacgacgtc gacgaatttc acggcctgca actccatatc
     1141 catgatgaag ctgtcggtca gggcgcggaa gtggcggtag aacaccagtg ccggcatcgc
     1201 gatggccagg ccgaagccgg tgttatacag ggccaccgaa atgccgtgcg cgagctgggc
     1261 cggattggtg ccggtcgcgt tctgggcgcc gaagatctcg atcatgccga ccaccgtgcc
     1321 aaacaagccc atcagcggcg ccagcgaggc gatcgtgccc agcgtggtca ggaaacgctc
     1381 gagcacgtgg gccacgcccc tgcccgcctc ttcgatcgac tctttcatga catcgcgcgg
     1441 cgcgtcgacg ttgcgcagcg ccgcagccag cacggcgccc agcggcgaat tggtctgcag
     1501 cttgtcgatg atgtcgggcg tgaccttgcc gttgcgataa acctggatga cttcgtccag
     1561 cagctggcgc ggcaggattt tctcgcggcg caggtagatc acgcgttcga cgatcagggc
     1621 caaggcgata atcgaggcaa tcagcaacag ccagatgggc cagccggcgg cttgaagaat
     1681 ggcgagcaaa aggaactcct ggatgggtgg aaagacgaga cgaaaacgaa ttgcgcaatg
     1741 taacgtgttg ccgactttgc ggcaagtaga gttctacaca ctttctgtgg ataaaagtgt
     1801 gcgcaagagc ccgtgcatcg ggaaagatcc ttgattccta agggaaattt ttccatgcgt
     1861 aaaaacttgg cagccagcgc aatagatttc caccttgcaa cttcgtcatc gtacaaatcc
     1921 ccgacttgcc cacaatttct gtggataaat ttgtgcggaa caacctttcc ttacggtaag
     1981 tctttgattt taattaaaga aatttccgcg atgaaaaaat tagcagtgtc cgtctgctcg
     2041 gccggcttaa cagtccagtc cgcttctgca cattttatgt ggacaaaatt gtgcgcaagc
     2101 gcacataaga aggcttaagc cattgattcg caacgacaaa gttgccgcgc ccgtttttta
     2161 ggcactgcgg gtgaacatca tgctcccctg ctttgcacac aaactgtgga caagaatgtg
     2221 cgcaagccca ccgcgaggac actcaagcga ttgaattcaa agggaataag ttcgcacgct
     2281 caaaatttga gcacgcccgg gaagggtaag gctggtatag tccgatccct gatgccaggc
     2341 cggcaagctg cggcggcggt gacgccggcg agcgatcgct tcagccaccc ggttcgaatg
     2401 ttcttcacaa atttcgtgga taagattgtg cgcaaggagc ttgatccgca tcaaaggtat
     2461 tgaatttaaa gggaaaattc tcactgcagg ttttttaggc atgcagctgt ttaagcagct
     2521 cgaagaccag cgccggatgc ccaggtttgc acacaatctg tggaaaacaa t
//
LOCUS       DASTPM010000631         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_426294, whole genome shotgun sequence.
ACCESSION   DASTPM010000631 DASTPM010000000
VERSION     DASTPM010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426294"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFR42_11130"
     CDS             <1..327
                     /locus_tag="VFR42_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369758.1"
                     /translation="VKMEMDLCWISVAGKDPIAYFNKYPGRFPLVHVKDMKVLPKGAE
                     GPTADPDKEMEDMTEVGGGVINWKHIFANSAKAGIEHYFVEHDFPKDAFASITKSYEY
                     LHNLTF"
     gene            401..1066
                     /locus_tag="VFR42_11135"
     CDS             401..1066
                     /locus_tag="VFR42_11135"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0369759.1"
                     /translation="MIRKLLTAVGCAGLCCVAMLARPQAKDWKPLFNGKDLTGWKHVG
                     PGGDTVEDGLIKTNGGMGLLYWAGGKVSNCRIRVVYKMRDFNDNSGVYIRIPIEPREP
                     WMPVHYGYEVQIDNHPETSNEDDTHITGTLYSLTKPLAKPGKPGPEWNTMIITLDGPH
                     TIVELNGVKVTDYTEGQPVPERKFDFEPLRGPRPNEGWFGLQNHSDNDIVFFKDISIQ
                     SLK"
     gene            1141..2487
                     /locus_tag="VFR42_11140"
     CDS             1141..2487
                     /locus_tag="VFR42_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0369760.1"
                     /translation="MKTTRRQFLHKTAGTAGALAVSRNILIEPEYIPTALQAALPASD
                     RLRFGIIGVGMQGSGLLANAIGLPGVECVAAADLYDGRHTLATEIVGKKIYTTRKYQE
                     LLDNKEIDCVIAAVPDHWHKQVVVDAVNAGKDIYCEKPMSHSPADGIAMVNAYKKTGR
                     VVQIGSQRVSSVVCAKAKELLAQGIIGDLNLVEGSLGRNDPCGAWEYPPPPDASPATI
                     DWDTWQGTVPKRAFDSKVFARWRCWKEYGTGLAGDLLVHLVSGMNFMLGWNEPPSRVL
                     SVGGILRWPDGRNMPDVHATLFEYGKYPVYLRLNAGCESPEIYRFQGSKGALEVTEFG
                     ITYSPQSGKDEYPCYYTSSYPAAMRAEYMKQWHEAHDATPGKEPLVESYTYKGNSWDD
                     VRPHLWNFFQHSRSHTPVVEDAVFGHHAALACHMANDSYFRKSAVTWDAASGTIKS"
     gene            2511..2759
                     /locus_tag="VFR42_11145"
     CDS             2511..2759
                     /locus_tag="VFR42_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369761.1"
                     /translation="MLRTVAKLSLGLAVLAVLAVSKPVAISQGAAKPPAARANVAIPP
                     REPRPEDVSSIDGMVKAYYEVVSGPAEKDREWGRDATL"
BASE COUNT          631 a          755 c          894 g          548 t
ORIGIN      
        1 gtgaagatgg aaatggattt gtgctggatc agtgtggctg ggaaggatcc aattgcgtat
       61 ttcaacaaat atccggggcg ctttcccctg gtgcacgtga aagacatgaa agtgctgccc
      121 aagggcgcgg aaggcccgac tgcggatccc gataaggaaa tggaagacat gacggaagtg
      181 ggcggcggcg tgatcaactg gaagcacatt ttcgctaatt cggcgaaagc aggcatcgag
      241 cattattttg tcgagcacga tttcccgaag gatgcattcg cgagcatcac caagagttac
      301 gaatatctgc acaatctgac cttctgatat ggacgaattg gtgcgcgcac cacgcgggct
      361 ggttaccgga cgcgagactc aacaaatttc aggaggccgc atgattcgca agttgctgac
      421 tgctgtgggc tgcgctgggt tgtgttgcgt ggcgatgctt gcccggccgc aggcgaaaga
      481 ttggaagccg ctattcaatg gcaaagacct gacgggatgg aagcacgttg ggccgggcgg
      541 cgacacggtg gaagacggct tgatcaagac gaacggcggg atggggctgc tctactgggc
      601 cggcggaaaa gtcagcaact gccgcatccg tgttgtctac aagatgcgag acttcaatga
      661 caactcgggc gtgtacatcc ggattccgat cgagccgcgc gagccgtgga tgccggtgca
      721 ctatggatat gaagtgcaga ttgacaacca tccggagact tcgaacgaag acgacacgca
      781 cattaccggc acgctgtatt cgttgaccaa gccgctagca aagcccggta agccggggcc
      841 ggagtggaac accatgatca tcacgctgga tggtccgcac accatcgtcg agctaaacgg
      901 cgtgaaagtt accgattaca ccgagggaca gccggttccg gaacgcaaat tcgattttga
      961 gccgctgcgc gggccgcgcc caaatgaggg ttggttcggg ctacagaatc acagcgataa
     1021 cgacatcgtt tttttcaagg acatcagcat tcagtccttg aagtaatttc tttggccgct
     1081 tggttggcag aacgctacag gcgcgtctaa taggaatgaa attttgcgga ggctggcacg
     1141 atgaaaacca ctcggcggca atttctacat aaaacggcgg gcacggcggg agcgctggca
     1201 gtttcgcgca atattctgat cgagccggag tacattccga cggcgctgca ggccgcgctg
     1261 ccggccagcg atcgtttgcg cttcgggatc atcggcgttg gcatgcaggg ctcgggactt
     1321 ctggccaatg cgatcgggct accgggcgtg gaatgcgttg cggcggctga tttgtatgat
     1381 gggcgccaca cgctggccac ggaaatcgtc ggcaagaaga tttacaccac gcgcaaatac
     1441 caggaactgc tcgacaacaa agaaattgat tgcgtcatcg cggcggttcc ggaccactgg
     1501 cacaagcagg tcgtagtgga cgcagtgaac gcgggcaaag acatttattg cgaaaaaccg
     1561 atgtcgcatt cgccggccga tggcattgcg atggtcaacg cgtacaagaa aaccggccga
     1621 gtggtgcaga tcggctcaca gcgcgtgagt tcagtggtct gcgcgaaggc caaagaattg
     1681 ctcgcgcaag gcatcatcgg cgacttgaac ctggtggaag gctcgcttgg ccgcaacgat
     1741 ccttgcggcg cgtgggaata tccgccgccg cccgatgcga gcccagctac gatcgactgg
     1801 gacacctggc aagggaccgt gccgaagcgg gcgttcgact cgaaagtctt tgcgcgctgg
     1861 cgctgctgga aggaatatgg cacaggcctg gcaggcgatc tgctggtaca cctggtcagt
     1921 ggcatgaatt ttatgctggg ctggaacgag ccgccgtcgc gcgtgctttc cgtcggcgga
     1981 atcttgcgct ggccggacgg gcgcaacatg ccggacgtgc acgcgacgtt gtttgagtac
     2041 ggaaagtatc cggtgtatct gcgcttgaac gcaggctgcg aatcgccgga gatttatcgc
     2101 ttccagggct ccaaaggtgc gttggaagtg acggagttcg gcatcaccta ttcgccgcaa
     2161 tcaggcaaag acgaatatcc gtgctactac acctcgagct atcccgctgc catgcgtgct
     2221 gagtacatga agcagtggca cgaggcgcac gatgccactc ccggcaaaga gccgctggtg
     2281 gaatcctaca cctacaaagg caattcgtgg gatgacgtgc ggccgcacct gtggaacttc
     2341 ttccagcatt cgcggtcgca cacgccggtc gtggaagatg ccgtattcgg ccaccacgcc
     2401 gcgctggctt gccacatggc aaatgattcg tacttccgca aatctgcggt tacctgggac
     2461 gcggcttccg ggaccatcaa gtcgtaactg caaaacagga ggacaacgcg atgctccgca
     2521 cggtagctaa gctgagcctg ggccttgctg tgcttgctgt tctcgcggtt tcgaagccgg
     2581 tcgcaatttc gcagggggcg gcaaagccgc cagcggcgcg cgccaacgtg gccataccgc
     2641 ctagggagcc gcgccccgaa gatgtctcct ccatcgatgg aatggtcaag gcgtattacg
     2701 aggtcgtctc cgggcccgcg gaaaaggatc gcgagtgggg acgcgacgcc acgctatagg
     2761 ttccgggaat ccggttcatc attttcagcc aaggcaagga cggcaagaca gtggcgcgat
     2821 cgctgtct
//
LOCUS       DASTPM010000632         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1262621, whole genome shotgun sequence.
ACCESSION   DASTPM010000632 DASTPM010000000
VERSION     DASTPM010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262621"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFR42_11150"
     CDS             <1..539
                     /locus_tag="VFR42_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369762.1"
                     /translation="TTLNHTYFMFGDYERALQASQQDFGYGTALTLALLDRTPEAIAM
                     LRQQEDSSPWRLGRLFLTSMRALLEGNREESLQASGELVRATFRDPEGLYYQARQLSY
                     LGADDWALEMFRRAVDHGFFCYQAFVRDPWLDRLRVKPAFPAIMQKASHLHQEALRIF
                     RSNVKDSLLGLAAENYDS"
     gene            627..1049
                     /locus_tag="VFR42_11155"
     CDS             627..1049
                     /locus_tag="VFR42_11155"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEU0369763.1"
                     /translation="MSASPVSVLEHNKQLTFRWFDDVWNQSRRDVIFERFAPNCVLHE
                     GHTPIRGPQEFADFYDRLQAQLSDIRITRGVALAQNDLVSLRWTVNAVHKATGKKTSV
                     SGISIVRIKDGRFVEAWQNWDAASMAAQLSDSPPAPLF"
     gene            complement(1273..1944)
                     /locus_tag="VFR42_11160"
     CDS             complement(1273..1944)
                     /locus_tag="VFR42_11160"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0369764.1"
                     /translation="MSTFTATERTQVKRLPKRGSYDCETVFKILDEAFVCHVGFVVDG
                     QPFVIPTNFGRSGETLYLHGSAASRMLRTLSEGVPVCVTVTLTDGLVLARSAFHHSVN
                     YRSVVVLGTARLVQDPAEKMEALRLFTEHIMKGRWNDIRRPTEQEMKGTTVLALPLEE
                     VSAKVRTGGPIDDEEDYSLPVWAGVLPLPVVPAEPIPDARLQAGTDAPAYMKGYRRGK
                     LPAME"
     gene            complement(2050..>3494)
                     /locus_tag="VFR42_11165"
     CDS             complement(2050..>3494)
                     /locus_tag="VFR42_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0369765.1"
                     /translation="HGGPSGHDADAWEDNWAYPNNLLTQRGAFLLKPNYHGSNNYGLK
                     WVESICCGKYYDLETPDINAGVDSLIAKGMVDPEKIATLGWSNGSILSTSLITTYPDR
                     YKVASVGAGDVEWISDWGNVMFGDSFDSYYFGKSPFEDPDLYIKKSPFFKMDKIKAPV
                     LIFHGTNDTNVPPAQSWSYFRALQYYDKVPVKFVVFPGEPHGPRKLTHQMRKVEEEVA
                     WFDKYFFKTNKLEDEAVKKDSRLEAELRRKNIARVSGGYYGTALKRSKKVDVLTVVPE
                     MVRRGDLQIGRFEVTRSQFAACQKTAQRQHTKAESASPTPAESDRYLLEINLPASGVS
                     LDEARQCAARLSELTGQQWRIPYEDEVKSLYEKRDGENTLDYWAGYAANPEDAAKLRE
                     AAKKLIGTAPLLKEVGSFHGQGKDDEEPVYDLGGNVAEWVLTRDGKGKVIGGSADCPA
                     DVKANCTPAPEYVGFRVVRGDAKPAKPDTH"
BASE COUNT          653 a         1186 c          859 g          796 t
ORIGIN      
        1 ccacgacgct caatcacacc tatttcatgt tcggcgacta cgaacgcgcc ctgcaagcct
       61 cgcagcagga cttcggctac ggaaccgcat taaccctggc cctgctcgat cgcactccgg
      121 aggccattgc catgctccgc cagcaggaag actccagccc ctggcgtctc ggcaggcttt
      181 ttctcacctc catgcgcgcc ttgctggaag ggaatcgcga ggagagtctt caggctagcg
      241 gggaactcgt gcgcgcaacg ttccgtgatc cggaaggcct gtactaccag gcgcgccaac
      301 tcagctatct gggcgccgac gactgggccc tcgaaatgtt tcgacgtgcc gtggaccacg
      361 gcttcttctg ctatcaggct ttcgtccgcg acccctggct ggaccggctc cgtgtcaaac
      421 ccgccttccc ggccatcatg caaaaggcct cccaccttca tcaggaagcc ctgcgtatct
      481 tccgctccaa tgtcaaagat tccttgctcg gcctcgccgc cgaaaattac gactcctagc
      541 ctttcgctgg ctcttctgac gcgctatagt cacgcgccgc ccgaccttgt atactcccgc
      601 caatcttgcc gcaggaggta ctcgttatgt ccgcctcgcc tgtttctgtc cttgaacaca
      661 ataaacagct caccttccgc tggtttgacg acgtttggaa ccaatcccgc cgcgacgtca
      721 tcttcgaacg cttcgctccc aattgcgttc ttcacgaagg ccacactccc atccgcggtc
      781 cacaggaatt cgccgacttc tacgaccgcc tgcaggctca gctctcggac attcgcatta
      841 cgcgaggtgt cgctctcgca caaaatgacc tcgtcagcct tcgctggacc gtcaacgccg
      901 tgcacaaggc caccggcaag aaaacttctg tatcgggcat ctccatcgtc cgcatcaaag
      961 acggccgttt cgtcgaagcc tggcaaaact gggacgccgc ttccatggcc gcccaacttt
     1021 ccgactcgcc gcccgcgccc ttgttctgag actttgttct gagaccattt gtcccagaca
     1081 gagctccgcc tgagttctcc ttatctagcc ttttagcttc tttagaatca atgcttccga
     1141 aaatcggggg gctggaccgg gagagatcaa tcagaaagtc gtcttcggat aactctttat
     1201 gtttcagata tttgctgcgg caacggggtg cactccaact tctgacccag tctgcggttg
     1261 acccaagcgg gcttattcca ttgccggaag cttaccccgc cgataaccct tcatgtacgc
     1321 cggagcatcg gtgcctgcct gcaaccgcgc gtcgggaatc ggctccgccg gcaccacggg
     1381 cagcggcagc actccagccc acaccggcag ggaatagtcc tcttcatcgt cgatcggccc
     1441 gcccgtccgc accttggccg acacttcttc cagcggcagc gccagcacgg tcgtgccttt
     1501 catttcttgc tcggtcggcc ggcggatgtc gttccagcgg cccttcatga tgtgctcggt
     1561 aaacaatctc agcgcttcca tcttctccgc gggatcttgc accagccggg ccgtgcccag
     1621 caccaccacc gaccggtaat tcaccgaatg atgaaacgcc gaccgcgcca gcaccagtcc
     1681 gtccgttagc gtcactgtca cgcacaccgg cacgccctcg ctcagcgtgc gcagcatccg
     1741 gctcgccgcc gatccgtgca ggtacagcgt ctcgccgctc cgcccaaagt tcgtcggaat
     1801 cacaaacggc tgcccgtcca ccacgaatcc cacatggcac acgaatgcct catccaaaat
     1861 cttgaaaacc gtctcgcagt catacgatcc gcgtttcggc aaccgcttca cctgcgtccg
     1921 ttccgtcgct gtaaatgtgc tcatcgttcg aggctatctg cggccctgca ctggcgcaag
     1981 agccaatctg gattgcctgg cactcacttg tcatgccgag cgaagcgagg aatctgcttt
     2041 tgcctttgct cagtgcgtat ccggcttggc cggcttcgcg tcgccgcgca ccacgcggaa
     2101 cccaacatac tccggcgccg gcgtgcagtt cgccttcacg tccgcggggc aatccgcgct
     2161 gccgccaatt actttgcctt tgccgtcgcg cgtaagcacc cactcggcca cgttgccgcc
     2221 caagtcgtaa accggctctt cgtcgtcttt gccctggcca tgaaaacttc ccacttcttt
     2281 tagcagcggc gccgtcccaa tcagcttctt cgccgcctcg cgcagcttcg ccgcatcctc
     2341 cggattcgcc gcataccccg cccaataatc cagcgtgttc tccccatccc gcttctcgta
     2401 gaggctcttc acctcatctt cataagggat ccgccattgc tgaccagtga gttcggatag
     2461 tcgagcggcg cactgcctgg cctcgtccaa cgaaacaccg gaagcgggca ggttgatttc
     2521 cagtagatac cgatcagact ccgctggggt cggggatgcg gattcagcct tagtgtgctg
     2581 tctttgtgcc gtcttttgac aggcagcaaa ctgagacctt gtaacctcga aacggccaat
     2641 ctgcaagtcg cctcgcctca ccatttcggg taccaccgtt aaaacgtcca ctttcttgga
     2701 ccgtttcagc gccgtgccat agtaaccacc cgagactcgt gcaatgttct ttctgcggag
     2761 ctctgcttct agtcgagagt ccttctttac cgcctcgtcc tccagtttgt tggttttgaa
     2821 gaaatatttg tcgaaccagg ccacttcttc ttccactttg cgcatctggt gtgtcagttt
     2881 gcgcgggccg tgcggctcgc cgggaaacac cacaaacttc accggcactt tgtcgtagta
     2941 ctgcagcgcg cggaagtacg accagctttg cgctggcggc acattggtgt catttgtgcc
     3001 gtgaaaaatc agcactggcg ccttgatctt gtccatcttg aaaaatggcg atttcttgat
     3061 gtacaggtcg ggatcctcaa acggcgactt gccgaaataa tacgaatcga acgaatcgcc
     3121 aaacatcacg ttgccccaat cgctgatcca ttccacgtcg cccgcgccca cgctcgccac
     3181 tttgtagcgg tcgggatacg tcgtaatcaa actcgtcgaa agaatcgatc cgttcgacca
     3241 gccgagcgtc gcgatttttt ccgggtccac catacctttc gcgatcagtg agtccacgcc
     3301 ggcattaatg tctggcgttt ccagatcgta gtattttccg cagcagatcg attccaccca
     3361 tttcaggccg tagttgttgc tgccgtgata gttcggcttc agcaaaaacg cgccgcgctg
     3421 cgtcagtaaa ttgttcggat acgcccagtt gtcttcccac gcatccgcgt cgtgcccgct
     3481 cggcccgcca tgaa
//
LOCUS       DASTPM010000633         5256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_455151, whole genome shotgun sequence.
ACCESSION   DASTPM010000633 DASTPM010000000
VERSION     DASTPM010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455151"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFR42_11170"
     CDS             <1..691
                     /locus_tag="VFR42_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0369766.1"
                     /translation="SFSYSVSHDLRAPLRGIDGFSQALEEDYAAHLDANARNYLGRIR
                     AGTQRMAMLIDDLLNLARVTRAELHRETIDLTKMASDIARDLQRAEPNRQITLKIDDS
                     LTANGDHRLVRVALQNLLGNAWKFTAKRADTRIEFSALGSNGRHVYFVRDNGAGFDQA
                     YAARLFGAFQRLHSVEEFPGTGIGLATVQRIIHRHGGKVWAEGAVNHGATVYFTLESE
                     HSIGGTEWNKV"
     gene            676..1125
                     /locus_tag="VFR42_11175"
     CDS             676..1125
                     /locus_tag="VFR42_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369767.1"
                     /translation="MEQSVILLVEDNPDDEALTLRALKKNNIRNEVIIARDGVEAVDY
                     LFGTGAHAGRDLSVMPQVILLDLKLPKMDGFEVLERIRSSEATQYLPVVILTTSSEDQ
                     DRIRGYDLGANSFVRKPVEFDKFIEAVRQLGLYWLILNETAPVPRRL"
     gene            1125..2699
                     /locus_tag="VFR42_11180"
     CDS             1125..2699
                     /locus_tag="VFR42_11180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0369768.1"
                     /translation="MPTPLRVLFIEDSEDDAALQVRLLRQAGYEVAFTRVQSADELRT
                     ALQSPWDIVISDYSMPHFSGTEALKVFRERGIDVPFIFVSGTIGEDAAVAALKVGAQD
                     YLMKTNLGRLSPSVQRELRESEERKQRKRLEEQVHQLQRFEAIGRLAGGVAHDFNNVI
                     GAIMGWAEIGAHVAAPGSEVHDKFLKIRSQADRASGLTRQLLAFARRQTLQPCNTNLN
                     ELARETISLLRNVIGERIEIHLELADDLHVIWADPGQVEQVLMNLSLNARDAMPSGGR
                     LIVETKNAVVDADYQRTHPYALPGNYVLLRVLDTGGGMDSETLAHIFEPFFTTKEIGR
                     GTGLGLATVYGIVKQHKGFIDVDTAPGKGTGFRVYLPLGNGPAQGTEKPAAFTMRGGS
                     ECILIAEDNDDLRDAAQEILQSLGYRVISTKDGVEALRIFEAEKNAIDLVFLDVVLPK
                     LNGPDAYLQMAELKPGLPVVFTTGYASEVSMVPIKTRKKATVLQKPYGSQYLAQKLRE
                     KLDRRRPRGLVSGPHS"
     gene            3103..>5256
                     /locus_tag="VFR42_11185"
     CDS             3103..>5256
                     /locus_tag="VFR42_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0369769.1"
                     /translation="MPSVLHGAIVLETKGDNLLKLRKVEITGFKSFCERTVVTFSGTG
                     TTCIVGPNGCGKSNVVDAISWVLGEQSHKSLRAGRMSDCIFNGTVKRPPMGLAEVTIT
                     LEDPELAEAARFVMESGPTEQPGADSATEASAPVEQNDILADVPAQPQPEISEEGASP
                     DAPLSDDSAAAGEEGKPEFLKGKRKKKSEKPVLVTKPGEVVVSRRLYRSGQSEYLING
                     RVARLRDIQDMFMGVGLGPDSYAIIEQGRIGQILSTKPMERRAIIEEAAGVTKFKTKK
                     RLAEAKLESSKVNLSRVNDIVVEVEKQLGSLKRQAAKARRYSEIREQMRGIVRQMLAG
                     KARELESEADRIAKQLEEFGATETQHAQAIQQREGEQDRLSYRNYELDTEIRQNQNQL
                     NLTALEADRAENKIAFNTQRSAELEGRRSQIVADLSAVSAQTTDWETRAASQKQAVAL
                     LREEATGLASRVIELQLQAQTRVAEISDSEARIEALRKAASHAGESLLRLHGEQKRAE
                     EALIHQGEALRKCEASEHDLLELSIRLRADAEDAAHVLEGAKSQLNALKQQGIELQSK
                     QHALRVEREERAKQSQTLRDSLAGVRARHATLTQILNDRSYTADAVQKLFAANERGGG
                     QDFRAVGVLADYAEVPELHEGAIEQYLRDELEYVVVETIDHARAGVSLLRDEVGGRAT
                     FFVDSTKNLRVDEYEPIIPFRMEDGVFARMDKLVEF"
BASE COUNT         1199 a         1642 c         1441 g          974 t
ORIGIN      
        1 atccttttct tattccgtct cgcacgatct ccgcgctccc ctgcgtggca tcgatggctt
       61 cagtcaggct ctcgaagagg attacgccgc ccatttggac gccaacgccc ggaattatct
      121 ggggcggatt cgggccggta cgcagcgcat ggccatgctg atcgacgacc tgctgaatct
      181 ggcccgtgtc acccgcgcag aactgcaccg ggaaaccatc gacttgacca aaatggccag
      241 cgatattgca cgagacttgc agcgcgccga accgaaccgg caaatcacgc taaaaatcga
      301 cgatagcctg acggccaatg gcgaccaccg cctggtgcga gttgccctgc aaaacctgct
      361 gggtaacgcc tggaagttca cggcaaagcg cgcggacacc cgcatcgaat tcagcgcctt
      421 gggctcgaac gggcgccacg tttactttgt gcgcgataac ggcgcgggct tcgaccaggc
      481 ttacgccgct cggctcttcg gcgcctttca gcgtcttcat tccgtggaag agtttccggg
      541 aacaggcatc ggcctcgcca ccgtccaacg cattattcac cggcacggtg gaaaagtttg
      601 ggcggaaggc gcagtaaatc acggcgccac ggtctatttc acgctggagt cagaacacag
      661 catcggaggg acggaatgga acaaagtgta atcctgctcg tggaagacaa tcccgatgac
      721 gaagctctga ccctgcgcgc cttgaagaag aacaacattc gcaatgaagt gatcatcgcc
      781 cgcgacggcg tcgaagccgt cgactacctc ttcggcactg gagcacatgc cgggcgagat
      841 ctttccgtta tgccgcaagt cattcttctg gacctgaaac tgccaaaaat ggatggcttc
      901 gaagtgctcg aacgcatccg ttcctccgaa gccacgcagt atttgccggt agtgattctt
      961 accacgtcca gtgaggatca ggatcgcatt cgcggctacg acttaggcgc caacagcttc
     1021 gtgcgcaagc ccgtcgagtt tgacaagttt attgaggcgg tccgtcagtt gggactgtac
     1081 tggctcatct tgaacgaaac ggccccggtc ccgcggagac tttagtgccc acgccacttc
     1141 gcgttctgtt catcgaagat tccgaagacg acgccgcgct gcaggtgcgt cttcttcgcc
     1201 aggccggtta cgaagtcgcc ttcacccggg ttcagtcggc cgacgaactg agaaccgccc
     1261 tgcaaagccc gtgggatatt gtcatttcgg actattccat gccgcatttc agcggcaccg
     1321 aagcattaaa agttttccgt gaaagaggaa tcgatgtccc tttcattttt gtttccggca
     1381 ccatcggcga agatgcagcg gtagcggcac tgaaagtcgg cgcgcaagac tatctgatga
     1441 aaaccaatct cggccggctg agtccgtcgg tgcagagaga gttgcgtgaa tccgaagaac
     1501 gcaagcaacg caaacgcctg gaagagcagg tccatcagct gcaacgcttc gaagcgatcg
     1561 gccgactggc aggcggagtg gcgcacgact tcaacaacgt catcggcgcc atcatgggct
     1621 gggcggaaat cggcgcccac gtcgcagcgc ccggcagcga agttcacgac aaatttctaa
     1681 aaattcgctc tcaagcggat cgcgcttcgg gactaacccg tcaactcctg gccttcgccc
     1741 gccgccagac tctgcagccc tgcaacacca acctgaacga actagccagg gaaaccatca
     1801 gcctgctccg caacgtcatt ggggaacgca ttgagattca cctggaactg gcggacgact
     1861 tgcacgtgat ttgggccgac ccgggccaag tcgaacaggt tctgatgaac ttgagtttaa
     1921 acgcgcggga tgccatgcct tccggcgggc gccttattgt cgaaacaaag aacgcagtcg
     1981 tcgatgcgga ctaccagcgc actcatcctt acgctctgcc gggaaattac gtcctcctgc
     2041 gcgtcctcga caccggcggc ggcatggata gcgaaacgct cgcgcacatc tttgagcctt
     2101 ttttcactac caaagaaatt ggccgcggca ccggacttgg actggccacc gtgtatggca
     2161 tcgttaaaca gcacaaaggc tttatcgatg tcgacaccgc tcccggaaaa ggcacggggt
     2221 ttcgcgtgta cttgccgctc ggaaacgggc cagcccaggg aacggaaaaa cccgccgcct
     2281 ttaccatgcg cggcggctcc gagtgcatac tcatcgcgga agacaacgac gacttgcgcg
     2341 atgcggctca ggaaatcctg cagagcctcg gctatcgcgt catttctacg aaagacggcg
     2401 tcgaagccct tcgcatcttc gaagcggaaa aaaatgccat cgacttggtc tttctggatg
     2461 ttgtgctgcc aaagctcaat gggcctgacg cctacctgca aatggccgag ctgaaaccag
     2521 gcctaccggt agtcttcacc accggctacg cgtccgaagt aagcatggtt cccatcaaaa
     2581 cacgcaagaa agcgaccgtg cttcaaaagc cttatggctc tcaatacctc gcacaaaaac
     2641 ttcgcgagaa attggaccgg cggcggccgc gcggtctagt cagcggacct cattcatgat
     2701 tgaacgttgg agcgcaggac gccgctcgcc ctgctcggtc tggaagtccg gtcattggga
     2761 actgcattac cgtcgcctcc gtccggccgc ctggaaatcg ccggcctaga acttcgctag
     2821 tggccgtgaa aatttcgcca caatcggttg cgtaggcctt tacaccaatc aggctccatg
     2881 ctacattttc ctcccgtacc gccatccgaa tcgtcacctg aaaaggtggc agcgccaggc
     2941 ggctgggcct ctgactgaga acctgccaaa cgtgtgtctg gtcaatgact tacaagtctt
     3001 gacggacggt tcgaaccgca ccgcggtccg agcatcgcga aaagaaaccc tccatcaggg
     3061 aacgatgagt cgtctctctt cgcgctgggt gtgtttcacc ccttgccgtc cgttttgcat
     3121 ggcgcgattg ttttagagac caaaggggac aacttgctaa aactccgcaa agtggaaatc
     3181 acgggattca agtcgttctg tgaacgaacc gtggttacgt tcagcggcac cggaacaacc
     3241 tgcattgtcg ggccaaatgg ctgcggcaaa tcgaacgtgg tggatgcgat cagctgggtc
     3301 ctcggcgaac agagccacaa gtccctccgc gccggccgca tgagcgactg catctttaac
     3361 ggcaccgtca agcgcccccc gatgggcctc gccgaagtca ccattactct cgaagacccc
     3421 gaactcgccg aagctgctcg cttcgtcatg gaatccggcc ccaccgaaca gccaggcgct
     3481 gattccgcga cggaagcatc agcgcccgtc gaacaaaacg acattctcgc ggacgtccct
     3541 gcgcagcccc agccggaaat ctccgaagag ggagcctcgc ccgacgctcc cctttcggac
     3601 gactctgcgg cagccggcga agaaggcaaa cctgaattcc tcaaaggcaa gcgcaagaaa
     3661 aaatccgaga agcccgtcct ggtcaccaaa cctggcgaag tcgtcgtcag ccgccgcctc
     3721 tatcggagcg gccagagcga atacctgatc aacggtcgcg ttgcccggct ccgcgatatt
     3781 caggacatgt tcatgggcgt cggcctcggt cccgactcct acgccatcat tgagcagggc
     3841 cgcatcggcc agatcctatc caccaagccg atggagcgcc gcgctatcat cgaggaagcc
     3901 gctggcgtca ccaaattcaa gaccaaaaag cgactggccg aagccaaact cgaatcctcc
     3961 aaagtgaatc tctcgcgcgt caatgacatc gtcgtcgaag tggaaaaaca gcttggctct
     4021 ctcaagcgtc aggccgcaaa ggcgcggcgg tactcggaaa ttcgcgaaca aatgcgcggc
     4081 atcgtccgcc agatgctggc cggcaaagcg cgcgaactgg aatcggaagc cgatcgcatc
     4141 gccaagcagc tggaagaatt cggtgccacg gaaactcagc acgcgcaagc cattcagcag
     4201 cgcgagggtg aacaggatcg cctcagctac cgcaactacg aactcgatac cgagattcgc
     4261 cagaatcaaa accagctgaa cctgacggcg ctcgaagccg atcgcgccga aaacaaaatt
     4321 gctttcaaca cccagcgctc cgctgaactc gaaggccgcc gctcccaaat cgtcgccgat
     4381 ctcagtgccg tttctgcgca aaccaccgac tgggaaacgc gcgcagcttc gcaaaaacag
     4441 gccgtcgccc tgctccgcga agaggccact ggtctggctt cgcgcgtgat cgaactgcag
     4501 cttcaggcgc aaacgcgcgt cgccgaaatt tctgactccg aagcccgcat cgaggccctc
     4561 cgcaaagccg cttcccatgc cggtgaaagc ctcttgcgtt tgcacggcga acaaaagcgg
     4621 gccgaagaag ctctgattca tcagggtgaa gcgctccgaa agtgcgaagc cagcgagcac
     4681 gacttgttgg aactttctat ccgcctgcgc gccgatgcgg aagatgccgc gcacgtcctg
     4741 gaaggtgcca agagccaact caacgcgctg aagcagcagg gcatcgaact tcagtccaag
     4801 cagcatgcac tccgcgtaga acgggaagag cgcgccaaac aaagccaaac gttgcgcgat
     4861 tcgctcgccg gtgttcgcgc gcgccacgcc acgctcacgc agattctcaa cgatcgttcc
     4921 tacacggcgg acgctgtgca gaaactcttc gcggccaacg aacgtggcgg cggccaggat
     4981 ttccgtgccg tcggcgttct cgccgactat gccgaggtac ccgagcttca cgaaggagct
     5041 attgaacaat acctgcgcga cgaactcgag tacgtggtcg tcgaaaccat tgaccacgca
     5101 cgcgctggcg tctctcttct gcgtgatgaa gtcggcggcc gtgccacctt cttcgtcgac
     5161 tccaccaaaa acctccgcgt cgacgaatac gagcccatca ttcccttccg catggaagac
     5221 ggcgtcttcg ctcgcatgga caagctggtt gaattc
//
LOCUS       DASTPM010000634         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_974044, whole genome shotgun sequence.
ACCESSION   DASTPM010000634 DASTPM010000000
VERSION     DASTPM010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974044"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VFR42_11190"
     CDS             complement(<1..977)
                     /locus_tag="VFR42_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369770.1"
                     /translation="MEVSQKPFARKKPDQLVYLELGSGNGGMLLSISEDGFRFRAVSP
                     LRPNGLMPFAFSFDGSRRLQGIGEVEWLEDDGKSGGMRFAEVSAEFRSAINQWLALDS
                     PRSRPAREVTPAAATPLDTMEKIREELRRGYSGPPARQLSTGKEAPAENPEVLLSKRD
                     TPPKKQDAEAEPRHPHIAADSDPRPELSTFGSSVAEARHDAAEFPGDPATTAAERLAS
                     EPPSRPLGTEQLRDDRKPRREHSPPPFSPPPFSQSVEEELPASAGPTNSAFLKKPAPA
                     SPVPSFRIPPVVPAASGPPQPVMDSHASAEALRRPFRSQPSMSRTERPY"
     gene            1554..3875
                     /locus_tag="VFR42_11195"
     CDS             1554..3875
                     /locus_tag="VFR42_11195"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU0369771.1"
                     /translation="MRTTLKLVLPLITSVALVSLLFAAYQVRKERRNLRNDLSRRAEL
                     LAESLQESIQPLFEKSERNSEKAMQRVVDRFAQREHMKGIAVYEANGNLSVMTSTLAD
                     SFKQMPGIVRTVLATDTGRGEFTQVADSPMYLYALPLERSDKAAGALLVIYEASFIDS
                     RVLLTMRDALLSALLETLLVSGLALVLVRWTFLDPLKRTSQWLRKLRAGETDTPESSL
                     PQGELFEQIQKEVKNLARDLGAARAAAAEEAQLREKQASVWTADRLRISLQSKLKGKP
                     LFVVANREPYMHVKGEKGIEVLVPASGLVTALEPVLEATDGTWVAHGSGSADRERVDE
                     TDRLRVPPDLPSYTLRRVWLTTEEVKGYYEGFANEGLWPLCHIAHTRPTFRPEDWLQY
                     QQANRKFADAVLQEMAGQEAPIVLVQDYHFALLPRMVKDARPDARVAIFWHIPWANPE
                     VFGICPWQRELVDGLLGADLIGFHTQTHCNNFLETVDRALEALTEWDRFAVNRQGHVT
                     RVRPYPISVAFPNGKPPREWRNTGEERARLCEELGIEASLLGVGVDRVDYTKGILERF
                     RGIERFIEMNPAYQRRFSFVQIGAPSRTDIPRYQQFLDEVTAEAERINARFQSGKWKP
                     IVLLRRHHSHQEIERFYRAASVCMVSSLHDGMNLVAKEFVVAREDEHGVLILSTFAGA
                     ALELTDALQVNPYDVQQVAAAILRALEMPDDEQAARIRRMRTTVKDHNVFRWAANLLS
                     DLTDIRIEPPERQENVVVSGEITTGDEQEEVRP"
     gene            3872..4567
                     /locus_tag="VFR42_11200"
     CDS             3872..4567
                     /locus_tag="VFR42_11200"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HEU0369772.1"
                     /translation="MSTGTSLAKAPFEIYTLAHVSRMGGPTANTLRELADGLETCSDE
                     SIYHHTIVAMRSHLVLADKLTNDFAQWARASLHKEDLADRLSRLDTTDYRTLGDLRNT
                     LSEIVRAYMEAFPKTADEATENPFYFCEGMEVVVPLELTARTLEEFRNCVREMSGESF
                     YLHFVAPRTRQEMQSNDFSVWLEKSLGLHELAAKINEIDVMDSTLEGAREKILQLLDL
                     GKEADLNITAAGN"
BASE COUNT         1051 a         1185 c         1506 g          901 t
ORIGIN      
        1 atatacgggc gctccgtgcg actcatcgac ggttgcgagc ggaacggtcg cctaagagct
       61 tcggcggaag cgtgcgagtc cattaccggt tgaggaggcc cggaagctgc aggtactacg
      121 ggcgggattc tgaaagacgg aaccggcgat gccggcgccg gctttttcag aaatgccgag
      181 ttcgtcggtc cggcacttgc cggtaattcc tcttcgacag attgactaaa cggtggcggg
      241 gaaaaaggtg gcggactgtg ctcgcggcgc ggttttcggt cgtcgcgcag ctgctccgtt
      301 ccgaggggtc gagaaggcgg ttccgacgcc agacgttccg cagcggtcgt ggcagggtca
      361 cccggaaact ctgccgcgtc gtgcctggct tcagcaacgc tgcttccgaa cgtggaaagt
      421 tccggccgcg gatcgctgtc agccgcgata tggggatgac gcggctccgc ttcagcatcc
      481 tgtttttttg gaggagtgtc acgtttcgaa agcagaacct ctggattctc ggcgggagct
      541 tctttgccag tggaaagttg gcgagcggga ggtcccgagt acccgcgccg aagttcctcg
      601 cggattttct ccatcgtgtc gagcggagtg gcggcggcgg gtgtcacttc gcgggcgggg
      661 cgagagcgcg gcgaatcgag agcaagccat tggttgatgg cagaacgaaa ttcggccgaa
      721 acttcggcga agcgcatgcc gccgctctta ccgtcgtctt cgagccactc aacttcgcca
      781 attccctgca ggcgacgact gccgtcaaaa gaaaacgcaa acggcatcag tccgttgggg
      841 cgcagcgggc tgacggcgcg gaaacggaaa ccatcttcag aaatgctgag gagcattcca
      901 ccgttgccgg aacctagttc gagataaacc agttgatccg gctttttgcg ggcaaaaggt
      961 ttttggctga cttccattgc aaaccttgca caattttaac ccgaaacgtg cggtttgccg
     1021 cccaaacaag gtggggactg gtgtttacct gtatagatca cggacgggag tgcatttttt
     1081 gcgcggccgg gtgagcgttg atgggagaaa gtacaggaat ctcccagtat ctgactcaat
     1141 ctagtagtgt tagaagtgta cggaggagca ggcggtggaa tcgagaatgg caccccagat
     1201 gccgcatctc ttcttgaaac caagcgaatt gggggccaga atgagactac aagttggatt
     1261 taccctggcg acgatttttc tggcgctgtt tacagcggcg ttgtggaagg agcgcagcca
     1321 gaaagagaga gccagaaaga gagagtaaga taagggagca atagtcgcgg gactatcgcc
     1381 ggatggttca tccggtaacc gcgacgtgat cacccaaaga acaatcgaag actcaagcgg
     1441 acggacgcgg cctatccctc aagagtaggc accacgaacg gcaagcctga ctgcaaaacc
     1501 cggcccgcgc gccgaagggg gaccccagac gcccctccac caaaggatag aaattgcgga
     1561 cgacgttgaa actcgttctg cctctgatca ccagtgtggc gctggtgtcc ctgttatttg
     1621 ccgcctacca ggtccgcaaa gaaaggcgaa atttaagaaa cgacctatcc cgccgcgcgg
     1681 aactgctcgc ggaaagctta caggaaagca tccagccact tttcgagaaa agcgaacgca
     1741 actcagagaa agctatgcag cgcgtggtgg atcgcttcgc tcagcgcgag cacatgaagg
     1801 gtatcgcggt gtatgaggcc aacggcaatc tgagcgtgat gacgtccacg ctggccgaca
     1861 gtttcaagca aatgcccggc atcgtgcgca ccgtgctggc gacagacacc gggcgcggag
     1921 agttcaccca ggttgcggat tcaccgatgt acctgtacgc attgccgtta gagcgcagcg
     1981 acaaagcggc cggggccttg ctcgtcattt acgaggcaag tttcattgat tcacgagtgc
     2041 tgctgacgat gcgagacgcg ctgttgagcg cgttgctgga gacgctgctg gtttccggcc
     2101 tggccctggt tctggttcgc tggacgttcc tcgatccttt gaagcgaacg tcgcaatggc
     2161 tgcggaaact gcgcgccggc gagacggata caccagagtc gtcgctgccg cagggagagt
     2221 tgttcgaaca aatccagaaa gaagtgaaga acctggctcg ggacttgggt gcagctcgtg
     2281 ccgcggcagc cgaagaagct caactgcgcg aaaagcaggc gtcggtgtgg acagccgacc
     2341 ggctccggat cagcctgcag agcaagctga agggcaagcc gctgtttgtg gtagcaaacc
     2401 gcgagccata catgcacgta aaaggcgaaa agggaattga agtgttggtg ccggcaagcg
     2461 gcctggtcac ggccctcgaa ccggtgctgg aagctaccga cggaacatgg gtggcgcacg
     2521 gatcgggcag tgcggatcgt gagagggtgg acgaaacaga tcggctgcgc gttccgccag
     2581 atctaccgag ctatacgttg cggcgtgttt ggctcacgac tgaagaggtg aagggctact
     2641 acgagggttt tgcgaacgaa ggattgtggc cgctttgtca catcgcgcat acacggccga
     2701 ccttccggcc ggaggactgg ttgcagtatc agcaggccaa tcgaaagttt gccgatgcgg
     2761 tgctgcagga aatggcgggt caggaagcgc cgatcgtact ggtgcaggat taccactttg
     2821 ctttgctgcc aagaatggtg aaggacgcga ggccagacgc gcgcgtggca attttctggc
     2881 atatcccgtg ggcaaatccg gaggtcttcg gcatatgccc gtggcaaagg gagctagtgg
     2941 acggcctgtt gggtgcggac ctgatcggtt tccacacgca aacgcactgc aacaattttc
     3001 tggagaccgt ggaccgcgcc ctggaagcgc tgacggaatg ggatcgcttt gcagtaaatc
     3061 ggcaaggaca tgtcacgcgg gtgcgcccct acccgatcag cgtagccttc ccgaacggga
     3121 agccgccccg ggaatggcga aacaccggag aagagcgggc gcgactgtgc gaagagttag
     3181 gcattgaggc ttcgctcttg ggcgtgggcg tcgaccgcgt ggattacaca aagggtattc
     3241 tggaaagatt tcgcgggatc gagcggttca tcgaaatgaa tcccgcttat cagcggagat
     3301 tctcttttgt gcagatcggc gcgcccagcc ggacggacat tcccagatat cagcaatttc
     3361 tggatgaggt gacggcggag gctgagcgga tcaacgcgcg tttccaatcc ggcaaatgga
     3421 agccgatcgt gttgttgagg cggcatcact cgcaccagga gattgagcgg ttctatcgcg
     3481 ccgcgtcggt gtgcatggtg agttcactgc acgacggcat gaatctggtg gccaaggagt
     3541 ttgtcgtcgc gcgcgaagac gaacacggag tgctgatttt aagtaccttc gccggagcgg
     3601 cgctggaatt gacggacgcg ctgcaggtga atccgtacga cgttcagcaa gtggcggcgg
     3661 cgattctgcg cgcgctggaa atgccggacg atgagcaagc cgcgcgaatt cgccgcatgc
     3721 gaaccacggt aaaggatcat aacgtcttcc ggtgggcggc aaatttgctt tcggatttga
     3781 ctgatattcg aatcgaaccg ccagaacgcc aggaaaacgt ggttgtcagc ggtgagatca
     3841 caacgggaga cgagcaggag gaagttcggc catgagcacc gggactagct tggcaaaggc
     3901 gccatttgag atttatacgt tggcgcacgt gtcgcgaatg ggcgggccga ctgcgaatac
     3961 gctccgcgag ctggccgatg gcttggagac ctgttcggat gaatccattt accaccacac
     4021 aatcgtcgcc atgagaagcc acctggtgct ggcggacaaa ctgacgaatg actttgcgca
     4081 atgggcgcga gcttcgctcc ataaagagga tcttgccgac cgtttatcca ggttggatac
     4141 cacggactac cggacgctcg gtgatttgag aaacactttg agcgaaattg tgcgcgcgta
     4201 tatggaggcc tttccgaaga cggcggatga agcaacggag aatccgtttt atttctgcga
     4261 gggaatggaa gtggtagtgc cgttggaact gacggcccgg acgctggaag aattccgcaa
     4321 ctgcgtgcgg gagatgagcg gcgagagctt ttacctgcac tttgtcgcac cgcggacacg
     4381 acaagagatg caaagcaacg atttttcagt gtggctggaa aagagtctgg gattgcacga
     4441 attggcagcc aagatcaacg agatcgatgt gatggacagc acgcttgaag gcgcgcggga
     4501 gaagattctg caattgctgg atctggggaa ggaagctgac ttgaacataa ccgcagcggg
     4561 aaattaagtt gcggacgggc ccgttcgagc gacgggatat tcatcagcgg tgcaaaagag
     4621 aaatggtaac atcgtgccgt agc
//
LOCUS       DASTPM010000635         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1031900, whole genome shotgun sequence.
ACCESSION   DASTPM010000635 DASTPM010000000
VERSION     DASTPM010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031900"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFR42_11205"
     CDS             complement(<1..1127)
                     /locus_tag="VFR42_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369773.1"
                     /translation="MITRREAVRLLGSTSAGALFGVPSGLYFPAAQQSSTSQLSVPPD
                     SPRWALEGEARVAEYLGRKCLFLDGGGATLRDFVLRDGVIDVDVLTPANRGFFGIQFR
                     ITDDNANAEWIYLRQHKSGLPDAMQYTPVLNTGLNWQIYNGPGFTGAVDIPRDSWFHL
                     RLEITGAQARLYVKDMQKPALVMNDLKSGVQKGQIALAVLIGATYWSNFEVRTTSDVP
                     WERHLPPMPAGTLTKWSISPAYDALARNVERPLTSSEGTAIRWQDVEAEPPGFVVLYR
                     YREAPHLNVTFANDFSKRLEPQPGTKLLYARTSIESDRDQVKKLYMGYSDEVSVFLNG
                     KILFRGRSAQNFRDPGFLGIVNPENDAVYLPLKKGSNELLLA"
     gene            1267..1758
                     /locus_tag="VFR42_11210"
     CDS             1267..1758
                     /locus_tag="VFR42_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369774.1"
                     /translation="MKPTGYLIAKLPNGLRGLPLCAALAILGLSGTALLSQQPPRQPQ
                     SQQWKTVPFAIVRYNDDAPKSWNVYHGEKKGIYLVRLWKRYLLINTADQSAYEIDPAK
                     VKVQGDGAELNPADVPSDPIETAEWKSRDSGPVVRHRFRFGQTGNFMELQIPLLANGK
                     PAY"
     gene            complement(1790..2398)
                     /locus_tag="VFR42_11215"
     CDS             complement(1790..2398)
                     /locus_tag="VFR42_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369775.1"
                     /translation="MTRCLDCGSVRDVDQCPACGLTSAAAELMFRKRLIKQTVFFLAG
                     SLLFPYVTPIYPPLDLDLMLVFFGVLFFVALTIAVFLDRRARKHQEIEILKRVYAGFI
                     PLPWILAAAIFLNGKMDSPGKVAYVPTQVQGKFLMKGLVRGSRRLVVRSWRPDQKFER
                     LPVDPDDFDRFKVGDRMEVAVEPGMLGIPWVYGVYREGRPRE"
     gene            complement(2415..2768)
                     /locus_tag="VFR42_11220"
     CDS             complement(2415..2768)
                     /locus_tag="VFR42_11220"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0369776.1"
                     /translation="MGTVQPNVRRLERRWAPRYSFRAGLEIEWGSAVLRASTRDVSAS
                     GMFIESADPLWIGAGFSANLQLDHPVRVDCRVRRVVPGHGMGVSVSLTKEQQQTYQQL
                     LSELSQSAPEAEQAG"
BASE COUNT          661 a          949 c          763 g          546 t
ORIGIN      
        1 gccagcagca gttcattgct tccctttttc agcggcaaat acaccgcgtc attttccggg
       61 ttgacgatcc ccaaaaaccc cggatcgcga aaattctgcg cgctgcgccc acgaaacagg
      121 atttttccgt taagaaacac gctgacctca tcgctataac ccatatatag cttcttgacc
      181 tgatcgcggt cggactcgat gctcgtccgc gcatacagaa gcttggtgcc gggctgcggc
      241 tccaaacgtt tcgaaaagtc attggcaaac gtgacgttca aatgcggtgc ctcgcggtag
      301 cgatagagca ccacaaaacc aggcggctcc gcttccacgt cctgccaccg gatggctgta
      361 ccttccgagc tagtaagagg ccgctcaacg ttgcgggcca aagcatcgta ggcaggtgaa
      421 atgctccatt tggtcaaagt gccggccggc atcggcggca agtggcgctc ccatggaaca
      481 tccgacgtag tccgcacctc gaagttcgac caatacgtcg cgccaatgag caccgccaac
      541 gcgatttggc ctttttgcac gccgcttttc agatcgttca tgaccaacgc gggcttttgc
      601 atatctttca cgtaaagtct tgcttgtgcg ccggtgattt ccagccgcag gtggaaccag
      661 ctgtctctcg gaatatccac cgcgccggtg aaacccggcc cattgtaaat ttgccagttc
      721 aaaccggtgt tcagcaccgg cgtgtactgc attgcgtcag gaagtccgga tttgtgctgg
      781 cgcaggtaaa tccactccgc gttggcgttg tcgtctgtaa tccgaaactg gatgccgaag
      841 aatccgcgat tcgcgggcgt tagaacgtcc acatcaatca cgccgtcacg cagcacaaag
      901 tctctcagcg tcgcccctcc gccatccaga aataggcatt tgcggcctag atactcggcc
      961 acccgagctt ctccttccag cgcccagcgc ggcgaatccg gtggtacgga aagctgcgat
     1021 gtcgagcttt gctgagccgc cgggaaatac aggccggaag gcactccgaa caatgccccg
     1081 gccgaagtcg aacccaacag acgcaccgct tctcgtcgag taatcatcgg agcagctacc
     1141 tcatgttttc tgtcttgatg gtcccaaggc ttgcaaatgt agacgacgcg cacgaagaat
     1201 tggtagcagg actgaggcca atcaacgccg aatcacgccc tggtttatct tgtaaaatag
     1261 ttgggaatga aaccgaccgg ctatctcatc gccaagttgc ccaacggatt gcggggcctc
     1321 ccgctctgcg cagcgctagc gattctcgga ttatccggaa ccgcgctcct ctcccagcag
     1381 ccaccgcgcc agccgcaaag ccagcaatgg aagaccgtgc cgtttgccat cgttcgctac
     1441 aacgacgacg cccccaaatc ctggaacgtt tatcacggcg aaaaaaaagg catctacctg
     1501 gtccggctct ggaagcgcta cctgctgatc aacacggccg accagagcgc ctatgaaatc
     1561 gatcctgcta aagtaaaagt tcagggcgac ggcgccgagc tgaatccggc agacgtcccg
     1621 agcgatccga ttgaaaccgc cgagtggaag tcgcgcgact ccggcccggt ggtgcgccac
     1681 cgttttcgct tcgggcagac gggcaatttt atggaattgc agatcccgct gctcgccaac
     1741 ggcaagcccg cctactgaac caccggccgc gcttcacagg tcacgtgcgt tactcgcgag
     1801 gccgcccctc gcgatataca ccgtacaccc agggaattcc cagcatgccc ggctcaaccg
     1861 ccacttccat ccgatcgccg accttaaacc ggtcgaagtc atctggatcc accggcaatc
     1921 gttcgaattt ctggtctggc cgccacgacc gcaccaccaa tcgcctgctc ccgcgcacca
     1981 gccccttcat caaaaacttc ccctgcacct gcgtcggcac gtacgccact ttcccaggcg
     2041 aatccatctt gccgttcaga aaaatcgccg ccgccagaat ccacggcaac ggaataaatc
     2101 cggcgtaaac gcgcttcaaa atttcgattt cctgatgttt gcgggcgcgg cgatcgagaa
     2161 acacggcaat cgtcagcgcg acaaaaaaca gcacgccgaa aaacaccagc atcaaatcca
     2221 gatccagcgg cggataaatc ggagtgacgt agggaaaaag cagtgaaccc gccagaaaaa
     2281 ataccgtttg cttgatcagg cgcttgcgga acatcagttc cgccgcggcc gacgtcagcc
     2341 cgcacgccgg acactggtcc acatcccgca cggagccaca atccaggcaa cgggtcatgc
     2401 agctgagtat accgtcagcc cgcctgttcc gcctcgggcg ccgactgcga aagctcgctg
     2461 agtaattgct ggtaggtctg ctgctgctct ttcgtcaacg aaaccgagac gcccatccca
     2521 tggcccggca ctacgcgccg tactcggcaa tccacgcgca ccgggtgatc gagctgcaaa
     2581 tttgcggaga agcccgcgcc aatccacagc ggatcggccg attcgatgaa cataccactg
     2641 gcgctgacgt cgcgcgtgct ggcccgcagc accgccgagc cccattcgat ctccaggccc
     2701 gcgcggaaag aataacgcgg cgcccagcgg cgctcgaggc gccgaacatt tggttgtacc
     2761 gtgcccatga agctggtgcc ccctgcgtgg gttaccgagc tgcggcgcat gggcgttcac
     2821 gccgctccca acagattcag atccgcagca taccagctcc gaatgaacgt aatgtgaaaa
     2881 gcatacgccg cctgcaagca caacgatacg gcaccgcag
//
LOCUS       DASTPM010000636         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1291254, whole genome shotgun sequence.
ACCESSION   DASTPM010000636 DASTPM010000000
VERSION     DASTPM010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1291254"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VFR42_11225"
     CDS             <1..1390
                     /locus_tag="VFR42_11225"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU0369777.1"
                     /translation="ELEQLNLKAKEQRKNRVAAHKVQEERIKYLTDEKRIKAVTVLFS
                     DLEGRLHMLDYDKKFLIKSWDNLTFDGSSIRGFTAQRESDLRLGMDWSAFYWAPADFF
                     GPGKVLVFGEVIDKDGSPYSADIRGALKLYADQLHKKEGYTLNAANEIEGFLFKGVDA
                     ERHFHENGKFEYVNTGGYYHSLPGDLLRSFIDTTAEVQRAMGFQNEKDHPEVAPSQFE
                     INYGYGEVVAAADQIQLYKLICRQVATHMGLTASFLPKPVVGVNGSGMHTNVSISENG
                     KNLFWDPKGEEKLSKLGWSFVDRILTHGNDICLLLNASVNAYRRLDPHFEAPNQIKAS
                     PVDRGSMIRIPIGNEKSMRVEVRSVAPDANPYLVMYSIFKTGIDGDTAKIKNLRQAER
                     YLPDNIYTAIENLEKADWTTKLMGADVKSRYADLKRASADRCPRALGTFVKAQEVQYH
                     HEVYNQFLWNLF"
     gene            1895..>2487
                     /locus_tag="VFR42_11230"
     CDS             1895..>2487
                     /locus_tag="VFR42_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0369778.1"
                     /translation="MPELTPDLIVIGFIWYIVFLFSTTCHEASHALAAKLGGDDTATL
                     GGQLTLNPVPHIRREPWGMVLVPLLAFLFTRQIIGWASAPFNPEWERRYPKRSAWMAL
                     AGPAANFTLMLIAVVMIHLARHFGWFYRGPDGKAGFFELIVSAFYFLNLLLGVFNLLP
                     VPPLDGSTGIMLFMGEGTARSYLDWLRTSPYRLVGLL"
BASE COUNT          536 a          666 c          768 g          517 t
ORIGIN      
        1 cgaactggaa caactcaacc tgaaggccaa agagcagcgc aagaatcgcg tggctgcgca
       61 caaggtccag gaagagcgga tcaagtacct caccgacgaa aagcgcatca aggcggttac
      121 ggttttgttc agcgacctgg aaggccggct gcacatgctg gactacgaca aaaagttttt
      181 aatcaagagc tgggacaacc tgacgtttga cggctcgtcg atacgcggat ttacggcgca
      241 gcgggaaagc gacttgcgac tgggcatgga ctggagcgcg ttttactggg cgccggcgga
      301 cttttttgga ccgggcaagg tgctggtgtt cggcgaagtg attgacaagg atggttcacc
      361 gtattcggcg gacattcgcg gagcgttgaa actttacgct gatcagctcc acaagaagga
      421 aggctacaca ctcaacgccg cgaacgagat tgaaggattc ttgttcaagg gagtagatgc
      481 cgagcggcac ttccacgaga acggaaaatt cgagtacgtc aacacaggcg gctactatca
      541 ttcgctgccc ggcgacctgt tgcgcagctt cattgacacg acggcggaag tgcagcgggc
      601 gatgggcttc cagaacgaaa aggatcaccc ggaagtagcg ccttcgcaat ttgagatcaa
      661 ctacggatat ggcgaagtgg tggcggcggc ggaccagatt cagctctata aactaatttg
      721 ccggcaagtg gcaacgcaca tggggctgac cgcgtcgttt ttgcctaaac cggtggtcgg
      781 cgtgaacggc agcggcatgc acaccaacgt ttccatcagc gaaaacggaa agaacctgtt
      841 ctgggatccc aagggcgagg aaaaactgag caagctgggc tggtcgttcg tggaccgtat
      901 cctgacgcac ggcaacgata tttgtttgct gctgaacgcc agcgtcaacg cctatcgccg
      961 gctggatccg catttcgagg caccgaacca gatcaaagca tcgccagtcg atcgtggctc
     1021 catgatccgc atccccattg gcaacgagaa aagcatgcga gtcgaggttc ggtcggtggc
     1081 cccggacgcg aatccttatc ttgtgatgta ttcgattttc aaaaccggaa tcgacggcga
     1141 cacggcgaag attaagaacc tgcgacaagc cgagcgctac ctgcccgaca acatttacac
     1201 cgcgatcgaa aacctcgaga aggcggactg gacgaccaag ctgatgggcg cggatgtgaa
     1261 gagccgctac gccgatttga agcgtgcttc cgccgaccgg tgcccgcgcg cgctgggcac
     1321 cttcgtgaaa gcgcaggaag tgcaatatca ccacgaggtg tacaaccagt tcctgtggaa
     1381 cctgttctag gctccggtca accgctgcgg tttaggaaac gccctgttgg agcgttcttt
     1441 ttcattcact cgggcctgtc gtaccgaact tggcagctcc acaaggagag cgccgcgcgg
     1501 aatccgtcgg cgccgggtgt aggttctggc tggcggtatg agggttcgcc gtggcggtgt
     1561 ttcgggagga cttggcaaaa tctcctggtg gattcccttc gtcgccgtcc tggcgcgctg
     1621 cgcattcggc gtcggcactc ggtgccgcct gcgagcaagt gcagaaccgt gccaatcacg
     1681 gatgccgcag ggccgcaaaa tcaatcagtt ggaaacgcgg acgcgccagg agtcaggccg
     1741 ggagtgagtc catgctggaa catttggaga tcccgctatg gaaagcgagg aaatctccgg
     1801 cgaggcaaca gcagtgcggt acgcagccgc agccgcttgc attcgcaatg ttctgcccct
     1861 actctggaga cgccgcgttt tctgcgaaca aaaaatgcct gagcttacgc ccgacttaat
     1921 cgtcattggc tttatctggt atatcgtctt tctgttttcg acgacgtgcc acgaagcctc
     1981 gcatgcgctc gccgcgaaac tgggcgggga cgacaccgcg acgctgggcg ggcagttgac
     2041 gctgaatcca gttccacaca tacgtcgcga gccatggggc atggtattgg tgccactgct
     2101 ggcgtttttg tttacgcgcc agatcatcgg ttgggccagc gctcccttca atccggagtg
     2161 ggagcggcgg tatccgaagc gttccgcatg gatggctttg gccggacctg cggccaattt
     2221 cacgctgatg ctcattgcgg tcgtgatgat tcatctggcc agacacttcg gctggtttta
     2281 ccgcggcccg gatgggaaag cgggattttt tgagcttatc gtgagcgctt tttatttcct
     2341 gaatctgttg ctgggggtat tcaatctgtt gccggtacct ccgctggatg gcagcacggg
     2401 catcatgttg tttatggggg aggggacggc gcgaagctat ctggactggc tgcgcacgtc
     2461 gccataccgg ctggtaggct tgctgat
//
LOCUS       DASTPM010000637         2670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1205120, whole genome shotgun sequence.
ACCESSION   DASTPM010000637 DASTPM010000000
VERSION     DASTPM010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205120"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFR42_11235"
     CDS             complement(<1..603)
                     /locus_tag="VFR42_11235"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HEU0369779.1"
                     /translation="MNFHWFSRKQRTDELNEELESHLTMATEDLVHRSESRTAAALAA
                     QREMGNLLLIREVTQDMWGGRWWRDLLEDIQYGLRVLWKNPRFLTVAVLTLALGIGAN
                     TALFSVVNGVLLNPLPYPHPEELVTLHESKPNFSTGSISFPNFLDWQRDNQTFSSMAV
                     QRGTSFILSGLGEAEQIDAQFVSSDFFRQLGVVPAIGRDFA"
     gene            complement(600..962)
                     /locus_tag="VFR42_11240"
     CDS             complement(600..962)
                     /locus_tag="VFR42_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU0369780.1"
                     /translation="MAKQQYRNRIELLQGTLDLMILQTLQWGPQHGYGISQAIRNRSG
                     EILQVDTGSLYPALHRLEKQKWVASEWKVSGNKQRAKYYRLTAAGKRQLAQERSRWQQ
                     LTEAIGGVLDQAGNGVES"
     gene            1095..2417
                     /gene="purB"
                     /locus_tag="VFR42_11245"
     CDS             1095..2417
                     /gene="purB"
                     /locus_tag="VFR42_11245"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU0369781.1"
                     /translation="MIPRYTREEMGRVWSDSNKYSKWLEVELAATETLAEAGQVPGEA
                     AATIRSRARVDAARINELEAQVKHDVIAFTMNVGESIGDPAAARWLHYGMTSNDVVDT
                     AQALLVRDASRLIEAALARFSDVLARRAQEFRHTPQIGRTHGVHAEPITFGLKVANWY
                     AENQRNIERFADAAAQMRIGKISGAVGNASHLGPEIEERICQRLGLNVAPVASQVIQR
                     DRHAHYVSTLALIAATLEKIAIEIRHLQRTEVREAEEPFGAEQRGSSAMPHKRNPVIC
                     EQISGLARIVRSNMIAAYENIGLWHERDISHSSVERIILPDSTILIDYMLAKMTTVVA
                     DMRVFPERMRKNLDATQGLVFSGQLLQDLVEKGMPRDEAYKTVQENAMAAWETDASFR
                     ERVGKDPRVTKYLDAKALEHSFELQRHLRYVDAIFARVFGEKTTATKA"
BASE COUNT          572 a          816 c          747 g          535 t
ORIGIN      
        1 ggcgaagtcc cgcccgatgg ccggaacaac gcccagttgc cggaaaaaat ccgaactcac
       61 aaactgcgcg tcgatttgtt cggcctcacc taggccgcta aggataaagg aggtgccgcg
      121 ctgcaccgcc atggaagaaa aagtctggtt gtctctctgc cagtccagaa aattgggaaa
      181 tgaaatggag ccggtcgaaa agttgggctt gctctcgtgc agcgtaacca gctcttccgg
      241 atgaggataa ggcagaggat tcagcagaac tccattcacg acggaaaaca gggccgtgtt
      301 cgccccgatc cccagggcaa gcgtcaggac ggcgaccgtg aggaaacgcg gattcttcca
      361 aagcacgcgc aaaccgtact ggatgtcttc cagcaggtct cgccaccagc gtcctcccca
      421 catgtcctgg gtcacctctc gaatcaacag cagattcccc atttcgcgct gcgcggctaa
      481 cgctgcggca gttcgcgatt cactgcggtg gactaggtct tcggtcgcca tggtcagatg
      541 cgattcgagc tcttcgttca actcgtccgt tcgctgcttc cgactaaacc agtggaagtt
      601 catgactcca ccccattccc ggcttggtcc agcactccgc cgattgcttc ggtcagctgc
      661 tgccagcgtg aacgttcctg cgccagttgc cgttttcccg ccgcggtcag gcggtagtat
      721 ttggcgcgct gtttgttgcc ggaaaccttc cactcagaag cgacccactt ctgcttttcc
      781 aggcggtgca gcgccggata gagcgagcca gtatcgactt gcagtatttc gccggagcgg
      841 ttccggatgg cctggctgat tccatagcca tgttgcggac cccattgcag cgtctgcagg
      901 atcatcagat ccagggttcc ctgcagaagc tcaatccggt ttcgatattg ttgtttggcc
      961 atagccgttc atccccgcaa atgttagaga tgtagagctc ctacatccat ggatgtagag
     1021 tctctacctc ctacaatcag attgtcaaga tgcgatatga tggtgctgat ctaccagaaa
     1081 aagagaggat tcgcttgatt ccacgctata cccgcgaaga gatgggccgc gtctggagcg
     1141 actccaacaa atactccaag tggctggagg tcgagctggc ggccacggag acgctggctg
     1201 aagccggaca ggttccgggc gaagctgccg cgacgattcg ttcgcgcgca agggtggacg
     1261 ccgcgcgcat taacgaactg gaagcccaag ttaagcacga cgttatcgcc ttcaccatga
     1321 atgtgggcga atccatcggc gatcccgccg cggcgcgctg gctgcactac ggcatgactt
     1381 cgaacgacgt ggtggacacc gcgcaggcgc tactggttcg tgatgcgtcg cgactgattg
     1441 aagccgcgct cgccaggttc agcgatgtgc tggcgcggcg cgcgcaggag tttcgccaca
     1501 cgccgcaaat tggccgcacg cacggtgtgc acgccgagcc catcactttc ggattgaaag
     1561 ttgccaactg gtacgcggaa aatcagcgca atatcgaacg cttcgcggat gctgccgcgc
     1621 agatgcgcat cggcaagatt tccggtgcgg tcgggaatgc ttcacacctg ggcccggaaa
     1681 tcgaagagcg catttgccag cggctcggcc tcaacgttgc gccggtggct tcacaagtga
     1741 ttcagcgcga ccgacatgcc cactacgtga gcacgctggc gttgattgcc gccacgctgg
     1801 aaaaaatcgc catcgagatt cgccatttgc agcgcactga agtccgcgaa gctgaagagc
     1861 cgtttggtgc cgaacagcgg ggtagttcgg cgatgccgca caagcgtaat ccggtcatct
     1921 gcgagcagat cagcggcctg gcgcgcatcg ttcgctcaaa catgattgcc gcttacgaaa
     1981 acatcggcct gtggcacgaa cgcgatattt cccacagctc agtcgagcgc atcatcctgc
     2041 ccgattccac cattctgatc gactacatgc tggccaaaat gaccacggtg gtcgcggaca
     2101 tgcgtgtttt tcccgaacga atgaggaaga atctcgacgc cacgcagggc ctcgtcttct
     2161 ccggccaact cctgcaggac ctggttgaaa aaggcatgcc ccgcgacgag gcctacaaaa
     2221 ccgtccagga aaacgccatg gctgcctggg aaaccgatgc cagcttccgc gaacgcgtag
     2281 gcaaggatcc ccgcgtcacc aaatatctcg acgccaaggc tctcgaacac agcttcgagc
     2341 tccagcgcca cctgcgctac gtcgacgcca tcttcgcccg cgtcttcggc gaaaagacca
     2401 ccgccacaaa agcctgaagg tggtgtctgc aaaaacagca gcgccccaat cgcgttcacg
     2461 atggggcgcc tttttcacct cttctgaatc cgacttgcgc ccgattcagc tgcggaggcg
     2521 gaaagtcaac tattccacgc gctgcacgta gcgcgcttcc gaagtgtcca ctcgaatctt
     2581 atcaccttcg ttcacgaacg ggggcaccgg aaccaccagt cctgtttcca aagttgccgg
     2641 cttcatcacc gagctggctg tagctttctg
//
LOCUS       DASTPM010000638         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_109271, whole genome shotgun sequence.
ACCESSION   DASTPM010000638 DASTPM010000000
VERSION     DASTPM010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109271"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..907)
                     /gene="leuD"
                     /locus_tag="VFR42_11250"
     CDS             complement(263..907)
                     /gene="leuD"
                     /locus_tag="VFR42_11250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0369782.1"
                     /translation="MKPFSAHTGLVLPLDRVNVDTDQMVPKQFLKALTREGFGRILFY
                     DWRYKLPTETPNPEFIMNWPEYKGASVLLTRANFGCGSSREHAPWAIGDYGFRVILAP
                     SYADIFYNNCFKNGILPAILKESEIDELFKRAEADRAYQLNVDLQSQAVTDNKGLKFT
                     FEIAPSRKEVLLKGLDDIGTTLQHAAAIDAYEKSHTGTATMFAPVDVKYYSKAD"
     gene            complement(926..2347)
                     /gene="leuC"
                     /locus_tag="VFR42_11255"
     CDS             complement(926..2347)
                     /gene="leuC"
                     /locus_tag="VFR42_11255"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU0369783.1"
                     /translation="MAAKTLYEKIWNNHVVYEEQGKPSLLYIDRHLIHEGTSPQAFSG
                     LRAERRKVRRPDLTFAVMDHSVPTTDRTLPIIDMDAKAQFEALAKNCQETGVRLFDMN
                     SKNQGIVHIIGPELGITQPGQTIVCGDSHTSTHGAFGTLAFGIGTSEVEHVLATQCLV
                     QGKSKTLHILVHGKRPKGVTAKDIILAIIGRIGIGGGTGHVAEYAGEAIRDLSMDGRM
                     TVCNMTIEGGARAGMVAPDETTFAYLEGRPFVPRGKEFQEAADRWKLLKSDDGAKFDT
                     TVELHAEKIAPQVTWGTNPGMVTGVTDRVPDPGSFQNPDDQKATERALNYMGLKAGTP
                     IVDIPVDRVFIGSCTNSRLDDLRAAAQLVAGKHVAKIIKQALVVPGSRGIKAAAEKEG
                     LDKVFSEAGFEWRDAGCSMCIGMNADVLPPHERSASTSNRNFEGRQGRDSRTHLVSPV
                     MAAAAAIAGHFVDVREFDVAHVE"
     gene            2497..3225
                     /locus_tag="VFR42_11260"
     CDS             2497..3225
                     /locus_tag="VFR42_11260"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEU0369784.1"
                     /translation="MKRRAAGMLLWLAVVFAGCRVSRQSTITHGELVRRTQEIMDATA
                     PGIPDPWQKYFAEDAMYFDEKGRAMDKAALLKDVSPLPKGFSGTIKVLNARSNILRDT
                     AVLSYDLDETEIVYGQELKARYHGTDTWMERNGQWQIIAGQMLRYYEDPAPGASNSAD
                     HKSYAGTYELAPGITRTVSTRGQDLMVQRSGRAAETLIPEAQGIFFRKGVEGRILFRR
                     DAAGKVDALIDRRNNEDIVWKRIS"
     gene            3270..>3517
                     /locus_tag="VFR42_11265"
     CDS             3270..>3517
                     /locus_tag="VFR42_11265"
                     /inference="COORDINATES: protein motif:HMM:NF014870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine methyltransferase"
                     /protein_id="HEU0369785.1"
                     /translation="MDTISYTWYESPVGPLLLAGSHAALRYVRFSAGKHAVAIEPRWT
                     ENRNMFSEAIRQLSEYFAGERKTFTLELGPPGTEFQRSV"
BASE COUNT          682 a          885 c         1168 g          782 t
ORIGIN      
        1 cccctattgt cgtggaagtg gttattcttg aagtcccttg ttttcatata ctttcaaggt
       61 cttgattcta agcgagttag ggggcaggtt gggtggcaga aaccggatgc cacccttccg
      121 acttttgtca ggtcaggtgc cgggtgggcg ctaggttaat ttagcggcga ttggacgagc
      181 atccgagcgt ggttatctat gacgataagg ctacttattt tagactgccg cgtcaaggga
      241 tcgccggctg cggtgcttaa ggctaatcgg ccttggagta gtacttgacg tccacggggg
      301 cgaacatagt ggccgtgccg gtgtgggatt tttcgtaggc gtcgatggcg gcggcgtgtt
      361 gcagggtggt gccaatgtcg tcgagccctt tgagcaggac ttccttgcgt gacggcgcga
      421 tctcaaacgt gaatttcaga cctttgttgt cggtgacggc ctggctctgc aagtcgacgt
      481 tcagctgata ggcgcgatcg gcttcggcgc gcttgaagag ttcatcgatt tcagattcct
      541 tcaagattgc cggaaggatg ccgttcttga agcagttgtt atagaaaatg tcggcgtagc
      601 tgggcgccag gatgacgcga aaaccgtagt cgccgatggc ccagggggcg tgttcgcggc
      661 tggagccgca gccgaagttg gcgcgggtca gcagaacgga ggcgcctttg tactccggcc
      721 agttcatgat gaattcggga ttgggcgttt cggttgggag tttgtagcgc caatcgtaaa
      781 aaagaatgcg gccaaagcct tcgcgggtga gggctttgag gaattgcttg ggcaccatct
      841 ggtcggtgtc gacgttgacg cgatcgagcg gcagaacaag tccggtgtga gcgctgaagg
      901 gtttcatagt ttcggatcct tgagattatt ccacgtgcgc gacgtcgaat tcgcgcacgt
      961 cgacgaagtg ccctgcgatg gcggcggcgg cagccatcac ggggctgacc aggtgcgtgc
     1021 ggctatcacg gccttggcgg ccttcaaaat tgcgattgga cgtgctggcg ctacgttcgt
     1081 gcggaggcaa aacgtcggca ttcatgccga tgcacatgct gcagccggcg tcgcgccact
     1141 caaagccggc ttcggagaat actttgtcga ggccttcttt ttccgcggcg gccttgattc
     1201 cgcgggaacc gggaactacc agcgcctgct tgatgatttt tgcgacgtgt ttgccggcga
     1261 cgagttgcgc tgcggcgcgc aggtcgtcca agcgggaatt ggtgcaggag ccgatgaaga
     1321 cgcggtcgac gggaatatcc acgatgggcg tgccggcttt gagtcccatg tagttcaacg
     1381 cgcgttcggt ggctttctgg tcgtcgggat tctggaacga gccgggatcc gggacgcgat
     1441 cggtgacgcc ggtgaccata cccggattgg tgccccaggt tacttgcgga gcgatctttt
     1501 cggcgtggag ttcgacggtt gtgtcgaatt tggcgccgtc atcggatttg aggagcttcc
     1561 agcggtcggc agcttcctga aattccttgc cgcgcgggac gaatggccgg ccttcgaggt
     1621 aggcaaaagt cgtttcgtcg ggagcgacca tgccggcacg ggcgccgcct tcaatggtca
     1681 tgttgcagac ggtcatgcgg ccgtccatcg agagatcgcg gattgcttcg cccgcgtatt
     1741 cggcgacgtg gccagtgccg ccgccaattc cgatgcggcc gatgatggcg aggatgatgt
     1801 ccttggcggt gacgcctttg gggcgcttgc cgtgcaccaa aatgtgcagc gttttcgatt
     1861 ttccttgcac caggcattgg gtggcgagaa cgtgttcgac ttcgctggtg ccgatgccga
     1921 aggcgagagt gccgaaggcg ccatgcgtgg aagtgtggct atcgccgcag acgatagtct
     1981 ggccgggctg cgtgatgccg agctccgggc cgatgatgtg gacgatgccc tgatttttgc
     2041 tgttcatgtc gaacaggcgg acgccggttt cctggcaatt tttggcgagc gcttcaaact
     2101 gcgctttggc gtccatatca atgatgggca gtgtgcggtc ggtggtgggc acggagtggt
     2161 ccatcacggc aaaggtcagg tcgggccggc gcaccttgcg tcgttcggcg cgcaggccgg
     2221 agaatgcctg cggggaagtg ccttcgtgga tcaggtggcg gtcaatatag aggagcgagg
     2281 gtttgccctg ctcttcgtag acgacgtgat tgttccagat cttttcgtag agagtcttgg
     2341 cggccatgta agttccctac gaagtttaac accgcaggaa aagcagggca aaaggcgcgc
     2401 agcgcaccgg taccggcaca attcgcgatg tgccgtttgg cttgcgggct taccggagag
     2461 caacggttcg cgtctcaatg tggttaggag tgttccatga agcgtcgcgc tgcagggatg
     2521 ctgttgtggt tggcagttgt gttcgcgggc tgccgcgtct ctcggcagtc aaccatcacg
     2581 cacggcgaac tagttcggcg gacgcaggaa attatggacg cgaccgcgcc ggggattccc
     2641 gacccgtggc agaaatactt cgcagaagac gcgatgtatt tcgacgaaaa agggcgggcg
     2701 atggacaagg ctgcactttt gaaggatgtg tcgccgctgc cgaaagggtt ttcaggcacc
     2761 atcaaggtgc tgaacgccag gagcaacatt ctgcgcgaca ccgcggtctt gagctacgat
     2821 ctggacgaaa ccgaaattgt ctacggccag gaattgaagg cccgttacca cggcacggac
     2881 acttggatgg agcgcaacgg ccagtggcag attatcgccg ggcagatgct gcggtattac
     2941 gaggatcctg cgccgggcgc cagcaactcc gctgaccata aaagctacgc aggcacttat
     3001 gaattggcgc cgggaattac gcgaaccgta tccactcgag gacaagactt gatggtgcag
     3061 cgatccggac gcgccgcgga aacgctgatt cccgaagcac aaggaatctt ttttcgaaag
     3121 ggagttgaag gtcgtatttt gtttcgtcgc gacgcggccg ggaaggtgga cgcgctgatc
     3181 gaccggcgga acaacgaaga tattgtgtgg aaacgcatca gctaaacgac acgcgagcaa
     3241 gccgtcgcaa gagagattag actagaaccg tggacaccat ttcttatacc tggtacgaga
     3301 gtccagttgg tcccttgctt ctggccgggt ctcacgccgc attgcgctat gtgcgctttt
     3361 ctgcgggaaa acatgccgtg gcgatcgaac cgcggtggac agaaaatcgc aacatgttta
     3421 gcgaagccat tcggcaatta tcagaatatt ttgccggaga gcgaaaaacg ttcacgctgg
     3481 aactgggtcc gcccggcacg gaatttcagc gatcggt
//
LOCUS       DASTPM010000639         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_974331, whole genome shotgun sequence.
ACCESSION   DASTPM010000639 DASTPM010000000
VERSION     DASTPM010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974331"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1618)
                     /locus_tag="VFR42_11270"
     CDS             complement(<1..1618)
                     /locus_tag="VFR42_11270"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU0369786.1"
                     /translation="MPKIKRRSFIEMAAAIGATAAWGNPFGSESRTPWHERRDLFVEG
                     VASGDPDSTSVLLWTRYSAKGANAPEELRVEVAEDTAFKRVVAATSTHASPAADWTCR
                     VLTGGLKPAHLYWYRFSDKDGNGSRIGRTRTAPGLDDPRPVSFAFVSCQNVTQGAQNA
                     YRRMIFEDERAPEAEQIEFVLHLGDFIYEITWYPEDRPQGMYDRKIREIYRYPEGEKI
                     RDFHIPTTLEGYRTVYKGYLHDPDLQDARARWPFVNMWDNHEFSWLGWQSFQKFQSKT
                     RPAQTRKVAANQAFFEYQPARMARPNSHSLEEFQPAVVTDAPVTKFDENGLGQEPNNL
                     AAIGSLKGYRAIRWGRNAELIVTDQRTYRSEEPTDMPEAKGLVSDDFPELIPEEPIKI
                     LDAARACNGGKPPATIRSGDGSIELPNIWRERPPQTILGAEQKVWFLDRLKSSKATWK
                     IWGNTTATLDMRADPQNLPAGTGKPWPWTGYASFAGSGDHSTAFFERGEIYDFVREHG
                     ITGFATVAGDRHSFWAGLAAKSLPPKLFQPV"
BASE COUNT          432 a          678 c          656 g          508 t
ORIGIN      
        1 caaccggctg aaacaatttg ggtggaagcg atttggcggc gagtccggcc cagaagctgt
       61 gccggtcgcc cgcgactgtg gcgaagcccg tgatgccgtg ctcccgcacg aagtcgtaaa
      121 tctcgcctcg ctcgaaaaac gccgtgctgt ggtcgccact gccggcgaag cttgcgtagc
      181 cggtccacgg ccagggcttg cccgtgccag caggaagatt ttgggggtcg gcgcgcatgt
      241 cgagggtcgc cgtagtgtta ccccatattt tccaggtagc cttggagctt tttaggcgat
      301 ccaggaacca gaccttctgt tcggccccca ggattgtctg cggcggacgt tctctccaga
      361 tgttgggaag ttcgatgctg ccgtctccgg atcggatcgt cgcgggcggc ttgccgccgt
      421 tgcaggcacg cgccgcatca agaattttga tcggttcttc gggaatcaac tcgggaaaat
      481 cgtcgctgac gagtcccttc gcttcgggca tgtcggttgg ctcttccgag cgataggtgc
      541 gctgatctgt gacgataagc tccgcgttgc gaccccagcg aatcgcgcgg taacccttca
      601 ggcttccaat ggcggcgagg ttgttcggct cctggcccag accgttttcg tcgaattttg
      661 tgacgggagc atccgtcacc accgcaggct ggaattcctc caaggaatga ctgttcggcc
      721 ttgccatccg ggccggctgg tattcaaaga acgcctgatt cgcagccact ttccgcgtct
      781 gcgcggggcg cgtcttgctt tggaacttct ggaaggactg ccagccgagc cagctgaatt
      841 catggttgtc ccacatattg acgaaaggcc agcgggcccg cgcgtcttgc aggtcgggat
      901 cgtgcaggta tcctttatat acggtgcggt agccttctag ggtggtgggt atgtgaaagt
      961 cccgtatttt ttctccttct ggataccgat agatctcgcg gattttgcgg tcgtacattc
     1021 cctgagggcg atcttcagga taccaggtga tttcatagat gaagtcgccc agatgcagga
     1081 cgaattcgat ctgttccgct tccggcgcgc gttcgtcttc aaaaatcatt ctgcggtaag
     1141 cgttttgcgc gccctgcgtt acgttctgac aactcacaaa cgcgaaggag actggtcgag
     1201 ggtcatccag gcctggcgcg gtccttgtcc gcccaatacg gctaccgttg ccgtccttat
     1261 ctgagaagcg ataccagtag agatgcgccg gtttcaggcc ccccgtcaag acgcgacagg
     1321 tccagtccgc ggcgggagac gcgtgcgtgc tcgtcgcggc aacgacacgc ttgaacgccg
     1381 tgtcttcggc cacttctact cgcagctcct ctggagcgtt cgcccctttc gctgaatacc
     1441 gggtccacaa aagaacactc gtgctgtccg ggtctcctga agcgactcct tccacaaaca
     1501 agtcgcgccg ttcgtgccaa ggagttcggg attcggatcc aaacggattg ccccacgcgg
     1561 cggtggcgcc gatcgccgca gccatctcga tgaacgagcg ccttttgatc ttcggcattg
     1621 tgtccttccc caaaaagaaa tggatccgac ctcgctagct cgccgctgcg gtctccgggc
     1681 gtatggcgat cggacgatca atctgtgtct tgagaacgat cgtcgcctcc gtctgttcga
     1741 cggctggaat cgcgcgaagg cgttcggatc ctacccgcaa atggctagtg attctcaaga
     1801 atcagcaatg ttcccttttt tcagagaagg cgcaccgcgt cggagtccga tgcttccaaa
     1861 tcgaagtgca atgaaattcc acatctccgc cgtgcgtatg caatatgtgg gatcaccata
     1921 tatgcctcta cctgccgagc acgagtggaa gaaaggcagt gccgagcttc tggttctatc
     1981 ccagctcgaa gaccagcccc gccacggcta cgaaatctcc cgtctcatcg accttcgtta
     2041 gggtggctcc atccactttc acgtagcctc gctgtaccca ttgctctgcc tcgaaaaagc
     2101 gtaactggat ccaaagccgc tggctcgaga aagcgaatca acgccgccgc cgctactacc
     2161 gcctgaccgc gcaaggcgag cgcgttttgg cggacaagcg ccgccgatgg caagaatttg
     2221 caggcgcagt cgcccgaatc atgggagtgc aacatgcctg actggaaatc cgaa
//
LOCUS       DASTPM010000640         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_771702, whole genome shotgun sequence.
ACCESSION   DASTPM010000640 DASTPM010000000
VERSION     DASTPM010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771702"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..852
                     /locus_tag="VFR42_11275"
     CDS             130..852
                     /locus_tag="VFR42_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369787.1"
                     /translation="MKCSTVRYRLAGYLDDVVETPARSSERSEMRVHLDSCAVCREEL
                     QRYRKLAVLLSRAPRALPPSSLAVRIKVAAAQVQHPLDPRTRWQRFRDRAEILLDNVF
                     RPLTVPATGGFISAMLIFVVVLQVMAPGLTVQANPNDVPLNLMQPAELVALSDSPGSW
                     APEKHDSELALPHGLLVDVTVDQDGRMVDYQILAGPRTPDMQHQLNEMLLFSRFTPMK
                     SFGRKISGGHVILSFSAVRVRG"
     gene            1008..1802
                     /locus_tag="VFR42_11280"
     CDS             1008..1802
                     /locus_tag="VFR42_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU0369788.1"
                     /translation="MSGTLPHRIPRLLAAFALAALLGLWLVPRASSTPAGRPSTSSEQ
                     MLYDAVNRERASLGLHQLQWDTALANAARLHTSLLAEHDALSHRFNGEADLQTRLRMA
                     GANFRLVAENVAQAPDVESLHVAWMNSPPHRANILDPQVNSIGIAIVRRGNELYATQD
                     FSASVVQMNKEEQEQQVARLLQASGLGVIANAEEARKSCDQNRAFYTTQPMTISRFET
                     SDLSRLPNELARMTSSGRFRRAAVGACEVPTVTPFARFRLTVILYQ"
     gene            2053..>2818
                     /locus_tag="VFR42_11285"
     CDS             2053..>2818
                     /locus_tag="VFR42_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369789.1"
                     /translation="MKIYNVIGYSIILCYMLACMYFAPARLGPWTGMLIGGVYFVFCW
                     FLGGLYLADVLHLGIAHRSLDYKEWFIKAVTVVNNIFGLYVDPIAWVNRHRLHHKHSD
                     HPGDPNKLSSDGFWRTLYLCLMPYRCQENLAGDKILKSWTFRVVANPFFAIFAQGFSF
                     YLLWKLVGSLKFTLVMWIGMRIFAAWVNMIQNYWTHTREFGYRRYHDEEDNAMNIGEW
                     LPVTATFSACLQNNHHHYEGLLRLSHDKSEYDFGFLT"
BASE COUNT          557 a          851 c          739 g          671 t
ORIGIN      
        1 ggcacacggt ttctcggcag gtacgctcgc tccagcgctg gtaaaggagc ggatgaattt
       61 cgcgccatcg ttaaatcttt tcgacgaatt ttccggcgcg gattcggtgc aaggcagaga
      121 gggcaagcca tgaagtgctc cacggtccgg tacaggctgg cggggtacct cgacgatgtc
      181 gtcgaaaccc cggcgcgctc cagcgaacgc agtgaaatgc gcgtccatct cgactcttgc
      241 gcggtttgcc gcgaagagct gcagcgctac cgcaaacttg ccgtattgct ttcccgcgca
      301 cctcgcgctt tgcctccgtc cagtctcgct gttcgcatca aagtcgccgc ggcccaggta
      361 caacaccctc tcgatccgcg tacacgctgg cagcgtttcc gtgatcgtgc cgaaatcctc
      421 ttggacaacg tttttcgtcc gctcaccgtt cccgccactg gcggtttcat ttccgccatg
      481 ctcattttcg tggttgtgct gcaggtcatg gctcctggcc tgaccgtgca ggccaatccc
      541 aacgacgttc cgctgaacct catgcagccc gccgaactcg tcgcgctctc tgattcgccc
      601 ggcagctggg ctccggaaaa acacgattca gagttggcgc ttccgcacgg tttgctggtc
      661 gacgtcaccg tggatcaaga cggccgcatg gtcgattatc agattcttgc cggcccgcgc
      721 accccggaca tgcagcacca actcaacgaa atgctgctct tttcccgctt cactccgatg
      781 aagagctttg gccgcaaaat ttccggcggc cacgtcatct tgagcttcag cgcggtccgc
      841 gtccgcggct agctctgtcc gcgccgctta cagtattcac tcgattccca ttgcccaacg
      901 gaaattgaaa gaatcctggc ctgtacttcg gaacagtgct ttcgtccctg cgtacctgtt
      961 cccgcgttcc attttcatga gcaatccagc ccgctctaat tacctccatg agcggaacgt
     1021 tgcctcaccg cattccccgc ttgctcgcgg cattcgccct ggccgcgctg ctggggctgt
     1081 ggcttgtccc gcgcgcctcc agcacgccgg ccggccgtcc ttccacttcc tcggaacaga
     1141 tgctctacga cgccgtcaac cgcgaacgcg cttcgctcgg tttgcatcaa ctacaatggg
     1201 acaccgcgct agccaacgcc gcccgcctgc acacttctct gcttgccgaa cacgacgccc
     1261 tatcccatcg ctttaacggc gaagccgacc tccagacgcg cctgcgtatg gctggcgcca
     1321 acttccgcct ggtcgccgaa aacgtagccc aggcccccga cgtggagtcg ctccacgtcg
     1381 cctggatgaa ctctccgccg catcgcgcca acatcctgga tccgcaggtg aactccatcg
     1441 gcatcgcgat tgtgcggcgt ggcaacgagc tttacgcgac gcaagatttt tccgcgtcgg
     1501 ttgtgcagat gaacaaggaa gagcaggaac agcaagtcgc tcgactgttg caggccagcg
     1561 gccttggcgt catcgcgaac gcggaggaag cacgcaagag ctgcgaccag aaccgcgcct
     1621 tttacaccac ccaacctatg accatttccc gctttgagac ttcggacttg agccgcctgc
     1681 ccaatgaact ggcgcgcatg acctccagtg gccgcttccg ccgcgcggcg gtgggcgcct
     1741 gcgaagtccc cacggtcact ccgttcgcgc gcttccgcct caccgtcatc ctttatcagt
     1801 aattccgaaa ttaaatttta acttagaaag cacgaacgta attgttcgcg cctactaagc
     1861 cgcagaaatt ttacgcgtct gcaaagctta ttcaggaaac aacgcggagc gaacagggca
     1921 gtgctagaga cgctggaatt ctgatggtgg ggatacgatt tcctgcgcat tcaagacgca
     1981 gcgacatctt cttcgacttg ttatttgaaa ggagctctca tttctgtttg aattgccccg
     2041 cggggaatta tcatgaaaat atacaacgtc ataggttatt cgattatttt gtgctacatg
     2101 ctggcttgca tgtatttcgc cccagctcgc ttgggaccgt ggactggaat gttgatcggc
     2161 ggagtttatt ttgtcttttg ttggtttctt ggcgggttgt atttggccga cgttcttcac
     2221 ctgggaatcg ctcatcgttc actcgactat aaggagtggt tcataaaagc cgtcacggtc
     2281 gtcaataaca tcttcgggct ctatgtagac ccgatcgcct gggtgaatcg ccatcggttg
     2341 catcataagc attccgacca tcctggcgat cccaataaac tttcaagcga cggcttttgg
     2401 cgaacgctgt atctctgcct gatgccgtat cgatgccagg agaatttggc cggcgacaag
     2461 attttgaagt cctggacttt tcgagttgtt gcgaacccgt ttttcgcaat attcgctcaa
     2521 ggcttcagtt tttatctctt gtggaagttg gttggaagtc tgaagttcac gctggtgatg
     2581 tggattggaa tgcgaatttt cgcggcgtgg gtgaatatga ttcaaaacta ctggacccac
     2641 actcgagaat ttggctaccg gaggtatcac gacgaagaag ataacgccat gaacatcggc
     2701 gagtggctac cggtgacggc gacctttagc gcgtgcttgc aaaataatca tcaccactat
     2761 gaggggctct tgcggctgag ccacgacaag tctgaatacg actttggatt cttgacgg
//
LOCUS       DASTPM010000641         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1089676, whole genome shotgun sequence.
ACCESSION   DASTPM010000641 DASTPM010000000
VERSION     DASTPM010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089676"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="VFR42_11290"
     CDS             complement(<1..1639)
                     /locus_tag="VFR42_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEU0369790.1"
                     /translation="MKIAHARFLAASVLFLALVLLPLPSAAQWNPLNPVQSSQTDTVG
                     LTIFLERGALRFQVCTDSMIRVLYSPQHEFPRVTEYVVIKSAWPKTPFDVNEAADNIT
                     LTTAKLKVVVVKKDSSIIFYDGAGRKLAQENDRTLTPVEVNGEKTNHLERFTNMWDTQ
                     EAFYGLGQHQGGVFNWRGESVDIVQDNTNISIPLLLSSNGYGIFWNNPSRSRFNNRFV
                     HAFYISSEVAEQMDYYFLYGPDFDRIIGAYRELTGAAPLFGKWAYGYWQCKNRYDSQA
                     EILGVAKRYRSEHIPVDNIVQDWFWWNTMGEPVWNKNYPDPKAMVAELHQENLHLMVS
                     VWPFFRPGSPVYDDMAAKGFFIDKTRVTGFHPAGMALYDAFNPEARKYYWNLMDDALF
                     KIGVDAWWLDTTEPETEGREESVLLHNKVRVDLAAESGARYANLFPLMTTAAVYQGQR
                     AASDQKRAFILSRSAFAGSQRNAAAVWSGDVNPNWETLRRQIPAGLNYSLSGLPYWTT
                     DIGGFVVADPQSPAYRELYARWFEFGTFCPIFRAHGTR"
     gene            complement(1704..>2851)
                     /gene="galE"
                     /locus_tag="VFR42_11295"
     CDS             complement(1704..>2851)
                     /gene="galE"
                     /locus_tag="VFR42_11295"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU0369791.1"
                     /translation="THCDEGRLRFVHVPEITLRANAYYNDAALSFLRTRLLFSERPAK
                     FMAERILVAGGAGYIGSVVAAQLLARGYEPVVYDDLSHGHRAALPQSVRLIVGDIGDR
                     AALDRTFSEVQPQAVMHFAAFIEAGESMQFPEKYFRNNTASALTLLEAVLAHKVSRFV
                     FSSTAALYGTPEKTPIEETAPLHPTNAYGESKLLVEQMLEWLHRIHGLRYASLRYFNV
                     AGAADEQGEDHHPESHLIPLAFQVALGTRKEFAIFGTDYPTPDGTCIRDYIHVSDLAT
                     AHLLVLEALKEKDRLIYNLGNGQGFSVHEVIETVRRVTGHPIPAKESPRRPGDPAVLV
                     ASSEKIKKELGWQPKFADLENIVRSAWEWRKAHPRGYAPNRSATNLS"
BASE COUNT          614 a          738 c          898 g          601 t
ORIGIN      
        1 tgcgcgtgcc atgcgcgcgg aaaatcggac agaacgttcc aaattcaaac cagcgcgcgt
       61 agagttcgcg atatgcagga ctctgcggat cagctacgac aaagccgccg atatcggtcg
      121 tccaataagg aaggcccgag agcgaatagt tgagcccagc gggaatctgg cggcgtaagg
      181 tttcccagtt gggattcacg tcgccggacc agactgcggc ggcgttgcgc tgcgatcctg
      241 cgaatgccga gcgcgacaaa ataaaagcgc gcttttggtc gctggcagct cgctggccct
      301 gatagacggc agccgtagtc atgagcggaa agagattggc gtagcgcgcg ccgctctctg
      361 cggctagatc gacgcggact ttgttgtgaa gcaagacaga ttcttcgcgg ccttcggttt
      421 cgggttcggt ggtgtcgagc caccaggcat ccactccgat tttgaaaagc gcgtcgtcca
      481 tcaggttcca gtaatatttg cgtgcttcgg gattgaacgc gtcgtaaagc gccatgccgg
      541 cgggatgaaa accggtcacc cgggttttgt cgatgaagaa cccttttgcg gccatgtcgt
      601 cataaaccgg tgaaccggga cggaaaaagg gccacacgga aaccatcaga tggaggtttt
      661 cctggtgaag ttcggccacc atcgctttcg gatcgggata attcttgttc cagaccggct
      721 cgcccatggt gttccaccag aaccagtcct gcacgatgtt gtcgaccgga atgtgttcgc
      781 tgcgatagcg tttggcgacg ccgagaattt ccgcctgcga atcgtagcga ttcttgcact
      841 gccagtagcc gtaagcccat ttgccgaaga gaggcgcggc tccggtcagt tcgcgatacg
      901 cgccgataat gcggtcgaag tccgggccgt agaggaagta gtagtccatc tgttcggcga
      961 cttcggagct gatgtagaac gcgtgcacga agcggttgtt gaagcggctg cgcgacggat
     1021 tgttccagaa aattccgtag ccgttgctgg agagcagcag cggaatggaa atgttggtgt
     1081 tgtcctgcac gatgtcgacg gactcgccgc gccagttgaa cacgccgccc tgatgctggc
     1141 ccaggccgta aaacgcttcc tgggtgtccc acatattggt gaagcgctcg agatgattgg
     1201 ttttttcgcc gttcacttcg accggcgtca aagtgcgatc gttttcctgc gcaagcttcc
     1261 gtccagcacc gtcgtagaaa atgatcgagc tgtctttttt aacaacgaca actttcagct
     1321 tggcagtggt gagcgtgatg ttgtcggccg cttcgtttac atcgaatggc gtcttaggcc
     1381 aggcggattt gatgacgaca tattcggtga cgcggggaaa ttcgtgctgc ggggaataca
     1441 aaacgcggat catcgagtcg gtgcaaacct gaaaacggag cgcgccgcgt tcgaggaaaa
     1501 tcgtcaagcc gaccgtgtcc gtttgtgagg attgaaccgg attcagaggg ttccactggg
     1561 cggcggaggg gagaggaaga agaaccagag cgaggaagag gaccgaagca gcaagaaagc
     1621 gggcgtgtgc gattttcaag aaaatgccca tagtaagcgt ttacgatgac cgcacgggac
     1681 tttcgcacct ccgtttccgg atgtcaagaa aggttggtgg cggaccggtt gggcgcgtag
     1741 ccccgagggt gtgccttgcg ccattcccag gcgctgcgga cgatgttttc taggtctgcg
     1801 aacttcggct gccagccgag ttccttcttg attttttccg agctggcgac gaggaccgcg
     1861 ggatcacccg ggcgacgcgg cgattctttc gcgggaatgg gatgcccggt tacgcggcgg
     1921 acggtttcaa tcacttcgtg cacgctgaag ccttgaccgt tgccgagatt gtagatgagg
     1981 cggtcttttt ccttgagcgc ttccagcacc agcaaatgcg cggtggcgag atcgctcacg
     2041 tggatgtagt cgcgaatgca ggtgccatcg ggcgtcgggt agtcggtgcc gaagatggcg
     2101 aactctttgc gcgtgccgag cgcgacttga aacgccaacg ggatcaagtg cgattcggga
     2161 tgatgatctt cgccttgttc gtccgcggca cccgcgacat taaaataacg caacgacgca
     2221 tagcgcaacc cgtggatgcg gtgcagccat tcgagcatct gctcgaccag cagtttcgat
     2281 tcgccataag cattggtggg atggagcgga gcagtttctt cgatgggagt tttttccggc
     2341 gtgccataaa gggccgccgt ggaagaaaag acaaaacggg aaaccttgtg tgctagaacc
     2401 gcttccagca gagtcagcgc gctggcggtg ttattgcgaa aatacttttc ggggaactgc
     2461 atggactcgc cggcttcgat gaaagcggca aagtgcatga cggcttgcgg ctgaacttcg
     2521 gaaaaagtgc ggtcgagcgc cgcgcggtcg ccgatatcgc cgacaatcaa gcggacactt
     2581 tgaggaagcg ctgcgcggtg gccatgcgag agatcgtcat acacgaccgg ttcgtaaccg
     2641 cgcgccagca attgcgcggc gactacgctg ccgatgtaac ctgcaccgcc agccaccagg
     2701 atacgttccg ccatgaattt cgccggcctc tctgaaaaaa gcaatcgagt acgaaggaat
     2761 gaaagcgcgg catcattgta atatgcgttc gcgcggagag tgatttcggg gacgtgaacg
     2821 aagcgcaggc gtccttcgtc acaatgagtg c
//
LOCUS       DASTPM010000642         4392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1263020, whole genome shotgun sequence.
ACCESSION   DASTPM010000642 DASTPM010000000
VERSION     DASTPM010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263020"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1138
                     /locus_tag="VFR42_11300"
     CDS             212..1138
                     /locus_tag="VFR42_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU0369792.1"
                     /translation="MISAYHRGVHRFAVFVLLWTVLLFVAGALVTSNAAALSVPDWPK
                     SFGTWFPSLRQLAKGGALFEHSHRVIAGVLGVLLLAEAVVIWLKDERKWLRWFAAIAV
                     GGVVVQSILGGEVVRRLLQYWLPVLHACFAQIMFGAILCMAVFTSKWWIEEHKQLEDR
                     GGISIHSIALLNAMVMFVQVFLGAGFRHQYVAIWPHIVGAGVVLAVMIWTAAVLRRRF
                     DDSPELSFGLALLHSMVGLQILLGIAAYWSRLITADAPQPMPVMVWLTVIHTVYGALV
                     FAASILVVLLCYRLVPRSGTVAVPSRPRTAAE"
     gene            1135..2028
                     /gene="cyoE"
                     /locus_tag="VFR42_11305"
     CDS             1135..2028
                     /gene="cyoE"
                     /locus_tag="VFR42_11305"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU0369793.1"
                     /translation="MSTFRTTELTLGGRANAYVTLTKPDVSFLVLMTTAAGYYMGARG
                     PVDWLNLVHTVFGTMLIAGGTAALNHYIERDSDRHMRRTASRPLPSGVLQPAEALWFG
                     VELSVAGAVDLWIMAGWLASLLGAITCLSYLLAYTPLKKRTVWATFVGAFPGAVPPMI
                     GWAAATRHLDGGAWLLFLILFFWQFPHFNAIAWMYREDYARAGIRMLSVGDVDGSRTF
                     RQILISAAMLVVISLLPSVLRMTGMLYFFGALVVSTALVQVCVWAAYSKSNARAKWLM
                     HATVLHIPILLALMAYDKIAR"
     gene            2062..2487
                     /locus_tag="VFR42_11310"
     CDS             2062..2487
                     /locus_tag="VFR42_11310"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEU0369794.1"
                     /translation="MIFPHAALNATLNATSAILLAIGYAAIRAKKIEAHKRFMISAFV
                     VSCAFLVSYLVYHYRVGHVVFRGQGIIRPIYFVLLTSHTILAVVIVPMILVTLRRAWL
                     ERFDKHRLIARWTLPMWFYVCVTGVIVYLMVYQFCAPCS"
     gene            complement(2709..3728)
                     /locus_tag="VFR42_11315"
     CDS             complement(2709..3728)
                     /locus_tag="VFR42_11315"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HEU0369795.1"
                     /translation="MKHHVTLLPGEGIGPEVSRAVRRILEAAGVQIDWEEIDARAPDT
                     PASAKANGGSVLNQTAVDSVRRNGVALKGPMATAVAGGAPSVNVALRKTLDLYANLRP
                     VRNLPGLKTRFENVDVVIVRENTEDLYSGLEHEVVPGVVESLKIITEKASTRIARFAF
                     EYAKREGRKKIHAIHKANIMKLSDGLFLRSIRTVAAEYPGIEYKEMIVDNACMQIVLD
                     PQQFDILLLPNLYGDVMSDLAAGLVGGLGVVPSGNIGDHGAMFEAVHGTAPDIAGKGF
                     ANPTALLLSSILMLDHLGERSAARRIEAALETVYRGAKHTTKDVGGSATTDQFTDAVI
                     AALPH"
     gene            3912..4238
                     /gene="erpA"
                     /locus_tag="VFR42_11320"
                     /pseudo
     CDS             3912..4238
                     /gene="erpA"
                     /locus_tag="VFR42_11320"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
BASE COUNT          825 a         1065 c         1422 g         1080 t
ORIGIN      
        1 atcgacgggc gcattgatgc gaagaaatac gggatcgaac ggtttcgcat gatgccggcg
       61 gtcggcgatt cgcctgcgtt catcggagcg ctgaaagatc tagtgctgca aaagctggaa
      121 atttcagacg aaaaatccga gctgcgcgcc gctggctgag ttcgcaggct attcagtgtt
      181 ggagtataat ttccctgcgg aaatttcaat aatgatctcg gcttatcatc gtggagtaca
      241 tcgtttcgcg gtatttgtgc tgctttggac cgtcctgttg tttgtagccg gagcgttggt
      301 aacttccaac gcggcagcgc tttccgtccc tgattggccg aaatcgttcg ggacgtggtt
      361 tccttcgctg cggcaactag cgaagggtgg cgcgttgttt gaacattccc atcgcgtgat
      421 cgctggagtt ttgggtgtgc tgcttctagc cgaagcggtg gtgatctggc tgaaagatga
      481 gcgcaagtgg ctgcggtggt ttgctgcgat cgcggtgggc ggcgtggtcg tgcaatcgat
      541 tcttggcggg gaagtagtgc gcaggttgct gcaatattgg ttgccggtgt tacatgcgtg
      601 ttttgcgcaa ataatgtttg gtgcgatatt gtgcatggcg gtgttcacca gcaagtggtg
      661 gattgaagaa cacaagcagc tggaagatcg cggcgggatt tcgattcatt cgattgcact
      721 tctgaacgct atggtgatgt tcgtccaggt atttctcggg gccggttttc gccatcaata
      781 cgtcgcgatc tggccgcaca tcgtgggtgc gggagtcgtc ctcgcagtga tgatctggac
      841 ggcagcggtg ctgcgtagaa ggtttgacga ttcgccggag ctgtcatttg ggctggcgct
      901 gttgcactcc atggtcgggt tgcagattct gctgggtatc gcggcatact ggtcgcggtt
      961 gatcacggcg gatgcgccgc agcctatgcc cgtgatggta tggctgacgg tgattcacac
     1021 ggtgtatgga gcactggtct ttgccgcttc gattctggta gttttgctgt gctatcgtct
     1081 ggttccgcgg tcgggaacgg tggcggttcc ttcgcggccg cgaacggcgg ctgaatgagc
     1141 acgtttcgca caaccgaact gactcttggg ggccgcgcca acgcgtatgt gacgctaact
     1201 aaacccgacg tgtcgttcct ggtgctgatg accacagcgg cgggctacta catgggcgcg
     1261 cgcgggccgg tggattggct gaacctagtg cacacggttt tcggcaccat gctgattgcc
     1321 ggcggcaccg cagcgttgaa tcactacatt gaacgcgatt cggatcgaca catgcggcgc
     1381 acagcttcgc ggccgttgcc gagcggtgtg ctgcagcccg cggaagcact ttggtttggt
     1441 gtagagctca gcgtggcagg agcggtagat ctttggatta tggccggttg gctggcttcg
     1501 ctgttgggcg cgatcacctg cctgagttat ttgctggcgt acacaccgct gaagaagcgt
     1561 accgtgtggg cgacatttgt cggtgcgttc cccggcgcgg tgccgccgat gatcggctgg
     1621 gccgcggcga cacggcatct ggatggcggc gcgtggctgc tgttcctgat tctgttcttc
     1681 tggcaattcc cgcatttcaa tgcgattgca tggatgtacc gcgaagatta cgcgcgcgcg
     1741 ggcattcgca tgttgtcggt cggcgacgtg gatggttcac ggactttccg gcagattttg
     1801 atctctgcgg cgatgctggt ggtgatcagc ctgctgccat cggtgctgcg catgaccggc
     1861 atgctgtact ttttcggtgc gctggtggtg agcacggcgc tggtacaagt gtgcgtttgg
     1921 gcggcgtaca gcaagagcaa tgcccgcgcg aagtggctga tgcacgccac ggtgctgcac
     1981 attccaatac tgctggcgct gatggcctac gacaagatcg cccgataacc aggttgtatt
     2041 gcgaactcag cttccatcta aatgatattt cctcacgccg ctctcaacgc caccctgaat
     2101 gcaaccagcg caattttgct tgcaatcggg tatgccgcga tccgcgccaa aaaaatcgaa
     2161 gcgcacaagc ggttcatgat ttcggcgttt gtagtgtcgt gcgcgtttct ggtttcctac
     2221 ctggtgtatc actaccgagt cggacatgtc gtgtttcggg ggcagggaat catccggccg
     2281 atttattttg tgctgctgac ttcgcacacg attctggccg ttgtgattgt gccgatgatt
     2341 ctggttacgc tacgacgcgc ctggctggag cgattcgata agcatcgctt gattgcgcgt
     2401 tggacgctgc cgatgtggtt ctacgtgtgt gtcaccgggg tgatcgtgta tttgatggtc
     2461 tatcagtttt gcgcaccgtg tagctagcgc gacgctactt ctcatagtcg tattttggca
     2521 gcgcaggcgg gggcctatat aagcctcaca ccttgggcat gacctggcaa agagccagaa
     2581 ttgtgaagca aagcagggaa ctactcagat cgcgccaaca gttaaaatgg gcatttcctt
     2641 acgaacaaac tactagacgc ggcctgccag cagcacataa gggctaatga cagtaatacc
     2701 atgagcagtt aatgcgggag ggccgcgatt acggcgtcgg tgaattggtc tgtcgtcgcg
     2761 gagccgccta catctttggt ggtgtgcttg gcgccacggt agacagtttc gagggcggct
     2821 tcgatgcggc gcgcggcgga acgttcgccg aggtggtcga gcatcagaat gctgctcagt
     2881 agaagggccg tgggattcgc gaagccttta cctgcgatgt cgggcgcggt gccgtggacg
     2941 gcttcaaaca tggcgccgtg atcgccgatg ttgccgctgg ggacgacacc taagccgccg
     3001 accagtcctg ccgccagatc gctcattaca tcgccataca aattgggcag caacagaata
     3061 tcgaactgct gcgggtcgag gacaatctgc atgcaggcgt tatccacgat catttctttg
     3121 tattcgatgc cgggatattc ggcggctacg gtgcggatgg agcgcaggaa gaggccgtcg
     3181 gaaagcttca tgatgttggc tttgtggatg gcgtgaattt tcttgcggcc ttcgcgcttg
     3241 gcgtactcga aggcgaagcg cgcaatgcgc gtagaagctt tttcggtgat gattttgagg
     3301 ctttcgacga cgccgggaac gacttcgtgt tcgaggccgg agtagaggtc ttcggtattt
     3361 tcgcggacga taaccacgtc gacattttca aaacgcgttt tgaggccggg aagattgcgc
     3421 accgggcgaa gattggcgta aaggtcgagc gtcttgcgga gcgcgacgtt gacgctgggc
     3481 gcgcctccgg ctacggcagt ggccatgggg cctttcagtg cgacaccatt gcggcgcacg
     3541 gaatcgaccg cggtttgatt taagaccgag ccgccgttgg ctttggccga ggctggggtg
     3601 tcgggggcac gggcgtcgat ttcttcccag tcgatctgga cgccagcggc ttcgagaatg
     3661 cggcggacgg cgcgggagac ttcagggcca atgccttcgc cagggagcag cgtaacgtga
     3721 tgtttcatag atgcatctta ttgtatgcaa aggagttcgg gatgcgaaag gtccctgcgg
     3781 aagcgtttcc tcggcggaac cttgcagatt agaatgttgg ctccagttgc gatgtgcact
     3841 cagatttcga ttctgttaaa atagggtggt aggattttgc cgcggaatcc gccgcggcgg
     3901 aggctaaaga agtcatgatc agtcttactc cggttgccag tagcaaggtg aaggaaatca
     3961 tgagcatgca gaacccggtg ccgagcgcat tgcgcgtggc cgtggtggga ggcggctgct
     4021 cgggctttca gtaccacatg gcttttgaaa accagaccaa tgacagcgat gaagtggtgg
     4081 atttcgacgg tctgaaagtg gcagtggacc agatgagttc gatgtatctg gaaggcgtgg
     4141 agattgacta catcgaaacg ctggaaggcg cggggttcaa gttcaacaac ccgaacgtga
     4201 aaagcacttg cggctgcggc agttcgttta gcgtgtaaag ggcatttcaa gagtttaagg
     4261 ttgaaaagtt caaggctcct gagagggaat tagttccgct caggagcctt ttttcttttg
     4321 tatgtcattc atgtctggct gtttgcggcc cggtcgggtt gacattgctg cacagattga
     4381 agtttgtgct ac
//
LOCUS       DASTPM010000643         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_23212, whole genome shotgun sequence.
ACCESSION   DASTPM010000643 DASTPM010000000
VERSION     DASTPM010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23212"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2602
                     /gene="alaS"
                     /locus_tag="VFR42_11325"
     CDS             <1..2602
                     /gene="alaS"
                     /locus_tag="VFR42_11325"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU0369796.1"
                     /translation="LENVGFTKRHHTFFEMLGNFSFGDYFKKDAIVYAWELVTSPEWY
                     GIAKDKLYVTVFGGAEVSPGSKLGVDEEAKTFWLGQNLPAERIVPIPGLKDNFWAMGD
                     TGPCGPCSEIHYDMGPEASEQGHADCKFPCDCGRFVEIWNLVFMQFNRDAGGTLTPLP
                     KPCVDTGMGLERVAAVLQGVISNYDTDLFVPLMKRAAELCGVDFAREEKLEEGKGGAA
                     SLRVIADHARATTFLMTDGVLPSNDGRGYVLRKIIRRAIRHGRLLGATKPFLSYTVQT
                     VRELMGRAYPELTESAAARVPEIVLSEEKRFGATLDAGLIKLTEELRPLLQLRAQYED
                     ASKQLDSTPGGPQRVELSQKRSELSRKMTYSGELAFKLFDTYGLPRDFVDDVCRDAGI
                     PVSWDEFDRAMQEQRRKARASWKGTHKEAASPAYAKIAETYKTEPGFYYGTHAKDCRI
                     EAIISKAGAVNELKAGESAEVVLDRTAIYAESGGQIFDTGALYNDSETQLLAEVSGAY
                     YPVAGLVAHRVSAKEELRVGDRVAVVADADRRARVMRNHTGTHLVHAALRNILGTHVK
                     QAGSLNAPDRLRFDFSHFAPVYAEELRDIEQQVNEEIRLNTEIETNVTTLDDALASGA
                     LAFFGDKYPEHNVRVVTIPDPHAPRGFYSKELCGGTHVKRIGDIGVLKIVSEESVAAG
                     VRRVEAITGIGALEHYQHQASVLASVAKQLNVGEDALLAQVEKLIQTVRQLEKLLTDQ
                     RRKGALNQLDDLVAKAQTIKGTKVVVSEVENVDREALRQLVDSLRQKLGSGVVVLGQA
                     EDGKVALIAGVTKDLTAKVHAGKVIQAMAKLVNGKGGGRPDLAEGGGEDTKALNSTLG
                     GVPALLEGLL"
     gene            2782..>3282
                     /locus_tag="VFR42_11330"
     CDS             2782..>3282
                     /locus_tag="VFR42_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU0369797.1"
                     /translation="MGKRSSNPVLVARAILACSGAFFAAAANAQIAKVSNSDGRMFFV
                     NADTPTALKFASRKPRAIFLPAEVSFTGGNRPAMDLNRDGAEKLVREAAERHSVDPAL
                     IRAVIETESNWNAGAISRKGAVGLMQLIPTTAQRFGVNDLYSPQQNVDAGVRYLKTLL
                     ERYNGNL"
BASE COUNT          725 a          936 c          991 g          630 t
ORIGIN      
        1 cctggaaaat gtcggcttca ccaagcgtca ccacaccttt tttgaaatgc tcggcaactt
       61 ttcctttggg gactatttca agaaggacgc gattgtgtac gcttgggagc tggtcacttc
      121 gcccgagtgg tacggcatcg ccaaggacaa gctctacgtg acggtgtttg gcggagcgga
      181 agtttcgccc ggcagtaagc ttggtgtgga tgaagaagcg aaaacctttt ggctcgggca
      241 aaacctccca gccgagcgca tcgtccctat tcccggattg aaagacaact tctgggccat
      301 gggtgacacg ggtccctgcg gcccatgcag cgaaattcat tacgacatgg gcccggaagc
      361 gtccgagcag ggtcacgccg attgcaaatt tccctgcgac tgcggccgct tcgtcgaaat
      421 ctggaatctc gttttcatgc agttcaaccg cgacgccggc ggaacgctca ctccgctccc
      481 caagccttgc gtcgataccg gcatgggact tgagcgtgtc gccgcagtcc tgcaaggcgt
      541 aatctccaac tacgacaccg acctgtttgt tccgctgatg aaacgcgctg ccgaattgtg
      601 cggcgtggac ttcgcgcgcg aggagaaact agaggaaggc aaaggcggag ccgcttccct
      661 gcgcgtgatc gccgaccacg cccgcgccac tacgttcctg atgacggacg gcgtgcttcc
      721 gtcgaacgac ggacgcgggt atgttttgcg gaaaataatt cgacgcgcca ttcgtcacgg
      781 aagactttta ggcgctacta aaccgttcct ttcctatacc gttcaaactg ttcgcgagtt
      841 aatgggccgg gcgtatccgg agctgacaga atccgcagcc gctcgggtgc cagaaattgt
      901 tttgtccgaa gaaaagcgct ttggcgcaac tctggatgcg gggctaataa agctgactga
      961 ggaactgcgt ccgttgctcc agttaagggc gcagtatgag gatgcgagca agcagctcga
     1021 ctcaacgccg ggcgggccac aaagggttga gctgtcgcag aagcgttctg aactttctcg
     1081 caaaatgacc tattcgggtg agttggcttt caaactgttt gacacatacg gcctgccgag
     1141 agacttcgtg gacgacgtat gtcgcgacgc aggcattccc gtgagctggg acgaatttga
     1201 tcgcgcgatg caggagcaac gcaggaaggc gcgggcttct tggaaaggga cgcacaagga
     1261 ggcggcaagt ccggcttacg cgaaaattgc tgagacctat aagaccgagc cgggttttta
     1321 ctacggcacg cacgctaagg actgccggat tgaagccatc atcagcaaag cgggtgcagt
     1381 caatgaactg aaggccggtg agtcggcaga agtagttctg gatcgcaccg cgatttacgc
     1441 cgaatcgggc ggacagattt tcgataccgg tgcgctttac aacgactcgg agacgcagct
     1501 gctcgccgaa gtttccggtg cgtactatcc cgtggcggga ttggtggcgc accgcgtcag
     1561 cgcgaaagaa gaactgcgtg tgggcgatcg tgtcgcggtc gtggctgacg ctgaccgccg
     1621 cgcgcgtgtg atgcgcaatc acactggcac gcacctggtg cacgcagcgc tacgcaacat
     1681 tctcggcacg cacgtgaagc aggccggttc gctgaacgcc cccgaccggc tacgcttcga
     1741 cttttcgcac ttcgctcccg tgtatgcaga agagctgcgc gacatcgaac aacaggtcaa
     1801 cgaagagatt cgtctgaaca cggaaatcga aaccaacgtc acgacgctgg acgacgcgct
     1861 agcttccggt gcgctggcct tttttggcga taaatatccc gagcacaacg ttcgcgtggt
     1921 gaccattccc gatccgcacg cgccgcgtgg cttctattca aaggaacttt gtggcggtac
     1981 gcatgtgaag cgcatcgggg acattggcgt cctgaaaatc gtcagcgaag aatccgtcgc
     2041 cgccggcgtt cgccgcgtgg aagccatcac cggcatcggc gcgctggaac attaccagca
     2101 tcaggccagc gtcctggcct ccgtcgcgaa acaactgaac gtcggcgaag atgccctcct
     2161 ggctcaagtc gaaaagctaa tccaaactgt ccggcaattg gaaaagctgt tgaccgatca
     2221 gcgccgcaaa ggggcgctga accagctgga tgacctggta gccaaggcgc aaaccatcaa
     2281 gggcaccaag gtggtggtca gcgaggtcga aaatgtggac cgggaagccc tacgccagct
     2341 ggtggacagc cttcggcaga agctcggctc aggcgtggta gtgctcggac aggccgaaga
     2401 cggcaaagtg gccctgatcg caggcgtcac caaggatcta actgctaaag tccatgccgg
     2461 caaggtgata caggcgatgg ccaaactggt caacggcaag ggcggcggta gaccggacct
     2521 tgcggagggt ggcggagagg acacaaaagc cctaaatagt actcttgggg gagtgcccgc
     2581 cctactggag ggattgctat gatcgggtcg cgccagaagc gtgtctgttc ggggagaatt
     2641 ccccggggta ggcaaagctt cacccaatgg acggcctcag cccggctgct gtcgcccgtt
     2701 gcagcaagct gtatcgacat tgaacttgca tgccttcccc aaaggcaagt caacgacgaa
     2761 acgcggccgc tctacaacgc aatgggcaaa cgaagctcca acccggtcct cgtcgccagg
     2821 gcgattctcg cctgcagcgg agcgtttttc gcagccgccg ccaatgcgca aatcgccaaa
     2881 gtctccaaca gcgatggccg gatgttcttc gtcaacgcgg atactcccac tgccttgaag
     2941 ttcgcttcgc gcaagccgcg cgctattttc ctgccggccg aagtttcctt caccggcggc
     3001 aaccgtccag cgatggactt gaatcgcgac ggcgcggaaa agctggtacg cgaggcggcg
     3061 gaacgtcaca gcgtggatcc ggcattgatt cgcgcggtaa tcgagacaga atccaactgg
     3121 aacgccggcg ccatttcccg caagggagcc gtcggattga tgcagttgat tcccaccact
     3181 gcccagcgct ttggggtcaa tgacctgtac agcccgcaac agaacgtcga tgccggtgtt
     3241 cgatatctga agacgttgct ggaacgctat aatggcaatc tg
//
LOCUS       DASTPM010000644         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_369127, whole genome shotgun sequence.
ACCESSION   DASTPM010000644 DASTPM010000000
VERSION     DASTPM010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369127"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFR42_11335"
     CDS             complement(<1..615)
                     /locus_tag="VFR42_11335"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0369798.1"
                     /translation="MSDNNLPLSRPTLGEEEIREIIDVIQSGWITSGPRAKRFEEEFS
                     RYVGARHAVAVNSCTAALHVALLAHGIGPGDEVVTSSMTWSATANMIEVTGAKPVFAD
                     VDRDTLQVAPETVAAAVTERTRAILPVHFAGQACDLDGLAANATPRGLTIIQDAAHAV
                     GTEFCGRRVGSDGITACFSFHPIKNITTGEGGMITTDSDELAEKL"
     gene            complement(612..>2102)
                     /locus_tag="VFR42_11340"
     CDS             complement(612..>2102)
                     /locus_tag="VFR42_11340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0369799.1"
                     /translation="WIIPRLNGEPFLEKPPLYWWVQSSVFALFGRATPTLARLPSALF
                     GFMSLLLTYALGRRFFSAKTCLVGCLILLSTALFSLTTHWIVVDNALLFAVTGAWALF
                     AHAEARDGATRQFVLLGMYVFLAIAFLTKGVVGLGIPALGMGVYLLWSQRLGRFFGWH
                     MVVGGAFIAGAAALWLWLLWREGGRGSLDTFLVYNQLGRFFPDAETYQGGHVRPIWYY
                     FLTTPADLLPWTPFVLLAGLSTRRNWERLPELHREGVRLCIAGTLPVLLALSLAGTKR
                     GMYLLPVFPLIALFVASWAASAEQHQGWENKLERGWEILLISCAVLSPAALFLAPSSW
                     LLWLGSVVVLYLFWYVLRHPPAAEREARLLRTAFLVCLAIGNLLITVRPFVDRFKSFV
                     PFVRQLEKYVSPATPLYAYRPDETTLGVVNFYTGRRVTEVALEQLPTLARESKPISLV
                     VRDSKRTGGNYGEILKAGISHRLLSEQVVGDDRTIRILALGDGINR"
BASE COUNT          518 a          631 c          585 g          368 t
ORIGIN      
        1 gagtttctcc gccagctcgt cgctgtcggt ggtgatcatg ccgccctcgc cagtggtgat
       61 gttcttaatg ggatggaagc tgaagcacgc cgttattcca tcgctgccaa cccgcctgcc
      121 acagaactct gttcccacgg catgagccgc atcttgtatg atcgtcaggc ctcgaggtgt
      181 cgcgttggca gccagtccgt ctagatcgca ggcttgtccg gcgaagtgaa ccggaagaat
      241 cgcgcgcgtg cgttcagtca ccgccgcagc cacggtctca ggcgcaactt gcagtgtgtc
      301 ccgatcgacg tcggcgaaca ccggctttgc ccccgttact tcgatcatgt tggccgttgc
      361 agaccaagtc atggagctgg ttacgacttc atcgccgggc ccaatgccat gcgccagcaa
      421 cgctacatga agcgcggcgg tgcaggagtt caccgccacg gcgtggcgcg cgcccacgta
      481 acgggaaaac tcctcctcga aacgcttggc gcgcggaccc gacgtaatcc agccggattg
      541 aatgacatcg atgatctcgc ggatctcttc ttctcccaga gtcgggcgtg agagtggtag
      601 attattatcg ctcatcggtt gattccgtct cccaatgcga ggatccgtat agtgcgatca
      661 tcaccgacga cttgctcgga aagcaaccgg tgggaaattc ccgctttgag gatttcaccg
      721 tagttgcctc ccgtccgctt gctatcgcgt acaactaaag agatcggttt gctttcacgc
      781 gccagggttg gcaattgctc tagcgcaacc tcggtcaccc gccgtccggt gtaaaagtta
      841 accaccccga gtgtcgtttc atcaggccgg taggcgtaga gtggcgtcgc cggactaacg
      901 tacttttcga gctgtcgaac aaacgggaca aagcttttga agcgatccac gaacggtctc
      961 accgtaatca acaggttgcc tatcgccaaa cagaccagaa aagcagtgcg cagcagccgc
     1021 gcctcgcgtt ccgccgccgg tgggtgtcgc agaacatacc aaaacaaata cagaacaaca
     1081 acggaaccaa gccaaaggag ccaactgctc ggcgccaaaa ataatgcggc tggcgataat
     1141 acggcgcagg agatcagcaa gatttcccaa ccacgttcaa gtttattctc ccaaccctgg
     1201 tgttgctccg cagaggcggc ccaggaagca acgaacagag cgatcaaagg aaaaaccggc
     1261 agcaagtaca tgcctctctt ggttcccgcc aaagaaagcg ccaataagac cggaagcgtt
     1321 cctgcgatgc agagccgaac tccttcacgg tgcagctccg ggagccgctc ccagttgcgc
     1381 cgggttgaca gacccgccag taggacgaag ggagtccatg gcaacagatc cgccggcgtg
     1441 gtgagaaaat aataccaaat cggtcgcaca tgtccgccct gatacgtttc agcgtcgggg
     1501 aaaaaacgtc ccagctggtt gtaaacgaga aatgtatcca gcgatccgcg cccgccttcc
     1561 cgccacaata accagagcca aagcgcggca gcgccggcga tgaaagcgcc acccacgacc
     1621 atatgccagc cgaaaaaacg acccagtcgc tgactccaca acagatagac gcccatgccg
     1681 agggcgggga tgcccaggcc caccacgcct ttagtcaaaa aggcgatcgc gagaaaaaca
     1741 tacatgccta aaagcacaaa ttgtcgcgtg gcgccgtcgc gcgcctcggc gtgggcgaaa
     1801 agagcccacg ccccagtgac ggcaaacagc aaagcattgt cgacaacgat ccagtgagtc
     1861 gtcagggaaa aaagcgccgt gctgagcagg atcaagcatc ccactaggca ggttttcgcg
     1921 gagaaaaaac gtcgcccgag cgcgtacgtg agaagcaaac tcatgaagcc gaacagcgcg
     1981 gaaggtagcc gggcaagggt cggagtagcg cgaccgaata gagcgaatac gctcgactga
     2041 acccaccagt acaagggagg cttctccaga aagggctcgc cgttcaatcg cggaatgatc
     2101 ca
//
LOCUS       DASTPM010000645         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_686376, whole genome shotgun sequence.
ACCESSION   DASTPM010000645 DASTPM010000000
VERSION     DASTPM010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686376"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFR42_11345"
     CDS             <1..877
                     /locus_tag="VFR42_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0369800.1"
                     /translation="VMQESLGNQLEALPGVDVVAPTVILTEPKNLIMIYGIDYKRFNA
                     LSRGFLYRSGRAFQSPDEALADDIIAQSKHLKVGDAITLLNHRFTICGIVAHGKGARY
                     FVPIRTAQDIAGADNRVSMFYVRSKGDTEKTRAEILKLDPKNRVRSTSEYVTLMSSSN
                     LPELRPFIRSMVGLGVVISFLVVLLNMHTMVLERTREVGILKALGFSRFDVVRMLLGE
                     MLVLTLLGSGLGIVLTFVTQAVLKETNPGLMILITTGWVFKALVLAVAGAALGAAYPA
                     FRAASYDPVVALAYE"
     gene            890..1690
                     /locus_tag="VFR42_11350"
     CDS             890..1690
                     /locus_tag="VFR42_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0369801.1"
                     /translation="MELTAQRLTRDKHYTATILERRDIAEDLWVIRVDPGGPFTFKAG
                     QYATLGVDHEGKRIERAYSIVSSPYEEGLEFFLELVPQGELTPKLYKLQKGDTMLCRK
                     IAKGRFTLDLRSGRTNHLLLATVTGVAPFVSYVRTLYKDWKSGDSPMPGNHKLYCIQG
                     GSRSWEFGYRQEMERVAAEVPWFRFVTTISRPWEDSAWKGETGRVEDLVRQYVREWGL
                     KPAETTGYLCGHPSMIENGREILLRAGWQKGSMFDEVYFIPGKEGGND"
     gene            complement(1697..2128)
                     /locus_tag="VFR42_11355"
     CDS             complement(1697..2128)
                     /locus_tag="VFR42_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369802.1"
                     /translation="MLLAKIALGLGGTALLAGAYVFHEGIVRVDADDSHDASHVHVWV
                     PAALVPLAIRVIPSRHFDHALSNAGPWLPTLRVLTKELRRFPEAELVEVRDGRDQVHV
                     RTHQGKLLIDVSEPGQTVHVSCPLAMMQDVAGALESKVPGA"
     gene            complement(2194..>2761)
                     /locus_tag="VFR42_11360"
     CDS             complement(2194..>2761)
                     /locus_tag="VFR42_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369803.1"
                     /translation="FIAPASFAQSPSTGGSAQTVSASSHDRWLHVRVTNPSSNEESVR
                     VNVPLELAEKVLPTINQQRLHSGKVKFDDIDCHGVDLRALAEAVRTSKDGEFVTVQNK
                     DSDVRVAKQNGHLLVHVYEKNHPKRSEVEVKVPMKVVDALFSAGKDELDLVAALHALA
                     SQGDTELVSVKDSENTVRVWLDSKNITD"
BASE COUNT          571 a          756 c          854 g          580 t
ORIGIN      
        1 agtgatgcag gaaagcctgg gtaatcagtt ggaagcgttg cccggcgtgg acgtggtggc
       61 gcccacggtc atcctgaccg agccgaaaaa cttgatcatg atctacggca tcgactacaa
      121 gcgcttcaac gcgctaagcc gcggctttct gtaccgctcc ggccgcgcct ttcagtcgcc
      181 cgatgaagcc ctggccgacg atatcattgc gcagtccaag cacttaaagg tcggcgacgc
      241 tatcaccctg ctgaatcacc gctttaccat ctgcggcatc gtagcgcacg gcaagggtgc
      301 gcgctatttt gttccgattc gaacggcgca ggatatagcc ggagccgaca accgggtctc
      361 catgttttac gtgcgcagca aaggcgacac ggaaaaaacc cgcgccgaaa ttttaaagct
      421 ggaccccaaa aatcgcgtca ggtccacctc cgaatacgtc accctgatga gttcttccaa
      481 tcttcccgaa ctccgtccat ttatccgttc catggttggc ctgggagtgg ttatcagctt
      541 tctggttgtc ctcctgaaca tgcacaccat ggtgctcgag cgcacccggg aggttgggat
      601 actcaaggca ttgggatttt cgcgttttga tgtggtgcgc atgctgctgg gggaaatgct
      661 ggttctgact ttgctgggaa gcgggctggg aatcgtgttg acgtttgtaa cgcaggcggt
      721 tttgaaggag acgaatccgg ggctgatgat tctgattacc accggctggg tattcaaggc
      781 actggtgctt gcagtggcgg gcgcggcgct aggcgcggcg tatcccgcat ttcgcgccgc
      841 aagttatgat ccggtggtgg ccttggctta cgaatagaag agtccggcga tggaactgac
      901 ggcacagaga ctcacgagag acaagcatta caccgcgacg atattggaac gcagggacat
      961 agccgaggat ttgtgggtga ttcgcgtcga tcctggcggg ccatttacgt tcaaggcggg
     1021 gcagtatgcg acgctcggcg tagaccacga agggaagcgc attgagcgcg cctattccat
     1081 cgtgtcttca ccttatgaag aaggactgga attcttcctg gaactggtcc cgcaagggga
     1141 gttgactcca aaactgtaca agctgcaaaa gggcgatacc atgctgtgcc gcaaaatcgc
     1201 caaggggcgc ttcactttgg atttgcggag cgggcgcacc aatcatcttc tgctggccac
     1261 ggtgaccggt gtggcgccat ttgtgagcta tgtgcgcacg ctgtataagg attggaagtc
     1321 cggcgatagt ccgatgccgg gaaatcacaa gctgtactgc atccagggtg gcagccgctc
     1381 ctgggaattc ggctatcgcc aagaaatgga acgggttgcg gcggaagtgc cttggtttag
     1441 attcgtgacc acgatcagcc ggccctggga agacagcgcg tggaagggcg aaaccgggcg
     1501 ggtcgaggat ttggtgcggc agtacgttcg ggagtggggg ttgaagccgg cggagaccac
     1561 aggctacttg tgcggacatc ccagcatgat tgaaaacggg cgcgagatcc tgctgcgagc
     1621 cggctggcaa aagggctcga tgtttgacga agtttacttc atcccgggga aagaaggcgg
     1681 caacgactag ggcagcctat gcgccgggaa ctttggactc cagcgcgccc gcaacatcct
     1741 gcatcatggc cagcgggcag ctgacatgga cggtctggcc cggttcgctg acgtcgatca
     1801 gtaacttgcc ctgatgtgtg cgcacgtgaa cttgatcgcg gccgtctctt acttcgacta
     1861 attccgcctc cggaaaacgc ctcagttctt tggtcagcac ccgcaaagtg ggcagccacg
     1921 gcccggcgtt ggacagggcg tgatcgaagt ggcggctggg gataacacgt atggccaggg
     1981 gcaccagtgc ggctggaacc caaacatgga catggctggc gtcgtggctg tcatccgcat
     2041 ccacgcgcac gatcccttca tgaaagacgt atgcgccagc cagcagcgcc gtaccgccga
     2101 gccccaaagc aatcttggct aacaacatgc gattcctcct gcagagttgc ggttcaactg
     2161 gccgcaagcc cgcgacctct cctctgtcgc tggttaatcg gtaatgttct tggagtccag
     2221 ccaaacgcga acggtgtttt ccgagtcctt caccgaaacg agttccgtat cgccctgcga
     2281 agccagcgcg tgcagggcag cgaccaagtc caattcgtcc ttgccggcgg agaagagggc
     2341 gtccaccacc ttcatcggta cctttacttc gacttcggag cgcttgggat gatttttctc
     2401 gtaaacgtgc accaggagat ggccgttctg cttggcgacg cggacatcag aatccttatt
     2461 ctggacggtg acgaattcgc cgtccttgga agtgcgcacg gcctccgcca gggcgcgcaa
     2521 atcaacgccg tggcaatcga tatcatcgaa cttcactttg ccgctgtgca ggcgctgctg
     2581 attaatggtg ggcaggacct tttcggccag ctccagagga acgtttacgc gaacgctttc
     2641 ttcattggaa ctcgggttgg tgacccgtac gtgcagccag cgatcgtggc tggaggccga
     2701 gacggtctgg gcggagccgc cggttgacgg gctttgcgca aaggatgccg gagcgatgaa
     2761 c
//
LOCUS       DASTPM010000646         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_398156, whole genome shotgun sequence.
ACCESSION   DASTPM010000646 DASTPM010000000
VERSION     DASTPM010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398156"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFR42_11365"
     CDS             <1..621
                     /locus_tag="VFR42_11365"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0369804.1"
                     /translation="FVDDILVASPREFGYFGEVEGSGRYAPWRNAANQAKFKSDLSRM
                     IDLILAGRKDIVTAEHANASTASREIAALPSFKVTDLAGRPLSNDQLANRVVLVEFWA
                     TWCPPCRSTLEWLGELNKRHGDNLAIVALAVESPETQIRSTVSSLSPDLRWAVTDAST
                     AQSFGDITAVPTMFLFDRSGKTVRVLYGAPPDLHEQVEKELSTLLK"
     gene            complement(1083..2051)
                     /gene="era"
                     /locus_tag="VFR42_11370"
     CDS             complement(1083..2051)
                     /gene="era"
                     /locus_tag="VFR42_11370"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU0369805.1"
                     /translation="MTNIQATKAKRAGFVAIVGRPNAGKSTLVNRLVGQKIAIVTSKP
                     QTTRNRIQGIVTRDDGQVVLIDTPGLHDAKTVLNRQMMKEVAAALEGIDALVLLVDAA
                     ETLANQGSGTQVHVDPMLIQRAKRFEGRTILALNKVDKAAKNKLLPMMEEFRKAHPFV
                     AMVPISALQGDGCDELLGEAMKLLPVGEPYFPEDQVTDQPERFLASEIVREKAIALTY
                     HEVPHALHVMVDKWEEMPKLLRIQMTLTVERPSQKKILIGRKGDMLKKVGTQARKELE
                     EILSTKIYLGLFVKVAENWRQNPMAVRELDWHTQLAALGEQKTGDE"
     gene            complement(2106..>2298)
                     /locus_tag="VFR42_11375"
     CDS             complement(2106..>2298)
                     /locus_tag="VFR42_11375"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEU0369806.1"
                     /translation="FGENADETVTTIAGLLSHVSGKVPAPGEKFDLEGFRFEVLEANQ
                     RKVLRVRAQKLTGTALPVS"
BASE COUNT          431 a          800 c          548 g          519 t
ORIGIN      
        1 ttcgtggacg acatcctcgt cgcctctccc cgtgaattcg gctatttcgg cgaagtcgaa
       61 ggttccggcc gctatgcccc ctggcgcaac gctgccaatc aggccaaatt caagtccgac
      121 ctgtcccgca tgatcgatct gattctcgcc ggcaggaaag acatcgtcac cgccgagcac
      181 gccaacgctt ccacggcgag tcgcgaaatc gccgcacttc catccttcaa ggtaactgac
      241 ctggccggcc gcccgctgag caacgatcag ctagccaatc gcgtcgtcct cgtcgaattc
      301 tgggccactt ggtgcccgcc atgtcgctcc accctcgaat ggctcggcga actcaacaag
      361 cgccacggcg acaacctcgc catcgtcgct ctcgccgtcg agtcccccga aactcaaatt
      421 cgctccactg tctcctcgct cagcccggat ctccgctggg ctgtcaccga tgcgtccacc
      481 gctcaatcct tcggcgacat caccgccgtg cccaccatgt tcctcttcga ccgctccgga
      541 aagaccgttc gcgtccttta cggtgcccct cccgacctcc acgagcaagt cgaaaaagaa
      601 ctcagcacac tcctgaaata gctctgcaat gaaaccggac gtcctggtca tcctggtccg
      661 gatgatgacc gaagcagagg tggaaactca gcgagccgag gaattggacc ggggcggcgt
      721 gtctcgtatc gcacaagcga atgcatgagc aagcgcgtat cattagcaaa tgaagcgaga
      781 actgcctgtt acgtgttgta ctgagtgcgg cggcgctggt tacaacgtga gggtcgcaaa
      841 cggtcgttgc tgcaaaacca gcggcgaact acgctgcaat ggcgttaacg caattgctac
      901 caaaccattt gattgggccg aatgtacagc ctgtcaagca accggatact accgcaacac
      961 ggaatgcccg aactgcaaag gtgtcggcta tctcttcttg ggcccgagcg ctaaagcagc
     1021 cgtttagacg cgccaatttc tggagttcca aaccgtccaa ccaagctgcg ccggcctcgc
     1081 ttctactcat cgcccgtttt ttgctctccc agcgccgcca gctgggtgtg ccaatccagc
     1141 tcccgcaccg ccatcggatt ctggcgccag ttttccgcca ccttcacaaa caatcccaga
     1201 taaatcttcg tgctcaaaat ttcttccagt tctttccgcg cttgcgtgcc aaccttcttc
     1261 agcatgtcgc ccttgcgccc gatcagaatc ttcttctgcg aaggccgctc caccgtcagc
     1321 gtcatctgga tccgcaacag cttcggcatc tcttcccact tgtccaccat cacgtgcagc
     1381 gcgtgcggca cttcgtggta tgtcagcgca atcgctttct cccgcacaat ctcgctcgcc
     1441 aaaaaccgtt ccggctggtc cgtcacctgg tcctcgggaa aatacggttc tcctaccggc
     1501 agcagcttca tcgcttcacc cagcaattcg tcgcagccgt cgccctgcag cgccgaaatc
     1561 ggcaccatcg ccacaaacgg atgggctttc ctgaactcct ccatcatcgg cagcagcttg
     1621 ttcttcgccg ccttgtccac tttgttcagg gccagaatgg tccggccctc gaaccgcttg
     1681 gcccgctgga tcagcatcgg atccacgtgt acttgcgttc cgcttccctg gttggccagc
     1741 gtctcggcgg catccaccag cagcaccagc gcgtcaattc cttccagtgc cgccgccact
     1801 tctttcatca tctgccggtt caacaccgtc ttcgcgtcgt gcaatcccgg cgtatcgatc
     1861 agcacgactt gtccatcgtc gcgcgtcaca atgccctgga tccggttgcg cgtcgtctgc
     1921 ggcttcgagg tcacaatcgc gatcttctgc cccaccagac ggttcactag tgtggacttc
     1981 cccgcattcg ggcgccccac aatcgccaca aatcccgccc gctttgcttt tgttgcttgt
     2041 atgttggtca ctcggtttct ctttgccgtc gctactccgc tgcgttcgga gtgggaactt
     2101 cttgttcagc ttacgggcag cgccgtgccc gtaagctttt gcgctctcac gcgcaacacc
     2161 ttgcgctggt ttgcttccag cacttcaaag cgaaatcctt ccagatcaaa cttttctccc
     2221 ggcgccggta ctttcccgct tacatggctc aacaatcccg cgatcgtcgt caccgtttca
     2281 tcagcgtttt cgccaaac
//
LOCUS       DASTPM010000647         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_283085, whole genome shotgun sequence.
ACCESSION   DASTPM010000647 DASTPM010000000
VERSION     DASTPM010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283085"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VFR42_11380"
     CDS             <1..1021
                     /locus_tag="VFR42_11380"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU0369807.1"
                     /translation="SGHARRMEQQTSPPDPAKEQAAAAMRHATAEEGKLDAAERRRVV
                     DAAIANLRQHYIHPEVAEKMAAELQNHQKRGDYDASTDGSAFAMLLTRDLREVSHDQH
                     LAMFYSREKTSEAPDAPSPGDLAQYKRDMEKTNCMFEKAEVLPGHIGYVKFNEFPDPP
                     TCHARMEAAMHSLQYADAIIFDVRENHGGSPEMVALIASYLFDRPTHLNDIYNRGSNS
                     TQESWTRPVKGNKLADKPAYVLTSALTFSAAEEFSYDLKMLKRATLVGETTRGGGHLA
                     SMQRIGDHFLISVPDANPINPISKTNWEGTGVEPDVKVKAADALQTAKALAEEKLRQK
                     NLPKK"
     gene            1195..1698
                     /locus_tag="VFR42_11385"
     CDS             1195..1698
                     /locus_tag="VFR42_11385"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0369808.1"
                     /translation="MEHILKDAEIAAHTKVQFHCSKCRQTTVVEIKVRADQTLVVSPL
                     PTFARSNASASNLNLPPEDGLQLPEAKSAVLTVISGPSKGDVHNLKKPRVILGREGAG
                     IVLNDQEISRHHCLLEIRENYANLKDLDSTNGTFYEEERVRAAMLRDGAEFRIGNSVI
                     RFTLVAK"
     gene            1778..>2102
                     /locus_tag="VFR42_11390"
     CDS             1778..>2102
                     /locus_tag="VFR42_11390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0369809.1"
                     /translation="MNRLSVCIITLNEEENLPRALASLKEIADEVVVVDSGSTDRTEE
                     IARAHGAVWFERAWTNYADQKNYAAECATNDWVFCLDADEELSSPLQTSLLAWKKHVP
                     EFCVYE"
BASE COUNT          510 a          596 c          637 g          359 t
ORIGIN      
        1 gtccggccat gcgaggcgga tggagcagca aacttcacca cccgatccgg cgaaggagca
       61 agctgccgcc gctatgcgcc atgcaacggc cgaggaaggg aagctggatg ccgccgagcg
      121 gcgacgcgtg gtggacgccg cgatcgccaa tctccggcag cactacatcc accctgaagt
      181 tgcggaaaag atggcggcgg agttacagaa ccatcaaaaa cgcggcgact acgacgcaag
      241 tacggacggc agcgctttcg caatgctgct gaccagggac ctgcgcgagg tcagccacga
      301 ccagcatttg gcgatgttct acagccgaga aaaaaccagc gaagctcctg acgcgccttc
      361 gcctggggat ctggcgcaat ataagagaga catggagaaa acgaactgca tgtttgaaaa
      421 ggccgaggtc ctgccgggcc atatcggcta cgtcaaattc aatgagtttc ccgatcctcc
      481 gacatgccat gccaggatgg aagcagccat gcattcgctg cagtacgcgg atgcgatcat
      541 cttcgatgtg cgagaaaacc atggcggctc tccggaaatg gtggcgctga tcgcttccta
      601 cctcttcgac cgcccaacgc atttgaatga catttacaac cgcggcagca acagtacgca
      661 ggaatcctgg acccgaccgg tgaaaggaaa caaactggcg gacaaacccg cgtacgtgct
      721 gacttcggcc ttgacgttct ccgccgctga agagttcagc tacgacctga agatgttgaa
      781 acgagccacg ctggtgggcg agaccacgcg cggcggcggc catttggcca gcatgcaaag
      841 gattggcgat catttcttga tcagcgttcc cgatgcgaac ccaataaacc ccatatccaa
      901 gaccaattgg gagggtacgg gcgtggaacc ggatgtcaaa gtgaaagcgg ccgatgcgct
      961 acagaccgct aaggcgctag cagaagaaaa gctgcggcag aaaaaccttc cgaagaaata
     1021 gcttccgaaa aatacctgcc taacaaagcg ttgcgaaaca gccttccggc agcactccga
     1081 ccgagaaggc cgaggcggta acccttgcgg caagcctgta ccgcggccca gctagtgaat
     1141 ggcccggcaa cgttataggt ttaaagagtg aaagcaaact gcgagcattg cgggatggag
     1201 cacatcctga aggacgcgga aattgcggcc cacaccaagg tgcaatttca ctgctcgaag
     1261 tgccggcaga ccacggtggt ggagatcaag gtccgagccg accagacttt ggtggtttcg
     1321 ccgctgccca cgttcgcgcg cagcaatgcc agcgcgtcca acctgaactt gccgccggaa
     1381 gacggcttgc agctgcccga ggccaagtcg gccgtgctca ccgtgatttc cggaccatcg
     1441 aaaggcgatg tgcacaattt gaagaagccg cgggtaattc tgggccgcga aggcgccggc
     1501 atcgtcttga acgaccagga gatttcgcgc caccattgcc tgctggagat tcgggagaac
     1561 tacgccaacc tgaaagacct ggattccacc aacggcacgt tttacgaaga agagcgggtg
     1621 cgcgcggcga tgcttcggga tggggcggag tttcgcattg gcaacagcgt gatccgcttt
     1681 acgctggtgg ccaagtagcc gatcgcaagc caagaaacgc cagcctgccg gaagtcgtga
     1741 gatactggcg cggactgcgg ttgctccctt tttcccgatg aatcgccttt ccgtttgcat
     1801 cattacgctg aacgaagaag aaaacctgcc gcgcgcgctg gcttccttga aggaaatcgc
     1861 ggacgaggtg gtggtggtgg attccgggag cacggaccgc accgaggaaa ttgcccgggc
     1921 gcacggcgcg gtctggttcg agcgggcgtg gaccaactac gccgaccaga aaaattacgc
     1981 cgcggaatgc gccacgaatg attgggtgtt ctgcctggac gccgacgaag agctgagcag
     2041 cccgctgcaa acctcgctgc tggcctggaa aaaacacgtg ccggaatttt gtgtttacga
     2101 ag
//
LOCUS       DASTPM010000648         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_830447, whole genome shotgun sequence.
ACCESSION   DASTPM010000648 DASTPM010000000
VERSION     DASTPM010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830447"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFR42_11395"
     CDS             complement(<1..256)
                     /locus_tag="VFR42_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369810.1"
                     /translation="MPKIKELECSRCKATVSAETPQTVCPKCAGSFYVRYDFSGFRGV
                     EHRMRISEKTPGASAGMWRYRSVLPEVQPVTLGEGWTPMLR"
     gene            396..1751
                     /locus_tag="VFR42_11400"
     CDS             396..1751
                     /locus_tag="VFR42_11400"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEU0369811.1"
                     /translation="MTQERSNSFRLLLAAGLILFFAGALLQPFFIYHTPLIALRYAGL
                     LLLAIACLQKRSLALWIFFAMLAGLELGLDAPSFAVRLRVLSDIFLRLIKVIVAPLIL
                     GTLITGIAGHGDLRKVGRIGWKSIVYFEAVTTVALFIGIFAINLTKAGEGLILTATAA
                     AQTPPDTAPLRWTDFLLHIFPENIVKSVAENQILQVAVFAIIFAIALTRVRADLQKPI
                     LNFAESLTQVMFSFTNLVMYYAPVGIGAAMAYTVGHMGIAVLLPLVKLILTAYGALLA
                     FLLLVLLPIALLARIPLKRFLSHVAEPATIAFATSTSEAALPRAMECMEAFGVPRRIV
                     AFVIPAGYSFNLDGSTLYLALAAIFVAQAAGIHMPWSQQLLMVLTLMLTSKGIAGVPR
                     ATLLVLLASAASFHLPTEPVFVILGVDALIDMARTSLNVTGNCLASAVIARWENELPT
                     D"
     gene            complement(1820..3286)
                     /locus_tag="VFR42_11405"
     CDS             complement(1820..3286)
                     /locus_tag="VFR42_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEU0369812.1"
                     /translation="MAQCDSLKPEATLWVRLLQPVSSYSGKAGDRVQAMVIESPACDG
                     TEAIGAGTLVEGKIKAASRVGMGLVHETATLRVDFDYLVTKDGKEIELSSQVEAIDNA
                     RETVKNGVIHGVNATDTPQGRITSRLKHLPTWNPYSDLTLVAFRAAFPVFPEPEIYLP
                     RGTDLKLGLATELQIPEELRGVAQASSPDEIERATMEITAPTLPERTTTRHGRAADFV
                     NVALVGSREQMDQAFQAAGWEHGDRTSTRSVMREIHAFLAFKSYPRAPISQQLVDREP
                     ASATWEKTLDSYEKREHLRVWARNDVIGGQTVWLGAMTRETGATLSVRQHKFIHHVEA
                     DLDEGRGMLVRDLSLAGCVAAVYFVDRPQMEHLAMNATGDAMRTDGSLAVVQLKDCEN
                     AVFERQAADAAALATSRPHSKFARYVRMQVLSFKSDVVRGNIVYGVFDLTRMAIRARR
                     NHTQRVELARQVGTTPEPRAHVASGASSPVLSWSDGGE"
BASE COUNT          671 a         1331 c          870 g          888 t
ORIGIN      
        1 tgcgcagcat cggcgtccag ccttcgccca gcgtcaccgg ctgcacttcc ggaagtacgg
       61 atcggtaccg ccacatccct gccgacgctc caggcgtctt ttctgaaatc cgcatccggt
      121 gttccacgcc ccgaaatccg ctgaagtcat accgcacata aaacgatccg gcgcatttcg
      181 ggcacaccgt ctgcggcgtc tccgccgaaa cggtcgcctt acaccgcgaa cactccaact
      241 cttttatttt tggcatccct gcaagatatc agcgcggtat ttgcactcgc aagttggacc
      301 tcgtcgccga gcgaagcttc tgccattcgc tctcgtaatt ccggcttctc ggctatgatg
      361 tcggttcgtt ttcgtcgtcc tggagaggct tttcggtgac ccaagaacgg tccaattcgt
      421 tcaggcttct tcttgccgct ggcctgatcc tcttttttgc cggcgcgctc ttgcagcctt
      481 ttttcatcta ccatacgccc ctgatcgcgt tgcgctacgc tggcctgttg ttgctcgcga
      541 tcgcttgtct tcagaaacgc tctcttgccc tctggatttt cttcgccatg ctcgcgggcc
      601 tggaactcgg tctcgacgca ccgtccttcg ccgttcgact cagggtactc agcgacattt
      661 tcctgcgcct catcaaagtc atcgtcgctc cgctcattct aggaacgctc atcaccggca
      721 tcgccggcca tggcgacctc cgcaaagtcg gccgcatcgg ctggaaaagc atcgtttact
      781 tcgaagctgt caccaccgtc gcactattca tcggaatttt tgccatcaat ctcacgaagg
      841 caggcgaagg tctcattctt accgcaaccg cagcggcgca aactccacca gacaccgcgc
      901 cccttcgctg gaccgacttc cttctccaca tttttccgga aaacatcgtc aagtccgtag
      961 ccgaaaatca aatcctgcaa gtcgccgtct tcgccatcat tttcgccatc gcgcttacgc
     1021 gggtccgcgc agacctgcaa aagcccattc tcaatttcgc cgaatcgctc acgcaggtca
     1081 tgttcagttt caccaacctc gtcatgtact acgcccccgt cggcatcggc gcggcaatgg
     1141 cctacaccgt tgggcacatg ggcatcgcgg tactgcttcc gctcgtcaaa ctcatcctca
     1201 ctgcatacgg cgccctgctc gcttttctgc tgctggtgct tttgcctatc gcgctattgg
     1261 cgcgcattcc tctaaaacga ttcctctctc acgtcgccga acccgccacc atcgcttttg
     1321 ccaccagcac ttccgaagcc gccctcccgc gcgccatgga atgcatggaa gccttcggcg
     1381 tgccacgccg catcgtcgcg ttcgttatcc ccgccggcta cagctttaat ctcgacggct
     1441 cgacccttta cctcgccttg gctgcaatct tcgtcgcgca agccgccggc attcacatgc
     1501 cgtggtccca gcaactcctc atggttctca ccctgatgct caccagcaaa ggaatcgctg
     1561 gtgtaccccg tgccactctg ctcgttcttc tagcctcggc cgccagtttc catctgccca
     1621 cggaacccgt cttcgtaatt ctcggcgtcg acgcgctcat cgacatggcc cgcacctctc
     1681 tcaacgtcac cggaaactgc ctcgccagcg cagtgatcgc ccgctgggaa aatgaactcc
     1741 ccaccgactg aacacacgtt agttaacgct tgtcgccaac gaacctacaa ccctgattct
     1801 tctttgaaat tgcttcagcc tattcgccgc catcagacca gctaagcacc ggtgagctcg
     1861 cgcccgacgc cacatgcgcc cgcggctccg gagtcgttcc gacctgccga gccagctcca
     1921 ctctttgcgt atgattccgc cgcgcacgaa tcgccatccg ggttaagtca aatactccgt
     1981 aaacaatgtt gccgcgcact acatcgctct tgaagctcag aacttgcatg cgcacatacc
     2041 gcgcgaattt cgaatgcggg cgcgacgtcg cgagtgccgc cgcatctgcc gcctgtcgct
     2101 caaacaccgc gttctcgcaa tcttttagtt gcactaccgc cagtgaacca tcggtccgca
     2161 tcgcatcgcc cgtcgcattc atcgccaggt gctccatctg aggacggtct acgaaataca
     2221 ccgccgccac gcaccccgcc aggctcaaat cgcgcaccaa catgccgcgg ccctcatcca
     2281 ggtccgcctc aacgtggtga atgaacttgt gctgccgtac cgaaagcgtc gcgcccgttt
     2341 cccgcgtcat cgctcccagc caaaccgttt gcccgccaat cacgtcgttg cgcgcccaca
     2401 ctcgcaagtg ctcgcgcttc tcgtagctgt ccagggtttt ttcccacgtc gcgctcgctg
     2461 gctcgcggtc caccagttgc tgcgaaatcg gcgctctcgg ataactcttg aacgccagaa
     2521 acgcgtgaat ctcgcgcatc accgatcgcg ttgacgtccg gtcgccgtgc tcccagcccg
     2581 ccgcttggaa cgcctggtcc atctgttccc gtgagcccac cagcgcaacg ttcacgaagt
     2641 ccgccgctcg tccatgtcgt gttgtggtac gctccggcaa agttggcgcc gtaatctcca
     2701 tcgtcgcccg ctcgatttca tccggcgaac tcgcctgcgc cacgccgcgc agctcttccg
     2761 gaatctgcag ttccgtggcc aggccaagtt tcaaatccgt cccacgcggc aagtaaattt
     2821 ccggttccgg aaaaacggga aaggctgcgc gaaaagccac cagcgtcagg tcgctgtagg
     2881 gattccacgt cggcaggtgt tttaatcggc tggtgattct tccctgcgga gtgtccgtcg
     2941 catttacgcc gtgaattacg ccgttcttta ccgtctcgcg cgcgttgtcg atggcctcca
     3001 cttgcgacga tagctcaatc tcttttccgt ctttcgtcac cagataatcg aagtccactc
     3061 gtagcgtcgc ggtttcatgt actaacccca tacccacgcg gctcgccgct ttgatttttc
     3121 cctccactag agtccccgcg ccgatcgctt ccgtaccatc gcaagctggc gactcgatga
     3181 ccatcgcctg cactctatct cccgctttgc cgctgtaaga cgacaccggc tgcagcaaac
     3241 gcacccacaa tgtcgcttcg ggttttagcg aatcacactg cgccactgcc gatggacacc
     3301 agccgcaaac tagcaaagcc agaacgaccg tcgaagcaca atttgcgcgc cagcgcggtt
     3361 gccgccctgt gaagatagaa agatgggtca acggtcttct cacacttcca ttctaggttt
     3421 cgttgcttgg atgcgcactt catttccgca ggttttctct tgccgtcacc tgttcctacg
     3481 aacagttcgc ccgtctgtac tcaattgctt gctttccgct ttccgctatc cgtttttttg
     3541 catcgacagg cctgctctct ccagccgcct cgcccttgct ctttttttca aaaaccgcga
     3601 tgcttatccg tcgcccaccg ccacatccgt aacgatgcgc tgatctggaa tcgattttga
     3661 ccttcacaca ccgttcccgc ctgatgcggc ttcccgtcta tgcactgctt ctgtttgcgc
     3721 tcactcccgc ggtcgcggac gacatcaaga gcatcgcccc
//
LOCUS       DASTPM010000649         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1032554, whole genome shotgun sequence.
ACCESSION   DASTPM010000649 DASTPM010000000
VERSION     DASTPM010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032554"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1280
                     /locus_tag="VFR42_11410"
     CDS             165..1280
                     /locus_tag="VFR42_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_035718769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEU0369813.1"
                     /translation="MNTEQKIIKNKVGLLKLSEMLGSVSEACKVMGYSRDSFYRFKEL
                     YDKGGELALREISRRKPCPKNRVEDHVEKAIMEMALEKPALGQMRVANELTKRGLFVS
                     ATGVRSVWLRHDLETFRKRLKALEAKAAQEHIVLTEEQLRAVERAREEKEAHGEIETA
                     HPGYLGAQDTYYVGTIKSVGRIYQQTFLDTYTKVAFAKLYDRKNALVAADMVNDRVLP
                     FFEEQKVPLLRVLTDRGTEYCGQREHHEYQLYLAVENIDHSRTKARHPQTNGMCERFH
                     RTIQEEFYATAFRKKLYQSLDELQADLDDWLREYNLTRTHSGKYCYGKTPMQTFLDSA
                     HLAREKMLDSFSNAAPEPERSPAQRSVGGGAVADAAA"
     gene            complement(1938..3056)
                     /locus_tag="VFR42_11415"
     CDS             complement(1938..3056)
                     /locus_tag="VFR42_11415"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0369814.1"
                     /translation="MKKNTTELENSRRLVLRKNKQRELTIGVDLGDKTSRYCVLDREG
                     NVLFERSAATTKKGLAQAFASMGASRIAMEVGTHSPWVKRLLESWNHEVIVANAHRVK
                     LITESTRKDDRLDARKLARLARVDPELLSPIRHRSEGAQAHLVMIRARAALVEARTKL
                     VNSARGVTKSFGERLASCDPDQMRVKCLVGLPSSLQSALQPLVTAVESLTRQIHEYDN
                     KIAQIARSGYPETARLRQVTGVGELIALTYVLTLEDPQRFRRSRDVGGYLGLRPKRRD
                     SGASRPQLRITKEGDVYLRMLLVQGAQYILGWHGVDTDLRRWGLKLAARGGKNAKKRA
                     VIAVARKLAVLLHRLWVTGARYEPLLHSQIASSLQVAV"
BASE COUNT          686 a         1024 c          873 g          689 t
ORIGIN      
        1 ttcgacagag ccctgagcga gaatgacatc gccgcactgt acttggcggg gtccgaacaa
       61 tagttgcgat atagaataac ctcgcgcggc aataatctgt actagtctca acttgatctg
      121 acatttgata gactgcgcgg aagcgcggtc aggagaatgt cagcatgaac accgaacaga
      181 agatcatcaa aaacaaagtg ggcttgttga agctcagcga aatgctcggc agtgtctcgg
      241 aagcctgcaa ggtgatgggt tattccaggg acagctttta ccgctttaag gagttatacg
      301 acaaaggtgg cgagctggcg ttgcgcgaga tcagccgacg caagccctgc ccgaagaacc
      361 gggtcgagga ccacgtcgag aaagccatta tggagatggc cttggagaag ccggctctgg
      421 gccaaatgcg cgtggcgaac gaactgacca agcgtggcct cttcgtttct gccacgggcg
      481 ttcgcagcgt ctggctccgt catgatctgg aaacgtttcg caaacggctg aaggcgctcg
      541 aagccaaggc cgcccaagaa cacatcgttc tgaccgagga acaactccgc gccgtggagc
      601 gggcgcgcga ggaaaaggaa gctcacggcg agatcgaaac tgcgcatcct ggctacctcg
      661 gcgcccaaga tacgtactac gtgggcacga tcaagagcgt gggccggatc taccagcaga
      721 cgttcctcga cacgtatacc aaggtcgctt ttgccaagct ctacgaccgc aaaaatgctc
      781 tggtggccgc cgacatggtc aacgaccgtg tgctgccgtt cttcgaggag cagaaagttc
      841 cgctgctgcg cgtgctcacc gatcgcggta cagaatactg tgggcagcgc gagcatcacg
      901 aataccagtt gtacctggcg gtcgagaata tcgaccacag ccgcaccaag gcccggcatc
      961 cgcagaccaa cgggatgtgc gagcgcttcc atcgcaccat ccaggaagag ttctacgcca
     1021 cggccttccg caaaaaactc tatcagagcc tcgacgaact acaggcggat ctcgatgact
     1081 ggctccggga gtacaatctg acacggactc attcgggcaa gtactgttac ggcaagaccc
     1141 cgatgcaaac tttcttggac tcggcgcacc tggcgcggga gaaaatgctc gactcgttct
     1201 cgaacgcagc gccggagccg gagcggagcc ctgcgcagcg gagcgtaggc ggaggcgcgg
     1261 tcgctgatgc ggctgcttga tcaaccaact atgtcagatc aagtctgagc tattacacat
     1321 aattgctact gctccttctt gggggaaagc ttactcgatg aaccccgatc catccggctc
     1381 aatggcggac gcgccgaacg tggtcctgac gttcactgct acggatggac accgagatct
     1441 cgtgctgacg ctatgatgtg ttggtcgaac ccttcgcagc taagagcgca gttcgcgcct
     1501 ggaatactgg gtccccaggg gcgacttcca tgagaaggcc gcaacgtgtc aatagttttt
     1561 taagctactc taacgctgcc cgcccttggc cgaccaacgg gaggcgccag catcgtctca
     1621 aattccgcct ttctaagaac gctttttgtc cgtgccacca gcagcgtgca tcgccgacca
     1681 acgggaggcg cagcagaggc cgcactttgt gtgctggccc tgacatccta tttccaattc
     1741 cgccgattcg ccctgcttcc attcgcactt tgtactggag cctgtggctc cgtgccagtt
     1801 ggggtcctcg attctaatcg ggtgctgcta tctcggaaac ggcctttgag caccgtatag
     1861 gcgccttggc ttgtcgcctc tgcgcagtca cccgaactca gcggtcttca gaaatcatgc
     1921 tgcactctct cgtctaccta aaccgctacc tgtaaactcg aagctatctg gctgtggagc
     1981 aatggctcgt agcgcgcgcc tgtgacccac agccggtgta acagtaccgc cagtttgcgc
     2041 gccaccgcaa tgaccgcccg cttcttcgca tttttgcctc cgcgcgctgc cagtttcagc
     2101 ccccagcgcc gcagatccgt gtccactcca tgccagccta aaatgtattg tgctccctgc
     2161 accaacagca tgcgtaaata cacatcgcct tccttggtga tccgcaactg cggccggctt
     2221 gcgcctgagt ctcgccgctt cgggcgtaat cccaggtaac cacccacatc ccggctgcgc
     2281 cggaatcgtt gcggatcttc cagcgtcagc acatacgtca gcgcgatcaa ctcgcccact
     2341 cccgtcacct gccgtaagcg tgccgtctcc ggatacccgc tccgggcgat ctgcgcgatc
     2401 ttgttgtcgt actcatgaat ctgccgggtc agagattcca ccgccgtcac cagcggctgc
     2461 aacgccgatt gcagcgaact cggcaaaccg actaaacact tgaccctcat ctggtcggga
     2521 tcgcaactcg ctagccgttc gccaaacgac ttcgtcactc cgcgcgccga gttcaccagc
     2581 ttcgtccgtg cttctaccag cgcagcacgg gctcggatca tcaccaggtg tgcctgcgcg
     2641 ccttcactgc ggtgccgaat aggcgacaac aactccggat ctacgcgcgc cagccgcgcc
     2701 agcttgcgcg cgtccaggcg gtcatccttg cgcgtactct ccgtaatcaa cttcactcga
     2761 tgtgcgttgg ccacgatcac ttcgtgattc cagctctcta acagccgctt cacccaaggc
     2821 gagtgtgttc ccacttccat tgcgatgcga ctggcaccca tgctggcaaa agcttgcgcc
     2881 aaaccttttt tggttgtcgc cgcgctgcgc tcgaacagca cgttgccctc gcggtcgagc
     2941 acgcaataac ggctcgtctt gtctccaagg tccactccaa tggtcagctc gcgctgcttg
     3001 ttcttccgca acacaagccg tctcgagttc tccaattcgg tggtattctt cttcacggcc
     3061 ggttttcctt tctgcaactc gagtgcgttg accattggga gcataacgca tcccgctgga
     3121 agccggcctt ctcatttcat ctcgaaatcg atgcgaaatg gttaacaacc ttgcaggttt
     3181 ccggagtatc gactccggaa tagctctttt gcgccctgca ggtggtgctc cacaacaacg
     3241 cataagtcaa gtcgaagatc caccatttat cc
//
LOCUS       DASTPM010000650         2753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_625855, whole genome shotgun sequence.
ACCESSION   DASTPM010000650 DASTPM010000000
VERSION     DASTPM010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625855"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFR42_11420"
     CDS             complement(<1..190)
                     /locus_tag="VFR42_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU0369815.1"
                     /translation="MKSKVHPLAWIIAAVVRAVTGVQVHWQGCVPEDRQRIYFANHSS
                     HLDFVVLWSALPGEVRAKT"
     gene            complement(187..807)
                     /locus_tag="VFR42_11425"
     CDS             complement(187..807)
                     /locus_tag="VFR42_11425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003213515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0369816.1"
                     /translation="MPSIYGLKPRFQALLRPMVRQLAAAGVTANQVTVAAAILSLATG
                     ATIAWRGGGKTLLLLPVVLFVRMALNAIDGMLAREHNQKSALGAILNELGDVIADAGL
                     YLPLGIVPGFDPRLLTCVVLLSILTEMTGVIAVQIGASRRYDGPFGKSDRAFVFGLLG
                     LLLGLGLKIERWLLAILALMILLLILTIGNRARQALREARAEPSSK"
     gene            complement(842..1327)
                     /locus_tag="VFR42_11430"
     CDS             complement(842..1327)
                     /locus_tag="VFR42_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369817.1"
                     /translation="MPDVLLSLVAIVSFRRVWRHMAAAIAGAVCGGAVMFFWAQENPA
                     KAYAAVRKVPFITERMFAQVEAGYKKNGIAAVYLGPMSGTPYKIYAVEAPRFFRATPF
                     LLTTIPARGWRFVLVGLFAAACAAILRRRWNKSVRELTILHALAWALFYISYWTRISM
                     N"
     gene            complement(1510..2172)
                     /locus_tag="VFR42_11435"
     CDS             complement(1510..2172)
                     /locus_tag="VFR42_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEU0369818.1"
                     /translation="MIDVRISQQLKAKCAKATLGCLEAEVETGATPAALDELLRGYEE
                     RIAKLADPRSILEAPAILATRSGYKALGKDPARYRGSAEALLRRLIAGKGLPRINSVV
                     DVINMVSVESRLPIGLYDLAHVEGAIEFRAGRAGETYKGIGKYDLNLEGLPLFADAKG
                     AHGSATSDSERTMVTENTKRVLALIVSFGGPNGLEQWNQRLGELLATHSSAKAVKSRI
                     VT"
     gene            complement(2213..2551)
                     /locus_tag="VFR42_11440"
     CDS             complement(2213..2551)
                     /locus_tag="VFR42_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0369819.1"
                     /translation="MTSSQSRSSGIKAAAGLACVFLMCVAAKSDDKSASLYKAKCAVC
                     HGAAGKGDSPAGKSMGVVSFSDPKVAGKSDAELKEAIEKGKNKMPAYGKSLKSDEIQG
                     LVAYIRSLKQ"
BASE COUNT          585 a          863 c          723 g          582 t
ORIGIN      
        1 gtgttttcgc gcgcacctct ccgggcagcg cggaccacaa caccacaaaa tccaggtgac
       61 ttgaatggtt ggcaaaataa attctctggc ggtcctccgg aacacacccc tgccagtgca
      121 cttgcacgcc ggtgacagcg cgcaccaccg ccgcaatgat ccaagccagc ggatgcactt
      181 tgcttttcat ttcgatgacg gctcggctcg ggcttcacgc aatgcctggc gcgctcgatt
      241 ccctatggta aggataagca gcagaatcat cagcgcgaga atcgctagca gccagcgctc
      301 gatctttaaa ccgagaccca gcagcaaccc cagcaagccg aatacaaatg cccgatcgct
      361 ttttccaaac ggtccgtcat aacggcgcga cgcgccaatc tggaccgcga ttactccggt
      421 catctccgtc aagatggaaa gcagcacaac gcaagtaagc agccgaggat caaaacccgg
      481 aacgatgccc agcggcaggt acagcccggc gtcggcgatc acgtcgccta gttcattcaa
      541 aatcgcaccc agcgcacttt tctggttgtg ctcgcgcgcc agcatgccgt cgatggcgtt
      601 taacgccatc cgcacaaaca aaactacggg aagcagcagc agagtttttc cgccgccgcg
      661 ccaggcgatg gttgcgccag tagccagtga aagaattgcc gctgcaaccg tgacttggtt
      721 cgcggtaacg cccgctgcgg ccaactggcg caccatgggc ctgagcagcg cttgaaatct
      781 tggtttcagc ccgtagatgc tgggcatagg gcttcctccc gcaaccgcgt agatgcccac
      841 ttcaattcat cgagattctt gtccaataag aaatgtagaa aagcgcccag gccagcgcat
      901 gcagaatggt aagttcccgc acgcttttat tccatcgcct gcgaagaatt gccgcgcacg
      961 cggctgcaaa gagaccgacc aggacaaatc gccagccgcg cgccggtatg gtggtcaaca
     1021 gaaacggtgt tgccctgaaa aacctgggcg cttcaaccgc gtatatcttg tacggcgttc
     1081 ccgacatcgg ccccagatag accgccgcta ttccgttttt cttatagccg gcttcgacct
     1141 gcgcgaacat ccgttctgtg atgaagggaa ccttcctgac ggcggcgtag gctttcgctg
     1201 gattctcttg cgcccaaaag aacattacgg ctccgccgca aacggcgcct gcgatggccg
     1261 ccgccatgtg ccgccatacg cgccggaagg agacaatcgc aaccagcgaa agcaaaacgt
     1321 cgggcacgat gaagaaaaat gtagcttcga agaagcccca aaaaaacgat aaggctagtc
     1381 caccggaagc cgcgaggaaa tccgctccgc cgaagaacgg cagatcctgt ttggatgcac
     1441 gggcctcgat tgcctggtga ccctcggcgt ggctcgttgg cgggaatttt accgcacgta
     1501 acggcgcggc taggtgacaa ttcgcgattt tacagccttt gcggaagaat gtgttgccag
     1561 cagttcaccc aggcgctggt tccactgttc cagtccgttg ggtccgccga aggaaacgat
     1621 gagggccaga acgcgtttgg tgttttccgt caccatggtt cgctcggaat cgctcgttgc
     1681 gctgccgtgc gcgcctttcg cgtcggcaaa cagaggcaaa ccttccagat tcaaatcgta
     1741 ttttccgatt cctttgtagg tttcgcccgc acggcccgcg cgaaattcaa tcgcgccttc
     1801 cacatgtgcc aaatcgtaaa gcccgatggg cagacggctc tccaccgaaa ccatattgat
     1861 cacgtccacc accgaattga ttcgtggcaa acctttgccg gcgatcaacc ggcgcagcag
     1921 cgcttccgcc gagccgcgat accgcgccgg gtctttcccc aacgctttgt atccgctgcg
     1981 cgttgccaga attgccggtg cttccagaat cgagcgcggg tcggccagct tggcgattcg
     2041 ttcttcatag ccgcgcagca gttcatccag cgccgcgggc gttgcgccgg tttccacttc
     2101 cgcttccagg caacccagcg tcgccttcgc gcattttgcc ttcagttgtt gggaaatccg
     2161 gacgtcgatc atgcgcgcgg catcccggtt ttctcgaacg ctctcgccgc tttcactgct
     2221 tgaggctccg gatgtacgcc accagtcctt gaatctcgtc ggacttcaga ctttttccat
     2281 aagccggcat tttgttcttg cccttctcga tcgcttcctt cagttcggcg tcgctcttgc
     2341 cggcaacctt gggatcggaa aaactcacca ctcccatgct cttgcccgcc ggcgaatcgc
     2401 cttttcccgc agcaccgtgg cacaccgcgc atttcgcctt gtacaacgaa gcgctcttgt
     2461 cgtcgctttt cgctgcaacg cacatcagaa aaacacaagc caagcccgcg gctgctttga
     2521 ttcccgagct tctcgattgc gaggacgtca ttgtcgcgcg acctccgtct cgcgccatca
     2581 taatacggag gccctccgag cgtggactgt gtaattcgat ttgttcggta ccagaaagta
     2641 ttagaagtcc taagattgaa cgtatccgtt tgtgaagcac tttaccgctg gcaggacctg
     2701 gctcagattc tcggttggtc cgcaagcact gcagtacgca tgacgaaacg cta
//
LOCUS       DASTPM010000651         5591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1032632, whole genome shotgun sequence.
ACCESSION   DASTPM010000651 DASTPM010000000
VERSION     DASTPM010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032632"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VFR42_11445"
     CDS             complement(<1..1284)
                     /locus_tag="VFR42_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU0369820.1"
                     /translation="MNLTRALDVALPDIPAREILQRVPRIEPSATFREHVEDGERIVR
                     VYFPSTGLMYRITWAQWSLARLFDGNRSYATIAELYSQQMGTFYDEQTIFEFASELQT
                     AGFWYQTPQEKNVLLLLQSKEERRQHLKAKSRFSDLSMITFPAFNPDGFLNWLYPKTR
                     FIYTKWFTALTSIAFLFTLGLTLGHWSEIGRDTAEFYSFTDKTLADIAILYLLGIFVV
                     AVHEFAHAHACKHAGGRVPAMGFALIFLTPAFYTDTTEGAVMGTRAQRLVISLAGIWA
                     ELMVCSVATPIWWLTAPDTVIHEGAYFMMMLTGIVSLVVNWNPLIKLDGYYMLCEIIG
                     IAELKEDSTAYLSAWVKKHVWRLPVEVPYVPKRRRPFFAVYALLSGAYSYLVLTVVAR
                     FAGNVSRNFSPEWAFIPQYGVALVVFKSRIRLLVNF"
     gene            complement(1281..2201)
                     /locus_tag="VFR42_11450"
     CDS             complement(1281..2201)
                     /locus_tag="VFR42_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0369821.1"
                     /translation="MKPRCKALLATMFVAALSGCGDQKESPSAAQAASAPEKNSATAA
                     TAPTHNSGDASGNVLTITAPLIVEHQVDVTAQREGIVAKISADTGTRVAAGTLLAQLD
                     NRQLTANLEAARAKTRSIAADLKNWQAEAEVLKADYVRAQRLYAEKLIAEEQLQHAKY
                     KAESDQWDILRVKEQLNTAKEEEHSLELELEKTRIMAPFAGLVARRYVREGQSVGKAD
                     RLFWVTAEGPLRVRFTLPEKFIRRVKKGQHLPLTRSDLVAEKHDVKIVEVSPVVDPSS
                     GTIEVLAELVGECGELRPGMTVTVHVPDTQ"
     gene            complement(2222..4612)
                     /locus_tag="VFR42_11455"
     CDS             complement(2222..4612)
                     /locus_tag="VFR42_11455"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0369822.1"
                     /translation="MKFDLNFLAAQLLAAQELAPRARRIAQAVAEAVTGAFVNIYTIG
                     TIDGREAWVVRASAGGESVMAGHAPIGEGSLGELQARQTALLFSGADLSREDYAHLDV
                     RRTLRSLAYLPLAQGAALTGAIEILSFDSVLIPEQLQALQPLAQISATALAAAQKYEA
                     ERNDSLASVTRLTQLYDLERSFSSTLEMDELLPIIGSKFHEVMECAAINLWLLRGDES
                     IELMQQSGSDATTPAGSVQRPGEGIAGQVSDDGEAVLIANADDPRLTTRNATVHGDGG
                     VRTLLVVPILDQGALVGVVEAINKLTGAPFDDDDLFALTTITETASSALHNASLLMAE
                     RKVEVLETLVTVSHEITSTLNLERMLQTIVNAPQAVIPYERAAIALQQRGRFKLSAVT
                     GVTQVNPDAPDLAPLNGVLQWAALAEEIVNVRQHDEQIDTDREETRAKFRQYFAETGM
                     RGFYAIPLNDDTGRVGILSLESSDPDFLTSVHLELLQVLAGQATVALRNAQMYKEVPF
                     ISVLEPVLERKRRFLAMEKTRRTLYTVAAMAALVFLVIFPLPMRLAGDAVVAPVRSAQ
                     VQPEFEGVIGKVFVHEGEPVKRGQAIAEMESWDRQTALAAAQARYQTALLQMNHALAS
                     NDDAEAGIQRVQADYWKTEVTNAQQLLDRAELRSPIDGLVTTPHIDTFVGRRLQFGDA
                     FADVVDASTAILDVAIDDYDVSLLRAGQRAAIKLNGYPTRTFHGTVTVVSPKGEAQGD
                     SRVFFARVAVDNASGLIRTGMEGRAKISTGWRMSGYVLFRRPAIWLYSRIWNWFGW"
     gene            4795..5136
                     /locus_tag="VFR42_11460"
     CDS             4795..5136
                     /locus_tag="VFR42_11460"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0369823.1"
                     /translation="MEIDVRTQDQVKVIKLRGKLHLGPALDRTNDTIKDLLNSGEARL
                     VVDLEEVPMIDSSGIGLLVRHLTMAKQRGGALKLLKPSKFALQTLKVVRVLNLFEVFE
                     DQQLAVASFQK"
     gene            complement(5159..>5591)
                     /locus_tag="VFR42_11465"
     CDS             complement(5159..>5591)
                     /locus_tag="VFR42_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU0369824.1"
                     /translation="MAGALTGTSTVTSYIESAAGVAAGGRTGLGNLLIAAFFGVAMFC
                     APLVAAVPAYATAPALILVGALMCGAISRVKWDDFTEAFPAFLAVILTPLTFSIATGL
                     SLGLLSFTFLKLGAGRHKEISPLIWILSVLFLFRYAFLGSE"
BASE COUNT         1141 a         1641 c         1634 g         1175 t
ORIGIN      
        1 aaaattcacc agcaggcgta ttcgagattt gaaaaccacg agcgcaaccc cgtattgcgg
       61 gatgaaagcc cattcgggac tgaagtttct agagacgttt cccgcaaacc tggccacgac
      121 ggtgagcact aagtagctgt acgctccgga gagcagggcg tagaccgcaa aaaacggtcg
      181 tcgccgtttg ggtacgtagg gcacttcgac gggcagcctc cagacgtgct ttttcaccca
      241 ggcggaaaga tacgctgtcg aatcttcctt aagttccgcg atgccaatga tctcgcaaag
      301 catgtagtag ccgtcgagct tgatgagcgg gttccaattc accacgaggg acacgatgcc
      361 tgtcagcatc atcatgaaat acgcgccctc gtgaatgacc gtatctggag ctgtaagcca
      421 ccagatcgga gtggcaaccg aacagaccat gagttccgcc cagatgccgg cgagcgaaat
      481 taccagtcgt tgcgcgcgtg tccccatcac cgcgccttcc gtggtatcgg tgtagaacgc
      541 tggggtcaga aaaatcagcg cgaaacccat tgcgggaacg cggccccccg catgcttgca
      601 ggcgtgagca tgtgcaaact catgaaccgc caccacgaag attcccagaa gatagagaat
      661 cgcaatgtcg gctagcgttt tatcagtgaa actataaaac tcagcggtgt cgcgaccgat
      721 ttcggaccaa tggcccagcg tcagacctaa ggtaaagagg aaagcgatgc tggttagggc
      781 tgtgaaccat ttcgtataaa tgaaacgtgt cttcggataa agccagttga gaaagccatc
      841 cggattgaaa gccggaaacg taatcatgga tagatcggag aatctgcttt ttgccttcag
      901 atgctggcgt cgctcctctt tgctttgtaa aaggagcagc acattctttt cttgcggcgt
      961 ctggtaccag aagccggccg tttgcagttc cgaagcaaac tcgaatatcg tttgctcgtc
     1021 gtagaacgtg cccatttgtt gggaatagag ctcggcaatc gtggcatagg aacggttgcc
     1081 gtcgaacaac cgcgccagac tccattgggc ccacgttatg cggtacatca ggccggtgct
     1141 cgggaaatat actcggacga tccgctcgcc atcttcgaca tgttcacgga aagtcgcgct
     1201 gggttcgatg cgggggacac gttgcagaat ttctcgcgcc ggaatgtccg gcagggccac
     1261 atcgagcgcg cgcgtcaggt tcattgggta tctgggacgt gtacagtgac ggtcatgccg
     1321 gggcgaagct ctccgcactc gccgaccaat tctgcaagga cttcgatggt cccgctggaa
     1381 gggtctacca ccggactgac ttccacaatt ttgacgtcat gcttttccgc gacgaggtcg
     1441 gaacgggtca gtggcaaatg ctggcctttc ttcacgcggc ggatgaattt ttctgggagc
     1501 gtgaagcgaa cgcgcagcgg tccttccgcg gtgacccaga agagccgatc ggctttgcct
     1561 acggattggc cctcgcgaac gtagcgtcgc gccaccaagc cggcaaaggg cgccatgatg
     1621 cgcgtttttt ccagttcgag ttccagcgag tgttcttctt ctttggcagt attcagctgt
     1681 tccttgacgc ggaggatgtc ccattggtcg gattcggctt tgtactttgc gtgctgcaac
     1741 tgttcttccg cgatcagctt ctccgcgtag agtcgctgcg cgcggacgta gtcggctttg
     1801 agaacctcgg cctcggcctg ccagtttttc aggtcggcgg cgatgcttcg cgtcttggcg
     1861 cgcgccgctt ccagattggc ggtcagctgg cgattgtcca gctgtgccaa aagcgtgccg
     1921 gctgcgaccc gagtgccggt atccgccgaa attttcgcga cgataccttc gcgctgtgca
     1981 gtcacatcca cctgatgttc gacaatcaag ggtgcggtaa tcgtgagcac attccctgaa
     2041 gcatcgcccg aattgtgtgt aggggccgtc gcagccgtcg ctgagttttt ctccggagcg
     2101 gacgccgcct gtgccgcgga gggactttct ttctggtcac cgcaaccgga gagcgctgcg
     2161 acaaacatcg tggcgagaag cgctttgcac cgaggcttca tagtgttggt cctcaagttt
     2221 ctcaccagcc aaaccaattc cagatgcgcg agtaaagcca gatcgccggg cggcgaaaca
     2281 gaacatagcc cgacatgcgc catccggtgg aaatcttggc gcggccctcc atgccggtgc
     2341 gaatcaatcc ggatgcgttg tcgactgcca cgcgtgcaaa gaacacgcgg gagtctcctt
     2401 gcgcctcgcc tttgggactc accaccgtga ccgttccgtg aaacgtgcga gtaggatagc
     2461 cgttgagctt gatggcggcc ctctgccccg cgcgcagcaa gctgacgtcg tagtcgtcaa
     2521 tcgccacatc caggatggcg gttgaagcgt cgaccacgtc ggcaaaggcg tcgccaaact
     2581 gcaggcgtct cccaacaaac gtgtcaatgt gcggcgtggt gaccaggcca tcgatcggag
     2641 agcgaagttc cgcacgatcc agcaactgct gcgcgttggt tacttccgtc ttccagtaat
     2701 ccgcctgaac gcgctgaatc cctgcctcag cgtcgtcgtt cgaagccaag gcgtggttca
     2761 tctgcagcaa agcagtttga taacgcgcct gcgctgctgc cagcgccgtt tgtctgtccc
     2821 aggattccat ttcggcaatc gcttgccctc gtttgaccgg ttcgccctcg tggacaaaaa
     2881 cctttccaat tacgccttcg aactctggct gcacttgcgc gctgcggacc ggcgcgacga
     2941 ccgcgtctcc cgccaaccgc atgggcagcg ggaaaatcac cagaaagaca agcgcggcca
     3001 ttgccgcaac agtgtagagg gtgcgccgcg tcttctccat cgccaggaag cgccgttttc
     3061 gctccaacac cggctccagc acggaaatga acggcacttc tttgtacatt tgcgcattgc
     3121 gcagcgccac cgtcgcctgc ccggccagga cttgcagcag ctcgaggtgc acggaggtca
     3181 gaaaatcggg atcgctgctt tccaggctga gaatgccaac ccgaccggta tcgtcgttca
     3241 gggggatggc gtaaaagccg cgcatgccgg tttcagcgaa atattgccgg aacttggcgc
     3301 gcgtttcttc gcggtcggtg tcaatctgct cgtcatgctg acgtacgttg acgatttcct
     3361 ccgctagcgc cgcccattgc agcacgccgt tgagaggagc gagatccggc gcgtcgggat
     3421 tcacttgcgt cacgcccgta acggcgctca gcttgaagcg gccgcgctgc tgcagcgcaa
     3481 ttgccgcgcg ttcataggga atcacggcct gtggagcgtt tacgatggtt tgcagcatgc
     3541 gctccagatt cagcgtggaa gtaatttcgt ggcttaccgt gaccagcgtt tccagcactt
     3601 ctactttgcg ttcagccatc agcaggctgg cgttgtggag cgcgctcgac gcggtttcgg
     3661 tgatcgtggt gagtgcgaaa aggtcatcgt cgtcgaaagg cgctccggtc agtttgttta
     3721 tcgcctccac gacgcctacc agtgcgccct gatccagaat cggaaccacc agtagcgtcc
     3781 taacgccacc atcgccatgc accgtcgcgt tgcgcgtcgt caaccgtggg tcatccgcgt
     3841 tggcgatgag cacggcttcg ccgtcatcgg agacttggcc ggcaattcct tcgccgggtc
     3901 tttgcacgct gcctgcgggt gtagtggcat cgctgccgga ttgctgcatc agctcgatgc
     3961 tttcgtcccc ccgcaacaac cacaaattga tggccgcgca ttccatcact tcgtggaatt
     4021 tcgagccgat gatgggcagc agctcgtcca tttccagggt cgaggaaaac gaacgctcca
     4081 ggtcatagag ctgcgtcagt ctggtcaccg aggcgagtga gtcgttgcgc tccgcttcgt
     4141 atttttgcgc agcggccagc gctgtcgcgc tgatttgcgc gagcggctgc aacgcttgca
     4201 actgttcggg tatcagaacc gagtcaaacg acagaatttc gattgcgccc gtcaaggcag
     4261 cgccctgtgc taacggcaga taggccagcg agcggagcgt gcggcgaaca tcgaggtgcg
     4321 cgtaatcctc gcgagacaaa tccgcgcccg aaaaaagcag ggctgtctgc cgcgcttgca
     4381 gttcgccgag gctgccttcg ccaatcggtg catgcccggc catgacggat tcgccccctg
     4441 cggacgcacg caccacccac gcttcccggc catcgatcgt tccgattgtg tagatattga
     4501 cgaacgcgcc cgtaacggct tcggcgaccg cttgcgcaat gcggcgagcc cgcggcgcca
     4561 attcctgcgc cgcaagcagc tgagctgcca gaaaatttag atcaaatttc aatcagggtc
     4621 tctgctgacc agcacacgcc gacccgagat tgcccctggt cgcggtggct ggtcaccgcc
     4681 gacttgcaaa tctgccggaa tccttctaag ttgagcgcga actttagcag accgtacacg
     4741 cccaggcaag catcccgcag ttcgcaacgg gccgtgtaaa ccggggagcc gatcgtggaa
     4801 attgatgtgc gcacgcagga tcaggtcaag gtcatcaagc tgcgcggcaa gctgcatctg
     4861 gggccggcgc tcgaccgcac caacgacacc atcaaggatc tgctgaactc cggtgaagcg
     4921 cggctggtgg tggatcttga ggaagttccc atgattgatt cgagcggaat cggtctgctg
     4981 gtgcggcatt tgactatggc caagcagcgc ggcggcgcct tgaagctgct gaagccctca
     5041 aaatttgcgc tgcagaccct caaagtggta cgggtgttga atttatttga agtgttcgag
     5101 gaccagcagt tagcggttgc gtcgtttcag aagtgagtcg gtgttcgctg gctctgggct
     5161 actcgctgcc caggaatgca taccggaaga ggaacagcac ggaaagtatc cagataagtg
     5221 ggctgatttc cttgtgcctg ccggcgccta gtttcaaaaa tgtgaaagag agtaagccga
     5281 ggctcagtcc ggtggcgatg ctgaacgtta acggcgtcag aatcaccgcc aggaacgctg
     5341 gaaacgcttc cgtaaagtca tcccacttta cgcgtgagat tgctccgcac atcaacgcgc
     5401 cgacgagaat cagagccggt gcggtggcgt atgccggtac agctgcgacc agcggcgcgc
     5461 aaaacatcgc aacgccaaaa aacgctgcga tcagcaagtt acccagcccc gttctgccgc
     5521 ccgccgcgac tcctgctgcg ctctcgatgt agctcgttac cgtcgacgtt cccgtcaatg
     5581 cccccgccat g
//
LOCUS       DASTPM010000652         4401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_340819, whole genome shotgun sequence.
ACCESSION   DASTPM010000652 DASTPM010000000
VERSION     DASTPM010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340819"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFR42_11470"
     CDS             <1..599
                     /locus_tag="VFR42_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369825.1"
                     /translation="PGSLGASYFRFRGRPIRPRPVLPIVPFPVFFGGPFFGYWPGYGL
                     NSLWWPSCAPFWGWRFGCTAVPYAPYYRYDFGGYISPYVSPYSAPPEYAPPPSYLYGG
                     EESRQRPQLYLKDGTVYNVTDYWLVNGQIHFTTLEEDGTKPVEHVIPFEDLDLQQTID
                     MNTRMGFRFVLRNAPIEHYLQNRNEGNPPAEKVPPQEN"
     gene            1162..3843
                     /locus_tag="VFR42_11475"
     CDS             1162..3843
                     /locus_tag="VFR42_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEU0369826.1"
                     /translation="MLRRIAIVAALTAVRTFLITAPTEAAEAAGKAVTSLELLKGWEI
                     QSSCDIKAGGEQVSRAGFATAGWHKTTVPNTVVGALVDDKTYPDPTYGTNMKNLPGMN
                     YSTATFFANQDMPDGSPFKCAWWWRNEFVAPASFAKKNVSIHFPGINYRANVWFNGQK
                     IGDSKEIAGTYRSFEFDLTKLVKVGAKNVIALEISAPAKEDLGITWVDWNPTPADKNL
                     GIWKEVTLASRGPVAIRNPFVKTHLNADFTAADLTISADLRNDSGAATKGILVAEVDG
                     KMLKLPVELAAGESKSVRFEPEHFAALKLDRPKLWWPYTIGAPYLYKAKLHFESGAEV
                     SDTTTVTFGVRQVTSELTNQGHRLFKINGRRILIRGAAWAPDMFLRPMPRKLDDDLRY
                     VKHMGLNTIRLEGRIDRDELFNKTDEMGILVMPGWTCCDMWEQWKDWTDETHKIAAAS
                     MADQARRLRNHASVFVWLYGSDGPPPADVEKMYLSVLAEAEWPNPSVSSASETPTTVT
                     GKSGVKMTGPYEYVPPVYWLADTQAGGAYGYNTETSPGPAIPTLESVKRFIPKEHLWP
                     IDDVWNYHAGGERFTTVNVFNDGLNRRYGPATSLEDYERKAQAAAYDGERAMFEAYGR
                     NKYTSTGVIQWMLNNAWPSLIWHLYDYYLVPGGGYFGTKKAMEPLHVQYDYDDQSVAV
                     VNDTYVERNSMKVCARVYSLEGKLLADKEESVDVPADGAVKAFDLPKPEGLTTTFFVR
                     LDLRDARGRAVSDNFYWLSAKADTLDWAKKQDTVYTPQAEFGDLTGLAKLPQVRLEAS
                     AAIEQAANSGAEKAHIRVKNPSTGVAFQVRLRLANKKDDFDVVPVFWDDNYFSLLPGE
                     ERLVSVSYDASQHGEHPVIQIGGFNIAGGEIVAGGPH"
     gene            3895..>4401
                     /locus_tag="VFR42_11480"
     CDS             3895..>4401
                     /locus_tag="VFR42_11480"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0369827.1"
                     /translation="MKIHSRHARRIHSADAATLFVVSLLSCAAIFAQDAKLAAAQVAI
                     QKRITASGANVAVYFKTLDGKAEWSVRPDDVFHAASTMKIPVLIELFHQVQQGKVKLD
                     DKIVLKNEFHSIADGSPYQLSAADDSEADVYKAEGQARTLGELCELMITVSSNFATNL
                     IIEKLGVEN"
BASE COUNT          989 a         1267 c         1205 g          940 t
ORIGIN      
        1 atcctggcag ccttggtgcg tcctatttcc gctttcgagg ccgcccgatt cgcccgcgcc
       61 cggtgctccc aattgttccg tttcctgtct ttttcggcgg gccctttttt ggctattggc
      121 cgggatatgg actcaactcc ctctggtggc caagctgcgc tcccttctgg ggctggaggt
      181 tcggctgcac tgccgttccc tatgctccct attatcgtta cgacttcgga ggctacattt
      241 ccccgtatgt ctcgccgtac agcgctccgc cggaatacgc gcctcctcct tcgtacctgt
      301 acggcggaga ggagagccgg caaaggcccc agctttattt gaaagacggc acggtctata
      361 acgtcaccga ttactggctg gtgaacggcc aaatccattt cacaaccctc gaagaggacg
      421 gaaccaaacc ggtcgagcac gtcattccct tcgaagacct tgatttgcag caaacgattg
      481 atatgaacac gcggatgggc ttccgctttg ttctacgcaa cgcgcccatc gagcactatc
      541 tgcaaaatcg taacgagggc aatccgccgg ccgagaaagt tcctccgcaa gaaaactaac
      601 ggcaccgcaa agattgctca tccctggttc gtactatccc gacgatcacc gaaagtccta
      661 tgtaaacaag taacatctct gagtagcctg gaatagcgat gaagacagtt gctgtttttt
      721 actgccgctg gttgattgtt tggctatttg agttccgaaa gtatcctctt gaacttctcc
      781 ggcaagatta tgtcctgcga tcgctgctcg tgatctttca ctgtccagcc gaaaatccgg
      841 cttgtcgtgt acgttgatcg ttactttctg gaagtcgata tccgagtatt tatctttctc
      901 gtcaacggcc ggcctcctcg aagaagggca gcctggcacg ctggatacaa taccggcaac
      961 aattagaatc atgtttcgaa aatggcgcat aaaccaaggc tagaatgtga tatggcagcg
     1021 actccttcct ccggaaccaa acatctagcg ttacgtccga tattttgttt ttcgcgacga
     1081 cactccggca caaaacctgt tttcttgctg tttgaatcgt tttaatatga ctccgccttg
     1141 attcacgtga aatgaggact catgctccgt cgcatcgcga tcgtcgccgc cctgactgcc
     1201 gtccgtacct tcctgatcac tgcgccaacc gaggcggccg aagctgccgg gaaggccgtc
     1261 acatccctcg aactcctgaa aggctgggag attcagtcgt cgtgcgacat aaaagccggc
     1321 ggcgaacagg tttcgcgcgc gggttttgcg acagctggct ggcacaagac gacagttccc
     1381 aataccgtag tcggtgcgct ggtggacgac aagacttacc ccgatcccac ctacgggacc
     1441 aacatgaaga atctgccggg gatgaactat tcgaccgcca cattctttgc gaaccaggac
     1501 atgccggatg gcagcccgtt caagtgtgca tggtggtggc gcaacgagtt tgttgcgcca
     1561 gcatcatttg cgaaaaagaa cgtgtccatc cactttcccg gcatcaacta ccgcgcaaat
     1621 gtctggttca acggacagaa aattggcgac tctaaagaaa tcgccgggac ctaccgcagc
     1681 tttgaattcg atttgacaaa gctcgtgaag gtcggtgcaa aaaatgtgat cgccctggaa
     1741 atcagcgcgc ccgccaaaga ggacctgggc atcacctggg tcgactggaa cccgactcca
     1801 gcggacaaga acctggggat ctggaaggaa gtgacccttg cttcgcgtgg tccggtggcg
     1861 attcgcaacc cgtttgtgaa gacgcatctc aacgcggatt ttaccgccgc ggacctgacg
     1921 atctccgccg atctgcgcaa tgactccggc gccgccacga aaggaatttt ggtagccgaa
     1981 gtcgacggca aaatgctgaa gttgccagtg gaactcgctg ccggagaaag caagtcggtc
     2041 cgcttcgaac cggaacattt tgccgcttta aaattggacc ggcccaaact gtggtggccg
     2101 tacaccatcg gcgcgccata tctctataag gccaaattgc atttcgagtc tggcgccgag
     2161 gtttccgata ccacaaccgt gacattcggc gttcgccaag tcaccagcga actgaccaac
     2221 caaggccatc ggctcttcaa gatcaacggc aggcgcattc tgatccgcgg cgcggcgtgg
     2281 gcgccagaca tgttcctgcg gccgatgccg aggaaactcg acgacgacct gcgctacgtg
     2341 aagcacatgg gactaaatac gattcgcctg gaaggccgga tcgatcgcga cgagttgttc
     2401 aacaagaccg acgagatggg cattctcgtg atgcccggct ggacctgctg cgatatgtgg
     2461 gagcagtgga aagactggac ggacgagact cacaagatcg cagccgcttc gatggccgac
     2521 caggcgcggc gcctgcgcaa tcatgccagc gtttttgttt ggctgtatgg cagtgacggg
     2581 cctccgcccg ccgacgtgga aaaaatgtat ttgtccgtgc ttgccgaagc cgaatggccc
     2641 aacccttccg tttcgtccgc cagcgaaacg cctaccactg tcacgggaaa gtccggtgtg
     2701 aagatgacag gtccctacga atacgttcca ccagtgtatt ggttggccga cacgcaggct
     2761 ggcggcgctt acggctataa caccgagacc agtcccggcc cggcaattcc cacgctcgaa
     2821 agcgtgaagc gcttcattcc gaaagaacat ctctggccca tcgatgacgt gtggaactac
     2881 cacgccggcg gcgagcgttt taccaccgtg aatgttttta acgatggact gaatcgtcgc
     2941 tacggaccgg cgacttcgct cgaagactac gaacgcaaag cgcaggccgc ggcttacgac
     3001 ggcgagcgcg ccatgttcga ggcctatggc cgcaataaat acacttcgac cggcgtcatt
     3061 cagtggatgc tcaacaacgc gtggccatcc ctgatctggc atctttacga ctattaccta
     3121 gttcccggcg gcggctattt cggcacgaaa aaggccatgg agccgctgca tgttcagtat
     3181 gactacgacg atcagagtgt agcggtggtg aacgacactt acgtcgaacg taatagcatg
     3241 aaagtgtgcg cgcgagtcta ctcgctcgaa gggaagctgc ttgccgacaa ggaagagtcc
     3301 gtggacgttc cagccgatgg cgcggtaaag gcgttcgatt tgccgaagcc agaagggctc
     3361 accacgacgt tttttgtgcg gctcgatttg cgcgacgcgc gcggccgagc agtgagcgac
     3421 aacttttatt ggctttcggc caaggcggac acgctggact gggctaagaa gcaggatacg
     3481 gtgtacacac ctcaggccga atttggcgat ttgaccggtc tcgcaaaatt gccgcaggtt
     3541 cgtctcgaag ccagcgcggc gattgaacaa gccgccaact cgggtgccga aaaagcccac
     3601 atccgcgtca aaaatccttc cacgggcgtg gcgtttcaag ttcgcttgcg gctggcaaac
     3661 aagaaagacg atttcgacgt ggtgcctgtc ttctgggacg acaattattt ctcgctgctg
     3721 cccggcgaag agcggctcgt ctccgtcagt tacgacgcct cgcaacacgg tgaacatccg
     3781 gtcattcaga ttggcgggtt taacatcgcc gggggagaga tcgttgctgg cggcccacac
     3841 taggcaaacg cgctggattg ctttgccgga cggcttcgtg caaaattccg ctcgatgaaa
     3901 atccacagcc ggcatgcgcg gcgaattcac tccgcagacg ccgcaacgct ctttgttgtt
     3961 tcgctcctta gctgtgcggc tattttcgct caggatgcca aacttgccgc cgcccaggtg
     4021 gccatccaaa aacgaatcac tgcgtcgggc gccaacgttg ccgtgtactt taaaacgctc
     4081 gacggcaaag ctgaatggtc ggtgcggccc gacgacgtct tccacgccgc cagcaccatg
     4141 aaaataccgg tcctgatcga gctgtttcac caggtccagc agggaaaggt gaagctcgac
     4201 gacaaaattg tgctcaagaa cgagtttcac agcatcgcag acggctcgcc ctaccagctc
     4261 agtgccgccg acgattccga ggcagacgtg tacaaagccg aagggcaggc ccgcacgctg
     4321 ggcgaactgt gcgaattgat gattaccgtc agcagcaatt tcgccacaaa tctcattatc
     4381 gagaagctgg gagtggagaa c
//
LOCUS       DASTPM010000653         7272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1147843, whole genome shotgun sequence.
ACCESSION   DASTPM010000653 DASTPM010000000
VERSION     DASTPM010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147843"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFR42_11485"
     CDS             <1..616
                     /locus_tag="VFR42_11485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369828.1"
                     /translation="ISRGGRIARTSFDEAELMAQRAIERKRDCDGSWNILGRALMSQG
                     KYEQGAKLAVDAIEANGDDYNTYIPFGVCLENIGRMAEGTKMREQMNVVLRKQLETVP
                     EDVRARILLASNLANAGEADEAIRHLQTAVALRPNDGNTLYNAACTYGCLKMKTESLD
                     TLKRAIAAGYGNLNWAARDPDLNCLHDNAEFRGIVGMPGASAAS"
     gene            681..3086
                     /locus_tag="VFR42_11490"
     CDS             681..3086
                     /locus_tag="VFR42_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0369829.1"
                     /translation="MINETILHYRILEKLGGGGMGVVYKAEDTRLDRFVALKFLPPDL
                     AQDAHALERFRREAKAASALNHPNVCTIYDIGEHDSRAFIVMEYLDGATLKQRIGGRA
                     LDIETLLATGIEIADGLAAAHAKGIVHRDIKPANVFVTALGHAKILDFGLAKVAEQAG
                     SSASENTLTRTVDAEHLTSPGSTLGTVAYMSPEQAKAKELDARSDLFSFGTVLYEMAT
                     GTLAFRGESTALIFKAILDESPVSPLRLNPELPAGFEPIVYKALEKDRSLRYQSAADL
                     RSDLARLKRDLESGRAPSSAVSAGVAGAAPSSPTVAPASAASSISAPSSSRKYLVGGL
                     AAVVLLAAAAGIFWMRGKTDSPKVTSIAVLPFVNATADPNNEYLSDGLTESLIGTMSQ
                     LPNLKVMARSTVFRFKGNQDDPQKIGQTLQVGAVLMGRVTQRGDELSVQADLVNTADG
                     TELWGSRYSRKLADITQVQGDITRDISNRLQMHLSDTAQQKLGRAGTINPEAYRLYLE
                     GRQQWYGRTPEGIKKSVELFQRAIVADPNYALAYAGLADTYNVATGYGTVIQPKQAFA
                     LADQASRKALELDDTLSEAHNARAITLTNQLKWSEAEGEFRRAIALNPNDSSAHYFYA
                     FLYLMPQNRIEESLQEFRTALSLDPLSGIMNVNYALTLMVARRSPEAISHMQKLLERD
                     PSFSPAHFYLSQIYVSNGRYAEAVSELRKTTFGRVKGPASPDLEGYLKLMLTAGDTAP
                     PTNIAVTYALAGDRNKAFEYLEKAYTEQDSELGACIRFPAFDVLKSDPRWAAYMRRLG
                     LPE"
     gene            complement(3224..3517)
                     /locus_tag="VFR42_11495"
     CDS             complement(3224..3517)
                     /locus_tag="VFR42_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369830.1"
                     /translation="MIQEAVANNASSPVSLPLQRPELAALRSSAAAIESTPGCRSFEL
                     VWSRYAAYLVTEEGVGSCGSYDHEDFTGGLFRIYSKSHFLAISHAIAAGTSSL"
     gene            complement(3615..4556)
                     /gene="mdh"
                     /locus_tag="VFR42_11500"
     CDS             complement(3615..4556)
                     /gene="mdh"
                     /locus_tag="VFR42_11500"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU0369831.1"
                     /translation="MSRKKVTVVGSGFVGSTTAQRIVDLELADVILTDILDGPPAGKA
                     LDMVEATPITKADVHAVGISTASGDYSATAGSDIVVITAGFPRKPGMSRDDLLKANYD
                     VVKAVVEQIVKHSPNCIIIVVTNPLDAMAQTAFRVSSFPKQRVLGMAGVLDSARMSAF
                     VAMECRVSVENVHSFVLGGHGDDMVPLPRYSSVAGIPLPDLLSKDRIDAIVERTRKGG
                     AEIVNLLKTGSAYYAPSAAAVEMVEAILKDKKKILPCAVYLEGEYGIKGLFVGVPVKL
                     GAKGVEQIVEIKLTVEENAALQKSSASVRELVTVLGL"
     gene            complement(4585..4908)
                     /locus_tag="VFR42_11505"
     CDS             complement(4585..4908)
                     /locus_tag="VFR42_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369832.1"
                     /translation="MPMSQPWIGLLELRKLPGCEIMGTPGAFVNVITVAIPDRESQDK
                     VLGDVKKACSFLNEIEDLEPFAERVSKYEVDDELDGALSESREPYRRSSFWYFSCLFA
                     GRGVS"
     gene            complement(4919..6340)
                     /locus_tag="VFR42_11510"
     CDS             complement(4919..6340)
                     /locus_tag="VFR42_11510"
                     /inference="COORDINATES: protein motif:HMM:NF005610.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU0369833.1"
                     /translation="MKSSYNGQAVPSDGTPIEYAGGELQVPANPIIPFIEGDGTGRDI
                     WKASRRVFDAAIEHAYGGKRRIAWYEVFAGEKAFNTFKEWLPNDTVEAIRDFRVSIKG
                     PLTTPVGGGIRSLNVALRQILDLYSCERPVRYFEGVPSPVRHPEKLNVFIFRENTEDV
                     YIGIEWKSGSPEAKKLLEFLNNDMLKDGKKQIRWDSGVGIKPISPTGTKRLVRRAIKY
                     ALAHQRKSVTLVHKGNIQKFTEGGFRDWGYQLAQEEFRAQTITERESWIIDNLDKNPQ
                     LSIEQNAAMIEPGLEQATDAFQKTIYDEVRHTLAAIYKTHGQGQWRQKLMINDRIADS
                     VFQQVLMRPDEYSVLATSNLNGDYLSDACAAQVGGLGMAPGSNIGDGFGVFEATHGTA
                     PKYADKDVINPGSVILSGAMMLEFLGWNEAAKLIEDGIARTIQQKKVTYDLERLMPGA
                     TKVGTSAFATAIIENMKGLAAAG"
     gene            6610..6963
                     /locus_tag="VFR42_11515"
     CDS             6610..6963
                     /locus_tag="VFR42_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0369834.1"
                     /translation="MMLDWNEYQKQLNGRIAEIAKLSTETVKGYAMLSSANAKTTHLG
                     EKVRQLICLAAAVTLRCDGCIVFHTDAALKAGANKEEISEALGVAVAMNAGASMVYSA
                     RVMDAVEAKTSRAAA"
     gene            complement(7012..>7272)
                     /locus_tag="VFR42_11520"
     CDS             complement(7012..>7272)
                     /locus_tag="VFR42_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU0369835.1"
                     /translation="KVDFLCRDSILAAPIVLDLVIFLDLAQRAGFRGIQEWLSFYFKS
                     PMTGPDLYPEHDLFIQSMKLKNTLRWMMGEDLITHLGQEYYD"
BASE COUNT         1542 a         2049 c         2179 g         1502 t
ORIGIN      
        1 gataagccga ggcggaagga tcgccaggac ctcgttcgac gaagcggaac tgatggcgca
       61 gcgtgcgatc gaacgaaaac gtgactgcga tggctcgtgg aacattctgg gccgggcgct
      121 gatgtcgcaa gggaagtacg agcagggcgc aaagctggcg gtggacgcga ttgaagccaa
      181 cggcgacgat tacaacacct acatcccgtt tggcgtatgc ctggaaaaca ttgggcgaat
      241 ggcggaaggc accaagatgc gcgagcagat gaatgtggta ttgcgcaagc agttggaaac
      301 ggtgccggaa gatgtgcgcg cgcggatttt gctggccagc aatctggcca acgccggaga
      361 agctgatgaa gccatccggc atttgcagac agcggtggcg ctacggccta atgatggcaa
      421 tacgctctac aacgcggcgt gtacgtacgg ctgtttgaag atgaagacgg agtcgctgga
      481 cactttgaaa cgcgcgattg cggcgggata cggcaacttg aactgggcgg cacgcgatcc
      541 ggacctgaac tgcctgcacg acaacgccga gttccgaggc atagtcggaa tgcccggggc
      601 cagcgccgct tcgtgaagaa cgctgggcag gaaagccgtt tgactcactc tcggcgaggc
      661 acctaccata acggcaccgc atgattaacg aaacgattct gcattaccgc atcctggaaa
      721 agttgggtgg cggcggtatg ggtgtggttt acaaggcgga agacacccgc ctggaccgct
      781 tcgtggcgtt gaaattcctg ccgcccgatc tggcgcagga tgcgcacgca ctggaacgtt
      841 ttcgtcgaga agctaaagct gcgtcggcgc tgaatcatcc gaacgtttgc acgatttacg
      901 acattggcga acacgatagc cgcgcattca tcgtgatgga gtacctggac ggcgcaacgc
      961 tgaagcagcg gatcggcggc cgagcgctgg acatcgaaac gcttctcgcg acggggattg
     1021 agattgcgga cgggctggcc gcggcgcacg cgaagggaat cgttcatcga gatatcaagc
     1081 cggcgaatgt tttcgtcacc gcactcggcc acgccaagat tttggatttt gggttggcca
     1141 aggtggcgga gcaggcgggc tcgtcggctt cggaaaatac gctgacgcgc acggtggatg
     1201 cggagcactt gaccagtccc ggctcgacgc tgggaacggt ggcgtacatg tcgccggagc
     1261 aggcgaaggc gaaagagttg gatgcgcgca gcgatctgtt ctccttcgga accgtgctct
     1321 acgaaatggc cacagggacg ttggcgtttc gcggtgagag tacggcgctt attttcaagg
     1381 cgattctgga tgagtcgccg gtgtcgccgc ttcgtctgaa tcccgaattg cccgcgggct
     1441 tcgaaccgat tgtctacaag gcgctggaga aagatcgcag tctccgctat cagagcgctg
     1501 ccgacttgcg cagcgatctg gccagattga agcgcgattt ggaatcgggc cgcgcgccca
     1561 gctcggcggt ttccgccggt gttgcgggcg ctgctccaag ttcccccacc gtcgcaccgg
     1621 ccagcgcagc ttcatccatt tccgctcctt cctcttcgcg aaagtatctg gttggcgggt
     1681 tggcagcggt tgtcttgctg gcagcggcgg cggggatttt ctggatgcgt ggcaagacgg
     1741 actcgcccaa agttacttcc atcgcggtgc tgccgtttgt gaacgccacc gccgatccca
     1801 acaacgaata cttaagcgat ggcttgacgg aaagcctgat cggaacaatg tcacagttgc
     1861 cgaatttgaa ggtgatggcg cgcagcacgg tgtttcgttt taagggcaac caggatgatc
     1921 cgcaaaaaat cggccagaca ctgcaggtgg gcgcagtgct gatggggcgc gtgacgcagc
     1981 gcggcgacga gttgagcgtg caagccgatc ttgtgaatac ggcggacggc acggagctgt
     2041 ggggttcgcg ctattcgcgc aaattggcgg acattacgca ggtgcagggc gacatcacgc
     2101 gcgacatttc gaaccggctg cagatgcatc tgagcgatac cgcccaacaa aaattgggcc
     2161 gcgccggaac catcaatccc gaagcgtacc gcctctatct ggaagggcgg caacagtggt
     2221 atgggcgcac gcccgaggga ataaagaaaa gtgtcgagtt gtttcagcgc gcgattgtcg
     2281 ccgaccccaa ttacgcgctg gcctacgccg gcttggcgga tacctacaac gttgcgaccg
     2341 gctacggcac ggtgatccag ccgaagcagg cattcgcgct ggcagaccag gcatcgcgga
     2401 aggccctgga gctggacgat accttgtccg aagctcataa cgcgcgggcc atcaccctga
     2461 ccaatcagtt gaagtggagc gaagccgaag gcgagtttcg ccgcgcgatc gcgctcaacc
     2521 ccaacgattc ctcggcgcac tacttttacg cgtttttgta cctgatgccg cagaatcgga
     2581 ttgaagagtc tttgcaggag ttccgcacgg cgctctcgct ggatcctttg tcgggaatca
     2641 tgaatgtgaa ctatgcgctg acactcatgg tggcgcgccg ctcaccggaa gcgatttcgc
     2701 acatgcagaa attgctggaa cgcgatccgt cgttcagccc ggcccatttc tacttgtcgc
     2761 agatttatgt ttcgaacggg cgctacgcgg aggcagtcag cgaactgcga aagacgacct
     2821 tcggtcgggt gaagggcccc gcgagtccgg acctggaagg gtatttgaag ctgatgctga
     2881 cggcaggcga cacggcgccg cccaccaaca ttgccgtcac gtacgcgctt gccggagacc
     2941 gcaacaaggc gtttgaatac ctggagaagg cctacaccga acaggactct gagttaggcg
     3001 cgtgcatccg gttcccggct ttcgatgtgc ttaagtccga tccgcgctgg gccgcctaca
     3061 tgcgccgcct cggcctgccg gagtagcccc ataaaacggg agtcgcgcga aagcctattg
     3121 gattcttttg gctctgatgc gtccgggtcg atttcctgaa tttccggcgg agcatatgaa
     3181 gcgacatcga ttagatggtt gaggcaaaca atcttgtagt gcatcagagg ctcgaagtgc
     3241 ccgccgctat cgcgtgagag atggcgagaa aatgggactt cgagtaaatg cggaataacc
     3301 cgccggtgaa gtcctcatgg tcgtagctgc cgcacgaacc gacgccctcc tcggtcacta
     3361 agtaggcggc ataccggctc cataccaact caaaactgcg gcagcccggc gttgactcga
     3421 tcgctgcggc gcttgacctt agcgcggcca actcaggacg ctgcaaagga agtgatacgg
     3481 gagaggacgc attgttcgca acggcctctt gaatcatgat cctgagcgag ttgtcgcgag
     3541 gctaagagag ttcctgaagg tagatgtact tgctctgaca ggaattcgca ggtcagaccg
     3601 ccttgcataa aaccctaaag ccccagcacg gtgacaagtt cgcggacgga ggcggatgat
     3661 ttctgcaaag cggcgttttc ttcgacggtt agcttgattt cgacgatttg ctcgaccccc
     3721 ttggcgccga gtttcaccgg cacgcctacg aagagtccct tgatgccgta ctcgccttcc
     3781 agataaaccg cgcagggaag aattttcttt ttgtccttga ggatggcttc gaccatttcc
     3841 acggctgcgg ctgacggggc gtagtaggcc gagccggtct tgaggagatt gacgatttcc
     3901 gcgccgccct tgcgggtgcg ctccacgatg gcgtcgatac gatctttcga cagcaaatcg
     3961 ggaaggggaa ttccggccac gctggagtag cgcggcaatg gaaccatgtc gtcgccatgg
     4021 ccaccgagaa caaacgaatg cacattttcc acgctgactc tgcattccat cgccacgaac
     4081 gcgctcatac gcgccgagtc cagcactccg gccatgccca acacgcgctg cttggggaag
     4141 ctgctgactc taaacgcggt ctgtgccatg gcgtcgaggg ggttggtgac gacgatgatg
     4201 atgcagttgg gcgagtgctt gacgatttgt tcgacgacgg ctttgaccac atcgtagttc
     4261 gctttgagaa ggtcgtcacg gctcatgcca ggcttgcgcg ggaacccggc agtgatgacg
     4321 acgatgtcgc tgcccgcggt ggcagagtag tctcctgatg cggtggagat gccgacggca
     4381 tgcacgtcag ccttggtaat cggcgtggct tcgaccatat cgagcgcttt gcccgcgggc
     4441 gggccgtcca ggatgtcggt gaggatgacg tcggcaagct cgagatcaac gatgcgctgc
     4501 gcggtggtgg atccgacgaa gccggagccg accacggtga ctttttttct gctcatgcga
     4561 ttttctcccc atcaggaaag actcttaact tacgccccgt cccgcgaata ggcatgaaaa
     4621 gtaccaaaac gaacttcgtc ggtagggctc ccggctctcg ctgagagctc catcaagttc
     4681 atcgtcgact tcatactttg aaacgcgttc ggcaaacggc tccaagtctt cgatctcgtt
     4741 caggaaagag caggctttct tcacatcgcc gagaactttg tcttgagact cccgatcggg
     4801 aattgcgacc gtgatgacgt taacaaatgc accgggcgtc cccatgattt cgcatcctgg
     4861 cagcttgcgg agttccagca aaccgatcca tggttgcgac atcggcaagg aagaagtcct
     4921 agccggcggc ggccaggccc ttcatgtttt cgataatggc ggtagcgaag gccgacgtcc
     4981 cgactttcgt cgcgccaggc atcaggcgct ccagatcgta agtgactttc ttctgctgga
     5041 tggtgcgggc aatgccgtct tcgatcaact tcgccgcctc gttccagccc agaaactcca
     5101 gcatcatcgc tccggaaaga atgaccgagc cggggttgat cacgtccttg tccgcatact
     5161 tcggcgccgt gccgtgcgtg gcttcaaaaa cgccgaaacc gtcgccgata ttcgacccgg
     5221 gagccatgcc gagtcccccc acctgcgccg cgcaggcatc ggacaagtaa tcgccattca
     5281 ggttcgaagt cgccagcacg ctatattcgt ccggccgcat cagcacttgc tggaaaacgg
     5341 agtcggcgat gcggtcattg atcatcaatt tctgtctcca ttgaccctgg ccgtgcgtct
     5401 tgtaaatcgc agcgagcgta tggcgcactt cgtcataaat agtcttctga aaggcgtcgg
     5461 tagcctgttc gagtcccggc tcgatcatcg cggcgttttg ttcgatgcta agctgcggat
     5521 tcttgtcgag gttgtcaatg atccagcttt cgcgctcggt gatggtctgc gcgcgaaatt
     5581 cttcctgcgc cagctggtaa ccccagtcgc ggaatccgcc ctccgtgaat ttctgaatat
     5641 ttcctttgtg taccagcgtg accgacttgc gctggtgcgc cagcgcgtac ttgatcgcgc
     5701 ggcgcaccag ccgttttgtt ccggtcggcg aaatcggttt gatgccgacg cctgaatccc
     5761 agcgaatctg ctttttgccg tccttaagca tgtcgttgtt caagaattcc agcagcttct
     5821 tggcttccgg ggatccggac ttccactcga tgccgatgta cacgtcctcg gtattttcgc
     5881 gaaaaatgaa gacgttcagc ttctccgggt ggcggacagg ggagggaact ccctcaaaat
     5941 aacgtactgg ccgttcgcag gaatacagat caagaatctg ccgcaacgcc acgttcagcg
     6001 aacgaatccc gccgcctaca ggtgtcgtca gcggcccctt gatcgaaacg cgaaaatcgc
     6061 gaattgcttc gaccgtatcg ttcggcagcc actccttgaa cgtattgaat gctttttcgc
     6121 cggcgaacac ttcgtaccac gcaattcgtc gcttgccgcc gtaagcatgt tcaatcgcag
     6181 catcaaacac gcgccgcgat gctttccaga tgtcgcggcc cgtgccgtcg ccttcaataa
     6241 acgggatgat cggattcgcc ggcacctgca attcgccgcc ggcgtattca atcggcgttc
     6301 cgtccgacgg aactgcttgc ccgttgtaac tgctcttcat tgtgcccatc tcccggatcg
     6361 tgtttggccg cgtgaaggga agaacggccc tgttgcttcc ggcgcgtgtg gccgaatttg
     6421 gaaagatacc acaagcgtgt gtggagctga aatgatggga gacactttgc ttcgcacagc
     6481 gccggtccct tgcatgcgtc gagttgctgt aaagggttca ataacgggcg agcttgcagc
     6541 gcctggacgt gaaggtagaa ttaccggggc tttggctgat tcaagcttta acctcaagag
     6601 gagactcgaa tgatgctcga ctggaacgaa tatcagaaac agctgaacgg acgtatcgcg
     6661 gaaatcgcaa aactttcgac cgagaccgta aagggttacg cgatgctttc cagcgctaac
     6721 gccaagacca cacatctggg cgaaaaagtg cgccaattga tctgcctggc cgcggcggtc
     6781 acgctccgct gcgacggctg catcgtgttc cataccgacg ctgcgttgaa agccggcgcc
     6841 aataaggaag aaatcagtga agcgctaggc gtggcggtcg caatgaatgc aggcgcctca
     6901 atggtctatt ccgcccgcgt gatggacgcc gtagaagcaa agacctcccg agccgcagcg
     6961 tagcccagtc aagttgaact tgcgcaacgg tcaagcgccg ccggagcagg cctaatcgta
     7021 gtattcctgc cccaggtgcg tgatcaaatc ttcgcccatc atccaacgca gcgtgttttt
     7081 cagcttcatg gactgaatga acaaatcgtg ctcgggataa agatccggcc cggtcatggg
     7141 cgacttgaag tagaaactga gccattcctg aatcccgcgg aatccggcgc gttgcgccaa
     7201 atccagaaag atcaccaggt ccagcacaat cggcgcggcc aggatcgaat cgcgacacag
     7261 aaaatccact tt
//
LOCUS       DASTPM010000654         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_485072, whole genome shotgun sequence.
ACCESSION   DASTPM010000654 DASTPM010000000
VERSION     DASTPM010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485072"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1465)
                     /gene="zwf"
                     /locus_tag="VFR42_11525"
     CDS             complement(86..1465)
                     /gene="zwf"
                     /locus_tag="VFR42_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU0369836.1"
                     /translation="MNVPHSDALVFFGATGDLAHKKIFPSLQAMIKRGHLDVPVIGVA
                     KGGWNIDQLRARARDSVEKHGGGIDPAAFDKLCGLLRYVDGDYKDAATFQAIRKELGS
                     AKRPAHYLAIPPALFGLVVEQLTKARCTSGARVIVEKPFGHDLISAQELNRILLGTFD
                     EQHIFRIDHYLGKRAVRNMVFLRFENAILESFWNRNQVESVQITMAENFGVQGRGAFY
                     DQTGAIRDVVQNHLFQILVNLAMEPPVRTDSETIRDEKVKVLKAIQPLEAKNLVRGQF
                     RGYRKENGVAPDSKVETFAALQLNVDSWRWQGVPFYIRAGKSLPVTCTEIAVRLREPP
                     VVFPTCCRARNYFRFRVSPNVTAAFGLTVMDQQEKMIGEQVELLASHYPGAEETDAYE
                     RVLGDAMAGDATLFAREDYVEEAWRIVDPVLKAGIPVHEYEPGTWGPSEVNEKVSPPG
                     GWENPMAST"
     gene            complement(1476..>2432)
                     /gene="gnd"
                     /locus_tag="VFR42_11530"
     CDS             complement(1476..>2432)
                     /gene="gnd"
                     /locus_tag="VFR42_11530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU0369837.1"
                     /translation="KNGQECVVFDRSPKVVNDLVKEKASGATSLADLVTKLDRPRAIW
                     LMVPAAVVDETIGELSPLLERGDIIIDGGNSYYVDDIERAKGLRSLGIHYVDVGTSGG
                     IWGLERGYCMMIGGDTEIVQRLDPIFAALAPGIGNVPRTPGREKLGGTAEQGYLHCGP
                     NGAGHFVKMIHNGIEYGIMAAYAEGIGILKAADAGKSQSEVDAETTPLRDPEHYQYEF
                     NLADISEVWRRGSVIASWLLDLTATALATDAQLAKFGGRVSDSGEGRWTIKAAIDEGV
                     PVPVLTTALYERFSSRGDADYADRLLSAMRFGFGGHLEKSAK"
BASE COUNT          504 a          760 c          637 g          531 t
ORIGIN      
        1 cgccgtgatc agtggcaatc ccaatacgca tgttttctcc ttaggtggat tgaaaaccaa
       61 tgttccaaag attccgtcat tccattcacg tgctggccat cgggttttcc cagcctcccg
      121 ggggtgatac cttttcattg acctcactcg gtccccaggt cccaggctcg tattcgtgga
      181 ctggaatgcc ggctttcagc accggatcta caatacgcca cgcttcttct acataatcct
      241 cccgggcgaa caacgttgcg tctcccgcca tggcgtcacc gagcacgcgt tcgtatgcat
      301 ccgtttcctc cgcaccggga tagtggctcg ccagcaactc cacttgttcg ccaatcattt
      361 tctcttgctg atccatcaca gtcagaccaa aggcagccgt aacgttagga ctgacgcgga
      421 atcggaagta gttgcgggca cgacagcacg ttggaaaaac gacgggtggc tcgcgcaagc
      481 ggacggcgat ctcagtacac gttaccggca acgacttccc ggcccgaatg taaaacggta
      541 cgccttgcca gcgccaggaa tccacgttca actgcaaagc cgcgaaggtt tcgacttttg
      601 aatccggcgc cacgccgttt tccttccggt aaccacgaaa ctggccccga acaagattct
      661 ttgcctctag aggctgaata gccttcagaa ccttaacctt ttcgtcgcgg atcgtttcac
      721 tatcggtcct caccggtggc tccattgcca ggttcaccag gatctgaaac aggtgatttt
      781 ggacgacgtc ccggatagcg ccagtttgat cgtagaacgc cccccggcct tgaactccga
      841 agttttccgc catggttatc tgcacgctct cgacttgatt gcggttccag aacgactcca
      901 gaattgcatt ctcgaagcgt aagaagacca tgttccgcac tgcgcgtttc cctagatagt
      961 gatcgatgcg gaagatgtgt tgctcgtcga aagtgccgag cagaatccga ttgagttcct
     1021 gcgccgaaat gaggtcgtga ccaaagggtt tttcaacgat gacgcgagca ccgctggtgc
     1081 agcgcgcctt tgtgagttgt tccacgacga gcccgaagag ggcaggcgga atggcgaggt
     1141 aatgtgcagg ccgcttcgca gagcctagct ccttgcggat ggcctgaaag gtggcggcgt
     1201 ctttgtagtc accgtcaaca taacgcagca ggccgcacag cttgtcgaag gccgccgggt
     1261 cgatccctcc gccgtgtttc tcgacgctgt cccgtgcccg cgctcggagc tgatcgatat
     1321 tccaacctcc cttggccacg ccgataaccg gcacgtcgag gtggccccgt ttgatcatcg
     1381 cttgcaacga gggaaagatt ttcttgtgag cgagatcgcc cgtggcaccg aagaagacca
     1441 acgcatccga atggggaacg ttcatgccgt gctcctcatt tcgcggattt ttctaggtgg
     1501 cccccgaaac caaagcgcat cgccgaaagc agcctgtcgg cgtaatcagc gtcaccacgc
     1561 gatgagaacc gctcgtacaa ggcggtcgtt aacaccggca ccggcacgcc ttcatcgatg
     1621 gccgccttaa tcgtccaacg accttcgccg gaatcggaaa ctctgccgcc gaacttcgct
     1681 agctgggcgt ctgtcgcgag cgcagttgcg gtcagatcca gtaaccacga agcgatgacg
     1741 ctgccgcgcc gccaaacttc cgaaatatca gccaggttga actcatactg gtagtgctcc
     1801 ggatcgcgca acggcgtagt ctcggcatcc acctcactct gactcttccc cgcgtcagcg
     1861 gctttcagaa tgccgatacc ctcggcgtaa gcggccatga tgccgtattc gatgccgtta
     1921 tggatcattt tgacgaagtg cccggcgccg ttaggtccac aatgcaagta accttgttcg
     1981 gccgtgcccc cgagtttctc gcggcccggc gtgcgcggga cgttgccgat gcccggcgca
     2041 agcgcggcaa agatcggatc caagcgttga actatctctg tatcaccacc gatcatcata
     2101 cagtagccac gctccaagcc ccagatgccg ccgctcgtcc cgacatcgac gtaatgaata
     2161 cctaacgagc gcagtccctt cgcccgttct atgtcatcca cgtagtagga gttgccgccg
     2221 tcgataatga tatcgccacg ttcaagcagc ggtgatagct cgccgatcgt ctcatcgacc
     2281 accgccgccg gaaccatcag ccagatcgct cgcggccggt ccagcttggt caccaagtct
     2341 gccagcgaag tagccccact tgctttctct ttgacgaggt cgttgaccac cttgggtgaa
     2401 cggtcgaaca ccacacattc ctgtccattc tt
//
LOCUS       DASTPM010000655         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1263719, whole genome shotgun sequence.
ACCESSION   DASTPM010000655 DASTPM010000000
VERSION     DASTPM010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263719"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..517)
                     /locus_tag="VFR42_11535"
     CDS             complement(71..517)
                     /locus_tag="VFR42_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369838.1"
                     /translation="MATRIIPDEDKPSVAIEISLEKLLPDYDINEIEQPTPRDVDGVL
                     VSQGFRDLVDDARGVLLEIMSEPPPDPNASSQITDLDLTPPEAHPMEIAQVTGAICPG
                     SDEVYRPGLWIVLHDHKIPANQPLPASTVERVGHYTEKLVKRLDIE"
     gene            complement(545..973)
                     /locus_tag="VFR42_11540"
     CDS             complement(545..973)
                     /locus_tag="VFR42_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU0369839.1"
                     /translation="MKLRLLMLGKTRRPELRAIFDDYVKRINRSCPTEVNEVRDGDAA
                     IKKLDADRAATALLLDVGGKPQNSESLARWLAEQRDRGTREVIFVCGDADGFPEALRQ
                     RVLQKLSLSPMTYSHELARVMLAEQLYRAFAILSGSPYPK"
     gene            complement(970..1905)
                     /locus_tag="VFR42_11545"
     CDS             complement(970..1905)
                     /locus_tag="VFR42_11545"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0369840.1"
                     /translation="MSERFPFIAADLSSQRLVALAEKVAPAPTTLLITGESGSGKDHF
                     ARLIHELSPRRDAPFLKIDCTSLPPQLIESELFGHERGAFTGAVERKLGRLELGGKGT
                     IVLDEIAAFSPEAQGKLLRVLQERTFERLGGTDALKIDARLIALTSTDLHAAVNVGQF
                     REDLFFRLSVLPIVVPPLRDRPADIVPAAEHFLAALAPLHRKPAMQLSDSAARMLPAH
                     SWPGNFRELRNSVERAIVFSESEQLQPDDFPEHVRANAGNAAFLAGLRSLEDLEREAI
                     SRTLEATRYHITHSAGILGISRKTLLEKRKKYGLQ"
     gene            complement(1955..2266)
                     /locus_tag="VFR42_11550"
     CDS             complement(1955..2266)
                     /locus_tag="VFR42_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369841.1"
                     /translation="MQITIVRNSPDDTQTRQIKIFLDGESQGELMFGDSMTLDVSVGA
                     HTIRVDNTWNRKDLAIEIHAGESLQFLTKSSAGKFAWFLLGFLGAGPMQVSIEPLPSR
                     S"
     gene            complement(2311..2727)
                     /gene="rsfS"
                     /locus_tag="VFR42_11555"
     CDS             complement(2311..2727)
                     /gene="rsfS"
                     /locus_tag="VFR42_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU0369842.1"
                     /translation="MNLKKLPPAIRLTIEAMQNKKASAITVLDLSSLGTFTEYFVLCT
                     GFSTPQVQAICSEVEEFLYKEFQREPEHREGHRSADWALLDFGGFVVHVFSEQGRRFY
                     DLERLWRNVPRYEVADLPDAVVSEDHPRLRASGDHS"
     gene            complement(2724..3440)
                     /gene="nadD"
                     /locus_tag="VFR42_11560"
     CDS             complement(2724..3440)
                     /gene="nadD"
                     /locus_tag="VFR42_11560"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU0369843.1"
                     /translation="MTTAQAHRTAAKHAPRRIALFGGSFDPIHNGHLAVARAADRRFN
                     FDEIHFIPASRPPHKLKQHLAPFPHRYAMVALACTEHPHFVPSLSEAGDDFAGNQLHY
                     SVDTVRYFRHSYHGHGDRIYFIIGADAFLDIPMWKEYESLLGLCDFIIANRPGIRADA
                     LKLVIPPDLLAGPNDKKETDPPSQLIAQLRRTSIYLLENVSSDVSATDIRRRAHKGQA
                     IHGLVSARVEEYIVKQGLYR"
BASE COUNT          733 a          860 c         1248 g          697 t
ORIGIN      
        1 gggaagtcga gaaacgtcaa attgtgcgag tcgtttggac acgcgagtcg gggcgggtca
       61 ggaggttagt tcactcgata tccaagcgct tgacgagttt ctcggtgtaa tgccccacgc
      121 gttcgaccgt agaagcggga agcggttgat tggcgggaat tttgtgatcg tgaagcacaa
      181 tccacaagcc agggcggtag acttcgtccg atccgggaca gattgcgcca gtgacttgag
      241 cgatttccat aggatgagct tcgggcggcg tcaaatcgag atcggtgatt tgcgaactgg
      301 cgttgggatc ggggggaggc tccgacatga tttccagcaa gactccccgg gcatcatcga
      361 ccagatcgcg aaagccctgc gagacgagca cgccgtctac gtcacgagga gtgggttgtt
      421 caatttcatt gatgtcgtaa tcgggcagca gcttttccag cgaaatttcg atagcgacag
      481 acggcttgtc ttcgtcggga atgatgcggg tggccatggc agataaggtt ctacactatg
      541 ccgctcattt gggatagggg ctgccggaaa gaatggcgaa agcgcgatag agctgctcgg
      601 cgagcatgac gcgggcgagt tcgtgggaat aagtcatcgg gctaagcgaa agcttttgca
      661 ggacgcgctg gcgtagagct tcggggaaac catcggcgtc gccgcagacg aaaattactt
      721 cgcgggtgcc gcggtcgcgc tgttccgcga gccatcgcgc cagcgattcg gagttctgcg
      781 gcttgccgcc gacgtcaagc aatagcgcgg tagcggcgcg gtccgcgtcg agcttcttga
      841 tggcggcatc tccgtcgcgc acttcgttga cttcggtggg gcaggagcgg ttgatgcgtt
      901 tcacgtagtc gtcaaaaatg gcacgaagtt cgggacggcg tgttttgccg agcatgagca
      961 ggcgaagttt cattgcaggc catacttttt gcgcttttcg aggagcgttt tacggctgat
     1021 gccgagaatg ccggcggaat gcgtgatgtg gtagcgcgta gcttcgagag tgcgagagat
     1081 ggcttcgcgt tcgaggtctt cgagcgaccg caagccggcc aggaatgcgg cgttgcccgc
     1141 attggcgcgg acgtgttccg gaaaatcgtc gggttgcaac tgctcggact ctgaaaaaac
     1201 gatagcacgt tcgacggagt tgcgaagctc gcgaaaattg ccgggccagg aatgcgcggg
     1261 gagcatgcgg gcggcggagt cggagagttg catcgcgggc ttgcgatgta gaggcgcgag
     1321 cgccgcgaga aaatgttcgg ccgcgggaac gatgtcggcg gggcgatcgc gaagcggcgg
     1381 gacaacaatg ggcagcacgc tcaggcgaaa gaagagatct tcgcggaact ggccgacgtt
     1441 gaccgcggcg tgaaggtcgg tgctggtgag cgcgatgagg cgcgcatcga ttttcaacgc
     1501 atccgtgccg ccgaggcgct cgaaggtgcg ctcctgcagc acgcgcagta gtttgccctg
     1561 tgcttcggga gaaaaggcgg cgatttcgtc gagcacaatg gtgcctttgc caccgagttc
     1621 gaggcggcca agttttcgct ctacggcgcc agtgaacgca ccgcgctcgt ggccgaaaag
     1681 ttcgctctcg atcagctgag gcggaagcga cgtgcagtca attttcagga aaggagcatc
     1741 gcggcgcggg ctgagctcgt ggatgaggcg cgcgaagtga tctttgccgg agccggattc
     1801 gccggtgatg agcagcgtgg tcggagcggg cgccactttt tcggcaagcg caacgagccg
     1861 ctgcgacgac aggtcggcgg cgataaaagg aaagcgctca gacatacaga aattgtagcg
     1921 agaacgcgag actcgctgga gtgcgccaga gaggctacga gcgagagggt aacggttcga
     1981 tggagacttg catggggccg gcgccgagga agccgaggag gaaccaggcg aattttccgg
     2041 cggagctctt ggtgaggaac tggagacttt cgccagcgtg aatttctatg gctaggtctt
     2101 ttcggttcca ggtgttgtcg acgcgaatgg tgtgggctcc cacgctgacg tcaagagtca
     2161 tagaatcgcc gaacatcaat tcgccctgac tctcgccatc cagaaaaatt ttgatctggc
     2221 gagtttgcgt gtcgtcgggc gaattacgca cgatggtgat ctgcacggtg gcggagccgg
     2281 aatgtccagg cggcggcacg gcgctggcga tcaggaatgg tcgccggaag cacgcaggcg
     2341 cggatgatcc tcggagacga ccgcgtcggg cagatcggca acttcgtagc gcggcacgtt
     2401 gcgccacaga cgttcgaggt cgtagaaacg gcggccttgt tcgctgaaaa cgtgcacgac
     2461 gaagccgccg aagtcgagta gcgcccagtc cgcggagcgg tggccttcgc ggtgttcggg
     2521 ttcgcgctgg aattccttgt acagaaattc ctcgacttcg ctgcagatgg cctgcacttg
     2581 tggcgtacta aagccggtgc acagcacaaa atactcggtg aacgtaccaa ggctgctcaa
     2641 gtcgagtacg gtgatagcgg aagctttttt gttctgcatc gcctcgatgg tgaggcggat
     2701 cgctggggga agctttttca gattcacctg tataggccct gtttaacaat gtattcctcc
     2761 acgcgcgcgg aaacaagtcc atgaattgcc tgccccttgt gagcgcggcg gcggatatcg
     2821 gtggcggaaa cgtcactgga aacgttttcc agaagataga tggaggtgcg gcgcagttgg
     2881 gcgatgagct gcgagggcgg gtcggtttcc tttttgtcgt tgggaccggc cagcagatcg
     2941 ggtggaatga ccagcttgag agcgtcggcg cgaatgccgg ggcggttggc gatgatgaaa
     3001 tcgcagaggc cgaggagcga ttcgtattcc ttccacatgg gaatgtcgag aaaggcgtcg
     3061 gcgccgatga tgaaataaat ccgatcgccg tggccgtggt aggagtggcg gaagtagcgc
     3121 acggtatcca cggaatagtg caactggttg ccggcgaaat cgtcgccggc ttcggagagt
     3181 gatggcacga agtgcggatg ctccgtgcag gccagtgcga ccatggcgta gcggtgcgga
     3241 aaaggagcga gatgctgctt gagcttgtgc ggcggccggc tggccgggat gaaatggatt
     3301 tcgtcgaagt tgaagcggcg gtcggcggcg cgggctaccg cgagatgacc gttgtgtatg
     3361 ggatcgaaag agccgccaaa aagcgcgatg cggcggggag cgtgttttgc ggcggtgcgg
     3421 tgagcttgcg ctgtggtcaa cgaggctcct gagagaagct aaaacatttt cgcatgaagg
     3481 aagttgacag tcgacggtca aaggcggaaa ggaaatggtc gctgagaagc agaaggcc
//
LOCUS       DASTPM010000656         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_946410, whole genome shotgun sequence.
ACCESSION   DASTPM010000656 DASTPM010000000
VERSION     DASTPM010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946410"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFR42_11565"
     CDS             <1..902
                     /locus_tag="VFR42_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU0369844.1"
                     /translation="IRWIYWILIPLTLAFMVLHNAFDLLAKIRRGVHRSDTPERVVRM
                     NIWFRIAHWGVMLSFPTLVYTGFALKFPEQSWARPLLQWEEHTSFRGSLHRLAAIVLI
                     AATLYHFVHLAVNRRDRLFLHAMLPKWQDASDLIAVMLHNLGLRQDEPRFAKFNYAEK
                     LEYWAFLWGTAIMTVSGFLLWFNNFALRHFPKWVSDAATAVHWYEALLATFSILLWHF
                     YMVIFDPLVYPMDLAWLTGKVPAEHYRHSRPAYFRALAQSKSAQAPQTANANANATTT
                     NASADATERPADAVIKFPSDDHD"
     gene            complement(975..1337)
                     /locus_tag="VFR42_11570"
     CDS             complement(975..1337)
                     /locus_tag="VFR42_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0369845.1"
                     /translation="MILEFSKRTVQPGVTVLAMRGSIHCGPECARLEREVDGLIAARD
                     TRVIFDMEGVTHADSAAIGAIVRSFGKLRDAGGGLRIAAAQSMVDHSLKLTKVDKVIE
                     IFPTVQQAAQGFAPPNAN"
     gene            complement(1334..1717)
                     /locus_tag="VFR42_11575"
     CDS             complement(1334..1717)
                     /locus_tag="VFR42_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369846.1"
                     /translation="MSDWVHIQADSGEQLMQLHHFSIVKQQPSGNVTFAITVKEFAVP
                     PPGQRLRFYAEADKPVNQSTAAFVPCGWGHSIFSALGDCVRLIRQFPYQSETPGEGRA
                     ASQSSGSQSSGNPAAGTSGTGTQSK"
BASE COUNT          490 a          674 c          687 g          526 t
ORIGIN      
        1 cgattcgctg gatttattgg attttgattc cgctgaccct cgcgtttatg gtcttgcaca
       61 atgcgttcga tctgctcgcc aaaatccgtc gtggcgtgca ccgctcggat actcccgagc
      121 gcgtcgtgcg catgaatata tggtttcgca tcgcgcattg gggtgtgatg ctcagcttcc
      181 ccacgctcgt ctataccggc ttcgcgctca aatttcctga acaatcgtgg gcgcgcccgc
      241 tgctgcaatg ggaagagcac acatcatttc gcgggagctt gcaccggctc gccgcaatcg
      301 tgctgattgc cgcaactctg tatcactttg tgcatctggc ggtaaatcgg cgcgaccgct
      361 tgtttctcca cgccatgctg cccaaatggc aagacgcttc ggatttaatc gcggtcatgc
      421 tccacaacct tggccttcgc caggacgagc cacgcttcgc aaaatttaat tacgcggaaa
      481 agctggaata ttgggccttc ctgtggggca ccgcgatcat gaccgtttcc ggttttctcc
      541 tctggttcaa taatttcgcc ctgcgccatt ttccaaaatg ggtttccgac gccgccacgg
      601 ccgtgcattg gtacgaagcg ttgctggcga cgttctccat tttgctctgg cacttctaca
      661 tggtgatttt tgatcctctg gtttatccga tggatctcgc ctggctcaca ggaaaagtcc
      721 ccgccgaaca ttatcgccac tctcgcccgg cgtatttccg cgccctcgcg caatccaaat
      781 cggcgcaagc tccgcagacc gcaaacgcaa acgcaaacgc caccaccacg aacgcgagtg
      841 ccgacgctac cgagcgacct gcggacgcgg tgatcaagtt cccatccgac gaccacgact
      901 gagaaatatt ctctttaaga ggacggctac ggagctagcc ttgagcgcga ctagcggaat
      961 tggtcgcttc tgatttagtt tgcgtttggc ggagcaaatc cttgcgcggc ctgctgtacg
     1021 gtgggaaaaa tttcgatgac cttatccact ttggtgagtt tcaaactgtg atccaccatg
     1081 gattgcgcgg ccgcgatgcg cagcccgccg ccagcgtccc gcagcttccc gaagctgcgc
     1141 acgatcgccc cgatcgctgc gctgtccgca tgggtcacgc cttccatgtc gaaaatcacg
     1201 cgcgtgtcgc gcgccgcaat cagtccgtcc acttcgcgct ccagccgcgc gcattccggg
     1261 ccgcaatgga tgctgccgcg catggccagc acggtcactc cgggttgcac tgtgcgcttg
     1321 ctgaattcca ggatcatttg ctttgcgttc ccgtgcctga agtgcccgcg gctgggtttc
     1381 ctgaagattg cgaacctgaa gattgggaag cggcgcggcc ttcgccgggc gtttcgctct
     1441 gatagggaaa ctggcggatc aggcgcacgc aatcgccgag cgccgaaaat atcgagtggc
     1501 cccagccgca aggcacaaat gcggccgtgc tttgattgac gggtttgtcg gcttcggcgt
     1561 agaagcggag gcgctggcct gggggcggga cggcaaattc tttcacggtg atcgcaaatg
     1621 tcacatttcc ggagggttgc tgcttgacga tggaaaagtg atgcaactgc atcaactgtt
     1681 ccccggaatc agcctgaatg tggacccaat cgctcattcg cgcagtccta gcggccggtg
     1741 aaggcgcaaa gcgggtggaa gcgaaagcaa gtaacagcgc ggatagtagg ccagcgtggc
     1801 agccaagtca atctcgacgg gccgtcggga tcaacggaag gacgatgggt aggcgatgat
     1861 gaggggacga tgagggaatg atcaggcggc ttggtcaagc gcgatggagc aatccgggat
     1921 ctcgctcaaa tccatatatt cgccaagctc cacccagtgg cgcacggcca taaaacccgc
     1981 tgattcacct atccagccgc aatggcagtg gaggtcaaaa atgtgcgctt ccgagggttc
     2041 gaggtttttt tgggagcagg aacagacgga tgcgagatac ccgcggcagg cagggcaggc
     2101 aaacagaaag aaatgtgcgt aggcttcttt tctcacaacg ccgactatcc tctaagaggg
     2161 gtgaagtggc ggcagcatat cacaggcgga aaggaatacg ctctatcgtt gttgcaaagt
     2221 cacggctaga aatgtcacgg ctgtgaaccg caggtggtga cgacctggct tttttgctgg
     2281 tcgaagacaa tatattcgcc tggcgaatgc agggcgagct cttctatgcg aacggtggct
     2341 tttggaaggt cttctacggc ctcaatccaa atgggat
//
LOCUS       DASTPM010000657         4295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_571820, whole genome shotgun sequence.
ACCESSION   DASTPM010000657 DASTPM010000000
VERSION     DASTPM010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571820"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..2248
                     /locus_tag="VFR42_11580"
     CDS             86..2248
                     /locus_tag="VFR42_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0369847.1"
                     /translation="MNSPEHKFVIEPAIEPERYEFNSPSLHQFALARRDFFKLLGTGI
                     TVFAVNQVALATQETAPGPRIQPPQLPKEITSWLHIGDDGSFTAFTGKVEVGQNARTS
                     LTQSVADELRVPFSSVRMVMGDTELTPFDMGTFGSRTTPTMVPQLRRVAEAARNLLVE
                     VAAKQWNVAPAVLKAANARVTDPASGKSLTYAELARGKTLAQFLPDADPTTPPSEWTI
                     AGQPLPKVDARDFVTGKHQYTADLRPDKLLYAKILRPPSFGATLASCDVKAAAAMPDV
                     VVVVDGNFVGAAAPTERQAETAVAAIRAQWNESPQKISDREIFDYLKRNTSADSDRRN
                     EKQPKGSISDGLASATRKLESVYTVAYIAHAPLEPRAAVAEWKNSKMTVWTGTQRPFA
                     VRTEVANVLRIPESTVRVIVPDTGSAYGGKHTGDAAVEAARLAKSANRPVKLVWTREE
                     EFTWAYFRPAGLIEVKSGLDSNGKLTAWEFHNYHSGTSGIETPYDVANQLVEYHSVPL
                     VLRCGSYRGLAATANHFARETHMDELARASNLDPLEFRLRNLSDKRLRAVLEAAAQKF
                     GWPPKKSKEGQGFGIAAGNEKGSYVATCAEVISDPKANKLQVIRLVTAFECGAVVNPD
                     GLRNQVIGANIQGLGGALYEAIRFENGRINNPHFASYRVPRFRDLPAIEAVLLDRKDI
                     PSAGAGETPIMAVAPAVGNALFDATGVRRRAMPLASGA"
     gene            2617..3162
                     /locus_tag="VFR42_11585"
     CDS             2617..3162
                     /locus_tag="VFR42_11585"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0369848.1"
                     /translation="MIPYGGKDLADAFRTVRKNTIQVAEDIPESKYDFVAAPDVRPVS
                     KMLTHVAIATRIWEEVHGKKLTTLVGFNFFGLMDEFNAEENQPRSKAEIVQLLRAEGE
                     KFASWVETLTPEFLAETVAEPDGKTLKTRFERLLGAKEHEMHHRAQLMLIERQLGIVP
                     HLTRQFQERMAQMKQQRAANA"
     gene            3330..>4295
                     /locus_tag="VFR42_11590"
     CDS             3330..>4295
                     /locus_tag="VFR42_11590"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0369849.1"
                     /translation="MKRPIRFLAALSAAALLFIPGAAPTRAQQPAANGPATFDMTLAK
                     PESVGFSSERLERLHGLMQQTVDQKQLPGGVTILARHGKVIDYRVYGVKDVASSAPLT
                     KDAIFRAFSMTKPITAVAMLQLYEQGKWLPSDPISKFIPEFGHLKVSKGADASGNMVL
                     EDPIRPPTMMELMTHTAGFTYGFFGNTLVDKEYAKANLFQSKSLQEFIDKLAKLPLMY
                     QPGTRWSYSVSMDIQGYIIEKLSGQSLPDYMQEHIFKPLGMKDTGFYVPAEKRSRLVT
                     LYRGNDKGEMVPADNGFGGDYASEPAMASGGGGSVSTAEDYFRFAV"
BASE COUNT          928 a         1497 c         1047 g          823 t
ORIGIN      
        1 agggaaatat ctgccgctgc ggcactcacc cgcgcatcat tctagccgtg caagaagcag
       61 cgaaggcatc tcgggtacac cgctcatgaa ttctccggaa cataaattcg tcattgaacc
      121 agccattgag cccgagcgct acgagttcaa ctcgccctcg ctccatcagt tcgccttagc
      181 ccgccgcgac ttcttcaaac tcctaggcac tggcatcacc gtctttgcgg taaaccaggt
      241 cgccctcgca actcaggaga ccgctcccgg cccgcgcatc cagccaccac aacttccgaa
      301 agagattacc tcctggctgc acatcggcga cgatggctct ttcaccgcct tcactggcaa
      361 agtcgaagtc ggccagaacg cccgcacctc gctcacgcaa agtgtcgccg acgagctcag
      421 ggtgcctttt tccagcgtgc gcatggtcat gggcgatact gaactcactc cctttgacat
      481 gggcacgttc ggcagccgca ccacgccgac catggttccg caactacgcc gcgttgcaga
      541 agccgcgcgc aatctgctgg tcgaagtcgc tgccaagcaa tggaacgtgg cgcccgccgt
      601 gctcaaagcc gccaacgcca gagtcaccga tcccgcatca ggaaaatccc tcacctacgc
      661 cgagctcgcg cgcggcaaaa ctctcgccca attcctcccc gatgccgatc ccactactcc
      721 gccgtctgaa tggactattg caggacagcc cctccccaaa gtggatgctc gcgacttcgt
      781 caccggcaag caccaataca ccgccgacct ccgcccggat aaactcctct acgccaaaat
      841 tcttcgccca ccgtcgtttg gcgccacgct agcctcctgc gatgtcaaag ctgccgctgc
      901 catgccggat gttgtcgttg ttgtcgacgg caactttgtc ggcgctgcgg ctcccaccga
      961 acgacaggcc gaaaccgctg ttgctgcaat tcgcgcccag tggaacgaat ccccgcaaaa
     1021 gatttccgat cgtgaaatct tcgactacct caaacgcaac acgtccgccg actccgatcg
     1081 ccgcaacgag aagcagccaa agggctccat ttccgacggc cttgcctcag ccacgcgcaa
     1141 actcgaatcc gtctacaccg ttgcctatat cgctcacgca ccccttgagc cgcgcgccgc
     1201 cgtcgccgaa tggaaaaaca gcaagatgac cgtctggact ggcacccaac gtccttttgc
     1261 cgtgcgtact gaagttgcca acgtgctgcg cattcccgaa agcaccgtac gggtcatcgt
     1321 tccggacact ggctccgcct acggcggcaa gcacactggt gacgcagcgg tcgaagccgc
     1381 ccgcctcgcc aaatccgcca atcgtccggt caagctcgtg tggacacgcg aagaagaatt
     1441 cacctgggcc tatttccgtc ccgccggtct catcgaagtg aaaagcggtc tcgactcaaa
     1501 cggcaagctt acggcctggg aatttcacaa ctaccattcc ggcacgtccg gaatcgaaac
     1561 tccctacgac gtcgcgaacc aactcgtcga gtatcactcc gtgccgctcg ttctccgctg
     1621 cggctcctat cgcggactcg cggccaccgc caaccacttc gctcgcgaaa cgcacatgga
     1681 cgaactcgcc cgcgccagca atctcgatcc gctcgaattc cggctccgta atctgtccga
     1741 caaaaggctc cgcgccgtcc tcgaagccgc ggcgcaaaag tttggctggc cgcctaagaa
     1801 atcgaaagaa gggcagggct ttggcatcgc cgccggaaac gagaagggaa gttacgtggc
     1861 cacctgcgcc gaggtcatca gcgaccctaa agccaataag cttcaggtca ttcgcctagt
     1921 cactgccttc gaatgcggag ccgtcgtcaa tcccgacggc ctacgcaacc aggtcatcgg
     1981 cgccaacatt cagggactcg gcggcgctct gtacgaagcc attcgattcg aaaacggccg
     2041 catcaacaac ccgcatttcg caagctatcg tgtcccgcgc ttccgcgatc ttcctgcaat
     2101 cgaagccgtc ttgctcgatc gcaaagatat cccctccgcc ggcgccggcg aaactcccat
     2161 catggcggta gcccccgccg tcggcaacgc gcttttcgat gccaccggag tccgccgccg
     2221 tgctatgccc ctcgcttctg gcgcctgacc cctccgccca gaatttgcat tgaactcaca
     2281 acgggcttgg cgcaatcttg cgcaagtgca cggctctccc gggttgctcc acccacgagg
     2341 atgcctcaaa tgccctgcgc ctctcgatcc cgagcgctgc accatcaagc aagccgccgc
     2401 tgaccgcaag agctccgccg cctgcctgca aatccccaag aacgcacttt cggcatcgcc
     2461 gggtccggtt aaatcgccct gcccacgccg aatcgatcca aagattgctt gcctaatccc
     2521 gatcgccttg aaaaaatacc gttaccaccc aggtgcccat gcatcgaatc gttcggctct
     2581 gggttccggg cgtaactcaa cacactgagg tgaatcatga ttccttacgg cggcaaagat
     2641 ctggccgatg cgttccgcac cgtccgcaaa aacaccattc aggtagcgga agacatcccc
     2701 gaatcaaaat atgatttcgt cgcggctccc gacgtccgcc cggtcagcaa aatgctcact
     2761 cacgtcgcca tcgccacgcg catctgggaa gaagttcacg gcaagaagct gaccactctc
     2821 gtcggcttca actttttcgg gctcatggat gaattcaacg ccgaagaaaa ccagccccgt
     2881 tccaaggcgg aaattgttca attgctccgc gccgaaggcg aaaagtttgc ttcctgggtg
     2941 gaaacactga ctccggaatt cctcgcggaa acggtcgccg aacccgacgg aaagacactg
     3001 aagacgcgct tcgaacgcct cctcggcgcc aaagaacacg aaatgcatca ccgcgcccag
     3061 ctcatgctca tcgagcgcca gcttggtatc gtgccgcatc tcactcgcca atttcaggag
     3121 cgcatggccc aaatgaaaca gcagcgtgcc gcgaacgctt aaagtccctc agtttaaatc
     3181 catttcaaat ccgctcgacg gcggtcgcac ctttcgcggc cgccgtgagc cctcttcgac
     3241 cctcgggctt gccactctcg ctgtgagttt cactgtataa tcctccccgt ttttctggct
     3301 tgccattcgt gttcttcggg gagaaaaaaa tgaagcgacc aatccggttc ctcgcagcac
     3361 tgtcagcggc agctttgctt tttattccag gcgcagcacc aactcgtgcc cagcaacccg
     3421 ccgccaacgg ccccgccacc ttcgacatga ctctcgccaa gcccgagtcc gtcggcttct
     3481 cctccgagcg gctcgagcga ctccatgggc tgatgcagca aaccgtggac caaaagcagc
     3541 tccccggcgg cgtcacgatt ctcgcccgtc acggcaaagt catcgattat cgcgtctacg
     3601 gggtaaaaga cgtcgccagt agcgcgccgc tgaccaaaga cgccatattc cgcgccttct
     3661 ctatgaccaa gcccatcact gccgtcgcca tgctccaact ctacgaacag ggcaaatggc
     3721 ttccttccga tcccatctct aaattcattc ccgaattcgg acatctcaaa gtctcgaagg
     3781 gcgccgatgc ttccggcaat atggtcctcg aagatcccat tcgccctccg accatgatgg
     3841 agctcatgac ccacaccgcc ggcttcacct acggcttctt cggcaacact ctggtggaca
     3901 aggaatacgc aaaagcgaac ctcttccaat cgaaatcttt gcaggaattt atcgataagc
     3961 tcgccaaact tccgctcatg tatcagcccg gcacccggtg gtcctacagc gtctccatgg
     4021 acattcaggg ttacatcatc gagaagcttt ccggccagtc gctccccgat tacatgcagg
     4081 aacacatctt caagcctctc ggcatgaagg acaccggctt ctacgttccc gccgaaaagc
     4141 gcagccgctt ggtcaccctc tatcgcggca atgacaaggg cgaaatggtt cccgcggaca
     4201 acggtttcgg cggcgactac gcttccgaac ccgccatggc gtcgggcggc ggcgggtcag
     4261 tttccacggc cgaggattat tttcgtttcg cggta
//
LOCUS       DASTPM010000658         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_626114, whole genome shotgun sequence.
ACCESSION   DASTPM010000658 DASTPM010000000
VERSION     DASTPM010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626114"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFR42_11595"
     CDS             <1..308
                     /locus_tag="VFR42_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0369850.1"
                     /translation="PTPGSRKRPDGKELHWQTLNLQDDASGLLPFYIQWSENSVHPSV
                     DAPSGCQLLRFELLTPDPAPLAMKLAKLTLSAPVKKAAAPQLHAVIQGPKGALSVTS"
     gene            856..1527
                     /gene="infC"
                     /locus_tag="VFR42_11600"
     CDS             856..1527
                     /gene="infC"
                     /locus_tag="VFR42_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU0369851.1"
                     /translation="MELGPEPAIRVFRRFYHLGFQGLYLQTLTGQGGTIADRNFRPQS
                     DRVRINERIRAREVRVIDEDGGQLGVMQPFEALRVARERGLDLVEISPTADPPVCKIT
                     DYGKFLYQANKRAHEQRKSSRGSALKEVKFRPNTAEHDFQVRKNQIIRFLGEGHKVRA
                     MIFHRGREMAHQEVGRAKMSRLLGELGDQGQIETPPRMEGNVLVALLAPKKGSGVPAP
                     QRPAN"
     gene            1676..1900
                     /gene="rpmI"
                     /locus_tag="VFR42_11605"
     CDS             1676..1900
                     /gene="rpmI"
                     /locus_tag="VFR42_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU0369852.1"
                     /translation="MPRNASKPKRKLKTHRGAAKRFKLTATGKLLRMHSGKRHLLGTK
                     AAKRMRKLKKLTQVNHADKAKTMQMLPYGL"
     gene            2299..2676
                     /gene="rplT"
                     /locus_tag="VFR42_11610"
     CDS             2299..2676
                     /gene="rplT"
                     /locus_tag="VFR42_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU0369853.1"
                     /translation="MARVKRGTKRRARRKKYLKRTKGFFLTKSKLYQSAQEAANRADR
                     YAFRDRRAKKRQYRRLWIQRIGAAARLNGITYGQLIHGLKAAGVELDRKVLADIAVKD
                     AAGFAQLAEKAKAFAPRPAAKKA"
     gene            2846..>3844
                     /gene="pheS"
                     /locus_tag="VFR42_11615"
     CDS             2846..>3844
                     /gene="pheS"
                     /locus_tag="VFR42_11615"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU0369854.1"
                     /translation="MPENSHEKTLAALGVTQPEQVAPLFTQLLAQATADLQAANSSET
                     FEKFRVHWIGRKSGVLTLATDNWLKPASPELKRAVGQELNKFKAQLESQMEVRRAAIE
                     SGAEEAVSAKDRIDLSLPGVVRPIGSRHLIRQVFQEIEDIFVSIGFSVVEGPEIETPY
                     YNFEALNIPEFHPVRDDMDTFYVEGANDAGVKTPPGSLLLRTHTSPVQIRTMEKQKPP
                     VRVIVPGKVYRRDNPDATHSFMFHQIEGLAVDTDITFCDFTGTIEYFIKQYFGPSMKA
                     RFRPSYFPFTEPSVEFDVSCIFCGGSGTGASGATCGKCKGAGWIELFGAGMVDPAVY"
BASE COUNT          799 a         1183 c         1052 g          810 t
ORIGIN      
        1 ggcccacgcc cggttcccgc aaacgtcccg acggcaaaga acttcattgg caaacgctca
       61 acctgcaaga cgatgcgagc ggcctcttac cgttctatat ccagtggagc gagaactccg
      121 tccacccatc cgtcgatgcg ccctccggct gccagctttt gcgcttcgag ttgctcacgc
      181 ccgacccagc cccgttggca atgaagcttg caaagctcac gctttccgct cccgtcaaaa
      241 aagcagccgc cccgcaactc cacgctgtca tccagggccc caaaggtgct ctttccgtca
      301 cctcctgacg gcgacgtaac cgtttgttcg ccgtagtacc tattgttctt ccaggagagt
      361 gctttcttcc tggttgctgc cggcgccgct tggcgcctgt tcaccaaaga cttgcccgcc
      421 ttacccagtt accgtacagc ggcctactac ctgtgcgtgg tggcgtttct gcgaatcgtc
      481 ctgcgttgcg ttcctggcca cggcttcctt tctcagtagc gcggaaattt cttcccgaaa
      541 tgaaacgctt tcactcccat cgccactact tgtcagcacc aacaactcag gggcagaggc
      601 ctggttggtc caaaccgagt tgaaggcgcc tgggggcact gctaggggct gagcggtcac
      661 cacactggcc cgtgccccct cttttgtttt ttaccggggc ggtgtttggg cctctaccat
      721 ctttcgcggt cgtcgggtca gtagtcagcc gctgtttccc aaacggcttg ccgtcggtgc
      781 cgctcgccat gctatacttt aaagtccgtc ctcatgtggc ttatcgccat gtagaggccg
      841 cttgcgtgtt tggcagtgga attgggcccg gagccggcta tccgtgtctt ccggcgcttt
      901 tatcatctcg ggtttcaggg tttgtactta caaaccctga cggggcaagg gggaactatc
      961 gcagatagga atttccggcc gcagagcgat cgcgtccgca tcaatgaacg gattcgagct
     1021 cgcgaagtcc gggtgattga cgaagatggc ggtcagctcg gcgtcatgca gccttttgaa
     1081 gcgcttcgcg tggcccgcga acgcggattg gatctggtgg agatttctcc cacggccgat
     1141 cctccggttt gcaagatcac cgattacggc aagtttcttt accaagcgaa caagcgcgcg
     1201 cacgagcagc gtaaaagcag ccgtggctcg gctttgaagg aagtcaagtt ccggcccaat
     1261 accgcggaac acgattttca ggttcgcaag aaccagatca tccgcttcct tggagaaggt
     1321 cacaaagttc gcgccatgat ttttcatcgt ggccgcgaaa tggcgcacca ggaagtcggc
     1381 cgcgctaaga tgagtcgcct gctcggcgaa ctcggcgatc agggacagat cgaaactcca
     1441 ccgcgcatgg aagggaacgt ccttgtggcg ctcctcgctc ctaaaaaggg cagcggcgta
     1501 ccggctccgc aaaggccggc gaattaaggg gcgaagttgt caggggtata cccttttccc
     1561 tggccgcgaa atcggaacta cggctccggt cggtctgtaa attgatgatt ttaggaagct
     1621 tgttgagttc aagccattct aaacaggtat ctgtagcaaa aggaaaggtc tcgcaatgcc
     1681 tcgcaacgct tcaaagccca aaagaaagct caaaacccat cgcggcgccg ccaagcgctt
     1741 caagctcacc gctaccggca agctgctccg catgcattcc ggcaagcgcc acctgctcgg
     1801 caccaaggcc gccaagcgca tgcgcaaact caagaagctc acacaggtca atcacgccga
     1861 caaggccaag accatgcaga tgcttcctta cggcctctag gattatggga aaagtttctt
     1921 cgtaagggcc cgctacatcg tgcccttgct ttgaaaagcg gaatgcaaat tccgcaagcc
     1981 gctcgcgaaa tcagcgagca gcattcgtgc caggcagcgc ttcacgggtg cctgccggcc
     2041 gaaaccgctc cggccttgcc ggagcaatgt agcggcccct tcaggtggcc gcttttccga
     2101 gcgatcggaa aatggcgaat tcaacttcgc cgctaccgca ccaggggaag caaacccgtt
     2161 gcgctacacg catttcgcgc tgcgcttttt gcgctgcgct tttcgcgtag ccgcaacccg
     2221 ttctgtttcc aaaacatttt gaaccgctga gacgctcttc gctttttgcg agtcgcctcg
     2281 gcacaaggag acaaacccat ggcacgcgta aaacgcggta caaaacgccg ggcccggcgc
     2341 aagaagtacc tcaagaggac caaaggcttt ttcctcacca agagcaagct gtaccagtct
     2401 gcgcaggaag cggccaatcg cgccgatcgt tatgcgttcc gcgatcgccg cgccaagaag
     2461 cgccagtatc gtcgcttgtg gattcaacgc atcggcgcag ccgcccgctt gaacggcatc
     2521 acctacggtc agctgatcca cggcctcaaa gccgccggag tcgaactcga ccgcaaagtt
     2581 ctcgccgaca ttgcggtgaa ggacgccgct ggcttcgcgc aactcgctga aaaggccaaa
     2641 gcattcgcgc ccaggcccgc cgcgaagaaa gcctgacgcg gcaaggatag cggaaaggcg
     2701 aatccgtcta ttccgagcgg agcgaggaat ctctttttgg gttcctcgcc cgtattccgg
     2761 tgcacgattt cgccagcttc ggtttcgtag atcttgtgtg gcgctggcgg cgtgcgcacc
     2821 ctgtcctgta atctatctgt tccctatgcc tgaaaattcc cacgagaaaa cgctcgccgc
     2881 actcggcgtg acgcaacccg agcaggttgc gccactcttc acgcagttgc tcgcgcaggc
     2941 gactgctgat ctgcaggcgg caaattcctc ggaaaccttc gagaaattcc gcgttcactg
     3001 gatcggcaga aaatccggcg tgctcacgct agccaccgac aactggctga agccagcttc
     3061 gcccgaactg aagcgtgcgg tcggccagga actcaacaaa ttcaaagcgc agctcgaatc
     3121 gcaaatggaa gttcgccgcg ctgccattga atccggcgct gaggaagcgg ttagcgcgaa
     3181 ggaccgcata gacctttcac tgccaggcgt ggtacgtccc atcgggtcgc ggcatttgat
     3241 ccgtcaggtt ttccaggaga tcgaagacat cttcgtttcg attggttttt ccgtggtcga
     3301 aggaccggaa atcgaaactc cgtattacaa cttcgaggcg cttaatattc cggagttcca
     3361 tccggtgcgc gacgacatgg atacgtttta tgtcgaaggg gcgaacgatg ccggcgtgaa
     3421 aactccgccc ggatcgctgc tgctgcgtac ccacacgtcg ccagtgcaga tccgcaccat
     3481 ggaaaaacag aagccgcccg tgcgcgttat cgttccgggt aaggtctatc gccgcgacaa
     3541 tcccgacgcc acgcactcct tcatgtttca ccagattgag ggcttggccg tcgacaccga
     3601 catcactttc tgcgatttca cgggaaccat cgaatatttc atcaaacagt attttggccc
     3661 gtcgatgaaa gcccggtttc gtcctagcta tttcccgttc accgagccca gcgtcgaatt
     3721 cgacgtctcg tgcattttct gcggcggcag cggcaccggc gcaagcggcg cgacctgcgg
     3781 caagtgcaaa ggcgccggct ggatcgagct ttttggcgcc ggtatggtcg atcccgctgt
     3841 ctat
//
LOCUS       DASTPM010000659         2831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1263853, whole genome shotgun sequence.
ACCESSION   DASTPM010000659 DASTPM010000000
VERSION     DASTPM010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263853"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..708)
                     /locus_tag="VFR42_11620"
     CDS             complement(178..708)
                     /locus_tag="VFR42_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369855.1"
                     /translation="MTLLILYLLASLDGLLCGCRAAMGRCPLIRLRPYYVVALLRGFF
                     AAQVASLIAIIALALVFLLTPHRGELRGDLELAAHRMLWTFLPYAATVLFSLALRLLP
                     STDIRSASSVFLLGPLTAIRPVLMTAGVLFGIYSAHLWETRLLGLIVLAFMLSVEIIL
                     NRLSSRRQSAEICSLV"
     gene            complement(705..1751)
                     /locus_tag="VFR42_11625"
     CDS             complement(705..1751)
                     /locus_tag="VFR42_11625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0369856.1"
                     /translation="MSTCYSPAEQLLLGCLLRLRSLYRCAIGIRYAVPVLQPFRPLNH
                     LTEDQPEITDLIVSHDPVTLERLPVRKESVLDAFASFQNRRAIAAVRAIPDRNGFLDS
                     VAVDPLLLTVHWEMQRLAEEFFHGQRVSELLGTVISFLRAQGVQGPLRVVDVGCGIIR
                     WLAARTALPSQGIELVGVDLNSTLIREAARLAAAENLPCRFLHGDAFSSAHAAHIYLS
                     TGFMHHFRAGALQQLLQRHDLADTAAFLHYDFRPWCLAPYGSWFFHALRMRTAIARHD
                     GVLSTVRAYTGDSLVRASRAALPSFSSGIYGANIWGTPLPRVFHTLLGLRRKLLPAFR
                     SFLGVRAVRLGEMS"
     gene            1819..2247
                     /locus_tag="VFR42_11630"
     CDS             1819..2247
                     /locus_tag="VFR42_11630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0369857.1"
                     /translation="MSEGTKSVKAAVKTLIAPMLSVRNGVKALEFYREAFGAEELFKI
                     ENGGSVVAQLAVEGAEFWVADESPEHLNFSPETLGGGTVRMVMIVSDPDAAFERAVKA
                     GAKVVVPVKNEYGWRLGRVVDPYGHHWEIGRPDGTIVTGE"
     gene            2497..2691
                     /locus_tag="VFR42_11635"
     CDS             2497..2691
                     /locus_tag="VFR42_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369858.1"
                     /translation="MDTMAQITILTAATVLAAAAAFAMAWAFLCGAFRLMQPAARPAR
                     PALELVQGTRAVVRGFALHR"
BASE COUNT          652 a          624 c         1064 g          491 t
ORIGIN      
        1 ccaggggcga gaggatcgat cggagcagag ccaagaacag gggtggcgac cgtttgtatt
       61 ccggcacggt cgaaattgtt gagcaggaag agtattggaa ggaaattaga aacctgccgc
      121 aaggccagcg aaaaaagtcg attcgagctc taggtcagta ctcgtcatga accaaggtca
      181 gacgagggaa caaatctcgg ccgactggcg gcgggaagag aggcgattga ggatgatttc
      241 gacggagagc atgaaggcga ggacgatcag gccgagcagg cgagtttccc agaggtgggc
      301 ggagtagatt ccgaagagaa cgccggcggt catgagtacc gggcggatgg cggtgagcgg
      361 gccgagcagg aagacgctgc tggcgctgcg aatgtcggtc gatggtagca ggcgcagagc
      421 gagcgaaaac aagacggttg cggcatacgg gagaaaggtc catagcatgc ggtgggcagc
      481 gagctccaga tcgccgcgga gttcgccgcg atgcggtgtg agcaggaaca ccagagccag
      541 cgcgatgatg gcaatcaggc tggcgacctg agcggcgaaa aagcctcgta aaagtgctac
      601 aacgtagtag ggacgaaggc ggatgagcgg gcagcggccc atggcggcgc ggcatccgca
      661 aagcagtccg tcaaggcttg ccagcagata gagaatcagc agggtcatga catttcgccc
      721 aatctgacgg cgcgcacacc aagaaaactt ctaaaagccg gcaagagctt gcggcgcagg
      781 cccaacaaag tgtggaagac acggggaagc ggcgtacccc agatgttggc tccgtaaatt
      841 cccgaagaaa aagatggaag tgcggcgcgc gatgcgcgaa cgagggagtc gccggtgtag
      901 gcgcgaaccg tggagagaac gccgtcgtgg cgggcgattg cggtgcgcat gcggagtgcg
      961 tgaaagaacc aggaaccata aggcgcgagg caccaggggc gaaagtcata gtgaaggaag
     1021 gctgcggtgt ctgccagatc gtgacgctgc aggagttgct ggagagcgcc agcgcgaaag
     1081 tggtgcatga agccggtgga aagatagatg tgcgcggcat gagcggagga aaacgcgtcg
     1141 ccgtgcagaa aacggcaggg gagattttct gcagcggcaa gacgagccgc ttcgcggatg
     1201 agcgtggaat tcaggtctac gccgacgagt tcgattcctt gcgacggaag tgcagtgcgc
     1261 gcggccagcc accgaatgat gccgcagccg acatcgacca cacgtaaggg accttgaact
     1321 ccttgcgcac ggagaaaaga gattaccgtg ccaagaagtt cggagacgcg ctgaccgtgg
     1381 aagaattctt cggcgaggcg ctgcatttcc cagtggacgg tgagtagaag gggatcgaca
     1441 gcgacggaat ccaggaaacc gttgcggtct gggattgcac ggacggccgc gatggcccgg
     1501 cgattttgga agctcgcgaa tgcatcgaga acggactctt tgcggacagg aagacgttcc
     1561 agagtgaccg ggtcgtgcga gacgatcagg tccgtgatct caggctgatc ctctgttaga
     1621 tgatttaaag ggcggaaggg ctgcaacaca ggcacagcgt agcggatgcc gatagcgcag
     1681 cgatagagcg aacgtagtcg aaggagacaa ccgagcaaca gctgctcggc gggtgaatag
     1741 catgttgaca caggcggccg cagggcggtg gtaaactccc gcgaatctgc aggctgaatc
     1801 tgcacgctgg aggtacaaat gagcgaaggg acgaagagcg tcaaggcggc ggtgaaaact
     1861 ttgatagcgc cgatgctgtc ggtgcggaat ggggtgaagg cgctggagtt ttatcgggag
     1921 gcgtttgggg cggaggagtt gttcaagatc gagaatggcg gatcggtggt ggcgcagctg
     1981 gcggtggagg gagcggagtt ctgggtggcg gatgagtcgc cggaacatct gaatttcagt
     2041 ccggagacgc tcggtggcgg gacggtgcgg atggtgatga ttgtgagcga tccggatgcc
     2101 gcgtttgagc gagcggtgaa ggcgggagcg aaagtggtag tgccggtaaa gaatgagtat
     2161 ggttggcggc tggggcgggt ggtggatccg tatgggcatc attgggagat tgggcggccg
     2221 gacggaacaa tagtgactgg tgagtgattg gaagcaagaa actgtccctg gccggcgcgg
     2281 gattcctagt cctatgggtg tgcacgagtt cgatggcctg ctgacgcttc gatttcccct
     2341 caaaaagcca tttggtgtgg tgagtctggc gtagtacaca ccgtgtgtcg gcgtacacga
     2401 cgttcttctt cgtgaaagca tgtaacatcc ttaatttcaa ttagttagct gtggttgtcg
     2461 gagtgcgtag gtgtgtatgt aagacgggag gcagacatgg acacgatggc ccagataacg
     2521 attttgacgg cggcgacggt tttggcggct gctgcggcgt ttgccatggc ctgggcgttc
     2581 ctctgcgggg cgttccggtt gatgcagccg gcggcgcggc cggcgaggcc agcgttggag
     2641 ttggtgcagg ggactcgggc ggtcgtgagg ggattcgcgt tgcatcggta gacaaggatg
     2701 atggaggcca cctgaaggtg gccgctacaa gaagcggaag aaagggagag cggcgatggt
     2761 ggcactgaag tggttgctgg tgattctggg gctggggttg tttggaagcg cgggcgcgct
     2821 ggttgtgtac g
//
LOCUS       DASTPM010000660         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1436748, whole genome shotgun sequence.
ACCESSION   DASTPM010000660 DASTPM010000000
VERSION     DASTPM010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436748"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /gene="secF"
                     /locus_tag="VFR42_11640"
     CDS             <1..847
                     /gene="secF"
                     /locus_tag="VFR42_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU0369859.1"
                     /translation="SSVAAYLTQKDPLNLSAAAGDRYQQLARQILDYRDRALNGLVTN
                     FDDLTKAPSVTPAVVASLRDGFALGPFAVRNAEIVGAKVGAELRRQAIYVTLYALGGM
                     LLYIAFRFEWVFRAAAVLAVFHDVLITLGFFSLLHFEISLTVIAALLTLVGYSMNDTI
                     VIFDRIRENNRLLRKEDFKDLVNKSINQTLSRTILTSGLTFLTVLVLFLMGGQVLRAF
                     SFALVVGIVVGTYSSFGIAAPLVVWWHRWKGHGAAAVGTGPAAGNKPRNGETSTARLA
                     PAGRR"
     gene            853..1587
                     /locus_tag="VFR42_11645"
     CDS             853..1587
                     /locus_tag="VFR42_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU0369860.1"
                     /translation="MFEEMVVSSPKGKKTNKSWTVIVSGIVQATFLAVLILIPLIYTE
                     ALPKASLATLLVAPPPPPPPPPPPAATQIVKVKPQVHLMDAGKLVAPKVIPKEVKIIK
                     EEAEQPDAMGAIGGVPGGVPGGQMGGVIGGVIGGVGGAPPPPKPAQQRIRQGGAVQAA
                     MLVNRVQPQYPPLARQTRISGTVRLHAIISKDGSVQQLEVLSGHPLLVQAALDAVRQW
                     KYRPTTLNGEPVEVDTTIDVIFSLNQ"
     gene            1753..>2169
                     /locus_tag="VFR42_11650"
     CDS             1753..>2169
                     /locus_tag="VFR42_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369861.1"
                     /translation="MVATLYVYAAMFLQSTNGSQWDLRTMWGQMSLLPRAVVITLFIL
                     SIYSFGVMIDRALMYSAARKQSRIFVQQVAGALREGKIDEAIKVAERNKKSHLAKVVT
                     AGLQEFRAHGDSADIPGEQIEASKRALERAEAIVHAE"
BASE COUNT          434 a          623 c          656 g          456 t
ORIGIN      
        1 ttcctcggtc gctgcttacc tgacccaaaa agacccgctg aatctttccg cggcggccgg
       61 cgatcgctat caacagctcg caagacagat tttggattat cgcgaccggg cgttaaatgg
      121 actggttacg aattttgacg atctgacgaa ggcgcctagc gtcacgccgg ccgtggttgc
      181 ttcattgaga gatggtttcg cgcttgggcc ttttgctgtg cgcaacgcgg aaatcgtcgg
      241 cgcgaaagtg ggcgcggagc tacgccgcca ggccatctac gttaccctct atgctttggg
      301 cggcatgctg ttatatattg ccttccgatt cgagtgggtc tttcgggcag cggctgtcct
      361 ggcggttttc cacgacgttt tgatcacgct gggattcttc tctcttttgc actttgaaat
      421 ttcgctgacg gtcatcgcgg cactgctcac tctggtcgga tattcaatga atgacaccat
      481 tgtgattttc gaccgcattc gcgagaacaa tcgtttgctg cgcaaggaag actttaagga
      541 ccttgtcaac aaatccatca accaaacgtt gtcgcgaacg atactcacca gcggtctgac
      601 ttttctcacc gtgctcgtac ttttcctcat gggcggccag gtgcttcgcg cgttctcctt
      661 tgccctggtg gtggggattg tggtaggaac gtactccagt tttggcatcg ctgcgccgct
      721 cgtggtttgg tggcaccgtt ggaaaggcca tggagcggca gcagtaggaa ccggacccgc
      781 agccggcaat aaaccccgga atggtgagac gagcacagcg cgtcttgctc cagccgggag
      841 gaggtaggga ccatgttcga ggaaatggta gtttcgagtc cgaagggaaa gaagaccaac
      901 aagtcctgga ccgtgatcgt gtccggaatt gtgcaggcca cctttctcgc ggtgctgatc
      961 cttattccgc tgatctacac ggaagcgctg cccaaggcaa gcctggcgac gctgctggtc
     1021 gcaccgccac cgccgccacc gccaccgcca ccgcccgcgg cgacgcagat cgtcaaggtg
     1081 aagccgcaag tccatttgat ggatgccggc aaactggtgg cgccgaaagt catccccaag
     1141 gaagtgaaga ttattaagga agaagccgag cagcccgacg ccatgggcgc catcggtgga
     1201 gttcccggcg gcgttccggg cgggcaaatg ggcggcgtga tcggcggtgt gattggtgga
     1261 gtgggtggtg caccacctcc tccgaagccg gcgcaacagc gcatccgcca gggcggcgcg
     1321 gtgcaggccg ctatgctcgt gaatcgtgtg cagccgcagt atccgccgct ggcgcgccaa
     1381 acgcgcatct ccggcaccgt gcgcttgcac gcgatcattt ccaaagacgg ttctgtgcag
     1441 caactcgaag tgctgtctgg gcatcctctt ttggtacagg cagctcttga tgcagtgcgt
     1501 cagtggaagt accgcccgac cactctcaac ggagaaccgg tcgaagtcga taccacgatt
     1561 gacgtgatct tttcgctgaa ccagtagttc gagcagtaaa cgaggcgcca cgcgggtcgt
     1621 gggacgtgca gggcgcttcg cgcagcacag atttggcagc aagcttcgtt ggttggcggc
     1681 gcgaagagca acgcaagcca tacggaacaa atcaaggacg gccgttcagc ggccagtcta
     1741 aggagcaaac gaatggtagc cactttgtac gtttatgccg ccatgttcct gcagtcgacc
     1801 aacggctcgc aatgggacct gcggaccatg tggggacaga tgagccttct gccccgggcg
     1861 gtcgtaatca ccctgttcat cctttccatt tactcgttcg gcgtgatgat tgatcgcgcg
     1921 ctgatgtata gcgccgcgcg caaacagtca cgcattttcg tgcagcaggt tgccggcgcc
     1981 ttgcgcgaag gcaagatcga cgaagccatc aaagttgccg agcgcaacaa gaagagccac
     2041 ctggcgaagg tcgtcaccgc cggcctgcag gaattccgcg cgcacggtga ttccgccgac
     2101 atcccgggcg agcagatcga agcctccaag cgtgctcttg agcgcgccga agccatcgtt
     2161 catgccgag
//
LOCUS       DASTPM010000661         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_656874, whole genome shotgun sequence.
ACCESSION   DASTPM010000661 DASTPM010000000
VERSION     DASTPM010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656874"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1127)
                     /locus_tag="VFR42_11655"
     CDS             complement(141..1127)
                     /locus_tag="VFR42_11655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU0369862.1"
                     /translation="MRKSHSQILAACLILTFGASTASAQIGDALRRAQNNAQKAKKAA
                     DIYTAWTAEQEQAIGEASAARLIHIFGTYENAEMVKYVNLVGNTVARQGSRTVPYRFA
                     VLDTEVITALSLPGGYIFITRGALANLHNEAELAGTLAHEIAHVDRRHLEKEIRSKKT
                     SQFAKEETVTRVPQGAELVNLAGDVVKNALTLQVSRDKESEADKVGMEFAAKAGYDPA
                     GLRNFLQTLAEAPSTDQSRRQLSLWGSTHPPFSARVSELNSLLASYPAGGQQLQERYS
                     WYVNPVAFSKSSSAGATSGGSSELDGVVSQGVVVLTGGKLAEGTRVKVRIQH"
     gene            complement(1137..1622)
                     /locus_tag="VFR42_11660"
     CDS             complement(1137..1622)
                     /locus_tag="VFR42_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369863.1"
                     /translation="MKTWIMQSVVSALLFFLSCAGWGFSPPTQSPQQQQQQGSQQSQT
                     DAKQKKGQTAGKDTPPPGGTSNANAPANGSGAEKPAPLFGGSLNIKTSRQTKDTATLG
                     FNGVDDNGQVQKTFLASTATGDDTAKVQKLATYAVSTAELDEFIQQGGLNPAAAPKKP
                     N"
     gene            complement(1637..3580)
                     /locus_tag="VFR42_11665"
     CDS             complement(1637..3580)
                     /locus_tag="VFR42_11665"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0369864.1"
                     /translation="MLLLAKTQTGEWLENGTLDARTRWTAQRAKADPRIVIIDIDNAS
                     LASLQEKLGRWPWTRRVWTEVIRYVSRGKPSVIVMDAVLSGVESESVDAELAQVAQRS
                     GRLVLGFSFLSTQVERVNTDSQQAQLVVLEEQASRGGLGVPIPSQAFEPNLPQEKLAR
                     AAASLGCVNSSPDRDGTVRRVPVQFEYRGKHYGSLAARGADLASPESQAISWHKKSGI
                     FDASYIERNGIRTPVDEQGRILVLWHGGSLVYPRLPIWKVVCSIYPEQCPAEVQKYPP
                     EFFRDKIVLIGASAAGSYEARPMPFDEQAPGFLAHAAAIDNLLHGEALRLAPEWLLFG
                     AVLAMACFGGLLQYRLRSISTGGAINIAALVAYVAIALVAFRGLHLAVPVVAPCLALV
                     VSYGSTGAARYVTTGRELRRTRGVLDRYVAPQLVNYVMSNLDSFRLNGDKRELTILIS
                     DVRNFTTMTEQSEPMQLISLLDDYLAAMTDIIFKHNGIVDKFIGDGILAYWGAFTPGE
                     NHAEEAAQAALEMIEKLKQLNEQWAKQGQGPIAIGIGVNTGSVIFGNIGRGKKIEFTV
                     IGDAVNLAARLEGLNKEFHTSIVISETTRRMLGEAGEVRSLGGVKVKGKTVETLVYEL
                     VGFKRMAPAVAPIENKIAIETVE"
BASE COUNT          788 a         1164 c         1258 g          923 t
ORIGIN      
        1 catgaaggaa tgatgatacg cggtgaggta ccgtggcgca atccaaccag gggagagcga
       61 gaaccagacc tcttgtacta gcggttgtgg aacttagagt ttctcggatg gctgccctgc
      121 gtcttgcaag cctctgggcc ctagtgctgg atgcggactt tgacgcgcgt gccttcggcc
      181 aattttccgc cagtcagcac gaccacgccc tggcttacga caccgtcgag ttccgaagag
      241 ccgcccgacg ttgcgcccgc ggaactgctc tttgaaaagg cgaccgggtt gacataccag
      301 ctgtagcgct cctgaagctg ctggccgccg gcggggtagc ttgcgagcag gctattcaat
      361 tctgaaacgc gcgcgctaaa cggcggatgc gtgcttcccc acaagctaag ttggcggcgg
      421 ctctggtcgg tgctgggcgc ctcggcgagc gtttggagga aattgcggag tccggccgga
      481 tcgtagcctg cttttgccgc gaactccatt ccgactttgt cggcttcgct ctctttgtcg
      541 cgactaacct gcagggttag cgcatttttg acgacatcgc cggctaaatt cacaagctcg
      601 gcgccctgcg gcacgcgcgt taccgtttct tccttggcaa actgacttgt tttcttggag
      661 cgaatctctt tttctaggtg ccggcggtcg acgtgggcta tttcgtgtgc gagagttccg
      721 gcgagttccg cttcgttgtg caggttggcc aacgcacctc gcgttatgaa gatgtatccg
      781 ccaggaagcg agagcgccgt gatcacttcg gtatccagaa ccgcgaaacg atagggaacc
      841 gttcggctgc cctgccgcgc gacagtgttg cctaccaaat tcacgtattt aaccatctcg
      901 gcgttttcat aagtgccgaa aatgtggatc agcctggcgg ctgaggcttc cccgatggcc
      961 tgttcctgtt cggcggtcca tgcggtgtag atgtccgccg ccttcttggc cttctgcgcg
     1021 ttgttctgcg cccttctgag cgcatcccca atctgggcgc tcgctgtgga tgcaccaaac
     1081 gtcagaatga gacaggctgc aagaatttga gagtgtgatt ttcgcattgt gcctcctcag
     1141 ttgggcttct taggagcagc ggccggattc agtccgccct gctgaatgaa ctcatcgagc
     1201 tccgcagtcg aaaccgcata cgtcgcaagc ttttgaactt ttgctgtgtc atcgccggtc
     1261 gcggtggacg ccagaaaggt cttttgcacc tgtccgttgt cgtcgacccc gttgaatccg
     1321 agagttgccg tgtccttggt ttgtcgcgaa gtcttgatgt tgagcgaacc gccaaagagg
     1381 ggcgcgggct tttcggcgcc ggatccgtta gcgggtgcat tggcgttgga cgttcctccc
     1441 ggcgggggcg tgtccttgcc tgcagtttgt cccttctttt gcttcgcatc cgtttggctt
     1501 tgttgcgagc cttgttgctg ttgttgctgt ggcgattgag tcggcgggga aaagccccag
     1561 cccgcgcagc tcagaaagaa gagcaaagcg ctgacgaccg actgcataat ccaagttttc
     1621 atgctgtctc ccgagttcat tccacggtct caatggcgat cttgttttcg atgggcgcta
     1681 cggccggcgc catccgcttg aacccaacaa gctcgtaaac cagcgtttca accgtctttc
     1741 ctttgacctt cacgccgcca agagaacgca cctctcccgc ttcgccgagc atgcggcggg
     1801 ttgtttcgct gatgacaatc gaggtgtgaa attctttgtt tagtccctcg agccgtgcgg
     1861 cgagattgac ggcgtcgccg atgaccgtga attcgatctt cttgccgcgg ccgatattcc
     1921 cgaagatgac cgagcctgtg ttcacgccga tcccgatggc gattggtcct tggccctgct
     1981 tggcccactg ttcattgagc tgttttaatt tttcgatcat ttccagcgcg gcctgggctg
     2041 cttcctccgc gtgattctcg ccgggcgtga aggctcccca ataagccagg atgccgtcgc
     2101 cgataaattt gtcgacgatg ccgttgtgct taaaaatgat atcggtcatc gccgcgaggt
     2161 agtcatcgag cagcgagatc aattgcatgg gttcggattg ctcggtcatg gtggtgaaat
     2221 tgcggacatc agaaatgaga atggtcaact cacgcttgtc gccgttcagg cggaagctgt
     2281 ccaggttgga cattacgtaa tttacgagct gcggcgcgac gtagcggtcg agcacgccac
     2341 gggtgcggcg cagctcgcgt ccggtggtga cgtatcgcgc ggctcctgtc gagccgtagg
     2401 acacgaccag ggcaaggcac ggggcgacaa ccggaactgc caagtgcaaa ccgcgaaacg
     2461 caaccagggc gatcgcgacg taggcaacca aggcggcgat gttaatcgcg ccgcccgtgc
     2521 tgatggatcg cagacggtac tgaagcaggc ctccgaaaca ggccattgcg aggacggcgc
     2581 cgaacaagag ccattcgggt gccaagcgaa gcgcttcgcc gtgcagcaga ttgtcgatgg
     2641 cagcggcgtg cgccaggaag cccggcgctt gctcatcgaa gggcatgggg cgcgcttcat
     2701 agctgccggc ggcgctggct ccgatgagaa cgattttgtc ccgaaaaaat tcgggaggat
     2761 atttctgcac ttcggccggg cattgctcgg gataaatgga acagaccact ttccagatcg
     2821 gcaggcgcgg atagactagc gagccgccat gccacagcac gaggatgcgg ccttgttcgt
     2881 cgacgggcgt gcggattcca ttccgctcga tgtagctggc gtcgaaaata ccgctctttt
     2941 tgtgccatga aatggcctgc gactcaggac tcgccagatc ggcgccgcgt gcagcaagcg
     3001 agccgtagtg cttgcctcga tattcaaatt gcaccggaac gcgccgaacg gttccgtccc
     3061 gatcgggact cgagttgacg catcccaatg aagcggcggc gcgcgcgagt ttttcctgcg
     3121 ggaggttggg ctcgaatgcc tgggagggta tcgggacacc aaggccgccg cgactcgcct
     3181 gttcttccag caccaccagc tgtgcttgct gcgaatccgt gttgactcgc tcgacttgcg
     3241 tggaaagaaa ggaaaagcca agaacgagcc tgcccgagcg ctgcgcaacc tgggcaagtt
     3301 cagcatcgac ggattcgctc tccacaccgc tcaaaacagc gtccattacg atgacgctgg
     3361 gttttccgcg actgacgtag cgaattactt cagtccagac gcggcgcgtc cagggccagc
     3421 gaccgagctt ttcctgcagg cttgccaggc tggcgttgtc aatatcgatg atgacgatgc
     3481 gcggatcggc tttggctcgc tgcgcagtcc agcgggtgcg cgcatccagg gttccgtttt
     3541 ccagccattc gcccgtttgc gtcttagcaa gcaaaagcat caaggccgca gaaacggcga
     3601 tacatgcgaa cgtgcctcgg cgaaacgcgc gctgggaatg cggatcggtg gtcatccgtt
     3661 tagctgcttg cctctggccg tttaccgaag agagttgtgc ggaattttcc tgttgcgatg
     3721 ttaggttgta gccgcagagc aagtcaatga attgaggttt actgtttgga ccatgcgtcc
     3781 ggtgcagagc cagagcgcca agcctgagcg ccaggcggtc cttcgctccg cccggtccgg
     3841 ccgagcggga gcccgcgcca gggaatgatt attttagagg gcagatgtcg atttctgtcc
     3901 ggctcaacgt cgctccaata aggcactcgc tgccgagagg tagcggcgcc ggccggatga
     3961 ggcactccgc gcgaacttcg tcagggacaa ccggcgaata aagtggaata cagcgttgta
     4021 tagactagca aggcattttc tgacagcaaa ggagccaaaa tgcaatttct actactgatt
     4081 tacgagaatg agcagcggtt cagccagggg ttcgaccaga aggagatgga cga
//
LOCUS       DASTPM010000662         3840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_52679, whole genome shotgun sequence.
ACCESSION   DASTPM010000662 DASTPM010000000
VERSION     DASTPM010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52679"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..2272
                     /gene="tkt"
                     /locus_tag="VFR42_11670"
     CDS             203..2272
                     /gene="tkt"
                     /locus_tag="VFR42_11670"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0369865.1"
                     /translation="MSQQALVTQQIAPDQLCINTIRTLSIDAVQQANSGHPGAPMGLA
                     PVVYELWQKYLKYDPANPIWPNRDRFVLSAGHASMLLYSTLHLAGVRAVNEKGEVLDS
                     LAVPMEEIKRFRQLDSKTAGHPESHLTTGVETTTGPLGQGAGNSVGMAIAGKWLAANF
                     NKPNFELFNFKVYALCGDGDLMEGVAAEAASLAGHLRLDNLCWIYDNNHVTLDGPANW
                     SFSEDVMTRFVGYGWNVTRVADANDLMMLGRGYETFLKTSGKPTLVVVDSHIGYGSPH
                     KQDTYEAHGEPLGDAEVKLVKKNYAWPEDSKFLVPDGVYDNFKNGIGKRGAEAHAQWV
                     KLWDDYAKQYPEQAKQLELMQKRALPDGWDKSLPTFPADAKGMASRESSGKTVNALAK
                     NIPWLVGGSADLAKSNKTNLDGAGEYLPGEYAGRNVHYGVREHAMGAAMSGIAECRLR
                     PFGGTFFNFCDYMKPAVRLAALMELPVIYVFTHDSIGLGEDGPTHQPIEQLASLRAMP
                     NILVLRPGDANEVVEAWKLAVRQSHRPTALVLSRQVMPTLDRAKFAPASGVAKGAYVL
                     SDAPGGKPDVILIGTGSEVGLCVATQEKLAAEGIKARVVSIPSWELFEEQDAAYKESV
                     LPAAVTARVSVEMASTFGWERYTGPKGKIVGMRTFGASAPLKDLLRKFGFTVENVVAV
                     AKQSLGK"
     gene            2280..2726
                     /gene="rpiB"
                     /locus_tag="VFR42_11675"
     CDS             2280..2726
                     /gene="rpiB"
                     /locus_tag="VFR42_11675"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEU0369866.1"
                     /translation="MKLVIGSDHAGFELKNAMGDVLRGLGHAVLDVGAFNTNPSDYPD
                     FAEAVGRAVLDGRVERGVLICGSGVGASVAANKLVGIRAAVCHDTYSSHQGVEHDDMN
                     VLVLGSRVIGVKLAEDLVKAFVGANFTNEERHKRRLAKVKALEDKR"
     gene            2837..>3840
                     /gene="tal"
                     /locus_tag="VFR42_11680"
     CDS             2837..>3840
                     /gene="tal"
                     /locus_tag="VFR42_11680"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEU0369867.1"
                     /translation="MTTTTTLPNLAGVKGENPLKGLLAYGQSPWLDYIRRNILLNGDL
                     KKMIDNDGLRGMTSNPAIFEKAITAGDDYNDIIKAADAKSSTAMALYEKIAIRDVQEA
                     TDIFGPVHKETKGRDGYVSLEVSPNLAFDTQATIEEARRLWKTVNRPNVMIKIPATPQ
                     GLSAIRQALEDGINVNITLLFAQSAYEQVAEAYFAAFEARAAKGQDISHIASVASFFV
                     SRIDSLVDSTIDAKQKTETESAKKALLESLRGKVAIANARRTYAKYQELIATPRWKAL
                     AAKGAQTQRLLWASTSTKNPKYRDVLYVEELIGADTVDTIPPATFDAFRDHGKLRKSL
                     T"
BASE COUNT          870 a         1115 c         1114 g          741 t
ORIGIN      
        1 agccactact acggggccgc caactgatcc atgggccggg cgcatttccc tcgagtaaac
       61 gaactttaac ctcttggtgg ggcggcacaa ctcggcattc cgccgccact tgacgtagaa
      121 tctccctagc cggtgcgtac ctccggtgca tctaaatcct gacttggatt gcggggaccc
      181 tcctgcccgc acaggagttt ttgtgagcca gcaagcattg gtgacccaac agattgcgcc
      241 agaccagctg tgcatcaaca ccatccggac tctttcgatt gacgcggtgc agcaggcaaa
      301 ttccgggcat ccgggcgcac cgatgggcct agcaccggtc gtttacgaac tgtggcaaaa
      361 atatttgaag tacgatccgg cgaatcccat ttggccgaac cgcgaccgtt ttgtgctttc
      421 cgcgggccac gcttcgatgt tgttatattc cacgctgcat ctcgccggcg tgcgcgccgt
      481 aaacgaaaaa ggcgaagtgc tcgactcact cgccgttcca atggaagaga tcaagcgctt
      541 ccggcagctg gacagcaaaa ccgccggcca cccggagtcg catctcacaa ccggcgtgga
      601 aacaacgacg ggcccgcttg gccaaggcgc aggcaacagt gtgggcatgg caattgccgg
      661 aaagtggctc gcggcaaact tcaacaagcc caacttcgag cttttcaact tcaaggtgta
      721 cgcgctgtgc ggcgatggcg acttgatgga aggcgtcgcc gccgaagctg cttcgctcgc
      781 aggacacctg cggctcgata atctttgctg gatctacgac aacaatcacg taacgctcga
      841 cggcccggcg aactggtcgt tcagcgaaga tgtgatgacg cgtttcgtcg ggtacggctg
      901 gaacgtcacg cgcgtcgccg acgccaacga cttaatgatg ctgggccgcg gctacgaaac
      961 ttttctcaag acgtccggca agccgacctt ggtcgttgtg gacagtcaca tcggctatgg
     1021 ctcgccgcac aagcaggaca cttacgaagc gcacggcgaa cctctcggtg acgccgaagt
     1081 caagctggtc aaaaagaatt acgcatggcc ggaagattcg aaattcctgg tgcccgacgg
     1141 tgtctacgac aatttcaaga acggcatcgg caaacgaggc gcggaagctc atgcgcaatg
     1201 ggtgaagctc tgggacgact acgccaaaca atatcccgag caggcgaaac agcttgaatt
     1261 gatgcagaag cgcgcgctgc cggacggctg ggacaaaagt cttccgacct tccctgccga
     1321 tgcgaagggt atggcttcgc gcgaaagctc cggcaaaacg gtcaacgcgc tggccaaaaa
     1381 tattccgtgg ttggtcggcg gttcggcgga cctggcgaaa tccaacaaga cgaatctcga
     1441 tggcgccggc gagtatctgc ccggtgagta cgccggccga aacgtccact atggcgtgcg
     1501 cgaacatgcc atgggtgcgg caatgagcgg catcgcggaa tgccgtctgc gtcccttcgg
     1561 tggaacgttt tttaatttct gcgattacat gaaaccggcc gtgcgcctcg ccgcattgat
     1621 ggagctgccg gttatttacg tgtttacgca cgattccatc ggcctcggtg aagatggtcc
     1681 tacgcaccag cccatcgagc agcttgcatc cctgcgcgcg atgccgaaca ttttggtgct
     1741 gcgtcccggc gatgctaacg aagttgtcga agcctggaag ctcgccgtgc ggcagagcca
     1801 ccgtccgacc gcgctggtcc tttcgcgcca ggtcatgccg acgctcgatc gcgccaaatt
     1861 tgcccccgca tcgggggttg cgaagggagc atacgtcctg agcgatgcgc cgggggggaa
     1921 accagacgtg attttgatcg gcactggcag cgaagtcggc ctatgcgtcg cgacacaaga
     1981 aaagcttgcg gcggaaggca tcaaggcgcg cgtcgtcagc attccatcct gggaactttt
     2041 tgaagagcaa gacgccgctt acaaggaaag tgttctgcca gccgcggtca cggctcgcgt
     2101 ctccgtcgaa atggcttcta cttttggatg ggagcgttat accggcccta aaggcaagat
     2161 cgtggggatg cgcacatttg gcgcgtcggc tccgttgaaa gaccttctga ggaaattcgg
     2221 cttcacggtc gaaaacgtag ttgccgtcgc caaacaatct cttggaaagt gaaccagcga
     2281 tgaaactcgt gatcggatcg gaccacgcgg gattcgaact gaaaaacgcc atgggcgacg
     2341 tcctgcgcgg cctgggacac gcggttctgg atgtcggagc attcaacacc aatccatccg
     2401 actatcccga tttcgccgaa gctgtcggcc gtgccgttct cgatggacga gtcgagcgcg
     2461 gcgtgctgat ttgcggcagc ggcgtgggcg ccagtgtcgc tgccaacaag ctggtcggta
     2521 ttcgcgctgc cgtctgccac gacacctact cctcgcatca gggcgtcgag cacgacgaca
     2581 tgaacgtgct ggttctgggc tcgcgagtga ttggcgtgaa gctggccgaa gatctggtca
     2641 aagcatttgt gggcgccaat ttcaccaatg aggagcggca caaacggcgc ctggccaagg
     2701 tcaaagcact cgaagacaag cgctagttta gggaattcca acgtccgccg gagtcacatc
     2761 cgattaaccc tcgtcgctct cgacgggcgt acaatagaaa tagcgagcaa cgaatcgcga
     2821 aagtttagga ggacctatga ctacgacaac gacacttccc aatttggcgg gagtgaaggg
     2881 tgaaaatccc ctcaaaggct tgctagccta cggccaatcg ccctggctcg attacattag
     2941 gcgcaatatt ttgctgaacg gcgatttaaa gaaaatgatc gataacgacg gcctgcgcgg
     3001 tatgacgtcg aatcccgcta tcttcgagaa ggcgatcacc gcaggtgacg actataacga
     3061 catcatcaag gcggcggatg ccaagtcctc tacggccatg gcgctttacg aaaaaatcgc
     3121 catccgcgac gttcaggagg caaccgacat tttcgggccc gtgcataaag aaaccaaggg
     3181 ccgcgacggc tatgtcagcc tcgaggtctc cccgaacttg gcattcgata cacaggctac
     3241 tatcgaggaa gcgcgccggc tttggaaaac cgtcaatcgt cccaatgtca tgatcaaaat
     3301 tcccgcgact ccgcagggtc tttcggcgat tcgccaggcg ctcgaagacg gcatcaacgt
     3361 caacatcaca ctgctctttg cgcagtctgc ctacgaacag gtggcggaag cgtatttcgc
     3421 ggccttcgaa gcgcgcgccg cgaagggcca ggacatttcc cacatcgcca gcgtagccag
     3481 cttttttgtc agccgcattg attcgttggt ggatagcact attgacgcga agcagaaaac
     3541 cgaaacagaa tcggccaaaa aagcgctgct ggaatcgctg cggggcaaag ttgccattgc
     3601 caatgcgcgc cgcacttacg ccaagtacca agaacttatc gcgacgcctc gctggaaagc
     3661 acttgccgcc aaaggcgcgc agacgcagcg gttgctctgg gccagcacca gcaccaagaa
     3721 tcccaagtat cgcgacgtgc tgtacgtgga agagctgatc ggcgcggaca ctgtggatac
     3781 cattccgccg gcaaccttcg atgcgttccg cgatcacggc aagttgcgca agagccttac
//
LOCUS       DASTPM010000663         3994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_917789, whole genome shotgun sequence.
ACCESSION   DASTPM010000663 DASTPM010000000
VERSION     DASTPM010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917789"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFR42_11685"
     CDS             <1..319
                     /locus_tag="VFR42_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0369868.1"
                     /translation="TRDEVLKWMREQDLNLDQLDTIVLLDKAAQFSGTVPVARLLLAE
                     PEQTLAELKMEPLLSLPPDASDKEVFEIFDKYNLRMLTIVDEESRPMGTVTVDDVVSK
                     LLK"
     gene            345..1712
                     /locus_tag="VFR42_11690"
     CDS             345..1712
                     /locus_tag="VFR42_11690"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU0369869.1"
                     /translation="MWNRENIAAQWAGWAERVERAMAVVRPYRRRIALFLAVVGPGLI
                     VSNVDNDAGGIYTYAQSGAKYGNTVLWSLIPMTIALYVSLEMCSRMGAVTGKGLSDLI
                     REEFGFRVTFVLMALVLIVNLGNVMAEFTGVFWASEVFGISRFISVPLAGLLVWALVV
                     AGTAKVVERIFLTVCVVYFSYVISAFLAKPEWLISLLHLLPPQLMHYIPTVRPWIQVP
                     PFNASYMVILAGLVGTTIAPWQHFYLQAAVVEKRVGPRQYAATRNDVLVGSISCMVIV
                     FFIIVCTSATLFNNGVHEIDNAGTAALALKPLAGKWAAALFALGLLNASLFAASILPL
                     STSYVVCEALGFESGIDRKFGEAKIFYALYTALIALGVVFVLFLGRFAQTILLWSQVL
                     NGFLLPVVMIFVLILVNRRDLMGEKTNPKGFNWVAWICTVAMIVLTLIVAYSSIRELF
                     GHAST"
     gene            1849..2694
                     /locus_tag="VFR42_11695"
     CDS             1849..2694
                     /locus_tag="VFR42_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU0369870.1"
                     /translation="MTDPKSTKTLHVAASHPLGTVTAEEAFLLEKLNLSRLPRHVAVI
                     MDGNGRWAQRRHLPRIAGHRKGTETARTTIETCARLKIEALTLYAFSVENWRRPKTEI
                     DFLMQLLREYIRQEMPLIQRNDIRMRFLGRADELPEEVQRDTREATEATAGNSGMVLC
                     IALNYGGRAEILDAANALLAERRAAASTAKLTDADIERHLYTSGLPDPDLMIRTSGEM
                     RVSNFLLWQIAYAEIFVTETLWPDFNRARLLESFVEFQKRDRRYGGIGDGDESERVAS
                     ELGAK"
     gene            2804..3685
                     /locus_tag="VFR42_11700"
     CDS             2804..3685
                     /locus_tag="VFR42_11700"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0369871.1"
                     /translation="MTWKRVATAVVLIPAVIGLVLGAPTAWVAVALAGVMLLSLFEFF
                     ALGDAIGHRAYKFWTAVCACLLVFSQYLAAMDYAHSLGDGLSLHRIIGRFAVRGPTVA
                     DVLFIFLMGVAVLTLATRRPLVEALPAAGISSSALLLVALPLTYSIRLHGVSLAGKWL
                     LLFALVITWIGDTAAYFVGRAIGRHPFAPHLSPKKTWEGAVASFAGSLLVAWAMARWG
                     RVNVATVHVLGMAAAGNVAGQMGDLLESAYKRSAGVKDSGALLPGHGGILDRIDALIL
                     TFPVVWYYWLWIYSPRV"
     gene            3762..>3994
                     /locus_tag="VFR42_11705"
     CDS             3762..>3994
                     /locus_tag="VFR42_11705"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369872.1"
                     /translation="MKQLAILGSTGSIGRQTLAVVEALGERFGVVALAAGNNLDELAG
                     QIERYQPELVSVADAAKADALARRLREKGIAPLP"
BASE COUNT          734 a          947 c         1379 g          934 t
ORIGIN      
        1 gacgcgggat gaagtgttga agtggatgcg ggaacaggac ttgaacctgg atcagttgga
       61 tacgatcgtg ctgctggaca aggcggcgca gttttcggga acggtgccgg tggcgaggct
      121 gctgctggcg gagccggagc agacgttggc ggagttgaag atggagccgt tgctttcttt
      181 gccgccggac gcgagcgaca aggaggtgtt tgagattttt gacaagtaca atttgcggat
      241 gctgacgatt gtggatgagg aaagccggcc gatgggtacg gtgacggtgg atgacgtggt
      301 gtcgaagttg ttgaagtaga tagacgaagg acaacgatag cggaatgtgg aacagggaga
      361 atatcgcggc gcagtgggcg ggatgggcgg agcgcgtgga gcgcgcgatg gcggtggtgc
      421 gtccataccg gcggcggatt gcgctgtttc tggccgtggt ggggccgggc ctgatcgtat
      481 cgaacgtgga caacgatgcg ggcggaattt atacatatgc gcagtcggga gcaaagtacg
      541 ggaatacggt gctatggtcg ctgattccga tgaccatcgc gctgtatgtg tcgctggaaa
      601 tgtgctcgcg catgggagcg gttactggca aagggctgtc ggatttgatc cgcgaagagt
      661 ttgggtttcg cgtgacgttt gtgctgatgg cgctggtgct gatcgtgaat ctgggcaacg
      721 tgatggcgga attcacgggc gtgttctggg cgagtgaggt cttcggaatc agccgattta
      781 tttcggtgcc gctggcggga ttgctggtat gggcgctagt ggtggcaggc acggcgaagg
      841 tggtggagcg tatttttctg acggtgtgcg tggtctattt ttcatatgtg atttcggcgt
      901 ttttggccaa gcccgagtgg ctcatctcgt tgttgcacct attgccgccg caattgatgc
      961 actacattcc gacggtgcgg ccgtggattc aggtgccgcc atttaacgcc agctacatgg
     1021 tgattttggc tggtttggtg gggaccacga tagcgccgtg gcagcacttc tatctgcaag
     1081 cggcggtggt ggaaaaacgc gtggggccgc gacaatatgc agcgacccga aatgacgtgc
     1141 tggttggcag tatcagctgc atggtgatcg tgttctttat tatcgtttgc acttccgcaa
     1201 cgctgtttaa caacggtgta cacgaaatcg acaacgcggg aacggcagcg ctggcgctga
     1261 aaccgctggc ggggaagtgg gcggcggctt tgtttgcgct aggcttgttg aacgcttcgc
     1321 tttttgcggc atcgattttg ccgctgtcga cctcgtatgt ggtctgcgaa gcgctgggat
     1381 tcgaatcggg aatcgaccgg aaatttggcg aagccaagat tttttacgcg ctttacacgg
     1441 cgttgattgc gctaggggta gtttttgtgc tgtttttggg gcggttcgcg cagacaattc
     1501 tgctgtggtc gcaagtgctg aacggatttc tgctgcccgt ggtgatgatt tttgttctga
     1561 tcctggtgaa ccggcgcgac ctgatgggcg agaaaactaa tcccaagggc ttcaactggg
     1621 tcgcgtggat ctgcacggtg gccatgatcg ttctgaccct gattgtggcg tactcctcga
     1681 ttcgagaact cttcgggcac gcttcgacct gacgcgccgc gggccgtctg ccggcaacgg
     1741 cggtcggggc cgattgtcct ccgcgaaaca taccgcataa ccccgttgcg gcagctgcaa
     1801 aaggcgtgtt aactttgtgg cataaaggag cgagcgactt ttacaactgt gactgatccg
     1861 aaatccacca aaactctgca cgtcgcagct tcgcacccgc tcggtacggt taccgcagaa
     1921 gaagcattcc tgctagaaaa gctgaatctg agccggttgc cgcggcatgt tgcggtgatc
     1981 atggacggca acggccgctg ggcccaacgc cgccatttgc cgcgcattgc gggacatcgc
     2041 aagggcacag aaaccgcgcg gactacgatt gaaacctgcg cgcgcctgaa gattgaagcg
     2101 ctaacgctct atgcattttc cgtggagaat tggcggcgcc ccaagacgga gattgatttt
     2161 ctgatgcagc ttcttcgcga gtacattcgc caggagatgc cgttgattca gcgcaacgat
     2221 atccggatgc ggtttctggg acgcgccgat gagttgccgg aagaagtgca acgagacacg
     2281 cgagaagcga cggaggcaac cgcggggaat tcaggcatgg tgttgtgcat tgcgctgaac
     2341 tacggcgggc gggcggagat tctggatgcc gccaacgcgc tgcttgcgga acgccgggcc
     2401 gccgccagca ctgcgaagct gacggatgcg gacatcgagc ggcatctata caccagcgga
     2461 ttgcctgatc ccgacctgat gattcgcacc agcggggaga tgcgcgtgag caattttcta
     2521 ttgtggcaga ttgcctacgc ggaaattttc gtgacggaaa cactgtggcc ggattttaat
     2581 cgcgcacggc tgctggaatc gtttgtggaa tttcagaaac gcgaccgccg ctatggcggt
     2641 attggtgacg gcgacgaaag cgaacgtgtc gcttcggaac tcggcgcaaa ataacggctg
     2701 ctgcaccttg taagcccatc ccccgaaata tttctcctgc cggcaaagtc tcccgttatc
     2761 atacgaagtc ttttcatccg ttgagacagg ggaatagctc tcgatgactt ggaaacgtgt
     2821 ggcgaccgcg gtcgtcttga tcccggctgt aatcgggctg gtgctgggag cgccgacggc
     2881 atgggtagcg gtcgcgttgg ccggcgtgat gctgctgtca ctgtttgaat ttttcgcgct
     2941 gggcgatgcg atcgggcatc gcgcgtacaa gttttggacg gcggtctgcg cgtgcctgtt
     3001 ggttttctcg caatatctgg cggcgatgga ttacgcccac tcattgggag acggcctttc
     3061 gctgcaccga attattggac gctttgcggt tcggggcccc acggttgccg atgtgttgtt
     3121 tattttcctg atgggcgttg cggtgctgac gctggcaacg cggcggccgt tggtcgaggc
     3181 gttgccagcg gcgggaatca gttcgagcgc gctgctgctg gtggcgttgc cattgaccta
     3241 ttcgatccgg ctgcacggag tttcgctggc agggaagtgg ttgttgcttt tcgcgctggt
     3301 gattacgtgg attggcgata cggcggcgta ttttgttggg cgggcaatcg ggcggcatcc
     3361 gtttgcgccg cacttgagtc ccaagaaaac gtgggagggc gcagtggcca gctttgcggg
     3421 atcgttgctg gtggcgtggg ccatggcgcg ttggggcaga gtgaatgttg cgacagtgca
     3481 cgttctgggg atggcggcag cgggaaatgt ggccgggcag atgggcgact tgctggagtc
     3541 ggcttacaaa cgcagcgctg gagtgaaaga ctcgggcgcg ctgcttccgg gacatggcgg
     3601 gatactcgat cgcattgatg cgctgatcct gacgtttccg gttgtctggt attattggtt
     3661 gtggatttat tcgccgcggg tgtgagccgc attgaagcag ccagggtcgc tccaggccac
     3721 acaaaatacg ggctttatta cgtgaaggga cttttcgttc ggtgaagcag ctggccattt
     3781 tgggttcgac cggttcgatc ggacggcaga cgctggccgt ggtggaggcg ctcggggagc
     3841 ggtttggcgt ggtggcgctg gcggccggca acaatctgga cgaattagcc ggacagatcg
     3901 agcgctatca gccggaactg gtttccgtcg cggatgcggc gaaagctgat gcgttggcta
     3961 ggcgtttgcg tgaaaaggga attgcgccgt tgcc
//
LOCUS       DASTPM010000664         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_888969, whole genome shotgun sequence.
ACCESSION   DASTPM010000664 DASTPM010000000
VERSION     DASTPM010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888969"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFR42_11710"
     CDS             complement(<1..314)
                     /locus_tag="VFR42_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369873.1"
                     /translation="MAITLAIVGTGFMCALAAAQQAGQTPPSQQAQSAAQAQTQQAAD
                     TATDPQRPALHQRYPRYRIRSSDVIDLTFRFTPEFNQEVTVQPDGYIQARGLPNDVYV
                     QG"
     gene            complement(376..2154)
                     /locus_tag="VFR42_11715"
     CDS             complement(376..2154)
                     /locus_tag="VFR42_11715"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0369874.1"
                     /translation="MDRASVESFELEQPAAFRDSAMFVEFYGLREQPFGVTPDPKFLY
                     LGEGHREALAALFYGLESGRGFSALAAEPGMGKTSLLMRLLESMQETARTAFLFQTEG
                     SSREFLRALLNDLGIVPRGQDLAATHEALNETLLAELNAGRRVIVVIDEAQNLSEKTL
                     ESVRLLSNFETPAAKLMHIILAGQPKLSEKLAKPSLTQLRQRVSNVIQLKPFRSEETV
                     DYIKHRLKMAGLKGQAPFSPDALLLIARSSKGIPRNINNICFQALSLGFATQTQVIGT
                     QIIREVLADLEIETADSSSRRAAESAVPSAPKVAAHWPYIPPQTDRYETNYFSPAPRR
                     SWGMKIFGVLAGAGLTFAVLVLLSANKAKAGFNTEQLRDVVLGTSGGDADQPPALPSR
                     LKPPAGPAIEKSQVATPDAEGLGGQNESAEDAASAPTKGEARAAGRNEEMGDAHVVLL
                     SRAQSTFEIARNYLGRSNWRAVDQIRSLNPQIRGGYQVLPAGTRVVLPGPDPAKRKAG
                     GKLQTSSADANANAEKGKVTFTGNSSLVRVYRQETLFQFALDQYGKSGWEIVSRIRSL
                     NPQLRDPYQVLQQGQWIRVPELAQER"
     gene            complement(2121..>2621)
                     /locus_tag="VFR42_11720"
     CDS             complement(2121..>2621)
                     /locus_tag="VFR42_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HEU0369875.1"
                     /translation="ARLLGGKGIEVFHPTYAAVRQWKDRKKMLQLPLFPGYVFFAGGL
                     DRRVEILSTPGVFSIVSFGGAAAEISGEEIDGIRRAAQGSMAIEPHPFLAEGDRVRVR
                     HGALTGVNGILLRHKDAYRLVLSIELLGRSAAVEIDAAMVERISTLRHRSGYEPWTEQ
                     ALNLSN"
BASE COUNT          521 a          908 c          640 g          552 t
ORIGIN      
        1 atcccctgca cgtacacatc attcggcagg ccgcgcgcct gaatgtagcc atccggctgc
       61 accgtcactt cctggttgaa ttccggcgtg aatcgaaaag tcaggtcaat cacgtcgctt
      121 gagcgaatcc gataacgcgg atagcgctga tgcaacgccg gcctctgcgg atccgtcgcc
      181 gtatccgccg cctgctgagt ttgcgcctgc gctgcacttt gcgcttgctg tgacggcgga
      241 gtttgtcccg cttgttgagc agcagccaga gcgcacataa aacccgtgcc cacaattgcc
      301 agtgtaatcg ccaaaaacct cttacctaga cttggttgga ttttcatcgc cgcaccctcg
      361 ccactgcccg ggccgttacc gctcctgcgc cagctccggc actcggatcc actgcccctg
      421 ttgcaaaacc tgatacggat cacgcagctg cggattcagc gaacggatcc ggcttacaat
      481 ctcccatccc gacttcccgt actgatcgag cgcaaactga aacagcgttt cctgccgata
      541 aacgcgaacc aggctcgaat tgccggtaaa cgttactttt cccttttccg cgttcgcatt
      601 cgcgtccgcg ctgctcgttt gcagttttcc gcccgcctta cgcttggccg gatcgggccc
      661 cggaagcacc acacgcgttc ccgccggcaa aacctgatac cctccgcgaa tctgcgggtt
      721 cagcgaacgg atctgatcga ccgctctcca attcgaacgc cccaaataat ttcgcgcgat
      781 ctcaaacgtg ctctgcgccc ggctgagcag cacgacgtgc gcatctccca tctcctcatt
      841 tctacccgcc gctctcgcct cgccttttgt cggcgcactg gcggcatcct ctgcactttc
      901 gttctgccca cccaggcctt ccgcatccgg cgtcgcgacc tgacttttct ctatagccgg
      961 cccagctggc ggcttcaatc gggacggaag cgccggcggc tgatccgcgt ctccacccga
     1021 cgtgccaagc accacgtccc gcagctgctc agtgttgaag ccggccttgg ccttgttcgc
     1081 gctcaacagc accagcacgg caaaggtcag ccccgcgccc gccagcacgc cgaatatttt
     1141 catcccccac gatcgcctcg gcgccggcga aaaatagttc gtttcatatc gatcggtttg
     1201 aggcggaatg tacggccagt gcgccgccac cttgggtgcc gatggcaccg ccgattcggc
     1261 cgcacgtcgc gaagaactgt ccgccgtttc aatttcaaga tcggccaaga cctcgcggat
     1321 gatttgcgtg ccaatcacct gcgtttgtgt cgcaaatccc agcgataacg cttgaaaaca
     1381 aatgttgttg atgttgcgcg gtatgccctt gctcgaacgc gcgatcagca gcagcgcatc
     1441 gggcgagaat ggcgcctgcc ccttcagccc ggccatcttg agccggtgtt tgatgtagtc
     1501 cacagtttct tcagaacgaa acggcttcaa ctgaatcacg ttcgaaacgc gctgccgcag
     1561 ttgcgtcaaa ctcggcttcg ccagtttttc agaaagcttc ggctgcccgg ccagaataat
     1621 gtgcatcaat tttgccgccg gcgtctcgaa attcgacagt agccgcaccg actcaagcgt
     1681 tttttcactc aggttctgcg cctcgtcgat caccacgatc actcggcgtc cggcattcaa
     1741 ttccgccagt agcgtttcat tcagcgcctc gtgcgtcgcg gccaggtctt gtccacgcgg
     1801 cacgataccc aaatcgttca gcaacgcacg caaaaactcc cgcgaacttc cttccgtctg
     1861 aaacaaaaac gccgtacgcg ccgtctcctg catgctctcc agcagccgca tcagcagcga
     1921 agttttcccc atgcccggct ccgcggccag cgcagaaaac ccgcgtcccg actcaagtcc
     1981 ataaaaaagc gcagccagcg cttcccggtg cccttctccc aggtacagga atttcggatc
     2041 cggtgtcacg ccaaacggct gttcgcgcag cccgtagaac tccacgaaca tggcgctgtc
     2101 gcgaaacgcc gccggttgct ctaattcgaa agattcaacg cttgctctgt ccatggctca
     2161 taacccgatc tgtgtcgcaa agtcgaaatc cgttcgacca tcgctgcatc gatttctacc
     2221 gctgccgatc tgcccagcaa ttcaatcgac aataccaaac ggtacgcgtc cttgtgccgc
     2281 aacaaaatac cgtttacacc tgtcagcgcg ccgtgcctca ctcgtacccg gtctccctcc
     2341 gccaaaaatg ggtgcggctc aatggccatg ctgccctgcg ccgcccgccg aatcccgtca
     2401 atttcttccc cgctgatttc cgctgccgcg ccgccaaacg acacaataga aaacacgcct
     2461 ggggtcgaca aaatctcgac gcgtcgatcc agcccgcccg caaaaaacac gtagcccgga
     2521 aagagcggca gttgcagcat ctttttccgg tctttccatt ggcgcaccgc cgcgtaggtg
     2581 ggatgaaaca cttcgattcc cttaccgcca agcaaacgcg c
//
LOCUS       DASTPM010000665         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_81493, whole genome shotgun sequence.
ACCESSION   DASTPM010000665 DASTPM010000000
VERSION     DASTPM010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81493"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFR42_11725"
     CDS             <1..533
                     /locus_tag="VFR42_11725"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU0369876.1"
                     /translation="STVLSRMCTLIENVTGSVSVVAAAGRYPDFRAMTVPDRWPGEGP
                     LGGIITALRATAQGNGGEWNLIVSCDMPFVTREWLSHVIERTSASDTEVIVPRSEHGL
                     EPLCACWMTAAVDKLHGAFDEGVRKVTEAMKRLRMEVLDETHWKRFDSAGRLFWNMNT
                     PQDYEEAVRIWRTEHE"
     gene            530..1360
                     /locus_tag="VFR42_11730"
     CDS             530..1360
                     /locus_tag="VFR42_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0369877.1"
                     /translation="MSPRDERPASAFSGMNPEGQEQLSENYFEFRDVCKAFDERPVLV
                     DVSFTVKRAETCVIMGRSGVGKSVSLKHIMGFLRADSGQVIVDGEDVTDFSEPKFYAI
                     RKKVTMVFQSGALFDSLTVRDNIAFPLSDQPGLTQADMDAYTDKLAKELEVEEVLDKV
                     PSELSTGMKRAVAIARALAQNPQAILYDEPTTMVDPIMAAHMGDLIQHLKDKFHRTSI
                     VVTHDTHLARRIADTIVFLQEGTASVFASWKEFESSPEPFIQNFIAQDNLIPAMDVTL
                     "
     gene            1357..2823
                     /locus_tag="VFR42_11735"
     CDS             1357..2823
                     /locus_tag="VFR42_11735"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0369878.1"
                     /translation="MSVSSPISSDYLKADPKSQLRKAMGFWDVLFFNIATVLGPRWIA
                     AAGHNGTSSISLWLLAAVFFFIPGAFVINELSSRFPKEGGLYVWAKEAFGDFHGFVAG
                     WTYWIYTVFYFPGLLLASASMSAYLFGGKGAELAQDRTFLIVVSLALLLVAVILNIIG
                     LNIGKWLQNAGGISTYLPLVMLAVIALLVYWRQGSATHFTWANLMPTWNMDTVNFWSQ
                     IAFAFTGLELVSSMSEEVRDPRRTLPRGVFGAGALIAFMYVAGTFAILTLVPAAELDP
                     KSGVFHAITLGSIALKVGFLGVLAAVLVTIGNAGGVGSTVAGIARVPFVVGIDRYLPK
                     VFGQIHPKWKTPHVSMLVQAVASGMILLLSQIQTKTVQDAYQLLILAAIILYFIPFLY
                     MFAAVIKLAGRPDRRANERVVLVPGGKLGVWLAGSCGFLVVLGSIFLSLIPPGEEANK
                     VVFEIKLVGGTLAAILLGLVLYWRGAREKAAEMPRPEA"
BASE COUNT          602 a          729 c          944 g          685 t
ORIGIN      
        1 ggagcactgt tttgtcgcgg atgtgtacgc tgattgagaa cgtcacggga agcgtgagtg
       61 tcgtcgcggc agcgggtcgt taccctgact tccgcgctat gactgtgccg gatcgctggc
      121 ccggtgaagg tccgctcgga ggaatcatca cggcattgcg tgcgactgcg cagggaaatg
      181 gtggcgaatg gaatctgatt gtgagttgcg acatgccgtt cgtcacgcgc gaatggttga
      241 gccacgtgat cgaacggacg agtgccagtg acactgaggt gattgtgccg cggtcggagc
      301 atgggctgga gccgctgtgt gcttgctgga tgacggctgc ggtggacaaa ctgcacggcg
      361 cgtttgacga aggcgtgcgc aaggtgaccg aggcgatgaa acgtctgcgc atggaagtgc
      421 ttgacgagac gcactggaaa cgatttgata gcgcgggacg gctattctgg aatatgaaca
      481 cgccgcagga ttacgaagag gcggtgagga tctggagaac ggagcacgag tgagtccgcg
      541 cgacgagaga ccggcatccg cattttcagg catgaatcct gaggggcaag agcagctttc
      601 cgaaaattat ttcgagtttc gcgatgtgtg caaggcgttt gatgaacgtc cggtactggt
      661 cgacgtgtcc ttcaccgtga agcgcgccga aacgtgcgtc atcatgggac gcagcggcgt
      721 gggcaagtcg gtgtcactga aacatatcat gggatttctg cgcgcggatt ccggccaggt
      781 gattgtggac ggcgaagacg tcaccgattt cagcgagccg aagttctacg ccatccgaaa
      841 aaaagtgacg atggtgtttc agtcgggcgc gctgtttgat tcgctgacgg tgcgcgacaa
      901 cattgcgttt cccctatcag accaacctgg attgacgcaa gccgatatgg atgcgtacac
      961 cgacaaactg gcgaaagaac tggaagtgga agaggtgttg gacaaagtgc cgagcgaact
     1021 ttcgacagga atgaagcgcg ctgtggcgat tgcgcgggcg ctggcgcaga atccgcaggc
     1081 gattttatat gacgagccga cgaccatggt ggatcccatc atggccgcgc acatgggtga
     1141 cctgattcag cacctcaagg acaagtttca ccgcacttct atcgtggtga cacacgacac
     1201 gcacctagca cggcgcattg ccgacacaat cgttttcttg caggagggca cagccagcgt
     1261 gttcgcctcg tggaaagagt ttgaaagttc gcccgagccc ttcattcaga atttcatagc
     1321 acaagacaat ctgattcccg ccatggacgt gactctgtga gcgtctccag cccgatctca
     1381 tctgattatc tgaaggccga cccaaaatca cagcttcgca aagcaatggg attttgggac
     1441 gtactgtttt tcaatatcgc cacagttctc ggtccccggt ggattgccgc ggctgggcac
     1501 aacggaacgt cttccatcag cttgtggcta ctcgcagcag tcttcttttt tattcccggc
     1561 gcattcgtga tcaatgagtt gtcgtcccgc ttcccgaaag aaggtggtct gtacgtctgg
     1621 gccaaggaag cgtttggaga ttttcacggg ttcgtggccg ggtggacgta ctggatttac
     1681 accgtgttct atttccccgg gctgctgctc gccagtgcat caatgagcgc gtatcttttt
     1741 ggcggcaagg gtgcggagct cgcgcaagac cgaacatttc tgatcgtggt ttcgctcgcc
     1801 ttgctcctgg tcgcagtgat cctgaacatc atcgggctga acatcgggaa gtggctgcag
     1861 aatgccggcg gtatctcgac atatctgccg ctggtaatgc tggcggtgat cgcgttgctg
     1921 gtttattgga gacagggatc ggctacgcac ttcacatggg ccaacctgat gccgacctgg
     1981 aatatggaca ccgtgaactt ctggtcgcaa attgcctttg cattcaccgg cctggaattg
     2041 gtgtcttcca tgagcgaaga ggtgcgcgac ccgaggcgta cgttgccgcg aggtgttttt
     2101 ggagcgggcg cgctgatcgc tttcatgtat gtggcaggga ctttcgcgat tctgactttg
     2161 gtgcctgcgg cagaattgga tccgaagagt ggggtgtttc acgccattac gcttggttcg
     2221 atcgctttga aagtgggatt cctgggtgtt ctagcggcgg ttttggtgac gatcgggaac
     2281 gccggcggcg tgggcagcac ggttgcaggg attgcgcggg tccctttcgt cgtaggcatt
     2341 gatcgttatt tgccgaaagt gttcgggcag attcatccga aatggaagac cccgcacgtt
     2401 tcgatgctgg tgcaggccgt ggcatcaggg atgattttgc tgctcagcca gattcagacc
     2461 aagacagtgc aggacgctta ccagttgttg attctggcgg cgatcattct ctatttcatt
     2521 ccgtttcttt acatgtttgc ggcggtgatc aagttggcgg ggcggccgga ccgccgggca
     2581 aatgaacgcg tagtgttggt gcccggaggg aagctgggcg tgtggctggc gggtagctgt
     2641 gggttcctgg tcgtgctggg cagcatattt ttgtcgttga ttccgcccgg ggaagaagca
     2701 aacaaggtcg ttttcgagat caagctcgtg ggcggtacgc tggcggccat tctattgggc
     2761 ttggtgctgt attggcgagg cgcgcgcgag aaggcagccg agatgccacg gccagaagcc
     2821 taaggcgagt agtcctaacc aacgggcggg tgtacccgga tcgtcgcagc aattattgaa
     2881 aatcggcaac ttaccaaaag tcgacttgtt aacttcgacc agccagcgct caaacaaacg
     2941 agattgtgtc gcgcccagcc
//
LOCUS       DASTPM010000666         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1177849, whole genome shotgun sequence.
ACCESSION   DASTPM010000666 DASTPM010000000
VERSION     DASTPM010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177849"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..898
                     /locus_tag="VFR42_11740"
     CDS             455..898
                     /locus_tag="VFR42_11740"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0369879.1"
                     /translation="MSTDAMRPLGATGDRATARLGASLHVKGEITGSEDLLVDGSVEG
                     LIQLDERKLTVGATAKLTADIIAREVVVYGSVKGNLRAKDRIEIKKDGSVNGDLTTAR
                     IMIEDGAYFKGSIEIDKSAEKDSGGSAFAKSSSAPAAAAAGPKSI"
     gene            2201..>2517
                     /locus_tag="VFR42_11745"
     CDS             2201..>2517
                     /locus_tag="VFR42_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0369880.1"
                     /translation="MDLRKLGNSDLQLTPVGFGAWAIGGSGWEFAWGQQEDADSIAAI
                     VRALELGVNWIDTAAVYGTGHSEEIVAQALKQWKGKRPFVFTKCVLRWDDNRKVYQDH
                     SAAS"
BASE COUNT          551 a          696 c          748 g          522 t
ORIGIN      
        1 ggcaaacaca aaaaaccgga tcatgtgcac cgccaatatt gcggcaagaa ggtacccgca
       61 ctgagctgca atgttttggc cacagttagt gcattcgaac gaggcaagtg gcggtttcga
      121 atttgtgttc tgcgctttca aaaatgcaat gaccgttcac gacagcggat agccccttag
      181 tgcagtaacg gaactgcacc aaagggtagt tactttcacg cacagcaagc tgatacgttg
      241 tttgcagcgc ccaacacagg ggaatagagt ttcaaattgc cccgacggaa tctgatgggg
      301 tggccttgtc cctggaggat caatgtgggg gaatgcgaaa aagccggaaa cgccgcaagc
      361 tgcgcaaccg gaaccgaaga gttttgcagc gaatcaaccg aatctgaatc aagcgccgaa
      421 gccagctccg gcgacttggg agggaactaa cgcgatgagt acagatgcaa tgcgtccatt
      481 gggtgcgacc ggcgaccgcg caacggccag actgggagcg agcctgcacg tgaaaggcga
      541 aatcaccggg agcgaagacc tgttggtcga cggctcggtg gaagggttga tccagctgga
      601 tgagcgcaag ctcacggtgg gagcaactgc caagctgact gccgacatca ttgcccgcga
      661 ggtggtggtg tatggcagcg tgaagggcaa ccttcgcgcc aaagaccgga tcgaaatcaa
      721 gaaggatggt tcggtgaacg gcgacctgac cacggcgcgc atcatgatcg aagacggcgc
      781 ctacttcaag ggctccatcg aaatcgacaa gagcgccgag aaggattcgg gcggcagcgc
      841 gttcgcgaaa tcgtccagcg ctccagccgc ggcagcagct ggccccaaga gcatttaacc
      901 caaggtttgt cgcagggagc agggcggcgc gtctgaacta ctgcgccgcc ttgcctgttt
      961 gtgagctcga tatcctccgt ttgcttctag gttgttcagt ttccaggtta ttaatttatt
     1021 ttaagattca ccggcattga acttagtgtg tctgctttga gtccgctgca ggcgtctggc
     1081 atccacattc acggtggcag gtggaagcaa cgcgacattg ctgctcggcg catcgcgact
     1141 cgctgggtca gcgttccgac ccaaactgtt tacgtaacgc tgaccggcca aactttggcg
     1201 ccaacccgac attccgcttt ggaacacaga acgcgacggc cgtcagtagc gccaactcca
     1261 ctcaaataca ggcgactgcg ccggccagcg ggtcactggc gctgtgagtg tcactgcatt
     1321 ctttgcgtac ggtccactcg ccttggcgcc cgccggcttc acttacgggc agcagatact
     1381 ggagtgctcg cgaatgccgg aagcagcagc ggaggcgatc gcatttcgat ttacggctat
     1441 ggcctcggcg aagatcccgc gagattgacg gtaagcctcg cgagttggcg ccgtggtggg
     1501 aattcacgac cagaatttgg ctttgtgctc ggtcatcaaa gactcggaac tggagggcat
     1561 tccgcagcgt accgagaggc ctggaatcgc ctttcatccg agtggcacag tggcgtacct
     1621 tcctttctca caggtccggc tctggcaaac gctccattca ccggtttgct aggcggcgtg
     1681 gacatcctcg acgcaaatac cggccgcctg cgcctgcgga tcattcttcg ggagccgctg
     1741 ccatacgggc cgcggatagc gatggcggcg cggaaaattt ctatttgtcg atggaaatgg
     1801 tcaacggttc ttggcctgga ccgcatccgg attgactgtc gtcgagcttg cccgcctctc
     1861 ggttttggct cgctcgcccc gagcagcgga tccggcggct cgacgatcac gatccgcggc
     1921 tttcagagcg gggccgcggt gaccataggg gaaagccggc ggcaacttcg ttactagaca
     1981 tgaatacgct caagttcacc ctgccggctt ccttaaactt cggctggcaa cgaaccacga
     2041 taggcaatcc cagcggggaa actatttcca ccgctgccgc ctttaacgtg aactagaacg
     2101 tcttgctcca ccggtgttgg cctctctaac ttttcgctgg tgaatttcat ccaaaccggc
     2161 gtagaacatt aacgcaggaa actctcgggc agaggcatac atggatttgc ggaaacttgg
     2221 taactctgat ttgcaattga ctccggttgg gtttggcgcc tgggccatcg gcggctcggg
     2281 ttgggaattc gcttggggcc agcaggaaga tgcagactcc atcgcagcta ttgtgcgcgc
     2341 actggagctt ggcgtgaact ggatcgacac cgccgctgtt tacggcacgg gccattcgga
     2401 agaaatcgtc gcgcaagccc tgaagcagtg gaaagggaag cgcccctttg tttttacaaa
     2461 atgcgttctt cgctgggacg acaatcgcaa ggtctatcaa gatcattccg cagcttc
//
LOCUS       DASTPM010000667         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_24208, whole genome shotgun sequence.
ACCESSION   DASTPM010000667 DASTPM010000000
VERSION     DASTPM010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24208"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1555
                     /locus_tag="VFR42_11750"
     CDS             254..1555
                     /locus_tag="VFR42_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0369881.1"
                     /translation="MIDVRTGVLHAGQIIVIQQKEIVAVGTSDKIKIPPKVKQVDCTG
                     LFAIPGLWDMHVHLVFGDWFPGGKEISLPMFVANGVTGVRDMGSDLEPVKNWRSQIAA
                     GKLIGPRIYTPGPMLDGPKPRFPSSVAIATPEDARRAVDDLKSRGADFIKLQSLIPRD
                     AVFAIADEAKKQGIPFEGHVPDAVRASEMSDAGMKSFEHLIGIFEGSSPSEDDFLQGG
                     KTEGKFLATFEPARAEKLSAQLANHRTWQCPTLVWERGGNLVDVTDFSADARANYVPP
                     SWKAVTWKRFRQEITSGYGGDNLQVRNKFITKELELVGMMRAKGVDFLAGTDTPAGVY
                     VFPGFSLHEELQRFVAAGFTPLQALQTATVNPARFFGEEAHFGAVEAGKAADLVLLRA
                     NPLDNISNTQQIAGVVLNGRYFSRGELDALLDRVKAAASNQ"
     gene            1577..2032
                     /gene="tsaA"
                     /locus_tag="VFR42_11755"
     CDS             1577..2032
                     /gene="tsaA"
                     /locus_tag="VFR42_11755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEU0369882.1"
                     /translation="MPLPRASRKNSRTKNRQPWSLRPIGVIRSTLRERSEAPKQGSEG
                     APDAWLDVQNFALSALDGIVPGDDLLILTWLHQADRTVLKVHPRSDPSRRLTGVFATR
                     SPDRPNPIGLHRITVRKIEKHRLLIGPMEAIDGTPVVDIKPALSCAPDF"
BASE COUNT          499 a          731 c          627 g          429 t
ORIGIN      
        1 tccttgaagc tccggcaacc ttacgaggtg ccggagcttt tcatttcgga cgccgctaac
       61 ggagaaagga gccgaaagaa cagatagaat gcgctgcatg agaccttcgg cacaaacagg
      121 acggcgcaag accctgattt cttcgttttt tctaatcgtt gcgcttctta gctttgcgca
      181 acctcttttt ccccagtccc cgccgccgtc tgcggattca cttcccgttt cagttgtgct
      241 gacgcatgtc agcgtgattg acgtgcgaac cggtgtcctg cacgctggcc aaatcatcgt
      301 catacagcaa aaagagatcg tcgcagtagg aacgtccgac aaaataaaaa ttcctccgaa
      361 agtaaagcaa gtggattgca ccggactgtt cgcaattccg ggcttgtggg acatgcacgt
      421 gcacctggtc ttcggcgact ggttcccggg cggcaaagag atttccttgc cgatgtttgt
      481 cgccaacggc gtaactggcg tgcgcgacat gggcagcgac ctggagcctg taaaaaactg
      541 gcgcagccag atcgcagcgg gcaaactgat cggaccgcgc atctatacgc cgggccccat
      601 gctggatggg ccaaagccgc gctttcccag ttccgtcgca attgcgacgc cggaagacgc
      661 acgccgcgcc gtcgatgacc tcaaaagccg cggcgcggat ttcatcaagc tgcaatcgct
      721 gattccgcgc gatgcagtgt tcgctatcgc cgacgaagcg aagaaacagg gcattccgtt
      781 cgaaggccac gtgccagacg cagtgcgggc cagcgaaatg tccgatgccg gaatgaagag
      841 cttcgaacac ctgattggaa ttttcgaggg cagctcacct tccgaagacg attttttgca
      901 gggcggcaag accgaaggca aattcctggc aaccttcgaa cccgcccgcg cggagaaact
      961 ttcggcgcag ctcgcaaacc atcgcacctg gcaatgtccg acgctggtgt gggagcgcgg
     1021 cggaaatctg gtggacgtca cagacttttc ggcggacgcg cgcgccaact acgtgccccc
     1081 atcctggaaa gcggtaacct ggaagcgatt ccgccaggaa attaccagtg gctatggcgg
     1141 tgacaatttg caagtccgca acaaattcat cactaaggaa ctggaactgg tgggcatgat
     1201 gcgcgcgaag ggcgtggatt ttttagcggg gacagacaca cccgcgggag tgtatgtctt
     1261 tcccggcttc agtctgcacg aagaactgca aagatttgtg gccgcgggtt tcacgcctct
     1321 gcaagcgctg caaacggcaa ccgtcaatcc cgctcgcttt ttcggcgaag aagcccactt
     1381 cggtgccgta gaagcaggaa aagcggcgga cctggtgctg ctgcgagcta atccgctgga
     1441 caacatcagc aacacgcagc aaatcgcagg cgttgtcctg aacgggcgct atttctcgcg
     1501 cggagagcta gacgcgttac tggacagagt caaagcagcc gcatcgaatc aatagctctc
     1561 agaaggtgaa accacgatgc cactccctag agcaagccgc aaaaactcgc gcaccaaaaa
     1621 tcgccagccc tggagcctcc gcccgattgg cgtgattcgc tcgaccctcc gcgaacgcag
     1681 cgaagccccg aaacaaggct ccgaaggcgc acccgacgcg tggctcgacg tacaaaactt
     1741 tgctctctcc gctctcgacg gaattgtgcc cggcgacgac ctcctcatct tgacctggct
     1801 gcaccaagcc gaccgcactg ttctgaaagt ccatccgcga tccgacccca gccgccgcct
     1861 aaccggcgtc tttgccacgc gctccccgga tcgccccaat ccaatcggcc ttcaccgcat
     1921 caccgtgcgc aaaatcgaga agcatcgtct gctcatcggc cctatggaag ccatcgacgg
     1981 cactcccgtg gtggacatca agcccgccct ctcctgcgct ccggatttct gatgcccgct
     2041 ccgcacccgc cccgggcctt gcgatactcg aaggttaacg tcctgtctat tgccatgccg
     2101 atacgaactc accagccatg tttcccagcg aactttggca ccgcaccaat cgggattgtc
     2161 aggcatcccc ccggcacagc ccgtcggcac agcccgtcgg acccgaaggt gagccgcggg
     2221 taatccgtgg ccgcccttcc cacgccttcg ccgcaggaca cccgggcgcg ctcctggcgc
     2281 ttccgc
//
LOCUS       DASTPM010000668         6948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_831257, whole genome shotgun sequence.
ACCESSION   DASTPM010000668 DASTPM010000000
VERSION     DASTPM010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831257"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VFR42_11760"
     CDS             complement(<1..1636)
                     /locus_tag="VFR42_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEU0369883.1"
                     /translation="MKPAIDAWLPKSVILLRTYDRQKFLADLTAGITVGLVALPLAMA
                     FAIASGVPPQNGLYCAIVTGFVISALGGCKTQIGGPTGAFVVVVAGIIAKHGIEGLFL
                     CTMMAGVLLVLLGVTGMGTAVKYFPRPVVVGFTNGIAILIASTQIRDFFGLRIEQLPA
                     DFFHRMAALARALPTLNWQAASVALISLTVMILCLKYAKRIPGAILVCFGATAVISLF
                     HLGVETIGTRFGGIPTGLPQIVVPQFHYDTARELFSAALTVAMLGAIESLMSAVVSDK
                     MSSDKHNPNVELIGQGIANILSPLVGGLPATGAIARTATNVRAGAKTPVAGMIHALTL
                     LAIILFAAPLAKNVPLSALAAILMMVAYNMGDWEEIPEILKHSWADIAVWLLTLTLTV
                     VTDLTFAMEVGMLIAALTYIRKVSRTTTVTRVTRDYVADSSVHVLQGKDIPDYAVVYR
                     VHGPFLFGATDKFDEIIDRIDTLPPIVVLRLRNMTAIDATGLGAIRELADRLHATGRH
                     LLLCGAREQPAQLMQQAEFERHVGAENMCHSITDAIS"
     gene            complement(1743..3254)
                     /gene="gcvPB"
                     /locus_tag="VFR42_11765"
     CDS             complement(1743..3254)
                     /gene="gcvPB"
                     /locus_tag="VFR42_11765"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEU0369884.1"
                     /translation="MTDPIKKATRHTNQNEGLVFERSSPGKRAFELPPLDVPATDAAK
                     SLGAHHRSNGVEGFPEVSEIEVIRHFTRLSTWNYAIDLGMYPLGSCTMKYNPRVNEFV
                     ARLDGICTEHPYQPQELSQGCLKILQLLENCLLEITGMDAITLQPAAGAQGELTGLLL
                     IRAYLEKQGNARKKVLIPDSAHGTNPATAVIAGYQVENIKSESHGQIDVDKLRDLVTE
                     EVAALMITNPSTLGIFEERIPEIAEILHARGALLYMDGANMNALTGVTRPGDLGVDVM
                     HLNLHKTFSTPHGGGGPGAGPVAMKKILEPFRPVPILVEKDGGRLALETVRPSSIGKV
                     RAFGGNFGVLVRALAYILAYGPGVRQATEDAVLNANYIRKHLEGVFDLPYSLPSMHEV
                     VFSDAIQKRNGVNTMDIAKRLIDYGFHPYTTAFPLIVPGALMIEPTESESKEECDLFI
                     DAMKNIAEEAAATPELVKSAPHTTRVRRLDETAAARKPILRWKPSAAKTSAAG"
     gene            complement(3284..4630)
                     /gene="gcvPA"
                     /locus_tag="VFR42_11770"
     CDS             complement(3284..4630)
                     /gene="gcvPA"
                     /locus_tag="VFR42_11770"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEU0369885.1"
                     /translation="MRYLPKSPAERQEMLAAIGAKSIDELFACIPQRFRLRDALNLPG
                     PLSEAEVISYFKDRAGENSVGYSSFLGAGVYNHLRSVITDTILLRGEFLTSYTPYQAE
                     ITQGTLQAIFEFQTLMCQLTGQEVANASMYDGSTATTEAVLMAERLTGRGRVLVARSM
                     HPEYREVLRTYAKNSGLHIEEIGYIEDGTVDAAALKAALKDDVAAVVLQSPNFFGVLE
                     TPAGIADSAHAAGALLVVAVAEGVSLGAVKPPAEADIVAMEAQSFGLPPSYGGPFAGV
                     VASRDKFVRQMPGRLAGQTLDVAGQRGFVLTLATREQHIRREKATSNICTNQALCALA
                     ATVHLTLLGKEGLREMAEQNLAKAQFTLSLLEKIPGVRRSFAGPFFNEFTLEFPRSVR
                     MINGDLLKEKIVGPLALGPYYPELSKHGLVCVTETTSRAEIERFAQAVERILAKAA"
     gene            complement(4630..5031)
                     /gene="gcvH"
                     /locus_tag="VFR42_11775"
     CDS             complement(4630..5031)
                     /gene="gcvH"
                     /locus_tag="VFR42_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU0369886.1"
                     /translation="MAYPANYRYTKEHEWVEVKNGVATVGITDYAQSELGDVVFVELP
                     EPGAKLTKGKSFGTVESVKAVSELYAPVSGEVIEANGELQNKPETINSDPHGAAWLIR
                     IKLVSPAELDGLMDGSAYQAYIAAKGQEASA"
     gene            complement(5059..6240)
                     /gene="gcvT"
                     /locus_tag="VFR42_11780"
     CDS             complement(5059..6240)
                     /gene="gcvT"
                     /locus_tag="VFR42_11780"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU0369887.1"
                     /translation="MDSGISPTVTELRNTPLNALHRRLGARMVNFGGWDMPVEYPATG
                     GLMAEHKAVRTSVGVFDVSHMGDIRIRTGKTLGGALAAVQHISMNDASSLAIGQAHYS
                     AMLYPEGTFVDDVIVHRLAEDDFLFVINAGTRQKDISWVRDNVKASDCVVEDLSDAYT
                     QLAIQGPRAADVLQKLTDVDLAPIRNYWFTRGTVCGLPNCMIARTGYTGEDGFEIYVP
                     GDVATSEMVWNAVMDTGKEFGILPCGLGARNTLRLEAKMALYGHEISEKINVWEAGLD
                     RYCKMEKPEFVGRAALAKASANGLKRTLVGLEMIERGIARDEYKVFDAAGEEVGVVTS
                     GSPSPTLSKNIALAYVPPALAALGTTLMVEVRSQRVKAQVVATPFYKRPRKAASQAVT
                     N"
     gene            6344..>6948
                     /locus_tag="VFR42_11785"
     CDS             6344..>6948
                     /locus_tag="VFR42_11785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0369888.1"
                     /translation="MPPSFRAARDDALRQSFAPLLILLGLAIVINYVDRGNLSIAAPI
                     IKNELKLSATQLGLLFSAFFYTYTALQFVVGGVVDRFGANRVLSTGLVLWSLATIAMG
                     FAGGFIALLAWRLLLGVGESVAFPCTSKVIAQHIPAKNRGLANGVVTAGLKFGPAVGA
                     FGAGMLIAKYGWRPVFWGMGIVSLLWLPAWWRWSPREGASRG"
BASE COUNT         1372 a         1758 c         2371 g         1447 t
ORIGIN      
        1 gggagatagc gtcggtgatg ctgtggcaca tgttttcggc gccaacgtgg cgttcgaatt
       61 cggcttgctg catgagttgg gcgggctgtt cgcgggcgcc gcaaagcagg agatggcgac
      121 ctgtggcgtg gagacggtcg gccagttcac ggatggcgcc gagcccggta gcgtcgatgg
      181 cggtcatgtt gcggaggcga aggacaacga tggggggcag ggtgtcgatg cgatcgatga
      241 tttcgtcgaa cttgtcggtg gcaccgaaga gaaaaggtcc gtgaacacgg tagacgacgg
      301 cgtaatcggg aatgtccttg ccctgcaaga cgtgcacgga gctgtcagca acataatcgc
      361 gagtcacacg cgtgacggtg gtggtgcgag aaactttgcg gatgtaggtg agcgcggcga
      421 tgagcatgcc aacttccatg gcgaaagtca ggtcggtgac gacggtgagc gtgagggtga
      481 gcagccagac ggcgatgtca gcccaggagt gcttgaggat ttcggggatt tcctcccagt
      541 cgcccatgtt gtaggcaacc atcatcagga tggcggcgag ggcggagagt ggaacgtttt
      601 tcgccagcgg ggcagcgaac aaaatgatgg cgagaagcgt gagggcgtga atcatgccgg
      661 ccacgggagt ctttgcgccg gcacggacat tggtggcggt gcgggcaatg gccccggtgg
      721 cagggaggcc gccaaccaaa ggagagagga tgttggctat gccctggccg atgagctcga
      781 cattgggatt gtgcttgtcg ctagacattt tgtcggagac gaccgccgac atgagcgact
      841 cgatggcgcc gagcatggct acggtgagtg cagcggagaa aagctcgcgc gccgtgtcgt
      901 agtgaaattg gggcacgaca atctgcggaa ggccggtggg gatgccgcca aagcgcgtgc
      961 cgatggtttc cacaccgaga tggaacaggg aaatcacagc ggtggcgccg aaacaaacca
     1021 ggatggcacc ggggatgcgt ttggcgtact tcaggcagag aatcattacg gtaagtgaga
     1081 tcagggcgac agaggctgcc tgccaattca gagtgggcag agcgcgggcc agagcggcca
     1141 tgcgatggaa gaaatccgca ggaagctgtt caatgcgcag gccgaagaaa tcgcggattt
     1201 gcgtgctggc gatcagaatg gcgatgccgt tggtaaagcc gacgacgacc gggcggggaa
     1261 aatatttgac ggcggtgccc ataccggtga cgccgagaag gacgaggaga acgccggcca
     1321 tcatggtgca gagaaagagg ccttcgatgc cgtgtttcgc gatgatgccg gcgacgacga
     1381 cgacgaaggc accggtaggg ccgccgattt gcgttttgca gccgcccagc gcggaaatta
     1441 cgaatcccgt gacgatcgcg cagtagagac cgttctgcgg cgggacgccg gaggcgatgg
     1501 cgaaagccat agcgaggggg agagcgacca ggccgacagt gatgccggcg gtgaggtcag
     1561 caagaaattt ttgacggtcg taagtgcgaa gaaggattac cgattttggt aaccaggcat
     1621 cgatggcggg tttcatcggg ttcgttcgca gcgttgaatc gcatcatagc aaacctatgg
     1681 acggcggcgc gtttgagtac gggcaatgtg tcgggttgaa aatcacgacc gcgtcggtcg
     1741 ggttaaccgg cggcgctggt tttggcggcc gacggcttcc agcgcaggat gggcttgcgg
     1801 gcggcggcag tttcgtcgag acggcggacg cgcgtggtgt gcggagcgga cttgacgagt
     1861 tcgggagtgg ccgcggcttc ttcggcgata tttttcatgg cgtcgatgaa gaggtcgcac
     1921 tcttccttgg attcagattc ggtgggctcg atcatcagag cgccgggaac gatcagagga
     1981 aacgccgtgg tgtaggggtg gaagccgtag tcgatgaggc gcttggcgat gtccatggtg
     2041 ttgacgccgt tgcgtttctg gatggcgtcg ctgaagacga cttcgtgcat ggagggcagg
     2101 gagtagggaa gatcgaaaac gccttcgaga tgtttgcgga tgtagttggc gttgaggacg
     2161 gcgtcttcgg tggcctgacg gacgccgggg ccataagcga ggatgtaggc gagggcccgg
     2221 actaagacgc cgaaattccc gccgaaggcg cgaaccttgc cgatggatga cgggcgaaca
     2281 gtttcgagag cgagccggcc gccatctttt tctacgagaa tcggaacggg gcggaaaggc
     2341 tcgaggatct ttttcatcgc gacggggccg gcacccgggc cgccaccgcc gtgcggcgtg
     2401 ctgaaagttt tgtgcaggtt gaggtgcatc acgtcaacgc ccagatcacc ggggcgagtg
     2461 acgccagtga gggcgttcat attggcgccg tccatataga gaagagcgcc cctggcgtgg
     2521 aggatttcgg cgatttccgg gatgcgttct tcaaagatac cgagcgtaga gggattggtg
     2581 atcatcagcg cggctacctc ttcggtgacg aggtcgcgga gtttgtccac gtcgatctgg
     2641 ccgtgcgact cggacttgat gttttcgact tggtagccgg cgatgaccgc ggtggcggga
     2701 ttggtgccat gggcggagtc cggaatcagg acttttttgc gggcgttgcc ttgtttttcc
     2761 agataggcgc ggatcaggag aaggccggtg agttcgcctt gcgcgccggc ggcgggctgg
     2821 agcgtgatgg cgtccatgcc ggtgatttcg agaaggcagt tttcgaggag ttggagaatt
     2881 ttgaggcagc cttgcgagag ttcctgtggc tggtagggat gttcggtaca gatgccgtcg
     2941 aggcgggcga caaattcgtt gacgcgcggg ttgtacttca tggtgcagga accgagcggg
     3001 tacatgccga ggtcgatggc gtaattccac gtggacaggc gggtaaagtg gcggatgact
     3061 tcgatttcgc tgacttcggg aaaaccttcg acgccgttcg agcggtggtg cgcaccgagg
     3121 gatttcgccg catccgtggc aggcacgtcg agcggaggca actcgaacgc gcgcttgccg
     3181 ggggaagaac gttcaaagac cagcccttcg ttctgattgg tgtggcgggt cgcttttttg
     3241 attgggtcag tcattcggtt cgtttctctt aaaaattgtc atgctaggcc gctttggcca
     3301 agatacgttc gacggcttgc gcgaagcgtt caatttccgc gcgcgaagtg gtttcggtga
     3361 cgcagaccag gccgtgcttg ctgagttcgg gataatacgg gccgagggcc agcgggccga
     3421 cgattttttc tttcagcaga tcgccgttga tcatgcgaac agagcgggga aattccaaag
     3481 tgaactcgtt aaaaaagggt cctgcgaagg aacggcggac gccggggatc ttttcgagaa
     3541 gcgacagagt gaactgagcc ttggccagat tttgctcggc catttcacgc aggccttcct
     3601 tgccgagcag cgtcagatgg acggtggcgg cgagagcgca aagggcctga ttggtacaga
     3661 tgttggaagt ggctttttcg cgacggatgt gctgttcgcg ggtggccagc gtgagcacga
     3721 agccacgctg gccggcgacg tctaaggttt gccccgcgag acggccgggc atctggcgaa
     3781 caaatttgtc gcgcgaggcg actactcccg cgaagggacc gccgtaactg ggcggcaggc
     3841 cgaagctctg cgcttccatg gcgacgatgt cggcttcggc gggcggcttg accgcgccga
     3901 gcgaaacgcc ttcggcgacg gcgacgacga gtaaagcgcc ggcggcatga gccgagtcgg
     3961 cgatgccggc aggtgtttcg agaacgccaa aaaagttggg tgattggaga acgacggcgg
     4021 cgacatcatc tttgagtgcg gctttcagtg cagcagcgtc caccgtgccg tcttcgatgt
     4081 agccgatttc ttcaatgtga aggccggaat ttttggcgta ggtgcgcagg acttcgcgat
     4141 attcggggtg catggagcgc gccaccagga ctcggccgcg cccagtgagg cgttccgcca
     4201 tgaggacggc ttcggtggtc gcggtcgaac cgtcgtacat ggatgcgttg gcgacttcct
     4261 ggccggtgag ctggcacatt agcgtttgga attcgaagat ggcttgcagt gtgccctggg
     4321 tgatttcggc ctgatacggc gtgtaggaag tcaggaattc gccgcgcaac agaatggtgt
     4381 cggtgatgac cgaacgcagg tggttgtaaa cgccggcgcc gaggaaactg ctgtagccga
     4441 cagaattctc gccggcgcga tctttgaagt aagaaatgac ttcggcttcg cttagagggc
     4501 cgggcaagtt gagcgcgtcg cggaggcgga agcgctgtgg aatgcaggca aaaagctcat
     4561 cgatggattt tgcgccgata gcggcgagca tttcctgccg ctcggcggga ctcttcggaa
     4621 gatagcgcat caggccgagg cttcctggcc tttcgccgcg atgtaggctt ggtaggcgga
     4681 gccgtccatc agaccgtcga gttcggcggg actcaccagt ttaattctta tgagccaggc
     4741 agcgccgtgc gggtcggagt tgatggtttc gggtttgttc tgcaactcgc cattggcttc
     4801 gatgacctcg ccggaaaccg gcgcgtaaag ctcgctcacg gctttgaccg actcaaccgt
     4861 gccaaaagat ttccccttgg tgagttttgc gccaggctca ggaagttcga cgaacaccac
     4921 gtcgcctagc tcgctctgcg cgtaatcggt aatgccgacg gtggcgactc catttttaac
     4981 ctccacccat tcgtgttctt ttgtgtagcg atagtttgcc ggataagcca tgtgaccact
     5041 ccttgccaag aaattgggct aattcgtgac cgcttgcgat gccgccttac ggggccgctt
     5101 ataaaacgga gttgctacta cttgcgcttt cacacgctgt gaacgcactt ccaccataag
     5161 agttgtgcca agggccgcga gcgccggagg aacataagcc agcgcgatgt ttttggaaag
     5221 ggtcggagag ggcgagccgc tggtgacgac gccaacttcc tcaccagccg cgtcgaagac
     5281 tttgtactcg tcgcgagcga tgccgcgttc gatcatctcg agtccgacta gcgttctctt
     5341 tagtccattg gcggacgctt ttgccagggc cgcgcgaccg acaaattccg gcttttccat
     5401 tttgcaataa cggtcgagac ccgcttccca cacgttaatt ttttcgctga tctcgtggcc
     5461 gtagagcgcc atcttggctt cgagacgaag cgtattgcgt gccccaaggc cgcagggaag
     5521 gatgccaaat tccttgcccg tatccatcac cgcattccag accatttcac tggttgcgac
     5581 gtcaccggga acgtaaatct caaagccgtc ttcgccggta tagccggtgc gggcgatcat
     5641 gcagttcggt aaaccgcaga cggtaccgcg ggtgaaccag taatttctaa tcggagcaag
     5701 atcgacgtca gtaagtttct gcagaacatc cgcggcgcgc ggaccttgaa tcgccagttg
     5761 cgtataagcg tcgcttagat cttcgacgac gcaatcggat gcctttacgt tgtcgcgaac
     5821 ccaggaaata tctttttggc gagttccggc gttgatgaca aagagaaaat cgtcttcagc
     5881 gaggcggtgg acgatcacgt cgtccacaaa agttccttcc ggatagagca tagccgaata
     5941 gtgcgcctgg ccgatggcga gactcgaagc atcgttcatg ctgatgtgct gaacggccgc
     6001 cagtgcgcct cccaacgttt ttcccgtgcg aatacgaatg tcgcccatgt ggctcacgtc
     6061 aaagacgccg acagaagtac gcacggcttt gtgctcggcc atcaagcccc cggtcgcggg
     6121 gtattcgacc ggcatatccc atcccccgaa attgaccatg cgagctccca gccggcggtg
     6181 caatgcattc agcggcgtgt tacgaagttc ggtaacggtg ggagagattc cagagtccat
     6241 tctgggtgct caggcagaaa tcgatactag catgcgcgat tcaggcggtc aaaaagcggg
     6301 aaccggttgc caatcgacgc tgacgaacac ataatgcgtc gcaatgcctc cctccttccg
     6361 cgcggcgcgc gacgatgctt tgcgacaatc ctttgcgccg ctgctcattc tgctgggtct
     6421 ggcgattgtg atcaattatg tggatcgcgg aaatctctcg attgccgcgc cgatcatcaa
     6481 gaacgagcta aagctttccg cgacgcagct gggcctcctg ttttccgcgt tcttttatac
     6541 ctacacggcg ttgcagtttg tggtcggcgg agtagtcgac cgttttggtg cgaaccgcgt
     6601 tctgagtacc gggctggtgc tgtggtcgct tgcgaccatc gcgatgggat tcgcgggagg
     6661 cttcattgct ttactggctt ggcgattgct acttggcgtg ggggaatcgg tggcgtttcc
     6721 gtgcacgtcc aaagtgattg cacagcatat accggcgaag aaccgcgggc tcgccaacgg
     6781 cgtggtgacg gcgggactga agttcggccc ggcggtgggc gcgttcggcg cggggatgct
     6841 gatcgcgaaa tatggctggc ggccggtgtt ttggggaatg ggtatcgtga gcctgctgtg
     6901 gcttccggcg tggtggaggt ggagtccgcg ggaaggagca agccgagg
//
LOCUS       DASTPM010000669         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_197165, whole genome shotgun sequence.
ACCESSION   DASTPM010000669 DASTPM010000000
VERSION     DASTPM010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197165"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VFR42_11790"
     CDS             <1..1639
                     /locus_tag="VFR42_11790"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0369889.1"
                     /translation="IQAEGGKRRGALGQRVPLGSGLIGNVARAGNLVTFRSSLAADAA
                     MRPMLADSAAAVGLPIFFAEQLHGVLYVESSDNIDFTEEEVLLLGTLADLIAGALHNA
                     LTFQKAQEQAITDGLTGVKTHRFFMEALSAEWKRSTRAGRAFALVLMDLDRFKFVNDF
                     YGHLEGDLVLQRVGHILEANCRRSDVVARYGGDEFVILMPETSMEQARQLASKLRSWV
                     CADNLLREKNVSASFGIACYPLHGSSPQELIQVADASMYLSKHQGGNAVSTADHFDPS
                     EAKKWKKDVLEAYLGVTLKRLFSTGPEAFEEIYQRLKQFTESLAATEAGNGVTLVQSS
                     PESAGPQALPQAVLDTVTSLAFAIDAKDHYTQGHSQKVSAYAALIAEAMDMRDAEIEE
                     IRLGAVLHDIGKVGIPEQILNKSGPLNPDEWETMKSHVVFGAKILEPLTPLARVREMV
                     HHHHEYFDGSGYPHSLKGEQIPLGARIIAVADAYDTITSDRTYKKGRSAADALAELER
                     CANAQFDAGIVELFVRVMRQQANPIIEVASLTQERNA"
     gene            complement(1666..1875)
                     /locus_tag="VFR42_11795"
     CDS             complement(1666..1875)
                     /locus_tag="VFR42_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369890.1"
                     /translation="MKSGEPIIPSLAKLPVLVYTSLDPKTCYDAADGPGISGHVVEPL
                     NAQELLEGVGWCWIRQAASCFLVSV"
     gene            complement(1967..>2163)
                     /locus_tag="VFR42_11800"
     CDS             complement(1967..>2163)
                     /locus_tag="VFR42_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369891.1"
                     /translation="QTWQEDCKKVDKRVWLAAKESKGVEGQNVGSMFCYGTHFSLERE
                     FCRETVNRGLGEFREDSYRG"
BASE COUNT          447 a          698 c          551 g          467 t
ORIGIN      
        1 gatccaagcc gaaggtggta aacgccgcgg cgcgttaggg caacgcgtcc cgctcggctc
       61 cggactgatc ggcaatgtcg ctcgcgccgg aaatctcgtc accttccgtt ccagccttgc
      121 cgccgacgcc gccatgcggc ccatgctcgc cgattccgcc gcggccgtcg gtttgcccat
      181 ctttttcgcg gaacaacttc acggcgtact ttacgtcgaa tcttccgaca atatcgattt
      241 caccgaggaa gaagtcctgt tgctcggaac gttggccgac ttgattgccg gcgctctcca
      301 caacgctctc acgttccaga aagctcagga acaagccatc actgacggcc tcaccggcgt
      361 caaaacccat cgcttcttta tggaagcgct ctccgccgaa tggaagcgct ccactcgcgc
      421 tggccgtgct ttcgccctgg tcctcatgga cctcgaccgc ttcaaattcg tcaacgactt
      481 ctacggtcac ctcgaaggtg atttggtgct gcaacgtgtg ggtcacatcc tcgaggccaa
      541 ctgccgccgc tctgacgtgg tagcccgtta tggcggcgac gaattcgtaa ttctcatgcc
      601 cgaaacctcc atggagcagg ctcgccagct cgcttccaag ctgcgcagct gggtttgcgc
      661 ggacaatctt cttcgcgaaa agaatgtctc cgccagcttt ggcattgctt gttatccctt
      721 gcatggttcg tccccgcagg aacttatcca ggtcgcggat gcctccatgt acctctcgaa
      781 gcaccagggc ggcaatgccg tttccaccgc ggaccatttc gatcccagcg aagccaagaa
      841 atggaaaaag gacgttctcg aggcctatct aggcgtcacc ctcaaacgcc tcttttccac
      901 cggccccgag gccttcgaag agatttatca gcggctcaaa caatttaccg aatcgctggc
      961 cgccaccgaa gcgggtaatg gggttacgtt agtgcaatcg tcgccggagt ccgccgggcc
     1021 gcaagcattg ccgcaggctg ttctggatac cgtcacgtct ctggcgttcg cgatcgacgc
     1081 caaggaccac tacacccaag gacactcgca aaaggtttcg gcttacgccg cgctgatcgc
     1141 cgaagccatg gacatgcgcg atgcggaaat cgaagagatt cgcctcggag ccgtgctgca
     1201 cgacatcgga aaagttggca taccggaaca gattctgaac aagtccggac ctttgaatcc
     1261 tgacgagtgg gaaaccatga agtcccacgt ggttttcggc gcaaaaattc tggagccgct
     1321 gaccccgctt gcccgcgttc gtgaaatggt acatcaccat cacgaatatt ttgacggctc
     1381 aggctatcct cattccctca agggggaaca gattcctctc ggcgcccgca tcatcgccgt
     1441 tgccgacgcg tacgacacca tcaccagtga ccgtacgtac aagaaaggcc gcagtgcggc
     1501 cgacgccttg gccgaactgg agcgctgcgc caacgcgcag ttcgacgccg gtatcgtgga
     1561 actcttcgtt cgcgtgatgc gccagcaagc caaccccatt attgaagttg cctctctgac
     1621 tcaggaaaga aacgcctaaa ggcgattgtt ctcgggagcg cgacgttaga cggatacgag
     1681 aaagcagctc gctgcctgcc gaatccagca ccaacccaca ccctccagca gttcttgtgc
     1741 gttcaacggc tccaccacat ggcccgaaat tcccggacca tcggccgcgt cgtaacatgt
     1801 tttcgggtcc agcgatgtat agaccagcac cggcaacttc gccagggacg ggatgattgg
     1861 ctcgccgctt ttcaaaatct ccttggtctt ctcccgcgct ctcgcacaat tcgtccccta
     1921 gcccgcaatt gccagggctc tgcccggcgt ccggattaaa cggtcgttat ccacgataag
     1981 aatcttcacg gaattcccct agtcctcgat tcacagtttc gcgacagaat tcgcgttcca
     2041 gtgagaaatg agtcccgtag cagaacatcg aaccaacgtt ttgcccctcg acgcctttgc
     2101 tttctttggc cgctagccac actcttttgt ccactttctt gcagtcctcc tgccaagttt
     2161 gtc
//
LOCUS       DASTPM010000670         3304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_889131, whole genome shotgun sequence.
ACCESSION   DASTPM010000670 DASTPM010000000
VERSION     DASTPM010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889131"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1605
                     /locus_tag="VFR42_11805"
     CDS             <1..1605
                     /locus_tag="VFR42_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0369892.1"
                     /translation="FVLLIAGVNVANLLLARSTGRAREFAIRMALGAGRGRILRQLLT
                     ESLLLAAIGGGLGVLLAALGTKAALRVLPETLPRAAEVGIDARVLVFAAGVSLLAGIC
                     FGLLPALKISKSGAQTALKEGGRGGSGTRHRVQRALVAAEMALALVLLIGAGLMIRTL
                     GALWNVNPGFEPKNVLTFGFALPSAMSGANADSVRAALREVHEKFAAAPAIQAVSFSW
                     GALPLSSDDEWLFWIDGQGKPANDNDMNWALNYVVEPDYLRVMGIPLKRGRFFTSQDD
                     ERGQKVAVIDEALASKFFSGIDPVGKRLHLNNGGDQLVEIVGVVGHVKQWSLDADQKE
                     SLQAQLYTPFMQLPDKAMAQSASGISVLIRSQNTASVFDSVRRASTEMSNQQVVFGAQ
                     TMNDIIANSLAERRFSMTLLGIFAGVALGLASIGIYGVVSYLVGQRTQEIGVRIAMGA
                     QRSQVLGLVLAQGAAMSAIGAAIGLGAALPLTRLMGTLLFAVSTADPLTYLCVVAILL
                     VVSMAACWLPAHRAARMNPVDALRYE"
     gene            1767..2414
                     /locus_tag="VFR42_11810"
     CDS             1767..2414
                     /locus_tag="VFR42_11810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0369893.1"
                     /translation="MRASSEQAEGVAISDAVAVERTLAGEREAYRVLVERHSQYVYRL
                     AFRMTGNKEDAEEVVQEAFLRAYQKLQQFAGNANFGTWVYRIAANYAIDRLRQRKSED
                     SRREQPKRIAEGEPESDPLSEVKDAAPSPERLAGSAQLAAKMKEALDTLTPAERTAIV
                     MRHWDGCGIEEIAAVLKSNTSAAKNTVFRAVQKLRLALQPFAEPRGAARAVGTES"
     gene            2411..3220
                     /locus_tag="VFR42_11815"
     CDS             2411..3220
                     /locus_tag="VFR42_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369894.1"
                     /translation="MKHLTEEELFTYREGDTKGREAMAAHLKECTDCRAELARLEQVF
                     AALDVMPVPDPGEDYGTRVWRQIAPRLPEKRASWWQTFFTTQRLAVAGAVAALVIMAF
                     YAGKKVGPGAAGGSNGAVVAAASAEKVRERVLILAVGDHLGKSEMVLVELSHASSAAG
                     GKELINISAEQKRAESLVEENRLYRQTALEGGDSAMASTLDELERVLMDVANSPAKVT
                     PAQFESIQKRIAEQGILLKVRVVRQELRTAETGRKSQPAQTDSTPRERNKV"
BASE COUNT          671 a          902 c         1086 g          645 t
ORIGIN      
        1 ttcgtgctgt taatcgccgg ggtgaacgtc gccaatctgt tgctggcgcg atcgaccggt
       61 cgcgcgcgcg aatttgcgat acgcatggcc ctaggtgcgg gtcgcggtcg aatcttgcgt
      121 cagcttctga cggaaagtct cttactcgca gcgattggcg gaggactcgg cgtcttgctg
      181 gccgctcttg gaacgaaggc tgcattgcgt gtgctgccgg aaaccctgcc gcgcgccgcc
      241 gaagtcggaa tcgacgcgcg cgtcctggtt tttgcggcgg gagtatcgtt gcttgccggg
      301 atctgtttcg gattgctgcc cgcgctaaaa atcagtaagt ctggcgcgca aaccgcgctg
      361 aaggagggcg gccgtggcgg cagcgggacc cgccatagag tgcaacgcgc gctggtcgct
      421 gccgaaatgg cgctcgccct agttcttctg attggcgctg gactcatgat tcgcacgctt
      481 ggcgcgctgt ggaacgtcaa ccccgggttc gagccgaaga acgttctcac cttcggattt
      541 gcgcttccct cggcgatgag cggggcgaac gcggattcgg ttcgtgccgc acttcgcgaa
      601 gttcatgaaa agttcgcggc ggctcccgca attcaggcag tttctttttc ctggggagca
      661 cttccgttga gctcggatga tgagtggctg ttctggatcg acggccaagg gaagccggcg
      721 aacgataacg acatgaattg ggcgctgaat tacgtggtcg agccggacta tctgcgggtt
      781 atgggcattc ccctgaagcg cggccgattt ttcaccagcc aggatgacga gcgcggccag
      841 aaagtcgcgg taattgatga agcgctggcc agcaaatttt tctcaggtat cgaccctgta
      901 ggcaagcggt tgcaccttaa caacggcggc gatcagctgg tggaaattgt cggcgtcgtg
      961 ggccacgtga agcagtggag tctggatgcg gaccagaaag agtccctgca agcgcaactc
     1021 tatacgcctt tcatgcagtt gcctgacaaa gcgatggcac aatcggcctc tggaattagt
     1081 gtgctgatac gctcgcaaaa cacggcgtct gttttcgact cggttcgccg agccagcacg
     1141 gaaatgagca accaacaagt cgttttcggc gcgcaaacca tgaacgacat tattgcgaac
     1201 tcattggccg agcggcgatt ctcaatgacc ctgctgggaa ttttcgcggg cgtggcgttg
     1261 gggctcgcca gcatcggcat ctacggagtt gtctcctacc ttgtcgggca gcgcactcag
     1321 gaaatcggcg tgcgcatcgc aatgggagcc cagcgcagcc aagtgctggg cctggtgctg
     1381 gctcagggag cagccatgtc cgccattggg gcggcaattg gcctcggggc cgctcttcca
     1441 ttgacgcgtc tgatgggaac cctgttattt gccgtcagta cagcagatcc tctgacctac
     1501 ctctgcgtgg tggcgattct tctcgtggtt tccatggccg cctgctggct gccggcgcat
     1561 cgggcggcac gcatgaaccc tgtagatgct ttgcgttacg agtaaattcc ctcccggcga
     1621 actttcgatt ggcggcggcc agtccgacaa acgcgataaa cctattcgct tcagggtgat
     1681 accttaccct cggcattgcc gacggctggg ccagcttcct tcgctctgac ctaaccgcaa
     1741 ggggcgtttc ctgtctaatg gtgagaatgc gagcgagcag cgaacaggct gagggcgtgg
     1801 cgatcagcga cgcggtggcg gtggagcgaa cgctcgccgg cgaacgcgaa gcgtatcgcg
     1861 tactcgtgga acgccacagc cagtatgtgt accggctggc atttcgcatg accggcaaca
     1921 aggaagatgc ggaggaagtc gtgcaggaag cttttttgcg cgcctatcaa aagcttcaac
     1981 agttcgccgg caacgcgaat tttggcacct gggtttaccg cattgcggcg aactacgcga
     2041 tcgatcggct gcgccagcgc aagagcgaag attcacggcg cgagcagccc aagcgtattg
     2101 ctgagggtga accggaatcc gacccgctga gcgaagtgaa agacgcggcg ccgtcgccgg
     2161 agcgactggc aggcagcgcg cagttggcgg cgaaaatgaa agaagcgttg gacaccctca
     2221 cgccagccga gcgcaccgca attgttatgc gccactggga cggctgcgga attgaggaaa
     2281 ttgcggcagt gctgaaatcc aacaccagcg cggcaaaaaa caccgtgttt cgcgcggtgc
     2341 agaaattgcg gctggcgctg cagcccttcg ccgaaccacg cggagcggcg cgcgccgtgg
     2401 gaacggaatc atgaaacacc taacggaaga agagttgttt acctaccgag aaggcgacac
     2461 caaaggccgt gaggcgatgg ctgctcattt gaaggagtgt accgactgcc gggccgagct
     2521 ggcacggctg gaacaagttt tcgctgcgct ggatgtgatg ccggtgcccg atcccggcga
     2581 ggactatggc acgcgggttt ggcggcagat tgcgccgcgc ctcccggaaa agcgcgccag
     2641 ctggtggcaa acgttcttca ccacgcaaag actcgcagtg gcaggcgcag tggctgcgtt
     2701 ggtgattatg gcattttatg cgggcaagaa ggtcggaccg ggcgctgctg gtgggagcaa
     2761 cggagccgtt gttgcagcgg ccagcgcgga aaaggtccgt gagcgcgtgc tgattctggc
     2821 ggttggcgat cacctgggca aatccgaaat ggtgctggtg gagctttcgc atgcttcgtc
     2881 cgccgcaggc gggaaagagt tgatcaacat ttccgccgag cagaagcgcg cggaaagttt
     2941 ggtggaagaa aaccgcttgt accggcagac ggcgctcgaa ggcggcgaca gcgccatggc
     3001 cagcacgctc gatgagctgg aacgtgtgtt gatggatgtg gcgaacagcc cggcgaaagt
     3061 caccccggcg cagtttgaat ccatccagaa gcgcatcgcc gagcagggaa ttttgttgaa
     3121 ggtccgggtg gtccggcaag aattgcgaac ggccgaaaca ggaagaaagt cccagcctgc
     3181 gcagaccgat tcgacgccaa gagagaggaa caaagtatga ctgtttgggc taagaacatc
     3241 gcagacggaa tgcgcaaagg cgttattggc ggactggtct ttggactggc ggcgacgccg
     3301 gcat
//
LOCUS       DASTPM010000671         5927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_687525, whole genome shotgun sequence.
ACCESSION   DASTPM010000671 DASTPM010000000
VERSION     DASTPM010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687525"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2298)
                     /locus_tag="VFR42_11820"
     CDS             complement(<1..2298)
                     /locus_tag="VFR42_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0369895.1"
                     /translation="MQQSGPTHTTRWRLGFWSLIVTQFQGAFNDNGLKFFVIYLILGQ
                     NPSETEKDFGVFLIGNLFAIPFLLFSMTGGYLADRFSKRSVTIGTKIFEIAAMLFAIF
                     AFWRGSPYLAFSVIFLASTQAAIFGPSKYGLLPELLPAEELSWGNGVLELTTFLAIIS
                     GTVFGSYLAQTFRGREAIAGVIFAGLTLFGLLMSLLISRVRAGDPTKKFRANIFADLG
                     AQWRLVRPDRALKLAVIGNTYFWFLGALLQFVIVFYGREILHLDETRGSYLQAALAIG
                     IGLGSFAAGYLSGGKIEYGLIPLGGIGMAVFGSLLSFHGLSFAQVMSLLAALGFAGGF
                     FVVPINALIQHRPDEDKKGGVIAYANWLSFVGVLVASVIYSGSTHYLHIGLGTFFIWT
                     ALLSLAVGAYTLWLLPDSLLRLLLWFATHTLYRMDIVGAEKVPTRGGALLVPNHASMA
                     DAALVIAATDRPVRFMMFKDSYEHPIVKPFAKILGVIPISSNQGPREMIHSLREATRA
                     LQNGELVCIFPEGQMTRIGQMMPFRRGMERVLKGLDKDASVPVIPVHIDGIWGSIFSF
                     SGGKFFWKWPRKIPYPVRVTFGEPMPATVTATEARNSVLELGADAFARRKPLMKTLPR
                     HFLQTARRHPFRFAMADFRTAKLNFFSALTKTIFLARRLGPVWAEQKTVGVLLPPSIG
                     GALVNLAAMFAGKVPVNLNYTASQPVVDSCAQQSGLTATITSRAFLEKIKVQPPGKAV
                     YVEDLAEKPGFGEKIVSLLAALFFPA"
     gene            complement(2398..4542)
                     /locus_tag="VFR42_11825"
     CDS             complement(2398..4542)
                     /locus_tag="VFR42_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU0369896.1"
                     /translation="MTRFLVLGLAISLSFAGFTRAQKPPLLPEKEVAALANELSGETA
                     KRNLEGIARFHRQRGSKGFHEAAELVADRLRTYGLSDVAILQFPADGKIFYGTQRSRP
                     AWDAESAELWESSGSAQIKLAGYEADQVVLAEDSESADVTAELVDAGGGTKESDYASK
                     DVRGKLVLVSAQPGAVQDLAVGKFGAAGIISYAQNQPTAWSRENQDAIRWGHLETFSP
                     NKTFAFMVSLKTAQGFQQRLAKGEKIVLRAVVKAGQHPANYEVVTATIPGVAPKLKDE
                     EIAFSCHLDHQRPGANDNASGCSAILEIARTLQKLINEKQLAPAARTIRFIFPPEIEG
                     TMALLTARAEFIAELDPVNGEGRVIPGAEKIHTPINMPLEFPKRIKGVIHMDMVGGGP
                     ETKAVFHVTRGPMSLPSFVHDVAWAFADWLNDQSYQFAATGKAAYPMIAPTGGKEPLR
                     AEYSAFAMGSDHDVYQDSSFAIPAIYMNDWPDRFIHTNFDSAANIDPSKLKRAAFIGA
                     ASGYFLATMNDSSLPAVFRLLQLHTLRVADGISQKRAQIADQCDGGELERFEEQNVRP
                     LVESVVHFGLDSKGDRWLMLGKIGANIANNTEGVCLRALLIVGGNPIFRRKLVPKGPL
                     TVFGYDYFAEHAKSAGITTPKLLSYEGLWGSGEEYAYEVLNFADGNRNAQQIRDSVSA
                     EYGPVPIELVLEYLQALEKIGLVEKVPEQHSK"
     gene            4722..5675
                     /locus_tag="VFR42_11830"
     CDS             4722..5675
                     /locus_tag="VFR42_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0369897.1"
                     /translation="MNRRIFQALAGAVFLAFSWCAISAQEKEPDWSKVQIKVTKVSGN
                     IYMLEGQGGNIAASVGEDGIVIVDDEFAPLAEKIQAALKNLGITDKPVRFVINTHYHG
                     DHSGGNAPFATSGSTLIAQDNVRKRLISGGTAGNGGSMKMETKPAEKAALPIITFEHD
                     VTVHLNGEDIRALHFPAGHTDGDAIIFFPKNNVVHMGDDFVRYGFPFVDVASGGSVQG
                     MIDGVEKAVAQLPADVKVIPGHGVLSNLDDVRTYIQMLKDTTAAVQKAMAAHKSVDQM
                     KQEKLLAPWAKWSNDFVSQDVFIETIYNSLTNHPGPFVKHN"
BASE COUNT         1306 a         1665 c         1788 g         1168 t
ORIGIN      
        1 ggcagggaaa aacagcgccg ccaagagcga aactattttt tcgccgaagc cgggcttttc
       61 ggcaaggtct tcgacgtaga cggccttgcc cggcggctgg actttgattt tttccagaaa
      121 cgcgcgcgag gtaattgtgg cggtcaggcc gctttgctgc gcgcaagaat cgacgaccgg
      181 ctgcgatgcc gtgtagttga gattgacggg aactttgccg gcgaacatgg cggcgagatt
      241 gaccagcgcg cctccgatag aaggcgggag aagaactccg acagttttct gctccgccca
      301 aaccggaccc agacgccgcg ccagaaaaat ggttttggtc aacgcggaga aaaagttgag
      361 cttcgctgta cggaaatcgg ccatggcgaa acggaatgga tgccgccgcg ccgtttgcaa
      421 gaagtggcgc ggaagggtct tcatcagagg cttgcggcgg gcaaaggcgt cggcgccgag
      481 ttcgagtacg gagttgcgag cttcggtggc ggtgaccgtg gcgggcatag gttcaccgaa
      541 cgttacgcgc accgggtacg gaattttgcg cggccacttc cagaaaaatt tgccgccgga
      601 aaaactgaaa atggagcccc agatgccgtc aatgtgaacg gggatgaccg gcacgctggc
      661 gtctttatcg agacccttga ggacgcgttc catcccgcgt cggaacggca tcatctggcc
      721 gatgcgggtc atctggcctt ccggaaaaat gcagaccagc tcgccgtttt gcagcgcgcg
      781 agtagcttcg cgcagcgagt gaatcatctc gcgcgggccc tggttggaag aaatgggaat
      841 gacgccgaga attttggcga acggctttac gataggatgt tcgtaggagt ccttgaacat
      901 catgaagcgg acagggcgat cggtagcggc gatgaccagc gcggcgtcgg ccatggacgc
      961 gtgattggga acgagcaacg cgccgccgcg cgtgggaacc ttttccgcgc cgacgatgtc
     1021 catgcgatac agcgtgtggg tggcgaacca gagcagcagt cggaggagag aatcgggcag
     1081 cagccagagc gtataagcgc ccacagcgag cgacagcaaa gcggtccaaa tgaaaaacgt
     1141 cccaagccca atgtgcaggt aatgcgtgga accggaatag atgacggacg caaccaggac
     1201 cccgacaaaa gacagccagt tggcgtacgc gatgacgccg ccttttttgt cttcgtccgg
     1261 gcgatgctgg atgagtgcgt tgatggggac gacgaagaag ccgccggcaa atcccagcgc
     1321 agcgagcagc gacatcactt gcgcgaacga cagtccgtgg aaagaaagca aacttccaaa
     1381 aacagccatg ccgatgccgc cgagcggaat caggccgtac tcgatttttc cgccggaaag
     1441 gtatcctgcg gcgaagctgc ccagaccgat gccgatggcc agagcggctt gcaggtagct
     1501 accgcgcgtt tcatccagat gaagaatttc gcggccatag aacactatta cgaattgcag
     1561 aagcgcgccg agaaaccaga agtacgtatt tccaatgacg gccagcttta gggcgcggtc
     1621 tggacgcacc agacgccact gggctccgag gtcggcaaaa atgttggccc gaaatttctt
     1681 ggtgggatcg ccggcccgga cgcgagagat cagcaagctc atcagcagcc cgaacagcgt
     1741 gaggccggca aaaatcacgc cggcgattgc ttcgcgtcca cgaaaggttt gcgccaaata
     1801 agatccgaaa accgtgcccg agataatggc gaggaaagtg gtgagttcga ggactccatt
     1861 tccccaggaa agttcttcgg caggcaagag ctcgggcagc aggccgtatt tcgacggacc
     1921 gaaaatcgct gcttgcgtgc tggccagaaa aattacgctg aaagcaagat aagggctgcc
     1981 gcgccagaac gcgaagatgg caaagagcat cgccgcaatt tcaaatatct tcgtaccgat
     2041 ggtgacgctg cgcttactga aacggtcggc caggtagcca ccggtcatcg agaacaatag
     2101 gaagggaata gcgaaaagat tgccgatcag gaaaacacca aaatcctttt cggtttcgga
     2161 cggattttgg cccaggatga gatagatcac gaaaaacttg aggccgttgt cgttaaacgc
     2221 gccctggaac tgggtgacga tcaggctcca gaagccgagg cgccagcgtg tggtgtgtgt
     2281 cggcccgctt tgctgcatgc gcagcccctc cgatgggcat acgtcgctgc gtggatcaga
     2341 atgcgctgaa agtgatcgcg gagattataa cggtggttgt gggctgcgat tcacaaacta
     2401 ttttgaatgt tgctccggca ctttttccac gaggccgatc ttttcgaggg cttggagata
     2461 ttcgaggacc agctcgatcg ggacggggcc gtattcggcg gaaacggaat cgcggatctg
     2521 ctgcgcgttg cgattgccgt cggcaaaatt caggacttcg taggcatatt cttcgccgct
     2581 gccccacagg ccttcgtaac tgagaagttt gggtgtagtg atcccggcgg acttggcatg
     2641 ctcggcgaaa taatcgtagc cgaagaccgt gagcgggcct ttgggcacga gcttgcgcct
     2701 gaagattgga ttgcctccaa caataagaag cgcccgaagg caaacacctt ccgtattatt
     2761 ggcaatattg gcgccgatct ttcctagcat cagccatcgg tctcccttgg aatcgaggcc
     2821 aaagtgcacc actgactcga caagcggccg cacgttctgc tcttcaaatc tttctagttc
     2881 cccaccatcg cactgatcgg cgatctgggc ccgcttttga ctaattccat cggcgacgcg
     2941 aagggtgtgc aactgcaaga gccggaagac tgccggcaga ctgctgtcgt tcatcgtcgc
     3001 caggaagtag ccactggctg cgccgatgaa agccgcgcgc ttgagtttgc tgggatcgat
     3061 gttggcggcg gaatcgaagt tggtatggat gaagcggtcg ggccagtcat tcatataaat
     3121 tgccgggatg gcgaacgacg aatcctgata gacgtcgtgg tcgctgccca tggcgaaagc
     3181 ggaatattcg gcgcggagag gttccttgcc gcctgtggga gcaatcatcg ggtaagcggc
     3241 tttacccgtg gcggcgaact ggtaagactg atcgttgagc caatcggcga aggcccaggc
     3301 gacgtcgtgg acgaaactgg ggagactcat ggggccgcgt gtcacgtgga acactgcctt
     3361 ggtttctggg ccgccgccga ccatgtccat gtgaatgacg cctttgatgc gcttggggaa
     3421 ctccagcggc atgttgatcg gagtgtgaat cttctcggct cccggaataa cccgcccctc
     3481 gccattgacc gggtcaagtt ctgcaataaa ttctgctctt gcggtgagaa gagccatggt
     3541 tccctcgatt tcgggcggaa agatgaagcg gatggtgcgg gcggccggcg cgagctgttt
     3601 ttcgttgatg agtttttgca gcgtgcgggc gatttccagg atggcggaac agccactggc
     3661 gttgtcgttg gcgccgggac gctggtggtc gaggtggcaa ctgaaggcaa tctcctcgtc
     3721 tttcaacttc ggggcgacgc caggaatcgt tgcggtgaca acttcatagt tggcaggatg
     3781 ctggccggcc tttacgacgg cgcgcagaac gattttttcg cctttggcga ggcgctgctg
     3841 aaaaccctgc gccgttttca gagaaaccat gaaggcgaag gttttgttcg gcgagaaagt
     3901 ttccagatgg ccccagcgga tggcgtcctg attttcgcgg gaccacgccg tcggctggtt
     3961 ttgcgcgtag ctgatgatgc ccgccgcgcc gaattttcca acggcgagat cctgaacagc
     4021 gcccggctgc gcactgacga gcacgagctt tcctcttaca tccttgctgg cgtagtcgct
     4081 ctctttcgtg ccaccgccag catccaccag ctccgcggtg acgtcggcgc tctcgctgtc
     4141 ctcggcgaga accacttggt ccgcctcgta gccggcgagt ttgatttgcg cgcttcctga
     4201 cgactcccaa agctcggcag actccgcgtc ccaggcgggg cgggagcgtt gcgtgccata
     4261 aaaaattttg ccgtcggcag ggaactggag gatggcaacg tcgctgaggc cataggtgcg
     4321 caggcggtcg gctacgagtt cggcggcttc gtggaaacct ttcgaaccac gctggcggtg
     4381 aaagcgcgca atgccctcaa ggttgcgctt ggcggtttca ccgctgagct cgttggccag
     4441 cgccgcaacc tctttttcgg gcaggagcgg tggcttttgc gcgcgagtga aacccgcaaa
     4501 actcagggaa attgccaagc caaggacaag gaaccgagtc acgtaaccct cccgaatcgg
     4561 gagggattct ataccgaacg agagcatagt gcagtcaggc ggaatggtag cggtcgcgag
     4621 cagcggccgt ctgcaaactg ctgcgttgga catcgccgaa ggccagaact cactgcggca
     4681 cgagcagtgc tagaatctga ccacttggtc ggagggctcc catgaatcgc aggattttcc
     4741 aggcactggc tggcgcggtg ttcctcgcat tctcctggtg cgctatttcc gctcaggaaa
     4801 aagagcccga ttggtccaag gtccagatca aggtcaccaa agtttccggc aacatctata
     4861 tgctcgaggg ccaaggcggc aacatcgccg caagcgtcgg cgaagacggc atcgtcatcg
     4921 tggacgacga attcgctccg ctggcggaaa aaatccaggc tgcgctcaaa aatcttggca
     4981 tcaccgacaa gcccgtccgc ttcgtcatta acactcacta tcacggtgac cacagcggcg
     5041 gcaacgcgcc attcgccaca tccggttcca ccctcatcgc gcaggacaac gtgcgcaagc
     5101 gcctcatctc cggcggcacc gcaggtaacg gcggctccat gaagatggaa acaaagcccg
     5161 ccgaaaaagc cgctctcccc atcatcacct tcgagcacga cgtcaccgtc cacctcaacg
     5221 gcgaagatat tcgcgcgctc cactttcccg ccggccatac cgacggcgac gccatcatct
     5281 tctttcccaa aaacaacgtc gttcacatgg gcgacgattt cgtccgctac ggctttccct
     5341 tcgttgatgt cgccagcggc ggcagcgttc agggcatgat tgacggcgtc gaaaaagccg
     5401 tcgctcagct gcccgccgac gtaaaggtca tccccggcca cggtgttctc tctaacctcg
     5461 acgacgtccg cacctacatc cagatgctta aagacaccac ggccgccgtt cagaaagcaa
     5521 tggccgctca caaatctgtc gaccaaatga agcaggaaaa acttctcgcg ccctgggcca
     5581 aatggtccaa cgacttcgtc agccaggacg tcttcatcga aaccatctac aactccctaa
     5641 ccaaccaccc cggccccttc gtcaagcaca actgatagct gtcgcgcctc agccccgata
     5701 gttgtcatct gaacccagtg aggggtctgc ttttccgagg gacgaagggc agtcgaggaa
     5761 tctgcttttg ttctttctcg tagtggccgg tcttcagact ggtgcttttg tcagtaacgg
     5821 tcttcatttc cagcttagga tgcaaatagc ggaccgccgc gttttacggc ttcgccgtag
     5881 gcgagaaaaa cccgatcggc aaatccttcg ccgttcgcag accggcc
//
LOCUS       DASTPM010000672         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_168212, whole genome shotgun sequence.
ACCESSION   DASTPM010000672 DASTPM010000000
VERSION     DASTPM010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168212"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..915)
                     /locus_tag="VFR42_11835"
     CDS             complement(34..915)
                     /locus_tag="VFR42_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0369898.1"
                     /translation="MKTLLLRTIGLLVFLAPCAIAQDVIPLYAGTPPGSTQENYPEKE
                     YFSKSWNTEVVANVTKPTLTVFKPAPELRNGTAVVICPGGGFMALSINSEGNDVAKYL
                     TAKGVTAFVLKYRLAHTGEDATQEFAALFEDRPKFRAILDKIVPLTIADGLAAVSYVR
                     QHAADWKVSPDRVGIIGFSAGGTVAAGVAFGYAPEGRPAFAGLVYAAGERFKDSAVPA
                     DAPPVFVIAATDDNLGLAPDSVALYEKWANAHKPAELHMYVKGGHGFGMHKQNLPTDH
                     WIDRFAEWLELEGWLKK"
     gene            complement(967..1902)
                     /locus_tag="VFR42_11840"
     CDS             complement(967..1902)
                     /locus_tag="VFR42_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0369899.1"
                     /translation="MNRREFLYGAAAIVSIKGAPLAREQSRAPEIAFTFDDPTTNGGA
                     NRTWNEVNERILAALANHRIKAALFVCGKRVDSSAGRQLITAWDQQGHAIANHSYSHL
                     FFNNDEKVTLAEFEADALKNEPLIRDYRHFTKLFRFPFFKEGDTAEKRDGMRSFLRER
                     GYRIGRATIDASDWAISTRLQKRAEENRQADLAGYRDFFLQHIWERAQFYDSLAQRLY
                     PQPVRHTVLLHHNPLNAFFLDELIGMFEKRGWKPIDAEYAYKDSVYDRQPKILPAGES
                     LIWALAKETGKFENELRYPGEDDVYENPRMDALKL"
BASE COUNT          419 a          672 c          619 g          525 t
ORIGIN      
        1 cattgattcg cggctcgctc tatttcttga gattcatttc ttcagccagc cttcgagctc
       61 cagccattcc gcaaaacggt cgatccagtg atccgtcggc aggttttgtt tgtgcattcc
      121 gaacccgtgg ccacccttca cgtacatgtg cagctccgcc ggtttgtgcg cgttggccca
      181 tttttcgtac agcgcgacgc tgtccggcgc cagcccgaga ttgtcatcgg tggccgcaat
      241 gacgaacacc ggtggcgcgt ccgccggaac cgcagaatcc ttgaaccgtt cccccgcggc
      301 ataaaccagg ccggcgaagg ccggccttcc ttccggcgca tatccgaagg caactcccgc
      361 cgcgactgtt cctccggccg agaatccgat aatgcccacg cgatccggag aaactttcca
      421 gtccgcggcg tgctgccgga cgtagcttac cgccgccaac ccatccgcga tggtcagcgg
      481 aacaatcttg tccaggatgg cgcggaattt cggccggtct tcaaacagcg ccgcaaattc
      541 ctgtgtggca tcttcgcctg tatgggccag ccggtatttc aatacgaaag cggtcacccc
      601 tttcgcggtc aggtatttgg ccacgtcgtt gccctcgctg ttgatcgaga gcgccataaa
      661 acctccgccc gggcagatca ccacggcggt gccatttctc agctccggcg ctggcttgaa
      721 caccgtcagc gtcggcttgg tcacgttggc caccacttcc gtgttccagc tctttgaaaa
      781 atattctttt tccggatagt tttcctgtgt cgagcccggc ggcgtgcccg cgtaaagggg
      841 aatgacgtcc tgggcgattg cgcagggcgc caggaatacc agcaggccaa tggtgcgaag
      901 tagaagtgtt ttcatggccg cagagtctag cagagagtcg cctggattgg gctctttatc
      961 ctgcaattac agcttcagcg catccattcg cggattttca taaacgtcgt cctcgcccgg
     1021 atagcgaagc tcgttctcaa attttccggt ttcttttgcc agcgcccaga tcaggctttc
     1081 tcccgccggc aagatcttcg gctggcggtc ataaacggaa tctttgtacg catattccgc
     1141 gtcaatcggc ttccagcctc ttttttcaaa catgccaatg agttcgtcca ggaaaaaagc
     1201 attcagcgga ttatggtgca gcagcacggt atgccgcacc ggctggggat aaagacgctg
     1261 cgccagcgag tcgtaaaatt gcgcgcgttc ccagatgtgc tggaggaaaa aatcccgata
     1321 gccggccagg tccgcctggc gattttcctc cgcgcgcttc tgcagccgcg tgctgatcgc
     1381 ccagtcagaa gcatcgatcg tggcccgccc gatgcggtag ccgcgctcgc gcaagaacga
     1441 gcgcatgcca tcgcgcttct ccgccgtatc tccctccttg aagaacggaa agcgaaacag
     1501 cttggtgaag tgacggtagt cgcgaatgag tggttcattc ttcagcgcgt ccgcctcgaa
     1561 ctccgcgagc gtgacctttt catcgttatt gaaaaaaaga tgcgagtacg aatggttggc
     1621 aatggcgtgt ccttgctggt cccaggcggt tatcaattgg cgccccgcgc tgctgtccac
     1681 gcgcttgccg cacacaaaaa gcgccgcttt gatcctgtgg ttcgcaagag cggcaagaat
     1741 gcgctcgttt acctcgttcc atgtccgatt ggcgccgccg ttcgttgtcg ggtcgtcaaa
     1801 ggtgaaagcg atttcgggcg cgcggctctg ttcccgggcc aacggtgcgc ctttgattga
     1861 aacgattgcc gctgcgccgt aaagaaattc cctgcgattc atcaagtgga gcttatccca
     1921 tccgaccgcc agccccaaaa ataagagcaa gcacgcaaac cgcaatcacg cccatcgcgc
     1981 gggtaacttg tcgcttggct cggttgcttg ctctgatata ctccacggca ttcgggcatt
     2041 ttcaggtttt gggggtggag cgtcatgaag cgttttctgt ctggtgtgtg ttttgcgttt
     2101 ttgctgccgt gtctattcct gttgctttct tctttggcac ctgctgcgcg cgggcaggct
     2161 atcaccgcga cactttccgg aagagtgctg gatacttcgg gcggcagcgt ggccaaggcc
     2221 aaagtgacca tcgcc
//
LOCUS       DASTPM010000673         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_946920, whole genome shotgun sequence.
ACCESSION   DASTPM010000673 DASTPM010000000
VERSION     DASTPM010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946920"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFR42_11845"
     CDS             complement(<1..250)
                     /locus_tag="VFR42_11845"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0369900.1"
                     /translation="MTRQTKKEEAGFVRAIGLFDGTMIVVGSMIGSGIFIVAADITRQ
                     TGSPGGLLLTWILTGLLTIAAALTYGELAALFPHAGGQY"
     gene            complement(284..934)
                     /gene="thiE"
                     /locus_tag="VFR42_11850"
     CDS             complement(284..934)
                     /gene="thiE"
                     /locus_tag="VFR42_11850"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0369901.1"
                     /translation="MANRLALPRLYVILVAALLPSDLVEFEKQLMGAGARLFQYRNKT
                     APAREVLHASQALGVTARQEGSSFVVNDRPDIARLAWANGVHLGQDDLDVAKARAIVG
                     SDAIVGISTHNLEQFKAAADTDADYIAVGPVFETRSKEKPDPVVGLELIREARKLTSK
                     PIVAIGGITLERAAEVIAAGADSVAVISDILAAKNPAARVKQYLEILPAAAQPAKN"
     gene            complement(935..2287)
                     /gene="accC"
                     /locus_tag="VFR42_11855"
     CDS             complement(935..2287)
                     /gene="accC"
                     /locus_tag="VFR42_11855"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU0369902.1"
                     /translation="MFNKILVANRGEIALRVICACKELGIATVAVYSEADRNSLHVRF
                     ADEAVCIGPPRSSESYLNIPQVISAAEITNVDAIHPGYGFLSENANFAKVCEASEITF
                     IGPRPEIIEMMGEKDRARREVKHAGLPTIPGSDGAIEGEEQLSKEAAKIGYPLILKAV
                     AGGGGRGMRVVRKQEELLSAYETARSEAQQAFGTPDVYAERFIEHPRHIEFQVLGDQH
                     GKVIHLGERECSIQRRHQKLIEESPSPVLDPKVRKEMGSKVVRALQKIGYTNAGTVEF
                     LMDQDGSIYFIEMNTRIQVEHPVTEFVTGADLIKAQIRIAAGEKLDDAVGEIHFSGHA
                     IECRINAEDPETFVPSAGRITTFQAPGGTGVRVDSAAYADAVIPPYYDSMIAKLIVKG
                     RDRAEAIGRMKRALEMFVIEGIKTSIPLHRRILADPDFAAGKFDTHFVERFLGNNGKH
                     "
     gene            complement(2303..2839)
                     /gene="accB"
                     /locus_tag="VFR42_11860"
     CDS             complement(2303..2839)
                     /gene="accB"
                     /locus_tag="VFR42_11860"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU0369903.1"
                     /translation="MKRKKPSKPSGRKAVANPEVEQIEHLLKTMAEHNLEEFEYSRGD
                     LRIRLKKPSAGVAYLPAQTPAPEIIVTGAPAEAASSISSAAAAAPEARASEDLHLVKS
                     PIVGTFYASPSPGAEPFVKIGGYIEAGQTLCIVEAMKLMNEIESDMSGEVLRIFAENG
                     QPVEYGQPLFGIRPSRKK"
     gene            complement(2888..>3435)
                     /locus_tag="VFR42_11865"
     CDS             complement(2888..>3435)
                     /locus_tag="VFR42_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU0369904.1"
                     /translation="THGASGPAFPTIVAFGARSALPHARPTAKRLKKGQLVVLDLGAI
                     LNRYCSDITRTVFVGKAPAKVRLWYQAVLEAQAAAIAAVAAGRTCGDVDAAARGVLAA
                     YRLDQYFVHSTGHGLGLEVHENPRVAKGQKTLLASGNVVTIEPGVYVPGVGGIRIEDD
                     VAVHQSGNEVLTRASREFIEL"
BASE COUNT          647 a         1140 c          895 g          753 t
ORIGIN      
        1 catactgtcc gcccgcatgg gggaacagcg ccgccagttc gccataagta agcgcagcgg
       61 caatcgtaag caatcccgtc agaatccacg tcagcagcag cccgccgggt gaaccggttt
      121 gccgcgtgat gtcggcagcg acgataaaaa tccccgaccc gatcatcgag ccgaccacaa
      181 tcatcgtgcc gtcgaacaat ccgatggcgc gaacgaaacc tgcttcttcc tttttagtct
      241 gcctagtcac tggaagagcg gtttcggcca taagcaggtc gcctcagttc tttgcgggct
      301 gtgccgcagc gggaagaatt tccaaatact gtttgacgcg cgccgccgga tttttggctg
      361 ccagaatgtc gctgatcacc gccacgctgt cggccccggc tgcaatcact tctgccgctc
      421 gttctagcgt aatcccgcct attgccacaa tgggcttgct tgtcagtttg cgtgcctcac
      481 ggattaactc cagtcccacc accggatcgg gcttctcttt gctgcgcgtt tcaaacaccg
      541 gcccgaccgc aatgtagtcg gcatccgtgt ccgccgcagc cttaaactgc tcgagattgt
      601 gcgtggaaat gcctacgatt gcatcggaac ccacgattgc acgcgccttg gccacatcca
      661 gatcgtcttg gccaaggtgt actccgttgg cccacgccag ccgcgcaatg tccggccggt
      721 cattcaccac aaagctgctg ccttcttgcc gcgctgttac acccagggct tgcgatgcat
      781 gcagtacttc gcgcgccggg gcagtcttgt tgcgatactg aaaaaggcgc gctcccgccc
      841 ccatcaattg cttctcaaat tccactaaat cgctgggtag caacgctgcg actagtatca
      901 cgtaaagccg cggcagggca aggcgattcg ccatttaatg ctttccgttg ttgcctaaaa
      961 accgctcgac gaagtgggtg tcgaatttgc cggcggcgaa atccggatca gccagaatcc
     1021 gccggtgcag cggaatcgac gtcttgatgc cttcgatgac gaacatttcc agcgctcgct
     1081 tcatccgccc gatggcttcc gcacggtcgc ggcccttcac gatcagcttc gcaatcatcg
     1141 aatcgtagta cggcggaatc accgcatcgg cataagcggc cgaatccacg cgcacgcctg
     1201 tcccgcccgg cgcctggaac gtggtgatgc gtcccgcgga cggcacaaac gtttccggat
     1261 cttcagcatt gatgcggcat tcaatggcgt ggccgctgaa gtgaatttcg ccaaccgcgt
     1321 cgtccagctt ttcgcccgcc gcgatccgga tctgcgcttt gatcaggtct gctccggtca
     1381 caaactcagt caccggatgt tctacctgga tacgcgtgtt catctcgata aaataaatcg
     1441 agccatcctg atccatcagg aattccactg tgccggcgtt ggtgtagccg attttctgca
     1501 gcgctctcac taccttgctg cccatttcct tgcgaacctt cggatcgagg accggcgacg
     1561 gcgattcttc gattagcttc tgatggcgcc gctgaatgga gcattcgcgt tcgcccaggt
     1621 gaatcacttt gccgtgctgg tcgcccagca cttggaactc aatgtgccgt gggtgttcga
     1681 tgaaacgctc ggcataaaca tcgggcgtcc cgaaagcctg ctgcgcttcg ctgcgcgctg
     1741 tttcgtacgc cgacagcaac tcttcctgct tgcgcaccac gcgcatgccc cgcccgcccc
     1801 cgcccgcgac tgccttcaaa atcagtgggt agccaatctt ggcggcttcc ttgcttaatt
     1861 gctcttcccc ttcaatcgcg ccatcgctcc ctggaatcgt cggcagaccc gcatgtttca
     1921 cttcccggcg cgcgcggtct ttctcgccca tcatttcgat aatttccggc cgcggcccga
     1981 taaacgtaat ctccgacgcc tcgcaaacct tcgcgaagtt ggcgttttcc gataaaaatc
     2041 catagccggg atggatggca tccacgttgg tgatctccgc ggcgctgatg acctgcggaa
     2101 tgttcaggta actttcgctg ctgcgcggcg gcccgatgca caccgcttca tcggcaaaac
     2161 gcacatgcag cgagttgcgg tcggcctccg aatacacggc taccgtcgca atccccagct
     2221 ctttacaggc acagatgacc cgcagggcga tctccccgcg gttagccacc agaatcttat
     2281 tgaacatagt tcaggtcgcc gcctacttct tgcggctcgg tcggattcca aacaacggct
     2341 gcccgtactc caccggctgc ccgttttccg cgaaaattcg cagcacttct ccgctcatgt
     2401 cggactcaat ctcgttcatc agtttcatgg cttccacgat gcacagcgtc tgtccggctt
     2461 cgatatagcc gcctatcttc acaaacggct cggcgcccgg actcggcgaa gcatagaacg
     2521 tacccacgat cggcgacttc accaggtgca ggtcttcgct agcccgagct tcgggcgccg
     2581 ccgccgcagc cgacgagatg ctggacgctg cctcggcggg cgctcctgtt acgatgattt
     2641 cgggagcagg tgtctgcgcc ggaaggtaag ctacgcccgc cgacggcttc ttcagccgga
     2701 tccgcagatc gcctctggaa tactcaaact cttccagatt gtgctccgcc atggtcttca
     2761 gcaggtgctc aatctgttcc acttccgggt tggcaaccgc tttacgcccg ctgggcttcg
     2821 aaggcttttt ccgtttcatt ttcacctcag ggtcctgaaa ccaaccctga taccgccaat
     2881 tttggaatta gagctcgatg aattcccgcg aggcgcgggt caacacttcg ttgccactct
     2941 ggtgcaccgc gacgtcgtcc tcgatccgga tacccccaac gcccggtacg tacacacccg
     3001 gttctatcgt aaccacattt ccagaggcaa gcagtgtttt ttgccccttg gccacgcgcg
     3061 gattctcatg gacctccaag cccagcccat gccccgtact gtgcacgaaa tactgatcca
     3121 gccggtatgc cgccagaacc cctctggctg ccgcgtccac gtccccgcag gtccgtcccg
     3181 cagccaccgc agcgatggcg gccgcctgcg cttccaggac cgcctgatac cacaaccgaa
     3241 ccttcgcggg cgctttcccc acaaagaccg ttcgcgtgat gtcgctgcag tatctattga
     3301 gtatagcacc caggtctagc accaccaact gccctttctt caagcgttta gccgtaggcc
     3361 gggcatgcgg cagagccgag cgggcgccga aggccacgat cgttggaaag gccggaccgg
     3421 aggcaccgtg agtcc
//
LOCUS       DASTPM010000674         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_889222, whole genome shotgun sequence.
ACCESSION   DASTPM010000674 DASTPM010000000
VERSION     DASTPM010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889222"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..123)
                     /locus_tag="VFR42_11870"
     tRNA            complement(47..123)
                     /locus_tag="VFR42_11870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(87..89),aa:Val,seq:tac)
     gene            269..1129
                     /locus_tag="VFR42_11875"
     CDS             269..1129
                     /locus_tag="VFR42_11875"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU0369905.1"
                     /translation="MRRLIINADDFGLTSGVNRAIVEARLQGIVTSTTLMANSLAFEE
                     ATRLAQADPNWSVGCHVVLLDGKPLMEASRIPSLVLPKNGNAFRDGVAGFALRALAGR
                     IDSDQVEAEATAQIRKLQSLGVSVSHVDTHKHTHIFPAALNGILRAARACGVSAIRNP
                     FEVVAANFARAQSGLWKRYAQVKTLRGFAGSFRRAVQKAGFKTPDGTLGIVATGHLNA
                     NLFREIADNMPEGTWELVCHPGYVDADLGTIRTRLRKQREEELAVLTSAAARDALEAR
                     GIRLISYNEL"
     gene            1233..1910
                     /locus_tag="VFR42_11880"
     CDS             1233..1910
                     /locus_tag="VFR42_11880"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0369906.1"
                     /translation="MSEFRPDPRVNTDLSVRVWGMAANGQTFSQHVRARNISISGALF
                     SGLENELKVGDVIGVQYNDRKSRCRVVWVMDAGPVQKMQVGVQIVTDQDCPWKSELRP
                     DQITPPAEPSNRRRFARHRISFPLELRDERVKTPMRVNATDVSGNGCYVETILPLALG
                     TTLRVEFWLGDEKISTTAVVRTSDPGVGNGIEFTGLTPDTKSRLQNHLDKVDPLRANV
                     FERTPQP"
     gene            2070..>2275
                     /locus_tag="VFR42_11885"
     CDS             2070..>2275
                     /locus_tag="VFR42_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369907.1"
                     /translation="MKIGITCYPTYGGSGVVATELGLELAQRGHDIHFISYAQPIRLT
                     EPQPHIHFHEVEVSRYPLFDYPPY"
BASE COUNT          488 a          663 c          668 g          456 t
ORIGIN      
        1 atgaacctgg gaaagggtta gcgaaacatt attgaagaga cagaattggt gggcagtgca
       61 ggactcgaac ctgcgacctc ctgcttgtaa ggcaggcgct ctaaccagct gagctaaccg
      121 cccttcgcga tgaatcgaag agcgccggaa ttgcgcggat acaccgctca gaatgcggcc
      181 aggtcgagta tacgcggcgc tgaaaagcag ggtcaaacct gtcgcggaca ctcggcctca
      241 tgccacggcc cgctgctaca ctcttttcgt gcgccgactc atcatcaacg cggatgattt
      301 cggactgacc tcgggcgtga atcgcgcgat tgtcgaggct cgcctgcaag gcatcgttac
      361 ttcgaccaca ctgatggcga attcgctcgc gttcgaggaa gccacgcgac tggcgcaagc
      421 ggatcccaac tggagcgttg gctgtcatgt ggttctactg gacggcaagc cgctgatgga
      481 ggcctcgcga atccccagcc tcgtgcttcc aaagaacggc aacgcgtttc gcgatggagt
      541 cgctggattc gccttgcgcg cgctcgcggg acgaattgat tccgaccagg tggaagctga
      601 agccaccgcg cagatccgca agctgcaatc tctcggtgtc agtgtttcgc acgtcgatac
      661 acataaacat acccacattt ttccggcagc cttgaacgga attttacggg cggcgcgcgc
      721 ttgcggcgtc tccgcgatcc ggaatccatt cgaagtggtg gcggcgaatt tcgcacgcgc
      781 acagagtgga ttatggaagc gctacgccca agtgaaaacc ctccgtggat ttgcgggaag
      841 ctttcgcagg gcggtccaaa aggctggctt taaaacgccg gacggcacgc tgggcatcgt
      901 cgcgaccggg catctcaatg ccaatttgtt tcgcgaaatc gcggataaca tgccggaggg
      961 tacctgggaa cttgtctgcc atcccggcta cgtggacgcg gatctcggaa cgattcgcac
     1021 gcgtctgcgc aagcagcgcg aggaggaact ggcggtgctg acctctgccg cagcacggga
     1081 tgcgctggaa gcgcgcggca ttcgcttgat ttcctacaac gaactctgag gcgctgcact
     1141 cttcatggca ggcaacaact gtaacttccg taaccgccac tgagccaaag tagggctatg
     1201 atggggggaa gcgagcacat ggcgcggtca ctatgagcga atttcgacca gatccacggg
     1261 tcaataccga tctttcggtt cgggtatggg gaatggctgc gaacggccag acattctcgc
     1321 agcatgttcg cgcgcgcaac atcagcatct cgggcgctct tttttccgga ctggagaacg
     1381 aattgaaggt gggcgacgtg atcggtgtgc agtataacga tcgcaaatcg cgctgccgcg
     1441 tggtctgggt catggacgcg ggtccagttc agaagatgca agtgggcgta cagattgtta
     1501 ccgatcagga ttgtccgtgg aagagtgagc ttcgcccgga tcaaatcacg ccgccagcgg
     1561 aacccagcaa tcggcgccgt ttcgcccgcc atcgcatttc gtttccgctg gaattgcgcg
     1621 acgagcgcgt aaagactccc atgcgcgtga atgcgacaga tgtcagcggg aatggctgtt
     1681 atgtggaaac cattctgccg cttgcgttgg gaaccacatt gcgcgttgag ttctggctgg
     1741 gtgacgaaaa aatctccacg actgctgtgg tacgcaccag cgatcccggc gtgggcaacg
     1801 gcattgagtt cactggcttg acgccggaca cgaagtcgcg tttgcagaat cacctcgata
     1861 aagtcgatcc tttgcgtgcg aacgtattcg agcgcactcc ccagccgtag cgcattcctt
     1921 tcaccgccga acgcgccgag acaaacctcg gactctgctc accaaattaa aatcttggcc
     1981 agttgtctcg gcggtctcgc cattccgccc aaaatctgaa tcctccgtgt aaactcgcca
     2041 tcgaaggaac aggcccggag acgcgctgaa tgaagatcgg gatcacgtgc tatcccacct
     2101 acggcggaag cggagtggtt gcaaccgaac tcggcctcga gctggcgcag cgcggccacg
     2161 acattcattt catctcctac gcgcagccca tccggctcac ggagccgcag ccgcacattc
     2221 acttccacga ggtcgaagtt tcccgttatc cgctcttcga ctaccccccg tacga
//
LOCUS       DASTPM010000675         7272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_254760, whole genome shotgun sequence.
ACCESSION   DASTPM010000675 DASTPM010000000
VERSION     DASTPM010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254760"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..713)
                     /locus_tag="VFR42_11890"
     CDS             complement(168..713)
                     /locus_tag="VFR42_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU0369908.1"
                     /translation="MRKVVYGVGISLDGYIARPDGSVDFLFMPKDYSMGPFFKRVDTA
                     VMGRKTYEVGLKMSGGSEFNGYGLKCFVFSTTRRPGARGGVIFVNTPLKEWLAPLQKQ
                     KGKDIWLMGGGELMRAFLQDDLVDELYLGVVPTLIGEGIPAFPNAFPQREFALVENKS
                     FSKGLIALRYERLRKRRRKAA"
     gene            complement(728..1939)
                     /locus_tag="VFR42_11895"
     CDS             complement(728..1939)
                     /locus_tag="VFR42_11895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0369909.1"
                     /translation="MNKLKMVLLVGVVALVSFCGCKKEVPGEKTEWPGKEWAISTPAN
                     VGLDENTLNALDADLRSGKYPLVDSFAVFRCGKEVFARKYEHDYEKIYGKQAKERGPL
                     NARLTGPYNYFDPAWHPYYHGTGLHSMQSVSKTVSSVIIGIAMQRGDFKADLDTPVLK
                     YFDVAKVKNVDDRKRRMTLRHVMTMTAGLAWTEDVPYDDPRSDSSLMEATDDWVQYVI
                     DKPMTAEPGKVFNYSSGVSELLAHIFLKETGQDFDSYGEKYLFQPLGIEHFWKKTYLG
                     VVDTEGGLYLRGEDLAKIGYLYLHGGNWNGKQMISKDWVTDSVTPFIQAEEDYKYGFK
                     WWLLPRKDSHELAWMARGFGGQNLLVFPREGLIATFTAWDILGTTDPEHEFVARVLSA
                     VRAQSCTANAN"
     gene            complement(1936..2064)
                     /locus_tag="VFR42_11900"
     CDS             complement(1936..2064)
                     /locus_tag="VFR42_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369910.1"
                     /translation="MVRPPNDRPVFVGRGKVLLVLYREADGNWKVKREMWNQGMKP"
     gene            complement(2074..2442)
                     /locus_tag="VFR42_11905"
     CDS             complement(2074..2442)
                     /locus_tag="VFR42_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369911.1"
                     /translation="MKTWTVTCAALILFGETVAASAPRSAEVPAQTASAGDLRKEIDA
                     FNARFLEAHRKMDDAAILGMWAEEGMNLLPETAPMVGKAAITKFLTGVTGRLKGWRME
                     KMDLDFQGIETSGDWASESA"
     gene            complement(2502..5507)
                     /locus_tag="VFR42_11910"
     CDS             complement(2502..5507)
                     /locus_tag="VFR42_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369912.1"
                     /translation="MSSLPLNERDFSKLLLLAAGTMTDTNGAANFTQQFSVNGQRGSA
                     TVFALDGADTTDPELGGATFANFNVDAIQEVQSNSGVLTADIGHGAAGFTNVISKSGG
                     EQVHGSAFEFVRNAAFDARNFFDHASALDPRRIPPFARNEFGVTNGGPVVLPKLYNGR
                     GRTYYFGEYQGFRQVLGTTQVLAVPTATERAGLDTTAFPGETLTVPVSPAVKAILAGY
                     PMPNEPQGAFGERTYATSSKVRTNTDQFSIRIDHHFSETAALMGRFSLNQVDGPINNP
                     DQTAINPTFGVKFFDHQRSATVRFTKAITPRLSSTTQFGYIRSTPFFPAENHTQPAIT
                     FNDGLYAGYNNPDGSIFGSFGNLYQAKEDMTFTRGTHALKWGVEIRLNKDATIFGTNP
                     NGLYAFGGGTAYAPVLITSASGQHDILPGQPLPDALTGFLTATPFSYTITAAANVTPA
                     GDKFDEASVRREAYDFYFMDAWKVNARLKINYGLRFELNSRIKESKKRTSIAIPLDSN
                     GHETSFLTPGASQIFVYNPQPVYPLDKNGWGPRVSVDFAATKHMMLHAGAALTTLLPN
                     LWLQNFVTGGFPLTFQPVVTAKPGAPVPFTDAVVPVTLPDPYTTSGQLLFPNGDSSRV
                     PANTVIDLQRYQNDLAALTPGNEVQLFQPGIISRNFKNGYLGTYTAGIDQDLAGMKLT
                     AAYVGLSGVRLPNGWFPNGFAGASPGFAPFTKFDSNGNVLGGFGPEAVMTNGSHSTYH
                     ALQTGATMNQSKIGMAFQASYTFSKSIDNTSAILGGPAGNTGVILQTVPQDPLDMDGE
                     RGPSTFDATHVFTLSVFQALPLDKVGFLRPVSKKLTGGWQILNITTLTSGSPFTVFSG
                     VQQTGVGAAGADRPDQVGKPNFSTNRRVREDYFGLGTNNSSFFSIPIGVAGGTGPNSG
                     RFGTLGRDTFRGPGFHNFDFSVIKDTSFGSRGKNELCILQFRAEFFNIFNIVNFGLPN
                     NVVRGSGFGIVNKTAGTSRQIQFSLKLIY"
     gene            complement(5738..5902)
                     /locus_tag="VFR42_11915"
     CDS             complement(5738..5902)
                     /locus_tag="VFR42_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369913.1"
                     /translation="MAWYAMLRAKRWPGRQLNFAQAAPRRSNTKQLPVRMENSRLPAF
                     SQETMTLLFE"
     gene            complement(6155..7189)
                     /locus_tag="VFR42_11920"
     CDS             complement(6155..7189)
                     /locus_tag="VFR42_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEU0369914.1"
                     /translation="MATSKVAISPMKAAQISKPGGDFEIVEREIPKPGAGQVRIKVQA
                     CGVCHSDVLTKEGAWPGIQYPRVPGHEVAGVIDEVGASVTEWKQGQRVGVGWHGGQDG
                     TCLSCRRGDFRNCKNLKIAGISYDGGYQQYMVAPVEALAAIPDTLSDVEAAPLLCAGI
                     TTYNALRHSGATPGDLVAVQGIGGLGHLGVQFANKFGYRVAAIGRGAENGVLAKKLGA
                     NVYIDSKATNAAEELQKLGGAQVILATAPSSKAMSELIDGLGPNGKLMVIGAAFDPIE
                     VTPIQLISGSRSIQGWASGTAADSEDTLRFAELSGVRAMIETYPLEKAAEAYARMLSG
                     NAQFRVVLTM"
BASE COUNT         1492 a         2412 c         1717 g         1651 t
ORIGIN      
        1 atctgaaacg cgccgcgtcg tcaaacaggg taaaagggaa gcgaagaagg ccgtcaagtc
       61 agcgaagaag gtcgctgcat ccgccaagaa atccatcaag cgtgtcgcca agaagcttaa
      121 gcgccgctag gaaattgccg cgccgcgatt tttgggccgc cggttcgcta cgccgccttg
      181 cgcctccttt tccgcaagcg ctcataccgc agcgcaatca atcctttcga aaacgacttg
      241 ttctccacca gcgcgaactc ccgctgcgga aaagcatttg gaaacgcggg aattccttcg
      301 ccgatcagcg tcggcaccac gcccaggtaa agttcgtcca ccaggtcgtc ttgcaaaaat
      361 gcgcgcatca actcaccgcc gcccatcagc cagatgtctt tgcccttttg cttttgcaat
      421 ggagccagcc actctttgag aggtgtgtta acaaagataa cgccgcctct cgctccaggc
      481 cggcgagtgg tcgaaaagac aaaacatttc aatccgtatc cgttgaactc ggaacctccg
      541 ctcattttta agccgacctc ataggttttg cgccccatca cggctgtgtc cacgcgcttg
      601 aagaatggcc ccatcgaata gtctttcggc ataaacaaaa agtccaccga cccgtccggc
      661 cgcgcgatgt acccgtccag actgattccc actccgtata ccacttttcg cacggttgcc
      721 tccgatctca gttggcgttg gctgtgcagc tttgtgctcg cacggccgac agcacccgcg
      781 ccacaaattc atgttcagga tccgttgtac ccagaatgtc ccatgcggta aaggtcgcga
      841 tcagcccttc tcgtggaaac acaagcagat tctgcccgcc gaaccctctc gccatccatg
      901 ccagctcatg cgaatccttc cgcggcagta accaccactt gaacccgtac ttgtaatctt
      961 cctccgcctg gataaacggc gtaaccgaat cggtcaccca atccttcgag atcatctgct
     1021 ttccgttcca gtttccgcca tgtagataca aatacccgat ctttgctaga tcctcgccgc
     1081 gcaggtacag tccgccttcg gtatccacca cacccagata cgttttcttc cagaaatgtt
     1141 caatcccaag tggttgaaat aggtacttct cgccatacga atcaaagtcc tgccccgtct
     1201 ccttcaaaaa aatatgcgcc agcagctccg aaaccccgct gctgtaattg aagaccttcc
     1261 cgggttccgc cgtcatcggc ttgtcgatca catactgcac ccagtcgtcc gtcgcttcca
     1321 tcaagctcga atcgctccgc ggatcgtcat acggcacgtc ctccgtccac gccaagcctg
     1381 ctgtcatcgt catcacatgc cgcagcgtca tccgccgttt acgatcatcc acgttcttca
     1441 ctttcgccac gtcaaaatat ttcagcactg gtgtatccag gtcggccttg aaatcgccac
     1501 gctgcattgc aattccgata atcacggaac tcaccgtctt gcttaccgac tgcatgctgt
     1561 gcagtcctgt tccgtgataa tacgggtgcc acgccggatc aaaatagttg tatggccccg
     1621 tcagccgcgc gttcagcggc ccccgctcct tcgcctgctt cccgtaaatt ttctcgtagt
     1681 cgtgttcgta cttcctcgcg aacacctctt tcccgcaacg aaaaaccgca aagctgtcca
     1741 ccaggggata tttcccgctc ctcaaatccg cgtccagcgc gttcaacgtg ttctcatcga
     1801 gtccaacgtt ggccggcgtg cttatcgccc actcttttcc tggccactcc gtcttctccc
     1861 caggtacttc tttcttgcat ccgcaaaagc tcaccaacgc aaccacgccc accagcaaca
     1921 ccatcttcaa tttgttcaag gcttcatccc ctgattccac atttcacgct ttaccttcca
     1981 atttccatcc gcttcacggt acagcaccag caacaccttc ccgcgcccga caaatacggg
     2041 ccggtcattg ggcggccgca ccacctgatg ctcctatgcc gattccgagg cccagtctcc
     2101 gcttgtttct ataccctgaa aatccaggtc catcttttcc atccgccagc ccttcaaccg
     2161 tccggtcact ccagtcagaa atttcgtgat cgccgcctta cccaccatcg gcgccgtctc
     2221 cggcagcaag ttcattccct cctccgccca catccccaga atcgccgcgt cgtccatctt
     2281 ccggtgcgcc tcaagaaatc tcgcattgaa cgcgtcaatc tccttgcgca ggtcccctgc
     2341 cgaggccgtc tgcgcaggga cctcagcact gcgtggcgca gacgccgcca ccgtttcccc
     2401 gaatagaatc agcgccgcgc acgtcacagt ccaagtcttc atgattccgc ctgtcaggct
     2461 ttctctgcgc actctgggtc tctgcgtttc gtggtctcgt cttaataaat caatttcaac
     2521 gaaaattgaa tctgtcgcga agtccccgcc gttttgttga caatcccgaa cccgctccca
     2581 cgcaccacat tgtttggcaa tccaaaattt acgatattga aaatattgaa aaactccgct
     2641 cggaactgca ggatgcacaa ttcattcttg ccgcggctgc cgaatgacgt gtccttgatc
     2701 acactgaagt caaagttgtg gaaacccggc ccgcgaaagg tatcccgccc cagcgtcccg
     2761 aatctgccgc tgtttggccc cgttccgccg gccaccccaa taggaatact aaaaaaagag
     2821 ctattattcg tccccaaccc aaagtagtct tcgcgcaccc tccggttcgt cgaaaaattc
     2881 ggcttgccga cctgatccgg ccggtccgcc cccgccgcgc caactcctgt ttgttgtact
     2941 cccgaaaaaa ccgtgaacgg tgaaccgctg gtcagtgtcg taatattcaa aatctgccat
     3001 ccacccgtta atttcttact gactggccga aggaaaccca ccttgtccag cggtagcgcc
     3061 tgaaagacgc tcagggtaaa cacgtgcgtc gcgtcaaatg tggacggccc tcgctccccg
     3121 tccatatcca gagggtcctg cggaaccgtt tgcaaaatca cgcccgtatt ccccgcaggc
     3181 ccgcccagaa tcgcgctcgt gttgtcaatc gacttcgaaa atgtgtagct ggcctgaaaa
     3241 gccatcccga tcttcgattg gttcatcgtc gcgccggtct gcagagcgtg atacgtcgaa
     3301 tgcgatccgt tggtcatcac cgcctctgga ccaaatccgc ccagcacatt tccattcgaa
     3361 tcaaatttcg taaacggcgc gaatccaggc gatgctcctg cgaacccgtt gggaaaccat
     3421 ccgttcggca agcgaacacc actcagtcct acgtaggctg ccgtcaactt catgcctgcg
     3481 agatcctgat caatccccgc cgtgtacgtt cccagatatc cattcttgaa gttccgagaa
     3541 ataatcccag gctggaacaa ctgaacttcg tttccaggtg ttagcgctgc gagatcattt
     3601 tggtatcgct gcagatcaat tacagtattt gccggtaccc gcgaactgtc cccgtttgga
     3661 aacagcagtt gtccgctcgt cgtgtatggg tcgggcagcg tcaccggaac cactgcatcg
     3721 gtaaacggca cgggggcacc cggcttcgcc gtcaccaccg gctgaaaagt cagcggaaat
     3781 ccaccggtca caaaattttg cagccacaaa ttcggcagca acgttgtaag ggctgctccc
     3841 gcgtgcagca tcatgtgctt ggttgcagca aaatccacgg aaacgcgcgg gccccaaccg
     3901 ttcttgtcca gcggatacac tggctgcggg ttgtacacaa aaatctgcga agcgcccggc
     3961 gtcaagaatg acgtctcgtg tccgttcgaa tccaggggta tcgcaatcga agtccgcttc
     4021 ttcgactcct tgattcggct gttcagttcg aatcgcaatc cgtagttgat ctttagccgc
     4081 gcattcactt tccaggcgtc catgaaataa aagtcgtaag cctcccgccg caccgaagcc
     4141 tcatcgaatt tgtcgcccgc cggcgtaaca ttcgccgctg ccgtgatcgt gtatgaaaac
     4201 ggcgtcgccg tcagaaaccc ggtcaacgcg tccggcaagg gctgcccggg caaaatgtcg
     4261 tgctgcccgc tcgctgaggt gatcaacacc ggcgcatacg ccgtccctcc tccaaacgca
     4321 tacagcccat tcggatttgt cccaaaaatc gtcgcatcct tattcaaccg aatctccact
     4381 ccccatttga gcgcatgcgt gccgcgcgta aacgtcatgt cttctttcgc ctgatacaaa
     4441 ttcccgaacg aaccaaagat cgatccgtcc gggttgttgt accccgcgta caacccatcg
     4501 ttgaaagtaa tcgccggctg cgtgtgattc tcggccggaa agaacggcgt gctgcgtatg
     4561 tacccgaatt gcgtcgtcga actcagccgc ggcgtgatcg ctttcgtgaa tcgcaccgtc
     4621 gcacttctct ggtgatcaaa gaatttcacc ccgaatgtgg gattgatcgc cgtctggtcc
     4681 gggttgttga tcggcccgtc tacctggtta aggctgaacc gccccatcaa cgcagctgtc
     4741 tccgaaaagt ggtggtcaat tcgaatcgaa aattggtccg tgttggtccg cactttcgaa
     4801 gaagtggcgt acgtccgctc gccgaacgca ccttgcggct cgttcggcat cgggtatccc
     4861 gccagaatcg cctttaccgc cggactcacc ggcacggtca gcgtttctcc aggaaacgcg
     4921 gtcgtatcca acccggcgcg ctcggtcgca gtcggtaccg ccagcacctg cgtcgttccc
     4981 agcacctgtc gaaatccctg atattctcca aagtagtacg tcctgccgcg cccgttgtac
     5041 aacttcggca acaccaccgg cccaccgttc gtcacgccaa actcattccg cgcaaatggc
     5101 ggtattcgcc gcgggtccag cgcgctcgcg tgatcaaaaa aattccgcgc atcaaacgcc
     5161 gcattccgca caaactcaaa cgcgctccca tgcacctgct cgccgccgct tttgctgatc
     5221 acgttcgtga atcccgcggc tccgtggccg atgtccgccg tcagcactcc tgaattcgac
     5281 tgcacttcct gaatcgcgtc cacgttgaag ttggcaaacg tcgctccgcc cagttccgga
     5341 tcggtagtgt ccgctccgtc gagcgcaaag accgtggccg aaccgcgctg cccgttcacc
     5401 gaaaactgct gcgtgaaatt tgcggcgccg ttcgtgtccg tcatcgtccc cgccgccagc
     5461 agcagcaact tgctgaaatc cctttcattc agcggcaaac tcgacacttc ctcgctcgaa
     5521 agcctctccc ctccgctccc ttgaatactc ttcccagttc tattctcttc ggtggccttt
     5581 tccgattctc cgggtgccgc acggttggtt tttaagggtg ggattcttcc aatttccaca
     5641 gacgccgtcc gtccatctgg ccccagctgc aacgccgtct ctaatgttgc tccagcttta
     5701 acatccaacg cttccgccgc cttccatatc ctgctcatca ctcgaacaac aacgtcatag
     5761 tctcctggct gaacgccggc aatcgtgaat tctccattcg caccggaagt tgtttggtat
     5821 tcgaccggcg cggcgccgct tgcgcgaagt tcaattgccg ccccggccac cgctttgccc
     5881 gcagcatcgc gtaccacgcc attcaattgt gccgtcgttt gcgcctcaac caagaaggga
     5941 agtagaaaca atacagaaga aaaaacagcc cggaacagca caccaggaag acccatgcgc
     6001 gcgattatac tcgctgcgtg cacgctctgc ggcaggcgat tccgcgagcg cccttcttca
     6061 cttcctgcag cagccaatcg ctgccgtccg aatggtcaaa catgtcaact agcgatagtg
     6121 gtgacgcggc ggtgccggtt tgcgacccac aggctcacat ggtcagaacg acgcgaaact
     6181 gcgcgttgcc gcttaacatg cgtgcgtaag cttctgccgc cttttccagc ggataagtct
     6241 cgatcatggc acgcacgccg gagagttcgg cgaagcgcag cgtgtcttca gagtccgctg
     6301 ccgtccctga ggcccagcct tgaatgctgc gacttccgga aatcagctgt atcggtgtta
     6361 cttcaatggg atcaaacgca gcgcctataa ccatgagttt gccgttcggg ccaaggccgt
     6421 cgatcaattc ggacatggcc ttggaactgg gcgccgtcgc cagaatgacc tgcgcaccgc
     6481 caagtttttg cagttcctcc gccgcgtttg tcgctttgct gtcaatgtac acgttcgcgc
     6541 ccagcttctt tgcaagcacg ccgttctcgg cgccgcgccc gatcgccgcc actctgtaac
     6601 caaacttgtt cgcaaattga actcccaggt gtcctaggcc gccgatccct tgcacggcaa
     6661 ccagatcgcc cggtgtagca ccgctgtggc gtagcgcatt gtaggtggtg attcctgcgc
     6721 acagcagcgg cgctgcttcc acgtcgctga gagtgtccgg tatcgcggcg agcgcctcga
     6781 cgggagcgac catatattgc tggtaaccgc cgtcatagct gattcctgca attttcagat
     6841 tcttgcaatt acgaaaatct ccgcgccggc aggagagaca cgttccatct tgcccgccgt
     6901 gccaaccgac acccacgcgc tgcccctgct tccattcggt tacgctcgca ccaacctcat
     6961 cgataacgcc cgcgacttcg tgtcccggca cgcgcgggta ctgaatcccc ggccaagcgc
     7021 cttctttggt gagcacgtcg ctgtggcaca ccccgcaggc ctgcaccttg atgcgtacct
     7081 gtcctgctcc cggcttgggt atctcgcgtt caacaatttc gaaatcgcct cccggtttgg
     7141 aaatctgcgc tgccttcatt ggcgatattg cgactttgga agttgccatg ttccctccta
     7201 cgaatctttg ctcgtcgctc tcgcagtcgt tgcacgcctg ccgttgttgt gcggttcggc
     7261 ggaagaggat gc
//
LOCUS       DASTPM010000676         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_485775, whole genome shotgun sequence.
ACCESSION   DASTPM010000676 DASTPM010000000
VERSION     DASTPM010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485775"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VFR42_11925"
     CDS             complement(<1..247)
                     /locus_tag="VFR42_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369915.1"
                     /translation="MFRAVALGEREGIHRPFVEAAKTWLARFSRENNFAVDYIEDTAK
                     IDDKFLSKYALFIQLDYPPYRWTPTAQAAFIKYIEHGK"
     gene            569..889
                     /locus_tag="VFR42_11930"
     CDS             569..889
                     /locus_tag="VFR42_11930"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU0369916.1"
                     /translation="MPARKLTDDEINKLLSRMSGWRTVNGKLHKTFECKDFNAAFGNM
                     TRVALVAEAMNHHPEWANVWNRVTFDLVTHSVKGISDLDFVLAEKIEEIFATQTASRA
                     TSGT"
     gene            986..1960
                     /locus_tag="VFR42_11935"
     CDS             986..1960
                     /locus_tag="VFR42_11935"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU0369917.1"
                     /translation="MIGSHQPANNSRALVITILRWVAAFAVLAILFHFLPVAPLRAAL
                     ARVPPIRFAIILVFYLCAHAIGIFKWRMVVNTAGSQLDVSTSAQCYLGGLFGTLFLPS
                     IIGGDVVRLAVGLRRSPRPAAVLAGNVVDRFLDVAAQATLVLLGLILLPGSLPPQFAA
                     LARKLLLAGTTVAAVIVILLMLLLKPLLAGRSRRFRRRQVRFRHAFRSVRRQPQVVVF
                     GWSLGVLIQGTFILLNALLAISCGLTLPLRVWLFAWPLAKLAALLPLTQGGMGIREAV
                     LVALLLPFGAASSLTLASGLVWEGIIICGGLISGLVAFLLRRNVRELS"
BASE COUNT          440 a          618 c          669 g          518 t
ORIGIN      
        1 ctttgccgtg ctcaatgtac ttgatgaaag cggcttgtgc ggtgggcgtc caacggtagg
       61 gcgggtaatc gagttggatg aaaagcgcgt atttcgaaag gaatttgtca tcgatttttg
      121 ccgtgtcctc gatatagtcc acggcaaaat tgttttcgcg ggagaacctc gcgagccaag
      181 tcttggcggc ttccacaaac ggcctgtgga tgccctcgcg ttcccccagc gcaacggctc
      241 gaaacattgg cgttctgtct cgggcagcga acgtggagcc cgggatgcag aagagcgcgg
      301 cgagcaagcc cgcgcgcagt aacgtgatcc gaactaggtg gctccgttga gacattgtgg
      361 tgaatcatcg acgttcgata aattgcgcac cgatcgaacg agaaatgttg taccacaaga
      421 gacatcgcgc gtgggtccgg ccagaagtag cagaaggtat gccagggggc aaagttctat
      481 ggcgaaggcg cgcctcccgc ttcataatat ctccgaggcc tgagcgatct tccatcccag
      541 acacagggcc tgatccacgg aggcacacat gccggcacgc aaacttaccg acgacgaaat
      601 caacaagctg ctgagccgaa tgtccggctg gcgcactgtg aacggaaagt tgcacaaaac
      661 ttttgaatgt aaggatttca acgcggcgtt cggcaacatg acccgcgtgg cgctggtcgc
      721 cgaagcgatg aatcatcatc cggaatgggc caacgtgtgg aaccgggtga cgtttgacct
      781 ggtgacgcac agcgtgaagg gcatcagcga cctggatttt gtgctggcgg aaaaaatcga
      841 agagattttc gcgacgcaaa cggcgagccg cgcgacgtca ggaacgtgac ccgacaattc
      901 ttttcccgcg aaggcgaaat ctgatggctg aatccgcata cgacgtccgc gatttcacca
      961 ccggcaaaag cacgacccgc acagcatgat cggctcccac cagccggcaa acaactcccg
     1021 cgcgttggtc attaccattc ttcgttgggt ggccgctttc gccgttctgg caattctctt
     1081 tcattttctt cccgtcgctc cactgcgagc cgcgctggcg cgggttccac cgattcgctt
     1141 tgccatcatt ctggttttct atttgtgcgc gcacgcgatt ggaattttca aatggcgcat
     1201 ggtggtgaac acggctggat cacagctaga tgtttccacc agcgcgcagt gttacctcgg
     1261 cggactgttt gggacgctgt tcctgccatc gatcattggc ggcgatgtgg tgaggctggc
     1321 ggtgggatta cgccgcagcc cccggcctgc cgcggtgctt gccggaaatg tggtggatcg
     1381 ttttctggat gtggctgcgc aagcaacgct ggtactgctt ggactgattc tacttccggg
     1441 ttcgctgccg ccacagttcg ccgcgcttgc gcgcaaactt ttgctggctg gcacaaccgt
     1501 cgcagcggtg atcgtgattt tgctgatgct cctattgaaa ccactcttgg cggggcgatc
     1561 gcgtcgcttt cgccgaaggc aggtgcggtt tcgtcacgcg ttccggtcgg tacggcgtca
     1621 accgcaggtg gtcgtttttg gctggtcgct cggggtgctg attcaaggaa cctttatttt
     1681 gttgaacgcg ctgctggcga tttcctgcgg actgacttta ccgctgcgcg tgtggctttt
     1741 tgcatggccg ctggcaaagc tggccgctct gcttccgcta acacaaggcg gcatggggat
     1801 tcgcgaagcg gtcctggttg cgctgctgct gccgtttggc gcggcgagtt cgctcacgct
     1861 ggcgtccgga ctggtttggg aaggcatcat tatttgcggc ggactgatct ccggactggt
     1921 cgcgtttctg ctgcggcgaa acgttagaga actctcatga cgcatggcaa cgctgcactg
     1981 aacttgctgg aaacaacacg aaacgcttag agagaaatta tctctccctt gcaacagaag
     2041 tggtgtcccc cggaggactg tcctctcaga gcctcctaaa cgacagtagc tcgtgactga
     2101 cattaatcat cgattttcga gcctttcggc atttgcgtat tttcgcaaag cagttgcgcc
     2161 acgagcacgc cgaccgatga cccgatttcg gaagccgctg cgtttctgca ggagctgaac
     2221 gccgactgcg cgctggtttt tcagc
//
LOCUS       DASTPM010000677         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_975733, whole genome shotgun sequence.
ACCESSION   DASTPM010000677 DASTPM010000000
VERSION     DASTPM010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975733"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFR42_11940"
     CDS             <1..773
                     /locus_tag="VFR42_11940"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369918.1"
                     /translation="YGVRRDRADVMGIAGIIFITLGRYLNVRLLAIPAVGIREGLIHE
                     IAKEAFSRKEPHRYTAAARQMLVGARSFSRRLERDPRHAEHVRELSLQLFDQLQPLHH
                     LPSDARIILEAGALLHDIGHTVSHKGHHKHGEYLTMNGDIAGLDGRERALAAAVVRYH
                     NRKAEPAEHHISYAALNTSDRRLARRLASLVRIAEGLDHSHRQRIGSIRAAFQRGAVG
                     LHLQARGDASEDVLDASRSAELFEKEFHTKLYFRPLND"
     gene            819..1328
                     /gene="sixA"
                     /locus_tag="VFR42_11945"
     CDS             819..1328
                     /gene="sixA"
                     /locus_tag="VFR42_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEU0369919.1"
                     /translation="MQLYIVRHGTAIDREDPKSPSDPERYLTPEGLRKTREVARGFAS
                     LKISPDVFLSSPYVRAMQTAEIFAEETKFSKSKIKQTDLLLPGADPAAFFRELQRTSR
                     SEESAICFGHAPHLDELIAFALGSKKDLTQLKKAGAACLELTRISPPSGVLAWLSTPK
                     ILRKRTKGD"
     gene            complement(1330..2841)
                     /locus_tag="VFR42_11950"
     CDS             complement(1330..2841)
                     /locus_tag="VFR42_11950"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU0369920.1"
                     /translation="MAFAVAIPSRALLEHRDLAFWMKRSLEELAELRANPSADAVHDL
                     RVSLRRCRSLASAVEEIDPHADWQEMRDGARKLFRSMGELRDAQVAQEWLNKLRPEVD
                     PLKVMLLGLLKQTQESALAKALHLAEKFDEKRWAEMAKALHSRLRKIPADREAARCLA
                     LERLQEAHELHRRALRTERPAPWHDLRIALKRFRYTLELLAPTLHSKCIGSLKRVQDV
                     LGGIHDLDMLGELAAQTKDAVTPELRQDWQARVENVRQERMQTYRQLALGGVSIWQDW
                     LSSFPKLDWPEYAAARIAATRKAMDPKPRRSLAVSRIAAKLWTELHRCRAGPIFADQN
                     EKRVVVAAARLSGIAYESKKKSRLKSVRTFLLKSPVPPAWTFGEWERAAWCIRFQRGP
                     EPITGNKRFARLSGEQQAAIRNLAGVLRLAVAAQRSGAASGAAIKMDRLSQGLLLHVD
                     GVEETPDVAARFTEAKHLLERSIGKTILIQPTKAPDLNTANEVVRLTPVVVLR"
BASE COUNT          612 a          908 c          928 g          620 t
ORIGIN      
        1 cctacggagt gcgacgcgac cgggccgatg tgatgggcat cgcggggatt attttcatta
       61 cgctggggcg gtacttgaat gtgcgcctgc tggcgattcc tgcagtgggc atccgcgaag
      121 ggctgattca tgaaattgcc aaggaagcgt tttcgcggaa ggagccgcat cgctacaccg
      181 cggctgcgcg gcaaatgctg gtgggagcgc gcagcttttc gcggcggctg gaacgcgacc
      241 cacggcacgc cgagcacgtc cgcgagctga gcttgcaact gttcgaccaa ctgcagcctt
      301 tgcatcacct gccgtcggac gcgaggatta ttttagaggc cggtgcttta ctgcacgaca
      361 tcgggcacac ggtaagccac aagggccatc acaagcacgg cgagtatctg acgatgaacg
      421 gcgacattgc aggattggac ggacgagaac gcgcgttagc cgccgcggtg gtgcgttatc
      481 acaaccgcaa agccgagccg gcggagcatc acatttccta cgcggcgctg aataccagcg
      541 accgtcgcct ggcgcgtcgg ttggcttcgc tggtgcgcat tgcggaaggg ttggaccact
      601 cgcatcggca gcgcatcggc tcaattcgcg cggcgttcca gcgcggcgcc gtaggcttgc
      661 acttgcaagc gcgcggcgat gccagcgaag acgtcctcga cgccagccgc agcgctgagc
      721 ttttcgaaaa ggaattccac accaagctct acttccggcc tcttaacgac taaactatgc
      781 gaagggccac gcgtgaacac gaggaggaag catccgcaat gcagctgtac atcgtaaggc
      841 acggaactgc gattgaccgg gaggatccaa agagtccttc cgatccggag cgttacctga
      901 cgcccgaggg gctccgaaag acgcgcgaag tggcgcgcgg atttgcttcg ctgaagattt
      961 ctcccgatgt tttcttatcg agtccctatg tgcgggcgat gcaaaccgca gagatatttg
     1021 ctgaggagac aaaattttcg aagtccaaga ttaaacaaac cgaccttctg ctgcccggag
     1081 ccgatcccgc ggcgtttttc cgggagttgc agcggacgtc gcggtcggag gagtccgcaa
     1141 tttgtttcgg tcatgcgccg cacctggacg agctgattgc ttttgcgctg ggttcgaaaa
     1201 aggatctgac gcagttgaag aaggcgggag cggcctgcct ggaattaacg cgcatcagcc
     1261 caccttccgg agtgctggcc tggctgagta ctccgaagat tttgcgcaag cgaacgaagg
     1321 gagattgatt tagcgaagga cgacgacggg cgtcagccgt acaacctcat ttgccgtgtt
     1381 gaggtccggc gctttggtcg gctggatcaa aatggttttg ccgatgctgc gttccagtag
     1441 atgcttggct tccgtaaaac gtgccgcgac gtccggagtc tcctctacgc cgtcgacgtg
     1501 aagtaagagc ccttgcgaca acctgtccat ttttatcgca gcgccagaag ccgctccgct
     1561 gcgctgcgcc gcgacggcca gtcgcagaac tcctgccagg tttcgaatcg cggcctgctg
     1621 ttcccctgac agcctggcga atctcttgtt gccggtaata ggctcgggcc cgcgctgaaa
     1681 gcgaatgcac caggcagcac gttcccattc gccgaaagtc caagctggcg ggacgggcga
     1741 cttcagcaga aaagtgcgca ccgacttcag tcgcgatttc tttttcgact cgtacgcgat
     1801 cccgctcagc cgcgccgccg ccaccaccac gcgcttttcg ttttggtccg cgaaaatcgg
     1861 accggcgcgg caccggtgca gttcggtcca gagcttcgca gcaattcgcg aaacagccag
     1921 gctccgacgt ggcttcggat ccatggcttt gcgtgtcgcg gcaattctgg cggcggcata
     1981 ttccggccaa tccagtttcg gaaaactgct gagccagtct tgccagatgc taactccgcc
     2041 caaagccaac tggcggtagg tttgcatcct ctcctgccga acattctcca cgcgcgcctg
     2101 ccagtcctgc cgcaactccg gagtgaccgc gtctttggtt tgcgccgcca attcccccag
     2161 catatccagg tcgtggatgc cgccgagaac gtcctgcacg cgcttaaggg agccgatgca
     2221 tttgctgtgc agcgttggag ccagcaattc cagcgtgtag cggaaacgtt tcagcgcaat
     2281 tcgcaaatcg tgccagggcg ctgggcgttc cgtgcgcaac gcacgccggt gcaattcgtg
     2341 cgcctcttgc agccgttcaa gcgccaagca cctcgcagct tctcgatctg ctggaatctt
     2401 tcttaaacgc gaatgcagag ctttggccat ttcggcccag cgcttttcat caaacttctc
     2461 ggccagatgc aaggctttgg ccaacgcgga ttcctgtgtt tgcttcaaca acccgagcag
     2521 catgacctta aggggatcga cctcgggtcg aagcttgttg agccattctt gcgcgacctg
     2581 ggcgtcgcgc aattcaccca tcgatcgaaa cagcttgcgg gcgccgtcgc gcatctcctg
     2641 ccagtctgca tgggggtcga tttcttccac agcggaggcc agcgaccggc aacggcgcag
     2701 agaaacgcgt aggtcgtgaa cggcgtcagc cgaggggttg gcgcggagtt ccgccagttc
     2761 ttccagcgac cgcttcatcc agaacgccag gtcgcggtgc tcaagcagag cacgagaggg
     2821 aatggcaacg gcgaaagcca tgtttggtaa agccgctccg gcagaacttg gtagttcggc
     2881 ccctgtggag cggaacagta gggtaccgct ccgctcccat tgcgtccagc aggtaccccg
     2941 tgtatgccgt tgagtcctgt ggatgaatca ccgagttcac gttatttttg aagaatacgg
     3001 ccggagtaac ggaatggcag acgcggcgga atcaaaatcc gctgaccgca aggtcgtgaa
     3061 ggttcgac
//
LOCUS       DASTPM010000678         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_197469, whole genome shotgun sequence.
ACCESSION   DASTPM010000678 DASTPM010000000
VERSION     DASTPM010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197469"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /gene="murD"
                     /locus_tag="VFR42_11955"
     CDS             <1..1471
                     /gene="murD"
                     /locus_tag="VFR42_11955"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU0369921.1"
                     /translation="CGAGVRAVCADDFEIEVAMPGPVELKNKRVLVVGLARTGIATAL
                     FCAARGASVTATDSRGEAEIGAEVATKLRAANVKLELGGHREETFLQRELIVPSPGVP
                     ADAPLLQAARAKGVPIWSEIELAYRFRHGKLIGITGSNGKTTTTSLVEHILRKASLPT
                     ILAGNIGTPLISCVERMTHDTVTVVELSSFQLELIQEFRTDISVFLNLTPDHLDRHHT
                     LELYSKAKARIFENQREEDFAILNADDPGTTPLAPTRPQVYWFSRKQRTAQGAFLRGA
                     EIVFRADGREEVVLQREEVPLAGAHNLENVLAAVAAARLAGASGKAVRDRVMSFAGVE
                     HRLEFVAEINGVRYFNDSKATNVDATLKALDAFPGRILVILGGKDKGSDYTTLRMPLR
                     ERAILALLIGAAADKIDSEIAGSVAIERAGTLEHAVEIAFEAARAGDVVLLAPACASF
                     DQFENYEHRGRVFKALVQELKQRANEMPSTNRGATSARR"
     gene            1477..>2284
                     /locus_tag="VFR42_11960"
     CDS             1477..>2284
                     /locus_tag="VFR42_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU0369922.1"
                     /translation="MPRRSENDRWLFGVTLALCLLGAVMIYSASAVTADQQYGRSYIF
                     LLRQAAWLLIGLLGMFALMRTDYRRLREPAVVYPTIFLVLLMLVGAFFLDKSHATHRW
                     IRFGPVGIQPSELAKLAVVLYLAWFLDLKRRGASTMEFRKEDFLHTILPAAGPILVCV
                     VLILLQPDLGTSVDIVLITAAILFVAGLSWKWLVTGGVAALPILYLLITHVSYRQARL
                     MAFLDPGSDPQGAGFQLLQSLIAVGSGGFTGVGLMESKQKLFYLPEAHTDF"
BASE COUNT          413 a          598 c          793 g          480 t
ORIGIN      
        1 ttgtggcgct ggtgttcgcg ctgtttgcgc tgacgacttt gaaattgagg tagctatgcc
       61 gggcccggtg gaattgaaaa ataagcgcgt gctggtggtg gggttggcgc ggacgggaat
      121 tgcgacggcg ctattttgcg cggcgcgcgg ggccagcgtc acggcgacag actcgcgcgg
      181 ggaagcggaa atcggcgccg aggttgcgac gaagttgcgc gccgcgaatg tgaagctgga
      241 gcttggcggg catcgcgaag agacatttct gcagcgggag ctgatcgttc cgagtccggg
      301 cgtgcccgcg gacgcgccgc tgctgcaagc ggctcgcgcg aagggcgttc cgatctggag
      361 cgagattgaa ctggcgtacc ggttccggca cggcaagctg atcggaatca ccggatcgaa
      421 cgggaagacc acgacgactt cgctggtcga acacatcttg cggaaggcgt cgctgcccac
      481 gattctggcc gggaatatcg ggacgccgct gatttcttgc gtggagcgga tgacccacga
      541 cacggtgacg gtggtggagt tgagcagctt tcagctggag ctgattcagg aatttcgcac
      601 ggacatcagc gtgtttctga atcttacgcc agaccatctg gaccgccatc acaccctgga
      661 gttgtacagc aaggccaagg cgcggatttt cgagaatcag cgcgaagaag atttcgcgat
      721 tttaaatgcg gatgatccgg gcaccacgcc gctggcgccg acgcggccgc aagtgtactg
      781 gttcagccgc aagcaacgca cagcgcaggg agcattcttg cgcggcgccg aaatcgtgtt
      841 tcgcgctgac gggcgggaag aggtggtgct gcagcgggaa gaagtgccgc tggcgggcgc
      901 gcacaatctg gaaaacgtgc tagcggcggt ggccgcggcg cggctggcgg gtgcgagcgg
      961 caaagcggtg cgagatcgag tgatgtcgtt cgcgggggta gagcaccggc tggagtttgt
     1021 tgcggagatc aacggtgtgc gttacttcaa tgattccaag gccacgaatg tcgacgcgac
     1081 attaaaggcg ctggatgcgt ttccggggcg catcctggtg attctgggcg gcaaagacaa
     1141 gggcagcgat tacacaacgc tgcgcatgcc gctgcgtgag cgggcgattc tggcgctatt
     1201 gattggagcg gcggcggaca agatcgacag cgagattgcg ggcagtgtag cgatcgaacg
     1261 cgccggcacg ctcgagcacg cagtcgagat tgcttttgag gcggcgcgcg ccggcgacgt
     1321 cgttctgctc gctccggcct gcgcaagttt cgatcagttt gaaaattacg agcatcgcgg
     1381 gcgggtattc aaggcgctgg tgcaggaatt gaagcagcgc gccaacgaaa tgcccagcac
     1441 gaatcgcggc gcaacttccg cgcggaggtg agctgagtgc ccagacgttc ggaaaatgac
     1501 cggtggcttt tcggtgtgac gctggcgctg tgcctgctcg gcgcggttat gatttacagc
     1561 gcgtcggcgg tgaccgccga ccagcaatac gggcgctcgt acattttttt gttacggcag
     1621 gcggcctggc tgctaatcgg gctgctggga atgtttgcac tgatgcgcac cgattaccgg
     1681 cgattgcgcg aaccggcggt ggtgtacccg acgatttttc tggtcttact gatgctggtg
     1741 ggcgcgttct tcctggataa gtcgcatgcc acccatcgct ggattcgttt tggcccggtg
     1801 ggaattcagc cttcggagct ggccaaactg gcggtggttc tatacctggc atggtttctc
     1861 gatttgaagc ggcgcggcgc gtcgaccatg gaatttcgca aggaagattt tctgcacacg
     1921 attttgcccg cagcgggacc aattctggtg tgcgttgtgc tgatactcct gcagccggac
     1981 ctgggaacgt cggtcgatat cgtgctgatc accgctgcga ttctgttcgt ggccggcctt
     2041 tcgtggaaat ggctggtcac cggcggtgtc gcggcgctgc ccattttgta cctgctgatt
     2101 acccacgttt cgtaccgaca ggcgcggctg atggcgtttc tcgaccccgg ttcggacccg
     2161 caaggcgcgg gctttcagtt gctgcaatcg ctgatcgcgg tcggctccgg cggttttacc
     2221 ggtgtgggat tgatggagag caagcagaaa cttttttatt tgccggaagc gcacaccgat
     2281 ttta
//
LOCUS       DASTPM010000679         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_889436, whole genome shotgun sequence.
ACCESSION   DASTPM010000679 DASTPM010000000
VERSION     DASTPM010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889436"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VFR42_11965"
     CDS             <1..313
                     /locus_tag="VFR42_11965"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU0369923.1"
                     /translation="EYSFDRSDAKAIRSEFELHEKQAKELLEGWKTAAGKERRRYPLL
                     ATYDHCLKCSHLFNLLDARGVISTTERAALMARVRSVACRTAATYLEQLTPAAAAEVR
                     A"
     gene            310..>2093
                     /gene="glyS"
                     /locus_tag="VFR42_11970"
     CDS             310..>2093
                     /gene="glyS"
                     /locus_tag="VFR42_11970"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU0369924.1"
                     /translation="MSRAKLKPAKKRPAVELPPPGRKLELLFEVGCEEIPAGMLARAT
                     EDLKAGLEKQLAVESLAEGVLVEAFSTARRLVLWARGLPLRQADVVNEVTGPPKSVAY
                     DPVGSPTRAAVSFAEKQGIALHEVYFVQTPKGEYLAAKQTKRGRATEFILADALPRIL
                     HDLYWPKTMTWTGLSGARFIRPIRWIVGLLDGQPLKFSYGGIAASDVTRGHRFLGSSA
                     IRVKTFADYEKSLRTNGVLVRPSERQEKIERELAAAAKKGSYRIHEDAELLKLVTYLN
                     EFPSVIEGNFDPAFLNLPDEILVTVMRGHQKYFAVEKRNGELAPHFLAVINLAKDSKG
                     LVRAGHERVLKARFADAQFFWESDQKCRLADYLPKLERVTYESRLGSYRDKVERIRSL
                     ARWLCEQWYNLGLAQAHVSDADRAAELAKCDLATEMVREFPELQGVVGGLYSKAQGEP
                     DAVSDAVYDHYRPVGLDDPIPRNLIGCAVALADKLDSIVGCFAVGVVPTGSSDPFALR
                     RAGLGMVKIILEKKLPLSLVDVIAAAAKALHVNPPKRQVTPEQEKQVLEFLLERAKFV
                     FREREGLGYDEVNAVFRASAEDLVDARRR"
BASE COUNT          432 a          597 c          656 g          408 t
ORIGIN      
        1 ggaatacagc tttgaccgca gcgacgcgaa agcgatccgg tcagagttcg agctacacga
       61 aaaacaagct aaagaattac ttgaaggttg gaaaacggca gcggggaaag agcgtcgccg
      121 ctaccctttg ttggcgacgt acgaccactg cctgaaatgt tcgcacctgt ttaatttgct
      181 ggatgcccgc ggtgtgattt cgacaacaga gcgagccgcg ttgatggctc gcgtgcgttc
      241 ggttgcgtgc aggaccgccg cgacgtactt ggagcagcta acgcctgccg cagcggccga
      301 ggtgcgggca tgagccgagc caagctgaag ccggcaaaaa agaggcctgc ggtagagttg
      361 ccccctcccg gcagaaaact ggagctgttg tttgaagtgg gctgcgaaga aattcctgcg
      421 ggcatgctgg cgcgtgccac ggaagacttg aaggccgggc tggaaaagca gttggcggtc
      481 gagagcctgg ccgaaggcgt actggtggag gcgtttagca cggcgaggcg cctggttctt
      541 tgggcgcgtg gactgccgct ccggcaagcg gacgtggtga acgaagtgac tggcccgccg
      601 aagtcggtgg cgtacgatcc ggtcggctcg ccgacacgcg ctgccgtaag cttcgccgag
      661 aagcagggca tcgctctgca cgaagtctat tttgtgcaga ctcccaaggg cgaatacctc
      721 gccgcgaaac aaaccaagcg cggcagggca accgagttca ttctggcgga tgcattgccg
      781 cgcattctgc acgatctcta ttggcccaag accatgactt ggactggtct ttccggtgcg
      841 cggttcatcc ggccaattcg ctggatcgtg ggcctgcttg acggccaacc gctgaagttt
      901 tcttatggcg gaatcgccgc ctccgacgtc acgcgaggac accgtttcct gggctcttcg
      961 gcgattcgcg tgaaaacctt tgcggactat gagaagagct tgcgcaccaa tggagtgttg
     1021 gttcgcccgt ccgagcggca ggagaagatc gaacgcgaac tcgcggccgc cgcaaagaag
     1081 ggaagctatc gaatccacga ggacgcggaa ctgctgaagc tggtgaccta cctcaacgag
     1141 ttcccctcgg tgatcgaagg caacttcgac ccggcgtttc tgaatctgcc agacgaaatt
     1201 ctagtcacgg taatgcgtgg gcaccagaaa tactttgccg tcgagaaacg caacggggaa
     1261 ctggccccgc attttctggc ggtaatcaac ctggcaaagg attccaaggg attggtacgc
     1321 gcaggtcatg aacgggtgct taaggctcgc ttcgctgatg cgcagttctt ttgggagtcc
     1381 gaccagaaat gtcggctcgc cgactatctg cctaagctcg agcgagtcac ctacgaatcc
     1441 cgcctcggca gttaccgcga caaagttgag cgcatacgct cgctggcacg ctggctttgc
     1501 gagcaatggt acaacctcgg cctggcgcag gcccatgtat cagacgccga ccgcgccgcg
     1561 gaacttgcca agtgcgacct cgccacggaa atggtgcgcg agtttcccga gctgcaaggc
     1621 gtcgtcggcg gtctctactc caaggcacag ggcgagccag atgctgtctc ggacgccgtt
     1681 tacgaccact accgtccggt tggtttggac gatcccattc cgcgcaacct gatcggctgc
     1741 gccgtggcat tggccgacaa actcgattcc atcgttggat gttttgccgt tggtgtggta
     1801 ccgactggtt cgagcgatcc ttttgccctc cgccgcgccg gcctcggcat ggtcaagatc
     1861 atcctggaaa aaaagctgcc gttatcgctc gtggatgtca tcgcggcagc tgccaaggct
     1921 ctgcacgtca atccgcccaa gcgccaggtc acgcccgagc aagagaaaca ggtgctggag
     1981 ttcctgctcg agcgcgccaa gttcgtcttc cgcgaaagag aaggcttggg ctatgacgaa
     2041 gtgaatgccg tgttccgcgc cagtgccgaa gatctagtgg atgcgcggcg acg
//
LOCUS       DASTPM010000680        12033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1379674, whole genome shotgun sequence.
ACCESSION   DASTPM010000680 DASTPM010000000
VERSION     DASTPM010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379674"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFR42_11975"
     CDS             complement(<1..692)
                     /locus_tag="VFR42_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU0369925.1"
                     /translation="MKNRTGDTKVGSEAFGIAALLRPHWRAISLAFVAVVGEIFADLL
                     EPWPLKMVIDYVLQSKKMPDWLAGMVSKVAGHDPIAVLNAAVIGVAVIAIIGAISTYA
                     EKYLTTSVGQWVTHDLRRTLYHHIHRLSLSQHDATRVGDLIGRVTSDIEAIQSFIVTA
                     LLGSVVSVLTLLGMVGVMFYLNWRFTLIALSVAPALFGVVFYYTRRIKKASREVRKKE
                     SELVSVVQEVLSS"
     gene            862..1620
                     /locus_tag="VFR42_11980"
     CDS             862..1620
                     /locus_tag="VFR42_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369926.1"
                     /translation="MFCPKCGAEYRPGYTSCADCHVFLVHERSGAHQLARLQRTEADR
                     DVEGSRANADTNYDYPGADRVPAEPGDPNEDPFCSFWKGTDARVCAELCTVLDEAGIP
                     HKTIHRQDHLFNFANQSPYELGVPASLYEQAELAVKEAFGTDEETGEDAVPLLPAPDG
                     EPADFEKGLRDAIADANEWLGPRYPEDATVEVWSGEDAEKDTKEMIEMSLRENDLLCR
                     SEEENGRIKLLVLPEDEERARQIVREIVEGAPLG"
     gene            complement(1648..2256)
                     /locus_tag="VFR42_11985"
     CDS             complement(1648..2256)
                     /locus_tag="VFR42_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369927.1"
                     /translation="MYCEHCGLQIFPKHPICTRCGESPTYQLFQLASLGTLFLVVLCN
                     GLVAILLLPRFSQAHYAGFYFRSWLWLTDKAAIYGWAPLAAGILAWDYFVRRKSRQKI
                     KSWLTRKLLSFVLVASVTPVLPWWIPAGQPPQNFMSLIHTYPGLPSGLAWLAISLVII
                     ALCSNAESRLALLGTGRVLSAISLASLFVLLTLTVLGWTLAS"
     gene            2531..4177
                     /locus_tag="VFR42_11990"
     CDS             2531..4177
                     /locus_tag="VFR42_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0369928.1"
                     /translation="MAQHYDVIVIGGGHNGLTNAAYLAKAGKKVLVLERRHVLGGAAV
                     TEEIIPGFLFSECSYVVSLLRPEIIRDLDLPRHGLEILPLDGTFTPMPNGDHLWRMND
                     HGRTVREIRRHSRVDAEAYEEFSKMMTPMCRFVKPILSMIPPDPTTLRPKDLMQLNFL
                     MQRFRSLSSDERYTLIQLMTMSAADFLDQWFETDVLKATMSASGIIGTFLGIRSPGTA
                     YVLLHHYMGEIDGAFRSWGFSRGGTGAISNAIAAAAREAGVEIRTKAGVAKIIVKGGR
                     AAGVALTSGEEISANVVSSSVDPHLTFEKFLEPNELPGDFLEEVKRYKFRGSSGKVNL
                     ALDGLPEFKCMPGDGAHLRGALSISPSVEYMERGYDDAKYGNFSRRPYVDMVIPSLTD
                     PSVAPPGKHVLSCFVQYAPYKLAKGTWDDQREAFGDNVIDTLSEYVPNLKKIIIGRQV
                     LTPLDLEREFGLTQGNIFQGELSLEQLFFLRPVPGWAYYKTPIESLYMCGSATHPGGG
                     IMAAPGRIASQVILKEWKGGSSSKNNPSRSTAPAAARAGN"
     gene            4180..5778
                     /locus_tag="VFR42_11995"
     CDS             4180..5778
                     /locus_tag="VFR42_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0369929.1"
                     /translation="MPLGQKVVIVGGGHNGLIAAFYLAKGGFKPVVLERREMVGGGAV
                     TEEFHPGFRASTLAHTLGPLRADVARDLGVEKFDCRIFEPDPRVFSPSLDGRGLLFYS
                     DAAKTAGGIARISSKDAEKYPQFSEALEEVAGVFAQLSSITPPAIDKPTPEDLWNLFK
                     TGRGVRGLGKKRIFDLLRWGPMAVADFVAEFFETELIRATIAARGIFGTALGPWSAGS
                     TAVLLMRAAADANPVGSAAFARGGLGNFSRALAEAAKQAGAEIRTDAEVQQIRTKDGA
                     VVGVVLRDGEEIATEAVVSGVDPKRTFFGLLDPTQLDPRFAQRMKNVRMKGTVAKVNL
                     ALGDLPSFPSLADAIGQDGYLRALSGRIHIGPEIDYLERAFDVSKYGEFPSAPWLDVT
                     LPTILDPSLAPDAKHILSAYVQFAPFTLRDGSWDSRRKELGEVVIKTLAPYAPNLPGL
                     VEGMQVITPADLEKSYGFTGGHIFHGELALDQLFTMRPALDWARYKTPVRGLFMCSNG
                     THPGNGLTGASGANAAREIIHELR"
     gene            5775..7127
                     /locus_tag="VFR42_12000"
     CDS             5775..7127
                     /locus_tag="VFR42_12000"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0369930.1"
                     /translation="MSAIKREKLHQLKQREEARFIAEHPKSAELYARAKHSLLGGVPM
                     NWMNKWAGAFPVFVAKAKGAHFTDVDGHEYVDLCLGDTGAMTGHSPDVVADAILKRVR
                     EGITLMLPTEDSLCVGQELKHRFALPYWQFALTATDANRFAIRIAREITQRPKILVYH
                     YCYHGTVDESFASLHNGVVGPRRGNIGPPVNPAETTRVVEFNDLNALEDALADHDVAV
                     LLAEPAMTNVGIILPDDGYWKAARALTREYGTLFIADETHTICAGPGGCTKEWKLDPD
                     FVVFGKPIGSGIPGSTYGVTEEVAKRISARIELEDCDVGGIGGTLAGNALSLAAMRAT
                     LENVLTAEAYKTMIPLAQRFNDGVAAEIKKNALPWNVIQLGARAEYHFTPKPPRSGGE
                     SAAAADFELERLLHLYALNRGVLLTPFHNMALMCPATTAADVDLHTAIFAEAAAELVA
                     "
     gene            7164..8738
                     /gene="pruA"
                     /locus_tag="VFR42_12005"
     CDS             7164..8738
                     /gene="pruA"
                     /locus_tag="VFR42_12005"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEU0369931.1"
                     /translation="MATAEVKLQKPEFANEPFVDFSKLENRAAMEAALKKVAAEFGRE
                     YPMYIGGQKATTTEKMHSTNPSHPSQVIGIVQKASAEQAKQAIESAHGYFDTWKKVPA
                     EQRAEILFRAAAIIRQRKFETSALICYEVGKSWPEADADTAETIDFLEYYGREMLRLA
                     GPHKVVPYQNERNFMVYIPLGAGAAIPPWNFPAAIATGMTAAALVAGNTVVLKPSEES
                     PVIAAKMVEILYQAGIPKEALQFITGEGEVAGDALVKHPKTRFIAFTGSKDVGLLISE
                     AAGKRQPGQLWIKRAVLEMGGKDATVVDEEADVDAAVEGVAQAAFGYQGQKCSACSRA
                     IVSEKVYDTFVQKLVDRAKKVTVGPSEDAANFMGPVVSEGAMNGILNYIEVGKKEGKV
                     VLGGGRAPGDGYFVQPTIIADVSPKARIAQEEIFGPVLAVIKAKDFDEALQIANDSEF
                     GLTGAVYSKNRAKLEKAEEAFHVGNLYLNRKCTGAMVGAHPFGGFNMSGTDSKTGGKD
                     YLLHFLQAKSIAEKPQ"
     gene            complement(8747..9679)
                     /locus_tag="VFR42_12010"
     CDS             complement(8747..9679)
                     /locus_tag="VFR42_12010"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0369932.1"
                     /translation="MKLQLRWLSLAVLVALFPATNNAQTLAEKLGYPKDAKLLIIHAD
                     DVAETHAVNAATFQAVESGAVNSASIMVPCPWFPEAADYARSHPEVDFGLHLTLTSER
                     VYYRWGPVAARNSVPSLVDSNGYFHHDWDQHPRIDPRDVETELRAQIERARAMGVRPS
                     HFDSHQYRLIENGKEIFQVFVRLGHEYGVPIFVARDWFSEHPYLEASLGPRDLILDHT
                     VTISPDVPAEKWADFYRDAVKNLQPGVTEFVIHVGLADEELRAATRERATWGAAWRQR
                     DFDFFMSKEFRSLLEQQRVKLITWRELATAAASR"
     gene            complement(9676..11058)
                     /locus_tag="VFR42_12015"
     CDS             complement(9676..11058)
                     /locus_tag="VFR42_12015"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0369933.1"
                     /translation="MSTTLDASSSAGTARPSRGRLLQVLGMWFGIAAAIGNTIAAGIV
                     AAQGYIAAQLPNVWLFLGVWILGGLYAIFGASSLAELGAAIPRSGGQYNYSRRALGEY
                     AGFVVGWSDWISTCGTNAVVALVIGNYTGVLFPALAGRDATIAVIILLGFAVLQWRGI
                     KWGSAAQLITAALKTGAFLVLVIACFVFGGSWHAAAVAASTPLPLPGGWLLATAFMLA
                     LQKVIYTVDGWDGVIYFGEEVKDPGRDVPRAIFGSVFSIIGIYLLLSAAVAYVLPMNE
                     IVGNGFALGTAAARIFGTYGDPVIRSIMLISLLSCINACLLFSTRTLYAMSSDGLFFR
                     AASRVNSGGTPVLAMVLSTLVGISFCIFQKFIRVIDMLAFFFVANYTLSFISTFRLRS
                     RAPDLPRPYRAWGYPWTTGVALAASLLFLAGALYTDRVNTPWAFGILVASYPIFRVLK
                     MVSPTRGSKA"
     gene            11191..>12033
                     /locus_tag="VFR42_12020"
     CDS             11191..>12033
                     /locus_tag="VFR42_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0369934.1"
                     /translation="METKRTSEPGPQLTTQLVTDVPTETVDVSVVIPCLNEANSLRVC
                     IEKAQKAFKEARLRGEVVVADNGSTDGSIEIAQTAGARVIPVAQRGYGSALKAGIRAA
                     RGAFIVMGDADDSYDFSEVPRFVEEWRKGSEVVMGNRFRGEIKPGAMPWHHKYIGNPG
                     LTAMLNVLFRSGIGDSHCGMRGFTRDVFERMDLRSSGMEFASEFVIKAAQIGAKTCEI
                     PITLWKDKRGRPPHLRSFRDGWRHLRFMLLYAPNWLFLLPGGSLMLLGLALVFWLLPA
                     PRTIG"
BASE COUNT         2645 a         3295 c         3857 g         2236 t
ORIGIN      
        1 gatgaaagaa cttcctgcac tacggaaacc agttcgctct ctttcttccg cacttcgcgc
       61 gacgctttct tgatccgccg cgtgtagtaa aaaaccactc caaacagcgc cggcgccacc
      121 gacagcgcaa tcagcgtgaa ccgccagttc agatagaaca tcacgccgac catgcccagc
      181 agcgtcagca cgctcaccac cgagcccagc agcgccgtca cgataaaact ttggattgcc
      241 tcgatatcgc tcgtcacgcg cccgatcaaa tcgcccactc gcgtcgcgtc gtgctgcgaa
      301 agcgacagcc ggtggatgtg gtgatagagc gtgcgacgca aatcgtgcgt cacccactgc
      361 cccacgctcg tggtcagata tttttccgca tacgtgctga tcgcgccgat gatcgcaatc
      421 accgccacgc caatcaccgc cgcgttcagg accgcgatcg ggtcatggcc cgcgaccttg
      481 ctgaccattc cggccagcca gtccggcatc ttcttcgact gcagcacata gtcgatcacc
      541 attttcagcg gccacggctc cagcaggtcg gcgaagattt ctcccaccac tgccacgaag
      601 gccagcgaga tggcgcgcca gtgcggacgc agcaatgccg caattccaaa agcctcggag
      661 cccacttttg tgtcgcccgt cctgtttttc atggattgcg tttcgaccca gcagcaactc
      721 aactgagtcg gagaagctga ccagagtgtg cgcatgccag gcgttccctc ctatcaggga
      781 aatcctgcat agccgggaat ttaacatccg tccgtaaccg gggaagcagg ccagcggttg
      841 gtctggtgta gactggcggt tatgttttgt ccgaaatgcg gcgcggaata ccggcctggc
      901 tacacatcct gcgcggattg ccacgtattt ctggtgcacg agcgctccgg cgcacaccaa
      961 cttgctcgcc tgcagcggac tgaagccgac cgtgatgtag agggaagccg tgcgaacgct
     1021 gacacgaact acgactatcc cggcgcagac cgcgtgcccg cggagccggg cgatccaaat
     1081 gaggatccgt tttgctcctt ctggaaaggc acggatgcgc gagtctgcgc cgagctctgc
     1141 acggtgttgg acgaggcggg gattccgcac aaaacgattc accggcagga tcatttgttt
     1201 aattttgcga atcagtcgcc ctacgagttg ggagtgccgg cgtcgctata tgaacaggcc
     1261 gagttggcgg tcaaagaagc gtttggtacc gacgaggaga ccggcgagga cgccgtaccg
     1321 ttgctgcccg cgcccgatgg cgagccggct gacttcgaaa agggcttgcg agatgcgatt
     1381 gccgatgcaa acgaatggct gggaccacgt tatccagagg acgctaccgt agaggtttgg
     1441 tccggcgagg acgcggaaaa ggacacaaaa gaaatgatcg agatgtcgct ccgcgaaaac
     1501 gatttgctgt gccgcagcga ggaagaaaac gggcggatca agctcctggt cttgccagag
     1561 gatgaggaaa gggcaagaca gattgttcgg gaaattgtgg aaggggcgcc actgggctga
     1621 tgtaaccgct ggccagggga cgtagggtca ggaagccaga gtccagccga ggacggtaag
     1681 cgttagcaga acgaaaagag aggcgaggct aatcgcgctg agcacacgac ctgtgccgag
     1741 caaggcgaga cgcgattcag cattgctgca gagcgcgatg atgaccagcg aaatggccag
     1801 ccaggccaag ccggagggca gtcccgggta agtatggatc agcgacatga aattctgcgg
     1861 aggctgcccc gcaggaatcc accaaggaag gaccggcgta acgcttgcca ctagaacaaa
     1921 gctgagaagt ttgcgcgtga gccagctttt gattttctgg cgcgacttgc ggcggacgaa
     1981 atagtcccag gcgagaatgc cggcggcgag cggcgcccag ccgtaaatgg cggctttgtc
     2041 ggtaagccac agccagctgc gaaagtagaa gccggcgtag tgtgcctgcg agaagcgcgg
     2101 cagcagcaga atcgccacca atccattgca aagaaccacc agaaagagcg tgcccagact
     2161 cgcgagttgg aacagctgat acgtaggcga ttcgccgcag cgcgtgcaga tcggatgctt
     2221 gggaaagatt tgcaggccgc agtgttcgca gtacatcgag acgctcctga gggggagatc
     2281 taccggctag cgagcttgcg agagcgaacg gagcgtatcg agttgcaagg cgcggcgcta
     2341 ctggccagaa gtacggttga agggaactgt ccgcgagcgc cagacaacgc acggaggaca
     2401 ggcgaacaca aagcgccgca tccagtgacc cgagggcgac ttgtgcggta ttgcggggcg
     2461 cgggtagaat tagcggactc cagcgcggca aaccttcgtt cgaaatttat tcctggatgg
     2521 gcgggactag atggcgcagc actatgacgt aattgtgatc ggcggcgggc acaacggcct
     2581 gaccaacgcc gcgtacctgg cgaaagccgg gaaaaaggtg ctggtgctgg agcggcgaca
     2641 cgtgttgggc ggcgcagcag tcaccgaaga gatcatcccg ggatttctat tcagcgaatg
     2701 ctcgtacgta gtatcgctgc tgcgcccgga aattatccgc gacttggatt tgccgcggca
     2761 cgggctggag attctgccgc tggatggaac gttcaccccg atgccgaacg gcgaccactt
     2821 gtggcgaatg aacgatcacg ggcggaccgt gcgggagatt cggcgtcatt cgcgggtgga
     2881 cgcggaggca tacgaagaat tctccaagat gatgacgccg atgtgccggt tcgtgaagcc
     2941 gatcctgtcg atgatcccgc ccgacccgac caccctgagg ccgaaagacc tgatgcagct
     3001 gaatttcctg atgcagcggt tccggtcgct ctcaagcgac gagcgctaca cgctgattca
     3061 gctgatgacc atgtcggcgg cggattttct ggaccaatgg tttgaaacag atgtgctgaa
     3121 ggcgaccatg tcggcttcgg ggattatcgg gacatttctc gggattcgct cgccgggcac
     3181 cgcgtatgtg ctgttgcatc attacatggg agaaattgac ggagcgttcc ggtcgtgggg
     3241 attcagccgg ggcggaacgg gcgcgatttc gaacgctatc gcggcggcgg cgcgggaagc
     3301 gggcgtggaa attcgtacca aggcgggcgt ggcgaaaatc attgtaaagg gcggccgcgc
     3361 ggcgggggtt gcgctgacga gcggggagga aatcagtgcg aatgtggttt catcgagcgt
     3421 cgacccgcac ctgacgtttg agaaatttct cgagccgaac gaactgccgg gcgactttct
     3481 ggaagaagtg aagcgctaca agttccgcgg gtcgtcgggg aaagtaaacc tcgcgctgga
     3541 cggactaccg gaattcaagt gcatgccggg agacggggca cacctgcgcg gggcattgtc
     3601 catttcgccg agcgtggagt acatggagcg cggctacgac gacgcgaagt atgggaattt
     3661 ttcgcggcgg ccgtatgtgg acatggtgat tccaagtctg actgatccgt cggtggcgcc
     3721 gccggggaaa cacgtgctct cgtgttttgt acaatacgct ccctacaagc tggcgaaagg
     3781 aacatgggac gatcagcgcg aagcgtttgg cgacaacgtg attgatacgc tgagcgagta
     3841 cgtcccgaac ctgaagaaaa tcattatcgg gcggcaggtt ttgacgccgc tggacttgga
     3901 gcgggagttc gggttgacgc agggcaacat ttttcagggc gaactttctc tggaacagtt
     3961 gttctttttg cggccggtgc cgggctgggc gtattacaaa acgccgattg agagtttgta
     4021 catgtgcgga tcggcgacgc atccgggcgg cggaattatg gcggcgccgg ggcggattgc
     4081 gagtcaagtg attctgaagg agtggaaggg cgggtcatcg agcaaaaata atccttcgcg
     4141 gtcgacggcg ccagccgcgg cccgggcggg gaactgacga tgcccctcgg acaaaaagta
     4201 gtgatcgttg gcggcggaca caacgggctg attgcagcat tttatctggc aaagggcgga
     4261 ttcaagccag tggtgctgga acgccgcgag atggtgggcg gcggcgcggt cacggaagag
     4321 ttccatccgg gatttcgcgc atcgaccttg gcgcacacgc tggggccgct gcgtgcggat
     4381 gtagcgcgtg acctgggcgt ggaaaaattc gattgccgga tttttgagcc tgacccgcgc
     4441 gtgttttcgc catcgctgga tggccgagga ttgctgtttt attccgacgc ggcgaagacc
     4501 gcgggcggca ttgcacggat ctcctcgaaa gacgccgaga aataccctca attttccgag
     4561 gcgctggaag aagtggccgg tgtttttgcg cagctgagtt cgatcacgcc gccagcgatt
     4621 gataagccaa cgccggaaga tttgtggaac ctcttcaaga ccgggcgcgg cgtgcgggga
     4681 ctgggcaaga agcgaatttt tgatttgttg cgctggggtc ccatggcggt ggcggacttc
     4741 gtggcggaat tttttgagac cgaactgatt cgggcaacga tcgcggcacg aggcattttt
     4801 ggcacggcgc tagggccgtg gtcggcaggc tcgacggcgg tgctgctgat gcgcgcggcg
     4861 gcggatgcta acccggtagg ctcggcggca tttgcacgag gcggacttgg taatttttcg
     4921 cgggcgctgg cggaagcggc caagcaggcg ggcgctgaaa tccgtaccga cgctgaggtg
     4981 cagcagattc gcaccaagga tggagcggtc gtcggagtcg tgctgcgcga cggcgaagag
     5041 atcgccacgg aagcagtggt gtcgggcgtg gatccgaaga ggacgttttt tggattgctg
     5101 gacccaacgc aactcgatcc gcgattcgcc cagcgcatga aaaatgtgcg tatgaagggc
     5161 acggtagcga aagtgaacct ggcgctgggc gatctgcctt cgttcccttc gttggcggat
     5221 gcgatcgggc aggacggtta tttgcgcgct ctttccgggc gcattcatat cgggccggag
     5281 atcgactatt tggaacgcgc gttcgacgtt tcgaagtatg gcgaatttcc cagcgcgccg
     5341 tggctcgacg tgacgcttcc gacgattctg gacccttcct tggcgccgga tgccaagcat
     5401 atactttcgg cgtacgtgca gtttgcgccg tttacgctgc gcgatggcag ttgggattcg
     5461 cgacgcaagg aactcggcga agtggtgatc aaaacactgg cgccgtatgc gccgaatctg
     5521 ccggggctgg tggaaggaat gcaggtgatt acgccagcgg acctggaaaa gagttacgga
     5581 tttaccggcg ggcatatttt tcatggcgag ctggcgctgg accagttgtt tacgatgcgg
     5641 ccggcgctgg actgggcgcg ctacaaaact ccggtacgcg gactattcat gtgcagcaac
     5701 ggcacgcacc ctggcaacgg gttgaccggc gcgagcggag ccaacgccgc aagagaaatt
     5761 attcacgaac tgcgatgagc gcgatcaaac gcgaaaagct gcaccaactc aagcaacgcg
     5821 aagaagccag atttattgcg gagcatccga agtcggcgga gctatacgcg cgcgccaagc
     5881 attcattgct cggtggcgtg ccgatgaact ggatgaacaa gtgggctggc gcgtttccag
     5941 tgtttgtggc taaggccaag ggcgcccatt ttacagacgt ggacggacac gagtacgtgg
     6001 acctgtgcct cggcgatacg ggcgcgatga ccgggcactc gccggatgtt gtggccgatg
     6061 cgattttgaa gcgggtgcgc gaaggcatta cgctgatgct tccgacggaa gattcactgt
     6121 gcgtcgggca ggagctgaag caccgatttg ctttgccgta ctggcagttt gcgcttacgg
     6181 ccaccgacgc gaaccggttt gcgattcgca ttgctcgcga gattacccag cggccgaaaa
     6241 ttttggtgta ccactactgc tatcacggga cggtggacga atcgttcgca tccctgcata
     6301 acggcgttgt cgggccacgc cgcgggaaca ttgggccgcc agtgaacccg gcggagacga
     6361 cgcgcgtcgt tgagttcaac gatctgaatg cgctggaaga tgcgctggcc gaccacgacg
     6421 tagcggtgtt gctggctgag ccggccatga ccaacgttgg aatcattttg cccgacgacg
     6481 gctattggaa agcggcacgg gcgctgacac gcgaatatgg aacgttgttc atcgcggatg
     6541 aaacacacac gatttgcgcc ggtccgggcg gctgtaccaa ggaatggaag ctcgatccgg
     6601 attttgtggt cttcggcaag cccatcggca gcggcattcc gggttcgacg tacggcgtga
     6661 cggaagaagt agcaaagcga atttcagcgc gcatcgaact ggaagactgc gacgtcggcg
     6721 ggatcggcgg cacactcgcc ggcaacgcgc tttcgctcgc cgccatgcgc gccacactgg
     6781 aaaacgtcct cactgcggaa gcctacaaaa cgatgattcc tctggcgcaa cgcttcaatg
     6841 acggcgtcgc ggcggaaatc aaaaagaacg cgctcccgtg gaacgtgatt cagttggggg
     6901 cgcgcgccga gtatcacttt acgccgaagc ctccgcgcag tggcggagaa tcggcggctg
     6961 cggcggattt tgaactggag cggttgctac atttatacgc gctgaaccgc ggcgtactcc
     7021 tgacgccttt ccacaacatg gcgctgatgt gccccgcgac gacagccgcg gacgtagacc
     7081 tgcatacggc gatatttgct gaggccgcag cagaactagt ggcttaagcg ctaaaataga
     7141 gaattgcagc aaggagacgc aacatggcca ccgccgaagt aaaactgcaa aaaccagaat
     7201 tcgccaacga gccgttcgtg gatttttcga agctcgagaa tcgcgctgcg atggaagccg
     7261 ccttgaaaaa ggtcgccgct gaatttggcc gtgagtatcc catgtatatc ggcgggcaga
     7321 aagcgacgac gacggaaaag atgcactcca ccaacccgtc gcacccgtcg caggtgatcg
     7381 gcatcgtgca aaaagccagc gcggagcagg ccaaacaagc catcgaatcg gcgcacggct
     7441 atttcgatac ctggaaaaag gttccggccg agcagcgcgc ggaaattctc tttcgcgccg
     7501 ccgccatcat acgccagcgc aaattcgaaa cttccgcgct gatctgctac gaagtcggca
     7561 agtcctggcc cgaagctgac gccgacactg ccgaaaccat cgatttcctc gaatactacg
     7621 gccgcgaaat gttgcggctc gccggcccgc acaaagttgt cccttatcag aacgaacgca
     7681 attttatggt gtatatcccg ctgggcgcgg gtgccgctat tccgccgtgg aattttccgg
     7741 ccgctatcgc aaccggcatg accgccgcgg cgctggttgc gggcaacacc gtagtcttga
     7801 agccctccga agaatcgcca gtcatcgccg cgaagatggt cgaaattctg taccaagccg
     7861 gcattcctaa agaggcgctg cagttcatca ccggcgaggg cgaagttgcc ggcgacgcac
     7921 tggtcaagca tccgaagact cgcttcatcg catttaccgg atcaaaagac gttggcctgc
     7981 tcatcagcga agccgcgggg aagcgccagc cgggtcagtt gtggatcaag cgcgccgtgc
     8041 tggaaatggg tggcaaggac gcgacggtag tcgacgaaga agcggatgtc gatgccgccg
     8101 tggaaggagt ggcgcaagcc gccttcggct atcagggaca aaaatgttcc gcctgttcgc
     8161 gcgccattgt ttcggaaaag gtttacgaca cgttcgtgca gaagctcgtc gatcgtgcga
     8221 aaaaagtcac cgttggcccc agcgaggatg cagcaaactt tatgggcccc gtggtcagcg
     8281 aaggcgcaat gaacggaatt ttgaactaca tcgaggttgg aaaaaaggaa ggtaaggtag
     8341 tgctaggcgg cggccgcgca cccggagatg gctactttgt gcagccgacg atcatcgcgg
     8401 acgtaagccc gaaagcgcgc atcgcgcagg aagagatttt cggtcccgtg ctcgcggtga
     8461 tcaaagccaa ggattttgat gaagcgctac aaatcgccaa tgactcggaa tttggcttga
     8521 ccggcgcggt ctattccaaa aacagagcta agctcgagaa agccgaagaa gcatttcacg
     8581 tcggcaatct gtacctgaac cgcaagtgca ccggcgcgat ggtaggcgcg cacccctttg
     8641 gcggattcaa catgtccggc acggattcaa agaccggcgg caaggattac ttgctgcact
     8701 tcctgcaggc caagtcgatt gcggaaaagc cgcagtaagc ggcgactcag cggctggccg
     8761 ccgctgtagc gagctcgcgc caggtaatca gcttgacgcg ctgctgttca agcagcgagc
     8821 ggaattcctt gctcatgaag aagtcgaagt cgcgttgccg ccaggcggcg ccccaggtcg
     8881 cgcgctcgcg cgtggccgcg cgcagttcct catcggccaa tcctacgtgg atcacgaatt
     8941 ctgtgactcc gggctgtagg tttttcaccg cgtcgcgata gaaatccgcc cacttttccg
     9001 cgggaacgtc agggctgatg gtcaccgtat gatcgagtat cagatctcgc ggacctaggc
     9061 tggcctcgag gtagggatgt tcggagaacc agtcgcgtgc cacaaagatt ggcacgccat
     9121 attcgtggcc cagccgcaca aaaacctgga agatctcctt gccgttctca atcaagcggt
     9181 attgatgcga atcgaaatgg cttgggcgta cgcccatggc ccgtgcgcgt tcaatctgcg
     9241 cgcgtagttc ggtttcgaca tcgcgcgggt caatccgggg atgttgatcc cagtcatgat
     9301 gaaagtatcc gttgctgtcc accaggctgg gaacgctgtt gcgcgctgcg accgggcccc
     9361 agcggtaata cacgcgttcg ctggtaagcg tgagatgcaa gccgaaatcg acctcgggat
     9421 gagacctcgc gtagtcggcc gcctctggaa accaggggca gggaaccatg atgctggcgg
     9481 aattgaccgc gccagattcg actgcctgga aagtggcagc gttgacggcg tgcgtttcgg
     9541 cgacgtcatc cgcgtgaatg atgagcagct tggcgtcctt gggatagccg agcttttcgg
     9601 caagagtctg cgcgttgtta gtcgcaggaa aaagcgcaac cagcacggcg agagagagcc
     9661 accgcagttg gagtttcatg ctttgcttcc ccgagtaggc gaaaccattt tgaggacgcg
     9721 gaaaatcgga tagctagcga cgagaatgcc aaaagcccac ggcgtgttta cgcggtctgt
     9781 gtagagcgcg cccgcgagga aaagtaagga tgctgccagc gcaacgccag tcgtccaggg
     9841 atagccccag gcgcgataag ggcgaggcag atcaggtgct cgcgagcgca aacggaacgt
     9901 ggagatgaac gaaagcgtgt agttcgcaac gaagaaaaaa gccagcatat caatcacgcg
     9961 gatgaatttc tggaaaatgc aaaaagagat gccgaccagc gtgctcagga ccatggcgag
    10021 gaccggcgtg ccgcccgaat tgacgcggga tgcggcgcgg aaaaacaggc cgtcgctgct
    10081 catggcgtag agcgtgcggg tggagaatag caggcaagcg ttgatgcagc tcaatagaga
    10141 aatcagcatg atggagcgga tgacagggtc gccgtatgtg ccgaagatac gcgcggcagc
    10201 ggtgccgagc gcgaatccgt tgccaacgat ttcgttcatc ggcaggacgt aagcgacggc
    10261 cgcgctgaga agaagataga tcccgatgat ggagaatacg ctgccgaaaa tggcgcgtgg
    10321 aacgtcgcgg cctggatctt taacttcttc gccaaagtag atgacaccgt cccagccgtc
    10381 cacggtgtag atgacttttt gcagcgctag catgaaggca gtggcgagaa gccagccgcc
    10441 gggaagcggc aacggtgtgc tggcagcaac ggcggcggcg tgccaacttc cgccaaacac
    10501 aaagcaggcg atgaccagga cgaggaatgc gccggtcttg agtgcggcgg tgatcaactg
    10561 agcggcgctc ccccacttga tgccacgcca ctgcaggaca gcgaagccca gcaaaataat
    10621 gacggcgatg gttgcgtctc taccggcgag cgcaggaaaa agtactcccg tgtaattgcc
    10681 gattacgagc gcgaccaccg cgttcgtgcc gcaggtggag atccagtcgc tccagcccac
    10741 gacgaagccg gcgtattcgc cgagcgcgcg acgcgaataa ttgtattgtc cgccgctgcg
    10801 cgggatcgca gcaccgagct cggcaagaga cgaagcgccg aaaatggcgt agagaccgcc
    10861 gagaatccag actccgagaa acagccacac attcggtaac tgggcggcga tgtagccctg
    10921 cgctgcaact atgccggcgg caatagtgtt gccgatggcc gcggcgatcc cgaaccacat
    10981 gcccagcact tgcaacagcc gcccgcgcga cggacgggcg gtacctgcgc tggaagatgc
    11041 atctaaagta gtggacaatg aacgtctcag actttctgag atgcgccatg atactggcga
    11101 atttcgaaaa agcaaatcac acacgatcgc cagggaatgc aatcggcatg ttcaccgcag
    11161 gaggttctgc tgctcggttg aggtgagttg gtggaaacga agcgcacgtc cgaacctgga
    11221 ccgcagctaa cgacgcagtt ggtcacagac gtcccgacgg aaaccgtcga tgtgtcggtc
    11281 gtcattccct gcctgaacga agcgaattcg ctccgcgtgt gcattgaaaa agcgcaaaaa
    11341 gcatttaagg aggcgcggct gcgcggcgaa gtcgtcgtag ccgataacgg atcgaccgac
    11401 ggctccatcg aaattgccca gacagccgga gcgcgcgtga ttcccgtcgc gcagcgaggt
    11461 tacggatcgg cactgaaagc cggcattcgc gcggctcgtg gcgccttcat cgtcatgggt
    11521 gacgccgacg acagctacga cttcagcgaa gtcccccgct tcgtcgaaga gtggcgcaag
    11581 ggcagcgaag tggtcatggg caaccgcttc cgaggagaaa tcaagccggg cgccatgccg
    11641 tggcaccaca aatacatcgg aaatcccggc ctcaccgcta tgctcaacgt gctcttccgt
    11701 tccggcatcg gcgattcgca ttgcgggatg cgcggattta cgcgcgacgt cttcgagcgc
    11761 atggatttgc gcagcagcgg catggagttc gcctctgaat ttgtgatcaa ggccgcgcaa
    11821 atcggcgcca agacttgcga aattcccatc acgctttgga aagacaagcg cggccgtccg
    11881 ccgcacctgc gcagttttcg cgacggctgg cgtcaccttc gctttatgct gctctatgcg
    11941 ccgaactggt tgtttctgct tccgggcggc tcgctgatgc ttttgggact tgcgctagtg
    12001 ttttggcttt tgccggcgcc gcgcaccatt ggc
//
LOCUS       DASTPM010000681         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1206935, whole genome shotgun sequence.
ACCESSION   DASTPM010000681 DASTPM010000000
VERSION     DASTPM010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206935"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VFR42_12025"
     CDS             <1..1666
                     /locus_tag="VFR42_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0369935.1"
                     /translation="RRINVKKATICLVMTGILGSLAGVGVRPALAQGTSAGASSVSRT
                     TKAVNFRRAGGSTKLDFHGTDLMPTASGEAKVESKSNRIEIDAKLDGFEEATKFGLEY
                     LTYVLWAVSPQGRAVNLGEVVLNHGNGHVKAISDMQTFGMIVTAEPYFAVQQPGNVVV
                     LENVIPADTLSKVENIDARYELLARGVYSSSNTKIENAIFGIDRKTPLELFEARNAVR
                     IARIALADKYAGSIMSKAEQQLKGAEDVYRSGRDKKSVDAAAREVVETAEEARVMAVK
                     QKAEEDAQASVAAEKRAALEREAKARADAAAELKRRQEADQARAAAEAAKAEAERMKQ
                     EAEQASQDAVRQKQEAEQARAAALQQQQVAQAAAEQAARDRAAAQSQQRAAEAEAEKA
                     RQAAAQAEAEKAQLRSQLLSQLNSILQTRDSARGLIVNMSDVLFDTGSYTLKPGAREK
                     LAKISGIVLAHPGLNLQIEGHTDSVGTDEFNQSLSERRADSVRDFLAEQGVPGSAISA
                     RGFGKTQPVASNDTAEGRQRNRRVELVVNGDAIGGGNANAAVSSNQ"
     gene            complement(1744..>2181)
                     /locus_tag="VFR42_12030"
     CDS             complement(1744..>2181)
                     /locus_tag="VFR42_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEU0369936.1"
                     /translation="AVALDSNGHLFAATSTGGTCCKLPGRVGDSPLIGCGCYADAESG
                     AVSCTGYGEAIMKVVLAKTAVDLLRHPIGTDKKPIPSIQPKLTIPDVANLAAREAVSV
                     LAARTRATGGLILLDREGNPGFAFNTPRMAYGYVANGSFVIGI"
BASE COUNT          480 a          581 c          778 g          342 t
ORIGIN      
        1 gaggagaatt aacgtgaaaa aggcgacgat ttgcctggta atgacgggaa tcctgggctc
       61 cttggctgga gttggggttc ggccggcgct cgcgcagggg accagcgctg gcgcgtccag
      121 cgtctcgcga acgaccaagg cggtaaattt ccggcgtgct ggcgggtcca caaaactgga
      181 ctttcacggc accgacctga tgccgaccgc gagcggcgaa gcgaaggtag agagcaagag
      241 caaccggatt gaaatcgacg cgaaattgga cgggttcgaa gaggccacca agtttgggct
      301 ggaatacctg acgtatgtgc tgtgggcggt gtcgccgcag gggcgcgcgg taaacctggg
      361 cgaagtggtg ctgaatcacg gcaatggcca cgtgaaggcc atcagcgaca tgcagacgtt
      421 cgggatgatc gtgaccgcgg aaccctactt cgcggtgcag cagccgggaa acgtggtggt
      481 gctggagaac gtgattcccg cggacacgct gtcgaaagtc gaaaatatcg atgcgcggta
      541 cgagttgctg gcgcgcggag tctattcctc ttcgaacacc aagattgaga acgcgatttt
      601 tggaatcgat cgcaagaccc cgctggagct gtttgaggcg cggaatgcgg tgcgcatcgc
      661 gcggattgcg cttgccgaca agtacgccgg atcgatcatg agcaaagcgg agcagcaact
      721 gaagggcgcc gaggatgtgt atcgctccgg gcgcgacaag aagtccgtgg atgcggcggc
      781 gcgcgaagtg gtggagacgg ctgaagaagc gcgggtaatg gccgtgaagc aaaaggccga
      841 ggaggacgcg caagcgtcgg ttgcggcgga aaagcgagct gcgctggaac gcgaagcgaa
      901 agcgcgcgca gacgcagcgg cggagctaaa gcgccggcag gaagcggacc aggcgcgggc
      961 agcggcggaa gcggcaaaag ctgaagccga gcgcatgaaa caggaggcgg agcaggcctc
     1021 gcaggatgca gtccggcaaa agcaggaagc ggagcaggcg cgtgcggcgg cattacagca
     1081 gcagcaggta gcgcaagcgg cggcggagca agcggcgcgt gaccgtgcag cggctcaatc
     1141 gcagcagcgc gcggcggaag ccgaggctga gaaggcccgg caggccgcgg cacaggcgga
     1201 agcggaaaag gcgcagcttc gttcgcaatt gctttcgcaa ctgaattcga tcttgcagac
     1261 gcgggactcc gcgcgcgggc tgatcgtgaa tatgtccgac gtgctgtttg acaccggaag
     1321 ctacacgctg aagccgggag cgcgcgagaa actggcgaag atttcgggca ttgtgctggc
     1381 gcatccaggc ttaaacctgc agatcgaagg gcatacggat agcgtgggta cggacgagtt
     1441 caaccagtcg ctttccgagc ggcgtgcgga ttccgtgcgc gattttctag cggagcaggg
     1501 cgtgccgggt tccgcgatca gcgcgcgcgg atttggcaag acgcaaccag tcgcttcgaa
     1561 cgatacggcg gaagggcggc aacgcaatcg ccgcgtcgaa ctggtggtga acggcgacgc
     1621 gattggtggc gggaacgcga acgcggccgt gagttcgaat caatagcatc tgctctctgc
     1681 cacagggcat tccgagagta gacgacccca tgcagcggac aggcaagaat ggctgtcctg
     1741 cggtcagatc ccgatcacaa acgagccgtt ggctacgtag ccgtacgcca tacgcggcgt
     1801 gttgaaggcg aagcccgggt ttccttcgcg gtcgagaagg atcaggcctc cagtggcgcg
     1861 cgtgcgcgcg gccaacacgc tgactgcttc gcgagcagcc agattggcaa cgtccgggat
     1921 ggtcagcttc ggttgtattg atgggattgg cttcttatcg gtaccgatcg gatggcgcag
     1981 caagtccacg gctgttttcg ccaataccac tttcatgatg gcttcaccat aaccggtgca
     2041 agaaacggcg ccggactccg catcggcata acaaccgcaa ccgattagcg gggagtcccc
     2101 tacgcggccg ggaagcttgc agcaggtgcc gccagtgctg gtggccgcga acaaatggcc
     2161 attgctgtcg agagcgacag c
//
LOCUS       DASTPM010000682         5299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1178316, whole genome shotgun sequence.
ACCESSION   DASTPM010000682 DASTPM010000000
VERSION     DASTPM010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178316"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VFR42_12035"
     CDS             <1..1314
                     /locus_tag="VFR42_12035"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU0369937.1"
                     /translation="KITLKNGMTVLLMEKRGVPMVSFAAIVKASSVADPVGLEGLASS
                     TAGLLRKGTTKRSAQKFAEDLDFIGASFSADASADFTAISAECLSKDLDQGLDLLSDA
                     MLHPAFPQSEVDKLLSQDIDGVKAAKDEAESVALTYYYGYLFDEHPYARPEGGDELSL
                     ARIKRDAIVKFYQANYTPGDTILAVAGEFKASQMKAKLEEVFGAWTAQGATPPVLSAP
                     LPVKGRRLLLVDKPDSTQTFFVIGNVGMAVNDPDRVAIRVVNTIFGGRFTSQLNEALR
                     VESGLTYGAQSFFDSKKQPGAFAIYSYTKNESTVQAIDLALHILDKLHKNGVTAEELA
                     SAKAYIKGQFPPSIETSGQLARRIASNEFYGLNDDEINQLEARIDAVTLPVAKQAIEK
                     HFPADNLVFMLIGKAAEIRPAVEKYAPQQDARKISEPGFWPPPGK"
     gene            1405..4734
                     /locus_tag="VFR42_12040"
     CDS             1405..4734
                     /locus_tag="VFR42_12040"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0369938.1"
                     /translation="MKVRFAVLLAMLLVAANFACAADDSPLLATEPTISKTEIVFAYG
                     GYLWSVPRAGGEARQLTTGGHESSPYFSPDGKWIAFTGQYDGNEDVYVMSAEGGEPRR
                     LTWHPANDGPVGWTPDGRRVAFISTRDAWADVTRIYTVPVEGGVPEVLPMWRAFEGSY
                     SPDGKSFAYVPNLKWQEAWKRYRGGQTTPIYIVRLSDLHLEKVQRENSNDANPVWVGD
                     TVYFLSDRNGPVTIFAYDVKSKQVKRVLENQGLDVKSISAGPDALVYEQFGGIYTLDT
                     NSGKSERVSIHVAGDLPATRPHFEKVADKIGNANISPTGARAVFEARGEILTVPAEKG
                     DIRNLTRTTTLVERDPAWSPDGKWIAYFSDESGEYALHLKDQSGLGEVKKINLGNPPS
                     FFYSPNWSPDSKKISYIDKRLNLWYVDIEKGTPVKVDADLYEDPTYALDPAWSPDSKW
                     LTYSRYLQSHFRAVFVYSIESAKSSQITDGLGEARFPVFDKNGKYIYFGASTDFGLSL
                     GWLDLTSFQHPVSRSIYAVVLKKGEPSPTAPESDDEKIAKAEEKKSDEKSKDTKASDK
                     DKKGSEKKDAEKKDADKKDDDKKEEKVVVTIDLDGIGQRIVSLPIKGANYSGLTPGKP
                     GILYLIEGPALPSISGPPAFTISKFDVTTRKTEPFLSGVSGGAFSANGEKVLYRQGES
                     WFIAATAAAPKPGEGALKLDEMEVYVDPRAEWNQMYHEVWRIERDFLYDPHWHGLDLA
                     AAEKKYAPYLKGVGGRGDLNHLFTEMLGEITIGHMFIGGGDFPKAPKVNGGLLGADYT
                     IENGRYRFSRIYNGENWNPDLRAPLTQPGVDVKTGDYLLAVNGVDVKPATEVYRYFEN
                     TAGKQVKIKVGPNADGKDARETTVVPLGNDFPLRNRAWEEDNRRKVDELSGGEIAYVH
                     VPDTATGGYLNFNRFYFAQVGKQAAIIDERYNHGGLIADYIIDLLERPLRNCAISREG
                     GKFCSPLAQIYGPKTMIINEMSGSGGDALPWMFKQDKIGPLVGTRTWGGLVGIYNYPP
                     LMDGGGVTAPRVAIYGLHGEWEVENHGLAPDIEVENDPVSVAAGHDAQLEKAVEVTLE
                     ALKKHPVVIPDHPSYPNYHQK"
     gene            4833..>5299
                     /locus_tag="VFR42_12045"
     CDS             4833..>5299
                     /locus_tag="VFR42_12045"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU0369939.1"
                     /translation="MPELPEVETVARGLQSAVAGRRILTVTLRKSDFIDDPAALEREL
                     PGRQIERIERFGKFMLLRLAAAIAQSGPELPPGEDSQKIEPIAEALLVHLGMTGHLAP
                     HFPYQPLTKHTHVTFELDDGRELRYTDARRFGRMAYLAGAALAAELERFGADPL"
BASE COUNT         1201 a         1376 c         1669 g         1053 t
ORIGIN      
        1 aaaattacgt tgaaaaacgg catgaccgtg ctgctgatgg aaaagcgcgg agtgccgatg
       61 gtgagttttg cggcgatcgt gaaagcaagc tcggtagctg atcccgttgg tctggagggg
      121 ttggcttcca gcaccgcggg actgctgcgc aagggcacga ccaagcgcag cgcgcaaaaa
      181 ttcgcagaag acctggattt catcggcgcg agttttagcg ctgatgcgag cgcggacttt
      241 accgccattt ccgccgagtg cctgagcaaa gatttggacc agggattgga tctgctgagc
      301 gacgcgatgc tgcatccggc gtttccgcaa agcgaagtcg acaagctgtt gtcgcaggac
      361 attgacggag tgaaagcggc caaggatgaa gcggagtcag tggctttgac gtattactac
      421 ggatatttgt tcgacgagca tccctatgcg cggccggaag gcggcgatga actttctctg
      481 gcgcgcatca agcgcgacgc catcgtgaag ttttaccaag cgaattacac gccgggcgac
      541 accattctgg cagtcgccgg cgaattcaaa gcgtcccaga tgaaagcgaa gctggaagag
      601 gtttttggcg cttggacggc gcagggcgcg actccccccg tgcttagcgc tccgctgccg
      661 gtaaagggca ggcggctttt gttggtcgac aagcctgatt cgacgcaaac gttctttgtg
      721 atcggaaatg tcggcatggc ggtgaacgat ccggatcgcg tggcaattcg cgtggtgaat
      781 accattttcg gcgggcgctt tacttcgcag ctgaacgagg cgctgcgcgt ggagtccggc
      841 ctgacttatg gtgcgcagtc atttttcgat tcgaagaagc agccaggcgc atttgcgatt
      901 tacagttata cgaaaaatga gagcaccgtg caggcgattg acctggcgtt acacatcctg
      961 gacaaattgc acaagaacgg cgtgacggcg gaagagttgg cttcggcgaa agcctacata
     1021 aaggggcagt ttcccccttc gatcgaaacg tcgggccaac tggcgcggcg catcgcaagc
     1081 aacgaatttt atggattgaa cgacgatgag atcaatcaac tggaagcgcg catcgatgcg
     1141 gtgacgttgc cggtggccaa acaggcgatc gagaagcatt ttccggcgga caatctggtg
     1201 ttcatgctga tcggcaaagc ggcggagatt cgtccggccg tggaaaagta tgcgccgcag
     1261 caggatgcgc gtaagatttc cgagccggga ttctggccgc cgccagggaa atagtggtcc
     1321 gtcggcagtt tgcggttatc cgtgagaaca gattcagagt gcggcatagc gtttcaggcc
     1381 atgccgcgtg tggggggagg atccatgaag gtgcgtttcg ctgtgctgct tgccatgttg
     1441 ctggttgcgg cgaattttgc ctgcgcggcg gacgattcgc ctctgttggc gacggagccg
     1501 accatcagca agacggagat tgtgtttgca tacggcggtt acttgtggag cgtaccgcgc
     1561 gcgggcggcg aagcgcgcca actgactacc ggcggccacg aaagcagtcc gtacttctct
     1621 cccgatggaa aatggatcgc gttcaccggg caatatgacg gtaatgaaga tgtctacgtg
     1681 atgtccgcag aaggcggcga gccgcggcgg ctgacctggc acccggcgaa cgacggtcct
     1741 gtgggatgga cgcctgatgg caggcgcgtg gcgtttatat cgacacgcga cgcgtgggcg
     1801 gatgtcacgc gaatctacac cgtaccggtg gaaggtggag tgccggaagt gctgccgatg
     1861 tggcgcgcat ttgaagggtc gtattcaccg gacggcaaga gcttcgctta cgtgcccaac
     1921 ttgaaatggc aggaggcgtg gaaacgctat cgcggcggac agaccacgcc gatttacatt
     1981 gtgcggttga gcgacctgca tctcgagaaa gtgcagaggg aaaattccaa cgacgcaaac
     2041 ccggtgtggg tgggcgatac ggtttacttt ttgtcggacc gcaacggacc tgtgacgatt
     2101 tttgcttacg acgtgaagtc caagcaagtg aaacgggttt tggagaacca ggggttggac
     2161 gtcaagtcta tttccgccgg gccggatgcg ctggtgtatg agcagttcgg cggaatttac
     2221 acgctggata cgaacagcgg caagtccgag cgggttagca tccacgttgc gggcgatctc
     2281 ccggcgaccc ggccgcattt tgaaaaggtc gcggacaaaa tcggcaacgc caacatttcg
     2341 ccgaccggag cgcgcgcggt ctttgaggca cgcggagaaa tcctgaccgt gccggcggaa
     2401 aagggcgaca ttcgcaatct gacgcgcacg acgactttag tcgaacgcga tcccgcttgg
     2461 agtcccgacg gaaagtggat tgcttatttt tccgacgagt ccggcgagta tgcgctgcac
     2521 ttgaaggatc agagcggcct cggcgaagtg aagaagatta acctgggcaa tccgccatcg
     2581 tttttctaca gcccgaattg gtcgccggac agcaagaaaa tttcctacat cgataagcgg
     2641 ctgaacctgt ggtacgtgga catcgaaaag ggcacgccgg taaaagtcga cgcagatttg
     2701 tacgaggatc cgacgtacgc actcgatccg gcatggtcgc cggacagcaa gtggctgaca
     2761 tattcgcgtt atttgcagag ccatttccgc gcggtgtttg tgtattcgat agagtcggca
     2821 aaaagttcgc aaattaccga cgggctcggc gaagcgcgtt ttccggtgtt cgacaaaaac
     2881 ggaaagtaca tttatttcgg cgcgagcacg gactttggct tgtcgttagg ctggctggat
     2941 ctgaccagtt tccagcatcc tgtttcgcgc agcatttatg cagtcgtctt gaagaagggc
     3001 gagccttcgc cgacggcgcc ggaaagcgac gacgagaaga tagctaaggc ggaagagaag
     3061 aagtccgacg aaaaatccaa agataccaaa gcaagcgaca aggacaagaa aggctccgag
     3121 aagaaggatg ctgagaagaa agacgcggac aaaaaagatg acgacaagaa ggaggagaag
     3181 gttgttgtca cgatcgatct cgatggaatc ggccagcgca tcgtttcttt gcccatcaaa
     3241 ggggcgaact atagcgggct cacgccggga aaaccgggca ttctttatct gatcgagggg
     3301 cccgcgctgc cgtcgatcag cgggccgccg gcattcacga tcagcaagtt tgatgtgacc
     3361 acgcgcaaga ccgagccttt tctgagcgga gtgagcggcg gcgcattttc cgcgaatggc
     3421 gagaaggtgc tgtaccggca aggtgagtcg tggtttatcg cagcgactgc cgccgcgccc
     3481 aagcccggcg aaggcgcatt gaagctcgac gaaatggagg tgtacgtcga tccgcgcgcc
     3541 gaatggaacc agatgtacca cgaagtatgg cgcatcgagc gtgattttct ttatgacccg
     3601 cactggcatg gactcgacct tgccgccgca gagaaaaaat acgctcctta tttaaaagga
     3661 gtcggcggcc gtggcgattt gaaccatctg ttcaccgaaa tgcttggtga aatcaccatc
     3721 gggcatatgt tcatcggtgg tggcgatttc ccgaaagcgc ccaaagtgaa cggcggcttg
     3781 ctcggcgcgg attacacaat tgaaaacggg cgctaccgct tttcgcgcat ttacaatggc
     3841 gaaaactgga accccgattt gcgcgcgccg ctgacgcagc ccggcgtcga cgtaaaaaca
     3901 ggggactacc tgctcgcggt gaacggagtg gatgttaagc ccgccaccga ggtgtatcgc
     3961 tacttcgaaa acaccgccgg caaacaagtc aaaatcaaag tcggccccaa cgccgacgga
     4021 aaagatgcgc gcgaaacaac cgtcgtgcct ctgggcaacg actttcctct gcgcaaccgt
     4081 gcgtgggaag aagacaatcg ccgcaaagtt gacgagctga gcggcggcga gatcgcctac
     4141 gttcatgtgc ctgatacggc gacgggcggc tatctcaatt tcaaccgttt ttactttgcg
     4201 caggtcggca agcaggcagc cattattgat gaacgctaca atcacggcgg cttgatcgcg
     4261 gactacatta ttgatttgct ggaacggccg ctgcgcaatt gcgcgatttc gcgcgaaggc
     4321 ggaaaattct gctcgccgct cgcgcaaatt tatggaccga agaccatgat catcaacgaa
     4381 atgtccggtt ccggcggcga tgcgctgccg tggatgttca agcaggacaa aatcgggccg
     4441 ttggtcggca cgaggacgtg gggcgggctg gtcggcatct acaattatcc tccgttgatg
     4501 gacggcggcg gcgttaccgc gccccgcgtg gcgatctacg ggctgcacgg cgaatgggaa
     4561 gtggaaaatc acggtctcgc gccggatatt gaagtggaga atgatccggt gtcggtggcg
     4621 gcggggcacg acgcgcaact cgaaaaagcg gtcgaggtca cgttggaggc gctcaagaag
     4681 catccggtcg ttatcccgga tcatccgtcg tatccgaatt atcaccagaa gtagcagcgc
     4741 gggtagttac aaccgaaacg aattgaatgc gggccatttg gcgagggccc aaacgttgcc
     4801 cgaagtttgc gctgtgctat tttcatttaa atatgccgga acttcctgaa gtagaaaccg
     4861 ttgcgcgcgg cttgcagtcc gccgtggctg gacgtcgtat attgacggtc acgctgcgca
     4921 agagcgattt catcgacgat cccgcggcac tcgaacgcga attgccgggg cgacagatcg
     4981 agcgcatcga acgcttcggc aaattcatgc tgctgcggct cgccgctgcg atcgcgcaat
     5041 ctgggccgga attaccaccg ggcgaagatt cgcagaagat cgagcctatt gccgaagcgt
     5101 tgctcgtcca cctgggtatg accggccacc tggcgccgca ctttccgtat cagccgctaa
     5161 caaaacatac gcatgtgaca ttcgaattgg acgacggccg tgagctccgc tacaccgacg
     5221 cacggcgctt cgggcgcatg gcgtatcttg ctggcgctgc gctggccgcc gaactggagc
     5281 gcttcggcgc cgatccgct
//
LOCUS       DASTPM010000683         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1005187, whole genome shotgun sequence.
ACCESSION   DASTPM010000683 DASTPM010000000
VERSION     DASTPM010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005187"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFR42_12050"
     CDS             <1..343
                     /locus_tag="VFR42_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0369940.1"
                     /translation="SMSRVGEILVIDPATITRSVRLLQNDGLLEIAPRGLRRQRLLTL
                     TSKAEKVLAIAVPLWREAQARFIASLGGKWKEFQDELEHAAEVAVSLEIADTAADTAA
                     QRNSPRQKNRA"
     gene            complement(461..1330)
                     /locus_tag="VFR42_12055"
     CDS             complement(461..1330)
                     /locus_tag="VFR42_12055"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0369941.1"
                     /translation="MILITGASGTAGREVLQAVAKSGAKHRAMYRSAVEATKAPPGTE
                     TVIADFAKKEALGSALRGVSSVYLVCGPVPELVELESNMIDACVEAGVTHVVLNSALG
                     AGDYDKSFPSWHRKVEDKLRGTKLSWTILRPNSFHQNTVTYYAPSIRAQGVFYSSLGN
                     ARTSYIDVRDVAGAVARTLSGREHSGKIYELNGPEALTANEVAEEIAKHSGRPVKYVD
                     IPMEAQRKSMLDLGMPEWQVTALLDLQQYYVNGKGGEVDDVLQRVIGRPPITMEQFLA
                     ENAAAFRAQSATA"
     gene            1460..1852
                     /locus_tag="VFR42_12060"
     CDS             1460..1852
                     /locus_tag="VFR42_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0369942.1"
                     /translation="MKGHGNGNQKNDFGCPVQGTINVLSGKWKGLAVWHLGFGAKRFA
                     ELRDLLPGVSEKVLTSQLRQLESDGVVRRRVKPTSPPQVTYSLTKAGGELIPMMSDLC
                     HWATKHLGIPPMLPMVRRVKSGLRRRTV"
BASE COUNT          428 a          635 c          584 g          473 t
ORIGIN      
        1 ctccatgtca cgcgttggtg aaatattggt cattgatcca gccaccatca cccgtagcgt
       61 gcggttgctg caaaacgacg gcctgctgga aatcgcccca cggggcttgc ggcgtcaacg
      121 cttactcact ttgacgtcaa aagcggaaaa agttctcgcc attgccgtcc cgctgtggcg
      181 ggaggcccaa gcccgcttta tcgccagtct gggcggcaaa tggaaagaat ttcaggacga
      241 actggagcat gcggcagagg tagccgtctc gctggaaatc gctgatacag cggcagatac
      301 ggctgcgcag cgcaactcgc cgcgccagaa gaatcgagct tagactgtgg cctcacgcgc
      361 gttggcgaag agttgtcgac cccacttcag ccggatcgtc ttgtaaaaag tttgtcactt
      421 gagtccatcc ggaccggtgt tcgattgtgt cagaaccggt tcaggcggtt gcgctctgcg
      481 cgcgaaacgc cgctgcgttt tcggcgagga attgctccat ggtgatcggc ggccggccaa
      541 tcacccgctg caacacgtcg tctacttccc cgccctttcc attcacgtaa tactgctgca
      601 aatccagcag tgccgttacc tgccactcgg gcatgcccaa atcgagcatg gatttgcgct
      661 gtgcctccat cggaatgtct acgtatttca cggggcgtcc ggagtgtttg gcaatctcct
      721 cggcgacttc attcgcggtg agtgcctccg gtccgttcag ttcgtaaatt ttccccgaat
      781 gctctctgcc ggacaacgtt cttgcgacgg ctccagccac gtcacgcaca tcgatataac
      841 tcgtccgggc gtttcccaag gaactgtaga aaactccctg cgcacggatg ctcggcgcgt
      901 agtaggtcac cgtattttgg tgaaaactgt tgggtcgcag gatggtccac gagagttttg
      961 ttccgcgcag cttgtcttcc actttgcggt gccaactggg gaacgatttg tcgtagtcgc
     1021 cagcgccaag cgcggagttc agcacgacat gcgtgactcc cgcttccacg caggcatcga
     1081 tcatattgct ttccagttcg accagttcgg gaaccggccc gcacaccagg tacacgctac
     1141 tgacaccgcg cagtgctgat cctagcgctt cttttttcgc gaaatctgca attaccgttt
     1201 cggtgcccgg cggcgctttc gtcgcctcca cggccgaacg gtacatcgcg cggtgcttcg
     1261 caccgctttt cgctaccgcc tgcagcactt ctcgtcccgc cgttccgctt gctcccgtaa
     1321 tcaaaatcat gccgtgctcc ttgccagccc ctggctttcc aaaacgacgc ggtttgccaa
     1381 cgtcgttgcc gctagaaatg ctataacggt gccattgcgg tgagaagtac gcacaatttt
     1441 gtaagctgag gtcaactcaa tgaagggcca tgggaacggc aatcagaaga acgactttgg
     1501 ctgtccggtg caggggacaa tcaacgtgct cagcgggaaa tggaaaggcc tcgcggtgtg
     1561 gcatctgggc tttggagcga agcgctttgc cgagctccgt gatttgcttc ctggagtcag
     1621 cgaaaaagtg ctgacttctc agcttcgcca gctcgaatcc gacggagttg ttcggcgcag
     1681 agtcaaaccg acttcgccgc cgcaagtgac gtattcgttg accaaagccg gaggcgaact
     1741 catcccaatg atgagcgact tgtgtcattg ggccacgaag cacctcggca tacccccaat
     1801 gcttccgatg gtgcggcgtg tgaagtccgg cttgcgacga cgtacggttt agctcgcgct
     1861 acgtcgccgc ttggcggagc cagaacccgg ccgcagttgc gcagtttatt gcgccgaacg
     1921 cttcgctgac gattaatggt ggcgcgagtc acctgcacct cccagcaccc gacttttcgg
     1981 gaccgactga tcccccaaat ctgcgcggcg ctgccgaact tgatccgaaa aaccgtcacg
     2041 cggatgagaa aatccgccaa caactgcctg aactcggaaa gctgggtatt tcaaaatttg
     2101 tcaaaccagg tttctacgaa
//
LOCUS       DASTPM010000684         4202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_831797, whole genome shotgun sequence.
ACCESSION   DASTPM010000684 DASTPM010000000
VERSION     DASTPM010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831797"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFR42_12065"
     CDS             complement(<1..504)
                     /locus_tag="VFR42_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369943.1"
                     /translation="MAISLGKVAVAFLLLVLVSLSSAPLFSQTPEPIRLHPKNPHYFL
                     FRGKPTVLITSGEHYGSVLNADFDYKKYLATLAADGMNFTRIFGGSYVEVPATSFGIK
                     RNTLAPQPGRFLAPWMRSEVGGYAGGGNKFDLSRWNPEYFRRYQEFLAEAAKAGIVVE
                     ISLFSSTY"
     gene            complement(582..890)
                     /locus_tag="VFR42_12070"
     CDS             complement(582..890)
                     /locus_tag="VFR42_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369944.1"
                     /translation="MRHRRGDVSRGACRCRDRATLVIGEVRELSARFGGRISSRLVAM
                     HGYAADAVHTWFHGGTVYSTTPRNWGSGRRPYRRPAAIRPAIGLGQAGGLWGPKTVVT
                     "
     gene            1106..1453
                     /locus_tag="VFR42_12075"
     CDS             1106..1453
                     /locus_tag="VFR42_12075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0369945.1"
                     /translation="MALKLTQREVDGVTVVALDGRIVLGEESNALREKVKSLLAEGKK
                     KIVLNMDNITFIDSAGLGTLVASHHTAKKEGASLRLCHLGSKFQEVLQITKLLTVFEV
                     YDTEAAAVASFSQ"
     gene            complement(1546..2892)
                     /locus_tag="VFR42_12080"
     CDS             complement(1546..2892)
                     /locus_tag="VFR42_12080"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEU0369946.1"
                     /translation="MAQVFPFGALRFNPQVAPSELVLTQPYDKISPQRQAEYYAMHAH
                     NLIAVEKGRTHENDDAHNNVYTRAAAKVEEWIRDSVLLQDPAPAFYAYSQEFTVPGSN
                     ERRTRSGFIGAGQLEDYSAGVVFRHEHTLSGPKADRLELLRHTRLHTGQLFMLYSDPQ
                     RRVDTVLAGAEKSAAPATELTDEYGVIHRLWPITEPQRIHAIQQAMADHKLVIADGHH
                     RYETALNYRNERRAQAGASAVPAASYERAMMTFVNTRSEGLVILATHRVVANVHDFSW
                     SAVRRYLEPWFSAEVFPFANDSEQVQARDKFLARLKDTAAQRSIGAYPVANGQRAFYL
                     FHLRPGADLGQLLPNVPALQRELDVVLLHDGILEPALGITLQTATKEINLTYEREAAA
                     AIAAVDSGRAQVSFLLNPVDVEKVVEIATANEVMPQKSTDFYPKLLSGITNYRLDL"
     gene            3112..3804
                     /locus_tag="VFR42_12085"
     CDS             3112..3804
                     /locus_tag="VFR42_12085"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEU0369947.1"
                     /translation="MNEKLRGRIKAVVFDLDGTLIDSKQDLVLSVNATLRAIGREELP
                     TELVASYVGSGAPVLISRALGGPADPDLLQRALKFFLGYYEEHKLDYTREYPGVRQAL
                     EELNGMPMAVLTNKPVNISVRILEGLGLAQFFKAIYGGNSFATKKPDPLGANTVLGDL
                     KVGASEAAMVGDSEVDVQTARNAGMISAIVNFGFGMHDREKYPADVYLDRMEELVPLV
                     MDGTGGSATLRK"
     gene            3858..>4202
                     /locus_tag="VFR42_12090"
     CDS             3858..>4202
                     /locus_tag="VFR42_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369948.1"
                     /translation="MLIGLFPELDAPGGVQRAGRHLASVLTEFAASRGMECRILTLND
                     GPELKRLGVAGREIVFTGSERAKGRFLATAMRAARRRTGGGKSKTKIVVAGHPNLAPV
                     VRAMHFLSPRLKS"
BASE COUNT          893 a         1098 c         1385 g          826 t
ORIGIN      
        1 atacgtcgag gagaacaacg aaatttccac cacgattccg gccttcgcgg cttcggcaag
       61 aaactcttgg tagcgccgga aatattccgg gttccacctg ctcagatcga acttgttgcc
      121 accgcccgcg tagccgccaa cctcgctgcg catccacggc gcaagaaatc ttcccggctg
      181 cggggccagc gtgttgcgct tgatgccaaa agaggtcgcc ggaacttcca catagctgcc
      241 cccgaaaatg cgcgtgaaat tcatcccgtc cgccgctagc gtcgccagat attttttgta
      301 gtcaaaatcc gcgttcaaca ccgagccgta atgctccccg ctggtgatca gcacggtggg
      361 cttgccgcga aacagaaagt aatgcggatt tttcgggtgc aaacgaatcg gttcgggagt
      421 ctgtgaaaaa agcggcgcgc tgctgagaga aacaagcacc aggaggagaa acgcgacagc
      481 aaccttaccg agggaaatcg ccactgcagc agctccttgg ccggcaaagc cgtagcatga
      541 aatgccggcg aatcaatcgc gcggcgcgca aaatggccgc gttaggttac caccgttttc
      601 gggccccata gacctccggc ctggccaaga ccgatagcgg ggcggatcgc ggcgggacgg
      661 cgatacggcc tccgcccgct accccaattt cgcggggttg tgctataaac ggtgccgccg
      721 tggaaccagg tgtgtactgc atcggcagcg taaccgtgca tcgcgacgag tcgcgagctg
      781 atcctcccgc cgaagcgagc ggaaagttcg cgcacttcgc cgataacgag cgtggcgcga
      841 tcgcggcaac ggcaagcgcc gcggctcacg tccccgcgac ggtgccgcaa gaggaccctt
      901 cgcgattctg tccggtgtgc tcgcagcggc tggaatcgcg gcgctgtaaa ctaatttgca
      961 acgtttgcgg ctactatatg tcctgcgcgg actattactg accggatttc aggctatttt
     1021 acaaaccctg aggtcacggg atatgctttg ggactccgga attggttgcc gacggacccg
     1081 cttaacgaat gaggaggaaa agaacgtggc attgaaattg acgcaacgcg aagtggacgg
     1141 cgtaacggtg gtcgcgctgg acgggcggat cgtgcttggc gaagagagca acgcgctccg
     1201 cgaaaaagta aaaagcctgc tggccgaggg caagaaaaag attgtgctga acatggacaa
     1261 catcacgttc attgacagcg cgggcttggg cacgctggtg gcttcgcatc acaccgccaa
     1321 aaaagaaggc gcgtcgctgc gcctgtgcca cctgggcagc aagttccagg aagtcctgca
     1381 aatcaccaag ctgctgacgg tgttcgaggt gtatgacacc gaagcggcgg cggtggccag
     1441 cttctcccag taaattcttt ttgttttgcg gcgagttttc gcagaagagg cgcgccatcg
     1501 cgcctcttct gtttttatgg ccgcgttttt gtggccgcca aaatctcata agtcgagacg
     1561 ataattcgta attccactga gcaacttggg atagaagtcg gtggactttt gtggcatcac
     1621 ttcgttggcc gtggcaatct cgaccacctt ctccacgtcg acaggattca aaaggaaaga
     1681 aacttgcgcg cggccagaat ccacggcggc gatcgcggcg gcggcttcgc gttcgtaggt
     1741 gagattgatt tccttggtgg cggtctgcag cgtgatgccg agagcgggct ccagaattcc
     1801 gtcgtgcagc agcaccacgt cgagctcccg ctggagggct ggcacgttcg gaagcaattg
     1861 cccgagatct gcgccggggc gtaagtggaa gaggtagaag gcacgctgcc cattcgctac
     1921 cggatatgcg ccgatcgagc gctgtgcggc ggtgtctttg agacgggcca ggaatttgtc
     1981 gcgcgcctgt acttgctcgg aatcgttggc gaagggaaat acttcggcgg aaaaccacgg
     2041 ctccagatag cggcgcacgg cggaccagga aaagtcgtgt acgttggcaa cgacgcgatg
     2101 cgtggcgaga atgaccagac cttcgctgcg cgtgttgacg aaagtcatca tcgcgcgttc
     2161 gtagctggcg gcgggcacgg ccgaagcgcc ggcttgcgcg cggcgttcgt tgcgatagtt
     2221 gagagcagtt tcgtagcgat ggtggccatc ggcgatgacc agcttatgat cggccatggc
     2281 ctgctgaatt gcgtggatgc gctggggttc agtgatgggc cacaagcgat gaatcacgcc
     2341 atattcgtcg gtgagttcgg tcgcaggcgc agcggatttc tccgcgccgg cgagcactgt
     2401 gtccacgcgc ctttgcggat cggaatacag catgaagagc tggccggtgt gcagacgtgt
     2461 gtggcgcagc aattcgaggc gatcggcttt gggacccgag agcgtgtgct cgtggcgaaa
     2521 gacgacgcca gcggaatagt cttccagctg gccggctccg ataaaaccgc tgcgcgtacg
     2581 gcgttcgttc gagccgggaa cggtgaattc ctgcgagtac gcgtagaatg ccggcgcggg
     2641 gtcctgcagc aggacggaat cgcgaatcca ctcttcgact tttgcggcgg cgcgagtgta
     2701 gacattgttg tgcgcgtcgt cgttttcgtg ggtgcggcct ttttccaccg cgatcaggtt
     2761 gtgcgcgtgc atggcgtagt attcggcctg gcgctgcggg gaaatcttgt cgtagggctg
     2821 ggtgaggacg agctcggacg gggcgacttg cgggttgaag cgaagtgcgc caaacgggaa
     2881 tacctgggcc atgagttctc cggtgttttc tcagagagag cggggaaaag gtggaatgcc
     2941 actcctccgg ggctaaactc cgcctcatcg ccaagccact gatgtcgcag ctgaagccgc
     3001 gaccccctga agagaatctc tcctcctgat ctctccggca caagggcgga aacaacatag
     3061 tttaatcgaa gcgaagcgga gaatgcgacg cgcgtattaa aataaaaaga tatgaacgag
     3121 aaattgcgcg ggcgaatcaa ggcggtggtg tttgacctgg atgggacact gattgattcg
     3181 aaacaggacc tggtgctttc ggtgaatgcg acgctgcgag cgatcgggcg ggaagagttg
     3241 ccgacggagc tggtggcgag ctacgtaggt tcgggtgcgc cggtgctgat cagccgggcg
     3301 ctgggcggac cagctgatcc ggacttgctg cagcgggcgc tgaagttttt tctggggtat
     3361 tacgaggaac acaaactgga ctacacgcgg gagtacccgg gagtgcgcca ggcgctggag
     3421 gaactaaacg gaatgccgat ggcggtgctg accaacaagc cggtgaacat cagcgtgcgg
     3481 attctggaag ggctggggtt ggcgcaattt ttcaaagcga tttatggcgg aaacagtttc
     3541 gcgacaaaga aacccgatcc gctgggagcc aacacggttc ttggcgattt gaaagtcggg
     3601 gcaagcgaag ccgcgatggt aggcgactcc gaagtggacg tgcagacggc gcgcaatgcg
     3661 ggaatgattt cggccatcgt taattttgga tttggaatgc acgatcggga gaagtatccg
     3721 gcagatgtgt atctggatcg gatggaagag ttggtgccgc tggtgatgga tggaaccggc
     3781 ggaagtgcca ctttgcgaaa ataggagact cacggtgaag gcttgcgaat gagtgcggca
     3841 caaggcagac tcggcgcgtg ctgattggac tatttccaga actggacgcg ccgggcggag
     3901 tgcagcgtgc cgggcggcat ctggcctcgg tgctaaccga gtttgccgca agccgcggta
     3961 tggaatgccg tattctgact ttaaatgatg ggccggagtt gaagaggctg ggcgttgcgg
     4021 gacgcgaaat tgtcttcacg ggaagcgagc gagccaaggg caggttcctt gcgacggcga
     4081 tgcgcgcggc acggagacga actggcggcg gaaaatcgaa gacgaaaata gtcgtcgcag
     4141 ggcatcctaa tctcgcgccc gtcgtgcgcg ccatgcattt tctctcgcct cggctgaagt
     4201 cg
//
LOCUS       DASTPM010000685         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_947328, whole genome shotgun sequence.
ACCESSION   DASTPM010000685 DASTPM010000000
VERSION     DASTPM010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947328"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFR42_12095"
     CDS             complement(<1..338)
                     /locus_tag="VFR42_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369949.1"
                     /translation="MKIILSSMLALAIAGSPPLTQEPDEKSKPKPEKPKQEPAPPPKQ
                     PKQDERQQQDEKDRQKQQKEQAKRENNRSPQEQNAPRDNHKQAGAQSKGQRIPPEKFR
                     TSFGREHHFRV"
     gene            complement(610..2280)
                     /locus_tag="VFR42_12100"
     CDS             complement(610..2280)
                     /locus_tag="VFR42_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family serine peptidase"
                     /protein_id="HEU0369950.1"
                     /translation="MNLSMNQSGGIGAVAIVRATCVALSCAMLLFSAGTKAQEQSMPT
                     RHIHQEMAGGQAKILGHLPETQQLKLNIALPLRNEAELDDLLRRLYDPKSGLYHQFLS
                     VEEFTARFGPTEQDYTEAARFAEEKGLKVTGTYENRMVLNVTGSVGNIEKALHVKMNS
                     YKHPTDNRAFYSADREPAPELGIKLWHITGLDNFSIPQPAGLKRDAQAPPHTTGSGPS
                     GYFLGSDFRAAYYGGTALTGAGQSVGLLEFAGYNQADVTKYFSTVGQPLTVRVVGVST
                     DGSSLSCSGGCDDTEQVLDIEVAISMAPGMNQVRVYVSDTSDVSMFNKMASENLAKSL
                     SCSWGWSPADPSSDDPIFKEFAAQGQNLFVASGDSGAYRKKSRYVYPADDAFVTSVGG
                     TDLVTASPGGPWQSETAWNGSGGGISPNGIAIPTYQKAGGVITTGNKGSTTLRNAPDV
                     AAEGNTDNFICYDGKCAGGWGGTSFAAPRWAGYLALVNQQSVSHGHATAGFLNPTIYS
                     IGLGSSYGADFHDITSGSNGTYSTIKSYDLVTGWGSPIGAALINALAP"
BASE COUNT          449 a          816 c          644 g          621 t
ORIGIN      
        1 acccgaaaat gatgctcgcg tccaaagctg gtgcgaaact tttccggcgg aatgcgctgg
       61 cctttgctct gtgccccggc ttgcttgtga ttgtcacgcg gcgcgttttg ttcctgtggc
      121 gatcggttgt tttctcgttt cgcttgttct ttctgctgtt tttgccgatc cttttcgtcc
      181 tgctgctgcc gttcgtcctg cttcggctgt ttgggcggag gcgccggctc ttgtttcggt
      241 ttttccggct ttggtttcga tttctcgtcc ggttcctgcg tcaaaggagg cgagcccgca
      301 atggccagag ccagcatgct gctcagaatg atcttcatcg gttactccgt ctcgttgcga
      361 attttttggc cgaggctgca acagcttgct accttggcca actccaggcc ggtaagcgaa
      421 gtccgcttca cttaagcaaa cactgatctg gcgctgcagc cattactgtt ttcactttaa
      481 tccgaaccga agtttgtctt aggcaggaat aggtagacgc actgagatga cgcgttcaaa
      541 acaaaaaatg cacgtggttg ttacaccgcg cgcactcttc gttcaacttg cttcgactgg
      601 cttgcttcgt cagggcgcca gcgcgttgat cagcgccgcg ccgattggac tgccccagcc
      661 cgtcacgagg tcatagctct tgatcgttga atacgtccca ttgctcccgc tcgtgatgtc
      721 gtgaaagtcg gctccatagc tcgaccccaa accgatcgaa taaattgtcg ggttcaggaa
      781 gcccgccgtc gcatgtccgt gcgagacgga ctgctgattc accaacgcca gatagcccgc
      841 ccagcgcggc gccgcaaagc tcgttccgcc ccacccgccg gcgcattttc cgtcgtagca
      901 aatgaaatta tcggtgttgc cttctgccgc cacgtccgga gcattgcgca acgtcgtgga
      961 acccttgttc cccgtcgtga tcacaccgcc ggccttttgg taagtgggaa tggcaattcc
     1021 attgggcgaa attcctcctc cgctaccatt ccacgccgtc tccgattgcc acggcccgcc
     1081 cggactcgcc gtcaccaggt ccgttccgcc caccgaggtc acaaaagcat catccgctgg
     1141 atacacatag cgcgactttt tccggtacgc gccgctgtct cccgacgcca cgaacaggtt
     1201 ttgcccctgc gcggcaaact ccttgaaaat cggatcgtcg ctgcttgggt ccgccgggct
     1261 ccagccccac gaacagctga gcgacttcgc cagattctcc gaagccatct tgttgaacat
     1321 gctgacatcg ctggtgtcgg acacatacac gcgaacctgg ttcattcccg gcgccatcga
     1381 aattgccact tcgatgtcca atacctgctc cgtgtcgtcg cagccccctg agcagctcag
     1441 gctggagccg tccgtcgaaa ctcccaccac tctcaccgtc agcggctgcc ccaccgtcga
     1501 aaaatatttc gtcacgtccg cctggttgta gcctgcgaat tccagcaggc ccaccgactg
     1561 ccccgcgccc gtcagcgccg ttccaccgta ataggccgct cgaaaatcgc tgcccaaaaa
     1621 gtagccgctc ggcccggagc ccgttgtatg cggcggcgcc tgcgcgtcgc gtttcaatcc
     1681 cgccggctgg ggaatcgaaa aattatccag ccccgtgata tgccacagct ttatgcccag
     1741 ctccggcgcc ggttcgcggt ccgccgaata aaacgcccgg ttatccgtgg gatgtttata
     1801 tgaattcatt tttacgtgca gcgccttttc gatgtttccc acgcttccgg tgacgttcaa
     1861 caccatgcgg ttttcatacg tccccgtcac tttgagtcct ttctcttccg cgaaccgagc
     1921 cgcttccgtg tagtcctgtt ccgtcgggcc aaaccgcgcc gtaaactctt ccacgctcaa
     1981 aaactggtga tacagcccgc ttttcgggtc gtacagcctc cgcaacagat cgtccagctc
     2041 cgcttcgttg cgcaacggca gcgcaatgtt cagcttcaat tgctgtgtct ccggcagatg
     2101 tcccagtatt tttgcttgcc cgcccgccat ctcttggtgg atgtggcgcg tgggcatcga
     2161 ctgctcttgc gctttcgttc ccgctgagaa cagcaacatg gcgcaagaca atgccacgca
     2221 tgtcgctcgc acaatggcga ccgcacctat tccgcccgat tgattcatcg acaggttcat
     2281 cgattggctc cttggtttct tgcgtgctgg agtttctcgt gtgggcaggg gtacctatca
     2341 gcgatacaca cgatcgcttg gttcgacact cgcgtgaaga ccacttcgac aactacgctc
     2401 caggtctcga gcatcgtacc cttctcgccc gcgccagaca acggatttgc tctgcacgga
     2461 tttgctttgc agtaatggcc agcttccctt agcccgagca atagggctgt gattgacact
     2521 gctgaccatg
//
LOCUS       DASTPM010000686         4554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_803513, whole genome shotgun sequence.
ACCESSION   DASTPM010000686 DASTPM010000000
VERSION     DASTPM010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803513"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFR42_12105"
     CDS             <1..222
                     /locus_tag="VFR42_12105"
                     /inference="COORDINATES: protein motif:HMM:NF017330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase domain-containing protein"
                     /protein_id="HEU0369951.1"
                     /translation="FDAYSGKTEKRTVQTGHIATVYAELHKSYGWYDLKVKAESDAGF
                     SRQLAGHVETGRASVTDPAIASAAVTQAV"
     gene            complement(308..739)
                     /locus_tag="VFR42_12110"
     CDS             complement(308..739)
                     /locus_tag="VFR42_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369952.1"
                     /translation="MKRNYFRMLVVSLFVASIGVAARAGEPDRLLVKIPYDFVVSGKT
                     LPAGAYHFSRVSDRDLHQIAISSVDTGSTVLTLSAQVQDTSRLQPKITLQHSGDQNFL
                     SKIQTANHVFTIAVPKSALAEAAAKAQGGSYLSGTAESSKR"
     gene            complement(1004..2038)
                     /locus_tag="VFR42_12115"
     CDS             complement(1004..2038)
                     /locus_tag="VFR42_12115"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU0369953.1"
                     /translation="MSTTTLPVLTKKDFQTDQEIRWCPGCGDYAILAAVQAVFPELGI
                     KRENFVVVSGIGCSSRFPYYMNTFGFHTIHGRAPAVATGLKAIRPDLEVWVATGDGDA
                     LSIGGNHTIHMLRRNVGLKVLLFNNRIYGLTKGQYSPTSEIGKKAKSTPYGSLDRPFN
                     PISLAIGAEATFVARSVDVFQAHLKDTLKQAAAHHGSAFVEILQNCNIFNDGAWTGIT
                     EKDARSEHVIQLEHGKPLVFGKNRDKGIRRKSDGDIEVVQLGNDITESDLVVHDAHHP
                     RPSYAFLLSHMEHREGFPTPIGVLRAWDDLPRYEDVINQQIRDVQAKRGTGDLAKLLR
                     GGDTWRVEAR"
     gene            complement(2173..4029)
                     /locus_tag="VFR42_12120"
     CDS             complement(2173..4029)
                     /locus_tag="VFR42_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU0369954.1"
                     /translation="MATTTDIPLPKHKREVIDRAVIRFAGDSGDGMQVTGSQFTNTVA
                     LYGNDIATFPDFPAEIRAPAGTLPGVSGYQLHFSSNEIYTPGDAVDVLIAMNPAALKM
                     NLADLKANGILIVNSDAFAETDLRKAQLTVNPLEDHSLDKYRLFSVELERLTKVALEH
                     LGLDAKSVSRCKNFFALGMCYWLYNRSMAGTVRYIEEKFAKKPLLVEANKLAMKAGYS
                     YCEATEAFQISYEIPPAQLEPGLYRNMSGNQALALGFITASQKSGLKLFQGSYPITPA
                     SDILHELSQYKDFGVITFQAEDEIAAITSAIGAAYAGALAITTTSGPGMALKTEAMGL
                     AVAVEIPLVVCDIQRGGPSTGLPTKTEQADLLQALFGRNSEAPIPVIAPATPSDCFWA
                     ALEASRIAVKYMVPVIILSDGYLANGAEPWKIPDLDAIPDFKVNFASDPVDFQPYRRN
                     PDTLARPWAVPGTPGLEHRIGGLEKQDVTGNINYEPLNHENMVRIRAAKVEAIAQDIP
                     DAVPAGDPDGELLIVAWGSTHGAITAAVNAQRELGRRIGHVHLRHLNPLPSNLGDVLK
                     RYKQVLVPELNMGQLLWLLRAKFLVDASGLNKIQGRPFKQSELEQKISELFS"
     gene            complement(4345..>4554)
                     /locus_tag="VFR42_12125"
     CDS             complement(4345..>4554)
                     /locus_tag="VFR42_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369955.1"
                     /translation="LLSMRDGWGIPAPLAPETAALVREMRWIFLISVVMPLAILVVLC
                     IYRRPFYSAAEIADKRAFSSGRAAS"
BASE COUNT          965 a         1086 c         1562 g          941 t
ORIGIN      
        1 ttcgatgcgt atagcgggaa gacggagaaa cgcacggtgc aaacgggtca tatagcgacc
       61 gtttacgccg agctgcacaa atcgtatggc tggtatgacc tgaaagtgaa ggctgagagc
      121 gatgcaggat tttcgcgtca actcgccgga cacgtcgaga cggggcgagc tagcgtcacg
      181 gacccagcga ttgcatcagc ggccgttaca caagcggtct gagccggcga agcgcctttc
      241 cggcaaggca caatcggcct ccaagaacat cttggaggcc gacttttcat gccgacgcga
      301 tgcgaaatta gcgcttgctg gattcggcgg tgccgctcag gtacgaaccg ccttgagctt
      361 tggcagcggc ctcggcaaga gcggactttg gcaccgcaat ggtgaaaaca tggtttgcgg
      421 tctggatctt tgataggaag ttctgatcgc ccgaatgttg aagagtgatt ttgggttgca
      481 agcgcgaagt gtcctggacc tgagcggaaa gggtcagaac ggtggagccg gtgtcgacac
      541 tgctgatggc gatctgatgc aagtcgcgat cgctgacgcg gctgaagtgg taggcgccgg
      601 cgggaagagt cttgccgctg acgacgaagt cgtaaggaat cttgaccaga agccgatcgg
      661 gctcgcctgc tcgggctgcg actccgatcg aggcaacaaa tagagacacc acgagcatgc
      721 ggaaatagtt ccgtttcatt ccttttctcc ctttttagtt tggagtgggt agcgttatgc
      781 gccttttgcg gccggagtga agacgaccgc ggtcagcatc gttttgcgaa tgcagcccca
      841 aacgtgtatg tccattggac acgaacagag gaagcggagt tagaccgaga aagtaaagct
      901 tgtgtaacga atttggagga agaaaagata gacagaagtt agatagagcg ttggccctga
      961 tcgaaaatga aaacttgtgc gggttggcgc agtggctggc ttctcagcgg gcttcgacac
     1021 gccaggtgtc gccgccgcgg agaagcttgg cgaggtcgcc ggtgccgcgc ttggcttgca
     1081 cgtcgcgtat ctgctgattg atgacgtctt cgtagcgcgg gagatcgtcc caggcgcgca
     1141 gaacgccgat cggagtaggg aaaccttcgc gatgctccat gtgggagagc aggaaggcgt
     1201 aagaaggacg cgggtgatgg gcatcgtgga cgaccaggtc cgattcggtg atgtcgttgc
     1261 cgagttgcac gacttcgatg tcgccgtcgg atttacggcg gatgcctttg tcgcggttct
     1321 tgccgaaaac caagggcttg ccatgttcga gttggatgac atgttcgctg cgcgcgtctt
     1381 tttcggtaat gccggtccac gcgccatcat tgaagatgtt gcagttctgc agaatttcga
     1441 caaaggcgga gccgtgatgc gcggcagctt gcttcagggt gtctttcagg tgggcttgga
     1501 agacatcgac ggagcgagcg acgaaggtag cttcggcgcc gatggcaaga gaaatgggat
     1561 tgaacggtcg atcgagcgag ccgtacggag tggactttgc ctttttaccg atttcagagg
     1621 tcggcgaata ttggcctttg gtgaggccgt agattcgatt gttaaagaga agaaccttga
     1681 ggccgacgtt gcggcgcagc atgtggatgg tgtgattgcc gccgatggag agagcgtcgc
     1741 cgtctcctgt agcgacccag acttcgaggt cggggcgaat ggctttgagg ccggtggcga
     1801 cggcgggcgc gcggccatga atggtatgga aaccgaacgt gttcatgtaa tacggaaagc
     1861 gcgaagagca gccgatgcca gacacgacca cgaaattttc acgcttgatg ccgagttcgg
     1921 ggaagacggc ctggacggcg gcgaggatgg cgtagtcgcc gcagccgggg caccagcgga
     1981 tttcctggtc ggtctggaag tcttttttgg tgaggacggg gagagtagtg gtgctcatta
     2041 acaaacgctg cctttctgca atctcggtag cggatcaatc aagcagcaga ttcctcggct
     2101 gcgcctcgct ccgctccgaa tgacaaaccg tctagtcaaa acacgtcaaa agcacgggcc
     2161 taagccccgc cgctacgaga agagctcaga gattttttgt tcgagctcgg attgcttgaa
     2221 cgggcggcct tgaattttat tgaggccgct agcgtccacc aaaaattttg cacgcaaaag
     2281 ccagaggagc tggcccatat tcagttcggg aacgagcact tgcttgtagc gcttgagaac
     2341 gtcgccgaga tttgacggca atgggttcaa gtggcgcaga tggacgtggc cgatgcggcg
     2401 gccgagttcg cgctgcgcgt tgacggcagc ggtgatggcg ccgtgcgtgg agccccaggc
     2461 gacgatgagt aattcgccgt cgggatcacc cgcgggaacg gcgtctggaa tgtcttgcgc
     2521 gatggcctcc acttttgcgg cgcggatgcg aaccatgttt tcgtgattca ggggttcgta
     2581 gttgatgttg ccagtgacgt cctgtttttc gaggccgccg atgcggtgct cgaggccggg
     2641 cgtgccgggt accgcccagg gacgcgcgag agtatcggga ttgcggcgat agggttggaa
     2701 gtcgaccgga tcggaggcga aattcacctt gaagtccggg atggcatcga ggtcgggaat
     2761 tttccagggt tcggcgccgt tggcgaggta gccgtcggaa agaatgatca cggggaccat
     2821 atatttgacg gcgatgcgag aggcttcgag ggcggcccaa aagcaatcgc tgggagtggc
     2881 gggagcgatg acgggaatcg gggcttcgga gttgcggccg aagagagctt gcaataggtc
     2941 cgcttgttcg gttttcgtgg gcaagccggt ggaaggtccg ccgcgctgga tatcgcacac
     3001 gaccagagga atctccacgg ctacggcaag acccatggct tccgttttga gcgccatgcc
     3061 gggaccagaa gtggtggtga tggcgagcgc gccagcgtag gccgcgccta ttgcggaagt
     3121 aatggcagcg atttcgtctt ccgcctggaa ggtgatgaca ccgaaatcct tgtactggga
     3181 aagttcgtga agaatgtcgg aagcgggcgt gatgggatag ctgccctgga agagtttcag
     3241 gcccgatttt tgcgaggcgg taatgaaccc gagagcgagg gcctggttgc cgctcatgtt
     3301 gcggtagaga ccgggctcga gttgcgccgg cggaatttcg tagctgatct ggaacgcttc
     3361 ggtggcttcg cagtaggaat agccggcttt catggccagc ttgttggctt cgaccaggag
     3421 cggcttcttg gcgaattttt cttcgatgta gcggactgtg cctgccatcg agcggttgta
     3481 aagccagtag cacatgccca gagcgaagaa atttttgcac cggctgaccg acttggcgtc
     3541 gaggccgagg tgctcgagcg cgactttggt gagccgttca agttcgaccg agaagaggcg
     3601 gtacttatcg agggaatgat cttcgagggg gtttacggtg agttgcgctt tgcgcagatc
     3661 ggtttcggca aatgcgtcgg aattgacgat gaggatgccg ttggccttga ggtcggccaa
     3721 attcattttg agggcagcgg gattcatggc gatcaggacg tcgaccgcgt cgccgggcgt
     3781 gtagatttcg ttcgaagaga agtgaagctg gtaaccgctg acgccgggga gagtaccggc
     3841 aggggcgcga atttcggcgg ggaagtccgg gaaggtggcg atatcgttgc cgtagagggc
     3901 taccgtattg gtgaactggc tgccggtgac ctgcatgccg tcgccggagt ctccagcgaa
     3961 gcgaatgacg gcgcggtcga tgacctcgcg cttatgcttt ggcagcggaa tatcggtggt
     4021 agtggccatg cgttgttgcg cttctcccga aaggcatcat gcggccttcc tcccctgtga
     4081 atatctgcgg ggcgagccgt ctggctggaa cagcgtgcag gttggagctg aagcgagccg
     4141 ccaggtgcct ggagaaaaat gatagcacag cgcggcgaac gaggcgcgaa agtgcgaggc
     4201 tagtcaacag tgaaaattcg aattggaaga attgaagaaa aggcaagaaa ggctggctgg
     4261 cggttgataa ttagcgactt gcagcgagcc ttcgtgggtc cgattcgacc ggcccaggaa
     4321 ggcaagagaa tgggaaggtc ccgttcaaga agcggcccgc ccggaagaga aggctctttt
     4381 gtcggcaatc tcggcggcgg aataaaaggg acgacggtag atgcagagga caacaaggat
     4441 ggccagaggc atgactacac tgatcagaaa aatccatcgc atttcgcgca ccagggcggc
     4501 ggtttccggt gcgagtggtg caggaatgcc ccagccatcg cgcatggaga gcaa
//
LOCUS       DASTPM010000687         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1149039, whole genome shotgun sequence.
ACCESSION   DASTPM010000687 DASTPM010000000
VERSION     DASTPM010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149039"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFR42_12130"
     CDS             <1..493
                     /locus_tag="VFR42_12130"
                     /inference="COORDINATES: protein motif:HMM:NF020680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HEU0369956.1"
                     /translation="APLAGSEVEAILKDKSDLSPAAIRTAAQLAEGSPGYALEMDVEK
                     ESQRRREALKILDRASRGEEFGQIFAQTSALAKDRDISFEDQLSVFYSLLSDLLELSS
                     GIHTPQIRNPPLRKELEVTARHFSVDSIQRALSGLDQLAAGARRNLNKQLGLEAFAAD
                     LSS"
     gene            673..1008
                     /locus_tag="VFR42_12135"
     CDS             673..1008
                     /locus_tag="VFR42_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEU0369957.1"
                     /translation="MFRPSFTEAKEPIPVRPNNHQPHQSHNGPAPTKKPTPPEMTHAE
                     NFYWIKQMQSRTPMVIVMDNGESLHGIVEWYDRDCIKLTRHGSANLMIFKKNIKYVYK
                     EGEEGGSEE"
     gene            complement(1011..1514)
                     /locus_tag="VFR42_12140"
     CDS             complement(1011..1514)
                     /locus_tag="VFR42_12140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0369958.1"
                     /translation="MKFTREHLEYARSRVKLSKFSALMGFEVAKLYPGGAILQVVTED
                     HHRQVHNVMHGGVIAALADTAAAIAAYTVIPRGAEVVTIELKINYLLPVAEGKLKAEG
                     KVLRAGRNFIVVECDVTKQDGALAAKALMTFGAAPGHVLTGKPATEAGPKKSRVAGPT
                     KRKKKNG"
     gene            1713..2852
                     /gene="bshA"
                     /locus_tag="VFR42_12145"
     CDS             1713..2852
                     /gene="bshA"
                     /locus_tag="VFR42_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEU0369959.1"
                     /translation="MRIGITCYPTYGGSGVVATELGQELAARGHDVHFISYAPPIRMN
                     PNDPHIFFHEVEVASYPLFDHPPYALALATKMLEVFESEALDLLHVHYAIPHSVSAML
                     ARSMAAPRRLPFITTLHGTDITLVGSNRSYLPITKFSIEQSDGVTAISRYLLNQTMKE
                     FDIKRPIEVIPNFVNCDLYTRQPNLAQRGKWAPAGEPILMHLSNFRPVKRVTDCVEIF
                     AQVRAKMPAKLVLIGDGPERGAAEYLVRKKKLQKDVIFLGKQDQVYRLLSDADLFLLP
                     SQLESFGLAALEAMACEVPVIATNVGGVPEVIEHNVDGYLVEPGDVAAASKYAIELLS
                     RADRGREMGELARVNAKKKFCANDVIPSYERYYQRVLSETRAASA"
     gene            complement(2913..>4080)
                     /locus_tag="VFR42_12150"
     CDS             complement(2913..>4080)
                     /locus_tag="VFR42_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369960.1"
                     /translation="MLFVALPFIWLGLAMTVKRLRDAGQPTWLAALFFAPIANLAFFL
                     ALSFMPSLAELKREEGTPWPGVRAFDRWIPRNAAGSALVSIGATCLLGLFFTFLGTQA
                     IAQYGWGLFVGLPFCLGLFAVLTHSYHQQRSYAECVTVAVLPVAMLGAVLMLVAIEGV
                     ICIMMAAPLAFCLALLGGSLGYGIQAAHWGRRHAPAMLGMVLLFPPGFYGIEHFTRPQ
                     PGVFEVKSAIEIKAPPEKVWQKVVAFTEIPPPTETLFRFGVAYPIRAEIAGHGPGAVR
                     HCVFSTGPFVEPITVWDEPHLLRFSVTENPAPMNELTPYGHIEPKHLHGYFESHQGQF
                     LLTELPGGRTRVEGTTWYSHSLWPETYWHWWSDYIIHRIHLRVLNHIRMELGDS"
BASE COUNT          869 a         1172 c         1168 g          871 t
ORIGIN      
        1 tgctccgctg gccggcagcg aagtcgaagc tatcctgaag gacaaatccg acctctcgcc
       61 ggcggccatt cgcaccgccg cccagctcgc cgaaggcagc cccggctatg ccctcgaaat
      121 ggatgtcgaa aaagaatcac aacggcggcg cgaagccctg aaaattctgg atcgcgcctc
      181 gcgcggtgaa gaatttggtc aaatctttgc ccagacctcg gcattggcca aagaccgcga
      241 catttctttc gaggatcagc tttctgtttt ctatagccta ctttctgatc ttttggaact
      301 ttcttctgga attcatacac cacaaatccg caatccgccc ttgcgcaaag aattggaagt
      361 gacagcgcgc cactttagcg ttgactctat ccagcgggcc ctttctgggc ttgatcagct
      421 tgccgcaggc gctcgccgca acctgaacaa gcaactcggc ctcgaagcat ttgccgcgga
      481 tctttcgtcc taagccattg cgtgtcagcg gcgcggtaaa actttgtaaa gaaaatgtgg
      541 caaccgtagt cctgacaatt ttcatcccat tgccatgtaa cccctggcag gcaacccgcg
      601 tcatatgaac attgctgctc aaactccact cccttgggca ttgaggagtc ctttataatg
      661 gtaaatcgtc gtatgtttcg tccaagtttt accgaggcca aagagccgat tccggtgcgt
      721 cccaacaacc atcaaccgca tcagtcacat aacgggcctg ccccgaccaa gaaaccgacg
      781 ccgccggaaa tgactcatgc cgaaaatttc tattggatca agcagatgca gtcccgcacg
      841 cccatggtga tcgttatgga caatggcgaa tcgctgcacg gcatcgtgga atggtacgac
      901 cgcgactgca tcaagttgac ccggcatggc agcgccaacc tgatgatctt caagaagaac
      961 atcaagtatg tgtacaaaga aggcgaagag ggcggcagcg aagaataacg ctagccgttt
     1021 ttctttttcc gcttagtcgg tccggcaact ctagatttct tcggccctgc ttccgtggcg
     1081 ggcttgccgg ttagtacgtg tccgggtgcg gctccaaacg tcatcagcgc cttggccgcc
     1141 agcgcgccgt cctgtttggt cacatcacat tccacgacaa taaagttacg gccggcgcgt
     1201 aacacctttc cctctgcttt gagcttgccc tcggcgaccg gcagcaggta gttgatcttt
     1261 agttcgatgg tgacgacttc cgccccgcgc ggaataacgg tgtaggcggc gatcgccgcc
     1321 gcggtatcgg ccagcgccgc gattactccg ccgtgcatca cgttatggac ctgccggtga
     1381 tggtcctcgg taaccacctg cagaatcgcc ccgccggggt acagtttcgc cacctcaaat
     1441 cccatcaacg cgctgaattt actcagcttg acgcggctgc gggcgtattc gaggtgctcg
     1501 cgggtaaatt tcattccgcc tcctggtgca ctcattcctc ggcttcactc cttgcaaatg
     1561 tcacggtttt gcggcgccct gcgtctaaca gctcatccgg tttgcggtcc gggtgttctc
     1621 gttgcccgac attctagcag gccggccctc gcctgggtca gccggagcgg gtgtgtcagc
     1681 ctgacttgtt gagagttgtt tcgtcttcga ctatgcgtat cggcattact tgctacccca
     1741 cttatggcgg ctccggcgtc gtcgccaccg agcttggcca ggaactggcc gcgcgcgggc
     1801 acgatgtcca tttcatcagc tatgcgccgc caatccgaat gaatccgaac gatccgcaca
     1861 ttttcttcca cgaggtagaa gtcgcgagct atccgctgtt cgaccatccg ccgtacgcgc
     1921 tggcgctcgc caccaagatg ctggaggtgt ttgagtcgga agcgctggat ctgctgcatg
     1981 tacactatgc gattccgcat tcggtgagcg cgatgctggc tcgttcgatg gcagcgccgc
     2041 ggcgtctgcc attcatcacc acgttgcatg gaacggatat cacattggtt ggcagcaacc
     2101 gttcgtattt gccgatcacg aaattttcga ttgagcagag cgatggcgtg accgcgattt
     2161 cgcgctacct gctgaaccaa acgatgaagg aattcgacat taagcggccg atcgaagtga
     2221 ttccgaattt tgtgaattgc gatctgtata cgcggcagcc gaatctggcg cagcgcggca
     2281 aatgggctcc cgcgggcgag ccgattctga tgcacctttc gaatttccgg ccggtgaaac
     2341 gggttaccga ctgcgtggaa atcttcgcgc aggtgcgcgc gaagatgccg gccaagctgg
     2401 tgttgatcgg cgatgggccg gaacgcggag ctgcggaata tctggtgcgc aaaaagaaac
     2461 tgcagaaaga tgtgatattt ctcggcaagc aggaccaagt gtatcgcctg ctgtctgatg
     2521 cagatctttt cctgctgcct tcacagttgg aatcgtttgg cctggctgcg ctcgaagcga
     2581 tggcctgcga agttcctgta atcgcgacga acgtaggtgg cgtaccggaa gttatcgaac
     2641 acaatgtgga tggctatctc gtagaacctg gcgacgttgc cgctgcgtca aaatatgcga
     2701 ttgagttgct atcacgcgct gatcgcggcc gcgaaatggg tgaacttgcc cgcgtcaacg
     2761 ccaaaaagaa attctgcgcg aatgacgtga ttccttccta cgaacgctac tatcagcggg
     2821 ttctctcgga aacccgtgct gcttcggcct gatctgtagc ggccacattt atgtgtctaa
     2881 ccctccgaat gatggccatc ggaaggtggc cgttacgaat ctcccagttc catgcggatg
     2941 tgattgagga cgcgcaggtg gatgcgatga atgatgtagt cggaccacca gtgccagtag
     3001 gtttcgggcc acagggagtg ggaataccat gtggtgcctt cgacgcgggt gcggccgccg
     3061 ggtaattcgg tgagcaggaa ctggccctgg tgcgattcga aatagccatg caggtgtttg
     3121 ggttcaatgt ggccgtaggg agtgagttcg ttcatcggcg cgggattttc ggtaacgctg
     3181 aagcggagca gatgcggctc gtcccacacc gtaatcggct cgacgaacgg gccagtagag
     3241 aacacgcagt ggcggactgc gccggggccg tggccggcaa tttccgcgcg aattggataa
     3301 gcaacgccaa agcgaaaaag agtctctgtc ggcgggggaa tttcggtgaa agcgaccact
     3361 ttctgccaaa ctttttccgg cggcgccttg atttcgatgg cgctcttcac ttcgaagaca
     3421 ccgggctgag gccgtgtgaa gtgctcgatg ccataaaatc ccggcgggaa gagaaggacc
     3481 attccgagca ttgcgggcgc gtggcggcgt ccccaatgcg ccgcctgaat cccgtacccc
     3541 aagctgccgc ccagcaacgc caggcagaat gccagcggcg ccgccatcat gatgcagatc
     3601 acgccttcga ttgccacaag catcaggacc gctcccagca tggccacagg caggacggcc
     3661 acggtgacgc actctgcgta gcttcgctgt tggtggtagc tatgggtcag caccgcaaac
     3721 aggcctaaac agaacggcag gccgacaaag aggccccaac cgtattgcgc aattgcttgt
     3781 gtacccagaa acgtgaagaa gagccccagc aaacaggttg cgccgatgct gaccagggcg
     3841 ctgcctgcgg cgttacgcgg aatccaacga tcgaaggctc gaacgccggg ccacggtgtt
     3901 ccctcttcgc gcttcaactc ggctagggac ggcatgaagc tcagggcgag gaagaaagcc
     3961 aggttggcga taggcgcgaa gaatagagcc gccagccagg taggctgacc cgcatcgcgg
     4021 agccgcttta ccgtcatcgc caggcccaac caaatgaagg gtaaggccac aaaaagcatg
//
LOCUS       DASTPM010000688         4794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_399683, whole genome shotgun sequence.
ACCESSION   DASTPM010000688 DASTPM010000000
VERSION     DASTPM010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399683"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1211
                     /locus_tag="VFR42_12155"
     CDS             261..1211
                     /locus_tag="VFR42_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369961.1"
                     /translation="MNYPWLAYGADFGRSPWGHNGVSLESARAAVAADFAAIRSSGVS
                     VVRWFLLCDGRSGIRYENEIPAAPDEFLFADVAAALDLAAQSDVRICFSLFDFLWLQT
                     PESTTAAANRTFPGRNALQFAGGREALLQRILIPLFRQFRAHPGLFAWEIVNEPEWAI
                     PEFQRSPKATLPLADARAFFAEIAGAIHQESAGIQVSLGSARLQWVRAWSKIGLDHLQ
                     AHYYPQLERDQPFSLSQQLAELANLAQPLWIGELPANDPSSPDYSLAAALAACRDAGL
                     SGAGIWRWRPPEPGGSDVAFGSVQPEILSAWLSQASEFRV"
     gene            1220..2500
                     /locus_tag="VFR42_12160"
     CDS             1220..2500
                     /locus_tag="VFR42_12160"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0369962.1"
                     /translation="MKASAFIERLAICLAVAVCSVTLLQAQEEKSQQPKEEEVRGAPA
                     EPDDAAEVRAQITAVEKMIPTYVDRGAVFYFLAASKMHLGETREALDRLKQCMALEEG
                     FDPSGASEFVGLRSERAFSEMVDRAKTRFPAVALSRKALVTEEKDLIPEGLAWDSVRE
                     VFYLGSLHRRKIVQITTDRHVSDFLQPQQDAVLPVLGIRMHPNDGTIWANTWEEKPRA
                     SRSQLLHVDASGKILVRFAPSDSAQHGFNDLVVRKAGDVFVTDSLANQVFRFDPDTKS
                     FSPLRLHRQLYYPNGIALAEDDRTLYIADALGVLNYDVASSSSVDVAPGAHNTLAGVD
                     GLYWYRGSLVAIQNGIGTPRVAVHKLAGDGTRVAKVTILEFRTTLSVLPTTGAVRGSD
                     FYFIANSQIDNLNGDKILDVTRLEPVRIAVVHLP"
     gene            2574..2852
                     /locus_tag="VFR42_12165"
     CDS             2574..2852
                     /locus_tag="VFR42_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369963.1"
                     /translation="MNLPLANTRARVVLLVCCGALALLLGFLAVRNAIAAHYLGRDTR
                     EGYEHAVRLEPHNPRNWYLLGRSYLYDLEQPDPVQAVSALRKSVQLDP"
     gene            2865..3881
                     /locus_tag="VFR42_12170"
     CDS             2865..3881
                     /locus_tag="VFR42_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0369964.1"
                     /translation="MLDLAIAYDGEGDTAQARATLVSAQRVYPLSADVAWSYGNFLLR
                     QGEQKAAFSQLHTALELDPKRAAEAFSRASQLQPDASTVLDRVVPATPATYVPILHML
                     SDAGDMETAQQVWFRLIGLHQEVPLREVVPFFDALIHERRPDQAARLWLQGVAIMENP
                     PPDDPAGSLLWDGGFESGLRGGGFSWRFTPVSRDVQISFDRAEKHAGEQSLRILFNGR
                     ENLKFEDACHSIVPEPGKSYLLAGWVKTQSLTSSEGVRLQINVFTNAGVVSTQSGEIH
                     GTQPWTQLQLVWTAPQESGFGNVCIDRNMSDMPGSDIQGAAWIDDVVMIPAGAAGAAV
                     APRP"
     gene            3878..>4794
                     /locus_tag="VFR42_12175"
     CDS             3878..>4794
                     /locus_tag="VFR42_12175"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU0369965.1"
                     /translation="MKLIRIGIFATVAFAVLSHGAVEPWARGILQCASGLLLVVWALQ
                     FYFADEEREIVFPLLLFPMFALTLLVLVQWAFHLTASRFSTRTELLLLLSMVILIFLA
                     AQAFRSLADWRAFAWFTMLLAFFVAGFGILQHLTFNGKLYWFREMRYGGIPFGPFANR
                     NHFAGFVELVLPMSLVPLLLGRVRRDRLVIVGILAVIPLSAILLAASRGGIISLFAEL
                     TFLFLVVLLRRAGGRHLYVGGLVLVAAFATVSWLGVNEILTRFSSLQSLEVTQSKRAS
                     MRHGAWKIFLDHPVLGTGLGTLQQVYPPY"
BASE COUNT          885 a         1430 c         1374 g         1105 t
ORIGIN      
        1 acaacacgtc ctcccgttct atccttttct ggactgtagc cgttatcgtt catagaaggc
       61 ttatttctcc tggttccggc cgccaccgat tctctaaggt ctttaaaatc agcacttgca
      121 aaaaacgctt gaaaacaagg agctttaaac acctttagaa tcacccgtta tcaaaaaaac
      181 ggggggggcg gcctgtatca agggtggccc tcgtcgttta tcatccctga atggcgccac
      241 gtccggattt ctttctcgct gtgaattacc cttggctcgc ctacggcgcg gatttcggcc
      301 gctcgccctg gggacataac ggcgtaagtc tggaatccgc tcgcgcagcc gtagccgccg
      361 atttcgcggc cattcgctct tcgggagttt ccgtcgtccg gtggttcttg ctctgtgatg
      421 gacgcagcgg catccgctac gaaaatgaaa ttcccgccgc tcccgacgaa tttctctttg
      481 ccgatgttgc ggccgccctc gacctcgctg cacaatccga cgtgcgcatc tgtttttcgc
      541 ttttcgattt tctctggctg caaactcccg agtcaacaac agccgcggcc aatcggacgt
      601 ttccagggcg aaatgccttg cagttcgcgg gcggccgcga agccttgctg cagcggatct
      661 tgatcccgct gttccgccaa tttcgcgcac atcccggact atttgcttgg gaaattgtca
      721 acgaaccgga atgggccatc ccggagtttc agcgctctcc aaaagccact ttgcctctgg
      781 ccgatgcgcg tgcgtttttt gccgaaatcg caggcgctat ccatcaggaa tctgcaggaa
      841 tacaagtgtc acttggttcc gctcggctgc aatgggttcg cgcgtggagc aaaatcgggc
      901 tcgatcacct gcaagctcac tactatccgc agctcgagcg cgatcagcct ttttctcttt
      961 cgcagcagct ggcagaactc gcaaatctcg cgcaaccgct gtggatcgga gaactgcccg
     1021 ccaacgatcc atcgagcccg gactattctc ttgcagctgc gcttgctgcc tgccgcgatg
     1081 cgggcctctc cggtgccggc atttggcgat ggaggccccc cgagcccggc ggctccgacg
     1141 tagctttcgg ctccgttcag ccggaaattt tatcggcctg gctatcgcaa gcctcggagt
     1201 ttcgcgtcta atggaagcaa tgaaagccag cgcattcatc gaacggctcg caatctgtct
     1261 ggcggtggcc gtttgctccg taaccttgct gcaagcgcaa gaagaaaaat ctcaacaacc
     1321 caaggaagaa gaagtccgcg gcgctcccgc cgaacccgat gacgccgcgg aagttcgcgc
     1381 gcagattacc gctgtcgaaa aaatgattcc gacttatgtt gatcgcggag cggtgtttta
     1441 ttttctcgcc gcttccaaaa tgcatcttgg tgaaacgcgc gaagcgctcg accggctgaa
     1501 gcaatgcatg gcgctcgaag aaggttttga tccttccggc gcatcggagt tcgtgggctt
     1561 gcgcagcgaa cgtgccttca gcgagatggt ggaccgcgcc aaaacccgct ttcccgcggt
     1621 tgcactatcg cgcaaagccc tggtcaccga agagaaagat ctgattccgg agggactggc
     1681 gtgggactcc gtacgggagg ttttctatct cggcagcctg catcgccgga agattgttca
     1741 gatcacaacc gaccgccatg tcagtgattt cctccagccg cagcaggacg cagttcttcc
     1801 ggtgcttggg atccgcatgc atcccaatga cggcacgatt tgggccaaca cctgggaaga
     1861 aaaacctaga gccagccgct cgcaacttct acatgtggac gccagtggaa aaattctcgt
     1921 gcgttttgcg ccgtctgatt ccgcacagca tggatttaac gacctggtgg tacgtaaagc
     1981 gggcgatgtc tttgtcacag atagcctggc caatcaggtt tttcgtttcg atccggacac
     2041 caagtcgttt tccccgctgc ggctgcaccg gcaactctat tatcccaacg gaatcgcgct
     2101 cgccgaggac gatcgcaccc tgtacattgc agatgcactc ggagtgttga attacgacgt
     2161 cgcatcttcc tccagtgtcg atgttgcgcc gggcgcacac aacacgctcg ccggggtgga
     2221 tggcctctac tggtatcgtg gcagccttgt tgcgattcaa aacggcattg gcacgccgcg
     2281 cgtcgccgtt cacaagctcg caggagacgg aacgcgggtc gcaaaagtta ccatcctgga
     2341 atttcgcacc acgctcagcg tgttgccaac gaccggcgcc gttcgcggtt ccgatttcta
     2401 tttcatcgcc aacagccaga ttgacaacct caacggcgac aaaatcctgg acgtcactcg
     2461 gctcgagccc gtgcgcatcg cggttgttca tcttccgtga agcctccaca acttccggtc
     2521 acctggactt ttttcttgcc tccgtgcctc gcgcttaggc agagtaggca aggatgaatc
     2581 tgccactcgc gaatacccgc gcccgcgtcg tgttgctggt gtgctgcggc gcgcttgcct
     2641 tgctcctcgg ttttctcgcc gtgcgcaatg ccattgccgc tcactacctg ggtcgagata
     2701 cgcgcgaagg ctacgagcac gcggtgcggc tcgagccgca taatccgcgc aactggtatc
     2761 tcctgggccg ctcctatctg tatgacctgg agcagcccga tcccgtccag gccgtcagcg
     2821 cgttgcgcaa atcggtgcaa ctcgatccct agtcggcgga ggccatgctc gatcttgcca
     2881 tcgcctacga cggtgagggc gatacggcgc aggcacgcgc cacgctggtt tccgcgcaac
     2941 gcgtttaccc cctgtcagcg gatgtggcgt ggagctacgg gaacttcctg ctgcgccagg
     3001 gagagcagaa agcagcgttc agtcaactgc ataccgcgct cgagctggat ccgaaacgcg
     3061 ccgcggaagc tttttcgcgc gcctcgcagt tgcagccaga cgccagcacg gtgctggaca
     3121 gagtcgttcc ggctacaccg gccacgtatg tgccgatttt gcacatgctg tccgatgcag
     3181 gtgatatgga aacggcacag caggtttggt ttcgcttgat cgggctgcat caagaggttc
     3241 cgctgcgcga agtcgtgccg tttttcgatg cgctgattca tgaacgaaga cctgaccaag
     3301 ccgcccgact ctggctacaa ggcgtcgcta tcatggagaa cccgccgccc gacgatcccg
     3361 ccggttctct cctgtgggac ggaggcttcg aatccggctt gcggggcgga gggttcagct
     3421 ggcgattcac gccggtcagt cgcgacgtgc aaatttcttt cgatcgtgcg gaaaaacatg
     3481 ccggggagca atcgctgcgc attctgttca acggccgcga aaaccttaag ttcgaggatg
     3541 cctgccactc cattgtgccg gagcccggca agagttatct cctggcgggg tgggtgaaga
     3601 cgcaatcttt aacaagcagc gagggcgtac gtctgcaaat taacgtcttc accaatgcag
     3661 gcgtggtgtc cacgcaaagc ggcgagattc atggaacgca gccatggaca cagctgcaac
     3721 ttgtttggac cgctccgcag gagtcgggtt tcggaaacgt ttgcatcgac cgcaacatga
     3781 gcgacatgcc cggaagcgat atccagggcg cggcctggat cgatgatgtg gtcatgattc
     3841 cagccggcgc tgcgggagcc gcggtggcgc cccggccatg aagctgatcc gcatcggaat
     3901 ttttgcgacc gtcgcatttg ccgtattgag ccacggcgcc gtcgaaccgt gggcgcgcgg
     3961 aattctgcag tgcgccagcg ggcttttgct ggtggtgtgg gccttgcaat tttacttcgc
     4021 ggacgaagag cgcgagattg tgttccccct cttgcttttt cccatgttcg cactgaccct
     4081 gctggtcctc gtccagtggg cattccatct cacggcttcg cgattttcca cgcgcacgga
     4141 actgctgcta ttgctcagca tggtcattct gatttttctg gcggcgcagg ctttccggtc
     4201 gctggcggac tggcgcgcct tcgcatggtt cacgatgttg ctggcatttt ttgtcgcggg
     4261 cttcggcatc ctgcagcacc tgacgttcaa cggaaaactg tattggttcc gcgagatgcg
     4321 ttatggcggc attccttttg gaccgttcgc caaccgcaat cactttgcgg gattcgtgga
     4381 actggtgttg ccaatgtcgc tggtgccctt gttgctcggg cgagtgcggc gcgaccggct
     4441 ggtgattgtg ggaatcttgg cggtgattcc gctgagcgcc attttgcttg cggcatcgcg
     4501 gggcggaatc atcagtctct ttgcggaatt gacatttttg tttctggtgg tgctgctgcg
     4561 tcgcgcgggc ggccgccatt tgtacgttgg cgggttggtt ctcgtggcag ccttcgccac
     4621 tgtgtcctgg ctcggtgtca acgagattct cacccgtttt tcttcgctgc aatcgctgga
     4681 agtgacgcag agcaaacgcg cttcgatgcg ccatggagcc tggaaaatat ttctggatca
     4741 cccggtcctc ggcaccgggc tgggaaccct gcaacaggtt tacccgcctt acga
//
LOCUS       DASTPM010000689         4401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_197860, whole genome shotgun sequence.
ACCESSION   DASTPM010000689 DASTPM010000000
VERSION     DASTPM010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197860"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /gene="pyrH"
                     /locus_tag="VFR42_12180"
     CDS             complement(<1..715)
                     /gene="pyrH"
                     /locus_tag="VFR42_12180"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0369966.1"
                     /translation="MAARKTERKSSKKKPVYKRILLKLSGESLQGPQGYGVHGETLKS
                     IAKELKEVHDLGVQIAVVVGGGNIFRGGRQKGFEIDRATGDYMGMLATVINAMALQDS
                     LEKVGVFTRVLSAIEMHEVCEPFIRRRAMRHLEKDRVVIFGAGTGNPYFSTDTAAALR
                     AMEIKADVILKATRVEGIYDADPEKVAGAKLFQQITYRDVLHQDLKVMDSTAISLCMD
                     NGMPIVVFSMNEFGNIKRVV"
     gene            complement(842..1486)
                     /gene="tsf"
                     /locus_tag="VFR42_12185"
     CDS             complement(842..1486)
                     /gene="tsf"
                     /locus_tag="VFR42_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0369967.1"
                     /translation="MSTPNAAIEIPAKLVKELRDRTNAGFNDCRAALVEAAGDIEKAI
                     AVLRKKGQAAAAKKATREATEGLVGSYIHAGGKIGVLVEVNCESDFVARTEAFQQLCH
                     DIAMHIAALDPKYVRREEVTADMLDKEREIYKAQALATGKPEVVVDKIVTGKMEKFYE
                     ENCLYEQHYIKDEGQTIGEMVNAAIAKLGENIAIKRFARFKVGEVTGSETAPKS"
     gene            complement(1674..2546)
                     /gene="rpsB"
                     /locus_tag="VFR42_12190"
     CDS             complement(1674..2546)
                     /gene="rpsB"
                     /locus_tag="VFR42_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0369968.1"
                     /translation="MAVEITMKELLEAGVHFGHQTRRWNPKMKEYIFGERNGIHIIDL
                     QKTLKMFREASRFVSDLSGQGKAVLFVGTKRQAQEAVAEEAKKCGAFFVNHRWLGGTL
                     TNWATLQKSIKRLKLLKAMTEDGRMEQLSKKERARLDREMKHLFQNLEGIENMSQLPD
                     AMFVIDSNAEEIAVKEARRMGVPVVSVVDTNCDPDVVDWIIPGNDDALRAIRLFTSKI
                     SESVAEGKHNYEQSQVGEQKSAADQALADGVEYVDTSAYEQYEKQEGDFVEGAEEAAA
                     VPAEATVPPDDTQA"
     gene            complement(2815..3237)
                     /gene="rpsI"
                     /locus_tag="VFR42_12195"
     CDS             complement(2815..3237)
                     /gene="rpsI"
                     /locus_tag="VFR42_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU0369969.1"
                     /translation="MSTAESPLVGSNKTWFHGTGRRKESVARVFHRAGSGKFTVNRRT
                     VDSYFPNHAWKHDATEPLKFTNLADQVDVVVTAHGGGTGGQAGAVRMGLSRAISRYNP
                     ELRPALRKNGFLTRDSRMKERKKYGQKGARKRFQFTKR"
     gene            complement(3488..3937)
                     /gene="rplM"
                     /locus_tag="VFR42_12200"
     CDS             complement(3488..3937)
                     /gene="rplM"
                     /locus_tag="VFR42_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU0369970.1"
                     /translation="MRTYVAKGEEAEALKVGAAWFVVDATDKVLGRLATKVARVLIGK
                     DKPSFTPYLDSGDHVIVINADKIRMTGNKVDQKMYYSHSGYPGGLKEVPAKRVRETKP
                     EWVVREAIVGMLPKNKLRARRAKKLRVYRDAAGLSRHTGQKPQPLAV"
     gene            complement(4190..>4401)
                     /locus_tag="VFR42_12205"
     CDS             complement(4190..>4401)
                     /locus_tag="VFR42_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369971.1"
                     /translation="KNIHITERTNLEFQYVVTNLFNHPVFSDPAAGDAQTGLGVDPTD
                     ASSFGVVNTQGNNPRQMQFGLRFTF"
BASE COUNT          883 a         1292 c         1204 g         1022 t
ORIGIN      
        1 tgaccacgcg cttgatgttg ccgaactcat tcatactgaa caccacgatc ggcattccgt
       61 tatccatgca cagcgaaatc gccgtcgaat ccatcacttt caaatcctgg tgcagcacgt
      121 cgcgataagt aatctgctgg aacagtttcg cgcctgcgac tttttccgga tcggcgtcgt
      181 agattccttc cacacgcgtg gcttttagaa tcacgtcggc tttgatttcc atcgcgcgca
      241 aagccgccgc ggtgtccgtg gaaaaatacg gattcccggt gcccgcgcca aaaatgacga
      301 cgcgatcctt ttccagatgc cgcatggcgc gccgccggat aaacggctcg cacacttcgt
      361 gcatctcaat cgcgctcagc acccgcgtga acacaccgac tttttcgagc gaatcctgca
      421 aagccatggc gttgatcacc gtggccagca tgcccatgta atcgccagtg gcgcgatcaa
      481 tctcaaatcc tttttggcgg ccgccgcgaa agatatttcc gccaccaact accacggcga
      541 tctgcacgcc caggtcgtgc acttccttca attccttggc gatcgatttc agcgtctcgc
      601 cgtgaactcc atatccttgc ggcccttgca gcgactcgcc cgacagcttc aacaaaatac
      661 gtttgtaaac cggtttcttc ttggagcttt ttcgttcggt ttttcttgcc gccatgtcgc
      721 tccgttcaaa gtgtggaaag cgcgagcaaa acttcacgcc aaatccaccg aattgtaaca
      781 aaaaagcgcc ccggatgttc cggggcgctg ttgaaacctg caaattttcg cgtcaaccaa
      841 gtcaggactt gggcgcggtt tcgctgccgg tgacttcgcc aaccttgaag cgggcgaagc
      901 gcttgatggc gatgttttcg ccgagcttgg cgatggcagc attcaccatt tcgccgatgg
      961 tctggccctc gtccttgatg tagtgctgct cgtagaggca attttcctcg tagaactttt
     1021 ccattttgcc ggtcacgatt ttgtccacca cgacttcagg cttgccggtg gcgagcgcct
     1081 gcgctttgta gatttcgcgc tccttgtcga gcatgtcggc agtcacttcc tccctacgca
     1141 cgtacttcgg gtccagcgcg gcgatatgca tggcgatgtc gtggcaaagc tgctggaaag
     1201 cttcggtgcg agccacgaag tcggactcgc agttcacttc aaccagcacg ccgatttttc
     1261 cgccggcgtg aatgtagctg ccgactagtc cttcggtggc ctcgcgcgtg gcttttttgg
     1321 cggccgcggc ttgtcccttc ttgcggagaa ccgcaatggc cttttcaatg tcgccggccg
     1381 cttcgactaa ggcggcgcgg caatcgttga agcctgcgtt agtgcggtcg cgcaattcct
     1441 ttaccagctt ggctggaatt tcaatagctg cgtttggtgt gctcattctt tcttctctct
     1501 ttttcgatcc gctcaacatt ccgcctcagc aaatcggcgg ctacacttct ctcgatcgac
     1561 gccgcagaat atcggggaag tattgcggcg ctgttacaac ttcgctctta taaaagggcc
     1621 cgctccaagt cgcctcggaa cgggccgcat ttcttgccag tgttccggcc aaattatgct
     1681 tgcgtgtcgt cgggggggac ggtggcttcc gcgggaacgg ccgcggcttc ttcggcgcct
     1741 tcgacgaaat cgccttcctg cttttcgtat tgttcgtagg cgctggtgtc cacgtattcg
     1801 acgccgtcgg ccagagcctg gtccgcggcg gatttctgtt cgccgacctg ggactgctcg
     1861 tagttgtgct tgccttcggc taccgactcg gaaattttcg aagtgaacag gcggatggcg
     1921 cgcagcgcgt catcgttgcc cgggatgatc cagtccacca cgtcgggatc gcagttggtg
     1981 tccacgacgg agacgacggg aacgcccata cggcgcgctt ccttgaccgc gatctcttcc
     2041 gcgttggaat cgatcacgaa catggcatcg ggcagctgcg acatattttc gatgccttcg
     2101 agattctgga aaaggtgctt catttcgcgg tccagccgtg cgcgctcttt cttggagagc
     2161 tgctccatgc ggccgtcttc ggtcatggcc ttgaggagct tcagccgctt gatggacttc
     2221 tggagggtcg cccaattggt cagcgtgcca ccgagccagc ggtggttcac gaaaaacgcg
     2281 ccgcactttt tggcttcttc cgccacggct tcttgcgcct ggcgcttggt accgacaaac
     2341 aaaactgctt tgccctgccc cgaaagatcg ctcacgaagc ggctggcttc gcgaaacatt
     2401 ttcagcgttt tctgcaggtc aatgatgtga attccgttgc gctcgccgaa gatgtattcc
     2461 ttcatcttgg gattccagcg ccgcgtctgg tgaccgaagt ggactccggc ttccagcagc
     2521 tctttcatgg tgatttctac tgccactttt cctccttgtt agacagctgc ttgctgtgag
     2581 tgaccgctgc aggaggctac ctaaaggtgg ccgctacatg gtcctgcaca gaacagcctt
     2641 ttgccggcgg cttgccggcc gtgatctcag tgagagcttg cgctcgcact ggagctcgtc
     2701 tcatgccgcc cctaaaaacg gcggcagtga aattcttaag tacgcccgcc tcagaaggcg
     2761 gccgcttacc accgagcgca aggaaagcgg cagcagagct gccgcacgcc taaactagcg
     2821 cttggtgaac tggaagcgct tgcgggcgcc cttctgccca tacttcttgc gttccttcat
     2881 gcgcgaatcg cgcgttagaa agccattctt gcgcagcgcg ggccgcaact cggggttgta
     2941 gcgcgaaatc gcgcgggaca agcccatgcg caccgctcca gcctgaccgc cagtcccgcc
     3001 gccatgcgcc gtgaccacca catccacctg gtcggcgaga ttggtgaact tgagcggctc
     3061 ggtggcgtca tgcttccacg cgtgattcgg aaaataggaa tcgacggtgc ggcggttgac
     3121 ggtgaacttg ccggatccag cgcgatgaaa aacgcgcgcg accgattcct tgcggcgtcc
     3181 tgtgccgtgg aaccaggtct tattgctgcc caccaagggc gattcagcag tgctcaacga
     3241 tcctcctcaa atccgggtca aaacccaggg gccaacgagc ggcaaaacga tggtgcgacc
     3301 gcttcccagc ctaaagctac ttcgctacca gtgatccctt tttcaagccc aggctttgtt
     3361 gaaacaaacc ctgaaacccg gaaggcctgc aaagacctac agaggaacaa caagtgcgga
     3421 aacctctaaa aagtcctttt cgcaagtatt cattctaaaa gacttagcga aaaggctgca
     3481 aacaaactca aacggccaga ggctggggct tttggccggt gtggcgcgac aaaccggcag
     3541 cgtcgcggta cacacgcaat ttcttggcac ggcgggcgcg aagcttgttc ttcggcaaca
     3601 tgccgacaat ggcttcgcgc acaacccatt cgggcttggt ctcgcggacg cgcttggccg
     3661 gaacttcctt caagccgccc ggatagccgc tatgcgaata gtacatcttc tggtcgactt
     3721 tattaccagt catgcgaatc ttgtcggcat tgatcacgat cacgtgatcg ccggaatcga
     3781 gatacggcgt gaaggaaggc ttgtccttgc cgatcaaaac gcgagcaact ttggtggcga
     3841 gccgaccgag cactttatcg gtggcatcca caacaaacca cgcggcgccg actttgagcg
     3901 cttccgcttc ttcacccttg gctacgtacg tcctcatgga accgaatctg tcctttctaa
     3961 ctcaggggtt gtaaaacaaa ccctgaaacc tgtcacatgc ttcgcgattc tcgagcgggt
     4021 agccacacgt agaccgctgc agtacagaca attggtaaat tgtatggggg ctcgggcgaa
     4081 aagtcaacga aacacgcgga tagccagtgt agacgtggaa aagcttaagc gacacagacc
     4141 aaggagagag ccgatggctc tctccttttg aaaaatggat cgcttgagat tagaaagtga
     4201 accggagacc gaactgcatc tgccgtgggt tgttgccctg ggtgttaacc acgccgaagg
     4261 atgacgcgtc agtagggtca acaccaaggc cagtctgcgc gtcccctgcg gctggatcgg
     4321 agaagaccgg gtggttgaaa agattggtga cgacgtactg gaattcaaga ttggtgcgct
     4381 cggtaatgtg aatgttcttt a
//
LOCUS       DASTPM010000690         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1034073, whole genome shotgun sequence.
ACCESSION   DASTPM010000690 DASTPM010000000
VERSION     DASTPM010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034073"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VFR42_12210"
     CDS             <1..1163
                     /locus_tag="VFR42_12210"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU0369972.1"
                     /translation="LDSVGIGALPDAADYGDVGRTTLGHIAESRPLKIPMLVSLGLAN
                     IAPLAHLASAALPLAAYGKGATHSPGKDTTTGHWEMAGVWLDQAFPVYQHGFPPEIIS
                     AFQARIGRKTIGNKPASGTEIIKELGEEHVRTGKPIVYTSGDSVFQIATHEEVVPIAD
                     LYKMCEIARELLQGPHRVGRVIARPFTGAPGHFTRTPRRHDYAVDPPMPMLLDVLAEK
                     NIPVFGIGKIHDIYNGRGVSDYVSTTCNSDGMAKLTSAVRERPAGLIFCNLVDFDMLY
                     GHRKDVEGFARSLEEFDAQLTQFLALLTRDDLLMITADHGCDPDPRWETTDHSREYVP
                     ILAYSPSVAQTIPPRPTFRNLGTRSTLADMGQTIAQNFGGTIPHGSSFLDQL"
     gene            1313..>3328
                     /locus_tag="VFR42_12215"
     CDS             1313..>3328
                     /locus_tag="VFR42_12215"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0369973.1"
                     /translation="MASGSLIGQTISHYLMVEKIGAGGMGVVYKAKDTRLARFVALKF
                     LPDKTAHDPLALERFRREARAASVLNHPGICTIYDIGEQGGRAFIAMEFIDGETLRSR
                     IQGKPLALQEILKLGIQIAEALDAAHTEGIIHRDIKPANIFVTRRAQAKVLDFGLAKL
                     VPTGLAGTDAELAAESAHSTSIVGIISGTPSYMSPEQIRGDDLDPRTDIFSLGVVLYE
                     MATGRQAFGGRTGGAIIEAVLTRPPVSVRSINPHIPPRLENIIDHALHKDREQRYQLA
                     ADLLADLQQLNRETDFSRQGPNGGTQSIPVSTATPAFPSGSRRPRTSTRQKSVLRAEC
                     PARGISSLAVFPFENLTRDPENEYLSDGIPGSLINNLATVPRLRVIAQSTVFRYKGRQ
                     IDPQAVGRELHVRAVLTGRMMQSGGSLRVGTELVDVATGTQLWGEQFDRKPGDIFAIQ
                     DEISHEISEKLRLQLTRAEKKRLIKRHTQDPEAYRLYLQGRHHWNRWTEEGFYKAIGY
                     FLQAVEKDPGYALAFAGVADSYVLLGWNSYLPPKDAFPQGRAAAMNALQLDPGLGEAH
                     ASLAAVLWLYDWQWPEAQSEFKRSLALNPTYPTANHWHAEFVMTMGLPIEAIAKMKNS
                     QELDPLSLIINVAIGWAFYMARRYDEAIEQLLRTVELDPHYPVTYWIL"
BASE COUNT          689 a         1159 c          832 g          648 t
ORIGIN      
        1 tcctcgacag cgtcggcatc ggcgctctcc ctgacgccgc cgactacggc gacgtcggcc
       61 gcaccacact cggccacatc gcggaatcgc gccctctcaa aatccccatg ctagtctcgc
      121 tcggcctcgc caacatcgcg ccgcttgcgc atctcgcctc ggccgcgctg cccctcgccg
      181 cctacggcaa aggcgccacg cactcaccag gcaaagacac cactaccggc cactgggaaa
      241 tggccggggt gtggctcgat caggcttttc cggtgtacca gcacggcttc ccgccggaaa
      301 tcatcagcgc tttccaagcg cgcatcggcc gcaaaaccat cggcaataag ccggcctccg
      361 gcaccgaaat catcaaggag cttggcgaag agcacgtccg caccggcaaa cccatcgttt
      421 atacttccgg cgacagcgtc ttccagatcg ccacccacga agaagtcgtc cccatcgccg
      481 acctttacaa aatgtgtgaa atcgcccgtg aacttctgca ggggccgcac cgcgtcggcc
      541 gcgtcatcgc ccgcccgttc accggagcgc ccggccactt cacgcgcacg ccacgccgcc
      601 atgattacgc cgtcgacccg cccatgccca tgctgctcga cgtgctcgcc gaaaaaaaca
      661 ttcccgtttt tggcatcggc aaaattcacg atatttacaa tggccgcggc gtctccgact
      721 acgtctccac cacttgcaac tctgacggca tggccaaact cacctccgct gttcgcgaac
      781 gcccagccgg cctcatcttc tgcaacctcg tcgacttcga catgctctac ggccatcgca
      841 aggacgtcga aggattcgcc cgctcgctcg aggaattcga cgcgcagctc actcaatttc
      901 tcgcgctcct cactcgcgac gacctcctga tgatcaccgc cgatcacggc tgcgatcccg
      961 acccgcgctg ggaaaccact gaccactctc gcgaatacgt ccccatcctc gcctactctc
     1021 cctccgtagc gcagacaatt ccccctcgtc ccaccttccg caatctcggc acccgttcta
     1081 cgctcgccga catgggccaa accatcgcgc aaaacttcgg cggcaccatc cctcacggct
     1141 ccagtttcct tgatcagctt tagctcgttc cgtcatttcg agctaaatcc gttggcggac
     1201 gattcgagcg acttgtggtt tgcctggaat tccgctgcat tcaatccacc ttcgccgccg
     1261 aaatcgctat tcgttgactt acttcgcgct tgccaactaa catgacggca ccatggcgtc
     1321 cggctctctc attggccaga ccatctcgca ttacctcatg gtcgaaaaaa tcggtgccgg
     1381 aggcatgggg gtcgtctata aggccaagga cactcgcctc gcgcgttttg tcgccctcaa
     1441 attcctgcct gacaaaaccg cccacgatcc gctggccctg gagcgctttc ggcgcgaagc
     1501 gcgagctgcc tccgttctaa accatcccgg catttgcacc atctacgaca tcggcgaaca
     1561 gggcggccgc gcttttatcg ccatggaatt catcgacggt gaaaccctgc gcagtcgcat
     1621 ccagggcaag ccccttgccc tgcaggaaat cctgaagctt ggcatccaga tcgccgaagc
     1681 tctggatgcg gcgcacacag aaggcatcat tcaccgcgat atcaagcccg cgaatatctt
     1741 cgtcaccagg cgcgcacaag cgaaagtgct cgattttggc ctcgccaagc ttgttcccac
     1801 cgggctcgcc ggcactgacg cggagctcgc cgctgaatcg gcacactcca cttcgattgt
     1861 tggaattatc agtggcactc cctcctacat gtcgcccgaa cagattcgcg gcgacgatct
     1921 ggacccccgc actgacatct tctcgctcgg tgtcgtcttg tatgaaatgg cgacaggacg
     1981 ccaggcgttt ggcggccgca ccggcggcgc aatcatcgag gccgttctga ctcgcccacc
     2041 agtttctgta cgcagtatca acccccatat cccgccgcgg cttgaaaaca tcattgacca
     2101 cgcgttgcat aaggacagag agcagcgcta ccagctagcc gccgatctcc tagcagacct
     2161 ccaacaactg aatcgcgaaa cggatttcag ccgccagggt ccgaatggag gcacgcaatc
     2221 catcccggta tcaaccgcaa cacctgcgtt ccccagcggc tcgcgcaggc cacggacttc
     2281 gacaaggcaa aaatctgtgc tccgtgccga gtgcccggca agaggcatta gctcactcgc
     2341 cgtctttcct tttgaaaatc tcactcgcga ccccgagaac gaatatctca gcgacggcat
     2401 ccccggcagt ctgatcaaca atctggctac cgtgccgcga ctccgcgtaa tcgcacagag
     2461 cacggtcttt cgctacaagg gacgccagat cgacccgcaa gctgtcggtc gcgagctcca
     2521 tgtccgcgcc gtcctcactg gcagaatgat gcaaagcggt ggctcgttgc gcgttgggac
     2581 tgagctagtc gatgtggcga cgggcaccca attatggggt gagcaatttg atcgcaagcc
     2641 cggcgacatt tttgcgattc aggacgaaat ttcccacgag atatcggaga aattgcgcct
     2701 acagctgacg cgtgcggaaa agaagcggct tatcaagcga cacacgcaag atcccgaagc
     2761 ctaccggctc tacctgcagg gccgccatca ttggaaccgc tggaccgaag aaggtttcta
     2821 caaagcaatc ggttactttc tgcaagccgt cgaaaaggat cccggctacg ctctcgcctt
     2881 cgccggggta gccgattctt acgtgctttt gggatggaac agctatctcc ctcccaagga
     2941 cgctttcccc caaggccgag cggctgccat gaacgccctg cagctcgacc ccggtctcgg
     3001 cgaagctcac gcctctctgg cggcggtgtt gtggctctac gattggcagt ggcctgaggc
     3061 tcagagcgaa ttcaagcgca gcctcgcgct caatccgacc tatccaaccg ccaaccattg
     3121 gcatgccgag tttgtgatga ccatgggcct cccgatcgaa gcgatcgcca aaatgaaaaa
     3181 cagccaggag ctggatccgt tgtccctcat catcaacgtc gccatcggat gggccttcta
     3241 catggcccgc cgctacgacg aagccatcga acaacttctg cgcacggtcg aattggatcc
     3301 tcactatcct gtcacttact ggattctg
//
LOCUS       DASTPM010000691         4234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_197875, whole genome shotgun sequence.
ACCESSION   DASTPM010000691 DASTPM010000000
VERSION     DASTPM010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197875"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..522
                     /locus_tag="VFR42_12220"
     CDS             85..522
                     /locus_tag="VFR42_12220"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEU0369974.1"
                     /translation="MFRLDELPTHSAAADDFYAHALVRIQRCMVVLAITVPIFGGLYF
                     GLRTALGLGAGCLVALLNFVWLKRGVEALADRVVGGGKTQPGKGIVTRFLLRYVLMAA
                     IAYGIFTVSPASLYGLLFGLSLPVAAIACEAAYEAYVALARGL"
     gene            631..1362
                     /gene="atpB"
                     /locus_tag="VFR42_12225"
     CDS             631..1362
                     /gene="atpB"
                     /locus_tag="VFR42_12225"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU0369975.1"
                     /translation="MPEQLWFTEILNRLFGAAVTGLLERLGIHPHHPQAPISNSAAME
                     ILVCLFLIIVFFVVRASLSVDRPGALQHAFEGVVGFIEQQSHEIIGPHSEVHTPFLVA
                     LALFILFCNLIGLIPGFESPTAVPIVPLGCALVAFFYYQAQGFKHGGIKYLAHFAGPM
                     PLLAPLMIPIELISHLARVLSLTIRLFANMFAGDMVTLVFFSLVPLGVPIIFLGLHLG
                     VSFLQTYIFVLLTTVYLAGAVAEEH"
     gene            1534..1836
                     /locus_tag="VFR42_12230"
     CDS             1534..1836
                     /locus_tag="VFR42_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369976.1"
                     /translation="MRKLSYVFMILSALCLAVPALAQGGEASAVNWVAITSGFAMAIA
                     SAVCGLAQAKATAAAAEGLARNPAARPGIQLALILGLALIESLALYTLVIIFAKVK"
     gene            complement(2007..>4234)
                     /locus_tag="VFR42_12235"
     CDS             complement(2007..>4234)
                     /locus_tag="VFR42_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU0369977.1"
                     /translation="LGPEFKQWLNDPQKLLQLIAAAEGAKGGGGGEPGGAPIGSVPTV
                     RIGGGGGRGGTGTGSGSGTGTGTGSGSGVWDGKTVPLQEEEVVQAIRLLTRFSEVSVN
                     PNVAPEDIQKELGDADPNTRVNLQQLLESLASNVQTDSDTPLLMKAAEHMAIRFALER
                     YQKGEVKVNAVHQMMEHMSRQMDTLRQILRVQEEKMGKAGILVESHADILDRMFWAEV
                     PEAGKKSVLTSNEAPCVPPRNVRQFVEVLLERGDNESADAILRNYFNCLDAKDAEPRR
                     KIATGLAQLADLYPGPGGTLMACAISRVGEQICREQDTELQGMLSAAFVRLSQEASTR
                     KTYGAVGAVCAAMEQIAKARPVLANDLRPRIGVENRLPEFIEEALRAEQVPEDLLQVL
                     RRTSQAAVEHLADRFFRCMRRDECDRMVELTRELATAALGQMRDMLRTGQPRQAASVV
                     GLLSRLDVLTLLELLPVRLPEFNRFYHDVAVRQIAYGAANDRGRTLLELLEILDPLVV
                     PQAVDEIGMSGDRTAVGPLVAMARAGEAEGRSHFLQLKAIESLGRLRDTEATSVLREL
                     LEAKKMWKWLHHREIRIAAAQALAKIDPRYGSQILSDSGLEPGELAIAALDSAPACPW
                     VRQRRYERIVLAKTLSATISSSWGKSSITIREMSLGGGMGTKEDNLRIGSEANLDISM
                     GVRHVRAQVLLRRARVNEVGFEIVNTDLESRYRLRRILVDAVERAPEGKNQEWDGMRK
                     V"
BASE COUNT          766 a         1342 c         1144 g          982 t
ORIGIN      
        1 gagtgtgagg gataaacata ggccaccgga attggggagc ctggcaggcg cgtgactttc
       61 tagcgggcct cgctgtctga taccgtgttt cgcttggacg aattgccgac tcactcagct
      121 gccgccgacg atttctatgc ccacgcactt gttcgtattc agcggtgcat ggtcgtgctc
      181 gcgataacgg ttccgatttt tggcggcctt tacttcggac tacgcaccgc actgggcctc
      241 ggcgccggat gcctggtggc gctgctcaat ttcgtctggc tgaaacgcgg agtcgaagcg
      301 ctcgccgacc gagttgtcgg cgggggcaag actcagccgg gaaaaggcat cgtgacgcgc
      361 ttcctattgc gctacgtttt gatggccgca attgcctatg gtatctttac tgtttccccc
      421 gcgagcctct acgggttgtt attcggactg tctttgcccg tagcagccat cgcgtgcgaa
      481 gccgcctacg aagcttacgt agccttggcg cgtggtctgt aaattcagcc tctagtcgct
      541 ggtcttcagt cgtcagctag agaaactctg ctgagcgata atgcccccgg ggcacgtgct
      601 ggcgactgaa gactaacgac tgactctttt atgcctgaac aactctggtt tactgagata
      661 ttgaatcgcc tgttcggcgc ggccgttaca ggcttgctgg agaggttagg aattcatccg
      721 caccacccac aggctcccat ttccaactcg gcggcgatgg agatcctggt ctgtctgttc
      781 cttatcatcg tgttcttcgt tgtgcgggcc agcctttccg tcgaccgtcc gggcgcgctg
      841 caacatgctt ttgaaggtgt agttggattc attgagcaac agagccacga gattatcgga
      901 ccgcacagcg aggtccacac tccgtttctg gtcgccctgg ctctcttcat tttgttctgc
      961 aacttgatcg gcctgattcc cggcttcgaa tcgccaaccg ccgttcccat cgtgccttta
     1021 ggctgcgcgt tggtagcctt cttttattac caggcgcaag gattcaaaca tgggggcatt
     1081 aagtacctcg cgcatttcgc aggaccgatg cctttgctcg cccccttgat gatcccaatc
     1141 gaactcatca gccacctcgc acgcgtgctt tccttgacga ttcgtctttt cgccaacatg
     1201 ttcgcgggcg acatggtcac gctagtgttc ttttcgctgg tcccgctcgg tgtaccgatc
     1261 atctttcttg gattgcactt gggcgtttcg tttctgcaga catacatctt tgtactgctc
     1321 acgaccgtat acctagcggg cgcggtcgcg gaagagcact aaagagtttc gagattcaag
     1381 tttcgggttt cgagcggctc cgcagtgggg ggcgatctgg aaactcgaaa cttgaaaccc
     1441 gaaaccggtt tctgagccat cagcgtgagg gcgtctgcac gggatgacgt caaccaccta
     1501 ctggcggcga tttgatacga ggagccacac tcaatgcgta aactcagcta tgttttcatg
     1561 attctgtccg cgctatgcct tgccgttcca gcgctggcac agggaggcga ggcgtccgca
     1621 gtaaactggg tcgccatcac ctccggattc gcaatggcta ttgcgtccgc cgtttgtggt
     1681 ctggcgcaag ccaaagccac cgcagcggcg gccgaaggcc tggcccgcaa cccggccgcc
     1741 cgtcctggaa ttcagttggc gctgattctt ggcttggcgc taatcgaatc tctggcgctc
     1801 tacacgctgg tgatcatctt cgccaaggta aagtagagca gctcttagct ctgcctttgg
     1861 ctgtgttagc ttgaaaatca aaagcctcca tcgttcgcga tggaggcttt tatgtttttg
     1921 acaccgagtg gaaaggtctt ttggactgca attgaaaact gcagctaacg catgagacta
     1981 acagctaagc gctagcacct gcgcttctaa accttccgca tcccgtccca ctcctggttc
     2041 ttgccttccg gagcccgttc gaccgcgtcc accagaatgc gccgcaagcg atagcggctc
     2101 tccaggtcgg tattaacgat ctcaaaaccc acttcgttca cgcgcgcccg gcgtaacaac
     2161 acctgcgcgc gaacatggcg gacacccatc gagatatcca gattcgcttc cgacccgatg
     2221 cgcaggttgt cttctttcgt acccattccg ccgcccaggc tcatttcccg aatggtaatg
     2281 ctcgatttcc cccaggaact gctgatcgtc gccgaaagcg tctttgcaag cacgatgcgc
     2341 tcgtatcgcc gttgccgtac ccatggacac gcaggcgcgg aatcaagagc ggcgattgcc
     2401 agttcgccgg gctctaaacc gctgtcggag agaatctggc ttccataacg aggatcgatc
     2461 ttggccaatg cctgggctgc cgcgatgcga atctcgcgat ggtgcagcca cttccacatt
     2521 tttttggctt ccagcaactc gcgcaacacg ctggtcgcct cggtatcacg aagccgtccc
     2581 agcgactcga tcgctttcaa ctgcaggaaa tgcgaccggc cttctgcttc tccggcgcgg
     2641 gccatcgcca ccagcgggcc taccgctgtc cggtctccgc tcatcccaat ttcatccaca
     2701 gcttgcggaa cgaccagtgg atcgagaatt tccagcaact cgagcagggt gcgtccgcga
     2761 tcgttcgcag caccgtaggc gatctggcgc acggcgacgt catgatagaa ccggttgaac
     2821 tcgggtagcc ggaccggcag caattccagc agcgtcagca catcgagccg gctcagcaag
     2881 ccaaccacgg aggcggcctg cctgggctgc ccggtgcgca gcatgtcccg catctggccc
     2941 agcgccgccg ttgccagctc tcgcgtcagt tccaccatgc gatcgcactc atcgcgccgc
     3001 atgcaacgga agaaacggtc tgccaggtgt tccaccgcag cctgactcgt gcgtcgcaaa
     3061 acttgcagca gatcttcggg aacctgttct gcccgcagcg cttcttcaat aaattccggc
     3121 agccggtttt cgacgccgat tcgaggacgc agatcgttcg cgagcactgg ccgggccttt
     3181 gcgatctgct ccatcgccgc acagactgcg cccacggctc cgtaggtctt ccgggtgctg
     3241 gcttcctggc tcagtcgcac gaaagctgca ctcaacatgc cctgcaactc ggtgtcctgc
     3301 tccctgcaga tctgctcacc cacccggctg attgcgcaag ccatgagggt tccgccaggc
     3361 ccgggataca agtccgcaag ctgtgccaag cccgtcgcga tcttgcgccg cggttcggca
     3421 tccttggcat ccagacaatt gaagtaattg cgcaggattg catccgccga ttcgttgtcg
     3481 ccacgctcca gcaggacttc gacaaactgg cgaacgttgc gcggcggaac acagggcgcc
     3541 tcattggacg tcagaacgct cttcttccca gcttccggca cctcggccca gaacatccgg
     3601 tccaggatgt ccgcgtgcga ttccaccagg atcccggcct tgcccatctt ttcttcctgc
     3661 acccgcagga tctggcgcag ggtgtccatt tgccggctca tatgctccat catctgatgc
     3721 acggcattga ccttcacttc gcccttctgg tagcgctcca gagcgaagcg gatcgccatg
     3781 tgctctgccg ctttcatcag aagtggagtg tcgctgtcgg tctgaacgtt gcttgccagg
     3841 ctctccagca gttgctgcaa attcacccgc gtattcggat ctgcgtcgcc cagctctttt
     3901 tggatgtctt ccggtgcgac gttcggattg actgagactt cgctaaagcg cgtcagcagc
     3961 cggatcgcct gaaccacttc ttcttcctgc agcgggaccg ttttgccgtc ccatacgccg
     4021 ctcccacttc ctgttccagt tcctgtgccg gacccggacc cggtcccggt gccgcctctg
     4081 cctccaccac cgccgatccg cactgtaggg acgctaccaa ttggggcgcc gccaggctcg
     4141 ccccctccac cgcccttggc cccctcagcc gcagcaatca attgaagaag tttctgggga
     4201 tcgttcaacc actgcttgaa ctcaggtccg aggg
//
LOCUS       DASTPM010000692         5206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_688213, whole genome shotgun sequence.
ACCESSION   DASTPM010000692 DASTPM010000000
VERSION     DASTPM010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688213"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFR42_12240"
     CDS             complement(<1..812)
                     /locus_tag="VFR42_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369978.1"
                     /translation="MDIARPEFKKQKRRRQIIGGAVAFVGLAAATFGVSRLKPAAPSV
                     ERSTVWTDTVKRGPMLRQVRGLGSLVPSQEFIRQIPAETDATVVRIRELPGSQVKADT
                     IILEMSNPQVEQAAMDARLQLKATQAALESMRVKLQSDLMNQRAAAATVNSDYTQAKL
                     QADMNKELRDLGLTPEIAYRNSQTRAEELAIRNSLEGERLDINRKAIDSQVAEQLAKV
                     DQMRALADLKQKQLEALKVRAGIEGVLVDLPLQVGQHVLPGTMLAKVVQPNH"
     gene            complement(1017..1499)
                     /locus_tag="VFR42_12245"
     CDS             complement(1017..1499)
                     /locus_tag="VFR42_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0369979.1"
                     /translation="MKGLFLLALVVVPLVAVNSQAPAPIGFEHWTPADLQAASKTLAE
                     KAASDPHHAATKTLSDYPNDLFLLAHREADGQVELHETQADLFVVQSGSATLLVGGTL
                     LDGETVAPHEKRNGTIQGGTRHKLSAGDIVRIPANVPHQLLLDGGREFNYFVAKIKGY
                     "
     gene            complement(1538..2845)
                     /locus_tag="VFR42_12250"
     CDS             complement(1538..2845)
                     /locus_tag="VFR42_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369980.1"
                     /translation="MTTDLQKTSSPPADPRIGNCLTGPEIASAVRETLAATRFIDIHT
                     HLFAHSFGKLSLWGIDELLTYHYLEAELFRSSDVAPEDYWRLSKSERADLIWRTLFVE
                     NTPLSEATRGVIAVLHAFQLPTHQGALGEARVFFKEQKLETHIPRVFRMAGLSSVIMT
                     NDPLDPDEAPLWQKGAEGHEQFHAVLRLDRILNAWPTHWQVLAAQGYAVDAHASGKST
                     SEVRRFLADWATRMKPVYMACSVPDAFEFPSESLNGLLLRDAVLPACREFRLPLSLMI
                     GVRKQVNPRIRLAGDGVGRADLRTVETHCRDFAANRFLVSVLSRENQHELCVLARKFN
                     NLLPFGCWWFLNNTSIVEEITRERIEMLGASFIPQHSDARVLEQVIYKWANTRRTVAN
                     ILAENYRLLSDDGRGVTRSDIQRDVNRLFRSNAESWMNLGPQA"
     gene            3131..4474
                     /locus_tag="VFR42_12255"
     CDS             3131..4474
                     /locus_tag="VFR42_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0369981.1"
                     /translation="MKQKNTVDQPSSNGSAVASVEHSGILRTAGLLADRYRWLICGLL
                     FLGVTKNYMDRQVLGVLKGPLQHQFGWNEVDYGNLVFVFQASYAAGMIAMGRVIDRLG
                     SRAGYAFAVTFWSIASMAHAIASSLATFAIARAALGFGEAGVFPASIKSVAEWFPKKE
                     RALATGIFNSGTSVGAILTPLFAPWVALHWGWRWAFLVTGALGFAWLALWLLLYQRPE
                     QHPRCTAQELAHINSDAVPAAGKIRWAQLAKYKQTWAFAAGKFLTDPIWWFYLFWIPD
                     YLQREHGLRLTQIGLPILAIYLISDLGSVAGGWLSSGLIRAGVSVNAARKWTMLACAI
                     CVLPIAAVYKIAGLWPATLLIGLAAAAHQGFSANLYTLTSDLFPSRAVGSVVGIGGMA
                     GAIGGMLIAQIVGHVLQWTNSYMIPFFIAACAYVMALLVIHALTPRLDPVPIDAV"
     gene            4560..4634
                     /locus_tag="VFR42_12260"
     tRNA            4560..4634
                     /locus_tag="VFR42_12260"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4592..4594,aa:Thr,seq:cgt)
     gene            complement(4891..>5206)
                     /locus_tag="VFR42_12265"
     CDS             complement(4891..>5206)
                     /locus_tag="VFR42_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369982.1"
                     /translation="GNMYTYLFGPRISYRHMGRITPFGQTLFGVAHALNGTYLNGKQT
                     DFAMTVGGGFDFRLSRRISIRPIQADYLLTRFNEFETFQLNTSNSTQNNLRLSTGLVF
                     HF"
BASE COUNT         1065 a         1614 c         1394 g         1133 t
ORIGIN      
        1 aaatgatttg gctgaacaac tttcgccaac atggtcccgg gcagaacgtg ctgcccaacc
       61 tgtaatggca gatccaccag aacgccttcg atgccagcgc gcaccttcaa cgcctccagc
      121 tgtttttgtt tcaggtcagc cagcgctcgc atctggtcga cctttgccag ctgctccgct
      181 acctgtgaat caatcgcttt gcggttgatg tccagccgtt ccccttccag tgagtttcga
      241 atcgccaact cttccgccct ggtctgcgaa ttcctgtagg caatctcggg agtcaagccg
      301 aggtcacgca gctccttgtt catatcagcc tgcaacttcg cctgcgtgta gtcggagttc
      361 acagtagcgg ccgcggctct ctgattcatc aaatcgctct gcagttttac gcgcatgctc
      421 tcgagcgccg cttgcgtcgc cttgagctgg agccgggcat ccatggccgc ctgctccacc
      481 tgcggattgc tcatctccag aatgattgtg tcggccttta cctgcgaacc gggcagttcc
      541 cgaatccgca caaccgttgc gtcggtttcg gcaggaatct gccgaataaa ttcctggctc
      601 ggaaccaacg agccaagacc gcgaacttgc cgcagcatcg ggccccgctt gacggtatcg
      661 gtccaaacag tgcttcgttc gacggacggt gcggcaggct tcagacgcga aactccgaaa
      721 gtcgcggccg ccaggcccac gaacgcaacc gctccaccaa tgatttggcg gcggcgtttc
      781 tgcttcttaa attctggccg tgcaatatcc atgaacattc ctcgtgcaaa tgctcagcca
      841 acagaccagc catgcaagtt gctcagaaat aagaaggtac gaggatcgtg agagaatgtt
      901 tccggggcga gtcgtccggt cgtggacttc gccgtccatt tccggacagt gccctgacac
      961 ggatcggcta gtccatggga caagcctctt ctttcagctc cgggagcaca aactggctaa
     1021 tagcctttga ttttcgcaac gaagtagttg aattcccggc cgccgtccag cagcagctga
     1081 tgcggcacat tcgctggaat ccttacaatg tctccggcgc taagcttgtg ccgtgtgcca
     1141 ccctgaatcg tgccgttgcg tttttcgtgc ggtgcaaccg tctccccgtc cagcagcgtg
     1201 cctcccacaa gcagcgtcgc cgaacccgac tgcaccacga acagatccgc ctgcgtttcg
     1261 tgcagttcca cctggccgtc cgcttctcgg tgcgccagca ggaacaggtc attcggataa
     1321 tccgaaagcg ttttcgtcgc ggcgtgatgc ggatcactgg ccgctttttc cgccagcgtt
     1381 ttgctcgctg cttgcaaatc ggctggcgtc caatgctcaa agccgatagg cgccggtgct
     1441 tgagaattta cggccaccag cggcacaacc accaacgcga gcaagaatag ccctttcatt
     1501 ttccttcacc tcggcgagca aaaatctagc aaagccatca tgcctgcggt cccagattca
     1561 tccacgattc cgcattcgaa cggaacagcc ggttcacgtc ccgctggata tcgctccgcg
     1621 tgacgccgcg cccgtcgtca gacagcagcc gataattttc cgccagaatg ttggccacgg
     1681 tccgccgcgt attagcccac ttatagatga cctgctcgag aacgcgcgca tccgagtgct
     1741 gcggtataaa gctcgcaccc agcatttcga ttcgttcgcg cgtgatctcc tccacgatcg
     1801 acgtgttgtt caggaaccac caacacccga atggcagcaa gttgttgaac tttcgcgcca
     1861 gcacgcacag ctcgtgctga ttctcgcggc tcagtacgct cacgagaaac cggttcgcag
     1921 caaaatcccg acagtgcgtc tctacggtgc gcaggtccgc gcgccccacc ccatcgccgg
     1981 ccagtcgaat acgcggattc acctgcttgc gcacgccgat catcagcgaa agcggcagcc
     2041 ggaattcgcg gcaggcgggt agcactgcgt cgcgcagcaa aagcccgttt aaggattcac
     2101 tcggaaattc aaacgcgtcc ggcacagaac acgccatgta tacgggcttc atacgcgtag
     2161 cccagtccgc cagaaagcgg cgcacttccg aagtagactt gccggaggcg tgcgcatcga
     2221 ccgcatatcc ctgcgcggcg agcacttgcc aatgcgtggg ccacgcattg agtatgcggt
     2281 ccagccgcaa caccgcatgg aattgctcgt gcccctcggc acctttctgc caaagcggcg
     2341 cttcgtccgg atcgagcgga tcattggtca tgatcacgct gctgaggcca gccattcgaa
     2401 aaacacgcgg aatgtgagtt tcgagtttct gctccttgaa aaaaacgcgc gcctcgccca
     2461 gcgcgccctg atgcgtcggc agctgaaacg catgcaacac tgcgatcacc ccacgggtcg
     2521 cctcagagag cggcgtattt tccacaaaca gggttcgcca gataagatcg gcccgctcgg
     2581 atttcgaaag cctccaataa tcctcgggcg ctacgtccga cgaccgaaac agctcggcct
     2641 caagatagtg atatgtaagc agctcgtcga ttccccacag cgacagtttg ccgaacgaat
     2701 gcgcgaacaa atgtgtatgg atgtcgatga aacgcgtcgc cgccagagtt tcgcgcacgg
     2761 cgctcgcgat ttccggcccc gtcaagcaat tcccgattct gggatcagca ggcggagacg
     2821 aagttttctg aaggtccgtg gtcacgcttc tatcctaggc accgcaatct atacatcggc
     2881 aatccactga gccgtaggcg gccgtgctaa gttggttcag gaattcgccg gcgatcgaag
     2941 aaccgctttc ccgaacttga gaaatgcaga gtatgcgggg accgccaaag agtcaaattg
     3001 tgagttcttc tgctcgcgaa gaactaagta ggccgaacca taaatgcggt tatcgtgaga
     3061 cgcttggcag gggcagagtt gaccggccga gaatcataaa gcgaagtccg ccagcgcggg
     3121 taaaattccg atgaagcaga aaaacaccgt cgaccaacct tccagcaacg gtagcgccgt
     3181 tgcttccgtc gagcactccg gcatattgcg aacagccggc ctgctcgccg atcgctatcg
     3241 ctggctcatc tgtggacttc tcttcctcgg cgtcaccaaa aactacatgg atcgccaagt
     3301 cctcggcgtg ctcaagggcc ccttgcagca ccagttcggc tggaacgaag tcgattacgg
     3361 aaatttggtt tttgtttttc aggcatccta tgcggccggc atgattgcca tgggccgagt
     3421 catcgatcgc ctaggctctc gcgcgggata cgcattcgcc gttaccttct ggagcatcgc
     3481 ctccatggcg cacgccatcg ccagttcgct cgcgacgttt gccattgccc gcgccgccct
     3541 tggcttcggt gaagccggcg tctttcccgc cagcatcaaa tccgtggccg agtggtttcc
     3601 caaaaaagag cgcgccctgg ccaccggaat ttttaattca ggcaccagcg ttggagcgat
     3661 cttgaccccg ctatttgcgc cctgggtggc cctacattgg ggttggcggt gggcctttct
     3721 ggtaaccgga gctcttggtt tcgcgtggct cgcattgtgg ctcctgcttt accagcggcc
     3781 agagcagcac ccacgctgta ccgcgcagga gctggcgcac atcaacagcg acgccgtgcc
     3841 cgccgctgga aaaattcgct gggcccaact tgcaaagtac aagcaaacct gggccttcgc
     3901 cgcgggaaaa tttcttaccg atcccatctg gtggttctac cttttctgga ttccggacta
     3961 tctgcagcgc gagcatggct tgcgcctcac gcaaatcggc ctgcctattc tcgcaatcta
     4021 tctaatctcc gacctcggca gcgttgcggg cggctggttg tcctctggct tgatccgagc
     4081 cggcgtctcc gttaatgcgg cgcgcaagtg gacaatgctg gcctgtgcga tctgcgtcct
     4141 gccaattgcc gccgtttaca aaattgccgg cctttggccg gcgacactcc ttattgggct
     4201 ggccgccgcg gcgcaccaag gtttctcggc caatctgtac acgctcacct cggacctctt
     4261 cccctcccgc gccgttggct cggtagtggg catcggcggc atggccggcg ccatcggcgg
     4321 aatgctgatc gcgcaaatcg tcggacacgt cttgcagtgg accaacagct acatgattcc
     4381 ttttttcatc gcggcgtgcg cctacgtgat ggcactcctg gtaattcacg cgctcacgcc
     4441 gcgcctcgac cccgttccca tcgacgcggt ataacctact ttcacaggtt cagcgatgga
     4501 aatcacgtcc gcgcccgaat tgcagagacc tcgcggcgaa tgctatcgta gagaaccacg
     4561 ccgaggtagc tcagtggtag agcagccgat tcgtaatcgg caggtcgcga gttcaactct
     4621 cgccctcggc tccactttct tctgattcct caattagtta gacctgactt gtaattctcg
     4681 gccttcgcgc agactcgccg tttcgccccg aaaccgcccg ttctccgatt tgggcaattg
     4741 gtaagtctcc tgtaacgctg ttatcgggcg ctgaggtgtt cggggtccgc gcattctgcc
     4801 taaacacgca gcccctctgc ctgcaccggg acaaacgacg ccaatttcga cgtttcggcg
     4861 gatagcctca agagccaaga caattcccaa tcagaagtgg aatacgaggc ctgtggatag
     4921 ccgtagatta ttttgtgtag aattgctggt attcaattga aaggtctcga actcattgaa
     4981 acgggtcagt aggtagtccg cttggatggg acgaattgat atgcgtcgag acaaccggaa
     5041 gtcgaatccc cctccaacag tcatcgcgaa atcagtttgc ttgccgttga ggtatgttcc
     5101 attgagggca tgcgctaccc caaaaagcgt ctgcccaaaa ggagtgatac gtcccatatg
     5161 gcgataagaa atgcgcggtc cgaataaata cgtgtacatg ttgccg
//
LOCUS       DASTPM010000693         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1380064, whole genome shotgun sequence.
ACCESSION   DASTPM010000693 DASTPM010000000
VERSION     DASTPM010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380064"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1635
                     /locus_tag="VFR42_12270"
     CDS             400..1635
                     /locus_tag="VFR42_12270"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEU0369983.1"
                     /translation="MMKLQDVILKAMAKKLTWIEAAEIAGMSVRNMQRKRQAYVDYGY
                     NGLFDKRRGKISYHRVPMATAERVLGLYRDKYPDFNVRHFHEKLKESEDISLSYSWVK
                     QALQGAGLVARQKKRGPHRRRRPRRPLPGMLLHIDGSKHRWFQDDRYYDLLVILDDAT
                     SRIYYAQLVEEESTRTVMTALREVIEREGLFCALYSDRASHFFVTPKAGEPVDKSRLT
                     QVGRALKELGVQMIPAYSPQARGRSERSFGTWQSRLPQELRLAGIATIEQANRFLRER
                     YIAEFNHKFTVKAAERGTAFRRCGRSDLDWIFSIQTERVVAKDNTVAIRDQWWQLDKT
                     RWRHTLAGQTVTIHQHLDASVSIRYGPHVVGRGKDGALENPKPVFHRSLEIASGDSHF
                     PTAPTTNPLSPKAKAKRVA"
BASE COUNT          430 a          589 c          605 g          379 t
ORIGIN      
        1 gggaaaaatg gttgggggcg ggctggcctg ctcgatttcg gctggcgtcc cgtgcgcgaa
       61 ccgcgcatgg aggtggatca ctcaacccct tggtcacgtt caaccagttc tccgtccaaa
      121 atcgttcagc ctgcgccgcc ccttatgttg tccggttcta tctcgccacc tcccgtttaa
      181 gccgcggccg gcgcgaccat ctaatctctt taattccctt ctctttccgc ttgcattctc
      241 gctccgatgg gaccgcctgt caatagagtt agtgttccgt gtcgttaaca cgttagttgt
      301 ccggtggaat tagtttgtta gttgtccggt ccaggccggg cgggggaaag tggcgaggtg
      361 gaaccgagcc agatgatgcc cgcccgagaa acggagcgga tgatgaaact acaagacgta
      421 attctgaaag caatggcgaa gaagctgacg tggatcgagg cggccgagat agccggaatg
      481 agcgtgcgca acatgcagcg caagcggcaa gcctacgtgg attacggcta caacgggttg
      541 tttgacaagc gacgaggcaa gatcagctac caccgggtgc cgatggcgac ggcggagcgg
      601 gtgttggggc tgtacaggga caagtacccg gacttcaatg tgcggcattt ccacgaaaaa
      661 ttgaaggaaa gcgaagacat ctcgctgagc tacagctggg tgaagcaagc gttgcagggc
      721 gcgggcctgg tggcgcgcca gaagaaacgg ggaccccatc gccggcgtcg gccgcggcgg
      781 ccgctgccgg gcatgctact ccacatcgac ggcagcaagc accgctggtt tcaggacgat
      841 cggtattacg atctgctggt catcttggac gatgccacca gccggatcta ctacgcgcaa
      901 ctggtggaag aagagtcgac gcgcacggtg atgacggcgc tgcgcgaggt gatcgagcgc
      961 gaaggcctgt tctgcgcgct ctacagcgat cgcgccagcc actttttcgt cacacccaag
     1021 gcgggcgagc cggtggataa atcgcgcctg acgcaagtgg ggcgggcgct gaaggagctg
     1081 ggtgtgcaga tgattccggc ctactcgccg caggcgcgcg ggcgcagtga acgcagtttc
     1141 ggaacctggc agagccgctt gccgcaagaa cttcgcttgg ctgggatcgc cacaatcgaa
     1201 caagccaacc gcttcttgcg cgagcgctac atcgccgagt tcaaccacaa gttcacggtg
     1261 aaggccgccg aacgtggcac tgcctttcgt cgctgcgggc gcagcgatct ggattggatc
     1321 ttctcgattc aaactgaacg cgtggtggcc aaagacaaca ccgtcgccat ccgcgatcaa
     1381 tggtggcaac tcgacaagac gcgttggcgc cacaccctcg cagggcagac cgtgaccatc
     1441 caccaacatc tcgacgccag cgtctcgatt cgttacggtc ctcacgtggt gggccgtgga
     1501 aaagacggag ccctggaaaa cccaaaaccg gttttccacc gctccttgga aatcgcctcc
     1561 ggcgattccc actttcccac cgccccgact accaatcctc tctctcccaa agcaaaggcg
     1621 aagagggtag cctgaaacca aaccggacaa ctcacgtgtt aataaaaccg gacaacttca
     1681 aaagctaacg acacgccgct tgttccgccg tgacgccccg aatagcgccg cgcagattcg
     1741 gcccgtgcca cggcttctta tcgaacgcct cgtcgagcgc tgtcaaaagc agcgattttt
     1801 ctgagctcat aacaagtgtg cgggcgtcgg ccgcggtttc agttctggct cagaacgcag
     1861 gtatacgttc acttcttcga gcgatccaaa aaatcccgcg aatgcgtggt tcagatgcgc
     1921 tggacgccgc cgcgattggc ggaagtcgcg cgctcggaat tggtccgtga aagaaatgac
     1981 agcggtgccc agaggccggt att
//
LOCUS       DASTPM010000694         6594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_140172, whole genome shotgun sequence.
ACCESSION   DASTPM010000694 DASTPM010000000
VERSION     DASTPM010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140172"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VFR42_12275"
     CDS             complement(<1..1329)
                     /locus_tag="VFR42_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0369984.1"
                     /translation="MFVDFFIKRPVFATVCAMVIVLAGAISIPTLPVAELPDLAPPQV
                     IVTSTYIGANAQAVESAVTIPLEQAINGVEGMKYITSSSSNAGVSQINVVFDVARPID
                     LAAVDVQNRVNQTLARLPNEVKNTGVIITKQTSGFVFGGGVYSENGQYDSLFLSNYID
                     VYIKDALKRVSGVGDVIIFGERKYAMRLWLDPGKLAQRGLTASTVVTALQEQNVQVAA
                     GQIGQPPSPTGQSYQVSVRAVGRLSEASEFDNIVIKTGSDGTLVRVKDVGHSELGAED
                     YGSLLRFNGHDAVGLGLTQLPGANALDVDRLAKAELVRLSKNFPPGLKVAVAFDTTTV
                     IGESIRDVLQTLIEAIILVIIVIYVFLQDWRSTFIPAVTIPVSLIGTFIFVKAFGFSI
                     NTLTLFGITLATGLVVDDAIVVIENVERHITEGITEPHNAAFVAMQEVAGA"
     gene            complement(1391..2485)
                     /locus_tag="VFR42_12280"
     CDS             complement(1391..2485)
                     /locus_tag="VFR42_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0369985.1"
                     /translation="MASGCGQKTAADNPAGAGAAMPVQVQAVKSVTIPDTTEYLSVLK
                     SRHSANINPQVEGQVIRIFVKSGDRVKAGQALLQIDPLKQEATVSSQEASRAAQEANV
                     ALAKVSFERSKRLFEAGVIAKAEFDSAQSNYDASVAQLKALEEQVKSQRAELKYYSVA
                     APMDGIVGDIPVRMGDRVAVTTLLTTVDEPGALEAYIYMPVDRSRSLRLGLPVKLLDP
                     SGALLANTTITFVSPQVESETQTVLAKAAAPNSQSKLRVSQQVRAQVTWGQREGATIP
                     FLSINRINGQFFAFVSTNEGKGTVARQKLVKIGDNVGNDVEVLDGIKAGEHLIVSGTQ
                     FLQDGTPVTEQSAPAANAQAAQSNSKESSN"
     gene            complement(2896..3999)
                     /locus_tag="VFR42_12285"
     CDS             complement(2896..3999)
                     /locus_tag="VFR42_12285"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEU0369986.1"
                     /translation="MPYRICRVLLFVAILLSGALAAVTTFAQEPVLPPKFGAWTASGT
                     IPSASSLPQPLQQELGAKAGEAADYTSGSKTIHALLETFKDPSSAYAAYTGQLQVGMA
                     PTDVRPYSAVNGERMSLLIGNFLLYVDGVKGISAADLRTLALAVQGHADATPLPPIRN
                     YLPEERLIQGTQRYAFGPAGFQAALGTLSQGKFASLASSIGFSSGAEAMLASYLIAPG
                     KSQSLLLIEYPTPQLAEQHLRHIQADLSGNSELSSTNVERKASLLSLVLSPANERVAA
                     QLRNEINYETQVTWNEPSHTLTDPPWLLVVKNIFLGTFAFCGLALVLGLAFGGVRVIT
                     KRLFPGKVFDRPENMEVLQLGLSGKRIDPRDFY"
     gene            complement(4034..6265)
                     /locus_tag="VFR42_12290"
     CDS             complement(4034..6265)
                     /locus_tag="VFR42_12290"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0369987.1"
                     /translation="MSASSRVSGGILFGFFFNRGFARLSPVFAVAALSAALAAAVASA
                     AFASDGSGHSSETASAEPGAAERRQAASSQFAHAEEQRAALNNKPPDKRTLNEYKQVV
                     SSYRRVYLITPHAAEVPDSLLAVAELYTEMGDRFGRAYYQSAVDSYQFLMREYPTSRY
                     LQDAYLRSAKLQKDQLGDTAGASKTYDAFLKKFPHSKQRREAQEARAELALLQRSAPP
                     DAKQNAIARSTGSDSARETRPPVISRDVAKDFPKDAPRSAAKEAAPEEAGSADDDQPV
                     AAGGKIPQIRRISAQAAADSTRVTIEIESSVQYVSGRVANPDRIYFDLHAARLAQPLL
                     HKKIEADGPLVAAIRTSQSANGVVRVVLEVNGVIDYSASLLNSKKDGASQLVIDLYAD
                     VKPVETRTAEAKVPAKDTASGAANPPPKNAIADKAQPAAASKSAKADTTDAPAAVPIP
                     EPKRTLASAKSGKKPDLVQPASAPQPTRDGQSTLTRALGLKIGRIVIDAGHGGHDTGT
                     IGPTGLMEKDLCLDVALRLGKIIQQRLPSADVVFTRSDDTFIPLEERTHIANEAKADM
                     FVSIHANSSQDTAARGIETYYLNLKGSPEAMEVAARENATSDQGIHELEDLVKQIART
                     EKIDESREFAEDVQDSLSKRVQKTAKVVKNRGVRKAPFVVLIGADMPSILTEISFLSN
                     PADERLLKQPEHRQRVAEGIFQGIAGYLQSLNSMTVNQSNSPVARKTTTSASAGEASR
                     NRN"
     gene            complement(6396..>6594)
                     /locus_tag="VFR42_12295"
     CDS             complement(6396..>6594)
                     /locus_tag="VFR42_12295"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0369988.1"
                     /translation="AYNAGEGAVDRAHGVPRYRETRNYVQRVQSAYFRPGSGRLDGAF
                     INPHAIRKSTDPAGRTVFTND"
BASE COUNT         1291 a         2099 c         1793 g         1411 t
ORIGIN      
        1 cgctcccgcc acttcctgca tcgccacgaa agccgcgttg tgtggctcgg tgatcccttc
       61 cgtgatgtgc cgctcgacgt tttcgatcac tacgatggca tcgtccacca ccaggccggt
      121 ggctagagtg atgccaaaca acgtcagcgt gttgatggaa aatccaaacg ccttcacgaa
      181 tatgaacgta ccaatcagcg agaccggaat ggtgaccgcc ggaatgaaag tacttcgcca
      241 gtcttgcagg aaaacataaa tgacgatgat gaccaggatg atggcttcaa tcagcgtctg
      301 cagcacgtcg cgaatcgact cgccgattac cgtagtcgta tcgaatgcca ctgccacttt
      361 tagtcccgga gggaaattct tagacagccg caccagctcg gccttggcca aacggtcgac
      421 gtctaacgcg tttgcgcccg gaagctgcgt caggcccagg ccgacggcat catggccatt
      481 gaaccgcagc agcgatccgt aatcctccgc gcccagttcc gaatggccga catccttgac
      541 acgcaccagg gttccgtccg agccggtctt gatgacgatg ttgtcgaact cagaggcttc
      601 agaaagccgg ccaaccgcac ggacgctgac ctgatacgac tggccagtcg gggacggcgg
      661 ctggccgatc tgccccgccg cgacttgcac gttctgctcc tgcaatgcgg tgacgactgt
      721 cgacgctgtt aagccgcgct gcgccagctt gccaggatca agccacaagc gcatcgcgta
      781 cttgcgttcg ccaaaaataa tcacgtcgcc gacgccagag acacgcttca gcgcgtcttt
      841 aatgtagaca tcgatgtaat tgctgaggaa caggctgtcg tactgcccgt tttccgaata
      901 cacgccgccg ccaaacacga atccgctggt ctgcttggtg atgattaccc cggtgttctt
      961 cacttcgttc ggcaaacgcg cgagggtttg attgacgcgg ttctgcacgt ccacagcagc
     1021 caaatcaatc ggtcgcgcca cgtcgaacac cacgttgatc tggctgaccc cggcgttgct
     1081 gctcgaagac gtgatgtact tcatcccttc cacgccgttg atcgcctgct ccagcggaat
     1141 cgttacggcc gattccactg cctgcgcgtt cgcgccgata tacgtgctgg taacaattac
     1201 ctgcggcggc gccaggtccg gcagttccgc aacggggagt gtaggaattg aaatcgcgcc
     1261 cgccagcacg atcaccatgg cacagacggt cgcgaacact ggccgcttga tgaagaaatc
     1321 aacgaacact gagaggctcc gtcacaatta gtctgtagcg cctgggtctc atggctgttt
     1381 tttttggagt ctagttcgac gactctttcg aattggactg ggctgcttgc gcgttcgccg
     1441 cgggcgcaga ctgttccgtc accggcgtgc cgtcctgcaa aaactgtgtg cccgacacaa
     1501 tcaagtgttc gccggctttg atgccgtcca gcacttccac gtcgttaccc acgttgtcgc
     1561 cgatcttcac aagtttttgc cgcgccactg tgcccttacc ttcgttcgtc gaaacgaagg
     1621 caaaaaattg cccgttgatg cggttgatgc tgaggaaggg aatcgtcgcg ccttcgcgtt
     1681 gtccccaggt gacctgcgcg cgcacttgct gggaaactcg caacttgctc tgcgaattcg
     1741 gcgcagcggc cttggccagc acggtctgcg tttctgattc cacctgcggc gatacgaacg
     1801 taatcgtcgt gttcgccaat aatgctccgc tggggtcgag caatttcacc ggcagtccca
     1861 gtcgcagcga gcgtgaacgg tccaccggca tgtaaatgta cgcttcgagc gcgcccggtt
     1921 catccacggt cgtcagcagt gtcgtcaccg ccacgcggtc gcccatgcgc actggaatgt
     1981 cgcccacaat accgtccatc ggtgctgcta cggagtaata cttcaattcc gcgcgctgcg
     2041 atttcacctg ctcttccaag gcttttagct gcgccaccga ggcatcgtag ttcgattgcg
     2101 cagaatcgaa ctccgctttc gcaattacgc cagcctcgaa gagtctcttc gaccgttcaa
     2161 aggagacttt tgccaatgcg acattggctt cctgtgcggc ccgggatgct tcttggctgc
     2221 ttaccgtggc ctcctgcttc aaaggatcga tctgcaacag cgcttggccg gcctttaccc
     2281 gatcgcccga cttcacaaaa atccggatga cctgcccttc cacctgcggg ttgatgttcg
     2341 ccgaatgccg cgacttcagc acggacaggt attccgtcgt gtccggaatc gtaaccgact
     2401 tcacggcctg cacctgcacg ggcatggcgg cgccagcacc cgccggattg tcggccgcgg
     2461 tcttctgtcc gcagcccgag gccacgagca aagctcccgc caggctcacg cctagccagg
     2521 ctgcgcgacc acaccgcctg ccgcttctag cgacacttcg caattcgcat gacaactgga
     2581 atggaacgaa catgcttccc cgagtctccg taagaatccc tccagaatgc taggacattg
     2641 gtcgaacgcg gcaaagaact acgccgttcg cggtctctca ttagacgcag cgcaaactct
     2701 gccgttacat ggttttaccg acttccagcc tgctgggtcg cggctagcca tcaaccgccg
     2761 ccacctataa gacgttcgaa ctgcccccga atcgacacgc caaccccgcc aattttgtga
     2821 acaattgtga attttgcgag caagagaagc tcggggagcg ccactaaccg gcaatcgagc
     2881 ctgattcgcg gggtttcagt agaaatcgcg agggtcgatc cgcttgccgg aaagccccaa
     2941 ctgcaacact tccatgtttt ccggccggtc aaacactttg ccgggaaaga gccgcttggt
     3001 aatgacgcgc acgccaccga aggccagccc cagcaccagc gccagaccgc aaaacgcgaa
     3061 cgttcccaga aaaatgttct tcactaccag cagccacggc ggatcggtca gcgtgtgcga
     3121 aggctcgttc caggtcacct gcgtttcgta attgatctcg ttgcgcaatt gcgccgctac
     3181 tcgctcattc gccggcgaca gcaccagcga cagcaaggaa gccttccgct ccacgttcgt
     3241 gctcgacaat tcagaattgc cggaaaggtc cgcctggatg tgccgcaaat gctgctcggc
     3301 cagctgtggt gtcggatatt caatcagcag caagctctgg gatttgccgg gagcgatcag
     3361 ataactggcc agcatcgctt ccgctccgct cgaaaagcca atactgcttg cgagcgaagc
     3421 gaacttgcct tgcgatagtg tgccgagcgc ggcctgaaat cccgctggac cgaacgcgta
     3481 ccgctgtgtc ccctgaatca gccgctcctc cggcaaataa tttcgaatcg gcggcagcgg
     3541 cgtggcgtcc gcatgtccct gaaccgccaa cgccaatgtc cgcaagtccg ccgctgaaat
     3601 ccccttcacg ccgtcgacgt acagcagaaa attcccaatc agcagcgaca ttcgctcgcc
     3661 atttaccgcc gagtacggac gcacgtcggt cggcgccatt cccacctgca gttgcccggt
     3721 atacgcggcg taagcgctgc tggggtcctt gaacgtctcg agcaatgcgt ggatggtctt
     3781 gctgccgctc gtgtaatccg ccgcctcccc cgcctttgct cccagctcct gctgcaatgg
     3841 ctgcggtagg gaactcgcgg aggggatcgt tcctgacgcg gtccacgcgc cgaacttggg
     3901 cggcaacacg ggctcttggg caaacgtggt cacagccgca agcgcgcccg ataggaggat
     3961 cgcgacaaac agcaacacac ggcaaatgcg gtaaggcatt ctcatccctt tggttctatt
     4021 agacaccaga acgtcagttt cggttgcgag aagcttcccc ggccgaggca gacgtggtgg
     4081 tcttgcgcgc aacaggcgaa ttgctctggt ttacggtcat gctgttcagg ctttgcagat
     4141 agccggcgat gccctggaaa atgccttcgg cgacacgctg gcggtgctcc ggctgcttca
     4201 gcagcctttc atctgccgga ttgctcagga acgatatctc ggtcaggatc gacggcatat
     4261 ccgcgccaat cagcaccacg aacggcgcct tgcgcacgcc gcggtttttt accaccttcg
     4321 cggtcttctg cacgcgcttt gagagcgaat cctgtacgtc ttccgcaaac tcccgcgact
     4381 catcaatctt ttccgtgcgc gcaatctgct tcaccaggtc ttccagttcg tgaattccct
     4441 gatccgacgt cgcgttttcg cgcgccgcca cttccatcgc ttccggtgag cctttcaaat
     4501 tcaagtaata cgtttcgatc ccgcgtgccg ccgtatcctg gctggaattg gcatgaatcg
     4561 aaacaaacat atcggctttg gcttcgttgg cgatgtgcgt tcgctcttcc agcgggataa
     4621 acgtatcatc cgagcgcgtg aacaccacgt cggcgctggg caaccgctgt tgaattattt
     4681 ttcccaggcg cagtgccacg tccaggcaca ggtctttttc catcaggcct gtcggcccaa
     4741 tcgttccggt atcgtgtccg ccatgcccgg cgtcaatcac gatacggcca atcttcaatc
     4801 ccagcgcgcg cgttagtgtg gattgcccgt cgcgcgttgg ctgcggcgcc gacgccggct
     4861 gcaccagatc gggtttcttc ccgctcttcg ctgaagccag cgtgcgctta ggctctggga
     4921 tcggcacagc cgcaggagca tcggtggtat cggcctttgc ggacttgctc gcggcagcag
     4981 gctgggcctt atccgcaatc gcgttttttg gcggcgggtt cgctgcgccg gacgccgtat
     5041 cctttgctgg aaccttcgct tcagcggtcc gcgtctcgac cggctttaca tctgcgtaca
     5101 aatcaatcac cagctgagaa gccccatctt tcttcgaatt caacagggaa gcgctgtaat
     5161 cgatcacccc attcacttcc aggacgacgc gaaccacgcc attggcgctt tgtgaggtac
     5221 gaattgctgc gaccagcggg ccgtccgcct caattttctt gtgcaaaaga ggttgcgcca
     5281 gtcttgctgc gtgtaaatca aaatagattc gatcggggtt cgcgacgcgc cccgagacat
     5341 attgcacgct gctctcgatt tcaatcgtca cgcgcgtgga gtccgccgcc gcctgggcgc
     5401 tgattcgccg aatttgcggt attttgccgc ccgccgcaac cggctgatcg tcgtccgcgc
     5461 ttccagcttc ttccggagca gcttcttttg ccgccgaacg cggcgcgtcc ttaggaaaat
     5521 ctttcgcgac gtcgcgagaa attacaggag gccgggtttc tctggcggaa tccgagcctg
     5581 tgcttcttgc gatggcattc tgcttcgcat cgggcggagc ggaacgctgc agcagcgcca
     5641 attccgcgcg cgcttcctgc gcttctcgcc gctgcttcga gtgcgggaat ttcttcagaa
     5701 acgcatcgta cgttttgctt gcgccggcgg tgtcgccaag ctgatctttc tgcaacttcg
     5761 ccgaacgcag atacgcatcc tgcaaatacc ggctggtcgg atactcgcgc atcaagaact
     5821 gataactgtc caccgccgac tggtaatacg cgcggccaaa gcggtcgccc atttccgtat
     5881 acagctcagc gaccgcgagc agggaatccg gaacttccgc ggcgtgcggc gtgatcagat
     5941 atacgcggcg atagctgctc accacctgtt tatattcgtt tagggtgcgc ttgtccggcg
     6001 gtttgttgtt caacgccgcg cgttgctctt ctgcatgcgc gaactgcgaa ctcgccgctt
     6061 gccggcgctc cgcagcgccc ggctccgccg acgccgtttc tgaggaatgt cccgaaccat
     6121 cggaagcgaa agccgcgctc gcaacagcag ccgccaatgc cgcgcttaaa gcggctacag
     6181 caaaaaccgg cgaaaggcgg gcgaagcccc tgttgaaaaa aaagccgaac aaaatgccgc
     6241 cagacacacg gctgctggca gacacaccgc ctcttgcatg ttcagcgtga atcccggact
     6301 tcatccccca acccattgcc caaacggatc ttaaccgctc agctcgctca accattcgca
     6361 aaaaacgcgg tctgtagcgc gccgcatact gtacgctagt cgttcgtgaa aactgtgcgg
     6421 cctgcggggt cggtggactt gcggattgcg tgaggattga tgaacgcgcc atccagacgt
     6481 cccgatcccg gccgaaagta tgcactctgc acccgttgca cgtaattccg tgtttcacgg
     6541 tatctgggga cgccgtgagc acgatccaca gccccctctc ccgcattgta cgcc
//
LOCUS       DASTPM010000695         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_544217, whole genome shotgun sequence.
ACCESSION   DASTPM010000695 DASTPM010000000
VERSION     DASTPM010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544217"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..882)
                     /locus_tag="VFR42_12300"
     CDS             complement(37..882)
                     /locus_tag="VFR42_12300"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0369989.1"
                     /translation="MSPEKLTGAEFKKQLRAGQPKMGLFLNSHSPTIAEQLAHSGYDW
                     LLVDTQHGPMGFEKLSAMLSGIASGGAKSLVRVEGYHDGGGIQQALDLGADGVLIPYI
                     NTAAEARQAVSCARYPTAGTRSVYFPQRSMNKDGLLGYAGNWNNNGILALQVETADCI
                     KNIAEIAAVPGVDILFLGQNDLCMSMGLYEKYEFPHMYTSPELNAATQSLVEHARKNS
                     VILGLFLFGTSRVGEFLDKGFSFVSIGNDLHHVLTQAGAHVGDLERIAKEKGRPWARR
                     STALF"
     gene            1067..1738
                     /locus_tag="VFR42_12305"
     CDS             1067..1738
                     /locus_tag="VFR42_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369990.1"
                     /translation="MRNTDEKLGKPLRLALIGMSGAGKTFWTRRVAESGVQAISCDDV
                     IEQRLAPRLAQGGFSGINGVAAWMGWPDSATYAEREAEYLKEEIATLDEILSGLEKDA
                     ERPLVLDTTGSVIHTGNHLLMRLRKLMKVVHLASTDAEQQLLIERYLSDPKPVLWRGA
                     FAAKAGEIPRHTVARCYPHLIAARRQSYVALAHAQLQVAELRELPPTAEAFFEKVEAA
                     MSRQR"
     gene            1735..3036
                     /gene="thrC"
                     /locus_tag="VFR42_12310"
     CDS             1735..3036
                     /gene="thrC"
                     /locus_tag="VFR42_12310"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU0369991.1"
                     /translation="MRYRSTAGKAALTSLRGAVLRGLAPDGGLYMPAEIARRSPEELE
                     EFRALPFTEVCFRVVKPFATPDVTEDVLWQIVSEAMNFPVQLVSLSPGLHVLELFHGP
                     TLAFKDFGARFMARLMGHFVRGEDRTLTVLVATSGDTGSAVAHGFLGVPGIRVVILYP
                     SKRISEAQEKQFTTLGENITALEVSGTFDDCQRLVKQAFADEALNKRAFLTSANSINI
                     GRLLPQMFYHVAAYRQLPVASTPLVVAVPSGNFGNLTAGLFAKRIGLPVAKFVAATNA
                     NDVVPQYLLSGKYEPRAAQATCSNAMDVGNPNNFPRLLDLCRGRLEYVRREIWGHGAS
                     DAETLAEMKAVWDRYRYIADPHTAVGILGWEAYKKEHAEPAQGLVLATAHAAKFAEIV
                     EQAIGIAPPLPDRLAACLKRPKLSVAMSSSYEDFKQFLQAR"
BASE COUNT          620 a          740 c         1083 g          641 t
ORIGIN      
        1 gcggtgtcgc gaaaggggcg ataattttcg cgacgatcaa aacagggcgg tggaacggcg
       61 agcccaaggc ctgccttttt ctttggctat tctttcaagg tcgccgacgt gggctccggc
      121 ttgcgtcaga acgtgatgca aatcgttgcc gatgctgacg aaggagaagc ctttgtcgag
      181 aaattcgccg acgcgcgagg tgccgaagag gaagaggccg aggatgacac tgtttttgcg
      241 ggcgtgttcg acgaggcttt gcgtggcggc gttgagttcg ggtgaagtgt acatgtgggg
      301 gaattcatat ttttcgtaga ggcccatgga catgcacaga tcgttctggc cgaggaacag
      361 aatatcgacg cctggcactg cggcgatttc ggcaatgttt ttaatgcagt cggcggtttc
      421 gacttgcagg gctaggatgc cgttgttgtt ccagttgccg gcgtagccga gcagaccgtc
      481 tttgttcatg ctccgctgcg ggaaataaac ggaacgcgtg ccggcggtgg gataccttgc
      541 gcagctaacg gcttggcgtg cttcggcggc ggtgttgatg tagggaatca gaaccccgtc
      601 ggctcccaga tcgagcgcct gctgaatgcc gccgccgtcg tggtagccct cgacgcgcac
      661 cagcgatttg gcgccaccac tggcaattcc cgacaacatg gcggaaagct tttcgaagcc
      721 catggggccg tgctgggtgt cgacgagcag ccagtcatag cccgagtgag cgagctgttc
      781 ggcgatggtg gggctatgcg aattgagaaa gaggcccatc ttgggctgtc cggcgcgaag
      841 ctgctttttg aattcggcgc cggtgagttt ttctggggac atgattcgct cctcgagctg
      901 aaatacttca gaagaatttt ggatccggtg acctaacaca gtacacgtgg aagaggcatt
      961 ttccaaacgc ccactcgatg tggcgggaac ctatcagcat ttctaatgca ggcgggtgaa
     1021 aaacggcgct tataattatg ggcagttcgg gagtgtcctg cgattcatgc gcaatactga
     1081 cgagaaactc ggcaagccgt tacggctagc gttgattggc atgtctggcg cggggaaaac
     1141 attctggacg cggcgggtgg cggagagcgg agtgcaggcg atttcgtgcg acgacgtgat
     1201 tgaacaaaga ttggcgccgc gactggcaca gggtggattt tccggaatta acggcgtggc
     1261 ggcgtggatg ggctggccgg attcagcgac gtatgcggaa agggaagcgg agtatttgaa
     1321 ggaagagatc gcgacgctgg acgaaatttt gagtgggctg gaaaaggatg cggagaggcc
     1381 gctggtgctg gatacgacgg gaagcgtgat tcataccggg aatcatctgc tgatgcggct
     1441 gcggaaattg atgaaggtgg tgcacctggc gtcgaccgac gcggagcagc agttgttgat
     1501 cgaaagatat ttgagcgatc caaaaccggt gctgtggcgc ggagcatttg cggcgaaggc
     1561 gggcgaaata ccgcgccata cggtggcgcg gtgctatccg catttgattg cggcgcggcg
     1621 gcaaagttac gtggcgctgg cccatgcgca gttgcaagtg gcggagctgc gggagctgcc
     1681 gccgacagcg gaagcgtttt tcgagaaggt ggaagcagcc atgagccgcc agcgatgaga
     1741 taccggagca cagccggcaa ggcggccctg acctctctgc gcggagcggt gttgcgtggg
     1801 ctggcgcccg atggcggctt gtacatgccg gcggaaattg cgcggcgttc gccggaagag
     1861 ctggaagaat ttcgcgcgct gcccttcacc gaggtttgct ttcgcgtggt gaagccgttt
     1921 gctacgccgg acgtgacgga agacgtgctg tggcaaattg tgagtgaggc gatgaacttt
     1981 ccggtgcagc tggtttcgct gtcgccgggg ctgcacgtcc tggaactatt tcacggtccg
     2041 acgctggcgt tcaaggattt tggcgcgcgc ttcatggcgc ggctgatggg ccattttgta
     2101 cgcggcgaag atcgcacgtt aaccgtgctg gtggcgactt cgggcgatac gggcagcgcg
     2161 gtggcgcacg gatttctggg tgtaccgggt atccgcgtgg tgattctgta tccgtcaaag
     2221 cgaatcagcg aggcgcagga gaagcagttc accacgctgg gcgagaacat cactgcgcta
     2281 gaagtaagcg gaacctttga cgattgccag cggctcgtga aacaagcatt cgcggacgaa
     2341 gcgctgaaca agcgcgcgtt tttgacctcg gcgaattcga tcaatatcgg gcggctgctg
     2401 ccgcagatgt tttatcacgt ggcggcgtac cggcagttgc cggtcgcttc gacgccgctg
     2461 gtggtggcgg tgcccagcgg gaatttcggg aatttgacgg cggggctgtt tgcaaagcgg
     2521 attggattgc ccgtggcaaa gtttgttgcg gcgaccaacg cgaacgacgt ggtgccgcaa
     2581 tatttgctga gcgggaagta cgagccgcgg gcggcgcagg cgacgtgttc gaatgcgatg
     2641 gacgtgggga atccaaacaa ttttccgcgg ctgttggatt tgtgccgcgg gcgactggag
     2701 tatgtgcgac gggaaatttg gggacacggc gccagcgacg cggaaacttt ggcggagatg
     2761 aaagcggtgt gggacagata ccgctacatt gccgacccgc acacggcggt ggggatttta
     2821 ggttgggaag cgtacaagaa ggaacatgcg gaaccggcgc agggattggt gctggctacg
     2881 gcgcatgcag cgaagttcgc tgaaattgtg gagcaggcga ttggaatcgc gccgccgctg
     2941 ccggaccggc tggcggcgtg tttgaagcgg ccgaagttgt cagtagcgat gtcgagttcg
     3001 tatgaggatt tcaagcagtt cttacaggcg cgctagccca cccacgcaaa agaaatcagc
     3061 ttcctcgcta cgctcggaat gaca
//
LOCUS       DASTPM010000696         6867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_111441, whole genome shotgun sequence.
ACCESSION   DASTPM010000696 DASTPM010000000
VERSION     DASTPM010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111441"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFR42_12315"
     CDS             <1..408
                     /locus_tag="VFR42_12315"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0369992.1"
                     /translation="RYFFLYVVTGGFGFLASAFLGHYSLGASGALLGLVGAMLAVTTK
                     RGGAFMRDLRARLISSVVILFMLGLAGMGLDNTAHFAGLVAGFALGKIYADRQPMNVS
                     ELRRAYAMGWLAGVVVVASFVLMLLHYRDPISG"
     gene            502..810
                     /locus_tag="VFR42_12320"
     CDS             502..810
                     /locus_tag="VFR42_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369993.1"
                     /translation="MEPHSIVVVSLHTPKEKFWGELLAISTAGITLRGIDLNSFDHFV
                     NQINGAEGERTGLPTLFFPMTRVERIALDEPSGSIPSLSEVFARKVGRPLAEYLAQFA
                     "
     gene            927..1475
                     /locus_tag="VFR42_12325"
     CDS             927..1475
                     /locus_tag="VFR42_12325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0369994.1"
                     /translation="MKGRFWTVPNQITLLRLGILPFFLILIAYEHYRYALLILVLAGC
                     SDGIDGLLARKLNQKSAIGAYLDPIADKLLLSSSFILLAMEKQLAWWLTIMVLSRDIM
                     MLTVAAVIILISGYRPFPPSLLGKATTLFQIILVFVVVLQAGYPDLGLTNVVRFLMYF
                     VAVITTVSGFHYSFAIARRLSS"
     gene            complement(1538..2206)
                     /locus_tag="VFR42_12330"
     CDS             complement(1538..2206)
                     /locus_tag="VFR42_12330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEU0369995.1"
                     /translation="MMTEEQKRERIVKRIARELRDGFYVNLGIGIPTLVANHVPPGME
                     VILQSENGMLGVGPYPLESEVDPDLINAGKETISELKGCCYFSSADSFAMIRGGHIDL
                     TVLGALQVDEKGTLANWAVPGKMLKGMGGAMDLVAGAKRVFVAMEHATKDGKPKILTK
                     CSLPLTGVEVVDHIVSELAVIEVTAEGLVLRELAPDTTVEQVQALTEPKLILPPGGPA
                     AMAG"
     gene            complement(2203..2613)
                     /locus_tag="VFR42_12335"
     CDS             complement(2203..2613)
                     /locus_tag="VFR42_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0369996.1"
                     /translation="MHVKGATNQTDTFAANAKSNSRLPLPLAVVFCLYLFFVGTAFIK
                     VVPVFSNLFAGLQVEILWPTRLLLLSYWWALPFFSFMAIALAVVRQIIPLNRAQRRAM
                     HAYLFFAAVVLPSLVVLALYMPTLKLMWKAHSLG"
     gene            complement(2649..3344)
                     /locus_tag="VFR42_12340"
     CDS             complement(2649..3344)
                     /locus_tag="VFR42_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEU0369997.1"
                     /translation="MDKCISSADEAVANLQDGMTILMGGFGLCGIPENLIAAVQRKGT
                     KNLTVVSNNAGVDGFGIGILLEKRQVKKMVSTYVGENKLFEQLVTSGELELELNPQGT
                     FAERIRAGGAGIPAFYTPTGHGTLVAEGKETREFEGRQYVMERGIRGDFAFIKAWKGD
                     RWGNLIYRKTARNFNPMMATAADYVIAEVEELVELGSLDSDSVHTPGLYVNAIFQGPK
                     YEKRIERRTTRKV"
     gene            complement(3351..4352)
                     /locus_tag="VFR42_12345"
     CDS             complement(3351..4352)
                     /locus_tag="VFR42_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU0369998.1"
                     /translation="MSPHLIQRVTVLGTGLIGGSFALALKKYTTDIFVRGWDRASVVN
                     EAHGRRIIDQSSSGSVKEALDGADLIYIALPIGATLDLLPEIARFASPNALITDACST
                     KLRITQDAAALFPGGAGTLFLGGHPMAGKELSGIAAADSDLFRDHPYALITEPSSDAA
                     LIATAAEAAAVADAASAQSAIAMPPGIPEEPLLSRQRHSPESDPRVGAFVKILEKIGA
                     RPMWIGAQQHDYAVGLASHLPQLAAVALASFLYDRLDENGLPITLAGPGLRDSLRLAG
                     SSYTLWRDIVLTNREILSASLDLFARRIDELRERLGSRDLEGDFDAANELYKLLKGI"
     gene            complement(4439..6334)
                     /locus_tag="VFR42_12350"
     CDS             complement(4439..6334)
                     /locus_tag="VFR42_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0369999.1"
                     /translation="MADAFQIGPWVAVPSLNTLSHNGKNVRIEPKVMEVLVCLAGRPG
                     ETISKEKLLEVVWPGTFVTDDVLTRCVSELRRVFEDDPRQSRFIQTIPKRGYRLIAPI
                     APVTAGGPSIRVGPSEGSYSAVNSAKAPSAIRKAVVMATATVVFAALLLAVDVKNVRG
                     HLSKLSGTPKISSLAVLPLTSLSADPNQEYFSDGLTDALTTELSQISSVRVISRTSAE
                     RYRNTNKPLSQVARELGVNGIVEGTVQKSGDSIRVTAQLIYAPDDRHLWARSYERDSQ
                     NVLALEQDIARAVAEEIRATLTPREKARLARSKPLSVVALELFLQGRYHSERAAAVTY
                     RNGQRAVRQRELADARGFFEKAIQEDPAYAQAYVGLAQTWQDEPVSDDGPEKAEALLR
                     KGIALDPELAEAHEALATLDVLRLWRWSEAEQEYRRAIQLNPNSAEAHARYAEYLDCM
                     RRFDEGMKEFLLSQSLDPGHDFNPNPFYRRREYDRAIEEDEGEVKRQAFGFWSHWNLA
                     YDYEGAGRRQDAAREWEQAIRMLGYTELADSMHRGLERGGYNGAITALVAGMEAADAR
                     GNPPEPFLAGMMYGFLGQKDRAFYWLERGYEDRSPAYSSLNVDPCWDPLRDDPRFKDL
                     VRRVGLP"
BASE COUNT         1422 a         2151 c         1779 g         1515 t
ORIGIN      
        1 cgttattttt tcctgtatgt tgtaaccgga ggcttcggtt ttctggccag cgcttttctc
       61 gggcactact cgctgggcgc cagcggcgct ctcctgggcc tggtcggcgc aatgctcgcg
      121 gtaaccacca agcgtggcgg cgcgttcatg cgtgaccttc gcgcgcgttt gatcagttcc
      181 gtcgtcatcc tgttcatgct gggccttgcg ggaatgggat tggacaatac ggcgcatttt
      241 gcggggctgg tagccggctt tgccctcggg aaaatatatg ccgaccgcca gccgatgaac
      301 gtcagcgagc ttcgccgcgc ctacgccatg gggtggctcg ccggagtcgt cgtagtcgcg
      361 agttttgtgc taatgctctt gcattatcgc gatccgattt ccggttagaa gcagctctaa
      421 atcgtccgtt cactcttggt gcatcctgaa gcacgggcaa gcggccgatc cgcttgtttt
      481 aagttacgct gtaatcgacc attggaaccg cattccatcg tcgtcgtcag cctgcacact
      541 cccaaggaaa aattctgggg tgaattgctg gccatcagca ccgccggcat caccctgcgc
      601 ggcatcgatc tgaattcctt cgatcatttc gtcaaccaga tcaacggtgc cgaaggcgaa
      661 cgcactggcc tgcccacact cttttttcct atgacccgtg tcgagcgtat tgctctcgac
      721 gaaccttccg gctccatccc gtcactctct gaagtgttcg cgcgcaaagt cggccgcccg
      781 ctcgccgaat acctcgccca attcgcctag gacttcagtt ttaaacatag cgccggtccc
      841 ttcgcgcggc atcttccgtt tgccgcttat gcgacaacca tctccaactg cgttatcatt
      901 ttttcaagga aattcccgag ctaacaatga agggtcgctt ctggacggta cccaatcaaa
      961 tcacgctgct caggctcggc atcctgccgt tctttctcat ccttattgcc tatgagcatt
     1021 accgctacgc tctgctgatc ctggtcctgg ccggctgctc cgacggcatc gacggcctgc
     1081 tcgcccgcaa actcaaccaa aaatcggcca tcggcgcata tctcgatccc atagccgaca
     1141 aacttctact ttcgtcgtct tttatactgc tggcgatgga aaaacagctg gcctggtggc
     1201 tcaccatcat ggttctcagc cgcgacatca tgatgctcac tgtcgcagcc gtcataatcc
     1261 tgatttctgg ctatcgtccg tttccgccaa gcctgctcgg caaagccacc acgctgttcc
     1321 agattatttt ggtgttcgtc gtggtgctgc aggctggcta tcccgacctt ggcctgacca
     1381 acgtcgttcg cttcctgatg tacttcgtcg cagtgatcac tacggtgtcc ggcttccatt
     1441 attcctttgc catcgcccgc cgcctcagct cctgacagtt tcaaaaattc gtcattccga
     1501 gcgaagcgag aaatctctcg tcgattcctg ggagacctta tccagccatc gccgccggtc
     1561 cgccgggtgg caatattaac ttcggctccg tcagcgcctg cacctgctcc acggtcgtgt
     1621 ccggcgccag ttcccgcagc accaatcctt ccgccgtaac ttcaatcacg gccagttcgc
     1681 tgacgatgtg atccaccact tcgacaccag tcagcggcag cgagcatttc gtcaaaattt
     1741 tcggctttcc gtctttcgtc gcgtgttcca tcgccacgaa cacccgcttc gcacccgcca
     1801 ccagatccat cgcgccgccc atgcccttca gcatctttcc cggcacggcc cagttcgcca
     1861 gcgttccttt ttcgtctacc tgcagcgcgc ccaacaccgt cagatcaatg tggccaccgc
     1921 gaatcatcgc gaacgaatcc gccgacgaaa aataacagca gcctttcagt tcgctgatgg
     1981 tctctttccc ggcattgatc aaatcaggat caacttccga ttccagcgga tacgggccca
     2041 ctccaagcat tccgttttcg gactgcagaa tcacttccat ccccggcggc acatggttcg
     2101 ccaccagcgt cggtattcca atgccgagat tcacatagaa gccgtcacgc agctcccgcg
     2161 cgatccgctt cacaatccgt tctcgctttt gttcttcagt catcatccga gcgagtgcgc
     2221 tttccacatc agtttgagag ttggcatata aagagcaagc accaccaagc taggcagcac
     2281 aaccgccgcg aagaacagat aagcatgcat tgcccgcctc tgcgccctgt tgagcgggat
     2341 tatctgtctg acaaccgcaa gggcgatcgc cataaaggaa aaaaacggca gagcccacca
     2401 ataactcagg agaagaagcc tggtaggcca gaggatttca acctgcaagc cagcaaataa
     2461 attggagaaa acgggaacaa ccttgataaa ggcagtcccg acgaaaaaca aatacaaaca
     2521 aaacacaacg gcaagcggca aaggcagacg cgaattggat ttcgcatttg ccgcgaaagt
     2581 gtccgtttga ttggtcgcac cttttacgtg catacgaacc tcgcgactca accacgcagt
     2641 ctggtaagtc acacctttcg tgtagtccgc cgctcaattc gcttctcgta cttcggccct
     2701 tggaaaatcg cgtttacgta caaaccgggc gtgtgaacac tgtcggaatc gagcgagcct
     2761 aattccacca gctcttccac ttccgcaatc acgtaatccg ccgccgtcgc catcatcgga
     2821 ttaaagttcc gcgcggtctt gcggtagatc aaattccccc agcgatcgcc cttccacgct
     2881 ttgatgaaag cgaagtctcc gcgaattccg cgttccatca catactgccg cccctcaaac
     2941 tcgcgcgtct ctttcccctc agccaccagc gtgccgtgac ctgtcggcgt ataaaacgcc
     3001 ggaatccccg ctccgcccgc gcgaatccgc tccgcaaaag tcccctgcgg attcaactcc
     3061 aactccaatt ccccactggt cacgagctgt tcgaacaatt tgttctcgcc aacgtatgtc
     3121 gaaaccatct tcttcacttg ccgcttttcc agcaaaatcc caatcccaaa tccatccacg
     3181 cccgcattat tcgaaacaac agtgagattc ttcgttccct tgcgttgcac cgccgcaatc
     3241 agattctccg gaatcccgca cagcccaaac ccgcccatta gaatggtcat cccatcctgc
     3301 aggttggcta cggcttcatc cgccgaagag atgcatttgt ccatcagagt tcaaattccc
     3361 ttaagcagtt tatacagttc gttggcggcg tcgaagtcgc cttcgaggtc gcgagagccc
     3421 agccgttcac ggagttcgtc gatgcgtcgc gcaaagagat cgagcgaagc ggaaagaatt
     3481 tcgcggttgg tcaacacaat gtcgcgccac agcgtatacg aactcccggc taggcgcagc
     3541 gagtcgcgca agcccgggcc cgcaagggtg atgggtaggc cattttcatc aaggcggtca
     3601 tagagaaatg atgccagtgc aaccgccgcc agctgcggca gatgtgaagc gaggccgacc
     3661 gcgtagtcat gttgttgcgc gccgatccac atcgggcgag cgccgatttt ttccagaatt
     3721 ttaacaaacg cgccgacgcg aggatcggat tcagggctgt gccgctgtcg cgaaagaagc
     3781 ggctcttccg gaattccggg cggcatggca atggcagact gagccgaagc agcgtctgca
     3841 accgcggcgg cttctgcagc agtggcgatc aaagcggcat cggaagatgg ctcggtgatg
     3901 agcgcgtacg gatgatcgcg aaagagatcg ctgtcggctg cggcgattcc cgaaagttcc
     3961 ttgccggcca tgggatgtcc gccgaggaag agcgtgcccg cgccgccggg aaaaagcgcg
     4021 gctgcgtcct gcgtgatgcg cagcttggtg ctgcaggcat ccgtgatcag cgcattaggc
     4081 gatgcaaaac gtgcgatttc tggaagcaga tccagcgtcg cgccgatcgg gagagcaata
     4141 taaatcagat cggcgccgtc caatgcctcc ttgaccgaac cggaagagct ctggtcaata
     4201 atgcggcggc catgtgcctc attgacgaca gacgcgcgat cccagccacg aacgaaaata
     4261 tccgtcgtgt atttcttcag cgcgagcgcg aatgagcccc cgataaggcc ggtacccaga
     4321 accgtgacgc gttgaatgag atggggagac ataaaggaga gagtttacag cctgccgccc
     4381 aaaatcgaca aagcgccatc aacaaagatt catttctgct tccgctcgaa cactggctct
     4441 aaggcaatcc cacacgccga accagatcct taaaacgggg atcgtcgcgt aagggatccc
     4501 aacatggatc cacatttaat gaggaatacg cggggctacg gtcctcatag ccacgctcca
     4561 accagtaaaa cgcgcgatcc ttctgaccca ggaatccgta catcatcccg gcaagaaagg
     4621 gctcaggagg attgcctcgc gcatcggctg cttccattcc ggccactagc gcggtgatcg
     4681 cgccattgta gcctcccctc tccaggccac ggtgcatgct gtccgctaat tcggtgtagc
     4741 ccaacatacg aatagcctgt tcccactcgc gcgcggcgtc ttggcggcgc ccggcccctt
     4801 cgtagtcata tgccagattc cagtgcgacc aaaatccgaa ggcctgccgc ttcacttccc
     4861 cttcgtcctc ctcgatcgcg cggtcatact ctcgccgcct atagaacgga ttggggttga
     4921 agtcgtgccc cggatctaag ctctgagaca ggaggaattc cttcatgcct tcgtcaaacc
     4981 gcctcatgca atccaagtat tcggcatacc gcgcatgggc ttccgcggag ttgggattga
     5041 gttgtatcgc gcgtctgtat tcctgctctg cttcggacca gcgccagagc cgcaggacat
     5101 cgagcgtcgc cagcgcctcg tgcgcttcgg ccagctcggg gtccagcgcg atgcctttcc
     5161 gaagaagcgc ttccgctttc tccggaccgt cgtccgaaac cggttcatct tgccaggtct
     5221 gcgccaagcc gacatacgct tgcgcataag cgggatcttc ctgaatagcc ttttcgaaga
     5281 agccccgtgc gtccgccagt tcgcgttgtc tgaccgctct ttggccgttt cgataagtca
     5341 cggctgcagc gcgctcggaa tgatagcggc cctggagaaa cagttcgagc gcgacgacag
     5401 acaacggctt gctgcgcgcg aggcgcgctt tttcgcgagg cgtcaaggtc gcgcgaatct
     5461 cttccgcgac agcccttgca atgtcctgct ctagtgccag gacattctgc gagtcgcgct
     5521 cgtaacttct ggcccacaaa tgacgatcgt ctggcgcgta gatgagctgg gcggtaactc
     5581 gaatggaatc acccgatttc tgtactgtgc cttcaacgat gccgttcacg cccagctcac
     5641 gcgcgacctg cgacaacggc ttgttcgtgt tgcggtaccg ctccgccgac gtccgcgaga
     5701 tcactctaac cgaagaaatc tgggagagct ctgtcgtgag tgcgtcagtg aggccgtcgc
     5761 tgaaatactc ctggtttgga tccgcggaga gactggtgag cggtagtaca gcgagcgatg
     5821 aaattttagg ggtgccagag agtttggata agtgcccgcg gacgtttttc acatctacgg
     5881 caaggagcaa tgccgcaaac acgactgtag ccgttgccat cacgacagct ttgcgaatag
     5941 cgctcggcgc ttttgctgag tttacagcag agtagctccc ctccgaagga ccaacacgaa
     6001 ttgaggggcc gccggcagta acaggcgcga tcggcgcaat tagcctgtac ccgcgcttcg
     6061 ggatcgtctg gatgaaccgc gattgccggg ggtcgtcttc gaatacccgc cgcaattccg
     6121 acacgcaccg ggtcaaaaca tcatccgtga cgaatgtgcc gggccacacg acctccagca
     6181 gcttttcctt cgaaatggtc tcgcccgggc gaccggccag acacaccagc acctccatca
     6241 ccttcggctc aatacggacg ttcttaccgt tgtgtgacag cgtgttcagg cttggtactg
     6301 ctacccaggg gccgatctgg aaagcgtctg ccataagccc attgccggcg tgcttttggc
     6361 tggattatat acctgtttcc ttgttcgggg ccgtatccga ctgctcttcg ccctcgggaa
     6421 agcctctgct cttcctcacc tgctcctcag gactgtcgct catttatacg agaagttcag
     6481 tacgcgagtc ttcttttcgc tcgtggacga gcccattgta gaaccgtgaa agaagaggag
     6541 ttcgcctttt ggagagccca cccctcttta aggtgctgtt cctgatcgcg cgggcgctcg
     6601 cccgttccgg gacgcctccg tccaacgcgg cggattgtgc cactaccggt actggccccc
     6661 agcgaggatt cacattaacg ggagacataa tcttgcctcc tggtaccgct cccagtgccg
     6721 tcatcgagtc tgctgatggc aaggtcacct caactgaaga tcatcccgtg agcggtcaat
     6781 atgcggagat gtctggcacg atccgcgcca gtgcggactg cgcgacctcg gtgacgctca
     6841 acttttacca ggcgggccaa ctggtcc
//
LOCUS       DASTPM010000697         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_111460, whole genome shotgun sequence.
ACCESSION   DASTPM010000697 DASTPM010000000
VERSION     DASTPM010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111460"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFR42_12355"
     CDS             complement(<1..714)
                     /locus_tag="VFR42_12355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0370000.1"
                     /translation="MRYLALATDYDGTLATNGVVNHATVRSLEKLKASGRKLLMVTGR
                     HLPDLQRVLQKLELFDRVVAENGALLYSPASREEKVLAEAPKKEFLQALRAAGVIFDV
                     GHCIVASWTPSEVAILEVIKRLGLDYQVIFNKGAVMVLPAGINKATGLKAALEELQLS
                     PHNTVAIGDAENDHAFLSASECGVAVANALPALREKADVVTRGDYGAGVEELIDQLLK
                     DDLQAWDERLERSSIAIGTP"
     gene            886..1338
                     /locus_tag="VFR42_12360"
     CDS             886..1338
                     /locus_tag="VFR42_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370001.1"
                     /translation="MPEPSATPKEKRGRRVFTAGAVLLVILGLVHSLSLIKAPVPSND
                     AERQLLDLMSNYKFDLMGSQRTMDNMFRGFSISFMLAALVLGLLDLVLRRERTQLLKR
                     IALVNVLWLVAMTGVSLRYFFAAPTSFLVAALLVFLLAWLTLPSTAIN"
     gene            1391..1798
                     /locus_tag="VFR42_12365"
     CDS             1391..1798
                     /locus_tag="VFR42_12365"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEU0370002.1"
                     /translation="MADLTVLFDGSCTLCRASADRVRRFDSRGRIELLDLHDPSTTQR
                     FPQVNREEAMKWMQAVDPQGRISTGADAWARIGSLLPGWNLLAWILLLPGIRWLAARI
                     YAWIARNRYRWNRTICEDGSCSLHVKKGPSPRS"
     gene            1808..>2303
                     /locus_tag="VFR42_12370"
     CDS             1808..>2303
                     /locus_tag="VFR42_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0370003.1"
                     /translation="MRIAIVSDIHGNLTALEAVLADLRETSPDMVFHGGDLADGGARP
                     AEVVDRVHGLGWPGVIGNTDEMLARPETLADFAAQSPPTLQPLFAVIKENAAATREAL
                     GEDRVAWLGSLPRAQIQEQFALVHASPESCWRAPGPEATDAELESVYRPLGKTFAVYA
                     HIHRP"
BASE COUNT          418 a          774 c          601 g          510 t
ORIGIN      
        1 cggcgtcccg atggcaatcg aagagcgctc caaccgctcg tcccacgcct gcaaatcgtc
       61 cttcaacagt tgatcgatca actcctccac gcccgctccg tagtcgccgc gcgtcaccac
      121 gtccgccttt tcccgcagcg ccggcagcgc attcgccacc gccacgccgc actcgctcgc
      181 cgacaaaaac gcgtgatcgt tttcagcgtc gccgatggcc acggtgttgt gcggcgacaa
      241 ctgcaactct tccagcgccg ccttcaaccc tgtcgctttg ttgatccccg ccggcagcac
      301 catcactgcg cctttgttga aaatcacctg gtagtccagt cccagccgct tgatgacttc
      361 cagaatcgcg acctcgctcg gcgtccagct cgccacaatg caatgcccaa cgtcaaagat
      421 aactccggca gcgcgcagtg cctgcaaaaa ctctttcttc ggcgcctccg ccagcacttt
      481 ttcttcgcgg ctcgctgggc tatacaacag cgctccgttt tctgcgacta cgcggtcgaa
      541 caattccagc ttttgaagaa cgcgctgcaa gtccggcaaa tggcgccccg tcaccatcaa
      601 cagcttccgt cccgacgctt tcaacttctc cagcgaacgc accgtggcat ggttgaccac
      661 gccgttcgtt gccagcgtcc cgtcataatc cgtagctaac gccaaatacc gcatcgcgcc
      721 tctcgctctt tgaaactgca agaacttagc atgaccattc gcgatacttc tctgcagcaa
      781 tcgctgtatc tctatggcgc gacgacccag cttctccacg actttcactt ttggcccaga
      841 gctgctgtgt gttacaacct tcccgcactc caaaggaggc gtcccatgcc cgaaccttct
      901 gcaactccaa aggagaagcg tggtcgccgc gtgtttacgg ctggagccgt cctgctggtc
      961 atcctcggcc tggttcactc cctctcgctc atcaaagccc cagttccttc caatgatgcc
     1021 gagcgccagc ttctcgacct catgtctaat tacaaattcg atctcatggg gtcgcagcgc
     1081 acgatggaca atatgttccg cggattcagc atctccttca tgctcgcggc cctggtcctt
     1141 ggcctgcttg acctggttct ccgccgcgag cgcacgcaac tgttgaagcg catcgccctg
     1201 gtcaacgtcc tttggcttgt ggcgatgacc ggcgtatcgc tccgctattt cttcgccgcc
     1261 cccacatctt tcttggttgc ggccctcctg gtcttcttgc tcgcctggct gaccttgccc
     1321 tcgaccgcca taaattgatc tggcggctcg agttggtctt agaatcagag tgttaggacg
     1381 tttccgcttc atggctgacc ttacggtcct tttcgacggt tcctgcacgc tgtgccgcgc
     1441 cagcgccgat cgcgtgcgtc gttttgactc acgcggccgc atcgaattgc tcgacttgca
     1501 tgacccctcg acgacgcagc gctttccgca agtcaatcgc gaagaagcca tgaaatggat
     1561 gcaggcggtt gacccgcaag gccgcatctc caccggcgct gacgcctggg cgcgcattgg
     1621 atcgttgctc cccggctgga atctgctcgc atggattctg ctgctgccgg gaatccgttg
     1681 gcttgccgca agaatttatg cgtggatcgc gcgcaatcgt taccgttgga accgcacaat
     1741 ctgcgaggat ggctcctgca gcctccatgt caaaaaggga ccgtcgccgc gttcgtgacc
     1801 attcaacatg cgcattgcca tcgtctccga cattcacggc aacctcaccg cgcttgaggc
     1861 ggtcctcgct gacttgcggg aaacttcgcc ggacatggtt tttcacggcg gcgatttagc
     1921 ggatggtgga gcgcgtccgg cggaagtggt cgaccgcgtt cacggcctcg gctggccggg
     1981 cgtgatcggc aacaccgacg aaatgctggc acggccggaa actcttgcgg attttgcggc
     2041 gcaatctcca ccgacgctgc agccgctttt cgcggtgatt aaagagaatg cggccgcaac
     2101 tcgcgaagcg cttggagaag atagagttgc gtggctcggc agtctgcccc gggcacagat
     2161 tcaagagcaa ttcgcactgg tgcacgccag tccggaaagc tgctggcgcg cgcccggtcc
     2221 agaagccacg gatgccgaac tggagagtgt ttatagacca ctcggcaaaa cgttcgcggt
     2281 ctacgcgcac attcatcgcc cgt
//
LOCUS       DASTPM010000698         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_400076, whole genome shotgun sequence.
ACCESSION   DASTPM010000698 DASTPM010000000
VERSION     DASTPM010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400076"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFR42_12375"
     CDS             complement(<1..740)
                     /locus_tag="VFR42_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0370004.1"
                     /translation="MNILGLNAYHGNASAAVVCDGTLVAAVEEERFNRVKYAAGFPAA
                     AIQYCLKQAGLKLEEIDHVAVPRNPYARLGTKLFYALRMPSFARGRAKVLVKFSGVKE
                     ALAAAFDIDPGAIRARFHRVEHHQAHLASAFLVSPFEQAALLSADGLGDFASTMWGTG
                     RGGHMDIEDAIAFPHSLGLYYTAVTQYLGFLKFGDEYKVMGLAAYGQPERMEVFRDIV
                     RFDANANGNGFRLGLDYFVHHRNGPEMS"
     gene            complement(814..>2285)
                     /locus_tag="VFR42_12380"
     CDS             complement(814..>2285)
                     /locus_tag="VFR42_12380"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEU0370005.1"
                     /translation="GFAFASEVRALLAGGMAPKQISQDALTSYFLFGSVSEPVTMLDG
                     VFSLPPGHRMLVYVPERRRMPRARAWWDATRSAAAKDGRATGDFASAVKSLRPLLQDA
                     VRAHLIADVPVGLFLSSGLDSSVIAALAARERAGICSFTLSFPGTNYDEAPLARSVAK
                     HCGTQHREIPLEGDAIVSRLDEAVSAFDQPSMDGTNTYFVSWAAREAGLKVALSGLGG
                     DELFAGYATFADTPRMLRLIRAAWFVPAPVRKAFASLLGTIMGSKIGPDAVRKAKAAW
                     VAPDALPHAYFFTRALFPVGTELSRLIEPRFRPSSVAADGVTLEPTWVGWLERAADGA
                     RKLEGTASVSWMEMRSYMASTLLRDTDSVSMARSLEVRVPLLDTPLVEFVGALPDAAR
                     RRNGIAKALLIESVGDLLPQEILAQRKRTFTLPWEQWLRGPLRKKMEASFADIAPALA
                     AHVHQDGVREVWNTFLAGKTSWSRPWSIYAVNEWCRRNL"
BASE COUNT          492 a          787 c          571 g          435 t
ORIGIN      
        1 caactcattt ctggcccatt ccgatgatgc acaaaatagt ccaggcccaa ccggaacccg
       61 ttgccgttcg cattcgcgtc aaagcgcaca atgtcgcgaa atacttccat ccgctcgggc
      121 tggccataag ccgcaagccc catcactttg tattcgtcac caaatttcag gaatcccaga
      181 tactgggtca ccgcggtgta atacagtccc agcgaatgtg gaaacgcgat ggcgtcttcg
      241 atatccatgt ggccgccgcg tcctgtgccc cacatcgtgc tcgcgaaatc tccaagcccg
      301 tccgccgaaa gcagcgctgc ctgttcgaac ggcgaaacca aaaaagcact cgccagatgc
      361 gcctgatggt gctcgaccct gtgaaaccgc gcccgaatcg cccccgggtc gatgtcgaac
      421 gccgctgcta gcgcttcctt tactccgcta aatttcacca gcaccttcgc tcgaccgcga
      481 gcaaacgacg gcatgcgcag cgcataaaag agcttcgtgc ccagccgcgc gtaggggttt
      541 cgcggcaccg ccacatgatc aatttcctct agcttcagtc ctgcttgttt caggcaatac
      601 tggatcgcgg ccgctggaaa tccagccgcg tacttcacgc gattgaaccg ctcctcttcc
      661 accgccgcta ccagcgtgcc atcgcacacc accgccgccg aagcgttccc gtggtacgca
      721 ttgaggccca agatattcat gagggtaggt tagtttacag cagcagactc tttccaggag
      781 gttccgggcg cacaccacca ccgatggcga tttctacaga ttccgccggc accattcatt
      841 cacggcgtaa atcgaccacg gccgcgacca cgaggttttc cccgcgagaa aagtgttcca
      901 aacttcgcgc actccatcct ggtgcacatg cgccgctaag gccggcgcga tatccgcaaa
      961 gctcgcctcc atctttttac gcaacggtcc gcgcaaccat tgttcccagg gcaatgtaaa
     1021 tgtccgcttc ctttgcgcta agatctcctg gggcagtaag tcgccgactg attcgatcaa
     1081 caaagccttc gcaatcccat ttcgccggcg tgctgcatcc ggcagcgcac ccacaaattc
     1141 caccagcgga gtatccagca gcggcacacg cacctccagc gaacgtgcca tgctcaccga
     1201 atctgtatcc cgcaacagcg tgctggccat gtacgagcgc atctccatcc aggagacgct
     1261 ggccgtccct tccagctttc tcgctccatc cgccgcgcgc tccagccagc cgacccacgt
     1321 cggttccagc gttacaccat ccgccgccac gctgcttggc cggaatcgtg gctcgatcaa
     1381 tctcgacaat tccgttccca cgggaaacaa tgcgcgggta aagaaataag catgcggcag
     1441 agcgtcaggg gctacccacg ctgcttttgc ttttcgcacc gcatctggac cgatcttact
     1501 gcccatgatg gtgcccaaca gggatgcaaa tgccttccgc actggtgccg ggacaaacca
     1561 cgccgcccga atcagccgca acatgcgtgg tgtgtccgca aaagttgcat aacccgcaaa
     1621 aagttcatcg cctccgagac ccgacagcgc tactttcaat cccgcctcgc gcgccgccca
     1681 cgaaacgaaa tacgtgttgg ttccatccat gctcggttgg tcgaacgcgg aaaccgcctc
     1741 atccagcctc gagacgattg catcgccttc caacgggatc tcacgatgct gcgtcccgca
     1801 atgttttgcg acgctccgtg ccagcggcgc ttcatcgtag tttgtgccgg ggaagctgag
     1861 tgtaaagctg cagattccgg ctcgctcgcg cgcggcaagc gccgcgatca cactggaatc
     1921 cagtccactc gaaagaaaca agcccaccgg aacatccgcg atcaagtgcg cacgcaccgc
     1981 atcttgcagc aagggccgca aactcttcac tgcggaagca aaatcgcctg tcgctcgccc
     2041 gtccttggcc gcagcacttc gcgtggcgtc ccaccaggcc cgcgcgcgcg gcatgcgccg
     2101 ccgttccggc acatacacca gcatgcgatg tcccgggggc agggaaaata ctccgtccag
     2161 catcgtcacc ggctcggaaa cgcttccaaa aagaaaatag gaggtcagcg cgtcttgcga
     2221 aatctgtttc ggcgccattc ctccggccag caacgcacgg acttcagagg cgaacgcaaa
     2281 ccctt
//
LOCUS       DASTPM010000699         2729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_688561, whole genome shotgun sequence.
ACCESSION   DASTPM010000699 DASTPM010000000
VERSION     DASTPM010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688561"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFR42_12385"
     CDS             complement(<1..591)
                     /locus_tag="VFR42_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370006.1"
                     /translation="MDEADAAREDSSHIPRISFVSYLRTVNECVADEPAARHLSTAPT
                     LAAGEQVPPSEPPNSEFQQLGYQGFVIARSRRQVLDILREGNSCSAWYAGAEPNPSIK
                     FASLHYRVDGEGEDTAVGDQTSFGLYYREPYVARAQQSVGAGSIITLNAHGAFFAGRS
                     PAKVYWDGGPVIRQTNKFLHVAMYPGGSLNAQVTTLL"
     gene            complement(1101..1709)
                     /locus_tag="VFR42_12390"
     CDS             complement(1101..1709)
                     /locus_tag="VFR42_12390"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0370007.1"
                     /translation="MLQAFRWLAFSAVAASVFSVPVSSQEQLKLFVGYSYLRPSVTYE
                     QTSTCPLGCPAVPGSVTKHPNLNGYEFSATYKFFPFVGVTADFSGHYGTVTGSSSAHV
                     QTYLFGPELAFPAKVSPFVHALFGVAHQAVGSGTSSSNGVPVDVFSASDNAFATALGA
                     GIDLHIAPFLSFRAIQLDYLITRFHSSTQNQPRASTGLVLRF"
     gene            complement(1794..1925)
                     /locus_tag="VFR42_12395"
     CDS             complement(1794..1925)
                     /locus_tag="VFR42_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370008.1"
                     /translation="MIKKVAGGYKVLSERGKNLGGPYKTKKEAEKRLRQVEFFKHKK"
     gene            complement(2057..2506)
                     /locus_tag="VFR42_12400"
     CDS             complement(2057..2506)
                     /locus_tag="VFR42_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370009.1"
                     /translation="METKEHKTYAFLLSAAALDDFLAAWEAGTLPRSQWIHAAHVAVA
                     ACYAFDLTPADALFRTRRGIIHFNNCVGTPNTDTRGYHETLTRLWSGIIGDFVRRGNF
                     PSRFAAASAAVERYGEDRGRHRLHYSFDVVNSVDARRNWVPPDRWPL"
BASE COUNT          583 a          732 c          862 g          552 t
ORIGIN      
        1 cagcagtgtg gtgacttgag cgttcaagga gccgccggga tacatggcta cgtgcaaaaa
       61 cttgttggtc tgtcgaatca ccggaccgcc gtcccagtac acttttgccg gcgagcggcc
      121 agcaaaaaaa gcgccgtgcg cgttcagcgt aatgatcgat ccggcgccaa cactttgctg
      181 tgcccgagcg acgtacggct cgcggtagta cagaccgaag ctagtctggt cgccgactgc
      241 ggtgtcttcg ccttccccgt ccacccggta atgcagggaa gcgaacttga tcgagggatt
      301 gggttcggct ccggcgtacc aggcagagca agagtttcct tcacggagga tgtccagcac
      361 ctgccggcgg gaacgtgcaa tcacgaagcc ctggtagccc agctgctgaa attcgctatt
      421 gggcggttcg gatggcggaa cctgttcccc ggcggccaac gtcggtgcgg ttgataaatg
      481 ccgcgctgcg ggttcatccg caacgcattc attcaccgta cgcaagtacg aaacgaagct
      541 gatgcgcggg atgtgtgacg aatcctcccg ggctgcatcc gcttcatcca tacaaggtgc
      601 gcgctctgaa aggttcggaa ggcgtactgg cctgcggact tgtccgcttg cggcggcaga
      661 aaagaacaag gaaacgacgg cgcttatcca gatcgcgcgt gcgtgccggt ggaacatgaa
      721 ctcctctttc accgaccgat gcgtgcgccc attcgcttac ccatctaagg gcagcaagtg
      781 gcgaacgccg tacccaggta gcacgagaga cgaagccgcg acaggaaaag tttggttacg
      841 cgtaagtcaa accgtgatgc cagcgggtga tttagatgat ggattgatga gtcatcggga
      901 taacttgcgc agaatatttg taaaacccgt tcataagtgt gaggaaaagg aaaatcggtt
      961 cgtcattttg agattccgcc cctgcatctc gcacctgaca tagccgcctc agccccttgg
     1021 caccactgga tgctgcgaac cgcgacgttg ccgcgccggt atcgggcgag gagggcagtt
     1081 tggaggggtg atagattttt tcagaagcgc aggaccaagc cagtggaagc gcgcggctga
     1141 ttttgcgtac tggagtggaa acgggtgatc aggtagtcga gctggatggc gcgaaacgag
     1201 agaaaggggg cgatgtgcaa gtcgataccg gcgcccaaag cggtggcgaa ggcattgtcg
     1261 ctcgcggaga aaacgtctac gggaacgccg ttgctgctgg atgtgccgga gccgacggcc
     1321 tgatgcgcaa cgccgaacag ggcgtgaaca aacggcgaaa ccttcgcagg gaaagccagt
     1381 tccggcccga agagatatgt ctgcacgtgg gcggaactgg aaccagtgac cgttccgtag
     1441 tggccgctga agtccgcagt gactccgacg aacggaaaaa atttgtaggt ggccgagaat
     1501 tcataaccat tcaggtttgg atgtttggtc acggaacccg gtacggcagg gcatccaagc
     1561 gggcaggtgc tggtctgctc gtaagtaacc gaaggccgaa gataggagta accgacgaag
     1621 agtttgagct gttcctgcga gctgaccgga accgagaaga cagacgcggc aacggcgctg
     1681 aaagcgagcc agcggaatgc ttgcaacatt agcgcacctc gagtgtagat tcacacgggc
     1741 agggcgcgcg atctgcagta gggatgcgcg gcgggatccg ggcgttgctc ccgtcatttt
     1801 ttgtgtttga agaattccac ctgacgcagg cgcttttccg cttctttttt ggttttgtag
     1861 ggaccgccga ggtttttgcc tctttcggat agcactttgt atccgcccgc cactttcttg
     1921 atcatgagtc gccctcccgt tggtctgccg ggcccaggtc agcgtgcgtg cgttcgccgg
     1981 acgccggcca taccgtttct tgcgcaggtt gggcggaata aaactctagc ggaagtttcg
     2041 ctgtacgttt tgggatttag agcggccagc gatccggcgg aacccaattt cttcgcgcat
     2101 ccacgctgtt gaccacatcg aagctgtaat ggaggcgatg ccggccgcga tcctcgccgt
     2161 aacgctcgac ggcagcactc gccgcggcaa agcgcgaggg gaagttccct ctgcggacaa
     2221 agtccccgat gatacccgac cacagacgcg tgagcgtttc gtgatagccg cgagtgtccg
     2281 tgttcggtgt gccgacacag ttgttgaaat ggatgatgcc gcggcgcgtg cggaacagtg
     2341 cgtcagccgg ggttagatca aatgcgtagc aagcagccac ggccacgtgc gccgcatgga
     2401 tccactggct gcgcggaagc gtgccggcct cccaggcggc gaggaaatcg tccagcgcgg
     2461 ccgcggagag caggaacgca taggttttgt gttcttttgt ttccattgta tggcagcaat
     2521 tccagattgc tttcctaagc aatatagtgc attatgctgc gaatcatttg tccaagtcaa
     2581 ctcggagcaa agagtaccaa tggacgacct agatcgcaag acgctgcatt tcctcatgcg
     2641 caatggccgc ggcacttggg ccgaattggg tgagctgctg gggctttccg cgccggctgc
     2701 tgccgaacgt gctcgcaaac tggaagcgg
//
LOCUS       DASTPM010000700         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1409519, whole genome shotgun sequence.
ACCESSION   DASTPM010000700 DASTPM010000000
VERSION     DASTPM010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409519"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VFR42_12405"
     CDS             complement(<1..745)
                     /locus_tag="VFR42_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU0370010.1"
                     /translation="MAETKKNVGRVVQVIGPVLDIQFEEGHLPAIYNAVRVTSEGYNV
                     PEPLDVTAEVQQHLGEGRVRAVAMEPTEGMVRGMKAVDSGDSIKVPVGRQTLGRVMNV
                     LGKPVDQLGPINAETYFPIHRPAPKLEDQSTTLEMFETGIKVVDLMEPYVKGGKIGLF
                     GGAGVGKTVLILELIHNVAMKHGGLSVFAGVGERTREGNDLWLEMSEGGVITPGDTEK
                     SRCALVYGQMTEPPGSRLRVGLTGLTVAEY"
     gene            complement(755..1627)
                     /gene="atpG"
                     /locus_tag="VFR42_12410"
     CDS             complement(755..1627)
                     /gene="atpG"
                     /locus_tag="VFR42_12410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU0370011.1"
                     /translation="MPTLLDFRRRIRSVKNTQQITRAMKFVAAARLRRAQESALAARP
                     YATELARLLRSIMGRIDEPQHPLLAERPEERILAIVLTGERGLAGAFNTNILRKANEF
                     FREHKDKKLSVVPVGKKGRDTLKRAGFSFAAEYINVLAKVEFGTAREIAGLVSKLYKE
                     DQIDAVYLIFSEFKNVMTANLKVEKLLPVEPERSEGGDAKQPGSQVDYIYEQAPEKLL
                     GALLPKYIEAQILRAMLESSAAEYAARMAAMESATRNAGEVIQALTLHMNKVRQAAIT
                     KEIIEIVSGASHGA"
     gene            complement(1631..>2908)
                     /gene="atpA"
                     /locus_tag="VFR42_12415"
     CDS             complement(1631..>2908)
                     /gene="atpA"
                     /locus_tag="VFR42_12415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU0370012.1"
                     /translation="DTVKRTNTIMSVPAGEALIGRVVNPLGEPIDDKGPIAATQRNPL
                     ERIAPGVLDRQPVREPLQTGIKAIDAMIPIGRGQRELIIGDRQTGKTAVILVTIINQK
                     GQGMICIYCAIGQKRSTIAQVVKALTDAGAMEYTIVVASSAAEPASLQYIAPYAACAM
                     GEYFRDTKRHAVTFYDDLSKHAQAYREISLLLRRPPGREAFPGDVFYLHSRLLERAAK
                     LNDKLGAGSLTSLPVIETQAGDVSAYIPTNVISITDGQIYLEADLFNSGIRPAINVGI
                     SVSRVGGNAQIKAMRQVAGTLRLDMAQFRELAAFAQFGADQLDKVTQAQLGRGQRLTE
                     ILKQDQYVPLPVEKQVLSLFIATSGYLDNIPVPDVRRFEKEFLQFAETSHPGILQSIA
                     EKKALDDGSKGEIKKAADSFKERFTTSQAAAAK"
BASE COUNT          515 a          860 c          872 g          661 t
ORIGIN      
        1 aatattccgc gacggtcaga ccggtaaggc cgacgcgcag acgcgagccg ggcggttcgg
       61 tcatctggcc atagaccaga gcgcagcgcg acttttccgt gtcgcccggc gtgatgacgc
      121 cgccttcgga catttccagc cacaagtcgt tgccttcgcg ggtgcgttcg ccgacgccgg
      181 cgaacaccga aaggccaccg tgtttcatgg cgacgttgtg aatcaattcc agaatcaaaa
      241 ccgttttgcc gacgcccgcg ccgccgaaca atccgatctt gccgcccttt acgtacggct
      301 ccatcaggtc aacgaccttg atacccgttt caaacatttc gagcgtggtg gactgatctt
      361 ccagtttcgg cgcgggacga tgaatcggaa aataggtttc cgcgttgatc ggaccaagct
      421 ggtccacggg cttgccgagt acgttcatca cgcgaccgag cgtctggcgg cccaccggaa
      481 ctttgatcga gtcgccggag tcgacggcct tcataccgcg aaccatgcct tcggtcggtt
      541 ccatggcgac ggcgcgcacg cggccttcgc ccaggtgctg ctgcacttcc gccgtcacgt
      601 ccagcggctc gggaacgttg tagccttcgc tggtcacgcg caccgcgttg taaatcgcgg
      661 gcagatgccc ttcttcaaat tgaatgtcga gcaccggtcc aatgacctgc accactcggc
      721 ccacgttctt tttcgtttct gccattctgt ctccctacgc gccgtgagaa gcgccgctga
      781 caatttcgat gatctctttc gtaatcgctg cctggcgaac tttgttcatg tgcagcgtca
      841 acgcctggat gacttcgcct gcgttgcgcg tggccgattc catcgccgcc atgcgcgcgg
      901 cgtattccgc tgctgagctc tcaagcattg cgcgaaggat ttgcgcttcg atgtacttcg
      961 gcagtaacgc gcccaacagc ttttccggcg cctgctcgta gatgtaatcc acctgcgatc
     1021 caggttgctt ggcgtcgcca ccttccgacc tttccggctc gactggcagt agtttttcca
     1081 ctttcaggtt cgcggtcatt acgtttttga attcgctgaa gatcaggtat accgcgtcaa
     1141 tctggtcttc tttgtaaagc ttgctcacca agccggcaat ttcgcgagcc gtgccgaatt
     1201 cgactttggc aaggacgttg atgtattccg cggcaaagga aaacccggcg cgcttcaaag
     1261 tgtcgcggcc ttttttgccg acgggaacca cggaaagttt cttatcttta tgctcgcgga
     1321 agaactcgtt ggctttgcgc aagatgttgg tgttgaacgc gccggccaag ccgcgttcgc
     1381 cggtcagtac gatcgcaagg atacgctctt cgggccgttc ggccagcaga gggtgctgcg
     1441 gctcgtcaat tcttcccatg atcgagcgca gcaggcgcgc cagttcggtc gcgtatggcc
     1501 gcgccgccag cgccgattcc tgcgcgcggc gcagccgggc cgcagcgaca aacttcatcg
     1561 cgcgggtaat ctgctgggtg tttttcaccg agcggatgcg gcgtcgaaaa tcgagaagtg
     1621 tgggcatgga ttacttggcg gctgctgcct gcgatgttgt gaaccgttcc ttaaacgaat
     1681 cggccgcctt cttgatttcc cccttgctgc cgtcgtcgag cgctttcttt tccgcgatgc
     1741 tctgcaaaat tcccggatgg ctggtttccg cgaactgcag aaattctttt tcaaagcggc
     1801 gcacatccgg caccggaata ttgtcaaggt agccgctggt ggcgatgaac aggctgagca
     1861 cttgcttttc gacaggaaga ggaacgtatt gatcctgttt gagaatttca gtgaggcgct
     1921 gtccgcggcc aagctgtgcc tgcgtgactt tgtccaactg gtcggcgccg aactgcgcga
     1981 aggcagccaa ttccctgaac tgcgccatgt ccaaccgcaa cgtgccggcg acctgtcgca
     2041 tggccttgat ctgcgcgttg ccgccgacgc ggctgaccga aatgcccacg ttgatggcgg
     2101 ggcgaatgcc gctgttgaat aagtccgctt ccagataaat ctggccgtcg gtaatggaga
     2161 ttacgttggt cggaatgtat gcggaaacgt cgccggcctg cgtttcgatg acgggcaggg
     2221 aagtgagcga gcctgcaccg agcttgtcgt tcagttttgc ggcgcgttcc agcaaacgcg
     2281 agtggaggta aaacacgtcg cccgggaacg cctcgcgtcc cggcggccgg cggagcagca
     2341 aggaaatttc gcgataggcc tgcgcgtgtt tggaaaggtc gtcgtaaaaa gtgaccgcgt
     2401 gccttttggt gtcgcggaaa tattcgccca tcgcgcatgc ggcgtaagga gcaatgtact
     2461 gcagcgaagc cggttcggcg gcggacgatg cgaccacgat ggtgtattcc atcgcgccgg
     2521 catcggtaag cgcctttacc acctgtgcaa tcgtcgagcg cttttgcccg attgcgcagt
     2581 agatgcaaat catcccctgg cccttctggt tgatgatggt gaccaggatg acggcggttt
     2641 ttcctgtctg gcggtcgccg atgatcagct cgcgctggcc gcggccgata ggaatcatgg
     2701 cgtcgatggc tttgatgccg gtctgcagcg gctcgcgtac cggctggcga tcgagcacgc
     2761 cgggcgcgat gcgctccaac ggattgcgct gcgtggcggc gatggggcct ttgtcgtcaa
     2821 tcggttcgcc gagcggattg accacgcggc cgatcagcgc ttcgcccgcc ggcaccgaca
     2881 taatcgtgtt agtgcgcttg acagtgtc
//
LOCUS       DASTPM010000701         5974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_717540, whole genome shotgun sequence.
ACCESSION   DASTPM010000701 DASTPM010000000
VERSION     DASTPM010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717540"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VFR42_12420"
     CDS             <1..759
                     /locus_tag="VFR42_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0370013.1"
                     /translation="ERIGYMSQNFSLYPDLTVTENLTFYGRIYSLSAQRLKNRMQEVI
                     ALNGLGPYLNRLAAQLSGGWKQRLALGCAMLHEPKLLFLDEPTAGIDPVARRQLWDLL
                     FELSGHGITFFVTTHYMDEAERCSHVAYIYYGKLIADGTPNSLRELPDVQPPGTIRVE
                     ITTPEVTRALAHARQIKGVHSATIFGQSIHALIDDHLDLDALREQLSRNGATVGEIRP
                     LAPSLEDVFVELTYKNQAHGNNPEGSGATEAKHA"
     gene            752..1873
                     /locus_tag="VFR42_12425"
     CDS             752..1873
                     /locus_tag="VFR42_12425"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0370014.1"
                     /translation="MRDIFRGFGAVFYKETLHVRRDFGTLFFSLIIPLLQMLLLGYGI
                     DTNIRQINTVVFNADGRRESRELLDRLKNSDTFHVVRYVTSDDDMNNMIIGGKARVGI
                     KIPVDYSDKLLHQMSAQVLVLIDGSDASVAGQAINVSTAIGLDESLRRVLLNNASFAV
                     DMRPKLLFNPDSRSPNFFLPGLTAILLLNVTTFLTAFSIVREKERGTLEQLFVTPVKP
                     MGLLLGKLLPYLGIGFFELLLILTFMRFAFHVPIHGSVFLLAFLSLPYIFVSLSLGIL
                     ISSKANTQSEAMQLAFLTILPSIFFSGYIFPRETMPKVFYAISYLVPASYFINITRGV
                     ILRGAGIRHLWLDGLALFAIGAFLLIIAARRFHNKVIMA"
     gene            1989..2627
                     /locus_tag="VFR42_12430"
     CDS             1989..2627
                     /locus_tag="VFR42_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370015.1"
                     /translation="MNCPICEKRKAERYCPAKGEKICAVCCGTQREVTISCPPDCAYL
                     IAAHRYEDEHKRVLPEDTPLLDERIPEEILHTHQQLMAALAFQTAKFASNQPSTADPD
                     VLASLAALAGTYKTLQSGLLYEKVPEIPVQRDLYLALSQFLNEIKQKAAESGNSAALK
                     DGAIFHVIIFLYRMGLLRSNGRPRSRRFIEFLRGQFPQAEELKREEPRIIVP"
     gene            complement(2669..3697)
                     /locus_tag="VFR42_12435"
     CDS             complement(2669..3697)
                     /locus_tag="VFR42_12435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU0370016.1"
                     /translation="MTDAAEGAPAPLVGIARLASISPMAPTRRKSEERPEYFFLPVKS
                     LLNRCESNRVPFDWTINPYRGCEFACKYCYARYTHEFMEMDGTAFEKKIYVKKDAAVL
                     LARDIATKYKFAAGQNGAPEHICLGTATDPYQPAEREYGVTRACLEELARHEGLSVSI
                     TTKSNLITRDIDVLRQIAGRSRLWVDMTVTTLRPRLARLLEPRAPRPDLRLAAIQKLR
                     EAGIQVGAMASPLLPGITDREGELEAVAAAAKDVGAQWFVSGVLFLMPSSAKQFLPFL
                     REKFPRLVRQYQDWYVRNAYAPEPYRQKIAERVGRIKNHLGLTARPWQEMQQPVGSRA
                     QLSLSLAG"
     gene            complement(4061..4717)
                     /locus_tag="VFR42_12440"
     CDS             complement(4061..4717)
                     /locus_tag="VFR42_12440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0370017.1"
                     /translation="MTPEQILNRIGEIGIVPVVRAGSSQLALDAVEAIEAGGIPIVEI
                     TMTVPNAISTIRQVAARCGDRVLVGAGTVCNAHQARECLDAGAQFLVSPGLSVPVLEV
                     GRTLGVLAIPGALTPTEVMAAADAGARAIKIFPCGSVGGAKYLKALRGPFPDLHFIPT
                     GGVNPANAAEFFAAGAFAVGMGSDLVDAAALREGRKHEVIQSCREASGAVQVAKRKPR
                     "
     gene            complement(4758..5852)
                     /locus_tag="VFR42_12445"
     CDS             complement(4758..5852)
                     /locus_tag="VFR42_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU0370018.1"
                     /translation="MRILAKEGCRWDLVSLGEVMLRLDPGDLRVNTTRQFRVWEGGGE
                     YNVARGLRRTFGMKTAVVTALADNSVGRLIEDLMLQGGVHLDHVKWVKYDGVGRSVRN
                     GLNFTERGFGVRAAAGCSDRGHTAVSQLKPGEVAWEQIFSREGARWFHTGGIFCALSE
                     TTPLVAREAMEAAKKAGTGISYDLNYRPSLWQSIGGKAKAQEVNRSLASFVDVLIGNE
                     EDFSVALGFAVPGVDENLSKLDTAGFRTMMDAVRGKFPNVSLIAVTLRIAHTASRNDW
                     GAIACDAEGKFFEVATRRDLEIFDRVGGGDSFASGLLYGLLTGQDVPWSLECGAAHGA
                     LAMTTPGDTSMATFQDVAHLMKGQSARIAR"
BASE COUNT         1185 a         1923 c         1609 g         1257 t
ORIGIN      
        1 gagcgcatcg gctacatgtc gcaaaatttc agcctttacc ccgatctgac tgtcacagaa
       61 aatctcacgt tttacggacg catctacagt ctctctgctc agcggctgaa aaatcgcatg
      121 caggaagtca tcgcattgaa cggtctcggc ccttatttga atcgtctcgc cgcacagctt
      181 tccggcgggt ggaaacagcg gttagctctc ggctgcgcca tgctgcacga accaaagctg
      241 ctttttctcg acgaaccgac ggcgggcatc gatccagtcg cgcgccgcca attgtgggac
      301 ctgcttttcg aactttcggg ccacggaatc acgttctttg tgactacgca ctatatggac
      361 gaagccgaac gctgcagcca cgtcgcctac atttattatg gcaagctcat cgcggacggc
      421 acgccaaatt ccttgcggga gctgcccgac gtgcaaccgc cgggcacgat tcgcgtcgaa
      481 atcaccactc ccgaagtcac gcgcgccctc gcgcatgccc ggcagatcaa aggagtgcac
      541 agcgccacga tttttggcca gtccattcac gccctgatcg acgaccatct cgatctcgat
      601 gcactgcgcg agcaactttc cagaaacggt gcgaccgtcg gcgaaattcg cccgctcgcc
      661 cccagcctgg aagatgtttt cgtggaattg acctacaaga accaggccca cggcaataat
      721 cctgagggtt ccggcgcgac ggaggccaaa catgcgtgat atttttcgtg gattcggcgc
      781 cgtcttttac aaggaaacac tccacgttcg ccgcgatttc ggcacgcttt tcttctcgct
      841 gattattccg ctgctgcaga tgctcttgct cggctacggc attgatacca acatccgcca
      901 gatcaacacc gtcgttttca atgcggacgg ccgccgcgaa agccgcgaac tcctcgatcg
      961 cctgaaaaac tccgacacct ttcatgtcgt gcgttatgtc accagcgacg acgacatgaa
     1021 caatatgatc atcggcggca aagctcgcgt cggaatcaaa atcccggtcg actattccga
     1081 caagctgttg caccagatga gcgcgcaggt gctggtgttg attgacggtt ccgacgcttc
     1141 ggtggccggc caagccatca acgtttccac tgcgatcggt ctcgatgaat ctctccgccg
     1201 cgttctgctg aacaacgctt cgttcgccgt ggatatgcgg cccaagctgc tcttcaatcc
     1261 cgattcgcgc tcgcccaatt ttttccttcc cggcctcaca gcgattctgc tgctcaacgt
     1321 caccacgttt ctgaccgcgt tctccatcgt tcgcgaaaaa gagcgcggca cgctcgaaca
     1381 actctttgtc acaccggtaa aaccgatggg gctcttactc ggcaagctgc ttccgtacct
     1441 cggcatcggc ttttttgaac tgctgctgat cctcacgttc atgcgcttcg cgttccacgt
     1501 ccccattcac ggcagtgttt ttctgcttgc gttcctgtcg ttgccctata tttttgtttc
     1561 gctttcgctg ggcatactga tttcgagcaa agccaacact caatccgaag ccatgcagct
     1621 ggccttcctc accatcctgc ccagcatttt tttctctggt tatatcttcc cgcgcgaaac
     1681 catgcccaag gttttctacg cgatcagcta cctcgttccc gccagctact ttatcaacat
     1741 cactcgcgga gtgattctcc gcggcgcggg catccgccat ctatggctcg acggcctcgc
     1801 tctttttgct atcggcgcgt ttcttttgat catcgccgca cgccgctttc acaacaaagt
     1861 catcatggcc tgaacgtgta ttcgccccgg gactgagccg cagcaagttt tgtctttctg
     1921 gcaagcccgt cgtatcctgc cgcattgcgg ttaccatctg tcgtttcggc gaagtccagg
     1981 ggaatggttt gaactgtccg atctgcgaga aacgcaaggc tgagcggtat tgtcccgcca
     2041 aaggcgaaaa aatctgtgcc gtctgctgcg gcacccagcg cgaggtcacc atcagctgtc
     2101 cgcccgactg cgcctacctg atcgccgcgc accgctatga agacgaacat aagcgtgttc
     2161 ttcccgagga cacgccgctc ctcgacgagc gcattccaga ggaaatcctt cacacgcatc
     2221 agcagttgat ggctgcactg gcttttcaaa ccgccaaatt tgccagcaat caaccatcga
     2281 cggcggatcc cgacgttttg gcctcgctgg cggctctggc aggaacctac aagacgctgc
     2341 aaagcgggtt gctctacgag aaagtcccgg aaattccggt gcagcgcgat ctctacctcg
     2401 ccctgtccca atttttgaat gagatcaagc agaaggccgc cgaaagcggc aactccgcgg
     2461 cgctaaagga tggcgccatt tttcacgtga ttatctttct ttaccggatg ggtctgctgc
     2521 gttccaacgg gcgtccgcgc tcgcgccgct tcatcgaatt cctgcgtggc caatttccgc
     2581 aagctgaaga actaaaacgc gaagaaccgc gcatcatcgt tccctagctc gattccctcg
     2641 ctcgctttgt tcggctcgga agctgcgtct agcctgccag cgacaacgaa agctgcgccc
     2701 gcgatcccac tggctgctgc atctcctgcc acggcctcgc cgtcagcccc aggtggtttt
     2761 tgatccgccc cactcgctcc gcaattttct gccggtaggg ctccggcgca tacgcgttcc
     2821 gcacatacca gtcttggtac tgccgcacca gtcgtggaaa cttttcccgc aagaacggca
     2881 gaaactgctt cgccgacgac ggcatcagga acagcacacc cgaaacgaac cactgcgcac
     2941 cgacgtcctt cgccgcggca gccacagcct ccagttcacc ttcgcggtcg gtaattcccg
     3001 gcaaaagcgg cgaagccatc gcgccaacct gaattcccgc ctcgcgcaat ttttggattg
     3061 ccgccagccg caaatccggc cgcggtgcac gcggctccag caatcgcgcc agcctgggcc
     3121 gcagtgtcgt caccgtcatg tccacccaca gccgcgaacg ccccgcaatc tgccgcagca
     3181 cgtcgatatc tcgcgtgatt aggttcgact tcgtggtaat ggaaacgctt agtccttcat
     3241 gtcgcgccag ctcttccagg caagcccgcg tcacgccgta ctcgcgctca gccggctggt
     3301 acggatcggt agccgtaccc aggcagatgt gctcgggcgc tccgttttgc ccggctgcaa
     3361 atttgtattt ggtcgcgata tcccgcgcca gcagcaccgc cgcgtccttt ttgacgtaaa
     3421 tctttttctc aaacgcagtc ccatccattt ccataaactc gtgcgtatag cgcgcgtaac
     3481 agtatttgca ggcaaactcg cagccccggt agggattgat cgtccagtca aacggcacgc
     3541 ggttcgattc gcagcggttc aaaagcgact tcaccggcaa aaagaaatac tccggccgtt
     3601 cctcagactt ccgccgcgtc ggcgccatcg gtgaaattga agcgaggcgg gcgatgccga
     3661 ccagcggcgc cggagcgcct tctgctgcat cggtcaaagc aacagcagcc ttctggaaat
     3721 gcggcgtatc gcgaccagtt tcgtcgccac ccaccggaac gggcgggggg caatccgcac
     3781 catctttcgt ctccagagcg ctgctgcccg ggcccgagag aggggcaagc tcctcggccc
     3841 gttggttcgg gctacgttca gggctaacag gaaagagtaa agctgaggat ggtcgagcgc
     3901 gccggcgggc cgcaaagctc gtcggtgtag gggagggaaa ggaacgaaac actggcgaca
     3961 tagcccacct cgaaccaggc gagtatttcg ccttttgttc gctgtgtcaa gaactatttt
     4021 cgcctggtgt tcgcctgtgg ctttatacga gaactagctg ctagcgtggc tttctctttg
     4081 ccacctgcac tgctccgctc gcctctcggc aggattgaat cacttcgtgc ttgcgcccct
     4141 cgcgcagcgc ggcggcgtcg accaggtcgc tgcccatccc aaccgcgaag gctccggctg
     4201 caaagaactc ggcggcattg gcggggttca ccccgccggt gggaatgaag tgaagatcgg
     4261 gaaacggccc gcggagagcc ttgaggtatt tggcgccacc cacgctgccg cacgggaata
     4321 tcttgatggc ccgggctccc gcgtcggcgg cagccatcac ttcggtggga gtcagagcgc
     4381 ccggaatggc gagcactccc aaggtgcgtc ccacttccag cacgggcacg gagagcccgg
     4441 gactcacaag gaattgcgca ccggcatcaa ggcactcacg cgcctgatgc gcgttgcaca
     4501 cggttcctgc acccacgagg acgcgatcgc cacagcgcgc cgccacctgc cgaatggtgg
     4561 aaatggcgtt cggcaccgtc atggtgattt cgacgattgg gattccgcca gcttcgatgg
     4621 cttccaccgc atccaacgcg agctgcgaac tgccggcgcg cactaccgga actatgccga
     4681 tttcgccgat gcgattcagg atttgttcag gtgtcatgag ttaatggaat ctccaaccat
     4741 tcggcgtaaa caatcaacta ccgggcaatc cgcgcggatt gccccttcat caggtgtgca
     4801 acgtcctgga aggtcgccat ggatgtgtcg cccggcgtgg tcatggccag cgcgccatgt
     4861 gcggcgccgc attcgagcga ccatgggaca tcctgaccgg tgagaagtcc atacaataaa
     4921 cccgacgcga aggaatcgcc gccgcccacg cggtcgaaga tttccaggtc ccttctggtg
     4981 gctacttcga agaattttcc ttcggcatcg caggcgatgg cgccccaatc attgcggctc
     5041 gctgtgtgcg cgatgcgcag cgtgacggca atcagcgaaa catttgggaa tttgccgcga
     5101 accgcatcca tcatcgtgcg gaagccggcg gtgtccagtt tgctcaggtt ctcatccacg
     5161 ccgggaaccg caaaccccaa agcgaccgag aaatcttctt cgttgccgat gagcacgtcg
     5221 acgaacgagg ccagcgaccg gttcacttcc tgggctttcg cttttccgcc gatggattgc
     5281 cagagagatg gacggtagtt caggtcgtac gagataccgg taccggcttt tttggcggct
     5341 tccatggctt cacgcgccac caggggcgta gtctcggaaa gtgcgcaaaa aattccgccg
     5401 gtgtgaaacc agcgggcgcc ttcacgactg aaaatctgct cccaagcgac ctcgccaggt
     5461 ttgagttgcg aaaccgcggt gtggccgcgg tcggagcagc cggcggccgc gcggactcca
     5521 aatccgcgtt ccgtgaagtt caagccattg cgcacgctgc ggccgacgcc gtcgtatttc
     5581 acccacttca catgatcgag atgcacgcca ccttgcagca tcaggtcttc gatcaaccgg
     5641 cccacggaat tgtcggcaag cgcagtcacg acggcagtct tcatgccgaa agtccggcgc
     5701 agcccgcgcg ccacgttgta ttcgccgcca ccctcccaaa cacggaattg ccgcgtggtg
     5761 ttgacgcgca gatcgccggg atcgaggcga agcatcactt cgccgaggct gaccagatcc
     5821 cagcggcacc cttctttggc gagaatcctc acgcaggttc cctttgtttc gacaacggtc
     5881 ttcgtttcaa agcggctcgt tccgacaacg caggctccgc tagaccgcca gccggcgaag
     5941 ccgaaaggtt agcgcgaggg tccacgaaaa gcca
//
LOCUS       DASTPM010000702         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_544596, whole genome shotgun sequence.
ACCESSION   DASTPM010000702 DASTPM010000000
VERSION     DASTPM010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544596"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFR42_12450"
     CDS             complement(<1..748)
                     /locus_tag="VFR42_12450"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU0370019.1"
                     /translation="MDKFLITGGKPLHGTVAISGAKNSALPAMAAGLLTADSLLLHNI
                     PMVRDIITMGKLLAYMGATVATSEFPAANYTIASPIINEAVAPYELVKTMRASILTLG
                     PALARTGVARVSLPGGCAIGARPVDLHLAALEKMGAEIHTAHGYIEARAPRSGRLKGA
                     RIAFNKITVTGTENILMAATLADGETILENAAREPEVTDLVHLLRKMGAHIEGVATST
                     LTIRGVRSLRGAEHSVIPDRIEAGTFLVAGA"
     gene            complement(863..1783)
                     /gene="murQ"
                     /locus_tag="VFR42_12455"
     CDS             complement(863..1783)
                     /gene="murQ"
                     /locus_tag="VFR42_12455"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEU0370020.1"
                     /translation="MPTHSGKRPPEDRRRTEHRNPASRNLDRMSAAQIVHLMNREDRK
                     VAIAVNRELPSIARAADAIVHALQQGGRLFYVGAGSSGRMAVLDAAEIAPTFGTPPTL
                     VQALIAGGHKAVTSAVEGAEDAVRNGERDLRARKFTSKDIIVGIAASGTTPYVLGALT
                     YARKRTATIIAITSNRRMPITRLADIVIAPEVGPEVVTGSTRLKAGTSQKLVLNMLST
                     ATMVRLGHVYENLMIDAALTNKKLEDRALRILAEASGKSVSSGRRALRQARHDIRVAL
                     VILKRAVTAAEARKLLANAGGNLRQALRET"
     gene            1994..>3168
                     /locus_tag="VFR42_12460"
     CDS             1994..>3168
                     /locus_tag="VFR42_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU0370021.1"
                     /translation="MLKKLFEERFGVPAERVQPLQGELGASGRKIFRLCAGKTRAIGI
                     LYGVREENLAFLAFSRHFRRHGLPVPEIYGEDLERGAYLEEDLGDTTLFEFLSKHRDG
                     ENVTPEAIEAYRKVVEALPRFQVEAGRDLDYSVCYPCASFDRQSISWDINYFKYYFLR
                     LAGIPFNEQALENDFWRLTEFLLSANCDYFLYRDFQSRNVMLPEGQPYFLDYQGGRKG
                     ALQYDIASLLYDAKADLPPDVRQQLLDHYLDALQEHVQISREEFLHHYYGYVYVRIMQ
                     AMGAYGFRGFYERKAHFLQSVPYALKNVRWLLHNVTLAVPLPTLMDTFRNMLASEKLN
                     SLASEADDLVVRIFSFSFHKGMPKDESGNGGGFIFDGRSLPNPGREERFKQLTGKDGP
                     "
BASE COUNT          626 a          746 c         1109 g          687 t
ORIGIN      
        1 tggccccggc gacgaggaac gtaccggctt cgatgcggtc gggaatgaca gagtgttctg
       61 cgccgcggag agacctgacg ccgcggatgg tgagagtgga tgtggcaacg ccttcgatgt
      121 gcgcgcccat cttgcggagc aagtggacga ggtcggtgac ttccggttcg cgggctgcat
      181 tttcaagaat ggtttcaccg tcggcgagcg tcgcggccat cagaatattt tcggttccgg
      241 tgacggtgat tttgttgaag gcaatgcgcg cgcctttcaa gcggccggag cgtggggcgc
      301 gggcttcgat atagccgtga gcggtgtgga tttctgcacc cattttttcg agggcggcga
      361 ggtgaagatc gacggggcga gcgccgatgg cgcagccgcc gggaagcgag acgcgggcca
      421 cgccagtgcg agcaagcgcg gggccgagcg tgaggatgga tgcccgcatg gtcttgacga
      481 gttcgtacgg cgcgacggct tcgttgatta tgggcgaggc gatggtgtag ttggcggcgg
      541 ggaattcgga agtagcgacg gtggcgccca tgtaggcgag gagtttgccc atggtaatga
      601 tgtcacgcac catggggatg ttgtggagga gcaaagaatc ggcggtgagg aggccggcgg
      661 ccatggcagg gagtgcggag ttttttgcgc cgctgatagc tacggtgccg tggaggggtt
      721 tgccgccagt gatgaggaat ttatccattc ggttgttgca ccgtctccct gcgcagcaaa
      781 tcgtgaacct gatgatcaca tctaaaaaag agccgccctg aaggatggca actgcaatgg
      841 ccaaacgacg gccactactc gactacgttt cgcggagcgc ttggcgaaga ttgccgccgg
      901 cgtttgcgag tagtttgcga gcttcggcgg ctgtaactgc gcgcttgaga atgaccaatg
      961 ccacgcggat atcgtgtctg gcctgacgca gagcgcgtct gccgctggac acactctttc
     1021 cgctggcttc agccagaatg cgcagagcgc ggtcttctag cttcttgttg gtcagcgcgg
     1081 catcaatcat taagttttca tagacatgac cgaggcgaac catcgttgca gtggagagca
     1141 tattcaggac caatttttgc gaggtgccgg ctttgaggcg cgttgagcca gtgacgactt
     1201 cggggccgac ttcgggagcg atgacgatgt cggcgaggcg agtgatgggc atgcgccgat
     1261 tggaagtgat ggcaatgatg gtggcggtgc gcttgcgagc gtaggtaagc gcgccaagaa
     1321 cgtagggcgt agtgccgctg gcggcgatgc cgacgatgat gtcttttgag gtgaatttgc
     1381 gagcgcgcaa gtcgcgttcg ccgttgcgga cggcgtcttc ggcaccttct accgcgctgg
     1441 tgacggcttt gtggccaccc gcgatgagcg cctgaacgag cgtgggggga gtgccgaacg
     1501 ttggcgcgat ttccgcggca tcgaggacag ccatgcgacc gctggagccg gcaccaacgt
     1561 agaaaaggcg accaccctgt tgcaaagcgt gaacgatggc gtcggcggcc ctggcgatgg
     1621 aaggaagttc acgatttacg gcaatggcga ccttgcggtc ttcgcggttc atgaggtgga
     1681 cgatctgcgc cgcggacatg cggtcgagat tcctggaggc gggattgcgg tgctcggtgc
     1741 ggcggcggtc ctcaggcggc cgctttccgg agtgagtagg catcgctgga attatattgt
     1801 gcgaaggaat gcggaaccaa attggcggac ggatggtgtg aagattgacg cgagagcgtt
     1861 actagttcaa gagcaaccgg gcgaagtcat cgagtgccgt gtggggggaa tgggctatgc
     1921 tagggctaag tgtggttact tttggcccga ggctttgtgt cgggcaaacg agggcagcgg
     1981 ctagtctatg gatgtgctga agaagctttt cgaggagcgt tttggtgtac cggcggaacg
     2041 ggtgcagccg ctgcagggcg aattgggagc gtccgggcga aaaatttttc gcttgtgcgc
     2101 cggaaagacg cgcgcgatcg gaattttgta cggtgtgcgt gaagagaacc ttgcgttcct
     2161 tgcattttcg cgacattttc ggcgccatgg gttgccggtg ccggagattt acggagagga
     2221 tctggagcgc ggggcctatc tcgaagagga cctgggcgac acgaccctgt ttgagttttt
     2281 atcgaagcac cgcgacgggg aaaacgtaac gccggaagcg atcgaagcgt atcgcaaggt
     2341 ggtggaagct ctgccgcgtt ttcaggtgga ggcgggacgc gatctggatt attcggtgtg
     2401 ctatccatgc gcgagtttcg accggcaatc catcagctgg gacatcaact actttaagta
     2461 ttattttttg cggctggcgg ggattccgtt taacgagcag gcgctagaga acgatttctg
     2521 gcggttaacg gaatttttgc tgagcgcgaa ctgcgattac tttttgtaca gggattttca
     2581 gtcgcgcaac gtgatgctgc ctgagggcca accgtatttt ctggactacc agggcgggcg
     2641 caaaggggcg ttgcagtacg acattgcttc gctgctgtat gacgcgaagg ccgatttacc
     2701 gccggacgtg cgacaacagt tactggatca ttatctggat gcgctgcaag aacacgtaca
     2761 aatcagtcgc gaagagttct tgcaccatta ctacggttac gtgtatgtgc ggatcatgca
     2821 agcgatgggg gcgtatggct ttcgcggatt ttacgaacgc aaggcgcact ttttgcagag
     2881 cgtgccgtat gcactgaaga acgtgcgctg gttgctgcac aacgtgacgc ttgcggtgcc
     2941 gttgccgacg ctgatggaca cgttccggaa catgctggcg tcggaaaagc tgaacagctt
     3001 ggcgtcggaa gccgatgacc tggtggtgag aatcttcagt ttttcgtttc acaagggtat
     3061 gcccaaggat gaatcgggaa acggcggagg attcattttt gacgggcgga gcctgccgaa
     3121 tcccggaagg gaagaacgat tcaagcaatt gaccggcaaa gatgggcc
//
LOCUS       DASTPM010000703         8045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_948052, whole genome shotgun sequence.
ACCESSION   DASTPM010000703 DASTPM010000000
VERSION     DASTPM010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948052"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFR42_12465"
     CDS             complement(<1..487)
                     /locus_tag="VFR42_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370022.1"
                     /translation="MYSRAYKLLISVPPILWVALFLLFPYGLMFAHSFWSVSDGVILH
                     NWNLSNYRTLIHNPLYWQVLLRTARIAGSVTILSVLLGYPLAYFLSFHAGPRKELLYQ
                     LVIVPLWVSYLVRGYAWKTILGSDGVLNGFLQYVHITKEPVSALLYSPFAVILMLTHI
                     YT"
     gene            complement(547..1620)
                     /locus_tag="VFR42_12470"
     CDS             complement(547..1620)
                     /locus_tag="VFR42_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0370023.1"
                     /translation="MTSTAPIAPSVAPGNTLLEIRAVSKRFGPREVLKNLSLEIASGE
                     FLTLLGESGSGKTTLLRLIAGFDQPTAGEIWMHGTRLDTLPPYHRRVNTVFQQYALFP
                     HLTVSENVAYGLRAAAAPKPEIGARVHDALAMVRMQEYAAAKPARLSGGQQQRVALAR
                     ALVNRPELLLLDEPLSALDANLRKEMQSELKSLQRGVGITFLFITHDQEEAMALSDRI
                     ALLRNGGLEQVASPREIYARPATAYTAQFIGHTNLLRAVIREGVADCGGFYFPSALPE
                     GSALFSLRPEAIDLFANAPHTAHAVRFTASIRQQIYAGATELLELETDSGAILRARIA
                     ASAPLTGTHEFVFSASDAIPVKD"
     gene            complement(1617..2675)
                     /locus_tag="VFR42_12475"
     CDS             complement(1617..2675)
                     /locus_tag="VFR42_12475"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0370024.1"
                     /translation="MKRAPFLAILLVTAFVGSCKKSEPTLSLLVWEGYADPSFIHDFE
                     VSHHCKVVASYMGSSDELVAKLRGGSASNYDVISPSSDVATSIVKAGLVAPLDVVKIP
                     GYPQLSAKLRDSSLVKANGQVYGIPFMWGPNPLLYDTTAFAETPDSWAVLWNPKYRGR
                     VSLWDELSSIYMAAQILGYDRPDPNQLYNLSDEQLADAKKKLLELKPNVRKYWTTGGE
                     LTNLFQNHEIVLAMGWPLNTAQLRKLNFPIGETIPTENTTGWIDHLMITAASSRKDLA
                     TEFLAYMIEAKTQKLITDVTRYTPANPGAAQFMTDDEKKLLHLDNPDAYMQRIYFWQD
                     VPRRAKYNEIWNEVKAAQ"
     gene            complement(2768..3598)
                     /locus_tag="VFR42_12480"
     CDS             complement(2768..3598)
                     /locus_tag="VFR42_12480"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0370025.1"
                     /translation="MSSGKNLSRQSNSPPAGGALRISEVAKRVGISSSALRAWEALGL
                     VTPQRTQSRYRLYKEEDVRLLQRALFLRRARGLNPAAIVHILKRQGVVSVPAENTISP
                     GARFRRLRQRRGLSLAQVAKATGVSVGFLSALERGQMSASIATLRRIARYYRTNILSF
                     FEPAKDNSRLVKPAGRKILETTKGVRMELLAWGNTAMEPHLFRVKPGGNSGESYTHEG
                     EEFLHVLRGQFEIWLNQDEHYLLEPDDSLYFQSSTPHRWKNPGKKEAWVLWINTPPTF
                     "
     gene            3708..7565
                     /locus_tag="VFR42_12485"
     CDS             3708..7565
                     /locus_tag="VFR42_12485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU0370026.1"
                     /translation="MKSKRSFHTLLWNGMLYVSFFTLIATFCSSALFAQSTGGRIRGT
                     VTDQSGATVAAAKVIITNQANGTQRDTESGANGEYIFLEVPVGTYDIEVNQTGFKKYV
                     RKGVALDLNQIVSVDMALQVGTASETIEVTGAPPLVDTTTTQLGAVVNERTVSQLPLA
                     QRDAYQLLQLQPGVQSQVGLDTVYGSDRAGVVSVNGGRGRDNNFTVNGGDGNDQFAGL
                     PAIQPSPDAIAEFRVLTNTFDAEYGRNSGAVVNVVTKSGTNDIHGSLYEFFRNQSLNA
                     RGFFDTSKLDYLQNQFGATLGGPIKKDKTFFFVTYEGDRIRRGTSGDVVAVPTDAERA
                     GDFSGGGSFGGILANANILNNRPGCLAALGRNSAIADGTAYSDIFPAAQVPTACMDQT
                     ALDLLNQFVPHANAGDGLFQGVPLGHEHSDQVTLKIDHELTKNQRLTGYYYLENHDLA
                     KPFARFQSGGANIPGFGDLTDERIQQVNISHSWTIGATAVNEARFTFFREGQGTFLHP
                     QHTNLVQDSCVTVATANCFADPNNPDLGIHPNLGAQREGVPFVNISGGFNLGNNFEGE
                     LPQTGDTFQWSDNFSKTIGHHDLKFGGDVRYQRFDQTLFFDISGQYFYFGGGPNDPCL
                     GGSINSGTGACSGQFDLFANYLLGVPDEYGQGSAQEELVRSKSVYLFAQDSWKIKPSL
                     TLNYGLRWELNTPLADAGKKVQTFRPGQFSTVYPCALPAGSPLGPDCNTAGVTPVGLV
                     VPGDAGVPNGLSNTYYKSFAPRIGLAWSPSKKDGFLGKLFGGPGKSTIRAGFGVFYNP
                     VEQLVLEQFSAEPPFGGSTFVFNTQFNTPFLGQDGVTTFPNPFNGILNPPRGQPVDWS
                     LFRPILLFGQAAKNPRTQYAEQYNLTIQREVAKNMVATISYVGRQGHRLLASQDLNPG
                     NPTTCLDLIKLGEGCGPFLADSQYSFTLRAGETLHLPYIGGGPNGTNIPCPIAYPPAT
                     NYPAQCIVTGAAATPANPTGGTPINLVGIRPYSSPLCDPLTGNGCPNDGVAVFSDIFT
                     QNTIGNSAYNGFQASLEKRFSNGLQFTAAYTLGKSIDNSSTFENLVDPIDPRRNRSLS
                     LFDARNRFVFSYYWELPVPKFEGFKGKALNGWAVSGITTFQSGFPIRITEQDDIELQT
                     SFDFETPGQPNVAAPFHSINPRKLTCAVGTGTDPSTCTLINAGFNPDAFTVDTVSPGT
                     IGNAPRTICCGPGINNWEIGFTKFTPLTERFKLEFRGELFNAFNHAQFFQPDGNITDG
                     TDFGRVKRARDPRLVQFALKLSF"
     gene            7725..>8045
                     /locus_tag="VFR42_12490"
     CDS             7725..>8045
                     /locus_tag="VFR42_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0370027.1"
                     /translation="MSYEALEKELALYQKRTPKSAAAHKRAEQRIPLGVASNYRHYEP
                     YPIFVKDGRGSKIHDVDGNEYIDHNLCFGALMAGHCHPAVVKAVETKLHEGTAFGMPH
                     DQEWE"
BASE COUNT         1750 a         2210 c         2369 g         1716 t
ORIGIN      
        1 gcgtgtagat gtgcgtgagc atgaggatga cggcaaacgg gctgtagagg agagctgaga
       61 cgggctcctt cgtaatgtgg acatattgca agaagccgtt gaggactccg tcactgccga
      121 ggattgtctt ccaagcgtag ccgcgcacca gataactgac ccacagcggg acgatcacga
      181 gctgatagag aagttctttg cgcgggccgg cgtggaacga gagaaagtaa gccagtggat
      241 agccgagaag cacagaaaga atggtgacgg atccggcgat gcgggcggtg cgaagcagga
      301 cttgccagta taaggggttg tggatgagcg tgcggtagtt gctgagattc cagttgtgga
      361 ggatgacgcc gtcgctgaca gaccagaaac tgtgcgcgaa catcaaccca tagggaaaaa
      421 gcagaaagag cgccacccac agaatcggcg gcacggatat gagaagtttg taagcgcgcg
      481 agtacatgag tcagtgatcc ttcgctggcg cttggcgtcc ggtgattgga atgcgctgaa
      541 gcatgctcaa tccttcaccg ggatagcgtc agatgctgag aaaacaaatt cgtgagtgcc
      601 tgtgagtggc gcgcttgcgg cgatgcgggc acgaaggatc gcgccggaat cggtttccag
      661 ttcgaggagt tctgtggcgc ctgcgtagat ctgttgccgg atggacgcag tgaagcgaac
      721 ggcatgcgcg gtgtgcggcg cgtttgcgaa aagatcgatg gcctcggggc gaagagagaa
      781 tagcgccgaa ccttcaggga gcgcggaagg aaaataaaag ccgccgcagt cggcaacccc
      841 ctcacggatg acggcgcgca atagattggt atgcccgata aattgggcgg tatacgcggt
      901 ggctgggcgc gcgtagatct cgcgaggcga agcgacctgt tcgagtccgc cgttgcggag
      961 cagagcgatg cggtcggaaa gcgccatggc ttcctcctga tcgtgggtga tgaagaggaa
     1021 ggtaatgccg accccgcgtt gtagagactt cagttcggac tgcatttcct ttctcaggtt
     1081 cgcatcaagc gcggagagcg gttcgtccag caggagtagt tcgggacgat tcacgagtgc
     1141 gcgtgccaag gcgacgcgct gctgctgacc gccagagagc cttgccggtt ttgctgcggc
     1201 gtattcctgc atgcggacca ttgcgagcgc gtcatggaca cgggcgccga tttcgggttt
     1261 tggcgcagct gcggcgcgca ggccgtaagc gacattctcg ctgacagtca gatgcgggaa
     1321 gagcgcgtat tgttgaaaga cggtgttgac gcgccggtgg tagggaggaa gtgtatcgag
     1381 gcgcgtgcca tgcatccaga tctcgccagc cgttggctgg tcgaagccgg caatgaggcg
     1441 gagcaaagtg gttttgccgg agccgctttc gccaagcagc gtaagaaatt caccggaagc
     1501 gatttcgagt gagagatttt tcagaacttc gcgtgggccg aaacgtttgc tgacggcgcg
     1561 gatttccagc agcgtgttac ccggcgcgac cgaaggcgca atcggcgcgg tcgaagtcac
     1621 tgcgctgctt tgacctcgtt ccaaatttcg ttgtatttgg cgcggcgcgg cacatcctgc
     1681 cagaaataaa tgcgctgcat gtaagcgtcg gggttgtcca gatgcagaag tttcttttcg
     1741 tcgtcggtca tgaattgcgc ggcgccggga ttcgccggcg tgtagcgcgt cacgtcggta
     1801 atcagctttt gcgttttggc ttcaatcatg taggcgagga attctgtggc cagatctttg
     1861 cgtgagcttg ccgcggtgat catcagatgg tcaatccagc cggtggtatt ttccgtagga
     1921 atagtttcgc cgatgggaaa attcagtttg cggagttgcg cggtgttcaa cggccatccc
     1981 atagctaaaa cgatttcgtg attctgaaac aaattggtca gctcaccgcc ggtcgtccag
     2041 tatttccgga cattcggttt caactcgagc agctttttct tggcgtccgc gagctgttcg
     2101 tcggacaaat tatagagctg gttcgggtcg ggtctgtcgt aacccaatat ctgcgcggcc
     2161 atatagatgc tggagagctc gtcccacagg gaaactctgc cgcgatattt tggattccac
     2221 agaacggccc aggagtcagg cgtttccgca aacgctgtgg tgtcgtaaag cagaggattc
     2281 ggaccccaca tgaacggaat gccgtaaacc tgtccattgg ctttgaccaa tgaagaatcg
     2341 cggagtttgg cagagagttg gggatagcca ggaatcttca caacgtcaag cggcgcgacg
     2401 agaccggctt tcacaatcga agtggccaca tcgctcgacg gagaaatgac gtcgtagttc
     2461 gaagcgctcc cgccacgcag tttggcgaca agctcatcgc tcgagcccat gtaggacgcc
     2521 acgaccttac agtgatgcga aacttcgaaa tcgtggataa acgaaggatc ggcgtaccct
     2581 tcccagacca gcaggctaag cgtgggctcg gacttcttgc aggaacctac gaaggcagtc
     2641 acgagcagga ttgcgagaaa cggagcgcgc ttcatcaagc cctcccccaa tgcaaaccgt
     2701 ttgcgccggc attcgacagt atcaaatccg agctgacacc tgcaccggat agcaatgcga
     2761 cgcaatccta aaatgtcggc ggcgtattaa tccacaacac ccaggcttcc ttcttgccgg
     2821 gatttttcca gcgatggggc gtggaactct ggaaatagag gctgtcgtcg ggctcgagca
     2881 gatagtgttc atcctgattg agccagattt cgaactggcc gcgcagcacg tgcaaaaatt
     2941 cttccccttc gtgcgtatag gattcgccgc tgttcccgcc cggcttgacg cgaaaaagat
     3001 gcggctccat ggcggtgttg ccccaggcca gcagttccat gcgcacgcct ttggtggttt
     3061 ccaggatttt gcgccctgcg ggcttaacca accgcgaatt gtcctttgcc ggctcaaaaa
     3121 acgaaaggat gtttgtgcgg tagtagcgcg caatccgtcg caatgtggcg atggacgcgc
     3181 tcatctgtcc gcgttccagt gcgctgagaa aaccgaccga cacgcccgta gctttagcga
     3241 cttgcgcgag ggaaagtccg cggcgttgcc gcaatcgccg gaaacgcgcg ccgggcgaga
     3301 tcgtgttctc ggccggaacc gaaaccacgc cttgccgctt caagatgtgc acgatggccg
     3361 cggggttcaa tccgcgtgcc cgccgcagaa acagcgctcg ttgcaacagc cgcacgtctt
     3421 cctctttgta caggcggtag cggctttgcg tgcgttgcgg tgtgaccagt ccgagcgcct
     3481 cccacgcgcg cagcgcggac gaggaaatac caacgcgttt ggcgacttcg ctgatgcgca
     3541 gcgcaccgcc ggctggcggc gaatttgatt gtctcgataa attctttccg cttgacattg
     3601 actatcgctt tccctaagtt tacaggatta tagtaaggtt cacggggcac attcaacctc
     3661 gaagtctcgg tgcaaattct gaacttcgcg gttcaggggt gagttcaatg aaatcgaagc
     3721 ggtctttcca caccttgttg tggaacggta tgctgtacgt gtccttcttt accttgatcg
     3781 caactttctg ttcttccgct ctctttgcgc aaagcactgg tggccgcatt cgcggcacgg
     3841 taaccgacca gagcggcgcc accgttgcgg ctgccaaagt gatcattaca aatcaggcga
     3901 acggaacgca gcgcgatacg gaaagcggcg ccaatggtga gtacatcttt ttggaagtgc
     3961 cggtaggaac ttacgacatc gaagtcaatc agacgggttt caagaagtat gtgcgcaagg
     4021 gagtggctct cgatctgaac cagatcgtca gtgtcgacat ggcgctgcaa gttggcaccg
     4081 catcagaaac tattgaggtt acaggcgcgc ctcctctggt cgacactaca accacccagc
     4141 tgggcgcggt ggttaacgaa cgcacggtga gtcagttgcc gctggcgcag cgggacgcgt
     4201 accaactgtt gcagttgcag cccggcgttc aatcgcaagt tgggttggac acagtgtatg
     4261 gaagcgatcg ggcaggtgtg gtgagcgtga acggcggtcg cgggcgcgac aacaacttca
     4321 cggtgaacgg cggcgacggg aacgaccaat tcgcgggttt gccggccatt caaccttcgc
     4381 ctgacgcgat cgctgaattt cgcgtgttga ccaatacctt cgatgcagag tacgggcgca
     4441 attcgggcgc agtggtcaac gtcgtgacca agtctggaac gaacgacatt cacggcagtt
     4501 tgtacgagtt tttccggaac cagagtctga acgcgagagg ctttttcgat acctcgaagc
     4561 tggactacct tcagaaccag ttcggcgcga ctctcggcgg tccgattaag aaggacaaga
     4621 ccttcttctt cgtcacctat gagggcgatc ggattaggcg aggtacctcc ggtgacgtcg
     4681 tcgccgtgcc gaccgatgcc gagcgcgccg gcgacttttc gggaggcggc agctttggcg
     4741 ggattctggc caacgcgaac atactgaaca accgtccagg ctgccttgca gcgctcgggc
     4801 gcaactcggc tatcgccgat gggacggctt actccgacat ttttccagcc gctcaggtcc
     4861 ccacggcctg catggaccaa actgcattgg acttgctgaa ccaatttgtg ccgcacgcga
     4921 atgccggcga cggactgttc cagggagtgc cgcttggaca cgaacatagc gatcaggtaa
     4981 ctctaaaaat cgatcatgaa ttgacgaaga accagcggct taccggctac tactatctgg
     5041 aaaaccacga tttggcaaag ccatttgctc gcttccaatc gggcggagcg aatataccgg
     5101 gttttggaga cctgaccgac gaacgcatac agcaggtgaa tatttcccac tcctggacaa
     5161 tcggcgctac cgccgtgaat gaagcgcgct ttacgttctt ccgcgaagga caagggacgt
     5221 tcctgcatcc gcagcacacc aatctcgtgc aagattcctg cgtgactgtg gcaacggcaa
     5281 attgcttcgc cgatccgaac aatcctgatc tcggtattca ccctaacctc ggagcgcagc
     5341 gggaaggcgt tccctttgtc aatatatccg gcgggttcaa cctggggaat aactttgagg
     5401 gtgaactccc gcaaaccggc gacacatttc agtggtcgga taatttcagc aagaccatcg
     5461 gccaccacga cctgaagttc ggtggagacg ttcgatatca gaggttcgac cagacgcttt
     5521 ttttcgacat tagcggacaa tatttctatt ttggtggcgg accgaatgat ccctgccttg
     5581 gcggttccat caattccggc actggggctt gctccggtca gttcgacctc ttcgcgaatt
     5641 atctgctcgg cgttcccgac gaatacggcc agggttctgc gcaggaggaa ttggtgcgca
     5701 gcaagtcagt ctatctgttc gctcaggaca gctggaagat aaagccgtcc ctgactctca
     5761 actacggctt gcgctgggag ctgaatacac cgctggcaga cgctggcaaa aaagtacaga
     5821 catttcggcc cggtcagttt tcgaccgttt atccctgtgc gcttccggcg ggaagcccgc
     5881 ttggcccgga ctgtaatact gccggcgtga cccccgttgg tctggttgta cctggagacg
     5941 cgggagttcc caacggtctt tccaacacgt attacaaatc gttcgcgccg cgcatcggct
     6001 tggcttggag cccgagcaaa aaagacggct tcttgggtaa actgttcggc ggcccgggca
     6061 aaagtacgat tcgcgcgggc ttcggtgtct tctacaatcc cgtggagcag ttggtcctcg
     6121 aacagttcag cgccgaacct ccgtttggcg ggagcacatt cgtcttcaac acgcagttca
     6181 acactccgtt tctcggtcag gacggcgtaa ccacttttcc caacccgttc aacggaattc
     6241 tgaatccgcc tcgtgggcag cctgtggatt ggtccctgtt ccggcccatt ctgcttttcg
     6301 ggcaggctgc gaaaaatccg aggacccagt atgcggagca atacaacctg accattcagc
     6361 gcgaagtggc gaagaatatg gtggctacca tttcctacgt gggcaggcaa ggccatcgcc
     6421 tcttagcttc gcaggacttg aacccgggta atccgacaac ttgcttagac ctgatcaagt
     6481 tgggcgaggg gtgcgggcca tttttggcgg atagccaata ttcgttcacg cttagggcag
     6541 gtgaaactct tcatctgccc tatatcgggg gcggacccaa cggcaccaac attccatgtc
     6601 caatcgccta tccgccggca acgaactatc ctgctcagtg catcgtgact ggcgcggccg
     6661 ccacaccggc taatccaacc ggtgggactc cgatcaacct cgttggaatt cgaccgtact
     6721 catcgcccct gtgcgatccg ttaacgggga atgggtgccc caacgatggc gtggccgttt
     6781 tctctgacat cttcactcag aacacgatcg gcaattcggc atacaacggc ttccaggcca
     6841 gcttggagaa gcgcttttcg aatgggcttc agttcacagc cgcttacacg ctcggcaaat
     6901 ccatcgacaa ctcttcgacc tttgaaaacc tcgtggaccc aatcgatccg cggcgcaatc
     6961 gatctctatc gctctttgat gcgcgtaacc gtttcgtgtt cagctactac tgggagttgc
     7021 ctgtgccgaa attcgaaggt ttcaagggta aggcgctcaa tggatgggcc gtctcgggaa
     7081 tcacgacgtt ccagtccgga ttccccattc gtattaccga acaagatgac atcgaattgc
     7141 aaaccagctt cgacttcgag acgcctggtc agcctaacgt agcagctccg ttccattcga
     7201 ttaacccgcg caagctgact tgcgccgtgg gaacgggcac cgacccttct acctgcacgc
     7261 tcattaacgc cggcttcaat ccagacgctt tcacggtaga cacggtttcg cccggcacga
     7321 tcgggaacgc gccgcgaacg atttgctgcg gtcctggcat taacaactgg gagatcgggt
     7381 tcactaagtt cacgccttta acggaacgct ttaagctgga attccgcggg gagcttttta
     7441 acgcctttaa ccatgcgcag ttcttccagc ctgatggcaa cattaccgac gggacggatt
     7501 tcgggcgcgt gaaacgcgct cgcgatccgc gcctagtgca attcgcgttg aagctctcct
     7561 tctaagggga gagcatgttg aggaccgcca gcgcggctca cgagggccgc gcttccgcgc
     7621 gtctgaaaag gcgtcatgat tttcgcagca cactgacggc agggaagaga cctgcaagga
     7681 gcaggctccg cgcgagcgga gcaagcttga ttcggagaaa taccatgtcg tacgaagcac
     7741 tcgaaaaaga gctggcgctc tatcaaaagc gcaccccgaa gtcagccgcc gcccataagc
     7801 gcgctgagca acgcatcccg cttggcgtgg ccagcaacta tcgtcactac gagccgtatc
     7861 cgattttcgt gaaggacggc aggggcagca agatccatga cgttgacggc aacgaataca
     7921 ttgatcacaa cctttgcttc ggcgcgctca tggccggcca ctgccatccg gccgtcgtca
     7981 aagccgtcga aaccaaactg cacgaaggca ctgcgttcgg catgccgcat gaccaggaat
     8041 gggaa
//
LOCUS       DASTPM010000704         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1409648, whole genome shotgun sequence.
ACCESSION   DASTPM010000704 DASTPM010000000
VERSION     DASTPM010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409648"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1610
                     /locus_tag="VFR42_12495"
     CDS             225..1610
                     /locus_tag="VFR42_12495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0370028.1"
                     /translation="MTRFPTALEEIGQAQLLARASKHDSVDRNARFIALNTLLRANIE
                     GVNNDLKVAQRGGAEDRLKQAVEPALAAFATKATAYLDALRASIGAGGANVTRPDAEY
                     ASAVRSALDAWKTTQQQLDQLLIQRIDDLQRQRLLSLVVIGTLGMLGLFVAFLTYRDM
                     IVPIRQLADLANNIRETKNYGLRSDYKSRDEIGGLAASFNEMLGELEVAREREMMRQA
                     EISRVSRLTTMGAMVASIAHEIRQPLAALVANSHAGTRWLERKEPNLEEARSALASIA
                     QDGHRANEVITSISMMFKKNANRRVPVDTNEVVNDVINLSRGELRSRKIALHTKLAPD
                     LPQVPADPVQLREVLLNLIMNAAEAMNSTDESRRVLAIVSMPADDGISITVEDSGIGL
                     DPKNAEQIFEAFFTTKPTGMGMGLAICRSIIAAHGGRLWASPGHSCGTVFHVVLPLRT
                     GQRASAEAPTI"
     gene            complement(1632..1955)
                     /locus_tag="VFR42_12500"
     CDS             complement(1632..1955)
                     /locus_tag="VFR42_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370029.1"
                     /translation="MRAALAGLGGVLLLALLSRADAQDIRGLEVCTAEKQMERRTGCL
                     QANVEFLQQALAKLARETQEKIASAGRDLAAARAEIAALKATVAKLDSELAQLKAKAE
                     PANKK"
     gene            2103..>2852
                     /locus_tag="VFR42_12505"
     CDS             2103..>2852
                     /locus_tag="VFR42_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0370030.1"
                     /translation="MKQLRDLRARGRRSDRLRSITAFLMLALSMLAVQPASAANGQVL
                     KAVKDRGTLNCGVSEGLYGFSARDNNGNWSGFDVDLCRAIAAAIFNDISKVAYFPLEA
                     GRRFEALQSGSIDVLSRNTTWTMSREVELGLVFAATNFYDGQGFIVRRAMNKDTALDL
                     DGMKVCVQTGTTTVLNLADYFRANKMKYEAISSTSLEGAIKGYDSGQCNVLTADGSAL
                     FAVRLLLSKPDEHIILPDVISKEPLGPVVRQD"
BASE COUNT          610 a          840 c          809 g          593 t
ORIGIN      
        1 tggcgcgggc agatgccgaa acctccggca ctttgcacat agcaaaagcg gcagaggcgc
       61 tagcgaccac tctacgcggt ctggaaacca gcaattctgc gagcacggac gacatttatt
      121 ctgctgctct caaaactctc cgcgatctcg ccgctcgtat aggagacgat tcaaacataa
      181 ttcttgatcc cgttcttgac acttaccacg tcggaaacat cgttgtgacg cgttttccga
      241 cagcgctgga agagattggc caggcgcagt tgcttgcacg agcgtcgaaa cacgactctg
      301 ttgaccgcaa tgctcgtttt atcgcactca acacccttct gcgcgcgaac atagaaggcg
      361 tcaacaacga tctaaaagtc gcgcaacgcg ggggcgctga ggaccgtttg aagcaagccg
      421 tcgaacccgc tctggcagct tttgcgacaa aagcaacggc atatctcgat gcgttgcgcg
      481 cctccatcgg tgctggcgga gcaaacgtca cgcggccgga cgccgagtat gccagcgcag
      541 tcagaagcgc tctcgatgcc tggaaaacaa cgcagcaaca actcgaccag ctgttgatcc
      601 agcgaattga tgatctgcaa cggcaacgtc ttttaagtct tgtggtgatc ggaacgcttg
      661 gaatgcttgg ccttttcgtc gcctttttga cttatcggga tatgatcgtt ccgatcaggc
      721 agctggccga tttggccaat aatattcgtg agaccaaaaa ctacgggctc cgttctgact
      781 ataaaagtcg agacgaaata ggcgggctgg cagcttcatt taacgaaatg ctcggtgaac
      841 tcgaggttgc gcgcgagcgc gaaatgatgc gtcaggccga aatatcccgc gtgtcgcggc
      901 tcacgaccat gggcgccatg gttgcttcca tcgcgcacga aatcagacag ccgttggcgg
      961 cccttgtggc caattcgcac gccggaaccc gttggctcga aagaaaggaa cccaatctag
     1021 aggaggcgcg ctcggcgttg gcaagcatcg ctcaggatgg ccaccgcgcc aacgaagtca
     1081 tcacaagcat cagcatgatg ttcaaaaaga atgccaaccg tcgcgttcca gtcgacacca
     1141 acgaagtcgt aaacgatgtc atcaatctct ctcgcggcga actccgctcg cgaaagatcg
     1201 ccctgcatac aaagctagcg cctgaccttc cacaagttcc tgcagacccg gtccagctcc
     1261 gcgaagtgct cctcaatctg atcatgaacg cggccgaggc catgaactcg accgacgaaa
     1321 gccgacgtgt cctggcgatc gtatcaatgc ctgccgacga tggcatttcg attaccgttg
     1381 aagattccgg cattgggcta gaccccaaga acgccgagca aattttcgaa gcattcttca
     1441 ccaccaaacc gacaggaatg ggaatgggcc tcgcgatttg ccgctcgatt attgcggccc
     1501 atggcggacg cctgtgggca tcgccgggac attcatgcgg cacggtattt cacgttgttt
     1561 tgccactcag aacgggtcag cgggcctccg ccgaagcgcc gactatctga gctgcccact
     1621 aacaaccctg tctacttctt gttggcgggt tcggccttgg ccttcaattg cgccagctcg
     1681 ctgtcgagtt tcgcaaccgt ggctttaagc gctgcgattt cggctcgcgc ggcggcgaga
     1741 tcgcgcccgg cgctggcgat cttctcctgc gtctcgcgcg caagcttcgc gagcgcttgc
     1801 tgaagaaact cgacgttggc ctgcaggcac ccggtgcggc gctccatctg cttttcggcc
     1861 gtgcagactt ccagcccacg gatgtcctgt gcatctgccc gcgacagcag cgcgagaaga
     1921 agcaccccgc ccaagcctgc aagcgccgcg cgcaagaacc gctggtgttc aaaagtcatg
     1981 cttgagcagc ctgttcaagt cggaggatcg gggaatgcag ggttgatccg cgcggccact
     2041 tataccagcc tcgtttcaat atgctagctt agctacaggt ctgtcgagcc tcgggggaag
     2101 tcatgaagca gttgcgggat cttcgcgccc gtgggcggcg tagcgatcgc cttcggtcga
     2161 ttacggcatt tctgatgctc gcgctgtcga tgctggcggt ccagcctgcc tccgcggcga
     2221 acggccaggt actgaaggcc gtgaaggatc gcggcacgct caattgcggc gtcagtgaag
     2281 gcctttatgg attttccgct cgcgacaaca acgggaactg gagcggcttt gacgtcgatc
     2341 tgtgccgggc gatcgcggcg gcaattttta atgacatcag caaggtcgcc tacttcccgc
     2401 tcgaggccgg ccggcgattc gaggctctgc aatccgggag catcgacgtg ctctcgcgca
     2461 ataccacctg gaccatgtcg cgcgaggtcg agcttgggtt ggtcttcgcg gcgacgaatt
     2521 tctacgacgg ccagggattt atcgttcgcc gcgctatgaa caaggacacc gcgctcgacc
     2581 tcgatggcat gaaagtatgc gtgcagacgg gaacgacaac cgtgctcaat ctcgcggatt
     2641 atttccgcgc caataaaatg aaatacgagg cgatttcttc gacaagtctc gagggcgcca
     2701 tcaagggcta cgattccggc caatgcaacg tgctcacagc tgacggatcc gcgctgtttg
     2761 ctgtgcggct tctgctgtcg aagcccgacg agcatatcat tctgccggat gtgatctcga
     2821 aagagcccct gggtccggtt gttcgacaag at
//
LOCUS       DASTPM010000705         3381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_255922, whole genome shotgun sequence.
ACCESSION   DASTPM010000705 DASTPM010000000
VERSION     DASTPM010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255922"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFR42_12510"
     CDS             <1..490
                     /locus_tag="VFR42_12510"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU0370031.1"
                     /translation="GQFRGYLSNRETAKLIGLNRSSGTMRAESWNRLPIIRMTNVSLR
                     PRQGSPHDLISSTDDGIWMETNRSWSIDDRRYQFQFSTEIAWEIKGGKKVRMLKNPSY
                     SGITTEFWNSCDGICGKEFWTLWGTPNCGKGQPMQTMGTGHGAAPARFLNVKMGTAFA
                     GH"
     gene            494..1873
                     /locus_tag="VFR42_12515"
     CDS             494..1873
                     /locus_tag="VFR42_12515"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU0370032.1"
                     /translation="MKKSSKSGSAGALSSAAELKKIIDVVLRLGKAAGAYETEVHFDE
                     TIEALTRFANNAIHQNVSEHGITVSVRTVVDGRTARASTNRLDEDSLRRAVESSLQLA
                     HSQPKNRNLLPMSGKQRYRILNRFSRQTAALSAEARARAVKQASDLAIRNGQVAAGIF
                     SSGQSQLALGNSRGLFATHRQTHAEFSITMQNDPAASWAKANSGDVRKIDPQALAKRA
                     SEKASMAKDAKELASGKYTVILEPAAVLDLVGFLFYDFAATALEDQRSCLNKRMGKQL
                     FGKNISIVDDVYHPFQLGAPFDGEGVPRQRVSLVEKGVPKNLVYSRASAKKAGKKPTG
                     HGFGLPNEYGEAPVNLVFGGGGSSVEKMVAGTERGLLVTRVWYIREVDPYEKVMTGMT
                     RDGLFLVENGKLTTAVRNFRFNQSLIEILQNVQEMGPAVRTTAEEAFEMVVPAMKVTG
                     FHFTEPTRF"
     gene            1979..>3381
                     /locus_tag="VFR42_12520"
     CDS             1979..>3381
                     /locus_tag="VFR42_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370033.1"
                     /translation="MDLPSHWRQWRSTDRVRPNWGHPDHDACGTGFIARIGGPAGHDI
                     IDYALTALERLTHRGGVDADGASGDGAGLLAALPTQFFRAKAAEQGIELPEQFGVGFA
                     FFPSESVAAAKAAIETAADQERLRVLGWRRVPTNPNCLGRRALETMPESWQFFVAPFH
                     IARHADRFEWRLAILRKKAESLLPARCYVCSLSSQTVVYKGLLTPWQFPQFFEDLRQP
                     SFETTFVIFHQRYSTNTQPAWSLAQPFRYVAHNGEINTIVSNRRWLRAKEKQLREKLT
                     VGSWFQALEPNVSDSASFDNALEVKLLGGFSAEESMLALVPPAFENDPLLSRDVKGAL
                     SALSQQSEPWDGPAALIFSDGQYVGAKLDRNGLRPMRYTLTHDGLLVVGSETGLVDFE
                     ESRIAERHRLGPGEMIIAKPESGLLLRWRDILKRLGGHQSKLTPPTVRRLDTSVTPPP
                     SAQVEAPRRTAAACGWT"
BASE COUNT          711 a         1102 c          885 g          683 t
ORIGIN      
        1 cgggcaattc cgcggttatc tctcgaatcg ggaaacggcc aagctgatcg gcctgaatcg
       61 ttcctcgggc accatgcgcg ccgaaagctg gaatcgcctg cccattattc gcatgacgaa
      121 tgtcagcctc agaccacgcc aaggatcacc tcacgatctc atctcttcca ccgatgatgg
      181 tatttggatg gagacgaacc gttcctggtc catcgacgat cgccgctatc aatttcagtt
      241 ctccacggaa atcgcttggg agattaaggg tggcaagaaa gtgcgcatgt taaaaaatcc
      301 cagctatagc ggaatcacca cggaattctg gaattcgtgc gatggcattt gcggcaaaga
      361 attctggacg ctatggggta cgccgaattg cggcaaaggc cagcctatgc aaaccatggg
      421 cacgggccat ggagcagcgc ccgcgcgttt tctgaacgtg aagatgggca cggcctttgc
      481 ggggcactga gaaatgaaaa agagttcgaa gagcggatcg gccggcgcac tgtcgtcagc
      541 ggctgaactg aaaaagatca tcgacgttgt attgcgcctc ggcaaagcag ccggagccta
      601 tgaaaccgaa gttcatttcg acgaaacgat tgaagctttg acgcgtttcg cgaacaacgc
      661 tatccaccag aatgtttcgg aacacggaat cactgtttcg gtaaggaccg tggtcgatgg
      721 ccgcaccgct cgcgcttcca ccaaccggct cgacgaagat tccttgcgca gggccgtcga
      781 atcttctttg cagttggcgc acagccagcc caagaatcgc aacttgttgc cgatgtcggg
      841 caagcagcgc taccgaatct tgaaccgctt ctcacgccaa accgccgcgc taagtgccga
      901 agctcgcgct cgagccgtga aacaagccag cgatctggcc attcgtaacg ggcaggtggc
      961 cgccggaata ttttcgtcag gccagtcaca actcgcactc ggcaactccc gcgggctatt
     1021 tgccacgcat cgccaaactc acgccgagtt ttccatcacc atgcagaatg atcccgcggc
     1081 cagttgggcc aaggccaact ccggcgacgt acgaaaaatt gatccgcaag cgcttgccaa
     1141 gcgcgccagc gaaaaggcca gcatggcaaa agatgccaag gaactcgctt ccggtaaata
     1201 caccgtgatt ctcgagccgg ccgctgtgct cgacctcgtc ggtttccttt tttacgattt
     1261 cgccgccact gctctcgaag atcagcgttc ttgcctcaac aaacgcatgg gcaaacagct
     1321 cttcggcaaa aacatctcca tcgtggacga tgtctaccat ccgttccagc ttggcgctcc
     1381 atttgacggc gaaggcgtgc ctcgccagcg cgtttccttg gtggaaaaag gtgttcccaa
     1441 gaatctagtc tactcacgcg ccagcgcgaa aaaagccggc aagaaaccta ccggccacgg
     1501 cttcggcctg ccgaatgaat acggcgaagc gcccgtgaac ctcgttttcg gcggcggcgg
     1561 ttcttccgtc gaaaaaatgg tggccggcac cgaacgcggc ctgcttgtaa cccgtgtctg
     1621 gtacatccgc gaagttgacc cctacgaaaa agtcatgacc ggcatgaccc gcgacggcct
     1681 gtttctggtc gaaaatggta aactgaccac cgcggttcgc aacttccgct ttaaccaatc
     1741 tctcatcgag atattacaaa acgttcaaga aatggggccg gctgtccgta ccaccgccga
     1801 ggaagccttc gaaatggtcg ttcccgccat gaaagtcacc ggcttccact ttaccgaacc
     1861 caccagattc taaccttttt cgtcgctccc tccgccaaac ctcagtaatc agaaattcaa
     1921 tcttgaatat cgaatttctg cgttgtacag agtcctctcc ctacccttat aaacgaacat
     1981 ggacttgccg tcccattggc gccagtggcg gtcgactgat cgtgtgcgcc ccaactgggg
     2041 ccatccggac catgacgcct gcggcaccgg cttcatcgct cgcatcggcg ggcccgccgg
     2101 tcacgacatc atcgattacg ccctcaccgc cctcgaacgc ctgacccatc gcggcggcgt
     2161 cgatgccgac ggagccagcg gtgacggtgc cggccttctg gccgcgttac ccacgcagtt
     2221 tttcagagcg aaagccgccg agcagggcat cgagcttcct gagcagtttg gtgtaggctt
     2281 cgcctttttt ccgtccgagt cggtggccgc cgccaaagcc gccattgaaa ctgccgccga
     2341 tcaggagcgc ctccgcgttc tcggctggcg ccgcgtcccc accaatccca actgcctcgg
     2401 acgtcgtgcg ctcgaaacca tgccggagtc ctggcaattc tttgtcgcgc catttcacat
     2461 cgcgcggcac gccgatcgct tcgagtggcg cctggccatc ttgcgcaaga aagccgaatc
     2521 gcttcttccc gcgcgatgtt acgtctgttc gctttcctcg caaaccgtcg tgtacaaagg
     2581 cctcctgacc ccctggcaat ttccgcaatt cttcgaagac ctccggcagc cttcgttcga
     2641 aacgacgttc gttatttttc accagcgcta ctctaccaac acacagccgg cctggagcct
     2701 cgcgcagccc ttccgctatg tcgcgcacaa cggcgaaatt aacactatcg tttccaatcg
     2761 ccgctggctg cgcgccaaag agaagcaact ccgggagaaa ctcacggtcg gctcgtggtt
     2821 ccaggcgctg gagccaaacg ttagcgactc cgccagtttc gacaacgcgc tcgaggtcaa
     2881 acttctcggg ggtttctcgg ccgaagagtc catgctggct ctcgttcctc ccgcgtttga
     2941 aaacgatccg ctcctctcgc gcgacgtcaa gggtgctctg tccgcgctct ctcaacagag
     3001 cgaaccctgg gacggtcctg ccgccctgat tttttccgac ggccaatatg tcggcgcaaa
     3061 gctcgatcgt aacggtcttc gccccatgcg ctacaccctg acccacgacg gcctgctcgt
     3121 cgtcggctcc gaaaccggcc tcgtcgattt tgaagagtcg cgcattgccg aacgccaccg
     3181 cctcggccca ggcgaaatga tcatcgccaa gcccgaatcc ggcttgctgc ttcgctggcg
     3241 cgacatcctc aagcggctcg gcggccatca gtccaagctc acgcctccaa ctgtccgccg
     3301 cctcgacacg tccgtcacgc cgccgccctc cgcacaagtc gaagcgccgc gtcgcacggc
     3361 ggccgcctgc ggctggaccg a
//
LOCUS       DASTPM010000706         4639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1179209, whole genome shotgun sequence.
ACCESSION   DASTPM010000706 DASTPM010000000
VERSION     DASTPM010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179209"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFR42_12525"
     CDS             complement(<1..252)
                     /locus_tag="VFR42_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370034.1"
                     /translation="MRTRVGLALACSLACTAAFLLTAVPARTQSPAAEKPAVYTYVSE
                     WAVPRAMWADYKKEDDADLEAMKKAMADGTIVSSGRFAVL"
     gene            complement(389..1345)
                     /locus_tag="VFR42_12530"
     CDS             complement(389..1345)
                     /locus_tag="VFR42_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0370035.1"
                     /translation="MEQARFAEAREFLARYLPRTRLVAARSLQKDRKQKVFLKLESEL
                     PTGSFKVRGALYALHVNLQRHSIREVVASSTGNHGAAVAYAATELGVRARIFLPENCN
                     PVKKSNIERLGAEIVEKGAIDLAGAFEAAADYARKSGAFFLNDADDPDLPAGPGTIAC
                     EILQSAPEVDSIYVPMGDTALIRGVAESARQISGKVRVVGVQSERAPSYALSWKAGKA
                     VGTETCDTIADGLATRTPLSANVEAIRRLVDDVVLVSEAEMLRAIRQLALNEHVVAEP
                     AGAAATAAFLKSSSNANASVALLVTGANVSSEVFRAAIGVAT"
     gene            complement(1349..3562)
                     /locus_tag="VFR42_12535"
     CDS             complement(1349..3562)
                     /locus_tag="VFR42_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0370036.1"
                     /translation="MLRRIGSGGMGVVYEAEDTKLGRKVALKFLPAEMAQDTLTLERF
                     QREARAASALNHPNICTIHSIEQHERQHFIVMELLEGQTLGQRIGRQAFEMEKLLPLA
                     IQIADALESAHAKGIVHRDIKPANIFLTERGQVKILDFGLAKVEKISAVSESTSLSPF
                     ADTLGETGALTSPGSAVGTIMYMSPEQARGGLVDARTDLFSLGTVLYQMATGSLPFPG
                     DTSAVIFDAILNRQPLPVETVNGMVPREFGRLLEKLLEKDRNLRCQSATELKTDLLRL
                     KRDLESGKRIAAEVSDSKAGTNSRGPKSLAVLYFENLSGVKEDEYLRDGITEDIITEL
                     SKIRGLNTFSRPTVLAFRDKTVTPVQIGQQLKAAYVLTGTLRRAGNRLRITTQLVDTH
                     TDFPLWSERYDREMKDVFEVQDEIARKIAEALRVTLSPQELEALAIKPTENLQAYDLY
                     LRGKRYARRQTRQDLEFALQMFESAVAMDPNFALAYAACANACAMFYCNYSRDVSWVE
                     RAREASGKAVALRWELPEVQVSQAWVLYATELHDEAVRMVRKAIERKKDCEGAYYLLC
                     RALFSAGRYQEVVDVMEAGLEASGEDYNIYVPLANSLGAMGKREAYMNLLQRRVTALE
                     NHLKQVPEDARARILLGTDYAELGREEDALKELNLAVTLRAGEASILYNAACLYATLK
                     RKSEAMDCLKKAWEAGFRDASHARRDPDLALLHDDPEFDRMYPEKSASGVQGRVN"
     gene            3955..>4639
                     /locus_tag="VFR42_12540"
     CDS             3955..>4639
                     /locus_tag="VFR42_12540"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEU0370037.1"
                     /translation="MYSVWSGWADATQTRTVDFLTFWSVPQALNRQSIPNIYTLEGQR
                     TLASATVNLARSSSASDLQRRTTAAVLELYNGRIDTTASPLLYSLIGVLSSGNYLTDQ
                     KRFLFACMSAFVLSILVLARLLKFSLLETVLFLLFLFWNYEPFLSDIRVGNVNELQLF
                     ALVLFIFFMARSRPFSAGFIVGASTTFKPTALLVLVLAFIASVADKDYRSVLRLFLGS
                     FVSAVSLITL"
BASE COUNT          895 a         1543 c         1110 g         1091 t
ORIGIN      
        1 cagcacggcg aaacgcccgg aggaaacaat cgttccgtcc gccatcgctt tcttcatcgc
       61 ttccaggtcc gcgtcgtctt ctttcttata gtccgcccac atggctcgtg gaacggccca
      121 ttccgaaacg tacgtataca ccgccggctt ttccgccgca gggctctgcg tccttgccgg
      181 aactgccgta agcaaaaatg ccgctgtgca tgccaacgaa catgccaagg ctagacctac
      241 tcgtgttcgc attctttttc ctccaagatt tacctgcacc cgcggaaaaa tctggccgaa
      301 ttttgttttt gaacaagtcg aaggggaacc gattgctgac agacgctacg ccagcgactc
      361 gcgcgtgtca agctgatggg gcaaatcctt acgtcgcaac tccgatcgca gctcgaaata
      421 cttcagagct cacgttggct cccgtcacca agagcgccac tgacgcgttt gcattggaag
      481 aagatttaag aaatgccgca gtcgccgccg ctcctgctgg ctccgctacc acgtgctcat
      541 tcagcgccaa ttgccggatt gcgcggagca tctcggcttc gctgactaga accacgtcat
      601 caaccaggcg gcgaattgcc tcaacgttag cggacaaagg agtccgcgtc gccagcccat
      661 ctgcaatcgt gtcgcaagtt tccgttccca cggctttgcc cgccttccac gaaagcgcat
      721 acgaaggcgc tcgttcgctc tgtacgccga caacccttac tttgccggaa atctgcctgg
      781 ccgactccgc cacgcctcgg atcagcgccg tgtcccccat ggggacataa atcgaatcaa
      841 cctctggcgc actctgcagg atctcacagg caatggttcc cggacccgcc ggcaggtcag
      901 gatcatctgc atcatttaaa aagaatgcgc cgctctttcg cgcgtagtcc gccgccgctt
      961 cgaacgcacc cgcgagatcg atcgctcctt tctccacgat ctcagcgcca agccgctcga
     1021 tgtttgactt tttgacggga ttgcagtttt ccggcaagaa gattcgcgcc cggactccta
     1081 gctccgtcgc cgcatatgcc accgccgcgc catgattccc cgtgctcgat gcaaccactt
     1141 cccttatcga gtgccgttgc agattcacat gcagggcata cagtgccccg cgcaccttga
     1201 acgaaccggt aggcagctcg ctttccagct tcaagaaaac tttttgcttg cgatctttct
     1261 gcaacgagcg tgccgcaacc agccgggtcc gcggcaaata cctggccaaa aactcgcgcg
     1321 cctcggcaaa tcgcgcctgc tccatctctc aattcacccg cccttgcact ccactcgcgc
     1381 ttttctcggg atacatcctg tcgaactccg gatcgtcatg cagcagcgcc agatccggat
     1441 cgcgtcgcgc gtggctggcg tctcgaaacc ccgcttccca agccttcttg aggcaatcca
     1501 tcgcctcgga tttccttttc agcgtggcat acaggcacgc cgcgttgtac agaatcgaag
     1561 cttccccggc tctcagtgtc accgccagat tcaactcctt caatgcgtct tcctcgcgcc
     1621 ccagttcggc atagtcggtg cccagcaaaa ttcgcgctcg cgcatcttcc ggcacctgct
     1681 tcaggtgatt ctccagagcc gtcacgcgcc tttgcagcag attcatgtac gcttcacgct
     1741 tgcccatcgc gcccagcgaa ttcgccaggg gcacataaat gttgtagtcc tcaccgctcg
     1801 cctccagccc tgcttccatc acgtccacca cttcctggta gcggcccgct gaaaagagtg
     1861 cgcggcacag caggtaatac gcgccttcgc agtctttttt tcgttcgatc gctttgcgca
     1921 ccatacgcac cgcttcatcg tgcaattccg tcgcgtacag tacccaggcc tgactcacct
     1981 gcacttccgg taattcccaa cgcagcgcca ccgctttgcc ggaagcctcg cgcgcgcgct
     2041 ccacccaaga aacgtctcgg ctgtaattgc aataaaacat cgcacaggca ttcgcgcacg
     2101 ccgcgtaggc cagcgcaaaa ttcgggtcca tcgccacggc actctcaaac atctgcagcg
     2161 cgaactccag gtcctgccgc gtctggcgcc gggcataccg cttcccgcgc aggtacaagt
     2221 cataagcctg taggttttcc gtcggcttga tcgccagcgc ttccagttcc tgcggtgaca
     2281 gggttacgcg cagagcctcc gctatcttgc gcgcgatctc gtcctgcact tcgaacacgt
     2341 ccttcatctc gcggtcgtag cgctcgctcc acagcggaaa gtccgtgtgc gtgtccacca
     2401 gttgggtcgt gattcgcagc cggttcccgg cgcgtcgcag cgtcccggtc agcacatatg
     2461 cagctttcag ttgctgtccg atctgcacgg gagttaccgt tttgtcgcga aacgccaaca
     2521 ccgtcggtcg cgaaaaagta ttcagtccgc gaatcttcga cagctccgtg atgatgtctt
     2581 cggtgatccc gtcgcgcagg tattcgtctt ctttcactcc actcagattc tcaaaataaa
     2641 gcaccgccag cgacttcgga ccgcggctgt ttgttccggc tttcgaatcg ctcacttccg
     2701 ccgcgatccg cttgcccgac tccaggtccc gctttagccg cagcaaatcg gttttcagtt
     2761 ccgtagccga ctggcaccgc aaattccggt ccttctccag cagcttctcc aacaatcgcc
     2821 cgaactcgcg gggcaccatg ccatttaccg tctctactgg aagaggctgg cggttcaaaa
     2881 tcgcgtcaaa aatcaccgct gatgtgtctc ctggaaacgg caagctcccg gtcgccatct
     2941 ggtacaacac cgttcccagc gaaaacaaat ccgtccgcgc atccaccagc ccgccgcgcg
     3001 cctgctccgg cgacatgtac atgatcgttc cgaccgcaga gcccggcgaa gtgagcgccc
     3061 ctgtctcccc caacgtatcg gcgaacggcg agagcgatgt actctccgaa accgcgctaa
     3121 ttttctcaac cttcgccaac ccgaaatcca gaatctttac ctgtcctcgc tccgtcagga
     3181 aaatattcgc cggcttgatg tcgcggtgca caattccttt cgcgtgcgcc gattccaggg
     3241 catcggcaat ctgaatcgcc aaaggtagca gcttttccat ttcgaacgct tgccggccga
     3301 tgcgctgacc cagtgtctgg ccttccagta actccatcac aataaaatgc tgccgctcgt
     3361 gctgttcgat cgaatgaatc gtgcagatgt tcggatgatt cagcgccgaa gctgcccgcg
     3421 cctctcgctg gaaccgctcc agcgtcagcg tgtcctgcgc catttcggcc ggcaagaatt
     3481 tcagcgctac cttccggccc aacttggtgt cttccgcttc atagaccaca cccatcccgc
     3541 cgctgccaat cctgcggagc accctgtaat gggaaaccgt ctggcctgct ggagactcct
     3601 gttcgggcat ggtgcgctaa atcctagccg ccacgctcaa cccggtcaag ccagttcagt
     3661 ctccactgcg attttgcacc agaatttcct gcctattagt caattcacag tacgtaactc
     3721 gttgaaattg acttgccgac cacttccact ttgcagggta acctgtttgc gtcataaaac
     3781 tgacgctggc tccgtttttg cggcggattc gagggaactt tggggtcgac agaaagctcc
     3841 gtggaaggtc catgcaaaaa cgccgacacc ggacttcgga accaaacccc tgaattcttg
     3901 gatcctcgta gctcgtccct tcgcactgtc ctcatcttga gctgcttact tttggtgtac
     3961 tccgtctggt ctggttgggc ggatgccaca caaacccgca ctgttgattt cctcacgttc
     4021 tggagcgttc cacaggcgtt gaacagacag tccatcccga atatctacac cctggaaggg
     4081 cagcgcacgc tggcttcggc cactgtgaat ctggcacgat cctcttctgc ttcggatctt
     4141 cagaggcgaa caacggccgc cgtcttggaa ctctacaatg gacgaatcga caccactgcc
     4201 tcgccgcttc tttattccct catcggagtc ctttcgtcag ggaattatct gacggaccag
     4261 aagaggtttt tgttcgcctg catgtcggct ttcgtgcttt ccatcctcgt gttggccagg
     4321 ctattgaagt tctctctact cgaaaccgta ctcttccttc tctttctttt ctggaattac
     4381 gaaccgttcc tttctgatat tcgagtcggc aatgtaaatg agcttcaact ttttgcactc
     4441 gtgctgttca tctttttcat ggcccgctcc cggccttttt cagccggatt tatcgtcgga
     4501 gcctccacga cgttcaaacc gaccgcgctc ctggttctcg ttctggcctt cattgcaagc
     4561 gtcgccgata aagactacag gagcgtacta cgcttgtttc ttggctcttt cgtatcggcc
     4621 gtctcgctga tcacactgt
//
LOCUS       DASTPM010000707         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1179212, whole genome shotgun sequence.
ACCESSION   DASTPM010000707 DASTPM010000000
VERSION     DASTPM010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179212"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..927)
                     /locus_tag="VFR42_12545"
     CDS             complement(25..927)
                     /locus_tag="VFR42_12545"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HEU0370038.1"
                     /translation="MYVVLGASGNTGSVVARKLLAKGEKVRAVGRDATKLSALARLGA
                     DVDVADLSDPLALTNIFTGAKGAYLLVPPQMHAEDFIAAADSISSSIASAVRDSGVSH
                     VVALSSIAAQHAERTGPIVTLHRLEEKLKQVPQLSALFLRPAYFMENFMMMIPLVQAM
                     GFFAGGIRGDLKMPMIATRDIGDYAADRLAALDFTGFSTREMLGQRDVSHDEAASAIG
                     AAIGKPKLSYQKFPAFLVEQGIKQLGIPKKTASLMTEMHEAGNEGLLNPQEPRSEQNT
                     TPTSIEAFAQEEFAPAYQAKAASA"
     gene            complement(998..2689)
                     /gene="hutU"
                     /locus_tag="VFR42_12550"
     CDS             complement(998..2689)
                     /gene="hutU"
                     /locus_tag="VFR42_12550"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU0370039.1"
                     /translation="MSASVTNAESATQFSRGPFRAPRGTQLSCKGWQQEAALRMLMNN
                     LDPEVAEKPEDLVVYGGTGKAARNWESFHAIVKALRGLSNDETLLVQSGKPVGIFRTH
                     DYAPRVLLCNANLVGHWSNWDKFNELDRAGLMMYGQMTAGSWIYIGTQGILQGTYETF
                     ASAAAKHFGGDLKGKLVVTGGIGGMGGAQPLAATMNGAAFLGIDVDPERIKKRLKTGY
                     IDVMVNSMDEALRILKNAVRKGQATSVGLVGNCADLIPELAKRGVVPDLLTDQTSAHD
                     PIGGYVPNGMTLEAALALRKKDRDEYKKRSLEAIAEHVQGMLDLQKLGAVTFDYGNNI
                     RTFAYQQGVKDAYNFPGFVPAYIRPLFCEGKGPFRWAALSGESSDIARTDQLVLDLFP
                     KNEHLRRWISLAQKRVKFQGLPSRICWLGYGERDKFGLALNDLVAKGELKAPVVMGRD
                     HLDCGSVASPYRETESMQDGSDAVADWPLLNALVNTASGASWVSIHNGGGVGIGYSQH
                     SGQVTVADGTKEMAVRIERVLTNDPGMGVIRHVDAGYPEAIGFAKQKGVKVPMGL"
     gene            2832..>3712
                     /locus_tag="VFR42_12555"
     CDS             2832..>3712
                     /locus_tag="VFR42_12555"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0370040.1"
                     /translation="MTLHVGIIGGGNISQTHARAACQIPGVQIAAVCGTNTEKVARLA
                     AEHGATPFSDFEKFLAHRPMDFVAIGSPSGLHAEQGIAAARRGLHVLSEKPLDISTAR
                     ADALIAAASESGVKLGVFFQDRFKSDLLRVKQWINDGVLGKPILADARVKWFRPTSYY
                     ADSHWRGTLLLDGGGALINQAVHTVDLLLWLFGDVVGVQGARKTTLHPIEAEDTLVAT
                     LEFGNGAIGTLQATTSVYPGYPRRVELTGSQGTVIIEHDRLLTADLRNPPPDFCSTSD
                     DQNASASSPTVSDVRGH"
BASE COUNT          716 a         1191 c          992 g          813 t
ORIGIN      
        1 tatgcttgaa gtttggggcg aacttcaggc cgatgccgct ttcgcctggt acgccggagc
       61 aaattcttcc tgggcgaacg cttcgatcga agtcggcgtg gtgttttgtt cggagcgtgg
      121 ctcctgggga tttagcaagc cctcgttgcc ggcctcgtgc atttcggtca tcaggctggc
      181 ggtctttttg ggaataccaa gttgtttgat gccttgttcg acgaggaagg ccggaaattt
      241 ctgataggag agttttggct tcccaatggc cgcgccaatc gcgctggccg cttcgtcgtg
      301 cgaaacgtct cgctgtccca gcatttcgcg cgtcgagaag ccggtaaagt ccagcgccgc
      361 cagccggtcc gctgcgtagt caccgatatc ccgcgtggcg atcatcggca ttttcaggtc
      421 gccgcgaatt ccgccggcga aaaagcccat ggcctgcacc agcggaatca tcatcatgaa
      481 gttttccatg aagtaggccg gccgcagaaa aagcgccgaa agctgcggca cttgcttgag
      541 tttttcttcc aaccgatgaa gcgtgacgat cgggcctgtg cgttctgcat gttgcgcggc
      601 gatgctgctc agcgcgacca cgtgcgacac ccccgagtct cgaaccgcac tcgcgatcga
      661 tgagctaatg gaatctgcgg ccgcgatgaa gtcttcggcg tgcatttgcg gaggaaccaa
      721 cagatacgct cccttcgccc cggtgaaaat attggtcagc gccagcggat cgctcaagtc
      781 ggcaacatcc acatcggcgc cgagccgcgc cagcgcgctt aatttcgttg catcccgccc
      841 aacggcgcgt actttttcgc ccttcgccag cagctttcgt gcaactaccg agccggtatt
      901 gccagaagct cccaagacta cgtacattcg tcgctcctat tgatttgatg caagctgttc
      961 ttcccgaagc agctaaagac cgcccgcgac aaaactctta caagcccatg gggaccttga
     1021 cgcccttttg ttttgcaaag ccaatcgctt ccggataacc ggcgtccaca tggcgaataa
     1081 cgcccatgcc cgggtcgttc gtcagaacgc gctcgatgcg taccgccatt tccttggttc
     1141 cgtccgcgac ggttacttgg ccggagtgct gcgaatagcc aatccccacc cctcctccgt
     1201 tgtgaatcga cacccaggaa gctccgctag ccgtgttgac caacgcattg agcaacggcc
     1261 agtcggccac cgcatcggag ccgtcctgca tggattccgt ttcgcgataa ggcgaagcca
     1321 ccgaaccgca atccagatga tcgcgcccca tcacgaccgg agctttcaat tcaccttttg
     1381 ccaccaaatc gttcaacgcg agcccgaatt tgtcgcgttc tccatatccc aaccaacaaa
     1441 tacgcgatgg cagcccctga aatttcacgc gcttttgtgc cagggagatc cagcggcgca
     1501 gatgctcatt cttcgggaaa agatcgagta ccaattgatc ggtgcgtgca atgtccgagg
     1561 actcgccgga aagcgctgcc caacgaaatg gccctttgcc ttcgcagaat agtggccgga
     1621 tgtacgccgg cacaaatccg gggaagttgt aggcgtcctt cacaccctgc tgatacgcga
     1681 acgtccgaat gttgttgccg tagtcaaacg tcaccgcacc cagcttttgc aaatcgagca
     1741 ttccctgaac gtgttcggct atcgcttcta gcgagcgctt tttatactca tcgcgatctt
     1801 ttttgcgaag tgcaagcgcc gcttccagcg tcattccgtt tggcacgtag ccgccgatag
     1861 ggtcgtgcgc gctggtttga tcggtcagca ggtccggcac cacgccgcgc ttcgccagct
     1921 ccggaatcaa gtccgcacag ttcccaacca gcccaaccga agtcgcttgg cctttgcgca
     1981 ccgcgttttt caagatacgc agcgcctcgt ccatgctgtt caccatcaca tcgatgtagc
     2041 cggtcttcag ccgtttctta atgcgttccg ggtccacatc aatgcctaaa aacgccgcgc
     2101 cgttcatggt ggcagctaaa ggctgggctc cgcccattcc gccgataccg ccggtgacga
     2161 ccagcttgcc tttcaggtcg cctccaaaat gtttggccgc agcggatgcg aacgtttcgt
     2221 aagtgccctg cagaattcct tgcgtcccga tgtaaatcca gctgcccgcg gtcatctgcc
     2281 cgtacatcat cagtcccgcg cggtccagtt cgttgaattt atcccagttg ctccagtgac
     2341 cgaccaggtt tgcattgcat agcaacaccc gcggggcgta gtcatgggta cggaaaattc
     2401 ccactggctt gccggactga acgagcagtg tttcatcatt cgaaagcccg cgtagcgcct
     2461 tcacgatggc gtgaaagctc tcccaattgc gtgccgcttt gcctgttcct ccataaacca
     2521 ccagatcttc cggcttttcc gccacctcgg gatccagatt gttcatcagc attctcagcg
     2581 cggcttcctg ctgccagcct ttacaggaaa gctgtgtccc acgcggcgcg cggaatggcc
     2641 cacgcgaaaa ctgtgtcgcg gactccgcgt tcgtcacact cgcactcatc gtattctctc
     2701 catcggccat cttagcgcac cgctggcaaa tctgctgtac gcgcgggaac acccggattt
     2761 gacgtcaaat tgacgttccg gcttggtcgg aataccatgc ggccagcgca ggttaggcga
     2821 gcgcggtttt tatgaccctt cacgttggca tcatcggcgg tggcaacatc tcgcagacgc
     2881 acgctcgcgc cgcgtgccaa atccccggcg ttcagatcgc ggccgtttgc ggtaccaaca
     2941 ccgaaaaagt tgcccggttg gccgccgaac acggcgccac gcccttttcc gacttcgaga
     3001 aattcctcgc gcaccgcccg atggatttcg tagccatagg cagcccctcc ggcttgcacg
     3061 ccgagcaggg aattgccgct gctcgccgcg gcctgcacgt actctctgaa aagccgctcg
     3121 acatctccac cgcgcgtgcc gatgcattga tcgcggccgc gtcggaatca ggggtgaagc
     3181 tgggcgtctt ttttcaggac cgtttcaaat ccgacctgct ccgtgtcaag cagtggatca
     3241 acgacggcgt gctgggtaaa ccgatcttgg ccgacgctcg cgtgaagtgg tttcgaccga
     3301 ccagttatta cgccgactcg cattggcgcg gcacgctgct gctcgacggc ggaggtgccc
     3361 tgatcaatca ggccgttcac accgttgacc tgctgctttg gcttttcggc gacgtggtcg
     3421 gagttcaggg tgcgcgcaaa accactctgc acccgattga agccgaagat accctcgtgg
     3481 caacgctcga attcggcaac ggcgctattg ggaccctgca ggcaaccacc tccgtctatc
     3541 cgggctatcc gcgccgggtc gaacttaccg gctcgcaagg caccgtcatc atcgagcacg
     3601 atcgcctgct caccgccgac cttcgcaatc ctccgccaga cttttgcagc accagcgacg
     3661 accagaacgc cagcgcttct tcgcccaccg tcagcgatgt gcgtggccat ca
//
LOCUS       DASTPM010000708         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1092279, whole genome shotgun sequence.
ACCESSION   DASTPM010000708 DASTPM010000000
VERSION     DASTPM010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092279"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFR42_12560"
     CDS             complement(<1..311)
                     /locus_tag="VFR42_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU0370041.1"
                     /translation="MPEHSLIYRDIAMVFAGAFICGLLAWRLRQPILLGYVLAGLILS
                     PLTPGLKVHVVHTFEVMAEIGVILLMFSVGIHFSIPDLLKVKWVALLGAPLGILLSVG
                     LG"
     gene            complement(360..1247)
                     /locus_tag="VFR42_12565"
     CDS             complement(360..1247)
                     /locus_tag="VFR42_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0370042.1"
                     /translation="MELHQLRYFCAVAEAGSFSRAAELSHVAQPSLSQQILKLEAELG
                     ARLFDRLGRSVRLTEAGKTFLPRARAVLRELEAARGDVSEGKDSISGHVTVGVIPTVS
                     PYFLPAILAAFSKHFPDAAVSVVEEITPVLLDRLRAGAIDLAILALPVRGHEFESLPL
                     LTEPLFAALPKKHKLSRRSQVALHELRKEPFLLLRDGHCFRENAIAACDRARVSPQVV
                     FESGQFSSLLGLVGAGLGISLVPAMAISRTPEVKFVRIADPEATRTIGALTLRGRSLS
                     RAQQAFLSTLRKASSLTDD"
     gene            1399..2889
                     /locus_tag="VFR42_12570"
     CDS             1399..2889
                     /locus_tag="VFR42_12570"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEU0370043.1"
                     /translation="MAEEKKTMTTDAGRPVGDNQNSLTVGPRGPVVFEDFLLFEKMAH
                     FNRERIPERVVHAKGSGAHGYFVCTSKEITKYTTAKLFNAVGKKTPTFIRFSTVGGEK
                     GSADTERDPRGFALKFYTEEGNWDMTGNNTPVFFIRDPLKFGDFIHTQKRDPETNLKS
                     PTMMWDFWSLSPESLHQVTILFSDRGTPHGYRHMDGFSSHTFSLINDKNELFYVKWHF
                     KTKQGIKNFNAQSAEEMRGKDPDYAQRDLFDAIAKGNFPKWRVAVQIMPEKNAETYHI
                     NPFDLTKVWPHGDYPLHEVGELVLDRNPVNYFAEVEQAAFEPRNIVPGMGYSPDKMLQ
                     ARLISYPDAHRYRLGVNYDALPVNKPQCPYHTYHRDGMTRFDENGGKSVNYEPNSFGG
                     PTQELQYTERPKPVTGTVARHDHRSDSDYYTQPGNLFRLMAADAKERLISNIVGSLGS
                     GVPRRIQELQIQHFYKADPAYGTGVAKGLGIDINSVVNKKATVAAD"
     gene            complement(2870..>3093)
                     /locus_tag="VFR42_12575"
     CDS             complement(2870..>3093)
                     /locus_tag="VFR42_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370044.1"
                     /translation="HQIFGTGNGTGRTQKRQFRHSLLSRYADETLPYSSVKAKWTPVQ
                     FADRRGEQKARPIGWVKASLPLPVSQRPQ"
BASE COUNT          764 a          802 c          955 g          572 t
ORIGIN      
        1 cccagcccga cggacaggag aatgcctagt ggtgcgccaa gaagggcaac ccatttcact
       61 ttgagcaaat cggggataga gaaatggatg ccgacggaga acatcaagag gatcacgccg
      121 atttcggcca tgacctcgaa ggtgtgaacg acgtggacct taagaccggg ggtgaggggg
      181 ctgaggatca gtccggcgag gacatagcca aggaggatgg gctggcggag acgccaagcc
      241 aggagtccgc agataaatgc tccagcgaaa accatggcga tgtcacgata gatgagggag
      301 tgttccggca tgtggacgac cgagtgctaa tttgaaaagc gcagaaagtg tagcactagc
      361 taatcgtccg taaggctgga tgctttgcgg agggttgaaa ggaaagcctg ttgggcgcgg
      421 gaaagagagc gaccacgcag agtgagtgcc ccgatggtgc gggttgcttc ggggtcggcg
      481 atgcgcacga acttcacctc tggggttcgg ctgatggcca ttgccggcac cagcgagatg
      541 cccaagccgg cgccgacgag gccgagcaag ctggaaaact gaccgctttc aaaaacgact
      601 tgcggagaga cgcgagctcg gtcgcaggcg gcgatggcat tttcgcggaa gcagtggcca
      661 tcgcggagaa gcagaaaggg ctctttacga agttcgtgca aagcaacctg actgcggcgg
      721 cttagtttgt gttttttggg cagcgcggcg aaaagcggtt cggtgagcag gggcaaggat
      781 tcaaattcat gcccgcgtac cggcagcgcc aagatggcca gatcaatcgc gccggcccga
      841 aggcgatcga gaaggactgg agtaatttct tcgacgacgg aaacagccgc gtcaggaaag
      901 tgtttcgaga aagccgccag aatcgccggc aggaaatacg gagacactgt ggggatcaca
      961 ccgacggtaa cgtggccgga gatggaatcc ttgccctcag agacgtcacc gcgagcggct
     1021 tccaattcgc gcaacacggc gcgtgcgcgc gggagaaatg tcttgcccgc ttcggtgagg
     1081 cgcaccgaac ggccgaggcg gtcgaacaaa cgcgcgccta gctccgcctc cagcttaagg
     1141 atttgctggg aaagcgaggg ctgagcaacg tgcgaaagct cagcggcgcg agagaaactg
     1201 ccggcttcgg cgaccgcgca aaaataacga agctgatgta gttccattta gctttcaagc
     1261 tacggttgaa aacactttcc ttgccatagg ctatgcctat ccattctatc cgaactagat
     1321 attggaacaa tagcagcggg acaattaacc ttcttaaata agcggccgcg gatgcgccgc
     1381 gagatacttg gaggaaagat ggcagaagag aagaaaacta tgaccaccga cgcggggcgg
     1441 cccgtcggag acaatcaaaa ttcattaacg gtagggcctc gaggcccggt tgtttttgaa
     1501 gattttctgt tgttcgaaaa gatggcccac ttcaaccggg agcgcattcc ggaacgcgtt
     1561 gtgcacgcaa agggatcggg cgcgcacgga tattttgtct gcaccagcaa ggaaattacg
     1621 aagtatacga ccgcgaaact gttcaacgcg gtcggaaaaa aaacaccgac gttcattcgc
     1681 ttttctaccg ttggcggaga gaagggttcg gcggacacgg agcgcgatcc gcgcgggttt
     1741 gcgctgaagt tctacacaga agaaggaaac tgggacatga ccggcaacaa cacgccagtg
     1801 ttcttcattc gcgatccgct gaagttcggt gattttattc acacgcaaaa gcgcgatcca
     1861 gagacgaacc tgaagtcgcc aacgatgatg tgggattttt ggtcactttc gccggaatcg
     1921 ctgcaccaag tgacgattct cttctccgat cgcggtacgc cccacggcta tcgtcacatg
     1981 gacggcttca gcagccacac tttcagcttg atcaacgaca agaacgaact tttctatgtg
     2041 aagtggcact tcaaaaccaa gcaaggcatc aagaacttca atgcgcagtc agccgaagag
     2101 atgcggggga aagatcccga ttacgcgcag cgcgatcttt ttgatgcgat tgccaaaggc
     2161 aacttcccga agtggcgcgt cgcggtgcaa atcatgccgg agaagaacgc cgagacgtat
     2221 cacatcaacc cgttcgattt gacgaaggtg tggccgcatg gcgactaccc gctgcacgaa
     2281 gtcggggagc tggtgctgga ccgcaatccg gtgaactatt tcgcggaagt cgaacaggcg
     2341 gcgtttgaac cgcgcaacat cgtgccaggc atgggctatt cgcccgacaa aatgctgcag
     2401 gcgcggttga tttcataccc cgacgcccat cgttatcgtt tgggcgtgaa ctatgacgcg
     2461 ttgcccgtga acaaaccgca gtgcccgtac cacacgtatc accgcgacgg catgacgcgt
     2521 ttcgacgaaa acggcggcaa gagcgtgaac tacgagccga acagcttcgg cgggccgacg
     2581 caggaactgc agtacacgga gcgcccaaaa ccagtgacgg gaactgtggc gcgccacgat
     2641 catcggtcgg acagcgacta ctacacgcag ccgggcaatc tgttccgttt aatggcggcg
     2701 gacgcgaagg aacggctgat cagcaacata gtgggcagct tgggcagtgg cgtgccgcgg
     2761 cgcatccagg agctgcagat ccagcatttc tacaaggccg atccggccta cggaacgggc
     2821 gtggcgaagg gcttgggcat cgacatcaac tccgtggtca ataaaaaagc tactgtggcc
     2881 gctgactgac cggcagcggc aaagaggctt ttacccaccc tatggggcga gctttttgct
     2941 cgcctcttct gtctgcaaac tgaaccggcg tccattttgc tttaacggag gagtacggta
     3001 acgtttcgtc cgcgtatcgc gagagaaggc tatggcgaaa ttgacgtttt tgggtgcggc
     3061 cggtaccgtt accggttcca aatatctggt gga
//
LOCUS       DASTPM010000709         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_400519, whole genome shotgun sequence.
ACCESSION   DASTPM010000709 DASTPM010000000
VERSION     DASTPM010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400519"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2307)
                     /locus_tag="VFR42_12580"
     CDS             complement(<1..2307)
                     /locus_tag="VFR42_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370045.1"
                     /translation="MVADHRAPGLALLLAAFALAGAITGWILVQLLREHVDPASLARF
                     TSTILPNETVVLAEVQASEASRVLATLRDVEAETPVTFGFYPPPPFSVEPTARPPGHE
                     LPSSQRLVENAARLASAIAVRRGTKPRGPSFLRRLREIEDALEWANVSLTMSAEAHHA
                     FTLSAEWLLDNAYLIRDQVTDLRKSLPQKYYGKLPLIAGGPGTGLPRVYYVAAEMVAE
                     TDGALEPEIIRRFLAAFQAITPLDIGELWALPLMLRLQLLECLRALAIQVEQQQTQSE
                     EADFWANRLITAVRHSSPRLLKIMEELMERYPEPTPHFASELVAHLYDDEGALPLVSG
                     WLERSLRSPLLEVMQQEHRYQAVQQTALTNAINSCRRLAQIQWRELFQSTSWAESELA
                     ADPAGVYARLDFETRDRCRGVVEEVARWSNCSEQRTIDLALALAKAAEDEVARHVGYY
                     LIDAGRPALERATSARVPLAERSRRWLRAHAAGAYFGSLFLLTSALVAAPLLFVAGLV
                     PWLTLGLLGFLLLLPASELAVLAVNYFVTLLLPPQVLPKMSFEKEGIPDDCRTLVVVP
                     MLLTTPAAIQNELSRLEIRYLGNTDANLRFSLLTDFADAPRQSMPEDAEYIDIVARGI
                     EELNRRHGAGRFFLFHRGRSWSESEQRWIGWERKRGKLEQLNRFLIGESAPELEGFLC
                     AGDRTQLVGIRFVITLDADTQLLRDTARRLVETLAHPLNQARLSPDGRRVIRGYTIIQ
                     PTVSASLPSARATWFSRIFADPRGIDPYT"
BASE COUNT          526 a          781 c          749 g          465 t
ORIGIN      
        1 cgtgtaagga tcgatcccac gcggatcagc aaagatgcgt gaaaaccatg tcgctctcgc
       61 gctcggaagc gaggcgctga cggttggttg aatgatcgtg tagccgcgaa ttacgcggcg
      121 gccatccggc gaaagccgcg cctgatttag cggatgcgca agagtttcga ccaatctccg
      181 ggcggtatcg cgcagtagtt gggtatcggc atccagtgtg atgacaaacc gaatgcccac
      241 aagctgcgtg cgatcgcccg cacagagaaa tccttccagc tccggcgctg attcgccgat
      301 gagaaatcga ttgagttgct cgagcttgcc gcgcttgcgc tcccagccga tccaacgttg
      361 ttcgctctca ctccatgatc ggccgcgatg gaaaaggaag aagcgccccg ctccgtgtcg
      421 gcgattcaat tcctcgatgc cgcgggccac gatgtcgata tattctgcgt cctccggcat
      481 gctctgccgc ggtgcgtcag caaaatctgt caggagcgaa aagcgcagat tcgcatccgt
      541 gtttccgagg taacgaattt caagccggct gagttcgttt tggatcgcgg ccggggtcgt
      601 cagcagcatt ggcactacca ccagggtgcg gcaatcatcc ggaatgcctt ctttttcgaa
      661 ggacattttg ggcaagacct gcggcggcaa gagcaaggtc acgaaataat tcacggccag
      721 taccgccagc tcactcgcgg gcaagagtag cagaaatcca agtagcccca gtgtcagcca
      781 tgggaccagc ccggcaacga acaggagagg agccgccacc aaagcggacg tgagcaggaa
      841 aaggcttccg aagtaagcgc ccgcagcgtg ggcgcgaagc catcggcgcg accgttcggc
      901 caagggcact ctggcgctgg tagctcgctc gagcgccggc cggcccgcgt cgatcaaata
      961 atagccgacg tggcgcgcaa cttcgtcctc cgcggccttc gctagcgcga gggcgagatc
     1021 gatggtcctt tgttccgaac aatttgacca tcgggcgact tcctccacca caccccggca
     1081 tcgatcacgc gtctcaaagt ccaggcgggc gtaaacgccg gccggatctg cagccaactc
     1141 gctttccgcc cagctggtcg attggaaaag ttcccgccac tggatttgcg cgaggcggcg
     1201 acaactattg atcgcattag tgagcgcggt ttgctgcacg gcctggtaac gatgctcctg
     1261 ctgcattacc tcgagcagcg gcgaacggag cgagcgttcc agccagccgc tcaccaacgg
     1321 gagcgcccct tcgtcatcgt agagatgcgc caccagttcg ctcgcaaaat gcggcgtcgg
     1381 ctcgggatac cgttccatca gttcttccat tattttgagc aatcggggcg agctgtgacg
     1441 gacggcggtg atgagccgat ttgcccaaaa gtcggcctcc tcgctttgcg tttgctgttg
     1501 ctcgacttga atcgcgagcg cgcgcaggca ttcgagtaac tgcaatcgca acatcagcgg
     1561 cagagcccag agttctccga tgtccagtgg cgtgatcgcc tgaaacgcgg cgagaaatct
     1621 ccggatgatc tcgggctcca aggctccgtc ggtctcggcg accatttccg cggccacata
     1681 ataaacccgt ggcaaccccg tacctgggcc gccggcgatc agaggcagct ttccatagta
     1741 tttttgcggg agactcttcc gcagatcggt cacctggtcc cgaatcaaat aggcgttatc
     1801 gagcagccac tccgccgaaa gagtgaaggc gtggtgcgcc tcagccgaca ttgtgagact
     1861 cacgttcgcc cattccagcg catcctcgat ttcgcgtaac cggcgcagaa aagatggtcc
     1921 gcgcggcttc gttccgcgcc tgactgcgat tgcgcttgcg aggcgcgccg cattttccac
     1981 gagacgctga ctggaaggaa gttcatgacc gggcggtcgt gcggtcggtt cgacagaaaa
     2041 aggtggcggc ggataaaaac cgaacgtcac gggcgtctcg gcttccacgt cccgcaaagt
     2101 cgccagcacg cgcgaggctt cgctcgcttg gacttcagct aggacgaccg tttcattcgg
     2161 cagaatcgtg ctcgtgaatc tggccaggct cgccgggtca acatgttccc gaagcaattg
     2221 gacgagaatc cagccagtga tcgcacctgc taacgcgaac gcagcgagta gcagggccaa
     2281 cccgggcgcc cggtgatccg ccaccattcc gcgctcccac aagataaacg cgccgacgac
     2341 caggctgaga accgaagcgg cggccgatcc gccgatcgcc gaaatcccat gttcctcgac
     2401 gcgctgccgg cctttggccg aagcgtggat cgccgcggcc cgacgaaacc gggcgcgatg
     2461 gagattccgc agtacggccc gaccaactcc cacactttgg aaaatgccga cgaccaaaac
     2521 g
//
LOCUS       DASTPM010000710         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_948241, whole genome shotgun sequence.
ACCESSION   DASTPM010000710 DASTPM010000000
VERSION     DASTPM010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948241"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VFR42_12585"
     CDS             complement(<1..643)
                     /locus_tag="VFR42_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0370046.1"
                     /translation="MKKSATARALFALCRVVVVGDTCISEEGIAAIVWRDKRYHVCGS
                     AHGFYDAGELIRKHQPDVLLIEPFLEDHDGIRWIKDLATEYPRIRILIVTGQSESLYA
                     ERALHAGAAGYWMKNGSADELLRAVETVAGGGIYVSPLIASLALERFVHRKALPHGVN
                     LLSDRELAVFALIAAEQGVGRIAKKLGISRTTVETHCEHIKMKLGYHNAQALKD"
     gene            complement(789..2060)
                     /locus_tag="VFR42_12590"
     CDS             complement(789..2060)
                     /locus_tag="VFR42_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370047.1"
                     /translation="MKQVIHLKSTINAWVKGPIRYSPLARVFLLAAVLFACLALSQTA
                     PAANPGPGTPATSGSGGAIDGALNFTNTALLRRVPTIITFDAPGSGTGPGQGTITFAI
                     NPAGTISGRYADASGAIHAIIRSPDGAIITFDAPGAGTGPRQGTRPFSINPAGATAGY
                     YNDASGVFHGFLRTPDGVITTFNVPGAGTGPAQGTFAGNIIPGDVIAGRYVDASDVAH
                     GFLRAPDGTITTFDAPNAGTGPGQGTFVFTGFCLNPAGAIAGTSLDASDVYHGILRAP
                     DGTITTFDVPGAGTGPFQGTLPLGINQAGTIEGDYIDSGDVNHGFVRASDGTITTFDV
                     PGAGTGPFQGTSPQGINAQGAVTGEYADASDVNHGFVRALDGTLTTFDVPAAGTGPFQ
                     GTIPLSNNPVGAITGYYIDASDVTHGFLRTP"
     gene            2353..2949
                     /locus_tag="VFR42_12595"
     CDS             2353..2949
                     /locus_tag="VFR42_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370048.1"
                     /translation="MRSRGYTVHDAQDLTQSFFAYLIEHKIYARVDRQKGRFRSFLLA
                     SMKNFLAHASDRERTLKRGGGQNFLPLHEEQIENAESLFQTHSSSSEDRVFDRSWAAA
                     LVTAGLERLAADYKGESKEKLFRELRAFLGGGAGPPRPYAELAVRLGMSESTLRTHVT
                     RLRARYREALHAEVRRTVATEAQAQEELHVLLRVLTEN"
     gene            3016..>3440
                     /locus_tag="VFR42_12600"
     CDS             3016..>3440
                     /locus_tag="VFR42_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370049.1"
                     /translation="MDAKGDCVACFLRTALDESVVETKQLPSLVFGDFEVEQRPDGSY
                     WELGHGAMGTTYLALDKVLRRMVALKVIEVPAVPTSRDAQAVRERFLREARAAAALRH
                     PNVAAVYQFGAAPDGNRCFYAMELVEGETLETRVRRHGPL"
BASE COUNT          711 a          939 c         1041 g          749 t
ORIGIN      
        1 catccttgag cgcctgggcg ttgtgataac cgagcttcat tttgatatgc tcacaatgcg
       61 tttccacagt ggttcggctg atgccgagct ttttcgcaat gcggcccaca ccttgttcgg
      121 cagcaattaa agcgaatacc gccagttcgc ggtcgctcag gagattcacg ccgtgtggaa
      181 gggccttgcg gtggacaaac ctctcgagtg ctagggacgc gatgagtggg ctaacgtaaa
      241 ttcccccgcc ggccacagtt tcaaccgcgc gcaataattc gtctgctgat ccgtttttca
      301 tccagtagcc ggccgcgccg gcatgcagag cgcgctccgc atagagcgat tcagattgac
      361 ccgtaacaat gaggatacga atgcgcggat attcggtggc gaggtctttg atccagcgga
      421 tgccgtcatg atcttcaagg aacggctcga tcagcaaaac gtcgggctga tgttttcgga
      481 tcagttcacc cgcatcatag aaaccgtgtg cgctgccgca aacgtggtac ctcttatcgc
      541 gccagacgat tgcagctatc ccctcttcgc tgatgcacgt gtcccccacc accacgaccc
      601 ggcacagcgc aaatagtgcg cgtgctgtcg cgcttttttt cacggatatg gagcttttaa
      661 caaattggtc ccgtgtaatg caatattaaa tttgcgtttt tagcgaaaaa attcataggc
      721 cttcgccttc acatcgagca tgcggtgaaa gcagtgctta agccaccacg gtatcggcgg
      781 atgagcggct acggggtccg caggaaaccg tgagtcacat cgctcgcgtc aatatagtat
      841 ccggtgatcg ccccgaccgg attgttggaa agagggatag tgccttggaa gggacctgtg
      901 cccgcggcag gaacatcgaa cgtagtgagg gtaccatcca acgcgcgcac gaacccgtgg
      961 ttgacgtcac tcgcgtccgc gtactctcct gtgaccgccc cttgcgcgtt gatgccttgg
     1021 ggtgaggtcc cttggaaagg gcctgtgccc gcgcccggaa cgtcgaacgt ggtgatcgtg
     1081 ccgtccgagg cacgcacgaa cccatgattt acatcacccg aatcaatgta gtctccctcg
     1141 atcgttccgg cttggttgat ccccagcggg agtgtgcctt ggaaagggcc tgtgcccgcg
     1201 cccggaacgt cgaacgtggt gatggtgccg tccggggcgc gtaggatgcc gtgatataca
     1261 tcgctcgcgt caagcgaagt tcccgcgatc gcccctgctg gattgaggca aaagccggta
     1321 aaaacaaagg tgccctggcc ggggcctgtg ccggcgtttg gagcatcaaa cgtagtgatg
     1381 gtgccgtccg gagcgcgcag gaagccgtga gctacatcgc tggcgtcaac gtagcgtccg
     1441 gcgatcacgt cccccggaat gatgttgcca gcgaaggtac cctgggcggg acctgtgccc
     1501 gcacccggga cgttgaaggt agtgatcaca ccgtccggag tacgcaggaa accgtgaaac
     1561 acaccgctcg cgtcgttata gtagccagcg gtagcccctg ccgggttgat gctgaagggt
     1621 cgagtgccct ggcggggacc tgtccccgcg cccggagcgt cgaaagtaat gattgcgccg
     1681 tccggactgc ggatgatagc gtgaatcgca ccgcttgcgt cggcgtagcg tcccgagatc
     1741 gtccctgctg ggttgatggc gaacgtgatt gtgccctggc ccgggcctgt accagagccc
     1801 ggagcatcga atgtgatgat ggtgggcaca cgcctaagta aggccgtgtt ggtgaaattc
     1861 agcgcgccat cgatcgcgcc accggacccg ctggtcgcgg gggtgcccgg gccgggattg
     1921 gccgcgggcg ctgtttgcga cagcgcaaga caggcgaaga gaacggcagc cagaaggaaa
     1981 acgcgtgcta acggcgaata cctgatcggg ccttttaccc aagcgtttat tgtcgactta
     2041 agatgaatga cctgtttcat atgctttgtt tctgttgatg gtaatagttg ttttcccttt
     2101 acaaacggat tctggagcgt gcgctacacg gaatctgaat ttttctcgcg acttgctttt
     2161 cttcttgcgg tcttgataga aactggtgac gtgaagacgc tggcagggaa cgagtgtcgc
     2221 cagagtgatt tttttccttc gactcaatgg agcgtagttc tcgcgtcggg acggagtgga
     2281 gcagaaccag aagttgcgca agccgcgcta gcggagcttt gccaaatata ttgggcgccg
     2341 ctttacggtt tcgtgcgcag ccgcggttac accgtgcatg atgcgcagga tctgacgcaa
     2401 agctttttcg cgtacctgat cgaacacaaa atatacgctc gcgtcgatcg gcagaagggc
     2461 aggttccgtt cgtttttgct tgcttccatg aaaaattttc ttgcgcacgc ttccgaccgc
     2521 gaacggacgc tcaaacgcgg cggcggacaa aattttcttc ctctgcatga agagcagatc
     2581 gaaaatgcgg agtcgctttt ccagactcat agttctagta gtgaagatcg agtcttcgat
     2641 cgtagctggg cagctgcgtt ggtcaccgct ggactggaac ggctcgcggc agattacaaa
     2701 ggtgaatcca aggagaagct cttcagggaa ttgagggcat ttcttggcgg tggcgctggg
     2761 ccgccacggc cttacgctga gctggcggtt cgtctgggaa tgagtgaatc aacacttcgc
     2821 acccacgtca ctcgcctccg agcgcgttat cgggaagcgt tgcacgctga ggtgcgccga
     2881 acggtggcaa ctgaagcgca agcgcaggaa gagctgcacg tgttactccg tgtgttaacg
     2941 gaaaattaag aaatacgtgc catggcaaca ccaccagcat cagcaaacac ggtttgctgg
     3001 gcatgcggcg agccgttgga cgcaaagggc gactgcgtag cttgtttcct ccggaccgcc
     3061 ttagacgaat cagtcgtcga aaccaagcag cttccctcgt tagtcttcgg cgacttcgag
     3121 gtcgagcaac gcccagacgg ctcttactgg gaactcggtc acggcgccat ggggacgacc
     3181 tatctggccc tggacaaagt gttgcgtcgg atggtcgctc tgaaggtcat cgaggtgccg
     3241 gcggttccga ccagtcgaga cgcacaagcc gtacgcgaac gctttttacg tgaagcccgc
     3301 gcagcggccg cgctgcggca tccaaacgtt gctgctgttt atcagtttgg agctgcacct
     3361 gatgggaacc gttgctttta cgcaatggaa ttggtcgaag gcgaaacgtt agagactcgt
     3421 gtgcgaagac acggtccgct
//
LOCUS       DASTPM010000711         2726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_256072, whole genome shotgun sequence.
ACCESSION   DASTPM010000711 DASTPM010000000
VERSION     DASTPM010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256072"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFR42_12605"
     CDS             complement(<1..215)
                     /locus_tag="VFR42_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370050.1"
                     /translation="MPRVRRILVKSSAGSYEVVCGEGVLTRFAAELARLGKFSSVQVA
                     TSAKVWAAVGKQVLRGLRGLNPRVHKF"
     gene            271..2028
                     /gene="nadB"
                     /locus_tag="VFR42_12610"
     CDS             271..2028
                     /gene="nadB"
                     /locus_tag="VFR42_12610"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEU0370051.1"
                     /translation="MNSQIPRATMIPAGNPMTSVPKTYHADYAVVGSGVAGLRAAIEL
                     SSAGTVLVLAKSDLSESATAYAQGGIAVALSDEDEIGLHEQDTLAAGDGLCRPQSVAL
                     LVEEGPKYITQLIEWGTEFDRAGTKLAFTREAAHSRSRILHAHGDSTGREISRALLAR
                     AHALPHLHLRAHAFTTGLIVENGRVCGLRFLDETDGSFHEVRAGAVLLATGGLGQLYR
                     ETTNPAVATGDGMAIAYEAGAVLSDMEFVQFHPTALAIKGAPRFLLSEALRGEGGILR
                     NIDLERFMKRYAEAQELAPRDVVARAIVSEMHRTSAQHVYLDMTAKSAEFLQKRFPRI
                     YSTCLSYGLDLATDMAPVCPAAHYMMGGVKTDLCGRTSVPGLYAAGETADTGVHGANR
                     LASNSLLEGLVFGARAGDAMLTDAPAVKHKSGPLPGSLAPKPGNSAPGTLPAHPEKPA
                     ASAQLAQIRDLMWRHVGILRSGKELSAAIGLLEKMELHAVATSEKRFSASTGDGHGHT
                     GAPGLGGVVATTEQDHATRADFELRNLHTLATLIARSALAREESRGSHYRSDFPYRDD
                     DLFQKHSLIQRSKDVTFEV"
     gene            complement(2059..>2726)
                     /locus_tag="VFR42_12615"
     CDS             complement(2059..>2726)
                     /locus_tag="VFR42_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370052.1"
                     /translation="LLACGLFKFHLIVPFAAILLFRRKWAALGGFTAIASLLLVLCLE
                     ISGVAILREYPRVLFFDGTHRQMMGFHPDFTPNIHGFLYLIAKNVVSAALLGSLVALF
                     SLLALWWAAKNWRNDRFGFSFAACLLATLLASYHLYNYDLVLVLLPVAIVCGELAQRG
                     HLLSGTPLLTSILLALFVPPVHFVLVSNGIYALMFVPILLLFLVIVRLNRADSPAQRT
                     VHA"
BASE COUNT          603 a          850 c          777 g          496 t
ORIGIN      
        1 ttaaatttgt gaacgcgcgg attcaatccg cgcagtccgc gcaagacctg cttccccacc
       61 gcggcccaca cctttgccga ggtcgccacc tgcacgctgg agaatttccc gagccgcgca
      121 agttccgcgg caaatcgcgt cagcactcct tcgccgcaca ctacctcata gctcccggcg
      181 gaagacttca ccaaaattcg tcgcactcgt ggcattggaa tctttccact ctaacatacc
      241 gcttgcgccg gtctacgcga gcggcgccgc atgaactcgc aaattcctcg tgctaccatg
      301 attcccgctg gcaacccaat gacctccgtc cccaagacct atcacgccga ctacgccgta
      361 gtgggttccg gcgtggccgg cctgcgcgcc gcaattgaac tcagctccgc cggaacggtt
      421 ctcgtcctgg ccaaatccga tctcagtgaa tccgcaaccg cgtatgcgca gggcggcatc
      481 gccgtcgcgc tcagcgacga agacgaaatc ggcttgcacg aacaagacac gctggccgcc
      541 ggggacggtc tctgccggcc gcagtcggtc gcgctgctgg tcgaagaagg tcccaagtac
      601 atcacgcagc tcatcgagtg gggcacggaa ttcgatcgtg caggcaccaa gctggccttc
      661 acccgcgaag ctgcgcacag ccgttcgcgc attttgcacg cgcacggcga ttcgactggc
      721 cgcgaaatca gccgcgccct gctcgctcgc gcccatgcgc tgcctcattt gcatttgcgc
      781 gctcacgcgt tcaccactgg actgattgtc gagaacggcc gcgtctgcgg cttgcgtttt
      841 ctggacgaaa cggatggctc atttcacgaa gtgcgtgcag gagctgttct ccttgccacc
      901 ggcggcctcg gccagctcta ccgtgagacc accaatcctg ccgtagcgac tggcgacggc
      961 atggcgatcg cttatgaagc gggcgccgtt ctctcggaca tggagtttgt gcagtttcat
     1021 ccgaccgcat tggctatcaa gggcgcacca cggtttctgc tttctgaagc gctacgcggc
     1081 gagggcggaa ttctccgcaa catcgacctg gaacgcttca tgaagcgcta cgcggaggcg
     1141 caggagctgg cgccgcgcga tgttgtggct cgggccattg tcagcgagat gcatcgcacc
     1201 agcgcgcagc acgtctattt ggatatgacg gcaaagtccg cggaatttct gcaaaagcgt
     1261 tttccgcgca tttactccac gtgcctgagc tacggtctgg acttggccac cgacatggct
     1321 ccggtatgcc ccgcggcgca ctacatgatg ggcggagtca aaacggattt gtgcggacgc
     1381 acttcggttc ccgggcttta cgccgctgga gaaaccgccg ataccggcgt acacggtgcc
     1441 aaccggttgg ccagcaattc ccttctggaa ggcttggtat ttggcgcgcg ggccggcgac
     1501 gccatgttga cggatgcccc ggcggtcaaa cataaatccg ggccgctgcc cgggtctttg
     1561 gcgccgaagc ccggcaactc cgcgccgggc acgctacccg cgcaccctga aaagccggcg
     1621 gcatccgcgc aacttgcgca aattcgcgat ctgatgtggc gccacgtcgg aattttgcgc
     1681 agtggaaaag aactctcggc agcaatcggg ttgctggaaa agatggaact gcacgcagtg
     1741 gcgacttccg aaaaacggtt ttccgcgtca acgggcgacg gacacggcca cacgggcgcg
     1801 cccgggctcg gcggagtggt tgcaactacg gagcaggatc acgcaactcg cgcggatttt
     1861 gaattgcgca accttcatac gctcgcgacg ctcatcgccc gatcggcgct ggcacgcgaa
     1921 gaatcccgcg gcagccacta ccgctcggac tttccctatc gcgacgacga tctttttcaa
     1981 aaacattccc tgattcagcg ttccaaggac gtgaccttcg aagtctaggc gcgcgtgcgc
     2041 tgagcgacga tgcctctttc aagcgtgaac ggttcgttgc gctggcgagt cggcgcggtt
     2101 gagccttacg atcacgagaa agagcagcaa aatcggcaca aacataagcg catagatgcc
     2161 attgctgacc agcacaaagt gaaccggcgg aacaaacaac gcgagcaaaa tcgacgtgag
     2221 tagcggcgtt cccgagagca agtgtccgcg ttgcgctaac tcgccgcaaa caatggcaac
     2281 aggcagtaaa accagaacca agtcgtagtt gtacaagtga tagctcgcca ggagcgtagc
     2341 cagcaggcac gctgcaaagg aaaagccgaa gcgatcattt cgccagttct ttgcggccca
     2401 ccagagcgca agcaaagaaa acagcgctac cagggacccc agtaacgccg cggacaccac
     2461 atttttcgca atcagataca gaaacccgtg aatgttgggc gtgaagtccg gatggaaacc
     2521 catcatctgg cgatgtgtgc cgtcaaaaaa cagcactctc ggatactcgc gaagaattgc
     2581 aacccccgag atttccaggc aaaggacaag cagcagtgag gcgatagcgg tgaaccctcc
     2641 gagtgcagcc cattttcggc ggaataaaag aatcgccgca aaaggaacaa tcaggtgaaa
     2701 tttgaaaaga ccacaagcta gcaaac
//
LOCUS       DASTPM010000712         3393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1438675, whole genome shotgun sequence.
ACCESSION   DASTPM010000712 DASTPM010000000
VERSION     DASTPM010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438675"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFR42_12620"
     CDS             complement(<1..631)
                     /locus_tag="VFR42_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370053.1"
                     /translation="MKTLLGLGTGVLALGALGGACAVAQSAAGEGTAELREIRSEGQK
                     VLTEAQIAALSGLQTGTQVGRAELQAGADKLVGSGLFATVKYDFKTRNEGVSLTFHVA
                     ESPRVPAYYDNFPWFGDSELNDAIRARLPFFDGMLPEGGNVLDEAAEAIHRLLASHNL
                     QYVVQHAVTANPLGDGNIQQFSVEGTALKIASLNFSDAALATNSGVHEHL"
     gene            complement(862..2757)
                     /gene="glmS"
                     /locus_tag="VFR42_12625"
     CDS             complement(862..2757)
                     /gene="glmS"
                     /locus_tag="VFR42_12625"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU0370054.1"
                     /translation="MCGIVGYIGPKKVVPVVIEGLRKLEYRGYDSAGIAVVNTNGANG
                     GAGKVDIRRASGKLRNLEEAIQKAPIEGSYGIGHTRWATHGRPTEENAHPHRDCSGQV
                     VVVHNGIIENYLELKEQLQREGHKFVTETDTEIVAHLVEKNLKGANGGTIPLEEAVRK
                     SLGQIRGIYALVFLSTTDPNKIVAARQGPPAVVGLGDGEYFVASDIPALLEHTRNMFF
                     LADGDIAVLTAGGVRVMDHDGKTVERKPHHVAWDPIMAEKGGYKHFMQKEIFEQPRAV
                     RDTLLGRISQDTGKIYLGEMEITEQELREFKSVRIVACGTSWHAGLAGKFMIERLARL
                     PVEVDYGSEFRYRDPILDSKTLTVCISQSGETADTIAAQREARQKGSPTLAICNVMGS
                     MITREAAGTILTHAGPEIGVASTKAFTSQLTALMLLAIHLGEVRGALSDDAGKNLLQE
                     FTRMPHKIEQVLQPDSAEFYEQLARQFFRCSDFLYLGRGIHYPIALEGALKLKEISYI
                     HAEGYPAGEMKHGPNALIDENLPVVVLATRDDNDPASVTLYEKSVSNIKEVKARDGIV
                     ISIVTQGDHLAREASDHLIELPPAPELLVPLLEILPLQLLAYHIAVRRGCDVDQPRNL
                     AKSVTVE"
     gene            complement(2920..3162)
                     /gene="purS"
                     /locus_tag="VFR42_12630"
     CDS             complement(2920..3162)
                     /gene="purS"
                     /locus_tag="VFR42_12630"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU0370055.1"
                     /translation="MKAHVWVMPKRTVLDPQGQTIQHALSGLGYAAITDVRQGKFFVL
                     NLEGWSLDEAQKQLEKISRDVLTNPVIEEYRFEIVE"
BASE COUNT          666 a         1093 c          870 g          764 t
ORIGIN      
        1 aaagatgctc gtgcacgccc gaattcgtag ccagcgccgc gtcgctgaag ttcaagctcg
       61 caatctttag cgccgtgccc tcaacggaaa attgctggat gttgccgtct cccagcgggt
      121 tcgccgtcac cgcgtgctgc accacatatt gcagattgtg gctcgccaac aatcgatgaa
      181 tcgcttcggc cgcctcgtcc agcacgttcc cgccctccgg cagcatcccg tcaaaaaaag
      241 gaagtcgcgc gcggatcgca tcattcaact ccgaatcacc aaaccacgga aaattgtcgt
      301 agtacgccgg cacccgcggc gactccgcca cgtgaaacgt cagcgacact ccctcatttc
      361 gcgtcttgaa atcgtatttg accgtcgcaa acagcccgct gcccaccagt ttgtccgctc
      421 cagcctgcag ctccgcacgc cccacttgcg tccccgtctg cagtccgctc agcgcagcaa
      481 tttgcgcctc cgttagtacc ttctgccctt cgcttctaat ctctcgtagt tccgcagtcc
      541 cttcgcctgc cgcggattgc gcaaccgcgc atgctccgcc cagtgcaccc agcgccaaca
      601 cacctgtacc gagacccagt aacgtcttca tgcgcctatt ctaatccgta ttgaaagatg
      661 tggcagggtt ctttaggagg cgaaggcttc agccgtcgcc ttcgcgctcc tcccgatgtc
      721 gtgattccga ccgtagaggg atttccggca acggcaagga gcgcggtctt gtcttcttcg
      781 ggtaaatcct ctaaaaagcc aggaggaagt gatttgagga ctacggtagt gcctgcgggg
      841 gactctgggc ccgtcatctt cttactctac cgtgacggac ttagccaaat tccgcggctg
      901 atccacgtcg cacccgcgtc gcaccgcgat gtggtatgcc agcaactgca gcggcaaaat
      961 ttccagcagc ggcaccagca actccggcgc cgggggcagc tcgatcaaat gatccgacgc
     1021 ttcccgagcc agatgatcgc cttgcgtcac aatcgaaatc acaatgccgt cgcgcgcttt
     1081 cacttcttta atgttcgaca cacttttttc atagagcgtc accgaagccg ggtcgttgtc
     1141 atctcgcgtc gccagcacca ccaccggcaa attttcgtca atcaaggcgt tcggcccatg
     1201 cttcatttcg cccgccggat atccttccgc gtgaatgtac gaaatctcct tcagcttcag
     1261 cgcgccttcc agcgcaatcg gataatgaat cccgcgtccg agatacagaa aatccgagca
     1321 acgaaaaaac tgccgcgcaa gttgctcgta aaattcagcg ctgtctggct gcagcacttg
     1381 ttcgatcttg tgcggcatgc gggtgaattc ctgcagcaga tttttacccg catcgtcgga
     1441 cagcgctccg cgcacttcgc ccagatgaat cgccagtagc atcagcgccg taagctgcga
     1501 cgtaaacgct ttcgtcgaag ccactccaat ctccggtccg gcatgcgtca aaatcgttcc
     1561 cgcggcttcg cgcgtaatca tcgaacccat cacgttgcag atcgccagcg tcggcgagcc
     1621 tttttgtctc gcttcgcgct gcgctgcaat cgtgtccgcg gtttcgccgg actgactgat
     1681 gcaaaccgtc agcgtcttgg aatccagaat cggatcgcgg tagcgaaact cgctgccgta
     1741 gtccacttcc accggaaggc gcgccagccg ctcgatcatg aactttccgg ccagccccgc
     1801 gtgccagctc gttccgcaag ccacaatgcg cacgctcttg aattcgcgca gttcctgttc
     1861 ggtgatttcc atttcaccca gataaatctt tccagtgtcc tgcgaaatgc ggcccagcag
     1921 tgtgtcgcgc actgcgcgcg gctgctcgaa aatctccttc tgcatgaaat gcttgtagcc
     1981 gcccttttcc gccatgatgg ggtcccacgc gacgtggtgc ggcttgcgct ccaccgtttt
     2041 gccgtcgtgg tccatcacgc gcacaccgcc ggccgtcagc acagcgatgt cgccgtccgc
     2101 caggaaaaac atgttgcgcg tatgttccaa cagagcggga atatcgctgg ctacaaaata
     2161 ttcgccgtcg ccaagaccca ccactgcggg cggcccctgc cgtgccgcca caatcttgtt
     2221 cggatcagtg gtggacaaaa acaccagtgc gtaaattccg cgaatctggc caagcgattt
     2281 ccgcaccgct tcttcaagcg gaatcgttcc gccattcgcg cccttcagat ttttctctac
     2341 cagatgcgcg acgatttccg tgtccgtttc cgtaacgaac ttgtggcctt cgcgctgcag
     2401 ctgctctttc agctccaggt aattttcaat gatcccgttg tggaccacga cgacttgccc
     2461 ggagcagtcg cggtgcgggt gcgcattttc ttccgtgggc cggccgtgcg tcgcccaccg
     2521 cgtgtgtccg atcccatacg acccttcaat tggcgctttc tgaatcgctt cttccaggtt
     2581 gcgcaatttt cccgaagcgc ggcgaatgtc caccttgccc gcgccgccat tcgcgccgtt
     2641 ggtgttcacc acggcgattc ccgcggaatc gtagccgcgg tattccagct tgcgcagtcc
     2701 ttcgattacg acggggacaa ccttcttcgg gccgatatag cccacaatgc cgcacatgcg
     2761 gtgtctcctc gaatttctct tctaaagcgt agcacgcgag cgaaacaggc tggatagctc
     2821 aaactactga tgcagcgaat cgtggctggg gtggttgaat gattcctggt aatggcgccc
     2881 taaggccggc agctttctct ggccggggtt tggcgcggct tactccacaa tctcgaaccg
     2941 atattcctca atgaccggat tggtcagcac gtccctggaa atcttctcaa gctgcttctg
     3001 cgcctcgtcg agcgaccacc cctccaagtt cagcacaaag aattttccct gccgcacatc
     3061 ggtaatcgcg gcatagccga gtccggaaag tgcgtgttga atggtttgtc cctgcggatc
     3121 cagtaccgtg cgcttcggca tcacccaaac atgagctttc atgtggcaat tataacggag
     3181 gacagcaaac ccaggccagt cgccccagga gcggggctcg gactagaata ggagcgaggg
     3241 agatgcgcat gagaaaggtc ttccttcttc tgttgtttct gccgttgtcc gccggagccc
     3301 aaagtccctt cgatggaacg tggttgattg acccagcaaa ttttaaaccg ccgcaagagc
     3361 cagcggaatt cctgctggcc gatgggatgt ttc
//
LOCUS       DASTPM010000713         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_689229, whole genome shotgun sequence.
ACCESSION   DASTPM010000713 DASTPM010000000
VERSION     DASTPM010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689229"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFR42_12635"
     CDS             <1..800
                     /locus_tag="VFR42_12635"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU0370056.1"
                     /translation="MIGLIGMFVVHPRDAHSPRVDHDYGIVLQEWAVLPNNSVPNTAN
                     MEFNWLTFNGVAAPLTTPLLARLGSRVRLRFVNLGMDHHPIHLHGNQFVVTGTEGGRA
                     PESTWYPSNTVLVGVAQARVVEFDAKYPGAWMIHCHLPHHMMNSMMDLLRDRQIMTSD
                     QTESAAMSQMLALAARAQFQHQHPSPVAPGAEKVPGFPQDAFMEMGMDELVQKPETHG
                     LPKNWSAGMMGMMTMVRVLPDQEFEEIAALKKSGTTPASAEEHRHGS"
     gene            952..2307
                     /locus_tag="VFR42_12640"
     CDS             952..2307
                     /locus_tag="VFR42_12640"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0370057.1"
                     /translation="MPGMQMQHEPREKTSTLEFPRLGRAQTKSGTTELFTLEHALETA
                     RQNNPTLRQAEAGIRAARARTQQAALYPNPTVGYSGDEIRGGESHGGKQGFFVEQTMV
                     TGGKLSRARDVFAKEAQLAEIEAEEQKIRVETAVRIAFYRVLTTQELADARADLVRIA
                     GETLETERHLQNTGQADETEILAAELETSRMKLSARTMENTLREEWRALASVLGQPDL
                     PLQTVSGDLERGWPELDEHQVETVATQSPANRIASAATERAQAELTRARREKIPDILA
                     RAGLDYNHEPLDNPLLRTGWQWNAQLAVELPIFNRNQGNIASARANIDRAESEKQRVS
                     LTLRERASSVFDEYANARLTAEEYRENILPLAKKSYTMMFERYGEMLASYPRVLENKR
                     KLFELQSEYIASLETVWTTGLSLQGFLLTDGLEAPTQPGEMDRTIRETNLPTPERGRL
                     P"
BASE COUNT          543 a          789 c          665 g          472 t
ORIGIN      
        1 aaatgattgg cctgatcggc atgtttgtcg tgcacccgcg cgacgcgcac tcgcctcgcg
       61 tggaccacga ctacggcatc gtgctgcagg agtgggccgt gctgcccaac aattccgtcc
      121 cgaacacggc caacatggag ttcaactggc tcacatttaa cggcgttgcc gcgccgctta
      181 cgactccgct cctggcgcgg ctcggcagcc gagtacgcct gcgcttcgtg aatctcggca
      241 tggatcacca tccgatccat cttcatggca accagttcgt tgtgacgggt acggaaggcg
      301 gccgcgcgcc cgagtcgacg tggtacccgt cgaacaccgt gctcgtcggc gttgcgcagg
      361 ctcgcgtcgt cgaattcgat gccaagtatc cgggtgcgtg gatgattcac tgccacctgc
      421 cgcaccacat gatgaattcg atgatggact tgctgcgcga ccgccagatc atgacctccg
      481 atcagactga atccgctgcg atgtcgcaaa tgctagcact cgctgcccgc gcgcaattcc
      541 agcaccagca tccgtcgccc gtcgcgcccg gcgctgaaaa agttcccggc tttccgcaag
      601 acgctttcat ggaaatgggc atggatgaac tggtgcagaa accggaaacc cacggcctgc
      661 cgaaaaactg gagcgccggg atgatgggga tgatgaccat ggtgcgtgtg ctgcctgacc
      721 aagaattcga agaaatcgcc gcgctgaaaa aatccggaac gacgcctgcc agcgcagagg
      781 agcaccgcca tgggtcttag tcgaactctt tctatgcaca atccgccaaa catagttcgt
      841 ccgacaggcg ccccatcctc gattttggag ggtgggaact ttcttttctc tcttctattc
      901 gtcgcgattc ttttttcttg cgcgtctctc tcggctcagg agcaaccgtc gatgcccggc
      961 atgcaaatgc aacacgagcc ccgtgagaaa acgtccacac tggaatttcc acgactgggc
     1021 cgcgcgcaaa caaaatcggg tacgacggaa ctcttcactc ttgagcacgc tctcgaaacc
     1081 gcgcggcaaa acaatcccac gctccgtcaa gcggaagcag gcatccgcgc cgcccgcgcg
     1141 cggacgcagc aagcggcgct ctatcccaat cccacggtcg gttactccgg cgacgaaatt
     1201 cgcggcggcg aatcgcatgg cggcaagcaa ggcttcttcg tcgagcaaac catggtgacc
     1261 ggcggcaaac tctcgcgcgc acgtgatgtc ttcgccaaag aagcgcaact tgccgaaatt
     1321 gaagccgaag aacagaaaat tcgcgtggaa acggctgtcc gcatcgcctt ctatcgcgtg
     1381 ttgaccacac aggaattggc cgacgcccgt gctgaccttg tgcgcatcgc cggtgaaacg
     1441 ttggaaaccg aacgacattt gcaaaacacc ggccaggccg acgaaaccga aatcctcgcc
     1501 gccgaactgg aaacgagccg catgaaactt tccgctcgca cgatggagaa taccttgcgg
     1561 gaagaatggc gggcgctcgc atccgtcctc gggcagcctg atcttcctct gcaaaccgtt
     1621 tccggcgatc tcgagcgcgg ctggccggag ctagacgaac accaggtcga aactgtcgcc
     1681 acgcaaagtc cggccaatcg catcgcttcc gccgcaactg aacgcgccca ggccgaactc
     1741 acgcgcgcac ggcgcgagaa aattcccgac attttggccc gcgccggcct cgactacaat
     1801 cacgaaccac tggacaatcc tctactcagg acgggatggc agtggaacgc tcaacttgcc
     1861 gtcgagctgc caattttcaa tcgcaatcag ggcaacatcg cttctgcgcg cgcaaatatt
     1921 gaccgtgccg aatccgagaa acagcgtgtc tcgctgacgt tgcgcgaacg cgcttcttcc
     1981 gttttcgacg aatacgccaa cgcgcgcctt accgcagagg aataccgcga gaatattctt
     2041 ccgctcgcga agaaatccta caccatgatg tttgaacgct acggcgaaat gctcgcttcc
     2101 tatccgcgcg ttctcgaaaa caagcgcaaa ctttttgaac tgcaaagcga atacatcgct
     2161 tcccttgaaa cggtctggac cactggcctg tcgttacagg gtttcttgct gactgacggg
     2221 ctggaagcgc ctacacagcc gggcgaaatg gaccgcacca tccgcgagac gaacttgccg
     2281 actccagaac gggggcgttt gccgtgagca actgttcatg gtccgaagaa atgtgccttc
     2341 gcggtacatc gcccgtgctg actccgctac actagtacgc tagtcccgtt gacccgtcaa
     2401 attttctctc gctaacgtca acctgggcgc acgggtgtcc acggccatat ccttcggcca
     2461 cccgctgca
//
LOCUS       DASTPM010000714         4225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_689250, whole genome shotgun sequence.
ACCESSION   DASTPM010000714 DASTPM010000000
VERSION     DASTPM010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689250"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1121
                     /locus_tag="VFR42_12645"
     CDS             315..1121
                     /locus_tag="VFR42_12645"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0370058.1"
                     /translation="MARTIAVANQKGGVGKTTTAVNLASCLAAVDQRTLLVDCDSQAN
                     ATAAIGFAKDPSRRTLYHALILNEPTDKIIQKSQVDSLDVVPADKNLAGAAVELVTSE
                     NREYRLQAFLQPLLPQYKFVILDCPPALDLLTLNALVTANSVLVPIQCEYLALEGVSE
                     LLDTLMRIRRTLNPSLEIEGILLTMYDERTTLSRQVATDLRSFFGTQVFQSVIPRNVR
                     LAEAPSFGKPIIFYDMHSKGAEGYTQLAQEVIANAEKRLGTGARSAHSGA"
     gene            1084..1989
                     /locus_tag="VFR42_12650"
     CDS             1084..1989
                     /locus_tag="VFR42_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU0370059.1"
                     /translation="MGRGLGALIREPEPPQPAAENQQPAPVSTGGGAAAVPARESASF
                     GPQEIDIDLIEPSPYQPRTRFREEALEELARSIQASGIIQPIVVRPVGSRFQLIAGER
                     RWRAAQRAGLRKVAAIVRQVADELALEMTLVENIQREDLNAMEAARAFERLMDEFRLT
                     QESVAERTGKDRATVANAIRLLKLEPTIQDWIEEGKLTAGHGRALLAVLDPQLRMRYA
                     QRASRGGLTVRQIERLASRRARGNKERAEAHVDPNVREALEELQRHLGTKIFLRERTK
                     IRPGQLVLEFYDDTQLMGLYDRLMK"
     gene            2143..2562
                     /locus_tag="VFR42_12655"
     CDS             2143..2562
                     /locus_tag="VFR42_12655"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0370060.1"
                     /translation="MAWKWPGSSTSGNSEEWTGFIDQGVTLEGTLQLTGTFRIDGNVK
                     GNIISEQTLILGENAKVEGQIEGNRVVIAGRYDGVIFAKGRVEIQPKGVVTGEVHSPC
                     LVIDPGGIFDGRCHMLAASDTATAPSVTIPIRALSQA"
     gene            2723..4012
                     /gene="purD"
                     /locus_tag="VFR42_12660"
     CDS             2723..4012
                     /gene="purD"
                     /locus_tag="VFR42_12660"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU0370061.1"
                     /translation="MKILAIGGGGREHALVWKLRDSPRAEKIWCAPGNGGIADEATCL
                     PVEAGDAQGLVAIAEKLRPDLTVIGPELPLVNGVSDALRARNLAVVGPSQIGAQLEGS
                     KIFAKNFLLRHSIPTAKMYGEFSRTDEAYSAVPHLPYPLVVKADGLCAGKGVLVAQSA
                     AEAKDFIARAMLIEEFGAGGKRVMLEEALEGDELSFIIVTDGDQYTVLPPTRDYKRVF
                     DGNRGANTGGMGAFSTDDLMSAELRADIEQRIVTPVLQGLTRDGIRYQGFLYVGLMLT
                     IDGPKVLEFNCRLGDPETQAILARTDFDLAEALANLATRRFQPSEWKWKSGASACIVI
                     SSVGYPGKFATGKPIEGLTGIQGQTGVKVLHAGTRRQGDLFLTSGGRVLGVTAYGSDL
                     ASALAKAYAATAQIRFDGMHYRTDIGGGAGRAHSAGD"
     gene            4016..>4225
                     /locus_tag="VFR42_12665"
     CDS             4016..>4225
                     /locus_tag="VFR42_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370062.1"
                     /translation="MPSAANLLRAPKRGLPREELHAAQAVTDRAFAGMFPQAAAGSAP
                     TAAGGCRSSICYRGGSTLRLSVGASG"
BASE COUNT          889 a         1283 c         1220 g          833 t
ORIGIN      
        1 gatgttcgtg aaaacgatat gcgaagttcg gaccggggaa tcagaccccg ccagattagt
       61 tcgtttcggg tgttccacgt ggaacagaat tgcgcagcga agtgtggcgt tgcgttccgc
      121 atgaaccacc aggtgagtcc cttctgcacg gctcaaacgc agcgttccac gtggaacaat
      181 ggcgctccag caagcaagct ctcagaaatt attccacagt tactcgtatg ttccacgtga
      241 aacatcttgc accgtcacct ggactttgct agattttcgc cacatcagtc gtcatgagct
      301 tgcaggagaa cggtttggcg cgaaccattg cggtagcaaa tcaaaaaggt ggagtgggaa
      361 agaccaccac agccgtaaat ctcgcttctt gcctcgccgc cgtcgaccaa cgcacactcc
      421 tcgtcgactg tgactcgcaa gccaacgcca ccgccgcaat cggcttcgcc aaggatcctt
      481 cgcgccgcac tttgtatcac gccctaattc tcaacgaacc gacagacaag attattcaga
      541 agtcgcaagt ggactcgctc gatgtcgtcc ctgccgacaa aaacctcgcc ggagccgcgg
      601 tcgaactggt tacttccgaa aatcgcgaat atcgcctgca agcctttttg cagcctcttt
      661 tgccgcagta caagtttgtg attctggatt gtccgccagc tctggacctc ttgacgctga
      721 atgccctcgt gaccgccaat tccgtgctgg tgcccatcca atgcgaatat ctggcacttg
      781 agggagtctc cgaattgctg gacaccttga tgcgcattcg ccgcacgctc aatcccagcc
      841 tggagatcga aggcattctc ctaacgatgt acgatgagcg gaccacactt tcgcggcagg
      901 ttgccaccga cttgcgcagt tttttcggaa cgcaggtgtt ccaaagcgtg attccgcgca
      961 atgtacgcct ggccgaagct cctagttttg gtaaacctat tattttctat gacatgcata
     1021 gtaagggggc ggagggctat acgcagctcg cccaggaggt aattgctaat gccgagaaac
     1081 gccttgggac gggggctcgg agcgctcatt cgggagcctg agccacccca gccggcggca
     1141 gaaaatcagc agccggcacc agtctcgaca ggtgggggag ccgccgccgt tcctgcgcgg
     1201 gaaagcgcca gcttcggtcc gcaggaaatt gacatcgacc tcatcgagcc aagcccgtac
     1261 cagcctcgca ctcgcttccg cgaggaggct ctggaagaac tggcccgctc cattcaagcc
     1321 agcgggatta ttcagccgat cgtcgtacgg ccggtaggca gccgcttcca gttgatcgcc
     1381 ggtgaacgcc gctggcgcgc ggcccagcgc gctggcttgc gcaaagttgc ggctatcgtt
     1441 cgacaggtag ccgacgaact ggcgctcgaa atgaccttgg tcgaaaacat ccagcgtgag
     1501 gatttaaacg cgatggaagc tgcccgtgcc ttcgaacgat tgatggatga atttcggctg
     1561 acccaggaat ccgtcgcgga gcgcaccggc aaagaccggg cgaccgtagc aaacgctatc
     1621 cgtttgctca agctcgagcc gacgatccag gactggattg aggaagggaa gctaaccgcc
     1681 ggccacggcc gggcacttct tgcagtgctc gatccgcagc tgcggatgcg ctacgcccaa
     1741 cgcgcttctc gtggcggctt gacggtgcga cagatcgaac gcctggcctc acgtcgagcg
     1801 cgcgggaaca aagaaagggc ggaagctcac gtcgatccca atgtccgcga agcgctggaa
     1861 gagctccagc ggcatctcgg caccaagatt tttctgcgcg aacgcaccaa gattcgcccc
     1921 ggacagctgg tgctggaatt ctacgacgac actcagctga tgggacttta cgatcgattg
     1981 atgaagtaag agagatttcc gcgcgaaggc agggaactgt ccctgcgttc agtcgcacat
     2041 tcgacacctc ttcagaattt ttcgcagaac agtcttggac tcgacaaaac gagtcgctac
     2101 agtgagagcg tcgatttcgt ctggcgacct gaggatgctc aaatggcgtg gaagtggccc
     2161 ggcagttcaa ccagcggtaa cagcgaagag tggaccggtt tcatcgatca gggtgtgacg
     2221 cttgaaggca cgctgcagct caccggcacg tttcgcatcg atggtaacgt taaaggtaat
     2281 attatttctg aacaaacatt gatcctgggt gagaacgcca aggtcgaagg gcaaatcgaa
     2341 ggcaatcgcg tggtgattgc cggccgctac gacggcgtca tctttgcgaa gggccgcgtg
     2401 gaaattcaac ccaagggcgt cgtcaccggc gaagttcact cgccctgtct ggtcatcgat
     2461 cccggcggaa tttttgacgg gcgctgccac atgctggccg cttcggacac tgcgaccgct
     2521 ccgagcgtca cgattccgat tcgcgcactc agccaggcat agcccctcat tctttcattt
     2581 gccttgaaat catagaagct cgccacgcgc gcaaatcgtc gcccggtgcc ttctagccaa
     2641 agtcgctaca ggattgacac ctgctcggcc cgcgtctatc gtcgagtagg aaattttcgc
     2701 ccaaggaaag tttcgcgcct tcatgaaaat tctggccatt ggcggcggcg gccgcgagca
     2761 cgcgctggtc tggaagctgc gcgatagccc gcgcgccgaa aagatctggt gcgcccccgg
     2821 caacggcggc atcgctgacg aagctacctg tttgcccgtc gaagcgggcg acgcgcaagg
     2881 cctggtcgcc atcgcggaaa agcttcgtcc ggacctcacc gtcatcggcc cggagctccc
     2941 gctggtgaac ggagtaagcg acgccctgcg cgcgcgcaat ctcgcggtcg tcggaccctc
     3001 gcaaatcggc gcgcagcttg agggcagcaa aatcttcgcg aaaaacttct tgcttcgcca
     3061 ttccattccg accgccaaga tgtatggcga attttcccgc accgacgaag cctacagcgc
     3121 tgttccgcat ttgccgtacc cgctcgttgt caaagccgat ggcctgtgcg ccgggaaagg
     3181 cgtcctggtt gcccagtccg ccgccgaagc caaggatttc atcgcgcgcg ccatgcttat
     3241 cgaagagttc ggtgcgggcg gcaagcgcgt tatgttggaa gaggcgctcg aaggcgacga
     3301 actctccttc atcatcgtca ccgacggcga ccaatacaca gtgcttccgc caactcgcga
     3361 ttacaaacgc gtctttgatg gcaaccgcgg tgccaacacg ggcggcatgg gggcgttttc
     3421 caccgacgac ctgatgtcgg ccgaacttcg cgccgatatc gaacagagga tcgtcacacc
     3481 cgttctgcaa ggcctcaccc gcgacggcat ccgctaccag ggcttcctat acgtcgggct
     3541 gatgctgacc attgacgggc caaaagtatt ggaattcaat tgccggttag gggaccccga
     3601 aactcaggca attctggcgc ggaccgactt tgatctagcc gaagcgctgg cgaatttggc
     3661 aacccggcgg ttccagccga gcgaatggaa atggaagtcc ggcgcaagtg catgcattgt
     3721 aataagttcc gtcggctatc cgggcaagtt cgccaccggg aagccaattg aaggactaac
     3781 cggtatacag ggccagaccg gtgtgaaggt tcttcatgcg ggcacccgcc gccaaggcga
     3841 cctcttcctg accagcggcg gccgtgtcct cggcgtcacc gcctatggtt ccgatctggc
     3901 cagcgcgctc gccaaagcct atgccgccac agcgcaaatt cgctttgatg gcatgcacta
     3961 ccgcacagat attggcggcg gtgccggtcg ggcgcattca gcgggagact agcctgtgcc
     4021 ctccgccgcc aacctgcttc gcgctcccaa gcgcgggcta ccgagggagg agctgcacgc
     4081 cgcgcaagcc gtcaccgatc gggcattcgc cggaatgttc ccacaggctg cggcagggag
     4141 cgcgccaact gctgctggtg gatgccgttc ctcaatctgt tatcgcggcg gatcgactct
     4201 gcgcctcagc gtcggtgctt ccggc
//
LOCUS       DASTPM010000715         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1064443, whole genome shotgun sequence.
ACCESSION   DASTPM010000715 DASTPM010000000
VERSION     DASTPM010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064443"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..273)
                     /gene="rpmA"
                     /locus_tag="VFR42_12670"
     CDS             complement(13..273)
                     /gene="rpmA"
                     /locus_tag="VFR42_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU0370063.1"
                     /translation="MAHKKGGGSSTNGRDSHGQRLGVKRFGGQLVNAGTILVRQRGTK
                     HQAGENVGQGKDDTLFALVTGHVKFEDKGRRGRFISVIPTAN"
     gene            complement(369..680)
                     /gene="rplU"
                     /locus_tag="VFR42_12675"
     CDS             complement(369..680)
                     /gene="rplU"
                     /locus_tag="VFR42_12675"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU0370064.1"
                     /translation="MYAVIKSGGKQYRVAPGDTIRVERVSGEVGEQVKFGTVLAVHTD
                     DKKVLVGDDASKASVTGKIVRQGRSPKVTVLKYKKTNQYKIQRGHRQNFTAVEVSDIQ
                     L"
     gene            complement(772..2541)
                     /locus_tag="VFR42_12680"
     CDS             complement(772..2541)
                     /locus_tag="VFR42_12680"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU0370065.1"
                     /translation="MPRRSASLRLIVCFLLFAISTFAYSAFPDADSWSHLSDDFIDQY
                     VAFSPTASTQVGIHIHDAELDDYSPASMAKQIAWLQQFEKRVLAFNAKTLSVTDAADR
                     EILLNNIRANLLELQVIRTFAKNPDNYSSGAAASIYVIMIRKFAPPDERLRSVIAREK
                     QFPRFFADARANLQVNLKNVPRIYTEIALEQLPGTISFFEKDVPSAFADATDPAVKSA
                     FAESNAAAIKLLREYQEWLKSEVLPRSNGDFRIGADNFSRKLLYDDMVSTPLDRLLEI
                     GMADLHKNQSEFQRVAKEVDPSKSPAEVLAELQSMHPAPSEVLQSFRNTFDGLIAFIS
                     MHHIITLPADRRPTLQETPPFERATTTASMDTPGPFEKVATDAYFNVTLPQSGDSPED
                     VAGIMAGLNVGTIISTSIHEVFPGHFTQYLWEQRAPTKLRRILSANTNVEGWAHYTEQ
                     MMLDEGYAQPDAGAKNLREARLIRLGQLQDALLRDARFVAGIQMHRGKFTLDQAREFF
                     VKEGFQSPRIADIETKRGTSDPTYLYYTLGKLQILKLREDLRQKQGAAFSLGKFHDDF
                     VSQGYPPIAIVRRALLGDSSRTL"
     gene            2672..3199
                     /gene="hslV"
                     /locus_tag="VFR42_12685"
     CDS             2672..3199
                     /gene="hslV"
                     /locus_tag="VFR42_12685"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007302170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEU0370066.1"
                     /translation="MHGTTVLCVRKEGKVVLLADGQVTMGDHVMKHTARKTRRLYQDK
                     VIAGFAGSTADAISLFERFEGKLQEFSGNLPKAAVELAKEWRKDRALRHLEALLIVAD
                     SKGTFILSGNGDVIEPDDGIVAIGSGGSYALAAARAMMKHSKLGAKDLAMEAMRIASE
                     ICIYTNANFTVEELG"
     gene            3243..>4535
                     /gene="hslU"
                     /locus_tag="VFR42_12690"
     CDS             3243..>4535
                     /gene="hslU"
                     /locus_tag="VFR42_12690"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: protein motif:HMM:NF003544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU0370067.1"
                     /translation="MASKSDKELVLVQGGANGAQPEGEEFLPALDDMTPREIVSELDK
                     YIVGQAAAKRAIAVALRNRVRRQKLPPELSDDILPKNILMIGPTGVGKTEIARRLARL
                     AGCPFVKVEASKYTEVGYVGRDVESMVRDLVEMSIDMVREEKLDEVAERAEQAAEERV
                     LDLLLPPPPPAGPGTTESDAASQREQNQRTRDKLRAQLREGKLDQRMVDLEVRERMMP
                     SFEVISNQGMEEMDVNFKEMLSGMFGQQKKKRKMSVADAFEYLIQEEEGKLVDMDQVT
                     RIAVERAEQMGIIFIDEIDKVAGRESGHGPDVSREGVQRDILPIIEGTTVNTRYGMIR
                     TDHILFVAAGAFHVTKPSDLIPELQGRLPIRVELQSLKEEDFVRILTEPKNALIKQYI
                     ALLETEGLKLNFTDDAVETIAKFATQVNEQTENIGARRL"
BASE COUNT         1003 a         1118 c         1512 g          902 t
ORIGIN      
        1 cctacacaaa acctaattcg ctgtggggat gacgctgatg aaacgtccgc gacggccctt
       61 gtcttcgaac ttcacgtggc cggtgacgag cgcgaacagc gtgtcgtcct tgccctgccc
      121 gacgttttcg ccggcctggt gcttggtgcc gcgctggcga accagaatcg tgccggcatt
      181 gacaagctgt ccgccgaagc gctttacgcc gagccgctga ccgtgagaat cgcggccgtt
      241 tgttgaggag ccgccacctt ttttgtgtgc cattgctagt tccttttcaa aagcgaatat
      301 tgcgagagct gcgtgcggtt cctgccaaga cgcagccacg ctgggctaac gcccagagct
      361 caacaagcct acagctggat gtcgctgact tcgacggcgg tgaagttctg gcgatgaccg
      421 cgctgaatct tatactggtt ggttttcttg tacttcaaaa cggtgacctt ggggctgcgg
      481 ccctgacgga cgatcttgcc ggtcacgctg gctttcgaag cgtcgtcgcc aaccaaaacc
      541 tttttgtcgt cggtgtgcac cgccagaaca gtgccgaatt tgacctgttc gccgacttca
      601 ccggaaactc gttccacacg aatagtgtcg ccgggagcga cacggtactg ctttcccccg
      661 gacttgataa cggcgtacat cactcctcca agagctaaag acaaacccta attatactgg
      721 tcgcagagag gaaccgtcaa cgacaggttt acggtgcttc cttggacgtg cctagagggt
      781 gcgcgaggag tcgccaagca gggcgcggcg gacgatggcg atgggcgggt agccctggga
      841 cacgaagtcg tcgtggaatt tgcctaggga gaaggcggca ccttgttttt gacgcaagtc
      901 ttcgcggagc ttgagaatct gtagtttgcc gagcgtgtag tagagatagg tggggtcgga
      961 agtgccgcgc ttggtttcaa tgtcggcgat gcggggcgac tgaaaaccct ccttgacgaa
     1021 gaattcgcgc gcttgatcga gggtgaattt gccgcggtgc atctggatac cggctacgaa
     1081 gcgagcgtcg cgcaggaggg cgtcctgcaa ctgacccagg cgaatgaggc gcgcttcgcg
     1141 cagatttttg gcgccggcgt cgggctgcgc atagccttcg tcgagcatca tctgctcggt
     1201 gtaatgggcc cagccttcga cgttggtgtt ggcggagagg atgcggcgca gcttagtggg
     1261 ggcgcgctgt tcccaaagat attgcgtgaa gtggccgggg aaaacctcgt ggatggaagt
     1321 gctgatgatg gtgccgacat tgaggccagc catgatgccg gcgacgtctt cgggcgagtc
     1381 acccgactgc gggagggtga cgttgaaata ggcgtcggtt gcgacttttt caaacgggcc
     1441 gggcgtgtcc atggaagcag tagtggtagc gcgctcgaac ggcggcgttt cctgcaaggt
     1501 gggccggcgg tcggcgggca gcgtaatgat gtggtgcatg ctgatgaaag cgatcagccc
     1561 gtcaaaggta ttgcggaagc tctgcaggac ctccgaggga gcgggatgca tggattgcaa
     1621 ttcggcgaga acttcggcgg gcgatttgga gggatcgact tctttggcca cacgctggaa
     1681 ctcggactgg tttttgtgga gatcggccat gccgatttcg agtaggcgat cgagcggcgt
     1741 ggaaaccatg tcgtcataga ggagttttct ggagaagttg tcggcaccga tgcggaagtc
     1801 gccgttggag cgggggagca cttcggactt gagccactcc tgatattcgc ggagaagttt
     1861 gatggcagcg gcgttggatt cggcgaaggc ggatttaacg gcgggatcgg tggcgtcggc
     1921 gaaagcggaa ggcacatctt tttcgaagaa gctgatggtg ccgggaagct gttcaagagc
     1981 gatttctgtg tagatgcgcg ggacattctt gagattgacc tggagattgg cgcgagcgtc
     2041 ggcgaaaaag cgtggaaact gtttttcgcg ggcgatgacg gagcgcaggc gttcatcggg
     2101 cggcgcgaac ttgcggatca tgatgacgta aatgctggcc gcggcgccgc tcgagtagtt
     2161 gtcgggattt ttggcgaagg ttcgaatgac ctgaagttcg agcagattgg cgcgaatatt
     2221 gttgagaagg atttcacggt cagcggcgtc ggtgacgctg agcgttttcg cgttaaacgc
     2281 aagcacgcgc ttttcgaatt gctgcagcca ggcaatttgt ttggccatgg aagcgggcga
     2341 gtagtcgtcc agctcggcgt catgaatgtg gatgccgacc tgcgtggaag cggtcgggct
     2401 gaaagccaca tactggtcga tgaagtcgtc agatagatgg ctccaggaat ccgcatccgg
     2461 aaaagcagaa taggcgaacg tggaaatggc gaacagaagg aaacagacga taagcctcag
     2521 agacgctgac ctgcgcggca tgatcacctc agaagaaatg cgaaaaagta gatgtaagag
     2581 tacaagagta tgaggttttt gcgcctcccg cggcgcggcg tataatggtg gaatgccaat
     2641 gagccaaaat agtgcgcatg gggccggtgt aatgcacgga accacggtat tgtgcgtgcg
     2701 caaagagggc aaagtggtgc tgctggccga tggccaggtg accatgggcg atcacgtgat
     2761 gaagcacacg gcgcgcaaga cgcgcaggct ctatcaggac aaagtgattg cgggattcgc
     2821 cggctccacg gccgatgcca tttcactttt cgaacggttc gaaggaaagc tgcaggaatt
     2881 ttccggcaat ttgccgaagg ccgcggtgga actagctaaa gaatggcgca aggaccgcgc
     2941 cctgcggcac ctggaggctt tgctgatcgt ggccgacagc aagggcacat ttattttgtc
     3001 cggcaacggc gatgtgattg agccagacga cggcatcgtg gcgatcggct ctggcggatc
     3061 gtatgcgctt gcagcggcgc gcgcgatgat gaaacactca aagttgggcg ccaaggattt
     3121 ggcgatggag gcgatgcgca tcgccagcga aatttgcatc tacacgaacg cgaactttac
     3181 cgtcgaagaa ttgggctagg ccggcaacta caaactgaga acggaccact gaaaactgtc
     3241 ttatggcaag caagtctgat aaggaattgg ttctggtgca aggcggagcg aacggcgcgc
     3301 agccggaagg cgaagagttt ctgcccgcgc tggacgacat gacgccgcgc gaaatcgtgt
     3361 cggagctgga caaatatatt gtcgggcagg cggcggcgaa gcgcgcgatc gcggtggcgc
     3421 tgcgcaatcg agtgcggcgg cagaagctgc ctcccgaact ttccgatgac attttgccga
     3481 agaatatttt gatgatcggg ccgaccggcg taggcaagac ggagattgcg cggcggctgg
     3541 cgcgattggc aggctgtccg ttcgtaaaag tggaagcgtc gaaatatacg gaagtgggct
     3601 acgtcgggcg cgacgtggaa tcgatggtgc gcgacctggt ggaaatgtcc atcgacatgg
     3661 tgcgcgaaga gaagttggat gaagttgcgg agcgcgccga gcaagccgcg gaagagcgcg
     3721 tgctggatct gctgctgccc ccaccccctc ccgcgggacc gggaacgacg gaaagcgatg
     3781 ccgcttcgca gcgtgaacag aaccagcgca cacgcgacaa gctgcgcgca cagcttcgcg
     3841 aaggcaaact cgatcaacgg atggtggacc tggaagtccg cgaacgcatg atgccgtcgt
     3901 ttgaagtcat ttccaaccag ggcatggagg agatggacgt caatttcaag gagatgctga
     3961 gcgggatgtt cggccagcaa aagaagaaac gcaaaatgag cgtggccgac gccttcgaat
     4021 acctgatcca ggaagaagaa ggcaagctgg tggacatgga tcaggtgacg cgcatagcgg
     4081 tcgagcgcgc cgagcagatg gggattattt tcatcgatga aatcgacaag gtggcggggc
     4141 gcgagtccgg tcacgggccg gacgttagcc gcgaaggggt gcagcgcgat attttgccaa
     4201 ttatcgaagg cacgacggtg aatacacgct acggcatgat tcgcaccgac catattttgt
     4261 ttgtggcggc gggcgcgttt cacgtaacca agccttccga cctgattccc gaactgcaag
     4321 gtcgcctgcc gattcgcgtg gagctgcaat cgctgaagga agaagatttc gtgcgcattc
     4381 tcaccgaacc caagaatgcg ctcatcaagc agtacattgc attgctggaa acagaagggc
     4441 tgaagctgaa tttcacggat gacgcggtgg aaaccatcgc aaagttcgcc acccaagtga
     4501 acgagcagac cgaaaacatc ggcgcacggc ggcta
//
LOCUS       DASTPM010000716         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1035380, whole genome shotgun sequence.
ACCESSION   DASTPM010000716 DASTPM010000000
VERSION     DASTPM010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035380"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="VFR42_12695"
     CDS             <1..1921
                     /locus_tag="VFR42_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370068.1"
                     /translation="RRALFGEDFAPYYDLLKNRLIFLDGGKDDSYFLEHFVLLGNYVR
                     DPDRFEAMDALFQEFLQQVGVAIAHDPDFVEAQREHQGILDQVQAMRTEIANLEEQRE
                     QHRKKLDRGDGFLTKFLNSGDPANIKASLNDIELRLKHQQLKLEEFGPQVEAAKQKLD
                     FFLKGHHGQLGSYLNEPKNAAKLFDPAAEPEALRAQRTQLLTRFMERMEQQEVLYHVL
                     AGYEIRSIAAQYHPPVHLQQLRKALVSKDELKRVEQVLKQVPAKQLSAKPIEELGRKI
                     RRYSRDETQALVLKFATDFLRLRRELRDAEHLTTCMERVNLVSTEQARDLSRLNNRLY
                     ECVLQDEAKPQQDPVVSHVIIKADVRGSTRMTQDLLTRGLNPASHFSLNLHEPVKKLL
                     DRYSAKKVFLEGDAIILAIFETESSRAYARPVAKACVLARQILAVCNSYNDSAAKTDL
                     PALELGLGVAFQGSAPAYWTDGDSRIMISKALNLSDRLSGCTKLARRMLAEQKSNLSI
                     FHFLQTMQGASAEELDEFLVRYNRNGIELNDEGFQKLSEEISLESVETKLDLPWGKEK
                     VTLYYGEVPIGDSVELLVLRKGAARELSSDGKIGKSSEHLYYEVCTAPALFNLVAALI
                     RTTQVSVPVPVHAGR"
     gene            2393..2491
                     /locus_tag="VFR42_12700"
     tRNA            2393..2491
                     /locus_tag="VFR42_12700"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2427..2429,aa:Sec,seq:tca)
BASE COUNT          577 a          650 c          809 g          554 t
ORIGIN      
        1 gcggcgagct ctgtttggcg aagattttgc gccgtactac gatttgttga agaaccggct
       61 gatttttctg gatggcggga aggacgattc atatttcctg gagcattttg tactgctggg
      121 aaattacgtg cgcgacccgg accggttcga agcgatggac gcgctgttcc aggaattttt
      181 gcagcaggtg ggagtggcga ttgcgcacga tccggatttt gtggaagcac aacgggagca
      241 ccagggaatc ctcgaccagg tgcaagcgat gcgtacggag attgccaatc tagaagagca
      301 gcgcgagcag caccgcaaga agctggaccg cggcgatgga ttcttgacga agttcctgaa
      361 ttcgggggac ccggcgaaca ttaaagcttc gctgaacgac attgaacttc ggctgaagca
      421 tcagcagctg aagctggaag aattcgggcc gcaagtagaa gcggccaagc agaagctgga
      481 tttctttctg aaagggcacc acgggcagct cggaagctat ctgaacgagc cgaaaaatgc
      541 ggccaagctg tttgaccccg cagcggagcc ggaggcgctg cgcgcccagc gaacacaact
      601 gcttacgcgg ttcatggagc ggatggaaca gcaggaagtg ctgtatcacg ttctggccgg
      661 ctacgaaatt cggagcatcg cagcgcagta tcatccgccg gtgcacctgc agcaattgcg
      721 aaaagcgctg gtttcgaaag acgagttgaa gcgtgtggag caggtgctga agcaggtacc
      781 cgcgaagcag ctttccgcaa aaccgatcga agagttggga aggaaaattc gacgctactc
      841 gcgggacgag acgcaggcgc tggtcttgaa gttcgcaacg gactttttgc gtttgcggcg
      901 ggaacttcgc gacgcggaac atctgacgac gtgcatggag cgcgtgaacc tggtttctac
      961 agaacaagcg cgtgatttgt cgcggctgaa caaccggctg tatgagtgtg tgttacagga
     1021 cgaggccaag ccgcagcagg atccggtggt ttcacacgta attatcaagg ccgacgtgcg
     1081 cgggtcgacg cggatgacgc aggacttgct gacgcgaggg ttgaatccgg cgtcgcactt
     1141 cagcttgaac ctgcatgagc cggtgaaaaa gctgctggac cggtattcgg cgaagaaagt
     1201 gttcctcgag ggcgacgcaa ttattctggc gatttttgag acggaatcga gccgggcgta
     1261 tgcgcggccg gtggccaagg cttgtgtgct ggcgcggcag attctggcgg tgtgcaattc
     1321 gtacaacgac agcgcggcga aaacggactt gccggcgctg gaactggggc tcggcgtggc
     1381 gtttcaaggg tcggcgccgg cgtattggac ggacggcgac tcgcgcatca tgatttccaa
     1441 agcgctgaat ctttccgatc gtttgtcggg gtgcacgaaa ctggcgcgcc gcatgctggc
     1501 cgagcagaag tcgaatcttt cgatctttca ttttttgcag accatgcagg gtgcgtcggc
     1561 ggaagaactg gatgagttcc tggtgcgata taaccgcaac ggaatcgaat tgaatgacga
     1621 aggatttcag aagctatcgg aggagatttc gctggaatcg gtggaaacca agctcgactt
     1681 accgtggggc aaggagaagg tgacgttgta ctacggagaa gtgccgatcg gggacagtgt
     1741 ggagctgctg gtgctgcgca aaggcgcggc gcgggaattg tcttcggatg gaaaaatcgg
     1801 caaaagctca gagcatctgt actacgaggt atgcacggcg ccagcgctgt tcaatctggt
     1861 ggcggcgctg attcgaacga cgcaggtttc cgtgcctgtg cccgtgcacg cggggcgata
     1921 gagcctttcc caaaacaatt cgcagctgaa tttgccggct tggaattggc gggaacggta
     1981 acgtttctct ttgaaccgga gtcccagacc agacactcaa tgaacacttc acaatcgcag
     2041 gatacgcatg actgacgttt cccccttttc tatttaggga gaatcctttt tgaaacggga
     2101 gacgattacg ccgatggcac aaatccgatg gattactttg ttgtacggct gatcgctatt
     2161 ctttcctgac ggcttttttg caaggacgag gcgcgtttct gagctccggt gagagcagga
     2221 gcacttcgaa cggctcaccc cctgccgtgg ccgggcttca tgcccagcct gtccgcgcac
     2281 ctgtccatct caagccttca cgagacggtt tcccggataa cggacagcac cgcagccgat
     2341 tttcaggctc cctatgcttc gtatatccac taagtccctg taaaatcact gtggaggcga
     2401 aaggggtccg gtgaccttcc cggccttcaa agccggcgat tcggctctta gcgagccgag
     2461 tggtgagttc gactctcaca cgcttccgcc aagctccttg tttttcaagt agatttagcc
     2521 ttaactgact ccaattgagc caggttgtgc ccgatcttcc gggagaattt ctcggaagcg
     2581 acacgcgcgt
//
LOCUS       DASTPM010000717         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_26260, whole genome shotgun sequence.
ACCESSION   DASTPM010000717 DASTPM010000000
VERSION     DASTPM010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26260"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1893)
                     /locus_tag="VFR42_12705"
     CDS             complement(280..1893)
                     /locus_tag="VFR42_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEU0370069.1"
                     /translation="MVVFMKPPKCLNCMANLASATIGAPSAAPGGFVLDSYSSAVAEF
                     GGKVAYRDLREFVKALEKAGELKRVRAEVDPVLEIAEVAQRVARDVNRKPQSVGPALL
                     FEKPKGSRYPLLINTFGSVQRMRLAFEVDELDEVAARIRGFLDTKSPAGLFDKLKMLP
                     KLAELGSFFPKSVKDGDCKEVIHKGGDVSLFDFPILQCWPQDGGRFITFPLVFTKNPE
                     TGKRNVGMYRMQVYDERTTGMHWQTQKHGAEHFRRARAKNPEGRIPVSVAIGSDPATT
                     LAGMLPIPPDLDEMMFAGFLRSSPVEMVRCETNELEVPANAEIVLEGYVNLNEFRTEG
                     PFGDHTGFYSLEGEYPVFHVECITRRKDPIYLTTVVGPPPQEDFYMGYAVERVFLPVM
                     KMQYPEIVDVAMPAEGIFHNLMIVAIRKSYPGHARKIMNAIWSLGQAMFTKVIVVVDH
                     DVDVHNYSEVVWKALCALDPERDVQFSLGPVDTLDHAQRMQDYGSKMGIDATRKWLTE
                     GFSRPWPDEILMDEATKKRVASLWSSLGL"
     gene            1880..2329
                     /locus_tag="VFR42_12710"
     CDS             1880..2329
                     /locus_tag="VFR42_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370070.1"
                     /translation="MKTTILALLAMVVAPSLFAQGAPPQGAADKPYIMEYYYKVSWGH
                     QKEFLDLFRKNHFPLLQEIQKSGRILALKIETPGNHMTEESRWDYRVTITYKNSTLAT
                     TSNPDEEALVKKLYPDRALFEREEQRRFEILLAHWDLPVSDITAAQK"
     gene            2352..>2664
                     /locus_tag="VFR42_12715"
     CDS             2352..>2664
                     /locus_tag="VFR42_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU0370071.1"
                     /translation="MNIILFGATGMVGQGVLRECLRDPAVRRVVTIGRTPTSVQNAKQ
                     QDIVHPDLFDYADIEPQLTGFDACFFCLGVASSGMNETAYHRITYELTLAAAQTLAHL
                     NP"
BASE COUNT          589 a          872 c          662 g          541 t
ORIGIN      
        1 aagccggtat caaaatcgca gaccgttgaa acgccgacgc gccagaaacc gctggcacgg
       61 ggaacgatta tgtcgggaac cgtagcgatt acgcgagcct cgttcgcctt ggttgccgtt
      121 tgcggagatc gtgcattcac aatagggaca tagcgaagca gattagaagg atcgagatat
      181 gggcggtgtc gctcagtggg taagcgggac ggtcacgtgg gcagatctgg attctccgct
      241 ggctccctcg gtcaccgcgg accgccgggc ccttagtggt cagaggccca gggaggacca
      301 gagggaagcc acgcgcttct tcgtcgcctc gtccatcaaa atttcgtccg gccacggccg
      361 cgaaaatcct tctgtgagcc acttgcgcgt cgcatcgatg cccattttgg agccgtagtc
      421 ctgcatgcgc tgcgcgtggt cgagcgtgtc cacgggtccc agcgaaaatt gcacgtcgcg
      481 ttccgggtcc agcgcgcaca acgccttcca caccacttcg ctgtagttgt gtacgtccac
      541 gtcgtgatcc accaccacaa tcactttggt gaacatggcc tgacccagcg accagatggc
      601 gttcatgatt ttgcgcgcgt gccccggata cgacttgcga atcgccacga tcatcaggtt
      661 gtgaaaaatt ccttccgccg gcatcgccac gtccacaatt tccggatact gcattttcat
      721 caccggtaga aacacgcgct ccaccgcgta gcccatgtag aaatcttcct gagggggcgg
      781 ccctaccacc gtcgtcaggt aaatcggatc cttgcggcgc gtgatgcact cgacatgaaa
      841 caccggatac tcgccctcta gcgaatagaa tcccgtgtgg tcgccgaacg gcccttccgt
      901 gcggaactca ttcaaattca cgtagccttc cagcacaatc tccgcattcg ccggaacttc
      961 cagttcgttg gtttcgcacc gcaccatttc taccggcgaa ctgcgtagaa atcccgcgaa
     1021 catcatttcg tccagatcgg gcggaatcgg caacatcccc gccagggtcg ttgcaggatc
     1081 gcttccaatc gccacgctca ccggaattct tccctctgga ttttttgctc tcgcgcgccg
     1141 aaaatgctcc gcgccgtgct tttgcgtctg ccagtgcatg cctgtcgttc gttcgtcgta
     1201 cacctgcatg cgatacatcc cgacgtttcg cttcccggtc tccggattct tcgtgaacac
     1261 cagcggaaac gtaatgaatc gcccgccatc ctgcggccaa cactgcaaaa tcggaaaatc
     1321 gaaaaggctg acatcgccgc ccttgtgaat cacttcctta caatcgccat ctttcacgct
     1381 cttcggaaaa aacgaaccca actccgccaa cttcggcaac atcttcagct tgtcgaacag
     1441 cccagccggc gacttcgtat ccagaaatcc gcgaatccgc gctgccactt cgtccaattc
     1501 gtccacttcg aacgccagac gcatgcgctg aacgctgcca aacgtgttga tcagcagcgg
     1561 atagcgcgag cctttgggct tctcaaacag cagcgccgga cctacgctct gcggcttgcg
     1621 attcacatcg cgcgccacgc gttgcgctac ttcggcaatc tccaggaccg gatccacttc
     1681 tgcgcgcacc cgtttcagct cgcccgcctt ctcgagcgcc ttcacaaact cccgcaaatc
     1741 ccgatacgcc actttgcctc cgaattccgc taccgcactg gagtaagaat ctagcacgaa
     1801 gccgccgggt gcagcggaag gggcgccaat tgtcgcgctg gccagattgg ccatacaatt
     1861 cagacacttg ggaggcttca tgaaaaccac cattcttgcc ctattagcga tggtagtggc
     1921 gccatcgcta tttgcccaag gcgcgccacc gcaaggcgcg gccgacaagc cctacatcat
     1981 ggagtactac tacaaagtct cctggggcca ccaaaaggaa ttcctcgacc tcttccgcaa
     2041 gaaccatttc ccgctgctgc aggaaattca aaaatccggc cgcatcctag cgctgaaaat
     2101 cgaaacgccc ggcaaccaca tgaccgaaga atcccgctgg gactatcgcg tcaccatcac
     2161 gtacaaaaat tcaacactcg ccaccacttc caatcctgac gaggaagcgc tggtcaaaaa
     2221 actctatccc gatcgcgcac tcttcgagcg cgaagagcag cgccgcttcg aaatcctcct
     2281 ggcacactgg gacctgcccg tctccgacat cacagcggcc cagaaataaa gcagcactga
     2341 gcttcgacag gatgaacatc atcctcttcg gcgcgaccgg catggtggga caaggcgtct
     2401 tgcgtgaatg cctgcgagac cccgcagttc ggcgggtagt gacgatcggg cgcaccccaa
     2461 ccagcgtgca aaatgccaag cagcaggata tcgtccatcc ggacctgttc gattacgccg
     2521 acatcgaacc acaactcacc ggtttcgatg cctgcttttt ctgcctgggc gtcgcttctt
     2581 caggaatgaa cgaaaccgct taccatcgca taacctacga acttacactg gccgccgcac
     2641 aaacgctggc gcatttgaat ccgc
//
LOCUS       DASTPM010000718         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_112525, whole genome shotgun sequence.
ACCESSION   DASTPM010000718 DASTPM010000000
VERSION     DASTPM010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112525"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..405
                     /locus_tag="VFR42_12720"
     CDS             43..405
                     /locus_tag="VFR42_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370072.1"
                     /translation="MTLFSSWFWPDVSTRKNATFATTEAFYVLLGVAILVALFTLVDF
                     ARGWEFLDRIAQVAVTGHPGALLLAIAVALALLHRVRGAFAFHKFAAIPAHTPSIEQS
                     FRSFGQNSPRSEKDTGRN"
     gene            415..1695
                     /gene="dinB"
                     /locus_tag="VFR42_12725"
     CDS             415..1695
                     /gene="dinB"
                     /locus_tag="VFR42_12725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU0370073.1"
                     /translation="MPKLAQFRAKHDDASALTLPAGSTILHVDMDAFFVSVELLERPE
                     LRGKPVVVGGRPDQRGVVSAASYEARKFGIQSAMPLRTAARLCPHAIFLDGHHDKYGE
                     FSDRVATILAQFSPIVEMVSIDEAYLDLAGTERLHGPPFAAAHQLLRMITSETSLPCS
                     AGLASTRLVAKVASDQAKPKGLLWIPAGQESRFLAPLPVRKIPGIGEVTERALRALGI
                     EYVHQLADAGLDKLEKIFGQWGTALYRKACGGDSYEFVLDAEPKSISHNHTFGEDTDD
                     TPVLESMLSHLSQKACKRLREAGLSARTLTLTIRYAGFDTYTRAKTPPDPVQFDADAF
                     AQFLALFREQRNTKRKIRLLGVAFSGLIHGNAQLDLLDPQRRAKLEKLSRAADSLRDR
                     FGFAKVQFGGSLLAERPERENKKGEIPRFPRNKE"
     gene            1759..2937
                     /locus_tag="VFR42_12730"
     CDS             1759..2937
                     /locus_tag="VFR42_12730"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEU0370074.1"
                     /translation="MPSTAPPPAPPPEYTRQPRRVPGDGIWDRISEGRRLDELWSQFS
                     ADAQASYGFYGKDLDWEQLKTIPAWRRPFRVAKHFFWALVMKMTPARRVLLFVALAML
                     ILSSPKLNVGHSFSFDVNLDAIAALLFLLLLSLELADKVTMKRDLEIAREIQSWLTPS
                     QPPPVTGASIAFHTRPQNSVAGDYYDAFYPLQDAESGGKLFLVIADVAGKSVPAALLM
                     ATFQASLHTVAREGVSLQKLVARLNRYACAHSLDGRRFTTAMLGEYDPATRIFTYVNA
                     GHNAPVLRRYNANGEAQIERLPASGVPFGISLDANYEIATVQLAPRDALVLFTDGVVE
                     AFNAKGEEFTDARWVDTVRHLPDWEAQRALQFLMQRVDEFVGATKQFDDITCLVVRCE
                     "
     gene            3009..>3628
                     /locus_tag="VFR42_12735"
     CDS             3009..>3628
                     /locus_tag="VFR42_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU0370075.1"
                     /translation="MTPREFANSICTTLQNAGYQAFLVGGCVRDLLLKREPADYDVAT
                     NATPDQVLELFPNGITVGAQFGVILVPQDGMKVEVATFRSDIGYTDGRRPDRVQYSTT
                     PEEDVKRRDFTINGLLMRHDTDEALDFVNGQADLKAGIIRAIGEPEKRFAEDKLRMLR
                     AVRFAARFGYEIEAETFRAIRRHAKEIQQVSAERIREELTKMLTEGA"
BASE COUNT          746 a         1048 c         1109 g          725 t
ORIGIN      
        1 aatgatgcga gactctgaat ccgcagccgg aggacagccg tcatgaccct tttttccagc
       61 tggttctggc cagacgttag cactcgcaag aacgcgacgt tcgcgaccac cgaagccttt
      121 tacgtattgc tgggggtagc gatcctagtg gcactgttta ccttggtcga tttcgcgcgc
      181 gggtgggaat tcctcgatcg aattgcgcag gtggcagtga ctggccatcc gggcgctctt
      241 ttgctcgcaa ttgcggttgc gctcgctctc ttgcaccggg tgcggggcgc gtttgctttt
      301 cacaaatttg cagcgattcc ggcccatacg ccgagcatcg agcaaagctt tcgatccttt
      361 gggcagaact cgccgcggag cgagaaggac accgggcgga attaggccga tgccatgccg
      421 aagctggcgc aatttcgggc gaaacatgac gacgcgtcgg ccctgacgct gcccgccgga
      481 tccacgattc tgcatgtgga tatggacgca ttcttcgttt cggtggaatt gctggagcgg
      541 ccggagctac gcggcaagcc ggtagtcgta ggaggaaggc cggatcaacg cggtgtagtt
      601 tccgccgcaa gctacgaagc gcggaaattc ggaattcaat cggcgatgcc gctgcgcacc
      661 gcggcaagac tttgtccgca cgcaattttc ctggacggcc atcacgacaa atacggcgag
      721 tttagcgacc gcgtcgccac catcctcgcc cagttctcgc caatcgtgga aatggtttct
      781 attgacgaag cctaccttga tttggccgga accgagcgac tgcacggccc gccgtttgcc
      841 gcggcacacc aactgctgcg tatgattacc agtgaaacgt cgctgccctg ctccgccggg
      901 ctcgcgtcca cgcgcctggt tgcaaaagta gccagcgatc aggcgaagcc aaagggtttg
      961 ctgtggattc cggctgggca ggagtcgcga tttctggccc cgctgccggt gcgcaagatt
     1021 cccggcatcg gtgaagtcac cgaacgcgcg ttgcgcgcgc ttggcattga gtatgtgcac
     1081 caactcgccg atgcgggcct cgacaaactg gaaaagattt ttggccagtg gggcacggcg
     1141 ctgtaccgaa aggcgtgcgg tggcgattcc tacgaatttg ttctcgacgc cgagcccaaa
     1201 tccatctcgc acaatcacac attcggcgaa gacacggatg acaccccggt tctcgagtcc
     1261 atgctgagtc atctttcgca gaaagcctgc aagcgcctgc gcgaagcggg actctccgcg
     1321 cgcacgctga cactgaccat tcgctacgcc gggttcgata cgtacacgcg agccaaaacg
     1381 ccgccggatc cggtacagtt tgatgccgac gctttcgccc aatttctggc gctatttcgt
     1441 gagcagcgga acacgaagcg taagattcgc ctattgggcg tggcgttcag cgggttgata
     1501 cacggaaacg cgcaattgga cctgctggac ccgcaacgcc gcgccaagct tgaaaaactc
     1561 agcagagcgg cggattcgtt gcgtgaccgc tttggcttcg cgaaagtgca gttcggcggt
     1621 tcgctattgg cggaaaggcc agagcgcgaa aacaaaaagg gagagattcc gcgttttccg
     1681 cgcaacaagg agtaggacaa agctcgagcg gccttaagaa cactctaaaa agaccgcgcc
     1741 tgctctatac ttccgtttat gccttccacc gcgccgcctc ccgcgccgcc tcctgagtac
     1801 acgcgccagc cgcgccgcgt tcccggcgac ggcatatggg accgcatcag tgaaggccgc
     1861 cgtctggacg aactgtggtc gcaattcagc gccgacgcgc aagccagtta cggcttctac
     1921 ggcaaggatc tggattggga acagctcaag accattcccg cctggcgccg gccattccgc
     1981 gtagccaaac attttttctg ggcgctggtg atgaagatga cgccggcgcg gcgcgtgctg
     2041 ctgtttgtcg cgctggccat gctgattctt tccagcccga aattgaatgt gggccacagt
     2101 ttttcgttcg atgtgaacct ggatgcgatt gccgcgctgc tgtttctgtt gctgctttcc
     2161 ctcgaactgg cggacaaagt caccatgaag cgcgatctgg aaatcgcgcg ggaaattcag
     2221 agctggctga ccccttcgca gccgccgccg gtgaccggag ccagcatcgc gttccatacg
     2281 cggccgcaaa attccgtggc cggcgattat tacgacgcat tctatccgct gcaagatgca
     2341 gagtccggcg ggaagttatt tttagtgatc gccgacgtcg ctggaaagag tgtgccggcg
     2401 gcgctgctga tggcaacttt tcaagccagt ttgcacacgg tggcgcggga gggtgtttca
     2461 ctgcaaaagc tggtggcccg gctgaaccgc tatgcctgcg cacacagtct ggacgggcgg
     2521 agattcacta cggcgatgct gggggaatac gatccggcga cgcggatatt tacctacgtc
     2581 aatgccggac acaatgcgcc ggtgctccgg cgctacaacg ccaacggaga agcgcagatc
     2641 gaacggctgc cggccagcgg cgtgcctttc ggaatttccc tggacgcgaa ttacgaaatc
     2701 gcgaccgtgc agctggctcc gcgagacgca ttggtgcttt tcaccgacgg agtggtagag
     2761 gcgtttaacg cgaaaggtga agaattcaca gacgcgcgct gggtggacac ggtccgccat
     2821 ctacccgact gggaagcaca gcgcgctctg cagttcctga tgcagcgggt ggacgaattc
     2881 gtgggcgcca ccaagcagtt cgacgacatt acgtgtctcg tcgtacggtg cgaatagcgc
     2941 gttgcgcata ggtgcggccc cgccaatcgc ggcttctctc acgccctgcc caagagcata
     3001 atttatttgt gacaccgcga gaattcgcca attcgatatg cacgaccctg caaaacgccg
     3061 gataccaggc gtttttggtg ggcggctgtg tgcgcgacct cctactaaag cgcgagccgg
     3121 ccgactacga cgtagcaacc aatgcgacac ctgaccaagt gctggagctg ttccccaacg
     3181 gcatcacggt gggcgcgcag ttcggagtca tcctggtgcc acaggacggc atgaaggtgg
     3241 aagtggcgac attccgttcg gatatcggct acacagatgg gcgccgcccg gaccgcgtcc
     3301 agtactccac cacccccgaa gaggacgtga agcggcggga ttttacaatc aatggcttgc
     3361 tgatgcggca cgacacggat gaagcgttgg attttgtgaa cgggcaagcc gatctaaagg
     3421 ccggcattat tcgagccatt ggagagccgg agaagcgttt tgccgaagac aaattgcgga
     3481 tgctgcgcgc ggtgcgattt gcggcgcgct ttgggtatga gattgaagcg gaaactttcc
     3541 gcgccatccg gcgtcatgcg aaagagattc agcaagtttc ggcggagagg attcgcgaag
     3601 agctaacaaa gatgctgacg gaaggagc
//
LOCUS       DASTPM010000719         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1122079, whole genome shotgun sequence.
ACCESSION   DASTPM010000719 DASTPM010000000
VERSION     DASTPM010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122079"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..427
                     /locus_tag="VFR42_12740"
     CDS             185..427
                     /locus_tag="VFR42_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370076.1"
                     /translation="MSPQEIAAAQAQLNESTAVAQKNLDAARGRSLNPTQADLTSKVS
                     AFLQESKQAVREGDWTRAKNLAKKAEVLSQELAASL"
     gene            491..1633
                     /gene="thiO"
                     /locus_tag="VFR42_12745"
     CDS             491..1633
                     /gene="thiO"
                     /locus_tag="VFR42_12745"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEU0370077.1"
                     /translation="MKTFDAVIAGGGLIGAAIALELTRVGLRVGVFDAREPGREASWA
                     SAGILSPAPENSAMIAMVPLGRISLTMYPEFNAAVEELSGLPTGYRAKGTLEALFSRH
                     AKQELNTIIALHHGLGLEAEALSAQEAREVEPALSDEVEAAVLRPDEASVDNRLLAAA
                     LLAAAKRGGAQIFPGSPVQSIWREGSRCRGFILAAENKIVAGHTIIAAGCFSSQIAGV
                     EEFAPVRPAKGQMVSLRCPAAKIERVLWSERVYLVPRNDGRILAGATVEHVGFDKTVT
                     ASGIHANQGAAIELAPALAAAQVEETWAGLRPDTPDHLPIIGPADLGGLILATGHFRS
                     GVLLTPITARLVGDFVLGKTPSIDVERFSPMRFRAASASKTTGVHS"
     gene            complement(1641..2432)
                     /locus_tag="VFR42_12750"
     CDS             complement(1641..2432)
                     /locus_tag="VFR42_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370078.1"
                     /translation="MQFGETFARKAILCVAASAAIGVTASEPVRAAQQPDGPQMDAPA
                     PIAPTPAPARAPGQKLVVPPGTRLAVVLENGISTRSAKAGDSLYFHTAFPVTHNNQVV
                     IPVGSYLRGSLLESKRPGRIKGHGEFRMRLESLIFPNGYTVDLLAAPRSADTGGKETT
                     DSEGKVTGEGGKGKDVGTVATTTVTGAGIGAIAGGGKGAGIGAGVGGLIGLGAVLLTR
                     GPEAQLPRGTTLDIVLERELALDSNYVQFTNTGHAQPITAPPQRE"
     gene            2602..2802
                     /locus_tag="VFR42_12755"
     CDS             2602..2802
                     /locus_tag="VFR42_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370079.1"
                     /translation="MAKLALVYGMRLLPLDDLGEVHEATVVLKNGKSARITMHLIEGS
                     KEEIESTFHQSIEAFFEMYPEI"
BASE COUNT          536 a          923 c          818 g          588 t
ORIGIN      
        1 tcccggtgtc tcggtgaacc cggactcgct tgcagccgct gctcctgaca tttccctcga
       61 actcccctcc cttccctcgc ctctgggcct gaactagcag ccggcgcgtc cccgtgtccc
      121 agcccagttg gcgcctgaag ctgccgagtc acgcaagccg cctgctcctt ccctggagcc
      181 agagttgtct ccacaggaaa tcgcggctgc gcaggcccag ctgaacgaaa gcaccgccgt
      241 cgcccaaaaa aatctggacg ccgcaagagg acgttccctg aatccaactc aagccgatct
      301 cacttccaaa gttagtgctt tcttgcagga atcgaagcag gctgtccgcg aaggcgattg
      361 gacgcgcgct aaaaatctcg cgaagaaggc cgaggtgctt tcccaggagt tagcagcttc
      421 tctctgagaa aatcacgttc aggttttccg aaagcgagga acgccgcctg ggttgcgcta
      481 aactacgcag atgaaaacgt ttgacgcggt catcgccggt ggcggtctaa ttggcgccgc
      541 gatcgcactc gagttgaccc gcgtcggcct gcgcgtcgga gtgtttgacg cacgcgagcc
      601 cggccgcgaa gcatcttggg ccagtgcagg cattctttct cccgctcccg aaaattccgc
      661 catgatcgcg atggtgccgc tcggccgcat cagcctcact atgtatcccg agttcaatgc
      721 cgcagtggaa gaactcagcg gcttgccgac cggctatcgt gcgaagggca ctctggaggc
      781 gctgttctcg cgccatgcca agcaagaatt gaacacgatt atcgcgctgc atcacggact
      841 cggtctggaa gccgaagctc tttccgcgca ggaagcccgc gaagtggaac cggctctgag
      901 cgatgaagtc gaagcagccg tgcttcgtcc cgacgaagct tcagtggaca atcgcttgct
      961 cgctgcggcg ttgctcgcag ccgccaagcg cggcggcgct caaattttcc ccggctctcc
     1021 cgttcagtct atttggcggg aaggttcacg gtgccgtggc ttcattctcg ccgcagaaaa
     1081 taagatcgtc gcgggccaca cgatcatcgc cgcaggatgt ttttcttcac aaatcgccgg
     1141 agtggaggag ttcgctccgg tccgcccggc caagggccag atggtttctc tccgctgccc
     1201 agccgccaaa atcgagcggg ttctctggtc cgagcgcgtt tatctcgtgc cgcgcaacga
     1261 cggcagaatt ctggccggcg ccacggtgga gcacgtcggc tttgacaaga cggtcaccgc
     1321 gagcggcatt cacgccaacc agggggccgc aattgaactc gctcccgctc tcgccgccgc
     1381 gcaggtcgaa gaaacctggg ctggccttcg cccggacacg ccagatcatt tgccgattat
     1441 cggtcccgcg gatttgggcg gactgattct agccacggga cattttcgca gcggcgtcct
     1501 gctcacgccc atcaccgcca gactggtcgg cgacttcgtc ttgggaaaga cgccaagcat
     1561 agacgtggaa cgattcagcc ccatgcgctt ccgcgcagcg tcagcttcga aaacgactgg
     1621 cgtccacagt tgattcttta ttattctctc tgcggcgggg ccgtgatcgg ctgcgcgtgg
     1681 ccggtgtttg taaattgcac gtagttcgaa tcgagcgcca gctcgcgctc caggacaata
     1741 tccaacgtgg tgccgcgcgg cagctgcgcc tccggcccgc gcgtcagcaa taccgctccc
     1801 aatccaatta aaccgcccac gcccgcgcca atgccggctc ccttgccgcc gccggcgatc
     1861 gcgccaatcc ctgctccggt cacagtcgtc gtcgccacgg ttccgacgtc tttgcccttg
     1921 ccgccctcgc cggtaacttt tccttccgag tcggtggtct ctttcccgcc cgtatccgcg
     1981 ctgcgcggag cggccagcaa atctaccgtg tagccgttcg ggaagatcag cgattccagg
     2041 cgcatgcgaa attcgccgtg acctttgatc cggcccgggc gcttgctctc cagcaacgat
     2101 ccgcgcaagt acgatcccac gggaattacg acctgattgt tatgggtgac gggaaacgcg
     2161 gtgtgaaagt acagcgaatc gccggccttg gcgctgcggg ttgaaattcc attttccaac
     2221 actacggcta ggcgcgtgcc cggtggtacg acgagtttct gccccggtgc acgtgccggc
     2281 gctggtgtcg gagcaatcgg cgcgggcgca tccatctgcg gtccgtcggg ctgttgcgct
     2341 gcacgtaccg gttcagatgc agttacgcca atcgctgcgc tggccgcgac gcacaggatc
     2401 gctttccgcg cgaaagtttc gccaaattgc atggggcttc ctccgttatg gttcttccga
     2461 tgcggagttc cagtggacgg atgccccagc atatcgccgg acacgatgcg agcacaatgc
     2521 gtcgtcatcc gcgcgacccg atttgacact gcagcccctc ccggtcagtc tgaaaggcta
     2581 ccaatcctag gagaattcgc aatggccaag ctggctttag tttacggaat gagattgctg
     2641 cccctggacg accttggtga agtccatgag gcaaccgtcg tcctcaaaaa cggcaagtcc
     2701 gcgcgtatca ccatgcacct gatcgaaggc agcaaggaag aaatcgagtc tacctttcac
     2761 caatcgattg aagctttttt tgagatgtat ccagagatct agctggcgcc catccgccgt
     2821 cgctgcgcgc agcgaatttt gcgttagcga ttcccgaaat tttcc
//
LOCUS       DASTPM010000720         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_141496, whole genome shotgun sequence.
ACCESSION   DASTPM010000720 DASTPM010000000
VERSION     DASTPM010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141496"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..2264
                     /locus_tag="VFR42_12760"
     CDS             273..2264
                     /locus_tag="VFR42_12760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEU0370080.1"
                     /translation="MGPEICANLAAAEQREWLVTNGIGGFASGTIAGSSTRRYHGLLF
                     AALNPPAARTLLVGGVDEVIRIGEQAFELGAHRWLTGAVAPEGYRSLRGFRLEGTVPV
                     WTYQAGPAQLEKSIWMPHGENATYVGYRIAESSSPIELELKVLVNYRDFHGSTHAGSP
                     PSDWRMLVEPAEHGARVVAYEGAAPFYVRSRAASCELQHVWYRDFFYPLERERGLDDH
                     EDQLFAARFRAQLKPKQSITFVFSTSETASLDGESVLAEERVRQSKVLAAATSLLRPP
                     PASDSPPSWLAPLVLAADQFLVRRSLPNQPDGKTVVAGYHWFGDWGRDTMIALPGLAL
                     VTGRAEIAKEILLAFASFVDRGMLPNNFPDAGGTPEYNTIDATLWYFEAIRQYFAATE
                     DVATLNKLFPILAEIIEAHVHGTRYNIHLDVDGLLYGGGPGAQLTWMDAKVGDWVVTP
                     RTGKPVEVNALWINALESMATFATRLGKAAGVYTQHASAAKSSFGKFWNVERNCCFDV
                     IGAPGVGNDATLRPNQIFAVSLVGNLLSAEQQKAVVDVCAEQLLTAVGLRSLAPGESG
                     YVGRYAGGVRERDAAYHQGTVWGWLLGPFALAHYRVYRERAAALAFLEPLAKSIDRYS
                     IGTLGEIFDGEAPHPARGCISQAWTVGEILRVWHALSRP"
     gene            complement(2332..>2789)
                     /locus_tag="VFR42_12765"
     CDS             complement(2332..>2789)
                     /locus_tag="VFR42_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0370081.1"
                     /translation="TDINAGITVYYSSGPIAPALRASCAYPGLFVPIQYDGRTLVDGF
                     LTSPVPVEGALLLGAEVVIAVFLESGGIENPRTAVDIISRSFNIIQGHSEIAWRQQAD
                     AVIEPDVTSFAWDDFTKTPELIAAGEAAAKKAIPDILALLRRQAKDSRT"
BASE COUNT          556 a          776 c          875 g          582 t
ORIGIN      
        1 ctgtggcggg ggcactggag cattttcaat ccgatccgtg ctggcgcgac ttgattctgt
       61 tccacgaata ttttcacggc gacaacggcg cgggtattgg cgcaaatcac cagactggct
      121 ggacgggact ggtcgccaaa cttctggaac aaagcggtga atgagttgtt gtgatggccg
      181 ctccgagaaa accacgcgcg tcgtcgcgaa cgaaaaaaac gagctcgaag aaacagccgc
      241 agccggaacc gattgcagac tcagccattt ctttggggcc tgaaatttgt gcgaatcttg
      301 ccgcggccga acaacgcgaa tggctggtga ccaacggcat cggagggttc gcttcaggga
      361 ccatcgccgg atcgtctact cggcgctatc atggcctgct ttttgccgcg ctgaatccgc
      421 ccgcggctcg tacgttgctt gtcggcggcg ttgacgaagt gatccgaatt ggcgagcaag
      481 ctttcgagct cggtgcgcat cgctggctga ccggggccgt cgcgcccgag ggctaccgca
      541 gcttgcgtgg ttttcgactc gagggaacgg ttcccgtttg gacgtaccaa gctggtccgg
      601 cgcaactcga aaaaagcatc tggatgccgc acggcgaaaa cgccacttat gtcggctaca
      661 ggatcgcgga atcctcgtcg cccatcgaac tcgagctcaa agtcctcgtc aactatcgtg
      721 actttcacgg atcaacgcat gctggctcgc ctccgagcga ctggcgcatg ttggttgaac
      781 cggcggagca cggagctcga gtggtagcgt acgagggtgc agcgccgttc tatgtacggt
      841 cacgtgctgc aagctgcgaa cttcagcacg tctggtatcg agacttcttc tatccactgg
      901 aacgcgagcg cggcctggac gatcacgagg accagctttt cgccgcgcgg tttcgcgccc
      961 agttaaaacc taagcagagt attactttcg ttttctcaac tagcgagacc gcgtcgttgg
     1021 acggagagag cgtccttgcg gaggagcgcg tgcgccaaag caaggtactt gctgcggcca
     1081 catcgctttt gcggccgccg cccgcaagtg attcgccgcc gtcctggctc gcgccacttg
     1141 tccttgccgc ggatcagttt ctagttcgcc gttcgctgcc gaatcaacct gacgggaaaa
     1201 cggtcgttgc gggttaccac tggttcggcg attggggccg cgacaccatg attgcgctgc
     1261 ccggcttggc gttggttacg ggccgcgcgg aaatcgccaa agaaattctg ttagcgtttg
     1321 caagcttcgt ggaccgtggc atgctcccga ataattttcc tgacgccggt ggaacgcctg
     1381 aatacaacac cattgatgcg acgctgtggt atttcgaggc cattcgccag tattttgcgg
     1441 ctacggaaga tgtggcgact ctgaataaac tgtttccgat tcttgccgaa atcatcgaag
     1501 cacatgtcca cggcacgcga tacaacattc accttgatgt ggatggattg ctctatggcg
     1561 gagggccggg agcgcagctt acctggatgg acgcgaaagt tggcgattgg gtggtgacgc
     1621 cgcgaaccgg caaaccggtc gaggtgaacg cgctttggat taatgcgctg gaatctatgg
     1681 ccaccttcgc gacgaggttg ggcaaggctg ctggcgttta cacacaacat gcgtccgcgg
     1741 ctaagtccag tttcggcaaa ttctggaatg tcgagcgcaa ctgctgcttc gacgtaatcg
     1801 gcgcgccggg ggtcggaaac gatgcaaccc tgcggccgaa tcagatattc gcagtttcgc
     1861 ttgttggcaa tctcctttct gcggaacagc aaaaagctgt tgtggacgtt tgtgcggaac
     1921 aacttctcac ggctgtgggt ttgcggagcc tagcgccggg cgagtcaggc tatgtggggc
     1981 gctacgccgg tggtgtgcgt gagcgcgacg ccgcctatca tcagggaacg gtttggggct
     2041 ggctgctcgg tccattcgcg ctcgcccatt atcgcgttta ccgggagcga gcggcagcgc
     2101 tggccttttt ggaaccgcta gcaaagagca tcgatcgcta cagcatcggc acgctcggcg
     2161 aaattttcga tggcgaggca ccacacccgg cgcgcggctg catttcgcag gcttggaccg
     2221 tcggcgagat cctgcgcgtt tggcacgcgc tgagccgccc gtagaacatg gccaggaaat
     2281 cgatcaccca gatcttgcaa ccgggtttac gagccgcgtg agaagagtgc gctaagtgcg
     2341 ggaatctttc gcctgccggc gcaacagcgc aagaatgtcc ggaatggcct ttttcgcggc
     2401 agcctcgccg gcggcgatga gttcgggagt cttcgtaaag tcgtcccagg cgaacgaggt
     2461 gacgtcgggt tcgatcaccg catccgcttg ctggcgccag gcgatttcgg aatggccctg
     2521 aatgatgttg aaagagcggc tgatgatgtc gacggcagtg cgcggatttt cgatgccgcc
     2581 ggattcaagg aaaacggcga tgacgacctc cgccccgagt aacagcgccc cttccacagg
     2641 aaccggcgaa gtcaggaagc cgtccacaag tgtccgcccg tcatattgaa taggaacgaa
     2701 aagacctgga taggcgcaag aggcgcgcag cgccggagcg atcggaccac ttgagtagta
     2761 gacggtgatg ccggcattga tgtcggtgg
//
LOCUS       DASTPM010000721         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_83790, whole genome shotgun sequence.
ACCESSION   DASTPM010000721 DASTPM010000000
VERSION     DASTPM010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83790"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VFR42_12770"
     CDS             <1..1416
                     /locus_tag="VFR42_12770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0370082.1"
                     /translation="DFVPSYDGFLADLSPRDRERMEAAIKDSLEKKVGHSIEYQFALS
                     NGDLRTISCTWELSLDEAGTPVRIFGTCQDITDSRRAQAEAFARQKLESIGVLASGIA
                     HDFNNLLGAVLAQTELAIAELAAGSRPDEELKAIQEVAVRGAEIVRQLMIYGGKESDS
                     LELVDVSKVIEGMLGLLRIAVSRRTLLITNLGKDLPAVRARPAQLSQIVMNLVVNASD
                     AIGDRDGIVRVTTDHVPAHGIEPIANEAPGGAYVRLEVSDTGCGMSPETQTRAFDPFF
                     TTKVSGRGLGLSVVHGIVRSLAGNIQVASEPGKGAAFQVLLPCAGDAPPQCTEEAIPV
                     DNSTTRARRATVLVVEDEDQLRHAVAKMLRKSGLEAVEAADGSAAIDLLRSQGGEIDL
                     ILLDLTIPGRSSMEVLAQAATSRPDGIKVILASAYSEEVARPLMQAPIVCGFIRKPFG
                     LGELLQGIRTAISHIERAAGN"
     gene            complement(1681..>2406)
                     /locus_tag="VFR42_12775"
     CDS             complement(1681..>2406)
                     /locus_tag="VFR42_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0370083.1"
                     /translation="EFAKKIFQGRNPVGRVFRREEFGNTPDSEFLVVGVVRNTKYYEL
                     REDFKPIAFFPEAQVKEPGQGGNFVVRTNAPLGEFYRNAEAAVAEIHPQNGVEFLVLT
                     KRIQQTLMRDKLMALLAGAFGVLAGTLAVLGLYGVIAYMVARRRNEIGVRVALGASQH
                     SVIGLVMREAFLLLSAGLAIGLALAAWSEEAAASLVYGMKPRDPVTLAGAALLLAAVA
                     LIASFAPASRAARVQPMDALREE"
BASE COUNT          494 a          778 c          674 g          460 t
ORIGIN      
        1 gattttgtcc ccagctatga cggatttctc gccgatctca gtccgcgaga ccgcgaacgg
       61 atggaggcag cgattaaaga ctcgctcgag aaaaaagttg gacactccat cgaatatcag
      121 tttgcgcttt ccaatggcga tctgagaacc atctcctgca cctgggagct atcgctcgat
      181 gaggccggga cgcctgtgcg catattcgga acctgtcagg acattaccga ctctcgccgt
      241 gcccaggccg aagcgttcgc caggcagaag ttggaaagta taggggttct ggcaagcggc
      301 atagcgcacg atttcaataa cctgctgggc gcggttctgg ctcagacgga attggcgatc
      361 gcggaacttg ccgccggatc gcggcccgac gaagaactga aagcgataca agaggtcgca
      421 gtccggggcg cggagatcgt tcgccaacta atgatctatg gggggaaaga gagtgactcg
      481 ctggagttgg ttgacgtttc caaagtcatc gaaggaatgc tgggtctgct taggattgca
      541 gtctcccggc gcacccttct gatcaccaat ctcggcaagg atctcccggc cgttcgagca
      601 cgtcctgcgc aattgagcca gattgtgatg aacctcgttg tgaacgcctc ggatgccatt
      661 ggcgatcgcg acggtattgt ccgcgtgact acggaccatg ttcccgcgca cggtatcgaa
      721 ccgatcgcca acgaagcgcc cggcggggcc tacgtccgat tggaagtttc cgataccggc
      781 tgtgggatgt cgcccgagac gcaaactcgg gccttcgacc cgttcttcac caccaaggtt
      841 tccggccgcg gactcggact atcggtggtc cacggaattg ttcggagtct cgccggcaat
      901 atccaggttg cgagtgaacc gggcaaaggg gcggcgtttc aggttctgct tccctgcgcc
      961 ggcgatgcgc cgccacaatg taccgaagaa gcaattcccg tcgataattc cacgacccgt
     1021 gcgcgtcggg cgacggttct ggtagtggag gatgaggacc agttgcggca tgcagtcgcc
     1081 aagatgctcc gcaaatccgg cctggaggcc gtcgaagcag ccgatggttc tgcagctatc
     1141 gatcttcttc gctcccaagg tggcgaaatc gacttgatcc tgctggacct gaccattccg
     1201 ggacgttcaa gtatggaagt ccttgcccaa gccgcgacgt cgcgtcccga tggcatcaaa
     1261 gtcatccttg ccagcgccta tagcgaagaa gtcgccagac cgttgatgca ggccccgatc
     1321 gtctgcggct tcattcgcaa gccctttggg ctgggtgagc ttttacaggg catacgcacg
     1381 gcaatcagcc acatcgaacg ggccgccggt aattaaaggc tggcagctaa cctgccgaac
     1441 gacgccgcag cccaaaacgc cgcacgagaa aacgtgcatt tggacagaaa attgccggta
     1501 ctctgaatga ggatctccaa gggctcacgc tccaccgcaa tttccgcgcc taggccgtgc
     1561 caatgggctg cgtctcgcag tggttgtggg ccaagcgatc tatttcctat caggccgcaa
     1621 gcgcagccgc ctcacatcaa ccgtcccacg atggaggagc gtcctgtttc tcgctgaccg
     1681 ttactcttcc cgcaacgcat ccatcggctg cacccgcgcc gcgcgactag ccggcgcgaa
     1741 gctggcaatc agagccacag ccgcaagcag cagtgctgct ccggccaatg tcaccggatc
     1801 cctcggcttc atcccataca ccagcgatgc cgccgcctct tccgaccacg ccgccagcgc
     1861 cagcccgatc gccaggcccg ccgaaagcag caggaacgct tcccgcatca ccagcccgat
     1921 cacgctgtgt tggcttgcgc ccagcgcaac gcgcacgccg atctcgttcc gccgccgcgc
     1981 caccatgtac gcgatcacgc cgtagagccc caaaaccgcc agcgttcccg ccaacacgcc
     2041 gaaggctccc gccagcagcg ccatcagttt gtcgcgcatc agggtctgtt gaatccgctt
     2101 cgtcagcacc aggaattcga cgccgttttg cggatgaatc tccgccaccg ccgcctcggc
     2161 gttacgatag aactcgccca acggcgcatt cgtgcgcacc acgaaattcc ctccctgtcc
     2221 aggctcctta acctgcgcct ccgggaaaaa ggcaatcggc ttgaaatcct cgcgcagctc
     2281 ataatatttc gtgttgcgca cgacgccgac cacgagaaat tccgagtccg gagtgttccc
     2341 gaactcctcc cgccggaaca cgcgccccac cggattccgc ccttggaaga tcttcttcgc
     2401 gaactc
//
LOCUS       DASTPM010000722         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_805243, whole genome shotgun sequence.
ACCESSION   DASTPM010000722 DASTPM010000000
VERSION     DASTPM010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805243"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /gene="bioA"
                     /locus_tag="VFR42_12780"
     CDS             complement(<1..1072)
                     /gene="bioA"
                     /locus_tag="VFR42_12780"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEU0370084.1"
                     /translation="MDPAPIEIARAEGVWLHAKDGRKIIDAVSSWWVNLHGHANPRIA
                     AAIAEQARKMEHVILAGFTHEPAEELATRLRKWVAPELMHLFFSDDGSTAVEVALKLA
                     VQHFSNSGRPEKCEIVALKHGYHGDTAGAMSVSDDSPFTDPFRTMRYPVHRVHSAYCY
                     RCPVGLRRETCHIECVGKLQKLLEDRADRIAAVIVEPLLQAAGGMIVHPVEFLQKVRA
                     LSSKHGVLLIADEVLTGFARTGKMFACDLAGVTPDLMCLSKGITGGFLPMGATLCSER
                     VAGAFHSENRMHTFYHGHSYTGNALACAAANASLRIFDDEPVFDRIARIATIHRERLE
                     QLRKYAAVGDTRQIGTIGAIELR"
     gene            complement(1129..1764)
                     /gene="bioD"
                     /locus_tag="VFR42_12785"
     CDS             complement(1129..1764)
                     /gene="bioD"
                     /locus_tag="VFR42_12785"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEU0370085.1"
                     /translation="MRHHFFVTGTDTGVGKTTLSALLCAALDAVYWKPIQTGTLEGSD
                     RATVMRLAGIGTDRTIDEVYKFVPPVSPDLAARWAGVEIDLAKINLPNHLANDWLIVE
                     GAGGVLVPINQKQFILDMMVKLKLPVILAARSTLGTINHTLLTLAALHAAELPVHGVV
                     LIGDQNRENRETVERFGHVRVIGEVPRLPNLHRTSLMQVFMNHFDRSAFSQ"
     gene            complement(1761..2951)
                     /locus_tag="VFR42_12790"
     CDS             complement(1761..2951)
                     /locus_tag="VFR42_12790"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEU0370086.1"
                     /translation="MPSEAQSKLVRELERDLAELCARGQLRSFAQIAGVNLCSNDYLG
                     LAQDARLKESVLEAVAGSERVGGTGSRLLSGHDPVWDELEEEFAALARTETALYFANG
                     YAANLGLLSSLLGKGDLVFSDALNHASLIDGIRLSGARKEIYAHVDLNALERALRLHE
                     SERCRKLIVTESVFSMDGDIADVNGLQKLAERYGASLLVDEAHATAVQGPGGAGIVAK
                     ARLTRDVFATVHTCGKALASAGAFVCGSRVLREFLINHARTLIFSTAMPPYLAWQIRA
                     ALRLARGMDAEREALHANSAELAQLLRGDGWETSESASQIVPVIIGSNQEALSAAGFL
                     QEQGFAVRAIRPPTVPAGTARLRLSLTARVSREQVTGIRAALQNWRRSQSSLAAAEVA
                     TGRG"
     gene            complement(2941..>3294)
                     /locus_tag="VFR42_12795"
     CDS             complement(2941..>3294)
                     /locus_tag="VFR42_12795"
                     /inference="COORDINATES: protein motif:HMM:NF018651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370087.1"
                     /translation="RIGLLQQLACLKPHPESVPINMLVRVEGTPLAKVPALAPLEMVR
                     AIATARILMPASRVRLAAGRKQLSPEAVTLCFLAGANSIFVGEKLLTTPNPGRDEDQE
                     LLATLGMKPLEPHAV"
BASE COUNT          685 a         1151 c          824 g          634 t
ORIGIN      
        1 tgcgcagctc gattgctccg atcgtcccaa tctgccgcgt atcgcccact gcagcatact
       61 ttcgcaattg ttcgaggcgc tctctgtgaa tcgttgcgat tctggcgatg cgatcgaaaa
      121 ccggttcatc atcaaaaatc cgcaagctgg cattcgctgc ggcgcacgcc agtgcattcc
      181 cggtatacga atgaccgtgg tagaaggtgt gcatccggtt ttcgctgtga aacgcccccg
      241 ccacgcgttc gctgcacagc gtcgcgccca tcggcaaaaa tccgccggta atccctttcg
      301 acaggcacat caggtccggc gtaactcccg ccagatcgca tgcaaacatt tttcccgtgc
      361 gcgcaaaacc cgtcagcact tcatcggcaa tcagcagcac cccgtgcttg ctagaaagtg
      421 cccgcacctt ttgcaagaac tctaccggat gcacaatcat tccgcccgcc gcttgcagca
      481 acggttcgac aatcaccgcc gcgatgcggt cggcgcgatc ctcgagcagc ttttgtaatt
      541 tcccaacgca ctcgatgtgg cacgtttcgc gccgtagtcc caccgggcaa cggtagcaat
      601 acgccgagtg cactcgatgc acgggatacc gcatcgtccg aaacggatcg gtgaatggcg
      661 aatcgtcgct gaccgacatc gcccccgccg tgtccccgtg atagccatgc ttcagcgcca
      721 caatctcgca tttttccggg cggccgctgt tgctgaaatg ttgcacggcc agcttcagcg
      781 cgacttcaac ggcggtcgag ccgtcatccg aaaaaaagag atgcatcagc tccggagcga
      841 cccatttgcg caagcgcgtc gcaagttcct cggcgggctc atgggtgaac ccggccagaa
      901 tcacgtgctc cattttgcgc gcctgttccg caatcgccgc cgcgattctc gggttcgcat
      961 gcccgtgcag gttcacccac caggacgaca ccgcatcgat aatctttcgc ccgtctttgg
     1021 cgtgcagcca cacgccctcc gcgcgcgcaa tttcaatcgg cgccggatcc aaggcggaat
     1081 ttgtaaacgg atgccagatg cggagaggag aagacattct atggaaggtt attgggaaaa
     1141 cgcggaacgg tcaaaatgat tcatgaacac ctgcatcagc gaagtgcgat gcaaattcgg
     1201 caaacgcggc acttcgccga tgacccgcac gtgaccaaag cgctccaccg tttcgcggtt
     1261 ttcgcggttc tgatcgccga tcagcaccac gccatgcact ggcagctccg ccgcatgcag
     1321 cgccgccagc gtcagcaaag tgtgattaat ggtgcccagc gtgctgcgcg ccgccagaat
     1381 taccggcagc ttcagcttca ccatcatgtc cagaataaat tgcttttggt tgatgggcac
     1441 caacactccg ccagcgcctt ccacaatcaa ccaatcattc gccaggtggt tcggcaaatt
     1501 gattttcgcc agatctattt ccacgcccgc ccagcgcgcc gccaaatccg gcgaaaccgg
     1561 cggaacaaat ttgtaaacct catcgatggt acgatccgta ccgattcccg ccagccgcat
     1621 caccgttgcg cggtccgagc cttccagcgt gcccgtctga atcggtttcc agtacaccgc
     1681 gtccagtgcg gcacacaaga gcgcggaaag cgtggtcttg cccacacccg tgtccgtgcc
     1741 agttacaaaa aagtgatgcc tcatccgcgg cccgtcgcaa cttccgccgc agccagcgag
     1801 ctctgcgacc ttctccaatt ctgcaaggcc gcccgtatgc ccgtcacctg ctctcgcgaa
     1861 acccgcgccg taagcgacaa tctcagccgc gccgtccccg ccggtaccgt cggcggccga
     1921 atggcccgca cggcaaatcc ctgctcctgc aagaatcctg cggcggaaag cgcttcttgg
     1981 ttcgatccga taatcaccgg cacaatctgc gaagccgatt cgctggtctc ccatccatcg
     2041 ccgcgcaaca actgagccag ctctgccgag tttgcgtgca gcgcttctct ctccgcatcc
     2101 attccccgtg ccaaccgcaa cgctgcgcga atctgccacg ccagataagg gggcatcgcc
     2161 gtactaaaga tcagcgtccg agcgtgattg atcagaaatt ctcgcagcac gcgcgagccg
     2221 caaacgaatg ctcccgcgct cgccagcgct ttcccgcaag tgtgtaccgt cgcaaaaaca
     2281 tcacgcgtca gccgcgcctt cgcgacaatg cctgccccgc ccggtccctg cactgccgtc
     2341 gcatgcgctt cgtccaccag caaactcgcg ccatagcgtt ccgccaactt ctgcaacccg
     2401 ttcacgtcgg cgatgtcgcc gtccatgctg aacaccgact ccgtcacaat cagcttccgg
     2461 caccgttcac tttcatgcag ccgcagagct cgctccagcg cattcaaatc cacatgcgca
     2521 tagatttctt tccgcgcgcc tgaaagccga atcccatcaa tcaagctggc atgattcagc
     2581 gcgtcggaaa agaccaaatc gcctttccca agcagggaac tcagcaagcc aaggttcgcc
     2641 gcataaccat tcgcaaaata gagcgccgtc tcggtgcgcg caagtgccgc aaactcttcc
     2701 tccaactcat cccacaccgg atcgtgcccg gacagcagcc gcgaacctgt tccgcccacg
     2761 cgctccgatc cggccactgc ttccagcacc gattccttca gccgcgcatc ctgcgccaat
     2821 cccaaataat cattggaaca aagattcacg cccgcaatct gcgcaaagct gcgcaactgg
     2881 ccccgcgcgc acaactctgc cagatcgcgt tccagctccc tcaccagttt gctttgtgct
     2941 tcagacggca tgcggctcca gcggcttcat gcccagcgtg gccaacagct cctggtcttc
     3001 atcgcgtccg ggattcggcg tggtcaataa tttctcgccc acaaaaatcg aattcgctcc
     3061 cgccaggaag cacagcgtca ccgcctcggg cgaaagctgt ttgcgtcccg ccgccaagcg
     3121 cactcgcgaa gccggcatca gaatccgcgc cgtagcaatg gcacgcacca tttccagcgg
     3181 agcgagcgcg ggcaccttcg ccaacggcgt cccttctact cgcaccagca tgttgatcgg
     3241 cacgctttcc ggatgcggct tcagacacgc caactgctgt agcagcccga tgcg
//
LOCUS       DASTPM010000723         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_516459, whole genome shotgun sequence.
ACCESSION   DASTPM010000723 DASTPM010000000
VERSION     DASTPM010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516459"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VFR42_12800"
     CDS             <1..1084
                     /locus_tag="VFR42_12800"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU0370088.1"
                     /translation="LYMRHDSLGHLEHNPTFYIVFALVALAAATLMNLVGLDVGTWLH
                     NLGALAMWIPALIIILMGAIAFHRFGSATVFNVHTMIPSTQIKDMVFWSVLIYAITGC
                     ETASLMGDEIKNARRAIPWALFVAGVTVAFCYIVGTIGVLLALPAAEVGNLQGLMQAI
                     SSTAQKIHWEGVIPFAAFLIMMSNVGAAGAYLAAGARLPYVAGIDRFLPPAFGALHPR
                     WKTPWIGLLTQFLFGAIFIFLGQAGTSVKGAYDVLVSMGVITYFIPFLYLFAAMFKLQ
                     SQPAGLDVIRVPGGKPVARLVAIVGLLTTTATIAISVLPPPDEPNKPLAVLKIVGLSG
                     LMVLMGVAIFYRPRIRRALGGWRRAS"
     gene            complement(1119..2162)
                     /locus_tag="VFR42_12805"
     CDS             complement(1119..2162)
                     /locus_tag="VFR42_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU0370089.1"
                     /translation="MLGWTESQSQDLSNAAMASPTGRVFVLKHDIVGTSAKVRELLEL
                     IQRVAPTSSTVLIQGESGTGKELVARALHRNSPRAAGPLVAINCAALTESLLESELFG
                     HEKGAFTGAVARKKGKLEMAEGGTLFLDEISELAPGMQAKLLRALQEREFETVGGTRP
                     VKVNVRVIAATNRDLQEAVLAGRFRNDLFYRLNVVSISTPPLRERREDIPALAESFIV
                     KVSGKYRVPMKTLSQEVVTQLMRYEWPGNVRELENAIERAMVLGATEELRPEDLPPAL
                     FESAMADQHGEVGFQGAVKENKKQLVLQALEKAGGYYVDAAKILGLHPNSLLRLIRTL
                     GLRSVNGQGSASC"
BASE COUNT          440 a          801 c          601 g          551 t
ORIGIN      
        1 tctctacatg cgccacgatt ccctcggtca cctcgagcac aatcccacgt tctacatagt
       61 attcgccttg gtcgctctcg cggccgcgac tttgatgaac ctcgtcggcc tcgatgtcgg
      121 cacctggctg cacaatctcg gcgcgctagc catgtggatt cccgcactca tcatcatcct
      181 gatgggcgcc atcgccttcc accgttttgg gtcagccacc gtcttcaacg tccacaccat
      241 gatccccagc acccagatca aagacatggt tttctggtcc gtcttgatct atgcaatcac
      301 cggatgcgaa accgcgtcgc tcatgggaga tgaaatcaag aacgcccgcc gcgcgattcc
      361 ttgggccttg tttgtcgcgg gcgtgaccgt cgccttctgc tacatcgtgg gcacgattgg
      421 cgtattgctc gcgctgcctg ccgctgaagt cggcaatctc cagggcctga tgcaagcgat
      481 ttcgagcaca gcgcagaaga ttcactggga aggcgtgatc cccttcgccg cttttctcat
      541 tatgatgagc aacgtcggag ccgccggagc ctatctcgcc gctggagcac gccttcccta
      601 cgtggccggc atcgatcgct ttctcccgcc cgccttcggc gcgctgcatc cgcgctggaa
      661 gactccctgg attggactgc tcacgcaatt cctcttcggt gcaatcttca tttttctggg
      721 gcaggccggg accagcgtca aaggcgctta tgacgttctg gtcagcatgg gcgtaatcac
      781 gtatttcatt cctttccttt acttgttcgc cgcaatgttc aagttgcaat cgcagcccgc
      841 aggtctagat gtgattcgcg tgcccggagg caagccggtt gcgcgcctgg tcgcgatcgt
      901 cggacttctc actacaaccg cgaccatcgc catttcggtt cttccgccac ctgatgaacc
      961 gaacaagccc ctcgccgttc tcaagattgt tggattgtcg gggctgatgg tattgatggg
     1021 cgtcgcgatc ttctaccgac caagaattcg ccgcgcgctc ggtggctggc gacgcgcatc
     1081 ttagaaagag ttttcttgcc agcagcccgt agccgaattc agcacgacgc gcttccttgc
     1141 ccattcaccg accgcaaccc cagcgtccgg atcagccgca acaaggaatt cggatgcagt
     1201 cccagaatct tcgccgcgtc cacatagtaa ccaccggcct tctcgagcgc ctgcaggacc
     1261 agctgcttct tgttctcctt cactgcaccc tgaaaaccaa cctcaccgtg ctgatcagcc
     1321 attgccgact cgaacaaagc tggcggtaag tcctccggac gcaattcctc ggtcgctccc
     1381 aacaccattg cacgctcgat cgcgttttcc agttctcgca cgttccctgg ccactcgtaa
     1441 cgcatcaatt gcgtcaccac ctcttgcgat aacgtcttca tcggtacccg gtatttcccg
     1501 ctgaccttca cgatgaaact ctccgccaga gcgggaatgt cttcgcgccg ttcgcgcagg
     1561 ggtggagttg aaatgctgac cacgttcaac cggtagaaga ggtcattgcg gaaacgtcct
     1621 gccaggaccg cttcctgcag gtcgcgattg gttgcagcaa tcacccgcac attgaccttg
     1681 acggggcgcg tcccgcccac cgtctcgaat tcgcgctcct gcagagcgcg cagtagcttg
     1741 gcctgcattc cgggagcgag ttcgctgatc tcatccagaa acagagttcc gccctccgcc
     1801 atctcgagct tgcccttctt gcgcgcaacc gcccccgtga acgcaccctt ttcatgaccg
     1861 aagagttcgc tctcgaggag ggattccgtc agggctgcgc aattgatggc caccaaaggg
     1921 cccgcagccc gcggactgtt tcggtgcaga gcacgcgcca ccagttcttt tcccgtcccg
     1981 ctttcgcctt ggatcaacac cgtcgacgaa gtcggcgcga ctcgctgtat cagttccaac
     2041 aactcgcgca ctttcgcgct cgtcccgacg atgtcgtgct ttaacacgaa gacacggccg
     2101 gtgggcgatg ccatcgccgc attcgacaaa tcctgtgatt gtgattctgt ccaaccaagc
     2161 actctcgttc tccctcaatc gccatccctg ctatccgaac ggcgagtgcc ttctaactgc
     2221 tcagttagtg aatcggtgcg tagaatcgtt acaagatttg ttcgcacccg aacgcgcgag
     2281 cctccgacct gcattgtcct caacagtttg ccccggcgca tcccttcccc ggacctggcg
     2341 caaaccgctt ggcatgcgac gtgcttttac tctgcgcata cggatttcaa acc
//
LOCUS       DASTPM010000724         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1237480, whole genome shotgun sequence.
ACCESSION   DASTPM010000724 DASTPM010000000
VERSION     DASTPM010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237480"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /gene="hemN"
                     /locus_tag="VFR42_12810"
     CDS             <1..1467
                     /gene="hemN"
                     /locus_tag="VFR42_12810"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU0370090.1"
                     /translation="PTRHGVQWPARIESESTAVTIQQPSFEQAKRELRVTEEFLAHYN
                     RPGPRYTSYPTAPVWNDAFGPGDLEEVHSAADAARSPVSLYMHLPFCESLCLFCACNV
                     IIQKDKTVALPYFDVLKKEISRVAQAVSRHRDVVQFHWGGGTPTYSSPAQLEDLFAYT
                     KERFTFSPHAEIGIEVDPRVTSREHLETLRRLGFNRLSMGIQDFHEPVQKAIHRFQPY
                     EITRDLIQSARALGFDSINVDLIYGLPHQTPDTFAHTVDQIIGLAPDRIALFSYAHVP
                     WLKKQQGAFAMFLPEGMQKFEIFRTGLLKFLEAGYLYIGMDHFARPNDELATAQTNRT
                     LHRNFQGYTTKAGADLYGMGVTAISGFSDAYAQNFRELPAWEKAVNERGIATMRGYHL
                     SQEDRLRREVISRLLCHTVIPKREINQTFGIDFDSHFAEELLRLELPREDGLLTIDRE
                     EIRTTWLGRIFIRNLAMLFDPYLEKQHLDAKPLFSKTL"
     gene            1471..2853
                     /gene="hemG"
                     /locus_tag="VFR42_12815"
     CDS             1471..2853
                     /gene="hemG"
                     /locus_tag="VFR42_12815"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU0370091.1"
                     /translation="MACAPLTVVIGAGISGLACAYALQKKGQDVLVVEAASRPGGVIQ
                     SVEKDAYLLEQGPQSFSGTPLLYEFCAELGLNEQIVEAPHGAPRFVLLGGKLVPVPMS
                     PPAFLSSDLLGWKTKFAILTEVLRTSRPPEPDESIAAFTRRKFSAELLDRLVGPFVSG
                     IYAGDPEQISLRAAFPGIYEAETSSGSVVRGMFRAAKKSQVSVSQAQLSRRPSLLSFR
                     SGNETLPNALADKLGSKLRRNIEVSDIRRTENGFLVRSQKLEASGEISCQRLVVATPT
                     KVTASLLGQLVPRAVPSLREIAYAPVAVVSLGYRREQVRHSLHGFGFLAPRSSLIRTL
                     GTVWNSSLFPSRAPQGQVLLTSFVGGATDPAAADLSDDALSALVHAELAPLLDISGQP
                     VMQHVNLYTRAIPQFNLGHTERLETIREAAKQAPGLWLTGNYWRGPAVGSCIEHALKV
                     AEQVRISYKS"
     gene            2937..3524
                     /locus_tag="VFR42_12820"
     CDS             2937..3524
                     /locus_tag="VFR42_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0370092.1"
                     /translation="MFLAVFTPSESSGAPPDVALVRRLLQRDVNAFGELYDRHSRIVY
                     GLVLRILQQASTAEEVVQDVFLQLWRNAAQYDTSRGPFLPWLLTLSRHRALDHLRLKS
                     ERQRRREDQAHELPAVIAVPDFESHLDEKRRAHRVRELIGALQPQQRRAIELAYFEGL
                     SHSEIAAKLQEPLGTVKSWIRNGLLKLKEGLQTTA"
     gene            3521..4348
                     /locus_tag="VFR42_12825"
     CDS             3521..4348
                     /locus_tag="VFR42_12825"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0370093.1"
                     /translation="MSNNEPTRDPFLELMESYALGALDPEERASLEAHLAAGCAECGE
                     AFEEARWLVSQLAYLAPEAQPSNTLRGRLLKTARTDIANNDAKDEFAPATSRAATPLW
                     MWGAVAASVLFAFYNFYEARSVRQAIRQTQAALDEQVELQKKSARELALARREALILT
                     DPRSVKIPMPAGQKDLPPLQATWHAALGILVSGESLPVPRENRTLQLWLIPKDPGSKP
                     IPSLAIRPDAGGKFHLLVQNPPDTQGDTKALAITEEPAGGSQAPTTAPIWVGAVAGK"
     gene            4483..>5606
                     /locus_tag="VFR42_12830"
     CDS             4483..>5606
                     /locus_tag="VFR42_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEU0370094.1"
                     /translation="MAMGRPKAELVLRPEQREQLESLVSSRSLPAGLVSRARIVLLSA
                     SGKTNMQIASQLGLTNATVGKWRRRFVEQDVSGLHDELRPGRPRPIGDERVAQLVRKT
                     LETKPKDGTHWTIRQIAQQTRLSKSTVHRIWQAFGLQPHRQRQFKLSNDPFFVEKVRD
                     IVGLYLNPPENAVVLCVDEKSGIQALERTQPMLPLGLGYVEGVTHDYRRHGTMTLFAA
                     LDTAKGKVLTQCRQHHRHQEYLGFLREIEKNVPETLDVHVIVDNYATHKHPRVKRWFA
                     ARPRFHVHFTPTYASWLNQVEIWFNRITQQAIRRGTFRSVKELVEKIDQYVQSSNTHA
                     QPFVWTATADSIFAKVERLCDKRNTKPASPATALSVLASA"
BASE COUNT         1192 a         1760 c         1518 g         1136 t
ORIGIN      
        1 ccaacccggc acggcgtcca atggcctgct agaatagaga gcgaaagcac cgctgtgacg
       61 attcagcagc ctagtttcga acaggccaaa cgcgaactcc gcgtcactga agaatttctg
      121 gcccattaca accgtccggg cccgcgttat accagctatc ccaccgcccc ggtgtggaac
      181 gatgcctttg gccccggcga tctggaagaa gtccactcgg ccgccgacgc cgcgcgcagt
      241 cctgtttccc tttacatgca tctgcccttt tgcgagagcc tctgcctttt ttgcgcctgc
      301 aacgtcatca tccaaaaaga caaaaccgtt gctcttccct atttcgacgt tctgaagaaa
      361 gaaatctccc gtgtagcgca agccgtctcg cgccatcgcg acgtcgtgca attccattgg
      421 ggcggcggca cgcccaccta ctcttctccc gcgcaacttg aagatctttt tgcctatacc
      481 aaagagcgat tcacattttc accgcacgcc gaaataggca tcgaagtcga tccccgcgtc
      541 acctcgcgcg aacatctgga aactttgcgg cgcctcggct tcaaccgcct cagtatgggc
      601 attcaggatt tccacgagcc ggtacagaag gccattcacc gcttccagcc gtacgaaata
      661 acgcgcgacc tcattcaatc tgctcgcgca ctcggcttcg acagcatcaa tgtagacctg
      721 atttacgggc tgccgcacca gaccccggat actttcgcgc acaccgtgga ccaaatcatc
      781 ggtctggcgc cggatcgcat cgcgcttttc agctacgcgc acgttccgtg gttaaaaaag
      841 cagcaaggtg ccttcgcgat gtttctcccc gaaggcatgc agaaattcga aattttccga
      901 actgggttgc tgaaatttct ggaggccggc tatctgtaca tcggcatgga ccacttcgcc
      961 agaccgaacg acgaattggc aaccgcgcag acaaatcgga cattgcaccg taactttcag
     1021 ggctacacca caaaggcagg tgcggacctc tatggaatgg gtgtcaccgc catcagcgga
     1081 ttcagcgacg cttacgcgca aaacttccgc gagttgcccg cctgggaaaa agccgtcaac
     1141 gagcgcggca tcgccaccat gcgcggttac catctttcgc aagaagaccg cctgcgccgc
     1201 gaggttatca gccgcctgct ctgccacacg gtgattccca agcgcgagat caatcaaacc
     1261 ttcggcatag acttcgactc acatttcgcc gaggaactgc ttcgcctcga acttccccgc
     1321 gaagacggcc tcctgaccat cgaccgggag gaaatccgca ccacctggct ggggcgaatt
     1381 ttcatccgca acctggccat gcttttcgat ccctatctcg aaaagcagca tctcgacgcc
     1441 aaaccgcttt tttcgaaaac actctgagcc gtggcctgcg caccgctcac cgtcgttatc
     1501 ggcgccggaa tctccggctt ggcttgcgcc tatgccctgc aaaaaaaggg ccaggatgtt
     1561 ctggtcgtgg aagctgcgtc gcgcccaggc ggcgtcattc aatccgtcga aaaagacgct
     1621 tacctcctcg aacaagggcc gcaaagtttc agcggcacgc cattactcta cgaattctgt
     1681 gccgagcttg gtctaaacga gcaaatcgtc gaagcgccgc acggcgcgcc gcgcttcgtc
     1741 ttgctgggcg gaaagctggt gcccgttccc atgtcgcccc cggcttttct gtcttccgat
     1801 ctcctcggct ggaaaacgaa attcgccatc ctcaccgaag tcttacgcac ctcgcgcccg
     1861 ccggaacctg atgaatctat tgccgcgttc acgcgccgca agttttcagc ggaattgctg
     1921 gatcggctgg ttggcccctt cgtctctggc atttatgccg gtgacccgga acagattagt
     1981 ctgcgtgctg cgtttcccgg gatctatgag gcggaaacgt cgtcaggcag cgtggttcgc
     2041 ggaatgttcc gcgcggcaaa gaagtcgcag gtcagcgtgt cgcaagcgca gctaagccga
     2101 cgtcccagcc tgctctcttt ccgctctggc aatgaaactc tgccgaacgc gctcgcggac
     2161 aaacttggtt caaagctgcg gcgcaatatt gaagtcagcg acatccgccg gacagaaaat
     2221 ggatttctcg tgcgctcgca aaaactggaa gcctccggag aaatctcctg ccagcgactg
     2281 gtagttgcga cgccaaccaa ggtgacggct tctcttctcg gacagttggt cccccgggcc
     2341 gtgccttcac ttcgtgagat cgcctacgcc cccgttgcgg tcgtctctct cggctatcgc
     2401 cgcgaacagg ttcgccactc actccacggc tttggttttc tcgcaccgcg ttcctctcta
     2461 atccgtactc tgggcaccgt ctggaactca tccctcttcc ccagccgcgc tccccaaggg
     2521 caagtcctgc tgaccagctt tgtcggcggc gccacggacc cggctgctgc ggatctttcc
     2581 gatgacgcgc tctccgcgct ggttcacgct gaacttgcgc cgctcctcga catcagcgga
     2641 cagccggtga tgcagcacgt caacctgtac acgcgcgcta ttccgcaatt caacctcggc
     2701 cacacagagc ggctcgaaac cattcgcgaa gcagcgaagc aggcgcccgg cttgtggctc
     2761 acgggaaact attggcgcgg ccccgcagtc ggctcctgca tcgaacatgc gcttaaagtt
     2821 gctgaacaag ttcgaatcag ttacaagtcc taaagcagtt cgcagccctt tttgccgcgc
     2881 ctcatccact tggccgcttt tgtcgtatat cttgcaggag acgaggaagc cctaacgtgt
     2941 tccttgcggt cttcacaccc tcggaatcga gcggagcgcc gcccgacgtg gcgctagtgc
     3001 ggaggcttct gcaacgggat gtaaatgctt tcggagaact ctacgatcgc cactcccgca
     3061 tcgtctatgg cctggtgttg cgtatcctcc agcaggcgtc cacggccgaa gaagttgtcc
     3121 aggatgtttt cctgcagctc tggcgaaacg cagcgcagta tgacacctcg cgcggcccct
     3181 tcctcccttg gcttctgacg ctttcgcgcc atcgcgctct ggaccacctc aggctcaaaa
     3241 gcgaacgcca gcgccggcgc gaagaccaag cccacgaact gccggcggtc atcgcagttc
     3301 cggactttga atcgcatctc gacgagaagc ggcgcgcgca tcgcgttcgt gaactaatcg
     3361 gcgcgctgca accgcaacag cgccgcgcca tcgagcttgc ctactttgaa ggtctcagtc
     3421 attccgaaat tgcggcgaaa ttgcaggagc cgctcggcac cgtcaaaagc tggattcgca
     3481 acggtctctt gaaactaaag gaaggattgc aaaccacggc gtgagcaaca acgagccaac
     3541 tcgtgacccg ttcctggagc tgatggaatc ctacgctctc ggtgcgcttg accccgagga
     3601 gcgcgcttcc ctcgaagcgc atctggccgc cggttgcgcg gagtgcggcg aggcgttcga
     3661 ggaagctcgc tggctggttt ctcagttggc ctaccttgcg cctgaagccc agccttccaa
     3721 cacgcttcgc ggccgtctgc tcaaaaccgc gcgcaccgac attgcgaaca acgatgcaaa
     3781 ggacgaattc gctcccgcca cctcgcgtgc ggccactcct ttgtggatgt ggggcgccgt
     3841 cgccgcctca gttctcttcg ctttctacaa tttctacgag gcgcggtcgg tgcgccaggc
     3901 cattcggcaa acccaggccg cgctcgacga acaggtcgaa ttgcagaaga aatcggctcg
     3961 ggaactggcg ctcgctcgcc gggaagccct catcctcact gatcctcggt ccgtgaaaat
     4021 ccccatgccc gcgggacaaa aggatcttcc gccactacag gccacctggc acgccgcact
     4081 cggcattctg gtttctgggg aaagcctgcc cgtaccgcgc gaaaaccgca ctttgcagtt
     4141 gtggctgatt cccaaagacc caggctctaa gccaattccg tctctggcca ttcgcccaga
     4201 cgcgggcggc aagttccatc tgctggtgca gaacccgccc gacactcagg gcgacaccaa
     4261 agctctcgcc atcaccgaag aacccgcagg cggaagccag gccccaacca ccgcccccat
     4321 ttgggtcggc gccgtagctg gaaaatagaa gctagaaaat agaaagggca aatagaagtc
     4381 aggcaatcga cgttttgcca tgttctagtg ttgtgtccca taaataagtg gcaatagtat
     4441 tgctgcgtag atgggcatct ccagtgcaca atgggggtgc ctatggctat gggacgaccg
     4501 aaagcggagt tagttttgcg tccggagcag cgcgaacagc tcgaaagcct ggttagttcg
     4561 cgatcccttc cggcagggct ggtaagccgg gccagaattg ttttgttgag tgcttccggc
     4621 aagacgaaca tgcaaattgc gagccagttg gggttgacga atgccaccgt aggcaagtgg
     4681 cggcggcggt ttgtggaaca ggatgtttcg ggtctacacg acgagttgcg ccccggccgc
     4741 ccacgcccga tcggtgacga gcgtgtggcg cagttggttc gcaagacgct agagacgaaa
     4801 ccgaaagacg gcacgcactg gacgatccgg caaattgcac aacagacgcg attgtcaaaa
     4861 tcgacggtgc atcgcatttg gcaggccttt ggattgcagc cgcaccgaca acgacagttc
     4921 aagctctcga acgatccatt ttttgtagag aaagtacggg atattgtagg gttgtacttg
     4981 aatcccccgg aaaacgctgt ggttttgtgc gtggacgaaa agagcggaat tcaggcactg
     5041 gaacgcacgc agccgatgtt gccgctgggg ttgggttacg tcgagggagt gacccacgat
     5101 tatcgacgcc acggaaccat gacgctgttt gcagccttgg acacggcaaa aggcaaagtg
     5161 ctcacgcagt gccggcagca tcaccgccat caggaatatc tgggtttcct aagagagatc
     5221 gagaagaacg tgccggagac actggatgtg cacgtcatcg tggataacta cgctacacac
     5281 aaacatccgc gagtaaaacg ctggttcgca gcacggccgc gattccacgt gcacttcact
     5341 ccgacctatg cctcgtggtt gaaccaagta gagatctggt tcaaccgcat cacgcagcaa
     5401 gctatccgcc gtgggacgtt tcgcagcgtc aaggaactgg tcgagaaaat cgatcagtac
     5461 gttcagagtt ccaacaccca cgcgcagccc ttcgtctgga ccgccacggc cgattcgatc
     5521 ttcgctaaag tcgagcgact atgtgataag agaaacacga aacccgcgag tccggcgacc
     5581 gctttgagtg tgctggccag cgcctt
//
LOCUS       DASTPM010000725         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_401409, whole genome shotgun sequence.
ACCESSION   DASTPM010000725 DASTPM010000000
VERSION     DASTPM010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401409"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VFR42_12835"
     CDS             complement(<1..1289)
                     /locus_tag="VFR42_12835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU0370095.1"
                     /translation="MSVRPPSLLPGDPFLSIHAVRVYVRNVDRSLQFYVEKLGFRLVI
                     DTKLQSGERWVAVSPPDGTTILALVAPKPKTAEHKLIGRATQVVLVTSEVTTKFQEWS
                     RRGVRFLSTPRLRRIKYDHTPADAAGNTSTRLLGRETPVWGTASARFSDIDGNVFSLV
                     SFDELTHAVEADRRAAAEKLEAERYVARELEIAKQVQSRLFPQCLPALSTLDYAGSCI
                     QARQVGGDYYDFLDLGSPQRLDRGATRALDAAAWHLAFVLADVCGKGIAGALLMANLQ
                     ANLRSRYALALDDLPRLLKSVNQLFYENSPDDRYATMFFGIYDDRTSELEYANCGHNA
                     ALVFRADGSLERLASTSTVIGLFQRWECATQRVALQADDLLVIYTDGVTESLNHDYEE
                     FGEERLIESVHRHRGLAPQTLMAAIVEEVRAFSPHEQ"
     gene            complement(1336..2004)
                     /locus_tag="VFR42_12840"
     CDS             complement(1336..2004)
                     /locus_tag="VFR42_12840"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU0370096.1"
                     /translation="MQRARTSVSQEDYLKAIWELLEEGQPPISARLAEELSVTPPAVT
                     AALKRMRRDDLVSVARDGRIDLTRKGRAVAERMALRHQLAELLLTDVIGLPWSKAHDE
                     AERLEHAISPEVEALLLKRFAGKKACPHGLPLRGGSAQLRKQGAVLLSDLNAKDRAEV
                     ICVYEKDPRFLEFLDELKLHPGAQLAVARREYDETLTLQVAGKTLHLGKPATSRIWVR
                     QLPA"
     gene            complement(2024..2461)
                     /locus_tag="VFR42_12845"
     CDS             complement(2024..2461)
                     /locus_tag="VFR42_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEU0370097.1"
                     /translation="MNDSSKKLSVLFVCIGNSCRSPMAESIALRDAADLFECASAGLN
                     ALGVVQRQTLQTLETNGYPTDGLYSKPILNDAWADADLVINMSGYPRETAFARGDWHK
                     VEDWDVEDPYGLDPNVYQRIFEDIRTRIETLAARLRERKRPRR"
     gene            2581..2733
                     /locus_tag="VFR42_12850"
     CDS             2581..2733
                     /locus_tag="VFR42_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370098.1"
                     /translation="MSFRSVFMALAIAFALILGAFLIQRARPRIETDQPNADFVKATG
                     KCAECH"
     gene            2767..>3181
                     /locus_tag="VFR42_12855"
     CDS             2767..>3181
                     /locus_tag="VFR42_12855"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU0370099.1"
                     /translation="MSKHASQGVSCLDCHQPQKGQEPDRQDHNGFVITAHITPANCRT
                     CHQQIYQEFTRSRHAAPSWAAVYGEAALTAEQVDFAEKLHPGYVKRPANALAKLEGPS
                     AIAGGCAQCHSIGKPQADDTTGNCTACHTRHTSSVA"
BASE COUNT          652 a         1052 c          842 g          635 t
ORIGIN      
        1 tgctgttcgt gcggactgaa ggcgcgcact tcttccacaa tcgccgccat cagcgtttgc
       61 ggagcaagtc cacgatgacg atgtaccgat tcgatcaaac gctcttcgcc aaactcttcg
      121 taatcatggt tgagcgattc ggtcactccg tccgtgtaga tcaccaacaa atcgtcggcc
      181 tgcaacgcga cacgctgcgt cgcgcactcc cacctctgga acaagccaat gactgtcgac
      241 gttgaagcca ggcgctccag cgaaccgtcg gcccggaaga ccagcgccgc attgtgtccg
      301 caatttgcgt actccagttc gctcgttcgg tcgtcgtaaa tgccgaagaa cattgtggcg
      361 taacgatcgt ccggcgagtt ttcgtaaaac agttggttca cggatttcag caaccgcgga
      421 aggtcgtcca gcgccaacgc gtagcggctg cgtaaattgg cctgcaagtt ggccatcagc
      481 agcgcgccgg cgattccctt tccgcaaacg tcggccagca caaatgccag atgccaggcc
      541 gcggcatcga gcgctcgcgt cgcacctcga tcgagtcgct ggggcgagcc gagatccaga
      601 aagtcgtagt agtccccacc cacctggcgc gcttggatac agctgccggc gtaatcaagc
      661 gtcgaaagcg ccggcaggca ctgcggaaac aagcgcgact gcacctgctt cgcgatttcc
      721 agttcgcggg cgacgtaacg ctcggcttcc aacttttccg cagccgcgcg gcgatctgct
      781 tccaccgcgt gcgtcagctc atcaaagctc accagcgaaa aaacgtttcc gtcgatatcg
      841 ctgaagcgcg ccgaggcagt accccacacc ggcgtctcgc ggccaagcaa acgcgtggaa
      901 gtgtttccgg cggcatctgc gggagtgtgg tcgtacttga tccgccgcaa ccgcggcgta
      961 ctcaaaaaac gcacaccgcg ccgcgaccac tcctgaaatt tcgtggtgac ctccgaagtc
     1021 accagcacta cctgcgtggc gcgcccgatc agcttatgct ccgccgtctt aggcttgggc
     1081 gcgaccagcg ccagaatggt cgtcccgtcc ggcggcgata ccgcgaccca ccgctcgccc
     1141 gactgcaact tggtatcgat gaccagccga aagcccagtt tctctacgta aaactgcaag
     1201 ctgcggtcca cattgcgcac gtacacgcgc accgcgtgga tgctcagaaa tggatcgcca
     1261 ggcagcagcg aaggcggacg gacactcatg caccgaatta taagcagtta gcggttcgaa
     1321 gttttagtgt ttccgtcagg cgggaagttg ccgcacccag atgcgcgacg tcgcgggctt
     1381 gcccaagtgc agcgttttcc cagcgacctg caaggtcaag gtctcatcat attcacggcg
     1441 cgctaccgct aactgcgccc cggggtgcag cttcagttcg tcgagaaatt ccagaaagcg
     1501 gggatctttt tcgtaaacac aaatcacttc tgcgcggtcc ttggcattca aatcggacag
     1561 tagcaccgct ccctgcttgc gtagctgcgc cgatccgccg cgcagcggca acccatgcgg
     1621 gcacgctttt tttcctgcga atcgcttcag cagcagcgct tccacttcag gagaaatcgc
     1681 gtgttccagg cgttctgctt catcgtgagc cttggaccac ggcagaccga tcacatcggt
     1741 caataacagt tccgccagct ggtgccgcaa agccatccgt tccgcaacag ctcgcccttt
     1801 gcgcgtcaga tcgattcgcc cgtcgcgcgc cacgctcacc agatcgtcac gccgcatacg
     1861 tttcagcgcc gcggtcactg ccgggggagt tacgctcaat tcctcagcaa gacgcgcgct
     1921 gatcggcggc tggccttctt ccaaaagctc ccagatcgcc ttcagatagt cttcttgcga
     1981 aacgctggtc ctggctcgct gcaatggctc tccgtaaacg gcgttatcgc ctaggtcgct
     2041 tgcgttcgcg caatcgcgcc gcgagcgtct ctattctagt ccgaatgtcc tcaaagatgc
     2101 gctgatacac attcggatcc aagccgtagg gatcttccac gtcccagtcc tccactttgt
     2161 gccagtcgcc gcgcgcaaac gctgtctcgc gcggatagcc gctcatgttg atcaccagat
     2221 cggcgtccgc ccaagcatcg ttcaggatcg gtttggaata caacccatcg gtcggatatc
     2281 cattcgtttc gagcgtctgc aaggtttgcc tctgcacgac gcccagcgca ttcaggcccg
     2341 cgctcgcgca ctcaaacaaa tccgcggcgt cccgcaacgc gatcgactcc gccatcggac
     2401 ttcggcagga attcccaatg cacacaaaca gcacactcag ttttttcgat gagtcgttca
     2461 tacgctgtaa aagcaggtta acccaacacg ctgcaatcct tgccagcaca ataactctgt
     2521 gatatcaata cgttcccttc cttgggagtt gcgttcccca cgcttaagcc gggaggattc
     2581 atgtcctttc gctccgtttt tatggcgttg gccatcgcct tcgctctaat ccttggcgcg
     2641 tttctgattc agcgtgcgcg tccgcgtatt gagaccgacc agcccaacgc ggacttcgtc
     2701 aaagccaccg gcaaatgcgc cgaatgccat tgacgccagc aatactccat cgttcatgaa
     2761 tacgagatga gtaagcacgc cagccagggc gtcagctgcc tcgactgtca tcagccgcaa
     2821 aaaggacagg agccagatcg ccaggaccac aacgggttcg tcattaccgc ccacattacg
     2881 ccggccaact gccgcacctg tcaccagcag atctaccagg aatttacgcg cagccgtcac
     2941 gccgcgcctt cttgggctgc cgtttatggc gaagcggcgc tgaccgccga acaggtcgac
     3001 ttcgcggaaa agctgcatcc tggatacgtc aagcggcccg ccaacgctct tgccaagctc
     3061 gaagggccct cggccattgc gggcggttgc gcgcagtgcc actccattgg caagccccaa
     3121 gccgacgaca ccaccggaaa ttgcaccgcc tgccacactc ggcacaccag ctcggtcgcc
     3181 g
//
LOCUS       DASTPM010000726         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_401474, whole genome shotgun sequence.
ACCESSION   DASTPM010000726 DASTPM010000000
VERSION     DASTPM010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401474"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            624..2021
                     /locus_tag="VFR42_12860"
                     /pseudo
     CDS             624..2021
                     /locus_tag="VFR42_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057901383.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            2229..3878
                     /locus_tag="VFR42_12865"
     CDS             2229..3878
                     /locus_tag="VFR42_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370100.1"
                     /translation="MPCDELTSYYTDLLDGSYSCLDRLVLNANFAPCYSAGGFRWWWR
                     QLHNGSDEELDDAHLMRMAGHFGRRLRGWAKAHRVPVIDCRREEDKHELAEQYLQNHP
                     EVGGLFLILVGRAVATVWTVHRSHGGVILNLEAKPRYINHYSFHILDPDWGHITIKMS
                     GHPPFGAQIMLNGHEYVACRAKKVGIPFTKEGNCFTLVSKPADLAKVADTLSEVRTTG
                     RLRQVCERWIYTSCLCFALDLQDQQRSGFRYQYSIYQVEYSRNLLFHRGGQMEQIFQG
                     LIDRTRARLNIQNLKTIFGFKSRPHRDRKGKPPRLEAVVETPSYDLTIFKLHFGKLTL
                     KAYTKGEHVLRFEAIAHNTKELRCGRLLDRFPQIVLRLRQILQQFLSNLYYMDATFIS
                     DETLDQLPTSSQIGNTRVGGIDLNKPRTRAVLSAILSLACSPDGFTAGHVADRVRSTP
                     RMKDSNYDVRRAAYDIKKFRGKGLLSKLPNSHRYAIPQQAVRTIAALVILREKLLRPI
                     LASVHKPRNSHQPKDCTAIDQHYENLRQDMFTLMHDLRIAA"
BASE COUNT          937 a         1129 c         1120 g          830 t
ORIGIN      
        1 tgtcaaagtt cttctctcta gctagcaggc tgcggaaaaa cgacggtacg agttaacatg
       61 ctgtccaagg ctcgtgattt gttgtaacta agaggagagt tttggttgca tgcgctggag
      121 tctgtagtcc ttttcgggcc agccaacaca cttcggcctt ggcttacgtc gcgttctgcg
      181 aaactcggac tcgaaaaccg cggcagtcgt gtccccatac ttttcaccag tcctcgcgtg
      241 gtgtaaatca actccgtccg caccgatacc aacgcgtccg ggtgcggcca atggcctttt
      301 tctttcgatc tccttttgcc ttacccgaca tggccggcgc atccagcact gctttcccgg
      361 tcagctttcc cagtacctct gccattactt gtttcacctc tcgctgcacc ggccctaacg
      421 acttcgttcg cttcaaggtt ggttcactcc gctgcgaaga ttagctactt tccgtcgcaa
      481 aagtgaggcg atgagagcag atggatgctg aggggagacc cggagggctg ggtcagaatg
      541 ctcgctttga ggttatccga gaaagtaata aaacattttc gaagcatatt gttttagagg
      601 ggaagtgcct taggatgggg ttcatgacca agctacgaca ccaacaacca tcgctgtggc
      661 acccaggttt aacgaaggac atcgaggact tgtgggaacc gtggatgcgt ctggtcgacc
      721 gtctgctgga agatgagcaa ctgctggaca ccatttatga ggcgcaaggc gaacgctatc
      781 cggagagccg caggcggggg cgcgagccaa cgccggccga agtgttgttg cgtctgctgt
      841 tgttgaaaca cattcgaaac tggagctatg acacgctgga gcgagaagtg cgcgccaatc
      901 tggtgtatcg ggcgtttacg cggatcggag acgagaaggt gcccgatgcg aaaactctgg
      961 cgcggctggg gcaactcatc ggacccgaag tgattgagga gttgtaccag cggatggtgc
     1021 agttggcgca agaacacggg gtgacgggtg ggcgaaagct gcgggtcgat accacggtgg
     1081 tggaaaccaa catccactat cccaccgaca gcagtttgtt gggggacggc acgcgggtac
     1141 tgacgcggac gatgaagaag atcgagcaga aggccggggg gctgaagcgc aaggtacggg
     1201 atcgcatgcg gagtgtgcgc aagcgtgttg tggcgattgc cctgtcgacc cggtacctgg
     1261 gtgcataagg agaagcgcgg aggaagaaac agtatagaga actactgagt ctaacccgga
     1321 aaactgtgaa tcaagcgcaa cggatattag aagaggtgaa ggcagggccg cgagctcgtc
     1381 gcattcccct gcgaggctta gtggaatcgc tggcgacgat gatggggcgg gtgcagcaag
     1441 tggtgaaaca gacccggctg cgaatttttg ccggggtgac caagtcacag gaaaagatgg
     1501 tgagcgtgtt cgagtcgcac ccggaaatca ttcgcaaagg caaggccagc cagccgaccg
     1561 agtttggcaa gctggtcaag atccaggaat cggagaatca aatcatcagc catttcgaag
     1621 tttttgccga acggcccagc gaccggcagt tgttagtacc gtcgatcgag atccaccagc
     1681 ggagattcgg ccggattccg gcttgggtgg ctgcggacgc gggatttcac tcgcagcaga
     1741 atgaaaacgc tgcccgcgcc ctgggagtgc gatggttttc cgtgccgaat cagaagacca
     1801 agagtgcgga acgcaaacga ctttatcggc agcgctggtt tcgtcgtgga cagcggtggc
     1861 ggaccggatg tgagggccgc atccgcgctc tgaaacggag acatggaatg cgtcgctgct
     1921 tgtatcgcgg gttccacggc atgcagcgtt gggcaggact gagtgtgatc gccgataatg
     1981 tgatccagat cgggcgctgt ctcgcgctgc aggggacctg aatggccgac ctctcggtca
     2041 cgcttgatcg aactgagcag ccggaactga tcagcaaaga cggctccgtt tcgttcgcac
     2101 ctgaccagag ccgcctcaga aacggcattt ttgcgacgga aagtagctaa tttgtttcgg
     2161 cgacggtgcg ccgaaaaaat ggccctgcgg gcagttgctt gccgagagag atgccggtat
     2221 cttcgtccat gccctgcgac gaactcacca gctactacac agatctgctc gatggcagct
     2281 acagctgcct cgatcgtctt gttctgaatg ctaacttcgc accctgttac tcagccggtg
     2341 gcttccgttg gtggtggcga cagctacaca acggttctga tgaggaactg gacgatgctc
     2401 acctgatgcg gatggcaggg cactttggtc gtcgcctgcg tggctgggcc aaagcccatc
     2461 gagttccagt catcgattgc cgccgcgaag aggacaagca tgaactcgca gagcagtatc
     2521 ttcagaacca tcccgaggtc ggggggctct ttctaattct ggtgggccga gcggtggcca
     2581 ccgtgtggac agtgcaccga tcccacggcg gtgtaatcct gaatctcgaa gccaagccac
     2641 gctacatcaa tcactactcc ttccacatct tggatcccga ctggggccat atcaccatca
     2701 agatgtcggg ccatccccca tttggtgcac agatcatgct caacggccat gagtacgtgg
     2761 cctgccgagc caagaaggtc ggcatcccct ttacaaaaga ggggaactgt ttcaccctcg
     2821 tctctaagcc cgcagatttg gccaaagtcg cagacacctt gtccgaagtt cggactacag
     2881 ggcgcttgcg ccaagtctgc gagcgttgga tctacacttc ctgtctctgt ttcgcgctgg
     2941 atctccaaga ccagcaaagg agcggatttc gctatcaata ctcgatctat caggtggagt
     3001 acagccggaa tctactcttc catcggggcg gacagatgga gcagatcttt cagggactaa
     3061 ttgatcgaac ccgagcccgc ttaaacattc aaaatctgaa aaccatcttc ggcttcaaga
     3121 gccgtcccca tcgtgaccgc aaaggcaaac cacctcggtt agaagccgtg gtcgagacac
     3181 ctagttatga cctgacgatc ttcaagctac acttcggcaa gctgactctg aaggcataca
     3241 ccaaaggcga acacgtactt cgtttcgagg ccattgctca caacaccaag gaactccgct
     3301 gcggccgact cctggaccgt ttccctcaga tcgtcctccg actccgacag atcctccagc
     3361 agttcctcag caacttgtac tacatggatg ccaccttcat cagcgatgaa accctcgatc
     3421 aacttcctac ttcttcgcaa atcggcaaca cccgagttgg cggcatcgac ctcaacaagc
     3481 ccagaacccg agccgtactt agtgccatcc tctccctggc ttgttccccg gatggattta
     3541 ctgctgggca cgtggccgac agagttcgct ccacacccag gatgaaggat tccaactacg
     3601 acgttcgtcg cgccgcctac gacatcaaga agttccgtgg caaaggcctg ctcagcaaac
     3661 tccctaactc ccaccgatac gctattcctc aacaagccgt ccgtaccatc gcagcgctcg
     3721 tcatcctacg agaaaaacta ctccgtccca tcctcgcttc cgttcacaaa ccccgaaaca
     3781 gccaccaacc caaagactgt actgccatcg accaacacta cgagaacttg cgccaagaca
     3841 tgttcactct catgcacgac cttcgcatcg cagcctagca atcagcaaga ttttgtcggt
     3901 cttcattccc caaagcgcct agtcgggtgg ccaaaagcga taaactcgtt gaaaggagca
     3961 gccatgacgg acaatcgaaa aaaacaacaa aggaacgacg cgcatcccgg agacat
//
LOCUS       DASTPM010000727         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_689881, whole genome shotgun sequence.
ACCESSION   DASTPM010000727 DASTPM010000000
VERSION     DASTPM010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689881"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFR42_12870"
     CDS             complement(<1..386)
                     /locus_tag="VFR42_12870"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU0370101.1"
                     /translation="MAGALDNKQILVKIFIGIFVGLIGISMLLYLVPQGPGTGSDADG
                     SDVVAKVGNQTVSLADVRQQLSEIQRRNQVPKALEGLYARQILNQLVFTKEIEFEAQR
                     LGIKVTNEEIADRVKQYLPTAFSGGNP"
     gene            complement(723..1118)
                     /gene="cdd"
                     /locus_tag="VFR42_12875"
     CDS             complement(723..1118)
                     /gene="cdd"
                     /locus_tag="VFR42_12875"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU0370102.1"
                     /translation="MRGDYEALITAAIQARENAHAAYSNFRVGAALRASSGRIFGGCN
                     VENATYGLTVCAERVAILKAISEGERGFDAIAVVADTDSLTAPCGACRQLIWEFCGDV
                     PVILANLKGKTEVIAMRGLFPKPFDASSL"
     gene            complement(1115..1978)
                     /locus_tag="VFR42_12880"
     CDS             complement(1115..1978)
                     /locus_tag="VFR42_12880"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU0370103.1"
                     /translation="MPVKKQSAKPAPEFLHAEAAAKFIHRKTKLRPKIALVLGSGLGA
                     FADEFSDAAKIPYKKIPHFPRSTAIGHAGQLVLGTVDGIPVAGMQGRVHLYEGYSAKQ
                     VAFPMRVFSRMGVKAVILTNAAGGLNLNYSEGALVALRDHINLQGANPLIGPNDDRFG
                     PRFPDMTRAYDPDFRRFVAEEGKKLKLNLHEGVYLACAGPNYETPAEIHGFRTLGADL
                     VGMSTVPEVLAARHSGIRVLGISCVTNMAAGITGKTLSADEVFETGARVKTQFIALLR
                     AIIPRIAEVVA"
     gene            complement(1983..3215)
                     /locus_tag="VFR42_12885"
     CDS             complement(1983..3215)
                     /locus_tag="VFR42_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEU0370104.1"
                     /translation="MGRFTGILGMAVILALAWAFSTDRRAIKLKTIAWGLGLQLLLGF
                     FVLRSSIGGKIFGFLGNGANKLLSFSYAGSEFVFGDIGFPKEISRLGFSFAFQVLPTI
                     IFIAAFFAVLYHIGVMQLIIRAAAWIMTRVMGASGAESLNVAASIFMGQTEAPLTIRP
                     FLPKLTKSELMCVMTSGMAHISGGMMAGYIQVGGADPKNLLTAVIMTAPGTLLMAKML
                     VPETEEPLTAGRVEMPQMEKESNILGAIARGTSDGLHLALNVGAMLITFIALLALLNA
                     MMGGIHNWPHMSWFPPSLQTIFGWVFAPVAWLIGIPWHDAPAIGNLLGTRMVINEFVA
                     FGQLGPMKGSLDPRSFTVATFALCGFANFSSIGIQIGGIGALAPERRNDLAKFGVRAM
                     MAGTMANLMSASIVGILS"
BASE COUNT          725 a          851 c         1138 g          654 t
ORIGIN      
        1 gggttgccgc cgctaaatgc ggtgggaagg tactgcttca cgcggtcggc gatttcttcg
       61 ttggtcacct tgatgccgag tcgctgagct tcgaattcga tttccttcgt gaagaccagt
      121 tgattcagaa tctggcgcgc atagaggcct tcgagagcct tgggaacctg gttgcgccgc
      181 tgaatttcgg aaagctgctg acggacatcc gcgagtgaga cggtctggtt gccgactttc
      241 gccacgacat cggagccgtc ggcatcgctg ccggttccgg gcccttgcgg cacgaggtac
      301 agcaacatac tgatgccgat gaggccaacg aatatcccga tgaagatttt caccaagatc
      361 tgcttattgt ccaaagctcc agccatccgt agcgtctccc ctgagagtgg tcaaacttaa
      421 gattatagac gagcgagagg ttgcgcccaa atcaatttga atggctgcgg cgagcttcgt
      481 aacctaccgg caaatcagag tctgcgttag aacaatcgct gcgctaagcg atctaccagc
      541 tgctgaaccc gatagggatg gtggctacca ggacccacca gaaaggcaag gcagaggcag
      601 cgatgagacc gagaatggcg aaggtggccc agaacacttt gcgctgcatg gattgatttt
      661 agcaatggat gctgcgaaac ggcatcgcac tggagccgag gtgctctcga aacgttaacc
      721 gactacaagc tggaagcgtc gaacggcttg gggaaaaggc cgcgcatggc gatgacttcg
      781 gttttgcctt tgagattggc gaggatcacg gggacgtcgc cacagaattc ccagatgagc
      841 tgacggcatg cgccgcaggg agcggtgagc gagtcggtat cggcgacaac ggcgatggcg
      901 tcaaagccgc gttcgccttc gctgatggct ttgaggatgg cgacacgttc ggcgcagacg
      961 gtgagaccgt aagtggcgtt ttcgacgttg cagccgccga agatgcggcc ggagctggct
     1021 cgaagggctg cgcctacgcg aaagttggag taggccgcgt gggcgttttc gcgggcttgg
     1081 atcgccgcgg tgatcagggc ttcgtaatcg cctctcacgc gaccacctcc gcaattctgg
     1141 gaataatcgc tcgcaggaga gcgatgaatt gtgttttcac acgggcgccc gtttcaaaga
     1201 cttcgtcggc ggagagcgtt ttgccggtga tgcctgcggc catgttggtg acgcaggaga
     1261 tgcctaggac acggatgccc gaatggcggg ccgcgagtac ttcgggaacg gtggacatgc
     1321 cgacgaggtc ggcgccaagc gtgcgaaagc cgtggatttc ggcaggcgtt tcgtagttgg
     1381 ggccggcgca agctagatag acgccttcgt ggagattgag tttgagcttt tttccctctt
     1441 cggcgacgaa gcggcggaag tcgggatcgt aagcgcgcgt catgtcgggg aaacgcgggc
     1501 cgaagcggtc gtcgttgggc ccgatgaggg gattcgcacc ctgaaggttg atgtgatcgc
     1561 gtagggcgac cagcgcgcct tcggaataat tcagattgag gccgccggcg gcgttggtga
     1621 ggatgacggc tttgacgccc atgcgggaga atacgcgcat cgggaacgcg acctgtttgg
     1681 cggagtagcc ttcgtagaga tgaacgcggc cttgcatacc ggcgacgggg atgccgtcga
     1741 cggttccgag aacgagctgg ccggcgtggc cgatggccgt ggagcgtggg aagtgcggaa
     1801 tctttttgta gggaattttc gcggcgtcgg agaattcgtc ggcaaaggcg ccgaggccgg
     1861 atccgaggac cagagcgatt ttagggcgca gcttcgtctt gcggtggatg aatttggcgg
     1921 cggcttcagc gtgaaggaat tcgggcgctg gtttggcgga ctgtttttta actggcatga
     1981 gtctaactca aaatgccgac gatggaggca gacatcagat tggccatggt acccgccatc
     2041 atggcgcgca cgccgaattt tgccagatcg tttcggcgtt cgggagcgag cgcgccgatt
     2101 ccgccgatct ggatgccgat ggagctgaag ttcgcgaagc cgcacaacgc gaaagtggcg
     2161 acggtgaagg agcgcgggtc gagcgagcct ttcatcgggc caagctggcc gaacgcgacg
     2221 aattcgttga tgaccatccg cgtgccgaga agattgccga tggccggcgc gtcgtgccag
     2281 ggaattccga tgagccaggc gaccggcgcg aagacccaac cgaaaatggt ttgcagactg
     2341 ggcgggaacc aactcatgtg cggccagttg tgaataccac ccatcatggc gttgagcaac
     2401 gcgagaagcg cgataaaggt aatcagcatg gcgccgacgt tgagcgcgag gtgcaaaccg
     2461 tcgctagtgc cgcgcgcgat cgcgccgaga atgttggact ccttttccat ttgtggcatt
     2521 tcgacgcggc cggcggtgag cggctcttcg gtttccggga ccaacatctt agccatgagc
     2581 agcgtgccgg gggcggtcat gatcaccgca gtgagaagat tttttggatc ggcgccgccg
     2641 acctggatgt aacccgccat catgccaccg gagatgtggg ccatgccgct ggtcataacg
     2701 cacatgagtt cggatttcgt gagtttcggc aaaaaggggc ggatggtgag cggagcttca
     2761 gtttgaccca tgaagatgct ggcggcgacg ttgagagact cggcgccgct ggcgcccatg
     2821 acgcgcgtca tgatccacgc ggcggcgcga atgatgagtt gcattactcc gatgtggtag
     2881 agaacggcga aaaacgcggc gatgaagatg atggtgggca atacctgaaa ggcgaaggaa
     2941 aagcccaggc gtgaaatttc tttggggaat ccgatgtcgc cgaaaacgaa ttccgaaccg
     3001 gcataagaaa agcttagaag tttattggcg ccgttgccaa ggaagccgaa gatttttccg
     3061 ccgatgctgg aacgcaagac gaagaaaccg agcaacaatt gaagaccgag tccccaagcg
     3121 atggtcttga gcttgatggc gcggcgatcg gtggaaaagg cccacgccag ggcaaggatg
     3181 accgccatgc cgagtatgcc ggtgaagcgt cccatgcagg ctccttttcg ggaagagaga
     3241 ctctagcagg ttcggggcag cgagcgaaag cgctgtaagg aaggtgaaag gcagcgagca
     3301 gagggtgagc cagggaactg ccaatccgga cagctgtacc aatcattcct tgaccggcgt
     3361 ggaagcgg
//
LOCUS       DASTPM010000728         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1324029, whole genome shotgun sequence.
ACCESSION   DASTPM010000728 DASTPM010000000
VERSION     DASTPM010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324029"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFR42_12890"
     CDS             <1..369
                     /locus_tag="VFR42_12890"
                     /inference="COORDINATES: protein motif:HMM:NF044836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370105.1"
                     /translation="IHRMQPLSWSSQMMTISLLVACRYFSTERWRIAEHFTARPKTDA
                     FNLLVVLSGSGEIRYSVKKARYKQGECWFMPASLKHYELVPSGESVFLRTFVPDLEVL
                     RSNLRSLGVTDSQIAEVVVE"
     gene            375..1487
                     /locus_tag="VFR42_12895"
     CDS             375..1487
                     /locus_tag="VFR42_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEU0370106.1"
                     /translation="MARETLPIHAVILAGGRGTRFWPRSRTKTPKQLLNIAGKGTMLE
                     QTVARFRSLVPPDRIWTVTNVEQENALRKQLPQAARKRVLVEPAGRNTAAAIALAAFH
                     VRKAAKGEALMAVLPADHYIKDVRAYQKIVRTALEVATKPGRMVVLGIPPTHPETGFG
                     YIERKEATIGADGTPVYPVRRFAEKPALETAKAYLASGNYQWNAGMFFWRVSTFLEAL
                     QKHLPATYSQLEKVAASIGTAKYAGRLRTIYPKLENISVDYAILERATAETTEPQVFV
                     VPAQVGWSDIGSWAAVYELLAQKPGEDIFVGKGHSIDASGNYFSSNGKFVAAIGVKDL
                     IVVETPDALLVCPRERAQDVGKFVKWLEAEKLTRLL"
     gene            1567..2973
                     /locus_tag="VFR42_12900"
     CDS             1567..2973
                     /locus_tag="VFR42_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HEU0370107.1"
                     /translation="MSAIKFGTDGWRGVIAEDFTFANARMVAEAISRYVVRGEDARKG
                     VIVGYDHRYTSDWVARRVAEVISSAGTPVWLTDRPCPTPAVSYLVRSRGAAGGIVVTA
                     SHNPYRWNGIKYKASYGSSALPSIVAQVESELQFVESAEIPQLPLRPELIQPLDPRKP
                     YLDALEKLVDWERIRKAKFRFAFDAMHGAAAGLLTELFRRNGVECHEIRGVRDPRFGG
                     AHPEPIEPHIAPLQKEVAAGKFDAGFAADGDGDRIGAVDRSGAFVNPHQIFSLLVWHL
                     AGSRNLPGEIAKTFSVTKLIDKLAAKLGRKLHEVPIGFKYICELMLEQNILIGGEESG
                     GIGTSLYLPERDFTVSALFLAELMAWHGKSLGQLLDELHKEFGEHRYGRVDLDVTTQQ
                     KEKAMAWFGNGMKKIADWPVARSENMDGIKLYLGDVGWVMVRASGTENLLRVYAETSR
                     SETTRKVLSAVTSRIQEL"
BASE COUNT          708 a          778 c         1012 g          615 t
ORIGIN      
        1 attcaccgga tgcagccgct gagctggtcg tcgcagatga tgacgatttc actgttagtg
       61 gcgtgccgat atttttccac ggaacgctgg cggattgcgg aacacttcac agcacggccc
      121 aaaaccgatg cgttcaatct acttgtggtg ctctctggga gcggcgaaat tcgctacagc
      181 gtcaagaagg cgcgctacaa gcagggcgaa tgctggttca tgccagcttc gctgaagcac
      241 tatgagctgg tcccctcggg tgaatcagta tttctgcgta cgttcgtgcc cgaccttgag
      301 gttttaagga gcaacctgcg aagcttggga gtcaccgatt cccaaatcgc ggaagtcgtg
      361 gtcgaataat tgacatggcg cgcgaaacac ttccgattca tgcggtgatt cttgcgggcg
      421 gacgtggaac ccgattctgg ccacgcagcc gaacgaaaac tcccaaacaa cttctgaata
      481 tcgccggcaa aggaacgatg ttggagcaga cagtcgctcg attccggtcg ctggttcctc
      541 cagaccgcat ctggacggtg accaacgtcg aacaggaaaa cgcgttgcgc aagcaactgc
      601 cgcaggctgc gcggaaacgt gtgctggtgg agccggcagg gagaaacacg gcggcggcga
      661 ttgcgctggc ggcgtttcac gtaagaaaag cggccaaggg cgaagcactg atggcggtgc
      721 tacccgcgga ccactacatc aaagacgtac gtgcgtatca gaagattgtt cgcacggcgc
      781 tggaggtcgc cacgaagccg ggacgcatgg tggtgctggg gattccgcca acgcatccgg
      841 aaaccggatt cggctacatc gagcgcaagg aagcaaccat cggggcggat ggaacgccag
      901 tgtatccagt gcgacggttc gcggagaagc cggcgctgga aaccgcgaag gcgtatctgg
      961 cttcgggaaa ttaccaatgg aacgctggaa tgtttttctg gcgggtgtcc acgtttttgg
     1021 aagccttgca gaaacatctg cccgcgacgt attcacaact ggaaaaggtc gccgcttcga
     1081 ttggaaccgc gaaatacgcg ggccggctac ggacgattta tcccaagctg gaaaatattt
     1141 cggtggacta tgcgattttg gagcgcgcca cggcggaaac gacggaaccg caagtgtttg
     1201 tcgtcccggc gcaagtggga tggagcgaca tcgggtcgtg ggccgcagtc tacgaattat
     1261 tggcgcaaaa accgggtgaa gatattttcg taggcaaggg ccactcgatc gacgcgagcg
     1321 gaaattattt ttcgagcaac ggaaagtttg tggcggcgat cggcgtaaag gacctgattg
     1381 tggtggaaac gccggatgcg ttgctggtgt gcccacgcga acgcgcccag gatgtgggaa
     1441 agtttgtgaa gtggctggaa gcagaaaagc tcacgagact gctttgacca ctcggggctc
     1501 gctggaccgt ccaattcggc tgttttccct tccctgccct aacatatact cgcaaggctg
     1561 actggaatga gcgcgatcaa gtttggtacc gacgggtggc gcggcgttat cgccgaagat
     1621 tttacatttg cgaatgcgcg aatggtggcg gaagcaattt cgcggtacgt ggtgcggggc
     1681 gaagatgcac gaaagggagt gatcgtcggc tacgaccacc gctatacgtc ggattgggtg
     1741 gcgcgccgtg tggcggaggt gatttcgtcg gccggaacgc cggtgtggtt gacggatcgg
     1801 ccatgcccta cgcctgcggt gtcgtatctg gtgcgaagcc gaggagcggc cggcggaatc
     1861 gtggtcacgg ccagccacaa tccgtaccgc tggaacggga tcaagtacaa ggcaagctat
     1921 ggcagcagcg cgctgccgtc gatcgtagcg caagtcgaaa gcgaattgca gtttgtggaa
     1981 tcggcggaaa ttccgcagtt gccactgcgg ccggagttga ttcagcctct cgatccgcgg
     2041 aagccttatc tggacgcgct ggaaaaactg gtggattggg agcgcatccg gaaggcaaag
     2101 ttccgctttg cgtttgatgc aatgcacggc gccgcggcag gattgttgac ggaacttttc
     2161 cggcggaacg gagtggagtg ccacgaaatt cgtggcgtac gcgatccaag attcggcggg
     2221 gcgcatcccg agccaatcga accgcacatt gcgccgctgc aaaaagaggt ggcggctggc
     2281 aagttcgatg caggttttgc ggcggatggc gatggagatc gcattggggc agtggaccgc
     2341 tccggtgcgt tcgtgaatcc gcaccagatc ttttcgctgc tggtatggca cctggccggg
     2401 tcgcgaaact tgccgggcga aattgccaag acattttcgg tgaccaagct tatcgacaag
     2461 ctggccgcga aacttggccg caagctgcac gaggtgccga tcggcttcaa atacatttgc
     2521 gaactgatgc tggaacaaaa cattttgatc ggcggcgagg agagcggcgg aattgggacg
     2581 agtctttatc ttcccgaacg cgatttcacc gtgtcggcgt tgttcctggc ggaactgatg
     2641 gcctggcacg gcaaatctct aggacaactg ctggacgaat tgcacaaaga gtttggcgaa
     2701 caccgatacg ggagagtcga cctggatgtc actacgcagc aaaaagagaa ggccatggcg
     2761 tggttcggca acggcatgaa gaagatcgcg gattggcccg tggcgcgaag cgaaaacatg
     2821 gacggcatca agctgtacct gggcgacgta ggctgggtga tggtgcgcgc gtcaggaacg
     2881 gagaatctgt tgcgcgtcta cgccgaaact tcgcgcagtg aaacgacacg aaaagtactg
     2941 agcgcggtta ccagcagaat tcaagaactc tagtcggagc ggcagccatg caaaccgtca
     3001 acatcgcgga gaagttgtca caatttgagg gaacgtggag tcccaagatt cttggcgaag
     3061 tgaatgatag ctatgtcaag gccgtgaagc tgaagggcga gtttgtctgg cac
//
LOCUS       DASTPM010000729         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1439615, whole genome shotgun sequence.
ACCESSION   DASTPM010000729 DASTPM010000000
VERSION     DASTPM010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439615"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1354)
                     /locus_tag="VFR42_12905"
     CDS             complement(149..1354)
                     /locus_tag="VFR42_12905"
                     /inference="COORDINATES: protein motif:HMM:NF025022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU0370108.1"
                     /translation="MNRTLPDPPPSQSQSTESSDPAPPVRNLADSASSSGFAREWNFW
                     LFRCFLVAVAAAVALSLSPFGLHGWYAAGAGLVLMFVIFIVEHHLRRASSPALLGGAI
                     GGILGVLAALLISSVIAHTAEPEPSKSFIEYVILLGFGYTGVMLGARKFGSLKTGSLN
                     ALFGLRPGAPSIHADGKLMDTSVLIDGRVADICEAHFLDGPLLVPQFVLHELQTIADS
                     PDSLRRQRGRRGLEILQRIQKLPSHEVRIIEDAPGQTGDVDRKLVELALRFQAKIVTN
                     DFNLNKVASVQGINVLNINLLANALRPAVLPGEGMRVFSLREGKEVNQGVAYLEDGSM
                     VVVDGARRLINKTVDILVTSVHQTPAGKMIFGRLDDRHDTPPPLRQSVVGARSDRSPR
                     PLYPETNSE"
     gene            complement(1640..2845)
                     /gene="alr"
                     /locus_tag="VFR42_12910"
     CDS             complement(1640..2845)
                     /gene="alr"
                     /locus_tag="VFR42_12910"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0370109.1"
                     /translation="MTTKKKSATANGRPVWAEVSLSALTHNLRAIRDFVNPKSEKRPA
                     PRKVLCIVKGNGYGHGGPQVAKHLEKVGADWFGVTGAAEGEAVRRAGVAKPILALTSF
                     WPGEEDQLLKNKIAPAVIRCEQLAALESAAARRKLAKRSVGIQLKIDTGMNRLGIAPT
                     DIDCFARQYEKCSRLILQGVFTHLASSEVFAGPAAQQTCDQESSFHRAVERLRTLGID
                     PGLIHLANSAAIASRPETWADMVRPGAILYGYHPGYDPIERRVEAEKQLPLRPVMSLC
                     TRIINVRHVSAGEGVGYNAKFVASRPSKIAVLAAGYGDGIHRSLGNKGHVLVNGVLAP
                     IVGIISMDVTMADVTDVPDVALGDVATVYGFDGERLLAANLVARSIGTVTSDLLCAVS
                     ARVPRLYVS"
BASE COUNT          676 a          748 c         1004 g          561 t
ORIGIN      
        1 agagcgacag gcgatacgcg caaggaacac gaaaaacgtt agcacagcag ttactaaata
       61 tcaacagtac ttgatctaaa aatcgatttg gtcaaggttg agtttcagaa tgggaattct
      121 tcgattcgat agggcggaga aattcttctt attcactgtt agtttcggga tagagaggac
      181 ggggagaacg gtcggagcgg gcgccgacga cggattgccg gagcggcggc ggagtatcgt
      241 ggcggtcgtc caagcggcca aagatcattt tcccagcggg cgtctggtgg accgaagtga
      301 cgagaatgtc cacagttttg ttgatgaggc ggcgcgcgcc gtccacgacc accatcgagc
      361 cgtcttcgag gtacgcgaca ccctgattga cttccttgcc ttcgcggaga ctgaagaccc
      421 gcatgccttc gcccggaaga acggccggac gaagggcgtt ggcgagcaga ttgatgttga
      481 gaacgttgat gccctggaca gaagcgacct tgttgagatt gaaatcgttg gtgacgattt
      541 tggcctggaa gcggagtgcc aattcgacga gcttgcgatc gacatcgcca gtttgcccgg
      601 gagcgtcctc gatgatgcga acttcgtggg atggcagctt ctgaatgcgt tgcaggattt
      661 caaggccacg ccggccacgc tggcggcgca ggctgtcggg tgagtcggcg atagtctgta
      721 gttcgtgcaa gacgaattgg ggtaccagca gggggccatc gagaaagtgg gcttcgcaga
      781 tgtcggcaac acggccgtcg atgaggacgc tggtgtccat gagcttgccg tcagcgtgga
      841 tggaaggggc gccagggcga aggccaaaaa gggcattcag cgagccggtc ttgagtgagc
      901 caaacttgcg cgcgccgagc atgacgccgg tatagccgaa accgagcaaa atgacgtatt
      961 cgatgaagga tttgctgggt tcaggttcag cggtatgagc gatgacggag ctgatcagca
     1021 acgcagcgag cactcccaga atgccgccaa tggcgccacc caggagagcg ggggaggaag
     1081 cacgcctgag gtgatgctcg acaatgaaga tgacgaacat caggacaagt cctgctccag
     1141 cagcatacca accatgaagg ccaaagggcg aaagactgag agccaccgca gcggcaacag
     1201 cgacgagaaa acaccggaag agccaaaaat tccattcccg cgcaaaaccg gaggaactgg
     1261 cactatcggc caagttccgc acggggggag cgggatcgga agattccgta gattgagatt
     1321 gagacggcgg cggatcgggc aaagtgcggt tcatgcgacc tttcagcgcg ggttgctgaa
     1381 actcagcatt acggactcaa aaacgcgcgc agacccggca cctctggtgg tgcaacactg
     1441 cacaaatcgt accgcgattc tgcccaggag gacgacctcg gcagaatctc aacagcctgg
     1501 catgcccctt ggtacctata tacgcctggg accggttggg cgtcaatggc agcgaacgtg
     1561 actaatggcg gtgattggtg attggtggcc gctgcgcttc aactagcgcc caagcgaggc
     1621 cgcccgtgtt aggagaagtt caggaaacat aaagccgcgg aacgcgggcg ctgacagcgc
     1681 agaggagatc ggaggtgacg gtgccgatgc tgcgcgcgac gagattggcg gcgagaagtc
     1741 gctcgccgtc aaagccgtag accgtggcga catctccaag agcgacgtcc ggtacatcgg
     1801 tgacgtcagc catggtgacg tccatggaaa tgataccgac gatgggagcg aggacaccgt
     1861 tgaccagaac gtggcctttg ttgccgagcg agcgatgaat gccgtcgccg tagccggctg
     1921 cgagaaccgc aattttggat ggacggctgg cgacgaattt tgcgttgtag ccgacgccct
     1981 cgcccgcgga tacgtggcga acgttgatga tgcgggtgca caggctcatc acgggccgca
     2041 gtggcagctg tttttcggct tcgacgcggc gttcgatggg atcgtagccg ggatgatagc
     2101 cgtacagaat tgcgccgggg cgaaccatgt cggcccaagt ttcgggacgg gaggcgatgg
     2161 cggcactgtt ggcaaggtga atcaggccgg gatcaattcc gagagtgcgg agacgctcta
     2221 cggcgcggtg aaaactggat tcctggtcac aggtctgctg agcggccgga ccagcgaaaa
     2281 cttcggaaga agcgaggtgt gtgaagacgc cctggagtat caagcgagag catttttcgt
     2341 actgacgcgc aaagcagtcg atgtccgtag gcgcgatgcc gaggcggttc atgccagtgt
     2401 caattttcag ctggataccg acggagcgtt tggcgagctt gcgtcgcgcc gcagcggatt
     2461 ctagagcggc aagctgttcg caacggatga cggcaggtgc gattttattt ttgaggagct
     2521 ggtcttcttc accgggccag aaactggtga gcgcgagtat cggtttggcg acgccagctc
     2581 tgcgtacagc ttcgccctcg gcagcgccgg taacgccgaa ccagtcggcg cccacttttt
     2641 ctagatgctt ggcaacttgt gggccgccgt ggccgtatcc gttgcctttg acgatgcaaa
     2701 ggaccttgcg cggggccggg cgcttttcgg atttgggatt tacgaaatcg cgaatggcgc
     2761 ggagattgtg ggtgagtgca gagagagaga cttcggccca gacggggcgg ccgtttgcag
     2821 tggcggactt tttcttagtg gtcatcgctc ttccaaagaa cttctatttt agaacatcgg
     2881 cgcgcacgcg ctcgaatcac ggggaacgtg agacgagaaa ggcaacaggc caaattgcac
     2941 ggggagtgcc agcggaggag agacgttggg cgttgaaacg gaagaggtt
//
LOCUS       DASTPM010000730         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_603363, whole genome shotgun sequence.
ACCESSION   DASTPM010000730 DASTPM010000000
VERSION     DASTPM010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603363"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFR42_12915"
     CDS             <1..922
                     /locus_tag="VFR42_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU0370110.1"
                     /translation="PFEQLPQALNTESGLIATANARVVGPAYKPYLTDHWEEPYRTAR
                     IWDLLHDKHDLRPADMMKVQADTYSYPDIFLAEQLAPATKIASPKDPRVQKLIALSKD
                     WNGIADSDSIVVPFLEATRRAALNLVLQPVLGDDTNVYRWRSTAFLQRVLTERPPSWL
                     PSAYKNYDELLVAAADLAVQQLQRDTGSSKIEDWSWKRFDSLDMFHPLGREGWLKRLF
                     SITGKPQSGTAFSPRAATRHHGPAMRFVANPGNWDDSLMLIPGGQSGQLGSSHYSDQF
                     SYWYEGTPIVAPFSDSAQSATRKHTLTLKP"
     gene            1086..1361
                     /locus_tag="VFR42_12920"
     CDS             1086..1361
                     /locus_tag="VFR42_12920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0370111.1"
                     /translation="MNTPVPKKSDPPKKPDFERCLSRLEEVVRKLESPELSLDDAMKL
                     FEEGVALSAECQKQLEEAEGKVEILLKKADGRVVAEPFEPEGEPEST"
     gene            1399..2280
                     /locus_tag="VFR42_12925"
     CDS             1399..2280
                     /locus_tag="VFR42_12925"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU0370112.1"
                     /translation="MTLPDFFEQDLQVVDAALERLLPAAGTPPPSIHEAMRYSVFAGG
                     KRIRPILCLETARIFSPDITPALHPGCAIEFIHTYSLIHDDLPALDNDDLRRGKPTCH
                     KKFGEAIAILAGDALLTRAFETIGATPVAPDLRVKILTEVASCAGTINGMVGGQVADL
                     EAEGKGVGPETLEYIHRSKTAALICASVTAGAICAGAPDEDVKRLRHFGETIGWAFQV
                     TDDILDVEESSAALGKTAGKDIAQQKATYPSVFGLERSHQIANDLASQAIADLAPYAD
                     RAARLRQIANFLIHRRA"
     gene            2287..3030
                     /locus_tag="VFR42_12930"
     CDS             2287..3030
                     /locus_tag="VFR42_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU0370113.1"
                     /translation="MTRSPKLRLDKVIVERGLAHSREKAQAMILAGEVLVNGVTAVKA
                     GHSVSDDAQIEIRSRLQKYVSRGGFKLEGALQDFSVDPTGTICLDLGSSNGGFTDCLL
                     QHGAAKVFAVDVNTDQLDWKLQQDSRVLRIKRNARELQLADLSEPAALVVADVSFISV
                     TKILPAAVSCAAPGADFLILIKPQFELRREDIGPGGIVSDPDLHQRAIASVQCAAESL
                     ALQVFGIKPSHLPGAEGNQEFFLHTRRPK"
BASE COUNT          692 a         1116 c          737 g          645 t
ORIGIN      
        1 tcccttcgag cagttgcccc aggctctcaa tactgaaagc ggactcatcg ctacagcgaa
       61 cgcgcgcgtc gtcggcccgg cgtacaaacc gtatctcacc gatcattggg aagagcccta
      121 ccgcaccgcg cgcatctggg atcttctgca cgacaaacac gatctccgtc ccgcggacat
      181 gatgaaagtt caggccgaca cctatagcta tcccgatatt ttcctcgccg aacaactcgc
      241 gcccgccaca aaaattgctt cgcccaaaga cccgcgcgtg caaaaactca tcgctctctc
      301 caaagattgg aacggtatcg ccgactccga ctccatcgtc gttccatttc tcgaagccac
      361 tcgccgcgcc gctctcaatc tcgtccttca gcccgttcta ggcgatgaca caaatgtcta
      421 ccgatggcgc agcaccgcgt ttcttcagcg cgtcctcacc gaacgcccgc cgtcatggct
      481 accttccgcc tacaagaatt acgacgaact cctcgtcgct gccgccgacc tggccgtgca
      541 gcaactccag cgggacaccg gctcatcaaa aatcgaagac tggtcgtgga agcgtttcga
      601 ttcgctcgat atgtttcacc cgctcggccg cgaaggctgg ctcaaaagac ttttcagcat
      661 caccggcaaa ccgcaaagcg gcaccgcttt cagcccacgc gctgccactc gccatcacgg
      721 tccggcaatg cgtttcgtcg ccaatcccgg caactgggat gattccctca tgctcattcc
      781 cggcggccaa agcggccagc tcggcagcag ccactactcc gaccagttca gctattggta
      841 cgaaggcact cccatcgtcg ccccgttctc cgactcagct caatccgcga cccgcaaaca
      901 caccctcact ctcaaaccgt agccgtcttt gtagcggcca ggtttaggcg gcctcctaga
      961 ttgcgacgcc aggtttcatt tcaccttgct gagcgaaagc caatctccgg acgctcacgg
     1021 cgctcacggc ttccaacttg tttcctctct ccgccctgcc gtaaactgtt ccctgcgaaa
     1081 aacacgtgaa cacacctgta cccaaaaaat ccgatcctcc caagaaaccc gacttcgaac
     1141 gctgcctctc gcgcctcgaa gaagtcgtcc gcaaacttga aagcccggaa ctctctctcg
     1201 acgacgccat gaaacttttt gaagaaggag tcgccctttc cgcggaatgc cagaagcaac
     1261 tcgaagaagc agaaggcaaa gtcgaaatcc ttctcaagaa agccgacggc cgagtcgtcg
     1321 ccgaaccttt cgaacccgaa ggcgagcctg aatccacgtg atcacgggac aatgtctgcc
     1381 tctccacaac attcctcaat gactcttccg gacttcttcg aacaagactt gcaagtcgtg
     1441 gacgcggccc tcgaacgtct tctcccagcc gccggcactc ctcctccatc cattcacgaa
     1501 gccatgcgct actctgtttt tgctggcggc aaacgcatcc gtcccattct atgtcttgaa
     1561 accgcccgca ttttcagccc agacatcacc cccgctctcc atcccggctg cgctatcgaa
     1621 ttcatccaca cctactcgct catccatgac gatcttcccg cgctagataa cgacgacctg
     1681 cgccgcggca agcccacgtg tcacaaaaaa ttcggtgaag ccatcgctat cctcgccggc
     1741 gacgcgcttc tcacgcgcgc tttcgaaact atcggcgcaa ctcccgtcgc tcctgatctt
     1801 cgcgtcaaaa ttctcaccga agtcgcctct tgcgcgggca ccattaacgg catggtagga
     1861 ggccaggtcg ccgatctcga agccgaaggc aaaggcgtag gccccgaaac gctcgaatac
     1921 attcatcgca gcaaaaccgc agcactcatc tgcgcgtccg tcactgctgg tgcgatttgt
     1981 gccggcgcgc ccgacgaaga cgtaaaacgc ctccgccatt tcggcgaaac tatcggctgg
     2041 gcattccagg tcactgatga cattctcgac gtcgaggaat cttccgccgc gctcggcaaa
     2101 accgccggca aagacatcgc ccaacaaaag gccacttacc cgtccgtctt tggcctggaa
     2161 cgctcccacc aaatcgctaa cgacctggca tcgcaagcca tcgccgacct tgctccctac
     2221 gcggaccgcg ccgcccgcct ccgccagatc gccaactttc taatccaccg acgcgcctga
     2281 ccagggatga cgcggtctcc aaaactgcgc ttggacaaag ttatcgtgga acgaggtctg
     2341 gcgcattctc gcgaaaaagc tcaagccatg attttggctg gagaagtttt ggtcaacggc
     2401 gtgaccgctg tcaaagctgg ccattccgtt tccgacgacg cgcaaattga aatccgcagc
     2461 cgcctgcaaa agtatgtttc gcgcggcgga ttcaaactgg aaggcgctct gcaagatttc
     2521 tcagtggacc caacaggaac gatttgcctc gacctcggct cctccaatgg tggcttcacc
     2581 gactgccttc tgcaacacgg cgccgccaaa gtcttcgccg ttgacgtcaa caccgatcag
     2641 ctggactgga aactccagca agactcgcgt gtcctccgca tcaagcgcaa tgcccgcgaa
     2701 ctgcagctcg ccgacctctc cgaacccgca gcgctcgtcg tggccgacgt ctccttcatc
     2761 tccgtcacca aaattcttcc cgccgccgtt tcctgcgccg cccccggcgc cgattttctc
     2821 atcctcatca aaccccagtt tgaactccgc cgcgaagaca tcggccccgg cggcatcgtc
     2881 tctgatcctg accttcatca acgcgccatc gcctccgttc aatgcgccgc cgaatctctt
     2941 gccctccaag tttttggcat caaacccagc cacctgcccg gcgccgaggg aaatcaagag
     3001 tttttcctgc atactcgcag gccgaagtag ccttgctggg tgcattgtgc aggcacggtc
     3061 agatgtaagc gatgttgttt ctgtaagtta agaagcggca ccccgcccac cggtattttt
     3121 cgccaaagag ttcgcaagtc tttgaaaagc cgatgtgttt gaggcgctct gctttgcact
     3181 ggagtgcaca
//
LOCUS       DASTPM010000731         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1151011, whole genome shotgun sequence.
ACCESSION   DASTPM010000731 DASTPM010000000
VERSION     DASTPM010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151011"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFR42_12935"
     CDS             <1..301
                     /locus_tag="VFR42_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370114.1"
                     /translation="HVTEKGFKSSKSDKADKSADKSADKSADRSDEKSYERSRVDVKI
                     PMKVVEALLSAGKDELDLVAALRALSAHGGDTELVSVKSDDSTVRVWLDSKNVSD"
     gene            356..793
                     /locus_tag="VFR42_12940"
     CDS             356..793
                     /locus_tag="VFR42_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370115.1"
                     /translation="MMILAKLALGTASTLMIAGVYTFREGTIRVDVDEHREGGAHVHV
                     WAPAAAVPMALHFVPDDKIREATEHANEWAPLVRIAAKELRKYPNTTFVEVEDGQDHV
                     KVSTVGAKIRVDVTNPQENVHVTVPLAMVEDVIAQIAARQPAS"
     gene            complement(915..2231)
                     /gene="glyA"
                     /locus_tag="VFR42_12945"
     CDS             complement(915..2231)
                     /gene="glyA"
                     /locus_tag="VFR42_12945"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU0370116.1"
                     /translation="MTKTTEANSRMQRPLQEVDPQIAGLLHDEAEREATGLELIPSEN
                     LVSEAVLEAMGSVFTNKYAEGYPGKRYYGGCEFADKVEQLAIDRAKELFGAEHANVQA
                     HSGTQANVSVYMAALQPGDTVLGMNLSHGGHLTHGHPLNFSGRMYKFVAYGVRQDTEQ
                     IDYDEIEKLAAEHKPKMIVAGASAYSRVIDFERMAKAAKSVGALFFVDMAHIAGLVAA
                     GVHPSPVPHADFVSTTTHKTLRGPRGGMVMSKAAFQKELDKLTFPGLQGGPLIHTMAA
                     KAVCLKEAMEASFKEYQQQVAKNAKALAAAVAAKGLRVISGGTDNHLLLVEVHSRGIT
                     GSDAEKALDRAAITVNKNAIPFDPLPPMKAGGIRLGTPSITTRGMKEPEMALIAGWIA
                     EVLGAIGNAAAQAEVEQRVRKEVAALAAKFPIYEARVAKKAQSTTV"
BASE COUNT          513 a          780 c          733 g          525 t
ORIGIN      
        1 ccacgtcacc gaaaagggat tcaagagcag caagtccgac aaggctgaca agtcggctga
       61 caagtcggcc gacaagtcag cggacaggtc tgacgagaag tcctacgagc ggtcacgcgt
      121 cgacgtgaag attccgatga aggtagtgga agcgctgctg tctgcaggta aggatgaact
      181 ggatttggtg gcggcgctgc gcgcgctatc cgctcatggc ggcgacacgg aactcgtctc
      241 ggttaagagc gatgactcga cggtgcgcgt ctggctcgac tcgaaaaacg tttcggacta
      301 agcccgaaga aatcactgaa gaaatccagg gaaggaaaac actgaggaga cgcacatgat
      361 gatcctggct aaactcgctc tcggcaccgc ctccactctg atgatcgccg gtgtgtacac
      421 cttccgcgaa ggcaccatcc gcgtcgacgt tgacgagcac cgcgaaggcg gcgctcacgt
      481 ccacgtgtgg gcgccggcgg ccgcagtgcc catggcgctg cactttgtgc cggacgacaa
      541 gatccgcgaa gccaccgagc acgccaacga gtgggcgccg ctggtgcgca tcgccgcgaa
      601 agaactccgc aagtatccca acaccacgtt tgtggaagtc gaggatggcc aagaccatgt
      661 caaggtcagc acggtcggcg ccaagattcg ggtggacgtc accaatcccc aagaaaacgt
      721 ccacgtcacc gttccgctgg cgatggtgga agacgtgatc gcgcaaatcg cggctcggca
      781 gccagcctcg taaacggaaa agcgaaactc gaaattcggg tgccgcactc ttcgactttt
      841 agggcgacgt gttttcgagg tggcccgacc acgcccgatg caaatcgccg aacactccaa
      901 ctggactcct gcttttatac agttgtactt tgcgccttct ttgctacgcg cgcttcgtag
      961 atggggaact ttgcggctag cgctgctact tcttttcgca cgcgttgttc cacttcggcc
     1021 tgggcggctg cgttccctat tgcgcccagg acttcggcga tccagccggc gattaaggcc
     1081 atttccggtt ctttcattcc tcgggtggtg atggaaggag tgccgaggcg gatgcctcct
     1141 gctttcatcg gcggcagggg gtcgaacgga atggcgtttt tgttgacagt gatagcggcg
     1201 cggtcgaggg ctttttccgc gtcactgccg gtaataccgc gcgaatgcac ttccaccagg
     1261 agcaaatgat tgtcggtacc accggaaatc acgcgcaggc ccttcgcagc cacggcagcc
     1321 gccagcgcct tggcgttttt ggccacctgt tgctgatatt ccttgaacga agcctccatc
     1381 gcttccttca agcagactgc ttttgcggcc atggtgtgaa tcagcggacc gccctgcaat
     1441 cccggaaacg tcagcttgtc cagctctttc tggaacgccg ctttcgacat caccatgccg
     1501 ccgcgtggcc cgcgtagcgt cttgtgcgtc gtcgtgctaa caaaatcagc gtgcggcaca
     1561 ggactcggat gcactcccgc cgccaccagc cccgcgatgt gcgccatgtc cacaaaaaat
     1621 agcgcgccca cgctttttgc cgccttcgcc atgcgctcaa aatcaatcac ccgcgaatac
     1681 gcgctcgctc ccgcgacaat catttttggt ttgtgttccg ccgccagttt ttcgatttcg
     1741 tcgtagtcaa tctgctcggt gtcctgccgc actccatacg ccacgaactt gtacatgcgc
     1801 ccggaaaaat tcagcgggtg cccgtgcgtc aaatgtccgc catgcgagag gttcatgccc
     1861 aacacggtgt cgcccggctg cagcgccgcc atgtacacgc tcacgttggc ctgcgtcccc
     1921 gaatgcgcct gcacgttggc gtgttccgcg ccaaacaatt ccttcgcgcg gtcaatcgcc
     1981 agctgctcca ctttgtccgc gaattcgcat ccgccgtagt agcgcttgcc cggatagccc
     2041 tccgcgtact tgttggtgaa gaccgacccc atggcctcca gcaccgcttc gctcaccagg
     2101 ttttcgctgg gaatcagttc gaggccggta gcttcgcgtt ccgcttcgtc gtgcagaagc
     2161 cccgcaatct gcgggtccac ttcctggagg gggcgctgca ttctggaatt ggcttcggtg
     2221 gtcttggtca tcgggttgtc aggtcctcct aggagatgga ctggagaaca tgataccgcg
     2281 agtgcggggc gaagccccca agtcgaaatg agaaattcga aactcgcaaa gcgaagggcg
     2341 aaggacgaaa actcgaaaag cggaaagctt gagtaccctc tggtattttt tttgtatgtg
     2401 cggcaaccaa aggacggccc cgtcgcgttc ccatatgcgt ggcacgcggc caaagttccg
     2461 ataaccacga gtgcgctagc cacgatccgg cgatgcacct ccacgaacac ctcctgcgac
     2521 ttggcaacta aggacgactt ggcggtgttg g
//
LOCUS       DASTPM010000732         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_920530, whole genome shotgun sequence.
ACCESSION   DASTPM010000732 DASTPM010000000
VERSION     DASTPM010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920530"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VFR42_12950"
     CDS             complement(<1..398)
                     /locus_tag="VFR42_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370117.1"
                     /translation="MVLHRWSRHERYQGLRIVIPASAVRRFTFAFGAAFALFYVVARL
                     RGLALFTVYPAQNIVLLGTHRSRDFADPVLDFLAPEMYWYGWTASAAVCALVIGLIAG
                     LLPDRLNWFWAWCMWVIPPMAMAACVHLTIP"
     gene            complement(344..808)
                     /locus_tag="VFR42_12955"
     CDS             complement(344..808)
                     /locus_tag="VFR42_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370118.1"
                     /translation="MATTLLILHGLVAVALIGAITHQALAACAPPNAKPNSFFGRFRA
                     IPAAGFANAIVLLYLTSWLLGAVVYLYFKVDIQPTLERDRHWHAMGFLDLKEDFVVIG
                     LGVLPAYWFCWRGTDNSQHHHLRAALTVLLAFIVWWSFIVGHVMNDIRGFGS"
     gene            complement(839..2227)
                     /locus_tag="VFR42_12960"
     CDS             complement(839..2227)
                     /locus_tag="VFR42_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0370119.1"
                     /translation="MRRNVKLPLGELEKNATSHDAVSADRKSGLNRRALLSGGAVLPV
                     LPSFLFGNTRRAAAQQASSGFSFAIYGDSRPMMYLPYKDGRPDLVKLFVEMFGLVMPQ
                     RVAEAVVKRDVKMVFDPVTKELIRVVMPFMSRTEVMTLTVDQGWVTRATVEDVKLLPG
                     VHRDMFELAGSDWVSREIVQQVRSGRAKFVVNSGDVVWWGNQGLTINDSPYWKRLNDS
                     MLKLLPEPDAEMRAAGLEGRWLMSVGNHEVWGDPKIQGTLNAVPYLKNFGVTPERLIY
                     KFDFKDVRFIFLWSGKYDYRSPSLWDADRPKYAEQMVQLREWLDDAKAKGMKKAFITF
                     HYPVFCRAGLGPIPEPDNPHKVIAAYAKDLELVVFNGHVHTTELYDVDGVKYLVLGGG
                     GAEQDPILPGRTSIKVPAGYPPDVYWKGKPPQLEYNYVLVDVDPGNKTKFTLTRYRPG
                     SAEPFATEALFS"
BASE COUNT          572 a          730 c          667 g          480 t
ORIGIN      
        1 ggtattgtga gatggacgca tgcggccatt gccattgggg gaatgaccca catacaccat
       61 gcccagaacc agttcaggcg atctggcaaa aggccggcaa tgaggccgat cacgagagcg
      121 cagaccgcgg cagaagcagt ccatccgtac caatacattt cgggcgcgag gaaatccaaa
      181 acgggatcag caaaatcccg ggaacggtgc gtgcccaaaa gaacgatgtt ctgcgcgggg
      241 tagacggtaa acagcgcgag cccgcgcaac cgcgcaacga catagaagag cgcgaacgct
      301 gccccaaagg caaacgtgaa gcgacgaact gccgaagcgg gtatcacgat ccgaagcccc
      361 tgatatcgtt catgacgtga ccaacgatga aggaccacca gacaatgaag gctaggagta
      421 cggtcagcgc cgcgcgtagg tgatggtgtt ggctattgtc ggtcccacgc caacagaacc
      481 agtacgccgg cagtacaccc aagccgatga caacaaagtc ctccttgaga tcaaggaatc
      541 ccatcgcatg ccaatggcgg tcgcgttcta aagtcggctg aatatcgact ttgaaataca
      601 gatagacgac cgcgccgagc agccaggagg ttaaatacaa aaggacgatg gcattggcga
      661 aacctgcggc tgggattgct cggaatcgac cgaagaaaga attgggcttc gcgttgggtg
      721 gcgcacatgc cgccagagct tgatgcgtaa tcgcgccaat caaggcgacc gcgaccaagc
      781 cgtgcagaat tagaagcgtt gtcgccatgc aagacgagct tgttaccgac cggtcttcct
      841 atgagaatag cgcttctgtt gcgaacggct cagccgatcc tggccggtaa cgcgtcaggg
      901 tgaatttcgt cttattgccc ggatcgacat cgaccaaaac ataattgtat tccagctgcg
      961 gcggtttgcc cttccagtag acgtccggcg gatagccggc cggcaccttg atgctcgtgc
     1021 ggcccggaag gatcgggtcc tgctccgccc cgccgccacc aagaacaaga tatttcacgc
     1081 catcgacatc atagagctca gtcgtgtgaa cgtgcccgtt gaagacaacc aactccaggt
     1141 ccttggcgta ggccgctatc actttgtgag gattatcggg ctctgggatg gggccaaggc
     1201 cggcgcgaca gaagaccggg taatggaaag taataaacgc ctttttcatt cctttcgctt
     1261 tggcgtcgtc cagccattcc cgcaactgga ccatctgctc cgcgtatttt ggccgatccg
     1321 catcccacag cgacggtgaa cggtagtcgt acttcccgct ccaaaggaaa atgaaacgta
     1381 cgtccttgaa atcgaacttg tagatcaatc gttccggcgt tacgccgaag tttttcagat
     1441 agggcaccgc gttaagtgta ccctggatct tcggatcgcc ccacacttcg tgattaccga
     1501 cggacatgag ccagcgcccc tcgagcccgg cagcgcgcat ttcggcgtct ggctccggca
     1561 gcagcttgag catgctgtcg ttcagacgct tccagtacgg actatcattg atcgtcaggc
     1621 cctgattgcc ccaccacacc acgtcgccgc tgttgacgac gaatttggcc cttcccgacc
     1681 gcacctgctg cacgatctcg cgcgagaccc aatcgctacc agcaagctca aacatgtccc
     1741 ggtgcacccc cgggagcaat ttcacatctt cgacagtcgc gcgagtgacc caaccttggt
     1801 ccacggtcaa cgtcatgacc tcggtgcgtg acatgaacgg catcacgacg cgaattaatt
     1861 ctttggtgac gggatcgaag accatcttca catcgcgctt caccaccgcc tcggcgaccc
     1921 gctgcggcat caccaagcca aacatttcga cgaacagctt gaccaaatcc ggacggccat
     1981 ctttgtacgg caggtacatc atgggtcgcg agtcgccgta gatcgcgaac gaaaacccgg
     2041 agctggcttg ttgtgcggcg gcgcgccgcg tgtttccgaa cagaaacgac ggcaacaccg
     2101 ggagcaccgc tccgcctgaa agaagtgccc ttctgttcaa cccgctcttt cgatcggctg
     2161 ataccgcatc gtgggaagtg gcgttctttt ccaattcacc cagaggaagc ttcacgtttc
     2221 gacgcatgcg atgtctcccc gactggtgcg cccttggact gcttttgtca caacgaaagg
     2281 cgctcgaata ttgcaccttg gggaaatgcc atgtccgctt ttggcggccg gttcaaccgg
     2341 ttgacgcaac acttgcgtta aatctccccc aatgcggggc tacttggcac aaatggaaga
     2401 tgacggctta ctaaacgctc gcccgaccgc tttgagcgaa tccccctct
//
LOCUS       DASTPM010000733         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_459288, whole genome shotgun sequence.
ACCESSION   DASTPM010000733 DASTPM010000000
VERSION     DASTPM010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459288"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VFR42_12965"
     CDS             complement(<1..763)
                     /locus_tag="VFR42_12965"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HEU0370120.1"
                     /translation="MNAQGKIAAHPTGKVLILPGSFKSAIRLLVPREHGTWGLLLLPL
                     ISGAIMGYGASSNASLAAVLWFLLTTLSVFLMYQPLEGLLGLSLIKARSQQQQRIAIL
                     SVITLALIATAGVLELFHLHRELVLSFGLAALGCFCVRLLLGSSRSTRVPRQLIGALG
                     LSSAAAGAYYAASGRIDRTGILLWLASWLFAVGQIEYVHLRISTAQVRSWRQKVRLSV
                     AVCFLYLLITGTSLTLTFAGFAPLLLTLAFVPSIIR"
     gene            complement(868..1200)
                     /locus_tag="VFR42_12970"
     CDS             complement(868..1200)
                     /locus_tag="VFR42_12970"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0370121.1"
                     /translation="MSENKQHPWTLPRAHTRYLLDLRIVVRAKETLFGRTKDIAEGGL
                     GATIPNNLDIGEIVELELQLPEANEPLKIKAEVRYRHGFQYGFKYVQITHQQKEMIRR
                     TTRDLRLA"
     gene            complement(1244..2335)
                     /gene="queG"
                     /locus_tag="VFR42_12975"
     CDS             complement(1244..2335)
                     /gene="queG"
                     /locus_tag="VFR42_12975"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU0370122.1"
                     /translation="MIFAQEIAQTAMQAATEAGFDLAGIAPIRKEDMPELGAFIEWVD
                     AGRAGEMRYLESRTESGDLRRASATNAAPWVRSVIVCALNYSADKPYSVAANDPKRGW
                     ISRYAWGTKDYHDALLPRLRKVEAAIKQVASARNAEIETRSYVDTGPILERVYAKYAG
                     IGWIGKNTCIIHQKLGSWLFLGVILTSLELPADTAAADRCGSCTRCIDACPTHAIVAP
                     GKLDARLCIAYLTIEKRGSVPEELRADMGHHIFGCDICQDVCPWNNKSGNAPPTSLPE
                     FQPQERLFHPDLRWLAQMDEETYRQVFRGSPVKRTKYSGLKRNVAIAMGNSGNKDFLA
                     DLQSLAAEPDAVVAEHAEWALRKLADKSR"
BASE COUNT          831 a          986 c          947 g          755 t
ORIGIN      
        1 cacggatgat cgacggaacg aaggcaagcg tcaacaggag cggcgcaaat cctgcaaagg
       61 taagagtaag ggacgtgcca gttatcagta agtacagaaa gcacacggcc acgctcagcc
      121 ggactttttg ccgccatgac ctgacctgcg cagtgctaat acgcagatgc acgtattcga
      181 tctgaccaac cgcaaaaagc catgacgcaa gccacaggag aattcctgtc cggtcaatcc
      241 tgccgctggc ggcgtaataa gctccggccg ctgccgaact aagtcccaga gcgccgatca
      301 gctgcctggg aactcgcgtg gatcgcgaac tgccgagcag caggcgcacg caaaaacatc
      361 ccagtgccgc caagccaaag ctcaggacga gctcacggtg caaatggaaa agctccaaaa
      421 ctccggctgt ggcgattaga gcgagcgtta ttaccgataa tattgcaatg cgctgctgct
      481 gctgcgaacg cgcttttatc agggacaatc ccaaaagacc ttccaaaggt tgatacatca
      541 ggaatactga taacgtagtc aataagaacc agagcaccgc ggccaacgat gcatttgaac
      601 tagcgccata tcccatgatt gcgccgctga tgagtggaag cagcaaaagc ccccaggtgc
      661 cgtgttctct gggaaccagc agtcgaattg cggacttgaa tgagccaggt aatatcagga
      721 cctttcctgt tggatgggcc gcaattttac cctgagcatt catcacaaac tattctcagg
      781 gagagggctt acattcggct gtgatgttga tcacagtaag aatggccgca cggcggaacg
      841 cgtattaagt actccgcgta tcgatgatta agccaggcgc aaatctcgtg tggtgcgccg
      901 gatcatctct ttctgctgat gcgtgatctg aacatacttg aatccgtatt ggaagccatg
      961 ccgatagcgg acttcagcct taatcttcag tggttcattg gcttccggga gctgcaattc
     1021 cagttctaca atttctccaa tatcaaggtt gttgggaatg gtagcgccca acccaccctc
     1081 ggcgatgtcc ttggtccggc cgaagagtgt ttcttttgcc cggacaacga ttcgcaaatc
     1141 caaaagataa cgggtgtgtg ctcgaggaag ggtccatgga tgctgtttgt tctctgacat
     1201 agctcctgaa aaccttaaca caatgttcct ttttgggcct tacttagcgc gatttgtctg
     1261 caagttttct gagcgcccac tccgcgtgct ctgcgactac agcatccggt tcagccgcca
     1321 aactctgtaa atccgccagg aaatccttat tcccgctgtt acccatggcg atcgccacat
     1381 ttcgcttaag gccagaatat tttgtccgct tcacgggcga gcctcgaaac acctggcggt
     1441 aggtttcttc atccatctgt gcaagccagc gcaaatcagg atggaaaagc cgctcctgcg
     1501 gctgaaattc cggcagggaa gtcggtggcg cgttgccgga cttgttgttc cacgggcaga
     1561 catcctggca aatgtcacag ccaaagatat gatgccccat gtccgccctg agttcttccg
     1621 gcacgctccc gcgtttttca atggtgagat aagcgatgca cagccgcgcg tccagcttgc
     1681 ccggcgccac aatggcatgc gttggacacg cgtcaatgca gcgggtgcaa cttccgcaac
     1741 ggtcggcagc tgcagtatct gccggcagtt ccagtgacgt gagaatcact cccagaaaga
     1801 gccatgagcc caatttctgg tggatgatgc atgtgttctt gccaatccat ccgatcccgg
     1861 cgtactttgc gtaaacgcgc tccagaatcg ggcctgtatc cacatagctg cgggtttcga
     1921 tttcagcgtt gcgcgcagag gcgacttgct tgatagccgc ttcaaccttt cgcagccgcg
     1981 ggagcagcgc atcatgatag tcctttgtgc cccaggcata gcgtgagatc cagcctcgtt
     2041 ttggatcatt ggccgccact gaataaggct tgtcagcgct gtaattcagc gcgcagacaa
     2101 tcacggaacg tacccagggc gccgcgttcg tggcagatgc acggcgaaga tcgcctgact
     2161 ccgtgcgaga ttccaggtac ctcatttctc cagcgcggcc tgcatccacc cattcaatga
     2221 aggcgccaag ctccggcata tcctctttgc ggatgggcgc tatacctgcc agatcgaagc
     2281 cagcctcagt ggcggcttgc atggcagtct gcgcgatttc ctgtgcaaat atcatcgtta
     2341 cctctgatta tccctcaaag cagctgcgcg gcagatgcct ctgtatcggg ccaaggtcag
     2401 ataacgtgac agatagtact aaggcccgtc tctctctgtg gtgctaagaa aaaattcacc
     2461 ttgcagctta tctgcgccag acatcatcct aataaataac tgagctgctg tttttagtag
     2521 acgtgtgtca aatagcgctc aaaggagttt aaagggcctc atgctttgta ccttctgcgg
     2581 gacagagaat cgtccggaat ataaattttg cgggtcttgc ggtgtaagac tggacagacg
     2641 gcacgccgaa cgaaggaccc accaagccgg aagcacaaag tgcgcgggat gcggccatgt
     2701 gaatgagccg ggcatgaagt tctgcggcat gtgcgggact cgcattgaac gccgcatgga
     2761 ggaacgtcgc ggtacgggtg acgcttcgcg ggccgcagcc attgccaacg cacaattgcc
     2821 acctccggat gtaaaacgca ggtcaacaac actggtggct gccccgacgg cggagccatc
     2881 tgttgaaacg gctgtagcgc ttcctcgcag aggcgaacca gcaatattcc gcaatgaacc
     2941 actgcgcaat ggacccgcac gcgccgaatc cgtgtccagt gaggcagttc gcaaagagtc
     3001 gggaaacggt ggtcgcatta gtggtccgtc ttttctgggt ttaaattctc agccagacaa
     3061 caacgatggg gctgaatatc tgctggaaga cgaaccttcc ggcggcggtt tgcgtaagct
     3121 gcttctgctg ttagtgctgg ctgccattct gggactgctt ttcgtgcaat ggcgatcaag
     3181 tttcaaagcc agtccgaaac cgccggttcc ggcaaaatca gatccatcaa ctccagctac
     3241 tgcaccgcag ggaagcaacc agccggcaga tggcatagga ccgactgttg cggagtcccc
     3301 taaagttgca gccagcggag ccagcgcgat tgcgaacggc gctgccgctg aattgaaagc
     3361 gccaaggacc gagcctacgg cgctggagac tacagcgcct agtccattgg ccactggctc
     3421 ggatgcaggc acggcttccg caaaatcctc aaccggaaac tccctgccta attcagacac
     3481 caaaggcgca gaagaggccc gtattcctga ctacaaacc
//
LOCUS       DASTPM010000734         4582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1237795, whole genome shotgun sequence.
ACCESSION   DASTPM010000734 DASTPM010000000
VERSION     DASTPM010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237795"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /gene="pdxS"
                     /locus_tag="VFR42_12980"
     CDS             complement(<1..700)
                     /gene="pdxS"
                     /locus_tag="VFR42_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU0370123.1"
                     /translation="MLKGGVIMDVTNAEQARIAEKAGACAVMALERVPSDIRKEGGVA
                     RMASIKVIREIMETVTIPVMAKVRIGHFAEAEVLQALEVDFIDESEVLTPADEENHIW
                     KHDYRVPFVCGARNLGEAVRRIAEGAAMIRTKGEAGSGNVVEAVRHMRTIVSEMKKLT
                     TLRDDEMMRAAKEIGAPLDLIRQIAKTGKLPVPNFSAGGIATPADAVLVRRLGAEAVF
                     VGSGIFKSSDPEARA"
     gene            complement(816..1250)
                     /locus_tag="VFR42_12985"
     CDS             complement(816..1250)
                     /locus_tag="VFR42_12985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0370124.1"
                     /translation="MTEKLILAPNPANNCFGCGGANDFGMKLTFELDPQTGRATGKFV
                     LGSRYAGGAGFAHGGVIAVLLDEVMGKISKLSDERAVTAELNIEYRKPVPTEAEIVVS
                     GWQEEEKGRNRFRVGEIHDSQGSLLARGRGRFVVVGQPVAAT"
     gene            complement(1270..2823)
                     /locus_tag="VFR42_12990"
     CDS             complement(1270..2823)
                     /locus_tag="VFR42_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0370125.1"
                     /translation="MLPVHLQPESHVPLYIQLRDQLRALVHAGDLRPGDRIPASRELA
                     TTLGVHRTTVANAYAELEAEGLIQGHVGRGTFIQGNGNGHALRFTPPPPSLGGNGDGG
                     LRWELLFADERGDEILSRLTASAPEDSLSFVMARPAADFFPVEELQICVNTVLRREAT
                     DVLNLGASDGYAPLKEALLELLHQDGIAAKDENLLVTDGCQQALDLISKAFVRPGDCV
                     VMENPTYPGAVAIFNGARARCIGVPVNTHPEPGKPMGVDLEAMEATLAANRVKLIVLT
                     PDFHNPTGTSMPLASRRKLLELATRHQVPVVEDHIYARLHARDERVPSLKQLDRSNLV
                     IHIDSFAKVAFPGLRVGWIVAAPAAIERLRQVKQTTDLHTDQLAQATLAEFLRRGYFS
                     KHLAKMRKVYALRLSALDEALRKNMPEETRWTRPEGGMCLWLELPPGFDASELLIHAK
                     ERGLLFAPGRYFYVQSHLPNTLRLGFANLDEKELTRGVATLADLLRVEMKKRQRGVRR
                     AERSRVALV"
     gene            complement(2935..4368)
                     /locus_tag="VFR42_12995"
     CDS             complement(2935..4368)
                     /locus_tag="VFR42_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370126.1"
                     /translation="MPCFWHTHVQAGDFGSHVYNAWLAQLAQRHQVSGIVVVKQWNNV
                     LFDLLMLHAANVFGFVAAERIVVSLGVLLFFWGAFSFLAVVARRRPWRVAPFLFVLAY
                     GYAFHMGFMNYYFSIGLALFALAAGLSGGAGNWLVAAGIAAFALVAHPLGFVLFVSLA
                     IYVSVWQRLPRWPRLALPLLAVGSFVLLRAYFHAHQNLVPSWRAEGLLQLLGQDQMNV
                     YGRRYLLLSWAALAWGGGSVIGAVYDWIFRGREPARALWLATEVYLMAVAATVCLPEN
                     FRTGLYAGWVGLLVSRLTLVSGVFGLLVLTSLRLPRWTWRGAAVIAAIYFGFLWQDTE
                     KLDRLEANARTLTQKLAPGTRIVAVANAPEDWRVPFIYHSIDRACIGHCFSFANYEAS
                     SLQFRVRALSGNSVVTTSVDQSDDMSSGDYRVRKRDLPLVSIYQCDDADFTRLCALPL
                     REGQKTEDPESEPVLNPAPDQDSDDEN"
BASE COUNT          963 a         1555 c         1182 g          882 t
ORIGIN      
        1 tcgcccgcgc ttccggatcg ctggatttga agattcccga gcccacgaac accgcctctg
       61 cgccgagacg ccgcaccagg accgcatccg ccggcgtagc aatcccacct gctgaaaaat
      121 tgggcaccgg cagttttccg gtcttggcaa tctggcgaat caaatccagc ggcgctccaa
      181 tttctttcgc cgcacgcatc atttcgtcgt cgcgcagcgt cgtcagcttc ttcatttcgc
      241 tcacgatcgt gcgcatgtgc cgaactgctt ccaccacgtt gcccgacccg gcttctcctt
      301 tggtgcgaat catcgccgcg ccttcagcga tgcgccgcac cgcttcgccc aaattgcgcg
      361 cgccgcacac gaacggcacc cggtaatcgt gtttccagat atggttttct tcatcggccg
      421 gcgtcagcac ttcgctttcg tcaatgaagt ccacttcgag cgcctggagc acctcggctt
      481 ccgcaaagtg tccgatgcgc acttttgcca tcacgggaat cgtcactgtc tccatgattt
      541 cgcgaatcac tttgatcgaa gccatccgcg ccacgccgcc ttccttacga atgtccgaag
      601 gcacacgctc cagcgccatt accgcgcacg cgccggcttt ctccgcaatt ctcgcctgct
      661 cggcgttggt cacatccata atcacgccgc ccttgagcat ctcggccaga ccgaccttca
      721 cacgccattc attcccagga ttgccagccg cgccattccc gcttccgttt actttcttcg
      781 tgtccatgtt cctctcctca aaaaacagaa tcttgttaag tcgctgccac cggctgcccg
      841 accaccacaa atcgcccgcg tccgcgcgcc agcaaactcc cctgcgaatc gtgaatctcg
      901 ccaacgcgaa accggttacg gcccttttcc tcttcctgcc agccgctcac cacgatttcg
      961 gcctcggtcg gtaccggttt gcggtactcg atgtttagtt ccgccgtcac cgctcgctca
     1021 tccgacaatt tcgaaatctt gcccatcact tcgtccagca gcaccgcgat cactccgccg
     1081 tgcgcaaatc ccgccccacc cgcataccgc gatcccagaa caaacttgcc ggtcgctcgc
     1141 cccgtctgcg gatccagctc aaacgtcaat ttcatcccga aatcattggc gccaccacaa
     1201 ccaaagcagt tgttcgcggg gttcggcgcc aggatgagtt tttccgtcat ttctcttcat
     1261 tttctctctt caaaccagtg ctacgcggct gcgttccgcg cgtcgcacac cgcgctgccg
     1321 ctttttcatc tcgacgcgca gcaaatccgc cagcgttgcc acgcctcggg tcagttcttt
     1381 ttcgtccaga ttcgcaaacc ccagccgcaa cgtattcggc aggtgcgact gcacgtagaa
     1441 ataacgccct ggcgcgaaca acaatccgcg ttccttcgcg tgaatcagca attcgctggc
     1501 gtcgaatcct ggcggcagtt cgagccacag acacattcca ccttcgggcc gcgtccaccg
     1561 cgtctcctcc ggcatgttct tgcgcaacgc ttcgtccagt gcactcaagc gcaacgcata
     1621 aaccttgcgc atcttcgcca aatgcttgga gaaataaccg cgccgcaaaa attccgccag
     1681 cgtcgcttgc gccagttggt ccgtatgcag atcggtcgtt tgtttcacct gccgcaaccg
     1741 ttcgatcgct gccggagccg cgacaatcca gcccacccgc aagccgggga acgccacctt
     1801 ggcaaaacta tcgatgtgga tcaccaggtt cgaccggtcc aattgcttca gcgaaggcac
     1861 ccgctcgtct cgcgcgtgca gccgcgcgta aatgtgatct tccaccaccg gtacctgatg
     1921 ccgcgtggcc agttccagga gtttccgccg cgaagccagc ggcatcgacg tcccggtagg
     1981 attgtgaaaa tccggcgtca acacaatcag cttgacgcga ttcgccgcca gcgtcgcttc
     2041 catcgcttcc aggtccacgc ccatgggctt cccgggctca ggatgcgtat tcaccggaac
     2101 gccgatgcag cgtgcccgcg cgccattgaa gatcgccacc gcgccgggat aggtcgggtt
     2161 ttccattacc acgcagtcgc ccggacgcac aaacgccttg ctgatcaagt ccagcgcctg
     2221 ctggcagcca tcggttacaa gcaaattttc atccttggcc gcaattccgt cctggtgcag
     2281 caactccagc agtgcttcct tcagcggagc gtagccatcc gacgcgccaa ggttcagcac
     2341 atcggtggcc tctcgccgca gcaccgtgtt cacgcaaatc tgcagttctt ccaccgggaa
     2401 aaagtccgct gcggggcgcg ccatcacaaa ggaaagcgag tcttccggcg cgctggccgt
     2461 cagccggctc agaatttcgt ccccgcgctc gtccgcgaaa agaagctccc agcgcaaccc
     2521 gccatctccg ttgccgccaa gcgatggcgg aggcggcgta aagcgcagcg cgtgaccatt
     2581 gccgttgcct tgaataaatg tgccgcgtcc cacatggccc tggatcagcc cctcggcttc
     2641 gagttcggca taggcgttgg ccacagtggt cctgtggacg cccaaagtgg tcgcaagttc
     2701 gcgactggcc ggaatgcgat cgccggggcg caagtcgcct gcatgaacca gcgcgcgaag
     2761 ctggtcacgc agttggatat acagcggaac atggctttca ggctgaaggt gtacaggaag
     2821 cattgtctct caccaggagc caattctatc agacattgga ctgatgcaag agccattttg
     2881 tctttttaca gaaatcagcc gccgattccc ggcaaaagat gctgcccgcg tcggtcaatt
     2941 ctcgtcgtca gagtcctggt cgggagcagg attaagcacc ggttcggatt cagggtcttc
     3001 ggtcttctgc ccttcccgca ggggcagggc gcacagcctg gtgaagtcgg catcgtcgca
     3061 ctgatagatg gaaaccagtg gcaggtccct tttgcgaacg cggtagtcgc cgctcgacat
     3121 gtcatcggac tgatctaccg acgtggtcac aaccgaattt cccgacaaag cccggacgcg
     3181 aaactgcagc gacgacgcct catagttggc aaaactgaag cagtgaccga tgcaggcacg
     3241 gtcgatcgag tgatagatga aggggacgcg ccaatcctcc ggcgcgttgg ctacggctac
     3301 aattcttgtt ccgggcgcca gcttttgcgt gagtgtgcgc gcgttcgcct ccagccggtc
     3361 aagcttttcg gtgtcctgcc ataaaaagcc gaagtagatc gctgcgatca ccgccgcgcc
     3421 gcgccatgtc catcgcggca ggcgaagcga cgtcagcacc aacaaaccaa acacgccgga
     3481 caccaaggtt agccgggaga cgagcagccc cacccagccg gcataaagtc ccgtccgaaa
     3541 gttttccggc aggcaaaccg tagcggccac cgccatcaga tagacttctg tggcaagcca
     3601 gagcgctcgc gccggctctc ttccacgaaa aatccagtcg tacacagcgc cgatcacgct
     3661 gccccctccc cacgccagcg cagcccatga caacagcaga taacgcctgc catacacatt
     3721 catctggtct tgtccgagca gctgcaataa accttcggcg cgccagctcg gcactaaatt
     3781 ctgatgtgcg tgaaagtagg cacggagcag cacgaatgaa ccaactgcga gcagaggaag
     3841 cgccaggcgc ggccagcgcg gcagcctttg ccaaacggag acatagatcg ccagcgaaac
     3901 aaacaatacg aaaccgagtg gatgcgcaac cagcgcgaac gcagcgatcc ctgccgcgac
     3961 cagccagttc cctgcccctc cgctcagccc ggccgcaagc gcaaacagcg ccagcccaat
     4021 cgagaagtaa taattcatga agcccatatg gaacgcatag ccgtaggcca acacaaaaag
     4081 aaatggcgcc acgcgccacg gcctccgcct tgcaacaacc gcaaggaagc tgaaagctcc
     4141 ccaaaaaaac agcagtaccc ccagcgaaac gacgattctc tcagccgcga caaatccgaa
     4201 aacattcgct gcatgcagca tcagcagatc gaacagcacg ttgttccact gcttcaccac
     4261 gactatcccg gagacttggt gccgttgcgc cagctgcgcc agccacgcgt tataaacgtg
     4321 gctgccaaag tcgcctgcct gaacgtgcgt gtgccagaag catggcacga gcagaaggag
     4381 ggagaccgca attaccgcaa gcgggttccc ccggacgaac cgcaaggcag cgcccagcgc
     4441 aaactcaggc gaattgctgg aaacacgaat tggccctgcg ctggttggcg gcatagtcag
     4501 gtcgccatct taatcgggcc ttccggtttt tgagccgact ctcaactcgg tggcgcatag
     4561 acgcgtccaa tcggccgcgt ca
//
LOCUS       DASTPM010000735         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_805680, whole genome shotgun sequence.
ACCESSION   DASTPM010000735 DASTPM010000000
VERSION     DASTPM010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805680"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1489
                     /locus_tag="VFR42_13000"
     CDS             230..1489
                     /locus_tag="VFR42_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU0370127.1"
                     /translation="MRVLEKDGRLICESADGLSLAVFHLMQNVPGDVFQIEEATTRQQ
                     IKFARDSSGNVAGLNFGGSAFVRLPPRNFDPAASSSASSEPLRSPAPFPGSTPPRLPG
                     DLINRTGDFANQNETDVFLLLERAGHLILRDSAGAEHPVSPLAFTRKGDQTSYTYASN
                     DTDPWVFAFEQYKSEPVWRLFTQLGPYHRLPYSVPTGSPNVVRVKPNRPLAQIRNEAL
                     AAFPPEEKGPFRKSDLVELASLDRAIRLDIRYATTNDFLGVPVYTQARAFLQRPAAEA
                     LLRALRKLEPLGYGLRIHDGYRPWYVTKIFWDATPADGKIFVADPAQGSRHNRGCAVD
                     LTLFDLKTGEPVEMTGLYDEMSPRSFPDYPGGTTLERWHRDLLRWAMESEGFTIFESE
                     WWHFDYKDWREYAIGNVPFEKLDVAHK"
     gene            1503..>2479
                     /locus_tag="VFR42_13005"
     CDS             1503..>2479
                     /locus_tag="VFR42_13005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU0370128.1"
                     /translation="MSFPILHFRGVGQLQAALRARRENCFFPLALALLPLLLFLLTPS
                     GSHAQQNPALAAAIEKVMDRPEYKRANFGIEFYALDTGSVLYAHAAEKLFVPASTTKT
                     LTEGALLANLGKDYRFHTRIFRAGKIDGKGRLKGDLILVASGDPNLSNRIQPDNTLAF
                     VDEDHSYGGPALPGDPLTVIKLLAKQVRAKGIRRIDGRVLIDTSLFPDGPREGGTDVV
                     MSSIMVNDNVFDVAAAPGAKVGDPLNVTTSPKTGYAHFTIHATTTAAGTIPVFNDPQF
                     AINSDGSVNITLAGSLPLGIEPQTVSLGVPSPTVFAQWVLRESLAAEGI"
BASE COUNT          497 a          799 c          663 g          520 t
ORIGIN      
        1 cccggactcg gctatcatgt ccgccacgac ctcatcgaaa gatggaccgt tgagaaacag
       61 attttccgcg cctgagaatc attcccgctg gcacgcgggt atctccactt ttttcctcgc
      121 gcttgcgctg gctcggtctc cactctgccg agcacaggct tcaaacacca gcaccaacgc
      181 aacgcgcgat ttcgtcggca cttataaaga acaatcgagc gaaaagagaa tgcgcgtcct
      241 cgagaaagac gggcggctaa tctgtgaatc cgccgatggc ctttccctcg cagtgtttca
      301 cctgatgcag aatgttcctg gcgatgtttt tcagatcgaa gaagccacca ctcgccagca
      361 gatcaaattc gccagagaca gctctggcaa tgtcgccggc ctgaacttcg gcgggagcgc
      421 tttcgtccgt ctgccgccca ggaactttga tcccgcagcg tcatcaagcg cgtcgagcga
      481 accgctacgt tcccccgctc cgtttcctgg ctcaacgcct ccacgcttgc cgggtgatct
      541 gatcaacagg acaggcgatt tcgccaacca gaacgaaacc gatgtttttc tgttgctgga
      601 acgtgcgggc catctgattc tccgcgattc cgccggcgcc gagcatccgg tcagcccgct
      661 cgcgtttacc agaaagggcg accagacgtc ctacacgtat gccagcaacg atacggaccc
      721 ctgggtcttt gctttcgagc agtacaagtc cgagccggtt tggcggctct ttacacagct
      781 tggtccctat caccgacttc cctattcagt gcccaccggc agtcccaatg tcgtgcgtgt
      841 caaaccgaat cggcctctgg cccagatccg taacgaagcc ctcgccgctt ttccccccga
      901 agagaagggg cccttccgca aatccgacct cgtcgaactg gcctctctcg atcgcgccat
      961 ccgtctcgat atccgctatg cgaccacgaa cgatttcctc ggcgtgccgg tgtacacgca
     1021 ggcgcgggca tttctgcagc ggccggcggc tgaagcgctg cttcgcgcgc tgcgcaaact
     1081 cgagccgcta ggctatggcc tgcgcatcca cgatggctat cggccctggt atgtcaccaa
     1141 gattttctgg gacgcgacgc cggcggacgg aaaaatcttc gttgccgatc cggcgcaggg
     1201 ttcacgccac aaccgtggat gcgccgtcga tctcactctg tttgacctga aaaccggtga
     1261 acccgtagaa atgaccggcc tctacgacga aatgtcgccg cgttcctttc ccgattatcc
     1321 gggcggcact acgctcgagc gatggcaccg cgacctgctg cgctgggcaa tggaatccga
     1381 gggtttcacc atttttgagt cggagtggtg gcacttcgac tacaaggact ggcgcgagta
     1441 cgcgatcggc aacgtgccat tcgaaaagct cgatgtcgca cacaaatagt tcaggaggca
     1501 ggatgtcgtt tcccattttg cacttccgag gagtaggaca gctgcaggcg gcattgcgag
     1561 cgaggcgaga gaattgcttt ttccctttgg cgctggccct cttaccgctc ttgctgttct
     1621 tgctgacacc atcaggtagc cacgctcagc agaatccggc gctggctgcc gcgatagaaa
     1681 aggtgatgga ccgacccgaa tacaagcgcg cgaacttcgg catcgaattt tatgcgttgg
     1741 ataccggaag cgtgctctac gcgcacgcag cagaaaaact attcgttccc gcctccacaa
     1801 cgaaaacgct gaccgaaggc gctctgcttg cgaacctcgg caaggactac cgctttcaca
     1861 ccaggatttt tcgcgccggc aaaatcgacg gcaagggccg cctgaaaggt gatttgatcc
     1921 tggttgcgtc cggcgatccg aatctctcca accgcattca gccggacaac accctggcgt
     1981 tcgtggacga agatcattcc tacggtgggc ccgctctgcc cggtgatccg ctgaccgtaa
     2041 tcaagctgct cgcgaaacag gtccgcgcca aaggcatccg ccgcattgac ggccgcgttc
     2101 tgatcgacac ttcccttttt cccgatggtc cgcgcgaagg cggcaccgac gtggtcatgt
     2161 cctccatcat ggtcaacgac aacgtcttcg atgtcgcagc cgcgccgggc gccaaagtgg
     2221 gcgacccgct taacgtgacc acttcgccaa aaaccggcta tgctcatttc actattcatg
     2281 caacgaccac ggccgcgggc accatccccg ttttcaacga ccctcagttt gccatcaact
     2341 ccgatggttc tgtgaacatc acgttagccg gttcgcttcc tctggggatc gagccgcaaa
     2401 ccgtttcgct cggcgtccct tcaccgaccg ttttcgccca atgggtgctg cgtgaatcgc
     2461 tggccgcgga gggcatcga
//
LOCUS       DASTPM010000736         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_834022, whole genome shotgun sequence.
ACCESSION   DASTPM010000736 DASTPM010000000
VERSION     DASTPM010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834022"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFR42_13010"
     CDS             complement(<1..296)
                     /locus_tag="VFR42_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370129.1"
                     /translation="MVSPTRANHRSAASPLSANMLPANLVSRFNVTFWSIGSFALTFP
                     QAENRVEKIGISGLSHWTSTLIPELDRRQFLSRTLAAAGATWTVAHWPAVLAAA"
     gene            317..934
                     /locus_tag="VFR42_13015"
     CDS             317..934
                     /locus_tag="VFR42_13015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0370130.1"
                     /translation="MATLTGAIGTGSKWLLDKIVGAIAATGINPNVLTFLGLVVNFGA
                     AACFAVGKFFYGALIIFFAGFLDMLDGQVARRQNRVTAFGAFYDSTLDRYADMALYMG
                     LLVYYSVSGRTPYVVLAAVATAGSVMVSYARARAESLIPLCKVGFMERPERLVLLIIG
                     GVFNRMAPVLWVIAVVSTLTVIHRIVFTWQETRAGRTLPGIKLSL"
     gene            complement(964..1566)
                     /locus_tag="VFR42_13020"
     CDS             complement(964..1566)
                     /locus_tag="VFR42_13020"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0370131.1"
                     /translation="MRVARDAAFDPQKTCFFISNHVNLFDPFVLYCAIPQFVRGWELE
                     SHFEIPIYGWLMKRFGNVPVPVVRRPSDLKRMWRLTRDAINGGTSLIVFPEGKRTRDG
                     HVTEFQDGAFRVAQQLEIPIVPVSIVGSFKHHRTGHWMFWPAEIRVFLHASIDTAGLT
                     KEDVPELRNRVKETIEQPIDEFLAGLTEAQQQPAEQEVVD"
     gene            complement(1736..>2466)
                     /locus_tag="VFR42_13025"
     CDS             complement(1736..>2466)
                     /locus_tag="VFR42_13025"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU0370132.1"
                     /translation="YRNFLAVGGDGTAHEILNGIFSRNASPDRITLGFLPLGTGNSFL
                     RDFTRAGSEASIQALLENRKRPVDLIRLTHATGEVYSFNILSLGFTADVGAITNRLFK
                     PLGHLGYLLGVFVRVVQLKRRAFTLRCDNETVWDDRRSLFLTFNNSKYTGGTMLIAPQ
                     ADPSDGFIEFVRWGPIGRLGLLRMLPRLYDGSHIEHPLASRKSVRHVEFNIPAPVDVM
                     IDGEILTLKCKSLDILPAALDVYI"
BASE COUNT          541 a          593 c          830 g          502 t
ORIGIN      
        1 tcggcggcag cgagaaccgc cggccagtgg gcaacagtcc atgtggcgcc tgccgccgcc
       61 agggtgcgcg acaaaaattg ccttcgatcg agttcaggaa tcaatgtgga tgtccaatgt
      121 gaaagtccag aaattccgat tttctcgact ctattctctg cttgcgggaa ggtcaatgca
      181 aaagaaccga tagaccaaaa cgttacattg aatcgtgaaa ccaaattggc aggaagcata
      241 tttgcggata gcggcgaggc tgcgctacga tggttggcgc gtgtaggcga aaccacacgc
      301 gctctcccac cgaagaatgg cgactttaac cggcgcaatc ggaaccggca gcaagtggct
      361 gctggacaaa atcgtgggcg caattgcggc caccggaatc aatcccaacg tattgacgtt
      421 tcttggactg gtggtgaatt ttggcgctgc agcgtgcttc gccgtgggca agtttttcta
      481 tggtgcactg attattttct ttgccggctt tctggacatg ctggacgggc aggtggcgcg
      541 gcggcagaac cgcgtgacgg cgttcggtgc gttttacgat tcgacgctgg accgctacgc
      601 ggatatggcg ctatacatgg gcctgttggt gtattactcg gtgagcggcc gcacgccgta
      661 tgtcgtgctg gcggcagtgg ctacagcggg ttcggtgatg gtgagctatg cgcgagcgcg
      721 agcagagtcg ctgattccgc tgtgcaaagt gggcttcatg gaacggccgg agcgactggt
      781 gctgttgatc atcggcggcg tgttcaaccg catggcgcca gtgttgtggg taatcgcggt
      841 ggtttctacg ctcacggtga ttcatcgcat cgtgttcacc tggcaagaga cgcgcgcagg
      901 caggacgctc cccggaatca aactcagttt gtaatgtttc gttgcagcgc gtctttgcgg
      961 gcttcagtct acgacttctt gttctgccgg ttgctgttgg gcctccgtta gtcccgccaa
     1021 gaattcatcg atgggctgtt caatcgtttc tttcactcga ttgcgtaatt ccggaacgtc
     1081 ttccttggtg aggccggctg tgtcgatgga cgcgtgaagg aagacgcgga tctcggccgg
     1141 ccagaacatc cagtggccag tacggtgatg tttgaaggat ccgacgatgc tgaccggcac
     1201 aatgggaatt tcaagttgct gggcgacgcg gaacgcaccg tcctgaaatt cggtgacatg
     1261 gccgtcgcgg gtgcgcttcc cttccgggaa aacgatgagg ctggtgccac cgttgatggc
     1321 gtcgcgcgtg aggcgccaca tgcgtttgag atcggagggg cgacgtacga cgggaacagg
     1381 gacgttgccg aaacgcttca tgagccagcc gtaaatgggg atttcaaaat gggattcgag
     1441 ttcccagccg cggacgaact gcggaatcgc gcagtacaaa acgaatggat cgaaaagatt
     1501 gacgtggttg gaaatgaaga aacaggtttt ttgcggatcg aaagcagcat cgcgagcgac
     1561 acgcactctg gcgcccgcaa aaaaagaatg cggcggcaga aggcgcgctg cagccagtcg
     1621 tgtttcttgg gatcgaaaac cagggccagc gcgataagga ttggaacagc gatggcgaaa
     1681 tggagcaggc tgacgaccca gagcagcgcg ctgcgaatga cataccagac acgcctcata
     1741 tgtagacatc cagcgcggca ggaagaatgt cgagcgactt gcacttgagg gtgaggatct
     1801 cgccgtcgat catgacgtcg accggagcgg gaatattgaa ttcgacgtgg cgaacggatt
     1861 tgcgcgaggc gagagggtgt tcgatgtgcg agccgtcata gaggcgcggc agcatgcgga
     1921 gaaggccgag gcggccgata ggaccccagc ggacaaattc gataaagccg tcggaaggat
     1981 cggcttgcgg cgcgatgagc atcgtgccac cagtgtactt gctgttgttg aaggtgagga
     2041 acaggctgcg gcggtcgtcc cagacggttt cgttgtcgca acgcagagtg aaagcgcggc
     2101 gcttgagttg aaccacgcga acgaagacgc cgaggagata accaagatgg ccgagaggtt
     2161 tgaaaaggcg gttggtgatg gcgccgacgt cggcggtgaa gccgaggctg agaatgttga
     2221 aagaatagac ttcgccggtg gcgtgggtga ggcggatgag atcgaccggg cgtttgcgat
     2281 tttcgagcaa ggcctggatg gaggcttcgg agcctgcgcg ggtgaaatcg cggagaaacg
     2341 agttgccggt gccgagcgga agaaaaccga gcgtgatgcg atcgggactg gcgttacgag
     2401 aaaagatgcc gttgaggatt tcatgagcgg tgccatcgcc gcctactgcg agaaagttgc
     2461 ggtagc
//
LOCUS       DASTPM010000737         2749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_949549, whole genome shotgun sequence.
ACCESSION   DASTPM010000737 DASTPM010000000
VERSION     DASTPM010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949549"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VFR42_13030"
     CDS             <1..331
                     /locus_tag="VFR42_13030"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0370133.1"
                     /translation="KLDLGNASCTIGPNGKVKADISAREVVVRGHVDGKVDGTERVQL
                     WNTGRVTGEVRTERLAIEDGAILRGKVEAGKPRGRGTENSTNSAAAKTEKSKSGTADA
                     TSRAAVI"
     gene            336..1094
                     /locus_tag="VFR42_13035"
     CDS             336..1094
                     /locus_tag="VFR42_13035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0370134.1"
                     /translation="MGLLNKLGIGPGGRASRDVSHHSSVGGKAGQHRGGSAPVAAVGP
                     NRSPQADQTNRVSNGLKEFLWNLDGLGRGTLLDLGPAWQTTLNFFIERGFRVTSDDLL
                     HSWKDFLAEEEVRVRNATTSSENIDLTPAGRAARFVQANIQYAPASFDAVLLWDILDY
                     MEPAVAKQTVACLTELLRPGGIVFAMFHSKKPESFQRYRVADSSTLQVIGAAPLVPAQ
                     RVYQNREIQDLFNRFRTVKSFVSRDQLRESLFVK"
     gene            complement(1427..1642)
                     /locus_tag="VFR42_13040"
     CDS             complement(1427..1642)
                     /locus_tag="VFR42_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370135.1"
                     /translation="MKKTLATLLLMGMPAVALAMRPVQEVSQEPATTGSDTARSHHSA
                     KHHKRSKNAHHKSAKRHHTTKQHPQAQ"
     gene            complement(1812..1991)
                     /locus_tag="VFR42_13045"
     CDS             complement(1812..1991)
                     /locus_tag="VFR42_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370136.1"
                     /translation="MAWIVRTLRLPAAESHTNLALVFCVLMMGLMSVAIVWQAQIIAN
                     QRDIIRFLETIKFGG"
     gene            complement(2282..>2749)
                     /locus_tag="VFR42_13050"
     CDS             complement(2282..>2749)
                     /locus_tag="VFR42_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370137.1"
                     /translation="DADIEKFRQEAEHRGLRVQKNSQFWSLSCGANLAKCVRELGALY
                     DRALRGHALRVGLRVLTGDEKQSTRTDGWPAAAFDKTFSLTEHADRREKPEGNVEDQD
                     SLEEAGDSTEIAYGEEAGKSHPAGAPHANRFYLHSPQVWDDVLAAVGTTTLRR"
BASE COUNT          562 a          804 c          815 g          568 t
ORIGIN      
        1 caagctggat ctcggcaacg cctcgtgcac gattggcccc aacggcaaag taaaagccga
       61 catttcggcg cgcgaagtgg tggtacgcgg ccatgtggac ggcaaggtgg atggcactga
      121 acgcgtgcag ctgtggaaca ccggccgagt taccggcgaa gttcgcacgg aacgcctggc
      181 gattgaagac ggtgcaattc tgcgtggcaa agtcgaagcc ggcaaacctc gcggtcgcgg
      241 cacggagaat tcgacaaaca gcgctgctgc gaaaacggag aaatccaaga gcggcactgc
      301 agacgccact tcgcgagcgg cggtaatcta atttcatggg gctgctgaac aaattaggaa
      361 tcggaccggg tgggagagcc agccgggacg tttctcatca ctcgtcggtg ggcggtaaag
      421 ccggccagca tcgcggtggg agcgcgccgg tggcagcggt cgggccgaac cgttcgccgc
      481 aggccgacca gaccaaccgc gtctctaatg gcttgaaaga atttctctgg aacctggacg
      541 gtctcgggcg cggcacgctt ctggatctcg gaccggcctg gcaaaccacg ctgaattttt
      601 tcattgagcg cggattccgc gtcacctcgg acgacctgct gcactcgtgg aaagattttc
      661 tggcggaaga agaagttcgc gtaaggaacg ccacaacgtc gtccgaaaac attgatctga
      721 ccccggctgg ccgtgcggcg cgctttgtcc aggccaacat tcagtacgca ccggcgtcgt
      781 tcgatgccgt cctgctatgg gacattctgg attacatgga gccggccgtc gccaagcaga
      841 ccgtcgcctg tttaacggaa cttctgcggc caggcggaat tgtgttcgcc atgttccaca
      901 gcaaaaagcc ggaaagcttc cagcggtatc gcgtggcgga ttccagcacg ctgcaggtga
      961 tcggcgcggc gccgttggtc ccggcacagc gcgtttacca gaaccgcgaa attcaggacc
     1021 tcttcaatcg cttccgcact gtgaagtcgt ttgtcagccg cgatcagctc cgcgaatcgc
     1081 tcttcgtgaa gtaacgctct ttatccacag cgccgttctt ctctctctga gctcatacat
     1141 ttccgcttgc attacgcgtt gcacgggcgt aaaccgttcc cgtgagctca gcgagccacg
     1201 gaaagagggg catgactcca ccgggcttag agcaaaccac aagctgatca caccgcgcat
     1261 gcggtgcgtg tcgctcgcga atcggcacag caggaacacg cgcaagttcg cagacgaccc
     1321 agcaaaaata gacaaaaagg ggagccgcct ggctcccctt tttgcgtgtt cgttcggaat
     1381 cttctggcga gtggcgtttc gcgcggctta ctcagcttga cgcgccttac tgcgcttggg
     1441 ggtgctgttt cgtggtgtgg tgacgttttg ccgatttgtg atgcgcgttc ttgctgcgct
     1501 tgtggtgctt ggcgctgtga tgcgatcttg cggtgtcgga acccgtggtc gccggttcct
     1561 gcgaaacttc ttgcaccggc cgcatggcca gcgcaaccgc gggcattccc atcagtaaca
     1621 aagttgccag agtctttttc acttgctttt ctcccccaac caaaattccc ctgctggaaa
     1681 aagggtacac caacctagag aaaagtcgag aggccagaaa agggaatgtg ccgcgcaggc
     1741 gagtcagacg aaaaatcagt tcgagcatca tcgttgagct tcggcggggc cagctggaat
     1801 cgcttcgcgg atcagccgcc gaacttgatc gtttccagga accggataat gtcgcgctga
     1861 ttggcaataa tctgtgcctg ccacacgatg gcgacggaca tcaggcccat catcaatacg
     1921 caaaatacca gcgccaggtt ggtgtgcgac tcggctgcgg gaaggcgaag agtgcgaact
     1981 atccaggcca tctgattttc tcctagtacc tatgggccgc gcgtgtagcg tctgggaaca
     2041 gtcagcgcca agtaccaccc atattggagc aatcagtgtt ccaagtgggt ccgcgcggcc
     2101 cgcccgacga acttgccatt tttggccgag aaaattatgg ccgcgcggtg aaactgaatc
     2161 ctaggggcgt accgcgatgc gaagtgtggc cactcggagt gacgtgctgt tctctttttg
     2221 tgaccagccg cggcggcgaa tcttgcagtc gagtcctacg agtaccagcg aaacgcaaac
     2281 gtcagcgccg cagtgtagtc gttcccacgg cggctaaaac gtcgtcccag acttgcggcg
     2341 aatgaagata gaatcggttt gcatgcggcg cgcccgcagg gtgggatttc ccggcctcct
     2401 cgccgtacgc aatttccgtg gagtccccgg cctcctctaa agaatcttga tcctccacgt
     2461 ttccctccgg cttctcgcgg cggtccgcgt gctcggtcag cgagaatgtc ttgtcgaacg
     2521 cagctgctgg ccagccgtcc gttctggtag attgcttttc gtcgccagtc agaacgcgca
     2581 agcccactcg cagcgcatgt ccacgcagag cccggtcgta gagggcgcca agttcgcgaa
     2641 cgcattttgc cagattcgcg ccacacgata gcgaccagaa ttgcgaattc ttttgcactc
     2701 tcaggccgcg gtgctccgct tcttggcgaa acttctcaat gtccgcgtc
//
LOCUS       DASTPM010000738         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_228912, whole genome shotgun sequence.
ACCESSION   DASTPM010000738 DASTPM010000000
VERSION     DASTPM010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228912"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..1454)
                     /locus_tag="VFR42_13055"
     CDS             complement(180..1454)
                     /locus_tag="VFR42_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEU0370138.1"
                     /translation="MSTPVLYEASHDFDIAWVRKQFPSLQLQVNGQPAAFLDGPAGTQ
                     VPRQVMDAVQKYFLEANANTYGGFLTSRRTNEVIAAARAAMADLFHCAPDEVVFGQNM
                     TTITLGLSRAIARELQPGDEILLTTLDHDANFSPWAALAEKGVVIRKVDIRESDCTLD
                     LDDLKSKLNEKTKLVAVGYASNVVGSINPVAEITKAAHAAGALCFIDAVHYAPHGPVD
                     VKALDCDFLVCSPYKFFGPHMGTLYGKKHLLEKFTPYKVRPATDTVPECWETGTQVQE
                     LIAGIHAAVEYIAELGRRCDPSVKARREALLAAYRSTVAYERALVTRLIEGLLAMPGL
                     RFFGITDPQRVEERCSTVSFRLAKGAPTDAATFLGDRGIFTWDGNFYALNLTERLGVE
                     QHGGVLRVGLVHYNTADEVDRLLAALREFASL"
     gene            1650..2303
                     /locus_tag="VFR42_13060"
     CDS             1650..2303
                     /locus_tag="VFR42_13060"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0370139.1"
                     /translation="MPDNAAMSERTRIVREPQRAVYDREVIYKILDQGFVCHVGFTAD
                     GQTYVIPTMYARVGDAIYFHGSAASRMLRGAASAFNVCVTVTLADGLVLARSVFNHSM
                     NYRSVVALGNALIVDEPDEKLEALRAFTEKILPGRWSDARQPNEKELKATSILRLPLD
                     EVSAKVRVGGVEDDAEDYALKVWAGIVPLRLVTDRPVRDERCDAGIRTPEYAERFRK"
BASE COUNT          528 a          566 c          903 g          477 t
ORIGIN      
        1 tgcagagcgt gaaaactgac gaatcgggaa ctccgcttga taagaaaaat gatgagcggg
       61 ccgatccacc atgtcgaaat catcagcgcg tgggccaagc ctgcgtaggc tttttcgtcc
      121 tgagtcagct cggcaggagg aggagttggg atggggttgg gcgggaaggc catggtcatt
      181 tacaaggacg cgaattcgcg gagggcggcg aggaggcgat cgacttcgtc ggcggtattg
      241 taatggacga ggccgacgcg gaggacgccg ccgtgctgtt cgacgccgag gcgttcggtg
      301 agattgaggg cataaaaatt tccgtcccag gtgaagatgc cgcggtcgcc gaggaaggtg
      361 gcggcgtcgg tgggcgcgcc tttggcgagg cgaaacgaaa cggtagagca gcgctcttcg
      421 acgcgttggg gatcggtgat accgaagaag cgcaggccag gcatggcgag cagcccttcg
      481 atgagtcgag tgaccagagc acgttcgtag gcgacggtgg aacggtaagc agcaaggagc
      541 gcttcacgac gggctttgac cgacggatcg caacgccggc cgagttcggc gatgtattcg
      601 acggcggcgt gaattccggc gatgagttcc tgcacctgcg tgccggtttc ccagcattcg
      661 ggcacggtgt ccgtggcggg gcgaactttg tagggagtga atttctccag gagatgcttt
      721 ttcccgtaca gcgtgcccat gtgcgggccg aaaaatttgt aaggcgagca gacgaggaag
      781 tcgcagtcga gggctttgac gtcaacggga ccgtggggcg cgtagtggac ggcgtcaatg
      841 aaacacaggg cgccggcggc gtgggcagct ttggtaattt ccgcgacggg attgatggag
      901 ccgacaacat tggaagcgta gccgacggcg accagttttg ttttttcgtt gagtttactt
      961 ttgaggtcgt cgagatcaag cgtgcaatcg gattcgcgga tgtcaacttt gcggatcacg
     1021 acgccttttt cggcaagtgc ggcccaggga gaaaaattgg cgtcgtgatc gagcgtggtg
     1081 aggagaattt cgtcgccggg ctggagttcg cgggcgatgg cgcgagagag gccgagcgtg
     1141 atggtggtca tattctggcc gaagaccact tcgtctggag cgcaatggaa aaggtcggcc
     1201 atggcggcgc gcgcggcggc aataacttcg ttggtgcggc gactggtcag gaacccgccg
     1261 taggtgtttg cgttggcttc gagaaaatat ttctggacgg catccatgac ctggcgcggg
     1321 acctgtgtgc cggcggggcc atcaaggaag gcagcgggct ggccattaac ctggagttgc
     1381 aacgagggga attgtttgcg aacccaggcg atgtcgaaat cgtgagaagc ttcgtataaa
     1441 acaggtgtgc tcattcgatc ctcgaaagaa tttgagccgt tagtttctca cagacgcgcg
     1501 acatggaaaa cggttaagcg tgatagcatc gtcgagatca gtcagtcatg ccgagcgggc
     1561 gccggctaaa aatcaaacga gcggccatag ctacttgaag ccttcagggg gcgggccggc
     1621 ttcgcgccgc gtttcatcgc gggaaagcaa tgccagacaa tgcagcgatg tccgaacgga
     1681 cgcggattgt gcgcgagccg cagcgcgcgg tctacgaccg cgaagtgatt tacaaaatat
     1741 tggaccaagg attcgtgtgc cacgtgggat tcacggcgga tgggcagacg tatgtcattc
     1801 cgacgatgta cgcgcgggtg ggcgatgcga tttattttca cggatctgcc gcgagccgaa
     1861 tgctgcgcgg cgcggcgtcg gcattcaatg tgtgcgtgac ggtgacgctg gcggacgggt
     1921 tagtgctggc gcgatcggtg ttcaatcatt cgatgaacta ccggtcggtg gtggcgctgg
     1981 ggaatgcgct gattgtggac gagccggacg agaagctgga ggcgctgcgg gcgtttacgg
     2041 aaaagatttt gccgggacga tggagcgatg cgcggcagcc gaacgaaaaa gaattgaagg
     2101 ctacgagcat tttgcgattg ccgctggatg aagtttcggc gaaggtgcga gtcggcgggg
     2161 tggaggatga tgcggaggac tatgcgctga aggtatgggc ggggattgtg ccgctgcggc
     2221 tggtcaccga tcgaccggtg cgtgatgagc ggtgtgatgc ggggattcgg acgccggagt
     2281 atgcggagag attcaggaag tagcagaaag aaaacaaaag aaagacccat tctcacaaaa
     2341 ccgagggtgg gcaccggtag gaaggagccg gccactacag gacggcgcgg aggaagtcgg
     2401 ctaggaggcc gtaggcggtg gcttcgattc cggggttgtg ctcgacgatg gaaaagctga
     2461 agacgtcgag gtcg
//
LOCUS       DASTPM010000739         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1382073, whole genome shotgun sequence.
ACCESSION   DASTPM010000739 DASTPM010000000
VERSION     DASTPM010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382073"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1761)
                     /locus_tag="VFR42_13065"
     CDS             complement(<1..1761)
                     /locus_tag="VFR42_13065"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0370140.1"
                     /translation="MIAIENVSAGPSQYGKSRSGNSPSGNSRRDFLRGVVSAGAFVLA
                     ARYLPAETLSAGAAAGTPDGVADIALKGPLSPSVYLAIDTDGTVYTIAHRSEMGSGSK
                     TALPRIIADELDADWNRVKIVQAVGDEKYGDQDTDGSHSVRGSFDTLRETGAAARLML
                     VHAAAQKWNVPESECSTGLHEVLHKKSGKKAGYGELVTAAAKLEVPKKSDLKLKPRSQ
                     RRYMGKGVSSYDLKDMVRGKATYGIDTRMDGMLYANLAHPPVLGAAVKSLDDKDTLAV
                     PGVKQTATIDPFKPPILFQAHGGVAVLADSTWAAIQGKKKLKIEWTDSEHKSYNSDPY
                     KAELQATCRKPCKVVREIGNVDEAFAKGGKVIEAEYYAPMLAHASMEPPAALASYKDG
                     KVEVWSATQNPQGTREAVASAVGAKKEEVTVNVTLLGGGFGRKSFPDFAIEAAVLSKK
                     TGKPVKVVWSREDDIQFDTFHSVAAMYLKASLGPDGKPTAWLQRSTFPPIASTFDASA
                     LTADAGEIGLGWSDLPFAIPNHRAENGEAKAHVRIGWLRSVSNVFHGYAEHCFADELA
                     HAAGKDPRDYVLALLGPSRII"
     gene            1908..>2497
                     /locus_tag="VFR42_13070"
     CDS             1908..>2497
                     /locus_tag="VFR42_13070"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEU0370141.1"
                     /translation="MWQTTLLVVMVGLLTLLLKENRAQVRYALWLIASLKFLAPYSLL
                     AAAGSYLSWLHPLGAENREISFLIRGPFGAGAVADTNAANGLSFAARLAPVVLLGVWA
                     IGAVAVLFFWWLRWHRTKSILREAAPLTDGRAFDTLRRLEKASGTKRRMALVSGAINL
                     EPGVVGIFRPVLFLPAGVSKELSQEQLESILIHELC"
BASE COUNT          421 a          672 c          846 g          558 t
ORIGIN      
        1 aatgatgcga cttggcccaa gcagggcgag gacgtaatcg cgcggatctt tgcctgcggc
       61 gtgcgcgagc tcgtcggcga agcagtgttc cgcgtagccg tgaaagacgt tcgagacgct
      121 gcgcagccag ccgatgcgga cgtgcgcttt ggcttcgccg ttctccgcgc ggtgattcgg
      181 aatagcgaaa ggcagatcgc tccagccgag gccgatttcg ccggcgtcgg cggtcagcgc
      241 gctggcgtcg aacgtggaag cgatcggcgg aaaggtgctg cgctgcagcc aggcggtcgg
      301 tttgccgtcg ggtccgagtg acgctttcaa atacatcgcg gcgacactgt ggaaggtatc
      361 gaactgaatg tcgtcttcgc ggctccacac tacttttacc ggcttgccgg tttttttgga
      421 gagcaccgcc gcttcgatgg caaaatccgg aaaagacttg cggccaaagc caccgccgag
      481 cagcgtgacg ttgacggtga cctcttcctt tttcgcgccg acagcggagg cgacggcttc
      541 tcgagtgcct tgcggattct gcgtcgcgga ccagacctcg actttgccgt ctttgtagga
      601 agcgagagcg gctggcggct ccatggaagc gtgcgccagc atgggcgcgt agtattcggc
      661 ttcgataact tttccgccct tggcgaacgc ttcgtcgaca ttgccgattt cgcgcactac
      721 tttgcagggc ttccggcagg tggcctgcag ttccgcttta tacggatcgg agttgtacga
      781 cttgtgctcg ctgtcagtcc attcaatctt tagtttcttt tttccctgaa tggcagccca
      841 ggtgctgtcg gcgagaaccg cgacgcctcc gtgcgcctgg aagagtatcg gcggtttgaa
      901 ggggtcgatg gtggcggtct gtttaactcc cggcacggcg agagtgtcct tgtcgtctag
      961 ggatttgacg gcggcgccga gaacgggcgg atgcgcgagg ttggcgtaga gcatgccgtc
     1021 catgcgggta tcaattccgt aggtggcctt gccgcgcacc atatctttca agtcgtaact
     1081 ggaaacgcct ttgcccatgt agcgccgctg gctgcgcggc ttgagcttga ggtcgctctt
     1141 tttaggaact tcgagtttcg ccgcggcagt gaccagttcg ccgtagccgg ctttcttgcc
     1201 ggattttttg tgcagcactt cgtggaggcc ggtggagcat tccgattcgg gaacgttcca
     1261 cttctgtgcg gcggcgtgca ccagcatcaa gcgagccgcg gcgccggttt cgcggagcgt
     1321 gtcgaacgaa ccgcgcacgg agtgagaacc gtcggtgtcc tgatcgccat atttttcgtc
     1381 gccaacggct tgaacgattt ttacgcggtt ccaatcggca tcaagctcat cggcgatgat
     1441 gcgtgggaga gcggtcttgc tgccgctgcc catttcggag cgatgcgcga tggtgtagac
     1501 agtgccgtca gtgtcgatgg cgaggtagac gctgggcgac agcggacctt tgagagcgat
     1561 gtcggcaacg ccgtcgggag tgccggccgc cgctccggcg gagagagttt cggctggaag
     1621 gtagcgagcg gcgaggacga aggcgcccgc gctcaccacg ccgcgcagaa agtcgcgccg
     1681 agaattaccg gacggagagt tcccggaccg ggatttgcca tattgagatg gacctgcgga
     1741 cacgttttcg atggcgatca cgaaaccctc cggcttttcg gtgattttca gagcttcatg
     1801 gtaacgctat ttcggatttc tgcaaattgg cgggcgaccg gcggaatgca cgcccgggcg
     1861 accggcgaca ggcgacaggt ggtctctgga ggttagcagc ggagcacttg tggcaaacga
     1921 cgctgttagt ggtgatggtc ggattgctga cgttgctgct gaaggagaac cgcgcgcaag
     1981 tacgttatgc gttgtggctg atcgcctcgc tcaaatttct ggccccttac tcgttactgg
     2041 cggcggcggg gagttacctt tcgtggttgc atccattggg agcggagaat cgggaaatct
     2101 cttttctgat tcgcgggccg tttggcgcgg gcgcggtggc ggataccaat gccgcgaacg
     2161 gactgagctt tgcggcgcgc ctggcgccag tggttttgct gggcgtgtgg gcgatcggag
     2221 ctgtggcggt cttgtttttc tggtggctgc ggtggcaccg gacaaagtcg attctgcggg
     2281 aggcggcgcc gcttaccgat ggtcgcgcat tcgacacgct gcgcaggttg gagaaagcca
     2341 gcgggacgaa gcgtcgcatg gcgctggtga gcggagcgat caatctcgaa cccggggtgg
     2401 tcggaatttt tcgcccggta ctgtttcttc ctgcgggagt ttcgaaagag ctatcgcaag
     2461 agcagctgga gtcgatcctg atccatgaac tgtgcca
//
LOCUS       DASTPM010000740         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_314928, whole genome shotgun sequence.
ACCESSION   DASTPM010000740 DASTPM010000000
VERSION     DASTPM010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314928"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VFR42_13075"
     CDS             complement(<1..456)
                     /locus_tag="VFR42_13075"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU0370142.1"
                     /translation="MAEEKLPTRSQDFSEWYNQLVLKAQLADYAPVRGCMIVRPYGWA
                     LWENVQQALDRRFKATGHQNVAFPLLIPRSFIDKEKHHVEGFSPELAVVTVGGGEELA
                     EPLVIRPTSETIIGHMWSKWILSYRDVPVLMNQWNSVVRWELRTKLFLRT"
     gene            complement(803..2038)
                     /locus_tag="VFR42_13080"
     CDS             complement(803..2038)
                     /locus_tag="VFR42_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEU0370143.1"
                     /translation="MGHPLPGRPRIAERILAWLESREDEMASLLAELVRVPTENPPGN
                     HYRECADLLEKKLNEAGLACERHLFAGKAGAPDAAPSLIATYGSGPRTLFFHGHYDVV
                     PAQSPDQFNPLRKEHFLFGRGSCDMKGGIVAMLYAIRALQECKIQLAGKIALTLVPDE
                     ETGGARGSEWLAREGKLGREGIGMLLAEPTSGVVWDANRGAISLRVTVHGKSAHVGLQ
                     HRGENAFEQMVRVVERLQELKREVEQRKTTSTVGADQLPNSILLLGGESGGGTNFNVV
                     PEQYWFTLDRRINPEENFDAEKYRILHLLEDCKRDGIRLQWEILQAGQASACPEKEKL
                     GQALAQSVKEISGQAPRFEMCPGLLELRFYAALGIPAYAYGPGLLSVAHGPSEYVDLR
                     KVRECAAIYALTAMEMLTK"
     gene            complement(2153..>2416)
                     /locus_tag="VFR42_13085"
     CDS             complement(2153..>2416)
                     /locus_tag="VFR42_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370144.1"
                     /translation="AATMIAAPSYPHYTAAHFPALAVSQLFSVSLCLCGNPAFLQRPG
                     SSCGEIKTVPPPRVFLCKSVDLLENKGVAIFDKHKERKEFVIE"
BASE COUNT          495 a          740 c          698 g          483 t
ORIGIN      
        1 cgtgcgcaga aaaagtttgg tgcgcagttc ccagcgaacc acgctgttcc attgattcat
       61 cagcaccggc acgtcgcggt aagacagaat ccatttcgac cacatgtgcc cgatgatggt
      121 ttccgaagtc ggccggatca ccagcggctc ggccagctct tccccgccgc cgacagtgac
      181 caccgccagc tccggcgaaa atccctcgac gtgatgcttc tctttgtcga tgaagctgcg
      241 cggaatcagc agcggaaacg ccacgttctg gtgcccggtg gccttaaatc gccggtccag
      301 cgcctgctgc acgttctccc acagcgccca cccgtaaggc cgcacaatca tgcacccgcg
      361 caccggcgca tagtcggcca gctgcgcctt caacaccagc tggttgtacc actccgaaaa
      421 atcctgcgag cgggtaggaa gcttctcttc agccattcaa caatttgtcc aatgtggccc
      481 aggtcaccga cccaagccgg tctagttgca cgcagcaaaa tcgcgccatc cgcgaaccca
      541 aaagcctaac gaacactccc aaccctgtca aaccaagcgc ccgaaagcaa cgcgaagcaa
      601 cccacgtcgc cgttcgtgga gtgcggcggt cttgccgccg ctttcgccat gaacagtgct
      661 aagggcgacg ccaacattgt ggctaccaat gcagacgtaa gtagctccag caccggccag
      721 agtcttcgtg gccctgcgac caccgggcgc aggaaaaagc cgggtcgctg cgcggtcggg
      781 atgacaggat caagaatcga tttcacttgg tgagcatctc catcgcggtc agggcgtaga
      841 ttgcggcgca ttcgcggact ttgcgcaggt ccacgtattc gcttggtccg tgagcaaccg
      901 aaagcaggcc tgggccgtag gcataggcgg gaattccgag cgcggcgtaa aaacgaagtt
      961 ccagcaggcc cgggcacatt tcaaaacgcg gcgcctggcc actgatctct ttcacacttt
     1021 gcgcgagcgc ctggccgagt ttttctttct ccgggcacgc cgatgcctgg ccggcctgga
     1081 gaatttccca ctgcaagcga attccgtcgc gcttgcaatc ctccagcaga tggaggatgc
     1141 ggtatttttc cgcgtcgaaa ttttcttcgg gattgatgcg gcggtcgagc gtaaaccagt
     1201 attgctccgg gactacgttg aagttggttc ctccgccgct ttcgccgccc agcagcaaaa
     1261 tggaattggg caactggtct gcgccgacgg tcgaagtggt tttgcgctgc tcgacttcgc
     1321 gtttcaattc ctgcaaacgt tccaccacac gcaccatctg ctcgaaggcg ttttcgccgc
     1381 gatgctgcaa gccgacgtgc gcggatttgc cgtgcacagt aacgcgcagc gaaatcgcgc
     1441 cgcgattggc gtcccacacc acgccgctgg tgggctcggc cagcagcatg ccgattccct
     1501 cgcggcccag ctttccttca cgcgccaacc actccgagcc gcgggcccct ccggtttctt
     1561 catcggggac gagggtcaga gcaatcttgc cggccagctg aattttgcat tcctgcagcg
     1621 cgcgaatggc gtacagcatg gcgacgatgc cgcccttcat gtcgcaggaa ccgcggccaa
     1681 ataaaaagtg ttccttgcgc agcggattga attgatccgg agattgcgca gggactacgt
     1741 cgtagtgccc gtgaaagaaa agtgtgcgcg ggccgctgcc ataggtggcg atcaggctgg
     1801 gcgcagcgtc aggcgctccg gctttgccgg caaacagatg gcgttcgcaa gcgaggccag
     1861 cctcgttcag tttcttctcc aggaggtcgg cgcattcgcg atagtgattg cccggcgggt
     1921 tctccgtggg gacgcgcacc aattcggcga gcaggctggc catctcgtct tcgcgagatt
     1981 caagccaggc aaggattcgt tccgcgatgc gcggacggcc tgggagaggg tgacccacgc
     2041 gtttcatcct aggaatacgc cgtagtgtcc gccaaggtcc aaaatggttc gacgaggaag
     2101 aagctggtcg tcggctatcc tgcgtattca tcccacctca actcttttat tttcattcga
     2161 ttacgaactc tttgcgctct ttgtgcttat cgaaaatcgc aaccccttta ttttcaagca
     2221 aatccacact cttgcacaaa aacacccggg gtgggggtac cgtctttatc tcgccgcacg
     2281 agcttcctgg acgctgaaga aaagcggggt tgccacagag acacagagac acagagaaaa
     2341 gctgggaaac ggcaagggcc gggaaatggg cggcggtgta gtgtgggtat gacggggcgg
     2401 cgatcatggt tgcggc
//
LOCUS       DASTPM010000741         7875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_516993, whole genome shotgun sequence.
ACCESSION   DASTPM010000741 DASTPM010000000
VERSION     DASTPM010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516993"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1026)
                     /locus_tag="VFR42_13090"
     CDS             complement(133..1026)
                     /locus_tag="VFR42_13090"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU0370145.1"
                     /translation="MSRRIVGFLAIFLAVACAAVALVWLHAGGQNKTTVNFTPDPPAI
                     TNSNAPTAAPQPAAATTPPATKQPAAAPAEPAGFAPPIGEALEFSASVAKVNNVATLR
                     LSVNGRKEIAGKDAWHLQAFAHTQNPLRLMFELDDQFDSYSAPGDFTSMQYEMRLSER
                     GTKVNSIQRMTATGREPAGADATQARVLPGTRDPLGMMQYLRGVDWAKTPEVHGPVYD
                     GRKIYTVKAEKIGSAEVQVPAGKFSTTTVEIKVFDNGAEMKDAHFTLYLAKDAARTPV
                     LLEAVLPFAEARVQLMKKNGQ"
     gene            1237..2202
                     /locus_tag="VFR42_13095"
     CDS             1237..2202
                     /locus_tag="VFR42_13095"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0370146.1"
                     /translation="MRYVVTGGAGFIGSNIVDELVRRGESVVVLDDLSAGKEDNLAEA
                     RNKISFIKGSINDIEAVRRSMQEADYVIHLAARTSVPRSVKDPVETNRVNIDGTLNVL
                     MAARDAKVKRLVIAASSSAYGETPTLPKAETMQPEPISPYGVTKYVCELYAQVFGRVY
                     GLQSVSLRYFNVFGPRQDPSSPYSGVLSKFCTAFLEGSEPVVFGDGHQTRDFTYIENV
                     LQATLLACEAPAASGKVINVGTGERMSLNQTLELLREISGNALEAKYEPTRDGDIRDS
                     QADITLGRELLGYEPTVNFADGLRRTFAWYQESQGKALDKAKTEN"
     gene            complement(2324..5842)
                     /locus_tag="VFR42_13100"
     CDS             complement(2324..5842)
                     /locus_tag="VFR42_13100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0370147.1"
                     /translation="MNGKQFALRSILICSIMVALNLAGSAKITRAQSPDIPQDPPQAL
                     SADSDQEKDKDKKDEKKDEKKDEKKGLPLKSDRKVTFTTDEGTWLSLDVSPDGKTIAF
                     ELLGDIYTLPVEGGKAKLIDGGMSFDSQPRFSPDGQWIAFLSDREGSENVWIMKTDGS
                     GVKQVSKDPNNEFASPSWDPSGKYIYVSKTKFGIGPREIWMYHVDGGSGIQITKSRPS
                     PATPRDQRTSDMGVVASPDGRYLYFSQRRRDFSYNSMFPVWSIKRKDRKTGDEDTIIE
                     QPRSAMRPGISPDGKSLLYVTRHETESGLRLRNLETGEDHWVRYPVTRDDQEAIFSRD
                     LFPGYAFLPGGKEIIYNQDGKIRRLNLDTAAESVIPFSAEVSQDLGPKLDFPQKVDQG
                     PVKVRLIHDPAESLDGKKLAFSAMTHLYTLDLPSGKAQQLDTGKSTAFQPAFSPDGKW
                     IAYVSWSSEGGQLWKIPASGGTPVQLSKSLAVYSNPIFSPDGSRIVLLRGNAYDRENS
                     GFDGGQTQNDDLVWISADGGEPNLILPARGAGGPHFTHEKDRVYVYTPGGLISLRYDG
                     TDRRTHLIVKGSGIYFADEPVPADDLVPSPDGQWVLAHVMNQLYIIAMPPVGGEPPTV
                     SVGGPAVPLKKITDIGADYYGWADDGKTVTWAVGASYFRQPLSSISFEAPKDEKKEGD
                     KKDGDKKEEAKDADKDKDKKETADAKQTDKKDEKKDEKKEEKKFKEREKDVQEVKVDL
                     EMPRKTPKGTIVLRGATVVTMKGDEVLKDADIVVENNRIKSVGARGPAPSGAKVFDVK
                     GKTITPGFIDTHAHWFEIKRGILDTQNWAFLANLAYGVTAGLDVQTGTNDMFAYQDLV
                     DTGGILGQRAFSTGPGVFSDNNFQSMEEVKGVLTKYRDYYGTHNIKSYLVGTRKQRQY
                     MVQASKELEMMPTTEGALDLKLDLTHVIDGFHGNEHTLPVTPLYNDVIQMFAKSGIAE
                     TPTLIVNYGGPFGEDYWYEFSEVHDNAKLNHFTPHRIIDQKTKRRPAWFRKDEYEFPK
                     LAAQVAKLERAGGLVGVGSHGQLQGLGYHWEVWMLASGGMTPLEALKCATVNGSKIVG
                     RPDEIGTLEPGKLADLVIFDKNPLDDIHNTNTIHWVMKNGELFEGDTLNQVWPEQKKL
                     EPLWFWNKYDEPKAGDSLGYGKTTTP"
     gene            complement(6172..>7875)
                     /locus_tag="VFR42_13105"
     CDS             complement(6172..>7875)
                     /locus_tag="VFR42_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370148.1"
                     /translation="ESPLTPNVLWAGTDDGNVQLTRDGGKTWTNVAGKVPGVPKGTYV
                     SRVVASRSAEGAAFITFDGHRSDDYSIYIFATADYGETWKPIRAGIPDSAGSVHVVRE
                     HPRNSNLLFAGTEFGLWISWDRGANWTAFKNNFPTVPVDDIELQATYNDLVLATHGRS
                     IWIFDDLTPIEKMDSSVASGDFTFFPPRSTNVWFITNRRWSAGQKMFVAKNPLPGAIL
                     NYYLKSALPPEEPKKESDQEKEAEKSEKAAATQAAQVAPKKDEPVPQQKETGAAEKAK
                     QPIQAADKEEKPEAEKKEGQVKITVTDKDGKVIRELNGPAAAGVNRASWDLRYNSPAE
                     PSPEQLEAMAAGYDFGPRGPFVEPGEYTIKIKAGDKEATQKVTVEADPRLPLNAEDRV
                     ARRAAIDQLYAMAKSTDKDRKTILGIQSALTSARESWKKEADKKDGLKIPAEIVKSAD
                     DLQKKVNDVALKYRREQQGLGNAGPPFEWRPDPLPSQVQDLMQDLDEFAAAPGGQQKE
                     KLAELTPLVADASAQVKKISDEDLPALNKRMNEAGIPHIVPTPPSSSGRRGGSDEDDD
                     L"
BASE COUNT         1481 a         2153 c         2395 g         1846 t
ORIGIN      
        1 cgccgcgcat taccctttgg ttgccggact tatacgcgcg cgtcctttgc ttgccataca
       61 tgcgaaaaat accgggggcc cagtttgtcg cagcgggaga gcgttccatt tcagaacgca
      121 ggacgatcta cgtcactggc cgtttttctt catcaactgg acccgcgctt ccgcgaacgg
      181 cagcaccgcc tccagcagca ccggcgtcct cgctgcatcc ttggccagat agagcgtaaa
      241 atgcgcgtct ttcatttccg cgccattgtc gaaaactttg atttcaaccg tcgtggtcga
      301 aaactttccc gccggcactt gcacttccgc cgacccgatc ttctccgcct tcaccgtgta
      361 gatcttccgc ccgtcataca ccggaccgtg cacttccggc gttttcgccc agtccactcc
      421 gcgcagatac tgcatcatcc ccagcgggtc gcgcgtcccc ggcaatacgc gcgcctgcgt
      481 cgcgtccgcg ccggctggct cgcgccccgt ggccgtcatt ctctgaatgg aatttacttt
      541 tgtgccgcgc tcgctcaacc gcatttcata ttgcatgctg gtgaaatcgc ccggcgcgct
      601 gtaggaatca aactggtcat ccagttcaaa catcaggcgc agcggattct gggtgtgcgc
      661 aaatgcctgc agatgccagg cgtcttttcc cgcgatttct ttgcggccgt tcaccgacaa
      721 ccgcagagta gccacgttat tgactttggc cacgctggcc gaaaactcca gagcctcgcc
      781 aatcggggga gcaaatcctg ccggttcggc gggcgctgcg gctggctgct ttgtcgctgg
      841 cggcgtcgtg gcagcggcgg gctgcggcgc tgctgttggc gcgttggaat ttgtgatggc
      901 cggcgggtcc ggcgtgaagt tcactgtcgt cttgttttgt ccgccggcgt gcagccacac
      961 caacgccaca gcagcgcagg ctacggccag aaaaattgcc agaaatccca ctatcctgcg
     1021 gctcatgtct ctctggtacc ctcgaacttc cgtgaaatct tgtccgcgcg gcttccggcg
     1081 tacaataaac cgtcgcgcgc aggagcgtca gagaacgcgc gggctatggc gccgtgaggc
     1141 gcgagaactc aggttaccag aagcaatttc gtgcgtctcc cgaaagctct ctgggcttgg
     1201 ccggctcggc gcaaaacggt tgggaggacg gctcgaatgc gatacgtagt caccggtggc
     1261 gccggcttca tcggctccaa cattgtggat gaactggtgc gccgcggcga aagcgtggtg
     1321 gtgctcgacg atctttccgc aggcaaggaa gacaatctag ccgaagcccg caataaaatt
     1381 tccttcatca aaggttccat caacgacatc gaagcggttc gccgctcgat gcaagaagcc
     1441 gattatgtga ttcatctggc cgcgcgcact tctgttccgc gctccgtgaa ggacccggtg
     1501 gaaaccaatc gtgtcaatat tgacggcact ctgaatgtgc tgatggctgc gcgcgacgcc
     1561 aaagtcaaac gcctcgttat cgccgcttct tcctccgctt acggcgaaac gccaacgctc
     1621 cccaaagcgg aaaccatgca gccggagccg atctctccat acggtgtcac gaaatacgtt
     1681 tgcgagctct acgcgcaggt gtttggccgc gtctacggcc tgcaaagtgt cagcttgcgc
     1741 tacttcaacg tgttcggacc gcgacaagat ccgagttcgc cttactccgg agtgctctcc
     1801 aaattctgca cggcttttct agaaggtagt gaaccggtag tgttcggcga cggccatcag
     1861 actcgcgact tcacgtacat tgaaaatgta ctgcaggcca cgctgctagc gtgcgaagcg
     1921 ccggccgcga gcggaaaagt gatcaacgtg ggcactggcg aacgcatgtc gctgaatcaa
     1981 acgctggaat tgttgcggga aatcagcggc aacgcgctag aagcaaaata cgagcccacg
     2041 cgcgacggcg acatccgcga ttcgcaagcc gatatcaccc tgggccgcga actgctcggc
     2101 tacgaaccca ccgtaaattt tgccgacggc ctccgccgca ccttcgcctg gtatcaggaa
     2161 tcgcaaggca aggctctaga caaagcaaaa acggaaaatt gaagttggaa aatgggcgga
     2221 tctgagcgcg ccaactcgat tcaaaacaac tcggtcgagc acctgggcgc ttgaccgagt
     2281 ttcgtttttc gagtttcgtt tttctatttt ctactttctg atttcacggg gttgtggttt
     2341 tgccgtaacc gagcgagtcg ccagctttcg gctcgtcgta tttgttccag aaccacagcg
     2401 gctcgagttt cttctgttcc ggccaaactt gattgagcgt atcgccttca aacagttcgc
     2461 cgttcttcat cacccagtga atggtgttgg tgttgtgaat gtcgtcgagc gggttcttgt
     2521 cgaagatcac caggtcggcg agcttgcccg gttcgagagt gccgatttcg tcaggccggc
     2581 cgacgatttt tgatccgttg accgttgcac acttcagcgc ttccagagga gtcatgccgc
     2641 cgctggccag catccacact tcccagtggt agccgagtcc ctgcaattgg ccgtgactgc
     2701 cgacgccgac caatccgcca gcgcgttcca gtttggcgac ttgagccgcc agtttcggga
     2761 attcgtactc gtctttgcgg aaccaggcgg gacgccgctt ggtcttttgg tcgatgatgc
     2821 gatgcggtgt gaagtggttg agcttggcgt tgtcgtgcac ttcgctgaac tcataccaat
     2881 aatcttcgcc gaatggcccg ccgtagttca cgatgagcgt cggagtttcc gcgataccgg
     2941 acttggcgaa catctggatg acgtcgttgt agagcggcgt gaccggcaac gtgtgttcgt
     3001 tgccgtggaa gccgtcaatc acgtgagtaa gatccagctt caggtcgagc gcgccttcgg
     3061 tggttggcat catctcgagt tccttggaag cctggaccat gtactggcgc tgcttgcgcg
     3121 taccgaccag gtagctcttg atgttgtgcg tgccgtaata atcgcgatac ttggtgagca
     3181 cacctttcac ttcttccatg gactggaagt tgttgtcgga gaaaacgccg ggcccggtgg
     3241 agaaggcacg ctggccaagg atgccgccgg tgtccaccag atcctgatag gcgaacatgt
     3301 cgttggttcc ggtctgcaca tcgaggccgg cagtgacgcc gtaggcgaga ttcgccagga
     3361 aggcccagtt ctgcgtatcc aaaatgccgc gcttgatttc aaaccagtgc gcatgggtgt
     3421 caatgaatcc gggcgtgatg gtcttacctt tcacatcaaa aactttcgcg ccagagggag
     3481 cagggccgcg ggcgcccaca ctcttgatgc gattgttttc gacgacgatg tcggcgtcct
     3541 tgagaacttc atcgcccttc atggtgacaa cggtggcccc gcgaaggacg atagtaccct
     3601 tgggagtctt gcgcggcatc tcgagatcca ctttcacttc ctggacgtct ttttcgcgtt
     3661 ccttgaattt cttttcttcc ttcttttcgt ctttcttctc gtcctttttg tcggtttgtt
     3721 tggcgtccgc agtctccttc ttgtctttat ccttgtcggc gtctttggct tcctcttttt
     3781 tgtcgccgtc cttcttatcg ccttctttct tttcgtcctt gggggcttcg aaggaaatgg
     3841 aactgagcgg ctgccggaaa tagctggcgc ctacggccca ggtgacggtt ttgccgtcat
     3901 cggcccagcc gtagtagtcg gcgccgatgt cggtgatttt cttcaacgga acagccgggc
     3961 cgccgacgct caccgtcggc ggttctccac cgactggcgg catggcgatg atgtaaagct
     4021 ggttcatgac atgggcaagt acccactggc catcggggct gggaaccaga tcgtcagcgg
     4081 gaaccggttc atcggcaaag tagatgccgg aacccttgac gattaaatga gtgcggcgat
     4141 cggtcccgtc ataacgaagg gatatcaatc cgccgggcgt gtacacataa acgcgatctt
     4201 tttcatgagt gaaatgaggg ccgccggcgc cgcgagccgg cagaatcaga ttgggttcgc
     4261 cgccatcggc ggaaatccag acgagatcgt cgttctgcgt ctggccgcca tcgaagccgc
     4321 tgttttcgcg gtcgtaggca ttgccgcgga gcagcacgat gcgcgagcca tccggagaaa
     4381 agatgggatt gctgtagacg gcgagcgatt tcgagagttg tacgggagtg ccgcccgacg
     4441 cgggaatctt ccaaagctgc ccgccttcgc tggaccagga gacataggcg atccacttgc
     4501 cgtcaggaga aaacgccggc tggaacgcag tggattttcc ggtgtcgagc tgctgcgctt
     4561 ttccggaagg gagatcgagc gtgtagagat gcgtcatcgc agagaaggca agctttttgc
     4621 cgtcaagcga ttcggcggga tcgtgaatga gacggacctt gaccggacct tggtctacct
     4681 tttgcgggaa atcgagtttg gggccgaggt cctgggagac ttcggcggaa aaaggaatga
     4741 cggattcggc ggcggtgtcg aggttcaagc ggcggatttt accgtcctgg ttgtagatga
     4801 tttccttgcc gcccggcagg aaggcgtaac cggggaaaag gtcgcgggaa aagatggctt
     4861 cctgatcgtc gcgggtgacc ggatagcgga cccagtggtc ttcgccggtt tcgagattgc
     4921 ggaggcgcaa gccgctctcc gtttcgtggc gcgtgacgta caagagagat ttaccgtcgg
     4981 gggaaattcc ggggcgcatg gcgctgcgag gctgttcgat gatggtgtct tcatcgccgg
     5041 tcttgcggtc tttgcgcttg atggaccaga cgggaaacat cgagttgtag gagaaatcgc
     5101 gcctgcgctg ggagaaatag agatagcggc cgtccggcga ggcgacgaca cccatatcgc
     5161 tggtgcgctg gtcacgcggc gtggctggag aaggccggga tttggtgatt tgaattccgc
     5221 tgccgccgtc cacgtggtac atccagattt cgcgcgggcc gatgccgaat ttggttttgg
     5281 aaacgtagat atatttgccg gacgggtccc aggaaggaga cgcgaattcg ttgttggggt
     5341 ctttggagac ttgcttcacg ccgctgccgt cggtcttcat gatccacacg ttttcgctgc
     5401 cttcgcgatc ggaaaggaag gcgatccact ggccgtcggg agagaagcgt ggctggctgt
     5461 caaacgacat gccaccgtcg atgagcttgg ctttgccgcc ttcgacgggg agagtgtaga
     5521 tgtcgccgag gagttcgaaa gcgatggttt ttccatcggg agagacgtcg agcgaaagcc
     5581 aggtgccctc atcggtggtg aaggtaactt tgcggtcgga tttgagaggc agacccttct
     5641 tctcgtcctt tttctcgtct tttttttcgt ccttcttgtc tttgtctttt tcctgatcgg
     5701 aatcggcgga gagagcctgc ggaggatcct gcgggatatc gggagattgc gcccgcgtga
     5761 ttttggcgga gccagcgagg tttaaggcca ccataatcga gcagattaga atcgaccgca
     5821 gagcgaattg cttaccgttc atgcttctcc ctccttcaaa gacaagtacg atcgctgcaa
     5881 ctggaactcg gacataacct caaggcaagg cagcgaccct ttcgtgcctc agggcaagcg
     5941 aaagccggcc acgacaacgc gtcagagtcg cgaacgaaag gcgtataaga atgcctttcg
     6001 cgagctattg acgcaagcgg ctagcgccgc gcaaagaatc tgggacgaga ttgatcgaac
     6061 cgcacccgca tttctcgact tagacgcgct aagagggcaa tggtttcgca gtcgccaaga
     6121 accttttcag ccgcgaagag gttcgttggt ctgcaagtat gtccttcacc gctagaggtc
     6181 atcatcctcg tcggagccac cacggcgacc ggacgacgaa ggaggcgtcg ggacgatgtg
     6241 gggaatgccg gcctcattca ttctcttgtt gagagccggc aaatcctcgt ctgagatttt
     6301 ctttacttga gcggaagcgt cggcaaccag aggtgtcagc tcggcgagtt tttctttctg
     6361 ctggccacca ggagcggctg cgaattcatc caggtcctgc atcaggtcct gcacttgcga
     6421 tgggagcgga tcggggcgcc actcgaaggg agggccggca ttgccgaggc cttgctgttc
     6481 ccgccgatat ttgagagcga cgtcgttgac ttttttctgc aggtcgtcgg cggacttgac
     6541 gatttcggcg ggaattttaa ggccgtcttt cttatcggct tcttttttcc aggattcgcg
     6601 agcggaggtc agcgcggact ggatgccgag aatggttttg cggtccttgt ccgtggactt
     6661 agccatagcg tagagctgat cgattgcagc gcgccgagcg acgcgatctt ctgcgttgag
     6721 cgggaggcgc ggatcggctt ccacggtgac tttctgcgtg gcttccttgt cgcctgcttt
     6781 gattttgatg gtgtattcgc cgggttcaac gaaaggaccg cgcgggccaa aatcgtaacc
     6841 ggcagccatg gcttcgagct gttccggaga tggctccgca ggcgagttgt agcggagatc
     6901 ccagctggca cgattcacgc cggccgcggc ggggccattg agttcgcgaa tgactttgcc
     6961 gtccttgtcg gtaacggtga ttttaacttg gccttctttt ttctcggctt cgggtttttc
     7021 ttccttgtcg gcagcctgaa ttggctgttt tgctttttca gcggcgccgg tttctttctg
     7081 ctgcggaaca ggctcatctt tcttcggcgc gacttgagcc gcttgggttg cagcggcctt
     7141 ttctgatttc tcggcttctt tttcctggtc ggattccttt ttcggctctt cgggcggcag
     7201 cgcagatttc aggtaatagt taaggatggc gcccggcaga ggatttttag cgacaaacat
     7261 tttttgtccg gcgctccagc ggcggttggt gatgaaccaa acgttggtgc tgcgcggggg
     7321 aaagaaagtg aaatcgccgg aggcgaccga ggagtccatt ttttcgattg gagtgaggtc
     7381 gtcgaagatc cagatggagc ggccgtgcgt ggcgagaacg agatcgttgt aggtagcttg
     7441 aagctcgatg tcgtccacgg gaacggtggg gaaattattt ttgaaggcgg tccagttagc
     7501 gccgcggtcc caagaaatcc agaggccgaa ttcggtcccg gcgaaaagaa gattgctgtt
     7561 gcgcggatgt tcgcgaacga cgtggacgga gccggcggaa tcgggaatgc cggcgcggat
     7621 gggcttccaa gtttcgccgt agtcggcggt agcgaagata tagatgctgt agtcgtcgct
     7681 gcgatggccg tcgaaagtga tgaaagcagc gccttccgca gacctggagg ccacaacgcg
     7741 ggagacgtag gtgcctttgg gaacgccggg aactttgccg gcgacgttgg tccaggtttt
     7801 gccgccatcg cgcgtgagct ggacgttgcc gtcgtcggtg cccgcccaga gaacgttggg
     7861 agtgagcggc gactc
//
LOCUS       DASTPM010000742         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_863085, whole genome shotgun sequence.
ACCESSION   DASTPM010000742 DASTPM010000000
VERSION     DASTPM010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863085"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /locus_tag="VFR42_13110"
     CDS             complement(<1..1735)
                     /locus_tag="VFR42_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU0370149.1"
                     /translation="MERDPVCGMTVDPAKAAAQVEHGGKAYYFCAPGCAKEFSANPET
                     YLLRIVTPQAPPSPFPIHHAAAPAHAQAQNKIRYTCPMHPEVIQFAAGTCPECGMALE
                     PMDFLPNDSSEAADPEYLSMRKRFWVSAALSLPVVLLAMFGEHIALPLSGSARHGIQF
                     ALASPVVMWGGWPFFERFWASLVNRSPNMFTLIGLGTGAAFLDSAVATVFPRIFPASF
                     REMNGSVPVYFEAAAVITTLVLLGQVLELRARAKTSSAIRALLDLAPQVAHRLDKNSA
                     ESDVLLDQVQTGDRLRVRPGERVPVDGVVLDGASAVDESMLTGEPMPVEKSANDKVTG
                     GTLNTSGSFVMRAERVGSESTLAQIVKLVSEAQRSRAPMQRLADQVAAIFVPAVIVAA
                     ILTFIVWSLAGPPPRFAHALVNAVAVLIIACPCALGLATPMSIMVAVGKGAHAGVLVR
                     NAEALEKLEAVDTLVVDKTGTLTEGKPIVRTVMALEGRGLSEDSLLGLAASVEQLSEH
                     PLARAIVRAAEEKNLQIVGVKDFRATAGGGVQADVEGAHVAVGTLTFLEETGLQETSL
                     AHPQHEEAGYST"
     gene            complement(1768..2133)
                     /locus_tag="VFR42_13115"
     CDS             complement(1768..2133)
                     /locus_tag="VFR42_13115"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU0370150.1"
                     /translation="MAHTSNSKSETTAHGADCACRIGPGHKALAVDPALKESNRKRLR
                     RIEGQIRGLQKMVEDDRYCADIITQIAAAQEALRGVARNLMKNHLHHCAAKALRSAKK
                     EQADAMYDELLGLIYSHLR"
     gene            2242..2463
                     /locus_tag="VFR42_13120"
     CDS             2242..2463
                     /locus_tag="VFR42_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370151.1"
                     /translation="MTLLEIRYALQSPLNAEQLRNLSEFANTYGLRKFKLNEAKTELS
                     FEYDASRLRETQVAHALGLAKIAVVKKLN"
BASE COUNT          554 a          843 c          775 g          568 t
ORIGIN      
        1 ccgtagagta gcctgcttcc tcatgctgag gatgtgccaa tgatgtttct tgcaggccag
       61 tttcttccaa aaacgtcagc gttccgacgg cgacgtgggc gccctccaca tcagcctgaa
      121 caccgccgcc agccgtggcg cgaaagtcct tgactccgac gatctgtagg ttcttttctt
      181 ccgcagcacg gacgatcgcg cgcgccagcg gatgctcgct aagctgctca acgctggccg
      241 cgaggccaag taggctgtct tccgacagcc cgcggccttc taaagccatg acggtccgca
      301 cgatgggctt gccttcggtg agcgtccccg ttttgtctac aaccagagtg tctacggcct
      361 caagtttttc gagggcttcg gcgttacgta ccagaacgcc ggcgtgcgcg cctttcccta
      421 ccgcaaccat gattgacatt ggcgtggcca gccccaaggc gcaggggcat gcaatgatca
      481 gaaccgctac cgcgttgaca agcgcgtgtg caaagcgggg cggcggcccg gcgagtgacc
      541 acacgatgaa ggtaagaatg gccgccacaa tcaccgctgg cacaaaaatc gcggcgacct
      601 gatcggccaa ccgttgcatg ggcgcgcggc tgcgttgggc ttcactgacg agtttcacga
      661 tttgcgcgag tgtgctttcg ctgccgactc gctccgctcg catcacaaag ctgccgctgg
      721 tgttcaacgt cccgcccgta actttgtcat tcgccgattt ctccaccggc attggttcgc
      781 cggtcagcat ggattcgtca actgcgctgg cgccatccag cacaacacca tccaccggaa
      841 cgcgctcccc gggacgcacg cgcaagcgat caccggtttg cacttggtcc agcagcacat
      901 cgctttcggc ggaattcttg tctaagcggt gcgcgacttg cggcgccaaa tccagcaggg
      961 cgcgaattgc ggacgaagtt ttcgctcgcg ctcgaagttc cagcacctgc cccagcaaaa
     1021 ccagcgtggt gatgaccgct gcggcttcga aatagacagg aaccgaacca ttcatttcgc
     1081 ggaaggatgc gggaaatatc cgcgggaaaa ccgtggcaac cgcgctgtcc aggaatgccg
     1141 cgccggttcc gagcccgatt agcgtgaaca tattggggct gcggttcacc agcgatgccc
     1201 agaaacgttc aaaaaatggc cagccgcccc acataacgac gggcgaggcg agcgcaaact
     1261 ggatgccatg ccgtgctgag ccggaaagcg gcagcgcgat gtgctccccg aacattgcaa
     1321 gcagcactac cggaaggctg agcgccgcac tgacccaaaa tcgcttgcgc atggaaaggt
     1381 attcgggatc ggcagcttcg ctggaatcat tcggcaggaa gtccatcggc tccagcgcca
     1441 tgccgcactc cggacacgtt cccgcagcaa actgaattac ttcgggatgc atcgggcagg
     1501 tgtagcgaat cttgttctgg gcctgagcgt gcgccggcgc cgctgcatga tgaatgggaa
     1561 acggagacgg cggcgcctgg ggcgttacta tgcgcagcaa atacgtttcc ggatttgcgg
     1621 aaaactcttt cgcacagccg ggcgcgcaga aatagtacgc cttgccgccg tgttccactt
     1681 gcgcagcagc cttggccggg tcaaccgtca ttccgcagac cggatctcgt tccatttttc
     1741 ctcttcatct ccctcgagcg cgcctgctca ccgcaaatgg ctgtagatca atcccagcag
     1801 ttcgtcgtac atcgcgtcgg cttgttcttt tttcgccgaa cgcagcgctt ttgcggcgca
     1861 gtggtggaga tgatttttca tcagattgcg cgccacaccg cgcagcgctt cctgggcggc
     1921 ggcgatttgc gtaatgatgt cggcgcagta gcggtcgtct tcgaccatct tttgcagccc
     1981 gcggatttgc ccttcgatcc ggcgcagtcg tttgcgattg ctttctttca gcgccggatc
     2041 aaccgccaga gctttgtgac ccggcccgat gcggcaggca cagtcagcgc cgtgtgccgt
     2101 cgtttctgac ttcgaattgg aagtgtgtgc catacgccat ataccctata ggggtattta
     2161 tacccccggc tcctatcttg tgtcaaatcg tcgcggcccg gcttttctat tccgcccaga
     2221 caggcctgct atactaccgg catgacgctc ctcgaaatcc gctacgcact ccagtctccg
     2281 ctcaatgccg agcagttgcg caatctgagc gaattcgcga acacgtatgg ccttcgcaaa
     2341 ttcaagctca atgaggctaa gacggaattg tcgttcgaat atgatgcttc gcgcttgcgc
     2401 gagacgcagg tggcacacgc actgggtttg gcaaagattg cggtagttaa gaagttgaat
     2461 tgagggggtc agccacgatc ggccagcttg acctcccgat ttcgactcgt aacaatcgag
     2521 gaggccgctt aatggcagcc cagacaaccc gacggcggat cgggcccacg cccgaggcaa
     2581 gaagatagag cccccgcgcc gggaaccaac ccaaggcctg cgccggggca atccaactta
     2641 ttgattctta agttgttata actctacaac ttcgcccagc acaagtcccg ttctgcttcc
     2701 aaaaagccag actggaactg gcgtactttc aagagtggat
//
LOCUS       DASTPM010000743         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1065387, whole genome shotgun sequence.
ACCESSION   DASTPM010000743 DASTPM010000000
VERSION     DASTPM010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065387"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFR42_13125"
     CDS             complement(<1..272)
                     /locus_tag="VFR42_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370152.1"
                     /translation="MPNPLVSKVLSGKWTKVVLFLVCLAPLAAIIWPFARGYALANPL
                     EFIQHGTGDWTLRLLLITLTITPLRQLLHLPELIRFRRTLGLFAFF"
     gene            complement(287..1249)
                     /gene="msrP"
                     /locus_tag="VFR42_13130"
     CDS             complement(287..1249)
                     /gene="msrP"
                     /locus_tag="VFR42_13130"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEU0370153.1"
                     /translation="MATQKSSDLTYADVTPKSVWMDRRKFLRGAGIAGIAAFAGKRLY
                     DYATPSRSVQAGTKLTTIKSPFSTSEKINTFDVVTNYNNFYEFGVDKDQPAKNAQKFV
                     TASWTVAVSGEVAKPRKFSMDEILKLAPLEERIYRHRCVEGWSIVVPWIGFSFSTLAK
                     LVEPTSKARFVAFESYYDPRQMPLGRVAGIALPYVEGLRLDEAMHPLTLLCVGMYGET
                     LPNQDGAPVRMIIPWKYGFKSIKSLVKIRFVKDQPDTTWNLYSSHEYGFYSNVNPEVD
                     HPRWSQSHERRLPEFFKSHKTLMFNGYGDQVASLYTGMDLRKNY"
     gene            1502..1975
                     /locus_tag="VFR42_13135"
     CDS             1502..1975
                     /locus_tag="VFR42_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0370154.1"
                     /translation="MTNFFVNGKPVQVDVVDPSTPLLWVLRDSLGLTGTKFGCGMALC
                     GACTVHLDGKAIRSCVAPVSRAEGKHVTTIEGLSPDLTHPLQKAWIELDVPQCGYCQS
                     GQIMSAAVLLKEVHKPTDKDIDEAMGGNICRCGTYQRIRQAIHRAAELSDGGRTR"
BASE COUNT          466 a          552 c          660 g          425 t
ORIGIN      
        1 tagaaaaaag caaacagccc cagcgtgcgg cggaagcgaa tcaactcagg taagtgaaga
       61 agttggcgca gcggggtgat cgtgagggtg attaggagaa gacgcaaagt ccagtcgccg
      121 gtgccgtgct ggatgaattc cagcggattg gccagtgcat atccacgagc aaaaggccag
      181 atgatggcgg caagcggcgc gaggcaaacg agaaagagaa ctactttggt ccacttgccg
      241 gagagaacct tgctgacgag agggtttggc acgcaataat ttccgactag tagttcttgc
      301 gcagatccat gccggtgtag aggctggcga cctggtcgcc gtagccgttg aacatcagag
      361 tcttgtgcga cttgaagaat tccggcaggc gacgttcatg agactggctc cagcgcggat
      421 gatcgacttc gggattcaca ttggaataga agccgtactc gtgcgaggaa tagaggttcc
      481 acgtggtatc cggctgatct ttgacgaagc ggatcttgac cagggacttg atgctcttga
      541 acccatattt ccaggggatg atcatgcgca ccggggcgcc gtcctgattg ggcagagttt
      601 cgccgtacat tccgacgcaa agcagggtca acgggtgcat ggcttcgtca agacgcaagc
      661 cttcgacata gggcagcgcg attccggcga cgcggcccaa cggcatctgg cgcggatcgt
      721 aataactttc aaaagcgaca aagcgggcct ttgacgtcgg ctcaacgagc ttcgccaatg
      781 tgctaaagga aaacccgatc cagggaacga cgatagacca gccctcgacg cagcgatgac
      841 ggtaaatgcg ctcttcgagc ggcgccagct tgaggatttc gtccatcgag aacttgcgcg
      901 gcttggcaac ttcgccgctg acggcaacgg tccaggacgc ggtcacaaat ttctgcgcgt
      961 tcttggccgg ctggtcttta tccacgccga attcgtagaa attgttgtaa ttcgtaacga
     1021 catcgaaggt gttgatcttt tcgctcgtgc tgaaggggct tttaatggta gtgagtttgg
     1081 tgccggcttg tacgcttcgc gaaggagtcg cgtagtcgta aagacgcttg ccggcgaacg
     1141 cggcgatacc agcaatgccg gcgccgcgca ggaatttgcg acgatccatc cagacggact
     1201 tcggtgttac gtcggcatac gtcagatcac ttgatttttg cgtggccatg cctgcctttc
     1261 ggttcgcgaa ccctgccccc tcttattatg ctacgtttgg catccgggat tggatggcca
     1321 agccccgaaa cacccgggaa agatacgacg cgcgggcgaa ggcaaaattc cgctccaaac
     1381 gccgaggttt ctacactaaa ctccgcatcc aagcccggcg cgaccggcgt aactttgttt
     1441 tgccgctcgt catccaagtg gaaaccacgg ttgccgttag caaaatacgg gggaaacctt
     1501 catgacgaac ttcttcgtaa acgggaagcc tgtccaggtg gatgttgttg atccgtccac
     1561 tcctttgctg tgggtgctgc gcgactccct aggactaacc ggaacaaagt ttggttgcgg
     1621 gatggcgctc tgcggagcgt gcaccgtgca cctggatgga aaggcgatcc ggtcgtgtgt
     1681 ggcgccggtt tcgcgcgccg aagggaagca tgtaacaacg attgaggggc tctcgcccga
     1741 tttgacgcac ccgctgcaga aagcatggat cgagttggac gtgccgcaat gcgggtattg
     1801 ccagtcggga cagatcatga gcgccgcggt gctgctgaaa gaagtgcaca agcccacgga
     1861 caaagatatt gatgaggcta tgggcgggaa tatctgccgt tgcgggacgt accagcgtat
     1921 tcgccaggcg attcaccggg ccgcggaact cagcgacgga gggaggacgc gatgagcgcc
     1981 cccgcgatgg tcgggcggcg agaatttctc aagaccggcg cggccgtggg cggcgggctc
     2041 ttggtgagct ggcatatgcc gttgagcgca gcgggcatgc gcacatcgac agctcccgac
     2101 aac
//
LOCUS       DASTPM010000744         4533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_344360, whole genome shotgun sequence.
ACCESSION   DASTPM010000744 DASTPM010000000
VERSION     DASTPM010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344360"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFR42_13140"
     CDS             complement(<1..770)
                     /locus_tag="VFR42_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0370155.1"
                     /translation="MSILFELRYPTKWYTKLFTAVVAVVFFAGLATLAIAGFLVYRIV
                     KPNRTTSEINLQSFPGRPESVQFEVPGPNGHREGWFFPGFRGAPTIVLCHGYESSKNE
                     LLTLVSALQEHQYNVFVFDFVAHGANPGTTAFGYRETVELKAAMDAVGQRSDVDPDSF
                     GVWGYNLGAYVAMREAESDKRVKALVLDSIYDEPKEMVKVEVAKTGLGGFPFMVRSAE
                     MSFDWLNYEHRQDPPLSAKMTTLNGVPKLFLQPADDQQL"
     gene            1007..1939
                     /locus_tag="VFR42_13145"
     CDS             1007..1939
                     /locus_tag="VFR42_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0370156.1"
                     /translation="MNIVDLRQTTVRQIEPLLEEESLHWRDELHWDYRGALELIKRFL
                     EAHALSGCVAYENGSAAGYTFYVMEESKGLIGGLYVSPQFDQAAIARRLLEEMLYGMR
                     TLPNLSRIEAQLMPFGGPVDNPLRELGFRLYTRQFMLLDLTKPLDMSPGATTGLRISR
                     WHDRYFDPCAKLIFLAYANHIDGDINDQYRSKSGALRFLKNIILLPGCGQFVPGASFV
                     LHMPGSDELLGAVLTSEVSPGVGHTTQLCVIPGHQGHGLGRALMVTAVDALRGLKFHE
                     LTLTVTSVNHSAVQLYEKLGFKTLKSFTAGVWPR"
     gene            complement(1940..2404)
                     /gene="nrdR"
                     /locus_tag="VFR42_13150"
     CDS             complement(1940..2404)
                     /gene="nrdR"
                     /locus_tag="VFR42_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU0370157.1"
                     /translation="MKCPFCGHLDDKVIDSREGRAGDMIRRRRECLKCERRFTTYERI
                     DEIPYMVIKKDGRRERFERQKILQGLLKACEKRPVATPKLEAIVDEIEGVVQEATERE
                     LTTTEIGEMIMHRLKKLDKVAYVRFASVYMDFKDVQEFMAELKNLLKDRAKR"
     gene            complement(2410..3528)
                     /locus_tag="VFR42_13155"
     CDS             complement(2410..3528)
                     /locus_tag="VFR42_13155"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU0370158.1"
                     /translation="MTSRSKKWIFFAILVLIAGYLIYRSSGSLGLSGFSAAKLWGAIR
                     SANPFWMVAGMLLIYCCYALRSLRWQVFQRNLGRAKFWEIYPATLAGFSAVFLLGRAG
                     EPIRPLLLAKRAKHPVADIFGVWVLERLFDVASMAVIAAIALLVFKGNAHPGEAGETI
                     AKAAQTAGTLLAVGVGSAIVFLLYLRLHGTALLEPRLQRWRGSSSWRSRIAKIVLGFI
                     TGIQTVRSAADLFWSVIYSVVHWFLVLVVYYCVAQSFGGRLAELRLSDCMLVLAFTLV
                     GSAVQLPAVGGGSQALAIFAFTKVFGVDAETAVAAAVVLWLVTFAGCAIAGIPLLIRE
                     GVSLGQLRELAQHEKEELKEIAAHGPAHEKVPADGQGE"
     gene            3767..4210
                     /locus_tag="VFR42_13160"
     CDS             3767..4210
                     /locus_tag="VFR42_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370159.1"
                     /translation="MTLTNTQLSRREFARRAALGTAATSAMLLPLSDLAAAGPSSSMA
                     QLPADASKLSPQSQAEAEARYQSILALYAERLSDAQKTDLKRLCFALQPQLDRLRAWP
                     IANSAQPALYLKPLVEREKKSDAAPNLKSAPTPTSPVKSLAPRKP"
     gene            4217..>4533
                     /locus_tag="VFR42_13165"
     CDS             4217..>4533
                     /locus_tag="VFR42_13165"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0370160.1"
                     /translation="MVSSDILYLSASELGKRIQSKTLSPVELTKAYLDRSEKLGSKFN
                     AYARLTPEVALEQATAAEKEIGRGHYRGPLHGIPYAAKDLLATKGITTSWGAKPYENQ
                     IFD"
BASE COUNT          996 a         1353 c         1246 g          938 t
ORIGIN      
        1 agctgctgat catctgccgg ctgcagaaat agttttggca cgccgtttaa ggtcgtcatt
       61 tttgcagaaa gcggcggatc ctggcgatgc tcataattga gccaatcgaa gctcatttcc
      121 gccgagcgaa ccataaacgg aaacccgccc agaccggttt tagccacctc gactttcacc
      181 atttctttcg gttcgtcgta gatggaatcg agcaccaggg ctttcacccg tttgtcgctt
      241 tccgcttcgc gcatcgccac atacgcaccg aggttgtagc cccacacgcc aaagctgtcc
      301 ggatcgacgt ccgagcgctg gccaacggcg tccatagccg ctttcagttc caccgtctcg
      361 cgatagccaa acgccgtcgt gccaggattg gcgccgtgcg ccacaaaatc aaacacgaac
      421 acgttgtact ggtgctcctg caacgcagaa accaaggtga gcagttcgtt tttgctggac
      481 tcatagccgt ggcagagaac aatcgtgggt gcgccgcgaa atcccgggaa aaaccagccc
      541 tcgcggtggc cattcggccc gggaacttcg aactggacag attcgggacg gcctggaaaa
      601 ctctgcagat taatctcgga agtagtgcgg ttcggtttga cgatgcgata gaccagaaag
      661 ccggcgatcg ccagcgtcgc caatcctgca aagaaaacca cggcgacgac cgccgtgaat
      721 aacttcgtgt accacttcgt tgggtagcgg agctcgaaga gaatcgacat acgtagcctg
      781 cgcgactggg cttgcggcgc gagccgctat ccgttgtctc aacctcgttg gggagatagt
      841 ttccggtccg aactgactct agcacgctcg ggaaattgcg aaagctcgac gcggtacagg
      901 tgtgtcgcat cgatatcggg gacagcctcc atccgagtta ccggactgcc atttgcgaat
      961 cggctatgat agaaaggcgc agggaacgcg ccggccttta cccttcatga acattgttga
     1021 cttgcgccag accactgtcc gccagattga gccgctcctg gaagaagaat ccctccattg
     1081 gcgcgatgaa cttcattggg actatcgcgg cgctctggaa ctgattaaac gctttctgga
     1141 ggcgcacgcg ctatctggat gcgtggctta cgagaacggc tccgccgcgg ggtatacgtt
     1201 ttacgtgatg gaggagtcga aagggctcat cggcgggctg tatgtttccc cgcagttcga
     1261 tcaggcggcc attgcgcgcc ggctgttgga ggaaatgttg tatgggatgc ggacgctgcc
     1321 caacctctcg cgaattgaag cgcagctcat gccgttcggc ggtccggtgg acaacccgtt
     1381 gcgcgaactg gggttccggc tgtatacccg gcagttcatg ctcctggacc tgaccaagcc
     1441 gctggacatg tcaccgggtg ccacgaccgg actgcgaatt tcgcgctggc atgatcgcta
     1501 ctttgatccg tgcgccaaac tgattttttt ggcctatgcc aatcacattg acggcgatat
     1561 caacgaccaa taccgcagca agtccggcgc tctgcgtttt ctaaagaata ttattttgtt
     1621 gccgggatgc ggccagtttg ttccgggcgc ttcgtttgtg ctgcacatgc cgggcagtga
     1681 tgaattgctc ggcgcagtcc tgacgagcga agtctcgccc ggcgtcggac acaccactca
     1741 gctttgcgtg atcccgggcc atcaaggcca cggacttggt cgtgctctga tggtgacggc
     1801 ggtggatgcg ctgcgcggac taaagttcca cgaactcacg ttgacggtca cctccgtcaa
     1861 tcacagcgcc gtgcagctct acgaaaaact cggcttcaag acgctgaaat cctttaccgc
     1921 aggcgtctgg ccccgttagt tacctctttg cgcggtcttt gagcaggttt ttcagctcgg
     1981 ccataaactc ctggacgtcc ttaaagtcca tgtaaacgct ggcgaagcgg acgtaagcaa
     2041 ctttgtccag ctttttaagg cggtgcatga tcatttcacc gatttcagtg gtggtgagtt
     2101 cgcgttcggt ggcttcctgg accacgcctt caatttcgtc cacaatggcc tccagctttg
     2161 gagtagccac cggccgcttt tcgcaagctt tcaataggcc ttgcagaatt ttctggcgct
     2221 cgaagcgttc gcggcgccca tctttcttga tgaccatgta ggggatttcg tcaatccgct
     2281 cgtaggtggt gaagcggcgc tcgcacttga ggcattcgcg ccgccggcga atcatgtcac
     2341 cagcgcggcc ctcgcgcgaa tcaatcacct tgtcgtccag atgcccgcag aatggacact
     2401 tcactcggct tattctccct ggccatccgc cggaaccttc tcgtgagcag gtccgtgcgc
     2461 ggcaatttcc ttgagctctt ctttttcgtg ctgggccagt tctcgcaact gtccgagcga
     2521 aacgccttca cgaattaaca gtggaatacc cgcgatagcg caaccagcga aggtcaccag
     2581 ccacagcacg actgcggcgg ctaccgcggt ctccgcgtcc acgccgaaca ccttcgtgaa
     2641 ggcaaatatc gccagcgcct gcgacccgcc gccaaccgcc ggcaactgta ccgcagaacc
     2701 aaccagcgtg aaggctaaaa caagcatgca gtcgctcagc cgaagctcag caaggcgccc
     2761 accaaaactt tgcgcaacac agtaatacac caccaagacg agaaaccagt gaaccacaga
     2821 ataaatgact gaccagaata gatccgcagc ggagcgtacc gtctgaattc cagttatgaa
     2881 cccgagcaca atcttggcaa tcctggagcg ccagctcgag cttccgcgcc acctttgcaa
     2941 ccgcggttcc aacaaagcgg taccgtgcaa gcgcaggtaa agcaaaaaaa cgatggcgct
     3001 gccaacgcct accgcgagca acgttccggc ggtttgcgcc gctttggcga ttgtttcccc
     3061 agcctcgcca ggatgtgcgt tccccttgaa caccaggaga gcaatggcgg cgatgacggc
     3121 catgctggcg acatcgaaca ggcgttcgag aacccagacg ccgaaaatgt cagccactgg
     3181 atgcttggcg cgtttggcca acagtaacgg ccggatgggc tcgcccgccc ggcccagcag
     3241 aaacaccgcc gagaagccgg ccagcgtggc gggataaatt tcccagaatt tagcgcgccc
     3301 aaggttgcgt tgaaacactt gccagcgcag ggagcgaagc gcgtaacaac agtagatgag
     3361 gagcattccg gcgaccatcc agaatgggtt ggcgctgcgg atggcacccc agagtttggc
     3421 tgcgctgaat cccgaaagac caagtgagcc gctggaccga taaatcaaat acccggcgat
     3481 caggacgaga atggcgaaga agatccattt tttggacctg gaagtcatgc cgaatgagcc
     3541 tactggcgac ggacagatgc gtcaagccag gtgcgtcaag cgaggtgccg tagtgcgatt
     3601 tgcaaagcac gcgtaggtac agggttgctt gcttagttgt actaaccggg cgcgatacgc
     3661 gagaacaaag gcctcgacat gacgtgcgag aaatgagttt gatgcgctgg attcactgct
     3721 gtgggttaag attccgtagt tcaatccctt ggatgaacgc ccaaacatga cacttactaa
     3781 cacacagctc tcgcggcgcg aatttgcacg ccgggcggct ctcggcacgg ccgctacatc
     3841 tgcgatgcta cttcccttgt cggacttggc cgccgccggc cccagttcgt cgatggcgca
     3901 gcttcctgct gacgcttcca agctttctcc gcaaagtcag gctgaggcgg aagcccgtta
     3961 ccaatccatt cttgccctgt acgccgagcg cctttccgac gcgcagaaaa cggatctgaa
     4021 acgcctctgc ttcgcgcttc agccgcagct cgaccgtttg cgagcttggc caattgctaa
     4081 ttccgctcag cccgcgttgt atctgaagcc gctggtggag cgcgagaaaa aatccgacgc
     4141 ggctccgaat ctcaaatccg ctcctacgcc cacttcaccc gtcaaatcct tagcgccaag
     4201 gaagccctga gccgccatgg tctcgtccga catcctctat ttgagcgctt cggaacttgg
     4261 caaacgcatt cagtccaaga cgctctctcc tgtggagttg accaaggcgt acctcgaccg
     4321 ttccgaaaaa cttggctcaa aattcaacgc ctatgcccgc ctcactccgg aagtcgcgct
     4381 cgaacaggcg acggcagctg aaaaggaaat tggccgcggc cactaccgcg gtccgttgca
     4441 cggaattccc tacgcggcaa aagatttgct cgcaacaaaa ggaatcacta ccagctgggg
     4501 cgccaagcct tacgaaaatc agattttcga cta
//
LOCUS       DASTPM010000745         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1295544, whole genome shotgun sequence.
ACCESSION   DASTPM010000745 DASTPM010000000
VERSION     DASTPM010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295544"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFR42_13170"
     CDS             complement(<1..656)
                     /locus_tag="VFR42_13170"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEU0370161.1"
                     /translation="MKLRCVAVLAALLCGGVPTRLCGQQTAPLVPAADQAAPPVTEFR
                     VVTSEGRVLRVAGNPISIEIGKPLDPAQVASSLKALYRLGNFSDIRAVSQQAAGGVRI
                     DFIVQENLFFNQVILRGLKPPPTEASAAAAMQITLGDVFRKETLDDGLERLRTRMQEE
                     GFYHAKLDAQLLPHPAEHQMDIIVHVQPGVRARVGTVDLTNNTEYPDSEIISRLKMKP
                     G"
     gene            783..1844
                     /locus_tag="VFR42_13175"
     CDS             783..1844
                     /locus_tag="VFR42_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEU0370162.1"
                     /translation="MTENAKAGNHPSTNPAWERYSRQILFAGMGEQGQRKLLESSAVV
                     VGCGAIGASTAQLLARAGVGRVRVIDRDFVEPSNLQRQALFDENDASEALPKAVAAER
                     KLRLLNSQIQVEGAVADVMPRNIEELLDGFDLILDGTDNFETRFLLNDYAVQTARPWI
                     YAAAVGSYCVMFAIRPSQTACLACLMETPDAGPLLEETCDTVGVLGPAVNLVSSLEAA
                     EALKFLSGNTAALHRRLISCDVWSGRMQSVAVARNITCRACGRREFSYLEGEAQPHIT
                     MCGRDSVQIHERRRALDLAALQANLAAAGLAVKQNDFLLKFSVPPYEMTVFGDGRAIL
                     KGSTDPAVARSLYARYIGA"
     gene            complement(1866..2213)
                     /locus_tag="VFR42_13180"
     CDS             complement(1866..2213)
                     /locus_tag="VFR42_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370163.1"
                     /translation="MPTSCHIAGCKGTAPHALEEQKLCVLHYTLSLESSCSEMRRETA
                     LGNAPHDRQREIMRFITESGEKLARVATSGMALTDDLKARILSTFLTLMNLRENLDRA
                     SMRTPPGRSGVFR"
     gene            2398..2871
                     /locus_tag="VFR42_13185"
     CDS             2398..2871
                     /locus_tag="VFR42_13185"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370164.1"
                     /translation="MANAKTFRHGQILRLVTAERIANQEELRRRLSGQRLRVTQATLS
                     RDLQELKLVKTPAGYRPATALVEEIAPMPPLARALGEFLLDIRPAENLLVLKTPPSGA
                     QPLAAAVDGAKFPEIAGTIAGDDTVLIITPNKKQRASLQQKIESFVSRPLRSRAS"
     gene            2873..3904
                     /gene="argC"
                     /locus_tag="VFR42_13190"
     CDS             2873..3904
                     /gene="argC"
                     /locus_tag="VFR42_13190"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU0370165.1"
                     /translation="MKNPENIAVVGVSGYTGLELAKILLRHPEAEKLTFYVREPQGAH
                     CLAEMFPQLRGWGQAPVKALSVEAIAESGAGTAFLATPHEASAELAPKLVEAGMRVVD
                     LSGAFRFREAETFARWYKLPTPHAGWLGQAVYGLPEFYAKELAQAKLVANPGCYATSV
                     ILAVRPLAEAGWIAGGTSVVCDCKSGATGAGKELRRDLQFSELDENFKAYNLFAHRHT
                     PEIIEHTSLPETQVIFTTHLLPLARGILSTIYVTLKEPQTADAIEALYRKAYAGRPMV
                     RICKAGSLPELQHVAHTNFCDIGFALDGSGRRLIVVSCLDNLGKGAAGQAVQNFNHLL
                     GIEEQTGLQ"
     gene            3901..4746
                     /gene="argB"
                     /locus_tag="VFR42_13195"
     CDS             3901..4746
                     /gene="argB"
                     /locus_tag="VFR42_13195"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU0370166.1"
                     /translation="MKIVIKFAGVLLEDAQTVKSLAGQVAALAKRGHEILVVHGGGRV
                     FTATLKRMGIESKFVAGLRVTDRETREVALMVFAGLLNKQLAAAITAAGQPAIGISAA
                     DAESFTAEPLVHNDVPGSLGYVGYLTGANTDLLASLWNAGLVPVAACLGLGPDGELYN
                     INADHMAAACAEYLQADQLIYLTDVHGVLDGERTLSALACDEMERLVQSKVVSGGMVL
                     KLEAAKRALEGGVRDVHIVGGTLPDGLLAAVRAAGDGPSINLPGTRVLREPISLSEPA
                     AFSAA"
     gene            4746..>5176
                     /locus_tag="VFR42_13200"
     CDS             4746..>5176
                     /locus_tag="VFR42_13200"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0370167.1"
                     /translation="MSKFKKNRLGPSISRGLRRQKDGKLTEAIAMAEKYLLPTYKRPA
                     IVFTHGRSAYVYDSAGKKYLDFLGGIAVNALGHAHPRIVRVIRREAGHAIHLSNLFHH
                     KFQGRLAKKLSEWSGLDRVFFANSGTEAIDGALKLARLCGRP"
BASE COUNT         1054 a         1292 c         1815 g         1015 t
ORIGIN      
        1 ccgggcttca tcttcaggcg ggaaatgatt tcgctgtcgg gatattcggt gttgttcgtc
       61 aaatcgacgg tgccgacgcg cgcgcgaacg ccgggctgta cgtggacgat gatgtccatt
      121 tggtgctcgg caggatgagg caatagttgg gcgtcaagtt tggcatggta aaagccttct
      181 tcctgcatac gagtgcgcag gcgttcgagc ccatcgtcca gagtctcttt gcggaaaaca
      241 tcgccgagcg tgatttgcat ggcggcagcg gcggaagctt cggtgggagg aggtttaagt
      301 ccccgcagga tcacctgatt gaagaagaga ttctcttgca cgatgaaatc aatgcgaact
      361 ccgccggcag cctgttgcga aacggcgcgg atatcagaga aattccccag gcgatacagc
      421 gcttttagac tggatgcaac ttgcgccgga tcgagcggct tgccgatttc gatggagatg
      481 ggattcccgg cgacgcgaag aacccggccc tcggatgtga ctacacgaaa ttcagtaaca
      541 ggaggcgcgg cctgatccgc ggcgggaaca aggggagcag tttgttgtcc gcaaagacgg
      601 gtgggcactc ctccgcagag gagcgccgcg agcaccgcga cgcaccgcaa tttcacacca
      661 ttcgccctca tgcccccaat ttgctcagac gaagggtagc acaggggcgt tgcctgcttc
      721 cctatctgac ggcgagaggc gcgcaacctg aaacaaagga gcattggagc tatcatcgtg
      781 aggtgaccga aaacgcgaaa gcgggtaatc accccagtac aaacccggcc tgggagcgat
      841 attctcggca aattctgttt gccgggatgg gcgaacaggg ccagcggaaa ttgctggagt
      901 ccagtgccgt ggtggtgggc tgcggggcga ttggagcttc cacggcgcag ttgctggccc
      961 gagcgggagt aggacgcgtg cgagtgattg atcgcgactt tgtggagccg tcgaatcttc
     1021 agcggcaggc gctgtttgat gaaaacgatg caagcgaagc tctgcccaaa gcagtggcag
     1081 cggagcgcaa gctgcgtctg ctgaactcac agattcaggt ggagggcgcg gttgccgacg
     1141 tgatgccgcg caacattgag gaattgctgg atggcttcga cctgattctg gatggaacgg
     1201 acaacttcga gacgcggttc ttgctgaacg attatgcggt gcaaacggcg cgtccttgga
     1261 tttatgcggc ggcggtgggc agctactgcg tcatgtttgc gatacggccg agccagacgg
     1321 catgtctggc ttgtttgatg gaaacgcccg atgccgggcc gctgctggaa gaaacgtgcg
     1381 acacggttgg agtgcttggg ccggcggtga atctggtgtc ctcgctggag gcagcggagg
     1441 cactgaagtt tttgtcgggg aatacggcgg cgttgcatcg ccggctgatt tcctgcgatg
     1501 tgtggagcgg gcgaatgcag tcggtggctg tcgcgcggaa tataacttgc cgagcgtgtg
     1561 ggcggcggga attttcttat ctcgaaggcg aagcacaacc gcatatcacc atgtgtggac
     1621 gggattcagt gcagatccac gagcggcgac gggcactgga cctggcggcg ctgcaagcga
     1681 acttggccgc ggcgggattg gcggtgaagc aaaatgattt cctgctgaaa ttcagcgtgc
     1741 cgccgtatga gatgacggtt ttcggtgacg ggcgggcgat tctgaaggga tcgacagatc
     1801 cggcggtggc gcggtcactg tatgcgcggt atatcggggc gtgagacgcc gctgggcgaa
     1861 agtggctagc ggaagacgcc gctgcggccg ggcggcgtgc gcatagaggc gcggtcgagg
     1921 ttttcgcgca gattcatcaa cgtcaaaaat gtgctgagga tgcgggcttt caggtcgtcg
     1981 gtgagcgcca tgccactggt cgctacgcgc gccagctttt cgccgctttc ggtgatgaag
     2041 cgcatgattt cgcgctgacg gtcgtgcgga gcattgccga gcgcggtttc gcggcgcatt
     2101 tcggagcaac tggattccaa cgacaacgtg tagtgcagaa cgcaaagctt ctgctcttcg
     2161 agggcatgag gcgcggtgcc cttgcagcca gcaatgtggc aggacgtagg catgttcgat
     2221 gactataaat aacgttagca acagagcgcg cggaaaagcc agcgccgtgc gcggcgatgt
     2281 gcgctggatg taagcagggg cgcggagaac gcgtgggacg caccgcaagc aaaaatccgt
     2341 tgacaagttt attcagaagc atgtattttt atacagcacc gtgaataacg gctgcatatg
     2401 gcgaacgcaa agacattccg gcacgggcag atcctgcggc tggttaccgc tgaacgcatt
     2461 gccaaccagg aggagttgcg gcggcgcttg tctgggcagc ggctgcgtgt gacgcaggcg
     2521 acactttcgc gggatttgca ggagctgaag ctggtgaaaa cgccggcagg ctaccggccg
     2581 gcgaccgcgc tggtggaaga aattgctccg atgccgccgt tggcgcgggc gttgggggag
     2641 tttttgctgg atattcgtcc cgcggagaat ttgctggtgc tgaagacgcc gccgagcgga
     2701 gcgcagccgt tggccgccgc cgtggacgga gcgaagtttc ccgaaatcgc cggaaccatt
     2761 gccggcgacg acaccgtgct gatcattacg cccaacaaaa agcagcgcgc gtcgctgcag
     2821 cagaagatcg aatcgttcgt gagccgcccg ttgcgcagcc gcgcttcata gaatgaaaaa
     2881 tccggaaaac attgcggtgg taggtgtctc cggctatacg ggcttggagc tggcaaaaat
     2941 actgttgcgc catccggaag cggagaaatt gacgttttac gtgcgcgagc cgcaaggagc
     3001 gcattgcttg gcggaaatgt ttccgcagct gcgcgggtgg ggtcaagcgc cggtgaaggc
     3061 tttgtcggtg gaagcgatcg cggaaagtgg agcggggacg gcatttttgg cgacccccca
     3121 tgaagcttcg gcggaactgg cgccgaagct ggtggaagcg ggaatgcgag tggtcgattt
     3181 aagcggagcg ttccggtttc gcgaggcgga gacgtttgcg cggtggtaca aactaccgac
     3241 gccgcatgca gggtggctcg gccaagcggt gtacggtctg ccggagtttt acgcgaagga
     3301 acttgcgcag gcgaagctgg tggcgaatcc gggctgttac gcgaccagcg tgattctggc
     3361 ggtgcggccg ttggcggaag cgggatggat cgccggtgga acgagcgtgg tgtgcgattg
     3421 caagtccggt gcaacgggtg cgggaaaaga attgcggcgc gatctgcaat tttccgagct
     3481 ggatgaaaac ttcaaggcgt acaacctgtt tgcgcaccga cacacgccgg aaattatcga
     3541 gcacaccagc ttgcctgaga cgcaagtaat ttttactacg catttgctgc cgctggcgcg
     3601 cggaattctt tcgacaattt acgtgacgtt gaaagagccg caaacggcgg atgctatcga
     3661 ggcgctttac cgcaaggctt atgcgggacg gccgatggtg cgcatctgca aggccggaag
     3721 tctgccggaa ctgcagcacg tggcgcatac gaatttttgc gacatcggat ttgcgctgga
     3781 cggaagcggg cggcggctga tcgtggtgtc gtgcctggac aatttgggca agggcgctgc
     3841 ggggcaggcg gtgcagaact tcaaccatct tttgggaatt gaggagcaaa cggggctgca
     3901 atgaaaatcg ttatcaagtt tgcgggtgtg ttgctggaag acgcgcagac ggtgaagtcg
     3961 ttggcgggac aggtagcggc gctggcgaaa cgaggccacg aaattctggt ggtgcatggc
     4021 ggcggacgag tattcacggc gacgttgaag cgcatgggca tcgagagcaa atttgtcgcg
     4081 ggattgcgcg tgaccgaccg tgagacgcgc gaagtggcgt tgatggtgtt cgccgggctg
     4141 ctgaacaaac aactggcggc ggcgattacg gcggcggggc aaccggccat cggaatttcc
     4201 gcggcggatg cggaaagttt caccgcggag ccgctggtgc acaacgatgt gccaggtagc
     4261 ctgggatacg tcggctatct gaccggcgcg aacacggatc tgctggcgtc gctgtggaat
     4321 gccgggctgg ttccggtggc ggcgtgtcta ggacttgggc cggacggcga actctacaac
     4381 atcaacgccg accacatggc tgcggcgtgt gcggaatatt tgcaggcgga ccagttgatc
     4441 tacctgaccg atgtgcacgg cgttttggac ggcgagcgaa ctttgtccgc cctggcttgc
     4501 gacgagatgg agcggttggt gcagagcaaa gtggtgtccg gaggaatggt gttgaaactg
     4561 gaggccgcga agcgggcgct ggaaggcggc gtgcgcgacg tacacatcgt cggcggtacg
     4621 ctgccggatg ggttgctggc ggcagtgcgt gcggcgggtg acgggccttc cataaacttg
     4681 ccgggaacgc gcgtgttgcg tgagccaatc agcctgagcg agccggcggc gttttcggcg
     4741 gcatgatgag caagttcaag aaaaaccggt tgggaccgtc gatttcgcgc ggcttgcgaa
     4801 gacagaagga cggaaagtta acggaagcga tcgctatggc ggaaaagtac ttgctgccga
     4861 cgtacaagcg gccggcaatc gtcttcaccc acgggcgcag cgcgtacgtt tacgattcag
     4921 ccgggaaaaa gtatctcgat tttcttggcg gcattgcggt gaacgcgctc ggccacgcgc
     4981 atccgcggat tgtgcgggtc attcggcgcg aagcgggaca cgcgattcac ctgtcaaatc
     5041 tgtttcacca caagtttcag gggcggctag ccaagaagtt gagcgaatgg tcgggtctcg
     5101 accgcgtctt ttttgcgaac agcggaacag aagcgattga tggcgcgttg aagcttgcca
     5161 gactgtgcgg gcgtcc
//
LOCUS       DASTPM010000746         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_401935, whole genome shotgun sequence.
ACCESSION   DASTPM010000746 DASTPM010000000
VERSION     DASTPM010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401935"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFR42_13205"
     CDS             <1..633
                     /locus_tag="VFR42_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEU0370168.1"
                     /translation="AETILPIELNLIAVPGATLDQIKTVSSHPMALAQCERFFTQHPL
                     FKRVPAEDTAGSVREVLGRGDKISAAIAGRRAADYYRGVILAEKIQDNSENFTRFVLL
                     VPQDSPSQDRLLSPRLDAKVHKVSLALRLAHQPGALLAALRPFADHGINLLKIESRPI
                     HGRPWEYQFFLDVQTSEPGKLHEALHQVRGATSFFRFLGSYAPASSESFS"
     gene            complement(997..1647)
                     /locus_tag="VFR42_13210"
     CDS             complement(997..1647)
                     /locus_tag="VFR42_13210"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0370169.1"
                     /translation="MATQVAVATELNEAKLIRAGQRGDAQAVETLFRRYQRPLFQTAL
                     RVLGNAEDAEDALQDGYLSAYRNLKRFEGRSQFSTWLTRIVINAALRRRRSAKSRPAV
                     SLDDSPREDELPASERFADDSPNPEQVYAGTELREMINQNLDELSPLLRTAFVLREVQ
                     GYSTGEAAKKLGVTENTLKARLWRARHQMAERLGRRLKRMKDDMTGGIGDPDCAYC"
     gene            complement(1700..>2791)
                     /locus_tag="VFR42_13215"
     CDS             complement(1700..>2791)
                     /locus_tag="VFR42_13215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0370170.1"
                     /translation="WITDNFSLRWIFYINVHVGIISLLLTSLLVSDPPYMKRTKVSEG
                     FRIDYIGIGLISLGLGSMQIILDKGEREDGLSSGFIQVFFVLMLIGIIAGIIWEMRHD
                     HPVVDLRMLKDRNFAVATLAMFFLGFVLYASTVLIPQFLQEMMGYTAELAGLALSPGG
                     AVIMFMMPVVGFLVSRVNTKYLITFGCLVSSLALFVMAGWNLQIDYKHAVLARMLQSF
                     GLAFLFIPINVSAFAFVPKEKSNMGTGIINLARNIGASVGIATVTTFLERRTQFHQAQ
                     LMEHVNSLSKTFQDRMHAMTASFTSGGSSGPGATSQAYGMIYNMVERQAVMKAFIDNF
                     YMLGFVFLAVIPVLMFLKRPPRGASAPVH"
BASE COUNT          590 a          827 c          785 g          589 t
ORIGIN      
        1 gccgagacaa ttcttcccat cgaactcaac ctcatcgccg tccccggcgc cacgctcgac
       61 cagatcaaaa ccgtctcgtc gcatcccatg gcgctggcgc aatgcgaacg cttctttact
      121 cagcatccgc tcttcaagcg cgtccccgcc gaagacaccg ccggcagcgt ccgcgaagtc
      181 ctcggccgcg gcgacaaaat ctccgccgcc atcgccggac gccgcgccgc cgattattac
      241 cgcggcgtca tcctcgccga aaaaatccag gacaactccg aaaatttcac gcgcttcgtg
      301 ttgctggtgc cgcaggattc gccgagccag gatcggctgc tgagcccgcg gctcgacgcc
      361 aaagtacaca aagtctcgct ggccttgcgg ctggcgcatc agccgggcgc gctgctggcg
      421 gctttgcggc ccttcgccga tcacggtatt aatctcctaa agattgagag tcgccctatt
      481 cacggccgcc cgtgggaata ccaattcttt ctcgatgtgc agaccagcga gccgggaaag
      541 ctgcatgaag cgctgcacca agtgcgcgga gcaacgagtt ttttccgttt tctgggaagc
      601 tacgctccgg ccagttctga atcgttttcc tgaaccagag tagtggccag tttgagtcgg
      661 ctgaaaattt tcaccgggta gagggcttgt ggaacctttt tttgcgagag gctagccgcc
      721 accagttggc tgcttaacga tgtgcgtcac gatctgattg gcgtcgcgtc tttgcggcgc
      781 ttgtttcgga gtcttcttct taggcttacg aatttcgcgt ttccttgcgt ttttgtttcc
      841 catagccagc atcatggcat attttcttga actaggaaat tctcaaattc agcaatgaac
      901 tttgactccg agcgttggcg cgcacaaggt gccgccggcc ttttcctttg gttcaaaacg
      961 gcagcggcct gcctgcgaca ggcaggccgc tgctcattag cagtaggcgc aatcggggtc
     1021 gccgattccg ccggtcatgt cgtccttcat gcgcttcaag cggcgcccca ggcgttcggc
     1081 catctggtga cgggcgcgcc acaagcgggc cttcagcgtg ttttcggtga cgccgagttt
     1141 cttggcggct tcaccggtag agtagccctg cacttcacgc agaacaaacg cggtgcgcag
     1201 gagcggtgaa agctcatcga gattctggtt gatcatttcg cgcaattccg tgccggcata
     1261 gacctgctcc ggattcggcg agtcgtcggc aaagcgctcg ctggccggca gctcgtcttc
     1321 gcgcggcgaa tcgtcgagcg aaacggctgg gcgagacttg gcgctgcggc ggcgcctcaa
     1381 agcggcgttg atgacgatgc gcgtcagcca ggtggaaaat tgcgaacgcc cctcaaagcg
     1441 cttcaagttg cggtaagcgg agaggtagcc gtcctgcaga gcgtcttcag cgtcttcggc
     1501 attgccgaga acacgcaacg cagtctggaa caagggccgc tggtagcggc gaaataatgt
     1561 ctcgaccgcc tgcgcgtcac cccgttgacc tgcgcggatc agcttcgctt cattcagctc
     1621 agttgccact gcgacttgtg ttgccatcgt ttcccccatt caactaagaa actcgaaccc
     1681 aaaacctctt attcccgccc tagtgcaccg gcgcgctggc ccccctgggc ggccgcttca
     1741 agaacatcaa caccgggatc acagcgagga agacgaaacc cagcatgtaa aagttgtcga
     1801 taaaggcctt cattaccgct tgccgctcga ccatgttgta gatcattccg taggcctgcg
     1861 aggtcgcgcc cgggccactg gatccgccag aagtgaagct ggcggtcatg gcgtgcatgc
     1921 ggtcctggaa cgttttgctc agcgagttga cgtgctccat cagctgcgct tgatggaact
     1981 gcgtgcggcg ctctaagaac gtagtcacgg tggcgatgcc gacgctggcg ccgatattgc
     2041 gcgccaaatt gatgatgccg gtacccatgt tgcttttttc cttgggcaca aaggcgaacg
     2101 ccgaaacgtt gatcgggata aacaagaacg ccagaccgaa actttgcaac atccgcgcca
     2161 ggaccgcgtg cttgtagtcg atctgcaggt tccatccggc catcacgaaa agcgccaatg
     2221 aagaaaccag gcagccgaag gtgatcaaat attttgtgtt gacacgagac acgagaaatc
     2281 ccactactgg catcatgaac atgatcaccg cgccgcccgg cgacaaggcg agcccagcga
     2341 gttcggctgt gtagcccatc atttcctgca ggaattgcgg gatcagcacg gtgctggcgt
     2401 acaggacaaa cccgaggaaa aacatggcca gcgtggcgac cgcgaagttc ctgtccttga
     2461 gcatgcgcaa gtcaacgacc ggatgatcat ggcgcatctc ccagatgatt ccggcgatga
     2521 ttccgattag catcaataca aagaacacct gaatgaagcc gctggagagc ccgtcttcgc
     2581 gctcgccttt gtcgagaatg atctgcatgc tgccaaggcc caggctgatc aagccgattc
     2641 cgatgtaatc gatgcggaat ccttcgctga ccttggtgcg cttcatgtac ggcggatcgc
     2701 tgacgagaag cgatgttagc aacaaagaga taatgccgac gtggacattg atataaaaaa
     2761 tccagcgcaa ggaaaaattg tcggtgatcc a
//
LOCUS       DASTPM010000747         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1007576, whole genome shotgun sequence.
ACCESSION   DASTPM010000747 DASTPM010000000
VERSION     DASTPM010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007576"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFR42_13220"
     CDS             complement(<1..727)
                     /locus_tag="VFR42_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0370171.1"
                     /translation="MAKILVADDNSNIQKMVGLALKDQGIDVVAVGNGEAAVRKISDV
                     RPDLVLADVFMPVRNGYEVCNYVKQDSTLSHIPVILLVGAFDPLDEQEAQRVGADGVL
                     KKPFVPPDPLISMVKSALTRAGVVIGAAEPPPPPPIAATKVEPPPLPRVAPPPPPPPM
                     AVPEPEIDLNVPVFSSRPAPLSIDKADQPLAFGSLLGSPVQEEDAVFTTSSGVPEAAP
                     ERDWGHATEQPEQEIAEDESKGAA"
     gene            complement(903..2345)
                     /gene="bamD"
                     /locus_tag="VFR42_13225"
     CDS             complement(903..2345)
                     /gene="bamD"
                     /locus_tag="VFR42_13225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU0370172.1"
                     /translation="MRRTLFVILGIAVIFSAAEAQAQTQTNTQEAPPTATLSNTGQNA
                     KKEAPAKKESKKSKKKKDAAPADDSVAPDKILYDRAIVDVKKGRHEVGRLNLQTLINT
                     YPDSEYLAKAKLAIADSYYKEGGSANWTQAIAGYKDFIVFFPFLPEAPYAQIQVAMTH
                     FKEMEKPDRDRTEAKAAEEEFQTFLSKYPKDPLVPKAEQHLRVVQEVLAEGDYRIGYY
                     YYVKGDRRAAAGRLLSVTKRYPLYSRSDQALWMLGDIFEKSEKKEIASVYYSRIVKDY
                     PLSALVPDAKKKLVTFGVPVPQPDPKQLAWMQAEAAAPREKPGVLSKPMSLIRTGPGK
                     EKMVAARTGTPNMEPETDTTSVADILSGGGKTQIGAGGSSSSPGNTAAVAIVTPGTGA
                     SGGSTVETGDTGATETAPAADANSSTAPATDAAPATTDGAAPATTGTDASASGAPADP
                     NAKPADNATTDPKKESSSKKKKGLKKIVPW"
BASE COUNT          527 a          894 c          796 g          681 t
ORIGIN      
        1 acgccgcgcc tttggattcg tcttccgcga tttcctgttc gggctgctcg gtggcgtgcc
       61 cccaatcgcg ctccggcgct gcttccggca ctcccgatga ggttgtgaat accgcgtcct
      121 cttcttgcac aggcgagccc agcaagctgc caaaggccag cggctgatcc gccttgtcaa
      181 tgctcagggg agccgggcgc gatgaaaata ccggaacatt caggtcgatc tctggttcag
      241 gtactgccat cggtggtggc ggtggaggag gcgcaactct tggcagcggc ggaggttcga
      301 ccttcgttgc cgcaatcggt ggcggcggag gcggctcagc cgctccaatc accactcccg
      361 cgcgcgtcag tgcagacttc accatcgaaa tcaatggatc gggcggcaca aacggcttct
      421 tcaacacgcc gtccgcgcca acccgctgtg cttcctgttc atccagcgga tcgaatgcgc
      481 ccaccagcaa aatcaccgga atatgagaaa gagtggaatc ctgcttcacg tagttacaga
      541 cttcgtagcc gttgcgtacc ggcatgaaca cgtccgccaa caccaggtcc ggccgcacat
      601 cggaaatctt gcgcaccgcc gcttcgccgt tgcccacggc cactacatca atgccctgat
      661 cttttagggc cagccccacc attttctgga tgttgctgtt gtcgtccgca accaggatct
      721 tggccaccga acttcctccg atgcacccac acacacgcgc tgctgcggct gctcatcagc
      781 cctacagcgt tattggagca tcgggggatg gaggcgtcaa cgcaaagtgc cccgggttgt
      841 gtcggaaggg aacacggccc tgtcgaacag gcaagattca ataccgtaag gcgcaggctt
      901 gcttaccagg gaacaatctt cttcaatccc ttttttttct tacttgagga ttccttcttc
      961 ggatcggtag ttgcgttgtc agccggcttg gcattcggat cagccggggc accgcttgcg
     1021 ctcgcatcgg tccctgtggt cgcgggtgcc gcgccatcgg tggtcgccgg cgcagcgtct
     1081 gttgcagggg cggtactgga attcgcgtcc gcagctggcg ccgtttccgt tgctccggtg
     1141 tctccagtct ccaccgtgga gccgcccgac gctcccgtcc ccggcgtgac gattgccacc
     1201 gccgcagtgt tgccgggcga gctgctgctt ccgcccgccc cgatttgcgt ttttccgcca
     1261 ccgctcaaaa tatccgcaac ggaggttgta tcggtctccg gctccatgtt aggagtcccg
     1321 gtccgcgctg cgaccatttt ttcttttccg gggcccgtgc ggatcagcga catcggcttg
     1381 ctgagcacac cgggcttctc gcgcggcgcc gccgcttccg cctgcatcca agccagttgc
     1441 ttgggatcag gctgcggcac cggtacgcca aacgtcacca gttttttctt cgcatccgga
     1501 accagcgcgg acagcggata atctttcaca atccttgagt aataaactga cgcgatctct
     1561 ttcttctcgc tcttttcgaa aatgtcgccc agcatccaca gcgcttggtc cgacctgctg
     1621 tacagcggat agcgcttggt caccgaaagc agccggccag ccgccgcgcg gcgatcgcct
     1681 ttcacgtaat agtagtagcc gatgcgatag tccccctcgg ccagcacttc ctgcaccacg
     1741 cgcaagtgct gctccgcctt cggcaccagc ggatccttcg gatatttcga caggaaagtc
     1801 tggaactctt cttccgccgc ctttgcttcc gtgcgatcgc ggtccggttt ttccatctcc
     1861 ttgaaatggg tcatcgccac ttgaatctgc gcgtacggcg cttccggcag gaaagggaag
     1921 aacacgatga agtctttgta cccggcgatg gcctgggtcc agttcgcgct cccgccttcc
     1981 ttatagtagg agtccgcaat cgctagcttg gccttggcca gatactcgct gtcgggatag
     2041 gtattgatca gcgtctgcag gtttaaccgg ccgacttcgt gccggccttt cttgacgtcc
     2101 acgatcgccc ggtcatacaa gatcttgtcc ggcgccaccg aatcatcggc cggtgcggca
     2161 tcctttttct ttttcgactt cttggattcc ttcttggcgg gagcttcctt cttggcgttc
     2221 tgcccggtat tgctcagtgt ggcggtggga ggggcttctt gcgtattggt ttgggtctga
     2281 gcttgggctt cggctgccga gaaaattacg gctatcccca gaatgacgaa cagagtgcgg
     2341 cgcaaacatc acctcgctag cgggaacttg ggtctcaggg ttcaagggtc aatttacaaa
     2401 cccttacaaa aacaagttgt acccgcaaaa cccgagtgca attgaaattg ttgcgcggcg
     2461 ccggactctc ggctgaagtt gagcttgttg tttatgccgc tggcaacaat ttcagtataa
     2521 cctgaaccaa aggacaggga agagaaactt tccccgccat ttggcgcttc accaagtccg
     2581 gcgaaagccc tgcttaggtt ctccattctg ctcagaatcc aaagctactg cccgggttgc
     2641 tcgccctttc gccgttttta atgttttgta acatcgattc gccggattgc ccttaacata
     2701 cttcgggagc cctgctcatc ctaaatggac aagcgcaaat ttcttttcgt ttcactctcc
     2761 ggtctgatcg gcgacatcgc ctgggaagtc ctcaaggaag gccacgatgt ccggtatttt
     2821 atcgaagcgc aaagcgagca ggacattgcc gatggcttcg ttcctaagtc aaaagactgg
     2881 gagcgcgacg cgaagtgg
//
LOCUS       DASTPM010000748         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_171157, whole genome shotgun sequence.
ACCESSION   DASTPM010000748 DASTPM010000000
VERSION     DASTPM010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171157"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            442..>2573
                     /locus_tag="VFR42_13230"
     CDS             442..>2573
                     /locus_tag="VFR42_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0370173.1"
                     /translation="MINLSLVSLALLIATSLVAGFFAYVYNLKRQTYLLCWTAGWCLL
                     ALHYLGTGLSGSLGPSAGQRAVDQALFCGAGILFFAGAKLYAQRRATLPVTIGAGAVV
                     LLWSIANAFGIIPLPVTFAAALIYGAVGFEFWVESQRQETLADWLLAVVFFAWGALGL
                     MFLFFQRSLSSWNIPFEPISALPATFAAMLMVMALYEEEKRRIERNMLALSNLNLAAS
                     GFAGGEIHRMLSQALDRVLGVVRLPVGALFLHHGDPQGPTSVVAVGLNEKFCRLAQEE
                     GLDDYLVNLVSSLGGLMGFRDLRDDDLMALEKKDQRIRRFRELALEQRLRSMVAISLQ
                     AKEQAFGVLLLGSPDTRRFAPAELRLLLALGHQIGMAVENSLLIQQTSRRSEELHVLN
                     EIGRALSSTLNKEDLLRKVWEELRRLFDVENFYIASLDPIGDEMQFELEIIDGIRMPR
                     RSRPAGNFLTEYVIRTRQPVLIRENFLDEVRKLNVDPIRVQGCFCGVPLVAYDRAIGA
                     MAVFSDQDKTFDEGHLELLRVLASEASIAIENARLFQEERTKARHLTLLNTISRHAIG
                     TLNPDEMLAKMTEELETGLTYDHIGIAVLDYSTRELVIQAEGGKRRGALGQRVPLGSG
                     LIGNVARAGNLVTFRSSLAADAAMRPMLADSVAAVGLPIFFAEQLHGVLYVESSENVD
                     FTEEEILLLGTLADLIAGALHNALTIQKA"
BASE COUNT          478 a          796 c          717 g          582 t
ORIGIN      
        1 gcagtgcgcg cgcccccgac gtggagatag ccggtgggag aaggtgcaaa acgcacccgg
       61 acgccgctct gcgaaccgcc tgaatgtccg gacatgaacc gttctaaccc ttttggaaaa
      121 attacccttg ggaacgctgt aagttacagt gtaaaggaac cttacgggag cgtccaacgt
      181 ctccccgaac cactgctatg gtttggcctg tccctagttg cgatctcgcc ctgctgtcac
      241 ttacgcaacc agtacgccgg tacgcttgcg cacctaattt tccctgcacc ataatcgcaa
      301 gtactttgtt ttgtgcagag acggtcggtt tggtcacccc acgagtcctc gcgtgagcgg
      361 cgatctaccg actcctggca cacgctgcac cgcaggaagg gcgcagggcg tttgctcgcc
      421 ggacggagaa gcgatagctc gttgatcaat ctcagcctag tctcgttggc gctgctcatt
      481 gccacttcct tggtggctgg cttcttcgca tacgtttaca acctcaagcg gcaaacgtac
      541 ttgctttgct ggaccgccgg ctggtgtctg ctggccctgc attatctcgg cacgggcctg
      601 agtggtagcc tcggcccgtc cgctggccag cgcgccgtcg atcaggctct tttttgcggc
      661 gctggcattc tcttttttgc gggcgccaag ctctacgccc agcggcgggc cacgcttcca
      721 gtgacgatcg gcgccggcgc ggtcgtgctg ctgtggagca tcgccaacgc ttttggaatc
      781 attcccctgc cagttacttt cgcggcggcc ttaatctacg gcgccgtggg ctttgaattt
      841 tgggtagaaa gccagcgcca ggaaacgctc gcggactggc tgctcgccgt cgtgtttttt
      901 gcgtggggcg cactcggcct gatgttcctg ttctttcagc gctcgctcag ctcatggaac
      961 atcccttttg aaccaatctc cgcattgccc gccacgttcg cggcaatgct gatggtcatg
     1021 gcgctttacg aagaggaaaa acgccgtatc gagcggaaca tgctggcgct ttccaatctg
     1081 aatctcgccg cttcgggctt tgccggcggc gaaattcatc gcatgctctc gcaagcgttg
     1141 gaccgcgttc tgggcgtggt gcgcctacct gtgggggcgc tttttctgca tcatggcgat
     1201 ccacaaggac cgacttccgt tgtggcggtc ggcctgaatg aaaaattctg ccggctggcc
     1261 caggaagaag ggctggacga ttacctcgtc aatttggtct ccagccttgg cggcttgatg
     1321 ggcttccgcg atttgcgcga cgacgacctg atggctctcg agaaaaagga ccaacgcatc
     1381 cgaaggttcc gcgagcttgc cctggagcag cgtctgcgca gcatggtggc cattagcctt
     1441 caggccaaag agcaggcttt cggcgtgctt ctcctcggct cacccgatac tcgccggttc
     1501 gcccctgccg agttacgcct gctgctggcc cttggccatc aaatcggcat ggccgttgaa
     1561 aatagtcttt tgatccagca gacttcccgc cgttccgagg aactgcacgt cttgaacgaa
     1621 atcggccgcg cactgagttc caccctcaac aaggaagatc ttctgcgcaa ggtctgggaa
     1681 gagttgcgcc gcttgttcga cgtggaaaat ttctacatcg cctccctgga tccgattggc
     1741 gacgaaatgc agttcgaact ggaaattatc gacggcatac gtatgccgcg ccgcagcaga
     1801 ccggccggca acttcttgac ggaatacgtc atccgtaccc gccagcctgt tctgattcgc
     1861 gagaatttcc tcgacgaggt tcgcaaattg aacgtcgatc cgatccgcgt gcagggatgc
     1921 ttttgcggcg ttccattggt tgcctacgat cgcgctatcg gagccatggc tgtcttctct
     1981 gaccaggaca aaaccttcga cgaagggcat ctcgaacttc tacgtgtgct ggccagcgaa
     2041 gccagtatcg ccatcgagaa tgctcgcctc tttcaggaag agcgcaccaa ggcccgccac
     2101 ctgacgctgc tcaacacgat ttcccgccat gccatcggta ctcttaatcc cgacgagatg
     2161 ctggcaaaaa tgaccgagga gctggagact ggcctcacct acgatcacat cggcatcgcc
     2221 gtcctcgact attcgacgcg cgagctggtg attcaagccg aaggcggcaa acgacgcggc
     2281 gccttagggc agcgtgtgcc gctcggatcc ggactcatcg gcaacgtcgc tcgcgcagga
     2341 aatctcgtca cgttccgctc cagtcttgcc gccgacgccg ccatgcgacc catgctcgct
     2401 gattccgtcg cggccgtcgg cttacccatc ttctttgcgg aacaacttca cggcgtactt
     2461 tacgtcgagt cttccgagaa cgttgatttc accgaagaag agatcctgtt gctcggaacc
     2521 ttggccgatt tgatcgccgg cgctctccac aacgctctca cgatccagaa agc
//
LOCUS       DASTPM010000749         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_257508, whole genome shotgun sequence.
ACCESSION   DASTPM010000749 DASTPM010000000
VERSION     DASTPM010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257508"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VFR42_13235"
     CDS             <1..1025
                     /locus_tag="VFR42_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HEU0370174.1"
                     /translation="ICNCFLSRFEQFHAHFFTILESLPRCMSRVAHSPRLALDVKLTI
                     MANKFTVGLVQMRCTTDAEENLSRAIDKIREASARGAQIISLHELFRSEYFCRKEDAD
                     LFNLAEPVPGPATEALAKVAKEKKVALVVSLFERRAAGIYHNTCAVLDADGSFLGKYR
                     KMHIPDDPLYYEKFYFTPGDLGFPNFETKYGKIGVQICWDQWYPEGSRVTALSGAQVI
                     FYPTSIGWHPHEKAEFGAAQLDAWKTIQRAHAIANGVYIAVVNRVGFEGKPANGDPGL
                     EFWGNSFVADPFGQIAVQASDDKEEILVVECDPAKSEDTRRNWPFLRDRRIDAYGPIL
                     NRWLGE"
     gene            1031..2104
                     /locus_tag="VFR42_13240"
     CDS             1031..2104
                     /locus_tag="VFR42_13240"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEU0370175.1"
                     /translation="MPPAQSSLTPFERGFWMPAEWEHHEATWLGWPHEKTDWPGKFPA
                     IPWAYAEIVRHLARVERVYLLVQNRAAESQARDALKKSGVNLGAIEFFRVPTDRGWMR
                     DSGPICVRSHSGEVAYTHWLFNGWAKYSNHKKDALAVSNVNKKLRRTTWQPMHNGRRV
                     VLEGGSIDVNGRGSLLTTEECLLSKRQQRNHGFTREDYAHVFRSYLGVTQVLWLKNGI
                     AGDDTHGHVDDLARFVSPTTIVTVVEQDPHDANYVPLQENLALLKSMKDLNSRPFRIE
                     TLPMPAPIYFDNQRLPASYANFYIANKLVIVPTFNDPNDRVALNMLAGLFPGREVVGI
                     ACRDLVLGLGTLHCLTQQQPAGA"
     gene            2218..>2620
                     /locus_tag="VFR42_13245"
     CDS             2218..>2620
                     /locus_tag="VFR42_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0370176.1"
                     /translation="MQLNLNLLKATVVGALGGLLFGFDTAVIAGTTHSLTVGYGLSPR
                     QLGLTVSMALIGTVIGAMSAGIPGQKYGARETLRVLAVFYVLSALGCAFAWNWDALLV
                     ARFLGGLGIGGSSVLGPVYIAELAPARIRGRL"
BASE COUNT          532 a          840 c          695 g          553 t
ORIGIN      
        1 aaatttgcaa ctgcttttta tcacgatttg aacagtttca cgcgcatttt ttcaccatcc
       61 tcgagtcatt gccgcggtgc atgagccggg tggcgcactc gccgcggctt gctctcgatg
      121 ttaaactaac aattatggcg aacaagttta cggtcggcct cgtccagatg cgctgcacca
      181 ccgacgccga ggaaaatctt tcccgcgcga tcgacaaaat ccgcgaagcg tcggcgcgcg
      241 gcgcacaaat catctcatta catgaactgt ttcgtagcga atatttctgc cgcaaggaag
      301 acgccgacct tttcaatctc gccgaacccg tgcctggccc cgcgaccgag gccctcgcca
      361 aggtggcgaa agagaagaag gtggcgttgg tcgtttctct gttcgaacgc cgggccgcag
      421 gcatttatca caacacttgc gccgttctcg acgccgacgg cagcttcctc ggcaaatatc
      481 gcaagatgca cattcccgac gacccgctct actacgaaaa attctatttc accccggggg
      541 atcttggttt cccaaatttc gaaacgaagt acggaaaaat tggtgtgcag atttgttggg
      601 accagtggta tccggagggt tcgcgcgtca cagccctcag cggcgcccag gttattttct
      661 acccgacaag tatcggctgg cacccgcacg aaaaagcgga attcggcgcc gcgcagctcg
      721 acgcctggaa aacgatccag cgcgcccacg cgattgccaa cggtgtctac attgcggtgg
      781 tcaatcgcgt tgggttcgag ggaaaacccg ccaatggcga tcccggcttg gaattttggg
      841 gaaattcgtt cgtcgctgac cctttcggcc agatcgcagt ccaggcttcc gacgacaagg
      901 aagaaatcct cgttgtcgaa tgcgatccgg cgaagagcga agacacccgc cgcaactggc
      961 cctttctccg ggatcgtcgc attgatgcct acggtccgat cctcaaccgc tggttgggcg
     1021 aataatccgc atgcctccag cccagagcag ccttacgcca ttcgaacgtg gtttctggat
     1081 gcccgctgaa tgggagcacc acgaggcgac ttggctcggc tggccgcatg aaaaaaccga
     1141 ctggcccgga aaatttcccg ccatcccctg ggcctacgcg gaaatcgttc gtcacctcgc
     1201 gcgcgtcgag cgtgtttatc tgctcgtaca aaatcgcgcc gccgagtctc aagcgcgtga
     1261 cgcgctgaag aagtccggcg tgaacctggg tgccattgaa ttctttcgcg tccccaccga
     1321 ccgcggctgg atgcgcgact ccggtcccat ctgcgtccgc agccattccg gcgaagtcgc
     1381 gtacacccat tggctcttca acggctgggc caagtacagc aatcacaaga aagatgccct
     1441 cgccgtatcc aacgtcaaca aaaagctcag gcgcacaact tggcagccca tgcacaacgg
     1501 ccgccgcgtg gtcctcgaag gcggcagcat tgacgtcaac ggtcgcggtt ccctactcac
     1561 taccgaagaa tgcctgctca gcaagcgtca gcagcgcaat cacggattca cccgcgaaga
     1621 ttacgcccat gtttttcgca gctatctcgg cgttacgcag gttctctggc tgaaaaatgg
     1681 catcgccggc gatgacaccc acggccacgt tgacgatctg gcccgctttg tcagtcccac
     1741 caccatcgtt accgtagtgg agcaagatcc tcacgacgcc aattacgttc ctctccaaga
     1801 aaatctcgcc ctcctcaaat ccatgaaaga tctcaacagc cgccccttcc gcatcgagac
     1861 tcttcccatg cctgcgccga tctatttcga caaccagcgc ctccccgcca gctacgccaa
     1921 tttctacatc gccaacaaac tcgtcatcgt cccgaccttc aacgacccaa acgatcgagt
     1981 agccctgaac atgctcgccg gcctctttcc tggacgcgaa gtcgttggca ttgcctgtcg
     2041 cgacctcgtc ctcggcctcg gcactctcca ctgcctgacc caacagcaac ccgccggcgc
     2101 ttagagaatc ctctgcacga ctcggtctca gcgcctcttc aaaaggcttg acgaccggga
     2161 cgatgcaggg gacgatatgc ggccaatgac cggcagcggt cttcagagca caatttaatg
     2221 caacttaatc tgaacctttt aaaggcgacc gtggttggcg cgctcggcgg actcctgttc
     2281 ggcttcgata ctgctgtcat cgctggtacg actcattcgc tcacggtcgg ttacggcctt
     2341 tcgcctcggc agcttggtct tacggtctcc atggcgctga tcggcaccgt gatcggcgcg
     2401 atgtccgccg gcatccctgg gcagaagtat ggcgcgaggg aaactcttcg cgtgctcgcg
     2461 gtcttctacg tgctgtccgc gctaggctgc gcgtttgcct ggaattggga tgcgctgctc
     2521 gttgcgcgat ttctgggcgg tctcggcatc ggcggctctt cggtgctcgg gccggtgtat
     2581 atcgcggagc ttgctccagc cagaattcgc ggccgcctgg
//
LOCUS       DASTPM010000750         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1295647, whole genome shotgun sequence.
ACCESSION   DASTPM010000750 DASTPM010000000
VERSION     DASTPM010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295647"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VFR42_13250"
     CDS             complement(<1..973)
                     /locus_tag="VFR42_13250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0370177.1"
                     /translation="MPSAKKFLVLCTAFGMALGAGPSALPHRQEPSQQQGPVIRTQVN
                     LVNLFVTVRDKSKRIVTDLKQDDFKIQEDNQPQQIAFFSKEVTLPITLALLLDTSGSE
                     QYMLPAIQDAGSRFIDRVLRKGDETLVMSFDSDVDLLSDFTDDRGQIDRAIHKARINV
                     PSGGSIGNNPGPIGSRQITGTALYDAIYLACNEKLNTEAGRKAIVIVTDAQDEGSKVR
                     LEEAVEAAQRTDTVVHILLVADSHFGGGNGGVARKITDETGGRTIVVRSEKNLEQAFD
                     QISQELRSQYTIGYYSSNTTRDGGYRKIKVDTARKDLDVLTRRGYYAS"
     gene            1121..2254
                     /locus_tag="VFR42_13255"
     CDS             1121..2254
                     /locus_tag="VFR42_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0370178.1"
                     /translation="MKSLRAIAASAIFGVFGFAMYVAGQTAGQSAPAPATGSLQNVPA
                     PAGQRTQTAPPPSDARAKISVNSNLVVLPVTVKDRSGNLVPDLRRDEFRVFEDNVEQR
                     IDVFTAEAFPLSMVLLLDNDLKEKDAQQVRKSLDAVVGGLSVEDEAFICRFDQFFHPG
                     KGFTADHDVLLTELQRTTLDTQPSVAPPSGGIANGPSINGHSAIGDAPNIAPATIAIG
                     NQPTKALDDAVFGAAQLLHDRNRNRRKIILLISDGANGGKKTNKMTYDSVVKTLQHDN
                     IEVYSVAVASAFLERKFSRLVEYAHDSGGEVFFAAKRETLEELYSRISEEARNQYTIA
                     YVPRGTDRSAEMHSIEVRVKREGLNIKTRDRYYAGEIPGATPQ"
BASE COUNT          462 a          594 c          803 g          461 t
ORIGIN      
        1 tcgaggcgta gtagccccgc cgggtgagga cgtccagatc cttgcgcgcc gtgtctacct
       61 taatttttcg gtagcctccg tcgcgcgtgg tgttgctcga atagtagcca atggtgtact
      121 ggctgcgcag ttcctggctg atctgatcga aggcttgctc gagattcttc tcgctgcgaa
      181 cgacgatggt gcggccgccg gtttcgtcgg taattttgcg ggccacgccg ccattgccgc
      241 cgccaaagtg ggaatcggcg accaataaaa tgtggacgac cgtgtcggta cgctgggcgg
      301 cttcgaccgc ttcttccagg cgaaccttgc tgccttcgtc ctgggcatcg gtgacgatga
      361 cgatggcttt gcggccggcc tcggtattga gtttttcgtt gcaggctaga taaatggcgt
      421 cgtagagcgc ggtgccggtg atctggcggg agccgatggg gccggggttg ttgccgatgc
      481 tgccgccgga aggaacgttg atgcgggcct tgtgaatggc gcggtcgatc tggccgcggt
      541 cgtcagtgaa atcggagagc aagtcgacat cggaatcgaa agacatgacc agggtttcgt
      601 cgcccttgcg cagcacgcga tcgatgaaac ggctgccggc gtcctggatg gcggggagca
      661 tgtattgttc gctgccggat gtatcgagaa gcaacgccag ggtgatggga agcgtcactt
      721 ctttggagaa gaaggcaatc tgttgcggct gattgtcttc ctggattttg aagtcgtcct
      781 gcttgaggtc ggtgacgatg cgtttgctct tgtcgcgcac ggtgacgaac aggttgacca
      841 ggtttacctg cgtacggatg accgggcctt gctgctgcga cggttcctgc cggtgcggaa
      901 gcgcggacgg gccagcgccc agggccatcc cgaaggcggt gcagagcacc agaaacttct
      961 ttgcagaagg catgtatact ttccttgtac aggatacccg gtcagaagtg cccgacgcca
     1021 ggatgcttac gtcccgtaag atgcgggaag gagcggcgcg ggatgcgtgg ctcggccccc
     1081 cggagctgcg ggaaaccacg gaaagtttgg attgagaacc atgaagagtc tacgagcaat
     1141 cgccgcgtcg gcgatatttg gagtttttgg atttgcgatg tacgttgccg ggcaaacggc
     1201 ggggcaatcg gcgccggcac cagcgacggg ttccctacaa aacgtgccgg cgccagccgg
     1261 gcagcggacg cagacggcgc ctccgccgag cgatgcgcgg gcgaaaatca gcgtgaattc
     1321 gaatctggtg gtgctgccag tgacggtgaa ggaccgctcc ggcaacctag tgccggattt
     1381 gcggcgcgac gagtttcgcg tgtttgaaga caatgtggag cagagaatcg atgtgtttac
     1441 ggcggaagcg tttccgctgt cgatggtgct gctgctggac aacgatttga aagagaagga
     1501 cgcgcagcag gtgcgcaaga gcctggacgc agtggtgggg ggcctgagcg tggaagatga
     1561 ggcgttcatc tgccgcttcg accagttttt tcatccgggg aaaggattta cagcggatca
     1621 cgatgtgctg ttgacggaac tgcagcgcac cacgctggat acccagccgt cggtggcgcc
     1681 gccgagcggc ggcattgcca acggcccatc gatcaacgga cattcggcga ttggcgatgc
     1741 gccgaacatc gcgccggcaa cgatcgcgat tggcaatcag ccgaccaagg cgctggatga
     1801 cgcggtgttt ggcgcggcgc aactgttgca cgaccggaat cgcaaccggc gcaagattat
     1861 cctgctgata tcggacgggg ccaacggggg gaaaaagacg aataagatga cgtacgacag
     1921 cgtggtaaag acgctgcagc acgacaacat cgaggtttat agcgtggcgg tggccagcgc
     1981 gtttctggaa cgcaagtttt cgcggctggt ggagtacgcg cacgattccg gcggcgaagt
     2041 gttttttgcg gccaagcggg agacgctgga ggaactttac tcgcggatca gcgaagaggc
     2101 gcgaaatcaa tatacgattg cgtatgtgcc gcggggaacg gaccgcagcg cggagatgca
     2161 cagcatcgaa gtgcgggtga agcgcgaagg gctgaacatc aagacgcgcg accgctatta
     2221 cgcgggggag attccgggag ctacgccgca gtagggtgat tttgttttgc ggaaagcgac
     2281 gaatcatctt gacagcgacg ggccgcgccc gtatatcaag
//
LOCUS       DASTPM010000751         2428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1180867, whole genome shotgun sequence.
ACCESSION   DASTPM010000751 DASTPM010000000
VERSION     DASTPM010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180867"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFR42_13260"
     CDS             <1..1038
                     /locus_tag="VFR42_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370179.1"
                     /translation="RGRPFEITEGVSCENCHGPAQAWLGQHTEGESPEKHARSLALGM
                     ADTRDVIHRTEKCLQCHLGTAQKFVDHEMIAAGHPDLFFELDSFSAVMPRHWKQPRES
                     AHGKPAPGPDGDANWTSVRDWSAGQAVQLRAAMERLTWRAKNERSDKRDAWPEYSELS
                     CFACHHSLAPAKDSWRQEHGYTGRRPGDPAWNGSRYVVFRVLARQVDPTAAKELDQRL
                     AAVADEMSKLNPDRAAVASAATSAVPVAQRFAERLATMSYDSATALRAMQGITQDADA
                     IALADERAAEQAAMALDSLYIAYRKQANPTNAAEVREAINGLFQQVENPSAYNADRFA
                     SALKRIGDLIR"
     gene            1132..1710
                     /locus_tag="VFR42_13265"
     CDS             1132..1710
                     /locus_tag="VFR42_13265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0370180.1"
                     /translation="MNRFENWFCASSIWRFVTERKLLPWIVSGYPLGDHVLEVGAGPG
                     ATTEELLRRAGRVTSLEYDPRFVTRLAEKHKDKGARVTVVRGDAAALPFPDHSFSSVV
                     AVLVLHHLRSREQQDCAFQEFHRVLRPGGIFFAFEIRDGWLHRVGHYKSTFVPVAPAS
                     TFGRLTLAGFSRIAVDIERSAFRVCALRAREA"
     gene            1851..>2428
                     /locus_tag="VFR42_13270"
     CDS             1851..>2428
                     /locus_tag="VFR42_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0370181.1"
                     /translation="MSSSSVVTARRGAEKFPPGPPPNLLRALFGAMQQNPLDYFTELA
                     QKYGDVSGMRIGNFRTLFINHPDLIEDVLVNKARLYHKGRILQSNKYLFGEGLLTSEG
                     DFWLRQRRLSQPAFHRERIAAYAATMADYTEQMLATWRGGEERDVHEEMMNLALRIVG
                     KTLFDADVTRDAKEVGETLDILLEIAANFGRTV"
BASE COUNT          482 a          702 c          763 g          481 t
ORIGIN      
        1 cgcggacgcc catttgaaat aacggaagga gtgagctgcg aaaattgcca cggcccggca
       61 caagcctggc tcggccagca caccgaaggc gaatcaccgg aaaagcacgc acgttctctc
      121 gcgctcggta tggccgacac ccgcgacgtg attcatcgta ctgaaaaatg cctgcaatgc
      181 cacctcggca cggcgcaaaa atttgttgat cacgaaatga tcgcggctgg ccatcccgat
      241 cttttctttg aactcgattc tttctccgcc gtgatgccgc ggcactggaa acagccgcgc
      301 gagtctgctc acggcaagcc ggcccccggg cccgacggtg acgccaactg gacttccgtg
      361 cgcgattgga gcgccggcca agccgtgcag ctgcgcgctg cgatggaacg cctcacctgg
      421 cgcgccaaaa acgaacgctc cgacaaacgc gatgcgtggc cggaatattc cgagctttct
      481 tgtttcgcat gccaccactc cctggccccg gcaaaggata gttggcggca ggagcatggc
      541 tacactggcc ggcggcctgg cgatcccgcg tggaacggat cgcgctacgt ggtgttccgc
      601 gtcttggcgc ggcaggttga tccgactgct gcgaaggaac tggatcagcg cctcgccgcc
      661 gttgcggatg agatgagcaa gctcaatccc gatcgcgctg cagttgcgtc cgctgcaact
      721 tcagctgtgc cggtggcgca gcgatttgcg gaacgcctcg ctacgatgtc atacgactcc
      781 gcaactgcgc tgcgtgccat gcagggcatc acgcaggacg cggatgcgat cgcacttgcc
      841 gacgagcgcg ccgcggagca agcggcgatg gcgctcgatt cgctgtacat cgcgtaccgc
      901 aaacaagcca acccgacaaa tgcggcagaa gtgcgcgaag ccatcaacgg attattccag
      961 caggtggaga atccgtcggc ttacaacgct gaccgctttg cctcggccct caagcgaatc
     1021 ggcgacctga tccgttgaat taagaatcta ttgcatcaaa attttcggct ttccttctcg
     1081 ggcccgacgg gtctaaagta aggagtaacc tcgccaggct ggctcccttt tatgaatcgc
     1141 tttgaaaact ggttttgcgc atcttccata tggcgttttg tcaccgaacg caagctgctg
     1201 ccgtggatcg tgtcggggta cccgctgggc gaccacgtgc tggaagtggg cgcaggaccc
     1261 ggcgctacga ccgaggaatt gctcaggcgg gctgggcgcg tgaccagcct ggaatacgat
     1321 ccgcgctttg tgacgcggct ggcggaaaag cacaaggaca agggcgcgcg ggtaaccgta
     1381 gtgcgtggcg acgcggcggc tttgccgttt ccggaccatt cgttttcttc ggttgtggcg
     1441 gtgctggtgc tgcatcattt gcgttcgcgg gagcagcagg actgcgcatt ccaggaattt
     1501 caccgcgtgt tgcggccagg cggaatattt tttgcgtttg agattcgcga cggctggctg
     1561 caccgcgtgg gacattacaa gagcacgttt gtgccggtgg caccggcgtc gacgtttggc
     1621 cgtttgacgc tggcgggatt ttcgcggatt gccgtcgaca ttgagcgatc ggcgtttcgg
     1681 gtgtgcgcgt tgcgggcgcg agaagcgtaa ggctggccgc ttttctgatt tggcaacgaa
     1741 gggaaaagca cgggtctaag gacccgccac tacaccagcg gagacggccg ctgcaaagac
     1801 gttatattgg ccgtgttgac cgcacccgca ctgttctcta gcatttctgg atgagcagtt
     1861 cttccgtggt gacggcgcgg cgaggcgccg aaaagtttcc tccggggccg ccgcccaatt
     1921 tgctgcgcgc gctgttcggc gcgatgcagc agaatccgct ggactacttc accgaactgg
     1981 cgcaaaagta cggcgacgtt tccggcatgc gcatcggaaa tttccgcacg ctgttcatca
     2041 atcatcccga cctgatcgag gatgtgctgg tgaacaaagc gcggctctat cacaagggcc
     2101 gcatcctgca atcgaacaaa tatttgttcg gcgaagggct gctgaccagc gaaggcgatt
     2161 tctggctgcg gcagagaagg ctttcgcagc cggcgtttca ccgcgaacgc attgcagcat
     2221 acgccgcgac gatggcggat tacacagaac agatgctggc tacatggcgg ggcggcgaag
     2281 agcgcgacgt ccacgaagag atgatgaacc tggcgctgcg cattgtcggc aaaactttgt
     2341 ttgatgcgga cgtgacgcgc gacgcgaaag aagtgggcga gacgctggac attctgctgg
     2401 aaattgccgc gaatttcggg cgcacagt
//
LOCUS       DASTPM010000752         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_402007, whole genome shotgun sequence.
ACCESSION   DASTPM010000752 DASTPM010000000
VERSION     DASTPM010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402007"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2185
                     /locus_tag="VFR42_13275"
     CDS             <1..2185
                     /locus_tag="VFR42_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370182.1"
                     /translation="PALDQIVALLPTSDSNSLPYSVRDKNDGDNFIVKIDHHIRENVT
                     LTARYAFGQSDQIFPLGSLGGFGSGSRLQQFTQESPTRVQVLSTSLLTTLGTARLNEL
                     RFGYSRFRTNFNSTDEKTIDPAAFGLDMGTGKTGLPEIDFNGDIENLGATAFSVPRGR
                     VSETFQVLDNFTWIRGRHTLKFGAEYHRYDVQSFNDNLERGLLDVNTCVSLSDTDCPT
                     LSDDPIVNELANFYIGNIFAEANAGRTQRFTFNNNLSFFTQDEFRLRPNLTLTAGLRW
                     EYYGPLSEKNNLLSNFDARGNLVQVGSPTLPRVYNRDLNNFAPRIGLAWNPRRSTVVR
                     AAYGLYYDYIPQNVLIANFTNSAGVATNPVSALSGTPNFVGSLDFNQDAFNGTVSGPV
                     FTPTTNPQSIFVTDHNLRTPYVHSWNLNLQQQLGGSFAVETGYVGSKGTKLTRLYDQN
                     QDFTNPLYSVIDVFSTGANSTYNAWQTTVRMRNWHRFSGFSTYTWSKSLDGASDGIDF
                     NFATAAFPQNSDNLAAEKGPSTFDTRHRWTTAINYQVPTLSHLPKLLGEGWQWNSIIT
                     VQSGRPIGIVTSDDTSGTFNFHQRPNVVPGVNPILSHWSPDTGYLNSAAFAQPDFGTF
                     GDLGRNQIYGPGFWNIDFSLSKNFQLRDRLMLQFRSEFFNIFNHPNFALPSNTITPGI
                     NADGSVNNFAGPAGVITQTPDVAQGNPGLGGGGPRVLQFGLRLQF"
     gene            complement(2274..3014)
                     /locus_tag="VFR42_13280"
     CDS             complement(2274..3014)
                     /locus_tag="VFR42_13280"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0370183.1"
                     /translation="MSNLSNKVAIVTGGSRGIGAAIAKRLAADGANVAITYTKGADAA
                     ASVVKEIERAGAKAIAIQADAADAKAVEAAVEKTVSTFGQLDVLVNNAGTAIPKKFEE
                     TPLEELDRVIDINLRGTFVATQTALKHMKNNGRIIMIGSCVGERILTPGLVPYSATKG
                     AVKMFTQGLAREVGDRGITVNNVQPGPIDTDLNPAAGDWAAPQKANTALNRYGTVDEV
                     ASLVAFVASPAAAYITGANLTVDGGTNA"
     gene            3325..4533
                     /locus_tag="VFR42_13285"
     CDS             3325..4533
                     /locus_tag="VFR42_13285"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU0370184.1"
                     /translation="MPEAVIVSAVRTPVGRAYKGTLRATRPDDLAALVIKEAIARVPS
                     LSACEIDDVILGCAMPEGEQGMNVARIAALRAGLPVETSAMTINRFCSSGLQAIALAA
                     DRVRGGGAEVIVAGGTESMSMVPMGGNKISPNPWLVDHYPDAYINMGLGTENIARKFG
                     ISRDAADEFALNSHKKAVAAIAAAKFKDELLPVDVSITSLPPNGNGASGKGKTQKPTT
                     QTVRFDTDELPRADTSLEVLAKLKPAFHVKGSVTAGNSSPMSDGAAAVVVMSDARAKS
                     LGVKPLARFVAYATAGCLPEEFGIGPVNAIPKALKIAGLKLDDIAVIELNEAFAAQSL
                     AVIQQAGLPAERVNPNGGAIALGHPLGCTGAKLTASIVRELERRDARYGMVTMCIGGG
                     MGAAGIIERM"
     gene            complement(4467..>4811)
                     /locus_tag="VFR42_13290"
     CDS             complement(4467..>4811)
                     /locus_tag="VFR42_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370185.1"
                     /translation="LSLLISSGNSVSEGFSGDANFKLITNGEYTRLVFSSHAQFVSLS
                     DRVFEPVLTIAMQRKIRDDLANLKILAQAQSEKSANPRASLWCIGNLGNYILSMIPAA
                     PMPPPMHMVTMP"
BASE COUNT         1019 a         1565 c         1183 g         1044 t
ORIGIN      
        1 cccggcgctc gaccagattg tcgcgctcct tccgaccagc gattccaatt cgcttcctta
       61 ctccgtccgc gacaaaaacg acggcgacaa cttcatcgtc aagatcgatc atcacatccg
      121 cgaaaacgta acgctcaccg cccgctacgc cttcggccag tcggatcaga tttttccgct
      181 tggctcgctc ggcggattcg gcagcggctc ccgcctccaa caatttacgc aggaatcgcc
      241 cacgcgagtg caggtcctct ccaccagttt gctcacgact ctcggtacgg cgagactcaa
      301 cgagctgcgt ttcggctatt cgcgctttcg caccaatttc aattccaccg acgaaaagac
      361 cattgacccg gctgcatttg gcctggatat gggcacggga aaaaccggct tgcccgaaat
      421 cgatttcaac ggcgacatcg aaaaccttgg tgcaaccgcc tttagcgttc cgcgcgggcg
      481 cgtcagcgag accttccagg tgctcgacaa tttcacctgg attcgcggac gccacacact
      541 gaaattcggt gccgagtatc accgctacga cgtgcaaagt tttaatgaca atctcgaacg
      601 cggtcttctc gacgtgaaca cttgcgtttc cctgagtgac accgactgcc cgaccctctc
      661 ggatgaccct atcgtgaatg agctggcgaa tttttatatc ggaaacatct tcgctgaggc
      721 caatgctggc cgcactcagc gcttcacgtt caataacaac ttaagcttct ttacccagga
      781 cgagttccgt ctgcgcccca atctcaccct caccgcagga cttcgctggg aatactatgg
      841 tccgctgagc gaaaagaaca atttgctctc aaattttgac gccaggggca acctcgtgca
      901 ggtcggttcg cccaccttgc ctcgcgtgta caaccgcgac ttgaacaact tcgcgccgcg
      961 catcggcttg gcctggaacc caagacgtag caccgtggtt cgcgccgcgt acggtttata
     1021 ctacgactac atcccgcaga acgtcctgat cgccaacttc accaattccg ctggcgtcgc
     1081 aacaaatccc gtgagcgctc tcagcggcac gccgaatttc gtcggctctc tcgatttcaa
     1141 tcaggacgcc tttaacggca cagtttctgg tcccgtcttc actccgacca ccaatccaca
     1201 gtccattttc gttaccgacc acaacttgcg gactccctac gtgcacagct ggaatctgaa
     1261 cctgcaacag caactcggcg gcagttttgc cgtcgaaacc ggttatgtcg gcagcaaagg
     1321 taccaagctg actcgcctct acgaccaaaa tcaggacttc accaatccgc tttactccgt
     1381 cattgatgtc ttctcgaccg gagcaaactc gacgtacaac gcctggcaaa ccaccgtccg
     1441 catgcggaac tggcatcgct tcagcgggtt ctcgacctac acctggtcaa aatccctcga
     1501 cggtgcttcc gacgggattg acttcaactt cgctacggcc gcattcccac aaaactcaga
     1561 caatctcgcg gcggagaaag ggccatccac cttcgacacg cgccatcgct ggaccaccgc
     1621 aataaactat caggtgccaa cgctaagcca tctgcccaaa cttctcggcg aaggttggca
     1681 gtggaactcc atcatcaccg tgcagtccgg ccgacccatt ggcatcgtca cctccgacga
     1741 caccagcggc accttcaatt tccaccagcg ccccaatgtc gttcccggcg taaatccaat
     1801 cctttcgcat tggtcgccag acaccggata tctgaattcg gccgcattcg ctcagccgga
     1861 cttcggcaca ttcggcgacc tgggacgcaa tcagatttat ggccctggct tctggaacat
     1921 cgatttctcg ctcagcaaga atttccagct cagagatcgg ctgatgctgc agttccgctc
     1981 agagttcttc aacattttca atcatccaaa ctttgctctg ccgtccaaca ccatcacccc
     2041 gggcatcaat gccgacggca gcgtgaacaa ttttgcggga ccggcggggg ttattacgca
     2101 aacccctgac gttgcccaag gcaatcccgg cctcggcgga ggcggcccgc gcgttctcca
     2161 attcggcctt cgcctccagt tctagcagca gtttaagcga tgcgggaccc gccttctgat
     2221 tgggacgaag cttcttccgt ctttaagaag tgcaaacctt tttcgttggc cggttaggcg
     2281 ttcgttccgc cgtccactgt caggttcgcg ccagtaatgt aggctgccgc tgggctggcc
     2341 acgaacgcaa cgagcgaggc tacttcgtcc actgtcccat accggttcag cgccgtattc
     2401 gccttctgcg gcgcggccca atcgcccgcc gccggattca ggtcggtatc aatagggccc
     2461 ggctgcacat tgttcactgt aataccccgg tcgccgacct ctcgcgccag tccctgcgtg
     2521 aacatcttca ctgcgccttt agtcgccgag tacggcacca ggcccggagt caagatgcgc
     2581 tcgcccacac aggaaccaat catgatgatt cggccgttgt tcttcatgtg ctttagcgcc
     2641 gtttgcgtcg ccacaaacgt gccgcgcaga ttgatgtcaa tcacgcgatc tagttcctca
     2701 agcggtgtct cctcaaactt cttgggaatc gccgtgcctg cattgtttac cagcacgtcc
     2761 agctgtccaa atgtcgaaac cgttttttcc accgccgctt ccaccgcctt ggcatccgct
     2821 gcgtccgcct gtatcgcgat cgcctttgcg ccagctcttt caatttcctt gaccactgaa
     2881 gccgctgcat cagccccttt cgtgtaggta atggcgacgt ttgctccatc cgctgccagg
     2941 cgcttggcta tggccgcgcc aatcccgcgc gatccacccg tcactatcgc taccttgttt
     3001 gatagattcg acataaatcc tccaagtccg aatattcaag tcagcccggc cgatccgact
     3061 gacgtgatcg tggatcgcgt cgagtgcaga acagatgcaa gaacccgcac tttcgttacg
     3121 acagactttt gctggaactc tttcgtatcg ttcatggccg acatggcgga tcccttgcgt
     3181 agtcccgtga aacttttcag acaccagttc cgcttcgtaa caacctcgtg agaactcagt
     3241 tagcggttcc ctcttcggcc ttcggcctgc aggatttgct ttttccctgc aaccagccaa
     3301 aaatgaattt tgggagaaat gcccatgcca gaagccgtca tcgtctccgc cgttcgcaca
     3361 cccgttggtc gcgcctacaa aggcacgctc cgcgccaccc gtcccgacga tctcgccgct
     3421 ctcgtcatca aggaagcgat cgctcgcgtt cccagtctct ccgcttgcga gattgacgac
     3481 gtaattcttg gctgcgccat gcccgaaggc gaacaaggca tgaacgtcgc gcgcatcgcc
     3541 gccttacgcg ccggcctgcc cgtagaaact tccgccatga ccatcaaccg tttctgctct
     3601 tcgggattgc aagccatcgc tctggccgcc gatcgcgtgc gcggcggcgg cgccgaagtc
     3661 atcgttgccg gcggaactga atccatgtcc atggtcccaa tgggcggcaa caaaatttct
     3721 cccaatccct ggctggtcga tcattatccc gatgcctaca tcaacatggg actcggcacg
     3781 gaaaatattg cccgcaaatt cggaatctcg cgcgacgcgg ccgatgaatt tgccctgaat
     3841 tcgcacaaga aagcagtcgc cgcgatcgcc gcggccaaat tcaaagatga acttcttccc
     3901 gtcgacgtca gcatcacttc tctgccgccc aatggcaacg gcgcttccgg caaaggcaaa
     3961 acgcagaaac cgacgacgca aaccgttaga ttcgacaccg acgaattacc gcgcgcggac
     4021 acttctctgg aagtcctcgc caaactcaag cccgcgtttc acgtgaaagg ttccgtgacc
     4081 gcaggtaaca gttcgccgat gagtgatggc gcggccgcgg tcgtggtgat gagcgacgcg
     4141 cgcgcaaagt cgctcggcgt gaaaccgctg gcgcgtttcg tcgcatatgc aactgcgggc
     4201 tgcttgccgg aagaattcgg catcggcccg gtcaatgcaa ttcccaaagc tttgaaaatc
     4261 gccggactaa agctcgacga catcgcggtg atcgaattga acgaagcatt cgcggcacag
     4321 tcgctggccg taattcagca agccggactg cccgcggaaa gagtaaatcc caacggcggc
     4381 gccatcgctc tcggccatcc gctcggctgc acgggcgcaa aactcactgc ctccatcgtg
     4441 cgcgaactcg aacgccgcga tgcccgctac ggcatggtca ccatgtgcat cggtggaggc
     4501 atgggcgctg cgggaatcat cgagaggatg taattcccca gattaccaat acaccagagg
     4561 ctcgcgcgcg gattcgccga cttttcggat tgcgcttgcg ccaggatttt caaattcgcc
     4621 agatcgtccc gtattttgcg ttgcattgcg atagtcagaa ccggttcgaa gactcggtcg
     4681 cttaaactta caaactgcgc gtgcgaactg aacaccaatc tcgtgtactc gccgttcgtg
     4741 atcagcttga aatttgcgtc gccggaaaaa ccctcggaaa cggaattacc ggaagaaatc
     4801 agcagcgata g
//
LOCUS       DASTPM010000753         4597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_200148, whole genome shotgun sequence.
ACCESSION   DASTPM010000753 DASTPM010000000
VERSION     DASTPM010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200148"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFR42_13295"
     CDS             complement(<1..212)
                     /locus_tag="VFR42_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370186.1"
                     /translation="MPALPKVEIRRATPAELPDAYLMVSEYYEAAAVQKRETQEEFLR
                     DYFCSGAGFWLARSGDNIIGCVALRH"
     gene            405..2969
                     /locus_tag="VFR42_13300"
     CDS             405..2969
                     /locus_tag="VFR42_13300"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU0370187.1"
                     /translation="MAQRTILEAERPTKSGKSGKRGANGAAAATPSLERERVLEAFRR
                     WGYLEASLDPLGLLEPLKSPDLDGFKGRDAEDARRIYAGTIGADFMHLLEPERRRWIA
                     ERMEGEIPETDRHMILSRLVRADLFEQVLQSRYLGTKRFSLEGVTSLIPLLDSILDAA
                     GEHGAIESVMAMSHRGRLNVMVHTACKTPHEVVAGFEDVDPRSVLGAGDVKYHVGATG
                     TYLTAHGKEIEIHLVSNPSHLEAVDPVAVGRSRAKLTRYAGGSTEFEANKVVPIVMHG
                     DAAFAGQGIFAETLNLADLKAYTVGGTIHIIVNNLIGFTTRPVQEHSSRFAADLAKRQ
                     DVPIFHVNAEDPDAVVWVGQIAADYRATFGSDVVVDLIGYRRHGHSEVDDPTITQPRL
                     YDKIKSHPPLWKLYAERIGVDAAKTVEAVRAEYEVEQTKAGKLKKIPHLRQLPHYWSA
                     FHRGKFKPEYEVDTGVPLETLAEITDKLVSIPAGFHVHPKIVKLLEQRAEMGHGKRAV
                     DYGFAEALAFGSLLQEGTPLRLTGQDSQRGTFNQRHAALVDTENEETYVPLAALAKES
                     EQQPFCEIFNSSLSEAGCLGFEYGFSRDYPEALVLWEAQFGDFVNGAQVIIDQFISAG
                     EDKWDLPSGIVMLLPHGFEGQGPEHSSARLERFLQLAAEENIQICQPSSASQYFHMLR
                     RQARRPWRKPLVVFTPKSMLRHPDASSSIEEFAQPRFLPVVPDREVTDARRILIASGK
                     VGHELKAERRKRKDTSTAILFLDQLYPLPRPEIAVALEAHSHAREVIWVQEEPRNMGA
                     FFYVMPRLTQLVQERGLSLRSVKRSSSASPATGSAKAHELEQKTLISLAFTTTSGD"
     gene            3031..>4597
                     /locus_tag="VFR42_13305"
     CDS             3031..>4597
                     /locus_tag="VFR42_13305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0370188.1"
                     /translation="MPFAPDTQLGPYKIAALIGAGGMGEVYRARDTRLLRDVALKVLQ
                     ESFTSDSDRLRRFEQEARAIAALNHPNIVSVYDVGVQDNVHYIVTELIEGDTLRRRIP
                     PQGISARKAIELAVQLANGLAAAHDQGIAHRDLKPENILITKDGRLKILDFGLAKLLP
                     SHLQTETLDGVTAAETNAGMVLGTAGYMSPEQVRGEPANHLSDIFSFGGILYEMLSGQ
                     RAFKKNTNAETMTAILNEEPQELASRIGAMPPALERIVRHCMEKQPKQRFQSARDIAF
                     DLESVSSTSGTSAIPAGGAKARARWVKPAIAAAAVLATGLALGMLVRPEAPELHPQLH
                     RITFSRLTILSARFTPDGNLIYGASLEGRPTELFFAQQGSVESRPIGMPSTNILAIAP
                     NGELAVQSAPVPTPSFAYRGTLARAPQTGGAPRPISEAVEYADWAPDGTFAAVRLVGG
                     KVRLEYPLGKVLYETAGWIGHPRISPDGTQVAFIDHPYARDDGGSVAIVDKTGQRKTL
                     TILSFVSAQGLAWH"
BASE COUNT          970 a         1249 c         1505 g          873 t
ORIGIN      
        1 atgtggcgca gagcgacgca gccgatgatg ttgtcgccgg agcgggcgag ccaaaatccc
       61 gcaccggaac aaaaataatc acgcaggaat tcttcctgag tctcgcgttt ttgaacggcg
      121 gcagcttcgt agtattccga gaccattagg taggcgtcgg gcagttcggc cggcgtggcg
      181 cggcgaatct cgaccttggg cagtgccggc attgggacaa ggctcgcaga gtgaaatggg
      241 tgtgcaactc ccattttgtc cggcggcggc tggccggctc gactcggctt tggctagtgg
      301 cacaaaaggc ggcggcggcc aggaagaaca ttcgttctgt ggcagaatta aaggttgggg
      361 ctgccgttgc gcggcatgct gcgaggcacg acaaggttag accgatggca cagcgaacga
      421 tcttagaagc ggaacgtcct acaaaatcag gaaaaagcgg aaagcgcggc gccaacgggg
      481 ccgcggctgc gacgccgtcg cttgagcgcg aacgagtgtt ggaagcattc cggcgatggg
      541 gctacctgga ggcgtcactc gacccactgg gattgctgga gccgctgaag tcgcccgatt
      601 tggacgggtt taaaggacgc gacgcggaag acgcgcggcg gatttacgcc gggacgattg
      661 gcgcggactt tatgcacttg ctggagccgg aacgccgacg gtggattgcg gagaggatgg
      721 aaggcgagat tccggaaacc gaccggcaca tgattttgtc gcgcctggtg cgtgcggatt
      781 tgtttgagca ggttttgcaa tcgcggtatt tgggcacgaa gcgattttcg ctggaaggcg
      841 tgacgtcgct gattccgctg ctggactcga ttctggatgc cgcgggcgag catggcgcga
      901 tcgaatcggt gatggcgatg agccatcgcg ggcggctgaa tgtgatggtg cacacggcgt
      961 gcaagacgcc ccacgaagtg gtagcggggt ttgaagatgt ggatccgcgc agcgtgctgg
     1021 gcgcgggcga cgtgaagtat cacgtcggcg cgacgggaac gtacctgacc gcacacggca
     1081 aagaaattga gattcacctg gtctcgaatc cgagccatct ggaagcggtg gatcccgtgg
     1141 cggtgggccg ttcgcgcgcg aagctgacgc gctacgccgg cgggtcgact gaattcgagg
     1201 caaacaaggt tgtgccgatt gtgatgcacg gcgacgcggc gttcgcgggc cagggaattt
     1261 ttgcggagac gctgaacctg gcggacttga aggcgtacac ggtcggtgga acgattcaca
     1321 ttatcgtgaa taacctcatc ggattcacga cgcggccggt gcaggagcac tcctcgcgtt
     1381 ttgcggcgga cttggccaag cggcaggacg tgccgatttt ccacgtgaac gcggaagatc
     1441 ccgacgcggt ggtgtgggtc ggccaaatcg ccgcggatta tcgcgcgacg tttggcagcg
     1501 acgtggtggt ggacctgatc ggttatcggc ggcacgggca cagcgaagta gacgatccga
     1561 ccatcacgca gccgcggctg tacgacaaaa tcaagagcca tccgccgttg tggaagcttt
     1621 atgctgagcg cattggtgtc gacgcagcga aaactgtgga ggctgtgcgg gccgagtatg
     1681 aggtcgagca gaccaaagcg gggaagttaa agaaaatccc gcacctgcgt caactgccac
     1741 attattggtc ggcgtttcat cgcggaaagt tcaaaccgga gtatgaagtg gacaccggtg
     1801 tccctctgga gacgctggcg gaaattacgg acaagctagt gagtattcca gctggcttcc
     1861 atgtccatcc gaaaatcgtg aaactgctgg aacagcgcgc ggaaatgggg cacggcaaac
     1921 gcgcggtaga ttacggattt gccgaggcgc tggcgtttgg ttcgttgttg caagaaggca
     1981 cgccgttgcg gctgaccgga caagactcgc aacgcggaac cttcaatcag cggcatgcgg
     2041 cattggtgga tacggaaaac gaagagacgt atgtgccact ggctgctctg gcgaaagagt
     2101 cggagcagca gccgttctgc gaaattttca attcgtcgct ttcggaagcg ggctgcctgg
     2161 gttttgaata cggcttcagc cgcgactatc cggaggcgct ggtgctgtgg gaagcgcagt
     2221 tcggcgactt tgtgaacggc gcgcaggtga tcatcgatca atttatcagc gcgggcgaag
     2281 acaagtggga tttgccgtca ggtattgtga tgttgctacc gcacggattc gaggggcagg
     2341 gtcccgaaca ttccagcgcg cggctggaac gattcctgca actcgcagcc gaagagaaca
     2401 tacagatttg ccagccatcc agcgcttcgc aatatttcca tatgctgagg cggcaagcac
     2461 gccggccgtg gcgcaagccg ctggtagtgt tcacgccgaa gagcatgttg cggcatccgg
     2521 acgcgtcatc gagcatcgaa gaatttgcgc aaccgcgatt tttgccggtg gtgccggacc
     2581 gcgaagtgac cgacgcgcga cgtattctga tcgccagcgg caaggttggg catgagttga
     2641 aagcggagcg ccgcaagcgc aaagatacca gcacggcgat cctcttcctg gaccagctat
     2701 atccgctgcc gcggccggaa attgcggtgg cgctggaagc gcattcgcac gcgcgcgaag
     2761 tcatctgggt gcaggaggag ccgcggaaca tgggcgcgtt tttttatgtc atgccgcggt
     2821 tgacacagtt ggtgcaagag cgcgggctga gcttgcgttc agtgaagcgg tcgtcgagcg
     2881 ccagcccggc gacgggatca gccaaggcac atgaactcga acaaaagacg ttgatttcgc
     2941 tggcatttac cacgacctcc ggcgactaag ccaacttacc ggttgcggat tgcggaatgc
     3001 gcccccagag gggatataat cccgccgtcc atgccctttg ccccagacac acaacttggt
     3061 ccatataaaa tcgctgcgtt aatcggcgcc ggcggaatgg gcgaggtgta ccgcgcccgc
     3121 gatacgcggc tgcttcgcga tgttgccctg aaagttctac aggaatcctt cacgagtgat
     3181 tccgatcggc tgcggcgatt cgaacaagaa gcgcgggcga tcgctgcgct aaaccatccc
     3241 aatattgttt ctgtgtatga cgtgggcgtt caggacaacg tccactacat tgttacggaa
     3301 ttgatcgaag gcgacacgct gcggcggcgc attcctccgc aggggatctc ggcacgcaaa
     3361 gctatcgaac tagcggttca gctggcaaat ggcctggccg ccgcgcatga ccagggaatc
     3421 gcgcaccgcg acctgaaacc ggaaaacatc ctcatcacca aggatggcag gttgaagatt
     3481 ctggattttg gcctcgcaaa gctgctgccg agccatttgc aaacggaaac tctggatggc
     3541 gtaacggcag ccgaaacgaa tgccggaatg gtgttgggca cggcagggta tatgtcgccg
     3601 gagcaggtgc gcggcgaacc tgcgaaccat ctttccgaca tttttagttt cggcggcatc
     3661 ttgtacgaga tgctgagcgg acagcgcgcg ttcaagaaga acacaaacgc ggaaacgatg
     3721 acggcgatcc tgaacgaaga accgcaggaa ctcgcctcgc gaatcggagc aatgccaccg
     3781 gcgctagagc gcattgtgcg gcactgcatg gagaagcagc cgaaacagcg tttccagtcc
     3841 gctcgcgata ttgcgttcga tctcgaatcg gtttcgtcaa catcgggcac atccgcgata
     3901 ccggccggcg gcgccaaggc ccgcgcacgc tgggtcaagc ccgcgatcgc tgcggcagcg
     3961 gttctcgcga ccggcctggc cttgggcatg ttggttcgtc ccgaggcgcc ggaacttcat
     4021 ccccagttgc atcgcatcac attcagccgc ctgacaatcc tgagtgcgcg cttcactccg
     4081 gacggaaatc taatctacgg cgcgtcactc gaagggcgcc cgactgaact cttttttgcg
     4141 cagcagggca gcgttgagtc acggccgatc ggaatgccct cgacaaatat ccttgccatc
     4201 gcgccaaatg gcgagttggc ggtgcagtcc gccccggtcc cgacgccgag tttcgcatac
     4261 cgcggcacgc tggcgcgcgc gccgcagacg ggcggcgctc cccgtccgat ttcggaagct
     4321 gtggaatacg ccgattgggc gccagatggt acgttcgcgg cagtccgtct tgtcggtggg
     4381 aaagtccggc tcgaatatcc gctgggcaaa gtgctctatg aaactgctgg gtggatcggc
     4441 catccccgca tttcgccaga tggaacgcag gtcgcgttca tcgaccatcc gtacgcccgc
     4501 gacgacgggg gctcggtcgc catcgtcgac aagacgggac agagaaaaac gttaacaatt
     4561 ctgagtttcg taagcgctca aggattggcc tggcatc
//
LOCUS       DASTPM010000754         9728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1353395, whole genome shotgun sequence.
ACCESSION   DASTPM010000754 DASTPM010000000
VERSION     DASTPM010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 9728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9728
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353395"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..1043)
                     /locus_tag="VFR42_13310"
     CDS             complement(312..1043)
                     /locus_tag="VFR42_13310"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEU0370189.1"
                     /translation="MEPSPTQPSLEDFRLRGVTITRLDVFSDVVLGIALVLLVVSLAI
                     PRSFSDLRSPTPSFTASAICFLMLVNVWFSHYVFFRRYGLCDRWTVLLNAALLGLVLF
                     CVYPLKFLYSTLFASLVRAELSPRFAAAIQINGMMVLYALGFTAVFFLVAALYWNAWR
                     QRDSLVLNGIERLLTVSSIVDALGVSAIGLIACLAALVLPTSWVLYSALLYFLLLPWK
                     ALNTIYFGRKARHLRSLIDPQPGLS"
     gene            complement(1138..2325)
                     /locus_tag="VFR42_13315"
     CDS             complement(1138..2325)
                     /locus_tag="VFR42_13315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0370190.1"
                     /translation="MAEIAIVGGGPSGSMCGEQLARAGHSVKLFDEHLAWEKPCGGGL
                     THKAIQRFPFLLENNYPKKLIHSIDLIASNEQRATLDMPHPIVIYARTVLNGMLLDRA
                     REAGCSIQRSHVVSVDTTSEKPRYCVEGEWHTADFLVLAAGARNQIFPETRPLKKDEL
                     EITQGYFIPQAEDSIIIKFLPHFDGYIWSFPRCDHLSVGICGSMASHNQSELRQHLQV
                     FIGKQKFAMDGARFYSHVLPSPQVPTLSQRTVVGKNWALVGDAAAFVDPLTGEGLFYA
                     MRSGELLGKAIAQGNPQIYTDWVRAAFSCELEFAARIVRRFYRGTFLGTAVTTRMVQF
                     LRRSSIFRQIMADIFSGTQDYTTLKQRVVGHLGMTLSDFVTSVLNVDTPVAKPRPGSV
                     PVP"
     gene            complement(2376..3893)
                     /locus_tag="VFR42_13320"
     CDS             complement(2376..3893)
                     /locus_tag="VFR42_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0370191.1"
                     /translation="MNRRLLLQAAAGLLLCALGYYLAHRESYDVHTVLASAGGCQMAS
                     DIYEPRSGTPVGAVVLFHGLAANKKVMAFNAQEFANLDLRVFVPDLPGHGRTPGPYSP
                     PRDDSCALSFVQDLAARKAIIPERTVLAGHSLGAAIAIRAASHFPVAGVIALSPAPMQ
                     TAPGFSAEMIPFHDVPALPPHSLLLTGQWEPGPIKALAQQLVATAADSSSKYQMIPAT
                     THVSILFSAQTFAQIRAWTSQILGTNPEAPFPKTMPALACMLGVIGLAILLPPFLREM
                     NVSRETDAPVGGPAELASIYLRGLWTTLAASFAVMLFLNPGYIPFRFVRVFQGDYLTV
                     FLFFSGIVALAATYKLLPPLAAFFNVPALSAAASAIVLTLLFAAWFELTFYEAWLNPA
                     RWLRFPVLFLLLLPAHLAEEIFLGSSRTSPNPARILKALALRAAVVAALLVGILFLHS
                     GEILFALLSAYFVVFSLLQRLACDLVRFRTQSSAAAAIFGAILLAAFALAIFPIA"
     gene            4004..4492
                     /gene="rfaE2"
                     /locus_tag="VFR42_13325"
     CDS             4004..4492
                     /gene="rfaE2"
                     /locus_tag="VFR42_13325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEU0370192.1"
                     /translation="MSGSVLKLHEAILRFAREKRNGRRVVFTNGCFDLLHPGHIETLE
                     KARNLGDALVVGVNSDRSVREMKGAGRPIVPERERAEMLAALECVDAVLIFDEPTPQE
                     IVAALLPDVLVKGGDWASDQIIGREEVEAAGGRVVSVPVVAGYSTSAMVEKIRKLSGM
                     ER"
     gene            4604..8035
                     /gene="mfd"
                     /locus_tag="VFR42_13330"
     CDS             4604..8035
                     /gene="mfd"
                     /locus_tag="VFR42_13330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU0370193.1"
                     /translation="MILPVVRERLEALVRQPALEGVVSALRGGAKPAGCTGLTELAKA
                     MAAAHVTHELRRPAFLLVESNKKAEAIAETLRFCFSVYPGPFAGTGGGVAVLPAFDTL
                     PWESRSPHADILERRAATLYRLAAGEVSLVIAPVGAALWRYRDAAQYADLTRVLEKDK
                     DVPLQEFLAHLGATGYARTEMVELPGQFAVRGGIVDIFSPEAPRPVRVELLGDTVESV
                     REFDPRTQRSIAPVNRTVVLPLTEWQVPRESAGEAWPLPTYWGPAGALGVRSLFELAA
                     SSLEPVIFVNEPTAVRTCVIDVLRDVTESYEKLGAANSPDGATFFWTEEEFELALQLA
                     TRVDLEQLGLVADEATRFHLASRPSARFHGDVVACVGDVKSQLSEGGTVFLTAASTGE
                     LERFADICREYEVPYVLGESESAAAGFTAESASESAGLVLVRAPFGEGTTFPDAKLTI
                     YGNADLFDVAPQVERPTRKIRTSGFFSDFADLKPGDFVVHVDHGIGQFEGLRQIESDG
                     RRGEFMLLRYAEDARLYVPLERMDLVQSYRVVEGAPPPLDKLGGTGWNTRKAKVRKSL
                     EDMAEQLLALYAARKTAQGFAFSSDGNWQREFEDAFEFEETQDQNLAIADIKKDLEKP
                     TPMDRLLCGDVGYGKTEVAMRAAFKVIGDGKQVAVLAPTTVLAFQHYETFKKRFAAFP
                     AKIEMLSRFRTAAEQKKVLAELEAGKVDVVIGTHRLLSKDVNFQDLGLLVIDEEQRFG
                     VAHKERLKEMRKNVDALSLSATPIPRTLHMSLVGLRDMSIIETPPRDRLAIQTAVSSF
                     SEELVRKAIETEMERDGQVYFIHNRVESIYSLAALVTKLVPKARVVVGHGQMGEKELE
                     QVMLKFIRDEADVLVATTIVENGLDIPRANTILINRADRLGLSELYQLRGRVGRSNQR
                     AYAYLLVPPQTVLSEVARKRLSAMKEFSELGAGFRIAALDLELRGAGNMLGRQQHGHI
                     EAVGFDIYTQMLERAVSKLKGEEAAPEMRTALSLGLDVRIPQDYIPSENLRLRTYKRI
                     SSIKTDNERTDVAKELEDRFGPLPPSVGNLLEYAALKSICERLRISMVERQGNRLAVR
                     FHPETLLDPATLVSVVRSRDGIRLDPSGVLWMELQRGEPVAGTLRNVLLGLQGQG"
     gene            8061..9335
                     /locus_tag="VFR42_13335"
     CDS             8061..9335
                     /locus_tag="VFR42_13335"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0370194.1"
                     /translation="MSNIFLKEPVQVVLGGALLALLLAGTPLVAQDPKQEPAPPAPTL
                     EQPKMAPAAEEPAKEAKPKPAPAPAVKPSANSGAVVEEIIARVNNEIITRSEYDKSLA
                     QTEEETRQDCQGKCTPEQLQSELDARKKTALRDLIDQSLLAQRGKDLGISVETDVVKQ
                     LDQIRIQNKLASMDELEKAVSSQGLNWEDFKNNIRNRILTQKVVSQEVGSHISIGESD
                     ARKYYDEHKSEFVRPEQVALREIIVNTEGKKDTELPDLKKKAETALKRVKDGEDFGEI
                     AKRLSDGTTKEQGGYIGTFKRGELAKQLEEVVFKMKHNELTDVIETKQGYLIFQVLEH
                     YDEGEQSFEKVKNEIMDRLYNTKLEPAVREYLKTLREQSYVVIKPGYAELAGSGNSEI
                     QEVNTTPEATKSKKGRKKFLLFGKRSGAASGQ"
     gene            9332..>9728
                     /locus_tag="VFR42_13340"
     CDS             9332..>9728
                     /locus_tag="VFR42_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEU0370195.1"
                     /translation="MKKTFVSELTSEQTITSFFLVSAKELRNTREGKPYLRLELCDRS
                     GTVEARMWTKFEIVAQEVAREDIVKVEARVELYRDRAQLALIQLRKARPDEIELADYL
                     PHTKEDVEKMWAELLAHGQSIGNSWLRTLA"
BASE COUNT         2230 a         2283 c         3484 g         1731 t
ORIGIN      
        1 gcgtggcgag ccatcgattt attcgagggc gataagatta atgaacgtgc tctaaaaaat
       61 ctcgtcctcg acgccgttga atacaatcat agtaaatcga agaggaaagc gcctgcggga
      121 actcgaacga aagtacataa aagtaagaag taacccagac tacagctttt tctcactcat
      181 tctttttgca agactggctg acatcccgga atcctgttcg agattagcca ggaacgccct
      241 ttggcatccc gcaatcgtgt tcagcttgtc tccggattcc tcaccgattc cggactttca
      301 cctgaagagc gttacgacaa gccgggttgc gggtcgatga ggctgcggag gtgacgggct
      361 ttgcgaccga agtagatcgt gttgagagct ttccagggga ggagcaggaa atagagaaga
      421 gcggaataga gaacccagga agtgggaaga acgagggcgg cgaggcaggc gatcaggccg
      481 atggcggata cgccgagtgc gtcgacgatg gaagagacgg tcaggaggcg ctcgatgcca
      541 ttgagtacga gcgaatctct ttggcgccag gcgttccaat agagagctgc aaccaggaaa
      601 aagacagcgg tgaacccgag ggcgtagaga accatcatgc cgttgatctg gatggcagcg
      661 gcgaagcggg gcgaaagctc ggcgcggaca agcgaggcga agagtgtgga atagagaaac
      721 ttcagaggat agacgcagaa gagcaccagg ccgagcagcg cggcgttgag gaggacggtc
      781 cagcggtcgc acaggccgta gcggcggaag aaaacgtagt gcgagaacca aacattgacc
      841 agcatcagga agcagatagc ggaagcagtg aaggacggag tgggagagcg gagatcggag
      901 aagcttctgg gaatggccag cgagacgacc aggagaacca gagcgatgcc caggacgacg
      961 tcgctgaaaa cgtcgaggcg ggtgatggtg acaccgcgga ggcggaaatc ttctagtgat
     1021 ggttgtgtgg gtgaaggttc caacggattt atccgaaaga aaatcaattc ctcagtggct
     1081 aaaaccccgt tgggcagcga acgatcgatg ctcggagcgg aagctccgac ccccaactta
     1141 agggacgggg acggagcctg ggcgcggttt ggcgaccggg gtgtccacgt tcaaaaccga
     1201 ggtgacgaag tcgctgaggg tcatgccgag gtggccgacg acacgctgct tgagagtggt
     1261 gtagtcctgc gtgccgctga aaatgtcggc catgatctgg cggaaaatgg agctgcgacg
     1321 caggaactgg accatgcgcg tggtgaccgc ggtgccgaga aacgtgccgc gatagaagcg
     1381 gcgaacgata cgggcagcga attccagttc acaggagaag gcagcgcgca cccaatcggt
     1441 gtagatctgg ggattgcctt gagcgatggc tttgccgagc agttcgccgg aacgcatggc
     1501 gtagaaaaga ccttcgccgg tgaggggatc gacgaaggcg gcagcgtctc cgacgagagc
     1561 ccagtttttg ccgacgacgg tgcgctgaga aagcgtaggg acctgagggg aaggcaggac
     1621 gtggctgtag aagcgggcgc cgtccatggc gaatttctgt ttgccgatga aaacctgcag
     1681 atgctggcga agttcggatt gattgtggga agccatgctg ccgcagatac cgacggataa
     1741 atggtcacag cgagggaagg accagatgta gccgtcgaaa tgaggcagaa acttgatgat
     1801 gatcgaatct tcggcttgcg ggatgaagta gccctgggtg atttcgagtt cgtctttttt
     1861 gagaggacgg gtttccggga agatctggtt gcgggcgccg gcggcgagaa ccaggaaatc
     1921 ggcggtgtgc cattcgccct cgacgcagta gcggggtttt tccgaagtag tgtcaacgga
     1981 aaccacatgc gaccgctgga tagagcagcc cgcttcgcga gcgcggtcga ggagcatgcc
     2041 gttcagaacc gtgcgcgcat agatgacgat gggatggggc atgtccagcg tggcgcgttg
     2101 ttcattgctg gcaatgagat cgatggagtg aatcagcttc ttggggtagt tgttttcgag
     2161 gagaaaagga aaccgctgga ttgccttgtg cgtgagacca ccgccgcaag gcttttccca
     2221 ggccagatgc tcgtcaaaaa gttttacgga atggccggcg cgagcaagct gttcgccgca
     2281 catggaaccg gacggaccgc caccgacgat tgcgatttca gccatggggt tttctcgctg
     2341 acctgagccg atttccgttt ctgagcgcga aagccttagg cgatggggaa aatcgccagg
     2401 gcgaaagcag cgaggagtat agcaccgaag attgcggcgg cggcagagga ctgtgtcctg
     2461 aaacgaacga ggtcgcaggc aagacgctgc agaagcgaga acaccacaaa gtacgcggaa
     2521 agaagagcga aaagaatttc accggaatga aggaagagga ttccgaccaa gagcgcggcg
     2581 acgaccgcgg cgcgtagggc aagcgccttg aggatgcgtg caggattggg agaagtgcgc
     2641 gaggaaccca ggaaaatttc ttccgccagg tgagccggga gaagcagcag gaaaagaacg
     2701 ggaaagcgca accagcgagc cgggttaagc caggcctcgt aaaaagtgag ttcgaaccag
     2761 gcggcgaaca gcagggtaag gacgatggcg gaagcggccg cggatagcgc tggaacgttg
     2821 aagaaagcgg ccaatggcgg gagcaatttg tacgtggcgg cgagagccac aattccagag
     2881 aaaaatagaa acacggtgag atagtcgccc tggaagacac gaacgaaacg aaacggtatg
     2941 tatccggggt tcaggaaaag catgaccgcg aagctggcgg cgagggtggt ccacaggccg
     3001 cgaagataga tggaggcaag ctcggcaggc ccgccaaccg gtgcgtcagt ttcgcgcgag
     3061 acgttcattt cgcgaaggaa cggcggaaga agaatcgcca ggccaatgac gccgagcatg
     3121 caggccagag cgggcatggt tttgggaaaa ggcgcttcag ggttggtgcc gaggatctgg
     3181 gaggtccaag cgcggatttg ggcaaaggtt tgggcggaaa agaggatgct gacgtgcgta
     3241 gtggcgggaa tcatctggta cttactggag gaatctgcag cggtggcgac gagttgctgc
     3301 gcaagcgcct tgatggggcc gggctcccat tgaccagtga ggagaaggga gtgtggcggg
     3361 agagcgggaa catcgtggaa aggaatcatc tcggcagaga agccgggagc ggtttgcatc
     3421 ggcgcgggag agagcgcgat gacgcccgct accggaaaat gcgatgcggc gcggatggcg
     3481 atggcggcac cgagcgagtg tccggcgagg acggtgcgtt cagggatgat ggctttgcgg
     3541 gcggcgagat cctggacgaa ggatagggcg caggagtcgt cgcggggtgg ggagtatggg
     3601 ccgggcgtgc gtccgtggcc ggggaggtcg ggaacgaaaa cgcgcaaatc caggttggca
     3661 aactcctgcg cgttgaaagc catgactttt ttgttcgccg cgaggccgtg aaagagaacg
     3721 acggcgccga cgggcgtgcc ggagcgcggt tcgtaaatat cgctggccat ctggcagccg
     3781 ccggcggagg cgagaaccgt gtgaacgtcg taggattcgc gatgggcgag gtagtagccg
     3841 agagcgcaaa ggagcaggcc agcggcggcc tgcagaagca ggcggcgatt catcgtaggg
     3901 ctggctgcca gagcgcaggg gctgaaagag gaccgtcgag ggccgcgtga taaactgaaa
     3961 ccgcgaaggc agtgaatgaa gcggggctga aaagggagtc cgattgagcg gcagcgtgtt
     4021 gaaattgcac gaagcgattc tccgatttgc gcgggagaaa cgcaatgggc ggcgggtggt
     4081 gttcaccaac ggttgtttcg atctgctgca tcccgggcat attgagacgc tggagaaggc
     4141 gcgaaatttg ggtgatgcgc tggtggttgg tgtcaacagc gatcgcagcg tgcgcgagat
     4201 gaaaggcgcg gggcggccga ttgtgccgga gcgggaacgt gcagaaatgc tggcggcgct
     4261 ggagtgcgtg gatgcggtgt tgatttttga tgagccgacg ccgcaggaga ttgtggcggc
     4321 gctgctgccg gatgtgctgg tgaagggcgg cgattgggcc agcgaccaga ttattggccg
     4381 cgaggaagtg gaggcggcgg gcggacgggt ggtttcggtg ccagtcgtgg ctggctattc
     4441 gacttcggcg atggtagaga agattcggaa gctgagtggc atggagcgat aaaagaaagc
     4501 gagattcctt ccgacaagag tcggaatttt acctccgcta cttcgctcgc ttcgctcgga
     4561 atgacaattt cagataagca aaacttttgc ctctaacgat tccatgatcc tgccggttgt
     4621 acgtgagcga ttggaagcac tggtgcgcca gcctgcgctg gagggagtgg tgtccgcgct
     4681 gcgcggcggc gcgaagccgg cggggtgtac ggggctgacg gagttggcca aggccatggc
     4741 ggccgcgcac gtgacccatg agttgcgaag gccggcgttt ctgctcgtgg agtccaacaa
     4801 aaaggcggaa gcgattgcgg agacgttgcg gttttgcttc tcggtttacc cggggccgtt
     4861 cgcagggacg ggcgggggcg tggcggtgtt gccggcgttc gacaccctgc cgtgggagtc
     4921 gcgcagtccg cacgcggaca ttctggaacg gcgggcggcg acgctatacc ggctggcggc
     4981 aggggaagtt tcgctggtga ttgcgccggt gggagcggcg ctgtggcgct accgcgatgc
     5041 ggcgcaatat gcggatttga cgcgcgtgtt ggagaaggac aaggacgtgc cattgcagga
     5101 atttctggcg catctgggcg ctacgggtta cgcgcgcacg gaaatggttg agctgccggg
     5161 gcaattcgcg gtgcgtggcg ggatcgtgga tattttttcg ccggaagcgc cgcggccggt
     5221 gcgcgtagag ctgctgggcg acacggtgga gtcggtgcgc gagtttgatc cgcgaacgca
     5281 gcggtcgatt gcgccagtga accgcacggt ggtactgccg ctgacggaat ggcaagtgcc
     5341 gcgcgagtcc gctggggagg cgtggccgct gccaacctac tgggggccgg cgggcgcgct
     5401 gggcgtgcga agtttgttcg aattggcggc cagttcgttg gagccggtga tttttgtgaa
     5461 cgagccgacg gcggtgcgaa cctgtgtgat tgatgtgctg cgcgacgtga ccgaatcgta
     5521 cgaaaaactg ggggcggcga attctccgga tggggcgaca tttttctgga cagaagagga
     5581 gtttgagctc gcgctgcagc tcgcgacacg cgtggacctt gagcagctgg gcttagtcgc
     5641 cgatgaagct acgcggtttc atctggcttc gcggccgagc gcgcggtttc acggcgatgt
     5701 agtagcttgc gtgggcgatg tgaagtcgca actgtcggag ggcgggacgg tttttctgac
     5761 cgcggcgagt acgggcgaac tggagcggtt cgcggacatt tgccgggaat acgaagtgcc
     5821 gtatgtgctg ggcgaatcgg aaagtgctgc ggcaggattt acggcggaga gtgcgagcga
     5881 gtcggcgggg ctggtgctgg tacgcgcgcc attcggcgaa ggcacaactt tccctgatgc
     5941 caagctgacg atttatggaa atgcggattt gtttgacgtg gcgccgcaag tggagcggcc
     6001 aacgcgcaag attcgcactt caggattttt cagcgacttt gcagacttga agccgggcga
     6061 ttttgtggtg cacgtggacc acggaattgg gcagttcgaa ggattgcggc agattgagtc
     6121 ggacgggcgg cgcggcgagt tcatgctgct gcgctacgcg gaagacgcgc gcttgtatgt
     6181 gccgctggag cgcatggacc tggtgcagag ctatcgggtg gtggagggag cgccacctcc
     6241 gctggacaag ctgggcggga ccggatggaa cacgcggaaa gcgaaagtgc gcaagtcgct
     6301 ggaggacatg gcagagcagt tgctggcgct gtatgccgcg cggaagacgg cgcagggatt
     6361 tgcgttttct tcggatggga attggcagcg cgaatttgaa gatgcctttg agtttgaaga
     6421 gacgcaggat cagaacctgg cgatcgcgga catcaagaag gatttggaga agcctacgcc
     6481 gatggaccgg ctgctgtgcg gcgacgtggg ttacggaaag acagaagtgg ccatgcgggc
     6541 cgcgttcaag gtgattggcg atggtaaaca ggtggcggtg ctggcgccga cgacggtgct
     6601 ggcatttcag cactacgaga cgttcaagaa aagattcgct gcgtttccgg cgaagatcga
     6661 gatgctgagc cggttccgca cggcggcaga gcagaaaaag gttttggcag agttggaagc
     6721 gggcaaagtc gatgtggtga ttggaacgca ccggctgctt tcgaaagatg tgaactttca
     6781 ggatttgggc ctgctggtga ttgacgaaga gcaacggttt ggcgtcgcgc ataaagagcg
     6841 gctgaaggag atgcgcaaga atgtggatgc actgtcgctt tcggcgacgc cgatcccgcg
     6901 cacgctgcac atgtcgctgg tgggattgcg agacatgtcg attatcgaga cgccgccgcg
     6961 cgaccggctg gcgattcaaa cggcggtttc atccttcagc gaagagttgg tacgcaaggc
     7021 catcgaaacg gagatggaac gcgacgggca ggtctatttc atccacaacc gcgtggaatc
     7081 gatttattcg ctggcggcgc tggtgaccaa gctggtgccg aaggcgcgcg tggttgtggg
     7141 acacgggcag atgggcgaaa aggaacttga gcaggtgatg ttgaaattta tccgcgacga
     7201 agcggatgtg ctggtggcga cgaccatcgt ggagaacggg ctggatattc cgagggcgaa
     7261 cacgattttg atcaatcgcg cggaccggct gggactttct gagttgtacc aattgcgcgg
     7321 gcgagtgggg cgttcgaatc agcgggcgta cgcgtatctg ctggtgccgc cgcagacggt
     7381 tttgtcggag gtggcgcgta agcggctttc ggcgatgaag gagttcagcg aactgggagc
     7441 ggggttccgc atcgccgcgc tggatttgga actgcgcgga gccgggaaca tgctgggcag
     7501 gcagcagcac gggcatatcg aggcggtcgg gttcgacatt tacacgcaga tgctggagcg
     7561 ggcggtttcg aagttgaagg gcgaagaagc agcgccggaa atgcggacgg ctttgagcct
     7621 ggggctcgat gtgcgaattc cgcaggacta tattcccagc gaaaacttgc ggctgcggac
     7681 gtacaagcgg atttcttcga ttaagacgga caacgagaga acggacgtgg ctaaggagtt
     7741 ggaggatcgg ttcggcccgc tgccgccgtc ggtgggaaac cttctggagt acgctgccct
     7801 caaatcgatc tgcgaacggt tgcgaatttc catggtggag cggcagggga accgcctggc
     7861 ggtgcgcttt catccggaga ctctgctgga cccagcaact ttggtgagtg tagtgcgttc
     7921 gcgggatggc attcgacttg atccgtcggg tgtgctgtgg atggaactgc agcgaggcga
     7981 gccggtggcc ggaacgctga gaaacgtatt gctcgggctt caagggcagg gctaaactga
     8041 aagcattgag aggcaaatga atgagtaata tttttcttaa agaaccggta caggtggttc
     8101 ttggcggagc ccttttggcg ctgttgttgg cggggacgcc gctggtggct caagatccga
     8161 agcaggagcc cgcgccgccg gcgcctaccc tggaacagcc gaagatggcg cctgccgccg
     8221 aagagccagc caaagaggcg aaacccaagc ccgcgccggc gccggcagtg aagccgagcg
     8281 caaacagtgg cgcggtggtg gaagagatca tcgcccgcgt aaacaacgaa atcatcacac
     8341 gttccgagta cgacaaatcg ttggcgcaaa ctgaagaaga aacccgccag gattgccaag
     8401 ggaaatgcac accggagcaa ctgcagagcg aactggacgc acgcaaaaag acggccttgc
     8461 gcgacctgat cgatcagtcg ctgctcgcgc agcgagggaa ggatctgggc atcagcgtgg
     8521 aaacggacgt ggtgaagcag ttggaccaga tccgcattca gaataaattg gccagcatgg
     8581 acgaactcga gaaagcggtc agcagccagg ggttgaactg ggaagatttc aagaacaaca
     8641 ttcgcaatcg gatcctgacg cagaaggtgg taagccagga agttggttcg cacatttcca
     8701 ttggcgagag cgatgcgcgc aagtattacg acgagcataa gtccgagttt gtgcggccgg
     8761 agcaggtggc gctgcgcgaa atcatcgtca acacggaggg gaagaaggac accgaactgc
     8821 ctgacctgaa gaagaaagcc gagaccgcgc taaagcgtgt gaaggacggc gaagactttg
     8881 gcgaaatcgc gaagcggctt tccgacggca ccacgaagga acagggcggg tacatcggga
     8941 cgttcaaacg cggcgaattg gccaaacagc tggaagaagt tgtcttcaag atgaagcaca
     9001 atgaacttac cgatgtgatt gagaccaagc agggatatct gatcttccag gtcctcgaac
     9061 attacgatga gggcgagcag tcctttgaaa aggtcaagaa tgagatcatg gaccgcctgt
     9121 acaacacgaa actggagccg gcagtgcgtg aatacctgaa gacgctgcgc gaacaaagtt
     9181 acgtggtgat caagccgggt tatgcggagc tggcggggag cggcaacagc gaaatccagg
     9241 aagtgaacac cacgccggaa gccaccaagt ccaaaaaggg gcgcaaaaag tttctgctgt
     9301 tcggcaaacg cagcggcgca gcttccggcc aatgaaaaag acgtttgtct ctgagctgac
     9361 gagcgaacaa accatcacca gcttttttct ggtaagcgcg aaagaactcc gcaacaccag
     9421 ggaaggcaag ccctatctga ggttggagct ttgcgaccgc tccggcacgg tggaagcccg
     9481 gatgtggacg aagttcgaga tcgttgccca ggaagtggcg cgcgaggaca tcgtcaaggt
     9541 agaagctcgc gttgaactgt atcgcgaccg cgcacagctt gcactgattc agctgcgcaa
     9601 agcgcggccc gacgaaatcg aactggccga ttatcttccg cacacgaagg aagacgtgga
     9661 gaagatgtgg gcggaattgc tggcgcatgg ccaatcgatc ggcaattcat ggttacggac
     9721 gctggctc
//
LOCUS       DASTPM010000755         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1209643, whole genome shotgun sequence.
ACCESSION   DASTPM010000755 DASTPM010000000
VERSION     DASTPM010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209643"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VFR42_13345"
     CDS             <1..1013
                     /locus_tag="VFR42_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0370196.1"
                     /translation="DTISGDEFVVHARKFVNATGTGADKVRTMANPLLAGRMRPSKGV
                     HLLFPANTFPGGDALLVPHTEDGRVIFAVPWQERLLVGTTDDEVTAATRMVVQKDEAE
                     YLLRQLNPYLQTPLRREQAVSGLAGLRPLVAAPGATATSKLIRDHEVELDSTTGLISI
                     LGGKWTTHRLMAQDTLDAVQRELGVTVSASLTRQQPLAGAEGYDTNFASKLEKEYALP
                     ADVARHLSGKFGARAIKIVELLRENPQWKERAAPGVPAIEAEFVYCIRNEMAETVEDL
                     LARRTGVQMYGWREALAAARVAAALLAQEKSWDAAKQEAAVAEYSGKIRGFLRELELH
                     EG"
     gene            1018..2520
                     /gene="glpK"
                     /locus_tag="VFR42_13350"
     CDS             1018..2520
                     /gene="glpK"
                     /locus_tag="VFR42_13350"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU0370197.1"
                     /translation="MKRYVGAIDQGTTSTRFMVFDGEGRVVSVAQKEHQQIYPRPGWV
                     EHDPAEIIARTTEVADEAIRRAGIAAAELVAVGITNQRETTIIWEKKTGKAIGNAIVW
                     QDTRVADDVARYAAAGGQNRFREATGLPLSTYFSSLKARWLLQNVAGARERAAAGELL
                     FGTVDSFLVWHLTGGTSGGVHVTDLTNASRTQLFNLSKLQWDPALLAAFEIPASLLPA
                     VRSSSEIYGEGTVGALKGVPIAGILGDQQAALVGQTCFDPGEVKNTYGTGCFLLMNTG
                     ERRVPSAAGLLTTVAYQFGKSPVHFALEGSIAISGALVQWLRDNLGLIAKSTDVEALA
                     RSVEDNGGVYFVPAFSGLYAPYWKDSARGVIAGLTRYANKGHLARAALEATAYQTRDV
                     VEAMEQDARIELKGVRVDGGMVGNELLMQFQSDILNREVVRPVVQETTALGAAYAAGL
                     ATGFYSGTEELRAKWKSDQVWTPQMASADREKLYRSWKKAVTRSFDWTEN"
     gene            2533..>3151
                     /locus_tag="VFR42_13355"
     CDS             2533..>3151
                     /locus_tag="VFR42_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEU0370198.1"
                     /translation="MHSAWLGEFLGTMILILLGNGVVAGVLLKKSKAEASGWMVIASG
                     WAFAVMAGVFTSIACGSSGAHLNPAVTLGFAVRAGSFHDVASFISAQVLGAMAGAALV
                     WMHYFPHWKETPDANAKLACFCTGPAIRSALPNFLSEVIGTFVLVFVVGAIFSKAVSA
                     SGPANALGPYLAGSLVWGVGLSLGGTTGYAINPARDFGPRLTHAIL"
BASE COUNT          666 a          829 c         1049 g          607 t
ORIGIN      
        1 gcgatacaat ttccggcgac gaattcgtgg ttcacgcgcg caaatttgtg aatgcgacgg
       61 gaaccggcgc ggacaaagtg cgcacgatgg ctaatcctct gctggcgggc agaatgcgtc
      121 ccagcaaggg cgtgcatttg ctgtttcccg ccaatacttt tccgggcggc gacgcattgc
      181 tggttcctca taccgaagac ggccgcgtga ttttcgccgt gccgtggcag gagcggctgc
      241 tcgtaggtac gaccgatgac gaagttactg ctgccacgcg aatggtcgtg cagaaagacg
      301 aagctgaata cttgttgcgc cagttgaacc cctatctgca aacgccgcta cgccgcgaac
      361 aggcagtgag cggattggca gggctacggc cactagtcgc cgcgccgggc gcgaccgcaa
      421 cgagcaagct gatccgcgat catgaagtgg aactcgatag cacgaccggt ctcatcagta
      481 tcttgggcgg aaagtggacg acgcaccggc tgatggcaca ggacacgctg gacgcggtgc
      541 aacgggaatt gggcgtgacc gtgagcgcgt cgttgacgcg gcagcagccg cttgccggcg
      601 cggagggcta cgacaccaat ttcgcaagca agctggagaa agagtatgct ctgccggcgg
      661 acgtggcgcg gcacttgtcg ggaaaattcg gcgcacgggc gatcaagatt gtggaattgc
      721 tgcgcgagaa cccgcaatgg aaagagcggg cggcgccggg cgttcccgcg atcgaggccg
      781 aattcgtata ttgcattcgc aacgaaatgg ccgaaacggt ggaagatttg ctggcgcggc
      841 ggacgggagt gcaaatgtac ggctggagag aagccttggc ggcggcgcgt gtcgcggcgg
      901 cgctgctggc gcaggaaaag agctgggacg cggcaaagca agaggctgct gtggcagagt
      961 actccggcaa gattcgcgga ttcttgcgcg aactggaact acacgagggc tagtcctttg
     1021 aaaagatatg ttggagcgat agaccaaggc accaccagca cccggttcat ggtgttcgat
     1081 ggagaggggc gcgtcgtcag cgtggcgcag aaggaacacc agcagattta tccgaggccg
     1141 gggtgggtgg agcacgaccc cgcggaaatc atcgcgcgga ctacggaagt tgcggacgaa
     1201 gcgattcgcc gcgcgggaat tgctgcggcg gaactggtgg cggtgggcat caccaatcag
     1261 cgcgaaacca ccatcatttg ggaaaagaaa accggcaaag ccatcgggaa tgcgattgtg
     1321 tggcaggaca cgcgagtggc ggacgatgtg gctcgttacg cggcggcggg cggacaaaat
     1381 cggtttcgtg aagcgaccgg actcccgcta agcacatatt tcagcagcct caaggcgcgc
     1441 tggctgctgc aaaatgtcgc aggcgcaaga gagagagccg cagcaggcga actgctgttt
     1501 ggcacggtag attcgttttt ggtctggcac ctgaccggcg gaaccagcgg cggagtgcat
     1561 gtgacggatc taaccaacgc cagtcgcacg cagctattca acctatcgaa attgcaatgg
     1621 gatccggcgc tgctcgcggc attcgagatt ccggcaagct tgctccctgc ggtgagatcg
     1681 agcagcgaaa tttacggcga agggacggtt ggcgcgttga aaggcgttcc catcgccgga
     1741 attctgggcg atcagcaggc agcgctcgtt ggacaaactt gtttcgatcc cggcgaagtg
     1801 aagaatactt acggcaccgg ctgctttttg ttaatgaaca ccggcgagcg cagggttcct
     1861 tccgctgcag gattgctgac cacggttgcc taccagttcg gcaaatcgcc ggtgcacttt
     1921 gcgctggaag gcagcattgc tatcagcggc gctttggtgc aatggctgcg cgacaacctg
     1981 ggcctgatcg cgaagagtac tgacgtagaa gcgctggcgc gcagtgtcga ggataatggc
     2041 ggcgtctact ttgtgccggc gttcagcggg ctctacgcgc cgtattggaa agattccgcc
     2101 cgcggcgtga ttgcgggttt gacgcgctac gccaacaaag gccacctggc tcgcgccgcg
     2161 ctcgaagcca ctgcttacca gacgcgcgac gtcgtggaag cgatggaaca ggatgcgagg
     2221 atcgaactga aaggcgtgcg cgtggacggc ggcatggtcg gcaacgaact gctgatgcaa
     2281 tttcaatcgg atattttgaa ccgtgaagtg gtgcgcccgg tggttcagga aaccacagct
     2341 ctgggtgcgg cttatgccgc gggcctggca accggttttt attcgggaac cgaagagcta
     2401 cgcgctaaat ggaaatcgga tcaagtttgg acgccacaaa tggcaagcgc ggatcgcgaa
     2461 aagctctacc ggagctggaa aaaagcagtc acacgttcgt tcgactggac cgaaaactga
     2521 cggaggaatg aaatgcactc agcttggctg ggcgaatttc tgggcacgat gatcttgatt
     2581 ctgctgggca acggagttgt cgccggtgtg ctactgaaaa aatcgaaagc tgaggccagc
     2641 ggctggatgg tcatcgcttc gggttgggct ttcgccgtaa tggcaggagt gtttacatcg
     2701 atcgcctgcg gaagcagcgg agcacacctg aatccggcgg taacactggg ctttgccgtg
     2761 cgtgccggaa gtttccacga tgtagcgtca ttcatatccg cgcaagtgct gggcgcgatg
     2821 gccggcgccg cgctggtttg gatgcactat tttccgcatt ggaaagaaac accggacgcc
     2881 aatgcgaagc tggcctgctt ttgcaccgga cctgcaattc gcagcgcttt gccgaatttt
     2941 ttgagtgaag tgatcggcac gttcgtgctc gtattcgtgg tgggtgctat tttttccaag
     3001 gccgtctccg ccagcggacc cgccaacgcg ttggggccgt atctcgccgg gagcctggtc
     3061 tggggtgttg ggttgtcgct gggcggcacg actggttacg cgatcaatcc cgcacgcgac
     3121 tttggaccgc gtctgactca tgccatcctt c
//
LOCUS       DASTPM010000756         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1411476, whole genome shotgun sequence.
ACCESSION   DASTPM010000756 DASTPM010000000
VERSION     DASTPM010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411476"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /gene="glgB"
                     /locus_tag="VFR42_13360"
     CDS             <1..1597
                     /gene="glgB"
                     /locus_tag="VFR42_13360"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU0370199.1"
                     /translation="KADPYGFRSELRPNTGSVVANLQTHAWNDSAWLAERAKKDWLHS
                     PITVYEVHLGSWKRVPEDQNRWLTYRELADQLIPYVKDLGYTHIELLPIMEHPYDGSW
                     GYQTLGYYAATSRYGSPTDFMEFVDRCHQAGIGVLLDWTPAHFPRDAHGLAQFDGTHL
                     FEHADPRQGSQPDWGTLVYNYGRNEVQNFLISNALFWLDKYHIDGLRVDAVASMLYLD
                     YSRKEGEWIPNRFGGRENLDAISFFKRLNEEAHGRFPGILTVAEESTAWPSVSRPVYL
                     GGLGFSLKWNMGWMNDTLKYFSSNPIYRKYEHNKITFSLIYAFSENFMLPFSHDEVVH
                     GKNSLLHKMPGDMWQQFANLRLLLAYQYAHPGKKLLFMGQEIAQRNEWSEARSLDWHL
                     LQWDSHQGVQQLVRDLNRLVAAEPALHQVDFEWQGFEWIDCNDSDDSVLSFVRTGRNS
                     DDLIVVVLNATPVVREGYIVGVPRAGYYKEVLNTDAGVYGGSNVGNIGGQLAANDPRQ
                     GRPFSLCLTLPPLAAIFLKFTPA"
     gene            1725..>2106
                     /locus_tag="VFR42_13365"
     CDS             1725..>2106
                     /locus_tag="VFR42_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370200.1"
                     /translation="MKKFILRLGFSALGVAATLLWWTYHNTGSSAQSVSHIPAKVGQG
                     GNQLVVDAETTTPSTLRLNFEDLRKPAGQQILLESWEKIPAGPHSWTVDIPSGIGGYI
                     ELEADHPNPGDTLTQRIRINGREVD"
BASE COUNT          457 a          672 c          533 g          444 t
ORIGIN      
        1 gaaagccgat ccctacggct ttcgctccga actccggccg aacaccggtt cggtcgtcgc
       61 gaatctccaa acccatgcgt ggaacgattc cgcctggctc gccgagcgcg ccaaaaaaga
      121 ctggctccat tcgccgatta ctgtctacga agtccatctc ggctcctgga agcgcgttcc
      181 cgaagatcaa aaccgctggc tcacgtatcg cgaactcgcc gatcagctca ttccctatgt
      241 aaaagatctc ggctacaccc acatcgaatt gctcccgatc atggagcatc cctatgatgg
      301 ctcgtggggc tatcaaaccc tcggctacta cgccgcaacc agccgctacg gctcacctac
      361 cgacttcatg gagtttgtcg atcgctgcca tcaagccgga atcggcgtgc tactcgattg
      421 gactccagcg cacttcccgc gcgacgcgca cggcctggcg caattcgacg gcacgcactt
      481 gttcgaacac gcggacccgc gccagggctc gcagcccgat tggggcacgc tggtttacaa
      541 ctacggccgc aacgaagtgc agaatttcct catttccaac gcccttttct ggctcgataa
      601 atatcacatc gatggactgc gtgtggacgc cgtcgcctcc atgctctatc tcgattattc
      661 gcgcaaagag ggtgaatgga ttcccaatcg tttcggcggc cgcgaaaatc tggacgcgat
      721 ttcctttttc aagcgcctca acgaagaagc gcacggacgt ttccccggca tcctcaccgt
      781 ggccgaagaa tccactgcct ggcccagcgt gtcgcggccg gtttacctcg gcggcctcgg
      841 cttcagcctg aaatggaaca tgggttggat gaacgacacg ctcaaatatt tttcctccaa
      901 cccgatttat cgcaaatacg agcacaacaa aatcacgttt tctttgatct acgcgttcag
      961 cgaaaacttc atgctgccct tttcgcacga tgaagtcgtg cacggcaaaa attccctgct
     1021 gcacaaaatg ccgggcgata tgtggcagca attcgccaac ctgcgcttgc tgctggcata
     1081 ccaatacgca catcccggca aaaaactgct atttatgggc caggaaatcg cgcaacgcaa
     1141 cgaatggagc gaagcgcgca gcttggactg gcacttgctg caatgggatt cgcaccaagg
     1201 cgtgcagcag ttggtccgcg acctgaatcg cctggtcgcc gcggagcccg ccctgcacca
     1261 ggtcgatttt gaatggcagg gcttcgaatg gatcgattgc aacgacagcg acgatagcgt
     1321 gctttccttc gtccgcacgg ggaggaactc agacgatctg atcgtcgtcg tgctcaacgc
     1381 cacgccggtg gtgcgcgaag gctacatcgt cggcgtgccc cgcgccggtt attacaaaga
     1441 agtgctcaac accgacgccg gagtctacgg cggatcgaac gtcggaaaca tcggcggcca
     1501 gctcgcggcg aacgacccgc gccaaggccg gccgttttct ctttgcctca ctttgccgcc
     1561 ccttgcggcc atcttcctca aattcacgcc ggcttgaatt aggctcgccc gttccagcga
     1621 tatcacccct ttctttctgg aaacaagctc cgccttttac tcatgtcagt tgtgtccagg
     1681 tcatacactg actgcaaggc tgttcaattc tgacggagcc caccatgaaa aaattcattt
     1741 tgcgattggg tttctctgct ttaggcgttg ccgccacgct gctgtggtgg acttatcaca
     1801 acaccggttc cagtgctcaa tcggtgagcc acattcccgc aaaagtcggc caaggcggca
     1861 accagttggt cgtcgatgcc gaaacgacca cgccttcaac tctgcgcctc aatttcgagg
     1921 atcttcgcaa acccgcaggc caacaaatcc tgctcgagtc ctgggagaaa attcccgccg
     1981 gtccgcacag ttggaccgtt gatattccca gcggcattgg cggctacatt gaactcgaag
     2041 ccgatcaccc caatccgggc gacaccctca cccaacgcat ccgcatcaat gggagagagg
     2101 tcgacc
//
LOCUS       DASTPM010000757         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_27345, whole genome shotgun sequence.
ACCESSION   DASTPM010000757 DASTPM010000000
VERSION     DASTPM010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27345"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFR42_13370"
     CDS             complement(<1..466)
                     /locus_tag="VFR42_13370"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0370201.1"
                     /translation="MSNRLAGKVAAVTGGDQGIGRAIAERLAQEGADVAICYRKNKDG
                     ADEVVGRIKSAKRRAAAFQADVGKVSDGQRFIEQALAALGKVDILVNNAGLEKRADFW
                     DVTEEDFDAVLNVNLKGMFFITQALVRHLRQTGRPGKIINISSVHEELPFPHF"
     gene            complement(537..1613)
                     /locus_tag="VFR42_13375"
     CDS             complement(537..1613)
                     /locus_tag="VFR42_13375"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEU0370202.1"
                     /translation="MATTPRTTIQSLLGAKAESLLGFHSPKIAKERLHLPGPDFIDRV
                     WLGSDRNTRVLTNLERIYEHGRLGKTGYLSIFPVDQGIEHSAGASFAKNPDYFDAENI
                     VKLAIEGGCNAVASTCGVLGAVARKYAHKIPFIMKLNHNELLTYPNKFDQVFFSTVKL
                     AYDMGAAAVGATIYFGSEQSDRQIVEVSQAFAHAHELGMATILWCYLRNNTFKKDKDY
                     HLSADLTGQANHLGVTIQADIIKQKLPENNGGYQALNMGDSSYGKFDKRIYSELASDH
                     PIDLCRYQVANCFMGRAGLINSGGASGANDFEEAVATAVINKRAGGTGLISGRKAFQR
                     PLAEGIQLLNTIQDVYLSPEVTVA"
     gene            complement(1623..2366)
                     /gene="pgl"
                     /locus_tag="VFR42_13380"
     CDS             complement(1623..2366)
                     /gene="pgl"
                     /locus_tag="VFR42_13380"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU0370203.1"
                     /translation="MADANAIAQTAAAEFLEAAKQAVREKGNFCVALSGGSTPKALYG
                     LLISNPILQAMVPWGKIQFYFGDERHVPPDDTESNFRMATEAMLAKAPVDAQQVHRIK
                     GEKRNAAQAAEEYEEDLRTSFRLAEGRLPNFDLVLLGMGPEGHTASLFPGTKALKEQR
                     RLVVSNWVGKLYTDRITLTPPVLNNAAHIIFMVQGAEKAPALKAVLEGPYEPDQLPAQ
                     IIKPKQGKVLWLVDPTAAAMLAPQAKRAF"
     gene            complement(2413..>3442)
                     /gene="zwf"
                     /locus_tag="VFR42_13385"
     CDS             complement(2413..>3442)
                     /gene="zwf"
                     /locus_tag="VFR42_13385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU0370204.1"
                     /translation="IYRIDHYLGKETVQNLMVFRFANSIAEPLWNRNFIDSVQITAAE
                     TVGVEQRGGYYETSGALRDMVPNHLFQLLSLTAMEPPISFDADAVRDKQAEILHAVQV
                     SKPEDVMSMAVRGQYGQGATGAERLLAYRTEPMVSAVSNTETYAALRLEIDNWRWAGV
                     PFYLRTGKRLSRRTTEIAIQFRRSPFVLFRNTKIADLQTNRLVIHIQPDERISLRFGA
                     KVPGPVMELGLVNMEFDYARDFGRSHNTGYERLLYDCMMGDATLFQRADMVEAGWRVI
                     QPVLDAWSAAPAKCFPNYAAGSWGPAESDELLARDGRAWRPIGEEHPPSEPSEVPAAL
                     GQATAGAH"
BASE COUNT          673 a         1040 c          922 g          807 t
ORIGIN      
        1 caaaatgcgg aaacggcaat tcttcgtgca ccgaactgat gttaatgatt ttcccggggc
       61 gcccggtctg ccggagatgg cggaccaggg cttgcgtgat gaaaaacatt cccttcaggt
      121 tgacgttcaa gacggcgtcg aagtcttctt ccgtcacgtc ccagaagtca gctcgtttct
      181 ccaggcccgc gttgttgacc aggatgtcga cttttccaag ggccgcgagg gcctgttcaa
      241 tgaatcgctg tccgtcagaa acctttccca catcggcttg aaacgcagca gctcgccgct
      301 tcgcagattt aattcggccc actacttcgt ctgccccgtc cttgttcttg cgatagcaga
      361 tggccacgtc ggccccctcc tgcgccaatc gttccgcaat cgcccgtcca attccctggt
      421 cgccgcccgt tactgcggct actttgcctg ccagccgatt tgacaacact cctcctcgcg
      481 gttcttcttg tcttgaaaaa gaacccattg caaattcgtt ttagcctctt aaatcctcag
      541 gccaccgtga cttccggact caaatagacg tcctgaatgg tgttcagcaa ttgaatccct
      601 tccgcaagcg gccgttgaaa tgccttccgg cccgagatca gcccggttcc gcctgccctc
      661 ttgttgatca ccgccgtggc tactgcctcc tcgaagtcat tcgcgccaga cgcccctccg
      721 ctgttgatca aaccggctcg tcccatgaaa caattcgcca cctggtagcg gcacagatca
      781 atcggatgat cgctcgcaag ttccgagtag atgcgcttgt cgaacttgcc gtagctcgaa
      841 tcgcccatgt tgagcgcctg gtagccgccg ttgttctcgg gaagcttttg cttgatgatg
      901 tccgcctgga tggttacacc cagatgattg gcctgtccgg tcaaatccgc cgagaggtgg
      961 tagtccttgt ctttcttgaa ggtattgttg cgcaggtaac accacagaat ggtggccatt
     1021 cccagttcgt gcgcgtgcgc gaaggcttgg ctcacttcca cgatctgccg gtcactctgc
     1081 tccgagccaa agtagatcgt cgcccctacc gcagctgccc ccatgtcgta cgccaacttc
     1141 accgttgaga agaatacttg atcgaacttg ttcgggtagg tcagcaattc gttgtgattc
     1201 aacttcatga tgaagggaat tttgtgtgcg tacttgcgcg cgactgctcc cagcacgcca
     1261 caagtcgaag ccactgcgtt gcatccgcct tcgatagcca gctttacgat gttttccgca
     1321 tcgaagtaat cgggattttt cgcaaagctg gcgcccgcgg agtgctcgat gccctggtcg
     1381 acgggaaata tcgaaagata cccggtcttc cccaggcgcc catgttcgta gatcctttct
     1441 aagttcgtca gaacgcgagt gttcctgtcg gatccaagcc aaacgcgatc tatgaaatcg
     1501 gggccgggaa gatggaggcg ctccttggca atctttggtg aatggaatcc cagcaaggat
     1561 tccgctttcg ctcccagaag ggattgaatt gtcgtacgcg gagttgtagc catggttttc
     1621 tcctagaatg ctctcttcgc ctgcggagca agcatggcgg ccgccgtggg atccacgagc
     1681 cacaacacct tgccctgttt tggcttgata atctgcgcgg ggagttggtc cggctcgtac
     1741 ggtccctcga gcacggcctt tagtgccggc gccttttccg ccccctgcac catgaaaatt
     1801 atgtgggctg cgttgttcaa caccggaggc gtcagcgtga tgcgatccgt atagagcttg
     1861 cccacccaat tgctaaccac cagacggcgt tgttccttca gggcttttgt gcctggaaat
     1921 aaagaagccg tatgcccttc cggccccatc ccgagcagca ccaagtcgaa gttcggcaac
     1981 cgcccttcag ctagcctgaa actggttcgc aaatcttctt catactcttc tgccgcttgc
     2041 gccgcgtttc gtttttcacc tttgatacga tgcacctgct gcgcatcgac tggggcttta
     2101 gcaagcatcg cctccgtggc cattcgaaaa ttgctttctg tgtcatctgg aggtacatgc
     2161 cgttcgtcgc cgaaataaaa ctggatcttg ccccagggca ccattgcctg caggatcggg
     2221 ttgctgatca gcaacccgta cagcgctttc ggtgtggagc cacccgagag cgcgacgcag
     2281 aagttccctt tttcgcgaac ggcctgcttt gctgcttcaa ggaattccgc tgcagccgtt
     2341 tgcgcaatgg cattggcatc cgccaaaatt cgaatctcac ggtttgcggc tagagtttca
     2401 gtcgtcatag tcttagtgcg ctcctgcagt cgcctgcccc agcgccgcag gaacctcact
     2461 gggctcagac ggcggatgct cttccccgat cggtcgccac gcacgtccgt cgcgcgcaag
     2521 caattcgtca gattcggccg gcccccagct tcccgcggca tagttcggaa aacacttggc
     2581 cggagcggcc gaccatgcat ccagaaccgg ttgaatcact cgccagcccg cttctaccat
     2641 gtccgcgcgc tggaacaggg tggcgtcgcc catcatgcag tcgtacaaga gccgttcgta
     2701 cccggtgtta tgggagcgcc cgaagtcgcg tgcgtaatca aactccatgt tcaccaggcc
     2761 gagttccatc accggaccgg gaaccttagc tccgaatctc agcgaaattc gttcgtctgg
     2821 ctggatgtga attaccaacc ggttcgtctg cagatccgcg atcttagtat tgcgaaacaa
     2881 aacgaaggga gaacggcgaa actgaatggc gatttcggtc gtgcggcgcg agaggcgctt
     2941 acctgtacgg aggtagaacg gcacgccggc ccaccgccag ttatcgattt ccagccgcaa
     3001 cgcggcatag gtctcggtat tcgaaacggc ggaaaccatg ggctcggtgc gataggcgag
     3061 cagccgttcg gctcctgtcg ctccctggcc gtattgtccg cgaaccgcca tgctcatcac
     3121 gtcttctggc ttagagactt gaacggcatg gaggatctcc gcttgcttgt cgcgcacggc
     3181 gtctgcgtca aacgaaatgg gtggctccat ggccgtcagc gaaagcaact ggaagagatg
     3241 attaggaacc atgtcgcgca gggccccgga cgtctcgtaa tagccgccgc gctgctcgac
     3301 gccgaccgtc tcagcggccg taatctgcac gctatcgatg aagtttcggt tccacaaggg
     3361 ctcggcgatg ctgttcgcaa aacggaagac catgaggttc tgcacggtct ctttgcccag
     3421 atagtgatcg atgcggtaga tc
//
LOCUS       DASTPM010000758         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1180976, whole genome shotgun sequence.
ACCESSION   DASTPM010000758 DASTPM010000000
VERSION     DASTPM010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180976"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFR42_13390"
     CDS             <1..406
                     /locus_tag="VFR42_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370205.1"
                     /translation="AKYCSGFYGPFREAADSAPQFGDRRSYQMDPGNAREALKEVALD
                     LGEGADMVMVKPALPYLDIVRQVKDEFRVPVAAYNVSGEYSMVKAAAKNGWLDEKRVV
                     LEILTGIQRAGADFILTYHAKDAARWLRSAER"
     gene            453..1763
                     /gene="hemL"
                     /locus_tag="VFR42_13395"
     CDS             453..1763
                     /gene="hemL"
                     /locus_tag="VFR42_13395"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU0370206.1"
                     /translation="MSSTPSPQHGHRRSREIFAEAEQILVGGVNSPVRAFRSVGGEPL
                     IIERGQGQYLFDADGNRLLDFVGSWGAMLLGHAHPAVVAAIADQAQKGTSYGVTTELE
                     VQLAQRMRRAVPFLEKIRFVSSGTEATMSALRLARGVTGRDLVIKFEGCYHGHADSFL
                     SQAGSGLATLGIAECPGVPQALAELTLNVPYNDLAAVENRFAQQEGKIAAVIVEPVAA
                     NMGVVLPQKDFLPGLRDLTKKHGALLIVDEVITGFRVRNGSCQPNYGIEADLTTFGKI
                     IGGGLPVAAYGGPSNIMDRVAPLGPVYQAGTLSGNPLAMRAGIATLEQLSKPGLYERM
                     EKLAAAFVSGLREILRAQKIPAQVNSVGSLATLFFTSEPVVNYTAAKKSDTKRYAEYF
                     RSMLDRGVFLAPSQFEASFVSAAHTDLDIEFALKSAKESLKQPV"
     gene            1874..2413
                     /locus_tag="VFR42_13400"
     CDS             1874..2413
                     /locus_tag="VFR42_13400"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEU0370207.1"
                     /translation="MHELEGHGGHGGEHSGPLIIPVSITISILAVLVAAVTLLGHRAH
                     TEELLLQAKASDQWAYYQAKNIRLRELQSFADLLGVVTTQDKEKTAEVREKYAKEIER
                     YGGDKEDISKEAKDQEHERDLYQRRADHFDAAEGVLEFALIICSLTLLTNKKFFWFSG
                     IAIGIAGVIGALTGFLIRV"
     gene            complement(2464..3060)
                     /locus_tag="VFR42_13405"
     CDS             complement(2464..3060)
                     /locus_tag="VFR42_13405"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEU0370208.1"
                     /translation="MNAMHDMWKDMFVLALPIAEKILRPIVVYGFLVISLRLSGKREL
                     VQLNPFDLVVLLTLSNTVQNAIIGDDNTVTGGIIGAASLLLVNYLVVRFLYNHRALDQ
                     LIEGKADILIEDGKVRTQHLKRELITMAQLEAAARKQGFESLSEVQQCVLEAGGTLTF
                     LGKKPGTDEARHRELLQRFDALSAELARLRGAEPPLKA"
     gene            3370..>3644
                     /locus_tag="VFR42_13410"
     CDS             3370..>3644
                     /locus_tag="VFR42_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0370209.1"
                     /translation="MSKSWLLAPDAACTEFQARALLAATRELLAIVSASGQFLFVNDN
                     FSRVLGYSPQELVGKSFLSLHSAADAALIQQKFSCAVSARNDDTVSCR"
BASE COUNT          761 a         1125 c          968 g          790 t
ORIGIN      
        1 cgcaaaatac tgttccggct tctacgggcc ctttcgcgag gccgctgact cagctccaca
       61 attcggcgat cgccggagtt atcagatgga tccgggcaat gctcgcgaag cgttgaaaga
      121 ggtcgcgctc gatctcggag agggcgccga catggtcatg gtcaagcccg ctcttccgta
      181 tctcgacatc gtgcggcaag tgaaagacga atttcgcgtt ccggtggccg cttacaacgt
      241 cagcggcgaa tattccatgg taaaagccgc ggccaagaat ggctggctcg acgaaaaacg
      301 ggttgttctt gaaattctca ccggcatcca gcgcgccggc gccgacttca ttttgacgta
      361 tcatgcaaag gacgctgccc gctggctgag gtccgcggaa agatgattcg acttgccaat
      421 cacgatccga taacggccga accagagcac agatgagctc cacgccctcg cctcaacacg
      481 gacaccgccg ctcgcgcgaa atcttcgcgg aagccgaaca aattctcgtc ggcggcgtca
      541 acagtccggt gcgcgctttt cgctccgtcg gtggcgaacc gctgatcatt gaacgcggcc
      601 aaggccaata tctttttgat gccgacggta accggttgct cgatttcgtc ggctcctggg
      661 gagctatgct gctcgggcat gcgcatcccg ccgtcgtcgc cgccatcgcc gatcaggcgc
      721 agaagggaac tagctacggc gtcaccaccg aacttgaagt tcaactcgcg caacgtatgc
      781 gacgcgccgt cccgttcctg gaaaagattc gcttcgtttc gagcggcacc gaagccacca
      841 tgagtgccct ccgcctcgcc cgtggcgtca cgggacgcga cctggtcatc aaatttgagg
      901 gctgttatca cggccatgcc gacagctttc tttcgcaagc cggctcgggc ctcgccacgc
      961 tcggcatcgc tgaatgcccc ggcgtgccac aagcgctcgc cgaactcacg ctcaacgttc
     1021 cctacaatga tcttgccgcc gtcgaaaatc gttttgcaca acaagaaggc aaaattgctg
     1081 ctgtcatcgt cgagcctgtc gcggcgaata tgggcgtagt ccttccacaa aaagattttc
     1141 ttccgggctt gcgcgacctc acgaaaaaac acggggcatt gctcatcgtg gacgaagtca
     1201 tcaccggctt ccgcgtccgc aacggttctt gccagcccaa ttacggcatc gaagccgatc
     1261 tcacgacctt cggcaaaatt atcggcggcg gattgcccgt cgcagcatac ggcggcccgt
     1321 ctaacatcat ggaccgtgtc gcgcccctgg gccccgtcta ccaggccggc actctgtcgg
     1381 gtaatccgct cgccatgagg gccggcatcg ctactctcga gcagctcagc aaacctggcc
     1441 tctacgaacg catggaaaaa ctcgccgccg ctttcgtgtc gggcctgcgc gagatcctcc
     1501 gcgcgcagaa aatcccggcc caggtgaatt ccgttggctc tcttgccacg ctctttttca
     1561 cgagtgagcc tgtcgtgaac tacaccgccg caaaaaaatc cgacaccaaa cggtatgccg
     1621 aatatttccg cagcatgctc gaccgtggtg ttttcctcgc tccctcccag ttcgaagcat
     1681 cctttgtctc cgccgcgcac accgacctcg atatcgaatt tgccctcaag tctgccaaag
     1741 agagcctgaa gcagcccgtc tagcctcgtt gggcacccaa gaagcaggta atctcctttc
     1801 tcgcctgaca aacacttgag cctgcgaact gtgcgaaact actcccacaa aaattcctgg
     1861 aggcgtcact cccatgcacg aactagaagg ccacggcggt cacggaggcg agcatagcgg
     1921 cccgctgatc attcccgtct cgatcaccat ttccatcctc gcggttctgg tggccgctgt
     1981 cacacttctc ggccaccgtg cccacaccga agagcttctt ctgcaagcca aggccagcga
     2041 ccagtgggct tactatcagg cgaagaacat acgcctgcgc gagctgcaaa gctttgccga
     2101 tttactggga gtggtcacca cgcaggacaa agagaaaacc gccgaagttc gcgagaaata
     2161 tgcaaaggaa attgagcgct acggcggcga taaggaagac atcagcaagg aagccaaaga
     2221 tcaggagcac gagcgcgatc tgtaccagag acgcgccgac cactttgatg ctgccgaagg
     2281 cgtccttgaa ttcgcgctga tcatttgctc gctgacacta ctcaccaaca aaaagttctt
     2341 ttggttctcc ggcatagcca tcggtatcgc gggcgtgatt ggcgcgctca cgggtttcct
     2401 gatccgcgtt taaaacgtta gatggtgctc gttagtatcg taagtggcgt gggcttatct
     2461 gctctaggct ttcaggggag gctccgctcc gcgcaagcgc gccaactctg cggatagcgc
     2521 atcgaaccgc tgcaacagct cacgatggcg agcctcgtcc gttccgggtt ttttgccgag
     2581 gaacgtaagc gttccgccgg cctctagcac gcactgctgc acttcgctga gggactcaaa
     2641 tccctgtttg cgggccgccg cttccagttg cgccatcgta attaattccc gttttaggtg
     2701 ttgcgttcgg accttgccgt cctcgatcaa aatatcggct tttccttcga tcaattgatc
     2761 cagggcgcga tggttgtata aaaatcgcac cacgaggtag ttaaccagca gcagggacgc
     2821 agcaccgata atcccgcctg ttaccgtatt gtcgtcgccg atgatggcgt tctgaacggt
     2881 gttggacagc gtcagcagga cgaccaggtc aaaaggattg agctgaacca gttcccgttt
     2941 tccggaaagc cgcaagctga tgaccagaaa cccgtacacc acaatgggcc gaagaatttt
     3001 ttctgcaatt ggcagcgcta gtacaaacat gtctttccac atatcgtgca tcgcgttcat
     3061 tcctcctgca cttgggggcc agagtctttg tgagactgct tgcgttcgac ttcgtaaggc
     3121 gagcgtagca cggcctttcg accgggcaaa ccaaagtact gtccgtcggg acagtcatga
     3181 tttcgcaaca gcgtaacttt ccgccgtggc atcgggccgg ccgcgcttat attcttactc
     3241 ccagttcggg ctgacttccg tcggcgtgtc cggttagcgc accagggccc ttgggcggta
     3301 gcacggcggt tctacagttt tatagccaga ccaaattcat catcgcgttc tttgagtcgt
     3361 ctttcgttta tgtcaaaaag ttggttgctc gcgcccgacg ctgcatgtac ggagtttcaa
     3421 gcccgcgctc tcctcgccgc tacgagagag ctgctggcca tcgtgtctgc ctccggccaa
     3481 tttctattcg taaatgacaa tttctcgcgt gtactcggat actcgccgca ggaactggtc
     3541 ggaaagtcgt tcctctctct tcactcggcc gccgacgcag cgctgattca gcaaaaattc
     3601 tcgtgcgccg tctccgcaag gaacgacgat actgtctcct gccg
//
LOCUS       DASTPM010000759         3820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_603969, whole genome shotgun sequence.
ACCESSION   DASTPM010000759 DASTPM010000000
VERSION     DASTPM010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603969"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /gene="lysS"
                     /locus_tag="VFR42_13415"
     CDS             <1..1548
                     /gene="lysS"
                     /locus_tag="VFR42_13415"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU0370210.1"
                     /translation="RDQFEQRQRKLEQIRQAGHNAYPHEFRVTATAGELAEKYSGSTT
                     ADLEANKVDARIAGRIVSYRLMGKAGFAHLQGGGGRLQIYVRKDVVGEKGFELFHLLD
                     LGDQIGVRGHLFRTKAGELSIWVDELTLLAKALLPLPEKWHGLTDRELRYRQRYLDLI
                     VNEKSRQVFLTRAKIVQELRRFFDARDYVEVETPMMHAIAGGAAARPFVTHHNTLDID
                     LYLRIAPELYLKRLVVGGLDRVYEINRNFRNEGISTQHNPEFTMLEFYEAYSNYQDLM
                     KMNEELLAELAQKIAGSTIVKYGEMDLDFGKIERLSMREAICKYWPEAARNAPTAREL
                     AAPGGAKSATERYNVWAKSKGAKYAACKGTLSDGEWTGLLFETIAEDKLQQPTILYDF
                     PTELSPLSKQKPDDASLTERFEIYIAGMEIANGFSELNDPAEQERRFLAQMAHGGDEV
                     PKQLDVDYVRALAHGLPPTAGEGIGIDRLTMLLTDSHSIRDVILFPQLRPENAARAGE
                     ESEGHGG"
     gene            1563..2789
                     /locus_tag="VFR42_13420"
     CDS             1563..2789
                     /locus_tag="VFR42_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0370211.1"
                     /translation="MAFAWFVAKRYLRSPHRPAVLRLVTGFSVLGVTAGVATLVVALA
                     MNTGFRETLQDRLLGVTAHISLTKPGSGGIHNYEALATKLGKIPGVRSATPAVYQTVL
                     MTFGGESRGVVTKGIDPVREEASDEALRKLAAGKLDFSPDADGIEALLVGKQLADEWK
                     VAPGDYVTLMSPQGRLTPFGLLPRTRKFRVAGIFDSGFYDYDANWCFLKLDAAQSLVG
                     APDVVNVLEFRLENPEAAPAVVREIEKEAGTGFAAATWMEEQRALFRALRLEKLVTTI
                     FIGLITLVAGLNILVVLTMTVTDRAKDIAVLRSMGAKSSQLRSIFLWQGFAIGAAGTI
                     LGLVLGYTFAWVAGSYRLVPLDPQVYAVPFVPFHPKMSDGVWIATVAMGISLLATLVP
                     ARAAARLLPVEILRFE"
     gene            3116..>3820
                     /locus_tag="VFR42_13425"
     CDS             3116..>3820
                     /locus_tag="VFR42_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0370212.1"
                     /translation="MSQARDNSGSEAGSPVLVAEAGRKVVLEARDISKSYGEGERSLN
                     VLQQVNLALYEGEMAAIVAPSGAGKSTLLHLLGALDTPTSGAIYFDLKHIETNDEAAL
                     SSFRNCAVGFLWQRHQLLPDFTAAENVAMPLLLRGESISKGLEKAGKWLGEVGLAERA
                     GRLAGELSGGEQQRVAIARALVTRPRVLLADEPTGDLDERNAWAVFELLERLHRAHGL
                     TSLIATHNLALAERCDR"
BASE COUNT          804 a          938 c         1292 g          786 t
ORIGIN      
        1 cgggaccagt ttgagcagag gcagcggaag ctggagcaaa tccggcaagc gggacacaac
       61 gcctacccgc atgaatttcg ggtgacggcc acggcgggag agctggcgga gaagtacagc
      121 gggtcgacaa ccgccgatct cgaagcaaac aaggtagacg cacggatagc tgggcgaatc
      181 gtttcctacc ggctcatggg gaaggctggg tttgcgcacc tgcagggcgg cggcgggcgc
      241 ttgcagattt atgtgcgcaa ggatgtggtg ggggaaaaag ggttcgagct gttccacctg
      301 ctcgacttag gggaccagat cggggtgcgc ggtcatctgt tccgcaccaa agctggcgag
      361 ctttctatat gggtggatga gttgaccctg ctcgcgaagg ccttgcttcc gttgccggag
      421 aagtggcacg ggctgaccga tcgagaactc cgctatcgac agcggtacct ggatctgatc
      481 gtcaacgaaa aatcgcggca agtttttttg acgcgagcga agatcgtgca ggaattgcgc
      541 agattttttg acgcgcggga ttatgtggaa gtggaaacgc cgatgatgca cgcgattgcc
      601 ggcggcgcgg cggcgcgccc tttcgtaacg catcacaata cgctggacat tgatttgtac
      661 ttgcggatcg caccagagct ctatctcaag cggctggtgg tgggcggact agaccgcgtg
      721 tacgaaatca accggaattt ccgcaacgag ggaatttcga cgcagcacaa tccggaattt
      781 acgatgctgg aattttatga ggcgtacagc aactatcagg atttaatgaa gatgaatgaa
      841 gagttgctgg cggaactggc ccagaaaatc gccggttcta cgatcgtgaa gtacggtgaa
      901 atggatctgg actttggaaa aatcgagagg ctgtcgatgc gcgaagcaat ttgcaagtac
      961 tggccagaag ccgcgcgtaa cgcgccaacg gcgcgcgaac ttgcagcgcc tggtggagcg
     1021 aaatcggcga cggaacgtta caacgtctgg gccaagagca agggggcgaa gtacgcggct
     1081 tgcaaaggca cgctgagtga cggcgaatgg acaggactgt tgttcgagac catagcggaa
     1141 gacaagctgc agcagccgac gattctctac gatttcccga ccgagctttc gccgctttcc
     1201 aagcaaaaac cggatgatgc gtcgctgacg gagcgattcg agatctatat tgcgggcatg
     1261 gagattgcca acgggttttc ggagttgaat gatccggccg agcaggagcg gcggtttctg
     1321 gcgcagatgg cacacggcgg agatgaggtt cccaagcagc tggatgtcga ttacgtgcgc
     1381 gcgctggcgc atggtttgcc gccaacagcg ggtgagggaa tcgggatcga ccggctgacg
     1441 atgctgttga cggattcgca ttcgattcgc gacgtgattc tctttccaca gcttcgcccg
     1501 gaaaatgcag cacgagccgg cgaagaatcc gaaggacacg gcgggtagcc gatgttttcc
     1561 tgatggcctt cgcatggttt gttgcaaagc gctatctgcg gtcgccgcac cggcccgccg
     1621 tgttgcgcct ggtgaccggc ttcagcgtac tgggtgtgac agccggagta gcgacgttag
     1681 tggtggcgtt agcgatgaac accggttttc gcgagacgct gcaggatcgc ctgctgggtg
     1741 tgaccgcgca catttccctg accaaacctg gttccggcgg aatccacaat tacgaagctc
     1801 tggcgacaaa gctgggaaag atacctggcg tgcgaagcgc gactcccgcc gtttaccaga
     1861 ccgttctgat gacgttcggc ggagaatcac gcggcgtggt gacgaagggg attgacccgg
     1921 ttcgagaaga ggcttccgac gaagccttgc gaaagctggc cgcgggcaag ctggattttt
     1981 caccggatgc agatgggatt gaggcgttgc tggtcggcaa acagttggcc gatgaatgga
     2041 aggtcgcgcc gggagactac gtcacgctga tgagtccaca agggcggttg actccatttg
     2101 ggttgttgcc gcgaacgcga aaatttcgcg tggcgggaat ttttgattca ggattttacg
     2161 attacgatgc caactggtgt tttctgaaac tagatgcggc gcaatcgttg gtcggcgcgc
     2221 cggatgtagt gaacgtcctg gagttccgcc tggaaaatcc cgaggcggcg ccagcggttg
     2281 tgcgggaaat agagaaggaa gcgggaacgg gctttgcagc agcgacatgg atggaagagc
     2341 agcgggcgct gttccgcgcg ctgcggctgg agaaactggt gacaacaatt tttatcgggc
     2401 tgatcacgct ggtcgcgggc ttgaacattc tggtggtgct gacgatgacg gtgacggatc
     2461 gcgcgaaaga catcgcggtg ctgcggtcca tgggcgccaa gtccagccag ctgcgcagca
     2521 tcttcctgtg gcaagggttt gcgattggcg ctgccggaac catcctggga ttggtgctcg
     2581 gctatacctt cgcttgggta gcgggcagct atcggctggt tccgctggat cctcaggtgt
     2641 acgccgtacc ttttgtgccg ttccacccca agatgagcga cggggtgtgg attgcgacgg
     2701 tggcgatggg aatatccctt cttgcgaccc tggttcctgc aagggcggcg gcgaggttgc
     2761 tgcctgtgga aatccttcgt tttgaatgaa ttagcccgaa acaaggctgg gtgcgtttgg
     2821 ctatccgctt gcacctatga gttctagcga gtttgcgtgc gggttcacct cggcgttgag
     2881 cctagcgggc cgacaaccga tagcctttgc aagtgactga tttctgaggc gttgcgagct
     2941 tatcgtggcg ggacttttct gagggataaa acgaaggcga accgggttcc acttagggaa
     3001 cggtttggcc ggcacggtgt cacaacgagg agacgccatt tttcgagcgc cggacgcatc
     3061 taaactctta tcgggcgcga gacggagctg cacgctgcgg agaatttcag acgcgatgag
     3121 ccaggcaagg gacaattcgg gttcggaagc gggttcacct gtgctggtgg cggaggccgg
     3181 ccgcaaagta gtcctcgaag cgcgcgacat cagtaagagc tacggagaag gcgagcgcag
     3241 cttaaacgtt ttgcaacagg tgaatcttgc gttgtacgaa ggcgagatgg cggcgattgt
     3301 agcgccgtct ggagctggca aaagcacttt gctgcatcta ctgggtgcgc tagacacgcc
     3361 aacgagcggt gctatatact tcgatttgaa acacattgaa acaaatgatg aagcggcgct
     3421 ttcgagtttt cgtaattgcg ccgtagggtt tctgtggcag aggcatcagt tgctgccgga
     3481 ttttacggcg gcggaaaatg tagcgatgcc gctgctgctg cggggcgagt ctatatccaa
     3541 aggcctggaa aaggccggga aatggctcgg ggaggttggc ttggcggagc gtgccgggcg
     3601 cctcgcggga gagctttccg gaggggaaca gcagcgcgtg gcgattgccc gggccctcgt
     3661 caccaggccg cgcgtgctgc tggccgatga acctaccgga gacttggacg agcggaatgc
     3721 ttgggccgta ttcgagttgc tggagcgatt gcatagggca cacgggttga cgtcgttaat
     3781 cgcgacgcac aatctggcgc tggccgaacg gtgcgaccgg
//
LOCUS       DASTPM010000760         8898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_344729, whole genome shotgun sequence.
ACCESSION   DASTPM010000760 DASTPM010000000
VERSION     DASTPM010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8898
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344729"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFR42_13430"
     CDS             complement(<1..956)
                     /locus_tag="VFR42_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU0370213.1"
                     /translation="MRVAVLHQTGQSPRLETFSDPVAGENEVLLTVLAASLKPIDKQL
                     AAGTHFASPREFPVICGSDGVGRLADGSRVFFGGPRRPYGTFAERTVVRCAQCFPIPE
                     TLDDATAAAIPNPGVSAWLSLSHRARLVAGESVLILGATGVTGQLAVQIAKILGASRV
                     VAAGRNPESLAALPARGADVTISLNQRGESLMEAFRRQNSEKRFDVIIDYVWGPPTEA
                     LLAAITAREFGASNSETRLVQVGESAAPTIQLPAAALRSVALTLMGTAGIPPMDVLSG
                     ALNQVFVHAAKGDLRIETEQLPLSELEAVWNRPSSRRIVFCP"
     gene            complement(1048..1452)
                     /locus_tag="VFR42_13435"
     CDS             complement(1048..1452)
                     /locus_tag="VFR42_13435"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0370214.1"
                     /translation="MKRLLTFVLILVTLFGTALVATRTVFAAPQHRGSSTLSGVVLGP
                     NDKPVARAVITYQSSAGSAPHVVRTDSHGRFSVAKLPADNYDLRASANGIFSEWQKNV
                     SIRSGETHSVTLRLIYAKQMPKPGTITAGTVK"
     gene            1791..2714
                     /locus_tag="VFR42_13440"
     CDS             1791..2714
                     /locus_tag="VFR42_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0370215.1"
                     /translation="MPTKRRILLSLMTEENDFQLEQAASARRAAEALPVDLQILFAGN
                     DTITQSTQILRAIQGDPALRPDAIIFEPAGGTALPQVARAACSAGIGWCVLINIPDYL
                     SELRRGARAPVFVVSTDQEEIGRIQAKQLAALLPRGGSALYIQGPSEHFAAKQRTLGL
                     QSGLPQNIRISGLRGNWTEEGAARSVSSWLKLTAANKIRVDLVAAQNDLMALAARKVF
                     QGVSDLMERDRWMSVPFVGVDGLPKTGQSWVRDRLLSATVIMPPSAGQALTLMMGALG
                     KPGQSVPEKIWLPPQSFPSLEELRPSQVPPD"
     gene            complement(2766..4916)
                     /locus_tag="VFR42_13445"
     CDS             complement(2766..4916)
                     /locus_tag="VFR42_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0370216.1"
                     /translation="MDAGTLKRHGIPVKLQEQPFRILTFLVERPGNIISREELCARLW
                     PEGTFVEFDGSLNTALMKLRAVLNDDPENPRFVETVPKKGYRFIAPVHFLEPMPATAI
                     AEAEKEPAAVPQSAAEIPLSTDIRISTFPADLPKSQRRALVALIALAVATVLAAAVVW
                     RYWPVSDPQVLKVTQLTHTGRLAPGSQIVSDGARLYFVSRENGQWTLMTTSVHGGSVE
                     KLPSPFENSMLFDLSPDRTQLLIGPFAPEGDQIPLWIWPAHGGVPHRLGDVTASDAAW
                     SPIGDRIAFTRGDRLLTVRRDGSAASEIHKFSGRPRSPVWSADGTKIRFTLSDSEKST
                     DDIWEIGADGKGLRRVPVNSATGSDLHETTGAWTSDERYFLFTAGADSNLSEVIDSSS
                     NIWAAHERAGIFSRKRPPVELTHGPMAFRNVTGTGDSNRFFALGTHPEYQLIRMDPRS
                     GRTETILPDAGATDVDVTPDGEWLVYSLRENGALWKSRRSGKERIELTASSPGALAPQ
                     WSPDGNEVLFTEFFLTRRPQLYLVPSNGGIPHPLLSGAASGSSRSGDWSSDGKLVLFD
                     YFEGDDSDLRIMERDSGKVETLLDSQGLVGPRWSPDGKYIAAINSRTHDILLYTIATK
                     KWSVLAEAINAQGMRWSADSNYVYFQEMADAEQSVYRVRVGRALAEKVVGFKDYIGSM
                     ASQCHFTGVAPDGSVYATVDRGGTDIYALDLKLP"
     gene            5398..6015
                     /locus_tag="VFR42_13450"
     CDS             5398..6015
                     /locus_tag="VFR42_13450"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HEU0370217.1"
                     /translation="MVLPRQSRTNQTRRLALCAALSALIVVCTMGGHAYTSLPQKSTV
                     ENLDGKPLDPFAASANRVVALLFVRTDCPISNRYAPLLQKMSEKFRGRADFWLVYPDR
                     AETASQIKAHLQDYHYSIAAVRDTDHALVRRAQATITPEAAVFDSSGRLLYHGRIDNW
                     YEDFGRSRPAATTHELDDAISSAIAGKPIHPDHANAVGCYISDLT"
     gene            6054..7850
                     /locus_tag="VFR42_13455"
     CDS             6054..7850
                     /locus_tag="VFR42_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0370218.1"
                     /translation="MSSLIFIVATLYASAHSSQTAEPVVAENSALTTFTRDIAPIVFR
                     TCAPCHHAGEAGPFSLVTYGDAKSHARQIVDVTSKRLMPPWLPSADSLRFEEDAHLSD
                     HDIALFQKWLDDGLREGDPADLPPVPKFSSGWQLGQPDLILHAAAPYTIPSSGSDVYW
                     NFIFRSPLNSSRFIKAIEIHPGDKRLVHHANLLVDRMQSARRQEKSPGSGFASMELQI
                     ESESFDPDGHFLFWKPGSPPIVEPPGLALRLDPENDLVLNTHLQPSGKPESVQPTIGI
                     YFTNEPATRFPVLLQIENDRALNIPAGDNNFVVSDAFTLPENVQLLALYPHAHYLCRD
                     MLATARLPDGTEKNLIHIPRWDLNWQAVFRLAQPEPFPRGTTIVMSYRYDNSADNIAN
                     PNNPPQRVTAGNRAVDEMAHLWLQVLPETQPGQQRDPRMVLQESLARHHIENNPADFE
                     AHYNLAAMLQARGASDEAVLHYQSALKLRPGDAVVENALGAALLSSGKIPGAIQHLSA
                     AAASRPDYFDAHYNLGIGLASAGEFARAAQEFAAAAKLKPEDSGAEANLGAAFAELGQ
                     NEQAIAHFQRALQLDPKNQLARENLEALQKAP"
     gene            7986..>8898
                     /locus_tag="VFR42_13460"
     CDS             7986..>8898
                     /locus_tag="VFR42_13460"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEU0370219.1"
                     /translation="MRRIVSLVVLSIAICSTAFALSRTPRPASPPPDTTLLHGSSRQP
                     PVNNWTVVRLSGTPKQIGYQHGYLLAPEIADLQKVFLLELTHDNGKDWNFFRDAAKNV
                     WWSHIDQEYRDEMQGIADGVTARGVQLDVWDIVVINGAMEWSYYVGQYDKDHKIASPP
                     TVTAPDHCSAFVATGSYTKDRNIVIAHNNWTGYMDGSRWTIAFDIKPANGYRFVMDGL
                     PGVIHSADDFGINSAGIVITETTITGFSGYDFNGIPEFVRARRAMQYSASIDDVAKIF
                     KEGNNGGYANDWLIADTRKNEIASLELG"
BASE COUNT         1896 a         2892 c         2332 g         1778 t
ORIGIN      
        1 cacgggcaaa agacgatacg ccgagatgat ggacggttcc aaacagcttc cagctcggac
       61 aagggaagct gttcggtttc gatgcgaagg tcgccctttg cggcatgaac gaagacttga
      121 ttcaacgcac cggaaaggac atccatggga ggaatcccgg cggtccccat gagggttagc
      181 gcgacgctgc gcagggcggc agcaggcagt tgaatggtgg gcgcggcaga ctcgccgact
      241 tgcaccaggc gcgtctcaga attgctcgcg ccgaattcgc gcgctgtgat ggcggcgagt
      301 aatgcttcgg tgggcgggcc ccagacgtaa tcaatgatca cgtcgaagcg tttttccgaa
      361 ttttgccggc gaaaagcctc catcagggat tcgccgcgct gattcaagga aatcgtgacg
      421 tcggcaccgc gtgcggggag cgctgccaga ctttccggat tacggccggc tgcgaccacg
      481 cgcgatgccc ctaaaatttt tgcgatttgc acggcaagtt gtccagtgac accagtcgct
      541 ccgaggatca gaacgctctc acccgccaca agcctggcgc gatggctaag cgagagccag
      601 gccgaaacgc cggggttagg aatggccgcc gcggtagcgt cgtcgagagt ttcaggaatt
      661 ggaaagcact gcgcgcagcg cactacggta cgctcggcga acgtgccgta aggccggcgc
      721 ggacctccaa agaaaacgcg cgaaccatcc gccaggcgac cgacgccatc ggagccgcaa
      781 attacgggga actcgcgcgg gctggcgaag tgagttcctg cggcgagctg cttgtcgatt
      841 ggctttaaag aagcagccag caccgtgagc agaacttcat tttccccggc aaccggatct
      901 gaaaaggtct ccagacgagg agattggccg gtttggtgga gcacagcaac tcgcatggcg
      961 aaatgatggt gacaaaagcg agtcgcgctg gcaatcacgt tcaacgggaa atcagaagtc
     1021 gcggcgtgga acttcgcgaa ggcgaatcta tttcacggta cctgcggtaa tcgtgccggg
     1081 cttgggcatt tgtttggcgt agatcaaacg tagcgtgacg ctgtgtgttt cgccggaccg
     1141 aatgctgacg tttttctgcc attccgaaaa aataccgttg gcggatgcgc gaaggtcgta
     1201 gttgtcggca ggaagtttcg ctacgctaaa acggccgtgc gagtctgtgc gcaccacgtg
     1261 aggcgcgctg cccgccgaag attggtacgt aatcacagcg cgcgcgacgg gcttgtcgtt
     1321 gggaccgagc accacgccgc tgagcgtgct gctgcctctg tgctgtggtg cagcgaaaac
     1381 ggtgcgcgta gcgaccagcg ccgtgccgaa caacgtgacc aaaataagca cgaaagtgag
     1441 cagtcgtttc ataaggtctc tcggccggga gcgccctccg gcgatcagga aatcagttta
     1501 ggagatgggg agtcatcgcg gcggagaatc agaaaattgt gcctcaggta aaacctgtca
     1561 ccaagaacag gtgcagagta cagctgtgcc ccgaggagag cgccacgaat cgtcagacga
     1621 tccgcccaat tcggcagctt gcctttggag tttagaaaat tcagtacaca gtgaaccgac
     1681 gggtcacatt tttgaaacga ccgcaaatct ccgtccaacc cgcggcgagt acaggtgtac
     1741 tctaagccga ccgaacgtga attcaattca tcctgccctg gagagttgcg ttgccaacca
     1801 agcgccgcat tttgctgtct ttgatgacgg aggagaacga ctttcagttg gaacaggcgg
     1861 cctctgcccg gcgagctgcc gaagccttgc cggtggatct gcaaatcttg ttcgccggca
     1921 acgacaccat tacccaaagc acacaaattc tgcgcgccat tcagggcgat cccgctttgc
     1981 ggcccgatgc catcattttt gagccggcgg gcggaacggc acttccgcag gtggcgcgcg
     2041 cggcttgcag tgcaggcatt ggctggtgcg tgctgattaa catccccgac tatctttcgg
     2101 aattgcggcg cggcgcgcgg gcgcccgtgt ttgttgtaag cacggaccag gaagaaatcg
     2161 gacgcatcca ggcgaagcaa cttgccgctc tgttaccgcg cggcggcagt gcactgtata
     2221 tccagggtcc gtccgaacat tttgccgcca agcagcgcac gctgggtttg caatcaggac
     2281 ttccgcaaaa cattcgcatc agcggcctgc gtggcaactg gacagaagaa ggcgcggccc
     2341 gcagcgtcag ctcctggttg aagctgaccg cagcaaataa gattcgtgta gacctggtcg
     2401 ccgcacaaaa tgacctgatg gctctggcgg ctcgcaaagt gtttcaaggc gtgagcgatc
     2461 tcatggagcg cgaccgatgg atgagcgtgc cctttgttgg cgtggacggt ttaccaaaga
     2521 ccggtcagtc ttgggtgcgc gaccgcttgt tgagcgccac cgtgattatg ccgccgtccg
     2581 ccgggcaggc cctgactctg atgatgggag cgctcggcaa gccgggccag tccgttccag
     2641 aaaagatttg gcttccgccg caatcgtttc catctctgga agagctgcgc ccgtcgcagg
     2701 tcccgcccga ctgattgaag actccctcgg tctgaagcga gaaatattcg ccagccgaac
     2761 ggatgctagg gcagcttaag gtccaacgcg taaatatccg taccgccgcg atccaccgtg
     2821 gcatacaccg agccatcggg cgccacgccc gtgaagtggc attgcgaagc catcgaaccg
     2881 atataatcct tgaaaccgac aactttctcc gctaacgcgc ggcccactcg cacgcggtag
     2941 actgattgct ccgcgtcagc catttcctgg aaataaacat agttgctgtc ggctgaccag
     3001 cgcatgccct gcgcgttgat ggcctccgcc agcaccgacc acttcttggt agcaatcgta
     3061 tagagcaata tgtcatgcgt tctgctgttg atggccgcaa tatattttcc gtccggcgac
     3121 caccgcggtc caaccagtcc ttgcgaatct agtagtgttt ccacttttcc ggaatcgcgc
     3181 tccatgattc gcaaatccga atcatcgcct tcaaaataat caaaaaggac cagcttgccg
     3241 tcggacgacc agtcgccaga ccgcgacgat cccgatgccg cgccactcaa caatggatgg
     3301 ggaatgccgc cgttgctcgg gaccagataa agttgcggtc gcctggtcaa aaaaaactct
     3361 gtgaacagca cttcgttgcc gtccggcgac cactgcggcg ccaacgcgcc cggagaagaa
     3421 gccgtaagct cgatgcgctc cttcccgctg cgccggcttt tccagagtgc gccgttttcg
     3481 cgcagcgaat acaccagcca ctcgccatcg ggtgtcacgt ccacatcggt cgctccggcg
     3541 tccggcagaa tggtctcggt ccggccggat cgcggatcca tgcgaatcaa ctggtattca
     3601 ggatgggtgc cgagcgcgaa gaatcgattg gaatctcccg tacccgtcac gttgcggaat
     3661 gccatggggc cgtgcgtcag ctcgacagga gggcgtttgc gggaaaaaat tcccgccctt
     3721 tcgtgagcag cccagatatt cgacgacgag tcaatcactt cgctgagatt cgaatcggcc
     3781 ccggcggtga acagaaaata tcgctcgtcg ctcgtccagg caccggtggt ttcgtgcaag
     3841 tcgctccccg tcgcgctgtt gaccggcacc cgccgcagtc ctttcccgtc cgcgccaatt
     3901 tcccaaatat catcggtcga tttctctgaa tccgacagcg tgaagcgaat cttggttccg
     3961 tcagcggacc acaccggtga acggggtcgc cctgagaatt tgtggatctc gctcgcggcg
     4021 gagccgtcgc gccgcaccgt caacaagcgg tcgccccgtg taaatgcaat ccggtcgccg
     4081 atgggagacc aagccgcgtc gctcgctgtt acatcgccca gtcgatgagg tacgccgcca
     4141 tgcgccggcc aaatccaaag cggtatttga tcgccctccg gtgcaaatgg accgatgagc
     4201 aattgcgtac ggtccggcga gaggtcaaac agcatcgaat tctcgaaagg tgagggcagc
     4261 ttctccacgc tgccgccatg tacggacgtg gtcatcaacg tccactgtcc gttttcgcgc
     4321 gaaacaaagt acagtcgcgc cccgtcggaa acgatctggc tgcccggcgc aagtcgcccg
     4381 gtatgcgtca gttgcgtgac ctttagaact tgcggatccg ataccggcca gtagcgccac
     4441 accactgccg cggctaaaac ggtcgccact gccagcgcaa taagtgcgac gagcgcccgc
     4501 cgctgcgatt tcggcaaatc cgcggggaac gttgagatcc ggatatcggt actgagcgga
     4561 atctcagccg cactttgtgg cactgccgcg ggttccttct ccgcctctgc gatagccgtc
     4621 gccggcatag gctcgaggaa atgcactggc gcaataaacc ggtacccctt cttcggcacc
     4681 gtctccacaa atcgcggatt ttcgggatcg tcattcagta cggcgcgcaa cttcatcaac
     4741 gctgtgttca ggctgccatc aaactctacg aatgtgccct ccggccagag tcgggcacac
     4801 aactcctcgc ggctaataat gttgcctgga cgttccacca gaaaggtcag gattcgaaac
     4861 ggctgctctt gcagcttcac aggaatgccg tgacgtttca acgtcccagc atccaaatcg
     4921 gcctcatata ggccgaagcg gcatatccga tgttcagaaa tttgaggagt catcgccagt
     4981 attgaatcct catcgttttt tcgagttggt tgatatcttt ccccaaactc ttagttatcc
     5041 atagtttacc agattattcg ggcttgataa acagttcgtt tgacccaata caagccttcc
     5101 ctcatcctgc atccacggtg agcagacttg agcccgaaac tccaggtctt cgggaacaac
     5161 gctgcttagg agaccggagg agaatccctg ccagagtgga ttcttctcta ggtctccatt
     5221 tttatttcta gcgcctcccc tctgttagtt atctaccggc cttcaccagg cattccgctc
     5281 atcccagctt tcctcccgta ttgccgtaat tcgcttttca atgtttgaat aaccatagcc
     5341 tcggccacag aaatcgccga agagtgtaag ataaatcgcc ggaacggaat tcccagcatg
     5401 gtcctaccac gccaatcgcg caccaaccag actcgccgcc tggccctgtg tgccgcacta
     5461 tccgccttga tcgtggtctg cacaatgggc ggacacgcgt acaccagcct gcctcaaaaa
     5521 tccacagtgg aaaatttgga cggcaaaccc ctcgacccct tcgccgcctc cgcaaatcgc
     5581 gtcgtcgccc tgctctttgt gcgaaccgac tgccccatct ccaaccgcta cgcgcccttg
     5641 ctgcaaaaaa tgagcgaaaa gtttcgcggc cgagccgact tctggctggt ctatccagac
     5701 cgcgccgaga ccgcctcgca aatcaaagcg caccttcagg actaccatta ttccatcgcc
     5761 gcggtccgcg acaccgatca cgctctcgtc cgccgcgctc aggccaccat aactcccgaa
     5821 gccgccgtct tcgattcatc cggtcgcttg ctttatcacg gccgcatcga caattggtac
     5881 gaggactttg gccgctcgcg ccccgccgcc accactcacg aactcgacga cgccatctcc
     5941 tccgccatcg ctggcaagcc tatccatccc gaccacgcca acgccgtcgg ctgctatatt
     6001 tccgacctca catgaagcgc accgctgcac gccggccatt catcgtttct tctatgtctt
     6061 ccctcatctt catcgtcgcc actctctatg cttcggcgca ttcgtcacaa accgcggaac
     6121 ccgtggtggc tgaaaattcc gcgctcacca cattcacgcg cgatattgcc cccatcgtct
     6181 tccgtacctg cgcgccctgc caccatgcgg gcgaagccgg ccctttttcc ttggttacct
     6241 acggcgacgc gaaatcccac gcccgccaga tcgtcgacgt caccagcaaa cgcctcatgc
     6301 ctccgtggct cccttccgcc gacagcttgc gctttgaaga agacgcgcac ctctcagatc
     6361 acgacatcgc cctgtttcaa aagtggctag acgacggcct gcgcgaaggc gatcccgccg
     6421 atctccctcc cgtcccgaaa ttctcctccg gatggcaact cggccaaccc gacctcatct
     6481 tgcacgccgc agcgccctac accattccct ccagcggtag cgacgtctac tggaatttca
     6541 ttttccgctc ccctctgaac tcctcccgct ttatcaaagc catcgaaatc catcccggcg
     6601 acaaacgcct cgtgcatcac gccaaccttc tggtcgaccg catgcaatcc gctcgccgcc
     6661 aggaaaaatc tcccggcagc ggctttgcca gcatggaact gcaaatcgaa tccgaatcct
     6721 tcgatcccga tggacacttt cttttctgga agccgggcag cccgccgatc gtcgagccgc
     6781 ctggcctcgc cctgcgtctc gatcccgaaa acgatctggt gctcaacacc cacttgcagc
     6841 cgtccggcaa accggaatcg gttcaaccca ccatcggtat ttacttcact aacgagccag
     6901 ccactcgttt tccggtcttg ctgcaaattg aaaacgatcg cgcgctcaac atccccgccg
     6961 gcgacaacaa tttcgtggtt agcgacgcat tcactcttcc cgaaaacgtc caactcttgg
     7021 cgctctatcc ccacgcccac tatctctgcc gcgacatgct cgccaccgcg cgccttccgg
     7081 acggcaccga aaaaaatctt atccacatcc cccgctggga tctcaactgg caagccgttt
     7141 tccgcttggc ccagcccgaa ccattcccgc gcggcaccac cattgtcatg agctaccgct
     7201 acgacaattc agccgacaac atcgccaatc caaacaaccc gccgcagcgt gtaaccgcgg
     7261 gcaatcgggc cgtcgacgaa atggctcatc tctggctgca agtgctgcct gaaacgcaac
     7321 ctggccagca gcgcgatccg cgtatggttc tgcaggaatc gctcgcccgc caccacattg
     7381 aaaacaatcc cgccgatttt gaagcccact acaatctcgc ggccatgctg caagcgcgcg
     7441 gtgccagcga cgaagcggtg ctgcactacc aatccgctct caaactccgg cccggcgacg
     7501 ccgtcgtcga gaacgcactc ggcgctgccc tgttgtcttc gggaaaaatt cccggcgcca
     7561 tccagcatct ttcggccgcg gctgcttccc gccccgatta tttcgatgct cactacaatc
     7621 tcggcatcgg gctcgcttcc gccggagaat ttgcgcgcgc cgcccaggaa tttgccgcag
     7681 cagccaaact aaaacccgaa gactccggcg ccgaagccaa ccttggcgcc gccttcgccg
     7741 aactcggcca gaacgaacaa gccatcgccc atttccaacg cgctctccaa ctcgacccga
     7801 aaaaccagct ggcccgcgaa aatctcgagg ccctccaaaa agcgccatag tcaaactcga
     7861 atccttagca aggtcatcct ttgcaactcc aataggagga aactcccaag ccacgcgccc
     7921 aagccacgcg cccttgtcaa accctcccgc gcactgtact ttttctactc cagcctggag
     7981 gttccatgcg ccgcatcgtg tccctggtcg tcctgagtat cgcgatttgc tccaccgcct
     8041 ttgctctctc gcgaactccc cgccccgctt cacccccgcc cgatacaaca ctcctccatg
     8101 gctcctcccg ccagcccccc gtcaacaatt ggaccgtcgt ccgtctttcc ggcactccca
     8161 aacagatcgg ttatcagcac ggctacctgc tcgcgcccga aatcgccgac ctgcaaaagg
     8221 tttttcttct cgaactcact cacgacaacg gcaaggactg gaatttcttc cgcgacgccg
     8281 ccaaaaacgt ctggtggtca cacatcgatc aggaatatcg cgacgaaatg cagggcatcg
     8341 ccgatggcgt caccgcgcgc ggcgttcagc tcgacgtctg ggacatcgtc gtcatcaatg
     8401 gcgccatgga gtggagctac tatgtcggtc agtacgacaa ggatcacaaa attgcttcgc
     8461 cccccactgt cactgctccc gaccactgca gcgcctttgt cgccaccggc agctacacca
     8521 aagaccgcaa catcgtcatc gctcacaata attggaccgg ctacatggac ggctcgcgct
     8581 ggaccatcgc cttcgacatc aagcccgcca acggctaccg cttcgtcatg gacggcctgc
     8641 ccggcgtcat ccacagtgcc gacgatttcg gtatcaactc tgccggcatc gtcatcacgg
     8701 aaactaccat caccggattt tccggttacg acttcaacgg cattccggaa tttgtccgtg
     8761 cccgcagggc aatgcagtac tccgcatcga ttgatgacgt ggcgaaaatt ttcaaggaag
     8821 gcaacaacgg cggttacgcc aacgattggc tgattgcgga cactcggaaa aacgaaatcg
     8881 ccagtctcga actgggcc
//
LOCUS       DASTPM010000761         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_748418, whole genome shotgun sequence.
ACCESSION   DASTPM010000761 DASTPM010000000
VERSION     DASTPM010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748418"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2178
                     /locus_tag="VFR42_13465"
     CDS             <1..2178
                     /locus_tag="VFR42_13465"
                     /inference="COORDINATES: protein motif:HMM:NF044398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEU0370220.1"
                     /translation="YTIDEKHRTATLTEEGVSKTERLMGLGNLYDPAHMEIIHHVYQA
                     LRAHTLYKRDVDYVNKDGEIIIVDEFTGRQMPGRRWSDGLHQAVEAKEGVKIERENQT
                     LATITFQNYFRMYKKLSGMTGTAETEAAEFEKIYKLDVVVVPTNRALIRIEHPDVVYR
                     TEKEKFEAVVNGILQEDNSLAHGIKHYHDRGQPVLVGTISIEKSETIADILKKSGVPH
                     EVLNAKQHERESRIVAQAGRKGAVTVATNMAGRGTDILLGGNPEFMTREHFLKNRLAM
                     PYAQAPAVIGADAENGHAPGAQAASNVSMVLFQHEGKIFQVPHDQWKPVYDDFANQCK
                     AEHDEVVGLGGLHILGTERHEARRIDNQLRGRAGRQGDPGSSRFFLSLEDDLMRIFGG
                     ERVKALMFRLGMTEGVPIESGLISRRIENAQKSVEAQNFDARKHLLEYDDVMNKQRET
                     IYAIRRSALEGKDQRDYVLGIAEDIARELVDTYCPRDQHPGEWNTTQLLAEIHSQFGV
                     DAKDAGIDPSALNHDQLSDAVAEAVIQKYSDKEKQLTPDLLRWLERRIILDVVDSQWK
                     DHLLSLDHLKEGIGLRGYGQKDPLVEFKKEAFILFEDMMARIDNETIRYLFNVQVQME
                     GAVPPDGAPQPRPTPPAAARPFAAAASAAARVADEPVQQLPAFAREMERKQQRQEKEM
                     QYQAGAAQAEPPKPVRAGAKVGRNDPCPCGSGKKYKKCHGVNA"
     gene            2279..3121
                     /locus_tag="VFR42_13470"
     CDS             2279..3121
                     /locus_tag="VFR42_13470"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU0370221.1"
                     /translation="MPPGSAPQQMPERQPIPGVKNLVAVASGKGGVGKTTVAVNLALA
                     LHKLGNKVGLLDADVYGPNVPIMLGTNQEPLATADRRIVPVEIAGLKMISMGLLNPGD
                     KPMIWRGPMLHSVITQFLRSAEWGQLDYLIIDLPPGTGDVQLTLIQTVAVSGAVVVTT
                     PSTVALADVRKAIEMFRQVNVEVLGVVENMSTFACPHCGKPVDIFGHGEGAKTARAYG
                     VPVLGEIEIDPRIRIGGDTGKPVATLGEDAAAAKSLYAVARAVSARLEELGSSTLGPQ
                     VQID"
BASE COUNT          836 a         1273 c          871 g          657 t
ORIGIN      
        1 tacaccatcg atgaaaagca ccgcaccgcc acgttgacgg aagaaggcgt cagcaagacc
       61 gaacgcctca tggggctcgg caacttatac gaccctgcgc acatggaaat catccatcac
      121 gtttatcagg cattgcgcgc gcacactctc tacaagcgcg acgtcgatta cgtcaacaaa
      181 gacggcgaaa tcatcatcgt ggacgaattc accgggcgcc agatgcccgg ccgtcgctgg
      241 tctgatggcc tccaccaagc cgtcgaagcc aaagaaggcg tcaagatcga gcgcgaaaac
      301 cagacccttg ccaccatcac cttccagaat tatttccgca tgtacaaaaa gctttccggc
      361 atgaccggca ccgcggaaac ggaagccgcc gagtttgaaa agatctacaa acttgatgtg
      421 gttgttgttc cgacgaaccg tgcgctcatc cgcatcgaac atcccgacgt cgtctaccgc
      481 accgaaaaag aaaaatttga agcagtcgtc aacggcatcc ttcaggaaga caactcactg
      541 gcccacggta tcaagcacta tcacgatcgc gggcagcccg tcctcgtagg caccatctcc
      601 atcgaaaaat cggaaactat tgccgacatc ctgaagaaat ccggcgtgcc gcacgaagtt
      661 ctaaacgcca agcagcacga acgcgaatcc cgcatcgtcg ctcaggccgg acgcaaaggc
      721 gccgtgaccg ttgccaccaa catggctggc cggggtaccg acattcttct cggcggcaat
      781 cctgaattca tgacccgcga gcatttcttg aaaaaccgcc tcgcgatgcc ctatgcccag
      841 gctcccgccg tcatcggcgc cgacgccgaa aatggccatg cccccggcgc acaagctgcc
      901 tccaacgtct ccatggtgct cttccagcac gaaggcaaga tttttcaagt cccgcatgac
      961 cagtggaaac ccgtctacga cgatttcgcc aatcagtgca aagccgaaca cgatgaagtc
     1021 gtcggcctcg gtggcctcca cattctcggc accgagcgtc acgaagcccg ccgcattgat
     1081 aaccagctcc gcggacgtgc cggccgccag ggcgaccccg gatcctcccg cttcttcctc
     1141 tccctcgaag acgatctcat gcgcatcttc ggtggcgaac gcgtcaaagc cctcatgttc
     1201 cgcctcggca tgaccgaagg cgtgcccatc gaatccggcc tcatctcccg ccgcatcgaa
     1261 aacgcgcaaa aatccgtcga agcacaaaac ttcgacgccc gcaaacacct cctcgaatac
     1321 gatgacgtga tgaacaagca gcgcgaaact atctacgcca tccggcgttc cgcgctcgaa
     1381 ggcaaagacc agcgcgacta cgttctcggc atcgccgaag acatcgcccg cgaactcgtc
     1441 gacacctact gcccccgcga ccagcatccc ggcgaatgga acactaccca gctgctcgcc
     1501 gaaattcaca gccagttcgg tgttgacgcc aaagacgcgg gcatcgatcc gtccgctctc
     1561 aatcacgacc aactctccga cgccgtcgcc gaagccgtca tccaaaaata ttccgacaag
     1621 gaaaagcagc tcactcccga tcttctccgc tggctcgaac gccgcatcat tctcgacgtc
     1681 gtcgattccc agtggaaaga ccacctgctc tctctcgatc acctgaaaga gggcattggc
     1741 ctgcgcggct acggccaaaa agatcccctg gttgaattca agaaagaagc cttcatcctt
     1801 tttgaagaca tgatggcgcg tatcgacaac gaaaccatcc gctatctctt caatgttcag
     1861 gtgcaaatgg aaggtgctgt gccacccgat ggcgctccgc agccccgacc cactccgccc
     1921 gccgcggctc gtccgtttgc cgctgcagcc agcgccgccg cacgtgtagc cgacgaaccc
     1981 gtccagcaac tccccgcttt cgcgcgcgaa atggaacgca agcaacagcg ccaggaaaaa
     2041 gaaatgcaat accaagccgg cgcagcccaa gccgaaccgc ccaaacctgt gcgcgcaggc
     2101 gccaaagtcg gccgcaatga cccgtgcccc tgcggctccg gcaaaaaata caaaaaatgc
     2161 cacggcgtca acgcctagtt cggccgcgta atgctcttga ttctgaattc ataattcgtc
     2221 attccgaacg ctgcgaggaa tctctcttcg gctgacgcta gaaagcgaac ttgaccaaat
     2281 gccaccagga tcagcgcccc agcagatgcc cgaacgccag cccatccccg gcgtaaagaa
     2341 tctcgtcgcc gtcgcctccg gcaaaggcgg cgtaggcaag accaccgtcg ccgtcaatct
     2401 cgcgctcgcg ctgcacaaac tcggcaataa agtcggcctc ctcgacgccg acgtctacgg
     2461 ccccaacgtc cccatcatgc tcggtaccaa ccaagagccg ctcgccaccg ccgaccgccg
     2521 catcgttccc gttgaaatcg ccggcctgaa aatgatctcc atgggcctgc tcaatcccgg
     2581 cgacaaaccc atgatctggc gcgggccgat gctccacagc gtcatcacgc aattcctccg
     2641 ctccgccgaa tggggccagc tcgattacct catcattgat cttccgcccg gcacgggcga
     2701 cgtccaactc acgctcattc agaccgtcgc ggtctccggc gccgtggtcg tcaccacacc
     2761 ttccacagtc gccctcgccg acgtgcgcaa agccatcgaa atgtttcgcc aggtcaatgt
     2821 cgaagtgctc ggcgtcgtcg aaaacatgtc caccttcgct tgcccccact gcggcaagcc
     2881 cgtcgacatc ttcggccacg gcgaaggcgc caaaaccgcc cgtgcttacg gcgttcccgt
     2941 cctcggcgaa atcgaaatcg atccccgcat ccgcatcggc ggcgacaccg gcaagcccgt
     3001 agccacgcta ggcgaagacg ccgccgctgc aaaaagtttg tacgccgtag cccgcgccgt
     3061 ttccgcccgc ctcgaagaac taggctcctc caccctcggc ccacaagtcc aaatcgacta
     3121 gcctctgcgc ttgcgcggcg actctggttt ctttctttgg gaggccggga cttcagcccc
     3181 ggcgtacagc gtgaaaacta gagagggctt tcacccctga ggctcacgag gcatggtccc
     3241 ggaattatcc cgacttgttt cccatcttcc atgtccactt ttctattttc tgctctccca
     3301 ttttccgtta tactcctcca tcgacgccgc cactcaacgc ggcgctgcgg caacccagtt
     3361 gccgcatttc gtgtttttgg agggcttgaa aggcgctata agtcgtctgg aatgaatact
     3421 tacgcgaaac cctctaaccc atttagaatt aatatttaca agaaacacga ggagagggtg
     3481 cccgctctgc ttaacccgcc tattaacaat cacttatgag cgcctcccgc cagcccgaca
     3541 tccgctccta ccgcggcaaa cacccgcaaa ttgccgcctc cgcctacgtt gatcccgctt
     3601 ccgtcatcat cggcgacgtc gtcatcggcg aagattc
//
LOCUS       DASTPM010000762         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1267471, whole genome shotgun sequence.
ACCESSION   DASTPM010000762 DASTPM010000000
VERSION     DASTPM010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267471"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2091
                     /locus_tag="VFR42_13475"
     CDS             <1..2091
                     /locus_tag="VFR42_13475"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEU0370222.1"
                     /translation="VQVELGRVSPGPDGQRAEGRIVRILNRAHPTVVGLFRYTPNGNV
                     VLPYDNRIQHQVEIPPGDELTSELRRKYGAAESREAGARPRRVGKVAELDGAVVNVEL
                     TRFPRGGISPIGRVIEILGKPGDLGVDTEIIIRKHHLPHTFSQEVLAETARRAHPVHE
                     NEWEGREDFRKLPIVTIDGETARDFDDAVYVERRSDGGWHLQVHIADVAHYVRTATPL
                     DNEARLRGTSVYFIDRAVPMLPESLSNGICSLNPREDRLVMSCLMEFDATGRMRDSRM
                     TAGLIRSAERMTYTNVNKVLQGDAEASARYAPVVHNFQDMKTLALLLNARRNEHGSID
                     FDLPEPVIEFDDEQRMVNITRSERNIAHRLIEEFMLAANRAVAGYLLRRGLATLHRVH
                     EKPDGKKVLEFEDTAKAFGYTLGVADLHQREVAVRHGRMPAPAKKGRPDSYGHGRERG
                     MRIQLPGNQEISITPQHYQRLLRKLAGKPEERIVSYLMLRSLKQARYAADPLGHFALG
                     FDEYAHFTSPIRRYPDLVVHRTLKWALAHPQQGGPVDPHPAAHAEAVVYSAKQLEELA
                     TETSDAERRAASAERELKDWKTAQFMEQHLGDEYEGLIISVQKYGCFVELLEVFVEGL
                     LPIGALEDAAGQRCVYRERDHAIVALGGAERSRSGRGAARAKPRAWGLGDRVKVRAER
                     IDPMRRRVEFALVS"
     gene            complement(2192..3556)
                     /locus_tag="VFR42_13480"
     CDS             complement(2192..3556)
                     /locus_tag="VFR42_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0370223.1"
                     /translation="MNPTENNSTTTRRTFLKVASAGIALNSTAASYAKILGANDRVRV
                     GLCGFSERFRDALLPAFREHATELNFEFAGVSDIWKLRRQEGVAHITKVTGSDVTPAR
                     NNDELFARKDIDAVFVATSDFQHAQHGVAAVRAGKDAYIEKPLANTMSDAKDIRNAVK
                     ETGRVVQIGTQRRSAENYQRAREFIGAGQFGDIVAVEMTWNVNQPGRWRRPSLVEQLR
                     EEDTDWKRWLGNRPSERFDPRKYVEFRLFWPYSSGIADQWMVHQIDTVHWFTGLPHPR
                     SVAANGGIYLWHDGRKNWDTMTAVFDYGPLNDPSKGFQVIYASRQTNSAGDVKELYRS
                     NGGTLDLDKNLISGDGGLEEQYAKAMGMKPNRLGNIPLVQKGGGMETGAHAGVDDATS
                     ANVRNWMECIRSRNIPNAHIEAGYSHSVALCMTIAAIQSGERVTFDDAKQQVLAGGSP
                     WPRG"
     gene            complement(3553..>3821)
                     /locus_tag="VFR42_13485"
     CDS             complement(3553..>3821)
                     /locus_tag="VFR42_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEU0370224.1"
                     /translation="GYALLPKNRIGHMHLKDVKRNADGTYEWECMGRGIIDYAEQFRA
                     MMRDGYRGTMSLETHWKGAGTMEESSRQAMAGTKELLRKAGALK"
BASE COUNT          727 a         1061 c         1243 g          790 t
ORIGIN      
        1 gttcaggttg aactggggcg cgtcagtccg ggtcctgacg ggcaacgagc ggaagggcgc
       61 atcgtccgca ttctgaatcg cgctcatccg acggtggtcg gactgtttcg ctacacgcca
      121 aacggcaacg tggttttgcc ctacgacaat cgcattcagc atcaggtgga gatcccgccg
      181 ggcgatgagc tgacctcaga gttgcgacgg aaatatggcg ctgcggaatc gcgagaagcg
      241 ggcgcacgtc cgcgacgtgt tgggaaagtg gcggagcttg acggcgcggt ggtgaatgtt
      301 gagctgacgc gatttccgcg cggaggaatt tctccgattg gccgggtgat cgaaattctg
      361 ggcaagcccg gcgatctcgg cgtggatacc gagatcatca tccgcaagca ccacttgccg
      421 cacacatttt cgcaagaagt gctggcggaa acggcgcggc gcgcccatcc agttcacgaa
      481 aatgaatggg aggggcgtga agattttcgc aagctgccga ttgtgacgat tgatggcgaa
      541 acggcgcgcg actttgacga cgcggtatat gtggagcggc gcagcgatgg cggctggcac
      601 ctgcaagtac acatcgcgga cgtggcgcac tacgtgcgaa cggcgacgcc gctggacaac
      661 gaagcgcggc tgcgcggcac ctcggtgtat ttcattgacc gggcggtgcc gatgctgccg
      721 gagtcgctgt cgaatggaat ttgttcgctg aatccgcgcg aagaccggct ggtgatgagc
      781 tgcttgatgg aattcgacgc gaccgggcgg atgcgcgatt cgcgcatgac tgccggtttg
      841 attcgctccg ccgaacgcat gacctacacc aatgtgaaca aagtgttgca gggtgatgcc
      901 gaagccagtg cgcgctacgc acccgtggtg cacaattttc aggacatgaa gacgctggcg
      961 ctgctgttga acgcgcggcg caacgagcat ggttcgatcg atttcgattt accggagccg
     1021 gtgatcgaat tcgacgacga acagcgcatg gtgaatatca cgcgcagcga gcgcaatatc
     1081 gcgcatcgcc tgattgaaga gttcatgctg gcggcgaacc gcgcggtcgc tggatatttg
     1141 ctgcggcgcg ggctggctac tctgcatcgc gtgcacgaaa agcccgatgg gaaaaaagtt
     1201 ttggagttcg aggacacggc caaggcgttt ggctacacgc tgggcgtggc ggatttgcac
     1261 cagcgcgaag ttgcggtgcg tcacgggcgg atgccggcgc cggcgaaaaa ggggaggccg
     1321 gattcgtatg gccacgggcg cgagcgaggg atgcgcattc aactgccggg caatcaggag
     1381 atcagcatca cgccgcagca ttaccagcgg ctgctgagaa aacttgcggg caagccggaa
     1441 gagcgcattg tgtcttattt aatgttgcgg tcgctgaagc aggcacgcta cgcggcggac
     1501 ccgctgggac attttgcgct gggctttgat gagtatgcgc attttacgtc gccaattcgc
     1561 cggtatccgg atttggtggt gcaccgcacg ctgaaatggg cactggcgca tcctcagcag
     1621 ggaggcccgg ttgatccgca tccggcggct cacgcggaag cggtggtgta cagcgccaag
     1681 caactagaag agctggcgac ggaaacttcg gacgcggagc gccgcgcggc ttcggcggaa
     1741 cgtgaattaa aagattggaa aaccgcgcag ttcatggagc agcatctggg cgatgaatac
     1801 gaagggctga ttatttcggt gcagaagtat ggctgcttcg tggagctgct ggaagtcttt
     1861 gtggaaggat tactgccgat cggcgcgctg gaagatgcgg ccggccagcg ctgcgtctat
     1921 cgcgaacgcg atcacgcgat tgtggcgctg ggaggagcgg aacgttcgcg cagcgggcga
     1981 ggcgcggcga gagcgaaacc gcgggcgtgg ggattgggag atcgggtgaa ggtgcgtgcg
     2041 gagcggattg acccgatgcg gcggcgcgtt gagtttgcgc tggtttctta gaaatcgaag
     2101 agagactcct cgctccgccg gccacggccg gcaagcccgg catgacgaat ttgggcacgt
     2161 cgctaaaccg ggcggcgcga gctgcgcatc gctacccgcg aggccagggg ctgccgccag
     2221 cgagtacctg ctgcttcgcg tcatcaaacg tgacacgctc gccggattga atcgcggcga
     2281 tggtcatgca cagcgcaacc gaatggctgt agcccgcttc gatgtgcgcg ttgggtatgt
     2341 tgcggctgcg aatgcattcc atccagttgc gcacgttggc gctggtggcg tcatccacgc
     2401 ctgcgtgtgc accggtttcc atgccgccgc ccttctgtac caggggaata ttgcccagtc
     2461 gattgggttt catgcccatg gctttggcgt attgctcttc gagaccgcca tccccactga
     2521 tcagattctt gtccaggtcg agcgtgccgc cgttcgagcg gtacagctcc ttcacgtcgc
     2581 ccgcggagtt cgtctgccgt gaagcgtaaa tcacctgaaa gcctttcgag ggatcgttga
     2641 ggggaccata gtcgaacacg gcggtcatgg tgtcccagtt tttgcggccg tcgtgccaga
     2701 gataaatccc accgttggcc gcgacactgc ggggatgcgg caaacctgtg aaccagtgca
     2761 cggtatcgat ctggtgcacc atccactgat cggcaatgcc cgaagagtac ggccagaaca
     2821 gccggaactc cacatacttg cgcgggtcaa atctttcaga ggggcggtta ccgagccagc
     2881 gcttccagtc ggtgtcttct tcccttagct gttccacgag cgaaggccgg cgccagcggc
     2941 cgggctggtt gacgttccat gtcatctcga cggcgacgat gtcgccaaac tggcccgcac
     3001 cgataaattc ccttgcccgt tgataattct ccgcgctgcg gcgctgcgta ccgatctgca
     3061 caacgcggcc ggtttctttt actgcattgc ggatgtcctt agcgtcgctc atcgtattgg
     3121 cgaggggttt ttcgatgtag gcgtcttttc cggcgcgtac tgcggcgacg ccgtgctggg
     3181 catgctgaaa gtcggatgtc gcgacgaaca ctgcatcaat atctttacgc gcgaaaagtt
     3241 cgtcgttgtt gcgagcgggt gtgacgtccg agcccgtgac tttcgtgatg tgcgcgacgc
     3301 cttcttgacg gcgcaacttc cagatgtcgc ttacgccggc aaattcaaaa ttcagctctg
     3361 tcgcgtgctc acgaaacgca ggaagcagcg cgtcgcggaa acgctcagag aatccgcaca
     3421 atccgactcg gacgcgatca tttgcgccga ggattttcgc gtaactggcc gccgtggaat
     3481 tcaaggcgat gcccgcactc gctactttca agaaggtccg ccgagtcgtg gtcgaattgt
     3541 tttccgttgg attcatttca gtgctcccgc tttgcgcaat agttctttcg tgccggccat
     3601 cgcctgacgc gaagactctt ccatcgtgcc ggcgcctttc cagtgtgttt cgaggctcat
     3661 cgtaccgcga taaccgtcgc gcatcatggc gcgaaactgc tcggcgtagt cgatgatgcc
     3721 tcgtcccatg cactcccact cataggtccc gtcggcgttt cgcttgacgt cctttaggtg
     3781 catgtggccg atgcggttct tcggcaacag cgcgtagcct t
//
LOCUS       DASTPM010000763         3255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_978796, whole genome shotgun sequence.
ACCESSION   DASTPM010000763 DASTPM010000000
VERSION     DASTPM010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978796"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VFR42_13490"
     CDS             complement(<1..1372)
                     /locus_tag="VFR42_13490"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU0370225.1"
                     /translation="MVRSLRRCLLGLLLLTPAAAFAQTSLEGTIHDVSGLPMANASIS
                     LQRQDGAKLNDATTDADGKFRFRALPAGAYALQVQAPGYFPLHYEFVLRPRQPLSLNL
                     DLQKAPTVQQSVEVHAGYLTIDPEKTGSSYTFTRQDLENLPDPLVETSNDLVNNLMPG
                     ASDSHDNFLAVRGTEFSLHEFINGVSFLDNTQPQFSPGVSPQIFETVDLMTGGFTPEY
                     GNRFGGVLDITTRSGAELGGHGDVNFRGATVDNFDLNADYGGQKGPLGYYFFIDGFTS
                     GRYLDPPEPRELFDFGKGSRATTQLDWHAGNKDTVRLLLMGSGANFQQPNITDDQDVG
                     RDAQRHLRQYTAILGWTHTFSPNTLLQTSLYQRSSSDHVLPTTDPITPLSDASRSGFA
                     LGVKSDLSHSWHGHFFKAGVDFVELREGESFFFDPRGDPDTFPAFQGWGKGGQASFYV
                     QDHFSPV"
     gene            complement(1663..2022)
                     /locus_tag="VFR42_13495"
     CDS             complement(1663..2022)
                     /locus_tag="VFR42_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370226.1"
                     /translation="MRNLGVLWVLYGLERLAVALAIFLCIPTLTVMWGALLTRVANPY
                     TLMDLFHLFLIFAIAFSILAGIISILAGSALTSARPSARTLGLLAAFLALVSGPLGIA
                     LGAYTLVVLVPANARTS"
     gene            2264..>3255
                     /locus_tag="VFR42_13500"
     CDS             2264..>3255
                     /locus_tag="VFR42_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370227.1"
                     /translation="MKPTLVFTLLGSLAMAGTASAQDKVFQWQPANDEAVRLDPANYH
                     TGRTYRPAPQGGNLHVDIEAQQPVTIFMTGEGEWNRALQYPESIGSIRKVCLREQVVK
                     TTYLCDLPGEPMTLVIRDDRGSRESAAFAGLGEVYKRDGALLSTPVGNAVGGAVGTGL
                     ALAKVFKSESSEKRHFVSPNDVHIQYYRWACIANCFPPEFQWIQQAKEKYELTSFLKV
                     YGGYAPDRDGELVSIKIKAPMPMLVAMVPSSVANQLHARPEMLEPALEKNSCQQRGVQ
                     SQTFQCTFNAADGPQSLIVVPEAGEKVKHKKVEIEFFASKCVEHCALPPDSTNAS"
BASE COUNT          708 a          770 c         1158 g          619 t
ORIGIN      
        1 gaaccggcga gaaatgatcc tggacataga agctggcctg gccaccttta ccccaaccct
       61 ggaatgccgg gaaggtgtcg gggtcgccgc gtggatcaaa gaagaagctt tcgccttcgc
      121 gaagttcgac gaagtcgaca ccggctttga agaaatggcc gtgccaggaa tgggagaggt
      181 cgctcttgac gccgagcgcg aagccagagc gtgaggcatc ggagagtgga gtgatggggt
      241 cggtggtggg caggacgtga tcggaactgc tgcgttggta gagggaggtc tgcaacaagg
      301 tgttcgggga gaaggtgtga gtccagccga gaatggctgt gtattggcgg aggtgacgct
      361 gcgcgtcgcg gcctacgtct tgatcgtcgg tgatattagg ctgctggaaa ttcgcaccag
      421 aacccattag aagcaagcgg acggtgtcct tgtttccggc gtgccaatcg agctgggtgg
      481 tggcgcgtga acctttgccg aaatcaaaga gctccctggg ttccgggggg tcgagatacc
      541 ggccggaagt gaagccgtcg atgaagaaat aatagccgag agggcccttt tggccgccgt
      601 agtcggcgtt gaggtcaaag ttatcgacgg tagcgccgcg aaaatttacg tcgccatggc
      661 cgccgagttc ggcgccggag cgcgtggtga tatcgagaac gccgccgaag cggttgccgt
      721 attcgggagt gaaaccgccg gtcatgagat ccacggtttc gaagatttgc gggctgacgc
      781 cgggcgagaa ttgcggctgg gtattatcga ggaaggagac accattgatg aactcgtgga
      841 gggagaattc ggtgccgcgg acggcgagga aattgtcgtg gctgtcgctg gcgccgggca
      901 tgaggttatt gacgaggtcg ttgctggttt cgacgagcgg atcggggaga ttttcaaggt
      961 cctggcgagt gaaggtgtag gagctgccgg ttttttcggg gtcgatcgtg agatagccgg
     1021 cgtggacttc cacgctttgc tggacggttg gcgccttctg gagatcgagg ttcagagaga
     1081 gaggctgccg agggcgcaga acgaactcgt agtgcagtgg aaaatagccg ggagcttgca
     1141 cctgcagggc gtaggcgcct gctggaaggg cgcggaaacg aaatttgccg tcggcgtcgg
     1201 tggtcgcgtc attgagcttc gctccatcct ggcgctgcag ggaaatagag gcattggcca
     1261 tggggaggcc ggagacgtca tggatggtgc cctcgaggga agtctgcgca aaggcggcag
     1321 ccggggtcaa caagagcaag ccgagcaggc agcggcgcaa cgaacgcacc ataatcagcg
     1381 aacccctaga ggaatgtagg cttgcggagc gaaggcccgc gagacgaaag agtctagcac
     1441 acgagtgaga cggagagcgt tactgcgagc ccgcaggagg cttttcgggg ttggtgacgg
     1501 aaatgacgcg agtagtctga ccgaagtcgc gtcttggagg ggcgatcttc ggagtagttt
     1561 tcttgggctt tttcttttcg cggctgcgcc tgtctttgtt tcccatggca gatagcatgc
     1621 cacggtcgcg gccagttggc tacagatgtc tgagcgtgac ggctaagagg ttctggcgtt
     1681 tgccggaacg aggacgacca gagtgtacgc gcccaaagca atgccgagag gcccgctaac
     1741 cagagcaaga aaggcggcca gtagaccgag tgttcgtgcg gaggggcgag cggaggtcag
     1801 ggcggaaccg gcgaggatag agatgatacc ggcgagaatg gagaaggcga tcgcgaaaat
     1861 caggaagaga tgaaataagt ccatcagcgt gtaggggttg gcgacgcggg tgagcagggc
     1921 tccccacatg acggtcaacg tgggaatgca aagaaaaatg gcaagagcga cggcaagccg
     1981 ctccaggccg tacagaaccc aaaggactcc gagatttcgc atggaaagct cccctgccag
     2041 gcttaacgtg catgcctggg cacggcggac aatgtacatc tgacgtcggc aagctgcaac
     2101 gagtgtggcc tggttttcgg gaggatggcg tccgctgaaa tggtgtccgg ggagcgcttt
     2161 gttgcccggt ggaatgtgct cagttggata cgatactcgt agagaggacc tgccctttcg
     2221 ggcaattgtg tgggtatttt cgctcggaaa tactgttatc actatgaaac ccacacttgt
     2281 gttcacactc ctgggcagcc tggcgatggc gggaacggct tcggcgcaag acaaggtttt
     2341 tcaatggcaa ccggcaaacg atgaggccgt ccggctcgac ccagccaact atcataccgg
     2401 gcggacgtac cggccagcgc cgcagggagg aaacctgcat gtggatatcg aggcgcagca
     2461 gccggtgacg attttcatga ccggggaagg cgagtggaac cgggcgctgc aatatccgga
     2521 atcgatcggg agcatccgaa aggtctgctt gcgtgagcaa gtagtgaaga cgacatattt
     2581 gtgcgatctt cccggagagc cgatgacgct ggtgattcgc gacgaccgcg gaagccggga
     2641 gtcggcggcg tttgcggggc tgggcgaggt atacaagcga gacggcgccc tactgagtac
     2701 gccggtcggc aacgccgtag gtggcgcagt gggaaccgga ctggctttgg ccaaggtctt
     2761 caagtcggaa agttcggaga agcgccattt tgtttcgccg aatgatgtgc atatccagta
     2821 ttaccgctgg gcttgcatcg cgaactgttt tccgccggag tttcagtgga ttcagcaggc
     2881 gaaggaaaaa tacgagctga cttcgttcct gaaggtgtat ggcgggtacg cgccggatcg
     2941 cgacggtgag ttggtgagca tcaagatcaa ggcgccgatg ccgatgctgg tggcgatggt
     3001 gccgtcgtcg gtagcaaacc agttgcacgc gagaccggag atgctggagc cggcgctgga
     3061 gaagaattcg tgccaacagc gaggcgtgca gtcgcagaca tttcagtgca cgttcaatgc
     3121 ggctgacggc ccgcaatcac tgattgtggt gccggaggcg ggcgagaagg tgaagcacaa
     3181 gaaagtggag atcgagtttt ttgcgtcgaa atgcgtggag cattgcgcgt tgccgcccga
     3241 cagcacgaat gcctc
//
LOCUS       DASTPM010000764         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_460006, whole genome shotgun sequence.
ACCESSION   DASTPM010000764 DASTPM010000000
VERSION     DASTPM010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460006"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VFR42_13505"
     CDS             <1..580
                     /locus_tag="VFR42_13505"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370228.1"
                     /translation="GANVDSKPEHLVQFAIMGEMYYRATFGHRRPRVGLLSIGEEESK
                     GNELTREVHERLKKAHLHYIGNVEGGDLFSGAVDVIVCDGFVGNIALKICEGLAVQIF
                     GLLRKSLKSSLVSQFGAMLSQGAFKGLKKTIDYTEYGGAPLLGVRGVCVIGHGRSNAN
                     AVKNAIRVAAGLAKSRMNEKIEEELSLVTERV"
     gene            724..1677
                     /gene="fabD"
                     /locus_tag="VFR42_13510"
     CDS             724..1677
                     /gene="fabD"
                     /locus_tag="VFR42_13510"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU0370229.1"
                     /translation="MGKVAFVFPGQASQYPGMGKELAEKYAVARAVFEEADKALGFSI
                     SKMCFEGSEEELKQTANTQPAILTCSVAAFRVAAEKGLQPDFVAGHSLGEYSGLVAAD
                     GLKFADAVQLVRKRGQYMQDAVPAGVGAMAAILGLSPAVVADACKRAAENEICTPANL
                     NSPEQTVISGHAGAVKRAVEIASQLGAKRAVILPVSAPFHCALMMPAQEKLANDLEKF
                     AFSDLRVPLVTNVDADTEAKGSEAKDALVRQVSMPVRWEESVHSLIDEGVNTFVEVGP
                     GRVLTGLLRQIERSVAALNIDDEKSLATTLEKIAGAKSDAA"
     gene            1716..>2221
                     /locus_tag="VFR42_13515"
     CDS             1716..>2221
                     /locus_tag="VFR42_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0370230.1"
                     /translation="MFSLKDKVAVVTGASQGIGRDTALALTEAGAKVVVAARNEEKLT
                     ALVSDIAAGGGEAFAIKMDVADAEQVKAGFKQVIEKFGRLDILVNNAAVTRDGLAMRM
                     KKEDWETVLQTNLTGAHFCVQQVLPVMMKARAGRIINISSVVAQSGNAGQANYVAAKA
                     GLIGLTKA"
BASE COUNT          498 a          514 c          773 g          436 t
ORIGIN      
        1 tggcgcgaat gtggactcga agccggaaca cctggtgcag ttcgcaatta tgggggaaat
       61 gtactaccgc gcaacctttg gccaccggcg gccgcgcgtg gggctgctgt cgatcgggga
      121 agaagagagc aagggcaacg agctgacgcg agaagtgcac gaacggctga agaaagcgca
      181 tctgcactac atcggcaacg tcgaaggcgg agaccttttt tctggcgcgg tggatgtgat
      241 tgtctgtgat gggtttgtag gaaatattgc gctgaagatt tgtgaaggcc tggcggtgca
      301 aattttcgga ttgttgcgca agtcgttgaa aagttccctg gtgtcccagt tcggcgccat
      361 gctttcgcag ggcgcgttca aggggttgaa gaagacgatc gattacacgg agtacggcgg
      421 cgcgccgctg ttaggcgtgc gcggggtctg tgtaatcggc catgggcgct ccaacgcgaa
      481 cgcggtgaag aatgcgattc gcgtggcggc gggcctagcc aagtcacgca tgaacgaaaa
      541 gattgaggag gagctgtcgc tggtgacgga gcgtgtgtga agcgttccat cgtcattccg
      601 agcggagcgg gaaatcactc ctgcctggga tgatgaaata agagagattc ctcgctccgc
      661 ggactccgct cggaatgacg gatgtggggc gggagctgcc aagaacagcg aagagagaag
      721 cgtatgggaa aagtggcatt tgtttttccg gggcaggctt cgcaataccc gggaatgggg
      781 aaagagttag cggagaagta cgccgtagcc cgcgccgttt ttgaagaagc ggacaaagcg
      841 ctgggatttt ccatttcgaa gatgtgcttt gaaggctccg aagaagagtt gaagcagacg
      901 gcaaacacgc agccggcgat tttgacttgt tccgtagcgg catttcgcgt ggccgccgaa
      961 aagggcctgc agccggactt tgtggcgggt cacagcctgg gtgagtactc ggggctggtt
     1021 gccgcggacg gattgaagtt tgccgatgca gtgcagttgg tgcgcaagcg cggccagtat
     1081 atgcaggacg ccgtgccggc gggagttggc gccatggccg caattttggg attgtcgcct
     1141 gctgttgtgg cggatgcctg caaacgcgcc gccgagaacg agatttgtac gccggcgaat
     1201 ttgaactcgc cggagcaaac cgtaatttcc ggccacgccg gtgctgtgaa gcgcgcggtg
     1261 gagattgcgt cacaactcgg cgctaagcgc gctgtgattt tgccggtgtc tgcgccgttc
     1321 cattgcgcct tgatgatgcc ggcgcaggag aaactggcga acgatttgga gaaatttgcg
     1381 ttttcggatt tgcgtgtgcc gctggtcacg aacgtcgacg cggacacgga ggctaagggt
     1441 agcgaagcga aggatgctct ggttcggcaa gtctcgatgc cggtgcgctg ggaagagtcg
     1501 gtgcactcgt tgatcgacga aggagtgaat acctttgtag aagtcgggcc gggccgtgtg
     1561 ctgaccggct tgctgcggca aatcgagcgg tcggtggcag cgctaaacat cgacgacgaa
     1621 aaaagtttgg cgacgacgtt ggagaaaatc gcgggagcaa aatcggacgc agcttaggat
     1681 ttcaaccaca gagtgcacag aggaagcaga gaagaatgtt tagcttgaaa gataaagtag
     1741 ccgtggtgac gggtgcgtcg cagggaatcg gacgcgacac tgctctggca ttgaccgaag
     1801 cgggcgcaaa ggtagttgtc gcggcgcgta acgaagaaaa actgacggcg ctggttagcg
     1861 atatcgctgc ggggggcggg gaagcatttg cgatcaagat ggacgtggcc gacgcggaac
     1921 aggtgaaggc tggcttcaag caagtaatcg aaaaatttgg ccgcctcgat atcctcgtca
     1981 acaacgccgc cgtaacgcgc gacggcctgg ccatgcgcat gaagaaagag gattgggaaa
     2041 cggttctaca gacgaatttg accggcgcac atttttgcgt ccagcaagtg ctgcccgtga
     2101 tgatgaaggc gcgcgcggga cggatcatca acatttcctc agtcgtagcg cagagcggca
     2161 acgctgggca ggcaaattac gttgctgcga aggcgggcct gatcggatta acgaaggcga
     2221 t
//
LOCUS       DASTPM010000765         3475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1151815, whole genome shotgun sequence.
ACCESSION   DASTPM010000765 DASTPM010000000
VERSION     DASTPM010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151815"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..525)
                     /locus_tag="VFR42_13520"
     CDS             complement(88..525)
                     /locus_tag="VFR42_13520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0370231.1"
                     /translation="MEFHDATVRVRYAETDQMGVVYHANYLIWFEIGRVELMRALGFE
                     YKLMETEDDCYIVVAEASCRYLHPARYDEVLRVRTRISRAGNRVVKYAYELLRDSDNA
                     VLATGATTHVICGSDGKPKQLPEKYRDKFRALADKNNEKNSTP"
     gene            complement(638..1420)
                     /locus_tag="VFR42_13525"
     CDS             complement(638..1420)
                     /locus_tag="VFR42_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0370232.1"
                     /translation="MTFSTLLLDYGSERATITLNRPDKRNAINPAMISELQTAFDEIE
                     KKKVRVVIMTGAGAAFCAGMDLDMLQAIAKQSPQENQEDSKRMAKLFRRIWSFPRPII
                     AAVNGHALAGGCGIATLCDFTLAVPEACFGYTEVRIGFLPAIVSVFLTRQIGDKRARD
                     LLLTGRKVSAKEAQQLGLVSEIVEPEKLVTRANELADYLVEVSPGSLSRAKRLLVTAE
                     QAGVDADLERAVLENARIRCTPDFQEGIASFLEKRKPVWKDE"
     gene            complement(1459..2355)
                     /locus_tag="VFR42_13530"
     CDS             complement(1459..2355)
                     /locus_tag="VFR42_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370233.1"
                     /translation="MEDKTTLIECPRDAWQGLPEIIPADYKADYLRELVLAGFRHVDA
                     VSFVSPKHVKQMADSEEVMQKFLASLPANAEPPEIIGIVVNEKGLERALATPGVTTIG
                     YPYSISAYFRRANANMTRGESRTLVETLNKTSKAANKGLVIYISMAFGNPYDEPWGPE
                     IVEETLEWLKDIRVKTVSLADTAGNATPELIADLFQRANAAVAGIELGVHLHSRPQNA
                     AGKIIAAYSAGCRRFDGALTGLGGCPFAGDELVGNIRTEDILAAVKKAGGETGIDEAK
                     LGKVLAMTNEIRTKYGHAPIAS"
     gene            complement(2674..>3475)
                     /locus_tag="VFR42_13535"
     CDS             complement(2674..>3475)
                     /locus_tag="VFR42_13535"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU0370234.1"
                     /translation="YGIFSSDAGKQYDMREIIARMVDASEFEEYRAEYGETLLCGYAR
                     IGGWAVGIVANQKKHVQTTAPGSDQKRIEFGGVIYTESAEKAARFILDCNQSRIPLIF
                     LHDVNGFMVGKDAEWSGIIRAGAKMVNAVSNSVVPKISVICGGSFGAGHYAMCGKAYD
                     PRFIFAWPTARYAVMSGDAAASTLAEIKLKQLEREGKKIDEKEKKKLYESTRATYEHQ
                     ADPRYAAARLWVDAIIDPAKTRDSLIWALEAAALNSHLPEFKTGVLQT"
BASE COUNT          706 a          890 c         1107 g          772 t
ORIGIN      
        1 atgcggaata aaagagtaac acagaggaag cgaaggattc acgtaagggc gcaggcaacc
       61 aagcaagaaa aggtgtgctt cgttctttca tggcgtcgag ttcttttcgt tgtttttgtc
      121 tgctaaggcg cggaatttgt cgcggtattt ttcgggaagt tgtttgggct tgccgtcgct
      181 cccgcagatg acgtgagtgg tggctccggt ggcaagcacg gcgttgtcgg agtcgcgcag
      241 aagttcgtag gcatatttca cgacgcgatt gccagctcgc gagatgcggg tgcggacgcg
      301 gaggacttcg tcatagcgcg cgggatgcag atagcggcag ctggcttcgg cgaccacgat
      361 gtagcagtcg tcctcggttt ccatgagctt gtattcaaag ccgagggcgc gcatcagttc
      421 gacgcggccg atctcgaacc agatgagata gttcgcgtga tagaccacgc ccatttggtc
      481 ggtttcggca tagcggacac gtacggtggc gtcatgaaat tccatgcaag ctccttggag
      541 caccaggcaa aagcagattc ctcgtcactt cgatttctcg gaatgaccag gaaggcgagc
      601 ggaaaaagaa caaagagtcg atactaggaa cggccaacta ttcgtctttc cagacgggtt
      661 tgcgtttttc caggaaggag gcgatgcctt cctggaagtc gggcgtacag cggatgcgcg
      721 cgttttcaag aacggcgcgt tcgaggtcgg cgtcgacccc ggcttgttcg gcggtgacga
      781 gaaggcgctt ggcgcggctg aggctgccgg ggctgacttc gaccaaataa tcggcgagtt
      841 cgttcgcgcg ggttacgagt ttttccggtt cgacaatttc gctgaccagg ccgagttgct
      901 gcgcttcttt ggcggaaact ttgcggccgg tgagcaggag atcgcgagcg cgcttgtcgc
      961 cgatctgacg tgtaagaaaa acggaaacga tggctggcag gaagccgatg cggacttcgg
     1021 tgtagccgaa acaggcttcg ggaacggcca aagtgaaatc acagagtgtg gcgatcccgc
     1081 agccgccagc aagcgcgtgg ccgttcaccg cggcgatgat cgggcgagga aagctccaga
     1141 tgcggcgaaa aagtttggcc atgcgcttgg agtcttcctg attttcctgg ggcgactgct
     1201 tggcgatggc ctggagcatg tcgagatcca tgccggcgca gaatgcagcc ccggcgcccg
     1261 tcatgatgac cacgcgaact tttttctttt cgatctcgtc gaaggcggtt tgcagttccg
     1321 agatcatggc gggattgatg gcgttgcgtt tgtcggggcg attgagcgtg atggtggcgc
     1381 gctcgctacc gtagtcgaga agaagcgtgg aaaatgtcac agggcctcca ttcggtgaag
     1441 gtttacaccg caaacaggct aacttgcaat cggggcatgg ccgtacttcg tgcgaatctc
     1501 gttggtcatg gccaggactt tgccgagctt tgcttcgtcg atgcccgttt cgccaccggc
     1561 ttttttgacg gcggcgagaa tgtcttcggt gcgaatgttg ccgacgagct cgtcacctgc
     1621 gaaggggcag ccaccgaggc cggtaagagc gccatcgaaa cggcggcagc cggcgctgta
     1681 ggcggcaata attttgcctg cggcattttg cgggcggcta tgcaggtgca caccgagttc
     1741 gatgccagcg acggcggcgt tggcacgttg aaagaggtcg gcgatgagtt cgggagtggc
     1801 gttaccggca gtgtccgcga gagaaacagt tttgacgcga atatctttca gccattcgag
     1861 agtttcttca acgatttcgg gtccccaggg ctcatcgtac ggattgccaa acgccataga
     1921 gatgtaaatg accagacctt tgttggcggc tttgcttgtc ttgttcaggg tttcgacaag
     1981 cgtgcgcgat tcgccgcgcg tcatattggc gttggcgcgg cggaaataag cggagatgga
     2041 gtacgggtag ccgatcgtgg tgacaccagg agtggcgagc gcgcgctcga gtcccttttc
     2101 gttgacgacg attccaatga tttctggcgg ttccgcgttc gccggaagag aagcgaggaa
     2161 cttctgcatg acttcctcgc tatcggccat ttgcttgacg tgtttcggcg agacgaagct
     2221 gacggcgtca acgtgccgga agccagccag aacaagttcg cgcaaataat cggctttgta
     2281 gtcggccgga atgatttccg ggaggccttg ccacgcatca cgcgggcatt cgatgagggt
     2341 cgttttgtct tccatttggt ctctaaacga agggattttt caccaatttt acggcgacgc
     2401 cagcaatccg acaaacccga tctccttgat aagcagcaag aggatcgcct acttatgcgg
     2461 cgcccggcgg ctaaaactac cgaaaccaga aaaactgtgg cgctccaagc agtccgacag
     2521 aatcagcgaa tccggccaat aaccgccagg cacggccagg attcctttgg ctcaacaaag
     2581 acaagataag gcggaccaag gaaggcaaaa gcagaacacc ggcgaaccac atgacgcgtt
     2641 gctcggtctg gttaggcggg ggcgccagag tcgtcacgtt tgcaagaccc ctgttttgaa
     2701 ttccgggaga tgcgagttga gggcggcggc ttcgagggcc cagattaatg aatcgcgggt
     2761 tttggcaggg tcaatgatgg catcgaccca aagacgggcc gcggcgtaac gcgggtcggc
     2821 ctggtgttcg taagtggccc gagtcgattc gtagagcttc tttttttcct tttcgtcgat
     2881 cttcttgccc tcgcgttcca gttgtttaag tttgatttcg gcgagcgtgc tggcagcggc
     2941 gtcaccgctc attacggcgt agcgtgcggt gggccaggca aaaatgaagc gcgggtcgta
     3001 ggctttgccg cacatagcgt agtggccggc gccaaagctg ccgccgcaga tcacggaaat
     3061 tttcggcacc acactattgg acactgcgtt gaccattttt gcgccggcgc ggatgatacc
     3121 ggaccattcg gcgtctttgc cgaccatgaa gccgttcacg tcgtggagaa agatcaaagg
     3181 gatgcggctc tgattgcagt cgaggatgaa ccgggcagct ttttcggcgg attccgtgta
     3241 gatgacgccg ccgaactcga tgcgtttctg atcggagccc ggcgccgtgg tctgtacatg
     3301 ttttttctgg ttggcgacga tgccgacagc ccacccgccg atgcgtgcgt agccgcagag
     3361 aagcgtttcg ccgtattcgg cacggtactc ctcgaactcg ctggcgtcga ccatgcgcgc
     3421 gattatttcg cgcatgtcgt attgcttgcc ggcatcgctg gaaaagattc cgtag
//
LOCUS       DASTPM010000766         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_56544, whole genome shotgun sequence.
ACCESSION   DASTPM010000766 DASTPM010000000
VERSION     DASTPM010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56544"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFR42_13540"
     CDS             complement(<1..289)
                     /locus_tag="VFR42_13540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0370235.1"
                     /translation="MKPPYVIREFRRTDFSRLWEIDQKCFPTGISYSEPELLFYIQRP
                     RAFTLVAERSGEEGQAVGFIVAEIGRQMGHIITIDVLEQERRGGLGSQLIVA"
     gene            455..1108
                     /locus_tag="VFR42_13545"
     CDS             455..1108
                     /locus_tag="VFR42_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370236.1"
                     /translation="MGRLLLIDVLGAIVSLAIWYYAFARYNRRRGAAALRWVQLACRG
                     RGKVVESCWSGSSRLQARFQFPSRWFENARLTLDLRPRPLPVQWLLSHWRKQKETLTF
                     EADLDCPPNFRLEVMNHRWCGHSNGKRANDNRAWEISRPGPVVLTTRAQWTQELTPVV
                     NALMASREHNFLKVHFRQDSPNFSATVDLETLADAEAASGFFTVLRELAAGASAKQL"
     gene            complement(1478..>2328)
                     /locus_tag="VFR42_13550"
     CDS             complement(1478..>2328)
                     /locus_tag="VFR42_13550"
                     /inference="COORDINATES: protein motif:HMM:NF023746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU0370237.1"
                     /translation="AVVTKPFAFEGKRRMQQAERGVAELMDSVDTTIVIPNEKLLAVA
                     QDAGFFESFRVADDILRQGVQGISDIITIPGIINRDFADVKAIMAAMGYAVMGAATAS
                     GDHRATDAAQKAIASPLLEAGAIDGARGILINITGSNSLKLAEVQQACTIIQSAAHED
                     ANIIFGAVLDEKMKDSVKITVIATGFREVPAMRHRQQEVQPTFASVHNNHDYSEPEPA
                     PFEPEPLPERIMKDDSLPAEPEAGMQQVSPEVASLDALRTAMVSNFEQNDLDVPAFLR
                     KRGDVM"
BASE COUNT          449 a          717 c          668 g          494 t
ORIGIN      
        1 cagcaacgat cagctgtgac ccgagcccgc cgcggcgctc ctgctccaaa acgtcaatcg
       61 tgatgatgtg ccccatctgc ctgccgattt cggccacgat aaatccaaca gcctgccctt
      121 cttcgcccga gcgttccgcc accagcgtga aggcacgtgg cctctgaata taaaagagca
      181 attccggttc ggagtacgag attcccgtag ggaaacactt ctggtcgatt tcccacagtc
      241 gggaaaagtc ggtccgccga aattctcgaa taacatatgg cggtttcatc gcatccaaga
      301 cgcgtccgga tagagtgtaa tgcccgctcg cagacgccgg ctgccgggtc ttttgaccgc
      361 cgaccactcc agaggggcaa tcagaaaatt tacgcgcctg gctcttgccc tctctccgcc
      421 gtcggacgta ccatctgagt aaacgaggtc ctctgtgggg cgcctgttat tgatcgacgt
      481 gttaggtgcc atcgtctcct tggccatctg gtactacgcc tttgcgcgct acaaccggcg
      541 gcgcggggcg gccgccttgc gctgggtgca gcttgcctgc cgcggccgcg gcaaagttgt
      601 agagagttgt tggtccggca gttcccgtct ccaagcccgc ttccagtttc cttccaggtg
      661 gtttgagaat gcccgcctca ccctcgacct ccgcccgcgc ccgttacccg ttcaatggct
      721 gctgagtcac tggcgcaagc aaaaggaaac gctcaccttc gaagccgacc tcgattgccc
      781 gcccaatttt cgcctcgaag tcatgaatca ccgctggtgc ggacacagca acggcaagcg
      841 cgctaacgac aatcgcgcct gggaaatttc ccgccccggc ccagtcgtgc tgaccacacg
      901 cgcccagtgg acccaggaac tgacgccggt ggtcaacgcg ctgatggcgt cgcgcgagca
      961 caacttcctg aaagtgcatt tccgccagga ctcgcccaac ttctccgcca ccgtcgatct
     1021 tgagacgctc gctgacgcgg aagcggcctc gggcttcttt accgtgctgc gcgaactggc
     1081 agccggtgct tccgccaaac agttatagta tcctgcattt gttttcaaga acatagccat
     1141 aagtaataga aaaataccag tctgcggaca gaggtaatct ttcaagaata gggtgggagt
     1201 ctcactcaga ataccatttg ctggcgttac gaggccctag gcagcgcgtt tgagggtccg
     1261 atagacctgg ttagggcgcg acggaaagga tgcgcctgag ggcttctatt gcggaaacct
     1321 gccgttcggc tatacatgca tggaataccg ctggatctgt ttacccttaa agatatagta
     1381 ccaggagtgg aaagcggggg tattctcgtc atttccttcc aaagagatag cggccgggca
     1441 ccttgcctcc ggccgccgaa acgcagaaaa atatcagtta catgacgtcg ccgcgttttc
     1501 gcagaaacgc cggcacatcc agatcgttct gttcgaaatt cgacaccatc gccgtacgca
     1561 aggcgtcgag ggacgccact tccggcgaca cctgctgcat gccggcctcg ggctccgccg
     1621 gcaggctgtc gtctttcatg atccgctccg gcagcggttc cggttcgaag ggcgctggct
     1681 cgggctcgga gtagtcgtgg ttgttgtgca cgctggcaaa cgtaggctgc acttcctgct
     1741 ggcggtggcg catggccggg acttcgcgga agccggtcgc gatgaccgtg atcttcacgc
     1801 tgtctttcat tttttcgtcg agcaccgccc cgaagatgat gttcgcatcc tcatgggccg
     1861 cgctttggat gatggtgcag gcctgctgga cttccgccag tttcagcgag ttcgagccgg
     1921 tgatgttgat caggatcccg cgtgcgccgt cgatcgcccc agcttccagc agaggagatg
     1981 caatggcttt ctgcgccgca tcggtagccc gatgatcgcc gctcgcggtc gccgcgccca
     2041 tcaccgcgta acccatggcc gccatgatgg ctttcacgtc ggcgaagtcg cggttgatga
     2101 tgccgggtat ggtgatgatg tcggaaattc cctgcacgcc ctggcgaaga atgtcatcgg
     2161 ccacgcggaa ggactcaaag aagccggcat cctgggccac cgcaagaagc ttctcgttcg
     2221 ggatgacaat cgtcgtgtcc accgaatcca tgagttccgc gacgccgcgc tctgcctgtt
     2281 gcatgcggcg cttgccttcg aatgcgaacg gcttggtgac aacggcga
//
LOCUS       DASTPM010000767         4233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_921579, whole genome shotgun sequence.
ACCESSION   DASTPM010000767 DASTPM010000000
VERSION     DASTPM010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921579"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..2157
                     /locus_tag="VFR42_13555"
     CDS             205..2157
                     /locus_tag="VFR42_13555"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU0370238.1"
                     /translation="MAGQTNTGAAVSAKPQPVAMEIPVTVNGASTVAGSEKREPFSET
                     TQTVLVFGSGAVIRLAAAVGPGQLLFVTNEKSKQEVVCQVVKTKQNGSTGGYVELKFT
                     EATTDFWGIRTPDNGSGSVASAGSIAGVPVKSLEQRLADLKTATPAATQITPPPIAPP
                     PAPTPVASAPPIAALNPTPIWAPAAKANEIPAAPGMPAPLPASRVPTLSEFLTHGSNG
                     PELKAAEKGSPGKNGNREATPAAVLTKEKPVPVSTAPSQVSARDDQKPSLSAALGVSQ
                     SVAPGAASFDLSADLPTEELKIPAWLEPLARNASISENKPTEAKLAEAKLPVPVNAGA
                     AQNEEASVTATISADIAQDVEAVPVLATEGRSPNFGTSLSLGSQEEGAPSSGNGWKIG
                     LSIAALLLAAAAIWYWYVNQPPKVSAGGNSTVAGTNPESIPAPAPSMPASEPVRASTV
                     TPTANRSAESAPLSQPAPSTASSTRPAGNSSSALKDTSNVSAYDAPMEPAKKPALGEV
                     RLAAPKVARKNQAEESGAADPGLALNGVTAADSSSLSILTNKSKGPAAPLPVGGDVKV
                     ARLLSSVPPVYPQMARAQRVSGDVTIDALIDVNGRVSATRVISGPALLHEAAMGAVRQ
                     WKYQPATLNGIPTATHLTVTVQFRLQ"
     gene            2450..3721
                     /locus_tag="VFR42_13560"
     CDS             2450..3721
                     /locus_tag="VFR42_13560"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU0370239.1"
                     /translation="MSQATAPEEMNVYQNAEARFEEAAKKLGLEQGVYKYLKYPDKEV
                     TMYIPVGMDNGTLEVFTGYRVLHSTVRGPGKGGIRFAPDVSLDEVRALAAWMTWKCAV
                     VDIPFGGAKGGVICDPRKMSRGELERLTRRYTAELTSWLGPERDVPAPDVGTNEQTMA
                     WVMDTYSMHVGMATTAVVTGKPIELGGSRGRKEATGRGVMICVDQAIEKLRMKKEGCR
                     TVIQGFGNVGSMAADLMQKAGYKIIGLADIGGGLYNENGFDIPKVIDWVTNQKKPLQD
                     FPGGGQKMSAKDVLFQPCEVLIPAAIENQITEKNAANVHAKILCEGANGPTTWQADAI
                     LDRKGVFTVPDILGNAGGVTVSYFEWVQDRQGFFWRESEVNERLMDIMVNAFDVVVKY
                     AEKHKVNNRIAAYMVAIDRVAKALKMRGIYA"
     gene            3837..>4233
                     /locus_tag="VFR42_13565"
     CDS             3837..>4233
                     /locus_tag="VFR42_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0370240.1"
                     /translation="MSPDSVLAGNQKSEKQHRRDICSVGRWMYQREYIVACEGNVSVR
                     LSDGRILTTPTCMNKGLLEPADLVVIDPDGRQIEGTRRTSSEVLMHLLFYKMRPDVRA
                     ICHAHPVTATGFAAAGRALDEALLPEVVIG"
BASE COUNT          966 a         1251 c         1291 g          725 t
ORIGIN      
        1 acgctgactg aaagctgccg cccatacctg tacgtgcgga caggtgtttc caccgtagta
       61 cgcgcgtact atcgtcggac tcccttggga ccaccagcct atataaggat agtcggcgtg
      121 gaattttgcc attccaaaga ccgtccgaaa aatttcgaca gggcaagcag gtacgagatg
      181 gctccactca cgagagatcc tgaaatggca ggacagacga acaccggcgc agcggtcagc
      241 gcgaagccac aaccggtggc catggaaatt ccggtgactg taaacggcgc gagcaccgtt
      301 gcgggcagcg aaaagcgaga gccattttca gagacgacac aaaccgtgct ggtattcggc
      361 agcggagcgg tgattcgcct tgccgcggca gttgggccgg gccagttgct ctttgtcacc
      421 aacgaaaaat ccaagcaaga agtggtttgc caggtagtga agaccaagca gaacggcagc
      481 accggcggat acgtcgagct gaaatttacg gaagccacca cggatttttg gggaattcgc
      541 acgccggaca atggctcagg tagcgtcgca tccgctggct caatcgccgg cgtcccggtg
      601 aagtcgctgg agcaaaggct ggcggacctg aaaaccgcaa ctccagccgc cacgcaaata
      661 actccgccgc cgatcgcgcc accgcccgca ccaacgccag tggcctccgc gccgcccatc
      721 gccgcgctca atcccacgcc aatctgggcg cccgctgcga aggcaaacga gattccagcc
      781 gcgccgggga tgcccgcgcc gcttcccgca tccagagtcc cgacgctttc ggaatttctt
      841 acgcatggtt cgaatggtcc cgaactcaag gccgccgaaa aaggatctcc aggaaaaaat
      901 ggaaatcgcg aagccacacc ggcagccgtg ctgactaaag aaaagcccgt ccccgtaagc
      961 accgcgccgt cgcaagtttc agcgcgagac gatcaaaagc cctcgctgag cgcggccttg
     1021 ggtgtaagcc aaagtgtcgc gccgggcgcg gcttcattcg atttgtccgc ggatctgccc
     1081 acggaagaac taaagattcc ggcgtggctg gaacctttgg cgcgcaatgc ttccatctcg
     1141 gaaaataagc caaccgaagc caagctcgcc gaggccaagc tgcccgttcc cgtgaatgcg
     1201 ggggcggctc agaatgaaga ggcttccgtc actgcgacca tatcggccga catcgctcaa
     1261 gatgtggaag ctgtgcctgt gctcgcaacc gaaggccgct cgccgaattt cggcacgtcg
     1321 ttgtcgctag gttcgcagga ggaaggcgcg ccgagttccg gcaacggctg gaaaatcgga
     1381 ttgtcaattg cggcgttgct gctggcggca gcggcgatct ggtattggta tgtgaatcag
     1441 ccgccgaaag tttccgccgg cggaaattcg acggtagccg gaacaaatcc ggagagcatt
     1501 cccgcgccgg cgccgagcat gcctgcttcc gagcccgtgc gagcgagcac ggtgaccccc
     1561 accgcgaaca ggtcggctga gtccgcgccc ctttcgcagc ccgcccctag caccgcaagc
     1621 agcactcggc cagctggaaa tagctcctcc gccttgaaag acacgtcaaa cgtttccgcg
     1681 tacgatgcac ccatggagcc cgcaaagaag cccgcgctcg gcgaagtccg cttggctgcg
     1741 ccgaaagtcg cgcgcaaaaa ccaagccgag gagagcggcg ccgcggatcc aggactggca
     1801 ttgaacggag tgaccgctgc ggattccagc agcctgagca ttctgaccaa caaaagcaaa
     1861 ggccctgctg cgccgctacc ggtcgggggc gatgtaaagg tcgcccggct gctgtcttca
     1921 gtgccgcctg tgtatccgca gatggcgcgc gcccagcggg tgagcggcga cgtgacgatc
     1981 gatgcgctga tcgacgtgaa cgggcgcgtt tccgccacgc gcgtaatttc cgggcccgcg
     2041 ctattgcatg aagccgcgat gggcgccgtg aggcaatgga aatatcagcc ggcaactctg
     2101 aacggtattc ccaccgcgac ccacctcacc gtaaccgtgc agttccggct gcaataacgc
     2161 acacattcac actggctggc gccacctata tcagtcattt ctcgagcggc gtatgcaatc
     2221 ctggaacgaa cctccggcga cccggcttgc ggcgacacaa cgcggcttcg tatactggca
     2281 ctccgtcccg gtgcacaact ggcaggtggg tagcctgctg aaaggtaacg ctaccgggcg
     2341 attcgaggaa aaatccgcaa ttgagtttgc ggcggggtgt gccagccagc acggtgatgc
     2401 gggcgggccc gacgcgccga gactccgcgc tcctcgcaga gaggccagaa tgtcgcaagc
     2461 gactgcaccg gaagagatga atgtatacca gaacgcggaa gcgcgttttg aagaagctgc
     2521 gaagaagctg ggcctcgaac aaggcgtcta taaatacctc aaatatcccg acaaagaagt
     2581 gaccatgtac attccagtag gaatggacaa cggcacgttg gaagtgttca ccggatatcg
     2641 cgtgctgcac tcgacggtgc gcgggcctgg caagggcggc attcgtttcg cacccgatgt
     2701 cagccttgat gaagttcgag cgttggccgc gtggatgacc tggaagtgcg cggtggtgga
     2761 cattccgttt ggcggcgcga aaggcggcgt gatttgcgat ccgcgcaaga tgtcgcgcgg
     2821 cgagctggaa cggctaacac ggcgctacac tgcggaactg accagctggc ttggcccaga
     2881 gcgcgatgtg cccgcgcccg acgttggcac caacgagcag actatggctt gggtgatgga
     2941 tacctactcc atgcacgtcg gcatggctac gaccgcagtc gtaaccggga aaccaatcga
     3001 actgggcggc tcgcgcggac gcaaggaagc caccgggcgc ggagtgatga tctgcgtcga
     3061 tcaggccatc gaaaaattga gaatgaaaaa agagggatgc cgtaccgtca tccagggttt
     3121 cggcaacgtt ggctccatgg ctgcggacct catgcaaaag gccggataca agattatcgg
     3181 gcttgcggat atcggcggcg ggttgtacaa cgaaaacggc ttcgacattc ccaaggtgat
     3241 cgactgggtg accaaccaga agaaaccgct gcaggatttt ccgggcggcg gtcagaagat
     3301 gagcgccaag gacgttctgt tccagccttg cgaagtgctc atcccggcgg ccatcgagaa
     3361 ccagatcacc gaaaagaatg cggccaacgt ccacgccaag attctctgcg agggcgcaaa
     3421 cggtcccacg acgtggcaag ccgatgcgat cctggaccgc aaaggcgtgt tcaccgtgcc
     3481 ggacattctc ggcaatgcgg gcggcgtaac cgtcagctac ttcgaatggg tgcaggaccg
     3541 ccagggcttc ttctggcgcg aaagcgaagt caatgaacgc ctgatggaca tcatggtgaa
     3601 cgcgttcgat gtagtggtaa aatatgcaga aaagcataag gtcaataacc gcatcgcggc
     3661 ttacatggtc gccatcgacc gtgttgcaaa agccctcaag atgcggggaa tctacgctta
     3721 acctggtaag ccgaaagaca gcgttcccct gctctggcca aggcggcaac gcaaaaaata
     3781 aatagttgca aaaaaactcc cgcggtggat aatgccgcgc gaaaggagtc cgtactttga
     3841 gcccggactc ggtgttggcc ggaaaccaga aaagcgagaa acagcaccgc cgcgatattt
     3901 gcagcgtggg gcggtggatg taccagcgcg agtacattgt ggcctgtgag ggcaacgttt
     3961 ctgtacgcct cagcgatgga cgcatcctca ccacgcctac ctgcatgaac aagggcctgc
     4021 tggaaccagc cgatctggta gttatagacc ccgacggacg tcagatcgaa gggacgcgta
     4081 gaacttcctc cgaagtgttg atgcatcttc tgttttacaa aatgcggccg gacgtgcgtg
     4141 ccatttgcca cgcccatcct gtgaccgcga caggatttgc cgccgcgggg cgcgcgctgg
     4201 atgaagccct gctccccgag gtggtcattg gcc
//
LOCUS       DASTPM010000768         5527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_575947, whole genome shotgun sequence.
ACCESSION   DASTPM010000768 DASTPM010000000
VERSION     DASTPM010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575947"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFR42_13570"
     CDS             complement(<1..838)
                     /locus_tag="VFR42_13570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEU0370241.1"
                     /translation="MPPPINNDNSPRPLVVGLGELLWDLFPKGKQLGGAPANFAYITA
                     LLGDCAVVASRVGNDRLGQEALWHLRSNRLNIDHLQRDTQHPTGTVKVEVDAKGQPEY
                     HIIENVAWDFMEFSEDWISLARSTHAVCFGSLAQRCAVSRATIKAFLSAMPSFAIGIF
                     DVNLRQSYFSSEILRDSARFAKVLKLNHEEFPRFLDLMQCPLKNSERTDVAAAHWLCK
                     EFGIRLVCVTRGSNGSLLATASSQNEHPGFRVKIADTVGAGDAFTATMVHHALRGSSL
                     SAI"
     gene            complement(852..1895)
                     /locus_tag="VFR42_13575"
     CDS             complement(852..1895)
                     /locus_tag="VFR42_13575"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0370242.1"
                     /translation="MTNTLRLRLFAGCWAISFSACGSFSFAQSAPVQPAPATNSIDQV
                     HRFNEQGKFEEALSILGDLSKTTPPPKNLAHEYGVTYYRKGDYLAAVTWLKRATEENP
                     DDREAIQLIGLSLYLAGKPNDAIPYLEKVQFWYPSANVDASYILGVAYIQTKQYPSAR
                     AAFAKMFQVPPDSAPAYLFTARLLLRFDFGPIAEEYGKKAVELDPKLPLAHQLLGELY
                     LYQSKVPEAIAQLEQELALNPGNPVGYYKLADAYSRIQKFDEAERLLQRSIWLDATST
                     GPYVLLGKALLKKGETQLAIRALPRAVSMDPSNNIPHHLLGQAYRDLGRTEDAERELK
                     LAEQLQSREDAKP"
     gene            complement(2068..>5527)
                     /locus_tag="VFR42_13580"
     CDS             complement(2068..>5527)
                     /locus_tag="VFR42_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370243.1"
                     /translation="VSADASVNVDVQLQIGEVTQSIEVTGEIPQLKTDRADVDIQFSQ
                     KYVQDLPVLNRNFTNFELLSPGTQKLPGFSHAATENPQGGGQIQVNGQHFSGTNFELD
                     GTDNQDPILGIIVVNPNLDAIAEAKVALQDYDAESGKATSGVIKVQTKSGSNEFHGSG
                     FVFYRNSDQQARDPFTNKPGVPLASATWKQFGGSVGGPIIKNKLFFFGDYQGTQQEQG
                     ITNLYTVPTSNVIKSCNPATNAQSLTPGFCDLSEYLTAFGPTAGQIFDPKTGDPLTGV
                     GRTGFTNNQIPIGRIQQNVGNVLALFPAPTAAGVNNNFVSNGTGPFDQKSFDVRIDYA
                     APHEYQVFGRFSLDYFSLSGLGGLGQLGGLGFGPGGLNGSSDVHNYSLATGFTKSIGA
                     RWLTDFRFGYFKYNPQTAYSDASASPMDTLGFPGLNSGPGIVGPPVTGGLSGFQFTLN
                     GGPTGTGNGSLSNFGDGLNIGRCNCPLTESEQQFQFVNNWTRMQGNHTIKFGADIRYA
                     MNLRVPSDASRTGLLNFDSGDTSNGGSGGLALATFLLGDVTSFQRFVSTSLDAAERQR
                     RWFFYGQDSWRVSPKFTLTYGLRWEIYFPETVNGRDKGGFANLDQGLIRVAGEGPIGL
                     NGNIDNTYKALAPRLSAAYAFDSKTVLRMGYGRGFDIGVFGSNFGHVVTQNLPVLAQQ
                     SIQDTNLNPLATNNRTAVFTLSQGPDPFSFANILGAISSNGTLPLYGPDGTVQPRIRP
                     TVQRLPTIDQWNVSLQRQITPTLNLTVAYVGNKGTHVFAGTGPSYNNNEPAIGSGTFA
                     YKCTPNPLPATTFDCKQAFVPSIPQSDRRKYFLNGVPSFSYPGFTTPTGQPLTCCAVD
                     TGYFGNDADNKYNALQIQAEKRVSHGLQFIAHYTFSHAFAYDSNYFDVSKPVAWGPNP
                     FNRNQVFVANTIYELPVGKGKRFMSNAGRAADLIIGGWQVTNTLTYGTGLPWTPSLQN
                     CGQISDAGPCRPNVLPGQNLHVGKTTAPCNASNPGAGNCTYWFTPVATMSQALPEIAN
                     SPTNITGQNDQCQTSRPTSGPFALPACGQIGDVGFNSYRGPHAFYDDMALSKSFTITE
                     RVKAQFRFDAYNVFNHPVLGVSNNFNGCVDCGGSAGQITDIEADAAPGSPVGMRQLQF
                     GFRLTF"
BASE COUNT         1175 a         1377 c         1769 g         1206 t
ORIGIN      
        1 tgatggcgga gagcgaagag ccacgcaggg cgtgatggac catggtggcg gtgaatgcgt
       61 cccccgcgcc gacggtgtcg gcgattttca cgcggaagcc gggatgctcg ttttgcgagg
      121 aagcggtggc cagcaggctg ccgttcgaac cgcgcgtgac gcacaccagc cgaatgccaa
      181 attccttgca cagccaatgc gcggcggcaa catccgtacg ctcggaattc ttcaagggac
      241 attgcatcag atcgagaaac cgcggaaatt cttcgtgatt cagcttgagg acttttgcga
      301 aacgcgcgga atcgcggaga atttcgctgc tgaaatagga ttggcgaaga ttaacatcga
      361 aaatgcctat ggcgaaggag ggcatggcgc taagaaatgc tttgatggtg gcgcgggaaa
      421 ctgcgcagcg ttgcgcgagc gagccgaaac aaacggcgtg cgtggagcgg gcgagcgaaa
      481 tccagtcctc ggaaaattcc ataaaatccc aggcgacatt ttcgatgatg tgatattccg
      541 gctgaccctt ggcgtctacc tcgactttca ccgtgccggt gggatgctgc gtgtcgcgct
      601 gaaggtgatc gatattgagg cggttggacc tcaggtgcca tagcgcctcc tgcccgaggc
      661 gatcgttgcc gacgcggctt gccacgaccg cgcagtctcc cagcagcgca gtaatgtacg
      721 cgaaatttgc gggggcgccg cccagctgct tgcctttggg aaaaaggtcc cacaaaagtt
      781 cgccgaggcc aaccacgagc gggcggggac tattgtcgtt atttataggc ggcggcatcg
      841 gtttgcgaac gtcagggctt cgcatcttcg cgggactgca gctgctcggc aagtttgagt
      901 tcgcgctcgg catcttcggt gcggccgagg tcgcgatagg cctggcccaa gaggtgatgc
      961 ggaatgttgt tactgggatc catggatacg gcgcgcggca gcgcacgaat ggccaactgc
     1021 gtttcgccct tcttgagcag ggctttcccg agaagaacgt acgggccggt ggaagtggcg
     1081 tcgagccaga tggagcgctg taagagtctt tcagcctcgt cgaatttctg gatgcgcgaa
     1141 taggcatcgg cgagcttgta gtagccgacc ggattgccgg gattcagggc gagttcctgt
     1201 tcgagctgcg cgatggcctc gggaactttg gattgataga gatagagttc gccgagaagt
     1261 tggtgcgcga gcggaagctt ggggtcgagt tctaccgctt ttttgccgta ctcttcggcg
     1321 atggggccaa aatcgaaacg cagcaacagg cgagcggtaa acagatacgc cggcgcggaa
     1381 tcaggcggaa cctggaacat ttttgcgaaa gccgcgcggg cactcggata ttgcttggtc
     1441 tggatgtaag ccactccgag aatgtaggag gcgtccacgt tggcgctcgg ataccagaac
     1501 tgtacctttt cgagataggg aatcgcgtcg tttggcttgc ctgcgagata aagagaaagg
     1561 ccgatcagct ggatggcctc gcgatcatcg ggattctctt cagtggcccg cttaagccaa
     1621 gtaacagcag cgagataatc gcccttgcgg tagtaggtaa cgccgtattc atgggcgaga
     1681 tttttcggcg gcggcgtggt tttggaaagg tcgccgagga tggataaggc ttcttcgaat
     1741 ttgccttgct cgttgaaccg atgaacttga tcgatggaat ttgtggcggg agccggttga
     1801 acgggagcgg actgagcaaa ggaaaaactg ccgcaagcgg aaaaggatat cgcccagcaa
     1861 ccagcgaaca atcgtaagcg cagggtattc gtcatgagtt ttgcatcagg ccgggaaccg
     1921 ggtctggacc aggccagctg gcgaggcgcg tggaattcac gccaccagaa ccgttggcca
     1981 ctctaccgca gtacaaattg ccgataaaca caaaaaaagg gggagtccgc gaacggactc
     2041 cccgttttgc actgcgggtg tttagggtta gaaagtcaga cggaatccaa actgaagctg
     2101 gcgcatgccg acgggagagc ccggggcggc gtctgcttcg atgtcggtaa tctggccggc
     2161 gctgccaccg caatccacgc aaccattgaa gttgttgctg acgccgagaa ctgggtggtt
     2221 gaagacgttg tacgcgtcga agcggaattg cgctttaaca cgctcggtga tggtgaagct
     2281 cttcgagagc gccatgtcgt cgtagaaggc atggggtccg cgataagaat tgaagccgac
     2341 gtcgccgatc tgaccgcaag ccggcagcgc gaagggacca gaagttggac gggaagtctg
     2401 gcactggtca ttctgaccgg taatattggt tggcgaattg gcgatttctg gtaacgcctg
     2461 gctcatggtt gcaaccggag tgaaccagta agtgcagttg ccagcgccgg ggttcgatgc
     2521 gttacatggc gccgtagtct tgccgacgtg caagttctgg ccaggcagaa cgtttggacg
     2581 gcacggtccg gcatcggaaa tctgaccgca gttctgaagg cttggtgtcc atggcaaccc
     2641 ggttccatac gtcagagtat tcgttacttg ccatccaccg ataatcagat cagcggctcg
     2701 accggcgttg ctcatgaacc gcttgccttt gccgacaggc agttcataga tcgtattggc
     2761 gacaaatact tggttgcggt tgaacgggtt cggcccccag gcgacaggct tgcttacgtc
     2821 gaagtagttc gaatcgtagg caaacgcatg ggaaaaggtg tagtgagcga taaattgcaa
     2881 gccgtggctg acgcgctttt ccgcctgaat ttgtagggcg ttgtatttgt tatccgcatc
     2941 gttgccgaag tagccggtgt caacggcgca gcaggtcagc ggctgacccg ttggcgtcgt
     3001 aaagcccgga tagctgaacg agggaacgcc gttcaggaaa tatttgcggc gatctgattg
     3061 cggtatagag ggaacgaaag cctgtttaca gtcgaacgtt gtcgcaggca gagggttggg
     3121 cgtgcactta taggcgaatg tcccgctccc gatggctggc tcattgttgt tataggaggg
     3181 accagtgccg gcgaagacgt gggtaccctt gtttcccacg taggcgacgg tcaaattgag
     3241 tgtcggcgtg atttgacgct gaagtgaaac gttccactgg tcgatggtgg gcaacctctg
     3301 cacggttggc ctgattcggg gttgaacggt gccgtccggc ccgtaaagtg gcaaggtgcc
     3361 gttggatgaa atggctccca aaatattcgc gaagctgaat gggtcgggcc cttgggataa
     3421 tgtaaagact gcagtgcggt tgttggtggc gagtggattc aagttggtgt cctgaatcga
     3481 ctgttgggca agaaccggca ggttctgcgt gactacgtgt ccgaaattgg aaccgaacac
     3541 gccgatgtca aagccgcgtc catagcccat gcggagaacg gtttttgaat caaatgcata
     3601 cgcagcactc aagcgaggag cgagagcttt gtaggtattg tcaatgttgc cgttcaggcc
     3661 tatgggccct tcgcccgcta cgcggattag gccttgatcc aaattggcga agccgccctt
     3721 gtcccggccg ttgacggttt cggggaaata gatctcccag cgcagcccgt aggtcaaagt
     3781 gaattttgga ctgacgcgcc aggaatcctg cccgtagaaa aaccaccgcc tttgtcgttc
     3841 ggctgcgtcg aggctggtgc tgacgaaccg ttggaatgaa gtcacgtctc ccagcaaaaa
     3901 ggtggcgagt gccaaaccac ccgatccgcc gttcgaagta tcgccggagt cgaagttcag
     3961 caaaccggtg cggctggcgt cggacggaac tcggaggttc atggcatagc ggatatccgc
     4021 tccgaacttg atggtgtgat tgccttgcat cctggtccaa ttgttaacga actggaactg
     4081 ctgctcgctc tcggtgagag ggcagttgca acggccgatg ttgaggccgt cgccgaagtt
     4141 cgaaaggctt ccgttaccag taccggtagg gccgccgttc aaagtgaatt ggaatccgga
     4201 aagtccgccg gtcacaggtg ggccgacaat gccgggcccc gagttgaggc cgggaaagcc
     4261 gagggtatcc atgggggatg cgctcgcgtc ggagtaggcg gtttgtgggt tgtacttgaa
     4321 gtatccgaag cggaagtccg taagccacct tgctccgata gattttgtga atccggttgc
     4381 caggctgtag ttgtgcacat cggaacttcc gttcaggccg ccgggaccaa atcccaatcc
     4441 gcccaactgg ccgagtccac ccaagccgga caaggagaaa tagtcgaggc tgaagcggcc
     4501 gaatacctgg tattcatgcg gtgcagcgta gtcaatgcgc acatcaaacg atttttgatc
     4561 gaagggaccg gttccgttgg aaacaaaatt gttgttcacg cccgctgcag taggcgctgg
     4621 gaaaagggcc agaacgttgc cgacgttttg ctggattctg ccgattggaa tctggttgtt
     4681 tgtgaaccca gtgcgcccca caccggttaa cgggtcgccc gtcttagggt cgaagatttg
     4741 tcctgcggtg ggtccaaacg ctgtgagata ttcgctaagg tcgcagaacc ccggagttag
     4801 gctttgcgcg ttagtggccg ggttgcagct cttgattaca ttgctcgtgg gaacggtata
     4861 caggttggtg atgccctgtt cctgctgggt tccctgatag tcgccgaaga agaacaactt
     4921 gttcttgatg atggggccac cgaccgagcc accgaactgt ttccaggttg cggacgcgag
     4981 aggaacgccg ggcttgtttg taaagggatc gcgcgcctgc tgatcgctgt tgcgataaaa
     5041 gacgaagccg ctgccgtgaa attcgttgga accggacttg gtctggactt taatgacgcc
     5101 cgaggtagcc ttgccggatt ccgcatcgta gtcctgcaac gcaactttgg cttctgcaat
     5161 ggcgtccaga tttgggttga cgacgatgat gccgaggatg ggatcctggt tgtcggttcc
     5221 gtccagttcg aagttggtgc cggaaaagtg ctgcccgtta acctggatct gaccaccgcc
     5281 ctgtgggttt tccgtagcgg cgtggctgaa gcccggaagc ttctgggtcc cgggcgagag
     5341 taactcaaaa ttggtgaaat tgcggtttag aaccggcagg tcctgcacat acttctggct
     5401 gaactggatg tccacgtcgg cgcggtcggt tttgagttga ggaatttcgc ccgtaacttc
     5461 gatggactga gtcacttcgc cgatctgcaa ctgcacatcc acgtttaccg aggcgtcggc
     5521 ggaaacc
//
LOCUS       DASTPM010000769         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1008422, whole genome shotgun sequence.
ACCESSION   DASTPM010000769 DASTPM010000000
VERSION     DASTPM010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008422"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFR42_13585"
     CDS             <1..186
                     /locus_tag="VFR42_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370244.1"
                     /translation="WATDSAQLRLHNVQAYGLVPFPVTEEDLKGMHAHDERIPVASFA
                     KGVSLMVRLVSEFAASR"
     gene            205..1632
                     /gene="pyk"
                     /locus_tag="VFR42_13590"
     CDS             205..1632
                     /gene="pyk"
                     /locus_tag="VFR42_13590"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU0370245.1"
                     /translation="MSFRHSKIVCTIGPASRDPRILKKLLEAGMDVARLNFSHGSHSE
                     HLATMQTLRRTAQKLKKTIAILADLQGPKIRTGPLASGTPVQLRSGQEFTITTAKILG
                     DSTRVNTTFLPLPKEVHRGDRILLSDGLIELRVEKVRGREVNCRVVNGGALGEHKGIN
                     LPGIKLRVPALTPKDKADLKFALAHGVDYIAVSFVRRSEDVQLAKSLIRRAGKDIPVI
                     AKLEKPEAIENLEEILRVSDGVMVARGDLGVEMSPERVPVVQKAIIARAREFRRPVIT
                     ATQMLESMTENPRPTRAEASDVANAIFDGSDAVMLSAETASGKYPVEAASMMARIICE
                     AEESMTEFARPATQEKLKVAETVAELVCHASRELHMRWIVVFTHSGFTARLISRYRPL
                     LPIAAFSPEAVTRRRMALVWGVVPLAIPNLRKVDQLAEISEKRLLQEKLVRKGDVIAV
                     VAGTPMGVRGTTNFMKFHIIGGAAR"
     gene            complement(1763..>2186)
                     /locus_tag="VFR42_13595"
     CDS             complement(1763..>2186)
                     /locus_tag="VFR42_13595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0370246.1"
                     /translation="ILNVIVRTVPMLWGRRAYDIPTIGASGAIFGILIACAVLFPDRR
                     AYLFPIPVAIKMKWIVAIMTAITFLGTFGLGDDKVSHICHLGGMVIGYLYLRRDSYLY
                     RLRNEVSDWKFERNKKKFEVYINKHKGEPPSRPDRWVN"
BASE COUNT          484 a          522 c          764 g          416 t
ORIGIN      
        1 tgggcgacgg actcggcgca actgcgtttg cacaatgtac aggcgtacgg attggtgccg
       61 tttcctgtga ccgaggaaga cttgaaggga atgcacgcgc atgatgagcg gattccagtc
      121 gcgtcgtttg cgaagggtgt gagcctgatg gtgcgtttgg taagcgagtt tgccgcttcg
      181 cgctgagcag aacgcaagat cctgatgagt tttcggcact ctaaaatcgt ttgtacaatc
      241 gggccggcga gccgcgaccc acgcatcctg aaaaaattgc tggaagcggg aatggacgtg
      301 gcgcggctga atttctcgca tggaagccat tccgaacatc tggccaccat gcagacactg
      361 cgccgcaccg cgcaaaaact gaagaagacc attgcgattc tggcggattt gcaagggccg
      421 aagattcgga cgggtccgct tgcgagtggc acgccggttc agttgcgttc cgggcaagaa
      481 ttcactatca ccacagcgaa aattctcggt gattcgactc gcgtaaacac cacgtttctg
      541 ccgttgccga aagaagtgca tcgcggcgac cggattctgc tgtcggatgg attgatcgaa
      601 ttgcgcgtag agaaggtacg cgggcgcgaa gtgaactgcc gggtagtgaa tggtggcgcg
      661 cttggcgagc acaagggtat caacttgccg ggaatcaagt tgcgcgttcc ggcgcttacg
      721 ccaaaggaca aggcggattt gaagtttgca ctggcgcatg gcgtggatta catcgcggtg
      781 agttttgtgc gccggtcgga agatgtgcag ctggcgaaga gcctgatccg gcgagcggga
      841 aaagatatac cggtgatcgc gaaactggag aaaccggaag cgatcgagaa tctcgaagag
      901 attttgcgcg tttcggatgg cgtgatggtg gcgcgcggcg atctgggcgt ggagatgagt
      961 ccggagcgtg tgccggtggt gcagaaagcg atcattgctc gggcgcggga atttcgccgg
     1021 ccggtgatta ccgcgacgca gatgttggag tccatgacgg aaaatccgcg ccccacacgc
     1081 gcggaagctt cggatgtggc caacgcgata tttgacggct cggacgcagt gatgctgtcg
     1141 gcggaaacgg ccagcggaaa atatccggta gaagcggcaa gcatgatggc tcggattatt
     1201 tgcgaagcgg aagaaagcat gacggagttc gcgcggccgg cgacgcagga aaaattgaag
     1261 gtggcggaaa cggtggcgga actggtatgc cacgcttcac gcgaattgca tatgcgctgg
     1321 attgtggtat tcacgcacag cggattcacg gcgcggctga tttcgcggta ccggccgctg
     1381 ctgccgattg cggcgttttc accggaagcc gtgacgcggc ggcgcatggc gctggtctgg
     1441 ggagttgtgc cgctggcgat tcccaatttg cgcaaggtgg accagctggc ggaaatttcc
     1501 gagaagcggc tgctgcagga aaagctggtg cgcaaaggcg acgtgatcgc ggtggtggcg
     1561 ggaacgccaa tgggcgtgcg cggcacgacg aacttcatga agttccacat aattggcggc
     1621 gcggcgcggt aggagcggga tagaggcaag acaggccgct gacgcaggcg aatacggcgg
     1681 atgagaaacc ttggattcgg agcgtctcga acctgtaagt caagaggcca cccgaaggtg
     1741 gccgctacga aggcgaacaa cgtcagttga cccagcggtc ggggcgggag ggaggttcgc
     1801 ctttgtgttt gttaatgtag acctcaaatt tcttcttgtt gcgctcaaac ttccagtcgg
     1861 agacttcgtt gcgcaggcgg tagagatagg agtcgcggcg gagatagagg tagccgatga
     1921 ccatgccgcc gaggtggcag atgtgggaaa ccttgtcgtc accgaggcca aatgtgccga
     1981 ggaaggtgat ggcggtcatg atggcgacga tccacttcat tttgatggcg acgggaatgg
     2041 gaaacagata agcgcggcgg tccggaaaga ggaccgcgca ggcgatcaga atgccgaaga
     2101 tagcgccgga cgcaccgatg gtgggaatgt cgtaggcacg cctgccccaa agcatcggca
     2161 cggtgcgcac gatgacgttg aggatg
//
LOCUS       DASTPM010000770         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_345233, whole genome shotgun sequence.
ACCESSION   DASTPM010000770 DASTPM010000000
VERSION     DASTPM010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345233"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFR42_13600"
     CDS             <1..535
                     /locus_tag="VFR42_13600"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEU0370247.1"
                     /translation="LGKSDGQTAVAAAQHEGFPVGTVIFLDQEEGGRMLPEQRAYLFA
                     WVDIVSAAGFRAGVYCSGIAAKEDGGVTVVTAQDIRENAAGRRIAYWVANDVCPPSPG
                     CAFPGKAPRPADSGLDFVEVWQYAQSPQRREFASGCPQNYDRDTNCYPPCIDLPRRLH
                     VDLNTATSADPSQGRTW"
     gene            694..1872
                     /locus_tag="VFR42_13605"
     CDS             694..1872
                     /locus_tag="VFR42_13605"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU0370248.1"
                     /translation="MRDVVIASAVRTPVGRAFKGTLRATRPDELAAIAIQGALERVSQ
                     VDPKEIEDVILGCATPEAEQGSNVARIASLRAGLPLEVSAITINRFCSSGLQSIAMAA
                     ERIMSGGAEIIVAGGTESMSMIPMGGHKISPNPWLVEHYPDSYLTMGLTAERVAQRLG
                     ITRPAADEFSLRSHRNALAAIQAGKFEEETVPVPVSFTTPNGSRPKRQEIVFKVDEGP
                     RADTSIEALQALKPVFHAKGTVTAGNSSQTSDGAAAAVVMSAERAQALGIRPLVRYVS
                     FATAGYKPEEMGLGPVYAIPKALRLAGLKLSDIDVIELNEAFAVQALGVIKEAGLDLE
                     RVNPNGGAIALGHPLGCTGAKLTASIIRELKRRGSRYGMVTMCVGGGMGAAGIFENLS
                     "
     gene            2020..>2851
                     /locus_tag="VFR42_13610"
     CDS             2020..>2851
                     /locus_tag="VFR42_13610"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0370249.1"
                     /translation="MATTAIPSTRIPGGSFLLEPRKPDEVFTPEDFTEQHQLIGQTAE
                     EFAVNEILPNADKIEHKDFSVSRDLLRKAGDLGLSGVEIPEAYGGLEMDKVTAAVIAD
                     HIAKYAGFATTWGAHSGIGMLPIVYFGTEEQKKKYLPKLAAGEIVGAYALSEASSGSD
                     ALHCRARAQLSPDGKNYILNGEKMWITNAGFADLFTVFAKVDGEKFTAFLIEKNFPGF
                     SVGGEEHKMGIRGSSTCPLILNECKVPAENVLGEIGKGHVIAFNILNVGRFKLGAMCV
                     G"
BASE COUNT          601 a          803 c          877 g          570 t
ORIGIN      
        1 gttaggcaaa tccgacggcc agacggcagt tgcagcggca caacatgaag gctttcctgt
       61 gggcacagta atcttcctcg atcaggaaga aggcggccgg atgctgccgg aacagagggc
      121 ctacctattt gcctgggtag atatagtaag tgccgcgggc ttccgcgccg gtgtttactg
      181 ctctggcatc gccgccaaag aggacggcgg cgtgacggtg gttactgccc aggacatccg
      241 tgaaaatgca gccggaagaa gaatcgcgta ttgggtagca aacgacgtct gcccgccttc
      301 gccaggatgc gcttttccag ggaaggcacc gaggcccgcc gacagcggct tggacttcgt
      361 ggaagtgtgg caatacgctc aatcgccgca gcggcgggag ttcgcctccg gatgtccaca
      421 aaattatgat cgtgacacaa attgttatcc accctgcatt gatctcccca ggcggttgca
      481 cgtggatctg aatacggcga cgagtgccga cccgtcgcag ggaagaacct ggtgatgatg
      541 gcactggcct gcagggtggc cttttcctgg caatgtttcc gtcaagatgc caatgggagg
      601 cgtaaagcat ctaatcttcg gttgccctag agtgtgggtc tggcacgcct gcccggtgct
      661 agacttgttt tcatcagccg cgggaaggtt cctatgagag acgtggtcat tgcttccgct
      721 gtccgtaccc ccgtgggacg cgccttcaaa ggtacgctcc gcgccacccg ccccgatgaa
      781 cttgcagcca tcgccatcca gggcgcactg gagcgggtct cgcaggttga tcccaaagaa
      841 atcgaagatg taattctggg ctgtgccacg cccgaggccg agcaaggttc gaacgtggct
      901 cgcatcgcat cgttgcgcgc cggactgccg ctcgaagttt ccgcgatcac cattaaccgc
      961 ttctgctcct ccggactgca gtcgatcgca atggcggcgg aacggatcat gagtggcggg
     1021 gcggagatca tcgtcgccgg cggaactgaa tcaatgtcca tgatcccgat gggggggcat
     1081 aaaatttcac ccaacccgtg gctggtggaa cattatcccg attcctacct caccatgggc
     1141 ctcaccgccg agcgggtggc tcaacgcttg ggcatcacgc gcccggcagc ggatgaattc
     1201 tcgcttcgca gccatcgcaa tgcattggcg gcgattcagg cgggcaagtt cgaagaggaa
     1261 acggtgccgg taccggtcag tttcacgact ccgaacggct cgagaccgaa gcgacaggaa
     1321 atcgtcttca aagtggacga aggcccgcgc gccgacacct cgattgaggc tctgcaggcg
     1381 ctgaagccgg tctttcatgc caagggaacg gtgactgcag gcaattcgtc gcagacttcc
     1441 gacggtgcgg ccgccgccgt ggtcatgtcc gcggagcgcg cccaggcttt gggcattcga
     1501 ccgcttgtgc gctacgtctc tttcgctacg gcaggatata agccagagga gatggggcta
     1561 ggcccggtgt atgcgattcc caaggcgctg cggcttgcgg gacttaagct ttccgacatt
     1621 gacgtgatcg agttgaacga agccttcgcg gtacaggcgc tgggcgtaat caaggaagcc
     1681 ggcctcgatc tggagcgagt gaacccgaac ggtggagcca ttgcgctcgg acatcctctg
     1741 ggttgtaccg gagccaagct gactgcctcg atcattcgcg aactgaagcg gcgcggatcg
     1801 cgctacggga tggttaccat gtgcgtgggc gggggcatgg gtgcggccgg gatattcgaa
     1861 aacctgagct aacgtcggaa acacggagtc acagaacaga caagggattt tgaatctccc
     1921 gttatcccct ccgccagttg acattaaagt acaatggacc cagcggtttt ctccgtttct
     1981 ccaaggctcc gtggtggaat tggctttcaa ggagtgaaga tggccacgac tgcaattcct
     2041 agtaccagga tccccggcgg cagctttctt ctcgaaccgc gcaagcctga cgaagtgttc
     2101 actcccgagg attttaccga gcaacaccaa ctcatcgggc agacggccga ggagtttgcc
     2161 gttaacgaga ttctccccaa cgccgacaaa atcgagcaca aggacttttc tgtcagtcgc
     2221 gatctgcttc gaaaggccgg ggacctgggt ctgagcgggg tggagattcc ggaagcctac
     2281 ggcggcctgg agatggacaa agtcacggcg gccgtgattg ccgaccacat cgccaaatat
     2341 gccggctttg ccaccacctg gggagcgcac agcggcattg gaatgttgcc catcgtgtat
     2401 ttcggtacag aggagcaaaa aaagaagtac ctacccaagc tggcggcggg agagattgtc
     2461 ggcgcctatg ccctgtcgga agcttcgtcg gggtcggacg cgttgcattg ccgagcccga
     2521 gcccagctct cgcccgacgg caagaactac atcctgaacg gcgagaagat gtggatcacc
     2581 aacgccgggt ttgcagacct atttaccgtg ttcgccaaag ttgatggcga aaaattcact
     2641 gcgtttctga tcgaaaagaa ctttcctgga ttctccgtcg gcggggaaga acataagatg
     2701 ggcattcgcg gctcttccac ctgccccttg atcctcaatg agtgcaaggt tcccgcggaa
     2761 aatgtgctgg gcgagatcgg caaagggcac gttatcgcct tcaacatcct gaacgtcggg
     2821 cgcttcaagt tgggcgcgat gtgcgtaggc g
//
LOCUS       DASTPM010000771         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_776751, whole genome shotgun sequence.
ACCESSION   DASTPM010000771 DASTPM010000000
VERSION     DASTPM010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776751"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..982)
                     /locus_tag="VFR42_13615"
     CDS             complement(116..982)
                     /locus_tag="VFR42_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0370250.1"
                     /translation="MEKTIMERRQFLGGAIGTGVALAAGKWNTAAARGSLQVGLELYS
                     MRKELDRDVPQALSLVRRMGFAEVEVAMLHGLSANEFAAALKAAGLKATSMAAGYEDL
                     QAKLDLVKQRAEEFLAKWVVLAWIPHSGDFTVETAKRVAREMNEWGESLGKAGRRLAY
                     HPHGYEFRPEANGSAFDALLKETNPKTVFFEVDTFWAEMAGQNCAQLITRLGSRVRLL
                     HLKDLRRGVQTAFTGAAADEDSVALGDGVIDWRGVLAAARRAGVERYFIEDDSPVVEK
                     QVPRSLEFLAAM"
     gene            complement(1114..2289)
                     /locus_tag="VFR42_13620"
     CDS             complement(1114..2289)
                     /locus_tag="VFR42_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370251.1"
                     /translation="MVKRLLHITLLSLLIGAGVWAQQAGTVVGASPGSAKRVGGIKVE
                     RVVPPKPTCESAGLAMGTEAEGGLPEMEFWRQDLLCVRHSDGKTEAVRDGLPWGAGVV
                     SAQGDVAYWIAEKSELHVFSPKTNVDTVMDTLPGANLRQMVWSLKGRTLVYFAVKADP
                     AGIRVVNLDSGARNLISGNFVSVVASPDPEYLATVGWEGVQRVHVADGRREDVAKIEL
                     AADAAYSQGGKWLGVEATPASDPTLTANGPNQAASEDDSPDCTGGAFALTVFETATKR
                     AVSVPFPKGFDTVLDFEFSPDERALAVTYGVTGCDYPGDAARVFVVSLADLKMTPISP
                     ENKLSVEAHWSPGGKKIVFLDYTGSDAGIVIADVKTGNVVRVTSPGQNGPDKWVAWR"
BASE COUNT          556 a         1064 c          616 g          581 t
ORIGIN      
        1 ggcttgcgtt gttcaggggc cggagcttta gttccgactt ctgaccgtcc gtagatgggc
       61 tgcagcccct gctggaagtt cttacttcat cgttcgcccc tggcgtcgcc caatcttaca
      121 tcgccgccaa aaactccaaa cttctcggca cctgtttttc caccaccgga ctatcatcct
      181 caataaagta tcgttccact cccgcccgcc gcgccgccgc cagaactccg cgccaatcaa
      241 tcacgccgtc acccagcgcc acgctgtctt cgtccgcggc cgctcccgtg aatgccgtct
      301 gcactcccct gcgcaggtcc ttcaaatgca aaagccgcac ccggctcccc aatcgcgtga
      361 tcagctgtgc gcaattctgt cccgccattt ccgcccaaaa tgtatccacc tcaaaaaaca
      421 ctgtcttggg gttagtctcc ttgagcagcg catcaaacgc gcttccattc gcttcgggac
      481 ggaactcata accatgcgga tgatacgcca gccgtcttcc ggctttcccc agcgactccc
      541 cccactcatt catctctcgc gccacgcgct ttgcggtctc caccgtaaaa tccccgctgt
      601 gcggaatcca cgccaggacc acccacttcg ctaaaaactc ctccgctctc tgtttcacca
      661 ggtccagctt cgcttgcaaa tcctcatacc ccgcggccat cgaagtcgcc ttcaatcccg
      721 ccgccttcag cgccgccgcg aactcattcg cgctcaaacc atgcagcatc gcaacctcca
      781 cttccgcgaa ccccatcctc cgcaccagcg acagcgcctg cggaacatcc cgatccagct
      841 ccttcctcat gctgtagagc tccagcccca cctgcaacga accgcgcgca gccgccgtgt
      901 tccatttccc ggccgcaagc gcaactccgg tcccgatcgc cccgcccaaa aactgccgcc
      961 gctccattat cgttttctcc atccctacca cagtcgttcg gggaggcgga gcttcagctc
     1021 cgacataagt gcttggctat caaactggct tcagcccctg aggagttgtt ttttccggtt
     1081 atacaggagg ccttcggcgc ggcgctcttc acctcaccgc cacgccaccc acttatccgg
     1141 cccgttctgc cccggacttg taactcgcac cacgttcccc gtcttcacgt ccgcaatcac
     1201 aatccctgca tcgctcccgg tgtaatccaa aaacacaatc ttcttccctc ccggcgacca
     1261 atgcgcttcg acgctcagtt tgttctctgg cgaaatgggc gtcatcttca gatcggccag
     1321 cgaaactaca aacacgcgcg ccgcatcgcc cggatagtcg catccggtca ctccatacgt
     1381 caccgccagc gcgcgttcat ccggcgaaaa ctcaaaatcc agcaccgtgt cgaaaccttt
     1441 tggaaacggc acactcactg ctcgcttggt cgctgtctca aacaccgtca gcgcgaaggc
     1501 gccgcccgta caatccggcg aatcgtcctc actcgcggcc tggtttggcc cattcgccgt
     1561 cagggtcgga tccgaagcag gcgtcgcttc cacgcctagc cattttccgc cctgcgaata
     1621 cgccgcgtca gctgccaatt ctattttcgc gacatcttct cgccggccat cggcaacgtg
     1681 aacccgttgc acgccttccc accccaccgt cgccaaatat tccggatcgg gcgacgccac
     1741 cacactcaca aaatttcccg aaatcagatt tcgcgctcca gagtccaggt tcaccacgcg
     1801 aatacccgcc ggatccgcct tcaccgcaaa ataaaccagc gtccgcccct tcaatgacca
     1861 caccatctgc cgcaaatttg ctcccggcag cgtgtccatc accgtgtcca catttgtctt
     1921 cggcgaaaac acgtgcagtt cgctcttctc cgcaatccag taggcgacat cgccctgcgc
     1981 ggaaaccaca cccgctcccc atggcagccc gtcgcgcacc gcttccgtct tgccgtcgct
     2041 gtgccgcacg cacaataagt cctgtcgcca gaattccatc tcgggcaatc cgccttccgc
     2101 ctccgttccc atcgcaagtc ccgccgactc acacgtcggc ttcggcggca ccactcgctc
     2161 taccttaatc ccgccaactc tcttcgccga acccggactc gccccaacca ccgtccccgc
     2221 ctgctgcgcc cacacacccg caccaatgag caaagaaagc agagtgatgt gcaacagcct
     2281 tttcaccata cccctttcgc cactctaggt agttctgccg ccagtatctc ctccctctcg
     2341 ttccccaagg agctgcccgg cgcgaatcag tcacctgctc cccagaccag ccgccgctcc
     2401 agaacaccgg ccgccccgtc tccatccttc tgcgccgtgc cctatgaatc gccgactggc
     2461 cgtcacttct cccgtcaact tcgcactacg cggtcctgtt gaattgggaa ccgttcgatt
     2521 gaatcgaggc agattgagta atttcgcaag cgttgcgacc gcctctctgg tggacgaagt
     2581 cccaatcgag tcatcgacgg ggaggtgtcc ttttctaatc gaaacgcacg ctagcgacgc
     2641 aaggttatgc ggaattggct caactgagtc ccatcccaaa gaagtcccgg tgtgtccgaa
     2701 taagtcaacg accgcaatag cgtagcgccg ttcgattgcc acgcttagga aaaagtacgg
     2761 agtgtaggag agtcaaatac gaggcccgcg tgttcaggaa cgcgcaaagt ggcgttc
//
LOCUS       DASTPM010000772         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_921856, whole genome shotgun sequence.
ACCESSION   DASTPM010000772 DASTPM010000000
VERSION     DASTPM010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921856"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VFR42_13625"
     CDS             complement(<1..1493)
                     /locus_tag="VFR42_13625"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU0370252.1"
                     /translation="MKASIQRRLTVIFGSVFLIGIGLVLASCSGNGSAPSTPASSPAV
                     PLSAMEVDTVVHNAALSVNTPLVIAVTDRSGAILAVFQTPGAPATSAGNFGVAVDSNE
                     LAVSLARSAALFSNDQAPLSSRTVRFISGIHFPPGVAFAPNADLFGIENTNRGCPLSA
                     SFNPGKAINPARSIDGLDLGLGVTTGKADVMDSNPAAVNPGGVPLFKNGHVVGGIGVV
                     SSALDAAEFAAFSGASQSGFLPTVAQLPAPGVVIIGGIALPFVNQTSAPPGIAAGTGA
                     GAYVVGPSVGGLPPEGDLVGPNGSSSGLTQSDVAAIISQAISTAELTRAVIRLPSGSR
                     TRMTIAVTDLDGTVLALYRMPDATVFSIDVAVAKARNVIYFSSAGAADLPGLPSGTSV
                     TNRTIGFGAQPLFPPGIDGSNSGPFFNLYTQDIANPCTQGSQAPNPNQSGIVFFPGSL
                     PLYKNGVLVGGLGVSGDGVDQDDYVSNAAAQGFEAPTNIRADQVMID"
     gene            1719..>2404
                     /locus_tag="VFR42_13630"
     CDS             1719..>2404
                     /locus_tag="VFR42_13630"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEU0370253.1"
                     /translation="MNTCKRTVAAVLCLLLSPLPAVCQSASGDHAGQINALVPDATRN
                     AKPAKAKDDLNWNDLLQTQKSGRLRAGLKDGSILSLGSNSELRVVQHDAATQQTSLEV
                     DFGKVRSKVVKITQPNGKFEIKTPNAVIGVVGTDFFVGYEADRTTVICYRGKVMVTPS
                     GNAKILKNSGQADGSNSIAVAEGQMVIISSEIPAGGFQSSAAPKDLLQASMNDTTVPD
                     GPVASVHPHLV"
BASE COUNT          520 a          689 c          732 g          463 t
ORIGIN      
        1 ctatcgatca tgacctgatc tgcccgaatg tttgtgggag cttcaaagcc ctgggctgcg
       61 gcattggaca catagtcatc ctgatcgacg ccatcaccac tgacgccgag tccgccgacc
      121 agaactccgt tcttgtagag cggaaggctt ccgggaaaga agacgatgcc actttgattc
      181 ggattcgggg cctgcgagcc ctgcgtgcaa gggttggcta tatcctgggt atagagattg
      241 aagaaaggtc cggagttcga gccgtcgatc cctggaggga acaatggctg cgctccgaag
      301 ccaatcgtcc ggttggtaac cgacgttcct gacgggaggc cgggcaagtc ggctgcgccc
      361 gccgagctga agtaaatcac attgcgcgcc ttggcgacgg ctacatcgat gctaaacact
      421 gttgcgtcgg gcatgcggta gagggcgaga acagtgccat cgaggtctgt gacggcgatc
      481 gtcatgcgcg tgcgggatcc cgagggcagg cggatcacag cgcgggtcaa ttccgcggtc
      541 gaaattgcct gcgaaataat ggccgccacg tctgactgcg tgaggccact ggaactacca
      601 ttcggaccaa ccaggtctcc ttccggggga agtcctccga cgctcggccc cacaacgtag
      661 gcgccagcgc ccgtgcccgc agcgatgccc ggaggcgcgc tcgtctggtt cacgaacggc
      721 aacgcgatgc cgccgatgat cacgacaccg ggcgcgggca attgagccac agttggcaga
      781 aacccggatt gcgatgctcc cgagaacgcc gcaaattctg ccgcgtcaag cgcactgctc
      841 actacgccaa ttcctcccac cacgtgcccg ttcttgaaga ggggaacgcc accggggttc
      901 acggcggccg gattactgtc catcacgtcc gctttgccgg tcgtgacccc aagtccgaga
      961 tctaggccgt cgatcgaacg cgccggattg atcgctttgc ccggattgaa actggcactg
     1021 agggggcagc cgcggttggt gttctcaatt ccaaacaggt cggcatttgg ggcgaacgca
     1081 actccgggag ggaagtggat tccgctgatg aagcgcaccg tcctgcttga aagcggagcc
     1141 tggtcgttgc taaagagggc ggcgcttcgc gcgagagaga ccgcgagttc gttgctatcg
     1201 accgccacac cgaaattgcc ggccgacgtg gccggcgccc ctggggtctg aaagacggcg
     1261 agaattgctc ctgagcggtc agtcacagca atcactaaag gcgtgtttac ggacagggcc
     1321 gcgttgtgga caaccgtatc cacctccatt gcagagagtg ggaccgcagg ggatgacgct
     1381 ggcgtcgagg gagcgctacc attgccgctg caggaagcca ggacgaggcc aatgccgata
     1441 aggaacaccg aaccgaaaat gaccgtcaat cgtcgctgaa tggaggcctt catgttatgc
     1501 ggaaacagag gcggagcaaa aaacttgcct ggtgtctccg ctgagaactt atcccaagag
     1561 cgaatgtaat atgccgcgcc cccgagaacg acagtgttgc gaccttcgtg taaccacagt
     1621 gatcactgtg gcaaggtggt aacgttgaca ttcctcctca ctctgtcaga atttgctcag
     1681 tcctccatat tgttttaaag aactggggag atctcacttt gaatacctgc aaacgaacgg
     1741 ttgcggccgt cctctgcctg ctgctttcac ccttgcctgc cgtttgtcag tcggcttcgg
     1801 gcgatcatgc tggtcaaatt aatgcgcttg ttccggatgc gacccgcaac gcgaagcctg
     1861 caaaagcgaa agatgacctc aattggaacg accttctgca aacccagaag tcaggccgcc
     1921 tgcgcgccgg gttgaaggat ggttccattc tcagcctggg ctcgaacagc gaattgcgcg
     1981 tcgtgcagca cgatgcagcc acgcagcaga cttcgctgga agtggatttc ggcaaagtcc
     2041 gcagcaaagt cgtgaagatc actcaaccaa atggcaagtt tgagattaag acgcccaacg
     2101 cggtgatcgg tgtggtgggc acggatttct ttgtgggcta tgaagccgac cgcaccacgg
     2161 tgatctgcta tcgcggcaag gtgatggtga cgccctccgg caacgccaag atcttgaaaa
     2221 attccggaca ggcggatggc agcaattcca tcgccgtggc tgaagggcag atggtgatca
     2281 tcagctccga gattccggcc ggcggatttc aatccagtgc agcgccgaag gatctgcttc
     2341 aggccagcat gaacgatacg acggtgcccg atggcccggt ggcctcggtc cacccgcatc
     2401 tggt
//
LOCUS       DASTPM010000773         8085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_835211, whole genome shotgun sequence.
ACCESSION   DASTPM010000773 DASTPM010000000
VERSION     DASTPM010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835211"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..370
                     /locus_tag="VFR42_13635"
     CDS             92..370
                     /locus_tag="VFR42_13635"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEU0370254.1"
                     /translation="MSKLAKAFTAKLQKSPNRGGWTYVVWPQSVKFFGTRGLVKVKGK
                     IEGHKFQSSFMALGDGRHKLPVKAEIQKAIGKKTGQTVRVVLEEGIER"
     gene            424..2826
                     /gene="purL"
                     /locus_tag="VFR42_13640"
     CDS             424..2826
                     /gene="purL"
                     /locus_tag="VFR42_13640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU0370255.1"
                     /translation="MATAATEIKVTSEIAAEHGVTREEYARIQKILGRDPNITELGIF
                     SVMWSEHCSYKSSKVHLKRLPTRGKLVVQGPGENAGVVDIGDGLVAAFKIESHNHPSY
                     VEPFQGATTGVGGILRDIFTMGARPIAVLDSLRFGPITENAHVAPGFSLASSDSSAVA
                     ANRRILDGVIRGIAHYGNCFGVPTIGGEVCFEPCYSQNPLVNALALGIARKEEIFLAK
                     AKGVGNPVIYVGAKTGRDGIHGASLLASAEFTEESQQKRPNVQVGDPFVEKLLLEACL
                     EAMRTGAVVAIQDMGAAGLTCSTMEMASRGGTGIEIDLAKVPQRETGMTPYEIMLSES
                     QERMLLVAERGRENEVLDIFKKWGLDAVVIGAVKGGELAVVKNHGNVAAEIPAHPLAE
                     EGPVYQRPIASPAPRVESEKDWFRFAPDETYLTENFEKLLASPAIASKRWITEQYDTS
                     VRTNTLAGPGASDAAVVRIKESEKNGPNGTIMKALALATDGNGRWCQLNPKLGAMHAV
                     AEAARNVAASGARPIAATNCLNFGSPERPEVMWQFSQAIDGIGEACAALGTPITGGNV
                     SFYNETLGKSIYPTPVIGVLGVLDDASHALKIAFRNAGDIIVLLDASVVGAQHVAPQL
                     GKQSSVSQAREFSSSEYSKTIAAIVAGEPPAIDLAAEKRLIDCLVAIASESHVTSAHD
                     ISEGGLAVTLAESCFSSVGAQHVAPQLGAMVRIRDGVSSESSLFNERGARCIVSLPPE
                     KIGALQLIARQYGVAADQIGQVTSTGSLRIEYKGYAVIDSAVEKLRDIWANSLERTLL
                     SK"
     gene            2823..4295
                     /gene="purF"
                     /locus_tag="VFR42_13645"
     CDS             2823..4295
                     /gene="purF"
                     /locus_tag="VFR42_13645"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU0370256.1"
                     /translation="MTQKVAYADDHFHDHCGVVGVYGHEEAAKLVYLGLYALQHRGQE
                     SAGIVSTDGRELHAHRAMGEVEEIFKPDVLNKLPGTASIGHTRYSTAGDKALLNAQPI
                     MIDCNKGKVSIGHNGNLTNAAEWRRKLEHKGSIFQTNSDTEVIVHLLARSQARHLSGA
                     LGDALNQVEGAYSLVVLTTDELYAIRDPRGFRPLNLGKLVAPNGEVAWIVASETCAFD
                     LLNAQYIREIEPGEMLRISRSGLESIHFAPEKPHQFCIFEHVYFSRPDSTIFGRSVND
                     SREMLGRLLAREHPVDADIVVPVPDSGVPAAVGYAMESRIPFRMGLIRNHYIGRTFIE
                     PSQAIRNFGVKLKLNPIRGLMEGKRVILVDDSIVRGTTSRKIVRMVREAGAKEVHVRI
                     SCPPTISPCYYGVDTPTREELIASCNTPEEICRFLGADSLGYISLTGLRQAVNDTKGN
                     FCTSCYTGNYPTDLVQLEVREGANLGKLTDSAPVMVERAP"
     gene            4324..5067
                     /gene="lepB"
                     /locus_tag="VFR42_13650"
     CDS             4324..5067
                     /gene="lepB"
                     /locus_tag="VFR42_13650"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0370257.1"
                     /translation="MKYKKEQSKKPPDKRKQPELTMEQKVWREVVAWFWVGLVFLLIN
                     GAVGQARVIPSESMQNTLLVGDHLIMSRMGYDAGIPFTNVHVPLWRNPKRGQIVIFRP
                     VIPGETSDVIKRVIGLPGETVDVRDGSVWINGTRLQEPYTVGRTEPGDPGMFRGALAG
                     QNNVLPYKIPPDCYFVMGDNRGNSLDSRFVGCIPRANIVGTPVMIYMSIDTPSESWGS
                     GAITDRVSAYANALIHPSEVRWRRLFRLF"
     gene            5106..5831
                     /gene="lepB"
                     /locus_tag="VFR42_13655"
     CDS             5106..5831
                     /gene="lepB"
                     /locus_tag="VFR42_13655"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0370258.1"
                     /translation="MKAEKVQSKPQDKLTMEQKVRREIIAWFWVGLVFLLINGAIGQA
                     RVIPSGSMENTLLIGDHLIMSRVGYDAGIPFTGWHVPLWRNPKRQQIIIFKPPFDLSQ
                     PDYVKRAIGLPGDIIDIHDGAVWINGERLAETYTLGKTERVDPGLYAGAFAGMPQVMP
                     YKIPDNCYFAMGDNRQNSFDSRFWGCVPRKDIIGTPVLIYMSLNAGTEPWQPGKIGER
                     FLAYLNGIIHPGTVRWRRLFHVF"
     gene            5932..6978
                     /gene="purM"
                     /locus_tag="VFR42_13660"
     CDS             5932..6978
                     /gene="purM"
                     /locus_tag="VFR42_13660"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU0370259.1"
                     /translation="MRYADAGVNIAVADEAKSRIRHLATRTFTNGVLGGIGSFGALFA
                     LDMKKFKEPILVSSADGVGTKLKVAIAMNVHSTVGGDLVNHCVNDILVQGAEPLFFLD
                     YLALGKMDPRVVEQLVDGMSRACRKAGCALIGGETAEMPGFYAAGDYDLAGFIVGAVE
                     KKNLLTGKNVKPGDTLLALPSAGLHTNGYSLARKLAFEVAKLKPETYVAEVGNKIGAE
                     LLKPHLCYAPALRTILANGWVSALAHITGGGIPGNLPRVLPAGVKAQIDLASWPVLPI
                     FKYLAKVGKMDSDALLESFNLGVGMVLVVPSAYVKKVEADLKRRREKFFQIGRIERGD
                     TAKARVVYSGALSL"
     gene            complement(7099..>8085)
                     /locus_tag="VFR42_13665"
     CDS             complement(7099..>8085)
                     /locus_tag="VFR42_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU0370260.1"
                     /translation="YALIRLGFVDETEGFIDWLKGRLGDDAQRGPLQVMYGIDGRQDL
                     EEISLDHLFGYENSRPVRIGNAAFKQLQLDIYGEMMDAIYLSNKYGRPISHQGWQEVQ
                     RILEWLGNNWRRPDEGIWEVRGGAREFLHSRVMCWVAFDRGLRLAHKRSLSGPLDVWQ
                     RIRDEIRSDIFTNFWNADLQSFVQVKGTKDLDASALLMPMMRFISPVDPMWLSTLRAI
                     EKHLVEDTLVRRYQVEQSHVDGLPGSEGSFTACSFWYVECLARAGELEKAQLMFEKLL
                     GYANHLGLYSEELGVNGRHLGNFPQAFTHLALISAATYLDRALSGTNESVWR"
BASE COUNT         1777 a         2397 c         2316 g         1595 t
ORIGIN      
        1 cgaatccgcg ctcgactccg ccgacggcct ggtgttgttc cattccatgg tcgaatcgct
       61 agtcggcgca gccagggcca ctgcttgaaa catgtcgaaa ctggcaaaag ccttcacggc
      121 caaactgcag aagagcccca atcgcggcgg ctggacgtat gtcgtttggc cccagtcggt
      181 gaaatttttt ggcacccgtg gtttggtaaa agtaaaggga aaaatcgaag gccataaatt
      241 ccaaagctcc ttcatggctt taggcgacgg caggcacaag ctgcccgtga aagccgagat
      301 ccagaaagcg atcggcaaaa aaacgggtca aactgtaagg gtcgtcctgg aagaaggaat
      361 cgagcggtga gtttataccg cagcaagttg catcgcggga agcggtagaa ggcgaagagg
      421 ttcatggcca cggcagcaac agaaatcaaa gtcacatcgg agatcgcggc ggagcacggc
      481 gtaacccgcg aggaatacgc gcgcatccag aaaattctgg gccgcgatcc caacatcacc
      541 gaactcggca ttttttccgt gatgtggagc gagcactgct cctacaagtc cagcaaagtc
      601 catctgaagc gtctgcccac cagaggcaaa ctggtcgtgc aaggccccgg cgaaaatgcc
      661 ggtgtggtag acatcggcga cggcctggtc gccgcgttca aaatcgaatc gcacaatcat
      721 cccagttacg tcgagccgtt tcagggagcc accaccggcg tcggcggaat cctgcgcgac
      781 atcttcacca tgggcgcccg ccccatcgcg gtcctcgact ctcttcgctt cggcccgatt
      841 actgagaatg cccatgtagc gccaggcttt agcctggcat cttctgactc ctcggcggtg
      901 gccgccaatc gccgcatcct cgacggcgta atccgcggca tcgcccacta cggcaactgt
      961 tttggcgttc ccacgatcgg cggcgaagtc tgtttcgagc cgtgctattc gcagaatccg
     1021 ctggtcaacg cgctggcgct cggcatcgcg cgcaaggaag aaatttttct cgccaaagcc
     1081 aaaggcgtcg gcaatccggt catttacgtc ggcgcaaaaa ccggtcgcga cggcatccat
     1141 ggtgcttcgc tcctcgcttc cgccgagttt accgaagaat cccagcaaaa acgtcccaac
     1201 gtgcaagtcg gcgatccctt cgtggaaaag ctgctgctcg aagcctgcct cgaagccatg
     1261 cgcaccggcg cagtcgttgc cattcaggac atgggcgccg ccggtctgac ctgctccacg
     1321 atggaaatgg cgtcccgcgg cggcacgggc attgaaattg acctcgccaa agtcccgcag
     1381 cgcgaaaccg gcatgacgcc gtacgaaatc atgctcagtg aatcacaaga gcgcatgctg
     1441 ctggtcgcgg aacgcggccg cgaaaatgaa gtcctcgaca ttttcaagaa gtggggcctc
     1501 gacgccgtcg tcatcggcgc agtaaaaggc ggcgaactcg ccgtagtgaa aaatcacggc
     1561 aatgtcgcgg cggaaattcc cgcgcacccg ctcgctgaag agggacccgt ctaccagcgg
     1621 cctattgcga gtccagcacc acgagtcgaa tctgaaaaag attggttccg tttcgcgccc
     1681 gacgaaacct acctcaccga aaatttcgag aaactgctgg cctcgccggc gatcgcttcg
     1741 aagcgctgga tcaccgagca gtacgacacc tcggtgcgaa ccaacaccct cgcaggccct
     1801 ggcgccagcg atgccgccgt cgttcgaatc aaggaaagtg aaaagaacgg cccaaatgga
     1861 acaataatga aagcgctggc cctcgccacc gacggcaacg gacgctggtg ccagctgaat
     1921 ccgaaactgg gagccatgca cgccgtagcg gaagcggctc gcaacgtcgc cgccagcggc
     1981 gcgcgcccca tcgccgcaac caattgcctc aatttcggca gccccgaaag gcctgaagta
     2041 atgtggcaat tttcccaggc catcgacggc atcggtgaag cctgcgccgc cctcggcacg
     2101 ccaatcaccg gcggcaacgt cagcttttac aacgaaactc tcggcaaatc catctacccc
     2161 acgcccgtaa tcggcgtcct cggagtcctc gacgacgctt cccatgccct caaaattgcg
     2221 ttccgcaacg ccggcgacat catcgtcctc ctcgacgctt ctgtcgttgg ggcgcaacat
     2281 gttgcgccgc agctcggcaa gcaatcgtcc gtatcgcaag cacgagaatt ttcttcctca
     2341 gaatattcaa agaccatcgc cgccatcgta gccggcgaac cgcccgccat cgatctcgct
     2401 gccgaaaaac gactgatcga ttgcctcgta gcgatcgcgt cggagtcaca tgtgacatcg
     2461 gcccacgaca tctcagaggg cggtttagcc gtgacactcg ccgagtcctg tttctcctcc
     2521 gtaggggcgc aacacgttgc gccccagctt ggcgcgatgg tgcgcatcag ggatggcgtg
     2581 tcttccgaat cctcgctctt caacgaacgc ggcgcccgct gcatcgtcag ccttccgccc
     2641 gaaaagattg gcgccctgca attgattgcg cgacaatacg gcgtcgccgc ggaccaaatc
     2701 ggccaggtta cctcaaccgg ctcgctccgc atcgaatata aggggtacgc agtgatcgat
     2761 tctgcggtcg agaaattgcg cgacatctgg gccaactcgc tcgaacgcac gctgctatcg
     2821 aaatgacgca aaaagtcgca tatgcggacg atcacttcca cgaccattgt ggcgtggtag
     2881 gagtgtacgg ccacgaagaa gccgcgaaac tcgtgtatct cggcctgtat gcgctgcagc
     2941 atcgcggcca ggaatccgcc ggcatcgttt cgaccgacgg tcgcgaactc cacgctcatc
     3001 gcgccatggg ggaagtcgaa gaaatcttca agccggatgt cctgaacaag ttgccgggaa
     3061 ccgcttccat cggccacacc cgctattcca cggcgggcga taaagcgctt ctcaacgctc
     3121 agcccatcat gatcgattgc aacaagggca aagtgtccat cggccacaac ggaaatctca
     3181 ccaacgccgc cgagtggcgc cgcaaactcg aacacaaagg ctcaattttc cagaccaaca
     3241 gtgacaccga agtcatcgtg catttgctgg cacgttcgca ggcgcggcat ctttcaggag
     3301 cgttaggcga cgcgctcaat caagtcgaag gcgcctattc gctcgtcgtc ctcaccaccg
     3361 acgaactcta cgctatccgc gatccgcgcg gcttccgtcc tttgaatctt ggcaagctgg
     3421 tcgctccgaa tggcgaggtc gcgtggatcg tcgccagcga aacctgcgct ttcgatttgc
     3481 tgaacgccca atacatccgc gaaatcgagc ccggcgaaat gctccgcatt tcccgctccg
     3541 ggctcgagtc catacacttc gctccggaaa aaccccacca gttctgcatt ttcgaacacg
     3601 tctatttttc gcggccggac agcaccatct tcggccgcag cgtaaatgac agccgtgaaa
     3661 tgctcggccg cctgttggcg cgcgagcatc ccgtcgatgc cgacatcgtc gttccggttc
     3721 ccgattccgg cgtgcctgct gcggtcggtt acgccatgga gtcccgcatt ccatttcgca
     3781 tgggcttgat tcgcaatcat tatattgggc gcacttttat cgaaccgtcg caggccattc
     3841 gcaatttcgg tgtgaaactg aaactcaatc ccattcgcgg cctcatggaa ggcaagcgcg
     3901 taatcctggt tgacgattcc atcgtccgcg gcaccaccag ccgcaaaatt gtccgcatgg
     3961 ttcgcgaagc cggcgccaaa gaagtccacg tgcgtatctc ctgcccgcca accatttctc
     4021 cctgctatta cggagtggac acaccaaccc gcgaggaact gatcgcgtcc tgcaatactc
     4081 ccgaagagat ttgccgtttc ctcggtgctg actccctcgg ctacatttcg ctgaccggtt
     4141 tgcgccaggc cgtcaacgac accaaaggca atttctgtac gtcttgttat acggggaact
     4201 accccacgga tttagtgcag ttggaagtgc gcgaaggagc gaatctcggc aagctgaccg
     4261 acagcgctcc ggtaatggtg gaacgtgccc cgtaatggtg aaatgtgccc gtgatggtga
     4321 aatgtgaagt ataagaaaga acaaagcaag aaaccgcccg acaagagaaa acagcccgaa
     4381 ctcaccatgg aacaaaaagt ctggcgcgaa gtcgtcgcgt ggttctgggt cggcttggtg
     4441 ttcctgctca tcaacggggc cgtgggccag gcccgcgtga ttccttccga gtccatgcag
     4501 aacaccttgc tggtcggcga ccacctcatc atgagtcgca tgggctacga cgccggcatt
     4561 ccgttcacga acgtgcatgt gccgctgtgg cgaaatccca agcgcggcca aatcgtaatt
     4621 tttcggccgg tgattcccgg cgaaacgtct gacgtcatca agcgcgtgat cggattgccc
     4681 ggcgaaaccg tagacgtgcg cgacggcagc gtgtggatta acggaacgcg tttgcaggag
     4741 ccgtacaccg tcggccgtac cgagccgggt gatcccggaa tgttccgcgg ggctctggcc
     4801 ggccaaaaca atgttctgcc gtacaaaatt ccgcccgatt gctatttcgt aatgggtgac
     4861 aaccgcggca attctctgga cagccggttt gttggctgca tcccccgcgc caacatcgtt
     4921 ggcaccccgg tgatgattta catgtccatc gatacgccct ccgagtcgtg gggttcgggc
     4981 gcgatcaccg accgcgtttc cgcatacgcg aatgcactga tccatccgag tgaggttcgc
     5041 tggcgcaggt tgtttcggtt attctagttg tagagcagcc ggctggatcc ggcgccggtg
     5101 ggaccgtgaa ggccgaaaaa gtccaaagca aaccccaaga caaactgacc atggagcaga
     5161 aggtccgccg cgaaattatc gcgtggttct gggtcggctt ggtttttctg ctgatcaacg
     5221 gcgccatcgg acaagcgcgc gtgattccct ccggttccat ggaaaatacg ctgctgatcg
     5281 gcgaccacct catcatgagc cgcgttggct acgacgcagg aattccgttc acaggatggc
     5341 acgtgccgct gtggcgcaac ccgaagcgtc aacagatcat tattttcaag ccgccatttg
     5401 acctgtcgca gcccgattat gtgaaacgcg cgatcggctt gccgggcgac atcatcgata
     5461 ttcatgacgg cgcggtgtgg atcaacggcg agcggctggc ggaaacctac acgctcggaa
     5521 aaaccgaacg cgtggatccc ggtctttatg ccggcgcgtt cgcgggaatg ccgcaggtga
     5581 tgccctacaa aattcccgat aattgttatt ttgcgatggg cgacaaccgg cagaattcgt
     5641 tcgattcgcg tttctggggc tgcgtccccc gcaaagacat catcggcacg ccggtgctga
     5701 tttacatgtc cctgaacgcg gggacggagc cgtggcaacc gggcaaaatc ggcgaaagat
     5761 tcctggccta cctgaatgga attatccatc cggggacggt gcgctggagg cggttgttcc
     5821 acgttttcta gcgggtattc tgagcgggca aacgaggtta caggctgcgg cgaatccccc
     5881 cgtgccgcga gatttgacgc gagcttaaaa caaaggttgc cggaaccatc catgagatac
     5941 gccgacgcag gagtgaatat cgctgtcgcg gatgaagcca aaagccgcat acgccacctc
     6001 gccactcgca cgtttaccaa tggcgtgctg ggaggaatcg gcagcttcgg cgcattgttc
     6061 gcgctggaca tgaaaaagtt caaggaaccg attctggtgt cgagcgcgga cggcgtcggc
     6121 accaagctga aagtcgccat cgcgatgaac gtgcattcca ccgtcggcgg cgacctggtg
     6181 aatcactgcg tgaacgacat tctggtgcaa ggagccgagc cgctgttctt tctggactat
     6241 ctggcgctcg gcaaaatgga tccacgcgtc gttgagcaac tcgtagatgg catgagccgc
     6301 gcttgccgca aagcaggctg cgctctaatc ggcggcgaaa cggcggagat gccgggcttc
     6361 tatgccgccg gagattatga cctggccgga tttattgtcg gcgcggtcga aaagaaaaat
     6421 ctgctgacgg gaaaaaacgt gaagccgggc gacacgctgc tggcgctgcc ctcggcgggg
     6481 ctgcatacca acggttattc gctggcgcgc aagctggcgt ttgaagtggc caagctaaag
     6541 ccggaaacgt atgtagcgga agtggggaac aagatcggcg cggaattgct gaagccgcat
     6601 ctttgttatg caccagcgct gcgcacgatt ttggcgaacg gctgggtttc ggctctggcg
     6661 cacattaccg gcggcggaat tcccggaaac ttgccgcgtg tgttgccggc cggagtgaag
     6721 gcgcagatcg atctggcgtc gtggcctgtg ctgcccatct tcaagtacct ggcgaaggtc
     6781 gggaagatgg attcggatgc gctgctggag tcgttcaacc tgggcgtggg catggtgctg
     6841 gtggtgccgt cggcatacgt aaaaaaggtg gaagcggacc tgaagcgccg ccgcgagaag
     6901 ttcttccaga tcgggcgcat cgagcgcggg gataccgcaa aggcacgggt cgtatattcg
     6961 ggcgcgctga gtctgtagca gctctgattt ttggcgtcct cctacgtcgg agctgaagct
     7021 ccaacctcct aaagcggatt gaaagagcag agtctggcct gaatggaggc aggatctagt
     7081 cgccatagtc gcagcgtttt aacgccaaac cgattcgttt gtgccggaga gggcacggtc
     7141 gaggtaggtt gcggcgctaa tcaaagcgag gtgggtaaaa gcctggggaa agttgccaag
     7201 atggcggcca ttaacgccaa gttcttctga gtagagacca agatggttgg cgtagcccag
     7261 aagcttttcg aacatcaact gtgccttctc caattcgccg gcgcgagcaa ggcattcgac
     7321 ataccagaag gagcacgcgg tgaagcttcc ttcgctaccg ggaaggccgt cgacatgact
     7381 ttgttccact tgataacgac gcacgagcgt gtcttcgact agatgtttct caatcgctcg
     7441 caaggttgaa agccacatcg ggtcgaccgg actgatgaac ctcatcatgg gcatcagcag
     7501 cgccgaagcg tcgaggtctt tggttccctt tacctgaacg aaggattgca ggtcggcgtt
     7561 ccagaaattc gtaaagatat cactgcgaat ttcgtctcga atgcgttgcc aaacatcaag
     7621 aggtccggag agcgagcgtt tgtgagcgag gcgcaacccg cggtcgaaag cgacccagca
     7681 catgacgcga gagtgcagga attctcttgc gccgccgcga acttcccata ttccctcatc
     7741 gggacgccgc caattgttgc caagccattc gaggatccgc tgcacctctt gccagccctg
     7801 atgagagatg ggccggccat atttgttcga caaatagatg gcatccatca tttcgccgta
     7861 gatgtcgagt tgcagctgtt tgaaagctgc gtttccaatg cgcaccggcc gcgaattttc
     7921 gtagccgaag agatgatcga gcgaaatttc ctcgaggtcc tgcctgccat cgattccgta
     7981 cattacctgc agcggaccgc gctgggcgtc gtcacccaag cggcccttga gccagtcaat
     8041 gaatccttcc gtttcatcga cgaatcccaa gcggatcagc gcgta
//
LOCUS       DASTPM010000774         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_950742, whole genome shotgun sequence.
ACCESSION   DASTPM010000774 DASTPM010000000
VERSION     DASTPM010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950742"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1872)
                     /locus_tag="VFR42_13670"
     CDS             complement(184..1872)
                     /locus_tag="VFR42_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEU0370261.1"
                     /translation="MSLCILLPTAVLAASDDASPDPTKEPGLGPILGYITKSWDTLTR
                     SMEECTTVVDPKLAENSVLYLPAEMTVPAQVAEMQKRCHIQVKSLPEKITRPGQIDTA
                     KLDPQGLLYLEHKYVVPGGRFNEMYGWDSYFIVRGLVRDGRIELAKGMIENFLFEIEH
                     YGTILNANRSYYLSRSQPPFLTSMILSVYEAEKAAGHEDRKWLERVYGYAGKDYEMWI
                     SAPHTADGTGLSRYYDFGNVPAPESLKDETDHYRRVAAYFVMHPELDRGYLEREPIGK
                     PKRDAVGKHYAVDVCESPLMAAAKPDCEDAAGVTLSQEFYRGDRSMRESGFDVSFRFG
                     PHGAGTHHYAAVCLNSLLYKYEKDMETMSTMLGRAKDAASWKQRAAERGENIQKYLWD
                     VEQGMFFDYDLHHKERSTYIYITTFYPLWAGAATKEQAAAIMKHLGKLEQPGGLVMSP
                     YESEGQWDFPYAWAPTQLLAIEGMRKYGFNTDADRISYNFLSMIAANFRHDGTIREKY
                     NAVTRSSETAVKAGYNINVVGFGWTNAVFLVFLHDLPAAMVEKLAAEQLQPQSN"
     gene            complement(2080..3474)
                     /locus_tag="VFR42_13675"
     CDS             complement(2080..3474)
                     /locus_tag="VFR42_13675"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU0370262.1"
                     /translation="MSTASTDLIIEIAYMIATALFILSLKWLSSPTTARRGVFAGEAG
                     MLLAIGGTLLHRGIVDYKWIAIALVLGTVIGIPLGRVHMTAVPQRTALSHAFGALCVT
                     LVGTAEFYLRAPDVPKFLMAVLSMEVILGSLTLTGSLMAAGKLQELLPQRPIAYKGQN
                     FVNLSLLGIAIIIAVMLVLDPSKMYLYPLIVVIPLIFGVMMIIPIGGADMPTVISLLN
                     SYAGLSAAAMGFVLDSKLLIVAGALDGASGFILSVNMSKAMNRSFTNVLFGAFGQEQT
                     AVAGKEEAKPVRSATAEESAAILAAANKVVIVPGYGMAVSQAQHKVRELYDALSKRGV
                     DVKFGIHPVAGRMPGHMNVLLAEADIPYDKLLDMEEINGDFQQTDVALVIGANDVTNP
                     AARSDTSSPIYGMPILDVDKARTVMVIKRGMSAGFAGIENPLYYLDRTLMLFGDAKGF
                     VGNIVRELTGGGHT"
     gene            complement(3471..3767)
                     /locus_tag="VFR42_13680"
     CDS             complement(3471..3767)
                     /locus_tag="VFR42_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0370263.1"
                     /translation="MKLDLISSLYVFMLAAFIGFEVIRRVSPLLHTPLMSLTNALDAI
                     AVVGAIILAGEHKTTLATILGTVAIVSATSNIVGGFIITDRMLKMFKSSGAKKA"
     gene            complement(3772..>4825)
                     /locus_tag="VFR42_13685"
     CDS             complement(3772..>4825)
                     /locus_tag="VFR42_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU0370264.1"
                     /translation="SAGYPDSEYADRGAKVVASRPDMFAAADIIVQVLCYGSNDKTGR
                     ADLPLLRSGQALVGFLRPLGTVETLREIARTGATAFSVELMPRTTRAQSMDALSSMAT
                     ICGYKAVVMAADTLPRIFPMLTTAAGTITPGRVLIIGAGVAGLQAIATARRLGAVASA
                     YDMRPAAKEQVQSLGGRFVELPIEAEDAQDARGYATAQGEEFYRKQRELLGEAVAAND
                     VVITAAVIPGKTSPILVTKEMVARMAPGSVIVDLAAERGGNCELTRGGEIVVEHGVTI
                     IGAFNLASSVPYHASQMYAKNVTAFLLNLVKDGKLQINEADEIHRETLLTYGGEIVNA
                     RVREFFGLPAQARKGA"
BASE COUNT          993 a         1615 c         1173 g         1044 t
ORIGIN      
        1 gccgctgcca accgtatggg agcctgggtc gcgtcgcagg aaggcgccat gcctcccgcc
       61 gaccctgaaa ttctcaagga agtccgcgcc gtggcggcat gaagcgcgac agcacagtct
      121 tcagtctgtg cggcgattgc aagcgggagg tattctcatt cgcgtcgtcg cgagcaagcc
      181 aagtcagttg ctttgtggtt gtaattgttc ggcggccagc ttctccacca ttgccgccgg
      241 caaatcgtgc agaaatacca ggaacaccgc gttggtccac ccgaatccca ccacgttgat
      301 gttgtagccg gctttcaccg cggtttccga tgaccgcgtc actgcgttgt atttctcgcg
      361 aatggttcca tcgtgccgga aatttgcagc gatcatgctt aaaaaattgt agctgatccg
      421 gtcggcatcc gtattgaatc cgtacttgcg catcccctca atcgccagca gttgcgtcgg
      481 cgcccacgca taagggaaat cccactggcc ctccgactcg taaggactca tcaccagtcc
      541 gcccggctgc tccagcttgc ccagatgctt catgatcgca gccgcttgct ctttcgtcgc
      601 cgcacccgcc cacagcggat aaaacgttgt gatgtaaatg taagttgacc gttccttatg
      661 gtgcaggtcg taatcaaaga acatcccctg ttccacgtcc cacaagtatt tctgaatgtt
      721 ttctccgcgc tccgcggccc gctgtttcca acttgccgca tccttcgccc gccccagcat
      781 cgtgctcatc gtttccatgt ctttttcgta tttgtagagc aggctgttca agcacaccgc
      841 cgcgtaatga tgcgtccccg cgccgtgcgg cccgaaccgg aatgatacgt cgaatcccga
      901 ttcgcgcatc gagcggtccc cgcgataaaa ctcttggctc agcgtcacac cggccgcatc
      961 ttcgcaatcc ggtttcgcag cggccatcag gggagattca caaacatcga ctgcataatg
     1021 ctttccgacc gcatcgcgct ttggctttcc gatcggctca cgctccaggt atccgcggtc
     1081 cagctccggg tgcattacga aatacgctgc cacccgccgg tagtgatccg tttcatcttt
     1141 taaactctcg ggcgccggca cattcccaaa atcgtagtag cgcgagagcc cggtcccatc
     1201 cgctgtgtgc ggcgcgctga tccacatctc atagtctttg ccagcgtaac catacacgcg
     1261 ctccagccat ttcctgtctt cgtgtcccgc agctttttcc gcttcataca cactcaaaat
     1321 catcgaagtc aagaatggcg gctgcgaccg gctcaggtaa tagctgcggt tcgcgttcaa
     1381 gatcgttccg tagtgctcga tctcaaacaa aaagttttcg atcatccctt tcgctaactc
     1441 aattcgtccg tcgcgcacca gtccgcgcac gataaaatag ctgtcccatc cgtacatttc
     1501 attgaaccgc ccgccgggca ccacatactt gtgctccagg tagagcagtc cctgcgggtc
     1561 cagttttgcc gtatcaattt gtcccggccg cgtgattttc tcgggcagcg acttcacctg
     1621 gatgtggcag cgcttctgca tctcggcgac ttgcgccggt accgtcatct ccgccggcag
     1681 atacagcacg gaattctccg ccagtttcgg atccaccacc gttgtgcact cttccatcga
     1741 gcgcgtcagc gtatcccacg atttggtgat gtagccgaga attggcccca gccctggttc
     1801 cttcgtcgga tccggcgaag catcgtcaga cgccgcgagt accgcggtcg gcagcaagat
     1861 gcacagtgac aaccagaaga agaccatcca gcacctggaa aaatgccgta aatttgtcat
     1921 ggagatgact cgaggcaggg cgcgcattac aaccaccaga atagatgcaa actattgaaa
     1981 agtaaaggac ttagttcgtt atgaaagtcg catgaacgac caaaatcccg cgcggtttct
     2041 gcccaaatgc ctgtccattc cgtcgccaaa accgaacggc taagtatgtc ctccgccggt
     2101 caattcacgc acaatgttcc ccacaaatcc cttcgcatct ccgaaaagca tcaacgtcct
     2161 gtccagatag tacagcggat tttcaatccc cgcgaaaccc gccgacatac cgcgcttgat
     2221 caccatcacc gttcgcgcct tgtcgacatc caggatgggc attccataaa ttgggctgct
     2281 cgtatcgctg cgcgccgcgg gattcgtcac gtcgttcgcg ccgataacca gtgcgacatc
     2341 cgtctgctga aaatctccgt taatctcttc catatccagc aatttgtcgt agggaatgtc
     2401 cgcttccgcc agcagcacgt tcatgtggcc cggcatccgt ccggccaccg gatgaatgcc
     2461 aaacttcacg tccacgccgc gcttgcttaa cgcgtcgtac aattcccgta ccttgtgttg
     2521 cgcctgcgac accgccatgc cgtagcccgg cacaatcacc accttgttcg ccgccgccag
     2581 aatcgcagca gattcttccg ccgttgcgct gcgtaccggc ttggcttctt cctttcccgc
     2641 aacggctgtc tgctcctgcc caaatgctcc aaacagcacg ttggtgaacg accggttcat
     2701 ggctttcgac atgttcaccg aaagaatgaa gcccgaagct ccgtccagcg cgcccgccac
     2761 gatcagcagc ttgctgtcca gcacaaatcc catcgccgcc gccgacaaac cggcatacga
     2821 attcagcagc gaaatcaccg ttggcatgtc cgccccgcca atcggaatga tcatcatcac
     2881 gccgaaaatc agcgggatca ccacgatcag cgggtacaaa tacattttcg aaggatccaa
     2941 taccagcatc accgcgatga tgatggcgat gcccagtaat gacaggttga caaaattctg
     3001 tcctttatag gcaatcggcc gctgcggcag caattcttgc agcttgcctg ccgccatcaa
     3061 actccccgtc agtgttaacg aaccgagaat cacttccata gacagcacgg ccatcaaaaa
     3121 cttcggcaca tcgggcgcgc gcaaataaaa ttccgccgtg cccaccagcg tcacgcacag
     3181 cgcgccaaac gcgtggctca acgctgtccg ctgcggcacc gccgtcatgt gcacgcggcc
     3241 cagtggtatg ccgatcacgg ttcccagcac cagcgcgatc gcgatccatt tgtagtcgac
     3301 aatcccgcgg tgcagcagcg tgcccccaat cgccagcagc atgcccgctt ccccggcaaa
     3361 taccccgcgc cgcgccgtcg tcggcgaact cagccacttc agcgacagga tgaacagcgc
     3421 tgtcgcaatc atgtacgcga tttcgatgat caagtcggtc gaagccgtgc tcatgccttt
     3481 ttggccccgc tggacttgaa cattttcaac atgcggtcgg tgatgatgaa tccgcccacg
     3541 atgttgctgg tcgccgacac aatggcgacc gttcccaaaa ttgtcgccag cgtcgttttg
     3601 tgttcgccgg ccaaaatgat tgcgcccacc actgcaattg cgtccagcgc attggtcagc
     3661 gacatcagcg gcgtgtgcag cagcggcgaa acccgccgga tcacttcgaa tccgatgaac
     3721 gccgccagca tgaacacata caagctggat atcagatcga gcttcacgac atcacgctcc
     3781 cttcctcgcc tgcgccggca gtccgaaaaa ttctcgcacc cgcgcattca caatttctcc
     3841 gccatatgtc aacagcgtct cacggtggat ttcatcggct tcgttaatct gcaacttgcc
     3901 gtccttcacc aggttcagca aaaatgccgt cacgtttttc gcgtacatct ggctggcgtg
     3961 atagggaacc gagctagcca gattgaacgc cccgatgatc gttacgccgt gctccaccac
     4021 gatttcgccg ccacgcgtca gctcgcaatt tccgccgcgc tccgccgcta gatccacaat
     4081 caccgaaccc ggcgccatac gcgctaccat ttctttagtc accaggatcg gcgaagtctt
     4141 tccgggaatc accgccgcag tgatcaccac gtcgttcgcc gccaccgctt cacccagcag
     4201 ttcgcgctgt tttcgataaa attcttcgcc ctgagcggtc gcatatccac gcgcgtcctg
     4261 agcgtcctcc gcctcaatcg gcaattcgac aaaccgtccg cccagacttt gcacttgctc
     4321 cttggccgcc ggccgcatgt cgtatgcgga agccaccgca cccagtcgcc gtgctgtcgc
     4381 aattgcctgc aatcccgcca cgcccgcgcc gatgattagt acacgtcccg gcgtgatcgt
     4441 gcccgctgct gtcgtcagca tcggaaatat tctcggcaac gtgtcggcag ccatcactac
     4501 cgctttatac ccgcagattg tcgccatcga cgacagtgcg tccatgctct gcgctcgcgt
     4561 cgtgcgcggc atcagctcta cggagaacgc tgtcgctccg gttcgtgcga tctcccgcag
     4621 tgtctccact gttcccagcg gccgcaaaaa tcccaccagc gcctggccgc tgcgcagcag
     4681 cggcaaatcc gcccggcccg ttttgtcatt tgatccgtag caaagaacct gcacgataat
     4741 gtccgccgcc gcaaacatgt cgggccggct cgccaccacc ttcgcgccgc ggtccgcata
     4801 ctcgctgtcc ggatagccgg ccgat
//
LOCUS       DASTPM010000775         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_345423, whole genome shotgun sequence.
ACCESSION   DASTPM010000775 DASTPM010000000
VERSION     DASTPM010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345423"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFR42_13690"
     CDS             complement(<1..224)
                     /locus_tag="VFR42_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0370265.1"
                     /translation="MREVFGPGGMLEKSMFGGYEHRAAQLEMAEAVHDAFQGKQHAIV
                     EAGTGTGKTLAYLLPAICSGRRVVISTATKS"
     gene            752..1465
                     /locus_tag="VFR42_13695"
     CDS             752..1465
                     /locus_tag="VFR42_13695"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0370266.1"
                     /translation="MPHVQKHAPGDFCWVELATTDQPAAEAFYAKIFGWSANNMPMGP
                     DGVYTIFQLEGRDAAAACTLRPDELQRGVPPHWNLYIAVESADASAARAADLGGTVLA
                     AAFDVGDAGRMAILQDPTGAVFAVWQAKRNPGTGITGTHGTLCWADLSTPDQARVGDF
                     YSGLFGWHIMKEDEDPAHNYWHIKNGEEFIGGIPPASNHQPGAPAHWLAYFAVSDCDA
                     TASGAKELGAKLYMPADGF"
     gene            1939..>2562
                     /locus_tag="VFR42_13700"
     CDS             1939..>2562
                     /locus_tag="VFR42_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370267.1"
                     /translation="MRLASKLFLFAILGFALASCSGVPGGGCVANCSTGGSVSVVLTA
                     TPPPPTSALSIQAFTATITGLTLTPSGGGSPVTIHLNSTAYIAEFNRVTSDSTLLASS
                     VSIPSGSYNQVTITFAAPRVTFCTQANPGVPGCANATLASIVGLPGNVTASTNLTVAN
                     NALTGILLNVNLGNALTLTGQTIAGVDLTVANTFSASLLPPAASATDL"
BASE COUNT          467 a          909 c          616 g          570 t
ORIGIN      
        1 gatttcgtcg ccgtagaaat caccacccgc cgcccactgc aaatcgccgg caacaaatac
       61 gccagcgtct tcccggttcc cgtccccgcc tccacaatcg cgtgctgctt cccctgaaac
      121 gcgtcgtgca ccgcctccgc catctccagt tgcgctgccc gatgctcgta tccgccaaac
      181 atgctcttct cgagcattcc ccctggcccg aacacttccc gcatctcaac ttgtccgccc
      241 gcaactggcg ttatttcgtc gctttgactc gacgactttg cgtttctctt ggttgcccca
      301 gccttgcttc gtaagggtag gtcttcttcc tccaacgaga cataccgtct ctctaaccca
      361 agctccccag tgtccgggat aaattcagac gcagacctac ccgcggcctc aacggccttc
      421 cttcgccgtt tccctttcgt cgcgactttc tgtgtttgct cttccagaac ttctctctcc
      481 tgacccatcc aaccaatcct cgacaataac acaatggcga attctccccc ggaacacctg
      541 cccgccgcgg cgggttcccg ttgtgctcgt gtttgagtga aaccggggtc ggacctttag
      601 gtccgacatg cataactcag gagaaaactc gctaagcgcg gatacttttt ctagaatgct
      661 tgcctgttta gctcgcttct acttttgact ttcgctgtca acttggtttc cgccccttgc
      721 ctccaccccc gctcgggagt accttcttcc catgccacac gtacaaaaac acgcacccgg
      781 cgatttctgc tgggtcgagc tcgccactac cgatcagcca gccgctgaag ccttctacgc
      841 caaaatcttt ggctggtccg ccaacaacat gcctatgggc cctgacggcg tttacaccat
      901 cttccaactc gagggacgcg atgctgcagc agcctgcact cttcgtcccg atgagcttca
      961 gcgcggcgtg cctccgcact ggaatctcta catcgccgta gaaagcgccg acgccagcgc
     1021 cgctcgcgct gccgatctgg gtggcacggt cttggcggcc gccttcgacg ttggcgatgc
     1081 cggtcgcatg gccatccttc aggatcccac gggcgcggtt tttgctgtct ggcaagccaa
     1141 gaggaatccc ggcaccggaa tcactggcac gcacggcacg ctctgctggg ccgacctcag
     1201 cacgcccgat caagcccgcg tgggagactt ctattctggc cttttcggct ggcacataat
     1261 gaaggaggac gaagaccccg cccacaacta ctggcacatc aaaaacggcg aagagttcat
     1321 tggcggcatc ccgcccgctt ccaaccatca gccgggcgct ccggcccatt ggctcgccta
     1381 cttcgccgtt tcggattgtg acgctaccgc ctccggggcc aaggaactcg gcgccaagct
     1441 ctatatgccc gccgacggat tttgaggatg tgggccgcat ttccatcatg gctgacccgc
     1501 aaggcgccac cttcgccact ttcaaggccg tcgcccaccg cgcggcggcg ccctaatcct
     1561 tgcgtcgttg caattttctt cgggcggccc gagctttgct gttcatctgc cagttcgggc
     1621 tcgcctaagc actgcagaat caatacttac agaatgcccc cgcggtatcc cctttagaat
     1681 cattatttac gaaagaaaac cgggccaggt atcgccttcg cggaccacgc agctcccact
     1741 ctgaatttgc ggtcaccttg cgcgattggg caagtccacg ctatttactg cgcgtcctgc
     1801 gacggccccg cggcgtacat tggaaagaaa agcatgctcg ttccagggct tgctcgacgg
     1861 cactggaagg aataggaaac ggacaggcca gccacacggt ggaactggcg aagcagactt
     1921 cacacacggg gtagtccaat gcgcctggcc tcaaaactct tcctgtttgc gatcttggga
     1981 tttgcgctcg cgtcctgcag cggtgttccc ggcggcggct gcgtcgccaa ttgttccact
     2041 ggcggctccg tctctgtcgt cctcaccgcc acgccgcctc cgccaacttc cgctctctcc
     2101 attcaggctt tcactgccac catcacaggg ctcacactca ctccctccgg cggcgggtcc
     2161 ccggtcacca tccatctcaa ttccactgcg tacatcgccg agttcaatcg cgtcaccagt
     2221 gactccaccc tgctcgcctc tagcgtctcc ataccttccg ggagctacaa tcaggtcacc
     2281 atcacctttg ctgcgcccag agttacattc tgcacgcaag ccaatccggg tgtgccaggc
     2341 tgcgccaacg ctacactggc ttcgatcgtg ggattgccag gcaacgtgac cgcctccacc
     2401 aatctcaccg tcgccaacaa cgcgctgacc ggcattttgc tcaacgttaa tcttggcaac
     2461 gcgcttacgc taactggtca gaccatcgcc ggcgtagacc tcacggtcgc caatactttt
     2521 tcagcgtcac ttcttccgcc cgccgcttcg gcaaccgatc tt
//
LOCUS       DASTPM010000776         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1124127, whole genome shotgun sequence.
ACCESSION   DASTPM010000776 DASTPM010000000
VERSION     DASTPM010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124127"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1320
                     /locus_tag="VFR42_13705"
     CDS             220..1320
                     /locus_tag="VFR42_13705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0370268.1"
                     /translation="MKREGPGSRVRTIQVAVLSLCICGFLLFASPSFAQSSGDPEENK
                     AEVKPPLTPAPVNPDKAERTPLKVSAKDDVRHDTPGLTIKPGKVLHADVDVALVNVTV
                     TDPYNRLVTGLEPDNFRVFEDNSEQEVVSFSSEDVPISIGVIFDLSGSMSNKIAKARE
                     AAIQFFKTANPADEFFLVSFNERAELTSAFTNSVEDLQSRMLTTSPKGRTALLDAIYL
                     GLSQMRGARNAKRALLILSDGGDNHSRYNESDIKRLVKEADTQLYAIGIFDPLGNRNR
                     TPEELNGPSLLTDITDMTGGRVFSVERLEDLPDIATKVGMELRNQYVLGYHPSNKAHD
                     ARWRKIKVKLRAPKGLPPLNVFAKTGYYAPSH"
     gene            1346..2350
                     /locus_tag="VFR42_13710"
     CDS             1346..2350
                     /locus_tag="VFR42_13710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0370269.1"
                     /translation="MLPALSLAFLLFASSAVFAQDNPLNLPQAQPAPPGQANQQGGSI
                     KVDVSLVVLHTTVLDDRGKFVDGLKEENFRVFEDKAEQKLSVFKREDVPVSMGLVIDN
                     SGSMRDKRPRVNEAAVTLVQSSNPQDEAFVVNFNDDYYLDLDKDFTSSIPELKEALER
                     IDARGSTALYDAIIGSMDHLKKSRKDKKVLLVVTDGEDNVSRNTLEKTLREIQKANVV
                     IYTIGIFSDDDKKGRKKASRALKDIAAASGGMSYFPENVSDVHSICEQVARDIRNQYT
                     LGYYPTNTKKDGTFRTVNVDVIPPRGKGKLSARTRNGYFAPGTAAASNEAPSAATAAR
                     "
     gene            complement(2385..>3012)
                     /locus_tag="VFR42_13715"
     CDS             complement(2385..>3012)
                     /locus_tag="VFR42_13715"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU0370270.1"
                     /translation="TAAGAEARVAGMRRDGDDDKELFIEVVEYSDGVTICAVYAKDKT
                     PGRCHRGGVRSESSSWHGHHAKLNFDVQVPRGVRFNALTTNGGVHCRDLNSVVEAATT
                     NGNVEVSTSEWASAKTTNGGVRVSMGNAKWTGELELATTNGRVNVTLPASAEFEVNAA
                     TTNGGIHTDFPITVQGSFSSKELSGKVGAAGRYLKVATTNGGIDLKKS"
BASE COUNT          649 a          857 c          894 g          612 t
ORIGIN      
        1 agctcctgag cactaaagtt caggcgggct gcggcctaag tgtcgcaagg agatgctacc
       61 acgcgggacg gcggcggctg gcgcgccggc agataccttg gattcctgat tccggccaag
      121 ttacaacgat ttgacttgca ctggtatttt cggctgagta aaatccgttt agtccgaatc
      181 cgcgcggccg ttccagcaag ccgggacgat gggggagccg tgaagcgcga aggtccaggg
      241 tctcgcgttc gaaccatcca ggttgccgtt ctttccttgt gcatatgcgg attcttgctg
      301 ttcgcctcgc cttctttcgc ccagtcttcc ggcgatcccg aagaaaataa agccgaagta
      361 aagccaccac tgacgcctgc gcccgtgaac cccgacaagg cagagcggac gccgctgaaa
      421 gtttcggcga aagacgacgt acggcatgac acacccggac tgaccatcaa accggggaaa
      481 gtgctgcacg ccgacgtgga cgttgcgctg gtgaatgtta cggtgaccga tccttacaac
      541 cggctggtca ccgggctgga gccggataac ttccgcgttt ttgaggacaa ctcggaacag
      601 gaagtggtgt cgttttcctc cgaggatgtg ccgatttcga tcggcgtcat tttcgatctc
      661 agtggaagca tgtccaacaa gattgcgaag gcgcgcgaag cggccattca attcttcaaa
      721 accgccaatc cggcagacga atttttcctg gtgagtttca acgagcgcgc agagctgacc
      781 agcgcgttta ccaacagcgt ggaagatttg caaagtcgca tgctcacgac cagccctaaa
      841 gggaggaccg cgctgctcga tgccatctat ctcggattga gccagatgag gggtgcacgc
      901 aacgccaaac gtgccttgct catcctatcc gacggagggg ataatcatag taggtacaac
      961 gaaagcgaca tcaagcggct cgtaaaagaa gcagatactc agctctatgc catcggaatt
     1021 ttcgacccgc ttggaaatcg caatcggacc ccggaagaac tgaacgggcc gtcgctgctg
     1081 actgacatta ccgatatgac cggcggacgc gttttctcgg tcgagcggct cgaagatctc
     1141 ccggacatcg ccaccaaggt tggcatggag ctgcgtaacc aatatgttct gggataccat
     1201 ccgagtaaca aggcgcacga cgcccgctgg cgcaagatca aagtcaagct gcgcgctcct
     1261 aagggattgc cgcctctcaa cgtctttgcc aaaactggct actacgctcc cagccactaa
     1321 gcggccctgc aacggcgtgc agctcttgct gcctgccctc tcgctagcgt tcttgctctt
     1381 cgcatcctcc gctgttttcg cgcaggacaa tccgctcaat ttgccgcagg cacagcccgc
     1441 gcctccgggg caggcgaacc agcagggggg cagcatcaag gttgacgtga gcctggtggt
     1501 gctgcacacg acggtgctgg atgaccgtgg aaagttcgtg gatggcttga aggaagagaa
     1561 tttccgcgtg ttcgaggaca aagcggaaca gaagctttcc gtcttcaagc gcgaagatgt
     1621 gccggtcagc atgggcctgg tgatcgacaa cagcgggtct atgcgggaca aaaggccgcg
     1681 ggtgaacgaa gctgcggtga ctttggtaca aagctcgaat ccgcaggatg aagctttcgt
     1741 tgtgaatttt aacgacgact actatctgga tctcgataag gacttcacca gcagcatccc
     1801 cgaattgaag gaagcgctcg aacgcatcga tgcgcggggc agcacagcgc tctatgacgc
     1861 catcatcggt tctatggacc acctgaagaa gtcgcgcaag gacaagaagg tgttgctggt
     1921 ggttacggac ggcgaggaca acgtcagccg caatacgctg gaaaagacgc tgcgcgagat
     1981 tcagaaagcc aatgtggtca tctacaccat cggaattttc agcgacgacg ataagaaggg
     2041 gcgcaaaaaa gcttcccgcg ctcttaagga cattgccgct gcatccggcg gcatgtcgta
     2101 tttcccggaa aacgtcagcg acgtgcacag catctgcgaa caggtggcgc gggatatccg
     2161 caatcagtac acgctcggct actacccaac caacaccaag aaggatggaa cgttccgcac
     2221 ggttaacgtg gatgtgattc cgccgcgtgg aaaaggcaaa ctatccgcgc gtacgcgtaa
     2281 tgggtacttc gctccgggca ctgcagctgc gtcgaacgag gcgccttctg cagcgactgc
     2341 ggcgcgatag ggcgacccct cggacgcaat tctgcttgca acttctagct ctttttcaag
     2401 tcgatgccgc cgtttgtggt ggccactttc aggtagcggc cggccgcgcc gacctttccg
     2461 ctgagttcct tcgagctgaa gctgccttgg acggtaatcg ggaaatcggt gtggatccca
     2521 ccgttggtcg tcgccgcatt cacttcaaat tcggctgagg cgggcagcgt tacgttgacg
     2581 cggccattgg tggtagcgag ttccagttcg cccgtccact tggcgttgcc catggaaacg
     2641 cgcaccccgc cgttggtggt tttcgccgaa gcccactcgc tggtggaaac ttccacgttt
     2701 ccgttggtgg tggcggcttc gacaacgctg ttgaggtcgc ggcagtgcac gccgccgttg
     2761 gtggtcaagg cgttgaagcg gacgccgcgt ggaacttgca catcgaagtt aagcttggcg
     2821 tgatgaccgt gccagctgct ggattcggag cgcacgccgc cgcgatggca gcggccgggc
     2881 gttttgtctt tggcatagac cgcgcagatg gtgacaccgt cggagtattc cactacttcg
     2941 atgaacagtt ccttatcgtc gtcgccgtcc cggcgcatgc ccgcaacgcg agcctcggct
     3001 ccggccgcag ta
//
LOCUS       DASTPM010000777         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_720360, whole genome shotgun sequence.
ACCESSION   DASTPM010000777 DASTPM010000000
VERSION     DASTPM010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720360"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1285)
                     /locus_tag="VFR42_13720"
     CDS             complement(200..1285)
                     /locus_tag="VFR42_13720"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU0370271.1"
                     /translation="MSEAARTSPTTSPAKAFAAPSPTGALAPHTIARRAPRPQDVQLE
                     ILFCGVCHSDLHQVRNEWKEVMPTVYPCVPGHEIVGRVTKVGSAVKKFKEGDIAAVGC
                     LVDSCRICSSCKAGEEQYCENTFTLTYNSEDKILGGVTYGGYSDSIVVDESFVLRVPN
                     GLDPAGTAPLLCAGITTFSPLHHWKVGKGQKVGIVGLGGLGHMGVKFAHALGAHTVLF
                     TTSAGKTADAKRLGADEVVISKDADAMQKHAGSFNFILDCVSAQHDINAYLNLLRRDG
                     TVCLVGAPEKPLPANAFNLLFGRRSLSGSAIGGLRETQEMLDFCGEHGITADIEKIRI
                     QQINEAYDRLLKQDVKYRFVIDNSTLQ"
     gene            complement(1448..>2657)
                     /locus_tag="VFR42_13725"
     CDS             complement(1448..>2657)
                     /locus_tag="VFR42_13725"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0370272.1"
                     /translation="VSGLAGASPFDPPRSKPYPMPPLPVKGSGVIFERAAGKLGWNPF
                     PAPMAILSQPRAGRSACVNCGFCLGFGCEVKAKSSSLASMIPLAEKSGKCEIRPNSYV
                     HKIEVDEHGRATGALYFDEKKNVHLQKAKAVILSANGAETPRLLLLSASSKFPNGLAN
                     SSGLVGKNLMFNTGAVSVGVMDEPLNDYKGYAVSRVLHDFYELDTSKVGFYGGGGLDA
                     RYDVTPIGFAFGGLPPGSPRWGKDYKALLAHNFPRSFEIFGHGTSLAVEDNSFSIDPD
                     MKDAWGLPALRLTYKDHPDDVKLMHWLADRSNELLEAAGAKQHWTFPIDEQQFGVHLL
                     GTCRMGNDPKSSVVNADHQTHDVKNLFLCDGSSLVTSGRGQPTMTIMALAFRAADRIR
                     DLAKKGELDA"
BASE COUNT          535 a          764 c          798 g          560 t
ORIGIN      
        1 agaatggtgg aggcgacgat gctgcagttg tcaaaggttg ttttcccaca acttccgtat
       61 cttctgggcg atgctgcgac agccggggcg ggggtcaact ttcctctact ttgaacagtg
      121 ggccacgcac acctcaccgc ctctacgcga aagagattta cctgtcggct accagctatc
      181 ggttgtcagc tgccgccctt tactgcagcg tggagttgtc gatgacgaag cggtatttca
      241 cgtcctgctt cagcagccgg tcatacgctt cattgatttg ctggatgcgg atcttttcaa
      301 tgtccgcggt aatgccgtgc tcgccgcaga aatcgagcat ttcctgcgtc tcgcggaggc
      361 cgccgatcgc ggatccggaa aggctgcgcc ggccgaacag cagattgaac gcattcgccg
      421 gcaggggctt ttccggcgcg cccaccaggc aaaccgtgcc gtctcgccgc aacaggttca
      481 ggtaggcgtt gatatcgtgc tgagccgaaa cgcaatccag aatgaagttg aaagagccgg
      541 cgtgcttctg catcgcgtcc gcgtctttcg agatcaccac ctcgtcggcg cctaaccgct
      601 tggcgtccgc ggttttaccg gccgaagttg tgaagagcac cgtgtgtgcg ccaagggcgt
      661 gcgcgaactt cacgcccatg tgacccaggc cgccgagtcc gacgatgccg accttctgcc
      721 ccttgccgac cttccagtga tgcagcggcg aaaacgtcgt gatacccgcg cagagcagcg
      781 gcgctgtacc cgcgggatcg aggccgttcg gcacgcgcag cacaaagctt tcgtctacca
      841 cgatgctgtc ggaatacccg ccgtaggtaa cgccgccgag gattttgtcc tcgctgttgt
      901 acgtcagcgt aaaagtattt tcgcaatact gctcttcacc ggccttgcag ctcgagcaaa
      961 ttcggcagga atccaccaga cagcccacgg ccgcaatgtc gccttctttg aacttcttca
     1021 ccgcgctgcc gaccttggtc actcggccca cgatttcatg gcccggcaca cagggataca
     1081 ccgtcggcat tacttctttc cattcgttgc gaacctggtg cagatcggag tggcatacgc
     1141 cgcaaaacag aatttcgagt tgtacgtcct gcggtcgcgg tgcgcgacgg gcgatcgtat
     1201 gcggagcaag cgcgccggta ggacttggcg cggcaaatgc cttggctgga gatgtggtgg
     1261 gagaggtacg ggcagcttca ctcatgggat gcctcctacg gcaataaagt aattcggggc
     1321 ggcgcgaact gagcagccgg aaagtaattt agactcgcgt tgctgcacaa tggttataag
     1381 gaacttgcac aattcgagat ccgcatcgtt accgccgcat tgtaacgtta cagcaatatc
     1441 aggaatatca ggcgtccagc tcgccttttt tggccaggtc gcgaatgcga tcggcggcgc
     1501 gaaacgccaa cgccatgata gtcatggtcg gttggccgcg gccggaagtg accaggctgc
     1561 tgccgtcgca aaggaacagg tttttcacgt cgtgagtctg gtggtcggcg ttgacgaccg
     1621 aactcttggg gtcattgccc atgcggcagg tgccgagcag gtgtacgccg aactgctgct
     1681 cgtcgatcgg gaaggtccaa tgttgtttgg cgcctgccgc ctcgagcaac tcgttcgagc
     1741 ggtcagccag ccagtgcatg agtttgacat cgtcgggatg gtccttatac gtgaggcgca
     1801 gcgccggcag tccccacgcg tccttcatgt cgggatcgat cgaaaagctg ttgtcctcta
     1861 ccgccagcga agtgccgtgg ccgaaaattt caaacgagcg agggaagttg tgtgccagca
     1921 gcgctttata gtctttgccc cagcgcggac ttcctggcgg caatcctccg aatgcgaagc
     1981 caatcggcgt gacgtcataa cgggcgtcga gaccgccgcc gccataaaag cccaccttgc
     2041 tcgtatcgag ttcataaaaa tcgtgaagga cgcgacttac tgcatagccc ttgtaatcgt
     2101 tgagcggttc atccatcacg ccaaccgaaa ccgcaccggt gttgaacatc aaattcttgc
     2161 cgacaaggcc gctggaattg gcgagaccgt tggggaactt actggacgcg gaaagcagca
     2221 acaggcgcgg agtttcggca ccattcgcgg agagaatcac cgccttggct ttttgcaaat
     2281 ggacgttttt cttttcgtcg aaatagagag cgccggttgc gcggccgtgc tcgtccactt
     2341 caattttgtg aacgtagctg ttgggccgga tttcgcactt acccgatttt tccgccaggg
     2401 gaatcatgct ggcgagcgaa ctggatttgg ctttcacttc gcagccaaag ccgaggcaga
     2461 agccgcagtt cacgcaggcg ctgcggccgg cgcgaggctg ggagagaatc gccatcggcg
     2521 ctggaaacgg gttccaaccg agcttgccag cggcgcgttc aaagataacg cccgaacctt
     2581 tcacaggcag cggtggcatg ggatagggct tggagcgtgg cggatcgaac ggactcgcgc
     2641 cggcgaggcc ggagacg
//
LOCUS       DASTPM010000778         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_893307, whole genome shotgun sequence.
ACCESSION   DASTPM010000778 DASTPM010000000
VERSION     DASTPM010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893307"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VFR42_13730"
     CDS             <1..1147
                     /locus_tag="VFR42_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU0370273.1"
                     /translation="AGAILAAAFIVISTTRSHKETLQLRAEMARLSTGLGLREQLVQR
                     TEQARSSLDASAKSDSAVRDTLRAQSEDLRKKMAGADGADAQSLKQQLADTQKRLKSL
                     ENEGKIAETIVHSYGPSVCLLHVVVEFLDKDSGKPIQIALDGLGKPQVDDKGMVRLDA
                     GGNGPHLQIDVFGTGFLVRRDGRILTNHHVAEPWWSNDELKELLDHGATGYVLSYKAY
                     FPGSSEGISAKLDKISSSADVATLKLEAATPPRATLLELDDRSDASVSGEPVVLIGYP
                     TGIEGILARAGSDVTQKIADGTQNVNQIVSQLAEQKLIRPTTTQGHIGDVLKDKIVYD
                     AATTSGGSGGPLFNRDGKVIGVNFAILKGFGGSNLAVPARYAQELLK"
     gene            1295..>2018
                     /locus_tag="VFR42_13735"
     CDS             1295..>2018
                     /locus_tag="VFR42_13735"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0370274.1"
                     /translation="MTKPEVHTESAEARLGKRAHLRRKLAPMAYVELGQDNGGILLNL
                     SEGGFAVQSALTLTSREFPELRFQVPAHQGWLTARGRIVWLSASKKEAGVQFTELPGE
                     ARQEIYKWVSTDGEPEKPTERFPKPPRETRPASRPIIETPSRAGSDARETQVAYNAVQ
                     NRERARSPQTEPAPVAVAEPPAQDFRFTDYSMFAEVPEKDAVWAEPARRRGSWGVRAL
                     LVILVAALFFALGATVGRSTVDE"
BASE COUNT          447 a          643 c          591 g          337 t
ORIGIN      
        1 cgccggcgcc attctggccg cggcgtttat cgttatttcg accacccgca gccacaagga
       61 aaccctgcaa cttcgcgcgg aaatggcgcg gctcagcacc ggactcggct tgcgcgagca
      121 actcgtgcaa cgcaccgagc aggcccgcag ttcgctcgac gccagcgcca aatccgattc
      181 cgccgtgcgc gatacgctgc gcgctcagtc ggaagattta cgcaagaaaa tggcgggcgc
      241 ggacggcgcc gatgcccagt cgctcaaaca acagctggcg gacacccaga agcgcctgaa
      301 atccctcgaa aatgaaggca agatcgcgga gacaattgtg cacagctatg ggcccagcgt
      361 ctgcctgctg cacgtagtgg tggaattcct cgacaaagac agcggcaagc caattcagat
      421 cgcattggac ggcctgggca aaccgcaagt ggatgacaaa ggcatggtgc gcctggacgc
      481 cggcggcaac ggcccccatt tgcagattga tgtgtttggc accggttttc tcgtgcgccg
      541 cgacgggcgc atcctgacca atcatcacgt ggccgaaccg tggtggagca acgatgagct
      601 gaaagaattg ctcgaccacg gcgcgacagg ctatgtgctc tcctacaaag cgtacttccc
      661 cggaagctcc gaaggaatat cggcgaagct ggacaaaata tcctccagcg cggatgtggc
      721 cacgctgaaa ctggaagcgg ccacaccgcc gcgcgcaacc ttgctggaat tggacgaccg
      781 cagcgacgcc agcgtatccg gcgagccggt agttctgatc ggatatccca ccggcatcga
      841 aggcattttg gctcgcgccg gttccgacgt gacccagaaa atcgccgacg gcacccagaa
      901 cgtgaaccag atcgtctcgc aactggccga gcaaaaattg atacgcccga ccaccacgca
      961 aggccacatc ggcgacgtgc tgaaggacaa aatcgtctac gacgccgcta ccacttcggg
     1021 cggctccggc ggcccgctgt ttaatcgcga tggcaaggta atcggtgtga atttcgctat
     1081 attgaaggga ttcggcggct ccaacctcgc cgtccccgcg cgctacgccc aggagttact
     1141 caaataggtg gccaggctac cgcattcccc cgcttgaagc acccctcccg agggcgtaga
     1201 atggtacttg gtagtaggtc gtcttttgca agcctaggta cgccaagctg ttgatacagt
     1261 tgcgctccac ccacccggcg cgacaggtac aaccatgact aaaccggaag tacacaccga
     1321 gagcgccgaa gcacgcttag gcaagcgcgc ccacttgcgc cgcaaactag cgcctatggc
     1381 ctacgttgaa ttgggccagg ataacggcgg catcttgttg aacttgagcg aaggcggctt
     1441 cgccgttcag tcggcgctga ccttaacctc gcgcgaattc cccgagctgc gatttcaagt
     1501 ccccgcacat cagggctggc tgaccgcccg cggccgcatc gtttggctca gcgcctccaa
     1561 aaaagaagcg ggagttcagt tcaccgaact gcccggcgaa gcccgccagg aaatctacaa
     1621 gtgggtttcc acggacggcg aaccggaaaa accaacggaa cggtttccca aacctccccg
     1681 cgaaacgcgc ccagcttcca gaccgattat cgagacaccc agtcgcgccg gtagtgacgc
     1741 tcgcgaaacc caagttgcgt acaacgcggt ccagaacaga gagcgcgcac gctcgccgca
     1801 aaccgagcct gcccccgttg cggtggccga gccgccggcc caggattttc gattcaccga
     1861 ctactcgatg ttcgcggaag ttccagagaa agacgccgtg tgggccgagc cggcacgccg
     1921 cagaggcagc tggggcgttc gggcgctatt ggtgattttg gtagcagcac tcttctttgc
     1981 gctgggcgcc accgtggggc gcagcaccgt agacgagt
//
LOCUS       DASTPM010000779         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_576572, whole genome shotgun sequence.
ACCESSION   DASTPM010000779 DASTPM010000000
VERSION     DASTPM010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576572"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFR42_13740"
     CDS             complement(<1..348)
                     /locus_tag="VFR42_13740"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HEU0370275.1"
                     /translation="MKYDSAKVLLVADLAGTFLFGIEGADTAIRGHLDLLGLMVLAFS
                     TALAGGVIRDLLIGAVPPASIKSWRYATTAFVAAILTFFFYRMVEAFPARLLMVLDAG
                     GLALFAVAGTDKAL"
     gene            475..666
                     /locus_tag="VFR42_13745"
     CDS             475..666
                     /locus_tag="VFR42_13745"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HEU0370276.1"
                     /translation="MAKVLRCRDLGVDCDFIARGATSEAVLEKAKDHACSDHGFASIP
                     PELADKVVAAIRDEEAATA"
     gene            799..1794
                     /locus_tag="VFR42_13750"
     CDS             799..1794
                     /locus_tag="VFR42_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0370277.1"
                     /translation="MQKRKLGKSDLEVSALGLGCMGMSFGYGPPADKTEMISLIRTAV
                     ERGVTFFDTAEVYGPFSNEELVGEALSPLRNQVVIATKFGFKPDPNGGQRWSALDSRP
                     EHIKQVADASLKRLGTDVIDLFYQHRVDPDVPIEDVAGAVKDLIQQGKVKHFGLSEAG
                     VATIRRAHAVHPVAALQSEYSLWWREPEAEVIPTLEELGIGFVPFSPLGKGFLTGKIS
                     EGTQFDKTDFRNVVPRFTAENRRTNQAMVDLIAKFAQQKKGTPAQIALAWLLAQKPWI
                     VPIPGTTKLHRLEENIGATAIELSPDELRELENAAAKIAVQGARYPEALQKLVGR"
     gene            1998..2393
                     /locus_tag="VFR42_13755"
     CDS             1998..2393
                     /locus_tag="VFR42_13755"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0370278.1"
                     /translation="MEIKRAGSQPSAKGPSEWFTGAVRIDPLFQAPDPALVQGASVTF
                     EPGARTAWHTHPLGQTLIVTAGCGWAQRQGGPVEEIRPGDVVWFPPNEKHWHGATPTT
                     AVTHIAIQEALNGKVVDWLEHVPDQQYRR"
BASE COUNT          612 a          826 c          659 g          479 t
ORIGIN      
        1 cagcgccttg tccgttcccg ccaccgcaaa caacgccagc ccacccgcat ccagcaccat
       61 cagtaaccgt gccggaaacg cttccaccat ccggtaaaag aaaaacgtca gtatcgccgc
      121 cacaaaagcc gtagtcgcat atcgccagct cttgatcgac gccggcggga ccgctccgat
      181 cagcaaatcg cgtatcactc cgcccgccag cgccgtagaa aacgccagca ccatcagacc
      241 cagcaaatcc aggtgcccgc gtatcgccgt gtccgctccc tcaatgccaa ataaaaacgt
      301 ccccgctaga tccgctacca acaacacttt cgcagaatcg tatttcatgc ccaaagccta
      361 acaaattcca gtggactgcc gggacggcat acctcctgca actcaagtcc gtttgactgc
      421 cgcagaatat gcaactacta tccgccggcc ctgtgctggc ggacgagggc cgcaatggca
      481 aaagttctca ggtgtcgaga ccttggtgtg gactgtgatt tcatcgcccg tggcgcaacc
      541 agcgaagcag tcctcgaaaa agccaaggac cacgcttgct ccgatcacgg ctttgcaagc
      601 attcctccgg aattggccga caaggtagtc gcagccatcc gcgacgaaga agccgccacc
      661 gcctaaaact gcatgccgca aaaagacccg attgcgcggc cggctttggc cgcatttggc
      721 cgttgacgcc cctcgcccgc agacctactt tctccccatg tgaggcacac cacggaacag
      781 cttcagaagg agaaaacaat gcaaaaacgc aaacttggca aaagcgatct cgaagtctcc
      841 gctctcggcc tcggctgcat gggcatgagc ttcggctacg gcccgcccgc cgacaaaaca
      901 gaaatgattt cgctgattcg caccgctgtc gaacgcggcg tcaccttctt cgataccgct
      961 gaagtttatg gtcccttcag caacgaagaa cttgtgggcg aagcattgtc cccgcttcgg
     1021 aatcaggtgg tgatcgccac gaaattcggc ttcaagcctg accccaatgg tggacagcgt
     1081 tggagtgctc tcgatagtcg cccagaacac atcaagcagg tcgccgatgc ctcgctcaaa
     1141 cgcctcggga ccgacgttat cgacctgttc tatcaacacc gtgtcgatcc agacgttccg
     1201 atcgaagacg tagccggtgc ggtgaaggat ctgattcagc aaggcaaagt gaagcacttc
     1261 ggactttccg aagcaggcgt tgcgaccatc cgtcgcgcac atgccgttca cccggtcgcg
     1321 gcgctgcaaa gcgaatattc tctctggtgg agggagccgg aagcggaagt gattcccacg
     1381 ctcgaggaac tcggcatcgg cttcgttccg tttagtccgc tgggaaaagg cttcctcacc
     1441 ggcaagatca gcgaaggcac acagttcgac aagaccgact ttcgcaacgt ggttcctcgc
     1501 tttacggcgg aaaaccgcag aacgaatcag gcgatggtcg atctgatcgc aaagttcgca
     1561 cagcagaaga agggcacgcc ggcgcagatc gcgcttgcct ggctgctcgc ccagaagcct
     1621 tggatcgttc caattccggg taccaccaag ctgcatcgcc tcgaagaaaa tatcggagcc
     1681 actgccatcg aactctcgcc cgacgaactc cgcgaattgg agaacgcagc tgcaaagatt
     1741 gccgtacaag gcgcccgcta cccggaagca cttcagaaat tggtgggccg ctaacttttc
     1801 tatctggagc tcatttgacg gtctatctcg cagcagccgg aaaaccagct gtaaggtaaa
     1861 aactgtcgcc gaacttgaaa aaactattta cgcttggcgt agtggtctgg ttgatcggta
     1921 tcgccggcaa ctgtcaaaat tacgccgcgc gagtccgagg cctgtcctgc gggactagga
     1981 tgcgaggaat cgcaagaatg gaaatcaaaa gagccggttc gcaaccctcc gccaagggac
     2041 catctgaatg gttcactggc gccgtgcgca tcgatccgct gtttcaagct cccgacccgg
     2101 ccttggttca aggcgccagc gtcaccttcg aacccggcgc ccgtaccgca tggcacaccc
     2161 atccgctcgg tcaaacgctc atcgtcactg ccggttgcgg ctgggctcaa cgccaaggcg
     2221 gtcccgtcga agaaatccgc cccggtgacg tagtctggtt ccctcccaac gaaaaacatt
     2281 ggcacggcgc cactcccacc accgcggtga cccacatcgc cattcaggaa gcgcttaacg
     2341 gcaaagtcgt cgactggctc gaacacgtcc ccgaccagca ataccgcagg taatcgcccc
     2401 tcacgacagc ggttctgaag ccgatccgct tgaaccaact ccgagcagac tcaaattccg
     2461 tcttctcaac tccgcccatt tgtgatagct ttcactttgc aagtgaagtg atgctcgccg
     2521 ctcaacgcca aaccgtcggg ttcggaattc cggccttagg agaagtcatg aagcaa
//
LOCUS       DASTPM010000780         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_835554, whole genome shotgun sequence.
ACCESSION   DASTPM010000780 DASTPM010000000
VERSION     DASTPM010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835554"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1666)
                     /locus_tag="VFR42_13760"
     CDS             complement(179..1666)
                     /locus_tag="VFR42_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU0370279.1"
                     /translation="MNNRFLLWAAACLTLVSCAIRQAPVGEEIMPESARRQIPGHWAG
                     PHRSGTVVPNWILSFHDPELTALVADAVERNPDLKAAAARVEASRAAVRIAASSLYPR
                     IAMKGLGERQGQHLNGDLGRGINPPDFGSSGTENAGGAGLDTSVDQTSQRWVYGIAAG
                     AAWEADVWGRIRSKKAAAKAESQALEADYEFARQSLAAAVARAYFTTIEAAQQEANAQ
                     DTLNIYEEYSKLTDIQKQHGFASDFDVSQIKSRTATAEDALYTAQAVRAQTIRAIEVV
                     TSHYPAGRFDVRRSFPGQPRAAPAGLPAQILERRPDLIASERRFAAAFHRVNEARTAR
                     LPRFVLSAGSGLGTADLDSVGSLNAVLWSFAGGITQPIFFGGELQAAQDLRTAEQKAA
                     AASYVGHALRSFGEVEDALANDYYLRKREGALADAVSSSADSIKFGRQQLEQGQIDMF
                     TVLRLTGENLAAKVQYTQIRASRLRERVNLYLALGGDFGGTGAPK"
     gene            complement(1677..>2022)
                     /locus_tag="VFR42_13765"
     CDS             complement(1677..>2022)
                     /locus_tag="VFR42_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370280.1"
                     /translation="NELRYVEPGNEAEIFLETYPGRIIKCKVDSIMWATAQGQMPISG
                     WVGTTAGLGMLSDRIAVRLLVEPKDRQLFLAAGAVGGGAIYTEHGKMIHIVRKVFVRV
                     SAKLDWFVFKLH"
BASE COUNT          383 a          610 c          593 g          436 t
ORIGIN      
        1 tgcaacggtt gcttgtccca actcatgcgc gtcatgctca agcagtatat gagttcgtag
       61 cgcctcagtt gcagatcgga agtggataaa tgttgaattt cattctgcgt cgaacaacag
      121 cctgccataa caagcccggc gattgttatt agtagtatct gtttcactct cgattttctc
      181 attttggcgc gcccgttccc ccgaaatcac cgcctaacgc caggtagaga ttcacgcgtt
      241 cgcgaagacg cgaggcgcgg atttgtgtgt attggacttt cgcggcgagg ttttcaccgg
      301 tgagacgcaa cacggtgaac atgtcgatct gaccctgctc caactgttgc ctaccgaatt
      361 ttatcgagtc ggcgctgctg gaaactgcgt cagccagcgc gccttcgcgt ttccgcagat
      421 agtaatcgtt cgcgagagcg tcttcgacct ctccgaacga gcgtaaggca tggcccacgt
      481 aactcgcggc agccgctttt tgctcggcag tgcggaggtc ttgcgcggct tgaagttcac
      541 cgccgaaaaa aatcggttga gtgatcccgc cggcgaaact ccagaggacg gcattgagcg
      601 acccgactga gtcgaggtcc gcggttccaa gtccactacc ggcgctaagc acaaagcgcg
      661 gcaggcgtgc ggtgcgagcc tcgttcaccc gatgaaatgc agcggcgaag cggcgctcgc
      721 ttgcaatgag atccggccgg cgttcgagaa tttgcgcagg cagacctgcg ggcgcggctc
      781 tcggctggcc ggggaacgac cgtcgcacgt caaaacggcc agccgggtaa tggctggtga
      841 caacttcgat cgcgcggatc gtctgcgctc gcactgcttg cgcagtataa agcgcatcct
      901 ctgcggtggc ggtgcgcgac ttgatctgcg atacgtcaaa gtcgctcgcg aagccgtgct
      961 gcttctgtat atccgtgagt tttgaatatt cctcgtagat gttgagcgta tcctgcgcat
     1021 tcgcttcctg ttgcgctgcc tcgatcgtag tgaagtacgc gcgcgccacc gcggcagcga
     1081 gcgattgccg ggcgaattcg tagtcggctt ccaaggcctg gctttcagcc ttcgcggcag
     1141 ccttttttga acgaatgcgg ccccacacgt cggcctccca tgccgcacca gcagcgatgc
     1201 cgtacaccca gcgctgtgat gtctgatcga cgctcgtgtc gaggccggcg ccgccagcgt
     1261 tctccgttcc tgagctcccg aagtctggag gattgatgcc ccgaccaagg tcaccattaa
     1321 gatgctgtcc ctggcgctca cccagtcctt tcatcgcgat gcgtggataa agcgatgatg
     1381 cagcgatgcg cacggcagca cgcgatgctt cgacccgcgc ggctgcggcc ttgagatcgg
     1441 gattcctctc gacggcatcc gcaactagcg ctgtcagctc cggatcgtgg aagctgagaa
     1501 tccagtttgg tactacagtg ccgctgcgat gcgggcctgc ccagtggccc gggatttgcc
     1561 tgcgggccga ttcgggcatg atctcttcgc caacaggtgc ctgtctgatc gcgcaactta
     1621 caagcgtaag gcacgctgcg gcccacagaa gaaatcggtt attcatgcag accgctttag
     1681 tggagcttga aaacaaacca atcaagcttc gctgagacac gaacgaagac tttgcggacg
     1741 atatggatca tcttgccgtg ctcggtgtag atcgcgccgc ctcccaccgc gccggctgcg
     1801 aggaagagtt gcctgtcctt cggttcgacc agaagccgga ccgcgatgcg gtccgagagc
     1861 atgccaagcc ccgctgtggt ccccacccaa ccgctgatgg gcatctggcc ctgtgccgtg
     1921 gcccacatga tagagtccac cttgcacttg atgatccggc ccgggtaggt ctcgaggaag
     1981 atctccgcct cgttgcctgg ttcgacatac cgcagctcgt tc
//
LOCUS       DASTPM010000781         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_777244, whole genome shotgun sequence.
ACCESSION   DASTPM010000781 DASTPM010000000
VERSION     DASTPM010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777244"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..235)
                     /locus_tag="VFR42_13770"
     CDS             complement(98..235)
                     /locus_tag="VFR42_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370281.1"
                     /translation="MIRTAIISVTASVVLFGAWTINLVSYHPANDLNPLAPLSAPVDW
                     K"
     gene            complement(775..1146)
                     /locus_tag="VFR42_13775"
     CDS             complement(775..1146)
                     /locus_tag="VFR42_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU0370282.1"
                     /translation="MSDDVVGFGFLAESDIPVWYLKAGETIFKEGETGKELYVIKSGQ
                     VEIRLENRLLATLEANDIFGEMALFDSTPRSATAVAKTDVALVPVSKKDFVALVSRAP
                     TFALDIMSMLARRLREANKAI"
     gene            complement(1165..1845)
                     /locus_tag="VFR42_13780"
     CDS             complement(1165..1845)
                     /locus_tag="VFR42_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0370283.1"
                     /translation="MAPSSRPTDLLSDLPEQLSSGLLANAKPVKLAANEILFLAGDPG
                     EGCFRLNEGLLKVSMVSPTGAERILAILGPGSIVGDMAMIDGRPRSASVSALRDCKLS
                     FVSRSAFEAVAEKNPEIYKHLLSLLAARLRDTDQVVAAGTFLPVKGRVARALLDLAKA
                     FGNDVGGGRVVIRQKLSQSDVAAMAGIARENVSRILNEWMRLKLVTRLSGYYCIENKS
                     RLEKELKL"
     gene            complement(1963..2811)
                     /locus_tag="VFR42_13785"
     CDS             complement(1963..2811)
                     /locus_tag="VFR42_13785"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0370284.1"
                     /translation="MRHLPLFVCLCSLTIFGTCTVRGEETRDQAPAPAAAAESATNAG
                     EQSVPPAAAAKPVIERSKREICDTLVQSAQSNALPIPFFIRLLLQESGFRSDVVSRAG
                     AQGIAQFVPETARSVGLDNPFDPLQAIAASARLLRNLVTKFGNLGLAAAAYNAGPRRI
                     SNWLVRKSKLPQETQHYVKITGTSGGNVEGQTGADCRTEKHAPCRDDAGVIASETPSA
                     PQIEARSRVLARSSAKETSRTTVAQASANPARTSLARLAKRHKGGKGAVQLAAASKKS
                     RHKAQS"
BASE COUNT          602 a          901 c          888 g          599 t
ORIGIN      
        1 taatacggac cgcagtccgg ctattgcaac gatgcagttg gcagttccct gaggaacggc
       61 gttgcgagtt cgtacgcatg aaacggcgac ccagcgtcta cttccagtca actggagcgc
      121 ttaacggagc cagcggattc agatcgttcg ccggatgata gctgaccaga ttgatggtcc
      181 atgcgccgaa cagaacaacc gacgccgtaa cggaaataat cgcggtacgg atcatggcgg
      241 agctccctgc ccgggatttt tcccggtgct gccggcacat ttccgctgcc ggcagcggtg
      301 gagttagaag aactcccgag ccgcggctgt gacccgcatc acaatcgacg cctgcctgtt
      361 tgctattccc cgtccaggtt ttgccaagaa aaaagcggcc cgtattggcc gcttgaagtt
      421 tggaggttga aatctgggcc tgccccggga cgggggcagt ccgaacggcg gtcaggttgc
      481 ggggaaaacc ttcgccgcca gggtgccgat ggtaagaggg tcaaccacca gacgggtcga
      541 actcaattcg atcacaggtc catgaacgct ccgggccaaa ccggtgacca ccgacactcc
      601 agcgagaacg cggtagcaat ggcgaggatg atggccttgc gcatggcgtg ctctgaagcg
      661 ggagcaaccg cgctccctac gctcacaaca ctaggccgcc gggatctgcg gcgatgtgga
      721 cccccatcac ataaacgcgc ttttggaagc gcctcttttg gactgctgct tgatttatat
      781 cgccttattg gcttcgcgca gccgccgcgc cagcatgctc atgatatcca gggcgaaagt
      841 gggggcccgg ctcaccaagg ccacgaaatc ctttttcgag acggggacca gcgccacatc
      901 ggtcttggcg acggccgtcg cgctgcgggg cgtcgaatca aacagtgcca tttcgccgaa
      961 aatgtcatta gcttcgaggg tggcgagcag gcgattctcg agccgtatct cgacctgtcc
     1021 gctcttgatg acgtaaagct ccttccccgt ttctccctct ttaaagatcg tctcgcccgc
     1081 tttcaggtac cataccggaa tgtccgattc ggcgagaaag ccgaaaccca cgacgtcatc
     1141 cgacatccgg cttgcccctg aacactaaag tttcagctcc ttctcgagcc gcgatttgtt
     1201 ctcgatgcaa tagtaacccg atagccgggt gaccaatttc aaacgcatcc attcgttcag
     1261 gatgcggctg acattttcac gtgcaatgcc agccattgcg gctacgtcgc tctggctcag
     1321 tttctgcctg atgacaacgc ggccgccccc gacgtcattg ccgaatgcct tggccaagtc
     1381 gagcagcgcg cgtgcaacgc gtcctttgac cggcagaaac gttccggccg ctaccacctg
     1441 atcggtgtct cgcaaacggg cggcgagcag gctgagaagg tgcttgtaaa tttcgggatt
     1501 cttctccgct acagcttcga atgcggaacg gctaacgaaa ctcagtttgc agtcgcgcag
     1561 cgccgaaacc gaagccgagc gtggtcggcc atcgatcatc gccatgtcgc cgacgatcga
     1621 accaggaccg agaatcgcga gaatgcgctc ggcaccggtg ggcgacacca tgctcacttt
     1681 cagcaagcct tcattgagac ggaagcagcc ctctccgggg tcgccagcaa gaaaaaggat
     1741 ttcgttggcg gccagtttga ccggtttcgc gtttgcgagc agcccggacg acagctgctc
     1801 cggcagatcc gacaacagat cggtgggacg cgaggacgga gccatcagac acccgacgcg
     1861 aactaaacat gacgcgccgg tcttttagca gaaattgcat caaaaagggc atcggtgggg
     1921 tcccaaccca tgggaaccga ggcctgaagg cggccggtac tactatgact gcgctttgtg
     1981 ccggctcttt ttgctggctg ctgccagctg gacagcccct tttccacctt tgtggcgctt
     2041 tgcaaggcgg gccaacgacg tcctggccgg attggctgat gcttgagcaa ccgtagtccg
     2101 ggatgtttcc ttcgcgctgg aacgcgccag gacgcgcgat cgggcctcga tttggggcgc
     2161 gcttggcgtc tccgaggcaa taactcccgc gtcgtctcgg cacggagcgt gcttctctgt
     2221 tcggcagtcg gcgcccgttt gcccttccac gtttccgccg gacgtccctg taatcttcac
     2281 gtaatgctgc gtctcttgcg gcaatttgct cttcctgacc agccaattgc tgatgcggcg
     2341 cgggccggca ttgtatgcgg cggcggccaa accgagattg ccgaacttcg tcacaaggtt
     2401 gcgcaacaac cgtgccgagg cagcgatggc ctgcagaggg tcgaacggat tgtcgaggcc
     2461 cacggatctc gccgtctctg gcacaaactg cgcgatccct tgcgcgccgg cgcggctcac
     2521 cacgtcggac ctgaacccgc tttcctgcag cagcaggcgg atgaaaaagg gaatcggcaa
     2581 ggcgttcgat tgcgccgatt gcacgagcgt gtcgcagatt tcacgctttg agcgttcaat
     2641 gaccggcttc gccgccgccg ctggcggaac agattgttcc ccggcgttcg tggcactctc
     2701 ggcagccgcg gctggcgctg gagcctggtc gcgtgtctcc tcgccgcgca cggtgcaggt
     2761 gccgaagatc gtgagcgagc aaagacaaac gaacaaaggc aagtggcgca tgtcgtcctc
     2821 tcgaatgcgg acgcacgcgc aggcacgcat cgtcgcaaat attgaatgta aaaggtggga
     2881 gctccccccg tccgcgggct aagtgcattc gaatcgggac agaattcggg aatgcttgtg
     2941 aaaaaattaa gaattggaga cttttggcat atgcggttcc ccgcgtgacc
//
LOCUS       DASTPM010000782         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1383547, whole genome shotgun sequence.
ACCESSION   DASTPM010000782 DASTPM010000000
VERSION     DASTPM010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383547"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1591
                     /locus_tag="VFR42_13790"
     CDS             125..1591
                     /locus_tag="VFR42_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU0370285.1"
                     /translation="MALVGNLEQARAVVRFAIALILSHGASLALAQRAPVSPDHPWHG
                     LGEARIEADARNFTEPGLSFDREKIYSLPELIDLAESHNPETRVAWERARAQAAAWGV
                     ARSELYPTVAAAALAGVDRGQVYLADRFFRQTIGDFEVVLNLNYTIFDFGARAGRISA
                     AKAEALAANFAFNDTHREVIYRVEQAYYQLLNAAGQEEAARANLSNAQAVQQAAEERL
                     NDGLATLPDVLEARSATAQAEYELQAVLGAEEIAHGNLATALGTVPQTAIHVQPLREL
                     TIPSTIADTVNSAMDRALAQRPDLLQRVADVQAARARVKEARAAYYPRLDFTASPTAQ
                     SLYGLQQQFPWGQTAGLAGGMAFKLNWTLFDGGARKNKLAQAEANVHAAEAQIHASRD
                     QIANEVWAAYSDLNTALRQREAAAALLEATGRSYDAALESYNYGLRNLLDVTAAQRTL
                     AQARSTDVLARTQVLTTLAALAFRAGDSIQSNAMKPRP"
     gene            1588..1860
                     /locus_tag="VFR42_13795"
     CDS             1588..1860
                     /locus_tag="VFR42_13795"
                     /inference="COORDINATES: protein motif:HMM:NF028400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HEU0370286.1"
                     /translation="MTLVRDSRLVRLLPLLLVAPLLPGCSRAPSVEVLGSFFPAWLVC
                     FIVAIVLTALVRLALLRLRMKAALPLLVYPSLAALFTFLLWLIFFY"
     gene            1915..>2386
                     /locus_tag="VFR42_13800"
     CDS             1915..>2386
                     /locus_tag="VFR42_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU0370287.1"
                     /translation="MELDSAATTTRQLSRWVSIGILLAAIVLGLAVLYHINHSPRTDD
                     AEVSANFIGIAPQVEGPIVRLNVRDNQFVKKGELLYEIDERPYQYAVERALSEQAALE
                     GQIADEQRKINALVSGVSVAKANISGSEADVARWAAAVEQARADVANAEQGVNRA"
BASE COUNT          488 a          712 c          723 g          463 t
ORIGIN      
        1 gtgccggtgc cgccgctgac gctggacacc gaaattcaaa aaaagcgttt ccacgaagcc
       61 gagatacgca agcagatgcg gctggaagcg gcgctcgaga aggaagaagc ctgagccggg
      121 gaccatggca cttgttggca atctcgagca ggcgcgggcg gtagttcgtt ttgcgatcgc
      181 actcattctt tcgcacggcg caagtctcgc tctcgcgcag cgtgcacctg tgtcgcctga
      241 ccatccttgg catggacttg gggaagcgag gatcgaagcg gacgcgagaa atttcaccga
      301 acccggactc agcttcgatc gggagaagat ctactccttg ccggagctga tcgatctggc
      361 cgaatcgcac aatccggaga cgcgcgttgc ctgggaacgc gctcgcgcgc aagccgccgc
      421 gtggggtgtc gcgcgcagcg agctctatcc caccgtagcg gcagcggcac tggctggcgt
      481 tgaccgcggg caagtttatc tggcggaccg tttctttcgg caaacgatcg gcgactttga
      541 ggtcgttttg aatctgaatt acacaatctt tgacttcggc gcgcgggccg gccgaatcag
      601 cgctgccaaa gccgaggccc tggcagcgaa cttcgccttc aatgacacac accgcgaagt
      661 gatttaccga gtggagcagg cctactacca actcttgaat gccgctggcc aggaagaagc
      721 cgcccgcgcg aatctttcga acgcgcaggc cgtgcagcaa gcggccgaag agcgtctgaa
      781 cgatggcttg gcgactctcc cggacgtgct cgaagcacgt agcgcaaccg cacaggcgga
      841 atacgaactg caggcagtgc tgggagccga ggagattgcg cacggaaacc tggccaccgc
      901 tctcggaacg gttccgcaaa ctgccataca tgtccagccg ctacgggagc tgaccattcc
      961 gagcaccatc gccgacaccg tcaactccgc gatggatcgc gcactggcgc agcgccctga
     1021 cctccttcag cgggtcgccg acgtccaggc agccagagcg agggtcaagg aagcgcgtgc
     1081 ggcctactat cccaggctcg atttcaccgc cagtccaacc gcgcagtcgc tgtacgggtt
     1141 gcaacagcag tttccctggg gacaaacggc aggcctggcg ggtggcatgg cctttaaatt
     1201 gaattggact ttgtttgatg gcggggcgcg aaagaataag ctcgcgcaag cggaagccaa
     1261 cgttcatgcg gcggaggcgc aaatccacgc ttcgcgcgat cagatcgcca acgaagtttg
     1321 ggcggcatac tccgatttga ataccgctct gcgccagcgc gaagccgcgg ctgcgctgct
     1381 cgaagccacg ggccgttcct acgatgccgc tctggaatcc tataactacg ggcttcgcaa
     1441 tctgctggac gtgaccgcag cacagagaac gctggcgcaa gctcgctcga ccgacgtcct
     1501 ggcccgcacg caggtcctta cgactctggc cgctttggct ttccgagcgg gggactcaat
     1561 tcagtcgaac gcaatgaaac ccagaccatg accctcgttc gagattcccg cttggtaaga
     1621 cttctgcccc tgctgctggt ggcgccgcta ctaccaggtt gctcgcgggc gccgtccgta
     1681 gaggtgctag gttcgttttt cccagcatgg ctggtgtgtt tcatcgtggc gattgtgctg
     1741 acggctctgg tccggctggc attgttgcgc ttgcggatga aggcggcatt gccgttgctg
     1801 gtttacccga gcctggctgc actcttcacc ttcctgctgt ggctcatctt tttttattag
     1861 ggacttctat ggaatttctt aagtcacaat ttttagcaac gaggcgcaaa gaccatggaa
     1921 ctcgattccg ccgcgacaac taccaggcaa cttagccgtt gggtgagcat cggcattcta
     1981 ctggccgcaa tcgtcctggg cctcgctgtg ctttaccaca ttaaccactc tccgcgaacc
     2041 gacgatgccg aagtatccgc caactttatc gggatcgctc cgcaagtcga aggtcccatt
     2101 gtccgcctga acgtgcggga caaccagttc gtcaagaagg gcgaactgct atacgagatt
     2161 gatgagcgac cgtatcagta cgcggtcgaa agggctcttt cagaacaggc cgccctcgaa
     2221 ggacaaattg ccgacgagca gcgaaaaatc aatgcactgg tgagcggcgt ctccgttgct
     2281 aaagcgaaca tttccggttc cgaagccgac gtggcacgct gggcggcggc cgttgaacaa
     2341 gcgcgggcgg atgtggccaa tgcggagcag ggggtgaacc gggcaa
//
LOCUS       DASTPM010000783         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_691646, whole genome shotgun sequence.
ACCESSION   DASTPM010000783 DASTPM010000000
VERSION     DASTPM010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691646"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFR42_13805"
     CDS             <1..198
                     /locus_tag="VFR42_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370288.1"
                     /translation="QSDKATEFGILSFVHDAHPATAKLLNDAVVRDGLADHGIGAMLG
                     AVQGQVNESRGVGDISEGLLA"
     gene            348..1700
                     /locus_tag="VFR42_13810"
     CDS             348..1700
                     /locus_tag="VFR42_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEU0370289.1"
                     /translation="MMGQHDRSEALFYYFRLEDQIPENHLLRLIGKHISFEFVRLQLK
                     DSYSATGRPSIDPELLLRILLIGYLYGISSERKLVEELRMHLAWRWFTGLGFDQELPH
                     HSTFSKNRHGRFQESKLFEQLFEQIVRQCVEVGLVQGQHLSVDGSFVEANAAKQSRIP
                     RQQLAEAAQVNQTVRQYLVELEQQNPVEEPVHQQDQVSTTDPDSTYATKGGTPARLGY
                     YDNYLVDNHSCVIVGVQATAARMSQETVAAQDMLTRFAAWQGREPESVAADTTYGNGE
                     FLQWLADRNVTAYMHTRDSALRKNNPGYGPERFTYQLESNSYRCPAGEQLNYVGLNVR
                     NRAHAYIGSAKRCGACSQKAQCTTGRYKYLAIHIHEPVRQRARELAQTPEFAKAQRQR
                     KKVEALFAQLKNQIGLRRLRLRRMKFVREQFWLAATVQNIKRLVRFLSRQATPLAVNN
                     "
BASE COUNT          469 a          555 c          575 g          408 t
ORIGIN      
        1 cagagcgaca aagcgaccga gttcggtatc ctcagctttg tacacgacgc ccatcccgcc
       61 accgccaagc ttctcaacga tgcggtagtg cgagatggtt tggccgatca tggaatcggg
      121 gcaatgttag gcgcggtgca agggcaagtc aatgaaagcc gaggagttgg cgatatctca
      181 gaaggattgc tggcgtaata tcgcgatttc gcttatcgac agggttgtga gcgcaatcgc
      241 ctgtgggtgt tgaaaaagtc actgagatta gcaggtgaga attagcgaaa gcatggtaag
      301 acctcaacat gctttgctgg atttccattt taggaggcct gctgatcatg atggggcaac
      361 acgaccgctc tgaagcgctg ttctactact tccgtctgga agatcagatt cctgaaaacc
      421 acctgctgcg gctgatcggt aagcacatca gctttgagtt cgtacggctg caactgaaag
      481 acagctacag tgcgacgggc cgcccctcca tcgatcccga acttctgctg cgcattctct
      541 tgatcggcta tttgtatggc atcagcagcg aacgcaaact ggtggaagag ttgcgcatgc
      601 atctggcgtg gcgttggttc accggactgg gtttcgatca ggagctcccg catcactcga
      661 cgttctcgaa aaaccggcac ggacggttcc aggaatcaaa gctgttcgag cagttgtttg
      721 agcagatcgt gcggcagtgt gtggaagtgg gattggtgca gggccagcac ctgtcggtcg
      781 atggcagttt cgtagaagcg aacgcggcga agcaaagccg cattccgcgc cagcagttag
      841 cggaagcggc gcaagtgaac caaaccgtac gccaatactt ggtggaactg gagcagcaga
      901 atccggtgga agagccggtg caccagcagg atcaggtatc gaccaccgat cccgattcca
      961 cctacgcgac caagggtggg actccagccc gactgggcta ttacgacaac tatctggtgg
     1021 acaaccacag ctgtgtgatc gtgggagtgc aggccacggc agcgcgcatg agccaggaga
     1081 cggtggcggc acaagatatg ctcacccgtt ttgccgcatg gcaaggacgg gagcccgaat
     1141 cggtagcggc cgacactacc tacgggaatg gagagttctt acagtggttg gcggatcgca
     1201 acgtcactgc ctacatgcac acccgcgaca gtgctctgag aaaaaacaat cccggctatg
     1261 gccctgagcg atttacttat cagctcgaga gtaacagcta ccgttgtcct gcgggagagc
     1321 aactgaacta cgtcggcttg aatgttcgca atcgagccca tgcttacatc ggcagcgcga
     1381 aacgctgcgg agcatgctca caaaaagcac aatgcacgac tggccgatac aagtatctcg
     1441 ccattcacat tcatgagccg gtacgacaac gcgcacggga gttggcgcag acccccgagt
     1501 tcgcgaaggc acaacgacaa agaaagaagg tggaagcgct gttcgcacaa ctcaagaacc
     1561 agatcgggtt gcgccgcctg cggctacgcc gcatgaagtt cgttcgggag cagttctggc
     1621 ttgcggcaac ggtgcagaac atcaagcgcc tggtgcggtt cctcagccgg caagcaacac
     1681 cgctggctgt caacaactag aatcccgaac ggaacccgcc agtccctaca tcaccccgca
     1741 acgagccctt caatcagtga ctttttcaac acccacaggc gttagcactc aaaaccccgt
     1801 tcagcttgcc tccctcagga gtacttctgc ttcctcgccg tttcggaggc ttttgctagg
     1861 agctttcgag cggtctggcg gacacacctg gccttgctcg aaacatggga cactcacgac
     1921 attttcgttg gcggcgtcca tgccctcgat tggtgcgcct tctgcatttc cggatttttt
     1981 cacatgggcg ctgtcgcaga gagcttc
//
LOCUS       DASTPM010000784         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_143698, whole genome shotgun sequence.
ACCESSION   DASTPM010000784 DASTPM010000000
VERSION     DASTPM010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143698"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1141)
                     /locus_tag="VFR42_13815"
     CDS             complement(164..1141)
                     /locus_tag="VFR42_13815"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEU0370290.1"
                     /translation="MGLAEGHQVGAALSLDNEKRAGRNLLEYIGNTPLIRIERVTRNL
                     PNLEILAKAEWFNPGGSVKDRAALAMIQGGLASGKLRPGKTILDATSGNTGIAYAMIG
                     AALGYPVKLFLPHSASEERKRILHAYGAELVLTPGDEGTDGAIRRVRELAAAEPERYF
                     YPDQYSNAANWQAHYRSTANEIWEQTGGRITHFVAALGTSGTFVGTARRLKELNPEIR
                     CVSLQPDGPWNGLEGWKHMPTALRPAIYDDTLADENLEISTEESYRMVKRLAREEGLL
                     VSPSAAAALLGCLAVAKTIPAGQTAVMVTVFADSAAKYLSERFWDEGGS"
     gene            complement(1265..2380)
                     /locus_tag="VFR42_13820"
     CDS             complement(1265..2380)
                     /locus_tag="VFR42_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEU0370291.1"
                     /translation="MKRFLLLLVLIVFAGAGLSGAETPQRWSEQQAKDWYAKQGWLVG
                     SNYLPATASNELEMWQAETFDPKRIDLELGWAETIGLNTMRVFLHDLVWQQDAKGLTQ
                     RIDQFLKIAKKHHIKPMFVLFDSCWDPAPRLGPQLPPRPGIHNSRWVQSPGAAVLEDP
                     AQYSRLEAYVKGVVKAFGKDDRVLTWDVWNEPDNTNHGSYGSQEPDDKVQRVETLLPQ
                     VFRWAREAGATQPLTSGVWKGDWSSPEKLGATEKIQLENSDVITFHNYDKPDEFEKRI
                     KWLQAYQRPLICTEYMARGNGSTFEGTLPIAKREHVGAINWGLVAGKTQTLLPWDSWE
                     HPYTDREPAVWFHEIFRTNGQPYRKEETELIRQLSKS"
     gene            2501..2944
                     /locus_tag="VFR42_13825"
     CDS             2501..2944
                     /locus_tag="VFR42_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370292.1"
                     /translation="MWNVEQRFAPKAIEGLAALGFSVGSTRGVVRVEKYGCGAEFRQG
                     TDSRYQLTVLPTIMVKGQFARLWDAGYQKFLLTDDGIKLPALAWHLQNLRKFNEELRT
                     ALGVPTFYNEALGSVSQVSVYDRVKGRAGDVPDETVGAHSPGSGH"
BASE COUNT          595 a         1025 c          744 g          680 t
ORIGIN      
        1 tccttcctca aacgccgaat cccgccccag cagcgcacca cagcattcat gcggaaacgt
       61 ctctttgccg tgttgtccga tgcgttctgc caagtcgtgg ctaatattca acggaagtcc
      121 gtccgccatt tctcttccag cttagtgacc gtaagtacag gccctaagac ccgccttcgt
      181 cccaaaaacg ttcgctcaaa tattttgccg cagaatccgc aaaaaccgta accatcaccg
      241 ctgtctggcc ggcgggaatt gtctttgcaa ccgcaaggca gccaagcaat gcagccgccg
      301 cactcgggct caccagcaac ccttcctcgc gcgcgagccg cttcaccatg cgatacgatt
      361 cctcggtcga aatttccagg ttctcatccg ccaatgtatc gtcgtaaatc gccgggcgca
      421 gcgccgtagg catgtgcttc cagccctcca gcccgttcca cggaccatcc ggctgcaagc
      481 tcacgcagcg aatctccgga ttcaactctt tcagccgccg cgccgttccc acaaacgtcc
      541 cgctggtgcc cagcgccgcc acgaagtgcg taatccgccc gccggtctgt tcccaaattt
      601 cgttcgccgt gctgcggtaa tgcgcctgcc agttcgccgc attgctgtat tggtcgggat
      661 aaaaatatct ttccggctcg gcagcagcca gttcgcgcac gcgccgtatc gccccatccg
      721 ttccttcgtc gcccggcgtc aaaaccaact cggcgccata agcatgcaag atgcgcttgc
      781 gttcctccga cgcgctgtgc ggaagaaaaa gcttcaccgg gtatccgagt gcagcgccaa
      841 tcatcgcgta agcaatgccg gtgtttccgc tagtcgcatc caggatggtc ttgcctggac
      901 gcagtttgcc gctggccagc ccaccttgaa tcatagcaag cgccgcgcgg tcttttactg
      961 acccgcccgg attaaaccat tccgccttag ccagaatttc aaggttaggc aaattccgtg
     1021 ttacccgctc aatccggata agtggcgtgt ttccaatgta ttcgagcagg ttccggccag
     1081 cccgtttttc gttgtccaac gagagtgccg cgccgacttg atgcccctca gccaacccca
     1141 agtcctttag accgtattgg acgccccaat cggtaaaaga tatccgacca gtttactcct
     1201 atttggatgc tttggccgcc acgaaagata aaccggtggg tccggtccac gacgccactc
     1261 tcgtctagct cttgcttagc tgccgaatca gttcagtctc ttccttgcga taaggctggc
     1321 cattcgtgcg gaaaatctcg tggaaccaca ccgcaggctc gcgatccgtg tacggatgct
     1381 cccacgaatc ccatggcaga agcgtctgcg tctttccggc taccagcccc cagttaattg
     1441 cgccaacatg ctcccgcttc gcgatcggta gcgtcccttc aaacgtactc ccattcccgc
     1501 gcgccatgta ctcggtgcaa atcagtggcc gctggtacgc ctgcaaccat ttgattcgtt
     1561 tctcaaactc gtccggcttg tcgtaattgt ggaaagtgat gacgtccgaa ttttccagct
     1621 gaatcttctc cgtggccccc aatttttccg gcgaagacca gtcacccttc cacacaccgc
     1681 tggtgagcgg ctgcgttgca cccgcctcgc gcgcccagcg aaatacttgt ggtaacagag
     1741 tttccactcg ttgtactttg tcgtccggct cctgtgaacc gtacgacccg tgattcgtgt
     1801 tgtccggttc gttccacacg tcccacgtca gcacccggtc atctttccca aacgctttca
     1861 ccactccctt cacataggcc tccaggcgcg aatactgcgc aggatcctcc agcaccgccg
     1921 ctccaggact ctgcacccag cgggaattgt gaatcccagg cctcggcggc aattgcggtc
     1981 ccagtcgcgg cgccggatcc cagcacgaat cgaacagcac aaacatcggt ttgatgtggt
     2041 gcttcttggc gattttcagg aactgatcga tccgctgggt caagcccttc gcgtcctgct
     2101 gccaaaccag gtcatgcaag aaaacgcgca tcgtattcag accgatggtc tcggcccatc
     2161 cgagttccag atcaattcgc ttggggtcaa acgtctccgc ctgccacatc tccagctcat
     2221 tcgaagcggt cgccggcaag tagttgctgc ccaccagcca gccttgcttg gcataccaat
     2281 ctttggcctg ttgctcgctc catctctgtg gagtttccgc gccgctcagc ccggcacctg
     2341 cgaaaacaat cagaacgagc aacaaaagaa agcgcttcat cgtgcatctc ctaggccacg
     2401 ctcgattcga ccttggcagc atataaccga aacactcaaa aatccgctta gcctgcggct
     2461 cactttacag acgccgccgc tttctttaac cttgtgcacc atgtggaatg tcgaacaacg
     2521 ttttgccccg aaggccatcg aaggtttggc tgcccttggt ttttccgtcg gcagtacgcg
     2581 cggcgttgtc cgcgtcgaaa aatacggctg cggcgccgaa tttcgccagg gcactgactc
     2641 ccgctaccag ctcaccgtcc tgcccaccat catggtcaag ggccagttcg cgcgcctgtg
     2701 ggacgccggc taccaaaaat tcttactcac cgacgatggc atcaagctgc ctgccctcgc
     2761 ctggcacttg caaaatctcc gcaagttcaa cgaagaactg cgcaccgcgc tcggtgttcc
     2821 gaccttttac aacgaagcgc tcggttccgt cagccaggtc tcggtctacg atcgcgtgaa
     2881 gggacgcgcg ggcgacgttc ccgacgaaac cgtcggcgct catagtcccg gttccggcca
     2941 ctagcccgga acgccgctct cccgaagggc ctggtcgccc ttcggcggcg atgctggcag
     3001 tgctggcatc agctccgctt ttctcggcat ctattcttta tccg
//
LOCUS       DASTPM010000785         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1325851, whole genome shotgun sequence.
ACCESSION   DASTPM010000785 DASTPM010000000
VERSION     DASTPM010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325851"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFR42_13830"
     CDS             <1..269
                     /locus_tag="VFR42_13830"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEU0370293.1"
                     /translation="PQFSGVSAALSSGSAPSGKVCWIAGAAGTLLRTTDGGNHWEVLH
                     TPISGDLGGVHAADAKRASIWDAPNRLSYETSDGGATWKQTADD"
     gene            364..1881
                     /locus_tag="VFR42_13835"
     CDS             364..1881
                     /locus_tag="VFR42_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370294.1"
                     /translation="MVHRAKSRNRKFLWSSAAAAAILAGAAAVYATPRANHARNEQEK
                     EAAREAQMDHATSLTDQTDLSVTVYNSNIALVRDVRNLSLPSGTFRLKLMDIAATVNP
                     ATVHFRSLNEPDKLGILEQNYEYDLLEPAKLLHKYVGKEVTLVRSYMDNGTTKREEIK
                     ATLLSDNTGPVWKIGNDIVTGGYAESYRFPEVPPNLFDRPTLLMSLENSGSRKQQVET
                     SYLANNLSWNSDYVLTVGRDDKAADLDGWVTLSNNSGTAFHNARLQLVAGDLNRVQPA
                     APVGALRDMAMAKAARAEQFAQENFSEYHLYTLGRKTSVEDKETKQISLLQGSGIPVE
                     KHFVVNGQNFYYHNQQNPGSPIKDNVMVFYKFKNEEKAGLGMPMPAGTVRVYQKDSKG
                     SVLFVGEDRIDHTPKDEALNIHIGNAFDIISERKQTDYKRIDTHTWEMEFEITLRNHK
                     DMPITIEVNEPIGGDWEMVNSTYKYSKTAAWAAQFNVPVASNGISVLKYRIRAHW"
     gene            complement(2103..2501)
                     /locus_tag="VFR42_13840"
     CDS             complement(2103..2501)
                     /locus_tag="VFR42_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0370295.1"
                     /translation="MERQNISSGVKWEPIVGYSRAVRVGNTISVSGTTAAGPDGGIVG
                     VGDARAQTVQTIRNIEAALKQAGATLADVVRTRIYVTDIAEWEKVGRAHGEFFGAIRP
                     ATSMVEVSALISPEMLVEIEADAVVGSAKS"
BASE COUNT          649 a          929 c          624 g          515 t
ORIGIN      
        1 tgccgcagtt cagcggagtt tcggctgctc tctccagtgg ttctgcccct tctgggaaag
       61 tctgctggat cgcgggcgcc gcaggcacgc tcctgcgcac cactgacggc ggcaaccact
      121 gggaagtgct gcacacgccg atttctggcg accttggagg cgtccatgcc gctgacgcca
      181 aacgcgcctc catatgggat gcgcccaacc gtctgagcta cgaaacttcc gacggcggcg
      241 ccacctggaa acaaaccgcc gatgactaag cgttttcgag cgatccgatc agcatagatg
      301 caagaaaatc cgccggtccc gttctaatac cagaaggcaa atcggtactc aggagcgaat
      361 aaaatggtgc acagagcgaa gtcgagaaat cgcaaatttc tttggagttc ggctgctgcc
      421 gccgcaatct tggcgggagc cgcagccgtc tacgcaactc cccgcgcgaa tcacgcccgc
      481 aatgagcagg aaaaggaagc ggcacgcgaa gcacaaatgg atcacgccac cagtcttacc
      541 gaccagaccg acctctccgt gaccgtatac aactccaaca tcgcgctcgt ccgtgacgtt
      601 cgcaatcttt cgcttccgag tggcaccttt cgtctgaagc tgatggacat cgccgccacg
      661 gtaaacccag cgaccgtcca tttccgttcc ctcaatgagc cggacaaact cggcatcctc
      721 gaacaaaatt acgaatacga tctcctcgag cccgccaagc tcctccacaa atacgtcggc
      781 aaagaagtta cgctggtccg ctcctacatg gacaacggca ccaccaagcg cgaagaaatc
      841 aaggccactc ttctgtccga caacactggc ccggtctgga agattggcaa cgacatcgtc
      901 accggcggct atgccgaaag ctatcgcttc cccgaagttc cgccgaatct tttcgatcgc
      961 cccaccctgc tgatgtcgct ggaaaattcc ggctcgcgca aacaacaggt tgaaacttcc
     1021 tatcttgcga acaatttgtc ctggaattcc gactacgtcc tcacggttgg ccgcgacgac
     1081 aaagctgccg acctcgacgg ctgggtcaca cttagcaaca acagcggcac cgcatttcac
     1141 aacgcccgcc tgcagctcgt tgccggtgac ctgaatcgcg ttcagccagc tgcgccggtc
     1201 ggcgctttgc gggatatggc tatggccaag gccgcgcgcg ccgaacagtt cgcccaggaa
     1261 aatttcagcg aatatcacct ctacaccttg ggccgcaaga cttccgtcga agacaaggaa
     1321 accaagcaga ttagcctgct ccagggcagc ggcatccccg ttgaaaaaca cttcgtcgta
     1381 aacggccaga atttctacta tcacaatcag cagaaccccg gctcgcccat caaagacaat
     1441 gtgatggtct tttataaatt caagaacgaa gagaaagccg gcctcggcat gcctatgccg
     1501 gccggcaccg ttcgcgtcta tcaaaaagat tccaaaggca gcgtcctctt cgtcggcgaa
     1561 gaccgcatcg accacacgcc caaagacgaa gccttaaaca ttcacatcgg caacgccttc
     1621 gacatcatca gcgaacgcaa acagaccgac tacaagcgca tcgacaccca cacctgggaa
     1681 atggaatttg aaatcaccct gcgcaatcac aaagacatgc ccatcaccat cgaagtcaac
     1741 gaacccatcg ggggcgactg ggaaatggtc aactccacct acaaatacag caaaaccgcc
     1801 gcctgggccg ctcagttcaa cgtcccggta gcctccaacg gcatttccgt cttgaaatac
     1861 cgcatccgcg cccactggta ggattgctgt ctctaatcaa acaagaaaaa gggcatccgt
     1921 ctcgggtgcc cgcacttttt cgcctcggcc attttgaact tgtcatcctc aagtccggcc
     1981 ctcgcgccgg gccgaacgat ctaaggtaag atcagcggcc cttcacaaag gagcgtctct
     2041 cctcttccac ggaaaacgga acaccgggaa ctgaaaacaa aaaggaactt acaacttaga
     2101 acttatgact tagcacttcc caccaccgca tccgcttcaa tctccaccag catctctggc
     2161 gagatcagcg cgctcacctc gaccatgctc gtcgctggcc gtatcgctcc aaaaaactcc
     2221 ccgtgcgccc gcccgacttt ttcccactcc gcaatgtcgg tcacataaat ccgcgtccgc
     2281 accacatccg ccagcgtcgc acccgcctgc ttcaacgccg cttcgatgtt tcgaatcgtc
     2341 tgcaccgtct gcgcccgcgc atcgcctacg ccgactatcc cgccgtccgg ccccgccgcc
     2401 gtagtccccg acacgctgat cgtattcccc acccgcaccg cccgcgaata acccacaatc
     2461 ggctcccact tcaccccgct ggaaatattt tgccgttcca ttgtcatcct ctcttgcagg
     2521 accgtctgcc gtctaacaag ctcaacttgt actttacgct ttctaccttg tgcggttcac
     2581 cgcaaccacg cgtgctaaac tgactagcgc acatgcgctg caacaacatc ctcgaggcca
     2641 tcggccgcac tcccctcgtc cgtctcaatc gcatcgccca gggcctcagg ccgcagatct
     2701 acgtcaaagc cgattac
//
LOCUS       DASTPM010000786         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_57307, whole genome shotgun sequence.
ACCESSION   DASTPM010000786 DASTPM010000000
VERSION     DASTPM010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57307"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFR42_13845"
     CDS             <1..606
                     /locus_tag="VFR42_13845"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU0370296.1"
                     /translation="DSSDHVTMAFLAERAEFDPNDHSWTLSQAKVVHYDAAGNIISES
                     MEPSVIIRHWSETPYRLRSANMRAELMSLPELRDYLNFNSDFPDTLLAPFRTHYHYRI
                     ALPWTCLVVVLIAAPLGVGFSRRGVLASVAMAILLVFALNFSTHFFLALGEGDRIPAW
                     IAGWAPAIVFGTIGLYLLQLRTSNRDPSDFRWLLPRRILAR"
     gene            603..1115
                     /locus_tag="VFR42_13850"
     CDS             603..1115
                     /locus_tag="VFR42_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370297.1"
                     /translation="MINPLATDWPIKQRSEICNATHRRFTPGEVFYTLLYREGEGFRR
                     EDLSEEAWRDRNENIQPFSFWKTKFEPAPLPAPEALPKENAEQLFRRLIAQPEPPANA
                     CFVLAVMLERKRVLKQVRTEIANGDRLLIYEHRENGDVFIVRDPQLRLSELERVQDQV
                     ATLLRGGAAA"
     gene            1335..>2179
                     /gene="dusB"
                     /locus_tag="VFR42_13855"
     CDS             1335..>2179
                     /gene="dusB"
                     /locus_tag="VFR42_13855"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU0370298.1"
                     /translation="MFTALPWFNAAFPLYLAPMAGVTDKIFRQICKRYGADVLVTEFV
                     SAEGVFRRNERTREYLDFADGERPLGVQLFGANPTHMAEAAKQVVDWVAPDFIDLNFG
                     CPVNKVVAKNGGSALLKDCPTLSSVAKAVVQAVAPLPVTAKIRIGWDANSINAVRVAE
                     ILQGCGIAALAVHGRTRAQGYSGEADWRVIGEVAEGVTIPVIGNGDLFSAQEVVRRRT
                     ETKIAGVMIGRAAMSAPWIFRQIKYYLVTGELLPAPELSERWNVIIEHCRRHADDWGD
                     EEQAIR"
BASE COUNT          441 a          641 c          602 g          495 t
ORIGIN      
        1 gattcctccg atcacgttac catggctttt ttagccgaac gtgcggaatt cgacccgaac
       61 gaccatagct ggaccttgtc gcaggcgaag gtcgtgcact acgatgccgc cggtaatatt
      121 atttccgaat cgatggagcc gagcgtgatt atccggcatt ggagcgaaac accctatcgt
      181 ttgcgcagtg ccaacatgcg cgccgaactg atgagtctcc cagaattgcg cgactactta
      241 aacttcaact ccgattttcc ggacacgttg ctcgcgcctt ttcgaacaca ttatcattat
      301 cgaattgcac tgccgtggac gtgtctcgtc gtcgtactta tcgccgcgcc gctcggggtt
      361 ggtttttcac gccgcggtgt tctcgccagc gtcgccatgg ccattctgct cgtcttcgca
      421 ttgaactttt ccacgcactt tttcctcgct ctgggcgaag gcgaccgtat tcccgcctgg
      481 atcgccggtt gggcacccgc catcgttttt ggaacaatcg gactttattt gctgcaactg
      541 cgcacctcca atcgcgatcc ttccgacttt cgctggcttc tgccgcgccg tatacttgcg
      601 cgatgattaa tccactggcg accgattggc cgatcaagca gcgctccgag atttgcaacg
      661 ccacccatcg acgattcacg cctggggaag ttttctatac tctgctctat cgcgaaggcg
      721 agggtttccg acgcgaagat ttgagcgaag aagcctggcg cgatcgcaat gaaaacatcc
      781 agccgttttc attttggaaa acaaagttcg aaccggcgcc gctgccagct cccgaggcgc
      841 tgccaaaaga aaacgcagag caacttttcc ggcgcctgat agcgcagccg gagcccccgg
      901 caaacgcctg ctttgttcta gccgtcatgc tcgaacgcaa acgcgtcctc aaacaagtgc
      961 gcacagaaat tgccaacggc gatcgcctgc ttatttacga gcaccgggaa aatggcgatg
     1021 tcttcatagt acgagatccg caattgcgcc tgagcgagct cgaacgggtg caggaccagg
     1081 tagcaacgct gctacgcggc ggtgctgccg cgtaagcggc tggaactcag ggcatcctgc
     1141 ctgacccggc cgataagctt ctggcttatc gtgctcgtat tttgcgacgg gcaagacgcc
     1201 cgtcggccga accagccaag gatgcctatc ttcgattcgg gtttaaatca ttccttcgtc
     1261 attcaacgcc agtccggctc ggaccatccg tcattcgtca tttctatcga gcggttttgt
     1321 gagaaaatat tgtgatgttc accgccctcc cttggttcaa tgctgccttc cctctctatc
     1381 tcgcgccgat ggcgggcgtg accgacaaga tttttcgcca gatctgcaaa cgctacggcg
     1441 ccgatgttct tgtcaccgaa ttcgtttcgg ctgaaggcgt ctttcggcgc aacgaacgca
     1501 cccgcgaata tcttgatttc gccgacggcg aacggccgct gggcgtgcag ctttttggcg
     1561 ctaacccgac tcacatggca gaggctgcca agcaggtggt tgattgggtt gccccggatt
     1621 tcatcgacct gaactttggc tgcccggtta acaaagtggt cgcgaaaaat ggcggctccg
     1681 ctctcctgaa agattgtccc acactcagca gcgtggccaa agcggtagtg caagcagtcg
     1741 cgcctttgcc cgtgactgca aaaattcgaa tcggttggga cgccaactcc atcaacgcgg
     1801 tgcgcgtcgc ggaaattctg caggggtgcg gcatcgcggc tctggccgtc catggccgga
     1861 ctcgggcgca aggttattct ggcgaagcag actggcgcgt cattggcgag gtagcggaag
     1921 gggtaacgat accggtaatc ggcaacggcg atttgttttc cgcccaggaa gtcgtgcgcc
     1981 ggcgcactga aaccaaaatt gccggcgtta tgatcgggcg cgcggccatg agcgcgccat
     2041 ggattttccg gcagatcaaa tactatctgg taaccggcga gctgttgccc gcgccagagt
     2101 tatcagagcg ctggaatgtg atcatcgagc attgccggcg tcacgccgac gattggggcg
     2161 acgaggaaca agccatccg
//
LOCUS       DASTPM010000787         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1239437, whole genome shotgun sequence.
ACCESSION   DASTPM010000787 DASTPM010000000
VERSION     DASTPM010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239437"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFR42_13860"
     CDS             complement(<1..404)
                     /locus_tag="VFR42_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370299.1"
                     /translation="MWEEFPGSPPSAKPPDDRLDSWKEIASYLNRDVTTVQRWEKREA
                     MPVHRHLHDRMGSVYASRSELDAWVVSRNPPAAPENGTYPVSPHPRELARSELLRSLT
                     NRTFLLLTAAALVLLLGASLWLQKTEYFWRNPL"
     gene            613..1170
                     /locus_tag="VFR42_13865"
     CDS             613..1170
                     /locus_tag="VFR42_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370300.1"
                     /translation="MRRKLTWFVIIGAGIVVAGLHQAARLLATPANSGFKATTIASGT
                     FEEIDVFNQSSKKDLPAGSDGNVWLSLQKTKGQSDLYVQSNVWQAGGSTGWHTHPGHS
                     LIIVTSGTLTEYEGADCERHVYTFVPGQPAPTLVDGGGGHLHIIRNEGSVAASSIAVQ
                     LVPYDPNKANRRIDAPAPANCAHIL"
     gene            complement(1339..2067)
                     /locus_tag="VFR42_13870"
     CDS             complement(1339..2067)
                     /locus_tag="VFR42_13870"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEU0370301.1"
                     /translation="MPDDSQSELPFERAAEIPVPGINVSVAEQPPAAPDDFSFTIAIG
                     RTANRPPADEAQMFIDVSLPVGQEARPHAPQPLAAEPQPAPIYQHAGQYPVAPIEERR
                     LSGLIDAACLLFACGGFLALFGSLGGHFAFSKLNAAVYAISFAIVYLQYFALFTVFGG
                     TTPGMMLRGLRVVDFSGGEPSPRQLLLRAAGYMLSAGTFFLGFLWVQWDEDELTWHDR
                     ISRTYLSSAETGADSDLGAVAHNH"
     gene            2564..>3437
                     /gene="msrP"
                     /locus_tag="VFR42_13875"
     CDS             2564..>3437
                     /gene="msrP"
                     /locus_tag="VFR42_13875"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEU0370302.1"
                     /translation="MLIKPNSELRIADITPKEVYLNRRKFLQGLGMAGAVVAAGERLY
                     SALTPRVAYAGTKLTTVKSGYTVNEKISSMDDVTHYNNFYEFGTDKGDPAKNAQKFRT
                     SPWTVSVEGEVKTPRKFSMDEIVKLAPLEERIYRHRCVERWSIVVPWIGYSLNTILKM
                     VEPTPKAKYVAFQSFYDHNEMPDSKYTGLEYPYVEGLRLDEAMHPLALLCVGMYGETL
                     PNQDGAPVRMVIPWKYGYKSIKSIVKIKLVGSEPPTTWNKQAPNEYGFYSNVNPNVDH
                     PRWSQATERRLGEVF"
BASE COUNT          781 a          866 c         1174 g          616 t
ORIGIN      
        1 aggggatttc gccagaagta ctcggttttt tgaagccaaa ggcttgctcc aagaaggagg
       61 acgagtgccg ccgccgtcag caggaggaac gtccgattgg tcagagaacg cagcaactct
      121 gagcgcgcga gctcccgggg atgaggcgac acggggtagg tcccattttc cggcgctgct
      181 ggaggattgc gactcactac ccaggcgtcc agttccgagc gagacgcgta gaccgagccc
      241 attcggtcat gcaggtgacg gtgaacaggc atggcctcgc gtttttccca gcgctgaacg
      301 gtggtgacgt cgcggttcag ataagacgct atttccttcc aggaatccag acggtcgtct
      361 ggcggtttcg cggaaggcgg tgacccaggg aattcctccc acatcgcggt ctcctgattg
      421 gggcggaagt tgtcgggcgt tcgagtaggg cgaattgtaa cgttacgtaa cgacaagggc
      481 aaggtacggc atttgacggc agattcgaga aacggcggaa atccgcgcat cgccgtttga
      541 cgctttgccg ccggccgtag gacgttgttc gcgcctccgc aggcaaagac caagcgagaa
      601 ggagagatga acatgcgacg aaaattgacg tggtttgtga tcattggtgc cgggatcgtc
      661 gttgcgggct tgcatcaggc agctaggctg ttggcgacgc ccgcgaattc cggattcaaa
      721 gcgacaacta tagcttcggg caccttcgaa gagatcgatg tgtttaatca gtcgtcaaag
      781 aaggaccttc ccgccggatc cgatggcaac gtctggctgt ccttgcagaa gacgaaagga
      841 cagtccgacc tgtacgtgca gagcaatgtc tggcaggccg gcgggagcac cggatggcac
      901 acacacccgg gccatagcct gattatcgtt acttcgggaa cgctaacgga gtatgagggg
      961 gcggactgcg aacgccatgt ctatacattc gtgccgggac agccagcgcc gacgttggta
     1021 gatggtggag gcggccacct gcacatcatc cgaaacgaag gttcggtcgc ggcatcgagc
     1081 atcgccgttc aactcgtacc gtacgatccg aacaaggcaa atcgccggat tgacgcgcca
     1141 gccccggcaa attgcgccca tatcctgtag cggtatgccc cgatcgatac cacggcgaac
     1201 caaccacaac gcgcctattt gcagacgagt tcgacggctg ttgagctcac ccgcacactc
     1261 ccacacgaga agcagaggga aagcagggtc gtaaaggcca cgaaagggag cctgcccgaa
     1321 ccggagctcg atgtgcgcct agtggttgtg ggcgacggcg ccgagatcgg agtcggcgcc
     1381 agtttccgca gagctgagat acgtgcggga gatgcggtcg tgccaggtga gttcgtcttc
     1441 gtcccactga acccacagga agcctaggaa aaaagtgccg gccgagagca tgtagccggc
     1501 tgcgcggaga aggagttggc gcggggaagg ctctccgcca gaaaaatcga cgacgcggag
     1561 accacgcagc atcatgccgg gcgtggtgcc gccgaaaacg gtgaagaggg cgaagtactg
     1621 cagatagacg atggcgaagg agatggcata gacggcggca ttgagtttgc tgaaggcgaa
     1681 gtgcccgccg agcgagccga acagggcgag gaatccgccg caggcgaaaa ggaggcaggc
     1741 ggcgtcgatg aggccggaga gacggcgttc ttcgatgggc gcgaccgggt attggccggc
     1801 gtgctggtaa atgggcgcgg gttgcggctc agcggctaat ggctgaggtg cgtgaggccg
     1861 cgcttcctgg ccgaccggaa gcgaaacgtc gatgaacatt tgcgcttcgt cagcgggcgg
     1921 gcgattggcg gtgcggccga tggcgatggt gaaggaaaag tcgtcgggcg ctgcgggcgg
     1981 ctgttcggca accgatacgt tgatgccggg cacgggaatt tcggcggcgc gttcgaacgg
     2041 cagctccgac tggctgtcgt cgggcacagc tttgccgcgg cgcgcgcggt aggacgcgat
     2101 gcgctgagag acttcgccgc gccaggccgg atcggcgtgg gcgttcgtag acgaggtccc
     2161 gctggctgag gatgcgggtg actccgcgcg ggagtcacag aaagggcagg ctcgagctct
     2221 ttccagctca gggtctgtaa gtacagaccc tgaaatccga gggagtatcg agccacactg
     2281 caagcatgtt tttgtcaccg gtgcgttttg cgctgcggac gcttccactc cggtgggagt
     2341 gaatcaaccg gagaatagca tgctggggag gagagggagc gagaaaaacg ctgggatttg
     2401 cggaattgtt gccaggacag agaatgggcc gaaaggacag tcaaaaattt tgcaaaattg
     2461 gcggaaacct tgagggcggc agggcatcca acgcgtcggg ggacggcgta ttatttgaag
     2521 agggaaactt gcgggcggcg acaagcggct cgaagcaaaa gagatgctca tcaaaccaaa
     2581 ctccgaactc cgaatcgcgg acattacgcc gaaggaagtg tatttgaacc ggcggaagtt
     2641 tctgcagggg ctgggaatgg cgggcgcggt ggtagcggca ggtgagagat tgtattcggc
     2701 cttaacgccg cgggtggcat acgccggaac gaagctgacg accgtgaaga gcggctacac
     2761 ggtgaatgag aagatctcct cgatggacga tgtgacgcac tataacaatt tttatgagtt
     2821 tgggacggac aagggcgatc ccgccaagaa cgcgcagaaa ttccgcacgt cgccgtggac
     2881 ggtttcggtg gaaggggaag tgaagacgcc acggaagttt tccatggacg aaatcgtgaa
     2941 actggcgccg ctggaagagc ggatttatcg gcaccggtgc gtggagcggt ggtcgatcgt
     3001 cgtaccgtgg ataggatatt cgctgaacac aattttgaag atggtggaac cgacgccgaa
     3061 agcgaagtac gtggcgttcc agagttttta cgatcacaac gaaatgccgg actcgaagta
     3121 caccggcctc gaatatccct atgtagaggg attacggctg gatgaggcga tgcatccttt
     3181 ggcgctgttg tgcgtgggta tgtacggcga gacgttgccg aatcaggatg gcgcaccggt
     3241 gcgcatggtg attccctgga agtatggcta caagagcatc aagtcgatcg tgaaaataaa
     3301 actggtggga tcggagccgc cgactacctg gaacaagcag gctcccaatg aatatggatt
     3361 ttattccaac gtgaatccaa acgtggacca cccgcgctgg agccaggcta cggagcggcg
     3421 gctaggcgaa gtcttca
//
LOCUS       DASTPM010000788         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_490087, whole genome shotgun sequence.
ACCESSION   DASTPM010000788 DASTPM010000000
VERSION     DASTPM010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490087"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..1537)
                     /locus_tag="VFR42_13880"
     CDS             complement(380..1537)
                     /locus_tag="VFR42_13880"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HEU0370303.1"
                     /translation="MIARRLLLLFGLSFLLLTFAAAAVPGKGPAKVPAILPGAFGGWQ
                     MQASAQTSTDPAAADAANPEVLKEYGFTDFASATFSRDDGRKLTIRAARFADASGAYG
                     AFTYYKQPEMLNEKIGDQASSLNNRILFYRGNVLVDALFDKLSVMSAAELRELAGDIP
                     LPQGGARNLPSLPTYLPKPGYVKNTAKYIMGPMTLNKLGSPLPVEMVDFAAGAEVVEG
                     TYHGSGGEGTLMLISYPTPQIATEHLRRIQAAHQPNVQTGLDGSVGLGSFAPKRSGPI
                     VAVAAGTFSQSDAESLASIHYDADVTWNENTFFDKKNNVANLLWNVVLLCGALMAITL
                     VAGLAFGGVRVALRRFLPERSQSVEQEFIALNIDESVSGSRGGMGVSTGSH"
     gene            1738..>2402
                     /locus_tag="VFR42_13885"
     CDS             1738..>2402
                     /locus_tag="VFR42_13885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0370304.1"
                     /translation="MPPKSNNPRLSPGFSRVLVLLFLSVFINYIDRSNLSIAAPLIKD
                     ELGISASQLGILLSAFFWTYSCCQILSGWLVDRFDVKWVFAAGFFLWSSATAVTGLVH
                     SLALLTVVRVVLGIGESVAYPGYSKILAMHCPEGRRGFANSMIAAGLALGPSFGLLFG
                     GTLVARYGWRPFFVVLGTACLLWLVPWVRWMPATSREDFSKSKDGPGLREIVRQRSAW
                     GTCV"
BASE COUNT          493 a          693 c          673 g          543 t
ORIGIN      
        1 acggtgtcca gtcgtctcgg agtattccag gtagacaacc atccgcagcc ggtccgaaac
       61 ggaactgaaa atccggacct gtcgtctgtg gcctgcggga aacaaagagg ccaccccggc
      121 aatagggtgg cctcctcttt caagggagaa tagttctaac ggaaggcaaa gtccgccaaa
      181 actttctggc gaaatcttat gagtcgaaaa agcagggtgt caaggaaatt ttagataata
      241 aatgtctttg ttttcagtgc gttacgaatt gcggcaacat gctgcgcaag agtatcattt
      301 tgagaccttt acgcacttcg cgcgctctaa cgcgtcataa ttgaggtact tacggtattc
      361 cgaaaggtcc gagcgctggt caatgagagc cggtagagac gcccatgccg cctctggagc
      421 cgctaaccga ttcgtcgata ttcagcgcta taaattcctg ctcgacgctt tgtgaacgct
      481 cgggcaggaa gcgtcgtagg gctactcgta ctcctccaaa cgccaaaccc gcaaccaggg
      541 tgatcgccat caacgctcca cacagcaaaa ccacattcca cagcaagttg gcgacattgt
      601 ttttcttatc gaagaaggtg ttttcgttcc acgtcacatc ggcgtcatag tgaatcgagg
      661 ctagcgactc agcgtcgctc tgcgagaatg ttcccgccgc caccgctacg atcggaccac
      721 tccgctttgg cgcgaacgat cccaagccca ctgatccatc aagacccgtc tgcacattcg
      781 gttggtgtgc tgcctgaata cggcggaggt gctcggtggc gatctgcggg gtgggatacg
      841 aaatcagcat cagcgttcct tcgccgcctg acccgtgata cgttccctcg accacctccg
      901 cgcccgctgc aaaatccacc atctccaccg gcaacggcga acccaacttg ttcaacgtca
      961 tcggtcccat gatgtatttg gctgtgttct tgacatagcc gggtttcggc aaatatgtcg
     1021 ggagcgatgg cagattgcgc gcgcccccct gcggaagggg aatgtcgccg gccagctcac
     1081 gaagttccgc cgccgacatc acactcaatt tatcgaacaa agcatccacg agtacattgc
     1141 cgcgatagaa aaggatccga ttattcagcg acgaggcctg atcgccgatc ttctcgttca
     1201 gcatttccgg ttgcttgtag tatgtgaagg caccgtacgc tccacttgcg tccgcaaacc
     1261 gtgccgcgcg gatcgtcagc tttcgtccgt catcgcgtga aaatgtcgcc gaggcgaaat
     1321 ccgtaaaccc gtattccttc agcacctcag ggttggcggc gtccgccgca gcaggatcgg
     1381 tgctggtttg tgccgatgcc tgcatctgcc atccgccgaa cgccccgggg agaattgccg
     1441 gcaccttagc gggtccttta ccaggcacag cagcagccgc aaacgtcaga agaagaaaac
     1501 tgagaccgaa tagaagaaga agtcgacgag caatcataga agtgacaacc ataggcgtga
     1561 cgaagagcaa ccgatcccct gctccctatt agactacaaa gcgaccctga aagtttcccg
     1621 gagggccgat ttctgccggc cggaagtttg aagctagagg cctatccttg ataggcaagt
     1681 caggtcttgc attctcgccg ggcgcgttcc acaatgtgcc gctccccgcg ccagcccgtg
     1741 cctcccaaaa gtaacaatcc gcggctgtct ccgggcttct cgcgggtgct ggttttgctt
     1801 ttcctttccg tcttcatcaa ttacatcgat cgcagcaatc tctcgatcgc cgcgcccctg
     1861 atcaaggacg agctcggcat ctcggcgtcg caactcggca ttctgctctc tgctttcttt
     1921 tggacgtact cctgctgcca gattctgtct ggctggctgg tggatcgctt cgatgtgaaa
     1981 tgggtgtttg cggcgggttt tttcttgtgg tcaagcgcga cggccgtcac cggactggtt
     2041 cactcgctgg ctctcctaac ggttgtccga gtggtacttg ggatcggcga gtcggtcgcg
     2101 taccccgggt actcgaagat cctggccatg cattgcccgg aagggcggcg aggatttgcc
     2161 aattcgatga ttgcagccgg cctggcgctg ggaccgagct tcggcttgtt gtttggcggc
     2221 acgctggtcg cgcgttatgg ctggcggcca ttctttgttg tactggggac agcgtgtctg
     2281 ctgtggctgg tgccgtgggt gcgctggatg ccggcgacta gtcgggagga cttttcgaag
     2341 agcaaagatg gtccgggcct tcgcgaaatc gtgcgccagc gctcggcatg gggcacctgt
     2401 gt
//
LOCUS       DASTPM010000789         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_1095179, whole genome shotgun sequence.
ACCESSION   DASTPM010000789 DASTPM010000000
VERSION     DASTPM010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095179"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2564
                     /locus_tag="VFR42_13890"
     CDS             <1..2564
                     /locus_tag="VFR42_13890"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU0370305.1"
                     /translation="ALDTKLERTVALKFLPDNTAISIGDKNILRREARAASALDHPNI
                     GVIHGLEETSDHHLFIVMQYYEGGTLAQKLSHGCLPLGETLDLAIQIARGLGAAHAQN
                     IVHRDVKPSNIIITKSGAAKIVDFGLARIIASASATQTISATGTLPYMAPEQILGESV
                     DQRSDVWALAVILVQMLTGNHPFVRPSTTAMTFAILNQPPGALDLVPAPLSPILYRAL
                     SKKPEHRYANASEVLTDLEAARAEILDPPSPHGEPTHTHAVTPRELKHFVHNASTPTW
                     TTRTRETKFTRGIAFTSLAIIFIAVAAFLLPPSRERLAGLAYASTEKHIAVLPFTSAS
                     NDPDFQPIAAGLMDSMTNELSNLEAAQGSLWVVPASVVRSRSVNDPATAFRDLGATMV
                     VQGSIARKGSEVSLTVVLIDSRRLRQIGYAQFDNPSGDLATLQNQAVTHLARLMKVSS
                     GSVVTASTGSVAPSAYESYLKALGYMQRYDKPGNPELAIAALKSAVEKDHHFALGYAT
                     LGEAYRLKFLMDHDPASVENALASCRKALEMDERLPTAHVTLGLLQSKLGKDDLASKE
                     FRKVLDINPRDADALIGIAGIYERTNRPPEAETQLKHAISLRPDYWEGYHALAEFYDR
                     QKRPQDSILQYRKIIELTPDNAEAYNALGIEYMQLSDSQSAAAAEAALKKSIQLAPNH
                     LAYMSLGWLYIQQGRYQDAIEPTRKALELNDRDWRTWSNLQVAYTWLKDDKNMLRARA
                     KVVTLLGEYVAVNPNDERSQSMLAMLYAEDKARDKAIFHAKAALALAPNDPWILADIA
                     ETYEDLGDRKTALHYALESFHHGYSVEDLQKYPALHTLLADPGFRAHVMQSSPPQ"
     gene            2586..>2898
                     /locus_tag="VFR42_13895"
     CDS             2586..>2898
                     /locus_tag="VFR42_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370306.1"
                     /translation="MPGTGTTEAKDPRQLEPASLPQFAVITVKNQIPDPRGQVEVTPN
                     IGHIVFQNLDPIQYRIRFFPPGFYDPDAGRNPNTGGTDLLLPANGSLTLIIKAGDEFN
                     YS"
BASE COUNT          635 a         1030 c          657 g          576 t
ORIGIN      
        1 aggccctcga caccaagctc gaacgcaccg tcgccctcaa atttcttccc gacaacactg
       61 ccattagtat cggcgacaag aacattctcc gccgcgaggc tcgcgctgcc tctgccctgg
      121 accatcccaa cattggcgtc atacacggcc ttgaagaaac ttccgatcac cacctcttca
      181 tcgtcatgca gtattacgag ggcggaaccc tcgctcaaaa gctttctcac ggctgcctgc
      241 cactcggcga aactctcgat ctggcgatcc agattgctcg cggcctcggc gccgcccacg
      301 cgcagaacat cgttcaccgt gacgtcaagc cttccaacat cattatcacc aaatccggcg
      361 ccgcaaaaat cgtcgacttt ggcctcgctc gaatcatcgc cagcgccagc gccacgcaaa
      421 ccatctccgc caccggcact cttccctaca tggcccctga acagatcctc ggcgaatctg
      481 tcgaccagcg ctccgatgtc tgggctctcg ccgtcatcct ggtccagatg ctcaccggca
      541 atcacccctt cgtccgcccc agcaccaccg ccatgacctt cgccatcctc aatcagccgc
      601 ccggcgccct tgatctcgtc cccgctcccc tcagccccat cctctatcgc gcgctctcca
      661 aaaagcccga acaccgctac gccaacgctt ccgaagtcct taccgacctt gaagccgccc
      721 gcgctgaaat cctcgatccc ccctcgccgc acggcgagcc tacccacacg cacgccgtca
      781 ctccccgcga actcaagcac ttcgtgcaca acgcttccac gcccacctgg accactcgca
      841 ctcgcgaaac caagttcaca cgcgggatcg cctttacctc tctcgccatc attttcatcg
      901 ccgtggctgc atttctcctt cctccctcac gcgaacgcct cgccggcctc gcctacgcca
      961 gcacggaaaa gcacatcgcc gttctgccct tcacctccgc ttccaacgat cctgatttcc
     1021 agcccatcgc tgccggtctc atggactcca tgaccaatga gctttccaat ctcgaagccg
     1081 cccagggctc tctctgggtt gttccggcca gcgttgtgcg cagccgcagt gtcaacgatc
     1141 cggcgaccgc ttttcgcgat ctcggcgcca ccatggttgt gcagggctct atcgcgcgta
     1201 aaggctccga agtttctctc acggttgtcc tgatcgattc caggcgtctt cgccaaatag
     1261 gctacgcgca attcgataat ccctcgggcg acctcgccac cctgcagaac caggccgtaa
     1321 cgcacttggc ccggttgatg aaggtcagtt ctggtagcgt ggtcaccgcg tccacgggca
     1381 gcgtcgctcc cagcgcctac gagtcctact tgaaggccct cggctacatg cagcgctatg
     1441 acaagcccgg taaccccgag ctcgctattg ctgcgctaaa atccgccgtc gaaaaagatc
     1501 accactttgc cctgggttac gcaactctcg gcgaagctta ccgtctgaaa ttcctgatgg
     1561 atcatgaccc cgcctctgtc gaaaatgccc tcgccagttg ccgcaaagca ctcgaaatgg
     1621 acgaacgtct tccgaccgcc cacgtaacgc tgggcttgct tcagtcgaaa ctcggcaagg
     1681 acgatcttgc ctctaaggaa tttcggaaag ttctggacat caatccccgc gacgccgatg
     1741 ctctgatcgg catcgctggt atttatgaac gcaccaatcg tccgccggaa gccgagaccc
     1801 agctgaagca cgcaatctct ctgcgcccgg attactggga gggctaccac gccctggccg
     1861 agttctacga ccgccagaag cgcccgcagg actccatact tcaataccgc aaaattatcg
     1921 aactgactcc ggacaatgcc gaggcctaca acgcactggg gattgaatac atgcaactgt
     1981 ccgactccca gtccgctgcc gccgctgaag ccgccttaaa gaaatccatt caacttgctc
     2041 ccaatcattt ggcctacatg agtctgggct ggctgtacat tcagcaggga cgctaccagg
     2101 acgccatcga acccacacgc aaggccctcg aattgaacga tcgcgactgg cgcacctggt
     2161 ccaatcttca agtcgcctac acctggctca aggatgacaa aaacatgctc cgcgcgcgcg
     2221 ccaaggtggt gactcttctt ggggagtacg tggcagtgaa tcccaatgac gagcgctcac
     2281 aatctatgct tgccatgctc tacgccgaag acaaggcgcg cgataaagcc atcttccacg
     2341 cgaaagcggc cctcgcgtta gctcccaacg acccatggat tcttgcagac attgccgaaa
     2401 cctatgaaga cctcggcgac cgcaagaccg ctcttcatta cgctctagag agttttcatc
     2461 acggctactc ggtggaggat ctccagaagt atcccgcttt gcacaccctc ctggctgacc
     2521 ccggttttcg ggcccatgta atgcaatcca gtcccccgca ataatccgaa aaggaggcaa
     2581 caaccatgcc tggtacagga accacggaag caaaagaccc acggcaactc gaacccgcat
     2641 cactgccgca atttgcagtc attaccgtta aaaatcaaat tccagatcca cgtggccaag
     2701 tggaggtgac tccgaacata gggcatatcg tcttccagaa cctagaccct atccagtatc
     2761 gcattaggtt ctttccgcca ggcttctatg atcccgatgc cggtaggaac ccgaacactg
     2821 gcggcaccga tcttcttctc ccagcaaacg gatcgttgac gctcatcatc aaggcaggcg
     2881 atgagttcaa ctacagca
//
LOCUS       DASTPM010000790         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_403486, whole genome shotgun sequence.
ACCESSION   DASTPM010000790 DASTPM010000000
VERSION     DASTPM010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403486"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VFR42_13900"
     CDS             complement(<1..833)
                     /locus_tag="VFR42_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370307.1"
                     /translation="MRANFAPKLAARWCVALLLGAGVCVAQEPAVVNQEPPSGPDHPW
                     KSSVYCSGFYTSERVSDDLRLVSGEQSEYKITFVEGNIVYLSKGSSQGVKEGDRFSVI
                     RAEKDPLRVPWFRWQDKLTNAMGTHYSDLGQLTVIKAEPNIAIAKVSMSCEFMQRGDI
                     VLPYSERPAGPFKDPKEFDSLAPSSGKPKAMVVQGKNTAQMSGRWDIVFVNLGSQQGV
                     KVGDYFRAVRYQGTSDQTIPEQKDYQDTMYGFGTSPKHYGWNDLPREVLGEGIVLNVG
                     Q"
     gene            985..>2126
                     /locus_tag="VFR42_13905"
     CDS             985..>2126
                     /locus_tag="VFR42_13905"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU0370308.1"
                     /translation="MPATLATGLPFVNRLCAVIATPSARQALAQLQQALRYTRTLELR
                     LDWLKSDRERSSLLRSLKLRGLQGVTLLATCRRILGGGRLHGGVEAELYWLTQARDAG
                     CQWCDLEIETLRELPGQSARAYPIPRKILLSVHDFKCTPRLPKRLVHAKRGEADSFKI
                     AVKARTLTDSLRVLRLARESQDVVAVPMGEVGLPARLLALREGSALAYAPVAAATAPG
                     QVSLHDFKKLYRADAATRKTKICGVIGNPIAHSLSPLLHNTGYVAAQHDAMFLPFLVE
                     NLNEFVRTIPEFGLRGISVTIPHKQSFLKYLDDCEPMAAKFGAINTVVVAKNGKLLGS
                     NTDYVGVLRALEGKLKLRGARALILGAGGSARAAAFALANAGAEVL"
BASE COUNT          419 a          630 c          619 g          458 t
ORIGIN      
        1 ttttggccca cattcaagac gatgccttcg ccgagcactt cgcgcggcag gtcgttccac
       61 ccatagtgct tcgggctggt gccgaacccg tacatcgtgt cttgataatc cttctgctcg
      121 gggattgttt ggtccgaagt gccctgataa cggaccgcgc ggaagtagtc gcccacttta
      181 acgccctgtt gagagcccag gttcacgaaa acaatgtccc accggccgct catctgcgcg
      241 gtgttcttcc cctgtacaac catcgctttt ggcttgccgc tggagggcgc cagggagtcg
      301 aattccttgg ggtccttaaa cggtcccgcc gggcgctccg agtaggggag aacgatatcg
      361 ccgcgctgca tgaactcgca actcatggag accttggcga tggcgatgtt gggttccgcc
      421 ttgatgacgg tcagctggcc taaatccgaa taatgggtgc ccatggcatt ggtcaatttg
      481 tcttgccacc tgaaccatgg cacgcgcagc ggatctttct ccgcgcgaat cacggagaag
      541 cgatctcctt ccttcacgcc ctggctggag cctttgctaa ggtagacgat attgccttcc
      601 acgaaggtga ttttgtactc ggactgttcg ccgcttacca ggcgcaaatc gtcagacact
      661 ctttcgctgg tgtagaagcc cgagcaatac accgaggact tccacggatg gtcgggaccg
      721 ctcggcggct cctggttcac gactgccggt tcttgcgcca cacaaacgcc tgcgccaagc
      781 agcagggcga cacaccaacg tgcggccagt tttggtgcaa aatttgcgcg catcacaaac
      841 tccttcgcta gacacacgcc cagagggccg tgcggggaca atctcatcac cgaaaatagc
      901 aacgggtttt agtaccgtca acaaaacctc gggacaggct ttacacattt gatacgtcat
      961 gctatatctt ttgttttcaa taatatgcca gcgacccttg cgacaggcct gcccttcgtc
     1021 aaccgtcttt gtgcggtaat cgccaccccc tcggcccggc aagccctggc tcagctccag
     1081 caggcattgc gttacaccag gacgctggaa ctccgcctcg actggctgaa atccgaccgc
     1141 gaacgctcca gcctgcttcg ctctctgaaa ctgcgagggc ttcagggtgt gacgttgctg
     1201 gccacctgcc ggcgaattct gggcggcggt aggctgcacg gcggagtaga agcggaactg
     1261 tactggctca cgcaggcccg cgacgccgga tgccagtggt gcgacctgga aatcgagact
     1321 ttgcgcgaat taccggggca gtccgcccgg gcatatccga ttccccggaa aattttgctc
     1381 tccgtccatg acttcaagtg cacaccaaga ctgcccaaac gtttggtgca cgcaaagcgc
     1441 ggcgaggccg attcgttcaa gatcgcggtc aaggcccgga ccttgacgga tagtttgcgg
     1501 gtattgcgcc tagcgcgtga atcacaagac gttgtcgcgg ttcccatggg cgaggtcggt
     1561 ctgccggcgc ggctgctggc gctgcgtgaa ggcagcgctc tagcttatgc cccggtggcg
     1621 gccgctacgg ctccggggca ggtcagcctc cacgatttca agaagctgta tcgagcggac
     1681 gccgcgacgc ggaagaccaa aatctgtggc gtgatcggaa atcccatcgc gcattctctt
     1741 tcgccgctgc tgcacaacac tggttacgtt gccgcgcagc atgacgccat gttcctcccg
     1801 tttctagtcg agaacctgaa cgaattcgtc aggacaattc cggaattcgg tttgcgcggc
     1861 atcagtgtca cgattccgca caagcaatcc ttcctgaagt atctggatga ttgcgagccc
     1921 atggccgcaa aattcggagc aatcaatacc gttgtggttg cgaagaacgg gaaattgctt
     1981 gggtcaaaca cggattacgt cggcgtattg cgcgcactgg aagggaagct gaagttgcgt
     2041 ggggcgcgag ctctgattct tggcgcgggc ggttcagcgc gtgccgcggc gttcgcgctg
     2101 gcgaatgcgg gcgcggaagt actgat
//
LOCUS       DASTPM010000791         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_403523, whole genome shotgun sequence.
ACCESSION   DASTPM010000791 DASTPM010000000
VERSION     DASTPM010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403523"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VFR42_13910"
     CDS             complement(<1..931)
                     /locus_tag="VFR42_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU0370309.1"
                     /translation="MRAPFYSASKFWLVFCLTFSANLVAGNSGAAQDKPDRPPWAQKP
                     KTAGSDTSQSAPAADESQDSGAQRGRIRVSVNLVNVLVSALDEHNRPAPDLPRESFQL
                     FEEGVEQKIEVFESETQLPLDLVLMIDSSLSAHKEITFEQEAAGHFIGQVLRPGDRLA
                     VYAVGESVTQLAGFSDNVATLQAAVHRMPNDAGTSIYDAVLLAARSLEHRGEDRRKVI
                     ILVTDAGETTSRTDFDAARKEAVRSGSLLYTIVIRPVKNESGRNTAGEHALETITDTM
                     GGAMFYPDSASELTGIFDRINRELRTQYRLGYYP"
     gene            complement(938..1414)
                     /locus_tag="VFR42_13915"
     CDS             complement(938..1414)
                     /locus_tag="VFR42_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU0370310.1"
                     /translation="MPTSHQGDLILLVQKTIDAAALVEHVRTGGDGAIVTFDGCVRNQ
                     SHGRRTLYLEYEAYESMALTKMQEIANEMHSKFAIDRVAMAHRLGRLEIGETSVFIAL
                     SAPHRAAAFDACRYAIDTLKRSVPIWKKEYFEDGAAWADGELPPLPQDTQSRKRAS"
     gene            complement(1423..1737)
                     /locus_tag="VFR42_13920"
     CDS             complement(1423..1737)
                     /locus_tag="VFR42_13920"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0370311.1"
                     /translation="MPRTLAEHSGAGYNSRVSSISTSIRVKVLFFGRLREAAGAAEEL
                     VDMVAGARIEELFATFVARQPELGRYRSSLVASRNREFAAWDTPLHSGDEVAFLPPVS
                     GG"
     gene            1824..>2218
                     /locus_tag="VFR42_13925"
     CDS             1824..>2218
                     /locus_tag="VFR42_13925"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0370312.1"
                     /translation="MKIVLTGASGLVGTALTESLIRDGHAVVRLVRRGSSDKKGGKKN
                     GGDAVNRKPSGLKNVIDVAWNPNTCDLEGEPFGQDQEKIEGADAAVNLAGASIAGESW
                     SDERKALLRSSRVHITRELICALEKLEDGP"
BASE COUNT          451 a          676 c          658 g          433 t
ORIGIN      
        1 tgggatagta gcccaagcgg tactgcgtgc gtagttcgcg attgatgcgg tcgaagatgc
       61 cggtcagctc gctggccgaa tcagggtaga acatcgcccc gcccatcgta tccgtaatgg
      121 tttccagcgc gtgctcgccc gcggtgttgc gcccgctttc gtttttcacc ggtcgaatca
      181 cgatggtata cagcagggaa cccgagcgca ccgcctcttt gcgcgccgca tcaaaatcgg
      241 tgcggctggt agtttcccca gcatcggtca ccaagatgat gaccttgcgc cggtcctccc
      301 cgcgatgctc gagcgagcgc gcggcgagca ataccgcatc gtaaatcgaa gtgccggcat
      361 cgttgggcat gcgatgaacc gcggcctgca gcgtagccac attgtccgaa aaccccgcca
      421 gctgcgtcac gctttcgccc accgcgtaaa cggccagccg atctccggga cgcaacacct
      481 ggccgatgaa atgcccggca gcttcctgtt cgaaagtaat ctccttgtga gcgctgaggc
      541 tggaatcaat catcaacaca agatccaggg gcaactgcgt ttcggactcg aagacctcga
      601 tcttctgctc tacgccttct tcgaaaagct ggaacgattc gcgcggcaag tcaggcgccg
      661 gccgattgtg ttcgtccaat gcgctgacca gcacattgac caaattgacg ctgacgcgaa
      721 tccgcccgcg ctgtgcgccg ctgtcctgcg attcatctgc tgccggggcc gactggctgg
      781 tgtcgctgcc ggctgttttt ggcttttggg cccagggcgg ccggtccggt ttatcttgcg
      841 cagcgccgct atttcccgcc actaaattgg cggaaaacgt taggcaaaac accaaccaaa
      901 atttgctggc ggagtaaaaa ggggcgcgca tcgctagtta ggatgcacgt ttgcggctct
      961 gcgtatcttg cggcagggga gggagttcgc cgtcggccca tgccgcgcca tcctcgaaat
     1021 attctttttt ccagatcggc acggaccgtt tgagcgtatc aatcgcatac cggcacgcat
     1081 cgaacgccgc cgccctatgc ggcgcgctga gcgctatgaa cacgctggtc tcgccgattt
     1141 ccagccgccc caaacggtgg gccatggcta cccgatcaat cgcgaacttg ctgtgcatct
     1201 cgttggcgat ttcctgcatt ttagtcaacg ccatcgattc gtacgcctcg tactccagat
     1261 acagcgtccg acggccatgc gactggttgc gcacgcagcc atcgaaagtg acgatcgcgc
     1321 cgtccccgcc ggtgcgcacg tgctcgacca gcgcagccgc atcgatggtt ttctggacca
     1381 gcaaaatcag atcgccttgg tgggaggtgg gcatcgcgtt gattacccgc cgctgaccgg
     1441 cggaaggaac gccacttcgt ccccggaatg cagcggcgtg tcccaggctg caaactctcg
     1501 attccgtgaa gccacgagtg aactgcgata gcggcccagc tccggttgcc gcgccacaaa
     1561 agtggcaaat aattcttcga tgcgcgcgcc cgcgaccata tcaaccaatt cttcggctgc
     1621 cccagcggct tcccgaagcc gcccgaaaaa gaggaccttc acacgaatgg atgtgctgat
     1681 gctgctcacc cgggaattgt aacctgcgcc gctgtgctcg gccagcgtcc gcggcacaaa
     1741 ggcagagtgt ctttacaggg aaacgcgctt gaccgcgccg ttcggagtgc gtagcgtcct
     1801 ttgtaggagc taacggagag tgcatgaaaa ttgtgctaac aggggcgagc ggacttgtcg
     1861 gcaccgcgct gacggagtct ttaattcgtg acgggcatgc tgtcgttcgt ctggtgcgtc
     1921 gaggaagcag cgacaaaaaa ggcggcaaaa agaatggtgg cgacgccgta aatcgaaagc
     1981 cgagtggcct gaaaaacgta atcgatgtcg cctggaaccc caacacttgc gacctggaag
     2041 gcgagccctt cggccaggac caggagaaaa tcgagggcgc ggacgcggcc gtgaatctgg
     2101 ccggagcctc gatcgccggc gaaagctgga gcgacgagcg caaagcgcta ttgcgctcga
     2161 gccgtgtgca cattactcgc gaactgattt gcgcgctgga aaaactcgag gacggccc
//
LOCUS       DASTPM010000792         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_144018, whole genome shotgun sequence.
ACCESSION   DASTPM010000792 DASTPM010000000
VERSION     DASTPM010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144018"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="VFR42_13930"
     CDS             complement(<1..1890)
                     /locus_tag="VFR42_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370313.1"
                     /translation="MTFVTAPGGYSLLLFPVPPRITMQVTPQLPSMPDRAEIAKRVAQ
                     AEKLLQKGKTGDALEEYLQVLLEDPENDNVRSMASDLCLSLSRTAEAVSLLGDLFERQ
                     IALGDATRASLTYKKLARHGSPTWQQKFRFGQLLENSNKKLAVGTYENALDDLAKQQK
                     KPESLQVMSRIVTLEPTQANYLRMAELCSQLGENKPSAAAFLKTAELAEATGADASQY
                     FERAYSEDPSDPRIALAYGKSLLTQGQIGAAIFIFEPHFIAGNPSPELRDYYCQALLA
                     ANRLAEAEPLLWQLFEQNPGRLQQVVDLIGKLIDSQQDAEAVALARKLELFQRRRGER
                     RAFVAMMQDLTSGHRSSPEMLEFLSELFNSSNRENDYCQTLLKLFDLYVSTGNFQKAG
                     ECLDRAADVDPYERGHQKRLEILKGKIPDDRYKVIASRLSQLNKSVPETARSEEPTLG
                     AAALQDLMLQAEILVQYGMRTKAVERLQRIQELFPREEERNEDLQRLYLAAGVTPKYA
                     GSAPVPAAPSKVSSTPAPLSVPPAVNGEQADVSSFTRVAEITRKLNHQNNANAVLSTA
                     AHEIGAHWKLTRCVAAMRRPGLPPTALQEFHADGLASGEAAALASVVSACQDLVVSQG
                     TVNIPD"
     gene            1942..>2912
                     /locus_tag="VFR42_13935"
     CDS             1942..>2912
                     /locus_tag="VFR42_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU0370314.1"
                     /translation="MPDTPDRSEEKGLRHELGAGQMAMVAVGGSIGTGLLLGSATAIG
                     LAGPAVILSYLLAAFINFTVACALGELACAHPAAGSFGVYGDLYLNDWAGFLSRGGYW
                     AAIAVSIGTELVASAIYMGLWFPAVPRIVWVAAFSIALLLINLVDVGEYGRFEFWFAM
                     VKVVTIASFIALGALLLVNGHTAPQYVSQGGFFPQGFLAPLLAMTFAIYAFGGIEFVA
                     VSSGEARSAHEIANAVRITFAMLTFLYLGAMAILVGVMPWNHAGASESPFVTVFRSVN
                     IPAAGHIMNFVVLTAALSGANAALYVSSRMLFSMARSGWAPARLGQL"
BASE COUNT          516 a          925 c          825 g          646 t
ORIGIN      
        1 atccggtatg ttgacggtgc cctggcttac cacgaggtcc tggcaggccg aaaccacgga
       61 agccagcgcc gccgcttccc cgctggccaa gccatcggca tgaaactcct gcagggcagt
      121 gggtggcagt cccggccgcc gcatggcggc gacgcagcgc gtcagcttcc agtgtgcgcc
      181 aatctcatgc gccgcggtcg acagcaccgc attcgcgttg ttctgatgat tcaacttgcg
      241 cgtaatttcc gcgacccggg taaaactgct gacgtccgct tgctcgccgt ttacggccgg
      301 cggcacgctc aacggagcag gagtcgaaga aactttcgaa ggcgctgccg gcaccggcgc
      361 cgatcccgcg tacttcggcg tcactcccgc cgccaggtaa agccgctgca agtcctcgtt
      421 gcgctcttcc tcgcgcggaa acagttcctg aatacgctgc aaccgctcca ccgcttttgt
      481 gcgcatcccg tactgcacga gaatttcagc ctgcagcatc aggtcctgca atgcggccgc
      541 acccaatgtc ggctcttcgc tgcgcgccgt ctccggaacg gacttgttca actggctcag
      601 acgcgaggca atgaccttgt agcgatcatc cggaatcttt cccttcaaaa tttccaggcg
      661 cttctgatgc ccgcgctcgt acggatcgac atcggccgca cgatccaggc actcgcctgc
      721 cttctgaaaa tttccggtgc tcacatacag atcaaacaac ttgagcaaag tttggcaata
      781 gtcgttctct cgattcgagg aattgaaaag ctcgctcagg aattcgagca tctcgggcga
      841 cgatcggtgc ccgctggtca ggtcctgcat catggccaca aacgctcgcc gctctccacg
      901 gcgccgttga aaaagctcca gcttgcgggc cagcgcgacc gcctcggcat cctgctggct
      961 gtcgatcaac ttcccgatta gatcgactac ctgctgcaag cgtccaggat tctgctcgaa
     1021 caattgccac aacagcggct ctgcctcagc caacctattc gccgccagca atgcctggca
     1081 gtagtaatcc cgcaactccg gcgaaggatt gcccgcgatg aagtgcggtt cgaagatgaa
     1141 aatcgcggca ccaatctgcc cctgcgttag aaggcttttg ccatatgcca gcgcaatgcg
     1201 cggatcgctc ggatcttccg agtacgctct ctcaaaatac tgcgacgcat ccgcgcccgt
     1261 ggcttcggca agctccgcag ttttcaagaa cgccgctgcc gaaggcttgt tctctcccaa
     1321 ctgcgagcag agctccgcca tgcggagata gttcgcctgg gtcggctcga gtgtgacaat
     1381 gcggctcata acctgcagcg actcaggttt tttttgttgc ttggccagat catcaagggc
     1441 gttctcgtag gtgcccaccg ccagtttttt attggaattt tccagcaact ggccaaagcg
     1501 aaacttctgc tgccaggtgg gactgccgtg gcgcgccagt ttcttgtaag tcaggctcgc
     1561 ccgggtcgca tcgcccagcg caatctggcg ctcgaaaagg tcccccagca gagatacggc
     1621 ttcggccgtc cggctcaatg acaggcacag gtccgaagcc atcgagcgaa cgttgtcatt
     1681 ctccggatct tccagcagca cctggagata ttcctccagc gcgtcgccgg ttttgccctt
     1741 ttgcaagagc ttctccgctt gcgcgacacg tttggcgatt tcggctcgat ccggcatgga
     1801 tggtaattgg ggagtaacct gcatggtgat tctaggaggc actggaaaca gcagcaacga
     1861 gtacccgccc ggtgcggtta cgaaggtcat tctcaatttc aactttggga cgtggcgtcc
     1921 ttttctggta tacgtttccg aatgcccgac accccggacc gcagtgaaga aaaaggactt
     1981 cgccacgaac tcggcgccgg gcagatggcc atggtcgctg tcggtggttc cattggcacc
     2041 ggcctcctgc tcggctccgc cactgccatc ggacttgccg gtcccgccgt catcctcagt
     2101 taccttcttg ccgctttcat taacttcact gtcgcctgcg cattggggga actggcctgc
     2161 gcccacccgg ccgcgggatc gttcggcgtg tatggcgatc tctatctgaa cgactgggcc
     2221 ggatttctgt cgcgcggcgg gtactgggcg gccatcgcgg tgtcgattgg caccgagctc
     2281 gtcgcctccg cgatctacat ggggctatgg tttccagctg tcccgaggat cgtctgggtc
     2341 gcggcttttt cgattgccct gctgctgatc aacctggtcg atgtgggcga atatggccgc
     2401 ttcgaattct ggttcgccat ggtgaaggtc gtcaccatcg cgtccttcat cgccctgggc
     2461 gccctgctgc tggtgaatgg tcacaccgct ccccagtacg tctcccaagg aggattcttc
     2521 ccacaaggat ttctcgcccc gcttctggcg atgacgtttg cgatctatgc gttcggaggc
     2581 atcgagttcg tcgcggtgtc gtccggggag gcgcgctccg cgcatgagat cgccaacgcc
     2641 gtgcgcatta cattcgcaat gctgacattc ctttatttgg gggcgatggc gatcctggtc
     2701 ggggtcatgc catggaacca cgcgggcgcc agcgaaagcc ccttcgtcac cgtgtttcgt
     2761 tcggttaaca tccctgccgc gggtcacatc atgaatttcg tggtgctgac cgctgcgctt
     2821 tcgggcgcga atgcggccct gtacgtctcc tcacgcatgc tgttctcgat ggcgcgcagc
     2881 ggatgggcgc ccgcccgcct gggacaactg aa
//
LOCUS       DASTPM010000793         5192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_835956, whole genome shotgun sequence.
ACCESSION   DASTPM010000793 DASTPM010000000
VERSION     DASTPM010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835956"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFR42_13940"
     CDS             <1..539
                     /locus_tag="VFR42_13940"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEU0370315.1"
                     /translation="DVLDLAKEDVRMAKKETKKLDELFHDTLKDIYFAEKKILTTLPK
                     MEKAAQAPQLKRAFAKHKTETEGHVARLEKVFAAIDKKPQGKTCDAIVGITDEGAEIM
                     KEYKGSPALDAGLLAAAQAVEHYEISRYGTLKTWANELGLSNAVGLLDQTLAEEKKTD
                     ATLTEIAESVVNAEAEAA"
     gene            complement(732..1469)
                     /locus_tag="VFR42_13945"
     CDS             complement(732..1469)
                     /locus_tag="VFR42_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370316.1"
                     /translation="MGNISLLQHEHEIERSRAKLTQDLAALCSPQTFAAFTDDLKQQA
                     FAARDAFWEKIKARAASNPAAVAAIGAGLAWRLFQKPPIASALIGLGLFSLWKTQPKT
                     AYDATGRRLDYMEQSKEVLKEQAGQAFSAAADIAGRAQESAAAKGSEVWEGAKEKMRE
                     WQGAVGGSVADTTSTLKSSGEAFLNDIRAKQHDVRDEIQEVATAAMDKIRDDGTRNTL
                     LLGVAGVAIAAALGIACQKRISETLSD"
     gene            complement(1469..2227)
                     /locus_tag="VFR42_13950"
     CDS             complement(1469..2227)
                     /locus_tag="VFR42_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370317.1"
                     /translation="MSTQSRPLLGEGGEWIFNVIKRNPEGLLLLAAGAVLMMRTNSPQ
                     SSQAASIEATYGPTKDAASKTAAQMADAVTDSARRTMDAASSYASTASDTARHTMDAA
                     KSYASSAVEYADQAKHVIGEQSDRVVRQTRSVAQGVLQNQPLAIVAAGLAAGAALAAA
                     FPPTELEKETLGPVGDQMSRAAERFGDQLKQATARAEEKLKTAAEERGLHTEGLKDVA
                     GEVIDTFKASMTGQAEQTNNKGAAPRPDYVDRSK"
     gene            complement(2224..2613)
                     /locus_tag="VFR42_13955"
     CDS             complement(2224..2613)
                     /locus_tag="VFR42_13955"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU0370318.1"
                     /translation="MAEHYSSLPNAFSKVITDLADLMQKEMRLAQAELSEKLSISIRA
                     GVWMSVAAVLAIVGGFLIVQAIVLGLSAATGIALHWSCLIVAAVLAGAAGATFAKGKA
                     DVPHQLTPDRAINQVKQDIAVAKEQFT"
     gene            complement(2606..3142)
                     /locus_tag="VFR42_13960"
     CDS             complement(2606..3142)
                     /locus_tag="VFR42_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370319.1"
                     /translation="MTNQNFEDAESPASGERTKSNKIRDAAGEAFSKASDTARDAGEK
                     AKRAAADAASSMSEQVIGLLNDQLGVGAQSANRFAGSMRMAANDLEHENPMLAGLVRG
                     FAHNVDHYADRLEDQTVEQLAKSAADLTRRQPALMFGLAALAGFFAFRTFKSASASSV
                     ASPPIQPTHYHSAGDNHG"
     gene            3383..4942
                     /locus_tag="VFR42_13965"
     CDS             3383..4942
                     /locus_tag="VFR42_13965"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0370320.1"
                     /translation="MSQAEEEQVVTAFAQQRNVTRDSTPELVSILSTAELNRRPSRPP
                     DHAAENRALGALAQEMAIAPDGILQKLAETALVLCRAHSAGLSLLEDDDQRSAFHWRA
                     IAGQWAPHLNGGTPRNFGPCGTVLDRNIALMCSHPERDFPYFGEVTPLLEEALLIPFY
                     IKGEPVGTIWVVAHDASRRFDLEDLRVMTNLGEFAAAAYQTVLTVNATQRIAFIVESS
                     DDAIISKDLNGIITTWNQGAERIFGYTAEQAIGKPGSILIPPDRQDDEPAILARIRRG
                     ERIDHYETTRLCKHGGRIDISLTVSPIKNAEGKIIGASKIARDITERKQSQVQIATLA
                     REAEHRAKNVLATVQAVVRLSQSDTPAGLKDAIEGRIQALANVHTLFVQSSWTGADLR
                     SLVTQELSPYCSDGETRARFDGLELMLEPNTAQTIAMILHELATNAAKYGALSVPEGR
                     IKVDCSRAEDGGLVLRWAENGGPPVKPPTRRGFGTQVMETMIRTQLKGEMRFDWSADG
                     FACEIALPAGN"
BASE COUNT         1079 a         1537 c         1493 g         1083 t
ORIGIN      
        1 cggacgttct cgacctagca aaggaggacg ttcgtatggc caagaaagaa accaaaaagc
       61 tcgatgagtt atttcacgac acgctgaagg atatctactt cgcggagaag aagatcctca
      121 ccacgcttcc caagatggag aaggcggcac aagcgcccca gctaaagagg gcttttgcca
      181 agcacaaaac agagaccgaa gggcacgtgg cgcggctcga aaaggtcttc gcggccatcg
      241 acaaaaagcc gcaaggcaag acatgcgatg cgattgtcgg catcaccgac gaaggcgcgg
      301 agatcatgaa ggaatacaag ggttcgcccg cccttgacgc cggtcttctc gcagcggcgc
      361 aggcggtcga acactatgaa atcagccgct acggcacact caagacctgg gcaaacgagc
      421 tcggattgag caatgcggtc gggcttctgg atcagacgct ggctgaagaa aagaagaccg
      481 atgcgaccct aactgagatc gcggagagtg tggtcaatgc ggaagcggaa gccgcctgaa
      541 cgcgtgaggt ggacttctcc taaaaggccg ggcgtaggcc gggcctttgg ttttgaaagc
      601 taccgttgtt gaccgccttt ctggtaattt cggcgcggac cgtaggccga tgtcaatgtt
      661 taactcttga gcaatccggc ggcctccggc cgcggcgaac tcgctttcaa cacctcaaat
      721 tccaatgtgt tttagtctga cagagtttca gaaatcctct tctgacaagc gatgccaagc
      781 gcagctgcta tcgcaactcc ggccactccg agcagcagcg tgtttcgagt accgtcgtcc
      841 cgaattttgt ccatagccgc ggtcgccacc tcctggatct catcccgaac atcgtgttgc
      901 ttcgcgcgaa tatcgttcag aaaagcctct ccagaagatt ttagcgttga agtcgtgtca
      961 gcgaccgagc cacccactgc tccctgccac tcccgcattt tttcctttgc cccttcccaa
     1021 acttccgagc ctttcgccgc ggcggattcc tgagccctgc cagcaatgtc tgcggccgct
     1081 gagaacgcct gaccagcttg ttccttgaga acctccttgc tttgctccat gtaatcaagt
     1141 cgtcttccgg tggcgtcata agcggtcttg ggctgagtct tccacaagct gaaaagtcct
     1201 agtccaatca acgcgctcgc aatcgggggc ttctggaata gccgccaagc cagcccggct
     1261 ccgatcgcgg ctacggccgc cgggtttgaa gcggctcgtg ccttgatctt ttcccaaaag
     1321 gcgtctctcg ccgcgaaggc ttgctgtttg agatcatcgg tgaaggccgc gaaagtctga
     1381 ggagagcaaa gagcagccag atcctgcgtt agtttggcgc gcgatcgctc gatttcgtgc
     1441 tcgtgctgca gcagggaaat attgcccatc acttactcct gtcgacataa tctggtcgcg
     1501 gtgccgcacc cttattgttc gtttgctccg cttggcctgt cattgacgcc ttgaacgtat
     1561 caatgacctc tcctgcgacg tccttcaggc cttcggtatg caagccacgc tcctcggcag
     1621 cggtcttgag tttttcttcc gctctcgctg tcgcctgctt gagttgatca ccaaagcgct
     1681 ctgcggctct tgacatctgg tcacccacgg ggccgagcgt ttctttttcc agctcggtcg
     1741 gcgggaatgc cgccgccagt gcggcaccgg ccgcaaggcc agccgccaca atggcaagag
     1801 gttgattttg cagaacaccc tgcgccacag accgcgtctg ccggaccacc cgatcagact
     1861 gctccccgat cacgtgcttc gcctggtcgg cgtactcgac tgccgaagac gcgtaggact
     1921 tagcagcatc cattgtatgc cgggcggtat cggaagccgt tgaggcatag gacgacgccg
     1981 cgtccatcgt ccggcgtgcg ctgtctgtca cggcgtcggc catctgcgcg gccgttttac
     2041 tggccgcatc cttagtagga ccatatgtgg cctcgataga cgccgcttgc gaagattgag
     2101 gagagttcgt gcgcatcatc aacacggcgc cagctgctaa aagcagaagt ccctccggat
     2161 ttctctttat gacgttgaaa atccattcgc cgccctctcc taataagggt cttgattgcg
     2221 tgctcatgtg aactgctcct tggctacggc tatgtcttgc ttgacctgat taatggcccg
     2281 gtccggagtg agctggtggg ggacatcggc ctttcctttg gcaaacgtag cgccagccgc
     2341 acctgccagc accgccgcca cgatcaaaca ggaccagtgc agcgcaatgc ccgtcgcggc
     2401 cgagaggccc aacacgatcg cttgaacgat caggaaccct cccactatgg ccagaacggc
     2461 tgcgaccgac atccagacgc cggcgcgaat gctgatggaa agcttctcag agagttcggc
     2521 ctgggccagt cgcatttcct tctgcataag gtccgctaga tccgtgatga ccttagaaaa
     2581 cgcgtttggc agcgatgaat agtgctcagc catggttgtc gcccgcagaa tggtaatgtg
     2641 ttggctggat cggaggtgac gctacagagc ttgcgctggc acttttgaac gtacggaacg
     2701 cgaaaaaccc agctagcgct gccaggccaa acatcaaagc cggctgccgt cgcgtgagat
     2761 cggcggcact tttggccagc tgttcgaccg tttgatcctc caggcgatct gcgtaatggt
     2821 ccacgttgtg tgcaaatccc ctgacaagac cggcgagcat ggggttttca tgttcgaggt
     2881 cgttagctgc cattctcatc gagccggcga aacggttcgc tgactgtgcg ccaacgccga
     2941 gctgatcgtt aagaaggcct atgacttgct ctgacatgct cgaggccgca tccgcggcag
     3001 cgcgcttggc cttctctccc gcgtcgcgcg cggtatcaga agccttcgag aaagcttcgc
     3061 cggcggcgtc acgtattttg ttacttttgg tgcgctctcc cgatgccggc gactcggcgt
     3121 cttcgaaatt ttgattggtc atttttttct cctgctcaat cccaacaggg ggctaaccct
     3181 ttagttccca aacagcgtgg tcttgcgcga ctgacccgcg gcaaattgag ccgagtggtc
     3241 aattttgaac agccgggtct cctccccctt gctaccctta atgggtcgta cacgccgtgc
     3301 gggcgatctc gccttgcgcg aattgacccg tgcgcatcgc gggacgagac gataaccgtg
     3361 tgggtacgag tctggtgctg cgatgtcgca agcagaagaa gagcaggttg tgaccgcgtt
     3421 tgcgcaacag cgcaacgtga cccgggacag cacacccgaa ctggtgtcga ttctctccac
     3481 ggccgaatta aaccgacgac cgtcacgtcc tcccgatcat gctgctgaga accgcgccct
     3541 gggtgctttg gcgcaggaaa tggcgatcgc acccgacgga attctgcaaa agctcgcgga
     3601 aacggccttg gtcctttgtc gagctcactc ggccgggctc agcctcttgg aggacgacga
     3661 ccaaaggagc gccttccact ggcgcgctat tgccgggcag tgggcgcctc acctcaacgg
     3721 ggggacaccg cgcaacttcg gcccgtgcgg caccgtcctc gaccgcaaca ttgcgctgat
     3781 gtgctcacat cccgagcgcg actttcccta cttcggagag gtcacgccac ttctggaaga
     3841 agcactattg attccatttt acatcaaagg tgagcctgtc ggcacaatct gggtcgtcgc
     3901 ccatgacgca agtcgccgtt tcgacttgga agacctgcgc gtgatgacca acttgggtga
     3961 attcgccgcg gcggcttacc agacagtgct taccgtgaac gcaacgcaac gaatagcctt
     4021 tatcgtggag tcctccgacg acgccattat cagcaaggac ctcaacggca tcatcaccac
     4081 gtggaaccag ggagcggagc ggatctttgg ctatacggcc gaacaggcca tcggcaagcc
     4141 ggggagcatc ttgatcccgc cggatcgcca ggatgacgag ccggcaatcc tcgctcgcat
     4201 tcggcgtgga gagcgtatcg atcattatga aacaactcgt ttgtgcaagc acgggggtcg
     4261 gatcgacatt tcgctaactg tctcgcccat caagaatgcc gagggcaaaa taatcggcgc
     4321 ttcaaaaatc gctcgtgaca tcaccgagcg caagcagagc caagtgcaga tcgcaactct
     4381 cgcgcgcgag gccgagcatc gggccaagaa cgtgttggca acagtgcagg cggtcgtgcg
     4441 tctttcgcaa tccgacacgc ccgccggtct caaagacgcc atcgagggac gcatccaggc
     4501 gctggcaaac gtccatacgc tgtttgtgca atcgagctgg acaggagcag acctgcgcag
     4561 cctcgtcaca caagaacttt cgccctattg cagcgacggg gaaacacgcg cgcgatttga
     4621 cggcctagag ttgatgctgg agcccaacac agcccaaacg attgcgatga ttttgcacga
     4681 acttgccacc aacgcggcca aatatggggc tctctcggtg cccgaaggcc gcattaaagt
     4741 cgactgttcg cgcgcggaag atggcgggct tgtcttgcgc tgggccgaga atggcgggcc
     4801 gcctgtgaag ccgcccacgc gccgagggtt cggcacgcag gtgatggaga ccatgattcg
     4861 aacccaactt aagggcgaga tgcgctttga ttggagcgcg gacgggttcg cgtgcgaaat
     4921 cgccctgccg gcgggaaatt aggagaattc gtcttaaggt tagggccgca cggcagtccg
     4981 gccttatctc tgctttgaaa ggaacatcgg atcaggagcc gcattagccc aagggggcag
     5041 agatttgcgg taatccaatg tcgcgttgcg tactggtggt cgaggacgaa gtcctcgtca
     5101 acatgtttac aagcggtgaa attcaggctg cgggctacga ggttcgcacc gcgttcagcg
     5161 ccgatgaggc gatgcggatt ctggagcagc gc
//
LOCUS       DASTPM010000794         2768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_629809, whole genome shotgun sequence.
ACCESSION   DASTPM010000794 DASTPM010000000
VERSION     DASTPM010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629809"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1429)
                     /locus_tag="VFR42_13970"
     CDS             complement(35..1429)
                     /locus_tag="VFR42_13970"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU0370321.1"
                     /translation="MEPLLLVEDKNELRAMLRKALERNGHSVDEAADGSAAIQKLRNK
                     RYQMVLTDLKMPGASGLDVLRESKQADSTIPVILLTAFGSVDEAVSAMKEGAFDFIQK
                     PVDLDHLKLLVERATRQQELLRENLILREEFASRYGFPRIVGESAAMQAVSQQLQKVA
                     TTEATVLLLGESGTGKELFARAIHHVSNRREQPFVALNCAAIPESLVENELFGHERGA
                     FTGAGNRKVGKIELAQRGTLFLDEIGELPLASQAKLLRVLEERRFERVGGTQSIEVDV
                     RIVVATNRDLAARIREKLFREDLYFRISAVPLTIPPLRDRGDDIDILADHFLEKFSRE
                     LGKPPLTLSADARRRLREYPWSGNVRELQNALERAVILADGPVISRELLVCKPAVVNT
                     DSAPANLVPDSFSWEGSFDEVTARAQRHVERVLLENALKEFHWNKTRAAEKLGITPKT
                     LLSKMRATGLEEKV"
     gene            complement(1459..2040)
                     /locus_tag="VFR42_13975"
     CDS             complement(1459..2040)
                     /locus_tag="VFR42_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370322.1"
                     /translation="MRRIPMPIALAVAMALSCAPTRAQDQSSTGDPVADAARKAREQK
                     KVAQKPKKVFTDDDVKPAEPAPVPPAASDAPAASDEAKPEKSQAKAQGQPEAENPNSE
                     KAWRKRFAEQRKKISDAELKLDVLQREAQKADVQYYSDPQKAMQEQFTRTEINEKNAK
                     IEATKKQIADLKQQLSDMEDQLRASGGDVGWAR"
     gene            complement(2078..>2768)
                     /locus_tag="VFR42_13980"
     CDS             complement(2078..>2768)
                     /locus_tag="VFR42_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0370323.1"
                     /translation="AHEFKNALATISGYAQMIHSDPVAADTAENAAKILDQTRNVTHV
                     VTEFLRYARPLDISAEPVALASLVERVVTEIAELFPQVQIATDGAFAEVAGDDGLLRQ
                     VLLNLVRNAAEAAFAASSRPSVTVHGEILSSPSDGAQRIYIRDNGVGISSEALPKLFL
                     PFFTTKASGTGLGLAIVQKILLQHGGQVEARNGSRGGSGAEIHEGAEFIVTLPATRQI
                     SEAVESKEVAI"
BASE COUNT          534 a          736 c          892 g          606 t
ORIGIN      
        1 aaaccaagta acaaccggaa agcgcaattg cgagtcaaac tttttcttca agtccggtag
       61 ctcgcatttt ggacaataga gtctttgggg tgatgccgag tttttcggcg gcgcgggttt
      121 tgttccagtg gaattccttc aaagcgtttt cgagcagcac gcgctcgaca tgacgctgag
      181 cgcgtgccgt aacttcatca aagctgcctt cccaggaaaa tgaatcgggc acgagattgg
      241 ccggcgcgct gtccgtattg accacggcag gcttgcaaac caggagttcg cggctgatta
      301 ccgggccgtc ggccaggatt acggcgcgct cgagggcgtt ttgtagttcg cggacatttc
      361 cggaccaggg atattcgcgt aggcggcgac gagcgtcggc actcagtgtg agcggaggct
      421 tacccagttc gcggctgaat ttttccagga aatggtcagc aagaatgtca atgtcatcac
      481 cgcgatcgcg cagcggggga atggtgagcg gaacggcgga aatgcggaag tagagatcct
      541 cgcgaaagag tttttcacga atgcgagccg ccagatcgcg gttggtggcg acgacgatac
      601 ggacgtcaac ttcgatggat tgcgtgccgc cgacgcgttc gaagcggcgc tcttcgagaa
      661 cgcgcagcag cttggcctgg ctggcgaggg gcagttcgcc gatttcgtcg aggaaaagcg
      721 taccgcgttg ggcgagttcg attttgccaa ctttgcgatt accggctccc gtgaaggcac
      781 cgcgctcgtg gccgaaaagt tcgttctcga cgaggctttc gggaatagca gcgcaattga
      841 gtgcgacgaa aggctgctcg cggcgattcg aaacgtgatg tatagcgcga gcaaagagtt
      901 ccttgccggt gccgctttcg ccgagcagta ggaccgtggc ttccgtggtg gcgaccttct
      961 gcagttgctg gcttaccgcc tgcatggctg ccgattcgcc gacgatgcgg gggaatccgt
     1021 agcgcgaagc aaactcttcg cggaggatga gattctcgcg gagcagttct tgctggcgcg
     1081 tggcacgctc aaccagcagc ttcagatgat ccaaatccac gggcttctgg atgaaatcga
     1141 aagcaccttc cttcatggcg gagacggctt cgtctaccga accaaacgcg gtaagcagaa
     1201 taacggggat cgtagagtcc gcttgcttgg attcgcgaag aacatcgaga ccggaagcgc
     1261 caggcatctt cagatcggtg agcaccatct ggtagcgctt gttgcgaagc ttctggatgg
     1321 ctgcggagcc gtcggcggcc tcgtcgacag aatggccatt acgctcaagc gccttgcgaa
     1381 gcatagcgcg tagctcattc ttgtcttcga cgaggagcag aggttccatg atgtgcgcgt
     1441 cgggggattc gccaaaatct agcgcgccca gcccacgtcc ccgccgctgg cgcgaagctg
     1501 gtcttccatg tcgctgagct gctgctttag atcggcaatc tgcttttttg tggcctcaat
     1561 cttagcgttc ttttcgttga tttcggtgcg tgtgaactgt tcctgcatgg ccttttgcgg
     1621 atcggagtag tactgaacat cggccttttg cgcttcgcgc tgcaggacgt cgagcttgag
     1681 ttcggcgtcg gagattttct tgcgttgttc ggcgaaacgc ttgcgccatg ctttttcgga
     1741 gttggggttc tcggcttcag gctgaccctg cgccttcgct tgagatttct ccggtttcgc
     1801 ttcgtccgat gcggcagggg cgtcggatgc agccggggga acgggagcag gctcggccgg
     1861 cttgacgtca tcgtcggtga acaccttctt tggcttctgg gctactttct tttgttcgcg
     1921 ggctttacga gccgcgtcag cgacagggtc acccgtggaa gactggtctt gggcgcgagt
     1981 gggcgcgcaa gaaagggcca tcgcgaccgc gagtgcgatc ggcattggaa ttcggcgcat
     2041 aaaaacacct ccggaacgcc acgcgttccc cacaggctta gatggcgacc tcttttgatt
     2101 ctactgcttc agaaatctgg cgtgtagcgg gtagcgtaac gataaactca gcgccctcgt
     2161 ggatctcagc tccggaaccg ccccgtgagc cattgcgtgc ctcgacctgc ccgccgtgct
     2221 gcaaaagaat tttctggacg atggcgagac caagaccggt gccgctcgcc ttggttgtga
     2281 aaaagggaag gaagagcttg ggcaaagcct cggacgaaat gccgacgcca ttgtcgcgaa
     2341 tgtatattcg ctgcgctccg tctgatggag agctgaggat ctcgccgtgc accgtaacgg
     2401 aaggacgcga ggacgcggca aaagccgcct cggcggcgtt gcgtacgaga ttcagcagga
     2461 cctgacggag caggccatcg tcgccagcga cttcggcaaa cgcaccgtcg gtggcaatct
     2521 gcacctgggg aaaaagctcc gctatctcgg taacgacgcg ctcgacgagg gaagcaaggg
     2581 ccactggctc ggcggaaata tcgagcgggc gcgcatagcg taggaattct gtgaccacgt
     2641 gtgtgacgtt gcgggtctga tcgaggattt tggcggcgtt ctcggccgtg tcggcggcga
     2701 ctgggtcgga gtggatcatc tgggcgtaac cggagatggt ggccagggcg tttttgaatt
     2761 cgtgggca
//
LOCUS       DASTPM010000795         3058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_201794, whole genome shotgun sequence.
ACCESSION   DASTPM010000795 DASTPM010000000
VERSION     DASTPM010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201794"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VFR42_13985"
     CDS             <1..1416
                     /locus_tag="VFR42_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0370324.1"
                     /translation="ASRIVFGDAAKVHTDELGRVYVNYHGPSYTYPHYSMADVIERKV
                     KPEAFDGKIVLIGATATGIGDLRTTPFGSHDYPGVEIHANVIDDILNQSFLKRGAKQS
                     LWDFLLILFFGIPFGIWLALVSPRWMWFGFGILALLIGIDYFAFLRGSWLNFTIPALT
                     LTSNVILISLYRALVEEKEKRRVRTAFGQYLSPEVVRRLLVNPQLVEPRKTEITVMFS
                     DIRGFTTISEKLDAQELAIFLNQYLSDMTKIVFERRGTLDKYIGDAVMAFWGAPIEDK
                     EHAVMACQTALAMMARVHELQKEWKAAGKPALDIGIGLNTGTASVGNMGSSLRYGYTA
                     LGDSVNLSSRLEGLNKDYGTHILANETTYAAAKDAGFVFRELDLMRVKGKNQPVVIYE
                     LICDVASLTPEIEMQLDCFENARRLYEKRRWSEAQSAFQSIIDRWPEDGPARAYWKRC
                     QEYLFDEPPAKWDGVFTMTHK"
     gene            1755..>3058
                     /locus_tag="VFR42_13990"
     CDS             1755..>3058
                     /locus_tag="VFR42_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0370325.1"
                     /translation="MWIVRLALQRPYTFIVMALVIVLLTPLVLLRTPTDIFPEINIPV
                     ISLVWTFTGLQPQEMEQRITGNVERGIATLVNDVEHTESQSLNGIAVIKIYFQPNANI
                     QTALAQTAAISQTFLRFLPPGTQPPLVIIYSASTVPVIQLGLTSDTLSEQSLFDFGNN
                     FIRTQLATIPGAATPFPYGGKQRLVSVDTDPMALQSKGLSAVDIVNAVNQQNLILPTG
                     TAKLGSLEYVVEMNGSPKTVNELNDLPVKTVNGATIYMKDVAHIRDGFSPQTNVVLAN
                     GQRGVLMSVYKTGNASTLKIVDNLKALLKYNESSFPEGLKLAQLFDQSLFVRAAIEGV
                     LREALIAAILTAVMILLFLGNWKSTLIIAISIPLSILVSIILLSALGETINIMTLGGL
                     ALAVGILVDDATVEIENINRNLAMGKETVQAILDGAQQIAVPA"
BASE COUNT          680 a          758 c          977 g          643 t
ORIGIN      
        1 gcgtcgcgca tcgtgttcgg cgacgcggcc aaggtgcata ccgacgagct ggggcgcgtt
       61 tacgtgaact atcacgggcc gagctacacg tatccacact attcgatggc ggatgtgatc
      121 gagcggaaag taaagccgga agcgtttgac ggaaagatcg ttttgatcgg agcgacggca
      181 acgggaatcg gggatctgcg gacgacgccg tttggcagcc acgattatcc gggcgtggag
      241 attcacgcaa atgtgattga tgacattctg aatcaaagct tcctgaagcg tggagcgaag
      301 caatcgctgt gggatttcct gctgatcttg ttttttggga ttccattcgg gatctggctg
      361 gcgctggttt cgccgcgctg gatgtggttc ggcttcggaa ttcttgcgct cctgatcgga
      421 atcgattact tcgcattcct gagaggttcc tggctgaact tcacgattcc agcgttgacg
      481 ctgacgtcga acgtcattct gatttcgctg tatcgcgcgc tggtggagga aaaagaaaag
      541 cggagggtgc gcacggcgtt tgggcagtat ttgagcccgg aggtggtacg gcggctgctg
      601 gtgaatccgc aactggtgga accgcgcaag acggaaatca cggtgatgtt cagtgatatt
      661 cgcgggttca cgacgatttc agaaaagctg gacgcgcagg agctggcgat tttcctaaat
      721 caatatcttt ccgacatgac caagattgtg ttcgagcggc gcgggacgct ggacaaatat
      781 attggcgacg cggtaatggc attctgggga gcgccgattg aggacaaaga gcacgcggtg
      841 atggcgtgcc aaacggcgtt ggcgatgatg gcgcgagttc atgaattgca gaaggagtgg
      901 aaagcggcgg gcaagccggc gctggatatc ggaatcgggt tgaataccgg aacggcgagc
      961 gtggggaaca tgggatcgtc gctgcgctac ggatatacgg cgctggggga ttcggtgaac
     1021 ctgtcgtcgc gtctggaagg gctgaacaaa gattacggca cgcacattct ggcgaatgag
     1081 acgacgtacg cggccgcgaa agatgccggg tttgtgttcc gcgaactgga tttgatgcgc
     1141 gtgaagggaa agaaccagcc ggtggtgatt tacgaattga tttgcgacgt ggcgagcctt
     1201 acgccggaga tcgagatgca gctcgattgc tttgagaatg cgcgaaggct ttatgagaaa
     1261 cggcgctggt cggaggcgca atcggcgttt cagtcgatca tcgaccgctg gccggaagat
     1321 ggaccggcgc gagcctactg gaagcgctgc caggaatatt tgttcgacga gccgccggca
     1381 aagtgggacg gcgtgttcac catgacccac aagtaggaac gcgggcgctc cggcagagtt
     1441 atcgctaatg tttcatcttt tcagtccaaa ttggccgaca cgggatatcg ccgtgacccc
     1501 ggcgcggagc ggcgcatcca accttgcgtc tccaaagcca tacgctgagc agaagaggcc
     1561 gccgcggatg gctgagtttt ttaaaagacg agaagcggcg aaagcagtaa tgtatcgtat
     1621 gggtccgaca gaccccaaag gcgtggcgta accaaaagca aagccgcgcg gtctaagtaa
     1681 aagaaaactt cgcgccacga attggcaggt ctgggacccg cgttcttttc ccgactgaag
     1741 gaaaacgaaa cagtatgtgg attgtccgct tagcgctgca gcgtccctac acgtttatcg
     1801 tgatggcgct ggtgattgtc ctgctgacgc cgctggtact gttgcggacc ccgacggata
     1861 tttttccgga gatcaacatt ccggtgatca gcctggtgtg gacgttcacc ggcctgcagc
     1921 cgcaagagat ggagcaacgc atcaccggca acgtggagcg cggcattgcg acgctggtaa
     1981 acgacgtgga gcacacggag tcgcagtcgc taaatggaat tgcggtgatc aagatttatt
     2041 ttcagccaaa cgccaatatc caaacggcgc tggcacagac ggccgcaatc tcgcaaacat
     2101 tcttgcgctt tctgccgccc ggcacccagc cgccgttggt gatcatttat tcggcctcga
     2161 ccgtgccggt gattcagctg gggttgacca gcgacacgct gagcgaacag agcctcttcg
     2221 actttggcaa caacttcatc cgtacgcaac tcgcgacgat tcccggcgcg gcgacgccgt
     2281 ttccgtacgg aggcaagcag cgtctggttt cggtggatac ggatccaatg gcgctgcagt
     2341 ccaaaggcct ttctgcagtg gacatcgtca acgcggtgaa ccagcagaac ctgattttgc
     2401 cgaccggcac ggcgaagctg ggttcgctgg aatacgtggt ggagatgaac ggcagcccga
     2461 aaacggtaaa cgagctgaac gatctgcccg taaagacggt gaacggcgcg acgatttaca
     2521 tgaaggatgt ggcgcacatc cgcgacggat tttcgccgca gactaacgta gtgctggcga
     2581 acggacagcg cggcgtgctg atgagtgtct acaaaacggg taatgcgtct acgctgaaga
     2641 tcgtggacaa cttgaaggcg ctgctcaagt acaacgaatc gtcctttccg gaagggctga
     2701 agctggcgca actgtttgac cagtcgttgt ttgtgcgcgc agcgattgaa ggcgtgctgc
     2761 gcgaagccct gatcgcggcg attttgacgg cggtaatgat cctgctattc ctgggcaact
     2821 ggaagagcac gctgattatt gcgatttcca ttcctctgtc gattctggtc agcatcattt
     2881 tgctgagcgc gttgggcgaa acgatcaaca ttatgacgtt gggcgggctg gccctggcgg
     2941 tcggtatttt ggtcgacgac gcaaccgttg aaattgaaaa catcaaccgc aacctggcga
     3001 tgggtaaaga gacggtgcag gcgattctgg acggcgcgca gcagatcgcg gtcccggc
//
LOCUS       DASTPM010000796         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_629859, whole genome shotgun sequence.
ACCESSION   DASTPM010000796 DASTPM010000000
VERSION     DASTPM010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629859"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /locus_tag="VFR42_13995"
     CDS             complement(<1..2538)
                     /locus_tag="VFR42_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0370326.1"
                     /translation="MKGKITILALAIVGLHIVEATLLGKSASGALLGNVLQIIASFIA
                     TYACFDTARRSARLGQAFWILVGVGISVWGLANIGWTYYQVALGMEPPELSFLRFVFD
                     MQQAFFAMAILLDRDEQTKRVDLGFVLDAVQIALIFLFIYVGLYYIPSLTLEAHGALL
                     REFAITTTEVTAVLLLAVLRAIFAGSIYAKRLYGGLALYLFVYAVGSSVANFAQMSQE
                     KPAGTMLDLAWTAPLLWGALWAAKWKDEKERGAKESPGAKTKTLGEAILTNTLFGAAP
                     VLVFLLSSGLGQEWNRVRYTLLSVSVICYVARMAIADYRQTRAANMVRQQASALDSAV
                     DGMAIVDKDGKYSYVNDGYARLMGNLSREAIIGTAWRATIARDAKTSDEEVREALQKE
                     GKWYGIVEVPRGQTYIPVEVAVTLLADGGVVVIGRDLTERRQAEQAKAEAEIKYRMIV
                     ERVAAISYIAEPGVNGKWYYVSPQMESILGYTPAEWLETSEKWIEFIHPDDHAAVLDA
                     EEIGIRQKSFQAEYRMRRKDGRMVWISDTAVIIQGSDAHPVMEGILVDISERKLMEMQ
                     SQQARRMEAVGRLAGGIAHDFNNLLTIIKGYTELARRRADTPELKTDIDRIEDASERA
                     AALVRQLLAFSRKQVLQPKNLDLNGVVGGLEQLLRRLLGEHVQLQTNLGAKLGTIKAD
                     PSQVEQVLMNLVVNARDAMPEGGRLIIETRNAELDQKYASEHVSVKPGPYVMLAVSDT
                     GVGMSAETVAHIFEPFFTTKGASRGTGLGLATTYGIVKQSGGYIWVYSEPGQGTTFKV
                     YLPRFEEEAEASAATKVKTAAPKGNETILLVEDDEAVRELTEMVLKTY"
BASE COUNT          467 a          931 c          637 g          599 t
ORIGIN      
        1 atatgtcttc agcaccatct ccgtaagttc gcgcaccgct tcatcgtctt ctaccagcag
       61 aatcgtctcg ttgcccttcg gcgccgccgt tttcactttc gtggccgcgc tcgcttccgc
      121 ttcttcctca aaccgcggca aatatacctt gaacgtcgtc ccttgccccg gctcgctgta
      181 cacccagatg tatccgccgc tctgcttcac gattccgtac gtcgtcgcca gtcccagccc
      241 ggttccgcgc gatgcgcctt tcgtcgtaaa gaacggctca aaaatatgcg ccacggtttc
      301 cgcgctcatc cccacgccgg tgtccgacac ggccagcatc acatatggcc cgggtttcac
      361 gctcacatgt tcgcttgcgt acttctgatc cagttccgcg ttgcgcgtct caatgatcag
      421 tcgcccgccc tctggcatcg cgtcgcgcgc gttcaccacc aggttcatca acacctgctc
      481 cacttgcgaa ggatcggctt tgatggttcc cagtttcgcg cccagatttg tctgcagctg
      541 aacatgctcg cccagcagcc gccgcagcag ttgttctaaa ccgcccacta cgccgttcag
      601 atccagattc ttcggctgca gcacctgctt gcggctgaac gccagcaatt gccgcaccag
      661 tgccgcggcg cgttcgctgg cgtcttcgat ccggtcaatg tccgtcttca actccggcgt
      721 gtccgcgcgt cgccgcgcca gctccgtata gcccttgatg atcgtcagca ggttgttgaa
      781 gtcgtgcgcg atgccgcccg ccagccgtcc gaccgcctcc atacgccttg cttgctgcga
      841 ttgcatctcc atcagcttgc gttcgctgat gtccaccaga attccttcca tcacgggatg
      901 cgcatcgctg ccttgaatga tcaccgccgt gtcgctgatc cataccatgc ggccgtcttt
      961 cctccgcatc cggtattccg cttgaaacga tttctgccgt atgccaatct cttccgcgtc
     1021 cagcactgcc gcgtgatcgt ccggatgaat gaactcaatc cacttctccg acgtttccag
     1081 ccattccgcc ggcgtgtaac ctagaatgct ctccatttgc gggctcacgt aataccattt
     1141 gccgtttact cccggctccg caatgtagct gatcgccgcc actcgctcca caatcatgcg
     1201 gtacttgatt tccgcttccg ctttcgcctg ctccgcttgc cgccgctcgg tcagatcgcg
     1261 gccgatcacc accacgccgc catccgccag caaggtcacc gcaacttcca ccggaatgta
     1321 cgtttgaccg cgcggcacct ccacgattcc gtaccacttg ccctcttttt gtagcgcctc
     1381 tcgtacttct tcatcggaag tcttggcgtc tcttgcgatc gtggcccgcc atgctgtgcc
     1441 gatgatcgct tcgcgagaaa ggtttcccat caacctggcg tagccgtcgt ttacatagga
     1501 atattttccg tccttgtcca cgatggccat tccatccact gcggagtcca gcgcgctcgc
     1561 ctgctgccgc accatgttcg cggcccgcgt ttgccgataa tcggcaatcg ccatccgtgc
     1621 cacgtaacaa atcaccgaca cgctcaacag cgtgtacctg acgcggttcc actcctggcc
     1681 caggcccgag gacaacagga acactaggac cggcgccgct ccaaacagcg tgtttgttag
     1741 aatcgcctcg cccagcgtct tcgttttcgc tcctggtgat tcctttgcac cccgttcctt
     1801 ttcgtctttc cacttggctg cccacaatgc tccccacagt aacggcgctg tccacgccag
     1861 gtccagcatc gtccccgccg gcttctcctg gctcatctgc gcgaaattgg ccacgctgga
     1921 gccgaccgca taaacaaaca ggtacagcgc caggccgcca tataagcgct tcgcataaat
     1981 cgagccggca aagatcgcgc gcagcaccgc cagcagtagc accgccgtca cttccgtggt
     2041 cgtgatcgca aactcacgca gcaacgcgcc gtgcgcttcc agcgtcagcg acgggatgta
     2101 gtacagcccc acataaatga acaggaagat caacgcgatt tgcaccgcgt ccagcacgaa
     2161 tcccaggtcg acgcgtttcg tctgttcgtc ccggtccagc agaatggcca tggcgaaaaa
     2221 agcctgctgc atgtcgaaca cgaatcgtag gaaggacagt tccggcggct ccattcccag
     2281 cgccacctgg tagtacgtcc atccgatgtt tgccaggccc cacacgctga tcccgactcc
     2341 caccaggatc cagaacgcct ggccgagcct cgccgatcgc cgcgccgtgt caaagcaagc
     2401 gtacgtcgcg ataaaactgg caataatttg caggacgttt ccgagcagag ccccgctcgc
     2461 ggactttcca agcaaggtcg cctcgacgat gtgcagcccc acaatcgcca atgccaggat
     2521 ggtgatcttt cccttcatcc cacttctttt ctgcgggcgc ccgatcctct ccgcatttac
     2581 gcaggggtgg tacgggcagg cctctcccgg gtttcagggt ttaagggttt gtct
//
LOCUS       DASTPM010000797         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_259473, whole genome shotgun sequence.
ACCESSION   DASTPM010000797 DASTPM010000000
VERSION     DASTPM010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259473"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2756
                     /locus_tag="VFR42_14000"
     CDS             <1..2756
                     /locus_tag="VFR42_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_except=(pos:126..128,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0370327.1"
                     /translation="VNMTPANFSLGGAIHEIEFNHVQQKLMRGLGIVAIENQARIUHS
                     SSVPGLGTRFGRGAATLYPRSMYDSDCVVVMGSNMAENHPVAFRWPMKAKVEHGAKII
                     HIDPRFTRTSAVADIYAPVRAGSDIAFLGGVINYVINSERWNKDPFFKTFVVNYTNAA
                     TLLRDDFKDTEDLDGAFSGLEPAKGGVAEWPFNGFINQYNRASWEYAREKPPAVSPKP
                     VELKPGEGFAPLVASLVWPPPLKDETLQNPRCVFQTVKRHFARYTPEMVEKATGCPKE
                     TFLKVAETILANSGADRTTSFAYAVAWTQHTYGVQIIGACALLQLLLGNIGRPGAGVM
                     ALRGHSSIQGSTDIPTLYHSIQGYMNAPSALRKHDTLQDFIATETIPVGYWANLPKFM
                     VSYLKSIYGDAATKDNQFGYDWHPKILGDHSHLAMFAAMNAGKVKGMICCGQNPAMSL
                     NARLERSALRKLEWLVVKDNWITETANYWKNAPEIKNGEVKMADIKTEVFYLPATQVA
                     ELEGTYTNTQRMLQFHHKAAEAPGDCRSDTQFSYDLGKRLKKLYANSTLPRDEGFKNL
                     VWDYEHEDEAERKKGEPSVRKILKEINGFFTDDPNRHCVGFGDLKDDGSTTCASWIYC
                     GVYPAPDKNLADRRTPDPPGKPGAQLQWGWAWPANRRVMYNRASADPEGKPWSEKKKW
                     VWWDAEAKKWVGYDVPDYIAAKPPDDKAKPGAAGMDALPGTAPFIMRPDGLGWLYVPG
                     GLVDGPLPTHYEPVESPVPNLLYKQQTSPVYKYWKDPGNELAKMGDPKFPYVLTTYRL
                     TEHYLAGGMSRWLPWLAELQPELFVEISPELAQEKGIKNLDWCKISSPRAQIRAKALV
                     TKRMRPFNIDGKVIHQVGMPWHWGYEGLSKGDVVNELTSLIADPNVSIHEGKAFVCNV
                     EKA"
     gene            2845..>3124
                     /locus_tag="VFR42_14005"
     CDS             2845..>3124
                     /locus_tag="VFR42_14005"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0370328.1"
                     /translation="MAEPMGFYTDTTVCIGCKACEVACKEWNQLPSTNGGVNTLSGDS
                     YDNTRRLDGIHWRHVKFIEQFTEDRKDGRWLLMSDVCKHCVHAACLEVC"
BASE COUNT          727 a          917 c          915 g          565 t
ORIGIN      
        1 tcgtcaatat gacgcccgcc aatttctccc tcggcggcgc catccacgaa atcgagttca
       61 accacgtcca acagaaactc atgcgcggtc tcgggattgt agccatagag aaccaggcgc
      121 ggatatgaca cagctctagc gtccccggtc tggggacacg atttggccgc ggcgcggcaa
      181 ccctttaccc ccgaagcatg tacgactccg actgtgtcgt ggtcatgggt tccaacatgg
      241 cggagaacca ccccgtcgcc ttccggtggc cgatgaaggc caaagtcgag cacggcgcca
      301 agattattca tatcgacccg cggttcaccc gcaccagcgc tgtcgcggat atctacgcgc
      361 cggtgcgcgc gggttcggat atcgcctttc tgggcggcgt catcaactat gtgattaaca
      421 gtgagcgctg gaacaaagac ccgttcttca aaacatttgt cgtgaactac acgaacgcag
      481 cgaccctcct cagggatgac ttcaaggaca cggaagattt ggacggagcc ttttccggcc
      541 tggaaccggc gaaggggggc gtagcggaat ggccgttcaa cgggtttatc aaccaataca
      601 accgcgcgag ttgggagtac gcgagggaga agccgcctgc ggtcagccca aagcccgtgg
      661 aactcaagcc gggagaggga ttcgcgccgc tggttgcgtc tctcgtatgg ccgccgccct
      721 tgaaggatga aacgctgcaa aatccacgct gcgtgtttca gaccgtcaag cgccatttcg
      781 cgcgctacac cccggaaatg gtcgagaagg ccaccggctg ccccaaagaa acttttctca
      841 aggtcgccga gaccattctg gccaattcag gcgcggaccg gacgacttca ttcgcctacg
      901 cggtcgcgtg gacgcagcac acctatggcg ttcagatcat cggcgcctgc gcgctgctgc
      961 aacttctgct cggcaacatc ggccggccgg gcgccggtgt tatggcgctc cgcggccact
     1021 cctcgattca gggatcgacg gatattccga cgctctacca ttccatccag ggctatatga
     1081 acgcgccgtc ggcgctcagg aagcacgaca cgctgcagga tttcatcgcc accgagacga
     1141 tacccgtggg ttactgggcg aacctgccga aattcatggt ctcctatctc aagtccatat
     1201 acggcgacgc cgcgaccaag gataaccagt tcggctacga ctggcatccg aagattctcg
     1261 gcgaccactc gcacttggcg atgttcgccg caatgaacgc cggcaaggta aaggggatga
     1321 tctgctgcgg ccaaaacccc gccatgtcgc tcaacgccag gctcgagcga tcggccttga
     1381 gaaagctcga atggctggtc gtgaaggaca attggatcac cgagaccgca aattactgga
     1441 agaacgcgcc ggaaatcaag aacggcgagg tgaagatggc ggacatcaaa acggaagtgt
     1501 tctacttgcc ggccacccag gtcgccgagc tcgaaggcac ctacacgaac acgcagcgga
     1561 tgctgcagtt ccaccacaaa gccgccgaag ccccgggcga ttgccgatct gacacccaat
     1621 tcagttacga tctcggcaag cggctgaaga agctttacgc caattccact ctaccgcgcg
     1681 acgagggttt caagaatctg gtttgggact acgagcacga ggacgaagcc gaacgtaaga
     1741 agggcgagcc ttcggtgcga aaaatcctga aagagatcaa cggcttcttt acggacgatc
     1801 cgaaccgcca ctgcgtcggt ttcggcgacc tcaaagatga cggctcgacg acctgcgcgt
     1861 cgtggatcta ttgtggagtt tatcccgcgc ctgacaagaa cctcgccgat aggcgcacgc
     1921 ccgacccgcc tggcaaaccc ggcgctcaat tgcaatgggg ttgggcatgg ccggcgaatc
     1981 ggcgcgtgat gtacaaccgc gcgtccgccg atcccgaagg gaagccgtgg agcgaaaaaa
     2041 agaagtgggt atggtgggac gcggaggcaa agaagtgggt tggctacgat gtaccggact
     2101 acatcgcagc gaaaccgcct gacgacaaag cgaaacccgg cgccgccggc atggacgcgt
     2161 tgcccggcac ggcgccgttt attatgcgtc cagacgggct cggttggctt tacgtacctg
     2221 gagggctcgt ggacggcccg ctaccgacac actacgaacc ggtcgagtcg ccggtgccaa
     2281 atttacttta caaacagcag acgagcccgg tctacaaata ctggaaggac cccggcaacg
     2341 agttggcgaa gatgggcgat ccgaagttcc cctatgtcct gaccacgtac cggctgactg
     2401 aacactacct ggcgggtgga atgagccgct ggctgccatg gctcgctgag ctgcagcccg
     2461 agctgttcgt tgagatcagt cccgagctag cccaagagaa aggcatcaag aatctcgact
     2521 ggtgtaagat atccagcccg cgcgcgcaga ttcgcgccaa ggcgcttgtc acgaagcgga
     2581 tgcgcccctt caatattgac ggtaaggtca tccaccaagt cggcatgccc tggcactggg
     2641 gttacgaagg actttccaaa ggcgatgtgg tcaacgagct gacatcgctc atagccgatc
     2701 cgaacgtttc gattcacgaa ggaaaagcgt ttgtttgcaa tgtggaaaaa gcgtgaaacg
     2761 tgatcgtgct cgtgactcgt gctcgtcaca aaatcacgaa cacgaaacac gaacacgatg
     2821 gctgggatag gaaaggggta ctttatggct gagccaatgg gattttacac cgacacaacg
     2881 gtctgtatcg gctgcaaagc ctgcgaggtc gcgtgcaaag aatggaatca gcttcccagc
     2941 actaacggtg gcgtcaacac cttgagcggc gacagctatg acaacacgcg caggctggac
     3001 ggcattcatt ggcgccatgt caaattcatc gagcagttca ctgaagaccg caaggacggc
     3061 cgctggctcc taatgagcga cgtgtgcaag cattgcgttc atgcggcgtg cctcgaggtc
     3121 tgcc
//
LOCUS       DASTPM010000798         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_632837, whole genome shotgun sequence.
ACCESSION   DASTPM010000798 DASTPM010000000
VERSION     DASTPM010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632837"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..982)
                     /gene="modA"
                     /locus_tag="VFR42_14010"
     CDS             complement(194..982)
                     /gene="modA"
                     /locus_tag="VFR42_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0370329.1"
                     /translation="MSVAKNRTTLILLAAMLFCIAPPCTAQKSQIRVAAAADLQTALP
                     EVAAAFEASTGTHVELVFGSSGNFVAQIQNGAPFDLFFSADSDYPQKLADSGLALPNT
                     RIVYGLGRIALWMSPNSFCDPRRETWKCLSHPSVKKIAIANPAHAPYGRAAVSSLRVA
                     GLYGQLKDKLVLGENISQAAQFVQSGNAQAGIIAYSLALSPAMRNGKWWEIPAGLYPV
                     IEQSAVILKAAKNQQAAREFLTFVTQGPGRAVLERYGFLAPPVR"
     gene            complement(1006..1413)
                     /locus_tag="VFR42_14015"
     CDS             complement(1006..1413)
                     /locus_tag="VFR42_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0370330.1"
                     /translation="MQRLLSPREAANILGISYPTLKQWIYHGKIQTAKTPGGHHRVPE
                     SEIDRLIPKRLERGEIGLRRGTFRKISGRNQLIGRVVAIKYSGLLAQVTMAIGEQHIT
                     SIITADAAKEMRLKPGERAAALIKSTEVMILRV"
     gene            complement(1498..>2233)
                     /locus_tag="VFR42_14020"
     CDS             complement(1498..>2233)
                     /locus_tag="VFR42_14020"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEU0370331.1"
                     /translation="DPLPEILQLREEFGFRLHADAAYGGYFVLAENLAENARRSFARI
                     GEADSIVIDPHKHGLQPYGCGCVLFRDPAVGRLYKHDSPYTYFSSADLHLGEISLECS
                     RPGASAAALWATQKLLPLVRGGEFAKGLEKGRSAALKFWQVIDQGKTLAAAFEPELDI
                     LCFVPRGGSLAEISSRSRRIFEAAARNGLHLAVAELPVRFWKERKQENGRDTVTCLRS
                     VLMKPDHLEWVEAIVRRLDQAVAEVR"
BASE COUNT          469 a          621 c          678 g          465 t
ORIGIN      
        1 cgtcggcggc aataccaaag gaagcgcaac gacactttcc acaaggaatt tgccgcgcca
       61 tttggtttga gaaagccagt aggcgagcgg catgccggcc agcagcaaac tcaccgcgac
      121 gcaacccgcc aaacgtatgg acaaccagag cgcttccacg atatttacct gcgatgagct
      181 cgatgccgct ggcctaacgc accggtggag cgagaaaacc gtagcgctcc agcacggcac
      241 ggccgggtcc ctgtgttacg aaagtcagaa actcgcgcgc ggcttgctga tttttggcgg
      301 ccttaagtat cacggcgctt tgctcgatca cgggatagag cccggcggga atctcccacc
      361 acttgccgtt gcgcatggcg ggagaaagag cgagcgaata cgcgataatt ccggcttgcg
      421 cgtttcctga ttgcacgaat tgggcagctt gcgaaatatt ttcgccaagt acaagtttat
      481 ccttgagctg gccgtagagc ccggcgacgc gcagggagga caccgcggcg cgtccgtagg
      541 gcgcgtgagc cggattggcg attgcgattt ttttgacgct gggatgcgag aggcatttcc
      601 aggtttcgcg acgcggatca caaaatgagt tgggagacat ccacagcgcg attctgccca
      661 aaccgtagac gattcgggta tttggtaacg cgaggcccga gtccgccagc ttttgcggat
      721 agtcgctgtc cgcagaaaag aagagatcga acggcgcgcc attctggatc tgcgcgacaa
      781 agttaccgct ggagccgaag acgagctcca catgcgttcc ggtgctggct tcgaaggcgg
      841 cagcgacttc gggcagagcc gtttgcaggt cggcggccgc ggcgacgcga atttggcttt
      901 tttgcgcagt gcagggcggc gcgatgcaga acagcatggc ggcgagcagt atgagagtgg
      961 tgcgattttt ggcaaccgac atggtcttgt tcgcccgcgc aaaactcaaa cgcgcaagat
     1021 catcacttcg gtggatttaa ttagcgccgc ggcacgttcg cccggtttga ggcgcatttc
     1081 cttggccgcg tcagcagtaa tgatggaggt gatgtgctgt tcgccgatgg ccatggtgac
     1141 ttgtgcgagg aggccgctgt atttgatggc cacgacgcgg ccgatcaact gattgcggcc
     1201 gctgatcttg cggaacgtgc cgcggcgcaa gccgatttcg ccgcgctcca gtcttttggg
     1261 aatcaggcgg tcaatctcac tttccggaac gcggtggtgg ccgcccggag ttttggcggt
     1321 ctggatcttg ccgtggtaga tccattgctt caaggtggga tagctaatac ccaaaatgtt
     1381 ggccgcttcc cgtggggaga ggagtctctg caccaaaatc ctccgaacta ttgtgcagta
     1441 tagtatacat ccatctgcct ggactatacg tttttatacg attaaagagg aatgcggcta
     1501 tcgcacttcg gcgactgcct ggtccagtct tctgacgatc gcctcgaccc attccaagtg
     1561 gtccggcttc atcagcacgg aacgcaggca cgtgacagtg tctcgaccgt tttcctgctt
     1621 gcgttctttc caaaatcgaa ccggcagctc cgccaccgcc aagtgcaggc cgttacgagc
     1681 cgcggcttca aaaattcgac gcgagcggga ggaaatttcc gccagcgagc caccacgcgg
     1741 gacaaaacag aggatgtcga gctcgggttc aaaggcggcg gccaaggttt taccttggtc
     1801 gataacttgc cagaacttca gagcggcgct tcttcccttt tccagtcctt tcgcgaattc
     1861 tccgccgcgg accagcggca agagtttttg cgtggcccat agagcggctg cggaagctcc
     1921 aggccgagaa cattccaggc tgatttcacc caggtggagg tccgccgaac tgaaataggt
     1981 gtacggcgaa tcgtgtttgt agaggcgacc gactgccgga tcgcggaata gcacgcaacc
     2041 gcagccgtag ggctgcaatc cgtgtttgtg cggatcgatg acgatcgagt ccgcttcacc
     2101 gatgcgcgcg aaactgcgcc gcgcgttctc tgccaaattt tcggccagta caaaatagcc
     2161 gccgtaagca gcgtcggcat gcaagcggaa gccgaactct tcgcgcaatt gcaggatttc
     2221 cggcagcgga tca
//
LOCUS       DASTPM010000799         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_633362, whole genome shotgun sequence.
ACCESSION   DASTPM010000799 DASTPM010000000
VERSION     DASTPM010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633362"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VFR42_14025"
     CDS             complement(<1..652)
                     /locus_tag="VFR42_14025"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370332.1"
                     /translation="MATPEQSPTNSSPIGRDTLRVDGPRKVTGLAKYASDFQFPGVLY
                     AVPVEATIANGKLLTLDTAAAERMPGVRAVLHRGNIGKLFRSTPAPGFDRVCLERRPP
                     FENDAIHYWGQYIALTVADTFEAAKAAADAVRATYSKETPNVEARLEPASDPDVVFTA
                     YGPMERLQSHRGDAASAFDNAAVKLDETYVTPAETHNPIELHSTVALWDGPMLTLYD"
     gene            complement(669..1670)
                     /locus_tag="VFR42_14030"
     CDS             complement(669..1670)
                     /locus_tag="VFR42_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0370333.1"
                     /translation="MHPFEFVRPADQTAAIATAAQSKTAQQGADVRFLGGGTTLIDLM
                     KLNVETPARVLDINRLPLDKIEATSDGGAKIGAMVRNSDLANHAKIQKDYSVLSQALL
                     QGASAQIRNMATVGGNLLQRTRCVYFRDTALACNKREPGSGCPAITGHNRTLAILGAS
                     EHCIATNPSDMCVALAALEATVHVQGPKGSRAVAFGDFHLLPGTTPHRETVLEAGDLV
                     THVTLPPPIAGSKQLYLKLRDRASYEFALASTALVLTIASGKVTRARIALGGVGTKPW
                     RSSEAEAALSGKPATDAIFRQAAEAALRDAKPQSENKFKIELAKRCMVHALRMATAS"
     gene            complement(1686..2321)
                     /locus_tag="VFR42_14035"
     CDS             complement(1686..2321)
                     /locus_tag="VFR42_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0370334.1"
                     /translation="MENDDRFPGPEQPNRRQFLLTTGSTVVGVMAACAPAGATMVAGA
                     KTAAADAAVTAVEGAVPVTLRINGKEHSLRIDPRTTLLDCLRETVTLTGTKKGCDHGQ
                     CGACTVHVNGRRVLSCLNFAVMHDGEEITTIEGLGTPEAMHPMQAAFFTHDAYQCGYC
                     TSGQIMSAVALLKEPCGPDDVAVKELMSGNLCRCGAYANIVAAIQQVRKRA"
     gene            complement(2337..2780)
                     /locus_tag="VFR42_14040"
     CDS             complement(2337..2780)
                     /locus_tag="VFR42_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0370335.1"
                     /translation="MAENVRSHQTGQAVFKAILPDDIDWKPFAALPSSVRLAVMVGQP
                     SEPGPYMIRVKVPGGLKLMPHRHPEDRLYTVISGVFYIGLGDEFNADKLEAYPPGAVI
                     VLPGNTSHFHWAKSGEYVAQVTAVGPLGLEYVNPKDDPRNSNSGM"
BASE COUNT          588 a          860 c          926 g          620 t
ORIGIN      
        1 agtcatacag cgtcagcatg ggaccatccc acagcgccac ggtggagtgc agctcgatgg
       61 gattgtgcgt ttcggcagga gtgacgtacg tttcgtccag cttgacggcc gcattatcga
      121 aagcagatgc ggcgtcgccg cgatggcttt gcaatcgctc catcggcccg taagcagtaa
      181 agacgacgtc aggatcgctg gcgggttcca gacgggcctc cacgttcggc gtttctttag
      241 agtaggtcgc acggacagca tcggccgcag ctttggcagc ctcgaaggtg tcggcaacgg
      301 tcagagcgat gtactggccc cagtaatgga tggcgtcatt ttcgaacggc ggccgccgct
      361 caaggcagac gcgatcgaaa ccaggcgcgg gagtggagcg gaacagcttg ccgatgttcc
      421 cgcgatgaag gacggctcga acgcctggca tcctctcagc cgcggcagta tcgagtgtga
      481 gcaatttgcc attggcgatg gtcgcttcga cgggaacggc gtaaagcacg ccgggaaatt
      541 gaaaatcgga agcgtacttt gccagtccgg tgactttgcg ggggccgtct acgcgaagcg
      601 tgtctcgtcc gataggagaa gaatttgtcg gcgattgctc aggtgtcgcc atggttgctc
      661 ctcagaaatt agctcgccgt ggccattcgt agtgcgtgca ccatgcagcg tttggccagt
      721 tcgattttga atttgttttc gctctgcggc ttcgcgtctc gcagcgccgc ttcggcagct
      781 tgacggaaga ttgcgtcggt tgcgggtttg ccggatagtg cagcttcggc ttcggacgag
      841 cgccatggtt tggttccgac gccaccaagc gcaatgcggg cgcgagtgac cttgccgctt
      901 gcgatcgtaa gcaccagtgc tgtcgatgcc aaggcgaatt cgtacgacgc gcgatcgcgc
      961 agtttcaggt aaagctgctt gcttccggcg attggtggcg gcagtgtaac gtgtgtgacc
     1021 agatcgccgg cttcaagcac ggtctcgcgg tgcggtgtgg tgccgggcag aagatggaaa
     1081 tcgccgaacg cgaccgcacg tgagcctttc ggcccctgaa cgtgaacggt tgcttccagc
     1141 gccgccagag caacgcacat gtccgaggga ttcgtggcga tgcaatgctc gctggccccg
     1201 aggatggcaa gcgtgcgatt gtggccggtg atcgctggac atccactacc aggctcgcgc
     1261 ttgttgcacg cgagagcggt gtcgcggaag tagacgcagc gcgttcgctg cagcaagttt
     1321 ccgccgactg ttgccatgtt gcggatctgt gccgaggcgc cttgcaggag ggcctgcgag
     1381 agaaccgaat agtccttttg gatcttggcg tgattcgcca gatctgagtt gcggaccatt
     1441 gcaccgatct tggcgccgcc atcggatgta gcttcaatct tgtcgagcgg caggcgattg
     1501 atatcgagca cgcgcgcggg agtctcgaca ttcagcttca tcagatcgat cagcgtggtg
     1561 ccgccgccca ggaaacgcac atcggcgccc tgctgcgccg ttttcgattg cgcggcggtt
     1621 gcgatcgccg ccgtctgatc tgccggacga acgaactcga atggatgcat caagaaactc
     1681 ctgcgctaag cgcgcttgcg gacctgctgg atcgccgcaa cgatgtttgc ataggcgccg
     1741 cagcggcaga ggttgccgct catcaattcc ttaaccgcaa cgtcatcggg accgcatggc
     1801 tctttcagca atgcgaccgc ggacatgatc tgacccgaag tgcaataacc gcactgataa
     1861 gcatcgtggg tgaagaacgc ggcctgcatg ggatgcattg cctcaggtgt accgaggccc
     1921 tcgatggtcg tgatttcttc tccatcatgc atgacagcga agttgaggca tgaaagcacg
     1981 cgccggccgt tgacatgcac cgtgcacgcg ccgcactgtc catgatcgca gccttttttc
     2041 gttcccgtga gtgtcaccgt ttcgcggagg caatccagca gtgtggtccg cgggtcgatc
     2101 cgcagcgaat gctccttacc attaatacga agggtgaccg gaactgcacc ttcaacagca
     2161 gtcacagctg cgtcggccgc agcggtcttg gcccccgcaa ccatggtcgc acctgcgggc
     2221 gcgcatgctg ccatgacacc gaccactgtg ctgccggttg tcaacaggaa ttggcggcgg
     2281 ttcggctgtt ccggcccggg gaaacgatcg tcattttcca ttgggtaaac ctcacgctac
     2341 atcccggaat tgctattccg tggatcgtct ttcgggttca catattcaag gcccaggggc
     2401 ccaacggcag tcacctgcgc gacgtactcg cccgacttgg cccagtggaa atgggatgta
     2461 tttccgggta aaacgatcac agcaccgggc ggatacgcct ccagtttatc ggcattgaat
     2521 tcgtcaccaa gaccgatgta gaagactccg gaaatgaccg tgtatagtcg atcctcaggg
     2581 tgcctatgcg gcatcagttt caagccacca ggcactttga ccctgatcat gtaggggcca
     2641 ggttctgaag gctggcctac catgacggca agacgcaccg aggatggaag cgcggcgaac
     2701 ggcttccagt caatgtcgtc cggcaggatt gccttgaaaa cggcttgccc ggtttgatgc
     2761 gatcgaacgt tttctgccac cggaagctcg cgagccaacc tcagccgtgc attgtacgcg
     2821 ttattgctta ggtcgcccag cgtcgacctc accatccagc acgcgtagca ggacttccgg
     2881 tcaggaggac cccagctcgc gaccaggacc caaaccacgc cgatgatggg cgcgcccaga
     2941 gccagaacaa gtcaatgaac gcgggacaat ttgggaagtg ccgacgaatc tgtt
//
LOCUS       DASTPM010000800         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidoferrum sp. isolate SMAG_U2197
            k141_633679, whole genome shotgun sequence.
ACCESSION   DASTPM010000800 DASTPM010000000
VERSION     DASTPM010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960666
            Sequence Read Archive: SRR25158509
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidoferrum sp. (soil metagenome)
  ORGANISM  Candidatus Acidoferrum sp.
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidoferrales;
            Candidatus Acidoferrum.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:56:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,792
            Genes (coding)                    :: 2,780
            CDSs (with protein)               :: 2,780
            Genes (RNA)                       :: 19
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 16
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 0 of 12
            Pseudo Genes (incomplete)         :: 10 of 12
            Pseudo Genes (internal stop)      :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Acidoferrum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633679"
                     /isolate="SMAG_U2197"
                     /isolation_source="soil"
                     /db_xref="taxon:3069120"
                     /environmental_sample
                     /country="China"
                     /lat_lon="33.18166667 N 113.86 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VFR42_14045"
     CDS             complement(<1..847)
                     /locus_tag="VFR42_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0370336.1"
                     /translation="MPGFLVSWLPDFAFMTEPMIGVEGLTKRFGTFTAVDHVSFAVGK
                     GSIFGFLGPNGSGKTTVIRMLCGILEPTDGTAQIGGHDIVRDLDPIKEMIGYMSQKFS
                     LYDELTVNENLIFAGKLYGLSGHELDQRREEMIATTHLEPYIDRRAGQLSGGWRQRLA
                     MACSLMHRPSVLFLDEPTAGIDPVARRELWDLLFEFAGKGMTLFVTTHYMDEAERCDH
                     VGYIYLSKLIVCGEPDELKNLPEVNPPGTRRLDVTCDHVTAALHAMHEMPGVRSATIF
                     GQSMHL"
     gene            complement(906..1778)
                     /locus_tag="VFR42_14050"
     CDS             complement(906..1778)
                     /locus_tag="VFR42_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0370337.1"
                     /translation="MRAWFVRRHAQSLSAAGALALLFLCAGCGTRKNAVSGTIEVDEA
                     HVGPRSGGRVEKILAWEGDRLHEGQVIVQLDASELRARRDLAAAQIDTAVHDADAQEA
                     QLVFLRDEAKRQQDLLQRRVVSSTDAERADSSAKSQEKNVAAAKMRVAQARAQLADID
                     SQLAEMQVVAPADSILEVLSVKVGDVLPANREAATLLLTGHLWVRVYVPESWLGLIKL
                     GEQVRVRVDSFPHTDFDGVVEQINRQAEFTPRNVQTVADRIKQVFGVKVRLPSDDDRL
                     RAGMAADVYFPRVK"
     gene            complement(1759..>2095)
                     /locus_tag="VFR42_14055"
     CDS             complement(1759..>2095)
                     /locus_tag="VFR42_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0370338.1"
                     /translation="RELDEIKPPFVRDMRQKAPQVFQVIERRRAALFQRYFGNLLLAG
                     QRTGMVRKDVRANLIIEILLAIVQGIMNPAKMEELGMMPKEGFAGILKIVLQGALTPK
                     GRKHARMVR"
BASE COUNT          437 a          645 c          559 g          454 t
ORIGIN      
        1 gcaaatgcat cgattggccg aagattgtgg cgctgcgcac gccgggcatc tcgtgcatgg
       61 cgtggagcgc cgcggtcacg tggtcgcaag tcacgtcgag ccggcgtgtt ccgggtggat
      121 tcacctccgg caggtttttt aattcgtccg gctcaccgca cacgatcaat ttcgacaggt
      181 agatgtagcc gacatgatcg cagcgctcgg cttcatccat gtaatgggtt gtgacgaaca
      241 gcgtcatgcc tttaccggcg aactcgaaaa gcaaatccca tagctcgcga cgcgcgactg
      301 gatcgatgcc ggcggtcggc tcatcaagaa agagcacgct cggcctgtgc atgagcgaac
      361 aggccatcgc cagacgttga cgccagccac cggagagctg gccagcgcgc ctgtcgatgt
      421 acggctccag atgcgtcgtg gcaatcatct cctcgcgcct ctggtccaat tcgtgaccgc
      481 tcaagccata gagtttccct gcaaagatga ggttctcatt cacagtcagc tcgtcgtaga
      541 gggaaaattt ttgcgacatg tagccgatca tttccttgat cgggtctaaa tcccgcacga
      601 tatcgtggcc gccgatttgc gccgtgccgt cggttggctc cagaattccg cacagcatgc
      661 ggatcacagt tgttttgccg gaaccatttg ggccgagaaa accgaagatc gatccttttc
      721 cgacggcaaa cgaaacatgg tcaacggcgg tgaaagtgcc gaagcgcttc gtcagccctt
      781 ccactccgat catcggttcg gtcataaaag cgaaatctgg aagccaggaa accaggaagc
      841 cgggcaatca actttttcaa aatctcctgg attcatggtt tccagattca tggatttttc
      901 tgaattcatt tcaccctcgg aaagtaaacg tctgccgcca ttccggcgcg caagcggtcg
      961 tcgtcgctgg gtaagcgaac cttcacgccg aatacctgtt tgatgcggtc agcgacagtt
     1021 tgcacattgc gcggcgtaaa ttcggcctgc cgattgatct gctccacaac gccatcgaag
     1081 tcggtgtgcg gaaacgagtc cacgcggacg cggacttgct cgcccagctt gatcaaaccc
     1141 aaccatgatt cagggacgta aacgcgcacc catagatggc ccgtcaggag cagggttgcg
     1201 gcttcgcgat tcgccggcag cacatcgccc actttgacac tcagcacttc gagaatggaa
     1261 tcggccggcg caaccacctg catttccgcc agctgcgaat cgatatccgc cagctgcgca
     1321 cgcgcctgtg ctacgcgcat cttggccgcc gcgacatttt tttcctgcga tttcgccgag
     1381 ctgtcggcgc gctccgcatc cgtggaggaa accactctcc gctgcagcaa gtcctgctgg
     1441 cgcttcgcct cgtcgcgcag gaacaccagt tgcgcttctt gcgcatcggc atcgtgaaca
     1501 gccgtgtcga tttgcgcggc ggcaagatcg cgacgcgccc gcaactccga cgcatccaat
     1561 tgcacgatga cctggccttc gtgcaaacga tctccttccc acgccaaaat tttttccacg
     1621 cgtccgcctg agcgcgggcc cacgtgcgct tcgtccactt cgatggttcc ggagacggcg
     1681 ttctttcgag tgccgcagcc agcacacaaa aagagcaacg caagagctcc cgccgcactg
     1741 agcgactgtg cgtggcgcct aacgaaccat gcgcgcatgt tttcttcctt tgggtgtaag
     1801 cgcaccttgg agaacaattt taaggatgcc ggcgaaacct tccttcggca tcatcccgag
     1861 ttcttccatc ttcgctggat tcatgatccc ctgaacaatc gccagtagaa tctcgatgat
     1921 aaggttcgcc cgaacgtctt tacgcaccat tcccgtcctt tgtcccgcga ggagaaggtt
     1981 tccgaaatac cgctgaaaca atgcggcccg gcggcgctcg ataacttgaa acacttgcgg
     2041 cgctttctgg cgcatatcac gcacgaacgg cggctttatc tcatccaatt cgcgc
//
